LOCUS       DAOOBH010000001       106801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_160_length_106801_cov_5.638207, whole genome shotgun sequence.
ACCESSION   DAOOBH010000001 DAOOBH010000000
VERSION     DAOOBH010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 106801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 106801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106801
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160_length_106801_cov_5.638207"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..842)
                     /locus_tag="PK218_00005"
     CDS             complement(165..842)
                     /locus_tag="PK218_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPW96925.1"
                     /translation="MKITFYGHACIGVEVGGKNIIIDPFITGNEKAAHIDINAIKADY
                     ILITHAHFDHILDVEVIAKNTNAVIVSNWEIANYFGKKGFQFHPMNHGGSWNFDFGKV
                     KYVNAVHSSAFPDGTYGGNPGGFVIEGEHKNIYISGDTALTMDMKLIPMRTKLDLAIL
                     PIGNCFTMDIEDAIIAADFVACDKILGCHYDTFGFIEINKDDAKKKFFDADKDLMLLD
                     IGESITL"
     gene            complement(859..1782)
                     /gene="menA"
                     /locus_tag="PK218_00010"
     CDS             complement(859..1782)
                     /gene="menA"
                     /locus_tag="PK218_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HPW96926.1"
                     /translation="MKHWIEAARVRTLPLSVSGIIVGSFYAMSQGMFNWNIVAFALLT
                     TLGLQILSNFANDYGDGVKGTDNEDRVGPMRAIQSGVISPQEMKKAMLITSLITLIFA
                     LLLIYFAFKESYLWYSLFFLALGIVAIASAIRYTVGSTAYGYRGYGDLFVFTFFGLVS
                     TFGVYFMFSKSMDWLLLLPATAIGFLSVGVLNLNNMRDEESDKKAGKNTIVVKMGGAW
                     AKKYHFFLVISAMVMVLVFAFLNDFHIDQYIFVVAYFPMISHLMTVARNKNPKLLDSE
                     LKKLAISTFLLSILLALSLIFFISDIVVNYV"
     gene            complement(1928..3427)
                     /locus_tag="PK218_00015"
     CDS             complement(1928..3427)
                     /locus_tag="PK218_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPW96927.1"
                     /translation="MENHFNHPQRQSKIGIIVLFADSLQKYARAFLPIAFIFVLKGDT
                     INVLYLYLGLLAIVVIVGVIAYLRYINFTFYIDDQKEEFIISEGIFNKTRTSIQLNKI
                     QQVNLSQNIVHRIINVYALDVDTAGSDTKEGNIKAVSHEMALALKSKLLENENKVKTT
                     EFTEITDPKTNFSVPFLKIDLVSLFKIGITSNYVRTIGLLLTFFFTIYDGLSQAGKTD
                     VVSSDNVNSLIENKPIIYVILMFIVVMFAVVFILNIARTVIKFFGYTVAKQKGSLVLS
                     YGLINTKSTILKPEKVQITKVSSNYFQKKLNVLEIKIKQAVGHADNKNKNIIEIPGCN
                     SQEADEIIKLLFKELPQEGEMLKPNFRKLGFSIFLLIIVPLSVFYSVGHWVKPVIFDY
                     VSIAVFYAVAITILLCFGFRNYRLFVNDDYIIKQSGAWDVDKEIIQIEKIQALTTSQL
                     FWHKNINIGSLTLHTAGGNVTFYLGKFDRINQLVNLWLYKLETQNSNWM"
     gene            complement(3428..3943)
                     /locus_tag="PK218_00020"
     CDS             complement(3428..3943)
                     /locus_tag="PK218_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPW96928.1"
                     /translation="METFTNEILDLNQLPKHEAISLTPLHPSYWKVMLIKIAIAFLFI
                     AVLAILAINFIDETAGNQTVIWSGYAFLLVIILFFTRLSFKKKAYAFREHDVIFRHGI
                     IATTTMVIPYNRVQHVALHEGLLERNFGLAKVELFTAGGIASDLGVPGILKPQAEDIK
                     QLLMGKIQIQL"
     gene            complement(4019..4858)
                     /locus_tag="PK218_00025"
     CDS             complement(4019..4858)
                     /locus_tag="PK218_00025"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HPW96929.1"
                     /translation="MNTLEWKTVREFEDITYKKLNGVARIAFNRPNVRNAFRPKTTAE
                     LIQAFYDAQEDTSIGVVLLSAEGPSSKDGIWSFCSGGDQKARGHQGYVGDDGYHRLNI
                     LEVQRMIRFMPKAVICVVPGWAVGGGHSLHVVCDMTLASKEHAIFKQTDADVTSFDGG
                     YGSAYLAKMVGQKRAREIFFLGRNYSAQEAFEMGMVNAVVPHDELEQTAYEWAQEVLA
                     KSPTSIKMLKFAMNLTDDGMVGQQVFAGEATRLAYMTEEAKEGRDAFLEKRKPNFEKK
                     WLP"
     gene            4980..6128
                     /locus_tag="PK218_00030"
     CDS             4980..6128
                     /locus_tag="PK218_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPW96930.1"
                     /translation="MKKILVSFLLLFVGIPSFAQPYQFQTIKDIDALPVVSQDKTGTC
                     WSFSSTSFLEAEILRITGKKMDLSEMYNVKYTYLDKAENYVMRQGKAQFGEGGLNHDV
                     INSAKKYGIVYEDCYTGKVNPDEKYDHSKMEAELEAIVKKAVADTPANYPAWKKDYMA
                     VLDNYMGKYDAEDQTVSATATIDKANEKTSKSLSPKQTLELSKLKLDDYITVTSFTHE
                     PAYSKFILNIPDNFSNGSYYNLPLDEFIGTIDYALDNGFTLALDADVSETTFSAKYGI
                     AVLPFNPKDAVEILTSIKPEKKVSAESRQQQFENYSTTDDHLMHIVGKVKDQNGNIYY
                     KVKNSWGTKSGKDGYIYMSVAYMSMKAISVMLDKDGLSKATKKALGLS"
     gene            complement(6175..6684)
                     /locus_tag="PK218_00035"
     CDS             complement(6175..6684)
                     /locus_tag="PK218_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW96931.1"
                     /translation="MITIIPNLDKDFETIRTIAKIVWPVTYSAILTKEQLEYMFDMMY
                     SVTSLQHQAQEKKHHFVIAYEDERPLGFASYEFDCENSNKTKVHKLYILPNLQGKGIG
                     KKIINFIEKEALNNLEKAVYLNVNRFNVAKNFYEKLGFSIVGEVDIEIGNGYLMEDYV
                     MEKWIINKF"
     gene            complement(6685..7140)
                     /locus_tag="PK218_00040"
     CDS             complement(6685..7140)
                     /locus_tag="PK218_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPW96932.1"
                     /translation="MQSLATTPEHYLLDLPEERKVAMQTLRNTILENLPKGFTEKMAY
                     GMIGYAVPHSIYPDGYHCDSKMALPFMSIASQKNFVALYHMGIYADKSLLDWFVAEYP
                     KHCKTKLDMGKSCIRFKKMDDIPFKLIAKLVQKITVDDWISMYESVIKR"
     gene            7215..8306
                     /locus_tag="PK218_00045"
     CDS             7215..8306
                     /locus_tag="PK218_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HPW96933.1"
                     /translation="MSKNTLREDKTCLNCNYVVEKKFCPNCGQENIDTRKTFYQLFVH
                     FFEDLTHYENSFWKTIRNLLLKPASLTKEYLSGKRMSYLAPIRLYIFISFATFFLLSV
                     IPDNEDTPDVKVNSKKALVATKKKEKDTIFKEQEKIIKLEQLGLLTKTESDSLQKLFI
                     QTKLKQIKDKKNNFTLLGSSYNSVEELDSLQKHGKPSEKLGAFEYWITRKMQLVNDKY
                     DSDELKEKFAESFIHNLPKSLFIYMPLFALVLWLFQNKKKWYYFDHGIFTLHYFSFLL
                     LAILILRIINYLFEFINDYTFFNILEYIFATVAILYMFYYFFPAHHRFYGESRVVSAC
                     KGIAMFIINIIFIVIILSVMAIYTFINIH"
     gene            8312..9331
                     /locus_tag="PK218_00050"
     CDS             8312..9331
                     /locus_tag="PK218_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HPW96934.1"
                     /translation="MFKKISTSIFIVFLLVSCSSTKKISSENLTQKYISSISENALKT
                     NLTIIASDEMEGRETGSEGQKKAGRYIIEQYQKAGISFPKGATSYYQTVPAAYLNAKR
                     NENLSESENIWAFIEGTDKKEEIVVVSAHYDHVGIKNGEVFNGADDDGSGTVALIEIA
                     KAFQKAKNEGNGPRRSILILHVTGEEHGLHGSRYYTENPLFPLKNTVADVNIDMIGRR
                     DDLHKDTNNYIYVIGSDYLSSDLYAICESTNQKYIGLTLDYKYNDKKDKNRYYYRSDH
                     YNFAKNGIPSVFFFNGSHADYHKPTDVVDKIEFDALTKRTKYAFAVAWEIAIREKRLV
                     VDKTN"
     gene            complement(9399..9474)
                     /locus_tag="PK218_00055"
     tRNA            complement(9399..9474)
                     /locus_tag="PK218_00055"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9437..9439),aa:His,seq:gtg)
     gene            complement(9538..10254)
                     /gene="bshB1"
                     /locus_tag="PK218_00060"
     CDS             complement(9538..10254)
                     /gene="bshB1"
                     /locus_tag="PK218_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HPW96935.1"
                     /translation="MKLDILCFGAHPDDVELGCSGTIAKEVSLGKKVGIVDLTRGELG
                     TRGSVEIRNAESKKASEILGVTVRENLDMRDGFFINDETHQIKVIQMIRKYKPEIVIC
                     NAIDDRHIDHGKGSKLVSDACFLSGLRKIETSLNNESQEAWRPKVVYHYIQWKNIEPD
                     FVVDISEFIEVKTKSILAYSSQFYTEDSNEPVTPIATKNFLESIHYRSQDFGRLVGVD
                     YAEGFTVERYLAVNSLSDLK"
     gene            complement(10283..11347)
                     /locus_tag="PK218_00065"
     CDS             complement(10283..11347)
                     /locus_tag="PK218_00065"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HPW96936.1"
                     /translation="MTDLFLKIKTHQDQNLPFVIFCKPNSEKTIGLFQKNDHLYFLES
                     FDKKGFVFAAFDNSTTPFLPLEFCDVFVEKNNSETYFFDAIQNENSPKQGNDFFEELV
                     TKSVAAIDNGLFQKVVTSRREIVELNSFDLEVCFKRMLQNYPSAFKYCFYHPKIGLWM
                     GATPEQLVKVKELEFETVALAGTQKANDIKVVKWSPKEEVEQQLVTDFIVENTKEFTK
                     ELNVSEPFTANAGSIFHIKTTISGKLKNKKAVEKIINALHPTSAVCGMPREAAKDFIL
                     QHEGYNREFYSGFLGELNIDFATFRTQYSDLFVNLRCMKIKENRAEIFVGCGITKDSN
                     PKAEYIETVNKAMTMKRCIL"
     gene            complement(11340..11771)
                     /locus_tag="PK218_00070"
     CDS             complement(11340..11771)
                     /locus_tag="PK218_00070"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPW96937.1"
                     /translation="MSFDKDKIINLCNELCKNTMMESLEIEFIDAGEDFLTAKMPVNS
                     KVHQPMGLLHGGASVALAESVGSAASIMYVNPEKQEVRGIEISANHVRSKREGIVFGT
                     ARIVHKGRSIHLWEIKITDEQDRLISICKLTTMVLPKRHND"
     gene            11946..13466
                     /locus_tag="PK218_00075"
     CDS             11946..13466
                     /locus_tag="PK218_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW96938.1"
                     /translation="MRNFIILLFIGISISLSSCRQDFVFEASTGDLTFSKDTIYLDTV
                     FTNIGSSTYTLKVYNRSNKDIKIPLIKLGKDDSKYRITVDGMTGENNHTFRDVEMLAK
                     DSMYIFVEVTADVADSNPADFIYTDQIQFDTGTNFQKVDLVTLIQDAYFIYPSRTQNP
                     DNSYTYEEINLGVDADGNPIPFRASVLDENDPVNGNEFIWNNSKPYVIYGYAVVPDGK
                     TLNIEPGAQIHFHADSGLIVGTGGTLKSIGTLQNQIVFEGDRLEPIFSDVPGQWGTIW
                     LTAGSTANEIDYTTIKNATVGLLIDSNDGNFMTIKNTQIYDSSNLGIYAKTAKIKGEN
                     IVINTAGVASLACTLGGSYEFKHCTFNNNWSSSKQVAVLLDNYYRDENNAQVPFDLIQ
                     ADFINCIIYGSNTYELRFDKGETGTFNSSFTKCLVKFGTSNDALYSFIYDENNIKRNK
                     NPKFLNIFTNQLNIGVDSEAKEFGVDADVPLDILGMPRTAPYDVGAYNWVTFPEDN"
     gene            13509..14141
                     /locus_tag="PK218_00080"
     CDS             13509..14141
                     /locus_tag="PK218_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HPW96939.1"
                     /translation="MKYKTLTVALALLCFVSCKKEAAKTETETTTEPTEEVTMTMPDS
                     ATIAKAWQDYATPGEQHALMAKDNGSWTEDMTYWEHAGAEPSKMTMTAESKMIMGGKF
                     QEMTHKGELYGMPFEGISTFGYNNAEEKYISTWIDNMSTGITVLKGTYDAPTKTYKMI
                     GEVIDPITKKPKTSRETITIIDDNTQKLEMFDVGFDGKEFKNMEIIMKRK"
     gene            14252..17917
                     /gene="purL"
                     /locus_tag="PK218_00085"
                     /gene_synonym="purI"
     CDS             14252..17917
                     /gene="purL"
                     /locus_tag="PK218_00085"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPW96940.1"
                     /translation="MIHFFGNESATVFAIQSQNELSAETISKLNWLLGNTTKIDKSVL
                     ADFFVGPRAAMITPWSTNAVEITQNMGIEGIIRIEEFEKVTQDFTDFDPMLSQKYSEL
                     HQDIFFINVEPEPILDIDDIAAYNQQEGLALSSEEVDYLNTLSTKLNRKLTDSEIFAF
                     SQANSEHCRHKIFNGTFVIDGEEKPTSLFKLIKKTSETNPNDIVSAYKDNVAFVKGPR
                     VTQFAPKSADKADFYEEKEFDSVLSLKAETHNFPTTVEPFNGAATGSGGEIRDRLAGG
                     QGSLPLAGTAVYMTSYSRLRKDRNWENGMKKRPWLYQTPMDILIKASNGASDFGNKFG
                     QPLITGSILTFEHEENNRKIGYDKVIMQAGGIGYGKLDQAIKKKPHEGDKIVVLGGEN
                     YRIGMGGAAVSSADTGAFGSGIELNAIQRSNPEMQKRAANAIRGLVESDTNPIVSIHD
                     HGAGGHLNCLSELVEDTGGLIDLDKLPVGDPTLSYREIIGNESQERMGLIIGEKDMAI
                     LKRIAERERSPMYEVGEVTNDHRFTFESKSTGAKPMDYALEDFFGSSPKTIMTDKHIA
                     THYADVSYTAENIPTYLEEILKLEAVACKDWLTNKVDRCVGGKVAKQQCAGAIQLPLN
                     NVGVMALDYNGKEGIATSIGHSPISALVNPAAGSRNAIGEALSNLVFAPLKENLKSVS
                     LSANWMWACKNEGEDARLYEAVQACSDFAIELGINIPTGKDSLSMKQKYPNDEVIAPG
                     TVIISAAGHCSNITKVVEPVLQKNGGAIYYINLSQDDFKLGGSSFAQTHNKIGKDVPT
                     IKDSAFFKKAFNTIQELIKDRQIVAGHDIGSGGLITTLIEMCFADTNLGATISFEALP
                     EKDLVKILFSENIGVVLQAKDDAAFEKAFEGLEFFKIGAATNSDSLVVSGFKFQVSSY
                     RKLWFETSFLLDQKQTKNNKAQERFDNLGKQALEYTFPSHFTGAMPSKITSTAHGVTR
                     PKAAIIREKGSNSEREMANAMYLAGFDVKDIHMTDLISGRENLEDIQFIGAVGGFSNS
                     DVLGSAKGWAGAFLYNEKANTALKNFLKRDDTLSVGICNGCQLFMELGVINPEHEVHG
                     KMKHNDSHKHESGFTSVTIEENNSVMLSTLAGATLGVWISHGEGKFHLPLPEAQYNIV
                     SKYAHEGYPANPNGSDYNTAMLCDKTGRHLVMMPHIERSTFPWNWAHYPTDRKDKVSP
                     WLEAFVNARLWIEKHNS"
     gene            17930..18364
                     /locus_tag="PK218_00090"
     CDS             17930..18364
                     /locus_tag="PK218_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW96941.1"
                     /translation="MTLKNAISALLLLITMTTFAQNSLTEIETDKGIRYITNSIDYQI
                     TGKYSFKGGDPIAVLNNDGTGIYQLHDQPQRAMTWGIECTKNGELKFIKGFDNVKYAL
                     FYKFDTSLDGETVENWNKVDFTIHLNSLKMFINGEREKNFNP"
     gene            18787..19914
                     /locus_tag="PK218_00095"
     CDS             18787..19914
                     /locus_tag="PK218_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HPW96942.1"
                     /translation="MSKLPHIGTSIFTVMSKMAIDYKAINLAQGFPNFPVDERLTGIV
                     SGIATENVHQYAPMSGHTALLDKIKQLTFSSYSRNVNPTTEILVTAGATEGIFATIQA
                     LVYRNEEVIILDPSYDCYETPIILCNAKPIRIALKDDFTPDWETIGNAINHTTKLIII
                     NNPHNPSGRVWTEYDYNQLEKLLDSYPNLLVLSDEVYEHITFEQKHLSIHHRPKLWDR
                     SISVSSFGKTFHITGWKIGYLIAPEPLMNEIKKVHQFLVFSVNSIAQTALAEYLNVVD
                     VPALSSFYQQKRDYFRALIKDTKFTLLPCEGTYFQTVSYNNISTENDIDFTKRLVTDY
                     GVATIPISAFNFNGKDTNCIRFCFAKDDATLESAAEKLMKL"
     gene            19982..20863
                     /locus_tag="PK218_00100"
     CDS             19982..20863
                     /locus_tag="PK218_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW96943.1"
                     /translation="MNFSAKMLRDNALSDKVIVVTGGGSGLGKAMTKYFMELGAKVAI
                     TSRDLEKLKKTATELEAETGGTCLPVQCDVRHYDQVDAMLAEVLKAFGKVDVLLNNAA
                     GNFISPTERLSANAFDTIIDIVLKGSKNCTLAFGKHWIDQKQTNCNILNIVTTYAWTG
                     SGYVVPSATAKAGVLAMTRSLAVEWAKYGIRMNAIAPGPFPTKGAWERLLPGDLAEKF
                     DMAKKVPLRRVGDHQELANLAAYLVSDFSAYINGEVVTIDGGEWLQGAGQFNILEKIP
                     QEMWDMLEEMIKSKGSK"
     gene            21022..21789
                     /locus_tag="PK218_00105"
     CDS             21022..21789
                     /locus_tag="PK218_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW96944.1"
                     /translation="MGKIIAIANQKGGVGKTTTSINLAASLGVLEKKVLLIDADPQAN
                     ATSGLGIDVESVEIGTYQILEHSYTPKDAIVASTSPNVWVLPAHIDLVAIEIELVDKD
                     KREYMLKQALEEVKDDYDYILIDCAPSLGLLTLNALTAADSVIIPIQCEYFALEGLGK
                     LLNTIKSVQKIHNPELDIEGLLLTMFDSRLRLSNQVVEEVQKHFSDMVFKSIIQRNVK
                     LSEAPSFGESIINFDATSKGASNYLSLANEVIQKNNN"
     gene            21793..22692
                     /locus_tag="PK218_00110"
     CDS             21793..22692
                     /locus_tag="PK218_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPW96945.1"
                     /translation="MAKAVQKQALGRGLSALLKDPENDIKSVSDKNADKVVGNIIELE
                     IDAIEINPFQPRTNFNEESIKELASSIKELGVIQPITVRKLDFNKYQLISGERRLRAS
                     KVVGLTTIPAYIRIANDNESLVMALVENIQRHDLDPIEIALSYQRLIDEIQLTQEQMS
                     ERVGKKRSTIANYLRLLKLDPIIQTGIRDGFISMGHGRALISIENLDVQSDIYHKIVM
                     ENLSVRETEALVKNHQDSLKPAPAKSKKTNNFSIDESQKKDIAAFFGAKIDVKVAGNG
                     KGKITIPFHSEEDLNRIINLLTK"
     gene            22692..23258
                     /locus_tag="PK218_00115"
     CDS             22692..23258
                     /locus_tag="PK218_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPW96946.1"
                     /translation="MNKAFYIVLFFFLLGSQTFFGQVSSSDLVIAKDTAKVSKTIVYD
                     PLRPSKAAFYSALLPGLGQAYNKSYWKIPIVYGAIGTSLYFYFNNNKKYHSYRDAYKQ
                     RLQGLPDQYDYLDDDRLIRAQRFYQRNRDLSLLVAVGFYVLNIVDANVDAHLTQFNVN
                     DNLSIVPDLQQSDFTVLPNLGLTLNYKF"
     gene            23259..23966
                     /gene="dapB"
                     /locus_tag="PK218_00120"
     CDS             23259..23966
                     /gene="dapB"
                     /locus_tag="PK218_00120"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPW96947.1"
                     /translation="MKIALLGYGKMGHVIERIALERGHEIVLKKTSSNSFDGLEKADV
                     AIDFSIPTVAVENISTAINHGIPVISGTTGWLENYHEMVKLCEEKNAAFLYGSNFSLG
                     VNLFFELNDYLAKMMSKFKEYNVSMEEIHHTQKLDKPSGTAISLANAIINHTDKNNWA
                     IENPTPEAVFIDVKREENVPGTHSVLYTSEVDYIKIEHVAHNREGFALGSIIAAEWII
                     GKKGVFNMKDVLSLNSK"
     gene            24049..25647
                     /gene="lepB"
                     /locus_tag="PK218_00125"
     CDS             24049..25647
                     /gene="lepB"
                     /locus_tag="PK218_00125"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPW96948.1"
                     /translation="MSIYQWFVFLLILQLIHGLGTWKLYEKAGRKSWEAFIPVYNSIV
                     LMKIINRPTWWTLLLFIPIINLIMIPVIWVETMRSFGKNSTVDTWLTLFTFGFYIYYV
                     NYTQDVTHIKDRNLVATTKVGDTVSSLLFAVVVATLVHTYVMQPYTIPSSSLEKSLLI
                     GDFLFVSKFHYGARIPMTAVAAPMVHDTLPFINTKSYLYDDNDATSWKNKLQFPYFRF
                     PGFQDIKNNDIVVFNWPRDTLFNMYKNADRRYDKPIDKKTNYVKRCVGIPGDSLQIKD
                     GIVYINGKELILPERAKPQYSYKIAVDGKTQIDLEYLLKDMQVTDPAGMIAPDTLFVQ
                     CLTFANAERFKNIPGVTSVKRVINKGPEDGVFPDMKDGKPSVTNNWSGDNYGPIYIPQ
                     EGKTVALNKQSLPFYKIIISEYEKNDLKVVGDDIYINGEKATTYTFKQNYYWMMGDNR
                     HNSLDARYFGYTPEDHIVGKPVFLWMSWDGHAKGFNKIRWERLFTTVNGEGQPQSYFK
                     YFLGLLALYFVGEYFWKKRKEKQE"
     gene            25730..26341
                     /locus_tag="PK218_00130"
     CDS             25730..26341
                     /locus_tag="PK218_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HPW96949.1"
                     /translation="MNIIIHPSYFPSISHYVAMANSDLVTFEMDDNFQKQTNRNRMYI
                     YSPNGIQLLNVPIKHSKTVHQKTKDVQIENDFDWQKQHFKSLEAAYRSSPFFEYFEDT
                     IHPVFEKKHQFLMDLNLQTMEIVSNCLGFEFDYNETTEYFFDVADKTDFRPLVNGKKD
                     VSQLASYTQVFGDKHGFINNLSILDLLFNEGRYAFEYLKQQQL"
     gene            complement(26533..27582)
                     /locus_tag="PK218_00135"
     CDS             complement(26533..27582)
                     /locus_tag="PK218_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPW96950.1"
                     /translation="MKKLSWFNKVMYVLNGILILLTFVAYVLPFLAPKAFPLLSVLTL
                     ILPSFLILNGLFFIYWLLQFKRQLVLSGIVLLIGITFINKFYKLSSTNLPKNEKDFTV
                     MSYNVRLFNLFEWINDKNVGKDILAFVNEKNPDIICIQEYSENAKIDLRVYKYKAIFI
                     EGNKIKTGQAIFSKFPIFNQGNLHLLQDGNNIIFADIKKGKDTLRVYNMHLQSIKISP
                     DVNEISNNVETINKEKSQMVFGRLRDAFKRQEFQTEIFSNHKKDCPYPMIICGDMNNS
                     AFSYIYRNIKGDLNDCFEEAGNGFGQTYNFKYYPARIDYIFADKKMKVKWFKNFSSFN
                     KSDHFPIMTRLSFAD"
     gene            complement(27589..28452)
                     /locus_tag="PK218_00140"
     CDS             complement(27589..28452)
                     /locus_tag="PK218_00140"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPW96951.1"
                     /translation="MSIIEDIKLQYKIGGVANKMIYWNVGIYLLSIILFYQFKTGVFE
                     YPNWLAVSSNPDVLVTRPWTLLTYAFFHYGFLHIFFNMMVLNFTSRLFLTFFTQKQYL
                     GLYFLSAIFASIIFVLSFYLLGKSSLMVGASAAIMALLVASTTYQPLMEVRLLLIGNV
                     KLWHITAVILILDLMQIQLDNIGGHIAHLSGAFFGFLYIKLLQNGTDLSVIVTNTINF
                     FSTIFDKKQKTPFKKVHKTSQRKTPIRESKVVIKDKTQQQIDEILDKISQSGYDSLTK
                     EEKEFLFQSGK"
     gene            complement(28452..29264)
                     /locus_tag="PK218_00145"
     CDS             complement(28452..29264)
                     /locus_tag="PK218_00145"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPW96952.1"
                     /translation="MMRMTETVKQLLIINIIFFIGSQIAPLAYPMFSLYYPENDLFRF
                     WQPLTSMFMHAPMPNLMHIIFNMFGLVMFGSALEHFWGAKKFIFFYISCGLGAALLHL
                     GVSYFQIHSILSELSSLNISQSDMHFLLNTDFSTVFDSSGQMLQGKVKTILDNAQCTQ
                     EQFNTLASAVREFQTPSLGASGAIYGLLTAFAFMFPNAELGIMFIPIPIKAKYFVPGL
                     IAVDLFLGLKGNSLFGSGGTGVAHFAHIGGAITGFIMMWIWKKNQFNKNRWD"
     gene            complement(29261..31108)
                     /gene="mutL"
                     /locus_tag="PK218_00150"
     CDS             complement(29261..31108)
                     /gene="mutL"
                     /locus_tag="PK218_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPW96953.1"
                     /translation="MSSIIQLLPDHVANQIAAGEVVQRPASVVKELLENAVDAKATDI
                     KLIIKDAGKSLIQVIDNGLGMNVTDARLCFERHATSKIRHAEDLFSLHTKGFRGEALA
                     SIAAIAHVELKTKQDQEELGIHIIIEGSKFTTQEPAVLPKGTSFSVKNLFFNIPARRN
                     FLKSDTVEMRYIIDEFQRVALAHPNIAFTMYHNGSESFNLPQSNFRPRIVNVFGGKTN
                     EKLVPVKEDTEIVHIHGFVGKPEFAKKNRGEQFFFVNDRFIKSGYLHHAIMAAYEGLL
                     KDGAQPSYFLYLSVPPNTIDINIHPTKTEIKFDDEHALYAILRSAVKHSLGQFNVAPV
                     LDFERDPNLDTPYQYQQKEAEYPTIQVDRSYNPFADEKPQKAATNFNYKKPELQPSWE
                     SLYVGLEEMDTQSSEISFETDAVSSSLFEDTDTEQETKRTYQIHKKYIVSPIKSGIVI
                     INQKRAHERVLYEEFLTNMTVHHASSQRLLFPLELYFSVNDIEVIKEIKLSLENLGFI
                     FDSIHTDSLIISGLPVTITESQVSIIFDDLLTDFQNGVGGDSFSQNDSIAQSMAKSLA
                     VKTGTYLTEKEQDNLVNNLFACREPNVSPFQKPTFITMSVEDLDKKFSL"
     gene            complement(31459..31956)
                     /gene="ribH"
                     /locus_tag="PK218_00155"
     CDS             complement(31459..31956)
                     /gene="ribH"
                     /locus_tag="PK218_00155"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPW96954.1"
                     /translation="MATANKNLSNYDKSKLPNAKNFRFGIVVSEWNDKITNGLYNGAE
                     AALLDCGALAENIIRWDVPGSFELIYGAQRMIVTQEVDVVITIGCVIKGETMHFEFVC
                     EGVTQGIKDLNVQTDVPVIFCLLTDNTEQQSIDRSGGKHGNKGTEAAIAAIKMAYLRK
                     KTMEM"
     gene            complement(32104..32889)
                     /locus_tag="PK218_00160"
     CDS             complement(32104..32889)
                     /locus_tag="PK218_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW96955.1"
                     /translation="MATFNKRGYRTPKEKADKLDNNYIEDVNVDAKDSATAKAFDTLD
                     ETASRTEDFVQKNQKYILSVLAIIAVGTIAYLLFQKFVAEPKQDDAANELFVAQQNFQ
                     KAVDGDAKADSLFNLVLKGSEGKFGVLKIAEEYSGTDAGNIANYYAGIAYLNTGKYDE
                     AIKSLEQFKADDEVLSTLAIGAIGDAYSQKNQAKEALTYYVKAAEAKKNDLTTPRFLL
                     KAGKTALALGDKAAALKYFTDLKDNYESTPEGQGVDGLIGMAQ"
     gene            33032..34111
                     /gene="recF"
                     /locus_tag="PK218_00165"
     CDS             33032..34111
                     /gene="recF"
                     /locus_tag="PK218_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022322.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPW96956.1"
                     /translation="MFLKNITLFNYKNFSEASFEFDPKINCFVGKNGVGKTNVLDAIY
                     HLSNGKSYFNPLAVQNIKHNEEFFVVDGEFEKNDRTEQIVCSLKKGQKKILKRNGKLY
                     DKLSEHIGLIPLVIISPADRDLITDGSETRRKFMDSVISQLDKSYLQEIIKYQKIILQ
                     RNALLKYFALNHVFEKETLHIYDEQLDDLGQVIFEKRKSFLEKFIPIFNHYYQEISNN
                     SENVQLVYQSDLFEKNMLPLLAENINKDKALQYTSVGIHKDDLSFEIDGFPIKKFGSQ
                     GQQKSFLIALKLAQFDFVKKQSGEKPILLFDDIFDKLDESRVSKIVSMVNQEEFGQLF
                     ISDTHPERTENIVKETHQSYKIFNL"
     gene            34152..34850
                     /locus_tag="PK218_00170"
     CDS             34152..34850
                     /locus_tag="PK218_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPW96957.1"
                     /translation="MKLKIYTYLFISIILSSCQGQHTKKVETLDVKTYAEKLNITNNP
                     QLIDVRTPEEYASGKINNAKNINWNANDFATQAEKLDKSKPVFVYCKVGGRSAQAANK
                     LSEMGFKEIYNLDGGIMKWNAAGMQKEKSTTYIGMTDEAYQKLIASDKKVMINFSAVW
                     CAPCQKMKPYILKMQEELKDHVTIIRLDADENKSLLEKMKIDGLPVVIVYENGKETWR
                     NLGFISEEDLKKHL"
     gene            34891..35565
                     /locus_tag="PK218_00175"
     CDS             34891..35565
                     /locus_tag="PK218_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPW96958.1"
                     /translation="MITKEALQFLADLIANNNTEWMHANKKRYENYKKDYHAFIASIL
                     AEMKPLDKTLEPLEVKNCTFRINRDIRFSKDKSPYKTNMGVWFTQNKNRKNSPGYYIH
                     YEKGNSFIAGGVWCPEASELKQIRKEIEFFHDDLDRIVANKNFKKEFGEITRDENNML
                     KKAPKGYDPEHPAIEFLKMKSFTASAKIDDNLFTQPEFAKTIAEKLITLKPLNDFLMR
                     ALETEE"
     gene            35633..36646
                     /gene="murB"
                     /locus_tag="PK218_00180"
     CDS             35633..36646
                     /gene="murB"
                     /locus_tag="PK218_00180"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPW96959.1"
                     /translation="MQIHTNFSLKNYNTFGIEAKAKQFVAVHSIDELKTVLENHATEK
                     KFILGGGSNMLLTQDIDSLVIHIDLKGKKILKEDDDFVWVESQAGENWHEFVLWTIDQ
                     NFGGLENMSLIPGNVGTTPVQNIGAYGAEIKDTFVSCEAMEITTQKMKSFSKEECRFG
                     YRESIFKHEVKDKYIITSVIFKLTNRNHKISVEYGDIKAELAKNNIEKPSLKDVSNAV
                     ITIRNSKLPNPKELGNSGSFFKNPILSREQFLKVQEQFPDVKHFDISETEVKVPAGWL
                     IEHSGLKGYRKDDAGVHKNQALVLVNYGKATGQDILSLSKYVQKTVFDKYGIAIEAEV
                     NVI"
     gene            36682..37185
                     /locus_tag="PK218_00185"
     CDS             36682..37185
                     /locus_tag="PK218_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW96960.1"
                     /translation="MKKILETSRTYLRELSTDDAENFFLLNSDKDVLKYTGDKPFESI
                     LKTKEFLKNYNPYQEFGYGRWAVIDKSNNEFLGWCGLKFTRELNEVDLGFRFFKKHWN
                     KGYASETAKACVDYGFEKLNLTKIVGRAMKENSASIKVLEKAGMQFVDIIDFEKHEGV
                     LYQLKKQ"
     gene            37186..37797
                     /locus_tag="PK218_00190"
     CDS             37186..37797
                     /locus_tag="PK218_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HPW96961.1"
                     /translation="MYIPEIYLNENQAEIEQFIHENGFGILINQTKGKLWATHIPLLL
                     ENKNDKQILVGHISKLNPQADSFKTNDDVLAIFSGAHSYISSSWYDHENVPTWNYLAV
                     HVYGKVKLHSYEQSVEALKSLVDNYELKSKNPVKVENLSEKTMREAHGIVSFEIEITR
                     IESVKKLSQNRDNKNYKNIISELEKTNENQSIEVAKAMKKNRP"
     gene            37874..38980
                     /locus_tag="PK218_00195"
     CDS             37874..38980
                     /locus_tag="PK218_00195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW96962.1"
                     /translation="MLLSLFCLFAAVVLIQFFYYIIVFGKFSFGKPQNETPKKIPISV
                     IVCAKNEEDNVRNFIPLLAEQNYPQFEIVLIDDASSDTTLDIFEEFEKQYSNVKLVKV
                     ENNEAFWGNKKFALTLGIKAAKYEYLLFTDADCYPTSKDWITKMSSQFTQQKNIVLGY
                     GAYEKIANSFLNKLIRFETLLSSTQIFGWTKLGKPYAGVGRNMAYKREEFFKVNGFID
                     HMKIRSGDDDLFINQAANKNNTTICYLPDSFTYSKPKTTFSEWFTQKRRHVSTAGHYK
                     GFDKIQLGLFYSSQLLFILLPIVLLAFQYQWIAVVSMIGFRYIFTWISMGFSAGKLKE
                     KDVIYWFPVIEIILIFVQLNVFISNTFKKPVHWK"
     gene            38971..39519
                     /locus_tag="PK218_00200"
     CDS             38971..39519
                     /locus_tag="PK218_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW96963.1"
                     /translation="MEIKQYIENAKNGDQVAFTFLLDNYWNEVYGFMLKRTENETDSE
                     DITIETFSKAFDKIATYNPEFQFNTWLIAIAKNVHIDMLRKKKSSLFVDISENDDRQA
                     YNIADSSPTAEDELITEQNLSQLLRYIKELKPHYQEVIQLRYFQEMSYQEISEQLDEP
                     LSNVKIKLLRAKKLLAEIIEKK"
     gene            39630..40475
                     /locus_tag="PK218_00205"
     CDS             39630..40475
                     /locus_tag="PK218_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW96964.1"
                     /translation="MSNVSIYGKKWIDLVFEDRNQMYGAYQLRQQNPKMTLLALFYGV
                     LLMAGFMSIALYSNFRSEEISITDIIPEETIIQLSNYSEPVPEVETVVKTTPPNTVTE
                     PIVEPEINRFSHLTVSQSSEAVVKVPTNEELNTVETPSIIGTTAIVGSGIEPGTDSGK
                     TNGNLLGNEGNNSTNNTPSKASEVDKLPAFPNGIENFYKYVASNFRNPDLESGAIVSV
                     LVAFVVEKDGTLSNIRVLRNSGFGVDKEAIRVLSALKTKWSPGIKNGEKVRTEFTLPI
                     KVKFN"
     gene            40610..41782
                     /locus_tag="PK218_00210"
     CDS             40610..41782
                     /locus_tag="PK218_00210"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW96965.1"
                     /translation="MQMKFLTVTFFSIVLLYFSSPKQHSFVLKSEEIPSDSSKISIPY
                     FLKLGKVSDSFIAEKSKKIDSFYIKNIGTAGYYGSFIVAKNGKIIYEKYQGMANLKLQ
                     KPMDENSALHLASVSKVLTATAILKLVENDNIMLDQKVTDWLPSFPYKSTTIRTLLNH
                     RSGLQHYSRFPELMKKNWNSKKILSNKDILELMVKNKFRLISPDNTRFDYCNTNYVML
                     ALIIEKATGLSYGEAMKMLIFKPLNMKNTFVFDYFRDKDTVSMSYRNNKANPWDQFDN
                     LNGDKNIYSTPRDLVKFDLATYSNDFINQNLMKEAYVGYTSNMKLGKDYGLGMRMKKL
                     ANGETQVYHNGWWHGNTSSFVPVKKDTISIICLSNKYSNNTYETLKLVESLYPQTK"
     gene            complement(41770..42594)
                     /locus_tag="PK218_00215"
     CDS             complement(41770..42594)
                     /locus_tag="PK218_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HPW96966.1"
                     /translation="MKLGEFNTLIIDRLTKVGLFLTDNTNDVLLPLKYIPKDYKIGDE
                     IKVFLYLDHEERPVATTLEPYIIINDFAFLRVNYTNSFGAFLDWGLEKDLFVPFKEQA
                     RPMEQGKRYLVHAYLDDKSDRIVGSSRVNKFLDNSELDLQQGDEVDLIISHITDLGTN
                     VIINKKHKGLMYSNEIYEDLRIGDKHKGYIKTIRPDNKIDVSFQKIGVENIEPNAEVI
                     LKELKANRGFLRLNDNSHPEDIKTVLKMSKKSFKKAVGSLYKDKIIEIKEDGIYLV"
     gene            42724..43218
                     /locus_tag="PK218_00220"
     CDS             42724..43218
                     /locus_tag="PK218_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW96967.1"
                     /translation="MSETLEQSFVQQLKENQNIVHKICRLYTNDSDAHNDLFQEITIQ
                     LWKAFPKFRGDSKFSTWAYRVALNTAITLYRKSTRSVSTVSYEVQKHFFAHEEYNYEE
                     EEKLKLMYQAVHLLNDIEKALVYMYLEDKDYSEIAETLGISEVNARVKMNRIKGKLIK
                     ILNP"
     gene            43225..43863
                     /locus_tag="PK218_00225"
     CDS             43225..43863
                     /locus_tag="PK218_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW96968.1"
                     /translation="MNELDLLKKDWKKENPNYTQVSEFDIYKMLHKNSSSTVKWILII
                     GIVEFIVWTGIGFFFNTDDYVKSIHAEEMMIYLKVLTYLNYAVVLWFIYKFYMNYKNI
                     STMVSTKQLMKDIMKTRKTVNQYVAYNLAMIVVSMILGFIMAFLYNPKLSVIKEKIAN
                     DASNGVLLKIIGLLTLAIVIFVVIFWLFYKLLYGILLKRLKTNFKELQKIEI"
     gene            43865..44719
                     /locus_tag="PK218_00230"
     CDS             43865..44719
                     /locus_tag="PK218_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPW96969.1"
                     /translation="MKKIALLFGLFLALFSNAQTEKKSLLWKISGNGLAKESYLFGTI
                     HIACDATLPKKVLDALDKTNQLCLELDMDDPNMQTEMMGKMMMKNGVTIESMMSEDDF
                     KLVDDFFKKQSGFSLKMMNSIKPFALSAMLYPKMLDCPIQSFENELMKVAKEQNEETI
                     GLETIGEQLAIFDAIPYQTQVNELIKSAKSDLSTDKKELEALLELYKSEDLEAMLQYS
                     DSSENIMTSEFKEELLNNRNKNWIDRITKIAMEKPTFFGVGAAHLGGENGVIKLLRKK
                     GYKVIAVY"
     gene            complement(44716..45540)
                     /locus_tag="PK218_00235"
     CDS             complement(44716..45540)
                     /locus_tag="PK218_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW96970.1"
                     /translation="MGLFKRNPFGHILFIKKWLIRIFGALTHRRYRGFNQLQIEGSEI
                     ITSLPDKNVLFISNHQTYFADVVAMFHVFNASLSGRVDTIKNVGYLWQPKMNIYYVAA
                     SETMKSGILPRIMAYVGAITVERTWRAGGKDVEEKKEVNPNDTENIKIALDDGWVITF
                     PQGTTKSFKPVRKGTAHIIKQHKPIVVPIVIDGFRRAFDKKGLRVKKKNILQSFIIKE
                     PLEIDYENDSIDAIVEKIEFAIEQHPSFLKVIPAEEIEEQERLNKLRKWQPKKTID"
     gene            complement(45587..46225)
                     /locus_tag="PK218_00240"
     CDS             complement(45587..46225)
                     /locus_tag="PK218_00240"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPW96971.1"
                     /translation="MDFDNFLQFTPKILNVGLPATIAHAKMTPSNRMDLLKKLNPNNV
                     NPKKAAVMMLFYPKNNQTHLVLILRNSYKGVHSSQIAFPGGKPELIDVDLKETALRET
                     HEEIGILPNKIEVIRSFTEVYIPPSNYMVHPYLGISREELNFVRQEEEVAGIIELPLV
                     DFLDERIIQNKKMNTSYASNFEVPGFLVKEHFVWGATAMMLSELKETLKIVL"
     gene            46291..46734
                     /locus_tag="PK218_00245"
     CDS             46291..46734
                     /locus_tag="PK218_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HPW96972.1"
                     /translation="MYSKEEAQKIKRQFWIAFAVAYPRKWMLYETKIKDVSFKFFVDN
                     KKAQVMLDIEPKEEEKRKIYFEKIESLQSILLEDHLPEAILERNFHLETGKTISRVWV
                     EIDNVSINNQKTWETIFAFFNEKMSAFEYFFLEYEDYIKDLETNT"
     gene            complement(46731..47027)
                     /locus_tag="PK218_00250"
     CDS             complement(46731..47027)
                     /locus_tag="PK218_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPW96973.1"
                     /translation="MAKRISNESSISDVLKEFIEVNKLQSGMDKIDVQQAWKSLMGNG
                     VNSYTNDVILKGSTLYVSLSSAVLRQELSYGKQKIISMINEELRKDVVKDLIFR"
     gene            complement(47027..47443)
                     /locus_tag="PK218_00255"
     CDS             complement(47027..47443)
                     /locus_tag="PK218_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW96974.1"
                     /translation="MKRIIVLVIAFLFVSCQKEIKKEDLINLNGYWEIKQVTKPDGEK
                     KEYKVNETVDYFEVKNTIGFRQKVMPQFDGKFKTNEIKESIKIIDSNKTFFIEYTTDY
                     GKWKEEILSLKDSTLVLKNKENLEYTYKKFKPFSLK"
     gene            47580..48653
                     /locus_tag="PK218_00260"
     CDS             47580..48653
                     /locus_tag="PK218_00260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPW96975.1"
                     /translation="MKKLYILLLFPLLLSNCSADSNENPVEEQMYFPPTDGSTTWETK
                     SILSLGWNQNAVQPLLEYLELKHSKSFMIIVNGRIVMENYFNGHTATTNWYWASAGKT
                     LTATLSGIAEQEGFIDINNKVSDYIGTGWTNAPLAKENLITCKNLLSMNSGLNDALGD
                     DVSPTNLQYTADAGTRWAYHNVYVKMQDVVAQSSGQTWNNYFNTKLRDKIGMNGFWFN
                     SGTSGDLSVYWSTTRSMARFGLLNLNKGKWNGTQILNENYFAQATTTSQNINLAYGYL
                     WWLNGKTSYHLPQTQIQFSGSIIPNGANDMFMALGKNDQKIYVIPSKKMVVIRMGDAA
                     DDENFTLSNFDNELWGKINALIL"
     gene            complement(48848..49828)
                     /gene="ftsY"
                     /locus_tag="PK218_00265"
     CDS             complement(48848..49828)
                     /gene="ftsY"
                     /locus_tag="PK218_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPW96976.1"
                     /translation="MSFFKRLFSSEKNDPSKSVEAKQNLDKGLEQSKSSFFSKLTKAV
                     AGKSKVDDEVLDNLEEILVSSDVGVDTTLKIIQRIEKRVANDKYLGTDELNKILREEI
                     AALLSETNSGDETQFSIPKDTKPYVIMVVGVNGVGKTTTIGKLAHQFKNAGYSVVLGA
                     ADTFRAAAIDQLQIWADRVGVPIVKQQMGSDPASVAFDTLQSAVSQNADVVIIDTAGR
                     LHNKIGLMNELSKVKKVMQKVVGDAPNDVMLVLDGSTGQNAFEQAKQFTAATDVTSLA
                     ITKLDGTAKGGVVIGISDQFQIPVKYIGVGEGIEHLQVFNKYEFVDSFFK"
     gene            complement(49925..50077)
                     /locus_tag="PK218_00270"
     CDS             complement(49925..50077)
                     /locus_tag="PK218_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HPW96977.1"
                     /translation="MAKKTVATLQTTSKRLTKAIKMVRSPKTGAYIFQESIMAPELVD
                     EFLTKK"
     gene            complement(50123..50305)
                     /gene="rpmG"
                     /locus_tag="PK218_00275"
     CDS             complement(50123..50305)
                     /gene="rpmG"
                     /locus_tag="PK218_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPW96978.1"
                     /translation="MAKKGNRIQVILECTEHKESGLPGTSRYITNKNKKNTPDRLEIK
                     KFNPILKRMTVHKEIK"
     gene            complement(50334..50570)
                     /gene="rpmB"
                     /locus_tag="PK218_00280"
     CDS             complement(50334..50570)
                     /gene="rpmB"
                     /locus_tag="PK218_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPW96979.1"
                     /translation="MSRVCDLTGKKAMVGNNVSHAMNKTKRKFSVNLVKKRFYLAEED
                     RWISLRVAASTIKTINKKGLSAVLKDAKANGFIK"
     gene            complement(50646..51896)
                     /locus_tag="PK218_00285"
     CDS             complement(50646..51896)
                     /locus_tag="PK218_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HPW96980.1"
                     /translation="MKATIVTIGDEILIGQIIDSNSSYIAKSLDKIGVQTHEMLSISD
                     DKKHILDTFLNLQNKVDFVIITGGLGPTKDDITKKTFCDYFEDELVENVQVFNHVKEL
                     IEGFYKRPITQLNRDQALVPSKCEVLFNKMGTAPGMWMKKDKTVFISLPGVPYEMKYL
                     IENEIVPKIVKEYKRPFILHKTILTYGQGESLVAERIENWENNLPEFIKLAYLPSPGR
                     VRLRLTARGERKEILQKGIEENVNTLTKIINDIIVGFDEDETIEVVLGKLLTQQKRTL
                     ATAESCTGGKIAQMITSVSGASNYFRGGVVTYATDTKITQLNVNPETIKKHTVVSAAV
                     AKEMVIGIQKTMNSDYAIATTGNAGPSKGDSEAEIGTVFIAISTPKKVIVEEFNFGQP
                     REKVIDRAVNKSFELLQKEILKNV"
     gene            52009..52614
                     /locus_tag="PK218_00290"
     CDS             52009..52614
                     /locus_tag="PK218_00290"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPW96981.1"
                     /translation="MTFNPEELDKSAAYKLLTGTIIPRPIGWISTVDENGNNNLAPFS
                     YFNMVSSDPPCVMFSTRRDNNQNKDTLNNVLITNEFVVNLVTVDIVEKMNTTSASVPP
                     DIDEFELAKLTPIDSVMVKPKRVKESLVHFECEKIHHYFIDNDSNGGACVVIGKIKII
                     HIDDTILLENNYINLEKYKPVARLAGSNYGTLGEIFSIKRA"
     gene            52616..53086
                     /locus_tag="PK218_00295"
     CDS             52616..53086
                     /locus_tag="PK218_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPW96982.1"
                     /translation="MKTNELIIDVAMQIQKPSNEVFEAIVNPEKMTNYFISESTGMME
                     EGKELIWKFPEFDFDCPVRIGKIEKDKYVSYYWMMEEKELFVEITLTEKENNSTLVSI
                     TEKSMNNDEKGLKWLSGNSFGWSNFLSCLKAYLEYGINLRKGAFDFMRKENQCS"
     gene            53097..55388
                     /locus_tag="PK218_00300"
     CDS             53097..55388
                     /locus_tag="PK218_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPW96983.1"
                     /translation="MKITVIGGGPGGLYFSILTKKAMPDCQIDVYERNRPDDSFGFGV
                     VFSDETLGEFLKKDMKSYELIRSKFAYWDDIIIARDGQEVSIAGNGFCGCSRKTLLQL
                     LHQRCEEEGVNLHFEANVDDLSQFSDSDVIVACDGISSQIRTQFESDFGTKVELKKNK
                     FVWMGSTKPLDAFTYFFKETKLGAFAAHTYQYEKGMSTWIFECAPETWEKFGFDTFNE
                     EDTVQKLQELFKTELDGHGLITNKSHWRQFPHVTNENWYHNNIVLLGDAKATAHYSIG
                     SGTKLAMESAIALSDAIIANPNDIQTAFQNYDKSRRNSVEMIQYAANVSLDWFENLDR
                     HIQHPFYQFAFGCMTRSKKVTYENLQIRDKSFTDKVLHEFNDNYNVNDKYQSAAFSTF
                     KLRDLVLQNRIVMSPMGQYSAENGLVNDWHFQHYASRAIGGLGLILTEMTAVSEKGRI
                     TLGCAGIYDENQIVEWKRINDFIHATTQSKIGIQLGHSGRKGATKKPWETEDLKDKWE
                     LLSASAIPFNENFSIPKEMTLEDMDLITSQFVQATKNADKAGFDMIELQAHHGFLLAS
                     FLSPLTNNRKDEFGGSIENRLKFPLRVFNEMRNTFPKEKPMSVRISATDWAENGISEE
                     DVLIIATAFKNAGADIINVSTGNTVANQKPQTGRMWQTPFSDTIRNTVNVPTITTGYI
                     QDIDQINTIILNGRADLVALGRPLLLDTNFVRNAQAYEKYQTDDIPNPYKMGISHLYP
                     LKASERKQTEGMKKALKPKSNKK"
     gene            55467..57059
                     /locus_tag="PK218_00305"
     CDS             55467..57059
                     /locus_tag="PK218_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW96984.1"
                     /translation="MKHYTDNFAHDNLPSIDLQPDYLCDLPQFQHPEMLNCVERLLDN
                     HIKKGNGDAVCIRTFEESWTYNNLFEKANQIAHVFIDDLGLVSGNRVLLRSANNPMMV
                     ACWFAVLKAGGIVVSTMPLLRSKELTTIIDCAEISIAISDSDLADEMNQVESKFLKIT
                     SFFRNGDLDKLTQSKPKNFENYHSKADSVCLIGFTSGTTGLPKMTAHYHKDILNICEA
                     FPVYSLQPTKDDIFIGSPPLGFTFGLGGLVLFPMYFGASTFLIEKPSPDLLLKAIQEY
                     KVTICFTAPTAWRVLTTKLNEFNVSSLRKCVSAGETLPLQVWHDWYDATGLKIIDGIG
                     STEILHIFISSNEENMIPGATGLPILGYEAKIIDENGNELPINQSGKLAIKGITGCKY
                     LNREEKQKEYVQNGWNITGDIFKKDDNGYYWFVARGDDMIISSGYNIAAIEVESVLLT
                     HEDILECAVVGLPDEERGMLVCAHIVLKDNNKASVEFKKTIQQWFKQVAAPYKYPRVI
                     NFIESLPKTETGKIQRFKLKKH"
     gene            57111..58691
                     /locus_tag="PK218_00310"
     CDS             57111..58691
                     /locus_tag="PK218_00310"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HPW96985.1"
                     /translation="MRKTFLVISFTLLSLTAILTYINWKFSFTLLILIPLILMGLYDM
                     YFADRTIRRNFPLLGRMRYILSAIGPEMRQYFIETDLDGKPFNRLQRDIVYHRSKKEN
                     DTMPFGTQLDVYRDGYEWINHSISAIPFAKVNHNPKVKIGSSQCTKPYDASLFNISAM
                     SFGSLSKNAIIALNKGAKQGGFYHNTGEGGLSPYHLLGGDVVWNIGTGYFSCRKFDGT
                     FNVDEFEKRAILDNVKMIEIKFSQGAKPGHGGILPKEKVTAEIAAIRLVETGHDIVSP
                     PSHSAFTTPLELMDFVKLLREKSGGKPIGMKICIGNKSEFLSICKAMVQTKTYLDFVT
                     VDGGEGGTGAAPQEYSDHVGMPLRDALAFVYDALNGFGIKDQIKIIASGKVVTGFDII
                     RNLSLGADLCNSARGMMFALGCIQALECHLNTCPTGVATQNPNLMKGLVPEDKSVRVT
                     RFQQETVKSAIDLMASAGILHPDYVTRDVITTRIDRNKVETFAQTFPELETGCLLNEN
                     TVPEKFLYFWKRASVEKF"
     gene            58701..59141
                     /locus_tag="PK218_00315"
     CDS             58701..59141
                     /locus_tag="PK218_00315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPW96986.1"
                     /translation="MNNYFIKQQKIRFKHVDYAGIVFYPRFLEMLNDLVEDWFEEALN
                     RPFSKMHDATNGESYGIPTVDLKVQFKTPARIGEVITKKLWVTQLKTSSLLCGFQFVN
                     QDDKTVLEGEVTLVNVKIEKEKDAIKSEAFNEEMKSKISTFIQS"
     gene            59172..60152
                     /locus_tag="PK218_00320"
     CDS             59172..60152
                     /locus_tag="PK218_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPW96987.1"
                     /translation="MDFKKFKAATVQTSPVFLDVEKTIEKAISFIKEASQNGATLIAF
                     PEVFVAGYPYWNWIMTPVQGSKWYEKLYKCSLKTDDESMKPLFQAAKDFNIHIVIGIN
                     ERGNSYGEIYNTNLIIDNNGKLIGKHRKLVPTWAEKLTWSSGDGSSLKVYDTEVGPIG
                     TLACGENTNTLARFTLLSQGELVHIANYISLPVAPPDYNMAEAIKIRAAAHSFEGKLF
                     TIVSCSTISKEIMNAMKDDVPDIEELLTRKNSAFSGFIGPNGAVIGEPLIDDEGIIYA
                     NIDLEKCIQPKQMHDILGHYNRFDIFDLRVNTAPTRKITFIDNHEEFNKK"
     gene            complement(60466..63948)
                     /locus_tag="PK218_00325"
     CDS             complement(60466..63948)
                     /locus_tag="PK218_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW96988.1"
                     /translation="MKKKLLLFTFLIIPVLSIHSQSVGDTIKVRTFNFNSTTRDTLIN
                     FPNDPGLTFEKIILKYNMRCKDGLVSTGTQRDLGCGEWDYSCNTFIADDSKIEKVLFK
                     QPDYVVSNFAGTTFNYTSQQTYNHYDFTQKNVIVNNIINENNYVVGANNTSVSNVLNT
                     NLKSGRTQILIKASELLNSGFSSGLINALSLNVTNAGGLTKFLKVKIKNTNLTQLQAQ
                     NIDFNGFTEVFFSDYTFVAGTNKLQFHTPYNWDGTSNIIVELSFSNTTTSTPIEFSGF
                     TNVETRTLTANNIAAIDVSSNGLVDINAQFFNTINNEVTVTFWTKGDENLMPVNNSIV
                     YAFGENISQRQLNLHIPWGSSVYFDCGNVNGSYDRIEKPYATDNVIKGVWNHWAFTKN
                     ATTGVMNIYLNGVLWHTGTSKTNVITILNLVLGKFDDGGTLAGYKGKIREFTIWNKAL
                     SATSILNWKNRTIDATHPDYSNLLAYYKLDELTGQVVNDSKNGLTSNGINLNWDYERG
                     DQLSTTFIESSTVPTITLYRGNYDQTVADVIVRYSSPKNANVVNHYSITSNEGVTPMQ
                     HDAINLLSSVNLFEATPENVYDGDTGNIIATIPVPVEGTINAVDLDYYKRFPFYNELV
                     SFVTPYGIGLDLGPNGKSWYFDMSDYVKILKGNKRIVMNGGVWQEDLDLEFWFIVGTP
                     PRNVVQYDQLWQGSYRLGSPNINEIINGTKMPTVNYDFSAQATAFKLKSSITGHGSQG
                     EFSQNGGTVSHKILVNNVQKFSWSITQDCSENPIFPQGGTWVYNRQGWCPGQRTLLKE
                     QNLTPHVTPGTTMAIDYRTSNPQVPTGDYRYIVAHQVVGYGPANFALDAAITEIKMPN
                     NKNAEFSRINPMCERPKITLSNTGETVLTNVEIEYWLNSASTHQTYTWTGTLASMASV
                     DVFLPTNALWNNGVEAANNKFHAQIMTVNNGADMYSNNNHLISSFTLPDVMPTVFKIQ
                     LKTNNSPTQNNYSLYDSNGTLIETKTFPTANTVYTYTYQEPQIGDGCYRLRVNDSGKD
                     GLQWWANTSQGTGYMRLLDANDVIIKTFNPDFGGGFDYSFSVNSLLSNTVLATDNDIK
                     VYPNPSYGNFSVAGKELNGATITVIDILGNVVVQQLADNDAVNFNSIQLSTGVYFVKI
                     QKDKNTVTKKLIIN"
     gene            complement(64021..65655)
                     /locus_tag="PK218_00330"
     CDS             complement(64021..65655)
                     /locus_tag="PK218_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPW96989.1"
                     /translation="MITVNDISVHFGGTTLFSDVSFAINENDKIALMGKNGAGKSTLL
                     KIIAGEAKPSTGNISAPKDAVVAYLPQHLLTKDGSTVMEEASKAFSEIFKMKADIDDI
                     NEQLTIRTDYESDEYMKLIERVSDLSEKFYAIEEINYEAEIEKILKGLGFTAEDFKRQ
                     TSEFSGGWRMRIELAKILLQKPDLILLDEPTNHMDIESIQWLEDFLINSAKAVVVISH
                     DRAFVDNITNRTIEVTMGRIYDYKAKYSHYLELRKERRIHQQKAYDEQQKMIAENQEF
                     IDRFKGTFSKTLSVQSRVKMLEKIVPVEIDEIDNSALKLKFPPSIRSGQYPVVVKELE
                     KSYGDKLIFKDANIVIERGDKVAFVGKNGEGKSTMIKAIMKEIEINGGSVEVGHNAMI
                     GYFAQNQASLLDENMTIFETIDSIAVGDVRTQIKNILGAFMFHGDDVTKKVKVLSGGE
                     KTRLAMIKLLLEPVNLLILDEPSNHLDMKTKDIIKDALRDFDGTLILVSHDRDFLDGL
                     ATKVFEFGNKRVKEHFEDIKGFLAHKKMENLREIEK"
     gene            complement(65789..68803)
                     /locus_tag="PK218_00335"
     CDS             complement(65789..68803)
                     /locus_tag="PK218_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPW96990.1"
                     /translation="MSKLVYRIVIVVVFFIFVNCSINKQVTKVPTVPKNQEIEFPEVT
                     IDEEVYIPRIKNERRDFFPKTEGETVWVDSIYNQMAFEEKVGQLFMVAAYSNKDAAHS
                     AEIDKLVSDYKIGGLIFFQGGPGRQARLTNRYQAKAKVPLFIGIDAEWGLSMRLDSTY
                     RYPWNMTLGAIQDLKLIEKVGENMGKESKRLGIHFNFSPVLDINTNPKNPIIGFRSFG
                     ENKKNVSEKAIALMKGIQGNGVYTTGKHFPGHGDTSTDSHYALPLVTASKEHIEKIEL
                     YPYKRMFDEGMVSVMVAHLSVPSLESDANVPTSLSKNVVTDLLKKELGFDGLIFTDAL
                     NMKAASNFKKPGDIDLEAFLAGNDVLLFSENVPVAVEKLRTAYQDGVITEERLAESVK
                     KILKYKYKIGLNAYEPVELINLYNELNMPAKNALTYQLYENAVTVIKNDDELLPIKKL
                     DQKIAYIKLGDDDNTTFVSTLKKYTEITEIKETNLDSIKIQLKDFGTVIIGFHKSDKA
                     WKNHDFNLQEIQLIEGISKQNKVILDVFAKPYSMLQIPNFDNITAVVASYQNNPIAQE
                     VSAEIIFGALGAKGKLPVSITNQFRAGSGIKTESINRLGFTLPENVGMNATKLAQIDK
                     IANKAIDAKMTPGIQVLVARKGKVIYQKSFGYQTYAKQVKVKNTDLYDVASLTKMVAT
                     LPNVMQVYDKHKVNLETTLGEMLREFKNSNKENITFKELLSHYAQLQAWIPFYKETLD
                     ANGKPSEKYYRKTYSKEFSKQVCENLFIRNDYEDTIMKKIIDSPLLDKKEYKYSDFTF
                     IILKKYLEKVTGKSLDELTYTNFYKSMGMNNTMFNPLRKFDASLIAPTEIDTYYRYTT
                     LQGYVHDMEAAMEGGIGGHAGIFSNSLDLAKMMQMYLQKGTYGDKEYFSERTFNDFNT
                     CYFCESGNRRGLGFDKPQLGTSGPTCGCVAMSSFGHTGFTGTMAWADPETDIIYIFLS
                     NRTFPDSNASNRLSKENIREDIQKIIQEAIVE"
     gene            complement(68818..69300)
                     /locus_tag="PK218_00340"
     CDS             complement(68818..69300)
                     /locus_tag="PK218_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HPW96991.1"
                     /translation="MFVPFDTLPEYSKIWIYQSNRKFSDEEITEIENDLKTFVENWSA
                     HGTGLEASFILKYNRFIILSVNQEVQQATGCSIDSSVAFIQNLEQKYKVDLLDKMNVT
                     FKNGEHIAHKSLIDFKRMAKEKAVTANTIVFNNLVNTIEEFNNNWEVPAEESWHSRFF
                     "
     gene            complement(69419..70210)
                     /locus_tag="PK218_00345"
     CDS             complement(69419..70210)
                     /locus_tag="PK218_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPW96992.1"
                     /translation="MSEIINVPTMAEMLAQGKQPEVLFWVGCAGSFDDRAKKITKAFV
                     KILNRAKVEFAVLGTEESCTGDPAKRAGNEFLFQMQAMMNIEVLNAYEAKKIVTACPH
                     CFNTLKNEYPELGGKYEVVHHTEFLKSLLDTGRLTIEGGQFKGKRITFHDPCYLGRAN
                     NIYEAPRDLIQKLDAELVEMKRSRSNGLCCGAGGAQMFKDAEPGNKEVNVERTEDALE
                     TKPDIIAAGCPFCNTMMTDGVKNKEKEATVKVMDIAELIANAQDL"
     gene            complement(70277..70723)
                     /locus_tag="PK218_00350"
     CDS             complement(70277..70723)
                     /locus_tag="PK218_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HPW96993.1"
                     /translation="MNTEDIDKNLQTISENGKLLSPILPEGIKNYLIDIDGTICDDIP
                     NEEPERMLTAEVYPDALITLNKWYDEGHIIFFFTSRTETHREYTERWLKQHGFKYHGM
                     LMGKPRGGNYHWIDNHLVKATRYRGKFTDLVEKEVTIQVFDDEHHE"
     gene            complement(70807..72114)
                     /locus_tag="PK218_00355"
     CDS             complement(70807..72114)
                     /locus_tag="PK218_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPW96994.1"
                     /translation="MEYIDNLLFALILIAGIGYFTINVKKLVRNINLGRDVNRSDNSS
                     ERWKNMAMIALGQSKMIKRPIAGFLHIIVYVGFVIINIEVLEIIIDGLFGTHRVFSFM
                     GSFYGFLIGSFEILAVLVLVAVIVFWIRRNVVKLKRFMNSEMKTWPKNDANYILYFEM
                     VLMSLFLIMNATDVHFQEMNSGNIISSHIAYWFGGFDVSQLHLIERTAWWIHICGILV
                     FLNYLYFSKHLHILLAFPNTYFADLNPKGQFDNLESVTKEVKLMMDPNADPFAAQPVD
                     ENAVPSKFGASDVQDLNWVQLLNAYTCTECGRCTSVCPANITGKKLSPRKIMMDTRDR
                     LEEVGKNIDANKGVFIPDNKSLLNDYITTEELWACTSCNACVEECPVNISPLSIIMDM
                     RRYLVMEQSAAPQSLNAMMTNIENNGAPWQYNQMDRLNWKDEN"
     gene            complement(72127..73080)
                     /locus_tag="PK218_00360"
     CDS             complement(72127..73080)
                     /locus_tag="PK218_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPW96995.1"
                     /translation="MKITREIKTAILVIASILLFIWGYSFLKGKDLLSNYTTFYVKYD
                     NVDGLVRSAPVTINGLTVGKVSSIKLNEDWTSVVELQINGDYKITKNSIAELYSPGPI
                     GGKQVAILPNKETKDIAKSGEYLVSSSKLGLTEEVSKEIKPIKDKLNSVLENANTMLI
                     NINQVLDAKTKENLKNSLENLNATLAQFKEVSQNANEMLADNKTKISATMTNVENASN
                     NFSKMSDSLAKANIGETVKKLENTLASVDKLMADIQSGKGTLGKLAKDDAMYNNFTKA
                     SKELELLLQDLRLNPTRYINVSLFGKKNKPYQAPSNDSISK"
     gene            complement(73131..74303)
                     /locus_tag="PK218_00365"
     CDS             complement(73131..74303)
                     /locus_tag="PK218_00365"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPW96996.1"
                     /translation="MIKEQKLNIGIVATIFLLVTNFMFSQSNQKFKVTLDAGHGDHDF
                     GAVYDGRIEKKIALAVVLKVGKLLEKNSDIDVIYTRKTDVFVDLIERANIANRADANI
                     FVSIHCNANKNREASGCETYVMGLSKNASNLEAAKKENQVITLEKDYQQKYKGFDPKS
                     PESMLGLIVENEEYLNNSIELAGSIQKEFINVGKKSRGVKQAPFMVLHKAYMPRVLIE
                     MGFISNPAEGSRLDSEDGQDEIAESIAKAIIRYKKEYYGTYGNESDEKPSDKVVPVVI
                     DSKPASTSTTPTTTPKTDEKPKETPKPATKGVVFKVQISASSTKLELIPSNFKGLSPI
                     SMIETNKFYKYMYGETTSYADAKVLLQQAKATGFESAFVIALRDGVRIDIGEALKQ"
     gene            complement(74325..75368)
                     /locus_tag="PK218_00370"
     CDS             complement(74325..75368)
                     /locus_tag="PK218_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPW96997.1"
                     /translation="MTAFAQSKSGKFVVVLDAGHGGKDPGNSYHGYVEKDIALKTTLK
                     VGDFLKDEKDFEVVYTRKSDVFIELVNRPKVANKINANLFVSIHCNSVKNFEPAGTET
                     FVMGLSRTDMNMEVAKNENSVIFLEDNYKKTYEGFDPHKPETFLGLKIIQEENLNNSI
                     NLASGIQDNFTSNLNRKTRGVKQQPLWVLDAAYMPGVLIELGFLSNKEEGEFLNSDEG
                     QTKMARQIADAIISYKRENFNELVLANDTKKSARKEIIEDKIEIDSSKSSSKDKEDKN
                     SSGKYKVQLFVTSRKRDTRSSDFKGLRKITYIYENKLYKYYFGSASNEDDARKLCDEA
                     KDKGFTDAFIVSF"
     gene            75510..78232
                     /locus_tag="PK218_00375"
                     /pseudo
     CDS             75510..78232
                     /locus_tag="PK218_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
     gene            78272..78652
                     /locus_tag="PK218_00380"
     CDS             78272..78652
                     /locus_tag="PK218_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPW96998.1"
                     /translation="MKKIIFTAKAPAPIGPYNQAVLIKDTLYTSGQIALHPETMELIL
                     DDIETETKQVMENMKAVLEAAEMTFENVVKATIFIMNMGDFARINSVYGSYFDEKTAP
                     ARETVQVACLPKNVNVEISMIAVK"
     gene            79071..79550
                     /locus_tag="PK218_00385"
     CDS             79071..79550
                     /locus_tag="PK218_00385"
                     /inference="COORDINATES: protein motif:HMM:NF017666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HPW96999.1"
                     /translation="MNTSFERSENTKVEWLTPPELVKCLGVFDLDPCTPINTPFFHAK
                     NNYTTLDDGLKKEWFGRVYLNPPYGRGMELWLEKLKFHGNGIALIFARTETKCFFEHI
                     WNDADAVLFVKGRIRFYHVSGVQGGTPGSPSVFIAYGKENANTLKTCGIEGRFLNLK"
     gene            complement(79569..79818)
                     /locus_tag="PK218_00390"
                     /pseudo
     CDS             complement(79569..79818)
                     /locus_tag="PK218_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024253.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(79920..80123)
                     /locus_tag="PK218_00395"
     CDS             complement(79920..80123)
                     /locus_tag="PK218_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97000.1"
                     /translation="MKKYIFISPEGTTEAPNSTYEVNNMQVIGIVENVDNEDQALIKL
                     LIENEWIIDAEYNIAEFICYEIL"
     gene            complement(80120..80914)
                     /locus_tag="PK218_00400"
     CDS             complement(80120..80914)
                     /locus_tag="PK218_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPW97001.1"
                     /translation="MKIITTPWKNNFLELVSNSKHSIKITSPFVKENVCNEMLIAKSS
                     NVKLELITSFKLGNIHSGSLDIKAIENIINKDGIVKNHSKLHSKIYLFDDKEVIISSG
                     NLTNGGLINNFEYGVYTNDKAIVSQVVTDFDLISKNEKTGTIEPRHIAEVNKILSNIA
                     KSETTKFPKFEIENQEDKNDVIQLSRNSITQSLNGWKLDVFNCIDSIEKEIFTLADVY
                     NFEEVLKQKHVTNTRIAPKIRQQLQNLRDIGLLEFLGNGVYRKLWK"
     gene            complement(81186..81557)
                     /locus_tag="PK218_00405"
     CDS             complement(81186..81557)
                     /locus_tag="PK218_00405"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPW97002.1"
                     /translation="MEIAIIAHDGKKAEMVQFINKNKYLLEKENIKLIATGTTGSKVE
                     AVGIKVKKMLSGPQGGDAQIAARVAEGKCKMVLFFKDPNASHPHEPDISMLIRICDVH
                     NIPLATNEATAQLLLLGLDDL"
     gene            complement(81613..82464)
                     /locus_tag="PK218_00410"
     CDS             complement(81613..82464)
                     /locus_tag="PK218_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HPW97003.1"
                     /translation="MKLLVDSGSTKADWIAIDDNGKVLFTTQTLGLNPEVLEKEEVIE
                     RLEDKFDIAQNKNKATSLCFYGAGCGTDRMKIFLTSIFEEYFPNAKVAVYEDTYAAVF
                     ATTPKDEKAIVCILGTGSNCSYFDGSMLFQKVQSLGYIAMDDGSGNRFGRHLLRGYYF
                     NKMPKELALEFEEEYNVEADYIKANLYKEPNPNAYLATFAKFIIKHKENEFCKKIIKK
                     ELKSFAKNYIMQFENCQEVPVHFVGSIAFYLKDELEIVLNKYNIKMGNVLRRPIDGLI
                     AYHALNK"
     gene            complement(82562..83569)
                     /gene="gap"
                     /locus_tag="PK218_00415"
     CDS             complement(82562..83569)
                     /gene="gap"
                     /locus_tag="PK218_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPW97004.1"
                     /translation="MSKVKLGINGFGRIGRIVFRESMNRDNVEVVAINDLLDVDHLAY
                     LLKYDSVHGRFNGKVEVKDGQLYVNDKFIRVTAQKDPKLIQWDDKEVDVDIVAECTGF
                     FTTVETASAHIEGGAKKVIISAPSADAPMFVMGVNHESVKATDLVVSNASCTTNCLAP
                     LAKVIHDNFGIVEALMTTVHATTSTQMTADGPSRKDWRGGRAASVNIIPSSTGAAKAV
                     GKVIPELNGKLTGMAFRVPTVDVSVVDLTVKVAKETTYEEIMAVIKKTSETSMKGILG
                     YTEDQVVSQDFISDKRTSIVDAKAGIGLNSTFFKIVSWYDNEYGYSSKLIDLAVHISK
                     I"
     assembly_gap    83758..83767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(83768..84620)
                     /gene="pfkA"
                     /locus_tag="PK218_00420"
                     /pseudo
     CDS             complement(83768..84620)
                     /gene="pfkA"
                     /locus_tag="PK218_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139016.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
     gene            complement(84757..89271)
                     /locus_tag="PK218_00425"
     CDS             complement(84757..89271)
                     /locus_tag="PK218_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPW97005.1"
                     /translation="MEENKKNSQFKKIRKVIIRTIIALLVFLLLSAIALTLPFVQTKI
                     AKYVTDDINKEYGVNISIDKVAITFFGGVKLKTVLILDHHQDTLIFLNRLNTSLLDFK
                     NIANSKLRFGDLRFDNLYLNIVNYKNEKDTNLDLFVEALDDGKPGSGNFLMTSKNVYV
                     KKSRFVMMDYNREVPKDVDFTKLNAHLENFQIKGPDVTAKIAEMSFLDHRGLFVKDLI
                     ADFAYTKRNIKLENLKLETENSLVEGKVILNYNKDNKDFSNFNNKVIFDIETKNSKIA
                     TNDIRYFYSEMGKNKVFMLTSKVKGTLNDFYATNLYLKENNNSIIKGDVNFKNLFPRS
                     PGEFFMKGDFKKITSNYKDLTELLPNVLGKKLPTSLEKLGQFNFTGTAEVTQKYIDAD
                     FVMNTALGIIESTLTINNLDNIDNANYKGNVIFDQFEIGDLIGQKDIGKVSLNIDVDG
                     KGFTQKLLNTTFVGDVYKLNYRGYNYQKIVVDGSFKQPVFKGKFFVNDPNLYMDFNGL
                     IDLGKNEKIINFNAKIDYANLDKLNIIKDSIAVFKGDIAINSKGNDLDNFKGDVVLTN
                     AMYQNRKDIYFIDNLSINSSFDEENERKITVNSTDALSGTIVGKYKFDQLKSMIENSI
                     GTLYANYKPNKVKKGQYIKFDFDIYSKIIEIFYPDISISKNTKIKGSISSESDNFKLD
                     LKSPKITYKENTFDNLLLQIDNKNPLFKSYVQLDSINTKYYKVRDFSMINNIQNDTLY
                     FRTEFKGGDKGNDFYNLNLYHTINAENQNVVGLQKSEVQFKDYLWFVNEEDDNQNKIV
                     FDKKLQNFNFDNIKISHENQSVNLNGVLKGTTNKDLKLTFKNVNLNKITPDVEKFKFD
                     GKVNGDIYFKQNLQVYQPTSSLEIDDLMLNDIALGKLNLDIKGDESLKKFYVRSNLIN
                     KNVESFEADGTIEVYDGKTSLDVDLRFDKFNLGVLGKIGGDVITNIRGFASGNARIDG
                     DINQLDYNGRLFVNDTGLTIPYLGVDYSFANNSIVDVTENKFIIRQTEIKDSKFDTEG
                     QFNGFIKHKQFGDWELDLNINSKKLLALNTVDSEDAAYYGTAFMNGSASISGPTEGLA
                     IKVNAESVNGTSIKIPINDSELAEENGYIRFKKPISNNSISKEEKKFNGLELDFNFDI
                     LENADIEVILNRDSGHGMKGNGRGTLLFEINTLGKFNMYGDFQVYKGYYNFKYGGLFG
                     KTFEVKKYSSIVWSGDPMAATLNIDAVYKTTANPAMLIANPSFNKKVDVEVVIGIKGS
                     LMNPEPEFSIEFPKVASTLRSELQYKLDDKDTRQTQALSLLSTNSFLSPEGVTQSQSS
                     NLLYEKASSVFSDIFSTDGGKVSIAPEYVGGDTRPGYETDARFGLSLSSKINERITVN
                     GKVGVPVGGVNESAVVGNVELQYRVNDDGTLNLRVFNRENDVTYIGQGVGYTQGLGLS
                     YEVDFDTFRELITKIFNNKKLLQIIPSKSEVGDDSVLPDHYQFTNDPKHPKKEDKPHP
                     NNQGLPPEDY"
     gene            89373..90395
                     /gene="tsaD"
                     /locus_tag="PK218_00430"
     CDS             89373..90395
                     /gene="tsaD"
                     /locus_tag="PK218_00430"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPW97006.1"
                     /translation="MIQDEVFILAIESSCDDTAAAVLKNNKVLSNIVARQSIHEEFGG
                     VVPELASRAHQQNIVPVIDVALKKANVTKEQLSAIAFTQGPGLMGSLLVGTSFAKSLA
                     LALNIPLIAVNHMHAHILAHFIDEDGFDKPEFPFLALTISGGHTQIVKVKNFFDMEII
                     GETTDDAVGEAFDKTAKILGLPYPGGPLIDQFAKEGNSKKYLFTIPKVKGLNFSFSGL
                     KTQILYFIQKNVAENPNFVEDNKHDICASVQQIIIDILMEKLKLAVEETGITQIAIGG
                     GVSANSGIRNTLKETEQKYGWKTFIPKFEYTTDNAAMIGIVGYQKFLHDKFENAAVVS
                     KARIEF"
     gene            90398..91105
                     /locus_tag="PK218_00435"
     CDS             90398..91105
                     /locus_tag="PK218_00435"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPW97007.1"
                     /translation="MQLFYTPNLTETQTFFVFDKEESKHIVKVLRKKTGDILHVTNGL
                     GYLFTTEITIASDSKCTVKINSFQKQEAPKFKLHLAVAPTKMNERYEWFLEKATEIGI
                     QEITPIICEHSERKVIKTDRFQKILESAMKQSLHYYLPKLNEPITFKDFIKMESQGQK
                     FIAHCEETTRKSLKNELKTNENVLILIGPEGDFSVKEIQQAIATKFIPVSLGNTRLRT
                     ETAAIVACHSVIFKNET"
     gene            91109..92311
                     /locus_tag="PK218_00440"
     CDS             91109..92311
                     /locus_tag="PK218_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPW97008.1"
                     /translation="MNSIKTLFSIVVLIVICQISFAQKQTLFIDKDFNAVMQQAKLEK
                     KPVILMFYASWCAHCNKMKNEVFINQDVINFYTQNYICIASDIESTEGKLLREKLKNQ
                     LIVKSFPTFCYFDDNQNLTNCITGEFKSEEFIKEGVDNLNEANHLQNVKSSFEKEVSN
                     YDKCFSYILMTKRIGFNPTEIAQKYLKTVAANNYYTEENWRLIANGILDFDAPEFIDL
                     VKNKAKFEKVVSKKRVEKKINFVINDNFADYITQNDTVTFNKNRKIAANFKLREIDSL
                     LFHQDLSLYERTYKWNEYNSVLEQNMANFGLKDAQFINNVCANYFIYISDKKMLENAV
                     SWEKTALELNPSLDKYVLISNLLLKNKDYKQGIQYATKGKEFGKSLGFNTTEIQNVLD
                     ELIAKSKK"
     gene            92308..92958
                     /locus_tag="PK218_00445"
     CDS             92308..92958
                     /locus_tag="PK218_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HPW97009.1"
                     /translation="MKSNIKLYLFFFSLVTFNAFSQEIALLKYNGGGDWYANPTSLPN
                     LIKYANQTINTKIKNKPATVEPGSLEIFSYPFVHMTGHGNVVFSDAEILNLRNYLISG
                     GFLHIDDNYGMDEYIRREIKRIFPNNPLVEIPANHAIFQKPNVFANGLPKIHEHDGKR
                     PQAFGIFENNRLILMYTFETDLGDGWEDPEVHNDTKEAREKALKMGTNIVNYIFNN"
     gene            92961..94052
                     /locus_tag="PK218_00450"
     CDS             92961..94052
                     /locus_tag="PK218_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPW97010.1"
                     /translation="MYSSKTIAYGIVKAVGILLLFALLLLFFYKIQTVILYLVISLIF
                     SLIANPIVEFLKRKLKFKNTLAVITTLVLFLLLIIGVISMFVPLIISQSSNLSLLNTH
                     QIEKNVLDIATQFSQFLKKHNIESENIINPNQWLSKMNFAVFTNFFNSLLTTISNIGM
                     GLASVFFITFFFLKDKLLFLSSAKAILPDAHEDQILNSVTKINLLLSRYFIGLIIQLA
                     LIFILYLIVLVLFGVQNALIIALICGILNIIPYIGPIIASVLAAVLTMMGTINSDFQT
                     VTLPTTIYVLIGFFVVQLIDNNINQPIIFSNSVKSHPLEIFLTILIGGFIFGIVGMII
                     AVPFYTILKVIGKEFLPENKIIKLFTKNI"
     gene            94055..95236
                     /locus_tag="PK218_00455"
     CDS             94055..95236
                     /locus_tag="PK218_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW97011.1"
                     /translation="MNKTILSEEIQAFINEHVSANVSDLALKKSKFLSFDWTLILNQI
                     EAKQKAKIKLPTWFSTKNIYYPSKLSIEQTSSEKTAAYKSSLVSGESLIDLTGGFGVD
                     DYYFSKKIKHILHCEINAELSEIVTHNFEVLQQQNIECFAEDSLTILDKLKRNFDWIY
                     IDPSRRNDAKGKVFMLKDCQPNVPELLDFYFEKSNNIMIKTAPILDITAGLSELKNVK
                     ELHIVAVDNEVKELLWILEKKYNSNIKIITCNILKEHQELFSFSLNDDSSNFDYSLPK
                     KYLYEANAAIMKSGGFTEIGTQFGIEKLHQHSHLFTSDTLIDFPGRVFEIETVMNYEK
                     SSMKTHLSNKKANITTRNFPETVDNIRKKWKIQDGGNLYCFFTTDINNNKIVLLCKKL
                     K"
     gene            95239..95805
                     /locus_tag="PK218_00460"
     CDS             95239..95805
                     /locus_tag="PK218_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97012.1"
                     /translation="MKKFISIVLLLLNFFAFAQKPCEIDTDITDSIGTYKSTKQFLIF
                     ERNFAGNSTNIYFSLASNNGILNLEFQSIQRSSDFIKANCFDKSSKIYLQLNNGKIVT
                     LLNVGADTCGTLLRSDDGKNNRITSSSFVFTKENFEDLKTSKITFMRIQFSGEIIDYP
                     FKTELVSEMDNKIYQPESYFIDYLKCFD"
     gene            complement(95815..97119)
                     /locus_tag="PK218_00465"
     CDS             complement(95815..97119)
                     /locus_tag="PK218_00465"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW97013.1"
                     /translation="MNIPHADKPRIVIVGGGFAGIAIAEKLKNKEVQVVLIDKHNYHT
                     FQPLLYQVATGGLEAGSIAYPLRKVIQEYDDFYFRLTSVKEIDTKNQKIISEIGDLHY
                     DYLVIATGSKTNFFGNKEIERNAMSMKTIPQSLNIRSLILENFEQAVLTNDQSEKDAL
                     INFVLVGAGPTGVELAGALAEMKKAILQKDYPDLDISKMGINLIQSDDKVLSTMSEKS
                     SIAAEKFLNKLGVKIWKNVRVTNYDGRTVSTNSDLSFETATVIWTAGVEGAPISGLNS
                     DAFVERVKRIKVNEFNQVNGYENIFALGDIASMETKDYPKGHPMMAQPAIQQGKLLGE
                     NIIKLIKNKPMKPFQYNDKGSMATIGRNLAVVDLPKYHFSGVFAWFVWMIVHLFSLIG
                     FKNKAVVFLNWVYNYIRFDREGRLIIRPYKKRSFVTFTSDEV"
     gene            complement(97123..97440)
                     /locus_tag="PK218_00470"
     CDS             complement(97123..97440)
                     /locus_tag="PK218_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPW97014.1"
                     /translation="MDLIQEDWKSLLKNDTNAVILDVRTKEECDEGIIPNAIMIDIYK
                     GHDFIEELEELDKTKNYYVYCKAGGRSAQACGIMKQLGFETAYNLIGGFSNWNGDVTF
                     PKV"
     gene            97597..98058
                     /locus_tag="PK218_00475"
     CDS             97597..98058
                     /locus_tag="PK218_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPW97015.1"
                     /translation="MEIEKIIKSTINISAAKKVILNMVYTNTLVGEKFLEKLKPYDLS
                     SEQYNVLRILRGQKGKPANMCVIQERMIAKNSNTTRLIDKLLLKQLVTRDVCPDNRRK
                     IEVLITQKGLDLLTELDPIVIQHEEDFASNLTSTELEELNRLLEKYRNTIN"
     gene            98062..98694
                     /locus_tag="PK218_00480"
     CDS             98062..98694
                     /locus_tag="PK218_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPW97016.1"
                     /translation="MSNFIQDANWRYATKKFDASKKVSKEDLDTLKEAIRLSSSSYGL
                     QPYKVFIIENPELRRKLQPAAWNQSQIVDASHVVVFANVVNFGDEEIDNYIQNLIKTR
                     GIELASVQGYADFMKMKINPLSIEEKKNWTSKQTYLALGNLLNAAAELKIDVTPMEGF
                     EEDKVNEILGLTQLGLNASLIATIGYRHEEDATQHFAKVRKSNQELFINL"
     gene            98720..99289
                     /locus_tag="PK218_00485"
     CDS             98720..99289
                     /locus_tag="PK218_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPW97017.1"
                     /translation="MKNVKTIALALVIALGTTVASAQNKKINIEKSKINWVGKKVTGQ
                     HSGTISFESGTLVFEKKALKGGSFVVDMSSINVTDLEAGKGKEKLEGHLKADDFFGTD
                     KFATSTLDFKKIASKGNDVYTVTANLTIKGITNPVTFDIAVKGNTATTKVIVDRTKFE
                     IKYGSGSFFDNLGDKTISDEFELNVSLVF"
     gene            99420..99989
                     /locus_tag="PK218_00490"
     CDS             99420..99989
                     /locus_tag="PK218_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPW97018.1"
                     /translation="MKKIAVIDNYDSFTYNLVHYLEDLNAIVTVFRNDELELEELEFF
                     DKIVLSPGPGIPDEAGLLKAIIQKYYKSKSILGICLGQQAIGEVFGGTLINLKKVHHG
                     VASKVKITVPNEELFKNLPDEFEVGRYHSWVISRVNFPENLIITSEDKNGEIMSISHT
                     IYDVKGVQFHPESILTPLGKKILENWIMS"
     gene            100399..101022
                     /locus_tag="PK218_00495"
     CDS             100399..101022
                     /locus_tag="PK218_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPW97019.1"
                     /translation="MTEFNEKQIEILQAAENLFSEVGFDGTTVRAISKKANINVAMIS
                     YYFGSKEKLLESLVIYRISGMQMDLENLFLEHISPIEKIEKLVAFYVQRIYSNRCMYQ
                     ILHFEMSNKKRDLDLKAFTDVKNSNLQLLANIIHEGQALNLFKKNVTIELIPPTIIGS
                     FTHFYMNKSYYQKLLGLTTDVLLENYITTTFTEHLQKTIKALLVYEN"
     gene            101012..102337
                     /locus_tag="PK218_00500"
     CDS             101012..102337
                     /locus_tag="PK218_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW97020.1"
                     /translation="MKINKMIFSIILALVFNNIEAQEKKLLTLKEAIQIAVTNSDQAT
                     LAKTKVITSKLELDNTKNNSYPSLKASGQYLRLSNANIDSNIQPNSSASGEPSKPLKA
                     EQLMLGQVNASLPIFSGFKLKNSIKTSESLLESEKFTEKHSKEQIGLQVVELFSSLYK
                     TQEMTLLIEDNLKTANQRVKDFTAMEENGLIARNDLLKAQLQVSNIQLALDNSKKNTA
                     ISNYKLITLLKLPENTTIEIDIEAIKKELKENQKAFSGERNDLKALEYKKAASESAVK
                     VAKGNYYPSIALSAGYIALDINNIVTVTNAMNFGVGFSYDISSIFKNNKQVKLAQNKT
                     KETEIAIHLLQNQINEDLFEAKENYALSLKQSLVYEKAVEQATENYRIVKDKYDNSLS
                     DTNDLLEADFEALQAKINQALSKADVAQKYYELQFANGTLTSSLNITQN"
     gene            102351..103430
                     /locus_tag="PK218_00505"
     CDS             102351..103430
                     /locus_tag="PK218_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HPW97021.1"
                     /translation="METKKTNTKFKLIFIVLLVIGISYGGYKFIKSLSHETTDDAQVE
                     KKMNPIIPRVPGYITKVYVKDNQQVKQGDTLFVIDNSDYLVKVEEAKAAVAIAENSME
                     VSKADIGTSQAGISVSNANADSALGSIETAKIRLRRASNDFDRYSNLYKNHSITKQQF
                     EQAEAAKQEAESQLQIIQNQQKASSYQRTVAAARNNVSTKQVQVAQANIQKTKAMLDA
                     ANLNLSYTVVTASIDGQVSTISIQPGQLVQPGQSLFYIINNQETWVVANFKETQLDKM
                     RIGQKVKLQVDAFPDVTFEGELASFSPATGSKFSLLPPDNASGNFVKTIQRLPVRINF
                     TNQNDKEKLQLLRSGMNVAVDVVLK"
     gene            103474..105063
                     /locus_tag="PK218_00510"
     CDS             103474..105063
                     /locus_tag="PK218_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HPW97022.1"
                     /translation="MATAAVVQEDLVEYGFRRVIITITAVMCALLEIVDTTIVNVALN
                     NMRGSLGATLTDIAWVITAYAIANVIVIPMTSWLSQQFGRRNYFATSIITFTVASFMC
                     GQATNIWELVAFRFIQGLGGGALLVTAQTIITESYPVAKRGMAQAIYGMGVIVGPTLG
                     PPLGGYLVDNYSWPYIFYINIPIGIIATFLTLTFVRSPKYGNKLKANQVDWLGIFLLS
                     AFVGSLQYVLEHGQQDDWFNDAAIVALSATSVFGLFFFIWRQMTYEFPIVNLRVLKDG
                     NLRIGTVMTFILGFGLYGSTFIIPLYTQSILGWTATDAGLILVPSSLTTAFLMPFIGR
                     MIQKGIPQSYMVAAGFMIFFIFSYWMHVEMTPDTGVEHFFWPLILRGVGLGLLFVPIT
                     TLSLSTLSGKSIGEGAAMTGMMRQLGGSFGIAIITTFIARFNQEHRVNLISHLDPTRV
                     NVQDRIAALQRGFMSKGFSYNEALDKAYKVLDFTVSKQSTVLSYMDVFLYLGILFLAC
                     IPITLLIKRSKNKVDLSEAMH"
     gene            105169..105516
                     /locus_tag="PK218_00515"
     CDS             105169..105516
                     /locus_tag="PK218_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97023.1"
                     /translation="MGIFNNHDKKVIAQLCKKSEDISNEITKEINEFIDDLNSEYEET
                     KVAVTEFSSFISELETKLSPEEINKLHSFSTRLNKVKRCAKKGVEAMRELARDQRKAT
                     RETIREYEQYLYI"
     gene            complement(105518..106276)
                     /gene="yaaA"
                     /locus_tag="PK218_00520"
     CDS             complement(105518..106276)
                     /gene="yaaA"
                     /locus_tag="PK218_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HPW97024.1"
                     /translation="MKIVISPAKSLNFEKELPTTQHTEPAFLKQAVTIQKTLKKKKPK
                     QLSALMSISDKLAELNWQRNQDWSVPFTTQNARPAVYAFDGDVYTGLDVYTLSEAKLE
                     ALQSKLRILSGLYGLLKPLDLMQPYRLEMGTTITIGTHKNLYAFWKKTITKALNEELE
                     KDELFVNLASNEYFSAVDVKTLKVPVVTPEFKDYKDGKLKMISFFAKKARGMMVRYII
                     DTNAKTIEDLKGFNYEGYSFDANLSKGNTLVFTR"
BASE COUNT        35626 a        17704 c        17485 g        35976 t
ORIGIN      
        1 cattagtcgt tcggatatta tcccaataac cagaaggatt ttgagcaaaa ctcatcatcc
       61 caactaaaag taaaccgaat agtgttcgca taaaaatttt attttaatct atctaaacaa
      121 taaaagtgcc aaaaggcact ttaaagttaa ttttattgat tttattagag agtaatactt
      181 tcgccaatat ctaatagcat taaatcctta tcggcatcga agaatttttt cttagcatcg
      241 tctttattta tctcaataaa accaaaagta tcataatgac agcctaaaat cttatcgcat
      301 gctacaaaat cggcggcaat gatggcatct tcaatatcca tagtgaaaca attgcctatt
      361 ggtaaaattg ctaaatctag ttttgtgcgc attggaatta gcttcatatc cattgttaaa
      421 gcggtgtcgc ctgaaatgta aatgtttttg tgttcacctt cgatgacaaa accacctggg
      481 tttcctccat aagttccatc tggaaaagca cttgaatgca ccgcgttgac atattttact
      541 tttccaaagt caaaattcca acttcctcca tgattcattg gatgaaattg aaaccctttt
      601 tttccaaagt aatttgcaat ctcccaattg gaaacaataa ctgcgtttgt gtttttggcg
      661 atgacttcta cgtctaaaat atggtcaaag tgcgcatgtg taataagaat atagtcagct
      721 tttatagcgt ttatatctat atgagccgct ttttcatttc cggttataaa aggatcgatg
      781 ataatgtttt ttccaccaac ctcaacacca atacaagcat gtccataaaa agtaattttc
      841 atatttatgt gttttaaatt aaacgtaatt tactactata tctgatataa agaatattag
      901 cgacaaggct aatagtatag acaaaaggaa tgtgcttatt gctagttttt ttaattcgga
      961 atctaaaagt tttgggtttt tgtttctagc aacagtcatc aaatgactaa tcattggaaa
     1021 ataggctact acaaatatgt attggtcaat atggaaatca tttaaaaaag caaatactaa
     1081 gaccataacc atagcagaaa ttactaagaa gaaatggtat ttcttagccc atgctccacc
     1141 catttttaca acaatagtgt tctttcctgc ttttttatcg ctttcttcat cgcgcatgtt
     1201 gtttaggttt agcactccta cactaagaaa accaatagca gtagcaggca gtaatagcaa
     1261 ccaatccata cttttggaaa acataaaata cacaccaaaa gtgcttacta atccaaagaa
     1321 cgtaaaaaca aataagtcgc cataacccct atacccatag gctgttgagc caaccgtata
     1381 acgaatggcc gatgctattg ccacaattcc taatgctaaa aagaataagg aataccacaa
     1441 atagctttct ttgaatgcaa agtaaattaa gagtaacgca aatattaatg tgattagaga
     1501 ggtgattaac attgcttttt tcatttcttg gggagatata acgccacttt gaatggcgcg
     1561 cattggtcct actcgatctt cattatctgt tcctttgact ccatctccat aatcgtttgc
     1621 aaaattggaa agtatttgca atccaagcgt tgttaataat gcaaaggcaa caatattcca
     1681 attgaacatg ccttgtgaca ttgcgtaaaa acttcccaca attattcctg ataccgataa
     1741 tggtaacgtt cgtactcttg cggcttcaat ccagtgtttc attttttatt gtttattcgg
     1801 ttatgcggtt atttggttat ttattatagt tctcgacatc ttttttatta cgtttcactt
     1861 tataaaaaaa cgttcgaaca gacgagaact taaaactctg aattctaact tctgaactca
     1921 taattctcta catccaatta ctgttctgtg tttctagttt gtataaccac aggtttacta
     1981 attgatttat tctatcaaat tttcctaaat agaacgttac gtttccgcct gcggtgtgca
     2041 aagttagtga acctatgttt atgtttttgt gccaaaacaa ctgtgatgtt gttagcgctt
     2101 gtattttttc gatttgaatg atttctttat ctacatccca agcaccactt tgttttataa
     2161 tgtaatcgtc gtttacaaac aaacggtaat ttctaaatcc gaaacacaat aaaatcgtta
     2221 tggcaacggc gtagaaaact gcaatggata cataatcgaa aataactggt tttacccaat
     2281 gaccgacact gtaaaacacg gataaaggta ctataattaa tagaaatatc gaaaagccta
     2341 gttttctaaa gttgggtttt agcatttcgc cttcttgtgg aagttcttta aagagtaact
     2401 ttatgatttc atcggcttct tgggaattac agcctgggat ttcgatgatg tttttgttct
     2461 tattatcggc atgaccaacg gcttgcttta tttttatttc taaaacattg agtttctttt
     2521 gaaaatagtt gcttgagact ttggttattt gcactttctc tggtttaagg atggtgcttt
     2581 tggtgttgat taatccatag gacaagacta gtgaaccttt ttgcttggct acggtatatc
     2641 cgaaaaactt aatgactgtt cgagcgatgt ttagtataaa cactactgca aacatgacca
     2701 cgataaacat cagaatgacg taaataatgg gtttgttttc tattaaagag tttacattat
     2761 cagaactaac gacatcggtt tttccggctt ggcttaatcc gtcgtaaatg gtgaaaaaga
     2821 atgttagtag caacccgatg gtgcgaacat aattggaggt tatccctatt ttgaacaaac
     2881 ttactaaatc tattttcaga aatggaaccg agaaatttgt ttttgggtcg gttatttcgg
     2941 taaactccgt tgtttttact ttgttttcgt tttcgaggag ttttgatttt aatgctaacg
     3001 ccatttcatg tgaaacagct ttgatgttgc cttcttttgt atcgctacct gcagtatcaa
     3061 catctaatgc atagacatta atgattcggt ggacgatgtt ttgcgaaagg tttacttgct
     3121 gtattttgtt gagttgaatg cttgttcttg ttttattgaa aataccttcg ctgatgatga
     3181 actcttcttt ttggtcgtct atataaaatg taaagtttat gtatctcaaa taagcaatta
     3241 caccaactat gactactata gctagtaatc caagatacaa atagagtaca ttgattgtat
     3301 ctccttttag cacaaaaatg aatgctattg gtaggaaggc acgagcatac ttttgcaaac
     3361 tgtctgcaaa gaggacgatg attccaattt tggattgacg ttgtgggtga ttgaaatggt
     3421 tttccattta caattgaatt tgaattttac ccatcaataa ttgtttgatg tcttcggctt
     3481 gtggtttaag tattcctgga acacctaagt cactggcaat gccacctgct gtaaagagtt
     3541 ctactttggc taacccaaag ttacgctcta gtaaaccttc atgaagtgca acgtgttgta
     3601 ctctattata aggaattacc atagttgtag tagcgattat tccatggcga aaaatgacgt
     3661 cgtgttctct aaaggcatag gctttctttt tgaacgatag tcttgtaaaa aacaatatga
     3721 ttactaatag aaaagcataa ccactccaaa taacggtttg atttcctgct gtttcatcaa
     3781 tgaagttgat ggctaaaata gcgagtactg ctataaatag aaacgctatt gcaatcttta
     3841 ttagcattac tttccaataa cttggatgca atggcgttaa tgaaatggct tcgtgctttg
     3901 gaagttgatt taggtcaagt atttcgtttg tgaacgtttc catttaggta gttgtttgtt
     3961 ggttctcgat ataattttta ctccgttaca ctccgtaaaa tcacttgaac agacgttatt
     4021 atggcaacca tttcttttcg aagtttggtt ttcttttttc aaggaaagcg tctctgcctt
     4081 ctttggcttc ttcggtcatg taggcaagtc ttgttgcttc accagcgaat acttgttgtc
     4141 ctaccatgcc atcgtcggtt agattcatgg cgaactttag catcttaatg gatgttggtg
     4201 attttgccaa tacttcttgt gcccattcgt aggcggtttg ttctaactca tcgtgtggta
     4261 ctacagcgtt taccattccc atttcgaatg cttcttgggc ggaatagttt cttcctaaaa
     4321 agaagatttc tcgagcgcgc ttttgtccaa ccattttggc tagataggct gagccgtatc
     4381 caccatcgaa actggttaca tcggcatcgg tttgtttgaa gatggcgtgt tctttactgg
     4441 ctagtgtcat gtcacagact acatgtaaac tgtgtccgcc gccaactgcc catcctggta
     4501 ctacgcagat tacggctttt ggcatgaaac gtatcattcg ttgtacttct aatatattta
     4561 gtctgtggta cccgtcgtcg cccacgtatc cttgatgtcc tcgtgctttt tggtcgccac
     4621 cagagcagaa agaccaaatg ccgtctttgg aacttggtcc ttcggcagaa agcaatacta
     4681 caccaattga ggtgtcttct tgtgcatcgt agaatgcttg gattagttcg gctgtggttt
     4741 ttggtctaaa ggcgttgcgt acgttgggtc tgttgaacgc tattcgtgct actccattta
     4801 attttttata ggtgatgtct tcaaattctc ttacggtttt ccactctagg gtattcatgt
     4861 atgttggatt taaaaatttt atttttttgt taaaaataac ggcttaatgt ttaaaactac
     4921 tattttagcg taaaattaaa tcatttttta aagataaact caaaacaaga tacattctta
     4981 tgaagaaaat tttagtctca ttccttctgt tatttgttgg aataccatct tttgcgcaac
     5041 cttatcaatt tcaaactatt aaagacattg atgccttgcc tgttgtatca caagataaga
     5101 caggaacgtg ttggagtttt tcctctactt cgtttttaga agctgaaatt cttagaatta
     5161 ctggaaagaa aatggatttg tctgaaatgt ataatgtgaa atacacttat ttggacaaag
     5221 ccgaaaatta tgtaatgcga caaggcaaag cgcagtttgg cgaaggtgga ttaaatcacg
     5281 atgttattaa cagcgctaag aaatatggca ttgtttatga ggactgttat acaggaaaag
     5341 tgaatcctga tgagaaatat gaccatagca agatggaagc agaattggaa gctatagtaa
     5401 agaaagctgt tgccgatact cctgctaatt atccggcttg gaagaaagat tatatggcgg
     5461 tgttggacaa ttacatgggg aaatatgatg cagaagacca aactgtttct gctactgcta
     5521 ctattgacaa agccaatgag aaaacttcaa aatcgttatc gcctaagcaa actttagaac
     5581 tttctaaatt gaagttggat gactacataa cggttacttc ttttacgcac gagccagcct
     5641 atagcaagtt tatcttgaat atacctgata atttttcgaa tggaagttat tacaaccttc
     5701 cgttggatga atttattggg acaattgact atgctttaga caatggtttt acgttggcat
     5761 tggatgctga tgtttctgaa actacttttt ctgctaagta tggtattgcg gtgttgcctt
     5821 ttaaccctaa agatgctgtg gaaattctaa cttctattaa accagagaaa aaggtttcgg
     5881 ctgagtctag acaacagcag tttgaaaact atagtactac ggatgatcat ttgatgcaca
     5941 ttgtaggtaa agtgaaagac caaaatggga atatctatta taaagtgaaa aactcttggg
     6001 gaacgaaatc ggggaaagat ggttatattt atatgagtgt tgcttatatg agcatgaaag
     6061 ccatttcggt tatgctagac aaagatggtt tgagcaaagc gactaagaaa gcactgggat
     6121 tgtcttaatt ggatttagaa agaagcagtt tgtggaatag tgtcttgatt tgttttaaaa
     6181 cttatttatt atccattttt ccatcacata atcttccatc aaatagccat ttcctatttc
     6241 aatatcaact tctccaacaa tactaaaacc aagtttctca taaaagtttt ttgctacatt
     6301 aaatcgattt acattcaaat aaactgcttt ttcaaggtta tttaaagctt ccttttcaat
     6361 aaaattaatt atttttttac caataccttt tccttgcaaa ttgggcaaaa tatatagttt
     6421 atgaactttt gttttatttg aattttcaca atcaaattca taagaagcaa aacccagagg
     6481 tctttcatct tcataagcga taacaaaatg gtgctttttt tcttgtgctt gatgttgtaa
     6541 agaagtcaca ctatacatca tatcaaacat atattctaat tgctctttcg tcaaaatagc
     6601 actataggta actggccaaa caattttagc tatggttcta attgtttcaa aatctttatc
     6661 taaattagga ataattgtta tcatttatct ttttataaca ctttcataca ttgaaatcca
     6721 atcgtcaact gtgattttct gaactaattt ggcaattagt ttaaacggaa tatcatccat
     6781 ttttttaaat cgaatacaac tttttcccat atctaatttt gttttgcaat gcttaggata
     6841 ttcggcaaca aaccaatcca acaacgattt atctgcataa attcccatat gatacaatgc
     6901 cacaaagttt ttttgagaag caatactcat aaaaggcaac gccattttcg aatcacaatg
     6961 atacccatca gggtaaattg aatgaggcac cgcataacca atcatgccat acgccatttt
     7021 ttctgtgaac ccttttggaa gattttctaa aatagtattt ctcaaagttt gcattgccac
     7081 ttttcgttcc tcgggcaaat ctaataaata gtgttcggga gttgttgcta atgattgcat
     7141 agttgaagtt tttatgtatc aaaaataaaa taattttaat agatttacct actgattatt
     7201 tataagtatc atttatgtca aaaaatactc ttcgagaaga taaaacctgc ttgaattgca
     7261 actatgttgt agaaaaaaaa ttctgtccta attgtggtca agaaaatatt gatactcgaa
     7321 aaaccttcta tcaacttttt gtacattttt ttgaagattt aacccattat gaaaactcat
     7381 tttggaaaac aattcgcaat ttactattaa aacccgcatc gttaacaaag gaatatttat
     7441 caggaaaacg aatgagttat ttagctccta ttcgattgta tatttttata agttttgcaa
     7501 ccttcttttt attaagtgta attcctgata atgaagatac tcctgatgta aaagtaaatt
     7561 caaaaaaagc tttagtagca acaaaaaaga aagaaaaaga tactattttt aaagaacaag
     7621 aaaaaattat aaaactagaa caattaggat tattaactaa aactgagtca gattcattac
     7681 agaaattatt tattcaaact aaactcaaac aaataaagga taagaaaaac aatttcacac
     7741 ttttaggttc tagttataat tctgttgaag aattagattc tttacaaaaa cacggaaaac
     7801 catctgaaaa gcttggtgct tttgaatatt ggattactcg aaaaatgcaa ctagtaaacg
     7861 ataaatatga cagtgatgaa cttaaagaaa aatttgcaga atcgtttatt cacaatctgc
     7921 ccaaatctct tttcatatac atgccattat ttgctttagt tttatggtta tttcagaata
     7981 aaaaaaagtg gtattatttt gaccatggta ttttcacact acattatttt tcctttctat
     8041 tacttgcaat tcttattctt agaatcatta attacttatt tgaatttata aatgattaca
     8101 cttttttcaa catattagaa tatatttttg caactgtcgc aattctatat atgttttatt
     8161 atttctttcc agctcatcac cgcttttatg gtgaaagtag agttgtttct gcatgtaaag
     8221 gaatagcaat gtttattatt aacataattt ttattgtgat aatcctttct gttatggcca
     8281 tttatacttt tattaatatt cattaaaact tatgtttaaa aaaatatcaa cctccatttt
     8341 tatagttttc ttacttgtta gttgttcttc aacaaaaaaa ataagttctg agaaccttac
     8401 acaaaaatac atttcatcta tttctgaaaa tgcattaaaa actaacctaa ctatcattgc
     8461 ttcagacgaa atggaaggaa gagaaacagg aagcgaaggt cagaaaaaag caggtagata
     8521 tattattgaa caatatcaaa aagctggaat atcgtttcca aaaggagcta caagttatta
     8581 tcaaactgtt ccggcagctt atttaaatgc taaacgaaac gaaaatctct ccgaatctga
     8641 aaatatttgg gcattcatcg aaggaacaga taagaaagag gaaattgtgg tagtatcagc
     8701 acactatgac catgttggaa ttaaaaatgg tgaagtattt aacggagcag acgacgatgg
     8761 ttcaggaact gttgcactaa ttgaaattgc aaaagcattc caaaaagcaa aaaacgaagg
     8821 caatggacca agacgttcca tcctaattct tcatgtaact ggcgaagaac acggattgca
     8881 cggttcaaga tattatacgg aaaacccgtt atttccacta aaaaacaccg ttgccgatgt
     8941 aaacattgac atgataggac gccgcgatga tttgcacaaa gacaccaaca actacattta
     9001 cgtcattggc tccgattatt tatcaagtga tttgtatgcc atttgtgaaa gtaccaacca
     9061 gaaatatatc ggtttaaccc ttgattataa atacaacgac aagaaagaca aaaaccgtta
     9121 ctactatcgt tctgaccatt ataattttgc taaaaacggc attccgtctg tgttcttttt
     9181 taacggttcg catgccgatt atcacaaacc tacagacgta gttgacaaaa ttgaatttga
     9241 tgctttaacc aaaagaacca aatatgcatt tgcagtagct tgggaaattg caattagaga
     9301 aaaaagattg gtcgttgata aaacgaatta aatgtaaaaa agtagaagtg tttcaaacaa
     9361 aaaaatcctt tcaatttctt gaaaggattt tgctttttgg gtgaaagacg ggtctcgaac
     9421 ccgcgacctt cggaaccaca atccgacgct ctaaccaact gagctacaat caccatttgt
     9481 taagcgagtg caaatatagt gcaaaagtta tttctcgcaa aaattatttt agatttttta
     9541 tttcaaatcg gataagctat tgacagccaa atacctttct accgtaaatc cttcggcgta
     9601 gtcaacacca accaatcgac cgaaatcttg cgagcgataa tgaatacttt ctaagaagtt
     9661 tttggttgct attggtgtaa cgggttcatt ggaatcttcg gtgtaaaatt gtgaactgta
     9721 agctaaaatt gatttcgttt ttacttcaat gaattcacta atatcgacta caaaatcggg
     9781 ttcgatgttt ttccattgaa tgtaatgata gactactttt ggtcgccatg cttcttggct
     9841 ttcattattt aaactagttt caatttttcg caaacccgag aggaaacaag catccgaaac
     9901 taatttgctt ccttttccat ggtcaatatg tctatcgtca atggcgttgc aaatgacaat
     9961 ttcgggtttg tattttcgaa tcatttgaat tacttttatt tgatgtgttt catcatttat
    10021 aaaaaagcca tcacgcatat ctaaattttc tcttacagta acacccaata tttcagaagc
    10081 tttctttgat tcagcatttc tgatttcaac tgaaccacga gttcctaatt caccacgagt
    10141 taaatcaaca atgccaactt tttttcctaa ggaaacttct tttgcaatcg ttccgctaca
    10201 gcctaattct acatcgtcag gatgtgctcc aaaacaaagg atatctaatt tcatttttta
    10261 attcttttta caaaaataca atttatagaa tacaccgttt catagtcata gctttattga
    10321 cggtttcgat gtattccgct tttggattgc tgtcttttgt gatgccacaa cccacaaaaa
    10381 tttcagctct attctcttta attttcatgc aacgaagatt gacaaacaaa tcggaatact
    10441 gtgttctaaa cgtggcgaag tcaatgttta actcacccaa aaaacccgaa tagaattctc
    10501 tattatatcc ttcgtgttga agtataaaat ctttggcagc ttctcgtggc attccgcata
    10561 ctgccgatgt tggatgtaat gcgttaatta ttttttcaac tgctttcttg tttttcagtt
    10621 ttccagaaat agttgttttg atatgaaaaa tggaacccgc attggcagta aatggttctg
    10681 aaacatttaa ctcttttgta aattctttag tattttcgac aataaaatca gtaaccaact
    10741 gttgttctac ttcttctttt ggtgaccatt ttacaacttt aatgtcattt gctttttgag
    10801 ttcctgctaa tgctactgtt tcaaattcta attcttttac tttgactagt tgttctggtg
    10861 ttgcacccat ccacaaccct atttttgggt gataaaagca atatttaaat gccgatggat
    10921 aattttgaag cattctttta aaacaaactt caaggtcaaa agaattcaat tcaacaattt
    10981 cacgtcttga agtaactact ttttgaaata atccattatc aattgctgct actgatttcg
    11041 ttactaattc ttcaaaaaaa tcatttcctt gttttggaga attctcgttt tgaattgcat
    11101 caaaaaaata ggtttctgaa ttgttttttt ctacaaaaac atcacaaaat tctaacggta
    11161 aaaatggagt tgtactgttg tcaaaagcgg caaacacaaa acctttcttg tcaaagcttt
    11221 ctaaaaaata taaatgatca tttttttgaa aaagcccaat tgttttttct gaatttggct
    11281 tacaaaaaat cacaaaaggt aagttttgat cttgatgtgt ttttatttta agaaataaat
    11341 cagtcattgt gtctttttgg caaaaccatg gttgtcaact tgcatattga aattaatcta
    11401 tcttgttcat cggtaatttt tatttcccaa agatgaatgc ttcttccttt atgaacaatt
    11461 cttgctgtac caaaaacgat accctcgcgc ttgcttctaa catggttagc cgatatttca
    11521 atgccgcgaa cttcttgttt ttcgggattg acatacatta ttgaagctgc gctacctaca
    11581 ctttcggcta aagcaacact agcaccacca tgaagcaatc ccatgggttg gtgtactttt
    11641 gaatttacag gcattttagc ggttaaaaaa tcttcacctg catcgatgaa ttctatttct
    11701 agagattcca tcatggtgtt tttgcacaat tcgttgcaga gatttatgat cttatcttta
    11761 tcaaatgaca tcgctttttt ttgtaaaaat agtatttatt tagcatttga aaataaaaca
    11821 gaagtcaaaa taacattatg attatacaaa aaagacaatt ttaacgttat ctataaatga
    11881 taataaattt gaaatttcaa aatttttcaa attattgttt atttttacac aaatatagag
    11941 atactatgcg taattttatt attctacttt tcattggcat ttccatttca ttgagttctt
    12001 gtcgtcaaga ctttgttttt gaagccagta ctggagattt aactttctca aaagacacca
    12061 tttatttgga tactgttttt accaacattg gttcaagcac ttatacctta aaagtgtata
    12121 accgaagtaa taaggacatt aaaattcctt taattaaatt gggcaaagac gattcaaaat
    12181 accgaattac ggttgacgga atgaccggtg aaaacaacca tacttttcga gatgtagaaa
    12241 tgctagccaa agatagtatg tatatttttg tagaagtaac tgccgatgta gccgattcta
    12301 atccagctga ttttatttat acagaccaaa ttcaatttga tactggaacg aatttccaaa
    12361 aagtagattt ggttactcta atacaagatg cgtattttat ttatccaagt cgaactcaga
    12421 atcctgataa ttcttatacg tatgaagaaa ttaatttagg tgtagatgct gatggaaacc
    12481 caataccttt tcgagcgagc gttttagacg agaatgatcc agttaatggc aacgagttta
    12541 tttggaataa ttccaaacct tatgttatct atggttatgc cgtagttcct gatgggaaaa
    12601 cgcttaacat agaaccaggt gcacaaattc attttcatgc cgattcagga ttgattgttg
    12661 gaacgggtgg aactttaaaa tcaattggta cattacaaaa tcaaattgtt tttgaaggtg
    12721 accgcttaga acctattttc tctgatgtac ccggacaatg gggaaccatt tggttaaccg
    12781 ctggaagcac ggcgaacgaa atagactata ctaccataaa aaatgcgact gttggtttac
    12841 taattgatag caacgatgga aattttatga ccatcaaaaa tacacaaata tacgattcgt
    12901 ccaatcttgg aatttatgct aaaactgcaa aaattaaagg cgaaaacatt gttatcaata
    12961 ctgctggagt ggcttcacta gcatgtactc ttggtggaag ttatgagttt aaacattgta
    13021 cgtttaacaa taattggtcg agttcgaaac aagttgccgt gttgttagat aattattatc
    13081 gtgatgaaaa caatgcacaa gttccttttg atttaataca agctgatttt atcaattgta
    13141 ttatttatgg ttcaaacaca tacgaattaa ggtttgacaa aggggaaact ggaactttca
    13201 attcgtcatt taccaagtgt ttagtaaaat ttggtactag caacgatgca ctttattctt
    13261 ttatatatga cgagaataat attaaacgaa ataaaaatcc gaaatttcta aacattttta
    13321 ctaatcaatt aaatattggc gtagattctg aagcaaaaga atttggtgta gatgctgatg
    13381 ttccgttgga tattttgggt atgcctcgaa ctgctcctta tgatgttggt gcctataatt
    13441 gggtaacttt tcctgaagat aattaatttt ttgtaacttt atcaggttaa ccaataaaac
    13501 ctagtaatat gaagtacaaa actttaacag tagcattagc cttgctgtgc tttgtttctt
    13561 gtaaaaaaga agcagcaaaa accgaaactg aaactactac tgaaccaaca gaggaagtga
    13621 cgatgacaat gcccgactcg gctacaatag ccaaagcttg gcaagattat gcgacaccag
    13681 gagagcaaca tgctctaatg gcaaaagaca acggctcttg gacagaagat atgacctatt
    13741 gggaacatgc aggagccgaa ccttcaaaaa tgaccatgac tgccgaaagt aaaatgatta
    13801 tgggcggaaa atttcaagaa atgactcata aaggggaatt gtatggaatg ccatttgaag
    13861 gaataagcac ttttggctat aacaatgccg aggaaaaata catttccact tggatagata
    13921 atatgtccac tggaattacg gtattaaaag gtacatatga cgcaccaact aaaacttaca
    13981 aaatgattgg tgaagtaata gacccaatca caaagaaacc aaaaacttct agggaaacga
    14041 ttaccattat tgatgataat actcaaaaac tagaaatgtt cgatgtcggg tttgatggta
    14101 aagaatttaa gaacatggaa ataatcatga aaaggaaata ataatttaaa agaccgatat
    14161 tctcggtctt ttttttattt taatttgagt tgcacaatcc atcaaattga aataaatttg
    14221 caactacaac aacacactaa ttacgaaaca aatgattcat ttcttcggaa acgaaagtgc
    14281 tacagttttt gccatacaat cgcaaaacga actttcggca gaaaccatct caaaattaaa
    14341 ttggcttttg ggtaacacca ctaaaataga taaatccgtt ctcgcggatt tttttgttgg
    14401 tccacgtgcc gcaatgatta ctccttggag caccaatgcc gttgagatta ctcaaaacat
    14461 ggggattgaa ggcattatta gaatagaaga atttgaaaaa gtgactcaag attttaccga
    14521 ttttgatcca atgctttctc agaaatattc tgaattacat caagatattt tctttatcaa
    14581 tgttgagcca gaaccgattt tagacattga tgatattgct gcatataacc aacaagaagg
    14641 attggctttg agttcggaag aagttgacta tctcaatact ctttcgacta aattaaatcg
    14701 gaaactaacc gattctgaaa tatttgcttt ttctcaagcc aattcagaac actgtcgtca
    14761 taagattttc aacggaacct tcgttattga tggtgaagaa aaaccaacat ccttatttaa
    14821 attaatcaaa aaaacatctg aaacaaatcc taacgatatt gtttcggcat ataaagataa
    14881 tgtggctttt gtaaaaggac caagagttac acagtttgca cctaaaagtg ccgacaaagc
    14941 cgatttttat gaagaaaaag aattcgactc cgtactttct ttaaaagcag aaacccacaa
    15001 ctttcctact actgttgagc cttttaatgg tgccgcaaca ggttcgggtg gagaaattcg
    15061 tgaccgttta gccggtggac aaggttcatt accattggct ggaacagcgg tttatatgac
    15121 ttcttattct cgtttgagaa aagacagaaa ttgggaaaac ggcatgaaga aaagaccgtg
    15181 gttgtatcaa acgcctatgg atattttaat caaagcttcg aatggagctt ctgattttgg
    15241 aaataaattt gggcaacctt tgattacggg ttctatatta acttttgaac acgaagaaaa
    15301 caaccgtaaa attggttacg acaaagtcat tatgcaagct ggtggcattg gctatggaaa
    15361 actagaccaa gccattaaga agaaacctca cgaaggcgac aaaatagtgg tacttggagg
    15421 cgaaaactat agaattggaa tgggtggtgc tgcggtttcc tcagccgata ctggtgcttt
    15481 tggttcagga attgagctaa acgccattca acgttcgaat cctgaaatgc aaaaacgtgc
    15541 tgccaatgcc attcgtggct tggtagaaag tgatactaac ccaattgttt ctattcatga
    15601 ccatggtgcg ggaggccatt tgaattgtct ttctgaattg gtggaagaca cgggtggatt
    15661 gattgactta gacaaattgc ctgttggcga cccaacgctt tcctatagag aaattattgg
    15721 aaatgaatcg caagaacgaa tgggattgat aattggtgaa aaggacatgg ctatactgaa
    15781 acgtattgct gaaagagaac gttcgcctat gtatgaagtg ggtgaagtaa cgaatgacca
    15841 tcgttttact tttgaatcaa aatcaacagg tgcaaaaccg atggattatg ctttggaaga
    15901 tttctttgga agttcgccaa aaacaattat gactgataaa cacatcgcta ctcattatgc
    15961 tgatgtaagc tatactgctg aaaatattcc aacgtattta gaagaaattc taaaacttga
    16021 agctgtggct tgtaaagatt ggttgaccaa caaagtggac cgttgtgttg gtggaaaagt
    16081 agccaaacaa caatgtgcag gtgctattca gttgccatta aacaacgttg gagtgatggc
    16141 tttggattat aacggcaaag aaggaattgc aacgtcaata ggtcattcgc caatttcggc
    16201 attggttaat cctgctgctg gaagtagaaa tgctattggt gaagcacttt ctaatcttgt
    16261 ttttgcgcct ttgaaagaga atttgaaatc tgtttcgcta tctgccaact ggatgtgggc
    16321 ttgtaaaaac gaaggcgaag atgctagatt atacgaagcg gtacaagctt gttcagactt
    16381 tgctatcgaa ttaggcatca atattccgac tggaaaagat tcgctatcga tgaaacaaaa
    16441 atatcctaac gatgaagtaa ttgcaccagg aacggttatt atttcggctg ctggacattg
    16501 ttctaatatt actaaagttg tagaacctgt tttgcagaaa aacggtggtg ctatttatta
    16561 tatcaacctt tcgcaagatg attttaaact tggtggaagt tcgtttgcgc aaacacataa
    16621 caaaattggt aaggatgtgc caaccattaa agattcggcg ttctttaaaa aagctttcaa
    16681 tacaattcaa gaacttatta aagacagaca aattgttgct ggtcatgata ttggttcggg
    16741 tggattaatc actactttaa tagaaatgtg ttttgctgac acaaatcttg gtgctactat
    16801 ttcttttgag gcgttgccag aaaaagattt ggtgaagatt ttattctctg aaaatattgg
    16861 tgttgtgctt caagcaaaag acgatgctgc atttgaaaaa gcttttgaag gattggaatt
    16921 ttttaaaata ggagcagcta ctaattcaga tagtttagtt gtttcaggtt tcaagtttca
    16981 ggtttcaagt tatagaaaac tttggtttga aacgtctttc ttactcgatc aaaaacaaac
    17041 taagaacaac aaagcgcaag aacgttttga taatttaggt aagcaagcat tagaatatac
    17101 tttcccgagt cattttacgg gagcgatgcc ttctaaaatc acctcgactg cgcatggagt
    17161 gacaagacct aaggcagcaa ttattcgtga aaaaggaagt aattcggagc gcgaaatggc
    17221 gaatgcgatg tacttggcag gttttgatgt taaagacatt cacatgactg atttgatttc
    17281 gggacgtgaa aacttggaag atattcagtt tattggcgct gttggtggtt tctctaattc
    17341 ggatgtattg ggttctgcca aaggttgggc tggagctttt ttatataacg aaaaagccaa
    17401 cacggctttg aaaaacttct tgaaaagaga cgacacgctt tcggttggaa tttgtaacgg
    17461 ctgtcagttg tttatggaat tgggagttat caatccggag catgaagtgc atggcaagat
    17521 gaagcacaac gattctcata aacacgaaag tggatttaca tcggttacga ttgaagagaa
    17581 taattcggtg atgttgtcaa ctttggctgg agctacgctt ggtgtttgga tttcacatgg
    17641 agaaggaaaa ttccatttgc cactgcccga agcacagtac aatattgttt caaaatatgc
    17701 gcacgaaggc tatcctgcca acccaaatgg ttctgattat aacacggcaa tgctttgcga
    17761 caaaacggga agacatttgg taatgatgcc tcacattgag cgttcaacat ttccttggaa
    17821 ttgggcgcac taccctactg atagaaaaga taaagtttcg ccttggttgg aagcttttgt
    17881 taacgcacgt ttgtggattg aaaaacataa ctcttaaaat tgaaatagca tgacattaaa
    17941 aaacgcaatt tctgcattac tattattaat tactatgact acgtttgcgc aaaattcgtt
    18001 gaccgaaatt gagacggata aaggcattcg atacatcacc aattctattg attatcaaat
    18061 aacgggaaaa tattctttta aaggtggcga ccctattgct gtattaaata acgacggaac
    18121 tggaatttat caactgcacg accaacctca acgcgccatg acgtggggca ttgagtgtac
    18181 aaaaaacggt gaattaaaat tcatcaaagg ttttgacaat gtgaaatatg cgcttttcta
    18241 taaattcgac acgagtttgg atggcgaaac ggtagaaaac tggaataaag tagattttac
    18301 cattcatctt aattctttga agatgtttat taatggagaa cgcgaaaaga attttaatcc
    18361 gtagagactt agcggtgcta tgtttattta aaaaccctaa ccaaaaacgg ttagggtttc
    18421 catttttgca acaatgtctt gtcctgaaat agcgatacac aaattttatc gttatggaaa
    18481 accatcaaaa gaatcgctat ataaagcgaa cacaaaaaga ctattctctg tcttttaaac
    18541 ttcatttatt caaaatataa agaaggaata acccatgtac tttgcattca ttcaaaaaag
    18601 cttgattgaa aaattggaat tagataatag caatgattta gacagtttgg tgcaactatc
    18661 tgctaatttt aaattagata ataatattct aaaacagttt gacatcaaac tcataaaaaa
    18721 tattcattaa agcatttcgt aattggagta ctttttttat acttttaaat gcttaaaaca
    18781 aaaaccatgt caaaactacc acatatagga acaagcattt ttaccgtaat gtcaaaaatg
    18841 gcaattgatt ataaagccat taatctggca caaggcttcc cgaattttcc ggtggatgaa
    18901 cggttaactg gtattgtgtc tggaattgct actgaaaacg tacatcaata tgcacccatg
    18961 agtggtcata ctgcattgtt ggacaaaata aaacagctta ctttttcgtc atattctaga
    19021 aatgtaaacc caacaaccga aatacttgta acggctggtg ctaccgaagg gatttttgct
    19081 accattcaag ccttggttta tcgcaacgaa gaagtcatta tccttgaccc aagttatgat
    19141 tgctacgaaa cacctattat attgtgcaat gccaaaccga ttcgaattgc tttaaaagat
    19201 gatttcactc ccgattggga aaccattggc aatgcgataa atcataccac caaactcatc
    19261 attatcaaca atccacacaa cccttcgggt agagtttgga ccgagtatga ttataaccaa
    19321 ttggaaaagt tactcgatag ttatcccaat ttattagtgc tttcggatga agtgtatgag
    19381 cacatcactt ttgaacaaaa acatctctct attcaccacc gtccaaaact ttgggacaga
    19441 agtatttcgg tttcttcgtt tggaaaaaca tttcacatta cgggttggaa aatcggctat
    19501 cttattgcgc ccgaaccttt gatgaacgaa atcaaaaaag tgcatcagtt tttggttttt
    19561 agcgtaaata gcatcgctca aacggcactt gcagaatatc ttaacgtggt tgatgttcct
    19621 gccttgagta gtttttatca acagaaaaga gattatttta gagcacttat aaaagatact
    19681 aaatttacct tgcttccttg tgaaggcact tattttcaaa cggtatcata taataacatt
    19741 agtaccgaaa atgatattga ttttaccaaa agactcgtaa ccgattatgg cgtggcaacg
    19801 ataccaatat ctgcttttaa tttcaacgga aaagacacta attgcattcg tttttgcttt
    19861 gccaaagacg atgctacgct agaaagtgcg gcagaaaaac taatgaaatt ataatacttt
    19921 cttaatgcta tgcgtgcata ctagttattt ttagtacttt tggtaaacta aaacaaaaac
    19981 tatgaatttt tcagcaaaga tgcttcgcga taacgccttg tccgacaaag taattgtagt
    20041 aacaggcggc ggaagtggtt taggaaaagc catgaccaaa tacttcatgg aacttggtgc
    20101 caaagtagcc attacttcca gagatttaga gaaacttaaa aaaaccgcaa cagaactaga
    20161 agccgaaact ggtgggacgt gtttgcctgt gcaatgcgat gtgcgtcatt atgaccaagt
    20221 ggatgccatg cttgctgaag tattaaaagc ttttggaaaa gtagatgttt tattaaacaa
    20281 tgcggcagga aacttcatct ctcctaccga aagattatca gccaatgcct ttgataccat
    20341 aatcgatatt gtattaaagg gttccaaaaa ctgtacgctt gcctttggaa aacattggat
    20401 agaccaaaaa caaaccaact gcaacatatt gaatattgta acgacctatg cttggacagg
    20461 ttcgggctat gtagtgccaa gtgccactgc caaagcagga gttttagcca tgacaagaag
    20521 tttggcggta gaatgggcaa aatatggtat ccgtatgaac gccattgctc caggaccatt
    20581 cccaaccaaa ggcgcatggg aacgattact tcctggcgat ttggctgaga aatttgacat
    20641 ggcaaaaaaa gtaccgctac gaagagtggg cgatcatcaa gaattggcaa acttagcggc
    20701 gtatttagtt tctgatttct ctgcctatat aaatggtgaa gtggtaacta tcgatggtgg
    20761 cgaatggctt caaggcgcag gacaattcaa cattcttgaa aaaataccac aagaaatgtg
    20821 ggacatgcta gaggaaatga ttaagagtaa aggaagtaag taagttggaa agctgaagat
    20881 ggaaattgga agatggaagt aaatagaaat catatttcaa gtcgctttta ccctttataa
    20941 ctttattaca ttgttaacaa aattccattg gcaaagccat aaataattgt atttttacac
    21001 ctcaaaatct actaaaaaca aatgggtaaa atcattgcta ttgccaatca aaaaggtggt
    21061 gttggaaaga ctacgacttc aatcaatctt gccgcatcac tgggagtatt agaaaaaaaa
    21121 gtgttattaa ttgatgccga tccgcaagcc aatgctacat cgggattagg tattgatgta
    21181 gaaagtgttg aaattggaac ctatcaaata ttagaacaca gctatactcc aaaagatgct
    21241 attgtagcaa gtacttcgcc aaacgtttgg gtgcttcctg cacacattga cttggtggct
    21301 attgagatag aattagtcga taaagataag cgtgagtaca tgcttaaaca agcattggaa
    21361 gaagtaaaag acgattatga ttatattctt attgattgtg caccatcgtt agggttatta
    21421 acattaaatg cgttaactgc tgctgatagt gtgattattc caattcaatg tgaatatttt
    21481 gctttggaag gtttgggtaa attattaaat actattaaaa gtgttcaaaa aatacacaat
    21541 ccagaattag acatcgaagg attactttta accatgttcg attctcgttt gcgtctttcc
    21601 aatcaagtgg tagaagaagt acaaaaacat ttcagcgata tggtttttaa atcgattatt
    21661 caacgaaacg taaaattaag tgaagctcca tcttttggag aaagtattat taactttgat
    21721 gcaacgagta aaggtgcgag caattacttg agtttggcaa atgaagttat tcaaaaaaac
    21781 aataattaga aaatggcaaa agcggttcag aaacaagcat taggaagagg tttatctgct
    21841 ttattaaaag atccagaaaa cgatataaaa tcggttagcg ataaaaacgc cgacaaagtt
    21901 gtaggtaaca ttatagaact ggaaattgat gctatcgaaa tcaatccatt tcaacctaga
    21961 actaatttca acgaagaatc aataaaagaa ttagccagtt cgataaaaga attaggcgta
    22021 attcaaccca ttacggttag aaaattagac ttcaataaat accaattaat ttcgggagaa
    22081 cgaagactta gagcttccaa agtggttgga ctaacaacta ttcctgctta tattcgtatt
    22141 gcaaacgaca acgagtcgtt agtaatggca ttggtagaga atattcagcg tcatgattta
    22201 gacccgattg aaattgcact ttcttaccaa agattgattg atgaaattca attgacacaa
    22261 gaacaaatga gcgaacgcgt tggtaaaaaa cgttcgacga ttgccaatta tctacgttta
    22321 ttaaaattag acccaatcat ccaaactgga attcgtgatg gcttcatctc tatgggacat
    22381 ggtcgtgctt tgatttctat tgaaaactta gatgttcaat cggacattta tcataaaata
    22441 gtgatggaaa acctttcggt tcgtgaaact gaagctttgg tcaaaaatca tcaagacagt
    22501 ttaaaaccag caccagcaaa atctaaaaaa acgaacaatt tttctataga tgaaagtcaa
    22561 aagaaagaca ttgctgcttt ttttggtgca aaaatagatg taaaagttgc cggaaatgga
    22621 aaagggaaaa tcactattcc gttccattcg gaagaagatt taaaccgcat tattaactta
    22681 cttactaaat agtgaataaa gctttttaca tagttttatt ttttttccta cttgggtcac
    22741 aaacgttttt tggacaagtt agcagtagtg atttagtaat agcaaaagat actgcaaagg
    22801 tttcaaaaac gatagtttat gatccgttgc gaccatcaaa agctgctttt tactcagcac
    22861 ttcttccagg attagggcaa gcctataata aaagctattg gaaaattcca attgtttatg
    22921 gtgccattgg aacgagtttg tatttctatt tcaacaacaa taaaaaatac catagttatc
    22981 gcgatgccta caaacaaaga cttcaaggac tgcccgacca atacgattat ctagacgacg
    23041 accgattaat cagagcgcag cgtttttacc aacgaaatag agatttgtcc ttattagttg
    23101 ccgtaggttt ttatgtgttg aatattgtag atgctaatgt tgatgcgcat ttaacgcagt
    23161 ttaacgtcaa cgacaatctt tctatcgttc ccgatttaca acaaagcgat ttcactgttc
    23221 taccaaatct tggattaact ttaaattata agttttagat gaaaatagca ttacttgggt
    23281 atggaaaaat gggacacgtt atcgaacgaa ttgctttaga acgtggtcat gaaatagtgc
    23341 ttaaaaaaac aagttccaat tcttttgatg gtttagaaaa agccgatgtt gccatagatt
    23401 ttagcattcc aactgttgct gtagaaaaca tatcaacagc aatcaatcat ggaattcctg
    23461 taatttctgg aactacaggt tggctagaaa attatcatga aatggtaaaa ctttgcgaag
    23521 aaaagaatgc cgcatttcta tatggttcca attttagttt aggcgtaaat ctgttttttg
    23581 aattaaatga ctatttggca aaaatgatgt caaaattcaa agaatacaat gtttcgatgg
    23641 aagaaattca tcatacacag aaattagaca aacctagcgg aacagccatt tcactagcca
    23701 atgccatcat caatcatacc gataaaaaca attgggcaat tgaaaaccca acacccgaag
    23761 ctgtttttat tgatgtaaaa cgtgaagaaa atgttcccgg aacacattcg gttctttata
    23821 cttctgaagt tgattatatc aaaatagaac atgtggcaca caatcgcgaa ggttttgcct
    23881 tgggttctat cattgctgcc gagtggatta ttggaaaaaa aggcgttttc aacatgaaag
    23941 acgttttgag tttaaactcc aaataacaat ttaaaaaata aaataatttt tgtaacaaaa
    24001 gacgaaactt ataaacttat aaccaaataa ccttcaaaac acttcaacat gtcaatatat
    24061 caatggtttg tatttttatt aatactacaa ctgattcacg gattaggaac ttggaaactt
    24121 tacgaaaaag caggtagaaa atcttgggaa gcttttattc cagtgtacaa ttcgattgtt
    24181 ttaatgaaaa tcatcaaccg accaacttgg tggacattat tattattcat tccaattatc
    24241 aacttgataa tgattcctgt tatttgggta gaaactatga gaagttttgg caaaaactcc
    24301 acagtcgata cttggttaac tcttttcacc tttggatttt atatttatta tgtcaattat
    24361 acccaagatg taacccacat taaagaccgt aacttagtag ccactaccaa agttggtgat
    24421 acggttagtt cgttattatt tgcagtggtt gttgccactt tggtacatac ctatgtaatg
    24481 caaccttata ctattccatc atcctcgtta gaaaaatcgt tgttgattgg tgatttcctt
    24541 tttgtgagta agtttcatta tggtgctcga attccaatga cagcggttgc tgcaccaatg
    24601 gttcatgata cgttgccatt tatcaataca aaatcttatt tatacgacga taacgatgct
    24661 acttcttgga aaaataaatt gcagtttcca tattttcgtt ttcctggttt tcaagacatt
    24721 aaaaacaatg acatcgtagt gtttaattgg cctagggata ccttatttaa tatgtataaa
    24781 aatgccgaca gacgctacga taaaccgatt gacaagaaaa ccaactatgt aaaacgttgc
    24841 gttggtattc ctggtgattc attgcaaata aaagatggta ttgtatatat caacggaaaa
    24901 gaattgattt tacccgaaag agcaaaacct caatattcct ataaaattgc tgttgacgga
    24961 aaaacacaaa tcgatttaga atacctctta aaagacatgc aagtaaccga cccagccgga
    25021 atgattgcgc cagacacatt gtttgtacaa tgtctaactt ttgcaaatgc cgaacgtttt
    25081 aaaaatattc ctggagttac ttcggtaaaa agagtgatta acaaaggtcc agaagacggc
    25141 gttttccccg atatgaaaga cggaaaacca tcggttacta ataactggag tggtgataac
    25201 tatggcccga tatacattcc gcaagaagga aaaacagttg ctttaaacaa acagagtttg
    25261 cctttttata aaatcattat tagcgaatac gaaaaaaatg atttgaaagt tgttggcgat
    25321 gacatctata tcaatggcga aaaagcaact acttatacct tcaaacaaaa ctattattgg
    25381 atgatgggag acaatcgtca taactcatta gacgcgcgct attttggcta cacacccgaa
    25441 gaccacattg taggaaaacc agttttctta tggatgagtt gggatggtca cgctaaagga
    25501 ttcaataaaa tccgttggga acgattattt accacagtta atggcgaagg acaaccacaa
    25561 tcgtacttca aatatttctt aggattattg gcgttatatt ttgttggaga atatttttgg
    25621 aagaaaagaa aagaaaaaca agagtaaaaa aaggttaaag tttaaaaata gtttaaacaa
    25681 tattaaacag tttttaaacc accttaaatc tttaaacaat tttaaacttt tgaacataat
    25741 aatccatccc agctatttcc cttctattag tcattatgtg gcgatggcaa attcggattt
    25801 ggttacgttt gaaatggatg ataattttca aaaacaaacc aaccgaaacc gaatgtacat
    25861 ttatagtcca aacggaattc agttgttaaa tgtaccgatt aaacactcca aaacagtgca
    25921 tcaaaaaaca aaagatgttc agatagaaaa tgattttgat tggcaaaagc aacatttcaa
    25981 atcgttggaa gcagcctata gaagttcgcc tttttttgaa tattttgaag atactatcca
    26041 tcctgttttt gaaaaaaaac accagttttt aatggatttg aatctgcaaa cgatggaaat
    26101 tgtatccaat tgtttaggct ttgaattcga ttataacgaa accaccgaat acttttttga
    26161 tgtagcagat aaaaccgatt ttcgtccatt ggtaaacggc aaaaaagacg tgtcgcaact
    26221 agcatcctat acacaagtat ttggcgacaa acacggtttt ataaacaact taagtattct
    26281 tgacctattg tttaacgaag gaagatatgc ttttgaatat ttgaagcaac aacagttgta
    26341 acaactccaa tttacatctc gaaaaataaa ttttaagtat tgagagcctt tcggcaagag
    26401 acggaagacc taaatacaat tgtgagaacc tttcggcaag tgacggaagc ttctcaatgg
    26461 ttttggtgga ttattttcta gaaaaataag ctgctgctaa ataactttgc tattagaaac
    26521 agtaaaagaa tgttagtctg caaacgaaag tcgcgtcatg attggaaaat gatccgattt
    26581 gttgaagctg gagaaatttt taaaccattt caccttcatc tttttgtcgg caaagatata
    26641 gtctattctg gctggataat atttaaaatt ataggtttgt ccaaaaccat ttccagcttc
    26701 ttcaaaacag tcatttaaat cgcctttgat gtttctatag atgtatgaaa acgcactatt
    26761 gttcatatcg ccacaaataa tcattggata gggacaatct tttttgtggt tagaaaaaat
    26821 ctctgtttga aattcttgtc tcttgaaagc atctcgtaat cttccaaaaa ccatctgtga
    26881 tttttctttg tttatggttt ccacgttatt tgaaatctcg tttacatctg gagaaatttt
    26941 tatcgactgt aaatgcatat tgtaaacgcg aagtgtgtcc tttcctttct taatatcggc
    27001 aaaaatgatg ttgtttccat cttgcaataa gtgaagattt ccttgattaa atataggaaa
    27061 tttagaaaaa atggcttgtc ctgttttaat tttgttgcct tcaataaaaa tggctttata
    27121 tttataaact ctcaaatcaa tttttgcatt ttcggaatat tcttgaatac aaataatatc
    27181 agggtttttt tcgttaacaa atgctaaaat atctttgcca acattcttgt cgtttatcca
    27241 ttcaaagaga ttaaacaaac gcacgttata actcataacg gtgaaatctt tttcgttttt
    27301 aggcaaattg gttgacgaaa gtttataaaa tttatttata aatgtaatcc ctattaacaa
    27361 tacaattccc gataatacta gttgtctttt gaattgtaac aaccaataaa tgaaaaataa
    27421 tccgttaaga attaagaacg agggtaaaat aagtgttaaa accgaaagta atgggaatgc
    27481 tttaggtgcc aaaaaaggta aaacataagc cacaaaggta agcagtatca aaataccatt
    27541 taatacatac atcactttat taaaccacga taactttttc aaattgtgct atttacccga
    27601 ttgaaataaa aattcttttt cttctttggt caaactatca taacccgatt gactaatttt
    27661 gtctaaaatc tcgtcaattt gttgctgggt tttgtctttt atgaccactt tactttctcg
    27721 aattggggtt tttctttgag atgttttgtg tactttctta aaaggcgttt tttgtttctt
    27781 gtcaaatata gttgaaaaaa agttaatagt attggttact attacactta aatcggtgcc
    27841 gttttggagc aatttaatat acaaaaatcc aaaaaaagca ccactcaaat gtgcaatatg
    27901 accgccaata ttgtcaagtt gaatttgcat taaatccaaa ataagaataa ctgccgtgat
    27961 gtgccagagt tttacgtttc ctatcaataa caaacgcact tccatcaacg gttgataggt
    28021 tgttgatgct actaataatg ccataattgc cgccgaagca ccaaccatta gcgatgattt
    28081 tcctaataaa taaaagctca acacaaagat gatactagca aaaatagcac tcaaaaaata
    28141 caatccgagg tattgttttt gggtaaaaaa tgtgagaaac aatcggctgg taaagttcaa
    28201 aaccatcatg ttgaaaaata tatgcagaaa tccataatga aaaaaagcat aggtcaataa
    28261 ggtccatggt cttgtgacaa gcacatcggg atttgaagaa accgctaacc aatttgggta
    28321 ttcaaaaaca cctgttttaa attggtaaaa caaaataata gacaacagat aaatgccgac
    28381 attccaataa atcattttat tggcaacgcc acctattttg tactgtagtt ttatatcttc
    28441 aataatgctc attaatccca acggttttta ttaaattgat tttttttcca aatccacatc
    28501 ataataaaac ctgttattgc gccaccaatg tgagcaaaat gtgctacacc tgtaccacca
    28561 cttccgaaaa gtgaatttcc tttcaatcct aaaaataaat caacggcaat taatcctggt
    28621 acaaaatatt tcgctttgat aggaattgga ataaacataa tacctaattc agcattagga
    28681 aacataaatg caaatgccgt taacaatcca tagatagctc ccgaagcacc caatgatggt
    28741 gtttgaaatt ctcgaactgc agaagctaaa gtgttaaatt gttcttgtgt acactgtgca
    28801 ttatctaaaa tcgttttcac ttttccttga agcatttgtc ccgaagaatc aaacactgtt
    28861 gaaaaatcag tgttcaataa aaagtgcata tcgctttgag atatatttaa tgaagaaagt
    28921 tcgctaagta tggagtgaat ttgaaagtaa ctcacgccaa gatgcaacaa ggctgctcct
    28981 agtccgcaag aaatgtaaaa gaaaatgaat tttttggcac cccaaaaatg ttccaatgcc
    29041 gaaccaaaca ttaccaaacc aaacatattg aatattatgt gcatcaagtt gggcatcgga
    29101 gcatgcataa acatactcgt caaaggttgc caaaaacgaa acaaatcatt ctctggataa
    29161 tacaaggaaa acatcgggta tgccaatggg gcaatctgcg aaccgatgaa aaagataata
    29221 ttaataatca ataactgctt aacagtttcg gtcatgcgca tcataagcta aattttttat
    29281 ctaaatcttc cacactcatt gtgatgaaag ttggtttttg aaaaggcgaa acatttggct
    29341 ctctacaagc aaaaagattg ttcaccaagt tatcttgttc tttttcggtt aaatacgttc
    29401 cggttttcac tgctaaactc tttgccatcg attgggcaat gctatcattc tgcgaaaaac
    29461 tatcgcctcc tactccattt tggaaatccg tcaataaatc atcaaaaata attgaaactt
    29521 gactttcggt aatggtaact ggcaatccag aaatgattaa actatcggta tggatactgt
    29581 cgaaaatgaa tcctaaattt tctagtgata atttaatctc tttaatcact tcaatatcat
    29641 tcacagaaaa atataattct aatggaaata ataatcgctg actagaagca tgatgaacag
    29701 tcatattcgt caaaaactct tcatacaaaa ccctttcgtg agcacgtttt tgattgataa
    29761 tcacgatacc cgactttata ggcgaaacaa tgtatttctt atgaatttga taggttcgtt
    29821 tggtttcttg ctcggtatcg gtatcttcaa ataatgatga agaaacggca tcggtttcaa
    29881 acgaaatttc agaagattgg gtatccattt cttccagtcc aacgtacaaa ctttcccagc
    29941 tgggttgcag ttcgggtttt ttataattaa aattggttgc cgctttttga ggtttctcat
    30001 cagcaaacgg attgtaactt ctatccactt gaatggttgg atattccgct tctttttgtt
    30061 ggtattgata gggtgtatct aaatttgggt cacgctcaaa atccaaaaca ggtgctacat
    30121 tgaactgtcc taaactgtgt tttacagccg aacgcaaaat ggcatacaac gcatgttcgt
    30181 catcaaactt gatttcggtt ttcgtgggat ggatattgat gtcaatcgtg tttggcggca
    30241 ccgataagta caaaaaataa cttggttgtg caccatcttt gagcaaacct tcataggcag
    30301 ccatgatggc gtgatgcaaa taaccgcttt tgataaaacg atcgtttaca aaaaagaatt
    30361 gttcaccacg atttttttta gcaaactcag gtttaccaac aaaaccatgt atatgaacaa
    30421 tctcggtatc ttcctttaca ggaactaatt tttcgttggt tttgcctcca aaaacattta
    30481 caattcgtgg tctaaaatta gattgtggca aattgaacga ctcgctccca ttgtgataca
    30541 tcgtaaaagc aatgttggga tgtgccaaag caacgcgttg aaattcatca ataatataac
    30601 gcatttctac ggtatctgat ttcaaaaaat tgcgtcgtgc aggtatgttg aaaaacaaat
    30661 tcttcaccga aaacgacgtg cctttgggca acaccgctgg ttcttgtgtt gtgaatttgc
    30721 tgccttctat aatgatatgt attcctaatt cttcttggtc ttgtttggtt ttaagttcca
    30781 catgggcgat ggcggcaatt gatgccaacg cttcgccacg aaaccctttg gtatgcaagg
    30841 aaaacaaatc ttcggcatga cggatttttg aagtagcatg acgctcaaag cacaaacgcg
    30901 catcagtaac gttcattccc aaaccgttat ctatgacttg aattaacgat tttccagcat
    30961 cttttatgat taatttgatg tctgttgctt tggcatccac agcgttttct agcagttcct
    31021 ttactacaga agctggtcgt tgcaccactt ctcctgccgc aatttggttg gcaacatgat
    31081 caggaagtaa ttggataatg ctcgacatag ttagtattca gtgttcagta ttttgtgttc
    31141 agtttttctg aaaacaaaga aggcaaattt aaggaaaaac tatggtgatt taaagttaat
    31201 cctttcctaa aaatgaaaaa gatttgttat ttcttaaatc gtttttgtga gtaatctaca
    31261 ggttccaaac tcaaatcaag ttatgaaact tcgctaacca agcaaataac ctcgtcaatc
    31321 tagtaaaagt atcgccaacc aagtaaaaaa ctcgtttttc agttttgaat gtttggcatg
    31381 aatttttttg gtctggtttg aatttatttc tgatggtaaa aatataaaag acattctttc
    31441 gagcaccttt tttatagttt acatttccat tgtttttttt cgtaagtagg ccatttttat
    31501 ggcagctatt gccgcttcgg tacctttatt gccatgtttt cctccacttc tatcgataga
    31561 ttgttgttcg gtattatcgg ttaacaaaca aaaaattaca ggcacatcgg tttgcacatt
    31621 taagtcttta ataccttggg ttacaccttc gcacacaaac tcaaaatgca tggtttcgcc
    31681 tttaataaca catccaatgg tgatgactac atctacttct tgggtaacaa tcatgcgttg
    31741 tgcaccataa atgagttcaa aactgcctgg cacatcccaa cggatgatgt tttcggctaa
    31801 tgcgccacaa tctaacaatg ctgcttcggc tccgttatac aatccgtttg ttattttgtc
    31861 gttccattcg gaaacaacta ttccgaaacg aaaatttttt gcattgggta attttgattt
    31921 atcgtagttg gataagtttt tatttgctgt tgccattttt tagtgttcag tatttagtaa
    31981 tcagtgtaaa aataaaaaac catttttgaa aatattcaaa aatggttttt tatttattta
    32041 tcaatagtag tgaaaaattt taatgtattt aatgctatta gcgcgctaaa taccttaaac
    32101 ttcttactgt gccattccga ttaacccgtc aacaccttgt ccttcaggag tgctttcgta
    32161 gttgtctttt aggtcagtaa aatattttaa tgccgctgct ttatctccaa gagccaaagc
    32221 tgtttttcct gcttttaaca aaaatcttgg tgtagtcaaa tcgtttttct ttgcttcagc
    32281 agctttcaca taataggtca atgcttcttt tgcttgattt ttttgtgaat aagcatctcc
    32341 aatagcacca attgccaaag tactcaatac ttcgtcatct gctttgaatt gctctaagct
    32401 tttgatagct tcatcgtatt tgcctgtgtt taaataagcg atacctgcat agtagtttgc
    32461 aatatttcca gcgtcagttc cagaatattc ttcagcaatt ttcaatacac caaatttacc
    32521 ctcagaacct tttaacacta aattgaataa tgaatctgct tttgcatcac catccacagc
    32581 tttttgaaag ttttgttgtg ctacgaataa ttcattagca gcatcgtctt gttttggctc
    32641 tgcaacaaat ttttggaata ataaataagc aatcgttcct actgcaatta tagctagaac
    32701 acttaatata tatttttgat ttttttgaac aaagtcttcc gttcttgaag ccgtttcgtc
    32761 taatgtatca aacgcttttg ctgttgcgct gtcttttgca tcaacattta cgtcttcaat
    32821 ataattgttg tctaatttgt ctgctttttc ttttggtgtt ctgtaacctc ttttattaaa
    32881 agttgccatt taaaattatt ttagtgtgcg caaaaataaa attttattcc tatttaaaaa
    32941 cgatttttat cgataatttt caataatttg cacgctcttt caaaataaac gcaaaatagt
    33001 tccgcttttt cagcaatagt tagccgttac catgttttta aaaaatatta ctttattcaa
    33061 ttataaaaat ttttcggaag cttcatttga atttgaccca aaaattaact gttttgtggg
    33121 aaaaaatggt gttggaaaga ccaatgttct tgatgccatc tatcatttga gcaatggaaa
    33181 aagctatttc aatccgttag ccgttcaaaa tatcaaacac aatgaagagt tttttgtagt
    33241 tgatggcgaa tttgaaaaaa atgaccgaac ggaacaaatt gtatgcagtt taaaaaaagg
    33301 tcaaaagaaa attttaaaac gaaacggcaa actctacgac aaactttcgg aacatattgg
    33361 tttaattccg ttggtaatta tttcgccagc cgatagagac ttaataactg atggtagtga
    33421 aacacgcaga aaatttatgg atagtgtgat ttcgcaattg gacaaaagtt atctacaaga
    33481 aatcatcaaa taccaaaaaa tcatcttgca gcgaaatgct ttgttgaaat attttgcttt
    33541 aaatcatgtg tttgaaaaag aaacgctaca tatatatgat gagcaattgg atgatttagg
    33601 acaagttatt tttgaaaaaa gaaaatcgtt tttagaaaag tttatcccta ttttcaatca
    33661 ctactatcaa gagataagca acaattcgga aaatgttcaa ttggtctatc aaagtgattt
    33721 gtttgagaaa aacatgctgc ctttattagc tgaaaacatc aataaagaca aagcgttaca
    33781 atacacatcc gttggaattc acaaagacga tttgtccttt gaaattgatg gctttccaat
    33841 aaagaaattt ggttctcaag gacaacaaaa gtcgttttta atcgccttaa aattagctca
    33901 atttgatttt gttaaaaaac aatcgggtga aaaaccaatt ttactttttg atgacatctt
    33961 tgataagtta gatgaaagtc gcgtttccaa aattgtttcg atggtaaatc aggaagaatt
    34021 tggacaactt tttatttcgg atacacatcc cgaacgcacg gaaaatattg taaaagaaac
    34081 gcaccagagt tataaaatat ttaaccttta aaaaaggtat tttttgtatt ttagcctaaa
    34141 attaaagaat catgaaatta aaaatctaca cctatttatt tataagtata attctaagca
    34201 gctgccaagg acaacacaca aaaaaagtgg aaactctaga cgttaaaacc tatgccgaga
    34261 aactaaacat tacaaataac ccgcaactta tagacgttcg cacaccagaa gagtatgcct
    34321 cgggaaaaat aaacaatgcc aaaaacatca attggaacgc aaacgatttt gcaacgcaag
    34381 ctgagaaatt agataaatcg aaacctgttt ttgtgtattg caaagttggt ggaagaagtg
    34441 cacaggcggc aaataaacta tcggaaatgg ggttcaaaga aatctacaac cttgatggtg
    34501 gcatcatgaa atggaatgct gctggaatgc aaaaagaaaa atcgactacc tatattggta
    34561 tgactgatga agcgtatcaa aagctaattg cttcggacaa aaaagtgatg attaatttta
    34621 gtgccgtttg gtgtgcgcct tgccaaaaaa tgaaacctta tatccttaaa atgcaagaag
    34681 aattaaaaga ccatgttacc ataattcgat tggatgctga cgaaaataaa agtttgctag
    34741 aaaagatgaa gattgatggc ttacctgttg ttatagtcta tgaaaatggt aaagaaactt
    34801 ggagaaactt aggttttatt tccgaggaag atttgaagaa acacttgtaa acactgattt
    34861 tacaaattat cacagattaa aaacaatttt atgattacaa aagaagcctt gcaattttta
    34921 gcggatttga ttgccaataa caataccgaa tggatgcatg ctaacaagaa acggtatgaa
    34981 aattataaga aagattatca tgcttttata gcttctattt tagcagaaat gaaaccactg
    35041 gataaaacat tagaaccact tgaagttaaa aattgtacat ttcgaatcaa tcgggatatc
    35101 cgtttttcaa aagataaatc accttataaa actaatatgg gtgtttggtt tacccaaaat
    35161 aaaaatcgaa aaaatagccc tggttattac attcattacg aaaaaggaaa ttcatttata
    35221 gctggcggtg tttggtgtcc agaagcaagt gaattgaaac aaatccgtaa agaaattgag
    35281 ttttttcacg atgacttgga tagaatagtt gcaaataaaa acttcaaaaa agagtttggt
    35341 gaaatcacta gagatgaaaa caacatgctc aaaaaagctc caaaaggata tgacccagag
    35401 catcctgcga ttgaatttct aaaaatgaaa agctttacgg catcggcaaa aattgatgac
    35461 aatttattca cacaacccga atttgccaaa acaattgctg aaaaattaat tacactcaaa
    35521 ccattgaatg attttttgat gcgagcattg gaaacggaag agtaaattgt aataggcaca
    35581 accttaaacc aaattaaaca accttaaact atcttaaacc attagttaca taatgcaaat
    35641 tcacactaac ttttccttaa aaaactataa tacatttggt attgaagcta aagccaaaca
    35701 atttgttgcc gttcattcta tagatgaatt gaaaacggtt ttagaaaatc acgctactga
    35761 aaagaaattc attcttggtg gcgggagcaa tatgttgttg acacaagata ttgattcttt
    35821 ggtaatacac attgatttga aaggaaaaaa aattctgaaa gaagacgacg atttcgtttg
    35881 ggtagaaagt caagcaggcg aaaattggca cgaatttgtt ttatggacga tagaccaaaa
    35941 ttttggtggc ttggaaaaca tgtcgcttat tccaggaaat gttggcacaa cgccagttca
    36001 aaacattggt gcctatggtg ctgaaattaa agacactttc gtttcctgtg aagcgatgga
    36061 aattaccact caaaaaatga agtcattttc taaagaagaa tgtcgttttg gatatagaga
    36121 aagtattttt aaacacgaag tcaaagacaa atacatcata acttcggtga tttttaaatt
    36181 aaccaatcgc aatcataaaa ttagtgtgga atatggtgat ataaaagcag agttagccaa
    36241 aaacaatatt gaaaaaccgt ctttaaaaga cgtttctaac gctgtaatta ctatccgaaa
    36301 tagtaaatta cccaatccaa aagaattagg aaacagcggt agttttttta aaaacccaat
    36361 actatcaaga gaacaatttc taaaagtaca agaacaattt cctgatgtaa aacactttga
    36421 catatctgaa actgaagtaa aagttcctgc tgggtggcta attgaacata gtggtttaaa
    36481 aggctaccga aaagacgatg ctggtgttca taaaaatcaa gcgttagttt tggtaaatta
    36541 tggaaaagca actggtcaag atattttaag tttatcgaaa tacgttcaaa aaactgtttt
    36601 tgacaaatac ggaattgcga ttgaagcaga ggttaatgtg atataaatgt ttttattgtg
    36661 tataaacttc actattataa aatgaaaaaa atattagaaa cttctcgtac ttatcttcgt
    36721 gaattatcta ctgatgatgc agaaaatttc tttttattga attctgacaa agatgtttta
    36781 aaatatacag gcgacaaacc atttgaaagc attttgaaga caaaagaatt tcttaagaat
    36841 tacaatccat atcaagaatt tggttatggt cgttgggctg taattgacaa atcaaacaat
    36901 gagtttcttg gttggtgtgg cttaaagttc acaagagaat taaatgaagt tgatttagga
    36961 tttcgttttt tcaaaaaaca ttggaataaa ggatatgcct ctgaaacagc caaagcttgt
    37021 gtagattatg gttttgaaaa actaaatctt actaaaattg ttggcagagc aatgaaagaa
    37081 aatagtgctt caataaaagt attagaaaaa gctggaatgc aatttgttga tatcatcgat
    37141 tttgaaaaac atgaaggagt attatatcaa ttaaaaaaac aataaatgta cattccagaa
    37201 atatatttaa acgaaaatca agcagaaatt gaacaattca ttcacgaaaa tggatttggt
    37261 attttaatca atcaaactaa agggaaactt tgggcaacac acattccttt acttttagaa
    37321 aacaaaaatg ataaacaaat tttagttgga catatttcca aacttaatcc gcaagcagac
    37381 agttttaaaa ctaatgatga tgttttggca attttttcgg gtgcgcattc ctatatttca
    37441 tcatcttggt atgaccatga aaatgtaccg acttggaatt atcttgctgt gcatgtttat
    37501 ggaaaagtaa agcttcactc atacgaacaa tctgttgagg ctttgaaaag tttagttgac
    37561 aactatgaat tgaaatcaaa aaatccggtt aaagtagaaa atctttctga aaaaactatg
    37621 cgtgaagcac atggaattgt ctcttttgaa atagagatta cccgtattga atcggtcaaa
    37681 aagctttctc agaatcgtga caataaaaac tataaaaaca ttatttctga gctagaaaaa
    37741 accaatgaaa atcaatcaat tgaagtagcc aaagcgatga aaaaaaacag accttaaatt
    37801 gttaaaattc cttttgataa ttgcataaag aataactaca tttgcacacc ttttaaaaaa
    37861 gtagaaaagc attatgttac tatccctatt ttgtttgttt gcagcagttg tgttgataca
    37921 gtttttctat tatatcatcg tttttggcaa attttctttc ggaaaacctc aaaacgaaac
    37981 acctaaaaaa atccctattt cagtaattgt ttgtgccaaa aatgaggaag ataacgttag
    38041 aaacttcatt ccacttttag cggaacaaaa ctatcctcaa tttgaaattg tcttgattga
    38101 cgatgcttca agcgatacta ctttggatat ttttgaagag tttgaaaagc aatattctaa
    38161 tgtaaaatta gtaaaagttg aaaacaacga agccttttgg ggaaacaaaa aatttgcact
    38221 aactcttggt atcaaagcag ctaaatatga atacttgctc tttacagatg ctgattgtta
    38281 tcctacttca aaagattgga ttactaaaat gagttcgcag tttacacaac aaaaaaacat
    38341 tgttttgggt tatggtgctt atgaaaaaat agccaattcg tttctaaata aattaatccg
    38401 atttgaaact cttttatcat caacacaaat ttttggatgg acgaaacttg gaaaacctta
    38461 tgctggtgtt ggacgaaata tggcatataa aagagaagaa ttttttaagg taaatggttt
    38521 tatagaccat atgaaaatcc gttcgggtga cgatgattta tttattaatc aagcagcgaa
    38581 taaaaacaat acaacaattt gttatttacc tgacagtttt acatattcta aacctaaaac
    38641 gactttcagt gaatggttta cccaaaaacg acgacacgtt tcaactgctg gccattacaa
    38701 aggatttgat aagatacaac ttggattgtt ttacagttcg caacttttat ttatactctt
    38761 gccaatcgtt ttattagcat ttcaatatca atggattgct gttgttagca tgataggttt
    38821 tcgatatatt tttacttgga tttctatggg tttttctgct ggaaagctaa aagaaaaaga
    38881 cgtaatctat tggtttccag ttattgaaat tattctaatt tttgttcaat taaatgtttt
    38941 catttccaat acctttaaaa aaccagtaca ttggaaataa aacaatatat agaaaatgcc
    39001 aaaaatggtg atcaagtagc gtttactttt ttgcttgaca actattggaa tgaagtttat
    39061 ggatttatgt taaaacgtac tgaaaatgaa actgattcgg aagacattac catagaaaca
    39121 ttttcaaaag catttgataa aatcgccacc tataatcctg aatttcaatt caacacttgg
    39181 ctgattgcta ttgcaaaaaa tgttcacatt gatatgcttc gcaaaaaaaa gtcttccttg
    39241 tttgttgata tttctgagaa tgatgacaga caagcctata atatagctga tagttcgcca
    39301 actgcagaag atgaattaat taccgaacaa aacttatcac aattactacg ctatataaaa
    39361 gaattaaaac ctcattatca agaagttatt caacttcgtt attttcaaga aatgagttat
    39421 caagaaattt cagaacaact tgacgaacct ttgagtaacg ttaaaatcaa acttcttcga
    39481 gccaaaaaac tattggcaga aattatcgaa aaaaaataat tttaaaaata attacttttt
    39541 tacacaataa aatactattc ttttacgtta tctattaaag aaccattact tatgcaacaa
    39601 cgatacctaa caattaaaaa ctatttatta tgtcaaacgt tagtatctat ggaaaaaaat
    39661 ggattgattt ggtgtttgaa gaccgaaacc aaatgtatgg tgcctatcaa ttgcgccaac
    39721 agaatccgaa aatgacactg ttagcacttt tttatggggt gttattaatg gcaggattta
    39781 tgagtattgc actctactcc aactttagat cggaagaaat cagcattacc gatattattc
    39841 cagaagaaac aattattcaa ttatcaaact acagcgaacc agttcctgaa gtagaaactg
    39901 ttgtaaaaac tacaccacca aatacggtta ctgagccaat tgttgaacct gaaattaata
    39961 gattttcgca tttaacagtt agtcaaagta gtgaagcagt tgtaaaagtt ccaacaaatg
    40021 aagaattgaa tactgttgaa acgccttcaa ttattggaac tactgctatt gttggtagtg
    40081 gaatcgaacc aggaactgat agtggaaaaa caaatggcaa tttacttgga aatgaaggca
    40141 ataactctac aaacaatacc ccaagtaaag catcagaagt agataaacta cctgcatttc
    40201 ctaatgggat agaaaatttt tataaatatg ttgcaagcaa tttcagaaat ccagacttag
    40261 aaagtggcgc aattgtttcg gtattagttg cctttgtagt tgaaaaagac ggtactttat
    40321 ccaacattag agttttaaga aattcaggtt ttggagttga taaagaagcc ataagagtac
    40381 taagtgcact aaaaacaaaa tggtcgccag gaattaagaa tggtgaaaag gtaagaactg
    40441 agtttacgct tcctataaaa gtaaaattta attaaactga gaaaatccgc atctagcgga
    40501 tttttttatc ttactattat cttaaaataa gtctttcctt ttttattatt aaataattaa
    40561 tagtattatt gtaaagtaag taaaccacat gaaattaccc gattgattta tgcaaatgaa
    40621 atttttaaca gtaacttttt tttctatagt attattatac ttttcttcgc caaaacaaca
    40681 ttcttttgtt ttaaaaagtg aagagatacc atctgacagt tctaaaataa gtattccata
    40741 ttttttaaaa ctaggaaaag tttcagattc ttttattgca gagaaaagta aaaaaataga
    40801 ttcattttac atcaaaaaca taggtacagc tggctattat ggaagtttta tagtagctaa
    40861 aaacggtaaa attatttatg aaaaatacca aggaatggct aatttaaaac tacaaaaacc
    40921 gatggatgaa aatagtgcct tgcatttggc atcggttagt aaagttttaa cggctacagc
    40981 tattttaaaa ttggtagaaa atgacaacat catgttggat caaaaagtta ccgattggct
    41041 tccaagtttt ccgtataaaa gtacaacaat tagaacgtta cttaatcata gaagcggatt
    41101 gcaacattat tcaaggtttc cagaattgat gaaaaaaaac tggaacagca aaaaaatact
    41161 ttctaataag gatattttgg aattgatggt aaaaaataaa tttcgcttaa tttcacccga
    41221 taatactcgc tttgattatt gtaataccaa ctatgtgatg ttggcattaa tcattgaaaa
    41281 agctactgga ttgtcctatg gtgaagcaat gaaaatgctt attttcaaac cattgaacat
    41341 gaaaaacact tttgtatttg actattttag agataaagat actgtttcga tgtcatatag
    41401 aaacaataag gcaaatcctt gggatcaatt tgataatttg aatggtgaca agaatattta
    41461 ctcaacgcca agagatttag ttaaatttga tttggcaact tattctaatg attttatcaa
    41521 tcaaaattta atgaaagaag cttatgttgg ctatacttca aacatgaaac ttggaaaaga
    41581 ttatggtcta ggaatgcgta tgaaaaaatt agcaaatggt gaaacacaag tctatcacaa
    41641 tggttggtgg catggaaata cttcgtcatt tgttccagta aaaaaagata ctatttcgat
    41701 aatttgtttg tcaaacaagt attcaaataa tacttatgaa accttgaaat tggtagaaag
    41761 tttatatcct caaaccaaat aaattccgtc ttctttaatt tcgataattt tatctttata
    41821 aagcgagcct actgcttttt taaacgattt tttactcatt ttcaacaccg ttttaatatc
    41881 ttctggatga ctattatcgt tcaatctcag aaaacctcta ttcgctttta gctcttttaa
    41941 aatcacttcc gcatttggct caatattctc aacaccaatt ttttggaatg aaacgtctat
    42001 tttattatct ggtcgaatcg ttttaatgta tcctttatgt ttatctccaa tacgcaaatc
    42061 ttcataaatt tcattactgt acatcaaacc tttatgtttt ttattgataa tgacatttgt
    42121 tcccaaatca gtaatgtgtg aaataatcaa atcaacttca tcaccttgtt gtaaatcaag
    42181 ttcggagtta tctaaaaact tgtttactct actggagcca acaattctat ctgatttatc
    42241 atctaaataa gcatgaacta aatatctttt cccttgttcc atagggcgcg cttgctcttt
    42301 aaatggaaca aacaaatctt tttctaaacc ccaatcgaga aatgcgccaa aactattggt
    42361 gtaatttaca cgaagaaatg caaagtcatt tattataata tatggttcga gcgttgtagc
    42421 tacaggtcgt tcttcatggt caagatataa aaacacttta atttcatcac caattttata
    42481 gtcttttggg atgtatttta atggcaataa aacatcattt gtattatcgg ttaaaaacaa
    42541 tccaactttg gtaagtctat caattattag tgtattaaat tctcctaatt tcattttata
    42601 taacggtttg aaacgattca acagcaaaga taaacaatct gtcttcaata ctgataaagc
    42661 attcatttaa taaaactaac tttgaccttg taacaaaatt aaacaagcaa ctactaattt
    42721 gtcatgagcg aaactttaga gcaatctttt gtacaacaat taaaggaaaa tcagaatata
    42781 gttcacaaaa tctgtcggct atatactaat gattctgatg ctcataatga tttgtttcaa
    42841 gaaattacga tacagttgtg gaaagcgttt ccaaaattta gaggtgattc taaattctca
    42901 acatgggcat atcgtgtagc tctaaatact gccattacac tttatagaaa aagcacacgt
    42961 tccgtttcaa ccgtttcgta tgaagttcaa aaacattttt ttgctcatga agaatacaat
    43021 tatgaagaag aagaaaaatt gaaattaatg tatcaagcag tacatttatt aaatgatatt
    43081 gaaaaagcac tggtttacat gtatttagaa gacaaagatt atagtgaaat agctgaaact
    43141 cttggaatat ccgaagtaaa tgcacgtgta aaaatgaata gaattaaagg aaaattaatt
    43201 aaaatattaa atccctaaag aaatatgaat gagttggatt tattaaaaaa agactggaaa
    43261 aaagaaaacc caaactatac tcaagtatct gaatttgata tttataagat gttgcacaaa
    43321 aattcttcat caactgtaaa atggatattg attattggta tagtcgagtt cattgtatgg
    43381 acaggaattg gtttcttttt caatacagat gactatgtaa aaagtattca tgctgaggaa
    43441 atgatgattt atttaaaagt actaacctac ctaaattatg cggtagtgct ttggtttatt
    43501 tataaatttt atatgaatta caagaatatt tcaacaatgg tttcaacaaa acaattgatg
    43561 aaagacatta tgaaaactcg aaaaacagtg aaccaatatg ttgcttataa tttagccatg
    43621 atagttgtca gcatgatttt aggatttatc atggcgtttt tatacaatcc aaaactatcg
    43681 gttataaagg aaaaaattgc aaatgatgcc agtaatggag ttttattaaa aatcattgga
    43741 ttattaacat tagccatagt tatttttgtg gttatctttt ggttattcta caaattactc
    43801 tatggaattt tgttaaaaag actaaaaaca aacttcaagg aacttcaaaa aattgaaatc
    43861 taacatgaaa aaaatagcct tattatttgg tctttttttg gcactattca gtaacgctca
    43921 aaccgaaaaa aaatcattac tttggaaaat ttcaggaaat ggattagcca aagaatccta
    43981 tttatttgga acaattcata tagcatgcga tgccacttta cctaaaaaag ttttggatgc
    44041 attggataag acaaatcaac tttgtttaga gttggacatg gatgacccca acatgcaaac
    44101 tgaaatgatg ggcaaaatga tgatgaaaaa tggcgtaacc attgaaagta tgatgagcga
    44161 agacgacttt aaattggttg atgatttttt caaaaaacaa tctggttttt cattaaaaat
    44221 gatgaattcc attaagccat ttgctttgag tgctatgttg tatcccaaaa tgttggattg
    44281 tccgatacaa tcttttgaaa atgaattgat gaaagtagcc aaagaacaaa acgaagaaac
    44341 aattggatta gaaacaatag gcgaacaact tgccatattt gatgctatac catatcaaac
    44401 acaggttaat gaattgataa aatctgctaa aagtgattta tcaacagata aaaaagaact
    44461 agaagcattg cttgaactct acaaatcaga agatcttgaa gcaatgttgc aatattccga
    44521 ttcttccgaa aacataatga cctcagaatt taaagaagaa cttttaaaca accgaaataa
    44581 aaattggatt gatagaatca caaaaattgc tatggaaaag ccaacttttt ttggtgtagg
    44641 agcagctcat cttggtggag aaaatggcgt aataaaatta cttagaaaaa aaggatataa
    44701 agtaatagcc gtttattaat cgatcgtttt ctttggttgc cacttacgca atttattcag
    44761 tctttcttgt tcttcaattt cttcagcagg aataactttt agaaatgacg gatgttgctc
    44821 tatggcgaat tctatctttt ctacaattgc atcaatcgaa tcattttcat aatcaatctc
    44881 caaaggttct tttatgataa aagattgcaa tatatttttc ttctttacgc gtaatccttt
    44941 tttatcaaaa gctctacgaa atccatcgat aactattgga acaacaatag gtttgtgttg
    45001 cttaatgatg tgagctgttc cttttctaac tggtttaaat gattttgttg ttccttgtgg
    45061 aaatgttata acccagccat cgtctaaagc aattttgatg ttttccgtat catttggatt
    45121 cacttctttt ttttcttcaa catctttccc tccagcacgc catgttcttt caacagtaat
    45181 ggcaccaaca taagccataa ttctcggtaa tattcctgac ttcatggttt cactggcagc
    45241 tacatagtag atattcattt ttggctgcca caaataccct acgttcttaa tagtatcaac
    45301 tctacccgaa agacttgcat taaaaacatg aaacatcgct acaacatcag caaaataagt
    45361 ttgatgatta gaaataaaca atacattttt atctggaaga gatgtaataa tttcagaacc
    45421 ttctatttga agttgattaa atcctcgata ccttctatgt gttaatgcac caaagattct
    45481 aattaaccat tttttgatga aaaggatatg accaaaagga tttcttttaa ataatcccat
    45541 tatattttac attttttaga cttgcaaaaa tagaaaaaca aatggtttac aacactattt
    45601 ttagggtttc ttttaattcg ctcaacatca ttgctgttgc tccccaaaca aaatgctctt
    45661 tgactaaaaa accaggtact tcaaaattag aagcatagga agtattcatt tttttatttt
    45721 gaataattct ttcatccaaa aaatcaacca acggaagttc aattattcca gcgacttcct
    45781 cttcttgtct aacaaaattt aattcctctc tacttattcc taaataagga tgcaccatat
    45841 aattacttgg cggaatatat acttctgtaa acgaacgaat cacttcaatt ttattgggca
    45901 aaattccaat ttcttcatga gtctctctta aggcagtttc ttttaaatca acatcaataa
    45961 gttccggctt tccaccagga aatgcaattt gagacgagtg cacgccttta taagaatttc
    46021 ttaaaataag gaccaaatga gtttgattat tttttggata aaacagcatc attactgctg
    46081 ctttttttgg gttaacatta ttaggattta attttttcaa caaatccatt ctgttagaag
    46141 gtgtcatttt tgcatgagca atggttgctg gtaatcctac atttagtatt tttggcgtaa
    46201 attgtaaaaa attatcaaaa tccatttttc ataatttaaa aacataaagt tacctaaaaa
    46261 agaaatctaa aacacttttc attcaaaaaa atgtatagca aagaagaagc tcaaaaaata
    46321 aaaagacaat tttggattgc atttgcagta gcttatccaa gaaaatggat gctttatgaa
    46381 accaaaatta aagatgtttc gtttaaattt tttgtcgata ataaaaaagc gcaagttatg
    46441 ttagatatcg agccaaaaga agaagaaaag cgtaaaattt attttgaaaa aatagaatct
    46501 ttacaaagta ttttattaga agaccaccta ccagaagcca ttttggaacg taattttcat
    46561 ttagaaaccg gaaaaacaat cagtcgggtt tgggtagaaa ttgacaacgt tagcattaac
    46621 aaccaaaaaa cttgggaaac aatttttgct ttttttaatg aaaagatgag tgcttttgaa
    46681 tattttttct tagaatatga agactatatc aaagatttag aaacaaatac ttaacgaaat
    46741 atcaaatcct taactacatc tttgcgaagt tcttcattta tcattgagat aattttttgt
    46801 tttccataac taagctcttg tctcaaaaca gcagatgata atgaaacata taaggtagaa
    46861 cctttaagaa ttacgtcatt tgtatagctg ttaacaccat ttcccataag tgatttccat
    46921 gcttgttgca catcaatttt gtccatgcct gattgaagct tatttacttc gataaactct
    46981 tttaaaacat cactaattga actttcgttg cttatccttt ttgccattat tttaacgaaa
    47041 acggtttgaa ttttttatag gtatattcta aattttcttt atttttcaat actaaagtcg
    47101 aatcttttaa acttagaatt tcttctttcc acttgccata atcagtggtg tattcaataa
    47161 aaaaagtttt attactatca atgattttta tagattcttt aatttcgttt gttttaaatt
    47221 ttccatcaaa ctgaggcatc actttttgtc taaaccctat agtattttta acttcaaaat
    47281 aatcaactgt ttcattgact ttatactctt ttttctcgcc atcaggttta gtaacttgct
    47341 taatttccca ataaccattt aggttaatta aatcttcttt cttaatctct ttttggcaag
    47401 aaacaaataa aaacgcaatt actaatacta taattctctt cattttactc atataaattg
    47461 atggaaatct tcttctaaac cacaaattta aattttatat ttaagaatca tcctgtttta
    47521 aaattaaaaa tcaattaaac ctttttatat ctttagtgtc caaatgaaaa atgcacaaaa
    47581 tgaaaaaact ttatatttta ctattatttc ctttgttact ttcaaattgt agcgctgact
    47641 ctaatgaaaa tcctgttgaa gaacaaatgt attttccacc aactgatgga agcactacat
    47701 gggaaactaa atctatttta agtttaggtt ggaatcaaaa tgcagtacaa ccgttattgg
    47761 agtatttaga attaaagcat tccaaatcat tcatgataat agtcaatgga agaatagtaa
    47821 tggaaaacta ctttaacggt catacagcaa caacaaattg gtattgggca agtgcaggaa
    47881 aaacattgac agcaacttta tcaggaattg cagagcaaga aggattcatt gatattaaca
    47941 ataaagtttc ggattacatt ggaacaggat ggacaaatgc accgttagct aaagaaaacc
    48001 taatcacttg caaaaacttg ctttctatga attctggatt gaatgatgca ttaggcgatg
    48061 acgtttcgcc taccaatctt caatatactg ccgatgctgg aactcgttgg gcatatcaca
    48121 atgtgtatgt aaaaatgcaa gatgttgttg cgcaatcatc tggacaaaca tggaacaatt
    48181 atttcaatac aaaattgcgc gataaaattg gcatgaatgg tttttggttt aatagtggca
    48241 cttctggcga tttaagtgtt tattggagta ccacaagaag catggcgcga tttgggttac
    48301 ttaatttgaa taaaggaaag tggaatggaa cacaaatttt aaatgaaaat tattttgctc
    48361 aagctactac tacttctcaa aacatcaatc tagcttatgg ctatttatgg tggttgaatg
    48421 gaaaaacaag ttatcattta cctcaaactc aaattcaatt tagcggaagt attattccaa
    48481 atggtgctaa tgatatgttt atggcattgg gaaaaaatga ccaaaaaata tatgttattc
    48541 caagtaaaaa aatggttgtc atcagaatgg gcgatgccgc tgatgatgag aattttacac
    48601 tgtcaaattt tgacaatgaa ttatggggga aaataaatgc tttaattcta taaatttatt
    48661 aaaactttga attaaagaat tttattgttg cataaatcac taaaataaaa gtcaataatc
    48721 ttgcaaaaag gaagatactc ggtaatgatt tttcaacaaa gaatgacaaa attaaaaaag
    48781 gtattgccac taaaatcaac tttaagggcg aaccttcttt gaaaaaagtt gttattttat
    48841 tcatatatta tttaaaaaaa gaatctacaa actcatattt attaaaaacc tgcaaatgct
    48901 caatgccttc tcctactcca atatatttga caggaatttg aaattgatct gaaataccaa
    48961 ttacaacacc accttttgca gttccgtcta atttggtaat cgctaatgaa gtaacatcgg
    49021 ttgcagcagt aaattgtttt gcttgttcaa atgcattttg tccagtcgaa ccatcaagca
    49081 ctaacataac atcatttggc gcatcaccaa caaccttttg cattactttt ttcacttttg
    49141 aaagctcatt catcaaacca atcttattat gcaaacgacc agcagtatca atgattacaa
    49201 catctgcgtt ttgagaaaca gcactttgta atgtatcaaa agctacagaa gcaggatcgc
    49261 ttcccatttg ttgctttaca atcggaactc caactctatc agcccaaatt tgcaattgat
    49321 caatggcagc agctctaaac gtgtcagcag cacccaaaac taccgaataa ccagcattct
    49381 taaattgatg tgctaatttt ccaatagttg ttgtttttcc aacaccattt actccaacca
    49441 ccataattac atagggttta gtgtcttttg gtattgaaaa ctgtgtttca tctccagaat
    49501 tagtttcaga aagtaaagcg gcaatttctt cgcgaagtat tttattcaat tcatctgttc
    49561 caagatattt atcattggca acacgttttt ctattcgttg aataattttt aaagtagtat
    49621 caactcctac atctgaagaa acaagaattt cttctaaatt atctaaaact tcatcatcaa
    49681 ctttagattt tcccgcaact gctttggtta attttgaaaa aaaagaagat ttagattgct
    49741 ctaatccttt atcaagattt tgttttgcct ctacgctttt gctagggtca tttttttcgg
    49801 aagaaaatag tcttttaaaa aaactcattt ttaaattagt ataataatca attaatgaac
    49861 aaatataaaa aataaaaaag ctacttccga aagaaagtag cttagatata gtgagtttat
    49921 caaattattt ttttgtcaag aattcatcaa ctagctcagg agccataatt gattcttgaa
    49981 aaatataagc acctgttttt ggagatctaa ccattttaat agctttggtt aatctctttg
    50041 atgtagtttg taatgttgct accgttttct ttgccatgat tcaaatgtat tttgaagatt
    50101 ttattagaat aaaatattct tattacttaa tttctttgtg aacagtcatt ctctttaaga
    50161 ttggattaaa ctttttaatc tctaatctat ctggagtatt ttttttgttt ttgttagtaa
    50221 tgtaacgaga agttcctgga agtccagatt ccttgtgttc agtacattct aatataactt
    50281 gtattctatt acctttcttt gccatcttac tttatattta tcaggtttgg gaattattta
    50341 ataaatccgt ttgctttagc atctttcaat actgcagata aacctttttt gttaattgtt
    50401 ttaattgtag atgctgctac tctaagtgaa atccatctgt cttcttcagc tagatagaaa
    50461 cgttttttaa ctaagtttac agaaaatttt ctcttagttt tgttcatagc gtgagaaacg
    50521 ttatttccta ccatcgcttt tttacctgta aggtcacaaa ctcttgacat tatgctttac
    50581 ttttttcgtt attcaaaatc agggtgcaaa cttacgcaaa ataaatcatt gaaacaaaat
    50641 aattattaca cattttttaa aatttctttt tgcaataatt caaacgattt gtttacagct
    50701 ctatctatca ctttttcacg aggttgtcca aaattaaact cttcaactat tacttttttt
    50761 ggtgttgaaa tagcaataaa tactgttcct atttcagctt ctgaatcacc tttactaggt
    50821 cctgcgtttc cagtcgttgc aattgcataa tcagaattca ttgttttttg aattccaata
    50881 accatttctt ttgcaactgc tgcgcttaca acagtatgtt ttttaattgt ttcaggattt
    50941 acatttaatt gagtaatttt tgtatccgtg gcgtaagtca caacaccacc cctaaagtaa
    51001 ttggaagcac ctgaaactga cgtaatcatt tgcgctattt ttccacctgt gcaactttca
    51061 gctgttgcta aagttctttt ttgttgcgtt agcaattttc ctaaaacaac ttcaatagtt
    51121 tcatcttcat caaaaccaac tataatatca tttataattt tagttaaggt gtttacattt
    51181 tcttcaattc ctttttgtaa aatctcttta cgttctcctc ttgcagtcaa tcttaaacga
    51241 actcttcctg gacttggcaa ataagctaac tttatgaatt ctggaagatt attttcccaa
    51301 ttttcaatgc gttctgcaac taaactttca ccttgaccat aagttaaaat cgttttatga
    51361 agaataaatg gacgtttata ttctttaaca atttttggaa caatttcatt ttcaattaga
    51421 tatttcattt cataaggcac accaggcaac gaaataaaaa cagttttatc tttcttcatc
    51481 cacattcctg gtgctgttcc cattttatta aacaggactt cacattttga aggaactaaa
    51541 gcctgatctc gatttaattg ggtaattggt cgtttgtaaa aaccttcaat caattcttta
    51601 acatgattaa aaacctgaac gttttcaacc aattcatctt caaaataatc acaaaaagtt
    51661 tttttagtta tatcatcttt agttggtcct aaacctccag taataatgac aaaatctacc
    51721 ttgttttgaa gatttaaaaa agtatctaaa atgtgttttt tatcatctga tattgaaagc
    51781 atttcatggg tttgcactcc tattttatct aaagatttag caatataact agaatttgaa
    51841 tcaataattt gtccaattaa tatttcatcg ccaatggtaa ctatagtagc tttcatattc
    51901 gttatttatt ttggcaaagg tcgttaagtt ttttataaaa agtaaaaaaa taacgaaatc
    51961 gattaagcca ttttattatt tttagtaatt tggctgaaaa tcgactttat gaccttcaat
    52021 cccgaagaat tagacaaatc agcagcttac aaacttctaa ctggaacaat aattcctaga
    52081 cctattggct ggatttcaac cgttgatgaa aatggcaata ataatcttgc tcctttttct
    52141 tatttcaata tggtttcaag tgatccgcca tgtgtaatgt tttctacgcg aagagacaac
    52201 aaccaaaata aagacactct taataatgtt ttaatcacta acgaatttgt tgtaaatttg
    52261 gttaccgttg atatagttga aaaaatgaat acaacttcag cttctgttcc tccagatatt
    52321 gatgaatttg aattagcaaa acttacacct attgattctg taatggttaa acccaaaaga
    52381 gtgaaagaaa gtttagtaca ttttgaatgt gaaaaaattc atcattattt tattgataat
    52441 gattccaatg gtggcgcatg tgttgtaatt ggaaaaatca aaatcattca tattgatgat
    52501 actatacttt tagaaaataa ttatattaac cttgagaaat acaaacctgt agcacgttta
    52561 gctggttcaa attacggaac attaggagaa attttctcta taaaaagagc ctaaaatgaa
    52621 aactaatgaa ttaattatcg atgttgctat gcaaattcaa aagccaagta atgaagtctt
    52681 tgaagccatt gtaaatcctg aaaaaatgac taattatttc atctcagaaa gtactggaat
    52741 gatggaagaa ggaaaagaat taatctggaa atttcccgaa tttgattttg actgtccagt
    52801 tcgaattgga aaaattgaaa aagacaaata cgtttcctat tattggatga tggaagaaaa
    52861 agaactgttc gttgaaataa ctttaaccga aaaagaaaac aattctacat tagtaagcat
    52921 cacagaaaaa agcatgaata atgacgaaaa agggttaaaa tggttatcag gaaactcttt
    52981 tggttggtca aattttttgt cttgcttgaa agcttatttg gaatacggaa taaatttaag
    53041 aaaaggagct tttgatttta tgcgaaaaga aaatcaatgt tcttaaaaca aaacacatga
    53101 aaataacagt aattggtggt ggtccaggtg gattatattt ttcgatttta accaaaaaag
    53161 caatgcccga ttgccaaatt gacgtttatg aacgcaatcg tccagatgac agttttggtt
    53221 ttggagtggt tttttctgat gaaacattag gtgagttctt aaaaaaagac atgaaatcgt
    53281 atgagttgat tcgaagtaaa ttcgcttatt gggacgacat tataattgct cgtgatggac
    53341 aagaagtcag catcgcagga aacggttttt gcggttgttc tcgaaaaaca ttactacaat
    53401 tactacacca acgatgtgaa gaagaaggag taaatcttca ttttgaagcc aatgttgatg
    53461 atttaagtca attttctgat tcagatgtaa tagtagcttg tgatggaatt tcaagtcaaa
    53521 taagaaccca atttgaaagt gatttcggaa caaaagtaga gttaaagaaa aacaaattcg
    53581 tttggatggg ttctacaaaa ccactagacg catttacata tttctttaag gaaaccaaac
    53641 tcggtgcttt tgctgctcat acctatcaat acgaaaaagg tatgagtaca tggatttttg
    53701 aatgtgctcc agaaacttgg gaaaaattcg gatttgatac tttcaatgaa gaagacacgg
    53761 ttcaaaaatt acaagaatta ttcaaaaccg aattagacgg tcatggttta attaccaata
    53821 aatcacattg gaggcaattt cctcatgtaa ccaatgaaaa ttggtatcat aataacattg
    53881 ttttattagg agatgcaaaa gcaacagccc actactctat tggttctgga acaaaattgg
    53941 ctatggaatc tgctattgct ttatccgatg caattattgc aaatccaaat gatattcaaa
    54001 ctgcttttca aaactatgat aaatcaagaa gaaatagtgt agaaatgatt caatatgcag
    54061 ccaatgtttc tttggattgg tttgaaaacc tggacagaca tatacaacat cctttttatc
    54121 agtttgcttt tggatgtatg actcgttcta aaaaagttac ttacgaaaac ctacaaattc
    54181 gcgataaatc ttttacagat aaggttttac atgaattcaa tgataattac aatgtcaatg
    54241 acaaatatca atcagctgca ttttcaactt tcaaacttcg tgatttagtt cttcaaaata
    54301 gaatagtaat gtctccaatg ggacaatatt cagcagaaaa tggattggtt aacgattggc
    54361 atttccaaca ttatgcttct cgtgccattg gaggcttggg attaatttta accgaaatga
    54421 cagctgtttc agaaaaagga cgtattacat taggctgtgc aggtatttat gatgaaaatc
    54481 aaatagttga atggaagcgt attaatgatt tcattcacgc aactacacaa tcaaaaatcg
    54541 gaattcagtt aggtcattct ggtcgaaaag gtgccacaaa aaaaccttgg gaaactgaag
    54601 atttaaagga taaatgggaa ttgctatcag catctgctat tccattcaat gaaaattttt
    54661 caattccaaa agaaatgact ttggaagata tggatttgat aacttctcaa ttcgttcaag
    54721 ctacaaaaaa tgctgacaaa gctggatttg acatgattga attgcaagca catcacggat
    54781 ttttgttggc ttcatttctt tcaccattaa cgaataatag aaaagatgaa tttggtggaa
    54841 gtattgaaaa tcgtttaaaa tttccattgc gagttttcaa tgagatgaga aacacttttc
    54901 caaaagaaaa accaatgtcg gttagaattt cagccacaga ttgggcagaa aacggtattt
    54961 cagaagaaga tgttttgatt attgcaactg cttttaaaaa cgctggagct gatattataa
    55021 atgtttccac aggaaataca gtagccaatc aaaaaccaca aaccggaaga atgtggcaaa
    55081 ctcctttttc agatactatt agaaatacag ttaatgttcc aacaattaca acaggttata
    55141 ttcaagatat tgatcaaata aatactataa ttcttaacgg aagagctgat ttagtagcat
    55201 taggcagacc tttactttta gatacaaatt ttgttagaaa cgcacaagct tacgagaaat
    55261 atcaaactga tgatattcca aatccatata aaatgggaat ttctcattta tatcctttaa
    55321 aagcttcaga aagaaaacaa actgaaggaa tgaagaaagc attgaaacct aagagcaaca
    55381 aaaaataaat tacaaaatgc aaatcccaaa ttccaaattt tacgttttaa ttggaatttg
    55441 gaatttttaa tattggaatt tcaaatatga aacattatac agacaatttc gctcacgata
    55501 atctaccaag tatagactta caacccgatt atttgtgtga tttacctcaa tttcaacatc
    55561 ctgaaatgct gaattgtgtt gaacgtttgt tagacaatca catcaaaaaa gggaatggtg
    55621 atgcagtttg tattcgaacc tttgaagaat catggactta caataattta ttcgaaaaag
    55681 ccaaccaaat tgctcacgta tttattgatg atttaggttt ggtttctgga aatcgagtat
    55741 tgttgcgttc agcaaataac ccgatgatgg ttgcttgttg gtttgccgtt ttaaaagctg
    55801 gtggaatagt cgtttcaaca atgccgttac ttcgttcaaa agaattaacc accattattg
    55861 attgtgccga aatttccatt gcaatttcag atagcgattt ggctgatgaa atgaaccaag
    55921 ttgaatccaa gtttttaaag attacaagtt ttttcagaaa tggagattta gataaattaa
    55981 cacaatctaa acccaaaaat ttcgagaatt atcattcaaa agcagatagt gtttgtttaa
    56041 ttggtttcac ttctggaaca acaggtttac caaaaatgac agctcattac cataaagata
    56101 ttttgaatat ttgcgaagct tttccagttt attccttaca acctacaaaa gacgatattt
    56161 ttattggaag tccaccatta ggttttacat ttggtcttgg aggattggtt ttatttccta
    56221 tgtactttgg agcttctaca tttttaatcg aaaaaccaag tcccgattta ttactaaaag
    56281 ctattcaaga atataaagta accatttgtt tcacagcacc aacagcttgg agggttttaa
    56341 ccacaaaatt gaatgaattc aacgtttcaa gtttgagaaa atgtgtttca gctggggaaa
    56401 ctttgccttt acaagtttgg catgattggt atgatgcaac aggtttaaaa attatagacg
    56461 gaattggttc caccgaaatc cttcatattt ttatatcatc caatgaagaa aacatgattc
    56521 ctggagcaac aggattacct atattaggtt atgaagctaa aatcattgat gaaaatggaa
    56581 atgaacttcc aattaaccaa tcaggaaaat tagccattaa aggtattaca ggttgcaagt
    56641 atttaaatag agaagaaaaa caaaaagaat acgttcaaaa tggctggaat attacgggtg
    56701 acattttcaa aaaagatgac aatggttatt actggttcgt tgctcgtggt gatgatatga
    56761 ttatttcttc gggttataat attgctgcta ttgaagttga atctgtactt ttaacacatg
    56821 aagatatttt agaatgtgcc gttgttggtt tgcccgatga agaacgaggt atgttagttt
    56881 gcgctcatat tgtactaaaa gacaataaca aagcttctgt agaatttaaa aaaacaattc
    56941 aacaatggtt caaacaagta gctgcaccat ataaatatcc acgtgtaatt aattttatag
    57001 agagtttgcc aaaaacagag actggaaaaa ttcaacgatt taagttgaag aagcattaaa
    57061 gaattaagta ttaagataaa tgtatgatta aatcatttaa aacttaaaaa atgagaaaaa
    57121 cctttttagt aattagtttt acactgttat cattaaccgc tattttgact tatatcaatt
    57181 ggaaatttag ctttacactc ttaatcttaa ttccacttat tttaatggga ttatacgaca
    57241 tgtattttgc agacaggaca attcgaagaa attttccatt attgggaaga atgcgttata
    57301 tactatctgc aattggtcct gaaatgcgtc agtattttat tgaaactgat ttggacggaa
    57361 aaccattcaa cagattacaa cgagatattg tgtaccatcg cagtaaaaaa gaaaatgaca
    57421 ctatgccatt tggaacccaa ctagacgtgt atcgggatgg ttatgaatgg attaaccaca
    57481 gcatcagtgc aattccattt gcaaaagtaa atcataatcc aaaagttaaa attggttctt
    57541 cacaatgcac aaaaccttat gatgcaagtc tatttaacat tagtgccatg agttttggct
    57601 cgttaagtaa aaatgccatt atagctttaa acaaaggtgc aaaacaaggt ggattttatc
    57661 ataatactgg cgaaggtgga ttgtctcctt accatttact tgggggagat gttgtttgga
    57721 acattggaac aggatatttt agttgtcgaa aatttgacgg aacattcaat gtagatgaat
    57781 ttgaaaaaag agcgattctc gataacgtca aaatgattga aattaaattt tcacaaggag
    57841 caaaacctgg tcatggtgga attttgccaa aagaaaaagt aacggctgaa attgcggcaa
    57901 tccgacttgt tgaaacagga catgacatcg tttctccacc atcacattct gcttttacaa
    57961 cacctttgga attaatggat tttgtgaaat tattgcgtga aaaatcaggt ggaaaaccaa
    58021 taggaatgaa aatttgcatt ggtaacaaat ctgaattttt gtctatttgc aaagcgatgg
    58081 ttcaaacaaa aacctacctc gattttgtaa cggttgatgg tggtgaaggt ggaactggtg
    58141 ctgcgccaca agaatattca gaccatgttg gaatgccgtt gcgagatgct ttggcatttg
    58201 tttatgatgc tttaaatggc tttggaatta aagaccaaat caaaatcatt gcttccggaa
    58261 aagtcgtaac aggatttgat attataagaa acctatcatt aggtgctgat ttatgtaatt
    58321 cggctcgtgg aatgatgttt gctttgggtt gcattcaagc attagaatgt cacttaaata
    58381 cttgccctac tggtgttgca acacaaaacc ccaatttaat gaaaggatta gttcccgaag
    58441 ataaaagcgt tagagtaaca cggtttcagc aggagactgt aaaaagtgcc atcgatttaa
    58501 tggcttcagc tggaatacta catccagatt atgtgacacg cgatgtcata accactcgaa
    58561 ttgacagaaa caaagtagaa acttttgcac aaacatttcc agaactagaa accggatgtc
    58621 tgttaaacga aaatacagtt ccagagaaat ttttgtattt ttggaaaaga gcaagtgtag
    58681 agaaatttta aatcaaaaca atgaataatt acttcataaa acaacaaaaa atacgtttca
    58741 aacatgtcga ttatgcaggc atcgttttct atccacgatt tttagaaatg ctaaacgatt
    58801 tagttgaaga ctggtttgaa gaagcattaa acagaccttt ttcaaaaatg cacgatgcta
    58861 ctaatggtga aagctacggc attccaactg ttgatttgaa agtccaattc aaaactccag
    58921 ctcgaatagg cgaagtgata actaaaaaac tatgggtaac ccaattgaaa acctcttctc
    58981 tactttgtgg tttccaattt gtaaaccaag atgataaaac cgttctcgaa ggcgaagtaa
    59041 ccttggtaaa cgtaaaaatt gaaaaagaaa aagatgctat aaaatcagaa gcttttaatg
    59101 aagaaatgaa gagtaaaatt tcaactttta tccagtctta atacttaata caacaatctt
    59161 aatactaatt catggacttc aaaaaattca aagccgcaac cgttcaaacg tcacctgttt
    59221 ttcttgatgt agaaaaaacc attgaaaaag cgatttcttt catcaaagaa gctagtcaaa
    59281 atggagctac attaattgct tttccggaag tatttgttgc tggttatcct tactggaatt
    59341 ggattatgac acctgttcaa ggaagcaaat ggtatgaaaa actatacaaa tgttccttaa
    59401 aaacagacga tgaatccatg aaaccgttgt ttcaagctgc aaaagatttc aatatacata
    59461 ttgtcattgg aatcaacgaa cgaggtaatt cttatggtga aatttacaat actaatttaa
    59521 ttatagataa taacggaaaa ctaatcggaa aacacagaaa acttgttcca acttgggcag
    59581 aaaaactaac atggtcaagt ggtgatggtt cttccttaaa agtatatgac acagaagttg
    59641 gaccaatcgg aactttagcc tgtggagaaa atacgaatac tttagcacgt tttacactac
    59701 tttctcaagg agaattagtg catatagcca attacatttc acttcctgtt gcaccacccg
    59761 attacaatat ggcagaagct attaaaattc gtgctgcagc gcattccttt gaaggaaaat
    59821 tatttaccat cgtttcgtgt tcaaccattt cgaaagaaat tatgaatgcc atgaaagatg
    59881 atgttcccga tattgaagaa ttactaacgc gaaaaaactc cgctttttca ggatttattg
    59941 gtccaaatgg tgcagtaatt ggcgaaccat taattgatga tgaaggaata atttatgcca
    60001 atattgattt agaaaaatgc atccaaccca aacaaatgca cgatattttg gggcattaca
    60061 ataggtttga tatttttgat ttaagagtaa atacggctcc aacaagaaag attactttta
    60121 tcgataatca tgaagagttt aacaagaaat aaaaaaaatt taaaatcgtt ataatattat
    60181 tgtttatgct ttttagcaaa ttaattttct ctagtcaaac ttatgagttt gtatcatgag
    60241 aacctgcgga actattcttt tataactata caaagttaaa acgaaatgca ataattaaat
    60301 atataaattt taaaaatctc aagagataat ttgtatttcg gtattactag attttattgg
    60361 ttttggattg tttttgtttc atggtaaatc attgcggtat atataaaaaa aaacacccat
    60421 tatattttga agaataatgg gtgtttttga tttgtaacat tatttttaat ttataattag
    60481 tttcttagtc accgtgtttt tgtctttttg gattttcaca aaataaacac cagtactcaa
    60541 ctgaattgaa ttgaaattaa cagcatcatt atctgccaat tgttgcacta caacatttcc
    60601 taaaatgtct ataacggtta tggtagcacc atttaactct tttcctgcta cagaaaagtt
    60661 cccgtaggaa ggatttggat atacttttat atcattatca gtagctaaaa cagtatttga
    60721 aagcaatgaa ttgacagaga aactataatc aaatcctcca ccaaaatcgg ggttgaaagt
    60781 ttttataatg acgtcgtttg catctaaaag tctcatatag cctgtgcctt gtgaagtgtt
    60841 tgcccaccat tgcaaaccgt ctttcccaga atcgtttact cttaatctgt aacatccatc
    60901 accaatttgt ggttcttggt aggtataggt gtaaactgta ttagctgttg gaaacgtttt
    60961 agtttcaatt agtgttccgt ttgaatcata taatgagtaa ttgttttgtg ttggtgaatt
    61021 gttggttttt aattgaattt taaaaacggt tggcatcaca tctggaagag taaaactgga
    61081 aattagatga ttgttattgg aatacatatc ggcaccgtta ttgaccgtca tgatttgagc
    61141 atgaaattta ttgtttgccg cttctacacc attattccaa agtgcatttg ttggtaaaaa
    61201 aacatctact gaagccattg atgccaaagt tcctgtccaa gtataagttt gatgtgtact
    61261 tgcagaattt aaccaatatt ctatttcaac attagtcaaa acagtctcgc cggtattgct
    61321 taacgtaatt ttaggacgct cgcacattgg gtttattctt gagaattccg catttttatt
    61381 gttaggcatt ttaatttcgg tgatggcagc atctaacgca aagtttgcag gaccatatcc
    61441 tactacttga tgtgcaacaa tatatctata gtctcctgtt ggcacttgag gatttgatgt
    61501 tctgtaatca atagccattg ttgtgcctgg agttacatgt ggagttaaat tttgttcttt
    61561 gagtaatgtt ctttgtccag gacaccagcc ttgtctgttg taaacccaag ttcctccttg
    61621 aggaaatata gggttttctg aacagtcttg agttattgac catgaaaatt tttgcacgtt
    61681 atttaccaaa attttatggc taactgttcc tccattttga gaaaattcac cttgagaacc
    61741 atgacctgta atggatgatt ttaatttgaa cgctgttgct tgtgcgctaa agtcataatt
    61801 tacggttggc attttagtcc cgttaatgat ttcattaata ttaggactac ctaatctgta
    61861 agatccttgc cataattggt cgtattgaac aacgtttctt ggtggtgttc ctacaataaa
    61921 ccaaaattct aggtctaagt cttcttgcca aacgccacca ttcattacaa ttcttttatt
    61981 gccttttaaa attttcacat aatcggacat gtcaaaatac caagattttc catttggtcc
    62041 taagtctaaa ccaattccgt aaggtgttac aaaagatact aattcattgt agaacgggaa
    62101 tcttttgtaa taatctaagt ccactgcgtt tattgtgcct tctacaggaa ctggaatggt
    62161 agcaattatg tttccagtat ctccatcata aacattttct ggagttgcct caaataagtt
    62221 gaccgaagac aatagattta tcgcatcatg ttgcattgga gttactcctt cgtttgaggt
    62281 tattgaataa tggttgacca cattagcatt ttttggtgaa gaatatctaa caataacatc
    62341 agcaactgtt tggtcatagt ttcctctata aagtgtaatg gtaggcacag ttgatgattc
    62401 aataaaagtt gttgaaagtt ggtcgcctct ttcataatcc caattcaaat taattccgtt
    62461 tgaagtaagt ccatttttgg aatcatttac cacttgacct gttagttcgt ctagtttgta
    62521 ataggctagt aaattagaat aatctggatg ggtagcatca atggttctgt ttttccagtt
    62581 aagtattgat gtagcagaaa gcgctttgtt ccaaattgta aactctctaa ttttaccttt
    62641 ataacctgct aaagttccac catcatcaaa ttttcctaaa accaaattga gaatagtaat
    62701 tacattcgtt ttgctagtac ccgtgtgcca taaaactcca tttaaataga tgttcatcac
    62761 gccagttgtt gcgttttttg taaatgccca atgattccaa actcctttga taacgttatc
    62821 agttgcgtat ggtttttcaa ttctatcata ggaaccatta acattaccac aatcaaaata
    62881 aacacttgaa ccccaaggaa tgtgtaaatt aagttgacgt tgtgaaatgt tttcgccaaa
    62941 agcatacaca atactattgt taacaggcat aaggttttca tcgccttttg tccaaaaagt
    63001 aacggttact tcgttattaa ttgtattaaa aaattgtgca tttatgtcca caagaccatt
    63061 agatgaaaca tcaattgcag caatattatt ggcggttaat gttcttgttt ctacatttgt
    63121 aaaacctgaa aactcaattg gagttgaagt tgttgtgttt gaaaatgaaa gttctacaat
    63181 aatattgctt gttccatccc aattatatgg tgtgtgaaat tgtaatttat ttgttccagc
    63241 tacaaaagta taatcgctaa aaaagacttc tgtaaaacca ttgaagtcaa tattctgagc
    63301 ttgcaattga gtcagatttg tattttttat tttaactttt aaaaattttg taagtccacc
    63361 agcatttgta acatttaaac ttaaagcatt tattaatcca gaagaaaatc cagaatttaa
    63421 aagttcagat gctttaataa gtatttgggt gcgtccgctt tttaaattgg tattcaacac
    63481 atttgaaacc gaagtattat ttgctccaac aacataattg ttttcattaa taatattgtt
    63541 tacaataaca ttcttttgtg taaagtcgta atgattatat gtttgttgtg aagtataatt
    63601 aaacgttgtt cctgcaaaat ttgaaactac ataatcaggt tgtttgaata gtactttctc
    63661 aattttagaa tcatctgcta taaaagtatt gcaagaataa tcccattctc cacaacctaa
    63721 atctctttgt gttcctgttg aaaccaatcc atctttacaa cgcatgttgt atttaaggat
    63781 aattttttcg aaagttaaac cagggtcgtt tggaaaatta attaaagtat ctcgcgtggt
    63841 actattaaaa ttaaaagttc taactttaat ggtatcgcct acactttgcg agtgtattga
    63901 aagcacaggg attattaaaa atgtaaaaag aagtagtttt tttttcatgt tggttgtgtt
    63961 atattatgaa tatcatgcag ttgtacaaaa atactaaata taaaacctaa atgaattaat
    64021 ttacttctct atttctctta gattttccat ttttttgtgt gccaagaatc ctttgatgtc
    64081 ttcaaaatgt tctttgaccc ttttatttcc aaattcgaaa actttggtag ccaatccatc
    64141 aaggaaatct ctatcgtggg aaactaatat tagtgttccg tcaaaatcac gcaaagcatc
    64201 tttgatgata tctttggttt tcatatccaa gtgattcgaa ggttcatcca aaatcaaaag
    64261 attaactggt tcgagtagta attttatcat tgccaaacgg gttttttcac cgccagaaag
    64321 tacttttacc tttttggtta catcgtcacc atgaaacatg aatgcgccaa ggatgttttt
    64381 tatttgagta cgaacatcac caaccgcaat ggaatcgata gtttcaaaga tggtcatgtt
    64441 ttcgtctaac aaggaagctt ggttttgagc aaaatagcca atcatagcat tatgccctac
    64501 ttctacacta ccgccattga tttcgatttc tttcataatg gctttaatca tggtagattt
    64561 tccttcacca ttcttcccta caaaagctac tttatcacca cgttcgataa caatgttggc
    64621 atctttgaag attagtttat cgccataaga tttttctagt tctttaacca ccacaggata
    64681 ttgcccagaa cgaatagacg gtggaaactt taactttaat gctgagttat cgatttcatc
    64741 aatctcaaca ggaacgattt tctccaacat cttcacacgg gattgtactg ataaagtttt
    64801 tgagaacgta cctttaaatc tatcgatgaa ttcttgattt tcggcaatca ttttttgttg
    64861 ttcgtcatag gctttttgct gatgaattct tcgctctttt cgcaactcta aataatgaga
    64921 atatttggct ttgtagtcgt atattcttcc catcgttact tcgatagtac gattggtaat
    64981 gttatccaca aaagctctat catgcgaaat aaccacaact gcttttgccg agttgattaa
    65041 gaaatcctct aaccactgga tactttcgat atccatgtgg ttggtaggct cgtccaataa
    65101 aatcaaatca ggtttttgca aaaggatttt tgccaactca attcgcattc tccaaccacc
    65161 agaaaactca gaggtttgac gtttgaagtc ttcggcagta aatcctaatc cttttaaaat
    65221 cttttctatt tcagcttcgt aatttatttc ttcaatggca taaaacttct cgctaagatc
    65281 agatacacgc tcgataagct tcatgtattc atcactttcg taatcggttc taatggtcaa
    65341 ttgctcattg atatcatcaa tgtcggcttt cattttgaag atttcgctaa acgcttttga
    65401 ggcttcttcc atcactgttg aaccgtcttt agtaagcaaa tgttgcggta aataggcaac
    65461 gactgcatct tttggtgcag aaatatttcc tgttgaaggt tttgcttcgc ctgcaattat
    65521 ttttaataag gttgactttc cagcaccatt tttacccatt agggctattt tgtcattctc
    65581 gttgatggca aaagacacat cgctaaataa agtggttccg ccaaaatgaa cggagatatc
    65641 gtttactgta atcatttttt tagttgggtg ttaagtagat ggaagttgga aattcaatct
    65701 gctaattttt tcaagcggca aagatagatt tttttgtgga tttgaagatt tgttttttaa
    65761 agtgcattac cctaaaaaac aataaatact attccacaat ggcttcttgg atgatttttt
    65821 gaatatcttc tcgaatattt tctttggata gacgattgga cgcgttagaa tcggggaaag
    65881 ttctattgga cagaaagatg tagataatat ctgtttcagg atcagcccat gccatcgttc
    65941 ctgtaaaacc agtatgccca aaactagaca ttgccacaca gccacaagtt ggaccagaag
    66001 taccgagttg cggcttgtca aatcctaaac cacgacggtt tccgctttcg caaaaataac
    66061 aggtattgaa atcgttaaag gttcgttcgg aaaaatattc tttgtcgcca taggttcctt
    66121 tttgaagata catttgcatc atttttgcca aatctaagga gtttgaaaat atacctgcat
    66181 gtccgccaat gccaccttcc attgctgctt ccatatcgtg aacgtatcct tgaagtgtgg
    66241 tatatctata ataggtatca atttcggttg gagcaataag actagcatca aactttcgca
    66301 atggattaaa catcgtattg ttcattccca ttgatttata aaaatttgta taagtgagtt
    66361 cgtctaggct ttttccggtt actttttcta agtatttctt caaaatgatg aacgtaaaat
    66421 cgctgtattt gtattctttt ttatccaaca aaggactatc aatgattttc ttcatgatag
    66481 tatcctcata atcgtttcga ataaaaaggt tttcacatac ttgtttagaa aactctttgc
    66541 tataggtttt tcgatagtat ttctcggatg gttttccgtt agcgtctaag gtttctttgt
    66601 aaaaaggaat ccaagcttgt agttgtgcat aatgagacaa caattctttg aatgttatat
    66661 tctctttgtt gctgttttta aactcacgca acatttcgcc caaagtagtt tcgagattga
    66721 ctttgtgttt gtcataaact tgcataacgt tgggcaaagt agctaccatt ttggttaatg
    66781 aagccacatc gtataaatcg gtattcttaa cttttacttg tttggcatag gtttgatagc
    66841 caaatgattt ttgataaatc acttttcctt ttcgagccac taaaacttga atgcctggtg
    66901 tcattttagc atctattgct ttgttggcaa tcttatctat ttgtgctaat ttagtagcat
    66961 tcattccaac attttcgggt agagtaaaac ctaaacgatt tatactttct gttttaatac
    67021 cacttcctgc tctgaattga ttggttatag aaactggaag ttttcctttg gcacccagcg
    67081 caccaaaaat gatttctgcc gaaacttctt gtgcaatagg attgttttgg taggaagcga
    67141 caacggctgt tatattgtca aaatttggaa tttgcaacat cgaatacggt ttggcaaaca
    67201 catctaaaat aactttgttt tgtttcgata ttccttcaat caattgaatt tcttgcagat
    67261 taaagtcgtg attcttccat gctttatccg atttgtgaaa accaataatt acagttccaa
    67321 aatctttaag ttgtattttg atactatcaa gattggtttc tttgatttcg gttatttcgg
    67381 tatatttttt taaagttgaa acaaaagtgg tgttatcatc atcgcctagc ttaatgtatg
    67441 caatcttttg gtcgagtttt ttaattggaa gtaattcgtc atcatttttg attacagtaa
    67501 ccgcattttc atacaattga taggttaacg catttttggc tggcatattc aactcgttat
    67561 ataagttgat tagctctact ggttcgtatg cattgagtcc tattttgtat ttgtatttta
    67621 aaattttctt taccgattct gccaatcgct cttcggtaat aacaccatct tgataagcag
    67681 ttcttaactt ctcaacagca acaggaacat tttccgaaaa caataaaaca tcatttcctg
    67741 ccaaaaatgc ctcaagatct atatcgcccg gttttttgaa attgcttgct gctttcatgt
    67801 tcaaagcatc tgtaaaaatt aagccatcaa aacctaattc tttttttaat aaatcggtaa
    67861 ccacattttt tgacaatgaa gtaggaacgt ttgcatcgct ttctaaactt ggaacgctta
    67921 aatgtgccac cataactgaa accattcctt catcaaacat tcttttataa ggatataatt
    67981 ctatcttttc aatatgttct ttagaagcag taaccaatgg caaagcataa tgcgaatcgg
    68041 ttgaagtatc accatgacct gggaaatgtt taccagtagt atatactccg tttccttgaa
    68101 ttcctttcat taaagcgatg gctttttcgg aaacattctt tttgttttca ccaaaagaac
    68161 gaaaaccaat aattgggttt ttaggattgg tatttatatc tagaaccgga gaaaaattaa
    68221 agtgaatacc caatcgcttg gattcttttc ccatattttc acctactttt tcgattaatt
    68281 ttaaatcttg aatggcacca agtgtcatgt tccaaggata acgataggta gagtctaaac
    68341 gcatactcaa accccattcg gcatctatgc caataaataa aggcactttg gctttggctt
    68401 ggtagcgatt tgtcaaacga gcttgtcgtc ctggtccgcc ttgaaaaaag attaaccctc
    68461 caattttata atcggaaact aatttatcaa tttcggcact gtgagccgca tctttgttag
    68521 aataagcagc aaccataaag agttgaccca ctttttcttc aaaagccatt tggttgtaaa
    68581 tactatctac ccaaactgtt tctccttcgg tttttggaaa aaaatcacgg cgttcatttt
    68641 taattctagg aatataaact tcttcgtcta ttgttacttc tggaaattca atttcttgat
    68701 tttttggtac agtaggaact tttgtcactt gcttgttgat agaacaattg acaaaaatga
    68761 aaaaaacaac gactattact attctgtaaa ccaatttact cattaaaaaa taggatttta
    68821 aaagaaacga ctgtgccaac tttcttctgc aggaacttcc cagttattgt taaattcttc
    68881 gattgtattt actaaattat taaaaacaat ggtatttgca gtaacagctt tttcttttgc
    68941 cattcgttta aagtcaatca atgatttatg agcaatgtgt tctccatttt taaaggtaac
    69001 attcatttta tctaataaat ctactttgta tttttgctct aaattttgaa taaaagcaac
    69061 agaagaatcg atagaacaac ctgtagcttg ttgtacctct tgattgacag aaaggatgat
    69121 aaaacgattg tattttagta taaaggaagc ctccaaacca gttccgtgag ccgaccaatt
    69181 ttctacaaac gtttttaaat cattttcaat ttctgtgatt tcttcgtcag agaattttcg
    69241 gttggattgg tatatccata tttttgaata ttcgggtaat gtatcgaaag gaacaaacat
    69301 cttttttagt attttaggtt gttggtagta ggtgttaggt cttggatttt aggtttgtaa
    69361 atttctttaa aagatttttt ttaaccattt atcctacgcc ttaaagccaa atacctattt
    69421 ataaatcttg cgcatttgca attaattcgg caatatccat cactttgaca gttgcttctt
    69481 tttctttatt tttcactccg tcggtcatca ttgtgttgca aaaaggacaa cctgctgcta
    69541 tgatgtctgg tttggtttct aaagcgtctt ctgttctttc tacgtttact tctttgtttc
    69601 ctggttctgc atctttaaac atttgagctc caccagcacc acagcacaaa ccattagaac
    69661 gagagcgttt catttctacc aattcggcat ctaatttttg aattaaatct cttggagctt
    69721 cataaatatt attggctctg ccaagataac atgggtcgtg gaaggtaatt cgttttcctt
    69781 taaactgtcc gccttcgatg gttaatcttc ctgtatctag taatgatttt aaaaactcgg
    69841 tgtgatgaac tacttcgtat tttccaccta attctggata ttcatttttt aacgtattaa
    69901 aacaatgcgg acaagccgtt actatttttt tagcttcata agcattcaag acttcgatgt
    69961 tcatcatggc ttgcatttga aacaaaaatt cgtttccagc tctttttgca ggatcaccag
    70021 tacagctttc ttctgttcca agcactgcaa attctacttt ggcacgattt aatattttaa
    70081 caaatgcttt ggtaattttt ttggctctgt catcaaaact accagcacaa cctacccaaa
    70141 ataaaacttc gggttgcttt ccttgtgcta gcatttctgc cattgttggt acgttaatta
    70201 tttctgacat atttagatat tagattttag atattagatt ttagatttca tctaagagct
    70261 aatttatttg ttatttctac tcgtggtgct cgtcgtcgaa tacttgaatg gttacttctt
    70321 tttccactaa atcggtaaat ttacctctgt agcgtgttgc ttttactaag tgattgtcta
    70381 tccaatgata gtttccacca cgtggttttc ccatcaacat tccatggtat ttgaaaccgt
    70441 gttgttttaa ccaacgttct gtatattctc tgtgagtttc tgttcttgac gtgaaaaaga
    70501 aaattatatg accttcatca taccacttat ttaatgttat taaagcatct ggataaacct
    70561 cagctgttaa cattctttct ggttcttcgt tgggaatatc atcacagatg gttccgtcaa
    70621 tgtcaatcaa ataattctta attccttctg gtaaaattgg acttaatagt ttaccgtttt
    70681 cactaattgt ttgcaagttc ttgtcaatgt cttccgtgtt cattttttta ctttttatag
    70741 tttctcctat tcattcaaca aatcctcaag tctcacattt atgagaattt attatttttt
    70801 atttatttaa ttttcgtctt tccagtttaa tctatccatt tggttgtatt gccatggtgc
    70861 tccattgttt tcaatattgg tcatcattgc gtttaatgat tgtggcgcag cactttgctc
    70921 catcactaaa taacgacgca tatccataat aattgataat gggctaatat ttactggaca
    70981 ttcttctaca caagcattac atgatgtaca tgcccaaagt tcttctgttg tgatataatc
    71041 attcaacaat gatttattat caggaatgaa aacaccttta ttagcatcga tattttttcc
    71101 aacttcttcc aatcggtcac gtgtatccat catgattttt cgtggcgaca gtttttttcc
    71161 agttatattg gctggacaaa cggatgtaca tcttccacat tcggtacagg tataagcatt
    71221 tagtaattgt acccagttta aatcttgtac gtctgaagca ccaaatttac ttggaacggc
    71281 attttcatca acaggttgtg ctgcaaatgg atcggcattt ggatccatca ttagttttac
    71341 ttctttggtt acactttcta aattgtcgaa ttgtcctttt ggatttaaat cggcaaaata
    71401 ggtgtttgga aatgctaaaa gaatatgtaa atgtttagag aaatacaaat agtttaggaa
    71461 taccaaaata ccacaaatat gaatccacca agcagttctt tcgattagat gtagttgtga
    71521 aacatcaaag ccaccaaacc aataagcaat atgtgaagaa ataatatttc ctgaattcat
    71581 ttcttgaaaa tgaacatcag tagcattcat gattaagaat agtgacatta acaccatttc
    71641 aaaatacaag atgtaattgg catcgttctt tggccaagtt ttcatttcag aattcatgaa
    71701 acgcttaagt ttgacaacgt ttcttcttat ccaaaaaaca attacggcta ctaaaactaa
    71761 tactgctaat atttcaaacg aaccaattaa gaaaccatag aaacttccca tgaaggagaa
    71821 aactctatgt gttccaaata atccgtcgat gatgatttct aatacttcga tattgataat
    71881 aacaaaacca acgtaaacaa tgatgtgtaa aaaaccagct attggtcttt tgatcatttt
    71941 agattgacca agagcaatca ttgccatgtt tttccaacgt tcggaggaat tatctgaacg
    72001 gttaacatcg cgacctaaat tgatgttacg aactaatttc ttaacattga ttgtaaaata
    72061 gccaattcca gcaataagaa ttaatgcaaa aagtaagttg tcaatatatt ccataatgct
    72121 ttggttttac tttgagatag aatcgtttga aggtgcttga taaggtttgt tctttttacc
    72181 aaacaatgaa acattaatat atcgtgttgg gtttaatcgt aaatcttgaa gcagcaattc
    72241 taattcctta gaagctttgg taaagttgtt gtacatggca tcgtcttttg ccagttttcc
    72301 taaagttcct tttcctgatt gaatatcagc cattaattta tcaaccgatg ctaaggtgtt
    72361 ttctagtttt ttgactgttt caccaatgtt tgctttggct aacgaatcgg acatttttga
    72421 gaaattgttc gaagcatttt ctacattagt cattgtagca ctgatttttg ttttgttgtc
    72481 agccaacatt tcattagcat tttgggaaac ttctttgaat tgggctaatg ttgcatttaa
    72541 attttctaga ctatttttaa ggttttcttt tgtttttgca tccaatactt gattgatgtt
    72601 tatcaacatc gtattggcat tttcaagaac actgtttaac ttgtctttga taggttttat
    72661 ttctttagaa acttcttctg ttaatcctaa tttactagaa gaaaccaagt attcaccaga
    72721 tttggcgatg tcttttgttt ctttatttgg aagaatcgct acttgttttc caccgattgg
    72781 tcctggagaa tacaattctg caatagagtt tttagtaatt ttataatcgc cattaatttg
    72841 aagttcaact accgaggtcc aatcttcatt gagtttgatg ctgcttactt ttccaacggt
    72901 caacccgtta atagttactg gagctgatct taccaatcca tctacattgt catatttgac
    72961 ataaaaagta gtgtaattgg aaagtaaatc ttttcctttt agaaaactgt aaccccaaat
    73021 aaataataaa atggaagcta ttactaatat tgccgtttta atttctctag tgattttcaa
    73081 ggcgctttat ttttttgaca aagttaaact attttataaa aaacttgcta ttattgtttt
    73141 aatgcttcgc caatatcaat tctaacgcca tctcttagcg caataacaaa agctgattca
    73201 aatccagttg ctttggcttg ctgcaataaa actttagcat cggcataact ggttgtttct
    73261 ccatacatgt atttgtaaaa cttatttgtt tcaatcattg agataggact cagtccttta
    73321 aaattacttg gtattagttc taactttgtg ctactagccg agatttgaac tttaaaaaca
    73381 actcctttag ttgctggttt tggtgtttct tttggctttt catctgtttt tggagtagtc
    73441 gttggtgtcg tacttgttga tgcaggtttt gaatcgataa caactggcac tactttgtct
    73501 gatggttttt cgtctgattc gtttccataa gttccgtaat attctttttt ataacgaata
    73561 atggcttttg caattgattc tgctatttcg tcttgtccgt cttcagaatc taatcttgaa
    73621 ccttccgctg ggttagaaat aaaacccatt tcgattaaaa ctcttggcat gtaggctttg
    73681 tgcaatacca taaatggtgc ttgttttaca ccacgacttt ttttgccaac attaataaac
    73741 tctttctgaa tacttcctgc taattcaata ctattattaa ggtattcttc gttttcaact
    73801 atcaggccta acattgattc aggcgatttt ggatcaaagc ctttgtattt ttgttggtaa
    73861 tctttctcta aagtaataac ctggttttct tttttggccg cttcaagatt ggatgcgttt
    73921 ttagataaac ccattacata ggtttcacat cctgatgctt ctctattttt attggcatta
    73981 caatgaatgg acacaaaaat attggcatca gcacggttgg caatgtttgc acgttcaatt
    74041 aaatcgacaa aaacatcagt tttacgggta taaatcacgt ctatatccga atttttttct
    74101 aatagtttcc caacttttaa gaccaccgct aaagctatct ttttttcaat tcttccatcg
    74161 taaacagcac caaaatcatg atcgccatga ccagcatcga gtgtaacttt aaatttttga
    74221 ttgctttgcg aaaacataaa attggtaacc aataaaaaga ttgttgctac tattcctata
    74281 tttagtttct gttcctttat cattattctg ttctttattt tcatttaaaa cgacactata
    74341 aatgcatctg taaaaccttt atcttttgct tcgtcacaca atttcctggc atcatcttca
    74401 tttgatgctg agccaaaata atacttgtat aatttatttt cataaatata ggttatcttt
    74461 ctcaagcctt taaaatcgga tgaccttgta tctctttttc tagaagtaac aaataattgt
    74521 actttatatt tcccactact atttttatct tctttatctt ttgatgatga tttggaagaa
    74581 tcaatttcaa ttttgtcttc tataatttct tttctagcac tctttttagt atcgttggcg
    74641 agtaccaatt cgttaaaatt ttctctttta tatgaaatga tggcatctgc tatttgtcgt
    74701 gccattttgg tttgtccttc gtcagaattt aaaaattcac cttcttcttt gttggacaga
    74761 aaacccaatt cgattaacac acctggcata taggcagcat ccagtaccca gagtggttgt
    74821 tgttttacac cacgggtttt tctattcaaa ttggaggtga aattatcttg tattccagaa
    74881 gccaagttga tactgttgtt taaattttcc tcttgaataa tctttaaccc aagaaaagtt
    74941 tctggtttgt gtggatcaaa accttcataa gtctttttat aattatcttc caaaaagatt
    75001 actgagtttt cattttttgc cacttccatg ttcatatcgg ttcgtgataa acccataaca
    75061 aaggtttcgg ttcctgcagg ttcaaagttt tttaccgaat tgcaatggat agaaacaaat
    75121 agattcgcat ttattttgtt tgccactttt ggacggttaa ccaattctat aaaaacatct
    75181 gattttctag tataaactac ttcaaaatct ttttcatctt ttaaaaaatc gcctactttc
    75241 aaagttgttt ttagcgcaat atccttttca acatagccat gataggaatt tccaggatct
    75301 tttcctccgt gtccggcatc taaaaccact acaaatttac ccgattttga ctgcgcaaac
    75361 gctgtcaaaa ggttaaatat tagtagaagt gaaatcgcta ttttaatctt ttttggaaaa
    75421 aacattatgt ataaaagtta atttaaatta aaacctaaag gttataaagt taatatttgc
    75481 ttatttttga gcgcaaatta ttagtaagtt tgctgcttca aaaaccgagc catagatata
    75541 caaaaatagt attaaaccct tgcaaagaaa cttattttat atcgtttttt taacaatctt
    75601 cctaacatta ggaaaaggga aaagctattc gcaagaaata cctaaaggct ccacttcttt
    75661 tcctgctaaa aatcaaacaa ataaagaaga aacggctccc gttattgaag aaaacttggt
    75721 ggttactgat accgtaaaaa tggattctat aatcaaaaag aaaccgatta tagaaagtaa
    75781 aattagaaga aaagcggttg attatgaaaa aataaatcaa aagaaaaaac aaattacgct
    75841 ctataatcaa gctgaagtct attatcaaga catcgagtta aaatcgggaa taattgttat
    75901 tgattatcaa aaaaatgaag tttatgccgg gaggattaag gattctacag gtaactatat
    75961 tcaacatcct gttttcaaac aaggtgctaa cgttattgaa cccgattcta tccgatttaa
    76021 ttttaaaact aaaaaagcaa aggtttggaa ttcgagaact gaacaaggtg aatttagagt
    76081 aaaagccgca gaaacaaagc gcgaaaacga ctctgtttat tatatgaaag gtgctcgttt
    76141 tactaccgca aaagacattg atgaccctga atattatttt caaacgaaca aagtaaaatt
    76201 tgttccagga aaaaaaattg tagtaggctt gaccaacatg gttattgccg atgtgccaac
    76261 gccaattgct ttaccttttg ccttttttcc gatgaccgaa accagcaaat cgggaatcat
    76321 catgccaacg tataacgata gtaatctcag aggattttca ttgcaaaatg gtggttacta
    76381 ttttgctcta agtgatagtt atgatttgtc tgctcttggt gattattaca ccaacggtag
    76441 ttatggaatg cgtcttcaaa gcaattatgc caaacggtat aaatttaatg gaaacttaaa
    76501 tcttcgttac gaaaacctag tcagtagcga acgtggtttt cctgattatt cgcgaactac
    76561 tatttacaat attcaatggt cgcatgccca agatgctaaa tcaaatccga gttctcgctt
    76621 ttcggcatct gtaaatttgg gaagtagcca atattttagg cagtcggtaa accaaattaa
    76681 caatggttca caattaaaca atactttgag ttcatctatt tcttattcca agacttttaa
    76741 ttcggtgcca caagtcaatc tttctttaac agctacccac caacaaaaca ctaatacgca
    76801 agagataaat atgacattgc ctacattgca agctagtgtt gatagggttt tcccttttgc
    76861 accaaaagaa ggtatcaaaa aaggttttat caaaaacatc aattttcagt ataacttaag
    76921 aggagaaaac agaattcaca cttatgattc gttgtttttt aaacccgaaa tgtttcgcga
    76981 tgctaaatta ggctttcaac ataccattcc gttgagcacc aactttaaac tatttaagta
    77041 ttttagtgcc acttcatcgg taaattataa tgaggtttgg tattttaaga ccattcaaaa
    77101 agaatatgta gcttctcaaa atactgtagt tgatactgaa attccaggtt ttgatgcttt
    77161 ccgcacttat tcgttttcaa ctggagttgg aaccactatt tatggaacat ttaattttaa
    77221 aaaaggtaaa aaattacaag ccattcgcca tgtgatgcgt cctaacgtga cttatagcta
    77281 tacgcctagc ttttctaact attatgacac ttatgatcct gatggaagtg gtactattcg
    77341 aaaagactac actcgttttg atggaggcat ctttggagca cctggaaaaa acatgtcgaa
    77401 cattatgagt tttagtctta acaatacaat tgaagctaaa gtaaccgaca aagattcgac
    77461 caaaacagag gctagaaaaa tcatgttgct aaataattta aacttttcga caagttatga
    77521 tctttctgcc gattcgttaa gattagcacc tatgcgaatt agtgcaggaa cccaactgtt
    77581 taaacagaag cttgacatca attttgccac aactcttgac ccatatgcca tcaacaatac
    77641 tggtaggaga attgatactt ggaatattga caacggagga agtttgttcc gaatgacgag
    77701 ttctaatatc actatgaact atcgtttttc gagtaccgat ttagacgaaa aagaaaaaaa
    77761 caaagctgga aaacaaaatg gtggacgtga agacaatctt tttggaaaaa acacagactt
    77821 aagcgaccga cgggaaagcc aatttggcga ggaggacaaa accgaagacg aaacacccac
    77881 cgagtttttt aaatacaagt taccctttga cctaactttt gcctattcgc ttacgtattc
    77941 taatattaat agggaaaaca aaatcactgg taactccttg atggtttcta tgaataccga
    78001 cctaacgcca aaatggaaaa ttggggttgc tacgggttac gactttgtgc aacaaggcgt
    78061 tacttttact caactgcgct ttgaacgcga tttattgagt tggcgcatgg attttaattg
    78121 gtcgcctttt ggtgaaaatg ctaattgggg tttctttatc ggaattaaat cgggtgtgct
    78181 tagcgatatt aagtgggata agcaacgatt gccggataga gttttgagat aaatttagag
    78241 tttagaagtt agaattatag aatacttatt tatgaaaaaa attatattta ctgctaaagc
    78301 acctgctcct attggacctt acaatcaagc ggttttaatt aaagacacgc tttatacttc
    78361 tggacaaatt gccttgcatc ctgaaacaat ggaattgata ttggacgaca ttgaaacgga
    78421 aactaagcag gtaatggaaa acatgaaagc ggtgcttgaa gctgccgaaa tgacttttga
    78481 gaacgttgta aaagcaacta tttttatcat gaacatgggt gattttgcta gaataaacag
    78541 tgtttatgga agttattttg atgaaaaaac tgctcctgcc cgagaaactg tgcaagtggc
    78601 atgcttaccc aaaaatgtta acgtagaaat ctctatgatt gctgtgaaat aaacctaatt
    78661 agtagccacg caatttattg cgtctccata aaacattaaa gaacctaata ctcacaaaag
    78721 attattaggt tttttttatg tggtttttgg tggtttgttg atatggttat tggggttggt
    78781 ggtacggatt atatatccgt aatatttacg agccaagtac ggttgaactc attgataaaa
    78841 tcataaccgc ccgatgcatc ccattttcct ttgttgactg aaaaaattta cagttttgaa
    78901 tgggaaagcc gttgtttgat ttatttttta ttctaaactt tattttgata ttttcataat
    78961 acattgtaat atacataatt aaagaaatgt ttataagtta ttgaaaatga tcatattata
    79021 atttgtataa ttaaaatatt gatcaaatat ttgcatcata ataaattatt atgaacacat
    79081 cttttgaacg tagtgaaaac acaaaagtag aatggttaac tcctccagaa ttagtaaaat
    79141 gtctaggagt atttgattta gatccatgca caccaattaa tacccctttt ttccatgcca
    79201 aaaataatta tactactttg gatgatggat taaagaaaga atggttcgga agagtatatt
    79261 taaatccgcc atatggtcga ggcatggaac tgtggttaga aaaattaaag tttcatggaa
    79321 atggaattgc attaattttt gcgagaacag aaaccaaatg tttttttgaa catatttgga
    79381 atgatgctga tgcggttttg tttgttaaag gtcggattag attttaccat gtttctggtg
    79441 ttcaaggtgg tacaccaggt tcacctagtg tatttattgc ttatggtaaa gagaatgcta
    79501 atactttaaa aacttgtggt attgaaggtc gatttttaaa tttaaaataa tcccgtaggg
    79561 atgatatgct agattggttc tttaaaaata taaagctcat tgtattcgat ttcaaatgct
    79621 ttcagaaaat ctatatattc ttcttgaaat gttttggttt tatggtgttt ttcttggtta
    79681 gcaatgtatt ttgtaactgc atctacttgt gattttgaat aagagaaagc gccatatcct
    79741 tcttgccatt caaattttac ttttagaaac tttttttcgt ttatccattt ttgatgagtt
    79801 gccttttata tcttgtagtt atatcatccc tacgggattt tttattgttg ttatctttat
    79861 tttccgtggg ttgaaaccca cggctacaaa catataacat ccctacggga ttttgatgtt
    79921 tatagtattt catagcaaat gaattcggca atattatatt cagcatcaat tatccattcg
    79981 ttttctatca atagttttat taaagcttga tcttcattat ctacgttttc aactatgcca
    80041 attacttgca tattgtttac ttcgtatgta ctatttggag cttccgttgt tccttcggga
    80101 gaaataaata tatatttttt catttccata attttctgta aactccattt cctaaaaatt
    80161 ctaataatcc aatatctctt agattctgaa gttgttgcct tatttttggt gcaattcttg
    80221 tatttgttac gtgtttttgt tttagaactt cctcaaaatt ataaacatct gctaatgtaa
    80281 agatttcttt ttcaatggaa tctatacaat tgaaaacatc aagcttccaa ccatttaatg
    80341 attgtgttat actatttctt gataattgaa ttacatcgtt tttatcctct tgattttcaa
    80401 tttcaaattt tggaaattta gtagtttcgc ttttagcaat attagaaagt attttattta
    80461 cctcagctat atgtctgggt tctatagtac ccgttttttc gtttttggag attaaatcaa
    80521 aatcagttac tacttgggaa actattgctt tgtcatttgt ataaacgcca tattcaaaat
    80581 tatttattaa tcctccattt gtcaaatttc cggagctaat aataacttct ttatcatcaa
    80641 aaagatagat tttggaatgc aattttgaat ggtttttaac aattccatct ttatttataa
    80701 tattttcaat cgctttaata tctaatgaac ctgaatgaat attacctaat ttaaaagatg
    80761 ttattaattc taacttaaca ttggaagatt ttgcgattaa catttcatta caaacatttt
    80821 cctttacaaa tggagatgtt atttttatgg aatgtttgga gtttgaaaca agttccagaa
    80881 aattattttt ccaaggtgta gttataattt tcatctgatt aaaatttcat ttgtcactac
    80941 ttttttgccc attttaaaaa aggtttggct tgcatgaatg aatgttgatt tgtgctaaaa
    81001 taataattat gcatttattg aaggctattg tataattatt gttttcaaca aatgtattaa
    81061 atggtgtaga agttaaataa tagattgttt tgtgggatta ggtttttttc gttgttaaat
    81121 agggtctcca cgaggtcaaa gatgccttcc gcgacgattg aaatgggttt cgcaattaga
    81181 tttttttata aatcatccaa ccctaataat aagagttgtg ctgtggcttc attggttgcc
    81241 aatggaatat tgtgtacatc gcaaatacga atgagcatcg atatatcggg ttcgtgagga
    81301 tggcttgcgt ttgggtcttt aaaaaacaat accattttgc attttccttc ggctactctt
    81361 gctgcaattt gggcatcgcc tccttgtggt ccagaaagca ttttttttac tttgatacct
    81421 accgcttcta ctttactacc tgtagttccg gtggctatga gcttgatgtt ttctttttcg
    81481 agcaagtatt tgtttttatt gataaactga accatttcgg ctttctttcc gtcgtgtgca
    81541 ataatggcta tttccatagt gttttttttt gtaaaaataa aaaaaccact ttaaaaaaag
    81601 tggctttgta tattatttat ttaaggcatg gtatgcaatt aacccatcta ttggtcttcg
    81661 caacacatta cccattttaa tattgtattt gtttaaaact atttccaatt cgtcttttaa
    81721 ataaaaagca atagagccta caaaatgaac tggcacttct tgacagtttt caaattgcat
    81781 gatatagttt tttgcaaaag attttaattc ttttttgatt atttttttac aaaactcatt
    81841 ctctttgtgc ttgatgatga attttgcaaa tgttgccaaa taggcattcg gatttggctc
    81901 tttgtataga ttggctttta tgtaatcggc ttctacattg tattcttctt caaattcaag
    81961 agctaactct ttgggcattt tattgaaata atagcctctt agtaaatgtc ttccaaatct
    82021 atttcccgaa ccgtcatcca tagcaatgta gcctaatgat tgtacttttt gaaacaacat
    82081 gcttccgtca aaataactgc aattggaacc tgtacctaat atacaaacta ttgctttttc
    82141 gtcttttggt gtggttgcaa aaacagcagc ataggtatct tcataaactg ctactttggc
    82201 attgggaaaa tactcttcaa aaatagatgt taaaaaaatt ttcattctat cggtaccaca
    82261 acctgcgcca taaaaacaca aactagtcgc tttgttttta ttttgtgcta tatcaaactt
    82321 atcttcaaga cgttcgatta cttcttcttt ttcaagtact tctggattca aacctaaggt
    82381 ttgagtagta aaaagcactt tgccattatc gtcaatggct atccaatcgg ctttggttga
    82441 accgctatct acaagtagtt tcatagttaa ttatgagtta taagttttga catatgagtt
    82501 ttattaactc ataaaaacag caatcccaac tttgggttgc aaaatcagga ttgaaattta
    82561 attatatttt tgaaatgtga acggctaagt ctattaattt acttgaatat ccatactcgt
    82621 tatcatacca agaaactatc ttgaagaatg ttgagtttaa cccaattcct gcttttgcat
    82681 caacaataga tgttctttta tctgaaataa aatcttgaga aactacttga tcttcggtat
    82741 atcctaaaat acctttcata gaagtttcgg aagttttttt gattactgcc atgatttctt
    82801 cataggtagt ttctttggca acttttactg ttaaatcaac tacagaaaca tcgacagtag
    82861 gaacacggaa tgccattcct gttagttttc cgtttaattc tgggattact tttccaactg
    82921 cttttgctgc acctgtagaa gatggaataa tgtttacact tgcagcacgt ccgcctctcc
    82981 agtcttttcg agaaggtcca tctgcagtca tttgtgtaga agttgttgca tgcaccgttg
    83041 tcattaacgc ttcaacaatt ccgaaattat catgaataac tttagccaat ggtgctaaac
    83101 agtttgtagt acaagaagcg tttgacacta ctaaatctgt agctttaact gattcgtggt
    83161 ttactcccat tacaaacatt ggagcatcag ccgaaggagc cgatataatt acttttttgg
    83221 caccaccttc aatgtgagca cttgctgttt ctaccgttgt gaagaaacct gtacattctg
    83281 caacgatgtc aacatcaact tctttgtcat cccattggat taattttgga tctttttgag
    83341 ctgttaccct gatgaattta tcgtttacat aaagttgtcc gtcttttact tctactttac
    83401 cattaaatct tccgtgaaca gagtcatatt ttaataaata agctaaatga tctacatcca
    83461 ataaatcatt gatggctact acttctacat tatctctatt cattgattct ctgaaaacta
    83521 ttcttcctat tcttccaaaa ccgtttattc ctaattttac ttttgacatt ttttttattt
    83581 tatattaatt tggcaatttg ataatttggt tattcttgat ttcgattaac cgaatcctca
    83641 aataactgta ttttgtttac gttgacatga tgtccgacac tcttaataat tctctattta
    83701 tttcggtgtg tcctttgata gcttgttcta atggtgttaa ttcgatttcg tctttaannn
    83761 nnnnnnngta tcgattctac tgcttttact cctaatcgtg aagccaaaac tcggtcaaaa
    83821 cacgatggtg ctccaccacg ttgcatgtga ccaagaactg atactcgcac gtcatattcg
    83881 ggcatgtgct cttcgacata atcttttagc tcaaacacat tttttcctaa tttatcacct
    83941 tcagaaacca caacaatact tgaagatttt ccggaagctt tacttttttg tagtgattct
    84001 aatagtcgtt ctaatcctaa atcttcttcg gggattaaaa tctcctctgc tcctgctcca
    84061 attccagcgt ttagcgcaat atgacctgca tctcttccca taacttctac aaagaaaagt
    84121 cggttgtgag aactggcagt atcacgaatt ttatcgataa cttctacaac agtatttaat
    84181 gcagtatcgt agccaagtgt atgactagtg ccaaaaatat cgttgtcaat ggtacctgga
    84241 attcccatta cagggaaatc aaattcttta ttaaatactt cggcacctgt aaacgaacca
    84301 tctccaccaa ttacaactaa agcatctaca ccagctttaa ccaaatgttg gtgtgctttt
    84361 tgtcttcctt caacggttgt gaattctttg gaacgtgccg attttagaat ggttccacct
    84421 ttgttaacga tgttgtttac acttcgtggt cccatttctt taaaatctcc ttcaatcatt
    84481 ccttggtaac cacggtaaat accaacacac tcaatgttat gataagcaca tgttctaaca
    84541 actgaacgaa tagcagcatt cattcctgga gaatctccac cagaagtaag aacgcctatc
    84601 ttttttattg tttttgacat ttttttatat attaaaatgt aaaaataaca aagacaaagc
    84661 agttttgcac ctaatttatc gcggtattaa caaacgaaag aaacttcaaa cgttttcgtt
    84721 gataagtttt aaaaaaatgt atctaaaatt ataaaattaa taatcttctg gaggtaatcc
    84781 ttgattattt gggtgtggtt tatcctcttt ttttggatgt tttgggtcat ttgtaaattg
    84841 ataatgatca ggaagtacag aatcgtctcc aacttcggat ttagatggga ttatttgcag
    84901 taatttttta ttattaaaaa ttttggtgat taattcccta aaagtatcaa aatctacttc
    84961 ataggaaagt cccaatcctt gggtatatcc aacaccttga ccgatataag taacgtcatt
    85021 ttcgcggtta aaaaccctta aattgagtgt accatcatca ttaactctgt attgtagttc
    85081 tacatttcca acaacagctg attcgtttac tccaccgaca ggaactccta cttttccatt
    85141 cactgttatt ctttcattta ttttggaaga aaggctcaat ccaaacctgg catcggtttc
    85201 atatcctggt cttgtgtcgc caccaacata ttctggtgca atggagactt ttccgccatc
    85261 tgtagaaaaa atatctgaaa atacactact ggctttttcg tataataagt tagaagattg
    85321 tgattgtgta acaccctctg ggcttaaaaa agaattggtt gataacaagg ataatgcctg
    85381 agtttgtctt gtgtctttat cgtctaattt atattgtaat tctgaacgta gtgttgaagc
    85441 tactttggga aactcaatag aaaactcagg ttcaggattc attaaacttc ctttaatacc
    85501 aataacaact tcaacatcaa cttttttatt aaatgaagga ttggcaatta gcatagctgg
    85561 attggcggta gttttatata ctgcatcaat attcaatgtt gccgccatag gatcacctga
    85621 ccatacaatg gaactgtatt tttttacttc aaaagttttg ccaaacaaac ctccgtattt
    85681 gaaattataa tatcctttgt aaacctgaaa gtcaccatac atattaaatt tccctaaagt
    85741 gtttatttca aaaagtaatg ttcctctacc atttcctttc attccatgac ctgaatctct
    85801 gttaagaatt acttcaatat ctgcattttc aagaatgtca aaattaaagt ctaattctaa
    85861 accattaaat tttttttctt ctttacttat tgaattatta cttattggtt ttttaaatct
    85921 tatataccca ttttcttcgg ctaattcact atcattgatt ggaattttaa tacttgttcc
    85981 atttactgat tcagcattta ctttaattgc caatccttcg gtaggaccac taattgaagc
    86041 agaaccattc atgaaagcag ttccataata agcagcgtct tccgaatcaa ctgtgtttaa
    86101 ggctagtaat tttttagaat ttatatttaa atctaattcc caatctccga attgtttgtg
    86161 tttgataaat ccattgaatt gtccttcagt atcaaattta gaatctttaa tctcagtttg
    86221 tcttattatg aatttatttt cggtaacatc aacaattgaa ttgttagcaa aagaataatc
    86281 aactcccaaa tatggaattg tcaatcctgt atcattgaca aacaatctac cattataatc
    86341 caattgattg atgtcgccat caattctagc atttccagag gcaaatcctc gaatattcgt
    86401 tatgacatcg ccacctattt tgcctaatac tcctagattg aatttatcaa aacgcaagtc
    86461 aacatctaat gatgtttttc catcataaac ttctatagtt ccatctgctt caaaagattc
    86521 tacatttttg ttaattaagt tagaccttac ataaaatttc tttaaactct catcaccttt
    86581 tatatctaaa tttagttttc ctaatgcaat gtcatttagc attaaatcat caatttcaag
    86641 cgacgatgtt ggttgatata cttgaagatt ttgtttaaaa tagatatcac catttacttt
    86701 tccatcaaat ttaaactttt caacatctgg cgttatcttg ttcaaattca cattcttaaa
    86761 agtaagtttc aaatctttat ttgtagtgcc ttttaaaact ccattcaaat taaccgattg
    86821 gttttcatgg gaaattttga tgttatcaaa gttaaaattt tgcagttttt tatcaaatac
    86881 aattttgttt tggttgtcat cttcctcgtt tacaaaccac aagtaatctt taaattgaac
    86941 ttctgatttt tgcaatccaa caacgttttg gttctctgca ttgatagtat ggtataaatt
    87001 caaattataa aaatcgtttc ctttatcgcc acctttaaac tccgttctaa aatataatgt
    87061 gtcgttctga atattattaa tcatactaaa atctctgact ttatagtatt ttgtatttat
    87121 agaatctaac tgaacatagg acttaaacaa tggattttta ttatcaattt gcaacaataa
    87181 gttatcaaaa gtattttctt tataagtaat tttgggtgat tttaaatcca atttaaagtt
    87241 atccgattca gaactaatgc ttccttttat cttagtgttt tttgaaattg aaatatcagg
    87301 atagaaaatc tcgatgattt tactataaat atcaaaatca aactttatgt attgtccttt
    87361 ttttacttta ttaggtttgt aattggcata caatgttcca atcgaatttt caatcatgct
    87421 tttgagttgg tcaaacttat attttccaac gatagttcct gataatgcat ctgtggaatt
    87481 cacagttatt tttcgttcat tctcttcatc aaaactagag ttaatagaaa ggttgtcaat
    87541 aaaataaata tcttttctgt tttgatacat tgcatttgtt aaaaccacat cgcctttgaa
    87601 attgtctaaa tcatttcctt tagaattaat ggcaatatct cctttaaaaa cagctattga
    87661 atctttaatg atatttaatt tatctagatt tgcatagtca attttagcat tgaaattgat
    87721 tattttttca tttttaccta aatcaattaa accattaaaa tccatgtata agttcgggtc
    87781 atttacaaaa aactttcctt taaaaacagg ttgtttaaaa cttccatcaa caacaatttt
    87841 ttggtaattg taacctctat aattcagttt atatacatct cctacaaaag ttgtatttag
    87901 tagtttttgt gtgaatcctt ttccatcaac atcaatattt aaacttactt tcccaatgtc
    87961 cttttgtcca attaaatctc cgatttcaaa ttggtcaaaa ataacatttc ccttataatt
    88021 tgcattgtca atattatcta aattattaat agtcaaggtt gactctataa ttcccaaagc
    88081 tgtattcatc acaaaatcgg catcaatata cttttgagta acttcggctg ttcccgtaaa
    88141 gttaaattgt cctaattttt ctaaagatgt tggcaatttt ttccctaaaa catttggcaa
    88201 taattcagtt aaatctttat aattagacgt aatcttttta aaatctcctt tcataaaaaa
    88261 ttcacccgga ctacgaggaa aaaggttttt aaaatttaca tcgcctttaa ttatagagtt
    88321 attattttcc tttaaataaa ggtttgtagc ataaaaatca ttcaaagttc cttttacttt
    88381 tgaggtaagc ataaagactt tgtttttacc catttcacta taaaaataac gaatgtcgtt
    88441 tgttgcaatt tttgaattct ttgtttcaat atcaaaaatc actttgttat taaaattact
    88501 aaaatcctta ttgtctttat tataatttaa aattactttt ccttcaacta aggagttttc
    88561 agtttctaat tttaagtttt caagtttaat attccgtttc gtgtaagcaa aatcagcaat
    88621 taaatctttt acaaataatc ctcgatggtc taaaaaagac atttcagcta tttttgccgt
    88681 tacatctgga cctttgattt gaaaattttc taaatgtgca ttaagttttg tgaaatcaac
    88741 atcttttgga acttctcgat tgtaatccat cattacaaag cgtgattttt ttacatatac
    88801 gtttttggat gtcatcaaaa aatttccaga accaggtttt ccgtcgtcta aagcttctac
    88861 gaaaagatcg agattggtgt ctttttcatt tttataattg acgatgttca aatataaatt
    88921 atcaaatctc aaatcgccaa agcgaagttt actattggca atatttttaa aatctaaaag
    88981 actagtattt aatctattta gaaaaataag cgtgtcttga tgatgatcta atatcaaaac
    89041 cgttttcaac ttgacaccgc caaaaaaagt aatagcaact ttgtcaatag aaatatttac
    89101 accatattct ttatttatat catcagtaac atattttgcg atttttgttt gaacaaaagg
    89161 caatgttagt gcaatagccg acagcaacaa aaacactaaa agcgcaatta ttgtccgaat
    89221 gattactttt cggatttttt tgaattgact atttttttta ttttcttcca aatataaaat
    89281 cggcttggga aagactgtaa actatacaaa aatcactatt tttgacaatt cacaacggtt
    89341 tgtcaaatat aaatcaaaat ttgtgccttt ttatgattca agatgaagtt tttatcctag
    89401 ccatcgaaag ttcttgcgac gatactgccg cagcggtttt gaaaaacaat aaagttcttt
    89461 caaatatagt tgcacgacaa agtattcatg aagaatttgg tggtgttgtt cccgagttgg
    89521 cttcacgtgc acaccaacaa aatattgtac ccgtaattga tgttgctcta aaaaaagcca
    89581 acgttaccaa agaacaactt tctgctatag cctttacaca aggtccaggt ttaatgggtt
    89641 cacttttggt tggtacttca tttgcaaaat cgctggcttt ggctttaaac atacccttaa
    89701 tagcagtcaa tcatatgcat gcgcatattt tggcgcattt tattgatgaa gatggctttg
    89761 acaaacctga atttccattt ttggcattaa ccatttctgg tggacatact caaatcgtaa
    89821 aagtcaaaaa cttttttgac atggaaatta ttggcgaaac taccgatgat gctgttggcg
    89881 aagcgtttga taaaactgca aaaatattag gattgcctta tcctggtgga ccattaattg
    89941 accaatttgc caaagaagga aattcaaaaa aatatttgtt taccattcca aaagttaaag
    90001 gattgaattt tagtttttcg ggtttgaaaa ctcaaattct ctattttatt caaaagaacg
    90061 ttgcggaaaa tccaaatttt gtagaagata ataagcatga tatttgcgct tcagtacaac
    90121 aaattatcat tgatatatta atggaaaaat taaaacttgc agtagaagaa actggaatta
    90181 ctcaaattgc cattggtggt ggtgtttcag ccaattctgg aattagaaat acattaaaag
    90241 aaacagaaca aaaatacggc tggaagacat tcatcccaaa atttgaatat acaacggata
    90301 atgcggcaat gattggaatt gttggttatc aaaagttttt acacgataaa tttgaaaatg
    90361 ctgctgtagt gtctaaagca cgaatagagt tttaattatg caattattct acactccaaa
    90421 tttaactgaa actcaaacgt tttttgtttt tgacaaagaa gaaagcaaac atattgtaaa
    90481 agtactgcgc aaaaaaactg gtgatatttt acatgttact aatggcttag gctatttatt
    90541 tactaccgaa ataacaattg cttcggattc aaaatgtacg gttaaaatta attcttttca
    90601 aaaacaagaa gctccaaaat ttaaattgca tttagccgtt gctccaacca aaatgaatga
    90661 gcgctacgaa tggtttttag aaaaagcaac cgaaattggc attcaagaaa tcacgccaat
    90721 tatctgtgaa cattctgaaa gaaaagtaat aaaaactgat cgtttccaaa agattttaga
    90781 aagtgctatg aaacaatctt tgcattatta tttacccaaa ttgaatgaac caattacatt
    90841 taaagatttt atcaaaatgg aatctcaagg tcaaaaattc attgctcact gtgaagaaac
    90901 aactagaaaa tctttgaaaa acgaattgaa aaccaatgaa aatgttttaa ttttgattgg
    90961 tccagaaggc gatttttctg ttaaagaaat ccaacaggct attgctacta aatttattcc
    91021 tgtatctttg ggaaacacaa gattacgaac agaaacagca gcaattgtag catgtcatag
    91081 tgttattttt aaaaatgaaa cgtaaattat gaattcgatt aaaactttat tttctattgt
    91141 agtacttatt gttatttgcc aaattagttt tgctcaaaaa caaacacttt tcattgacaa
    91201 agattttaat gcagttatgc aacaagccaa attagagaaa aaaccagtca ttttaatgtt
    91261 ttatgccagt tggtgtgcgc attgcaacaa aatgaaaaat gaagttttta tcaatcaaga
    91321 tgtaataaat ttttatactc aaaattacat ttgtattgca tcagatattg aaagcacaga
    91381 aggaaaatta cttcgagaaa aacttaaaaa tcaattgatt gtaaaatcat ttccaacgtt
    91441 ttgttacttt gatgacaatc aaaaccttac caactgtata acaggagaat ttaaatccga
    91501 agaatttatt aaagaaggtg tcgataattt aaatgaagca aatcaccttc aaaatgtaaa
    91561 atcaagtttt gaaaaagagg tttctaatta tgacaaatgt ttttcatata tacttatgac
    91621 aaaaagaatt ggatttaatc caacagaaat tgctcaaaaa tatttaaaaa cagttgctgc
    91681 aaataattat tataccgagg aaaattggcg attgattgcc aacggtatcc ttgactttga
    91741 tgcacctgaa ttcattgatt tggtgaaaaa caaagctaaa tttgaaaaag tagtttctaa
    91801 aaaacgtgtt gagaaaaaaa tcaattttgt cattaatgat aattttgctg attatataac
    91861 tcaaaatgac actgttactt ttaataaaaa ccgaaaaatt gctgctaact ttaaactacg
    91921 ggaaattgat tccttattat tccatcaaga tttaagttta tacgaacgaa cgtataaatg
    91981 gaatgaatac aatagcgttt tagaacaaaa catggcaaat tttggtttaa aagatgcaca
    92041 atttatcaat aacgtttgcg ctaactattt catttacatt agtgacaaaa aaatgctaga
    92101 aaacgccgtt tcatgggaaa aaacagcttt agaactcaat ccaagtttag ataaatatgt
    92161 gctaattagc aatttgttgt taaaaaacaa agactacaaa caaggcatcc aatatgctac
    92221 caaaggaaaa gaatttggca aaagtcttgg ttttaacaca actgaaatcc aaaatgtttt
    92281 agacgaatta atagcgaaat ccaaaaaatg aaatcaaata taaaactcta tttattcttt
    92341 ttttcacttg taacatttaa tgcgttttct caagaaattg cattattaaa atacaatggt
    92401 ggtggtgatt ggtatgcaaa tcctacttcg ttacccaatt tgataaaata tgccaatcaa
    92461 acaattaata caaaaatcaa aaataagcca gcgaccgtag aacctggaag tttagaaatt
    92521 ttctcatatc cttttgttca catgactggg catggaaatg tagtttttag tgatgctgaa
    92581 attttaaatt taagaaatta tctgatttct ggtggatttt tacacattga tgataattat
    92641 ggaatggacg aatatattcg tagagaaatc aaacgaattt ttccaaataa tccattagta
    92701 gaaattcctg ctaatcatgc tatttttcaa aaaccaaatg tttttgctaa tggtttgccc
    92761 aaaattcacg aacacgatgg aaaacgtccg caagcatttg gaatatttga aaacaacaga
    92821 ttaatcctaa tgtacacttt tgaaacagat cttggcgatg gttgggaaga tccagaagtt
    92881 cataatgaca ccaaagaagc tcgtgaaaaa gccttgaaaa tgggaacaaa tatagtaaac
    92941 tacattttta acaattaact atgtattcat ccaaaacaat tgcatatgga atcgtaaaag
    93001 ccgttggaat cttgctgctt tttgccttgt tgttattgtt tttttataaa attcaaaccg
    93061 taattttata cctagttatt tcgttaatat ttagcttgat tgccaatccg atagtagagt
    93121 ttcttaaacg aaaactgaaa ttcaaaaata cacttgctgt cattacaacc ttagttctgt
    93181 ttttactttt aattattggt gtcatttcca tgtttgttcc tttaattatt tctcaaagca
    93241 gtaatttatc attattaaat acccatcaaa ttgaaaaaaa tgttctagat atagcgacac
    93301 aattttctca atttctaaaa aaacacaata ttgaaagtga aaatataata aacccaaatc
    93361 aatggttgtc caaaatgaat tttgccgttt ttaccaattt tttcaattct ttattgacta
    93421 ctattagtaa tatcggaatg ggattggctt ctgttttctt tattactttt ttctttttaa
    93481 aagataagtt gctcttttta tcttctgcaa aagcaatttt acccgatgct cacgaagacc
    93541 aaattttaaa ctcagtaact aaaataaacc tattactttc tcgctatttc attgggttaa
    93601 tcattcaatt ggcgcttatt tttattttat atcttatcgt tttagtactc tttggcgttc
    93661 aaaacgcatt aattattgcg ctaatttgtg gtattttgaa tattattccc tacatcggtc
    93721 caataattgc tagtgtatta gcagccgttt tgaccatgat gggtacgata aatagtgatt
    93781 ttcaaaccgt aactttgcca acaaccatct atgttttaat tggttttttt gttgttcaat
    93841 taatagacaa caacatcaac caacctatta ttttttctaa cagtgtaaaa tcacatccat
    93901 tagaaatatt tcttacgata cttattggcg gttttatttt tggaattgtt ggaatgatta
    93961 tcgctgttcc tttttacacc attctaaaag taattggcaa agagttttta cccgaaaaca
    94021 aaatcattaa actttttaca aaaaatattt aagaatgaat aaaaccattc tttccgaaga
    94081 aattcaagcc tttattaatg agcatgtttc ggcaaatgtt agtgatttgg cattaaaaaa
    94141 atctaaattt ctttcttttg attggactct aattttaaat caaattgaag ccaaacagaa
    94201 agcaaaaata aaattaccca cttggttttc aacaaaaaac atctattatc cgagtaagtt
    94261 atccattgag caaacttctt ctgagaaaac tgctgcatac aagtcaagtt tagtttcagg
    94321 cgaatcttta attgatttaa ctggtggttt tggcgttgat gattactatt tttctaagaa
    94381 aataaaacat attttacatt gtgaaataaa tgcagagtta tccgaaatag taacacataa
    94441 ttttgaagtt ttacaacagc aaaatataga atgttttgcc gaagatagtt tgactatttt
    94501 agataaattg aaacgaaatt ttgattggat ttatattgat ccctcgagaa ggaatgatgc
    94561 aaaagggaaa gttttcatgc ttaaagattg tcagcccaat gttcctgaat tattagattt
    94621 ttactttgaa aaatcaaata acattatgat aaaaacagca ccaattttgg atataactgc
    94681 tggtttatcc gaattgaaaa atgtaaaaga gctacatatt gttgctgttg ataacgaagt
    94741 aaaagaatta ctttggattt tagaaaaaaa atataattca aatataaaaa taattacttg
    94801 taacattcta aaagaacatc aagagttatt ttcgtttagt ttaaatgatg attcttcaaa
    94861 ttttgattat agcttgccta aaaaatattt gtatgaagca aatgctgcca taatgaaatc
    94921 aggtggtttt actgaaatcg gaacacaatt tggaatcgaa aaattacacc aacattccca
    94981 tttgtttaca tcagatactt taattgattt tcctggtaga gttttcgaaa ttgagacagt
    95041 catgaattac gagaaatctt ccatgaaaac ccatttatca aacaaaaaag caaacattac
    95101 cactagaaat ttcccagaaa ccgttgataa tattcgaaag aaatggaaaa ttcaagatgg
    95161 cggaaatttg tattgttttt ttacaaccga tataaataac aacaaaatag ttttactttg
    95221 caaaaaatta aaataatcat gaaaaagttc atttctatag tacttctact tttaaatttc
    95281 tttgctttcg cacaaaaacc gtgtgaaatt gacactgata ttacggatag tattggtact
    95341 tataaatcaa caaaacaatt tttgattttt gaacgcaatt ttgccggaaa ctcaacaaat
    95401 atttattttt ctttagcttc taataatggc attttaaatc ttgaatttca atcgattcaa
    95461 agaagtagtg actttattaa agccaattgt tttgataaaa gttctaaaat ttatcttcaa
    95521 ttaaacaatg gaaaaattgt tactttatta aatgttggcg cagatacttg tggcacttta
    95581 ttgcgaagtg atgatggcaa aaacaatcgt atcactagta gctcgtttgt ctttacaaaa
    95641 gaaaattttg aagatttaaa aacctccaaa attactttta tgcgtattca attttctgga
    95701 gaaattatag attatccatt taaaaccgaa ttagtttctg aaatggacaa taaaatttac
    95761 caacccgaat cttattttat tgattatttg aaatgttttg actagtaact tctgttaaac
    95821 ttcatcgcta gtaaaagtta caaaacttcg ttttttgtaa ggtctaataa ttaatctgcc
    95881 ttctctgtca aaacgtatgt aattgtaaac ccagttaaga aacacaacgg ctttgttttt
    95941 aaaaccaatt aaggaaaaca aatgcactat catccaaaca aaccaggcaa aaacgccact
    96001 aaaatgatat tttggcaaat caacaacagc caaatttctt ccgattgttg ccatcgaacc
    96061 tttgtcatta tattgaaaag gtttcatcgg tttgttttta attaacttta taatgttttc
    96121 gcctaaaagt ttcccttgct gaatggcagg ttgtgccatc attggatgtc cttttggata
    96181 atctttagtt tccatagaag caatgtcgcc caaagcaaat atgttttcat agccatttac
    96241 ttggttgaat tcatttactt tgatacgttt tacacgttca acaaaagcgt cactatttaa
    96301 acctgaaatt ggtgcgcctt caacacctgc agtccaaatt actgttgctg tttcaaaact
    96361 caaatcagaa tttgtagaaa ccgttcttcc atcatagttg gtaacacgaa cgtttttcca
    96421 aattttaaca cctagtttat ttagaaactt ttcggctgca attgaggact tttcactcat
    96481 agtactgagg actttatcat cactttgaat caggttaatt cccattttag atatatccaa
    96541 atcgggataa tctttttgga gaattgcttt tttcatttca gccaaagcac ctgccaactc
    96601 tacacctgtt ggtcctgcac caaccaaaac aaaattgata agagcatctt tttctgactg
    96661 atcattggtc aaaacagctt gttcaaaatt ttctaaaatc aaactcctaa tatttaaaga
    96721 ttgcggaatg gttttcatgg acatcgcatt acgttcaatt tctttatttc caaaaaaatt
    96781 ggttttcgaa cccgttgcaa tcactaaata gtcataatgc aaatcgccta tttctgaaat
    96841 tattttttgg tttttggtat caatttcttt aactgaagtt aacctgaaat agaaatcatc
    96901 atactcttga ataacttttc gcaatggata ggctatagaa cccgcttcca atccgccagt
    96961 tgccacttga tacaacaaag gttgaaaagt atgataatta tgtttatcga taagaactac
    97021 ttgaacttct ttgtttttta atttctctgc tattgcaatt ccagcgaaac caccaccaac
    97081 aattactatt cttggtttat ctgcgtgagg tatattcata gactaaactt taggaaaagt
    97141 tacatcaccg ttccaattac tgaaacctcc aattaaattg taagccgttt caaaacctag
    97201 ttgtttcata atcccgcaag cttgagcact tcttccacca gctttgcaat agacataata
    97261 attttttgtt ttatctagtt cttcaagttc ttcaataaaa tcatgtcctt tataaatgtc
    97321 tatcattatg gcgtttggaa taatgccttc atcacattct tcttttgttc taacgtcaag
    97381 tattacagca tttgtatcat tttttagtag cgatttccaa tcttcttgta tcaaatccat
    97441 agttttctat ttttatcaaa tatactaaaa aaatgacttt tcagaaagta tatcactaaa
    97501 ggaaaaataa aactttaaca aaaaaataac agaagcttgt atatacaaat aaaataaagc
    97561 attacatttg tacctacaaa tattgagtgt gtaacaatgg aaattgaaaa aataataaaa
    97621 tcgacgataa acataagtgc agcaaagaaa gttattctta acatggtgta caccaacaca
    97681 cttgttggtg aaaagttttt agaaaaactt aagccttatg atttatcgag cgaacaatac
    97741 aatgttttac gcattctgag aggtcaaaaa gggaaaccag ccaatatgtg tgtaattcag
    97801 gagcgaatga ttgctaaaaa cagtaatacg actcgattga tagataaatt attattaaaa
    97861 caattagtga ctcgcgatgt ttgtcctgat aatagaagaa aaattgaagt attaattaca
    97921 caaaaaggtt tggatttatt gacagaatta gatccaatcg ttattcaaca tgaagaagat
    97981 tttgcttcaa atttaacttc taccgaatta gaagaattaa atagattatt agaaaagtac
    98041 agaaatacaa ttaactaaat tatgtcaaac tttatacaag atgccaattg gcgttatgcc
    98101 accaaaaaat ttgatgcatc caaaaaagta tctaaagaag atttagatac actaaaagaa
    98161 gccatacgtc tgagttcttc ttcttatggg ctacaaccct acaaggtgtt cattatagaa
    98221 aaccctgaat tgagacgaaa gttgcaacct gcggcatgga atcaaagcca aattgtagat
    98281 gcttctcatg tagttgtttt tgcgaatgtc gttaattttg gtgatgaaga aattgataat
    98341 tacattcaaa atttaattaa aaccagaggc atcgaattgg catcagttca aggttatgct
    98401 gattttatga aaatgaaaat caacccttta tcaatcgaag aaaaaaagaa ttggacatca
    98461 aagcaaacct atcttgcttt aggaaacttg ctaaatgccg cagctgaatt aaaaatcgac
    98521 gttacgccaa tggaaggctt tgaagaagat aaagtaaacg aaatacttgg actaacccaa
    98581 ttgggcttaa atgcaagctt aatcgctact attggctatc gacacgaaga agacgctact
    98641 caacattttg ccaaagtacg caaatcaaac caagaattat ttatcaattt ataatcaata
    98701 actttaaaat caaaaatcta tgaaaaatgt aaaaacaatt gccttagcat tagtaattgc
    98761 tttaggaaca acagtagctt cggctcaaaa caaaaaaata aacattgaaa aaagtaaaat
    98821 caactgggtt ggaaaaaaag taactggaca acattcaggt acaatttcct ttgaaagcgg
    98881 tactttagtt tttgaaaaaa aagctttaaa aggtggttcg tttgtggtgg atatgtcatc
    98941 aatcaatgta actgacttag aagctggtaa aggaaaagaa aaactggaag gacacttgaa
    99001 agcagatgat ttttttggta cagataaatt tgcgacttca acattagatt ttaaaaaaat
    99061 agcttctaaa ggaaacgacg tttacactgt aactgctaat ttaactatta aaggaattac
    99121 aaatccagtg acatttgaca tcgctgttaa aggaaatact gcaacaacca aagtgattgt
    99181 tgatagaact aaatttgaaa taaaatatgg ttcaggaagc ttttttgaca acttaggaga
    99241 caaaactatt agtgacgaat ttgaattaaa cgttagccta gtattctaag tcaagtacca
    99301 tattgctatg gaaaaggaaa tcctcaaatt aatttttgag gatttttttt cattttaaaa
    99361 agaaatgtta gaacactatt tctgcataaa acagtaaatt tgtaccaatt caaatcgcta
    99421 tgaaaaaaat tgctgttatt gacaactacg atagttttac ctataatttg gttcattatc
    99481 ttgaggattt gaatgctatt gttaccgttt ttagaaacga tgaattggaa cttgaagaac
    99541 tagaattctt cgacaaaata gtactttctc ctggtccagg aattccagat gaagctggat
    99601 tattgaaagc tatcattcaa aaatattata aatctaaaag tattttaggc atttgtttgg
    99661 gacaacaagc catcggagaa gtctttggtg gcactttaat taatctcaaa aaagtacatc
    99721 acggagttgc ttctaaggta aaaataaccg ttccaaatga agagcttttt aaaaatttac
    99781 ccgacgaatt tgaggttggt cgttatcatt cttgggtgat ttctagagtt aattttcctg
    99841 aaaatttaat cataacttct gaagataaaa acggtgaaat catgtcaatt tcacacacaa
    99901 tttatgatgt aaaaggtgtt caatttcatc ctgaaagtat cttaacacca cttggaaaaa
    99961 agattttaga aaattggatt atgtcttaaa atattttttt atattgaatt gattttaata
   100021 aatttaaatc aatcaataac aacaaacact ttatgttgat tcgttttcac cacataaacc
   100081 ttaaaaatca acaattttag caaaattgtt actcaaaaat gtgttttgaa agtaaataat
   100141 aagtttgtta caattgattt ttaacttcgt ttttcaaaat ttcatcattt tcactttttg
   100201 atgcctcaaa gaaacaacat aaaaacttat actttatgta atttaattta aaaaaatgaa
   100261 taacatataa ttaacaatgc ttaatagata ttatctataa ctcgttttta gttaacattg
   100321 tgttaaaaat aaattaaaca aacgcttaat ttaaacattt gtttaatttt gtactcgatt
   100381 taaacttcta aaaaaagcat gactgaattt aacgaaaaac aaattgaaat tttacaagct
   100441 gcagaaaacc tattttctga agtaggtttt gacggaacaa cagttcgagc aatttcaaaa
   100501 aaagcgaaca taaatgttgc tatgatttcg tattattttg gttcaaaaga aaagctttta
   100561 gaatcattag ttatctacag aatttcagga atgcaaatgg acttggaaaa tcttttttta
   100621 gagcacatct ctcctattga aaaaattgaa aagttagttg ctttttatgt tcaacgaatt
   100681 tattccaatc ggtgcatgta tcaaattttg cattttgaaa tgtctaacaa aaaaagagat
   100741 ttggatttaa aagcatttac cgatgtaaaa aatagtaacc tccaactttt agcaaatatt
   100801 attcacgaag gtcaagcatt aaatcttttt aaaaaaaatg taaccatcga attaattccg
   100861 ccaacaatta ttggaagttt tactcatttc tacatgaata aatcttatta ccaaaaatta
   100921 cttggattaa ccacagatgt gttattagaa aactacatca ctacgacatt tacagaacat
   100981 cttcaaaaaa ccatcaaagc acttttagta tatgaaaatt aataaaatga tatttagtat
   101041 catccttgct ttagttttta acaacatcga ggctcaagaa aaaaagctac ttacattaaa
   101101 agaagccatc caaattgcag taaccaatag cgaccaagcc acattggcta aaaccaaagt
   101161 aattacttca aaattggaat tagacaatac caaaaacaat agctatccaa gcttgaaagc
   101221 cagcggacaa tatttaagat tatcgaatgc taatattgat tctaacattc aacccaactc
   101281 gagtgcttct ggcgaaccaa gtaagccttt aaaagcggaa cagcttatgc tcggacaagt
   101341 aaatgcttcc ttgcctattt ttagcggttt taagctaaag aatagcatca aaacatctga
   101401 aagcttgttg gagtctgaaa aattcactga aaagcactca aaagaacaaa tcggactaca
   101461 agttgttgaa ttgttctctt cgttatacaa aactcaagaa atgacgctgc ttattgaaga
   101521 caatctgaaa acagccaatc aacgggtcaa agattttact gccatggaag aaaatggttt
   101581 gattgcaaga aacgatttat tgaaagcgca attacaagta tccaacatac aactggcatt
   101641 agacaattcg aagaaaaata ccgctatttc caattacaaa ctaataacgt tactaaaact
   101701 tcctgaaaat acaacgatag aaattgacat tgaagccatc aaaaaagaac tgaaagaaaa
   101761 tcagaaagca ttttcgggag aaagaaacga cttgaaagct ttagaatata aaaaagcagc
   101821 atctgaaagt gccgtaaaag tagctaaagg aaactattat ccatcgatag ctttatcggc
   101881 aggatatatt gctttggaca tcaacaatat agtaacggtt acaaatgcta tgaactttgg
   101941 tgttggtttt tcttatgaca tttcgtctat tttcaaaaac aacaaacagg ttaaacttgc
   102001 tcaaaataag actaaagaaa ctgaaatcgc tatccattta cttcaaaatc aaattaacga
   102061 agacctattt gaggccaaag aaaactatgc actttcgtta aaacaaagtt tggtttatga
   102121 aaaagccgta gaacaagcta ctgaaaatta cagaatcgta aaagacaaat atgacaatag
   102181 cttatcagac acaaacgact tgcttgaagc cgattttgaa gctttgcaag ccaaaataaa
   102241 tcaagcctta tcaaaagccg atgtagctca aaaatattat gaattacaat ttgccaacgg
   102301 aactttaacc tcatcactaa acatcactca aaattaattt tgcaacagat atggaaacta
   102361 aaaaaacaaa taccaaattc aaacttatat tcatagtact cttagttatt ggaatatcct
   102421 acggcggtta taaattcatc aagtcattat cccatgaaac taccgacgat gcgcaggtag
   102481 agaaaaagat gaacccaatt atccctagag ttccagggta tattaccaaa gtttatgtaa
   102541 aagacaacca acaagtaaaa caaggtgata ccctatttgt gattgacaat agtgattatt
   102601 tggttaaagt agaagaagcc aaagctgccg ttgccatagc agaaaacagc atggaagttt
   102661 ctaaagctga tattggcact tcgcaagctg gtatttctgt ctcgaatgct aatgccgatt
   102721 ctgcattagg ctctattgaa acagcaaaaa ttagacttcg ccgtgcttcc aatgattttg
   102781 acagatacag taatctgtat aaaaaccatt cgattaccaa acaacaattt gaacaagccg
   102841 aagctgcaaa acaagaagct gaaagccaat tgcaaatcat ccaaaaccaa caaaaagcat
   102901 cttcttatca aagaacggtt gctgctgcac gaaacaatgt tagtacaaaa caagtgcaag
   102961 tagcacaagc taacattcaa aaaaccaaag ccatgcttga tgctgctaac ttaaatttaa
   103021 gttataccgt tgttaccgct agcattgacg gacaggtttc tactatcagc attcaaccgg
   103081 gtcaattggt gcaaccagga caatcgttat tttatatcat caacaatcaa gaaacatggg
   103141 ttgtggctaa ttttaaagaa acacagttgg acaaaatgcg cattggtcaa aaagtaaaac
   103201 tacaagtgga tgcttttcca gatgttactt ttgaaggcga attggcttcg ttttcgcctg
   103261 ccacaggctc taagttttcg cttttaccac ccgataatgc cagtggaaac tttgtgaaaa
   103321 ccattcaaag gcttccggtt agaataaact ttacaaacca aaacgataaa gaaaaactac
   103381 aattacttcg ttcaggaatg aatgtagcgg tagatgtggt tttgaagtaa gttggaagat
   103441 ggaagtttgg agttagaatt tagaagttag aaaatggcaa cagcagctgt agtacaagaa
   103501 gatttagtag aatatggttt tagacgcgta attattacca ttacggcggt tatgtgtgcc
   103561 ttgttggaaa tcgttgacac cactattgtc aatgttgcgt taaacaacat gcgtggtagt
   103621 cttggtgcaa ccttaactga cattgcttgg gtaattaccg cttatgctat tgcgaatgta
   103681 atcgttattc cgatgacaag ttggttgtcg caacagtttg gacgacgcaa ttattttgct
   103741 acttctatta ttactttcac cgtagcttcg tttatgtgtg gtcaagctac taatatctgg
   103801 gaattagttg cctttcgatt tattcaagga ctgggtggtg gtgcgctatt ggtgactgct
   103861 caaaccatta ttaccgaaag ttatcctgtt gcaaaacgtg gaatggcaca agctatctat
   103921 ggaatgggtg ttattgttgg tccaacgctt ggtcctccat tgggtggtta tttagttgat
   103981 aattattctt ggccttatat attctatatc aacattccta ttggaattat cgccactttc
   104041 ttgacactta cttttgttag aagtccaaaa tatggaaata aactaaaagc caatcaagtg
   104101 gattggttgg gtatcttttt gctttcggct tttgtgggtt cgttacaata tgttttagag
   104161 catggacaac aagacgattg gtttaacgat gctgctatcg ttgcgctatc ggcaactagt
   104221 gtgtttggat tgtttttctt catttggcgt caaatgactt atgagtttcc tattgtaaac
   104281 cttagggtgc tcaaagatgg aaaccttcgc attggaaccg ttatgacttt catcttaggg
   104341 tttgggctat atggttcaac ttttattatt ccgctttata cacaatctat tttgggttgg
   104401 acagctaccg atgctggttt aatattagtg ccaagttcgt taaccactgc tttcttgatg
   104461 ccgtttattg ggcgaatgat tcaaaaaggt attccgcaat cctatatggt agcggctgga
   104521 tttatgattt tctttatctt ttcctattgg atgcacgtag aaatgacacc cgacactggt
   104581 gttgaacatt tcttttggcc attaatcttg cgtggtgttg gacttggatt actcttcgta
   104641 cctattacaa ctttgtcact ttctactttg tcaggaaaaa gtattggaga aggagctgcc
   104701 atgacaggaa tgatgcgcca acttggtggt tcattcggta ttgccatcat tacaactttt
   104761 attgctcgat ttaatcaaga acacagagta aatcttatta gccatcttga tccaactaga
   104821 gttaatgtgc aagatagaat tgcagcactt caaagaggat ttatgtcaaa agggtttagt
   104881 tataacgaag cattagacaa agcttataaa gttcttgact ttacggtttc caaacaaagt
   104941 actgtattgt cttatatgga tgtgttttta tatttaggaa tacttttttt agcctgcatc
   105001 ccaataacgc tattgattaa aagatctaaa aacaaagtag atttgagtga agctatgcat
   105061 taacaatcta atgatttgca agttattaac aatttattgt cgttaagata atattattta
   105121 actttgaaat tgacgttact ttgtttacta acttaaattg aatgaatcat gggcattttc
   105181 aacaatcacg ataagaaagt aattgcgcaa ctttgtaaaa aaagcgaaga tattagcaac
   105241 gaaataacta aagaaatcaa tgagtttata gacgatttga attcggagta tgaagaaact
   105301 aaagtagccg ttaccgaatt tagctctttt attagcgagt tggaaaccaa actttctcct
   105361 gaggaaataa acaaactcca ctccttctct actcgattga acaaagtaaa acgttgtgcc
   105421 aaaaaaggcg tggaagcaat gcgcgaattg gctcgcgacc aacgaaaagc tactagagaa
   105481 accattcgcg agtatgaaca atacctttat atatagttta tcgtgtaaaa actaaagtat
   105541 ttcctttact caaattggcg tcaaaggagt aaccctcata attgaaccct tttaaatctt
   105601 caatagtttt ggcattggtg tcgataatgt agcgcaccat catgccacgg gctttcttgg
   105661 caaaaaaact tatcatcttt aactttccgt ctttatagtc cttgaactct ggtgttacca
   105721 ctggcacctt tagtgtttta acatcaactg cgctaaagta ttcgttgctg gcaaggttaa
   105781 caaacaattc gtctttctct aattcttcat tcaaagcttt ggtgatggtc tttttccaaa
   105841 aggcatatag gtttttatgt gttccgatgg tgattgtcgt tcccatttct aaccgatagg
   105901 gttgcattaa gtctaacggc tttaacaaac catatagccc tgatagtatg cgaagtttag
   105961 actgcaaggc ttctagtttt gcttcggaca aagtgtaaac atccaatcct gtataaacat
   106021 cgccatcaaa agcataaacc gcaggacggg cattttgggt agtaaatggt actgaccaat
   106081 cttgattgcg ctgccaattt aattccgcca acttatccga aatagacatt aatgccgaaa
   106141 gttgtttggg ctttttcttt tttaaagttt tttgaatggt aacggcttgc tttaaaaaag
   106201 cgggttcagt atgctgcgta gtaggcaatt ctttttcaaa gtttaacgac tttgctggag
   106261 agattacaat tttcatgacg tttgggttgt gttctttttc aattattcaa aagtaatgaa
   106321 tggtttttaa agttttatga ttgtttaaaa gtttaagagt tgtttaaagt agtttaagag
   106381 gttaaaagat tttggaacgc gaattgaaaa aagtaaagat atttttaaaa ttaatcaatt
   106441 gccattgttt tgcctcctcg attaagaaaa ggtcacatca caccaatcat ccaacacacg
   106501 ccaaagttcc acaggaacaa ccgctattct aggcagtcat ttcagcgcct ctactaaatg
   106561 aagttgtttt tttgagttat taaacttttt tcgtttatgg tagtggttgc tcaacagcta
   106621 ctgctgtaat aatcaggtca actaatttaa agttggatcc tttggtttga atcgtggaat
   106681 taaagattct cctgaaaaaa ccattacttg caatacaagt aaaattatga tgatgattgc
   106741 aagagcaaat tgcgatgatg ctttgattgt ggcatattgc gcttccactt gtttcattat
   106801 a
//
LOCUS       DAOOBH010000002        69646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_463_length_69646_cov_7.375207, whole genome shotgun sequence.
ACCESSION   DAOOBH010000002 DAOOBH010000000
VERSION     DAOOBH010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 69646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69646
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_463_length_69646_cov_7.375207"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(622..2631)
                     /locus_tag="PK218_00525"
     CDS             complement(622..2631)
                     /locus_tag="PK218_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW97025.1"
                     /translation="MKKILLLFLALVCITCEQKKNASSDSKQDSIQKYIDLAANDTID
                     YKDRVKYNDLAFEIAINQKNTFRNREYLSKIGFNNYHLNNLKGFEKTYKYLLDKSFET
                     KDSVNYGNALRMKAFHLQINNKLDSSLLLYIKAEKVYKALNKPKEYALVLLNKSLILY
                     DIGDYNACELTLSKSLFYIKKTNDKKYYYQILNQFGVLYSELGEYDKSLKFHIDALKI
                     VEKSKIDSNDILYSSCFNNIGNVLLKKKDYKNAIKKFKIGLENKTLINKDPELYALLN
                     DNLGYSYFNENDYLKALNCYKKASSVINKINYKPILINIYIHLSEYYIRNGNRNRAII
                     YSELALNEAKKSKIYSNLLLALKSNMFADNKKTEANLEEYISIYDSIQLSNRKSKDLF
                     AKIELETDEISQQKEIAEKNQFIMAGLSVALFFVGILFFIIIKQRSNRLKMELEQRQQ
                     KANEEIYQLMLSQKNSNEAAKEAEKKRIALDLHDGIMNKLASTRLNLSILAEKSDETT
                     ITKCLTHISDIYAIEQEIRVIAHNLNNEVFQQENSFVSLLQDFVAVQNETTDTHFLLE
                     LDEAIVWNAISSEIKMNLFRIIQEACHNSNKHANANKIIIAFILDENNLCLSINDNGV
                     GFDTQKSSNGIGIQNMKTRVHALGGKITINSFQNHQTSINISVPV"
     gene            complement(2718..3119)
                     /locus_tag="PK218_00530"
     CDS             complement(2718..3119)
                     /locus_tag="PK218_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97026.1"
                     /translation="MKFKRLLTIVLLLPMMSFAQSDLDKVLKGGELLLGGISIFKVAK
                     SDGKKDSKLIESVCVKNKRTDRITFRIVGKNEEGDEVKKELVVPKDGKECVFELPKGI
                     YTYEIVLANKEILKKGEYKFEEEVIITVRDE"
     gene            complement(3373..4158)
                     /locus_tag="PK218_00535"
     CDS             complement(3373..4158)
                     /locus_tag="PK218_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97027.1"
                     /translation="MKNLFFGIIATVCFTSISFSQSINVNTIGDFHNVQMEKYYEKLK
                     TFTETEGKEKITQSDFKLLFKEVLISVYGNENPSIVNESLEGFDNCFGKTSIEIADLS
                     INKIQSANYKNEELKLFLINTINDCKVLEFEKSKSYFDNKIIEANKKFEDKDLEIALS
                     TLNTGKASFNYWEENYSKWHLLFNDSSTSASRGGAVRAGRAIGAADCAGFCGGALWGA
                     MGGTAFLPGVGTVTGALAVGCANGMYASVVAGLGCAFLSLFGG"
     gene            complement(4467..4667)
                     /locus_tag="PK218_00540"
     CDS             complement(4467..4667)
                     /locus_tag="PK218_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97028.1"
                     /translation="MELKKLLALAFLLPIMSFAQSDLDKVLKGGELLLGGISIFKAAK
                     SDGKKDSKLIESVCVKNYFSLF"
     gene            complement(4900..5175)
                     /locus_tag="PK218_00545"
     CDS             complement(4900..5175)
                     /locus_tag="PK218_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97029.1"
                     /translation="MKLDKEKTFTFSQERVLIYTGVNENGITKITPNVQNRSAFGNCM
                     DAVENDFTDSLVGWIYWNANPQVQALAAGLCYTCTRWGKPCPAAYPN"
     gene            complement(5526..8180)
                     /locus_tag="PK218_00550"
     CDS             complement(5526..8180)
                     /locus_tag="PK218_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97030.1"
                     /translation="MKKILNALFLLLVSISYSQNIIRGKVINQDKEIIENASITIEDP
                     SDSSVLAFSITDKNGDYRIDIESTNLKLNLVVNAINYEKIIQSINSKSQVLNFTLKNK
                     ITELEEVKIDIKEIQKRGDTLTYDVKSFEGKEDRTLSDVLKKIPGIEVDANGLIKYQG
                     TAINKFYVEGKDLMAGGYGSLTNSMPKDAVTKLQVLENHQPIKALKNSVPSERAAINI
                     KLKKDVTLTGRADTGVGLSPFLWSAKLTPMVFTKKYQYLLNYKSNNIGEDVTYELQTL
                     SFDEGFEGLTFENQTGNWLNVSQSELPKIDENRYLFNKTQLFSANLLTDVSKNWELKA
                     NTNFYNNKINNNGNQYSKVNIFDAFGNLQNTIEYNRLNNSSTENNQFKSQIIFTKNSD
                     KSFLKNTTTYKGNWNTIQGSTLINSSNTNQYVSSPGYSFQNSLSAIFLVGKKLVNFKS
                     TFNYINDKQNYFVEPLNNINIPQFSDINAKNVNQNVIDKTISMTNEASYIFSIKKLSI
                     IPSIGFEIEAKNLKTNFNGENSSNETISFGNNFSNNMVWKKHISSSSFSINYVGESLT
                     FNTNLPLKYYSLNAKDNNLNFHRNLNKLTFEPNFSTKYKFTTELSNTLYGSINNNFGT
                     LNSIYPSYIFSALNFTNQNSDIQQSISQKIGGNFEYKLILYNLFLNLNYQLSQDSSNN
                     TISQNVMDNGQTNFQQISSKNKSINRNANIEISKYIPKIKSKVSLEYGISSSSNNIIL
                     NNNKLLINSTGNTFTFKANNNYYNWLTFDYNLFYSINQRGNINKTNTLKSNLKILLYP
                     VSNQTFGIYRDDYIYQFNNQNFRNQFIDLSYQYTMEKRKIDFELKWTNILNTKTYEEV
                     ILNDLGYTSNIIVIRPSQIFASVKFNFN"
     gene            complement(8200..8991)
                     /locus_tag="PK218_00555"
     CDS             complement(8200..8991)
                     /locus_tag="PK218_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HPW97031.1"
                     /translation="MFKYYYILFTCLFTFGQQKINITNEARYELTFKEYKNQKPEFLK
                     NTFILLFNEKESFLKNMSLYVRDSLVDNGKIKKTGDSQKDFPVFAKYVPDLPYNIYKK
                     ENTISFSNEIPFSGEYQYTETIDFEWKITKESKTINGIKCIKATTTKWGRNWIAFYSP
                     KHPLPFGPYKFFGLPGLIFEVYDDAKDYTFTLYKFKNRVANNVLMHNYPKAKKVTKKQ
                     YEKARKVASVKPSTIKIEGYPNINKKIDKYSIDKEKNYNPIELTD"
     gene            complement(9063..9260)
                     /locus_tag="PK218_00560"
     CDS             complement(9063..9260)
                     /locus_tag="PK218_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97032.1"
                     /translation="MKKVFLLLAVFLFSANTFAWTGYFHTSCGITVHVLGTDGESSVV
                     KSLQKINGIFCGTENVAITFY"
     gene            complement(10281..10478)
                     /locus_tag="PK218_00565"
     CDS             complement(10281..10478)
                     /locus_tag="PK218_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97033.1"
                     /translation="MKKIKLLIILLSFVQYSFAQSDLDKVLKGGELLLGGISIFKVAK
                     SDGKKDSKIIESICVKNFISF"
     gene            complement(10695..11111)
                     /locus_tag="PK218_00570"
     CDS             complement(10695..11111)
                     /locus_tag="PK218_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97034.1"
                     /translation="MKPESIGEPKIFVENNSFKSEPFETSSRMFSFYITVESDFVESI
                     GSGCWTVNVRVYFNTEDGKVLMANQNVNVGSGCPQNRMSIETATNCPTTEFKGDIILE
                     GKDSYKYCLIELLKDDAIYNVYERMKKEFIKRYEQK"
     gene            complement(11881..12540)
                     /locus_tag="PK218_00575"
     CDS             complement(11881..12540)
                     /locus_tag="PK218_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW97035.1"
                     /translation="MIVEVLMIDDHPTIIEGYKAILVFGIKDYQLEVAEAFTGQAAFE
                     IITTTQKRFDVALIDLTIPSYLEKNIASGEDLIPLIKKHHPSCKIIILTSHSESIILQ
                     RITKEYHPEGIFVKSDFQAKELISGFDAIVKEKTYFTQTIVSFQKTWNEKSNLEFDTY
                     NQQILALLNQGVKTKRLPDLLHLSQSAVDKRKAVIKTILGIDKGNDEDILKAARKKGL
                     I"
     gene            complement(12574..13932)
                     /locus_tag="PK218_00580"
     CDS             complement(12574..13932)
                     /locus_tag="PK218_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW97036.1"
                     /translation="MKHSYIIVDDNHESILNTQATAMRFQNLAYMGYATNYEAALNLI
                     LEHQPSLIFLEINPLKKESNLSLNLINELYRYLTIVPKIIVTSKDQSLVFRAMEYGVA
                     DYLMKPLELNAFRKAIFKFDKLVSQNTVSNSVVEQQTVPQQALTFSDFLRQEPIQKPV
                     EKPIVEEVEVKKEEPIDAAEIIVDAAVFETGIIESEANGANESVVEDVIEDKVTDEET
                     NTPEKEEETLEETKDSIENEASEESNDEALEIATSEEETEIANEVLTQEENSTKETIE
                     ESETIETETPEEPISREEVVENTKPLEAKTIEDAVSDDETNDDEAITTLPGQKVIVAQ
                     EDPLIICVKSYGDYRYIDSRDIIYFEADNNSVDIHLKDGEMVTAFKTLKAFESVLPPT
                     QFLRIHNSYILNVSQVARIHTGNNVCYIKNSTIKLPFSKSYKENVELIIHRIASGNYL
                     EI"
     gene            complement(14194..15735)
                     /locus_tag="PK218_00585"
     CDS             complement(14194..15735)
                     /locus_tag="PK218_00585"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPW97037.1"
                     /translation="MAKQEDIFKNVISHAKEYGFIFPSSEIYDGLSAVYDYAQNGVEL
                     KKNIREYWWKAMVQMHENIVGLDAAILMHPTTWKASGHVDAFNDPLIDNKDSKKRYRA
                     DVLIEDYAEKLNLKAKKEIEKAKVRFGEAFNEQEFITTNPRVVEYLAKERQILERMGK
                     SLGDGNLEDVKALIEELEIACPDSGSRNWTDVKQFNLMFGTKLGASAESAMDLYLRPE
                     TAQGIFVNFLNVQKTGRMKIPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQFFVKP
                     GEEMKWYEYWKETRLNWHLSLGLGKENYRFHDHEKLAHYANAAADIEFNFPFGFKELE
                     GIHSRTDFDLKAHEQFSGKKLQYFDNETNSNYVPYVVETSVGLDRMFLAVFATALQEE
                     TLEDGSTRTVLRLPSVLTPTKAAVLPLVKKDGLPEVAKNIIEDLKWDFNVAYDEKDAV
                     GRRYRRQDALGTPFCITVDHQTLEDETVTIRHRDSMKQDRVRISELRNIIASEVDIRN
                     WLMKM"
     gene            16079..16762
                     /locus_tag="PK218_00590"
     CDS             16079..16762
                     /locus_tag="PK218_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPW97038.1"
                     /translation="MFKHLINLFFPKSCAACASFLLAEEIVICTGCRHKLPLTNHHLM
                     EENEVVKKFYGRIPLEFGGALFYFHKKGMVQEMIHKLKYKGHEEIGEMAGNWYSGELK
                     TVEQLKSADYIIPVPLHKKRLRERGYNQVAQFGKTLSANLNIPYNDSVLIRKVYAKTQ
                     SQKNLLGRIDIADTTFDVVFDESFHGKHFVLIDDVITTGATLESCSRALLKIPNVKIS
                     IVCMAMTHN"
     gene            16844..17161
                     /locus_tag="PK218_00595"
     CDS             16844..17161
                     /locus_tag="PK218_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97039.1"
                     /translation="MKKVFYLFSIVFLMQSCFSYKEIENNSSQYEIGKTYRVYQDKKK
                     SIINIKNKTDSTLIVVSKFEEHKFPINSISKVDKRKFSIVKTLLLPVSIVSSIIILFA
                     LTY"
     gene            17257..18858
                     /locus_tag="PK218_00600"
     CDS             17257..18858
                     /locus_tag="PK218_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPW97040.1"
                     /translation="MNRPYFKLFLLLVALAVSSCAKRGTITGGAKDTLAPVLKASFPK
                     NGSVNFQGKEIKLVFDEYVKLKGISKQLIVSPPMKTPPEIFPQNASKTITIKIKDTLQ
                     PNTTYSFNFGQSIEDNNEGNPYSQFKYVFSTGSYIDSLKLNVKIKDALEKKSDNFVTV
                     MLYEKNEKFNDSTIYKETPRYITNTLDSLKLVTIENIKAGKYKLIALKDNGNNKYNPK
                     SDKIGFYSGEITIPNDTIYELELFKESLPFKAIKTYQASGNRLVVATEGNAKNTKVTI
                     KNGNDVLPTVVTQFPKKDSLQVWYKPMKVDSLLVSVEKNSFKKEFWTKIKEQKKDSLS
                     FTSDFAGGIPLRERFGLTSTTPLVKFDKTKINIINKDSLAIDFTTEYDEFNQKFYIDF
                     KKEPLEKYTISAMPNAFTDFFENVNDTLNYKLTTKNTTDYGNLRLILENAKRFPIIIE
                     LTDKDGKVKATAYTEKETTIEFMNIEPLLYTVRIIYDDNKNKIWDAGSYLENRQSEEV
                     IYFPKEIDVRANWDVEQPINIGITN"
     gene            complement(18873..19640)
                     /locus_tag="PK218_00605"
     CDS             complement(18873..19640)
                     /locus_tag="PK218_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HPW97041.1"
                     /translation="MKIALLQTSIDWENPLENRSHLSQKITGFMEDVDLIVLPEMFSS
                     GFTMNPKAVAETMKGETISWLQHLAKAKNCAITGSLIIKEKGNFYNRLVFVFPDGKMQ
                     HYDKRHLFTLAGEDKIYTAGKEKLIVEYKGFKICPLVCYDLRFPVFARNVENYDVLLY
                     VANWPKVRTNAWDILLKARAVENMCYTIGVNRVGVDGNNHEYIGHSQAIDFLGNYIIE
                     PQETEGVFIVELDKKKLDETRNKLAFLDDKDEFKLSC"
     gene            complement(19685..>22450)
                     /locus_tag="PK218_00610"
     CDS             complement(19685..>22450)
                     /locus_tag="PK218_00610"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPW97042.1"
                     /translation="SQNYQQYVNPFIGTGGHGHTYPGATVPFGMVQLSPDTRIDGSWD
                     GCSGYHYTDNVIYGFSHTHLNGTGCSDFGDIMLMPTMGEPSFDPKEYSSIFSHANEKA
                     SAGFYSVKLDKHNIDVRLTTSTRVGFHEYTFNKAGQANIILDLNHRDKLLEGEVRIID
                     EKRIWVKRRSEAWARDQYVFALIEFEKPFGINKVKCNGEKGRRFNGTELALSFSRKVE
                     KGEKLKVKVTLSPTGYEGAELNASEIKDWDFNKVKKAAEQLWYKELSKIEISESNNDK
                     LTIFYTALYHTMVQPNIAQDIDGKYRGRDNKVHEGEGFDYYTVFSLWDTFRAAHPLYT
                     LIDKKRTADFINTFLKQYEQGGRLPVWELASNETDCMIGYHSVSVMADAMAKGIKGFD
                     YEKAFEAAKHSAMLDHLGLDAYKKQGFISIDDEHESVSKTLEYAYDDWCIAQMAQILN
                     KKEDYEYFMKRSQSWKNVFDWNIGFMRPKKNGGWDKPFDPREINNNYTEGNSWQYSFF
                     VPQDIPGMIDAYGGSEKFEAKLDEMFNSESKTTGREQVDVTGLIGQYAHGNEPSHHMA
                     YLYNYVGKPEKTTEKVHYILDNFYKNAPDGLIGNEDCGQMSAWYVLSSMGIYQVTPGR
                     LEWSTTKPNFKSIKVHLNDKVDYIDDERSGNYLSQFGLDEYLKKPHYEQNQPIIPVPV
                     IEANSKSFKNNLEVTISYDKLDFVRDQKHLKAYYRIIKDNNSEPFVEYSKPFLISESA
                     KIEAYLQPDFMEPSNTISATFFKKPNNYTIDIKSKYNPQYHAGGNDGLLDGIFGTTNW
                     RKGDWQGYQSQDFEAVVDLQEIKEISYLSSNFLQDSRSWILMPTKVDYYVSTDNINFT
                     KIGTIENTLDAKETENTIRDFSITLEKPIKARYVNVIAKNYGKLPEWHQGFGGDAFIF
                     IDEITVK"
     assembly_gap    22451..22550
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22551..23203)
                     /locus_tag="PK218_00615"
     CDS             complement(<22551..23203)
                     /locus_tag="PK218_00615"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HPW97043.1"
                     /translation="MATKKNINIKLKIWRQKNAKEKGKIVDYKLDNVSTDSSFLEMLD
                     QLNEQLVNERQEPVAFDHDCREGICGMCSLYINGQAHGPDSRTTTCQLHMRKFKDGET
                     IYIEPWRSKAFPVVKDLVVDRSSFDRIQQAGGFVSVNTSGNTIDANATPIPKDDADKA
                     FEAAACIGCGACVASCKNGSAMLFVGAKVSQYALLPQGKVEATQRVLNMVRQMDEEG"
     gene            complement(23210..25219)
                     /locus_tag="PK218_00620"
     CDS             complement(23210..25219)
                     /locus_tag="PK218_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HPW97044.1"
                     /translation="MKLDSKIPQGPLDKKWTDYKDHLKLVAPNNRPKLDVIVIGTGLA
                     GASAAASLGEMGYNVKAFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSTYRLFYDTIK
                     GGDYRAREANVHRLAEVSANIIDQCVAQGVPFARDYGGNLDNRSFGGVQVQRTFYAAG
                     QTGQQLLLGAYSALSRQIGLGRVKMFNRHEMLDLVKVDGKARGIIARNLVTGELERHS
                     AHAVIIASGGYGNVYFLSTNAMGSNVTASWKIHKQGALFANPCYVQIHPTCIPVHGTN
                     QSKLTLMSESLRNSGRIWVPKKKEDAEAIRAGKLKPTQIAEEDRDYYLERRYPAFGNL
                     VPRDVASRAAKERCDEGYGIEANDTNEGVYLDFSTEIRNKGKEVAYAKGNHNPSEEEI
                     IKLGKQWIEEKYGNLFEMYEKITDENPYETPMKIYPAVHYTMGGVWVDYNLQSTIPGC
                     FVAGEANFSDHGANRLGASALMQGLADGYFVLPYTVSNYLADEIRTGKISTQSAEFDE
                     AESRVKDSIDKFLNNNGSKSVDHFHKRLGLIMWNKVGMARNEKGLKEAIEEIAALKEE
                     FYKDVYVPGSADELNPELEKALRVADFIDLGQLMAMDALQRKESCGGHFREEYQDSEG
                     ETLRDDVNMAFVGAWEYMGFDITKENLHKEELKYEFIKIAARNYK"
     gene            complement(25236..26153)
                     /locus_tag="PK218_00625"
     CDS             complement(25236..26153)
                     /locus_tag="PK218_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPW97045.1"
                     /translation="MAKSALLKSSLAKKYWMALTGLFLCLFLTGHLAGNLQLLVPNNA
                     LNFNKYALFMTTNPAVKLLSYLTYISILFHAIDGFILTYQNVKARPIGYAKNTPSKNS
                     SFSSRNMAVLGTIILVFIITHMVNFWAKMHFDRNMPLVAKTTDNGMGQKQIQLVGTKD
                     SPIPFLNTNGEYVISENFIKTVKEQTQGKYDLQLVNNTTLVNKVDGEIVFEGYKDLYK
                     ITIAFFKSEAKFMGVIPKEGLVFTILYVLAMATLAFHLLHGFKSAFQSLGINNPKYNS
                     AIKGFGTFFAIVVPVLFAVIPVYIHFFLK"
     gene            complement(26314..27180)
                     /locus_tag="PK218_00630"
     CDS             complement(26314..27180)
                     /locus_tag="PK218_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97046.1"
                     /translation="MKKLLFITLSSLSLLLFSCSKDDSNSEPMLIIKFKFDPTQIRLN
                     NLGQPSVLAAGHAAQSPQFNTISAHYVEFAPNTTTQLGSGSIVYHAPETIIGGSNALD
                     FDQSKIVAEGETFLKIPLSQLNSGSYEWLRVSLSYQNYQINIRHQNVDYQGTLASFIG
                     FNTYIGSFSIGNNFFDVNGNRAQGFWAFALNNQAYSSSGQAPANATTVPNPLASTSPI
                     PAGSCVVTGKFPTNLVITGNETNDVVVTLNLSTNNSFEWQEVTSDGKFEPSIGENVVD
                     MGIRGLIPTYVK"
     gene            complement(27190..28035)
                     /locus_tag="PK218_00635"
     CDS             complement(27190..28035)
                     /locus_tag="PK218_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HPW97047.1"
                     /translation="MKSLISILFLVSFLNFAQNKSPYQLFDKNGKKVSYKKLLKNIEN
                     ADVLLFGEYHNNSIVHWLQLELTKDLAQKKQVILGAEMLEADNQKQVNQYLNDEINQK
                     QLDSTARLWNNYKTDYKPLVDYAKKRKLKFIATNIPRRYASMVYKNDFQALDNLTDEE
                     KLWIAPLPIPFDITLPNYQSMMEMTEHAGEKMPKAQAIKDATMAHFINKNLAKNALFI
                     HFNGTYHSDNFEGIYWYLKKLQENLTIKTISTVEQDDISKLDKENYTKADYIFVIDID
                     ATKTY"
     gene            28124..28720
                     /locus_tag="PK218_00640"
     CDS             28124..28720
                     /locus_tag="PK218_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPW97048.1"
                     /translation="MATTKPKEAFLLFVNQFIDKTDVAMQLQIKAALSSFSFSFLKKN
                     DFLVQENEICNHFCYIESGILQHAIEVDGEEKTTYLGLRNSVTSSLKSFLNQTASQKS
                     IKALVDTELWIIDLKNFQELLQNNAAFHHFYFKLIEKQIMLIDDYRIDLLTLSPEERY
                     KKLLVTEPKLLQEVPLHYLASFLGISNRHMSRIRKNIK"
     gene            28793..30085
                     /locus_tag="PK218_00645"
     CDS             28793..30085
                     /locus_tag="PK218_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPW97049.1"
                     /translation="MKYHPIDRNLFIKNRAKFTAGMKPNSVAVFNSNDIYPVSADSTL
                     PFAQHRDIFYLSGVDQEESILLLFPDAPYAHLKEILFLKETNEHIAIWEGEKLTKERA
                     YEVSGIKSVMWLQDFHKTLNEVMTYAETMYINTNEHYRASVETETREARFVKWWKENY
                     PAHKVEKSNPILQRIRSIKESEELDLIQKACDITEKGFRRVLNFVKPNVMEFEIEAEF
                     MHEFLRNRSKGFAYTPIIASGNNANVLHYIENNQQCNSGDLILLDVGAEYANYSSDMT
                     RMIPVSGKFTNRQKEVYNAVLRVKNEATKMLVPGNFWKQYHVEVGKIMTSELLGLGLL
                     DKADVQNENPDWPAYKKYFMHGTSHHMGLDTHDYGLLYEPMQANMVFTVEPGIYIPAE
                     GLGIRLEDDVVIQKTGEPFNLMRNIPIEAEEIESIMNS"
     gene            30239..31021
                     /locus_tag="PK218_00650"
     CDS             30239..31021
                     /locus_tag="PK218_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW97050.1"
                     /translation="MKTTIYKNTKISFTDQGKGTAVVFLHGFLENKTMWDAFIPDLVK
                     KNRIITIDLLGHGDSECLGYVHSMEDQADMVQAILHELKIRKAVFIGHSMGGYIALAF
                     AELYPENIKGLVLLNSTSRADSNERKVNRDRAIVAVKQNYTNFVRMSIANLFSEDNRK
                     KMIDDIEKVKLEALKTPLQGIVAALEGMKIRIDREVILHFAPFPIQLILGKKDGVLIY
                     EENLEQIEGTKVKLTTFPDGHMSHIENQFELKKVLLSFLKGI"
     gene            complement(31069..31452)
                     /locus_tag="PK218_00655"
     CDS             complement(31069..31452)
                     /locus_tag="PK218_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97051.1"
                     /translation="MKKIIILTFLILASCASKVKFPVSNVTPGAEITVKTSKDDNGNS
                     KLKLKAKFLTSPERLSPSKKFYLVWLQTKSNGLQNLGKFETDASSKASFETITPYEPD
                     EIFITAEDDAAIKYPTGQEISRAKL"
     gene            complement(31509..34262)
                     /locus_tag="PK218_00660"
     CDS             complement(31509..34262)
                     /locus_tag="PK218_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPW97052.1"
                     /translation="MSKTTFLKQLAQTIIKKHSENLSDVVIILPNRRAKVFLINELQL
                     ATDKTIFAPEITSIEDFIQTISGIRSIDSVELLFEFYEVYCKVEEQDKLDEFENFANW
                     AKTLLQDFNEIDRYLLDPNKILKYLENIKEIEHWSVDSNKKTNLIEKHLLFWKKLPLY
                     YHSFYKHLFNKGIGYQGLIYREAKENLKNYTDNLKNKIFIFAGFNALNQAEELIVQHF
                     LAIDRAEVFWDLDEVFLRDEFHDAGLFQRRFKKEWNYYKTNPYQWIAKDFATEKKIEI
                     ISTPKAIGQAKIVGNLIHQYAEKENLQNVAIVLGEENLLLPILYSLPSTVDALNVTMG
                     FSSKNNPAQLLIAKLFKLHVNTTNRNSSSYTMYFKDVLDVLTNPLIEPHVDAYDLVNK
                     INKNNFSFISWNKLHELYGKENALYKLLFQKWDANPVKFLESIGEILVHIKNKLNREN
                     EEEKITTAFVYSIYKVINKLISYFDHQNSVTDLKTVFAIYKQVIDVAEVSFEGEPLQG
                     LQIMGVLESRVLDFDTVIITSVNEGKFPAGKSINSFIPYDVKREYGLPTFKEKDAIYT
                     YHFYHLLQRAKNIYLLYNSDSDGFDAGEKSRFITQLEIEKQPNHTLKHSFYNPAVPEI
                     ANRPIIIEKSELVQQRLKEIAHNGFSPSSLTSYIRNPLQFYFQRILRIKETDEVEENI
                     AVNTLGTIIHATLEELYKPFLNRILTIDDIKNSFPKIANEVTLQFKKEYKEGEIKTGR
                     NLLAFEVAKRNVENFLNSELKSLENGDEIQVLSLETSYERTIEDKRLPFPVLIKGNID
                     RVELHNGKIRIIDYKTGKVEGRNMTLYDWNGLTADIKNDKIIQVLAYAFMYEPEAKNR
                     QIEAGIISFKNMKAGFLPFNFKVDKVTTEVITPEIMENYLNEIVNLLLTIFDRDKPFE
                     ELL"
     gene            complement(34327..35721)
                     /locus_tag="PK218_00665"
     CDS             complement(34327..35721)
                     /locus_tag="PK218_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW97053.1"
                     /translation="MKHLNKLFVALVMLAGISSQAQDSNNPWAISFGVNAVDTRVSAA
                     SKLEDQFSEFFNAKDNWNILPSVSFVNVSKYVGSNFSFGVTGSINKLTKFVNPRVGTN
                     DYTVTNPGDVNYYAVDGVINYSLMNVIKSKTIDPSIHVGGGYTWLGDVSSAGTVNGGL
                     GLAYWFSENVGLSYRATYKYSFEDSRADMPTHMQHFAGLTFKFGGKDTDGDGIYDKDD
                     ACPDQAGLKQFNGCPDTDADGIADKDDACPDVAGPVEFNGCPDTDGDGIADKDDACPD
                     VAGLKSLNGCPDTDGDGIADKSDKCPDVKGPKENGGCPWPDRDGDSVLDKDDKCPDVK
                     GTVANNGCPEVSDEVVKRLNDYAKTILFDSGKASFQQQTYPVLQSITGILKEYPNSKF
                     SLEGHTDSDGKDAANQKLSEDRAAAVKNYLVENGIDTSRLSSKGFGESMPIDTNKTKA
                     GKANNRRVEVKLVK"
     gene            complement(35853..37046)
                     /gene="kbl"
                     /locus_tag="PK218_00670"
     CDS             complement(35853..37046)
                     /gene="kbl"
                     /locus_tag="PK218_00670"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPW97054.1"
                     /translation="MYGKIQQHLQNELKAIEENGLYKKERIITSPQGAEIMVNGETVL
                     NFCANNYLGLSSHPEVIQAAKDALDSHGFGMSSVRFICGTQDIHKTLEQKIADFYGTE
                     DTILYAAAFDANGGVFEPLLGEEDCIISDSLNHASIIDGVRLCKAARYRYENSNMEDL
                     EMQLKKAVEAEHRFKLIVTDGVFSMDGLVAPLDKICDLADKYDAMVMVDECHAAGFIG
                     ATGKGTLEAKGVMGRVDIITGTLGKALGGAMGGYTTAKKEVIEILRQRSRPYLFSNSL
                     APAIVGASIKVFELLKKDTSLRDKLESNTNYFKEGMKNAGFDIIDGDSAIVPVMLYDA
                     KLSQVMADELLKKGIYVIGFFFPVVPKGKARIRVQLSAAHTKEHLDRAILAFTEVGKK
                     LNVIK"
     gene            complement(37082..40249)
                     /locus_tag="PK218_00675"
     CDS             complement(37082..40249)
                     /locus_tag="PK218_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPW97055.1"
                     /translation="MTKKPFSIYDASAGSGKTYTLVKEYLKIILLAKKPDAYRNILAI
                     TFTNKAVHEMKSRVVQNLSEFAKENPSDKAHQLMQVIHSETGLSLQTIHLKAKSIIKH
                     LIHNYAAFDISTIDKFTHKVIRSFAHDLNLPITFEVSLDTENLLTEAVDAIIAEAGND
                     KQLTELLVNYTLEKTNDDKSWDISREIMETGKLILNENNREEITHFHNKSIEDFVEIK
                     KKLVDLCEEILEETANLAAKALELIDSKGIDIKSFSAGHFPNHLKKLSEKEFNPKNKT
                     YREFEDIKINKTAKDKEIIENIIPDLIENLDKIYKKYEKLFFYEAFLKNITPLSLLNT
                     LNSKLKSIQEEQNILSIAEFNQLIYEQIQNQPAPFIYERLGEKYRHFFIDEFQDTSEM
                     QWQNLIPLIGNSLSGQDEEGTQGTLLIVGDPKQSIYRWRGGKAEQFIALSKDENPFPN
                     VEKNTESLKTNYRSYAKVIEFNNAFFGFLSNQFENEDYKDLYANHSHQKSNDKKGGFV
                     SISFIPKLDKNNFDEEENPSKDELYLEKTVESILNAKEKGFKNKEITILTRKKVQGTI
                     LANYLTEKGIPILSSESLLLGSSSEVKAIVDLLRYLNNYSDLEAKANFLYYLGRNATV
                     VKENKLPIHDFMAKGMEFSAENDFENWLTEFGITFSFKNIRKKSLYEATEIIISRIIA
                     IEKRTAYVQFFQDLVLEQDLKKQAGIGDFLKYWDANSHKMSIPSPEGNDAVKIMTIHK
                     SKGLEFPVVIFPFADEDYSKGPKEKIWLFADEEMGLPKVLVDKNSGVENYGEQASEVY
                     LHKKQEELLDDINVLYVALTRAEEQLYIISEMQTRSNSTGEYPKNMASFFINYLIQKG
                     EFEEGKMEYLSGENNKLSAPNTIEDKTQTIPQMAETLDPKNIKIAQRESLMWNTIQQK
                     AIEFGNVLHEILAFIETKADIDLALTKAIESGLIVPAQKGEVQKTLEQIVGHDELRSF
                     FATEHKILNEKTIIQKNGGIIKPDRMAVSKENEVFLLDYKTGQHLPKHKAQLDHYQIA
                     IENMGYKVCKKTLVYIGDKIEIVNL"
     gene            complement(40249..40767)
                     /locus_tag="PK218_00680"
     CDS             complement(40249..40767)
                     /locus_tag="PK218_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW97056.1"
                     /translation="MQLDWQPKNLQNELIQLIPLKENHFEELYEVANDELLWEQHPNK
                     LRYQRNVFQNFFDGAMVSKGAFLILDTKTNEVVGSSRFYDYDEINKTVLIGYTFIGRK
                     FWGKGYNLALKKIMIDYAFQFVEKIHFHIGATNFRSQKAIEKIGAKKIAELEVAYHGE
                     DSKLNFIYEILK"
     gene            40870..41478
                     /locus_tag="PK218_00685"
     CDS             40870..41478
                     /locus_tag="PK218_00685"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPW97057.1"
                     /translation="MSFELPQLPYAYDALEPHIDARTMEIHHSKHHNGYTTNLNAAVE
                     GTDLEGKTIENILINLDMKNAAVRNNGGGFYNHNLFWRVMSPDGGGKPTGELADAIDA
                     AFGSFDAFKVQFAKAGATRFGSGWAWLCVHKGGKVEVCSTPNQDNPLMPDVGCGGFPI
                     LGMDVWEHAYYLHYQNRRPDYIEAFFNVINWTEVARLYATEK"
     gene            complement(41911..43809)
                     /locus_tag="PK218_00690"
     CDS             complement(41911..43809)
                     /locus_tag="PK218_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPW97058.1"
                     /translation="MSDAIKHECGIALLRLKKPLEFYEQKYGSAFYAIQKMYLLMEKQ
                     HNRGQDGAGFASIKLDVEPGERYISRVRSNQAQPIQDIFAQINERINEEMSLHPEYAE
                     NISLQKAKIPYIGELFLGHVRYGTFGKNSIESVHPFLRQNNWMHRNLIVAGNFNMTNV
                     TELFNSLVELGQHPKELADTVTVMEKIGHFLDEDVTDLYHECKTNGLSKREASPVIAE
                     KLNIAKILKRASRGWDGGYAMAGLLGHGDAFVFRDPAGIRPAYFYEDDEIVVVASERP
                     VIQTVFNVEFEKVQELQPGHALIIKKNGIVTEEEIITPTIKKACSFERIYFSRGSDAE
                     IYQERKELGKLILPAVLEAIENDTDNSVFSYIPNTAETSFYGMVEAANDFLNQRKNQF
                     ILTNRKTLTKEKLEEILSVKIRTEKIAIKDAKLRTFITDDANRDDLVAHVYDVTYGVI
                     KHTDNLIIIDDSIVRGTTLKMSILKMMDRLRPKRIVVVSSAPQIRYPDCYGIDMAKLE
                     GLIAFQAAVELLKENNKTNIIEEVYKKCKLQENIKDSEVINHVTEIYAPFTDQQISDK
                     IATLLHSDDIKAEIKIIFQTVENLHRACPKNLGDWYFTGDYPTPGGNRVVNRAFINFY
                     EGNNARAY"
     gene            complement(43871..44797)
                     /locus_tag="PK218_00695"
     CDS             complement(43871..44797)
                     /locus_tag="PK218_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPW97059.1"
                     /translation="MNKLLIVGTVAFDAIETPFGKTDKILGGAGTFIGLSASNFSVNS
                     AIVSVVGDDFPQEYLDLLTERNIDISGIEIVKGGKTFFWSGKYHNDLNSRDTLITELN
                     VLADFKPVVPADFTNSDVVMLGNLHPLVQSSVLDQMTQAPKLVVLDTMNFWMDCALPE
                     LLDVMKRVDVITINDEEARQLSGEYSLVKAAAKIHTMGPKYVVIKKGEHGALLFHGND
                     VFFAPALPLEEVFDPTGAGDTFAGGFAGFITQSENISFNNMKNAIIHGSNLASFCVEK
                     FGTERMLVLTKEEVNDRLKQFKSLTQFDIELQ"
     gene            45062..46336
                     /locus_tag="PK218_00700"
     CDS             45062..46336
                     /locus_tag="PK218_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HPW97060.1"
                     /translation="MYALVDCNNFYASCERVFRPDLNGKPVVILSNNDGCVIARSNEA
                     KAVGIPMGAPEFKYRTLLKENNVTVFSSNYALYGDLSNRVMQILADFTPNVEIYSIDE
                     AFLNFDGMTIEDYHDYGLQMKNRIQKWVGIPTCVGFAPTKALSKIANRIAKKFVIRTN
                     GVYIIDTDESRIKALKWTKIEDVWGIGYRLSKKALLQNIKTAYDFTLPQHENWIRKQM
                     GVVGLRLKHELEGKSVLDLEPEKTQKKTIAITRSFPKQLNDFDALKERISTFATVCAE
                     KLRAQKSSCYTIIVMLVIDKHKYKTTKYYFNSAITLPFATHSSLTISNTAIRILKELY
                     AGNEHFTFKKAGVIVTELIPEHNLQINLFENENPKHHMLMKTIDNINRKMGEKKVKIA
                     NQNLNLTWDMKQNHLSNKFTTDFTKILEVLCH"
     gene            46327..46767
                     /gene="umuD"
                     /locus_tag="PK218_00705"
     CDS             46327..46767
                     /gene="umuD"
                     /locus_tag="PK218_00705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HPW97061.1"
                     /translation="MPLEKNQKLQFFIPDFESDIQIAYSHEMVSAGFPSPANDFLETK
                     IDLNKELSKNPLATFYVKVKGDSMINSGINDKDVLIVDRSLEPNDNKIAVCFIDGEFT
                     VKRIKLDKNCLYLMPENKNYQPIKITEDNQFVIWGIVTYVIKAV"
     gene            47003..48625
                     /gene="ade"
                     /locus_tag="PK218_00710"
     CDS             47003..48625
                     /gene="ade"
                     /locus_tag="PK218_00710"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPW97062.1"
                     /translation="MQIQGQIVDIENKRIYSGEIIVQNGKITSIIEKQHNNKKYILPG
                     FIDAHIHIESSMLVPSEFAKIAVLHGTVGTISDPHEIANVLGKEGVHYMIENGKKVPL
                     KFHFGAPSCVPATFFETAGAVIDSEQIKELMAMDDIYYLSEMMNYPGVLFDDEEVLKK
                     IAWAKHFNKPVDGHAPGLRGEPIKKYISAGISTDHECFTYDEAAEKLSLGMKVIIREG
                     SAAKNFEALIDLLPEHYENMMFCSDDKHPDDLIVGHINLLCARAVAKGFDTFKILQMA
                     CINPVKHYNMNIGLLKENDVADFIVVEDLKNFKVLQTYINGELVASNGQSFVKHIDFE
                     TPNNFNTSKKQVSEFTIPSNAATIRVIEALEGQLITNEIHCKPKIENGNLVSDVTNDI
                     LKMAVVNRYANTKPAVAFIKNFGLKKGAIASSVAHDCHNIVVVGTSDEEICNAINLII
                     ENTGGICAINGEEQKSLALPVAGIMSNKNGWETGKLYQEIDAMAKQLGSTLKAPFMTL
                     SFMALLVIPDLKLSDKGLFSGKSFAFTDLEVS"
     gene            complement(48686..49393)
                     /gene="rsmI"
                     /locus_tag="PK218_00715"
     CDS             complement(48686..49393)
                     /gene="rsmI"
                     /locus_tag="PK218_00715"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPW97063.1"
                     /translation="MGKLFIVPTPIGNLEDMTFRAIKVLKEADLILAEDTRTSGKLLK
                     HFEIATHMHSHHMHNEHKTVENLIKRLQAGENIALISDAGTPAISDPGFLLTRACVEN
                     NIAVECLPGATAFVPALVNSGLPNDRFVFEGFLPEKKGRQTRYLALAEETRTMIFYVS
                     PHKLLKTLAEYIQYFGAERPVSVSRELSKLHEETVRGTAEEVLNHFEAKPPKGEIVVV
                     VGGKVSEARAKNKEERE"
     gene            complement(49416..50294)
                     /locus_tag="PK218_00720"
     CDS             complement(49416..50294)
                     /locus_tag="PK218_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97064.1"
                     /translation="MKKRVEAELISIAHRILKLKNKSEIDQLYKETQKLYEALTILKF
                     YGDNYEMLKNSIPVEEIEEKVAAFIESNEVITPVAVAVKKEPEVDVLEETIIPTAKKI
                     EEEPVVVGEIIIEEEEEIEEEIEASAEPIVEETEEESFMPSFELSFEEEKEALEETID
                     KETPSKQVTMDDVFGDHFKEPVFVKPEDNVQGKFVKVFEEKNVEEAGRVLSLNDTLSN
                     SISIGLNDRIGFVKHLFNESNEDYNRVLSQLNTFDTFDEAKTFLNEMVIPDYNYWVGK
                     EEYLERFMEIVEKKFK"
     gene            50471..51091
                     /locus_tag="PK218_00725"
     CDS             50471..51091
                     /locus_tag="PK218_00725"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPW97065.1"
                     /translation="MFLENTVNHKEQFGWIEVICGSMFSGKTEELIRRLKRAQFAKQK
                     VEIFKPSIDTRYDEEMVVSHNKNEIRSTPVPAAANIRILAQGCDVVGIDEAQFFDDEI
                     VSVCNDLANSGIRVIVAGLDMDFKGNPFGPMPALMATAEYVTKVHAVCTRTGNLANYS
                     FRKNDNDKLVMLGETEEYEPLSRAAYFNAIRENMIVKDAEHLNDKD"
     gene            51104..53548
                     /locus_tag="PK218_00730"
     CDS             51104..53548
                     /locus_tag="PK218_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HPW97066.1"
                     /translation="MTIAIRNIISILKANWVAKNDVAVIDSISIDSRSLQNDSNTLFF
                     AISGQNHNGHEYIEDLISKNVEHFVVEYIPENLKDKANFLVVENTLEAFQKFATFYRS
                     QFEFPVIGITGSNGKTIVKEWLNFLLSPDYNIIRSPKSYNSQVGVPLSVLGINEKHNL
                     GIFEAGISMINEMEKLQKIIQPTIGILTNIGSAHDEGFSDVGEKIKEKLKLFSNVNVL
                     IVNKNKTISCFINPKTKQFTWSSDDKKADVFITTSKTENKTIIQCTYLEDTFPVEIPF
                     QDQASIENAIHCLVLMLYFGYNPTVINARMSELYPIEMRLKIKTGSNNCTIIDDSYSS
                     DFQSLKIALDFLEHQKQHKKKTIILSDIFQSGLTDEELYSQVSLLIISNKISRVIAIG
                     ETISKFKRKFINGIAFENTNEFIDAFDTLDFQNETILIKGARDFHFEQIVTLLEHKTH
                     ETVLEINLNAITHNLNFYKSKLNPSTKLMVMIKAFGYGSGDFEIAKLLEHHKLNYLGV
                     AFSDEGIALKNAGITIPIMVLNPESTSFDAIINHQLEPEIYSIKGLKAFIKIAELKKI
                     THYPIHIKIDTGMHRLGFQESDFPELIELLTNNELVKIKSILSHMATSDSIEYKDFSI
                     SQIQLFDKLSSELKAALKINPIRHILNTAGISNFPDAQFDMVRLGIGLYGISSDDDEQ
                     KYLQNVGTLKSLISQIHTIPKGDSVGYGRRFIAKRESKIATIPIGYADGISRHLGNNV
                     GYVLINNKKAPIIGSVCMDMLMVDATDVECKEGNTVIIFGEQPTVKEIAKLLNTIPYE
                     ILTSISQRVKRIFYRE"
     gene            53611..53988
                     /gene="mscL"
                     /locus_tag="PK218_00735"
     CDS             53611..53988
                     /gene="mscL"
                     /locus_tag="PK218_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPW97067.1"
                     /translation="MGMISEFKEFIAKGNAMDLAVGVIIGAAFGAIVNSLVSDVITPA
                     LLNPALKAAQVENLAELKTDGGILYGKFLAAVISFLVIAFVIFIMVKGLNSMKKKEEA
                     APAAPAGPTQEELLTQIRDLLKK"
     gene            54120..55109
                     /locus_tag="PK218_00740"
     CDS             54120..55109
                     /locus_tag="PK218_00740"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPW97068.1"
                     /translation="MKVAVVGATGMVGEVMLQVLAERNFPVTELIPVASEKSVGKEIE
                     FKGKKYTVVGLQTAVDMKADVALFSAGGETSLDWAPKFAAAGTTVIDNSSAWRMDPTK
                     KLVVPEINAHELTKEDKIIANPNCSTIQMVMVLAPLHKKYNIKRVIVSTYQSITGTGV
                     KAVQQLENEYAGVKGEMAYKYQIHRNAIPHCDSFEENGYTKEEMKLVRETQKIIGDKT
                     IAVTATAIRIPVVGGHSEAVNVEFSNDFDVNEVRTILHNTDGVTVQDNLDTFTYPMPL
                     YAQGKDDVFVGRIRRDESRENALNMWIVADNLRKGAATNTIQIAEYLVKNNLI"
     gene            55212..55565
                     /locus_tag="PK218_00745"
     CDS             55212..55565
                     /locus_tag="PK218_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97069.1"
                     /translation="MNRKLLLTNFIVVFAMAFALLFQSIHSVEHLHQIVTEKACVHSE
                     TNSKTNFTHEHHGLDKCSLCDFTFSHFTSPIKVTFDFYSYELNTPKVVSLYETIKPNF
                     QGSLFSHRGPPFFIV"
     gene            55624..57831
                     /locus_tag="PK218_00750"
     CDS             55624..57831
                     /locus_tag="PK218_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW97070.1"
                     /translation="MKFIYLYFIVGCTMMINAQNKISGTVTDSNNNPIPSATVSLPEI
                     HKETSTDKDGKFTLNNIPQGTFKLLVSFMGYETQTQTISLSGNEIQLSIVLTEAIKHM
                     DEVIVSNVFKRLQSQNVMKVELESIKSLQQKGTATLIEGLATIPGVSQVSTGTSIGKP
                     VIRGLSGNRVLVYSQGVRMENQQFGEEHGLGLNDSGVESVEVIKGPASLLYGSDALGG
                     VLYFNPEKFANPNDFKADVSQRFFSNTLGSNTSLGLKTSTDKWKFLVRGNYNTHSDYQ
                     IPDGDRVTNTRFNEKDLKAGIGYNNASFATVFRYNFNQLDLGIPEDGIAEQSTSKKTN
                     FPSQGVDSHILSLHNNVFFKNSKLDIDLGYIYNDRKEFEDSFTPILHMKLKTFNYTVN
                     YHLPRKGKFETIIGAQGMNQTNTNFGEELLIPDATTNDFGIYATSLYEWGKNVLQAGI
                     RYDNRKVASENHGTIGNEGYFEAIDKSFDSFNASLGYKTNFSENLTMRLNLASGFRAP
                     NLAELTSNGVHEGSNRYEIGNSNLDNEQNFQTDLNLEYKSTHFEWFVNGFYNHISNYI
                     FISPSGTQLDGYDVYNYEQSNAALFGGEVGVHFHPHPIDWLHLTSSFETVTGRKDNGD
                     DLPLIPANKWNNTLKAEFEQGNWLKDGFATLNIVYTLAQNNFSEFETKTNDYTLVNLG
                     LGGEITINKSKFNLNLNANNLLNKTYVSHLSRLKTDGIPNIGRNIMLGINFTI"
     gene            complement(57868..58668)
                     /locus_tag="PK218_00755"
     CDS             complement(57868..58668)
                     /locus_tag="PK218_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HPW97071.1"
                     /translation="MKAHEIDYHIYGEEMQYVEIELDPQEIVVAESGSFMMMENGIQM
                     QTIFGDGSREQQGIFDKLLSAGKRVLTGESLFMTAYINQERGKKKAYFASPYPGKIVP
                     IDLTKYGGKFICQKDSFLCTAKGVSIGIEFSKKLGRGLFGGEGFIMQKLEGDGMAFVH
                     SGGTLARRELAAGEVLKVDTGCIVGFTKDVDYDIEFIGGIKNSIFGGEGLFFATLRGP
                     GIVYVQSLPFSRLADRIIASAPRNGGNSREEGSLLGGLGRMIDGDNRF"
     gene            58939..59226
                     /locus_tag="PK218_00760"
     CDS             58939..59226
                     /locus_tag="PK218_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX protein"
                     /protein_id="HPW97072.1"
                     /translation="MDPPTGAAGDFMNSLGATGYIFPVVGVLEIIIGLLLLLRKWVAF
                     ALILLAPISINILLFHLFLDIPGLSFALLVVVINSILIYKHWEKYRPLFNN"
     gene            complement(59293..60435)
                     /locus_tag="PK218_00765"
     CDS             complement(59293..60435)
                     /locus_tag="PK218_00765"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HPW97073.1"
                     /translation="MKFNTKVIHGNQHHEKVTGAVMPPVFQTSTYVQTSPGKPIGDYE
                     YSRAANPTRQALEDALASIENGARGLAFSSGLAATDCLLRMFKAGDEIIAMDDLYGGT
                     YRLFTRLYKDSGIKFHFVDMNDLAKFQSLINENTKLVWVETPTNPLMKLADIQEIAKI
                     TKKHKLLFAVDNTFATPYLQKPLDLGADIVMHSATKYLGGHSDVIAGALVIKDKTLGD
                     ELHFKQFATGATLGPMDSFLVLRGIKTLHLRVQRHCENGMKVAEFLSNHPEVDNVFYP
                     GLPDHPFHDIAKKQMSGFGGMVSFTFKSGKMQDAISFLEKIKVFTLAESLGGVESLAN
                     HPTLMTHASIPEEKRLEVGITDDLVRLSVGVEDIDDLLADLEQAFR"
     gene            complement(60708..61163)
                     /locus_tag="PK218_00770"
     CDS             complement(60708..61163)
                     /locus_tag="PK218_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPW97074.1"
                     /translation="MKTTFEILLTSRKVLFNFLEHNSVDKLNTIPEGYSNNLIWNIGH
                     IIVVQQLLIYKLSGLPLMISDRMVAKYRKGTKPEEMVSQEEINEMKSLLFSTVEQTKL
                     DFSNKIFKTYSEFTSMTGYTMTNAKEALEFNNYHEGLHLGIMMQIKKFI"
     gene            61226..61579
                     /locus_tag="PK218_00775"
     CDS             61226..61579
                     /locus_tag="PK218_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HPW97075.1"
                     /translation="MTNKIYYLATCSTCRKIIKSLPNSDKLIFIDIRENPIKEKDLEE
                     LYQLSGSYESLFSKKAQLYKAMDLKNKNLTEQDYKKYLLEHYTFLSRPVFIIDDKIFI
                     GNGQKNILDVVKALS"
     gene            61579..62466
                     /locus_tag="PK218_00780"
     CDS             61579..62466
                     /locus_tag="PK218_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW97076.1"
                     /translation="MSKRTWALIAATIVSIIYGMSFTIAKDVMPLYIKPFGFILIRVF
                     GATLLFWAISFFGPKEKIDKKDFPRIIVAALFGVALNMLTFFKGLSYTSPISGAVIMV
                     TTPMIVLILSAIIMKERMLKRKVFGIVLGLIGTGFLILYGKSVGNATNASLGNLLVFI
                     NAVSYGFYLIIVKKLMDKYNAFTFVKWIYTFGLLMVIPFGYNEFQEIEWQSIPTIILW
                     DIVFVVVFTTFITYLFNLISMRELKPTTVAVFIYLQPLFATIFAISLGKDELNLIKIS
                     AAVLIFAGVYLVTQKKNVQ"
     gene            62554..63414
                     /locus_tag="PK218_00785"
     CDS             62554..63414
                     /locus_tag="PK218_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPW97077.1"
                     /translation="MIQSMTGFGKASLQLPTKKITVELKSLNSKGLDLNTRMPSVFRE
                     MELGLRNQLSQRLERGKIDFSLYIEVTGEETSSKINVPIVKGYINQMKAVIPTADETE
                     LMKMAVRMPDALKTERDEIDENEWKEIQKVIDEALENIAQFRKDEGVSLEKEFLHRIA
                     NIMTLMNNAVAYDVERVETVKTRLRTALDELKENVDENRFEQELIFYLEKYDITEEKV
                     RLENHLNYFIETLAGTEANGRKLGFITQEMGREINTMGSKSNHAEMQKLVVQMKDELE
                     KIKEQVLNVL"
     gene            63474..64046
                     /gene="gmk"
                     /locus_tag="PK218_00790"
     CDS             63474..64046
                     /gene="gmk"
                     /locus_tag="PK218_00790"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPW97078.1"
                     /translation="MNKGKLLVFSAPSGSGKTTIVRHLLKQEDLNLEFSISAATRKPR
                     GEEVNGKDYYFMSIPEFKKHIKADDFIEWEEVYRDNFYGTLKNEVERIWAKGKNVIFD
                     IDVAGGLRIKSKFPQETLAVFVKPPSVDELKRRLKERSTESDDKINMRIAKAHVELAT
                     APQFDTIIKNYDLEIAKQEAYKLVKEFVSS"
     gene            64314..64679
                     /locus_tag="PK218_00795"
     CDS             64314..64679
                     /locus_tag="PK218_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPW97079.1"
                     /translation="MSGVKNYKELLIWQKGIKLVVLVYKLTRNFPKEEVFALTSQVKR
                     ASVSVPSNIAEGFGRKTDKSFNHFLNISRGSLNELETQLIIAKELEFIVDFNLYDEVI
                     LMIEEEAKMINAFAKTIKD"
     gene            64777..65358
                     /gene="nadD"
                     /locus_tag="PK218_00800"
     CDS             64777..65358
                     /gene="nadD"
                     /locus_tag="PK218_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPW97080.1"
                     /translation="MKIGLYFGTFNPIHVGHLIIANHMAEHSDLDQIWMVVTPHNPHK
                     KKNTLLDDYQRLHMVHLATEDFVKIKPSDIEFKMPQPNYTVNTLAHLLEKYPEHEFSL
                     IMGEDNLNSLHKWKNYEYILQHHHIFVYPRMNSGEIDEQFVNHEKIHRVGAPVIEISS
                     TFIRENIKNKKNVSPMLPQKVWDYLDHNLFYTK"
     gene            65632..66627
                     /locus_tag="PK218_00805"
     CDS             65632..66627
                     /locus_tag="PK218_00805"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPW97081.1"
                     /translation="MNQNPKFAVIGGGSWATAIAKMLCVNLPEITWYMRNEASIDHIK
                     TQKHNPNYLSSVEFDTKKLKLTSDINEAVAYADYIIFAIPSAFLSAELAKLTESLKGK
                     VIFSAIKGIVPETFLIVGEHFNQKYDIPLENIGVITGPCHAEEVALERLSYLTIACGD
                     SAKAKVVANTLASNYIKTKISDDIIGTEYAAMLKNIYAIAAGIAHGLGYGDNFQSVIM
                     SNAIREMKKFIKKVHKMKRNINNSAYLGDLLVTGYSVFSRNRMFGNMIGKGYTVKSAM
                     MEMSMVAEGYYAVKSAYKLNQEYKAETPIIDAVYEVLYEGKDARATFKKLTDKLD"
     gene            66639..68510
                     /locus_tag="PK218_00810"
     CDS             66639..68510
                     /locus_tag="PK218_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW97082.1"
                     /translation="MNKIITIVCVFLVSQTNFAQDKNTAKADVLFETYQYVGAIEEYL
                     KLADTKNATSYVYAKLGDSYYNIFNPEEAAKWYAKAVEGKKVDAEVFFRYAQTLKSLG
                     KYKEANSQMDVFSKLLPSDSRAKEYKENPNYIPSLSNQTKMFDVAETTISYKNQSSFG
                     AVLTNDNELYFTSTKSSKRTDKSTDQPYLDIYKAQRNTNGKFLEAEAVSELNTPYHDG
                     PVAISSDGKTMFFARDGLSEGSYQKDKTGTLKVGQQGIYKATKVGDKWSNIEALPINS
                     VSYSVTHPSLSEDGRTLYFASDMPGGIGQTDIWKISISDNGYGKPENLGSKVNTAGKE
                     NFPFITADEVLYFGSMGRQGFGGLDVYKIDLKTNEDAVNIGKPVNSEKDDFSFSFNKK
                     ANIGFFSSNRNGFDAVFSATPICKTNAIIIVTNKKTGEIISNAEVTIIDNKGNSIETQ
                     KTAAKGIVQYNVECNTFYSLQISAANYETASIVLEKSNEKETTTKVQLAPEEVIITEK
                     EVILKSIYFDFDKSNITAQGAKELDKLVNVLKKYPEMVIFVKSHTDSKGKDDYNLTLS
                     EQRAQATVQYVISKGIEKERISGKGFGATEPKINCGKKCTDEQHAQNRRSEFLIVKK"
     gene            complement(68511..68918)
                     /locus_tag="PK218_00815"
     CDS             complement(68511..68918)
                     /locus_tag="PK218_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97083.1"
                     /translation="MKKDIHIPIVENVFLAAVQEWSDDFMEKVWYVYLVNDSDFDLDS
                     VMVVSKAFGTVDGEMKQTSFLRHAFTQIPKVSVAKIEMIEKSVLRLNNEFMVTYFIGN
                     TLYDKKFIFKAQSISPDYVEEVPILFVDGVIVR"
     gene            complement(68994..>69646)
                     /gene="pheS"
                     /locus_tag="PK218_00820"
     CDS             complement(68994..>69646)
                     /gene="pheS"
                     /locus_tag="PK218_00820"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPW97084.1"
                     /translation="SEGPEIEDDWHNFTALNLPEYHPARDMQDTFFIQTNPDVLLRTH
                     TSSVQVRYMENNKPPIRTISPGRVFRNEAVSSRSHCIFHQVEGLYIDKDVSFADLKQT
                     LLYFTKEMFGKSKIRLRPSYFPFTEPSAEVDIYWGLKTETDYRITKGTGWLEIMGCGM
                     VDPNVLKNCGINPDEYTGFAFGMGIERIAMLLYQIGDIRMFYENDVRFLEQFKSSI"
BASE COUNT        22797 a        11575 c        11082 g        24092 t
ORIGIN      
        1 attgttgttg ctaatttttt cataatttga gactttttat ttttagttag ataacattct
       61 gcttctgtga gtgccgataa tgatttgagg caattgcaca cattcacgag gcaatattac
      121 aacagctctc attctgaagc cttatgaaat caggtgttta tggtagatgt atatattctg
      181 atagtgtatg atactcaaat cttggtgaat gaaatttaat ttttttgcga ttttgagttt
      241 tttttgggcg ttaaagcaat aaaagaatcg ttttaagaca tgatttctta ttcaacacga
      301 tggaaggtgt ttgaaaaata gcttcattta aatgagtttt aatgaagttg ttgaaaaaaa
      361 ataggttgaa attatcacct atttaatgtg taaattatta tgttattgtt atttcatttc
      421 cgaaacttct gtatagaaat tgcattaaat ttgaattcgt ttatgttaga aaaatgaaat
      481 agattagtgt aaatatgttg ctatgtctgt caagcatagt tatcgattct cctttgttga
      541 aatgacatat acgtgcaaaa tattattttg cccaacggaa taaaatgcat taaatttggt
      601 ttagtaaaaa acaaaaatag tttatacagg aaccgaaata ttaatggagg tttgatggtt
      661 ttgaaaagaa ttgattgtta tttttccacc cagagcatgc actctggttt tcatgttttg
      721 aatacctata ccgtttgatg atttttgagt gtcaaaccca acaccgttgt cgtttatgga
      781 taagcaaaga ttattttcat caaggatgaa agctataatt attttgttgg cattggcgtg
      841 tttattacta ttatgacatg cttcttggat aatacgaaac aaattcattt taatttcact
      901 tgaaattgca ttccacacaa tggcttcgtc aagttctaac aaaaaatgtg tatcagtagt
      961 ttcgttttgg acagccacaa aatcttggag caacgacaca aaactgtttt cttgttgaaa
     1021 aacttcattg ttaagattgt gtgcaatgac cctaatttct tgctctatgg cgtaaatgtc
     1081 tgaaatatgc gtcaaacatt tagtaatcgt tgtctcatct gatttttcgg caagtattga
     1141 aaggtttaat cgagtgcttg ccagcttgtt catgataccg tcatgtaggt ctagtgcaat
     1201 tcgttttttt tctgcttctt tggcagcttc attgctgttt ttttgcgaaa gcatcaattg
     1261 ataaatttct tcattggctt tttgttgacg ttgttccaat tccattttta gtcgattgct
     1321 gcgttgtttg ataataataa aaaacaatat cccaacaaaa aatagtgcta ccgataaccc
     1381 agccatgatg aattgatttt tttcggctat ttctttttgt tgtgagattt cgtcggtttc
     1441 taactcaatc ttagcaaaaa gatctttcga ttttcgattt gatagttgaa tactatcata
     1501 aatgctaatg tattcctcta aattagcttc tgttttttta ttatcagcaa acatatttga
     1561 ttttagtgct agcagtaaat ttgaatatat ttttgatttt tttgcttcgt ttaaagccaa
     1621 ttcagaataa attatggctc gatttctatt tccattccta atataatatt cagaaagatg
     1681 aatatagata tttattaaaa taggtttgta attaatttta tttattacac ttgatgcttt
     1741 tttgtagcaa tttaatgcct ttagataatc attttcattg aaataagagt atcccaaatt
     1801 gtcatttagc aatgcatata attcagggtc tttatttatt aaagttttat tttctaatcc
     1861 tattttaaat tttttaattg catttttata atctttcttt tttaacaata catttccaat
     1921 attattaaag caggaagagt atagaatatc atttgagtca attttacttt tctctacaat
     1981 tttcaatgca tctatgtgaa attttaatga tttatcatat tctcctaact cactatataa
     2041 tactccaaat tgatttagta tttgatagta atatttttta tcattagttt tttttatgta
     2101 gaacaaagat tttgaaagtg ttaattcgca tgcattataa tcacctatgt catatagaat
     2161 taggctttta ttcaatagca caagtgcgta ttcttttggt ttatttaatg ctttatatac
     2221 tttttcagct ttaatatata atagtaaaga gctatctaat ttattattta tttgtaaatg
     2281 gaatgctttc atacgtaaag catttccata atttacagaa tctttagttt caaacgactt
     2341 atcaagtaaa tatttataag ttttttcaaa acctttaaga ttattaagat gataattatt
     2401 aaatccaatt tttgataaat attccctatt tcgaaaagta tttttttgat ttatagctat
     2461 ttcaaacgcc aaatcattat acttcactct atccttataa tcaatagtat catttgctgc
     2521 taagtcaata tatttttgaa tagagtcttg tttagaatct gaagacgcat tttttttctg
     2581 ttcgcaagta atacaaacca aagccaaaaa aaggagtagt atttttttca taattaaccg
     2641 ttataaacaa attgagctcc agccgaagcc ggaacccaat ttagtaaatt tctattattt
     2701 taaatcatct accacgatta ttcatcgcgt accgtgatga ttacctcttc ttcaaacttg
     2761 tattcgcctt tttttagaat ctctttattg gctaatacaa tttcgtaagt ataaatgcct
     2821 tttggtaatt caaaaacaca ttcttttccg tctttaggaa caacgagttc ttttttaacc
     2881 tcgtctcctt cctcgttttt gcccacaatc ctaaacgtaa ttctatcggt gcgtttgttt
     2941 ttcacacaaa cactttcaat aagtttactg tcttttttcc catcgctttt ggcaacttta
     3001 aaaatactaa taccaccaag cagcaactcg ccacctttaa gcactttatc aagatcagat
     3061 tgggcaaaac tcatcattgg caagagtagt acaattgtta ggagtctttt gaatttcata
     3121 ttatgaaaaa attattatcc tattaaataa tcaaagagat ttatttttct ttttttctga
     3181 aagatatcca agatatgaaa aaataaaagc tacaataatc atcagtatat ttattcctag
     3241 taataaaagc aaagatttat ttttagggtt aaaaattaat tcatgtaaag taccaagaat
     3301 agctgctgct ataatataat aaataaatgt gcttctaaaa acgctcttca tattttttaa
     3361 ttttaattat aattaacctc caaataaact taaaaatgcg caacccaagc ctgcaacaac
     3421 agaagcatac atcccattgg cgcatccaac tgctaaagca cctgttacag ttcctacacc
     3481 tggtaaaaat gctgttcctc ccatagctcc ccaaagtgct ccaccacaaa aaccagcaca
     3541 atccgcagct cctatagctc tgccagctct gacagcacct cctcgactgg ctgaagttga
     3601 agaatcatta aaaagtaaat gccacttaga ataattctct tcccaataat tgaatgaagc
     3661 ttttcctgta tttaatgtac ttaaagctat ttctagatct ttatcttcaa actttttgtt
     3721 tgcttcaatt attttattgt caaaataact ttttgatttt tcgaattcta ataccttgca
     3781 atcattaatg gtatttatta aaaatagttt taattcttcg tttttatagt ttgctgactg
     3841 aatcttatta atagataagt ctgctatttc gatacttgtc tttccaaaac aattatcaaa
     3901 cccttctaat gattcattta ctattgacgg attttcatta ccatataccg aaattaatac
     3961 ttctttaaat aatagcttaa aatcggattg agtaattttt tctttccctt ctgtttcagt
     4021 aaatgttttt agtttttcat aatacttttc catttgaaca ttatggaaat caccaattgt
     4081 atttacatta atagattgtg aaaatgaaat cgaagtaaaa caaactgtgg caattatgcc
     4141 aaaaaataaa tttttcataa ttttgtaaat aattttaaat tattgctgtc cagctttgtg
     4201 tcgtcgaaaa catataaaag tttgggcagc tttttttttg gttggtttag tcgtattcct
     4261 tgtataataa caaacctcta aaataaacaa ggattttctg ttttattcgt cccgcaaaaa
     4321 attagaaaat cattttttgt ttcgtgtcac ttagttggtg gtaagccatt tttttaccgt
     4381 tttagtttaa aaaattgttt tgttattttg atattgtaaa tgtataaagg taatttcaaa
     4441 ctcaggtaca gtttttctgt acatttttag aaaagtgaaa aatagttttt cacacaaaca
     4501 ctttcaataa gtttactgtc ttttttcccg tcgcttttgg ctgctttaaa aatactaata
     4561 ccaccaagca gcaattcgcc acctttaagc actttatcaa gatcagattg ggcaaaactc
     4621 attattggca ataggaatgc taatgctaag agttttttga gttccatttt tcttttttta
     4681 actgaaaaat gttatttcta gattttaata tttatttttt tttgttttta aagcataaaa
     4741 gattgtcata aataaaaaca gagttccaaa acctaaaaga atcttagaga taaggcgata
     4801 ttcaaaagtc ataatgaata aataaccacc aataatacta aaacctatcg ataggattgc
     4861 gaaagtctta acatttttca taattttcat atttataaat taattagggt atgctgccgg
     4921 gcatggttta ccccaacgtg tacatgtata acacaatcca gcagccaaag cttgtacttg
     4981 aggattcgca ttccaataaa tccatccaac cagagaatca gtaaaatcat tttcaacagc
     5041 atccatacaa tttccaaacg cactacgatt ttgtacatta ggtgtaattt tagtaattcc
     5101 attttcattt acaccagtat atattagaac tctttcttga gaaaacgtaa atgttttttc
     5161 tttatctaat ttcaaaaaat aaaagacttg gtctggtatt tctttaaata aaaacacgta
     5221 taaaatttca aattcgttat caataatcgg cattaaaatt cccttactgc cgtttttaaa
     5281 cgtttgaatt tgaatattct tttcatcaaa tttaatatca gaattatctt tattgaaatt
     5341 ggacttttct aaaaactctt taattattac aaatatacct atgtttaatt ttgcttaagc
     5401 aatatctact ttggcagcta tcttattcga ttttgtcatt ttatatgatt ttataatagt
     5461 ttacttaaat taaaaaataa aagcagtttt ggcgaagcta aaagctgctt ttatttcggt
     5521 aatgcttaat taaagttaaa tttaacgctt gcaaatattt gtgacggtct tatgacaata
     5581 atattagaag tataacccaa gtcatttaat atcacttctt cgtatgtttt agtatttaaa
     5641 atatttgtcc attttaattc aaaatcaatt tttctttttt ccatagtata ttggtagctt
     5701 aaatctataa actggtttct gaaattttgg ttgttaaatt ggtaaatgta atcatctcga
     5761 taaattccaa atgtttgatt tgaaacagga tataataata tttttaagtt actttttaat
     5821 gtatttgttt tattaatatt tcctctttga ttaattgagt aaaagagatt atagtcaaaa
     5881 gttaaccaat tataataatt gttattcgct ttaaaagtaa aagtatttcc agtagaatta
     5941 attaatagtt tgttattatt taaaattata ttatttgaac tagaacttat tccatattct
     6001 aatgatactt ttgatttaat ttttggaatg tatttactta tttctatgtt tgcgtttcta
     6061 ttaattgatt tgttttttga agagatttgt tgaaaattag tttgaccgtt atccataaca
     6121 ttctgtgaaa ttgtattgtt agatgaatct tgacttaatt gataatttaa gtttaaaaat
     6181 aaattatata atatcaactt gtattcaaaa ttaccaccta tcttttgtga aattgattgt
     6241 tgaatatcag aattttgatt agtaaagttt aaagctgaaa aaatatatga tgggtaaatt
     6301 gaatttaaag tgccaaaatt attatttatt gagccatata atgtgtttga caattcagtt
     6361 gtaaatttat attttgtact aaaatttggt tcgaaagtta atttatttaa gtttctgtga
     6421 aaatttaagt tattatcttt tgcatttaaa gaataatatt ttaacggtaa attagtatta
     6481 aaagtcaatg attctccaac ataattaatt gaaaaacttg aggaagaaat atgttttttc
     6541 caaaccatat tattagaaaa gttatttcca aaactaatcg tttcattgct tgaattctcc
     6601 ccattaaaat tggtctttaa atttttagct tctatttcaa aaccaataga gggaattatt
     6661 gaaagttttt ttattgaaaa aatataagaa gcttcattag tcatactaat agttttatct
     6721 ataacatttt gattaacatt tttagcattt atatctgaga attgagggat attaatgtta
     6781 tttaacggct ctacaaaata gttttgttta tcatttatgt aattaaatgt agatttaaaa
     6841 ttaactaatt tttttccaac taaaaatata gcacttaatg aattttgaaa tgaataacca
     6901 ggtgaagaaa catattgatt agtatttgat gaattaatta aagtactgcc ttgaattgta
     6961 ttccaatttc ctttgtaagt tgtagtgttt tttaaaaaac ttttgtctga atttttagta
     7021 aatataatct gcgatttaaa ctgattattt tcagtcgaag aattattaag tctattatat
     7081 tcaattgtat tttgtaggtt tccaaaagca tcaaatatat ttacttttga atactgatta
     7141 ccattgttat ttattttatt attataaaag tttgtattag ctttaagctc ccagtttttg
     7201 gacacatcag ttaataaatt agctgaaaaa agttgagttt tattaaataa atatctgttt
     7261 tcatcaattt ttggtagttc ggattgtgat acatttaacc aattacctgt ttggttttcg
     7321 aatgttaatc cttcaaaacc ttcatcaaaa gataaagttt gtaactcata ggtaacatct
     7381 tcacctatat tatttgattt atagtttaat aggtattgat atttttttgt aaacaccatt
     7441 ggcgttaatt ttgcactcca taaaaaagga gataaaccaa caccagtatc tgctcgtcca
     7501 gttagagtta catctttttt tagtttaata ttaattgctg cccgttcact tgggacagaa
     7561 ttttttagag cttttattgg ttgatgattt tctaaaactt gtagtttagt taccgcatct
     7621 tttggcatag aattggttaa agaaccatat ccacctgcca ttaaatcttt accttcaaca
     7681 taaaatttat taatagccgt gccttgatat tttattaatc catttgcatc tacctcaatt
     7741 ccgggtattt tttttaaaac atctgataaa gttctatctt ctttaccttc aaaggatttt
     7801 acatcataag ttagagtatc tcctcttttt tgtatttctt ttatatcaat ttttacctcc
     7861 tctaattccg ttattttatt ttttaatgta aaatttaata cttgtgactt tgaatttatt
     7921 gattgtataa ttttttcata gttaattgcg tttacaacta ggtttaattt taagttagta
     7981 ctttcaatat ctattctata atctccattt ttatcagtta ttgaaaacgc taatacacta
     8041 ctatcagaag gatcttcaat agtaatactt gcattttcaa ttatttcttt atcttgatta
     8101 ataacttttc ctcttattat attttgcgaa tatgaaattg atactaataa taaaaacaaa
     8161 gcatttaaga tttttttcat ttaatatatt tattgatttt taatctgtta gttcaattgg
     8221 attataattt ttttccttat caatactata tttatcaatt tttttattta tattaggata
     8281 gccttcaatt tttattgttg acggttttac agatgcaact tttcttgctt tttcatattg
     8341 ttttttggtt actttttttg cctttggata attatgcatt aaaacattgt tagctactct
     8401 gtttttaaat ttgtaaagtg taaaagtata atcttttgca tcatcatata cttcaaaaat
     8461 taaacctggt aaaccaaaaa atttataagg accaaatggc aatgggtgct ttggtgaata
     8521 aaaagcaatc caatttctac cccatttagt tgtggtagct tttatgcatt ttattccatt
     8581 aatagttttt gattcttttg ttattttcca ttcaaaatcg attgtttcgg tatattgata
     8641 ttcgcctgaa aatggaattt cattagaaaa agaaatagta ttctcttttt tgtaaatatt
     8701 gtaaggtaaa tcagggacgt attttgcaaa aacagggaaa tctttttgag agtctccagt
     8761 tttttttatt ttaccattat cgacaagaga atctctaaca taaagactca tgttcttaag
     8821 aaaactttct ttctcattaa ataataggat aaatgtattt tttaaaaatt caggtttttg
     8881 gtttttatat tctttaaaag ttaattcgta acgtgcttca tttgtaatgt ttattttttg
     8941 ctgtccaaaa gtgaataagc aagtaaataa aatataataa tatttaaaca taataatttg
     9001 tttttaagat tttataaaaa aaagcaaaaa ggaagtaatt tcctttttgc taaaatattt
     9061 tattaataga aagttattgc tacattttct gtaccacaaa aaattccatt aattttttgc
     9121 agagatttaa caacactgct ttctccatca gtgcctaaaa catgcactgt aataccacaa
     9181 ctagtatgga aataacctgt ccaagcaaaa gtattagctg aaaataaaaa aacagctaat
     9241 aataaaaata cttttttcat aacttaaata ttaataaaat gttattgtta ctttacttgt
     9301 tccacaaatt tgtagattcg cttgctgtaa acacgcaaca tactccgctt gtgtattacc
     9361 atagcaaact acatgataag aacctccaca tgaagggtga aatgttccct cccattgtgc
     9421 aaaagttgta gctgaaatca taaatacagc tagaaataaa aatacttttt tcatgatatt
     9481 ataatccttc cctttatatt atgtgtttac cacaggacga taactttagg tactatttaa
     9541 attggttaat gtaccgccct ttttaataaa tattaatttt atgtactatc tttcgtattt
     9601 ccttgaatga attcaagtgt atcttcaagt gagtcgtctg tttagcacga tttcaaaatt
     9661 ttctgccaac tgttatttct atatttgttt ttccaaaaca attatcaaac tcttctaacg
     9721 attcattgac aattgacgga ttatcatcac ggtaaacaga aattaaaaca tctttaagta
     9781 ataagataca atcgaattgg gtaattttat cttttccttt tgcttcaata agtttttttt
     9841 aatttttcat aatgtttttc catttgtaca ttatgaaaat caccgatggt atttatattt
     9901 atagattgtg aaaatgaaat agaagtaaat caaaatgtgg caattaagcc aaataataaa
     9961 tttttcataa ttttgtaaat aattttaaat tattgctgtc cagctttgtg tcgtcgaaaa
    10021 catataaaag gttgggcagc tttttttggt tggtttagtc gtattccttg tttaataaca
    10081 aacctctaaa ataaacaagg aatttctatt ttattcttcc aacaaaaaat tagaaaagca
    10141 ttttttgttt cgtgtcactt agttggtggt cagccatttt tttaccgctt cttattgatt
    10201 aattactgtt ggtttatttt gttattgtaa aattacagaa gtgatttcaa actcaggtac
    10261 agtttttctg tacattttca tcaaaatgaa ataaagtttt ttacacaaat gctttcgata
    10321 atcttactgt cttttttccc gtcgcttttg gcaactttaa aaatactaat accaccaagc
    10381 aacaactcgc cacctttaag tactttatca agatcagatt gggcgaagga atattggaca
    10441 aatgataata aaataattaa taattttatt tttttcatac gttggaagac ataattaatt
    10501 aatataaatt gtaaatttgc aaaaacaata aagactatag caaaattaat ttttccataa
    10561 agatttatat attctttaaa ttaatttcat ccacttttca ttttattata aacaccataa
    10621 cttattaaaa atattaattg gtgtctagat atgatactat aaagagaatg aaattactct
    10681 tgcctattta acaattattt ttgctcatat cttttaatga attctttttt cattctttca
    10741 taaacattat aaatagcatc gtcttttagt agttcaatta agcaatattt gtatgaatcc
    10801 ttgccttcaa gaataatgtc tcctttaaat tctgtagtag ggcaatttgt tgcagtttca
    10861 attgacattc tattttgagg gcatccacta ccaacattta cattctgatt agccattaaa
    10921 actttaccat cctcagtatt aaaataaact ctaacattaa cagtccaaca acctgagcca
    10981 attgattcaa caaaatctga ctcaactgta atataaaagc taaacattct tgaacttgtt
    11041 tcaaacggtt cagatttaaa agaattgttt tccacaaaaa tttttggttc cccaatagat
    11101 tcaggtttca tactgttttg actaaaacac acattgctaa acactaacaa agtaataaga
    11161 cttaaaatta attttttcat atatttgtaa ataaataaga attattgctg tccaacttta
    11221 tgttctcgaa aacatataaa aggttgggca gctttttttg gttggtttag tcgtattcct
    11281 tgtttaataa caaacctcta aaataaacaa ggaatttcta ttttattctt ccaacaaaaa
    11341 attagaaaag cattttttgt ttcgtgtcac ttagttggtg gtcagccatt tttttaccgc
    11401 ttcttattga ttaattactg ttggtttatt ttgttattgt aaaattacag aagtgatttc
    11461 aaactcaggt acagtttttc tgtacatttt cagaaaagtg aaaaaaagtt gtttttacat
    11521 taaaaacaat atcctttgag ttttgaagtt tttattgtaa taattatact gtaaccctga
    11581 gcacaatcga aggatataaa taacttcgac tgcactcagt ctgacaatta aaacctaaat
    11641 tctaacgttt actaatataa ctagtctagt ttagatcgtt ttcaaaacag ccatcaacat
    11701 ttatgttttt tacataaata attataaaat gaatgacatt attttaagtt ataacagcaa
    11761 catttattat gtttcaatca atagtgctaa aactatcatc tgcaatagta aatgaaacgc
    11821 caaagtttat aactcttgta gttgtaattt taaggttact gccattatat taataatacc
    11881 ttaaatcaat cctttttttc ttgcagcttt taggatgtct tcgtcattgc ctttatctat
    11941 gccaagtatt gttttaatta cagcttttct tttgtcaacc gcactttgag aaagatgtaa
    12001 taaatcgggt aaccttttgg tttttacgcc ttgattaaga agtgctagga tttgttgatt
    12061 gtaagtgtca aattctaaat tagacttttc attccaagtt ttttgaaatg atactattgt
    12121 ttgagtaaaa tatgttttct cttttacaat ggcatcaaaa ccactaatca attctttggc
    12181 ttgaaaatca cttttcacga aaataccttc aggatgatat tctttcgtaa ttctttgtaa
    12241 aataatggat tccgaatgag aagtaaggat aattattttg caagaaggat ggtgtttttt
    12301 aatcaatgga attaaatctt cacccgaagc aatatttttt tctaaataag aaggtattgt
    12361 tagatcgatt aaagcaacat caaatctttt ttgtgtagta gtaataattt caaaagcagc
    12421 ttgtccagta aaagcttctg caacttccaa ttgataatct ttgattccaa aaacgagtat
    12481 agctttatat ccttcaataa ttgttggatg atcatcaatc attaaaacct ctacaatcat
    12541 aaagtaattt tttgtgaagc tatcaaatat tcactaaatc tccaaataat taccagaagc
    12601 aatacgatga ataatcaatt ctacattctc tttatacgat tttgagaatg gaagtttgat
    12661 ggtagagttt ttaatgtagc aaacattatt gcctgtatgg attcgggcaa cttgacttac
    12721 gtttaagata taactattat gaatgcgtaa aaactgtgtt ggtggtaata ctgattcaaa
    12781 agcttttaat gttttgaaag ctgttaccat ttcaccgtct tttaaatgaa tatctacaga
    12841 attattatcg gcttcaaaat aaataatatc tcttgaatcg atataacgat aatcgccata
    12901 cgatttgaca catattatta acggatcttc ttgggcaacg ataacttttt gacctggaag
    12961 tgttgttata gcctcatcat cgttggtttc gtcatctgaa acagcgtctt ctatggtttt
    13021 agcttcaagc ggttttgtgt tttctacaac ttcttctcta gaaataggct cttctggagt
    13081 ttcggtttct atagtttccg attcttcaat ggtttctttt gttgagtttt cttcttgggt
    13141 taaaacttcg tttgcaattt ctgtttcttc ttctgaagtt gctatttcta aagcttcatc
    13201 gttgctttct tcagaagctt cattttcgat ggaatctttg gtttcctcta aagtttcttc
    13261 ttccttttct ggagtgtttg tctcttcgtc tgtaacttta tcctcaataa catcttcaac
    13321 tacactttca tttgcaccgt ttgcttcact ttcaataatt ccagtttcaa aaactgctgc
    13381 atcaactata atttcagcag catcaatagg ttcttctttt ttgacttcta cttcttcaac
    13441 aattggtttt tcaacgggtt tttgaatggg ttcttgtcgt aaaaaatcgg aaaaagttag
    13501 tgcttgttgt ggtacggttt gttgctctac aacagaattg gaaacggtat tttgtgaaac
    13561 caatttgtcg aatttaaaaa tagcttttct aaatgcgttt aattctaatg gtttcatcaa
    13621 ataatccgct acaccatatt ccattgctct aaaaaccaaa ctttggtctt tggaagtcac
    13681 aataattttt gggacaatgg tcaaatagcg atacaattca ttgattaagt tcaacgaaag
    13741 attgctttct ttttttagag gattaatttc taagaagatt aaacttggct gatgttctaa
    13801 aattaaattt aatgctgctt cataattggt agcataaccc atataagcca aattttgaaa
    13861 acgcatagct gtagcttgcg tattcaagat gctttcgtga ttatcatcaa caattatgta
    13921 actgtgcttc attaacgttt ttccaaatta ttttagaaaa cattaatcgg ttttgttggg
    13981 tagattttgg ggaaatctgc tttgattttt tcaaatcagt cgtaaagatg ctttattgat
    14041 acaattctag aaaataaaaa cgatgattaa cccaaataaa cgttgaagtt atttaataat
    14101 ttattaacaa ttaaattttg gttatttttt ggttattata acaaaaaaac ttccttaaaa
    14161 aaggaagttt tgaatatgtt atttttcaaa tatttacatt ttcatcaacc agtttcgtat
    14221 atcgacttcg ctggcaataa tgtttctcaa ttcgctaatt cttactcggt cttgtttcat
    14281 ggaatctcta tgacgaatgg ttactgtttc gtcttccaaa gtttgatggt caacagtaat
    14341 acaaaatggt gttcctaacg catcttgacg gcggtatctt cttccaacgg cgtctttttc
    14401 gtcgtaggct acattgaagt cccattttaa gtcttcgatg atattttttg caacttctgg
    14461 taagccgtct tttttcacta atggtaaaac ggctgctttt gttggtgtta aaaccgatgg
    14521 taaacgcaaa actgttcttg tgcttccatc ttctaaagtt tcttcttgta aagctgttgc
    14581 aaaaactgcc aaaaacattc tgtctaagcc aactgaagtt tctactacat aaggaacata
    14641 gttagaattg gtttcgttgt caaagtattg tagtttttta cccgagaatt gttcgtgtgc
    14701 ttttaaatcg aaatcagttc ttgagtggat tccttctagt tctttgaaac cgaatgggaa
    14761 gttgaattcg atatctgcag ctgcgttagc gtaatgtgct agtttttcgt ggtcgtggaa
    14821 acggtagttt tcttttccta aacctaatga taggtgccag tttagtctag tttctttcca
    14881 gtattcgtac catttcattt cttcgcctgg ttttacaaag aattgcattt ccatttgttc
    14941 gaattcgcgc atgcggaaaa tgaattggcg tgctacgatt tcgttacgga atgcttttcc
    15001 ggtttgtgca attccgaaag gaattttcat tcttccggtt ttttgaacgt ttaagaaatt
    15061 gacaaatata ccttgtgcgg tttctgggcg taaatataaa tccattgccg attcagcaga
    15121 tgcgcctagt tttgttccga acattaggtt gaattgcttc acgtctgtcc aatttcttga
    15181 tcctgaatct gggcaagcaa tttctaattc ttcaattagt gcttttacat cttctagatt
    15241 tccgtcgcct agtgatttac ccatgcgttc tagaatttgt ctttcttttg ccaaatattc
    15301 tacaactctt gggttggttg tgatgaattc ttgttcgttg aaagcctctc caaaacggac
    15361 ttttgctttt tcgatttctt ttttggcttt taggtttaat ttttcggcat aatcctcaat
    15421 taaaacgtcg gctctatatc tttttttgga atctttgttg tcgattaatg gatcgttgaa
    15481 agcatctacg tgcccagatg ctttccaagt ggttgggtgc attaggattg ctgcgtctag
    15541 tccaacgatg ttttcgtgca tttgcaccat tgctttccac cagtattcgc ggatgttttt
    15601 ctttagttct acaccgtttt gtgcataatc atatactgca cttaaaccat cgtatatttc
    15661 gctagacgga aaaataaatc cgtattcttt tgcgtgcgaa attacgtttt taaatatatc
    15721 ttcttgtttt gccatagtgg tgcaaaaata taaaaagcaa tagtaaaaaa aggaattatt
    15781 attgtttttt gataatgatt ttataaaaat ggtttttggg tgaaattaaa ctgctaaagg
    15841 ctacaaatga gttgctaaag gttacagatt agtttctaaa ggctaaaaat gaatttctaa
    15901 aggctaaaaa tgaatttcta aaggttacaa atgacctgct aaaggctata aatgaatttc
    15961 taaaggctac aaatgagttt ctaaagggtt tttaaaaaat gtgttaaaga aaattggctg
    16021 caatttaatt tttgaattga aagattattt acttttacgt aagaaaataa cgttttttat
    16081 gtttaaacac cttatcaatt tatttttccc aaagtcttgt gcggcatgtg cttcgttttt
    16141 attggcagaa gaaattgtga tatgcacagg ttgcagacac aaacttccgt tgacgaatca
    16201 tcatttgatg gaggaaaatg aagtggtaaa aaagttttat ggtaggattc cgttggagtt
    16261 tggcggagcg ttgttttatt ttcataaaaa aggaatggtg caagaaatga ttcataaatt
    16321 gaaatacaaa ggacatgaag aaattggcga aatggctgga aactggtatt cgggagagtt
    16381 gaaaacggtt gaacaattga agtcggctga ttatattatt ccggttccgt tgcataaaaa
    16441 acggcttaga gaacgtggtt ataatcaagt ggcacaattt ggaaagactt tgagtgccaa
    16501 tttgaatatt ccttataatg actcggtttt gattcgaaaa gtgtatgcca agacgcaatc
    16561 tcaaaagaat ttattaggaa gaattgatat tgctgacact acatttgatg ttgtctttga
    16621 cgaaagtttc catggtaaac attttgttct tattgatgat gtaattacta cgggtgctac
    16681 tttagaatcg tgtagtcgag ctttgttgaa aattccgaat gtgaagatta gtattgtttg
    16741 catggcgatg acgcataatt aaggttataa attgatagat actgttttgc gaatgtgttt
    16801 tttgattaca tttgatttgt attaacaact aaatttaatt tttatgaaaa aggtttttta
    16861 cttgttttca attgtttttt taatgcaaag ttgttttagt tataaagaaa ttgagaataa
    16921 ttcgagccag tatgaaattg ggaaaaccta tagagtatat caagataaaa aaaaatcaat
    16981 aataaacatt aaaaataaaa cggatagcac tcttattgtt gtcagtaaat ttgaagagca
    17041 taaatttcca attaattcaa tttctaaagt tgataaaaga aaattttcta ttgtaaaaac
    17101 gcttcttttg ccagtatcaa ttgtttcttc aattattata ttatttgcac ttacttacta
    17161 agattttttt cgtagtggat atattcacaa atttttacaa aacatttcgt tcacactaaa
    17221 tataattgtt attttgtgac atgaaaaaac tggaaaatga atagacctta ttttaaactg
    17281 tttttattgt tagttgcgct tgctgtttcg agttgtgcca aacgaggaac gataactggc
    17341 ggagcaaaag atactttggc tccagtatta aaagcgagtt ttccaaaaaa tggttcggta
    17401 aattttcaag gaaaagaaat taaattggtt tttgatgaat atgtgaagct gaaaggcatc
    17461 agcaaacaac ttattgtttc gccgccaatg aaaacgccac cagagatttt tccacagaat
    17521 gcatcaaaaa cgattaccat aaaaattaaa gatacgctac aaccgaatac tacgtatagt
    17581 tttaattttg gtcaaagtat tgaagacaat aacgaaggaa atccgtattc gcagtttaaa
    17641 tatgtgtttt ctacgggaag ttatattgat tcgttgaaat tgaatgtgaa aatcaaagat
    17701 gctttagaga agaagtcgga taattttgta acggtaatgt tgtatgaaaa gaatgaaaag
    17761 tttaatgatt cgactattta caaggaaaca ccgcgctata ttaccaatac tttagacagt
    17821 ttgaaattgg taactataga aaatatcaaa gctggaaaat ataaattgat agctttaaaa
    17881 gataacggca ataataaata taatccaaaa tcggataaga ttggttttta ttcgggcgaa
    17941 attaccattc cgaatgatac tatatatgaa ttagaattgt ttaaagaatc cttgccgttt
    18001 aaggcaataa aaacctatca agcctcagga aataggttgg ttgtcgctac agaaggaaat
    18061 gctaaaaaca caaaagttac tataaagaac ggaaatgatg ttttgccaac agttgtaact
    18121 caatttccaa aaaaagattc gctacaagtt tggtataaac cgatgaaggt tgactcgtta
    18181 ttagtttcgg tagaaaaaaa ttcgtttaaa aaagagtttt ggactaaaat taaagagcaa
    18241 aagaaagatt cgttgagttt tacttctgat tttgcaggcg gaattccgct tagagaacga
    18301 tttggattga cgagtacaac tccacttgtg aaatttgata aaactaaaat taatattatc
    18361 aataaagatt cgttggctat tgattttact acggaatacg atgagtttaa tcaaaaattt
    18421 tatattgatt ttaaaaaaga acctttagaa aaatatacga tttcggcaat gccaaatgca
    18481 tttactgatt tttttgaaaa tgtaaatgat acgttgaact ataagctgac gactaaaaac
    18541 actactgatt atggtaattt gcggttgatt ttagagaatg caaaacgatt tccaatcatt
    18601 atagaattga cggataaaga tggaaaagta aaagctacag cttatactga aaaagaaacc
    18661 actattgagt ttatgaatat tgagccgtta ctttatacgg ttaggattat ctatgacgac
    18721 aataaaaaca agatttggga tgctggaagt tatcttgaaa atcgtcaaag tgaagaagtg
    18781 atttatttcc ctaaagaaat tgatgttcgc gccaattggg atgttgagca acctataaat
    18841 attggaatta cgaattagtt ctttgactgc gctcagcatg acaatttgaa ttcatcttta
    18901 tcgtctagaa aagctaattt gtttcgagtt tcgtctagtt ttttcttgtc caattcaaca
    18961 ataaaaacgc cttctgtttc ttgtggttct atgatataat tacccaaaaa atctatggct
    19021 tgcgaatgac caatgtattc gtggttattt ccgtcaacac caacacggtt tacgccaatg
    19081 gtataacaca tgttctctac tgcacgtgct tttagtaaaa tatcccacgc attggttcta
    19141 actttaggcc aattggcaac gtaaagcagc acatcgtagt tttctacatt tcgagcaaaa
    19201 actggaaaac gcaaatcgta acaaactagc ggacaaattt taaatccttt gtattctaca
    19261 attaattttt cttttcccgc agtataaatt ttgtcttcgc cagccaaagt aaacaaatga
    19321 cgtttgtcat aatgctgcat ttttccgtcg gggaaaacaa acaccaatcg gttgtagaaa
    19381 tttccttttt ctttgatgat caaacttcct gttatggcgc aatttttggc ttttgccaaa
    19441 tgttgtaacc acgaaatagt ttcgcctttc atggtttcgg caaccgcttt tggattcata
    19501 gtaaaacctg atgaaaacat ttccggaagc acaattaaat cgacgtcttc cataaatccc
    19561 gtgatttttt gcgataaatg actgcggttt tctagtggat tttcccagtc gattgaagtt
    19621 tgtagtaagg ctattttcat gattttatta tttaactctg tcggagttta tactattgta
    19681 aattttattt aaccgttatt tcatcaataa agataaacgc atcgccacca aatccttgat
    19741 gccattctgg taattttcca tagtttttgg caattacatt tacatagcgt gctttgatag
    19801 gtttttctaa tgtaatacta aaatcacgta tcgtgttttc ggtttctttt gcatctaaag
    19861 tgttttcaat agttccaatt tttgtaaaat taatgttatc agttgaaaca taataatcca
    19921 ctttggttgg catcaaaatc caggaacgtg aatcttgaag aaagtttgat gataaataac
    19981 ttatttcttt tatttcctgc aaatcgacta cagcttcaaa atcttgactt tgatagcctt
    20041 gccaatcacc ttttcgccag ttggtagttc caaaaatgcc atcgagcaaa ccatcatttc
    20101 ctccagcatg atattgcgga ttgtattttg atttgatgtc tatggtgtaa ttgtttggtt
    20161 ttttgaagaa ggttgcggaa atggtgttgc taggttccat aaaatcaggt tgaagataag
    20221 cttctatttt agcagattct gaaatcaaaa aaggttttga atattcgaca aaaggttctg
    20281 agttattatc ttttattatc cgataatatg cttttaagtg tttttgatcg cgtacaaaat
    20341 ctaatttatc atatgaaatt gtcacttcaa ggttattttt aaaactttta ctatttgctt
    20401 caattactgg aactgggata ataggttggt tttgctcata atgtggcttt tttaaatatt
    20461 catctaaacc aaattgactt aaataatttc cacttctttc gtcatcaatg taatcaactt
    20521 tatcatttaa atgtacttta attgatttaa aatttggttt tgttgtactc cattctaatc
    20581 ttcctggcgt tacctgataa attcccatcg aactcaacac ataccaagca ctcatttgtc
    20641 cgcaatcttc gttgccaatt aaaccatcag gagcattttt gtagaaatta tctaaaatgt
    20701 aatgtacttt ttcggtcgtt ttttcgggtt tacccacgta gttatacaaa taggccatgt
    20761 gatgacttgg ttcgtttccg tgtgcatatt gaccaattaa acccgtaaca tctacttgct
    20821 cgcgaccagt ggttttactt tcgctgttga acatttcgtc taacttggct tcaaattttt
    20881 cacttccgcc ataggcatca atcattccgg gaatatcttg tggcacaaag aaggagtatt
    20941 gccaactgtt gccttcggta taattgttgt ttatttctct tggatcaaac ggtttgtccc
    21001 aaccgccatt tttctttggt cgcataaaac ctatattcca gtcgaaaaca ttcttccatg
    21061 attgtgaacg tttcatgaag tattcgtagt cttctttttt gtttaaaatt tgcgccattt
    21121 gagcaataca ccaatcgtca taagcatatt ctaatgtttt agaaacgctt tcgtgttcgt
    21181 cgtctatgga aataaagcct tgctttttgt aagcatccaa tcctaaatga tctaacatcg
    21241 ccgaatgctt tgcagcttca aacgcttttt cataatcaaa ccctttaatg ccttttgcca
    21301 ttgcatctgc cataaccgaa acggaatgat aacctatcat acaatcggtt tcgttagacg
    21361 ccaattccca aacgggtaat cttccgcctt gttcgtattg ttttagaaag gtattgataa
    21421 aatcggctgt tcgtttttta tcaatcaatg tatataatgg atgtgcagca cgaaatgtat
    21481 cccaaagcga gaacaccgtg taataatcaa atccttcgcc ttcgtgaact ttgttgtcgc
    21541 gtccgcggta ttttccgtcg atatcttgtg cgatattggg ttgcaccatc gtatgatata
    21601 aagccgtgta gaaaatggtt agtttatcat tattgctttc cgagatttca atttttgata
    21661 gctctttata ccaaagttgt tcggctgctt ttttaacttt attaaagtcc caatctttaa
    21721 tttctgaagc atttaattcg gcaccttcat aacctgttgg tgataacgtt actttaactt
    21781 ttaatttctc tcctttttct acttttcttg aaaaacttaa agccaactca gttccattaa
    21841 atcgccttcc tttttcccca ttgcatttaa ctttgtttat tccaaatggt ttttcaaatt
    21901 caattaaagc aaaaacatat tgatctcttg cccaagcttc gcttctacgt ttcacccaaa
    21961 ttcttttttc atcaattatt ctaacttcac cttccaatag tttatctcta tgatttaaat
    22021 ctaaaatgat gttggcttgt ccagctttat taaaagtata ttcgtgaaaa cctactcgtg
    22081 ttgaggttgt aagtcggaca tcaatattgt gtttgtctaa tttgactgaa tagaaacctg
    22141 ctgatgcttt ttcgttagca tgagaaaata tcgaactgta ctcttttggg tcaaaacttg
    22201 gttctcccat ggttggcata agcatgatat cgccaaaatc agaacatcca gttccgttca
    22261 agtgcgtatg tgaaaatcca taaataacat tgtctgtata gtgataacca gaacaaccat
    22321 cccaagaacc atcaattctg gtatcgggcg aaagctggac cattccaaac ggaactgttg
    22381 ctcctggata ggtatgacca tgtccgccag ttccgataaa tgggttgacg tattgttggt
    22441 aattttgact nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaccttctt
    22561 cgtccatttg acgcaccatg tttaaaacac gctgtgttgc ttcaactttt ccttgcggca
    22621 acaaagcata ttgtgacact tttgcaccaa caaatagcat tgctgaaccg tttttacaac
    22681 ttgccacaca agcaccacaa ccaatacaag ctgctgcttc aaaagctttg tctgcatcgt
    22741 ctttaggaat tggtgttgca ttagcatcaa tggtgtttcc agaagtattt accgaaacga
    22801 aaccacccgc ttgttgaatt ctgtcaaaag aacttctatc aactactaaa tctttgacca
    22861 ccggaaatgc tttacttctc catggttcga tataaatcgt ttcgccatct ttgaacttac
    22921 gcatgtgcaa ctgacaagta gttgttctgc tatctgggcc atgtgcttga ccattgatgt
    22981 acaacgagca cattccgcaa attccttcgc gacaatcgtg gtcaaaagca acaggttctt
    23041 gtctttcgtt aactaattgt tcgttcaatt ggtctagcat ttctagaaac gaactatcag
    23101 tcgatacatt gtctaattta taatctacaa ttttaccttt ttctttagcg tttttttgac
    23161 gccaaatttt tagttttata ttgatgtttt tctttgtagc catgatttct tatttgtagt
    23221 ttctagcagc aattttaata aactcgtatt ttaattcttc tttgtgaaga ttttctttgg
    23281 tgatgtcaaa tcccatgtat tcccatgcac caacaaaagc catattcaca tcatcacgaa
    23341 gtgtttctcc ttcgctgtct tgatattctt cacggaaatg tccaccacat gattctttac
    23401 gttgcaaagc atccattgcc attaattgac ctaaatcaat aaaatcggca acacgaagtg
    23461 ctttttctaa ttcaggattt aattcatctg cacttccagg aacgtaaaca tctttataga
    23521 attcttcttt taaagcagca atttcttcga ttgcttcttt caatcctttt tcgttacgag
    23581 ccatacccac tttgttccac atgattaatc ctaaacgttt gtggaaatgg tcaactgatt
    23641 ttgaaccgtt attgtttaag aatttatcga ttgaatcttt aactctgctt tcagcttcat
    23701 caaattcagc tgattgagta gaaattttac ccgttctgat ttcgtcagct aaatagttag
    23761 aaacagtata tggtaaaacg aaataaccgt ctgctaaacc ttgcattaat gccgaagcac
    23821 caagtctgtt tgctccgtga tcagagaagt ttgcttctcc agctacgaaa caacctggaa
    23881 tagtagattg taaattataa tcaacccaaa ctccacccat tgtatagtga actgctggat
    23941 aaattttcat tggagtttca tatggatttt cgtctgtaat cttttcatac atttcgaata
    24001 agtttccata tttttcttca atccattgtt ttcctagctt gataatttct tcttcagaag
    24061 gattgtgatt tccttttgca taagctactt ctttaccttt atttctgatt tctgttgaga
    24121 agtcaaggta aacaccttcg ttggtgtcat ttgcttcgat tccataacct tcatcacatc
    24181 tttcttttgc agctcttgaa gctacatcac gtggaactaa attaccaaat gctggatatc
    24241 ttctttctaa gtagtaatct ctatcttctt ctgcaatttg cgttggtttt aattttcctg
    24301 cacgaatagc ttctgcatct tcttttttct ttggaaccca aattcgtcct gaattacgca
    24361 atgattcaga catcaaagtt aattttgatt gattggttcc gtgaacagga atacatgttg
    24421 ggtgaatttg cacataacat ggatttgcaa ataatgcgcc ttgtttatga attttccaac
    24481 tagccgttac attacttccc attgcgtttg ttgaaagaaa ataaacattt ccataaccac
    24541 cagaagcaat aattacagca tgagccgaat gtctttctaa ttctccagta acaaggtttc
    24601 ttgcgataat tccacgagct ttaccgtcaa cttttactaa atctagcatt tcgtgacggt
    24661 taaacatttt tacacgtcct aaacctattt gtcttgataa agcagaatat gcaccaagca
    24721 ataactgttg tcctgtttga cctgccgcat aaaatgtacg ttgtacttga acaccaccaa
    24781 aagaacggtt gtctaaatta ccaccataat cacgagcaaa aggaacacct tgtgccacac
    24841 attggtcaat aatatttgca gaaacttctg ctaaacggtg aacatttgct tctcttgctc
    24901 tataatcacc accttttatg gtatcgtaaa ataatctgta tgttgaatca ccatcatttt
    24961 ggtaattttt tgcagcgtta attccacctt gtgccgcaat tgaatgtgca cgtcttggag
    25021 aatcttgaaa acaaaatgct tttacattat aacccatttc accaagtgaa gcggcagccg
    25081 aagcaccagc taaaccagtt ccaataacaa taacatctaa ttttggacgg ttgtttggag
    25141 caactaattt taaatggtct ttataatcgg tccatttttt atctaatggg ccttgaggta
    25201 ttttagaatc taacttcata gtatatgttg ttgatttatt ttaagaaaaa atgaatgtaa
    25261 actggaataa ctgcaaaaag caccggaaca acgattgcga aaaatgtccc aaatcctttt
    25321 attgcggaat tatatttagg gttatttatt cctaaagatt gaaatgcgct tttaaaccca
    25381 tgtaataagt ggaatgccaa tgtagccatt gctaaaacat aaagaatcgt gaaaaccaat
    25441 ccttcttttg gaatgacacc catgaatttt gcttcacttt taaagaaagc aatcgtgatt
    25501 ttatacaaat ctttatagcc ttcaaataca atttcaccat caactttatt tacaagagtt
    25561 gtgttattta ccaattgtaa atcgtatttt ccttgagttt gttcttttac agtttttata
    25621 aaattttctg aaattacata ttcaccgtta gtatttaaaa atggaatagg agaatctttt
    25681 gttccaacta gttgaatttg tttttgcccc atgccattat cggtagtttt tgcaaccaaa
    25741 ggcatgttac gatcaaaatg catttttgcc caaaaattta ccatgtgtgt aataataaac
    25801 actaaaataa tagttcctaa aactgccatg tttctagagg aaaaactact gttttttgat
    25861 ggagtgtttt tggcataacc aattggtctt gcttttacat tttgatacgt taaaataaaa
    25921 ccatcaattg cgtggaaaag aatggaaata taggttaagt acgagagaag ttttaccgct
    25981 ggattagtcg tcataaataa tgcatattta ttaaaattca atgcattgtt cggtactaat
    26041 aattgtaaat ttccagctaa atgacctgtt aaaaacaagc acaaaaataa acccgtaaga
    26101 gccatccagt atttttttgc taaggatgac tttaaaagtg cagattttgc cataagattt
    26161 ttttaattta ttttaaaaag acagacaaaa ataatgttaa ttgactacaa ctacaacctt
    26221 ttcgaggtaa tttataatga atttaaagtg tgtttaacgt ttgttgattt tcaagttgtt
    26281 aaagtttcta tttttctagt tccgtataat aaattatttc acataagttg gaattaaacc
    26341 tcgaattccc atatcaacca cattttctcc aattgaaggt tcaaatttac cgtctgaagt
    26401 tacttcttgc cattcaaaac tattgttagt tgaaagattt aaagtaacca caacatcatt
    26461 ggtttcattc cctgtaatga ccaagttggt tggaaatttt ccagtaacta cgcacgaacc
    26521 tgctggaatt ggtgatgtgc tagctaatgg atttggaact gtagttgcat ttgctggtgc
    26581 ttgtccagag gaagaataag cttgattatt taacgcaaat gcccaaaatc cttgagctcg
    26641 attaccgtta acatcaaaaa aattattacc aattgaaaac gaaccaatat aagtgttaaa
    26701 tccaataaaa cttgccagag ttccttgata atcgacattc tgatgacgaa tgttgatttg
    26761 ataattttga taggataatg aaactctcaa ccattcataa cttcccgaat tgagttggct
    26821 taatggtatt tttaagaaag tttcaccttc tgccactatt tttgattgat caaaatccaa
    26881 tgcattactg ccaccaataa tcgtttcggg agcatgataa acaatacttc ctgaaccaag
    26941 ctgggtagtt gtgtttggag caaattcaac ataatgagct gaaatagtgt tgaattgagg
    27001 tgattgtgcg gcatgtcctg cggctaaaac agagggttgt cctaaattat tcaatcgaat
    27061 ttgtgttggg tcaaatttaa atttgataat caacatcggt tctgagtttg aatcgtcttt
    27121 actacacgaa aataaaagca aactcaaact tgaaagtgtg ataaaaagca gctttttcat
    27181 aaatttattt taataggttt tggttgcatc tatatctata acgaaaatat agtctgcttt
    27241 agtataattt tctttgtcta atttcgaaat atcatcttgt tcaacagttg aaatggtttt
    27301 tatggttaaa ttttcttgca attttttcaa gtaccaataa atcccttcaa aattgtcgct
    27361 gtgataggtt ccgttaaagt gaatgaacaa agcatttttt gctaagtttt tattaataaa
    27421 atgtgccata gttgcatctt ttatcgcttg tgctttgggc attttttcgc cagcatgttc
    27481 cgtcatttcc atcatcgatt gataattagg aagagtaata tcaaaaggaa ttggtaatgg
    27541 agcaatccac aatttttctt catcagtcaa attatctaat gcttgaaaat catttttata
    27601 aaccatcgat gcgtatcttc taggaatatt ggtagcaatg aattttagct ttcttttttt
    27661 agcatagtca accaaaggtt tgtaatctgt tttataatta ttccaaagcc tagcggtgct
    27721 atccaattgt ttttgattaa tctcatcatt caaatattga ttaacctgct tttgattatc
    27781 cgcttctaac atttctgcac caagaatcac ttgctttttt tgagctaaat ctttcgtgag
    27841 ttctaattgc aaccaatgca caatcgaatt attatgatat tctccaaaca aaagaacatc
    27901 tgcattttca atgtttttaa gtagtttttt ataacttact tttttaccat ttttatcaaa
    27961 caattgatac ggacttttgt tttgagcaaa atttaaaaaa gaaacaagaa ataagataga
    28021 aataagtgat ttcatgaaat attttttcta aaattattca tttattatta aattcaaagc
    28081 gactttttta aaaaagaata cttttgtagt tatagattat aaaatggcaa ctacaaaacc
    28141 taaagaagca tttttattgt tcgtcaatca atttattgat aaaacagatg ttgcaatgca
    28201 gttacaaata aaagctgctt tgagttcatt ttcattctca tttttaaaga aaaatgattt
    28261 tttagtccaa gaaaatgaaa tatgtaatca tttttgttac atcgaaagcg gtattttgca
    28321 acacgccatt gaagtggatg gcgaagaaaa aactacttat cttgggttgc gaaattcggt
    28381 tacttcttcc ttaaaaagtt ttttaaatca aacggcttct caaaaaagca ttaaagcatt
    28441 ggttgataca gaactttgga taattgattt aaaaaacttt caagagttat tacaaaataa
    28501 tgctgctttt catcattttt atttcaaatt gatagaaaaa caaatcatgc taattgacga
    28561 ttatcgcata gatttattga cattatctcc cgaagaacgt tataaaaaac tactcgtcac
    28621 tgagccaaaa cttctccaag aagttccttt gcattatttg gcatcctttt taggaatttc
    28681 caatcgacac atgagcagaa ttcgcaaaaa tataaaataa cgccatttgg ctactctata
    28741 gtttttttct ttatgtaaat ttggggtatt aatttattta aaaatttaaa aaatgaaata
    28801 ccatccaatt gaccgcaatt tatttataaa aaatagagca aaatttaccg ctggaatgaa
    28861 accaaatagc gttgccgtgt ttaacagtaa tgatatttac cctgtttcgg ctgatagcac
    28921 tttacctttt gcacaacatc gtgacatctt ttatttgtct ggtgtagatc aagaagaaag
    28981 cattttgttg ctttttcctg atgctccata cgcccatttg aaagaaattc tttttctaaa
    29041 agaaaccaac gaacacattg ccatttggga aggtgagaaa ttaaccaagg aacgtgctta
    29101 tgaagtttca ggaattaaat cagtgatgtg gttacaagat tttcacaaaa ccttaaatga
    29161 agtaatgacc tatgcagaaa ccatgtatat taacactaac gaacattata gagcatcggt
    29221 agaaaccgaa acccgtgaag ctcgttttgt aaaatggtgg aaagaaaatt atccggcaca
    29281 caaagtagaa aaatcaaatc ccattttgca acgcattcgt tctattaaag aaagcgaaga
    29341 actagattta attcagaaag cgtgtgacat tactgaaaaa ggattccgta gagtattaaa
    29401 ttttgtgaaa cctaacgtaa tggaattcga aattgaagcc gaatttatgc acgagttttt
    29461 acgcaatcgt tccaaaggat tcgcctatac gccaattatt gcatctggta ataatgctaa
    29521 cgtgttgcac tatattgaaa acaatcaaca gtgtaattcg ggtgacttaa ttttattgga
    29581 tgttggtgcc gaatacgcta attattctag cgatatgacg agaatgatac ccgtttcagg
    29641 aaagtttacc aacagacaaa aagaagtgta caatgccgtt ttacgcgtaa aaaatgaagc
    29701 tactaaaatg cttgttcctg gtaatttttg gaaacaatac cacgttgaag ttggtaaaat
    29761 catgacttca gaattattag gtttaggatt attagacaaa gccgatgtac aaaacgaaaa
    29821 cccagattgg ccagcctata aaaaatactt catgcacgga acatcgcacc atatgggatt
    29881 agacacacac gattatggat tattatacga gccaatgcaa gccaatatgg tgtttactgt
    29941 tgagccaggg atttatattc ccgctgaagg tttgggcatt cgtttagaag atgatgtggt
    30001 tattcaaaaa acgggcgaac cattcaattt aatgcgcaat attccaattg aagcggaaga
    30061 gattgaatcg ataatgaatt cttagatttt agttaaggcg tttaaaggat tataagttta
    30121 aaaggcggtt tacgaaagtg agccgctttt ttttccctat aagtcatcta agaagatttg
    30181 agttttcttt tataaaactt aaatgattta tagggttaaa taaattattt ttgcatcaat
    30241 gaaaaccacc atctacaaaa acaccaaaat ctctttcact gatcaaggca aaggcactgc
    30301 agttgttttt cttcatggtt ttttagaaaa taaaaccatg tgggatgctt ttatccccga
    30361 tttggtcaaa aaaaaccgca tcatcaccat tgatttatta ggtcatggtg atagcgaatg
    30421 tttgggatat gtacattcta tggaagatca agccgatatg gtacaagcta ttcttcatga
    30481 attaaaaatt agaaaggcag tttttattgg gcattctatg ggtggttata ttgctttggc
    30541 ttttgccgaa ttgtatcccg aaaacataaa aggattagtg cttttaaact ctacttctcg
    30601 cgccgatagt aatgaaagaa aggtaaatcg tgatcgagcc attgtagctg ttaaacaaaa
    30661 ttataccaat ttcgtccgaa tgagtatcgc caatcttttc agcgaagaca accgcaaaaa
    30721 aatgatagat gacattgaaa aagtaaaact cgaagctctt aaaacaccgc ttcaaggtat
    30781 cgtagcggca cttgaaggca tgaaaatcag aatagataga gaagttattt tgcattttgc
    30841 tccgtttcca atccaattaa ttttagggaa aaaagatggt gttttaattt acgaagagaa
    30901 tttagaacaa atagaaggaa ccaaagtaaa actcaccact tttcccgatg gtcacatgag
    30961 tcatattgaa aatcaatttg aattaaagaa agttttattg agctttttaa aagggatata
    31021 aaaaaaacaa ctcactttcg tgaattgttt tctaatgatt cttctaaatt agagtttcgc
    31081 tcttgaaatc tcttgacccg ttggatactt aatagcagca tcatcttctg cggtaataaa
    31141 aatttcatcg ggttcataag gcgtaatcgt ttcaaatgat gcttttgatg aagcatcggt
    31201 ctcaaatttt cctaaatttt gcaaaccgtt agatttcgtt tggagccaca ccaaataaaa
    31261 ctttttggaa ggcgataacc tttcgggtga agttaaaaac tttgccttca gctttagttt
    31321 actgtttcca ttatcatctt tggaggtttt gaccgtaatt tcggctcctg gtgtaacatt
    31381 agaaactgga aatttgactt ttgatgcaca agaggctaaa atcaagaaag ttaaaataat
    31441 tatttttttc atatattttg aattttaagt tcattaataa tatacgaaaa aaaaacgaca
    31501 attcctaatt atagtagttc ttcaaagggt ttgtctctat caaaaatggt taaaagcaag
    31561 tttacaattt catttaaata attttccatg atttcgggag ttattacttc ggttgtaacc
    31621 ttatctactt taaaattgaa gggaagaaac ccagctttca tgtttttaaa cgatatgatt
    31681 cctgcttcta tctgacggtt tttagcttcg ggttcataca taaaagcata tgccaaaacc
    31741 tgaatgattt tatcgttttt aatgtcagca gttaaaccat tccaatcata aagcgtcata
    31801 tttctacctt caaccttacc tgttttataa tctataatcc ggatttttcc gttatgaagt
    31861 tcaactctat caatatttcc tttaattaag actggaaaag gcaaacgttt gtcttcaatg
    31921 gttcgttcgt aagaagtttc tagcgacaaa acttgaattt catcaccatt ttctaaagat
    31981 tttagttcgc tattgaggaa attttccaca ttgcgtttgg cgacttcaaa agcaagaagg
    32041 tttcttcccg tttttatttc gccttctttg tattcttttt taaattgaag ggttacttcg
    32101 tttgcgattt taggaaatga atttttaatg tcatcaatcg ttaaaattct atttaaaaaa
    32161 ggtttgtaca actcttctaa agtagcatga ataatggttc ccaaagtatt tacagcaata
    32221 ttttcttcta cttcatcggt ttctttgata cgaagaatac gttgaaaata aaattgtaat
    32281 gggtttcgaa tataacttgt taacgaagaa ggagaaaacc cattatgtgc aatttctttc
    32341 aagcgttgct gtaccaattc tgatttctca attatgattg gccgattcgc gatttcgggt
    32401 acagctggat tataaaatga atgtttcagt gtatgattgg gttgtttttc aatctctaat
    32461 tgcgtgatga acctactttt ttcaccagca tcaaagccat cgctgtcgga attgtataat
    32521 aagtaaatat ttttggcacg ttgcaataaa tgatagaagt gatacgtgta aatggcgtct
    32581 ttttctttga aagttggcaa accatattca cgttttacat catagggaat aaaagaattg
    32641 attgattttc ctgctggaaa tttgccttca ttaaccgagg tgataattac agtatcaaaa
    32701 tccaacactc tgctttctaa aacacccata atctgtaatc cttgcaaagg ttcgccttca
    32761 aaagaaactt cggctacatc aatcacttgt ttgtaaatag cgaatactgt ttttaaatcg
    32821 gtaaccgaat tttgatggtc gaaataagaa atcaatttat tgatgacttt gtatattgaa
    32881 tagacaaaag cagttgtgat tttttcttcc tcattttcac gatttaattt gttcttaata
    32941 tgtactaata tttcgcctat gctttctaaa aacttaactg gattggcatc ccatttctga
    33001 aacaataatt tataaagtgc attttctttt ccatataatt cgtgcaattt attccaagag
    33061 ataaaagaaa aattattttt attgattttg tttaccaagt cataagcatc aacatgtggc
    33121 tctattaaag gattggttag cacatcgagc acatctttaa aatacatcgt ataactcgag
    33181 ctgtttcgat ttgtggtatt gacatgaagt ttaaacagct tcgcaatcaa taattgcgca
    33241 gggttgtttt tacttgaaaa gcccatagta acattgagtg catctacagt agatggtaaa
    33301 gaatagagta tcggtaagag taaattttct tcgccaagaa cgatagcaac attctgaaga
    33361 ttttcttttt cggcatattg atggattagg tttccaacaa ttttggcttg accaattgct
    33421 tttggggtgc tgatgatttc aatctttttt tcagttgcaa aatctttagc aatccattga
    33481 tagggattgg ttttatagta gttccactct tttttaaacc tacgttgaaa caaacctgca
    33541 tcatgaaatt catctcttaa gaatacctca tccaaatccc aaaaaacttc cgctctatca
    33601 atggctaaaa aatgttgaac aatcaattct tctgcttgat ttaaagcatt aaaaccagca
    33661 aaaataaaaa ttttattttt taaattatcg gtataatttt ttagattttc tttggcctca
    33721 cgataaatta acccttgata acctatacct ttgttgaata aatgtttgta aaaagaatga
    33781 taatagagcg gcaatttttt ccaaaacaaa aggtgttttt caattaagtt ggttttcttg
    33841 ttactatcaa ctgaccagtg ttcgatttct tttatgtttt caagatattt tagaatttta
    33901 ttgggatcta aaagataacg gtcaatttca ttaaaatctt gtaataaggt ttttgcccaa
    33961 tttgcgaagt tttcaaattc gtctagtttg tcttgttcct caactttgca atagacttca
    34021 tagaactcaa ataataactc taccgaatca atgcttctta ttccagaaat agtttgaata
    34081 aagtcttcaa tggaagtgat ttctggagca aaaatcgttt tatctgtagc aagttgtaat
    34141 tcatttatca aaaaaacttt tgctctccta tttggtaaaa taatcacaac atccgaaagg
    34201 ttttcagaat gtttcttaat tatagtttgc gctagttgtt tgagaaaagt tgtttttgac
    34261 attgtataaa aataaaaaaa cgtcccgaaa ttcgggacgt ttttatgaaa atattttaaa
    34321 aataaattat tttactaatt tcacttcaac tcttctgttg ttagcttttc cagctttcgt
    34381 tttgttagtg tcaattggca ttgactcacc aaatccttta gaagataatc ttgaggtatc
    34441 tataccgttt tctactaaat agtttttaac agcagctgct ctatcttcag atagtttttg
    34501 gttagcagcg tctttaccat cgctatcagt gtgtccttct aaagagaatt tagaatttgg
    34561 atactctttt aagattcctg taatagattg tagtactggg tatgtttgtt gttggaaaga
    34621 agctttacca ctatcaaata agatagtttt agcataatcg ttcaatcttt ttacaacttc
    34681 gtcagatact tctgggcaac cattgttagc aacagttcct ttaacgtctg gacatttatc
    34741 atctttgtct aatacgctat caccatctct atctggccaa ggacatccac cattttcttt
    34801 aggaccttta acgtctggac atttgtctga tttatcagca attccatctc catcagtatc
    34861 aggacaacca ttcaatgatt ttaaaccagc tacatctgga caagcatcat ctttatcagc
    34921 gattccatca ccgtctgtgt caggacaacc gttgaattca actggaccag caacatctgg
    34981 acaagcatca tctttatcgg cgattccatc agcatcagta tcaggacaac cattaaattg
    35041 ttttaaacct gcttggtctg gacaagcatc atctttgtca taaattccgt caccatctgt
    35101 atctttccct ccaaatttga aagtaagacc agcaaagtgc tgcatgtgag ttggcatgtc
    35161 tgctctacta tcttcaaatg agtacttgta agtagcacga tacgataaac ctacgttttc
    35221 agaaaaccag taagccaaac ctaaaccacc atttacagtt cctgcgctag aaacgtcacc
    35281 taaccaagta taaccacctc ctacatgaat agaaggatca atagttttag atttgattac
    35341 gttcattaag ctgtaattga ttacaccatc aactgcgtaa taattaacat ctccaggatt
    35401 ggttactgta taatcgtttg ttccaactct tggattaaca aatttagtta atttgttaat
    35461 agatcctgta actccaaaag agaagttact accaacgtat ttagatacgt ttacgaaaga
    35521 tacagaaggc aaaatgttcc aattgtcttt tgcattgaaa aattctgaaa actgatcttc
    35581 taatttagaa gctgcactaa ctctagtgtc aactgcattt accccaaaag atatagccca
    35641 tgggttgttg ctgtcttgag cttgagaact gattccagct aacattacca aagcaacgaa
    35701 taatttgtta aggtgtttca tacttaattt gattagatta taataattat aatagagcaa
    35761 aagtaatacg tattccaata atatcaaaat ctaattgtta aaatgtgtga attatataac
    35821 tattagagta aaattttgaa ataaaaatgc cgctatttta taacgtttag cttcttgcca
    35881 acctccgtaa aggctaaaat tgctctgtct aaatgctctt tagtgtgtgc tgcagatagt
    35941 tgcactctga ttctagcttt tcctttggga actactggaa aaaagaaacc aattacataa
    36001 atcccttttt ttaataattc atctgccata acttgtgata atttagcatc atatagcatt
    36061 acaggaacaa tggcagaatc accatcgata atatcaaaac ctgcattttt cataccttct
    36121 ttgaaataat tggtgtttga ttctaattta tctcttaaag aagtatcttt tttcaacaat
    36181 tcaaatacct taatagaagc accaactatt gctggtgcta atgaatttga aaacaaataa
    36241 ggtcttgaac gttgtcgtaa aatttcaatg acttcttttt ttgctgtagt atagccgccc
    36301 attgcaccac caagtgcttt tcctaaagtt ccagtgataa tgtcaactct tcccattaca
    36361 ccttttgctt ccaaagttcc ttttccagta gcaccaataa atccagctgc atgacactca
    36421 tcaaccatta ccattgcatc atatttatcg gctaaatcac aaattttatc caaaggtgcc
    36481 actaagccat ccattgaaaa aacgccatcg gtaactatta atttaaaacg atgttctgct
    36541 tcaacggctt tttttaattg catttctaaa tcttccatat tggagttttc ataacgatag
    36601 cgcgctgctt tacataaacg aactccgtca ataatggaag catgatttaa actgtccgaa
    36661 ataatgcaat cttcttctcc taataatggt tcgaaaacgc caccatttgc atcaaaagct
    36721 gctgcataaa gaatagtgtc ttcggttcca taaaaatcag caattttttg ttccaaggtt
    36781 ttgtgaatat cttgtgttcc gcaaataaat cgcaccgatg acattccgaa accatgagaa
    36841 tccaaagcat cttttgccgc ttgaattact tcgggatgcg atgacaaacc taaataatta
    36901 ttggcacaaa aatttagaac cgtctcgcca tttaccataa tctccgctcc ttgtggcgaa
    36961 gtgatgatac gttctttttt atacaaacca ttttcttcga tggcttttaa ttcgttttgt
    37021 aagtgttgtt gtattttccc gtacatactt ttaattttgg tttgtagttt attgtttgtg
    37081 gttacaaatt tacaatttca attttgtctc caatgtaaac caacgttttt ttacagactt
    37141 tatagcccat attttcaatc gcaatttgat aatggtctaa ttgagctttg tgtttaggca
    37201 aatgttgtcc tgttttataa tctaatagaa aaacttcatt ttctttagaa accgccattc
    37261 ggtcgggttt tatgatgcca ccattttttt gaataattgt tttttcgttc aaaattttat
    37321 gctctgttgc aaaaaaagat cgcaattcgt catgaccaac tatttgttct aaggtttttt
    37381 ggacttctcc tttttgagca ggaacaatca atccgctttc aatagcttta gttaatgcca
    37441 aatcaatatc ggctttggtt tcaataaacg ccaatatttc gtgcaaaaca tttccaaatt
    37501 cgattgcttt ttgttgaatg gtattccaca ttaaactttc tctttgcgca attttaatat
    37561 ttttgggatc aagtgtttcc gccatttgag gaatggtttg cgttttatct tctattgtgt
    37621 ttggtgccga aagtttattg ttttctcccg aaagatattc cattttccct tcttcaaatt
    37681 cgcctttttg aattagatag ttaataaaaa atgacgccat attctttgga tattcacctg
    37741 tcgaatttga gcgagtttgc atttcagaaa taatatataa ttgttcttcg gcacgtgtta
    37801 aagcaacata taacacattg atgtcatcca ataattcttc ttgttttttg tgcaaataca
    37861 cttcggaagc ttgttcgcca taattttcaa cacccgaatt tttatcgacc aatacttttg
    37921 gcaaacccat ttcttcatca gcaaatagcc aaattttttc ttttggacct ttactgtaat
    37981 cttcatccgc aaacggaaaa atcactactg gaaattccaa ccctttcgat ttatggatgg
    38041 tcataatttt tacggcatca tttccttcgg gtgacggaat gctcatttta tgtgaattcg
    38101 catcccaata cttcaaaaaa tcacctattc cagcttgttt ttttaagtct tgttccaaaa
    38161 ccaaatcttg aaaaaattgc acataagccg ttcttttttc aatagcaatt attctagaaa
    38221 tgatgatttc ggtggcttca tacaatgatt ttttgcgtat atttttaaag gaaaaagtaa
    38281 tcccaaattc tgttaaccaa ttttcaaaat cattttcggc tgaaaactcc attccttttg
    38341 ccataaaatc gtgaatgggc aatttatttt cttttacaac tgtcgcattt cttcctaaat
    38401 aatacaagaa attggctttg gcttccaagt cggaataatt gttcaaataa cgcaacaaat
    38461 ccacaatcgc tttgacttcg cttgatgaac cgagcaacaa actttcggat gataaaatag
    38521 gaattccttt ttcggttaaa taattggcaa gtatcgttcc ttgcactttt tttcgagtta
    38581 gaatggtaat ttctttattt ttaaatcctt tttcttttgc gtttaaaata ctttcaactg
    38641 ttttttctaa ataaagctcg tcttttgatg ggttttcttc ttcatcaaaa ttgtttttgt
    38701 ctaattttgg tataaacgaa atggaaacaa aaccaccttt tttatcatta cttttttgat
    38761 gcgaatgatt ggcatacaag tccttatagt cttcattttc aaattgattg gacagaaaac
    38821 caaagaatgc attgttgaat tcgattactt ttgcataact tctataattg gtttttagcg
    38881 attcggtgtt tttttcaacg ttcggaaaag gattttcatc tttactcaat gcaataaatt
    38941 gttctgcttt tccgccacgc catcggtaaa ttgattgttt tgggtcgcca acaattaaca
    39001 atgttccttg tgttccttct tcatcttgac ccgaaagcga gttgccaatt aatggaatta
    39061 aattttgcca ttgcatctcg gacgtatctt gaaactcatc aataaaaaaa tgacggtatt
    39121 tttcgcccaa acgttcgtat ataaaaggtg ccggttggtt ttgaatttgc tcataaatca
    39181 attgattgaa ttcggcaatg gataaaatat tttgttcttc ttgaatggat ttcagtttgc
    39241 tattcaacgt gtttaaaagt gaaagcggcg tgatgttttt taaaaacgct tcatagaaaa
    39301 ataacttttc atattttttg tagattttat ctaaattctc aattaaatct ggaataatat
    39361 tttcgataat ttccttgtct tttgcagttt tgttgatttt gatgtcttca aattcgcgat
    39421 aagttttatt ttttggattg aattcttttt cactcaattt ttttaaatga ttgggaaaat
    39481 gtccggctga aaacgatttt atatcaatgc ctttgctgtc gattaattcc aatgcctttg
    39541 cagcaagatt tgctgtttct tctaagattt cttcacacaa atcgacgagt ttctttttaa
    39601 tttctacaaa atcttcgatt gatttattgt gaaaatgcgt gatttcttcg cgattgtttt
    39661 cattcaatat caattttccc gtttccatga tttctctaga aatatcccaa gatttgtcgt
    39721 cgttggtttt ttctaaagta taatttacca ataattctgt gagttgtttg tcgtttccag
    39781 cttcagcaat aatggcatca acagcttcag tcaacagatt ttcagtatca agcgaaacct
    39841 caaaagtgat tggtagattc aaatcgtgtg caaaacttcg aatgactttg tgtgtaaatt
    39901 tatcaatcgt agaaatgtca aaagcggcat agttgtgaat taaatgcttg atgatgcttt
    39961 tggctttcaa atgaattgtt tgcaaactca aaccggtttc ggagtgaata acttgcatca
    40021 gttgatgtgc tttatcggaa gggttttcct tggcaaactc tgaaaggttt tgtaccacac
    40081 gacttttcat ttcgtgaacc gctttattag taaacgtgat ggcaagaata tttcggtagg
    40141 catcaggttt tttagcaaga agaatgattt ttaggtattc ttttaccaag gtataggttt
    40201 tacccgaacc tgctgatgcg tcgtagattg agaatggttt tttagtcatt atttcaaaat
    40261 ttcataaata aaattcaatt tagaatcttc gccatgataa gcaacttcta attcagcaat
    40321 ttttttggca ccaatttttt caatggcttt ttgtgaacgg aaattggttg cgccaatatg
    40381 aaaatgaatt ttctctacaa attgaaaagc ataatcaatc attattttct ttaaagccaa
    40441 gttgtatcct tttccccaaa actttcgtcc gataaaagta taaccaatta aaacagtttt
    40501 gtttatttcg tcataatcat aaaaacgact gctaccaacg acttcgtttg ttttggtatc
    40561 taaaatgaga aatgcgcctt tagaaaccat ggcgccatca aaaaaatttt gaaaaacatt
    40621 tcgttgataa cgtaatttat taggatgttg ttcccaaagt aattcatcat ttgcaacttc
    40681 atataattct tcaaaatgat tttctttcaa aggaatgagc tgaataagtt cgttttgaag
    40741 gttttttggt tgccaatcaa gctgcatata agttttaatt attcgttaat tgataaatag
    40801 aattttcttt tacgaagtta aatgtttaaa tttgaattca attaattact aattaaaaaa
    40861 ataaatatta tgtcatttga attaccacaa ttaccttatg catacgatgc attagaacca
    40921 catattgatg ccagaacaat ggaaattcac cattcaaaac atcataatgg ttatacaacc
    40981 aatctaaatg ctgcagttga aggaactgat ttagaaggaa aaactattga aaacatttta
    41041 attaatcttg atatgaaaaa tgcagccgtt agaaataacg gtggcggatt ttataatcac
    41101 aatttatttt ggagagttat gtcgccagac ggtggcggaa aaccaacagg agaattagcc
    41161 gatgctattg atgctgcttt tggaagtttc gatgctttta aagtacagtt tgcaaaagct
    41221 ggtgctacac gttttggttc gggttgggct tggctttgcg ttcacaaagg tggaaaggtt
    41281 gaagtttgtt ctactccaaa tcaagataat cctttaatgc ccgatgtcgg ttgtggagga
    41341 tttccaatat taggcatgga tgtttgggaa catgcttatt atttacatta tcaaaatcgt
    41401 cgtccagatt atattgaagc attctttaat gtaattaatt ggactgaagt ggctcgatta
    41461 tatgctacag aaaaataaga aataaaatca tttgataaac aaaccgattg tgaaagcagt
    41521 cggttttttt tgtggttggt ataaaatgaa acaggtagaa ttgcttctac ctgtttgccc
    41581 caaatctacc ataaacttaa cctacactaa atactatggt accgcaaatc taataacaag
    41641 ttattgaata ttagtatatt ttcttaaaaa attattaaaa aaagatgaaa tttatggtaa
    41701 atagtaggat tttggaggtg gacgacacga tttggtactt atataaaata aaaaaggtga
    41761 aaatttcttt tcaccctttt tgccccaaat ctaccataaa cttaacctac taatgctatg
    41821 gttccacaaa tatatagtga gtgttatttt tgaaaaaata tttctgatga actgctttaa
    41881 aagtcgttaa agttcaaaaa atgttaaaat ttaatacgct ctagcattat ttccttcata
    41941 aaaattgata aatgcacggt taacaactcg atttccacct ggtgttgggt aatcgccagt
    42001 gaagtaccaa tcgccaagat tttttggaca ggctctatgt agattttcaa cagtttgaaa
    42061 tataatttta atttcagcct taatatcgtc tgaatgcaat aaagttgcaa ttttatcaga
    42121 aatttgctgg tcagtaaacg gtgcataaat ttcggttacg tgattaataa cctcggaatc
    42181 tttaatgttt tcttgaagtt tacatttttt ataaacttct tcaataatgt ttgttttatt
    42241 attttctttc aataattcta cggcagcttg aaacgcaatt aatccttcta attttgccat
    42301 atcaattcca taacaatctg gataacgtat ttgtggtgca gaagaaacca ctacaattcg
    42361 ttttggacgc aatctgtcca tcatttttag aatactcatt tttaaagttg ttccacgaac
    42421 gatgctatcg tctataataa tcaaattatc tgtgtgtttt attacgccat aggtaacatc
    42481 ataaacgtgt gctaccaaat catcacgatt ggcatcatca gtaataaaag ttctaagttt
    42541 ggcgtcttta atggcgattt tttcagttct aatttttacc gaaaggattt cttctagctt
    42601 ttctttggtt aacgtttttc tattagttag aataaattga ttttttcttt gatttaaaaa
    42661 atcattagca gcttccacca ttccataaaa ggaagtttct gcagtgtttg gaatatatga
    42721 aaaaacagaa ttatccgtat cattttcgat ggcttcaaga actgctggca aaataagttt
    42781 tcccaattct tttcgttcct gataaatctc ggcatcgcta ccacgagaaa aataaatgcg
    42841 ttcaaacgaa catgcttttt ttattgttgg agttatgatt tcttcttctg taacaattcc
    42901 gtttttctta atgattaagg catgacctgg ttgaagttcc tgaactttct caaattctac
    42961 attgaaaacc gtttgaataa ctggtcgttc tgaagcaaca actacaattt catcatcttc
    43021 ataaaaatac gctggacgaa ttccggctgg atcacgaaaa acaaaagcat cgccatgacc
    43081 aagcaaacct gccatcgcat aaccaccatc ccaaccacga gaagcgcgtt ttaatatttt
    43141 tgcaatattt aatttttcgg caataaccgg agaagcttct cttttagata agccattggt
    43201 tttgcattcg tggtataaat cagtaacatc ttcatctaga aaatgaccaa ttttttccat
    43261 aacggttacg gtatcggcta attcttttgg atgttgccct aattctacca aactattgaa
    43321 aagttcggtt acgttagtca tattgaaatt tccagcaaca attaaatttc tatgcatcca
    43381 attgttttga cgtaaaaaag ggtgtacact ttcaatactg ttttttccaa aagtaccata
    43441 gcgaacgtga cctaaaaaca attcgcctat ataaggaatt ttagcttttt gaagcgatat
    43501 gttttctgca tattcgggat gcaaagacat ttcttcatta atacgttcat taatttgggc
    43561 aaaaatatcc tgaataggtt gcgcttgatt ggaacgaacc ctactgatgt atctttcgcc
    43621 tggttcgaca tctagtttta tcgaagcaaa accagcacca tcttgtccac ggttgtgttg
    43681 tttctccatc aagagataca ttttttgaat ggcataaaaa gccgaaccgt atttttgctc
    43741 gtaaaattct aacggttttt ttaatctaag aagtgcaatt ccacattcat gtttaatggc
    43801 atcactcatt gttgtagttg tttagttgta ttgtgtcaaa aaaaatgcct cgacatttcg
    43861 aggcataaag ttattgtaat tctatatcaa attgtgttaa agatttgaat tgtttcaatc
    43921 tgtcgttcac ttcttctttt gtcaaaacaa gcattcgttc agttccaaac ttttctacgc
    43981 aaaacgaagc taagtttgaa ccgtgaataa tagcattttt catattattg aacgaaatgt
    44041 tttcgctttg cgtaataaat ccagcaaaac caccagcaaa tgtatcgcca gcaccagttg
    44101 gatcaaaaac ttcttccaaa ggaagtgcag gagcaaagaa cacatcattt ccatggaaca
    44161 acaaggcacc gtgttcacct tttttaataa caacatattt tggtcccata gtatggattt
    44221 tggcagcagc ttttactaac gaatattcac ccgaaagttg tctggcttct tcgtcattaa
    44281 tggtaatcac atctacgcgt ttcattacgt ctaataattc gggtaaagca caatccatcc
    44341 aaaaattcat cgtgtctaaa accaccaatt ttggtgcttg tgtcatttgg tctaaaaccg
    44401 aactttgtac taatggatgt agatttccta acatcacaac gtcgctattg gtaaaatcgg
    44461 ctggaacaac aggtttgaaa tcagctaaaa cattcaattc cgtaattaga gtgtcgcgag
    44521 agtttaaatc gttatggtat tttccgctcc agaaaaaagt ttttccacct tttacaattt
    44581 ctattccaga aatatcgatg tttctttcag ttaataaatc aaggtattct tgaggaaaat
    44641 catctcctac aactgatacg attgccgaat ttacactaaa atttgaagcc gaaagcccta
    44701 taaaagttcc tgcgccgcca agtattttgt ctgttttgcc aaaaggtgtt tcgatagcgt
    44761 caaaagcaac cgttccaacg attaaaagtt tgttcatttt tgaagtttca aattaaagat
    44821 gcaaaggtaa actttagttt agaagtttaa aagtttatag gtttaaaagt ttaggaatta
    44881 gttgtctttg attttcaagt tcaaccaaaa aagtttttta tttgaactaa aaacattaac
    44941 atctttcatg tttgcaactt cttttatctt tctcatataa gtcatccgca ggtttgtatg
    45001 ttttaagaaa atcttttaaa aaaagtaagg tacaaaattg acttttttgt atctttatac
    45061 catgtatgct ttagtagatt gtaataattt ttatgcttct tgcgaacgtg tttttcgtcc
    45121 agatttgaac ggaaaaccag tcgtaatttt atctaacaat gatggttgtg tgatcgctag
    45181 aagtaatgaa gctaaagcag ttggaattcc aatgggtgcg ccagaattca aataccgaac
    45241 acttttaaaa gaaaataatg taacggtttt ttcgtccaat tacgctttat atggcgattt
    45301 gagtaatcga gtaatgcaga ttttagcaga ttttacaccc aatgtagaaa tttacagcat
    45361 tgacgaagcc tttttaaatt ttgatggaat gacgattgaa gattatcacg attatggtct
    45421 tcaaatgaaa aacagaattc aaaaatgggt tggaatccct acttgtgttg gttttgcgcc
    45481 aacaaaagca ctttctaaaa ttgccaatag aattgcaaaa aaatttgtca ttagaaccaa
    45541 tggtgtttac attattgata cggatgaaag ccgaataaaa gcactgaaat ggacaaaaat
    45601 tgaagatgtt tggggaattg gatatcgatt gtcgaaaaaa gcattgcttc aaaatattaa
    45661 aacggcttat gattttacgt tgccacaaca cgaaaattgg attagaaaac aaatgggcgt
    45721 tgttggcttg cgactaaaac atgaactcga aggaaaatcg gttttagacc ttgaacccga
    45781 aaaaacacag aaaaaaacta tcgccattac aagaagtttt cccaaacaac ttaacgattt
    45841 tgatgcttta aaagaacgta tttcgacctt tgcaaccgtt tgtgccgaaa aattaagagc
    45901 tcaaaaatca agttgttaca ctattattgt aatgcttgtt attgacaaac acaaatacaa
    45961 aaccacaaaa tattatttta atagtgcaat aacattgcca tttgcaaccc atagtagttt
    46021 gacaatttcc aataccgcta ttcgcattct aaaagaatta tatgctggaa acgaacactt
    46081 tacttttaaa aaagcaggtg taattgtaac cgaattgatt ccggaacata atttgcaaat
    46141 caatcttttt gagaatgaaa atccaaaaca ccatatgttg atgaaaacca ttgacaacat
    46201 caatcggaaa atgggtgaaa aaaaagtgaa aatcgccaat caaaacctca acctcacttg
    46261 ggatatgaaa caaaatcatc tttccaacaa gtttaccacg gattttacta aaatattaga
    46321 agtattatgc cactagaaaa aaatcaaaaa ttacagtttt ttattcctga ttttgaaagc
    46381 gatattcaaa ttgcttatag tcacgagatg gtttcggcag gatttccttc accagccaat
    46441 gatttcttag aaactaaaat tgatttaaat aaagagcttt ccaaaaatcc tttggccact
    46501 ttttatgtga aagtaaaagg cgactcgatg ataaattctg gcataaacga caaagatgta
    46561 ttaattgttg accgaagttt ggaacccaat gacaacaaaa tagccgtttg ttttattgac
    46621 ggagaattta cggtaaaacg aataaaacta gacaaaaatt gcctctattt aatgcccgaa
    46681 aacaaaaact accaacccat aaaaattacc gaagacaatc agtttgtcat ttggggaatt
    46741 gtgacttatg tgattaaagc ggtataaaaa cctcaacctt tttagatttt tacccaaaag
    46801 tttagagaaa tttataatta attttgataa taatgagcct gatattatat tgagactcat
    46861 tttgtttaaa tttgactata atctttattt tcacttttta aaaagagtat tttaatgatt
    46921 taaaatgcta ctggtttagc cataaacaaa cactattcta aaaaaatatt ccttaaattt
    46981 gacctataaa aataaatcca aaatgcaaat ccaaggacaa attgttgata tagaaaacaa
    47041 acgcatctat tccggtgaaa tcatcgttca aaacggaaaa atcacatcca taatagaaaa
    47101 acaacacaac aacaaaaaat atatattacc aggatttatt gatgctcaca ttcatatcga
    47161 aagttcgatg ttagtgcctt cggagtttgc aaaaatagcc gtactccacg gcaccgtcgg
    47221 aaccatttcc gatccacacg aaattgctaa tgttttagga aaagaaggcg tgcactacat
    47281 gattgaaaac ggaaaaaaag taccgttgaa gttccatttt ggagcaccat catgcgtacc
    47341 agcaaccttt ttcgaaaccg caggagcagt aatcgattct gagcaaatca aagaactaat
    47401 ggcgatggac gacatctatt acctgtccga aatgatgaat tatccaggcg ttttgtttga
    47461 cgatgaagaa gtactaaaaa aaattgcttg ggcaaaacac ttcaacaaac ccgttgatgg
    47521 tcacgcacca ggattacgtg gcgaacccat aaaaaaatac atctccgcag gtatctcaac
    47581 cgaccacgaa tgtttcacct atgacgaagc cgccgaaaaa ctatcccttg gcatgaaagt
    47641 catcatccgc gaaggcagtg ccgccaaaaa ctttgaagca ttaatcgact tgctgcccga
    47701 acactacgaa aacatgatgt tctgttccga cgacaaacat ccagacgact tgattgtcgg
    47761 gcatatcaat ttgctatgcg cacgtgcagt tgccaaaggg tttgatacat tcaaaatcct
    47821 tcaaatggcg tgcatcaatc cggtgaaaca ctacaacatg aacatcggat tgctaaaaga
    47881 aaacgatgtc gccgacttta tcgtagttga agatttaaaa aacttcaaag tattgcaaac
    47941 ttacatcaac ggcgaactgg tagccagtaa cggacaatcg tttgtaaaac atatcgattt
    48001 cgaaacacca aacaacttca atacctccaa aaaacaagta agtgaattta ctataccaag
    48061 caacgctgca accataagag taatcgaagc tttagaagga caattgatta caaacgaaat
    48121 ccattgcaaa cctaaaatag aaaatggaaa cctcgtttcc gatgtaacaa acgacattct
    48181 aaaaatggcg gttgtcaatc ggtatgccaa tacaaaacca gccgtggcgt tcatcaaaaa
    48241 ctttggatta aaaaaaggtg ccatcgccag ttccgtagct cacgattgtc ataatatagt
    48301 agtagtcgga acatcggacg aagaaatctg caacgctata aatttaatta tcgaaaacac
    48361 cggaggaatt tgtgctataa atggtgaaga acaaaaatca ttggctttac ccgtagctgg
    48421 aatcatgagc aacaaaaacg gttgggaaac cggaaaacta tatcaagaaa tagacgccat
    48481 ggcaaaacaa ttaggaagca cactaaaagc acccttcatg acactttcct tcatggcatt
    48541 actagtaatc cccgatttaa aattatcgga caaaggattg ttcagtggaa aaagcttcgc
    48601 gtttactgat ttggaagtga gttagaaaaa agaccgcttc gctacaagaa gaaagaaaaa
    48661 agatgtttct attattctct tgcttctact ctctttcttc tttattcttt gctcttgcct
    48721 ctgaaacttt cccaccaaca accacaacaa tctccccttt cggaggtttg gcttcaaagt
    48781 gattcagcac ttcttccgca gtaccacgaa ccgtttcttc gtgcagttta gacaattctc
    48841 tagaaacaga aacaggtcgt tccgctccaa agtattgaat atattccgct aaggttttaa
    48901 gtaatttgtg tggcgaaaca taaaaaatca tcgttctagt ttcttccgct aaagccaagt
    48961 agcgagtttg tcttcctttc ttttcgggca aaaaaccttc aaaaacaaaa cggtcgttag
    49021 gcaaaccact attcactaac gcaggcacaa atgccgtagc accaggcaag cattccacag
    49081 ctatattgtt ctcaacgcaa gcacgtgtca acaaaaaccc aggatcagaa atcgcaggcg
    49141 tacccgcatc gctaatcaac gcaatgtttt caccggcttg cagtcgtttg atgaggtttt
    49201 ctacggtttt atgttcgttg tgcatatgat gactgtgcat atgcgtagca atttcaaaat
    49261 gtttcagcaa ttttccactc gttcgagtat cttcagcaag aattaaatcc gcttctttca
    49321 acacttttat cgctctaaaa gtcatgtctt caagattgcc aattggcgta ggaacgatga
    49381 aaagcttacc catagttgct gaatttagtt cagtattatt tgaatttctt ttctacaatt
    49441 tccataaaac gctctaggta ttcttctttt ccaacccaat agttataatc cggaataacc
    49501 atttcgttca agaatgtttt agcttcatca aaagtatcaa aagtgttcaa ctgcgataaa
    49561 actctattat aatcttcgtt actttcatta aacaaatgtt tcacaaaacc aattcggtca
    49621 tttaatccaa tagaaataga atttgataaa gtatcattta aagaaagaac tcttcccgct
    49681 tcttcaacat ttttttcttc aaatactttt acaaatttac cttgaacatt atcttcgggt
    49741 tttacaaaaa caggttcttt aaaatgatcg ccaaaaacat catccatagt aacttgtttt
    49801 gatggtgttt ctttatcaat agtttcttct aaagcctctt tttcttcttc aaaagaaagc
    49861 tcaaaggaag gcataaaact ttcttcttcc gtttcttcaa ctataggttc tgctgatgct
    49921 tcaatttcct cctcaatttc ctcttcttcc tcaataatta tttcgccaac aactacgggt
    49981 tcttcttcta tttttttcgc agtaggaata atagtttctt ccaaaacatc aacttctggt
    50041 tcttttttaa cagctactgc cactggagtt ataacctcat tcgattcaat aaaagcagcc
    50101 actttctctt caatttcttc tacaggaatt gagtttttca acatttcata attatcgcca
    50161 taaaacttta gtatcgtcaa ggcttcgtat aatttttgag tttctttata cagttggtcg
    50221 atttccgatt tgtttttcaa ctttaaaatt cggtgtgcaa tactaattaa ttcggcttca
    50281 actctttttt tcataaggct aaaattatag tgaaataaaa ttgtttttta tatattttgg
    50341 caaaactata ccaaatgctt taataaattt ttctacattt gaaacgctgt aaatgtataa
    50401 aatttatagc gataagtttt attcacaaag taataaaaac tattcatttt aagcacaaga
    50461 attttagaaa atgtttctag agaatacagt aaatcataaa gaacagtttg gttggattga
    50521 agtaatctgc ggttccatgt tttcgggaaa gaccgaagaa ctcatccgta gattaaaacg
    50581 agcacaattt gcaaagcaaa aagtagaaat attcaaacct tcaatagata cacgttatga
    50641 tgaagaaatg gtagtttcgc ataataaaaa tgaaatccgt tccacgccag ttcctgccgc
    50701 agccaacatc cgaattttag cccaaggttg cgatgtcgtt ggtatagacg aagcacagtt
    50761 cttcgatgac gaaattgttt cggtgtgcaa cgatttagcc aattcgggca ttcgagtgat
    50821 tgttgctggt ttagatatgg attttaaagg aaaccctttt ggcccaatgc ctgcactgat
    50881 ggcaacagcc gaatacgtta ccaaagtcca cgccgtttgc acacgcacag gaaatttagc
    50941 caattacagt ttccgaaaaa acgacaatga caaactagta atgctaggcg aaaccgaaga
    51001 atatgaacct ctgagtcgtg cggcctattt taatgccata cgtgaaaata tgattgtgaa
    51061 agatgctgaa catctaaacg acaaagacta aaacaaaacc acattgacaa tagccatccg
    51121 aaatattatt tcaatactca aagcaaattg ggtggccaaa aatgatgtag ccgttatcga
    51181 ttctatttct attgatagtc gctctttaca aaacgattcc aatactttat tttttgctat
    51241 ttctgggcaa aatcacaacg gtcacgaata catagaagat ttaatatcca aaaatgtgga
    51301 gcactttgtg gtcgaataca ttcccgaaaa ccttaaagac aaagccaatt ttctggttgt
    51361 tgaaaacact ttagaagctt ttcaaaaatt tgccacattc taccgaagcc aattcgagtt
    51421 ccccgttatc ggaatcacag gcagcaacgg aaaaaccatt gtcaaagagt ggcttaactt
    51481 ccttttaagt ccagattaca acatcattag aagtcccaaa agttataatt cgcaagtagg
    51541 cgtgccactt tctgttttag gcatcaatga aaaacacaat ctaggaatct ttgaagcagg
    51601 aatttcaatg attaatgaaa tggagaaact acaaaaaatc atccaaccta ctatcggaat
    51661 actaacgaac atcggttctg cccatgatga aggtttttca gatgtaggcg aaaaaattaa
    51721 agaaaagcta aaactttttt caaatgtcaa tgtgcttatt gtcaataaaa acaaaaccat
    51781 atcgtgtttt ataaatccaa aaaccaagca attcacttgg tcgtccgatg ataaaaaagc
    51841 cgatgtattt atcacaactt caaaaaccga gaacaaaacc ataatacagt gcacgtattt
    51901 agaagataca ttccctgtag aaattccgtt tcaagaccaa gcttctatcg aaaatgccat
    51961 tcattgctta gtattaatgt tgtattttgg atacaaccca accgtcatta atgcccgaat
    52021 gagcgaattg tatcctatag aaatgcgcct aaaaataaaa acaggcagta acaattgtac
    52081 tattattgac gacagttata gttctgattt tcaatcctta aaaatcgctt tagactttct
    52141 tgagcatcaa aagcaacaca aaaagaaaac catcattctt tccgatattt ttcaaagtgg
    52201 tctaaccgat gaagaactct attcgcaagt ctctttgcta atcatttcca acaaaatatc
    52261 tcgggtaatt gccattggag aaacgatttc aaaattcaaa cgaaaattta tcaacggaat
    52321 tgcatttgaa aacacaaatg aattcataga tgcttttgat actttagatt tccaaaatga
    52381 aacaattcta atcaaaggcg cacgcgattt tcattttgaa caaattgtta ctttattgga
    52441 acataaaaca cacgaaaccg ttttagaaat caatctaaac gcaataacgc acaatcttaa
    52501 tttctataaa tcaaaactaa atccatccac taaattgatg gttatgataa aagcgtttgg
    52561 ttacggaagc ggtgatttcg aaattgcaaa actgctcgaa catcacaaat taaattatct
    52621 tggcgtggct ttttccgacg aaggaatagc actaaagaac gccggaatca cgattccaat
    52681 aatggtgtta aatccagaat ctaccagttt tgatgctatt atcaatcatc agttagagcc
    52741 agaaatctat tccatcaaag ggttaaaagc atttattaaa attgccgaac taaaaaaaat
    52801 aacgcactat cctattcata taaaaattga cacaggaatg caccgactag ggtttcaaga
    52861 aagtgatttt cccgaactaa tcgaattact taccaataat gagttggtta aaataaaaag
    52921 catcttgtca cacatggcaa caagcgatag tatagaatac aaagattttt cgatttccca
    52981 aattcaattg ttcgataaac tttcttccga attaaaagcg gctttaaaaa taaatccaat
    53041 tcgacatata ttaaacaccg ctggaatttc taattttccc gatgcacaat tcgacatggt
    53101 tcgattgggt attggtctct atggcatttc aagtgatgat gacgaacaaa aatacttgca
    53161 aaacgtagga acattaaaat cgttaatttc tcagatacac accattccaa aaggagatag
    53221 cgtgggttat ggaagacgtt ttattgctaa aagagaaagt aaaattgcca ccattccaat
    53281 tgggtatgct gatggaatct ctagacattt aggaaataat gtgggctatg tcttgataaa
    53341 taataaaaaa gcacccataa tcggcagtgt ttgtatggac atgctgatgg ttgatgcaac
    53401 tgatgttgaa tgcaaagaag gaaataccgt tatcatcttt ggcgaacaac caaccgtaaa
    53461 agaaattgca aaactattaa acacaattcc ttacgaaatt ttgaccagta tttcgcaacg
    53521 tgtaaaacgt attttttata gagaatagtg ttaaatgtta aaaatttaat taatttcgta
    53581 ttactattaa ccaattaaaa acaatcaaat atgggtatga tttcagaatt taaagaattt
    53641 attgcaaaag gaaatgcaat ggatttagca gttggggtta ttattggtgc cgcatttggt
    53701 gctattgtaa actcattagt ttcagatgtg attacaccag ctttattaaa tccagcctta
    53761 aaagcggctc aagttgaaaa tttagcggaa ttaaaaacag atggtggaat tttatacggt
    53821 aaattcttag ctgctgtaat tagttttcta gtaattgctt ttgttatttt tattatggta
    53881 aaaggactta acagcatgaa gaaaaaagaa gaagctgcgc ctgctgcgcc tgctggtcca
    53941 acgcaagaag aattgttaac gcaaattaga gatttattaa aaaagtaatc cgctacaata
    54001 catattctaa cctaaaccgc ttcgtaattg aagcggtttt tctttataat tagtttagat
    54061 attggttttt ttctctatac ttttgcaatt ctaaaaatct attaatcaaa atataaaata
    54121 tgaaagttgc tgttgttggc gcaaccggaa tggttggcga agtaatgcta caagtgttag
    54181 ccgaaagaaa ttttcctgtt acagaattaa ttcctgtagc ctctgaaaaa tcagtaggga
    54241 aagaaattga attcaaagga aaaaaatata ccgtagttgg tcttcaaact gctgtagata
    54301 tgaaagccga tgtagccctt ttttcagctg gcggagaaac ttccttggat tgggcaccaa
    54361 aatttgccgc agcaggaaca accgtaattg ataattcgtc ggcttggaga atggacccaa
    54421 ctaaaaaatt ggtagttcct gaaatcaatg cacacgaatt aaccaaagaa gataaaatca
    54481 ttgctaaccc aaactgttct accattcaaa tggtgatggt gttagcacct ttacataaaa
    54541 aatacaatat caaacgcgtg attgtttcta cttatcagtc cattacagga acaggagtaa
    54601 aagcagtaca acaattagaa aacgaatacg ctggcgtgaa aggcgaaatg gcatacaaat
    54661 accaaattca ccgaaatgct attcctcatt gtgatagctt tgaagaaaac ggatacacca
    54721 aagaggaaat gaaactggtt cgcgaaacac aaaaaatcat tggcgacaaa accattgcag
    54781 ttacagcaac ggctatccgt attccagtgg ttggtggaca cagtgaagcg gttaatgttg
    54841 aattttcaaa cgactttgat gtaaacgaag tacgcactat tcttcacaat accgatggcg
    54901 taaccgttca ggacaactta gatacattca cttacccaat gccgttatac gcacaaggaa
    54961 aagacgatgt atttgtaggt cgtattcgcc gtgacgaaag cagagaaaac gctttaaaca
    55021 tgtggattgt tgccgacaac ctaagaaaag gtgctgcaac taatactatt caaattgctg
    55081 aatacttagt aaaaaacaac ctgatttaat ccgtttttac taaaaatcat aaaagttccg
    55141 cttgatttta cacactaaaa gtatgttaaa gtcgggcgga atttcttttg aaaaaaagta
    55201 cctttgtact gatgaataga aaactactcc ttaccaattt tatcgtagtg tttgcaatgg
    55261 cgtttgcgct gttgtttcag tctattcact cggtggaaca tttacatcaa attgttaccg
    55321 aaaaggcttg tgttcatagc gaaaccaatt caaaaaccaa tttcactcac gaacatcatg
    55381 gtttagataa atgttcgctt tgtgatttta cgttcagcca ttttacatcg ccaataaaag
    55441 ttacgtttga tttttattca tacgaactga atacgccaaa agttgtttcg ctctatgaaa
    55501 cgattaaacc caacttccaa ggaagtttat tttcccatcg cgggccacca ttttttattg
    55561 tttaaacgaa atcttgctgt ccaaatccct tggacaatta ttcatttatc aataaaataa
    55621 ctcatgaaat tcatatatct atacttcata gtaggttgta ctatgatgat aaacgcacag
    55681 aacaaaatct caggaacggt aaccgattct aacaataatc ccattccaag cgctacggtt
    55741 tctttacccg aaattcacaa agaaaccagc accgacaaag acggaaaatt tacactaaac
    55801 aacataccgc aaggaacttt caaactcttg gtgtcgttta tgggttatga aacccaaacg
    55861 caaaccattt ctttatcggg taatgaaatt caactttcca tagtattgac cgaagccatt
    55921 aagcacatgg acgaagtaat tgtttccaat gtattcaagc gtttgcaatc gcaaaacgta
    55981 atgaaggtag aactggaaag tatcaaatca ttacaacaaa aaggaaccgc aacattaata
    56041 gaaggtttgg caaccattcc tggtgtatcg caggtttcta ctggaacttc tattggaaaa
    56101 ccggttattc gtggtttgag tggaaaccgc gtattggttt attcacaagg cgttcgaatg
    56161 gaaaaccaac aatttggcga agaacatggt ttgggactta acgattcagg tgtcgaaagt
    56221 gtagaagtca ttaaaggtcc ggcttcgttg ctctatggtt ccgatgctct gggtggtgta
    56281 ttgtatttca atcccgaaaa atttgccaat ccaaatgatt ttaaagccga tgttagccaa
    56341 cgtttctttt cgaatacttt gggaagcaat acgtcacttg gtttaaaaac ttcaacagac
    56401 aaatggaagt ttttggttcg tggaaactac aacacgcatt ctgattatca aattcctgat
    56461 ggcgaccgag taaccaacac ccgttttaac gaaaaagatt taaaagctgg cattggatac
    56521 aacaacgcaa gttttgcaac cgtgtttcgc tataacttta accaattgga tttaggaatt
    56581 cccgaagatg gtattgcgga gcaatctacc agcaagaaaa caaactttcc tagccaaggt
    56641 gttgacagtc atattttgag tttacacaac aatgtatttt tcaaaaactc aaaactcgat
    56701 attgatttgg gttacatcta caacgaccga aaagaatttg aagacagttt cacgccaatt
    56761 ttgcacatga aattgaaaac tttcaattac actgtgaatt atcatttgcc tagaaaagga
    56821 aagtttgaaa ccattattgg tgcgcaaggt atgaaccaaa ccaacactaa ttttggcgaa
    56881 gaattattaa ttcctgatgc aacaaccaat gattttggga tatatgcaac atcactttac
    56941 gaatggggca aaaacgtact ccaagcagga atccgttacg acaaccgaaa agttgcttcc
    57001 gaaaatcatg gaacaatagg caacgaaggc tattttgaag ccatcgataa atcgtttgac
    57061 agcttcaatg cttcgttagg atataaaacc aacttttctg aaaacctgac catgcgatta
    57121 aatctggcta gtggtttcag agcaccaaac ttggccgaat taacttccaa tggcgttcac
    57181 gaaggaagca atcgctacga aattggaaac agcaatctgg acaacgaaca aaacttccaa
    57241 accgatttga atctagaata caaaagcaca cactttgagt ggttcgtaaa cggtttttac
    57301 aatcatattt ccaattacat tttcatttct ccaagcggaa cacaattgga cggttatgat
    57361 gtgtataatt acgagcaaag caatgcagcg ttgtttggtg gcgaagttgg cgttcacttt
    57421 catccacatc caattgattg gctgcactta accagcagct ttgaaacggt aaccggcaga
    57481 aaagacaacg gcgatgattt accgcttatt cctgccaaca aatggaacaa tacgttgaaa
    57541 gccgaattcg aacaaggaaa ttggttaaaa gatggttttg caaccttaaa tattgtttac
    57601 actttagcac aaaacaattt tagtgaattt gaaaccaaaa ccaacgatta cactttagtg
    57661 aacctaggac ttggcggaga aattacgatt aacaaaagta aattcaatct aaacctaaac
    57721 gctaataatc ttttaaacaa aacctatgtt tcacatctgt cgcgattaaa aaccgatggt
    57781 attcctaata ttggcagaaa tataatgctg ggtattaact tcacaatcta aaacaaaagc
    57841 ggcttgaaat tcaagccgct tttgtattta aaaacggttg tcgccgtcaa tcattcttcc
    57901 caatccacca agtaagcttc cttcttcgcg actattgccg ccattccttg gtgcagaagc
    57961 aataattcta tccgccaaac gactaaacgg taacgattgt acatagacaa ttcctggtcc
    58021 gcgaagtgtg gcaaaaaata atccttcacc gccaaagatg gaatttttaa taccgccgat
    58081 aaattcgata tcgtaatcaa catcttttgt aaacccaaca atgcaacccg tatctacttt
    58141 aagcacttca ccagcagcca attctcttcg agctaatgtt ccgccactgt gtacgaatgc
    58201 cattccgtcg ccttctagtt tttgcatgat gaacccttcg ccgccaaata atccacgacc
    58261 tagttttttt gaaaattcta taccaataga aacccctttt gcagtgcata aaaaagaatc
    58321 tttttggcaa ataaactttc cgccatattt agttaaatca ataggaacaa tttttcctgg
    58381 ataaggcgaa gcaaagtacg ctttcttttt tcctcgttct tgattgatgt atgccgtcat
    58441 aaaaagactt tcgcctgtta gaactctttt tccagccgaa agaagtttat caaaaatgcc
    58501 ttgttgttct cgagaaccat cgccaaaaat agtttgcatt tgtatcccat tttccatcat
    58561 cataaaactt cctgattctg ccacaacgat ttcttgcgga tcaagttcta tttctacata
    58621 ttgcatttct tcgccataaa tgtgataatc gatttcgtgt gctttcataa tttgattgta
    58681 tttaatgatt acttattagt ttacggtttc taataaatgt tacatttaaa tatttattag
    58741 aattttaaac atttgaaaag aggattttta aaaatcatcg ttgaaatttt gttttatatt
    58801 agaaaattaa tgttattttt acattataac caaactaaac ccaacgatta tgaattcaat
    58861 gtttactaaa attgctagaa ttttgctcgg attaatgctt gtcgtttttg gagcaaataa
    58921 attattcact ttatccccat ggatccacca actggtgctg ctggagattt tatgaattca
    58981 cttggtgcta caggatacat ttttccagta gtaggtgtgt tagaaataat tattggatta
    59041 ttgctgttgt taagaaaatg ggttgctttt gctttaatat tgttagcgcc aatttctata
    59101 aacattcttt tatttcactt atttttagat attcctggat tgtcctttgc cttattagtg
    59161 gttgttatta attcaattct aatttataaa cattgggaaa aatatagacc tttgtttaat
    59221 aactaaaata gtattaaaaa agcttcaaaa cctatgtttt gaagcttttt taatttcagt
    59281 ggctatatag aattacctaa acgcttgttc taaatctgct aataaatcat caatgtcttc
    59341 tacaccaacg ctcaaacgca ctaaatcgtc ggttatacca acttccaaac gtttttcttc
    59401 tggtatggaa gcatgtgtca tcaatgttgg atgattagca agtgactcta cgccaccaag
    59461 cgattctgcc aaggtgaaga cttttatttt ttctaaaaat gaaatggcat cttgcatttt
    59521 tccagatttg aatgtaaagg aaaccattcc tccaaaaccg ctcatttgtt ttttggcaat
    59581 gtcatgaaaa ggatgatcgg gcaaaccagg atagaaaacg ttatcaactt cgggatgatt
    59641 gctcaaaaat tcggctactt tcattccgtt ttcgcaatgg cgttgtacac gtaaatgcaa
    59701 agttttgata ccacgtaaaa ctaagaaact gtccattgga cctagtgttg ctccggtggc
    59761 aaattgtttg aagtgcaact cgtcgcctaa tgttttgtct tttataacca atgctccagc
    59821 aataacatca gaatgtccgc caagatattt ggttgccgaa tgcattacga tgtcagcacc
    59881 taaatccaaa ggcttttgta aatatggcgt ggcaaaagta ttatcaacag caaaaagtaa
    59941 tttatgtttt ttagtgattt tagcaatttc ttgaatatcg gctagcttca ttaacggatt
    60001 agtaggagtt tctacccaaa ctaatttagt gttttcatta atcaacgact gaaatttcgc
    60061 taaatcattc atatctacga aatgaaattt gatgccgcta tctttatata aacgagtaaa
    60121 taaacgatag gttccaccat ataaatcgtc catagcaatg atttcatcgc ctgctttaaa
    60181 cattctaagt aaacaatcgg ttgctgctaa accagaggaa aaagccaatc cacgagcacc
    60241 attttctata cttgccaagg catcctctaa agcctgtcga gttgggtttg ctgctcgaga
    60301 atattcataa tcaccaattg gttttcctgg tgacgtttga acataggtag aagtttgaaa
    60361 aacgggtggc attactgctc ctgttacttt ttcatggtgt tgattgccat gtattacttt
    60421 agtgttgaat ttcatattgg aattaatttt gttgtacaaa agtactaagt ttattttgaa
    60481 ttaatttttt tcttttaact actagagtta gaaaacaata tgttttttgc tgactagtgt
    60541 ttattataaa actaagtaaa ctttttataa cgctaataga gtttataata actctaaaac
    60601 agtttatttt aatcataatt taaattattt attttctatt aaagtttatc ttaaacctat
    60661 tataaattat gataaaccta ataaagttaa ttgaaaccct attttattta gatgaatttc
    60721 ttaatttgca tcatgattcc tagatgtaat ccttcgtgat aattgttgaa ttccaaggct
    60781 tctttggcat tcgtcatagt ataacccgtc atggaagtaa attcggaata ggttttaaaa
    60841 attttatttg agaaatctaa ctttgtttgt tctactgttg aaaaaagcaa tgacttcatc
    60901 tcatttattt cttcttgaga aaccatttct tcaggttttg tgccttttct gtattttgct
    60961 accattctat cagaaatcat caatggtaaa cctgatagtt tatagattaa taactgttga
    61021 acaacaatga tgtgtccaat attccagatt aaattattgc tatatccttc tggaattgtg
    61081 tttagtttat caacggaatt atgttctaaa aaattgaata gaactttcct actggttaaa
    61141 agaatttcaa atgtagtttt catttttttt gataaaatta catcatttaa aatttaaatg
    61201 aattttcttt gccaaaagaa acatcatgac taataaaata tattatctcg ctacgtgtag
    61261 cacttgtcga aaaatcataa aatcattgcc taactcggat aagttgattt ttattgatat
    61321 tcgcgaaaac cctataaagg agaaagattt agaagaatta taccaacttt cggggagtta
    61381 tgaatcactt tttagcaaaa aagctcaact ttataaggca atggatttga agaataaaaa
    61441 tttaacagaa caggactata aaaaatacct tttggaacat tacacttttt taagtcgtcc
    61501 ggtttttatt attgacgaca agatttttat tggaaatggt caaaaaaaca tccttgatgt
    61561 tgtaaaagcg ttgagttaat gtcaaaaaga acttgggcat taatcgccgc aactattgtt
    61621 tctattattt acggtatgtc tttcacgatt gcgaaagacg taatgccact gtatataaaa
    61681 ccgtttgggt ttattttaat tcgtgttttt ggagcaactt tgctattttg ggcgatttct
    61741 ttttttggcc caaaagaaaa aattgataaa aaagattttc ctcgaattat cgttgcagcg
    61801 ttatttggag tggcgttaaa tatgctgact tttttcaaag gattgagtta tacttcgcct
    61861 atttctggag cggtgattat ggtaactacg ccaatgattg ttttgatttt atcggcaata
    61921 atcatgaaag aacgaatgct gaaacgaaag gtttttggta ttgttttggg gttaatcggt
    61981 acagggtttt taattcttta tggaaaatcg gttggaaatg cgaccaatgc ttcattggga
    62041 aatttgttgg tatttatcaa tgcggtttcc tatggatttt acctgattat tgtcaaaaaa
    62101 ttgatggata aatacaatgc ttttaccttt gtaaaatgga tttatacgtt tggtttgttg
    62161 atggttattc cttttggata taacgaattt caagaaatag aatggcaatc gataccaaca
    62221 attattcttt gggatatagt ttttgtggtc gtttttacaa cgtttattac gtatttattc
    62281 aatttaattt ccatgcgcga acttaaacca acaacggttg ccgtttttat ttatttacaa
    62341 ccattatttg ctactatttt tgccatcagc ctcggaaaag acgaattgaa tttaattaaa
    62401 atttcagctg cggttttaat ttttgctgga gtttatttgg taacacagaa gaagaatgtt
    62461 cagtaaacag tattcagagt tgcgttttga atacttgcaa aaatacgcaa ctaaaaactt
    62521 ttgagtatat ttgaattcta ttaaaattaa cgaatgatac aatcaatgac tggatttgga
    62581 aaagcatctt tgcaacttcc aaccaaaaaa attaccgttg aactaaaatc tctaaacagt
    62641 aaaggtttag acttaaatac ccgaatgcca tcggttttca gagaaatgga actgggtttg
    62701 cgcaatcaac tatcgcaacg attggaacgt ggaaaaattg atttttcatt gtatatagaa
    62761 gttacaggcg aagaaacttc gtctaaaatc aatgtgccaa ttgtaaaagg ttacattaat
    62821 caaatgaaag ctgttattcc tactgcagat gaaactgaat tgatgaaaat ggctgtaaga
    62881 atgccagatg ctttaaaaac agaacgcgat gaaattgacg aaaatgagtg gaaggaaatt
    62941 caaaaagtaa tcgacgaagc tttagaaaat atagcacaat ttcgaaaaga tgaaggagtt
    63001 tcattagaaa aagaattttt gcatcgaatt gctaatatca tgacattgat gaataatgcg
    63061 gttgcatatg acgttgaacg agttgaaacc gtaaaaacac gtttgcgcac ggctttggat
    63121 gaattaaaag aaaatgtgga cgaaaaccga tttgaacaag aattgatttt ttatcttgaa
    63181 aaatacgaca ttaccgaaga aaaagtacgg ctagaaaatc atttgaacta ttttattgaa
    63241 acattagcag gaactgaagc taacggaaga aaattgggtt ttattaccca agaaatggga
    63301 agagaaatca acactatggg ttcaaaatcc aaccacgctg aaatgcaaaa attagttgtg
    63361 caaatgaaag atgaattgga gaaaatcaag gaacaggttt tgaatgtttt ataagtatca
    63421 agataaaagt attaagtttt aagacgcttt aagctcattt agatttcaaa aacatgaaca
    63481 aaggaaaatt attagtcttc tcagcaccat caggttcagg aaaaacaaca atagtaagac
    63541 atttattgaa acaagaagat ttgaatttag aattttctat ttcagcagca acacgcaaac
    63601 ctcgtggtga agaagtgaat ggaaaagatt attatttcat gtcgattcct gagtttaaaa
    63661 aacacattaa agccgatgat tttattgaat gggaagaagt gtacagagat aatttttatg
    63721 gcactttaaa aaatgaagta gaacgcattt gggcaaaagg gaaaaatgta atttttgaca
    63781 ttgatgttgc tggcggattg cgtataaaat caaaatttcc tcaagaaact ttggcagttt
    63841 ttgtaaaacc accaagtgtt gatgaattga agcgacgttt aaaagaacgt tctaccgaaa
    63901 gtgatgataa aattaatatg cgaattgcaa aagctcatgt tgaattggcc acagcaccac
    63961 aatttgatac tatcataaag aattatgatt tagaaattgc taagcaagaa gcgtataagt
    64021 tggttaagga gtttgtttct tcgtagtaca tagtctttta gtacttagta catagtctgt
    64081 actaaaaaac taagtactaa aaagatatga ttgacactat ttataagtag tttgatagaa
    64141 tatagttttt actaaggact aagtactaaa agactaagta ctaaaaagat atgattgaca
    64201 ctatttataa gtagtttgat agaatatagt ttttactaag gactaagtac taaaagacta
    64261 attactacta aaataaaatt atcacacttc aataataact taataatcac attatgagcg
    64321 gtgtaaaaaa ttataaagaa ttattaattt ggcaaaaagg aattaaacta gtcgttttag
    64381 tttataaact gactagaaat tttccaaaag aagaagtgtt cgctttaact agtcaagtaa
    64441 aaagagcaag tgtttccgtt ccttccaata tcgcagaagg atttggaaga aaaacggaca
    64501 aatcttttaa tcatttttta aatatttcta gaggttcttt aaatgaattg gaaacgcaat
    64561 taatcattgc taaagaactt gaatttattg tagatttcaa cttatatgat gaagttattt
    64621 taatgattga agaagaagca aaaatgataa atgcctttgc aaaaaccatt aaagactaaa
    64681 tttgttaact attaataaaa actatatact tacaaaacta agtactatgt actaaagaac
    64741 tatggactat atatttaaaa actaagtact aaaacaatga aaatcggact ctatttcgga
    64801 acgtttaacc caattcatgt tggacattta atcatagcca atcatatggc ggaacattct
    64861 gatttggatc aaatttggat ggttgtaaca ccgcacaatc cacacaaaaa aaagaataca
    64921 cttttagacg actatcagcg tttgcacatg gtgcatttgg caacagaaga ttttgtaaaa
    64981 attaaaccgt ctgatataga atttaaaatg cctcagccca attatacagt aaacacttta
    65041 gctcatttat tggaaaaata tcctgaacat gagttttctt taattatggg tgaagacaat
    65101 ttgaattcgc ttcacaaatg gaagaactac gaatacatct tgcaacatca tcatattttt
    65161 gtgtatccac gaatgaattc aggagaaata gatgagcaat ttgttaatca tgaaaagatt
    65221 catcgagttg gagcaccagt gattgaaatt tcatccactt ttattcggga aaacattaaa
    65281 aacaaaaaga atgtttcgcc aatgttgcca caaaaagttt gggactattt agaccataat
    65341 ctgttttata cgaagtaaaa taattagaaa ctttttgcaa atcaattagc catctcttaa
    65401 aataaaaaag ccactcatga aaatatttct tgaatggctt ttcccccaaa tcttttgtaa
    65461 aacttactta aaaataaata atgtttctat aaaaacgcaa aaattccttt tttattgtat
    65521 tggaaatcat aaaaataagt taatttttgt agtgtcgtat ttagattatc atcaaactat
    65581 ctttagatat tttttaaaaa gcctattttt ggtattttaa acgaaaccaa aatgaatcaa
    65641 aatccaaaat ttgcagttat cggtggtggc agctgggcta cagcaatcgc aaaaatgctt
    65701 tgtgtaaatt tacccgagat tacttggtat atgcgaaatg aagcatcaat tgatcatata
    65761 aaaacacaaa aacataatcc taattattta agttcggtag agtttgatac caagaaatta
    65821 aagctaactt ctgatattaa tgaagccgtt gcttatgcag attatataat ttttgcaatt
    65881 ccttcggcat ttttaagtgc agaattggct aagttaacag agagcttaaa gggcaaagtc
    65941 attttttcgg caattaaagg aattgtgcca gaaaccttct tgattgttgg tgaacatttc
    66001 aatcaaaaat atgatattcc acttgaaaac ataggtgtaa ttactggacc atgtcatgca
    66061 gaagaagtag cacttgaacg tctttcgtac ttgacaattg cttgtggaga ttcagctaaa
    66121 gccaaagttg tagcaaatac tttagcaagt aattatataa aaactaaaat ttcagacgat
    66181 attattggaa cagaatatgc ggctatgttg aaaaacatat atgctattgc tgctggtatt
    66241 gcacatggtt tgggttatgg tgataatttt cagtcggtga ttatgagtaa tgccattcgc
    66301 gaaatgaaaa aatttatcaa aaaagtgcat aaaatgaagc gcaacattaa taattcggct
    66361 tacttaggcg atttattagt tacgggatat tcggtttttt caagaaatag aatgttcgga
    66421 aacatgattg gaaaaggata tacagtaaaa tcagccatga tggaaatgag catggtagcc
    66481 gaaggatatt atgctgttaa aagtgcttat aagttgaacc aagaatacaa agccgaaacg
    66541 ccaattattg atgctgttta tgaagttttg tatgaaggaa aagatgccag agccactttc
    66601 aaaaaattaa cggataaatt ggattaaaat ctaaaacaat gaataaaata attactatcg
    66661 tttgtgtttt tttagtttct caaaccaatt ttgcacaaga taaaaacacg gcaaaagcgg
    66721 atgttctttt tgaaacttac caatatgttg gtgctataga agaatacttg aaattagcgg
    66781 atactaaaaa tgccacgagt tatgtttatg caaaactagg cgatagttat tacaacattt
    66841 tcaatccaga agaagcagca aaatggtatg caaaagcagt cgaaggaaaa aaagtagacg
    66901 ccgaagtgtt ttttcgttat gctcaaacct taaaatcttt aggaaaatat aaagaagcca
    66961 atagtcaaat ggatgtcttt tcaaaattac ttcctagtga tagtagagca aaagaataca
    67021 aagaaaaccc aaattacatt cccagtttaa gcaatcaaac taaaatgttt gatgtagcag
    67081 aaactacaat tagttataaa aatcaaagtt catttggagc tgttttgacc aatgataacg
    67141 aactttattt tacgagtaca aaaagttcta aaagaacaga taaatctaca gatcaacctt
    67201 atttagatat ttataaagca caacgaaaca ctaacggtaa gtttttggaa gcagaagcag
    67261 taagtgaatt aaatacgccg tatcatgatg gtccggtggc tatcagtagc gatggaaaaa
    67321 caatgttttt tgcacgtgat ggtttaagcg aaggaagtta tcaaaaagac aaaacaggca
    67381 cattaaaagt gggtcaacaa ggaatttata aagcgacaaa agtaggcgat aaatggtcta
    67441 acattgaagc attacccatt aatagcgtga gttattcggt aacacatccc agtttgagcg
    67501 aagatggaag aacattatat tttgcttctg atatgccagg tggaattgga caaacggata
    67561 tttggaaaat ttcaatatct gataatggat atggaaaacc tgaaaattta ggttcaaaag
    67621 taaatactgc cggaaaagaa aatttccctt ttataactgc tgatgaagta ttgtattttg
    67681 gttcgatggg acgacaaggt tttggcggtt tggatgttta taaaatagat ttgaaaacca
    67741 atgaagacgc cgtaaatatc ggaaaaccag tcaatagcga gaaagatgat ttttcgtttt
    67801 cattcaataa aaaagctaat atcggtttct tttcttcaaa tagaaacgga tttgatgcag
    67861 ttttttcggc tacaccaatt tgtaaaacga acgccatcat tatcgtaacc aataagaaaa
    67921 cgggagaaat tatttcaaac gctgaagtaa ctattattga caataaagga aatagtatcg
    67981 aaactcaaaa aacagctgct aaaggaattg tgcaatacaa tgttgaatgt aacacatttt
    68041 attctttaca aatttcagct gctaattatg aaacagcatc tattgtttta gaaaaaagta
    68101 acgaaaaaga aactactaca aaagttcaat tggctccaga agaagtgatt attaccgaaa
    68161 aagaagtgat tttgaaatcg atttattttg attttgataa aagcaatatc acagctcaag
    68221 gcgcaaaaga actagataaa ttagtaaacg tcttgaaaaa atatcctgag atggtaatct
    68281 ttgtaaaatc gcataccgat tcaaaaggaa aagacgatta caatttaaca ctttctgaac
    68341 aacgcgcaca agctactgta caatatgtta tttcaaaagg aattgaaaaa gaacgaatct
    68401 caggaaaagg atttggtgca accgaaccaa aaatcaattg tggaaaaaaa tgtactgacg
    68461 aacaacatgc tcaaaataga cgttcagaat ttttgattgt caaaaaataa ttaccttaca
    68521 ataacaccat caacaaacaa tatcggcact tcttcaacat aatcaggtga aatagattgt
    68581 gctttgaaaa taaatttttt atcgtacaaa gtatttccaa tgaaataagt taccataaat
    68641 tcattattta aacgcaatac acttttttca atcatctcta ttttggcaac tgaaactttt
    68701 ggaatttgag taaatgcgtg acgcaagaac gatgtttgtt tcatttctcc gtcaactgtg
    68761 ccaaaagctt tagaaacgac cattacgcta tctaaatcga agtcagaatc gttaactaga
    68821 tagacatacc aaactttttc cataaagtcg tcgctccatt cttgaacggc agcaagaaaa
    68881 acattttcta caattggaat atggatgtct tttttcattt ttaagatgtt agatttatag
    68941 aaaatagact aacagacgct gtcttttgtc tatcagtctt ttatctttag tctttaaata
    69001 ctggatttaa attgttctaa gaaacgaaca tcgttttcat aaaacatacg aatatcgcca
    69061 atttggtaca ataacatggc aatacgttcg attcccattc caaaagcaaa accagtatat
    69121 tcgtccggat tgatgccgca attttttaaa acatttggat caaccatgcc acaacccata
    69181 atttccaacc aaccagttcc tttggtaata cgataatcgg tttcggtttt taaaccccaa
    69241 tagatatcta cttcagcact tggttctgta aacgggaaat aactaggtct taaacggatt
    69301 tttgactttc cgaacatttc tttggtgaaa tataaaagtg tttgcttcaa atcggcaaac
    69361 gaaacatctt tgtcaatata caaaccttcc acttgatgaa aaatacagtg agaacgcgaa
    69421 gaaaccgctt cgtttctaaa aactcttcct ggagaaatcg ttcgaattgg cggcttgttg
    69481 ttttccatat aacgcacctg aactgatgat gtatgtgtcc ttagcaatac atcaggattc
    69541 gtttggatga aaaaagtatc ttgcatatct cttgccggat gatattctgg taagtttaat
    69601 gccgtaaaat tatgccaatc gtcttcaatt tctggacctt ctgaaa
//
LOCUS       DAOOBH010000003        59019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_622_length_59019_cov_6.269300, whole genome shotgun sequence.
ACCESSION   DAOOBH010000003 DAOOBH010000000
VERSION     DAOOBH010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 59019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59019
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_622_length_59019_cov_6.269300"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2273
                     /locus_tag="PK218_00825"
     CDS             <1..2273
                     /locus_tag="PK218_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPW97085.1"
                     /translation="TLECTQGSTTITKKFTYLFNPGTISTALPSGMLDGINYNTTDAT
                     KATIVLNAPYKDFVYVAGSFNNWTPTSSYAMKKDPITGKFWLEITGLIPGEVYTYQYW
                     VCDQTNRPANSPAVVKTADPFSTLVLSPYDDPEIQALGVYPNLPQYPSGQEREVTVLQ
                     TGPNAWWNYNWSAATTNFVKPKKKDLVIYEVLVRDFDANRTYQDLINKMDYFVNLKIN
                     TIQLMPVMEFEGNLSWGYNTVFHMAPDKRYGPPNKLKEFIDLCHQNGIAVILDVALNH
                     VYGRSPLERMWMLDTDNDGWANNTGYRVSSENPYINQEAMHSYSVGSDLNHFREPDNL
                     TNTYSVRTIQTWIQDYKIDGFRWDLTKGFTNSCPPNVTGGQEACTNGYQSDRVAKLKW
                     YADKQWEFDPNFLIIFEHLGTGGSYNEELEWANYLRSGNNKGILQWRKMTDPYTNVLK
                     GGFGDITAVADVTNRFVGYAESHDEERVVYKAFNETGQTQGNLNKILERLPAMGAIHL
                     LVPGPKMIWHFGELGWDKSLWTCNGGNVSYSNPDCKLDTKPQPQWTENWLENSSRLNV
                     YNSWAKMIDLKKTEDVFENGEYSWNLNVLGSPRLDIWTSNTPTSSLSYVFVRTNFSDS
                     AYTTPGGFPYVGTWYNLMDNSTITVTSTTQNISIEPGGYRIYGNQPSSLSNNNFDFVS
                     NLSLTPNPANNYFTLNEEIEKAEIYSITGQLVKTFQNINTVEYQFNIEDLKSGVYLVK
                     TFDTNAQTKTIKLIKR"
     gene            2385..5150
                     /locus_tag="PK218_00830"
     CDS             2385..5150
                     /locus_tag="PK218_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPW97086.1"
                     /translation="MEKKKKHLLVRILKGTGITIALLLLLMFLLPILFPGKIAQEVKS
                     FANDKLDGELNFKEAKLSFFEHFPSLTLTLTDFSLKGSKPFKKENLISAKDVSFGINL
                     KSLLFDKKVTIDKIFVYDAKVNVLVNEKGEANYNVYISDTKTVDADTSTTSLRLEKIA
                     IKNTHLVYNDKSTKVFIDAKGFNYVGKGDLNESIFDLYTEAKIDSFDFTYDGEQYLKN
                     KKVNANLITQINTNSLAFIFQQNNLKINQLPVEFKGKFNFLNNGYEMDFAIKSEDSYL
                     NDFFTALPPQYVTWLDKTRVKGNTDLALTLKGKYIVSENLKPDLAFNMKIRDGFINHN
                     EAPIPVSNIYLNFDTKLPQLDTEKLQVNIDSIFFNLNKDYVKAIIKSEGLSTPNIDAR
                     IESKVNLAQLNSAFGFGTMDLKGNLNTTIRSKGIYDKKQNKIPVTQGTFVLKNGYIKT
                     DYYPNPIKNINVSATILDKLGNLDDLKINITPASFEFEGKPIYVNAKLENFNNLVYDI
                     KAKGELNIGKIYKVFSQKGLDLEGIVNADVVFKGTQNDAVSGNYTKLQNSGTVRLQNI
                     KTTTEYLPKPFIIREGLFKFHQDKMNFDDFKATYGESDFAMNGALQNVIEFVLTDKAI
                     LKGQFNFSSNYINVDEFMMKSEVVNTNVDTVKKETGVIIIPSNFDLQLKAKAAKINFD
                     GLAINNLNGGLNINKGRLELKKAAVEIIDCKVQLDAVYGNQTDEKAFFDMKVNANDFD
                     VKRAYNEIKMFREIVTAAESAEGIVSLDYKLAGILDGNMMPIYPSLRGNGVLSVSKVK
                     MKGFKLMNVVSRKTGKESIANPDLSKVDIKTSIKNNIITIDRFKFKVAGFRPRIEGTT
                     SFDGRLNIKMRLGLPPLGIIGIPLKITGTQDEPKVKLGKQTEDLQETEYEGEVPAPLL
                     SEEIHQN"
     gene            5280..6182
                     /gene="deoC"
                     /locus_tag="PK218_00835"
     CDS             5280..6182
                     /gene="deoC"
                     /locus_tag="PK218_00835"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPW97087.1"
                     /translation="MKQIDFLNTPRIDQVGIEDRIARITSRSIKKESKMQGLYMALNM
                     IDLTTLEGADTEEKVKQLCFKAHHLHDEIPGLPSTAAVCVYPNFVKTAVNELKGTNVK
                     VAAVATAFPSGQSSAEIKLLDTKIAVDNGAHEVDMVISRGKFHSGEYNFVFDEIAMIK
                     EACGKNVRLKVILETGELGTLDRVRLASDIAMHAGADFIKTSTGKIKPAATLPVTLVM
                     LEAIRDYYYSTGIMIGMKPAGGISTSKLALQYLMMVKETLGGKWLTNEYFRFGASSLA
                     NDVLLQIAKQKSGIYQSNDYFSKA"
     gene            6188..7654
                     /locus_tag="PK218_00840"
     CDS             6188..7654
                     /locus_tag="PK218_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW97088.1"
                     /translation="MKKKHTIDFSSTWDLAPAPESTSHFKLKEQYDLFINGQFLAPKS
                     GKYFDTINPANEKVLAKVAEANEEDVDLAVKAARKAYTNVWSKLSGKERGKYIYRIAR
                     IIQERAREFAVVETLNGGKAIRESRDIDIPLAAAHFFYYAGWADKLDYAFPNRKPKAL
                     GVAGQIIPWNFPLLMAAWKIAPALAAGNTVVIKSSETTPLTLYLLAEVIQEAGLPAGV
                     VNIVSGAGVTGSHIVNHPDVNKIAFTGSTAVGKIIMKAIAGTPKKSTMELGGKAANII
                     FEDAALDQAVEGIVNGIYFNQGHVCCAGSRLYVQESVFDVVVQKLKDRMQSLRVGDPL
                     DKNTDIGAINSKKQLETINKYLKIGVEEGAEIYQPTCDLPEKGYWCRPTLFTKVSQSN
                     RISQEEIFGPVLAIQTFRTVDEVIEKANNTPYGLSAGVWTDKGSKIFNMTSQMRAGVV
                     WANTFNKFDPASPFGGYKESGFGREGGLHGLEPYLKFD"
     gene            7665..8546
                     /locus_tag="PK218_00845"
     CDS             7665..8546
                     /locus_tag="PK218_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW97089.1"
                     /translation="MSRIEVLKTYKIYIGGSFPRTESGRYYPLKSNKKTIANMCLSSV
                     KDFRNAVVAARNAQGSWSHKTAYNRSQILYRIAEVLEGRKTQFIAELELQRVSKKNAE
                     IEVDLSIDRLIYYAGWCDKYVQMYSSVNPVSGSFFNFSVPEPVGVVAVFAPQKSGLLG
                     LVTQIATIISGGNTCVVLASEPLALCAVTFAEVLATSDVSGGVVNILTGNQEELLSVF
                     ASHKDVNSILYCGDDAEKIRKIQDLAIENLKRVVIREDSNYFEDKHESPYLIFDFQET
                     KTTWHPIEVIAGAGAGY"
     gene            8602..9003
                     /locus_tag="PK218_00850"
     CDS             8602..9003
                     /locus_tag="PK218_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97090.1"
                     /translation="MKLFHLFCIVVLGIFLLPSISYACGNVASAKTVKTENISKEPQN
                     SCCKITTKTKKAKSCCDEQQSDSKNKPCEGKCGHSKCSTTSSSSSMTFSSIYQFESKL
                     IDLYSKETNFTFINYNLSSGYVSLWLIPKIS"
     gene            9120..9977
                     /locus_tag="PK218_00855"
     CDS             9120..9977
                     /locus_tag="PK218_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HPW97091.1"
                     /translation="MKNSLKYLMIAFVMVSFISCNAQIKNAKTEQIKIYGNCGMCKKT
                     IEKAGNNKKVSQVDWDKDTKMATITYDAKATNKDEILKRIALAGYDSDSFLAPNDVYE
                     NLPGCCQYDRVAKTPIKEEMKSEMKMEANHSDMTLTEMQKANQLDAVYENYFAIKDAL
                     VKTDGNAASAKANELLISINTLKMSELKIDVHTVWMKVMNDLKADATLIYNSTDAKKQ
                     RASFDTLSENIYQLIKVSKTETPVYYQHCPMANDGKGANWLSKENTIKNPYYGSMMLS
                     CGKVVETIR"
     gene            9978..10538
                     /locus_tag="PK218_00860"
     CDS             9978..10538
                     /locus_tag="PK218_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPW97092.1"
                     /translation="MKLYIKNMVCNRCKMVVKSELEKLGLQLLSVELGEVEILTTISE
                     EQIMRISKKLETFGFEILNDSNSKIIEKIKTILIDLVQNKNNDINITLSDYLIQNLYQ
                     DYSKLSNLFSYVEGISIEKYFINLKIEKVKELIVYDELSLSEIADFLNYSSVSHLSNQ
                     FKKVTGFSPSYFKKIKESKRKELDQL"
     gene            10615..13137
                     /locus_tag="PK218_00865"
     CDS             10615..13137
                     /locus_tag="PK218_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPW97093.1"
                     /translation="MKHTYHIEGMTCSGCEAKVKNELSNVENVTEAEVSKEKKRAIIT
                     MTKHVELDVLQNALGGKDSKYQISLPDSQSQITPKTNCCSTDKKEHKSHEHKHISQPG
                     KYYCPMHCEGDKVYDKAGDCPVCGMDLVKEPTQNVAVQYTCPMHPEIIQDKPGSCPIC
                     RMNLVPMEPTDSEEDKTYQKLWHKMKIAMLFSIPVFIISMSEMIPNNPLFKLMDLEKW
                     NWVQFLLTIPVVFYACWMFFVRAWKSIVNWNLNMFTLIGIGTGVAFIFSIVAMLFPNV
                     FPDQFKTSEGTVHLYFEATTVVLTLVLLGQLLEAKAHSRTSGAIKELLKLAPTEATLV
                     INGEEKVISIHDINKGDIIRVKPGEKIPVDGKITEGNSTIDESMITGEPIPVDKKVED
                     NVSSGTINGTKSFLMVAEKVGAETLLSQIVQMVSDASRSRAPIQNLVDKISRYFVPIV
                     VLISAIAFLIWWQFGPEPKLVYAFVNAIAVLIIACPCALGLATPMSVMVGVGKGAKSG
                     VLIKNAEVLEKMDKIDVLITDKTGTLTEGKPSVEKIVVKDRDENEVLGKIASLNQSSE
                     HPLATAVVNFAKSKIVSFSKVENFESVTGKGVKGSINNELIILGNKKLLEQENSQDFS
                     AIEEQVISEQKQGKTVSYIAIGKKVMGYITITDAIKKSSLEAINELKRQGVQVIMLTG
                     DNENTAKAVASELNLTDFKASCLPQDKLEFIKNLQAKGKIVAMAGDGINDAPALAQSD
                     IGIAMGTGTDVAIESAKITLVKGDLQGIVKAKNLSHAVMKNIKQNLFFAFIYNSLGVP
                     IAAGVLFPVFGILLSPMIAALAMSFSSVSVIANALRLRTIKI"
     gene            13140..15368
                     /locus_tag="PK218_00870"
     CDS             13140..15368
                     /locus_tag="PK218_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HPW97094.1"
                     /translation="MKYLLYSLLSILFIGNLQAQKVVRYDLHVRDSIVNFSGKDKRAI
                     AVNGQIPMPTLTFTEGDIAEIHVYNELKESTSLHWHGLYLPNKEDGVPYLTQMPIEPG
                     TEHVYRFPIIQSGTHWYHSHSGLQEQIGMYGSLILKKKLNDPTFRKGIDDIPQVPIIL
                     SEWTDYKPENVHRMLHNASDWFAIKKGTTQSYTEAIREGHFKTKLTNEWKRMLAMDVS
                     DVYYDKFLINGQNESQLSQFKAGDKVRLHISNGGASSYFWLKYAGGKMTVVGNDGNDV
                     EPVEVDRLIIGVSETYDVIVTIPAENTSYEFLATPEDRTKSTSIYIGEGIKQLTTPMP
                     KLKYFEGMKMMNDMMNMDGTMDDMGMDMSFQKMDMNTVMYPEISGEAMKLDKQEDMKM
                     DHSKHDMSVINSDIVTLNYSMLKSPTKTTLPKDAPVRELRFELTGNMNRYVWSMDNVV
                     LSETDKILIKKGENVRITLYNNSMMRHPMHLHGHDFRVLNGQGEFAPLKNVLDIMPME
                     TDVIEFEANADGDWFFHCHILYHMMAGMNRVFSYENSEPNPYLPNKEKAYKKLQAESN
                     QFHFMAENDFATNGNDGQAMLQNTRWSFGTEWRLGYHDEHGYESETHIGRYIGRNQWF
                     MPFIGFDARYRKLENGEIEENIFGQKSTKDKRIQFSLGFNYTLPMLVIYQTEVFHDGN
                     IRMQLMREDIPVSKRLRMAFMVNTDKEYMAGFKYIVGKNMGFTTHYDSDMGIGFGVNI
                     NY"
     gene            complement(15411..15483)
                     /locus_tag="PK218_00875"
     tRNA            complement(15411..15483)
                     /locus_tag="PK218_00875"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15448..15450),aa:Phe,seq:gaa)
     gene            15579..16307
                     /locus_tag="PK218_00880"
     CDS             15579..16307
                     /locus_tag="PK218_00880"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPW97095.1"
                     /translation="MLLVIDVGNTRIKSAVFEQDKQLGHFVFLKEDLLEKVDFILKQH
                     PKVGTIVVASVGSLEQSDFLAFKSKVKVVFITREFDFPFVNLYESPTTLGIDRMVLAS
                     GAVLQFPKQNRLIIDAGTCITYDFVDETDNYLGGAISPGIRLRYESLHNYTAKLPLLE
                     REFPKSSIGKSTSESIHSGVINSVIFEIDGYIDYFKSENANFIIILTGGDADFLAMQL
                     KNTIFANSNFLLESLNQTFQYNQK"
     gene            16304..17524
                     /locus_tag="PK218_00885"
     CDS             16304..17524
                     /locus_tag="PK218_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97096.1"
                     /translation="MIKKIIVSFGLLISLVSNAQEGTSSPYSFYGIGDVKFKGTVETR
                     SMGGVSVFSDSIHINLQNPAQLAGLKLTTFAVGGSTSNTKLNTETQNEKARRTNLDYL
                     AVGIPAGKFGFTFGLMPYSSVGYKILNLADENNLYSTQYNGIGGINKVFLGLGYKINK
                     NLSVGLDAQYNFGTIETKSFRYLTTIESGTLEENVSRIQGINFTTGLAYEKKLNNKYT
                     YFGSLVYAPQANLKLKNERTITVSDQVDNQPVQNLNFNLPSRLSIGSGFGEVRKWLVG
                     AEVTLQSSSNLKNRFDDINGVVFENSTRYSVGGYYIPNYSSYSSFFKRVTYRGGLRYE
                     NTGMVIQNKSIEDFAVNLGLGLPLSGTFSNLNIGFEVGKRGTKYYNLIEENYINLSIG
                     LSFSDRWFVKRKYN"
     gene            17536..18813
                     /locus_tag="PK218_00890"
     CDS             17536..18813
                     /locus_tag="PK218_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97097.1"
                     /translation="MRVFLLLTVLLFGLNSNAQKKIDCSLQTKEYQEFLSIKNFEEAF
                     GPWEFVAKNCPTQSESLYLEGVKIIQYKIDNAKNEEEKETSVRQLMKLYDQYHKNFPN
                     SIEDYEVYKAMALFDNSIDSKDEVFQLLDNGINSSLNKVTSGNAIFNYFKLLTEKNKN
                     GDKSVTNDKVIEKFTLLNNRIVTLNETNPAEKETYVAASRAINAIADNFLTCDVLTEY
                     YEKKLAQNQENAAWLEASLNVMYEKCAATPIFLTLAEKYYALKVSAQSANYFATALLK
                     NRKFDEAKKYFLESANLEKNTSSKATKYYTLATGIYSNDKPKAKEFLLKSIEFNPKLG
                     KAYLFLAQLYANSASDCAKTDFEKKAVYYLATQTVKKASVADEVLKTNVAMFVNDFSK
                     ESLSESDISNAKMNGKTMTIGCWINEKITFPSK"
     gene            18813..19361
                     /gene="lptC"
                     /locus_tag="PK218_00895"
     CDS             18813..19361
                     /gene="lptC"
                     /locus_tag="PK218_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPW97098.1"
                     /translation="MINKKRTYFFFLLLTFFGMLSCESNFKEVQKMGLSEFSPSGEAD
                     SINLKYTDSGKIKAVLISPKMLDYATAEFPFTEFPVGIDLTLYDEKGKKTYVKSNFAS
                     SYKGTDLIDLQGNVRISNDSGDLLETEQLYFDQKNEWFFTQKKFKFTSPKGVTNGEGI
                     DFSKDFKKVNSQKISGEIDKSE"
     gene            19373..19567
                     /locus_tag="PK218_00900"
     CDS             19373..19567
                     /locus_tag="PK218_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97099.1"
                     /translation="MNYLKFTQYAYLIAGLVFAYDAFTKWQTEDPNYIISAIFAVIGV
                     FMFFFRQRFAKKFEDRNKKP"
     gene            19570..20829
                     /locus_tag="PK218_00905"
     CDS             19570..20829
                     /locus_tag="PK218_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPW97100.1"
                     /translation="MEIGIIILCLILSAFFSGMEIAFVSSNKIYLEIEKKQDDFLGTI
                     LSKITKKPSKFIATMLVGNNISIVIYGFFMGDLILKWFENFGFHFTELSTILLQTIIS
                     TTVILMTAEFLPKVFFQIYANSFMKFFALPAYIFYQLFYYISSFMIWISDFILKKFFK
                     TDGDQINLTFSKVELGNYITEQMNAVNDEDEVDTEIQMFQNALEFAGVKAREIMTPRT
                     EMVAVDLYSSVAELRKLFIETGYSKILVYQNTLDDIVGYIHSFELFKKPRSVKSIMIS
                     VEFVPETIFIKDVMSLLTKKRKSVAIVLDEYGGTSGIVTMEDIVEELFGEIEDEHDQD
                     EALVETQIDESTYMFSTRLDVEYINQEYKLNIPESDSYGTLGGFIVDFASEIPQKGEK
                     ITIGNYQFIIEEATNKKIELVKMTLLD"
     gene            20938..23040
                     /locus_tag="PK218_00910"
     CDS             20938..23040
                     /locus_tag="PK218_00910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW97101.1"
                     /translation="MAVLQKIRNRSGLLLLAIGFALLAFVVQDLFTKGFKSISTDVGS
                     VNGKDISFDEFKIKVANTEKNQQNGQSMTSMQASNQVWNQEVSIALLTAEFEKLGIRV
                     GEKHIVEVFKADPNIGQNQMFLNAAGKFDLAKFKEYFKANPEGIQYLKEREKDADLNA
                     KYQIYNSLVKGGLYTTQAEGKLKYEVETNKVTFDFVNVPFSSIKDSDVKISDDEITAY
                     MKTKEKKFKSEEAREIEYVLIEEKPSAEDEAEIKSKVQSLLNQRVLYNNTIGKNDTIA
                     GFANVKNDAEFVNANSDIPFDSSYVAKQDLPAENAEMLFNLPTGQISAPYMYNGYYCI
                     SKALGRKSGAKSKASHILISWEGTQVPNKKEVRTKEQAKAKAESLLAQAKANPGNFFM
                     LAMMNSDDSSAQSGGDLGYFSPGQMVAPFNDFVFNNPIGKIGLVETQFGYHVIQVTDK
                     QDAVKLATIAQKIQPSEITNNKTYEQATKFEMEAASNKDFDALAKQMKLTVSPSVRVK
                     AVDESFGSISNQRQIVKWAFNSDTSVGDVKRFEIVNKGHVIVKLKKVYDKGLMAVEDA
                     RPQVEPILKNKKKAEMIKAKLKGATLEALATANKVTVMNAVDLTVENPSIPGAGFEPK
                     VVGIAMSSKVGKLSQPVIGNSGVYVLVTKAETKAPAIQNYDEYVNKLKQQVASYSGRV
                     IQALKENADIDDNRADFY"
     gene            complement(23096..24010)
                     /locus_tag="PK218_00915"
     CDS             complement(23096..24010)
                     /locus_tag="PK218_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HPW97102.1"
                     /translation="MSKTFYSNGKLLITGEYVVLDGAMAFALPTKFGQSLVVEESKDK
                     VLNWKSYDYDNSIWFETVIPFSSIIKKERFDITENIKNKLIEILHEAYKINPEFIKHS
                     KGYTVTTALTFPKNWGLGTSSTLINNIADWLQIDAFELLQKSFGGSGYDIACAQNNSA
                     IIYQLEIGKPFSKTIDFNPSFKEKIYFVYLNKKQNSRDAITNYRNKRNEISKLIPEIN
                     SITESVVSTNSIDVFIHQLEKHEMLLSTILEKETAKQLYFPDFNGTIKSLGAWGGDFV
                     MAISNDNPTVYFKSKGFDTILPYQEMIL"
     assembly_gap    24055..24154
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24200..25516)
                     /locus_tag="PK218_00920"
     CDS             complement(24200..25516)
                     /locus_tag="PK218_00920"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HPW97103.1"
                     /translation="MNKEVTGFSKLSKEEKINWIADRYFSNPKESISILKQYWNSDES
                     LQMLHDDFIENTISNFYLPFGIAPNFLINGNYYSVPMAIEESSVVAAASKSAKFWAAR
                     GGFKTTILGTEKIGQVHFLYHGKSEKLEKFFSETKPKFFSNTESLTKNMQKRGGGILD
                     IKLKNKTAELENYYQLHATFETKDSMGANFINSCLEQFAKTLKEEALLYNNFTEDEKD
                     ITVIMSILSNFVPNCVVRAEVSCKVEDLAEKHIQNPREFAEKFIQAVKIAEIEPYRAV
                     THNKGIMNGIDAVVLATGNDFRAVEAGIHAYASRKGTYSSLSHAKIENDIFSFWMEIP
                     LALGTVGGLTSLHPLVKIALEILQNPSAQELMQIVAATGLAQNFAALRSLTTTGIQDG
                     HMKMHINNILNQFQANITERNKVKMHFENHTITHAAVVEYIEKLRN"
     gene            25680..27875
                     /locus_tag="PK218_00925"
     CDS             25680..27875
                     /locus_tag="PK218_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPW97104.1"
                     /translation="MKVIRLLAILLLIATTTVAQQKISVEEIYKGAFRTKGMDELQSM
                     KNTNQYTILNSDRATQSQKIDLYDYATLKQVSTIISTKDFPILSNGIDSYIFSADEKQ
                     LLIANNSVPIYRHSFTADFFLYDIETKKLSKLFEQVQVPTFSPDGLKICYAKENNLFI
                     YEIASKKTTQITSDGLKNAVINGITDWVYEEEFNDPRATGNNIFIRAFDWSLDSKKIA
                     FIRFDESQVPEFTMNIYKKDLYPSNETFKYPKAGEKNSVVSLHVYDVTSKIKKDINLG
                     NYSDFYIARMKWSNDANILSAQILNRHQDNLDLLFIDGTTGSSKIVLNEKDNAYVDVT
                     DNLTFLKDNSFIWTSEKDGYNHIYLYDKTGKLKNQITKGNWEVTAYYGSDEKNKTIYY
                     QSVENGSTIRDVYKISLDGKNKVKLSQKTGTNAATFSPNFEYFINTYSSTIIPTLYTL
                     NNSKDGKVIQTIVDNDVLAKKLETYNLPTKEFFELTTEKGNKLNAWIIKPKDFDASKK
                     YPVFMYQYSGPGSQQVVNEWMNANDYWFMMLTQQGYIVACVDGRGTGFKGAAFKKCTQ
                     NQLGKLEVEDQIDAAKVIGNYPYVDKSRIGIFGWSYGGFMSSNCILKGADVFKMAIAV
                     APVTNWRFYDSIYTERYMQTPQENSSGYDDNSPINHVNKLKGNYLLIHGTADDNVHVQ
                     NSMQMIEALVQANKQFDWAIYPDKNHGIYGGYTRIQLFNKMTNFIKEKL"
     gene            27899..29614
                     /locus_tag="PK218_00930"
     CDS             27899..29614
                     /locus_tag="PK218_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPW97105.1"
                     /translation="MSETTVKAAHPKGLWVLFGTEMWERFNFYGMRAILTLFMVNSLL
                     MREEDASIIYGGFLGLCYLTPMLGGFISDRFFGNRNCIILGGTMMAIGQLMLFFSASI
                     FGSNLGLANTLMWTALGIIVFGNGFFKPNISSMVGSLYPKQEKSKLDTAFTIFYMGIN
                     IGAFLGQFICPFVGDVNNEVLQADGTTLMIRDVHAFKWGFLAAGVAMMIGTAVFFFLK
                     NKYVVTPEGRPIGGLPSGNDNNDYEEGESQKAHFTMGSILTAVVIFAAVFFVFNYFTE
                     GDNIVKTIIYPVIYASGISLAALILLDKTLTKVETQRIWVIYIMSFFIIFFWAAFEQA
                     GSSLTFIADNQTDRHFFGWNMPPSMVQIFNGLFVVMFAFPFSILWDKLRAKNREPLST
                     VKQSIGLLLIAVSYFIIANNVKDLGNTGLLAIKWLILLYLIQTFAELCLSPIGLSLVG
                     KLSPKRFASLAYGVFFISNAAGYALSGTLGSILPATGDKFKKASELGIDLQGVLDKSI
                     TPTAEQLELLAKNQISDHYPTFASFPIENLYDFFMVFVVLCGIAAAILFMLTPVIKKM
                     MHGIR"
     gene            29694..31352
                     /locus_tag="PK218_00935"
     CDS             29694..31352
                     /locus_tag="PK218_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPW97106.1"
                     /translation="MWKSHPKALPFLFLSEMWERFGYYLMIGIFTLYLKDVEAGFAMT
                     EKEASDLYGTFIALVFLTPFIGGLIADRYFGYKKSIIIGGLMMGAGYMMMGIHDKTML
                     YIAMTLVIVGNGFFKPNISTLLGNFYDDEEYKEKKDEGYNIFYMGINVGAFICNFFGA
                     ALQILLGWQYAFMAAGVGMFIGVIVFILGTKHYGDKTEKKGVQPGDMPFYKIVLYILV
                     PSIIFGIIGWLLKGVQSELNPDSYIFGSDSTDAFIFACIPVVYFYASLYFKAKTEDKR
                     PIGALLAIFSVVILFWAVFKLNGSALNTWADRYTDREITGTTEKVFNGLKFSQEVAYQ
                     KDSVSLYDASFRLQKVNGEVQKEYNYPVYFRNVKPEKLPAEGSKVSLWATNLSQSINP
                     GWVIILTPLIVAFFTFLRSRKKEPSTPTKIAFGLLISALSVLVMVAAVEIGNNGSEKV
                     SVWWLVANYGIITIGELFLSPMGLSIVSKLSPKNITSLMMGGWFLATSIGNKLSGVLA
                     SMWDQYDNKANFFWINFGLLLFATVLMFALVKNLNKVMKEKGIN"
     gene            31429..31896
                     /locus_tag="PK218_00940"
     CDS             31429..31896
                     /locus_tag="PK218_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPW97107.1"
                     /translation="MKKIIFAFVFVLGTITMHGQELKWETNLDVAMKISKKTKKPMLL
                     FFTGSDWCGWCIRLQKEVLQTPEFAKWAKKNVVLVELDFPRKTPQDAAIQKQNQELQQ
                     AFQVRGYPTVWFAKASKIDGKVNFEQLGSTGYVAGGPSAWLEGADQILKNKKS"
     gene            complement(31907..33937)
                     /locus_tag="PK218_00945"
     CDS             complement(31907..33937)
                     /locus_tag="PK218_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPW97108.1"
                     /translation="MKILQFPLAKITLWFVFGILLFPSLKTTPPIAFILTVSGFCLVL
                     LCYIFKSKIAFYSSLFGIAVLLNSFFIGLSSASIHKENYSTNHFSKYLNEVENGFNAT
                     ILLTEKLKNTQKNYRYVAEFKSIQKKKVNGKIIFNITKKEKYISLPIGSILFIKGVYY
                     KNKKPFNPNQFDYGNYLEHKEIYGQVYSKTNDVKIIGSQNSLRYYFSNYREKLITNLK
                     KTSLSENSLTVVVALLLGQKHDMSPVIMKEYQAAGAVHILAVSGLHVGIIMYFLLFLL
                     KPMPNTKRANWIKVILIILTLWAFALLAGLSPSIVRSATMFSFLTVGMNIKRTVNIYH
                     TLLVSMLLILLISPSFIYDIGFQLSYLAVFFILLLQPILKSIWKPKNKILNYFWDIIT
                     VSTAAQIGVMPLSIYYFHQFPGLFFLTNMIVLPLLSVIMAIGILVLVLSVIGTVPHFL
                     VQILDLLLKIMNNFIHTIASFDSFTFQNIPFTKEMLWLFYLMIVTLIFWIKKPNFKRI
                     SLALMCVILLQVVFIYQKKEVNKSAEGIVFNQKKGSIISERIGNNVSLFANDSIRKNI
                     HENVTFQSYLVGNFSDLKNKNNIPDLLFINSKKIVVIDSTGIYNPTINPDILIITQSP
                     KLNLERLLLTLKPKVIIADGSNFKTYSKRWEETCRKQKIPFHNTHEKGFYKF"
     gene            complement(33977..34633)
                     /locus_tag="PK218_00950"
     CDS             complement(33977..34633)
                     /locus_tag="PK218_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPW97109.1"
                     /translation="MKYTFKIFFFLFFFISTFGFAQSNKIITTKKEAEKKGIYTAPSV
                     DKAIAYNEPLSKNQKKEIRDKEKKSKKIKAILNTKNAKDIIIDNEETDYVAEQLINNV
                     IDNVGVDYSYGGTSKDGFDCSGLLYSTFKKFDIDIPRVSSSMADIGEKMSIDNAEKGD
                     LIFFATAGGNRITHVGMITEVNGDEILFVHASTSAGVIVSSLSETYYQNTFVQINKIL
                     "
     gene            complement(34688..35800)
                     /gene="lpxB"
                     /locus_tag="PK218_00955"
     CDS             complement(34688..35800)
                     /gene="lpxB"
                     /locus_tag="PK218_00955"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPW97110.1"
                     /translation="MKYYIIAGEASGDLHGSNLMKALFEKDATAEIRFWGGNLMQEVG
                     GTLVKHYKALAFMGFAEVVMNLKTILNNIKFCKKDIEQFKPDVIIFIDYPGFNMRIAK
                     WAKQKGIKTHYYIAPQIWAWKENRIKAVKRDFDKLFVILPFEKDFFEIKHGYPVEFVG
                     HPLIDAIHNRQKTNETQFRKDNHLDDKPVIALLPGSRKQEISKMLEVMLSVTDDFIDY
                     QFVIAGAPSQEFEFYKQFLNTKNVKFISNKTYDLLSISQAALVTSGTATLETALFKVP
                     EVVCYKGSWVSYQIAKRIITLKYISLVNLIMDKEVVTELIQENFNKKRIKVELSKLFE
                     PKNRQEIIRNYEILEKKLGGIGASKKTADLIINDLQ"
     gene            complement(35805..36314)
                     /locus_tag="PK218_00960"
     CDS             complement(35805..36314)
                     /locus_tag="PK218_00960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPW97111.1"
                     /translation="MKKTFSFLLLILTSSITISQEKNSNTEITKIIVVRHAEKIDDGS
                     KNPSLSDIGKLRAKKLDMLFADLKLDALYSTPFSRTTETLQPIANSRNLEIINYSPNN
                     KNFSQNLLLNQKGKTIMIVGHSNTCPDLVNSLINETKFNPLNENEYGKIWIITFKNGQ
                     LIDCVLLNY"
     gene            complement(36332..37105)
                     /gene="surE"
                     /locus_tag="PK218_00965"
     CDS             complement(36332..37105)
                     /gene="surE"
                     /locus_tag="PK218_00965"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPW97112.1"
                     /translation="MMINKPLILVTNDDGITAPGIRTLIEVMSEIGTVVVVAPDSPQS
                     GMGHAITVNNTLYLDKISKDGAEITEYNCSGTPVDCVKLAVHEVLKQKPDLCVSGINH
                     GSNSSINVIYSGTMSAAVEAGIEGIPAIGFSLADYNWNANFEATKPFIKKIALEVLNN
                     KMPNGTVLNVNFPKLNLNEIKGVKICRQAKASWIEKFDKRKSPYGKDYYWLTGEFVND
                     DKGQDTDEYALANGYVSVVPVQFDMTAHHAIQLLNTWDL"
     gene            37268..39442
                     /locus_tag="PK218_00970"
     CDS             37268..39442
                     /locus_tag="PK218_00970"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HPW97113.1"
                     /translation="MKPIFLFMKRNYKVLLVVLALSVAMWSFIPKENTPDPEKDKLLI
                     ELLTFVIEKGHYNPAAIDDTFSKGVYKDYLEALDPSKRFFLQSDIDEFSKYETQIDDQ
                     INNKELTFFDLTYTRLMKRMNDSKAYYEEALSSPIDYKKDETISTDYEKLPYAKNEKE
                     LKDRWRKQVKLSTLSSLVEKEKVQEEKLKSKDNKDAPKTFAELEKETRASTLKSLNDN
                     FGFIKDLNRDDWFSIYINAISSRFDPHTSYFAPNEKERFDVSMSGKLEGIGARLQKEN
                     DFTKITELISGGPAWRGKQLEAGDLILKVAQGNGEAIDVVGMRLDDVVKKIKGPKGTE
                     VKLTIKKPDGTIKVISIIRDEVEIEETYVKSSVVEIDGNKYGIIYLPKFYIDFENKDS
                     RDAGKDVAIEVERLKKEGVQGIVMDVRDNGGGSLKTVVDIAGLFIEQGPIVQIKSAAG
                     KKEVLYDRDAKLQWDGPLVVMINEFSASASEILAAAIQDYKRGVIVGSKQSYGKGTVQ
                     NVIDLNQFVRGSTYGDLGALKTTTQKFYRINGGSTQREGVKSDIVMPDKYEYLKMGEK
                     DVDNAMPWDKIDPAEYKVWTKQHNFDLAIAESKARLANNPQMQLIEDNAKWIDQRSKE
                     NIYSLQMDKFKAEQKALEEKNKKYKPITDYKNDLKFMSLPYEDEMVKNDTVLKEKRDR
                     WHESLSKDVYVDEALHILNDMQNGSDRKGLTDKVKTEKLVKS"
     gene            complement(39515..40027)
                     /locus_tag="PK218_00975"
     CDS             complement(39515..40027)
                     /locus_tag="PK218_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97114.1"
                     /translation="MKLKLLFVAFLAVCYQNNYAQETNSEKVIDSAKVKTATIEAPKA
                     TEKELLEKKIENQKLAIAQKEQAEKAQKQLERDQKKLEKEQKKFQKEQKKIASAEKKL
                     IKYKDKRQDAIDDLEKMKTKFAKNKSKGKLSPVEIEKESLNITKQQLKIKEYEEDIAD
                     YEKKLNKLRN"
     gene            40093..40890
                     /locus_tag="PK218_00980"
     CDS             40093..40890
                     /locus_tag="PK218_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HPW97115.1"
                     /translation="MKNYIVINVFIFLISYAIISCKFLEKDDTTENKKQFSSISAIDL
                     LPTSTTKQIIVHDFYTLSYNEKFEQAEWVAYSLKKENFKQNNFKRPYFVEDKKVTTHS
                     ADWRNYKKSGYDKGHLCPAGDMTFSKKAYEDTFLTSNISPQKHDFNSGIWNRLEQKTR
                     YWAEKYGTVFVISGGVLTSGLKTIGFEKVAVPNYFYKIIASKSNGQIKVIAFLVPNED
                     SKKPLYTFVTSIDAIEKLTNIDFFSKWEDSVENKLESKSDYKDWSFN"
     gene            complement(40978..42240)
                     /gene="rodA"
                     /locus_tag="PK218_00985"
     CDS             complement(40978..42240)
                     /gene="rodA"
                     /locus_tag="PK218_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPW97116.1"
                     /translation="MKNQSVTNNLDWLSIGIYILLVILGWLNIYSSSLSSIEGESSIF
                     DVTQIYGKQFLFILFTIPLIFSIMAIDAKFFEKYSIIIFAVSLLSLAGLFVFGKTIAG
                     QRCWYAIGTFTLQPSEFAKTATALALAKYLSDVQVNLKDMNRQVQAMIILFLPILLIL
                     PQPDPGSALIYSVFFLVLYREGFPSWYLWTGFIAIILFVLALLLEPYVIVIIALVVIL
                     FIHFRSRMIDRNWVLSSIILVLISGYVFSVDYVFDNVFKQHHRDRFNILLGKEVDMKG
                     VGYNTNQSEIAIGSGGLFGKGYLEGTQTKGGFVPEQHTDYIFTTVGEEWGFLGSVLIV
                     ALFLILFGRILYLAERQKTKFSRVYGYCVAGILFIHFFVNISMVIGIFPTIGVPLPFF
                     SYGGSGLWGFTILLFIFLKMDANKVNEW"
     gene            complement(42253..44187)
                     /gene="mrdA"
                     /locus_tag="PK218_00990"
     CDS             complement(42253..44187)
                     /gene="mrdA"
                     /locus_tag="PK218_00990"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPW97117.1"
                     /translation="MRKVLFPTIIIIATILIVSRLFYLQVIDESLKLKSDNNAIKIKY
                     DYPERGYIYDRYGKLMVANQPSYDIMVIPKDVKNIDTLEFCKLLNISKVDFDKKIAKA
                     KVYSPRLPSVFLPQLNKMEYAAFQEMQRKFNGFYIQKRSLRDYQVDCGANVFGFITQV
                     NENIISKNKYYNSGDLIGKQGVEESYEEILRGVKGVKYLQKDKFNRDIGSYKDGKFDT
                     IAVQGKDITLSIDAELQKYGEQLMINKRGGIVAIEPKTGEILALVTSPSYDPAILVGR
                     QRSKNYTMLYHDSIAKPLYDRGLLAEYTPGSPFKILTGLVGLQEGVIDTETSFVCHHG
                     FSYARGRFMRCHDAGVSKLHNGFYNSCNTYFANVYMKTINKYVKPSDGVDTWSNHLKS
                     FGLGQFMGYDLPTGRKGKIPTSKTYKKMYPGWNWDSKTIVSNAIGQGEVLMTPMQLAN
                     MIATVANRGYYYTPHIIKKIKGEMIDKKFRTKHQTTVDKKHFEPVIEGLYDVYNLGTA
                     RGLGVEGIQICGKTGTAENFAKINGKRVKLQDHSIFVAFAPKDNPKIAIAVFVENGYW
                     GARWAGPITTLMIEKYINKKITRKDLEKRMLEGSLQAEYNKYIAKPIVDTLINTTILK
                     SLNTEMKKEEEIKVKDTTKN"
     gene            complement(44184..44690)
                     /locus_tag="PK218_00995"
     CDS             complement(44184..44690)
                     /locus_tag="PK218_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPW97118.1"
                     /translation="MNSTLLVNIARFIFLLLAQVLLFNRIDLFGYINPYPYILFILLF
                     PVNGNKTGLLVASFLLGLIMDMFWNSGGVHATACLVLAYFRPAIFKFSFGLSYEYQTV
                     KLNDVLTPERFSFILIAVVIHHFTLFILEIFKISFIWDIFLRTIFSTIFTIITCILII
                     YIIKPSKR"
     gene            complement(44683..45507)
                     /gene="mreC"
                     /locus_tag="PK218_01000"
     CDS             complement(44683..45507)
                     /gene="mreC"
                     /locus_tag="PK218_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPW97119.1"
                     /translation="MQQIFSFILKNSYRLLFLLLLVISLTLTIQSHSYHKSRVISSAN
                     FLTGGVYQQINNVNEYFGLRKENQELSAENARLKSILFNQKDTTLTPNVNVKGIDKAE
                     IIVSKVIHNSYSTHENFLTLNSGSKDGVKSDMGVINSAGIIGIVDKTSKNYATVISIL
                     NVQSKINAKIKKSNHFGSLVWNGKNTGYAQLIDVPRLASVRKGDTIVTGGQSIIFPEN
                     IGIGVIDKIYTDNVTNYYTLDIKLFNDMTNLGYVYILKTKDRDEVMKLESETIKNE"
     gene            complement(45595..46623)
                     /locus_tag="PK218_01005"
     CDS             complement(45595..46623)
                     /locus_tag="PK218_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPW97120.1"
                     /translation="MGFFDFMTEDIAIDLGTANTLIIHNDKVVIDSPSIVARDRISGK
                     IIAVGKEANMMQGKTHENIKTIRPLKDGVIADFDASEKMISLFIKSIPALKKKLFTPA
                     LRMVVCIPSGITEVEMRAVKESCERVNGKEVYLIHEPMAAAIGIGIDIMQPKGNMIVD
                     IGGGTTEIAVIALGGIVCDKSVKIAGDVFTNDIIYYMRTQHNLFVGEGTAEKIKITIG
                     AATDDLETPPDDMSVQGRDLLTGKPKQVEVSYREIAKALDKSIQRIEDAIMETLSQTP
                     PELAADIYNTGIYLAGGGSMLRGLDKRISQKTDLPVYIAEDPLRAVVRGTGMALKNIP
                     KFRSILIK"
     gene            complement(46678..48204)
                     /gene="purH"
                     /locus_tag="PK218_01010"
     CDS             complement(46678..48204)
                     /gene="purH"
                     /locus_tag="PK218_01010"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPW97121.1"
                     /translation="MSTTKTIQSALISVFSKEGLEPIVQKLHEQNVILYSTGGTEDFI
                     KNLGIPVVPVEDVTSYPSILGGRVKTLHPKVFGGILNRQDHEGDVQQMVEFNIPQIDL
                     VIVDLYPFEKTVASGASEADIIEKIDIGGISLIRAAAKNFKDTVIVASVNEYASLLDI
                     ISSQNGATILEQRKLFATKAFHVSSHYDTAIFNYFNEDETYFKASISEGQILRYGENP
                     HQKGFFFGEFDKMFSKLHGKELSYNNLLDVDAAVNLILEFKNNAPTFSILKHNNACGL
                     ATRNTMKEAYVAALAGDPTSAFGGVLIANGKIDGATAKEISNLFCEVVIAPSFDEEAV
                     AILSEKKNRIILVQHDVELPNSQVRTCLNGLLVQDRNTITDSREDLKVVTTTAPSEQE
                     IEDLIFASKVCKNTKSNTIVFAKNGTLIASGTGQTSRVDALKQAVEKANNFGFDLKGA
                     VMASDAFFPFPDCVELAHNAGITAVIQPGGSIKDELSINYCNENKVAMVFTGTRHFKH
                     "
     gene            48368..49624
                     /locus_tag="PK218_01015"
     CDS             48368..49624
                     /locus_tag="PK218_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW97122.1"
                     /translation="MLLYLRLLKESFLFAMNALRNNKLRTMLSLLGVTIGIFSIIAVL
                     AAVDSLDKKIKGDLGTLDKNTMYLKSQSFGPSDIPRWKRDQFPEVNYEEFQYLKSNLN
                     DVENLCYQYFMFSREAVKNDGITVSSVNVVPVTYEFADIQRMEINNGRFFNEAESNAA
                     AGVIVLGYNIAEGLFDNVDPIGKSVRIYGQNVKVIGVTKKKGMSMGLEDSDDDSVFLP
                     VNFLRRLYGDNSKNFLPVIVIKPEKDVDIDALKGEITQKLRAYRGVKQGEINNFFLDI
                     LSGFTDMIDSFIGTLKMGGWIISGFSLLVGGFGIANIMFVSVKERTNLIGIQKSLGAK
                     NKFILFQFLFEAIILCVIGGIIGLFLVWIIAMVLTDMLDFEFVLSVGNMLLGTSLAAV
                     IGLIAGILPAISASQLDPVEAIRTGM"
     gene            complement(49919..52435)
                     /locus_tag="PK218_01020"
     CDS             complement(49919..52435)
                     /locus_tag="PK218_01020"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPW97123.1"
                     /translation="MESMMIYVPIVMALIGLAFMAIKRSWVLKQDAGDGKMKEISDYI
                     YEGALAFLKAEYKLLTFFVIGASLVLAGISFIVPTTHILIVVAFIFGAIFSAYAGNIG
                     MKIATKTNVRTTQAARTSLPQALKVSFGGGTVMGLGVAGLAVLGLTAFFIIFFRYFMN
                     GVWTSTEDMTIVLETLAGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPED
                     DPRNPATIADNVGDNVGDVAGMGADLFGSYVATVLAAMVLGNYVIKDMGGMITDAFGG
                     IGPILLPMAIAGFGILFSIIGTMLVSISSDDAKEAQVQGALNKGNWVSIVLTAISCYF
                     LVKFMLPELMTMNFYGEGLKEISSMRVFYATIVGLVVGGVISSVTEYYTGLGTKPVLA
                     IVQKSSTGAGTNVIAGLATGMISTFPTVLLFAGAIWASYAFAGFYGVALAASAMMATT
                     AMQLAIDAFGPISDNAGGIAEMSELPKEVRTRTDILDSVGNTTAATGKGFAIASAALT
                     SLALFAAYVTFTGIDGINIFKAPVLAMLFVGGMIPVVFSALAMNSVGKAAMDMVYEVR
                     RQFKEIPGIMEGTGKPEYGKCVEISTKAALREMMLPGILTIGFPIAIVLLGKLVYADN
                     NQLIAEMLGGYMAGVTVSGVLWAVFQNNAGGAWDNAKKSFEAGVEINGEMTYKGSDAH
                     KAAVTGDTVGDPFKDTSGPSMNILIKLTCLIGLVIAPILGSGHSDTAMASCCVVNEKC
                     GTMSVEECTKKGCAEMTECNSMSKEECMEKGCASTTCKFMNPEAAPMTTETSKEVRIE
                     KSNDNGKVTATVTTVTNGKEEVQNFEGTDEEVQAKIDGLK"
     gene            complement(52562..53158)
                     /locus_tag="PK218_01025"
     CDS             complement(52562..53158)
                     /locus_tag="PK218_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HPW97124.1"
                     /translation="MKIALEYLTTKDKTLAKIIENFGVPIIQKREEGFAPLCHIILEQ
                     QVSIASAKACYAKIVNVLETITPTSILGATDEILRNCGVSRQKTIYLKHLAQKVMANK
                     IDFDSFATKEETAIRAELIQLKGMGNWSIDVYLMFCLQLPDIIPFGDIAIKKTFKELY
                     DSQTLQEMEQISNQWKPYRTLASFIVWHHYLKSRNRNY"
     gene            complement(53158..55641)
                     /locus_tag="PK218_01030"
     CDS             complement(53158..55641)
                     /locus_tag="PK218_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPW97125.1"
                     /translation="MKKLFFLFFFSISLTNLISAQTKVSGKVVDNTNQPIPYANIIFK
                     GTNIGVVSNEDGKFYIESDQNYKTLLVSFVGFPTKEVVLTKQVNYNFIITLSEGENLK
                     EVVVFSGKTSKKNNPALDILRKIWERRRKNGLKMFKQYQYNKYEKVEFDMNTIDSAMI
                     KSKLFKGMEFVFKNVDTSRVTGKTYLPIFINESLSEVYGDNIAGKNKEILTANKNSGL
                     GNGDGVNTFIKDLYNDFDIYENYLYFFDKSFTSPLSRTGIDVYNYVLADTAFVDNKWC
                     YNIVFYPRRKGELTFKGDFWVNDTTFAIKKINMAVTKSANINWVKDIYVEQEFDVLND
                     SIFLPTRDYMMTDFALSKKEDSKGIYGKRTTLYQNFKFDIKKPDEFYREEVNFIDNSV
                     YNKSNDFWRQNRFERLNKDEEGIYKMLDTLKTVKKFKRMYNLVSILGSGYIEMNNFDY
                     GPIFSTFGYNEVEGVRLRVGGRTYFGPNDTWRLQGYTAYGFDDNKFKYGLSGKWMIDK
                     KNRFIISGGNRRDVEQIGASLTTTNDVLGRSYASSGLFTTGSNGKLTNINLSTIEAEI
                     EPIKNLSFQIGFSYRTLASASETFSLDYFTDDTFSTIKSEVKQSEINLQLEFTPNRKT
                     IGYGVERREVNSPYSRVFINYSSGFKGLFGSDFDYHKLQLYYRQPLIVGAFGRSNLTM
                     ELGKTFGQIPLGLMSVVPGNQTYFIIKNTFSNLNFYEFITDEYATFQWEHNFNGNIFS
                     RIPYLRKLNLQEIIGIRGVYGTISDENRAINASGLVYVAPEKPYWEYSAGIGNIFKVF
                     RIDCAWRANYRNLPDTNNFTIKGSFGFYF"
     gene            complement(55702..56679)
                     /locus_tag="PK218_01035"
     CDS             complement(55702..56679)
                     /locus_tag="PK218_01035"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HPW97126.1"
                     /translation="MRTIQFREAIAEAMSEEMRRDESIYLMGEEVAEYNGAYKASKGM
                     LDEFGPKRVIDTPIAELGFAGIAVGSAMNGCRPIVEYMTFNFSLVGIDQIINNAAKMR
                     QMSAGQFPMPMVFRGPTASAGQLGATHSQAFENWFANTPGLKVVVPSTPYDAKGLLKA
                     AIRDNDPVIFMESEQMYGDKGEVPDGEYILPLGVADIKREGTDVTIVSFGKIIKEALT
                     AAEELSKEGISCEIIDLRTVRPMDYDAIINSVKKTNRLVVLEEAWPFASVASEITYMV
                     QERAFDYLDAPIQRITTADTPAPYSPTLLKEWLPNAEDVIKAVKKVTYK"
     gene            56908..57654
                     /locus_tag="PK218_01040"
     CDS             56908..57654
                     /locus_tag="PK218_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPW97127.1"
                     /translation="MKILVCISHVPDTTSKINFVNGDTEFDTNGVQYVINPNDEFGLT
                     RAIWFQEQQSANVTVINVGGAETESTLRKALAIGANEAIRINATPTDGFFVAKQLAEV
                     AKNGNYDLIICGKESLDYNGGMVPGMLATLLNANFVNSCIDLKVDGNSAKVIREIDGG
                     KETLSTSLPLIIGGQKGLVEEKDLRIPNMRGIMTARTKALTVLEPITSDNKTKSVKFE
                     KPASKSAVKLISPDNLDELINLLHNEAKVL"
     gene            57764..58732
                     /locus_tag="PK218_01045"
     CDS             57764..58732
                     /locus_tag="PK218_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPW97128.1"
                     /translation="MSLLIYAESAGGKFKKVAFELASYAKKVAETLGTTVTAVTVNAN
                     DVSELSKYGVDKVLKVSNDKLTNFNAKAYADIVKQAAQKEGAKVILLSSTTDSLYMAP
                     IVAVGLEAGFASNVVGLPLSTSPFQVKRNAFSNKAFAITEIESEVKVLGLAKNSYGIV
                     ESASSLTEEDFSPSLNEADFNIKVDSVDKSSGKISIADAEIVVSGGRGLKGPENWGMI
                     EELASVLGAATACSKPVSDLDWRPHSEHVGQTGKPVAANLYIAIGISGAIQHIAGINS
                     SKVKVVINTDPEAPFFKVADYGIVGDAFDVVPRLTQKLKEFKANNS"
BASE COUNT        20485 a         8934 c        10012 g        19488 t
ORIGIN      
        1 ttactttaga gtgtactcaa ggatcaacaa caataactaa aaaatttaca tatcttttta
       61 acccaggcac tatttcaaca gcacttccat ctggaatgtt ggatggaata aattacaata
      121 caactgatgc caccaaagcc acaatagtat taaatgctcc ttacaaggat tttgtttatg
      181 ttgcgggaag ttttaataat tggacaccaa cttcttctta tgctatgaaa aaagacccca
      241 taactggaaa attttggttg gagattacag gtttaattcc aggagaagtt tatacttatc
      301 aatattgggt ttgtgatcaa acaaatagac ctgctaactc cccagcagtt gtaaaaacag
      361 ctgacccatt ctctacatta gttttatctc cttatgatga tcctgaaatt caagcattag
      421 gagtatatcc aaatttacct caatatccat caggtcaaga gcgtgaagta acagttttgc
      481 aaactggccc aaatgcttgg tggaattata attggagtgc tgcaacaact aattttgtta
      541 aacctaaaaa gaaagacttg gttatttatg aagttctagt aagagatttt gatgcgaata
      601 ggacttatca agatttaatt aataagatgg attattttgt taatttaaaa ataaatacta
      661 ttcaattaat gcccgttatg gagtttgaag gtaatttaag ttggggttat aatacagttt
      721 tccatatggc acccgataaa agatatggac ctccaaataa gttgaaagag tttattgatc
      781 tttgtcatca aaatggtatc gctgtaatat tagatgttgc gctaaatcat gtttatggtc
      841 gttctccttt agaaagaatg tggatgctag atacggataa tgatggttgg gcgaataata
      901 ctggatatag agtttcttct gaaaatccat acatcaatca agaagctatg cactcatata
      961 gtgtaggtag tgatttaaat cactttagag agcctgataa tttaactaat acttattctg
     1021 ttagaacaat tcaaacttgg atacaagatt ataaaattga tggttttaga tgggacttaa
     1081 caaaaggatt tacaaattcg tgcccaccaa atgtaacggg aggtcaagaa gcttgtacta
     1141 acggttatca atctgataga gttgcaaagt taaaatggta tgcagataaa caatgggaat
     1201 ttgatccaaa tttcctaatt atttttgaac atctcggaac tggagggtct tataatgaag
     1261 agttagaatg ggcaaattat cttagatctg gaaataataa aggaatacta caatggcgta
     1321 agatgacaga tccttatact aatgttttaa aaggcgggtt cggtgatatt actgctgtag
     1381 cagatgtaac aaacaggttt gttggttatg cagaaagtca tgatgaagaa agagtagtat
     1441 ataaagcatt taatgaaact ggacaaacac aaggcaattt aaacaagatt ctagaaagac
     1501 ttcctgcaat gggtgcaatt cacttgctag taccaggacc aaaaatgatt tggcattttg
     1561 gagaattagg ttgggataaa tctctatgga cttgtaatgg tggtaatgtg tcgtattcta
     1621 atccagattg taaactagac actaaaccac agcctcaatg gactgaaaat tggcttgaaa
     1681 attcttctag attaaatgtt tataacagtt gggctaaaat gatagatctc aaaaaaactg
     1741 aagatgtttt tgaaaatggt gaatactctt ggaatttaaa tgttcttgga agtcctaggc
     1801 tagatatttg gacaagcaat acaccaacat cttctttaag ttatgttttt gtgagaacaa
     1861 atttttctga tagcgcatac acaactccgg gaggttttcc atatgttgga acttggtata
     1921 atttaatgga taattccaca ataacagtaa catctaccac ccaaaacatt tctatcgaac
     1981 caggtggata tagaatatat ggtaatcagc catcgtcatt aagtaataat aatttcgatt
     2041 ttgtttcaaa tttatcatta acaccaaatc ctgccaacaa ttattttact ttgaacgaag
     2101 aaatagaaaa agcagaaatt tattcgataa ccggacaatt agtgaaaaca ttccaaaata
     2161 taaacacggt tgaataccaa ttcaatattg aagatttgaa atcaggagtt tatttagtaa
     2221 aaacttttga tacaaatgct caaacaaaaa caattaaatt aattaaacga taaggttaga
     2281 gttagtattt aatttgaaca agctgcgtag tttttacgtg gcttgtttgc ttttataaga
     2341 tctttgaaat tgattgaata agaatagtat atttgcaaaa tattatggaa aagaaaaaaa
     2401 agcatttact cgttagaatc cttaaaggta ctggaattac aattgcgctg cttttgttgt
     2461 tgatgttttt attgccaata ctttttcctg gtaaaattgc tcaagaagta aaatcgtttg
     2521 ccaatgataa attggatggt gaattgaatt ttaaggaagc aaaattgtct ttcttcgaac
     2581 attttccgtc attaactctt acgcttacgg atttctcttt gaaaggttct aagcctttta
     2641 aaaaggaaaa cctaatttct gcaaaagatg tttcatttgg tattaactta aagtcattgt
     2701 tgtttgataa aaaagttact atcgataaaa tctttgttta tgatgcgaaa gttaatgtat
     2761 tagtaaatga aaaaggagaa gccaattata acgtttatat ttcagatact aaaacagttg
     2821 atgccgatac ttctacaact tcattacgtt tagaaaaaat agctattaaa aatacccatt
     2881 tggtttataa tgataaatct acaaaagttt ttattgacgc caaaggtttt aattatgttg
     2941 ggaaaggcga tttgaatgaa tcgatttttg atttatatac tgaagccaaa attgactctt
     3001 ttgattttac atacgatggc gagcaatatt taaagaacaa gaaagttaat gccaatttaa
     3061 ttacgcaaat caacaccaat tccttagcat ttatatttca gcaaaataat ttaaaaatta
     3121 accaacttcc tgttgagttt aaaggaaaat tcaatttctt gaataatgga tatgaaatgg
     3181 attttgctat caaatctgaa gacagttatt tgaatgactt ttttacagca ttaccaccac
     3241 aatatgtaac gtggttggat aaaacgagag taaaaggaaa tacagatttg gctttgactt
     3301 taaaaggaaa atatattgtt tctgagaact taaaacccga tttagcgttt aacatgaaaa
     3361 tacgtgatgg gtttattaat cataatgagg caccaattcc agtttccaat atatatttga
     3421 attttgatac taaattgcct caacttgata ctgaaaagct acaagttaat atagattcta
     3481 ttttctttaa tctcaataaa gattatgtaa aagctatcat aaaatcagaa ggtttatcaa
     3541 caccaaatat tgatgcacga attgaatcaa aagtaaattt ggcgcaattg aatagtgctt
     3601 ttgggtttgg tactatggat ttgaaaggaa atttgaatac cactattcgt tccaaaggca
     3661 tttatgataa aaaacaaaac aaaataccag taacccaagg aacatttgtt ttaaaaaatg
     3721 gctatatcaa aactgattat tatccaaatc cgataaaaaa catcaatgta tccgcaacta
     3781 ttcttgataa attaggcaac cttgacgact tgaaaataaa cattacacca gcaagttttg
     3841 aatttgaagg aaaaccgatt tatgtaaatg caaaactaga gaatttcaat aatttagttt
     3901 atgatataaa agcaaaaggc gaattgaata ttggaaaaat ttataaagtt ttctcccaaa
     3961 aaggattgga tttagaagga attgttaatg ctgatgtagt ttttaaagga actcaaaacg
     4021 atgctgtttc aggaaattat accaaacttc aaaacagtgg aacagtacgt ttacaaaata
     4081 taaaaaccac aaccgaatac ttgccaaaac ctttcattat tcgtgaagga ttgttcaaat
     4141 tccatcaaga caagatgaat tttgatgatt tcaaagctac ctatggcgaa tctgattttg
     4201 ctatgaatgg tgcgttacaa aatgtgattg aattcgtttt aaccgacaaa gccattttaa
     4261 aaggacaatt taatttcagt tccaattata tcaacgttga tgagtttatg atgaaatcag
     4321 aggttgtaaa cacaaatgtt gatactgtaa aaaaagaaac tggcgttatc attattcctt
     4381 ctaattttga tttacaactg aaagctaaag cagctaagat taactttgat ggattagcga
     4441 taaataattt gaatggtggt ttaaatatca ataaaggcag attagaatta aaaaaagcag
     4501 ccgttgaaat cattgattgc aaagtacaat tggatgcagt ttatggcaat caaactgatg
     4561 aaaaagcatt ttttgacatg aaagtaaatg caaatgactt tgatgtaaaa cgcgcttaca
     4621 acgaaataaa aatgttccgt gaaattgtaa ctgctgccga aagtgccgaa ggaattgttt
     4681 ctttagatta caaattagcg ggtattcttg atggaaatat gatgcccatt tatccttcgt
     4741 tgagaggaaa cggagtttta tcggtgagta aagtaaaaat gaaaggtttt aaattgatga
     4801 atgtagtgag cagaaaaacg ggtaaagaat cgattgcaaa tcccgatttg agtaaagtag
     4861 atattaaaac ttcaattaaa aataacatca tcaccattga ccgatttaag tttaaagtgg
     4921 ctggattcag accaagaata gaaggaacaa ccagttttga cggaagattg aatatcaaaa
     4981 tgcgattggg attgccacca ttaggaatta ttggaatacc attaaaaata acaggcacac
     5041 aagacgaacc aaaagtaaaa ttgggtaaac aaaccgaaga tttacaagaa acggaatacg
     5101 aaggtgaagt acctgcgccg ttgttgtctg aagaaattca tcaaaactaa aattatcttc
     5161 ttttattgct tcaaatgtta cgataaaatt gtttaacgtt acttcttgtt gaacttattt
     5221 atatatttgt tattcctatt gttgaaaatt ttaccatagg ttactaattt ttttattaaa
     5281 tgaaacaaat tgatttttta aataccccaa gaatagatca agttggtatt gaagaccgaa
     5341 tcgcaaggat aacgtcgcgt agtatcaaga aagaatccaa aatgcaaggt ttgtatatgg
     5401 cattgaacat gattgattta actactttgg aaggtgcaga taccgaagaa aaagtgaaac
     5461 aattgtgttt taaagcgcat catttgcatg atgaaatacc aggtttacct tcaactgcag
     5521 ccgtttgtgt atatccaaat tttgtaaaaa cagcagttaa cgaactaaag ggaacgaatg
     5581 taaaagtggc agctgtggct actgcttttc caagcggaca atcgagtgct gaaatcaagc
     5641 ttttagacac aaaaatagcg gtagataatg gtgcacacga agtagatatg gtcatcagcc
     5701 gtggaaaatt tcatagcgga gaatataatt ttgtatttga cgaaattgct atgattaaag
     5761 aagcatgtgg caaaaatgtt cgtttgaaag tgattttgga aacaggtgaa ttaggaacgt
     5821 tagacagagt tcgattggca agtgatattg ctatgcatgc tggagccgat ttcattaaaa
     5881 cttcaacggg aaaaatcaaa ccagcggcaa cattacctgt aactttagtg atgctcgaag
     5941 ccattcgcga ttattattac agcactggaa ttatgattgg aatgaaacct gcaggtggca
     6001 tttcaacttc aaaattggca ttacaatatt taatgatggt caaagaaacg cttggtggaa
     6061 agtggttgac caacgaatat ttcagatttg gagcgagtag tttggccaat gatgttttac
     6121 ttcaaatagc aaaacaaaaa agtggtattt atcaatccaa tgattatttc tctaaagcat
     6181 aattcaaatg aagaaaaaac atactataga ttttagttca acctgggatt tagctccagc
     6241 tccagaaagc acatcgcatt ttaaattgaa agaacaatac gatttgttca tcaacggaca
     6301 atttttagca ccaaaatctg gaaagtactt tgacacgatt aatcctgcaa acgaaaaagt
     6361 tttggcaaaa gtagctgaag ccaatgaaga agatgttgat ttagcagtaa aagcagctcg
     6421 aaaagcatac acaaacgttt ggtcaaaatt atcaggaaaa gaacgcggaa aatacattta
     6481 tagaattgct cgaataatcc aagaaagagc tcgagaattt gccgtagtag aaaccttaaa
     6541 cggtggaaaa gccatcagag aatctcgcga tattgatatt ccattggcag cagcacattt
     6601 tttctattat gctggttggg ctgataaatt agactacgct tttccaaata gaaaaccaaa
     6661 agctcttggt gttgcagggc aaattattcc ttggaatttc ccattactaa tggcagcttg
     6721 gaaaattgct cctgcgttag cagcaggaaa tacagttgtt attaaatcgt cagaaacgac
     6781 accgttgaca ttatatttat tagcagaagt gattcaagaa gcaggtttac ccgcaggagt
     6841 tgtaaatata gtttctggcg caggtgttac gggttcacac atcgtaaatc atcctgatgt
     6901 caataaaatt gcctttacag gttctacagc ggttggaaaa ataatcatga aagcgatagc
     6961 tggtacacca aaaaaatcaa cgatggaatt gggtggaaaa gctgccaata tcatttttga
     7021 agatgcggct ttagaccaag cagtagaggg aattgtgaac ggaatttatt tcaatcaagg
     7081 acatgtgtgt tgtgcgggtt cacgattgta tgtgcaagaa tcagtttttg atgttgtagt
     7141 tcaaaaatta aaagatagaa tgcaatcgtt gcgcgtaggc gatccattag ataaaaacac
     7201 cgatattgga gcaatcaatt ctaaaaaaca attagaaacc atcaataaat atttaaaaat
     7261 tggagtagaa gaaggtgcag aaatatacca acccacttgc gatttaccag agaaaggcta
     7321 ttggtgtcga ccaacgttgt ttaccaaagt aagtcaatca aacagaattt ctcaagagga
     7381 aattttcggt ccagttttag caattcaaac ctttagaacc gttgatgaag tcatcgaaaa
     7441 agcaaataac acgccttatg gattgtcggc tggagtttgg acagataaag gttcaaaaat
     7501 attcaacatg acctcgcaaa tgcgcgctgg tgtggtttgg gcaaatacgt tcaacaagtt
     7561 tgatcccgct tcgccatttg gtggttacaa agaaagtggt ttcggaagag aaggcggact
     7621 tcacggttta gaaccttatt taaaatttga ttaatacgga aattatgtca agaatagaag
     7681 ttttaaaaac atacaaaata tacataggag gaagttttcc tagaacagaa tctggaagat
     7741 attatccatt aaagtcgaac aagaaaacaa tagcaaatat gtgtttatcg tcagtgaaag
     7801 attttagaaa tgcagtggtt gcagctcgaa atgctcaagg ctcttggtct cacaaaacgg
     7861 cttataatcg ttcacaaata ttatatcgca ttgcagaagt tttagaagga agaaaaacac
     7921 aattcattgc agaattagaa ttgcaaaggg tttctaagaa aaatgcagaa attgaagttg
     7981 atttaagtat cgatagatta atttattacg ccggttggtg tgataaatat gtgcaaatgt
     8041 atagtagtgt gaatccggtt tctggaagtt tctttaactt ttctgtgcca gaaccagttg
     8101 gtgtcgtggc agtttttgct cctcaaaaaa gtggcttact tggattagta actcaaattg
     8161 caacgataat ttctggtgga aatacttgtg tagtgttagc ttccgaacca ttagcgttgt
     8221 gtgcagttac atttgcagaa gtattggcta cttcagatgt ttctggtgga gtagttaata
     8281 ttcttactgg gaatcaagag gagttattgt ctgtttttgc ttctcataaa gatgtaaatt
     8341 ctattctgta ttgtggagat gatgccgaga agattagaaa aattcaagat ttagctattg
     8401 aaaatttaaa aagagtagtc attagagaag attcaaatta ttttgaggac aaacatgaat
     8461 cgccttattt gatttttgat ttccaagaaa cgaaaaccac ttggcatcct attgaagtta
     8521 ttgctggcgc tggtgctgga tattaactgt tttatagttt aattaacgta aatggtttac
     8581 ttaataatta actttgcctc tatgaagtta ttccacttat tttgcatagt tgttttaggc
     8641 atttttcttt tgccttcaat ttcttatgct tgtggaaatg tagctagtgc aaagaccgtg
     8701 aaaacggaaa acatttccaa agaacctcaa aattcttgtt gcaaaattac tactaaaacc
     8761 aagaaagcaa aatcatgttg tgatgaacaa caatcagata gcaagaataa accttgtgag
     8821 ggtaaatgtg gtcattcaaa atgttctaca acttcttcat caagcagtat gacattttcg
     8881 agcatctatc aatttgaaag taaattaatt gatttgtatt caaaagaaac aaatttcaca
     8941 tttattaatt ataacctatc ttcaggttat gtttccttgt ggcttatccc taaaataagc
     9001 taaattataa tttttgctag ccataattat ggctttttct cagcgactat atgttgttga
     9061 aactcgttta aacttttaaa aaataacaga aacgattcgt ttcaaaaaaa tctcttacaa
     9121 tgaaaaactc attaaaatat ttaatgatag catttgttat ggtatcattt atttcatgca
     9181 atgcacaaat aaaaaatgct aaaaccgaac aaataaaaat ttatggcaat tgtggtatgt
     9241 gcaaaaaaac aattgaaaaa gctggaaata ataaaaaagt atcacaggtt gattgggata
     9301 aagacactaa aatggcaaca ataacttatg atgctaaagc aactaacaaa gacgaaatct
     9361 taaagagaat tgcattagca ggttatgaca gtgattcttt tttagctcca aatgatgttt
     9421 atgaaaatct tcctggttgt tgtcaatacg atagagttgc aaaaactcca ataaaagaag
     9481 aaatgaaatc ggaaatgaaa atggaagcaa atcattctga tatgacttta acagaaatgc
     9541 aaaaagcaaa tcaattggat gcagtttatg aaaattattt tgcaattaaa gatgcattag
     9601 taaaaacaga tggaaatgct gcttctgcaa aggcaaatga attactgatt tcaattaata
     9661 cattaaaaat gagcgaatta aaaatagatg ttcacacggt atggatgaaa gtaatgaatg
     9721 atttaaaagc tgatgcaact ttaatttata atagtactga cgcgaaaaag caaagagctt
     9781 catttgatac actttcagaa aacatatatc aattaattaa agtttcaaaa actgaaacac
     9841 cagtttatta ccaacattgt ccaatggcaa atgatggaaa aggagcgaac tggttgagca
     9901 aagaaaatac tattaaaaat ccatattatg gctcaatgat gttatcttgt ggtaaagtag
     9961 ttgaaactat aagataaatg aaactttata ttaaaaacat ggtttgtaat cgctgcaaga
    10021 tggtggtgaa gtctgagttg gaaaaactcg gacttcaact tctttcagtt gaattaggag
    10081 aagttgaaat acttacaaca atttcggaag aacaaataat gagaatatct aaaaaacttg
    10141 aaacatttgg ttttgaaata ttgaatgatt caaatagcaa aatcattgaa aaaattaaaa
    10201 ccatattaat tgatttagtt caaaataaga ataatgatat taatatcact ttgtctgact
    10261 atttgataca aaatctttat caagattata gcaagttaag taacctattt tcttatgtag
    10321 aaggaattag tatcgaaaaa tattttatca atttaaaaat tgaaaaagta aaggaactaa
    10381 ttgtatatga tgagttatca ctttctgaaa ttgctgattt tttgaattat agtagtgtat
    10441 cgcatttaag taatcagttt aaaaaagtaa caggattttc acctagttat ttcaagaaaa
    10501 ttaaagaaag taaaagaaaa gaactagacc aattataaat tttgcaaatc ctatacataa
    10561 ttatgcaacc ttttaaagta agattactcg aactttgtaa aaagaaattt aatcatgaaa
    10621 cacacttacc atattgaagg aatgacttgc tcaggttgtg aagccaaagt aaaaaatgag
    10681 ttgtctaatg ttgaaaatgt tacagaagct gaagtttcga aagaaaagaa aagagctata
    10741 ataaccatga caaaacatgt agaacttgat gttttacaaa atgctttagg aggaaaagac
    10801 agcaaatacc aaatttcgtt gcctgattct caatcacaaa tcacaccaaa aaccaattgt
    10861 tgttcaacag ataaaaaaga acacaaatca cacgaacaca aacatatcag tcaacccgga
    10921 aaatactatt gtccgatgca ttgcgaaggc gacaaagtat atgacaaagc tggtgattgt
    10981 cctgtttgcg gaatggattt agtaaaagaa ccaacgcaaa atgttgctgt tcaatatact
    11041 tgcccaatgc atcctgaaat aattcaagat aaaccgggaa gttgtcctat ttgtagaatg
    11101 aatttggtgc caatggaacc aaccgattcc gaagaagaca aaacctatca aaaattgtgg
    11161 cacaaaatga aaattgcgat gctttttagt attcctgttt ttattatttc catgtccgaa
    11221 atgattccaa ataatccttt atttaaattg atggatttag aaaaatggaa ttgggtgcaa
    11281 tttctcttaa ctattcctgt ggtgttttac gcttgttgga tgttttttgt aagagcttgg
    11341 aaatcgattg taaattggaa tttaaacatg tttactttaa ttggaatcgg tacaggagtt
    11401 gcttttatct tcagtattgt tgccatgttg tttccaaatg tatttcctga ccaattcaaa
    11461 acttcggaag gtactgttca tttgtatttt gaagcaacaa cagttgtttt aacattggtt
    11521 ttattgggac aattactcga agcaaaagca cacagcagaa caagtggagc aatcaaagaa
    11581 ttattgaaat tagcaccaac tgaagctact ttagtaatta atggtgaaga aaaagtaatt
    11641 tccattcatg atattaataa aggtgatatt attcgagtaa aaccaggtga gaaaattccg
    11701 gttgatggta aaattaccga aggaaactct actattgacg aatcgatgat tactggagaa
    11761 ccaattcctg ttgataaaaa agtagaggat aatgtaagtt ctggaacaat aaacggaacc
    11821 aaatcatttt tgatggttgc cgaaaaagtg ggtgcagaaa cgttattgtc ccaaatcgtt
    11881 caaatggttt cagatgcaag tcgttcgcga gctccaattc agaatttggt agataaaatt
    11941 tctagatatt ttgtaccaat agttgtactg atttctgcta ttgccttttt aatttggtgg
    12001 caatttggac cagaacctaa attggtttac gcttttgtaa acgccattgc cgtattaatc
    12061 attgcttgcc cgtgtgcttt aggattagca acaccaatgt cagttatggt tggagttggt
    12121 aaaggtgcga aatcaggagt tctaattaaa aatgccgaag tccttgaaaa aatggataag
    12181 attgatgttt taattaccga taaaactggg acattgactg aaggaaaacc atctgttgaa
    12241 aaaattgtag taaaagatag agatgaaaat gaagttttag gaaaaatagc ttcactaaat
    12301 caaagtagcg aacatccatt ggcaacagcg gttgttaatt ttgctaaatc taaaattgta
    12361 tctttttcaa aagtagaaaa ctttgaatcg gttacaggaa aaggagtaaa aggttccatc
    12421 aacaatgagc taataattct aggaaataaa aaattattgg aacaagaaaa tagtcaagat
    12481 ttttcagcca ttgaagaaca agttatttca gaacaaaaac aaggaaaaac agtttcttat
    12541 attgctattg gtaaaaaagt gatgggttat attacgatta ctgatgctat taaaaaatca
    12601 agtttagaag ccattaatga attaaaacgt caaggagttc aagtcattat gctgactggt
    12661 gataatgaaa atacagctaa agccgttgca tccgaattaa atctaaccga ttttaaagcc
    12721 agttgtctgc cgcaagataa attagaattt attaaaaact tgcaagccaa aggaaaaata
    12781 gttgccatgg ctggtgatgg aattaacgat gctccagctt tagcacaatc agatattgga
    12841 attgctatgg gaacaggaac tgatgttgcc attgaaagtg caaaaattac tttggtaaaa
    12901 ggtgatttac aaggaattgt aaaagcgaag aacttaagtc atgcggtgat gaaaaacatc
    12961 aaacaaaatc tgttttttgc ttttatttat aactcattag gtgttccaat tgcagctgga
    13021 gttttatttc cggtttttgg aatattactt tctcctatga ttgcggcttt ggctatgagt
    13081 tttagttctg tttcagtaat agctaatgct ttacgattaa gaaccataaa aatttaaaaa
    13141 tgaaatattt attatatagt ttactatcca ttttattcat cggaaactta caagctcaaa
    13201 aagtagtgcg ttatgattta cacgttcggg attcaattgt caatttttct ggtaaagata
    13261 aaagagcaat tgctgtaaat ggacaaattc caatgccaac attgactttt acagaaggtg
    13321 atattgccga aattcatgtt tataatgagt tgaaagaaag tacatcactg cattggcatg
    13381 gattgtattt gccaaacaaa gaagatggcg ttccttattt aacccaaatg ccgattgaac
    13441 caggaacgga acacgtatat cgtttcccca ttattcaatc aggtacacat tggtatcaca
    13501 gtcatagtgg attgcaagaa caaatcggaa tgtacggttc gttgatttta aagaaaaaat
    13561 taaatgaccc aacgtttaga aaaggtattg atgatattcc acaagttcca attatattga
    13621 gtgaatggac agattacaaa cccgaaaatg tccacagaat gttacacaat gcttccgatt
    13681 ggtttgctat taaaaaagga actactcaaa gttatacaga agccataaga gaaggtcatt
    13741 ttaaaaccaa attaacaaac gaatggaaac gaatgttagc aatggatgta agtgatgtgt
    13801 attacgataa gtttttaatt aacggtcaaa atgaaagtca actttcacaa tttaaagctg
    13861 gagataaagt tcgattgcat atttcaaatg gtggtgcatc atcttatttt tggttgaaat
    13921 atgctggagg aaaaatgacc gttgttggta atgatggaaa tgatgtagaa cctgttgaag
    13981 tagatcgatt aataattggt gtctctgaaa cgtatgatgt aattgttaca attccagcag
    14041 aaaatacttc gtatgaattt ttagcaactc cagaagatag aaccaaatca acttcaattt
    14101 atattggcga aggaattaaa caattgacta ctccaatgcc aaaattgaag tattttgaag
    14161 gtatgaaaat gatgaacgat atgatgaata tggacggaac catggacgat atgggaatgg
    14221 acatgagttt tcaaaaaatg gatatgaata cggtgatgta tccagaaatt tcaggagaag
    14281 caatgaaact ggataagcaa gaagatatga aaatggatca ttcgaaacac gacatgtctg
    14341 taataaattc tgatattgtg actttaaatt attctatgtt aaaatcacca acaaaaacga
    14401 cgcttccaaa agatgcacca gttcgcgaat tgcgttttga attaacagga aatatgaacc
    14461 gctatgtttg gagtatggac aatgtcgtgc tttctgaaac cgataaaatt ttaattaaaa
    14521 aaggagaaaa cgttcgcatt actttataca ataattctat gatgcgacat ccaatgcact
    14581 tgcacggaca cgattttaga gttttgaatg gtcaaggaga atttgcacca ctgaaaaatg
    14641 tattggatat tatgccaatg gaaacagatg taattgaatt tgaagctaat gccgatggag
    14701 attggttttt tcattgtcat attttatacc atatgatggc aggaatgaac cgcgttttta
    14761 gttatgaaaa ttcagagccc aatccgtatt tacccaataa agaaaaagct tataaaaaac
    14821 tacaagctga aagtaatcaa tttcatttta tggcagaaaa tgattttgct accaatggaa
    14881 atgatggaca agcaatgtta caaaatacac gttggagctt tggaacagaa tggagattag
    14941 gatatcatga cgagcatggt tatgaaagtg aaacacatat tggaagatat ataggaagaa
    15001 atcaatggtt tatgccgttt ataggttttg atgcacgtta tagaaaattg gaaaatggag
    15061 aaatagaaga aaatattttt ggacaaaaaa gcaccaaaga caaacgcata caatttagtt
    15121 tagggtttaa ttacacctta ccaatgttag ttatttatca gactgaagtt tttcatgatg
    15181 gaaatatcag gatgcaatta atgcgagaag acattcctgt ttcaaaacgt ttacgaatgg
    15241 catttatggt caatacagac aaagagtata tggctggttt taaatatatt gttggaaaaa
    15301 acatgggatt tacaacgcat tatgatagcg atatgggtat tggttttgga gtcaacataa
    15361 attattaaaa gcaaaaaaat ccctaacaaa aaagtcaggg attttttatg tggtacctcc
    15421 aggaatcgaa ccagggacac atggattttc agtccattgc tctaccaact gagctaaggt
    15481 accgctctaa tgcgggtgca aatatagaat gatttttaat ttatacaaaa caattagtaa
    15541 aaaaatctgt tgtatttttg caattcaaat ttatcaccat gcttttagtc attgatgttg
    15601 gaaatacgag aataaaatca gctgtttttg agcaagataa gcaactcggg cattttgtat
    15661 tcttaaaaga agatttatta gaaaaagttg atttcattct aaaacagcat ccaaaagtag
    15721 gtacaattgt ggtcgcttct gttggaagtt tggagcaaag tgacttttta gcattcaaaa
    15781 gtaaagtaaa agttgttttt attactagag agtttgattt tccttttgtc aatttatacg
    15841 aatcgccaac aactttggga attgacagga tggttttggc tagtggagca gtacttcagt
    15901 ttccaaaaca aaatagattg ataattgatg caggaacctg tattacttac gattttgtag
    15961 atgaaacgga taattattta ggcggagcca tttctcctgg aattcgtctt cgctacgaat
    16021 cattacacaa ttatacagca aaacttcctt tgttagaaag agaatttcca aaaagcagta
    16081 ttggaaaatc aacctcagaa tctattcatt ctggcgtgat taacagtgtg atttttgaaa
    16141 tcgatggcta tattgattac tttaaatctg aaaacgctaa ttttatcata attttaaccg
    16201 gtggtgatgc tgattttttg gctatgcaat taaaaaatac catatttgcc aattcaaatt
    16261 ttctattaga aagtctgaac caaacatttc aatacaatca aaaatgatta aaaaaattat
    16321 agtaagcttt ggtttgctta tttcattggt ttctaatgct caagaaggaa cttcttctcc
    16381 ttattctttt tacggaattg gagacgtaaa atttaaaggt actgttgaaa cacgttctat
    16441 gggaggcgtt tctgtttttt cggatagtat tcatattaat ctgcaaaatc cagcgcaatt
    16501 agcaggatta aaattaacaa cttttgccgt tggtggttct acttcaaata caaaattaaa
    16561 taccgaaaca caaaacgaaa aagccagaag aactaattta gattatttag ccgtagggat
    16621 tccagcaggt aaatttggtt ttacatttgg tttaatgcct tattcttcgg ttggatataa
    16681 aatattgaat ttagcagatg aaaataattt atattctaca caatacaatg gaattggcgg
    16741 tattaataaa gtgtttttag gtttaggcta taaaatcaat aaaaacttaa gcgttggttt
    16801 agacgcacag tataattttg gaacgattga aaccaaaagt tttagatact taacaactat
    16861 tgaaagtgga actttagaag aaaatgtttc tagaatacaa ggaataaact tcactactgg
    16921 tttggcttat gagaaaaagt taaataataa gtacacttat tttgggtctt tagtttatgc
    16981 gcctcaagcc aatttgaaac ttaaaaacga aagaacaatt accgttagcg atcaagtaga
    17041 taatcaacca gtgcaaaatt tgaattttaa tttgccttca agattatcga ttggttctgg
    17101 atttggtgaa gttagaaaat ggttggttgg tgctgaggtt actttgcaat caagtagtaa
    17161 tttaaaaaat agatttgacg atataaatgg agtcgttttt gaaaattcta cacgctatag
    17221 tgttggagga tattacattc caaattatag ttcgtattct agttttttca aaagagtaac
    17281 ttatcgtgga ggtttgcgtt atgaaaatac aggtatggta attcaaaaca agtcaattga
    17341 agattttgct gtaaatttag gcttaggatt accactaagc ggaacttttt ctaacttgaa
    17401 catcggtttt gaagttggta aaagaggaac aaaatattac aatttaattg aagaaaatta
    17461 tattaaccta agtattggtt tatcattcag tgacagatgg tttgttaagc gaaaatacaa
    17521 ctaaaataca atgtcatgag agttttttta ttgttgaccg ttttattatt tggtctaaat
    17581 agtaatgccc aaaaaaaaat agattgctct ttacaaacca aagaatatca agaattttta
    17641 agcataaaaa attttgaaga agctttcgga ccatgggaat ttgtcgctaa aaattgtcca
    17701 actcaaagtg aaagtcttta tttagagggt gtaaaaatta ttcaatataa aattgataat
    17761 gctaaaaatg aggaggaaaa ggaaacatcg gttcgacaac tgatgaaact ctacgaccaa
    17821 tatcataaga atttccctaa ttctattgaa gattacgagg tgtataaagc tatggcttta
    17881 tttgataatt caattgattc aaaagatgag gttttccaat tgcttgataa cggaatcaat
    17941 tcttcgctta acaaagtaac ttcgggaaat gcaattttta actattttaa attgcttact
    18001 gaaaaaaata aaaatggaga caaatcggtc acaaatgaca aagtaattga aaaattcact
    18061 ttgttaaaca atcgtattgt tacattaaat gaaacaaatc ctgcagagaa agaaacatat
    18121 gttgcagcaa gtagagcaat taatgcaata gccgataatt ttttaacttg tgatgtttta
    18181 acggaatact acgaaaaaaa attggctcaa aatcaagaaa acgcagcttg gttagaagcg
    18241 tctttaaatg tgatgtatga aaaatgtgct gcaactccaa tttttttaac gttggcagag
    18301 aaatattatg ctttaaaagt ttcagcgcaa tcagcaaatt attttgcaac tgcattatta
    18361 aaaaatagaa aatttgatga ggcaaaaaaa tattttttag aatcagccaa tttagaaaaa
    18421 aacacttcca gtaaagcaac aaaatattac actttagcca ctggaattta tagcaacgac
    18481 aagccaaaag caaaagaatt tcttttaaaa tcaattgagt ttaacccaaa acttggaaag
    18541 gcatatttgt ttttagcaca actttatgcc aacagtgctt ccgattgcgc aaaaactgat
    18601 tttgaaaaaa aagcagttta ctatttagct actcaaacgg ttaaaaaagc ttctgttgct
    18661 gatgaagtgt taaaaaccaa tgttgccatg tttgtaaacg atttttcaaa agaatcgctt
    18721 tcagaaagtg acatttctaa tgctaaaatg aatggcaaaa ccatgacaat aggttgttgg
    18781 attaatgaaa aaatcacttt cccttctaaa taatgattaa taaaaaaagg acatattttt
    18841 tctttttact tctcactttt tttggaatgc tttcttgcga aagtaatttt aaagaagtgc
    18901 aaaaaatggg attgtctgag ttttcgccaa gtggagaagc agattctatc aatttaaaat
    18961 atacggattc aggaaagatt aaagctgttt tgattagtcc aaaaatgctg gattatgcaa
    19021 cagcagagtt cccttttact gagtttcctg ttggtattga cttaactctg tatgacgaaa
    19081 agggaaagaa aacgtatgta aagtctaatt ttgcatcgtc ttataaagga accgatttaa
    19141 ttgatttgca aggcaatgtt cgtataagta atgactcggg agatttgtta gaaacagaac
    19201 aattgtattt tgatcaaaaa aacgaatggt tttttactca aaaaaagttt aaatttactt
    19261 cgccaaaagg cgttactaat ggagaaggaa ttgattttag caaagacttc aaaaaagtaa
    19321 actctcaaaa aatatcgggt gaaatagata aatccgaata ataaaaaaac aaatgaatta
    19381 tttaaaattt acacaatatg cctatttaat agcaggatta gtatttgcct atgatgcttt
    19441 tacaaaatgg caaactgaag acccaaatta tattattagt gcaatttttg cagtaattgg
    19501 tgtttttatg tttttcttcc gacaaagatt tgctaaaaaa tttgaagata gaaacaaaaa
    19561 accataatca tggaaatcgg aatcatcatt ctgtgtttga tactttccgc ttttttttca
    19621 ggaatggaaa ttgcttttgt ttcctccaat aaaatatatc ttgaaattga aaaaaagcaa
    19681 gatgattttc tagggactat tttatcaaaa atcactaaga aaccttctaa atttatagca
    19741 acgatgcttg taggcaacaa tatttctatt gttatctatg gattctttat gggtgattta
    19801 atcttaaagt ggtttgaaaa ttttgggttt cattttacag aattatcaac tattttactt
    19861 caaacaataa tttcaactac ggttattttg atgacggcag agtttttgcc aaaagtcttt
    19921 tttcagattt atgccaatag tttcatgaaa ttttttgcct tgccagccta tattttctac
    19981 cagctgtttt actacatctc ttcgtttatg atttggattt cagattttat tctgaaaaaa
    20041 ttcttcaaaa ccgacggtga ccaaatcaat ttaactttta gtaaagtaga gttgggaaat
    20101 tatattacgg aacagatgaa tgctgtaaat gatgaagatg aagtggatac agaaattcaa
    20161 atgtttcaaa atgctttaga atttgcaggt gtaaaagcta gagaaattat gacgccaaga
    20221 accgaaatgg ttgctgtgga tttatatagt tcggtggcag aattgcgaaa actttttata
    20281 gaaacaggct attcaaaaat attagtatat caaaatacgt tggatgatat tgtaggctat
    20341 atccattctt ttgaattgtt caaaaaaccg agaagtgtga agtctataat gatttctgtc
    20401 gaatttgtac ctgaaactat tttcatcaaa gacgtaatga gtctgttgac taaaaaaaga
    20461 aaaagtgtag caattgtact tgacgaatat ggtggaactt cgggaattgt aacgatggaa
    20521 gatattgtag aagagctttt tggagaaatt gaagacgaac acgatcaaga tgaagcactt
    20581 gtagaaacac aaatcgacga atcaacctat atgttttcta ctcgattgga tgtggaatac
    20641 atcaatcaag aatacaaact taatattcca gaaagtgatt cttatgggac tttaggtgga
    20701 tttatagtcg attttgcttc cgaaattcct caaaaaggag agaaaattac cattggaaat
    20761 tatcaattta ttattgaaga agctaccaat aagaagatag aattggtcaa aatgacgtta
    20821 ttagattgat tttcgaaaaa aatcctaaaa atggcaaaaa ttataacact ctgactgtta
    20881 ttatttcata gataattgta ttttcgcaaa ctgaattaaa aattaacaga ataaaaaatg
    20941 gcagttttac aaaaaattag aaatcgttct ggcttgcttt tgcttgcaat tggttttgct
    21001 ttattagcat ttgttgtgca agatttattt acaaaaggct ttaaaagtat ttctacagac
    21061 gttggaagtg taaatggtaa agatatttca tttgatgaat ttaaaattaa agtagccaat
    21121 actgagaaaa accaacaaaa tggtcaatca atgacttcta tgcaagcttc aaatcaagtt
    21181 tggaatcaag aagttagtat tgctttatta actgctgagt ttgagaaatt aggaattaga
    21241 gttggagaaa aacacatagt tgaagtattc aaagcagatc caaatattgg tcaaaatcaa
    21301 atgtttttaa atgcagctgg aaaatttgac ttagcaaaat tcaaagaata ttttaaagca
    21361 aatcctgaag gaattcagta tttaaaagaa agagaaaaag atgctgattt gaatgcaaaa
    21421 tatcaaatct acaattcatt ggtaaaaggt ggtttatata ctacacaagc cgaaggaaaa
    21481 ttaaaatacg aagtagaaac aaacaaagtt acttttgatt ttgtaaatgt tcctttttct
    21541 tcaataaaag atagtgacgt taaaatttca gatgatgaaa taacggcata catgaaaact
    21601 aaagagaaaa aattcaaatc ggaagaagct agagaaattg agtatgtctt gattgaagaa
    21661 aaaccatcag cagaagacga agcagaaatt aaatctaaag tacaaagttt attgaatcag
    21721 agagttctct acaataacac aataggaaaa aacgacacta ttgcaggttt tgctaatgtc
    21781 aaaaacgatg ctgaattcgt aaatgctaat tcagatattc catttgattc aagttacgtt
    21841 gccaaacaag atttaccagc agaaaatgct gaaatgttat tcaatttgcc aactggacag
    21901 atttctgctc cttatatgta taacggttac tactgtattt caaaagcatt aggaagaaaa
    21961 tctggagcaa aatctaaagc aagtcatatc cttatcagct gggaaggaac acaagtgcct
    22021 aacaaaaaag aagtaagaac gaaagagcaa gcaaaagcta aggcagaaag cttattagca
    22081 caagcaaaag ctaatcctgg aaatttcttt atgttggcta tgatgaattc tgatgattct
    22141 tcggcgcaat caggtggtga tttgggttat ttttctccag gtcaaatggt agctccgttt
    22201 aatgattttg ttttcaataa cccaattgga aaaattggtt tggtagaaac acaatttggt
    22261 taccatgtta ttcaagtaac ggacaagcaa gatgcggtta aattagcaac gattgctcaa
    22321 aaaatccaac cgtctgaaat aacaaacaat aaaacctatg agcaagctac caaatttgaa
    22381 atggaggctg cttcaaataa agattttgat gctttggcaa aacaaatgaa attaacagtt
    22441 agtccatccg taagagttaa agctgttgac gaatcttttg gttcaatttc aaaccaaaga
    22501 caaattgtaa aatgggcttt caacagtgat acaagtgttg gcgatgttaa aagatttgaa
    22561 atcgtaaaca aaggacatgt aattgttaaa ttgaaaaaag tttatgacaa aggattaatg
    22621 gctgtggaag atgcaagacc acaagtagaa cctattttga aaaacaaaaa gaaagctgaa
    22681 atgattaaag caaaactgaa aggtgccact ttagaagcat tggcaactgc aaataaagta
    22741 actgtaatga atgctgttga tttaacagtt gaaaatccat cgataccagg tgctggtttt
    22801 gaaccaaaag tagttggaat agcgatgtct tcaaaagtag gaaagctttc tcaaccagtt
    22861 attggaaatt caggagtata tgtattggtt actaaagcag aaactaaagc acctgcaatt
    22921 caaaactatg atgagtatgt aaacaaattg aaacaacaag ttgcttctta ttcaggtaga
    22981 gttattcaag cattaaaaga aaatgctgac atcgatgata acagagctga tttctattaa
    23041 tagatttctt cgataaataa acaaacgact ttcatattgg aagtcgtttt ttattttata
    23101 aaatcatttc ttgataaggt aaaatagtgt caaatccttt agatttaaaa tacaccgttg
    23161 gattgtcatt tgaaatagcc ataacaaaat cgccacccca agcaccaaga cttttaatgg
    23221 tgccattaaa atcaggaaaa tataattgtt ttgctgtttc tttttctaga atagtgctta
    23281 aaagcatttc atgtttttct aattgatgaa taaaaacatc aatagagttt gtgctgacta
    23341 cggactccgt tattgaatta atttcaggaa ttaatttaga tatttcattg cgtttatttc
    23401 tataattagt aatagcatct cgactattct gttttttatt caaataaaca aaatagatct
    23461 tttctttaaa gcttggatta aaatctatag ttttactgaa tggtttgcct atttccaatt
    23521 gatatataat ggctgaattg ttttgagcac aagcaatatc ataaccactt ccgccaaaac
    23581 ttttttgcaa taattcaaaa gcatctatct gtaaccaatc agcaatattg ttaattaagg
    23641 tagaagatgt tcctaaaccc caatttttag gaaaggttaa cgcagttgtt acggtgtaac
    23701 cttttgagtg ttttataaat tcaggattaa ttttataagc ttcgtgaaga atttcaatta
    23761 atttattttt tatattttct gtaatatcaa aacgttcttt tttgatgatt gaagaaaaag
    23821 gaataacagt ttcaaaccaa atggagttat cataatcata acttttccaa ttcaaaactt
    23881 tgtctttgct ttcttcaact accaagcttt gaccaaattt tgttggtaaa gcaaatgcca
    23941 ttgcgccatc aagcacaaca tattcgccag taataagaag ctttccattg ctatagaagg
    24001 tttttgacat atgatttaga atttgaataa tctataaaga caaaaaatct gtgcnnnnnn
    24061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaatct gtgcgaatat gtgaaatctg
    24181 tgtttgaata aaagtgtttt tagtttctta atttttcaat atattcaaca accgcagcat
    24241 gagtgatagt gtgattttca aaatgcattt taactttatt tcgttcggta atattggctt
    24301 gaaattggtt taaaatgtta ttgatgtgca ttttcatatg gccatcttga attccagtag
    24361 tagtcaaaga acgcaaagca gcaaaatttt gtgccaaacc tgttgctgca acaatttgca
    24421 ttaactcttg tgcagaaggg ttttgtagaa tttctaaagc aattttaact aaaggatgca
    24481 aagaagttag tccaccaaca gttcctaatg ctaatggaat ctccatccaa aaactaaaaa
    24541 tatcattttc gatttttgca tgagataaac ttgaataagt tccttttctt gaagcataag
    24601 catgaattcc agcttcaacg gctctaaaat catttccagt agctaatact acagcgtcaa
    24661 tgccattcat tattcctttg ttgtgtgtaa ctgctctata aggttcaatt tctgcaattt
    24721 ttacagcttg aataaatttt tcagcaaatt ctcttggatt ttggatgtgt ttctcagcta
    24781 aatcttccac tttacatgaa acttctgcac gaaccacaca attaggtaca aaatttgaaa
    24841 gaatactcat tatgacagta atgtcttttt catcttctgt aaagttgtta taaaggagtg
    24901 cttcttcttt taatgtttta gcaaattgct ccaaacaaga attgatgaaa ttagcaccca
    24961 tactatcttt tgtttcaaaa gtagcatgca attgatagta gttttctaat tcagccgttt
    25021 tgttttttag tttaatatct aaaataccac caccgcgttt ttgcatgttt ttagtcaagc
    25081 tttcggtgtt tgaaaagaac tttggctttg tctcagaaaa gaatttttct aacttttcgg
    25141 acttaccatg gtatagaaaa tggacttgtc caattttttc tgttcctaaa atggttgttt
    25201 taaatccgcc acgcgccgcc caaaatttag ccgactttga agctgctgct acaactgaac
    25261 tttcttcgat tgccattgga acggaataat agttgccatt tattaaaaaa tttggggcaa
    25321 taccaaaagg caaataaaaa tttgaaattg tattttctat gaaatcgtca tgaagcattt
    25381 gaagactttc atcactattc caatattgtt ttaatattga aatactttct tttgggtttg
    25441 aaaaatatct atcggctatc caattgattt tttcttcttt tgataatttt gaaaagccag
    25501 taacttcttt attcataatt atttttaatt cttaataggc aaagataagc ctttgaagaa
    25561 attaatttta taaaaaatct tgaaattaaa aattaaacca aaacaggatt taacaaaaaa
    25621 tagtacctat atttataaaa aattcactaa aattggaagt tttttaaacg tagatgatta
    25681 tgaaggtaat tcgattacta gcaattttgt tgctaatagc aacaacaaca gtagcacaac
    25741 aaaaaatatc ggttgaagaa atttataaag gtgctttcag aacaaaagga atggatgagc
    25801 ttcaatccat gaaaaacacc aaccaataca caattcttaa ctcagataga gcaactcaat
    25861 cccaaaaaat agatttatac gattatgcta ctttaaagca agtttctact attatttcaa
    25921 ccaaagactt tcctattttg tctaatggaa ttgatagtta tatttttagt gctgatgaaa
    25981 agcaactttt aattgctaac aattctgtgc caatttatcg tcattctttt actgctgatt
    26041 tttttctgta tgatattgag accaaaaaac tttctaaatt atttgaacaa gtacaagtgc
    26101 caacattttc tccagatggt ttgaaaattt gttatgctaa agaaaataat ttgttcattt
    26161 atgaaattgc atcaaaaaaa acaactcaaa taacatctga cggtttaaaa aacgctgtaa
    26221 taaacggaat tacggattgg gtttatgaag aggaatttaa tgatccaaga gcaactggaa
    26281 ataatatttt tataagagct tttgattgga gtttggatag taaaaaaata gcttttatcc
    26341 gatttgatga aagtcaagtg ccagaattta cgatgaatat ctataaaaaa gacttgtatc
    26401 catcaaacga aacttttaaa tatccaaaag caggcgaaaa aaattctgtg gtttctttgc
    26461 atgtctatga tgtaacttcc aaaataaaaa aagatattaa cttaggcaat tattcagact
    26521 tctacattgc tagaatgaag tggtcaaatg atgccaatat tttatcagct caaattttaa
    26581 accgtcatca agataatttg gatttgttgt ttattgatgg aacaactgga agttcaaaaa
    26641 ttgttttgaa tgaaaaggac aacgcttatg ttgatgtaac cgataatctt acttttttga
    26701 aagacaatag ttttatttgg acatccgaaa aagacggtta taaccatatt tatttatacg
    26761 acaaaacagg aaaattaaaa aaccaaataa ctaaaggaaa ctgggaagtt acagcctatt
    26821 atggttctga tgaaaaaaat aaaaccattt attatcaatc ggttgaaaat ggttctacta
    26881 ttcgagatgt ttataaaatc tcattagatg gaaaaaacaa agtgaaatta tctcaaaaaa
    26941 caggaactaa tgcagctact tttagtccaa atttcgagta ttttataaat acgtattcca
    27001 gtacaatcat tccaacttta tatacattaa ataattctaa agatggaaaa gtgattcaga
    27061 ctatcgttga caatgatgtt ttagcaaaaa aattggaaac ttacaattta cctacaaaag
    27121 agttttttga acttactacc gaaaaaggca ataaattaaa tgcctggatt ataaagccaa
    27181 aagattttga tgcgtctaag aaatatccag tatttatgta ccaatattct ggtcctggtt
    27241 ctcagcaagt ggttaatgaa tggatgaacg ccaatgatta ttggtttatg atgttgacac
    27301 aacaaggtta tattgttgct tgtgtagatg gacgaggaac aggatttaaa ggagcagctt
    27361 ttaaaaaatg cactcaaaat caattaggaa aactggaagt tgaagatcaa attgatgcgg
    27421 caaaagtaat tggaaattat ccttatgtag ataaatcaag aattggtata tttggctggt
    27481 cgtatggcgg atttatgtcg agtaattgta ttttgaaagg tgctgatgta ttcaaaatgg
    27541 ctatcgctgt tgctccagtg accaactggc gtttttacga tagtatttat accgaaagat
    27601 atatgcaaac accacaagaa aactcaagtg gctatgatga taattcaccc attaaccatg
    27661 taaataaact aaaagggaat tatctattaa ttcatggaac tgccgatgat aacgttcatg
    27721 ttcagaattc aatgcaaatg attgaagcgt tagtccaagc caataaacaa tttgattggg
    27781 caatttatcc tgataaaaac cacggaattt atggaggata tactagaatt caactgttta
    27841 acaaaatgac aaattttata aaagaaaaac tataataacc aaaaacaaaa caataattat
    27901 gagtgaaact acagtaaaag cggcacatcc aaaagggctt tgggtgcttt ttggaacaga
    27961 aatgtgggag cgtttcaatt tctatggaat gcgtgccatt ctaaccttgt tcatggtaaa
    28021 ttcattattg atgagagaag aagacgcttc tatcatttat ggtggatttc tgggactttg
    28081 ttatttaaca ccaatgttag gtggatttat ttctgaccgt ttttttggta atagaaattg
    28141 tattatacta ggaggaacta tgatggctat tggtcaatta atgttgtttt ttagtgcaag
    28201 tatatttggt tctaacctag gattagcaaa tacattaatg tggaccgcat taggaattat
    28261 tgtttttgga aatggatttt tcaaaccaaa tatttcctca atggttggaa gcttatatcc
    28321 aaaacaagaa aaatctaaat tagatactgc tttcacaatt ttctatatgg gaattaacat
    28381 tggagctttt cttggtcaat ttatttgtcc atttgttggt gatgtaaata acgaagtgtt
    28441 acaagccgat ggcacaacac ttatgattag agatgttcat gcattcaaat ggggcttttt
    28501 agcagcaggt gttgccatga tgataggtac agcagtgttt ttctttttaa aaaataaata
    28561 cgtggttacg ccagaaggaa gaccaattgg cggattacct tcaggaaatg ataataacga
    28621 ctatgaagaa ggagaatctc aaaaagctca ttttacaatg ggttcaattt taacagcagt
    28681 tgtcattttt gccgcagttt tctttgtatt caattatttt actgagggtg ataacatagt
    28741 aaaaacgatt atttacccag ttatatacgc cagtggtatt tcgcttgcgg cactaatttt
    28801 gttagataaa actttgacaa aagtagaaac tcaaaggatt tgggtaattt atatcatgtc
    28861 gttttttatt attttcttct gggcagcttt tgaacaagca ggctcttcat taacgtttat
    28921 tgccgacaat caaacagaca gacatttctt tggttggaac atgccaccat caatggttca
    28981 aatttttaat ggactgtttg tagtgatgtt tgcttttcca ttcagtattt tatgggataa
    29041 attgagagca aaaaaccgag aacctctttc tacagtaaaa caatctattg gattattatt
    29101 aatcgctgta agttatttta tcatagcaaa caatgtaaaa gatcttggaa acacaggatt
    29161 attggctatc aaatggttga ttttattata cttaattcaa acttttgcag agttatgtct
    29221 ttctccaatt ggtttgtcat tggttggaaa actttctcca aaaagatttg cctctttagc
    29281 ttatggtgta ttctttattt caaatgctgc gggttatgct ttatcaggaa ctttagggtc
    29341 aattcttcca gcaactggag ataaatttaa aaaagcatct gaacttggaa ttgacttgca
    29401 aggtgtatta gataaatcaa ttacaccaac agcggaacaa ttagagctat tagcaaaaaa
    29461 tcaaattagt gatcattatc caacatttgc tagtttccca attgaaaatc tatacgattt
    29521 ctttatggta ttcgtagtgc tttgtggtat tgcagcagca attttattta tgcttacacc
    29581 tgtaatcaaa aaaatgatgc acgggattag ataatccaac tattaaaaaa tatttttata
    29641 tctttcaaac ctccaattgc attggaggtt ttttttaatt aaaataaatt actatgtgga
    29701 aatcgcatcc taaagcatta ccttttttgt ttttgtctga aatgtgggaa cgttttggtt
    29761 attatttaat gattggaatt tttacgcttt atttaaaaga tgtagaagct ggatttgcca
    29821 tgactgagaa agaagcttcc gacttatatg gaacttttat cgcattggtt tttctaacac
    29881 cttttattgg cgggttgatt gctgacaggt attttgggta taaaaaatca attattattg
    29941 gtggattaat gatgggagcg ggttatatga tgatgggaat tcatgacaaa accatgcttt
    30001 atattgccat gactttagtg attgttggta atgggttttt caagcccaat atatctacct
    30061 tacttggaaa tttttatgat gatgaagaat acaaggagaa aaaagatgaa ggctacaata
    30121 ttttctatat gggaattaat gtaggtgcat ttatctgtaa tttctttggt gcagctttac
    30181 aaatcctttt aggctggcaa tatgctttta tggctgctgg agtcgggatg tttattggtg
    30241 taatagtatt tattttaggt actaaacatt atggtgataa aactgagaaa aaaggggtgc
    30301 aacctggcga catgcctttt tataaaattg tgctttacat tttggtgcct tctattattt
    30361 ttggaataat tgggtggtta ttaaaaggag tgcaatcaga attaaatccc gatagttata
    30421 tttttggttc tgatagtacg gatgcgttta tttttgcttg tattcctgtt gtttattttt
    30481 atgcaagttt atattttaaa gcaaaaactg aagacaaaag accaattggt gccttattag
    30541 ctattttttc tgtagtgatt ttgttttggg cagtttttaa attaaacggt tcggcactaa
    30601 atacttgggc agaccgttat actgatagag aaattacagg aacaactgaa aaagtattta
    30661 atggattaaa gttttcacaa gaagtagcct atcaaaaaga tagtgtttcc ctatacgatg
    30721 ctagttttag actccaaaaa gtaaatggtg aagtgcaaaa agaatacaat tatccagttt
    30781 atttcagaaa tgttaaacct gaaaaactac cagccgaagg ctcaaaagtt tcactatggg
    30841 ctacaaattt aagtcaatcg ataaatcctg gatgggttat tattttaact ccgttaatag
    30901 tagcattttt tactttttta agaagtagaa aaaaagaacc ttcaacaccc accaagattg
    30961 cgtttggtct tttaatttca gctttatctg ttttggtgat ggttgctgct gtagaaattg
    31021 gtaataatgg ttctgagaaa gtttctgttt ggtggttagt tgctaattat ggaattataa
    31081 cgatcggtga actttttctc agtcctatgg gattgtcaat tgtttctaaa ttatcaccaa
    31141 aaaacattac atctttaatg atgggtggct ggtttttggc aacttctatt ggaaataaat
    31201 taagcggtgt tttagcaagt atgtgggatc aatatgataa taaagccaat ttcttttgga
    31261 taaattttgg gcttttgttg tttgctacag tattaatgtt tgcattagtt aaaaacttga
    31321 acaaagtaat gaaagaaaaa gggattaatt aaaattggac taacttgtaa agtatcttac
    31381 atataggttg gcattctttt tgttatattt gaaaaaaatt aaatgattat gaagaaaata
    31441 atattcgcat ttgtatttgt tttaggaact ataaccatgc atggacaaga attaaaatgg
    31501 gaaacaaatt tagatgttgc catgaagatt tcaaagaaaa ctaaaaaacc aatgttacta
    31561 tttttcacag gtagcgattg gtgtggttgg tgtatccgtt tgcaaaaaga agttttacaa
    31621 acaccagaat ttgcaaaatg ggcaaaaaaa aatgtagtat tggtagagtt agattttcct
    31681 agaaaaacac ctcaggatgc agcaattcaa aaacaaaatc aagaattgca acaagctttt
    31741 caagttagag gttatccgac agtttggttt gctaaggcat ctaaaataga tggaaaagta
    31801 aatttcgaac aattaggaag tacaggctat gtagctggcg gaccaagtgc ttggttagaa
    31861 ggtgcagatc aaattcttaa aaataaaaaa tcgtaacgat ttacctctaa aatttataaa
    31921 atcctttttc atgtgtattg tggaaaggga ttttttgttt tctacacgtt tcttcccaac
    31981 gtttgctgta ggttttaaaa tttgaaccat ctgcaataat aacctttggt tttagtgtca
    32041 ataaaagccg ttctaaattt agttttgggg attgtgtgat aattaaaata tcaggattaa
    32101 tggtaggatt ataaattcca gtactatcaa taacgacaat ttttttacta ttgataaaaa
    32161 gcaaatcagg aatattattt ttattcttta aatctgaaaa atttccaact aaatacgatt
    32221 gaaaagtaac attttcatga atgttcttcc gaatactatc attcgcaaat aaggatacat
    32281 tattaccaat tctctccgaa atgatactgc ctttcttttg attaaaaacg attccttctg
    32341 cacttttgtt aacttctttt ttttgataaa taaaaacgac ttgtaaaagt atcacacaca
    32401 ttaatgctaa cgaaattctt ttaaaatttg gcttttttat ccaaaatatt agagtaacta
    32461 tcatcaaata gaacaaccat agcatttctt ttgtaaaagg aatattttgg aaagtaaagc
    32521 tgtcaaacga tgcaatggtg tgaatgaagt tgttcatgat tttcaacagc aaatcaagga
    32581 tttgcactaa aaaatgagga acagttccga taacggatag tactaaaact aaaattccaa
    32641 ttgccataat tactgacaat aacggtagaa caatcatatt tgttaaaaaa aacaatcctg
    32701 gaaattgatg aaaatagtaa atactcaatg gcattacgcc gatttgagct gcagtagaaa
    32761 cggttattat atcccaaaaa taatttagaa tcttattttt tggtttccaa atagatttta
    32821 aaattggctg caataaaaga ataaaaaaca ctgctaaata acttaattga aagccaatat
    32881 cataaataaa tgacggactt atcaataaaa ttaaaagcat tgaaactagc aaggtatgat
    32941 aaatattgac agttcttttg atgttcattc caaccgttaa aaaggaaaac attgtagcag
    33001 atcgaactat tgaaggtgat agcccagcta aaagtgcaaa tgcccatagt gtcaatataa
    33061 tgagtataac tttaatccag tttgctcttt tggtattggg cattggtttc agtaaaaaca
    33121 ataagaaata cataataatt ccaacatgta atcctgaaac ggctagaatg tgaactgcgc
    33181 cagctgcttg atattctttc ataatcacag gtgacatatc atgtttttgt cccaataata
    33241 aagcaacaac aacagttaat gaattttcag agagtgatgt tttcttcaaa tttgtgatta
    33301 atttttctcg atagtttgaa aaatagtaac ggagcgaatt ttgacttcca ataattttta
    33361 catcattggt tttgctatat acttgaccat aaatttcttt gtgttccaag taatttccat
    33421 aatcaaactg attgggattg aaaggctttt tgtttttgta gtaaacgcct tttatgaaaa
    33481 gaatacttcc aattggcaac gaaatgtatt tttctttttt tgtaatgtta aagattattt
    33541 tgccattaac tttctttttt tgtatcgatt taaattcagc aacatatcga tagttttttt
    33601 gcgtattctt gagtttttca gtcaataaaa ttgttgcatt aaaaccattt tcaacttcat
    33661 ttagatactt agaaaaatga ttggtggaat aattttcttt atgaatacta gctgaagaca
    33721 atccaataaa aaaagaattt aatagtactg ctattccaaa taaagatgaa taaaatgcta
    33781 ttttggattt aaaaatataa caaagcagta ctaggcaaaa tccacttaca gttaatataa
    33841 aagcaattgg tggtgttgtt tttaaagaag gaaataataa tattccaaaa acaaaccaaa
    33901 gtgtaatttt agctaaagga aattgtaaga ttttcacgta actaagttac gaaaaaaaag
    33961 aatttacaaa ataaatttat aagattttgt taatttgaac aaaagtgttt tggtaatatg
    34021 tttctgatag tgaagaaaca ataacacctg cacttgttga agcatgaaca aataagattt
    34081 catctccatt tacttctgtg atcatgccaa catgtgtaat acgatttccg ccagctgtgg
    34141 caaagaatat taaatcacct ttttcggcat tatcaatgct cattttttca ccaatatctg
    34201 ccatacttga agaaactcgc ggtatatcaa tgtcaaattt tttgaaagtt gaatagagca
    34261 aaccggaaca atcaaaacca tcttttgaag tgccaccata tgaataatct acacctacat
    34321 tgtctatcac attattaata agttgctcag caacatagtc agtttcctcg ttgtcgatga
    34381 taatgtcttt agcatttttg gtattcaaaa ttgctttaat tttttttgat tttttttctt
    34441 tatctctaat ttcttttttt tgatttttag ataaaggttc gttgtaggca atggctttat
    34501 caacacttgg cgcagtgtaa attccttttt tttcagcttc ttttttagta gtaatgattt
    34561 tgtttgattg tgcaaaacca aatgttgaaa taaaaaaaaa taaaaagaaa aatattttga
    34621 aggtgtattt caagatcgtt tatttaagat tttcaaatat aaaaaaatat tttttaaaac
    34681 acattaatta ttgtaaatca ttaataatca ggtctgctgt ttttttactt gcaccaattc
    34741 cgcctaactt tttttctaaa atttcataat tacgaattat ttcctgtctg ttttttggtt
    34801 caaaaagttt tgataattct accttgatcc tttttttatt aaaattttct tggattaatt
    34861 cggttaccac ttctttatcc attattaaat ttactaatga aatgtatttt aatgtaatga
    34921 tgcgttttgc aatttgataa gaaacccaac ttcctttgta acaaacaact tctggaactt
    34981 taaataaagc agtttccagt gtcgctgttc cagaagttac taacgctgct tgggatatac
    35041 tcaataaatc ataggttttg ttagaaatga attttacatt tttagtgttt aaaaattgct
    35101 tataaaattc aaattcttga cttggtgcac cagcaattac aaactgataa tcaataaaat
    35161 catcagtaac actaagcata acttcgagca tttttgaaat ttcttgtttt ctactgcctg
    35221 gtagtaaggc aattaccggt ttgtcatcaa gatggttatc ttttctaaat tgagtttcgt
    35281 ttgttttttg gcgattgtga atagcgtcaa ttagcggatg accaacaaat tctacaggat
    35341 aaccatgttt tatttcaaaa aaatcttttt caaaaggcaa aatgacaaat aatttatcaa
    35401 aatctctttt gacagctttt atcctattct ctttccaagc ccaaatttgt ggtgcaatat
    35461 aatagtgtgt ttttatacct ttttgttttg cccattttgc aattcgcata ttaaaacctg
    35521 gataatcgat gaaaataatc acatcaggtt tgaattgttc aatgtctttt ttacaaaatt
    35581 tgatgttgtt taaaatggtt tttaagttca ttaccacttc tgcaaaaccc atgaacgcta
    35641 atgccttgta gtgttttact aaagtgccgc caacttcttg catcaaattt ccaccccaaa
    35701 atcgaatttc tgctgtagca tctttttcaa ataaagcttt cattaggttc gaaccatgca
    35761 aatctccaga agcttcacct gctataatat aatatttcat tgttttaata atttaataaa
    35821 acacagtcaa ttaattgtcc gtttttaaaa gtaataatcc aaatttttcc gtattcgttt
    35881 tcgtttaaag gattgaattt agtttcgtta attaaactat taaccaaatc gggacaagtg
    35941 tttgagtgac caacaatcat tattgtttta cctttttgat ttaaaagtaa attttggcta
    36001 aaatttttat tatttggact atagtttatt atttccaaat ttctagagtt ggcaattggt
    36061 tgtaatgttt cagttgttct tgaaaatggt gtagaatata aagcgtcaag cttaaggtct
    36121 gcaaacagca tatctaattt tttagcacgt aatttcccaa tatcagatag tgaagggttc
    36181 ttgctaccat catcaatttt ttctgcatga cgaacaacaa ttatttttgt gatttcagta
    36241 ttactatttt tttcttgaga aatggtaatt gaagaagtca gaataagcag taaaaatgaa
    36301 aaggtttttt tcattagtaa aaaattaaat tttataaatc ccaagtgttt aacaattgaa
    36361 tagcatgatg cgctgtcatg tcaaactgaa caggaacaac tgaaacatat ccattagcta
    36421 atgcgtattc atcagtatct tgacctttat catcattcac aaattcaccc gttagccagt
    36481 agtaatcttt tccataaggt gattttctct tgtcaaattt ttctatccaa ctcgcttttg
    36541 cttgacgaca aattttcact ccttttattt catttaaatt taatttagga aaattcacgt
    36601 ttaacacggt gccattaggc attttgttgt ttaaaacttc taatgcaatt tttttaataa
    36661 acggtttagt agcttcaaaa ttagcattcc aattataatc ggctaacgaa aaaccaatag
    36721 caggaattcc ttcaatacca gcttctacag cagcactcat agtgccagaa taaattacgt
    36781 ttatagaaga gtttgagcca tgatttattc cagaaacgca taaatcaggt ttttgtttta
    36841 aaacctcatg taccgctagt tttacacaat cgacaggcgt tcccgagcaa ttgtattctg
    36901 taatttcagc accatctttt gatattttat caagatataa tgtgttattt acagtaatag
    36961 catgtcccat tccgctttga ggactatctg gtgcaactac tacaacagtg ccaatttccg
    37021 acatcacttc aattaatgtt cgtattcctg gagcagtaat tccatcatcg ttggtaacta
    37081 aaattagagg cttgttaatc ataataaaaa attcaattaa ggcaaaatta acctttttta
    37141 gggttttatt aagtaacatt ttgattaact ttggactaat tcaacgtatg ctaaaggtta
    37201 attgttataa aatatcgtta acataaaaat ttggcatagt tttttgtata tattagtcga
    37261 ataattaatg aaacctatat tcctgtttat gaaaagaaat tataaagttc tcttggtagt
    37321 acttgctttg tcagttgcca tgtggagttt catccccaaa gagaatacac ccgatcccga
    37381 aaaagataaa ttattaattg aacttttaac ttttgttatt gaaaaaggac attacaatcc
    37441 tgctgcaatt gacgacacct tttctaaagg agtttacaaa gactatttag aagcattaga
    37501 tccctcaaaa cgattctttt tgcaatctga tattgatgaa ttttcaaaat atgaaactca
    37561 aatcgatgat caaattaaca ataaagaatt aacctttttc gatttgactt atactcgttt
    37621 gatgaaacga atgaatgata gtaaagctta ttatgaagaa gcgttatctt ctccaataga
    37681 ttataaaaaa gatgaaacta ttagcacgga ttatgaaaaa ttgccttatg ctaaaaatga
    37741 aaaagaatta aaagaccgtt ggagaaaaca agtcaagctt tctactttgt cttctttggt
    37801 tgaaaaagaa aaagttcaag aagaaaaatt aaaatcgaaa gacaataaag atgctccaaa
    37861 aacttttgct gaattagaga aagagactag agcatctact ttaaaatcac ttaatgataa
    37921 ttttggattc ataaaagatt tgaatcgaga tgattggttt tcaatatata tcaatgctat
    37981 ttcttctcga tttgatccac atacttctta ttttgcacct aatgagaaag agcgttttga
    38041 tgtaagtatg agtggaaaat tagaaggaat tggtgcaaga cttcaaaaag aaaatgattt
    38101 cacaaaaatc acagaattaa tttctggcgg accagcatgg agagggaaac agcttgaagc
    38161 tggggattta atattgaaag tagcacaagg aaacggcgaa gctatagatg ttgttggaat
    38221 gcgtttggac gatgttgtga aaaaaattaa aggaccaaaa ggcactgaag ttaaattgac
    38281 aataaaaaag ccagatggaa ccataaaagt aatttcaatt ataagagatg aagttgaaat
    38341 tgaagaaaca tatgtaaaat caagtgttgt agaaattgat ggaaataaat atggaataat
    38401 ctatttacca aaattctata ttgattttga gaacaaagac agtcgtgatg ctggaaaaga
    38461 cgttgctatt gaagtagaac gtttgaaaaa agaaggtgtt caagggattg taatggatgt
    38521 tagggataat ggtggtggtt cattaaaaac ggttgttgat attgcaggtt tgtttattga
    38581 acaaggaccg attgttcaga taaaatctgc agcaggtaaa aaagaagtat tgtacgacag
    38641 agatgcaaaa ttacaatggg atggaccatt ggtagtaatg attaatgaat tttctgcatc
    38701 ggcatcagaa atcttagctg ctgcaattca agattataaa agaggtgtaa ttgtaggaag
    38761 caagcaatcg tatggaaaag gtaccgttca gaatgtaatc gatttgaatc agtttgttcg
    38821 cggaagtact tatggagatt tgggagcatt gaaaacaaca actcaaaaat tttatagaat
    38881 caatggaggt tcgactcaac gagaaggcgt taaaagtgat attgtgatgc ctgataaata
    38941 tgaatattta aaaatgggtg aaaaagatgt tgataatgca atgccttggg ataaaataga
    39001 tcctgcggaa tacaaagtat ggactaagca acacaatttt gatttggcga tagctgaaag
    39061 taaagccaga ttagcaaata atccacaaat gcaattgatt gaagataacg caaaatggat
    39121 agaccaacgc agtaaagaaa atatttatag ccttcaaatg gacaaattta aagcggaaca
    39181 aaaagctttg gaagagaaaa ataaaaaata taaaccgatt acagattata aaaatgattt
    39241 gaaatttatg tcgttgcctt atgaagacga aatggttaaa aacgacactg ttttaaaaga
    39301 aaaacgagac agatggcacg aaagtttatc taaagatgta tatgttgatg aagcattaca
    39361 tattttaaat gatatgcaaa atggttcaga cagaaaaggg ttgacagata aagttaaaac
    39421 cgaaaaattg gttaagtctt aattgaaaaa aatattcaaa caaaaaaagc ctcaagatta
    39481 ttttttgagg cttttttatt caagatactc taatttagtt tcttagttta ttcaattttt
    39541 tttcataatc agcaatgtct tcttcatatt cttttatctt aagttgctgt ttggtaatat
    39601 tcaaactttc tttctcaatt tctacaggag ataattttcc ttttgatttg tttttggcga
    39661 atttagtttt catcttttct aaatcgtcaa tagcatcttg tcgtttgtct ttatacttaa
    39721 ttaatttttt ttcagcagaa gcaatttttt tttgttcttt ctgaaacttt ttttgttctt
    39781 tttctaattt tttctgatct ctttcaagct gtttctgggc tttttctgct tgttcttttt
    39841 gagctattgc aagtttttga ttttcaattt ttttttcaag caattctttt tcagttgctt
    39901 ttggagcttc aattgtagct gtttttactt ttgcagaatc aataaccttt tcagaattag
    39961 tttcttgtgc ataattgttt tgatagcaaa ctgctaaaaa cgctacaaat aataatttta
    40021 atttcattgt tttagttttt aattatttta ctataaattt agcaataatt gttccaaatt
    40081 ctaaaataat atatgaaaaa ttacattgta ataaatgttt tcatcttttt gatttcttat
    40141 gcaatcattt cttgcaaatt tctcgaaaaa gatgatacga ctgaaaacaa aaaacaattt
    40201 tcatcaatat ctgctattga tttactacca acgtcaacca caaaacaaat aattgtacat
    40261 gatttttata cattgtccta taatgaaaaa tttgaacaag ctgaatgggt agcctattct
    40321 ttgaaaaaag agaattttaa acaaaataat tttaaaagac cctattttgt tgaagataaa
    40381 aaagtaacaa cacattctgc cgattggaga aattataaaa aatcgggata tgataaagga
    40441 catctttgtc cagctggaga tatgactttt tctaaaaaag catatgaaga tactttttta
    40501 acctcaaata tttctcctca aaagcatgat tttaattcag gaatttggaa tcgattggaa
    40561 caaaaaacaa ggtattgggc tgagaagtat ggaaccgttt ttgtgatttc gggtggtgtt
    40621 ttaacatctg gattaaaaac tattggtttt gaaaaagtag ctgttccgaa ttatttttat
    40681 aaaattattg cttcaaaaag caatggtcaa attaaggtaa tagcttttct tgtgccaaat
    40741 gaagattcaa aaaaaccact ctatactttt gtaacgtcta ttgatgcaat tgagaaatta
    40801 acgaacatcg actttttttc aaaatgggaa gattcagtcg aaaataaact tgaaagtaaa
    40861 agtgactata aagattggag ttttaattga tttgaaactt gtattcttcc taatcttaaa
    40921 gatttactaa atacaatgat tggaatttgg aatttatttg tgggaatttt ttaaaaacta
    40981 ccattcgttt actttgtttg catccatttt taggaaaata aataataaaa tagtaaagcc
    41041 ccaaagacca gaaccaccat aagaaaagaa aggcaacgga acaccaatcg ttgggaatat
    41101 tccaattacc atcgaaatat taacaaaaaa gtgtatgaat agtatgcctg ccacacaata
    41161 accatagact cgactaaact ttgtcttttg cctttcagcc aaataaagta ttcttccaaa
    41221 taaaattaaa aataaggcaa caattaatac agaacccaaa aaaccccatt cttctccaac
    41281 tgttgtgaaa atataatcgg tgtgttgttc aggtacaaag ccgccttttg tttgcgttcc
    41341 ttctaagtat ccctttccaa acaatccacc agaaccaata gcaatttcag attgattggt
    41401 gttatatcca acacctttca tatctacttc tttacccaaa agaatattaa atcggtcacg
    41461 atgatgttgc ttgaatacat tgtcaaaaac ataatcaact gaaaaaacat atcccgaaat
    41521 caatactaaa ataatagaac tcaaaaccca atttctatca atcatcctgc ttctaaaatg
    41581 aataaaaaga ataacgacta atgcaattat tacaataacg tagggttcta aaagcagcgc
    41641 taaaacaaag agtatgatgg caataaatcc tgtccaaaga taccatgaag gaaatccttc
    41701 tcgatataaa accaagaaaa aaacagaata aatcaaggcg cttcctgggt cgggttgcgg
    41761 taaaattaat aggattggta aaaataaaat aatcatggct tgcacctgtc tgttcatgtc
    41821 ttttaaattg acttgaacat cacttaaata ttttgccaag gctaatgctg tagctgtttt
    41881 agcaaattct gatggctgta atgtgaacgt tccaatggca taccaacatc gttgtcctgc
    41941 aatcgttttt ccaaagacaa acagaccagc aagtgataaa agagacactg caaaaatgat
    42001 aatagaatac ttttcaaaga atttcgcatc aatagccatg atgctaaaaa tcaatggaat
    42061 ggtaaaaaga atgaataaaa attgttttcc ataaatttga gttacatcaa aaattgaact
    42121 ttccccttca atgcttgata acgaagaaga gtaaatatta agccaaccta aaatcactaa
    42181 caggatatag attccgatgc ttaaccagtc taaattattt gtaacacttt ggtttttcat
    42241 actaattaga aattaatttt tagtagtatc ttttacttta atttcttctt cttttttcat
    42301 ttctgtattc aacgatttta aaatagtagt attaattaaa gtatcaacaa ttggtttagc
    42361 aatgtattta ttgtattccg cttgcaaact tccttctaac attctttttt ctaaatcttt
    42421 acgagtgatt tttttattga tgtatttttc aatcattaat gttgtaattg gaccagccca
    42481 tcttgcaccc caatatccat tttcaacaaa aacagcaatg gctattttag gattgtcttt
    42541 tggcgcaaat gcaacaaaaa tagagtggtc ttgaagctta actctttttc cgtttatttt
    42601 ggcaaagttt tcggcggtac ctgtttttcc acaaatttgt atgccttcaa cacctaagcc
    42661 tcttgccgtt cctaagttat aaacatcgta taaaccttca attactggtt caaaatgttt
    42721 tttgtcaacc gttgtttgat gtttggttct aaatttttta tcaatcattt cgcctttgat
    42781 tttcttaatg atatgaggcg tataataata acctctattg gcaacagttg ctatcatatt
    42841 ggctaattgc attggcgtca tcaagacttc accttgaccg atggcattgg aaacaatagt
    42901 tttactgtcc caattccatc ctggatacat ttttttatac gtttttgagg ttggaatttt
    42961 tcctttcctt ccagttggta agtcgtagcc cataaattgt cctagtccaa aacttttcaa
    43021 gtgattgctc caagtatcta caccatccga aggctttaca tatttgttta tggttttcat
    43081 ataaacattg gcaaaatagg tgttgcaaga gttataaaag ccattgtgta atttagaaac
    43141 gccagcatcg tgacagcgca taaaacgacc acgtgcataa ctgaaaccgt gatgacaaac
    43201 aaatgatgtt tcggtatcta taacaccttc ttgaagtcca actagccctg ttagaatttt
    43261 aaatggagaa ccaggagtat attccgctag caaacctcta tcgtataatg gttttgcaat
    43321 tgagtcgtga tacaacatgg tgtagttttt agagcgttgt cttccaacca atatggctgg
    43381 atcgtaggag ggtgatgtta ccaacgctaa tatttctcca gtttttggct ctatagctac
    43441 aattccacca cgtttgttaa tcattaattg ttcgccatat ttttgtagtt cagcatcaat
    43501 agacaatgta atgtcttttc cttgaacagc aatagtatca aattttccat ctttatagga
    43561 acctatatcg cgattgaatt tgtctttttg aagatatttt actcctttga cacctcttaa
    43621 aatttcttca tagctttctt caacaccttg ttttcctatc aaatcaccac tattataata
    43681 tttgtttttg ctgatgatgt tttcatttac ttgtgtaata aatccaaaaa cattggcacc
    43741 acaatcaact tgataatcac gtagcgatct tttttgaata taaaagccat taaatttccg
    43801 ttgcatttct tgaaaagcgg catattccat tttgtttaat tgtggcaaaa aaaccgatgg
    43861 caatcttgga ctatacactt tagctttggc tatctttttg tcaaaatcga cttttgagat
    43921 gtttaataat ttgcaaaatt cgagagtgtc aatattttta acatcttttg ggataaccat
    43981 gatgtcatac gaaggttgat tggcaaccat aagtttaccg taacgatcat aaatataacc
    44041 tcgttcgggg taatcatatt ttattttaat tgcattattg tctgatttca actttaaact
    44101 ttcatctata acttgaaggt aaaaaagcct cgacacaatt aaaattgttg caatgattat
    44161 aattgttgga aacaatactt ttctcatcgt ttgcttggct taataatata aatgattaat
    44221 atacaggtga taatagtaaa tatagtgcta aaaatagtcc ttaagaaaat atcccaaatg
    44281 aaactaattt taaaaatttc taaaataaat aacgtaaaat gatgaatgac aacggctatt
    44341 aatataaacg aaaagcgttc gggtgttaat acatcgttta gtttaacggt ttgatattca
    44401 taacttagtc caaatgaaaa tttgaaaatt gctggtcgaa aataggctag aaccaaacaa
    44461 gccgttgcat gaacgccgcc agaattccaa aacatatcca taatcaaacc tagtagaaaa
    44521 ctagctacta aaagccctgt tttattccca tttactggaa aaagtaagat gaacaaaata
    44581 taaggataag gattgatata tccaaacaaa tcaatgcgat tgaacaatag tacttgggct
    44641 aaaagcaaga aaataaatcg ggcgatattt actaataaag tactattcat ttttaatggt
    44701 ttcgctttca agtttcatca cttcgtctct atcttttgtt tttaaaatat atacataacc
    44761 taaattcgtc atgtcattaa acaattttat atccaaagta taataattgg ttacgttgtc
    44821 tgtgtagatt ttatcaataa ccccaatccc aatattttcc ggaaaaataa tagactgtcc
    44881 acctgtaaca atagtatctc cttttcgaac agaagctagt cttggcacat caattaattg
    44941 tgcataaccg gtatttttgc cgttccaaac taatgaacca aaatgatttg atttcttaat
    45001 tttagcatta atttttgact gaacatttaa tatgcttatc actgtagcat agtttttgga
    45061 agttttgtca acaataccaa taattccagc actattaatg actcccatat cggatttaac
    45121 accgtcttta gaaccactgt ttaaggttaa gaaattttca tgtgtgctat aggaattgtg
    45181 aattactttg gaaactataa tttcagcttt atcaattcct ttaacattta catttggtgt
    45241 aagtgtagta tccttttggt tgaataaaat agattttaaa cgggcatttt ctgcagacaa
    45301 ttcttgattt tcttttcgta atccaaaata ttcattcacg ttatttattt gttgatatac
    45361 accaccggtt agaaaattag cggaactaat gactctgctt ttgtgataag aatgtgattg
    45421 aatcgttaat gttaacgaaa taactaatag cagcaagaac agtaatcgat aactgttttt
    45481 taatatgaaa ctaaaaattt gctgcatcgt ttaaaaagtt ataaaattta aaattccaaa
    45541 caccaaagat tatttcaaat acttggaatt tggaattttc tataattgga ttttttattt
    45601 aataagtatg cttctaaact tagggatgtt tttcaatgcc attccagtac cacgaactac
    45661 tgctcttaat gggtcttcag caatgtaaac tggtaagtct gttttttgag aaatacgttt
    45721 gtctaatccg cgaagcattg atcctccacc tgcaagataa ataccagtat tgtaaatatc
    45781 agcggctaat tccggtggtg tttgcgataa cgtttccata atagcatctt cgatacgttg
    45841 aatcgatttg tctaatgctt tggctatttc tcgataagaa acttccactt gttttggttt
    45901 tccagtcaat aaatctctac cttgaacgga catgtcatct ggtggtgttt ctaaatcatc
    45961 ggtggcagca ccaatagtaa tttttatttt ttctgctgtt ccctctccaa caaataaatt
    46021 gtgttgggta cgcatatagt aaataatatc attggtaaat acatcgcctg caatcttcac
    46081 tgatttatca caaacaattc ctccaagagc aataactgca atttcagttg ttccaccgcc
    46141 aatatcaaca atcatgtttc cttttggttg cataatatcg ataccaatac caatagcggc
    46201 tgccattggt tcgtgaataa gatatacttc ttttccgttt acgcgttcac aactttcttt
    46261 taccgcacgc atttctactt cggtaattcc agatggaata caaacaacca ttcttaaagc
    46321 tggtgtaaat aattttttct ttaaagcggg aatacttttg ataaataaac taatcatttt
    46381 ctcagaagca tcaaagtcgg caataacacc atctttcaac ggtcgtatgg ttttgatatt
    46441 ctcatgggtt ttaccttgca tcatgttggc ttccttacca acagcgataa tttttccaga
    46501 aatcctatca cgagctacaa tcgacggact gtcaattaca actttatcat tgtgtattat
    46561 caaagtgttt gcggtaccaa ggtcaattgc aatatcctcg gtcatgaaat caaaaaatcc
    46621 catacgattt ttaagggttt atagtttata aatttttaac gtacaaagtt aaacaaatta
    46681 atgtttaaaa tgacgtgttc ctgtaaatac cattgcaact ttattttcat tgcaataatt
    46741 aatgctcaat tcatctttga tagaaccacc aggttggata actgctgtaa ttccagcgtt
    46801 atgtgctagc tctacacaat cgggaaatgg aaagaacgca tcacttgcca taacagcacc
    46861 tttcaggtca aatccaaagt tgtttgcttt ttcaacagct tgttttaggg catcaactcg
    46921 tgaggtttgt cctgttccag aagcgatgag tgttccgttt tttgcaaaaa caatcgtatt
    46981 cgatttggtg tttttacata ctttcgaggc aaaaatcaaa tcttcaattt cttgttctga
    47041 tggtgccgta gtagttacaa cttttaaatc ttctctagaa tcggtaattg tatttcggtc
    47101 ttgaactaat aacccattca aacatgttct tacttgactg tttggtaatt caacatcgtg
    47161 ttgaaccaat ataattctat tctttttttc tgataaaata gctacagctt cttcgtcaaa
    47221 acttggtgct attaccactt cacaaaacaa attgctgatt tcttttgcgg ttgcgccatc
    47281 aatttttccg ttagcaatca atacgccacc gaaagcagaa gttggatcac cagctaatgc
    47341 cgcaacatag gcttctttca tcgtgtttct tgtagctaat ccgcaagcgt tgttgtgttt
    47401 taatatggaa aatgttggag cgttgttttt aaattctaaa attaaattca cagcggcatc
    47461 aacatcaagc aagttgttgt atgacaactc ttttccgtga agtttgctaa acattttgtc
    47521 aaattcgccg aagaagaatc ctttttgatg tgggttttcg ccatatctta aaatctgacc
    47581 ttctgaaata cttgctttga aatacgtttc atcctcgtta aaataattga aaatagccgt
    47641 gtcatagtgc gatgaaacat gaaacgcttt agtggcaaaa agttttcttt gctctaaaat
    47701 tgtagcacca ttttgactag aaataatatc taaaagagat gcatattcgt ttacagaagc
    47761 tacaataaca gtgtctttaa agtttttggc cgcagcacga attagagaaa ttccaccaat
    47821 atcaattttt tcaataatgt cagcttcact tgctccagaa gcaacagttt tttcaaacgg
    47881 atataaatca acgattacta aatcgatttg aggaatgtta aattcaacca tttgttgcac
    47941 atcgccttcg tggtcttgac ggtttaaaat cccgccaaaa acttttgggt gcaatgtttt
    48001 tactcttccg ccaagaatag acggataaga ggtaacatct tctacgggaa ctacagggat
    48061 tcctaagttt ttaataaaat cttcggtgcc accggtagag tagaggataa cattttgttc
    48121 atgtagtttc tgaacgatgg gttccaaacc ttctttggaa aacactgaaa ttagtgccga
    48181 ttgaattgtt tttgtagtgc tcattgtgtt gtgtgttata agcatacaaa agtagttttt
    48241 ttgaatgcat ttttcaacta atgagtgtaa caaatttcaa taattgtaca caaacaatta
    48301 ggaatagctt caggatattc catttatttt tttgaaattt acaaatctga tttaaaaacc
    48361 attaggaatg ttattatacc taagactgct aaaagagagt ttcctatttg ctatgaatgc
    48421 tttgcgaaac aataaactgc gcaccatgtt gtcacttctt ggggtaacta ttgggatttt
    48481 ttccataata gctgttttgg cggcagtaga ttctttagac aaaaaaataa aaggcgattt
    48541 gggtactttg gataaaaaca ctatgtattt aaaaagccaa tcttttggtc cttcagatat
    48601 tcctcgttgg aaaagagacc aatttcctga agtaaattat gaagaatttc agtacttaaa
    48661 atccaatttg aatgatgtag aaaacctttg ctaccaatat tttatgtttt ctagagaagc
    48721 tgtcaaaaat gatgggataa cggtttcgag tgtaaatgtg gttcccgtaa cttatgagtt
    48781 tgcagatatt caacggatgg aaattaacaa cggacgattt tttaatgaag ccgaatctaa
    48841 tgctgctgct ggtgtaattg ttttaggtta taatattgcc gaaggtttgt tcgataacgt
    48901 tgatccaatt ggaaaatcag ttcgtattta tggtcaaaat gtaaaagtaa ttggcgtaac
    48961 caagaaaaaa ggaatgagca tgggacttga agatagcgat gacgattcgg tttttcttcc
    49021 agtcaatttc ttaagaagat tgtatggtga caacagtaaa aactttcttc ctgtaattgt
    49081 tattaaacct gaaaaagatg ttgatattga cgctttaaaa ggcgaaatta ctcaaaaact
    49141 tcgtgcctat cgaggtgtta aacaaggcga aataaacaat ttttttcttg acattctttc
    49201 tggatttact gacatgatag atagtttcat tggtactcta aaaatgggtg gatggattat
    49261 ttctggattt tcgctcctag tgggtggttt cggaattgcc aacatcatgt ttgtatctgt
    49321 aaaagagcgt accaacctaa ttgggataca aaaatcattg ggcgccaaga ataaattcat
    49381 actctttcag tttctttttg aagccattat actttgtgta atcgggggga taattgggct
    49441 gttcttggtc tggataattg ccatggtgct caccgatatg ctcgactttg agtttgtgct
    49501 cagtgttgga aacatgctct tagggacttc tttagccgca gttatcggtc ttattgccgg
    49561 aatattacct gccatttctg cctcgcaact tgacccagtg gaagcgatac gaacggggat
    49621 gtaaagttta tgacttaaat ttaaagtata actaatagaa tctaattttc cgtatcctaa
    49681 tttgatacat caagcacgaa aaccgcttgt ttcaatagct ttaatgaatt ataattacaa
    49741 aaaaaacagc aattattttt tatatataat agactgcaac caaaagttta gacaaatttc
    49801 cacaaaaaaa gtaatgtaat ggtttttttg ttttgtgtgg ttggtggtat ttgattttga
    49861 agcaaaaaaa aagctccgca agtgcggagc ttttatattt taataaagaa tttgtgtttt
    49921 atttcaaccc gtcaatcttt gcttgaactt cttcgtcagt tccttcaaag ttttgaactt
    49981 cttctttacc gttagttaca gtagtgactg ttgccgtaac ttttccgtta tcattagatt
    50041 tttcaattct aacttctttt gaagtttctg ttgtcattgg agcagcttct gggttcatga
    50101 atttacaagt tgtacttgca catccttttt ccatacactc ttctttagac attgagttgc
    50161 attctgtcat ttctgcacat ccctttttag tgcattcttc aacgctcatt gtgccacatt
    50221 tttcgttaac cacacaacaa gaagccatag cagtatctga atgtcctgaa cctaagattg
    50281 gcgcaattac taatccaatt aaacaagtca atttgattaa aatgttcatc gatggaccag
    50341 aagtatcttt aaatggatca cccactgtat ctccagtaac cgctgctttg tgcgcatcag
    50401 aacctttata cgtcatttcg ccattgattt ctacacctgc ttcaaaagat ttcttagcgt
    50461 tatcccaggc accaccagca ttgttttgga atactgccca aagtacacct gaaacggtaa
    50521 ctccagccat atatccacct aacatttcag cgattagttg gttgttatct gcatacacta
    50581 atttacctaa aagaacgata gcaattggga aaccaatcgt taaaattcct ggtaacatca
    50641 tttctcttaa agctgctttg gtagaaatct ccacacattt accatattct ggtttacctg
    50701 ttccttccat gattcctgga atctctttga actgacgacg tacttcgtac accatgtcca
    50761 ttgccgcttt acctacagaa ttcattgcca aggcagagaa aactactggt atcattccac
    50821 caacaaatag catggctaat actggtgctt taaaaatatt aattccgtca attcctgtaa
    50881 aagttacata agccgcaaat aaagctaacg atgttaaagc tgccgaagca atcgcgaaac
    50941 cttttcctgt tgcggcagta gtgttaccta ctgagtctaa aatgtcagta cgtgttctaa
    51001 cttctttagg taattcactc atttctgcaa taccacccgc attatcagag attggtccga
    51061 aagcgtcgat agccaattgc atcgccgttg tagccatcat agccgaagct gctaaagcca
    51121 caccataaaa accagcaaat gcataagaag cccagatagc accagcaaat aataaaacgg
    51181 ttgggaatgt agaaatcata cccgttgcta aaccagcgat tacgttagtt cctgcacctg
    51241 ttgaagattt ttgtacaata gccaaaactg gttttgtacc aagacctgtg taatattcgg
    51301 ttactgatga aattacacca cctactacta aaccaacaat agttgcatag aatactcgca
    51361 ttgaagagat ttcttttaaa ccttcgccat agaaattcat ggtcattagt tctggtaaca
    51421 tgaattttac aaggaaataa caagagatag cggttaacac aatagaaacc cagtttcctt
    51481 tgtttaaagc accttgtact tgtgcttctt tcgcatcatc actagaaata ctaactagca
    51541 ttgtacctat aatagagaat aaaattccga aaccagcgat tgccattggt aataagatag
    51601 gaccaattcc gccaaaagcg tcagtaatca ttccacccat gtctttaatc acatagttac
    51661 caagaaccat agccgcaaga actgttgcca catacgaacc aaataaatcg gcacccatac
    51721 cagcaacgtc accaacattg tcacctacgt tatcagcaat tgtagcagga ttacgaggat
    51781 catcttctgg aataccagct tctactttac ccactaaatc ggcaccaaca tcggcagctt
    51841 ttgtataaat accaccacca acacgagcaa acaatgcaat agattcagct ccaagagaga
    51901 aaccagcaag tgtttcaaga acgattgtca tgtcttctgt agaagtccac acaccgttca
    51961 tgaagtatct gaagaatata ataaagaacg ctgttaaacc aagaactgcc aaaccagcaa
    52021 cgcctaatcc cattactgtt ccaccaccaa aagaaacttt tagtgcttgt ggtaagctag
    52081 tacgagcagc ttgtgttgtt ctaacattgg ttttggttgc aattttcatt ccgatgtttc
    52141 cagcataagc cgaaaaaatt gctccaaaaa taaaagcaac tacgataagg atgtgtgtag
    52201 ttggaactat aaaagaaatt ccagccaaca ctaaacttgc tccaataacg aagaaagtta
    52261 ataatttgta ctctgctttt aagaaagcta atgcaccttc gtagatgtaa tctgagattt
    52321 ctttcatctt gccgtctcca gcatcttgtt ttaaaaccca agatcttttg atagccataa
    52381 aagctaatcc aatcaacgcc ataactattg gcacataaat catcattgat tccataaata
    52441 taattttcgt tataaatttt atcgggtgca aaagtaacaa aactttaaca aataaaaaca
    52501 atataattaa cggaatattg aataaataac tttagtttaa aagattctaa gagaagatga
    52561 cctaatagtt tctatttctg ctttttaggt aatgatgcca aacaataaag gaagccaaag
    52621 ttcgataggg tttccattgg tttgaaattt gttccatttc ttgaagcgtt tgactatcgt
    52681 atagttcttt aaatgttttt ttaattgcaa tgtctccaaa aggaattata tcaggcaact
    52741 gtaaacaaaa cattagatag acatcaatac tccaattccc catgcctttc aattgaatta
    52801 gttcagctct tatagcagtt tcttctttgg ttgcaaaaga atcgaaatca attttatttg
    52861 ccattacttt ttgggcaaga tgttttaaat agattgtttt ttgtcgagaa acgccgcaat
    52921 tgcgtaagat ttcatcagta gctcctagta ttgaagttgg agttattgtt tctaaaacat
    52981 taactatttt ggcataacac gcttttgcag atgcaataga tacttgttgt tctaatataa
    53041 tatgacaaag aggtgcaaaa ccttcctctc gcttttggat aattggaacg ccaaagtttt
    53101 ctattatttt agcaagggtt ttgtctttag tggttaggta ttctaaagca attttcatta
    53161 gaaataaaat ccaaacgaac ctttgatggt gaaattatta gtatcgggca agtttctgta
    53221 atttgctctc catgcacaat caattcggaa tactttaaaa atgtttccaa taccagcact
    53281 atattcccaa tagggttttt ctggagcaac atagactaat ccagaagcat taatagctct
    53341 gttttcatca gatattgtgc cataaacacc acgaattcca ataatttctt ggaggtttaa
    53401 ttttcttaaa taaggtattc gggaaaatat atttccgtta aaattatgtt cccattgaaa
    53461 agtggcatat tcatccgtta taaactcata aaagttaaga ttgctaaagg tattcttaat
    53521 gatgaaatag gtttgattac ccggaacaac actcattaat cccaatggaa tttgaccaaa
    53581 agtttttcct aattccatag ttaggttaga tcttccaaaa gcacctacaa tcagtggttg
    53641 acggtaatac aattgcagtt tatgataatc aaaatcactg ccaaataagc ctttaaaccc
    53701 cgaactataa tttataaaaa ctcgactgta tgggctattt acttctctgc gttcaactcc
    53761 ataaccgatg gtttttctgt tgggagtaaa ctctagttga agattaatct ccgattgttt
    53821 tacctcactt tttatagtgg aaaaagtatc atcagtaaaa taatctaaac taaaggtttc
    53881 tgaagccgat gctaaagttc ggtaagaaaa tcctatttga aaggaaagat tttttatagg
    53941 ttcaatttcc gcttcaatag ttgatagatt aatattggtc aatttgccat tagaacctgt
    54001 ggtaaacaat cctgaagaag cataacttct gccaagcaca tcattagttg tagtaagact
    54061 agcaccgatt tgttctacat cacgtctatt tccaccagag atgatgaagc gatttttttt
    54121 atcaatcatc cattttccag acaagccata tttgaattta ttatcgtcaa atccataagc
    54181 tgtataacct tgcaaacgcc aagtgtcatt tggaccaaaa taagttcttc cacccacacg
    54241 aagacgtaca ccttctactt cattgtatcc aaaagttgaa aaaattggac catagtcaaa
    54301 attattcatc tcgatataac cacttcctaa aatagaaact aagttataca ttcgtttgaa
    54361 ttttttgacc gttttcagcg tatcgagcat tttataaatt ccttcctcat ctttattaag
    54421 gcgttcaaaa cgattttgtc gccaaaaatc atttgattta ttgtaaacag agttatcaat
    54481 aaaatttact tcttctctat aaaattcatc aggttttttt atgtcaaact tgaagttttg
    54541 atacaaggta gttcgcttac catagatgcc tttggagtct tcttttttgc ttaaagcaaa
    54601 atctgtcatc atgtaatcac gtgttggtaa aaaaatagaa tcattcaaaa catcaaactc
    54661 ttgttctaca taaatatctt ttacccagtt tatattagca cttttggtaa cggccatgtt
    54721 aattttcttg atggcaaacg tagtatcgtt aacccaaaaa tcgcctttaa aagttagttc
    54781 tccttttctt cttggataaa acacaatatt ataacaccat ttgttgtcaa caaatgcggt
    54841 gtcggctaat acatagttgt aaacatcaat tcctgttctt gataatggac tagtaaaact
    54901 cttgtcaaaa aaatataagt agttttcata aatgtcaaaa tcattgtata aatctttgat
    54961 aaatgtattt actccatcgc catttcctaa tcctgaattt ttattagccg ttaagatttc
    55021 tttgtttttc cctgctatat tgtctccata aacttctgaa agagattcgt ttataaaaat
    55081 tggaagatag gtttttccag taactcttga ggtgtcaacg tttttgaaaa caaattccat
    55141 tcctttgaat agtttgcttt ttatcatagc actatcaatg gtgttcatgt caaactctac
    55201 tttttcatat ttgttgtatt ggtattgttt gaacattttt aatccatttt ttcttcttcg
    55261 ctcccaaatt tttctaagaa tatctagggc aggattgttt tttttagaag tttttccaga
    55321 aaacacaaca acttctttta ggttttcgcc ttcacttaaa gtaataataa aattatagtt
    55381 gacttgtttt gtgagcacaa cttctttagt aggaaaacca acaaaagaaa ccaacaaggt
    55441 tttataattt tgatcagatt cgatataaaa tttaccatct tcattagaaa caacgccaat
    55501 atttgttcct ttaaaaataa tatttgcata aggaatgggt tgattagtat tgtcaactac
    55561 ttttccacta actttggttt gtgctgatat aagattagtt agactaatac taaagaaaaa
    55621 taaaaaaaat aattttttca tagcaaaaaa aacttcatta caatttgctg taatgaagtt
    55681 tcaaatatag ataattttat attatttgta ggttactttt tttactgctt taataacatc
    55741 ttcagcattt ggtaaccatt cttttaaaag tgttggcgaa taaggcgcag gtgtatccgc
    55801 tgttgtgatt ctttgaattg gtgcgtcaag ataatcaaaa gcacgttctt gaaccatata
    55861 ggtaatttca gaagcaacac ttgcaaaagg ccaagcttct tctaaaacta ctaatctatt
    55921 tgttttttta acagagttta taatggcatc ataatccata ggacgaaccg ttcttaaatc
    55981 aataatttcg caagagatac cttcttttga taattcttca gcagcagtta atgcttcttt
    56041 gatgattttg ccaaaagaaa ctatagttac atcggttcct tctcttttaa tatcagctac
    56101 accaagagga agaatgtatt ctccatcagg cacttcgcct ttgtcgccat acatttgctc
    56161 tgattccata aagattacag gatcattatc gcgaattgct gcttttaata atccttttgc
    56221 atcatagggt gtagaaggaa ctactacttt taatccaggt gtgttagcaa accaattttc
    56281 aaaagcttgt gaatgtgttg ctccaagttg tccagcagaa gctgttggtc ctcgaaacac
    56341 cattggcata gggaactgtc cagcagacat ttgacgcatt tttgcagcgt tattaattat
    56401 ttgatcaata ccaactaacg aaaagttgaa tgtcatgtat tctacaattg gacgacaacc
    56461 attcatcgca gaacccactg caattccggc aaaaccaagt tctgcaatcg gtgtatcgat
    56521 aacacgcttt ggaccaaatt catctaacat tccttttgaa gctttgtaag caccgttgta
    56581 ttcggcaact tcttcaccca ttaaataaat agattcatcg cgacgcattt cttcactcat
    56641 cgcttctgca attgcttctc taaattgtat cgttctcata tattatcgta ttctttcttg
    56701 aaaattgaat ggcaaaaata aaaatttaaa acgatataag aatatattgt ttatgataat
    56761 tgcttttaat ttatagcagc aaaatcaaaa ttaaattatt tgcatgaacg aaaacgattg
    56821 aaaactgaaa aaatattatg cgtgcataca aaaaatagaa atatatttta tacttttacg
    56881 tccagaaaat tttaattcaa ttttataatg aaaatattag tttgcatcag tcatgttccc
    56941 gatacgactt caaaaatcaa ttttgtaaat ggcgatactg aatttgacac aaacggtgtg
    57001 caatatgtca ttaatccaaa tgatgagttt ggattaacaa gagcaatatg gtttcaagag
    57061 caacaaagtg caaatgttac tgtgataaat gttggtggag ctgaaactga atctacattg
    57121 agaaaagcat tagccatagg cgcaaatgaa gcaattcgaa taaatgcaac accaaccgat
    57181 ggtttttttg tagccaaaca attagcagaa gttgcaaaaa atggtaatta tgacttaata
    57241 atttgtggaa aagagtcgtt ggattataat ggcggaatgg ttcctggaat gctggcaaca
    57301 ttattaaatg caaattttgt aaattcctgt atcgatttaa aagttgatgg aaattctgcc
    57361 aaagtaatta gagaaattga tggcggaaaa gaaactcttt ctacttcact gccattaatt
    57421 attggtggtc aaaaaggatt agtggaagaa aaagatttac gcattccaaa tatgcgaggc
    57481 atcatgacag ctcgaacaaa ggctttaaca gttttagagc ctattacttc tgacaacaaa
    57541 accaaatctg taaaatttga aaaaccagct tcaaagtcgg cagtaaaatt aatttctcct
    57601 gataatttgg atgaattaat caatttatta cacaacgaag ctaaggtgtt gtagttataa
    57661 gtgttacgtt ggttgttaac agttttcaag caatttttga gagcaaactt ttaacctact
    57721 aaacttttaa tcttttattt ataatctttt taaattaaaa aatatgtctt tattaatata
    57781 tgcagaatcg gctggaggaa aattcaaaaa agtagctttt gaattggctt cttatgcaaa
    57841 aaaagtagcc gaaacattag gaacaactgt tactgcagtt accgttaatg ctaatgatgt
    57901 ttccgaattg tcaaaatatg gtgtagataa agtgttgaaa gtaagcaacg ataagttgac
    57961 aaatttcaac gcaaaagcct atgcagatat tgtcaaacaa gcagctcaaa aagagggagc
    58021 aaaagtaatt ttactgtctt ctaccactga tagtttatat atggctccaa ttgttgccgt
    58081 aggcttagaa gcaggttttg cttcaaatgt ggtaggtttg cctctgagca cttctccatt
    58141 tcaagtaaaa agaaatgctt tttctaataa agcatttgca ataacggaaa ttgaatcaga
    58201 agtaaaagtt cttggtttag caaaaaactc ttatggtatc gttgaaagtg cttcctcatt
    58261 aacggaagag gatttttccc caagtttaaa tgaagctgat tttaatatta aagtagattc
    58321 tgtagataag agttcaggaa aaattagtat tgccgatgcc gagatagtgg tgtctggcgg
    58381 tcgtggtttg aaaggtccag agaactgggg aatgattgaa gagttagctt cagtacttgg
    58441 tgcagcaact gcttgttcga aaccagtttc agatttagat tggcgtccac acagcgaaca
    58501 cgtaggacaa acaggaaagc cagttgcagc aaatttatac atcgccatag ggatatctgg
    58561 tgctattcaa catattgctg gaataaactc ttcaaaagta aaagtagtta tcaataccga
    58621 tccagaagca ccatttttta aagttgcaga ttatggtatt gttggagacg cttttgatgt
    58681 tgttccgaga ttaacccaaa aattaaaaga gtttaaagca aataattcat aacgattaac
    58741 taaccttaat cagagtaagc tgttctattt tttgagcagc ttattttttt tgtagcaaat
    58801 ctttttcgtt actttgcaat tcgggttttt gatataattt gcaatccata aaagtttcat
    58861 tttgagaata caataattga agttgctgga ttaaattttc ataaactttt gaaatacaat
    58921 attttaaatt aaaagtaaaa aaataacgtt tgatgaataa caatttttga tattttagaa
    58981 aaaatttgta caatgcgatg ttaaattatc ttttatatg
//
LOCUS       DAOOBH010000004        57938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_647_length_57938_cov_8.069243, whole genome shotgun sequence.
ACCESSION   DAOOBH010000004 DAOOBH010000000
VERSION     DAOOBH010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 57938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57938
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_647_length_57938_cov_8.069243"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1111
                     /locus_tag="PK218_01050"
     CDS             <1..>1111
                     /locus_tag="PK218_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPW97129.1"
                     /translation="DVYIPFINLNHALDGDKVKAYVYNRRSSRKPEAEVIEILERAKT
                     EFVGVIDIQKNFAFVSTANAKMYTDIFIPKNKYNDAENGDVVLVKLEDWPAKADSPFG
                     SVIKVLGKPGEHNTEIHAILAEYGLPYDFPIEVDAFANKLDTSITQEEIAKRRDMRDV
                     LTFTIDPKDAKDFDDALSFQVLENGNYEIGVHIADVSHYLKEGTILDDEAYNRATSVY
                     LVDRVVPMLPEVLSNFACSLRPHEEKYTFSAIFQLNPKAEVVDSWFGRTVIYSDQRFS
                     YEEAQSIIESKSDVIPAEISLTGNEYKVDAKIVEATLKLDELAKILRRKRMAAGAISF
                     DKVEVKFNLNEQAEPVGVFFKISKDANHLIEEFML"
     assembly_gap    1112..1211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1212..2029
                     /locus_tag="PK218_01055"
     CDS             <1212..2029
                     /locus_tag="PK218_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPW97130.1"
                     /translation="FFKISKDANHLIEEFMLLANRKVAEFIGKQKKTFVYRIHDEPNE
                     DKLINLQTVISKFGYSINMKSKQDISKSLNNLLNEVSGKKEQNLVDTLTIRSMSKAKY
                     STDNIGHYGLAFDYYSHFTSPIRRYPDVMVHRLLQYYLDGGKSASAEVYEEKCEHSSN
                     MEALAAQAERDSIKYMQVKYMQDHKDQEFLGVISGVTEWGIYVEIVENKCEGMVRIRE
                     IKEDYYTFDEKQYALVGEMSKGLLQLGDEVYVKVKNADLVKKQLDFHFIRRKE"
     gene            2082..2747
                     /locus_tag="PK218_01060"
     CDS             2082..2747
                     /locus_tag="PK218_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPW97131.1"
                     /translation="MKKIVFSLLIFSSIAFGQVHKNLGDFDKVTSFDKIDVMLIPSSE
                     NKIVIDGKEANEVELVNKNGELKIRMPFTKILSGDNISVTLYFKKINAVEANEGSRIA
                     CNDEIKSTSFDVKAKEGSEVKLILNVQKLTLRTANGSKVTLEGFATNQDVLVNSGGIY
                     QAKNLKTEQTTITSNAGGEAAIYATVFVDAKVRAGGEITIHGKPKEINQKVVAGGTIE
                     EAK"
     gene            2804..3496
                     /locus_tag="PK218_01065"
     CDS             2804..3496
                     /locus_tag="PK218_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPW97132.1"
                     /translation="MIFLNDILSAIPLGFFLSFMIGPVFFVLLETSVVKGFRAAVMFD
                     LGVVLADIVFILIAFFSSYRLIQSIKDDPALFIFGGLVMLTYGIISFINNRKEAKKNI
                     DPEDPAELAKNDYFSLFMKGFFLNFINIGVLGFWLAILITIGPQLELKTSRMLTFFST
                     LIIVYFITDLFKIILAKQLRSKLNPKNILLIKKFISIVLIICGVVLLSQAWFPKERKI
                     VDKAFEKLEKQH"
     gene            3506..4162
                     /locus_tag="PK218_01070"
     CDS             3506..4162
                     /locus_tag="PK218_01070"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HPW97133.1"
                     /translation="MKKIIFLLFLFFSISFFAQNDTLSVVRHTDSDFIIPKKLKVIYK
                     ELPNEFFINVPNVKSFKVSGNGLFKKSNNLYYIDNREEDEIFINFEIILNNNQIKKER
                     HLIKKRSLSNLIIALNSQDGPIFKLQKEKLKNAVLELKFEDENIDVKKFMFIYKFKVK
                     IPGRQEIEVNGNKIDTKTFESIKKFTSRNDIITIYDVKTQIRCGISTTQIQPLVITIL
                     "
     gene            complement(4436..4506)
                     /locus_tag="PK218_01075"
     tRNA            complement(4436..4506)
                     /locus_tag="PK218_01075"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4472..4474),aa:Cys,seq:gca)
     gene            complement(4604..4963)
                     /gene="folB"
                     /locus_tag="PK218_01080"
     CDS             complement(4604..4963)
                     /gene="folB"
                     /locus_tag="PK218_01080"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPW97134.1"
                     /translation="MGTIKLQNIRTFSFHGCLMEEAKIGSDYRVDLEIKTDLRKSSLS
                     DELKDTVDYVLLNKIVVEEMAIRSNLLEHVAHRIIARIFGEIPEVSRIVLAVSKLNPP
                     IGGDVEAVTIEMEEYRS"
     gene            5047..>5345
                     /locus_tag="PK218_01085"
     CDS             5047..>5345
                     /locus_tag="PK218_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPW97135.1"
                     /translation="MSTEEKSLNFIEQIIEEDLKHGLSNDKLRFRFPPEPNGYLHVGH
                     ASAICLNFGLGLDYNSPVNLRFDDTNPAKEEQEYVDAIKRDIEWLGYEWANECYAS"
     assembly_gap    5346..5445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5446..6483
                     /gene="glnS"
                     /locus_tag="PK218_01090"
     CDS             <5446..6483
                     /gene="glnS"
                     /locus_tag="PK218_01090"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HPW97136.1"
                     /translation="ESDYLEQISHSFCTLEFLPHRELYDWFLDQIYDDKKVRPKQREF
                     ARRNLSHTVVSKRKLLQLVEEKHVTGWDDPRMSTISGMRRRGYPPMAIRNFANTIGIA
                     KRTNLIDVSLLEFCVREELNKTAPRVMAVLNPVKLVITNYPKGKTEILEAENSQEDAS
                     LGFREVPFSRELYIEREDFQEEAHKKFFRLTLGTEVRLKNAYIIKGESVIKDENENIT
                     EIHCTYDVDSKSGSGTEASQRKVKGTIHWVSIEHAIEAEVRVYDRLFTNENPDGNKEV
                     DFKEYINPNSLEIITGYVEPSLQSAKNLDQFQFQRLGYFCVDKDSSAEKLVFNKTVGL
                     RDTWAKVESKE"
     gene            6816..7802
                     /locus_tag="PK218_01095"
     CDS             6816..7802
                     /locus_tag="PK218_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97137.1"
                     /translation="MDKAALIKNLFLERKELSVTEIVNEINVSKQYIHRVIKTLLENN
                     EIIKIGMPPKTIYKLNDTEIVSIVKNEGDFSQDKIRFLDENFMIITEIGQILNGVEGF
                     QNWCLKRKLPLEKTVDEFILTKKKYTNYLDSNGLLSGLAKLKSTVGFKEIYMDELLYL
                     DFYAIERFGKTRLGTILHYAKQGQNIMLMKILIKEIRDKIDLIISTYKIDAIAFVPPT
                     IKRETQLMKVLANGLKINLPSVEILKISGIIPVPQKSLNKIEERINNAESTFVVKSNV
                     TFNTILLIDDAVGSGATLNQIAGKIKNKDVAKIVIGLAIVGSFKGFDVITDV"
     gene            complement(7854..8819)
                     /locus_tag="PK218_01100"
     CDS             complement(7854..8819)
                     /locus_tag="PK218_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPW97138.1"
                     /translation="MPFLIIILVLGLFILFASFFTVKQQSSFIVERFGKFQSIRNSGL
                     QLKVPIIDRIAGRVNLRIQQLDVIIETKTKDNVFVKLKVSVQFKVIQEKVYEAFYKLE
                     YPHDQITSYVFDVVRAEVPKLKLDDVFERKDDVAVAVKRELNDAMTTYGYDIINTLIT
                     DIDPDIQVKNAMNRINAADREKTAAEYEAEASRIRIVAKAKAEAESKKLQGQGIADQR
                     REIAKGLLESVEVLNSVGINSQEASALIVVTQHYDTLQAIGADTNSNLILLPNSPQAG
                     SDMLNNMVASFTASNQVGEAMKKANSTKRLSKRDSDDLDRNESEE"
     gene            8901..9545
                     /locus_tag="PK218_01105"
     CDS             8901..9545
                     /locus_tag="PK218_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PorT family protein"
                     /protein_id="HPW97139.1"
                     /translation="MNKLFTLIVLCCTTISFAQYGRRDGNRIGLSAGISQTSLFTNNF
                     KSSPEIGFAGGLSVRGNYYNNWSMIYGMQFFSSNFNLQSTFDQKIKYNLEGVKVRLLL
                     SYNVVEDHVSLDFGPVLQINGKLKVSDSDKNKILDGTLLRAEQIQDISTFSGNFYLGV
                     SAGSKVVRAVIFYEYGFTNILNNLNSEDGLKVLNNGDKFKGNLGTINGQVIINL"
     gene            complement(9864..>9986)
                     /locus_tag="PK218_01110"
     CDS             complement(9864..>9986)
                     /locus_tag="PK218_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97140.1"
                     /translation="MQPLRLSLVGALKGPHLFDIIEMIGKEETIKRIGKAITTL"
     assembly_gap    9987..10086
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10087..10670)
                     /locus_tag="PK218_01115"
     CDS             complement(<10087..10670)
                     /locus_tag="PK218_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPW97141.1"
                     /translation="MSRPVRVRFAPSPTGPLHIGGVRTALFNYLFAKKHNGVFYLRIE
                     DTDQNRFVPGAEAYIFEALEWLGIAPSETVGKNEKFGPYRQSERKHLYKKYADELIEK
                     GWAYYAFDTAEALDFHRKQHEEQGKTFIYNWHNREKLDTSLVISKEETEKRIANGEAY
                     VIRFKTPVNETLHLKDIIRGDINFETNLLDDKVL"
     gene            10813..14220
                     /locus_tag="PK218_01120"
     CDS             10813..14220
                     /locus_tag="PK218_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97142.1"
                     /translation="MNNSNIIYEKLESFIKKFYTNELLRGTLFFIGLGLLYFIFTLFI
                     EYFLWLKPTARTILFWTFIVVELFLLFRFIAFPLFKLFKLQKGIDYNEASKIIGNHFS
                     EVGDKLTNFLQLSQDKNKSELLLASIDQKANTLQPIPFSNAINFGTNKKYIPLAIIPI
                     LFFAFFYLSGNSNFISQSLNRVVNYKQQFLPPAPFEFVVLNQSLQTEQNKDFLLKVKT
                     VGKIVPENATIVVGDESYFMEKGELGEFQFVIPKPTEKLDFHLEANDIKSKEYELQVI
                     TVPSIANFEMVLNFPGYLNKKSETIKGTGNAIIPEGTKVTWKMNTVATQKVDWLSDNI
                     KQTFAKTDNIFSLSKNINQNTEYQILTSNNKVANYEKLNYQISVIKDQFPTITVNTAP
                     DSLKVTKNYLLGQVSDDYGLTKLQVIYYPKDKPNDAKHGTIAVKKDSYDQFVFSFPST
                     LPVEEGVTYEYYFEVFDNDALHNYKSTKSTVFSNRIETQTEKEDQMLQQQNDNINALS
                     KSLKAQDKQLSEMDKLQKMGKEKDNLEYKDQQKVNEFLQRQKQQDEMMKEFAEKMKDN
                     LDKFKSEEKDEKKEALQERLEKSKEEIEKNQKLLDELKKLNDKISKEELFEKMEKFQK
                     ESKNQTKSLEQLVELTKRYYVEKKAEQIADKLNKLAEKQDKLSEKNEENNAAKQDEIN
                     KEFDKIQEELNQLEKDNKDLKSPMDLPKTDEKEKSIDEDLKNAKDQLQKQQKDKAKPK
                     QKNAAKKMKQMSQEMSESMASGEMEQMEEDVAMLRQILDNLLAFSFSQEDVMKQFKGL
                     KRGAPSFNKHLKTQQNLKQQFKHVDDSLFSMSLRNPKIAEDVTKEIGNVQYNVDKALE
                     NLAEAIIIKGNSHQQYAVASSNKLADLLSEILNGMQMSMQMSGAGKGKPKPGQGKGEM
                     QLPDIIQKQDGLGEKMKKGMKKGEKPGEGEEGQDGEPKNGSQGNKEGQGQGKDGKGKN
                     GKNGQSGGSGTSQGNKDGENGQDGEGDAQMIMEIYKEQQQVREALQNELNKQGIGGNG
                     QGTLDKMKEIEKQLINKGFKNETLQKMLNLKYELLKLEKAIQQQGEDKKRQSETNKKE
                     FNNQVNALPAKLQDYLNSIEILNRQSLPLRSNFNQKVQEYFKNNDKL"
     gene            14207..14626
                     /gene="ybeY"
                     /locus_tag="PK218_01125"
     CDS             14207..14626
                     /gene="ybeY"
                     /locus_tag="PK218_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPW97143.1"
                     /translation="MINYNYEIDFQLDNEENYSFWIENVINSENKILGEVNYIFCDDD
                     YLLEINKQYLNHDYFTDIISFDYTEGNLISGDIFISIDRVKENANDFKVSFEDELKRV
                     IIHGILHYCGYKDKSLADEKMMRSKEEEKIKMFHVKH"
     gene            14677..16548
                     /gene="mnmG"
                     /locus_tag="PK218_01130"
     CDS             14677..16548
                     /gene="mnmG"
                     /locus_tag="PK218_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPW97144.1"
                     /translation="MFLEKYDVIVVGAGHAGCEAAASAANMGCSTLLVTMSLQNIAQM
                     SCNPAMGGIAKGQIVREIDALGGYSGIISDNTAVQFKMLNKSKGPAMWSPRVQSDRMR
                     FAEEWRLRLEQTPNVDFYQEMVKGLLIKNNKIEGVVTSLGIEIKAKTVILTNGTFLNG
                     LIHIGDKQFGGGRAGESAAFGITDDLVKAGFEAGRMKTGTPPRVDGRSLDYTKMREEP
                     GDDKPAKFSYSDETKPLTNQKLCHMTYTSLEVHDILREGFERSPMFNGRIKSIGPRYC
                     PSIEDKINRFADKERHQLFVEPEGWNTVEVYVNGFSTSLPEDIQYKALRSVAGFEKVK
                     FFRPGYAIEYDYFPPTQLKHTLETKLVEGLYFAGQINGTTGYEEAASQGLMAGINAAL
                     KVKEEQPLILRRDEAYIGVLIDDLITKGTEEPYRMFTSRAEYRTLLRQDNADYRLTPK
                     GYEIGLASEKQLRRMEYKFNESEKMVDFFKETSISPEEANPILIEKGSSTMSQSDKMF
                     KVFSRPQIELEDFIRFDKVKNYINDNNLDDEIVEQAEIQVKYSGYIEKERNNAEKLIR
                     LEDIKIPENFDYNKIKSMSIEAKQKLSKIRPITISQASRISGVSPADVSVLLIHMGR"
     gene            16726..17571
                     /locus_tag="PK218_01135"
     CDS             16726..17571
                     /locus_tag="PK218_01135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW97145.1"
                     /translation="MSFHNNIFYQKTKDYSVSKEVFDLHHNPEFDMLLTFPKPSLEAL
                     PRYYESEDYISHTDGKRSLFEKVYQIVKGIALRKKLKLINTISEKGKLLDIGAGTGDF
                     LSVAKKNNWNVIGIEPNKKAKSIATSKGVSLANDLKTLDNNSFDVITMWHVLEHVPNL
                     EEYISELKRLIKPTGTIIIAVPNFKSFDANYYQEYWAAYDVPRHLWHFSKTAIQKLFA
                     KQELNLVDVLPMKFDSYYVSLLSEKYKNGKMNFLKALRIGWKSNQYGKRKNEFSSHIY
                     IIKNK"
     gene            17704..18291
                     /locus_tag="PK218_01140"
     CDS             17704..18291
                     /locus_tag="PK218_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPW97146.1"
                     /translation="MKKSLIVLAIVFTFIACEKTTQTKEFKTAYIDTAKLMDESIEGK
                     DVEAKYKEKAKVMGSQLDAEVARWKDEAANFQKNAQANGQAWAQQKGAELQKREQQIS
                     YAQDGMLRQLQQESSVEMDSLVKSYKKIIKEYGKEKGFDYIYGTGEANSVLYAKDQYD
                     ITKEIIKMVNDKYKAADKKEVKADDKSEAKKADKK"
     gene            18401..20674
                     /locus_tag="PK218_01145"
     CDS             18401..20674
                     /locus_tag="PK218_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW97147.1"
                     /translation="METLSAEANYVLQFINQTNRSIFLTGKAGTGKTTLLKEIINSTH
                     KNCVVVAPTGIAALNAGGVTIHSLFQLPFGGFIPDSSANAHFSSQIKFETKATLNRHF
                     KMSASKKAVIQNMELLIIDEVSMLRADLLDAIDYMLQSVRRKKQAFGGVQVLFIGDLL
                     QLPPIIREEEWKTLRNYYSGKFFFHSHVVQTNPPLYVELSKIFRQTDEIFIDLLNNLR
                     NNTITSKNISFLNQFVQPNFNLKANKGYITLTTHNAKADAINEQSLKDLEGTSVKFKA
                     NIVDDFPDKIFPLEEYLELKVGAQIMFIKNDLSFEKNYFNGKMGFIKSLSNDEIWVHF
                     PDENKTIEVERYEWQNIRYFVNDNTKEIEEEVLGTFVHFPIKLAWAITVHKSQGLTFD
                     KAALDVSQVFLPGQAYVALSRLRSLNGLILLSPIQMNGISNDDDVMNYSANKASTDVL
                     QSELQKQTKLFLHHYLKNSFDWTELAQEWRNHKFSYLDSDLSAKSKHKNWADEQLDFC
                     EALLDPAKKFRSQLDKIFNDDKVDFKFINERIQAAYGYFFEKIDTLEYQILYKIEEIK
                     RIKQVKQFYQELQVIEELQTNAVLRLMKAKKLIENVMNETILDKQSLNSSEIKNYKRI
                     KTERVIEDFKKKNINLIEEQEDVSYYQPKKKKSEKEAKKPTTQITYELWVDKNSIEEI
                     ALIRKLTVGTILTHFTKLVQDKAVAINDVLPEDKLKSLSKLFENYNEETLNTMKEQVG
                     EDFSWNELRLFKASLNV"
     gene            complement(20671..21057)
                     /locus_tag="PK218_01150"
     CDS             complement(20671..21057)
                     /locus_tag="PK218_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPW97148.1"
                     /translation="MNNPASLLILVFLAITFIQSAYDKIFDWKANVEFLKVHFSKTSI
                     LKNYVAQSIVLLVFLELCTGILTLVGCAQLLINGGRTFGFYGAVLSSITLILMLFGQR
                     LAKDYDGARTIAIYFIPSVLAVYWLN"
     gene            complement(21062..22210)
                     /locus_tag="PK218_01155"
     CDS             complement(21062..22210)
                     /locus_tag="PK218_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPW97149.1"
                     /translation="MHFTEIIGQDYIKNHLIKSAVAGRIPHAQLFVGPEGSGTLAMAI
                     AYAQLVLCKNTSQDNSEGNQSCNLKFQSLSHPDLHFIFPNTSNDEVKSKPKSADFLAD
                     FREFTLANPYGSLFDWYKHLGVKNKQGEIRVEDATDILKALALKSYEGGYKIMIVWMA
                     EKMNIAASNKILKLLEEPPEKTLFLLIAEDEEAIIQTIRSRCQVLHFGALPEKIIAAS
                     LVNRFQVETNLADKIAHQSQGNYNKAMHFLKHDEDELPFEEWFVHWVRSAFKAKGNAT
                     AIYDLIAWSDSIASIGRESQKQFLNYCIVMFRQALLHNYETKELVYVEPQTSGFTFDK
                     FAPFVTGNNISEIFESLSDAIYMIERNGNAKIVLTDLSIKLTRLIHKK"
     gene            22511..24364
                     /locus_tag="PK218_01160"
     CDS             22511..24364
                     /locus_tag="PK218_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97150.1"
                     /translation="MEKNNHLSLLIFLLLTIFSHQLSAQLNVPFKVRHQSFVKGDMTI
                     IANNIVNRKDSFVKAVAPYNSTDYSAKHNDELNMEYIDIDNDNSTFSSSNANFSTEKT
                     ANLKVVYAGLYWSATYKYNIGKQKRNRKYVAVDKDRDAFDVVKLKLPRQEKYIDIKGE
                     ILFDGLNENGFEENAPYAVYADVTKLLSDLQNPQGNYTVANVRATNGTLSGGVSAGWT
                     MIIVYEDKQETGKFITTYDGFTGVSKESSDIDFNGFQTLPEGKVNLKIAGAGLEGDLG
                     LKGDQISFKSAENEDFTILNTKVRDFNNFFNSSITINDEVFAKRFPNSQNTLGYDSFL
                     MTVYNPDNQVIGNNTTEAKLRFKSTGDRYFMFFSAFSVEVLETAKAIDEIKPNELLVE
                     NKTLPTATNTTSLSNAVALNETTENTIQKEEVKEVTQYKITATKEKEEKSQTTTKTND
                     NVVATKTVKTTTKKADKIRVTPIESKSRSFPGEEKGYYIIANVFAKHSNATRFVSKLQ
                     SLGIEAGYFINQANNYRYVYVSHHENFSEAQTLYFSNINEKYFGDLWIMTVNKENDKI
                     VKNKIENTTFAKDKDVALTIKNTTIKKLIKSSLSFFEESLNYICHFKQKES"
     gene            24399..25586
                     /locus_tag="PK218_01165"
     CDS             24399..25586
                     /locus_tag="PK218_01165"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPW97151.1"
                     /translation="MKTINDFNFKNKKAIIRVDFNVPLDEKFNVTDVNRIEAAKPTID
                     KILIDGGSVILMSHLGRPKGKEEKYSLKHIIATCSKVLGVNVKFAEDCRGAIAKEAAS
                     NLQKGEVLLLENLRFYPEEEAGDVEFAKELASLGDIYVNDAFGTAHRAHASTTIIAQF
                     FPENKCFGLLLAKEIESLNRVLNNSVKPVTAVLGGSKVSSKITVIENILDKIDHMIIG
                     GGMTFTFVKALGGKIGNSICEDDKLDLALEILQKAKEKNVQIHIPVDVVAADAFSNDA
                     NTKIIDVNQIPDGWQGLDAGPKSLENFKKVILDSKTILWNGPLGVFEMENFANGTIKL
                     GEFIAESTKNGAFSLVGGGDSVAAVKQFGLEDKMSYVSTGGGAMLEMLEGTVLPGIAA
                     ILE"
     gene            25664..27931
                     /locus_tag="PK218_01170"
     CDS             25664..27931
                     /locus_tag="PK218_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPW97152.1"
                     /translation="MNKLKITSLAIVLLTSIGFAQDTIITAQVKKEPLTRQQYLDSIK
                     GTFVRDNIASCIDSMWMKELANLDLYDELSLDIQNIDVDQKVDYELPTELLKEQLKKL
                     NQKSAFNIEYNQGLENVIKSFLKNRRKSFERLMGISQYYFPMFEESLAAQNIPLEIKY
                     LAVVESALNPRAVSRVGATGLWQFMYQTGKQYNLNIDTFVDERSDPLKASKAATQYMK
                     NMYAIFGDWDLVLASYNSGPGNVAKAIRRSGGKQNYWNIRPYLHKETQGYVPAFLATM
                     YIFEYHKEHGIVPNKAIANHFATDTVNIKSKMTFKQISDLLDVPVAQLQFLNPSYKRD
                     VVPFITGENHYLRLPIDKIARFVSNEEKIYAYVNYEDSLREKPFANNTAIAANDGNST
                     SRLKYHKVKRGDTLSELSESYGVSISEIKKWNKLRGNSVPLGRKLKIYTEEAVAAVEK
                     KSSKIDTTSVKETKSAIASNSKNYTEEKVVSFKDVVKYHKVKRGDNLGEIASKYDVSV
                     AEVKKWNKLKNNNINLGANLKIIKNERVVTVVKKEIKPEKINTTIEEETQVASNDEQE
                     NPEDFYVVQKGDNLFTIAKKFNVSLEDLKEWNNIENGTIQSGSKLVLNNASTAQNESV
                     ETPKTETKIVEYVVKSGDNLGTIAKKYNASISDIKDWNEMEDDNISVGSKLIVSKKLF
                     IVPTQIAKTSKTSKNQTIAQSKSEEVKHYLVKKGDSLFSIAKKYPGVTISDIKKWNGI
                     GNESIKPGMKLRING"
     gene            27971..28945
                     /locus_tag="PK218_01175"
     CDS             27971..28945
                     /locus_tag="PK218_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HPW97153.1"
                     /translation="MNKTLFLYLCFAFLTFSCQKKVDSLSQEKAAAINTISVIIDEQL
                     WNGEIGDSIRNKFASPVLGLPQEEPLFTIKQFPLKLFEGFSTGSRNMIIVKKENKSEF
                     KIGINEFSTPQNSFHISGKNTVQIIALLEEHSAEMIKRMRQTEIEACLKTIKDSLLNT
                     KTIKSKFDIDITIPGNYEYALTSRNFVWIKKEITSGNISLLIYQVPYQRIHKYHTVND
                     IVKVRDSIGKKYIHGRPRNSMMITEEAYFPYFSRIKFNNFDAYETKGTWQMKNDFMSG
                     PFINYALRDKANNRVLILEGFCYAPSKTKRDLMFEVESIIKSVKILKK"
     gene            28951..29397
                     /locus_tag="PK218_01180"
     CDS             28951..29397
                     /locus_tag="PK218_01180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW97154.1"
                     /translation="MATEWKIKRFQALSLEELYQILKLRSEVFVVEQNCVYLDIDDKD
                     QKALHVFGQIDEKMVAYARLFNRNDYFENASIGRVVTHPDFRANKIGHQLMKQAIEAI
                     DNHFGEKQITISAQLYLKKFYESHGFEQTSEMYLEDDIPHIEMKRN"
     gene            complement(29394..30209)
                     /locus_tag="PK218_01185"
     CDS             complement(29394..30209)
                     /locus_tag="PK218_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW97155.1"
                     /translation="MRLFYLFAFFLLTSVVHAQEEAVFSVYFDFDKSDLNAKQGEDLI
                     KFIKTIDSTRIEAIEIFGYTDDIGKEAYNFKLSTKRASMIQQKMIDSGIKNKIIVTIE
                     GKGRILIDDDVVENLPEKRSKNRRVDVVINLKPLPKIVIPGFYNTIQKNHVVGDHIYL
                     DNILFDRGSSKLDYKSKTALDKIAKLLLKYKNLQFEVQGHVCCTPQYQKEAIDRETRK
                     RELSTNRAESVYKYLVFKKIAKNRMTFKGYGNTVPLGKGTEYDRRVELVITKI"
     gene            complement(30209..31843)
                     /locus_tag="PK218_01190"
     CDS             complement(30209..31843)
                     /locus_tag="PK218_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPW97156.1"
                     /translation="MFPKIKKRYILPAMLTGFLFIGVSFKQDFFEIAKQIEIFTNVYK
                     TVNQNYVDDLNPAELMDKAIKSMLVEVDPYTNFFNEQDVIKFKINNTGEYTGIGAMIT
                     RKEDKLIIREPYKGFPADKAGLKAGDEIIQIGDVLIADFKDDASQLMKGAKNTKIDLK
                     YLRQGKAMTAQLILDEVEIKAVPFFGKIDEKTGYIVLSQFNKKASYETKEALEKLKND
                     GCTQIVLDLRGNPGGLLNEAVNICNLFVPKGEIIVTTKSKNEKYNNTYKTTREPIDTE
                     IPIAIIVDGKSASASEIVSGALQDLDRAVIIGSRSFGKGLVQRPFDMTYGTQVKVTIS
                     KYYTPSGRCIQALDYAKKDKNGKAIRTDEKNYTAFKTKNGRTVYDGGGILPDVVMEES
                     KTSTIAEALEKNDAIFNYVTNYYYKNPTLGDKIPTITDADLTDFKNFIKKEKININTD
                     TEIALKAMLEKAKKDNVDSAIASEYQQLLTAVQKSEETMIDKNAKEIKNLILDEIIKR
                     YQYKEGLYHYYIKNNAEVKKSVTLLNDKSAFSKILQ"
     gene            complement(31884..32279)
                     /gene="rnpA"
                     /locus_tag="PK218_01195"
     CDS             complement(31884..32279)
                     /gene="rnpA"
                     /locus_tag="PK218_01195"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPW97157.1"
                     /translation="MDYTYPKAEKLKSKKIIDLLFSEGKSVGKYPLRLVYIQKDFEED
                     IPLKMGVSVSKKYFKNAVDRNYFKRVLRECYRLNKQLLIDNLNQKYCFMFFYQSKDRL
                     EYAEINQKTIQLFEKFVNSLQENETKNEN"
     gene            complement(32282..32695)
                     /locus_tag="PK218_01200"
     CDS             complement(32282..32695)
                     /locus_tag="PK218_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97158.1"
                     /translation="MNFLKITILFLAFSFGFQNVSAQPIRFKTSSASFTDKDSKGKWN
                     DWSEFVDANVLITLDAKKNLITVNSNEVQSFMIKAYGEITDNDEVNIVPFECIDNKFS
                     KCTILIITKKKEGNRMQFYINYNEVKFVYNIYNVK"
     gene            complement(32774..34651)
                     /locus_tag="PK218_01205"
     CDS             complement(32774..34651)
                     /locus_tag="PK218_01205"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPW97159.1"
                     /translation="MKYFYLLLLLPIFCFSQTKHYWQQKVDYTMEVSMNINNYQYKGT
                     QKLVYKNNSEDTLRKVYYHLYNNAFQPGSEMDMRLQNIVDPDGRMVDKVKNVDGKEVK
                     ESRIAKLQPNEIGYLKISNFKQDKVAAKAIEKGTILEVTLNKPLLPGKSTCFTLDFDG
                     QVPVQIRRSGRNNSEGIELSMAQWYPKMAEFDNEGWHADAYIGREFHGVWGNFDVKIT
                     IDKNYILGGTGYLKNNNEIGCGYEDVGKSVKKDKNAKTLTWHFIAPNVHDFTWAADKD
                     YIHDKLVRPDGVTLHFLYKNNPKISEVWKQAEPLTSKIMDFYNQNVGKYPYKQYSVIQ
                     GGDGGMEYAMCTLVLGEGTLGGFVGLIAHEMGHSWFQHVLATNESKHSWMDEGFTSYI
                     EDWVMNELSSKKVENPFAGAYKSYINLANSGKEQPLSTHADRYDENRSYSIASYSKGE
                     VFLAQLEYLIGKENLRKTIKKYYSDFKFKHPTPNDIKRTAERVSGAHLGWYLIDWTET
                     LNTIDYGIKNVEAATDGVQRTGVSLERIGRTPMPIDVMVEYIDGSKESFYVPLEMLFF
                     EKENPTPSIKRTTLPSWNWGNPNYFFEIPKGKSAIKKITLDASGLMADVKRENNVYEA
                     K"
     gene            complement(34661..36244)
                     /locus_tag="PK218_01210"
     CDS             complement(34661..36244)
                     /locus_tag="PK218_01210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPW97160.1"
                     /translation="MIKKFIAIYLIALSYSAFSQISISENYKKQSLNELQLQRWSHLD
                     LEKDSIPGMSVDKVYSELIKDKKGSKVIVAVIDSGVDIDHPDLKSTIWTNKNEKVNNK
                     KDDDKNGYVDDIHGWNFLGEANNENLELVRMLKKPNDGSEEYKLALAEYEKKYNKVFP
                     LKETVDLFLNADKNVREALKKEDYTSEELNNMPSVKYELFQSKRIMQSFIEQSEGKNF
                     HQDLKEFKDYVYDQLNFNLNKEFDGRKIVGDNPNDINDKKYGNNVVYSKDRKDSLHGT
                     HVAGIIAQIRGNGIGGDGVAQNVEIMPIRAVPNGDEYDKDIALAIRYATDNGAKVING
                     SFGKDFSPHKEWVWDAIKYAESKDVLIVFAAGNDGKNIDTEPSFPADTKDKKTEIASN
                     VIEIGAINYNYGSKMVADFSNYGKTNVDVFAPGVKIYATIPEGKYKYEQGTSMASPNV
                     AGVAAMLRSFYPNLKASQIKNIIMQSGTPINFDVEVGDDHKLIPFSETCVSGKIVNAY
                     NAMKMAEQLSKEPKSNPKA"
     gene            complement(36318..37178)
                     /locus_tag="PK218_01215"
     CDS             complement(36318..37178)
                     /locus_tag="PK218_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW97161.1"
                     /translation="MKIYPIEAGNFKLDGGAMFGVVPKTLWNRTNPADENNLIDIAAR
                     CMLIEDGKRLILIDTGMGNKQSEKFFSYYSLWGNHSLDSSLQKIGFHRDDITDVFMSH
                     LHFDHCGGSVNWNKDKTGYEVAFKNAKYWTNENHWQWATKPNNREKASFLHENIIPMQ
                     ESGQLHFIEKPDSDFGFSDEMNFGIYYVDGHTEKMMIPHIKYNDKTIVFCADLLPTAG
                     HLPLPYVMGYDTRPLLTMPEKEKFLKTAAENNFYLWLEHDAHNEIITVENTEKGVRLK
                     EVFHCDEILR"
     gene            37339..40401
                     /locus_tag="PK218_01220"
     CDS             37339..40401
                     /locus_tag="PK218_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPW97162.1"
                     /translation="MNKIIFYFLIAFYSTTFYSQNNLEVLLKKVDSLEKSGNYKEVQL
                     VFERLKKENKNKNQLYYSAKFLFAKGMSMANNGNYDAAKENFSKAIQVAKKIKPSHNS
                     KQLLSRIYLELSYCYFDWEVALKQNTIGYNYTLKNLPKDTLLIDYLSDRGFIHKQLRN
                     YEQSILASEKALKLIAKLQPKKYDLLGDVHNNLGIAYSDLHLHNQSLYHYAKGLEYNI
                     KSNQKDKSDIVNSANNTIWESLDYGDEKKAKEILNFLNKNFYKWYNNKDFATTDVSAN
                     LQSWGLYFKSIYHLSNLRNEIYDKNYFEAKKHFDTIVSIFEKYPSERKKKDNTLLLSR
                     YEYEEIFKSKPLTDELNIKQHIDFNKRTIQIARRDESKHEELVACLKLAIAFNRYKKY
                     DDALKTIDEAKKIDETFFNASRFTIGVLEATVLNSLNKNEESRRVILKSYQKLLEKQK
                     KIKSLQHLKYADFKKFNSSTFIRNTINSATIYFKIYQKDKNKGDLLIANNLFFIASDI
                     FAEFYQKGKYNYHLNAFNKEIASGLLETQMLIDPNDKVKIKAILNRIENNSSQHLWNL
                     FELKNSQNLKIPASLIRKLNELVFEKNSLQEQLEKSKSSVELKNQLATLNKEIDKTQK
                     AINQKDASFQKFKSSDFDVDNVQKQLSSNQIVVKYLVMDKKTFAFTISDKSIQLVNLG
                     DTELLKSTVLQFTKSINSIDNNYTKISQNLYGKLIEPILKEKQVATIIFVPEDFLNAI
                     SFESLMNKNGKFIVQNYIVSYAYSLKIWDFLQQKQTANKDTHQFVSFAPNYNKTPNSN
                     QNRGLRRGNLFDLAEAKIEAQTINNMFNGRLFLNEKATKTNFLQSTTQFDFHHLAMHS
                     MLEDDYSNSSLVFTNNQKVYFDELYQLNFPSKMVVLSACSTGLGTQESGEGIMSLSRA
                     LAYSGVKSSVYSLWQVPDKETSEIMISFYQNLKKGQPKDEALANAKTTFISNNPMKKH
                     PFYWAGFVVNGDVSPIVSNSNWIIYIGIGLVILALIFFFRKKLFQFRQ"
     gene            complement(40381..41085)
                     /locus_tag="PK218_01225"
     CDS             complement(40381..41085)
                     /locus_tag="PK218_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97163.1"
                     /translation="MDNETLINKYFEKSLSEEEQKQFVLLMETNVDFAKEVSYQKGVK
                     KAITLNERSDLKKKLQSFETSKPKVKSFKIWYAAASIILLFGVGFYFTQNATSSIYDD
                     YYQSYPNVVAPTVRGEMKEDSKSEAFFEYDNGNYKKSLELFSKIYDSENVDYALFYKG
                     LSLMELEKTKDAIVTFKQFDLSKNNAFTPFVKWYLALSYVKENQKEKAIPLLKSLTET
                     DNPQQEMAKKLLSELE"
     gene            complement(41135..41677)
                     /locus_tag="PK218_01230"
     CDS             complement(41135..41677)
                     /locus_tag="PK218_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW97164.1"
                     /translation="MSKPNHLILLEQLKKGDEKTIHSIYVDNKNGFLLFANRYNLSSD
                     DLLDVYQDSIVALCENAKKGTIDSLESSITTYLFSIGKYMIYKKLKSKNINISIDYDH
                     HLPQVVEDEEEEQDEEEIKILRDNFKKLGEKCQKVLRLFYYEEKKLDAIQIELEYTNK
                     DVLKSQKSRCLKQLKDLIKK"
     gene            41810..43138
                     /locus_tag="PK218_01235"
     CDS             41810..43138
                     /locus_tag="PK218_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW97165.1"
                     /translation="MKTILTIVITLFTVCMSFSQETVGERVVKNAKDKTYQRGEQKSD
                     ETVDGALNKVEEGIKGIFKKKDKKKKQESAAENQETKDSGIQNSSSENSSTKSIKKNS
                     KFDFEPGQKQLGLDDFSTTNVGDFPLGWNTNSSAEIVTFDENNQKWLSITKDGFFLPE
                     FIKDLPENCTIEFDVFTRYVSNNILNYSFYINAVENSKKDLDNKYIQNGFNFNWRPVV
                     GSASYFVYENGEEVSKNEGLKVNAFLTNNPENPIIARVSIWRQKSRLRVYVNEDKILD
                     LPQAFDPKLKYNSFKFGAEYMNYSNGREQDDEFMVANVRYAVAGEDTRSKLITEGRFV
                     TNGILFDVNSDSIKPESGTVLKEIASILQENPTVKVKIIGHTDSDGDANSNLVLSKKR
                     AIAVKTALSTFYGIDGSRIETDGKGESQPLNKNANATEKATNRRVEFIKL"
     gene            43160..44377
                     /locus_tag="PK218_01240"
     CDS             43160..44377
                     /locus_tag="PK218_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97166.1"
                     /translation="MYKKIILIFILSVLGKANAQSFTDGIFTTTFGNISWTTETGTDY
                     PNGGMVYGDYKDKGTIIGSFDNNGKEVVGSFHNGAAEGKFVFITPYGKDNFFANGIKT
                     FAGNWGYSSNNKYSQNPDYEWKVTGKIGEKDAVKNVTNVWSGKWNTTDGAMYLMQVGN
                     KITGTYKGVGTVEATYNPSTRLLKGTFTNNNFNKTGYIEFYFEGNTFKGKWGWNTSMT
                     EGNWDGTKHVKNNKELSKNVLTNQLSNSSSKKILFRIQAKSIYLNEHGVNPSNVSLYG
                     FVGFELYKVTNNSRVEVLSFGNKSKYLFNTNENQAFVEGSTYIFPNQPTYYREYEISE
                     ADWNNNNIQFELKVFHHIKAEKFGSNRDGKYFSETLDFKLIKLNTNLVAKREYQKEFG
                     EYYGLLNYIITKL"
     gene            44383..45021
                     /locus_tag="PK218_01245"
     CDS             44383..45021
                     /locus_tag="PK218_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97167.1"
                     /translation="MKKLLFTLTILLLFSCNKAKESAQDAITGTIEETIENKTGTQVE
                     LPDADEMDNNAAYITYKSESKNYLDGKEKMQASVIFQKDNDGLSIALQGVGEGGKSFV
                     TTLSHVPENFKLPLKGKFAVNNRYDGKNASAVIMFMNVTDNGMMTSELPYEGEISITK
                     LSKDSIEFEINGKGGDASDAESPSNWKAISGSGKMISPIIMSYGIDKNNVLK"
     gene            45028..45498
                     /locus_tag="PK218_01250"
     CDS             45028..45498
                     /locus_tag="PK218_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97168.1"
                     /translation="MKTKLTIIIFLIQLALFSQNNTVSSGATATGATGSATYTIGQIG
                     YTTNTGSNGTISQGVQQAFEIVTLSTNDVPQIQLTVIIYPNPTVQNVTLFIKDFELTN
                     LKFQLFDIQGRIISNGKITQNETEIEMTSLASAQYFLKISDSNSEIKTFKIIKK"
     gene            45504..47336
                     /locus_tag="PK218_01255"
     CDS             45504..47336
                     /locus_tag="PK218_01255"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HPW97169.1"
                     /translation="MKLTIKIFFLFSCFLSLFSAFAQAPEKMSYQAIIRNASNTLITN
                     QAVGMRVSILQGSATGTEVYKELFNPNPQTNINGLVTVEIGSGIPLTGTFAGIDWANG
                     PFFIKTETDPTGGTSYTIVGTSQLMSVPYALYAKNSGNASWELNGNTASATNFIGTTN
                     DFDLFFKRNNVNAGRISENNTSIGFNALNPISSGDSNTAIGVQSMFSNTGGYRNTATG
                     FGSLRSNTSGFENTSIGYASLYNNITGNLNTSVGSNALNSNTIGSENSAFGNSSLSSN
                     TSGNLNTASGRSALASNTTGSRNVANGSRALYSNTYGFNNTAIGVFTLEFNTSGDNNT
                     AVGFESLKTNITGYHNTASGYQSLYSNTSGYRNTAFGYQSLYSNNGLYNVAIGYESLK
                     LNTSGYSNTAIGYNSMYSNVDGRFNTAIGLSALYNNVNGFDNVATGIQSLFNNISGDK
                     NTANGRESLSLLTTGNNNIGIGYLANVPTATGSNQVRIGNTAITYAGTQVAWSVTSDR
                     RWKENIQPSNLGLNFINDLKPVFYTRKNDETKKVEYGIIAQELEASLEKFGANTNGII
                     TKDDEGMYSVRYNDLLAPMIKAIQEQQAMIEELKAEIQTLKNKK"
     gene            47336..48064
                     /locus_tag="PK218_01260"
     CDS             47336..48064
                     /locus_tag="PK218_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97170.1"
                     /translation="MKTTLIALVSIFLFAFSCNGDKTEKEQLNSLEKEALEVTKEAGL
                     KAEDFKDKIDELLTLEMAAQASGLPETEATKKHNTTVMESVKYEWKSDRTKKVAINGQ
                     TINVPATNYIELSWVKNTTLQQFKHDYHNPTAEELQKASSAMDKKANELNAEGKATKD
                     QTDAANSIAKGSMSKFKVEEVPNLGDYAVFVNSGIMGVSTRDLKVFYKGLSFTLEVDL
                     SDNAANNDKKAIQTAKMIINQKLK"
     gene            48066..48461
                     /locus_tag="PK218_01265"
     CDS             48066..48461
                     /locus_tag="PK218_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97171.1"
                     /translation="MKKIIILFLLVIGFKANAQLKFWNTDASSNMPKFEVEYQTKTTI
                     FMKVGYDVKPLYVFNKTAPQVYNGDGRTKYQMTVETSDNIAHRKFEISYTFYRQTNRY
                     LGYIKATYTYKDKRPTKIFEENFETVVNP"
     gene            48483..48971
                     /locus_tag="PK218_01270"
     CDS             48483..48971
                     /locus_tag="PK218_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97172.1"
                     /translation="MKKIIILTIVFFTIQTQAQLVQGELKIDENSKAELSFKGNKAVN
                     LYADFRENKHKINFVFTSTDIPLNSDKKEVVQFVFSTTVFKNGKNIGTMKRSPMPFFP
                     GDMFMPIETFDFISILSNLQTNAEDKISEIPAGSYEIIIEAKPIGTKGNISSARLFLK
                     IN"
     assembly_gap    49049..49148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <49149..50819
                     /locus_tag="PK218_01275"
     CDS             <49149..50819
                     /locus_tag="PK218_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97173.1"
                     /translation="IYNLPITNIDFSNLTSLIVLYCYGSNLVEIDLSNNLFLNGLYCG
                     SPNLTYVNIKNGWPSASQGIQIFNSPNLSYLCGDESELGPIALSFATLYPNCNVGTYC
                     SFNPGGTFYTLTGTNRLDSNQNGCDVNDISIANLKFDVFSNTTGSFYTNTSGNFSIPF
                     QAGNYFVLPKLENPTYFNISPSQVNLQFPDQTSPYNQDFCITPNGVHYDLEITITRLK
                     AQIPGADVPYKIIFKNKGNQIQSGTITLNFNDAIMDFVSSNPVQTSSTTNQLIWNYSN
                     LNPFETRDIEFIMNLNSPMETPALNNGDRIQFTGNIQPENNNIVDDFPADNDIAANFN
                     VVNSADPNDITCLEGNSVGPNQIGKYVHYMIRFENIGTANAMNVVVKNLIDTSKFDIA
                     TLVPLYSNYDFVTKQTANQFEFIFENINLPFDDANNDGYLVYKVKLKNNLTVGTTFTN
                     QANIYFDYNFPILTNIESTTIEALSASNFEINDVVISPNPTSDFINIQFKNAADFKTE
                     IYDIVGKRIGNFKNTNQIDVSNFNSGIYIIKITDFESKTVKTQKFIKK"
     gene            50988..51317
                     /locus_tag="PK218_01280"
     CDS             50988..51317
                     /locus_tag="PK218_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HPW97174.1"
                     /translation="MIKVSDEASKKIVSMMQEDGFDATKDYVRVGVKSGGCSGLSYEL
                     KFDKELGENDKVFEDNNVKIAVEKKSFLYLAGTILEFSGGLNGKGFVFNNPNAQRTCG
                     CGESFSL"
     gene            51326..52774
                     /gene="sufB"
                     /locus_tag="PK218_01285"
     CDS             51326..52774
                     /gene="sufB"
                     /locus_tag="PK218_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPW97175.1"
                     /translation="MSKYTEDDLKVELENKEYEYGFYTELDSETFPIGLNEEIVRAIS
                     MKKGEPEWMTNWRIEAFRAWQEMTEPEWANVHYEKPDFQAISYYSAPKKADPNKTLDD
                     VDPELLAMYKKLGISIDEQKKMNNVAMDIVVDSVSVATTFKKTLNEKGIIFCSISDAI
                     KEHPELVRKYLGTVVPQRDNFYAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINQAG
                     TGQFERTLLIADKGSYVSYLEGCTAPSRDENQLHAAVVELIALDDAEIKYSTVQNWFP
                     GNKEGKGGVYNFVTKRGICEKNAKISWTQVETGSAVTWKYPSCILKGDNSVGEFYSIA
                     VTNNFQQADTGTKMIHLGKNTKSTIISKGISAGKSQNSYRGLVRISANADNARNFSQC
                     DSLLMGNNCGAHTFPYIESKNTSAKIEHEATTSKIGEDQVFYCNQRGIPTEKAIALIV
                     NGFSKDVLNKLPMEFAVEAQKLLEISLEGSVG"
     gene            52812..53561
                     /gene="sufC"
                     /locus_tag="PK218_01290"
     CDS             52812..53561
                     /gene="sufC"
                     /locus_tag="PK218_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPW97176.1"
                     /translation="MLSIKNLHASVEDKEILKGINLEIKAGEVHAIMGPNGSGKSTLS
                     AVIAGNETYDVTDGSVELEGEDLADLAPEERAHKGVFLSFQYPVEIPGVSVTNFMKTA
                     INETRKANGKEEMPANEMLKLIREKSELLEIDRKFLSRSLNEGFSGGEKKRNEIFQMA
                     MLEPKIAILDETDSGLDIDALKIVANGVNKLKNENNAVLVITHYQRLLDYIVPDYVHV
                     LMNGKIVKSGGKELAYELEKKGYDWIKNEFN"
     gene            53630..54946
                     /gene="sufD"
                     /locus_tag="PK218_01295"
     CDS             53630..54946
                     /gene="sufD"
                     /locus_tag="PK218_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPW97177.1"
                     /translation="MDLKEKLISSFMAFEESIDVNHELHDVRTSAIKNFENKGFPTKK
                     EEAWKYTSLNSIIKNDYSVFPKHENTIEFKDVKKFFIHEIDTYKVIFVDGKFSSYLSS
                     TTHDGLDVCLMSSALNKPKYKMIIDEYFNKIASKEESLTSLNTAFANEGAYINIPKSK
                     VVEKPIEIIYFSTGKENALMVQPRNLVIVGENAHVQIIERHQSLNENPVLTNAVTEIF
                     AQKRAIVDYYKIQNDMQSANLIDNTYIAQKQQSHASVHTFSFGGNITRNNLNFYHQGE
                     HIESTLKGITIIGDKQHVDHYTLVQHATPNCESHQNYKTILNDSATGVFNGKIFVEKE
                     AQKTDAFQQNNNILLSDKATINAKPQLEIFADDVKCSHGCTIGQLDETAMFYMQQRGI
                     PKKEAKALLMYAFSNEVIESIKIPELKQRITKIIATKLGVNLGFDL"
     gene            complement(54950..56377)
                     /locus_tag="PK218_01300"
     CDS             complement(54950..56377)
                     /locus_tag="PK218_01300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPW97178.1"
                     /translation="MKKTVLFFVLLLLVALAYLRIAIYPQLDLISGFSAKSVASGHFL
                     DNRSLKTIQNGDNDIPKVDWATNEIDETHQFVTSNVYGFKERKAIFREGLGATLINDD
                     FDVTKPYEVPKRTKISSNLPFPYGNLEQKDTVFANIDYEKLQNAVAIAFDKKGEKNKR
                     TRSVLVVYKDKIIAEKYAAGFNKNSRILGWSMTKSITGTYFGILQHQGKIDIMKPAPI
                     AAWQNDERKNITINDLLHMNSGLEWEERYDKICDATQMLFIDEDMTKSQIDKPLVGKP
                     NQTWNYSSGTTNLLSGILRQQFKTHQEYLDFWYSSLLDKIGMTSAIIETDMAGNFVGS
                     SYGWATTRDWAKFGLLYLHKGNWNGEQIFDENWAKYVATPTNTSNGIYGAQFWLNAGG
                     NFPDVPKDMYRCSGYQGQMVAIFPSQDLVIVRMGLTEDPEFDFNGFLREVVSIFYRKE
                     RKVKIQRAQRPKSCALCIYRSEFFV"
     gene            56468..57448
                     /locus_tag="PK218_01305"
     CDS             56468..57448
                     /locus_tag="PK218_01305"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HPW97179.1"
                     /translation="MNKVFDLLVSKNFIRKEQYQAISEYQKLELFSIRNELLFLLYIS
                     ILLFTTGVGIIIYKNIDTIGHTVLLFLLALLTVVCFYFCYKKSSGFSKQEVTFENPLY
                     DYLVLFTTILMCTFIGYFQYNLNFQNSNYSIATLVSAMAALAMAYYFDNKSALIIGIT
                     ALGSFVGLSLKIQTIFENTIFNETNLLVAGILFASLLLVWQWFSEKNRLKIHFSFVIL
                     TFALHLMAICCFAGSSQDIYWIFYLILLGAAIYYFYQKSITFKAISWYVFALFYGYIG
                     LNVLILRIIDAVKMDQIFEFLVFLTPFYVIGSIVLFIKMIKNFKKKTNVS"
     gene            57438..>57938
                     /locus_tag="PK218_01310"
     CDS             57438..>57938
                     /locus_tag="PK218_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97180.1"
                     /translation="MLASNFNTIQNHFLLEEMEKLQSQDFVSKAQLSEIKNNTKTTKT
                     NSNILMRIGFFLLGNFLISSILGFCALFLSVLGDQNAIAFCFLLASIGCIVASELIYD
                     KNYFAYGFDDSFILSITLFFCVALGLFTENVIAVLFVFVLISSFCSVRYVHVPSTFFA
                     LMGLIGL"
BASE COUNT        21054 a         8571 c         9313 g        18600 t
ORIGIN      
        1 atgatgttta cattccgttt atcaatttaa atcatgcact tgatggcgat aaagttaaag
       61 cgtatgttta caatcgaaga agctcaagaa aaccagaagc tgaagtaatt gaaattttag
      121 aaagagcaaa gactgaattt gttggagtta ttgatattca aaagaatttt gcttttgtat
      181 ctactgccaa tgcaaaaatg tataccgata tttttattcc aaaaaataag tataatgatg
      241 cagaaaatgg tgatgtagtt ttagtaaaac tcgaagattg gcctgcaaaa gctgattctc
      301 cttttggttc tgtaattaaa gttctaggaa aacctggcga acacaatact gaaattcatg
      361 caatcttagc tgaatatggt ttaccgtatg attttccaat tgaagtggat gcttttgcta
      421 acaaattaga tacttctata actcaggaag aaatagcaaa acgtcgcgat atgcgtgatg
      481 ttttaacgtt tactattgat ccaaaagatg caaaagattt tgatgatgcg ttatcttttc
      541 aggttttaga aaacggaaat tatgaaatcg gtgtgcacat tgctgatgtt tcacattatc
      601 taaaagaagg aactatactt gatgatgaag cttataacag agcaacttcg gtttatttgg
      661 ttgatagagt tgttccgatg ttaccagaag tgttgtcaaa ttttgcttgt tcgctacgtc
      721 cgcatgagga aaaatatacg ttttcagcca tttttcaatt aaatccaaaa gctgaagttg
      781 ttgattcatg gtttggaaga acagtaatct attctgacca acgtttttcg tatgaagaag
      841 ctcaaagtat cattgagtcg aaatctgatg ttattccggc cgaaatttca ttaactggaa
      901 atgaatataa agttgatgcg aaaattgttg aagctacttt aaaattggat gaattggcta
      961 aaattcttcg tagaaaaaga atggctgcag gagcaatttc ttttgataaa gtagaagtaa
     1021 aattcaattt gaatgaacaa gcagaaccag ttggtgtgtt ctttaaaatt tcaaaagatg
     1081 ccaatcattt aattgaagaa ttcatgctat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn ntgttcttta aaatttcaaa agatgccaat catttaattg aagaattcat
     1261 gctattagca aatagaaaag tggcagaatt tataggaaag caaaagaaaa cttttgttta
     1321 tcgtattcac gatgaaccaa atgaagataa attaattaat cttcaaactg taatttctaa
     1381 gtttggttat tcaattaaca tgaaatccaa acaagatatt tcaaaatcat taaataattt
     1441 attgaatgaa gtaagtggaa aaaaagaaca aaatttagta gatactttaa cgatacgttc
     1501 aatgagtaaa gctaaatatt caactgataa tattggacat tacggattag cttttgatta
     1561 ttacagtcat tttacatcgc caattcgtcg ttatccagat gttatggtgc atcgtttgtt
     1621 acaatattat cttgatggag gaaaatcagc atcagcagaa gtatatgaag aaaaatgtga
     1681 acattccagt aatatggaag cattagctgc acaagccgaa cgagattcta ttaaatacat
     1741 gcaagtaaaa tacatgcaag atcataaaga tcaagagttt ttaggtgtta tttctggagt
     1801 taccgaatgg ggaatttatg ttgaaattgt agaaaataaa tgtgaaggaa tggttcgtat
     1861 tcgtgaaata aaagaagatt actatacgtt tgacgaaaaa caatatgcct tagttggtga
     1921 aatgtcaaaa ggtttattac aactaggtga tgaagtttat gtaaaagtaa aaaacgccga
     1981 tttagttaaa aaacaattgg attttcattt tattagaagg aaagagtaac caatcctgca
     2041 aggtttttaa aaaccttgta ggtatattaa aataaaaatt tatgaaaaaa atagtattca
     2101 gtttattaat attcagttca atagcttttg gacaagtaca taaaaattta ggtgattttg
     2161 ataaagtaac ttcgtttgat aaaattgatg tgatgttaat tccatcttca gaaaataaaa
     2221 ttgttattga cggaaaagaa gcaaatgaag tagaattggt taataaaaat ggagaattaa
     2281 aaatcagaat gccatttaca aaaattcttt caggtgataa tatttctgtg acgttatatt
     2341 ttaaaaaaat caatgcagtc gaggcaaatg aaggttcaag aatagcgtgt aatgatgaaa
     2401 ttaaaagcac ttcttttgat gttaaagcta aagaaggttc ggaggtaaaa ctaattttaa
     2461 acgttcagaa acttacttta agaactgcta atggttctaa agttacatta gaaggttttg
     2521 ctaccaatca agatgtttta gtaaattcag gcggaattta tcaagccaaa aatctaaaaa
     2581 cagagcaaac cactattaca agcaatgcgg gtggcgaagc agctatttat gcaactgttt
     2641 ttgttgatgc taaagttcgt gctggtggag aaattacgat tcatggaaaa ccaaaagaaa
     2701 tcaatcaaaa agtagtagcc ggcggaacaa ttgaagaagc aaaatagatt ttattttatc
     2761 atataaattt taaattctaa attgcaatgt caaaacaata aacatgattt ttctaaacga
     2821 tattttatct gccattccac taggtttctt tcttagtttt atgattggac ctgtattttt
     2881 tgtattactc gaaaccagtg ttgtgaaagg ttttcgtgct gcagtaatgt ttgatttagg
     2941 agttgtttta gcagatattg tctttattct tattgctttt tttagtagtt atcgattgat
     3001 tcaaagtata aaagatgatc cagctttgtt tatttttgga ggattagtaa tgcttactta
     3061 cggaattatt tcttttataa ataacagaaa agaagcaaag aaaaatattg atcccgaaga
     3121 tccagcagaa ttagcaaaaa acgattactt ttcccttttt atgaaaggtt ttttcttaaa
     3181 ctttatcaat attggagttt tgggtttttg gttggctatt ttaattacga ttggtccaca
     3241 attagagtta aaaacttcga gaatgctaac ttttttctca actttaatca tagtttattt
     3301 tataactgat ttgtttaaaa taatattagc caaacaatta agaagtaaac tcaatccaaa
     3361 aaatatttta ctaatcaaaa agtttattag tatcgtttta attatctgtg gagttgtttt
     3421 attatcccaa gcttggtttc caaaagaaag aaagattgtt gataaagctt ttgagaaatt
     3481 agaaaagcaa cattaaaatt tgctcatgaa aaaaataatc tttctccttt ttttattttt
     3541 ttcaatatca ttttttgcgc agaatgatac tttatctgta gtaagacata cagatagtga
     3601 ttttattatt ccaaaaaaat taaaagtaat ttataaagaa ctaccaaatg aattttttat
     3661 aaatgttcca aatgtaaaat cttttaaagt ttcaggtaat ggattattta aaaaaagtaa
     3721 caatttatat tatatagata acagagaaga agatgaaatt tttataaatt ttgaaattat
     3781 attgaacaac aatcaaatta aaaaagaaag gcatttgata aaaaagagaa gtttaagtaa
     3841 tttaattata gctcttaata gtcaagatgg accaattttt aaacttcaaa aagaaaaact
     3901 taaaaatgca gtattagaat taaagtttga agatgaaaat atagatgtta aaaagtttat
     3961 gtttatatat aaatttaaag ttaaaattcc tggaagacaa gagatagaag ttaatggaaa
     4021 taagattgat actaaaacat ttgagtctat taaaaagttt acttcaagga atgatattat
     4081 aacaatttat gatgttaaaa ctcagattag atgtggaatt tcgacaacac aaattcaacc
     4141 tttagttatt acaattctct aatcaatttt ttctaaaatt tattacaaaa aaacctccaa
     4201 gtttccctga aggttttata gacatcgtcc ggattcgaac cggagtataa ggttttgcgt
     4261 attctttaat caattttttc taaaaattta ccataaaaaa acctccaagt ttccttgaag
     4321 gttttcgagg catcgtccgg attcaaacca gagtatcggt tttgcgtatt cttttaatca
     4381 attttttcta aaaatttacc ataaaaaaac ctccaagttt ccttgaaggt tttcgaggca
     4441 tcgtccggat tcgaaccgga gtagtcggtt ttgcagaccg gtgcctagcc gctcggccac
     4501 gatgccagtt ggacggcaaa tatataaaaa attccaaact taaaactcca aattccaata
     4561 tttaaaacta attcgttgga atttggaatt tatttattgg aatttaactt ctgtactctt
     4621 ccatttcaat agtaactgct tcaacatcac caccaattgg cggatttaat ttagaaacag
     4681 ctaatacaat tcgagaaact tctggaattt caccaaaaat tctagcaata attcgatgtg
     4741 ctacgtgttc taataaatta gagcgaatag ccatttcttc gactacaatt ttgtttaaaa
     4801 gtacataatc tacggtatct ttcagttcat cagacaacga agattttctt aaatctgttt
     4861 ttatttccaa atctactcgg taatccgaac ctatttttgc ttcttccatc aaacaaccat
     4921 gaaaggaaaa agttctaata ttttgtaatt taattgttcc cattttttga tttccttatc
     4981 aattttcaaa ttttcaaatt ttcaaattga ctaattaaac atatctttgc taaaattaca
     5041 aaaataatgt caacagaaga gaagtcttta aatttcattg aacaaattat agaagaagat
     5101 ttaaaacacg gtttatcaaa tgataagctg cgttttcgtt ttccacctga accaaatggt
     5161 tatttacatg ttggtcatgc tagtgcaatt tgtttaaatt ttggattagg tttagattat
     5221 aattcgcctg taaatttgcg ttttgatgat actaatccgg ccaaagaaga acaagaatat
     5281 gttgatgcta taaagcgtga catagaatgg ctaggttatg aatgggcaaa tgaatgttat
     5341 gcttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaaag tgattattta
     5461 gaacaaattt cacattcatt ttgtacgcta gaatttttac cacatcgtga attatacgat
     5521 tggtttttag accaaattta tgatgataaa aaagtacgtc cgaaacaaag agaatttgct
     5581 cgtagaaatc tttcgcatac agttgtttca aaacgtaaat tattgcaact tgttgaagaa
     5641 aaacacgtta ctggttggga tgatccaaga atgagtacca tttcaggaat gcgtcgtcgt
     5701 ggttatccgc caatggcgat tagaaatttt gccaatacaa ttggaattgc aaaacgaact
     5761 aatttgatag atgtttcact tttggagttt tgtgttcgtg aagaattgaa caaaacagca
     5821 ccaagagtta tggcagtttt aaatcctgtg aaattggtta ttaccaatta tcctaaaggg
     5881 aaaaccgaaa tattggaagc tgaaaattca caagaagacg caagtttagg attcagagaa
     5941 gtgccttttt ctagagaatt atatatcgaa agagaagatt ttcaagaaga agcacacaag
     6001 aagtttttcc gattaacatt aggaactgaa gtgcgtttaa aaaatgccta tattattaaa
     6061 ggtgaaagtg ttatcaaaga tgaaaacgaa aatattaccg aaattcattg cacgtatgat
     6121 gtcgattcta aatcaggaag cggtaccgaa gccagccaac gaaaagtaaa aggaaccatt
     6181 cattgggttt ctatagaaca tgctattgaa gctgaagttc gagtttacga ccgattattt
     6241 actaacgaaa atcccgatgg aaataaagaa gttgatttta aagaatacat caatccaaat
     6301 tcattagaaa taataacagg ttatgttgaa ccaagtttac aatcggctaa aaatttagac
     6361 caattccaat ttcaacgatt gggttatttt tgtgttgaca aagattcatc ggctgaaaaa
     6421 ttagttttta acaaaacagt tggtcttcgc gatacttggg caaaagtaga aagtaaagaa
     6481 taaatacatt ataagtttta taaatgaaaa tcccgaaaca cttcgggatt tttctattat
     6541 taggattaac tattataatt aaaataagta tcataaaaag ctattaaaag tagctttcat
     6601 actttcgatt taatcaaaaa ataaaaaagg ttaatgtaat ttgttatttg ataatacgaa
     6661 aatgtcttaa atcgatttat tttagttttt gatgaaaaat aatataaatt ttcaaaattt
     6721 agtttactgt ttatagttta ctgtaaacta taaagaattt atttgtatat ttgtttatag
     6781 tttactgttt atagtaaact ataaactatc aagtaatgga caaagccgca ttaatcaaaa
     6841 atctttttct tgaaagaaaa gaattgagtg ttaccgaaat tgtaaacgag ataaatgttt
     6901 caaaacagta cattcatcgt gttataaaaa cattattaga aaataatgaa attataaaaa
     6961 ttggtatgcc gccaaaaaca atttacaaac ttaatgatac tgaaattgta tcgattgtaa
     7021 aaaatgaagg cgattttagc caagataaaa tacgttttct tgacgaaaat tttatgataa
     7081 ttaccgaaat tggtcaaata ctaaatggag tagaaggttt tcaaaattgg tgtttaaaaa
     7141 gaaaattacc actagaaaaa actgttgacg agtttatact aacaaaaaaa aaatacacaa
     7201 actatctcga cagtaatggc ttactatcag gattagcaaa acttaaaagc acggttggtt
     7261 tcaaagaaat ttacatggat gaattacttt atcttgattt ttatgctatt gaaagatttg
     7321 gaaaaactag gttaggaact attttacatt atgccaaaca aggtcaaaac ataatgctta
     7381 tgaaaattct aatcaaagag ataagagata aaatagattt gattatttct acctataaaa
     7441 ttgatgcgat tgcttttgta ccaccaacaa tcaaaagaga aacacaatta atgaaagttt
     7501 tagctaatgg attaaaaatc aatttaccaa gtgtggaaat tttgaagata agcggtataa
     7561 ttcccgtacc gcaaaagtca ttaaacaaaa tagaagaacg tattaataat gctgaaagca
     7621 cctttgttgt aaaaagtaat gttacgttta atacaatttt attaattgat gatgctgttg
     7681 gctctggcgc gacgttaaat caaattgcgg gtaaaattaa gaataaagac gtggcaaaaa
     7741 ttgttattgg attagcaata gttggaagtt ttaaaggttt tgatgttatt actgatgtat
     7801 aaaaaaagag ccaaattttg actctttact ttttttaaat tagatacgat attttattct
     7861 tccgattcat ttctatctaa atcgtctgaa tcacgttttg ataatctttt cgtgctgttc
     7921 gcttttttca tggcttcacc tacttggtta cttgctgtaa aacttgctac catattgttt
     7981 aacatgtcac ttccggcttg tggcgaattt ggtaacaaaa ttaagttcga attggtgtca
     8041 gcaccaatgg cttgcaaagt atcataatgt tgtgtaacta caattaaagc tgatgcttct
     8101 tgcgaattga ttccaacact atttaaaact tctacacttt ctaacaaacc tttagcaatt
     8161 tctcttcttt ggtcggcaat accttgacct tgaagttttt tgctttcagc ttcagctttt
     8221 gctttagcaa caattctaat tctgcttgct tctgcttcat attctgcagc tgttttttct
     8281 ctatcagcag cattaattct attcatcgca ttttttacct gaatatccgg atcaatatct
     8341 gtaattaacg tgttgataat atcataacca taagtcgtca ttgcatcgtt caattcacgt
     8401 tttacagcaa cggcaacgtc atcttttctt tcaaaaacat cgtccaattt taattttgga
     8461 acttcagcac gaaccacatc aaatacatac gaagtaattt gatcatgcgg atattctaat
     8521 ttataaaacg cttcgtatac tttttcctga atcactttaa actgaaccga aactttaagt
     8581 ttaacaaaca cgttgtcttt ggttttggtt tcaataataa catctaattg ttgaattcta
     8641 agatttacac gaccagcaat tctatcaata attggaactt ttaattgcaa tcccgagttt
     8701 cgaatgcttt gaaatttccc aaatcgttct acaataaaag acgattgctg cttaaccgta
     8761 aagaacgaag caaaaagaat aaataaacct aaaacgagaa taataattaa aaatggcata
     8821 gtttatatga ttttgttgtt aataaagtag ttgtacgaaa tattgtttat gtttgttaca
     8881 aatttcaagc tacttatttt atgaacaaac tatttacgct tattgttttg tgttgtacaa
     8941 caataagttt tgcccaatat ggaaggcgcg atggtaacag aattggcctc tcggcaggaa
     9001 tttctcaaac ttctttattt accaataatt tcaaatcttc tcccgaaatt ggttttgctg
     9061 gcggtctttc cgttagagga aattattaca acaattggag tatgatttat ggaatgcagt
     9121 ttttttcgag taatttcaat cttcaatcta cttttgacca aaaaataaaa tacaatctcg
     9181 aaggtgtaaa agtacgtttg ttattaagtt ataatgtagt agaagatcat gtttcattag
     9241 attttggtcc tgttttacag attaacggaa aactaaaagt aagcgatagt gataaaaaca
     9301 aaattttaga tggaacatta cttagagctg aacaaattca ggacatttct acatttagcg
     9361 gtaactttta tttaggtgtt tctgccggaa gcaaagtagt tcgagcggtt attttctatg
     9421 aatacggatt taccaatatt ctaaacaacc tcaatagtga agatggatta aaagtgttga
     9481 ataacggaga taaattcaaa ggaaaccttg gaactattaa tggacaagtg attattaatt
     9541 tgtaattaaa cgtaactcgc taactagaaa acaacatcga gtgcagaata agcgtcactt
     9601 aataactatt aaacaacatt gagtgcagaa taaaccacac ttaataacta ttaagcgaca
     9661 tcgagtacag aataagcaac acttagtaac tattaaacaa catctagtaa ctaataaaca
     9721 ttactcaata acgaataagc aacattgagt acagagtaaa caacactcaa taactagtaa
     9781 acattacttg ataactaata atcaacatcg agtaagtaga aagcaaaact aaaaaatccc
     9841 gagcaatcgg gatttttatt tttttataat gttgttattg cttttccaat tcgtttgatg
     9901 gtttcctctt ttccaatcat ttcgatgata tcaaaaagat gcggaccttt caaagcacca
     9961 actaagctta gtcgtaacgg ttgcatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnaaca aaactttatc gtctaataaa ttggtttcaa aattaatgtc accacgaatg
    10141 atgtctttta aatgcaacgt ttcatttaca ggcgttttaa atcgtataac ataagcttcg
    10201 ccattggcaa ttcttttttc agtttcttct tttgaaatta ccaaagaagt atctaatttt
    10261 tcacgattgt gccaattgta aataaaggtt ttgccttgtt cttcgtgttg ttttctatga
    10321 aaatctaagg cttcggctgt atcaaaagca tagtaagccc aacctttttc tattagttca
    10381 tcggcgtact ttttgtataa atgcttgcgt tcactttgac ggtatggtcc aaacttctcg
    10441 ttttttccaa ccgtttcact aggcgcaatt cccaaccatt ctaaagcttc aaatatataa
    10501 gcttctgctc caggaacaaa acgattttga tcggtatctt caattcgtaa atagaaaact
    10561 ccgttgtgtt ttttggcaaa taaataatta aataacgctg ttcttacacc accaatatgc
    10621 aatggtccgg ttggacttgg tgcaaaacga actcttacag gtcttgacat ttttactaat
    10681 ttttggcaaa gatacaattg tcgttgtaat tgacattgct attaaaattg aaatttggtt
    10741 taatttattt ttaactttta ttctatctat aaatttgtac atttatcggt tattaacaaa
    10801 agaggtacaa ttttgaacaa ttcgaatatt atttacgaga aattagaaag ctttattaag
    10861 aagttttaca ccaacgaatt actacgtgga acattatttt tcattggttt gggtttgttg
    10921 tattttattt ttacgttgtt tatagaatat tttctttggt taaagccaac agcaagaacc
    10981 attttgttct ggacattcat cgttgttgaa ctctttcttt tgttccgatt tattgctttt
    11041 ccgttgttta aattattcaa acttcaaaaa ggaattgatt ataacgaagc ttcaaaaatc
    11101 attggaaatc attttagcga agtaggcgat aagctgacca attttttaca gctttctcaa
    11161 gataaaaaca aatcagagtt acttttagca tcgattgacc aaaaagcaaa tactttgcaa
    11221 cccattccgt ttagcaacgc cattaatttt ggaaccaata aaaaatacat tccgttagcc
    11281 attattccga ttttgttttt tgccttcttt tacctttcgg gaaacagcaa ttttatttca
    11341 caaagtttaa atcgtgttgt gaattataaa caacaatttt tgccacctgc accttttgaa
    11401 tttgtggttt taaatcaaag tttacaaacc gaacaaaaca aagatttttt attaaaagta
    11461 aaaaccgttg gaaaaattgt tcccgaaaac gcaacgattg tagttggcga tgaaagttat
    11521 tttatggaaa aaggcgagtt gggtgaattt caatttgtaa ttccgaaacc aacagagaaa
    11581 ctagactttc atttggaagc gaatgatata aaatcgaaag agtatgaact gcaagtaatc
    11641 acagttccat cgattgccaa ttttgaaatg gtgttgaatt ttccaggtta tttgaataaa
    11701 aaatcagaga ctatcaaagg aacaggaaat gccattattc cagaaggaac aaaagtaacg
    11761 tggaaaatga atacggttgc tactcaaaaa gtagattggt tatcagacaa tatcaagcaa
    11821 acatttgcca aaacagataa tatattttca ctttctaaaa acatcaatca gaatacagaa
    11881 tatcaaatac ttacttcaaa caataaagta gcgaattacg aaaaattgaa ttatcaaatt
    11941 tcagttatta aagatcagtt tccaacaatt actgttaata ctgcgccgga tagcctaaaa
    12001 gtgactaaaa actatctatt agggcaagtt tcggacgatt atggtttaac gaagttacag
    12061 gtcatttatt atccaaaaga caaacctaat gatgctaaac atggaacaat tgctgtaaag
    12121 aaagacagtt atgatcagtt tgttttttct tttccaagta ctttgccagt tgaagaaggt
    12181 gttacctatg aatattactt tgaagtgttt gataatgatg cgcttcataa ttataaaagc
    12241 acaaaatcta cggttttcag caatcgtata gaaacacaaa ctgaaaaaga agatcaaatg
    12301 ctgcaacaac aaaatgataa tattaatgca ttgtcaaaat cgttgaaagc tcaagacaag
    12361 caattatctg aaatggataa gttgcaaaaa atgggtaaag aaaaagataa tttagagtat
    12421 aaagaccaac aaaaagtaaa tgaatttctt caacgtcaaa aacaacaaga cgaaatgatg
    12481 aaagagtttg ccgagaaaat gaaagacaat ctcgataaat tcaaatctga agaaaaagat
    12541 gaaaagaaag aagctttaca agaacgctta gaaaaatcaa aagaagaaat tgaaaaaaat
    12601 caaaagcttt tggacgaact taaaaaactg aatgacaaaa tcagcaaaga agagttgttc
    12661 gagaaaatgg agaaattcca aaaagaaagt aaaaaccaaa ccaaaagttt agagcagtta
    12721 gttgaattaa ccaaacgcta ttatgtggaa aagaaagcgg aacaaatagc agacaaacta
    12781 aacaaattag ccgaaaaaca agacaaactt tctgaaaaga atgaagagaa taatgctgca
    12841 aagcaagatg aaatcaataa agagtttgat aaaattcagg aagaactaaa tcaattagaa
    12901 aaagacaata aagatttaaa atctccaatg gatttaccta aaacggacga aaaggagaaa
    12961 agcattgatg aagatttaaa gaacgctaaa gaccaattgc aaaaacagca aaaagataaa
    13021 gccaaaccaa aacaaaagaa tgcggctaaa aaaatgaagc aaatgagtca agaaatgagc
    13081 gagtcaatgg catcgggtga aatggaacaa atggaagagg atgttgctat gcttcgtcaa
    13141 atattagata acttactagc tttttcattt tcccaagagg atgttatgaa acaatttaag
    13201 ggtttaaaac gaggtgcacc atcgttcaat aaacatttaa aaacgcaaca aaatttaaaa
    13261 caacaattta aacatgttga tgatagttta ttttcaatgt cacttcgtaa tccaaaaatt
    13321 gctgaagatg taactaaaga aatcggaaat gtgcaataca atgttgataa agctttagaa
    13381 aatttagcag aagcaattat aataaaagga aattcacacc aacaatatgc agttgcctct
    13441 tccaataaac ttgctgattt gttaagcgaa attttaaacg gaatgcaaat gtccatgcaa
    13501 atgtctggag caggaaaggg taaaccaaaa ccaggtcaag gaaaaggaga aatgcaattg
    13561 cctgatatta ttcagaaaca agacggttta ggtgaaaaaa tgaaaaaagg aatgaagaaa
    13621 ggagaaaaac ccggcgaagg agaagaaggt caagatggcg aaccaaagaa tggaagtcaa
    13681 ggaaataaag aaggacaagg tcaaggaaag gacggtaaag gtaaaaacgg taagaatggt
    13741 caatctggcg gaagtggaac ttctcaagga aacaaagacg gcgaaaatgg acaagatggc
    13801 gaaggcgatg cacaaatgat tatggaaatc tataaagaac aacaacaagt acgagaagct
    13861 ttgcaaaatg aattaaacaa acaaggtatt ggcggaaacg gacaaggaac tttagataaa
    13921 atgaaggaaa ttgaaaaaca attaatcaat aaaggattta aaaatgaaac ccttcaaaaa
    13981 atgttgaatt taaaatatga attattaaaa cttgaaaaag ctattcagca acaaggagaa
    14041 gataaaaagc gtcaatcaga aacaaataag aaagaattta ataatcaagt taatgcttta
    14101 cccgccaaac ttcaagatta tttaaacagt attgaaattt taaatagaca aagcttacct
    14161 ttgcgctcca attttaatca aaaggttcaa gaatatttca aaaacaatga taaattataa
    14221 ttatgaaatt gactttcaat tagataatga agaaaactat tctttttgga tagaaaatgt
    14281 cattaattct gagaataaaa ttttaggaga agtaaattat attttttgtg atgatgatta
    14341 tctgttagaa attaacaagc aatacttaaa tcatgattat tttacggata taattagttt
    14401 tgattacaca gaagggaatt taatttcagg cgatattttt atttctatcg atagggttaa
    14461 agaaaacgcg aatgatttta aagtttcttt cgaagatgaa ttaaaacgtg ttattattca
    14521 tggaatacta cattattgtg gttataaaga taaatcatta gctgacgaaa aaatgatgag
    14581 aagcaaagaa gaggaaaaaa ttaaaatgtt ccacgtgaaa cattaaacga tagttttaaa
    14641 taaaacgttc cacgtgaaac aatacagaaa agaaaaatgt ttttagaaaa atatgatgtt
    14701 atagttgttg gtgctggtca tgctggttgc gaagctgctg cttcagcggc caatatgggt
    14761 tgttcaacgc ttttggttac tatgagtttg caaaatattg cgcaaatgtc atgcaatcca
    14821 gccatgggtg gaattgcaaa agggcaaatt gtgcgcgaaa ttgatgcgct aggcggttat
    14881 tccggaatta tctctgataa tacagcggtt caatttaaaa tgcttaataa atcaaaaggt
    14941 ccagccatgt ggtcaccaag agtacagagt gatagaatgc gttttgctga agagtggcgt
    15001 ttgcgattag aacaaacacc taatgttgat ttttatcaag aaatggtaaa aggtttgttg
    15061 attaagaata ataaaattga aggtgttgtt acttcacttg gaattgaaat aaaagcaaag
    15121 acagttattt taaccaacgg aacttttcta aatggattaa ttcacattgg tgacaaacag
    15181 tttggcggag gaagagctgg cgaaagtgca gcttttggta ttactgatga tttagtaaaa
    15241 gcgggttttg aagcaggcag aatgaaaacg ggaacaccac caagagttga tggtcgttca
    15301 cttgattata ctaaaatgcg tgaagaacct ggtgatgata aaccagctaa attctcgtat
    15361 tcagacgaga caaaaccctt aacgaatcaa aaattatgtc acatgactta cacttcatta
    15421 gaagtacatg atattttgag agaaggtttt gagcgatcgc caatgtttaa tggaagaata
    15481 aagagtatcg gtccaagata ttgtccttct attgaagata aaattaatcg atttgctgac
    15541 aaagaaagac atcaactgtt tgtagaaccc gaaggttgga atactgttga agtatatgta
    15601 aatggatttt caacatcttt gcctgaagat attcaatata aagcattgcg ttctgtagca
    15661 ggttttgaaa aagttaaatt tttccgtcca gggtatgcta ttgaatacga ttattttcca
    15721 ccaacacaat taaagcacac gttagagacc aaattggtag aaggtttgta ttttgctggt
    15781 caaattaacg gaactacagg ttatgaagaa gccgcttctc aaggattaat ggctggaatt
    15841 aacgcagcac ttaaagtaaa agaagaacaa cctttaatat tgcgtagaga tgaagcttat
    15901 attggtgttc tgatagatga tttaattaca aaaggaactg aagaacctta tagaatgttc
    15961 acttctcgag cagaatatcg tactttgttg cgtcaagaca atgccgatta ccgattgact
    16021 cctaaaggct atgaaattgg tttagcatct gaaaaacagc ttcgtagaat ggaatataaa
    16081 ttcaatgaaa gtgaaaaaat ggttgatttt ttcaaagaaa cgagcatttc tccagaagaa
    16141 gccaatccaa tattaattga aaaaggaagt tctacgatgt cacaatcgga taagatgttt
    16201 aaagtttttt caagaccaca gattgagttg gaagatttca tccgttttga taaggttaaa
    16261 aattatatta atgataataa tcttgatgat gaaattgttg agcaagcaga aattcaagta
    16321 aaatattcag gatatattga aaaagaacgc aacaatgctg agaaattaat tcgtttggaa
    16381 gacattaaaa ttccagaaaa tttcgattat aataaaatca aatccatgtc aattgaagct
    16441 aagcaaaaat tgtccaaaat cagaccaata actatttctc aagcctcaag aatcagcggt
    16501 gtttctcctg ctgatgtatc agttttatta atacatatgg gtaggtagag atattagact
    16561 gtagatttta gatttgtgta aattaaaacg aggagtttaa tttattttaa gtgaattttt
    16621 cgttgtatat gaatataaag tgaaataaaa tctgaaatct aatatctaaa atataaatgt
    16681 tccacgtgaa actaactttc aaaaagctta taaataaaga catcaatgag ttttcataac
    16741 aatatttttt atcaaaaaac aaaagactat tctgtttcaa aagaagtttt tgacttgcat
    16801 cacaatccag aatttgatat gttattgacg tttcccaaac caagtttgga agcgttacca
    16861 cgttattatg aaagtgaaga ttatatttcg cataccgatg gaaaacgttc tttgtttgag
    16921 aaagtgtacc aaattgtaaa aggaattgca cttcggaaaa aactaaagct aatcaataca
    16981 atttcagaaa aagggaagct tctagatatt ggtgctggaa caggagattt tctatcagta
    17041 gccaaaaaga acaattggaa cgttattgga attgaaccca acaaaaaagc aaaatcaatt
    17101 gccacctcaa aaggagtttc tttggcaaac gatttaaaaa ctttggataa taactcattt
    17161 gatgtcatca ccatgtggca tgttttagag catgtgccaa atctagaaga gtatatttca
    17221 gaactgaaac gattaattaa acctacaggt accattatta ttgctgttcc aaattttaaa
    17281 tcttttgatg ccaattatta tcaagaatat tgggcggctt atgatgttcc aagacatcta
    17341 tggcattttt caaaaactgc catccaaaag ctttttgcaa agcaagaatt aaatttggtt
    17401 gatgttttac caatgaaatt tgatagttat tatgtgagtt tactttccga aaaatataaa
    17461 aatggaaaaa tgaatttcct caaagcatta cggataggat ggaagtctaa tcaatatgga
    17521 aaacggaaaa atgagttttc ttctcacatt tacattataa aaaataaata attataaaaa
    17581 acaaacatat aagtattaat tatagcaata tataaagcta taagatttta caataggtta
    17641 aataacgaac agaaaatgaa atttattttt tacttttgga atcttataaa tattaaatca
    17701 aaaatgaaaa aatcacttat cgttttagca atagttttta cttttattgc ttgtgaaaaa
    17761 acaactcaga ctaaagaatt taaaacggct tatattgata ccgctaagct tatggacgaa
    17821 agcatagagg gaaaagatgt agaagcaaaa tataaagaaa aagctaaagt tatgggcagc
    17881 caattggatg cagaagttgc acgttggaaa gacgaagctg ccaattttca aaaaaatgct
    17941 caagccaacg gacaagcttg ggcacaacaa aaaggtgctg aattgcaaaa aagagaacaa
    18001 caaatttcgt atgctcaaga cggaatgtta cgccaattgc aacaagaaag tagtgttgaa
    18061 atggattctt tagtgaaatc gtacaaaaaa atcatcaaag aatatggtaa agaaaaaggc
    18121 tttgattaca tttatggaac tggagaagca aactcagttt tatacgccaa ggaccaatat
    18181 gatattacca aagaaattat caaaatggtg aatgataaat acaaagcagc tgataaaaaa
    18241 gaggttaaag ctgatgataa atcggaagca aaaaaagctg ataaaaaata atattatacc
    18301 agtttttaca attaaaatgc cttttataac gaaaggcatt tttttattgt ttgttgaaat
    18361 acaaagtttt atatttgttt ttctttaata tacctgccaa atggaaacgc tatctgccga
    18421 agcaaattac gtcttgcaat ttatcaacca aaccaaccgt tcgattttcc taacaggaaa
    18481 agccggaaca ggaaaaacaa cacttctcaa agaaatcatc aattctacac acaaaaattg
    18541 tgtggtcgtt gcgccaactg gaattgcagc tttaaatgca ggcggcgtaa caattcattc
    18601 cttgtttcag ctaccttttg gtgggtttat tccagatagt tccgctaatg cacatttttc
    18661 aagccagata aagtttgaaa cgaaagctac actaaaccgt cattttaaaa tgagtgcttc
    18721 aaaaaaagca gtaatccaaa atatggaatt gctaattatt gacgaagtaa gtatgttgag
    18781 agcagatttg ttggatgcca ttgattatat gctccaaagt gttcgaagaa aaaaacaagc
    18841 ttttggtggt gttcaagttc tttttattgg tgatttatta caacttcctc ctataattcg
    18901 agaggaagaa tggaaaactt tacgcaacta ttattcagga aaattctttt ttcattcgca
    18961 tgtagttcaa actaatccgc cgttatatgt ggagctatct aaaatattca gacaaacgga
    19021 tgaaattttt attgatttgc ttaataactt aagaaacaac actataactt ctaagaatat
    19081 ttctttttta aatcaatttg tacaaccaaa ttttaatttg aaagccaata aaggatacat
    19141 cacattgacc acgcataacg ctaaagcaga tgctataaac gaacaatcgt taaaagattt
    19201 ggaaggcact tctgtaaagt ttaaagcaaa tatagttgac gattttccag ataaaatttt
    19261 ccctttagaa gaatatttag aacttaaagt tggagcacaa attatgttca tcaaaaatga
    19321 tttgtcattt gagaaaaatt attttaatgg aaaaatgggt tttataaaat cattatccaa
    19381 cgatgaaatt tgggttcatt ttcctgatga aaataaaaca attgaagtgg aacgctatga
    19441 atggcaaaat attaggtatt tcgtaaatga caataccaaa gaaattgaag aagaagtttt
    19501 aggaactttt gtgcattttc caatcaaatt agcttgggca attacggttc ataaaagcca
    19561 aggattaaca tttgacaaag cagcacttga tgtatcacaa gtttttcttc ccggacaggc
    19621 ctatgtagca ctttcgcgtt tacgttcgct aaatggcttg attctgttat ctccaattca
    19681 aatgaatggt atttcaaacg atgacgatgt gatgaattat tctgcaaata aagcatctac
    19741 tgatgtttta caaagtgaac tgcaaaaaca aaccaaatta tttcttcatc actatttaaa
    19801 aaacagtttt gattggaccg aacttgcaca agaatggcgc aatcataagt ttagctattt
    19861 agacagcgat ttatctgcaa aatcaaaaca caaaaattgg gcagatgaac aacttgattt
    19921 ttgtgaagct ttgcttgatc cagcaaaaaa atttaggtcg caattagata aaattttcaa
    19981 cgatgataaa gttgatttta aattcataaa tgaaagaatt caagcagctt atggttattt
    20041 ttttgaaaaa atagatactt tagaatatca aattttatat aaaatagaag aaattaagcg
    20101 cattaaacaa gtcaaacagt tttatcaaga gttacaggtt atagaagaac tgcaaactaa
    20161 tgctgtttta cgattaatga aagctaaaaa gctgattgaa aatgttatga acgaaaccat
    20221 tttggacaag caaagtttaa attcgtcgga aataaaaaat tataaaagaa ttaaaacaga
    20281 acgtgtcatt gaagatttta aaaagaaaaa catcaattta atcgaagaac aagaagacgt
    20341 aagttactat caacccaaaa aaaagaagtc tgaaaaagaa gcaaaaaaac caacgactca
    20401 aattacttat gaactttggg ttgataaaaa ttcgattgaa gaaatcgctt taattcgcaa
    20461 gttaacagtt ggaacaattt tgacacattt tacaaaacta gttcaagata aagccgttgc
    20521 aatcaatgat gttttacccg aagacaagct gaaaagttta tcaaaactat ttgagaatta
    20581 taatgaagaa actttaaata ctatgaaaga acaagttgga gaagattttt catggaatga
    20641 attacgattg tttaaagcaa gtttgaatgt ttaatttaac caataaaccg ctaaaaccga
    20701 aggtataaaa taaatagcaa tcgttcttgc accgtcataa tcttttgcca gcctttgacc
    20761 aaacagcatc aatattaaag taattgacga taaaacagca ccataaaaac caaaagtcct
    20821 tccgccatta attagtaatt gagcgcaacc aacaagcgtt aaaattccag tacaaagttc
    20881 aagaaacact aataaaacaa tagattgtgc aacataattt tttaaaatac ttgtttttga
    20941 aaaatgaact ttaagaaatt caacgtttgc tttccaatca aaaattttat catacgctga
    21001 ttgaatgaaa gttatagcca aaaaaactaa aattagtagc gaagcaggat tgttcatatt
    21061 tttatttttt atggattaga cgagttaatt ttatggataa gtcagtcaaa actatctttg
    21121 catttccatt ccgttcaatc atataaatgg catccgatag tgactcaaaa atctctgaaa
    21181 tgttgtttcc agtaacgaat ggtgcaaatt tatcaaaggt aaaaccagaa gtttgtggct
    21241 caacataaac caattccttg gtttcataat tgtgtaacaa tgcctgtcga aacattacga
    21301 tacaataatt taaaaactgt ttttgacttt cacgaccaat gcttgcaatc gaatcgctcc
    21361 aagcaattaa atcataaatt gccgtagcat ttcctttggc tttaaaagca gaacgaaccc
    21421 aatgtacaaa ccattcctca aacggtaatt cgtcttcatc atgtttcaaa aaatgcatcg
    21481 ctttattata atttccttga gattgatgtg caattttatc agctaaattt gtttcaactt
    21541 gaaaacgatt tactaaagat gctgcaatta ttttttcggg caatgcacca aaatgcaata
    21601 cttgacaacg agaacgaatg gtttgaataa tagcctcttc atcttcggca attaatagaa
    21661 aaagtgtttt ttcgggtggt tcttccagca attttaaaat cttattagaa gcggcaatgt
    21721 tcattttttc tgccatccaa acaatcatga ttttataacc accttcatag gattttaaag
    21781 caagcgcctt caaaatatct gtagcatctt ctactcgtat ttcgccttgt ttgtttttta
    21841 caccaagatg tttgtaccaa tcaaataaac taccataagg atttgcaagt gtaaattctc
    21901 taaaatcagc taaaaaatca gcacttttag gctttgattt tacttcatcg ttggaagtat
    21961 ttggaaaaat aaaatgtaga tcaggatgcg aaagcgattg gaatttcaga ttacaacttt
    22021 ggtttccttc tgaattgtct tgggaagtat ttttacacaa aaccaattga gcataagcaa
    22081 ttgccatagc caaagttcct gagccttctg gaccaacaaa tagttgtgca tgtggaattc
    22141 gacctgcaac ggcactttta atcaaatgat ttttaatgta atcttgtcct ataatttccg
    22201 taaaatgcat aaagcaaata taaatgaatt tagaattgaa aagagtaatt tgttctaaaa
    22261 aaagccattt tatttcatgt caaatggttc taaaaacaac acaaaaatcg atttttgccg
    22321 ataaaaatcg acttttatcg acaaaattaa gtataattgc agtgagttta agataaaaag
    22381 taacaggtta aaaacagaaa ctcactcttt tttagcaatt tatttaataa aattaaagaa
    22441 actatttata aattcttgaa aaactattac ttacaaatgt cttatagtca ataaattaat
    22501 gtaaataaag atggaaaaaa acaatcattt atctctttta atttttttac ttcttactat
    22561 tttttcacat cagttgtccg cccaattaaa cgtaccgttt aaagttagac accaatcttt
    22621 tgttaaaggc gacatgacaa ttattgctaa caatattgta aacaggaaag attcatttgt
    22681 caaagcagtt gctccctata acagcaccga ttatagcgca aaacataacg atgaattaaa
    22741 catggagtat attgatattg acaacgacaa ctccactttt tcatctagta atgctaattt
    22801 ttcgacggaa aaaacggcca atttaaaagt ggtttatgct ggtttgtatt ggtcagcaac
    22861 ctataagtac aatataggaa aacaaaaaag aaatcgaaaa tatgttgccg tagataaaga
    22921 cagagatgct tttgatgttg ttaaattgaa attgccaaga caagaaaaat acatagacat
    22981 aaaaggagaa attttatttg atggattgaa cgaaaatggc tttgaagaaa atgcgccata
    23041 tgctgtatat gccgatgtta caaaactgct tagcgatctt caaaatcctc aagggaatta
    23101 cacagttgcc aatgtaagag ctacaaatgg aactttaagc ggcggcgttt ctgctggttg
    23161 gacaatgata attgtgtatg aagacaagca agaaacgggt aaatttatta ctacctatga
    23221 tggttttacg ggtgttagca aagaatcatc cgatattgat tttaatggat ttcaaacctt
    23281 gcccgaagga aaagtaaatt tgaaaattgc aggcgcagga ttagaaggcg atttaggatt
    23341 aaaaggtgat caaatttcat tcaaatcagc cgaaaatgaa gattttacaa ttctaaatac
    23401 taaagttaga gattttaata attttttcaa tagttccatt actattaacg atgaagtgtt
    23461 tgctaaaaga tttcccaact ctcaaaatac acttggttat gattcttttt taatgacggt
    23521 ttataatcca gacaatcaag ttattggaaa caacactact gaagcaaaat taagattcaa
    23581 atcaacaggc gatcgctatt ttatgttttt tagtgctttt agcgttgaag ttttagagac
    23641 ggcaaaagct attgacgaaa ttaaaccaaa tgaactacta gttgaaaaca aaacacttcc
    23701 aacagcaaca aatacaactt ctttgtcaaa tgcagttgct ttgaatgaaa ctaccgaaaa
    23761 tacaattcaa aaagaagaag ttaaagaagt aacgcaatat aaaattactg ctacaaaaga
    23821 aaaagaggaa aaaagccaaa ctacaacaaa aacaaatgac aatgtagtgg caacaaaaac
    23881 agtaaaaaca acaactaaaa aagctgataa aataagagta acacctatag aatctaaaag
    23941 tagaagtttt cctggtgaag aaaaaggata ttatattatt gcgaatgtat ttgcaaaaca
    24001 ctcaaatgct actcgatttg tgtcaaaact tcagtctcta ggaattgaag cgggttattt
    24061 cataaaccaa gctaacaatt atagatatgt atatgttagt caccacgaaa atttttcgga
    24121 agcacaaaca ctttattttt caaatattaa tgaaaagtat tttggcgatt tgtggataat
    24181 gacggttaac aaagaaaatg acaagattgt taaaaataaa attgaaaata ctacttttgc
    24241 aaaagataaa gatgtggcat tgaccatcaa aaatactaca attaaaaagt taattaagag
    24301 tagcttaagt ttttttgagg aaagtctaaa ttatatttgc catttcaaac aaaaggaatc
    24361 ctaaacaaac caattgaaaa caactatcca tttttaatat gaaaaccatt aacgatttta
    24421 attttaaaaa taaaaaagcc atcattcgag tagattttaa cgttccttta gacgaaaagt
    24481 tcaatgttac ggatgtaaat cgaatagaag cagcaaaacc aactattgat aaaattttaa
    24541 tcgatggcgg aagtgttatc ttaatgtcac atttaggacg accaaaaggg aaagaagaaa
    24601 aatattcgtt aaaacatatc attgctactt gttcaaaagt acttggagta aatgtaaaat
    24661 ttgctgaaga ttgtcgtgga gcaattgcta aagaagcagc atcaaatttg caaaaaggtg
    24721 aagtattatt gttagaaaat cttcgttttt atccagaaga agaagctggc gatgttgaat
    24781 ttgcaaaaga attagcttcg ctaggtgata tttatgtaaa cgacgctttt ggaacagcac
    24841 atagagcaca tgcttcaacg acaattattg cccaattttt tccagaaaat aaatgttttg
    24901 gattattatt ggcaaaagaa atcgaaagtt taaacagagt gttaaataat tcggtaaaac
    24961 cagtaacagc agtacttggt ggttcaaaag tttcatcgaa aattacggtt atcgagaata
    25021 ttttagacaa aatagatcac atgattattg gtggcggaat gacatttacg tttgtaaaag
    25081 cattgggagg aaagatagga aattccattt gtgaagatga caaacttgat ttagccttag
    25141 agattctcca aaaagcaaaa gaaaaaaatg ttcaaataca tattccagta gatgttgtag
    25201 cagccgatgc tttttcaaac gatgccaata ctaaaattat tgatgtaaat caaattcctg
    25261 atggatggca aggtttagat gctggaccaa aatcgttaga aaattttaaa aaagtaattc
    25321 ttgacagcaa aaccattctt tggaacggac cattgggcgt ttttgaaatg gaaaattttg
    25381 ccaacggaac tattaaatta ggtgaattta ttgccgaatc cactaaaaat ggagcatttt
    25441 ctcttgtagg tggaggcgat tctgtagcag ctgtgaaaca atttggctta gaagataaga
    25501 tgagctatgt ttctactggc ggaggtgcta tgttagaaat gcttgaaggt actgttttac
    25561 ctggtattgc agctatttta gaataatttt ttaaaagtaa cacaataaat ttcaacaaat
    25621 tgcgttatta atagaataaa ataccacttt agacaataaa gttatgaata aattaaaaat
    25681 cacttcgtta gcgattgttt tgctaacaag tatcggattt gcacaagaca ccatcattac
    25741 agctcaggtt aagaaagaac cgttaaccag acaacaatac ttagattcta tcaagggaac
    25801 ttttgttcgc gataatattg catcttgtat tgatagtatg tggatgaaag aattagcaaa
    25861 tctcgattta tatgatgaat tgagtttaga tatacaaaac attgatgtag atcaaaaagt
    25921 tgattatgaa cttcctacgg aattattaaa agaacaatta aaaaagctta accaaaaatc
    25981 ggcttttaat atagaataca accaaggatt agaaaacgta atcaaatcgt ttttaaaaaa
    26041 cagaagaaaa tcatttgaac gattaatggg aatttctcaa tattatttcc caatgtttga
    26101 agaatcgttg gcagctcaaa atattccttt agaaataaaa tacttagccg ttgttgagtc
    26161 agccttgaat ccaagagccg tttctagagt tggtgcaacg ggtttgtggc aatttatgta
    26221 tcaaaccgga aaacaataca atctaaatat tgatacgttt gttgatgaaa gaagcgatcc
    26281 gttaaaagct agtaaagcag caacacaata tatgaaaaac atgtatgcta tttttggcga
    26341 ttgggatttg gttttagcat catataactc tggaccagga aatgttgcca aagccattcg
    26401 tcgttcgggt ggaaaacaaa attattggaa cattcgtccc tatttgcaca aagaaactca
    26461 aggatatgta ccagcttttt tagctacgat gtatattttt gaatatcaca aagaacacgg
    26521 aattgtgccc aacaaagcaa ttgccaacca ttttgcaacg gataccgtaa acataaaaag
    26581 taaaatgaca ttcaaacaaa tttcagattt gttggacgtt cctgttgcac aactacaatt
    26641 tttaaatcct tcatacaaaa gagatgttgt tccttttata actggagaaa atcattattt
    26701 acgattacca atagacaaaa ttgcgcgatt tgtttctaat gaagaaaaaa tttatgctta
    26761 tgtcaattat gaagattcgt taagagaaaa accatttgcc aacaatacag ctattgctgc
    26821 caatgatgga aactcaacat caagattaaa atatcataaa gtcaaacgag gcgatacttt
    26881 aagtgaactt tcggaatcgt atggcgtttc tatttctgaa ataaaaaaat ggaacaaact
    26941 tagaggtaat tcggtaccat tgggtagaaa attaaaaata tatacggaag aagctgttgc
    27001 ggctgttgaa aaaaaatcat ctaaaattga cacaacttct gtaaaagaaa ccaaatcagc
    27061 aattgcttca aacagtaaaa attatacaga agaaaaagta gtttcgttta aagatgtagt
    27121 gaagtatcac aaagtaaaac gaggcgacaa tcttggagaa attgctagta aatatgatgt
    27181 ttcggttgcc gaagtaaaaa aatggaataa gttaaaaaac aacaatatta atcttggtgc
    27241 aaatttaaaa atcatcaaaa atgaaagagt tgtaacggtt gttaaaaaag aaattaaacc
    27301 agaaaaaatc aatacaacta tagaagaaga aactcaagta gcttctaatg atgaacaaga
    27361 aaacccagaa gatttttatg tagttcaaaa aggcgataac ttatttacaa ttgccaaaaa
    27421 attcaatgtt tcattagaag atttaaaaga gtggaacaat attgaaaacg gaactattca
    27481 atcgggttca aagttggtat taaacaacgc ttcaacggct caaaacgaat cagttgaaac
    27541 accaaaaaca gaaacaaaga ttgtagagta tgtggttaaa agcggcgata atcttggtac
    27601 tattgccaaa aaatacaacg cttcaatttc tgatataaaa gattggaatg aaatggagga
    27661 tgataatatt tcagtaggtt caaaattaat agtttctaaa aaattattta tcgttccaac
    27721 tcaaatagct aaaacaagca aaacctctaa aaaccaaacc attgcacaat caaaaagcga
    27781 agaagtaaaa cactatcttg ttaaaaaagg cgattcgtta tttagcattg ccaaaaaata
    27841 tccaggagta acaatttcgg atattaaaaa gtggaatggt ataggaaacg aaagtatcaa
    27901 accaggcatg aaattaagaa ttaacggtta atccgaaaat cttcaatatc tttgggtttt
    27961 atttattaaa atgaataaaa cccttttttt atacttgtgt tttgcctttt tgaccttttc
    28021 gtgtcaaaaa aaagtcgatt ctctaagtca agaaaaagcg gcagcaatta atactatttc
    28081 tgttattatt gacgaacagc tttggaatgg cgaaattggc gatagcattc gaaataaatt
    28141 tgcttcacca gttttaggac taccacaaga agaaccactt tttaccataa aacaatttcc
    28201 gctaaaactt tttgaaggat tttctaccgg aagtcgcaac atgattattg taaaaaaaga
    28261 aaataaaagc gaattcaaaa ttggaataaa cgagttttct acaccacaaa attcgtttca
    28321 tatttcagga aaaaatacgg ttcaaattat agcattgctt gaagaacatt cggcagaaat
    28381 gattaaaaga atgcggcaaa ctgaaattga agcctgccta aaaacaataa aagattcgct
    28441 gctaaataca aaaacaataa aatcaaaatt tgatattgat ataacaattc caggaaatta
    28501 tgaatacgcg ttaaccagta gaaattttgt ttggataaaa aaagaaatta catcgggcaa
    28561 cattagttta ctgatttatc aagttcccta tcaaagaatt cacaagtatc atacggtaaa
    28621 tgacattgta aaagttagag attcaatagg aaaaaaatac attcacggca gaccaagaaa
    28681 ttctatgatg attaccgaag aagcttattt tccctatttt tctagaataa aattcaataa
    28741 ttttgatgct tacgaaacca aaggaacatg gcaaatgaaa aacgatttta tgtccggacc
    28801 atttatcaat tatgccctaa gagataaagc caacaacaga gttttgattt tagaaggttt
    28861 ttgttatgcg ccgtcaaaaa ccaaacgtga tttaatgttt gaagtagaat ccattatcaa
    28921 atcggtaaaa attctaaaaa aataatattt atggcaaccg aatggaaaat taagcgattt
    28981 caagcacttt ctttggaaga actataccaa atactcaaac ttagaagtga agtgtttgtg
    29041 gtagagcaaa attgcgttta tcttgacatt gatgacaaag atcaaaaggc attacatgtt
    29101 tttggacaaa ttgatgaaaa aatggtggct tatgcacgac tttttaatcg aaacgattat
    29161 tttgaaaacg cttctattgg tagagtggta acacatcctg attttagagc caacaaaatt
    29221 ggacatcaat taatgaaaca agccattgag gctattgaca atcattttgg agaaaaacaa
    29281 attaccattt cagctcaatt atacttaaaa aaattctacg aaagccacgg atttgaacaa
    29341 accagcgaga tgtatcttga agatgatatt ccgcatattg agatgaaacg aaattaaatc
    29401 ttcgtaatca ccaactcaac tctacggtca tattctgtgc cttttcccaa aggaacggtg
    29461 ttgccataac ctttaaacgt cattcgattt ttggctattt ttttgaaaac taaatattta
    29521 taaaccgatt ctgctctatt ggtcgataac tctctttttc gggtttcgcg gtcaatggct
    29581 tctttttgat actgaggcgt acagcaaaca tgtccttgta cttcaaattg caaattcttg
    29641 tacttcaata aaagtttggc aattttatcc aaagctgttt ttgatttata atccaacttg
    29701 ctacttccac ggtcaaaaag aatattgtct aaataaatat ggtcgccaac cacatgattt
    29761 ttttgaatag tattataaaa acccggaatt acaatttttg gcaatggttt taggttgata
    29821 accacatcaa cccgacgatt tttagagcgt ttttctggaa gattttctac aacatcgtca
    29881 tcaattaaga ttctgccttt tccttcgatt gtaacaataa ttttgttttt tataccgcta
    29941 tcaatcattt tttgctgaat cattgaagca cgcttagtag aaagtttaaa attataagct
    30001 tcttttccaa tatcatctgt gtaaccaaaa atctcaatgg cttcaattcg tgtggaatca
    30061 atcgttttta taaatttaat taaatcctcg ccttgttttg catttaaatc cgatttgtca
    30121 aagtcaaaat aaacagaaaa aacagcttct tcttgcgcat gaacaacaga agttaatagg
    30181 aaaaaagcaa ataaatagaa cagtcgcatt attgtagaat tttagaaaac gcagatttat
    30241 cgtttaacaa agtaaccgat tttttaactt cagcgttgtt tttaatataa tagtgataca
    30301 aaccttcttt gtactggtag cgtttgatga tttcgtctaa aatcaaattt ttaatttctt
    30361 tagcattttt gtcaatcatt gtttcttcac ttttttgaac agcagtcaat aattgttgat
    30421 attccgaggc aatagccgaa tctacattgt cttttttggc tttttccaac atggctttca
    30481 atgcaatttc agtatcagta ttgatattga ttttttcttt tttgataaaa tttttaaaat
    30541 ctgtcaaatc agcgtcagta atcgttggta ttttatcgcc aagtgttggg tttttataat
    30601 aataattggt tacataatta aaaatagcat catttttttc taaagcttca gcaatagtgc
    30661 tggttttaga ttcttccata acaacatctg gcaaaattcc accaccatca taaacggttc
    30721 ttccattttt ggttttgaaa gcggtataat ttttttcatc tgttctaatg gcttttccat
    30781 ttttatcttt tttggcataa tccaatgctt gaatgcatct tccagatggc gtataatatt
    30841 tagaaatagt aacttttact tgcgtcccat aggtcatgtc aaatggtcgc tgtaccaatc
    30901 ctttcccaaa acttctgctg ccaataatca cggctctgtc taaatcttgc aaagcacccg
    30961 aaacaatttc ggaagcagaa gcactttttc catcaacaat aatagcaatt ggaatttcgg
    31021 tatctattgg ttcgcgagta gttttataag tgttgttgta tttttcattt ttagactttg
    31081 tagtaacaat aatttctcct ttgggcacaa ataaattaca aatattcacg gcttcgttta
    31141 ataatccacc aggatttcca cgtaaatcta atacaatttg agtacagcca tcatttttca
    31201 atttttccaa tgcttctttg gtttcataag atgctttttt attgaattga gacaatacaa
    31261 tgtaaccagt tttctcatct atttttccaa aaaaaggtac tgcttttatt tctacttcat
    31321 ccaaaatcaa ctgtgcggtc attgcttttc cttgacgtaa gtattttaaa tctattttag
    31381 tatttttggc acctttcatc aattgcgatg catcgtcttt gaaatcagca attaaaacat
    31441 caccaatttg tatgatttca tcaccagctt taagtcctgc tttgtcggca ggaaaacctt
    31501 tataaggttc gcgaataatc agtttgtctt cttttcgcgt aatcatggcg ccaattccgg
    31561 tgtattcgcc cgtgttgtta attttgaatt taataacgtc ttgttcgttg aaaaaattgg
    31621 tgtagggatc tacttcaacc aacatgcttt tgatggcttt atccattaat tccgctggat
    31681 ttaaatcgtc aacataattt tgattaactg ttttataaac gttggtaaat atttcgattt
    31741 gtttggcaat ttcaaaaaaa tcttgcttga agcttacgcc aataaataga aaaccagtta
    31801 gcatggcggg aagtatatat ctttttttaa tttttggaaa cataattaat tggttgattt
    31861 atttttaagg caatacgaag atactaattc tcgtttttag tttcgttttc ctggagtgaa
    31921 ttaacaaatt tttcaaataa ttgaatggtt ttttgattaa tttcggcata ttctaatcgg
    31981 tcttttgatt gatagaaaaa cataaagcaa tatttttgat ttaaattatc aatcaacagt
    32041 tgtttgttta gtcgataaca ctctctcagc acacgtttga aatagtttct atcaaccgca
    32101 tttttgaaat attttttgga aaccgaaaca cccattttta gcggaatatc ttcttcaaaa
    32161 tctttttgaa tatacaccaa tcgcagcgga tatttaccta ctgattttcc ttcggaaaag
    32221 agtaaatcaa tgattttttt actttttaat ttctccgctt ttggataagt ataatccatt
    32281 attatttgac attgtaaatg ttgtaaacaa attttacttc attataattg atgtaaaatt
    32341 gcatgcgatt gccttctttc tttttggtaa taatcagaat tgtacattta gaaaacttat
    32401 tgtcaataca ttcaaacgga acaatattta cttcatcatt atcagtaatt tctccgtaag
    32461 ctttaatcat aaaggattgt acttcgttag aatttacggt gattaaattt ttctttgcat
    32521 ccaaggtaat caatacattg gcatcaacaa attcggacca atcgttccat tttcctttac
    32581 tgtctttgtc ggtaaaactt gcgctactgg ttttaaaacg aatgggttga gcacttacat
    32641 tttgaaaacc aaaactaaat gctagaaaaa ggatagtgat ttttaaaaaa ttcatagtta
    32701 aaaatatttg tggtaaatct acaaattcta ttccaaaaaa aaaccgttct aaaaaaggaa
    32761 cggttttaaa gttttatttt gcttcgtaaa cgttattctc acgtttgaca tctgccatca
    32821 aaccactagc atcaagtgtg atttttttaa tagctgattt tcctttagga atttcaaaga
    32881 aataattagg atttccccaa ttccatgaag gtaaagtggt tcgtttgatt gatggtgttg
    32941 gattttcttt ttcaaaaaat agcatttcta atggaacgta aaaactttct ttggaaccat
    33001 caatatattc taccataaca tcgattggca ttggcgttct accaattctt tcaagtgaaa
    33061 cacctgttct ttgaacacca tcagttgcag cttcaacatt ttttattcca taatcaatag
    33121 tgtttaaagt ttcagtccaa tcgattaaat accaacctaa atgtgcaccc gaaactcgtt
    33181 cagctgttcg tttaatatca ttcggcgttg gatgtttgaa tttgaaatcg gaataatatt
    33241 ttttaatcgt ttttctaagg ttttcttttc caattaaata ttccaattgt gccaaaaaaa
    33301 cttcaccttt actataggaa gcaatgctgt aacttctgtt ttcatcatat cggtcagcat
    33361 gggttgaaag cggttgttct ttgccagaat tggctaaatt gatataactt ttataagcac
    33421 cagcaaaagg attttctact tttttagatg ataattcgtt cattacccaa tcttcaatat
    33481 aagaagtaaa accttcgtcc atccaactgt gtttgctttc attggttgcc aaaacgtgtt
    33541 gaaaccacga atgtcccatt tcatgagcaa ttaaaccaac aaaaccgcct aaagttcctt
    33601 cgccaagcac caaagtacac atggcatatt ccataccacc atctcctcct tgtataacag
    33661 aatattgttt gtagggatat ttgccaacgt tttgattgta aaaatccatt atttttgaag
    33721 ttaatggttc agcttgtttc caaacttcgc taatttttgg attgtttttg tataaaaaat
    33781 gcagcgttac accatcagga cgaactaatt tgtcgtgaat ataatctttg tcggcagccc
    33841 aagtaaaatc atgaacgttt ggtgcgataa aatgccaagt taaggtttta gcatttttat
    33901 ctttcttaac tgatttgcca acatcttcgt aaccacaacc aatttcattg ttgtttttca
    33961 aataacccgt tccgcccaaa atataatttt tatcaatggt tatttttaca tcaaaatttc
    34021 cccaaacacc gtgaaactct ctaccaatat aggcatcagc atgccagcct tcgttgtcaa
    34081 attctgccat ttttggatac cattgcgcca tggataattc aattccttcc gaattatttc
    34141 ttccagaacg acgaatttga acaggaactt gtccgtcaaa atctaatgta aaacaggttg
    34201 attttccagg taataaaggt ttgttgagtg ttacttctaa gatagttcct ttttcaattg
    34261 cttttgcggc aactttatct tgcttgaaat tggatatttt caaatagcca atttcatttg
    34321 gttgtaattt ggctattcta ctttctttaa cttctttccc gtcaacattt ttaactttat
    34381 ctaccattct tccatcagga tcaacgatgt tttgcaatct catgtccatt tcacttcctg
    34441 gttgaaaagc gttgttatac aaatgataat atacttttcg aagagtgtct tccgagttgt
    34501 ttttatagac taatttttga gttcctttgt attgatagtt gtttatgttc atcgaaactt
    34561 ccatcgtata atctactttt tgttgccaat aatgtttggt ttgagaaaaa caaaaaatgg
    34621 gtagtaagag taataaataa aaatacttca taaaaaataa ttatgctttt ggattggatt
    34681 ttggttcttt tgaaagttgt tcggccattt tcatagcgtt ataagcattg acaatttttc
    34741 ccgaaacaca tgtttctgaa aaaggaatta atttgtggtc atcaccaact tcaacatcaa
    34801 aatttattgg cgttcccgat tgcatgatga tgtttttaat ttgtgaggct tttaaatttg
    34861 gataaaaaga acgcaacatc gcagccacac cagctacatt tggagaagcc attgaagttc
    34921 cttgttcgta tttgtatttt ccttcgggaa tagtggcata aattttcaca ccaggagcaa
    34981 aaacatctac atttgtttta ccataattag aaaaatcggc taccattttt gaaccataat
    35041 tgtagttaat ggcaccaatt tctataacat ttgaagcaat ttcggttttt ttatctttcg
    35101 tatcagcagg aaaacttggt tcggtatcta tattttttcc gtcatttcct gctgcaaaaa
    35161 caattaatac atctttgctt tcggcatatt ttatagcatc ccaaacccat tctttatgcg
    35221 gtgaaaaatc ttttccaaaa cttccgttaa tgacttttgc accattatcg gtagcatatc
    35281 gtattgctaa ggcgatgtct ttatcatatt catcaccatt tggcacggca cgaattggca
    35341 taatttcaac attttgggca acaccatcac cgccaatacc gttgccacga atttgagcaa
    35401 tgattccggc aacgtgtgta ccgtgaagcg aatcttttcg gtctttagaa tagacaacat
    35461 tgtttccata ttttttatcg ttgatgtcat ttggattatc accaacaatt tttcttccat
    35521 caaattcttt gtttaagtta aaattcaatt ggtcgtaaac atagtctttg aattctttta
    35581 aatcttggtg aaaatttttg ccttcacttt gttctataaa agattgcata atacgcttac
    35641 tttgaaataa ttcgtatttc accgatggca tattgttcaa ttcttctgat gtataatcct
    35701 cttttttaag tgcttctcga acgtttttat cagcatttaa gaacaaatca acggtttctt
    35761 ttaaaggaaa aactttgttg tatttttttt catattctgc taatgctaat ttgtattctt
    35821 ccgaaccgtc gtttggtttt tttaacattc taaccaattc taggttttca ttattggctt
    35881 cgcctaaaaa gttccatcca tgaatatcgt caacatatcc gtttttgtcg tcgtcttttt
    35941 tgttgttgac tttttcgttt ttattagtcc aaatagtaga ttttaaatcg ggatgatcaa
    36001 tgtcaacacc cgaatcgatt acagcaacga ttacttttga gccttttttg tctttgataa
    36061 gttcagaata tactttatca acactcattc caggaatcga atctttctca agatcaagat
    36121 gactccaacg ttgtaactgt agttcgttaa gtgactgttt tttataattt tctgaaattg
    36181 aaatttgaga aaatgctgaa taagataaag caattaggta aattgctatg aactttttaa
    36241 tcatgatttt ataatttagt atcaataaac tagtgcaata ataattcctt ttgttacact
    36301 taataaaagg attaacacta tcttaagatt tcatcacaat gaaaaacttc tttcaaacga
    36361 acaccttttt cagtattttc aacggtaata atttcgttat gagcatcgtg ttctaaccat
    36421 aaatagaaat tattttcagc agcagttttc aaaaattttt ctttttcggg catggttaaa
    36481 agtggtcgtg tgtcataacc catcacatag ggtaaaggca aatgtccagc agttggcaat
    36541 aaatcagcac aaaaaacgat ggttttatca ttgtatttaa tatgtggaat catcattttt
    36601 tcggtatgtc catcaacgta gtaaattcca aaattcattt catctgaaaa gccaaaatcg
    36661 gaatctggtt tttcaataaa atgaagttgg ccactttctt gcattggaat tatattttca
    36721 tgtaaaaacg aagctttttc acgattatta ggttttgttg cccattgcca atgattttcg
    36781 tttgtccaat atttagcatt tttaaaagca acttcgtaac ccgttttgtc cttattccaa
    36841 ttgacacttc cgccacaatg gtcaaaatgc aaatgtgaca taaaaacatc ggtaatatca
    36901 tcacgatgaa aacctatttt ttgcaacgaa ctatctaaag aatgatttcc ccaaagtgaa
    36961 taatagctga aaaatttctc tgattgtttg tttcccatac ctgtatcaat taaaattaat
    37021 cgttttccgt cttcaatgag catgcatctt gcagctatgt cgatgaggtt gttttcatcg
    37081 gcaggattgg ttcggttcca aagtgttttt ggtactacgc caaacatggc accaccgtca
    37141 agtttaaaat ttccggcttc tattgggtat attttcatgt gatttttaat ttatacaaag
    37201 ataacattgt ttatttaatt tgaattgaag ctttaaaagt gaagttttag tatttttata
    37261 aaaatttgct ttgatgttta cttttaatac aattttacac aaattaagat acttaaattg
    37321 aaaacaacca taatcattat gaataagatt attttttact ttttaatagc cttctacagc
    37381 actacatttt acagccaaaa caatcttgaa gtcttattaa aaaaagttga ttcattagaa
    37441 aaaagcggaa attataaaga agtacagctc gtctttgaac gtttaaaaaa agaaaataaa
    37501 aataaaaatc agttgtatta ctcagcaaaa tttttatttg caaaaggtat gtctatggcc
    37561 aataatggta actacgatgc agctaaagaa aatttcagca aagcgataca agtagcaaaa
    37621 aaaataaaac caagccacaa ttcaaaacaa ttattaagta gaatatattt agaattaagt
    37681 tattgttatt ttgactggga agtagcattg aaacaaaaca ctatcggata taattatacg
    37741 ttaaaaaatt taccaaaaga cacactatta attgattatt tgtctgatcg tggattcatt
    37801 cataagcaat taaggaatta cgaacaatcg attttggctt ctgaaaaagc attgaaatta
    37861 atcgctaaat tacaaccaaa aaagtatgat ctacttggcg atgttcacaa taatcttggt
    37921 attgcttatt ctgatttgca tttgcataat caatctttgt atcattatgc aaaaggttta
    37981 gaatataaca ttaaatcaaa ccaaaaggat aaatctgata tagtcaactc tgcaaataat
    38041 accatttggg aaagtttgga ttatggtgat gaaaaaaagg caaaagaaat tttaaatttt
    38101 ttaaataaaa acttttataa gtggtataat aataaagatt ttgcaacgac agatgtgtct
    38161 gcgaatttgc aatcttgggg gctttatttc aaatcaattt atcatttgtc aaatttaagg
    38221 aatgaaattt atgataaaaa ttattttgaa gcaaaaaaac actttgacac catcgtttca
    38281 atatttgaga aatacccttc agagcgaaag aagaaagaca atactttact gcttagccga
    38341 tatgaatatg aagaaatttt taaatcaaaa cctttaacgg atgaactaaa catcaaacag
    38401 catatcgatt ttaataaaag aacaatacaa atagctagaa gagacgaatc taagcacgaa
    38461 gaattggtgg cgtgtttaaa attagctata gcttttaata gatacaaaaa atatgatgat
    38521 gcacttaaaa ccattgatga agcaaaaaaa atagacgaaa cattttttaa tgcatcacga
    38581 tttactattg gcgttttaga agccacagta ttaaattctt tgaacaaaaa tgaagaatct
    38641 cgaagagtta ttttaaaatc gtatcaaaaa ttgttagaaa aacaaaaaaa aattaaaagt
    38701 ttacaacact taaaatatgc ggattttaaa aaatttaaca gcagtacttt tattagaaat
    38761 accattaatt cggcaacaat ttattttaaa atctatcaga aagataaaaa taaaggagac
    38821 ttattgattg ccaacaattt attttttata gcttctgata tttttgccga attctaccaa
    38881 aaagggaaat ataattacca tttaaatgct ttcaataaag aaattgcttc gggactttta
    38941 gaaacgcaaa tgctaatcga cccaaatgat aaagtaaaaa taaaagcaat actcaataga
    39001 attgaaaata actcatcgca gcatttgtgg aatctttttg aattgaaaaa cagtcaaaat
    39061 ttgaaaatac ctgcttcttt aatccgaaaa ttaaatgaac ttgtttttga aaaaaacagt
    39121 ttacaagagc aacttgaaaa aagcaaatct tctgttgaat taaaaaatca actagcaact
    39181 ctcaataaag aaattgataa aacacaaaaa gcaattaatc aaaaggatgc atcttttcaa
    39241 aaattcaaat catctgattt tgatgttgac aacgtccaaa aacaactttc ttcaaatcaa
    39301 attgtagtta agtatcttgt aatggataaa aaaacattcg catttaccat ttcagataaa
    39361 agcattcaat tagttaattt aggcgatact gaattattaa aatcaacggt tttacagttt
    39421 acgaaatcaa tcaattcgat tgataataat tatactaaaa tctcccaaaa tttgtacggc
    39481 aaactaatag aaccaattct caaagaaaaa caagttgcta ccatcatttt tgtacccgaa
    39541 gattttttga atgctatttc ttttgaaagt ctaatgaata aaaacggtaa gtttatagtg
    39601 caaaattata ttgtaagtta tgcttattca ttaaaaatat gggatttttt acaacaaaag
    39661 caaacagcaa ataaagatac ccatcaattt gtttcatttg ctcctaatta taacaaaaca
    39721 cctaattcaa accaaaatag aggattacga agaggtaatt tgttcgattt ggcagaagca
    39781 aaaattgaag cacaaaccat aaacaatatg tttaacggaa ggttgttttt aaacgaaaag
    39841 gcaactaaaa ccaacttttt acaatcaacg acacaatttg attttcatca tttggcgatg
    39901 cattctatgc ttgaagatga ttattccaat tcatctttag ttttcacaaa taaccaaaaa
    39961 gtgtattttg atgaattgta tcaacttaat tttccttcca aaatggtcgt tctaagtgcc
    40021 tgtagcactg ggcttggcac gcaagaaagc ggagaaggca ttatgagttt atcaagagca
    40081 ttagcctact caggtgtaaa atcctcagta tatagtcttt ggcaagttcc cgataaagaa
    40141 acttcagaaa tcatgatttc gttttatcaa aatctcaaaa aaggacaacc aaaagacgaa
    40201 gcattagcca acgccaaaac tacttttatt tccaataatc cgatgaaaaa acatccattt
    40261 tattgggcag gatttgtagt taatggtgat gtttctccaa tcgtttccaa ttcaaattgg
    40321 ataatttata taggaatagg cttagtcatt ttagcattaa ttttcttttt tagaaagaaa
    40381 ttattccaat tcagacaata actttttagc catttcttgt tgtggattat cggtttcagt
    40441 caacgatttc aataacggaa tggctttttc tttttgattt tcttttacat aagaaagtgc
    40501 taaataccat tttacaaaag gtgtaaaagc attatttttg gataagtcaa attgtttaaa
    40561 agtcacaatt gcatctttag ttttttctaa ttccattaat gataatcctt tataaaacaa
    40621 ggcataatca acattttctg aatcataaat tttagaaaac aattccaaag attttttgta
    40681 attcccatta tcatattcaa aaaatgcttc tgatttacta tcttctttca tttcacctcg
    40741 aacggttggc gcaacaacat ttggatagga ttgataataa tcgtcataaa ttgaagaagt
    40801 tgcgttttgt gtgaaataaa atcctacacc aaaaagcaat ataatggaag ctgctgcata
    40861 ccaaatttta aacgatttta cttttggttt tgaagtttca aaagattgta atttcttttt
    40921 caaatcactt ctttcgttta aagtaattgc tttttttaca cctttttgat aggatacttc
    40981 tttggcaaaa tcgacattgg tttccatcaa tagcacaaat tgcttttgct cttcttccga
    41041 aagtgatttt tcaaaatatt tattgattaa tgtttcgttg tccatttttt ataagtttac
    41101 tagttctgaa acaagtttag atgttcagtg atttttattt tttgattaag tctttcaatt
    41161 gttttaaaca acgtgatttt tgacttttta aaacgtcttt attggtatat tctaattcaa
    41221 tttgaattgc atcgagtttt ttttcttcat aatagaacaa ccgtaaaact ttttgacatt
    41281 tttccccaag ttttttaaaa ttatctcgaa gtatttttat ttcttcttcg tcttgctctt
    41341 cttcttcatc ttcaaccact tgaggcaagt gatgatcgta atctattgaa atattgatgt
    41401 tttttgactt caattttttg taaatcatgt atttgccaat tgaaaataaa taagttgtta
    41461 tgctgctttc taaactgtct atcgttcctt ttttagcatt ttcacacaaa gcaacaatac
    41521 tatcttgata aacatctaat aaatcatctg atgataaatt atatctgttt gcaaataaca
    41581 aaaacccatt tttattgtca acatatatac tatgaatagt tttttcatcg ccttttttta
    41641 gctgttcaag tagaatcaaa tgatttggtt tactcattag tgtacagttt ttctcaaaag
    41701 taaacataat tacttcaaaa aataaatttc aaaaatagat gtttactttt ttttatttct
    41761 aacactaaat tggaaaatga atctaaaaat taattaataa atagaaatta tgaaaacaat
    41821 tttgaccatt gtaattacac tgtttactgt atgtatgtct ttttcacaag aaacagtagg
    41881 cgaaagagtt gtaaaaaatg ctaaagacaa aacctatcaa cgtggtgaac aaaaaagtga
    41941 tgaaacggta gatggtgctt taaacaaagt tgaagaaggg ataaagggga tttttaaaaa
    42001 gaaagacaaa aagaaaaagc aagaatcagc agcagaaaat caggaaacta aagattctgg
    42061 aattcaaaat agttcatctg aaaattcttc aacaaaatca ataaagaaaa attccaaatt
    42121 tgattttgaa ccaggacaaa aacaattggg tttagatgac ttctcaacta ccaatgtggg
    42181 tgattttcct ctaggttgga atacgaattc ctctgctgaa attgtaacat tcgatgaaaa
    42241 caatcaaaag tggctttcta tcactaaaga cggttttttt cttccagaat ttattaaaga
    42301 tttaccagaa aattgcacta ttgagtttga tgtttttaca agatatgttt caaataacat
    42361 tttgaattac tccttttaca tcaatgcggt tgaaaattct aaaaaggatt tagataacaa
    42421 atacatccaa aatggattca actttaattg gcgaccagtt gttggttcag ctagttattt
    42481 tgtctatgaa aatggagaag aagtgagtaa aaatgaaggt ttaaaagtaa atgctttttt
    42541 aaccaataat ccagaaaatc caattatagc aagagtttct atttggcgtc aaaaatctag
    42601 attacgtgta tatgtaaatg aagataaaat tttagactta ccacaagctt ttgacccaaa
    42661 attaaaatac aactcgttca agtttggcgc agaatacatg aactattcta acggaagaga
    42721 acaagacgat gagtttatgg ttgcaaatgt tcggtatgca gttgcaggag aagatacgcg
    42781 aagcaagtta attactgaag gaagatttgt aactaacgga attttgtttg atgtgaatag
    42841 tgattctata aagccagaaa gtggtacggt tttaaaagaa attgcatcaa tacttcagga
    42901 aaatccaacc gtgaaagtga aaataattgg tcataccgat agcgatggcg atgcaaattc
    42961 aaatctcgtt ttatctaaaa aaagagcaat tgctgtaaaa acagccttat caacttttta
    43021 tggaattgat ggttctcgaa tagaaaccga tggaaaaggt gaaagccaac ctttaaataa
    43081 aaacgcgaat gctacagaaa aagcaactaa tagaagagta gaatttatca aactttaaaa
    43141 acattaaata atagaaaaca tgtataaaaa aataatttta atttttatcc taagtgtttt
    43201 aggaaaagca aatgcacaat cttttacaga cggtattttc accacaacgt ttggaaacat
    43261 ttcatggaca acagaaaccg gtactgatta tccaaacggc ggaatggtgt atggcgatta
    43321 taaagacaaa ggaacaatta tagggagttt tgataataat ggaaaagagg ttgttgggtc
    43381 atttcataac ggtgcagccg aaggtaaatt tgtttttata actccttatg gaaaggataa
    43441 tttttttgca aatggtatca aaacttttgc tggaaattgg ggttatagct caaacaataa
    43501 atattctcaa aatcctgatt atgaatggaa agttacaggt aaaataggcg agaaagatgc
    43561 tgtaaaaaat gttaccaatg tttggagtgg aaaatggaac actacggatg gcgcaatgta
    43621 tttaatgcaa gttggaaaca aaataacagg aacatacaaa ggtgttggaa ccgttgaagc
    43681 tacttataat ccttcaacta gattgttaaa aggaactttt acaaacaaca attttaataa
    43741 aacaggttac atcgaatttt attttgaagg caatacgttc aaaggaaaat ggggttggaa
    43801 tacaagtatg actgaaggca attgggatgg aacgaaacat gttaaaaaca ataaagagtt
    43861 atcgaagaac gtcttaacta atcagctaag caattcatct tctaaaaaaa tattatttag
    43921 aattcaagct aagagtattt atttgaatga acatggagtt aatccttcaa atgttagtct
    43981 atatggattt gttggtttcg agctctataa agtaactaat aattcaaggg ttgaggtctt
    44041 aagttttgga aataaatcta aatatttatt caatactaac gaaaatcaag cttttgtaga
    44101 aggatcgact tatatttttc caaaccaacc tacgtattat agagaatatg aaatttctga
    44161 agctgattgg aataataaca atattcaatt tgagcttaaa gtatttcatc atataaaggc
    44221 tgaaaaattt ggatcaaata gagatggtaa atatttttca gaaactttag attttaaatt
    44281 aataaaactc aatacaaatc tagtagctaa aagagaatat caaaaagagt ttggagaata
    44341 ttatggttta ttaaattata taattactaa attataatta ttatgaaaaa actactattc
    44401 actttaacaa tcctcttact tttctcatgt aacaaagcta aagaaagtgc acaagacgca
    44461 attacaggta caatagaaga aaccattgaa aacaaaactg gaacacaagt tgaattgcca
    44521 gacgccgatg aaatggataa taatgctgca tatattacct ataaatcgga aagcaaaaat
    44581 tatctagatg gaaaagaaaa aatgcaagca tcggttattt ttcaaaaaga taatgacggt
    44641 ttatctattg ccttgcaagg tgttggcgaa ggtggaaaat cttttgttac aactctaagt
    44701 catgttcccg aaaattttaa gttgccatta aaaggaaaat ttgctgtaaa caatcgttat
    44761 gatggtaaaa atgcttccgc agtaattatg tttatgaatg ttactgataa tggtatgatg
    44821 acttcagaac ttccgtatga aggcgaaatt tccattacaa aactctcgaa agatagtatt
    44881 gaatttgaaa ttaatggaaa aggcggcgat gcgtccgatg ctgaaagtcc aagtaattgg
    44941 aaagccattt ctggtagtgg aaaaatgatt tcacctataa ttatgagcta cggaattgat
    45001 aaaaacaacg ttttaaaata aattacaatg aaaacaaaac tcacaataat tatattttta
    45061 attcaactgg cattgttttc tcaaaacaat actgtttcat cgggcgcaac agcaactggc
    45121 gcaactggaa gcgcaactta cactattggt caaataggtt atacaacaaa tacaggttca
    45181 aacggaacta tttctcaagg cgttcaacaa gcttttgaga ttgtaacatt aagtacaaat
    45241 gatgttcctc aaattcagtt aacagtaata atttatccaa atccaacagt tcaaaatgtt
    45301 actttattta taaaagattt cgaattaaca aatttgaaat ttcaactttt tgacatacag
    45361 ggaagaataa tttcaaacgg taaaattaca caaaatgaaa ccgagattga aatgacttca
    45421 ttagcatctg ctcaatactt tttaaaaata tctgatagta atagcgaaat taaaacattt
    45481 aaaatcatta aaaaataaac atcatgaaat taactataaa aatatttttt cttttttctt
    45541 gcttcttatc tctattttct gcatttgctc aagcaccaga aaaaatgagc tatcaagcta
    45601 taattcgcaa tgcttcaaat acattaatca ctaatcaagc tgttggaatg cgagtaagca
    45661 ttttgcaagg ttctgctaca ggaacagaag tttataaaga attatttaat ccaaatcctc
    45721 aaactaatat taatggatta gtaactgtag aaattggttc aggaattcca ttaacaggaa
    45781 catttgctgg aattgattgg gcaaatggac catttttcat caaaaccgaa accgatccaa
    45841 caggaggaac ctcttataca attgttggaa caagccaatt aatgagcgtt ccttatgctt
    45901 tatatgcaaa aaatagcgga aatgctagct gggaattaaa tggaaacaca gcttctgcaa
    45961 ctaattttat tggaactact aatgattttg atttattttt taaaaggaat aatgtgaatg
    46021 ctggaaggat ttcggaaaat aatacatcaa ttggttttaa cgcgcttaat cctattagtt
    46081 ctggagactc aaatacggca ataggtgtac aatcgatgtt ttcaaacact ggaggatata
    46141 gaaatacagc tactggtttt ggatcattgc gttctaatac ttctggtttt gaaaacactt
    46201 caattggcta tgcatcactt tacaataata taactggaaa tttaaacaca tcagttggtt
    46261 caaatgcatt aaattcaaat acaataggaa gtgaaaattc ggcatttgga aatagttcat
    46321 tgtcatcaaa tacttctgga aatcttaata ctgcgagcgg aaggtctgct ttagcttcaa
    46381 atacaacagg aagtcgaaat gttgctaatg gatcacgtgc cttatattca aacacttatg
    46441 gtttcaataa tactgcaata ggagttttca ctttagaatt taatacttcg ggagataaca
    46501 atacagctgt aggttttgaa agtttaaaaa caaacattac aggatatcat aataccgcaa
    46561 gtggttatca atctttatat tcaaatactt caggctatag aaacacagct tttggatatc
    46621 aatctttata ttcaaataat ggtctttaca atgtagcaat tggttatgaa tctcttaaat
    46681 tgaacacatc aggatactca aacacagcaa ttggatataa ttcaatgtat tccaatgttg
    46741 atggaagatt taatacagca attggtttgt ccgcattata caataatgta aacggatttg
    46801 ataatgttgc aacaggaata caatcgttat ttaataatat tagtggagat aaaaataccg
    46861 ctaatggcag agaatccctg tccttgttaa caactggaaa taacaacatt ggtatcggat
    46921 atctagcgaa tgttccaact gcaactggct ctaaccaagt acgcatagga aacactgcca
    46981 taacttatgc aggaacgcaa gtagcttggt cggtaacttc tgatagacgc tggaaagaaa
    47041 acatccaacc ttctaatttg ggattaaatt ttattaacga tttaaaacca gttttctata
    47101 cacgtaaaaa tgatgaaact aaaaaagtag aatatggcat tattgctcaa gaactagaag
    47161 catcattaga aaaattcggg gcaaacacta acggaattat taccaaagac gatgaaggaa
    47221 tgtacagtgt tcggtacaat gatttattag ctccaatgat taaagccatt caagaacagc
    47281 aagcaatgat tgaagaattg aaagccgaaa tacaaacttt aaaaaataaa aaataatgaa
    47341 aacaacctta atagcattag taagtatttt tttgtttgct ttttcctgta atggagataa
    47401 aacagaaaag gaacaattaa atagtttaga gaaagaagca ttagaagtaa ctaaagaagc
    47461 gggtttaaaa gcggaagatt ttaaagacaa aatagatgaa ctattaactt tagaaatggc
    47521 agcgcaagct tcaggtttac ctgaaactga agctaccaaa aaacacaata caaccgtaat
    47581 ggaaagcgta aaatacgaat ggaaatcaga cagaacaaaa aaagtagcca taaatgggca
    47641 aactataaat gtgcctgcaa ctaattacat cgaattaagt tgggtgaaaa acaccacttt
    47701 acaacaattc aaacacgatt accataatcc aaccgcagaa gaattgcaaa aagcatcttc
    47761 tgcaatggat aaaaaagcta acgaattaaa tgctgaaggt aaagcaacaa aagatcaaac
    47821 cgatgctgcc aattcgattg caaaaggttc gatgtcaaaa tttaaagtag aagaagttcc
    47881 caatcttggc gattatgcag tgtttgtcaa tagcggtatt atgggtgttt ccactagaga
    47941 tttaaaagtt ttttacaaag gactttcgtt tacacttgaa gttgatttat ctgataatgc
    48001 tgccaataat gataaaaaag ccattcaaac agcaaaaatg attattaatc aaaaattaaa
    48061 ataaaatgaa aaaaataatc attctgttct tacttgtgat tggctttaaa gcaaatgctc
    48121 aactcaaatt ttggaataca gacgctagtt caaacatgcc aaaatttgaa gtcgaatacc
    48181 aaaccaaaac caccattttt atgaaagtgg gttatgacgt caaaccatta tatgtgttca
    48241 ataaaacggc accacaagta tataatggcg atggaagaac aaaataccaa atgactgtgg
    48301 aaacttcaga taatatagct cacagaaaat ttgaaattag ttatactttt tatcgccaaa
    48361 caaatcgcta tttaggttac atcaaagcca catatactta taaagataaa cgaccaacaa
    48421 aaatatttga agaaaatttt gaaactgtag ttaatcctta aattaagaaa aatcacaaaa
    48481 tcatgaaaaa aataatcatt ctaacaatcg tattttttac cattcaaact caagctcaat
    48541 tggtgcaagg tgaattaaaa attgatgaaa attcaaaagc tgaactttct tttaaaggaa
    48601 ataaagccgt taacctttat gccgatttta gagaaaacaa acacaaaatc aattttgttt
    48661 ttacctcaac cgatattcct ttaaattctg ataaaaaaga ggtagttcag tttgtgtttt
    48721 caactactgt tttcaaaaac ggtaaaaata ttggaaccat gaaacgttca ccaatgccat
    48781 tttttcctgg tgatatgttt atgccaatag aaactttcga ttttatttcg attttatcta
    48841 atttacagac caatgccgaa gataaaattt ccgaaattcc tgcaggaagt tatgaaataa
    48901 ttattgaagc taaacccata gggacaaaag gaaacattag ttcggctcga ttattcttaa
    48961 aaataaatta aaactattct tcaaacataa aattgtgggt ttatgtttat tcaaaaccat
    49021 tgacttttac cgttgatggt ttttttatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49141 nnnnnnnnat ttataattta ccaataacaa atattgattt ttcaaattta acctcgctta
    49201 ttgttcttta ttgctacgga agtaatttgg ttgaaattga tttaagtaat aatttgtttt
    49261 taaatggctt atattgtgga agtcccaatt tgacttatgt aaacataaaa aatggttggc
    49321 caagtgcttc acaaggcatt caaattttta atagtccaaa cttatcttat ttatgtggtg
    49381 acgaaagtga attaggtcca atagcattaa gtttcgcaac cttatatcca aattgtaatg
    49441 ttggaacgta ttgttcgttt aatcctggcg gaactttcta tacacttacc ggaactaata
    49501 gattagatag taatcaaaac ggatgtgatg ttaatgatat ttcaattgct aatttaaaat
    49561 tcgatgtttt tagtaataca acaggatcat tttatactaa tacttcagga aatttttcta
    49621 ttccgtttca ggctggaaat tattttgtgc ttccaaaatt agaaaatcct acctatttca
    49681 acatttcacc atcacaagta aatttgcaat ttccagacca aacttctcca tacaatcaag
    49741 atttttgtat tactccaaat ggtgttcatt atgatttaga aattacaatt acaagattaa
    49801 aagctcaaat tcctggagca gatgttccgt ataaaataat tttcaaaaac aaaggaaatc
    49861 aaattcaatc tggaactata actttaaact ttaatgatgc aataatggat tttgtttcct
    49921 caaatcctgt tcaaacatct agtacaacca atcaattaat ctggaattat tcaaatttga
    49981 atccatttga aacaagagat atcgaattta ttatgaattt aaattctcct atggaaacac
    50041 cagcattaaa taatggcgac agaattcagt ttactggaaa tattcaacca gagaataata
    50101 atattgtaga tgattttcct gctgataatg atattgcggc taattttaat gttgttaatt
    50161 cagccgatcc aaacgatatc acttgtttag aaggaaattc agttggtcct aatcaaattg
    50221 gaaaatacgt tcattacatg attcgttttg agaacattgg aacggcaaat gctatgaacg
    50281 ttgttgttaa aaatctaatt gatacaagta aatttgatat agcaactttg gttccgcttt
    50341 acagcaatta tgattttgtt actaaacaaa cagctaatca gttcgaattt atttttgaaa
    50401 acatcaatct tccttttgac gatgctaata atgatggata tttagtgtat aaagtaaaat
    50461 taaaaaacaa tttaactgtt ggaacaacat tcaccaacca agctaatatc tattttgact
    50521 ataattttcc aatattaaca aatatagaaa gcacaacaat tgaagctttg agtgctagca
    50581 attttgaaat aaatgatgtt gtaatttctc ccaatccaac atccgatttt ataaatattc
    50641 aatttaaaaa cgcagcagat ttcaaaaccg aaatttatga tatagtagga aagcgaatag
    50701 gaaattttaa aaacacaaac caaattgatg tatccaattt taattcagga atttatataa
    50761 ttaaaattac cgattttgaa tcaaaaacag tcaaaactca aaaattcatc aaaaaataat
    50821 agtagtagtt tttcttaaaa tcctcatttg aatttattca tttggggatt ttgcttttaa
    50881 aatcaatttt ttctatccaa tcattcgtta taaaaatgtt agtatccatt gaaaaaacgt
    50941 agaattgtag taactttgcc tttaatttag acaaaatcaa aataacaatg attaaagttt
    51001 cagacgaagc aagtaagaaa atcgtttcta tgatgcaaga agacggtttt gatgcaacca
    51061 aagattacgt tagagtaggc gtaaaatcgg gtggttgttc gggtttgtcg tatgaattaa
    51121 agtttgataa agaacttgga gaaaacgaca aggtttttga agataacaac gttaaaattg
    51181 ctgttgaaaa aaaatcattc ctttatttag cgggaacaat tttagaattc tctggcggat
    51241 taaacggaaa aggttttgta tttaacaatc caaatgcaca aagaacttgt ggatgtggag
    51301 aatcattttc actttagaat aaaatatgtc aaaatatacc gaagacgact taaaagtcga
    51361 attagaaaat aaagaatacg aatacggatt ttacactgaa ttggactcag aaacgtttcc
    51421 aattggttta aacgaagaaa tagttcgtgc catttcgatg aaaaaaggcg aaccagaatg
    51481 gatgaccaat tggcgaattg aagcttttcg tgcttggcaa gaaatgactg agccagaatg
    51541 ggcaaatgtt cattacgaaa aacccgattt tcaagccatt tcgtattact ctgcacccaa
    51601 aaaagctgat ccaaacaaaa cattagatga tgttgatcca gaattgttag ccatgtataa
    51661 aaagctagga atttctattg atgagcaaaa aaagatgaac aatgtcgcta tggatattgt
    51721 ggtcgattca gtttcggtag ctacaacatt caaaaaaaca ttaaacgaaa aaggaattat
    51781 tttttgttct atctcagatg ccataaaaga acatcctgaa ttagttcgaa aatatcttgg
    51841 aaccgttgtt ccgcaaagag ataattttta tgcagcttta aactcagcag ttttctcaga
    51901 tggaagtttt tgttacattc caaaaggcgt tcgttgtcca atggaattat caacttattt
    51961 ccgtattaat caggctggaa caggacaatt tgaacgcaca cttttgattg ctgacaaagg
    52021 cagttatgtt tcgtatttag aaggttgtac ggcaccaagt cgtgatgaaa accaattaca
    52081 cgcagccgtt gttgaattaa ttgcattaga cgatgccgaa atcaaatatt caaccgttca
    52141 aaactggttt ccaggaaaca aagaaggaaa aggcggcgtt tataattttg tgacaaaacg
    52201 tggaatttgc gaaaaaaacg ctaaaatatc ttggacacaa gtagaaacag gttctgcagt
    52261 aacttggaaa tatccatcat gtattttgaa aggcgataat tcggttggtg aattttactc
    52321 aatagcggtt accaataatt tccaacaagc cgataccgga actaagatga ttcatttagg
    52381 taaaaacact aaatcaacca ttatttccaa aggaatttct gccggaaaat ctcaaaatag
    52441 ttatcgtggt ttggttcgaa taagtgccaa tgccgataat gcgcgtaatt tttcgcaatg
    52501 tgatagtttg ttaatgggaa ataattgtgg cgcacatact tttccatata ttgaaagtaa
    52561 aaatacttct gcaaaaatag aacacgaagc aactacttct aaaataggtg aagatcaagt
    52621 tttttattgc aaccaacgtg gaattccgac agaaaaagca attgctttaa tcgtaaacgg
    52681 ttttagtaaa gatgttttaa acaaattacc tatggaattt gcggttgaag cgcaaaaatt
    52741 attagaaatt tcattagaag gttctgtagg ataataaaat ataaattcga ataaaaaaac
    52801 acattcaaaa aatgttatca ataaaaaatt tacacgcttc agttgaagac aaagaaatat
    52861 taaaaggaat taatcttgag ataaaagcag gagaagttca cgcaataatg ggaccaaatg
    52921 gttctggaaa aagtacactt tcggctgtaa ttgctggaaa cgaaacgtat gatgttactg
    52981 atggttcagt agaacttgaa ggcgaagatt tagcagattt ggcaccagaa gaaagagcac
    53041 acaaaggcgt tttcctttcg tttcaatatc ctgttgaaat tcctggagtt tcggtaacca
    53101 atttcatgaa aacagccatt aatgaaacgc gtaaagcgaa cggaaaagaa gagatgccag
    53161 caaatgaaat gctgaaatta atccgcgaaa aatcagaatt attagaaatt gatagaaagt
    53221 ttctttctcg ttcgttaaac gaaggttttt caggtggtga aaagaaacgt aacgaaattt
    53281 tccaaatggc aatgcttgaa ccaaaaatag ctattcttga cgaaactgat tcaggacttg
    53341 atattgacgc cttgaaaatt gttgcaaacg gtgtaaataa attaaaaaac gaaaacaatg
    53401 cggttttagt aatcacgcat taccaacgtt tgttagatta tatcgttcca gactatgttc
    53461 acgtattaat gaatggaaaa atcgtaaaat caggcggaaa agaactggct tacgaactag
    53521 aaaaaaaagg atacgactgg attaaaaatg aatttaatta aaagagttta tagtctcagt
    53581 ctcagttatc agaaactgcg aatgaatact gcgaatgaat actgaaaaaa tggatttaaa
    53641 agaaaaatta atatcatctt tcatggcttt tgaagaaagt attgatgtaa accacgagtt
    53701 acatgatgtc agaacttcgg ctataaaaaa ctttgaaaac aaaggctttc caaccaaaaa
    53761 agaagaagct tggaaatata cttcgttgaa ttcaatcatt aaaaatgatt attcggtatt
    53821 tccaaaacat gaaaatacta ttgaatttaa agatgttaaa aagtttttta ttcatgaaat
    53881 agatacctat aaagtgatat ttgtggatgg aaaattttct tcatatttgt cttcaacaac
    53941 ccatgatggt ttggacgttt gcttgatgtc atcggcatta aacaagccaa aatataaaat
    54001 gattattgat gaatatttca ataaaattgc aagtaaggag gaaagtttaa cgagtttgaa
    54061 tacggctttt gccaatgaag gtgcgtacat aaacattcca aaaagtaaag tagttgaaaa
    54121 accaatcgaa atcatttatt tttcaacagg aaaagaaaat gcattgatgg ttcaaccaag
    54181 aaatttggta atcgttggcg aaaatgctca tgttcaaatc attgaaagac atcaaagttt
    54241 gaatgaaaat ccggtattga ccaatgcggt aactgaaatt tttgctcaaa aaagagctat
    54301 agtagattac tacaaaattc aaaatgacat gcaatcggca aacttaattg acaatactta
    54361 tattgctcaa aaacaacaaa gccatgcatc agttcatacg ttttcttttg gcggaaatat
    54421 taccagaaac aatttgaatt tctatcatca aggcgaacat attgaaagca cgttgaaagg
    54481 tattaccatc attggcgata agcaacatgt ggatcattat accttagtgc aacacgctac
    54541 gccaaattgc gaaagtcatc aaaactataa aacgatttta aacgatagcg caacaggggt
    54601 ttttaacggt aaaatttttg ttgaaaaaga agcacaaaaa accgacgctt ttcaacaaaa
    54661 caacaacatt ttactgagtg ataaagcaac aattaatgcc aaaccacaat tagagatttt
    54721 tgcagatgat gtaaaatgtt ctcacggttg tactattggt cagctagatg aaacagcgat
    54781 gttttatatg caacaacgtg gcattccgaa gaaagaagcc aaagcgttat tgatgtatgc
    54841 cttttcaaac gaagtgatag aaagcatcaa aatacccgaa ttaaaacaac gaatcacaaa
    54901 gattatcgcc acgaaattag gtgtgaattt aggttttgat ttgtagagtt taaacgaaaa
    54961 attcgcttcg atatatacaa agagcgcaag attttggtct ttgcgctctt tgtattttca
    55021 ctttgcgctc tttgcggtaa aagatagaaa caacctctct caaaaaccca ttaaaatcaa
    55081 attcaggatc ttccgttaat cccattctaa caattactaa atcttggctt ggaaaaatag
    55141 caaccatttg gccttgataa ccgctacaac gatacatgtc ttttggtaca tctggaaaat
    55201 taccaccagc attcaaccaa aattgtgcac cataaattcc gttggatgta ttggttgggg
    55261 tagccacata ttttgcccaa ttttcatcaa aaatttgttc accattccag tttcctttgt
    55321 gtaaatatag taagccaaat tttgcccaat cacgagttgt tgcccaacca taactagaac
    55381 caacaaaatt tcctgccata tcggtttcaa taattgctga agtcattcca attttatcta
    55441 ataaagagga ataccaaaaa tccaagtatt cttgatgggt tttaaattgt tgtcgcaaaa
    55501 ttccacttaa caaattggtc gttcctgatg aataattcca agtttgattg ggtttcccaa
    55561 ccaaaggttt atcaatttgc gattttgtca tgtcttcatc tataaaaagc atttgggttg
    55621 catcacaaat cttatcataa cgttcttccc attctaaacc cgaattcatg tgtagcaaat
    55681 cgtttatcgt aatgtttttg cgctcatcat tttgccatgc tgcaattggc gcaggtttca
    55741 tgatgtcaat ttttccttga tgttgcaaaa tcccaaaata agttccagta atactttttg
    55801 tcatcgacca acctaaaatt ctggagtttt tattaaaacc agcagcatat ttttcggcga
    55861 tgattttgtc tttgtaaacc accaaaacag aacgggttcg tttgtttttt tctccttttt
    55921 tatcaaaagc aatcgctaca gcattttgta atttttcgta atcaatgttg gcaaaaactg
    55981 tgtctttttg ctcaagattt ccatatggaa aaggtaaatt agaagagatt ttagttcttt
    56041 ttggaacttc ataaggttta gtaacatcaa aatcatcatt aatcaaagtg gcacctaaac
    56101 cttcacgaaa aatagctttg cgttctttaa aaccatatac atttgaagtt acaaattgat
    56161 gagtttcgtc gatttcattg gttgcccaat caacttttgg aatatcatta tcaccatttt
    56221 ggatggtttt aagtgaacga ttgtccaaaa aatgtccaga agccacactt tttgccgaaa
    56281 aaccagaaat caaatccaat tgtggataga tggcaattcg aagataagca agagcaacta
    56341 aaagtagaag tacaaaaaag agaactgttt ttttcatttg aaagaaaatt tgatgcaatt
    56401 taaagttttt tatagataaa ttaaaattac gaatgcgttg attatctttg gtcaaaatca
    56461 ttttaatatg aataaagttt ttgacttatt agtttccaaa aatttcatcc gcaaagaaca
    56521 atatcaagcg ataagtgaat atcaaaaatt ggaattgttt tctataagaa acgaattgtt
    56581 gtttttactt tatatttcta ttttgctatt caccactggt gttggaataa ttatttataa
    56641 aaacatcgat accattggac atacagtttt gttgttttta ttagccctat taacagtggt
    56701 ttgtttttac ttttgttaca aaaaaagtag tggtttttca aaacaagaag ttacttttga
    56761 gaatccactc tatgattatt tggtgctttt taccacaatc ttaatgtgta cttttatagg
    56821 ttattttcaa tataatttga attttcaaaa cagcaattat agcattgcta cattagtttc
    56881 ggcaatggca gccttggcaa tggcttatta ttttgacaat aaaagtgctt taattattgg
    56941 tattactgct ttgggaagtt ttgttggact ttcgttaaaa attcaaacaa ttttcgagaa
    57001 caccattttt aatgaaacaa atttactggt tgcgggaata ttatttgcta gtttactgct
    57061 tgtttggcaa tggttttcgg agaagaatag gctaaaaatt cattttagtt ttgtaatact
    57121 tacgtttgct ttgcacctaa tggcaatatg ttgttttgct ggttcttcgc aagacatata
    57181 ttggattttt tatctaatcc ttttaggcgc agcaatttat tatttttatc aaaaaagcat
    57241 cacattcaaa gccatttcat ggtatgtttt tgctttattt tatggatata ttggtttaaa
    57301 tgtgttgatt ttgagaataa ttgatgcagt aaagatggat caaattttcg aatttttagt
    57361 gttcttaacg ccattttatg taattggctc tattgtattg tttattaaaa tgattaaaaa
    57421 ttttaaaaag aaaaccaatg ttagctagca attttaatac catacaaaac cattttttac
    57481 tcgaagaaat ggagaaatta caatcgcaag attttgtgtc taaagctcag ctgagtgaaa
    57541 ttaagaacaa cacgaaaacc acaaaaacca attcaaacat tttgatgcga ataggtttct
    57601 ttcttttagg aaatttttta atttcttcca ttttaggctt ttgcgcattg tttttaagtg
    57661 tgttaggaga tcaaaatgca attgcttttt gttttttgtt ggcatcaatt ggatgtattg
    57721 tcgcttcaga acttatttac gacaaaaact attttgctta tggttttgac gattctttta
    57781 tcttgtcaat cacactattt ttctgtgttg cactagggct tttcacagaa aatgttatcg
    57841 cagtattgtt tgtttttgtg ctaatttcaa gtttttgttc cgttagatat gttcatgttc
    57901 catcgacatt ttttgcgtta atgggattga taggattg
//
LOCUS       DAOOBH010000005        51543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_803_length_51543_cov_7.164737, whole genome shotgun sequence.
ACCESSION   DAOOBH010000005 DAOOBH010000000
VERSION     DAOOBH010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 51543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51543
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_803_length_51543_cov_7.164737"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="PK218_01315"
     CDS             <1..150
                     /locus_tag="PK218_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPW97181.1"
                     /translation="KHWSGRNSENMVTVFPKGDYKPGDFVLVKVTDCTTATLIGEAVG
                     YSEMN"
     gene            205..591
                     /locus_tag="PK218_01320"
     CDS             205..591
                     /locus_tag="PK218_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPW97182.1"
                     /translation="MDNNLEYYYKKDENYTIIGLCMEVHRILGPGLLEIVYKDALELE
                     FRNNKIPFEREKEFEIEYKGIKLPHKFYADFIVYDDIILEIKSVKEINNEHIAQTLNY
                     MKLADSPIGIIANFQNKSLASKRLVI"
     gene            644..1900
                     /locus_tag="PK218_01325"
     CDS             644..1900
                     /locus_tag="PK218_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPW97183.1"
                     /translation="MESVQSIKQRFEIIGNDPKLNRAIEKAIQVAPTDISVLVVGESG
                     VGKESIPRIIHSLSHRKHGKYIAVNCGAIPEGTIDSELFGHEKGAFTGATSTREGYFE
                     VADGGTIFLDEVGELPLTTQVRLLRVLENGEFIKVGSSQVQKTNVRIVAATNVNMFDA
                     IEKGKFREDLYYRLSTVDIPLPPLRDRKDDIHLLFRKFAADFAHKYKMPAIKLREDAI
                     AFLQKYRWSGNIRQLRNVAEQISVLETNRDITLATLQSYLPTESSNLPSVISGKKSDS
                     DFATERDILYKVLFDMKSDLNDLKKLTLELIKNGTKIQDINPSLIQKVYGSNDSEISF
                     EEEPRRSSLPIQSPVIQDNYQDDEDQNFLDAETVEEEEVLKLEQKEIELIKKSLERHN
                     GKRKAAADELGISERTLYRKIKQFDL"
     gene            1936..2775
                     /locus_tag="PK218_01330"
     CDS             1936..2775
                     /locus_tag="PK218_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97184.1"
                     /translation="MKNIISLFVIIIVLVSCNNDDSTNSTVINPNLLQRVDFFSGTNY
                     EKRWCFNSNGFLEEIKDANGILLEKFVYNSNNQVIQNLIYENGIVSQTLNISYSSNNI
                     ISEINGNPFNYNSTENKYYDSDGLFYNYNCNLNSEFLMKSSSAINDDPDYYYEYTYNT
                     IYIENNMFSFSSYDTNTFQEIFTFYEFDNNVNPLKQALMPVFKVKSITNPKFYYDSLS
                     SANNIISQGYLDVDPEYHTYDYTFNSNNLPVNQTRNDYYLDVFENSIESKHYYYQGDI
                     IPN"
     gene            2806..3309
                     /locus_tag="PK218_01335"
     CDS             2806..3309
                     /locus_tag="PK218_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPW97185.1"
                     /translation="MKFTKYILLLFVVLVINSCSVYNFTGTGKIDAKTFQVNYFLNNA
                     ELIEPGIERTFTLRLQDLIQNQTNLNLTNTNGDLVYEGEIVRYNVTPMTATADIRAAQ
                     NRLTIAVNVRFTNKNKEEDNFEKQFSFYDDFPADTPLAGSQLTSSLDVIFERITQDIF
                     NASLAKW"
     gene            3353..4177
                     /locus_tag="PK218_01340"
     CDS             3353..4177
                     /locus_tag="PK218_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW97186.1"
                     /translation="MNLTDYTYLLNKPDAINEKHAESLEKVMDEFPYFQSARVMRLKH
                     LYNQDSYKYNHALKVSAAYTTDRSMLFDFITSDDFVAVQKGLYEKKLQELMNIHVVES
                     EIVVVNSGYKSSNPLEQSILNSIQQSNPEESLAIEKLEIGKPLEFSKDEKHSFEEWLQ
                     LARFSPIIREDKEADNDKPISEEKKKKIDLIDKFIETNPKIPAMDKEKSIAIKENREE
                     DTSYLMTETLARVYLEQKKYTKAIQAYEILILKYPEKSSFFADRILDIKILQQNNN"
     gene            4180..4539
                     /gene="secG"
                     /locus_tag="PK218_01345"
     CDS             4180..4539
                     /gene="secG"
                     /locus_tag="PK218_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPW97187.1"
                     /translation="MSTFSIFLVLITIVCFLLIVVIMVQNPKGGGLSSSFGGSAQMGG
                     VQKTSDFLDKSTWTLATVLIVLILLSSLSFSGALSDSSSKIIDETEKAAPAKPADTKT
                     VAPATSTTPASPAEGQK"
     gene            4682..4957
                     /locus_tag="PK218_01350"
     CDS             4682..4957
                     /locus_tag="PK218_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPW97188.1"
                     /translation="MALNIKPLSDRVIVEPAAAETQTASGIIIPDTAKEKPQKGTVVA
                     AGKGKKDEPMTVKIGDTVLYGKYAGTELKFDGKDYLIMREDDILAII"
     gene            5015..6646
                     /gene="groL"
                     /locus_tag="PK218_01355"
     CDS             5015..6646
                     /gene="groL"
                     /locus_tag="PK218_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989998.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPW97189.1"
                     /translation="MAKDIKFDIEARDGLKRGVDALANAVKVTLGPKGRNVIISKSFG
                     GPTVTKDGVSVAKEIELKDPLENMGAQMVKEVASKTNDLAGDGTTTATVLAQAIVKEG
                     LKNVAAGANPMDLKRGIDKAVEAIVADLGKQAKEVGSSSNKIKQVASISANNDEVIGD
                     LIATAFGKVGKEGVITVEEAKGTDTYVDVVEGMQFDRGYLSPYFVTNPEKMNVELENP
                     YILLYDKKVSSLKELLPVLEPVAQSGKPLVIIAEDVDGEALSTLVVNKLRGALKIAAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEESGYTLENATLEMLGTAEKVTIDKDNTTLVN
                     GAGSADLIKNRVNQIKAQMETTTSDYDREKLQERLAKLAGGVAVLYVGAASEVEMKEK
                     KDRVDDALHATRAAVEEGIVAGGGVALLRAKKALDKVKAENADEATGIQIVSKAIESP
                     LRTIVENAGLEGSVVVAKVSEGKDNYGYNAKTDKYVDMLKAGIIDPKKVTRVALENAA
                     SVSGMILTTECALIDIKDENAGGGMPMGGGMPGMM"
     gene            complement(6709..7389)
                     /locus_tag="PK218_01360"
     CDS             complement(6709..7389)
                     /locus_tag="PK218_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPW97190.1"
                     /translation="MNNFDWMQLLNPEFYITMTFGGIQVGLWIVLFIVFAETGLFAGF
                     FLPGDSLLFLAGIYSRSLVENFTIIESDFVNVFFLALLVAISGIFGNMIGYWFGSKSG
                     YYLYKKEDSFWFKKKYLLQSKDFFERYGGKAIIFARFLPVFRTFAPIVAGIVFMDKKK
                     FMFYNILSSFLWSFTLIFSGHYLYGLFLTEFNIDLKHHIEKIIIIIILISTVPVILKM
                     FKKEKSKA"
     gene            complement(7507..7848)
                     /locus_tag="PK218_01365"
     CDS             complement(7507..7848)
                     /locus_tag="PK218_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal transporter"
                     /protein_id="HPW97191.1"
                     /translation="MKNIIIGMMLFLVSFSIQAQEKQNKNAKYDITVNGNCEMCKKRI
                     ENAAFSVSGVKSAEWHIDDHVLHLIINEKKCSIIDVKKAIAEVGHDTDEIKATEEQYQ
                     KLHGCCMYERE"
     gene            complement(7875..9881)
                     /locus_tag="PK218_01370"
     CDS             complement(7875..9881)
                     /locus_tag="PK218_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW97192.1"
                     /translation="MKKTILFFALLFFTNSFSQKELEEVKIKVQQKGLKKSLKGASNT
                     TLVTSKELLKAACCNLAESFETNPSIDVNFSDALTGTKQIKMLGLTSPYIMITEENIP
                     SVRGASQAYGMSFTPGTWVESIQITKGAGSVVNGYESISGQINTELLKPIDDILFFLN
                     AYGDNSSRFELNTHFNKKVNDKWATSLLVHGSGRFGHHDSNNDGFLDNPQGKQLNVTN
                     RWQYANQETGWVSFLNARFMRDEKLSGEIHFDPNRDKLTTNHWGSEIETERLDFSSKI
                     GYVFKDMPYQSIGFQNAFSLHNQQSYYGLNQYDIKQQSFYSNLIFNSIIDNTRNKFSA
                     GINFAFDNYSEFVNTIDVSRIDNSIGAFFEYTFDDSDKFSYILGGRIDTHNRLGTFFT
                     PRLHVKYNVFENSVLRLSAGRGKRAANIFAENQTLFASSRQFSVLDTNGKIYGLNPEI
                     AWNYGVSFTQKFKFFSKSADITLDFYRTDFQNQAVVDVMNSPQQVLFYNLKGKSFANS
                     LQFDFNIELKKHLNLRTAYKYYDIATDYISGTFQRPLQAKHRLFANIEYETHILDKGQ
                     QWKFDFTTNWLGKQQLPYTASNPEEDRFPEFSPSFAVMNFQVTRTFSSIFEMYLGGEN
                     IGNYRQDRAILDSDNPFGTNFDASIVYAPIFGNMYYAGLRFKIK"
     gene            complement(9936..10352)
                     /locus_tag="PK218_01375"
     CDS             complement(9936..10352)
                     /locus_tag="PK218_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97193.1"
                     /translation="MIFKKQLSILIAFLVLVSNSGLAFNVHFCEGKIASITSVFSKEE
                     VCDMPIVVEKTCCAKVETTHSKCCSDKEVTLKDDVEKVLIKISFDANFVIVFNEFTPL
                     EFNQVFSKKAKEKIVYSCDANAPPLYKLYCQYTFYS"
     gene            complement(10406..11761)
                     /locus_tag="PK218_01380"
     CDS             complement(10406..11761)
                     /locus_tag="PK218_01380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW97194.1"
                     /translation="MKIKFIGGAGTVTGSKTLIESNGIRILIDCGLFQGIKPLRELNW
                     EPLPVLPSTIDFVLLTHGHLDHCGWLPRLVNQGFSGKIFCTSPTAAISKLILLDSAKI
                     QEEEAEKANKGKYSKHDLAEPLYDVSQAEKVFPHFRIIKENEEINLDAEISAVFKNAG
                     HIIGACSIELKIENKILVFSGDIGRDNDVLMYPPTKPKKADYVFLESTYGNRVHPTED
                     TKLLLEMFINNTYHNHGTVLIPSFAVERVQTLMFLLWQLKKEQKLPDIPLIIDTPMGI
                     NVLDVFQKNTSWHKLNAHECEEMCKIFTLISDYKETIDTIYNPKPKVVIAASGMITGG
                     RILSYLEEYIGKPETSVIIVGYQAEGTRGRKLLEGANEIKIYGKYYSVNAKILEVEGL
                     SAHGDQNDLINWLSDLETKPQKVFLVHGENQPADELRIKIKEKLGIECSIPLMGQEIE
                     I"
     gene            complement(11763..12593)
                     /locus_tag="PK218_01385"
     CDS             complement(11763..12593)
                     /locus_tag="PK218_01385"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HPW97195.1"
                     /translation="MKIIKQSGQVVDFNIQKLKHSLLKSGASIETVEKVLAQIESQLY
                     EGISTRKIYKLAFSLLKKSAKSHAARYNLRTAIQQLGPAGFYFEKFIAELFSSEGYSS
                     KTNLFLNGKCISHEIDVVIKKDELISIIECKFHNTNDKVTDVKVPMYILSRFNDLKSQ
                     KHSIFSNNDEVSKCIIVTNNRFTSDAIAFGTCSGLQLLSWDYPAENCLRKKVDRNGLY
                     PITCLTTISSAEKSKLLELGLILVSHLIKTPDLLSKIGISQKRSVNILKEATELCNYI
                     "
     gene            complement(12631..13074)
                     /locus_tag="PK218_01390"
     CDS             complement(12631..13074)
                     /locus_tag="PK218_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase F system-associated protein"
                     /protein_id="HPW97196.1"
                     /translation="MLAFLKKYKSKVIYSTLLILVLVLIRMFENQLFYDPFLNYFSSE
                     YANLPFPKFNIFKLFFSLGFRFYLNMVISLGLLYVLFDDRKIVKFSIFLFMLLGSILL
                     ISFFFVLTFFAEESKMTLFYIRRFLIQPIFILLFIPGFYYQKIAK"
     gene            complement(13049..13606)
                     /gene="xrtF"
                     /locus_tag="PK218_01395"
     CDS             complement(13049..13606)
                     /gene="xrtF"
                     /locus_tag="PK218_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase family protein XrtF"
                     /protein_id="HPW97197.1"
                     /translation="MKNLLQQYKPFLLFLGKFFLSYLILTILYQSYLNQFDVKNAEVD
                     GFTKSVAEQTEWVLSLIDNHSYTTKHLTEPSIKLFYKNKYISRVIEGCNGLSVMILFV
                     AFVIAFSGKFKNTLLFLLFGIVIIHVLNVSRIALLSMALYFYPQFEHFLHGVLFPLII
                     YGVVFVLWVIWVNNYSSYASVSKKI"
     gene            13678..14133
                     /locus_tag="PK218_01400"
     CDS             13678..14133
                     /locus_tag="PK218_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPW97198.1"
                     /translation="MELDDLKPEVATILNDNKIDRDTKLKNLCQFLSDSVSYYNWVGF
                     YFANHETKTLHLGPYVGAETDHTVIPFGKGICGQVAESNANFVVPDVAAQDNYIACSF
                     TVKSEIVVPLFVHGKNIGQIDIDSHVIDPFTETDERFLEFVNKEVAKLF"
     gene            14191..15756
                     /locus_tag="PK218_01405"
     CDS             14191..15756
                     /locus_tag="PK218_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HPW97199.1"
                     /translation="MKLNINNKFTNELPADALDSNETRQVAGAAFSYVNPVKPKKPCL
                     IHVSKELAESVGISLEEIKSDAFLTIFSGKTILEKTKPYAMAYAGHQFGNWAGQLGDG
                     RAITLFEAIHNNRSTMFQLKGAGKTPYSRSGDGLAVLRSSIREHLCSEAMFHLGVPTT
                     RSLALFTSGDMVLRDILYNGNPAYEKGAIVCRTASSFIRFGNFELFASQNDIKNLQLL
                     TDYTIKHYFSEINSVGKEKYLEFFETVADKTREMIVHWQRVGFVHGVMNTDNMSIHGI
                     TIDYGPYGWLEDFNPNWTPNTTDNQYKRYRFGNQPQIAQWNLLQLANALFPLIEEAKP
                     LEKILNDFPIKYEADYIEMMHSKLGFEFSSEASKNIINLLTQNLQLTETDMTIFFRSL
                     SLVSKVDSVENAFEKIATSFYKLEEVKDSVYDTWMYWFTLYINQLKLENQSDSERKKT
                     MNLVNPKYVLRNYMAQLAIDAADKEDYNLVNELYEMLLKPYNEQKQYEKWFAKRPDWA
                     RYKVGCSALSCSS"
     gene            15828..16406
                     /locus_tag="PK218_01410"
     CDS             15828..16406
                     /locus_tag="PK218_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HPW97200.1"
                     /translation="MKKLLCEIKKCKVCEPYLPLGANPVLYVSKTSKIIIIGQAPGKI
                     VHNTGIAWNDKSGDTLRKWLGVDKETFYDDKQFATVPMGFCYPGTGKNGDFPPRKECF
                     PLWHHKILSEIEGNPLILLIGQYAQKKYLNKKGKSNLTETVKSFHDYLPNYFPLPHPS
                     PRNNIWMKKNFWFEKDVLPELKKRIAGLIKNN"
     gene            16645..16914
                     /gene="rpsO"
                     /locus_tag="PK218_01415"
     CDS             16645..16914
                     /gene="rpsO"
                     /locus_tag="PK218_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPW97201.1"
                     /translation="MYLSKETKAEIFAKHGGKAENTGSAEGQIALFTFRISHLTEHLK
                     KNRHDYNTERSLVLLVGKRRSLLDYLKKKDITRYREIIKELNIRK"
     gene            17067..19223
                     /locus_tag="PK218_01420"
     CDS             17067..19223
                     /locus_tag="PK218_01420"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPW97202.1"
                     /translation="MIPQLFVESIDLGDGRNITIETGRLAKQADGSVVVRIGKTVILG
                     TVVSARKASPGIDFLPLTVDYREKFAAAGRFPGGFFKREARPSDSEVLTMRLVDRVLR
                     PLFPSDYHAEVQVMIQLMSHDEEVMPDALAGLAASAALALSDIPFETLISEARVGRID
                     GKFIINPSRAQLELSDIDMMIGASRDSIAMVEGEMKEISEKEMVEAIKFAHEHIKVQI
                     DAQERLAAAFGKKEVRTYEGEREDEAIYAKVKAASYDKIYAIASKGSAKHERSAAFDE
                     VKEEVKALFTEEELAENGDLVGKYFYKVNKEAVRNVTLDLGTRLDGRKTTEIRPIWCE
                     VDYLPSVHGSALFTRGETQALATATLGTSREANQIDSPSEQGEEKFYLHYNFPPFSTG
                     EARPLRGTSRREVGHGNLAQRALKNMIPADCPYTIRVVSEVLESNGSSSMATVCAGTL
                     SLMDAGVQMIRPVSGIAMGLITDGDRFAVLSDILGDEDHLGDMDFKVTGTSEGITACQ
                     MDIKIDGLKYEIMEQALAQARDGRLHILGKLTETLAQPRAEVKDHSPKIITRRIPNAF
                     IGALIGPGGKVIQELQKATGTTIVINEDPVTEEGIVEILGTDPAGIATVMAKIDSIIF
                     KPTVGEAYEVKVIKMLDFGAVVEYTQAPGNEVLLHVSELAWERTENVTDVVNMGDVFM
                     VKYFGLDPKTRKEKVSRKALLPRPPREENKITPKGE"
     gene            19332..19988
                     /locus_tag="PK218_01425"
     CDS             19332..19988
                     /locus_tag="PK218_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97203.1"
                     /translation="MKLNIEIKSIYTLDEIPNYWTNQDYVALLAAFGFADGNTIKPEN
                     LTEMLFMAIADYEPNEAAQIVLTYKLSNDLNEGQIDQLSNEMLLDKVCEEYPEIHLHK
                     HLFSINQLLYKAFNGKFPNAKASSIQFSVLGNDSNELSKSDILKAFNSGLNESNIIKR
                     LFGEPMTTGAKFPEAESLIWELNTKEDSTFQLITSEYLIDRESFINMEFDGEILNEEE
                     "
     gene            20063..21799
                     /locus_tag="PK218_01430"
     CDS             20063..21799
                     /locus_tag="PK218_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPW97204.1"
                     /translation="MSSHKKLKEFASTAICGNDITSSCLYVSALAIMYAGQYAWISLL
                     IVAGVLFLFRKIYGEAVGALPLNGGAYNILLNTTSKSNASIAACLTILSYMATAVLSA
                     SEAMHYLHSLVSSVPVFWATIGLLTFFLVLIMSGIKESSNVAIFIFVFHLASMIFLIG
                     SGIWFAYSNNFDIMSINFSQPIEGSIPVALFLGFSTAMLGISGFESSANFVEEQEPGV
                     FRKTLRNMWIAVSVINPLMALVIIGVVPLIEVGAHKETLLSHLGNLTGGSNLATLISI
                     DAVLVLSGAVLTSYVGVSGLMKRMSLDRILPQALLKENKSGSSPRILIVFFLLCVSVL
                     FITNGELGPLAGVYTMSFLAVMAYFGFGNFLLKIKRTKLPRPEYAQPYAVAIAILAII
                     IAIYGNILMHPEYLVVFLQYFVPSIVLILLLLNRKFVLQYLLVVIKSFIDSFRKHARI
                     GEVLLTKKINRLTQQEFVYFSKGDNISQLNKAILYVQENEITKKLKIVTIVNEDKQVT
                     DEFMSDFNALDRAYPEIKLEYIQMEGEFGPKLIETLSEKWKIPSNFMFISSPGNRFSY
                     RVSELNGVRLIM"
     gene            21896..22759
                     /locus_tag="PK218_01435"
     CDS             21896..22759
                     /locus_tag="PK218_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW97205.1"
                     /translation="MRQLKITKQVTNRETASLDKYLQEIGKVDLITADEEVELAQKIK
                     AGDQRALEKLTKANLRFVVSVAKQYQNQGLTLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKIGSINKINKMYALLEQSNERPPSAEEIAK
                     ELDMTVNDVKESMKNSGRHLSMDAPLVEGEDSNLYDVLRSGESPNPDRELIHESLRTE
                     IERSLETLTPREADVVRLYFGLGDQHPMTLEEIGETFDLTRERVRQIKEKAIRRLKHT
                     SRSKILKTYLG"
     gene            22971..23633
                     /gene="rpe"
                     /locus_tag="PK218_01440"
     CDS             22971..23633
                     /gene="rpe"
                     /locus_tag="PK218_01440"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPW97206.1"
                     /translation="MKNTLIAPSVLAADFANLQRDIELINTSEADWFHIDIMDGVFVP
                     NISFGMPVLQAISKHAKKTIDVHLMIIDPDRYIKTFAQLGSNILTVHFEACTHLHRTL
                     QAIKAEGMKAGVALNPHTNVDLLEDVIQDIDLVCVMSVNPGFGGQSFIENTYSKVEKL
                     KALIQRKNASTLIEIDGGVTSKNAKQLVDAGADVLVAGSFVFTSENPIQTISDLKKVA
                     SN"
     gene            complement(23630..24454)
                     /locus_tag="PK218_01445"
     CDS             complement(23630..24454)
                     /locus_tag="PK218_01445"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HPW97207.1"
                     /translation="MEIVGIIILLLFGILVIGAFLLYSIMFVLSLLDDIWISTFDRPL
                     YVHLYLMPKKISEKERFLLIQNFDFYNKLSEKHKKYFHHRIAVFQKNYQFIARGDFQM
                     NKEAQILISATYVMLTFGMRKFIVDVFDKIIIYPEEYFSTQTEDYHKGEFNPRMKAVA
                     FSWKHFEAGCQISNDNLNLGIHEFSHVIHHHSLRNNDGSSLSFKKHFETIIKEVNHPP
                     NRQRLVDSNYFRIYAYTNQFEFLSVIIEHYFETPQQFKNEFPELYANVSKMLNHKH"
     gene            complement(24496..24569)
                     /locus_tag="PK218_01450"
     tRNA            complement(24496..24569)
                     /locus_tag="PK218_01450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24533..24535),aa:Arg,seq:tcg)
     gene            complement(24647..25096)
                     /locus_tag="PK218_01455"
     CDS             complement(24647..25096)
                     /locus_tag="PK218_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPW97208.1"
                     /translation="MSLSAKVMSEMKEAMKSKDTIALEALRAIKSELLLAQTASGSKE
                     ELAEADEIKILQRLVKMRKDSAEIFTKQNRSDLAEPELAQIAVIEKFLPAQMSEAEIE
                     AIISKIITETGASGIAAMGKVMGAASTQIGGQAEGKVISTIVKKLLT"
     gene            complement(25236..27188)
                     /gene="ftsZ"
                     /locus_tag="PK218_01460"
     CDS             complement(25236..27188)
                     /gene="ftsZ"
                     /locus_tag="PK218_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPW97209.1"
                     /translation="MISNSEFGNISFDLPKNQSNVIKVIGVGGGGSNAINHMFKQGIK
                     GVDFIVCNTDSQALQNSAVPNKIQLGVNLTEGLGAGANPDVGQQSAIESIADIEKMLD
                     TNTKMVFITAGMGGGTGTGAAPVIAQLAKERDILTVGIVTIPFQFEGKVRSEQALIGV
                     DRLRKQVDSLIVINNNKLREVYGNLGFKAGFSKADEVLATASRGIAEVITHHYTQNID
                     LKDAKTVLSNSGTAIMGSSIASGENRAKEAIISALDSPLLNDNKITGAKNVLLLIVSG
                     SNEITIDEIGEINDHIQAEAGYNANIIMGVGEDESLDESIAVTIIATGFDVEQQNEIV
                     NSEPKKIIHSLEDEQKLVRDLTQKSVDIFQSKFDSQVEEKEEKIVFELIEEEVENTTV
                     YTNEVEVVDDANELIAMTQFINNLDVTFEIVSPIKDIDFRITTPETKEIKVVEAQNVS
                     KEEEQITFSFETPNDVKNEIDDTRFVFDLSEETKNIVVNEPVQFVSVTELNEEGVVKY
                     SLEEYMELENDLMNSKPVAAILKVEEPVDEDLNIKIKDIEETKTPTFGQSFEDVSPVE
                     MTIEETLKLRADERRRKLKEFNYKFHNNPSKVEELEKEPAYKRSGIDLNENTNANSTS
                     RMSLGTDSNNDSQLRSNNSFLHDNVD"
     gene            complement(27317..28696)
                     /gene="ftsA"
                     /locus_tag="PK218_01465"
     CDS             complement(27317..28696)
                     /gene="ftsA"
                     /locus_tag="PK218_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPW97210.1"
                     /translation="MEKENIAVGLDIGTTKIVAMIGKKNEYGKLEILGIGKAKSLGVA
                     RGVVNNITQTIQSIQQAVLEAENNSGYKINDVVVGIAGQHIRSIQHTDYVIRTNPDEV
                     ISGKDIQLLIDQVNKLAMLPGEEIIHVLPQEFKIDGQSEIKEPIGMYGGRLEASFHVV
                     VGQAASIRNVGRCIQSSGIELSGLTLEPLASADAVLSQEEKEAGVALIDIGGGTTDLA
                     IFKDGIIRHTAVIPFGGNVITEDIKEGCSIIEKQAEALKVKFGSAWPGENKDNEIVSI
                     PGLRGRDPKEISLKNLSKIIHARVVEIIEQVYTEIKAYGHEDPRKKLIAGIVLTGGGS
                     QLKHIKQIVEYITGMDTRIGYPNEHLAGNSSQEISSPLFATAVGLVMNSIANDTVSAY
                     RIELKDALTEAPRQVHVPVPEQKVEEVPNEVFEDEKVIPKETSGEKIKKSFFDKYVEK
                     IKDFLDNAE"
     gene            complement(28704..29426)
                     /locus_tag="PK218_01470"
     CDS             complement(28704..29426)
                     /locus_tag="PK218_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HPW97211.1"
                     /translation="MKILSWTNIRLLLMFFLVIFLYSFTSYRNNHRKIVKVEVKILDN
                     NTPFIKSETVNKLLIEKNNNAKNFTKETLVLNKLENSINKQLLIQKADVYITVDGVLK
                     AVVKQKTPLGRICAENGSFYIDYEGNKMPFSDNYTARVPLISGDITGVKKEKLAQVLR
                     TIYEDEFLQKNIIGVQVLPNGSLILENRNYDFKIDFGRTINIKRKFDNYKAFFQKAVS
                     DSTLIKYKMINLKFTQQVVCIK"
     gene            complement(29423..30763)
                     /gene="murC"
                     /locus_tag="PK218_01475"
     CDS             complement(29423..30763)
                     /gene="murC"
                     /locus_tag="PK218_01475"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPW97212.1"
                     /translation="MNLNQIHNVYFIGIGGIGMSALARYFQNIGKNVSGYDKTPSALT
                     NELIAGGMNIHFEDNISLIPVNYFVENTLVILTPAVPITHSELNYFLEREFIVKKRAE
                     VLGLITKDTFCFAVAGTHGKTTTSSILGHILYESGADVTAFLGGIVENYNSNLIGSGK
                     SISVVEADEFDRSFLHLHPNIACVTSMDADHLDIYGDASSIESSFREFADKVSDKSKL
                     IVPKGLPLEGLTVAINDEATFTATNIRIENSTYVFDVISPSETIENIAFNLPGHHNLM
                     NALMALAMAKTFGTPTNDIAKALASFKGVQRRFSYQIKTERLVYIDDYAHHPTEIDAV
                     HQAVRELYPNQKVLAVFQPHLFSRTKDFADDFAEKLSRFDEVLLLDIYPARELPLEGI
                     TSSWLLSKIENQNKKLVSKFELIPTILKSDATVIVTIGAGDIGELVPSIKKALS"
     gene            complement(30763..31854)
                     /gene="murG"
                     /locus_tag="PK218_01480"
     CDS             complement(30763..31854)
                     /gene="murG"
                     /locus_tag="PK218_01480"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPW97213.1"
                     /translation="MKKLKFIISGGGTGGHIYPAIAIANELKLRFPDAEFLFVGAKDK
                     MEMQKVPQAGYKIEGLWIAGLQRKLTFQNAMFPMKLMSSLWKSRNIIKKFKPNVVIGT
                     GGFASGPLLQMANSANIPTVVQEQNSYPGITNKLLSKKANTICVAYENLERFFPKDKI
                     VFTGNPVRQDLLDISNKRNEAIEHFKLKSEKKTLLVLGGSLGARRINQLIESKIFDIV
                     GKDIQVIWQCGKFYFDEYKHYSDEKNVQVTDFIDRMDLVYATADFIISRAGASSVSEL
                     CLVGKPTIFIPSPNVAEDHQTKNAIAIVEKNGALMIKESELDEKFDEVFQNLISDEKL
                     QNALSKNCKELAKPNATKDIVEEIIKLIK"
     gene            complement(31857..33140)
                     /locus_tag="PK218_01485"
     CDS             complement(31857..33140)
                     /locus_tag="PK218_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPW97214.1"
                     /translation="MKDFINSLKGDKVIWAFVALMAMLSFMPVFSASSNLAYMRHGSG
                     NAFTYLLKHAAQVFVGFIILYNVHKIPYHYYKRLSWIFLPVVWILLGYTLLKGTMIDG
                     ANASRWIQIPFIGITFQTSTLAAIVLYIYVAQYLSKKREMPITFKASLFELWTPVFVT
                     LMLILPANLSTAALIFSMVLMLVFIGKYPLKYIGIILGAGIVGLTFFILVAKTFPDAF
                     PNRVDTWISRIDNFASDKPDEDDYQIEKAKIAIASGGVYGLGPGKSVQKNFLPQSSSD
                     FIYAIIVEEYGLFGGLFVLGLYLLLLFRFVVAAHKSTTLFGKLLVVGLGFPVIFQAMT
                     NMGVAVELLPVTGQTLPLISSGGTSIWITCIALGIIINVTKKEEEIAEEEAENAKREA
                     ALKKLVDKQIEEDEREEDDFSIEENQNPLNVVLNK"
     assembly_gap    33213..33312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<33313..34259)
                     /gene="murD"
                     /locus_tag="PK218_01490"
     CDS             complement(<33313..34259)
                     /gene="murD"
                     /locus_tag="PK218_01490"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPW97215.1"
                     /translation="MQRLVILGGGESGVGTAILGKKKGYEVFVSDFGKIKENYKDVLN
                     QYEIKWEDENHTEDLILNATVVMKSPGIPEKSPIVKKLLEKGIPVISEIEFAAKYTSA
                     KTIGITGSNGKTTTTMLTYHLLKSGGLNVGLAGNVGKSFAWQVAEENFDCYVLELSSF
                     QLDGIIDYKPEIAIITNISPDHLDRYDYKYENYIASKFRITMNQTEDDFLIFDADDEA
                     ISEWLKQNKTKAKLVPFSLSKQFEFGAFIKQDTMNILINEEELEMKIVDISLEGNHNL
                     KNAMAATSVAQLMKIRKETIRESLSNFQGVEHRLEKVLK"
     gene            complement(34261..35487)
                     /gene="mraY"
                     /locus_tag="PK218_01495"
     CDS             complement(34261..35487)
                     /gene="mraY"
                     /locus_tag="PK218_01495"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPW97216.1"
                     /translation="MLYYLFEYLDKTMDIPGAGVFQYITFRSALALILSLMLSTIYGK
                     KIIKFLRNQQVGETVRELGLEGQTQKAGTPTMGGLIIIVATLIPVLLFTKLDNIYVLL
                     LIVTTLWMGTIGFIDDYIKIFKKDKQGLKGIFKVIGQVGLGLIVGSVLYFHPAVTVRE
                     HTNNAVPFNSSEKVINELPSEIKSTTTTIPFTKYNELDYAQILKPFTDEYQNWAWLVF
                     IPIVIFIITAVSNGANLTDGIDGLAAGTSAISVLALGIFTFVSGNIILSNYLNIMYIP
                     NSGEMTVFISAFVGALIGFLWYNSYPASVFMGDTGSLTIGGIIAVLAISVRKELLLPI
                     LCAIFFAENLSVIMQVSYFKYTKKRFGEGRRIFLMSPLHHHYQKKGYHESKIVTRFWI
                     VAIMLAIVSIVTLKLR"
     gene            complement(35577..37040)
                     /locus_tag="PK218_01500"
     CDS             complement(35577..37040)
                     /locus_tag="PK218_01500"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPW97217.1"
                     /translation="MIILKDILYKVAIESVHGSTEMAIEKIEFDSRKVQENDVFVAIR
                     GTVSNGHDFIEKAINLGAVAIVCDTLPEIIVSGVTYIEVKDTNSALAFMASNYYGNPS
                     VNLKLIGITGTNGKTTIASLLYQLFKKAGYKVGLLSTVKIMIDEIEYKATHTTPDSLT
                     INHYLAEMNTVGVAYCFMEVSSHGIHQKRTEGLHFAAGVFTNLSHDHLDYHPTFAEYR
                     DVKKSFFDHLPKTAFALTNVDDKNGAIMFQNTAARKLTYALKSYADYKAQILENQLSG
                     LLLKINESEVWVKLIGTFNAYNLLAIYGTAVELGLDKLEVLRLLSELESVSGRFQFIV
                     SEEQITAIVDYAHTPDALENVLKTINDIRTNNEHLITVIGCGGDRDKAKRPIMAHIAS
                     SMSNKVILTSDNPRSEDPIDIIADMEKGVEVQNQRKTLSIVDRKQAIKTACQLASFKD
                     IILIAGKGHETYQEIKGVRHDFDDMKIVKEFLEQMKK"
     gene            complement(37037..39040)
                     /locus_tag="PK218_01505"
     CDS             complement(37037..39040)
                     /locus_tag="PK218_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPW97218.1"
                     /translation="MAIEDKQISYRIYLVAVSFFLVACAITFKLTEIQWVQGDFYRKL
                     AKERTVKNFIIPANKGNIYSADGSLLATSIPNYTIRFDAVAPKDEDFNANVKALSDSL
                     SLMLGKPSAYYHSELRKAKADKNRYYLLARSLSYTEYLRMKRFPLFNLGTYKGGMITE
                     QKTVREHPIGKIAERTIGYERVTSAGHPDGKGIEWSFRNYLNGKDGKIVKQKIAKGQW
                     KPIRDINEVDPQDGYDVISTIDVYIQDIAHHALLKQLELYEADHGCVVVMETKSGDVK
                     AISNLGRANDGSYYETVNYAVAESHEPGSTFKLVDLIALLDDNKIDTSKVYDTNGGIV
                     TYYGRKVRDSHDGGYGKISLARGFEVSSNTVLVQAVYENYKDDPKEFVDRIDRLGLNK
                     PIGLPFEGEGVPYIPQPSDKKWSGLSLPWMAYGYGVSVTPLQTLALYNAVANNGVMIK
                     PKFVSEIKEWNKVIKKYNTEIINPKICSDETLKKVKAVLGNVVKRGTASKLYSKDFSM
                     AGKTGTAAANYGSNGGADKYYISSFVGFFPVENPKYSCIVVVHKPNTSKNNYYGADVA
                     GPVFKRIAQKIFTDSPSIKEIENLETKIQKVEVAYQEYNDKIQADKKTLPNLKGMAGM
                     DAVAFLENLKIKVKVIGIGKVKKQSILPGELLENIKTITLELS"
     gene            complement(39031..39378)
                     /locus_tag="PK218_01510"
     CDS             complement(39031..39378)
                     /locus_tag="PK218_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HPW97219.1"
                     /translation="MKKGVYGILKARILVNEDATKNWRFIVFLILLAIFMIANNHRFD
                     QKVFRITALTNEVKELRSEFVDRRSELMKLKMESTISQKMEERNIFPSSVPPKKIKVK
                     KIEEKSFFKKIWQ"
     gene            complement(39385..40281)
                     /gene="rsmH"
                     /locus_tag="PK218_01515"
     CDS             complement(39385..40281)
                     /gene="rsmH"
                     /locus_tag="PK218_01515"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPW97220.1"
                     /translation="MEYHNPVLLQETVDGLNVNPDGVYVDVTFGGGGHSREILKRLGP
                     KGKLFGFDQDEDAQANVFDDERFVLIPENFRYIKRFIRFYGVKKVDGILADLGVSSHQ
                     FDVPERGFSTRFEADLDMRMSKKNDLNAFKVVNHYDEANLRRVFLDYGELKIAPALAK
                     TILEARESKPIQTTEELKQVLNKYLPEQFKNKILAQIYQAIRIEVNQEMEVLKEFLEQ
                     SLEILKPEGRLSVISYHSLEDRLVKRFMKNGLFEGEPERDFFGNFSVPFKLIGKMIIP
                     SEAEIKINNRARSAKLRIAEKK"
     gene            complement(40268..40738)
                     /locus_tag="PK218_01520"
     CDS             complement(40268..40738)
                     /locus_tag="PK218_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPW97221.1"
                     /translation="MDSIIGTYECKLDAKGRLMIPAALKKQLAPSLKDGFVLKRSVFQ
                     PCLELYPMAEWTLMMLKINKLNRFVKKNNDFIRRFTAGVKMVEVDELGRLLIPKDLIV
                     FSTISKDIVLSSAVNIVEIWDKELYEKSISGDDVDFADLAEDVMGNLNDDNGIS"
     gene            41141..41905
                     /locus_tag="PK218_01525"
     CDS             41141..41905
                     /locus_tag="PK218_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW97222.1"
                     /translation="MEKIYKKEGRYSYYETGEGTPIVVLHGLMGGLSNFGEVADYFSN
                     NGYKVVIPELPLYTQNILKTNVKAFAKYVKDFITFKGFDRVILLGNSLGGHIALYHSK
                     MYPEKVAGIVITGSSGLYESAMGDSYPKRGDYEYIKNKTEVVFYDPKIATKEIVDEVF
                     AIVNDRIKVIKTLTIAKSAIRHNMAKDLPKMHMKTCIIWGKNDKVTPPEVAEEFHKLM
                     PNSSLYWIDKCGHAAMMEHPQEFNSILHQWLKEVHL"
     gene            41950..42567
                     /gene="yihA"
                     /locus_tag="PK218_01530"
     CDS             41950..42567
                     /gene="yihA"
                     /locus_tag="PK218_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPW97223.1"
                     /translation="MEIHTAEFIISNSEVSKCPKEPLPEYAFIGRSNVGKSSLINMLT
                     NHKNLAKTSGKPGKTQLINHFKINQNWFLVDLPGYGYARVSKKTKEVFQKFITDYFEK
                     REQLVCGFVLIDIRLEAQKIDLEFITYLGEIQVPFCIIFTKADKISKTKIDSHIAAYR
                     KALLANNWEEMPQHFVTSSTEGTGRKELLEFIDSVNQEMFKDNGF"
     gene            complement(42592..42915)
                     /gene="gldC"
                     /locus_tag="PK218_01535"
     CDS             complement(42592..42915)
                     /gene="gldC"
                     /locus_tag="PK218_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HPW97224.1"
                     /translation="MTSEIKFLVELDENRVPEKLTWTAQDGGVELEEAKAMMLSIWDA
                     KAKETLRIDLWTKEMPVDEMKQFFHQTLVAMADTFQRATADEKMAETMKDFCQYFAEK
                     MELTK"
     gene            complement(43035..44000)
                     /gene="gldB"
                     /locus_tag="PK218_01540"
     CDS             complement(43035..44000)
                     /gene="gldB"
                     /locus_tag="PK218_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldB"
                     /protein_id="HPW97225.1"
                     /translation="MKKYFYSIPILFLFFISCDKKSKVEKAVEEIPVQIKVVRFEKDF
                     FDCKPSELQNLKAEYPFFFPENEDDNVWLEKMQNPLWRELYTEVEKKYSNFGKQTTEI
                     EDLFKHLKYYFPTIITPTVYTVIGEMDYNNKAIYANDKLIIALELYLGKEHKFYEFPE
                     YIKQNFEERQMMPDIVSSFSMRTNPPSKEQSLLAEMIYYGKELFLKDLLLPNYTDAEK
                     IGYLPEQIAWCQENESYMWRFFIDESLLYNSDPKLPNRFINLAPFSKFYLEIDNESPG
                     RVGQWIGWQIVRSFMKNNEKVTVQELLKMEYKDIFDRSKYKPKKE"
     gene            44095..44913
                     /gene="nadE"
                     /locus_tag="PK218_01545"
     CDS             44095..44913
                     /gene="nadE"
                     /locus_tag="PK218_01545"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPW97226.1"
                     /translation="MTKKSSINVEKVNQHIVNWLKEYAKKAKVNGFVVGISGGIDSAV
                     TSTLCAQTGFKTLCVEMPIHQDLKQVNRGKEHIEQLRNRFPNVSHAITDLTQVFEIFK
                     SEVPKSDDATKYNLSLANTRARLRMTTLYYHAGINGFLVAGTGNKVEDFGVGFYTKYG
                     DGGVDLSPIADLMKSDVFALAEYLKVPHSIQTAAPTDGLFGDSRTDEEQLGASYDELE
                     WAMVEVEKNKTIEDFFGREKEVFSIYKRLNSANKHKMQPIPICILPKKHNKNDD"
     gene            44983..45612
                     /locus_tag="PK218_01550"
     CDS             44983..45612
                     /locus_tag="PK218_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW97227.1"
                     /translation="MINVCIADNFPVVHFGIKAYFKDHNEINIASNVGNFFMIPDALR
                     NKEIDVLILDLELDGLNSIYEVKSIIKNFPKTKVIIFSSLSEHIYAPNAIKAGCAGYV
                     HKTSKLENLGITIVKVNNGQIILNDDIKKKLALIAKQNKTERLYRKLSNREIEVLRFL
                     SDGKKNNEIAKILNLNEKTISTYKLRLLTKLNVTNLVDLVNKAKTLEIV"
     gene            complement(45646..47634)
                     /gene="dnaG"
                     /locus_tag="PK218_01555"
     CDS             complement(45646..47634)
                     /gene="dnaG"
                     /locus_tag="PK218_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPW97228.1"
                     /translation="MITRETIDKVFETARVEEVIGDFVQLKRAGSNFKGLSPFSEERT
                     PSFMVSPVKQIWKDFSTGKGGSAVTFLMEHEHFTYPEAIRFLAKKYNIEIEETEVSNE
                     DKAIASEKESMYLVSEFAKTYFQETMLNSEEGKAIGLSYFKERGFTTDTIAKFGLGYS
                     PETWDAFTKEALSKGYQLDYLDKVGLTIVKEDKQFDRFKGRVMFPIQSMSGRVLGFGG
                     RILTNDKKAAKYLNSPESDIYHKSKVLYGIFHAKQAIAKQNNCYLVEGYTDVIQMHQA
                     GIENVVASSGTALTPDQIRLINRLTKNITVLFDGDAAGLRASIRGIDLILEEGMNVKV
                     CTFPEGDDPDSFARKNSYDDLVAYLENNAKDFIQFKASILMDEAKNDPIKKADLIRDM
                     VVSISKIPDRIKREIYIQECSRIMDISEQVLLNTLAQIVKKDILEAGKKQKNTENTEQ
                     KAFEVVKNDTQVVAHKVDVIYELERKLIEILLIYGTVDAEFEDVILKTNDTGETIEIP
                     EKNNYKVFQRIYLSLQEDEIELANPIFKDIYSTLIEKYNQEESFSQEQFLQQLSEEQS
                     QIVTDILMNEEKESLHNWETKHIYVKQKEQSISQYVSETIISFREYLINKVIADLMND
                     FSTNEEIDVEELKANINDYNKLKVMLTNKIGRIRSTYY"
     gene            complement(47748..48809)
                     /locus_tag="PK218_01560"
     CDS             complement(47748..48809)
                     /locus_tag="PK218_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97229.1"
                     /translation="MRNFTIYLAVAACLLAGKITAQETFESKARQIAEKIEKITKDEK
                     EALKIEIEEVNKQFENGTITKAEADDKKKKLAEARAIIIENKVAVTHEELKELVQKKV
                     NGKIVSKPSNDSTTVRINNRYVLKFEKDTVSFKKKKDYSERRTTSQFVFAAGLNNVVT
                     EGNIENSDFRFWGSHFYEWGLTYNTRIAKNNNLLHLKYGMSVMYNNLRPTENRMFVKN
                     GNQTNLEDSGLNLKESRFRNVYLVLPLHLEFDFSGKTIKEDKTYFKTHQSFRMGLGGY
                     AGVRVKSKQIIEYNSNDMEVTQKTKGDFNSSNFIYGLSAYIGYRATSLYVKYDLNPLF
                     KDNAVKQNNVSLGVRFDFN"
     gene            complement(48840..49574)
                     /locus_tag="PK218_01565"
     CDS             complement(48840..49574)
                     /locus_tag="PK218_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97230.1"
                     /translation="MENQIKEKLNAREIQPSTQAWDRLDAMLTVAEEKKTKRFPFWFI
                     GVAASVLVLLTVGLFIFNQEKSISIPNDEIVVTPKNQEPKINDELPITNENQVATTNN
                     PSKIKNQKSAISNQGVSIINQKSNQNSIINRDKEIEYLVAGDVAAVKDIPKVNSTEPI
                     VVQPRKELVQKKSTYVDVDALLASAENSSKVQAKSKLDKIKVDANTLLSHADGEVEQT
                     FREKVINSISKNYKEVKVALSNRNQE"
     gene            complement(49651..50223)
                     /locus_tag="PK218_01570"
     CDS             complement(49651..50223)
                     /locus_tag="PK218_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPW97231.1"
                     /translation="MKVINLHQEEKELIELAVENNRHAQQKIYSKFSPKMLSICRQYI
                     KDIHEAEDIMITAFMKVFTNLKNFKHEGSFEGWIRRIMINECISFIRVQKKVKFLEDE
                     NFTEEKVNNIESQLSIEDIQFLIDSLPDGYKMVFNLYCIEGYKHHEIAAMLGINEGTS
                     KSQLSHARRILQQNVTKLKNYEMSIKKNNQ"
     gene            complement(50379..51356)
                     /locus_tag="PK218_01575"
     CDS             complement(50379..51356)
                     /locus_tag="PK218_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPW97232.1"
                     /translation="MNITSQIKQPIQQEMELFEKKFYESMTSKVALLNRITYYIVNRK
                     GKQMRPMFVFLVAKMVSGGKVNERTYRGASVIELIHTATLVHDDVVDDSNRRRGFFSI
                     NALWKNKIAVLVGDYLLSKGLLLSIDNGDFDLLRIISVAVREMSEGELLQIEKARRLD
                     ITEDVYYEIIRQKTATLIAACCALGAKSVSEDEVLVEKMRKFGELIGMAFQIKDDLFD
                     YTDDAIGKPTGIDIKEQKMTLPLIFALNNCTSKEKSWCINSIKNHNKDKKRVKEVIQF
                     VKDKNGLAFAEQKMIEFQQEALLLLQNFPNSEYKSSLELMVNYVIERKK"
     gene            complement(51471..51543)
                     /locus_tag="PK218_01580"
     tRNA            complement(51471..51543)
                     /locus_tag="PK218_01580"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51508..51510),aa:Gly,seq:tcc)
BASE COUNT        17177 a         8581 c         7919 g        17766 t
ORIGIN      
        1 aaacactgga gcggaagaaa ttctgaaaac atggttacgg tttttccaaa aggagattat
       61 aagccaggag attttgtatt ggtaaaagtt actgattgta ctactgcgac tttgattggc
      121 gaagcggttg ggtattcgga gatgaattag agcttttttt tagccacaaa ttacacaaat
      181 tttcacaaat aaatatcatt ttaaatggat aacaatttgg aatattatta taaaaaagat
      241 gaaaactata caataattgg tctttgtatg gaagttcata gaattttggg tccaggattg
      301 ttggaaattg tgtataaaga tgcgctggaa ttagaattta gaaataataa aattcctttt
      361 gaacgcgaaa aggaatttga aatagaatac aaagggataa agcttccaca taagttttat
      421 gcggatttta tcgtttatga tgatatcatt ttggaaataa aatcagttaa agagattaat
      481 aacgaacata tagcgcaaac attaaattat atgaaacttg cagattctcc aattggaata
      541 attgcaaact ttcaaaacaa atcattagct agtaaacgat tggtgattta atttcaaaaa
      601 ctaatttgtg aaaatttgtg taatttgtgg ctacatttaa aatatggaat cagttcaaag
      661 tataaaacaa cgatttgaga ttattgggaa tgacccaaaa ctaaatcgtg caatagaaaa
      721 agccattcag gttgcgccta ctgatatttc ggtattggtt gtgggtgaaa gcggtgttgg
      781 taaggaaagc attcctagaa ttatccattc gctttcgcac agaaaacacg gaaaatatat
      841 tgcggtaaac tgtggcgcca ttccagaagg aacgattgat agtgaattat ttggtcatga
      901 aaaaggtgct tttaccggtg caacttctac ccgtgaaggt tattttgaag tggctgatgg
      961 tggaactatc tttttagatg aagtaggcga attacctttg actactcagg ttcgtttgtt
     1021 gcgtgttttg gaaaatggcg agtttataaa agtaggttcg tcgcaggttc aaaaaactaa
     1081 tgttcgtatt gtagcggcta ccaacgtgaa tatgtttgac gccattgaaa aaggaaaatt
     1141 tagagaagat ttgtattatc gattgagtac ggttgatatt ccgttgccac cattacgtga
     1201 cagaaaggat gacattcatt tactatttag aaaatttgct gctgattttg ctcataaata
     1261 taaaatgcca gctataaaat tgcgtgaaga tgcgattgct tttttacaga aataccgttg
     1321 gagcggaaac attcgtcagt tgcgaaatgt ggcggaacaa atttcggtat tggaaaccaa
     1381 tagagatatt actttggcga ctttgcaatc gtatttacca acggaaagtt ctaatttacc
     1441 atcagtaatt agtggaaaaa aatcagatag tgattttgct accgaaagag atatattgta
     1501 caaagtgctt tttgacatga aaagtgactt gaatgatttg aagaaactga ctttggaatt
     1561 gattaaaaac ggaacgaaaa ttcaagacat caatccgagt ttaattcaga aagtgtatgg
     1621 ttcaaatgac agtgaaattt cgttcgaaga agaaccacga agaagttcgt tgccaattca
     1681 aagtcctgta attcaagata attatcaaga tgatgaagat caaaatttcc ttgacgctga
     1741 aacggttgaa gaagaagaag ttttaaaatt ggaacaaaaa gaaatagaac taattaaaaa
     1801 atccttggaa cgtcataatg gaaaacgaaa agctgcagcc gatgaattag ggatttctga
     1861 acgaacactt tatagaaaaa taaagcagtt tgatttgtag ttttttgtta catttatact
     1921 cataaaatca aacttatgaa aaatataatt tccttgtttg ttattattat tgttttagtt
     1981 tcttgtaata atgacgattc tactaattca acagtaataa atcctaattt acttcaaaga
     2041 gtagattttt tttcgggaac caattatgaa aaaaggtggt gttttaattc caatggtttt
     2101 ttagaagaaa ttaaagatgc caatgggata ttacttgaaa aatttgttta caattcaaac
     2161 aatcaagtga tacaaaattt gatttatgaa aatggtatcg tttctcaaac cttaaacata
     2221 agctatagtt ctaacaacat tatttctgaa ataaacggaa atccctttaa ttacaattca
     2281 acagaaaata aatattatga ttctgatggg ctattttaca attataactg taatctgaat
     2341 tcagaatttt taatgaaaag ttcttctgct ataaatgatg atcctgacta ctattatgag
     2401 tatacctata atacaattta tatagaaaat aatatgtttt catttagcag ttatgacaca
     2461 aatacatttc aagaaatatt tactttttat gaatttgaca ataatgttaa tcctttaaaa
     2521 caagctctga tgcctgtttt taaagttaaa agtatcacca acccaaaatt ttattatgat
     2581 agtttatctt ctgcaaataa tattatttct caaggttatt tagatgttga tccagaatat
     2641 catacttacg attatacttt taattctaat aatttgcctg taaatcaaac aagaaatgac
     2701 tattatcttg atgtttttga aaattctatc gaatcaaaac attattatta tcaaggcgat
     2761 ataattccaa attaaaaaga attacttttg aaaaattttc ttttaatgaa attcactaaa
     2821 tatattttac tcctttttgt tgtattggtt atcaatagct gttctgtgta taattttact
     2881 ggaactggaa aaattgatgc caaaactttt caagttaatt attttctaaa taatgccgag
     2941 ttgattgaac cgggaattga acgaactttt accttgaggt tgcaagattt aattcaaaat
     3001 cagaccaatt tgaacttgac aaacaccaat ggcgacttgg tttatgaagg tgagattgtt
     3061 cgttataatg ttacaccaat gactgctact gcggatatta gagcagctca aaatagacta
     3121 accattgctg taaatgttcg atttactaat aaaaataagg aagaagataa ttttgagaaa
     3181 caattttcat tttatgatga tttcccagca gatacaccat tagctggctc acaattaaca
     3241 agttcactag atgtaatatt tgaacgaatc actcaagata tttttaatgc atcattagca
     3301 aaatggtagt ttttgaaaaa aatgtatctt taaaatctga aaacaaaaca ctttgaattt
     3361 aactgattat acttatttac ttaacaaacc cgatgctatc aatgaaaagc atgccgaatc
     3421 tttggagaaa gtgatggatg aatttcctta ttttcaaagt gcacgtgtga tgcgattgaa
     3481 gcatttgtat aatcaagata gctacaaata caatcatgct ttaaaggttt cggcagccta
     3541 tactacagat agaagtatgt tgtttgattt tatcacttca gatgattttg ttgcggttca
     3601 aaaaggcttg tatgaaaaga aattacaaga attaatgaac attcatgtag ttgaaagtga
     3661 aattgtagtg gttaatagcg gttataaaag ttcaaatccg ttagaacaat ctattttaaa
     3721 ttctattcaa caatctaatc cagaagaaag tttggcaatt gaaaaacttg aaattgggaa
     3781 accgttagag ttttctaaag atgagaaaca ttcttttgaa gaatggttgc agttggcgcg
     3841 atttagtcca ataataagag aagataaaga agctgacaac gacaaaccta tttcggaaga
     3901 aaaaaagaaa aaaatagatt tgattgataa atttattgag accaatccaa aaattccagc
     3961 aatggacaaa gaaaagtcta ttgctataaa agaaaataga gaagaagaca cttcctatct
     4021 aatgaccgag actttggcta gagtttattt agaacaaaaa aaatatacaa aagcaattca
     4081 agcttatgaa atattaattt tgaaatatcc agaaaaaagt agtttctttg cagaccgaat
     4141 tttagatata aagattttac aacaaaataa taattagtaa tgagtacatt ttcaatcttt
     4201 ttagtattaa tcaccatcgt ttgtttttta ctaatcgtag ttatcatggt tcaaaaccca
     4261 aaaggtggcg gactttcttc atcctttggt ggatcagcac aaatgggtgg tgttcaaaaa
     4321 acttcagact ttttagataa aagtacttgg acattagcaa cagtcttaat cgtattaata
     4381 ttgttatcaa gtttaagttt cagtggtgct ttatccgatt cgagttcaaa aatcattgat
     4441 gaaaccgaaa aagcagctcc tgcaaagcca gcagatacaa aaacagttgc acctgcaact
     4501 tctactactc cagcttcacc tgctgaagga caaaaataat ctagagttat aaaatacata
     4561 atgccagcat gtcagtgttg gcattttttt tggtaaaaag tgtcagtttt aaaggcttgg
     4621 cacaatttct gaaaaaacaa aaacaactaa taaattttta atttaattaa atataaaaac
     4681 tatggcatta aacattaaac cactttcaga ccgagttatt gtggaaccag cagcagctga
     4741 aacacaaact gcttcaggaa tcataattcc agatacagca aaagaaaaac cacaaaaagg
     4801 aactgtagtt gcggcaggaa aaggtaaaaa agatgagcca atgaccgtaa aaattggtga
     4861 tactgttctt tatggaaaat atgctggaac ggaattaaag ttcgatggaa aagattatct
     4921 tatcatgaga gaagatgata ttttagcaat aatctaatta gtagtaagat tttgagaatt
     4981 aagtattaag actgaaattt aaacaaacaa caaaatggca aaagacataa aatttgatat
     5041 tgaagcacgt gatggattaa aacgtggcgt tgacgcatta gcaaatgcag taaaagttac
     5101 cttaggacca aaaggtagaa atgtaattat ttcaaaatca tttggaggac caacagtaac
     5161 aaaagatggt gtttctgttg caaaagaaat agaattaaaa gatccattgg aaaatatggg
     5221 tgcgcaaatg gtaaaagaag ttgcatcaaa aaccaacgat ttagctggtg atggaacaac
     5281 aactgctacc gttttggcac aagcaatagt aaaagaaggt ttaaagaacg ttgctgctgg
     5341 tgcaaaccca atggatttaa aaagaggaat tgacaaagcc gttgaagcta ttgttgctga
     5401 cttaggaaaa caagccaaag aagttggaag ttcttcaaat aaaattaaac aagtagcatc
     5461 aatttctgca aataatgacg aagttattgg tgatttaatt gctactgctt ttggaaaagt
     5521 aggcaaagaa ggagtaatta ctgttgaaga agcaaaagga actgatactt atgttgatgt
     5581 tgtggaagga atgcaatttg accgtggtta tttatctcct tattttgtta ccaatccaga
     5641 aaaaatgaat gtagaattgg aaaatccata cattctttta tacgataaaa aagtatcttc
     5701 tttaaaagaa ttactaccag ttttagaacc tgttgcacaa tcaggaaaac cgttagtgat
     5761 tattgcggaa gatgttgatg gtgaagcttt atcaacatta gtagtaaaca aattacgtgg
     5821 tgcattaaaa attgctgcgg ttaaagctcc aggttttgga gatagaagaa aagcgatgtt
     5881 agaagatatt gcaatcttga ctggcggaac agttatttcg gaagaaagcg gttatacctt
     5941 agaaaatgct actttagaaa tgcttggaac tgcggaaaaa gtgactattg acaaagacaa
     6001 tactacttta gtaaatggtg ctggaagtgc agatttaatc aaaaatagag taaatcaaat
     6061 caaagcacaa atggaaacca ctacatctga ctatgataga gaaaaattgc aagaacgttt
     6121 agctaaattg gctggcggtg ttgctgttct ttatgttggt gcagcctctg aagttgaaat
     6181 gaaagagaaa aaagacagag ttgatgatgc attacatgct acacgtgctg ctgttgaaga
     6241 aggcattgtc gcaggtggtg gcgttgcttt attgagagct aagaaagcat tagacaaagt
     6301 aaaagcggaa aatgctgatg aagcgactgg aattcaaatt gtatcaaaag ctattgaaag
     6361 ccctctaaga actatagttg aaaatgcagg acttgaaggt tcggttgtgg ttgcaaaagt
     6421 atctgaagga aaagataact atggttataa tgccaaaaca gataaatatg ttgacatgct
     6481 aaaagctgga attattgatc ctaaaaaagt aacccgcgtt gctttagaaa atgcagcttc
     6541 tgtttctgga atgatactta ctacagaatg tgctttaatt gatattaaag atgaaaacgc
     6601 tggtggagga atgccaatgg gcggaggaat gccaggaatg atgtaatgct ataaattcta
     6661 aaaaattaaa taacaaattc caaaaatcgc ctcaattgag gcgatttttt atgctttaga
     6721 tttctctttt ttaaacattt taagaattac gggaactgta gaaattaaaa tgataatgat
     6781 aatgattttc tcaatatgat gttttaaatc aatattaaat tccgttaaga aaagcccata
     6841 caaataatgt cctgaaaata ttaatgtgaa tgaccaaaga aaagaactta atatgttata
     6901 gaacataaat ttctttttgt ccataaaaac aatacctgca actattggcg caaaagttct
     6961 gaaaacaggt aaaaaacgag caaaaattat agcttttcct ccatatctct cgaagaaatc
     7021 ctttgattga agaagatatt ttttcttaaa ccaaaaacta tcttcttttt tataaagata
     7081 ataaccgctt ttggaaccaa accaataccc tatcatgtta ccaaaaatac ctgatatggc
     7141 cactaataaa gctagaaaaa aaacatttac aaaatcactc tctattatgg tgaagttttc
     7201 tactagactt ctactgtaga ttccagctaa gaaaagcaaa ctatcacctg gcaaaaagaa
     7261 tccagcaaac aaaccagttt cggcaaaaac aataaataaa acgatccata atccaacctg
     7321 aataccacca aatgtcattg tgatataaaa ctctggattc aacaactgca tccaatcaaa
     7381 attattcatg ttttagcttt tttaattcga gcgtgaattt aacattattt ttttagaaat
     7441 cttgaataaa aaaaaacgtt aactaatctt taggaaattt taaaaatcaa gaagcgttaa
     7501 tgtcagttat tctcgttcat acatgcaaca accatgaagt ttttgatatt gttcttcggt
     7561 ggctttaatc tcatctgtat catgaccgac ttctgcaatt gctttcttta catcaattat
     7621 agagcatttt ttttcgttaa taatcaaatg caaaacatga tcgtcaatat gccattcggc
     7681 actttttact cctgaaactg aaaaagcagc attttcaatt ctttttttac acatttcaca
     7741 attgccatta actgttatat catatttggc atttttattt tgtttctctt gtgcttgtat
     7801 tgaaaatgaa actaaaaaaa gcatcattcc tattattata tttttcataa ttgtagttgt
     7861 ttattgtttg taatctattt tattttaaat cgcaatcctg cataatacat atttccaaaa
     7921 ataggcgcat agactattga agcatcaaaa tttgttccaa aaggattatc gctgtcgaga
     7981 atagctctat cttgtctgta atttccaata ttttctccgc ccaaatacat ttcaaaaata
     8041 ctcgaaaatg ttcgagtaac ttgaaaattc attacagcaa atgaaggcga aaactctgga
     8101 aatctatctt cttcaggatt agaagcagta taaggtaatt gttgttttcc taaccaattg
     8161 gttgtaaaat cgaacttcca ttgttgtcct ttatccaaaa tatgagtttc gtattcaata
     8221 ttggcaaaca atctatgttt ggcttgcaat ggtcgttgaa aagttccaga aatataatcg
     8281 gttgcaatgt cataatactt ataagcagtt cttaaattca agtgtttttt taattctata
     8341 ttaaaatcaa attgcaaact attggcaaat gactttcctt ttaaattgta aaacaaaact
     8401 tgttgaggag aattcattac atctactact gcttgatttt gaaaatcagt tctataaaaa
     8461 tcaagagtta tgtctgcact ttttgagaaa aatttgaatt tttgagtaaa actaacacca
     8521 taattccatg ctatttcagg atttaaacca tagatttttc cattagtatc taaaactgaa
     8581 aactgccttg aactagcaaa aagtgtttga ttttccgcaa aaatattggc agctcgtttt
     8641 cctcttccag cagaaagtcg caatacactg ttttcaaaaa cattatattt tacatgcaat
     8701 cgcggtgtga aaaaagttcc taatcgatta tgagtatcaa ttcgtccgcc aagaatataa
     8761 ctaaatttat cactatcatc aaaagtatat tcaaaaaatg caccaataga attatctatt
     8821 ctgcttacat caattgtgtt tacgaactcc gaataattat caaatgcaaa atttattccg
     8881 gcagaaaatt tattacgagt attgtcaata attgaattaa aaatcaggtt agaataaaaa
     8941 ctttgttgtt tgatatcata ctgattcaaa ccatagtaag actgttgatt atgcaacgaa
     9001 aacgcatttt gaaaaccgat gctttgatat ggcatatctt taaaaacata tccaatttta
     9061 gaagaaaaat caagccgttc ggtttcaatc tcagaacccc aatgattcgt tgttaattta
     9121 tctctattcg ggtcaaaatg tatttcgccc gatagttttt catcacgcat aaaccgagca
     9181 ttcaagaaag aaacccagcc cgtttcttga ttggcatatt gccatcgatt ggttacattc
     9241 aattgttttc cttgaggatt atcaagaaaa ccatcattat tgctgtcatg atgaccaaag
     9301 cgaccactac cgtgaactag caaactcgtt gcccatttat cgtttacttt tttattgaaa
     9361 tgcgtgttta actcaaatcg gctactgtta tcgccataag catttagaaa aaaaagaata
     9421 tcatctattg gttttaataa ttcggtatta atttgtccag aaatgctttc ataaccgttg
     9481 acaacacttc cagcgccttt agttatttga atactttcca cccaagttcc tggtgtaaaa
     9541 gacatgccat aagcttgtga tgcgccacga acagaaggaa tattttcttc agtaatcata
     9601 atatatgggc ttgttaaacc taacattttg atttgctttg ttccagttaa tgcatccgaa
     9661 aaattaacgt caattgatgg atttgtttcg aagctttcgg ccaaattaca acatgctgct
     9721 tttagcaatt ctttgctagt cacaagagtt gtatttgaag ctccttttag agattttttt
     9781 aatccttttt gttgtacttt tatttttact tcttctaatt ctttctgaga aaatgaattg
     9841 gtaaaaaata acaacgcaaa gaatagaatt gtttttttca ttagtgtaat tttaatttat
     9901 ttatattcta aaaagattct ataaaaaaat taaaattatg agtagaatgt atattgacag
     9961 tagagtttgt ataatggcgg cgcattagcg tcacaactat aaacaatttt ttctttagct
    10021 tttttcgaaa aaacttgatt aaattctaaa ggtgtaaatt cattaaagac aataacaaaa
    10081 tttgcatcaa atgaaatttt tattaaaact ttttctacgt cgtcttttag ggttacttct
    10141 ttatccgaac aacatttgct atgtgtcgtt tcaacttttg cacaacatgt tttttcaaca
    10201 acaattggca tatcacaaac ttcttctttt gagaaaactg aagttataga agctatcttt
    10261 ccttcacaaa aatgcacatt aaaagccaat ccagaattgg aaaccaatac cagaaatgct
    10321 attaaaatac ttagttgttt tttgaaaatc atagcgcaaa gatactataa gttgattttt
    10381 aagttcttaa tttataatta atttttcaaa tttctatttc ttgtcccatt aatggaatag
    10441 aacattcaat gccaagtttt tcttttattt ttatgcgaag ttcatctgct ggttgatttt
    10501 caccgtgaac aagaaatact ttttgcggct tagtttctaa atctgacaac caatttatta
    10561 aatcattttg gtcgccatgt gccgaaagac cttctacttc taaaattttt gcattcacag
    10621 aataatattt tccataaatc ttaatttcat ttgcaccttc caataatttt ctgcctcgcg
    10681 ttccttctgc ttgataacca acaataatta cagaagtttc aggttttcca atatattctt
    10741 ccaagtaact caaaattctt ccaccagtaa tcattccgct tgcagctatt acaacttttg
    10801 gttttggatt ataaattgtg tcgatggttt ctttgtagtc agaaattaaa gtgaaaattt
    10861 tacacatttc ttcacattca tgagcattta acttgtgcca agaagtgttt ttttgaaaaa
    10921 catccaatac atttattccc attggcgtat caattatcaa tggaatatca ggcagttttt
    10981 gttctttttt tagttgccat aacaaaaaca tcaatgtttg tactctttca acagcaaaac
    11041 tcggaattaa aactgttccg tgattatgat atgtgttatt gataaacatt tctaacagta
    11101 gttttgtgtc ttctgttgga tgaactctat taccataagt gctttctaaa aatacataat
    11161 ctgctttttt aggttttgtt ggcggataca tcaatacatc attatcccta ccaatatcgc
    11221 ctgaaaaaac taaaattttg ttctcaattt taagctctat ggaacatgcg ccaataatat
    11281 gaccagcatt tttgaaaact gcagaaattt cagcatctag atttatttct tcattttctt
    11341 taataattct aaaatgagga aaaacttttt ctgcttgact tacatcgtac aaaggttcag
    11401 ctaaatcatg ttttgaatac tttcctttat ttgccttttc agcttcttct tcctgaattt
    11461 ttgcactatc taataaaatt aattttgaaa ttgcagctgt tggacttgta caaaatattt
    11521 ttccagaaaa cccttgattt actaaccttg gcaaccaacc gcaatgatca agatgaccat
    11581 gagttaacaa aacaaaatca atagttgaag gtaaaaccgg caatggttcc caattgagtt
    11641 cgcgcaaagg ttttattccc tgaaacaaac cacaatctat tagaattctg attccattac
    11701 tttcaattaa tgttttcgaa ccagtaactg ttcctgctcc gccaataaat tttattttca
    11761 ttttatatat aattacaaag ttctgttgct tcttttaaaa tatttacact acgcttttga
    11821 ctgattccaa ttttactcaa caaatcaggc gtttttatta aatgtgaaac taagattaaa
    11881 cccaattcta ataatttact tttttcagca gaagaaatgg ttgtcaaaca cgttattgga
    11941 tacaaaccat tacgatcaac tttttttctt aaacagtttt cagcaggata atcccaactt
    12001 aacaattgta atcctgagca agtcccaaat gcaatagcat ccgatgtaaa cctgttgttt
    12061 gtaactatta tacattttga aacctcatca ttattagaaa aaatggaatg cttttgactt
    12121 tttaaatcat taaacctcga caaaatatac atgggaactt taacatctgt gactttatcg
    12181 tttgtattat gaaatttgca ttcaataatt gaaatcagct catccttttt aatcacaacg
    12241 tcaatttcgt gagaaataca ttttccattc aggaaaaggt ttgtttttga tgaataacct
    12301 tctgaagaaa ataattctgc aataaatttt tcgaaataaa aacctgctgg tccaagctgt
    12361 tgaatggccg ttctcaaatt atacctagca gcatgagatt ttgcagattt ttttaataat
    12421 gaaaaggcaa gtttataaat ttttcttgtt gaaattcctt cataaagttg gctttctatt
    12481 tgtgccaata ctttctcaac agtttcaata cttgcgccag attttaagag cgaatgtttt
    12541 aatttttgaa tattaaaatc gacaacttga ccagattgtt ttataatttt catcctcaat
    12601 atttatctat taaagataca aaaattaatc ttatttagct attttttgat aataaaaccc
    12661 agggataaat agtaaaataa aaataggttg aattaaaaat cttctaatgt agaacaacgt
    12721 cattttgctt tcttcggcaa aaaatgtgag cacaaaaaag aaacttatta atagaataga
    12781 gcctagaagc ataaataaga aaatagaaaa tttcactatt tttcggtcat caaaaagcac
    12841 atagagcaat cctaaagaaa taaccatatt caaataaaac cgaaaaccta agctgaaaaa
    12901 aagtttaaaa atattgaatt ttggaaaagg caaattggca tattcactac taaaataatt
    12961 caaaaaaggg tcgtaaaaca attggttttc aaacattcga atcaatacta acactaaaat
    13021 taatagtgtc gaatagatta cttttgattt atattttttt agaaacgcta gcataagacg
    13081 aatagttatt aacccaaatt acccacaaaa caaaaacaac accatatata atcaacggaa
    13141 aaagtacacc atgtaaaaaa tgctcaaatt gaggatagaa atacaatgcc atactcaaca
    13201 aagcaattcg agaaacattt aaaacatgaa taataacaat tccgaaaagc aggaacagta
    13261 aagtgttttt aaattttccg ctaaatgcaa ttacaaaagc aacaaataaa atcatcacac
    13321 tcaaaccatt acaaccttca ataactctgg agatatactt gtttttatag aacaacttta
    13381 tacttggctc tgtcaaatgt ttcgttgtat aggaatgatt gtcaattaat gacaaaaccc
    13441 attccgtttg ttcggcaaca ctttttgtaa aaccatctac ttcggcattt ttaacatcaa
    13501 attgattgag atagctttga tacagaattg ttaagattaa gtaacttaaa aaaaacttac
    13561 ccaaaaaaag caaaaaaggt ttgtattgtt gcagcagatt tttcaaagtt gaatttttaa
    13621 caaatttata taaaataaac ccttttcgtt aaatactttt gtataaaaat ttgaaaaatg
    13681 gaattagacg atttaaaacc agaagtagca actatattga atgacaacaa aattgaccga
    13741 gatacaaagt tgaaaaatct ttgtcaattt ttatccgatt ctgtttccta ctataattgg
    13801 gttgggtttt attttgccaa tcacgaaact aaaacattac atcttgggcc ttatgttggt
    13861 gccgaaaccg atcatactgt tattccgttt gggaaaggaa tttgtggaca agttgccgag
    13921 tcgaatgcta attttgtagt gccagatgtt gctgctcaag acaattatat agcgtgcagt
    13981 tttacagtga aatcggaaat tgtagtgcct ctttttgttc acggaaaaaa cattggtcaa
    14041 atagacattg atagtcacgt aattgaccct tttacagaaa ctgatgaacg ttttttagaa
    14101 tttgtaaata aagaagtagc aaagttattt tagggtattg aactattgta attatcgttt
    14161 ttcttccgaa tttcatcaaa tgacagcctt atgaaactaa acataaacaa caaatttacg
    14221 aacgaacttc cggcagacgc tttggattca aacgaaacca gacaagtggc tggagctgca
    14281 ttttcttatg taaatcctgt aaaacccaaa aaaccttgtt taattcatgt ttcgaaggaa
    14341 cttgccgaaa gtgttggaat ttcgttagaa gaaataaaat ctgatgcgtt tttaactatt
    14401 ttttcaggaa aaaccatttt agaaaagaca aaaccttatg ccatggctta tgctggacat
    14461 caatttggaa attgggctgg gcaattaggc gacggaagag ctattacact ttttgaagca
    14521 attcataata atagaagcac aatgtttcaa ttaaaaggtg ctggaaaaac tccttattct
    14581 agaagtggtg atggtttagc agtgttacgc tcctccattc gagaacattt gtgtagtgaa
    14641 gccatgtttc atttaggcgt tccaaccact cgttctttag cattatttac tagtggcgac
    14701 atggttttac gtgatatttt atacaatgga aatccagctt atgaaaaagg tgccatagtt
    14761 tgtagaacag catcaagttt tattcgtttt ggcaattttg aattatttgc ttctcaaaat
    14821 gacatcaaaa accttcaact actaaccgat tatacaataa aacattattt ttctgaaata
    14881 aattcagtag gaaaagagaa ataccttgaa ttctttgaaa ccgtagctga taaaactcgt
    14941 gaaatgattg tgcattggca acgggttgga tttgtacatg gtgtaatgaa taccgataac
    15001 atgtcaatac atggaataac aattgattat gggccttatg gttggctgga agactttaac
    15061 ccgaattgga cgccaaacac tactgataat caatataaac gataccgatt cggtaatcaa
    15121 ccgcaaattg cacaatggaa tttactgcaa ttagcgaatg ctttattccc tttgatagaa
    15181 gaggcgaagc ctttagaaaa aattttgaat gactttccaa taaaatatga agcggattat
    15241 attgaaatga tgcacagcaa attaggcttt gagttttcaa gtgaagcatc taaaaacatc
    15301 atcaatcttt tgactcaaaa tcttcaatta acagaaactg atatgacaat tttctttaga
    15361 agtttaagtc tagtttccaa agttgattcc gttgaaaatg cttttgaaaa aattgcaact
    15421 tcattttata aattagaaga agtaaaagac tccgtttatg acacttggat gtattggttt
    15481 actttgtata ttaatcaact aaaattagaa aatcaatctg attctgaaag aaaaaaaacc
    15541 atgaatttgg ttaacccaaa atacgtttta cgcaattata tggcacaact tgccattgat
    15601 gctgctgata aagaagatta taatttggtt aacgaattgt atgaaatgct tttaaaacct
    15661 tataacgaac aaaaacaata tgaaaaatgg tttgcaaaaa gacccgattg ggcaagatat
    15721 aaagtaggct gctcggcttt atcatgtagc tcataaataa aaaagacagc tctacaattt
    15781 ctaataatca tttagaatac tatttctttg aaaaataata ctcaattttg aaaaaacttc
    15841 tctgtgaaat aaaaaagtgc aaagtttgtg aaccttattt accattgggt gcaaatcctg
    15901 ttttgtatgt aagtaagacc agtaaaatca taattattgg tcaagcacct ggaaaaattg
    15961 tgcataatac tggcattgct tggaatgata aaagtggtga tactttaaga aaatggctgg
    16021 gtgttgataa agaaacattt tatgatgata aacaatttgc aacagttcca atgggttttt
    16081 gttatccagg aactggaaaa aatggtgatt ttccaccaag aaaagaatgt ttcccattgt
    16141 ggcatcataa aattttatcc gaaatagaag gaaatccact gattttgttg attggacaat
    16201 atgcacaaaa aaaatattta aacaaaaaag gaaaatcaaa cttaacagaa accgtaaaat
    16261 cattccatga ttacttgcca aattattttc cattgcctca tccatcacca agaaataata
    16321 tttggatgaa gaaaaatttt tggtttgaaa aagacgtttt acctgaatta aaaaaaagaa
    16381 tcgctggatt aataaaaaac aactgaaaaa aatattagtc aaatttaata acttacctac
    16441 tttctgttta ctgctgattt ttacaacgtt ttgattataa attcaaatac taaagaagga
    16501 ttttaaaaat tttcacaaaa aagaagttat aaaattattt taatctcatt attttatttg
    16561 taaaatcaga aaagaagatt acatttgcaa ccgaattgag aaaacctcat ttcatacata
    16621 ataatcattt ataacaatat ttgcatgtac ttaagtaaag aaacaaaagc agaaatcttc
    16681 gctaaacacg gaggaaaagc agaaaacaca ggatctgcag aaggacaaat cgcattgttc
    16741 acatttagaa tctcacattt aactgagcac ttgaaaaaaa atcgtcacga ttataatacg
    16801 gagcgttcgt tagttctttt ggtaggtaaa agaagaagtc ttcttgacta tttaaagaaa
    16861 aaagatataa caagatatcg tgaaattatt aaagaattga atattagaaa ataatcaata
    16921 taaaagaggt gcgaaagtgc ctctttttta ttttagattt taaatttaaa aaaagtttgg
    16981 tttttcattg gaacacaaca actacaacaa cacaactatt ctgtcgacag gcaggcaaac
    17041 caatgtaaaa ttaaaaagga aaatttatga ttccacaatt atttgtagaa agcatcgatt
    17101 taggtgatgg cagaaacatc acaatcgaga caggtcgttt ggctaaacaa gcagacggat
    17161 ctgtagtagt aagaattggt aaaactgtaa ttttaggaac agttgtatca gcaagaaaag
    17221 caagtccagg tattgatttt ttaccattaa cggtagatta tcgtgaaaaa tttgcagcag
    17281 caggtcgttt tcctggaggt ttcttcaaaa gagaagctcg tccaagtgat agcgaagtat
    17341 taacaatgag gttagttgac cgtgttttgc gtccactttt cccaagtgat tatcatgcag
    17401 aagttcaagt aatgattcag ttgatgtctc atgacgaaga agttatgcca gatgcattag
    17461 caggattagc tgcatcagct gcattagcat tatctgacat tccgtttgaa actttgattt
    17521 cggaagccag agtaggaagg attgacggaa aatttatcat caatccatca cgtgcgcaat
    17581 tagaattatc agacattgat atgatgattg gtgcttcaag agattctatc gcaatggtag
    17641 aaggagaaat gaaagaaatt tcagaaaaag aaatggtaga agccattaaa tttgcacacg
    17701 aacatattaa agtacaaatt gacgctcaag aaagattagc agcagctttt ggcaaaaaag
    17761 aagttcgtac ttatgaaggc gaaagagaag atgaagcaat ttacgctaaa gtaaaagctg
    17821 catcttatga taaaatttat gccattgcaa gtaaaggttc tgcaaaacac gaacgttcag
    17881 ctgcatttga cgaagtaaaa gaagaagtaa aagccttatt tacagaagaa gaattggcag
    17941 aaaatggcga tttagttgga aaatatttct acaaagtcaa caaagaagca gtacgtaatg
    18001 taactttaga tttaggaact cgtttagacg gtagaaaaac tacagaaatc agaccaattt
    18061 ggtgtgaagt agattattta ccatcagttc acggttcggc attgtttaca cgtggagaaa
    18121 ctcaagcttt ggctacagca actttaggaa cctcaagaga agctaaccaa attgactcgc
    18181 ctagcgaaca aggagaagaa aaattctatt tacactacaa cttcccacct ttctcaactg
    18241 gtgaagctcg tcctttaaga ggaacttctc gtcgtgaagt tggacacgga aatttggcac
    18301 aacgtgcttt aaaaaacatg attcctgctg attgtcctta taccattcgt gttgtttctg
    18361 aagttttaga atcgaatggt tcatcttcaa tggcgaccgt ttgtgctgga acattatcct
    18421 taatggatgc tggagttcaa atgattcgtc cagtttctgg aattgcaatg ggattaatta
    18481 cggatggcga tcgttttgcg gttttatctg atattttagg tgatgaagat catttaggag
    18541 atatggattt caaagttact ggaacttctg aaggaataac tgcttgtcaa atggacatca
    18601 aaattgacgg attgaaatat gaaatcatgg agcaagcttt agctcaagct cgtgatggac
    18661 gtttacatat attaggaaaa ttaacggaaa cattagcaca accaagagct gaagttaaag
    18721 atcattcccc aaaaatcatt acaagaagaa ttcctaatgc gtttattgga gcattaattg
    18781 gacctggtgg aaaagtaatt caagaattac aaaaagctac tggaacaaca attgttatca
    18841 atgaagatcc agttacagaa gaaggaattg ttgaaatttt aggaactgac ccagctggaa
    18901 ttgctactgt tatggctaaa attgactcta tcattttcaa accaactgtt ggtgaagcat
    18961 atgaagtaaa agtaattaaa atgttagatt ttggagctgt tgtagaatat acgcaagcac
    19021 ctggaaacga agttttatta cacgtatcag aattagcttg ggaaagaact gaaaatgtta
    19081 ctgatgttgt aaatatgggt gatgtcttca tggtcaaata ctttggttta gatccgaaaa
    19141 ctagaaaaga aaaagtgtcg agaaaagcac ttttgccaag acctccaaga gaagaaaata
    19201 aaattactcc aaaaggagaa taaccatttt tttaattaaa gctaaaaccc caatttaaaa
    19261 tttaaattgg ggtttagttt ttttttacta tattatctct aacaaaactc aaaaatttaa
    19321 aagacataag catgaaacta aacattgaaa taaaatccat ttatacctta gatgaaattc
    19381 caaattattg gacaaatcaa gactatgtag ctttactagc tgcttttggg tttgcagatg
    19441 gaaatacaat taagcccgaa aatttaactg aaatgttatt tatggcaatt gccgattatg
    19501 aacctaatga agcagctcaa attgttctta cttacaaatt atcgaatgat ttaaacgaag
    19561 gtcaaattga ccaactatca aacgagatgt tacttgataa agtatgcgaa gaatatcctg
    19621 aaattcattt acacaaacat ttattttcca ttaatcaatt gctttacaaa gcatttaacg
    19681 gaaaatttcc aaatgcaaaa gccagttcta ttcaattttc tgttttagga aatgattcaa
    19741 atgaattgtc aaaatcggat attttaaaag ctttcaatag tggattgaac gaaagcaaca
    19801 ttattaaaag attatttggt gaaccaatga ccacaggtgc taaatttcct gaagccgaaa
    19861 gtctcatttg ggaattaaat actaaagagg attcaacctt tcaattaatc acttcggaat
    19921 atttaataga tagagagtct tttataaata tggaatttga tggtgaaatt ttgaatgaag
    19981 aagaataagt tgaaataaat caatttgtta taaattaaaa aaattgtggt tatctttacc
    20041 gctttcatat tttaaaatta aaatgagttc acacaaaaaa cttaaagaat ttgcctcaac
    20101 agccatttgt ggaaacgaca ttacatcgtc atgtttatat gtttcagctt tagccataat
    20161 gtatgctggg caatatgcct ggatttcgtt attaatcgtt gctggagttt tatttttatt
    20221 ccgaaaaatt tatggtgagg ctgttggcgc attaccttta aatggtggtg cttataatat
    20281 cttgttaaac accacttcaa aaagcaatgc ctctatcgcc gcttgtctaa cgatactctc
    20341 atatatggct acggcggttc tatctgccag cgaagctatg cattatcttc acagcttagt
    20401 gtcttctgta cctgtatttt gggctacaat tggattgcta acattcttct tagtactaat
    20461 tatgtctgga ataaaagaat cctcgaatgt ggctattttc atttttgttt ttcatttggc
    20521 atcgatgatt tttctaattg gaagcggtat ttggtttgcc tattcgaaca attttgacat
    20581 catgtcaatt aatttcagtc aaccaataga aggaagcatt cctgttgctt tgtttttagg
    20641 atttagtact gctatgcttg gaatttctgg ttttgaaagt tcggcaaact ttgtagaaga
    20701 gcaagaacct ggcgttttca gaaaaacact tcgtaatatg tggatagccg taagtgtaat
    20761 caatccatta atggcattgg taattattgg cgttgtgcct ctgattgaag tgggtgcaca
    20821 caaagaaacg ctactttctc acttaggaaa tttaactggt ggatcaaact tggctacctt
    20881 aatttcaatt gatgcagttt tagtattaag tggtgctgtt ttaacttctt atgttggagt
    20941 atcgggttta atgaaacgta tgtcattaga tagaatttta ccccaagcat tgttaaaaga
    21001 aaacaaatca ggaagttcac caagaatttt gattgtcttt ttcttattgt gtgtctctgt
    21061 tttatttatc accaatggtg aattaggacc attggcaggg gtttatacca tgtcattttt
    21121 agcggttatg gcttattttg gatttggaaa ctttctacta aaaataaaac gaacaaaact
    21181 gccaagaccc gaatacgcac aaccttatgc ggtagccatt gctattttag ccattataat
    21241 tgctatttat ggaaacatac tcatgcatcc tgaatattta gtggtgtttc tacagtattt
    21301 tgttccgtcc attgtattga tattgttatt actaaatcga aaatttgtat tgcagtatct
    21361 actagtggtt ataaaaagtt ttattgattc ttttagaaaa cacgctagaa ttggagaagt
    21421 attattaact aaaaaaataa atcgtttaac ccaacaagaa tttgtgtatt tttctaaagg
    21481 agataatatt tcgcaattga acaaagcgat attatatgtt caagaaaatg aaattacaaa
    21541 aaaattaaaa attgttacaa ttgtaaatga agacaagcaa gtgacggatg aatttatgag
    21601 cgattttaat gcattagaca gagcttatcc tgaaataaaa ttagaataca ttcaaatgga
    21661 aggtgagttt ggaccaaaac taattgagac attgagtgaa aaatggaaaa taccatctaa
    21721 tttcatgttt ataagttcgc caggaaatcg tttttcttat agggtttccg agttaaatgg
    21781 tgttcgatta attatgtaga aaaaaaaatt acatctaaca ttaataaatt gtaacttttt
    21841 tgaatctaaa tgcgtataac catctgaaca ctttaaaaga aaaaggaaaa attcaatgag
    21901 acaactcaaa atcaccaagc aggtaacgaa tcgtgaaact gcttccttag acaaatattt
    21961 acaagaaatc ggaaaagttg atttgattac tgctgatgaa gaagtagaat tagctcaaaa
    22021 aatcaaagct ggtgatcaac gtgctttaga aaaattaaca aaagctaatt tgcgtttcgt
    22081 agtttctgta gcaaaacaat accaaaatca aggtttaact ttgcctgatt taatcaacga
    22141 aggaaatctt gggctgatta aagcagcgca acgttttgat gaaactcgtg gttttaaatt
    22201 tatctcttat gccgtttggt ggattcgtca atcgattttg caagccttgg ctgaacaatc
    22261 tcgtatcgtc cgtctgccat taaacaagat tggctccatt aataaaataa acaaaatgta
    22321 cgctttatta gagcaatcaa acgagcgtcc accaagtgct gaagaaattg ccaaagaatt
    22381 ggacatgact gtaaatgatg taaaagaatc tatgaaaaat tctggccgtc acttatccat
    22441 ggatgcacca cttgttgaag gagaagattc taatctttac gatgttttac gttctggcga
    22501 atctccaaat cctgacagag agttaattca cgaatcactt cgtaccgaaa ttgagcgctc
    22561 tttagaaaca ttgacaccaa gagaagcaga cgtagttcgt ttgtattttg gtttaggcga
    22621 tcaacatcca atgacgttag aagaaattgg ggaaactttc gacttaactc gcgagcgtgt
    22681 tcgtcaaatt aaagaaaaag caatccgtag attaaaacat acttctagaa gtaaaatatt
    22741 aaaaacttac ttggggtaag tttaaaattt gaataaaaaa aattccaaat cacattgttg
    22801 gaatttaact ttaactgaca gcctgattaa ctgttcgttt tttgattgat gattgaaact
    22861 ccggctgatt accggagttt taccttttta atacgttaag cacaactttt aaacccttaa
    22921 tcttttaaac ttttaaacta aaaactatct ttgtataaac tactcaactc atgaaaaaca
    22981 ctttaattgc accatctgtt ctagcagctg attttgcaaa tcttcaacgt gatatcgaat
    23041 taattaacac tagcgaagcc gattggtttc atatagatat tatggatggt gtctttgttc
    23101 caaacatctc ttttggaatg cccgttttgc aagctatttc aaaacatgca aaaaaaacaa
    23161 tagatgttca tttaatgatt attgacccag atagatatat taaaactttc gcacaacttg
    23221 ggtcaaatat cctaacagtt cattttgaag cttgtacaca tttgcacaga acattgcagg
    23281 ccataaaagc agaaggtatg aaagctggtg ttgctctaaa tccacacacc aatgttgatt
    23341 tactagaaga tgtcattcaa gatattgatt tggtttgtgt tatgagtgtg aatcctggat
    23401 ttggtgggca aagttttatt gaaaacacct attcaaaagt ggagaaactt aaagccttaa
    23461 ttcaaagaaa aaatgcttca acattaatag aaattgatgg tggtgtaacc tctaaaaacg
    23521 ccaaacaatt agttgatgct ggtgctgatg ttttagtagc cggaagtttt gtgtttactt
    23581 cggaaaatcc aatacaaacg attagcgatt tgaagaaagt tgcttctaat taatgtttgt
    23641 gattcaacat ctttgaaaca ttggcatata attctggaaa ttcatttttg aactgctgtg
    23701 gcgtttcaaa ataatgctca atgattacag aaagaaattc aaattgattg gtgtaagcat
    23761 aaattctaaa ataatttgaa tcaactaatc gttgtctatt gggcggatga tttacttctt
    23821 taatgatggt ttcgaaatgc tttttgaaag acaaagaact gccatcatta tttcttaaac
    23881 tgtggtgatg aataacatgg ctaaattcgt gaataccaag atttaaatta tcattactaa
    23941 tctgacaacc cgcttcaaaa tgtttccaag aaaatgccac tgctttcatt cttggattga
    24001 attcaccctt gtgataatct tcagtttgag ttgaaaaata ttcttccgga tatataatta
    24061 ttttatcaaa aacatctact ataaattttc gcattccaaa agttaacatc acataagtag
    24121 cactaattaa aatttgtgct tctttgttca tttgaaaatc tcctctggct ataaattgat
    24181 agtttttttg aaaaactgca attctatgat ggaaatattt tttatgcttt tctgaaagtt
    24241 tattgtaaaa atcaaaattc tgaattaata aaaaccgttc tttttcagaa attttctttg
    24301 gcattaaata caaatgaaca tacaaaggac gatcaaaagt tgaaatccaa atatcatcta
    24361 aaagacttaa aacaaacatt atggaatata gcaaaaaagc tccaattact aaaattccga
    24421 acagcaagag aataattatt ccaactattt ccatttttaa aagtaactaa attaataaat
    24481 agtggaatta ttttgtgacc gcgaagggat tcgaaccccc aaccctcaga gccgaaatct
    24541 gatattctat ccagttgaac tacgcagcct gtttgaagta tgaggcacaa aatacgaagt
    24601 acgagcaaaa actttgcact tcgtattttg tgcctcgtat ttcatattat gttaatagtt
    24661 ttttcactat tgtcgaaata acttttcctt ctgcttgacc tccaatttga gtcgatgctg
    24721 ctcccattac ttttcccatt gctgcaatac cagaagcacc agtttcggtg attattttag
    24781 aaataattgc ttcgatttct gcttcactca tttgtgcagg aaggaatttt tcgataactg
    24841 caatttgtgc taactctggt tctgcaagat cagaacggtt ttgtttggta aaaatttcag
    24901 cactgtcttt acgcattttt acaaggcgtt gtaatatttt aatttcatct gcttctgcca
    24961 gttcttcttt tgaaccagaa gccgtttgtg ctaataaaag ttctgattta atagcgcgta
    25021 atgcttctaa agctatggtg tctttagact tcattgcttc tttcatttcg ctcattactt
    25081 ttgctgataa actcatttta tttcagatat tagatttcaa atagtagata ttgagattgc
    25141 aattgatatt tacattgtaa ttctttgcga agttaaaaaa ataaacccga aaatctctag
    25201 gaaactttcg ggttttggta attgtttctt ttttattaat ctacattatc atgaagaaaa
    25261 gaattattag atcgcaactg gctgtcgttg ttgctgtcag ttcctagtga catccttgaa
    25321 gttgaatttg catttgtatt ttcattcaaa tcaattcctg aacgtttgta ggctggttct
    25381 ttttctaatt cttctacttt agaaggattg ttatggaatt tataattgaa ttcttttaac
    25441 tttcttcttc tctcgtctgc tcgtaatttc aaagtttctt caattgtcat ttctactggc
    25501 gaaacatctt caaatgattg accaaaagtt ggtgtttttg tttcttcaat atctttgatt
    25561 ttaatattta aatcttcatc aactggttct tctactttta atattgcagc aactggcttt
    25621 gaattcatta aatcattctc caactccata tattcttcta aagaatattt gacaacacct
    25681 tcttcattta attctgttac agaaacaaat tgtacaggtt cattaactac aatgttttta
    25741 gtttcttcag ataaatcaaa aacaaatcta gtgtcatcaa tttcgttttt tacatcattt
    25801 ggagtttcaa aactgaatgt tatttgttct tcctcttttg aaacattttg agcttcaact
    25861 accttaattt ctttagtttc tggtgtagta attctgaaat cgatatcttt aattggagaa
    25921 acaatttcaa aagtaacatc aagattatta atgaattgcg tcatagcgat taactcatta
    25981 gcatcatcca caacttcaac ctcatttgta taaactgtag tgttttcaac ttcttcttct
    26041 attaattcaa aaacaatttt ttcttctttt tcttcaactt gagagtcaaa ttttgattga
    26101 aaaatatcta ctgatttttg agttaaatct ctaaccaatt tttgctcatc ttctaaagag
    26161 tgaattattt tttttggctc agaatttaca atttcatttt gttgttcaac gtcaaatcca
    26221 gtagctataa tcgttactgc aattgattcg tctagtgatt cgtcttcacc aacacccata
    26281 attatgttag cgttatatcc ggcttcagct tgaatatggt cgttgatttc tccaatctca
    26341 tcaatagtaa tttcatttga accagaaacg ataagcaaca atacgttttt agcacctgta
    26401 attttattgt cattcaataa tggagaatct aatgcagaaa taattgcttc cttcgctctg
    26461 ttttcacccg aagcaataga tgaacccatg atggctgttc cactgtttga aagaactgtt
    26521 tttgcgtctt ttaagtcaat attttgtgta taatgatgcg taatcacttc agcaattcct
    26581 ctagaagcag ttgctaaaac ttcatctgct tttgagaaac ctgccttaaa tccaagattc
    26641 ccataaactt ctcttaattt gttgttgttg ataacaataa gcgaatctac ttgttttctt
    26701 agtctatcaa cacctatcaa agcttgttct gatcgaactt ttccttcaaa ttgaaatgga
    26761 attgtaacaa taccaacggt taaaatatct ctttcttttg ccaattgtgc aattacagga
    26821 gcagcaccag ttccagttcc gccgcccatt cctgcagtaa taaacaccat tttggtgtta
    26881 gtatccaaca ttttttcaat gtcagcaata ctttcaatag ctgattgttg accaacatca
    26941 ggattggcgc ctgcacctaa tccttcggtt aagtttacac ctaactggat tttatttggc
    27001 acagcactat tttgtaatgc ctgagaatca gtattacaca caataaaatc tacacctttt
    27061 attccttgct tgaacatgtg gtttatagcg ttgcttccgc caccgccaac accaattact
    27121 ttgatgacat ttgactggtt tttaggcaga tcaaacgaaa tgtttccaaa ttctgagttg
    27181 cttatcatac tattttggtt tttgttttgt attttgactt aatttttttt ctgtttacac
    27241 aagtttcacg atttgagttt acgcacatct gttaactact aaaactgtta actgagtact
    27301 gaaaaccgac cacaaattac tctgcgttat ctaaaaaatc tttaatcttt tcgacgtatt
    27361 tgtcaaaaaa agatttcttt attttttctc ctgaagtttc ctttggaatt actttttcat
    27421 cttcaaaaac ttcattagga acttcttcca ctttttgttc tggcacagga acatgaactt
    27481 gtcttggagc ttctgtcaga gcatctttca actcaattct gtaggcacta acggtatcat
    27541 ttgcaatgct attcatcact aatcctactg ctgttgcaaa taatggacta gaaatttctt
    27601 gactagagtt gcccgccaaa tgttcgtttg gatagccgat tctggtatcc attcctgtaa
    27661 tgtattctac aatttgtttg atgtgcttca attgagaacc accacccgtt aatacaatac
    27721 cagcaattaa tttttttctt ggatcttcat gtccgtatgc tttgatttcg gtataaactt
    27781 gctcaataat ttcaacaaca cgtgcgtgaa ttatttttga taagtttttt aacgaaattt
    27841 cttttggatc acgacctctc aaacctggaa ttgaaacaat ttcgttgtct ttgttttcac
    27901 ctggccatgc tgagccaaat ttaaccttta atgcttctgc ttgtttttct attatcgaac
    27961 aaccttcttt gatgtcttct gtaatcacat ttcctccaaa aggaattaca gcagtatgac
    28021 gaatgatacc atctttaaaa atagctaaat ctgttgttcc accaccaata tcgattaagg
    28081 caacacctgc ttctttttct tcttgactta ataccgcatc cgcagaagct aatggttcta
    28141 aagttagtcc agacaattca attcctgagc tttgaataca acgaccaaca tttctaatgg
    28201 atgctgcttg cccaactact acatgaaaac ttgcttccaa tcttcctcca tacattccga
    28261 ttggctcttt gatttccgat tgtccgtcaa ttttgaattc ttgtggtaat acatgaataa
    28321 tttcttcgcc tggaagcatt gccaatttgt ttacttggtc tattagcagt tgaatgtctt
    28381 ttccactaat tacttcatca ggattggttc taatcacata atccgtatgt tgaatactgc
    28441 gaatatgttg acccgcaata ccaacgacaa catcattgat tttataacct gaattgtttt
    28501 cagcctctaa aacggcttgt tggatggatt gaatagtttg tgtaatgtta tttaccacgc
    28561 cacgagcaac accaagactt ttggcttttc caatacctaa aatctctagc tttccatact
    28621 catttttctt gcctatcatt gcaacaattt ttgttgttcc aatgtctaat ccaacggcga
    28681 tgttttcttt ttccatacga tacctattta atacaaacca cttgttgtgt gaatttcaga
    28741 tttatcattt tgtatttaat caaagtgcta tctgaaactg ctttttgaaa aaatgcttta
    28801 taattgtcga attttcgttt aatattgatc gttcgaccaa aatctatctt aaaatcgtaa
    28861 tttctattct ctaaaatcaa gctaccatta ggcaaaactt gaacaccaat gatgtttttc
    28921 tgcaaaaact catcttcata aattgtcctc aacacttgag ccaatttttc tttttttaca
    28981 ccagtaatat cccccgaaat gagtggaact cttgctgtat aattgtccga aaacggcatt
    29041 ttatttcctt cataatcaat ataaaaagaa ccattttctg cgcatattct tcccagagga
    29101 gttttttgtt ttaccactgc ttttagaaca ccatctactg ttatataaac atctgctttt
    29161 tgaattaaca attgtttatt gatggaattc tctaatttgt tcaaaactaa agtttctttg
    29221 gtaaaatttt tagcattgtt atttttttct attaacaatt tattaaccgt ttcacttttt
    29281 ataaaaggtg tgttgttgtc tagaattttc acctcaactt tcactatttt tctatgatta
    29341 tttctgtaag atgtgaatga atacaaaaaa atcaccaaaa aaaacatgag tagtaatctt
    29401 atgtttgtcc aacttagaat tttcatgata atgctttttt aatggatgga actaactcac
    29461 caatatcacc agcaccgatg gtaacaatta ctgttgcatc gctttttaaa atggttggaa
    29521 tcaactcgaa ttttgatact aattttttgt tttggttttc aatttttgat aataaccaac
    29581 ttgacgttat gccttcaagc ggtaattctc tagcaggata aatatctagc aacaaaactt
    29641 cgtcaaaacg acttaacttt tctgcaaaat catccgcaaa atctttggtt ctactgaata
    29701 aatgcggttg aaaaacagcc aatacttttt gatttggata caattcacgt actgcttgat
    29761 gaacggcatc aatttctgtt ggatgatgcg cataatcatc aatataaacc agcctttctg
    29821 ttttaatttg atatgaaaac ctgcgttgca cacctttaaa tgaagccaag gctttagcaa
    29881 tgtcgttggt tggggtgcca aaagttttcg ccatcgctaa agccatcaag gcattcatta
    29941 aattgtgatg tccaggcaag ttgaatgcaa tattttcaat agtttctgat ggtgaaatga
    30001 catcaaaaac ataagtacta ttttctattc taatgtttgt tgccgtgaat gttgcttcat
    30061 cattaattgc tactgtcaaa ccttctaaag gcaatccttt tggaacaatt aattttgact
    30121 tatcggaaac cttatccgca aattctcgga aagaagattc aattgatgaa gcatcgccat
    30181 aaatatccaa atgatcggca tccattgaag ttacacaggc aatatttgga tgaagatgta
    30241 aaaaagaacg gtcaaattca tcggcttcca ccaccgaaat agattttcct gagccaatta
    30301 aattggaatt atagttttct acaataccgc caagaaacgc cgtaacatca gcaccacttt
    30361 catataaaat atgcccaaga atacttgagg ttgttgtttt tccgtgtgta cccgcaacag
    30421 cgaaacaaaa agtatccttg gttatcaacc ctaaaacctc tgcacgtttt tttacaataa
    30481 attctcgttc caaaaaataa ttcaactcag aatgtgttat tggcacggct ggcgttaaaa
    30541 tcactaatgt attttcaaca aaataattta caggaattaa actaatattg tcttcaaaat
    30601 gaatattcat accaccagca ataagttcat tggttaatgc tgaaggtgtt ttatcatagc
    30661 cggatacatt tttgccaata ttttggaaat agcgcgccag agcactcatg ccgatgcctc
    30721 caattccaat aaaataaaca ttatgtattt ggtttaaatt cattatttta tcaacttaat
    30781 tatttcttct acaatatctt ttgtagcatt tggttttgct agttctttac aatttttaga
    30841 caaagcattc tgcaattttt catcagaaat taaattttga aaaacttcat caaatttttc
    30901 gtctaattca ctttctttaa tcattaaggc tccatttttt tctacaattg ctatggcgtt
    30961 tttggtttga tggtcttcgg ctacattagg cgatggaata aaaatagtcg gtttaccaac
    31021 caaacagagt tctgaaacag atgatgcgcc agctcttgaa attataaaat ctgctgttgc
    31081 ataaaccaga tccattctat cgataaaatc agttacttgc acattttttt catctgaata
    31141 atgcttgtat tcgtcaaaat aaaactttcc acattgccaa atcacttgaa tgtcttttcc
    31201 aacaatatca aatattttac tttcaattag ctgattgatt cttcttgcac ctaaactacc
    31261 tccaagtacc aaaagtgttt tcttttcaga ttttaattta aaatgttcaa tggcttcatt
    31321 tcgtttatta gaaatatcca ataaatcttg gcgcacagga tttcctgtaa atacaatttt
    31381 atcttttgga aaaaagcgtt ctaaattttc ataggcaaca caaatcgtat tagctttttt
    31441 actcaataat ttatttgtga tacctggata tgaattttgc tcttgcacta ccgttggaat
    31501 gttggcacta tttgccattt gcaataatgg tccagaagca aaaccgcctg ttccaataac
    31561 tacatttggt ttaaattttt taataatgtt tctcgatttc cacaaactgc tcatcaattt
    31621 cataggaaac attgcatttt gaaaggtcaa ttttcgttgt aaaccagcta tccaaagacc
    31681 ttcaatttta taacctgctt gtggcacttt ttgcatctcc attttgtctt tggcacctac
    31741 aaaaagaaat tcagcatcag gaaatctcaa ctttaattca ttggcaatag caatggctgg
    31801 ataaatatgt ccgccagtac cgccaccgct aattatgaat tttagttttt tcatcgttat
    31861 ttatttaaaa ccacatttaa aggattttga ttttcttcta ttgaaaagtc atcctcttct
    31921 ctttcatctt cttcaatttg tttgtcaacc aattttttta acgcagcttc acgcttggca
    31981 ttttcagctt cttcttcggc tatttcttct tcttttttgg ttacatttat tatgattccc
    32041 aatgcaatac aagttatcca aatagaagtt cctcctgaac tgattaatgg caatgtttgt
    32101 cctgttacag gcaataattc taccgcaacg cccatattgg tcattgcttg aaaaattact
    32161 ggaaatccaa gtccaacgac tagtaatttt ccaaaaagag tagtcgattt gtgtgcagcg
    32221 actacaaaac ggaatagcag taaaagatat aatcctaaga caaacaatcc accgaacaat
    32281 ccatattctt ctacaattat ggcataaata aaatcggaag aagattgagg taaaaagttt
    32341 ttctgaacac ttttacctgg tccaagacca taaacgccac ctgaagcaat ggctattttt
    32401 gctttttcaa tttgatagtc atcttcatct ggtttgtctg aagcaaaatt atcaatacga
    32461 cttatccaag tatctacacg gtttggaaat gcatctggaa atgttttggc tactaaaatg
    32521 aaaaaagtta aacccacaat tccagctcca agaataattc caatatattt taacggatat
    32581 ttacctataa aaaccaacat taaaaccatt gaaaaaatca aagctgccgt agataaattt
    32641 gctggtagaa ttaacattaa tgtaacaaat actggcgtcc acaattcaaa aagcgatgct
    32701 ttaaaagtta taggcatttc tctttttttg gataaatatt gcgccacata aatatataaa
    32761 acaatagctg ctaatgttga tgtttgaaac gttattccaa taaaagggat ttgtatccat
    32821 ctactcgcat ttgcaccgtc aatcatggtt ccttttagca aagtgtaccc taataatatc
    32881 caaacaacag gtaaaaatat ccaggaaagt cttttataat aatggtacgg tattttgtga
    32941 acattatata aaattataaa cccaacaaat acttgtgctg catgtttcaa taaataggta
    33001 aatgcatttc ctgaaccatg acgcatataa gccaaattac tacttgcact aaaaacaggc
    33061 ataaatgaaa gcatagccat caaagcaacg aatgcccaga tcactttgtc tccttttaaa
    33121 ctgtttataa agtctttcat aataaacttc tatagattct ttacagctgc tttaaattga
    33181 ttacctctat cttcataatt ttgaaataaa tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33301 nnnnnnnnnn nnatttttaa aactttctcc aaacgatgct caacaccttg aaaatttgat
    33361 aaactttcgc gaatggtttc ttttctgatt ttcatcagtt gtgctactga tgtagctgcc
    33421 atagcgtttt tcaaattgtg atttccttct aaagaaatat ctacaatttt catttccaat
    33481 tcttcttcgt tgataagaat gttcatagtg tcttgtttta taaatgctcc aaattcaaat
    33541 tgttttgaca gtgaaaaagg aactagtttt gcttttgttt tgttttgttt taaccattca
    33601 gaaattgctt catcatctgc gtcgaagatt aaaaaatcat cttcggtctg gttcattgta
    33661 attctaaatt ttgacgcgat ataattttcg tacttataat cgtatcggtc taaatggtct
    33721 ggactaatgt ttgtaattat ggctatttct ggcttatagt caataattcc atccaactga
    33781 aaactgctta attctaatac atagcaatcg aaattttctt cggctacttg ccaagcaaaa
    33841 ctttttccaa cattacctgc taaaccaaca ttcaatccac ccgattttaa caaatgataa
    33901 gttaacattg ttgtggttgt tttaccatta cttcctgtaa ttccaatagt tttagccgaa
    33961 gtatattttg ctgcaaactc aatttctgaa ataactggta ttcctttttc caaaagtttc
    34021 tttacgattg gtgatttctc tggaattcca ggacttttca ttactacagt tgcattcaga
    34081 attaaatctt cagtatggtt ttcatcttcc catttaattt catactgatt aagaacgtct
    34141 ttatagtttt cttttatttt tccgaagtca gaaacaaaaa cttcgtatcc ttttttcttt
    34201 cccaaaatgg ctgttcctac tccactttct ccgccgccaa gtattaccaa tcgttgcatt
    34261 ttatctcaat ttcaaggtta caatagaaac gatggcaagc attatggcaa caatccaaaa
    34321 acgggttacg attttacttt cgtgatatcc tttcttttga taatgatggt gtaatggtga
    34381 catcaagaaa attcttctac cttcaccaaa acgtttttta gtgtatttga aatagctaac
    34441 ttgcatgata acagagagat tttcagcaaa aaagatggca cacaaaatag gtagaagcaa
    34501 ctctttacga accgaaattg ccaaaacagc tataattccg ccaatagtta aacttcctgt
    34561 gtcgcccata aatactgatg ctggatagga attgtaccaa agaaatccga ttagagcccc
    34621 aacaaaagct gaaataaaca cagtcatttc tcctgaattt ggaatgtaca ttatgttgag
    34681 ataattggat aaaatgatat tacctgatac gaacgtaaaa attccgagtg cgagcaccga
    34741 aatagccgat gttcctgctg ccaatccatc gattccatct gttagattag ctccgtttga
    34801 aactgccgta atgataaaaa taacaattgg aatgaatacc aaccaagccc aattttgata
    34861 ctcatccgta aaaggtttta aaatttgagc ataatccaat tcgttgtatt ttgtaaaagg
    34921 aattgtagtt gttgttgatt ttatttcact tggcaattca ttgatgactt tttcagaact
    34981 attaaaagga acagcattat tagtatgttc tcttaccgta actgcaggat gaaaatacaa
    35041 aaccgaccca acaattaatc ccaatccaac ttgaccgata actttgaaaa ttccttttaa
    35101 cccttgttta tcttttttga aaatcttgat ataatcgtca ataaaaccga tagttcccat
    35161 ccaaagagtg gttacaatca ataataaaac gtaaatatta tcaagttttg taaatagcaa
    35221 aacaggaatt aaggtagcaa caatgatgat taatccaccc attgttggcg ttccagcttt
    35281 ttgagtttga ccttcaagac caagttctct aaccgtttca cctacttgct gatttctcaa
    35341 aaatttaatt attttttttc cgtagattgt tgacaacatc aacgatagga ttaatgctaa
    35401 tgcagaccga aacgtaatgt attggaaaac acctgctcca ggaatatcca ttgttttatc
    35461 caagtattca aataaatagt ataacatata tttatattaa attccaattt ttaaaattcc
    35521 aaattccaaa gttttactga aaatattggg atttgggatt tttcttttgg tgttttttat
    35581 tttttcattt gttctaaaaa ttcttttact attttcatat catcaaaatc gtggcgaaca
    35641 cctttgatct cttgataggt ttcatgacct tttcctgcaa ttaaaataat atctttgaaa
    35701 ctagccaatt gacaagcggt tttgatggct tgttttctat caacaattga aagtgttttt
    35761 ctttgatttt gaacctctac acctttttcc atatcggcaa taatatcaat aggatcttcg
    35821 cttcttgggt tatctgatgt tagaattact ttgttactca ttgatgaagc aatatgcgcc
    35881 ataattggtc tttttgcttt atctctgtcg ccaccacaac caataaccgt aattaaatgt
    35941 tcgttattcg ttctgatatc attaatggtt ttcaacacat tttctaaagc atcaggcgta
    36001 tgagcataat caacaattgc agtgatttgt tcttcagaaa ctataaactg aaatcttcct
    36061 gaaacacttt ctaactctga aagtaatcgt aaaacttcta acttatctaa accaagttct
    36121 accgcagttc cataaattgc caataaatta taggcattaa acgttccgat taatttcacc
    36181 caaacttcac tttcattaat tttcaacagc aaacccgaaa gttggttttc aagaatttga
    36241 gctttataat ctgcataact ttttaaggca tacgtcaatt ttcttgcggc tgtattttgg
    36301 aacattatgg ctccattttt atcatcaaca tttgtcaatg caaaagccgt ttttggtaaa
    36361 tgatcaaaaa atgatttttt cacatcgcga tattcggcaa atgttggatg ataatctaaa
    36421 tgatcatgag acaaattggt aaaaactcct gctgcaaaat gtaatccttc agttcttttt
    36481 tgatgaatac catgcgaact aacttccata aaacaatagg caacaccaac ggtattcatt
    36541 tctgccaaat aatgattaat agtcaacgaa tctggtgttg tatgtgtagc tttatactcg
    36601 atttcatcta tcattatttt tacggtagat agcaatccta ctttgtaacc agctttttta
    36661 aacaattggt ataaaagcga cgcaatagta gtttttccat tagtaccagt aataccgatt
    36721 aatttcaaat tgacggaagg gtttccataa taattggatg ccataaatgc caaagctgag
    36781 ttggtatctt ttacttctat ataagtaact cccgaaacaa taatttctgg caaagtgtcg
    36841 caaactatgg cgactgcacc aagatttata gccttttcaa taaagtcgtg tccattagaa
    36901 actgttcctc gaatagcaac aaaaacatcg ttttcttgca cttttcgaga gtcgaattca
    36961 attttctcaa tagccatttc tgttgaccca tgaaccgatt cgattgctac tttatacaat
    37021 atgtctttta aaataatcac gacaattcga gtgttatggt ttttatattt tcaagcaatt
    37081 ctcctggcaa aatggattgt tttttaactt ttccaatacc tattactttt acttttattt
    37141 ttaagttttc aagaaatgct actgcatcca ttcctgccat tccttttaag ttgggtaatg
    37201 tttttttatc ggcttgtatt ttatcattat attcttgata ggcaacttcc actttttgga
    37261 ttttagtttc taggttttcg atttctttta tggatggcga atcggtaaaa atcttttgtg
    37321 ctattcgctt aaaaactggg ccagcaacgt cagctccata ataattgttt ttagaggtgt
    37381 ttggcttgtg caccactaca atgcaagaat atttgggatt ttcaactgga aaaaaaccga
    37441 cgaaagatga aatataatat ttgtcagctc caccattcga tccataatta gctgcagctg
    37501 tacctgtttt tcctgccatt gaaaaatctt tagaatacaa ttttgaagca gttccgcgtt
    37561 taaccacatt tcctaaaact gctttgactt ttttgagcgt ttcgtccgag caaatttttg
    37621 gattgataat ttctgtattg tattttttaa tgactttatt ccattcttta atttctgaaa
    37681 caaattttgg tttaatcatt acaccattgt tcgctacagc attgtatagt gccaatgttt
    37741 gcaaaggtgt aaccgaaact ccataaccgt atgccatcca aggcaaagaa agcccactcc
    37801 actttttatc acttggttgc ggaatataag gaacaccttc accttcaaaa ggtaacccga
    37861 taggtttatt caaacccaat ctatcaattc tatctacaaa ttcttttggg tcatctttat
    37921 aattttcata aaccgcttgc actaataccg tgtttgaaga cacttcaaaa ccacgtgcta
    37981 gtgaaatttt accataaccg ccatcgtgag aatctcgaac ttttcttcca taataagtta
    38041 caattccacc attggtatca taaactttac tggtgtcgat tttattatca tcaagcaagg
    38101 ctattaaatc tactaattta aaagttgagc ctggttcatg tgattcggct accgcataat
    38161 ttacggtttc ataataggaa ccatcattag ctcttcctaa attagaaatt gcttttacat
    38221 ctccagattt agtttccatc acaactacac atccatgatc agcttcatat agttctaatt
    38281 gctttaacaa ggcatgatgt gcaatgtctt gaatgtaaac atcgatagtt gaaatcacat
    38341 cataaccatc ttgaggatca acttcattta tatctcgaat aggtttccac tgtcctttag
    38401 caattttttg ttttactatt ttaccatctt ttccgttcaa atagtttcta aacgaccatt
    38461 ctattccttt tccatctgga tgtccagccg aagtaactct ttcataacca atagttcttt
    38521 ctgctatttt acctattgga tgctcacgaa ccgttttttg ttcggtaatc attccacctt
    38581 tataagttcc taaattaaaa agtggaaaac gtttcattct taaatattct gtgtaactca
    38641 agcttcgtgc taagagataa taacgatttt tatcggcttt tgcttttctt aattcagaat
    38701 gatagtaagc acttggtttt cccaacatta atgacaatga atcagataga gcttttacat
    38761 ttgcattaaa gtcttcatct tttggagcaa cggcatcaaa ccgaattgta taattgggaa
    38821 ttgaagtagc taacaaacta ccatccgcag agtatatatt tcctttattg gctggaatta
    38881 taaaattctt aaccgttctt tctttggcta attttcgata aaaatcacct tgaacccatt
    38941 gtatttcagt tagcttaaag gttattgcac atgcaaccaa aaagaagcta actgctacta
    39001 ggtatattcg atatgatatt tgcttatcct ctattgccat atctttttaa agaaactttt
    39061 ttcttctatt tttttaactt ttattttttt tggtggtacc gacgatggaa aaatgtttcg
    39121 ttcttccatt ttttgagaaa tagtcgattc catttttaat ttcattaatt ctgagcgtct
    39181 atcaacaaat tctgaacgca attcttttac ttcatttgtc aaggctgtta ttctgaatac
    39241 tttttggtca aatcgatggt tattggcaat cataaaaatg gctaataaga ttaggaaaac
    39301 gataaaacgc caattttttg ttgcgtcttc gttgactaat attcttgctt ttaatatacc
    39361 ataaactcct tttttcatca acctttattt tttttcagct attctcaact tggcacttct
    39421 tgctctatta tttattttaa tttcagcttc tgaaggaata atcatttttc caatcaactt
    39481 aaaaggaaca gaaaagtttc caaaaaaatc tcgttcaggt tctccttcaa atagtccgtt
    39541 tttcatgaaa cgttttacca atctatcctc taaagaatga tacgaaatca cacttaatct
    39601 tccttctggt tttaatattt caagcgattg ctctaaaaat tctttcaaaa cttccatttc
    39661 ttgatttact tcaatgcgaa tggcttgata gatttgagcc aaaattttat ttttaaattg
    39721 ctctggcaaa tatttattca agacctgttt tagttcttct gttgtttgga taggcttact
    39781 ttctctagct tcaagaattg tttttgccaa agctggagca atttttaatt cgccataatc
    39841 caaaaaaact cttcttaagt tggcttcatc ataatgatta accactttaa aagcattcaa
    39901 atcatttttt ttgctcattc tcatatccaa atcggcttca aaacgtgttg aaaaacctct
    39961 ttctggaaca tcaaattgat gcgaagaaac acccaaatca gcaagaattc catccacctt
    40021 ttttactcca taaaacctaa taaaccgttt gatgtatcta aaattctcag gaatcaaaac
    40081 aaatctttca tcatcaaaca cattcgcctg cgcgtcttca tcttggtcaa atccaaataa
    40141 cttccctttt gggccaagtc gttttaaaat ttcacgagaa tgtccgccgc cgccaaatgt
    40201 tacatcaaca taaacaccat caggattgac attcaagcca tcaactgttt cttgcaacaa
    40261 aacaggatta tgatattcca ttgtcgtcat ttaaatttcc cattacatct tctgccaaat
    40321 ctgcaaagtc aacatcatca ccagaaattg atttttcata caactctttg tcccaaatct
    40381 ctacaatatt caccgccgaa gaaaggacaa tgtctttgga aatagttgaa aatacaatca
    40441 aatctttcgg aatcaataat cttcctaatt catcaacctc aaccatttta actcctgctg
    40501 tgaaacgtct gataaaatca ttattctttt tcacaaaacg atttagctta ttaattttaa
    40561 gcatcatcaa agtccattct gccattggat acaactctaa acacggttga aacaccgaac
    40621 gtttcaagac aaaaccatct tttaacgaag gagccaattg cttttttaat gccgcaggaa
    40681 tcatcaatct tcctttagcg tcaagtttgc attcgtatgt tccaattata gaatccactt
    40741 taatttgttt gattaagagc aaaaatataa aaatatttac cacaatttac cacaaaatac
    40801 cactttgtta ataagtttta ccacttcacc aaaaaaattt agtttaaata ctgttacgta
    40861 aatttgattt tttttaagga ttttttaaga atctcagtaa cagcaaccct ttgaattaat
    40921 atgcatactt tttataatca caaaaacata tctacaaacc attaaagaca cattctcaaa
    40981 aaatcaacta tttcttaata ttgttgataa ttaaagattt aaaaaaagta aaatataaaa
    41041 cgacctagtc aagaaaaaga aatttgaaat actcccaatt tagttagtat ttaattcaga
    41101 aaattatata tttgtcttat tcggaaatta gcgattttta atggaaaaaa tatacaagaa
    41161 agaaggcaga tattcatatt atgaaactgg cgaaggtaca ccaatcgtag ttttgcatgg
    41221 attaatgggc ggattaagta atttcggaga agttgccgat tatttttcta ataatggcta
    41281 taaagttgtg attcccgaat taccgcttta tactcaaaac atattaaaaa cgaatgtgaa
    41341 agcatttgca aaatatgtta aagatttcat cacttttaaa ggttttgata gagttatttt
    41401 attgggaaat tcacttggtg gacacattgc tctatatcac tcaaaaatgt atccagaaaa
    41461 agtagctgga attgttatta ctggaagttc aggtctttat gaaagcgcca tgggcgatag
    41521 ttatccaaaa cgtggtgatt atgaatacat aaagaacaaa acagaagttg ttttttatga
    41581 tccaaaaatc gctaccaaag aaattgttga tgaagttttt gccattgtaa atgaccgaat
    41641 aaaagtaatc aaaaccttaa caatagccaa aagtgctatt cgtcataata tggcaaaaga
    41701 tttaccaaag atgcacatga aaacttgtat catttgggga aaaaacgaca aagttacacc
    41761 gccagaagtg gctgaggaat ttcataaatt aatgccaaat tcttctttat attggattga
    41821 caaatgtggt catgctgcca tgatggaaca tcctcaagaa tttaatagta ttttgcacca
    41881 atggttaaaa gaagtacatt tgtaacatca taaagttcct gttttggaac tttttttatt
    41941 tttaatatca tggaaataca taccgccgaa tttataatta gcaattcaga agtaagcaag
    42001 tgtcccaaag aacctttgcc agaatatgcg tttattggtc gatctaatgt tgggaaatct
    42061 tcactaatca acatgttgac caatcataaa aacctggcaa aaacctctgg aaagccaggt
    42121 aaaacgcaac taattaatca ctttaaaatc aatcaaaatt ggtttttggt tgatttacca
    42181 ggatatggat atgctagagt ttctaaaaaa acaaaagaag tttttcagaa attcataacc
    42241 gactattttg aaaaaagaga acaattagtt tgtggttttg tattgattga cattcgatta
    42301 gaagcacaaa aaattgattt agaattcatt acttatctag gtgaaattca agttccattt
    42361 tgcatcattt ttacaaaagc agataaaata agtaaaacaa aaatagattc gcatattgca
    42421 gcctatcgaa aagcattatt ggctaataac tgggaagaaa tgccgcaaca ttttgtaact
    42481 tcttctaccg aaggtactgg aagaaaagaa ttattagaat ttattgactc tgtaaatcaa
    42541 gagatgttta aggataacgg attttaaaaa aagcggtcaa atgacctctt tttatttagt
    42601 taactccatt ttttcggcaa aatattgaca aaaatctttc atagtttctg ccattttttc
    42661 atcagcagtt gcgcgttgaa aagtatctgc catagctact aaagtttgat gaaaaaactg
    42721 tttcatttca tcaacaggca tttctttcgt ccacagatca attcgcaagg tttctttggc
    42781 ctttgcgtcc caaatagaaa gcatcatggc tttagcttct tccaactcaa ctccaccatc
    42841 ttgagccgtc caagttaatt tttctggaac tctattttcg tctaattcta ctaaaaattt
    42901 tatttctgaa gtcatttttt aaggttgtag ggtttaaggg tttaaaatat caaaaaatca
    42961 tgttaacaat gaaatttcat aaaaaaaagg tcaacctaat ttaaggattg gcaggttact
    43021 gcttactgaa cactttattc ctttttcggt ttatacttag agcggtcaaa aatgtcttta
    43081 tattccattt ttaacaactc ttgaacagtt actttttcgt tatttttcat aaatgaacga
    43141 acaatttgcc atccaatcca ttgtccgact ctacctggag attcgttgtc aatttctaaa
    43201 taaaatttag aaaatggtgc taaatttata aagcggtttg gtagttttgg atcggagtta
    43261 tacagcaaac tctcatcaat aaaaaaacgc cacatataac tttcgttttc ttgacaccaa
    43321 gcaatttgtt ctggcaaata tccaattttt tcagcgtcgg tataatttgg taaaagtagg
    43381 tcttttaaga acaactcttt accataatat atcatttctg ctaaaagcga ttgttctttg
    43441 gatggcggat ttgttcgcat tgaaaaactg gaaactatat cgggcatcat ttgtctttct
    43501 tcaaaatttt gtttgatata ttcgggaaat tcataaaatt tatgttcttt tcctaaatac
    43561 aattctaaag caataattaa tttatcattg gcataaattg ccttattgtt ataatccatt
    43621 tctccaataa ctgtataaac tgttggcgta ataatggttg ggaaataata tttcaaatgt
    43681 ttgaataaat cttcaatttc ggtagtttgt tttccaaaat ttgagtattt tttttcaact
    43741 tcggtatata attcgcgcca aagtggattt tgcatcttct ctaaccaaac gttgtcgtct
    43801 tcattttctg gaaagaaaaa agggtattca gctttcaaat tttggagttc cgaaggtttg
    43861 caatcaaaaa aatctttttc aaaacgaact actttaattt ggactggaat ttcctcaact
    43921 gctttttcta cttttgattt cttatcgcaa gaaataaaaa ataaaaacaa aattggaatg
    43981 ctataaaaat actttttcat tcgtctaaat ttaaatcaaa tcctaaataa ttactatttt
    44041 tgatttttta actttgcaaa tatacatttc cgtttcttaa aacaatccta acaaatgact
    44101 aaaaaaagtt caattaatgt tgaaaaagtt aaccaacaca ttgttaactg gctgaaagaa
    44161 tatgcaaaaa aagcaaaagt aaatggtttt gtagttggaa tttctggagg aatagattct
    44221 gctgtaactt caacattgtg tgcgcaaact ggttttaaaa cactttgtgt agaaatgcca
    44281 attcatcaag acttaaaaca agtcaataga gggaaagaac atattgaaca attgagaaac
    44341 agatttccta atgtttctca tgctattaca gatttgacac aagtatttga aatttttaaa
    44401 agtgaagttc caaaaagtga tgatgcaaca aaatacaatt tgtcattagc caatactaga
    44461 gctagattgc gaatgacaac tttatattat catgctggaa taaatggatt tctagttgct
    44521 ggtaccggaa ataaagttga agattttgga gtaggatttt atactaaata tggtgatggt
    44581 ggtgttgacc taagtccaat agccgatttg atgaaaagtg atgtttttgc attagcggaa
    44641 tatcttaaag tacctcattc aattcaaact gctgctccaa cggatggttt gtttggcgat
    44701 tcaagaacgg atgaagagca actcggagca agttatgacg aacttgaatg ggcaatggtt
    44761 gaagttgaaa aaaacaaaac tattgaagat ttttttggcc gtgaaaaaga agttttttct
    44821 atctacaaac gcctaaattc agccaacaaa cataaaatgc aacccattcc tatatgtatt
    44881 ttgccaaaaa aacataataa aaatgacgat taatagtatt atatttattt ttttattatt
    44941 tttactctaa atttctatta atctttatca aaaaccacaa ttatgataaa tgtatgtatt
    45001 gctgataact ttccagttgt acactttggt atcaaagctt atttcaaaga ccacaatgaa
    45061 ataaacattg catccaatgt aggaaatttt tttatgattc cagatgcatt aagaaacaaa
    45121 gaaattgacg tcttaatctt ggatttagaa cttgacggat taaatagtat ttatgaagta
    45181 aagtcaatca ttaaaaattt tccaaaaact aaagttatta ttttcagtag tttgtctgaa
    45241 cacatttatg caccaaatgc tattaaagca ggttgtgccg gttatgttca taagacatca
    45301 aaacttgaga atttagggat tacaattgtt aaagttaaca atggtcagat tatcctaaat
    45361 gatgacatca agaaaaagct agcattaatt gctaaacaaa acaaaacgga acgtttgtac
    45421 cgtaaacttt ctaaccgaga aatcgaagta cttcgttttc ttagtgacgg aaaaaagaac
    45481 aacgaaattg ctaagatttt gaatcttaat gaaaaaacga ttagtactta taagctccgt
    45541 cttttgacaa agctgaatgt aacgaacttg gttgaccttg ttaataaagc aaaaacttta
    45601 gaaatcgttt aaaaatttaa aaatcccgag aaatcgggat tttttttaat aatatgtaga
    45661 tcgaattctt cctattttat tggtcaacat cactttcaat ttattataat cattaatatt
    45721 tgcctttaat tcttcaacat ctatttcttc atttgttgaa aaatcattca tcaaatctgc
    45781 tattacttta tttattaaat attctctaaa cgatattatc gtttctgaaa catattgaga
    45841 tatcgattgt tctttttgtt ttacataaat gtgtttggtt tcccagttat gtagagattc
    45901 tttttcttca ttcatcaaaa tatctgtaac aatttgagat tgttcttcag ataattgttg
    45961 taaaaattgt tcttgactaa aactttcttc ttgattgtat ttttcaataa gagtactata
    46021 tatatcctta aaaattggat tagccaattc aatttcatct tcttgcaaac tcaagtaaat
    46081 cctttgaaaa actttataat tatttttctc aggaatttca atagtttcgc ctgtatcatt
    46141 tgtcttcaaa ataacatctt caaattcggc atctactgtt ccgtaaatca ataatatttc
    46201 tatcaattta cgttctaatt cataaataac atctacttta tgagcaacaa cttgcgtgtc
    46261 attctttaca acttcaaacg ctttttgctc agtgttctca gtgttttttt gtttttttcc
    46321 agcttctaaa atatctttct taacaatttg cgccaaagta ttcaacaaaa cttgctcgga
    46381 aatatccata attcgtgaac attcctggat atagatttct cttttaattc tatctggaat
    46441 tttggaaata ctaacaacca tatcgcgaat taaatcggct tttttaattg gatcgttctt
    46501 ggcttcatcc atcaaaatgg aagctttgaa ttggataaaa tctttggcat tgttttccaa
    46561 ataagccact aaatcgtcat aggaattttt tcgagcaaaa ctatccggat catcaccttc
    46621 tggaaatgtg caaactttga cgttcattcc ttcttcaaga atcaaatcaa ttccacgaat
    46681 ggaagcgcgc aatccagcgg catcaccatc aaaaagtact gtgatgtttt tggtcaaccg
    46741 attgatcaat cgaatttggt caggtgttag tgctgttccc gatgaagcca caacattttc
    46801 aattccggct tgatgcattt gaataacatc ggtataacct tcgactaaat agcaattatt
    46861 ctgcttggca atagcttgtt tggcatgaaa aattccatac aacactttgc ttttatggta
    46921 aatatcgctt tcgggtgaat taaggtattt tgccgctttt ttatcattgg ttaaaattct
    46981 tccaccaaag cctaaaactc ttcctgacat cgactgaatt ggaaacatca cacgaccttt
    47041 gaaacggtca aattgtttgt cttcttttac gatagtcaga ccaactttat ccaaataatc
    47101 caactgatac ccttttgaca atgcttcttt tgtaaacgca tcccaagttt ctggcgaata
    47161 tcctaatcca aactttgcaa tcgtatccgt tgtaaagcct cgttctttaa aataagaaag
    47221 tccgatggct ttaccttctt ccgaattcag catggtttct tgaaaatagg ttttggcaaa
    47281 ttcagacacc aaatacatac tttctttttc gctggcaatg gctttatctt catttgaaac
    47341 ctcggtttct tcaatttcta tattgtattt ttttgccaaa aaacgaatgg cttctggata
    47401 ggtgaaatgt tcgtgttcca ttaagaaagt aactgcgctt ccaccttttc cggtactaaa
    47461 atctttccaa atttgtttta caggcgaaac cataaacgaa ggtgttcgtt cttctgaaaa
    47521 tggacttaat cctttaaagt tacttccagc acgctttagc tgaacaaagt cgcctatgac
    47581 ttcctctact ctagcggttt caaatacttt atctatggtt tctctggtaa tcaatttttt
    47641 ttataaaaag tttagtgttc aaagttacat gataaaataa aaaaaaccta caagcttttg
    47701 aaaacttgca ggtttcgaat atatagctaa ccctaatcaa acatttatta attgaaatcg
    47761 aaacgaacac ctaaagaaac attgttttgt ttcacggcat tgtctttaaa tagcggattc
    47821 aaatcatact ttacatacaa actagtagct ctataaccta tatatgcact caatccatat
    47881 ataaaattac tagaattaaa atcgcctttc gttttctggg ttacttccat atcgttacta
    47941 ttatattcta taatttgttt cgatttaact ctaacacctg catagccacc aagccccatc
    48001 ctaaatgatt gatgtgtttt aaaataggtt ttgtcttcct taattgtttt gcctgaaaaa
    48061 tcaaattcta aatgcaaagg caacactaaa tatacatttc taaaacgtga ttctttcaaa
    48121 tttaacccac tgtcttccaa attggtttga tttccatttt ttacaaacat tctgttttca
    48181 gttggtctca agttgttgta catcactgac attccatatt ttaaatgtaa taaattgtta
    48241 ttttttgcaa ttcttgtgtt atacgttaaa ccccattcgt aaaagtgtga accccaaaat
    48301 ctaaaatctg aattttcaat attaccttca gtaaccacat tatttaatcc agcagcaaaa
    48361 acaaattgtg atgtggttcg tctttcggaa taatcttttt tctttttaaa gctaactgta
    48421 tctttttcaa attttaaaac ataacgattg ttaatcctaa ctgttgtaga atcatttgat
    48481 ggtttggaca ctatttttcc attaaccttt ttttgcacca attcttttaa ctcttcatga
    48541 gtaacagcca ctttattttc tataattatt gcacgtgctt cggctaattt tttcttttta
    48601 tcgtcggctt cagctttagt tattgttccg ttttcaaatt gtttgtttac ttcttcaatc
    48661 tcaattttta gtgcttcttt ttcgtcttta gttatttttt caattttttc ggcaatttgt
    48721 cttgctttag attcaaaagt ttcttgtgcg gtaatttttc ctgctaaaag gcatgccgct
    48781 actgctaagt aaatggtaaa atttctcatg attgtttgtt ttaaaatgat taatgattgt
    48841 tattcttgat ttctattgct taatgcaact tttacttctt tataattttt actaatactg
    48901 ttaatgactt tttctctaaa agtttgttct acttcgccgt cagcatgtga taaaagcgta
    48961 ttagcatcaa cttttatttt gtctaatttt gatttagctt gaaccttact agaattttcg
    49021 gccgaagcaa gcaacgcatc tacatctaca taagttgatt tcttttgaac taattctttt
    49081 cttggttgaa ctactattgg ttcagtagaa ttaacttttg gaatgtcttt tactgctgca
    49141 acatcgccgg ctactaaata ttctatttct ttgtcacgat tgatgattga attttgattg
    49201 cttttttgat tgatgattga aacaccttga ttgctgattg ctgatttttg atttttgatt
    49261 tttgatggat tattagttgt tgctacttga ttttcattag ttattggtag ttcgtcattt
    49321 attttaggtt cttgattttt tggcgtaact actatttcat cattaggaat tgaaatgctt
    49381 ttttcttgat tgaatataaa caaaccaact gttagcaata ccaaaacgct tgcagcaacg
    49441 ccaataaacc aaaacggaaa acgctttgtt tttttttctt cggcaacagt aagcattgcg
    49501 tccaatctat cccaagcttg tgtagaaggt tgaatttctc ttgcattcaa cttttcttta
    49561 atttgatttt ccatattatt cggttccatt gtaattcctc tttaatattg tttttctagt
    49621 ctcattgaat tcgcaaacaa ataatctata ttattgatta tttttcttga tgctcatttc
    49681 gtaattttta agttttgtca cattttgttg aagaatcctt cttgcgtgtg ataattgtga
    49741 ttttgatgtt ccttcgttaa ttcctaacat tgctgcaatt tcatgatgtt tataaccttc
    49801 aatgcaatac aagttaaaaa ccattttata tccatcgggt aaactatcta tcaaaaactg
    49861 aatgtcttct attgataatt ggctttctat attgttgacc ttttcttctg tgaaattttc
    49921 atcttctaag aactttactt ttttttgaac tcttataaat gaaatacatt cgttaatcat
    49981 aattcgtcta atccaacctt caaaacttcc ttcgtgtttg aaatttttca gattcgtgaa
    50041 aactttcata aaagccgtaa tcattatatc ttctgcttcg tgaatatctt ttatatattg
    50101 gcgacaaatg ctaagcattt ttggagaaaa tttagaataa atcttttgtt gcgcatgacg
    50161 attattttcg actgctagtt cgattaattc tttttcttct tgatgtaaat taattacttt
    50221 catttagatg gttgagtttc tatctgactt ctattaacat agacgaagat aggttcaaaa
    50281 tagttgcatg gtaatttgaa aattattgaa tttgaaaatt tgaaaatgcg taaaaatcac
    50341 attaacaatg ctttattgaa aaaattattt tgaaaaaatt atttttttct ttcaataacg
    50401 taattaacca ttaactctaa acttgattta tactctgaat tggggaaatt ttgaagtaat
    50461 aaaagtgctt cttgttgaaa ttcgatcatt ttttgttcgg caaaagctag tccgtttttg
    50521 tctttaacaa attggatgac ttctttgacg cgttttttgt ctttgttgtg gtttttaatg
    50581 gaattaatgc accaactttt ttcttttgaa gtacaattat ttaaagcaaa aatcaacggt
    50641 aaagtcattt tttgttcttt gatgtcaatt cctgttggtt ttccaatggc atcatcggta
    50701 taatcaaaca aatcatcttt gatttgaaat gccattccaa tcagttcacc gaactttcgc
    50761 attttttcaa ccaaaacttc gtcttcggaa accgattttg cacccaatgc acaacaagcg
    50821 gcaattagag ttgctgtttt ttgacgaatg atttcataat aaacgtcttc ggtaatatca
    50881 agtcggcgtg ctttttcgat ttgcaataat tcgccttcac tcatttcacg aactgctacc
    50941 gaaattatac gaagcaaatc aaaatcgcca ttatcaatag aaagcaataa ccctttagac
    51001 aataaataat ccccaactaa aacggcaatt ttatttttcc aaagtgcgtt aatagaaaaa
    51061 aaaccacgtc ttctgttgct gtcatcaact acgtcatcgt gtactaaagt tgctgtgtga
    51121 atcaattcaa ttacacttgc gcctcgatag gttctttcgt ttacttttcc tccagaaacc
    51181 attttggcaa ccaaaaacac aaacattggt cgcatttgtt ttccttttcg atttacaata
    51241 taataggtaa ttctgttgag caacgccact ttagaagtca ttgattcgta gaactttttt
    51301 tcaaaaagtt ccatttcttg ctgaatgggt tgttttattt gtgaagttat attcatctgg
    51361 gtacatccaa tttacattga atgttcaaat atacaataaa gcgtgtgttt gtgaaatttt
    51421 ttaagtataa gactaaaaac aaaaaacctt caatttcttg aaggttttag agcggaagac
    51481 gaggctcgaa ctcgcgacaa ccagcttgga aggctggagc tctaccaact gagctacttc
    51541 cgc
//
LOCUS       DAOOBH010000006        49095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_887_length_49095_cov_7.378691, whole genome shotgun sequence.
ACCESSION   DAOOBH010000006 DAOOBH010000000
VERSION     DAOOBH010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 49095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49095
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_887_length_49095_cov_7.378691"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PK218_01585"
     CDS             complement(<1..422)
                     /locus_tag="PK218_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPW97233.1"
                     /translation="MIDKIKEHIEEAKAFNEKNKEALEQFRIKYLGSKGLLKELFTEF
                     KNIPNDQKKDFGQVINTLKAVAEEKVKTIQEELESKEEVKGFYGDLSRPGEPLTIGSR
                     HPISLVKNQIIDIFSTIGFNVSEGPEIEDDWHNFTALN"
     gene            complement(531..887)
                     /locus_tag="PK218_01590"
     CDS             complement(531..887)
                     /locus_tag="PK218_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97234.1"
                     /translation="MSRDIELKNRWDKVVEILSNQFADGDLLDLDAIIYLIGVQELGQ
                     FKRVFAKDEKVNLMHIGICRLLEPYGFYEFNFTDKEGWPHYKIKEELPPLKAGEQSIL
                     MKEAIVNYFIEKEVIE"
     gene            1001..1504
                     /locus_tag="PK218_01595"
     CDS             1001..1504
                     /locus_tag="PK218_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPW97235.1"
                     /translation="MSYIDIILGGLLIFGLIRGIKNGLFVELASLLSFFVGIYLAVKF
                     SYLVGGFIGDSKSAKVFAFIITLIAVVVGIHLLAKLFSKIANFVFLGWLNMLGGAVFA
                     VLKTAILLGIVLSLFQKVNIDNAIISKETQESSIFFNPVMKTSEVLLPVLTNWFNDLK
                     ENVKEEN"
     gene            complement(1501..2247)
                     /locus_tag="PK218_01600"
     CDS             complement(1501..2247)
                     /locus_tag="PK218_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW97236.1"
                     /translation="MKNILYILLIFAQTLWAQSDFETGNALYAKEKYTEATQSYENII
                     NSGKQSAEVYFNLGNCYYKLHQVAPAIYNYEKALLLNPNDTEIKTNLEFARKMTIDDI
                     KVIPKVGFHKIIADFTSTYHYDTWAWIAVGLSFLFFVFFLGYYFSGTTVKKRLFFSGM
                     FLVLLAISLSVSSGIYERDRMLNEKPAIVFAETTSLKSEPKISATDAVLLHEGTKVFI
                     MESIANWKKVQLTDETTGWIESEAIKEVKN"
     gene            complement(2249..4015)
                     /locus_tag="PK218_01605"
     CDS             complement(2249..4015)
                     /locus_tag="PK218_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPW97237.1"
                     /translation="MKRILFLTVILISNLLCAQVEFEAKVSKNSLGLNEKLRVDYTMN
                     ADGDNFVPPNFEACGFKVVGGPSQSISQSWINGKSSFNKSYIYILMPLQKGTLTLKQA
                     SIEIGGQTYKTTPVKINVTNAVDLPRNPDEAPAISADDNLYLVADISNSNPYVNQPIT
                     VVYKLYFSYNIGISNWRELSKPKYNDFWSQNIDIKQLAAEEGMFKGERYRYVVLRKTV
                     LYPQKSGKLEIEPLSLDIDCQVPTNRRNFWGQPLMTEDSKRVSAGSKVITVKSLPNEG
                     KPLDFGDAVGKFDFKVTPSKTTLKSGESLDLTVKVVGTGNLKLFSLPKPVVPSSIEMY
                     DPEHNENVSTPLSGMTGSISDKYTLIPQFKGNYKIKPLTFSYFDLDTKKYKTIESPEI
                     VINVLDGPNDAIAASNSPKTSEKNTIEKTKSFAYIKQKTTLSPMEEDDFLGSGLFYSL
                     VFLPFLAIPLVVLVRKKKEAIDNDVVGNKIKKSSALAKKYLSEAKKQLNNKEPFYVAL
                     EKSLHNFLKAKLNIETSEMSKDKISEILVSRKANVETVSQFITLTENCEFARYAPSTS
                     SSIQQDFDKAVTILSDLEKQIS"
     gene            complement(4051..4815)
                     /locus_tag="PK218_01610"
     CDS             complement(4051..4815)
                     /locus_tag="PK218_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW97238.1"
                     /translation="MKRILTYTLLLLSFLTFAQKKEKDRNLPDGNEAFSEKNYSNAEA
                     DYRVSQSGFPKKAVASYNLGNSIYRQKQASEAKFQFVKAIKSAKTKPEKHKAYHNLGN
                     CLMTEKNYEGAVEAYKNALRNNPNDEETRYNFALAKKQLENNPPKSEDDKNKNKDKNK
                     DKKEDKKDQDKKDNKDKKDKDKDKNDQKKEGDKNKDQQDKNNDKNGQPKPQPGGISKD
                     RIQNMLDAVNNEEKKIQDKVNQQKVRVNPKKPEKDW"
     gene            complement(4816..5853)
                     /locus_tag="PK218_01615"
     CDS             complement(4816..5853)
                     /locus_tag="PK218_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPW97239.1"
                     /translation="MYELEEKGYFYLLLVIPVLTLVFLYLLYWKRKKQREFGDLNLIE
                     KLSPEKSTFKPIFKFIVVILGLVCLIIGLINPKMGTKLETVKREGIDIIFAIDVSKSM
                     LAEDVAPNRLEKSKQLVSQIINKLGSDRIGIIAYSGSAFPVLPITTDYSVAKMFLQSM
                     NPGIVSTQGTSIDEAINLATTFVDKKDKTNKLLIIISDGEDHSENAVTAAEEAKKVGL
                     KIITVGVGTEKGGTIPLKRNGVVESFQRDQNNEVVITKRNAEVLSLIAKSTKGGYVDG
                     NSTKDVLTYVKNALENIEKTEFESTQMADFKSQFQWFLGFGFFLLFLDMFLLEKKTKW
                     VRKMNLFNEKE"
     gene            complement(5970..6968)
                     /locus_tag="PK218_01620"
     CDS             complement(5970..6968)
                     /locus_tag="PK218_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPW97240.1"
                     /translation="MKDVTFLNPEFFWLFLLLPVAMVWLFWKRKDSTATLKMSSLSGF
                     STTWKTKLKPYLVVFRILALSSLIIAMARPRTVDISSQTKSTKGVDIVMAIDVSGSML
                     AKDLKPNRMEALKKVASNFVEGRPNDRIGIVLYAAEAYTKTPVTSDKAIVEDAIRNIE
                     YSQVLQDGTGIGMGLTTAINRLKDSKAKSKVIILMTDGVNNSGFIEPETASKIAKDFG
                     IKVYTIGIGTNGMAEFPYAYAPNGKGFLFKMMPVEIDVNLLQTIAKNTGGKYFRATSN
                     SKLEEIYKEINKLETTEIQELKFYDYDEKYRPFVWIAGILILIEFGLRNTTFRGFI"
     gene            complement(6968..8602)
                     /locus_tag="PK218_01625"
     CDS             complement(6968..8602)
                     /locus_tag="PK218_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97241.1"
                     /translation="MKIKIILFFLLLSNVLFAQKVTTSIDSTKKKIGSEFKLTLKTEV
                     DTTAKVTFPKNKTFGRLEVLESYKIDTVKKGSRYELIKKYGLTQFDSGKYLIPSIPVL
                     INGKKVFSDSIQIEIADVKVDTLKQKMYDIKNIIQVDKPIGNWWIYVLILIAIGGIGY
                     LVYYFIKKKQAEPKPEIVIFTSPIEKATTLLLQLETKELWQKGEIKDYYSELTDIARN
                     YIEEEIKIPAMESTTSELIEGLRNAARQKKLKLSNETVENLEKVLKQADLVKFAKSIP
                     VQFEIEEDKKRISSTIVTIHKSIPEIVEEDDELALWNEQQKEKARIEKLKKQKRVRII
                     TTIGIVLGIIVLSLGTLVYIKGFDYLKDNIIGHPTKDLVEGEWIYSDYGNPSVRIETP
                     KVLKRIDAEKVLPKQTMALLKEMQIFQYGSAIDDFYIAVSTTSFKQETQIDLTQAAEG
                     SIKVFEAQGGQNILVKTEDFDTQKGIKGKKAYGTMTMMDKIKGKSTKMYYEILVFSQN
                     NGLQQITVMHREDDKYGKEIMNRVLNSVELQNYSQP"
     gene            complement(8605..9474)
                     /locus_tag="PK218_01630"
     CDS             complement(8605..9474)
                     /locus_tag="PK218_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPW97242.1"
                     /translation="METKDLLKKVRKIEIKTRRLSDHIFSGEYHTSFKGRGMTFSEVR
                     QYQYGDDIRAIDWNVTARYNEAHVKVFEEERELTMMLMVDISGSESFGTKNQLKSEIV
                     TEIAATMAFSATQNNDKIGLILFSDQIELYIPPKKGKSHVLRIIRELIEFQPKSRKTE
                     LSQALKFLSATQKKKAIVFLISDFIVEDDYEKTLKIAGKKHDITGIRVYDAREEKMLN
                     IGVVEMMDAESGKWITVDTTSKKARMNYEKNYHEKVNYFKDIFSKCGSGTVNTRVDES
                     YVTKLLGYFKSRS"
     gene            complement(9562..10566)
                     /locus_tag="PK218_01635"
     CDS             complement(9562..10566)
                     /locus_tag="PK218_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPW97243.1"
                     /translation="MEENSAALDIRAINEKIERESAFIDLLIMEMNKVIVGQKHMVER
                     LLIGLLGQGHILLEGVPGLAKTLAINTLSQAVHGSFSRIQFTPDLLPADVVGTMIYNI
                     KQNEFSIKKGPIFANFVLADEINRAPAKVQSALLEAMQEKQVTIGDTTFKLERPFLVL
                     ATQNPIEQEGTYPLPEAQVDRFMLKTVIDYPKMDEERLVIRQNLKGSYEKVNQVVSVD
                     QILRAQEAVREVYMDEKIEKYILDIIFATRYPEKYKLESLKPLISFGSSPRGSINLAN
                     AAKCYAFIKRRGYVIPEDVRAVVHDVLRHRIGITYEAEAENITSVDIINKIVNEIEVP
                     "
     gene            10814..11680
                     /locus_tag="PK218_01640"
     CDS             10814..11680
                     /locus_tag="PK218_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPW97244.1"
                     /translation="MQTKLSPIVSGTMNWGIWDKNLSTKEMVHLINICVENKITTFDH
                     ADIYGNYTTEADFGKALKESKMDRNKIQLISKCGIQHTLGRNNAIKHYDYSKEYITWS
                     VENSLKNLQTDYLDVLLLHRPSPLMIADEIAEAVEQLKEKGKIIDFGLSNFTSSQTEL
                     IRQKTEVNYNQIQFSATHHEAMLDGSLDYMQTHNIRPMSWNPLGTVFREDIEQTRRLK
                     KLLVQLVEKYGVGSDTLLLAWILKHPAKIIPVAGTVNIARIQQLMKAVELNFDTLDWF
                     AIWTESMGNKVP"
     gene            11745..12893
                     /locus_tag="PK218_01645"
     CDS             11745..12893
                     /locus_tag="PK218_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW97245.1"
                     /translation="MINKRLLIKNLLAHNDESTFYDKKRQLNLHSKEGKAKFLKHICA
                     LSNSNPSNNSYIVVGVEDENNEISGDDFFDDSRIQNLVNAYLENPPKILYENVPFPNL
                     PKDRVIGLVTIKPNHKTSFFKKNIYTITANSVFIRIGSNSMPTEDKIPYSKSNIETVI
                     SIENNSRNSIAHTLDGVMDFMNIRHGDMTSKYKVFKELFVVCWAGIPKRIRETNYLSR
                     VDIELINEQIKLFYSTLDVVTISYDENTFTITEHVQLGLNDKTSYYPLEKVTIHFYDN
                     GYYKMETKMLFEPPKYNLKMLHHIYNSNLTLLSKLQKKIELTERENKDLFHFSHTLMI
                     CYLNGFGDAKQKIIDAKPFLKAHSNPLVYLNFKDALRILRKMKYNEND"
     gene            12886..13614
                     /locus_tag="PK218_01650"
     CDS             12886..13614
                     /locus_tag="PK218_01650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPW97246.1"
                     /translation="MTRTLVIGDIHGGLRALHQIMERAKVTANDKLIFLGDYVDGWSE
                     SPQVIEYLIALGQKQDCIFINGNHDELLLEWLSESKDNLDWYKHGGESTVLSYSLFPS
                     ETKEKHIEFLQSLKSHYIDDKNRLFIHAGFTNLNGIDYEYFPKLFSWDRTLWEMALCL
                     DDRIPIESELYPSRLKKYHEIYIGHTPTTRINETKPLQKANVWNVDTGAAFKGKLTIL
                     DIDTKEFWQSEPVFELYPIEKGRN"
     gene            13654..14151
                     /locus_tag="PK218_01655"
     CDS             13654..14151
                     /locus_tag="PK218_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97247.1"
                     /translation="MKNILTLVAFLSISLLHAQAFKGKGDIKGQIGLTLQNGGTGIGI
                     SSDFGIGENMSFGFVAGYMLNADKIAEVKPRFEDRADFKVRFNANIGNVLKLDDNMDV
                     YPGLSLGLRNFGGHVGFRYFFTDGFGMFAEGSAPLARYDTDVNGFEHYNNQFVFIIGT
                     SFKLD"
     gene            14152..14727
                     /locus_tag="PK218_01660"
     CDS             14152..14727
                     /locus_tag="PK218_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HPW97248.1"
                     /translation="MSIVTEKRVKERSGSVCEISGSDENLVLYMVEPKTEDIPENCIL
                     ITQKLKDQIENPETTNANDWRGLSDSMWNENLPVQIVSWRMHARLKNMDMLEMMYLDE
                     DALEWAKATGEADDDEGKIIHKDSNGNILNDGDSVVLIKDLDVKGANFTAKRGAAVHN
                     IKLVWDNAEQIEGRVENQHIVILTQYVKKTK"
     gene            complement(14809..16362)
                     /locus_tag="PK218_01665"
     CDS             complement(14809..16362)
                     /locus_tag="PK218_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPW97249.1"
                     /translation="MSTIAQQFGMTEALEILGVKAINEGTSTGNEWFSSGEIIESYSP
                     VDGQLIGKVKATTKEDYEKVMQAATSAFLTFRAMPAPQRGEIVRQFGNKLRELKEPLG
                     KLVSYEMGKSLQEGYGEVQEMIDICDFAVGLSRQLNGQTIPSERPGHVMREQWHSLGV
                     VGIISAFNFPVAVWSWNTALAWICGDVCVWKASEKAPLCSIACQNIIAGILKENNLPE
                     GISCIINGDYKVGEMMTTDTRIPLISATGSTRMGRIVGAKVAERFGKSLLELGGNNAI
                     IITPTADLKVVVPGAVFGAVGTCGQRCTSTRRLIIHESVYDKVRDAIVGAYKQLTIGN
                     PLDQKNHIGPLIDKDAVNTYLAAIEKAKAEGGKVLVEGGVLSGEGYESGCYVKPAIIE
                     AENHFEIVQHETFAPVLYLMKYSGEVENAIELQNGVAQGLSSAIMTNEMKEAEKFLSF
                     AGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKIYMRRQTNTVNYSDQLP
                     LAQGIKFDL"
     gene            complement(16552..17085)
                     /locus_tag="PK218_01670"
     CDS             complement(16552..17085)
                     /locus_tag="PK218_01670"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HPW97250.1"
                     /translation="MSIQKPFNLNQWIDENRHLLQPPVGNKNVYVDSGDYIVMIVAGP
                     NARKDYHYNETEELFYQLEGEITVYVQDNGKKKAMNLSAGDMFLLPAKVPHSPVRKEN
                     SIGLVIERKRVGHDIPDGLLWFCDNCNHKLYEVYFELNDIEIDFLKHFEHFYNSEELR
                     TCTNCGTVMEADVRFVK"
     gene            complement(17148..18263)
                     /locus_tag="PK218_01675"
     CDS             complement(17148..18263)
                     /locus_tag="PK218_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPW97251.1"
                     /translation="MRIKKLIAVFFVLFAIFSSNGQGKKYNIHTVAFYNFENLFDTIN
                     GPNNDEEWLPNGAMNWTGKKYKQKLHNLARVINEIGSTENPSNSPTLLGCSEIENRGV
                     LEDLVKEPLLINRDYGIVHFDSPDKRGIDVGLLYQKKHFKPTSYKNIPLIIYSNQEGK
                     EDKKDKKDKEEATDDVTTASKTDNRVYTRDQLLVTGFLDGEEINIIVNHWPSRSGGEK
                     KSSPFREAAGRLNRKIMDSLFAINPNAKIITMGDLNDGTYNKSVKEGIGAKRKKEELK
                     QPSDIYNPFEEMFYKGNASLFWRDSGDIFDQIMVSETFVRKDYSTFCYWKAGIFNKPY
                     MIQTTGQYKGYPLRNSSSEPGFSDHFPVYIYLLKEIK"
     gene            18403..21207
                     /locus_tag="PK218_01680"
     CDS             18403..21207
                     /locus_tag="PK218_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW97252.1"
                     /translation="MKKLVFSTLFVLQTFLIFAQASSGVTGKVIDSKSQKPLQNVVAS
                     IQNTNLTALTNALGVFTIKDISDGSQLLRISSVGFKDQLLAVEITKGEIIDLGIVVLE
                     EDITTEQQLSLVTLTENDLGDDNSGSESTSGLLQASRDSYQQAAAFNWGQARFRIRGL
                     DNEYGTTMINGVVMNKILDGRPQWSNWGGLNDATRNQEFTMGSAPSDYTFGGILGTQE
                     INTRASTYRKGTRVTFSGTNTNYSWRSMGTYASGMNTDGWAFVVSGSRRWAQEGYFEG
                     TDYSANSLFASVEKKFNDKHSLNFTSIYAQNSRGKSSPNTKEVNDLKGIKYNSYWGWQ
                     DGRKRNSRDKDIEEPINMLSHYWKISDKTSLNTNVAYQLGYIGNSRIDNQNSKNPDPS
                     YYRDLPSYYLNNPDSYVTIGGVTTNPEADAARQKFINDGQLDWIAMYAANKRSANAGK
                     SVYVLYQDRTDDRQFSANSILFSNLSDNISLNAGGTYRKLKSHNYQKLEDLLGGQFFL
                     DVDPFYSGDQTQSDLNNPNRKVVVGDEYGYNYNLNADVFDAFTQFKFNYKKFDFYLAQ
                     SFSKSEYQREGLYRNGIYANSSLGYSNKVSFENFGFKGGLTYKITGQHYLNFNGAYMT
                     KAPGMRNVFSNARINNNITPDLQSENVFSVDGSYIIRTPKLKMRLTAFYTKISKATEL
                     SFFYGEGLFDEATVGVGNEDSFISEIITGIDKKNIGAEFGFEYQLTSTIKATVNASYG
                     QYTYDNNPNVYLNDDAQASLTNTSPITNFGSTYLKDYRMPGMPQQAYSFGLEYRDPKF
                     WWISANINHLDDNYLDVSAVRRTTNFVDDNGIGYDGATIDNVRKVLKQEKFDAFQLLN
                     LAGGKSWRISSKNRNTVGFFASINNVLDVTYKTGGFEQSRKATYPDYVADNSNGTPSF
                     GPKYFYGYGRTYFLNVYINL"
     gene            21219..21860
                     /locus_tag="PK218_01685"
     CDS             21219..21860
                     /locus_tag="PK218_01685"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPW97253.1"
                     /translation="MKNIFKVTLFFVFSLTLLSSCSKEDDYSIPPYKPLFFNEGFEEI
                     PRANTGANQFITLEGWSNVSLNGGARKWEARYYSADGQYAQLQAFQSNETNMDTWLIS
                     PALNFDLTENEGLIFSYKQAFYNGQPLSVLISTNYDGSNTAQAINNATWTDMNVVLPD
                     FLTTGYPSSFSKSEIIDLSEFDGTVYIAFRYKGSSSGISTTFQLDDIKLFENK"
     gene            21866..23170
                     /locus_tag="PK218_01690"
     CDS             21866..23170
                     /locus_tag="PK218_01690"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HPW97254.1"
                     /translation="MKNLLKLLTITLFAALTSCVNSDDYGTPDLSGECSNLTATKNTP
                     DILAMATSNIQQFGPAYDNDIIEAIVTSSDEGGNFYKSISLMSLDGTKGFSMPLDDYN
                     LYTKYEPGSKVFIKLKGNFIQYNNTTSTVEIGSNYQGKVGRISGIFYKDVVKRSCEDN
                     VGEDNIVNHITIAQANNNSYINKLVEFDNVQFTDASIGHTYFDKDLNPVSTFTATNHL
                     IRDADGNKIIVRVSQYANFSGNQVPSGNGKIRGVLTKYNGDYQFMIRTEADVKLTNPR
                     VLPFYLETFQTTFDDWTKYNVTGAQVWSLSDVGNPGKCADMNGFASGAQTNEDWLISP
                     SLDLTSVSSAILSYQTAKNFTGNAIQVLISTNYVGSAAPSTATWTPLGGSIATASNFT
                     WKDSGDVSLASYLNNSNVRIAFKYTSNTSGAAQWRVDNVRID"
     gene            23272..25173
                     /locus_tag="PK218_01695"
     CDS             23272..25173
                     /locus_tag="PK218_01695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPW97255.1"
                     /translation="MKKTALLILSFMALQATAQQVLTPEILWKLNQVSVLGITKDGKS
                     IIYKVKTPTVEENKFSSKTYSLPIAGGNPTEISDTKNLIVDKNVSPDGKYVLSSNEVK
                     IDKVLGKDYYPELEKSDVQIYDALDYRHWDTWNEGLHNHVFFGENNANTKPTDIMAKE
                     PFDCPQKPFGGDEDYIWSPTSKSIVYVSKKLTGTQYAISTNTDLYEYNIETKTTKNLT
                     EANKGYDTNPAFSPNGQLTWLQMKRDGYEADKNDIIVRIKDNYDLNLTAAWDGSVDSF
                     QWSADGKKIYFTAAVNGTKQLFEVNYPGLTKIAVRVTQITDGDFDVTGLVGFSGENAI
                     LTRTDMNHAAEIFSYNTKKKTWTQLSNTNQETYSKLSLPKYERRYVTTTDGKKMLVWV
                     ILPPNFDKTKKYPTLLYCQGGPQSPLTQFYSFRWNFSLMASQGYIVVAPNRRGMYGHG
                     QAWNEQISGDWGGQVMNDYLSAIDDVSKENYVDKSRLGCVGASYGGYSVFMLAGIHNN
                     RFKTFIAHDGVYNLVSMYGTTEEVFFDNWEHGGAYWEQNPIAKKTYTTFNPITHIDKW
                     NTPILIIQGGLDFRVPIGQGQEAFQAAQLKGIKSRLLYFPQENHWVLKPQNAIVWQRE
                     FYKWLKETL"
     gene            25683..26012
                     /locus_tag="PK218_01700"
     CDS             25683..26012
                     /locus_tag="PK218_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW97256.1"
                     /translation="MKTRQIERPYTYLVKQGISPHTAHKLLHSQTRTVRLDHIERLCE
                     ILHCDPNDLIRFKPNSIQKQQTNHPLQKLIQKETDFDWQNSLKTMPLEKLKELSKLIT
                     DAENKEE"
     gene            complement(26057..27400)
                     /gene="accC"
                     /locus_tag="PK218_01705"
     CDS             complement(26057..27400)
                     /gene="accC"
                     /locus_tag="PK218_01705"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPW97257.1"
                     /translation="MFKKILIANRGEIALRVIRTCKEMGIKTVAVYSTADAESLHVKF
                     ADEAVCIGPPPSNLSYLKMSNIIAAAEITNADAIHPGYGFLSENAKFSKICQEHGIKF
                     IGASPDMIDKMGDKATAKATMKAAGVPCVPGSDGILEDFEEAKKVAKATGYPVMMKAT
                     AGGGGKGMRAVWKEEDLEKAWESARQESAAAFGNDGMYMEKLIEEPRHIEIQIVGDSY
                     GKACHLSERDCSVQRRHQKLTEETPSPFMTDELRHKMGEAAVKAAEYISYEGAGTVEF
                     LVDKHRNFYFMEMNTRIQVEHPITEQVIDYDLIREQILVAAGVPISGKNYLPQLHSIE
                     CRINAEDPYNDFRPSPGKITVLHAPGGHGVRLDTHVYAGYTIPPNYDSMIAKLITTAQ
                     TRGEAINKMKRALDEFVIEGIKTTIPFHRQLMDDPAYVEGNYTTAFMDTFKMDEL"
     gene            complement(27494..27979)
                     /gene="accB"
                     /locus_tag="PK218_01710"
     CDS             complement(27494..27979)
                     /gene="accB"
                     /locus_tag="PK218_01710"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HPW97258.1"
                     /translation="MDLKEIQNLIKFVSNTGVAEVKLETGDVKITIRTTLEGHAPEVT
                     YLQAPMQQAMQTAPIAAPAPAAVPATPVAVAPAEENSKYVTIKSPMIGTFYRKPAPDK
                     PMFVEVGSSIGKGDVLCVVEAMKLFNEIEAEISGKIVKILVDDMSPVEFDQPLFLVDP
                     S"
     gene            complement(28006..29004)
                     /locus_tag="PK218_01715"
     CDS             complement(28006..29004)
                     /locus_tag="PK218_01715"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPW97259.1"
                     /translation="MNKITAAITAVGSYVPEFVLSNQVLETLVETNDEWITSRTGIKE
                     RRLLKEKGKGTSYLAIKAAQDLISKANINPEEIDLVILGTTTPDMPVASTSVYVASQI
                     GAVNAFAYDLQAACSSFLFGMSTAAAYIESGRYKKVLLIGADKMSSIIDYTDRATCII
                     FGDGAGAVLFEPNYEGLGLQDELLRSDGIGREYLKIDAGGSILPPSIETVENRQHFVF
                     QDGKTVFKYAVSGMADVSEKIMQRNNLTKDNVNWLIAHQANKRIIDATANRMGLEEEK
                     VLVNIQKYGNTTSATLPLLLSDFESKFKKGDNLIFAAFGGGFTWGSIYLKWAYNKQ"
     gene            complement(29157..29351)
                     /gene="rpmF"
                     /locus_tag="PK218_01720"
     CDS             complement(29157..29351)
                     /gene="rpmF"
                     /locus_tag="PK218_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPW97260.1"
                     /translation="MAHPKRKISKTRRDKRRTHYKATAATIATCPVTGEAHLYHRAYW
                     HEGKMYYRGQVVIDKSVAEA"
     gene            complement(29361..29903)
                     /locus_tag="PK218_01725"
     CDS             complement(29361..29903)
                     /locus_tag="PK218_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPW97261.1"
                     /translation="MKSTNEYLIPFIGLKLGKHQFEYTIDKKFFDEFSFDEFESCEIK
                     AAVVLEKKATMLEIMFKHNGTVNVPCDLTGELFDLPIKGKLKLIVQFGDVFNNDNDEL
                     LILPHGEHQIDLSQYIYEMIALSIPLKRIHPGVKDGTLQSEALDKLKDLQVNEIKEKD
                     KKEDDIDPRWDELKKLLTDK"
     gene            complement(29989..31035)
                     /gene="pdxA"
                     /locus_tag="PK218_01730"
     CDS             complement(29989..31035)
                     /gene="pdxA"
                     /locus_tag="PK218_01730"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPW97262.1"
                     /translation="MKKAENIIVGISIGDLNGIGSEVILKTFEDTRMLELCTPVIFAN
                     VKILSFIKKHLNLTAQLHGIDKIDQIVIGKINVLNIWREGVDLNFGVNDVKVGEYAIK
                     SFVAATKALKENKIDVLVTAPINKYNIQSDEFKFPGHTDYLNQELEGNALMFMIQDNL
                     RVGLFTDHVPVNEVANHLNEKLILQKIETIKKSLMQDFGINKPKIAVLALNPHAGDNG
                     VIGKEDDELLKPIIKKIFEKKTLVFGPYAADGFFGSNQYQKYDAVIATYHDQGLIPFK
                     TLSFGNGVNYTAGLNKIRTSPDHGTAYEIAGKGIADYNSFKEAVYAAIDIYNSRNEYE
                     EITSNPLKTKEKQL"
     gene            31096..31701
                     /locus_tag="PK218_01735"
     CDS             31096..31701
                     /locus_tag="PK218_01735"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPW97263.1"
                     /translation="MFTGIIETLGVIKEIRKEEDNLQITISSSITNELKIDQSVAHNG
                     VCLTVIAVENDCYTVTAIKETISKTNLSDWRVEDIVNLERAMRLGDRLDGHIVQGHVD
                     QVGLCKSIVETNGSWCFTFEYDKYLQNITIEKGSITINGVSLTVVNSKENEFSVAIIP
                     YTFENTNFKNFKVASRVNLEFDVIGKYVSKLYSSKNEKASN"
     gene            complement(31698..31916)
                     /locus_tag="PK218_01740"
     CDS             complement(31698..31916)
                     /locus_tag="PK218_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97264.1"
                     /translation="MRIVPNRLIALILAMALPCGVVFGASNPPPPSTPPPPPGLPIDG
                     SLYVLLVISVVFGIYKVYKHHNIKKASN"
     gene            complement(32138..32211)
                     /locus_tag="PK218_01745"
     tRNA            complement(32138..32211)
                     /locus_tag="PK218_01745"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32175..32177),aa:Ile,seq:cat)
     gene            complement(32287..32688)
                     /gene="mce"
                     /locus_tag="PK218_01750"
     CDS             complement(32287..32688)
                     /gene="mce"
                     /locus_tag="PK218_01750"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPW97265.1"
                     /translation="MRKIEHIGIAVKNIEDSNHLFEKLFGNPPYKEEIVESEGVKTSF
                     FMNGPNKIELLEATNLESPIAKFLEKKGEGIHHIAFDVENIFSEIDRLKAEGFVVLND
                     SPKKGADNKLVVFLHPKSTNGVLIELCQEIG"
     gene            32789..33181
                     /gene="rbfA"
                     /locus_tag="PK218_01755"
     CDS             32789..33181
                     /gene="rbfA"
                     /locus_tag="PK218_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPW97266.1"
                     /translation="METNRQKKIGSVLQKDLVDILQGEVRKNGISNLVISVSKVTVTT
                     DLSIAKVYLSVFPQDKAKDLLEAVKTNAPLIKHDLSQRVRLQLRKVPNLAFYIDDSLD
                     YIEKIDNALSGKENPIDNRDLLDKRKKF"
     gene            33183..34382
                     /locus_tag="PK218_01760"
     CDS             33183..34382
                     /locus_tag="PK218_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW97267.1"
                     /translation="MNFSLYIAKRYLFSKSNNNAINIITGIASLGIIVGTLALFVVMS
                     VFSGLKVFSLSFSNDFDPDLKVFPTKGKTINISNSQENQLKKVKDIAVYSKITEERVL
                     FYFNGKEIVTYLKGVDANFTKTNVVSKNLFNGEWLSPKTPEVVVGYGISEKLSLGLFD
                     FNNPFEVYVPRKGKAIIESEAEAFNRSKLVPIGIYAISEDLDSKYVFCDLELAQKLLE
                     YKPNEVSAIEIKIDSLADENTVIEKLNTIFKNKVIIKNRSEQNSTLYKMLNTENIAVY
                     LIFTLVIIIALFNLVGALIMMILDKKGNLKTLFNLGAEIKELKKIFLFQGSLLSVFGG
                     IIGLVLGIIIVLIQQKFKVVMITSTLAYPVVFSIQNVLIVFSTIVLLGVLSSWIASSR
                     INAKMLD"
     gene            complement(34379..35371)
                     /gene="dusB"
                     /locus_tag="PK218_01765"
     CDS             complement(34379..35371)
                     /gene="dusB"
                     /locus_tag="PK218_01765"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPW97268.1"
                     /translation="MPKIGNILLPDFPLLLAPMEDVSDPPFRRLCKLHGADLMYSEFI
                     SSEGLIRDAMKSKMKLDIFDYERPVGIQIFGGDEEAMEMSSRIVDAVQPDLVDINFGC
                     PVKKVVCKGAGAGVLKDVDLMVRLTKAVIKGTNLPVTVKTRLGWDESSINIDEVAERL
                     QDIGVQALTVHARTRAQMYKGHSDWSHIARVKNNPRITMPIFGNGDIDSPEKALEYKN
                     KYGLDGMMIGRAAIGYPWIFNEIKHFFETGEHLAQPTMQDRIEAARNHLTWSMEWKGE
                     RVGIVEMRRHYTNYFKGISNFKEHRLKLVTLESSEELFKALNQIQEVYADYQFS"
     gene            35508..37304
                     /gene="lepA"
                     /locus_tag="PK218_01770"
     CDS             35508..37304
                     /gene="lepA"
                     /locus_tag="PK218_01770"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPW97269.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLGATQTVTAREEKAQLLDNM
                     DLERERGITIKSHAIQMEYEYKGEKYILNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     AAQSIQAQTISNLYLALENDLEIIPVLNKVDLPSANPEEVSDDIIDLLGCKLEDIIHA
                     SGKTGFGVENILAAIIEKVPAPKGNVDEPLQALIFDSHYNPFRGIEVIFRVKNGEIKK
                     GQKIKFMATDNEYFADEVGTLKLNQVPKNVISTGDVGYLISGIKEAKEVKVGDTITDA
                     KTPTTNMITGFEDVKPMVFAGIYPVDTEDYEDLRSSMEKLQLNDASLVFTPESSAALG
                     FGFRCGFLGMLHLEIIQERLEREYDMTVITTVPNVSYLAYTKKEPEVGFVVNNPSDLP
                     EPSRLDRVEEPFIKATIITKSDFVGNVMSLCIEKRGVITNQTYLTTERVELNFDMPLA
                     EIVFDFYDRLKTVSKGYASFDYSPIGMRTSKLVKLDVLLNAQTVDALSALIHEDNAYN
                     IGKKMTEKLRELIPRQQFDIPIQAAIGAKIIARETIKALRKDVTAKCYGGDISRKRKL
                     LEKQKKGKKRMRQVGNVEIPQEAFMAVLKLND"
     gene            complement(37809..41750)
                     /locus_tag="PK218_01775"
     CDS             complement(37809..41750)
                     /locus_tag="PK218_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HPW97270.1"
                     /translation="MKYKLLSVALMAFASFTFAQSKSSLWNATNKMNDKVALNERLQL
                     PENQVFELNLNGLRTSLTTAPKRTTKGQSENILLIPTASGSLERYKIYENSIMEPGLA
                     ERYPEIKSYIGIGVDNPSATVYFSVSPLGFKGMVLSADKAAEFIEPITTDLTTYTVYR
                     KSDKAASLTPFECSVIDVIKPTEASATMLRPNADDATMRTFRLAMSVTGEYTSYFGGT
                     KALALAAINNTMTRVNGIFEKDFGARMVLIANTDAVIYTNASTDPYSAASTGAGGAWN
                     TELQNTLTSVIGNAAYDIGHLFGASGGGGNAGCIGCICVDDTSSTTDKNKGSGFTSPA
                     DGIPAGDNFDVDYVAHEMGHQFGANHTFTHSNEGTGVQMEPGSGSTIMGYAGITTKDV
                     QPHSDAYFHAISIQQVTNNLKTKTCSVNTATGNSVPTANAGLDYTVPKSTPFMLTGTA
                     TDANGDALTYNWEQMDSQTTSAVPSATKTSGVNFRSYNSSSSPTRYFPTMSSVLSGAL
                     YTAGSELNVEYLPSVARTLNFRLTVRDNRAGGPANNSDDMKVTVTTSAGPFTISSPNT
                     AVSYVGGTTQTVTWAVAGTTANGVNCANVDVLLSTDGGNNWTTILAATPNDGTQAVTI
                     PNTPSTSCRIMVKGTNHIFFDVSNTNFTITAGSAPDTQAPTAPTLAASGTTQTTTNLS
                     WSGATDNVGVTGYDVYQGAALLGSTATSTYAVTGLMASTTYTFTVKAKDAAGNISVAS
                     NTVSVTTSAPAGDTQAPTAPTLAASGTTQTTTNLSWSGATDNVGVTGYDVYQGAALLG
                     STASTTYAVTGLSASTAYSFTVRAKDAAGNVSVASNTVSVTTLSAGITYCTSQGNSIN
                     DEFIGRVQIGSIDNTSGGISGYVDYTSLSTNLTQGAAATITITPTWTGSTYSEGYAVF
                     IDYNQDGDFDDAGETVWSKATSTTTPAVGTFSVPTGATSGATRMRVSMKYNGTPTACE
                     TFSYGQVEDYTVNIVVAAGDTTAPSTPTNLVASGTTQTTTTLTWNASSDNVAVTGYEV
                     FLNGSSIGTVTTTSSNITGLTASTAYSFTVRSFDAAGNNSAMSNVANVTTLSNSVSYC
                     TSQGNSTADEKIGKVVFGAINNTSTGTTGYENFTALSTTVVRGSANTITITPSWTSTK
                     YNEGYAVFIDYNQDGDFVDAGETVWTKTASKTTPVSGSFTIPASALLGSTRMRVSMKY
                     NGIPTSCESFSYGQVEDYTINISASGRMEDISSTALTFTLYPNPVKGNLLNIANIEGD
                     ATYRIFNLMGQELESGNIEDNAVLVGNLKTGAYLIEITSRNQTATKRFIKE"
     gene            41972..43090
                     /locus_tag="PK218_01780"
     CDS             41972..43090
                     /locus_tag="PK218_01780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW97271.1"
                     /translation="MNLSYWEQKTWFSDVDYTIVGSGIVGLHCALTLREKYPNSKILI
                     LEKGMLPQGASTKNAGFACFGSISEIIDDLKSHTEEEVVQLIQKRWNGLQLLRKNLGD
                     AAIDFKPFGGYELFLKDAESSYNECLQKIPFINEVLKPFFKNDVFTKEVDRFNFKEIK
                     EYLIFNPFEAQIDTGKMMQTLLQKAISNNILILNQVMVNSYSENTNGVEVALNDFSFT
                     TKKLLFATNGFAQKLTNNHVKPARAQVLITEPIPNLDIKGTFHLDKGYYYFRNIDDRI
                     LLGGGRNIDFEGETTTELAQTEKIQNRLEDLLKEVILPDTNFKIAHRWSGIMGIGSSK
                     NPIVEHLSEHVYCGVRLGGMGVAIGSLIGKELAELVQA"
     gene            43087..43767
                     /gene="mtgA"
                     /locus_tag="PK218_01785"
     CDS             43087..43767
                     /gene="mtgA"
                     /locus_tag="PK218_01785"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPW97272.1"
                     /translation="MIKKILNFIKKTLLWFFAISILSVIIFKWVPVPITPLMVIRVVE
                     SKFEDSETIFSHDWEPLENISPNLQKAVIASEDGNFLKHSGFDFDAMQKAFKNNKKGK
                     RIKGGSTISQQTAKNVFLWQGRSYLRKGLEAYFTVLIELIWGKERIMEVYLNSIEMGN
                     GVYGAKEAARHWYSTTAKDLTPQQAAGIAAILPNPRKFKASNSSSYINRRKAKIQRVM
                     RQIGKIEY"
     gene            complement(43889..44779)
                     /locus_tag="PK218_01790"
     CDS             complement(43889..44779)
                     /locus_tag="PK218_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPW97273.1"
                     /translation="MKKILLSAVFLFAVNGVFAQVNEKELLKKNEEAEAKLKEEKPMG
                     WTKKGMFTVLANQASFNNWLAGGQSSISGNIGINYDFNYKSEQWNWDNKIIAAYGLTK
                     LKDQDLQKSDDRLVFNSLAGKKAGGYWFYSLFFNFQTQFDSGFDLTSGSKTSHFFSPA
                     YFQFGPGMMWKKSDNLKVNIAPATSKLILVHSHFTDLGPSFGVEQGKSSRFEFGAAIN
                     GYYKFNLMENVSVENILNLYSNYIEDPQNVDIDYTLNIVMKINKYLSTNFAFQTIYDD
                     NAFAGFQTRQVIGVGVNYGF"
     gene            complement(44870..45376)
                     /locus_tag="PK218_01795"
     CDS             complement(44870..45376)
                     /locus_tag="PK218_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HPW97274.1"
                     /translation="MDEIVNKVANSVLEVFDLEDYYPIGIRTQIDISQWLFEGFLLKE
                     KDFREALKNHDWSQYQNHYVAIHCKTDAIIPAWASILVTTYAASFAKKVVLGTINDLN
                     TVLYQEILNQLDYSKYQDKPLILKGCSKKPVPESAYIFAVQHLQKVAKSIMYGEACSA
                     VPLFKKQK"
     gene            complement(45413..45736)
                     /locus_tag="PK218_01800"
     CDS             complement(45413..45736)
                     /locus_tag="PK218_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HPW97275.1"
                     /translation="MEEFKDDINLGENVVKVLKSIYDPEIPVDIYELGLIYDVMINED
                     NEVKVLMTLTSPNCPVAETLPREVEEKIQKIETVKSCEVEITFDPPWSKDLMSEEAKL
                     ELGML"
     gene            complement(45773..46186)
                     /locus_tag="PK218_01805"
                     /pseudo
     CDS             complement(45773..46186)
                     /locus_tag="PK218_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
     gene            complement(46232..46651)
                     /locus_tag="PK218_01810"
     CDS             complement(46232..46651)
                     /locus_tag="PK218_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HPW97276.1"
                     /translation="MTIQEIQNEIVNEFSMFDDWMQRYEYIIDLGKALPLIEEKYKTE
                     ENIIKGCQSKVWVHGEEENGKIVFTADSDAILTKGIIAILIRAFSNQKPEAILEANTD
                     FIDEIGLKEHLSPTRANGLVSMIKQLKMYALAYNAKN"
     gene            complement(46660..47100)
                     /locus_tag="PK218_01815"
     CDS             complement(46660..47100)
                     /locus_tag="PK218_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97277.1"
                     /translation="MKTITMILLTLLMSKGCSNDAKNDISKAVLQYDAHTRGYHLKVV
                     ISNQIATISQARIPDSPSEQIKISDADWKELVGCFEKINLEELPNLKDPSQKRFYDGA
                     AIAELKVRYQDKNYETKAFDHNNPPAEIQQLVDKIVSLAKPLEE"
     gene            complement(47113..48327)
                     /locus_tag="PK218_01820"
     CDS             complement(47113..48327)
                     /locus_tag="PK218_01820"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPW97278.1"
                     /translation="MIDIQKIRADFPILSQKVNGKPLVYFDNGATSQKPKVVIEAISK
                     YYEEINANIHRGVHTLSQLATDAYEVSRGKIQAHINAKFAHEVIFTSGTTHGINAVAN
                     GFASLLNSDDEVLVSALEHHSNIVPWQMLCEKTGATLKVIPMNENGELILSEYEKLLS
                     NKTKIVTVNHISNALGTINPIEFMIKKAHDVGAAILIDGAQAVPHLKPDVQALDCDFY
                     VFSGHKICGPTGVGILYGKEEWLRKLPPYQGGGEMIATVTFEKTTYADLPHKFEAGTP
                     NIAGGIVLGTAIDYLNQIGFDNIANYEHDLLEYGTKKLLEIEGLKIIGTAKEKTSVIS
                     FTIDGIHPYDIGTIIDKLGIAVRTGHHCAQPIMDFYKIPGTIRASFAFYNTKEEIDAL
                     VEAVKKAQMMLS"
     gene            complement(48401..>49095)
                     /locus_tag="PK218_01825"
     CDS             complement(48401..>49095)
                     /locus_tag="PK218_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97279.1"
                     /translation="FFALMGLIGLVGYLVTEEDIIPSYYLPILLFLLSIGLYFFQLQL
                     VKRSTNFIYTNVFQIIKIFSLVLGYASLNYYVVRELSTVLLDLQLQPNQEIPFAPLFY
                     FATFALPVFYISFGLKNKDRFFFWIGLLSLALGFATIRYYYSILPIEYALLLGGSILF
                     AMVYFSIKKVQNNTVGITFKEDKSLNPMAFDLVKAILINANVHSEITTTNESPMEFGG
                     GGFSGGGSGGNF"
BASE COUNT        16044 a         8484 c         7883 g        16684 t
ORIGIN      
        1 aagtttaatg ccgtaaaatt atgccaatcg tcttcaattt ctggaccttc tgaaacatta
       61 aaaccaatgg tagaaaaaat atcaataatt tgatttttta ctaacgaaat cggatgacga
      121 gaaccaatag tcaacggttc gccaggacga gacaaatcgc cataaaaacc tttcacttct
      181 tctttacttt ccaattcttc ctgaatggtt tttacttttt cttcggcaac ggctttcaat
      241 gtgttgatga cttgtccgaa gtctttcttt tggtcgtttg gaatattttt gaattctgta
      301 aaaagttctt tcaacaaacc tttacttccc aaatatttga tacgaaattg ttctaaagct
      361 tctttgtttt tttcattgaa tgctttagct tcttcgatat gttctttaat cttgtctatc
      421 atcgtctttt tgtaaaggtg caaatttagg gaaaagttgg aagttagaag ttagaagttg
      481 gaagtttttc gcagaatgaa cccgaatcta aaatcaaata tcttcaatcg ttactcaatc
      541 acttcttttt ctataaaata atttactatg gcttctttca ttaaaatgga ttgttctccg
      601 gcttttaatg gcggcaattc ttctttgatt ttatagtgtg gccaaccttc tttatcggta
      661 aaattgaatt cataaaaacc ataaggttca agcaaacgac aaatgccaat atgcattagg
      721 tttacttttt catctttggc aaaaacacgc ttgaattgac ctaattcttg tacgccaatt
      781 agataaatga tggcatctaa atccaataaa tcgccatcag caaactgatt ggaaaggatt
      841 tctacaactt tatcccagcg gttttttaat tctatatctc ttgacatatg atttttgatt
      901 aaagaataac gattttagat ttttgaacaa aatcgtatta atttttgcaa atatactact
      961 aaaactcttt tatctttgtt ttgaatttta gatttaaaaa atgagctata ttgacattat
     1021 tcttggcgga ttgttaattt ttggactaat tcgtggaatt aaaaatgggt tgtttgtgga
     1081 attggcatcc ttgctttctt ttttcgttgg aatatattta gcggtaaaat tctcttactt
     1141 ggttggaggt tttattggcg attcaaaatc ggcgaaagtt tttgccttta tcattacgct
     1201 aattgctgtc gtagttggga ttcatttgtt agccaaacta ttttcaaaaa tcgctaactt
     1261 tgtgtttcta ggttggttga atatgcttgg tggtgctgtt ttcgccgttt taaaaaccgc
     1321 aatcttgtta ggaattgttt tgagtttatt ccaaaaagtg aatattgata atgctattat
     1381 ttccaaggaa actcaggaaa gctccatttt tttcaatcca gttatgaaaa catccgaagt
     1441 tttattacca gtgttgacga attggtttaa tgatttgaaa gagaatgtta aagaagaaaa
     1501 ttaattcttc acttccttaa tcgcttcact ttcaatccaa ccagtagttt catctgttaa
     1561 ttgtactttt ttccagttag caatgctttc catgatgaat acttttgttc cttcatggag
     1621 taaaacggca tctgttgctg aaattttcgg ttcacttttc aacgaagtag tttccgcaaa
     1681 aacaattgct ggtttttcgt ttaacattcg gtctcgttca taaattcctg aggaaacgct
     1741 taatgaaatt gcaagtaaaa ccaaaaacat tccagagaaa aataagcgtt tttttactgt
     1801 cgttccagaa aaataatatc ctaaaaagaa cacgaaaaac aagaatgata atccaaccgc
     1861 aatccatgcc caagtgtcat aatgatatgt tgaagtgaaa tcggcgatga ttttatgaaa
     1921 acctactttt gggatgactt tgatatcgtc aattgtcatt tttcgggcaa attcgagatt
     1981 ggttttgatt tcagtatcgt ttggattgag caataatgct ttttcataat tgtaaatcgc
     2041 tggtgcaact tggtgtaatt tgtagtagca atttcccaga ttaaaataga cttccgccga
     2101 ttgttttcct gagtttatga tgttttcata gctttgagtg gcttcagtgt acttttcttt
     2161 agcatacaaa gcattacccg tttcaaaatc ggattgtgcc caaagtgttt gagcaaaaat
     2221 caataggatg taaagtatat ttttcatctt atgaaatctg tttttctaaa tccgacaaaa
     2281 ttgttactgc tttgtcaaaa tcttgttgaa tggaactact ggttgatggt gcataccgag
     2341 caaattcgca gttttcagtc aatgttataa attgtgaaac ggtttcaaca ttcgcttttc
     2401 tggaaactaa aatttctgaa attttatctt tactcatttc tgatgtttcg atatttaatt
     2461 tggctttcaa gaaattatgc aatgattttt ccaatgccac ataaaaaggt tctttgttgt
     2521 tcaattgttt tttggcttcc gataaatatt tttttgccaa agcacttgac tttttaattt
     2581 tatttccaac aacatcattg tctattgctt cttttttctt tctcactaaa accactaatg
     2641 gaatggctaa aaaaggtaaa aacaccaatg aataaaacaa tcctgaacct agaaaatcat
     2701 cttcttccat tggcgacaaa gtagtttttt gtttgatgta ggcaaaagat tttgtttttt
     2761 cgatggtatt tttttctgaa gttttgggtg aattagaagc cgcaatagcg tcatttggac
     2821 catcaagaac attgattaca atttcgggtg attcgatggt tttatatttt ttagtatcca
     2881 aatcaaagta actaaaagtc aatggtttga ttttataatt tcctttaaat tgtggaatta
     2941 aggtatattt gtcactaata cttcctgtca ttccagacaa tggtgtagaa acattttcgt
     3001 tgtgttctgg atcatacatt tctattgatg atggcacaac gggttttggc aaactgaata
     3061 attttaaatt tccagttcca accactttta cggtcaaatc gagcgactcg ccacttttta
     3121 aagttgtttt agatggtgtc actttaaaat caaattttcc aacggcatcg ccaaaatcta
     3181 atggttttcc ttcgtttggc aaggatttta cagtaataac tttacttcct gccgaaactc
     3241 ttttactatc ttcggtcatt aatggctgtc cccaaaagtt tcttctattg gttggtactt
     3301 gacagtcaat atcgagtgaa agtggttcaa tttcgagttt tcctgatttt tgtggatata
     3361 aaacggtttt tcttaaaacg acatatcggt atctttcacc tttaaacatt ccttcttcgg
     3421 cggccaattg tttaatgtcg atgttttggc tccaaaaatc attgtacttt ggtttgctga
     3481 gttcacgcca atttgatatt ccgatgttgt aactaaaata gagcttatag actaccgtta
     3541 ttggttggtt cacgtatgga ttgctattgg aaatgtcggc tactaaataa agattatcat
     3601 ctgcagaaat agctggtgct tcatctggat ttcgaggtaa atcaaccgca ttggtcacat
     3661 taatttttac aggtgttgtt ttataagttt ggcctccaat ttcaatcgat gcttgtttta
     3721 atgttaatgt tcctttttgc aaaggcatta aaatataaat atatgatttg ttaaacgaac
     3781 tttttccgtt tatccacgat tgactgatag attgacttgg accgccaaca actttgaaac
     3841 cacaagcttc aaaatttggt ggtacaaaat tgtcgccgtc agcattcata gtataatcaa
     3901 cgcgcaattt ttcgtttaaa cctaacgaat ttttgctcac tttggcttca aattctactt
     3961 gagcacaaag caaattgctg attaatatta ccgttaaaaa aagtattctt ttcatcttca
     4021 cttgtaaatt aaatagctat ttttcaatta ctaccaatct ttttcaggtt ttttaggatt
     4081 aactcgcact ttttgttggt taactttatc ctgaattttt ttctcttcat tatttaccgc
     4141 atccaacata ttttgaattc tatcttttga aatacctcca ggttgcggtt ttggctgacc
     4201 gtttttgtcg ttatttttgt cttgttggtc tttgttttta tcgccttctt ttttctggtc
     4261 gtttttgtct ttgtctttat cttttttgtc cttgttatcc tttttatctt gatctttttt
     4321 gtcttctttc ttgtccttgt ttttgtcttt attcttgttt ttatcgtctt cgcttttagg
     4381 cggattgttt tccaattgtt ttttggctaa tgcaaaatta tatcgtgttt cttcatcgtt
     4441 gggattgttt cgtaatgcat ttttataggc ttctactgcg ccttcataat ttttttcggt
     4501 catcaagcaa tttcccaaat tgtgataggc tttgtgtttc tcgggctttg tttttgctga
     4561 ttttatggct tttacaaact gaaatttagc ttcggaagct tgtttttgtc ggtaaataga
     4621 atttcctaaa ttataagaag caactgcttt tttaggaaat ccagattgcg aaacacgata
     4681 atcagcttcg gcatttgaat aatttttttc cgaaaaagct tcgtttccat cgggcaaatt
     4741 acggtctttt tccttttttt gggcaaacgt caaaaaggaa agcaagagta aagtgtatgt
     4801 tagtattctt ttcatctatt ctttttcatt aaacaaattc atttttcgaa cccatttggt
     4861 tttcttttcc aacaagaaca tatctaaaaa cagcagaaaa aatccaaatc ccaaaaacca
     4921 ttgaaattgc gatttaaagt ctgccatttg agtgctttca aactcggttt tttcaatgtt
     4981 ctccaaagca ttttttacat aagttaaaac atctttggta gaatttccat ctacataacc
     5041 gcctttggtg cttttggcaa tgaggctcaa tacttctgca tttcttttgg ttataacaac
     5101 ttcattattt tggtcgcgct gaaaactttc aacaacaccg tttcttttca atggaatagt
     5161 gccgcctttt tcggttccaa caccaaccgt gatgattttt aaacctactt ttttggcttc
     5221 ttcggcggca gttacggcgt tttcggaatg gtcttcaccg tcggaaatga taatcaataa
     5281 cttattggtt ttatcttttt tatctacaaa agttgttgct aaattaatcg cttcatcaat
     5341 cgatgttcct tgtgtggata caattcctgg attcatgctt tgcaaaaaca tttttgctac
     5401 actataatcc gttgtaatcg gcaaaacagg aaaagcactt ccggaatagg ctataattcc
     5461 aatgcggtcg ctacctaatt tgttgatgat ttgagaaacc aactgcttac ttttttccaa
     5521 acggtttggc gcaacatctt ccgccaacat acttttagaa acgtcgatag caaatataat
     5581 atcaattcct tcccgtttta cggtttctaa tttggttccc atttttggat taatcaatcc
     5641 aataattaaa caaactaatc ctagtataac gactataaat ttaaaaatag gtttgaaggt
     5701 cgatttttct ggacttaatt tttcaatcaa attcaaatcg ccaaattcgc gttgtttttt
     5761 acgtttccaa tacaataagt acaagaaaac caacgtcaat actggaatga ctagtaataa
     5821 ataaaaatat cctttttctt ctaactcgta catgtttttt tttcttaacc actaagttca
     5881 caaagttttt acactaagtt cactaagatt tgtttactaa agtacactgt gaatgttgag
     5941 cactcttggt attcattgtg tttaaatttt tatataaatc ctctaaaagt cgtatttcgt
     6001 aatccaaatt cgataagaat caaaattcct gctatccaaa cgaaaggtcg gtatttttca
     6061 tcataatcat agaatttcaa ttcttggatt tcggttgttt ctagtttatt aatttcttta
     6121 tatatttctt ctaatttact attactagtc gctctaaaat attttcctcc tgtattttta
     6181 gcaatcgttt gaagcaaatt cacgtcaatt tctactggca tcattttaaa taaaaatcct
     6241 tttccgtttg gggcatacgc ataaggaaac tcggccattc cgtttgttcc aattccgata
     6301 gtataaactt ttatgccaaa atcttttgca atttttgagg cggtttcagg ttcaataaac
     6361 cctgagttat tcacaccgtc ggtcataaga atgattactt tgcttttggc tttgctgtct
     6421 ttcagtctat taattgctgt tgtcaaaccc attccaattc cagttccatc ttgtaaaact
     6481 tgactgtatt ctatgttccg aatggcgtct tcaacaatgg ctttatcact agtaacgggt
     6541 gttttggtgt aagcttcggc agcataaagc acaataccaa ttctgtcgtt tggtcttcct
     6601 tccacgaaat tagacgctac ttttttcagg gcttccattc ggttgggttt taaatctttc
     6661 gctagcatac ttcccgaaac gtcgattgcc atcacaatat caaccccttt agttgatttg
     6721 gtttgactgc ttatgtcaac ggttcttggt cttgccatgg caatgattaa cgaacttaat
     6781 gctagaattc gaaatacaac caaatatggc tttaatttgg ttttccaagt agttgaaaat
     6841 ccagaaagtg aactcatttt tagtgttgct gtgctgtctt ttcgtttcca aaaaagccac
     6901 accatcgcta ctggaagcag taaaaataac cagaagaatt caggatttaa aaatgtaaca
     6961 tctttcatca tggttggcta taattttgta actctacaga atttaacact ctattcatta
     7021 tttctttgcc gtatttatcg tcttcgcgat gcattaccgt aatttgttgt agcccattgt
     7081 tttgactgaa tactaatatt tcataataca ttttggttga ttttcctttg attttatcca
     7141 tcatcgtcat tgttccataa gcttttttac cttttattcc tttttgggta tcaaaatctt
     7201 ctgttttgac caaaatattt tgtccgcctt gtgcttcaaa aaccttgatg cttccttctg
     7261 cagcttgcgt taaatcgatt tgtgtttctt gcttgaaact tgtcgttgaa actgcaatat
     7321 aaaaatcatc aatggcactc ccgtattgga aaatctgcat ttcttttagt agtgccattg
     7381 tttgttttgg caatactttt tcggcatcta ttcttttcaa tacttttgga gtttcgatac
     7441 gcaccgaagg gtttccataa tcactgtaaa tccattcgcc ttcaactaaa tctttggttg
     7501 gatgaccaat gatattgtct tttaaataat caaaaccttt gatatatact aatgttccga
     7561 gcgataaaac aataattcca agtacaattc cgatagtagt aatgatgcga acacgctttt
     7621 gttttttaag cttttcaatg cgtgcttttt ccttttgttg ttcattccac aaagccaatt
     7681 catcatcttc ttccacaatt tcaggaatgg atttatgaat agttacgata gtacttgaaa
     7741 tacgtttttt gtcttcttca atttcaaatt gtaccggaat ggatttagca aactttacta
     7801 aatcggcttg ttttaatacc ttttcaaggt tttcgacggt ttcgtttgaa agtttcaatt
     7861 tcttttgacg cgctgcattt cgcaaacctt caattaattc ggaagtcgtg ctttccattg
     7921 ctggaatttt aatttcttct tcaatataat ttcgagcaat atcagtaagt tctgaatagt
     7981 aatctttaat ttcgcctttt tgccaaagtt ctttggtttc caattgtaat aataatgtag
     8041 tcgctttttc aattggtgaa gtaaaaatca ctatttctgg ttttggttcg gcttgtttct
     8101 ttttgatgaa ataataaact aggtatccaa taccaccaat tgcgataaga attaacacat
     8161 aaatccacca attccctatt ggtttatcta cttgaatgat gttttttatg tcgtacattt
     8221 tttgttttaa tgtatcgact tttacatcgg caatttcgat ttggatactg tctgaaaaaa
     8281 cctttttacc attgattagc actggaatac taggaattaa atattttccg ctatcaaatt
     8341 gcgttaatcc atatttttta attaattcgt agcggcttcc ttttttaaca gtatcgattt
     8401 tataggattc taaaacttct aatcttccaa aagttttgtt ttttggaaaa gttactttag
     8461 cagttgtatc aacttctgtt ttgagagtta atttaaattc ggaaccaatt tttttctttg
     8521 tagaatcgat actcgttgtt actttttgag caaaaagcac attcgatagt aaaagaaaaa
     8581 ataaaattat tttaattttc attattaact tcttgattta aaataaccca ataatttagt
     8641 aacataactt tcgtcaacac gagtattgac cgttcctgaa ccgcatttag aaaaaatatc
     8701 tttaaagtaa tttacctttt cgtgataatt tttttcataa ttcattctcg ctttttttga
     8761 agttgtatca accgttatcc attttccact ttccgcatcc atcatttcta caacgccaat
     8821 attgagcatt ttttcttctc gagcatcata cactcgaatc ccagtaatat cgtgtttttt
     8881 tcctgcaatc tttaatgttt tttcataatc atcttctaca ataaaatcag aaatcaagaa
     8941 gacaattgct ttcttttttt gtgtagctga aagaaatttt aaggcttgcg ataattcggt
     9001 ttttctgctt tttggttgaa attctatgag ttcgcgaatg attcttagaa catgagattt
     9061 tccctttttt ggcggaatgt acaattcaat ttgatctgaa aataatatta aaccaatttt
     9121 atcgttattt tgcgtagccg aaaatgccat ggttgcagca atttcggtta caatttcgct
     9181 tttcaattga ttttttgttc caaaactttc tgaacccgaa atatctacca tcaacatcat
     9241 ggttaattcg cgttcttctt caaaaacttt tacatgggct tcattatatc gagcggtaac
     9301 attccaatca atcgcacgaa tgtcgtctcc atattggtat tgacgcactt cagaaaaagt
     9361 cattccacga cctttaaacg aggtgtggta ttcgcccgaa aagatgtgat cactcaatct
     9421 tcgggttttg atttctattt ttcgaacttt tttaagtagg tcttttgttt ccatttttct
     9481 ttgtttcagg tttcaggttt tctttgtttc aggttcaaaa ggataacttc gaacacgaaa
     9541 ctttaaactt ggaacaaaaa attaaggtac ttcaatttcg ttaacaattt tgttgatgat
     9601 gtcaaccgaa gtgatgtttt cggcttcggc ttcgtaggta attccaattc tatgacgcaa
     9661 cacatcgtga acaacagcgc gaacatcttc gggaattaca taaccacgcc gtttgataaa
     9721 agcataacat tttgctgcat tggctaaatt gatacttcca cgaggtgatg aaccaaaact
     9781 gattaatggt tttaggcttt ctaatttgta tttttctgga taacgagtgg caaaaataat
     9841 atccaaaata tatttttcta ttttttcatc catataaact tcacgaacgg cttcttgtgc
     9901 gcgaaggatt tgatctacag aaacgacttg gtttaccttt tcgtaacttc ctttcaagtt
     9961 ttgacgaatt accagtcgtt cttcatccat ttttgggtaa tcgatgacag tttttagcat
    10021 aaaacggtca acttgtgctt ctggcaaagg ataagttcct tcttgctcga ttggattttg
    10081 agtagctaaa actaaaaaag gacgttctaa tttgaaagta gtatcgccaa ttgttacttg
    10141 cttttcttgc atagcttcta ataaagccga ttgtactttg gctggtgcac gattaatctc
    10201 atcagcaaga acaaaattgg caaaaatggg acctttttta atggaaaatt cattttgctt
    10261 aatattgtat atcatcgttc cgacaacatc cgcgggtaat aaatcgggcg taaattgaat
    10321 tctactaaaa cttccgtgaa cggcttgtga caaagtatta atggctaacg tttttgctaa
    10381 accaggaaca ccttcaagca gaatatgtcc ttgtcctaaa agaccaatta ataatcgttc
    10441 caccatatgc ttttgaccaa caattacttt gttcatttcc atgatcaata aatcgataaa
    10501 agcactttct ctttcaattt tttcattaat cgctctaatg tctaaagcgg cactattttc
    10561 ttccatattt ttttatttaa actgtcgggg ttagtgttaa aatttaacat ttcaaaagtc
    10621 ataattaatt caagtgaatg atgttaacaa tgcgttaaaa cttttattct aaagcaaata
    10681 ttaaggatgt tttatgaatt tcttttgcta attttaagac ctaaatcttt ttttaaagat
    10741 tgtgtgccaa aataataaaa tttctaaaaa atatacaata ccacttattt tttaatcata
    10801 aacaataaaa actatgcaaa caaaactctc acctattgtt tcgggaacga tgaattgggg
    10861 aatttgggac aaaaacttat ccaccaaaga aatggtgcat ttaatcaata tatgcgttga
    10921 aaataaaatt accacttttg accacgccga tatttatgga aattatacca cagaagccga
    10981 ttttggaaaa gcattaaagg aaagtaaaat ggatagaaac aaaattcaat tgatttcaaa
    11041 atgtggtatt cagcacactc ttggaagaaa taatgcgata aaacattatg attattccaa
    11101 ggaatacatc acttggtcag ttgaaaattc cctaaaaaat cttcaaaccg attatttgga
    11161 tgttttgtta ttgcatagac caagcccttt aatgattgct gatgaaattg ccgaagctgt
    11221 ggaacaattg aaagaaaagg gaaaaattat tgattttggt ttgtctaatt ttaccagttc
    11281 gcaaacagaa ttaattcgcc aaaaaacaga agtaaactac aaccaaattc aattttcggc
    11341 aacgcatcat gaagcgatgt tggacggtag tttggattat atgcaaacac ataacattcg
    11401 accgatgagt tggaatccat tgggaaccgt tttcagagaa gatattgagc aaacgcgcag
    11461 actaaaaaaa cttttggtac aattagtaga aaagtacggt gtaggctcgg atacactttt
    11521 gttagcgtgg attttaaaac atcctgcaaa aataattcca gtggcaggaa cagtaaatat
    11581 tgctagaatt caacaattaa tgaaagctgt ggaactcaat tttgatacat tagattggtt
    11641 tgcaatttgg acggaaagca tgggaaataa agtgccttaa atgagtgatt agtaaatagt
    11701 tgttttttac taatcacttt ttactcttca ctaatcatta aaaaatgata aacaaacgtc
    11761 ttctcataaa aaatttatta gctcacaatg atgagagtac tttttatgac aaaaaacgtc
    11821 agttgaattt acactcaaaa gaaggaaaag ctaaattctt gaaacacatt tgtgcgttgt
    11881 caaactcaaa tccatctaac aattcgtata tcgttgttgg tgttgaagat gaaaataatg
    11941 aaatttcagg tgatgatttt tttgatgata gtcgaattca gaatctagtg aatgcttatt
    12001 tagaaaatcc tccaaaaatc ctctatgaaa atgttccttt tccaaatctt ccaaaagata
    12061 gagtaatagg tttagttaca ataaaaccca atcataaaac atcttttttt aagaaaaaca
    12121 tttatactat tactgctaat tcagttttta ttagaattgg aagcaattcg atgccaacgg
    12181 aagataaaat tccctactca aaatcaaaca tagaaaccgt cattagcatc gaaaacaatt
    12241 ctcgaaacag tattgctcat actttggatg gtgtgatgga ttttatgaac attcgtcatg
    12301 gcgatatgac ttccaaatac aaggttttta aagagttgtt tgtcgtttgt tgggcaggaa
    12361 tacctaagag aattagagaa actaattatt tatcgagagt tgatattgaa ttgattaacg
    12421 aacaaattaa attattttat tctaccttag atgtggttac aatttcgtat gacgaaaata
    12481 catttacgat aaccgaacat gttcaattag gcttaaatga caaaacgagt tattatcctc
    12541 tggaaaaagt gactattcat ttttatgaca atgggtatta taaaatggaa accaagatgt
    12601 tatttgaacc accaaaatac aatctaaaaa tgttgcatca catttataat tccaatctaa
    12661 cactgctttc taaattgcaa aaaaaaattg aattaaccga gcgagaaaac aaagatttat
    12721 ttcatttttc gcacacgttg atgatttgtt atttgaatgg ttttggcgat gcgaaacaaa
    12781 aaattattga tgcgaaacct tttttaaaag cacactcaaa tcctttagta tatttgaatt
    12841 ttaaggatgc tttgcgcatt ttacgaaaaa tgaaatacaa cgaaaatgac tagaacatta
    12901 gtaataggtg atattcacgg cggattacgt gctttacatc aaatcatgga acgtgctaaa
    12961 gtaacagcaa acgataaatt gatttttctt ggggattatg tggatggttg gagtgagtcg
    13021 ccacaggtaa ttgaatattt gattgcttta ggacaaaaac aagattgtat tttcattaac
    13081 ggaaatcacg acgaattatt gttggaatgg ctttcggaaa gtaaagataa tttggattgg
    13141 tacaaacatg gcggcgaatc aacggtttta tcctattcgc tatttccttc tgaaaccaaa
    13201 gagaaacata ttgaattttt gcaatcgtta aaaagtcatt atatagatga taaaaacagg
    13261 ttgtttattc atgctggatt tacaaacctt aatggaatcg attatgaata tttcccaaaa
    13321 cttttcagtt gggatagaac gctttgggaa atggctttgt gcttagacga tagaattcca
    13381 attgaaagtg aattataccc aagccgattg aaaaaatacc atgaaattta tattggtcat
    13441 acaccaacaa ctcgaattaa tgaaacaaaa ccactgcaaa aagccaatgt ttggaatgtt
    13501 gacacgggtg ccgcttttaa aggaaaacta acaattttag acatagacac caaagaattt
    13561 tggcaaagtg aacctgtttt tgaactctat cctatagaaa aaggaagaaa ttgatataat
    13621 tggttatttt tgtcgaacat taaacacata taaatgaaaa atattttaac gctagtagct
    13681 tttttgagta ttagtttact acatgctcaa gcatttaaag gaaaaggtga tattaaaggt
    13741 caaataggtt taacacttca aaatggagga acgggtatcg gaatctcttc cgattttggt
    13801 attggagaaa acatgtcatt tggttttgta gcaggttata tgctaaatgc cgataaaatt
    13861 gcagaggtaa aaccacgttt tgaagaccga gctgatttta aagtacgttt taacgccaac
    13921 ataggaaatg ttttgaaact agacgataat atggatgtat atccaggttt gagtcttgga
    13981 ttgagaaact ttggcggtca tgtaggattt aggtactttt ttaccgatgg ctttggaatg
    14041 tttgcagaag gttcagcacc tttagctcga tatgacaccg atgtaaatgg ttttgaacat
    14101 tacaacaatc agtttgtttt tattattgga acttccttca aattagacta aatgagtatc
    14161 gttactgaaa aaagagtaaa agaaagaagt ggttctgttt gcgaaattag cggaagcgat
    14221 gaaaatttgg tattatatat ggttgaacca aaaaccgaag acattcccga aaattgtatt
    14281 ttaattacac aaaaattaaa agatcaaatt gaaaacccag aaacaacgaa tgctaatgat
    14341 tggcgtggat taagtgacag catgtggaat gaaaatctgc ctgtacaaat tgtttcttgg
    14401 cgaatgcatg ctcgactaaa aaatatggat atgttagaga tgatgtatct tgatgaagat
    14461 gcattagaat gggcaaaagc aaccggcgaa gcagacgatg atgaagggaa aattattcac
    14521 aaagattcta atggtaacat tctaaatgat ggcgattcgg ttgtcttgat aaaagacttg
    14581 gatgtaaaag gagctaattt tactgcaaaa cgcggtgcag cagttcataa tataaaatta
    14641 gtttgggaca atgccgaaca aattgaaggc agagttgaaa accaacatat tgttatttta
    14701 acgcaatatg ttaagaagac aaaataacta gttaaaaata aatagtaaaa aaaagtgatt
    14761 agccaatttt gactaatcac tttttacttt ttactaatca ctaaaagact ataaatcaaa
    14821 cttaattccc tgtgctaatg gcaattggtc ggaatagttt actgtgttag tttgtcttct
    14881 catataaatt ttccaagcat cagaacctga ttctcttcca ccaccagttt ctttttcacc
    14941 accaaaagcg ccaccaattt cagcaccaga agttccgatg tttacattag caattccaca
    15001 atcagatcct gcaaatgaca agaatttttc tgcttctttc atttcgttag tcataatggc
    15061 agaagataat ccttgcgcaa ctccattttg taattcaata gcgttttcaa cttcaccaga
    15121 atatttcatt aagtataaaa ctggagcaaa agtttcgtgt tgcacaattt caaaatgatt
    15181 ttcggcttca ataatagcag gttttacata acaaccgctt tcataacctt cgcccgaaag
    15241 aacgccacct tcaaccaata cttttccacc ttctgctttt gctttctcaa tggcggctaa
    15301 ataagtattt acagcatctt tatcaattaa tggtccaata tgattttttt gatctaatgg
    15361 atttccaata gtcaattgtt tataagcacc cacaatggca tctctaactt tatcataaac
    15421 tgattcgtgg atgattaacc ttcttgtaga agtacaacgt tggccacaag ttccaacagc
    15481 accaaaaaca gcaccaggaa ctaccacttt taaatcagca gttggcgtaa tgataatggc
    15541 attgtttcct cctaactcta ataaggattt tccaaatcgc tcagcaacct ttgcaccaac
    15601 aattcttccc atacgagttg aacctgttgc agaaattaaa ggaattcttg tgtcagtagt
    15661 catcatttca cccactttat aatcaccgtt gatgatacaa gaaatacctt ccggtaaatt
    15721 gttttctttt aaaattccag caataatatt ttgacatgca attgaacata aaggtgcttt
    15781 ttcagaagct ttccaaacac aaacgtcacc acaaatccat gctaaagcgg tattccaaga
    15841 ccaaacagcc acaggaaaat tgaaagcaga aatgattcca acaacaccta aggaatgcca
    15901 ttgttcacgc ataacgtgac caggacgttc agatggaatg gtttgcccgt ttaattgacg
    15961 tgataaaccc actgcaaaat cgcagatgtc aatcatttct tgaacttctc cataaccctc
    16021 ttgaagcgat ttacccattt cataagaaac taattttcct agtggttctt ttaattctct
    16081 taatttattt ccaaattgac gcacaatttc tcctctttgt ggagctggca ttgctctaaa
    16141 agttagaaaa gcacttgttg cagcttgcat tactttttcg taatcttctt ttgttgtggc
    16201 ttttactttt cctattaatt gtccatcaac aggtgaataa ctttctatga tttccccaga
    16261 agaaaaccat tcatttcctg ttgaggttcc ttcgtttatg gcttttacac ctaatatttc
    16321 taatgcttcg gtcatcccga attgttgtgc aattgttgac atttaatatg ttttaatatt
    16381 gatttttaaa tattttacaa aaatagtggt tttttttgac ttattctcta aatcttagat
    16441 gcctgaaaaa aagctgtttt attttttttc catagattat tactgatttt atttgtcttt
    16501 aaaattaaaa tcagtgtaaa tctgtccaaa tccgttttgt ctgtggcaat tttattttac
    16561 aaatcgaacg tctgcttcca taactgttcc gcagttggtg caagttcgaa gttcttctga
    16621 gttataaaaa tgttcaaaat gtttcaaaaa atcgatttcg atatcgttta attcaaaata
    16681 gacttcatag agtttatgat tacaattgtc gcaaaaccaa agtaaaccat caggaatatc
    16741 atgaccaact ctttttcgct ctataactaa tccaatagaa ttttcttttc taacaggtga
    16801 atgtggcact tttgcaggaa gcaaaaacat gtcgccagca gaaagattca tagctttctt
    16861 tttaccatta tcttgaacat aaaccgtaat ttcaccttcg agttgataaa ataattcttc
    16921 tgtttcatta taatgataat cttttctggc atttggacca gccactatca taacaatata
    16981 atcacccgaa tcaacataca cgtttttgtt accaactggt ggttgcaaaa gatgacggtt
    17041 ttcatcaatc cattgattga ggttgaatgg tttttgaatg gacatgtttt tttttacgaa
    17101 gatactaaaa ataaaaaaag gttagctttc actaaccttt cggatattta ttttatttct
    17161 tttaaaagat agatgtacac aggaaagtga tcgctaaatc caggttcaga actcgaattc
    17221 cgaagcggat atcctttata ttggccagtg gtttgaatca tgtaaggctt gttaaatata
    17281 ccagctttcc aataacagaa agtggaataa tcttttctaa caaacgtttc ggaaaccatt
    17341 atttggtcaa aaatatcgcc tgaatctctc caaaacaaag aggcgttacc tttataaaac
    17401 atttcttcaa aagggttata aatatcagaa ggttgtttaa gttcttcttt tttacgtttt
    17461 gcgccaattc cttcttttac acttttatta taagttccat cgttcaaatc acccatggta
    17521 attatttttg catttggatt tatagcaaat aaggaatcca ttatctttct attcaatctt
    17581 ccagcagctt ctctaaacgg agagcttttc ttctcaccac ccgaacgcga aggccagtga
    17641 tttacaatga tgtttatttc ttcgccatca agaaatcctg taactaaaag ttggtcacga
    17701 gtataaactc tattatccgt tttagatgca gttgttacat cgtctgttgc ttcctctttg
    17761 tcttttttgt cttttttgtc ttcttttcct tcttgattgg aataaataat caaaggaatg
    17821 tttttgtaac tagtgggttt aaaatgtttt ttctggtaaa gaagaccaac atcaattcct
    17881 cttttatcgg gcgaatcaaa atggacaata ccataatcac gattgataag taaaggttct
    17941 ttcactaaat cttcaagaac acctctgttt tcaatttcac tacatcctaa taatgttgga
    18001 gaattacttg gattttcagt agagccaatt tcatttatta ccctggctaa attgtgtaat
    18061 ttttgtttgt attttttccc tgtccaattc atggcaccat taggcaacca ttcttcatca
    18121 ttatttggac cattaatggt atcaaataaa ttttcaaaat tataaaaagc tacagtatgg
    18181 atattatatt tttttccttg cccatttgag ctaaaaattg cgaataaaac aaagaaaacg
    18241 gctattaatt ttttaattct cataaattgt atattatttt aatatcaagt tttatacaaa
    18301 ctttgcgcca aaagtaaata atattattaa aagatgtaca tttgttgccc gttatgttaa
    18361 aaataaatat aacgaaggtg aatttttaac caatttttaa ttatgaaaaa acttgttttt
    18421 agtacattgt ttgtattgca aacttttttg atttttgctc aagcttcatc tggtgttaca
    18481 ggtaaagtta tcgattctaa atcacaaaaa cctttacaaa atgttgtagc ttcaattcaa
    18541 aacacaaatt taacagcact aacaaatgct ttgggagtat ttactattaa agatattagc
    18601 gatggttcgc aattgttaag aatatctagt gttggtttca aagaccaact tcttgccgtt
    18661 gaaattacaa aaggcgaaat aattgattta ggaattgttg ttttagaaga agacattaca
    18721 accgaacaac aattaagttt agttacatta accgagaatg atttaggcga tgacaacagt
    18781 ggttctgaaa gtacatcagg attattgcaa gcttcaagag attcttacca acaagcagct
    18841 gcctttaact gggggcaagc tcgttttaga ataagaggtt tagacaacga atatggaact
    18901 accatgataa atggggttgt catgaataaa attcttgatg gaagaccaca atggagtaat
    18961 tggggtggtt taaatgatgc tactagaaat caagagttta ccatgggttc tgccccatct
    19021 gattatacct ttggaggaat tcttggaaca caagaaatta atactcgtgc ttctacctat
    19081 agaaaaggaa cgagggtaac tttttcaggt acaaatacaa attattcttg gagaagtatg
    19141 ggtacatatg cttcaggaat gaatacggat ggatgggcat ttgtagtatc tggctcaaga
    19201 agatgggcac aagaaggcta ctttgaagga acagattata gtgcaaactc tctttttgct
    19261 agtgttgaaa agaaatttaa tgataagcac agtttaaact ttacttcaat ttatgcacaa
    19321 aatagtagag gtaaaagctc tcctaatact aaagaagtta atgatttaaa aggtataaaa
    19381 tacaactcct attggggatg gcaagacgga agaaaacgta attctagaga taaagacatt
    19441 gaagaaccaa tcaacatgtt aagtcattat tggaaaatat cagacaaaac aagtttaaac
    19501 acaaacgtgg cctatcaatt aggctacatt ggcaattcaa gaattgataa tcaaaattct
    19561 aaaaacccag atccatcgta ttatagagat ttaccaagtt attacttaaa taaccctgat
    19621 tcttatgtaa ctataggtgg tgtaactacc aatcctgaag cagatgccgc aagacaaaag
    19681 tttattaatg atggtcaact tgattggatt gcaatgtatg cagccaataa aagaagtgcc
    19741 aacgcaggaa aaagtgtcta tgtattgtac caagacagaa ccgatgacag acaattttcg
    19801 gcaaactcta ttttattctc taatctttca gataacataa gtttaaatgc tggcggtact
    19861 tatagaaaat taaaatcgca caattatcaa aaattagaag atcttttagg aggtcaattt
    19921 ttcttagatg tagatccatt ttattctgga gatcaaacac aatcagattt aaacaatcca
    19981 aacagaaagg ttgttgtagg agatgagtat ggatacaact ataacttaaa tgcagatgta
    20041 tttgatgcat tcactcaatt taagtttaat tataaaaaat ttgatttcta tttagcgcaa
    20101 tcattttcta aaagtgaata tcaaagagaa ggtttgtata gaaatggaat ttatgcaaat
    20161 agctctttag gatacagtaa taaggtaagt tttgaaaact ttggcttcaa aggcggatta
    20221 acctataaaa ttacgggaca acactatttg aattttaacg gagcttatat gaccaaagca
    20281 ccaggaatgc gtaatgtatt ctcaaatgct agaattaata acaacataac gcccgattta
    20341 caaagcgaaa atgttttcag tgttgatgga tcttatatca tcagaacacc aaaattaaaa
    20401 atgagattaa ccgctttcta tactaaaatt tctaaagcaa ccgaactttc attcttctat
    20461 ggtgaaggtt tatttgatga agctactgtt ggtgtaggaa acgaagattc tttcattagt
    20521 gaaattatta caggtattga taaaaagaat atcggcgcag aatttggttt tgaataccaa
    20581 ttaacctcaa ctattaaagc aacggttaat gcaagttatg gtcaatatac ttatgataat
    20641 aatccaaatg tctatttaaa tgatgatgct caagcatcat taacaaatac tagtccaatt
    20701 acaaactttg gcagtacata cctaaaagat tatagaatgc caggcatgcc acaacaagcc
    20761 tattcattcg ggttagaata tagagaccca aaattttggt ggataagtgc taatatcaac
    20821 catttagatg ataattactt agatgtatca gcagtaagaa gaacaacaaa ttttgttgat
    20881 gataacggaa tcggatatga tggagcaacg atagataacg taagaaaagt attgaaacaa
    20941 gaaaaatttg atgccttcca actattaaac cttgctggtg gaaaatcatg gagaattagc
    21001 tctaaaaaca gaaacactgt tggattcttt gcttctatta acaatgtcct agatgtgaca
    21061 tataaaacag gcggttttga gcaatcaaga aaagcaacct atccagacta tgttgctgat
    21121 aattcaaatg gaacaccttc ttttggtcct aaatattttt acggatacgg tagaacttat
    21181 ttcctaaacg tttatattaa cctataaaat aacgcactat gaaaaatata tttaaagtaa
    21241 ctttattttt tgtcttttct ttgacacttt tgtcaagttg ttctaaagaa gatgactaca
    21301 gcattccgcc ttataaacct ttgtttttta atgaaggttt tgaagaaatt ccaagagcaa
    21361 acactggagc aaatcaattt ataactttag aaggatggag taatgtatca ttaaatggcg
    21421 gagcaagaaa atgggaagct agatactatt ctgctgatgg tcagtacgct caacttcagg
    21481 catttcagag caacgaaaca aacatggata cttggctaat tagtccagca ttgaattttg
    21541 atttaactga aaatgaaggt ctaattttct cttacaagca agcattttat aatggtcaac
    21601 cactgtctgt cttaatttct accaattatg acgggagcaa tactgctcaa gccataaaca
    21661 atgcaacttg gacagacatg aatgttgttc ttccagattt tttaacaaca ggttatcctt
    21721 ctagtttttc aaaatctgaa attattgatt tatccgaatt tgatggaact gtttacatcg
    21781 cttttcgtta caaaggaagt tcctctggaa tttcaactac attccaattg gatgatatta
    21841 aattatttga aaataaataa cagctatgaa aaatttatta aaacttttaa caattacact
    21901 ttttgcagct ttaactagtt gtgtaaacag tgatgattat ggtactcccg atttaagtgg
    21961 agaatgtagc aatttaactg ctaccaaaaa caccccagac atcttggcaa tggcaacttc
    22021 aaacattcaa cagtttggac ctgcatacga caatgacatc attgaagcaa ttgtaacttc
    22081 tagtgatgaa ggcggtaatt tttacaaatc aatttcctta atgtcgctag atggtacaaa
    22141 aggatttagt atgccacttg atgattacaa tctatatact aaatacgaac caggaagcaa
    22201 agtgttcatt aagttaaaag gtaattttat tcaatacaac aatactactt caacggttga
    22261 aataggttcc aattaccaag gtaaagtagg aagaatttct ggaatatttt ataaagatgt
    22321 agttaaaaga agttgcgaag ataatgtagg tgaagataat attgttaacc acataactat
    22381 tgctcaagca aacaataata gttatatcaa caagctagtc gaattcgata atgttcaatt
    22441 tactgatgct tctattgggc atacctattt tgataaagac ttaaacccag tttctacctt
    22501 tactgctaca aaccatttaa ttcgtgacgc agatggaaat aaaataattg ttagagtgag
    22561 ccaatatgct aattttagcg gaaatcaagt tccatctggt aatggaaaaa tacgtggtgt
    22621 attaacaaaa tataatggtg attaccagtt tatgattcgt acagaagctg atgttaaatt
    22681 aacaaaccca agagtgcttc cattctatct agaaactttt caaacaactt ttgatgattg
    22741 gacaaaatat aacgtaactg gagcacaagt ttggtctcta tcagatgttg gtaatccagg
    22801 taaatgtgca gatatgaatg gttttgcttc tggcgcacaa acaaatgaag attggttgat
    22861 ttcaccttct cttgatttaa caagcgtatc ttctgctatt ttatcatatc aaacggcaaa
    22921 aaactttact ggtaatgcga ttcaagtttt aatttccaca aattacgttg gctctgcagc
    22981 accaagcact gcaacttgga cacctttagg aggttcaatt gccactgctt caaactttac
    23041 ttggaaagat tcaggagatg tttcattggc ttcttactta aataactcca atgttagaat
    23101 tgcatttaaa tatacgtcaa atacatctgg cgcagctcaa tggagagttg ataatgttag
    23161 aatagactag taaaaactta ttgattatta aataaagcct cgttttttcg gggctttttt
    23221 attttacaaa cttaattcac tacttttact tttcttaaaa attattcaat aatgaaaaaa
    23281 acagcacttc ttattcttag ctttatggca ctacaagcaa cagcccaaca agtactaact
    23341 cccgaaatct tatggaaatt aaaccaagtt tcagttttag gaatcaccaa agacggaaaa
    23401 tccatcatct ataaagtaaa aactccaact gtcgaagaaa acaaattctc ttcaaaaacc
    23461 tacagtttgc ccattgctgg tggaaatcca accgaaatat ccgataccaa aaacctaata
    23521 gttgataaaa atgtatcacc agatggcaaa tatgttcttt cttcaaacga agtaaaaata
    23581 gacaaagtac taggtaaaga ttattatccc gaactcgaaa aatcagacgt tcaaatctat
    23641 gacgcattag actatcgcca ttgggatact tggaacgaag gcttgcacaa ccatgttttc
    23701 tttggcgaaa ataatgcaaa tacaaaacca acagacatca tggcaaaaga accgttcgat
    23761 tgcccacaaa aaccatttgg aggagacgaa gactacattt ggtcaccaac cagcaaaagc
    23821 atcgtctatg tttctaaaaa actaacagga acacaatacg ccatttcaac caataccgat
    23881 ttatacgaat acaacataga aaccaaaacc acaaaaaatc taaccgaagc aaacaaagga
    23941 tacgatacca acccagcctt ttcaccaaac ggacaactaa cctggcttca aatgaaaagg
    24001 gatggatatg aagccgacaa aaacgacatc atcgttcgca taaaagacaa ctatgaccta
    24061 aatctaacgg cggcttggga tggaagtgtc gatagtttcc aatggagtgc cgatggtaaa
    24121 aaaatttact ttaccgctgc tgtaaacgga acaaaacagc ttttcgaagt aaattatcct
    24181 ggacttacca aaattgcagt tagagtaacg caaattaccg atggcgattt tgatgttaca
    24241 ggattagtag gcttttcagg tgaaaatgca atcctaacaa gaaccgacat gaaccatgcg
    24301 gccgaaatct ttagctataa caccaaaaag aaaacctgga cacagttatc aaataccaat
    24361 caagaaacat acagcaaact atcattacca aaatacgaaa gaagatatgt aaccaccacc
    24421 gatggaaaga aaatgcttgt atgggtaatc cttccaccaa actttgataa aaccaaaaaa
    24481 tacccaacac tattatattg tcaaggcgga ccacaatcac cattaacaca attttattcg
    24541 ttccgttgga atttctcttt gatggcttca caaggatata ttgtggtagc accaaaccga
    24601 agaggaatgt atggtcacgg gcaagcttgg aacgaacaaa tttctggcga ttggggcgga
    24661 caagtaatga acgattacct atcagcgatt gacgatgttt ctaaagaaaa ctatgttgac
    24721 aaatcccgtt tgggatgtgt aggtgctagt tatggcggtt attctgtctt catgcttgcc
    24781 ggaatacaca acaaccgttt caaaacattc attgcccacg atggagtata taacctagta
    24841 agcatgtatg gaacaaccga agaagtcttt tttgacaact gggaacacgg tggtgcctat
    24901 tgggaacaaa acccaattgc caaaaaaaca tacacaacct tcaacccaat tacccatatt
    24961 gacaaatgga acacaccaat cctaatcatt caaggaggac ttgatttccg agtaccaata
    25021 ggacaaggac aagaagcctt tcaagctgca caattaaaag gaattaaaag ccgattactc
    25081 tattttccac aagagaacca ctgggtacta aaacctcaaa acgccatcgt ttggcaaaga
    25141 gaattttaca aatggttaaa agaaacatta taaactccaa tgagcctcat ccaaaagatg
    25201 aggctttttt attatttaaa taatttttaa aaaaacttcc cggttttgct attttacttt
    25261 cgttttttat ttctctactt ttcttttgga gctttttctt tagtaattat gcctgtcaac
    25321 atttcagttg gataattatt ctttttgatt atatatctct acttttcatt tggagcttta
    25381 tcttttgtaa ttaagcctgt cagcattcca gttggataat tattcttttt aattatttat
    25441 ctctgctttt cttttggagc tttttcttta gtaattaagc ctctcagcat tccagttgga
    25501 tagtaaatat tgcttttttt tacaagtttt aattttggta atcaatattt tgtatatttg
    25561 attagcactt tatagtactg gtactctgtt ttatagtata aataatagac aataatatag
    25621 tttgattcat catattaatt taaaccaaaa acaacatgtt atacttcaac ctagtcgcta
    25681 tcatgaaaac gcgccaaata gaaagaccct atacctattt ggtaaaacaa ggtatttctc
    25741 cacatactgc ccacaaactt ttacatagtc aaactagaac cgttcgttta gaccatatcg
    25801 aaaggctttg tgaaattctt cattgtgatc ccaacgattt gattcgattt aaaccaaaca
    25861 gtattcaaaa acagcaaacg aatcatcctt tacagaaatt aattcaaaaa gaaactgact
    25921 ttgactggca aaattcgcta aaaacaatgc ctttagaaaa actaaaagag ctatctaagt
    25981 tgataactga tgccgaaaat aaagaagagt aattactaga aacaataaac ctcactaatt
    26041 agtgaggttt atttatttat aattcatcca ttttaaacgt atccataaag gcggtagtat
    26101 aatttccttc tacatatgcc ggatcatcca ttaattgtct gtgaaatgga atagtagttt
    26161 ttattccttc aattacaaat tcgtctaagg cacgtttcat tttgttaatc gcttcaccac
    26221 gagtttgcgc agtagtaatt aacttggcta tcatcgaatc gtaatttggc ggaatagtat
    26281 aaccagcata aacatgtgta tctaatcgca ctccatgacc tccaggagcg tgtaaaaccg
    26341 ttatctttcc tggtgaaggt ctaaagtcat tataaggatc ttcagcgttt atacggcatt
    26401 caatggaatg taactgtggt aaataatttt ttccagaaat tggaacacca gcggctacta
    26461 atatctgctc acgaatcaaa tcataatcaa taacttgttc cgtaataggg tgctccacct
    26521 gaatacgagt attcatttcc atgaaataaa aatttctatg tttatctaca agaaactcta
    26581 cagttccagc accttcgtaa ctaatatatt cggcagcttt tacagccgct tcacccattt
    26641 tatgtctcaa ttcatcagtc ataaatggtg aaggagtttc ttcggttagt ttttgatgac
    26701 gacgttgcac ggaacaatct ctttccgaaa gatgacaagc ttttccgtat gaatcgccta
    26761 ctatttgtat ttcgatgtgt cttggttctt caatcagttt ctccatatac ataccatcgt
    26821 ttccaaaggc agccgcagat tcttgacgag cactttccca tgctttttca agatcttctt
    26881 ctttccaaac ggcacgcata ccttttccac caccaccagc ggtagccttc atcatcacgg
    26941 gatatccagt tgctttagct acttttttgg cttcttcaaa gtcttctaaa attccatccg
    27001 aacctggaac gcaaggtact ccagccgcct tcattgtagc ttttgcagta gccttatcac
    27061 ccattttatc aatcatatca ggagaagcac caataaattt gataccatgt tcttgacaaa
    27121 ttttagaaaa tttagcattt tcagataaga atccatatcc tggatgaata gcatcagcat
    27181 tagtaatttc tgctgcagca atgatattcg acatttttaa ataagaaaga ttacttggcg
    27241 gaggaccaat acaaacagct tcgtctgcaa actttacgtg caaactttca gcatctgctg
    27301 tagagtaaac cgcaaccgtt tttattccca tttctttgca ggttctaatt actcttaaag
    27361 caatttcacc tctattggca atcaatattt ttttaaacat cttatttttt ttaaaaattc
    27421 aaaaaacaaa ttccaaattc caactacaag aaaaaaactt ggaatttaga atttaaaata
    27481 ttggaattta aagttatgat ggatctacta agaataaggg ttggtcaaat tcaactggag
    27541 acatatcatc cacaagaatt ttgacaattt ttccagaaat ttctgcttca atttcattga
    27601 ataatttcat agcttcaacc acacaaagta catctccttt tccgatggaa cttccaactt
    27661 ctacaaacat tggtttgtct ggtgctggtt ttctataaaa tgttccgatc atgggtgatt
    27721 ttatagttac atattttgaa ttttcttcag caggtgctac tgcaactgga gttgctggaa
    27781 ctgcagctgg agctggagcc gcaattggag ccgtttgcat ggcttgttgc atcggtgctt
    27841 gcaaataggt cacttcagga gcatgtcctt caagtgtagt tcggatagtt atttttacgt
    27901 ctcctgtttc cagctttacc tctgcaactc ctgtattgga tacaaatttg attaaatttt
    27961 gaatttcttt taaatccata atagttgttt ttaagaatga ttagtttatt gtttgttgta
    28021 tgcccactta aggtagatag aaccccaagt aaatccgccg ccaaatgcag caaagattaa
    28081 attatcgcct tttttaaatt tagattcaaa atcgcttaat aataatggta aagttgccga
    28141 agtagtattg ccatattttt ggatgtttac caatactttt tcttcttcta atcccattct
    28201 attagcagta gcatcgatga ttcttttatt tgcttgatga gcaattaacc aattgacatt
    28261 gtctttggtt aaattatttc gctgcataat tttttcactt acatcagcca ttccggatac
    28321 agcgtatttg aaaaccgttt ttccgtcttg aaatacaaaa tgttgacggt tttcaacagt
    28381 ttctatcgat ggaggaagga tggaaccacc agcatctatt tttaaatatt ctcttcctat
    28441 gccatcactt cttaagagtt cgtcttgaag ccctaggcct tcataattag gctcaaataa
    28501 gacagcacca gcaccatctc caaaaatgat acatgttgct ctatcggtat aatcaatgat
    28561 tgaagacatt ttatcggcgc ctattaaaag tacttttttg tatctgccag attcgatata
    28621 agcagcagca gttgacatgc caaataagaa acttgaacag gcagcctgca aatcataagc
    28681 aaacgcattt acagcaccaa tttgagatgc aacataaaca cttgttgatg caacaggcat
    28741 gtcgggagtt gtagtgccta gaattactaa atctatttct tcgggattaa tattcgcttt
    28801 tgaaattaaa tcttgggctg ctttgatggc taaataggag gttccttttc ctttttcttt
    28861 taaaagacgt ctttctttaa tacccgttcg agaagtaatc cattcatcat tagtttcaac
    28921 caaagtttct aaaacttggt tagaaagaac aaattcagga acataagacc caactgcggt
    28981 aattgcggct gttattttat tcataaaatg ttattttctt ttcttgattc gaaaagttgt
    29041 ggaaattaca aaattttttt aatccttctt taatttgaag cggatattta tcaaagttaa
    29101 tcaaaacaaa aaactctcac aaagtgagag tctgtttttt taataaaaaa ctttatttag
    29161 gcttcagcta ctgatttgtc aataactact tgtcctctgt agtacatttt accttcatgc
    29221 cagtaagctc tgtggtataa atgcgcttcc ccagttaccg ggcaagttgc aatagttgca
    29281 gcagttgctt tatagtgagt tcttctttta tctcttctag ttttcgagat ttttctttta
    29341 ggatgtgcca ttttattctt ttatttatcc gttaacagtt tttttaattc gtcccatctt
    29401 gggtcaatat cgtcttcttt tttgtctttt tctttaattt cgttgacttg taaatctttt
    29461 agtttgtcta atgcttccga ttgcaatgtg ccatctttta cgcctggatg tattcttttt
    29521 aatggaattg ataacgcaat catttcataa atgtattgag ataaatcaat ttgatgttca
    29581 ccatgaggaa gaattaataa ttcatcgttg tcgttgttaa agacatcacc aaattgaact
    29641 attaatttta atttcccttt aattggcaaa tcaaacaatt ctcctgttaa atcgcaagga
    29701 acattaacag ttccgttgtg tttaaacatg atttcgagca tggttgcctt tttttcaagc
    29761 acaacagcag ctttaatttc acaactttca aattcatcaa aactaaattc gtcaaagaac
    29821 tttttgtcta tagtatattc aaactgatgt tttcctagct ttaaccctat aaaagggatt
    29881 aaatattcat tcgtactttt catctaaaca acaatttgcc ccaaactttc gggagtgcaa
    29941 agatataaaa ttattgcaat tacaataagc ttatcaacat ttttttgttt ataactgctt
    30001 ttcttttgtt ttcaagggat tagaagtgat ttcttcatat tcatttcttg aattataaat
    30061 atctatggca gcataaactg cttctttaaa cgaattatag tctgcaattc cttttcctgc
    30121 aatttcataa gcagtgccat ggtcgggtga agttctaatt ttgtttaaac ccgcagtata
    30181 atttacgcca tttccaaaag aaagtgtttt aaacggaatt aatccttgat cgtgataggt
    30241 tgcaataacg gcatcgtatt tttgatattg attacttcca aaaaaaccat cagccgcata
    30301 aggtccaaaa actaaagtct tcttttcaaa tatttttttg ataattggtt ttaataattc
    30361 gtcatcttct ttgccaataa caccattgtc tccagcatga ggattaagcg ctaatactgc
    30421 tattttaggt ttattaattc caaaatcttg catcaaggat ttcttaatgg tttcaatttt
    30481 ctgaaggatt aacttttcat tcaaatggtt ggccacttca tttacaggaa catgatctgt
    30541 aaacaatcca actctcaaat tatcttgaat cataaacatt aatgcattcc cttcaagttc
    30601 ttggtttaaa taatctgtat gaccaggaaa tttaaattca tcggattgaa tattgtattt
    30661 attgattggt gctgttacta atacatcaat tttattttct ttcagagctt tagttgcagc
    30721 aacaaaagat ttaatagcat attctccaac tttaacgtcg tttacaccaa agttcaaatc
    30781 aacaccttct cgccaaatgt ttaaaacatt tatttttcca attacaatct gatcaatttt
    30841 atcaattccg tgaagttggg cggttaaatt caaatgtttt ttaataaaag agaggatttt
    30901 tacattagca aaaatcacag gtgtacaaag ttctagcatt cttgtatctt caaatgtttt
    30961 aagaattact tcgcttccaa ttccgtttaa atcaccaatg gaaattccaa cgataatatt
    31021 ttctgctttt ttcataagaa agacttgtta tttattgcta attttgaagt gcaaatttag
    31081 taaaataaaa ctacaatgtt tacaggtata attgaaaccc ttggtgtaat taaagaaatt
    31141 agaaaagaag aggataattt acagataacc atttcttctt ccataaccaa tgaattaaaa
    31201 attgaccaga gtgttgcgca caatggggtt tgtcttacag taatagccgt cgagaacgat
    31261 tgctacacag ttaccgctat taaagaaact atttcaaaga caaatttgag tgattggcga
    31321 gttgaagata tcgttaattt agagagagcc atgaggcttg gcgatagatt agacggacat
    31381 attgttcaag gacatgtgga ccaagttggt ctctgcaaat caatagtaga aactaatgga
    31441 agttggtgtt ttacttttga atacgacaaa tacttacaaa acattaccat tgaaaaagga
    31501 tctataacaa taaacggagt aagtcttacc gttgtgaatt ctaaagaaaa tgaatttagt
    31561 gtagcaataa ttccatatac ttttgaaaac actaatttca aaaacttcaa agttgcttca
    31621 agagtcaatt tagaatttga tgtgatagga aaatatgtca gcaaacttta tagtagtaaa
    31681 aacgaaaaag cttcaaatta atttgaagct ttttttatgt tgtgatgttt atatacttta
    31741 taaattccaa aaactacaga aattaccaat aatacgtaca aacttccgtc aataggcaaa
    31801 cctggcggag gcggaggcgt agatggcgga ggcggatttg atgcgccaaa aacaacgcca
    31861 cacggcagtg ccatggctaa aattagtgct attagtctat ttgggacaat tctcataggg
    31921 cgtaaaaata taaaaaaaaa ctaatggtca aaataaaata gtaatataaa tgttaaaagt
    31981 taactataaa ttaaaaattc aaaggtatca aatttatggt gaaatagtga aaaccattaa
    32041 ctttagtaat atataacgtt caaaattaat atacgaaata tatataattt tagttttaaa
    32101 ataaaaaaga ctccaatttg aagtctaatt ttatttgtgg tcccacttgg gctcgaacca
    32161 aggaccacct gattatgagt caggtgctct aaccaactga gctataggac ctgctttcaa
    32221 aaaaagaaaa cacttatttt gaggttgcaa tattaccact attttttttt cgttgcaact
    32281 atttttttaa cctatttctt ggcaaagctc aattaaaacg ccatttgtac tttttggatg
    32341 caaaaaaaca acaagtttgt tgtcagctcc tttttttgga ctatcattta aaacaacgaa
    32401 accctcagcc tttaaacggt cgatttccga aaaaatattt tcaacatcaa aagctatgtg
    32461 atgaattcct tctccttttt tttctaaaaa tttagcaatc ggactttcga ggtttgtagc
    32521 ttccaaaagt tcaattttat ttggaccatt cataaaaaaa gatgttttta caccttcact
    32581 ttctacaatt tcttctttgt agggtggatt accaaataac ttttcaaaca agtggtttga
    32641 atcttcaatg ttttttacgg ctattccaat gtgttctatt tttctcatta aaaatgtttt
    32701 ctacaaaatt agaaaataaa aatctttaaa aattcaaaaa tccaaattcg ttaatcttca
    32761 atcaaaaatc ttgtcgtatt tttgcatcat ggaaacgaat agacaaaaaa aaattggtag
    32821 tgtacttcaa aaagatttag tagatattct tcaaggcgaa gtgcgtaaaa atggaatttc
    32881 aaatttggta atttcagttt caaaagttac cgtaactacc gatttgtcta ttgcaaaagt
    32941 ttatttaagt gtttttcctc aagataaagc caaagacttg ttggaagcag taaaaacaaa
    33001 tgcaccatta atcaagcatg atttgtcaca aagagtaaga ttacaattac gaaaagtgcc
    33061 gaatttggct ttctacatcg atgattcttt ggattatatt gaaaaaattg ataacgcact
    33121 ttcaggaaaa gaaaatccaa ttgacaatag agatttgtta gacaaacgca agaaatttta
    33181 atttgaattt ttcgctatac atagccaaaa gatacctttt tagcaaaagt aataataatg
    33241 caatcaatat tattacagga atagcttcat taggaatcat tgttggtaca ttagcattgt
    33301 ttgtagtaat gtcggttttc agtggattaa aagtatttag tttgtcattt tctaatgatt
    33361 ttgatcccga tttaaaagta tttccaacaa aaggaaaaac aataaacatc agcaattctc
    33421 aagaaaatca gttaaaaaaa gtaaaagata ttgctgttta ctccaaaata accgaagaac
    33481 gagtgttgtt ttattttaac ggaaaagaaa ttgtaactta tttaaaaggc gttgacgcaa
    33541 attttactaa aactaatgta gtttcaaaaa acctttttaa tggcgaatgg ctttcaccta
    33601 aaactcccga agtcgttgtt ggatatggta tttccgaaaa attatctttg ggtttatttg
    33661 attttaataa tccttttgaa gtatatgttc caagaaaagg aaaagcaatt atagaaagtg
    33721 aagccgaagc ttttaaccga tccaagctcg tgccaattgg aatttatgcc attagcgaag
    33781 atttagacag caaatatgtt ttttgcgatt tggaattggc tcaaaaacta ttagaatata
    33841 aacccaatga ggtttctgct attgaaatta aaatagattc acttgctgat gaaaatactg
    33901 taatcgaaaa actgaataca attttcaaaa ataaagttat cataaaaaat cgttcagaac
    33961 aaaattcaac gctttataaa atgctaaata ccgagaatat tgcggtatat ttaatattta
    34021 ctttggtaat tattattgcg cttttcaatt tggttggagc attgattatg atgattttag
    34081 acaaaaaagg aaacctaaaa accttattta atcttggtgc tgaaataaaa gaattgaaaa
    34141 aaatatttct gtttcaagga agtttattaa gtgtttttgg cggaattatt ggattagttt
    34201 taggaattat tattgtgcta attcaacaaa aatttaaagt agttatgatt acttctactc
    34261 tagcctatcc agttgttttt tcaattcaaa atgttttaat cgttttttca acgatagtgc
    34321 tacttggagt actttcaagt tggatagcaa gcagtagaat taatgctaag atgttggatt
    34381 aagaaaattg ataatccgca taaacttctt gaatttggtt taaagcctta aataattctt
    34441 cactagactc aagtgtaacc aattttaaac ggtgttcttt aaaattagaa attcctttaa
    34501 aataattcgt ataatgacga cgcatttcta caatgccaac acgttcacct ttccattcca
    34561 tcgaccaagt taaatgattt cttgcagctt caattcggtc ttgcattgtt ggttgagcta
    34621 aatgttctcc agtttcaaaa aaatgtttaa tttcgttaaa aatccaagga taaccaatgg
    34681 cggcacgacc aatcatcatg ccgtccaatc cgtatttatt tttatattct aatgcttttt
    34741 caggtgaatc gatatcacca tttccaaaaa taggcattgt aattcgagga ttgtttttta
    34801 ctctagcaat atgactccaa tcggaatgac ctttgtacat ttgagcacga gttcgagcat
    34861 gaactgttaa cgcttgcaca ccaatatctt gtaaacgttc ggcaacttca tcaatattta
    34921 tagaactttc atcccaacct aatctagttt ttaccgttac cggaagatta gttcctttaa
    34981 taactgcttt tgtcaatcgc accatcaaat caacatcttt caaaactcca gcaccagcac
    35041 ctttacaaac tactttttta acaggacaac caaagttgat atccactaaa tctggctgaa
    35101 cggcatcaac aattctagat gacatttcca ttgcttcttc atcaccgcca aaaatttgaa
    35161 taccaacagg tcgttcgtaa tcaaaaatat ccaatttcat tttacttttc attgcatctc
    35221 gaatcaatcc ttctgacgaa ataaattcgg aatacatcaa atcggcacca tgaagtttac
    35281 acaatcttct aaaaggtgga tcacttacgt cttccattgg tgcaagaagt agaggaaaat
    35341 caggtagtag tatgttgcca attttgggca tgaaatgata aattttttgc aaaaatacta
    35401 atttttgttg gtttctgctt tcaggtcttt taaatcaaaa ctttgcctta tatttgccga
    35461 ttaaatgcgt tagcattttt ttgtttaaaa tttgacagat attgaagatg aaaaacataa
    35521 ggaatttttg cattattgca catattgacc acggaaaaag tacgcttgcc gatagacttc
    35581 ttggagcaac gcaaacggtt actgctcgtg aagaaaaagc gcagttgcta gacaatatgg
    35641 acttagagcg tgaacgtgga attaccataa aaagtcacgc gatacaaatg gaatatgaat
    35701 ataaaggcga aaaatatatt ttgaacctta ttgatactcc tgggcacgtt gatttttctt
    35761 atgaggtttc tcgttctatt gctgcatgtg aaggtgcttt gttaattgtt gatgcagcac
    35821 aaagtattca ggcacagacg atttcaaatt tatatttagc tttagaaaat gatttagaaa
    35881 ttattcccgt tttaaacaaa gtggatttac caagtgcaaa tcctgaagaa gtaagtgatg
    35941 atattatcga tttattaggt tgtaaattag aagatattat tcatgcctcg gggaaaactg
    36001 gttttggtgt tgaaaatatt ttagccgcaa ttatagaaaa agttccagca ccaaaaggta
    36061 atgttgatga gccattacaa gctttgattt ttgattcaca ttacaatccg tttcgtggta
    36121 ttgaagtaat ttttagggtt aaaaatggtg aaataaaaaa aggtcagaaa attaaattca
    36181 tggcaacaga taatgaatat tttgctgacg aagttggtac tctaaagtta aatcaggttc
    36241 caaaaaatgt gatttcaact ggcgatgttg gttatttaat ttctggaatt aaagaagcta
    36301 aggaagtaaa agttggtgat actattactg atgcaaaaac gccaacaacc aatatgatta
    36361 caggatttga ggatgtaaaa ccaatggttt ttgccggaat atatcctgta gatacagaag
    36421 attacgaaga tttgcgttct tctatggaaa aattacaatt gaacgatgct tcactagttt
    36481 tcactccaga aagttctgcg gcattaggtt ttggtttccg atgtgggttt ttaggaatgt
    36541 tacatcttga aattattcaa gaacgattag aacgtgaata cgatatgaca gtaattacta
    36601 cagttcctaa cgtttcctat ttggcttata caaaaaaaga acccgaagtt ggatttgttg
    36661 taaataatcc gtctgattta cctgaaccat ctcgtttaga cagagtagaa gaaccattta
    36721 taaaagcaac tatcattacc aaatctgatt ttgtgggtaa tgttatgagt ttgtgtatcg
    36781 aaaaacgtgg cgtgattacc aatcaaacct atttaacaac cgaacgtgtt gaattgaatt
    36841 ttgacatgcc attagccgaa attgttttcg atttttatga tagattgaaa acagtttcta
    36901 aaggttatgc ttcgtttgat tattcaccaa ttggcatgcg aacttctaaa ttagtaaaac
    36961 ttgatgtttt attaaatgca caaaccgttg atgcactttc tgccttgatt cacgaagata
    37021 atgcatataa cattggtaaa aaaatgactg aaaaattgcg tgaattaata ccaagacaac
    37081 aatttgatat tcctattcaa gccgcaattg gtgcaaaaat cattgctcgc gaaacgataa
    37141 aagcattacg aaaagacgtt accgcaaaat gttatggagg agatatttct agaaagcgta
    37201 aattacttga aaaacagaaa aaaggtaaaa agagaatgcg tcaagtagga aatgttgaaa
    37261 ttcctcaaga agcatttatg gcggttttga aattgaatga ttaattattg tcatacaaac
    37321 attacagtgc accctctgta ctaaatgttt tgtaaaatag ctcaactata ttagaaataa
    37381 cttttaaagt tgaagctcaa aattaaaaat aggcaacctt tttaaacgag tttaatctat
    37441 cgggaaataa ctgtacttat catcaataat cgatttgttt aatcaagaaa taattccact
    37501 attaattaac gaacaaccag tctttaatca agtagtactg atgcttaaaa aagtagataa
    37561 gaaaacacca aacaatacca atctatcact tcattctgac caggtttggc actatcaaat
    37621 aagtcattat tagtatttat taaataatta aaaaatatag ataaatatat aaagacaaga
    37681 atccagtcag atttaaacaa aatgagcccg atacaatatc gagctcatta ttatcaaaat
    37741 tatttataaa atttgtctaa acttttgggt tcattccatt ttttccaatt gtaaaaattg
    37801 agaatatatt attccttaat aaaacgctta gtagccgttt gatttcttga agtaatttca
    37861 attaagtagg cacctgtttt taagtttcca accaaaacag cattatcttc gatgttacca
    37921 ctttctaatt cttgacccat taaattaaaa attctgtaag tggcatcacc ttcaatattg
    37981 gcaatgttaa gtaagtttcc ttttactggg tttggatata aagtaaaagt taaagctgtt
    38041 gaagaaatgt cttccattct accactagcc gatatgttta tggtataatc ttcaacttgt
    38101 ccataagaga acgattcaca agatgtagga attccattgt atttcattga aactctcatt
    38161 cgtgtacttc ccaataaagc cgaagcagga atggtaaatg atcctgaaac tggtgttgtt
    38221 ttagaagctg tttttgtcca aactgtttcg ccagcatcaa caaaatcgcc gtcttggtta
    38281 taatcaataa atacagcata gccttcatta tatttggtgg aagtccatga tggtgttatg
    38341 gtaattgtgt tagcacttcc tctaacgaca gttgtagata aagctgtaaa attttcataa
    38401 cctgttgtgc ctgttgacgt attatttata gcaccaaaaa ccactttacc tattttctca
    38461 tcagcagtgc tatttccttg cgaagtacaa taagaaacac tattagacaa tgtagtaaca
    38521 tttgctacat tactcatcgc agaattattt ccagcagcat caaatgatct aactgtaaac
    38581 gaataagctg ttgaagcagt caaacctgtg atattagaac tggttgtagt aaccgttcca
    38641 attgaacttc catttaaaaa cacctcataa cccgtaaccg ctacattatc cgaagaagca
    38701 ttccaagtca aagtagttgt agtttgtgta gttcccgaag caaccaaatt cgttggagtt
    38761 gacggagcag tagtgtcgcc agcagccaca acaatgttta ctgtataatc ttctacttgt
    38821 ccgtaagaga acgtttcaca agctgttggt gtaccattat atttcattga aactctcatt
    38881 cttgtagcac cagaagttgc accagttggc accgaaaatg ttccaacagc aggtgtagtc
    38941 gttgaagtag ctttactcca aacagtttca ccagcatcat caaaatcacc atcttggtta
    39001 taatcaataa atacggcata accttcacta taggtgcttc cagtccatgt tggcgtaata
    39061 gtaatagtag cagctgcacc ttgagttaaa ttggtggata acgaagtata atctacatat
    39121 cccgaaatgc caccagaagt attatcaatt gaacctattt gtactcttcc aataaattcg
    39181 tcattaatac tgtttccttg cgaagtgcaa taagtaattc cagcagaaag tgtagtaaca
    39241 ctaaccgtat tacttgcaac agaaacattt cctgccgcat cttttgctct tactgtaaat
    39301 gaataggctg tagaagcaga caatccagta actgcataag ttgtagaagc agtagaaccc
    39361 aacaatgccg ctccttgata aacatcatat cctgtaacac caacattatc tgtagcacct
    39421 gaccatgata agttagttgt cgtttgagta gttcccgaag ccgccaatgt tggagctgtt
    39481 ggagcttgtg tatctccagc aggtgccgaa gtagtaacac taactgtatt acttgccact
    39541 gaaatatttc ctgcagcatc tttggcttta accgtaaaag tataagtagt tgaagccatc
    39601 aatcctgtaa cagcatatgt tgaagtagca gtagaaccca acaatgccgc tccttgataa
    39661 acatcatatc ctgttacgcc aacgttatcc gtagcaccag accaagataa gttagttgta
    39721 gtttgagtag ttccagaagc tgccaatgtt ggagccgttg gagcttgtgt gtctggagca
    39781 gaaccagcag taatcgtaaa gttagtattc gaaacgtcaa agaaaatatg attcgttcct
    39841 tttaccataa ttctacaaga agtacttggt gtatttggaa tagttacagc ttgcgttcca
    39901 tcatttggag tagcagccaa aatagtagtc cagttgtttc caccatctgt tgacaacaaa
    39961 acatcaacat ttgcacagtt tacaccattt gcagtcgttc ctgcaacagc ccaagtaacc
    40021 gtttgtgtag ttccaccaac ataagataca gcagtatttg gcgatgaaat cgtaaatggt
    40081 ccagctgaag ttgtaaccgt aactttcata tcgtcactat tgtttgctgg tccaccagct
    40141 ctgttatctc taacggtcag tctaaaattt aacgttcgtg ctacagaagg taaatactca
    40201 acattcaatt cgcttcctgc agtataaagt gctcctgaca ataccgaaga catcgttgga
    40261 aaatatcttg ttggagatga agaggaatta taagacctga aatttacacc acttgttttt
    40321 gtagcacttg gtacagctga agttgtttga gaatccattt gctcccagtt atacgtaagc
    40381 gcatcaccat tagcatctgt tgccgttcct gtcagcataa acggagtact tttaggcact
    40441 gtataatcca aaccagcgtt agccgtaggc acggaatttc ctgtggcagt attaacagaa
    40501 catgtttttg ttttcaaatt attagtaacc tgttgaatac ttatcgcatg aaaataagca
    40561 tccgaatgtg gctgaacatc tttagtagta attccagcat aacccataat tgttgagcca
    40621 cttcctggtt ccatttgaac accagttcct tcattactat gggtaaacgt atgattggca
    40681 ccaaattgat gacccatttc atgcgctaca taatccacat caaaattatc accggctgga
    40741 ataccgtctg caggcgaagt aaatccactt cctttatttt tgtcggtagt actagaagta
    40801 tcatcaacac aaatacaacc tatacaaccc gcgtttccac caccaccaga tgcaccaaac
    40861 aaatgtccaa tgtcataagc agcatttcca ataacagaag tcaaagtgtt ttgtaattcc
    40921 gtgttccatg caccaccagc tcctgtagaa gccgctgaat aaggatcggt agaagcatta
    40981 gtatagataa cagcatcggt attagcaatc aacaccattc ttgcaccaaa atctttttca
    41041 aaaataccat taacacgcgt catcgtatta ttgatagcag ccaaagccaa agcttttgta
    41101 ccgccaaaat aagacgtgta ttctccagta acggacatcg ccaatctaaa agttctcata
    41161 gtagcatcat cagcatttgg gcgcaacatt gttgctgaag cctctgttgg tttaataaca
    41221 tcaataaccg aacactcaaa cggtgtcaaa gaggctgctt tatccgattt tctatatact
    41281 gtataagtag tcaaatctgt agtgattggc tctataaact ccgctgcttt atcagcactc
    41341 aaaaccatcc ctttaaaccc caatggcgaa acactaaaat aaactgttgc cgaaggatta
    41401 tcaactccaa ttccaatata cgatttgatt tcaggataac gttccgccaa acctggctcc
    41461 ataatggaat tctcataaat tttataacgc tctaaagaac ccgaagcagt tggaattaaa
    41521 agtatatttt ccgattgccc ttttgtcgtt cgttttggtg ctgtggttaa actagtcctt
    41581 aatccgttta aattcaattc gaaaacttgg ttttctggta attgtaatct ttcattaaga
    41641 gcaaccttat cgttcatttt gttggtagca ttccatagcg aacttttact ttgtgcaaaa
    41701 gtaaaacttg caaaagccat caaagccacc gaaagtaatt tgtacttcat aataattttg
    41761 gtgttagtta aaaatagatt ttaaatgtag aattattttt tatacatatt taaaccaaaa
    41821 ctctaattta ttaacaaatc gacaaactac atgtttatat tttaaagcat ccgcataaat
    41881 aaaggcgttt gactgaaata tgcataatta ttttttaact acttgtgaat tatttctgta
    41941 aaaagtgtat ttttatcaaa aaaatttcca aatgaatcta agttattggg aacaaaaaac
    42001 atggttttct gatgtagatt acaccatagt aggtagcgga atcgtcggat tgcattgtgc
    42061 cttgacgtta cgagaaaaat atccaaatag taaaatatta attctagaaa aaggaatgtt
    42121 gccacaaggt gccagtacaa aaaatgctgg gtttgcttgt tttggaagta tctctgaaat
    42181 catagacgat ttaaaatcgc atactgagga agaagtggtt caattgattc aaaaacggtg
    42241 gaatggtttg caattgctta gaaaaaacct tggagatgca gctatagatt tcaaaccttt
    42301 tggaggttat gaattgtttt taaaagatgc tgaaagcagt tataatgaat gtttacaaaa
    42361 aattccattc attaatgaag tactaaaacc attcttcaaa aatgatgtct tcaccaaaga
    42421 agtagatcgt tttaatttca aagaaatcaa agaatacctc atcttcaatc catttgaagc
    42481 acaaatcgac actggaaaga tgatgcaaac cttattacaa aaagccattt ccaacaatat
    42541 tctcatatta aatcaagtaa tggtcaacag ttattctgaa aacacgaatg gcgttgaagt
    42601 agcactaaac gatttcagct tcacaaccaa aaaactattg tttgccacca atggttttgc
    42661 acaaaagtta actaataacc acgtgaaacc cgccagagcg caagttttaa tcactgagcc
    42721 aattccaaat ttagacatca aaggaacatt tcatctcgac aaaggttatt attattttag
    42781 aaacatagac gatagaatat tattaggcgg cggaagaaat attgattttg aaggagaaac
    42841 cacaaccgaa ttagcccaaa cagaaaaaat tcaaaatcgt ttggaagatt tgctaaaaga
    42901 agtaatttta cccgatacca atttcaaaat cgcacatcgt tggagtggca tcatgggaat
    42961 tggcagcagc aaaaacccaa ttgtcgaaca tttatctgaa catgtatatt gcggcgttcg
    43021 actaggcgga atgggtgttg caatcggaag tttaatagga aaagaattag cagaattagt
    43081 acaagcatga tcaaaaaaat actaaacttt ataaagaaaa cccttttgtg gtttttcgct
    43141 atttccatac tttcggtcat tattttcaaa tgggttccag tacctattac tccattgatg
    43201 gtcattcgcg ttgtagaaag taaatttgaa gattccgaaa ctattttcag tcacgattgg
    43261 gagccgttag agaatatttc acccaatctt caaaaagcag taatagcgag cgaagatgga
    43321 aactttttaa aacacagcgg ttttgacttt gatgccatgc aaaaagcatt taaaaacaat
    43381 aaaaaaggaa aacgcattaa aggcggaagt actatttcgc aacaaacagc aaaaaacgta
    43441 ttcctttggc aaggcagaag ttatttgcga aaaggcttag aagcttattt cacagtgcta
    43501 atagaactca tttggggaaa agaacgcatc atggaagtgt atctgaatag catcgaaatg
    43561 ggtaacggtg tctatggtgc aaaggaagcc gccagacatt ggtatagcac aacagccaaa
    43621 gatttaacac cacaacaagc tgcaggaata gcagctatat tgcctaatcc aagaaagttt
    43681 aaagcgtcca actcttcttc ttacattaac agaagaaaag ccaaaatcca acgtgtcatg
    43741 agacaaattg gtaaaataga atattaaaaa atacgccatc ccacgataaa acgggttaag
    43801 aaccacgtgc tgaaaataat tacttatcgt tttttctaaa ataaaatagc tccgaaattc
    43861 ggagctattt ttataaaatt tatgttattt aaaaaccata attcacacca acaccaataa
    43921 cttgacgcgt ttgaaaacct gcaaaagcgt tatcgtcata aatagtttgg aatgcaaaat
    43981 tggttgataa gtatttgttg attttcataa caatattaag ggtataatca atatcaacat
    44041 tttgtgggtc ttcgatatag tttgaatata agtttaggat attttcaacc gaaacatttt
    44101 ccattaaatt aaatttgtag taaccgttta tagcggcacc aaactcaaaa cgggaagatt
    44161 taccttgttc aactccaaaa gaaggtccta aatcagtaaa atgagaatga actaaaatca
    44221 attttgatgt tgcaggcgca atatttactt tcaaattatc gctttttttc cacatcatac
    44281 ctggtccaaa ttgaaaatag gcaggtgaaa agaaatgtga tgttttacta ccagatgtta
    44341 aatcaaaacc tgaatcaaat tgcgtttgaa aattaaaaaa caatgagtaa aaccaatatc
    44401 ctccagcttt tttgcctgcc aaagaattaa aaactaaccg atcatccgat ttttgaagat
    44461 cttgatcttt cagctttgtc aaaccatatg cggcgatgat tttattgtcc caattccatt
    44521 gctcactttt ataattaaaa tcataattta tgccaatatt tcccgaaata ctactttgtc
    44581 ctccggctaa ccagttgtta aagctagctt gattggccaa aacggtaaac atcccttttt
    44641 ttgtccaacc cataggtttt tcttctttta gtttagcttc ggcttcttcg tttttcttta
    44701 aaagttcttt ctcattaact tgagcaaaaa caccatttac agcgaataaa aatacagctg
    44761 aaagtaaaat ttttttcatt ttattagatt taaatttttc gggtgcaaag gtacaaatac
    44821 atttatctaa tccaaaataa acacaactat ttgataaata atagattaat tatttctgtt
    44881 ttttaaacaa cggaacagcc gaacaagctt caccatacat aatgcttttg gcaacttttt
    44941 gaagatgttg cacagcaaaa atataagcgc tttcaggaac aggttttttt gaacaacctt
    45001 ttaaaattaa tggtttgtct tggtatttgg aataatcgag ctgatttaaa atttcttgat
    45061 acaatactgt atttaaatca tttatagtac ctaacactac tttttttgcg aatgatgctg
    45121 cataagttgt aactaaaata cttgcccaag ccggaataat agcatccgtt ttacaatgaa
    45181 ttgctacata atgattttga tattgtgacc aatcatgatt ttttaaagct tcacgaaaat
    45241 ctttttcttt caataaaaaa ccttcaaaaa gccattgtga aatatcaatt tgcgttcgaa
    45301 ttccaatagg ataatagtct tctaaatcga acacttcgag tacagaattg gcaactttat
    45361 taactatttc gtccattttt tggaatttgg aatttaatta tttggaattt aattatagca
    45421 ttcctaattc taattttgct tcttcactca ttaaatcttt actccatggt ggatcaaaag
    45481 taatttctac ttcacaggat ttaacagttt caattttttg gattttttct tccacttcac
    45541 gaggtaaagt ttcggctacg ggacaatttg gcgatgttaa tgtcattaaa actttcactt
    45601 cattgtcttc attaatcatc acgtcgtaaa tcaatcctaa ttcgtatata tcaacaggaa
    45661 tttctgggtc gtaaatactt ttcaatactt ttacaacatt ttctcctaaa ttgatatcgt
    45721 ctttaaattc ttccattttt tatttttttt aacatataag tcatttaagt ttttaatccc
    45781 ataattcgtt ataaagatta tttacgtatg ttttttgtcc ttcgtaaaat atatttttta
    45841 cattaaaatt tattaatatt ccaattggag cgtttaataa attcatgtaa gtcagtaatt
    45901 gagcttcaaa aactggatgt atttctgata cagattttaa ttctacaaca agtaagtctt
    45961 caatcaaaaa atctcatctc aatttagttt ctaaattaaa acctttataa ttaataggaa
    46021 tttcgagttc agatttaaaa ttaatgtttt ttaattcaaa ttcttttatt aaacattgat
    46081 gataaactgt ttctaataaa cctggaccaa tatttttatg aacttcgatt gcagcaccat
    46141 taactttata aactaaatct tttaaataac ttttcgttac attcattatt ttaaactaaa
    46201 acttaaatga cttatatggt ttattttaat attaattttt agcattatat gccaaagcat
    46261 acatttttaa ttgttttatc attgatacca aaccgttggc gcgtgttggt gacaagtgtt
    46321 ctttcagccc aatttcgtca ataaaatcag tatttgcttc taaaatagct tctggttttt
    46381 ggttggaaaa agctcgaatt agtattgcaa tgataccttt ggttaaaata gcatcactat
    46441 ctgctgtaaa aactattttt ccgttttcct cttcgccatg aacccaaact ttagattgac
    46501 aacctttaat gatattttct tcagtcttat atttttcttc aattaatggt agtgctttcc
    46561 ctaaatcgat gatgtactcg tagcgttgca tccagtcgtc aaacatcgaa aactcattta
    46621 caatctcgtt ttgtatttct tgtattgtca tttttttact tactcttcta atggttttgc
    46681 caacgaaaca attttatcaa ctaattgttg aatttcagct ggtggattat tatggtcaaa
    46741 cgcttttgtt tcgtagtttt tatcttgata acgcactttc aattctgcaa tggcagcgcc
    46801 atcataaaat cgtttttgag atggatcttt taaatttggc aattcttcaa gattaatttt
    46861 ctcaaaacaa cccactaact ctttccaatc agcgtctgaa atttttattt gttcagatgg
    46921 actatctgga attcttgcct gagaaattgt tgctatttga tttgaaatta ccactttcaa
    46981 atgataacca cgcgtatgcg catcatattg caaaactgct tttgaaatat catttttggc
    47041 gtcgttacta caacccttgc tcattaaaag cgttaataaa atcatggtaa ttgttttcat
    47101 attctttttg gtttaagaca acatcatttg tgcttttttt acggcttcta ccaaagcatc
    47161 tatttcttct ttagtattat aaaaagcaaa gctagctcta atggttccag gaattttata
    47221 aaaatccata atgggttgtg cacagtgatg tccggttcgt actgctattc ctaatttgtc
    47281 aataattgta ccaatatcat acggatgaat tccatctata gtaaacgaaa ttacggaagt
    47341 tttttctttt gcggtaccaa taatttttaa tccttcgatt tctaataatt ttttggttcc
    47401 atattccaat aaatcgtgtt cgtaatttgc aatgttatca aaacctattt ggtttaaata
    47461 atctattgcg gttccaagca caattccacc ggcaatattt ggtgttccag cttcaaattt
    47521 atgtggcaaa tcggcatagg tagtcttttc aaaagtaact gttgcaatca tttctccgcc
    47581 gccttgatat ggaggtaatt ttcgcaacca ttcttctttt ccataaagaa ttccaacgcc
    47641 agttggaccg caaattttat gtccagaaaa cacgtaaaaa tcacagtcta gtgcttgaac
    47701 atctggtttt aaatgcggca ctgcttgtgc gccatcaatt aaaattgctg cgccaacatc
    47761 atgcgctttt ttaatcataa attcaatagg attgatagtt cctaaagcat tggatatgtg
    47821 attaacggtt actattttgg ttttgtttga aagtaatttt tcatattctg acaggattaa
    47881 ttcgccattt tcattcatcg gaatgacttt caatgtagca ccggtttttt cacaaagcat
    47941 ttgccaagga actatattac tatgatgttc taaggcagaa actaaaacct catcatctga
    48001 atttaaaaga gaagcaaaac cattggcaac agcatttatt ccatgtgttg ttcctgaagt
    48061 aaaaatcact tcgtgagcaa attttgcatt gatatgtgct tggatttttc ctctagaaac
    48121 ttcatacgca tcggttgcta attgacttaa cgtatgaacg ccacgatgaa tattagcatt
    48181 aatttcttcg tagtattttg aaattgcctc aataacaact ttaggttttt gagaagttgc
    48241 tccgttatca aaataaacca aaggctttcc gtttactttt tgagaaagta ttggaaaatc
    48301 ggctcttatc ttttgtatat caatcatttt atttagaaaa attctaaata caaaagtagt
    48361 gaaaagtgca tggaatagaa aataaaaatc aatgcatctt ctaaaaattt ccaccagaac
    48421 cgccaccgct aaatccacca ccaccaaact ccattggaga ctcatttgtt gtagtaattt
    48481 cggaatgtac attggcattt atcaaaatag cttttacaag atcgaatgcc atcggattca
    48541 agcttttatc ttccttaaaa gtaattccta cggtattgtt ttgaactttt ttaatagaaa
    48601 aataaaccat tgcaaaaaga atgcttccgc ctaaaagcaa tgcgtattcg ataggcaaaa
    48661 tagaatagta ataacgaatg gtagcaaaac ctagtgccaa agacaataag cctatccaaa
    48721 aaaagaagcg gtctttgttt tttaaaccaa atgaaatata aaaaacaggt aatgcaaatg
    48781 tggcaaaata aaataatggc gcaaatggaa tttcttgatt tggctgtaat tgtaaatcaa
    48841 gcaaaactgt tgacaattct cgaactacat aataattcaa agaagcatat ccaagaacta
    48901 aactgaaaat tttaatgatt tggaaaacat tggtgtaaat aaagttagta cttcttttaa
    48961 cgagttgaag ttggaaaaaa tacaacccaa tggatagtaa aaatagtaaa attggtaaat
    49021 aataagaagg aataatatct tcttccgtta ctaaataacc aaccaatcct atcaatccca
    49081 ttaacgcaaa aaatg
//
LOCUS       DAOOBH010000007        44210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_1078_length_44210_cov_6.083682, whole genome shotgun sequence.
ACCESSION   DAOOBH010000007 DAOOBH010000000
VERSION     DAOOBH010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 44210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44210
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1078_length_44210_cov_6.083682"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="PK218_01830"
     CDS             <1..1683
                     /locus_tag="PK218_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HPW97280.1"
                     /translation="AEAPSDVVACDSYTLPQLSSEGNDYYTATGGPNGTGTIIAPGTV
                     ITSTQTIYVFKEAIIRTAFTCTDENSFTVTINHTPVIAAIADVNACNSNELPQLTTGN
                     YYTGANATGTMLPAGTSITSPQTLYVYADTNTTPNCATETSFYVNVFNVDELPDVTIC
                     ESYILPQLTIGKYYTGTNGTGTQLYAGNTISTSQTIYIYLESNFLPRCSDESSFVVTI
                     VDTPIANIVPLSLRTVCDEDGVNDGIYQYDLTNFNAAVLGTQSGTEFTVSYYESLANA
                     QSQTNAVTSSSLTTIFVRVNNTLTENCNDIKAINIIVNKLPEPTPKDGFVCIDTETGA
                     LLNSYTMYSGLSAAAHSFVWTNELGETVSTYTAFTATTPGIYTLTATNNATGCVSEPF
                     EVIVQQSEPAEITYVVEDDFSSNQRIIVTATGQGGDYEYQLDGGQFQDSNIFENVRSG
                     IHTITVRDKNGCGITNLEALVVNYPKFFTPNGDGYNDTWNISDLSGNQNAKIVIYDRY
                     GKIIKEIKPSGRGWDGTYNGREMLSDDYWFAVTYTDENQNSREFRAHFAMKR"
     gene            1812..4250
                     /locus_tag="PK218_01835"
     CDS             1812..4250
                     /locus_tag="PK218_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW97281.1"
                     /translation="MKLKLFLFLFISHFALIAQNSGSINGVVIDKALKQPMPYVNVSI
                     FDNDKLVTGGITKENGSFSLKDIPLKKLKVEIVFIGYKKIIRELNFSNENTSINLNTI
                     TLEEDAIQLKGVEIISERSTIEQKIDRKVINVGKDIIASGNTASDILNNVPTVSVDAQ
                     TKEISLRGNSNVRILIDGKPSNIDAAQLLQQIPSSSIKQVELITNPSAKYNPEGMSGI
                     INIILHKNSNEGFNGSINSGVTFAVTPKVNTALNLNYKVGKVNFYSNYGFNHGKNINF
                     GNINSERPGSQNQQDFDSNNKNTSHLLKFGVDFYMDDKNTFSLFTNQNISLSSGDGVN
                     SINYFDAALRDSKQFTRQNNENNTQTYDFAYKHDFTKKGETLDVQANYSNTDNPDESR
                     FDYIQQNPVTNTISLNDIQRDINYFQFNVDYVNPLTESVKLELGTETRLQNIKNNFDE
                     NVSEVANSYNRIGNSNFDFTRNIHSLYTNIGKQWEKWSAQVGVRIEQYEIDGDFKRVT
                     TSSTPTENINDQSKLTDKIFTAYPSVFLNYKMNDKNSFNFNYSRRVDRPSIGQISPIR
                     EWSNPLIESRGNPSLAPQFTNSFEVNYTHIAKIGSITGGAFYRLINAEIGRVIYKNPD
                     NPAQDIISYDNFDNNKAYGVEVSANLKFAKWWSVNASSDAYFKTVRGTVENGITGAQE
                     RGEVDVTVFNARLNNNFVATKNLRFNMFAMYRGADLGLQFERKPMYRMDVGASYNILK
                     GKGTITARFNDMFKTMKFAFDGSIPYRQVGEFNWESRSVYIGFNYIFGGGKNKALQRK
                     QRDNNETQSSGGML"
     gene            complement(4356..5363)
                     /locus_tag="PK218_01840"
     CDS             complement(4356..5363)
                     /locus_tag="PK218_01840"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPW97282.1"
                     /translation="MFHSKITGLGFYVPENIVTNDDLSKLMDTNDAWIQERTGIQERR
                     HVIRGEDTTTSMGIKAATIAIERSGVAKEDIDFVVFATLSPDYYFPGPGVSVQKELGL
                     KTVGALDVRNQCSGFVYALSVADQYIKTGMYKNVLVIGSEVHSTGLDMTDRGRGVSVI
                     FGDGAGAALLSREEDLTKGILSTHLHSEGQHAEELVLIAPGMGKRWVTDIINENDPND
                     ESYFPHMNGQFVFKNAVVRFSEVIMEGLEANNLQVSDIDMLVPHQANLRISQFIQHKF
                     KLTDDQVYNNIQKYGNTTAASIPIALTEAWEKGKIKEGDTVVLAAFGSGFTWASAIIK
                     W"
     gene            complement(5527..5859)
                     /locus_tag="PK218_01845"
     CDS             complement(5527..5859)
                     /locus_tag="PK218_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HPW97283.1"
                     /translation="MGKFVITTRKNGEYQFSLKAGNGQEILGSEGYTTKAACLNGVES
                     VKKNSQEDKRFDRLEAKNGKPYFNLKATNGQIIGTSEMYESVAARENGIASVAKNAPD
                     AAVDDQTA"
     gene            6128..6628
                     /locus_tag="PK218_01850"
     CDS             6128..6628
                     /locus_tag="PK218_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97284.1"
                     /translation="MTEKVKYNFFYIIQNFVFAIFAILFFIVVFYETVFNSNYIGFVL
                     MLILWFIAYLFTANSLRNTYRYISNLPAIEFTEFSLIDHQQNVIYHWNNIQKIELITR
                     KNTTFAVFKLKNIKEYIDQLNNTEKILFKIFPDVIPIQVNLTYIKGNNKNIFNSINSH
                     FSKYRS"
     gene            6857..7189
                     /locus_tag="PK218_01855"
     CDS             6857..7189
                     /locus_tag="PK218_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPW97285.1"
                     /translation="MGITKTEGFTDSQNELAVLTKALGHPARIAIIEYIIRVNSCICG
                     DIVNELPLAQPTVSQHLKELKNAGLIKGSVEGSSICYCIDENGFEKVKGFFKTIYNHI
                     EKKKTDCC"
     gene            7205..7672
                     /locus_tag="PK218_01860"
     CDS             7205..7672
                     /locus_tag="PK218_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HPW97286.1"
                     /translation="MKLSEIKSILPNLETVAFQLPNGTFVPEHFHVTEVGLITKNFID
                     CGGTVRKETVVNFQLWNANDFNHRLKPKKLLNIIELSEKVLGITDFEIEVEYQDTTIG
                     KYNLSFNGKNFELVNKQTACLAIDQCGIPAEKQKVRLSDMTNEPCCAPDGNCC"
     gene            7692..8324
                     /locus_tag="PK218_01865"
     CDS             7692..8324
                     /locus_tag="PK218_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase"
                     /protein_id="HPW97287.1"
                     /translation="MTASQSILFSEIENCIKQLNLASISTERKKTLQPLIAFIQAKVT
                     NKETIRLNLICTHNSRRSHLAQVWAQTAAAYYNLKNVFCYSGGTEATAMFPMLGKTLE
                     NQGFKIKMLSEGNNPVYAIKFSKNEPAIIAFSKTFEDDFNPESNFAAIMTCSQADEGC
                     PFILGAEKRIPITYDDPKVFDNTPQQAEKYEERSVHIATEMMYVFSQINQ"
     gene            8327..9397
                     /gene="arsB"
                     /locus_tag="PK218_01870"
     CDS             8327..9397
                     /gene="arsB"
                     /locus_tag="PK218_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPW97288.1"
                     /translation="MAVNHCTPAVERKKLGFLDRFLTLWIFLAMAVGVSVGYFVPSSS
                     GFINSFSSGTTNIPLAIGLILMMYPPLAKVKYEQMGKVFKNTKVLGASLFLNWIVGPI
                     LMFALALLFLKGYPEYMIGLILIGLARCIAMVVVWNDLADGNREYAAGLIALNSIFQV
                     LLYSVYAYVFITILPPYFGYTGFEVNISIGQIAESVGIYLGIPFALGIISRYTLIKLK
                     GEEWFQTKYVLFISPITLVALLFTILIMFSLKGELIVQIPMDVIRIAIPLVIYFVVMF
                     VVSFFVGKYFGADYSKSTSIAFTATGNNFELAIAVAIGVFGINSGQAFAGVIGPLVEV
                     PALIALVNVAFWFKKKYFKTEV"
     gene            9479..9946
                     /locus_tag="PK218_01875"
     CDS             9479..9946
                     /locus_tag="PK218_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 domain-containing protein"
                     /protein_id="HPW97289.1"
                     /translation="MTRSAFIANRLEEVLLNGTWIANTNYKAQIKNINWQQANQQVGT
                     LNTIALLTHHVNYYLGGLLNVFNGGALEIRDQFSFDMPPIQSETDWKNLCHDFLTNAE
                     LFIQHVSKIEEEQWDTVFVDERYGTYYRNIEGVIEHCYYHLGQISLIKKLVLK"
     gene            9994..10416
                     /locus_tag="PK218_01880"
     CDS             9994..10416
                     /locus_tag="PK218_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97290.1"
                     /translation="MKYKILFIIICFLFSFSVFSQKREEKVTLGYYCGYSGSGTSVVI
                     KFAQLLYDKKYKTLKELLYSKIPAENFLSVVICKRLAHKKIITLTELELKRIDELFHS
                     KEKVPICGGCTFFEEVELTKLLNSNEEVFSAMDYFFDS"
     gene            complement(10844..11764)
                     /locus_tag="PK218_01885"
     CDS             complement(10844..11764)
                     /locus_tag="PK218_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW97291.1"
                     /translation="MLQLQNISFSYLDQPTLKNVDFSLTKGKTLAIIGESGCGKSTLL
                     KLIYGLHDLNSGQIYWNDTEVLGPKYHLIPGMDYMKYLAQDFDLMPFITVAENVGKYL
                     SNIYKDKKNQRIAELLDIVEMSELAHVKAKYLSGGQMQRIALARVLALEPEVLLLDEP
                     FSHIDNFRKNSLRRKLFAYLKEKQITTIVATHDSSDVLSFADEALVMQNGTLLAQDTP
                     QNLYQNPKSHYIASLFGDVNTVTLDGKEILLYPHQLVVDKKGALQVVVLNSFFKGAVY
                     LIEATYNDHLLYFENDTALEKGSAIGLRIQ"
     gene            11895..13412
                     /locus_tag="PK218_01890"
     CDS             11895..13412
                     /locus_tag="PK218_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97292.1"
                     /translation="MGLKTFISLFTFITAFSFAQVGIGTTSPHSQLEIASSNQVTPAN
                     TDGVLIPKINTFPATNPTALQQGMLVYLTTTATFASTSRSPGFYYWDNTTTNWIGIQT
                     NPTSNWNLLGNAATSISTNFIGTTDNTDLVIKRNNIRAGYIGNPSTSPFTNANTSFGA
                     NSLLNPTITGVVGEGTRNSAFGVNAMPGLTTGGLNTGIGEYTLFSATTGSANTAVGSA
                     ALYSVSTGRFNVAVGRNALVTSNGSNNIGIGTSALNGNSSGNNNIAIGTSSFRLNSTG
                     SNNLALGNQAGYNETGSNKLYIEMSTAVSGTDSSENNALIYGEFDNRIVRTNGQLQIG
                     NPTTSGYTFPTTRGTNNQILQTNATGTLSWVNSINNFSLVRANLTANQVVNATGWQKI
                     TFNTTLFDSGSEFVTATNRFVANKNGYYEINAGFHTFNKNDTEQYAIAVYKNGVEYQE
                     TAAHHYGDKLISRTINCTISLNVNDYIEIYMFNGNAPTTVDGFTGKTYFEVKQIR"
     gene            complement(13480..14790)
                     /locus_tag="PK218_01895"
     CDS             complement(13480..14790)
                     /locus_tag="PK218_01895"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HPW97293.1"
                     /translation="MKTLDKIPTGKIERASQLLKTGIKIGGNYVAYYGEKIVNPELNR
                     DKLNENNAEDIYDGLKNLKGSALKVAQMLSMDKSIMPQAYVDKFSLSQFSVPPLSAPL
                     VKKTFKKYLGKYPEELYDTFTPDAVNAASIGQVHKATKDGKTLAVKIQYPGVADSISS
                     DLALVKPIATRMFNLRGKDSEKYFKEVEYKLLEETDYVLELKQSQDVSEWCKKIENLR
                     FPEYFPEFSSEKILTMEWIEGEHLSEFTAHNSNRELGDKLGQALWDFYMYQMHYLKQV
                     HADPHPGNFIIDKNEKLVAIDFGCIKHVPEEFYVPYFELADPKVINNPKLFTDKLYEL
                     EILRNDDSPKEIEYFTKMFHDLLSLFTLPFHGNYFDFSDEEFFGKIAQMGEEFSKDTQ
                     LRKMNGNRGSKHFLYINRTFFGLYNLLHDLKARVDTKTFEKYID"
     gene            complement(14797..15465)
                     /locus_tag="PK218_01900"
     CDS             complement(14797..15465)
                     /locus_tag="PK218_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HPW97294.1"
                     /translation="MATTKKAKVKTAIIDETLIITHFMNEVLENNEEPKNVYVFCKKH
                     SIEESDFYSFFGSFDSLKETIWEKFFENALTTIQNDKNYASFSDKDKLLTLYFTLFEI
                     LTLNRTYIIFYLKENRNRLKNLKDLKRFRIHFKDFVKEIIGIDANPVFGKLNKIKEPT
                     YAEGAWLQFLFLLKFWLDDTSRGFEKTDVLIEKSVTTVMSLWDTKQFENLFDLGKFLW
                     KEKI"
     gene            complement(15604..16296)
                     /locus_tag="PK218_01905"
     CDS             complement(15604..16296)
                     /locus_tag="PK218_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW97295.1"
                     /translation="MKKITLILVSAFLIGNVFTGCEAVKNTNKTQRGAAIGTAAGAII
                     GGVLGNNIGKGGNGALGAVLGGVVGGVAGGVIGNKMDKQAREIETALPGAEVERVGEG
                     IKLVLGENAVRFDTNKSTLTATAKANLDKLIPVFNQYPDTDIQIYGYTDSTGAAEYNL
                     KLSGERAASVKSYLVGKGLSSTRFVTTGLGIADPIASNETVDGRSKNRRVEFAITAND
                     KMVQDAQKEAGK"
     gene            complement(16302..16775)
                     /locus_tag="PK218_01910"
     CDS             complement(16302..16775)
                     /locus_tag="PK218_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HPW97296.1"
                     /translation="MRKLILLFIFSFAIISCKSKSVTNTKLDIKSEVAIKGNWNITSV
                     SYPGSDVIKVMSFDLADSKCFIGSTWKFVSNNNKGNFALNSPSCTSYSTPITWYINKE
                     GNFVMKILDEAKAKTVKSGYILRVANQTESSFQLIDRIDVAGKMTDVVYQFQKLN"
     gene            16952..18838
                     /gene="htpG"
                     /locus_tag="PK218_01915"
     CDS             16952..18838
                     /gene="htpG"
                     /locus_tag="PK218_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPW97297.1"
                     /translation="MSSGKINVSVENIFPLIKKFLYSDHEIFLRELISNATDATLKLK
                     HLTSIGEAKIEYGNPIIEVKIDKEGKKLHIIDQGVGMTAEEVEKYINQIAFSGAEEFL
                     EKYKDSAKDSGIIGHFGLGFYSAFMVASKVEIITKSYKDEPAAHWTCDGSPEFTLEPS
                     EKTTRGSEIILHIAEDSLEFLEEFKIRELLTKYNKFMPVPIKFGTRKEKLPLPEGAEK
                     DAKPEEIEVDNIINNPNPAWTKQPTELKDEDYKEFYRELYPMQFEEPLFNIHLNVDYP
                     FNLTGILYFPKMDANLQMQKDKIQLYQNQVYVTDNVEGIVPEFLGMLRGVIDSPDIPL
                     NVSRSYLQADGNVKKIANYITRKVADKLKSLFTENREDFEQKWNDIKIVLEYGMLSEP
                     KFYEKAGDFVLYPSVGDKYYTLAELKEAITPTQTDKNGKVVVLYASNKEAQHGYIEIA
                     KEKGYEVLLLDSPIVSHLIQKLEADNENLTFVRVDSDHIDKLIQKDEEQISKLSEDEA
                     SKLKTVLEELVPKATYTVQLEAMDSKAAPFIITQPEFMRRMKEMSQNGGGGMFGMGNF
                     PEMYNLVVNSNSELATTILNTDDKSAQESLVKQALDLAKISQGLLKGEELTAFVKRSF
                     ELIK"
     gene            18948..19337
                     /locus_tag="PK218_01920"
     CDS             18948..19337
                     /locus_tag="PK218_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HPW97298.1"
                     /translation="MTLEIQTPALLFSATSLILLAYTNRFLTIAQIVRGLKKTYDQEK
                     TKDIFLQIKNLNLRLSLIRYMQLFGVLCLFLSVFAMLVLFLNLQTAGIYLFGVSLFCL
                     LISLGLSFWEISISVTALRLHLQDLME"
     gene            19389..20150
                     /locus_tag="PK218_01925"
     CDS             19389..20150
                     /locus_tag="PK218_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPW97299.1"
                     /translation="MQTKTDLKLAVLIDADNVPYANVKEMFEEIAKYGTPTFKRIYAD
                     WTKPTVSGWKKVLLENAITPVQQYSYTSGKNSSDSALIIDAMDILYTGNVDGFCIVSS
                     DSDFTRLATRLREAGMKVIGIGEKKTLTPFITACDKFIYIEILKKEEADATNETDTKK
                     TSRKPKEKSIPLSKIDPKIIKLFADSIEDLEDENGWAFLGDLGNLMLKKKPDFDPRNY
                     GFPKLLPLIKSINKFETDIRETGNSNIKHIYVRIK"
     gene            complement(20189..23041)
                     /gene="carB"
                     /locus_tag="PK218_01930"
     CDS             complement(20189..23041)
                     /gene="carB"
                     /locus_tag="PK218_01930"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPW97300.1"
                     /translation="MPKDNSIKSVLIIGSGPIVIGQACEFDYAGSQSARSLREEGIEV
                     ILINSNPATIMTDPSMADHVYLLPLTTKSLIQILKEHPQIDAVLPTMGGQTALNLCLE
                     ADEKGIWNDFNVKMIGVDVNAINITEDREQFKQLLAKLGVGAAPAKIATSFLKGKEIA
                     QEFGFPLVIRPSFTLGGTGAAFVHTKEEFDEKLTYGLEMSPIHEVLIDKALLGWKEYE
                     LELLRDKNDNVVIICSIENMDPMGIHTGDSITVAPAMTLSDTTFQKMRDMAILMMRSI
                     GNFAGGCNVQFAVSPDEKEDIVAIEINPRVSRSSALASKATGYPIAKIATKLALGYNL
                     DELSNQITKSTSALFEPTLDYVIVKIPRWNFDKFEGSDRTLGLQMKSVGEVMGIGRSF
                     QEALHKATQSLEIKRNGLGADGKGYKNYEQIIEKLTFASWDRVFVIYDAIAMGIPLNR
                     IHEITRIDMWFLKQYEELFHLEKEISKYKIDTLPKELLLEAKQKGYGDRQIAHMMSCL
                     ESQVYKLREEMNINRVYKLVDTCAAEFKAMTPYYYSTFEAEIETADGKRYVDNESIVT
                     DRKKVVVLGSGPNRIGQGIEFDYSCVHGVLAARECGYETIMINCNPETVSTDFDTADK
                     LYFEPVFWEHIYDIIRHEKPEGVIVQLGGQTALKLAEKLSKYGIKILGTSFDALDLAE
                     DRGRFSELLEELNIPFPQFGVAENADQASALADVLDFPLLVRPSYVLGGQGMKIVINK
                     QELEEHVIDLLKNIPGNKLLLDHYLDGAIEAEADAICDGENVYIIGIMEHIEPCGIHS
                     GDSNATLPPFNLGEFVLQQIKDHTKKIALALQTVGLINIQFAIKDDTVYIIEANPRAS
                     RTVPFIAKAYGEPYVNYATKVMLGVNKVTDFTFNPQLKGFAIKQPVFSFSKFKNVNKA
                     LGPEMKSTGESILFIDDLKDDQFYELYSRRKMYLSK"
     gene            complement(23207..23368)
                     /gene="rpmH"
                     /locus_tag="PK218_01935"
     CDS             complement(23207..23368)
                     /gene="rpmH"
                     /locus_tag="PK218_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPW97301.1"
                     /translation="MSKRTFQPSKRKRRNKHGFMDRMASANGRKVLARRRAKGRHKLT
                     VSCEPRHKK"
     gene            23522..24037
                     /locus_tag="PK218_01940"
     CDS             23522..24037
                     /locus_tag="PK218_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPW97302.1"
                     /translation="MFHKNIKLVLAILIFAAAIWQFTESNIGNGIFLILLTFIPIFLY
                     YKNEYILLAFLKLRKQDFPGSKIWLDRIKNPETALVRKQQGYYNYLNGLMLSQTNLHQ
                     SEKFFKKAVELGLSMDMDLAVAKLNLAGIAMSRRRKMEATALLNEAKKLDKQNMLKDQ
                     ITMMKDQMKKI"
     gene            complement(24142..25035)
                     /locus_tag="PK218_01945"
     CDS             complement(24142..25035)
                     /locus_tag="PK218_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPW97303.1"
                     /translation="MKKNHAILTTIGIILISGLFVFAISGANKEKAVREGTSLYFPTE
                     IDFAGEQTPLQIADVKERLDKELVINANLHSSTTLILKRANRAFPIIEPILQKYGVPD
                     DFKYLAVIESGLVNAVSSAGARGVWQFMPDTAKEKGMEVNDYVDERYHLEKSTEAACK
                     YLLAAKDKFGSWTLAAASYNGGMSGVNKKIEEQQVTNYYDLALTDETSRYVFRILALK
                     EIMKNSGKYGFTLFPNELYTNIPTKKIEVDSTINNLATFALEQGINYKILKIHNPWLR
                     DKKLLNPTKKKYEIEIPTEGY"
     gene            complement(25091..25750)
                     /locus_tag="PK218_01950"
     CDS             complement(25091..25750)
                     /locus_tag="PK218_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW97304.1"
                     /translation="MEKIHVYFMPGLAASPTIFERIQLPNDQFEIHLLEWFVPKEKQT
                     LGDYAKKMAEQVHHKNIILVGVSFGGILVQEMKQFLKLKKVIIISSVKSNKEFPRRMK
                     IAKTTKAYKLIPVALFENIENLAKYAFGDYIKQRVKLYEKFLSVRDKKYLTWAIESVI
                     LWERTEIDDEIIHIHGLEDEVFPVKYIKEFIPVKGGTHIMILNKYKWLNENLPKIMLE
                     N"
     gene            26025..26327
                     /locus_tag="PK218_01955"
     CDS             26025..26327
                     /locus_tag="PK218_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPW97305.1"
                     /translation="MEVQQMMEINDNTFARQFETTLDGNLITVEYSFQERKIFLTRIN
                     LPEDFENEDFVDELIKNILDIAEERKLKVVPIYPRITKFFRKNKSYKELLPPGIRI"
     gene            26409..26750
                     /locus_tag="PK218_01960"
     CDS             26409..26750
                     /locus_tag="PK218_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97306.1"
                     /translation="MDRERLIALILLFFEDITKYYGSTTEITEGIVTDIDNIDANLTT
                     WNLSEFILIRSAYRNTGNKFMLEGKGLYYEMAAEKIIGLKELGRNNFEFIEQYSENVY
                     RITKLKFHYKY"
     gene            complement(27006..28334)
                     /gene="mtaB"
                     /locus_tag="PK218_01965"
     CDS             complement(27006..28334)
                     /gene="mtaB"
                     /locus_tag="PK218_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPW97307.1"
                     /translation="MENKKKVAFYTLGCKLNFSETSTIARNFQNEGFYRVDFEEIADI
                     YVINTCSVTENADKQFKQIVKKALKVNEKAFVAAVGCYAQLKPEDLAAVDGVDLVLGA
                     TEKFKITDYINDLSKNDMGEVHSCEIEEADFYVGSYSFGDRTRAFLKVQDGCDYKCTY
                     CTIPLARGISRSDTMKGVMQNAKEISAKGIKEIVLTGVNIGDYGKGEFGNKKHEHTFL
                     DLVIELDKVEGIERLRISSIEPNLLKNETIELVSKSRAFVPHFHIPLQSGSNDILKKM
                     KRRYMKELYVDRVNKIREVMPHACIGVDVIVGFPGETDEHFLETYRFLNDLDISYLHV
                     FTYSERENTEAAEMEGIVAKNVRAKRSKMLRGLSVKKRRSFYESQIGTNRTVLFESEN
                     KEGYIHGFTENYVKVKTPWNPELANTLHEIKLTHIDEDGSVRLEFLGVEV"
     gene            complement(28475..29650)
                     /locus_tag="PK218_01970"
     CDS             complement(28475..29650)
                     /locus_tag="PK218_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HPW97308.1"
                     /translation="MNYILFDGTVRNALLPFTFTRPVAEIRIGILTIREKWEKYLGYT
                     TTTLTEEYLSEKYPMVEMEDNIMINASFLPNEVLVEMVKNLEKNQAIFKEDEVIAFYT
                     NDAQEEVDFDTYDIQYYDDECISVQNTWDIFAKNDVAIREDFDLLTEGRISQIIPKSV
                     NVISSENIFIEEGAKMEFVTLNASTGPIYIGRNAEIMEGSVIRGPFALCEEAQVKLAT
                     KIYGATTIGPHCRVGGEVNNSVMFGYSNKGHDGFLGNAVLGEWCNIGADSNNSNLKNN
                     YEEVRLWSYETENFAKTGLQFCGLMMGDHSKCGINTMFNTGTVVGVSANIFGSGFPRN
                     FVPSFSWGGSSGFTTYLTKKAFEVAKVVMSRRHVEFTEQDVQILEHVFEQTKKWRKE"
     gene            complement(29786..30037)
                     /locus_tag="PK218_01975"
     CDS             complement(29786..30037)
                     /locus_tag="PK218_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPW97309.1"
                     /translation="MKKGIHPENYRLVAFKDMSNDDVFITKSTVETKETIEHEGVEYP
                     VFKMEISRTSHPFYTGKSKLIDTAGRIDKFKTKYAKHAK"
     gene            30266..30811
                     /locus_tag="PK218_01980"
     CDS             30266..30811
                     /locus_tag="PK218_01980"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HPW97310.1"
                     /translation="MENQTSPAKSALSYGLFFGLIMGIELILGYVLNIDPQTNSTYGI
                     AINVLNYLALPFIFIYIACNNYKNKLNNGFITFGQCLKIGVLVCVIAALVYGVFYTIF
                     QLFVPEYFNGMLDKMDKILRDKNAEMTQEQIDMAISMTKKFMSPAIAVPITIAIFAFV
                     GLLHSLLVGAIVKKDPINQSF"
     gene            30818..31768
                     /locus_tag="PK218_01985"
     CDS             30818..31768
                     /locus_tag="PK218_01985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW97311.1"
                     /translation="MNLSIVIPLLNEQESLPELHQWIVNVMTTHKYTYEILFIDDGSK
                     DDSWKIIENLSAENPNVKGIRFLRNYGKSQALHAGFARAKGDVIITMDADLQDSPEEI
                     PGLCEMILNQEFDLVSGWKKKRYDSVVSKNLPSKLFNWAARKTSDVQLNDFNCGLKAY
                     KNIVVKNIEVSGEMHRYIPVLAKNAGFGKIGEKVVQHQARKYGESKFGMSRFINGFLD
                     LITIWFLSKYGKRPMHLFGAFGVLMFLIGFISTGLIIILKLIKLYSGYPTILVTNNPL
                     FYIALTTMIIGTQLFLAGFLGEIILRTKSNEERYKISKEL"
     gene            31783..32286
                     /locus_tag="PK218_01990"
     CDS             31783..32286
                     /locus_tag="PK218_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPW97312.1"
                     /translation="MKNIELISQKIFEIRGLKVMLDFDLAELYESETRILKQAVRRNA
                     NRFPSDFMFELNASEIEILVSQNVIPSKSKLGGANPFAFTEQGVAMLSSVLNNDKAIE
                     INIAIMRSFVALRKYALTYEELTKRISEVEKQFPEIYKVLNYLMQNDNKDKNQKDRNL
                     IGYKKDK"
     gene            32286..34013
                     /locus_tag="PK218_01995"
     CDS             32286..34013
                     /locus_tag="PK218_01995"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPW97313.1"
                     /translation="MHIEQSILDKVNEWLTPIFDSKTQDEIKEMMTSSPKDLEESFYK
                     NLEFGTGGMRGIMGVGTNRINKYTLGKNTQGLSDYLKKSFPNENLKVVIAYDCRHNSD
                     TLAKVVADVFSANGIKVYLFSEMRPTPELSFALKYLNCHAGIVLTASHNPPEYNGYKV
                     YWQDGGQLVPPQDGEIIEVIEALNYNEIKFDANENLIEYIDNAVDEAFAKSTVENASF
                     NTSDNARRNLKICYTSLHGTSIKTVPSVLEKAGYTDVNIVPEQAIPDGNFPTVLSPNP
                     EEPEALTMALALADKIKADIVFGTDPDSDRLGVAVRNNEGKMILLNGNQTMVVMTHFL
                     LEQWKKSGKIDGKQFIGSTIVSTPMMLELASAYDVECKVGLTGFKWIAKFIKDFPELK
                     FIGGGEESFGFMVGDAVRDKDAVGAILLMCEIAAQAKEKGSSVFEYLQKMFVNFGYYK
                     EHLISLTKKGIEGADEIKQMMVELRENPLSEINGKRVVCVEDYQASTGKDFMNNETFK
                     LTVPKSNVLIYYLEDGSKICARPSGTEPKIKFYFSVNIQINSVEEIPAAETLLNSKIE
                     TIIAEMELN"
     gene            34013..35842
                     /locus_tag="PK218_02000"
     CDS             34013..35842
                     /locus_tag="PK218_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW97314.1"
                     /translation="MDENLKKIIPFALKFKSNIIWNVIYNVLYALFATLSFVALFPLM
                     EVLFKMNEAVTKLPVYTGIDKLGEYGKQYLQFYITKLSNENGQQYALLLTVALVISTF
                     LFKNLFNYLASQHLMKIKNGVLKDLRDKMFSKIIELPVSYYSEKRKGDVMARMLGDVN
                     EVKNSFFIILELIIKEPLTIIFAIIAMLKISLELTLFVFIFIPISGFVISKIGKSLKA
                     KSTKAQQENGYLISIVEETLSGLKVVKSYNSENYFKLLFQNSITRLLKLDNSIGKKNN
                     LASPMSEFMGIVVIAILLWYGGNLVLVDKTLNGALFIVYLGLAYNILTPAKSISKASY
                     QVKNGLAAAERVFEVLEVENLITDNPNAVKKTTFTDKIILENINFRYEDENVLKNFSL
                     EVPKGKTVALVGQSGSGKSTIANLLTRFYDVNEGKITIDGIDIKEIEMKSLRNLMGLV
                     TQDSILFNDTIKANIALGKPNATDDEIIDALKIANAYEFVKDLPEGINTNVGDAGNKL
                     SGGQKQRLSIARAVLKNPPIMILDEATSALDTESERLVQIALENMMQNRTSIVIAHRL
                     STIQKADVIVVMHKGQIVELGSHEELLTKKGTYSKLVSMQSFE"
     gene            complement(35844..36563)
                     /locus_tag="PK218_02005"
     CDS             complement(35844..36563)
                     /locus_tag="PK218_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97315.1"
                     /translation="MKNYIIYILLFSMGIFVSCKKEEEKPKVIYETTQKPTANIVVDT
                     TKVLVADLPIHMEGTNFLLFPISDLNFSKRMNYGSSNRNNSISYTISNYGEYEITGFL
                     NNIKFKEVGKDTLYSLTDKPVIIQSATYLKDHAVKNKQQLMVYVLEDMDTNKDAKLDE
                     SDIKALYISAIDGKNFTKLSTDFQELLDWKYLEATNSIFFRTVEDTNKNGEMDSNDKV
                     FYSKVNLLDKEWKVENYNPTE"
     gene            complement(36600..39077)
                     /locus_tag="PK218_02010"
     CDS             complement(36600..39077)
                     /locus_tag="PK218_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPW97316.1"
                     /translation="MKTKFILFSLFILLNSFSVFSQNATAKVAGIVLDENNIPVENVN
                     VSYQSKSSITDINGFYEITIPANQKVILVFTHLALKNITASMQLKPNEEFEFNVMMND
                     KSDQLNEIIITNDKRRVEGITTISPESIRLNPSANAGIESVLKSLPGVNSNNELSTQY
                     AVRGGNYDENLVYVNEIEVYRPFLIRSGQQEGLSFTNTEMAQNVDFSAGGFQAKYGDK
                     MSSVLDITYRNPKKFALAAEASFLGGSLTADFVSKNKKWSNITGVRYRDNSLLVNSQE
                     TQTNFRPTFVDVQSIVNFKPNEKWSFSFLGNASKNKYNYQPLTRQTNFGTIDEPIALQ
                     IFYEGQENDQYQTLFGAFKTVYEADKNNTFKFIGSVYHTQEQEYFDIFAAYFLGDVDT
                     NIGSENLGQVTFNRGIGTQLNHARNDLDALIFNAEIKGSHKLKNKKHIIDWGVKYTKE
                     NFRDRVVEWEVIDSTGFSINPPTFHIPNDQPYNPYVGPLVPYQNIRATNFVDINRISG
                     YTQWSFKDQIGSADYWVNFGVRIHNWQVTGKSITNGKNQTVFSPRAQFSLKPDWKKNM
                     LFKVAVGYYHQPPSYRELRDSLGMVRPNVKAQESIHFVLSNDYKFQMYNRPFKLVTEA
                     YYKSMTDVNTYTLENVRIRYRADNDAVAYAYGLDMRLNGEFVPGTESWLSFGYMKTEE
                     NLNDKGFIARPTDQRLKFGILFQDYMPNLPNMKLYLNLVYNTGLPGGSPSYADPYDYQ
                     LRLRDYRRADVGFSYVLTERNSEKPDGNWLKKFKDLSVGFEIFNLFNNQNAITNTWVR
                     DVYTKNQYGIPNYMTTRVFNIKLTARL"
     gene            complement(39087..40784)
                     /locus_tag="PK218_02015"
     CDS             complement(39087..40784)
                     /locus_tag="PK218_02015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW97317.1"
                     /translation="MKVFVFLLFVTSCTFGQNEYPRNYFVSPLEIPLQLSGNFGELRP
                     NHFHAGFDLKTNKKEGLFVYAAADGYISRIKITHGGYGKAIYITHPNGYTTVYGHLQS
                     GFEAIEAYIKREQYKAKSYEIEVFPMPNELIVKQKEIIGLSGNTGGSDGPHLHFEIRD
                     TASEKIINPMYFGFDTIIKDTKKPYLMGLYVYPLNDSSSVNQSKRRLLLNYDQTAEGD
                     IIVEKIKASGKIGFGIFVQDFDNVSWNTNGIFQVKTTLNGKLSFGYQFDTFSFDENKY
                     VNALIDYEVYKTQKIRIQKLFSKRYYPLSILKTDENLGVISVNSNENKTYKVEVSDFH
                     GNVLKINIPIVYSSEPITIKEISKNNKYFIKAKRETIFSLNNVTVTFPENTFYEDFPL
                     NFDVKNETVYLHQDIIALQNSFKLSIEDTISSEKDRQKMFIGYYDGKKYIHYTTKRYK
                     NTFSIYSKTLGQYKLIKDTILPSISIAKSIEGKWISNQKNIELTISDDLSGINTYEGY
                     LNGNWILFEYESKLKRLRHSFDDGIVTEGKNDLKVIVTDNVGNSTIFETQFYRSQKP"
     gene            40963..41253
                     /locus_tag="PK218_02020"
     CDS             40963..41253
                     /locus_tag="PK218_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97318.1"
                     /translation="MSEIAEIFGSVEIRVEKLLNKLNSLEANLLQKQSELNKAENIIK
                     NQENEIEALKKACESLKMANSLLGSEENKRDTKLKINSLIREIDYCITQLSD"
     gene            41258..41545
                     /locus_tag="PK218_02025"
     CDS             41258..41545
                     /locus_tag="PK218_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPW97319.1"
                     /translation="MGEKLKIKISIADRVYPLTVETSQEEGLRSASKKIDTMIKQFEE
                     NYAVRDKQDVLAMCALQFASQVEQKQLDKSIDGTETLEKLKKINDLLKDFA"
     gene            41607..41714
                     /gene="ssrS"
                     /locus_tag="PK218_02030"
     ncRNA           41607..41714
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK218_02030"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01685"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="flavobacterial type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01685"
     gene            41765..43330
                     /gene="rny"
                     /locus_tag="PK218_02035"
     CDS             41765..43330
                     /gene="rny"
                     /locus_tag="PK218_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPW97320.1"
                     /translation="MENIFIIILAPIIGIAIGFGIAKFLEKSNVSNLIKNAKKEATSI
                     LKEANQEAENIKKDKLLQAKEKFLELKSEHEKEILSKDKKISEVEKRVRDKESQVSSE
                     LAKAKKVNDDFENKTKEYESKIEILDKKHQELEKLHKSQVNQLEVISGLSAEEAREQL
                     VESLKAEAKTKAMAHIQDTIEEAKLTAQQEAKKVIINTIQRVGTEEAVENCVSVFNIE
                     SDDVKGRIIGREGRNIRALEAATGVEIIVDDTPEAIILSCFDPVRREIARLSLHKLVT
                     DGRIHPARIEEVVAKTTKQIDEEIAEVGKRTVIDLGIHGLHPELIKVVGRMKFRSSYG
                     QNLLQHSREVAKLCGIMAAELGLNVKLAKRAGLLHDIGKVPDTESDLPHALLGMQWAE
                     KYGEKEEVCNAIGAHHDEIEMKYLISPIIQVCDAISGARPGARRQVLDSYIQRLKDLE
                     DIAFGFNGVKNAYAIQAGRELRVIVESEKVSDDMASSLSFEISQKIQTEMTYPGQVKI
                     TVIRETRAVNIAK"
     gene            complement(43385..43780)
                     /locus_tag="PK218_02040"
     CDS             complement(43385..43780)
                     /locus_tag="PK218_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW97321.1"
                     /translation="MKFNDVFVVDDDKVFHFIIKKLLMNNGIDVNPAFFENGLLAIER
                     LKKKLENGESTPDLILLDINMPILDGWQFLEEFKRIKNKLEKDIIIYIVSSSDDKADK
                     SKAKTFEDEVKNYYLKPMTAEDLRTIFAV"
     gene            complement(43770..>44210)
                     /locus_tag="PK218_02045"
     CDS             complement(43770..>44210)
                     /locus_tag="PK218_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW97322.1"
                     /translation="LNMYLNKVLDILNNDISKNKVKIFNKIPKECYIDFNPSYLESIL
                     LNFTTNAIKYSHPERVPEIMYNHYLENGKVVLEIQDNGLGIDLARHGDKLFGMYKTFH
                     KNENARGIGLFITKNQIESMGGSVTVDSKTNKGTTFKIYFNNEI"
BASE COUNT        15106 a         7376 c         6942 g        14786 t
ORIGIN      
        1 gcagaagcac ctagtgatgt agtagcatgt gatagttata cattaccaca gctttcctca
       61 gaaggaaatg attattatac tgccactggc ggtccaaatg gaactggaac cattattgct
      121 cctggaactg ttattacttc tactcaaaca atctatgtgt ttaaagaagc cataattaga
      181 actgctttta cttgtacaga tgaaaactca tttacagtta ccataaatca cacaccagtt
      241 attgcagcta ttgctgatgt taatgcctgt aattcgaatg aactgccaca attaactact
      301 ggaaattatt atacaggagc caatgctaca ggtacaatgt tgcctgctgg aacttcaata
      361 acatctccac aaacattata tgtttatgca gatacgaata ccactccaaa ttgtgctacc
      421 gaaacaagtt tttatgtaaa tgtgtttaat gtggatgaat tgcctgatgt aactatttgt
      481 gaaagctata tcttaccaca attaactatc ggaaagtatt acacaggtac gaatggaact
      541 ggaacgcaat tatatgctgg aaatacaatt tcaacttctc aaaccattta catctattta
      601 gaatctaatt ttttaccaag atgtagtgac gaaagctcct ttgttgtaac aattgttgac
      661 acacctattg caaatatagt tcctttatca ttgagaactg tgtgtgatga agatggcgta
      721 aacgatggta tctatcaata tgatttaact aattttaatg cagctgtatt aggaactcaa
      781 tctggcactg aattcacagt tagctattat gaaagtttag ccaatgcaca atcacaaacc
      841 aatgcggtaa ctagttcgtc tcttacaaca atttttgtta gagttaataa tacacttact
      901 gaaaactgta atgatatcaa agcaataaac attatagtaa acaaactgcc tgaaccaact
      961 ccaaaagatg gatttgtatg tattgacaca gaaactggtg ctttattaaa ttcatatacc
     1021 atgtatagtg gtttgagtgc cgctgctcat tcgtttgttt ggacaaatga attaggagaa
     1081 acggttagta cttatacagc atttacagca acaacacctg gtatttatac acttacagca
     1141 acaaacaatg ctacgggttg tgtttctgaa ccatttgaag taattgttca acaatctgag
     1201 ccagctgaaa ttacatatgt tgttgaagat gacttttcta gcaaccaaag aataattgtt
     1261 acggctactg gtcaaggtgg cgattatgaa taccaattag atggtggcca atttcaagac
     1321 agcaatattt ttgaaaatgt tcgttcagga attcacacta taacagttag agataaaaat
     1381 ggttgtggca ttaccaatct agaagcatta gtagttaatt atccaaaatt ctttactcct
     1441 aatggtgatg gttataacga tacatggaat atttcagacc ttagtggaaa tcaaaatgct
     1501 aagattgtta tatacgaccg ttatggaaaa ataataaaag aaatcaaacc aagcggacgc
     1561 ggttgggatg gcacttataa tggtcgagaa atgctatctg acgattattg gtttgctgtt
     1621 acctataccg acgaaaatca aaacagccgt gaatttagag cccatttcgc catgaagcga
     1681 taacctacat aaaatatgtt taaaaaactc tcaatcattt ttgagagttt ttttttgttt
     1741 ttagtgtaac agaaccaaaa acaaatagtc taaagaaaca taaacaaaat tatttcaaaa
     1801 aatcatcaat catgaaatta aaattattct tatttttatt tatttctcat tttgcattaa
     1861 tcgctcaaaa ctcgggaagt ataaatggcg ttgttattga caaagcctta aaacaaccaa
     1921 tgccttatgt taatgtttcc atctttgata acgacaaatt agtaacaggt ggcatcacta
     1981 aagaaaatgg aagtttcagt ctaaaagaca tcccattaaa gaagctaaag gttgaaatcg
     2041 tttttattgg gtataaaaaa ataatccgtg aacttaattt ttctaacgaa aacacaagta
     2101 tcaatctaaa cactatcaca ttagaagaag atgctattca actaaaaggt gtagaaatca
     2161 tcagcgaacg ttctactatt gaacaaaaaa ttgatagaaa agtgattaat gttgggaaag
     2221 atattattgc ttctggaaac acagcttctg acattttaaa caatgtgcca accgttagtg
     2281 ttgatgcaca aactaaagaa attagtctta gaggaaactc aaatgttcgt attttaattg
     2341 acggaaaacc ttcaaatatt gatgcggctc aattgctaca acaaatccct tcttcttcca
     2401 tcaaacaggt tgaattgatt actaatcctt ctgctaagta caatcccgaa ggaatgagtg
     2461 gtattattaa tattattctt cacaaaaatt ctaacgaagg ttttaatggt agtatcaatt
     2521 cgggagttac ttttgctgtt acgcctaaag ttaacactgc cttaaactta aattacaaag
     2581 tgggcaaagt gaacttttat tccaactatg gttttaatca cggaaaaaac ataaactttg
     2641 gtaacattaa cagtgaaaga ccaggctcac aaaaccaaca agattttgat tccaacaaca
     2701 aaaacacttc gcatttatta aaatttggag ttgattttta tatggatgat aaaaatactt
     2761 tttcattatt taccaatcaa aacatttcac tttctagtgg cgatggtgtt aattcgataa
     2821 attattttga tgctgcttta agagattcta agcagtttac aagacaaaac aatgaaaaca
     2881 acacccaaac ctatgatttt gcatacaaac atgattttac caaaaaagga gaaacattag
     2941 atgtgcaagc caattattca aacactgaca atccggacga atctcgcttt gattatatac
     3001 aacaaaaccc ggtaactaat actatttcat tgaacgatat tcaaagagat attaattatt
     3061 tccaatttaa tgttgactat gtaaatcctt taacagaatc ggtaaaacta gagttgggta
     3121 ccgaaactcg tcttcaaaac attaaaaaca actttgatga aaacgtatct gaagttgcca
     3181 attcttataa tcgtataggt aattctaact tcgattttac tcgaaacatt cattcgcttt
     3241 atactaacat tggaaaacaa tgggaaaaat ggagcgcaca agtaggtgtt agaatagaac
     3301 aatatgaaat tgacggcgat ttcaaaagag taactacttc ttctactcct actgaaaaca
     3361 ttaatgacca aagtaaatta accgacaaaa tattcacagc ttatccttcg gtttttttaa
     3421 attataaaat gaatgataaa aattcattta acttcaacta ctccagacgt gttgaccgtc
     3481 caagtattgg tcaaatcagt cctattagag aatggtcaaa tccgttgatt gaatcaagag
     3541 gaaatccatc tttggcacct caatttacca attcatttga agtaaactat acacacattg
     3601 ccaaaattgg ttcgattact ggtggtgctt tctaccgatt aattaatgcc gaaattggaa
     3661 gagttatcta taaaaacccc gataatcctg cgcaagatat tatctcttat gacaactttg
     3721 acaacaacaa agcctatggt gtagaagttt cagctaattt gaaatttgca aaatggtggt
     3781 cagtaaacgc tagttcggat gcctatttta aaaccgttcg tggtactgtt gaaaacggga
     3841 ttacaggtgc acaagaacgt ggcgaagttg atgtaacggt attcaatgct agattaaaca
     3901 acaattttgt agctacaaaa aaccttcgtt ttaatatgtt tgccatgtat cgtggtgctg
     3961 atttaggttt gcaatttgaa agaaaaccaa tgtatcgcat ggatgttggt gcttcttata
     4021 acatcttgaa aggaaaagga accatcacag cacgttttaa tgatatgttt aaaaccatga
     4081 agtttgcctt tgacggaagc attccttacc gacaagttgg agaattcaac tgggaaagta
     4141 gaagtgttta catcggattc aactatatct ttggtggcgg aaaaaacaaa gcacttcaac
     4201 gcaaacaacg cgacaacaac gaaacacaaa gcagtggcgg aatgttataa tataacttga
     4261 aaggttaact aaaaaaccat ttagagcatc aagtaattta agaaataaat cttgataaac
     4321 atggtgctct aaatggttta atttctttta gaatattacc actttataat cgcacttgcc
     4381 caagtaaacc ctgaaccaaa tgctgccaat acaaccgtgt cgccttcttt gatttttcct
     4441 ttttcccaag cttcggttaa agcaattgga atggatgctg ccgtagtatt accatatttt
     4501 tgaatgttat tatacacttg gtcgtccgtc aatttaaact tgtgctgaat aaactgcgaa
     4561 atcctcaaat tggcttgatg tggcaccaac atatcaatat ctgaaacttg aaggttattg
     4621 gcttccaagc cttccataat tacttcgcta aaacgaacaa cggcattttt aaacacaaac
     4681 tgtccattca tgtggggaaa ataactttca tcgtttgggt cattctcatt aataatatcg
     4741 gttacccaac gttttcccat tcctggtgct attaacacta attcttcggc atgttgtcct
     4801 tccgaatgca agtgtgtaga gagtattcct ttggtcaaat cttcttctct tgatagcaac
     4861 gctgctccag ctccatcgcc aaaaataacc gaaacacctc tacctctatc ggtcatgtct
     4921 aatcctgtag aatgcacttc ggaaccaata actaatacgt ttttatacat cccagttttg
     4981 atgtattggt cggcaacaga aagcgcatag acaaagcccg aacattgatt tcgaacatcc
     5041 aaagcaccaa cggtttttaa acctaattct ttttgaacgg aaactcccgg accaggaaaa
     5101 taataatcgg gacttagggt agcaaagacc acaaagtcaa tatcttcttt agcaacaccc
     5161 gaacgctcaa tagcaatggt agcggctttt atacccatag acgtagtagt atcttcgcct
     5221 ctaataacat ggcgtcgctc ttgaataccg gttcgttctt gaatccaagc gtcgttagta
     5281 tccattagtt ttgacaagtc gtcgttggta acaatatttt caggaacata aaaacccagt
     5341 cctgttattt ttgaatgaaa catagtttga ttatttagta aaataagaag ctaaaagtaa
     5401 acatctttta aaaattgaca acacaaggta gttattattt cggaattagc aatcattgga
     5461 gaatgtattg cacaaaaaaa aaccgattca aaattaaatc ggtttttgtt ttattgctta
     5521 taaagattaa gctgtttgat catcaacagc tgcgtctgga gcatttttgg ctactgatgc
     5581 aattccgttt tctcttgctg ctacgctttc atacatttcg ctcgttccaa taatttgtcc
     5641 gttggtagct tttaggttaa aatacggctt tccgttttta gcttctagtc tgtcaaaacg
     5701 tttatcctct tgcgagttct tttttaccga ttctacacca tttaggcaag ctgctttggt
     5761 agtatatcct tcgctaccca atatttcttg accgtttcca gcctttaagc taaattgata
     5821 ttcgccattt tttctagttg tgattacaaa tttccccata attataattg attaaatgtt
     5881 taaaaacata aagctactaa aaattgttga gagtcacaat taaaagttcg ttaagagttt
     5941 aagggtttga tatggaaaac tgtaaaacag tatgaaaaag ctttttcact tttggtgaga
     6001 acaaaatcag ttggatgatt tgtaatgagt tttttatata tatttgataa acgctataat
     6061 gtatgacttt atcatatata taaacaagtt ataaataact tcaaaactaa tcggacagaa
     6121 aaaaatcatg actgaaaaag taaaatataa cttcttttac attatacaga attttgtttt
     6181 tgcaatcttt gcaattctat tttttattgt ggtgttttat gaaactgtat ttaattcaaa
     6241 ttatattgga tttgtcttaa tgctaatatt atggtttata gcatatctat ttacagctaa
     6301 cagcttacga aatacttatc gttatatatc caatcttcct gcaattgaat ttactgaatt
     6361 ttcacttatt gatcatcagc aaaatgtcat atatcattgg aataatattc aaaaaataga
     6421 actaattaca cgaaaaaata ccacgtttgc agtatttaaa cttaaaaata taaaagaata
     6481 tattgatcag ttgaataata ctgaaaagat tttatttaag atttttccag atgttatacc
     6541 aattcaagtt aatctaactt atataaaagg taataataaa aatatattca attcaataaa
     6601 tagtcatttt tctaaataca gaagctaacc cgccattttg catttgcaat ctatactttc
     6661 tatactaaac cgctcaactt ccccactaca aaaaatcagc atttcaatcg ctgcttaaaa
     6721 aaatccgtcc aaatccgtga tgcaatgcat ccgttttatc cgcgttcaaa aaaattaaca
     6781 caaaacaaaa ttaaaatcac tgttttcttt tttgttttaa aaacatatcg taatattgca
     6841 atataaatat atattcatgg gaataaccaa aacagaaggc tttacagata gccaaaacga
     6901 acttgccgtt ttaaccaaag cactaggaca tcctgctcga attgctatta tagaatacat
     6961 cattagggta aattcctgta tctgcggcga cattgtaaac gaattgcctc tggctcaacc
     7021 cacggtttcg cagcatctaa aagaactcaa aaacgcagga ttgataaaag gaagtgtaga
     7081 aggaagctct atttgttatt gcattgatga aaatgggttt gaaaaagtga aaggcttttt
     7141 taaaactatt tacaatcata ttgagaaaaa gaaaaccgat tgttgttaat taaaaaatca
     7201 tattatgaaa ttatccgaaa ttaaaagcat cttgccaaat cttgaaacag ttgcgtttca
     7261 attgccaaac ggaacttttg ttcccgagca ttttcacgta accgaagtgg gtttgattac
     7321 caaaaacttt atcgattgtg gtggaacggt gcgcaaagaa acagtagtta acttccaact
     7381 ttggaacgcc aacgatttta accatcgact aaaacccaaa aaacttttaa acatcatcga
     7441 actgtctgaa aaagtattgg gcatcactga ttttgaaatt gaagtagaat accaagatac
     7501 tactattggt aaatataatt tgtcctttaa cggaaaaaac tttgaattgg taaacaaaca
     7561 aacggcttgt ttagccatag accaatgtgg aataccagca gaaaaacaaa aggtaagact
     7621 ttcagatatg accaatgaac catgttgcgc tccagatgga aactgttgct aaccaactat
     7681 tcaaagaaga aatgacggca tcgcaatcta tattgttttc agaaattgaa aactgcatca
     7741 agcagttgaa ccttgcaagc atcagcacgg aacgcaaaaa aacgttgcaa ccgctgatcg
     7801 cttttattca agccaaagtg accaacaaag aaaccattag gctgaatcta atttgcacac
     7861 acaattcgag aagaagccat ttggcacaag tttgggcgca aacagcggcg gcttattata
     7921 atctaaaaaa tgtcttttgc tattcgggcg gaacagaagc tacagccatg tttccaatgt
     7981 taggtaaaac attagaaaat caagggttta aaatcaagat gctttcggaa gggaacaatc
     8041 cggtgtatgc catcaagttt tctaaaaacg agcctgccat catagcattt tccaaaactt
     8101 ttgaggatga ttttaatccc gaaagtaatt ttgccgccat catgacgtgt tcgcaagcag
     8161 acgaaggttg tccttttatt ttgggagctg aaaaaaggat tcccattact tatgatgacc
     8221 caaaagtttt tgacaacaca cctcaacaag ccgaaaaata tgaagaacgc agtgtgcaca
     8281 ttgctactga aatgatgtat gttttttcac aaattaatca atagctatgg cagtaaatca
     8341 ttgtacacct gcagtagaac gaaagaagtt aggattttta gaccgctttc taaccttgtg
     8401 gatcttcttg gctatggcag ttggtgtttc ggttggctat ttcgttccat ctagtagcgg
     8461 ttttatcaat tccttttcta gtggtactac caacataccg ttagccattg ggttaatcct
     8521 aatgatgtat ccgccattgg ccaaagtaaa atatgagcaa atgggcaaag tctttaagaa
     8581 caccaaagta cttggtgcat ccttattcct caattggatt gttggcccca tactcatgtt
     8641 tgcattagcg ctactctttc taaaaggcta tcccgaatac atgattgggt taatcttaat
     8701 tgggttagct cgatgcatcg ccatggttgt agtgtggaat gatttagccg atggaaaccg
     8761 agaatatgcc gcaggattga ttgccttaaa cagtattttt caagtgttgt tgtacagtgt
     8821 ctatgcttat gtgttcatta ctatcttgcc accctatttt ggctataccg gttttgaagt
     8881 aaacatcagc atcggacaaa tagccgaaag tgtaggcatc tatctaggca ttccgtttgc
     8941 attgggaata attagtcgct atactttaat caaactgaaa ggtgaagaat ggtttcaaac
     9001 aaaatatgtt cttttcattt cacctataac tttagtagca ctgttgttta ccatcttaat
     9061 aatgtttagc ctaaaaggag agcttattgt tcaaattcct atggatgtga ttcgtatagc
     9121 cattccgttg gtcatttatt ttgtggttat gtttgtggtt agtttttttg tcggaaagta
     9181 ttttggtgcc gattattcta aatcaacttc aattgccttt actgccaccg gaaataattt
     9241 tgaattagct attgccgttg ccattggtgt ttttggaatc aacagcggac aagcgtttgc
     9301 aggtgttatt ggtccattgg tagaagtacc cgcattaatc gctttagtca atgttgcttt
     9361 ttggtttaag aaaaaatatt ttaaaacgga agtttagtac gaagcaaaat ccataacgcc
     9421 acaaatccca taaattttgt actttagtaa aaccttttac aatacaaaaa ctgcgattat
     9481 gacacgaagt gcgtttatag ccaaccgcct tgaagaagta ctcttgaacg gaacatggat
     9541 agccaacacc aactacaagg cacaaataaa aaacattaac tggcaacaag ccaaccaaca
     9601 agtgggaacg ctcaatacta ttgcgctact cacccatcac gttaattact atttgggtgg
     9661 actattaaac gtgttcaacg gtggcgcact cgaaatcaga gatcaattta gctttgacat
     9721 gccacccatc caatctgaaa ccgactggaa aaatctctgc catgatttct tgaccaacgc
     9781 agaattattt atacaacatg tttcaaaaat tgaagaagaa caatgggata ctgtttttgt
     9841 ggatgaaaga tacggaacct attaccgaaa tatagaaggc gttattgagc actgctatta
     9901 tcatctaggg caaatttcgt tgataaaaaa gttggtttta aaatagtaga atagtttgag
     9961 tttgcaatat gtgtaagcaa aataaaaaac cacatgaagt acaaaatatt atttattatt
    10021 atatgttttc tgttttcttt ttcagtcttt tcacaaaaga gagaagaaaa agtaacattg
    10081 ggatattatt gtggatattc gggttctgga acttcggttg taatcaaatt tgctcagctt
    10141 ttatatgata aaaagtataa aactcttaaa gaattattat actcaaagat tccagcagaa
    10201 aattttcttt cggttgttat ttgtaaaagg ttagctcata aaaagataat aactttaacc
    10261 gaattagagt taaaaagaat tgacgaacta ttccattcaa aagaaaaagt tccaatttgt
    10321 ggtggttgca ctttttttga agaagtagag ttaactaaat tattaaactc caacgaagaa
    10381 gttttttcgg caatggatta tttttttgat tcttaatata tggatttgca attgtcttaa
    10441 ataatttaat cctccatcct caaccctact tcacttcctc tttctagcgg tgtatcgtgt
    10501 tcaaaacaga gcactatttc tatgcctcag aattgaaatt ctatgctttt gaattgatgt
    10561 tctatgtttc agaattgatg ttctatgcct tagaattgat gttctgtttg attgttttga
    10621 tgcaggcatt tcaatacctg cctacaatag ttttcgtttc tatgtaactg ttatatagaa
    10681 ttttaaacta ttaagctcat taaaacaatt aagcgttgtt gggttggtat agtttagata
    10741 caagcattag aaacatgttt actatagcat ttgtttctat gaaactgtga cgataaaata
    10801 ttaagtaaaa aagagaattt ttattatgac ttaataaaac aagttactga atacgcaacc
    10861 ctattgcact gcctttttct aacgcagtat cgttttcaaa atacaacaag tgatcgttat
    10921 aagtagcttc tatcaaataa acagcacctt taaaaaaaga atttaaaaca accacttgaa
    10981 gcgcaccttt tttatctaca acaagttggt gcggatacag caatatctct ttcccatcaa
    11041 gcgtaacggt gtttacatcg ccaaaaagtg aagctatgta gtggctttta gggttttgat
    11101 agaggttttg cggagtgtct tgagctagta gtgtgccgtt ttgcatcacc aatgcttcgt
    11161 cggcaaaaga caatacatct gagctgtcat gtgtggctac tatggtggta atttgttttt
    11221 cttttaggta agcaaagagt tttcttcgca gggaattttt cctgaaatta tcaatatgac
    11281 taaaaggttc gtctagcagc aacacttcgg gttctaacgc caatactcgt gccaatgcga
    11341 tgcgttgcat ttgtcctccg ctaaggtatt tggcttttac atgagcaagc tcactcattt
    11401 ctacaatgtc gaggagttcg gctatgcgtt ggtttttttt atctttatag atgttggaca
    11461 aatatttgcc cacattctcg gctacggtga tgaacggcat gaggtcaaaa tcttgtgcca
    11521 ggtatttcat ataatccatt cctggaatca gatggtattt tggacctaac acttcggtgt
    11581 cgttccaata gatttgtcca ctgtttaggt catgcaagcc atagataagt tttaataacg
    11641 tgcttttccc acaaccgctt tcgccaatta tagccaatgt tttcccttta gtcaacgaaa
    11701 aatcaacatt tttaagtgta ggttgatcta aataagaaaa cgaaatgttt tgtagttgta
    11761 acataacttt aatgaaatac aaagttgaat aaacttttag atattaaaaa atttaattta
    11821 aattacttaa aattaaaata ttaaacattg attttgaata aaataaaata taattttgtg
    11881 ctaaaatata cagtatgggt ttgaaaactt ttattagttt gtttacattt attacggcat
    11941 tctcttttgc tcaagttgga attggaacta cttcacccca ttcacagtta gaaattgcat
    12001 caagtaatca agttacgcct gccaatactg atggagtatt aattcctaaa atcaatacat
    12061 ttcctgctac taaccctacg gcattacaac aaggaatgct tgtctattta accacaactg
    12121 ctacttttgc tagcacaagt cgttcgcctg gattttatta ttgggataat acaacaacca
    12181 actggatagg aattcaaacc aatccgactt caaattggaa tctattgggt aatgcagcaa
    12241 ctagtattag tacaaatttt attggcacta ccgataacac tgatttagtt attaaaagaa
    12301 ataacattag agcgggttat attggaaacc caagtacatc accatttaca aatgccaaca
    12361 catcttttgg tgctaactca ttgcttaacc ctacaattac aggagttgtt ggcgaaggaa
    12421 ctagaaattc tgcttttggt gttaatgcta tgcctggttt aactactggc ggtttgaata
    12481 ctggtattgg tgaatatact cttttttctg ccactacagg aagtgctaat actgctgtag
    12541 gttctgcagc tttgtattca gtatcaacag ggcgatttaa tgttgctgtt ggaagaaacg
    12601 cattggtgac ttcaaatgga agtaacaata ttggaattgg aacatctgct ttaaacggaa
    12661 attcttcggg aaacaataat attgccattg gtacatcgtc ctttagactg aatagtactg
    12721 gaagtaataa tctagcactt ggaaatcaag caggttataa tgaaactggc agcaataaat
    12781 tatacataga aatgtcaaca gcagtaagcg ggacagattc aagtgaaaac aatgcattaa
    12841 tttatggcga atttgacaat agaattgtta gaacaaatgg tcaacttcaa ataggaaatc
    12901 caacgacatc tggatatact tttccaacaa cacgaggaac aaataatcaa attcttcaaa
    12961 ccaatgcaac gggaacactt tcttgggtca attcgataaa taacttttcg ttagttagag
    13021 caaatcttac agctaatcaa gttgtaaacg caacaggttg gcaaaaaata acctttaaca
    13081 ctaccctttt tgattctggc tccgagtttg taacagcaac aaaccgattt gttgccaaca
    13141 aaaatggtta ttatgaaatc aatgctggtt ttcatacctt caataaaaat gatacagaac
    13201 aatatgcaat agctgtttat aaaaatgggg ttgaatatca agaaacagca gcgcaccatt
    13261 atggagacaa actaatttct agaacaatta attgtactat cagtttaaat gtaaatgact
    13321 atattgaaat atacatgttt aatggaaatg cacccactac tgtagatgga tttacaggta
    13381 aaacttattt tgaagtaaaa caaatacgtt aaaaaaataa gagcgatatt ctcaaatatc
    13441 gagaaaccgc tcttaccaac ctaaccaata agtttatttt taatcaatat atttttcaaa
    13501 agtttttgta tcgactcttg cttttaaatc gtgtaacaaa ttatataaac caaagaaagt
    13561 acggttgata tataagaaat gcttggagcc tcggttacca ttcatcttgc gaagttgggt
    13621 atcttttgaa aactcttcac ccatttgagc aatctttcca aaaaattctt catccgaaaa
    13681 atcaaaataa ttgccgtgaa atggcagtgt aaatagactt aacaaatcat gaaacatctt
    13741 ggtaaaatat tctatttcct tgggtgaatc gtcatttctc agaatttcta attcgtatag
    13801 tttatccgta aacaatttag gattattgat aacttttgga tctgctaatt caaaataggg
    13861 aacataaaac tcttcgggaa cgtgtttgat gcaaccaaaa tcgatggcga ctaatttctc
    13921 atttttatca ataataaaat ttccaggatg tggatcagca tgcacttgtt ttaaataatg
    13981 catttgatac atataaaaat cccaaagtgc ttgaccgagt ttatcgccca attcacgatt
    14041 tgaattgtgg gccgtaaact ccgaaaggtg ttcgccttct atccactcca ttgtcaatat
    14101 tttttcagaa gaaaattcag gaaaatattc aggaaaccga agattctcaa tttttttaca
    14161 ccattcggaa acgtcttgac tttgttttaa ttcgagtaca taatccgttt cttcgagtag
    14221 tttgtattct acttctttaa aatatttttc cgaatcttta ccacgcaggt tgaacattcg
    14281 agtagctata ggttttacca aagctaaatc ggaactaatg ctatcggcaa caccaggata
    14341 ttgaattttt actgccaatg tttttccatc tttagttgct ttgtgcacct gacctatact
    14401 tgcagcatta actgcatcag gtgtaaatgt gtcgtagagt tcttcgggat attttcctaa
    14461 atatttttta aaagttttct ttactaaagg tgcagaaagt ggtggtactg aaaactgcga
    14521 caaagagaat ttatcaacgt aggcttgtgg catgatgctt ttgtccatac tcaacatttg
    14581 agctactttt agagcacttc cttttaagtt ttttaaacca tcataaatgt cttcggcatt
    14641 attttcgttc aatttatcac gattcaactc tggatttact attttttcgc cataataagc
    14701 cacatagttt cctcctattt tgatgcccgt tttcagcaat tgactggctc gttctatctt
    14761 tcctgttgga attttatcta gtgttttcat ggttaattat attttttctt tccacaaaaa
    14821 ttttcctaag tcaaacaagt tttcgaattg tttagtatcc cataagctca tcacagtggt
    14881 aactgatttt tcaatcaata catcggtttt ttcaaatcct ctggaagtat cgtctaacca
    14941 aaattttaat aaaaataaaa attgtagcca agcaccttca gcataggtag gttctttaat
    15001 tttgttcaac ttaccaaaaa ctggattggc atctatgcca attatttctt taacaaaatc
    15061 tttgaaatgt attcggaaac gttttaaatc ttttagattt ttcaacctgt ttctgttttc
    15121 ttttaaataa aatataatat aggttctgtt taacgtcaaa atttcaaata atgtaaaata
    15181 caatgtcaat agtttgtctt tgtcgctaaa tgaggcgtaa tttttatcgt tttgaatagt
    15241 tgtcaatgca ttttcaaaaa atttttccca aatggtttcc tttaacgaat caaaagagcc
    15301 gaagaaagaa taaaaatcac tttcttctat tgaatgcttt ttacaaaaga catagacgtt
    15361 ctttggttct tcattatttt caagaacttc attcataaaa tgagtaatga ttaatgtttc
    15421 atcaataatt gctgttttta ctttcgcttt tttagttgtt gccataacgt tttgattagt
    15481 aatgtaaaaa tacaaccgtt tcatgaattt aaagattaac taaagacaaa tttttgttaa
    15541 agttctttca aaaaaaagcc atctcaaaaa agacagcttt ctattgaata tatagtttga
    15601 aaattatttt cccgcttctt tttgagcgtc ttgaaccatt ttatcgtttg ctgtgatagc
    15661 aaattctact cttcggtttt tacttctacc atctacagtt tcattacttg caattggatc
    15721 ggcaatacct aagcctgtcg ttacaaatcg agttgatgat aaacctttac ctaccaaata
    15781 tgatttaacc gaagcagctc tttctcctga taattttaaa ttgtattcag cagcacctgt
    15841 gctatcagta tagccataaa tttgaatatc agtatcagga tattgattaa aaacagggat
    15901 aagtttatct aaattagctt tggccgtagc agttaaagta gatttattag tatcaaaacg
    15961 aaccgcattt tcgcccaaaa ccaatttaat tccttctcct actcgctcca cttcagcacc
    16021 aggcaaagcc gtttcaattt ctctggcttg tttgtccatt ttattaccaa taacaccacc
    16081 ggcaacacca ccaacaacac cgccaagaac agcacctagc gcaccatttc cgccttttcc
    16141 tatgttattc ccaagaactc caccaataat ggctccggca gcagtcccta tagctgcgcc
    16201 acgttgtgtt ttgttagtgt tttttacggc ttcacatcct gtaaatacgt tacctattag
    16261 aaatgcacta acaagtatta aagttatttt tttcatgata tttagtttag tttttgaaat
    16321 tgatatacta catcggtcat ttttcctgca acatctattc gatcaatcaa ttgaaatgat
    16381 gattcggttt ggttggctac tctcaagata tatcctgatt ttactgtctt tgcttttgct
    16441 tcgtctaaaa ttttcataac aaaatttcct tccttattga tgtaccatgt aattggtgta
    16501 gaataagacg tacaacttgg actgtttaat gcaaagtttc ctttgttatt attggataca
    16561 aatttccaag tacttccaat aaaacattta gaatcagcca aatcaaagga catcacttta
    16621 atgacatctg aacctggata agaaacacta gtaatattcc aatttccttt aatagctact
    16681 tctgatttaa tatctaattt agtattagta accgatttcg acttgcagct tatgatagca
    16741 aacgaaaaaa taaataataa aataagtttt ctcatgtttt tataattaat atttctaata
    16801 caaagataag agcattttta atttagattt tataaaatta tgtaaaaatg ttgtagaatt
    16861 atttctacgc caatttgtca tacaatttat tattggtatt ggatttgcta aagattttgt
    16921 aacaaagttt aatttaataa ataacataat tatgagttca ggaaaaatta atgtttctgt
    16981 agaaaacatt ttccctttaa tcaaaaaatt cctttatagc gaccacgaaa tctttcttcg
    17041 tgaattaatt tccaacgcca ctgatgctac gttaaagcta aaacatctta ctagcattgg
    17101 ggaagctaaa atagaatatg gcaacccaat cattgaagta aaaattgaca aagaaggtaa
    17161 aaaactacac atcatcgatc aaggtgtggg aatgactgcc gaagaagtgg agaaatacat
    17221 caatcaaatt gctttttcgg gtgcagaaga gtttttagaa aaatataaag actctgccaa
    17281 agattcaggt attattggtc attttggtct tggtttttat tctgctttta tggtagcatc
    17341 aaaagttgaa atcatcacaa aatcgtataa agatgaacca gcagcacatt ggacatgtga
    17401 tggaagccca gaatttacgc ttgaaccatc agaaaaaact actcgtggct cggaaatcat
    17461 tcttcatatt gcagaagatt cattagaatt tttagaagaa tttaaaatcc gtgaattgtt
    17521 gacgaagtac aataaattca tgccggtgcc tattaaattt ggaacaagaa aagagaagct
    17581 tccattacca gaaggtgcag aaaaagatgc aaaaccagag gaaattgaag ttgataacat
    17641 catcaacaat ccaaacccag cttggaccaa acaacctact gaattaaaag acgaagatta
    17701 caaagaattc tacagagagt tgtatccaat gcagtttgaa gaaccattgt tcaacattca
    17761 tttaaacgta gattatcctt tcaacttgac aggaatttta tatttcccaa aaatggatgc
    17821 caatttgcaa atgcaaaaag acaaaatcca attgtatcaa aaccaagttt atgtaaccga
    17881 taatgtagaa ggaattgtac ctgaattttt aggaatgctt cgcggagtta ttgactcgcc
    17941 agatattcct ttgaatgttt cacgttcgta tcttcaagcc gacggaaatg taaagaaaat
    18001 tgcaaactac atcactcgaa aagttgccga taaattaaaa tcattattca cagaaaatcg
    18061 tgaagacttt gaacaaaaat ggaacgacat caaaatcgtt ttagaatatg gaatgctttc
    18121 ggaaccaaaa ttctatgaaa aggcaggtga ttttgtgtta tatccatcag taggtgacaa
    18181 atattacaca ttagccgaat taaaagaagc cataactcca acgcaaactg ataaaaacgg
    18241 aaaggttgtt gttctatatg cttcaaacaa agaagcgcaa catggttata tagaaatcgc
    18301 taaagaaaaa ggatatgaag tattgctttt ggattcgcca atcgtatcgc atttaattca
    18361 aaaattagaa gccgacaatg aaaatctaac ctttgttaga gtggactctg accatattga
    18421 caaattaatt caaaaagacg aagaacaaat ctcgaaactt tcggaagacg aagcctcaaa
    18481 actgaaaaca gttttagaag aattagtacc aaaagcaact tacacggttc aattggaagc
    18541 gatggacagt aaagcagcac cttttatcat tacacaaccc gaatttatgc gacgcatgaa
    18601 agaaatgagt caaaatggtg gcggtggaat gttcggtatg ggtaacttcc ctgaaatgta
    18661 taatttggta gttaattcta attcagaatt agcaacaact attttaaaca ccgatgacaa
    18721 atcggcacaa gaaagcttag taaaacaagc actcgacttg gcaaaaatat cgcaaggatt
    18781 attgaaaggc gaagaattga cagcttttgt aaaaagaagt tttgagttga ttaaatagta
    18841 gtaagacaga agtattaagt agtaagacct gcaattttac atttgcaggt tttttttata
    18901 aaaatttcac gaccttcgta acttgaaatc aaacttcata cttcaaaatg actttagaaa
    18961 tccaaacacc agcattactc ttctccgcta catcattaat tttacttgct tataccaatc
    19021 gttttttgac tattgcacaa atcgttcgag gtttaaaaaa aacctatgat caagagaaaa
    19081 ctaaagatat ttttttacaa ataaaaaacc tcaacttgcg cttatcattg attcgttaca
    19141 tgcagctttt tggtgtgttg tgtttgtttc tttcagtctt tgccatgcta gttctatttt
    19201 taaatctaca aacggcaggc atctatcttt ttggtgttag tttattctgt ttgctaattt
    19261 cacttggtct ttccttttgg gaaatcagta tttcagtaac tgctttacgc ctccatctgc
    19321 aagatttgat ggaataaaaa agcataaaga ataatttaag tatctttgag catagaatta
    19381 taaactacat gcaaaccaaa accgacctaa aacttgccgt attaattgat gccgacaacg
    19441 ttccttatgc caatgtcaaa gaaatgtttg aagaaattgc aaaatatggc acacctactt
    19501 tcaaacgaat ttatgctgat tggaccaaac caactgtttc aggctggaaa aaagtgttgc
    19561 tagaaaatgc gataactccc gttcaacaat acagttatac ttctggaaaa aactcctcag
    19621 atagtgcatt aattatcgat gcaatggata ttttatacac cggaaacgta gatggtttct
    19681 gcattgtttc cagcgattct gatttcacac gtttagcaac acgattgcgt gaagcaggca
    19741 tgaaagtcat cggtataggc gaaaagaaaa ccttaacacc attcatcacc gcttgcgata
    19801 aattcattta tatcgaaatt ttaaagaaag aagaagctga tgcaactaat gaaacagaca
    19861 ctaaaaaaac ctcaagaaaa ccaaaagaaa aaagcattcc gcttagtaaa attgatccaa
    19921 aaattatcaa actatttgca gatagtatcg aagatttaga agacgaaaac ggttgggcat
    19981 ttcttggcga tttaggaaac ttaatgctca aaaagaaacc cgattttgac ccaagaaact
    20041 atggcttccc aaaacttttg ccgctaatta aaagcatcaa caaatttgaa acagacatcc
    20101 gcgaaaccgg aaattcaaac atcaaacata tttatgttag aataaaataa aacaaaaaat
    20161 cccaaactca catttgggat tttctatatt acttactcaa atacatcttc cgtctcgagt
    20221 acaattcata aaattggtcg tcttttaaat catcaatgaa taaaatgctt tcgcctgtag
    20281 atttcatttc tggtccaagt gctttgttta catttttaaa tttgctgaaa gagaaaaccg
    20341 gttgttttat agcaaatcct ttcaattgcg gattaaacgt aaaatcagtt actttattga
    20401 caccaagcat cactttagta gcataattta cataaggctc gccatatgct tttgcaataa
    20461 aaggcactgt tcttgaagct cttggattgg cttcaatgat gtaaaccgta tcgtctttta
    20521 tggcaaactg aatattgatt aaaccaacag tttgcaatgc caaagcaatt ttcttggtgt
    20581 ggtcttttat ttgttgcaac acaaattcac ccaaattaaa cggtggcaat gtagcattac
    20641 tatcaccaga atgaattcca caaggttcga tgtgctccat aattccgatg atgtacacat
    20701 tttctccatc acaaatagca tcggcttcgg cttcaatagc gccgtctaag taatggtcta
    20761 acaacaattt atttccagga atatttttca acaaatcaat tacatgttcc tccaactctt
    20821 gcttattgat aacgattttc atgccttgac cacctaaaac gtaagaagga cgaactaaca
    20881 atggaaaatc caaaacatca gccaaagcac tagcttgatc ggcattttct gctacaccga
    20941 attgtggaaa aggaatattc aattcttcta aaagttctga aaatcttcct ctatcttcgg
    21001 ctaaatccaa ggcatcaaaa gaagttccga ggattttaat tccgtatttt gataattttt
    21061 cagctaattt cagagctgtt tgtccaccta attgaacgat gacaccttct ggtttttcgt
    21121 gtcggatgat atcataaatg tgttcccaga aaacaggttc aaaatataat ttatcagccg
    21181 tatcaaaatc ggtagaaacc gtttcaggat tacagttaat cattatcgtt tcataaccac
    21241 attctctagc cgctaaaacg ccgtgaacac aagaataatc gaactcaatt ccttgtccga
    21301 ttcggtttgg tcctgaacct aaaacaacta ctttttttct atcggttact atactttcat
    21361 tatctacgta tctttttcca tcggccgttt ctatttcagc ttcaaaagtg gaataataat
    21421 aaggtgtcat cgctttaaat tctgccgcac aagtatccac caatttatac acacgattga
    21481 tattcatttc ttctcgcaac ttatacactt gactttctaa acaactcatc atgtgcgcaa
    21541 tttgacggtc gccataacct ttttgctttg cttctagtaa taattcttta ggcaaagtat
    21601 caattttata ctttgaaatt tctttttcta aatgaaataa ttcttcatac tgcttcaaaa
    21661 accacatgtc aattctagta atttcgtgaa ttctgttcaa cggaattccc atcgcaatgg
    21721 catcataaat tacaaaaact ctatcccagc tggcaaacgt taatttctca ataatttgct
    21781 cgtagttttt atatcctttc ccgtctgctc ctaatccatt tcgttttatt tcaagtgatt
    21841 gtgttgcttt gtgcaaggct tcttgaaacg aacgtccaat tcccatcact tcaccaactg
    21901 atttcatttg caatcccaac gttctgtcgg aaccttcaaa tttatcaaag ttccaacgtg
    21961 ggatttttac aattacataa tctaaagttg gctcgaacaa agccgaagta gatttggtaa
    22021 tctgattgga taattcatct aaattatatc ctaacgctaa cttagtagca atttttgcaa
    22081 tcggataacc tgttgctttt gatgccaatg ctgatgaacg cgaaactctt ggatttattt
    22141 caatggcgac aatatcttct ttttcatctg gtgaaactgc aaattgaaca ttacatccac
    22201 cagcaaaatt tccgattgaa cgcatcatca aaatcgccat atcacgcatt ttttgaaatg
    22261 tagtatcgct caaagtcatt gctggagcaa ctgttatcga atcgccagta tgaattccca
    22321 ttggatccat attttcaata gaacaaatga tgacaacgtt atcgttttta tcgcgaagta
    22381 attctaattc gtattctttc cagcccaaaa gtgctttatc aatcaacact tcgtgaatag
    22441 gcgacatttc taaaccataa gtcagttttt cgtcaaattc ttcttttgtg tgtacgaaag
    22501 ctgctccagt tccaccaaga gtaaacgaag gacgaattac taatggaaat ccaaattcct
    22561 gagcaatttc ttttcctttt aagaatgatg ttgcaatttt agctggtgct gctccaactc
    22621 ctaacttggc taataattgt ttaaattgtt ctctgtcttc agtgatgttg atggcgttca
    22681 catcaacacc aatcattttt acattaaagt cgttccaaat tcctttttca tcggcttcca
    22741 aacataagtt taatgcggtt tgtccaccca ttgttggtaa aacggcatca atttgaggat
    22801 gttcttttaa aatttggatt aatgattttg tcgttaatgg caataaataa acatgatcag
    22861 ccattgaagg atcggtcata atcgttgcag gattagaatt aataagaatc acttctattc
    22921 cttcttctct taaagaacga gctgattgtg aacctgcata atcaaattca caagcttgac
    22981 ctattacgat tggaccagaa cctataatta aaaccgattt gattgaattg tcttttggca
    23041 ttgtagttag ttgtatattt agtttgtagt tgtaatgttc aaaaataatt tgtttccatt
    23101 caaaattaaa aattgctatt ttaaacttat taatatttat aaacaaatta taaaaaaagc
    23161 cgttactaat aaaagtaacg gcttgatatg tgatgaaata aattcattat tttttgtgtc
    23221 ttggttcgca agatacagtc aacttatgtc ttcctttagc acgacgacgc gcaaggactt
    23281 ttcttccatt tgcagaagcc attctgtcca taaatccgtg tttatttctt ctttttcttt
    23341 tcgatggttg aaacgttctt ttgctcattg ttcagtatct ttaaaatcgt gtataaatcg
    23401 ttgttatagg ttcttttaaa accgagtgca aatatacaaa cctttttttt tctggcaagt
    23461 acttttttaa aaatattttt aattgatttt attacctttg cacactaatt aaatttttat
    23521 catgtttcac aaaaatatca aactcgtttt agccatttta atctttgcag cagccatttg
    23581 gcaatttaca gaaagtaata ttggtaatgg gatatttcta attcttttaa ctttcatccc
    23641 tatttttctt tattataaaa acgaatatat cctattggca ttcctaaaac taagaaaaca
    23701 agattttcct ggatcaaaaa tttggttgga cagaataaaa aatccagaaa ctgctttagt
    23761 aagaaaacag caaggttact acaactattt aaacggatta atgctttcac aaaccaattt
    23821 gcatcaatct gagaagtttt ttaagaaagc tgttgaattg ggcttaagca tggatatgga
    23881 cttagccgtt gctaaattaa atttggctgg aattgccatg tctagaagac gtaaaatgga
    23941 agccactgca ttattaaacg aagcaaaaaa gcttgataaa caaaacatgc tgaaagatca
    24001 aattacgatg atgaaagatc agatgaagaa gatttagtgt tcagtcgttg tatttagtgt
    24061 tcaatataaa aaaagagctt tagaaaatct aaagctcttt ttttagttgt taactaatta
    24121 ctatatactg ctaaccaaaa tttaataacc ttctgttggg atttcgattt cgtatttctt
    24181 tttagttgga ttgagtaatt ttttgtcgcg caaccaagga ttgtgaattt ttagaatttt
    24241 ataatttatt ccttgctcta atgcgaatgt tgctaaattg ttgatcgtac tatcaacttc
    24301 aattttcttg gttggaatat tggtgtacaa ttcgtttgga aatagtgtga atccatattt
    24361 acctgaattt ttcataattt ctttcaaagc taaaattcta aaaacatacc gagaagtctc
    24421 gtctgttaaa gctaaatcat aataattagt tacttgttgc tcttcaattt ttttgttaac
    24481 tccactcatt cctccattat aagaagccgc cgccaaagtc caagaaccaa atttatcttt
    24541 ggctgccaat aaatatttac atgcagcctc tgtagatttt tctagatgat aacgttcatc
    24601 cacataatcg tttacttcca ttcctttttc tttggcagta tcgggcataa attgccaaac
    24661 accacgagca ccagcagaag atacagcatt taccaaacca ctttcaatta cggcaagata
    24721 tttaaagtca tcaggaacac catatttttg aagtattggc tcgattattg gaaatgctct
    24781 atttgccctt tttaatatta aagttgttga ggaatgaaga ttagcgttga ttaccaactc
    24841 tttatctaat cgttctttta catcggctat ttgcaaaggt gtttgttcgc cagcaaaatc
    24901 aatttcagtt ggaaaataca aacttgttcc ttcacgaact gctttttctt tgtttgcacc
    24961 cgaaatagca aaaacaaata aacctgaaat taatataatt ccaatagttg tcagaatagc
    25021 atgattcttt ttcataaggc agttttttat tttaaacata agtaaatcta caataatcag
    25081 tccaaaaagg ctaattttct aacataattt taggtaaatt ttcattgagc catttgtatt
    25141 tatttaaaat cataatatga gttccaccct tgactggaat aaattctttg atatatttta
    25201 ctggaaaaac ctcatcttca agcccatgaa tatgaataat ttcgtcatca atctctgttc
    25261 gttcccaaag aataacgctt tcaattgccc aagtcaaata ttttttatct cgaacagaaa
    25321 ggaatttttc atataatttt actctttgtt ttatataatc gccaaaagca tattttgcca
    25381 aattttctat gttttcgaat aatgctactg gaattagttt gtatgctttg gtcgttttag
    25441 cgattttcat tcttcgagga aattctttgt tagatttcac acttgaaata atgatgactt
    25501 tttttagttt taaaaattgc ttcatttctt gaaccaaaat tccgccaaat gatacaccaa
    25561 ctaaaataat gtttttgtga tgtacttgtt cagccatttt ttttgcataa tcacctaaag
    25621 tttgtttttc tttgggaaca aaccattcca aaagatggat ttcaaattgg tcattaggaa
    25681 gttgaattcg ctcaaaaata gttggacttg ccgccaaacc tggcataaag taaacatgga
    25741 ttttttccat ttttaaaatt tttataaaaa taaagatttg ttctactaat ttaaagttag
    25801 aaaggattaa ttttatattt aactttatta gttttgattt tcaacaaaat agccgtaact
    25861 ttgtgtcact ttgtgataaa actaatcaga atttaatatt tacttcaagt tttcatttgt
    25921 taatacagtt ttatagcaca tcaaagaaac atttttttaa caatttactc agaagatttt
    25981 ttaattaata agaaatcaac ttagtatttc aacttattta gaacatggaa gtacaacaaa
    26041 tgatggaaat taacgataac acttttgcaa gacaatttga aactacttta gatggaaatt
    26101 taattactgt agagtattct tttcaagaac gaaagatatt tttaactcgt attaacttgc
    26161 ctgaagattt tgaaaatgaa gattttgtag atgaattgat aaagaatatc cttgatattg
    26221 ctgaagaacg aaagcttaaa gtggttccaa tatatccgag aattaccaag ttttttagaa
    26281 aaaacaagag ttacaaagag ttgcttcctc caggaattag aatataatga tgtaaattcc
    26341 aaaaatttaa aaaaaaattc caattataaa cgtactaatt tataattgga attttttaca
    26401 tttatcacat ggatagagaa cgactaatag ccttaatact tttgtttttt gaagatatta
    26461 ccaaatatta cggttcgaca actgaaatta cagaagggat tgttactgat attgataata
    26521 ttgatgctaa tttaacgaca tggaatttat ctgagtttat tcttattcgc tctgcgtaca
    26581 gaaatactgg aaataaattc atgcttgaag gcaaaggatt gtattatgaa atggctgccg
    26641 aaaaaataat tggccttaag gaattaggcc gaaataattt tgagtttatt gaacaatata
    26701 gtgaaaatgt atacagaatt acaaaattga agtttcacta taagtattaa gttttcaaat
    26761 gaaatataaa ttatcaatcg gaaacttatc tagtgttttt atatttattt atgcaattta
    26821 ttctattcta aatcctggaa ctgaaggttg gggtttatta attggattat atttaatttt
    26881 ttttgctctt tgttatttta catttgacat aattcttcaa agattaatta agaattttta
    26941 cattctaaat attattgaaa taattccttt aatttatatt tactttttgc tgtttaaata
    27001 aaaaactaaa cttcaacacc caaaaactcc aatcttacac ttccatcttc atcaatatga
    27061 gttaacttaa tttcgtgtaa tgtatttgcc agttctggat tccatggtgt ttttactttg
    27121 acgtaatttt cggtaaatcc atgaatgtag ccttctttgt tttcgctttc aaacaaaacc
    27181 gttctgttag ttcctatttg actttcataa aaagatctgc gtttttttac agataaacct
    27241 cgtagcattt tacttctttt ggcacgaaca ttctttgcta ctattccttc catttcggct
    27301 gcttcagtgt tctctctttc ggaataggta aatacatgta aataagagat gtctaaatcg
    27361 tttaaaaaac gatatgtttc caagaaatgt tcgtcagttt ctccaggaaa accaacaatt
    27421 acatccacac ctatacaagc atgtggcatt acttcacgaa ttttattgac tctgtcaaca
    27481 tacaattctt tcatgtaacg acgtttcatt tttttcaaaa tatcgttact tccactttgc
    27541 aacggaatgt gaaaatgagg tacaaatgct cttgattttg aaactaattc aatcgtttcg
    27601 tttttcaata aattaggttc gatagaagaa attcgcaagc gttcaattcc ttctactttg
    27661 tctaattcga taactaaatc taaaaatgtg tgttcgtgtt ttttatttcc aaattcacct
    27721 tttccgtaat caccaatatt tacacccgtt aatacgattt ctttaattcc ttttgcagaa
    27781 atttctttgg cattttgcat gacgcctttc attgtatcgc ttcgagaaat tccacgagct
    27841 aatggaatcg tgcaatacgt acatttataa tcacatccat cttgaacttt caaaaatgca
    27901 cgagttctat cgccaaatga ataactacct acataaaaat cagcttcttc aatttcgcac
    27961 gaatgaactt cgcccatatc atttttggac aaatcattga tataatcggt gattttgaat
    28021 ttttcggttg caccaagaac caaatcaaca ccatcaacag cagctaaatc ttcaggtttt
    28081 aattgagcat aacaacccac agcagcaaca aaagctttct cattaacttt taatgctttc
    28141 tttacaattt gtttgaactg tttatctgca ttttcggtta ctgaacatgt atttattaca
    28201 taaatatcag ctatttcttc aaaatcgact ctatagaatc cttcattttg aaaatttctc
    28261 gcaatagttg aagtttcaga gaaatttaat ttgcatccta aagtatagaa tgctaccttt
    28321 tttttgtttt ccataagttt actcaattaa tgaaatcgtt gtcaaaaaat tgagaatgca
    28381 aagatataat aatttgaaga ttatacaatt tgaagatttg aagattagtt tattagaaaa
    28441 taccttttta aatcagctaa ttttcaaatc aaaactattc ttttctccat ttttttgttt
    28501 gttcaaagac atgttctaaa atttgaacat cttgttcagt aaattcaaca tggcgacgag
    28561 acattactac ttttgccact tcaaatgctt ttttagttaa ataagtagta aatccagaac
    28621 ttccacccca agaaaaacta ggtacaaagt ttcgtggaaa tccagagccg aaaatatttg
    28681 ctgaaacacc aacaacagtt ccggtattaa acatagtatt gattccacat tttgaatgat
    28741 cgcccatcat tagtccacaa aactgcaatc cagttttagc aaaattttca gtttcgtaac
    28801 tccacaatcg aacttcttcg taattgtttt ttaaattaga attattagaa tcggcgccaa
    28861 tattgcacca ttcacctaaa actgcatttc ctaaaaatcc atcatgacct ttattagaat
    28921 atccaaacat aacggaatta tttacttcac cacctacacg acaatgcgga ccaatagtgg
    28981 tagcaccata aattttagtt gctaatttta cttgcgcttc ttcacacaat gcaaatggac
    29041 cacgaatgac tgaaccttcc ataatttctg catttcttcc aatatatatt ggcccagttg
    29101 atgcatttaa agtcacaaac tccatctttg ctccttcttc gataaatata ttttcggatg
    29161 aaattacatt cacgcttttt ggtattattt gcgaaattct accttcagtt agtaagtcaa
    29221 aatcttcacg aatagccaca tcgtttttag caaaaatatc ccaagtattt tgaacactta
    29281 tgcattcatc atcataatat tgtatatcat aagtatcaaa atcaacttct tcttgtgcat
    29341 cattagtata gaaagcaatg acttcatcct ctttaaaaat agcttggttt ttttctaaat
    29401 tttttaccat ttcgaccaat acttcattgg gtaaaaagga ggcatttatc ataatgttat
    29461 cttccatttc taccattgga tatttctcag aaagatattc ttctgtcaaa gttgttgtag
    29521 tataacccaa atatttttcc catttttctc gaatagtcaa tatccctata cgaatttcag
    29581 caacaggacg agtgaaagta aatggaagta atgcattgcg tactgttcca tcaaaaagta
    29641 tgtaattcat atagatttta gattttagat tttagatttt tggtaaaatc aaaccttatt
    29701 attctccaaa agtacaaaag tttattgcat aaaaaaacca ttcaataaat gaatggtctt
    29761 tctaaaattt gaacacttaa aactcttatt ttgcgtgttt tgcgtatttt gttttaaatt
    29821 tatcaatacg tcctgcagta tcaataagtt tagatttacc tgtataaaaa gggtgagaag
    29881 ttctagaaat ctccattttg aaaactggat attcaacacc ttcgtgctca attgtttctt
    29941 tagtttctac agttgatttt gtaataaata catcatcgtt actcatgtct ttgaaagcta
    30001 ctaatctgta attttctggg tgaattcctt ttttcatctt gtaaatcttt tattttattg
    30061 aagtagcttg tcttgatgct ttcttattgg aaaggtatca acgtgccaca gctttttgtt
    30121 gttactattt ttaaataagt gtgcaaattt acaactattt ttgaaataac aagtataagt
    30181 gtaacttttt ttaaataaaa gctacttagc aatagtaatt gtggaattac tatatttgtt
    30241 aaattaattt aaaccaataa aaattatgga aaatcaaaca tcaccagcaa agtctgcgct
    30301 tagttacgga cttttttttg gattgattat gggtattgaa ctcattttag gctatgtatt
    30361 aaatatagac ccacaaacta acagtactta tggaattgcg attaatgtat taaactattt
    30421 agctcttcct tttattttca tatatatagc atgtaataat tacaagaaca aactaaataa
    30481 tggatttatt acttttggtc aatgcttaaa aattggagta ttagtatgtg tgattgcggc
    30541 tttagtttat ggcgtatttt atactatctt tcaacttttt gtcccagaat attttaatgg
    30601 aatgttagac aaaatggata aaattttaag agacaaaaat gctgaaatga cacaagaaca
    30661 aattgacatg gcaatatcta tgactaaaaa gtttatgtct cctgcaatag cggtcccaat
    30721 tactatagca atatttgctt ttgttggatt gttacactca ttacttgttg gagcgattgt
    30781 aaaaaaagac ccaattaatc aaagctttta agactagatg aacctatcca tagttattcc
    30841 tttattaaac gaacaggaat ctttgcccga actacatcaa tggattgtca acgtaatgac
    30901 aactcataaa tatacttatg aaatactttt tattgatgat ggtagtaaag atgattcttg
    30961 gaaaataatt gaaaatttat ccgctgaaaa tccaaatgta aaaggaattc gttttttgag
    31021 aaactatgga aaatctcaag cacttcatgc tggctttgct cgtgccaaag gcgatgtaat
    31081 tatcactatg gatgccgact tacaagacag tccagaagaa attccaggct tgtgtgaaat
    31141 gatattaaat caagaatttg atttggtttc cggatggaaa aagaaacgat atgattcggt
    31201 tgtttcaaaa aatttacctt caaaattatt caattgggca gccagaaaaa catctgatgt
    31261 tcaattgaat gattttaatt gtggtttgaa agcttacaaa aatattgttg taaaaaacat
    31321 cgaagtttca ggagaaatgc accgctatat tccagtttta gctaaaaatg caggttttgg
    31381 aaaaattgga gaaaaagtgg tgcaacatca agctcgaaaa tatggtgaat caaaatttgg
    31441 aatgagtcga tttataaacg ggtttctaga tttaatcact atttggtttt tatcaaagta
    31501 cggaaaaaga ccgatgcacc tttttggagc atttggagta ttgatgtttt taattggatt
    31561 tatttcaaca ggattaatca ttattttaaa actaataaaa ttatattcgg gttatccaac
    31621 aattttagtt actaacaatc cattgtttta cattgcgcta acaaccatga ttattggaac
    31681 ccaactgttt ttagctggat ttcttggaga aattattcta agaacaaaaa gtaacgaaga
    31741 acgttataaa atttctaaag aattataaaa agatttttta aagtgaaaaa cattgaatta
    31801 atttctcaaa aaatatttga aatcagaggt ctaaaggtaa tgctcgattt tgatttggct
    31861 gaactttatg aatcagaaac acgtatttta aaacaagccg taagaagaaa tgcaaatcgt
    31921 tttccttctg attttatgtt tgaattaaac gcctctgaga ttgaaatttt ggtatcacaa
    31981 aatgtgatac catctaaaag taaacttggc ggagctaatc cttttgcttt tacagaacaa
    32041 ggtgtagcaa tgctttcaag tgttttaaat aatgataaag ctatagaaat taacatagca
    32101 attatgagaa gttttgtagc tttgagaaaa tatgctttga cttatgaaga attaacaaaa
    32161 agaatttcgg aagtggaaaa acaattcccc gaaatttaca aagttttaaa ttatttaatg
    32221 caaaacgaca acaaagacaa aaaccaaaaa gatagaaact taattggata taaaaaagat
    32281 aaataatgca tatagaacaa tccattttag ataaagtcaa cgaatggcta acaccaattt
    32341 ttgacagcaa aacacaagat gaaataaaag aaatgatgac ttcatctcca aaagacttag
    32401 aagagagttt ctataaaaat cttgaatttg gaactggtgg aatgcgagga attatgggcg
    32461 ttggaaccaa tcgaatcaat aaatatacgt taggaaaaaa tacgcaaggt ctttcagatt
    32521 atttgaaaaa atcttttcca aatgaaaatt taaaagttgt tattgcttat gattgtcgtc
    32581 ataatagtga tactttagca aaagtagttg ccgatgtttt ttctgccaat ggtattaaag
    32641 tatatctttt ttcagaaatg cgtccaacac cagaattatc ctttgcttta aaatatttga
    32701 attgtcatgc aggcattgtt ttgaccgctt cacacaatcc acctgaatat aatggctaca
    32761 aagtgtattg gcaggacgga ggacagttgg ttccaccaca agatggagaa ataattgaag
    32821 taattgaagc cttaaattat aatgaaataa aatttgatgc taatgaaaat ctaattgaat
    32881 acatcgacaa tgcagttgac gaagcgtttg caaaatcaac ggtagaaaac gcaagtttca
    32941 acacttctga taatgcacga agaaatttaa aaatttgtta tacttcatta catggaacat
    33001 ctataaaaac agtaccctct gttttggaga aagctggtta tactgatgta aatattgtgc
    33061 cagaacaagc cattccagat ggaaattttc caaccgtttt atcgccaaat ccagaagagc
    33121 cagaagcatt gacaatggca ttggcattag ctgacaaaat taaagctgat attgtttttg
    33181 gcactgatcc tgatagcgac cgattaggtg ttgctgttag aaacaatgaa ggaaaaatga
    33241 ttttacttaa tggaaatcaa accatggtag taatgacgca ttttttatta gaacaatgga
    33301 aaaaatctgg aaaaattgac ggaaaacaat tcatcggctc aaccattgtt tctacaccta
    33361 tgatgttaga attagcgtca gcatacgatg tagaatgtaa agttggatta accggtttta
    33421 aatggattgc aaaattcatc aaagattttc ccgaattgaa atttattggc ggtggcgaag
    33481 aaagttttgg ctttatggtt ggtgatgcag ttcgcgataa agatgctgtt ggcgcaatat
    33541 tattaatgtg tgaaattgca gcacaagcaa aagaaaaagg aagttctgtt tttgagtatt
    33601 tacaaaaaat gtttgttaat tttggttatt acaaagaaca tctaatttca ttaactaaaa
    33661 aaggaataga aggtgctgac gaaatcaaac aaatgatggt cgaattaaga gaaaatccac
    33721 tttcagaaat caatggtaaa cgagtggttt gtgtggaaga ttatcaagct tcgactggaa
    33781 aagattttat gaataacgaa acttttaaat tgaccgttcc aaaatcgaat gtgctgattt
    33841 attatttaga agatggaagc aaaatttgtg ctcgaccaag tggaacggaa ccaaaaatta
    33901 agttctactt tagtgtaaac atacaaatta attctgttga agaaataccc gcagcagaaa
    33961 ctttattgaa ttcaaaaatt gaaactataa tcgcagaaat ggagttgaac taatggatga
    34021 aaatttaaaa aaaataattc cttttgcatt aaaatttaaa agtaacatta tttggaatgt
    34081 aatttacaat gtactttatg cattgttcgc tacattatct tttgttgctc tatttccttt
    34141 aatggaagtg cttttcaaaa tgaatgaagc tgtcacaaag cttcctgtat atactgggat
    34201 tgataaactt ggtgaatatg gaaaacaata tttacagttc tacattacaa aactatcaaa
    34261 tgaaaatggt caacagtatg cgctgttact aacagttgct ttagttattt ctacattttt
    34321 atttaaaaat ctttttaact atttagcctc acaacattta atgaaaatta aaaatggtgt
    34381 cttaaaagat ttaagagata aaatgttttc caaaatcatt gaattacctg tttcctatta
    34441 ttccgaaaaa agaaaaggtg atgtaatggc ccgaatgctt ggcgatgtaa acgaagtaaa
    34501 aaattccttc ttcataatac tagaactaat tattaaagaa ccacttacga taatttttgc
    34561 cattatcgct atgttaaaaa ttagtcttga gttaacactt tttgtattta tatttattcc
    34621 tatttctggt tttgtaatat caaaaattgg caaaagttta aaagcaaaat caacaaaagc
    34681 tcaacaagaa aacggctatt taatttcaat cgttgaagag actttaagcg gattaaaagt
    34741 agtgaaaagt tataattcgg agaattattt taaattgtta ttccaaaatt caataactcg
    34801 tttattaaaa ctagacaata gcattggtaa aaaaaacaat ttagcatcgc caatgagtga
    34861 atttatggga attgtagtta ttgccatttt actttggtat ggtggaaatt tagtcttggt
    34921 tgacaaaaca ttaaacggtg cattatttat cgtttattta ggtttagctt ataatatttt
    34981 gacacctgct aaatcaattt caaaagcttc ctatcaagtc aaaaatggtt tggctgctgc
    35041 tgaacgagtt tttgaagttt tggaggttga aaatttaatt acagacaatc ctaatgctgt
    35101 caaaaaaaca acttttactg ataaaatcat tttagaaaac attaacttta ggtatgaaga
    35161 tgagaacgtc ttgaaaaatt tctcacttga agttcctaaa ggcaaaaccg ttgctttggt
    35221 cggtcaatcg ggttctggga aaagtactat tgccaatttg ctgacacgct tttatgatgt
    35281 aaacgaaggc aaaataacca ttgatggcat tgacattaaa gaaattgaaa tgaaatcgtt
    35341 gcgaaattta atgggattag ttactcaaga ttctattcta tttaacgaca ccataaaagc
    35401 caacattgct cttggaaaac caaatgcaac tgacgatgaa atcatagatg ctttgaaaat
    35461 tgcaaatgct tatgaatttg tgaaagattt accagaagga attaatacga atgttggcga
    35521 tgctggaaac aaactatcgg gcggtcaaaa acaacgactt tctattgctc gcgctgtttt
    35581 gaaaaatcca ccaataatga tattagatga agcaacgtcg gctttggata ccgaaagcga
    35641 aagattggtg caaatagctt tggaaaacat gatgcaaaat cgaacttcaa tagtgattgc
    35701 gcaccgactt tctactattc aaaaagctga tgttattgtt gtcatgcata aaggacaaat
    35761 tgttgagcta ggttcgcatg aagaactatt aacaaaaaaa ggaacctatt ctaaattagt
    35821 ttctatgcag agttttgaat aaattattct gttggattat aattttctac tttccactct
    35881 ttatctaaaa gatttacttt tgaatagaaa accttatcat tactatccat ttctccgttt
    35941 ttattggtgt cttcaacagt tctaaaaaag atggaattcg tggcttctaa atatttccaa
    36001 tcaagtaact cctgaaaatc tgtacttaat ttggtaaagt tttttccatc aatagcacta
    36061 atatacaatg ctttaatgtc gctttcatcc agttttgcat ctttattagt atccatatct
    36121 tctaatacat aaaccataag ttgctgttta ttcttaacag catgatcttt taaataagtt
    36181 gccgactgaa taataacggg tttatctgtc aaagaataaa gcgtgtcttt tccgacttct
    36241 ttaaacttta tattgtttaa aaacccagta atttcatatt cgccataatt ggaaatcgta
    36301 taactaatag aattatttcg gttactagaa ccataattca ttcgctttga aaaattcaaa
    36361 tcactaattg gaaacaacaa aaaattagta ccttccatat gaataggcaa atcagctact
    36421 aaaacttttg tcgtatcaac aactatattt gcggttggtt tttgagtagt ttcataaata
    36481 acttttggtt tttcttcttc ttttttgcaa ctaacaaaaa ttcccatcga aaatagaagt
    36541 atgtagataa tgtaattttt catctttaag taatttatat caaaaataag gatttatttc
    36601 tacaatctgg ctgtcaattt aatattgaaa actcgtgtcg tcatataatt tggaattcca
    36661 tattgatttt tagtataaac atcacgaacc caagtgttag ttatagcatt ttggttatta
    36721 aacaaattga atatttcaaa accaacggat aaatctttga atttctttag ccaatttccg
    36781 tctggttttt cgctattacg ttcggttaac acgtacgaaa atccaacatc ggcacgacga
    36841 taatcccgta aacgaagttg gtaatcatac ggatcagcat aagaaggcga accacctggt
    36901 aaacccgtat tgtaaaccaa attcaagtac agtttcatgt ttggcaaatt aggcatatag
    36961 tcttgaaata aaatcccaaa tttcaatcgt tgatcggttg gtcgagcaat aaatcctttg
    37021 tcattcaaat tttcttcagt tttcatataa ccaaaactta accaagattc tgttcctgga
    37081 acaaactctc cattcaaacg catatccaat ccataagcat acgcaaccgc atcattgtca
    37141 gctcgataac gaattctaac attttccaaa gtataagtat tcacatcagt catcgattta
    37201 taataagctt cggttactaa tttgaaaggt cgattgtaca tttgaaattt ataatcgttg
    37261 ctcaaaacaa agtgaattga ttcttgtgct tttacatttg gacgaaccat tccaagcgaa
    37321 tctctcaact ctctataaga tggtggttgg tgataataac caacagctac tttaaacaac
    37381 atattctttt tccaatcggg ttttaaagaa aattgtgctc gtggactgaa aacagtttga
    37441 ttttttccgt tagtaatcga ttttccggta acttgccaat tgtgtattcg aactccaaaa
    37501 tttacccaat aatctgccga gccaatttgg tctttaaaac tccattgcgt atatcccgat
    37561 attctattaa tatcaacaaa attggttgct ctaatattct gataaggtac taatggacca
    37621 acataaggat tataaggttg atcatttgga atgtgaaatg ttggaggatt tatagaaaaa
    37681 ccagttgaat caattacttc ccactctact actctatctc taaaattttc tttagtatat
    37741 tttactcccc aatcgattat gtgttttttg tttttcaatt tgtgtgaacc tttaatttct
    37801 gcattaaaaa tcaaagcatc caaatcgttt cgagcatggt ttaattgtgt tccaatacca
    37861 cgattaaagg ttacttgacc taagttttcg gaaccaatat tagtatcaac atcgccaaga
    37921 aaataggccg caaaaatatc aaaatattct tgttcttgtg tatggtaaac agaacctata
    37981 aatttaaaag tgttgttttt atcagcttcg taaaccgttt tgaaagcacc aaaaagtgtt
    38041 tggtattgat cattttcttg accttcataa aaaatttgta atgcaatcgg ctcgtcaatc
    38101 gttccaaagt tagtttggcg agttaatggt tgataattgt atttattttt ggatgcattg
    38161 cctaaaaaac taaacgacca cttctcattt ggtttaaaat taactatcga ttgaacgtca
    38221 acgaaggttg gcctaaagtt agtttgtgtt tcttgactgt tgaccaaaag tgaattatct
    38281 cgataacgaa ctccagtaat gtttgaccat tttttgtttt tagaaacaaa atcggctgtc
    38341 aaacttccac caagaaaact ggcttcggca gcaagtgcaa atttcttagg atttctataa
    38401 gtgatatcta atacggaaga cattttatca ccatattttg cttgaaagcc accagcagaa
    38461 aaatctacat tttgtgccat ttctgtattg gtaaaactca aaccttcttg ttgaccagaa
    38521 cgaattaaaa acggacgata gacttcaatt tcgttaacat acactaagtt ttcatcataa
    38581 tttcctccac gaactgcata ttgtgtactc aattcgttat tggaatttac gcctggcaat
    38641 gattttaaaa cactttcgat acctgcattt gcacttggat tcaatctgat tgattcagga
    38701 gaaatagtcg ttataccttc aactcttcgt ttatcattgg taataataat ttcattcaat
    38761 tggtcggatt tatcattcat catcacatta aattcaaatt cttcatttgg ttttaattgc
    38821 attgaagcgg taatgttttt taaagctagg tgtgtaaaaa ctaaaattac tttttggttg
    38881 gcaggaattg ttatttcata aaatccattt atatcggtaa tagaagattt agattgataa
    38941 ctaacattaa cattttcaac agggatattg ttttcatcca acacaattcc agctactttt
    39001 gctgttgcgt tttgagaaaa aacagagaaa ctatttagaa gtataaaaag cgagaagagt
    39061 ataaattttg ttttcaattg attacattaa ggtttttgac ttcggtaaaa ttgtgtttca
    39121 aatatagtag aatttccaac attatcggta acaattactt ttaaatcatt ttttccttcg
    39181 gttacaattc cgtcgtcaaa agaatgtctt aatcgtttta atttactttc atattcaaat
    39241 aatatccaat ttccgttcag ataaccttca taggtgttta ttcctgataa gtcatctgaa
    39301 atggtcaact caatattttt ttggttggaa atccattttc cttcaatact ttttgcaata
    39361 gaaattgatg gcagaatagt atctttaatc aatttatatt gtcctaaagt ttttgaatat
    39421 atagagaacg tatttttata cctttttgtt gtgtaatgaa tgtacttttt tccatcgtaa
    39481 tagccaataa acattttttg tctgtctttt tctgacgaaa ttgtgtcttc aatacttaat
    39541 ttaaaactat tttgtagtgc aattatatct tgatgcagat aaactgtttc attttttaca
    39601 tcaaaattta acggaaaatc ttcataaaaa gtattctctg gaaaagtaac cgtaacatta
    39661 tttaaagaaa aaatagtttc tcgtttggct tttatgaaat acttattgtt ttttgatatt
    39721 tctttaatcg ttattggctc agaagaatat acaattggaa tatttatttt taaaacattt
    39781 ccatgaaaat cggatacttc aaccttatat gttttgtttt catttgaatt tacagagatc
    39841 acgccaaggt tttcatctgt tttaagaata cttaagggat aataacgctt tgaaaataat
    39901 ttttggattc taattttttg tgttttataa acttcataat caattaatgc gttcacatat
    39961 ttgttttcgt caaatgagaa tgtatcaaac tgataaccaa aagacagctt gccatttaga
    40021 gtagttttta cttgaaaaat accgttagta ttccaagaaa cattgtcaaa atcttgaaca
    40081 aaaatcccaa aaccaatctt acctgaggct ttaatttttt ctacaataat atcgccttca
    40141 gcagtttgat cataatttaa aagcaatctt ctttttgact ggttaacact agaactgtcg
    40201 tttaatggat aaacatacaa acccatcaaa taaggttttt tagtgtcttt aattattgtg
    40261 tcaaatccaa aatacattgg atttataatt ttttctgaag ctgtatctct aatttcaaaa
    40321 tgcaagtgtg gtccatccga accaccagta tttccggaca aacctattat ttccttttgt
    40381 tttacaatta attcatttgg cattggaaaa acttcaattt catacgattt agctttatac
    40441 tgctctcttt ttatatatgc ttcaatagct tcaaatccag attgtaaatg accataaacc
    40501 gttgtgtaac cattgggatg tgtaatataa attgcttttc catagccacc atgtgttatt
    40561 ttgattctcg aaatatatcc atcagcagca gcatacacaa acaaaccttc ttttttattg
    40621 gtctttaaat caaatccagc atgaaaatga ttgggccgta attctccaaa attacccgat
    40681 aattgcaatg gaatttctaa aggcgataca aaatagtttc ttggatattc attttgccca
    40741 aatgtacatg aagttacaaa caataaaaaa acgaaaactt tcataatgta atatttaagc
    40801 taagataatg aaaagtgagt aaaaaattac attgaataaa aaaaatataa gtgattaaaa
    40861 aataactctt tatttcatta agaaataaaa aagataaaaa aaaattgcaa tactaaaata
    40921 gaatactaac tttgtgaaat aagaaatgga ttctaacatt taatgagtga aattgccgaa
    40981 atatttggtt ctgttgaaat tagagtagaa aaactattaa ataagctaaa tagtttagaa
    41041 gctaatttgc tacaaaaaca atcagaactg aacaaagcag aaaatatcat taaaaatcaa
    41101 gaaaacgaga ttgaagcatt gaaaaaagct tgcgaatcac tcaaaatggc aaattcatta
    41161 cttggcagtg aagaaaataa aagagataca aaacttaaaa taaattcatt aattagagaa
    41221 attgattact gcataacaca gctatccgat taaaaacatg ggtgaaaagc ttaaaataaa
    41281 aatatcaatt gctgatcgag tatatccact aactgtggaa acctctcagg aagaagggct
    41341 aagaagtgct tctaaaaaaa ttgatactat gattaagcag tttgaagaaa attatgctgt
    41401 aagagataaa caagatgtat tagccatgtg tgctttgcaa tttgcttctc aagtagaaca
    41461 aaaacaatta gacaagtcta tcgacggaac agaaacactt gaaaaattga aaaaaataaa
    41521 tgacttatta aaagattttg cttaaaataa aacacgttct tatacatcac attaaaacac
    41581 tgcctacatt agattttatt tggtaaactc aacactaaca aattagaatg agcaaatcgt
    41641 cgctactaaa gcatgcctct tttatcgggg aaacttgaac agtgagttag ctcaaaactt
    41701 gtctatacga gtttattcat tcacctaatg taggcttttt tatatctaaa cattaacaaa
    41761 aaaaatggaa aatattttta ttataatact cgccccaatt atcggtatcg ctataggttt
    41821 tgggattgca aaattcttag aaaaaagcaa cgtttcaaac cttattaaaa acgccaaaaa
    41881 agaagcaact tctattttaa aagaagccaa tcaagaggcc gaaaacatta aaaaagacaa
    41941 acttttacaa gcaaaagaaa aatttctaga attaaaatca gaacatgaaa aagagatttt
    42001 aagtaaagac aagaaaattt cggaagttga aaaaagagta cgcgacaaag aatcacaagt
    42061 ttctagtgag ttagcaaaag ccaaaaaagt aaacgacgat tttgaaaata aaacaaaaga
    42121 atacgaatct aaaatagaaa tacttgataa aaaacatcaa gaattagaaa agctacataa
    42181 aagtcaggta aatcaattag aagttatttc aggtttgtcc gccgaagaag ctagggaaca
    42241 attggttgaa agtctaaaag cagaagccaa aaccaaagca atggcacata ttcaagatac
    42301 cattgaagaa gccaaattaa cggcacaaca agaagccaag aaagtaatta ttaacaccat
    42361 tcaacgagtt ggaactgaag aagcggttga aaactgtgtt tcggttttta acatcgaatc
    42421 ggatgatgta aaaggcagaa ttattggtcg tgaaggaaga aatattaggg ctttagaagc
    42481 tgctacaggt gttgaaatta ttgttgacga cactccggaa gcaattatcc tttcttgttt
    42541 tgacccagtt agaagagaaa ttgctcgtct ttcattacat aaattagtta ccgacggaag
    42601 aattcatcca gcaagaattg aagaagtagt agccaaaaca accaagcaaa ttgatgaaga
    42661 aattgctgaa gttggtaaac gcactgttat cgatttagga attcatggcc tacatcctga
    42721 actgattaaa gtagttggaa gaatgaagtt ccgttcatcg tatggtcaaa atttacttca
    42781 acactcaaga gaagtggcaa agctttgtgg tattatggct gctgaactag gcttgaatgt
    42841 gaagcttgcc aaaagagctg gtttacttca cgatataggt aaagttcctg atactgaaag
    42901 tgatttgcct cacgcattac ttggtatgca atgggcagaa aaatatggtg aaaaagaaga
    42961 agtttgtaac gctattggag cccaccatga cgaaattgaa atgaaatatt taatttctcc
    43021 aattattcaa gtttgtgatg ctatttcggg cgcaagacca ggtgcaagac gtcaagtgtt
    43081 agattcatac attcaacgtt taaaagattt agaagatatt gcttttggat ttaatggtgt
    43141 aaaaaatgcc tatgctattc aagcaggaag agaattgaga gttattgtag aaagcgaaaa
    43201 agtttcagat gacatggctt caagcttatc ctttgaaatt tcacaaaaaa tacaaacaga
    43261 aatgacttat cctggtcaag taaaaattac ggttatcaga gaaacacgtg ctgtaaatat
    43321 tgcaaaatag gtacaacact aatatgtata aaaaaaggga tttctattaa cagaaatccc
    43381 ttttttatac agcaaaaatt gttcttaaat cttctgcagt cattggcttc aagtaataat
    43441 tttttacttc gtcttcaaat gttttggctt tgcttttatc agctttgtcg tcagacgaac
    43501 taacaatata aattattata tctttttcta gtttattctt aattctttta aattcctcaa
    43561 gaaattgcca accatctaaa attggcatat ttatatccag taaaattaaa tcaggtgtgc
    43621 tttctccatt ttctagtttc ttttttaatc tttcgatggc taacagtccg ttttcaaaaa
    43681 aagccggatt gacatcaata ccgttgttca ttaaaagttt tttgataata aaatgaaata
    43741 ctttatcatc atctaccaca aaaacatcgt taaatttcat tattaaaata tattttaaat
    43801 gttgtacctt tatttgtttt actatctacc gttacacttc cacccataga ttcaatttga
    43861 ttttttgtta taaaaagacc aattcctcta gcattttcat ttttatgaaa ggttttatac
    43921 atcccgaaaa gtttgtcacc atgtctagcc aaatcaattc ctaagccatt gtcttgaatt
    43981 tcaagcacca cttttccatt ttctaggtaa tgattataca ttatttcggg aacacgttct
    44041 ggatgcgaat attttattgc atttgtggta aaattaagca aaatactttc caaataggat
    44101 gggttaaaat cgatataaca ttctttaggt attttattaa aaattttaac tttattttta
    44161 ctaatatcat tattaagaat atccaaaact ttgtttaaat acatattcaa
//
LOCUS       DAOOBH010000008        41562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_1194_length_41562_cov_5.568844, whole genome shotgun sequence.
ACCESSION   DAOOBH010000008 DAOOBH010000000
VERSION     DAOOBH010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 41562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41562
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1194_length_41562_cov_5.568844"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="PK218_02050"
     CDS             complement(<1..1687)
                     /locus_tag="PK218_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW97323.1"
                     /translation="MQEGISGKIANFFINSKLTILLMIGLMIIGIYSSFLIPREEEPQ
                     INVPMADVMVGYPGASPAEVESRVIKPLEKILSNIKGVEHLHSMAMNGQGMIIVQFYV
                     GQDVERSYVKLYDELAKHENMFPQGVFKPIVKTRSIDDVPMLGLTLWSEKLNDFEIRQ
                     VAEEVTSEIEKVKDVAITKEIGGRNRQVKVILDKDKMAENGVDAMGIMQMIQANNGSS
                     QSGSIINNDQEYLVTTGQFLKSSEDVENLVVGVNANLPVYLKQVATIKDGPSSPISYV
                     AFGYGKANEKFKKNKSEYPAVTISIGKVKGADAMKISEKILDKVEHLKKTLITDDVHV
                     EVTRNYGETASDKVGELLLHLGIAIIAVTFLVILAMGWRGGLVVFFSVPLTFALTLFA
                     YYLLGYTLNRITLFALVFVVGIVVDDSIIIAENMHRHFKMKRLPFKQAAIYAINEVGN
                     PTILATFTVIAAILPMAFVSGMMGPYMSPMPIGASIAMILSLFVALTVTPYLGYHLLQ
                     EKEEQKHKEEEGLETSWVYKIYNKLERPLLESSKKRRLLVGITVVLLIGSVVMF"
     gene            complement(1734..2819)
                     /locus_tag="PK218_02055"
     CDS             complement(1734..2819)
                     /locus_tag="PK218_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW97324.1"
                     /translation="MKKIFTILSLSAILFISCGKDKKELTNTEAPIAVKVSGISADNQ
                     SEFVMASGKIEAENSANVSTRMMGYVTKINVKVGQKVNAGQLLVSINNTDLQAKKAQV
                     DASILQATAGYNNAKKDYERFVALFAQQSASQKELDDMTSRYEMAKASLEGAKQMRNE
                     VMAQFNYSNITAPFSGVVTNTFVKEGDMANPGMPLVSIEGTNQMQVTAMVSESDITAI
                     QNGMKAKVLVKSTNEEFSGTVIEVSSSAKNTGGQYLVKLNLSKVDKKVLAGMFVNVQF
                     PILNKTKETSNSTDKVVVPESALVHQGQLTGIYTIGNENTAILRWLRVGKTYGNQVEV
                     LSGLNSNEQYIVSAEGKLYNGAKVKID"
     gene            complement(2846..4159)
                     /locus_tag="PK218_02060"
     CDS             complement(2846..4159)
                     /locus_tag="PK218_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW97325.1"
                     /translation="MKQMKFILTCAIFLSSFIGFSQDTLMVSKNDLVQKVTDKNLQLK
                     ITEKSYQSAKADYNQSNALFLPNINVSYTGISTTNPLMAFGSKLNQEILTQADFNPAL
                     LNDPAKIQNFATKIEIQQPLINIDGLYERQAAKAKMEAFRLQTERTKEYLELEVSKAY
                     MQLQLAYKAVKVLEKADVTGKANLKLVENYFKQGMLQKTDLLNVQVRVNEINNQLQYA
                     KSNVQNASDYLAFLLNEDTNGKVFKPLEELENTISIESLNTEISENRKDIQAMNKSSE
                     AYKKMMQSSKMGFLPKLNAFGSYELYDDKFLQTDAKGYLVGAQLSWNVFDGYKSIGKY
                     DKAKADFQKAEVETEQYKKQSQLELNKTNRQLKDAENKVNLSQLAFEQSQEAFRIRQN
                     RFAQGLEKTTDLLQSETQMIQKELEHLQAVFEYNFTKQYLQFLTK"
     gene            complement(4294..5889)
                     /locus_tag="PK218_02065"
     CDS             complement(4294..5889)
                     /locus_tag="PK218_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97326.1"
                     /translation="MKKHYYLLLIVFNFTFSQSTFDYQLEINPVTIPNFPGIHSYAFG
                     QNNGKWLIIGGRVDGIHARQPFNAFPASNNNTNIYVVDINTQQFWTASTNSLSTSIKE
                     QLQASNFNFYQDNSSLYIIGGYAFSATANDHITFDKLTSIDVPNLIDAIINGNPIQSY
                     FKQIQNSNFAITGGQLGKIDDTFYLIGGHRFDGRYNPMNNPTFTQTYSNQIRKFNIDN
                     SGNQLSISNYETITDAVHLHRRDYNLMPQIFPDGTQGYTISSGVFQINVDLPFLYPVD
                     IKSTGYFPQTNFNQYLSNYHSAKVGLYDATENRMHNLFFGGISQYYYNGTILVQDNNV
                     PFVNTISRTTRFADGSLIEYQLPVSMPSLKGASAEFILNKNLPYFENEVIKLDEITSD
                     EFVIGHIVGGIQSSTANPFTSNQTGTTSANATIYEVKLIRQPNLSVYEINGKNPFSMK
                     VVPNPSVNGKLKINFFMDYVTSIDYFVSTMDGKIIADGEISDTSIGENTMNFEIDEVS
                     NQTVVVTFIFDNKFYVSQKVLVK"
     gene            complement(6007..6210)
                     /locus_tag="PK218_02070"
     CDS             complement(6007..6210)
                     /locus_tag="PK218_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97327.1"
                     /translation="MKKLVSTLAVVAFMFTMSANASTGEPKQKKKKAKTEKTCSTEEK
                     KSCEKGEEKKGGCCASKKAEVKS"
     gene            complement(6365..7489)
                     /locus_tag="PK218_02075"
     CDS             complement(6365..7489)
                     /locus_tag="PK218_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW97328.1"
                     /translation="MKTILFIIQKEFKQIFRNKGMLPIIFILPLMQLVILSNAASFEV
                     KNIKLSYIDNDHSAQSRNLITKFEASNYFDVLKQFPTKKQANLAMEKGTVDVILEIPN
                     HFERNLLKDKKSNLSLSINAINGAAAGVTNVYIMQIISGFNQNIQTELMQFKDTDFVQ
                     PISIETIPSFWYNKTLNYKTFMVPGILVLLVTMLTLFLSSMNIVREKEVGTLEQINVT
                     PIKKHQFIIGKLFPFWVLGLVILTVGLLIAKVIFNVPILGNIFLVYGFTTIYLILILG
                     IGLFISNHTETQQQAMFIAWFFTVIFILMSGLFTPIESMPVWAQKVTLLNPIRYFVEV
                     IRMVMLKGATFTDIKIQLGVIVIYAMIVNGLAVWSYKKVN"
     gene            complement(7630..8736)
                     /locus_tag="PK218_02080"
     CDS             complement(7630..8736)
                     /locus_tag="PK218_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW97329.1"
                     /translation="MKRFIGFVKKEFYHIFRDKRSMFILFGMPIAQIMLFGFAITNEI
                     NNVDIAIFDQSKDTETQQIINKISASNYFSIQQEIQSESQIEPTFKKGKVKAVLVFEK
                     DFIKKLETSKKAKVQVITDATDPNIANTISNYITSILQNYTLEKNKNNKPIYQIQTQT
                     QLFYNPEMKSVFTFVPGVMTIILMLVSAMMTSISITREKELGTMEILLVSPLKPIQVI
                     IGKVFPYIFLSIINATVILLLGFFVFGMPIEGSLFLLAFESVLFIVCALALGILISTL
                     ADSQQTAMMISLFGLMLPVILLSGFIFPITSMPLPLQVISNIIPAKWFIIIIKAIMLK
                     GASLAIIWKETLILVFMTLLFIGLSIKKYKIRLE"
     gene            complement(8776..9510)
                     /locus_tag="PK218_02085"
     CDS             complement(8776..9510)
                     /locus_tag="PK218_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW97330.1"
                     /translation="MKQEKIIQVENLTKKFGDFTAVKGITFDVYRGEIFGFLGANGAG
                     KTTAMKMLIGISNPTSGKAIVAGFDVLTQREMVKKSIGYMSQKFSLYDDLTIKENITF
                     FGGIYSLSRKQIKEKTIQLVQELEMQDVADNLVASLPLGWKQKLSFSVALLHEPKIVF
                     LDEPTGGVDPITRRQFWEMIYTQAYKGTTIFVTTHYMDEAEYCDRVSIMVDGVIEALD
                     TPKKLKEQFKVDSMNDVFLKLARNIE"
     gene            complement(9634..10530)
                     /locus_tag="PK218_02090"
     CDS             complement(9634..10530)
                     /locus_tag="PK218_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW97331.1"
                     /translation="MSISVQNISKSYKSIQAIDDVSFEVNEGELFGLIGPDGAGKTTI
                     FRILTTLLLANEGKAKVLEYDVKQDYKAIRNSVGYMPGKFSLYQDLTVEENLTFFATI
                     FGTTIEENYDLIEDIYVQIEPFKKRRAGALSGGMKQKLALCCALIHAPKVLFLDEPTT
                     GVDPVSRKEFWQMLKRLQQKGIIILVSTPYMDEAALCDRIALIQKGKILKIDSPSNII
                     SKYDKTIYDIQSNNTHGLIHDLKNYSSNYSVYAFGEFIHYIDKNPDFNSNDLKKYLEN
                     KGHAEIIIKNATTTIEDVFMDL"
     gene            complement(10554..10922)
                     /locus_tag="PK218_02095"
     CDS             complement(10554..10922)
                     /locus_tag="PK218_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97332.1"
                     /translation="MKKIIAIASIFIMIGCKTTQINSKSITMNLKSLHTETKAVQTNV
                     LFEPKDGKVISLQIAKNEQLAEHITKVPALLVCVSGSGSYGDESGRKLSLKSGDYVNI
                     EPNVKHWVDAFEESNFLLIK"
     gene            complement(10944..11816)
                     /locus_tag="PK218_02100"
     CDS             complement(10944..11816)
                     /locus_tag="PK218_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPW97333.1"
                     /translation="MKNILIILIALTLFSCNKSDNEADAFGNFEATEITISAEANGKI
                     EFLNLEEGDVLQENAIVGQIDTIQLYLNKQQLLASKNTIYSKSTNVLSQRNVLNEQLK
                     TTLIEQKRIQNMFSESAATKRQVDEVNGKVSVLKQQMQSVETQNAPIINEVKSIDVQI
                     EKINDQLKKSKIINPVSGTVLAKYAEPNEITAFGKPLYKIANLNEMTLRVYFSETQLA
                     SIKVGQEVNVTIDDKDGTKPYKGKISWISSSAEFTPKIIQTKEERVNLVYAVKVIVKN
                     DGALKIGMPAEVKL"
     gene            complement(11869..13119)
                     /locus_tag="PK218_02105"
     CDS             complement(11869..13119)
                     /locus_tag="PK218_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW97334.1"
                     /translation="MRNLIIILFLFFGTFLQAQQKLTLEECYNLVDKNYPLAKQSQLL
                     DQKITFETDALQKAKLPKIDVNAQATYQSEVTQLPISLPNISVNPANKDQYRATLDVN
                     QLLYNGGLIDANMQLKQMQTKVQQQQIAVNLYPLKSKINYHFMSVLLLQEQKNLLISK
                     RNLLLEKIKEVQSAVKFGALLPSSEQVLEAEILKIKQALTENDFQRIKEIQNLAALTS
                     TVISNDVEFVQPITTINTNGNRPELELFKLQNQQIEQSKNILSKSNLPKLNAFGQAGY
                     GNPGLNMLNNSFETFYMVGLKMNWNVFDWHKSKSEKQALDVAKQVVATEKESFELNNE
                     MQLKELETEIQKMQKILETDAEIIALREKIIKVSDSQLKNGVITTSEYIIEVTNLFDA
                     KTNEKIHQTQLELTKVNYQINKGI"
     gene            complement(13116..13724)
                     /locus_tag="PK218_02110"
     CDS             complement(13116..13724)
                     /locus_tag="PK218_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW97335.1"
                     /translation="MKKLESNTEELIFASAIKIFHKKGLAGARMQEIADEAGINKAML
                     HYYFRSKQLLFEAVFKKAFMQLAPQIHQVLNSEDSLFEKIINFTDKYISFVMENRFLP
                     TFIIQELNNNPDFANQFFSQAEFPKSTRFLIQIEEGIQNGTIKNVNPKQLLIDMFSLA
                     IFPFIGAPLLQKITNVNESAYNNLLIERKKHIASMLINSIKK"
     gene            complement(13795..13986)
                     /locus_tag="PK218_02115"
     CDS             complement(13795..13986)
                     /locus_tag="PK218_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HPW97336.1"
                     /translation="MSKKGYIITGIGIVVGLIAGYAYYYFVGCSSGTCAITSKPLNST
                     LYGGLMGGLLFNMFVKEKK"
     gene            complement(13986..14288)
                     /gene="trxA"
                     /locus_tag="PK218_02120"
     CDS             complement(13986..14288)
                     /gene="trxA"
                     /locus_tag="PK218_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPW97337.1"
                     /translation="MNSSFQNIINSDKPVLIDFFATWCGPCKMLAPILKEVKDSLGEK
                     ISIIKIDVDKNQDLASMQQVRGVPTMILYQNGKQLWRQSGVLSKEELINVILEKIN"
     gene            complement(14281..14484)
                     /locus_tag="PK218_02125"
     CDS             complement(14281..14484)
                     /locus_tag="PK218_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97338.1"
                     /translation="MKKLLFTNWHFMRFFRIALALFLFYNAYITHEWFFIVFGIFFLI
                     QAIFNLGCSPNGCNVSYKSTKNE"
     gene            complement(14484..15890)
                     /locus_tag="PK218_02130"
     CDS             complement(14484..15890)
                     /locus_tag="PK218_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPW97339.1"
                     /translation="MKIEQIYTGCLAQGAYYIESNGEVAIIDPLREVQPYVSKATAAG
                     AKIKYIFETHFHADFVSGHVTLAEKTGAPIVFGPNANPSFKAHIAKDGEVFQLGDITI
                     TLLHTPGHTMESSCYLLKDKNGKDYALFSGDTLFLGDVGRPDLAQKAAHMTQEQLAGL
                     LFDSLRTKVMTLADDVIVYPAHGAGSACGKNLSKETVGTIGEQKATNYALRANMTKEE
                     FVKEVTDGLLPPPAYFPMNVKLNKEGYENVETIISEAKAFDAKTFELVANETDALILD
                     VRHQDDFEKGHIPKSIFIGIDGGFAPWVGALILDYKQPILLVTPEGREEETITRLARV
                     GYDNTIGYLEGSFEAWKNAGLEYDTLESVSAEKLEEKINENAKVFDVRKPGEYTAEHI
                     VDVPSTPLDFINDHLAEFPKEEEFYLHCAGGYRSVIAASILKARGYHNVINVLGGFSA
                     IKNTGIKTTNYVCPSTLK"
     gene            complement(15966..16769)
                     /locus_tag="PK218_02135"
     CDS             complement(15966..16769)
                     /locus_tag="PK218_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPW97340.1"
                     /translation="METSLIIGNIFAVLVGISLGIIGSGGSILSVPIFTYIMSVEPVL
                     ATAYSLFAVGSTALFGGIQKANQKLVDFKKVFLFGIPTVIAVFITRKYIMPSIPDVVF
                     DTDLFTLHKSVLIMLVFAVVMILAAIRMIKPLKESELILDGKLNYGSIFFQGISIGLV
                     AGFVGAGGGFLIIPALLLLAKTPMKKAIGTSLFIVAIQSLIGFLGDLSSNVEIDWKML
                     LTFTACSIVGVIIGNLISKKITATKLKIGFGYFVLTMGFYIIIKELFLK"
     gene            complement(16829..17461)
                     /locus_tag="PK218_02140"
     CDS             complement(16829..17461)
                     /locus_tag="PK218_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPW97341.1"
                     /translation="MHTILKENYGFVFEDKLIDEIAQVATLKDFKEGDVLIDFGDYIK
                     KIPLLISGAIKILREDFDEGELLLYFIEKGETCAMTMACCLGETKSEIRAIAENKGKV
                     VMIPVNFMEEWLGKYKSWRNFVFNSYNNRLKEMLAAIDSLAFLNMDDRLMNYLLEKSK
                     INSSKEIKSTHQEIAYDLHTSRVVISRLLKALENQGQIKLHRAIIEIVKS"
     gene            17615..19168
                     /locus_tag="PK218_02145"
     CDS             17615..19168
                     /locus_tag="PK218_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HPW97342.1"
                     /translation="MEPIKILWVDDEIDLLKPHILFLEKKNYNVTTCNNGRDAIDVFE
                     EGNFDIVFLDENMPGMSGLETLSEMKEKKSSTPVIMITKSEEEYIMEEAIGSKIADYL
                     IKPVNPNQILLSLKKNLDHSRLVSEKTTLDYQKEFRKIAMDMAMVNSYEDWIELYKKL
                     MFWELELENINDQAMFEILESQKVEANVQFGKFIERNYEDWFEPKADKPVQSHNLFRE
                     LVVPELVKKEKPVLFVVIDNLRYDQWKVMENVVSNFYKLEKEVPYYAILPTATQYARN
                     AIFSGMLPSEMEKQFPQYWKNDVEDGGKNLYEAEFLSAHLKRLGLNIKEDYFKITNLQ
                     SGKKLAENFKGLKDNNLVTIVYNFVDMLSHAKTEMDVVKELASDDKAYRSLTLSWFKN
                     SPLLEIIQQAQKLGFKLIITTDHGTINVKNPSKVIGDKNTSLNLRYKTGRSLTYEDKE
                     VYAVKDPKKIGLPTINMSSSYIFAKNDFFLAYVNNYNHYVSYYRNTYQHGGISLEEMI
                     VPFLVFNPK"
     gene            19243..19650
                     /gene="tsaE"
                     /locus_tag="PK218_02150"
     CDS             19243..19650
                     /gene="tsaE"
                     /locus_tag="PK218_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPW97343.1"
                     /translation="MEIQFSIEEIQQVARNILSENPKKVILFHGEMGAGKTTLIKTLA
                     RELGVTGATSSPTFSLVNEYKITSNEFVYHFDVYRLKNESEALDFGIDDYLYSGNWCF
                     IEWADKIPNLLPDAYSEVFISILPDGKRKVVLQ"
     gene            19696..20889
                     /locus_tag="PK218_02155"
     CDS             19696..20889
                     /locus_tag="PK218_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPW97344.1"
                     /translation="MATTPFTKQQLLPQEEMLEIIRHKSELFIGIPKETSYQERRICL
                     TPDAVNSLCSHGHKIMIEAGAGLSSSYSDKDYSDAGATITNDTKKVFNCPIILKVEPM
                     SMEEIGLLNMNAIVISAIQLKTRKKEYFEALSKKKVTALAFEYIKDEDGSYPAVKSLS
                     EIAGTASILIAAELMINNEYGNGLLLGNITGVPPTDVLILGAGTVGEFAARTALGLGA
                     TVKVFDNSITKLRRLQNNLNQRVFTSTIQPKTLLKALRRTDVVIGAMRGKDRCPVVVT
                     ETMVEHMKKGAVIVDVSIDTGGCFETSEVTTHEHPTFIKSNVIHYCVPNIPSRYSKTA
                     SLSISNIITPYLLQIAEDGGLEHAIRCSKGLKNGVYLYHGILTNKAIGDWFNLPDNDI
                     NLIVF"
     gene            21121..21495
                     /locus_tag="PK218_02160"
     CDS             21121..21495
                     /locus_tag="PK218_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HPW97345.1"
                     /translation="MKFYQRFAYYLVGFVIGMFFVAMVWSGKDTRCNYFPNARVLNDL
                     RNKPFHYDTEASRKLSEDWVDSIDVKNTLQYGDVDFDKSNIPDEGGKRYIVEGKTTKD
                     VEITLEIINYESKAVLKNIIRK"
     gene            complement(21560..22255)
                     /locus_tag="PK218_02165"
     CDS             complement(21560..22255)
                     /locus_tag="PK218_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lipl32 family lipoprotein"
                     /protein_id="HPW97346.1"
                     /translation="MKKIILSSAICIAMGLTATAQKLDKFGADLAKKSMMGKEIRVPY
                     TDMTSYFGYIKPGAAPDEVKNGKKMYYIYVWIPIAAPEIGIRMISPAPDSVKPTDKDY
                     ASADYTENAADTSNYFDTWVTLERAEGIMKIEDVAGKAKSASWTTIESNDDSSEMPAQ
                     PSGKKYNSLMRITSDTSNPSKSLVMGLYRIGFTTYKKGEVQGSFLAQMGAPIKLPGVS
                     IAVKPEDILANKR"
     gene            complement(22319..22630)
                     /locus_tag="PK218_02170"
     CDS             complement(22319..22630)
                     /locus_tag="PK218_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97347.1"
                     /translation="MKKKNQIIISIYFFAVLIILHWCFIASFDDLLYLHKKGFSPDII
                     ETLPFFLKPLYKGTPNLITLIALILFTFPAVLFSKEKSKSLLFISMSSFVLIFLLFLL
                     F"
     gene            complement(22841..23959)
                     /gene="dnaN"
                     /locus_tag="PK218_02175"
     CDS             complement(22841..23959)
                     /gene="dnaN"
                     /locus_tag="PK218_02175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPW97348.1"
                     /translation="MKFIVSSSYLLKQLQVLGSVINSSNTLPILDNFLFELDNNTLTV
                     SASDLETTMSATLEIDSSSEGSVAVPAKLLLDILKTFPEQPLTFTVEENSTIEISSNS
                     GKYAIAYAPGTEFPKAQVIEEPSSTLVPADVLATAISKTIFAAGNDDLRPVMSGVFFQ
                     FSPEGLIFVATDAHKLVKYARTDVKASEVADFIMPKKPLNILKSILGATDTEVKIDYN
                     DSNATFSFDNYVLMCRLIDGKYPNYEAVIPKENPNKLMINRNLFLSSVRRVAIFSNKT
                     THQIRLKIAGAELNISAEDIDYSNKAEERLTCDYQGDDIQIGFNSRFLSEMLTNLTSD
                     EIQLEMSMPNRAGILTPIDGLDEGETVTMLVMPVMLNS"
     gene            complement(24113..25789)
                     /gene="gldG"
                     /locus_tag="PK218_02180"
     CDS             complement(24113..25789)
                     /gene="gldG"
                     /locus_tag="PK218_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HPW97349.1"
                     /translation="MKVQLIKKTASVLLIVVGINIASHFVFKRFDLTADKRYTLSETS
                     LKIIDEVTQPLYVDVFLEGNFPGEFKKLQTETQQLLEEFKSKNPNIIFQFVNPMEDEE
                     VDKDVLFQSFMERGLTPVNVTLNDKGQQTQEVVFPWAIATYGDKSVKVPLLKNMIGAS
                     TAEKVVSSVQHLEYAFVNAFNTVSKEKQKKIAILNGNGELEDKYIADFARSVKENYYI
                     GPFTLDSVAKQPTETLKYLKKYDLVVIAKPTEVFTDAEKQVIDQYVINGGKTIWLMDQ
                     VNMEMDSLMQTGENLAFPRDLNLNDMFFKYGIRIQPDLIKDVMATPISLATGDKGSAT
                     QYTQFPWMFSPMIYPNSKNPIVSNLDGIKFEFASPIELLKNDIKKTVLLTSSQYSKLV
                     GTPTEVNLNMVEERPEQKEFEGNGNYPVAVLLEGKFHSVFENRVLAFKEASFSNSGKD
                     SKMIVISDGNVIKNQLDKNYQPLELGFDKWTNTLYANKEFMLNCVNYLLDDNGLINIR
                     SKEVDLPMLDKEKVYAQYTTSQIITVGLPLVVLLLFGVLFTILRKRKYSK"
     gene            complement(25794..26519)
                     /gene="gldF"
                     /locus_tag="PK218_02185"
     CDS             complement(25794..26519)
                     /gene="gldF"
                     /locus_tag="PK218_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HPW97350.1"
                     /translation="MKSIALREIKSFFGSPIGYLVIAIFLLLNGLFLWVFDGEYNILQ
                     SGFADLTPFFTLSPWILIFLIPAVTMRSFSDEIKQGTIELLLTKPLSVWQIVNGKFLG
                     AFLLIIFAIIPTFIYVYVISSLGLPAGNIDLGSIMGSYFGLLFLVAAYTSIGIFTSTL
                     SENQIVSFILSVFLCFLFYYGFEGIANQVKGFEDEIARIGMDYHFKSMARGVIDTRDV
                     IYFLSISFLFLTMTVYKLKALKS"
     gene            complement(26605..26901)
                     /locus_tag="PK218_02190"
     CDS             complement(26605..26901)
                     /locus_tag="PK218_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPW97351.1"
                     /translation="MFIRIVKMSFHQENISKFLKNFDLMKEKIRNSEGNRYLELYQDK
                     NNPCIFFTYSFWETEQDLENYRQSALFDEVWTFTKKLFNDKPEAWSVDKIATLK"
     gene            complement(26926..27750)
                     /locus_tag="PK218_02195"
     CDS             complement(26926..27750)
                     /locus_tag="PK218_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPW97352.1"
                     /translation="MSIITLTTDYGLKDHFVGALKGKILTEFPEAQIVDISHDIDLFN
                     ISETSYIIGAAYSSFPKGTVHLIGVDIEISKETTQHIAMQWNDHYFICADNGILSMLI
                     EKIVPQKIVAINIHDRLHTDASELDVFIMVAVHLAKGGLLNVIGKEIKSLKEATDLKL
                     IVSENQLKGFVIYIDHFGNAVTNITKKMFLEQSKGRNYEVRFKNKVIKTILAKYSDIG
                     KNKPRNLKEFESEKLAIFNDADFLEIALYKSNPKNSGSASSLLGLSFRDTVTIDFY"
     gene            27874..28824
                     /locus_tag="PK218_02200"
     CDS             27874..28824
                     /locus_tag="PK218_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPW97353.1"
                     /translation="MNERIIELHDIAPKEFWGAQDTHLETIKKYYPKLKIVARGTTLK
                     AFGEKEELDEFEKRFNRLMLHFTRYNNIDTNVIDRVIQSDSNEEQRMPNSDKILVHGI
                     GGKLIKALTPNQQKLVEYVEKNDMVFAIGPAGTGKTYTGVALAVKALKEKQVKRIILT
                     RPAVEAGENLGFLPGDMKEKLDPYMQPLYDALRDMLPPQTLEDYLLKGIIQIAPLAFM
                     RGRTLDNAFVILDEAQNTTHNQMKMFLTRMGKNAKFIITGDPGQVDLPRRTISGLKEA
                     ILVLKDIEGIGIIYLDDKDIVRHRLVKKVIDAYKSIENHD"
     gene            29007..29171
                     /locus_tag="PK218_02205"
     CDS             29007..29171
                     /locus_tag="PK218_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97354.1"
                     /translation="MKKLFLFIGLVSINFIFAQQNEIDITPNNSWLKAGITAEYQLEM
                     LAIFLHLIWV"
     gene            29162..29518
                     /locus_tag="PK218_02210"
     CDS             29162..29518
                     /locus_tag="PK218_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97355.1"
                     /translation="MGLDLRGQYLFNPNFGIGLASGYTHYFAKNDFDDFGIIPVAGFA
                     RYYFTKSGIFIGTDLGYGFLSNVDNNEGGMYLNPHLGYHNRDWNIYAFYQNTIAQNNT
                     NLQSVGVGVTYNIRFK"
     gene            29639..30586
                     /locus_tag="PK218_02215"
     CDS             29639..30586
                     /locus_tag="PK218_02215"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPW97356.1"
                     /translation="MNTITTTDFDFPNQKSVYRGKVREVYNINDELLVMIATDRLSAF
                     DVVLPKGIPYKGQILNQIATQFMQLTEDIVPNWLIATPDPNVTIGHLCTPFKVEMVIR
                     GYLSGHAAREYAAGKRTICGVAMPEGLKENDKFPEPIITPTTKAENGSHDEDISRESI
                     LENGIVSKEDYIVLEQYTRALYQRGTEIAYNRGLILVDTKYEFGKTKEGKIVLIDEIH
                     TPDSSRYFYAEDYQSRQNNGEQQKQLSKEFVRQWLIANGFQGKEGQQIPDMSEEYIEN
                     VSDRYIELYENILGEPFEKANIESIHERIRMNVMNYLNS"
     gene            30834..31292
                     /locus_tag="PK218_02220"
     CDS             30834..31292
                     /locus_tag="PK218_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97357.1"
                     /translation="MKTIILIGMVSLGFLNQNHAASAEQTSFINQSDNEQFLELVSLK
                     TRIKVVENTTEKNFYFDPTTVLPKAIKSLEQEIREDNLIIEGNIETQVTLSGLYSTSI
                     QKIIQEDIQVTEAKDLDVYQPLDFEAINNNPNGVIELQNKCSLKQENIKL"
     gene            31449..31823
                     /locus_tag="PK218_02225"
     CDS             31449..31823
                     /locus_tag="PK218_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPW97358.1"
                     /translation="MVKTIIHLSLALVAFANVSFASQQVTSETRTELMYSSNTPLCLA
                     IVKGDVETVKKFIEYGANVNETSNGLTPLMIAARYNKVEILKLLVDNGAHVNAKDEKG
                     YTALKYATLSNATAAIAYLKDV"
     gene            complement(31895..32986)
                     /locus_tag="PK218_02230"
     CDS             complement(31895..32986)
                     /locus_tag="PK218_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HPW97359.1"
                     /translation="MATKSILTKSSTTFLEKYLNNASPTGYESEGQKIWMEYLKPYVD
                     DFITDTYGTAVGVINPKAAFKVVIEGHSDEISWYVNYITDDGLIYVIRNGGSDHQIAP
                     SKRVNIHTKKGIVKGVFGWPAIHTRNRDKEQNASLHNIFIDCGCSTKEEVETLGVHVG
                     CVITYPDEFMILNKDKFVCRAIDNRMGGFMIAEVARLLKENKKKLPFGLYIVNSVQEE
                     IGLRGAEMITQTIKPNVAIVTDVCHDTTTPMIDKKIEGDLKMGKGPVIAYAPATQNVL
                     RELIVSTAEEKKIPFQRHATSRVTGTDTDAFAYSNGGVASALISLPLRYMHTTVEMVH
                     KDDVENVIKLIYESLLKIENNHNFSYFKK"
     gene            33064..33732
                     /locus_tag="PK218_02235"
     CDS             33064..33732
                     /locus_tag="PK218_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HPW97360.1"
                     /translation="MLTKKLLLLLLFFCSFTFGQPKKDSIRSEAPQIEVDTTFTDTIQ
                     LDEVLISKNLYEYINNKDFVLLQNRVYKVYPYAKIASERLVNLNKYIDRLKTDREKKK
                     YLKLVENYMENEFTEQLKKLSRKQGQILVKLIHRQTGQTTFGLIKEYKSGWKAFWSNN
                     TAKLFDINLKAEYKPYQVNEDYLIEYILDRAFAIGRLREQKPAFDIDMDALTKYWENG
                     KKKK"
     gene            complement(33780..35186)
                     /locus_tag="PK218_02240"
     CDS             complement(33780..35186)
                     /locus_tag="PK218_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPW97361.1"
                     /translation="MKKSLLYLLFTFLFISIVSCEDIDDNPVPSKLAINDFVWQGMNY
                     YYLWQGESPDLADNRFSSQGEYNSFLDSFETPTNLFNHLRIDNAIDRFSVIYSDYSLL
                     EGVLSGNTKNNGMDYGLKYTDASNTSVVGWVRYIIPNSDAATKNIHRGDLFYAIDGIP
                     ITGDNFRTLLASDTYTINLADYNGGAYTPNGNSVSLTKTNLAENPILINTVITQGTHN
                     IGYLMYNGFYASYDDQLNTVFGGFQSQNVTDLVLDLRYNSGGSIATATKLASMITGQF
                     TNQVFAKQQWNEKQQAFRESQDPNSLLNKFVGGLNSLNLNKVYILTTKGTASASELVI
                     NCLKPYINVVQIGDVTTGKNVGSVTLYDSPSFTKSNVNPSHKYAMQPIVLKTINNNGF
                     GDYTAGIAANAPFNVIENIGNLGVLGDPNEPLLNIAINDITASGRMRTQSAAISTRDF
                     KDAKSINPLRTEMYVEMK"
     gene            35297..35806
                     /locus_tag="PK218_02245"
     CDS             35297..35806
                     /locus_tag="PK218_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW97362.1"
                     /translation="MNQNEFMQIVSPFKDKLFRMAKRLLVSTEEAEDATQEVLVKLWN
                     KNEALDEYNSVEAMAMTMTKNYCLDQLKSKRANNLKIVHNNYTDREASLQQKVEDSDT
                     WRWVEKIMHELPEQQKLIIQMRDIEEMEFEEIGTVLEMNETAIRVALSRARKTIREKM
                     TKTHNYGIG"
     gene            35793..36239
                     /locus_tag="PK218_02250"
     CDS             35793..36239
                     /locus_tag="PK218_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97363.1"
                     /translation="MELDRIEILLEKYFEGETSIAEEKELKNYFASSNVAQHLEQYKS
                     VFGFFSQAKQEQFTAKVPLKAKKRNYVAWLSVAASVVVMFGVGFYVYNTSFASSNEDL
                     GTFDNPEMAFKETQKALDLISTNVNTGIESMSYINEFEQSKNKVFK"
     gene            36273..36797
                     /locus_tag="PK218_02255"
     CDS             36273..36797
                     /locus_tag="PK218_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HPW97364.1"
                     /translation="MKKAILTLVLLFVTNVFFGQQSLFDKFEDQDNVTAVIVNQKMFQ
                     LMSKMKVDDKETQQYVNLIKKLDNLKVFVTNNSGKAGEMKSVADKYLKSANLEELMRV
                     NQGGKSVKIYVRSGSKDTLVKELFMFVEGAPKEQTVLLSLTGEFDLNDISVLTDKMNL
                     PGGDDLKKASKGKK"
     gene            36804..37343
                     /locus_tag="PK218_02260"
     CDS             36804..37343
                     /locus_tag="PK218_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HPW97365.1"
                     /translation="MKLVYLIGFTIVMMLFSCHSEPSLQRYFVDNLENKDFVSLDVSS
                     NIINIDKAKLTSEEKDALESFEKMNILLFKMDSAKTKTKQFEIESAKVKQILKGKEYQ
                     ELMKVGSGTNAASISFVGDEDKIDEFVIYAKGKDTGFAIVRILGDDMNPNNVMSILSI
                     IKNSGIDMEQLKPLEGLMK"
     gene            complement(37397..37840)
                     /locus_tag="PK218_02265"
     CDS             complement(37397..37840)
                     /locus_tag="PK218_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013868694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HPW97366.1"
                     /translation="MWIHILVFVLTFCIMEFMAWFTHKYVMHGFLWYLHADHHKKDHD
                     SWFERNDAFFIFYAVVSIGCFLLWQREILEIGLSIGLGIFAYGVAYFIVHDIFIHQRF
                     KLFRNANNIYAKGVRRAHKMHHKHLGKTDGECFGMLFVPFKYFKK"
     gene            complement(37847..38686)
                     /locus_tag="PK218_02270"
     CDS             complement(37847..38686)
                     /locus_tag="PK218_02270"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HPW97367.1"
                     /translation="MKSIFDTVSFDCSRHVTKSYSTSFSAAVKMLSPKIRQDIYNIYG
                     FVRFADEIVDSFHEYNKEELFLLFEKDLSEALKNKISLNPILNSFQHTVHKYKIPHTM
                     IAAFMKSMKLDLHKSTYNSFEEYNEYIYGSADVVGLMCLKVFVDGDKQKFEELEHSAM
                     RLGSAFQKVNFLRDLKADFEDLNRTYFPNTDLTRLDEASKNEIIREIEADFEAGFKGI
                     VKLPLEAKFGVYTAYIYYKKLLSKLKKTPSLEIKNTRIRVSDYQKFGLLARCYFNYHL
                     NLI"
     gene            complement(38698..40155)
                     /locus_tag="PK218_02275"
     CDS             complement(38698..40155)
                     /locus_tag="PK218_02275"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HPW97368.1"
                     /translation="MKKKINIIGSGFSSLAAACYLAKEGNEVTVFEKNNTIGGRARQL
                     KKEGFTFDIGPTWYWMPDVFERFFADFDKKPSDYYELIKLAPAYQVYFGINNFITIAD
                     NLSEIVKTFENIEKGSGKKLTSFIKEAQDNYNIAIKDLVYRPGVSPLELVTLETAKKV
                     NQFFGNISNDIRKKFKNKKLVQILEFPVLFLGAKPSDTPSFYSFMNFADFGLGTWHPK
                     NGMYSVILAMEQLALELGVKITTNANVTKINVQNNKAVSITVNEIEEKADVILSGADY
                     HHSETLLDENYRVYSEKYWNKKIFAPSSLLFYVGFNKKIYNVEHHSLFFDVDFDVHAS
                     DIYDNPKWPEEPLFYASFPSKTDQNAAPKDCEAGIFLIPLAPGLEDTPELREKYFNKI
                     ISRFENITQQAVQKNIICKESFCVNDFIKEYNSYKGNAYGLANTLLQTAFLRPKLKSK
                     KVNNLYFTGQLTVPGPGVPPSLISGKLVSELITKN"
     gene            complement(40179..41075)
                     /locus_tag="PK218_02280"
     CDS             complement(40179..41075)
                     /locus_tag="PK218_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPW97369.1"
                     /translation="MNNIKSVFSIKDLENLSGIKAHTIRIWEKRYNILEPMRTDTNIR
                     LYDVANLQKLLNITLLHDHGYKISKISRFPKEQIPQIVNEIITKKSAKSHAISAFKMA
                     MMNFDQTLFFSTYNDLLNEKTFREIFFEVFIPLIEEIGILWQNNTISPAHEHFISYLI
                     KQKVLINTEKLQMQEPIKNDTVFILYLPLNEIHELGLMYLNYEILLQGYKTIFLGESV
                     PIECIKEMNVHFDNITFICYMTVEPSPSDINNYVIQLKDEFKNFEHKFWFIGRMIENI
                     DPKLLDNQYQSFHSILELTHNL"
     gene            complement(41179..41262)
                     /locus_tag="PK218_02285"
     tRNA            complement(41179..41262)
                     /locus_tag="PK218_02285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41226..41228),aa:Leu,seq:gag)
     gene            complement(41303..41375)
                     /locus_tag="PK218_02290"
     tRNA            complement(41303..41375)
                     /locus_tag="PK218_02290"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41340..41342),aa:Lys,seq:ttt)
     gene            complement(41402..41474)
                     /locus_tag="PK218_02295"
     tRNA            complement(41402..41474)
                     /locus_tag="PK218_02295"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41439..41441),aa:Lys,seq:ttt)
BASE COUNT        13752 a         6712 c         6662 g        14436 t
ORIGIN      
        1 aaaacattac tactgaacca attaataata caacggtaat accaactaaa agtcgtcttt
       61 tcttgctgct ttctaataat ggtcgttcaa gtttgttgta aattttatac acccaactgg
      121 tttctaaacc ttcttcttcc ttatgttttt gttcttcctt ttcttgaagt aaatgatacc
      181 ctaaataagg agttactgtt aatgccacaa acaacgaaag tatcatcgca atggaagcac
      241 caattggcat tggactcata tatggtccca tcattccaga tacaaaagcc atgggaagaa
      301 ttgcggcaat aacggtaaat gttgccagta tagtaggatt tccaacttca tttatagcat
      361 aaatcgcagc ttgtttaaat ggtaatcgtt tcatcttgaa atgcctatgc atgttttccg
      421 caatgataat actatcatca accacaattc cgacgacaaa tactaaggca aaaagtgtaa
      481 ttctatttag cgtataacct aatagataat aagcaaataa ggttaatgca aacgttaatg
      541 gtaccgaaaa gaacactacc aatccgccac gccagcccat agccaaaatt actaaaaaag
      601 taacggctat gattgcaatt cctaagtgaa gtagcaactc acctacttta tcagaagctg
      661 tttcaccata atttcgagtc acttctacat gaacatcatc tgtgattaaa gttttcttta
      721 aatgctcaac tttgtctaaa attttctccg aaattttcat ggcatcagca ccttttactt
      781 ttccgataga aatagtaacg gcaggatatt ctgatttatt ttttttgaat ttttcattgg
      841 cttttccgta accaaatgcc acataactta ttggagatga tggtccgtct ttaatggtcg
      901 ctacttgttt taaataaact ggtaaattag cattgacacc aactactaga ttttctacat
      961 cttctgatga tttaagaaat tgacctgttg taactaaata ttcttgatca ttatttatga
     1021 tgcttccaga ttgtgaactg ccgttattgg cttgaatcat ttgcataatt cccattgcat
     1081 caaccccatt ttcagccatt ttatctttgt ccaaaatcac ttttacttga cgatttcttc
     1141 caccgatttc tttggtaata gcaacatctt ttactttttc aatttccgaa gttacttctt
     1201 cagctacttg acggatttca aaatcattca atttttcact ccaaagtgtt aacccaagca
     1261 taggaacatc atcaatagaa cgtgttttta ctataggttt gaatacacct tgtggaaaca
     1321 tattttcatg ttttgccaat tcgtcgtata atttcacata cgaacgttct acatcttgac
     1381 caacataaaa ctgaacgata atcatgcctt gtccgttcat ggccatacta tgcaaatgtt
     1441 ctacaccttt gatgtttgac aatatttttt cgagtggttt tatcacacga ctttcgactt
     1501 ctgccggact tgctccagga taaccaacca ttacatcagc cattggaaca ttaatttgtg
     1561 gttcttcttc tcttggaatt aagaacgaac tgtagatacc aatgatcatt aatccaatca
     1621 ttaacaaaat agttaattta gaattgatga aaaaattggc tattttacct gatatacctt
     1681 cttgcataat tttttaattt gaaaatttga aaatgagata atttgaaaat gtattagtca
     1741 attttcactt ttgctccgtt gtataatttt ccttcagcag aaacgatgta ttgctcgttt
     1801 gaatttaatc cagacaatac ttctacttga tttccatacg tttttccgac acgcaaccat
     1861 cttaaaatag ctgtattctc atttccgatt gtgtaaattc cagttaattg accttggtga
     1921 accaatgcgc tttctggcac tactacctta tccgttgaat ttgaggtttc ttttgtttta
     1981 tttagaattg gaaattgaac gttgacaaac attcctgcta atactttttt atctacttta
     2041 cttaaattta atttcactaa gtattgtccg cccgtatttt ttgcagaact actaacttct
     2101 ataaccgttc ctgaaaattc ttcgttagtt gattttacta atacttttgc tttcattccg
     2161 ttttgaatgg ctgtaatgtc gctttcagaa accattgcgg ttacttgcat ttgattggtt
     2221 ccttcgatac ttactaatgg cattccagga ttggccatgt cgccttcttt aacgaaagta
     2281 ttggtaacta ctcctgaaaa tggcgctgta atattagaat aattgaactg tgccatgact
     2341 tcatttcgca tttgttttgc gccttccaaa cttgcttttg ccatttcata acgcgatgtc
     2401 atgtcgtcta attctttttg agaagcactt tgttgcgcaa acaacgctac aaaacgttcg
     2461 tagtcttttt tggcattatt atatcccgcg gtagcttgta aaatagaagc atctacttgt
     2521 gcttttttgg cttgtaggtc agtgttatta atgctaacca aaagttgtcc tgcatttact
     2581 ttttgtccta ctttaacatt tatctttgta acataaccca tcattctggt ggaaacattt
     2641 gcactatttt cggcttcaat ttttccgctt gccattacaa attctgactg attatctgct
     2701 gaaattccgc ttacttttac agcaattggc gcttcggtat tggttaactc ttttttatct
     2761 tttccacagg aaatgaataa aattgctgac aaagaaagga ttgtgaatat ttttttcatt
     2821 tgtatgtatt tttatttctt tatgattatt tggttaaaaa ctgtaaatat tgtttggtga
     2881 aattgtattc aaaaacggct tgcaaatgct ctaattcttt ttgtatcatt tgtgtttccg
     2941 attgaagtaa atctgttgtt ttttctaaac cttgcgcaaa acgattttga cgaattctga
     3001 atgcttcttg cgattgttcg aaagctaatt gggatagatt tactttgttt tcagcatctt
     3061 ttaattggcg attggtttta ttcaattcca attgactttg ttttttatac tgctcggttt
     3121 ctacttcagc cttttgaaaa tcagctttgg ccttgtcgta ttttccaatg gatttatagc
     3181 catcaaaaac attccaagac aattgtgctc caactaaata tccttttgca tcagtttgca
     3241 gaaatttatc atcatacaat tcataacttc caaaagcatt caattttggt aaaaatccca
     3301 tttttgacga ttgcatcatt tttttatacg cttccgaaga tttattcatt gcctgaatat
     3361 cttttctgtt ctcggaaatt tcagtgttta agctttcaat agaaatagta ttttctaatt
     3421 cctctaaagg tttaaatacc tttccgtttg tatcttcatt taataaaaat gccaaataat
     3481 cggatgcatt ttgcacgtta cttttagcat attgcaattg attattgatt tcatttacac
     3541 gcacttgaac attcaacaaa tcggtttttt gcaacattcc ttgtttaaaa taattttcca
     3601 ctaatttcaa attggctttg cccgtaacat ctgctttttc aagcactttt acggctttat
     3661 acgccaattg caattgcata taggctttgg aaacttcgag ttctaggtat tctttggtac
     3721 gctcagtttg caatcgaaaa gcttccattt ttgctttggc agcttgtctt tcatatagac
     3781 catcgatatt aatcaatggt tgttgaattt caattttagt agcaaaattt tgaatttttg
     3841 cgggatcatt taataaagct ggattaaaat cagcttgggt taaaatttct tgattcaatt
     3901 tagaaccaaa agccatcaac ggatttgtag tagaaattcc ggtatatgac acatttatat
     3961 taggcaaaaa aagtgcattc gattggttat aatcagcttt tgccgattga taactttttt
     4021 cagtaatttt taattgtaaa tttttatcgg ttactttttg cactaaatca tttttggaaa
     4081 ccatcaaagt atcttgactg aatccaataa atgagcttag aaaaatagca catgttaaaa
     4141 taaacttcat ctgtttcata attgtatttc ttaattatgt agcaaattta aggctagatt
     4201 aaccggatga cagtaacaag tgttacacta aagtcagata taaaaaaagt gctctattac
     4261 tagaacactt ttcaaccaaa acaactaaat aaattattta accagcactt tttgagatac
     4321 ataaaactta ttatcaaaaa taaaagtaac tacaaccgtt tgatttgaaa cctcatctat
     4381 ttcaaaattc attgtgttct cacctataga agtatctgaa atttctccat cggcaattat
     4441 ttttccatcc attgttgaca caaaataatc gattgaggta acataatcca taaagaaatt
     4501 gatttttagt tttccgttta cactaggatt tggaacaact ttcatagaaa atggattttt
     4561 accatttatt tcataaacac ttagattagg ttgacgtatt aattttactt cataaatggt
     4621 tgcatttgca gaagtagtgc ccgtttgatt tgaagtaaat ggattggcag ttgaactttg
     4681 aattccacca acaatatgac caattacaaa ttcatctgaa gtaatttcat ccaattttat
     4741 aacttcattt tcaaaataag gtaaattttt atttagaata aattcggcac tagcgccttt
     4801 tagtgacggc attgaaacgg gtaattggta ttctatcaaa gaaccatctg caaatcgagt
     4861 agtcctactt atggtattta caaaaggaac attattgtct tgcactaaaa ttgttccgtt
     4921 ataataatat tgactaattc caccaaaaaa caaattgtgc atacggtttt cagtggcatc
     4981 atacaaacca acttttgcac tatggtaatt actcaaatat tgattgaaat ttgtttgcgg
     5041 aaaatatcct gttgatttaa tatctaccgg atacaaaaat ggcaaatcaa catttatttg
     5101 aaaaactccc gaagaaatcg tgtaaccttg tgtgccatct gggaatattt gcggcattaa
     5161 attataatct cttcgatgca aatgaaccgc atctgtaatg gtttcataat tagaaatact
     5221 caattggttt ccagaattat caatattgaa ttttcgaatt tgattggaat aggtttgtgt
     5281 aaacgttggg ttattcattg ggttgtatct tccatcaaaa cgatgtccac caatcaaata
     5341 aaaagtatcg tcaattttac ctaattgtcc gcctgtaatg gcaaagtttg aattttgaat
     5401 ttgtttaaaa taactttgaa ttggatttcc gttaattatt gcatcaatta gattaggaac
     5461 atctattgat gttaatttat caaaagtaat atgatcattt gctgtagcag aaaaagcata
     5521 accaccaata atatataaag aactattgtc ttgataaaaa ttaaaattgg aagcctgtag
     5581 ttgttctttt atactcgttg ataaggaatt agttgaagca gtccaaaatt gctgtgtatt
     5641 aatatcaacc acataaatat ttgtattatt gtttgaagca ggaaatgcat taaacggctg
     5701 cctggcatga attccatcaa ctcttccgcc aatgattaac cattttccat tgttctgacc
     5761 aaatgcatac gaatgtattc ctgggaagtt tggaatagta actggattga tttctaactg
     5821 ataatcgaaa gttgattggg aaaaagtaaa attaaaaaca atcaacaata aatagtaatg
     5881 ttttttcata agatggattt gaatttaaca aatttaacct attaaaaaca tatagattgc
     5941 aacatttgtt acacaaaaaa agtgcttgat ttctcaaaca cctttctatg ataatttcga
     6001 aagaaattaa gacttaactt ctgctttttt tgaagcacaa catccgcctt ttttctcttc
     6061 acctttttca caagactttt tctcttctgt tgaacaagtt ttttcagttt tagctttttt
     6121 ctttttttgt tttggctcac ctgttgaagc atttgctgac attgtaaaca taaaagctac
     6181 aactgccaat gtagaaacta attttttcat tttgattatt cttatttgtg attaatttgt
     6241 aaacctattt gctaaaacaa atataatttc attattctaa atttaatgta acaaatgata
     6301 cattaaaaca aactttgctt tagctttaga attcaaatat atactttttt tttattgaaa
     6361 aaaattaatt aacttttttg taactccaca cagctaaacc attcacaatc atagcgtata
     6421 ttactattac acctaattgt atttttatat ccgtaaaagt tgctcctttg agcattacca
     6481 ttctaattac ttctacgaaa tagcgaattg gatttaataa cgttactttt tgagcccata
     6541 cgggcatgct ttcaataggt gtgaaaagtc cgctcattaa aatgaatatc acggtaaaaa
     6601 accacgcaat aaacatcgct tgctgttggg tttctgtatg attagaaata aacaaaccaa
     6661 ttcctagtat tagaattaaa tagattgtag taaatccgta aaccagaaat atatttccta
     6721 gaattggaac gttaaaaatt acttttgcaa tgagtaaacc aacagttaaa attactaatc
     6781 ctaaaaccca aaatggaaat aatttcccaa tgatgaattg gtgttttttg atgggtgtca
     6841 cgtttatttg ttctaaagtt ccgacttctt tttcacgaac tatattcatt gaggataaaa
     6901 atagggtaag catggttact aacaacacta atattcctgg aaccatgaag gttttgtaat
     6961 ttaaggtttt gttgtaccaa aaagacggaa tggtttcaat ggaaatgggt tgcacaaaat
     7021 cagtgtcttt gaattgcatc aactcggttt gaatgttttg attgaaacct gaaatgattt
     7081 gcatgatata tacattcgta actcctgctg cagcaccgtt tatcgcattg atgcttaatg
     7141 atagattgga ttttttatct tttaaaagat tacgttcaaa atgatttgga atttctaaaa
     7201 tgacatcaac ggttcctttt tccatagcta aatttgcttg ttttttagtt ggaaattgtt
     7261 tcaatacatc aaaataattt gaagcctcaa atttcgtgat taaatttcgt gattgtgctg
     7321 aatgatcatt gtctatataa gataacttaa tgttttttac ttcaaaactg gctgcgttgg
     7381 atagtatcac caattgcatt aaaggcaata taaaaatgat aggcaacatt cctttgtttc
     7441 ggaatatttg cttgaattct ttttgtatga tgaatagtat tgttttcact ttgtgagtat
     7501 taagattgga gtattaagta gtaagactat tgtgttatga aaacggattt catagccctg
     7561 atagtagtgg aaatcctttt ttgaaaaaaa agattgcaac ggatagcagg aaatagcttc
     7621 taaaaaaaat cattctaatc gtattttgta ttttttgatg cttaatccta taaatagtaa
     7681 ggtcataaag actaaaatta gggtttcttt ccaaattatg gcaaggcttg ctccttttag
     7741 catgatggct ttgatgatga tgatgaacca tttggctggt ataatgttgc tgatgacttg
     7801 taatggtaat ggcatgctcg taattgggaa aatgaatcct gataatagaa taacgggtaa
     7861 cattaatccg aataaggata tcatcattgc ggtttgttgg gaatcagcta aagtggaaat
     7921 taatattcct aatgctaagg cacaaacaat gaataagacg ctttcaaatg ctaataaaaa
     7981 cagacttcct tcgatgggca ttccgaaaac gaaaaagcct aaaagtaaga ttactgttgc
     8041 gttgatgatg gataaaaaga tgtagggaaa tacttttccg atgatgactt gtatgggttt
     8101 tagtggtgaa accaatagaa tttccatcgt tcctaattct ttttcgcgtg tgatggaaat
     8161 ggatgtcatc atggctgaaa ccaacattaa aataatagtc ataactccag gaacgaaggt
     8221 gaaaacgctt ttcatttcgg gattataaaa gagttgggtt tgcgtttgaa tttggtatat
     8281 tggtttgttg tttttgtttt tttctaatgt gtaattttga agaattgatg tgatgtaatt
     8341 ggaaatggta ttggcaatat ttggatcggt tgcatcagtg atgacttgga cttttgcttt
     8401 tttagatgtt tctaatttct tgataaaatc tttttcgaaa actaaaacgg ctttgacttt
     8461 tccttttttg aaagtgggtt cgatttgact ttctgattgt atttcttgtt gaatgctaaa
     8521 atagttagaa gcacttattt tgttgataat ttgctgggtt tcggtgtctt ttgattggtc
     8581 gaaaatggca atgtcaacgt tgtttatttc gtttgtaatg gcaaatccga atagcattat
     8641 ttgagcaatt ggcattccga aaagaatgaa catagaacgt ttatcacgaa aaatgtgata
     8701 gaattctttt tttacaaaac cgatgaatct tttcattttt taatagtaat tagtaactag
     8761 caaatagtaa ttagcttact caatgttacg tgctaatttc aaaaaaacat cattcataga
     8821 atctactttg aactgttctt ttagtttttt tggtgtgtct aaggcttcaa ttacgccatc
     8881 aaccattatg gaaactcggt cgcaatattc ggcttcgtcc atgtaatgtg tggttacgaa
     8941 tatggttgtg cctttgtagg cttgcgtgta aatcatttcc caaaattgtc ttctggttat
     9001 tgggtcaacg ccaccagttg gttcatctag aaaaacgatt tttggttcgt gtaataaggc
     9061 tactgaaaaa gataatttct gtttccaacc taatggcaat gaagctacca gattatccgc
     9121 aacgtcttgc atttctaatt cttgtactaa ttgaattgtt ttttctttta tttgcttacg
     9181 cgataagcta taaattccac caaaaaaagt gatgttttcc ttaattgtta aatcgtcata
     9241 caaagagaat ttttgactca tgtaaccaat gcttttctta accatttctc tttgtgtcaa
     9301 tacatcaaaa cctgctacaa ttgcttttcc tgaagttgga ttagaaatac ctatgagcat
     9361 tttcattgcg gttgtttttc ctgcaccatt tgcaccaaga aaaccgaaaa tttctcctct
     9421 atacacatca aaagtaattc ctttgacagc agtgaaatct ccaaattttt tggttaagtt
     9481 ttctacttgt atgatttttt cttgtttcat tttgtttttt tagacacaaa gtatcaaagt
     9541 tttttagcca caaattcgca aattattttt cttttttttg ccacagattt cacagatttt
     9601 cacagattac tgaatactaa atactgatca cttttacaaa tccataaata catcttcgat
     9661 ggttgtggtt gcatttttta ttattatttc tgcatgacct ttattttcta aatatttctt
     9721 taaatcattt gaattgaaat caggattttt atcaatataa tgaatgaatt ctccaaaagc
     9781 ataaacactg taattactgg aataattttt taaatcgtga attaatccat gcgtattatt
     9841 ggattgaatg tcataaatag tcttgtcata ttttgaaatg atgttacttg gtgaatcgat
     9901 ttttaatatt tttccttttt ggattaaggc aattctatca cacaatgcag cttcgtccat
     9961 ataaggtgtg gaaactaaaa tgataattcc tttttgttgt aaacgtttca gcatttgcca
    10021 aaattctttt cgagaaacag gatcaactcc agtagttggt tcgtctaaaa ataagacttt
    10081 tggcgcgtga attaaggcac aacataaggc taacttttgt ttcatcccgc cagataatgc
    10141 tcctgctctt cgttttttaa aaggttctat ttgaacgtaa atatcttcga ttaaatcgta
    10201 gttttcttca atggttgttc cgaaaatcgt cgcaaaaaat gtcagatttt cttctacagt
    10261 taaatcttga tacaaggaga attttcctgg catataacct actgaatttc ggatggcttt
    10321 ataatcctgt tttacatcat attctaagac ttttgctttt ccttcattgg ctaacaaaag
    10381 tgtggttaga attctgaaaa tagtagtttt tcctgctcca tctggtccaa ttaatccaaa
    10441 taattcacct tcgtttactt caaaagaaac gtcatcaatg gcttgaattg atttatagga
    10501 ttttgatatg ttttggacag aaatactcat ttttattagt tgaaagcttt ggattattta
    10561 atcaaaagaa aattactttc ttcaaatgca tctacccaat gttttacgtt tggttctata
    10621 tttacataat cacctgattt taagctcaat tttctaccac tttcatcgcc ataacttcca
    10681 cttccagaaa cgcaaactaa caaggctgga acttttgtaa tgtgttctgc taattgttcg
    10741 tttttagcaa tttgtaatga aattacttta ccatctttag gttcaaacag aacattagtt
    10801 tgaacggctt tagtttctgt atgaagactt tttaaattca tagtgattga tttagagtta
    10861 atttgagttg ttttacatcc aatcattata aatatagatg caattgcgat aatttttttc
    10921 atttttaatg tttataaatt agtttacaat tttacttctg ctggcattcc aattttcaat
    10981 gcaccatcat tttttacgat aacttttaca gcataaacta agttgactct ttcctctttt
    11041 gtttgaatga tttttggggt gaattctgcc gatgatgaaa tccatgaaat ttttcctttg
    11101 tagggttttg ttccgtcttt gtcgtcaatg gttacattta cttcttgccc aacttttata
    11161 gatgctaatt gggtttcact aaaataaacg cgtaaagtca tttcatttaa attagctatt
    11221 ttgtataagg gtttgccaaa cgctgtaatt tcatttggct cagcatattt tgctaaaact
    11281 gttcctgaaa ctggattgat aattttcgat ttttttagtt ggtcgttgat tttttctatt
    11341 tgaacatcaa ttgatttaac ttcgttaata attggtgcgt tttgggtttc tacactttgc
    11401 atttgttgtt ttaaaacgct tacttttccg tttacttcat ccacttggcg tttggttgca
    11461 gcactttcag aaaacatatt ttgtattctt ttttgctcga ttaaagtcgt ttttagttgt
    11521 tcgtttaaca cattacgttg cgataaaaca ttggttgatt tagagtaaat cgtgtttttt
    11581 gaagccagta attgttgttt attcaaatac aattgtatag tgtcaatttg accaacaata
    11641 gcattttctt gcaaaacatc tccttcttct aagtttaaga attcaatttt accgtttgct
    11701 tcagctgaaa tagttatttc tgtagcttca aaatttccga aagcatcagc ttcattatca
    11761 cttttgttac aagaaaataa ggttaatgca attagtataa ttaatatatt tttcatttga
    11821 tgataatttt tgttggaatt tggaatttta aatattgggg tttttttatt atattccttt
    11881 atttatttga taatttactt tagttaattc gagttgcgtt tgatgtattt tttcgttggt
    11941 tttcgcatca aataaattcg ttacttctat tatatattca gaagttgtga tgactccatt
    12001 tttaagttgt gaatcagaaa ctttgatgat tttttcgcgc aaagcaataa tttcagcatc
    12061 ggtttcaagg attttttgca tcttttgaat ttcagtttcc aattctttta attgcatttc
    12121 gttattcaat tcaaatgatt ctttttctgt ggcaactact tgttttgcaa catctaatgc
    12181 ttgtttctct gattttgatt tatgccaatc aaatacattc caattcattt ttaatccaac
    12241 catataaaat gtttcaaagg aattgtttag catatttaaa cctggatttc cataacctgc
    12301 ttgaccaaaa gcatttagtt taggtaaatt actttttgac aaaatatttt ttgactgctc
    12361 aatttgttgg ttttgcaatt tgaaaagctc aagttctgga cgatttccgt tggtgttaat
    12421 tgtcgtaatg ggttgtacga attccacatc atttgaaata acggtacttg ttaaagctgc
    12481 taaattttga atttctttaa tgcgttgaaa atcattttcg gttaacgctt gttttatttt
    12541 tagaatttca gcttctaaga cttgttctga tgatggcagt aaagcaccaa attttaccgc
    12601 cgattgtact tcttttattt tttctaaaag caaattcctt ttggaaatca acaagttttt
    12661 ctgctcttgc aataacaaaa ccgacataaa atgataattt atttttgatt tcaatggata
    12721 caaattcacc gcaatttgtt gttgttgcac tttggtttgc atttgtttca attgcatatt
    12781 ggcatcaatt aaaccaccat tataaagcaa ttgattgaca tccaaagtcg ctcgatattg
    12841 atctttgtta gctggattta cggaaatatt tggtaaacta attggcaatt gtgtaacctc
    12901 agattgatac gttgcttgtg cgttgacatc tatttttggg agttttgctt tttgtaaagc
    12961 atcggtttca aatgtgattt tttggtctaa caattgcgat tgtttggcta aaggataatt
    13021 tttatctacc aagttgtaac attcttccaa agtcaacttt tgttgcgctt gaaggaaagt
    13081 tccaaaaaac aggaataaaa ttattattaa atttctcatt ttttaatact atttataagc
    13141 attgaagcta tgtgtttttt tcgttcgatt aacaagttgt tatatgctga ttcattaaca
    13201 tttgttattt tttgaagtaa tggtgcacca ataaatggaa aaatggctaa cgaaaacata
    13261 tcaatcaata attgttttgg atttacattt ttgattgttc cattttgaat gccttcttca
    13321 atttggatta aaaagcgtgt tgattttgga aattcagctt gactaaaaaa ttgattagca
    13381 aaatctggat tgttatttaa ttcttgaata atgaaagtgg gtagaaatct attttccatt
    13441 acaaacgaaa tgtacttgtc ggtaaaattt ataatctttt caaataacga atcttctgaa
    13501 ttcaatactt gatgaatttg tggtgccaat tgcatgaatg cttttttaaa aacggcttca
    13561 aaaagcaatt gtttgcttct aaaataataa tgaagcattg ctttgttgat tccagcttca
    13621 tctgcaattt cttgcattcg agcaccagct aaaccttttt tgtgaaagat tttgatagca
    13681 cttgcaaaaa ttaattcttc cgtatttgat tctaattttt tcattcaact aattagttta
    13741 accatttggt taacaaagat acataaaaaa agtagttcac aatgaaatac ttttttattt
    13801 tttctctttt acaaacatat taaacagcaa tccacccatt aatccaccat acaacgtact
    13861 gtttaatggt tttgaagtaa tggcacaagt tcctgacgaa caaccaacaa agtaataata
    13921 agcataacca gcaattaagc ctactactat tccaattcct gtaatgatgt accctttttt
    13981 tgacattaat taattttttc taaaataaca ttaattaatt cttcttttga taacactcca
    14041 gattgtcgcc ataattgttt gccattttga tacaaaatca tcgttggaac accacgaact
    14101 tgttgcattg atgctaaatc ttggttttta tcaacgtcta ttttgataat tgaaattttt
    14161 tcgcctaaac tatctttaac ttcttttaaa attggcgcaa gcattttaca tggtccgcac
    14221 caagtggcaa aaaaatcaat caaaactggt ttatcagaat taataatatt ttgaaaacta
    14281 ctattcattt ttagttgatt tataagaaac attacatccg tttggactac aacccaaatt
    14341 aaaaatagct tgaattaaaa agaatattcc aaatacaata aaaaaccatt catgagttat
    14401 gtaagcattg taaaaaagaa acaatgccaa cgcaattcta aaaaatcgca taaaatgcca
    14461 attggtaaag agtaactttt tcattatttt agtgtagatg gacaaacgta atttgttgtt
    14521 tttattcccg tatttttaat agccgaaaat cctccaagaa cattgattac attatgatag
    14581 ccacgtgctt ttaaaataga agctgcaatt acagaacgat aaccacctgc acaatgcaaa
    14641 taaaattctt cttcttttgg aaactcagct aaatgatcat ttataaaatc caatggtgtt
    14701 gaaggcacgt caactatatg ttcagcggta tattcacctg gttttctaac atcaaaaact
    14761 ttagcatttt cgtttatttt ttcttccaat ttttcagctg aaaccgactc aagtgtatca
    14821 tattctaatc cagcattttt ccacgcttca aaacttcctt cgagataacc aattgtgtta
    14881 tcataaccta ctcgagccaa tcgggttatg gtttcttctt ctcttccttc tggtgtaact
    14941 aaaagaattg gctgtttgta atctaaaatt aaggcaccaa cccacggagc aaatccacca
    15001 tcaattccaa taaaaataga ttttggaatg tgtccttttt caaaatcatc ctgatgacga
    15061 acatcaagaa tcaaagcatc ggtttcattg gcaaccaatt caaaagtttt ggcatcaaat
    15121 gctttcgctt cactaattat ggtttctaca ttttcataac cttctttatt taacttgaca
    15181 ttcattggaa aataagctgg cggaggcaat aaaccatcgg taacttcttt gacaaattct
    15241 tctttggtca tatttgctct taaagcataa ttagtagctt tttgttcacc aattgtacca
    15301 accgtttctt tgcttaaatt ttttccacaa gcacttccgg caccatgagc tggatataca
    15361 atcacatcat cggcaagagt cattactttt gttcgcaaac tatcaaataa caatcctgct
    15421 aattgttctt gtgtcatgtg agccgctttt tgggccaaat ctggtcgacc tacatcacct
    15481 aagaataagg tatcaccgct aaacaatgca taatctttac cgtttttatc tttcaataaa
    15541 taacaagaac tttccatcgt gtgtcctggt gtatggagta atgtaatggt aatatcaccc
    15601 aattgaaaca cttcaccgtc tttagcaata tgagctttga aactcggatt tgcatttggt
    15661 ccaaagacga taggcgcacc cgttttttcg gcaagagtaa catgtcccga cacaaaatca
    15721 gcgtgaaaat gggtttcaaa aatgtatttt atttttgcac cagcagcagt ggctttgctt
    15781 acataaggtt gtacttcacg caaaggatca atgatagcaa cttctccatt actttcaata
    15841 taataagcac cttgtgctaa acatccggta tatatttgtt cgattttcat gttttaattt
    15901 ttaatagtat tacaaattta agttttataa tttttttgaa aggtaaccaa tgttactttt
    15961 aagatttatt ttagaaatag ttctttgata atgatataaa aacccattgt caatacaaaa
    16021 tagccaaaac caatttttaa ttttgtagct gttatttttt ttgaaatcaa atttccaata
    16081 ataacgccga caatagaaca agctgtaaaa gttaaaagca ttttccaatc tatttctaca
    16141 tttgaactta aatcgcctag aaaaccaatt agtgattgaa tggcaacaat aaacaatgag
    16201 gttccaatag cttttttcat cggagttttt gccaacagta ataaagcagg aataattaaa
    16261 aatccaccac cagcaccaac aaacccggct actaatccga tggatattcc ttgaaagaat
    16321 atagaaccat aatttagttt tccatcaaga attaattcac tttcttttaa tggttttatc
    16381 attcgaattg ccgccaaaat cataacaact gcaaaaacca acatgattaa aaccgatttg
    16441 tgtaatgtaa ataaatcggt gtcaaaaact acatcgggaa tggaaggcat tatgtatttt
    16501 cgggttatga aaacagcaat aaccgttgga attccaaata aaaatacttt tttaaagtca
    16561 actaattttt gattcgcttt ctggattcct ccaaacaaag ctgttgaacc aactgcaaac
    16621 aatgaatagg ctgttgccaa aacaggttca acactcatga tataggtgaa aattggaacc
    16681 gataatatgg atccgccgct tccaataatt cctaatgaaa tgccaactaa aactgcaaat
    16741 atatttccaa taatcaaaga agtttccatt acaattaatt taatacaaag gtttggcttt
    16801 ttgtttttaa ccaaagcaac atttgttact aagatttcac tatttcaata atagcgcgat
    16861 gtagttttat ttgtccttga ttttctaatg cttttaataa tctagaaatt acaactctag
    16921 atgtatgcaa atcataagcg atttcttgat gcgtgctttt aatttctttt gatgaattga
    16981 tttttgattt ctccaataaa taattcatta aacgatcatc catattcaag aaggctaaac
    17041 tatcaattgc ggctaacatt tcttttaatc gattgttata gctgttaaaa acaaagtttc
    17101 gccatgactt atatttaccc aaccattctt ccatgaagtt tacaggaatc atgactactt
    17161 ttcctttgtt ttcggcaatg gctctaattt cactttttgt ttcgcccaaa caacacgcca
    17221 tcgtcattgc acatgtttct cctttttcga tgaaataaag cagtaattca ccttcgtcaa
    17281 aatcttctct tagaatttta atagcacccg aaattaataa tggtattttt ttgatataat
    17341 ctccaaaatc aatcaaaaca tcaccttctt taaaatcttt taaagtggct acctgagcaa
    17401 tttcatctat tagtttatct tcaaaaacaa agccgtagtt ttcttttagg atagtatgca
    17461 tactttaaat ttatttcaaa tttggtaatg aatacccaaa aataaaggta atgtttaaat
    17521 aattgattaa aaaagttaaa agttttatac aatttttaat aaaaacaacg tttatataat
    17581 agtaacttgc acactaaatg acaacaaata aattatggag ccaataaaaa ttctttgggt
    17641 tgatgatgag atagatttac taaaacctca tatcttattt ttagaaaaga aaaattataa
    17701 cgtaactacc tgtaataatg gacgagatgc catagatgtt tttgaagaag gaaactttga
    17761 catcgttttt cttgatgaaa acatgcctgg aatgagtgga ctggaaacct tatcagaaat
    17821 gaaagagaaa aaatcttcaa caccagtgat tatgattacg aagagtgagg aagaatatat
    17881 catggaagaa gctattggtt caaaaattgc cgattatttg ataaaaccgg ttaatccaaa
    17941 tcaaatattg ttgagtttga agaaaaacct agaccattct cgattggttt ccgaaaaaac
    18001 aacgcttgat tatcaaaaag agtttcgaaa aatagctatg gatatggcta tggtaaattc
    18061 ttatgaagat tggatagaac tttacaaaaa actaatgttt tgggaattgg aacttgaaaa
    18121 catcaacgat caagcgatgt tcgaaatttt agaaagtcaa aaagtagaag ccaacgttca
    18181 gtttggaaaa tttattgagc gtaattatga agattggttt gaaccaaaag cagataaacc
    18241 agtgcaatcg cacaatttgt ttagagaatt agtcgtgcca gaattggtta aaaaagaaaa
    18301 accagtgtta tttgtcgtaa ttgataattt gcgttatgac caatggaaag tgatggaaaa
    18361 cgtagtaagt aacttttata aacttgaaaa agaagtgcct tattacgcca ttttgccaac
    18421 agcaactcaa tatgctcgaa atgctatttt ctctggaatg ttgccaagtg aaatggaaaa
    18481 acaatttcct caatattgga aaaatgatgt cgaagatggt gggaaaaacc tttatgaagc
    18541 cgaattttta tcggcacatc taaaacgttt gggtttgaat attaaagaag attattttaa
    18601 aatcaccaat ctgcaaagtg gaaaaaaatt ggccgaaaac ttcaaaggtc taaaagataa
    18661 caatcttgta acgatagttt ataattttgt tgatatgctt tcgcatgcca aaaccgaaat
    18721 ggatgtagtg aaagaactgg cttctgacga taaagcctat cgctcattaa cattgagttg
    18781 gtttaaaaac tcaccgttgt tggaaattat tcaacaagcc caaaaattgg ggttcaaatt
    18841 aattattact actgatcacg gaacaattaa tgtgaaaaac ccaagtaaag taattggtga
    18901 taaaaacaca agtttaaatc ttcgttacaa aacaggcaga agtttgacct atgaagataa
    18961 agaggtttat gccgtgaaag atccaaaaaa aataggatta ccgactataa atatgagtag
    19021 ttcttatatt tttgcaaaaa atgatttctt cttagcttac gtcaataatt acaatcatta
    19081 tgtgagttat tatcgaaaca cctaccaaca tggtggtatt tctttggaag aaatgattgt
    19141 accgttttta gtttttaatc ctaaataaag taattagtga atagtaatta accaacaaat
    19201 agaatactat tcacttttca ctaattacta attgcttaaa atatggaaat tcaattttca
    19261 attgaggaaa ttcagcaagt agctcggaat attttatctg aaaatccaaa aaaagtaatc
    19321 ctctttcatg gcgaaatggg tgcaggtaaa acaacactta ttaaaacgct ggctagagaa
    19381 cttggtgtaa ctggagctac aagtagtcca acattttctt tagttaacga atataaaatt
    19441 acgtcaaatg aattcgtata tcattttgat gtctatcgat tgaaaaatga aagcgaagca
    19501 ctcgattttg gcatcgatga ctatttgtat tcaggaaatt ggtgttttat cgaatgggct
    19561 gataaaattc caaatctttt gcccgatgcg tattcggaag tttttatttc gatacttccc
    19621 gatggaaaaa gaaaagtagt attgcaataa gtaatttatt ttttttgtaa cttcggctaa
    19681 atttacctaa ccacgatggc tactacacca tttacaaagc aacaattgct tccacaagaa
    19741 gaaatgttag aaataattcg tcataaaagc gaattgttta ttggtattcc caaagaaact
    19801 tcctatcaag aacgaagaat ttgcctaacc ccagatgccg tgaactcact ttgctcacat
    19861 ggtcataaaa ttatgattga agcaggtgca ggattgtctt cgagctattc agacaaagac
    19921 tatagcgatg ctggtgcaac aataacaaat gataccaaaa aagtattcaa ttgcccaatc
    19981 atccttaaag tagagccgat gtctatggaa gaaattggat tgctaaatat gaatgccatc
    20041 gtaatttctg ccattcaatt aaagactaga aaaaaagaat atttcgaagc cttatccaaa
    20101 aagaaggtta ctgctttagc tttcgaatac atcaaagatg aagacggttc atatcctgca
    20161 gtcaaatctt tgagcgaaat tgcaggaacg gcttcaatac taatcgctgc cgaattgatg
    20221 attaacaatg agtatggaaa tggcttgctt ctcggaaata ttacaggcgt tccaccaacg
    20281 gatgttttaa ttttaggagc aggaacagta ggcgaatttg ccgcaagaac cgctttagga
    20341 ttaggcgcaa cagtaaaagt gtttgacaat tcgataacca aattacggcg tttacaaaac
    20401 aatttaaacc aacgtgtgtt tacctctact atacaaccca aaaccttgtt aaaagccttg
    20461 agaagaaccg atgtcgtcat tggtgccatg cgtggtaaag accgctgtcc tgttgtagta
    20521 accgaaacga tggtagagca catgaaaaag ggtgcagtaa tagtagatgt tagtattgat
    20581 actggtgggt gctttgaaac ctccgaagta accacacacg aacatccaac gtttattaaa
    20641 agcaatgtca tacattattg tgttcccaac attccttcac gatattcaaa aacggcatcg
    20701 ctatctatta gtaatattat tacaccttat ttgctacaaa tagccgaaga tggaggatta
    20761 gagcatgcaa tcagatgcag taaaggtttg aaaaatggtg tctatttata tcacggaatt
    20821 ttgaccaaca aagccattgg agattggttc aatttgcccg ataatgatat taatttaatt
    20881 gttttttaaa ataaaagttc tcgatacaat ttttgtttca ctttgttaca caaaaaccaa
    20941 tcgaacagaa gcatcggaac aaaaatcaat atttaatctt cgtcacttcg atttataaaa
    21001 gtggaaacgc tagttttaag ttttataaat tgtatcgaga agtaaatcct aaaataactt
    21061 ctataaacta tctgtcaaag caaaacaaat gattattttt gcaaaaaaaa tactcaacag
    21121 atgaagtttt accaacgttt tgcctactac ttagtgggtt ttgttatcgg aatgtttttt
    21181 gtggcgatgg tttggagcgg aaaagacaca cgctgcaatt attttccaaa tgctagagta
    21241 ttgaatgacc ttagaaacaa accttttcat tatgataccg aagcttctcg caaacttagt
    21301 gaggattggg tggatagtat agacgttaaa aatactttgc aatatggcga tgttgacttt
    21361 gataaaagca atattccaga cgaaggagga aaacgctaca ttgttgaagg taaaaccacc
    21421 aaggatgttg aaattactct agaaatcatt aactacgaga gcaaagcagt attaaagaat
    21481 ataattagga agtaataact ctagttaagt aaaaaaaccg ttttgaaagt tcaaaacggt
    21541 ttttttagta cttaattttt tatcttttat tagccaaaat atcttctggt ttaacagcaa
    21601 tagaaactcc tggcaatttg attggagcac ccatttgtgc taagaaactt ccttgaactt
    21661 cacctttttt ataagtagtg aatccaattc tgtataaacc cattactaat gattttgaag
    21721 ggttagaagt atcacttgta attctcatta atgagttgta tttttttcct gatggttgtg
    21781 ccggcatttc agaagaatca tcattagatt caattgttgt ccaacttgct gattttgctt
    21841 ttcctgcaac atcttcaatt ttcatgattc cttctgctcg ttctaaggta acccaagtat
    21901 cgaaataatt gctcgtgtca gcagcatttt cagtataatc tgcagatgca taatctttat
    21961 ctgttggttt tacactatct ggtgctggtg aaatcattct aattccgatt tctggtgctg
    22021 caatgggtat ccaaacgtag atgtagtaca ttttctttcc gttttttact tcgtcgggtg
    22081 cagcacctgg tttgatataa ccaaaataac ttgtcatatc agtataagga acgcgaattt
    22141 cttttcccat cattgatttt ttggctaaat ctgctccgaa tttgtcaagt ttttgcgccg
    22201 ttgcggttaa tcccattgct atacaaatag cacttgaaag aataattttt ttcataattt
    22261 gttttgttag tttgcctact cttaaggatt ttcggttgct ccatttttat gttaaatttt
    22321 agaataataa aaataataaa aatatcagaa caaatgaact catgctgata aatagcagtg
    22381 atttactttt ctcttttgag aataaaactg ctggaaaagt aaaaaggatt aaagcgatta
    22441 aagtaattaa atttggggtg cctttataga gtggttttaa aaagaaagga agtgtttcaa
    22501 tgatatcagg gctaaaacct tttttatgta aatatagtaa gtcatcaaat gatgcaataa
    22561 agcaccagtg aagtattatt aacactgcaa agaaatatat cgatataata atttgattct
    22621 tttttttcat tttttattgt tgagaataag aaataaatct tatctctagt tttagtaaaa
    22681 atagaataaa gattgacata attcaaaaag atgcccagaa aatgaaaatt cgttaggttt
    22741 caattattat acgttgacaa ctggtgcata tctgtaaata aaaaaaccgt cttgaagcaa
    22801 atcaagacgg ttgagaatat atagttttag tgaataaatt ttaactattt agcattactg
    22861 gcatcactaa cattgttact gtttcacctt catccaaacc atcaattggc gttagaattc
    22921 ctgcacggtt tggcatcgac atttccaatt gaatttcgtc tgaggttaag ttggtcaaca
    22981 tttctgaaag gaaacgtgag ttgaaaccaa tttgaatatc gtcgccttgg taatcacatg
    23041 tcaaacgttc ttctgctttg tttgaataat caatatcttc tgcagaaata ttcaattcgg
    23101 caccagcaat ttttaaacga atttggtgcg tagttttgtt tgaaaagatt gctacacgac
    23161 gcacagaact taaaaacaag tttctgttaa tcattaattt atttggattt tcttttggaa
    23221 taacggcttc atagtttgga tatttcccat caattaagcg acacatcaat acataattat
    23281 caaaagaaaa agtagcattc gaatcgttgt aatcaatttt tacttcggta tctgttgctc
    23341 caagaatgct ttttagaatg ttcaaaggtt tttttggcat gataaaatca gctacctcag
    23401 aagcttttac atccgttctt gcatatttca ccaatttgtg agcatcagta gctacaaata
    23461 ttaatccttc gggagaaaat tgaaaaaata caccgctcat tactggacgt aaatcatcgt
    23521 ttccggcagc aaatattgtt ttgctaatag ctgttgccaa aacatcggca ggaacaagtg
    23581 ttgacgatgg ttcttcgatt acttgtgctt ttggaaattc agttcctgga gcataagcaa
    23641 tagcatattt tccagagttt gaactaattt caatcgtact gttttcttca acagtaaacg
    23701 ttaagggttg ttctgggaat gtttttaaaa tatctaataa aagtttcgca ggaacggcaa
    23761 cactaccttc gctactagaa tcgatttcta aagtagcaga cattgtggtt tctaaatcgg
    23821 aagccgaaac ggttagtgtg ttattatcta attcaaatag aaaattatca agaattggta
    23881 acgtattgct actattgata acacttccaa gcacttgaag ttgttttaat aagtatgaac
    23941 tcgaaactat aaatttcata tgtaagtatt tattttttta gctgttatat ggtactgctt
    24001 ttctttaact gtgtttgctt acgcaaactc atttttaagt ccgatttcat ataactttaa
    24061 tttttacaaa tatatcttaa actaatagat tttgaaaagt tttttattaa cattacttgc
    24121 tgtattttct cttgcgtaaa atggtaaata aaacaccaaa aagaagcagt acaactagcg
    24181 gtaagccaac ggttatgatt tgtgatgtgg tatattgggc atatactttt tctttatcga
    24241 gcattggtaa atctacttct ttgcttctaa tgttgataag tccgttgtcg tctaggaggt
    24301 agttaacaca attgagcata aattctttgt tggcatacaa ggtgtttgtc catttgtcaa
    24361 aaccaagttc gagcggttgg tagtttttgt cgagttggtt tttgatgaca ttaccatccg
    24421 aaatgacaat cattttgctg tcttttcctg aatttgaaaa actagcttct ttaaacgcca
    24481 agactctatt ttcaaatacg gaatgaaact ttccttctag caaaacggct acaggataat
    24541 taccattgcc ttcaaattcc ttttgttctg gtcgttcttc taccatattc agatttacct
    24601 cggttggtgt tccaactaat tttgaatatt gggaagaagt caataatact gtttttttaa
    24661 tatcattttt gagtagttca attgggcttg caaattcaaa ttttatgccg tccaagttac
    24721 taacaatcgg attttttgag tttgggtaaa tcattggaga aaacatccat ggaaattggg
    24781 tgtattgtgt ggcacttcct ttatcgccag tagccaaaga aataggtgtt gccataacgt
    24841 ctttaatcaa atcgggttgg attcgtattc cgtatttgaa aaacatatcg ttaaggttta
    24901 aatctctagg aaaggcaaga ttttcacctg tttgcatcaa actgtccatt tccatgttta
    24961 cctgatccat taaccaaatg gtttttccac cgttgatgac gtattggtct atgacttgtt
    25021 tttcagcatc ggtaaaaact tcggttggtt tggcaataac gactaaatca tattttttta
    25081 aatactttaa agtttctgtt ggttgttttg ctacagaatc taaagtgaat ggtccgatgt
    25141 aatagttctc tttaacactt cgagcaaaat cggcgatgta tttatcttct agttcaccat
    25201 ttccatttag gatggctatc ttcttttgtt tttctttgga aacggtgttg aaagcattga
    25261 caaaagcata ttctagatgt tgcacagagc ttactacttt ctcggcagtt gaagcgccaa
    25321 tcatgttttt taacaacggt actttcacgc ttttgtcgcc ataagtagct attgcccaag
    25381 gaaaaactac ttcttgtgtt tgttgtcctt tatcgtttag agtaacgtta acgggtgtta
    25441 atccacgttc cataaacgat tgaaatagga catctttatc cacttcttcg tcttccatcg
    25501 gattgacaaa ttgaaaaatg atgttaggat ttttagattt gaattcttct aataattgtt
    25561 gggtttccgt ttgtagtttt ttaaattctc ctgggaaatt tccttctaag aaaacatcaa
    25621 catacaacgg ttgggtaact tcatcaataa tcttcaacga tgtttccgaa agtgtatagc
    25681 gtttatccgc agttaagtca aatcgtttga aaacaaaatg gctggctatg tttattccaa
    25741 caacaatgag taggacagaa gctgtttttt ttatcaattg tactttcatc agattaagat
    25801 tttaaagctt ttagtttata aaccgtcatt gttaaaaata aaaaagaaat actcaaaaaa
    25861 taaatcacat ctcgtgtatc aataacacca cgcgccatgc ttttaaaatg ataatccatt
    25921 ccaatgcgag caatttcatc ttcaaaacct tttacttgat tcgcaattcc ttcaaatcca
    25981 taatagaata aaaaacacaa gaataccgat agtataaacg atacaatttg attttcggaa
    26041 agtgtagaag taaagatgcc aatagaagta taggctgcta ctaaaaataa taatccaaaa
    26101 tatgaaccca tgatgcttcc taggtcgata tttccggctg gtaaacctaa agatgaaatt
    26161 acgtaaacgt agataaacgt tggaataatt gcaaaaatga tgagtaaaaa ggcaccaaga
    26221 aactttccgt ttacaatctg ccaaaccgac aaaggttttg ttagtaataa ctcaatggtt
    26281 ccttgtttga tttcatccga aaaacttctc atagtgactg ctggaattaa aaaaatcaat
    26341 atccaaggcg aaagtgtgaa gaaaggtgtt aagtcggcaa aaccactttg taatatattg
    26401 tattcgccat caaaaaccca taggaataat ccgttgagta aaagaaaaat ggcgattacc
    26461 aaataaccta tgggtgaacc aaaaaaggat tttatttctc gtaatgcgat tgatttcatt
    26521 tatatatttt ttgtttaaaa gtttaaattt gttcaaaaag gaaacaaaat ctaaccaaat
    26581 catacatttt aaactttaat taaactattt taaagttgct attttatcca cactccaagc
    26641 ttctggttta tcgttgaata gtttcttggt aaatgtccaa acttcatcga ataaagccga
    26701 ttgtcggtag ttttccaaat cttgttcggt ttcccaaaaa ctatatgtga aaaaaataca
    26761 cggattgttt ttgtcctgat ataattctag ataacgattt ccttcggagt ttcgtatttt
    26821 ttctttcatt aaatcgaagt ttttcaagaa ctttgaaata ttttcctgat gaaaactcat
    26881 ttttactatg cgtatgaaca ttttttagct tttcagatta tagagttaat aaaaatcaat
    26941 agttacagtg tcacgaaaac ttaaacctag caaagacgaa gctgagccag aatttttcgg
    27001 attactttta taaagggcga tttctaggaa atcagcatca ttaaatatcg ctaatttttc
    27061 gctttcaaac tcttttaaat ttcggggttt gtttttccca atatcagaat atttagcgag
    27121 aatggtttta attactttgt ttttaaaacg cacttcataa tttcgacctt tgctttgttc
    27181 caaaaacatt tttttggtaa tgttagttac agcattacca aaatggtcaa tatatatcac
    27241 aaaacctttc aattgatttt cagaaacaat aagttttaaa tcagtagctt cctttaatga
    27301 ttttatttct tttccaataa cgtttaataa tccacctttt gccaaatgaa cagcaaccat
    27361 tataaaaacg tccaattcag acgcatccgt gtgcaaacgg tcgtgaatat tgatagcaac
    27421 tattttttga ggtactattt tttcaatcaa catgctcaaa ataccattgt cagcacaaat
    27481 aaagtaatgg tcattccatt gcatggcaat atgttgggta gtttctttgc taatttcgat
    27541 gtcaacacca attaaatgca ccgttccttt tggaaaacta gagtaggctg caccgataat
    27601 gtagctggtt tcagaaatat taaacaaatc aatatcgtgt gaaatatcta caatttgtgc
    27661 ttcgggaaac tcggttagaa ttttcccttt caaagcacca acaaagtgat ccttcaatcc
    27721 gtaatctgtt gttaaagtaa ttattgacat tgattgttta taacttcatt gaaattgatt
    27781 ccaaaaatag aaaaacctaa tcttttattt gattaaattt gaatgcaaag ctaatttatt
    27841 tttagcaaaa aaattaattt aatacttacg cttttgaacg aacgcatcat cgaattacac
    27901 gacatcgctc caaaagaatt ttggggtgct caagatacac atcttgaaac gatcaaaaaa
    27961 tattatccaa aactaaagat tgtagctcga ggtacaactt taaaggcttt tggtgaaaaa
    28021 gaagagctcg acgagtttga aaagcggttc aaccgactaa tgcttcattt tacgcgctac
    28081 aataatatag ataccaatgt tatagacaga gtaattcaaa gtgattctaa tgaagagcaa
    28141 cgaatgccaa acagtgataa gattttggtg catggaatag gaggaaagct aattaaagca
    28201 ttaacgccaa atcagcaaaa attggtagaa tatgttgaaa aaaacgatat ggtttttgca
    28261 attggaccag caggtacagg aaaaacctat actggtgttg cgctagcagt aaaagcttta
    28321 aaagaaaaac aagtaaaacg tattatacta acacgtcctg cggttgaagc tggtgaaaat
    28381 ctaggttttc ttccgggtga tatgaaagaa aaactcgatc cgtatatgca acctttatat
    28441 gatgcattgc gcgatatgtt gcctccacaa acgctggaag attacctctt aaaaggaatt
    28501 attcaaatag caccattagc tttcatgcgt ggtcgaacat tggataatgc ttttgtaatt
    28561 ttagacgaag ctcaaaatac tacgcacaat caaatgaaaa tgtttttgac acgtatgggt
    28621 aaaaatgcta agtttatcat tactggcgat cctggtcaag tagatttacc aagaagaaca
    28681 atatctggtt taaaagaagc cattttagta ttgaaagata ttgaaggtat tgggataatt
    28741 tatttggatg ataaagacat tgttcgccac agattagtca aaaaagttat cgatgcttat
    28801 aaaagtatag agaatcatga ttaaatagat gtttttgaaa tacatttaat aaaaagccaa
    28861 ttgcttctgt aattggtttt tttgttgatt ttcagcaaag tagatttgta aatggtttaa
    28921 ttctataaat ttctgtaaaa atcttgtaaa tacttatgta ttggttttat tgaaatttgt
    28981 aacataaaca tataaaaaca aacattatga aaaagctatt tttatttatc ggattggtta
    29041 gtattaactt catttttgca caacaaaacg aaattgatat tactccaaat aattcttggc
    29101 ttaaagcggg tataaccgcc gaataccagt tggagatgct agcaattttt ctacatttaa
    29161 tttgggttta gatttaagag ggcaatattt atttaaccct aactttggaa ttggattagc
    29221 ttcgggttat acacattatt ttgctaaaaa cgattttgat gattttggaa taattcctgt
    29281 tgcaggtttt gcacgatatt attttactaa aagcggcata tttatcggta cagatttagg
    29341 gtatgggttt ttatcaaatg ttgataataa tgaaggagga atgtatctaa atccacatct
    29401 tggttatcat aatagagatt ggaatattta tgcattttat caaaacacta ttgctcaaaa
    29461 taatactaat ttacaatctg ttggagttgg agtaacttat aatataagat ttaagtagtc
    29521 aattttattt ttgatgatga ttaaaaggag aaagtttatt tttccttttt ttatttttga
    29581 tattgtttaa caatggtaaa caaagtactt ttgtaaaaca acaacacaac attatataat
    29641 gaatacaatc actactacag attttgattt cccaaaccaa aaatcagttt atagaggcaa
    29701 agttcgcgaa gtctataaca tcaatgacga gttattggtc atgattgcta ctgatagact
    29761 ttcagctttt gatgtcgttt tgccaaaagg aattccatac aaaggacaaa ttttaaacca
    29821 aattgccact caatttatgc aattgacgga agatattgta cccaactggt tgattgcaac
    29881 acctgaccca aatgttacta ttggacattt gtgtacgcct tttaaagtag aaatggtaat
    29941 cagaggttat ctttctggac atgcagccag agaatatgct gcggggaaaa gaacgatttg
    30001 cggtgtagct atgcctgaag gattaaaaga aaacgataag tttcccgaac cgattattac
    30061 gccaactacc aaagccgaaa atggttcaca tgatgaagac atttcaagag aatcaatttt
    30121 agaaaatggt attgtttcta aagaagatta tattgtttta gaacaatata caagagcttt
    30181 atatcaacga ggaacagaaa tcgcttataa tagaggtttg attttggtag atacaaaata
    30241 tgaatttgga aaaacaaaag agggaaaaat tgttttaatt gatgaaattc atacacctga
    30301 ttcttcaaga tatttttatg ctgaagacta tcaatcacgc caaaataatg gtgaacaaca
    30361 aaaacaatta tctaaagaat ttgttaggca atggttaatt gctaatggtt ttcaaggaaa
    30421 ggaaggacaa caaattcctg atatgagcga ggagtatatc gaaaatgttt ctgatcgcta
    30481 catcgaattg tatgaaaaca tattgggcga accgtttgaa aaagccaata ttgaaagcat
    30541 tcatgaacgc attagaatga atgtaatgaa ttatttaaat agttaaatga cttatttata
    30601 atgaaataat gacaaccacg gcactgccgt ggttttttta tggtgtaaat taaagtttct
    30661 ttctttttgt gttacaaatc aaaaagtgct tcgtctaatt gatatattgg ataatcttaa
    30721 tcaaattgtt caaatgttaa attttagtta ataaaatagt actatgtaaa accaaatacc
    30781 tttttatata tctttgtaga agaattaatc atcaatcatc aaaatcaatc atcatgaaaa
    30841 caatcatttt aatcggaatg gtttctttag gatttctaaa tcaaaaccat gcagcaagcg
    30901 cagaacaaac aagtttcatc aatcaatcag acaacgaaca gtttttagaa ttagtttcgc
    30961 ttaaaacaag aattaaagtt gtcgaaaaca ctaccgaaaa gaatttctat tttgacccaa
    31021 caacggtttt gccaaaagct atcaaatctt tggagcaaga aattagagaa gacaatttaa
    31081 ttattgaagg taatatagaa acccaagtaa cattgtctgg attatactct acgtctatcc
    31141 aaaaaattat tcaagaagac attcaagtta cagaagcaaa agatttggat gtttatcaac
    31201 ctttagattt tgaagctata aacaataacc ctaatggtgt tattgaactt caaaacaaat
    31261 gttctttgaa acaagagaac attaaacttt aaattttatc gttgtaaaat ttggttttag
    31321 ttgtgaaaag caccatttcg gtggtgcttt ttataaaact ttactaaaat tttatattcc
    31381 aatcaaaaat aactaagagt ttttactctt ttctatatat acaattatta atcaaaaatt
    31441 taatcatcat ggtaaaaaca atcattcatt taagtttagc gctagtcgct tttgcaaatg
    31501 tttcatttgc gtcgcaacaa gtaacttcag aaactcgtac cgagttaatg tatagttcaa
    31561 acacaccact ttgtcttgca attgtgaaag gcgacgtgga gacagtcaaa aaatttatcg
    31621 aatatggcgc aaatgtcaac gaaacctcta atggattaac accattaatg atagctgctc
    31681 gttacaataa agttgaaatt ctaaaattat tggtggacaa tggtgcacat gttaacgcaa
    31741 aagatgaaaa aggatatacg gcattaaaat atgcaacgtt atcaaacgct acagccgcta
    31801 tcgcttatct aaaagacgtt tagttgacct attttctttc atacattata atagggttaa
    31861 aagaaaaagc atcacaattg tgatgctttt ttgattattt tttaaaataa gagaaattat
    31921 ggttgttctc aattttcaac aaactttcgt aaattaattt tatcacattt tcaacatcat
    31981 ctttgtgcac catttccacg gttgtgtgca tgtaacgcaa aggtaacgat attaaagctg
    32041 aagccacgcc accattgcta tatgcaaaag catccgtgtc ggttccagtg actcttgaag
    32101 ttgcgtgtct ttgaaaaggt attttctttt cttctgccgt tgaaacgatt aattctctta
    32161 acacgttttg tgtggctggc gcataagcaa taactggacc tttacccatt ttcaaatcgc
    32221 cttcaatctt cttgtcaatc attggtgttg tagtgtcgtg acaaacatca gtaacaatag
    32281 caacatttgg cttaatggtt tgtgtaatca tttcggcacc acgaagacca atttcttctt
    32341 gaaccgaatt cacaatatac aaaccaaaag gaagtttctt tttgttttct ttcaacaaac
    32401 gagctacttc ggcaatcata aatccgccca ttctattgtc aatcgcacga caaacaaatt
    32461 tatctttgtt taaaatcata aactcatccg gataggtgat aacacaacct acatgaacgc
    32521 ctagtgtttc cacttcttct tttgtagagc aaccacaatc gataaaaata ttgtgcaacg
    32581 atgcgttttg ctctttatcg cgatttctag tatgaatggc tggccatcca aaaacgcctt
    32641 ttacaattcc ttttttagta tgaatattga ctctttttga cggtgcaatt tgatgatccg
    32701 aaccaccatt tctaatcacg tagattaaac catcgtctgt aatataattt acataccaag
    32761 atatttcatc gctatgacct tcgatgacaa ctttaaaagc tgctttaggg tttattactc
    32821 caaccgcagt tccataagta tcggtgataa agtcatccac ataaggcttt aggtattcca
    32881 tccatatttt ttgaccttct gattcatagc ctgttggcga tgcattgttg aggtattttt
    32941 ctaagaaagt ggtagatgat ttggtaagta tcgatttagt tgccatattt tttttttgct
    33001 aaaatataaa tttggcatta gaattgttaa tataatattt atttttacac taaatatttc
    33061 tacatgctaa ctaaaaagtt gcttttatta ttgttgttct tttgctcttt tacttttggg
    33121 caaccaaaga aagattcgat tcgttctgaa gcaccacaga ttgaagttga tacaactttt
    33181 accgacacca ttcagctcga tgaagtatta atcagcaaaa atttatacga atacatcaat
    33241 aataaagatt ttgtgttgct tcaaaataga gtttataaag tctatcctta tgcaaaaata
    33301 gcttcggaac gattggttaa tttaaataaa tatatcgaca gattgaaaac tgacagagag
    33361 aagaaaaagt acctgaagtt ggttgaaaat tacatggaga atgaattcac agagcaacta
    33421 aaaaagcttt cacgaaaaca aggacagata ttggttaaat tgattcatcg tcaaactggg
    33481 caaaccactt ttggattaat taaagaatat aaaagcggat ggaaggcttt ttggtccaac
    33541 aatactgcaa aattattcga cattaattta aaggcagaat acaaacccta tcaagttaat
    33601 gaagattacc taatcgaata tattttagac agagcctttg caataggcag attacgcgaa
    33661 caaaaacccg cttttgacat tgacatggat gctttgacaa agtattggga aaacggcaag
    33721 aaaaagaaat agttttttaa gtaaaaatat accactattt ttttatacgc tacttatttt
    33781 catttcattt ccacatacat ttcagtgcgc aacggattga ttgatttagc atctttaaaa
    33841 tcgcgagtac taatagcagc cgattgtgtt ctcattcttc cactggcggt aatatcgttt
    33901 atcgcaatgt ttagtagtgg ttcgttgggg tcgccaagaa caccaagatt gccaatgttt
    33961 tctattacat taaagggtgc atttgcagca atgccagcag tataatcacc aaaaccattg
    34021 ttattaattg tttttaaaac aatgggttgc atggcatatt tgtgagaagg attgacattc
    34081 gattttgtaa aagaaggcga atcatacaat gtaaccgacc caacattttt gcccgtggtt
    34141 acatcgccaa tttgcactac attaatataa ggttttaaac aattgatgac caattcgctt
    34201 gccgaagctg tgccttttgt agtaagaata taaactttgt ttaaattcaa actgtttaat
    34261 ccgccaacaa atttattcag caaagaatta ggatcttggc tttctcgaaa agcttgctgt
    34321 ttttcattcc attgttgctt ggcaaacact tgattagtga attgaccagt aatcatgctc
    34381 gccaatttgg ttgctgtagc aatagaacca cctgaattgt atcgtaaatc gagtaccaaa
    34441 tcagtaacat tttgcgattg aaaaccacca aaaaccgtgt taagttggtc gtcataagaa
    34501 gcatagaatc cgttatacat cagataacca atattgtgtg tgccttgggt tataacagtg
    34561 tttataagaa ttgggttttc ggccagattg gttttggtta acgaaacaga attgccattt
    34621 ggcgtatatg caccaccatt ataatcggca agattgatgg tataggtatc acttgccaaa
    34681 agcgttcgaa agttgtcgcc cgttatagga attccatcaa ttgcataaaa caagtcgcca
    34741 cgatgaatgt tcttagtcgc cgcatccgaa tttggaataa tgtagcgcac ccagccaaca
    34801 acagaagtat ttgaagcatc tgtatatttt aacccataat ccatgccatt gtttttagtg
    34861 tttccagata atacgccttc aagtaaggag taatcgctat agataacgct aaatctatca
    34921 atagcattgt caattctaag atgattgaac aagtttgttg gagtttcaaa cgaatctaaa
    34981 aacgaattgt attcgccttg cgaactaaag cgattgtctg ccaaatcagg actttcgcct
    35041 tgccacaaat aatagtaatt catgccttgc caaacgaaat cgttgatggc tagtttggaa
    35101 ggaacagggt tatcatcaat gtcttcacaa ctaacgattg agataaatag aaatgtaaat
    35161 aagaggtaca aaagactttt tttcatagca gtttttcttt taataaacgt aaatttacta
    35221 agtttagatt aaaaacatat tatttgtaac aaaataataa ctgtatcgtc ttgattatag
    35281 aaccaaagtt aaaccaatga accaaaatga gtttatgcaa atcgtttctc cttttaagga
    35341 caaactcttt cggatggcta agcgattgct cgttagcacc gaagaagccg aagatgctac
    35401 tcaagaagtg ttagtaaaac tttggaataa gaatgaagct ttagatgagt ataatagtgt
    35461 agaagcgatg gcgatgacca tgaccaagaa ttattgttta gaccaattga aatcaaaacg
    35521 cgctaataat ctaaagattg tgcataataa ctacaccgac cgagaagcga gtttgcagca
    35581 aaaagtggaa gatagtgata cttggcgttg ggtggaaaag ataatgcatg agctgcccga
    35641 acaacaaaag ttaattattc aaatgcgaga cattgaagaa atggagtttg aagaaattgg
    35701 cactgttttg gagatgaatg aaacagcaat aagagttgcg ctatcaagag cccgaaaaac
    35761 gattagagaa aaaatgacaa aaacacacaa ctatggaatt ggatagaata gaaatactac
    35821 tagaaaaata ctttgaaggc gaaactagta ttgccgaaga aaaagaattg aaaaattatt
    35881 ttgcttcttc caatgttgcg cagcatttgg aacaatacaa gtcagttttt gggtttttct
    35941 ctcaagcaaa acaagagcag tttacagcca aggtgccatt aaaagctaaa aaacgaaatt
    36001 atgtagcgtg gttatccgtt gccgcttctg ttgttgtaat gtttggagtt ggtttttatg
    36061 tgtacaatac ttcttttgca tcttccaacg aagatttagg aacctttgac aatccggaaa
    36121 tggctttcaa agaaacccaa aaagcattgg atttaatttc tacaaatgta aataccggaa
    36181 ttgaaagtat gagttatatt aatgagtttg aacaatcaaa aaataaagtt tttaaataaa
    36241 aagaaatcat gaaaaaaata aattataaat cgatgaaaaa agcaatatta accctagtgt
    36301 tattattcgt aaccaatgta ttctttgggc aacagtcgct ctttgataag tttgaagacc
    36361 aagataatgt tactgcagta attgtaaatc aaaagatgtt tcagttgatg agcaaaatga
    36421 aggttgacga caaagaaact caacagtatg ttaacttaat taagaagtta gacaatttaa
    36481 aagtatttgt aacaaacaat agtggcaaag caggcgaaat gaaatcggtt gccgataagt
    36541 atttgaaatc tgctaattta gaagaattaa tgcgcgttaa tcaaggcgga aaaagcgtga
    36601 aaatctatgt gcgttcgggc agtaaagaca ctttggtaaa agagttgttc atgtttgtgg
    36661 aaggtgcacc aaaagagcaa acagtactat tatcattaac tggcgaattt gatttaaacg
    36721 atatttcagt attgactgat aagatgaatt tacctggagg cgatgatttg aaaaaagcat
    36781 ctaaaggcaa aaaataaaac atcatgaaat tagtatattt gatagggttc accattgtaa
    36841 tgatgctgtt tagttgccat tcggagccaa gtttgcagcg ttattttgtt gacaatttag
    36901 aaaataaaga ttttgtttcg ttagatgtat cgtcaaatat tatcaatatt gacaaagcca
    36961 aattaacttc agaagaaaaa gatgctttgg aatcatttga aaaaatgaat atcttactat
    37021 tcaaaatgga cagtgcaaaa acgaaaacca aacaatttga aattgaatcg gctaaagtaa
    37081 aacaaattct aaaagggaaa gagtatcaag aactgatgaa agttggttcg ggaactaatg
    37141 ccgcttctat aagttttgtt ggtgatgaag ataaaattga tgaatttgta atttatgcca
    37201 aaggaaaaga cacaggtttt gccatagtta gaattcttgg tgatgatatg aatccaaata
    37261 atgtgatgtc aattttatcc attataaaga attcgggtat tgatatggag caattaaaac
    37321 cattagaagg gttgatgaaa taaaaaagaa ggttagttat tagaattgaa aaagtcctga
    37381 atttatcagg atttttttat ttcttgaagt atttaaatgg gacaaataac atcccaaaac
    37441 attcgccatc agttttaccg aggtgtttgt ggtgcatctt gtgtgctctt ctcacacctt
    37501 ttgcataaat attgttcgca tttcgaaaca atttaaagcg ttgatgaata aaaatatcat
    37561 gaacaataaa ataagcaact ccataagcaa atattcctaa tccgatagac aaaccgattt
    37621 caagtatttc tctttgccac aataagaaac aaccaatgct gactactgca taaaaaataa
    37681 aaaaagcatc gttgcgttca aaccaagaat catggtcttt tttgtgatgg tcggcatgga
    37741 gataccacaa aaagccatgc atcacatatt tatgagtaaa ccatgccata aattccataa
    37801 tacaaaaagt aagaacaaaa acaagaatat gtatccacat aatagtttaa attaaattta
    37861 aatgataatt aaaataacaa cgtgccaata aaccaaattt ttggtaatca gaaacacgaa
    37921 ttcgagtatt ttttatttcc aaagaaggtg tttttttcaa tttggacaat agttttttgt
    37981 agtaaatata agcagtataa actccaaatt ttgcttcaag tggtaattta acaattcctt
    38041 taaaacctgc ttcaaaatct gcctcaattt cacgaatgat ttcgtttttt gacgcttcgt
    38101 ccaatcgagt tagatctgta tttgggaaat aagttcggtt taaatcttca aaatcagctt
    38161 ttaaatctct taaaaaatta actttttgaa aagcagaacc caatcgcata gcggaatgtt
    38221 ctaattcttc aaacttttgt ttatcaccat ctacaaatac ttttaaacac atcaaaccca
    38281 ccacatcagc cgaaccataa atgtactcat tatattcttc aaacgagtta taggttgact
    38341 tgtgtaaatc aagtttcata cttttcataa atgccgcaat catggtgtgt ggaattttat
    38401 atttatgtac cgtgtgttga aaagaattta aaataggatt taaacttatt ttatttttta
    38461 aagcttcaga caaatctttt tcaaaaagca aaaaaagttc ctctttatta tactcgtgaa
    38521 aagagtcaac aatttcatct gcgaaacgaa caaaaccata aatattataa atgtcttgtc
    38581 tgatttttgg agacaacatt tttacagctg ccgaaaaaga ggtactgtat gatttagtaa
    38641 catgcctact acagtcgaat gatacggtgt caaaaataga tttcatagcg gtagttttta
    38701 atttttagta atcaattcag aaaccaattt tcctgaaatt aaagaaggtg gaactcctgg
    38761 tccaggaact gttaattgtc ccgtaaaata taaattattc acttttttac ttttcaactt
    38821 tggtcttaaa aaagcagttt gaagcaaggt atttgccaat ccgtaggcgt ttcctttata
    38881 agaattatat tctttaataa agtcattaac acaaaaagat tctttacata taatattttt
    38941 ttgaacagct tgttgcgtta tgttttcaaa acgggatata attttgttaa aatatttttc
    39001 tcttaattct ggagtatcct ctaaacctgg agctagcgga attaaaaaga ttccagcttc
    39061 acaatctttt ggtgcagcat tttggtctgt tttagatgga aaactggcat aaaacaatgg
    39121 ttcttcaggc cattttggat tgtcgtaaat atcggatgca tgaacatcaa aatcaacatc
    39181 aaaaaacaag gaatggtgtt caacattgta tattttttta ttaaaaccta cataaaatag
    39241 gagcgaagag ggagcaaata tttttttgtt ccaatatttt tcagaataaa cacggtagtt
    39301 ttcgtctaac aatgtttcac tatgatgata atcggcacca cttaaaataa cgtctgcttt
    39361 ttcttcaatt tcatttactg taatagagac agctttatta ttttgaacat ttatttttgt
    39421 aacattggca ttggttgtta tttttacacc aagctcaaga gccaattgtt ccatagctaa
    39481 aatcacactg tacattccgt ttttcggatg ccaagttccc aaaccaaaat cggcaaaatt
    39541 cataaaacta tagaaagaag gcgtgtccga aggttttgcc ccaagaaata acaccggaaa
    39601 ttctaaaatt tgcactaatt ttttgttttt aaactttttg cgaatatcat tagaaatgtt
    39661 tccaaaaaat tgatttactt tttttgcggt ttctaaagtt actaactcca atggtgaaac
    39721 gcctggtcga taaaccaaat ctttaattgc aatgttataa ttgtcttgtg cttctttgat
    39781 aaatgaagtt aattttttac cacttccttt ttcgatattt tcaaaagttt taacaatttc
    39841 agataaatta tcagctatag ttataaaatt atttattcca aaatagactt ggtatgcagg
    39901 tgctaatttt atcagttcat aataatccga tggtttttta tcgaaatcag caaaaaaacg
    39961 ttcaaaaaca tcgggcatcc aataccaagt tggaccaata tcaaaagtaa acccttcttt
    40021 ttttagttgt cttgctctac ctccaatagt gttatttttc tcaaatacag ttacctcgtt
    40081 tccttctttg gctaaataac aagcagcagc taaagaggaa aaaccagaac caattatgtt
    40141 tattttcttt ttcatttaat tttttctcaa aagagaaatt ataaattatg ggttaactct
    40201 aaaattgagt gaaacgattg atattgatta tctagtaatt ttggatctat attttctatc
    40261 attctaccga tgaaccaaaa tttatgctca aagtttttaa actcatcttt caattgtatc
    40321 acataattgt ttatatcact tggtgatggt tctacggtca tgtaacaaat gaatgtaatg
    40381 ttatcaaaat gaacattcat ctctttaata cattcaatag gaacactttc acctaaaaat
    40441 attgttttgt aaccctgtaa tagtatttca taatttagat acatcaagcc taattcatga
    40501 atttcattta agggaagata taaaatgaac acagtgtcat tttttattgg ttcttgcatc
    40561 tgaagctttt cggtattaat caatactttt tgtttgatta aataactaat aaagtgttcg
    40621 tgagccggac taatagtgtt attttgccac aagattccaa tttcttcaat caaaggaata
    40681 aaaacttcaa aaaagatttc tctaaaggtt ttttcattaa gtaaatcatt atacgttgaa
    40741 aaaaatagtg tctgatcaaa attcatcatc gccattttaa aagcactaat ggcatgactc
    40801 ttagcacttt ttttggttat aatttcgttt actatttgag gaatttgctc tttaggaaat
    40861 cgtgatattt tagaaatttt atacccatgg tcgtgtagga gtgttatatt taacaatttt
    40921 tgaagattgg caacatcata caaacggata ttagtatctg ttcgcattgg ttcgaggatg
    40981 ttgtatcttt tttcccaaat ccgaatggta tgtgctttta ttcctgaaag gttttctaag
    41041 tctttgatgc taaatacact cttaatgttg ttcatctttt ttatttaaaa gttaaacaaa
    41101 agtactaaaa atatatttag taaagcaaaa agtaacaaaa aaaaaagaca aacaaaatgt
    41161 ttgtctttaa aaaaagagtg accacgatgg gattcgaacc cacacgtcct ttcgaacacc
    41221 accccctcaa gatggcaagt ctaccagttt ctccacgtgg cccaaaacaa aaaaagtcat
    41281 ccgtataacg aacaaccttt tgtgacccga ctggggctcg aacccaggac cccatcatta
    41341 aaagtgatgt gctctaccaa ctgagctatc gagtcaaaag catataaaaa gaaaaaaatt
    41401 gtgacccgac tggggctcga acccaggacc ccatcattaa aagtgatgtg ctctaccaac
    41461 tgagctatcg agtcattgat tcaagaaaag tatttgataa tcacaaattt tgtgacccga
    41521 ctggggctcg aacccaggac cccatcatta aaagtgatgt gc
//
LOCUS       DAOOBH010000009        39461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_1320_length_39461_cov_6.353170, whole genome shotgun sequence.
ACCESSION   DAOOBH010000009 DAOOBH010000000
VERSION     DAOOBH010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 39461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39461
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1320_length_39461_cov_6.353170"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PK218_02300"
     CDS             <1..648
                     /locus_tag="PK218_02300"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPW97370.1"
                     /translation="LNQSTLKAIKSQMNPHFFYNALNTIQSYILANDKKQAVNYLSKF
                     SSLTRTILEMSEKETVTVAEEIKTLSFYLDIEKARFNDDFEYEIIINEATLEQIKIPS
                     LLIQPYVENAIKHGLLHKNGAKKLTIVFEENGNYLDISIIDNGIGRTKSEELKLLRKN
                     PHQSFATEAMQQKIDLLNKHKINKISIEIIDNYSTQKTPKGTTVLIKIPLDFYYS"
     gene            645..1385
                     /locus_tag="PK218_02305"
     CDS             645..1385
                     /locus_tag="PK218_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPW97371.1"
                     /translation="MKAIIIDDEKRARVSLQLLMEEYCPEVTLVAECENLPEGVKAIH
                     KHKPDLVLLDIEMPGHSGLELLDFFNENEITFAIIFTTAYNEFAIQAFKLSALDYLLK
                     PIIPEELTNAIARLERQKAVQFKALAENIQSENFEKIAVPSGNCLILLKISDIIYIKG
                     EGSYSEVFMKNNVKHLVSRNLKNFEELLSNDKQFLRTHKSYIVNFDEVTSYNKSDGGW
                     LELQDNISVPVSSEKVNLIMERIKIVKR"
     gene            complement(1375..2625)
                     /gene="aroA"
                     /locus_tag="PK218_02310"
     CDS             complement(1375..2625)
                     /gene="aroA"
                     /locus_tag="PK218_02310"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPW97372.1"
                     /translation="MNLELQKSKIVNHQSSIKITGSKSETNRLLLLQALYPNIELENV
                     SKSDDSEVMLLALTQNLQLSTYNLIDVHHAGTAMRFLTAYFAIQQNKEIILTGSSRMK
                     DRPIGIVVDALNQLGADVSYKGKKGFPPLKINGKKLIKNKVSLSANVSSQYISALLLI
                     APKLENGLELTLEGEITSVPYINMTLALLNEIGVKTSFENNTIKVNHQPSIINRQRIT
                     IESDWSSASYWYSIVALSPARTKIKLSSFKENSLQGDSVLIEIYKNFGVQTSFGNNEI
                     ILEKSNYYHQSVILDLSKSPDIAQTIVVTSFALGLGCRLNGLHTLKIKETDRLEALRK
                     ELTKLGAMILTNDSGLILAPSTRIKDNISIKTYQDHRMAMAFAPLALKTNLIIEDAEV
                     VSKSYPTFWEDLKQVGFQIEEFNA"
     gene            complement(2672..2998)
                     /locus_tag="PK218_02315"
     CDS             complement(2672..2998)
                     /locus_tag="PK218_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HPW97373.1"
                     /translation="MNLKNSQLEVDNWIKNHGVRYFNELTNMAQLTEEVGEVARIIAR
                     RYGEQSEKESDKNKDLGEELADVVFVVLCLANQTGIDLQAAFDKKLDLKTNRDHERHQ
                     NNEKLK"
     gene            complement(3001..5001)
                     /locus_tag="PK218_02320"
     CDS             complement(3001..5001)
                     /locus_tag="PK218_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPW97374.1"
                     /translation="MKKSLLLLLFIGINMIAQNRELGKVTIQELEEKICPTDSSAVAA
                     ILFSIGEVHFEFIEGKGFVLNTNVKTKIKIYKKEGYDWANKTVRYYIGGSTKERVSFK
                     NELTYNLVNNKIEKSKIKSDGEFDEKVNDFWGRKKITMPNVKVGSIIEYEYNLVSESL
                     GSLDEWFFQSSIPVLYSEYRVYTPEYYTYTPVFKGFLQPKLIEGAKRITSRSTSKERS
                     GGLRTSTSFEQEEFSYDELRRTYIVENAPALKDEAYVNNIRNYVSSVVFELSGVKQKN
                     LSYKDLSNTWEGVVKTIYDNDNFGNELNKTGYFEKDIDAILAGLTMPEEKLIALFTFV
                     KSKMNWNEYYSYTCNTGVRKAYQEKTGNVAEINLMLTSMLRYAGFEANPVLISTRSNG
                     ISLFPSRTAFDYVIVGVEINNQVTLLDATAKYSTPNILPIRDLNWFGRLIRKDGTSTQ
                     IDLMPKFNSKEMINLMAEINAQGEVLGKVRDQYFDYNAYNYRNTYNGVTKETMIEKIE
                     KRHQGLEISEYDVQNSAELSKPIIENYSFSSNNFVEIIGEKMYFSPLLHFTQTENPFK
                     QETRLYPIDFVYPNQDKYSVSIKIPEGYSVESLPSSKALGLPDDLGNFKYSISNTGNQ
                     IQLMYSFDINTPIISSDMYEVVKTFYKEMITKQTEKIVLKKV"
     gene            complement(5008..6915)
                     /locus_tag="PK218_02325"
     CDS             complement(5008..6915)
                     /locus_tag="PK218_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPW97375.1"
                     /translation="MKSTIIILFSLFFSNLFFAQKSEYTVTTISDSLKNNANAVIRLS
                     DITITISSQNAMIITTKVVTTVLNEFGLRNLNLSENYDKNRKVSKIEAIAYDAFGKEL
                     KSFRKKDFKDVSVADGISIFNDNRVLYLDYTPTVYPFTMVYESQIETSNTAFIPSWSP
                     VDDYYVSTENTKLTISFKPELKVNFKELNFSKKYLINKTSTNGQINFYSANVQASKKE
                     ELAPVFSKVFPLVIFGLDKFHLEGINGVANSWNEMGKWFYDNILNGTTELPEETKVKV
                     KNLVANETDPLKKAEIIYKYVQDKTRYVSIQEGIGGWKPMNAKDVDKLGYGDCKALTN
                     YTRALLNEVGVQSFYSRLYGQNTNKEIYSDLVSFQSNHVILAIPNKNDYLWLECTSQI
                     QPFGFQGGFTGNRNAFLIKPDGGEIVRTKTFDENDNLKQIKGSYKISDDGKITATLKI
                     ESFGLQYDNQFQKERLSKEDQIKNYKEEYSNINNISFSKINFINDKNKIKFTEEFELE
                     AKNYAQNVNGKLMFAINAFDQSSYVPKKHRTRELPFEIESGYTDEEEITVTLPYGYVV
                     EAKPNGAELKTEFGSYKIDFIISNNKNIICKRKLIINKGFYESSKFENYRKFRETIAK
                     TDNSKVVISKT"
     gene            complement(6965..7417)
                     /gene="dtd"
                     /locus_tag="PK218_02330"
     CDS             complement(6965..7417)
                     /gene="dtd"
                     /locus_tag="PK218_02330"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPW97376.1"
                     /translation="MKAVIQRTTSASVTIESKIVAEIQNGLLILVGIEELDNHEDVNW
                     LCSKIANLRIFSDENQVMNCSVKDINGDIIVVSQFTLHASTKKGNRPSYIKAAKPEIA
                     IPLYEKFIIQMETEMGKKIQTGRFGADMKVALTNDGPVTIIIDTKNKE"
     gene            complement(7428..8039)
                     /locus_tag="PK218_02335"
     CDS             complement(7428..8039)
                     /locus_tag="PK218_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HPW97377.1"
                     /translation="MKKVASISIGILALITVAIQFYLMIENRVASITETVIRFFSFFT
                     ILTNLFVGIYFTLLFINIKKSIIPFINKPGNLSSITVYITIVGLVYQFALRNVWEPKG
                     LQKLVDELLHSIIPLLVILFWYFYENKSAIMYKSVLLWLIYPLVYLFYILGRGFFSGF
                     YPYPFVNVAEIGLTKVMTNSIILLVLFTVISTLFIKIGHKLTK"
     gene            complement(8058..>8446)
                     /gene="rsgA"
                     /locus_tag="PK218_02340"
     CDS             complement(8058..>8446)
                     /gene="rsgA"
                     /locus_tag="PK218_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPW97378.1"
                     /translation="PNLNLKTKDISEQSKQGQHTTTFAEMYDLSFDAKIIDTPGIKGF
                     GIVDMERTEISDYFPEFFVLKEQCKFNNCLHKEEPNCAVKKALEENKIAWSRYNSYLK
                     ILEGDEEHYRTDNYDEERKASDETRK"
     assembly_gap    8447..8456
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8457..9040)
                     /gene="rsgA"
                     /locus_tag="PK218_02345"
     CDS             complement(<8457..9040)
                     /gene="rsgA"
                     /locus_tag="PK218_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPW97379.1"
                     /translation="MTGIVYKSTGSSYSVKTNDNQVFECRIKGKFRMKGIKSTNPIAV
                     GDIVDFELDKSTDETIGQIHNIQERKNYIVRKSVNLSKQTHIIASNIDVVFLLITINN
                     PPTTTSFIDRFLVTAEAYGIEAILVFNKIDTYDDAMEDEQLFLQYVYSQIGYKCLKVS
                     AKETIGIDALKNEMIGKVSMFSGHSGVGKSTLVNA"
     gene            complement(9127..9651)
                     /locus_tag="PK218_02350"
     CDS             complement(9127..9651)
                     /locus_tag="PK218_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97380.1"
                     /translation="MKKIILVLTLVFGLNLAKAQDVVTLHNGSTLEGKVSSTDGATIT
                     FTYKGEDVSTKISKIAINSITYASGRKQVVTEKLVLEGKDAFQKVILTKNPEDVEGLT
                     RIGEVKGKTAFISYRTGANADKKSEERLKEDCYKLGGVVVLVTEDKDYAVGKTYQKGL
                     GSSQTRMIGVAYKY"
     gene            complement(9737..10819)
                     /locus_tag="PK218_02355"
     CDS             complement(9737..10819)
                     /locus_tag="PK218_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HPW97381.1"
                     /translation="MKKEEQHTNWLDDFKLTHPLVIAGPCSAETEEQVLKIAHELKDS
                     DVSIFRAGIWKPRTRPGGFEGVGAIGLKWLQKAKAQTGLLMAIEVANANHVKLAIEHD
                     IDVLWIGARTTVNPFAVQEIADALNGTDKIVLVKNPVNPDLALWIGALERLENAGIKK
                     IGAIHRGFSTYEKTKYRNNPEWQIAIDLQSRFPVLPLLCDPSHITGKRNLIQEVSQQA
                     LDLNYDGLMIETHIDPDNAWSDAAQQVTPATLKQIIKELRVRKVSDDADDFTKEMNKK
                     RTQIDVFDTKIVEILGKRMMIAEEIGLLKKEKNVAILQNKRWNEVLNKMISEGENKGL
                     SEEFILKLFKAIHQESISHQEKIINN"
     gene            10880..11779
                     /gene="gldA"
                     /locus_tag="PK218_02360"
     CDS             10880..11779
                     /gene="gldA"
                     /locus_tag="PK218_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HPW97382.1"
                     /translation="MSIEVQNISKSYGAQKALDQISFSVKKGEIVGFLGPNGAGKSTL
                     MKILTTFIAADEGKASVNGNDVVENSKSVQQSVGYLPEHNPLYLDLYVREYLAFNADV
                     YKISKSRIEEVIELTGLTPESHKKIGQLSKGYRQRVGLATALLHNPEVLILDEPTTGL
                     DPNQLVEIRNVIKNVGKDKTVFLSTHIMQEVEAICDRVIIIDKGKIVTDKKLTKLVSD
                     EAEQIIEVEFDKPISESILNTLPNLKSVKNPQNNIWELTFTTNDDMRSIVFDFAKDND
                     LKTLQISSRSKNLEQIFREKTKK"
     gene            complement(11780..12529)
                     /locus_tag="PK218_02365"
     CDS             complement(11780..12529)
                     /locus_tag="PK218_02365"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPW97383.1"
                     /translation="MKKSLLLVLVILLLNSCEKPSDCIESSGATVVKEIAVSAFSKIK
                     VYRGIAIIITQGPNYKVEIEAGENFINDISVTQNGDQLVFKDQTSCNWVRDYGQTKVY
                     ITTPTLTEVYSKTDRDIRSNGVLTFPDLSILSLDKDADGENGAGTGDFYVEVANNSLT
                     ISNNNVSRFYLSGTTNLASLNFYYGDGRIEAQNLIAQQINVFHRGSNDMYVTPVQSIE
                     GVLNSTGDLILTTTPPIVNVQELYQGRVVYP"
     gene            complement(12540..13628)
                     /locus_tag="PK218_02370"
     CDS             complement(12540..13628)
                     /locus_tag="PK218_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HPW97384.1"
                     /translation="MRSFFFLVSCLFSSLCFSQNNNFAVEASVLRGNILPHTEDMYQL
                     VNGHPDGLMVRFLKRTDGSKEWHQAYNYPDYGAYFLYQDFHSAPLGKNYAIGGLYNFY
                     FWKRRLQLSIGQGIALATEPYDKVANSKNKAFGTKFMGNTNFGFAYDHQNLVKNIGFH
                     AGILFTHYSNGRFKSPNSGINTYLLNVGVNYDFESVVIREVDTSSIKKSYREPLHYNV
                     VFRTGVNESPIIRSGQHPFYHIGFYVDKRLNRKSTLQLGTELFLTESIRDYIYYYSVA
                     YPDKNIDADTDYKRVGVFIGHELMINRLSLEAQLGVYVYQPFKKDIPVYDRVGMKYRI
                     TDKIFGGFSIKTHLFLAEALEFGIGYRI"
     gene            complement(13736..14692)
                     /gene="metF"
                     /locus_tag="PK218_02375"
     CDS             complement(13736..14692)
                     /gene="metF"
                     /locus_tag="PK218_02375"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HPW97385.1"
                     /translation="MKVTQHIENANGKPLFSFEILPPLKGQNIQSIFDGIDPLMEFKP
                     PFIDVTYHREEYEFKELPSGLLQKKIVKKRPGTVGICAGIQNKYEVDAIPHILCGGFT
                     KEDTENLLIDLDFLGIDNVVALRGDAMKNETYFKPEKEGNEFASDLVLQITNLNNGIY
                     LDEDLKNSAETNFCIGIAGYPEKHMEAPSIDSDIHFLKQKIKNGAHYIVTQMFFDNKK
                     FFAFVEKCRLAGITVPIIPGLKPIATKKQLNLIPHRFKVDLPDDLVMEVVKAKDNDAV
                     KQIGIDWCLQQSKELLAAGIPCLHYYSMGKAENIKAIAKEIF"
     gene            complement(14763..17438)
                     /gene="metH"
                     /locus_tag="PK218_02380"
     CDS             complement(14763..17438)
                     /gene="metH"
                     /locus_tag="PK218_02380"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPW97386.1"
                     /translation="MSETKYLKLSGLEPLVITPETNFVNVGERTNVTGSRKFLRLIKE
                     EKYDEALSVARDQVDGGAQIIDINMDEGMLDGVYAMTKFLNLIAAEPDIARVPIMIDS
                     SKWEIIEAGLKVVQGKAVVNSISLKEGKEAFVHHAKLIKRYGAAVIVMAFDENGQADN
                     YERRIEICKRSYDILVDEVHFPAEDIIFDPNIFPVATGMDEHKLNALDFFRATKWIRE
                     NLPYAHVSGGVSNVSFSFRGNDKVREAMHSAFLFHAIKNGMSMGIVNPEMLEIYDEID
                     KNLLEHVEDVLLNRREDATERLLDLAESFKGDFKSNEKEIAEWRNGSIQERLTHSLVK
                     GIDEFIELDVEEARANSEKAIEVIEINLMEGMNVVGDLFGSGKMFLPQVVKSARVMKK
                     AVAYLLPFIEAQKTGKSSSAGKVLMATVKGDVHDIGKNIVAVVLACNNFEIIDLGVMV
                     PPEKIIAAAIENNVDIIGLSGLITPSLDEMVYLAKEMDKLNISIPIMIGGATTSRAHT
                     AVKIAPQYKETVVHVNDASRAVTVASNLLQSETKENYVKTIREDYDKLREGYLNRSRD
                     KNYLTIEQARKNKIQIDWDNFTPVKPNFIGTKIIDVELSQLVDFIDWTPFFQSWELFG
                     KYPAILTDEIVGEHASNLFTDAQKMLNQIVSEKWLTAKGILGIFPANSVNDDDILIQP
                     TTYNLQPTTFLTLRQQSQKTAGAPNKALADYIAPKNSGKQDYMGCFCVTTGFGLDEIA
                     NQYVKDLDDYNSIMIKALGDRLAEAFAEYLHLKVRKEIWGYASDENLNNQELIKEAYK
                     GIRPAPGYPACPDHLEKPTIWKLLNVEQEIGVKLTESMAMWPASSVSGYYFANPESKY
                     FGLGKIKEDQVIDYAKRRGISTEVATKWLSPNIAE"
     gene            complement(17660..18646)
                     /locus_tag="PK218_02385"
     CDS             complement(17660..18646)
                     /locus_tag="PK218_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPW97387.1"
                     /translation="MSKIQEAIQERILVLDGAMGTMLQRNNFSEEDFRGERFKDFPHP
                     LKGNNDLLSITQPEAVKQVHRLYFQAGADIVETNTFSGTTIGMADYHMEELVYELNYQ
                     SAKIAREVADEFTDKPRFVAGSIGPTNRTASMSPDVNDPGYRAVTFDDLRIAYRQQVE
                     ALIDGGCDVLLVETIFDTLNAKAALFAIEEVKEERNIDIPVMVSGTITDASGRTLSGQ
                     TVEAFLISISHIPLLSIGFNCALGADQLKPYLKRLAHNTQLNISAHPNAGLPNAFGEY
                     DQTPLEMQALIRDYLQDNLINIIGGCCGTTPEHIKAIADVVKEFKPRTLELA"
     regulatory      complement(18703..18805)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            19082..20332
                     /gene="metK"
                     /locus_tag="PK218_02390"
     CDS             19082..20332
                     /gene="metK"
                     /locus_tag="PK218_02390"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPW97388.1"
                     /translation="MAYLFTSESVSEGHPDKVADQISDALIDNFLAFDPESKVACETL
                     VTTGQVILAGEVKSTTYLDVQQIAREVIRKIGYTKSEYMFEADSCGVLSAIHEQSSDI
                     NQGVDRASKEEQGAGDQGMMFGYATNETENYMPLALDLSHALLIELANLRRENNEIKY
                     LRPDAKSQVTLEYSDDNKPQRIDAIVISTQHDDFAPEAEMLAKIKSDLVSILIPRIKA
                     KYPQYAHLFNDDITYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGK
                     DPSKVDRSAAYATRHIAKNLVAAGLCDEVLVQVSYAIGVAKPTSINVVTYGTSKVNLT
                     DGEISKKVEAIFDMRPYFIEQRLKLRNPIYSETAAYGHMGRTPETVTKTFQLPNGAPK
                     TVTVELFTWEKLDYVDQVKAAFNI"
     gene            20407..21765
                     /locus_tag="PK218_02395"
     CDS             20407..21765
                     /locus_tag="PK218_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97389.1"
                     /translation="MIELLNRIENRLKFRPSYYLLFAILNGAVLMNFHNYLFKHSIGI
                     VFLFCFVIYTIYKPKNWISVIGSCLFSVYYLLDFFPRIGNHSSIILFTSLTILLLFIF
                     KLFSKKEVLNSKLLAYFFRIFTVTVYFYTGFHKLNTDYFNTCVSCVNEINEYTLSNIF
                     GFPIKIGDSLSRFFQIIALIVECIVPFGIMHYKTRSYSVLLLLLFHSYLSLSVFADFS
                     AVGLFLLLGCFIDFEENSFSKKTIFYIRLYLIMIMISVISPIFLSRISVQTSVFPFIK
                     GFIFLVGYLSFFIYFFRNHDAKRYSFRRSYFMPLFILCISISFWTLKGYIGLGNAGNL
                     TMFSNLVTEKSMNNHLLIDTKKTKLFNFEEDNVYIIKMDNPNIKENYTGYKLPIVEFR
                     FLVNYWSKNFDKPIPCILVYKNKKYTFKDIKTSEFKNSKWWYKYLNFRKIQTTSPNEC
                     RW"
     gene            complement(21791..24523)
                     /locus_tag="PK218_02400"
     CDS             complement(21791..24523)
                     /locus_tag="PK218_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPW97390.1"
                     /translation="MKKLLLFILTITSLLSFSQNVRFEGIIQDTNKQPLEMANIMAMN
                     QATKGMDSYAITNDKGRFVLNLKPNTTYTVKLSYLGMQNKELTITTESENITQNITME
                     SGGIDLEGVEIVREMPVSIKGDTIVYNADSFKNGTERKLEDVLKKLPGVEVNADGEVE
                     VEGKKVTKLMVEGKDFFDGDTKLGVKNIPADAIDKVQVLRNFNENSILKGVENNQDNL
                     AMNIKLKDGKKNFWFGDVTAGGGMDDKNKFDRYIINPKLFYYNPKYSINVITNFNNIG
                     ELPLTIQDYFKFTGGFRSMMSKGGSSFNVLSNDLGVSLLRNNRAKEIDTKFGATNFSY
                     NPSKTWTLSGFGILSSSVTAIETVTNTNYLKPNSTEVASTENRNQIAHQNSRLGLMKL
                     SSLYKPNANFQMDYDVLSKFSKQDEDNSLLREVIDEDNISTTETILSNKTQNPVTINQ
                     NLSFYYTPTSKHVFALEMQHLYQEEDPFYNANLQTQPFNLSGYIAGQNRNDINQDRFV
                     KTNKIDTKLDYYYMVTPKSNINVTFGNTYSYQNFNSRIFQILDNQDINNLDDSENTND
                     VNYRFNDAFLGLHYKLLTGKFTLTPGFSIHNYSLNNTQLGSSYKQNFGRILPDFLAIY
                     QIKKAESLTYNFSFNNNFTDINRLAEGYVFNNYNSLTSGNRTLENAIQQSHSLRYFKY
                     NMFNFENISAFINYNRTVDAVKNRALFNNVNQVSSPINSAFADESLSGNLRYGRSFFK
                     YYKASISANLNWSKYNNIQVDRITLEDVLLTNESFSQTYDFDISTNFKNFPNLELGYG
                     YSVNQYPNDTFYTDRPNAKLEYFFLKSFSFVSEYEYFHYYNKDKSVENEYDFLSASLI
                     FQKTKDSHFEYKVSVTNLLNTKALKDNSFSQFSTSDSQYTIQPRYLIFTLKYNL"
     gene            complement(24544..25380)
                     /locus_tag="PK218_02405"
     CDS             complement(24544..25380)
                     /locus_tag="PK218_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HPW97391.1"
                     /translation="MKKIVLPFLLLGTLLSNAQDFQGKAEYMSKYIFKKDNIKSDKVD
                     ENPDLTAALKEALNKSSQKTFSLTFNKYEAVYEEVEQLQQPQAPSNGMSISIKFSGAG
                     KKYINIKDKTTLAEDEIFGKEFLISEPLEKANWKLVNESKKIGDYNCFKAELIIPVTE
                     NEKEAYQEFLKKEKVKPALFKMDEPKDKVIVAWYTPEIPVSFGPNNYWGLPGLILEVN
                     EENNIILCSKIVLNSKDKSEIKKPKNGEKVSQKRFDEIQKKKMDSMKDEDGNVIFQTI
                     EH"
     gene            complement(25390..26283)
                     /locus_tag="PK218_02410"
     CDS             complement(25390..26283)
                     /locus_tag="PK218_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HPW97392.1"
                     /translation="MKNLAIITFFLFTFVAVAQKEFQGMAVYESKTSTSDFKSRMEGN
                     KNITPDMQKMIEERMKKMFEKTFILNFDKSASIYKEEEKLDAPNQAGGMRMMSSFMGG
                     GGTYYKNVKEKMYTVDKEFMGKEFLVKDSLVTYNWQMNGETRVIGGYNCFKATAVIPA
                     SKTDFRNFRPKKEDEKKDEKAADAKKEDEKEKKTSFLDEIDLPKEITITAWYTPEIPV
                     SQGPEGYWGLPGLILEINDGKTVTLCSKIVLNAKEKAEIKAPKNGKVVSQKEYDETVI
                     SKMEEMREMGGGRGRGGFGPR"
     gene            26404..27192
                     /locus_tag="PK218_02415"
     CDS             26404..27192
                     /locus_tag="PK218_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97393.1"
                     /translation="MIKKVIFILFLLVCPFSMSQKLTTTLVNSTPLAADSFLGIDTMG
                     NYYFIKDNVFTKKNASQTWQYKNVALGKITSVDILNPLKIIIFYENFNTTILLDAQLN
                     EILQKNFSIALKETLVSHVGLASNNRLWLYNNLTNSIELYDYTSNASQTVGQPLTQKI
                     VYAQTNFNTFYWIDEMNNLYTCDVFGKVTLMCNVPAYEKIQIIDGERLLFSTNNKLYL
                     LNNTLKSIIEIEIVENSFENFYCKDQNLAIFTNQQISNYKIILP"
     gene            27192..27806
                     /locus_tag="PK218_02420"
     CDS             27192..27806
                     /locus_tag="PK218_02420"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPW97394.1"
                     /translation="MHIAIAGNIGAGKTSLTRSLAKHFKWEPHFEDVVDNPYLDDFYH
                     QMERWSFNLQIYFLNSRFRQILQIRESGKNIIQDRTIYEDAHIFAPNLHAMGLMTNRD
                     FQNYSSLFELMENLVGPPDLLIYLRSSIPNLVGQIHKRGRDYENTISIDYLSRLNERY
                     EAWVHSYDKGKLLIIDVDNIDFVNNPEDLGMVINKIDAEINGLF"
     gene            27865..28455
                     /locus_tag="PK218_02425"
     CDS             27865..28455
                     /locus_tag="PK218_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPW97395.1"
                     /translation="MPLVNPLSPEQDQETKELAVFFNETLGFCPNSVLTMQHRPAISK
                     AFINLNKAVMENHGRVTSALKRMIGWVTSNAAGCRYCQAHTIRAAERYGANQEQLDNI
                     WEYASHPAFSDAERAALNFALAASVIPNAVNEAIKNDLYSHWNEGEIVEMLGVISLFG
                     YLNRWNDSMGTTIEDGAVESAEQYLGKTGWEKGKHQ"
     gene            complement(28669..29523)
                     /gene="accD"
                     /locus_tag="PK218_02430"
     CDS             complement(28669..29523)
                     /gene="accD"
                     /locus_tag="PK218_02430"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPW97396.1"
                     /translation="MAWFKRKEKGITTATEDKKDVPKGLWYKSPTGKIIDTDELAKNL
                     WVSPEDDFHVRIGSKEYFEILFDNNEFKELDAKMTSKDPLHFVDTKKYSERLKDVQEK
                     TKLKDAVRTGVGKSKGKDLVVCCMDFAFIGGSMGAVVGEKIARGIDYSIKHKLPFVMI
                     SKSGGARMMEAAYSLMQLAKTSAKLAQLADAQIPYISLCTDPTTGGTTASYAMLGDIN
                     ISEPGALIGFAGPRVVRDTTGKDLPEGFQTAEFLLEHGFLDFISHRKEMKNKINLYID
                     LILNQEIR"
     gene            complement(29587..30654)
                     /gene="fbaA"
                     /locus_tag="PK218_02435"
     CDS             complement(29587..30654)
                     /gene="fbaA"
                     /locus_tag="PK218_02435"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPW97397.1"
                     /translation="MAHNIKPGVATGDEVQAIFAYAKEKGFALPAVNVIGSSSINSVL
                     ETAAKLNAPVIIQFSNGGAHFNAGKGLSNENEKAAIAGGVAGAKHIHSLAEAYGATVI
                     LHTDHCAKKLLPWIDGLLDASEKHFAETGKPLYSSHMIDLSEEPIEENIEICKEYLAR
                     MSKMGMTLEIELGITGGEEDGVDNSDVDSSKLYTQPEEVAYAYEELMKVSPRFTIAAA
                     FGNVHGVYKPGNVKLTPKILKNSQDYVQNKYNTGNNPVDFVFHGGSGSTIEEIREAIG
                     YGVIKMNIDTDLQFAFTEGIRDYMTSKIDYLRTQIGSPEGSDSPNKKHYDPRKWIREG
                     EMTFNKRLEQAFADLNNVNTL"
     gene            complement(30706..33252)
                     /locus_tag="PK218_02440"
     CDS             complement(30706..33252)
                     /locus_tag="PK218_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPW97398.1"
                     /translation="MKFNCTKIVFIILSGLFLFSCNTTKRVPDGKKLLVNNTIEVDEQ
                     KEKSEEVFNQLYQKQNSSILGYKLRLNIYNLAKQKTDSIYRAKFINNPKKYYRKSKWL
                     SRKQVKRLGESFWYSGIHNFLMKTGEAPVILDTASTKKSLRRLRLYYSNIGYFDAKAK
                     FTIDTIAAKKAKLNYSVTKGKPYIIDSIATIITTKVLDSIYQEKKNNSYIKVGDQFNR
                     NNFENERNRITEDFRNNGAFKFQQNYINYEIDTIKRGHKPKITILIDDEVSRVNDSTV
                     ISPFKLYKINRVNIFTDHSTANPNAKITDSAVYKNFNIYSVNKLKYRLKSITNGVFIA
                     KDNYFSDFKTAITSRYLTNLKVFNYPLIQYVEDKNDQNGLIANIYLTPRKKYTFGFST
                     DFTHSNIQDFGISGTTFLSIRNVFNGTETFDLGFRGNIGSSRDLANPNDNFFNISEIG
                     IDAKLNFPKLILPFKTEKIIPKRMIPYTTLSVGYAKQRNVGLDKQNFTSSFTYNWTPK
                     RNVSFKFDLINIQFVKNLNPTNYFNIYTSSYNALNDIAQNYPVSTDFLDANGNLKIDD
                     GTSGFLVAAFTDPSLNLSTVDLKSVLSILERRVRLTENNLIFSSNITYSKTSKKDIFD
                     NNFYAFKTKFESAGNLLSLFSRISKQLKSQSGANTFFEVEYSQYIKSELEYIRHWGLS
                     SKQVFAVKGFFGIAIPYGNSNSIPFSRSYFGGGSNDIRAWQSYSLGPGSSESILDFNE
                     ANMKLTLSSEFRFNVFGNLYSAVFVDAGNIWNVLDNISNEKSVFSGVNSLKDIAVGTG
                     FGLRYDFNFFVLRTDFGFKTYNPANPENEKWFKDIRFDKSVLNIGINYPF"
     gene            33287..34015
                     /locus_tag="PK218_02445"
     CDS             33287..34015
                     /locus_tag="PK218_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPW97399.1"
                     /translation="MVSKNQIKLINSLQQKKYRIEHQLFIAEGVKVIQELLQSNSVLE
                     HLFATNDSFSTFKVQAKTEITTQEMKKISGLSSPSSCLALFRIPKPTNINPMGLIVAL
                     DAIRDPGNLGTIVRLCDWFGVTQIVCSNSTVDLYNPKVVQATMGSIARVNVNYLDLKT
                     FIIESNLPVFGTFMDGATIYNEKLPKEGIIVFGNEANGISLEIEKNVKNKIAIPQFGS
                     IQKTESLNVATAMAIVLSEFRRND"
     gene            complement(34012..34716)
                     /locus_tag="PK218_02450"
     CDS             complement(34012..34716)
                     /locus_tag="PK218_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPW97400.1"
                     /translation="MKKLFFLILISTTSQLHAQFGKGMFSKDPIVNLENFDKQRVYWG
                     YFLGFSSFDFKTDYKVEQNNIDIKGQTGFNVGLVGDLRLHEYVSLRFEPGLYYASRTI
                     TYSQFTNDRDGLREVKATYINFPLLLKFSSLRTGNIRPYLVGGVSATLNLSSNSKSDD
                     DNLQQRFRVKSWTQNYELGFGVDLYFEYFKFSPSIRGVFGLNDELIRDNDPNSPWTGN
                     IQSMKTRAILINFTFH"
     gene            complement(34740..35471)
                     /gene="ubiE"
                     /locus_tag="PK218_02455"
     CDS             complement(34740..35471)
                     /gene="ubiE"
                     /locus_tag="PK218_02455"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HPW97401.1"
                     /translation="MSKQITPYKDSDLGKKEQVTQMFDNISGNYDELNRVISLGIDVK
                     WRKKVVQLVADKNPKTILDIATGTGDLVIMMADSTAEKIVGLDLSAGMLEIGKQKINQ
                     KNLSNKIEMIQADSENIPFEDNTFDAITVSFGIRNFETLEKGLAEIYRVLKPNGIFVI
                     LETSVPTKFPFKQGYYFYSKMILPIIGKIFSKDKSAYSYLSTSAANFPFGEKLNNILR
                     KVSFIECKALPQTFGVATIYTATKK"
     gene            complement(35522..36010)
                     /locus_tag="PK218_02460"
     CDS             complement(35522..36010)
                     /locus_tag="PK218_02460"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPW97402.1"
                     /translation="MITLIAAIGENNELGKDNQLIWHLPDDFKRFKAITSGHFIIMGR
                     KTFESFPKPLPNRTHVIVTRQKNYAPKDCIIVNSIKDGIAACPIDEAIYIIGGGEIYN
                     QSINLADKLELTRVHHTFEADTYFPKLNMDLWQLEFEEFHPKDNKHNFDFTFLTYSRK
                     TE"
     gene            complement(36007..36345)
                     /locus_tag="PK218_02465"
     CDS             complement(36007..36345)
                     /locus_tag="PK218_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HPW97403.1"
                     /translation="MEKDQHELYEYARNRIKQKKRLYFHFVLFILGSIFLIVANNLLG
                     FYPETKWWIWAVTIWGFIFVLHIIKVFITDAFMNKNWERTQIDKLIQLQTKKIEKLNA
                     DLEKKATTNE"
     gene            36584..36880
                     /locus_tag="PK218_02470"
     CDS             36584..36880
                     /locus_tag="PK218_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HPW97404.1"
                     /translation="MAIKKQFIKSKPVCKVTFSIEAKEANTAAVVGDFNNWNPSEGEL
                     SKLKNGTFKGLFEVPKDASYEYKYVVDGTYVNDPEADGYVYNDFAGAENSVVTV"
     gene            complement(36947..37894)
                     /locus_tag="PK218_02475"
     CDS             complement(36947..37894)
                     /locus_tag="PK218_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HPW97405.1"
                     /translation="MKKTILLLLFSSISVSVGCYLNTKPKKNPAEKMQDFIINISDYT
                     RKINPNFIIIPQNGAELAFENGKTDKKLDQKFLEAIDGFGIEELFYNGELKPDTYRID
                     LLSKIKESETIIVSEFVKDTALVKDSYERNEKSGFLCFTRTEENYHYTLIPTKVHNEN
                     KNNVEKLSDIKNFLYIINNSNYYSKSAFITALKNTNYDLIFIDLYHNGVQFNKEEIEK
                     LKIKKNGGKRLVVGYMNIGAAEKYRTYWRRDWKINNPKWLKKPYKDYDQEIWVEYWNS
                     EWQDIIYGNDNSYAKKIVDTGFDGVYLDNVEAYYFLYND"
     gene            complement(37905..38729)
                     /locus_tag="PK218_02480"
     CDS             complement(37905..38729)
                     /locus_tag="PK218_02480"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPW97406.1"
                     /translation="MKQYHDLMQHVLENGCQKGDRTGTGTKSVFGYQMRFDLNEGFPM
                     VTTKKLHLKSIVYELLWFLKGDTNISYLKENGVKIWDEWADENGDLGPVYGHQWRNWN
                     SEEIDQITELIETLKNNPNSRRMLVSAWNPSVMPDTSKTFSENVANGKAALPPCHAFF
                     QFYVANGKLSCQLYQRSADIFLGVPFNIASYALLTMMIAQVCNLQVGDFIHTFGDAHI
                     YNNHFEQVELQLSRPILPLPKMILNPEVKNIFDFTFEDFTLVDYNPHPHIKGVVAI"
     gene            complement(38832..39449)
                     /locus_tag="PK218_02485"
     CDS             complement(38832..39449)
                     /locus_tag="PK218_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPW97407.1"
                     /translation="MSLVKLSIKGISYSQTQNGAYALILNEVDGERKLPIVIGAFEAQ
                     AIAIALEKEIKPPRPLTHDLFKSFADRFDIVVKQVIIHKLVDGVFYSSIICERDKIEE
                     IIDARTSDAIALALRFQAPIFTYKNILDKAGIYLNNPNETEVEDDGVLSTPETFGLDS
                     TDIDSSESYSKLSLSELHSSLEAAVQDEDYEKAARIRDEISKRES"
BASE COUNT        12438 a         6739 c         5844 g        14430 t
ORIGIN      
        1 cttaaccaat cgacattaaa ggcaataaaa tcgcaaatga atcctcattt tttctataat
       61 gccttgaata caattcaatc gtatattttg gcaaatgaca aaaaacaagc tgtaaattat
      121 ctttcaaaat tttcatcttt aacccgaaca atacttgaaa tgtcggagaa agaaaccgtt
      181 actgttgccg aagaaattaa aacattatct ttttatttag atattgaaaa agcacgtttt
      241 aatgatgatt ttgaatatga aatcattatt aatgaagcca ctttagaaca aataaagata
      301 ccttcattac ttatacagcc ttatgtagaa aatgctatca aacatggttt attacataaa
      361 aacggtgcca aaaaattgac aattgtgttt gaagaaaacg gaaattatct tgatatttct
      421 attatagaca atggcattgg tagaacaaaa agtgaagagt taaaactact tagaaaaaat
      481 ccgcatcaat catttgctac agaagccatg caacaaaaaa ttgatttgtt aaacaaacac
      541 aaaatcaata aaatatccat tgaaattata gacaattaca gcactcaaaa aacaccaaaa
      601 ggaacaactg ttctaattaa aattccatta gatttttatt actcatgaaa gcaattatta
      661 tagacgacga aaagagagct agagtttcac ttcaattact tatggaagaa tattgtccag
      721 aagttacgct tgttgcagaa tgtgaaaacc tgccagaagg tgtaaaagct atccacaaac
      781 ataaaccaga tttagtattg ctcgatattg aaatgcctgg tcatagtggt ttggaattat
      841 tagatttttt taatgaaaac gaaattactt ttgccattat ttttaccact gcctataatg
      901 aatttgctat tcaagctttt aaattatcgg ctttagatta tttattaaag ccaataattc
      961 cagaagaatt aacaaacgca attgcacgat tagaacgcca aaaagctgtt caatttaagg
     1021 ctttggctga aaacattcaa agtgaaaatt ttgaaaaaat tgcagttcct tcgggtaatt
     1081 gtttgatttt attgaaaatt tctgatatta tttacatcaa aggtgaaggc tcttattcag
     1141 aggtttttat gaaaaacaat gtaaaacatc tggtaagtag aaatttgaag aattttgaag
     1201 aacttctatc caatgacaaa caatttctac gaacccacaa atcatatatt gtaaattttg
     1261 atgaagttac aagctataat aaatcagatg gtggctggct tgagttacaa gataacattt
     1321 cagttcctgt ttcttcagaa aaagtaaact taattatgga acgtataaaa attgttaagc
     1381 gttaaattct tcgatctgaa atccaacttg ttttaaatct tcccaaaaag ttgggtatga
     1441 ttttgaaact acttctgcat cttcaattat tagattggtt tttaatgcca atggtgcaaa
     1501 tgccattgcc attctgtgat cttgataggt ttttatggaa atgttatctt taattctagt
     1561 agaaggtgct aatattaaac ctgaatcatt agttagtatc attgctccta atttagttaa
     1621 ttcttttctt aaggcttcta acctatcggt ttctttaatt tttaatgtat gtaatccatt
     1681 taacctgcat ccgagtccta atgcaaaact tgtaacaact attgtttgag caatatcagg
     1741 tgatttagat agatctagta taaccgattg atggtaatag tttgattttt cgagaataat
     1801 ttcattattt ccaaaagaag tttgtactcc aaaattttta taaatttcta ttaaaacact
     1861 atcgccttga agactatttt ctttaaaact ggataattta atttttgttc ttgctggcga
     1921 taaagcaact atagaatacc agtatgaagc agatgaccaa tcggattcga ttgttatgcg
     1981 ttgacggttg atgattgatg gttggtggtt aactttgatt gtgttgtttt caaaagaggt
     2041 tttaactcca atttcattca ataaagctaa agtcatatta atataaggaa cagaagttat
     2101 ttcgccctca agtgtcaatt ccaaaccatt ttcaagtttt ggtgctatta ataaaagtgc
     2161 ggaaatatat tgactactaa catttgcact tagtgaaacc ttatttttta ttaacttctt
     2221 gccgttgatt tttaatggtg ggaaaccttt tttaccttta tagctcacat ccgctccaag
     2281 ttgatttaaa gcatctacta ctattccgat tggtcggtct ttcattcttg aagaacctgt
     2341 taaaattatt tctttgtttt gttgaatggc aaaataagcg gtgagaaatc gcattgctgt
     2401 tcctgcgtga tgaacgtcta ttaggttgta ggttgaaagt tgaaggtttt gtgtaagtgc
     2461 aagaagcatc acttctgaat catctgattt tgagacgttt tctaactcga tatttggata
     2521 caaagcttgt aacaacaata aacggttggt ttctgattta gaaccggtaa tcttgattga
     2581 agattgatga ttaacgattt ttgatttttg aagttctaaa ttcacaatta cgaatgtctt
     2641 aatacttaat actctaaact taatacttct actactttag tttttcatta ttctgatgtc
     2701 tttcgtggtc gcgattggtt ttcaaatcta attttttatc aaaagctgcc tgtaaatcaa
     2761 ttccagtttg attggctaaa cacaaaacta caaaaacaac atcggctaat tcttcaccaa
     2821 gatctttatt tttatcgctt tctttttcgg attgttctcc gtaacgacgg gcaattatcc
     2881 ttgctacttc accaacttct tctgtgagtt gtgccatatt tgttaattcg ttgaagtaac
     2941 gaacgccgtg gttttttatc cagttgtcaa cttcgagttg ggagtttttc aggttcattg
     3001 ttatactttt ttaagcacga ttttctccgt ttgtttggta atcatttctt tataaaatgt
     3061 tttgacaact tcatacatat cagaactgat aattggtgtg ttaatatcaa aagaatacat
     3121 caattgtatt tgattgcctg tatttgaaat actgtattta aaattgccta aatcatcagg
     3181 caaacctaaa gcttttgatg atggcaaaga ctctacagaa taaccttctg gaatctttat
     3241 acttacactg tacttgtctt gatttggata cacaaaatca attggatata atcttgtttc
     3301 ttgtttaaaa ggattttcgg tttgagtaaa atgtagcaaa ggagaaaaat acattttttc
     3361 gccaataatt tctacaaaat tatttgacga aaatgaataa ttctcgatta taggtttaga
     3421 taattcagca ctattttgaa catcatattc gctaatttct aatccttggt gtcttttttc
     3481 aattttttca atcatcgttt cttttgtaac tccattataa gtatttctgt aattataggc
     3541 attataatcg aaatattgat ctctaacctt gcctaaaact tcaccttgag cattaatttc
     3601 tgccattaaa ttaatcattt cttttgaatt aaattttggc ataagatcta tttgagtaga
     3661 tgttccatct tttctaatta atcttccaaa ccagttcaaa tctctaattg gcagaatatt
     3721 tggagttgaa tatttagcag ttgcatctag taaagttact tggttattta tttctacacc
     3781 aacaataaca taatcaaaag cagttcttga aggaaataaa gaaatcccat ttgaacgtgt
     3841 acttattagt acaggatttg cctcaaaacc agcatatcgc aacatagagg taagcattaa
     3901 atttatttca gctacatttc ctgttttttc ttggtatgct tttctaacac cagtattaca
     3961 agtataactg tagtactcat tccagttcat tttagatttt acgaatgtaa aaagtgctat
     4021 caatttttct tctggcatag ttaaacccgc taatatagca tctatatctt tttcaaaata
     4081 acccgtttta tttaattcgt tcccaaaatt atcattgtca tatattgttt ttacaacgcc
     4141 ttcccaagta tttgataaat ctttatagct tagatttttt tgttttactc ctgataattc
     4201 aaaaacgact gatgaaacat aatttctaat attattaaca taagcttcat cttttaaagc
     4261 tggtgcattt tcgacaatat aggttcttcg aagttcatca taactgaatt cttcttgttc
     4321 aaatgaggta cttgttctaa gtccgccgct tctttcttta gaagtagaac gactcgtgat
     4381 tcttttggct ccttcaatta attttggctg caaaaaccct ttaaaaactg gtgtgtatgt
     4441 gtaatattct ggtgtataaa ctctatattc gctatacaaa acaggaatac ttgattgaaa
     4501 aaaccattcg tcaagtgagc ctaaactttc ggatactaaa ttatattcat attcaattat
     4561 tgaaccaact tttacatttg gcattgttat tttttttctt ccccaaaaat cgtttacttt
     4621 ttcatcaaat tcaccatcac ttttaatttt tgatttttca attttattat ttactaaatt
     4681 ataagttaat tcatttttaa aagaaactcg ctcttttgta cttccgccaa tataatatct
     4741 aactgtttta ttagcccaat cgtaaccttc ttttttataa atttttattt tagtttttac
     4801 atttgtattt agcacaaatc ccttgccttc aataaattca aagtgaactt ctccaataga
     4861 aaacaagatt gctgccacag cacttgaatc tgttggacaa attttctctt caagttcttg
     4921 aatggttact tttcctaact ctctattttg agctatcata tttataccaa taaataataa
     4981 taacaataat gattttttca taattgatta agtttttgaa atgactactt ttgaattgtc
     5041 tgttttggca attgtttctc taaattttct ataattttca aatttagaac tttcataaaa
     5101 tcctttatta ataattaatt ttcgtttgca aataatgttt ttgttatttg aaatgataaa
     5161 atcaatttta tagcttccaa attctgtttt aagctctgct ccatttggct ttgcttccac
     5221 aacgtatcca tatggcaatg ttactgttat ttcttcttca tcagtataac cactttcaat
     5281 ctcaaaagga agctcacgtg tacgatgttt ttttggcaca taactagatt gatcaaaagc
     5341 attgattgca aacattaatt ttccatttac attttgggca taatttttag cttcaagttc
     5401 aaattcttct gtgaatttta ttttattttt atcatttata aaattaattt tcgaaaagga
     5461 aatattgtta atattactat attcttcttt gtaattttta atttgatctt ctttactcag
     5521 tcgttctttt tgaaattgat tatcatactg aagtccaaaa gattctattt ttaaagttgc
     5581 cgtaattttc ccatcatcag aaattttata agatcctttt atttgtttta aattatcatt
     5641 ttcatcaaaa gttttagttc taactatttc tcctccgtct ggtttaatta aaaaagcatt
     5701 tctatttcca gtaaatccac cctgaaaacc aaacggttgt atttgacttg tacactctaa
     5761 ccacaagtaa tcatttttgt ttggaattgc taaaatgaca tgattacttt gaaaagaaac
     5821 taaatctgaa taaatttctt tattagtatt ttgaccataa agcctgctat aaaatgattg
     5881 aacgcctact tcattcagta atgctctggt ataatttgtt aacgctttac agtcaccata
     5941 acctaactta tctacatctt ttgcattcat tggtttccaa ccaccaattc cttcttgaat
     6001 actaacatat ctagttttat cttgaacata cttatatata atttcagctt tctttaatgg
     6061 atctgtttca ttcgcgacta aatttttcac tttaacctta gtttcttctg gtaattcagt
     6121 agtaccattt aatatattgt cataaaacca tttacccatt tcattccagg aatttgctac
     6181 tccattaata ccttctaagt gaaatttatc taacccaaaa ataaccaatg gaaacacttt
     6241 actaaataca ggagctaatt cttctttttt acttgcttga acgttagccg aataaaaatt
     6301 aatttgtccg tttgttgatg ttttattgat taaatatttt tttgaaaaat tcaattcctt
     6361 aaaattgact ttcaattcag gcttaaaact tatagttagt tttgtgtttt cggtacttac
     6421 ataataatca tctaccggac tccaagaagg aataaaagct gtatttgaag tttctatttg
     6481 actttcataa accatcgtaa aaggataaac agttggcgta taatcaagat ataaaacacg
     6541 attgtcatta aaaattgaaa tcccgtcagc tacacttaca tctttaaagt cttttttcct
     6601 aaaagattta agttcttttc caaaggcatc atatgcaatt gcttcaattt tagaaacttt
     6661 tctattctta tcataatttt cagaaagatt aaggtttctt aaaccaaatt catttaacac
     6721 agtagtaacc actttagtag taatgatcat tgcattttga gatgaaatag ttatagtaat
     6781 atcgcttaat cgaatgactg cattggcatt attttttaaa ctgtcagaaa tagtagtgac
     6841 agtatattca gatttttgag caaaaaacaa attgctaaag aataatgaaa acagaataat
     6901 tatagttgat ttcatctaaa tttaatttta tcaaatataa aaaatttagt ttttattaat
     6961 acaattattc tttgtttttt gtgtcaataa ttatagttac tggaccatca tttgtgagtg
     7021 ctactttcat atccgctcca aaccgtcctg tttggatttt tttacccatt tcagtttcca
     7081 tttgaataat gaatttttca tataagggta ttgcaatttc gggttttgca gctttgatgt
     7141 aacttggtct gtttcctttt ttggtggaag catgtagtgt gaattgagaa actacaatta
     7201 tatcgccatt tatatctttt acagaacaat tcattacctg attttcatcg ctaaatatgc
     7261 gaagattggc tatttttgag cacaaccaat tgacatcttc atgattatct aattcttcta
     7321 tacctactaa aattaataac ccattttgga tttcagctac aatttttgat tcaatcgtta
     7381 ctgatgctga agttgttctt tgaataacgg ctttcaattg aataaaatta tttagttaat
     7441 ttgtgtccta tttttataaa caatgtagat atcactgtga aaagcaccaa aagtattatg
     7501 ctatttgtca taactttagt taatcctatt tcagctacat tcacaaaagg atatgggtaa
     7561 aagcctgaaa aaaaccctct tcctaaaata taaaacaaat atactaatgg ataaattaac
     7621 catagtaata cacttttata cataatagca cttttatttt cataaaaata ccaaaataaa
     7681 atcactaata aaggtattat ggaatgcaac aattcgtcaa ctaatttttg taaccctttt
     7741 ggttcccata catttcttag agcaaattgg tacacaagac ctacaattgt aatataaact
     7801 gttattgaac ttaaatttcc aggtttattt ataaaaggaa taatactttt ctttatatta
     7861 ataaaaagca aagtaaaata tattccaaca aaaagatttg ttagtattgt aaaaaaacta
     7921 aaaaagcgaa ttactgtttc tgttattgat gcaactctgt tttcaatcat taaataaaat
     7981 tgaatagcga cagtaattaa ggcaagaatt ccaatactaa tagatgctac ttttttcatg
     8041 ttttatagat taaaaaatta cttcctcgtt tcatcacttg cttttctttc ttcatcgtag
     8101 ttatctgttc ggtaatgttc ttcatcacct tcaagaattt ttagataact gttgtagcgc
     8161 gaccaagcaa ttttgttttc ttctaaagct ttttttacag cacaatttgg ctcttcttta
     8221 tgcaaacaat tgttgaattt gcattgttct tttaaaacaa aaaattcagg aaaataatca
     8281 ctgatttctg ttctttccat atctacaatt ccaaagcctt ttattcctgg tgtgtcaatg
     8341 attttggcat caaaagacaa atcatacatt tctgcaaaag ttgtagtatg ttgtccttgt
     8401 ttactttgct ctgaaatatc ttttgttttt aaatttaaat tgggttnnnn nnnnnngcat
     8461 ttaccaaagt agattttcca acaccagaat gaccagaaaa catagaaact ttgcctatca
     8521 tttcgttttt taatgcatct attccaattg tttctttcgc agaaactttt aaacatttgt
     8581 accctatttg agaataaaca tattgcaaaa aaagctgttc gtcttccatt gcatcatcgt
     8641 aagtgtctat tttattaaaa actaaaatag cttctattcc ataagcttct gccgttacta
     8701 gaaaccggtc aataaaactt gtagttgttg gtggattatt aatggttatt agcagaaaaa
     8761 caacatcaat atttgaggca attatatggg tttgtttgga taaattaacc gattttcgaa
     8821 caatataatt ttttcgttcc tgaatgttat gaatctgacc aatggtttcg tcggtagatt
     8881 tgtcaagttc aaaatctaca atgtcaccta ctgcaattgg gttagtgctt ttgattcctt
     8941 tcattctgaa cttacctttt atacgacatt caaaaacttg attatcatta gtttttacag
     9001 aataggaact tccagttgat ttataaacga ttccagtcat agggcaaaga tactgttttg
     9061 attgaagaaa ttgtaaataa aaaaagttgc tatttctagc aactttcagt ataaattatg
     9121 tcattattag tatttgtaag caactccaat cattcttgtt tgagaacttc ctaatccttt
     9181 ttggtatgtt tttccaactg cataatcttt gtcttctgtt accaaaacta caacacctcc
     9241 aagtttatag caatcttctt ttaatctttc ttctgatttt ttatcagcat ttgctccagt
     9301 tctgtaactg atgaaagctg tttttccttt aacctctcca attcttgtca aaccttcaac
     9361 atcctctggg tttttggtta agattacttt ttgaaaagca tcttttcctt caagaacaag
     9421 tttttcagta actacttgtt ttcttccaga tgcataagtg atactgttaa ttgcaatttt
     9481 tgaaattttt gtagatacat cttcaccttt gtatgtaaag gttatagttg caccatcagt
     9541 actacttact tttccttcaa gagtactacc gttgtgaagc gttacaacgt cttgtgcttt
     9601 agctaagttt aatccaaata caagcgttaa tactaaaata atttttttca tgtttttact
     9661 gttttaaaat attaattcag ctgcaatatt aaataaaaat ttgctttagt ttatatacga
     9721 caaattacgt attttactaa ttgttaatta ttttttcctg atgactaata ctttcttggt
     9781 gaatggcttt gaataatttc aatataaatt cttcactaag gcctttgttt tcgccttctg
     9841 aaatcatttt attcaacacc tcattccatc ttttattttg caaaatagct acgttttttt
     9901 ctttttttaa tagacctatt tcttctgcaa tcatcattct ttttcctaaa atttctacta
     9961 ttttggtatc aaaaacatct atttgagtcc tttttttatt catttcttta gtgaaatcat
    10021 ctgcatcatc agatactttt cgaactctta actctttgat aatttgtttt aaagtggctg
    10081 gtgtaacttg ctgtgctgca tcactccatg cattatctgg atcaatatgg gtttctatca
    10141 tcaatccgtc ataattcaaa tctaaagctt gctgcgacac ttcttgaatc aaatttcgtt
    10201 ttccagtaat atgcgatggg tcgcaaagta aaggcaacac tggaaatcta ctttgtaaat
    10261 caatcgcaat ttgccattca gggttgtttc tgtattttgt tttttcatag gtagaaaacc
    10321 ctctatgaat ggcgccaatt tttttgatac cagcattttc tagtcgctct aatgcgccaa
    10381 tccaaagtgc taaatcagga tttacaggat tttttactaa tacaatttta tctgttccat
    10441 ttagagcatc tgcaatttct tgaaccgcaa aaggatttac cgttgttcga gcaccgatcc
    10501 acaaaacatc aatatcatgt tctatagcca atttgacatg attggcattg gctacttcaa
    10561 ttgccattag taatcccgtt tgagctttgg ctttttgcaa ccactttaac ccaatagctc
    10621 caacaccttc aaaaccacct ggtcgtgttc ttggtttcca tattcctgct ctaaatattg
    10681 aaacatcaga atcttttaac tcgtgtgcaa tttttaaaac ttgctcttcg gtttctgcac
    10741 tacatggtcc agctattact aatggatgcg tcaatttaaa atcatctaac cagtttgtat
    10801 gttgttcttc ctttttcatt aattatattt gatacaaatt tataataatc tcattacttt
    10861 tgttccaaat aaatctacta tgtcgataga agttcaaaat atttcaaaaa gttatggtgc
    10921 tcaaaaagca ttagaccaaa tttcattttc tgtaaaaaaa ggcgaaatcg ttggttttct
    10981 aggtccaaat ggtgccggaa aatcgacttt aatgaaaatt ttaaccactt ttattgctgc
    11041 cgatgaagga aaggcttctg ttaatggaaa tgacgttgta gaaaattcaa aatcggtgca
    11101 acaatcggtt ggttatttac ccgaacacaa tccgttatat ttagacttat acgttcgtga
    11161 atatttggct tttaatgccg atgtttataa aatctcgaaa tctcgaatcg aagaagtaat
    11221 cgaacttact ggtttaacgc ctgaaagtca caaaaaaatt ggtcaattat ctaaaggata
    11281 tcgtcagcgt gttggattag ctaccgcttt gcttcataat cctgaagtgt taattttaga
    11341 cgaaccaacc acgggtttag acccaaatca gttggtagaa attagaaatg tgattaaaaa
    11401 cgttggaaaa gacaaaacgg ttttcctctc tacacacatc atgcaagaag tggaagccat
    11461 ttgcgaccgt gtaatcatca tcgataaagg aaaaattgtt accgataaaa agctaaccaa
    11521 attagtttct gacgaagccg agcaaatcat cgaagtagaa tttgacaaac ctatttctga
    11581 aagcattttg aatactttac ccaacttaaa aagtgtaaaa aatcctcaaa acaacatctg
    11641 ggaattaact tttacaacca atgatgatat gcgttcaata gtatttgatt ttgccaaaga
    11701 taacgacttg aaaacacttc aaatttcgtc aagaagcaaa aacttagagc agatttttag
    11761 agagaaaacc aagaagtaat taaggataaa ccactcttcc ttgatacaat tcttgaacat
    11821 tcactattgg tggtgtagtc gtcaaaatca aatctccagt actattcaaa acgccttcta
    11881 tactttgaac tggcgttaca tacatatcgt tcgaaccgcg atgaaaaaca ttaatttgtt
    11941 gtgcaatcaa gttttgtgct tcaatgcgcc cgtctccata ataaaaattt agactcgcca
    12001 agttagtagt tcctgaaaga taaaatcgcg acacattgtt gttagaaata gtcaaggagt
    12061 tgtttgcaac ttcaacataa aaatcgcccg taccagctcc attttcgcca tcagcatctt
    12121 tatctaaaga aagaatactc aaatctggaa aagtaagcac accattcgaa cgaatatctc
    12181 tatccgtttt cgaataaact tcggtcaacg ttggcgtagt aatataaact ttagtctgtc
    12241 cataatctct aacccaattg caactcgttt gatctttaaa aacaagttgg tcgccatttt
    12301 gtgttacgct aatatcgttt ataaaattct ccccggcctc aatttcgacc ttataattcg
    12361 gtccttgggt aataattata gcaatgccac gatacacttt aattttgcta aaagcagaaa
    12421 ccgcaatttc tttaaccacg gtagcacccg aactttcaat acaatcactc ggtttttcac
    12481 acgaatttaa aagtagtatt accaacacta aaagaagact ctttttcata accatagttt
    12541 taaattctat acccaattcc aaattccaaa gcttccgcca aaaataaatg tgtttttata
    12601 gaaaatccac caaaaatctt atccgtaata cgatatttca tgcccactct gtcataaacc
    12661 ggaatgtctt tcttaaacgg ttgatacaca taaacaccca actgcgcctc taacgaaagc
    12721 ctattaatca ttaattcatg accaataaaa actccaacac gtttataatc agtatctgcg
    12781 tcaatatttt tatccggata cgccaccgaa taataataaa tataatctct aattgattcc
    12841 gtcaaaaaca actccgttcc cagttgcaac gtcgattttc tattcaaacg cttatcaaca
    12901 taaaaaccaa tatgataaaa aggatgctga ccactcctaa taatcggact ttcatttacc
    12961 ccagttctaa aaaccacatt ataatgaagc ggttcgcgat acgatttttt aatcgaactt
    13021 gtatctactt cacgaataac aacactttca aaatcatagt tcacaccaac atttaacaaa
    13081 taggtgttaa taccactatt cggcgactta aaccgaccat ttgaataatg cgtaaacaaa
    13141 atgcctgcat gaaaccctat atttttaacc aaattttgat gatcatacgc aaaaccaaaa
    13201 ttagtattac ccataaactt agtcccaaaa gctttattct tactattcgc tactttatca
    13261 tacggctcag ttgccaaagc aattccttgc ccaatactca actgcaacct ccgcttccaa
    13321 aaataaaaat tatacaaccc accaattgca taattctttc ccaaaggcgc actatgaaaa
    13381 tcttgataca aaaaataagc cccataatcg ggataattat aggcctgatg ccactcctta
    13441 gaaccatctg ttcttttcaa aaaacgcacc atcaaaccat ccggatgacc attcaccaac
    13501 tgatacatat cctcagtatg tggcaaaata tttcctcgca acactgatgc ttcgacagca
    13561 aagttattgt tttgagaaaa acacaatgaa gaaaatagac atgaaactaa aaaaaagaaa
    13621 gacctcatcc gaaaaattta agcaacgcaa atataaaaga atatttcaat gtcagttatt
    13681 tgaggtcgaa tggtttttga aaatattata tgtttaaaat tttggcaatt gaattttaaa
    13741 aaatctcttt agcaatagct ttaatattct cggcttttcc cattgaataa taatgcaaac
    13801 atggaattcc tgctgctaag agttctttgc tttgctgcaa acaccaatca attccaattt
    13861 gttttacggc atcattatct tttgctttta cgacttccat tactaaatcg tcaggcaaat
    13921 cgactttaaa ccgatgtgga attaggttta actgcttttt ggttgctatt ggttttaacc
    13981 ccggaattat tggcactgtt atacctgcca atctacattt ttctacaaaa gcaaaaaact
    14041 ttttgttatc gaagaacatt tgagttacaa tatagtgagc accgtttttt atcttttgct
    14101 ttaagaaatg aatgtcgcta tcgatgcttg gtgcttccat gtgtttttcg ggatagcctg
    14161 ctattcctat gcaaaaattg gtttcggcag agttttttaa gtcttcgtct aagtaaattc
    14221 cgttgtttaa gtttgtaatt tgcaatacta ggtcggaggc aaattcgttt ccttcttttt
    14281 cgggtttgaa gtaggtttcg tttttcattg catctcctcg caacgccacg acattgtcaa
    14341 ttcctaaaaa atctaaatcg attaataaat tctcagtatc ttcttttgta aaaccaccac
    14401 acaaaatatg tggaatggca tcaacttcat atttgttttg aattccggca caaatcccaa
    14461 cagttccagg tcgttttttt acaattttct tttgcaaaag tccgcttggt aattctttga
    14521 attcgtattc ctcgcgatga taggttacat caataaatgg cggtttaaac tccattaacg
    14581 ggtcgattcc gtcaaaaata gactggatgt tttgtccttt taatggtggt aatatttcga
    14641 aggaaaatag tggtttccca tttgcgtttt ctatatgttg ggttactttc atagtttttt
    14701 gttgtcggtt ttttgttgtc ggttatcagt ttacatgttc aactgacaac tgataactgt
    14761 ttttactctg ctatattcgg tgaaagccat tttgtagcaa cttctgttga aattcctctg
    14821 cgtttggcgt aatctattac ttgatcttct ttaatttttc ctagcccaaa atacttactt
    14881 tctggatttg caaaataata tcctgaaact gatgatgctg gccacattgc catactttcg
    14941 gttaatttta ctcctatttc ttgttctacg tttaataatt tccaaatcgt tggcttctct
    15001 aaatggtctg gacaagcagg atatcctggt gctggacgaa ttcctttgta tgcttctttt
    15061 attaattctt ggttattcaa attttcatca gaagcataac cccaaatttc tttacgcact
    15121 tttaaatgta aatattctgc aaaagcttcg gctaatctgt cacctaatgc ttttatcata
    15181 atcgaattgt aatcgtctaa atcttttaca tattgattgg caatttcgtc taatccaaaa
    15241 cctgttgtaa cacaaaaaca acccatgtaa tcttgttttc ctgaattttt tggagcaatg
    15301 taatcagcta acgctttgtt tggtgcgcca gctgtttttt gtgattgctg tcttaaagtt
    15361 agaaaagttg taggttgtag gttgtaagtt gtaggttgga tgagaatgtc gtcatcgttt
    15421 acggaatttg ctggaaatat tcctaaaatt ccttttgcgg taagccattt ttctgaaacg
    15481 atttggttta gcattttttg tgcatcggta aataaattag aggcatgttc acctactatt
    15541 tcgtcagtta aaatcgctgg atattttcca aacaattccc atgattgaaa aaatggtgtc
    15601 caatctataa aatcaactaa ttgggatagt tcaacatcaa taatttttgt tccaatgaaa
    15661 ttaggtttta caggtgtaaa attatcccaa tcaatttgaa ttttattttt tcgtgcttgt
    15721 tcaatcgtta agtagttttt atctctactc ctatttaaat atccttcacg aagtttgtca
    15781 taatcttcac gaatggtttt tacataattt tctttggttt cggattgcaa taaattacta
    15841 gcaactgtta cggcgcgcga agcatcgttt acatgaacaa ctgtttcttt atattgagga
    15901 gcaattttca cggctgtgtg cgcacgagaa gttgttgcac caccaatcat tatcggaatg
    15961 gaaatgttta atttatccat ttctttggcc aaataaacca tttcgtctaa tgatggcgta
    16021 attaatccgc ttaatccaat aatatctaca ttgttttcaa tagctgcagc tatgattttt
    16081 tctggtggaa ccataacacc taaatcaatg atttcaaaat tattacaagc caatacaacc
    16141 gcaacaatat tcttaccaat atcatgcaca tcacctttta cagttgccat caacactttt
    16201 cctgcagatg acgattttcc agttttttga gcttcaatga atggcaataa ataagccacc
    16261 gcttttttca taacacgagc agattttact acttgtggca aaaacatttt cccacttcca
    16321 aacaaatcgc caaccacatt cattccttcc atcaaattga tttcgataac ttcaattgct
    16381 ttttcagaat ttgctcttgc ttcttcaaca tctagttcta taaattcatc tattcctttt
    16441 actaatgaat gggttaatcg ttcttggatt gaaccatttc tccattcggc aatttctttc
    16501 tcattcgatt taaaatcgcc tttaaaactt tcggctaaat ctaacaatcg ctctgttgca
    16561 tcttctcttc tatttagcaa aacatcttcc acatgttcta ataaattttt atcaatttca
    16621 tcgtaaatct cgagcatttc agggtttaca attcccattg acattccatt tttgatagca
    16681 tggaataaaa acgccgaatg catggcttca cgaactttat catttcctct aaacgaaaat
    16741 gaaacattac ttacgccacc agaaacatgt gcataaggta agttttcacg aatccatttg
    16801 gttgctctaa aaaaatctaa ggcgtttaat ttatgttcat ccattccagt tgccactgga
    16861 aaaatatttg ggtcaaaaat gatgtcttct gctggaaaat gaacttcatc aactagaatg
    16921 tcataacttc ttttacaaat ttctattctt ctttcgtaat tatcagcttg accgttttca
    16981 tcaaatgcca taacgataac tgctgcacca tatcttttta tcaatttagc atgatgcacg
    17041 aaggcttctt ttccttcttt taaagaaata gaatttacaa ctgcttttcc ttgcacgact
    17101 ttcaatccag cttcaataat ttcccatttg gaactgtcaa tcattattgg aacacgagca
    17161 atatcgggtt cggcagcaat aagatttaga aactttgtca tggcataaac gccgtctaac
    17221 attccttcgt ccatattgat gtcgataatt tgtgctccgc catcaacttg atctcgagca
    17281 acagaaagtg cttcgtcgta tttctcttct ttgattaatc gaagaaattt tcgtgaacct
    17341 gtaacattgg ttcgctcacc aacattcaca aaattggttt caggagttat tactaatggt
    17401 tcgagacctg aaagttttaa atattttgtt tctgacattt tatgtttttt tgtcacaaag
    17461 gcgctaaggc acaaagtttt atttttttta aaccaataag aacattaata ttcattaagg
    17521 tttttgcttt tatttcgcta atcctgcaag gtttttaaaa ccttgtaggt ttgttttatt
    17581 tttttaaacc attaagaaaa ttaaggttta tgttttttaa ttttatacct acaaggtttt
    17641 ggaaatctta caggaaatct tatgctaatt ctagtgttct tggtttgaat tctttgacaa
    17701 catcagctat tgctttaata tgttctggag ttgtgccaca acatccgcca ataatgttta
    17761 ttaaattgtc ttgcaaatag tcgcgaatta aggcttgcat ttcaagtggt gtttgatcat
    17821 attctccaaa agcatttggc aaacccgcat ttggatgtgc agaaatattc aattgtgtgt
    17881 tgtgagctaa tcgttttaaa tacggtttca attggtcggc acctaaagca caattaaatc
    17941 caattgacaa caatggaata tgtgaaattg aaattaagaa cgcttccacc gtttgtcctg
    18001 ataacgttct tcctgaagca tccgtaattg ttcctgaaac cattaccgga atatcaatat
    18061 ttcgttcttc ttttacttct tcaatagcaa atagtgccgc tttggcgttt aatgtgtcaa
    18121 aaatggtttc caccaataaa acatcacatc cgccgtcaat caacgcttca acttgttgtc
    18181 tgtaagcaat tcgtaaatca tcaaaagtaa ccgcacgata tccaggatca ttaacatctg
    18241 gcgacataga agctgttctg tttgttggtc caatggaacc agctacaaaa cgtggtttat
    18301 ctgtaaattc atctgcaact tctcgagcaa ttttagctga ttgatagttc aactcataga
    18361 ccaattcttc catatgataa tcggccatac caatggttgt tcccgaaaaa gtattggttt
    18421 caacaatatc agcaccagct tgaaaataca aacgatgtac ttgctttaca gcttcaggtt
    18481 gtgtaatgga aagcaaatca ttattccctt ttaatggatg tggaaaatct ttgaatcttt
    18541 cgcctctaaa atcctcttct gagaaattat tgcgttgcag catagttccc attgcaccgt
    18601 cgagtacgag gattctttct tgaatagctt cttgaatttt tgacattttt ctaataacgt
    18661 tttataattt aattgttatc agaaactgga aagtatttct ttgggttatc tgtttcgcca
    18721 aggcgaatag aatgtagcac cttctacaag tgtagggttg ctaagctttc atagagtcta
    18781 atctctccag ctttcgtgat aacaatcatt atgtttatga acgatgcaaa gtaaagacat
    18841 agaaaattga aaaacaaaag aaaaaaagaa aaaatcaatt atatcgattt cgtaattgca
    18901 attaaaaaaa tattgaaatt gtttttttct attcaattag tctttataca tttgcaaacg
    18961 aaaacaaaga ggaaaaattt gtactgattg caacctcgtt aaaaaatgct aaagcagaac
    19021 tctcctttag ctttttgcaa tctttacttt ttattgaata atattaaact aaacaatagt
    19081 tatggcttat ttatttacgt ctgagtcagt tagtgaagga catccagaca aagttgcaga
    19141 tcaaatttct gacgcattaa ttgataattt tttagcattt gacccagagt cgaaagtagc
    19201 ttgtgaaact ttagttacta ctggtcaagt aattttagct ggtgaagtaa aatcaactac
    19261 ttatcttgat gttcaacaaa ttgctagaga agttattcgc aaaattggtt atacaaaaag
    19321 cgaatacatg tttgaagcag attcttgtgg cgtactttct gcaattcatg aacaatcttc
    19381 agacatcaat caaggagttg acagagcaag caaagaagaa caaggcgcag gtgatcaagg
    19441 aatgatgttt ggttatgcaa ctaatgaaac tgaaaattac atgccattgg ctttggattt
    19501 gtctcatgct ttgttaatcg aattggctaa cttaagaaga gaaaataacg aaattaaata
    19561 ccttcgccct gatgctaaat cgcaagttac tttagagtac tctgacgaca acaaaccaca
    19621 gcgtattgat gcaattgtta tttcaacaca gcatgatgat tttgctcctg aagctgaaat
    19681 gttagctaaa attaaaagtg atttagtttc gattttaatt ccaagaatca aagcaaaata
    19741 tcctcaatat gctcatttat ttaatgatga cattacgtat cacattaacc caacaggtaa
    19801 atttgtaatt ggtggaccac atggtgatac tgggttaact ggaagaaaaa ttattgttga
    19861 tacttatgga ggaaaaggtg ctcatggtgg cggtgcattt tctggaaaag acccaagtaa
    19921 agtagataga agtgctgctt atgctacaag acatattgct aaaaacttag ttgcagctgg
    19981 tttgtgtgac gaagttttgg ttcaagtttc ttatgcaatt ggagttgcaa aacctacttc
    20041 tatcaacgtt gtgacctatg gcacttcaaa agtaaaccta actgatggtg aaattagcaa
    20101 aaaagtagaa gctatttttg atatgcgtcc ttattttatc gaacaaagac taaaattgag
    20161 aaatcctatt tatagcgaaa ctgcggctta tggacatatg ggaagaactc ccgaaacggt
    20221 aacaaaaacg tttcaattgc caaatggcgc accaaaaaca gttacagttg aactgtttac
    20281 ttgggaaaaa ttagattatg tagatcaagt aaaagctgca ttcaatattt aattaataaa
    20341 atcaaaaaca tttaaaacct cttaattcac ttaagaggtt ttttttatta ctttgtacaa
    20401 aaaaaattga ttgaattact aaatagaata gaaaatcgac taaaatttag acctagttac
    20461 tatttacttt ttgcgatttt gaatggtgcc gttttgatga attttcacaa ctatcttttc
    20521 aaacactcaa ttggtattgt ttttcttttt tgttttgtta tttatactat ttataaacct
    20581 aaaaattgga tatctgttat aggaagttgt ctattttcgg tgtactactt attagatttt
    20641 tttccaagaa ttggcaatca ttctagtatc attttattta cttctttaac cattttactt
    20701 ttatttatat tcaaattatt cagcaaaaaa gaagtattaa attcaaaact tctggcgtat
    20761 ttctttagaa tatttacggt aaccgtttat ttttatacag gatttcataa gctgaatacc
    20821 gattatttta atacttgtgt aagttgtgtt aatgaaataa atgaatatac actttccaat
    20881 atttttggtt ttccaataaa gattggagat tccctttcta gattctttca aattattgct
    20941 ttaatagtag aatgtatagt tccattcgga ataatgcact acaaaacccg tagctattcg
    21001 gtgctacttt tattgttgtt tcatagctat ttatcattat ctgtatttgc agattttagt
    21061 gccgttgggt tgtttttatt actaggctgt tttattgatt ttgaagaaaa ttctttttca
    21121 aaaaaaacta tattttatat tcgactatat ttaataatga taatgatttc tgtaatcagt
    21181 ccgatatttt taagcagaat ctcagttcaa acttctgtat ttccttttat aaaaggtttt
    21241 attttcttag ttggttatct ttcatttttt atttatttct ttcgaaatca tgatgccaaa
    21301 cgatattctt ttcggcgaag ctattttatg ccattattca tcctttgcat atcaataagt
    21361 ttttggacgt taaaaggtta tataggatta ggaaatgcag gaaacctaac catgtttagc
    21421 aacttagtaa cagagaaatc gatgaacaat catttgttaa ttgatactaa aaaaacaaaa
    21481 cttttcaact ttgaagaaga caacgtatat atcatcaaaa tggataatcc taatataaag
    21541 gaaaattaca ctggctacaa attacctatc gtagaattta gatttcttgt aaactattgg
    21601 tcgaaaaatt ttgacaagcc aattccttgt atattagttt ataaaaataa aaaatacacg
    21661 ttcaaagata taaaaacctc tgaatttaaa aactcaaaat ggtggtacaa atacttaaat
    21721 ttccgaaaga ttcaaactac ttcgccaaac gaatgccgct ggtaaattag agcctattac
    21781 tttttatttt ctataaatta tacttcaaag tgaaaattaa gtatcgtggt tgaatcgtat
    21841 attgtgaatc actcgttgaa aactgcgaga aactattatc cttcaatgct tttgtgttta
    21901 acaagtttgt cacacttact ttatactcaa agtgactgtc ttttgttttt tggaaaatca
    21961 aacttgctga caagaaatcg tattcatttt caaccgattt gtctttatta tagtaatgaa
    22021 agtattcgta ttcactcaca aacgaaaaac tcttcaggaa aaagtattct aacttcgcat
    22081 ttggtctatc ggtataaaac gtatcatttg gatattgatt tacagaataa ccataaccta
    22141 attctaggtt tggaaagttc ttgaagttgg ttgaaatatc aaagtcatag gtttgcgaaa
    22201 aactttcatt cgttaataag acatcttcta atgttattct atccacttga atgttattgt
    22261 attttgacca gttcaaattg gcgctaattg aggctttgta atacttaaag aaagaacgac
    22321 cgtaacgtaa atttcctgaa agactttcat ctgcaaaggc tgaattgatt ggcgaagaca
    22381 cttgattcac attgttaaat aaagctctgt ttttgacagc gtcaaccgtt cgattataat
    22441 taataaatgc cgaaatattt tcgaaattaa acatgttgta cttaaaataa cgcaacgaat
    22501 gcgactgttg tatggcattc tctaacgttc tgtttccgct ggttaaacta ttgtaattat
    22561 taaacacata tccttcggct aagcggttaa tatctgtaaa gttattattg aaagagaaat
    22621 tataggttaa cgattctgcc ttcttaattt gataaattgc caagaaatct ggaagtattc
    22681 taccaaaatt ttgcttatag ctgcttccca attgggtatt gttgagcgaa tagttatgaa
    22741 tactaaatcc tggtgttaaa gtaaacttgc ctgttagtaa tttataatgc aaaccaagaa
    22801 aagcatcatt aaacctgtag tttacatcgt ttgtattttc agaatcatcc aaattgttga
    22861 tgtcttggtt atctaatatt tgaaatattc ttgagttgaa gttttgatac gaatatgtat
    22921 ttccgaaggt tacattgatg ttgctctttg gtgtaaccat atagtaataa tctagctttg
    22981 tatcaatctt attggtcttc acaaaacggt cttggttgat gtcgtttctg ttttgtccgg
    23041 caatgtatcc ggataaatta aacggttgcg tttgtaaatt ggcgttgtaa aaaggatctt
    23101 cttcttgata cagatgttgc atctctaatg caaaaacatg tttagaagtt ggtgtgtaat
    23161 aaaaactcaa gttctgattt attgtaacag ggttttgggt tttattgctc aaaatagtct
    23221 ctgttgttga aatattgtct tcatcaatca cttctcttag taatgaattg tcttcatctt
    23281 gcttagaaaa cttgctcaac acatcataat ccatctggaa attagcattt ggtttgtaga
    23341 gcgaacttaa cttcatcaac cctaatctac tattctgatg cgctatttga tttctatttt
    23401 cggtagaagc tacttcggtt gaatttggtt ttaaataatt agtgttagtc actgtttcaa
    23461 tagctgtaac cgaagatgaa aggattccaa aaccacttaa cgtccatgtt tttgaaggat
    23521 tataggagaa gttggtagca ccaaacttgg tgtcaatttc tttggctcta ttgtttctta
    23581 ataacgatac gcccaaatca ttgctcaaca cattaaagct agaaccacct tttgacatca
    23641 tacttctaaa tccgccagtg aatttaaaat aatcctgaat ggttagcggt aattctccaa
    23701 tgttattgaa gtttgtaatt acattaatgc tatattttgg gttataataa aagagcttcg
    23761 ggttgattat atatcggtca aacttgtttt tatcgtccat tccgccacca gcagtaacat
    23821 cgccaaacca aaagtttttc tttccatctt ttaacttgat gttcatagca agattatctt
    23881 ggttgttttc aactcctttt aggatggagt tttcattaaa gtttctcaat acctgtacct
    23941 tgtctatggc gtcagcagga atgtttttaa cgcctagttt tgtatcgccg tcaaaaaaat
    24001 ctttgccttc taccattaat ttggtcactt tctttccttc tacttctact tcgccatcgg
    24061 catttacttc tacgccaggc aactttttaa gcacgtcttc cagttttctt tcggtgccgt
    24121 ttttaaacga atcggcatta taaactattg tatcaccttt tatagaaact ggcatttcgc
    24181 gtacaatttc tacaccttca aggtctatac caccactttc catagtgatg ttttgagtaa
    24241 tgttttctga ttctgtagtt atggttaact ctttattttg catcccaaga taactcagct
    24301 taaccgtata ggttgtattg ggtttcaagt tcaaaacaaa ccttccttta tcgtttgtta
    24361 tagcatacga atccattcct tttgtggctt gattcatagc cataatgttt gccatttcta
    24421 gtggctgctt gttagtatct tggatgattc cttcaaaacg aacgttttga gaaaaactta
    24481 aaagtgaagt aatcgttaaa ataaacaata gtaatttttt cattcttttt gtggcaaata
    24541 atattaatgt tctatagttt ggaaaataac attcccatct tcatctttca tgctgtccat
    24601 tttcttcttt tgtatctcat caaatctctt ttgtgacact ttttctccgt tttttggttt
    24661 cttgatttca gatttgtctt tagagtttaa gacaatcttt gaacataaaa taatgttgtt
    24721 ctcttcgttt acctctagta tcaatccggg taaaccccaa tagttgtttg gtccaaagct
    24781 tactggaatt tctggagtgt accaagcaac aattactttg tctttgggtt cgtccatctt
    24841 aaataatgcg ggctttactt tttccttttt caaaaactct tggtaggctt ctttctcatt
    24901 ctcggtaacc ggaatgatta actcagcttt aaagcagttg taatcaccaa tcttttttga
    24961 ttcattcaca agtttccaat tggctttttc taatggttct gatatcaaaa actctttgcc
    25021 aaaaatttca tcttcggcta aggttgtctt gtctttaata ttgatgtatt tttttccagc
    25081 tcccgagaac tttatcgaaa tgctcattcc gttgcttggt gcttggggtt gttgtagttg
    25141 ttctacttct tcataaaccg cttcgtactt gttgaaggtt aacgaaaatg ttttttgaga
    25201 cgacttgttc aacgcttctt ttaaagcagc cgttaaatcg gggttctcat ctactttatc
    25261 cgatttaata ttatcctttt tgaagatgta tttagacatg tattctgctt tcccctgaaa
    25321 atcttgtgcg tttgaaagca acgttcctaa caataaaaat ggtagtacta tttttttcat
    25381 aaagttgttt tatcttggtc caaagccacc tcgtcctctt cctccaccca tttctctcat
    25441 ttcttccatc ttgctgatga ctgtttcgtc atattctttt tgagaaacta ctttaccatt
    25501 tttaggcgct tttatttctg ctttttcttt agcattcaat acaatttttg aacaaagcgt
    25561 tactgttttt ccgtcattaa tttctaatat taatcctggt aaaccccaat atccttctgg
    25621 tccttggctc accggaattt ctggagtgta ccacgcggta atggtgattt cttttggcaa
    25681 atctatttca tctaagaagc ttgttttttt ctctttttcg tcttcttttt tagcatccgc
    25741 agctttttcg tctttctttt cgtcttcttt ttttggtcta aagtttctaa aatctgtttt
    25801 gctagctgga attaccgctg tggctttaaa acaattgtaa ccgccaatta ctctagtttc
    25861 accattcatt tgccaattat aagttaccaa cgaatctttt actaaaaact ctttccccat
    25921 aaattctttg tcaacggtgt acattttttc ctttacattt ttataatacg tgccgccgcc
    25981 gcccataaag gaagacatca tgcgcattcc gccagcttga ttgggtgcgt ctagcttttc
    26041 ttcttcctta tatatagatg ccgatttatc aaaattgagg ataaatgttt tttcaaacat
    26101 cttcttcatg cgttcttcta tcattttttg catatcgggc gttatgtttt tattaccctc
    26161 cattctcgat ttaaaatcag aagtactggt ttttgattca taaactgcca tgccttgaaa
    26221 ttctttttgg gcaacagcta cgaacgtaaa gaggaagaaa gtaatgattg ctagattttt
    26281 catgtgtaaa gatttattta gagaaacaaa tatgacgaaa gattcgctta tctgttacca
    26341 attaagtgtt aaaaataatt tagactctgt ttttttttaa atggtttagt gtaaattagc
    26401 attatgatta aaaaagttat cttcatccta tttttattag tttgcccgtt tagtatgagt
    26461 caaaaactga caacaacact tgtcaattcc actccactag cagccgattc ctttttgggt
    26521 attgatacta tgggcaatta ctattttata aaagacaacg tgttcacaaa aaaaaatgct
    26581 tcacaaacat ggcaatataa aaatgtggct cttggcaaaa tcacttcagt agatattctc
    26641 aatcctttga aaataattat tttctacgaa aattttaaca ccactattct cttagacgca
    26701 caattgaatg aaatcttgca aaaaaacttt tctatcgctc taaaagaaac actcgtttct
    26761 catgttggct tggcttctaa taatcgattg tggttgtata acaatctgac aaactcaatt
    26821 gaactttatg attatacctc caatgcaagc caaactgtgg gacaaccttt gacccaaaaa
    26881 atagtatatg cccagaccaa ttttaatacc ttttattgga tagacgaaat gaataacctc
    26941 tatacttgtg atgtttttgg caaagttact ttgatgtgca atgttccagc atatgaaaaa
    27001 attcaaatca ttgatggaga aagacttctt ttttcaacaa ataataagtt atatctatta
    27061 aataatacct taaaatcaat aattgaaata gaaattgttg agaactcctt tgagaatttt
    27121 tattgtaaag accaaaattt agctattttt accaaccaac aaattagcaa ttataaaata
    27181 atattaccat aatgcacata gcaattgcag gcaacatagg cgcaggaaaa acatctctca
    27241 cacgttcatt ggcaaaacac tttaaatggg aacctcattt tgaagacgta gtggacaatc
    27301 catatttgga tgatttctat catcaaatgg aacgttggag tttcaatctt caaatttact
    27361 tcttaaacag tcgttttaga caaatcctac agatacgcga aagcggcaaa aacattattc
    27421 aagatagaac aatttacgaa gatgcacata tttttgctcc caatttgcac gctatgggtt
    27481 tgatgaccaa ccgcgacttt caaaattatt cttcgttgtt cgaattgatg gaaaatttag
    27541 tgggtccacc tgatttgtta atctatctaa gaagttctat ccctaatttg gttggacaaa
    27601 ttcataaacg tggtcgcgat tatgaaaata ctatttcgat agattatcta tcccgtttga
    27661 atgagcgata tgaagcatgg gtacactctt acgacaaagg aaaattatta attattgatg
    27721 tagataacat tgattttgta aacaatcccg aagatttagg gatggttatc aataaaattg
    27781 atgctgaaat aaacggtttg ttttaatcct ccccaacccc tccaaaggag gggctttttt
    27841 tactcttttt atttcctaaa atttatgcca ttagttaacc ctctttcgcc agaacaagac
    27901 caagaaacca aagaacttgc agttttcttt aacgaaaccc taggcttttg tcctaattct
    27961 gttttgacga tgcaacatcg tcctgccatc tcaaaagcat tcatcaattt aaacaaagcc
    28021 gtgatggaaa accacggacg agtaacctca gcactaaaaa gaatgattgg ctgggttacc
    28081 agtaatgctg ctggttgccg atattgtcaa gcacacacca ttcgtgctgc cgaacgctat
    28141 ggagcaaacc aagaacagct ggataatatt tgggaatatg cctcgcatcc tgcgttttcg
    28201 gatgccgagc gggctgcttt aaactttgct ttggcagctt cggtcattcc aaatgctgtg
    28261 aacgaagcta taaaaaatga cttatacagc cactggaacg aaggcgaaat tgtagaaatg
    28321 ctaggtgtta tttcactttt tggctacctc aacagatgga atgactctat gggaaccact
    28381 attgaagatg gtgccgttga aagtgccgaa caatatctgg gaaaaacagg ttgggaaaag
    28441 ggaaagcatc aataaactaa aactataagt aggcattgat attcaacatc ccgtttttat
    28501 gatgtgattt tgaactgttt tgggtcgcaa agcactgttt taaagttaaa atccaatatc
    28561 ttttggattg aacaaaacaa gagttttttt gtagttgttt ttaatccaag aaaaaaaaca
    28621 caaagcgcaa actttgtgtt ttttgaataa tatcataatc gtaaaaactt atcgaatctc
    28681 ttgattcaaa attaaatcga tgtataagtt gattttgttt ttcatttctt ttcgatgaga
    28741 aataaaatct aagaaaccgt gttccaataa aaattcagca gtttggaaac cttctggcaa
    28801 atcttttcca gtagtatcac gcactactct tggcccagca aaaccaatta acgcaccagg
    28861 ttcggagata ttaatatcac ctagcatggc ataggaagct gtagttccac cagttgttgg
    28921 gtctgtacaa agtgatatat agggtatttg tgcatcggca agctgtgcta attttgcaga
    28981 agtttttgct aattgcatta acgaatatgc tgcttccatc attcgcgcac cacccgattt
    29041 tgaaatcatt acaaaaggca atttatgttt gatagaataa tcaattccgc gagcaatttt
    29101 ttctccaact acagcaccca ttgaaccacc aataaaagca aaatccatac aacaaaccac
    29161 caaatcttta ccttttgatt ttccaacacc tgtacgaaca gcgtctttta gttttgtttt
    29221 ttcctgaacg tcttttaaac gttcagaata ttttttagta tcaacaaagt gtaaaggatc
    29281 tttggatgtc attttagcat ctaattcttt gaattcattg ttgtcaaaca aaatttcaaa
    29341 gtattcttta ctaccaatgc gaacatgaaa atcatcttct ggacttaccc aaagattttt
    29401 ggctaattcg tctgtatcaa taatttttcc tgttggtgat ttgtaccaaa gtcctttagg
    29461 aacatctttt ttatcttcgg tagcggtagt gatacctttt tcttttcttt taaaccaagc
    29521 catataagtt tattgtttat gatttgttgt ttgttgtggg ttttggtgta aactacaaac
    29581 tttgttttat aaagtgttta cattgtttaa atcagcaaat gcttgttcta atcttttgtt
    29641 gaaagtcatt tcaccttcgc gaatccattt tcttgggtcg taatgttttt tgtttggcga
    29701 atcggaacct tcgggacttc ctatttgtgt tcttaaatag tcaatttttg aagtcatata
    29761 atcgcgaatt ccttctgtaa aagcaaattg caaatcagta tcgatattca tcttgattac
    29821 gccatatcca atcgcctctc taatttcttc tatagtagag ccagaaccac cgtggaatac
    29881 aaaatcaaca gggttatttc ctgtattgta tttgttttga acataatctt gagagttttt
    29941 caagattttt ggagtcagtt ttacgtttcc tggtttgtaa accccgtgaa cgtttccaaa
    30001 agcagcagca atagtgaagc gtggagaaac tttcattaat tcttcataag cataggcaac
    30061 ttcttcgggt tgtgtatata attttgagct atcaacatca gaattgtcaa caccatcttc
    30121 ttctccacca gtaattccta attcaatttc taaagtcatg cccattttac tcattcgagc
    30181 taaatattct ttgcaaatct caatattttc ttcaataggc tcttcggata aatcaatcat
    30241 gtgagaagaa tataaaggtt tgcccgtttc tgcaaaatgt ttttcagaag cgtctaacaa
    30301 accatcaatc caaggcaaaa gtttttttgc acaatgatca gtatgtaata taactgttgc
    30361 tccataagcc tcagccaaag aatgtatgtg ttttgctcca gcaactcctc ctgcaatggc
    30421 tgctttttca ttttcattag acaatccttt tccagcatta aagtgtgccc cgccatttga
    30481 gaattgaata attacaggag cattaagttt tgcagccgtt tctaaaacac tatttatact
    30541 gctagaaccg attacattta ccgctggtaa tgcaaatcct ttttcttttg cgtatgcaaa
    30601 aattgcttga acttcatcgc ctgttgcaac tccgggttta atattatgag ccatttgttt
    30661 tggaattttt agttaataag aatacaaaaa taggaatttc taaatttaga acggataatt
    30721 tatcccaata tttaatactg atttatcgaa tcgaatgtct ttaaaccatt tttcattttc
    30781 gggatttgca ggattatacg ttttaaatcc aaagtcagtt cttaatacaa agaagttaaa
    30841 atcgtatcgc aatccgaaac cagttccaac cgcaatatct tttaaggagt ttacaccact
    30901 aaaaacggat ttttcatttg aaatattgtc taaaacgttc catatattac ctgcatctac
    30961 aaaaacagca ctgtataagt ttccaaatac attaaatctg aattcgctac taagtgttag
    31021 tttcatattg gcttcgttaa aatccaaaat actttcactt gaaccaggtc caagactgta
    31081 agattgccaa gctcgaatat cattagatcc tccaccaaaa taacttcttg aaaatggaat
    31141 gctatttgag tttccataag gaatggctat accaaaaaat cctttgacag caaaaacttg
    31201 tttacttgat aaaccccaat ggcgaatgta ttctagttct gattttatat attgagaata
    31261 ttcaacttca aaaaatgtgt tggcacctga ttggcttttt aattgtttgg aaattcttga
    31321 aaataaagac aataaatttc ctgcactttc aaattttgtt ttaaaggcat aaaaattatt
    31381 atcaaatata tccttttttg atgttttaga gtaagtaata tttgaagaaa aaatcaaatt
    31441 attttcagta agtcttactc ttctttctaa tatacttaaa acagatttta agtcaacggt
    31501 agagagatta agacttgggt cggtaaaagc agcaactaaa aaaccacttg ttccgtcatc
    31561 aatttttaaa tttccattag catctaagaa atctgtactt acaggataat tttgtgcaat
    31621 atcatttagt gcgttatagg aagaagtata aatattaaag taatttgttg gatttaagtt
    31681 ttttacaaat tgaatattta tcaagtcaaa tttaaaactt acgtttcttt ttggcgtcca
    31741 attgtatgta aatgagcttg taaaattttg tttatctaaa ccaacatttc tttgcttcgc
    31801 atatccaaca ctcaaagtag tatatggaat cattcttttg ggtattatct tttccgtttt
    31861 aaatggtaaa atcaattttg ggaagtttaa tttggcatca atcccaattt cagatatatt
    31921 gaagaaatta tcattaggat tcgctaaatc tcttgaggaa ccaatatttc ctctaaagcc
    31981 taaatcaaat gtttcggtac cattaaaaac atttcgaata cttaaaaatg tagttcccga
    32041 aataccaaaa tcttgaatat ttgaatgtgt aaaatcggtt gaaaaaccaa aagtatattt
    32101 ttttctagga gttaaataaa tgttggcaat tagtccgttt tgatcatttt tatcttcaac
    32161 atattgtatt aatggataat taaaaacttt caaatttgtt aaatatcgag aagttatagc
    32221 cgttttaaag tctgaaaaat agttgtcttt agcaataaaa acaccatttg ttattgattt
    32281 taatctgtat ttcaatttgt taacactgta tatattgaaa tttttataaa cagcactatc
    32341 tgttattttt gcatttggat ttgccgtact atgatcggta aaaatattga ctctattaat
    32401 tttatataat ttaaaagggg aaatcacggt agaatcattt actcttgaaa cttcatcgtc
    32461 aattaaaata gtaatttttg gtttgtgtcc tctttttata gtatcaattt cataattgat
    32521 atagttttgt tgaaatttga aagcaccatt atttctaaaa tcttctgtaa ttctattgcg
    32581 ttcgttttca aaattgtttc tattgaattg atctccaact ttaatatagc tattgttttt
    32641 cttttcttga tagatagaat ccaaaacttt ggtagtaatt attgttgcta ttgaatcaat
    32701 aatatatggc tttcctttag ttacactata gttcaatttt gcttttttgg cagcaatcgt
    32761 gtcaatagta aactttgctt ttgcatcaaa ataaccaata tttgaataat acaatcgtaa
    32821 tcttcgcaat gattttttag tactagccgt atcaagaatg actggtgctt cgcctgtttt
    32881 catcaaaaaa ttatgaatac cagagtacca aaaggattct cccaagcgtt ttacttgttt
    32941 tctggaaagc cattttgatt ttcgataata cttttttgga ttgttaataa attttgctct
    33001 ataaatagaa tccgtttttt gcttggctaa attgtatata tttaagcgta atttgtatcc
    33061 aagaatagag ctgttttgtt tttgatacaa ttgattaaaa acttcttccg atttttcttt
    33121 ttgctcatca acctcaatcg tattatttac aaggagtttt tttccatcgg gaactctttt
    33181 tgttgtatta caactgaaaa ggaataatcc tgatagaata ataaatacta tttttgtgca
    33241 gttaaatttc aaaggaagca attataaatt caaaaataca ttttttatgg ttagtaaaaa
    33301 ccaaataaaa ttgataaata gtcttcagca aaaaaaatat cgaattgaac accagctttt
    33361 cattgcagaa ggtgtaaaag taattcaaga attgctacaa tcaaatagtg tattagaaca
    33421 tttatttgct acaaatgact ctttttctac ttttaaagtt caagcaaaaa cagaaattac
    33481 tactcaagaa atgaagaaaa tttcgggact ttcttcgcca agttcttgtt tggctttatt
    33541 tcgaattcca aaaccaacga atatcaatcc tatgggctta attgtggctt tagacgccat
    33601 tcgtgatcct ggtaatttag gcaccattgt gcgtctttgt gattggtttg gtgttactca
    33661 aatcgtttgt tcaaattcaa ctgttgattt gtataatccc aaagtagtac aagctactat
    33721 gggttcaata gcaagggtaa atgtaaatta tttggattta aagaccttta ttatcgaaag
    33781 taatttgcct gtttttggta cttttatgga tggagcaact atttataacg aaaagttacc
    33841 caaagaagga atcattgttt ttggtaacga agccaatgga atctccttag aaattgaaaa
    33901 aaatgttaaa aataaaatag ctattcccca atttggaagt attcaaaaaa cagaaagttt
    33961 aaatgtggca acagcaatgg caattgtgtt gagtgaattc agaagaaatg attaatgaaa
    34021 tgtaaaattg attaatattg ctctggtttt catcgattga atatttcctg tccaaggaga
    34081 atttgggtca ttatctcgaa ttaattcatc attaagacca aaaacacccc gaattgaagg
    34141 cgaaaattta aaatattcaa aatataaatc aaccccaaaa ccaagttcgt agttttgtgt
    34201 ccacgatttt actctaaatc tttgctgaag gttatcatca tcagattttg aattacttga
    34261 caaattcaaa gtagcagaaa ctccacctac taagtatgga cgaatgtttc ctgttcttag
    34321 ggaagagaat tttagcaaca aaggaaagtt gatgtaagtt gcttttactt ctcgaagacc
    34381 atctctatcg ttggtaaatt gagaatatgt tattgttctt gaggcataat atagtcctgg
    34441 ctcaaatcgt aagctcacat attcgtgcaa acgcaaatcg ccaactaaac ccacattaaa
    34501 gcctgtttgc ccttttatgt caatattatt ttgttctact ttgtaatctg ttttaaaatc
    34561 aaaactgcta aatccaagaa aatatcccca gtaaactctc tgtttgtcaa agttttctaa
    34621 atttacaatt ggatctttgg aaaacatacc ttttccaaat tgcgcatgca attgagatgt
    34681 tgtagagata agaattaaaa aaaataattt tttcattagt aggctttgca aagtttgaat
    34741 tatttttttg ttgctgtata aattgttgca acaccaaatg tttgtggtaa cgctttacat
    34801 tctataaacg aaactttgcg taaaatattg tttaattttt caccaaaagg aaaatttgcg
    34861 gcagaagttg acaaataact gtaagcggat ttgtctttag aaaagatttt cccaataatt
    34921 ggcaatatca tttttgagta aaaatagtag ccttgtttga aaggaaattt ggttggcacc
    34981 gaagtttcaa gaattacaaa aattccattg ggcttcaaaa cacgataaat ttcggctaaa
    35041 cctttttcta atgtttcaaa atttcggatt ccaaaggaaa ccgtaatcgc atcgaaagta
    35101 ttgtcttcaa aagggatatt ctcgctatca gcttgaatca tctctatttt attggaaaga
    35161 tttttttgat tgattttttg tttgccaatt tcgagcattc ctgctgagag gtctaaacca
    35221 acgatttttt cagctgtaga atctgccatc ataatgacca aatctcctgt tccggtagca
    35281 atgtctaaaa tagtttttgg atttttatct gctactaatt gtactacttt ttttctccat
    35341 tttacatcga ttcctaaaga aataacacga tttaattcat cgtaattccc cgaaatatta
    35401 tcaaacattt gagtaacttg ttcttttttt cctaaatcag aatctttata aggtgttatc
    35461 tgctttgaca ttgtgtaatt ttatgcaaat ataaagtata ttatttacaa atttttatat
    35521 tctattcggt ctttctgcta taggttaaaa aggtaaaatc aaagttatgc ttgttgtctt
    35581 ttggatgaaa ttcttcaaat tctaattgcc ataaatccat attcagtttt gggaaatacg
    35641 tatctgcttc aaaagtatga tgcacacgag tcagttcaag tttgtctgct aaatttatag
    35701 attgattata gatttcacca ccgccaatta tataaatagc ttcatcgatt ggacatgcag
    35761 caattccatc tttaatagag ttaacaatga tgcaatcttt tggcgcataa tttttttgtc
    35821 gggtgacaat cacatgtgtt ctatttggta gtggttttgg aaaactttca aatgtttttc
    35881 ttcccattat aataaagtga cccgaagtta tcgctttaaa tcttttaaaa tcatctggca
    35941 aatgccaaat caattggtta tcttttccta actcgttgtt ttcgccaatg gctgcaataa
    36001 gtgtaatcat tcgtttgttg ttgctttttt ttctaaatct gcatttagtt tttcaatttt
    36061 cttggtttgc aattgaatca atttatcaat ttgagtgcgt tcccaatttt tattcataaa
    36121 agcatcggta atgaaaactt taattatatg aagcacaaaa ataaaacccc aaattgtaac
    36181 tgcccaaatc caccactttg tttcgggata aaaccctaat agattgttgg caacaattaa
    36241 aaaaatactt cctaaaataa ataatacgaa atgaaaatac aaacgttttt tttgtttgat
    36301 gcgatttctt gcatattcat aaagttcgtg ttgatctttt tccataagac gatttttgag
    36361 gtttaaagat agtttttttt tcgaaaattt aaaatagtct aattaataaa tcgcagtaag
    36421 aaaacgtttt cttaattaag gatgtagaat tttaatgcgt aaaaattaac atatcacttt
    36481 aaaaatggct tagtttttag taattcccta aaaatgaaac ggaatgtaaa aggtattaaa
    36541 aaaagctttt actttgcatc agtaatctat aaatgaatta gttatggcta tcaaaaaaca
    36601 atttataaaa tcaaaacctg tttgcaaagt taccttttct atcgaagcta aagaggcaaa
    36661 tactgctgct gtagttggag actttaataa ctggaacccg tcagaaggtg aattgtcgaa
    36721 attaaaaaac ggtactttca aaggtctttt tgaagttcct aaagatgctt cttatgaata
    36781 taagtatgtc gttgatggaa cttatgtaaa tgatcctgaa gcagatggtt atgtatataa
    36841 cgattttgcg ggtgctgaaa acagtgtggt tactgtatag ttttattggt ttttgtaaaa
    36901 aatccgtctc gttttagtac ttgacggatt tttttatatc taaaaattaa tcattatata
    36961 aaaaatagta cgcttctaca ttatcaagat atactccatc aaaacctgta tcaactattt
    37021 ttttagcata tgaattatcg ttgccataaa taatgtcttg ccattcacta ttccaatatt
    37081 ctacccaaat ttcttggtca taatctttat agggtttttt gagccatttc ggattgttaa
    37141 tcttccaatc tcttctccaa taagttctat atttttcggc agcaccaata ttcatataac
    37201 caactaccaa tcttttaccc ccattttttt taatttttaa tttctctatt tcctctttgt
    37261 taaattggac gccattgtgg tataaatcga taaaaatcaa atcataattg gtgtttttta
    37321 aggctgttat aaatgctgat ttgctataat aattactgtt attgattata taaagaaaat
    37381 tttttatgtc acttaatttt tcaacattat ttttattttc attatgaact ttcgtcggaa
    37441 ttaaagtata atgataattc tcttccgttc ttgtaaagca taaaaagcca gatttctcat
    37501 tgcgctcata agaatctttt accaaagctg tatctttcac aaattctgaa actataattg
    37561 tttcagattc tttgatttta gacaataaat ctatcctgta tgtatcgggt tttaattcac
    37621 cattataaaa taattcttca attccaaaac catcaatagc ctctaaaaat ttttggtcta
    37681 attttttatc cgtttttcca ttttcaaaag ctaattctgc accattttgt ggaatgatga
    37741 taaaatttgg gttaattttt cgagtataat ctgaaatgtt aataatgaaa tcttgcattt
    37801 tttcagcagg attttttttg ggttttgtat tcaaataaca tccaacagaa acacttattg
    37861 aagaaaaaag gagtaataat attgtttttt tcaaaatcat ctttttatat tgctactaca
    37921 cctttaatat gcggatgtgg attataatca accaatgtaa aatcttcaaa agtaaagtcg
    37981 aaaatatttt ttacttccgg atttaatatc atttttggta acggtagaat aggtcttgaa
    38041 agttgcaatt ctacttgctc aaaatgattg ttataaatat gtgcatctcc aaaagtgtgg
    38101 atgaaatcgc cgacttgcaa attacaaact tgagcaatca tcatagttaa taaggcatat
    38161 gaggctatat taaacggaac accaaggaaa atgtcagcac tacgttggta taattgacat
    38221 gatagttttc cgttggcaac gtaaaactga aaaaaggcat gacaaggtgg caaagctgct
    38281 tttccgttgg ctacattttc tgaaaatgtt tttgacgtgt caggcattac cgaaggattc
    38341 caggcggaaa ctaacattct tctgctgttt ggattgttct ttagggtttc tattaattcg
    38401 gttatttgat caatctcttc actattccaa ttgcgccact gatgaccata aactggtccc
    38461 aaatcgccat tttcatcagc ccattcatcc caaattttta ctccattttc ttttaggtaa
    38521 ctgatatttg tatcgccttt taaaaaccaa agtaactcat aaactattga ttttaagtgc
    38581 agctttttgg tagttaccat tggaaagcct tcatttaaat caaaacgcat ttgataacca
    38641 aaaacacttt ttgttcctgt tcccgttcta tctccttttt gacaaccgtt ttctaaaaca
    38701 tgttgcatta aatcgtggta ctgtttcata aataagttta agagtttaaa ggtttaaagg
    38761 ttcatattcg atagtattat tgagctaggt ttgtcaaact gaacattgtt attgctcact
    38821 actatcaaat actaactttc tcttttcgaa atttcatctc taattctagc tgctttttca
    38881 taatcttcgt cttgaactgc tgcttctaat gatgaatgca attctgacaa actcaattta
    38941 gaataacttt cactagaatc aatatctgta ctatcaagac caaaggtttc aggagttgat
    39001 aatacgccat catcttctac ttctgtttca tttgggttgt ttaaataaat accagctttg
    39061 tctaaaatgt ttttatatgt aaaaattggt gcttgaaaac gcagtgctaa tgctattgca
    39121 tctgaagttc ttgcgtcaat aatttcttct attttatctc tttcacaaat gatgcttgaa
    39181 tagaaaacac catcaacaag tttgtgaata atcacttgtt ttactacaat atcaaatcgg
    39241 tcggcaaaac ttttaaataa atcatgtgtt aatggtcttg gcggttttat ttctttttct
    39301 aaagcaatag caatagcttg tgcttcaaat gcaccgataa ctattggaag ttttcgttct
    39361 ccatcaactt cattcaaaat caatgcataa gccccatttt gggtttgact atatgatatt
    39421 ccttttatgg atagttttac taaactcatt tagattgttg a
//
LOCUS       DAOOBH010000010        38715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_1357_length_38715_cov_6.565856, whole genome shotgun sequence.
ACCESSION   DAOOBH010000010 DAOOBH010000000
VERSION     DAOOBH010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 38715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38715
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1357_length_38715_cov_6.565856"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK218_02490"
     CDS             <1..514
                     /locus_tag="PK218_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW97408.1"
                     /translation="PFVVLFAVPLSFIGALLALALTNNSLNIFTILGIIMLIGLVCKN
                     AIMLVDYTNQRRAAGESIRTALIQANHARLRPILMTTIAMVFGMLPIALASGAGAEWK
                     NGLAWVIIGGLISSLFLTLIVVPVIYEIMEKIVGKFSKGEKIDYEKEMVADYVHQELS
                     DDGFNPKHTL"
     gene            complement(579..923)
                     /locus_tag="PK218_02495"
     CDS             complement(579..923)
                     /locus_tag="PK218_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-binding protein"
                     /protein_id="HPW97409.1"
                     /translation="MRKSMIIAAFLITGYTFAQEVKPVLEQNGDLVKATYYYENGQIQ
                     QEGFFKDGKLNGQWVSYDESGNKKAIGEYANGEKTGKWFFWNDKSLSEVDFSKSKISN
                     VKAWTQEAIVNK"
     gene            complement(1099..2091)
                     /locus_tag="PK218_02500"
     CDS             complement(1099..2091)
                     /locus_tag="PK218_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPW97410.1"
                     /translation="MKKIVSSLFLVLMVTFSFAQETMTFEANVINKNGDVLFIKENRV
                     IVKEIKVDDKGAFKATFPIKEGLYQLFDGVEYAQLYLKNGYNLKMTLDGKMFDESIKF
                     DGEGSKENNFLAKQAMEEQKYPYDQVLTMDEENFKKAVEEKRTTDNKKFEAAGLDVKL
                     LEVLKKSAEMNLAGLTKYYNQISANKKLNNIQSPNFVYENHAGGKTSLESLKGKYVYI
                     DVWATWCGPCRAEIPSLKTLEESMQGKNIEFVSISVDVEKDYEKWKDFVKDKSLGGTQ
                     LIADNNWSSDFIKAFGINSIPRFILIDPTGKVVDADAKRPSNPKLKEQLEGLLK"
     gene            2215..3960
                     /gene="aspS"
                     /locus_tag="PK218_02505"
     CDS             2215..3960
                     /gene="aspS"
                     /locus_tag="PK218_02505"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPW97411.1"
                     /translation="MFRSHNNGELNATHITKEVTLAGWVQKSRDKGFMIWVDLRDRYG
                     ITQLIFDEQRTDKTVFEKAKSLGREFVIQVKGTVIERESKNLNMPTGDIEILVSELIV
                     LNEALLPPFTIEDETDGGEDIRMKFRYLDIRRNPVKNSLLFRHKVAMEVRKYLSDLDF
                     CEVETPYLIKSTPEGARDFVVPSRMNEGQFYALPQSPQTFKQLLMVGGMDKYFQIVKC
                     FRDEDLRADRQPEFTQIDCEMAFVEQEDILTVFEGLTRHLLKKLKNVEVAEFPRMTYD
                     YAMKTYGNDKPDIRFDMKFGELNQVAQHKDFGVFNSAELVVGIAAPGCASYTRKEIDA
                     LIDWVKRPQVGASGMVYVKCEEDGTFKSSVDKFYNQEDLKKWAEITNAKSGDLILVLS
                     GNADKTRTQLSALRMEVATRLGLRNPTEFAPLWVIDFPLLEWDEESGRYHAMHHPFTS
                     PKPEDMSLIATEPGKVRANAYDMVLNGNEIGGGSIRIHDKDLQSRMFDLLGFSKEEAQ
                     NQFGFLMNAFEFGAPPHGGLAFGLDRLVAILGGQETIRDFIAFPKNNSGRDVMIDAPS
                     AIDNKQLEELHIKLK"
     gene            4091..4291
                     /locus_tag="PK218_02510"
     CDS             4091..4291
                     /locus_tag="PK218_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPW97412.1"
                     /translation="MRTGKVKFFNESKGYGFITDDETGNDIFVHASGVQNNTLNEGDK
                     VSYEEEEGRKGKIAAQVTPIED"
     gene            4480..4845
                     /locus_tag="PK218_02515"
     CDS             4480..4845
                     /locus_tag="PK218_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPW97413.1"
                     /translation="MQSNQLDYLPIVMQAGLAIGFVVLTIIGSSFLGPKRHSKNKDKN
                     FECGIESVGNARIPFSVKYFVVAILFVLFDVEVIFLYPWAINFKELGMEGMIKMVIFM
                     ALLLIGFFYIIKKKALEWE"
     gene            4859..5404
                     /locus_tag="PK218_02520"
     CDS             4859..5404
                     /locus_tag="PK218_02520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HPW97414.1"
                     /translation="MSTSNPNMVAPPEGVVGEGYFATKLNDVVGLARANSLWPLPFAT
                     SCCGIEFMATMASHYDLARFGSERVSFSPRQADMLMVMGTISKKMAPILRQVYEQMSE
                     PRWVIAVGACASSGGVFDTYSVLQGIDKVIPVDVYVPGCPPRPEQIVDGVMQLQELVK
                     NESVRRRNSPEYKELLASYNL"
     gene            5406..5930
                     /locus_tag="PK218_02525"
     CDS             5406..5930
                     /locus_tag="PK218_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPW97415.1"
                     /translation="MALETTYIQEKLSQKFGSNVLNFEMKRDIFSLEATSETVHEVIQ
                     TLKEDESLNFHFLTDVCGIHFPDNDENHQFAVVYHLHNWIENMRIRVTTFLKGENPEV
                     SSIIDLFTSANWMERETWDFYGINFIGHPQLKRILNMDEMVSFPMRKEFPLEDSGRTD
                     KDDRFFGRTIKQIK"
     gene            5949..7184
                     /gene="nuoD"
                     /locus_tag="PK218_02530"
     CDS             5949..7184
                     /gene="nuoD"
                     /locus_tag="PK218_02530"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HPW97416.1"
                     /translation="MSELLLPPEHRYAKIIEETRNSDGSELTILNLGPTHPATHGIFQ
                     NILLMDGERIIDAEPTIGYIHRAFEKIAENRPFYQINVLTDRMNYCSSPINNMAWWMT
                     VEKLLKIEVPKRAQYLRVIVMELARITDHLICNSILGVDTGAYTGFLYVFQFREKVYE
                     IYEEICGARLTTNMGRIGGFERDWSEEAFRKLDVFLTDFPVAWKEFENLFERNRIFID
                     RTVNVGPLTAESAMDYGFTGPNLRAAGIDYDVRKAQPYSSYEDFEFDVPVGKSGDTYD
                     RFCVRNAEVWESLKIIRQALDKLPEGPIHADVPDYYLPPKEEVYNNMEALIYHFKIVM
                     GEVPVPVDEIYHAVEGGNGELGFYLITDGSRTPYRLHFRRPCFIYYQAYPEMIKGSML
                     SDAIVILSSLNVIAGELDA"
     gene            7260..7790
                     /locus_tag="PK218_02535"
     CDS             7260..7790
                     /locus_tag="PK218_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPW97417.1"
                     /translation="MEISHIKQEINITESLMTRINELISHYPADKRKSALLPVLHEVQ
                     DAHDNWLSIDLQNKVAEILEIQNIEVYEVVTFYTMFNQRPIGKYMFEFCQTSPCCQNG
                     VEDLMDYTCEKLGVKVGEPTKDGNFEVRGVECLGACGYAPMMQLGDFYQEHLTKEKID
                     QLISDCKEGKITLHDK"
     gene            7795..9162
                     /gene="nuoF"
                     /locus_tag="PK218_02540"
     CDS             7795..9162
                     /gene="nuoF"
                     /locus_tag="PK218_02540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPW97418.1"
                     /translation="MGRKILLDKINVPGIKTYEVYRKEGGYASVEKALKKMTPDEVVE
                     EVKTSGLRGRGGAGFPAGMKWSFIDKKSGKPRHLVCNADESEPGTFKDRYLMEYIPHL
                     LIEGMITSSYALGANLSYIYIRGEYMWVYKILERAIAEAKAAGWLGKNILGSGYDLEL
                     YVQIGGGAYICGEETALIESLEGKRGNPRIKPPFPAVEGLWQNPTVVNNVETIAAVPW
                     IVNNSGADYAAIGIGRSTGTKLISASGNIKNQGVFEIDLGLSVYEFMNSDEYCGGMQT
                     DRPLKALVPGGSSVPILPANLIYKTAAGEDRLMSYESLSDGGFATGSMLGSGGFIVYD
                     ERACIVRNTWNFARFYHHESCGQCTPCREGTGWLEKVLWRIENGQGREEDIDLLWSIQ
                     AKIEGNTICPLGDAASWPVAAAIRHFRDEFEYHVRFPEKIKNRDHFVNEPFEKVKHLV
                     AKQTV"
     gene            9247..10296
                     /locus_tag="PK218_02545"
     CDS             9247..10296
                     /locus_tag="PK218_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPW97419.1"
                     /translation="MKVTIDGQEIEVAPGTTILQAARMIGGESVPPAMCYYSKLKGTG
                     GKCRCCLVDVTKGSDNDPRPMPKLMASCVTGCQDGMEIASKSSERVKEARNAVTEFLL
                     INHPLDCPVCDQAGECDLQNLSFEHGKLQHRFIEEKRTFEPEDIGDKIQLHMNRCIVC
                     QRCVEVADQLTDGRVHGVLNRGDHSQISTCVSAAIDNEFSGNMIDVCPVGALTDKTFR
                     FKSRVWFNKPFNAHRDCDKCCGKTTLWMFGNEIQRVTARKDEFHEVEEFICNTCRFDK
                     KEVSDWTIEGPRKFEKDSVINQNNYTRKMDKVVINTEEGILAGRAIDRKKISMAEIDY
                     NEKIDGTNINSNKNG"
     gene            10289..11320
                     /gene="nuoH"
                     /locus_tag="PK218_02550"
     CDS             10289..11320
                     /gene="nuoH"
                     /locus_tag="PK218_02550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HPW97420.1"
                     /translation="MDSAFIIEKSIFIVVIFAVTMVMAMYSTWAERKVAAYLQDRVGP
                     NRAGWGGLLQPLADGMKLFAKEEFEPNTPNKFLFYVGPAIAMSTALMTSAVIPWGDKL
                     EIFGRTVILQATDIDNALLYVFAIISIGIYGIMIGGWASNNKFSLMSAVRAGSQMVSY
                     EIAMGLSIIALLMMTGTLSLREMSMQQAGMNWNVFYQPVGFLIFLICAFAETNRTPFD
                     LAECESELIGGYHTEYSSMKMGFYLFAEYANMFISSTILAVLYFGGYNYPGMQWMTEH
                     AGVNIANILGIIVLFIKICGFIFFYMWVRWTIPRFRYDQLMHLGWKILIPLSIINIVI
                     TGICLLAFN"
     gene            11333..11878
                     /locus_tag="PK218_02555"
     CDS             11333..11878
                     /locus_tag="PK218_02555"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HPW97421.1"
                     /translation="MSTSIQNISLSGHKKQVSNKEMTFWERLYLIAIIKGLLITIKHF
                     FKKKATIHYPEQVREFSPVYRGKHTLKRDELGRENCTACGLCALSCPAEAITMKAAER
                     KKGEEMLYREEKYAEIYEINMLRCIFCGLCEEACPKDAIYLTLSKELVPSLYNREDFI
                     YGKDKLVMPLDVALANSKKLH"
     gene            11844..12392
                     /locus_tag="PK218_02560"
     CDS             11844..12392
                     /locus_tag="PK218_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPW97422.1"
                     /translation="MLHWLILKSYINMSTVLIIFYILSAITLSTAFLTIYTRRPIHSA
                     IYLVLCFFSIAGHYLLFNAQFLAIVHVIVYSGAIMILFLFTIMLMNLNNEDEVHKPRI
                     TRLGAIIIFVLVSIIMISVFINSKTIMGDYDNSGVDYQSIKVLGKVLLNEYMVPFEFA
                     SILLLVAMMGTVLLSKKEKSGK"
     gene            12392..12712
                     /gene="nuoK"
                     /locus_tag="PK218_02565"
     CDS             12392..12712
                     /gene="nuoK"
                     /locus_tag="PK218_02565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPW97423.1"
                     /translation="MGNILNQIGIENYIFLSVLLFCIGVFGVLYRRNAIIVFMSIEIM
                     LNAVNLLFVAFSTYHQDAEGQIFVFFSMAVAAAEVAVGLAILVSIFRNLGSIEIDKLK
                     NLKG"
     gene            12717..14594
                     /gene="nuoL"
                     /locus_tag="PK218_02570"
     CDS             12717..14594
                     /gene="nuoL"
                     /locus_tag="PK218_02570"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPW97424.1"
                     /translation="MEKNLALVLLLSPFIGFLFNVFFGKKVSKGLSGAIGTIAVVVSF
                     LVSICFFTQLNQNGKGFEISLFDWIQVSNFKLDFGILLDQLSLLWLLFVTGIGSLIHL
                     YSISYMHDDENMHKFFAYLNLFIFFMITLVVGNNLLVMFIGWEGVGLCSYLLIGFWYK
                     NQPYNDAAKKAFIMNRIGDLGFLIGMFLIAYVFSTLNYTEIQTLVIKGETNSSLLSVA
                     ALCLFIGACGKSAQLPLYTWLPDAMAGPTPVSALIHAATMVTAGIFMITRMNFLFDIT
                     PDVQNIIAIVGAITSLVAASIGLLQNDIKKVLAYSTVSQLGLMFLALGFGAYNVAVFH
                     VITHAFFKACLFLGSGSVIHALHGEQDMRNMGGLKKYMPITFWTMMISTLAISGIFPF
                     AGFWSKDEILMTAFEHNTTLWVVASVASLMTAFYMFRLMYLTFFKEFRGTEEQKHHLH
                     ESPSLITIPLIILAVLAAIGGAINLPGSMWLNHFLEPILVAKHEAHELGAHEFMLMGI
                     ALAGAIIAIIWAYSKYIKQSFVPEEDTQMVGFYKTIYNKYYVDEFYTFLIVKPLNGLS
                     NFFRTTLEPALGQIVFSLGTLANGISNQGRKIQTGSVGLYLFAFVLGLCAIISYLFIA
                     R"
     gene            14605..16044
                     /locus_tag="PK218_02575"
     CDS             14605..16044
                     /locus_tag="PK218_02575"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPW97425.1"
                     /translation="MNVTLLLLLLLTGSIVTFLSGDKLASKIALLFSVSAFAVSLYLL
                     MLFNQGEIISYMSSWMQKPNVSFALKADGLSLAMVLLTTGLTPLIILSSFGNSYTNSK
                     NFYALVLFMAFSMTGTFLAADGLLYYVFWELALIPIYFIALIWGNGDAEKRKKAVVKF
                     FIYTLAGSLFMLVAFAYLYSKAGSFILEDLYQLRLSETEQFWIFFAFFAAYAIKIPII
                     PFHTWQAKVYQKAPTVGTMLLSGIMLKMGLYSVIRWQLPIAPDAAKTYMPYLIGVSVA
                     GVIYGSVVALRQKDLKKLLAYSSLAHVGLIAAGCYTLTADGLNGAVYQMIAHGFVIVG
                     LFFAAEVIFRRFETRKINELGGIRSQASKFTSMFMILVLASVALPGTYNFVGEFTVLY
                     SLSQINIWFAVFGGTTIILGAYYMLKMFQNVMLGDTNTKVFADVTLNETIVFVTIIGF
                     LLFFGLYPKPINELVNPAIQNILTVINRS"
     gene            16046..17422
                     /locus_tag="PK218_02580"
     CDS             16046..17422
                     /locus_tag="PK218_02580"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPW97426.1"
                     /translation="MQVLIAIASLGIFCLVAEIFNLRKILVPVSVIGLLAILGITLNF
                     WSISDSLDARYITMIASTGFSKAFISLFIILTIFILAMSNDYYKEHPTKFSDFIAIKI
                     FLLTGAVAMVSFTNLAMFFLGIEVLSISLYILAASNRLNIKSNEAGMKYFLMGSFASG
                     IILFGICLIYGATGYFDMVEIHDLSQGIGLPVWFPIGIALMIIGMLFKIAAVPFHFWA
                     PDVYEGSPTLITATMSTLVKVVAMATLFKLLNGLLTSIPPKVEILILIISILSMTVGN
                     IMALRQKNVKRMLAFSGISHAGFMLMTLLTMATAANNLLYYTFAYGLAGIAAFAVIIH
                     VTKNKEDEAIFNFNGLGKTNPLMAAALTASLLSMAGIPIFSGFFAKFMLFTDTIKSGF
                     IAIVIVAVINSIISIGYYFKLILAMYTKEATAEKESISSVYTIVAVLAIILNIIIGIN
                     PSLVLNLM"
     gene            complement(17497..18849)
                     /gene="mgtE"
                     /locus_tag="PK218_02585"
     CDS             complement(17497..18849)
                     /gene="mgtE"
                     /locus_tag="PK218_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPW97427.1"
                     /translation="MQFKISKELIEQIEQLILVKNDQELELLLNDMHHADIAEIFDEI
                     SIDDATYVFRILDSEKTAEILLELEEDVREKILRGLSAKEIAEELDELDTDDAADIIN
                     ELPESKKEQVISELEDLEHAKDIVDLLRYDEDSAGGLMGKELVKVNENWNVLTCVKEM
                     RIQAENVTRVHSIYVVDDENRLKGRLSLKDLLTTSTKTPISEVYIPKVDSVKVNMPSV
                     DVARIMQKYDLEAIPVVDEMGRLVGRITIDDIVDVIKEEADKDYQLAAGISQDVEADD
                     SIIELTKARLPWLVLALCGGFISVEMLGLFGGAMEKHGELFFFTPLIAAMAGNVGVQS
                     SAIIVQGLANQSISGSIVNRLLKEISLALLNGVILATILFIGSHFILGVDPKIGMIVT
                     IALISVIIIASLIGTFIPLILNKFGIDPALATGPFITTSNDICGILIYFSIAKIILGF
                     "
     gene            complement(18839..19615)
                     /gene="rsmA"
                     /locus_tag="PK218_02590"
     CDS             complement(18839..19615)
                     /gene="rsmA"
                     /locus_tag="PK218_02590"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPW97428.1"
                     /translation="MQVKAKKHLGQHFLTDESIAKNIADTLSFSGYETVLEIGPGMGV
                     LTKYILEKEIDTYVIEIDTESVSYLDSHYPKLHNKIISKDFLKYNINEVLEGKPFAII
                     GNFPYNISTQIVFKCLEMRNQVPEFSGMFQKEVAERICSKKGNKVYGILSVLSQAFYD
                     VEYLFTVEPEVFNPPPKVKSGVMRMRRKEDFSLPCDEKLFFNVVKTGFNQRRKTLRNS
                     LKSFQLSDNLKEDTIFDLRPEQLSVEQFVEITQKIAANAV"
     gene            complement(19649..19960)
                     /locus_tag="PK218_02595"
     CDS             complement(19649..19960)
                     /locus_tag="PK218_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HPW97429.1"
                     /translation="MIIYNVTINIHESVHDQWMIWMHHKHIHDVLNTGKFTSARMVRV
                     LVDEEMGGMTYAVQFTTDCKETLQKYYDEDAPKLREEGMQLFGEKMLAFRTELELISE
                     Y"
     gene            complement(20103..21881)
                     /locus_tag="PK218_02600"
     CDS             complement(20103..21881)
                     /locus_tag="PK218_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW97430.1"
                     /translation="MKKFFIVLFLFLTICAKAQNEQLANNYFDRGEFEKALVSYEELL
                     KMQVGNSNYFQRVVECYQQLEQYDKAEKALQERLDKYRQSNLLVELGYNYQLQKNNEK
                     ATKYYDQAIEKIKINANEVYGIAYVFERKSLIDYALLAYQTALEKEPKMSFNFQMAVL
                     YGQKGNTDLMIETFLAEGFQNPQNLPIIQNQLSRFMTEDTDENFNNTLRKALLLRTQK
                     SQDLFWNDFLSWYYVQLKEYSKAFIQQKAIYKRNPESFSNIVNLAEMAIDDKDSEAAK
                     EILIFVLDNTNNLDLLIQAHSYLVEMKIDIATEKDYPAITQELDVLIKQFGVSPYTLS
                     LLKLDANFSAFHLKDTEKAKTILKNAMDMPLNKYQLAEVKMDLADILLFEEKFNQALI
                     YYSQIENDLSGDVIGQEASLKTAKTSYYKADFKWASHQLKVLKSASTQLIANDALDLF
                     LLISDNTVEDSTQVALKQFSRGDFLLYQNKKQDALSQFQLILKEHKGEEIEAVTLLRI
                     GKIYEKQGDYTKALENYNEIITNHKDGIYIDEALYYSAEIYNLKLNDAEKAKPLYEEM
                     IFKHEDSIYYVDSRKKYRQLRGDTTL"
     gene            complement(21961..23232)
                     /gene="serS"
                     /locus_tag="PK218_02605"
     CDS             complement(21961..23232)
                     /gene="serS"
                     /locus_tag="PK218_02605"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPW97431.1"
                     /translation="MLQISYIRENKDKVITALAKKHLDAKTIIEEVIELDENRRSTQN
                     DLDNTLAEANKLSSAIGEMMKNGEKAKAEILKLKTSQLKETSKDLSEKVDGFAAELLQ
                     RMYLLPNLPAEIVPVGKTPEENLNVFEEGYIPVLHENAMPHWDLVKKYDIIDFELGVK
                     ITGAGFPVYKGKGAKLQRALIAYFLDKNTEAGYQEVQVPHLVNEASGYGTGQLPDKEG
                     QMYHVGIDDLYLIPTAEVPVTNLFRDVILQENELPVLYTGYTPCFRREAGSYGAHVRG
                     LNRLHQFDKVEIVRIEHPEKSYEALDGMVEHVKNIMRELKLPFRILRLCGGDMSFASA
                     LTYDFEVFSTAQDRWLEISSVSNFETFQANRLKLRFKGKDGKTQLAHTLNGSSLALPR
                     VLAGILENYQTPEGIVIPEVLRPYTGFDIIN"
     gene            23409..25328
                     /locus_tag="PK218_02610"
     CDS             23409..25328
                     /locus_tag="PK218_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPW97432.1"
                     /translation="MKNIYLFFFCISVSFVFSQKKESTTKTIAEAEMNSASRIANFQA
                     NPNTANYDVTYQKLEFTVNPAVKFVSGKITTTFTALTNMNSITFDFANELTASVIKKG
                     ATNLSFTENSNNELVITLPSTQLAGTSATIEVTYSGVPPNNGFDSFVKSTHNGTPIIW
                     TLSEPFGARDWWPCKQDLNDKVDSIDVYITAPSQYIGVSNGIEPEAPVINGANKTTHF
                     HHGYPIPAYLIAIAVTNYTVYNQVGGTAPNEYPIINYIYPENFASIQPQLSETPAILE
                     LYENLFEIYPFHNEKYGHAQFGWGGGMEHTTVSFMVGFDRQLIAHEMGHQWFGDKITC
                     GSWKDIWLNEGFATYLAALVIENFDGLDSFIAEKIDMVDYITSSPNGNVYLDDVEATN
                     VNRIFSSRLSYNKGAMILEMLRFKMGDTAFFQALRNYLADPNLAYKYAVTTDLKSHLE
                     AVYGQDLTEFFDDWVYNEGYPTYDITAQNWGSGQAKFVVSQTQSDASVSYFEMPVPVR
                     VYGSNGEEQDIVLENTFDNQEFIVNVPFTITNVVFDPEQHLIARNSTATLQNSNVEWN
                     QTISVFPNPTNSILHIQKPNDLSIESVTLYNSLGQMVLKSNENDLTIESLSNGVYELQ
                     LNTNQGKFHKRIIKQ"
     gene            25411..26757
                     /gene="rseP"
                     /locus_tag="PK218_02615"
     CDS             25411..26757
                     /gene="rseP"
                     /locus_tag="PK218_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPW97433.1"
                     /translation="MEIVIKLSQFLLSLSILIILHELGHFIPAKLFKTRVEKFYLFFD
                     VKYSLLKKKIGETEYGIGWLPLGGYVKIAGMIDESMDKEQMQGEAQPWEFRSKPAWQR
                     LIIMLGGVTVNFILAFIIYIGMTYVYGDYYIKNNDLKDGVWVTNPVVEKAGIKTGDRI
                     VSVDGTPVERFVPSVSLDILMSKKIIVERNGQNVEIDLPVNFIGKILDENKKEVIRLR
                     IPFVVSSISDESANKDVLMAKDILVSVGGTSAKYYDEALKYFEEHKGQKVAAVVLRDN
                     KEVAVSLQINNEGKLGVISGGLKEDNLEKLGYYKFSKESYGFLESFPIGIDKGVTQLS
                     NYWKQLKAIFNPNTGAYKGVGGFAAIYNVFPDTWSWETFWNITALLSIMLGVMNLLPI
                     PALDGGHVMFLLYEMITGKTPSDKFMERAQLVGFVLLISLLLFANGNDIYKAIVGK"
     gene            26841..26914
                     /locus_tag="PK218_02620"
     tRNA            26841..26914
                     /locus_tag="PK218_02620"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26875..26877,aa:Asn,seq:gtt)
     gene            complement(27029..27108)
                     /locus_tag="PK218_02625"
     tRNA            complement(27029..27108)
                     /locus_tag="PK218_02625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27072..27074),aa:Leu,seq:caa)
     gene            27400..28341
                     /locus_tag="PK218_02630"
     CDS             27400..28341
                     /locus_tag="PK218_02630"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPW97434.1"
                     /translation="MSHQEPEAKIFACSQSVHLAEEIAKNYGIPLGKVTFSKFSDGEF
                     QPSFEESIRGLRVFIVCSTFPSSDNLMELLLMIDAAKRASARHITPVIPYFGYARQDR
                     KDKPRVPIGAKLVAKLLETAGATRIMTMDLHADQIQGFFERPVDHLFASTIFLPYVKS
                     LQLDNLTIASPDMGGSKRAYAYSKFLDSDVVICYKQRKAANVIGTMELIGEVKGRNVV
                     LVDDMVDTGGTLAKAADLMIEKGALSVRAICTHAILSGDAYEKLENSQLSELIVTDSI
                     PLKRESNKIKVVSCAPLFAEVMHMVQHNNSISGKFLM"
     gene            28407..29015
                     /locus_tag="PK218_02635"
     CDS             28407..29015
                     /locus_tag="PK218_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPW97435.1"
                     /translation="MKSITIKGSERESVGKKATKALRDAGMVPCVIYGGNQPVHFAAE
                     EKAFKSLVYTPNAHTVVVDLAGKTFNAILQDIQFHPVSDRINHIDFFQLNDEKEIMME
                     VPVKITGTSPGVLLGGELRLNQRRLKVKALPKNLPDYVEANISELQMGNKLYVTKLVL
                     GDFKLMHPDNTVVAQVKISRAAMKAAQEAAKAEKTAAKGKKK"
     gene            29150..29716
                     /gene="pth"
                     /locus_tag="PK218_02640"
     CDS             29150..29716
                     /gene="pth"
                     /locus_tag="PK218_02640"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPW97436.1"
                     /translation="MKKFLIVGLGNIGSEYTNTRHNIGFKILDYFASKENCSFQTMKL
                     GDIAEYSIKGRKLIFLKPNTYMNLSGKAVKYWMEKENIEKENILVITDDLNLSFGSIR
                     IKAKGSDGGHNGLKNIQLLLNTTEYPRFRFGISDQFKKGQQVDYVLGEWNEDENSKLT
                     ERLDLSIKIIQSFALAGLNNTMNEFNGK"
     gene            complement(29760..30995)
                     /locus_tag="PK218_02645"
     CDS             complement(29760..30995)
                     /locus_tag="PK218_02645"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HPW97437.1"
                     /translation="MKKIIIVFLFVNSIVAIAQRTCHTNDKMNELIQNNPEFKIHHEE
                     TMDFIRNPNNTNAALNRSTSVVYTIPVVVHVLYKNAAQNVSEAQILSQIGILNADYRK
                     TNTDFNSVVPTAFRPLGADFELAFCMATQKPDGTATNGIERKSVASSFVFENQYYTSA
                     GLPVWDPTKYLNIWVGRFTDNTLLGFAYLPSIVGTSYANRDGLCIGDKFFGNTGTAQA
                     PYNKGRTATHEIGHYFGLDHPWGDGNCDADDGVADTPATYDAYYDCVSFPNNTYMCSP
                     STNGAMFMNYMDYVPDACMAFFTAGQKAIAQATMNGPRASLKTSNGCQSLNVTEQEAI
                     QSIAIYPNPVSQFFMITSPQINVDYVEIFSTNGQMVKSQKLDEVNNKIYVDNLETGVY
                     YLRIYNENRLLKSDKLIKK"
     gene            31230..31748
                     /locus_tag="PK218_02650"
     CDS             31230..31748
                     /locus_tag="PK218_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97438.1"
                     /translation="MKKIFLIPLAFSLSFCNCKKSVVENSKTTSIESVCPDNGICTVK
                     IYKNKSLEVKTDDLGGIYYQMLDNTATSVIQYQYNKTVEEGLQDGQHREEILFEINSS
                     KPLLNLKDKNLQEVKMIFGRHCFCKGQAGYFKVEEGSLSLENKNETTTFELDFKIHKV
                     PQLFEKVKATVQ"
     gene            complement(31801..34485)
                     /locus_tag="PK218_02655"
     CDS             complement(31801..34485)
                     /locus_tag="PK218_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HPW97439.1"
                     /translation="MKKNLLLFVIAFAFFASSYGQSSLWVKASNERVSALPKMDRATI
                     PNEYDLYALNFEALKNQLQNAPLDSSTTKSNVVIAFPNSNGQIQNYRIYEAPVMEKGL
                     SDKFPEIKSYSGKGVDDASASIRFSTTLFGLHVMSLSGKSGTFYIDTYTKDLNYYIVY
                     NKKSIHKQENKFNCLVQDETAFVDANTNNLQERLVNDGTFRQYRLAMACTIEYAAFHV
                     AAAGLNSGTLAQKKAAVLSAMVVTMTRVNGLYERDMSLRMNLVANNDIIIFITSDNFT
                     NNNANALINESQTVIDGAIGTANYDIGHTVSTGGGGLAGLGVPCSSSKARGITGSDSP
                     VGDPYDIDYVAHEMGHQFGATHTFNNSCGGNISTTTSVEPGSGTTIMGYAGICAPDIQ
                     GNSDAHFHAVSIAQMTSFVVNNGTCSGNTLNGNTAPVVDAGLNYVIPNGTAFILKGSA
                     TDANGDALTYCWEQTNTQVSTQPPLATSSTGPNFRSLPPSTSPNRYMPSFADVLNGNL
                     TPTWEVVPSVARTMSFALTVRDNRMPNGGQTNRDNMNVVFANVGPFKITSPNVENVSW
                     NQGSTQTITWDVAGTTANGINTSNVNILLSTDGGVTFPTVLAANTLNDGSESVTLPFV
                     ASTTCRIMIEAVGNIFYTVSKNIALGYTIVNECNTYNFTGGPISIVDQAPGNYTIRTL
                     VIPATGTISDVNVSNSITHTYLSDVVTEISSPANPTTFVKLFDRGCGNASATLNMKFS
                     DGSPGINCSGGNTLQTVSPSGSLATFNGQNPQGTWTFRVYDNFAGDSGTVNSWSVEVC
                     TQTVTLSTPENNIGFNDFALYPNPNNGNFTIQFSSNSTNDVNVMVHDIRGREIMKQVF
                     ENKPLFNENIQLKNVQKGIYLVTVSDGVSKQVKKIAVE"
     gene            34687..35628
                     /locus_tag="PK218_02660"
     CDS             34687..35628
                     /locus_tag="PK218_02660"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPW97440.1"
                     /translation="MKLFHSISDFKCKSKTIVTIGTFDGVHLGHQKILEKITSNASLS
                     NLESLVLTFFPHPRTVLQEGTEMKQLNTIEEKINLLDKIGIDNLVIHPFDKEFSRLTA
                     EDFVKIVLVDKFHASKIIIGFDHRFGRNRTADINDLIDFGEKYQFDVEQISVEEINDV
                     SVSSTKIRNALNEGDVTLANTFLDYRYSIKGLVIKGKQLGRTIGFPTANIQINEVYKL
                     IPKEGVYIVCCDVESSTYYGMMNIGLNPTTDANNQKQKIEVHLFDFNEDIYEKPITVS
                     FLKRIRDEQKFESVAALKNQLSLDKLNAISYIEKTLS"
     gene            35742..36176
                     /locus_tag="PK218_02665"
     CDS             35742..36176
                     /locus_tag="PK218_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97441.1"
                     /translation="MKLSKELVNGFLIFAGIAIFFLAMEIAGLANNFYLRFFNVLFVF
                     YGVNRTVKQNIAEGKNGYLKNMFSAGLTAFIGVILSIISLVAFIHYKGGDAYIANLSE
                     KFLFGGASVNEYSIGLLFEGLASGMIIVFITLQVWRGKTVFK"
     gene            36256..37341
                     /gene="bioB"
                     /locus_tag="PK218_02670"
     CDS             36256..37341
                     /gene="bioB"
                     /locus_tag="PK218_02670"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HPW97442.1"
                     /translation="MSITKHNWTKEEIIAIYNKPMMDLLYEAASIHREHHDPNVVQVS
                     TLLSIKTGGCPEDCGYCPQAARYHTKVEGNDLMSVSQVKAQALRAKESGSSRVCMGAA
                     WRNVKDGEEFDQVLEMVRTINKLDMEVCCTLGMITENQAQRLAEAGLYAYNHNLDTSE
                     DYYKEVISTRAFDDRIQTIENVRKTNVTVCSGGIIGMGESIEDRAGMLVALSTLNPQP
                     ESVPINALVAVEGTPMENEKPVEIWEMIRMVATTRIIMPETQVRLSAGRTEMSREGQA
                     MCFFAGANSIFAGDKLLTTPNPDVNEDMKMFETLGLIPQKPFVKVMQPTTVEAEDSQF
                     TPLGEKPRWTRPGHTIERNLEASGKGK"
     gene            complement(37399..>38715)
                     /locus_tag="PK218_02675"
     CDS             complement(37399..>38715)
                     /locus_tag="PK218_02675"
                     /inference="COORDINATES: protein motif:HMM:NF033708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS sorting signal type C domain-containing
                     protein"
                     /protein_id="HPW97443.1"
                     /translation="GTFGNWRVPTTPTMTEGRGYIARTFNGSATATTLPFTFYGQPNN
                     GLITVPIARGNYFGDGITTGLNYDAEPANPNNVDTTRWDDNWNLVGNPYPSAVNAIKF
                     LTANTNLEGFINIWTHLTPPASTVDPYYYNFGYNYTPADYITYNLSGAVTGPGAFNGN
                     IASGQGFFVLINDGAATTGTAFFNNAMRSDITNKLNYDNSQFFRTSNFGDAEDEKNRI
                     WLDIIAPNNQTDRTLVGYVTNASNNTDRLYDAVTKATGLKLYSFSNDVPLQPLCIQGK
                     TLPFDNQDTIRLGYNSNSVGNYKIAIAAVDGLFANNQAIYIEDMLLGIIHDLRDAPYT
                     FTTTKGMFEDRFILRYTTQALGNANFDSTNQVVISTNHSQINIISNTETISQVQIFDL
                     LGREVYTMSNLDTLMYSISNNVRNQALIIKITLTNGVLVSKKIIIN"
BASE COUNT        12786 a         6164 c         6604 g        13161 t
ORIGIN      
        1 tccattcgta gtgttatttg ctgtaccatt atcatttatt ggagctttat tagctttagc
       61 gttaaccaat aattcgctta atattttcac cattcttgga atcattatgt taattggatt
      121 ggtttgtaaa aatgccatta tgttggtaga ttatactaat caaagaagag cagcaggaga
      181 atcgataaga actgccttga ttcaagcaaa tcatgccaga ttacgtccaa ttttaatgac
      241 aactattgcc atggtttttg gtatgttgcc aattgccttg gcttctggtg ctggtgctga
      301 atggaaaaat ggtttagctt gggtaattat tggtggatta atttcttcct tattcctaac
      361 cttaattgta gttccagtaa tatatgaaat aatggaaaaa atagttggaa aattttctaa
      421 aggtgaaaaa attgattacg aaaaagaaat ggttgccgat tatgttcatc aagaattaag
      481 tgatgatgga tttaatccaa aacatacctt ataaacttta agggttttaa aaaaaagtct
      541 cgaattatcg agactttttt ttaattataa cttttttatt atttatttac tatggcttct
      601 tgagtccaag cttttacatt ggatatttta ctttttgaaa aatctacttc gcttaatgat
      661 ttatcattcc aaaaaaacca ttttcctgtt ttttctccat ttgcatattc accaattgct
      721 tttttatttc cactttcatc ataagaaacc cattgaccat ttaactttcc atctttaaaa
      781 aacccttctt gttgaatttg tccgttttcg tagtaatatg ttgctttaac taaatctcca
      841 ttttgttcta aaactggctt aacttcttgg gcaaaagtat atccagttat taaaaatgct
      901 gctattatca ttgactttct catatcgtag gttttgttta tttataatta cataacaaat
      961 atataattta ttttacaata aaaaaacttt cacttaacaa tttggtaaca ttgaaattga
     1021 atttaacttt tctacaacaa aaaaccacat ttaaaaatgt ggttattgtt ttattgatta
     1081 atttatttta ctgcatcttt attttaataa tccttccaat tgttctttca attttgggtt
     1141 agatgggcgc ttagcatctg catcaactac ttttccagtt ggatcaatta aaatgaatct
     1201 aggaatagaa ttaattccga acgcttttat aaaatcagaa ctccagttat tatctgcaat
     1261 taattgagtg ccgccaagcg atttgtcttt gacaaaatct ttccattttt cataatcttt
     1321 ttcaacatct accgaaatac taacaaactc aatatttttc ccttgcatac tttcttccaa
     1381 agtttttaaa gatggaattt cagctctaca tggtccacac caagttgccc aaacatcaat
     1441 ataaacgtat ttccccttta agctctctaa agaagttttt ccaccagcat gattttcata
     1501 aacaaaatta ggagattgaa tattgtttaa ttttttattg gcagaaatct gattgtaata
     1561 ttttgtcaat ccagccaaat tcatttcagc acttttcttt aacacttcaa gcaattttac
     1621 atccaaacca gctgcttcaa attttttatt gtcggttgtc cttttctctt caacagcttt
     1681 tttgaaattt tcttcatcca tagttagcac ttggtcataa ggatatttct gttcttccat
     1741 agcttgttta gccaaaaagt tgttctcttt agaaccttcc ccatcaaact ttatgctttc
     1801 atcaaacatt ttaccatcta atgtcatttt taaattataa ccgtttttta aatacaattg
     1861 cgcatattca acaccatcaa acagttgata taacccttct tttattggaa atgtagcttt
     1921 aaaagcacct ttgtcgtcca cttttatttc cttaacaatt actctgtttt cttttataaa
     1981 caagacatca ccattcttat ttataacatt tgcttcaaaa gtcatggttt cttgggcaaa
     2041 agaaaatgta accattaaaa caagaaacaa ggaacttaca atttttttca ttttataaat
     2101 ttttaaattt caaactcaaa tataattttt ttcttgggga taaagtgcaa tttaacaaaa
     2161 tattctacct tatatattga tttaagtttt ttacttttgc aaaaaattat tctaatgttc
     2221 agaagtcaca ataatggcga attaaacgct acacacatta ctaaagaagt tacacttgct
     2281 ggttgggttc aaaaatcacg tgacaaagga tttatgattt gggttgattt acgcgaccgg
     2341 tatggtatta cacagttaat ttttgacgaa caacgcacag ataaaactgt ttttgaaaaa
     2401 gcaaaatcac ttggaagaga gtttgtaatt caagttaaag gaacggttat tgaacgtgag
     2461 tcaaaaaatc taaatatgcc tacgggtgac atagaaattt tggtttctga acttattgtt
     2521 ttgaatgaag ctttacttcc tccatttaca attgaggatg aaacagatgg tggtgaagac
     2581 attagaatga aattccgtta cctagatatt cgaagaaatc ctgtaaaaaa tagtttgctt
     2641 ttccgtcaca aagtagcaat ggaagttcgt aaatacttat ccgatttaga tttttgtgaa
     2701 gtagaaacac cttatttgat taagtcaact ccagaaggtg caagagattt tgttgtacct
     2761 agcagaatga atgaaggaca attttatgcg ttacctcaat cgccacaaac gtttaaacaa
     2821 ctattgatgg ttggcggaat ggataaatat tttcaaatcg taaaatgttt ccgcgatgaa
     2881 gatttacgtg ctgatagaca accagagttt acacaaatcg attgcgaaat ggcattcgta
     2941 gaacaagaag atattttaac tgtttttgaa ggattgacac gtcatttatt aaaaaaatta
     3001 aaaaatgttg aagttgccga gtttccacgt atgacctatg attatgcaat gaaaacgtac
     3061 ggaaatgaca agcccgacat tcgttttgat atgaaatttg gcgaattaaa tcaagtagca
     3121 caacacaaag attttggcgt tttcaattct gcagaattag tagttggaat tgcagctccg
     3181 ggatgtgcat cttacaccag aaaagaaata gatgcgttaa ttgattgggt aaaacgtcct
     3241 caagttggtg caagcggaat ggtttatgta aaatgtgaag aagacggcac atttaaatct
     3301 tcggttgaca aattttacaa tcaagaagat ttaaaaaaat gggcagaaat aaccaatgca
     3361 aaatcgggtg atttaatttt agttttatct ggaaatgcag acaaaaccag aacccaactt
     3421 tctgctttac gaatggaagt tgcaacacgt ttaggattaa gaaatcctac agaatttgcg
     3481 ccactttggg ttatagattt tcctttacta gaatgggatg aagaaagtgg tcgttatcat
     3541 gctatgcacc atccgtttac atctccaaaa cccgaagaca tgagtttgat tgcaaccgaa
     3601 cctggaaaag ttcgagccaa tgcctatgac atggtattaa atggtaacga aattggcggt
     3661 ggttctatta gaattcatga caaagattta caaagcagaa tgtttgattt attagggttt
     3721 tctaaagaag aagcccaaaa tcaatttggc tttttaatga atgcttttga atttggagca
     3781 cctccacacg gcggattggc atttggtttg gatagattag tagccatcct tggaggtcaa
     3841 gaaactattc gcgactttat tgcttttcca aaaaacaatt ctggccgtga tgtaatgatt
     3901 gatgctccta gtgctataga caataaacag ctagaagaac tgcatattaa attaaagtaa
     3961 ttttttatac gctaaatttt aatctttaag ggtattatct tgatgaattt gcaattattt
     4021 aacattgaaa tagttgtaat ctcacaaata aatttatctt tgaacattat taattatttt
     4081 tttttacaaa atgcgaacag gcaaagttaa atttttcaat gagtcaaaag gttacgggtt
     4141 tattacagac gacgaaactg gcaatgacat ttttgttcac gcttcgggcg ttcaaaacaa
     4201 cacacttaac gaaggagaca aagtttctta cgaagaagaa gaaggaagaa aaggtaaaat
     4261 agcggcacaa gttaccccta tcgaagacta ataaatatta ttttttgaat gcctagaatg
     4321 aattaacgtc tcgaaatatt tcgaggcgtt tttatttata attaatttaa ataagcgttt
     4381 ttttagacag ttataacaaa cgtttatgta ttgtttcttg cgttttaatt attgcgatgt
     4441 atatttgtaa agttttaatt tttaatccaa attaaggata tgcaatccaa tcaattagat
     4501 tatttgccaa tagtaatgca agctggttta gctataggtt ttgtggtatt aactatcatt
     4561 ggctcaagtt ttttgggtcc aaaaagacat tcaaaaaata aagacaaaaa tttcgaatgc
     4621 ggaattgaat cagtcggaaa tgctagaata ccgttttcgg taaaatactt tgttgtagct
     4681 attctatttg tattattcga tgtagaagtt atctttcttt atccttgggc aattaacttc
     4741 aaggaattag gcatggaagg aatgattaag atggtaatct ttatggcact tttattaatt
     4801 ggatttttct acatcattaa aaagaaagcc ttagaatggg aataaatcaa taattgctat
     4861 gagtacttca aatccaaata tggttgctcc accagaagga gttgtaggcg aaggttactt
     4921 tgctacaaaa ttaaacgatg ttgtcggatt agctcgtgct aattcgcttt ggccattgcc
     4981 ttttgctact tcatgttgcg gaattgaatt tatggctacc atggcatctc attatgattt
     5041 ggcgcgtttt ggctccgaaa gagtaagttt ttcacctcgt caggctgata tgctaatggt
     5101 tatgggaact atttcaaaaa aaatggcacc tatacttcgt caagtttatg aacaaatgtc
     5161 tgaacctaga tgggtaattg ctgttggtgc ttgtgctagt tcaggtggtg tttttgacac
     5221 gtattctgta ttacaaggaa ttgacaaagt aatcccagtc gatgtttacg ttccaggttg
     5281 tcctccaaga ccagaacaaa ttgtagatgg tgtaatgcag ttgcaagaat tagttaaaaa
     5341 cgaatctgtt cgcagaagaa attcaccaga atacaaagaa cttttagctt cttataactt
     5401 ataatatggc attagaaaca acgtatatac aagaaaaatt atctcaaaaa tttggcagta
     5461 atgtgcttaa ttttgaaatg aaaagagata tattttcttt ggaagctact tcagaaaccg
     5521 ttcatgaagt aatccaaact ttaaaagaag acgaaagttt gaattttcat tttctaacgg
     5581 acgtatgtgg aattcacttt ccagacaatg acgaaaatca ccaatttgct gtggtatatc
     5641 acttgcacaa ttggattgaa aatatgagaa ttagagtaac tactttttta aaaggtgaaa
     5701 atcctgaagt ttcatcaatt atagatttat ttacttctgc caattggatg gaacgtgaaa
     5761 catgggattt ttatggaata aatttcatcg gtcacccaca attaaagcgg attttaaata
     5821 tggatgaaat ggtttctttt ccaatgcgaa aagaatttcc tttagaagac agtggaagaa
     5881 ccgacaaaga cgatcgtttt tttggaagaa caatcaaaca aataaaataa ataattcaac
     5941 attataaaat gtcagaacta ttattaccac cagagcatcg atatgccaag ataattgagg
     6001 aaactcgcaa tagtgacgga agtgagctta ctattttaaa cttaggtcca acacatccgg
     6061 ctactcacgg tatttttcaa aatatccttt tgatggatgg cgaaagaatt attgatgccg
     6121 aaccaacaat aggatacatt cacagagctt ttgagaaaat tgccgaaaat cgtccgtttt
     6181 atcaaattaa tgttctaacc gatagaatga attattgttc ctcgccaatc aacaatatgg
     6241 cttggtggat gactgtcgaa aaattattaa aaatagaagt tccaaaacgc gctcaatatc
     6301 ttagagttat cgtaatggaa ttggcgcgta taacagatca tttaatatgt aattccattc
     6361 ttggtgttga tactggagcc tatacaggat tcctttatgt attccaattc cgtgagaaag
     6421 tttacgaaat atatgaagaa atttgtggtg ctcgattgac tacaaatatg ggaagaattg
     6481 gtggttttga aagagattgg tctgaggaag cttttagaaa actagatgtc tttttaactg
     6541 attttccagt agcatggaaa gaatttgaaa acctatttga aagaaataga atttttatag
     6601 ataggacagt aaatgtaggc ccattaacag ctgaaagtgc tatggattat gggtttacag
     6661 gaccaaatct tcgtgctgca ggaattgatt acgacgttag aaaagcacaa ccctattcat
     6721 cgtatgaaga ttttgaattt gatgttcctg ttggaaaatc tggtgatact tatgaccgtt
     6781 tttgtgttcg taatgcagaa gtttgggaaa gtttaaaaat cattcgtcaa gctttagaca
     6841 aattaccaga aggtccaatt catgctgatg ttccggatta ttatttgcca ccaaaagaag
     6901 aggtttataa caacatggaa gcattaattt atcactttaa aattgtgatg ggtgaagttc
     6961 cagttcctgt tgatgaaatt tatcatgccg tagaaggagg aaatggagaa ttaggatttt
     7021 atttaataac agacggaagt agaacacctt atcgtttgca ttttagaaga ccatgtttca
     7081 tctattatca agcctatcct gaaatgatta aaggttcaat gctttcggat gctattgtaa
     7141 ttttatcaag cttaaatgtt attgcaggcg aattagacgc ataaaaacag tgtagaattc
     7201 agattttaga ctatcgattg aaaaatctaa tatctaaaat ttaaaatcta aaattagaaa
     7261 tggaaatatc acacataaaa caagaaataa acataactga atcgttgatg acacggatta
     7321 atgaattaat cagtcattat cctgcagata aaagaaaatc agctttatta cctgttttac
     7381 atgaagttca agatgcacat gacaattggt taagcatcga tttacaaaat aaagtagccg
     7441 aaattcttga aattcaaaac attgaagtct atgaagttgt tactttttat acaatgttta
     7501 accaacgccc aattggaaaa tacatgtttg aattctgtca aacttctcca tgttgtcaaa
     7561 atggggttga agatttaatg gattatactt gtgaaaaact gggcgtaaaa gtaggcgaac
     7621 caacgaaaga tggaaatttt gaagttcgtg gagttgaatg tttaggtgca tgtggatatg
     7681 cgccaatgat gcaactaggt gatttttatc aagagcattt gacaaaagaa aaaatagatc
     7741 agttaatttc ggattgcaaa gaaggaaaaa tcactttaca cgataaataa tatcatggga
     7801 agaaaaatat tattagacaa aataaacgtt ccaggcatta aaacctacga ggtatatcgc
     7861 aaagaaggcg gttatgcatc tgtagaaaaa gctttgaaaa aaatgactcc agatgaagta
     7921 gttgaagaag taaaaacttc aggacttcgt ggtcgtggtg gtgcaggatt tccagctgga
     7981 atgaaatgga gtttcattga taaaaaatca gggaaaccac gtcatttagt atgtaatgcc
     8041 gacgaatctg aaccaggaac tttcaaagac aggtatttaa tggaatacat tcctcactta
     8101 ttaattgaag gaatgattac ttcaagttat gcacttggag caaatttatc ctacatatat
     8161 attcgtggag aatacatgtg ggtttataaa attttagaaa gagcaatagc cgaagctaaa
     8221 gcagctggtt ggttagggaa aaatatttta ggttcaggtt acgatttgga attgtatgtt
     8281 caaattggtg gcggcgctta catttgtggt gaagaaacag cacttattga aagtttagaa
     8341 ggaaaacgtg gaaatcctag aatcaaacca ccatttccag ctgttgaagg tttatggcaa
     8401 aatccaactg ttgtaaacaa tgtcgaaact attgctgctg tgccttggat tgtaaataat
     8461 tcaggtgctg attatgctgc aatcggcatt ggtcgttcaa caggaaccaa attaatttct
     8521 gcttctggaa acataaaaaa ccaaggcgtt tttgaaattg atttaggatt atctgtttac
     8581 gaattcatga attccgacga atattgtggc gggatgcaaa ccgacagacc attaaaagca
     8641 ttagtgccag gaggaagttc tgtaccaata ttaccagcca acttaattta caaaactgca
     8701 gctggcgaag atagattaat gtcttacgaa agtttgtcag atggcggatt tgcaacaggc
     8761 tccatgttag gttcaggcgg atttatcgtt tacgatgaaa gagcttgtat tgtaagaaat
     8821 acatggaatt ttgcacgctt ttaccaccac gaaagttgtg gacaatgcac accatgtcgc
     8881 gaaggaacag gttggttaga aaaagtttta tggagaatag aaaacggtca aggtcgcgaa
     8941 gaagacattg atttattatg gagtattcaa gcaaaaatag aaggaaacac catttgtcca
     9001 ttgggcgatg cagcttcatg gccagttgct gcggcaattc gtcattttag agatgaattt
     9061 gaataccatg ttcgttttcc agaaaaaatt aaaaatagag atcactttgt aaatgagcca
     9121 tttgaaaaag tgaagcattt agtagcaaag caaacagtat aatttaaaag tctgaagttg
     9181 gaagttggaa gttggaagtg gcaacactta accttttaaa cttttaaact tttaaacaaa
     9241 gaaataatga aagtaacgat agacggacaa gaaattgaag tagctccagg aacaacaatt
     9301 ctgcaagcgg ctcgtatgat tggtggagaa tctgttccgc cagccatgtg ttattattca
     9361 aaattaaaag gaactggcgg aaaatgccgt tgttgtttgg ttgatgttac caaaggtagc
     9421 gataatgatc cacgtccgat gccaaaatta atggcttctt gtgtaacggg ttgccaagac
     9481 ggaatggaaa ttgccagcaa atcatccgaa agagttaaag aagcaagaaa tgcagtaact
     9541 gaattccttt taataaatca tcctttagat tgcccagttt gtgaccaagc aggtgaatgt
     9601 gatttgcaaa atttaagttt cgaacatggc aaattacaac accgttttat agaagaaaaa
     9661 agaacttttg agccagaaga tattggcgat aaaattcaat tgcacatgaa ccgttgcatt
     9721 gtttgtcagc gttgtgtaga agttgcagac caattaactg acggaagagt tcacggcgtt
     9781 ttaaatcgtg gagaccattc tcaaatttca acttgtgttt ctgcagctat cgataacgaa
     9841 ttctcaggaa acatgattga tgtttgtcct gttggtgctt taaccgacaa aacattccgt
     9901 ttcaaatcga gagtttggtt taacaaacca tttaatgctc acagagattg cgacaaatgt
     9961 tgtggcaaaa ctacactttg gatgtttgga aacgaaattc aaagagtaac cgctagaaaa
    10021 gatgaattcc atgaagtaga agagttcatt tgtaatacgt gtcgttttga taaaaaagaa
    10081 gtttcagatt ggactatcga aggtccgaga aaatttgaaa aagattcggt tatcaatcaa
    10141 aacaattata ctcgaaaaat ggataaagtc gttatcaata ccgaagaagg aattcttgct
    10201 ggaagagcaa ttgaccgtaa aaaaatcagt atggctgaga tagattacaa cgagaaaatt
    10261 gatggtacta atataaattc gaataaaaat ggatagtgct ttcatcattg aaaaaagtat
    10321 ttttatagta gtgatttttg ctgtcactat ggttatggcg atgtattcta cttgggcaga
    10381 acgtaaagta gcggcttatc ttcaagatag agttggtcca aaccgtgcag gttggggtgg
    10441 tttattacaa ccgcttgcag acggaatgaa attgtttgcc aaagaagaat ttgaacccaa
    10501 tacaccaaat aaatttttat tctacgttgg tcctgcaatt gcaatgagca cggctttaat
    10561 gactagtgcc gtaattcctt ggggtgataa attagagatt tttggaagaa ctgttattct
    10621 tcaagctact gatatagaca atgcattact ttatgttttt gctattattt ctatcggaat
    10681 ttatggaatt atgattggtg gttgggcatc aaataataag ttttctttaa tgtctgccgt
    10741 tcgcgctggt tctcaaatgg tttcttatga aattgcaatg ggattgtcta ttattgcttt
    10801 attaatgatg acaggaacat tgagtttgag agaaatgtca atgcaacaag caggaatgaa
    10861 ttggaatgtt ttttatcaac cggttggatt ccttatattt ttaatttgtg cctttgccga
    10921 aactaaccgt actccgtttg acttggcaga atgtgaatcg gagcttattg gaggttatca
    10981 taccgaatat tcatcgatga aaatgggatt ctatttgttt gctgaatatg ccaatatgtt
    11041 tatttcatca actattttag ccgtgttgta ttttggtggt tataattatc ctggcatgca
    11101 gtggatgaca gaacatgctg gagttaacat agcaaacatt cttggaatta ttgttttatt
    11161 catcaaaata tgtggattta ttttcttcta catgtgggtt cgatggacaa ttccaagatt
    11221 tagatacgat caattaatgc atttaggatg gaaaatatta attccgttat ccatcatcaa
    11281 tattgtgatt acgggtattt gccttttagc ttttaattaa gagaaaaaaa ttatgtcaac
    11341 atcaatacaa aatatatcct tatcgggtca caaaaaacaa gtttctaata aagaaatgac
    11401 tttttgggag cgattatatt tgatagccat tatcaaagga ttattaatta ctattaaaca
    11461 ctttttcaaa aagaaagcaa ccattcatta tcctgaacaa gtacgagaat ttagtccagt
    11521 ttatagagga aaacacactt tgaaacgtga tgaattgggt agagaaaatt gcaccgcttg
    11581 tggtttatgc gcactttctt gcccagcaga agcaattacc atgaaagcag cagaacgcaa
    11641 aaaaggcgaa gaaatgcttt atcgtgaaga aaaatatgcc gaaatctacg aaatcaatat
    11701 gctccgttgt attttttgtg gtttgtgtga agaagcatgt ccaaaagatg ccatctactt
    11761 aacactttcc aaagaactag taccatcact atacaacaga gaagatttta tatacggaaa
    11821 agataaatta gttatgcctt tggatgttgc attggctaat tctaaaaagc tacattaata
    11881 tgtcaacagt actaattata ttttacatac tatcagcgat aacactatcg actgcttttt
    11941 tgacaatata cacaagaaga ccaattcata gtgctattta tctagtacta tgtttcttct
    12001 caattgctgg acattattta ttgtttaatg ctcaattttt agcaatagtt catgtaatag
    12061 tttactcggg tgctattatg attctgttct tattcaccat tatgttgatg aatttgaaca
    12121 atgaagatga agtgcacaaa ccaagaatta caagacttgg agctattatt atttttgtcc
    12181 ttgttagtat tatcatgatt tctgttttta tcaattcaaa aacaattatg ggtgattatg
    12241 acaacagtgg tgttgattat caatcgataa aagttctggg taaagtattg ttaaacgaat
    12301 atatggtgcc atttgagttt gcttctattt tattattagt agcaatgatg ggaacggttt
    12361 tattatctaa aaaagaaaaa tcaggaaaat aatggggaat attttaaatc aaataggtat
    12421 tgaaaattac attttcctat ccgtattact tttttgtatc ggagtttttg gagtgttgta
    12481 tagaagaaat gccatcatcg tatttatgtc aattgaaatt atgctgaatg ctgtgaactt
    12541 attgtttgtt gctttctcaa cctatcatca agatgccgaa ggacaaatat ttgttttctt
    12601 ttctatggct gttgctgctg ccgaggttgc agttggattg gcaattctag tttctatttt
    12661 tagaaattta ggttcaatcg aaattgataa attgaaaaat ttaaaaggat aatttaatgg
    12721 aaaaaaattt agctttagta ttactcttat ctccatttat tgggtttctt tttaatgttt
    12781 tctttggaaa aaaagtaagc aaaggattgt ccggtgctat cggaacaatt gctgttgtgg
    12841 tttcgttttt agtaagcatt tgttttttta ctcaattaaa tcaaaacggg aaaggatttg
    12901 aaatttcact ttttgattgg atacaagtaa gtaatttcaa attggatttc ggaattcttt
    12961 tagatcaatt atcactttta tggttattgt ttgtaaccgg aattggttca ttaatccatt
    13021 tatattctat cagttatatg cacgacgatg aaaatatgca taaatttttt gcctatttga
    13081 atcttttcat cttcttcatg ataacacttg tagttgggaa taatttattg gtcatgttta
    13141 tcggttggga aggcgttggt ctttgctcct atttattgat tgggttttgg tataaaaatc
    13201 aaccgtataa cgatgctgcc aaaaaagcat tcatcatgaa ccgaattgga gatttaggat
    13261 tcttaatcgg aatgttcctt attgcttatg tgttttcaac tttaaactat actgaaattc
    13321 aaactttagt tataaaaggc gaaaccaatt catcgctact tagtgttgct gctttatgtt
    13381 tattcattgg tgcttgtgga aaatcagcac agttgccact ttacacttgg ttacccgatg
    13441 ccatggctgg accaacgcca gtttcggcat taattcacgc ggctacaatg gttactgctg
    13501 gtatttttat gattactaga atgaatttct tatttgatat cacgccagat gttcaaaata
    13561 taattgctat tgttggtgct attacatcat tggttgctgc atctattggt ttattacaaa
    13621 acgacatcaa aaaagtgttg gcctattcta ccgtttctca attaggattg atgtttttag
    13681 cattaggatt tggagcttat aatgttgctg ttttccatgt aattactcat gcgtttttta
    13741 aagcgtgttt attcttaggc tcaggttccg taattcatgc tttacatggc gaacaagaca
    13801 tgcgaaatat gggtggatta aaaaaataca tgccaatcac tttttggacc atgatgattt
    13861 ctacgttagc catttcggga atatttccat tcgcaggatt ttggtcaaaa gacgaaatat
    13921 taatgaccgc ttttgaacat aatacaacat tgtgggttgt tgcttcagtt gcttcactaa
    13981 tgactgcttt ttatatgttc cgattaatgt atttaacttt cttcaaagaa tttagaggaa
    14041 ccgaagaaca aaaacatcat ttgcacgaaa gtccaagttt aataactatt ccactaataa
    14101 tccttgctgt tttggcagcc atcggtggtg ccattaattt gccaggaagc atgtggttga
    14161 accatttctt ggaacctatt ttagttgcaa aacacgaagc acacgaacta ggtgctcatg
    14221 aatttatgtt aatgggaatt gctttagcag gagctattat cgcaataatt tgggcatatt
    14281 caaaatacat caaacaaagt tttgtgccag aagaagacac tcaaatggtt ggattttata
    14341 aaaccattta taacaaatac tatgtagatg aattttatac ttttttaatt gtaaaaccac
    14401 taaatggctt gtctaatttc tttagaacga ctttagaacc agcattagga caaatagttt
    14461 tcagtttggg aacattagcc aatggcattt caaatcaagg tagaaaaatt caaacaggaa
    14521 gcgttggttt atatcttttt gcattcgttt taggactttg cgccatcatt tcttatttat
    14581 ttatagctcg ataattttta tataatgaat gtaactctat tattactatt acttttaaca
    14641 ggttcgattg ttaccttttt atctggtgat aaattggctt caaaaatagc attgctattc
    14701 agtgtttccg cttttgctgt ttcattatat ctgttaatgc ttttcaacca aggagaaatc
    14761 atcagctata tgagtagttg gatgcaaaaa ccaaatgtgt catttgctct aaaagcagat
    14821 ggtttatcat tagcaatggt tttattaact actggattaa cacctttgat tattctttct
    14881 tcctttggaa actcatatac taatagtaaa aacttttatg cattagtatt gttcatggca
    14941 ttttctatga caggaacttt ccttgcagca gatggattat tgtactatgt tttctgggaa
    15001 ttggctttaa tcccaattta ctttatcgcc ctaatttggg gtaatggcga cgctgaaaaa
    15061 cgtaaaaaag cagtagtcaa atttttcatc tacacattag cgggttcatt atttatgctt
    15121 gtagcctttg cttatttata ttccaaagcg ggaagtttta ttcttgaaga tttataccaa
    15181 ttgcgattat cagaaacaga acaattttgg atattctttg ctttttttgc agcctatgcc
    15241 atcaaaattc caattatccc ttttcacact tggcaagcaa aagtatatca aaaagcacca
    15301 acggttggaa caatgttact ctctggaatt atgctgaaaa tgggattgta tagcgttatt
    15361 cgttggcaat tgcctattgc accagatgcc gcaaaaacct acatgcctta tttaattgga
    15421 gtgagtgtcg ctggtgtaat ttatggttcc gttgtagcat tgcgtcaaaa agacttgaaa
    15481 aaattattgg catattcttc cttagcgcac gttggattaa ttgctgcagg ttgttataca
    15541 ttaactgctg atggactaaa cggtgcggtt tatcaaatga ttgcgcatgg ttttgtaata
    15601 gttggacttt tctttgcagc cgaagtaatt ttcagaagat ttgaaacgag aaaaatcaat
    15661 gaacttggtg gtattcgttc gcaagcttca aagtttactt ctatgtttat gattttggtt
    15721 ttagcatcag ttgcattacc aggaacatac aattttgttg gagagtttac tgttttatat
    15781 agtttgagtc aaatcaatat ttggtttgca gtgtttggcg gaacaaccat aattcttggt
    15841 gcttattata tgttaaaaat gtttcaaaat gtaatgttag gcgacaccaa tactaaagtt
    15901 tttgctgatg ttactctaaa tgaaaccatt gtttttgtta ctatcattgg cttcctattg
    15961 ttctttggat tgtatccaaa accaattaac gaattagtaa atccggcaat tcaaaatatt
    16021 ttgaccgtaa taaatagatc ataaaatgca agtattaata gcaatagcat ccttaggaat
    16081 tttttgttta gtagcagaaa ttttcaatct tagaaaaata ctagttccag tttctgtaat
    16141 tggtttactt gccatccttg gaattactct aaatttttgg tcaatttcag attcgttaga
    16201 cgcacgatac attacgatga ttgcttctac tggtttttca aaagcattca tttcattgtt
    16261 tatcattctc actatcttta tcttagccat gagtaatgat tattacaaag aacacccaac
    16321 aaaattttca gattttatag ccatcaaaat ttttctttta actggagccg ttgctatggt
    16381 ttcttttacg aatttggcta tgttcttttt aggaattgaa gtattgtcta tttcattata
    16441 tattttagcc gctagcaata gattaaacat taaaagtaac gaagctggta tgaaatattt
    16501 cttgatgggt tcgtttgcat caggaattat tctttttgga atttgcttaa tatatggtgc
    16561 aacgggttat tttgatatgg ttgaaataca cgatttgtcg caaggtatag gtttgcctgt
    16621 ttggtttcct attggaatag ctttaatgat aattggtatg ctatttaaaa ttgcagctgt
    16681 tccattccat ttttgggcac ccgatgtata tgaaggttct ccaacgttaa ttacagctac
    16741 gatgagtact ttggtaaaag ttgtggcaat ggctactctt ttcaaacttt taaatggatt
    16801 gcttacatct ataccgccaa aagttgaaat actaatactg ataatttcca ttttgtcgat
    16861 gacagttgga aatattatgg cattacgaca aaaaaatgta aaaagaatgt tagctttttc
    16921 gggaatatca catgctggat ttatgctaat gactctattg acaatggcaa ccgcagctaa
    16981 caatctattg tactatactt ttgcttatgg tttagcagga attgctgctt ttgccgtaat
    17041 aattcatgtt actaaaaaca aagaagacga agctattttt aactttaatg gcttaggaaa
    17101 aacaaaccca ttaatggcag ctgctttgac ggcatcatta ctttccatgg ctggaattcc
    17161 aattttctct ggattttttg ccaagtttat gttgtttaca gatactataa aatcaggatt
    17221 tattgctatt gtaattgtgg ctgtaattaa ttcaattatt agtatcggtt attatttcaa
    17281 acttatttta gcaatgtaca ccaaagaagc aactgctgaa aaagagtcta tttcatctgt
    17341 ttatacgatt gttgctgtac tagcaattat actaaatatt atcattggta ttaatccttc
    17401 tttggtatta aacttgatgt aattttacaa tcatcacact acaaaaaaag tcattgagaa
    17461 gttcttaatg actttttttt gtttctaaaa tgaaatctaa aaacctagaa tgattttagc
    17521 aattgaaaaa taaattaaaa ttccacaaat atcattactt gtggtaataa acggaccagt
    17581 tgctaatgct gggtcaattc caaatttatt caatatcaaa ggtataaatg tgccaattaa
    17641 tgaagcaata ataataaccg aaattaaagc aatagttaca atcatcccaa tttttggatc
    17701 aactccaaga ataaaatgac taccaataaa tagaatggtt gctaaaataa cgccatttaa
    17761 taacgctaaa gaaatttcct ttaatagtcg attcacaata gaaccactta tagattgatt
    17821 tgccaaacct tgaacaataa tggccgaaga ttgaacacca acatttccag ccattgcagc
    17881 aattagtggt gtaaagaaaa ataattcgcc atgtttttcc attgctccgc caaataagcc
    17941 aagcatttct acagatataa atccaccgca aagtgctaaa accaaccaag gcaatcgcgc
    18001 ttttgttaac tcaatgatgc tgtcatctgc ttcaacatct tgcgaaatac cagcagccaa
    18061 ctggtaatcc ttatcggctt cttctttgat tacgtcaaca atatcatcaa tagtaattct
    18121 acctactaat cttcccattt catctacaac tggaatggct tccaaatcgt atttctgcat
    18181 aattcgagcc acatcaacac ttggcatgtt tacttttact gaatcaactt ttgggatgta
    18241 aacttcgcta ataggtgttt tggtagatgt agttaataaa tcttttaaag aaagtcttcc
    18301 ttttagccga ttttcatcat caacaacata aatggaatga acacgtgtaa cgttttcggc
    18361 ttgaatgcgc atttctttta cacaagtcaa tacgttccag ttttcattaa cttttaccaa
    18421 ctcttttccc atcaaaccac cagcagaatc ttcgtcataa cgcaaaagat caacaatgtc
    18481 tttggcgtgt tctaaatctt ctaattccga aattacctgc tcttttttgc tttctggtaa
    18541 ttcattgatg atgtctgcag catcatcggt gtccaattcg tcaagttctt ctgcaatttc
    18601 tttggcagaa aggccacgta gaattttttc acgaacatct tcttcaagtt caagaagaat
    18661 ttctgctgtt ttctcactat caagaatacg gaaaacataa gtagcatcat caatacttat
    18721 ttcatcaaaa atctcagcaa tatcagcatg gtgcatatca ttaagcaaca attctaattc
    18781 ttggtcgttt ttgactagaa tgagttgctc aatttgttct attaattctt tgctgatttt
    18841 aaactgcatt ggcggctatt ttttgtgtga tttcaacaaa ctgttctaca gataactgtt
    18901 ctggacgaag gtcaaagata gtatcttctt ttaaattatc tgataattga aatgatttta
    18961 aactatttct aagcgttttt ctacgttgat taaaacctgt ttttactaca ttaaaaaata
    19021 atttttcatc acaaggtaac gagaaatctt cttttcttct catgcgcatt acacctgatt
    19081 tcacctttgg tggcggattg aaaacttctg gctccactgt aaaaagatac tctacatcat
    19141 aaaatgcttg cgacaaaacg gaaagaattc catacacttt atttcctttt ttagagcaaa
    19201 ttcgttcggc tacttctttt tgaaacattc cagaaaactc tggaacttga tttcgcattt
    19261 ctaaacattt gaaaacaatt tgtgtagaaa tattataagg aaaattgccg atgatagcaa
    19321 aaggtttccc ttccaaaact tcgttgatgt tgtattttaa aaagtcttta gaaattattt
    19381 tgttatgcag ttttggataa tgtgaatcca aataggatac tgattcggtg tcgatttcaa
    19441 tcacgtaagt atcaatttct ttttccaaaa tatatttggt caaaacaccc attcctggac
    19501 caatttccaa aacggtttca tagcccgaaa aggacaaagt atcggctatg tttttcgcta
    19561 tgctttcgtc ggttaaaaaa tgttgcccaa ggtgtttttt ggcttttact tgcatgttat
    19621 gtgatttgtt ttttcaagaa aatggaaatt aatattccga aatcaattct aattcagttc
    19681 tgaatgccaa cattttttca ccgaataatt gcattccttc ttctcgaagt tttggtgcat
    19741 cttcgtcgta atatttttgt agcgtttctt tgcaatcagt tgtaaattgt actgcatagg
    19801 tcattccgcc catttcttca tctactaaaa cccgaaccat tcgtgctgaa gtaaattttc
    19861 cagtatttaa tacatcgtga atgtgtttgt gatgcatcca aatcatccat tggtcgtgaa
    19921 cgctttcgtg tatattgatg gtaacattat atataatcat gtaattttag atattaaaat
    19981 ttagatatta tttcctattt agcacttcga ctgcgctcag tgtgacaaaa ctgcctactg
    20041 caactgctca ctgaacactc atttctgtac ttcgattgtg ctcagtgtaa caaaactgac
    20101 aactacaaag tagtatcgcc acgcaattgc ctgtatttct tcctgctatc aacgtagtaa
    20161 atactgtctt catgcttgaa aatcatttct tcataaagtg gtttggcttt ttcagcatca
    20221 ttgagtttta aattatatat ctcagccgaa tagtataaag cttcatcaat atatattcca
    20281 tctttatgat tagtgattat ttcattgtaa ttttctaagg ctttagtata atcgccttgt
    20341 ttttcataga tttttccaat tcggagtaat gtcacggctt caatttcttc ccctttatgt
    20401 tcttttaaaa tcaattgaaa ttgggataat gcgtcttgtt ttttattttg atacaacaga
    20461 aaatcaccac gagaaaattg cttcaaagct acttgtgtag aatcttctac ggtgttgtcg
    20521 ctaatcaata aaaacaaatc caacgcatca ttggcaatta actgagtgga ggccgatttt
    20581 aaaactttta gttgatgcga tgcccattta aaatcagctt tgtaataact ggtttttgcc
    20641 gtttttaaac tcgcttcttg accaataaca tcgccgctta aatcattttc tatttgagaa
    20701 taataaatca aggcttggtt gaatttttct tcaaacaata aaatatctgc caaatccatt
    20761 tttacttctg ccaattggta tttattcaac ggcatgtcca tggcattttt taaaatcgtt
    20821 ttggcttttt cggtgtcttt taaatgaaag gctgaaaaat tagcatccaa ttttaataat
    20881 gataaagtat atggactaac gccaaattgt ttaatcaaca catccaattc ttgggtaatt
    20941 gctggatagt ctttttcggt tgcaatatca attttcattt caaccaaata agaatgtgct
    21001 tgaattaaca aatctaaatt attggtattg tctaaaacaa aaatcaagat ttctttagca
    21061 gcttccgaat ctttatcgtc tatagccatt tcggctaaat tcacaatatt agaaaacgat
    21121 tccggatttc gtttataaat cgctttttgc tgaataaaag ctttgctata ttcttttaat
    21181 tgcacataat accaacttaa aaaatcgttc caaaataaat cttgtgattt ttgggttcga
    21241 agaagtagtg cttttcgaag tgtattattg aaattctcat ctgtgtcttc cgtcataaat
    21301 cgggatagct gattttgaat aattggaagg ttttgagggt tttgaaaacc ttcggcaaga
    21361 aaggtttcaa tcatcaaatc ggtattccct ttttgtccat aaagtactgc catttgaaaa
    21421 ttaaaactca tttttggttc tttttccaat gccgtttgat aggctagcaa tgcataatct
    21481 atcaaagatt ttcgttcaaa aacataagca attccgtaaa cttcgttagc attgattttt
    21541 attttttcaa tagcttggtc ataatattta gtagcttttt cattattctt ttgcaattga
    21601 tagttataac ccaattcaac taaaagattg ctttgacggt atttgtccaa tcgttcttga
    21661 agtgcttttt ccgccttgtc atattgttct agttgctgat aacattctac aacacgttga
    21721 aaataattgg aatttccaac ttgcattttt agtaattctt cgtaggacac caaagctttt
    21781 tcaaattcgc cacggtcaaa ataattgttc gccaactgtt cattttgtgc ttttgcacaa
    21841 atggttagaa aaagaaacag aacaataaaa aattttttca tgtaatggag ataaaaaaca
    21901 atgcactaaa gtaacgtttt ttgtgtcaat ttagtgcatt gtttaaggaa gattaacgtt
    21961 ttagttaata atatcgaaac cagtatatgg tcgtaaaacc tcaggaatta caattccttc
    22021 tggtgtttga taattttcta atattccagc aagaactctt ggcaacgcta atgaacttcc
    22081 atttaatgta tgtgccaatt gggtttttcc gtcttttcct ttgaaacgca atttcaatcg
    22141 attggcttga aaagtttcaa aattagaaac tgaactaatt tctaaccaac gatcttgcgc
    22201 tgtagaaaat acttcaaaat cataagtcaa tgccgaagca aaactcatat caccaccaca
    22261 aagtcttaaa attcggaaag gtaatttcaa ttcgcgcata atgtttttta cgtgttcaac
    22321 catgccgtcc agagcttcat acgatttttc aggatgttca atacgaacga tttctacttt
    22381 atcaaattga tgcaagcgat tcaaaccacg aacatgcgca ccataagaac ctgcttcacg
    22441 acgaaaacat ggtgtataac ctgtatataa aacgggtaat tcgttttctt gcaaaatcac
    22501 atcgcgaaat aaattggtta ctggaacttc agccgttgga attaagtata aatcatcaat
    22561 accaacatgg tacatttgac cttctttgtc tggcaattga cccgttccat atcccgaagc
    22621 ttcatttacc aaatggggca cttgtacttc ttgataacct gcttctgtgt ttttatctaa
    22681 aaaataggct atcaaagcac gttgcaattt ggcacctttt cctttataaa caggaaatcc
    22741 tgcgccagtg atttttacac ctaattcaaa atcaataata tcatattttt ttactaaatc
    22801 ccaatgtggc attgcgtttt catgcaaaac aggaatgtag ccttcttcaa aaacgttcaa
    22861 attttcttca ggagttttac caacaggaac aatttctgcc ggaagatttg gcaacaaata
    22921 cattctttgt aacaattcag cagcaaaacc atctactttt tcagaaaggt ccttgctggt
    22981 ttctttcaat tgtgatgttt ttaactttaa tatttctgct tttgcttttt cgccgttttt
    23041 catcatttcg cctatggcag atgataattt gttggcttca gctaaagtat tgtctaaatc
    23101 attttgcgta cttcttctgt tttcgtctag ttcaattact tcttctataa tggtttttgc
    23161 atccaaatgc ttttttgcca aagcagtaat aactttgtct ttgttttctc tgatgtaact
    23221 tatttgtaac atagttttct ttaataaggt tgcaaattta atgaaatgtt ttagaaatag
    23281 attcagttcc ttaaaaactt gtttttttct aatttgatta aaatcaactt tatagtcttg
    23341 ttattaatct ttttttaaga caaccttact tcaatatgta ttatattcgc aaaaattact
    23401 atactatcat gaaaaatatc tatctattct ttttctgtat tagcgtttct tttgtcttta
    23461 gccaaaaaaa agaaagcact accaaaacaa tagcggaagc agaaatgaat tcagcatcaa
    23521 gaattgctaa ttttcaagca aatccaaata cagctaatta cgatgtaacg tatcaaaaac
    23581 ttgaatttac tgtaaatcca gcagtaaaat ttgtgagtgg caaaataaca accacgttta
    23641 cggcattgac aaacatgaat tcaataacat ttgattttgc taatgaatta actgcaagcg
    23701 ttataaaaaa aggtgcaaca aatttaagtt ttaccgaaaa ctcgaacaat gaattagtga
    23761 ttacacttcc atctacacag ttagcaggta cttcggctac catcgaagta acttattcgg
    23821 gtgttccacc aaataatgga tttgactctt ttgttaaaag tacacataat ggaactccaa
    23881 ttatttggac actttcagag cctttcggtg ctagagattg gtggccttgt aaacaagatt
    23941 taaatgataa agtagatagt attgatgttt atataacagc tccttctcaa tacattggtg
    24001 tttctaatgg tattgagccc gaagctccag taattaatgg agctaacaaa acaacccatt
    24061 ttcatcatgg ctatccaata cctgcttatt taattgcaat cgccgttaca aattacactg
    24121 tttataatca agttggagga acagctccaa atgaatatcc aattataaat tacatctatc
    24181 ccgaaaattt tgcatcaatt caaccacaat tgagtgaaac acctgccatt ttagagttgt
    24241 atgagaattt atttgaaatt tacccattcc acaatgaaaa atatggtcat gctcaatttg
    24301 gttggggtgg aggaatggaa cacactacag tttcatttat ggtgggtttt gatagacaac
    24361 taattgctca cgaaatgggt catcaatggt ttggcgacaa aattacttgc ggttcttgga
    24421 aagatatttg gctcaatgaa ggttttgcaa cttatttggc agctttagtc atcgagaatt
    24481 ttgatggttt agattcattc attgccgaaa aaatagacat ggtcgattat ataacctcga
    24541 gtcctaacgg gaatgtttat cttgatgatg ttgaggcgac taacgtaaat agaattttca
    24601 gcagtcgatt aagctataac aaaggcgcca tgattttaga gatgttacgt ttcaaaatgg
    24661 gcgatactgc tttttttcaa gctttacgta attatttagc cgacccaaat ttagcttata
    24721 aatatgccgt tacaactgat ttaaaatcac atttagaagc agtttacggt caagacttaa
    24781 ccgagttttt tgatgactgg gtttacaatg aaggataccc aacttacgac atcactgctc
    24841 aaaattgggg ttctgggcaa gcaaaatttg ttgtttcaca aacgcaatcc gatgcttctg
    24901 taagttattt tgaaatgcca gtgcctgtaa gagtttatgg ctcaaatggt gaagaacaag
    24961 atattgtttt agaaaacact tttgacaatc aagagtttat tgttaatgtc ccttttacta
    25021 tcacaaatgt tgtttttgac ccagaacagc atttaattgc aagaaattca acagcaacat
    25081 tacaaaactc taatgttgaa tggaatcaga caatttctgt ttttcctaac ccaacaaatt
    25141 ccattttaca tattcaaaaa ccgaatgatt tatcaattga atctgtaaca ctttataatt
    25201 ctttaggaca aatggttttg aaatcaaatg aaaatgattt gacaatcgaa agtctttcaa
    25261 atggagttta tgaacttcaa ttgaatacta atcaaggtaa atttcacaaa agaatcataa
    25321 aacaataatc tatttttgaa aaatattcca aaggcttgtt cgaaaacctt acttttgtgc
    25381 gcttatttaa aaaagaatta aaattaaaga atggaaatcg taattaaatt atctcaattt
    25441 ttattgagtt tatcaatact tatcatactt cacgaattag gacattttat tcctgccaaa
    25501 ttatttaaaa ctcgagttga aaagttctat ttattttttg atgtgaaata ttcattatta
    25561 aaaaagaaaa taggcgaaac cgaatacgga attggatggc taccacttgg cggttatgtg
    25621 aaaattgctg gaatgattga cgaaagcatg gacaaagaac aaatgcaagg cgaagcacaa
    25681 ccgtgggaat ttcgctccaa accagcttgg caacgtttga ttattatgct tggtggggta
    25741 accgttaact ttatactagc attcattatt tatattggaa tgacgtatgt ctatggcgat
    25801 tattacatca aaaacaacga ccttaaagat ggagtttggg taaccaatcc agtagttgaa
    25861 aaagcaggaa ttaaaactgg cgatagaata gtgtctgttg atggaactcc tgttgagcgt
    25921 tttgtacctt cggttagctt ggatatattg atgtctaaaa aaataattgt tgaacgcaat
    25981 ggtcaaaatg tagaaataga tttaccagtt aatttcattg gtaaaattct tgacgaaaat
    26041 aaaaaagaag tcatcagatt aagaattcca tttgttgttt caagtatttc tgacgaatca
    26101 gccaacaaag atgttttaat ggctaaggat atattagtat cagtaggtgg aacatctgca
    26161 aaatattatg atgaagcatt aaaatatttc gaagaacata aaggtcaaaa agtagcagct
    26221 gttgttctta gagataataa agaggttgct gttagtttgc aaatcaacaa tgaaggaaaa
    26281 ctaggtgtta tatctggtgg acttaaagaa gataatttag aaaaattagg ctactataaa
    26341 tttagtaaag aaagctatgg tttcttagaa tcatttccaa taggaattga taaaggagtt
    26401 acacaattaa gtaactattg gaaacaatta aaagcaatat tcaacccaaa tacgggagca
    26461 tacaagggtg ttggcggctt tgcggctatt tataatgtgt ttcccgacac ttggagctgg
    26521 gaaacttttt ggaatattac cgcattacta tcaattatgt taggagtaat gaatttatta
    26581 ccaattcctg ctttagatgg tggtcatgtt atgtttttat tatacgaaat gataacagga
    26641 aaaacaccaa gcgataagtt tatggaacgt gctcaattag ttggttttgt tttgcttatt
    26701 tcattgttgt tgttcgcaaa tggtaatgat atttacaagg caattgtcgg caaataattt
    26761 tttttaaaaa tagtagttaa attatttgca aaaaataaaa ttcgttctat ctttgcaacc
    26821 gcttaaaaga taaaacatct tcctccttag ctcagttggt tagagcatct gactgttaat
    26881 cagagggtcc ttggttcgag cccaagaggg ggagcacaaa acaaaagact tcacagagat
    26941 gtgaggtctt ttttgtttct tgaaaagaaa tacatcctaa acgaacttaa ataaaacaaa
    27001 aaaaccttca agtttccttg aaggttagta cccggagtgg gaatcgaacc cacactccta
    27061 ggaacacgag tttgagtcgt gcgcgtctac caattccgcc atccgggcaa agtgttaaat
    27121 gaaatcatta aatttaattt ttagaaaaat taattaaaac aataacatcc aagcaaagga
    27181 tatgctttag aggttgcaaa tgtaaatatt ttttcttcac acaaacaaaa aaatagttaa
    27241 aaatatgctt tcatagttga attttattcc taaatttgca cctcggtaaa acgaagcaca
    27301 actaactaac tacacccgag acgaatacac acgaaaagaa ttaggcaaca tcttaatcat
    27361 ttagtagtaa gcgtagcggt ataaaacaac aaataaaaaa tgtcacatca agaacctgaa
    27421 gcaaaaatat ttgcttgttc ccaaagtgtc cacctcgctg aagaaatagc caaaaactat
    27481 ggaattccgc ttggtaaagt tacgttctca aaatttagtg acggtgaatt tcaaccttcg
    27541 tttgaagagt ctattagagg tttacgagtt tttattgttt gttctacttt tcctagttca
    27601 gacaatttaa tggagttgtt gttaatgatt gatgctgcaa agagagcttc agccagacac
    27661 attactccag taattcctta ttttggctat gcgagacaag acagaaaaga caaaccaaga
    27721 gttccgattg gagcaaaact agtagctaaa ttattagaaa ctgctggtgc tactcgaatt
    27781 atgactatgg atttacacgc tgaccaaatt caaggttttt ttgaaagacc agtagatcat
    27841 ctgttcgctt caactatctt tttgccttat gttaaaagtc ttcaattaga taatttgaca
    27901 attgcatcac cagatatggg tggctcaaaa agagcttatg cctactctaa atttttagat
    27961 tctgatgtgg ttatttgtta caaacaaaga aaagctgcaa atgtaattgg aactatggaa
    28021 ttaattggag aagttaaagg aagaaatgta gtattggtgg atgacatggt tgatactggc
    28081 ggcacattag ccaaagcagc tgatttgatg attgaaaaag gtgctttaag cgttagagct
    28141 atttgtactc atgctatttt atcaggtgat gcatatgaaa aattagaaaa ttctcaatta
    28201 agcgaattga ttgtaacgga ttcaattcca ttaaaaagag aatcaaataa aataaaagta
    28261 gtgagttgtg caccactttt tgctgaagtt atgcacatgg tgcaacacaa caattccatt
    28321 agtggaaaat ttttaatgta aaagacaatt ataagttgtc aatgcatttt tgaaatagaa
    28381 ttattattaa ctatatatta tttacaatga aatcgattac gattaaagga tcagaaagag
    28441 aaagcgtagg caaaaaagca acaaaagcct tacgtgatgc tggaatggtt ccttgcgtta
    28501 tttacggagg aaatcaacca gtacattttg cagcagaaga aaaagcattc aaaagcttgg
    28561 tttacactcc aaacgcacac acagttgtag ttgatttggc aggtaaaaca tttaatgcaa
    28621 ttcttcaaga cattcagttt cacccagtgt cagacagaat taaccacatt gatttctttc
    28681 aattaaatga cgaaaaagaa atcatgatgg aagttcctgt aaaaataaca ggaacctctc
    28741 cgggagtttt acttggtggt gaattacgtt tgaaccaaag aagattgaaa gtaaaagctt
    28801 tgccaaaaaa ccttcctgat tacgttgaag ccaacatttc agaattacaa atgggtaaca
    28861 aattatatgt tactaaatta gttcttggag attttaaatt aatgcatcca gacaacactg
    28921 ttgtagcgca agtgaagatt tctcgtgctg ctatgaaagc cgctcaagaa gcagcaaaag
    28981 cagaaaaaac tgcagcaaaa ggaaagaaaa aataattttc aatatacctt ttcaaaaaag
    29041 catcagttat ttactggtgc ttttttattg gtttcaattc tattactttt acaccatgaa
    29101 atggttattt tcacttttta aaaaacaaat caaggaaaat caaatcaaca tgaagaaatt
    29161 tttaattgtt ggattaggaa atattggttc cgaatataca aacacgcgcc acaacattgg
    29221 gtttaaaata cttgattatt tcgcttcaaa agaaaattgc tcttttcaaa ccatgaaatt
    29281 gggtgatata gcagaatatt ccattaaagg aagaaaacta atttttctga aacccaacac
    29341 ctatatgaat cttagtggta aagcggtaaa atattggatg gaaaaagaaa acattgaaaa
    29401 agaaaatatt ctagttataa cagacgattt gaatttatct tttggaagca ttcgcattaa
    29461 agctaaagga agtgatggtg gtcacaatgg tttaaaaaac attcagcttt tgttaaacac
    29521 aaccgaatat ccacgattcc gttttggtat aagtgaccag tttaaaaaag gacaacaagt
    29581 agattatgtt cttggcgaat ggaatgaaga tgaaaacagt aaactaactg aacgtttaga
    29641 tttatctatt aaaatcattc aatcatttgc cctagctgga ttaaacaata cgatgaatga
    29701 gtttaatgga aaataaaaaa gcggtcaaaa ttttgaccgc tttttaaaat tatttaaaat
    29761 tattttttaa tcaacttatc tgatttcaat aatctatttt cattataaat tcttaaataa
    29821 taaactcccg tttctaaatt gtcaacataa attttattat taacttcatc taatttttga
    29881 gatttaacca tttgaccgtt tgtagagaat atttcaacat aatctacatt gatttgaggt
    29941 gatgtaatca taaaaaattg agatactgga ttaggatata ttgcaataga ttgaatagct
    30001 tcttgttccg ttacgttcaa ggattggcac ccatttgaag tcttcaaact agctcttgga
    30061 ccattcatag ttgcttgagc aattgccttt tgaccagctg taaaaaatgc catacaagca
    30121 tctggcacat aatccatata attcataaac atcgctccgt tggttgaagg agaacacata
    30181 taagtgttat taggaaaaga tacacaatca taataagcat cgtatgttgc tggggtgtct
    30241 gctactccat catctgcatc gcaattacca tcaccccatg gatgatctaa tccaaagtaa
    30301 tgaccaattt catgtgtagc tgttcttcct ttattatatg gtgcttgagc tgttcctgta
    30361 tttccaaaaa atttatctcc aatacacaaa ccatctctat ttgcataaga agtaccaaca
    30421 atagaaggta aataagcaaa acctaataat gtattgtctg taaatcttcc cacccatata
    30481 ttaagatatt ttgttggatc ccaaactggt aatcctgcag acgtatagta ttgattttca
    30541 aaaacaaatg aagaggctac tgattttctt tcaattccat ttgtagcagt accatctggt
    30601 ttttgtgttg ccatacagaa agcaagttca aaatcagctc ctaatggtct aaaggctgtt
    30661 ggaacaactg agttaaaatc agtatttgtt tttctataat cagcatttaa tattcctatt
    30721 tgtgataaaa tctgtgcttc actaacattt tgcgcagcgt ttttatataa tacatgcaca
    30781 actactggaa ttgtataaac cacactggta gatctattta atgcagcatt tgtattattt
    30841 ggatttcgaa taaaatccat agtttcttca tgatgaatct taaactctgg attattttga
    30901 atcaattcat tcatcttgtc attagtatga catgttcttt gtgctatagc gactatgctg
    30961 ttaacgaaca agaaaacaat aataattttt ttcatggttg tatttagtgt aaattttaaa
    31021 aaaagttctc aaatttactg aactttagtt ttttagcaaa tataaatgct taaatttaaa
    31081 aaataagaca aaaaaacagt ttttaacaag aatagtcgag tttatttttg tttaaagagg
    31141 aaaggtaacg tatctatttt tttaaatttt tacttcattt taaaaaaatg gtaagctttt
    31201 tgactatctt tatttctaaa ttcataatta tgaaaaaaat ttttctaatt ccattggcat
    31261 ttagtttgtc attttgtaat tgtaaaaaat cagtagttga aaattcaaaa actacttcta
    31321 tagaatcggt ttgtcctgat aatggtattt gtaccgttaa gatatataag aacaaaagtt
    31381 tagaagtaaa aacagatgat ttaggcggta tttattatca aatgctagat aatacagcca
    31441 catctgtaat tcaatatcaa tacaataaaa cagttgaaga aggattacaa gatggtcaac
    31501 atcgtgaaga aatattattt gaaattaact cttctaaacc tttactaaat ttgaaagata
    31561 aaaatcttca agaagtaaaa atgatatttg gaagacattg tttttgtaaa ggtcaagccg
    31621 gctattttaa agtagaggaa ggttctttat cccttgaaaa caaaaatgaa actacaactt
    31681 ttgaattaga ttttaaaatc cataaagtgc ctcaattatt tgaaaaagtt aaagctactg
    31741 ttcaataaaa aaggagatgt ttaaaaaaca tctccttttt tagtattagt aacgttaaga
    31801 ttattctacc gctatttttt tcacttgttt actaacacca tcagaaactg taactaaata
    31861 aatgcctttt tgaacatttt ttaattgaat attttcgtta aataatggtt tgttttcaaa
    31921 aacttgtttc attatttctc tacctctaat atcatgaacc atcacattta catcgttggt
    31981 tgagttactt gaaaactgaa tggtaaagtt accattatta gggtttgggt acaaagcaaa
    32041 atcattaaat ccgatattgt tttctggagt acttaaggta acagtttgtg tacatacttc
    32101 aacggaccag ctatttactg ttcctgaatc accagcaaaa ttatcataaa ctctaaatgt
    32161 ccaagttcct tgtggatttt gaccattaaa tgttgctaaa cttccagaag gtgaaactgt
    32221 ttgtaatgtg tttccaccgc tacagttaat acctggagag ccatcactaa acttcatgtt
    32281 caaagtagca cttgcgtttc cacagcctct atcaaataat tttacaaaag tagttgggtt
    32341 tgctggacta ctaatttctg taactacatc ggataaataa gtgtgagtaa tactatttga
    32401 aacattaaca tctgatatag ttcctgttgc tgggataact aaagttctga tggtgtaatt
    32461 tcctggtgcc tgatcaacaa ttgatattgg acctcctgta aaattataag tattacattc
    32521 gtttacaatt gtatagccta gtgcaatgtt ctttgaaact gtataaaaaa tattgccaac
    32581 tgcttcaatc ataattcgac aagtagtaga tgctacaaac ggtaacgtta ctgattccga
    32641 gccgtcattt aaagtattgg cagctaaaac tgttggaaaa gtaacgcctc catcagttga
    32701 aagtaaaata tttacatttg aggtattaat accatttgca gttgttccag ctacatccca
    32761 agtgatggtt tgtgtagaac cttgattcca tgatacattc tcaacatttg ggcttgtgat
    32821 tttgaaaggg ccaacatttg caaatactac attcatgtta tctctatttg tttgaccacc
    32881 atttggcatt ctattatctc taacggttag cgcaaaactc attgttcttg caacgcttgg
    32941 aactacttcc caagttggtg ttaaatttcc attcaagaca tcagcaaaag aaggcatata
    33001 tcgatttggt gatgttgaag gcggaagaga tctaaagtta ggtccagttg atgatgttgc
    33061 taatggtggt tgtgttgaaa cttgagtatt cgtttgttcc caacaatatg ttagtgcatc
    33121 accattagcg tctgttgcac ttcctttcaa gataaatgca gttccatttg gaataacata
    33181 attaagtcca gcatctacaa ctggcgctgt gttaccgtta agtgtattgc ctgaacatgt
    33241 tccattattt acaacaaaag aggtcatttg agcgatacta acggcatgaa aatgtgcgtc
    33301 agaatttcct tgaatatctg gagcacaaat accagcataa cccattattg ttgttccgct
    33361 tcctggttct acagatgttg ttgtactaat gtttccacca caactgttat taaatgtatg
    33421 tgtggcacca aattgatgac ccatttcatg ggctacataa tctatatcat agggatctcc
    33481 aacaggagaa tcagaacctg taattcctct agcttttgat gaagaacaag gaactccaag
    33541 cccagctaat ccaccaccac ctgtactaac ggtatgtcca atatcataat tggcagtacc
    33601 aattgctcca tctataacgg tttgactttc atttattaat gcgtttgcat tgttattcgt
    33661 aaaattatct gaagtaatga atatgatgat atcattatta gctactaaat tcattcgtaa
    33721 agacatgtct ctttcataaa gaccattaac tctcgtcatg gttactacca tagcagacaa
    33781 aactgcagct tttttttgag ccaaagtacc cgaatttaat cctgctgctg ctacatgaaa
    33841 agctgcatac tcaattgtac aagccatcgc taaacggtat tgtctaaaag taccgtcatt
    33901 aaccaatctt tcttgtaaat tattggtatt tgcatcaaca aaagcagttt catcttgtac
    33961 taaacagttg aatttatttt cttgtttgtg aatactcttt ttgttataca caatataata
    34021 gttcaaatct ttagtataag tatcaatata gaaagttcca gattttccag aaagtgacat
    34081 tacatgaagc ccaaaaagtg tagtgctaaa gcgaattgaa gcagaagcat catctacacc
    34141 tttgcctgaa tacgatttga tttcaggaaa tttatctgac aatccttttt ccataacagg
    34201 tgcttcataa attcgataat tttgaatttg accatttgag tttgggaaag ctattacaac
    34261 atttgattta gtagtacttg aatctaatgg tgcattttgt aattgattct taagagcttc
    34321 aaaatttaac gcatacaaat catattcatt aggtattgta gcgcgatcca ttttgggcaa
    34381 agcactcact ctttcatttg aggccttaac ccaaagtgaa ctttgaccat aactgcttgc
    34441 gaaaaatgca aaagcaatta caaataatag tagatttttt ttcattattg aaataaatta
    34501 attagagtta taagtgtaaa gataaccaac tttatactat taacaaagtg taatttattt
    34561 tttcacaaaa aagactttta ataaacatta gtcaaaacat cattttaatc cttttttttt
    34621 agtcccaatt aattattatt tttagattct aaaacttact tttgcaacaa tttataatat
    34681 actttcttga aactatttca ttctatttca gattttaagt gcaaatcaaa aacgattgta
    34741 actattggta cttttgatgg tgtacatctt ggtcatcaga agattttaga aaaaataacc
    34801 tctaatgcct ctctgtcaaa tctcgaaagt cttgttttga ctttttttcc tcatccaaga
    34861 actgttttgc aagaaggaac agaaatgaaa cagttgaata ctattgaaga aaaaatcaat
    34921 ttactagata aaattggcat tgataattta gttattcatc cttttgacaa agaattctca
    34981 agattaacag ccgaagattt tgtaaaaatc gttttggttg acaaatttca tgcttcaaaa
    35041 attattattg gatttgacca cagatttgga agaaacagaa ccgcagatat taatgatttg
    35101 atagattttg gcgaaaaata tcaatttgat gtggaacaaa tttctgtgga agaaataaat
    35161 gatgtttcag taagttcaac aaaaattaga aatgctctaa acgaaggcga tgttacacta
    35221 gccaatactt ttttggatta tcgttattcc ataaaaggat tggttataaa aggaaaacaa
    35281 ttagggagaa ccattgggtt tccaacagct aacattcaaa ttaacgaagt gtataaatta
    35341 attccaaaag aaggtgttta tattgtttgt tgtgacgttg aatcgtccac atattatgga
    35401 atgatgaata ttggactaaa cccaactacg gacgctaaca accaaaaaca aaaaattgaa
    35461 gtgcaccttt ttgattttaa tgaagacatt tatgaaaaac caattaccgt ttctttctta
    35521 aaaagaatta gagatgaaca aaaatttgaa tcagttgctg ctttaaaaaa tcaattgtca
    35581 cttgataaac tcaatgctat ttcatacata gaaaaaactt tatcctaata ataacctttt
    35641 ttctatttca aaaaatctaa tttttttgtt aaaaaaataa atttttacaa tatttatctt
    35701 aactttatgg ttatcaaatt attaatttaa tagttagtac catgaaatta tctaaagaac
    35761 ttgttaatgg atttttaatc tttgctggaa tagctatctt ttttttagct atggaaatag
    35821 ccggattagc aaataacttt tatttaagat tttttaatgt cctatttgta ttttatggtg
    35881 tgaatcgcac tgtaaaacaa aatattgccg aagggaaaaa tgggtatttg aaaaatatgt
    35941 tttctgctgg attaactgct ttcattggtg tgattttaag catcatcagc ttagttgctt
    36001 ttattcatta caaaggcgga gatgcttata ttgctaatct ttccgaaaaa tttctttttg
    36061 gcggtgctag tgtaaacgaa tacagtattg gattactgtt tgaaggatta gcatcaggca
    36121 tgattatcgt ttttatcaca ttgcaagttt ggagaggaaa aactgtattc aagtaaaatc
    36181 aatgataatc ataggtcaac ttattttgtt tctttataac ttatccctat ttttgatgcc
    36241 caataaacat cctatatgag tatcacaaaa cacaactgga caaaagaaga aattattgct
    36301 atctacaaca agcctatgat ggatttgctt tatgaagcag cctctattca tagagaacac
    36361 catgatccaa acgtggttca agtttctacg cttttatcta ttaaaactgg cggttgtcca
    36421 gaagattgtg gctattgtcc acaggcagct cgataccata ctaaagtgga aggaaatgat
    36481 ttaatgtctg tttcgcaagt taaagcacaa gcattaagag ctaaagaaag tggctcgtca
    36541 agagtttgta tgggtgctgc atggcgcaat gtaaaagatg gtgaagaatt tgaccaagtg
    36601 ctagaaatgg taagaaccat caacaaacta gacatggaag tgtgttgtac tttgggaatg
    36661 attaccgaaa accaagcaca acgtttagca gaagccggac tttatgccta caatcacaat
    36721 ttagacactt cggaagatta ttataaagaa gttatttcta ctcgtgcttt tgacgataga
    36781 attcaaacca tagaaaatgt tcgtaaaacc aacgttactg tttgtagcgg tggtattatc
    36841 ggaatgggtg aaagcattga agatagagca ggaatgttag tagctttatc aacattaaat
    36901 ccacaaccag aatcagtgcc aattaatgct ttagtagcag tagaaggtac tccgatggaa
    36961 aacgaaaaac ctgtagaaat ttgggaaatg attcgaatgg tagccactac ccgaataatc
    37021 atgccagaaa ctcaagtaag actatctgct ggaagaacag aaatgtctcg cgaaggtcaa
    37081 gccatgtgtt tctttgctgg tgccaattct atttttgctg gagacaagtt attaaccact
    37141 ccaaatcctg atgtgaatga agacatgaaa atgtttgaaa ctcttggatt aataccccaa
    37201 aaaccattcg ttaaagtaat gcaaccaaca actgttgaag ctgaagattc tcagtttaca
    37261 ccattaggcg aaaaaccaag atggacaaga ccaggacata ccattgagcg caatcttgaa
    37321 gcttctggaa aagggaaata attgtattgt acatttatta caaacaaaaa agcatcaatc
    37381 tcttgatgct ttttgtgttt agtttataat gattttcttc gagaccaaaa ctccattggt
    37441 aagcgtaatt tttataatca atgcttggtt tctcacattg tttgaaatgg aatacataag
    37501 agtatctaaa ttactcatcg tataaacttc tcttcctaat aggtcaaata tctgaacttg
    37561 acttatcgtt tctgtattag aaataatgtt tatttgagaa tgatttgtag aaatcactac
    37621 ttgattggtg ctatcaaaat tggcatttcc taatgcttgt gtggtatagc gcaatatgaa
    37681 tctatcttca aacatgcctt ttgttgtagt aaaggtgtaa ggtgcatctc ttaaatcatg
    37741 aataataccc agtagcatat cttctatata aatagcttga ttattagcaa acaatccatc
    37801 aactgctgca atggctattt tataattacc aacagaattg gaattatatc ctaacctaat
    37861 agtatcttga ttatcaaaag gcaatgtttt tccttgaata cacaatggtt gtaatggaac
    37921 atcatttgaa aaagaataca atttcaaccc agttgctttg gttactgcat catatagtct
    37981 gtctgtattg ttagaagcgt ttgtaacata tccaactaga gttctatcag tttgattatt
    38041 aggtgcaata atatccagcc atattctatt tttttcatct tctgcatcgc caaagtttga
    38101 tgttctaaaa aattgtgaat tgtcataatt caatttattt gtaatatcac ttcgcatagc
    38161 attattaaaa aatgcagttc ctgttgttgc agcaccatca tttatcaaaa caaaaaatcc
    38221 ttgtccagac gctatgtttc cgttaaaagc tccaggacca gtaactgctc ctgataaatt
    38281 atacgtaatg taatctgcag gtgtataatt ataaccgaaa ttataataat aaggatcaac
    38341 cgtagatgca ggtggtgtta agtgtgtcca gatgtttata aatccttcta agtttgtgtt
    38401 agctgttaaa aacttaatag cattaactgc cgaaggatac ggattaccca ctaaattcca
    38461 gttatcgtcc catctagtag tatcaacatt gtttggattt gcaggctcag catcataatt
    38521 taaacccgta gttataccat caccaaaata attacctcta gcaattggca cagtaattaa
    38581 accattattt ggttgaccat aaaaagtaaa aggcaaagtt gttgctgtcg ccgatccatt
    38641 aaatgttctt gcaatatatc ctcttccttc cgtcattgta ggtgtagttg gcactctcca
    38701 attaccaaaa gttcc
//
LOCUS       DAOOBH010000011        34630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_1615_length_34630_cov_8.077918, whole genome shotgun sequence.
ACCESSION   DAOOBH010000011 DAOOBH010000000
VERSION     DAOOBH010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 34630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34630
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1615_length_34630_cov_8.077918"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="PK218_02680"
     CDS             <1..1306
                     /locus_tag="PK218_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPW97444.1"
                     /translation="EIQELKNNQNNFLPFLLLLLSIPYPVWINNIFLTLFILSSIKSY
                     KKKKSYFSITLLLPILLFGLMCLSFFWSIDAKDTLDAIQKELILLLVPLAFIINKDFT
                     SIQRKKAYSYHSYSMVLLAIFFLIRALFRYFLTNDVRVFFYHGENDLDYGLVPKLLNA
                     IHVSVFVAIGFFYFISKEIKSKFDYCASALLFGFILLLSSKNIIIIVILLSLLHFFYF
                     SKSSHKLRLRNLIVFVILVALIFSFGRIKNRFLSEFQENSSESISTNVIEGIPNGVHF
                     VSVKEAWQNKTFTPNDYFPGTAFRVYQFRIFTELFSENNVFFTGFGLNASYPKIEEKA
                     TQYNLYKGNNDETGYQNKNFHNQYIQVFAELGVFGFIILIIILLINLKNAFKTKDFVH
                     FAFAILMISLFLTESFLWRQRGVVFFTLFYCLFNSNVSKKIE"
     gene            1313..2296
                     /locus_tag="PK218_02685"
     CDS             1313..2296
                     /locus_tag="PK218_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW97445.1"
                     /translation="MKRILITGAAGFLGSHLCDRFINEGYFVIAMDNLITGDLKNIEH
                     LFKHQNFEFYHHDVTKFVHVPGQLDFILHFASPASPIDYLKIPIQTLKVGSLGTHNLL
                     GLARVKNARILIASTSEIYGDPLVHPQTEEYYGNVNTIGPRGVYDEAKRFQESITMAY
                     HTFHGVETRIVRIFNTYGPRMRLNDGRVIPAFIGQALRGEDLTIFGDGSQTRSFCYVT
                     DQVEGIFRLLHSDYVYPVNIGNPDEITIKDFAEEIIKLTGTNQKVVYHDLPVNDPMQR
                     KPDTTKAKEILGWEAKVKRSEGMKLTYDYFKSLSSEELLKEEHKDFKGFIH"
     gene            2304..3668
                     /locus_tag="PK218_02690"
     CDS             2304..3668
                     /locus_tag="PK218_02690"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HPW97446.1"
                     /translation="MLTHKGRYSKYIRPISILFDLMVITSLSLIFFDGLKINFKYYLF
                     FQTISWISIALIVKYYEVFRFTTPVEIISKLIKQFSIFLLVVIAYFPFSKVSIFSGKA
                     TAIFMTISFFIIITFKYFLFYYLKKYRLSTGSNFRNAVIIGYTAESIKLKEVFENRKD
                     YGYRFFGFFSDKKQNPDIKGKIEDLKNFTILNKIDEIYCSLNEISNEKLKELVEFADN
                     NDKTIKFIPDSKQIYSKNLKIDYYELFPVLSLQKTELHDPTVKGLKRTFDILFSVFII
                     VFVLTWLMPVIALLIKLESKGPVFFKQGRPGLDEKEFHCYKFRSMKLNVKTEQEASRN
                     DPRVTKIGRFMRKTSIDELPQFFNVLLGDMSVVGPRPHLWSQNKAYANKIKKYMVRHY
                     VKPGITGLAQVKGFRGEIETEEDMVNRIKFDVFYIENWSLLMDLKIIFQTVINIFKGE
                     EKAY"
     gene            complement(3665..4849)
                     /locus_tag="PK218_02695"
     CDS             complement(3665..4849)
                     /locus_tag="PK218_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW97447.1"
                     /translation="MDEILIISNYYPPEKGAAANRIEQLALKLHQKNYNVTVLCPLGN
                     YPKGKLFPEYNGKFSVTENRDNVTVKRLWIYPSVSKNLVVRLLSVLSFSISLFFYLLF
                     KKTPKKIVVQSPPLLLSFISVLVLSIKNKKIILNVSDLWPLAAIELKAVKENSFSHKF
                     SLSLERYIYENSTLILGQSEEIITHIKTIFPEKNCYLYRNFPEHHTSELVIKSSENQP
                     IKIFYAGLLGVAQGVLELCQKIDLKELNVEFHIFGDGAEREQLEIFISNQKEQKIFFH
                     GMVERKKLHEILKSFDVAIVPLKTRIYGSVPSKIFEYTALGFPILYFGGGEGGTIVEE
                     NKLGWVAEVENFQELSEKIIEISKLTHEQLNSIKNKVFKASKLSFNLDLQINDLIEIG
                     VF"
     gene            complement(4849..5937)
                     /gene="wecB"
                     /locus_tag="PK218_02700"
     CDS             complement(4849..5937)
                     /gene="wecB"
                     /locus_tag="PK218_02700"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPW97448.1"
                     /translation="MKITIVAGARPNFIKIAPIIKAIQKKQSVNNKLSFRLVHTGQHY
                     DKNLSDTFFEELNIPHPNSNLEVKSGSQAQQTAAIMVAFEQELIENPCDLVLVVGDVN
                     STMACAIVAKKMNLKVTHVEAGIRSGDMTMPEEINRIVTDSITDYFFTTSTWAGENLM
                     KYGAEASNIHFVGNVMIDTLYQNLDRISAPNFWNEFQLEKGNYIILTLHRPSNVDEEH
                     SLIDLLKGIDKMVGDKKVIFPIHPRTKAILGEQKLELKNILMVDPQGYLNFMFLIKNS
                     FAVITDSGGISEETTVLGIPCFTLRTTTERPETITIGTNNLIGISIENLEKVFGEFLQ
                     NGPRKAGIPELWDGKASERIIDILLSKK"
     gene            complement(5944..7257)
                     /locus_tag="PK218_02705"
     CDS             complement(5944..7257)
                     /locus_tag="PK218_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HPW97449.1"
                     /translation="MLKLFNLSLIFKGFPMKEAKSELSNILALSEENFEKYALKKRNE
                     IVKYHLENNKSYQDFVGSTSFENWNELPIMTKKQFQKPLMERLSHGFTAKNIYVNKTS
                     GSSGDPFIFAKDKFCHALTWANIIYHFGWFGIDFNTSLQARFYGIPLDFIGNTKERIK
                     DVLSNRFRFPIFDLSDVFLEKVLLKFKRKNFAYINGYTSSIVLFAKFLQKKNIVLTSI
                     CPTLKCCVVTSEMLFEEDKVLLEKQFGVRVINEYGASELDLIAFQNITGEWQVNSETL
                     FVEILDEKNNVLPAGKEGRIIITSLYNKAHPFIRYDIGDVGILDEKSTFRKPILKNLI
                     GRTNDVALLPSGKKSPGLTFYYITKSIIEDDGNVKEFVVKQTKINTFDIEYVSEKKLT
                     ENQIETIKTAIEKYLEKGLIFNFKRKEKLERTKSGKLKQFVSLIK"
     gene            complement(7314..8627)
                     /locus_tag="PK218_02710"
     CDS             complement(7314..8627)
                     /locus_tag="PK218_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97450.1"
                     /translation="MKKILLSLSFLLASFATNAQSWVEQASGFNDASRGISQFSIVNA
                     NTVWALAYNGADTTVNIQEFTKTTDGGANWSPGIIDLGDSLLEINNIIAVDETNAWVS
                     ALIPADGNGVVFRTYDGGLNWIQQNSLGFQTSGESFINGVHFFDANNGLSYGDPEGGE
                     FEMYTTTDGGENWTRVPGANIPNPLSGEYGYNGGNVFVGSTCFLVTNKGRILKSTDMG
                     LTWSVSQAPVTDFGSAAQSARLSFSSATNGCMLKTIGTNYTFYTTTNGGTSWSAGTPF
                     TGLYRVLTYVPGTNTIVATSAATAASGSAYSSDNGVTWTSIDSGAQRGIAAFFNGSTG
                     WCAGFNQDASTGGVFKLSGTLSNNQFTSTKFSVYPNPSSSNITISSELDSYKLRVIDV
                     TGKVMINKELNGLENNIDVSSFSNGLYFFEINAGNNTETIKIIKN"
     gene            8745..10019
                     /gene="purD"
                     /locus_tag="PK218_02715"
     CDS             8745..10019
                     /gene="purD"
                     /locus_tag="PK218_02715"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPW97451.1"
                     /translation="MIILLLGSGGREHALACKMQQSLKCSKLFVAPGNAGTSQIATNV
                     SLNILDFVAIKKFVISEKVEMVIVGPEDPLVHGIYDYFKNDAQLNSIPLIGPSLVGAQ
                     LEGSKEFAKEFLVKHNIPTAAYDSFTVETVEKGCVFLESLQPPFVLKADGLAAGKGVL
                     IIQDLEEAKTELRNMLVHAKFGTASSKVVIEEFLDGIELSCFVLTDGKNYKILPTAKD
                     YKRIGEGDTGFNTGGMGAISPVPFADAILLEKIETRIVKPTIEGLQKDGIEYKGFVFI
                     GLINVKEEPIVIEYNVRMGDPETEVVMPRIKSDLVELFQAVAHQKLDEVILEIDERSA
                     STIMVVSGGYPQEYEKGFEISGLENIQDSIAFHAGTKLENGKVVTNGGRVIAVTSYGS
                     DFKEAIKKSYQNIEKLHFDKMYYRKDIGFDLF"
     gene            complement(10011..10241)
                     /locus_tag="PK218_02720"
     CDS             complement(10011..10241)
                     /locus_tag="PK218_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPW97452.1"
                     /translation="MRAFFEAIQSLFVDFLFLPLDALRHLELSSWWLANIISWSLMAI
                     CGYYIVYWCKQLKKHQDNNEEDQDTTAHSFLK"
     gene            10288..11217
                     /locus_tag="PK218_02725"
     CDS             10288..11217
                     /locus_tag="PK218_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HPW97453.1"
                     /translation="MITSLFKKSTPINYSLVVLLLIIFFLLIQFHAESHLSSAFSMFN
                     KISILAILLASFFIVNFIAKKNGLTKNSSYPILFFLLLLCLFPSIFKTPNLIFANFFL
                     LLAYRRLASLQNLNAIKQKLFDASLWICVAALFHFWCILFLFLVYISIIFHASRDYRN
                     WLIPFIALFTTLTIFILAALVFDIAWITYLIETTKTNFQLDYFTNNIQNLALSIYVVL
                     SLYFVFSMIFTLSNKPMILQASYKKMIFGFFIGVIVFLISINKSNAMLVFTFLPLAIL
                     CTNNIEYSQNKTYQEIVLGLFILFGFFSFFSQL"
     gene            complement(11212..11865)
                     /gene="upp"
                     /locus_tag="PK218_02730"
     CDS             complement(11212..11865)
                     /gene="upp"
                     /locus_tag="PK218_02730"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPW97454.1"
                     /translation="MHIHYISEENSILNHFLAQIRDINIQKDSMRFRKNIERIGEIMA
                     YELSKTLEYKEITVQTPLGIKHTTTVAEPVVLCSILRAGLALHTGFMNFFDNAENGFV
                     SAYRHHYDNDDKFEILVEYQAAPSFEEKNLILIDPMLATGHSMVAVLNKLHLEQKPKE
                     IHIAVVIAAPEGIDYLQKNLPKNCHLWVAALDERLNEKNYIIPGLGDAGDLAYGSKL"
     gene            11966..12571
                     /locus_tag="PK218_02735"
     CDS             11966..12571
                     /locus_tag="PK218_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HPW97455.1"
                     /translation="MFSEFAYPIFEQSIKEYHVIDDVYQPTKNPFEKGSIEYLLYAKN
                     WVDTVQWHYEDIIRDPNIDPVAALDLKRKIDASNQVRTDMVEYIDSYFLQKHKDVQVK
                     PDAKINTESPAWAIDRLSILALKIYHMNEEATRVDASPEHRVKCQEKLNVLLDQKNDM
                     FVSINDLLTDIENGDKFMKVYKQMKMYNDDDLNPVLYLNKK"
     gene            12626..13624
                     /locus_tag="PK218_02740"
     CDS             12626..13624
                     /locus_tag="PK218_02740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPW97456.1"
                     /translation="MGDVAMTVPVIRAFVEQNPNVKITFVSRPFFKPFFDGIPNVSFF
                     AVDVKKRHKGFLGLLKLYSDLKKLNIDAVADLHNVLRSQIIRILFFLSGKKVAATDKG
                     RAEKKALTRAENKIFQPVKSMVQRHVDTFNTLGFNVDLSNPKFPEKAVLSKEIIEILG
                     EKQNFNWIGIAPFAQYESKVYPLDLMQKVIDELALNASNKIFLFGGGNKEIELLNQFS
                     KNKENVINLAGKIPFQHELQLISNLDVMLSMDSGNAHIAAMLGVKVITLWGATHPFAG
                     FAPFNQPLENCLTSDREQFPLLPTSVYGNKKVAGYEDVMRTIAVEKLIEKINSNLE"
     gene            complement(13666..14718)
                     /locus_tag="PK218_02745"
     CDS             complement(13666..14718)
                     /locus_tag="PK218_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HPW97457.1"
                     /translation="MSTFYKLNIKEVTQETPSAISVAFTVSPELKPAYQFIAGQYVNL
                     KLTLDGEEIRRAYSICSSPNSDELRIAIKSVKDGHFSKFANENLKIGDVIEVGQPEGR
                     FTFEPSIERQKNYAAFAAGSGITPIMSIIQSVLENEPKSTFILVYGNKTPEETIFHKQ
                     LHDLHLKYVARFFVHYVYSKVNVEGELFGRIDKSVVNFVLNNKHKEKEFEKFYLCGPE
                     EMINRVSDVLKEHNIADKNIKFELFSTSSSENNLISSSEGHSKITIMVDDEETTFEMS
                     QKQTVLEAALKHGIDAPYSCQGGICSSCLARITSGTAVMAKNSILTDKEIASGLILTC
                     QAHPTSSEIYVDYDDV"
     gene            14837..15172
                     /locus_tag="PK218_02750"
     CDS             14837..15172
                     /locus_tag="PK218_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW97458.1"
                     /translation="MKNSQLYKGSLNTIVMKLLEENGRMYGYEITQKVKDITKGELKI
                     TEGALYPALHKMEAEGILEVEVENVDNRLRKYYKLTQKGTTETVNKLAELEEFIKNMQ
                     SIVQPKLGY"
     gene            15175..15867
                     /locus_tag="PK218_02755"
     CDS             15175..15867
                     /locus_tag="PK218_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97459.1"
                     /translation="MKLSTEQIEKLYQFTRQHYVEYYDLQTELVDHLANGIEEQWQEK
                     PKLSFDEALQVEFKKFGVFGFMDVVEKRTAAMNKRYNKMILNHLKVYFRIPQIIGTIA
                     AVGIVFYLLKMSLVSVFTMSILGLISIGFLFFSLFYLVRKRKKYEAKTGKKWMLKEII
                     FGHSTSLGLIQFPIQLFLQIDEPLVDIWLIIFSVFFVLIFICEYIIIFEIPSKAEDYL
                     KQTYPEYEFSES"
     gene            15923..17392
                     /locus_tag="PK218_02760"
     CDS             15923..17392
                     /locus_tag="PK218_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HPW97460.1"
                     /translation="MIKHFISLEWKSFLRSASFGTNLALKIFMALGALYFAAMFVGLG
                     IAVYFLIEDAGLKPFETVNKFLIYYFTADLVIRYFLQTTPVLNIKPLTILNIKKNTIV
                     NFVIGKSALSFFNFLHAFFFIPFSIVLVKEGFNPMQVVFWHIGVFSIIYSMNFINVLV
                     NKKDSIFYVVLTIVGGFGLAQYYGWFDVTVYTSPFFQGMYDTSYLFLFPIAALVLLYY
                     FNFKFFKNNLTLDTGLAVKSKEATTENLTFLDQFGTLGTFLKNDIKLLKRNKRSRTTV
                     IMSVVFIFYGLLFFTNSIEAYNNPAMHVFAGIFVSGGFLFTFGQFVPSWDSAYYQLMM
                     SQNIPYREYIKSKWWLMVIGTVVSTILASFYLYFGWHIYLLIVVGAIFNIGVNSHLVM
                     LGGAFVKTPIDLSSSKQAFGNKQAFNMKTMLISLPKLLLPMALYAAGYYIFSPEVGLL
                     FVAGAGVLGFAFRNKVFTMIEKIYKTEKYATISAYKQTN"
     gene            17425..18123
                     /locus_tag="PK218_02765"
     CDS             17425..18123
                     /locus_tag="PK218_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW97461.1"
                     /translation="MIQVNNLSKIYTNGKKVLNIANLEIPKGQSFGLVGNNGAGKTTF
                     FSLLLDLIEPTTGSIKNNEVIVNQSEAWKPFTAAFIDESFLIGYLTAEEYFYFIGDLR
                     GQNKADVDALLKKHEQFFNNEILNNKKYLRDLSKGNMKKVGIIAALIGNPEVVILDEP
                     FANLDPTTVSRLKKIIKELADNPEVTVLVSSHDLVHTVEVCDRIVALNLGEVVKDIQT
                     SPETLKELETFFAV"
     gene            18264..21119
                     /locus_tag="PK218_02770"
     CDS             18264..21119
                     /locus_tag="PK218_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW97462.1"
                     /translation="MKTKITKRILFFGFLFFLIACSTKKNSFLSRNSHALSSKFNILY
                     NGDLALEQGLLELKSQYQDNFWEILPIERMQIKEEQLLPNETKNPNFERAEEKATKAI
                     QKHSMNIQGSEKNPQMDEAHLLLGKARYYDQRFVPALEAFNYVLYKYPKSSKIYEVKI
                     WREKTNMRLENDQLAVNNLRKLLKEIKFKDQIFADANAVLAQAFLNLEVKDSAVAKLK
                     LAREFTKSNEEKSRYDFILGQLYENLGHKDSAYASYQKVIDMHRKAAKQYTIHAHINQ
                     VLQQEYKKGDTLLFLKKWNKLLADRENRPYLDALYHQKAKFYEGQENFEAAKKNYNLS
                     LKKKSQDQYLMASNYRNLATIYFNDAKYATAGKYYDSTLVNLKPRTREFNAIKKKREN
                     LEDVIKYEAIAQKNDSIISLYKMSSSDKVAYFDKYIEKLKKEDAKKLKELEKQEQIKV
                     NSESFGGDESQSINAAKMAANVPKPNLFTGSKTDFYFYNPTTIAYGKKEFTKIWGKRK
                     LVDNWRVSSLLTKEDAKGNDEKDAKENAVTSKEKDGKENSDENPAYTSDFYISQLPTS
                     QKVIDSLSKERNFAYYQLGVIYKEKFKEYQLAVNKLEQLLKNNPEERLILPSMYNLYK
                     LYEILDKNKALAMKARIIAEYPESRYAQILSNPQDQLADDNNSPKAVYEALFKQYQER
                     NFKEVLVKIDEAIERFTGDEIAPKLELLKANLIGKLKGLDEFQQALNYVALNYPNVEE
                     GKTAEKTISISLPKLRALQLGKYESTNWKILYQSKDFDDKNTKALREKITKFIKERSL
                     DKLTISLDVYTMTDNFVVIHGMKSEDYAKGIASILKEFKEYKVQDTPIIISTENYSVV
                     QIKKNLQDYIDGKLVDNPKEPNWDGSIEKAPVAQQEPPKQEEKPRNTKAEQKAIQNQK
                     EELKGAQSIQNDKKIKATQNEENTFGPPPAPQMPNGK"
     gene            21128..21532
                     /locus_tag="PK218_02775"
     CDS             21128..21532
                     /locus_tag="PK218_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPW97463.1"
                     /translation="MFEKRSKPYTDLLGKTNRIVEGTTINGNVVSATDIRLDGQLIGN
                     LQSKGKLVIGPTGNIKGDVICRNADIEGLFEGKIQVQEILNIKSKAHIKGDVICGKLS
                     VEPGAEFSATCAMKPQVKNITTDGKSEQEKIA"
     gene            21501..21734
                     /locus_tag="PK218_02780"
     CDS             21501..21734
                     /locus_tag="PK218_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPW97464.1"
                     /translation="MENQNKKKSPNKWLVLINIPIQMGIVIFLFAYLGKYLDTTYPNT
                     HSVYVKVLTLSGVAIAFYNLNRQLKKLNDSDKQ"
     gene            21731..22117
                     /locus_tag="PK218_02785"
     CDS             21731..22117
                     /locus_tag="PK218_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97465.1"
                     /translation="MNFKTQKPVILAIVFYTIHKVIFLVAFPKEEINTFVYPLELLYL
                     FFGFSSVLIVFLLVKINQKNINNVGYTFLLVTSVKMALAYFFLQPILNSKTEFIKMDK
                     MNFFVIFIVFLIIETSVTIKMLNKKQ"
     gene            22234..23397
                     /gene="atpB"
                     /locus_tag="PK218_02790"
     CDS             22234..23397
                     /gene="atpB"
                     /locus_tag="PK218_02790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPW97466.1"
                     /translation="MVISKKPRLFIYAALVALLPLFGLANPNDSTTVSHQVAHADKTT
                     HENTEGLTKEQLKVKETKEFIRHHLLDSHDFHLFSYGKDEAGHENHVSFPLPVILWDN
                     GIHFFMSSKFEHGHAAAESKGNYYAINHHNGQIYKTDAAGTFIENDKGKIANDKPIDL
                     SITKNVSMILITGLIMFLLFAGLGKSYKKNGSIAAGAGRFFEPIILYIRDEIAIPNIG
                     VKYYRKYMSFLLTIFFFVWFLNMFGLTPLGVNVTGNIAITAALAIIAYIITTFTAKKD
                     YWKHIFWMPGVPVPMKFILAPIELLGTFIKPFSLMIRLYANMLAGHVVLMSLIALMYS
                     ANHFIGSPLALLLSFVLTILEVLVALLQAYIFTMLAALYFGSAVEEHHHEEAH"
     gene            23493..23684
                     /gene="atpE"
                     /locus_tag="PK218_02795"
     CDS             23493..23684
                     /gene="atpE"
                     /locus_tag="PK218_02795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPW97467.1"
                     /translation="MEIPRIIGAGLVVIGAGIGIGRIGGSAMDAIARQPEATGKIQTA
                     MLIAAALIEGIGFAAIFAT"
     gene            23770..24270
                     /locus_tag="PK218_02800"
     CDS             23770..24270
                     /locus_tag="PK218_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPW97468.1"
                     /translation="MEELFGQFSLGLFILQFVLFVALVFLLKKFAWKPILDAVNERED
                     GIKNALLSAENAKKEMQNLKSDNEKLLADARLERDAMMKDAREIKEKMINDAKTEAQV
                     AGEKMIAQAKAAIESEKNAAMAELKAQVSNLSLDIAEKVLKSELSNKESQTKLVEKML
                     GDVKLN"
     gene            24270..24803
                     /gene="atpH"
                     /locus_tag="PK218_02805"
     CDS             24270..24803
                     /gene="atpH"
                     /locus_tag="PK218_02805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPW97469.1"
                     /translation="MDMSRAAIRYAKAILEIASTSGNTAKVNNDMKAITEVVSGNKEL
                     SDFLASPIITSDIKMNALSEVFGTVENETKSLFRLLQENKRFEILLPIAEQFNAQYDV
                     LNGVEVAVVTTAIPMDAALETQVLAKIATISDKKITLQNTIDPSIIGGFILRMGDKQY
                     NASVANRLQELRREFSN"
     gene            24827..26404
                     /gene="atpA"
                     /locus_tag="PK218_02810"
     CDS             24827..26404
                     /gene="atpA"
                     /locus_tag="PK218_02810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPW97470.1"
                     /translation="MAEIKAAEISAILKKQVEGFQSGATLEEVGSVLQVGDGIARVYG
                     LSNAQYGELVQFENGLEAIVLNLEEDNVGVVLLGPSTGIKEGSTVKRTQRIASLNVGE
                     NIVGRVVNTLGEPIDGKGPIGGDLYEMPLERKAPGVIFRQPVTEPLQTGIKSIDAMIP
                     VGRGQRELVIGDRQTGKTTVCIDTILNQKEFYDAGQPVYCIYVAIGQKASTVAGIAKT
                     LEEKGAMAYTTIVAANASDPAAMQVYAPMAGAAIGEYFRDSGRPALIIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERACKVIADDDIARNMNDLPDSLKPIVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLDGDLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVSGTLKLDQAQFRELEAFAKFGSDLDAVTLNVIEKGRRNVEILKQAVN
                     DPYTVEDQVAIIYAGSKNLLKTVPVNKIKEFEKDYIEYLNAKHRDTLNALKAGKLDDN
                     ITDVLEKAAKEISAKYN"
     gene            26477..27337
                     /gene="atpG"
                     /locus_tag="PK218_02815"
     CDS             26477..27337
                     /gene="atpG"
                     /locus_tag="PK218_02815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPW97471.1"
                     /translation="MANLKEIRNRISSVSSTMQITSAMKMVSAAKLKKAQDAITAMRP
                     YAEKLTELLQNVSATLDGDAGGEFTSQREINKVLVVAITSNRGLCGAFNTNVIKQAKL
                     VAESYTGKQVDIFAIGKKGNDILRKTYNVVDNKSEVFDQLSFDNVADIADALTEKFTS
                     GEYDKIEIIYNQFKNAATQIVQTEQFLPLAPIVSDVPVSTGDYIFEPSKEEIVLTLIP
                     KSLKTQLYKAIRDSFASEHGARMTAMHKATDNATELRNQLKLTYNKARQAAITNEILE
                     IVGGAEALKG"
     gene            27439..28914
                     /locus_tag="PK218_02820"
     CDS             27439..28914
                     /locus_tag="PK218_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPW97472.1"
                     /translation="MSIYKNLFKQTMIYGLATVLPRMFSFILVPLYTDLLPKAEYGQI
                     TVIFAYMIFFNVILAYGMETSFFRFYNKEDDKKKVIETSMVTIFWTTILFLAGALTFR
                     NDIAKFIEIDVQYVTYSIWILVLDALVIIPFSKLRAIQRPSFYAFLKISNVVVNLSLN
                     VFFLVYLPKIVASQPSGFLSSIYFENFQIGYVFISNVIASLLTFVLLSKDYFTLNWKF
                     DVQLWKQMMKYGMPILVSGVAFAINEQFDKILLQKLLPPNIAEAEVGVYSACYKLGLF
                     MVLFRTAYTLGIEPFFFSHAKNENAPQTYATITKYFVIFGSFIMLNVIVFADLFKQLM
                     IRDASYWEAMKVVPLIILANFFLGIYTNLSVWYKLIDKTYIGALISLVGAAVTLVLNF
                     WLIPKTGYFGGYYGSAIATIAAYGSMMLLSYYLGKKQYPIPYEMKKIGLYLMLSVAFS
                     IVSFYFFRENYLVGIPLILIFLYFIYYNEKETLATIVKRKK"
     gene            28911..29345
                     /gene="dut"
                     /locus_tag="PK218_02825"
     CDS             28911..29345
                     /gene="dut"
                     /locus_tag="PK218_02825"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPW97473.1"
                     /translation="MTIKIINKSQHELPNYETIASAGMDLRANLEAPITLKSLERTIV
                     KTGLFIELPIGFEAQVRPRSGLAAKKGITVLNAPGTVDADYRGEIGVILVNLSSEDFT
                     IENGERIAQLIIAKHERAEWMAVETLSETSRGEGGFGSTGVK"
     gene            29720..30058
                     /locus_tag="PK218_02830"
     CDS             29720..30058
                     /locus_tag="PK218_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97474.1"
                     /translation="MATGFHLGNYLTKLIDTKRLRRKEITQQMGVQYSAFYGYEGRPS
                     LQFYIVLRLCHAMKYNIFLDVANMLPPEYDYDKKSSTGKDNIIAQLEEENKKLKWEND
                     LLKEMMIKKG"
     gene            30136..31146
                     /locus_tag="PK218_02835"
     CDS             30136..31146
                     /locus_tag="PK218_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPW97475.1"
                     /translation="MKIIVPMAGRGSRLRPHTLTIPKPLIPIAGKPIVHRLVEDIAGV
                     LNQDIEEVAFIIHESFGKKVEDELIAIAEKLGAKGTIYYQNEALGTGHAIMCAKDSLT
                     GSAVIAYADTLIRAKFDLDQTADSVIWVKQVEQPEAFGVVNLNDNNEIIELVEKPQEF
                     VSDLAVIGIYYFKDVAVLKNELQSVLDNNIIHGGEYQINDGIKQMMAKGMKFVPGEVD
                     EWMDCGNKDVTVETNSRMLGFLLNDGEKLISDVVRVENSTVIEPCFIDDDVVLINSIV
                     GPNVSLGKGTHLTNVKIQNSLVQTHAHIRNAKLDNAMIGNHATFDGEFTSISIGDYSV
                     LE"
     gene            31189..32538
                     /locus_tag="PK218_02840"
     CDS             31189..32538
                     /locus_tag="PK218_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biosynthesis protein"
                     /protein_id="HPW97476.1"
                     /translation="MKKIVLHRFLFGILFIPTILMAQTEPEDVASITNEFENNFFESL
                     KQKGIENYDKAIESLDKCLVLEPDNPAVLFELGKNYLAQKKYPTAYSYFEKATQIDAK
                     NRWAWVGMYDVCHETQDYNKAIPIVEKLITFKDSYKEDLVSLYMNTQQFDKALELINN
                     LNETVGKSEKRELYKADILRDAKYQSAEKFNLLDQIKKFPKEESNYIALIYMYSQSNQ
                     EEKALEIAEKLEKEIPTSDWAQVSLFKFHLNNNDGAQAVKAMNIVLPSAKIDSKIKHR
                     MLNEFLIFATNNPQFEPDLDKAIAYFDNDKNVDVAKEIGKFYFMKSDWAKAKKYFELQ
                     TKTHSDDIESQKLLLQSYVELQDFKAVAKKADDLTQYYPTQPDLYYYAGLAQNQLGNF
                     KKAKEALEAGLDFIIDDVSLEINFYLQLSETFNGLDDVKKKESYFAKANDLSKKQKK"
     gene            32535..33308
                     /locus_tag="PK218_02845"
     CDS             32535..33308
                     /locus_tag="PK218_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HPW97477.1"
                     /translation="MKKIIFVVSICFFAMSCKSKKALISEGIATEKIKATEIIKNHYN
                     NKNDFKTLYIKASARYEDPKNANSVTAEVRIQRNEKILVSIRFLGITMAKALITPSNV
                     YYYEKINGTYFEGDYELLSQFLGTELDYTKVENLLIGEAIDDLNKEKFVALIVDSFYK
                     LNNVNTNEIDKTFYFEADNFLVKKEEIIQNSKNRKVTIEYPNFQKVGEYMLPAAIEIN
                     AEQEKGKTAISIKYNTINLNDELSFPYSVPDGYERIFIK"
     gene            33327..34574
                     /locus_tag="PK218_02850"
     CDS             33327..34574
                     /locus_tag="PK218_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPW97478.1"
                     /translation="MTKHILTFLFFCLALPMWSQLSKQEQLEERKAKIQLEIQERQQL
                     LNSMKSKEKTVVSQLMIQKDKINLKEQLIKTTEKQTKLLNDDIYRNQLKINQLNKELE
                     VLREDYAEMIRKSYKSRSEQSRVMFLLSSENFLQAYKRIQYMKQYNNYRKMQADELKA
                     KTTKLKSTTEEIKVQKTEKEKLIVENEKEKQELEKEKQEQEKIAKQIQKDKKNIVAEI
                     KKKQQETKKIDAQIQKLIRDAIAEANRKTAKATSSKTKTTAEKKAIENSVNIVLTPEG
                     KLISDNFKANKGRLPWPVEKGAITLGYGEQPHPVFRTLTVYNSGIEIATENGADAKAV
                     FDGEVSQVQLTSPQKKTVIIKHGDFFTVYNNLSTVNVREGDKVSARETIGKIRTDGDG
                     KTILKFMISQNTTYFNPTPWLSK"
BASE COUNT        12317 a         5381 c         5801 g        11131 t
ORIGIN      
        1 agaaattcaa gaattaaaaa acaatcaaaa taactttctt ccatttttat tgttactgct
       61 ttctatacct tatcctgttt ggattaacaa tatttttttg acgttattta ttctttcaag
      121 cataaaaagt tataagaaaa aaaagtctta tttttcgatc actttgctat taccaattct
      181 tttatttgga ttaatgtgtt tgtctttttt ttggagcatt gatgcaaaag atactttaga
      241 cgccatccaa aaagaattaa tactactatt agtaccattg gcatttatta taaataaaga
      301 tttcacatca attcagcgta aaaaagccta cagttatcac agctattcaa tggttttatt
      361 agccatattc tttttaatta gagcactttt tagatatttt ttaacaaacg atgttagggt
      421 gtttttctat cacggagaaa acgatttaga ttatggttta gttccaaaat tattaaacgc
      481 cattcacgtt tcagtctttg ttgccattgg ttttttctat tttatttcaa aagaaataaa
      541 atctaaattt gattattgtg caagtgcttt actgtttgga tttattctat tactttcttc
      601 taaaaacatc attataatag taattctatt gtctttactt catttcttct atttttcaaa
      661 atcatcacac aaattacgcc ttagaaattt aattgttttt gtaattttgg tagctttgat
      721 tttctccttc ggaagaatta aaaatagatt tttatctgaa tttcaggaaa acagtagcga
      781 aagcattagt accaacgtaa ttgaaggaat tcctaatggt gttcattttg taagtgttaa
      841 agaagcttgg caaaataaaa ctttcacacc taatgattat tttccgggta cggcatttag
      901 agtgtatcaa tttagaattt ttaccgaatt attttcagaa aataatgttt tttttacagg
      961 ttttgggtta aatgcgtcct atccaaaaat agaggaaaaa gctacgcaat acaatcttta
     1021 taaaggaaac aatgatgaaa caggttatca aaataaaaat tttcacaacc aatatattca
     1081 agtttttgcg gagttaggcg ttttcggttt tatcatatta attataatac tccttattaa
     1141 tctaaaaaat gcttttaaaa caaaagattt tgttcatttt gctttcgcaa ttctaatgat
     1201 aagtttattt ttgaccgaat cgtttttatg gcgacaaaga ggagttgttt tttttactct
     1261 tttctactgt ctatttaatt caaacgtttc taaaaaaata gaataaaaaa ctatgaaaag
     1321 aatattaata actggagctg caggatttct gggttcgcac ttgtgtgatc gttttattaa
     1381 cgaaggctat tttgttatag caatggataa tttgattaca ggcgatttaa aaaacatcga
     1441 acatcttttc aaacatcaaa attttgaatt ttatcatcac gacgtaacca agtttgtaca
     1501 cgttcctgga cagttagact tcatactaca ttttgcttca cctgcaagtc ctattgatta
     1561 tttgaaaata ccaattcaaa ctttaaaagt aggttcacta ggaactcata atcttttagg
     1621 attagcaaga gttaaaaatg ctagaattct aatagcttca acttcggaaa tatatggcga
     1681 tccattagtt catccacaaa cagaagaata ctatggaaat gtaaatacta ttggtcctcg
     1741 tggtgtttat gatgaagcaa aacggtttca agagtcaata acgatggcat accacacatt
     1801 tcatggtgta gaaacaagaa tagttagaat ttttaatacc tatggaccaa gaatgcgctt
     1861 aaacgatgga cgagtaattc ctgcatttat tggtcaggct ttgcgtggcg aagatttaac
     1921 catttttggc gatggaagtc aaacgcgttc cttttgttat gtaaccgatc aagtagaagg
     1981 aatttttaga ctgcttcatt cggattatgt ttatccagta aatattggaa atcctgatga
     2041 aattaccatt aaagattttg ccgaagaaat tataaaattg accggaacaa atcaaaaagt
     2101 tgtctatcac gatttgccag ttaacgaccc aatgcaaaga aagccagata ctacaaaagc
     2161 taaagagatt ttgggttggg aagcaaaagt gaagcgatca gaaggaatga aattaaccta
     2221 tgattatttc aaatcacttt cttctgaaga gttgttaaaa gaagaacata aagactttaa
     2281 aggatttatt cattaattaa gtaattttga ctcataaagg cagatattct aagtacatta
     2341 gacccataag tattctattt gacttaatgg taatcacatc attaagctta attttctttg
     2401 acggtttgaa aattaatttt aaatactatt tgttttttca aaccattagt tggataagta
     2461 ttgctttaat tgtaaaatat tatgaagtat ttcgatttac aactccagta gaaataattt
     2521 caaaactcat caagcaattt agcatctttt tattagtagt aattgcttat tttccttttt
     2581 caaaagtttc catttttagc ggaaaagcaa ctgctatttt tatgacaatt agttttttta
     2641 taattataac cttcaaatat tttttatttt attatttaaa aaaataccgt ttatctacag
     2701 gcagtaattt cagaaatgct gtaataatag gctatacagc tgaatctata aaattaaaag
     2761 aagtttttga aaacagaaaa gattacggtt atcgtttttt tgggttcttt tcagataaaa
     2821 aacaaaaccc agatattaaa ggaaaaattg aagatttaaa aaactttaca attctaaata
     2881 aaatagatga aatatattgt tccttgaatg aaatatcaaa tgaaaaacta aaggaattag
     2941 tcgaatttgc agacaataat gataaaacaa taaaatttat tcccgattca aaacaaattt
     3001 attcaaaaaa tctaaaaata gattactatg aattattccc cgttttatct ttacaaaaaa
     3061 cggaacttca tgacccaact gtaaaagggt tgaaaagaac ttttgatatt ttattttcag
     3121 tatttattat agtttttgtt ttaacttggt tgatgcccgt cattgcactt ttaataaaat
     3181 tagaatcaaa aggaccagtt ttttttaaac aaggaagacc aggattggat gaaaaagaat
     3241 tccattgtta caaatttcgc tctatgaaat taaatgtaaa aacagaacaa gaagcatcta
     3301 gaaatgaccc aagagttact aaaataggca gatttatgcg taaaacaagc attgacgaat
     3361 tgcctcagtt ttttaatgtt ttattaggcg atatgtcagt tgttggacca agacctcacc
     3421 tttggtcaca aaacaaagcg tatgctaata aaataaaaaa atacatggtt cgtcattatg
     3481 taaaaccagg aataacaggt ttggcacaag taaaaggatt tagaggagaa atagaaacag
     3541 aagaagatat ggtgaaccga atcaaatttg atgtttttta tatcgaaaat tggtcattac
     3601 ttatggattt aaaaattatt tttcaaacgg taattaatat tttcaaaggc gaagaaaaag
     3661 catattaaaa aacgccaatt tcaatcaaat catttatttg aaggtctaaa ttaaaactca
     3721 atttggaggc tttaaaaact ttatttttta tcgaatttag ttgttcatga gttaattttg
     3781 aaatttcaat aattttttca cttaactctt gaaaattttc tacttctgca acccatccca
     3841 atttattttc ttcaacaatt gttccgcctt cgccgccacc aaaataaagt ataggaaaac
     3901 ctaaagcagt atattcaaat attttagaag gaacagaacc ataaatccta gtcttaagtg
     3961 gaacaatggc tacatcaaat gattttaaaa tttcatgaag tttttttcgt tccaccatac
     4021 catgaaaaaa tattttttgt tctttttgat tagaaataaa tatttctaat tgctctctct
     4081 cagcaccatc tccaaatata tggaattcaa catttagttc ttttaaatca attttttgac
     4141 acaattctaa aacaccttgt gcaactccta ataaacctgc ataaaaaatc ttaataggtt
     4201 gattttctga tgattttata actaattcag aagtgtgatg ttcgggaaaa tttcggtata
     4261 agtaacaatt tttttcgggg aaaatagttt taatatgagt aattatttct tcggattgtc
     4321 ccaaaattaa tgttgaattt tcataaatat atcgctctaa agacaatgaa aatttatgtg
     4381 aaaaagaatt ttcttttacc gctttaagtt caatagcagc caatggccat aagtctgaaa
     4441 cgtttaaaat tattttctta ttttttatag ataatactaa tactgaaata aaagaaagca
     4501 acaatggtgg cgactgaaca actatttttt taggtgtttt tttgaatagc aaataaaaaa
     4561 acaaacttat tgaaaacgac aaaacagaca gcaatcggac aactaaattt ttactcacac
     4621 tagggtaaat ccaaagtctt tttaccgtaa cgttgtctct gttttcagtt accgaaaact
     4681 ttccattgta ttcaggaaac aatttgcctt taggataatt tcctaatgga caaagaacag
     4741 taacattata gtttttttga tgcaatttta gtgctaattg ctcaattcga ttagctgcag
     4801 ctcctttttc tggaggataa taattggata tgattaggat ttcatccatt atttttttga
     4861 aagcaatata tctattattc tttctgatgc ttttccgtcc caaagttctg gaataccagc
     4921 ttttcttggg ccattttgaa gaaactcgcc gaaaactttt tctaaatttt caattgaaat
     4981 tccaattaaa ttattggtac caatagttat agtttctggt ctttctgtag tagttctcaa
     5041 tgtaaaacaa ggaattccta aaacggtagt ttcttccgaa atgcctcccg aatcagttat
     5101 aacggcaaaa ctatttttta ttaaaaacat aaaatttaaa taaccttgag gatcaaccat
     5161 taaaatgttt ttcaattcta atttttgttc gcccaaaata gcttttgttc ttggatgaat
     5221 tggaaaaatc acttttttat ctccaaccat tttatctatc ccttttaaaa gatcaatcag
     5281 agagtgctct tcgtcaacat tggatggtcg atgcagcgta agaataatat aatttccttt
     5341 ttcaagttga aattcattcc aaaaatttgg agccgaaatt ctatctaaat tttgatacaa
     5401 tgtatcaatc attacatttc caacaaaatg aatatttgaa gcctctgcgc catatttcat
     5461 taaattttct ccagcccaag ttgaggtggt aaaaaaataa tccgtaatgc tatcagttac
     5521 aattctgttt atctcttcgg gcattgtcat atcaccagaa cgaatgccag cttcaacatg
     5581 agttactttt agattcattt ttttggcaac tatagcacaa gccatagttg aattaacatc
     5641 gcctaccact aaaaccaaat cgcatgggtt ttcgattaat tcctgctcaa aggctaccat
     5701 tatagccgca gtttgttgtg cttgagagcc actttttacc tctaaatttg aattaggatg
     5761 aggaatatta agttcttcaa aaaaagtatc acttaaattt ttatcataat gctgcccagt
     5821 atgaaccaag cggaaagata atttgttatt tacagattgt tttttttgga ttgccttaat
     5881 aataggcgct atttttataa aatttggtct tgcacctgca actattgtga ttttcatatt
     5941 tttttatttt atcagcgaaa caaattgttt caattttccg cttttcgttc gttctaattt
     6001 ttcttttcgt ttaaaattga aaattaatcc tttttcaaga tacttttcaa ttgccgtttt
     6061 aatggtttca atttgatttt ctgtcaattt tttttcacta acatattcga tgtcaaaagt
     6121 attgattttg gtttgtttta ccacaaattc tttcacattt ccatcatctt caataatgct
     6181 tttagttata tagtaaaacg ttaatccagg agatttttta ccactaggta aaagtgcaac
     6241 atcattggtt ctgccaatta ggttttttaa aattggcttt ctaaaagtgc ttttttcgtc
     6301 taatattcca acatcaccaa tatcgtatct tataaaagga tgtgccttat tatataatga
     6361 agtgataata attcggcctt ctttcccagc tggcaaaaca ttgttttttt catctaaaat
     6421 ttcaacaaat aaggtttcag agtttacttg ccattctcca gttatgtttt gaaaagctat
     6481 taaatccaat tcagaagcgc catattcatt gattacacgt actccaaatt gtttttccaa
     6541 taaaacttta tcttcttcaa aaagcatttc tgaagttacc acgcaacatt ttaaagttgg
     6601 acaaattgaa gttaaaacaa tatttttttt ctgcaaaaat ttggcaaaaa gaactattga
     6661 agatgtataa ccgttaatgt aagcaaaatt tttacgttta aatttcaata aaactttctc
     6721 caaaaaaaca tcagacaaat caaaaattgg aaaccgaaac cgattgctca atacatcttt
     6781 gattctttct tttgtgttgc caataaagtc taaaggaata ccataaaaac gtgcttgtaa
     6841 tgatgtatta aaatctattc caaaccagcc aaaatgataa ataatatttg cccaagttaa
     6901 agcatgacaa aatttatctt ttgcaaaaat aaaaggatcg ccacttgaac cagaggtttt
     6961 attgacataa atatttttgg cagtaaatcc atgtgataac ctttccatta gtggtttttg
     7021 gaattgtttt ttagtcatta ttggcagttc gttccaattt tcaaaagaag tgctgcccac
     7081 aaaatcttga taggatttat tgttttctaa atgatattta acaatttcat ttcttttttt
     7141 aagcgcatac ttttcaaaat tttcttcaga taaagctaaa atgtttgata attcagactt
     7201 tgcttccttc atcgggaaac ctttaaaaat aagggataaa ttgaaaagct tgagcataat
     7261 aaaataaaaa agggctaacg aaaagtcagc ccttaaaaat actattttaa atattagttt
     7321 ttaataattt tgatagtttc ggtattgttt cctgcattta tttcgaagaa gtacaaccca
     7381 ttagaaaaac ttgaaacatc tatattgttt tctaaaccat ttaattcttt gttaatcata
     7441 actttacccg taacatctat cactcttaat ttataagaat ctaattctga agagatagtt
     7501 atatttgaag aagatgggtt aggataaaca ctgaatttag tagaagtaaa ttgattatta
     7561 cttaaagtac cacttaattt gaatacacca ccagttgatg catcttgatt aaaccctgcg
     7621 caccaacctg ttgagccatt aaagaatgca gcaatacctc tttgagcacc tgagtctatt
     7681 gaagtccatg tcacaccatt atcagaacta taagcagagc cagaagctgc agttgcagcc
     7741 gaagtagcta caatagtgtt agttccagga acataagtta atactctata taatcctgtg
     7801 aaaggtgtgc ctgcagacca tgatgttcca ccgtttgttg ttgtataaaa agtataatta
     7861 gtaccgattg ttttaagcat acaaccattt gttgcagagc taaaagacag tctagcactt
     7921 tgagctgcag aaccaaaatc tgttactggc gcttgagaaa cagaccatgt caatcccatg
     7981 tcagttgatt ttagtattct acctttgtta gttactagaa aacaagttga acctacaaaa
     8041 acgtttccgc cattgtatcc atattcacca ctcaatgggt ttggtatgtt agctccagga
     8101 actcttgtcc aattttcacc gccatcagta gtagtgtaca tttcaaattc accaccttca
     8161 ggatctccat atgataaacc attattagca tcaaaaaaat gaacgccatt tataaaagat
     8221 tcaccagaag tttgaaagcc aagagagttt tgttgaatcc aatttaaacc accgtcgtag
     8281 gttctaaaaa caactccgtt tccgtcagct ggaattaaag cacttaccca tgcattagtt
     8341 tcatcaacgg caataatgtt gttaatttct aaaagcgaat ctcctaaatc gataatccct
     8401 ggtgaccaat tggcaccacc gtctgtagtt ttagtgaatt cttgaatgtt tacagtagta
     8461 tcagcaccat tataggctaa tgcccacacg gtattagcat tcacaatgct aaattgagaa
     8521 ataccgcgac ttgcgtcatt aaaaccacta gcttgttcaa cccatgattg ggcatttgta
     8581 gcaaaactag ctaataagaa tgataaagaa agtaaaattt ttttcatatt ttttttattt
     8641 atgtgtttga ggagtctaaa ttagaaaaaa aaatgatgca taccaaatta tctgtttatt
     8701 tttgtaaaag atttaacatg tatattagtt ttaaaacaca cacaatgata attttactac
     8761 ttggttctgg tggaagagaa catgcactag catgtaaaat gcaacaaagt ttaaaatgtt
     8821 ccaaactttt tgttgcacca ggaaatgcag gaacatcaca aatagcaact aatgttagtc
     8881 taaatatctt agattttgtt gccataaaaa agtttgtcat atctgaaaaa gtagaaatgg
     8941 ttattgttgg tccagaagat cctttggttc acggaattta tgattatttc aaaaatgatg
     9001 cacagctaaa ctccattcct ttaattggac cgtccttagt aggggcgcaa ttagaaggca
     9061 gcaaagaatt tgctaaagag tttttagtaa aacacaacat tccaacggcg gcatatgata
     9121 gtttcacagt agaaaccgtg gaaaaaggtt gtgttttttt agaatcacta caaccaccat
     9181 ttgttttaaa agcagatgga ttagctgcag gcaaaggggt tttaattatt caagatttgg
     9241 aagaagcaaa aaccgaattg agaaatatgt tggttcacgc taaatttgga accgcaagct
     9301 caaaagtggt aattgaagaa tttttagatg gaattgaatt aagttgtttt gtgttgaccg
     9361 atggtaaaaa ttataaaatt cttccaactg caaaagatta caagcgaata ggtgaaggcg
     9421 atacaggatt taacacaggt ggaatgggag cgatttcccc tgttccgttt gcagatgcta
     9481 ttttattaga aaaaatagaa actcgaattg tgaaaccaac tattgaaggt ttgcaaaaag
     9541 acggaataga atataaaggt tttgttttta ttggattaat aaacgttaaa gaagaaccta
     9601 tagttattga atacaacgtt agaatgggtg atccggaaac agaagtggta atgcctagaa
     9661 ttaaatcgga tttggtggaa ctttttcaag cagttgctca tcaaaaacta gatgaagtga
     9721 ttttagagat tgatgaaaga agtgcttcaa ctattatggt cgtttctgga ggttatcccc
     9781 aagagtatga aaaaggtttt gaaatatctg ggttagaaaa cattcaagac tctattgctt
     9841 ttcatgctgg aacaaaacta gaaaatggaa aagtagtaac caatggcgga agagttatcg
     9901 ctgttacttc ttatggaagc gactttaaag aagccataaa aaaatcttat caaaatatag
     9961 aaaagctaca ttttgataag atgtattata gaaaagatat tggtttcgac ttattttaag
    10021 aacgaatgtg ccgtagtatc ttggtcttct tcgttattgt cttgatgttt ttttaattgt
    10081 ttacaccaat atacgatgta atatccacat atagccatta aactccaact aataatatta
    10141 gccaaccacc aactgcttaa ttctagatgg cgcaaagcat ctaatggaag aaataaaaaa
    10201 tctacaaata acgattgaat agcttcaaaa aatgctctca tagtataaag tattatattt
    10261 acagtcacaa aagtataaaa tatccttatg ataacaagtc tttttaaaaa atctacacca
    10321 ataaattatt ctttggttgt gcttttgttg attatatttt ttttattaat tcaatttcat
    10381 gctgaaagcc atttgagcag tgcttttagt atgtttaata aaatcagtat attagcaatt
    10441 cttttagcct ctttctttat tgttaacttt attgccaaaa agaatggttt gactaaaaac
    10501 agcagttacc caattttatt ttttctatta ttattatgtt tatttccttc aatttttaaa
    10561 acacctaatt taatttttgc taattttttc ctattattag cctacagaag attggcttct
    10621 ttacaaaatt tgaacgcgat aaagcaaaaa ctttttgacg catcattatg gatttgtgtg
    10681 gcagctttat tccatttttg gtgtattctt ttcctttttc tcgtttatat atccataatt
    10741 tttcatgctt cacgagatta tagaaattgg ttaattcctt ttatagcatt atttactacg
    10801 ttaacaattt tcattttggc agctttagtt tttgatatag cttggattac atatttaatt
    10861 gaaacaacaa aaacgaattt tcaactcgat tattttacca ataatatcca aaatttagcc
    10921 ttatcaatat atgtagtttt atcactctat ttcgtgtttt caatgatctt tacactatcc
    10981 aacaaaccta tgattttaca agcatcttat aagaaaatga tttttggatt ttttattgga
    11041 gtgattgttt ttttaatctc aattaataaa agtaatgcaa tgttggtgtt cactttttta
    11101 ccactagcta ttttatgtac caacaatatt gagtattcac aaaataaaac ctatcaagaa
    11161 atagttttgg gattatttat tttatttggg ttttttagtt tcttttcgca attataattt
    11221 gcttccgtaa gctaaatctc ccgcgtcgcc aagcccagga ataatatagt ttttctcgtt
    11281 tagtctttca tccaaagcag caacccaaag atgacagttt ttaggaagat ttttttgtaa
    11341 ataatcaatt ccttctggag ctgcaattac aacagcaatg tgaatttctt ttggtttttg
    11401 ttctaaatga agtttgttta aaaccgcaac cattgaatgt cctgtcgcca acattgggtc
    11461 aattagaatt aaattttttt cttcaaaact tggtgccgct tgatattcta ccaaaatttc
    11521 aaatttatca tcgttgtcat aatgatggcg ataagcagaa acaaatccgt tttcggcatt
    11581 atcaaaaaag ttcataaaac cagtatgcaa tgccaatcca gcacgaagaa tggagcacaa
    11641 aacaacaggt tcggcaacag ttgtggtgtg ttttatccct aaaggtgttt ggacagttat
    11701 ttctttgtat tctaaagttt tgctcaattc atacgccatt atttctccaa tgcgctcaat
    11761 gttttttcta aaacgcatgc tgtctttttg aatattgatg tctctaattt gtgccaaaaa
    11821 atggtttaaa atactgtttt cttcagaaat ataatgaatg tgcatatcag aaataaattt
    11881 atttgtatcg gtaaaagtat aaaaactatc tttgtacccg agaccatatg gtcgaattgt
    11941 atgaaataaa ataaatttta tagttatgtt ttcagaattt gcctacccaa tatttgaaca
    12001 aagtattaaa gagtatcacg ttattgatga tgtatatcaa cctacaaaaa accctttcga
    12061 aaaaggaagt atagagtatt tgctttatgc aaaaaattgg gttgatactg ttcaatggca
    12121 ttatgaagac atcattcgtg acccaaatat tgatcctgtt gctgctcttg atttgaagag
    12181 aaaaatcgac gcttccaatc aggttcgaac ggatatggtg gaatatattg acagctattt
    12241 tcttcaaaaa cataaagacg ttcaagtaaa acctgacgca aaaatcaata ctgaaagtcc
    12301 agcttgggca attgacagat tatcgatttt agcattgaaa atttaccaca tgaatgaaga
    12361 agccactaga gtagatgctt cgccagaaca tagagtaaaa tgtcaagaaa aactaaacgt
    12421 tttgttagat caaaaaaacg atatgtttgt ttctataaat gatttgttga ccgatattga
    12481 aaatggtgat aaattcatga aagtgtacaa acaaatgaaa atgtacaacg atgatgattt
    12541 gaatccggtg ttgtatctta acaaaaagta atttattttt ttgtcaaaat ctatccaaca
    12601 tatcgctgta attcgtctct cggcaatggg tgatgtggca atgactgttc cagttattag
    12661 agcttttgtg gagcaaaatc ctaatgtaaa aatcacgttt gtttcacggc cattttttaa
    12721 accttttttt gatggaattc ctaacgtttc tttttttgcg gttgatgtaa aaaagcgtca
    12781 caaaggtttt cttggtttac tgaaattgta ttccgattta aaaaagttaa atatagatgc
    12841 tgttgccgat ttgcataatg tattgcgttc ccaaatcatc aggattttgt tttttttatc
    12901 aggaaaaaaa gtagccgcaa ccgataaagg tagagctgaa aaaaaagcac taactcgagc
    12961 cgaaaataaa atctttcaac cagtaaaatc gatggttcaa cgtcatgttg atacctttaa
    13021 tactttaggt ttcaatgttg atttatccaa tccaaaattt ccagaaaaag cggttttatc
    13081 caaagaaata attgaaattt tgggagaaaa acaaaatttt aattggatag gaattgctcc
    13141 ttttgctcaa tatgaaagca aagtatatcc gttagattta atgcaaaaag taattgatga
    13201 attagcattg aatgcttcaa ataaaatttt cctttttggc ggtggaaaca aagaaattga
    13261 acttttaaat caattttcaa aaaacaaaga gaatgttata aatcttgctg gaaaaattcc
    13321 atttcaacac gaattgcaat taatttccaa ccttgatgtc atgctttcta tggatagcgg
    13381 aaatgcacat attgccgcca tgcttggtgt aaaagtcatc acactttggg gtgcaacaca
    13441 tccttttgct ggttttgcgc ctttcaatca accactagaa aattgtttga cttccgatag
    13501 agaacaattt ccactattgc caacatcagt ttatggaaac aaaaaagtag ctggttatga
    13561 agatgtgatg cgaacgattg ctgttgagaa attgattgaa aaaataaatt ccaaccttga
    13621 gtaacaaaat tggaatttag aatttattta ttggaattta attaattaca catcatcata
    13681 atccacataa atctcagatg atgttggatg cgcttgacag gttaaaatca atccagaagc
    13741 gatttcttta tcggttaaaa tagaattttt tgccataacc gcagttcccg atgtgattcg
    13801 agccaaacaa gaactacaaa ttccaccttg acatgagtaa ggcgcatcaa ttccgtgttt
    13861 aagagcagct tccaaaacag tttgtttttg cgacatttca aaagttgttt cttcatcatc
    13921 taccataatg gtaattttag aatgaccttc ggatgaagaa ataaggttat tttcagaaga
    13981 agaagtagaa aacaattcaa atttgatatt tttatcagca atattgtgct cttttaaaac
    14041 atctgaaact cggttaatca tttcttctgg tccacaaagg tagaatttct caaattcttt
    14101 ttctttgtgt ttgttgttca atacaaagtt tacgactgat ttgtcaattc tgccaaaaag
    14161 ttcgccttca acattaactt tactatatac ataatgcaca aaaaaacgcg ctacatattt
    14221 taaatgtaaa tcgtgtaatt gtttgtgaaa aatagtttct tcgggcgttt tatttccata
    14281 aaccaatata aaagtacttt taggttcatt ttctaaaacc gattgaataa ttgacataat
    14341 tggtgtaata ccactgcccg cagcaaaggc cgcatagttt ttttgtcttt caatgctcgg
    14401 ctcaaaagtg aatcttcctt cgggttgacc tacttcaatg acatcaccaa tttttaaatt
    14461 ttcgtttgca aattttgaaa aatgaccatc ttttaccgac ttaatagcta ttcgcaattc
    14521 atcactattg ggtgaagaac aaatggaata cgcacgacgg atttcttctc catcaagcgt
    14581 aagttttaag ttaacatatt gaccagcaat aaattgataa gcaggtttta attcaggcga
    14641 aacagtaaaa gcaacagaga tagcacttgg cgtttcttgt gttacttctt ttatatttaa
    14701 tttataaaat gtggacatga ggtttatttt tcgcaaagat aataaacagc aaagaggaaa
    14761 cgaaaataaa aatataccta agaatgtaat gtatgttatt ttcttatgta tatttgtagt
    14821 tcaaaatgat gattaaatga agaattcaca attgtataaa ggaagtttaa acaccatagt
    14881 gatgaagctt ttggaagaaa acggcagaat gtatggttat gaaattaccc aaaaagtaaa
    14941 agacattacc aaaggcgaac ttaaaataac tgaaggcgcg ttgtatccgg cattgcacaa
    15001 aatggaagcc gaaggaattc tcgaagttga ggtagaaaac gttgacaatc gcttgcgaaa
    15061 atattacaaa ctaactcaaa aaggaacaac cgaaacagtt aataaactag ctgaactaga
    15121 agaatttatc aaaaacatgc aatctattgt acaacctaaa ttaggatatt aaatatgaaa
    15181 ctttctaccg aacaaatcga gaaattatac caattcacac gccaacatta tgtggaatat
    15241 tatgatttac aaaccgaatt ggtcgatcat ttagccaatg gaattgaaga acaatggcaa
    15301 gaaaaaccaa aactttcatt tgacgaagca ttgcaagtag aattcaaaaa atttggtgtt
    15361 tttgggttta tggatgtagt agagaaaaga actgctgcga tgaataagcg ttataataaa
    15421 atgattttaa accatttgaa agtgtatttc agaatcccgc aaatcattgg aaccattgct
    15481 gcagttggta tagtgtttta tttgctaaag atgagtttgg tttctgtatt tacgatgtca
    15541 atattgggtt taataagtat tggattttta tttttttcac tattttattt agtacgaaaa
    15601 agaaaaaaat atgaggcaaa aactggaaaa aaatggatgc tgaaagaaat tatttttgga
    15661 cattctactt ccttgggatt aattcagttt cccatacagt tatttttaca aatcgatgag
    15721 ccgttagtgg atatttggct tattattttc agcgtgtttt ttgtactgat ttttatttgc
    15781 gagtatatta ttatttttga aataccatca aaagccgaag attatttaaa gcaaacttat
    15841 ccagaatatg aattttctga aagctaaaat gtaacatttt ttacatatca aatacttact
    15901 agcaaaccta aaccactcaa ctatgataaa acatttcatt tctctcgaat ggaaatcctt
    15961 ccttcgttcg gcttcttttg gaactaattt agcactgaaa atatttatgg cacttggtgc
    16021 tttgtatttt gcagccatgt ttgttggact aggtattgct gtttattttc tcattgaaga
    16081 tgcaggttta aaaccatttg aaacggtcaa taaatttcta atttactatt tcacagccga
    16141 tttagttatt cgttattttt tacaaacaac accagtttta aacatcaaac cattaacaat
    16201 tttaaacatt aagaaaaaca ccattgttaa ttttgtaatt ggaaaatctg ctttatcttt
    16261 ttttaatttt ttacatgcgt ttttctttat accgttttca atagtattag taaaagaagg
    16321 ttttaaccca atgcaagtag ttttttggca tattggtgtt ttcagtatta tttattctat
    16381 gaatttcatc aatgttttag tcaacaaaaa agatagtatt ttctatgttg ttttgacaat
    16441 cgttggtgga tttggattag ctcaatacta tggttggttt gatgtaacgg tttatacttc
    16501 tccatttttt caaggaatgt atgataccag ttatttgttt ttatttccaa tcgcagcatt
    16561 agttttatta tattatttca acttcaaatt tttcaaaaat aatctaaccc ttgatactgg
    16621 tttagcagta aaaagtaaag aagccacaac tgaaaacctt acttttttag accaatttgg
    16681 aactttagga acgtttctta aaaacgacat caaactttta aaacgcaaca aacgatctag
    16741 aacaactgta attatgagcg ttgtttttat cttttatgga cttttgtttt ttacaaattc
    16801 tatagaagct tataacaatc ctgccatgca tgtttttgct ggaatatttg tttcaggtgg
    16861 atttcttttc acgtttggac aatttgtacc aagttgggac agcgcttatt atcaactaat
    16921 gatgagtcaa aacattccat accgcgaata catcaaatca aaatggtggt tgatggttat
    16981 tggaacggtt gtatctacca ttttggcatc attttattta tattttggtt ggcatattta
    17041 tttattaatt gttgttggcg ctatttttaa tataggtgtc aattcgcatt tggtaatgct
    17101 tggcggtgcc tttgtaaaaa caccaattga tttgtctagt tctaaacaag cctttggaaa
    17161 caaacaagcc tttaacatga aaaccatgct gatttctttg ccaaaattat tattaccaat
    17221 ggctttgtat gcagcgggtt attatatttt ttcgcccgaa gttggtttac tttttgtggc
    17281 tggagcagga gttttaggtt ttgcttttag aaacaaagta tttaccatga ttgaaaaaat
    17341 ctataaaacc gaaaaatacg ctactatttc agcatacaaa caaacaaact aaggactatt
    17401 cactaacaac taagtactaa aaacatgata caagtaaaca atctttcaaa aatatatacc
    17461 aacggtaaaa aagtattaaa cattgccaat cttgaaattc caaaaggtca aagttttggt
    17521 ttagtaggaa ataatggtgc tggaaaaact acttttttta gtctattatt agatttgatt
    17581 gagccaacaa caggttcaat aaaaaacaat gaagtaattg taaatcaaag cgaagcttgg
    17641 aaacctttca cggctgcttt tatagacgaa agttttttaa ttggttattt aactgccgaa
    17701 gaatactttt attttatagg tgatttaaga ggtcaaaaca aagccgatgt tgatgcatta
    17761 cttaaaaagc acgaacaatt tttcaataac gaaatcctta acaacaaaaa ataccttcgc
    17821 gatttatcga aaggaaacat gaagaaagtc ggaattattg cggctttaat cggaaatcca
    17881 gaagtagtaa ttttagacga accgtttgcc aatcttgacc caacaaccgt tagccgattg
    17941 aaaaaaatca ttaaagagct agccgataat cctgaggtta ctgttttagt ttccagtcac
    18001 gatttagtgc ataccgttga ggtttgcgat agaattgtag cattaaatct tggcgaagtg
    18061 gtaaaagaca ttcaaacttc gccagaaacc ttgaaagaat tggaaacttt ttttgctgtt
    18121 taacgacgaa tatataaaat tagaatagct gtattgtcca aaaaacatgc agctattttt
    18181 ttgctatatc aaaaagaaac gtatttttac cagtcagtta tactaaaaag tatattttta
    18241 cgttataaat caaacgtttt acattgaaaa ctaaaattac aaagcgcatt cttttcttcg
    18301 gatttctatt tttcttgata gcttgttcta ccaaaaaaaa ttcatttctg tctagaaact
    18361 cacatgcatt aagctcaaag tttaatattt tatacaacgg tgatcttgcc ttagaacaag
    18421 gattgctaga gttaaaatct caatatcaag ataatttttg ggaaattctt cccattgaaa
    18481 gaatgcaaat aaaggaagaa caattgcttc caaatgagac taaaaaccca aactttgaac
    18541 gtgccgaaga aaaagctacc aaagccattc aaaagcattc tatgaacatt caaggttcgg
    18601 aaaaaaaccc gcaaatggat gaagcgcatt tacttttggg aaaagccagg tattatgacc
    18661 aacgatttgt tccagctttg gaagccttca attatgtatt gtacaaatat ccaaaaagca
    18721 gtaaaattta tgaagtaaag atttggcgtg aaaaaacgaa tatgcgattg gaaaacgacc
    18781 aactagcagt taataatctt agaaagttgc taaaagaaat taagtttaaa gatcaaattt
    18841 ttgccgatgc caacgctgtt ttagcacaag cttttttaaa tttagaagta aaagacagcg
    18901 cagttgctaa actaaaatta gctagagagt ttacaaaatc aaacgaagaa aaatcacgat
    18961 atgattttat tttgggacaa ttgtatgaaa atttaggaca caaagacagt gcttatgcaa
    19021 gttaccaaaa agtaattgat atgcatagaa aagctgcaaa acaatataca attcatgctc
    19081 atattaatca agtcttgcag caagagtata aaaaaggaga tacgttgctt tttctaaaaa
    19141 aatggaacaa gctacttgcc gacagagaaa acaggcctta cttagatgca ttgtaccatc
    19201 aaaaagcaaa gttttatgaa ggacaagaaa attttgaagc cgcaaaaaag aattacaatc
    19261 tatcattaaa aaagaaatcg caagaccaat atttgatggc ttctaactat agaaatctgg
    19321 caaccattta ttttaatgat gcaaaatatg ctacagctgg aaagtattat gatagtactt
    19381 tggtgaattt aaaaccaaga acaagagagt ttaatgctat aaaaaagaaa agagaaaatc
    19441 tagaagatgt tattaaatat gaggctattg cacaaaaaaa tgacagtatc atttcattgt
    19501 acaaaatgtc ttcttctgat aaagtagctt attttgacaa atacatcgaa aaacttaaaa
    19561 aggaagatgc caaaaaactt aaagaattag aaaaacaaga acagattaaa gtaaattcag
    19621 aatcttttgg tggtgacgaa tctcaatcta tcaatgcagc aaaaatggct gcaaatgttc
    19681 caaaaccaaa tctttttacg ggttccaaaa ccgattttta tttctataat ccaactacaa
    19741 ttgcttacgg aaaaaaagaa ttcacaaaaa tttggggaaa aagaaaattg gtcgataact
    19801 ggagagtttc atccttgtta accaaagaag acgccaaagg aaatgatgaa aaagatgcca
    19861 aagaaaatgc cgtaacctca aaagaaaaag acggaaaaga aaattcagat gaaaatccag
    19921 cctatacttc cgatttttac attagtcagt taccaacttc acaaaaagta attgatagtt
    19981 tatcaaaaga acgaaatttt gcctattatc aattaggtgt aatttataaa gaaaagttca
    20041 aagaatacca actggcagtc aataaattgg agcaattgtt aaaaaacaat cccgaggaac
    20101 gattgatttt gccttcgatg tacaatttat ataagcttta tgaaatttta gataaaaata
    20161 aagcattggc aatgaaagct cgaattattg cggaatatcc ggagtcaaga tatgctcaaa
    20221 ttcttagcaa tccacaagac caattggcag atgataataa ttcaccaaaa gcagtttatg
    20281 aagcattgtt taaacaatat caagaacgaa attttaaaga ggttttagta aaaatagacg
    20341 aagctattga gcgatttaca ggtgatgaaa ttgcaccaaa attagaatta cttaaggcta
    20401 atttaattgg taaactaaaa ggattagatg aatttcagca agcattaaat tatgttgcat
    20461 taaattatcc aaatgtagaa gaaggcaaaa cagctgagaa aacaataagc attagtttgc
    20521 ctaaattacg agctttgcaa cttggaaaat atgaatcgac aaattggaaa atcttatacc
    20581 aatcaaaaga ttttgatgat aaaaatacta aagcacttcg cgaaaaaatc actaaattta
    20641 ttaaagaaag aagtttagat aagctaacca tttcgttaga tgtttataca atgaccgata
    20701 attttgtggt gatacacgga atgaaatcgg aagactatgc taaaggaata gcttcaattt
    20761 taaaagaatt caaagaatat aaagttcaag acacaccgat tatcatttca acagagaatt
    20821 attcagtagt tcaaattaag aaaaatcttc aggattatat agacggtaaa ttagttgaca
    20881 atcccaaaga accaaattgg gatggaagta tagaaaaagc tcctgtggca caacaggaac
    20941 ctccaaaaca agaggaaaaa ccaagaaata ccaaagccga acaaaaagca attcagaatc
    21001 aaaaagaaga gttaaaaggt gctcaatcga tacaaaatga taaaaagata aaggcaacac
    21061 aaaatgaaga aaatactttt ggaccgccac cagcaccaca aatgccgaat ggaaaataat
    21121 taaaattatg ttcgaaaaaa gatcaaagcc ttacaccgat ttattaggaa aaacaaatag
    21181 aattgtagaa ggaacaacaa ttaatggaaa tgttgtttcg gctacagaca tccgtttgga
    21241 tggacaactt ataggcaatc ttcaatcaaa aggaaagtta gtaattggtc caacaggtaa
    21301 tattaaagga gatgttattt gtagaaacgc agacatagaa ggacttttcg aaggtaagat
    21361 tcaagtgcaa gaaattttaa atataaaatc aaaagcacat ataaaaggtg atgtaatttg
    21421 cggtaaatta tccgttgaac caggagcaga atttagcgcc acttgtgcca tgaaaccaca
    21481 agtaaaaaac ataacgactg atggaaaatc agaacaagaa aaaatcgcct aataaatggt
    21541 tggttctaat taatattccg attcaaatgg gaattgtaat attccttttt gcctatttag
    21601 ggaaatatct agacacaacc tatccaaaca cacattcagt ttatgtaaaa gtactaacac
    21661 tttcaggtgt agctattgcc ttttataatt taaaccgaca attaaaaaaa ctcaacgatt
    21721 ctgataaaca atgaatttca aaactcaaaa accagtcatt ttagcaatag ttttttatac
    21781 cattcataaa gtaatttttt tagttgcttt tccaaaagaa gaaattaata cgtttgtcta
    21841 tccattagaa ttgttgtatt tgttttttgg atttagttct gttttaattg tttttttatt
    21901 agtaaaaatt aatcaaaaga acattaataa tgttggttat acatttttat tagtcacttc
    21961 tgtaaaaatg gcattagcct atttttttct tcagccgatt ttaaattcta aaaccgaatt
    22021 tatcaaaatg gacaaaatga atttttttgt catatttatt gtttttttga taatagaaac
    22081 cagcgtaact ataaaaatgt tgaacaaaaa acaataaaag ctttaaaaag tgataatttg
    22141 ataaaaaaat caaatcgaat acttttaaat actaatttat attgtacttt tgcaccaaat
    22201 ttgagaaaac aaaatttaag ataattttca gatatggtga tttcaaaaaa accacgcctt
    22261 tttatatatg cagctttagt tgcacttttg ccgttattcg gtttagccaa tccaaacgat
    22321 tctactacag tttctcatca agttgctcac gcagacaaaa ctactcatga gaatacagaa
    22381 ggattaacca aggagcagct aaaagtaaaa gaaacaaaag aattcatcag acaccacttg
    22441 ttggattcac atgattttca tttgttttct tatgggaaag atgaggctgg acatgaaaac
    22501 catgtaagtt ttccacttcc agtaattctt tgggacaatg gaattcattt ctttatgtct
    22561 tcaaaattcg aacatggtca tgcagctgct gagtcaaaag ggaattatta tgctattaat
    22621 catcacaacg gacaaattta taaaacagac gcagcaggta cttttattga aaacgataaa
    22681 ggtaaaatag caaatgacaa accaattgat ttatcgataa caaaaaatgt ttcgatgata
    22741 ttaatcacag gtttaatcat gttcttatta tttgcaggtt taggaaaatc atacaaaaag
    22801 aatggttcaa ttgctgctgg agctggaaga ttttttgaac ctattatact ctatattaga
    22861 gatgaaattg ctattcctaa tattggagta aaatattata gaaaatacat gagtttcttg
    22921 ttaacaatat ttttctttgt ttggttctta aacatgtttg gtttaacacc gcttggagta
    22981 aacgtaacag gaaacatcgc aatcacagcc gcattagcta ttattgctta catcatcaca
    23041 acatttaccg ctaaaaaaga ttattggaaa cacattttct ggatgccagg cgtacctgtt
    23101 ccaatgaaat ttattttagc accaatcgaa ttgttaggaa catttatcaa accattttcg
    23161 ttaatgatac gtttgtatgc taatatgtta gcaggtcacg tggtattaat gagtttgatt
    23221 gcattaatgt attctgcaaa tcactttatt ggaagtccat tagcattatt attgtctttt
    23281 gtactaacaa tattagaagt tttagtagcc ttgttacaag cttatatttt tacaatgttg
    23341 gctgctttat attttggttc ggctgttgaa gaacaccacc atgaagaagc acattagtat
    23401 tttgacatta gatttctgat tgtagatatg tcagaaatat aatatctaaa atctaatatt
    23461 aaaaagaatg tttaattaaa tatatataca ttatggaaat tcctagaatt attggagcag
    23521 gattagtagt aatcggagct ggaattggta ttggtagaat cggtggttct gcaatggatg
    23581 ctattgcacg tcaaccagaa gctactggaa aaatccaaac agcgatgctt attgctgctg
    23641 cacttattga aggtattggt ttcgctgcaa tttttgcaac gtaattaaaa atttttaaca
    23701 aaagctataa cggttggttg tagcttttgt tactttatta aaattaaaaa gatttaacac
    23761 ttatataaaa tggaagaatt atttggacag ttttccttag ggttgtttat actacaattc
    23821 gttttattcg ttgcattagt ttttcttttg aaaaaatttg catggaaacc aattcttgac
    23881 gctgtaaatg aaagagaaga tggcatcaaa aatgcattgc tttctgcaga aaatgcaaaa
    23941 aaagaaatgc aaaatttgaa atctgataat gagaaattat tagcagatgc aagattagag
    24001 cgtgacgcca tgatgaaaga tgctcgtgaa atcaaagaaa aaatgattaa cgatgcaaaa
    24061 acagaagcgc aagtagctgg agaaaaaatg attgcacaag caaaagccgc tattgaaagc
    24121 gaaaaaaatg cagcaatggc agaattgaaa gcacaagtat ctaatttatc tttagatatt
    24181 gcagaaaaag tattaaaaag cgaattgtct aacaaagaat ctcaaacgaa attagttgag
    24241 aaaatgttag gtgacgttaa attaaattaa tcgatatgtc tagagctgcg attagatatg
    24301 caaaagcgat tttagaaata gcttctacat caggaaacac tgctaaagtg aataatgaca
    24361 tgaaagctat tactgaagta gtaagcggaa ataaagaatt aagcgatttt ttggcaagtc
    24421 caatcattac ttcagatata aagatgaatg ctttgtctga agtttttgga actgtagaaa
    24481 atgagacaaa atcattattc cgtttattgc aagaaaacaa aagatttgag atattgttac
    24541 caatagccga acaattcaat gcacaatatg atgttttgaa cggagtagaa gtagcagtag
    24601 tgactacagc aattccgatg gatgctgctt tagaaacaca agttttagct aaaattgcaa
    24661 caatttcaga taaaaaaatc actttacaaa acacaattga tccttctata ataggtggat
    24721 ttattttgcg aatgggtgac aaacaatata acgcatctgt agcaaacaga ttgcaagaat
    24781 taagaagaga gtttagtaat taaattacca ttaaagatat atcaaaatgg cggaaattaa
    24841 agcagctgaa atatcagcaa tattaaaaaa acaggtagaa ggatttcaat ctggtgctac
    24901 tttagaagaa gttggatcag ttttacaagt aggtgatggt attgctcgtg tttacgggtt
    24961 gtccaatgct caatacggag agttagtaca attcgaaaat ggattggaag ctatcgtttt
    25021 gaacttagaa gaagacaacg ttggtgttgt acttttagga ccatctacag gaattaaaga
    25081 aggctcaacc gttaaaagaa cgcaacgtat tgcttcttta aatgttggtg aaaacatcgt
    25141 aggtcgtgtt gtaaacacac ttggagaacc aattgacggt aaaggaccaa tcggtggtga
    25201 tttatacgaa atgccattag aaagaaaagc acctggagtt atcttccgtc agccggtaac
    25261 tgagccatta caaacaggaa ttaaatcaat tgatgcaatg attccagtag gtcgtggtca
    25321 acgtgagtta gttattggtg accgtcaaac aggaaaaact acagtatgta ttgacactat
    25381 cttaaaccaa aaagaatttt atgacgcagg tcaacctgta tattgtattt atgttgctat
    25441 tgggcaaaaa gcttcaacag tagcaggaat tgcaaaaacg ttggaagaaa aaggagcaat
    25501 ggcttatact acaattgtag cagctaacgc atcagatcca gcagcgatgc aggtttatgc
    25561 gccaatggcc ggagcagcaa ttggagaata tttccgtgac tcaggtcgtc cagcattaat
    25621 catttatgat gatttatcta aacaagcggt tgcttaccgt gaagtttcat tattacttcg
    25681 tcgtcctcca ggtcgtgaag catatcctgg tgacgttttc tatcttcact ctcgtttatt
    25741 agaaagagct tgtaaagtga ttgctgatga cgatattgca agaaacatga atgatttacc
    25801 agattcatta aaaccaatcg tgaaaggtgg tggttcttta acagcattac caattattga
    25861 aacgcaagct ggtgacgttt ctgcttatat tccaacaaac gtaatttcga ttactgatgg
    25921 acaaattttc ttggatggag atttatttaa ctctggagtt cgtcctgcta ttaacgtagg
    25981 tatttcggta tctcgtgttg gtggaaatgc tcagattaaa tcaatgaaaa aagtatcggg
    26041 aacattaaaa cttgaccaag ctcaattccg tgaattggaa gcgtttgcta aatttggttc
    26101 tgaccttgat gccgttactt taaacgtaat tgaaaaaggt agaagaaacg ttgaaatctt
    26161 aaaacaagcc gtaaacgatc cttatactgt agaagatcaa gtagctatta tttatgcagg
    26221 ttcaaaaaat ttattaaaaa ccgttcctgt aaataaaatt aaagaattcg aaaaagatta
    26281 tatcgaatat ctaaatgcaa aacatagaga tactttaaac gctctaaaag caggtaagtt
    26341 agacgacaac attactgatg ttttagaaaa agcggcaaaa gaaatctcag cgaaatataa
    26401 ttaaaaagta ttaagtatta agtatcaaga attaagacat cttgatactt aatactttta
    26461 tcttaataca agaaaaatgg caaacttaaa ggaaatccgt aatagaatta gttccgtctc
    26521 gtcaacgatg cagattacat cggcgatgaa aatggtttct gctgcaaagt tgaaaaaagc
    26581 acaagatgct attacagcaa tgcgacctta tgccgaaaaa ttaacggaat tattgcaaaa
    26641 cgttagcgca acactcgacg gtgatgctgg tggagaattt acttctcaac gcgaaatcaa
    26701 caaagtgttg gtggtagcaa taacttcaaa cagaggtttg tgtggtgctt tcaataccaa
    26761 tgtaattaag caagcgaaac ttgttgccga aagttacaca ggcaagcagg tagatatttt
    26821 tgctattggt aaaaaaggaa acgacatttt aagaaaaaca tataacgttg tagataataa
    26881 aagtgaagtt tttgatcaat taagttttga taacgtagca gatattgcag acgctttaac
    26941 cgaaaagttt acttcgggcg aatacgacaa aattgaaatc atttacaatc agtttaaaaa
    27001 cgcagcaaca caaatcgttc aaaccgaaca atttttaccg ctagcaccaa ttgtttctga
    27061 tgttccggtt tctactggcg attacatttt tgaaccatca aaagaggaaa tcgtgttgac
    27121 tttgattcca aaatcattga aaacacagct atacaaagcc atcagagatt catttgcatc
    27181 cgaacacggc gcaagaatga ccgcaatgca caaagcaacc gataatgcaa ccgaattgag
    27241 aaatcaattg aaattaacct acaacaaagc acgtcaagct gcaattacta acgaaatcct
    27301 tgagattgtt ggtggtgcag aagcgttaaa aggctaataa aaaatatatt tttataaaaa
    27361 gagtcaacat tcgttggctc tttttttatt cgttcaattt aatagttatt tttgtttcat
    27421 aaagtttaaa tattcacctt gagcatctat aaaaatcttt tcaaacaaac catgatttat
    27481 ggcttagcta cggttttgcc aaggatgttt agttttattt tagtcccgct ttataccgat
    27541 ttactaccaa aagcagagta tggtcaaatc accgtaatct ttgcttacat gattttcttt
    27601 aatgttatct tggcttacgg aatggaaacc tcgttcttcc gattttacaa caaagaagac
    27661 gacaaaaaga aagtaatcga aacctcgatg gtcactattt tctggacgac cattctgttt
    27721 ttagcaggcg cactaacctt tagaaacgac attgcaaagt tcatcgaaat tgatgtccaa
    27781 tacgtcacct attccatttg gatactggtt ttagacgcgc tagttatcat tccgttttca
    27841 aaactaagag ccattcagcg accaagtttc tatgcgtttc taaaaatatc gaatgtagtc
    27901 gtaaaccttt cgcttaatgt tttcttcctc gtatatttac ccaaaattgt agccagtcaa
    27961 cccagcggtt tcttaagttc tatctatttc gaaaacttcc aaattggtta tgttttcatc
    28021 tcaaatgtaa ttgccagttt gctcacattc gtcttgcttt ccaaagatta ttttacctta
    28081 aattggaagt tcgacgtgca actttggaaa caaatgatga agtatggaat gcccatcttg
    28141 gtttccggag ttgcttttgc catcaacgag cagttcgaca aaatattatt gcaaaaactc
    28201 ttgccgccca atattgccga agcagaagtt ggcgtctatt cggcttgtta taagctcgga
    28261 ttattcatgg tgttgttccg aacggcctat actttgggta tcgaaccgtt tttctttagc
    28321 catgcaaaaa acgaaaacgc gccacaaacc tacgccacca tcaccaaata ttttgtcatc
    28381 tttggttcct tcatcatgct aaacgttatc gtttttgccg accttttcaa gcagttaatg
    28441 attcgagatg cttcttattg ggaagcgatg aaagttgttc cgttaatcat tttggccaat
    28501 ttcttcttgg gaatttacac caacctttcc gtttggtaca aactcatcga caaaacctat
    28561 attggcgcac taatttcgct ggttggtgcc gccgtaacct tggttttaaa cttctggtta
    28621 atcccaaaaa cgggttattt tggtggttat tatgggtctg ccattgctac aattgcagct
    28681 tatgggtcga tgatgttact ttcctattat ttgggcaaaa aacagtatcc aattccttat
    28741 gaaatgaaga aaatcggcct atatttgatg ctttcggttg ccttttccat cgtatctttc
    28801 tactttttta gagaaaatta tctagtgggc attccattaa ttttgatttt cttatatttc
    28861 atctattaca acgaaaaaga aactttagcc acaatagtaa aacgcaaaaa atgaccataa
    28921 aaatcatcaa caaatcgcaa cacgaacttc caaattacga aaccattgct tctgcaggaa
    28981 tggatttgcg tgccaattta gaagcaccca ttaccctaaa atcattagag cgaaccattg
    29041 taaaaacagg tttgtttatc gaattgccca ttggttttga agcccaagtg cgcccaagaa
    29101 gcggtttggc agccaaaaaa ggaatcaccg ttctaaatgc tcccggcacc gtggatgctg
    29161 attatcgtgg cgaaattggc gtgatattgg tcaacctatc ttccgaagat ttcaccatag
    29221 aaaacggcga aagaatagcc caattaatca tcgccaagca cgaacgcgcc gaatggatgg
    29281 cagttgaaac gctatcggaa acctctagag gcgaaggcgg ctttggaagc actggcgtga
    29341 agtaattttt ctcaaaaaaa cacgcttttt ccaaaacaag tttccaagta gcaagataaa
    29401 cttgcatccc ttcagaactt ttaaagtcat ccgcaagata gaattgcatc gctttagacg
    29461 ttttcatagt aaacacaaga tagaattgca tctttatagc tattttgaca gtgtatgaaa
    29521 gataaaattg catcccttta gacagtttct tagtaaatgc agactatttt tagacggaaa
    29581 aagatttttt ctatacaaaa acaatctttt attatataaa aaattaattt tttgactact
    29641 ttttcattcc aaattattat ttttgaaata gttttagtag cgaatattgt gaaaaattat
    29701 tcttttaaat caaaatacta tggcaacagg atttcactta ggcaattatt taacaaaact
    29761 catcgataca aaacgcttgc gtagaaaaga aattacgcaa cagatgggcg tacaatattc
    29821 tgccttctat ggctacgaag gccgaccgtc gttgcagttt tatattgtgc tgcgtctttg
    29881 tcatgccatg aaatacaata tttttttgga tgtagccaac atgttgcctc ccgagtatga
    29941 ctatgataag aagagcagca ccggaaaaga caacatcatt gcgcaacttg aggaagaaaa
    30001 taaaaagttg aaatgggaaa acgatttgct caaagaaatg atgattaaaa aaggatagaa
    30061 attcggtaaa ttggcaacaa ttgctcgggt ttttacccta tttttgtgaa gatataatca
    30121 aaaatcaata gatagatgaa aattattgta cccatggctg gacgaggttc cagattacga
    30181 ccacacacac tcaccattcc aaaaccgtta attcctattg ccggaaaacc aatcgtacac
    30241 cgcttggtag aagacattgc gggtgtatta aaccaagata ttgaagaagt agcattcatt
    30301 attcacgaaa gctttggcaa aaaagtagaa gacgaattga ttgcgatagc cgaaaaacta
    30361 ggcgccaaag gaacgattta ctatcaaaac gaagcactcg gaacggggca tgccatcatg
    30421 tgtgccaagg attctttgac tggttcggcg gttattgctt atgccgatac gttaattcgt
    30481 gccaaatttg atttagacca aaccgccgac agcgttattt gggtaaaaca agtggagcaa
    30541 cccgaagctt ttggcgttgt aaatttgaac gacaacaacg aaatcatcga attggttgaa
    30601 aaaccacaag aatttgtttc ggatttggcg gtcatcggga tttattattt caaagacgtt
    30661 gcggttttga aaaacgaatt gcaatcggtg ttggacaaca atatcattca cggtggcgaa
    30721 tatcaaatta acgatggtat caagcaaatg atggcaaaag gcatgaaatt tgtacctgga
    30781 gaagttgacg aatggatgga ttgtggcaat aaagacgtga ctgtggaaac caattcgcga
    30841 atgttagggt ttcttcttaa cgatggcgaa aaattaataa gcgatgtggt tcgtgttgaa
    30901 aattcgaccg tcattgagcc ttgttttatt gatgatgatg tggttttaat caactctatt
    30961 gttggtccga acgtgtcgct tggaaaagga acgcatctta cgaatgtgaa gattcaaaac
    31021 agcttggtgc aaacccatgc gcacattaga aacgctaaat tggacaacgc gatgatagga
    31081 aatcacgcta catttgatgg cgaatttacc agcattagca tcggcgatta ctcggtttta
    31141 gagtaactat cctaaaaagg gttaaccgat taggcagaaa atatagatat gaaaaaaata
    31201 gttttacatc gttttctttt tgggatattg tttattccaa ccatcctaat ggcgcaaacc
    31261 gagcctgagg atgttgcctc gattaccaat gaatttgaga ataacttctt tgaatcattg
    31321 aaacaaaagg gtattgagaa ttatgacaag gctatcgagt cattggataa gtgcttggtg
    31381 cttgaacccg ataatcccgc agttttattt gagttgggca agaattattt ggcccaaaaa
    31441 aagtatccca ctgcctattc ctattttgaa aaggcaactc aaattgatgc caaaaaccgt
    31501 tgggcttggg taggcatgta tgatgtttgc catgaaacgc aagattataa caaagccatt
    31561 ccaatcgttg aaaaattgat aacatttaaa gacagttata aggaagattt ggtgtcgctt
    31621 tatatgaata cgcagcaatt tgacaaagcg ttggagttga ttaacaattt gaatgaaacg
    31681 gttggaaaat cggagaagcg agaattgtat aaagccgata ttcttagaga tgccaagtac
    31741 caaagtgccg agaaattcaa tttgttagac caaatcaaga agtttccaaa ggaagagtcg
    31801 aattatattg cgttgatata tatgtattcg caaagcaatc aggaagaaaa agcgttggaa
    31861 attgccgaaa aattagagaa agagattcca acttccgatt gggcgcaagt gagtttgttt
    31921 aaatttcatt tgaataacaa tgatggagca caagcggtaa aagcgatgaa tatagtattg
    31981 ccaagcgcaa agattgattc gaaaattaaa caccgaatgt tgaatgagtt tttgatattt
    32041 gccaccaaca acccacaatt tgaacctgat ttagacaaag cgattgccta ttttgataac
    32101 gataagaatg ttgacgttgc taaagaaata gggaagtttt atttcatgaa aagcgattgg
    32161 gcaaaggcaa aaaagtattt tgagttgcaa acaaaaaccc attcggatga catagaatca
    32221 caaaaattat tgttgcaatc gtatgttgaa ttgcaagatt ttaaagcagt agccaaaaaa
    32281 gcagatgact taacacagta ttatccaaca caacccgatt tgtattacta tgctggcttg
    32341 gcgcaaaatc aattgggcaa ttttaagaaa gcaaaagagg cactcgaagc cggacttgac
    32401 tttatcattg atgatgtttc attagaaatt aatttctact tgcaattaag tgaaactttt
    32461 aatggtttgg acgatgtgaa aaagaaagaa agttactttg ccaaagcaaa tgatttatca
    32521 aaaaaacaaa agaaatgaaa aaaataatat ttgttgtttc aatatgtttt tttgcgatgt
    32581 cctgtaagtc caaaaaagca cttatcagcg aaggaattgc cacggaaaag ataaaagcga
    32641 cagaaataat taaaaatcat tacaacaata aaaatgattt taaaacgtta tatattaaag
    32701 cttcggcacg ctatgaagat ccgaaaaatg caaatagtgt tacggctgaa gttagaatac
    32761 aacgaaatga aaagatatta gtaagcattc gttttttagg tattacaatg gcaaaagcat
    32821 tgattacgcc aagtaatgtt tactattacg aaaaaattaa cggcacttat tttgaaggtg
    32881 attatgagtt actaagccaa ttcttaggca ccgaattaga ttatacaaaa gttgaaaatt
    32941 tgttgatagg agaagccatt gacgatttga ataaagaaaa atttgttgct ttaattgtag
    33001 atagttttta caaattgaat aacgtaaaca caaatgaaat tgacaaaaca ttctattttg
    33061 aagccgataa ttttttagta aagaaagaag aaatcattca aaacagtaaa aacagaaaag
    33121 taacgattga gtatccaaat tttcaaaaag taggagaata tatgctgcct gctgccatag
    33181 aaatcaatgc agaacaggaa aaagggaaaa cagcaataag tataaaatac aacaccatta
    33241 atttgaacga cgaactttct tttccctatt cggttccgga tggttatgaa agaatattta
    33301 ttaagtaaat ttgcaaaaaa accaatatga caaagcatat tttaacattt ttattctttt
    33361 gtttagcact gccaatgtgg agtcaacttt ctaaacaaga gcagttggaa gaacgcaagg
    33421 ccaaaattca attggaaatc caagaacgtc agcagctgtt gaactctatg aaaagcaaag
    33481 agaaaactgt ggtaagtcag ttgatgattc aaaaagacaa gataaattta aaagaacagt
    33541 tgattaaaac taccgaaaag caaaccaagc ttttgaacga tgatatttat agaaatcagc
    33601 ttaaaataaa tcaattaaac aaagagttag aggttttaag agaagattat gccgagatga
    33661 ttcgcaagtc ctataaaagc cgttccgagc aaagtagagt aatgtttttg ttgtcatcgg
    33721 aaaattttct gcaagcttat aaacgcattc aatacatgaa gcagtacaac aattacagaa
    33781 aaatgcaagc agatgaattg aaggcaaaaa ccacaaaatt aaagtcaacc actgaggaaa
    33841 ttaaagttca aaaaacggag aaagaaaaac taattgttga aaatgaaaag gaaaagcaag
    33901 agttagaaaa agaaaagcaa gagcaagaaa aaattgccaa acaaatacaa aaagacaaga
    33961 aaaacattgt tgctgaaata aaaaagaaac agcaagaaac caagaaaata gatgctcaga
    34021 ttcaaaaatt gattcgtgat gctattgccg aagcgaatag aaaaacggca aaagcaacat
    34081 cctctaaaac aaaaacaacc gcagaaaaga aagccatcga gaactcggta aatattgttt
    34141 taacaccaga aggaaaatta atttcggata attttaaagc gaacaaagga agattgcctt
    34201 ggccagtaga aaaaggtgcc attacgcttg gttatggcga gcaaccacat cctgttttta
    34261 gaacattaac ggtctataat agcggaattg aaattgctac agagaatggt gcagatgcaa
    34321 aagccgtatt tgatggcgaa gtttctcaag tgcaattaac atcgccacaa aagaaaactg
    34381 ttattattaa acatggagat tttttcacag tttataacaa tttatcaacc gtaaacgttc
    34441 gagaaggcga taaagtttca gcaagagaaa ctatcggaaa aattagaaca gatggtgatg
    34501 ggaaaacgat tttaaagttt atgatttcac aaaacactac ttattttaac ccaacacctt
    34561 ggttgtctaa ataaggttaa cttttaaaac acaaaaaacc ctttcagcgt attaaattct
    34621 gaaagggttt
//
LOCUS       DAOOBH010000012        31773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_1835_length_31773_cov_5.015922, whole genome shotgun sequence.
ACCESSION   DAOOBH010000012 DAOOBH010000000
VERSION     DAOOBH010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 31773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31773
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_31773_cov_5.015922"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PK218_02855"
     CDS             complement(<1..887)
                     /locus_tag="PK218_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97479.1"
                     /translation="MKRKLLFKKGLHLTFWCFSLVFLLLPSNSVGQAFTGTYTFTGSS
                     GNVSSFAYNGSTITNLTVSPLTKEGVTTSSSSGNFRANDWDTGSTDGGAAGGSVNLSK
                     YFQFTISATSGYTLSNMSINFGAGRSSTGPRLFQWRSSVDNYATLLPLTSSNGSVSIT
                     SNVLQIPDANNGYTGNTATSSATGVTSITFRVYAYGSESTGGTGGLQGNLIFNGTLAA
                     VVTAPVVTTDLTPSSIGTTSATLGGNVTSDGGSAVTGRGIVYAASATNTNPLIGGTGV
                     VDSSTTGTTGTFSVSESSL"
     gene            complement(1141..3930)
                     /locus_tag="PK218_02860"
     CDS             complement(1141..3930)
                     /locus_tag="PK218_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97480.1"
                     /translation="MKKTVLNFLLCSFFVFLLSTNGRAQNVFSGEPVQVVGSFNGYST
                     TPYNSDYRTSAYRKVATTTSNPNDGRGQWATTINIQNSGGDATPINMTGGGGNGFLFI
                     SGPSGNRFQNKWVFSGIGSGSVNAINGISAFNSGNDMGLNMNTTGRYTFVFNDVGYTN
                     INAKYYVGYTSNTPVTLSNGGVTYSGGQPIISITTSAAPSSGENIYVRYRTASNDFTS
                     NTAVVQATGSGTSWSATLPTQTCGETVYYYIYTSTRTLVQINADSETDRTLATLRYED
                     NSGSNFSFTVSPVPTAGITNNSGTTVLSCTQTSISVTATGGTSYSWNNGLGAGASKNI
                     TSSGNYTVTVSSANGCTASASIVVSGVVTPQTNYYLDADNDGFGSGVPIAACSNPSPG
                     MYVTQNGDCDDTKNTVYPGATEVCWNNILENCNGTMSQGCSPIIVNMATPNNSTLPSF
                     ATAVAAYPYDYPGATTKEYRFSIKNNQTNVTEEVISATRFAAIPVSIRNYNISYEIKV
                     SAIIDGELVPYSGSTITVFSPIVALAKLSPTSCNATLNTLYSTISSNAMFGAITYKFR
                     VRLTSDNGPLPTYYYSNASSSRFTTLNSFTGLLTQYNTSYTMAVQYEFMDIITGFPTL
                     SGYGEECTITTPNVPLIGLNTPICGSNVTSLGASISATPASGALQYEFRIRESSDNGP
                     TPTYYFTNANASRFSTLSAFQGIVYSYVTQYSISVRYKINFNGSEIWSNFGPECTITT
                     PVFPVTEVQPDQCGIESATLDQSFNIVPYPGFPMYKITLWEQNGEELLLVGTIFRNVS
                     NFKLNMFTGTELNKSYSISVSIKINNSFGPYGKSCDIGTFPAVVERAGVTPFNVFSFP
                     NPFSNQFTLKLESSSKSIVTIKVYDLVGKLIEDRECKASEIEYQFIGANYSSGIYNVI
                     VSQDANTKNFRMIKR"
     gene            complement(4197..5243)
                     /gene="rfbB"
                     /locus_tag="PK218_02865"
     CDS             complement(4197..5243)
                     /gene="rfbB"
                     /locus_tag="PK218_02865"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPW97481.1"
                     /translation="MKKIVITGGAGFIGSHVVRRFVNKYPDYKIYNLDALTYAGNLEN
                     IADIENKPNYTFVKGDITDADFINELFQKEQFDSVIHLAAESHVDRSITDPLAFVKTN
                     VIGTMNLLNAAKTIWKNNFVDKRFYHVSTDEVYGSLGATGLFTETTPYDPNSPYSASK
                     ASSDHFVRAYGETYGLPYVITNCSNNYGPNHFPEKLIPLFINNIINHKPLPVYGDGKY
                     TRDWLYVIDHAIAIDLVFHDGKNHETYNIGGFNEWQNIELVQLLCQKMDQKLGRKPGE
                     SNSLITYVKDRPGHDLRYAIDASKINKELGWKPSVTFEEGLSKTIDWYLSNEKWLKNV
                     TSGDYQNYYSNQYE"
     gene            complement(5243..6520)
                     /locus_tag="PK218_02870"
     CDS             complement(5243..6520)
                     /locus_tag="PK218_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPW97482.1"
                     /translation="MGIKIAVIGLGYVGLPLARLFATKYPVVGFDINEKRIKELNSGI
                     DATLEVDEAQLKAVLVTSSTLDNGLYCSNILNDLNDCNYYIVTVPTPVDKNNRPDLTP
                     LYKSSETVGKVLKKGDIVIYESTVYPGVTEEECIPVLEKVSGLKFNVDFYAGYSPERI
                     NPGDKEHTVDKILKVTSGSTPEIGQKVDELYKSVITAGTHLASSIKVAEAAKVIENSQ
                     RDINIAFVNELAKIFNLLEIDTHAVLEAAGTKWNFLPFKPGLVGGHCIGVDPYYLAQK
                     AQEKGYHPEIILAGRRLNDSMGDYVANQVIKMMIKKGIKVNGSKILMLGIAFKENCPD
                     VRNTKVVDVVKALQDFETEITIYDPWANPEEVKHEYGLETICELPNQQYDAVVLAVSH
                     NEFKALDFNKLKKDIGIVFDVKGVLNEKADARL"
     gene            complement(6576..7967)
                     /locus_tag="PK218_02875"
     CDS             complement(6576..7967)
                     /locus_tag="PK218_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HPW97483.1"
                     /translation="MKITNICCIGAGYVGGPTMAVIAAKCPHIKVTVVDLNEKRIADW
                     NNPDINKIPIYEPGLNEIVEKSRGKNLFFSTEVDKAIDEAQLIFISVNTPTKTYGTGK
                     GMAADLKHIELCARQIAKVSKTDKIIVEKSTLPVRTAEAIKSILDNTGNGVQFQILSN
                     PEFLAEGTAVQDLLNPDRVLIGGETTPKGDEAIASLVEVYANWVPEERILKTNVWSSE
                     LSKLTANAFLAQRVSSINAISELCEKTEANVNEVAKAIGMDSRIGSKFLKASVGFGGS
                     CFQKDILNLVYISKAFGLNEVADYWEQVIIMNDHQKRRFSKNIVQTLYNTVSGKKIAF
                     LGWAFKKDTNDTRESAAIYVADDLINEQAKIALYDPKVERPQVLSDLNYLETRCSSQN
                     EDSIQNFKNPYEACENSHAIAILTEWDEFKEYDWQKIYNIMLKPAFIFDGRNLLDAKK
                     MNEIGFVYQSIGS"
     gene            complement(7973..8950)
                     /locus_tag="PK218_02880"
     CDS             complement(7973..8950)
                     /locus_tag="PK218_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW97484.1"
                     /translation="MSNQKTILITGGAGFIGTNLCEYFLQKEYKVVCLDNFATGHKRN
                     IIPFLENKNFKLIEGDIRDSKTCIKAVENVEYVLHQAALGSVPRSIKDPSTTNEVNIN
                     GFLNMLIAAKDANVKRFVYAASSSTYGDSESLPKIEDTIGKPLSPYAITKYVNELYAE
                     IFSKTYGIETIGLRYFNVFGRKQDPNGAYAAVIPKFVTQLINHESPIINGDGNYSRDF
                     TYIDNVIQMNELAMLTQNPKAINTVYNTAFGDRTTLNDLMKFLKQYLSEFDTEISKIE
                     VIHGPNRTGDIPHSLASIEKAKELLNYNPQYSFQDGLKEAVKWYWENLK"
     gene            complement(8950..11418)
                     /locus_tag="PK218_02885"
     CDS             complement(8950..11418)
                     /locus_tag="PK218_02885"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPW97485.1"
                     /translation="MLDVKDFSLFEKQNNFDFKSFLIKIVSYWKWFIVSLAITFFIAY
                     QVNIRKQKIFGIDTTIGVSDENNPLFTSNTSLVFNWGGTSDKVQSIATTFKSRSHNEM
                     VVDSLQFYTDYFIKTDYYYKDVYGQIPFKVTIDKSKFQLYNQFVKFKMTSSNTFQISI
                     PFTQSSVEVIKYADGETKFVSVNPKEFVANYRVNQVVDLPFLSFILTINDKVALKTNQ
                     EVLVRFNSFNQTVSKYRKINISIDEKAASILRLSLEGTNKERMVDYLNSTVEILKKKQ
                     LDNKNLFAKNTIAFIDETLSSMENQLKDSGNELKDFSRNNNVLDIQDKGAQFKGQLLE
                     YDVKKDEVERKIAYLNTLKNYLTKSVDFSKLPAPTVAGIEEPNIVNNVAKIIEFSIKR
                     SDLKYTSKGNIFYQRLDSEIESIKKVLLENINSLRLALQYDLDKVNSKLAEIESDIRK
                     LPESSQQYLKISRKYNLSDNIYNTYLQKRSEASIVKAANLSDIKFIDPAKDVGEGLLG
                     PKTKVNYVLALLIGIIIPLVIIFLFFFINNSIQNIEDILSVTKIPLIGIVGIKHNENN
                     LSVFERPKSALSESFRAIRSSLQFLYKKQSKDGAKTLMLTSSISGEGKTFCSINIATV
                     FALSEKKTIILGLDLRKPKIFDDFNIRNEIGAVNYLIGQKTLDEVIQKTHIPYLDVIT
                     SGPIPPNPSELILSEAMKEMMNDLKKEYDYIILDTPPVGLVSDALEIAQYSEVILYVV
                     RQNFTKKEMLTLLNNRTKRGELNNISIIFNGYENKAKYGTGYGYGYGYGYGYGYGYSN
                     GYHEEEEPKGFFNKLLFKLFKKQK"
     gene            complement(11429..12178)
                     /locus_tag="PK218_02890"
     CDS             complement(11429..12178)
                     /locus_tag="PK218_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPW97486.1"
                     /translation="MFTSCIPTNELIYLQNNNQKKENQSVEQIVNKPYRIQTNDILSI
                     SIKAIDPKLVEIFNVTATGSTSGSSEQAIYFNGYVVDDHGNIRFPILGEINVLGKTTE
                     EIREEVEGRLLDQYFKEEAGIFVSVKLSGFRYTINGEVGSTGTKVIFQDKVNILEAIA
                     NAGDIEVTGNRKDVIVMRRFPQGNETYHIDLTSSEAIHSPVYYLQPNDYIYVKPLKQK
                     TWGTGRTGVESISTIITLLSLATTTYLLLKR"
     gene            complement(12295..12918)
                     /gene="recR"
                     /locus_tag="PK218_02895"
     CDS             complement(12295..12918)
                     /gene="recR"
                     /locus_tag="PK218_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPW97487.1"
                     /translation="MEFSSKLLEKAVNEVAQLPGIGKRTALRLVLHLLKQPMEQTQFL
                     SNALTTMREEIKFCKSCHNISDVEVCEICNNGARNHQIICVVEDVRDVMAIENTNQFK
                     GIYHVLGGKISPIDGVGPSQLNINSLIEKAKLGNIEELIFALSSTMEGDTTNFYIFKH
                     IKDLPIKTSTIARGIAVGDELEYADEVTLGRSILNRIPFENSVNKMH"
     gene            13005..14540
                     /locus_tag="PK218_02900"
     CDS             13005..14540
                     /locus_tag="PK218_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPW97488.1"
                     /translation="MTPITILIIIIIYFGLLLFISNVVSKKGSDNDTFFKANKNSKWY
                     LVAFGMIGTALSGVTFISVPGEVGNPDLQFKYFQFVLGNALGIIVVATVLLPLYYRMN
                     LTSIYSYIEKRLGIISYKTAATIFLISRTIGSAFRLYLVVIVLQKFVFDNFGIPFAVT
                     VLLSLAFIFAYTYRGGLKTIIITDTLQTFFLVSSVFLTIIFICNSLDLSFVQAFETVK
                     DSNYSKIFFFDDFLNRNYFWKQLLGGMFVTIAMVGLDQDLMQKNLSCKNIGEAQKNMF
                     VFTGIFVIINIFFLSVGALLYIYAEKNGISIPMVDGSPRTDLLFPEIAFHHLSLIPAV
                     IFLLGLTAATFATTDSALTALTTSFCVDFLGMDKAENFKEATPKSKVEIEIRNKKLVK
                     TRHLVHIGFSFLMFLVIIIFNSLNDKSVVTMIFKVASYTYGPLLGLYAFGLLMQTKTV
                     HDKFVPLICMISPAICFFIVKYSETLLGKYAIDNELIIVNGFITFLGLLSISKPATEN
                     TRF"
     gene            complement(14537..14902)
                     /locus_tag="PK218_02905"
     CDS             complement(14537..14902)
                     /locus_tag="PK218_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPW97489.1"
                     /translation="MKNKKTLVLGASTKPDRYAFKAITKLVDNGHSVIALGQNTGEVA
                     GIKIYTKNIPLKNIDTVTLYLNPLRQRDYYNYIIETKPKRVIFNPGTENPEFYQLLKS
                     NGIEVEVACTLVLLATNQY"
     gene            15011..15634
                     /locus_tag="PK218_02910"
     CDS             15011..15634
                     /locus_tag="PK218_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family NAAT transporter"
                     /protein_id="HPW97490.1"
                     /translation="MEVFLILLGALFSVLNPIGTVPIFVSLTQDFTKKERSNLSLRTA
                     INVFIILIISFFIGQYVLTFFGITIPALRIAGGIIIASSGFSLLNGKFSKNRGINKKV
                     EEDISTRNAIALTPLAMPMLAGPGSISLLIAFHQEHDATFEVFMSSLSILVVSIVIFF
                     ILRSAHYLAKILGASGIVAVSRIIGFLTISIGIQYIITAVTDIFVKN"
     gene            complement(15631..>16560)
                     /locus_tag="PK218_02915"
     CDS             complement(15631..>16560)
                     /locus_tag="PK218_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HPW97491.1"
                     /translation="QTTNSILMIRPVAFRMNEQTAVNNYYQKVLDGLSPETVNEKAQK
                     EFDDFVAKLQKVGINVIVVDDTLEPNTPDSIFPNNWISFHQNGDVVLYPMFAENRRAE
                     RREDILDILEDEGFQINEIMDYTSAEEDAIFLEGTGSLLLDRENGKAYCALSPRADEE
                     LMIEFCEDFELSPVIFEAYQTVNGERKLIYHTNVMMCLGDTFAVICADCIDDKKERKM
                     VLDSLRRDEKEIILITEDQMNNFAGNMLELKGKNDERFLIMSESAYKSLTKKQIGQLE
                     AHVSIIHSSLDTIEACGGGSARCMMAEIFLPRN"
     assembly_gap    16561..16660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16759..17019)
                     /locus_tag="PK218_02920"
     CDS             complement(16759..17019)
                     /locus_tag="PK218_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPW97492.1"
                     /translation="MRRSSDSIVSNIVEGYGRKRYKADFIKFLVYSLSSCLETKNHLF
                     KISVLYHGTIDNIDELISSYDNLGGKIFNFIVYVEKKLENLI"
     gene            complement(17192..18106)
                     /locus_tag="PK218_02925"
     CDS             complement(17192..18106)
                     /locus_tag="PK218_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HPW97493.1"
                     /translation="MLQLNVKNETSRLRAVVLGSALNNGPTPSVEEAYDPKSLEHILA
                     GTYPIEEDMIKEMEAFNQVFQKYDVKVFRPEMIENYNQIFTRDIGFVIDDVFVKANIL
                     PDRNRELEAIQYVLDQINPSKIVRPPEEVHIEGGDVMLWNDYVFIGTYKGSDYKDYIT
                     ARTNMEGVNYIKALFPNKIVKEFDLVKSKLEARDNALHLDCCFQPVGKNKGIIYKSGF
                     REEADYVYLVKLFGKENLFHITRDEMYNMNSNVFSIDENVVVSEKNFTRLNAWLRNNG
                     FIVEEIPYAEIAKQEGLLRCSTLPLIRD"
     gene            complement(18180..18656)
                     /locus_tag="PK218_02930"
     CDS             complement(18180..18656)
                     /locus_tag="PK218_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97494.1"
                     /translation="MIKKILKRCKIISIDMLFYSMNASEYKPCTVLGFDIQEDKSNPN
                     KIEYFIKVEGKKVHQSYLFKSIDILKLIHKKGPVIGECATISEFKGKSIYPYVINYIA
                     KKELFEKKTDEVFITVNANNLASIKGIEKAGFTLKSSIKAKRFLVFYFQKQIKIYK"
     gene            complement(18653..19717)
                     /locus_tag="PK218_02935"
     CDS             complement(18653..19717)
                     /locus_tag="PK218_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPW97495.1"
                     /translation="MKILFTNDPKWLKKWDDYIVTEDRASHLMLSDWNKSFESYGFEF
                     EICLALENDKIIGGFAAVIAKAMVFKFYIVPFGPIISSDYEEKLNEIIESVYNRAKKR
                     NCCYAQINLPFSDIDNVHLLTNSLKIPFLEKAAKGHLFKYVYSSSGLNWVDLKHLDEE
                     KMIMSLKPSVRRNVRNSYRKELEFKVLDTQEGIENAYRLFLENATNANYTIRNWEDIK
                     NTLFGLNEKGLLKILSVFKSNEIKGAILLVKSGNYYTYILGGSKKETPDLRTGDFLQW
                     EAIKLSINEGLDGYNISLGGSKGVFEFKNSFNTTHYYIQDGQYHWILKPIYFKFYTIV
                     EKKLKKHKKRIANLLSVFKK"
     gene            complement(19719..21323)
                     /locus_tag="PK218_02940"
     CDS             complement(19719..21323)
                     /locus_tag="PK218_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97496.1"
                     /translation="MVQSLKLIFSNKSEHLDVAFFEKKLLIPLQNQKVSECNTFDIEI
                     TFKDEINLFRNHDYQWIKCDRELIASVFTPKIIKLNNGFFVQPNIQSGIWEFNPKFKN
                     KIRWRFNPEHAASITKYTGKKNEKIVQPVTANFDFSVELALLFSDKNAVEFSRSPIPF
                     SAIACFTDHCDFDTQENLELQRVFFEQNNIKITKGFFLNHFSKRNENASFENNSTELL
                     KWQKAGHELCYHSLSQSLKHNKESFEDFESFQPPIEVVPTWIDHGYQPYNFSFFENLG
                     FEQEKFAAILNKNKITTLWNYIDSGTATLGVLNQLNSNDFTLKSYFAGIQDLKFKDKM
                     ALKIKIILFHFYGDRETIYNFNRVKRIIKLKQYSKICLFTINLTIVFFKIIKVFLFWN
                     STKSKPFKLAKYLPLVFKHRFQNSTFNIFQTVKIIDFRKALNKRNIDKLVNEKGIFIA
                     HTYFSDSMKHHNGKLFKTPTTIDESVAQNFSYLSKKINEKTIWNPTLNELVLFLNKFE
                     AIKLDVDKSGNIINLLHSDLPFRELY"
     gene            complement(21434..22231)
                     /locus_tag="PK218_02945"
     CDS             complement(21434..22231)
                     /locus_tag="PK218_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPW97497.1"
                     /translation="MKLKKKIKKTISFFIPKGNSKEKIKLFYYKLFSNKAISFDIKID
                     NGKVIYKTTFDNKVLYTNEALYSIAVDFGYYQHNYKAKNGDFVIDAGANVGHISMFFS
                     KCVGQNGKVFCFEPDKHNIKSLENNIKLNSDLKDNIIIQDMLLWNEDTLLDFEESGTV
                     ASSAKWFSENANIVKKQAVTIDNWVKNNNVHRLDLIKMDIEGAEIEAIEGCVETIKTL
                     KPKFAIASYHIIDNQPTYIKVEEFFKKMNYPFKTVRFRGNEIITFAG"
     gene            complement(22315..24279)
                     /locus_tag="PK218_02950"
     CDS             complement(22315..24279)
                     /locus_tag="PK218_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97498.1"
                     /translation="MKIVKLLLFCFIWLSSITSFAQVEDYLKLEQNRITKAQNIEKEV
                     QAFIVKNIGKYKLTNEIEIETIQHLNDEIEELSIKEIEVAIEKAKVVELRKLFFKTFP
                     DKKKEYIANPISSSLRQSCVNGDFEDGTASYSFWSDAHPQPASGSSFFQSCGTPTVVT
                     GTNVVTPSVNNFNSTITLIDNTAPNYQQFDPVLASFGQNIPTLNTNSGSKCIKLNNTQ
                     GYGSSDLTTMSRYMPSINQPTIDFNFSLVMDNKVAHGQDIQPYFRVRVLDQNNNVVDQ
                     ICIIADPENCLFNRINVSNSRRILYTGWVCARLNVQDILNQPGTIEFSVSDCQPSQHF
                     GTVYIDNLCGFVCATPQLGALSLDPTNINCPNATPEVPVNVCGTYQPPVNASLNTLSL
                     NIIQNGNTITTINAPTQLTSDTFCFSFLPSAFGANPSGSFEFEVNATFNVACPAGTYI
                     YDMTDNSANVGPDVTFIDCCLPTLILIAPADNVTNLDATTATQKERSDWIKATNIVEV
                     GDAVFQNGVVYHAGNYVELNPGFEAVTGSQFSAYIEGCSSNYIYRNSDTNSSSTEEGL
                     LEELNVTKLNIEFLIVPNPSSSSIELIMKGNQFRNISITTIDGKMVFDQNIELTDKAR
                     VDVSRYANGVYIVNLLTDDGDVYSKKLIKN"
     gene            complement(24281..>25143)
                     /locus_tag="PK218_02955"
     CDS             complement(24281..>25143)
                     /locus_tag="PK218_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97499.1"
                     /translation="LPTFTNTDSQSLTLNGNTISISGGNTITLPTLTDTDQQTLALNG
                     NVLSILNGNSVTLPTFTNTDSQSLTLNGNTLSISGGNSITIPTPTFAVIPGTNTTISG
                     DGSTTSPFQINAIDTSLYANDGAINQATTTNNNRKVNLNNRNLWFDTTNSTSNGKLYI
                     GSTPNFPSTSGNYKLFVEGGIMTEKVKVALRNTSNWADYVFEKDYKLLPLNEVEKFVK
                     INKHLPGVQSAKELAENGIDVAEMQSKQMEKIEELTLYIIEQNKKLEKQNDEIQTLKT
                     QMSLLIQKKK"
     assembly_gap    25144..25243
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25244..26550)
                     /locus_tag="PK218_02960"
     CDS             complement(<25244..26550)
                     /locus_tag="PK218_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97500.1"
                     /translation="MKIKNYFVVGSFLVLNFSTVIGQTANTFFGVESGTNNSGNFNSG
                     YGTNSLINNSSHSNNAFGFNTLIQTTGGYNCAFGNDALKLNTSGTLNVALGTRTLENN
                     ATGSGNIAIGHRSLGSNTSGNRNTAVGYGTLVKVNGNDNIALGYQTPRSLISGNSNIF
                     IGNENGLNLTNGNQNVFIGSVRLNNTVSSNRIAGNSLDRSIILADGAGNQRIFISRIG
                     NMGLGLGNNIIPINRLDVNGGVVIGKNYVPSNSDTGSYVAPVNGLLVEGKVGLGNSNP
                     NNKLEITHGTSGNSGLRFTNLTSSYVPTSSQTSDKFLSVNQNGDVILQKMANPSITNG
                     LSSTGNVMNSFVNGSNANAPIVNTISNSFNTNNQLVTTVNGVSSAPITLPIPDFSEID
                     ESVTNEIQVLTLNGNTVSLSLGGGSFTLPTLTDTDQQTLALNG"
     gene            complement(26788..28074)
                     /locus_tag="PK218_02965"
     CDS             complement(26788..28074)
                     /locus_tag="PK218_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97501.1"
                     /translation="MKKRIIDFLAKNPVFITALVCVLFYFLPFKPKPFGDGDFHFGTI
                     QLIEFIKNGFHGEIMINKGFLTLFCYLIPYGLVYSFHNDALYLFSGVAFSCVFVCLSI
                     FYLFKAFTILGFSNQSKAFTLLILCLFPIHVYYAMGIIGEIFAFFAVSVMIYFWVKIN
                     FSETSKTSDFVYLALAFVLLYGIKPTALPFLIAFGLYILFLSYTTKNKTIFLLTLTII
                     PLLILTEVKLDKNDNEFKKKVFRNQILWSRFELRDEPFNWLPQHGNDSFASNDYKNNL
                     RKRYELDSICLTNKYDRTQYYIKWVKDDIVNHPFITLRQYFLKFFQSQSFIITPLMKS
                     HKPVWFKYGIHIYINLINSILVIIGLITMYKLFKRKKWRFFIPILLYWGSALVYVFLF
                     HSEQRYMFPFRPMLIFLFAYYIANRNLIIDNKSFAK"
     gene            complement(28055..28987)
                     /locus_tag="PK218_02970"
     CDS             complement(28055..28987)
                     /locus_tag="PK218_02970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW97502.1"
                     /translation="MKLSFVIPVYRNEGSLIPTFTKIKDLVTKNKFEYELIFVNDGSD
                     DNSFEELKELFKIDSNVKVLSFSRNFGQVAAVIAGLKEVTGDVVISMSADLQEPIELI
                     EQMVQKWQEGNEIVICHRTDREDGFIANNTSKIFYKLMKYVNPKMPNGGFDFLLLDKK
                     AVNVLNQIDERNRFFQGDVLWLGFNTAFIPYRRIKRAIGKSQWTLSKKIKYFIDGLLN
                     TSYVPIRLMSFLGLCFSFVGFLYSLIIIYSRFVNNTPFNGWAPIMILLLIIGGMIMVM
                     LGIIGEYVWRAYDETRKRPIYIIKDKFVNEEENN"
     gene            complement(29064..30347)
                     /locus_tag="PK218_02975"
     CDS             complement(29064..30347)
                     /locus_tag="PK218_02975"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPW97503.1"
                     /translation="MSKIATLELDGKKYEFPLVTGSENETAIDIEKLRALTGAITLDP
                     GYKNSGSCKSDITFLDGEEGILRYRGYSIESLAESANFLEVSYLVIFGELPTKEQLAK
                     FESDIHNYTLVNEEMKNIVDGFPKTAHPMGVLASLTSALTAFNPKVVNVENEKEMYDA
                     VCKTMAKFLVIATWTYRKIKGFPLNNYDNTKGYVENFMRLMFELPTEPYKYNQTVIDA
                     LDKLFILHADHEQNCSTSTVRIVGSSHAGLFASISAGVSALWGPLHGGANQAVLEMLE
                     AIQADGGDADKYMAKAKDKNDPFRLMGFGHRVYKNFDPRARIIKKAADEVLNSLGVDD
                     PILDIAKKLEAAALVDEYFVSRKLYPNVDFYSGIIYRALGIPTDMFTVMFAIGRLPGW
                     IAQWKEMRLNNEPIGRPRQVYTGYPLREFVPLEKR"
     gene            complement(30982..>31773)
                     /locus_tag="PK218_02980"
     CDS             complement(30982..>31773)
                     /locus_tag="PK218_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97504.1"
                     /translation="TLNVNAPTETPAVNIGDVLVFTSFIDISTDENWVDNAFTLNQTV
                     VGSFDPNDITCLEGDIVSPTQIGNYLHYNIRFENTGTAPAENIVVKIDVNPADFDINS
                     LQLMNTSHPVEARIRGNIVEFIFQNIMLDSGGHGNVLLKIRSQPNLIQGDLVAKKANI
                     YFDYNFPIETNEADTVFQALNNPSFEQDNSISIYPNPSSSIINIKSNFNIKTIELYDV
                     QGRLLQTNIINNSETTFDITSKSKGIYFLKIVSEKGIKVEKLVKE"
BASE COUNT        10198 a         5204 c         4516 g        11655 t
ORIGIN      
        1 ttcaatgaag attcactcac tgaaaaagtt cctgttgttc cagtggtcga tgaatcaaca
       61 actcctgtac cgccaatcaa tgggttagta ttcgttgctg aggcagcata tacaattcct
      121 cttcctgtaa cagcactacc accatcactt gtgacatttc caccaagagt tgccgatgtt
      181 gttcctatag aacttggtgt aagatctgta gtaacaactg gtgcagttac aactgcagct
      241 aaagtgccat taaatattaa attaccttga agtccacctg ttccaccagt tgattctgac
      301 ccataagcat aaactctaaa agtaattgag gtaacaccag ttgcagaaga agttgcagta
      361 ttgcctgtat atccgttatt ggcatctgga atttgtagaa cattagaagt tatagaaaca
      421 cttccattac tagaggttaa tggcaaaaga gttgcataat tatctacact gcttctccat
      481 tgaaataatc ttggaccagt cgaacttctt cctgctccaa aatttatact catattagat
      541 aaagtatatc cactagttgc tgatatagta aattgaaaat atttactcaa gttaacacta
      601 cctccagcag cacctccatc agtggatcca gtatcccaat catttgctct aaaattacca
      661 gaacttgaag aggtggttac tccttcttta gttaatggcg atactgttag attagttata
      721 gtcgaaccgt tataggcaaa actactcaca tttccactag atcctgtgaa ggtatatgtt
      781 ccagtaaaag cttgaccaac gctattgctg ggcaataaca aaaacaccaa agaaaaacac
      841 caaaaagtga gatgcaatcc ttttttaaat aataattttc ttttcataat taatatcgtt
      901 ttattttttg actgtaacaa aattatacaa attattcctt ttaaaagcaa taaaacttta
      961 actaattgtt aactattcaa ccatttagct ttaaacgatt attttcgctt ttaatcgata
     1021 aaaaatattg agttataaac actcatttct atttttgttg aaaaagaatt ataatatcaa
     1081 ttcttaaaaa acaaaaaacc tatcaatttc ttgataggtt tcaataaata gttggttatt
     1141 ttatcttttt atcattctga aattttttgt atttgcatct tgagatacta tgacattata
     1201 gattccactt gagtaatttg caccaataaa ttggtattct atttctgaag ctttgcattc
     1261 tctatcctca attagctttc caactaaatc ataaacttta atagtaacaa tagatttact
     1321 agaactttca agcttcaagg taaattgatt gctaaaagga tttggaaatg aaaacacatt
     1381 aaatggtgtc actccagctc tttcaacaac tgctggaaaa gttcctatat cacaactctt
     1441 tccgtaaggt ccaaatgagt tgttaatttt aattgaaaca gaaatgctat aactcttatt
     1501 caattctgta cctgtaaaca tatttaattt aaagttggat acgtttctaa aaatagttcc
     1561 tactaacaat aattcttcac cattctgttc ccaaagtgta attttataca ttggaaatcc
     1621 tggataggga acaatgttaa aactttggtc tagagttgca gattctatac cacattgatc
     1681 gggctgtact tctgtaactg gaaacactgg tgttgttatt gtgcattctg gcccaaagtt
     1741 tgaccatatc tcagaaccat taaaattaat tttgtagcga acagaaattg aatattgcgt
     1801 tacataagaa tacacaattc cttgaaacgc acttaacgtt gaaaatcgac ttgcattggc
     1861 attggtaaaa taatatgttg gtgttggtcc attatcgctt gattctctaa ttctaaattc
     1921 atattgtaag gcaccagaag ctggcgtagc agaaattgaa gcacctaaac ttgtaacatt
     1981 tgaaccacat ataggcgtat ttagaccaat taaaggaaca tttggcgttg ttattgtaca
     2041 ttcttcacca tatccagata atgtaggaaa accagtaata atatccataa attcatactg
     2101 aactgccata gtataactgg tattatattg agttaataaa ccagtaaaag aatttaatgt
     2161 ggtaaatcga gatgaagaag catttgaata gtagtaagtt ggcaatggtc cattatcgct
     2221 tgtgagtcta actctaaatt tataggtaat tgcaccaaac atggcgtttg aagaaatagt
     2281 actatacaaa gtattcaatg tggcattaca acttgttgga gacaattttg ctaatgcaac
     2341 aattggacta aaaacagtaa ttgttgagcc tgaatatgga accaattcac catcaattat
     2401 tgcagagact ttaatttcat aactaatatt ataatttcta attgataccg gaatagcagc
     2461 aaatcgagta gcagatataa cttcttcggt gacattagtt tgattatttt ttatagaaaa
     2521 tctatattct tttgtagttg ccccaggata atcataggga taagcagcta ctgctgtggc
     2581 aaatgatggt aaagtactat tattgggagt tgccatattt actattatcg gagaacaacc
     2641 ttgcgacatt gttccgttac aattttctaa aatattgttc caacaaactt ctgttgctcc
     2701 gggataaact gtattttttg tatcatcaca atctccattt tgagttacat acattcctgg
     2761 tgaaggattg gaacaggcag caattggcac gccactacca aaaccatcat tatctgcatc
     2821 taaataatag ttggtttgag gagtaacaac acccgaaact acaattgaag cgctagcagt
     2881 acatccattc gcgctggaaa ctgttacggt ataatttccc gaagaggtta tgttcttaga
     2941 tgctccagca cccaaaccat tgttccaaga ataactagtt ccaccagtag ctgttacact
     3001 aatacttgtt tgtgtacaag acaaaactgt agttccacta ttgttagtga ttcctgctgt
     3061 tggaacagga cttactgtaa atgaaaaatt agaacctgaa ttatcttcgt atctaagtgt
     3121 agccaacgtt ctatctgttt cactatcagc attaatttga actaatgttc ttgtacttgt
     3181 gtatatataa taataaactg tttcaccaca ggtttgagtt ggcaatgtag cagaccaaga
     3241 tgttcccgaa ccagtagctt gaactactgc tgtgttagaa gtaaaatcat tactagctgt
     3301 tctatatcta acatatatat tttctccaga agatggggct gcacttgtag taatagaaat
     3361 tattggctgt ccacccgaat aggtaactcc tccgttggaa agtgttactg gtgtatttga
     3421 agtgtaacct acataatatt tagcattgat attagtatat cctacatcat taaacacaaa
     3481 agtataacga ccagtagtat tcatattcag tcccatatca ttaccgctat taaaagcaga
     3541 aattccatta atggcattta cagatcctga acctatacct gaaaaaaccc atttattttg
     3601 aaaacgattt cctgatggtc ccgaaataaa taaaaatcca tttccacctc ctccagtcat
     3661 attgatagga gtagcatctc ctccagaatt ctgaatgttt atagtagtag cccattgtcc
     3721 tcttccatca tttggattac ttgtggtagt agctactttt cggtaagcac ttgttctgta
     3781 atcggaatta taaggtgtgg ttgaatatcc attaaacgaa ccaacaactt gaacaggttc
     3841 accactaaaa acgttttgtg ctctaccatt cgtggacaat agaaatacaa aaaaagaaca
     3901 caaaagaaaa ttaagtactg tttttttcat aattattgac tattaaatta atgatttaac
     3961 aatttttgaa ttaaataagt tatatatctt tttttttcaa tttaacataa aaaattattt
     4021 aaaattaaat aaatatctta ataagtaatt tttatttaaa gaaaacctgc caacgaaaac
     4081 gttttcgtgt tgataaataa caacattata aaaatagaaa aaaagaaaat acgcatagaa
     4141 aacaattaaa taatttctat gcgtatttaa caaaggacta gctttagact ttatgtttat
     4201 tcatattgat tactgtaata attttgataa tctcccgaag tcacattttt taaccatttt
     4261 tcattagaaa gataccaatc aatagtttta gacaatcctt cttcaaaagt gaccgaaggt
     4321 ttccaaccta attctttatt tattttagat gcatcaattg catagcgtaa atcgtgacca
     4381 ggtctatctt taacataagt tattaaacta ttagattctc ctggttttct acctagtttt
     4441 tgatccattt tttgacaaag taattgaacc aattcaatat tttgccattc attaaaaccg
     4501 ccaatattgt atgtttcatg attttttcca tcgtgaaaaa ccaaatcaat agcaatagca
     4561 tgatcaatta catacaacca atcacgcgta tattttccgt ctccatagac tggcagtggc
     4621 ttatgattta taatattatt tatgaataat ggaattaact tttctggaaa atgatttggt
     4681 ccataattgt ttgagcaatt ggttataaca tagggtaaac cataggtttc tccataggct
     4741 ctaacaaaat gatctgaact tgcttttgaa gccgaataag gtgaatttgg gtcatacggt
     4801 gttgtttcgg taaacaatcc tgttgcgcct aaacttccat aaacttcatc tgttgaaacg
     4861 tgataaaaac gtttgtcaac aaaattattt ttccaaatcg ttttcgcagc atttagcaaa
     4921 ttcatggttc caataacatt ggtttttaca aaagctagag gatcggttat agaacgatct
     4981 acatgtgatt ctgcagccaa atgaattacg ctatcaaatt gttctttttg gaacaattcg
     5041 tttataaaat cagcatcagt tatatcacct tttacaaagg tatagtttgg tttgttttct
     5101 atatcggcaa tattttcaag attacccgca taagtaagcg catctaaatt atatatttta
     5161 taatctggat acttattcac aaatcttctg acaacatgag aacctataaa tcctgcacct
     5221 ccggttatta ctattttttt cattacaatc ttgcgtctgc tttttcgttt aacactcctt
     5281 taacgtcaaa tacaattcca atatctttct ttaatttatt aaaatcaagt gctttaaatt
     5341 cattatgaga gactgctaat accacggcat catattgctg atttggcaat tcacaaattg
     5401 tttctaaacc atattcgtgt ttcacttctt caggattagc ccatggatca taaatcgtta
     5461 tttcagtttc aaagtcttgt aatgctttta caacatcaac tactttagta tttctaacat
     5521 cggggcaatt ttctttgaaa gcaattccta acattaaaat tttagaccca ttcactttga
     5581 ttcctttttt aatcatcatt ttgatgactt ggttggcaac ataatcaccc atactatcgt
     5641 ttaatcttct tcccgccaaa attatttcgg gatgatatcc tttttcttga gctttttgag
     5701 ccaaatagta gggatcaacc ccaatacaat gtccgccaac taatcctggc ttaaagggta
     5761 aaaaattcca ttttgttcct gcagcttcca gaacagcatg cgtgtcaatt tcgagaagat
     5821 taaatatttt tgctaattca ttaacaaaag caatattaat gtcacgttga gagttttcga
     5881 taacttttgc tgcttcggct acttttatgg atgaagctaa atgggttcca gctgtaataa
     5941 ctgatttata taactcatca actttttgac caatttcggg agttgaacca gaggtaacct
     6001 ttaaaatttt atcaaccgta tgttctttgt cgcctggatt aattctttct ggtgaataac
     6061 ctgcataaaa atcgacattg aattttaaac cgcttacttt ttctagaaca ggaatacatt
     6121 cttcttcggt aacaccagga taaacggttg actcataaat aacaatatct ccttttttca
     6181 gtactttgcc tacggtttcg ctagatttat agagtggtgt taaatcaggt ctattgtttt
     6241 tatctactgg agttggaact gtaactatat agtaattaca atcatttaaa tcgtttaaaa
     6301 tattgctaca ataaagtccg ttatctagtg ttgatgatgt tactaaaaca gcttttagtt
     6361 gtgcttcatc aacttccaaa gtagcgtcta ttccgctatt taattctttg attctttttt
     6421 cattaatatc aaagcctaca acaggatatt ttgttgcaaa aagtcttgcc aatggcaaac
     6481 caacatatcc taatcctatt actgctattt taattcccat ttattagttt tgtttgttta
     6541 gatttttttg agtgtttaca aatataaata ttttattaag aaccaataga ttgatataca
     6601 aacccaattt cattcatctt tttagcatcc aataaatttc taccatcaaa gatgaatgca
     6661 ggttttaaca ttatattata gattttttgc caatcatatt ctttaaattc atcccattct
     6721 gttagaatag caattgcatg ggagttttca catgcttcat agggattttt aaaattttga
     6781 atactgtctt cattttggga agaacatctt gtttctaaat aattcaaatc tgaaagtact
     6841 tgaggtcgtt ctacttttgg gtcatataga gcgatttttg cttgttcatt aatcaaatca
     6901 tcggcaacat atattgctgc agattctctt gtgtcattag tgtctttttt aaaagcccaa
     6961 cccaaaaagg ctatcttttt tccagaaaca gtgttgtata atgtttgaac aatgtttttg
     7021 gaaaatcttc ttttttgatg gtcattcata atgatgactt gttcccaata atcggctact
     7081 tcatttaaac caaaggcttt agagatatat accaaattta atatatcttt ttgaaaacat
     7141 gagcctccaa atcctacaga agcttttaag aatttagagc cgattctact atccattcct
     7201 attgctttgg ctacttcatt aacattagct tctgtttttt cacatagttc agaaatagca
     7261 tttatagaag aaactctttg cgctaaaaaa gcatttgctg ttagttttga taattctgaa
     7321 gaccaaacat tggttttcaa gattctttct tctggtaccc aatttgcata aacttcaact
     7381 aatgatgcta ttgcttcatc accttttgga gttgtttcgc caccgattaa tactctatct
     7441 ggatttaata aatcttgaac ggctgttcct tctgcaagaa attctgggtt tgaaagaatt
     7501 tgaaactgaa caccatttcc agtattgtct aaaatgcttt ttattgcttc tgcagttctt
     7561 actggtaatg ttgatttttc aacaataatt ttatctgttt tcgaaacttt tgcaatttgt
     7621 ctggcgcaca attcgatgtg ttttaaatcg gcagccattc cttttcctgt gccataggtc
     7681 tttgttggtg tatttaccga aataaatatt aattgtgctt catcaattgc tttatctact
     7741 tctgttgaaa aaaatagatt cttgcctcta gatttttcaa caatttcatt taaacctggt
     7801 tcgtatattg gaattttatt gatgtcagga ttgttccaat ctgcaattct tttttcattc
     7861 aaatctacaa cggtaacttt aatgtgtgga catttagcag caattactgc cattgttggt
     7921 ccgccaacat aaccagcacc aatacaacat atatttgtaa tcttcattta ttttatttta
     7981 aattttccca ataccatttc actgcttctt ttaaaccatc ttgaaaagaa tattgaggat
     8041 tataatttaa caattctttt gctttttcta tacttgccaa cgaatgcggt atatcacctg
     8101 ttctgtttgg cccgtgaatt acttcaatct tactaatttc agtatcaaat tcggataaat
     8161 attgttttaa aaatttcatt aaatcattca aagtcgttct atctccaaaa gctgtattat
     8221 aaactgtatt tatggctttt ggattttgtg ttaacatcgc caattcattc atttgtatca
     8281 cattatcaat ataagtgaaa tctcgagaat aatttccgtc tccattaata attgggcttt
     8341 catgatttat cagttgcgtc acaaattttg gaattacagc agcataggct ccatttgggt
     8401 cttgttttct accaaaaaca ttaaaatatc gcaatcctat agtttcaatt ccataagttt
     8461 tgctaaatat ttctgcatac aattcattta catattttgt gatagcatat ggagaaagag
     8521 gtttcccaat tgtatcttca attttgggta aactttccga atcaccatag gttgatgaac
     8581 tagccgcata aacaaatctt ttcacatttg cgtctttagc agctattagc atgtttaaaa
     8641 acccatttat attcacttca tttgtagtag agggatcttt tattgagcgt ggcaccgaac
     8701 ctaacgcagc ttggtgtaac acatattcaa cattttctac tgcttttatg caggttttac
     8761 tatctctaat atcgccttcg attaatttga aatttttgtt ttctaaaaaa ggaataatat
     8821 ttcttttatg acctgttgca aaattatcta aacaaactac tttatattct ttttgcaaaa
     8881 agtactcgca aagatttgtt ccaataaatc ctgctccgcc tgttattaaa atagtttttt
     8941 gattagacat tatttttgtt ttttaaataa tttaaaaagt agtttattaa aaaatccttt
     9001 cggttcttcc tcttcatgat aaccgtttga atagccataa ccatatccgt agccataacc
     9061 gtagccgtag ccataaccag ttccgtattt ggctttgttt tcatagccat taaaaattat
     9121 actaatatta tttaattcac ctcttttggt tctattgttc aataaggtta gcatttcttt
     9181 ttttgtgaaa ttttgtctta caacatataa aatcacttcg ctatattgcg ctatttctaa
     9241 tgcgtccgac actaatccaa ctggaggtgt atctaaaata atataatcgt attctttttt
     9301 taaatcgttc atcatttctt tcattgcctc gcttaaaatc aattcggaag gatttggtgg
     9361 aattggtcca gaagtaataa cgtctaaata aggaatatgt gttttttgga taacttcgtc
     9421 tagagttttt tgaccaatca ggtaatttac agctccaatt tcatttctga tattaaaatc
     9481 atcaaatatt tttggttttc ttaaatccag tcctaaaata atggtctttt tttcactcaa
     9541 tgcaaaaacg gtcgcaatat ttatagaaca aaacgtttta ccttctccac taattgatga
     9601 agttaacatt aatgtttttg caccatcttt gctttgtttt ttatacaaaa actgaagcga
     9661 tgatcgaatg gctctaaaag attctgaaag ggctgatttt ggtctttcaa atactgaaag
     9721 gttattttca ttgtgtttta taccaactat tccaataagt ggaatttttg ttacacttaa
     9781 tatatcttct atgttttgga tggaattatt aataaagaaa aataaaaaaa ttattaccaa
     9841 cgggataata atacctatta ataatgccag aacgtagttt acttttgtct ttgggccaag
     9901 caatccttct ccaacatctt ttgcaggatc aataaattta atatcagata aatttgctgc
     9961 ttttacaata gatgcttcac ttcttttttg caagtaagta ttgtaaatat tatcgcttaa
    10021 attgtatttt ctagatattt ttaaatattg ctgtgaactt tcgggtaatt ttctaatatc
    10081 gctttctatt tccgctaatt tagaatttac tttatctaaa tcatattgta aagcaagtcg
    10141 aagtgaattg atattttcta ataaaacttt ttttatagat tcaatttcac tgtccaatcg
    10201 ttgataaaaa atgtttcctt ttgaagtata ttttaaatct gatcgtttaa tagaaaactc
    10261 gattatttta gcaacattat ttacaatatt tggctcttca attccagcaa cagttggtgc
    10321 tggaagtttg gaaaaatcga cactttttgt tagataattt tttaaggtgt ttagataagc
    10381 tattttgcgt tcaacttcat ctttttttac atcatactca agtaattgtc ctttaaattg
    10441 cgcaccttta tcctgaatat caagcacatt gttatttctg ctaaaatctt ttaactcgtt
    10501 tccagaatct tttaactgat tttccataga tgagagcgtt tcatctatga atgcgatagt
    10561 gttttttgca aataaatttt tgttgtctaa ttgctttttt ttcaaaattt caaccgtaga
    10621 gttcaaataa tccaccattc gttccttatt tgttccttct aagcttagtc ttaaaatcga
    10681 agctgctttt tcatcaatgc taatgtttat ctttcggtat ttactaacgg tctgattaaa
    10741 agaattaaat cgcaccaata cttcttgatt tgtttttaat gctactttat cgttaatcgt
    10801 caaaatgaaa ctcaaaaacg gcaaatctac aacttgattt actctataat tagcaacaaa
    10861 ctcttttgga tttactgaaa caaattttgt ttctccatca gcatatttaa taacttcaac
    10921 tgaactttga gtaaaaggaa tactaatttg aaaagtattt gaagaagtca ttttaaattt
    10981 tacaaactga ttgtagagtt gaaacttcga tttatcaatt gttactttaa atggaatttg
    11041 accataaaca tctttataat aataatccgt tttaataaaa taatcggtat aaaattgcag
    11101 actatctaca accatttcat tatgtgatct agatttaaaa gtggttgcaa tgctttgcac
    11161 tttatcggaa gttccacccc aattaaaaac caaactggta tttgaagtaa acaaagggtt
    11221 gttttcgtca gatactccaa tagtagtgtc aattccgaaa attttttgct ttctaatatt
    11281 aacctgataa gctataaaaa atgttattgc taaagaaact ataaaccatt tccaatagct
    11341 aacaattttt atcaaaaaac ttttgaaatc aaaattattt tgtttttcaa acaacgaaaa
    11401 atctttaacg tctaacattt aattataatt atcttttgag taataaatag gtagtagtgg
    11461 ccaatgaaag caaggtaata attgttgata tagattcaac acctgttcta cctgtacccc
    11521 atgttttttg tttcaatggt ttaacataaa tataatcatt aggttgtaaa taataaactg
    11581 gtgagtgtat tgcttcactt gaagttaaat cgatatgata cgtttcattt ccttgaggaa
    11641 atcttctcat tacgataaca tcttttctat ttcctgttac ctcaatatca ccagcattgg
    11701 caatagcttc taagatgttt actttatctt ggaaaataac ttttgttcct gtgcttccaa
    11761 cttcgccatt aatagtatat ctaaaaccac taagctttac actgacaaaa atgcctgcct
    11821 cttctttaaa atattggtcc aacaatctac cttctacctc ttctctaatt tcttctgttg
    11881 ttttacccaa aacatttatt tctcctaaga ttggaaatcg tatattgcca tgatcatcaa
    11941 caacataacc attaaaataa attgcctgct cactacttcc tgaagtagaa ccggtagctg
    12001 tgacattaaa aatttctacc aattttggat caattgcttt tatagaaata cttaatatat
    12061 catttgtctg aatacgatag ggcttgttga caatttgttc aacagattga ttttcttttt
    12121 tctgattatt attttgaagg taaatcaact cgttggtcgg gatacaagat gtaaacataa
    12181 gacctattat ggataatagc agtaaattat atttgttcat tagtttttaa aaacttgtta
    12241 gccaacaaag atagtttttc aattctaatt acaaaaatgt aaaaagtgtt tattttaatg
    12301 cattttgttt acagaatttt caaatggaat tcgattcaaa atacttctac ccagagtcac
    12361 ttcatcggcg tattctaatt cgtcgccaac agcaattcct cttgcaatgg tagatgtttt
    12421 tatgggtaaa tctttaatgt gtttaaaaat ataaaaatta gtcgtatcgc cttccattgt
    12481 ggaacttaaa gcaaatatca actcttcaat atttcctaat tttgcttttt ctattaagga
    12541 attaatattt aattgacttg gtccgacacc atcaatagga gaaatctttc cacccaaaac
    12601 atgataaatt cctttaaatt gatttgtgtt ttcaattgcc attacatcgc gaacatcttc
    12661 aactacgcaa ataatttgat gatttcttgc cccattatta caaatttcac aaacctcaac
    12721 atctgaaata ttatgacatg atttacaaaa cttaatttct tcacgcattg tggttaatgc
    12781 atttgacaaa aattgagttt gttccattgg ttgttttagc aaatgtaaaa ccaagcgcaa
    12841 tgccgtacgt ttgccaattc caggcaattg cgctacttca ttgaccgctt tttcaagtaa
    12901 ttttgatgaa aactccatag tttctaaaat tccaaaaaac aaattccaaa tcacaaagta
    12961 aattagatac gttgaatttt gtattttgca caaaaataca ttttatgacg ccaataacca
    13021 tacttatcat cattataatt tattttggat tattgctttt tatttcaaat gtagtttcca
    13081 aaaaaggaag tgataatgat acttttttta aagccaataa aaattcaaaa tggtatttag
    13141 tggcttttgg aatgatagga acagctcttt ctggcgttac ctttatttcg gttcctggtg
    13201 aagttggaaa tcctgatttg cagtttaaat attttcaatt tgtacttgga aatgcactcg
    13261 gaattattgt tgtagctact gtattgcttc ctttatatta ccgaatgaat ttgacttcca
    13321 tttacagtta tattgaaaaa agattgggga ttattagcta taaaaccgct gctactattt
    13381 ttctaatcag cagaacgatt ggttctgctt tccgattgta tttggtggtt atcgtgttgc
    13441 aaaaatttgt ttttgataat tttggaattc catttgccgt cactgttttg ctttcattgg
    13501 cattcatttt tgcttacact tatcgtggtg gtttaaaaac aattatcatt accgatacct
    13561 tgcaaacgtt tttcttagtt tcgtctgttt tcttaactat tatattcatt tgcaacagct
    13621 tagatttatc ttttgtgcaa gcatttgaaa ccgtaaaaga cagtaattat tcaaaaattt
    13681 tcttctttga tgatttctta aatcgtaatt atttttggaa acaactttta ggtggaatgt
    13741 ttgtgacgat tgcaatggta ggcttagatc aagatttaat gcagaaaaat ttaagttgca
    13801 agaacattgg cgaagcacaa aaaaacatgt ttgtattcac cggtatattt gtcattatca
    13861 atatcttttt tctaagtgtt ggtgcgttgc tttacatata tgcagagaaa aatggtattt
    13921 caattccaat ggtagatggt tcgccaagaa cggatttatt atttccagaa attgcgtttc
    13981 atcatttaag tttaattcca gccgtaatat ttttattggg attaacggca gcaacatttg
    14041 ctactaccga ttcggcttta accgctttga caacatcgtt ttgtgtagat tttttaggaa
    14101 tggataaagc agaaaatttc aaagaagcaa ctccaaaatc caaagtagaa atcgaaatta
    14161 gaaataaaaa attagtaaaa acccgtcatt tggtgcatat tggattttcg tttttgatgt
    14221 ttttagtaat cattattttc aattccttaa acgacaaatc agtagtaacg atgatattta
    14281 aagtagctag ttatacatat ggaccacttt taggattgta tgcatttgga ttattgatgc
    14341 aaacgaaaac cgttcatgat aaatttgtac cacttatttg catgatttct ccagcaattt
    14401 gtttttttat tgtaaagtat tctgaaacac ttttaggaaa atatgccatt gataacgaat
    14461 taatcattgt taatggtttt atcacctttt taggattact ttcaattagc aaacctgcaa
    14521 cagaaaatac acgtttttaa tattgattgg tagccaataa aactaaagta catgctacct
    14581 ctacttctat tccattggat tttagtaact gatagaactc tggattttca gttccaggat
    14641 taaaaataac gcgttttggc tttgtttcaa tgatgtaatt atagtaatct ctttgacgca
    14701 atggatttag atacaaagta accgtatcga tgttttttaa cggaatattt ttagtgtaaa
    14761 tttttatgcc tgctacttct cctgtgtttt gacctaaagc aattacagaa tggccattat
    14821 ccacaagttt tgttatggct ttaaatgcat aacgatctgg ttttgtacta gctcctaata
    14881 ccagtgtttt tttatttttc ataatcttta tataatttat caaagataat agttaggtaa
    14941 cataagtagg attattttaa ctgcttttta caatttatat caaatagagg atttatcttt
    15001 gcaaaaaaaa atggaagtct ttctaattct tttaggtgca ttattttcgg ttctaaatcc
    15061 aataggaact gttcctattt ttgtgagttt aacgcaagat tttacgaaaa aggaacgctc
    15121 aaatttgtcg ttacgaactg caattaatgt ttttataatc ttaattatat cgttttttat
    15181 tgggcaatat gtgcttacat ttttcggcat aaccattccg gctttgcgta ttgcgggtgg
    15241 aattatcatt gcaagttctg gtttttcatt attgaatgga aaattttcaa aaaatcgtgg
    15301 aattaataaa aaagtagagg aagacatcag tactcgaaat gcaatagctt taactccttt
    15361 agctatgcca atgcttgctg gtccgggttc catttcactt ttaattgctt ttcatcaaga
    15421 gcatgatgct acttttgaag tttttatgtc tagcttatcc attttagtag tgtcgatagt
    15481 aatttttttc attttaagaa gtgcacatta tttagccaaa atacttggtg catctggaat
    15541 tgttgctgta tcaagaatca ttgggttttt aaccatctcc attggaattc aatatattat
    15601 aacagctgta actgatattt ttgttaaaaa ttaatttctt ggtaaaaata tttccgccat
    15661 catacatctt gcacttccac caccacaagc ttcgatagta tccaaactag aatggattat
    15721 agaaacatgt gcttctaatt gacctatttg ttttttagtt aaacttttat aggcagattc
    15781 gctcatgata agaaaacgtt cgtcgttttt accttttagc tccaacatgt taccagcaaa
    15841 gttattcatt tggtcttcgg taatcaaaat gatttctttt tcatctcttc tcagcgaatc
    15901 caataccatt tttcgttctt ttttgtcatc aatacaatca gcacaaataa ctgcaaatgt
    15961 atcgcctaaa cacatcatta cattggtatg gtaaatcaat ttccgctctc cattaacggt
    16021 ttgataggct tcaaaaataa ctggtgaaag ttcaaaatct tcacaaaact caatcattaa
    16081 ttcttcatca gcacgcggtg aaagtgcaca ataggctttt ccgttttctc tatctaacaa
    16141 taaacttccg gttccttcaa gaaaaatcgc atcttcttcg gcagaagtat aatccatgat
    16201 ttcattgatt tgaaaacctt cgtcttcaag aatatctaat atatcttctc ttcgttctgc
    16261 acgtctattt tcggcaaaca ttggatataa aactacatcc ccattttgat gaaacgaaat
    16321 ccaattgttt ggaaaaatac tatcgggtgt attgggttct aaagtgtcat caactacaat
    16381 aacatttata ccaacttttt gaagtttagc tacaaaatca tcaaattctt tttgagcttt
    16441 ttcatttact gtttctggtg aaagcccatc taaaactttt tgatagtaat tgtttaccgc
    16501 agtttgttcg ttcatcctga acgctacagg acgaatcatt aatattgaat ttgtagtttg
    16561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttcgttcat cctgaacgct
    16681 acaggacgaa tcattaatat tgaatttgta gtttgattca ttgtttttta ttttgttatc
    16741 ggtttttagt tgtcggtttt aaattaagtt ttccagtttt ttttctacat aaacaataaa
    16801 gttaaatatt tttccaccca aattatcata actagatatt aactcatcaa tattatcaat
    16861 tgttccatga taaagaactg atattttgaa taaatgattt ttagtttcta agcaacttga
    16921 aagtgaataa accaaaaatt taatgaaatc agccttatat ctttttcttc cataaccttc
    16981 gacaatattt gacactatag agtctgaaga acgtcttatt taacttccta attcatacat
    17041 ttcgtattta ggtagtaaaa gcgatgcagg atgtacttta aaaaataaat ccaaactaat
    17101 tttataaata tctaaatctg cataatcgct catagtcggt tatcgcttat cggttgaaac
    17161 agtcaaataa actgtcaatt gtcaactttt tttaatccct tatcaatggt aatgttgaac
    17221 aacgcaaaag tccttcttgc ttggcaattt ctgcatacgg aatttcctca acgataaaac
    17281 cattattgcg taaccaagca tttaagcgag tgaaattctt ttcagaaacg actacgtttt
    17341 catctatcga gaacacgttt gaattcatgt tgtacatttc atcacgtgta atgtggaata
    17401 aattttcttt tccaaagagt tttaccaaat aaacataatc tgcttcttct cgaaagccac
    17461 ttttataaat tattcctttg tttttcccta ctggttgaaa acaacaatcg agatgcaatg
    17521 cattgtctct tgcttctaat tttgatttta ctaagtcaaa ttcttttaca attttgtttg
    17581 gaaataatgc tttgatatag tttactcctt ccatgttggt tcgcgctgtt atataatctt
    17641 tatagtcaga acctttgtag gttccaataa aaacataatc attccaaagc attacatcgc
    17701 caccttctat atgaacttct tctggcggac gaacaatttt ggatggatta atttggtcta
    17761 aaacatattg aatagcctcc agttctctgt ttctgtcagg aagaatattt gcttttacaa
    17821 agacatcgtc aattacaaaa cctatatcac gtgtgaagat ttgattgtaa ttttcaatca
    17881 tttctggacg aaaaactttg acatcatatt tttgaaaaac ttgattaaaa gcttccattt
    17941 ctttaatcat atcttcttca attggatacg ttccagcaag aatatgttct aaagattttg
    18001 gatcatacgc ttcttcaact gatggcgttg gaccattatt caaggctgaa ccaagcacaa
    18061 cggctcgtag tcgtgatgtt tcgtttttta catttaattg taacataaag ttgcttttgg
    18121 catttggtca aagataaaaa aactccaccg attggcgaag ctttgtttat aactgttttt
    18181 tacttgtata ttttaatttg tttctgaaaa taaaatacta aaaatcgttt tgcttttata
    18241 gaacttttta atgtaaatcc agctttttca attcctttaa tcgaagcaag attgttagcg
    18301 ttgacggtta taaaaacctc atctgtcttt ttttcaaaca attccttttt agcaatgtag
    18361 ttaatcacat agggataaat tgattttcct ttaaattcag aaattgtagc acattcacca
    18421 attactggtc cttttttgtg tatgagtttt aaaatatcta ttgatttgaa caagtaactt
    18481 tgatgcactt ttttcccttc aacttttata aaatattcaa ttttatttgg attactttta
    18541 tcttcttgaa tgtcaaaacc taaaaccgta catggtttgt attccgatgc gttcatagaa
    18601 taaaacaaca tatcgataga tataatcttg catcttttta aaattttttt tatcattttt
    18661 taaaaacaga taataggtta gcaatccttt ttttgtgttt ttttaatttt ttttcaacaa
    18721 tcgtataaaa tttaaaatag attggtttta gaatccaatg gtattgccca tcttgaatat
    18781 aataatgagt tgtgttaaag ctatttttaa attcaaaaac acctttagaa ccaccaagag
    18841 aaatattgta tccgtctaaa ccttcattta tggataattt aatggcttcc cattgtaaga
    18901 aatcaccagt tctcaaatcg ggggtttctt ttttagatcc acccaaaatg taggtgtaat
    18961 aatttccaga ttttactaat aaaatagctc cttttatttc attgctttta aatactgata
    19021 aaattttcaa caatcctttt tcgtttaatc cgaataaagt attctttatg tcttcccaat
    19081 ttctaatagt ataattggca ttggtagcat tttctaaaaa taatcgatat gcattttcaa
    19141 taccttcttg ggtatctaaa actttaaatt caagttcttt tctgtatgaa tttctaacat
    19201 ttcgtctaac agaaggtttt aagctcatta tcattttttc ttcatctaaa tgttttaaat
    19261 caacccagtt taacccactt gaagaataaa catatttaaa aagatgacct tttgctgctt
    19321 tttctaaaaa aggaattttt agagaattgg ttagtaaatg tacgttgtct atatctgaaa
    19381 atggaagatt tatttgagca tagcaacagt ttcttttttt cgctctatta taaactgact
    19441 caataatttc atttaatttt tcttcataat cactcgaaat aatgggtcca aatggtacaa
    19501 tataaaactt aaataccatt gcttttgcaa ttaccgcagc aaatccgcca ataattttat
    19561 cgttttctaa tgctaaacaa atctcaaatt caaaaccata cgattcaaat gatttattcc
    19621 agtcagacaa cattaaatga ctggctctat cttctgttac gatatagtca tcccattttt
    19681 ttaaccattt tggatcattt gtaaatagta ttttcatatt aataaagttc tctaaatggc
    19741 aaatcagaat gaagtaaatt tataatgttt cctgatttat cgacatctag tttaatggct
    19801 tcaaatttat ttaaaaaaag aactaattcg ttcaaagttg gattccaaat agttttttcg
    19861 tttatttttt tactcaaata cgaaaaattt tgagcaactg attcatcaat tgttgttggc
    19921 gttttaaaca attttccatt atgatgtttc attgagtcag aaaaataagt gtgggcaata
    19981 aaaatcccct tttcattgac taatttatca atgtttcttt tatttaaagc cttccgaaaa
    20041 tcaataattt ttacagtttg aaatatattg aatgtagaat tttgaaatcg atgcttgaaa
    20101 actaatggta aatatttggc taatttaaaa ggtttacttt tagtagaatt ccaaaataag
    20161 aataccttga ttattttaaa aaatactatc gtcaagttaa ttgtaaaaag acaaattttt
    20221 gaatactgtt taagtttaat tattcttttt actctgttaa aattataaat agtttcccga
    20281 tctccataaa aatgaaataa aataatttta attttaagtg ccattttatc tttaaatttc
    20341 aagtcttgaa ttccagcaaa ataacttttt aatgtaaaat cattagaatt tagttggttt
    20401 aaaacgccta acgttgctgt tccagaatcg atataattcc aaagagttgt tattttgttt
    20461 ttatttaaaa ttgctgcaaa tttctcttgt tcaaagccta aattttcaaa aaacgaaaaa
    20521 ttatacggtt gatacccgtg atctatccat gttggaacaa cctcaattgg tggttgaaat
    20581 gactcaaaat cctcaaaact ttctttgttg tgttttaaag attgtgaaag cgaatggtaa
    20641 cacaattcat gtccagcttt ttgccatttt aataattctg tactgttgtt ttcaaaggat
    20701 gcattttcgt tacgttttga aaaatgattt agaaaaaacc ctttggttat tttaatattg
    20761 ttctgctcaa aaaaaactct ttgcaattct aaattttcct gagtatcaaa gtcacaatga
    20821 tccgtaaagc aagcaatagc cgaaaaagga attggagaac gactaaattc gacagcattt
    20881 ttatccgaaa acaataaggc taattctacc gaaaagtcaa aatttgcagt aaccggttgc
    20941 actatttttt cgttcttttt tccagtatat tttgttattg aggcagcatg ttcaggatta
    21001 aaacgccacc gtattttgtt tttaaatttt ggattgaatt cccaaattcc gctttgaata
    21061 ttgggttgaa caaaaaaacc attgttgagt tttataattt ttggcgtaaa aacggaagca
    21121 attaattctc tgtcgcattt tatccattga taatcgtgat ttctaaataa gttgatttcg
    21181 tctttaaaag taatttcaat atcaaaagta ttgcattcag aaactttttg attttgtaat
    21241 ggaattaaaa gttttttttc aaaaaatgca acatctaaat gctcactttt atttgaaaaa
    21301 attaacttta aagattgtac cattagtagt aattgtagtt tttacaaata tattagaaat
    21361 tatagtataa aaataaaaag ctccgctgaa ttgcgaagct attattatag aaacatttat
    21421 aaatttacaa aaactatcct gcaaacgtaa ttatttcatt tccacgaaac ctaaccgttt
    21481 tgaaaggata attcattttt ttgaaaaatt cttctacttt aatataagta ggctggttgt
    21541 caatgatatg ataagaagca attgcaaatt taggttttaa tgtttttata gtctcaacac
    21601 atccttctat tgcttcaatt tctgcgcctt ctatatccat tttaatcaaa tccaatctgt
    21661 gaacattgtt gtttttgacc caattatcaa tagttacagc ctgtttttta acaatatttg
    21721 cgttctctga aaaccatttg gcagaagaag caaccgttcc tgattcttca aagtctagca
    21781 atgtatcttc attccacaat aacatatctt gtataattat gttatccttt aggtctgaat
    21841 ttagtttaat attattttct aaggatttaa tgttgtgttt gtctggttca aaacaaaata
    21901 ctttaccatt ttgaccaaca cattttgaaa aaaacataga aatatgacca acattggctc
    21961 ctgcatcaat tacaaaatct ccatttttag ctttataatt gtgctgataa tagccaaaat
    22021 caaccgctat tgagtataga gcttcgtttg tatataaaac tttattgtca aatgtagttt
    22081 tgtaaataac tttaccatta tctattttta tatcaaatga aattgcttta ttactaaata
    22141 gtttgtaata aaaaagctta atcttttcct tgctatttcc tttaggtatg aaaaagctaa
    22201 ttgtcttttt tatttttttc ttaagtttca ttattactaa ttttagccaa atataacaaa
    22261 aaaagcttcg ctaaattgcg aagcttttcc aaaaataaaa ggtaactaaa aaatttaatt
    22321 tttgattaat ttttttgaat aaacatctcc atcgtctgtc aatagattaa caatataaac
    22381 accatttgca taacgactta catcaaccct agccttgtct gtgagttcaa tattttgatc
    22441 aaaaaccatt ttcccatcta ttgttgtaat agaaatgttt ctaaattgat tgcctttcat
    22501 tatcaactca atagaactac tagaagggtt aggaactata agaaattcga tattcagttt
    22561 tgttacattt aattcttcaa gcaaaccttc ttctgttgaa cttgaatttg tatctgagtt
    22621 tctataaata taattcgacg aacagccttc aatataggct gaaaactgtg aacctgttac
    22681 agcttcaaaa cctgggttta attctacata atttcctgca tgatagacga caccattttg
    22741 aaatacagca tctccaacct caacaatatt agttgctttt atccaatcgg aacgttcttt
    22801 ttgagttgct gttgttgcat ctaaattggt aacattatct gctggtgcaa tcaaaattaa
    22861 tgtaggcaaa caacaatcaa taaacgtaac atccggacca acatttgccg aattgtcggt
    22921 catatcataa atataagttc cagccggaca agctacatta aatgttgcat ttacttcaaa
    22981 ttcaaaacta cccgatggat tggctccaaa agcacttggc aagaatgaaa aacaaaaagt
    23041 atctgatgtc aactgcgttg gcgcattgat tgtagttata gtatttccat tttgaataat
    23101 attcaacgat agtgtattta aagaagcatt tacgggtggt tgataggtac cacaaacgtt
    23161 tactggcact tctggagttg cattaggaca atttatattt gtaggatcta aactcaaagc
    23221 acctagttgt ggtgttgcac aaacaaatcc acataaatta tcaatataaa cggttccaaa
    23281 atgttgagat ggctgacaat cgcttaccga gaactcaatg gttcctggtt gatttaaaat
    23341 atcttgaaca tttaatcgag cacaaaccca tcctgtgtag agaattcttc tgctgttact
    23401 aacatttatt ctgttaaaca aacaattttc aggatcggca ataatacata tttgatctac
    23461 aacattattg ttttggtcta aaactctcac tctaaaataa ggttgaatgt cttgaccatg
    23521 tgccacttta ttatccatta ctaacgaaaa attaaaatca attgtaggtt gatttataga
    23581 aggcatatac cttgacatcg ttgtcaaatc ggacgaacca tagccttgag tattattcag
    23641 ttttatacat ttgctaccac tattggtgtt taaggttgga atattttgtc cgaaacttgc
    23701 caaaacagga tcgaattgct gatagtttgg tgctgtattg tcaatcaacg ttattgttga
    23761 attaaaatta tttaccgaag gtgttacaac attagtacct gttactacag ttggtgttcc
    23821 acaagattga aaaaaacttg aacccgaagc tggttgagga tgtgcatccg accaaaaaga
    23881 ataactggct gttccgtctt caaaatctcc attgacacag ctttgtctta atgaacttga
    23941 aattgggtta gcaatgtatt cttttttctt gtcaggaaat gttttaaaaa acaattttct
    24001 aagttcaaca acttttgctt tttcaatagc aacttctatt tctttgatgg aaagttcctc
    24061 aatttcatca ttcaaatgtt gaattgtttc tatttctatt tcattggtta atttatattt
    24121 accaatattt ttcacgataa aagcctgtac ttctttttca atattttgcg cttttgttat
    24181 tctgttttgc tccagtttta aataatcttc cacttgcgca aacgaagtaa ttgaacttaa
    24241 ccaaatgaaa caaaacagca gtagttttac tattttcata ctatttcttt ttttggatta
    24301 acaaactcat ttgggttttt aacgtttgaa tttcatcatt ttgtttttct aattttttat
    24361 tttgctcaat gatgtaaagt gttaattctt caattttttc catttgtttc gattgcattt
    24421 cagcgacatc tatcccattt tctgctaatt ctttggctga ttgaacgcca ggtaaatgct
    24481 tgtttatctt cacaaatttt tcaacttcat ttaatggtaa aagtttatag tctttttcaa
    24541 aaacataatc agcccaatta cttgtatttc gtaatgcaac ttttactttc tctgtcataa
    24601 ttccaccttc tacaaataat ttatagtttc cagaagttga tggaaaattt ggcgttgaac
    24661 caatatataa ttttccattg gatgtactat ttgttgtatc aaaccaaagg tttctattgt
    24721 tcaaatttac ttttctgttg ttgtttgttg tagttgcttg attgattgct ccatcattcg
    24781 catacaaact tgtatcaata gcgttaattt gaaatggcga tgttgtagat ccatctccag
    24841 aaatagtagt attcgttcca ggaattactg caaaagttgg tgttggaatg gttattgaat
    24901 ttccgccaga aatggataag gtattaccgt ttaaggttaa cgattgtgaa tcggtatttg
    24961 taaatgttgg aagtgttaca gagtttccgt ttaaaattga aagtacattt ccgtttaatg
    25021 ccaatgtttg ttggtcagta tcggttaatg ttggtaaagt aatagtgttt cctcccgata
    25081 tagaaattgt attaccgttt aaggttaacg attgtgaatc ggtatttgta aatgttggaa
    25141 gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttccgt ttaatgccaa
    25261 tgtttgttgg tcagtatcgg ttaatgttgg taaagtaaaa gaaccaccac caagtgataa
    25321 agaaactgta tttccattca aagtcaatac ttgaatttca ttagtcacac tttcatcaat
    25381 ctcggaaaaa tctggaattg gtaaggtaat tggtgcacta gaaacaccat taactgtagt
    25441 aaccaattga ttattggtat taaacgaatt tgaaattgta tttacaatag gtgcgtttgc
    25501 atttgagcca ttaacgaaac tattcattac atttccagtt gatgaaagtc cgtttgtaat
    25561 ggatgggttt gccatttttt gtagaataac atcaccattt tgattaacac tcaaaaattt
    25621 atcagaagtt tgcgaagaag taggaacata actactggtt aaattagtaa atctaagtcc
    25681 cgaatttccc gaagttccat gagtaatttc aagtttatta tttgggtttg aatttcctaa
    25741 accaacttta ccttcaacca gcaacccatt gacaggcgca acataactac ccgtatcaga
    25801 attgcttgga acgtagtttt ttccaattac tacaccaccg tttacatcta gcctattaat
    25861 tgggataata ttatttccca atcctaaacc catgtttcca attcgactaa taaaaattcg
    25921 ttgattccca gcaccatctg ctaaaataat tgatctatcc aaagagtttc ctgctattct
    25981 attactagaa acagtgttat ttaatctgac actgccaata aatacatttt ggttaccatt
    26041 tgttaagttt aatccatttt catttcctat aaaaatattt gaatttcccg agattaaact
    26101 tcttggtgtt tgataaccta aagcaatatt atcattacca tttactttta ctaaagtgcc
    26161 atatccaaca gcggtatttc tattaccaga tgtattagac cctaggcttc gatgaccaat
    26221 tgcaatatta ccgctaccgg ttgcattgtt ttctaaagtt cttgtaccca atgcaacatt
    26281 taaggtacct gaagtattta attttaatgc atcattacca aaagcacaat tgtaacctcc
    26341 tgtagtctgt attaatgtat tgaaaccaaa agcattatta ctatggctac tattattgat
    26401 tagtgaattg gtaccataac cagaattaaa atttccgcta ttatttgttc cagattcaac
    26461 tccaaaaaaa gtatttgctg tttgaccaat aactgtagaa aaatttaaaa ctaaaaaaga
    26521 tcctactaca aagtaatttt ttattttcat actataaaag attagggatt aaactcattt
    26581 tttttaataa ttatctataa tagtctgaat gactataaat aattattggt taaaaagttt
    26641 aatttttgta gtggtagatt gacaattcaa atatataaaa agagaacaat attatctatt
    26701 aaaagcatat tttttcgatt aaatacgctt ttttaattaa ataggaagat tatttcttat
    26761 aaaaaacact gaactttaaa aaacaagcta tttggcaaag gatttgttgt caataattaa
    26821 atttctattt gcaatatagt aagcgaataa aaatattaac attggtcgga atggaaacat
    26881 ataacgttgt tcagaatgaa acaaaaaaac ataaactaaa gccgaacccc aataaagtaa
    26941 tattggtata aaaaacctcc atttttttct tttaaacaat ttatacatcg ttattaaacc
    27001 aattattact aatatagaat tgattaaatt gatgtaaatg tgtatgccgt acttaaacca
    27061 aactggttta tgagatttca ttaatggagt aataatgaag gattgagatt gaaagaactt
    27121 taaaaaatat tgtctaagtg ttataaaggg atgattaaca atatcgtctt ttacccattt
    27181 aatataatat tgtgttctgt catatttatt agttaaacaa atagaatcta attcgtaacg
    27241 ttttcttaaa ttatttttat aatcattaga tgcgaaagaa tcgtttccat gttgtggcag
    27301 ccaattaaat ggttcgtctc taagttcaaa tcttgaccat aaaatttgat ttctaaacac
    27361 tttcttttta aactcattat catttttatc tagctttact tctgttagta tcaataaagg
    27421 aataattgta agtgtgagca agaaaattgt tttgttctta gtagtataac ttaaaaataa
    27481 aatatacaaa ccaaaagcaa tcaagaatgg aagagctgtt ggcttaattc catacaataa
    27541 tacaaaagca agtgctaaat aaacaaaatc acttgtttta gaagtttcag aaaaattaat
    27601 ttttacccaa aaataaatca ttacagaaac tgcaaaaaag gcgaaaattt ctccaataat
    27661 acccattgca taatagacat gtattggaaa caagcaaagt atcagtagtg taaaggcttt
    27721 tgattggttt gaaaacccta agattgtaaa ggctttaaat agataaaaga tggagagaca
    27781 aacaaaaaca cagctaaatg ctacaccact aaatagatat aaagcatcat tgtgaaaaga
    27841 atataccaat ccatagggaa ttaaataaca aaatagggtt agaaaccctt tattaatcat
    27901 tatttcacca tggaagccat ttttaataaa ttcaattaat tgaatagtac caaaatgaaa
    27961 atctccatcg ccaaaaggtt tgggtttaaa aggtaaaaaa taaaacaaca cacaaactag
    28021 agcggtaatg aatacagggt tttttgctaa aaaatcaatt attctcttct tcatttacaa
    28081 atttatcttt aataatatat ataggtcttt ttctagtttc atcatacgct cgccaaacat
    28141 actctccaat tatcccaagc attaccataa tcattccgcc aattatcaat aataaaatca
    28201 tgattggtgc ccatccatta aatggtgtgt tatttacaaa acggctataa attataatga
    28261 gggaatacaa aaatccaaca aatgaaaaac aaagtcctag aaaagacatt aatcggatgg
    28321 gaacataaga cgtattaagc aatccgtcta taaaatattt tattttttta gaaagtgtcc
    28381 attgcgattt gcctatggct ctttttattc ttctataagg tataaacgcc gtattaaaac
    28441 caagccataa aacatctcct tgaaaaaacc tgtttcgctc atcaatctga tttaaaacat
    28501 ttactgcttt tttgtccaat aataaaaagt caaatcctcc atttggcatt tttggattga
    28561 catatttcat taatttataa aatattttcg aggtattgtt tgcaataaaa ccgtcctctc
    28621 tatcagttcg atggcatatt acaatttcgt tgccttcttg ccatttttga accatttgct
    28681 cgataagttc tataggttct tgcaaatcgg cagacatact gattactaca tcgccagtta
    28741 cttcttttaa gccagcaatt acagcagcaa cttgtccaaa gtttcttgaa aatgacaaca
    28801 cttttacgtt agaatcgatt ttaaatagtt cttttaattc ttcaaaagaa ttatcgtctg
    28861 aaccatcgtt tacaaaaatt aattcgtatt caaatttatt ttttgttact aagtctttaa
    28921 ttttagtaaa tgttgggatt aaagaacctt cgtttctata aactggaatt acaaatgata
    28981 atttcattat aagaattaat tttttaacgt gtaaatataa aaaagcttct ctaatgagaa
    29041 gctttttatg taaaattttg caattatctt ttctctaacg gaacaaattc tcttaatgga
    29101 taacctgtat aaacttgtct aggtcttcct ataggttcgt tgtttaaacg catttctttc
    29161 cattgtgcta tccaacctgg taaacgacca atagcaaaca ttacggtaaa catatcggtt
    29221 ggaattccta atgctctata aataatacct gaataaaaat caacatttgg atataacttt
    29281 cttgatacaa aatactcatc tactaaggcc gcagcttcta attttttagc aatatctaga
    29341 attggatcat caacacctaa gctatttaag acctcatcag cagctttttt gataattctt
    29401 gctcttggat caaagttttt atatactcta tgaccaaatc ccattaaacg gaatgggtcg
    29461 tttttatctt tagcttttgc catatatttg tcagcatcac caccatccgc ttgaatggct
    29521 tctaacattt ctaaaaccgc ttgattggca ccgccgtgta atggacccca aagtgcagaa
    29581 actccagcag aaatagaagc aaacaaacca gcatgtgaag aacctactat tctaacggtt
    29641 gatgttgaac aattttgttc gtggtcggca tgtaaaataa atagtttgtc taatgcatca
    29701 attacggttt gattgtattt ataaggctcc gtaggtaatt caaacattag tcgcataaag
    29761 ttttctacat atccttttgt attatcatag ttatttaatg gaaatccttt tatttttcgg
    29821 taagtccaag ttgcaattac taaaaattta gccattgttt tacacacggc atcatacatt
    29881 tctttttcat tttctacgtt aacaactttg gggttgaaag ctgttaaagc acttgttaat
    29941 gatgccaaaa cgcccattgg atgtgctgtt tttggaaaac catcaacgat attcttcatt
    30001 tcttcattta ctaatgtata gttatgaata tcactttcaa attttgcaag ctgttctttg
    30061 gttggtaact ctccaaatat tactaaataa gacacttcaa gaaaatttgc actttcggct
    30121 aatgattcaa ttgaataccc tctgtagcga agaattcctt cttcaccatc taggaaagtg
    30181 atgtcacttt tacacgaacc tgagttttta tatccgggat ctaaagttat agcacctgtt
    30241 agtgctcgaa gtttttcaat atctatggca gtttcatttt cgcttcccgt tactaatgga
    30301 aattcgtatt ttttgccgtc aagttctaaa gtagcaattt ttgacatgtt tatatatgga
    30361 tttaatctta aaatgttatt aattccacaa aaataatgaa ttgtaaacga aataaaaaag
    30421 aattacaaca tcgttttagt taataatttg ataaatcttt tgacattttc ctttttgatt
    30481 tctttgggag gaatctacaa tggagttaaa tggataaaaa gcattattag acgatataaa
    30541 aaaccggttg taagcttcat taccccaact gaatatttca tttagctggc tggatactct
    30601 atttagctgt ctggatgctt tattactcca actggaaatt tcatttagct agttggatgc
    30661 tctatttagc tcgttataaa cgtcattact ccaactggat attttattta gcttgttgga
    30721 tgttctattt agctcactgg atgttttatt actcctattg ggtgttctcc ttctgttgta
    30781 gtatttaggt taaatagaga ctgtcaaaaa aactgtgtaa agtcattggt aatgtttaat
    30841 caactgcgta aagttacaac gttaaataaa ttagtacgag atgtaaatct atcggcttat
    30901 taagtgcttt ttgttacgtt tataatacaa aaaatcctgg gtaaatactc aggattttaa
    30961 aaataaataa ctaaccaatt tttactcttt taccaatttt tccactttta ttcctttttc
    31021 ggaaactatt tttaggaaat atattccttt tgattttgaa gttatgtcaa aagttgtttc
    31081 tgaattatta attatattgg tttgcaataa tcgtccttga acgtcgtaca actcgattgt
    31141 ttttatattg aagttgcttt tgatattgat tattgaactc gaaggatttg gataaatact
    31201 aatagaatta tcttgttcaa aacttggatt gttcaaggct tggaagacgg tatcggcttc
    31261 gttggtttct attgggaaat tgtaatcaaa atagatgttt gcttttttag ccactaaatc
    31321 accttgtatt aaatttggtt gacttcttat ttttaacaac acattgccat gaccaccaga
    31381 gtctaacatg atgttttgga aaataaattc aactatgttg ccacgaattc tagcttcaac
    31441 aggatgcgaa gtattcatca gttgcaaact gtttatatca aaatcggctg gattgacatc
    31501 aattttaact actatatttt cggctggtgc tgtacctgtg ttttcgaaac gtatattata
    31561 atgtaggtag ttcccaattt gtgttggtga tacaatatca ccttctagac aagtgatgtc
    31621 gtttggatca aaagaaccta caacagtttg gtttagtgta aaagcattgt caacccaatt
    31681 ttcatctgta gaaatgtcta taaaagaggt aaaaactaaa acatcaccaa tattgaccgc
    31741 tggtgtttca gttggtgcat ttacattcaa agt
//
LOCUS       DAOOBH010000013        30767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_1923_length_30767_cov_5.166710, whole genome shotgun sequence.
ACCESSION   DAOOBH010000013 DAOOBH010000000
VERSION     DAOOBH010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 30767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30767
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1923_length_30767_cov_5.166710"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PK218_02985"
     CDS             <1..451
                     /locus_tag="PK218_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97505.1"
                     /translation="SKASFSYERFLANDFSVGFNVNFNSSSKVKRDFEEGYVSNLPRY
                     EFNPYVRYALSKGKKTYYFAEVFGSYNVGDYKEIVYKVDANLNGYYKTETSTYTDFGA
                     GGALGYKMYFTDSFGMELLVGFGYNFANTYKSPDKISRVGLSLGYRF"
     gene            462..1052
                     /locus_tag="PK218_02990"
     CDS             462..1052
                     /locus_tag="PK218_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97506.1"
                     /translation="MKKLFYIVLFSSSFLFNSCDTNDDGFYNNVYLDAANLVTINTQP
                     TYTIGDYIYVTADFSRYLPDTANPATLLDVYQTTNGATQFAFSYVIEKKINDTDWEVV
                     TVTNNLLDINQGNATNGAYVYALCNYNATTENYQYNVGFPLLSAGNYRLSFGYNSSST
                     TKIELRSVSQPKNLVMNINSTVSGIDSSGYYIFTVN"
     gene            complement(1053..1718)
                     /gene="ung"
                     /locus_tag="PK218_02995"
     CDS             complement(1053..1718)
                     /gene="ung"
                     /locus_tag="PK218_02995"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPW97507.1"
                     /translation="MQFKLHPSWNDKIGSELEQPYFKKLATFVTNEYQSHICFPNTTE
                     VFAAFDYCSFEDCKVVVIGQDPYHGIGQANGLCFSVNDGVSFPPSLVNIFKEIEADLG
                     IPIPIWGNLERWAKQGVLLLNATLTVRKGEAGSHQKHGWETFTDAIIQKISDKKEGVV
                     FLLWGGFAQKKGAKIDRNKHLVLESGHPSPLSANRGFWFGNKHFSKTNDYLIQTNRKP
                     IEW"
     gene            1781..3952
                     /locus_tag="PK218_03000"
     CDS             1781..3952
                     /locus_tag="PK218_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPW97508.1"
                     /translation="MISITDKTLKDLEFTTVLQTISDRCNTEIGKQKALEIVPIKEKN
                     DLMDSLLQTSEYLSSFSNNNAIPNHGFDNLSNDLKFLNIEDSFLEVGSFRKIATLSET
                     VNTLLLYFKKFHDYYPKLNEKATKVEFTKYIVQKIDEVVDKYGEIKDNASPDLINIRR
                     DMNVVRGKVNQSFGQALNQCNSLGYLDEIKESFVENRRVLAVLAMYRKKVKGSILGSS
                     KTGSIAYIEPEATLRYSRELSNLEYEEREEITRILKKLSNEIRPYVELLWQYQDFLSD
                     IDVISAKAKYANKINGLLPTINENKRLFFREAFHPILYLNNLEKKEKTYPQTIELTNE
                     NRIIVISGPNAGGKTISLKTVGLLQLMLQCGILIPVHERSETFLFDRILTDIGDNQSI
                     ENHLSTYSYRLKNMNFFLKKCNAKTLFLIDEFGTGSDPELGGALAETFLEEFYHREAF
                     GIITTHYSNLKILANELPFASNANMLFDEKSLEPMYKLVLGQAGSSFTFEVAQKNGIP
                     YGLINRAKKKIEKGKVRFDKTIATLQKERSKLEKTSLNLKEEESKAREESKKMEGINV
                     KIQDKLERYQELYDANQRLIYIGQKIDDISEKYFNNKDKKVLIGEFLKVVEIENSKRK
                     KTTLKEKKAAEVIKNETIKEVTLKVEKIREKKIEEKKKKKEVEIKPTVVLKVGDRVRM
                     IDGKAIGTLDKIEKKKAVVNYGTFTSKVSIEQLEFVQATHK"
     gene            3971..4384
                     /locus_tag="PK218_03005"
     CDS             3971..4384
                     /locus_tag="PK218_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HPW97509.1"
                     /translation="MNELPIDKKIILFDGVCNLCNASVQLIIRKDKKDVFRFVALQSE
                     LGKKILNHIGVNTNSIDSILLYEPGNAYYYKAEAALKIATSLGGFYNLLVIFKIIPNV
                     VSNYFYDFVATNRYKWYGKKESCMMPTPELKAKFL"
     gene            4450..4587
                     /locus_tag="PK218_03010"
     CDS             4450..4587
                     /locus_tag="PK218_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97510.1"
                     /translation="MKSLILPLYSHGNGTIIMIGVFSLVVIGLVVAVLLMMGNSKNKK
                     D"
     gene            complement(4627..5682)
                     /locus_tag="PK218_03015"
     CDS             complement(4627..5682)
                     /locus_tag="PK218_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97511.1"
                     /translation="MKKILLLMFAFSVSLANAQVLQSEDFNTLNNGDVGTDITGVTPG
                     QGEWLTFSTNGAAPTTSDNAGNSNFVIVDNGNEVTNGLKIIGANGNKGSRYMWKDGLG
                     TAWDGRDLGNDIIEVEFDLYTGAATTSTAQVGVRLFGTDDSVTPSVTRTLNGFVYTMN
                     TRVLTGVAYLNNAGTYNTFLITLQTGGLILDADTWYRIGFAYDSNTGETIWKTPTVYT
                     GLPAANWAGPFIVDEVDFITTTPTTNAAVSEIIYDNLTVKATPEEALLNVSEVIAEGS
                     FSVYPNPAKNVLNISNTINAVVNNVVLTDLNGRVVKSQNLNTVEGQVNISDLATGIYM
                     MNVNTDKGSITKKIIKE"
     gene            complement(5753..7005)
                     /locus_tag="PK218_03020"
                     /pseudo
     CDS             complement(5753..7005)
                     /locus_tag="PK218_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
     gene            complement(7006..8298)
                     /locus_tag="PK218_03025"
     CDS             complement(7006..8298)
                     /locus_tag="PK218_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPW97512.1"
                     /translation="MRGFKLKMHWKILIGMILGLLFGLLMKSIEQKGLIIDWIKPFGT
                     IFINLLKMIAVPLILVSLINGLADLKDISKLSKLGGRTISLYLSTTVIAVTIGLILAN
                     VINPGSFINEASRESLLQNFASDAAQKIEIASQTKNNGPLQPLIDIVPSNVFHAMTDN
                     ASMLQVILFAILIGIGLISIKRKQAKPVVKFFKGLNEVILKMIDFIMLAAPIGVFALM
                     AALIVEIPDFTTLQALLVYALTVVLGLGLMMFIVYPTLLYSFAKVKPKRFFNAIAPAQ
                     LLAFSTSSSAATLPVTMECVTENLGVDDEVASFVLPLGATVNMDGTSLYQAVAAVFIA
                     QAMGIHLDLSAQLMIVLTASLASIGSAAVPSAGMVMLVIVLGQAGIPEAGLALIFAID
                     RPLDMCRTAVNVTGDSAVSGIVASSIGKLHTKKSNLFD"
     gene            complement(8299..9360)
                     /gene="aroC"
                     /locus_tag="PK218_03030"
     CDS             complement(8299..9360)
                     /gene="aroC"
                     /locus_tag="PK218_03030"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPW97513.1"
                     /translation="MAGNSYGTLFKLTTFGESHGEALGGIIDGCPPGIVLDFEAIQKE
                     MHRRKPGQSTLVSQRKEEDEVQFLSGIFEGKTTGTPIGFIIQNTNQKSDDYSHIKDSY
                     RPSHADYVYEKKYGIRDYRGGGRSSARETASRVAAGAIAKQVISNIKINAFVSSVGEI
                     FIDKPYQSLDFSVTETNAVRCPDYDTAQKMEEYIKEIKKQGDTVGGTITCVIQNVPVG
                     LGEPVFDKLHAELGKAMLSINAVHGFEYGSGFCGAKMKGSEHNDLYNEDGTTRTNLSG
                     GIQGGISNGMDIYFRVAFKPVATLLQKQEVLTNKATIEEQQGKGRHDACVVPRAVPIV
                     EAMAALVLTDFYLINKIYS"
     gene            9532..10365
                     /locus_tag="PK218_03035"
     CDS             9532..10365
                     /locus_tag="PK218_03035"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPW97514.1"
                     /translation="MKKRKLDLVVISDTHLGTYGAHAKELYHYLNSIKPKTLVLNGDI
                     IDIWQFRKSYFPKSHLKVIKKIIDLASKGTKVYYLTGNHDEMLRKFSPATLGNFILAD
                     KVVLDLDYKKAWVFHGDVFDASVQHSKWIAKLGGLGYDYLIVLNRMVNWCLKKMRKEP
                     YSFSKKIKASVKKAVKHISDFENTATELAIENKYDYVICGHIHEPKITRQVRKDGNTI
                     YLNSGDWVENLTALEYKNKRWKLYRYEDHTEKEDEDLFELDDLIKHQLIASMVMKQPL
                     M"
     gene            10487..10984
                     /locus_tag="PK218_03040"
     CDS             10487..10984
                     /locus_tag="PK218_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97515.1"
                     /translation="MKKIILSAFMLIGLAFATQAQDISKNALGLRLGDNDGFGGEVSY
                     QRGLGDNNRLELDLGWRNSKNYDAIKLVGLYQWVWNIDQGFNWYAGVGAGVGSWSYNK
                     SGQSASGTFILAAGDIGIEYNFDIPLQLSLDLRPEFYLNNNGNNQFRENNFGPDFGLG
                     IRYRF"
     gene            complement(11030..11458)
                     /locus_tag="PK218_03045"
     CDS             complement(11030..11458)
                     /locus_tag="PK218_03045"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HPW97516.1"
                     /translation="MMKRICLLLIGLTLFSCSTSKKEMVKKPLYEVLVTKNDGGANIK
                     FYEIISEPKEINMLMGDNDLRKLIKKDDILSCNYIIINLGPLPNGNYTTTLEKVEETP
                     TNIILKIKELKIKEANVADENTTYPYSVVKINSKKPIIFK"
     gene            complement(11455..12603)
                     /gene="bshA"
                     /locus_tag="PK218_03050"
     CDS             complement(11455..12603)
                     /gene="bshA"
                     /locus_tag="PK218_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HPW97517.1"
                     /translation="MKIAIVCYPTFGGSGVVATELGLELAHRGHEIHFITYRQPVRLA
                     LLNPNVHYHEVNVPEYPLFHYQPYELALSSKLVDMVKLHKIELMHVHYAIPHAYAGYM
                     AKQMLKDEGINIPMITTLHGTDITLVGNHPFYKPAVNFSINKSDIVTSVSQSLKEDTY
                     ALFDTQKEIHVIPNFIELDKHRNEQQISCQRSVMASKDEKIITHISNFRKVKRIPDVI
                     QIFNKIQQKIPAKLMMVGDGPEKEKAEALCQKLGIQDKVIFFGNSNEIDKILSYSDLF
                     LLPSETESFGLAALEAMAWSVPVVSSNSGGLPEVNFEGVSGYLSDVGNVDDMAENALK
                     ILENETILNEFKANALSVAKQFDIKNILPLYEDLYRLAIQNSKSIVQS"
     gene            12940..14364
                     /locus_tag="PK218_03055"
     CDS             12940..14364
                     /locus_tag="PK218_03055"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HPW97518.1"
                     /translation="MEECISVFDMLKIGVGPSSSHTLGPWRAAERFLRELHEEKILDT
                     ILTIKVDLYGSLSLTGKGHATDLAVMLGLSGQDPVTIPVQNIDTIIKKIQKTKQLLLG
                     NQHTIAFNPDTDIVFNREFLPFHANGMTFTATTKLGNYTNTYYSIGGGFVVVEERLNA
                     KKKMEFKCAFPYPIDNANMLLKYCTDENKSISEIVMINEKSMRSEKEINEQLMTIWHT
                     MLECMYIGCHSEGILPGGLNVRRRAFDMHQGLIGLANYSNPQEWLESIRKTEVKFRQI
                     LKWVSCFALAVNEVNAALGRVVTAPTNGSAGVIPAVLMYYLVIENHNAGEKEIKQFLM
                     VAGEIGSLFKQGSTISAAMGGCQAEIGVSSAMAAGALCEVMGGTPDQVLMAAEIAMEH
                     HLGLTCDPIGGLVQIPCIERNTMGAIKAINAAELAFETDAKNAKVPLDKVINTMWQTA
                     KDMNSKYKETSEGGLAIAVNMADC"
     gene            complement(14376..15677)
                     /locus_tag="PK218_03060"
     CDS             complement(14376..15677)
                     /locus_tag="PK218_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW97519.1"
                     /translation="MSKLTLIIRREFLAKVRNKSFIVMTFLSPLLFVGIGVFVGFLST
                     MKPEVKTIAVHDETGNYFKLLKNSDEYYYTDFSLIDVKTLKDSIISEAYEGLLLIPKK
                     ANPDSLAKSIDYISNDSPDFDIISDLEKTIGQSITQQKYASNGLDTLKIKELKSMVAI
                     NIKKASGEKSIQGLNWIKTIAGGAFGYLIMMFIVLYGNMVMRSVIEEKTSRIIEVIIS
                     SVKPFQLMMGKIIGTSLAGILQFLIWALLGLSAMFILSSVFGVQVGATGAQSQQAMEM
                     ASKNMSNVQLYFQEIWNLPIATLLVSFMLYFIGGYFLYSSFYAAIGAAVDNETDSQQF
                     LLPIILPLILGVYIGFFTVINDPHGTIATVFSMIPLTSPIVMMMRIPFGVPLWQIVIS
                     LVLLFGTFILVVWFASKIYRVGILMYGKKPSWKELYKWSKF"
     gene            complement(15753..16673)
                     /locus_tag="PK218_03065"
     CDS             complement(15753..16673)
                     /locus_tag="PK218_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW97520.1"
                     /translation="MNNILEVKNVVKTYGDFTALNEVSLTIPKGSIYGLLGPNGAGKT
                     SLIRIINQITLPDSGEILLDGEKLNLNHIQNIGYMPEERGLYKTMKVGEQCLYLAQLK
                     GLSKLEATKELKYWFERLEIQGWWNKKIQELSKGMAQKIQFVVTVLHKPKLLILDEPF
                     SGFDPVNANLIKDEIIELNKQGTSVIFSTHRMESVEEMCDHIALIHKSNKLIEGKLSD
                     VKKEYRTNTYEVGILTDNVEGLMFDLTQKFTLSQTDFKSLNDELKLAINLGNATPNEL
                     LNTLVQRGQVTHFVEKIPSVNDIFIKTVTE"
     gene            complement(16910..18031)
                     /gene="dnaJ"
                     /locus_tag="PK218_03070"
     CDS             complement(16910..18031)
                     /gene="dnaJ"
                     /locus_tag="PK218_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPW97521.1"
                     /translation="MKKDFYEILGVTKSASTEEIKKAYRKKAIEFHPDKNPGNKEAEE
                     KFKLAAEAYEVLSDADKKAKYDQYGHAAFDGAGGFGGGHHMNMDDIFSQFGDIFGGAF
                     GGSFGGGFGGNSGGQRRVKGSNLRIKVKLTLEEIANGVEKKVKVKRKIQAPGVKYKTC
                     GTCNGQGQVLKVTNTILGRMQSATTCHVCGGSGQTIESKPNNADAHGMILEDETVSIK
                     IPAGVVDGMQLKVSGKGNDAPGNGIAGDLIVAIEELEHEFLKREGENLHYDLYISIAE
                     AVLGVSKEIEAVNGKVRIKLDEGIQSGKILRLKGKGIPSLNSYGNGDLLVHINVWTPK
                     TLNKEQRQFFEKSLTDDNFLPHPEKSDKSFFEKVKDMFS"
     gene            complement(18085..18654)
                     /locus_tag="PK218_03075"
     CDS             complement(18085..18654)
                     /locus_tag="PK218_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPW97522.1"
                     /translation="MKFKDVFKTKKAMNTDNTEKEPIENEQENSFNGVEAAEELTVEQ
                     QLQEELANEKDKFLRLFAEFENYKKRTTKERFDLYKTANQEVLQAMLPVLDDFDRAMI
                     QISKSEDETLLKGVELIHEKLKSTLASKGLEIVELKAGDVFNADFAEAITQIAAPSKE
                     LKGKIVDVVEKGYKLGDKIIRFPKVVLGA"
     gene            complement(18907..19809)
                     /locus_tag="PK218_03080"
     CDS             complement(18907..19809)
                     /locus_tag="PK218_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HPW97523.1"
                     /translation="MKLLITGATGLIGTALVKQLHKNGHTINYLTTSKNKLKSEPNYR
                     GFYWNPEQNEIDTDCLKGVEAIIHLAGASIAKRWTKKYKKEIFSSRTKTADLLFSTLK
                     NTNHNVSHFISASGTAIYPESFEKVHDENTTATANDFLSEVVKVWEKAANQFEILGIK
                     VSKIRTGVVYAKSGGAFQEIVKPIKFCVGAIMGNGKQMQSWIHIDDLVNLYCFIAEQQ
                     LEGIYNAIAPETISNETQTKSIAKYLNKPLFLPNIPQFLMKIVLGQMSILLFTSKNLS
                     SEKIRKKGFSFIYPNFNAALKELL"
     gene            complement(19883..20338)
                     /locus_tag="PK218_03085"
     CDS             complement(19883..20338)
                     /locus_tag="PK218_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HPW97524.1"
                     /translation="MPITPIKLNTFLFFKLPSAFWSGVRLKSISKDNCVVTVKHRWFN
                     QNPFNSMYFAVQAMAAELTTGALVMMKIRESNQKISMLVANNKGNYSKKATGRITFTC
                     NDGHLIEEAIKKTIATREGQTIWMKSIGVNQKGEQVSEMDFEWSVKIKV"
     gene            20537..20992
                     /locus_tag="PK218_03090"
     CDS             20537..20992
                     /locus_tag="PK218_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPW97525.1"
                     /translation="MTSTNDRNTATFIHLSTLSQYIIPFGNYIIPIIIWSSKKNESQF
                     IDVNGKNAINFQLSMFLYTMITLVVAIPVFIYSIFNNTTIKNIHDGRDFIVDHLNAGN
                     ISGIIILAIITVLLFCFLKVIEFILIIYASAKASNGEAYKYPLTINFIK"
     gene            21072..21404
                     /locus_tag="PK218_03095"
     CDS             21072..21404
                     /locus_tag="PK218_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPW97526.1"
                     /translation="MNIENTKAQMRKGVLEFCILSIIKEKEAYTSDILETLKDAKLLV
                     VEGTVYPLLTRLKNDGLLSYRWEESTSGPPRKYYGLTELGKTFLNELNGTWTELSDAV
                     NIITTKNN"
     gene            21407..23137
                     /locus_tag="PK218_03100"
     CDS             21407..23137
                     /locus_tag="PK218_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPW97527.1"
                     /translation="MNKTVNINIGGLIFHIDEDAYQKLSRYFEAIKRSLSNSSGQDEI
                     MKDIEMRVAELLTARQQSDKHVINNKDVEEVIAVMGQPEDYRIDDDTEQPKTETYYTS
                     SSRKKLYRDKDRGIIAGVCTGLGHYSGVDAVWFKILFLIFVFAGFGTGIIAYFILWIA
                     VPKAITTSEKLEMTGQPVTISSIEKKVREEFDSVSEKIKNADYDKLGSQVKYGAERVG
                     NGVGDVLIKIFGAFAKVLGALILVFSSMSLVGLLIGMFTVGSTSFINTPWQRYIDAVN
                     YTDVPLWAFSLLSFLAIGIPLFFFLLLGLKLMVNNMKSIGSPIKYSFLAIWIFAVGAL
                     VFIGIKQSTERAFDGKMVTKEMINIQSNDTLRIKFVNNDFFSKDINNHEDFLFTQDSL
                     KNEVIYSNEVKFIIKKTDKPQPYLQIERLAEGKSFNEANLRAEKIKYSFKINGNQLLL
                     DNYLLTEMASKYRNQKVELYLYLPEGIVYYPDENVKYFLEGSNSDFDYYYGPEGYKYK
                     VKNTDLICLNCPDDENNDVKTEDINASDENDSIKTVTVKINGKVVSETKSDKKGSLKI
                     NKDGVIIKTN"
     gene            23140..23880
                     /locus_tag="PK218_03105"
     CDS             23140..23880
                     /locus_tag="PK218_03105"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPW97528.1"
                     /translation="MIKFVMFCVKAFVATLVGLLVTSCNGKIDLGNSITGSGKVVKEV
                     RNLDSFDKVTVCCGLECEIIQAPNFKVTVEADDNLIKDIKTSVINGTLKISTDYGNYV
                     NVTSKKIIVEMPNVKSLETTSGSSLKTIKTIRENSLSLKTSSGSEMNVETESDTIRLE
                     STSGSEQKIKGKALVLFTSSSSGSSIEAEGLLANEISSQSTSGSSTEVNPIVSLKAKA
                     SSGSSIDYKNKPKSISKEESSGGSVSRN"
     gene            23957..24790
                     /locus_tag="PK218_03110"
     CDS             23957..24790
                     /locus_tag="PK218_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HPW97529.1"
                     /translation="MKKSLSLLLLLLVTFSFAQRKEKVKGSKIVKMEQKQVESFTSLE
                     VEDNLEVFLVKGNECGLEIEADDNLIEFIDYKSAGNNLRISTTRDMNSFKKLSVRVTY
                     TSDLNLIIAKHETNVTALSDVDLANVTFKSYDYAKLFLNAKTKDFTLMANDKSKIELN
                     LKSEKTTIDISKNAELKALISSKDLGIDMYQKAEAKIEGDVINFKLRADNNSNFDGAK
                     LTTTNTQIFADAYSSAKINVKTNVIIDAAGKSEIQLFGEPKIEIKRFTDSATLEKKPI
                     K"
     gene            complement(24837..25784)
                     /gene="trxB"
                     /locus_tag="PK218_03115"
     CDS             complement(24837..25784)
                     /gene="trxB"
                     /locus_tag="PK218_03115"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPW97530.1"
                     /translation="MSDTIEKVKCLIIGSGPAGYTAAIYAARANMNPVLYQGTQPGGQ
                     LTTTNEVENFPGYVDGVTGPEMMIQLQAQAQRFGTDVRDGWATKVDFSGKTHKVWIND
                     SKEIHAETVIISTGASAKYLGLPSEQHYLQTGGGVSACAICDGFFYRNQEVVIVGAGD
                     SACEEAHYLSKLCKKVTMLVRSDKFRASKIMEERVKKTENITILMNHDTVEVIGDGQV
                     VTSVKALNKTTNEEIDIPATGFFVAIGHKPNTDIFKDFLELDQTGYIKNIPGTSKTNV
                     AGVFVGGDAADHVYRQAVTAAGTGCMAALDAERYLASMD"
     gene            25936..26010
                     /locus_tag="PK218_03120"
     tRNA            25936..26010
                     /locus_tag="PK218_03120"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25971..25973,aa:Pro,seq:tgg)
     gene            26128..26736
                     /gene="udk"
                     /locus_tag="PK218_03125"
     CDS             26128..26736
                     /gene="udk"
                     /locus_tag="PK218_03125"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPW97531.1"
                     /translation="MLIIGIAGGTGSGKTTVVHQIMNELPQTEVGIISQDSYYKENNT
                     LSFEERALINFDHPRAIDFELLVQHLKELKEGNTIEQPIYSFITHNRTDDSVITHPRK
                     VMIVEGILILSNPELRDLFDVKIFVHADSDERLIRRLKRDIAERGRDMEEVLNRYQTT
                     LKPMHQQFIEPTKAFADIIIPNDKYNTVAIDVVRAVINQRIT"
     gene            26758..27111
                     /locus_tag="PK218_03130"
     CDS             26758..27111
                     /locus_tag="PK218_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPW97532.1"
                     /translation="MSYFTNIIDKYPILKILSNRFVIVLIFFAIWMIFLDNTSYFDHR
                     VLNKELEELEVNKKYYQDEIKRDEEDIKRLKNPDQIEKYAREKYYMKRDSEDIYIIEY
                     EGDTIHEEQSKTKSL"
     gene            27121..28479
                     /locus_tag="PK218_03135"
     CDS             27121..28479
                     /locus_tag="PK218_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HPW97533.1"
                     /translation="MENKLFDSFETISTKQWKQKIQYELKGADYNDTLVWESLEGIKV
                     KPFYHSDEMENKFSFNEAKPFSILQSIYVHDVAKSNKRTLETLNRGAESIRFTIENET
                     ISVNELMENLPKGNTTYFFYLPFLSISFINKLNDFSLDNGYDFKLQIDPIYQLCKDGN
                     WFDNLYKDFDILNQIACKAKSSFITIDSSIYQNAGANIVQQLAYTLAHVNEYFNRISK
                     INQPITIEISVGSNYFFEIAKLRALRLLFNTLTKEYNHNLECIIIATPTKRNKTLYDY
                     NVNLLRTTTECMSAILGNANFISNLPYDTLYHKDNEFGDRIARNQLLILKHESYFDEV
                     TNAADGSYYIEELTAQLAEKALLLFKEIEANDGLITQLIAGTIQKKISESAAKEQEWF
                     DSGKEVLLGTNKYPNKNDKMKNDLELYPFVKQNPRKTLIVPIIEKRLAEKLEQERLAQ
                     EQ"
     gene            28484..30616
                     /gene="scpA"
                     /locus_tag="PK218_03140"
     CDS             28484..30616
                     /gene="scpA"
                     /locus_tag="PK218_03140"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPW97534.1"
                     /translation="MRKNIQHITLENSKKEFPNFNNSELRTFQTAEGIELKPTYSEDD
                     IKELEHLGFGAGFAPNLRGPYTTMFVRRPWTIRQYAGFSTAEESNAFYRRNLAAGQKG
                     LSVAFDLATHRGYDSDHERVVGDVGKAGVAIDSVEDMKVLFDQIPLDEMSVSMTMNGA
                     VLPIMAFYIVAAEEQGVAPHLLSGTIQNDILKEFMVRNTYIYPPTPSMKIIADIFEYT
                     SKNMPKFNSISISGYHMQEAGATADIELAYTLADGLEYIRTGLSTGMNIDEFAPRLSF
                     FWAIGMNHFMEIAKMRAGRMLWAKLLKQFDPKDDKSLALRTHCQTSGWSLTEQDPFNN
                     VARTTIEAAAAAFGGTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETKICKTVDP
                     WAGSYYVESLTAQIAEKAWVLIEEVEELGGMTKAIEAGIPKLRIEEAAARKQARIDSG
                     QDIIVGVNKYRLDKEDPLHILEVDNQTVRKQQIEQLEHIKVTRDTAKVKASLEKLTDC
                     AKNQNDNLLSLAVEAARNRATLGEISDALESVYGRYKAQIRSFSGVYSKEIKNDESFE
                     KAKQLADAFAKKEGRRPRIMIAKMGQDGHDRGAKVVATGYADVGFDVDIGPLFQTPHE
                     AAKQAVENDVHILGVSSLAAGHKTLVPQVIEELKKYGREDIMVIVGGVIPSQDYQYLF
                     DAGAVAVFGPGTKISDAAISILEVLLET"
BASE COUNT        10553 a         5198 c         4953 g        10063 t
ORIGIN      
        1 ttcaaaagca agttttagtt atgaacgttt tttagccaat gatttttctg ttggatttaa
       61 tgtgaatttc aatagcagct ccaaagtaaa aagagatttt gaagaaggat atgtgagcaa
      121 tttaccaaga tatgagttca atccctatgt gcgatatgca ttatcaaaag ggaaaaaaac
      181 ttattatttt gccgaagttt ttggttccta caatgttggt gattataaag aaattgttta
      241 caaagtagat gctaacttga atggatatta taaaacagaa acatctactt atactgattt
      301 tggagctggt ggtgctttag gatataaaat gtattttact gatagttttg gaatggaact
      361 tttagtaggt tttggttata acttcgccaa cacctataaa agtcctgata aaatatcaag
      421 agttgggctt agtttaggtt accgttttta atataaaatt tatgaaaaag ttattctata
      481 tcgttctttt tagcagttca tttcttttca acagttgtga taccaacgat gatggttttt
      541 acaacaacgt ctatttggac gctgcaaatt tagtaactat taatacgcaa ccaacatata
      601 caattggtga ttatatatat gtaactgctg acttttcaag atatttgcca gacactgcca
      661 atcctgctac tttgttggat gtatatcaaa ctactaatgg tgctacacag tttgcctttt
      721 cttatgtaat tgagaaaaaa ataaacgata ccgattggga agtagttaca gttactaata
      781 atttattaga cattaaccaa ggaaacgcca ccaatggcgc atatgtttat gccttatgta
      841 actataacgc aactactgaa aactatcaat acaatgttgg ttttccatta cttagcgctg
      901 gaaattaccg cttgagcttt ggttacaata gttcttcaac tactaagatt gaattacgtt
      961 cagtaagtca gccaaaaaat ttagtgatga acattaattc cactgtttca ggaattgata
     1021 gtagtggtta ttatattttt acagtaaatt aattaccact caataggttt tctgttggtt
     1081 tgtatcaaat aatcgtttgt tttactaaaa tgcttgtttc caaaccaaaa tccacggttg
     1141 gcactcaacg gagatggatg tcccgattct aaaactaaat gtttatttcg gtcaatttta
     1201 gcaccttttt tttgtgcaaa tccaccccaa agcagaaaga caacaccttc ttttttatct
     1261 gatatttttt ggataatggc atcggtaaag gtttcccaac catgtttttg atggcttcct
     1321 gcttctcctt ttctaaccgt taatgtggca tttagcaata aaacaccttg tttagcccat
     1381 cgttcaagat ttccccaaat tgggattgga atacctaaat cagcctctat ttctttgaaa
     1441 atattcacca aagacggcgg aaaagaaaca ccatcattca ccgaaaaaca caagccattc
     1501 gcttgaccaa tgccatgata cggatcttga ccaatgacaa ccaccttgca atcttcaaac
     1561 gaacaataat caaaagcggc aaaaacttcg gttgtattgg gaaaacaaat gtgtgattgg
     1621 tattcgttag ttacaaaagt ggctaatttt ttgaaatacg gttgctccaa ttcacttcca
     1681 attttatcgt tccaagaagg atgtaatttg aattgcattt tagttttttt acaaatataa
     1741 atagtttaaa acattgtaac tttgtgtcaa gatacaattt atgatttcaa taacggacaa
     1801 aacacttaaa gacttagaat tcacaacagt tttacagact atttccgata gatgcaacac
     1861 tgaaattgga aagcaaaagg cattagaaat agtacctatt aaagaaaaaa acgatttaat
     1921 ggattcgtta ctccaaactt cggaatatct ttcgtcgttt agtaataaca acgctattcc
     1981 aaatcatggc tttgataatc tatccaatga tttaaagttt ttaaacattg aagatagttt
     2041 tctagaagtt ggtagtttta gaaaaatagc cacactttcg gaaactgtga atacattgtt
     2101 gttgtatttt aaaaagtttc atgattacta tccaaaatta aacgaaaaag ctaccaaagt
     2161 tgaatttaca aaatacattg ttcaaaaaat agatgaagtt gtagataaat atggcgaaat
     2221 aaaagacaat gcatcacccg atttaatcaa tatacgtcgc gatatgaatg ttgttagagg
     2281 caaagtaaat caaagtttcg gacaagcctt aaatcagtgc aattcattgg gatatttaga
     2341 tgaaattaaa gaaagttttg tagaaaatcg tagagtttta gcggttttag ccatgtatcg
     2401 aaagaaagta aaaggttcta ttttaggaag ttctaaaaca ggaagcattg cttacatcga
     2461 accagaagct acattacgct attcaagaga attgagcaac ttggaatatg aagaacgaga
     2521 agaaataaca agaattttaa agaaactttc taacgaaata agaccatatg tggaattgct
     2581 ttggcaatat caagacttct taagtgatat tgatgtcatt tcagcaaaag caaaatacgc
     2641 taataaaata aatggtttac taccaactat aaatgagaac aaaagacttt tttttagaga
     2701 agcttttcat cctattttgt atttaaacaa tcttgaaaaa aaagaaaaaa catatccaca
     2761 aaccattgaa ttaactaatg aaaacaggat tattgtaatt tctggtccaa atgctggcgg
     2821 aaaaacaata tcgctaaaaa cagttggttt acttcaatta atgcttcaat gcggtatatt
     2881 aattccagtt catgaacgta gtgaaacctt tcttttcgat aggattctaa cggatattgg
     2941 cgacaatcaa tcgatagaaa atcatttgag cacctacagt tatcgcttga aaaacatgaa
     3001 tttcttttta aaaaaatgca acgcaaaaac gttattttta atagacgaat ttggtactgg
     3061 ttccgatcct gaacttggtg gtgctttagc agaaacattc ttagaagaat tttatcatcg
     3121 ggaagctttt ggaattatta ccacacatta ttcaaattta aaaatccttg ccaacgaatt
     3181 gccttttgca tccaatgcta atatgctttt tgatgaaaaa tcattggaac caatgtataa
     3241 attagttctt ggtcaagctg gtagttcgtt tacttttgag gttgcccaaa aaaatggtat
     3301 tccttatgga ttaatcaatc gtgccaaaaa gaaaatagaa aaagggaaag tgcgatttga
     3361 taaaaccatt gctactttac aaaaagaacg ttcaaaattg gaaaaaactt ctttaaatct
     3421 taaagaagaa gaaagcaaag cgcgtgaaga gagcaaaaaa atggaaggca tcaatgttaa
     3481 gattcaagac aagttagaac gttatcaaga gttgtatgat gccaatcagc gtttgattta
     3541 tataggacaa aaaattgatg atatttcaga gaaatacttt aataataaag acaaaaaagt
     3601 attgattgga gagtttctaa aagtggtaga aattgaaaac tctaaacgaa aaaagacaac
     3661 tttaaaagag aagaaagctg ctgaagttat aaaaaacgaa accatcaaag aagtaacctt
     3721 aaaagttgaa aagattaggg aaaaaaagat agaggaaaag aaaaagaaaa aagaagtaga
     3781 aattaaacct actgtcgttt taaaagttgg cgatagagtt cgaatgattg atggaaaagc
     3841 tattggaact ttggacaaaa ttgagaaaaa aaaagccgta gttaactatg gaacatttac
     3901 ttcaaaagta agtatagaac aactggaatt tgttcaagca actcataaat aaatttagat
     3961 aatcatggag attaatgaat tgcctattga taaaaaaatc attctttttg atggtgtatg
     4021 caatttatgc aacgcttctg ttcaactgat aatcagaaaa gataaaaaag atgtttttcg
     4081 atttgttgct ttgcaatctg aattaggaaa gaaaatttta aatcatattg gtgtgaatac
     4141 aaattctatt gatagcattt tgctttatga gccaggaaat gcttattatt ataaagccga
     4201 agctgcttta aaaattgcaa catcattggg tggcttttat aatttattgg ttatctttaa
     4261 aataattcca aatgttgttt caaattattt ttatgatttt gttgctacaa atcgttacaa
     4321 atggtatggg aaaaaagaaa gttgtatgat gccaacacct gaattaaaag ctaaattttt
     4381 ataatgtgat aaatatcatg tttaaaatca tgtaataaat ataattttga actactaaat
     4441 caatagccta tgaaaagttt aattctgcct ttatattcgc atggcaatgg gacaataata
     4501 atgattggcg ttttttcatt agttgttatt ggactggttg ttgctgtttt gctaatgatg
     4561 ggaaattcaa aaaacaaaaa agactaagca atgcttagtc tttttaagtt tatttagatg
     4621 aaaaaactat tctttaataa tttttttagt aatacttcct ttatcagtgt ttacattcat
     4681 catataaatt ccagtagcta aatcactaat atttacttga ccttctactg tatttaaatt
     4741 ttgagatttt acaactctac cattcaaatc cgttaataca acattgttaa ctactgcgtt
     4801 tattgtatta gaaatgttaa gaacattttt agctggatta ggataaacag agaaacttcc
     4861 ttctgcaata acttcagata cattcaacaa agcttcttct ggagtagctt taactgtcaa
     4921 attatcataa attatttcag aaactgcagc atttgttgtt ggtgttgtag taataaaatc
     4981 aacttcatca actataaatg gaccagccca atttgctgca ggcaatcctg tgtaaacagt
     5041 tggagttttc cagattgttt ctccagtgtt tgaatcatag gcaaaaccaa ttctgtacca
     5101 agtgtctgca tctaaaatta aacctccagt ttgtaaagta attaaaaaag tattataagt
     5161 acccgcatta ttcaaataag caactccagt caaaactcta gtgttcattg tataaacaaa
     5221 tccgtttaaa gttcttgtaa ctgatggtgt aactgaatca tctgtaccaa acaatcttac
     5281 cccaacttga gcagtactag ttgttgcagc accagtatat aaatcaaatt ctacttcgat
     5341 aatatcattt cctaaatctc taccatccca agctgttcca agaccatctt tccacatata
     5401 tcgagagcct ttatttccat tagcaccaat gatttttaag ccgttagtta cttcatttcc
     5461 attgtcaaca atcacaaaat tagaattccc agcgttatcc gatgtagttg gagctgcccc
     5521 attagttgaa aatgttaacc actccccttg accaggagta acaccagtaa tgtcagttcc
     5581 aacatcacca ttgtttaaag tattaaagtc ttctgactgt aaaacttgtg cgttagcaag
     5641 tgaaactgaa aacgcaaaca ttaagagtaa aatttttttc ataaataagt tgttttttag
     5701 ttaatttaag tagcaaattt aataaagatt ttgacataac tatttttttt aactaatctt
     5761 tttcccattt acctacaacg ctagttgcta gcgcatacct agaacgtttg taccgcttct
     5821 aaacatatca cagaaatggt caattggaag tattaaagct attccttcaa caggaatttt
     5881 aaacattcca caagtggctg ctactactac taaacttgct cttggcacac ctgcaattcc
     5941 tttactagta agcattaaga ctaatagcat tgtaaattga gtacctgtat ctaagggtat
     6001 attatatgcc tgagcaataa aaatactggc aaatgtcatg tacatcatac ttccatcaag
     6061 gttaaaggaa taacccaacg gtagcatgaa agaaactatt ttatctttta ttccaaaacg
     6121 ttctaattct tccgtcaatt tagggaatac agcttcacta ctggttgttc caaaagcaat
     6181 aaatataggt ccgatgattc tttttagtaa agttgtcata ttgcttttta ggaaaatata
     6241 accaacgact aacaacaata accataaaga tgaaataccc accatgaatg atccaaaaaa
     6301 tttggcatac gtaatcaata actcagaaac atctttaaca gcaaaaacac ctgcaatggc
     6361 accaaaaaca cctagtggtg caaaagccat tacataattt accattttta atataatatg
     6421 tgacgttttt tctaatgcaa ttacaacagg tttcgtataa tcacctatag atgctgctgc
     6481 taaaccaaag aatatggaga aaattacaat ttgcaatatt tcattggttg ccatggcttc
     6541 aacaatactt tttggtatga tgtgttcaat aaagttttgt atcgaaaaaa cttgtgtttt
     6601 atctaccaaa tctgaagctg cagtcatatc tacatctttt agatttaatc ctactcctgg
     6661 ttgtaatgta tttacccaaa acattcctat taacaaggaa atgaatgaag ccgtaaaaaa
     6721 ccaacctaat gctctaccgc caattcttcc aacggatttt atatcgccta gtttagcaat
     6781 accaaccact aaagtagtaa aaactaatgg tgcaatgatc atttgaacca aacgaatgaa
     6841 tatggttgct aaaattttta tatagccact aaacgatttt gcggattcaa cgtcataatt
     6901 attgtgaaca taaattccaa ctattgcgcc aattaacatt gcaataataa tttgtaccgt
     6961 taaattttta aaaacggatt tttttggagt ttggttagtt tccatttagt caaaaagatt
     7021 agatttttta gtgtgaagtt ttccaataga agatgctaca attcccgaaa cggcagaatc
     7081 accagttaca tttacggctg ttctacacat atcaagtggt ctatctatag caaatattaa
     7141 tgccaaacca gcttctggaa ttccggcttg tcccagaaca ataaccaaca ttaccattcc
     7201 ggcacttggc acagcagcag aacctataga ggctaacgat gccgttaaaa ctatcattaa
     7261 ttgtgctgat aaatccaaat gaatacccat agcttgagca ataaaaaccg cagcaacagc
     7321 ttgatacaaa cttgttccat ccatatttac tgttgcacca agtggcaaga caaaactggc
     7381 gacttcatca tcaacgccta agttttctgt aacacattcc atcgtaacag gtaatgttgc
     7441 tgcacttgaa ctggtagaaa aagctaacaa ttgtgctggt gcaatggcat taaaaaaacg
     7501 tttgggtttt acttttgcaa aagaatataa taatgttgga taaacgataa acatcattaa
     7561 acctaatccc aaaaccactg ttaaggcgta aactaataag gcttgaagcg ttgtaaaatc
     7621 aggaatttca acaattaatg cagccattaa tgcaaacaca ccaattggtg ccgcaagcat
     7681 aataaagtca atcattttaa gaataacctc gtttaatcct ttaaagaatt ttacaactgg
     7741 ttttgcttgt tttcttttaa tactaattaa accaattccg attaagatgg caaataatat
     7801 cacttgcaac atgctagcgt tatccgtcat tgcatgaaaa acattgcttg gtacgatatc
     7861 aattaatggt tgtaatggtc cattattttt ggtttgggat gcaatttcaa ttttttgagc
     7921 cgcatcggaa gcaaaatttt gtaataaact ttctcgagat gcttcattaa taaaacttcc
     7981 aggattgatt acattagcca atattaatcc gattgtgact gcaattacgg ttgttgataa
     8041 atacagactt attgttcttc cacctagttt tgataatttt gaaatgtctt tcaaatcggc
     8101 caaaccattt attaatgaaa ctagaattaa tggaaccgca atcattttga gcaaattgat
     8161 aaagatagtt ccaaatggtt ttatccaatc aattattaat cctttttgct ctatgctttt
     8221 catcaataag ccaaatagca aacctaaaat cataccgata agtattttcc agtgcatttt
     8281 aagtttgaaa cctctcattt atgaatatat tttattaatt agataaaaat cggttaaaac
     8341 caaagctgcc atagcttcta caattggaac agctcttggt actacgcacg catcgtgtct
     8401 tccttttcct tgttgttctt caattgtagc tttattggtc agtacttctt gtttttgcaa
     8461 taatgtggca actggtttaa acgcaactct aaagtaaatg tccattccat tagaaatacc
     8521 accttggatg cctccagaaa gattggttct tgtagttcca tcttcgttat acaaatcatt
     8581 gtgttcgcta cctttcattt tggcaccaca aaatccgcta ccgtattcaa aaccgtgaac
     8641 ggcattaatg gataacattg cttttcctaa ttcggcatgc aatttgtcaa aaacaggttc
     8701 gcccaatcca acaggtacat tttgaatgac acacgtaatg gttccaccaa cagtatcgcc
     8761 ttgtttttta atctctttga tatactcttc cattttctgc gctgtgtcgt aatcaggaca
     8821 acgaacagca ttggtttctg ttactgaaaa atccaatgat tgatagggtt tgtctataaa
     8881 tatttctcca actgaggaaa cgaaagcatt tattttgatg tttgaaatca cttgttttgc
     8941 tatggctcca gctgctactc tacttgcagt ttcacgagcc gaacttcttc caccaccacg
     9001 ataatcacga ataccatatt ttttttcata tacataatcg gcatggcttg gtcgatagct
     9061 gtcttttata tgtgaataat catctgactt ttgattggta ttttgaatga taaatccaat
     9121 gggtgttcct gttgtttttc cttcaaaaat tccagagaga aactgtacct catcttcttc
     9181 ttttcgttgg cttactaatg ttgattgacc tggttttcgg cggtgcattt ctttttgaat
     9241 ggcttcaaaa tctaatacaa tccctggtgg acaaccatca attattccgc caagtgcttc
     9301 gccatgcgat tcgccaaatg tggtaagttt aaatagtgtt ccgtaactgt ttccagccat
     9361 tgttttaagg ttttgaacaa atatagtttt ttgtacttag tttttaaaac tttgtaacta
     9421 ttttaaataa aatggatttt acaattcggt aatggtttta tgaaaatgat ttaacaataa
     9481 tgcgacatcc aattaatatt caaatcttta atttgtaaaa cttcaataag aatgaaaaaa
     9541 agaaaacttg atttagttgt aatttcagac acacatctcg ggacttatgg tgcacatgct
     9601 aaagagttgt accattattt aaactctatt aaaccaaaaa cactggtgct gaatggtgac
     9661 atcattgata tttggcaatt tagaaaatcc tattttccca aatcacattt aaaagtgatt
     9721 aaaaaaatta ttgatttggc gtcaaaagga accaaagtct attatttaac cggaaatcac
     9781 gatgaaatgc tccgcaaatt tagtcctgct acactgggaa attttatttt agccgataaa
     9841 gttgtgttgg atttagacta taaaaaagca tgggtttttc atggtgacgt ttttgatgct
     9901 tccgtgcaac actctaaatg gatagcaaag ctcggcggtt tgggctacga ttatcttatt
     9961 gtattaaata gaatggtcaa ttggtgtttg aaaaaaatga gaaaagaacc ttattcattt
    10021 tccaaaaaaa taaaagcaag tgtcaaaaaa gcagtaaaac acatctctga ttttgaaaac
    10081 acagccacgg aattggctat cgaaaataag tatgactatg ttatttgtgg acatatacac
    10141 gaaccaaaaa taacaagaca agttcgtaaa gatggaaaca cgatatactt gaactctggc
    10201 gattgggtag aaaatttaac tgcactagaa tacaaaaata aacgatggaa actttaccgc
    10261 tatgaagatc ataccgaaaa agaggatgaa gacttatttg aattggatga tttaatcaaa
    10321 catcaattaa ttgcttctat ggttatgaag caacctttga tgtaataatc acctatctta
    10381 ttttttcctt aattatataa ttatgttaaa aaaaacagaa attatataac aaaaagtaac
    10441 tttcttcgtt atagatttgt aaaagtaaaa acaaacaaat aacattatga aaaaaatcat
    10501 tttatctgca tttatgctaa ttggattagc atttgcaaca caagcacaag atatttctaa
    10561 aaatgcatta ggtcttcgat tgggtgacaa cgatggattt ggtggtgaag tatcctatca
    10621 aagaggctta ggcgataaca acagattaga acttgattta ggttggcgca atagcaaaaa
    10681 ctatgatgca attaaattag taggtttgta tcaatgggtt tggaacatcg atcaaggttt
    10741 taattggtat gcaggtgttg gggctggtgt tggaagttgg agctacaata aaagtggcca
    10801 aagtgcgagt ggaacattta ttttagccgc tggagatatt ggtattgaat acaactttga
    10861 tataccgctg caattgtctt tagatttaag acctgaattt tatttaaaca ataatggaaa
    10921 taatcaattt agagaaaaca attttggacc agattttggt ttaggtattc gttatagatt
    10981 ctaattcaaa atattataat cacaaaaatc ccaattgtat tgggattttt tatttaaaaa
    11041 ttattggttt tttagaattt atcttaacaa cactataggg atatgtagtg ttttcatcgg
    11101 ctacattagc ttcttttatt tttaattctt taattttcaa aataatattg gttggtgttt
    11161 cctctacttt ttctaatgta gttgtataat tcccattggg taatggtcct aagtttatga
    11221 tgatatagtt acaagaaagt atatcgtctt ttttaatcaa ctttctcaaa tcgttatcgc
    11281 ccatgagcat gtttatctct tttggttccg aaatgatttc ataaaactta atattagcac
    11341 caccatcatt cttggttact aaaacttcat acaatggttt tttaaccatc tcttttttag
    11401 aagtcgaaca agaaaacaag gttaatccaa taagcaatag gcaaattctt ttcatcatga
    11461 ttgcacaatt gatttagagt tttgaatggc taatctatac aaatcttcat aaagtggcaa
    11521 aatgtttttg atgtcaaatt gttttgctac acttaatgca tttgctttaa attcatttaa
    11581 aatagtttcg ttttccaaaa ttttcaaggc attttctgcc atatcatcta cgtttccaac
    11641 atcgctcaaa taacccgaaa caccttcaaa attaacctct ggcaatccgc ccgaattact
    11701 agacactact ggtacactcc atgccattgc ttccaaagct gccaaaccaa aactttcggt
    11761 ttccgatggc aataagaaca aatcagaata ggataatatt ttatctattt cgttactgtt
    11821 tccaaagaaa atcactttgt cttgaatgcc taatttttga cacaatgctt ctgctttttc
    11881 cttctctggt ccatcgccca ccatcattaa tttggcaggt attttttgtt gaattttatt
    11941 gaaaatctga atcacatcgg gaattcgttt tacttttcta aaattactaa tatgtgtaat
    12001 tattttttcg tctttggatg ccattactga gcgttgacac gaaatttgtt gctcgtttct
    12061 atgcttgtct aattcgataa aattaggtat tacatgaatt tctttttgag tatcaaacaa
    12121 agcataggta tcttctttta aactttgaga aaccgaagtt acaatatcgg atttattgat
    12181 actaaaattt acggctggtt tgtaaaacgg atgattgcct accagagtaa tgtctgtacc
    12241 gtgaagtgtt gttatcatag gaatatttat accttcatct ttcaacatct gttttgccat
    12301 atacccagca taggcatgcg gaatagcata atgcacatgc atcaattcaa ttttatggag
    12361 ttttaccata tccaccaatt tgcttgaaag tgccaactca taaggttgat agtgaaacaa
    12421 aggatattcg ggtacattta cttcatgata atgtacattt gggtttaaca atgccaaacg
    12481 tactggttgt cgataagtga taaaatgtat ttcatgaccg cggtgtgcca attctaaacc
    12541 caattctgta gcgacaacac cactaccacc aaaggtagga taacagacaa ttgcaatttt
    12601 catgtagatt ttttagaaat acgaaattac acaaaatata acggagaagt gttaaaatat
    12661 cgttgtaatg ttatttttta ttgatacaac aaaccacatc tttatggatt gcagtagata
    12721 aagatacttt gagtgatagt gtttatttga gtgatagtgt ttattataca aaaaggctta
    12781 acttattgtt atatttattc aattttttgt tgggttcttt taaaaaaata tgaaaaataa
    12841 tgattttatt taatctcgac ttcaaactta taatttataa cacataactt ctcactcata
    12901 tttattaatt atatttgtgt caactaaatc gatatagtaa tggaagaatg tatttcagta
    12961 tttgatatgc ttaaaatagg cgttggacca tcaagttcac acacacttgg accttggcgc
    13021 gccgccgaac gttttttaag agagctgcat gaagaaaaaa tacttgacac tattctaact
    13081 ataaaggttg atttatatgg ttcgctatcc ctaaccggaa aaggacatgc tacagattta
    13141 gctgtaatgt tagggctaag cggtcaagat ccagtaacaa ttcccgttca gaatattgac
    13201 acgattatta aaaaaattca aaaaacaaaa caattacttt taggaaatca acatactatt
    13261 gcttttaatc ctgataccga tattgttttc aatagagaat ttctaccctt ccatgccaat
    13321 ggtatgactt tcaccgccac caccaaattg ggtaattaca ccaatactta ctactctatc
    13381 ggtggaggtt ttgttgtagt agaagaacga ctaaacgcca agaaaaagat ggaatttaag
    13441 tgcgcatttc cttatccgat agacaatgcc aacatgcttt tgaagtattg taccgatgaa
    13501 aacaaatcga tttccgaaat tgtaatgatt aacgaaaaat ccatgcgttc cgaaaaagaa
    13561 atcaacgaac aattgatgac catttggcac accatgttgg aatgtatgta catcggttgt
    13621 cattcggaag gcatcctgcc aggaggtttg aacgtaagac gcagagcttt tgatatgcat
    13681 caaggtttaa taggacttgc caattattcc aatcctcaag aatggttaga atcgattaga
    13741 aaaacagaag taaagtttcg ccaaatctta aaatgggtat cgtgttttgc tttggctgta
    13801 aatgaagtca atgcagcact aggaagagta gttactgctc caaccaacgg aagtgcaggc
    13861 gttattccag ccgtattaat gtattatctt gtaatcgaaa accacaacgc tggcgaaaaa
    13921 gaaatcaaac aattcttgat ggttgctggc gaaataggta gtttgtttaa acaaggttca
    13981 accatttctg ccgctatggg tggatgtcaa gccgagatag gtgtttcgtc tgctatggct
    14041 gccggcgcac tgtgcgaagt gatgggtgga actcccgacc aggttttaat ggctgccgaa
    14101 attgctatgg aacatcatct agggttaacc tgtgacccaa ttggtggatt ggttcaaatt
    14161 ccgtgtattg agcgcaatac tatgggtgct atcaaggcca tcaatgctgc cgaacttgcc
    14221 tttgaaaccg atgctaagaa tgccaaagtg cctttggaca aggtgattaa cacgatgtgg
    14281 caaacagcaa aagatatgaa ctcaaaatac aaagaaacct cagaaggtgg attggctatt
    14341 gcggtgaata tggcggattg ttaataaatt tatcgctaaa atttagacca tttatacaat
    14401 tctttccacg aaggtttttt gccatacatt aaaataccta ctctgtatat ttttgaagca
    14461 aaccatacaa ccaatataaa cgttccgaat aataacacca acgaaattac aatctgccat
    14521 aaaggcacac caaacggaat acgcatcatc ataacgattg gagaggttaa tggtatcatg
    14581 gagaatactg tagcaatagt gccatgcgga tcattaatta cggtgaaaaa gccaatataa
    14641 acgcctaata ttaaaggtag gataattggt aataaaaact gttgtgagtc ggtttcgtta
    14701 tccacagctg caccaattgc cgcatagaat gaactataca aaaaataacc cccaataaaa
    14761 taaagcatga atgagactag aagggttgct attggtaaat tccatatctc ttgaaaataa
    14821 agttgtacat tactcatgtt ttttgatgcc atttccatag cttgttgtga ctgagcacca
    14881 gtagcaccta cttgaacgcc aaaaacagac gataaaataa acatagccga tagaccaagc
    14941 aatgcccaaa ttaaaaactg aagaattcca gctaaggatg ttccaataat tttgcccatc
    15001 attagttgaa atggtttaac cgaagaaata attacttcga taatacgtga agttttttct
    15061 tcaataacac tgcgcataac catgttacca tataatacaa taaacatcat aattaaatag
    15121 ccaaacgcac caccagcaat ggtttttatc cagtttaacc cttgaatgga tttttcacca
    15181 gaagctttct tgatgttaat agcaaccatt gattttaatt ctttaatctt taaggtatct
    15241 aatccgtttg atgcatactt ttgttgcgtg atggactgac ctatggtttt ctctaaatca
    15301 gaaataatat caaaatcggg actgtcatta gaaatataat caatgctttt agctaaagaa
    15361 tccggattgg ctttctttgg gattaaaagt aatccttcgt aggcttcaga aataatgctg
    15421 tcttttaatg ttttaacatc aattaatgag aaatccgtgt aatagtattc gtcgctgttt
    15481 tttaatagtt taaaataatt tcctgtttca tcatgaacgg cgatggtttt aacttcgggt
    15541 ttcatcgtac ttaaaaagcc aacgaaaact ccaattccaa caaaaagtaa cggactcaaa
    15601 aatgtcatta caatgaatga tttgttgcga acttttgcaa gaaattctct tcttatgatt
    15661 agggttaatt tgctcatagt attttattaa aagcacttcg actgcgctca gtgtgacaaa
    15721 attggaattt ggtattatat ttttttggta ttttactcag ttactgtttt aataaaaata
    15781 tcatttacac ttggaatttt ttctacaaaa tgagtcacct gaccgcgttg caccaaagtg
    15841 tttagcaact cattaggtgt agcattacct aaattgatgg ctaactttaa ttcgtcgttt
    15901 aacgatttga aatccgtttg cgacaaagta aatttctgag ttaaatcaaa catcaaacct
    15961 tctacattgt ccgtaagtat gccaacttca taagtgttgg ttcggtattc tttcttaaca
    16021 tcggataatt ttccttctat tagtttattc gatttatgaa taagtgcaat atggtcacac
    16081 atttcttcaa cgctttccat tcggtgtgtt gaaaagataa cggatgttcc ttgtttattt
    16141 agttcgatga tttcatcttt aatcaaattg gcattcacag gatcaaaacc cgaaaatggc
    16201 tcgtcaagaa tcaatagttt tggcttatgc aaaacagtaa ccacaaattg aattttttgt
    16261 gccatacctt tagaaagctc ttgaatcttc ttgttccacc agccttgaat ttccaatcgt
    16321 tcgaaccaat actttaattc tttggtagct tccagtttgg ataaaccttt taattgtgcc
    16381 aaatacaaac actgctctcc gactttcatg gttttataca aaccgcgttc ttcgggcatg
    16441 tatccaatat tttgaatatg atttaaattt aatttctctc catctaaaag aatttcaccg
    16501 ctatcgggca atgtaatttg attgatgatt cgaattaggg atgtttttcc agctccattt
    16561 ggacccaata atccataaat acttcctttt ggaatggtta atgaaacttc gttaagtgcc
    16621 gtaaaatcgc cataggtttt tactacattc ttaacttcaa gtatattgtt cataaataaa
    16681 taattaaatt ccaaataatt aaaactccaa attctaattt gaaattgatt ttgctattga
    16741 aattgacttt tgaaattact tttgtaaaag tactatttta gtattgtttt tttttaattt
    16801 tttcacaaaa aaacccaccc aataaaatga attacaggat gggaaaaatt gctatgaaaa
    16861 agaaatacta gtttcctagt attatccaaa tgtataatta atttttcaat tatgaaaaca
    16921 tatcttttac tttttcaaaa aaagatttat cacttttttc tggatgtggt agaaagttat
    16981 catcagtcaa agatttttca aagaattgac gttgttcttt attgagtgtt tttggtgtcc
    17041 aaacgttgat atgaactagc aaatcaccgt ttccataact gtttaaactt ggaattcctt
    17101 ttccttttaa tcgcagtatt ttacccgact gaataccttc gtccaactta attcttactt
    17161 ttccatttac agcttcaatt tctttggaaa ccccaagaac tgcttctgca atacttatat
    17221 ataaatcata atgaagattt tcgccttcac gttttaaaaa ttcgtgttct aattcttcaa
    17281 tggcaacaat caaatctccg gcaattccat ttcctggggc atcatttcct tttcctgaga
    17341 cttttaattg cattccgtca acaacaccag ctggaatttt gatagaaacc gtttcgtctt
    17401 ctaaaatcat tccatgagca tcggcattat taggtttgct ctcaatagtt tgaccagaac
    17461 cgccacaaac atgacacgtg gtagctgatt gcattcttcc cagaatggta ttggttactt
    17521 tcaaaacttg tccttgccca ttacatgtgc cacatgtttt atatttaaca cccggtgctt
    17581 gaatttttcg tttaacctta actttctttt caacgccatt ggcaatttct tctagagtta
    17641 attttacttt aatccgaaga ttacttcctt tcactctgcg ttgtcctcct gaatttccac
    17701 caaaaccacc accaaaactt ccaccaaatg cacctccaaa aatatctcca aactgactga
    17761 aaatatcatc catattcata tgatgtcctc cgccaaaacc tccagcacca tcaaatgcag
    17821 catgtccata ttgatcatat ttggcttttt tatcagcatc acttaacact tcataagctt
    17881 ctgcagccaa tttaaatttt tcttcagctt ctttgtttcc tgggtttttg tctggatgaa
    17941 actcgattgc ttttttgcgg taagcttttt ttatttcttc agtcgaagcg cttttggtaa
    18001 cgcctaaaat ttcgtaaaaa tcttttttca tttttctatt ctaaattaaa ttccctttga
    18061 aatgcttggg tgacagcgag aattttaagc tcctaacact acttttggaa aacgaataat
    18121 tttgtcgcca agtttatacc ctttttcaac tacatctaca atttttcctt tcaattcttt
    18181 gctcggtgct gcgatttggg taattgcttc ggcaaaatca gcattaaaaa catcacctgc
    18241 ttttagttct actatctcta atccttttga ggctagagtg gattttaatt tttcatgaat
    18301 taattctaca ccttttaata acgtttcatc ttcgctttta gaaatttgaa tcatagctct
    18361 atcaaaatca tctaaaactg gcaacatggc ttgcaaaact tcctgattgg cagttttgta
    18421 taaatcaaat cgttctttgg tagttctttt tttatagttt tcaaattcag cgaaaagtct
    18481 aagaaattta tctttttcat ttgccaattc ttcttgtaat tgttgctcaa cagttaattc
    18541 ttctgctgct tcaactccat tgaaactatt ttcttgttcg ttttctatag gttctttttc
    18601 tgtattatca gtattcattg ctttttttgt tttaaaaaca tctttaaatt tcatatcgtt
    18661 ttactggatt gcaaaagtac tgccatttct taaaaaatgt caaattgtca ttactttttt
    18721 ttatttttaa aatatttgaa acttttattc ccttttttag aaaaattaat gcaattttaa
    18781 ggttatatcg atttcgaata tactatattt gtgatagtac aattaaatga atgtactgcc
    18841 aaaggttata aaacttatta attcaaatat caaaaaggta cttaattaaa ctcaagtacc
    18901 ttttttttat agtaattctt tcaatgctgc attaaaattt ggataaataa atgaaaaccc
    18961 ttttttacga attttttcgg aactcaaatt tttactggta aaaagtaaaa ttgacatttg
    19021 tcctaaaact attttcatca aaaactgagg aatatttgga agaaataacg gtttattcaa
    19081 atactttgca atgctttttg tctgtgtttc attagaaata gtttctggag caatggcatt
    19141 gtatattcct tctaattgtt gttcagcaat gaaacaatag agattaacta aatcgtcgat
    19201 gtgaatccaa gattgcattt gttttccatt tcccataatg gctcctacac aaaattttat
    19261 tggttttaca atttcttgaa atgcaccgcc acttttagca tatacaacgc ctgttctgat
    19321 ttttgaaact ttaattccca gtatttcaaa ttggttggct gccttttccc aaactttaac
    19381 tacttctgac aaaaaatcgt ttgcagttgc tgtagtattt tcatcgtgta ctttttcaaa
    19441 actttcagga taaatagctg ttcctgatgc tgaaataaaa tgactaacat tatgatttgt
    19501 atttttcaat gtagaaaaaa gtaaatcagc agttttggtt ctacttgaaa aaatttcttt
    19561 tttatacttt tttgtccagc gttttgcaat acttgcacct gccaaatgaa tgattgcttc
    19621 tactccttta agacaatcag tgtcaatttc attttgttca ggattccaat aaaatcctct
    19681 ataattaggc tcacttttga gtttgttttt tgaagtggtt aagtaattaa tggtgtgccc
    19741 gtttttgtga agttgtttca ctaatgcggt tccaattaat cctgttgcac ctgtaattag
    19801 tagtttcatt tataaagatt taatcaaaaa tacgtttaaa agtggttagt tatcaaacct
    19861 ttttaatgag tttaactttt gtttaaactt taatctttac gctccattca aaatccattt
    19921 ccgaaacttg ttcgcctttt tgattgacgc caattgactt catccaaatg gtttgtcctt
    19981 ctctagtggc aattgttttt ttgatagctt cttctattaa atgaccgtca ttgcaagtga
    20041 aagttatcct gccagtagct ttcttagaat agtttccttt attattagca acaagcattg
    20101 aaattttttg gttactttct ctgattttca tcataactaa cgctcctgtt gtcaattctg
    20161 cagccattgc ttgaacagca aaatacattg aattaaacgg attttgatta aaccacctgt
    20221 gttttacagt aaccacacaa ttgtcttttg aaatggactt taatcgcact ccagaccaaa
    20281 atgcagaagg aagcttaaag aataaaaatg tgtttagttt aataggtgta attggcatga
    20341 agtactggtt ttgttgtaaa tatagtcttt tttaaataaa atattcaaaa aacagaaatg
    20401 ttaattatat gttaaattta agtactttgc attgcacaat accttttttt aaccttatct
    20461 ttgtataaga aattaatagc caatctatag tatatcctaa aaacaaattt caattatcaa
    20521 taaaatcaat caaaaaatga catcaactaa cgacagaaac acggcaacat tcattcactt
    20581 gagtacattg tcacagtata ttattccgtt tggaaattat atcattccta ttatcatttg
    20641 gagttcaaaa aaaaatgaat cgcaatttat agatgtaaac gggaaaaatg ccattaattt
    20701 tcaactaagt atgtttttat atacgatgat taccttagta gttgctattc cagtttttat
    20761 ttatagtatt ttcaacaata ctacaataaa aaatattcat gatggccgcg attttattgt
    20821 tgatcacctg aatgctggaa atatttcggg aatcattatt ttagcaataa ttactgtttt
    20881 attattttgc tttttaaaag taattgaatt tatactcatt atatatgctt cggcaaaagc
    20941 ttcaaatggc gaagcatata aatatccact aacaatcaat tttataaaat aattaatcat
    21001 tcaatcatca atcaaaaaat gaacacgtta atcaattctg aaaaatattc agattaaatc
    21061 aaaatcgaat tatgaacatt gaaaacacaa aagctcagat gcgcaaagga gttttagagt
    21121 tctgtatctt atctatcatt aaagaaaaag aagcctatac atcagatatt ctagaaaccc
    21181 taaaagacgc taaattgcta gtagtagaag gaacagtata tccgctacta acaaggttaa
    21241 aaaacgacgg attacttagc tatcgctggg aagaatcaac atctggtccg ccaagaaaat
    21301 attatggtct gaccgaatta ggaaaaacat ttttaaacga attaaacggc acttggactg
    21361 aattgtctga cgctgtaaac atcatcacaa ccaaaaacaa ctaatcatga acaaaacagt
    21421 aaatattaat atcggtggtt taatttttca tattgatgaa gatgcatacc aaaaattgtc
    21481 aagatatttt gaggcaataa aacgatcact ttcaaattct tctggtcaag atgaaataat
    21541 gaaagatatt gaaatgcgtg tggctgaact attgacagca agacaacaat cagacaaaca
    21601 tgttatcaat aacaaagatg ttgaagaagt tatagctgtt atgggacaac ctgaagatta
    21661 tcgcattgat gatgatacag aacaaccaaa aactgaaacc tattatactt cttcaagtcg
    21721 aaaaaaatta tatagagata aagatagagg aataattgca ggagtttgta ctggtttagg
    21781 acattattct ggagttgatg ctgtttggtt taaaatatta tttttaatct tcgtatttgc
    21841 aggatttgga actggaataa ttgcctactt tattttatgg attgctgttc caaaagcaat
    21901 tacaacatca gagaaactag aaatgactgg gcaaccagtc actatttcta gcatcgaaaa
    21961 aaaggtaaga gaagaatttg attctgtttc cgaaaaaatc aaaaatgctg attatgacaa
    22021 attaggaagc caagtaaaat atggtgctga aagagttggt aatggtgttg gagatgtgtt
    22081 aattaaaata tttggtgcct ttgcaaaagt acttggtgct ttaattcttg tgttttcttc
    22141 tatgtcattg gttggacttt taattggaat gttcactgtt ggaagcacct cgttcattaa
    22201 cactccgtgg cagcgatata ttgatgcggt aaattatact gatgttccgc tttgggcatt
    22261 tagtttatta tcatttcttg ctataggaat accattgttt ttcttcttat tgttaggttt
    22321 aaaactaatg gtaaacaata tgaaatctat tggaagtcca ataaaatata gcttcttagc
    22381 catttggata tttgcagttg gtgccttggt cttcatcgga atcaagcaat caacagagag
    22441 agcttttgat ggaaaaatgg taaccaaaga aatgataaac atacaatcta acgacacttt
    22501 gagaataaaa tttgtgaata atgatttctt ttcaaaagat attaataatc atgaagactt
    22561 tctatttaca caagactctt taaaaaacga agtgatttat tctaatgaag ttaagtttat
    22621 tatcaaaaaa acagataaac ctcaacctta tcttcaaatt gaaagattag cagaaggaaa
    22681 atcatttaat gaagccaatc taagagccga aaaaataaaa tattctttca aaattaatgg
    22741 aaatcaatta cttttagata attatttact aactgaaatg gctagtaaat atagaaatca
    22801 aaaagtagaa ttgtatttat acttgcctga aggaattgta tattatcctg acgaaaatgt
    22861 aaaatatttt ttagaaggat ctaattctga ttttgattat tattatggcc cagaaggata
    22921 caaatacaaa gtaaaaaaca ccgatttaat ttgtttaaat tgtccagatg atgaaaataa
    22981 tgatgtaaaa acagaagata tcaacgcttc tgatgaaaat gattctataa aaacagttac
    23041 tgtaaaaatt aatggcaaag ttgtttctga aacaaaatca gacaaaaaag gaagtttaaa
    23101 aataaacaaa gacggagtaa taattaaaac caattaatca tgataaaatt tgtaatgttt
    23161 tgtgtaaaag cctttgttgc aactctggtg ggtttgttag taacttcatg caacggaaaa
    23221 attgatttag gaaactccat caccggaagc ggaaaagtgg tgaaagaagt tcgaaattta
    23281 gattcttttg acaaagtaac agtgtgttgc ggattggaat gtgaaatcat acaagcaccc
    23341 aattttaaag taactgttga agctgacgac aatcttatta aagacataaa aacgtctgtc
    23401 attaatggca ctttaaaaat ttcaacagat tatggcaatt atgtaaatgt aacttctaaa
    23461 aaaataattg ttgaaatgcc gaatgtaaaa agcttagaaa caactagtgg ttcatcactt
    23521 aaaactataa aaaccattcg tgaaaactca ttaagtttaa aaacaagtag tggaagtgaa
    23581 atgaatgttg aaaccgaatc tgacactatt agactggaat caaccagcgg aagtgaacaa
    23641 aaaattaaag gaaaagcatt ggttttattt acttcatcat caagcggaag cagtattgaa
    23701 gccgaaggtt tgttagcaaa tgaaatttca tcgcaatcca ctagcgggag ttctactgaa
    23761 gtaaatccaa tagttagttt aaaagcaaaa gcatctagcg gaagttctat tgactataaa
    23821 aacaaaccga agtctatatc caaagaagaa agttctggag gaagcgtttc aagaaattaa
    23881 tcagtaataa aagcatctca attcgcattg ggatgttttt ttttctattt ttggaaaaac
    23941 atttagtttt ataacaatga aaaaaagttt atccttacta cttcttttgc ttgtaacttt
    24001 ctcatttgct caaagaaaag aaaaagtaaa aggctccaaa atagtaaaaa tggaacaaaa
    24061 acaagtagaa agtttcactt ctttagaagt ggaagacaat cttgaggtat ttttggtaaa
    24121 aggaaacgaa tgtggtcttg aaattgaagc cgacgataat cttattgaat ttattgacta
    24181 taaaagtgct ggaaacaacc taagaatttc aacaactcgc gacatgaata gttttaaaaa
    24241 actaagtgtt agggttactt atacttccga tttaaatcta atcatcgcta aacacgaaac
    24301 caatgtaacg gcattatctg atgttgattt ggcaaatgtt acattcaaaa gttacgatta
    24361 tgcaaagctt tttttaaatg cgaaaaccaa agattttaca ttgatggcaa acgacaaatc
    24421 taagattgaa cttaatttaa agtcagaaaa aacaactatt gatattagta aaaatgctga
    24481 actaaaagca ctaatttcat caaaagattt aggtattgat atgtatcaaa aagctgaagc
    24541 aaaaattgaa ggtgatgtta ttaattttaa actaagagcg gataacaact ccaattttga
    24601 cggcgcaaaa ttaactacaa caaatactca aatttttgca gatgcctatt cgagtgctaa
    24661 aatcaacgta aaaactaatg taattattga tgccgcagga aaatcagaaa tacaactttt
    24721 tggtgaacca aaaatagaga tcaaaagatt tactgacagt gcaaccttag aaaagaaacc
    24781 cattaaataa aaaaaatcct gaactgcttc aggatttttt ttataaattg taatgattaa
    24841 tccatagatg ctaaataacg ttctgcatct aaagcagcca tacaaccagt tccagcagcg
    24901 gtaaccgctt gtctataaac atgatcggca gcatcgccac caacaaaaac accagcaaca
    24961 tttgttttag aagtgccagg aatatttttg atataacccg tttggtctaa ttctaaaaaa
    25021 tctttaaaaa tatcggtgtt tggcttatgt cctatagcca cgaaaaatcc agtagctggt
    25081 atatcaattt cctcgttagt tgttttattc aatgctttta ctgaggtaac aacttgtcca
    25141 tcaccaataa cctcaaccgt atcatgattc attaaaatag tgatgttttc cgttttctta
    25201 acgcgttctt ccataatttt agaagctcta aacttatcac ttctaaccaa catggtcact
    25261 tttttacaca attttgataa gtaatgtgct tcttcacaag ccgaatcacc agctccaaca
    25321 attactactt cttgatttct ataaaagaaa ccatcacaaa tagcacacgc agaaacgcca
    25381 ccaccagttt gcaaataatg ttgttcggaa ggcaaaccta aatattttgc cgatgctcca
    25441 gtagaaataa ttactgtttc tgcatgaatt tctttagagt cattaatcca aactttatgg
    25501 gtttttccag aaaaatcaac ttttgttgcc caaccatcac gaacatccgt tccaaaacgt
    25561 tgcgcttgtg cttgtaattg aatcatcatt tctggaccag taacaccatc aacatatcct
    25621 gggaagtttt caacctcatt agttgtagtt aattgtccac caggttgcgt tccttgatat
    25681 aaaactgggt tcatattagc tctagctgca taaattgcag ccgtatatcc cgcaggacct
    25741 gaacctataa ttaagcactt tactttttct attgtgtctg acattgtata aattatttta
    25801 aaccgcaaat gtaagttttt ataaaaatat atcattgtaa actaccatac aaaaatctat
    25861 tttattataa acaaatatta ttatagtaaa aatttttgag aactcattaa tagttttata
    25921 tttgcactca cttttcgggg tgtagcgtag cccggttatc gcgcctgctt tgggagcagg
    25981 aggtcgcagg ttcgaatcct gccaccccga ctaaatcctt ttcatatttt tttggaaagg
    26041 atttttttta gtctttttat tttagaaaac caaaaagctc tcgatataat ttctattttt
    26101 gttccaacac aaaaaacaca actttttatg cttattattg gaattgctgg cggaactgga
    26161 agtggaaaaa caaccgtagt tcaccaaatc atgaatgagt tacctcaaac agaagtaggt
    26221 atcatttctc aagattcata ttataaagaa aacaatactt taagttttga agaacgtgcc
    26281 ttgattaatt ttgaccatcc tagagctatt gattttgaac ttttagttca gcatttaaaa
    26341 gaacttaaag aaggaaatac tattgaacaa cctatatatt cattcataac tcataataga
    26401 acggatgatt cagtcattac tcatccgaga aaagtaatga ttgtagaggg aattttaatt
    26461 ttatcaaatc cagaacttag agatttattt gatgtaaaaa tatttgttca tgccgattct
    26521 gatgaacgtt taattcgccg tttaaaaaga gatattgcag agcgtggtcg tgatatggaa
    26581 gaagtattga accgttatca aactacgtta aaacctatgc accaacaatt tatagaaccc
    26641 actaaagctt ttgccgacat cattattcct aatgacaaat acaatactgt agctatcgat
    26701 gtggtccgtg ccgttattaa ccaaagaatt acgtaacttt acgataatta ttttttaatg
    26761 agttatttta caaacatcat agacaaatat cctattctca aaatattgag caataggttt
    26821 gttattgttt tgattttttt tgcaatttgg atgattttct tagacaatac ttcttatttt
    26881 gatcatagag tattgaacaa agaactcgaa gaacttgaag ttaacaaaaa atactatcaa
    26941 gatgaaatca aaagagatga agaagacatc aaacgcttaa aaaatccaga tcaaattgaa
    27001 aaatacgctc gtgaaaagta ttatatgaaa cgtgacagtg aagacattta tatcatagaa
    27061 tatgaaggcg acacgattca cgaagaacaa tccaaaacga aatcattata agtctatctt
    27121 atggaaaata aattatttga ttcctttgaa acaataagta ccaaacagtg gaaacaaaaa
    27181 atccaatacg aacttaaagg tgcagattat aacgacacct tagtatggga aagtttagaa
    27241 ggaattaaag taaaaccatt ttatcacagt gatgaaatgg aaaataaatt ttcttttaat
    27301 gaggcaaaac ctttttctat actccaaagc atctatgttc atgatgttgc taaatcgaac
    27361 aaacgcactt tagaaaccct aaaccgaggt gcggaaagta tccgatttac tatcgaaaat
    27421 gaaactattt cagttaatga attgatggaa aatctcccta aaggaaatac aacctatttt
    27481 ttttatttgc cttttctatc aatctctttc attaacaaat taaacgattt tagtctagat
    27541 aacggatatg attttaaact tcaaatcgat ccaatttatc aactatgtaa agatggaaat
    27601 tggtttgaca acctttataa ggactttgat atactgaatc aaattgcatg taaagccaaa
    27661 tcgagcttca ttactattga cagttcaata tatcaaaatg cgggtgccaa tatcgttcag
    27721 caactagcct acactttagc tcatgtcaat gaatatttca accgaatttc gaagatcaat
    27781 caacctataa ctattgaaat ttccgtggga agcaattact tttttgaaat tgctaaactc
    27841 agagcattgc gtttactttt caatacactt accaaagaat acaatcacaa tttggaatgt
    27901 atcatcattg caactcctac caaaagaaac aaaacccttt acgattataa tgtaaacctg
    27961 cttcgtacta ctaccgaatg tatgagtgca atattgggaa atgctaattt tatctcaaat
    28021 cttccttatg atactttgta tcataaagac aacgagtttg gcgataggat agcacgtaat
    28081 caattattga ttctaaagca cgaaagctac tttgacgaag tgactaatgc agccgatgga
    28141 agttattata tcgaagaact tacagcgcaa ctagccgaaa aagcattgtt attatttaaa
    28201 gaaatagaag ccaatgatgg cttaattacc caacttattg caggaactat tcaaaagaaa
    28261 ataagcgaaa gcgctgcaaa agaacaagaa tggttcgaca gcggaaaaga agtgttatta
    28321 ggaactaaca aatacccaaa caaaaacgat aaaatgaaaa acgatttgga attataccct
    28381 ttcgtaaaac aaaaccctcg taaaacatta attgttccaa ttatcgaaaa acgcttagcc
    28441 gagaaactag aacaagaacg attagctcag gaacaataaa ctcatgagaa aaaacatcca
    28501 acatattaca ttagaaaatt ccaaaaaaga atttccaaat ttcaacaatt cggaactcag
    28561 aactttccaa accgcagaag gtattgaact aaaaccaacc tattctgaag atgacatcaa
    28621 agaattagaa cacctaggtt ttggagctgg ttttgcaccc aatctccgcg gaccatatac
    28681 gactatgttt gtgcgtcgtc cttggactat tcgtcaatat gctggtttct ccactgccga
    28741 agaaagcaat gccttctacc gtcgtaacct tgccgcaggt caaaaaggac tatctgtagc
    28801 ctttgatttg gctacacata gaggttacga ttccgaccac gaaagagtag ttggtgatgt
    28861 tggaaaagct ggtgtggcta tcgattcggt agaagacatg aaagttttat ttgaccaaat
    28921 tcctttagat gaaatgtcgg tttccatgac catgaacggt gcagtattgc ccatcatggc
    28981 attctatatt gtagctgccg aagaacaagg cgtggctcct cacctacttt cgggaactat
    29041 tcagaatgat attttaaaag agttcatggt gcgcaacact tacatttatc caccaacacc
    29101 atctatgaaa ataattgccg atatatttga atataccagc aaaaacatgc ctaaattcaa
    29161 ttccatttcc atttcgggtt atcacatgca agaagcaggt gcaacagctg atattgaatt
    29221 ggcatacact ttggcagatg gtttagaata catccgaaca ggattatcca ctggaatgaa
    29281 cattgacgaa tttgcaccgc gcctatcttt cttttgggct attgggatga accattttat
    29341 ggaaattgcc aagatgcgtg ccggtcgaat gctttgggca aaactcctaa aacaatttga
    29401 cccaaaagat gataaatctt tagcacttag aacccattgt caaacatcgg gttggagttt
    29461 aaccgaacaa gatcctttta ataacgtagc gcgaacaact attgaagcgg ctgcagctgc
    29521 ttttggtgga acccaatcgc tacacaccaa tgccttagac gaagccattg cgttgccaac
    29581 cgatttctcg gcgcgcattg ctcgtaacac acaaatatac ctgcaagaag agaccaaaat
    29641 ctgcaaaacc gtcgatcctt gggcaggcag ttattatgta gaaagcctta cggctcaaat
    29701 tgctgaaaaa gcttgggttc taattgaaga agttgaagaa cttggcggca tgaccaaagc
    29761 cattgaagct ggaattccaa aactgcgtat cgaagaagcg gctgctcgaa aacaagcgcg
    29821 aatcgatagc ggacaagaca tcattgtggg tgtaaataaa taccgcttag acaaagaaga
    29881 tccgcttcac attttagaag tagataatca aacggtgcgc aaacaacaaa ttgaacagtt
    29941 agaacacatc aaagtaacgc gcgatactgc caaagtaaaa gcgagtttag aaaaattaac
    30001 cgattgtgcc aagaatcaaa acgataattt attatcttta gcagtagaag ctgcccgaaa
    30061 ccgagctaca ttaggagaaa ttagcgatgc actcgaaagc gtttacggca gatacaaagc
    30121 acaaataaga agtttcagcg gagtgtatag caaagagatt aaaaacgacg aaagctttga
    30181 aaaagcaaaa caactggccg atgccttcgc taagaaagaa ggtcgtcgtc cacgcatcat
    30241 gattgccaaa atgggacaag acggacatga ccgtggtgcc aaggtagtcg ctaccggcta
    30301 tgccgatgta ggttttgatg tagatattgg tcctttattc caaacaccac acgaagccgc
    30361 caagcaagcc gtagagaacg atgtacacat actgggagtg tcttctcttg ctgctggtca
    30421 taaaacgtta gtgccacagg taatcgaaga gttgaaaaaa tacggtcgcg aagacatcat
    30481 ggtcattgta ggtggtgtta ttccttctca agattatcaa tacctttttg atgcaggtgc
    30541 tgttgctgtt tttggacctg gtaccaagat aagcgatgcc gctattagca ttctagaagt
    30601 attgttagaa acataagaca cattacctac ttgttcctcg aacaaagaat attctaaaac
    30661 acctacctaa ccgttggtgt ttttttgtta acaaatttcc tttctaacta gtaacaacaa
    30721 ataagttttt cttaaattcg tactacaatg aaaaccaaaa tatcatt
//
LOCUS       DAOOBH010000014        29202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_2086_length_29202_cov_5.532165, whole genome shotgun sequence.
ACCESSION   DAOOBH010000014 DAOOBH010000000
VERSION     DAOOBH010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 29202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29202
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2086_length_29202_cov_5.532165"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..984
                     /gene="era"
                     /locus_tag="PK218_03145"
     CDS             100..984
                     /gene="era"
                     /locus_tag="PK218_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPW97535.1"
                     /translation="MEHKAGFVNIIGNPNVGKSTLMNAFVGERLSIITSKAQTTRHRI
                     LGIVNGDDFQVVLSDTPGIIKPAYEMQKSMMDFVKSAFEDADVLIYMVEIGEKELKDE
                     AFFNKIIYSKIPVLLLLNKIDKSNQEQLEEQIALWKEKVPNAEIYPISALENFNVKEV
                     FARILELLPVSPPYYPKDALTDKPERFFVNETIREKILINYDKEIPYAVEIDTEEFIE
                     DEKIIRIRSVIMVERDTQKGIIIGHKGAALKKVGMQAREDLEKFFGKQIHIELFVKVN
                     KDWRSNAYQLKRFGYNQK"
     gene            complement(988..1482)
                     /locus_tag="PK218_03150"
     CDS             complement(988..1482)
                     /locus_tag="PK218_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97536.1"
                     /translation="METENEIRLRLRFYKDVNEPIAVIKEKLQAHRDTQPQDYSVKVN
                     DEHVWFHFKGDRKHYWSPHLHLELESKEDNTTHIRGLFGPDSTLWTLFVFLHFVVAGI
                     FIIFGMIAYSNYTLKAPLMMDVIIMFIMVIVWFLLYVIARQIREKGHDQMNELEQVFL
                     KIIR"
     gene            1574..2884
                     /gene="der"
                     /locus_tag="PK218_03155"
     CDS             1574..2884
                     /gene="der"
                     /locus_tag="PK218_03155"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPW97537.1"
                     /translation="MNNIVAIVGRPNVGKSTFFNRLIQRREAIVDSVSGVTRDRNYGK
                     SEWNGKEFSVIDTGGYIKGSDDVFEGEIRRQVELAIDEADAIIFVVDVEEGITPMDAE
                     VAKLLRKVTKPILIAVNKVDNAMREKDAVEFYNLGLGEYFTIASISGSGTGDLLDKLV
                     EVLPELPEVIEEENPLPRFCVVGRPNAGKSSYINALIGEDRFVVTDIAGTTRDAIDTK
                     YNRFGFEFNLVDTAGIRRKAKVKEDLEFYSVMRSVRAIEHSDVCILIIDATRGFEGQD
                     QSIFWLAEKNRKGIIILVNKWDLVEKDTMSTRDYERKIREELQPFTDVPILFVSALTK
                     QRLLKSLEATVQVFENRKQRIPTSKFNDYMLKLIEAYPPPALKGKYVKIKYCMQLPTP
                     TPQFVFFANLPQYVKEPYKRFLENKIRENWDFSGVPIDIYIREK"
     gene            2984..5764
                     /locus_tag="PK218_03160"
     CDS             2984..5764
                     /locus_tag="PK218_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPW97538.1"
                     /translation="MPKSFLWMLLFLGTSISFSQNTISIKGRVIDKNTKIPLESVTVY
                     LTSKIDSTVVDYTITNKMGFFDLKLKKTNKPLVLKVSYISYQDFKEDVSNLTIDKDFE
                     TIELEETTKSLDEVVVKSEAPPIRIKKDTLEFNASSFKVGADANVEALLKQLPGVEIT
                     PEGKITVNGKEVNNILVNGKPFFGKDGKIATQNLPAEIIDKVQVVDTKTKSEELSGEG
                     ASSEDKTINLTIQEDKNKGFFGKVMAGRGSDKRYESSLLLNYFKGEQKISVLASSNNI
                     NSVGFSMNEIFDNMGGGRNNSVYYDDDGRFGINGMQFGGGNGITQSNLIGINYQDKFL
                     KKVDNATSYYFSNAENENINRTNRINLLPAGTTFTNSNSSSRSVSNGHNFNTDFEIKI
                     DSTATIYINPTFSKNKSSSAFSRNQVTTNETFNELNSSTNDDYSENDNSSFATEASFN
                     KQFKKKLRGMSINFNAEINNQDQFTNTNSATLFADTTPDDIRQQNRFEKNKENKTRLE
                     IRYGEPITDSLRLSLTSEISFNKIDHRTKTFNFDAGSNDYTDFNDLQSNSILSTELSY
                     FPSTGVIIGKNKTNGRINFGPEFVNYKTESNYLGAVTNVNKNYVFPKIKGYISHRIGK
                     SKSIYANYNYEVTVPRASELLPVEDLSNPLNTIVGNENLEPTKRHNMYFGFNNYDYAS
                     RSGYYIYSGIDARKDAIVSSTVFDDNFKSTTTYENVDKTFSSYFGGNWSKSFKKEKRK
                     LSFSAGISFNYNINQGLTNAILFESKELEVNPRVNLTWSIDELITIAPSYKYSFSKTN
                     FENYVIDKADNFTHNAKIEITSYFPKKLVIGNDFGYTYNSNIADGFKKDFYLWNTSIG
                     YNFFNDKLLAKVKVYDLLNQNLNTRRSITPTAIIDSENTVLRRYVMFSLTYKLEKFGG
                     KKKNENMMFFED"
     gene            complement(5962..6765)
                     /locus_tag="PK218_03165"
     CDS             complement(5962..6765)
                     /locus_tag="PK218_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPW97539.1"
                     /translation="MKIPNSKNLVSFLTVLFLVVTQVVFAQFNIPEKPSFQTSVYDYA
                     NMLSGSEKQQLEEKLIRYSDSTTTQIVVITIESLKGSDIGILTPEWGQKWGIGGTKKD
                     DNGVIVVLAKAERKIWISAGYGVEDRLSAGIIGELTRNVIIPEFKAGSYFNGLDKGTT
                     AIIEVLKGKYKGERKQNKKGDFPILPIIIVIIIIIVIFSKNKNNGGNSGNRGGGMSLT
                     DMIILSSLGRNSGGFGGSSGGGFGSSSGGGFGGGFGGGGFSGGGAGGSW"
     gene            complement(6783..7220)
                     /locus_tag="PK218_03170"
     CDS             complement(6783..7220)
                     /locus_tag="PK218_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPW97540.1"
                     /translation="MSKVEDFLTQAEEQEIVAAIGLAEKNTSGEIRVHIEKKTSIDAF
                     DRAMEVFHLLKMDETELRNGVLIYVAVKDKTFVIYGDKGINEKVADDFWNSTRDVMVS
                     HFKNGNFKQGLIDGIIKAGEELKKFFPYQDDDINELSNEISKG"
     gene            complement(7224..7832)
                     /locus_tag="PK218_03175"
     CDS             complement(7224..7832)
                     /locus_tag="PK218_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPW97541.1"
                     /translation="MNFKKFLPWIIVIVILFIGYNYVKGINNKAVVFEQDIKEAWGNV
                     NTAYQRRNDLIGNLVKTVKGAADFEKSTLEAVVNARAKATSVTVDPTNITPEQLSQFN
                     QAQGGLNSALSRLLVTVEKYPDLKANQNFLKLQDELASTENQILTARTRFNEATKPYN
                     SHIKVFPNNLFAGMFGFKEKPYFEAVAGAEKPVDVEFDFDKK"
     gene            complement(7855..8187)
                     /locus_tag="PK218_03180"
     CDS             complement(7855..8187)
                     /locus_tag="PK218_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPW97542.1"
                     /translation="MHLELKEGKLYYSIGELAKAFNVNASLIRFWDSEFDVLKPKKNA
                     KGNRMFTPEDVKNLQLIYHLVKERGFTLEGAKTHLKEGKKETLTNFDIITKLETIKAQ
                     LTDLKNNL"
     gene            complement(8233..9213)
                     /locus_tag="PK218_03185"
     CDS             complement(8233..9213)
                     /locus_tag="PK218_03185"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW97543.1"
                     /translation="MAKKVKYYFDTESLAYKKIKTSVKKKFAYVGGFILSSALFGFLC
                     FVVLLNSTFFETPKDRLLTREIETMKLKYSILNKKMDQIDEVLVNIEERDNNIYRAYF
                     NTSPIPNEQRKAGFGGVNRYKELAGLNNADLVINTSKRVDIISKELAIQSKSLDEILK
                     LAKDKNKLLAAIPAIQPVKNEQMKRVASGFGYRSDPFTKARKMHEGMDFTANTGTPIY
                     ATGDGVVINADNSKSGYGNHIEINHGFGYLTLYGHLSKYNCRAGQRVKRGDIIGYVGS
                     TGRSEAPHLHYEVHKDGKVVNPLNFYYGNISAEEYTVISKLANQENQSLD"
     gene            9415..12051
                     /gene="alaS"
                     /locus_tag="PK218_03190"
     CDS             9415..12051
                     /gene="alaS"
                     /locus_tag="PK218_03190"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPW97544.1"
                     /translation="MKSQDIRKAYLQFFESKNHLIVPSAPIVLKDDPTLMFNNSGMAQ
                     FKEYFLGNGTPKSSRIADTQKCLRVSGKHNDLEDVGFDTYHHTMFEMLGNWSFGDYFK
                     KEALAWAWEFLTEVLKLDKDRLYVSVFEGNPSENVPFDQEAFDIWKQYVPEDRIILGN
                     KKDNFWEMGDQGPCGPCSEIHIDLRTDAERAAVSGRSLVNADHPQVVEIWNNVFMEFN
                     RKADGSLEKLPAQHVDTGMGFERLCMAMQNVTSNYDTDVFTPLIAKIEQITRLKYTSN
                     EVKNISEEQNKINIAIRVIVDHARAVAFAIADGQLPSNTGAGYVIRRILRRAIRYGFT
                     FLDTKEPFIYKLVEVLAHQMGEFFPEIKSQQNLVTNVIREEEASFLRTLEQGLQLLDK
                     VVAETSGKEVSGAKVFELYDTFGFPKDLTALILKEKGYSYNEEDFEAELQKQKARSRA
                     ASEVSTEDWSVLIPGNVESFVGYDQTENEVKITRIRKVDSKKDGVLYQIVLDATPFYP
                     EGGGQVGDKGTLVSANETIEIIDTKKENNLILHFAKQLPENVEASFVAKVNTALRTST
                     SKNHSATHLMHLALRTILGTHVEQKGSLVNPNYLRFDFSHFSKVTDEELKQVEDFVNQ
                     RIAEQLQLNEYRNLPIQEALAKGAMALFGEKYGDSVRMIEFGESKELCGGIHVKNTAD
                     IWHFKIISEGAVAAGIRRIEAITGDAVKEFYTNQENTLAELKETLKNPQDVLKAVNSL
                     QDDNAKLKKQVEQLIKDKAKGLKGELASQIQEVNGVKFLATQVDLDANGAKDLAYDLG
                     ANETNLFIVFATVTEDKPMLTCYISKELVSDKGLNAGQVVRELGKYIQGGGGGQPFFA
                     TAGGKNAAGIPEALEKAVDFVN"
     gene            12418..13227
                     /locus_tag="PK218_03195"
     CDS             12418..13227
                     /locus_tag="PK218_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97545.1"
                     /translation="MKPTFKQIIFLVFFFVGIQFSISATSNPTTPQNETILLKKLQSK
                     VLGNDSEYLFSYNDKKITKIDYTTFDGVTRKGYFKFTYSGDLITKIQEFTTENVNLFT
                     STFTYTNGQLAGVVTEEIGKNKAEKINFIHNGTASVTAVRSIGTSSQQNNIVENEVMY
                     FTDNKLVKKVENGVVSSSTIYEYDRANNPLQNVIGMDKINVYLNTSFGIFSSSGLEGI
                     QNNRVKQTVYTSKGTLEYVLVFNTTYTDVNFPNTRMSPPNSPGAFEYNYKY"
     gene            13442..14845
                     /locus_tag="PK218_03200"
     CDS             13442..14845
                     /locus_tag="PK218_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPW97546.1"
                     /translation="MRNFLILLTICSFNFFSGQNNRLPDGGGFYDFNHQNTECLSEEQ
                     RLTIKGLLDENIKHLTLESKINLNKSSVFNPTNHPLFIWPVVKSVTAPYNNVWSISNY
                     VDHNPNYPNAVQDWNCGSRTYDTAAGYNHKGIDIFTWPFSLFQMDNDQAEAVAAADGI
                     IILKSDGNFDRSCAFNNNNWNAVYIQHADGSQSWYGHLKNGSLTTKVVGDFIAAGEYV
                     GIVGSSGNSTGPHLHFEVYNNSGQLVDTYLGPCNNWSSSTETWWENQKPYLDPKINAV
                     LSHSAPPQFNTCPTTETPNISSSFSAGSNVVLAIYLADQLANSSVNLVLKRPDNSIQA
                     NWNFSLVNEYSASWWYWIFPSNQMIQQGNYQFTATYQGSSVTHDFNYGNLGIDESLAS
                     KFSITPNPVQESFSINGSDTIIIESVTIYDIVGKKVIQTNSSSKDINIAALDNGVYIV
                     NVMTLEGSYTAKIIKKQ"
     gene            complement(14856..16598)
                     /locus_tag="PK218_03205"
     CDS             complement(14856..16598)
                     /locus_tag="PK218_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW97547.1"
                     /translation="MKLLYSYILKHKLLLFFALLMATINICFSLSDSIITGKLLQDCG
                     VNIANYAGNKVGFLKSLGFWLGLSLGAAMISRITKNFQDYFTNIVIQRTGAEMYTDGI
                     KKSLDLPFEDFEDQRSGETLSKLSKVRTDSEKLITLSISLIFQTIIGFVFVILYVSRI
                     DARISLIFLVTAPIIALVSSYLGKKIKVVSRKIVNQTNSLAGSTTESLRNIELVKSLG
                     LTHQEEKRLNLNTLRILQLEIQKVRFIRSLSFIQGTTVHFLRTCVVFTLYYFLFGGKI
                     IVGDLLTMVFFTFFIFGPLQELGNFIITLNETKVSMENFKTLLSAPKEFRPANPKNIK
                     KITTLKFSNVSFKHKTATQKAVENINFDIKQGETVAFVGPSGSGKTTLVKLLVGLYPP
                     AEGKVLYNDIDSKEIDLLDLRKQLGFVTQDAQLFSGTIRENLLFVKPNASDEELYEVM
                     QKASCQKLLDRAEDGLNSTIGEGGIKVSGGEKQRLSIARAILRNPNLLIFDEATSALD
                     SITEEEINETIRTVSDQNRITILIAHRLSTVMHADKIFVLEQGKIIEEGKHDDLVAEK
                     GLYYAMWRQQIGER"
     gene            16714..17661
                     /locus_tag="PK218_03210"
     CDS             16714..17661
                     /locus_tag="PK218_03210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HPW97548.1"
                     /translation="MDFRTIIPIKKSQNPIDYNSQLLLMGSCFAENMGEKLEYFKFQT
                     TVNPFGIIFNPVSIEKLVDRMVNQKPFTDQDVFLHNDLWHCYEVHSGLSNPDKEDFLK
                     SLNHTIDQSHQQITKVTHFQITYGTSWVYRHKTSNEIVANCHKVPQKEFEKELLSVEQ
                     IQSSIQNTIALVQKINPDCHFIFTISPVRHIKDGFIENQQSKAHLVTALQSSIFHLPT
                     SSYFPSYEIMMDELRDYRFYAEDMLHPSQVAIDYIWVRFFENYVSETEFEIMNEICTI
                     QKSLSHKPFNPESESHQKFLVSLEQKILKIKEKLPQVQF"
     gene            17740..19599
                     /locus_tag="PK218_03215"
     CDS             17740..19599
                     /locus_tag="PK218_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPW97549.1"
                     /translation="MKIFLKTTKVICFFTIMVFNSSFAQTTPTNTYTIDGKINFMKLT
                     DTGILLIAHNDGFAGIKPESNKLIFDFKDYGSVKEEELQFIPATPYVVISQGGFAKLS
                     SKKIVFDYVVGKQLFETKENGWKDAFSAQVFIPQNKLIVSGIRTSKEKFAMAVGIYDL
                     VTGKEEKLIYPVEPGKVTMGSVSVTGSIFMKDNLVYIPTSKELICYDIKSNSRVWSAK
                     VDDLSWMTADESGKEIYGFENTNSGDTKINKISSTGEVLWKDSQKVKGAVTRFEILPQ
                     GIAIVSDVADKGGSVFSARAESKIMMLSAKDGEDLWDKAPKTKGYVQHFYVMDDGILF
                     GIQSGGINKISFTGETLFKKPLKTGENIHTMALTPKGMIYITDSDANIIDLKTGESIW
                     NKPIKYKNASAVASTYDAKNKRYLISTGDDVVAIDENSGDVKTLSEFKFEEKEAPNSF
                     SVRANGLLLTSDQNIMMLNFDGTKQFHEYYKSPGRSTFGKIMGGVMAVASTAASVSMA
                     ARAGANKNSIGQYNEYGREADRASKMFADIGSASFDYMSKRFKASAATANAQFILTKL
                     DDGVGLVKINKDSGAKEKEIVLKDKKPEYEVDEYAGVLYYKKDNNTILSYDLK"
     gene            19662..20195
                     /locus_tag="PK218_03220"
     CDS             19662..20195
                     /locus_tag="PK218_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97550.1"
                     /translation="MKTIKQIGLILFATIAVSLSSCSKSDGGSGGDAGSGTMKAKVNG
                     TSVTTLEITTFATISSNVLILQGNTGGTSSKAFYINVGSYNGVGTYDIGGGTTGFGQA
                     TASYMEILVDPSNPTAFESTTWQAPYEGGAKVGEVSVSEVTSSNIKGTFHYMAKKNTG
                     DGALIEITEGSFNIPLD"
     gene            20668..22554
                     /locus_tag="PK218_03225"
     CDS             20668..22554
                     /locus_tag="PK218_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPW97551.1"
                     /translation="MRCFYFLFFIFLFQNTFNAQSLHETLGKAFTSSDSSDYYFKIAK
                     KSIKTKADEGQYYFCKNARCTDYNQFDSAVVYGERAIKLLQRKEDLNSLLTVYLNFNK
                     LYNNQGQYEKAIAYSLKGLKIAEKEKNEAWIIEYFINLSVDYHDYESFEKGIDYGKKA
                     LLKLKKKKQTNPGSLWRAYNSIAINFDDWNQPDSALHYHKKALSFFKGSDTLKIPTTF
                     NNIGNTLLKQKKYKEAKRWLTSGLKISEISDKKEDIYFFYGRATLYTNLAIIESELGN
                     YQQAENWFSKAHQYAVKSNSAEKLRDIYAARAKFYTKTKNYQKAVADHEEYIKIRDSI
                     FDAERDKTFSELEVKYQSEKKEKEILQAKAKLFKSQTEIKSKNTQFLILSLITIGLIV
                     ISLLIYRQQKLKRNQQQQEFELKSAIAKIETQNKLQEQRLQISRDLHDNIGSQLTFII
                     SSVDNVKYAFNINNDKLDNKLSGISDFAKATIIELRDTIWAMNKSEITIEDLESRIHN
                     FVEKAKIAKTEIAFNFHVDDRLKEVKFTSVQGMNIYRTIQEAINNCIKYAEAKSISIE
                     INQTNDTIEIIIQDDGKGFNQNQVSFGNGIENMKKRVKDINGEIKFNSIIDKGTTIII
                     RLKK"
     gene            22559..24496
                     /locus_tag="PK218_03230"
     CDS             22559..24496
                     /locus_tag="PK218_03230"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPW97552.1"
                     /translation="MNKVYFTLLLLFLKGTFGFTQNALELITKLKTELKTNPDDKRKA
                     TIYSDIAWYYVNVSTDSALVYSKKAMNESTKLNDSVLIAQVNSDLGAIYFRRNDFENA
                     KKYYGVALNIRKARKDNVGIAKINLHMANIYNKENKKEKALQYYLAGIDYFEKTNNPE
                     IVANTKGNVAALFFELKNYPKAKKYLNDAIKYQEAKKDDNGLSTSYLTLGNIYLKTND
                     TLNALKLYEKSIASAKKSANNVALSSAMNNLGVIKSEQKKSSEAQTLFANSKAVKDSL
                     SVKKDASISDLSIAKEHIMYQRFDQANKVLYQLKERYQNNPSEQELLLSTYQFFIHTY
                     AYLHQPDSVNYYNNLATNIQSKIVETAIAKQTNELETKYQTAKKEKQLLLKEIEIKQT
                     RDRLFLVIGIALIIALIGWLIYRQQKIRNKQQQQEFELKSAIAKIETQNKLQEQRLQI
                     SRDLHDNIGSQLTFIISSVDNVKYAFNINNDKLDNKLSGISNFARETIIELRDTIWAM
                     NKSQITLEDLESRIHNFVEKAKIVKTEIAFTFKVDERLIDIQFSSVEGMNIYRAIQEA
                     INNSIKYANATAISIDMFKENDTIKVTINDNGIGFSSDEVNLGNGIVNMQKRIKDIGG
                     TFEMNAQKGIGTTIIFKVPMP"
     gene            24865..25512
                     /locus_tag="PK218_03235"
     CDS             24865..25512
                     /locus_tag="PK218_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW97553.1"
                     /translation="MSTRIAIVDDNVFLQKAVAEKLSFFDDLTLKYTAINGNDLLAKL
                     EKDKNLDLILMDIEMPECNGIEATKIVKAKYPQLKIIMLTVFDNDENIFNAIKAGADG
                     YLLKEVNPKELHQGIIETLNGGAAMNPSIALKTLKLLRNPVDFEAKEQEDIKLTTREV
                     EVLEQLSKGLNYNQIAENLFLSPSTVRKHIENIYSKLQVHSKIEAVQKAKDNNII"
     gene            25615..27075
                     /locus_tag="PK218_03240"
     CDS             25615..27075
                     /locus_tag="PK218_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPW97554.1"
                     /translation="MNAKKLFSKWSLGLLLFGFGLVSNANNLQISGTAVSGSNISFNI
                     SWENSWNTNIAPANWDAVWVFVKYQDCATRQWYHAAIATTGNTAASPLQVDLVPDAKG
                     VFLRRSAFGGGDIASTAITLALNIPAGTYNYKVFGIEMVNIPQGDFQVGDATSTGTYS
                     SATITASSQSGGLSAATLGGASVNIPATFPMGYNSFYSMKYEVTQEQYVEFLNTLTYD
                     QQKERTGIDPIAAPGTGAFTTGSQYRNGVTLITSGNNSTLPAVFACDATAGVENNVDD
                     GQNIAMNWMSWADLAAYLDWACLRPMTELEFEKICRGTAPRVAGEYPWGTTDFNYYST
                     GSIVGGTGFKSNENISTIVNGRNLWSNSGVVAGAGYGPCRVGMFATSVTGRSSSGAAY
                     YGVMEMAGNVWERTVTTGNATGAAFTGALGDGTLTVLGDADVATWPSSTTALGLGLRG
                     NGYYNTSANPSRTSDRTNATTASPTRSYTYGGRGVR"
     gene            27147..>29202
                     /locus_tag="PK218_03245"
     CDS             27147..>29202
                     /locus_tag="PK218_03245"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPW97555.1"
                     /translation="MKTTYIKKVVFILFLLSSSFIYSQYNGGDSDGFSSSSLNNTSCS
                     YPTQFIAYNGGDSSLTTINSLINTTCNAPNQFFAYNGGNGDGFAFESINAVSCANPNQ
                     FYAYFGGNGDGFANQAINAISCPNPSQFYAYFGGNADGFSVGSTAPACPTAPPVADFT
                     ASATTICVGQSVTFTDTSTNSPSAWTWTFTGGTPNSSTVQNPVVVYNSPGTYTVTLLA
                     ANFNGNDTETKTAYITVNAIPTVTTTTPASRCDSGTVTLQATASSGATLNWYAAATGG
                     TALSSGTSFTTPSLTATNTYYVEAQFGTCISARTAVIATVNATPSITATTPASRCGTG
                     SVTLQATASAGTIQWFADAAGGTALFTGANYTTPSLTTTTSYFVQVSQNGCISPRTEV
                     VATINTTPTITGTTPATRCDAGTLTLQAVASAGTISWYATATGGTALATGTSFTTPSL
                     STTTTYYVGTATATCTSTRTAVIATVNATPSITGTTPGSVCDSGTVSLSASTSAGIIS
                     WYAVASGGTVLGTGTTFITPSLTATTTYYAEASNGGCTSARTAVIATVNTTPTITGTT
                     PASRCGNGSVTLQATANVGTIRWFGSATGGSVLATGSSYVTPNLNSSTTYYVEVTQNN
                     CTSPRTAVLATINTIPTITGTTPANRCGDGSVTLQATASSGTISWYATATGGTALATG
                     GSF"
BASE COUNT        10463 a         5023 c         4973 g         8743 t
ORIGIN      
        1 gtcacaggtt cgagtcctgt tcccgctact aagaataagc cattcagcaa tgagtggctt
       61 tttttgttac ctttgcaaaa agaaataacc atacaaatca tggaacacaa agcaggtttt
      121 gtaaacatca tcggaaaccc aaacgtaggc aaatcaaccc taatgaacgc tttcgttggc
      181 gaaagacttt ccattataac ctcaaaagca caaacaacgc gacacagaat actaggaata
      241 gtaaacggtg acgatttcca agtagtcctt tcagatacac ccggaatcat caaaccagcc
      301 tacgaaatgc aaaaatcaat gatggatttc gtaaaatctg ccttcgaaga cgccgatgta
      361 ctaatataca tggtcgaaat aggagagaag gaactaaaag acgaagcatt tttcaataaa
      421 attatatact ccaaaatccc tgttttatta ttgttgaaca aaatcgacaa atcaaaccaa
      481 gaacaactcg aagaacaaat cgctctttgg aaagaaaaag taccaaacgc cgaaatctat
      541 ccaatatctg ccctagaaaa ctttaacgtc aaagaagtat tcgcccgaat attagaatta
      601 cttccagttt caccaccata ttacccaaaa gacgcactaa ccgacaaacc agagcgcttc
      661 ttcgtaaacg aaaccatccg cgaaaaaatc ctaatcaact acgacaaaga aataccttat
      721 gccgtagaaa ttgacaccga agaattcatc gaagacgaaa aaataatccg aatccgttcc
      781 gtaataatgg tcgaacgtga tacccaaaaa ggaatcatca tcggacacaa aggcgcagca
      841 ctcaaaaaag taggaatgca agcccgtgaa gatttagaaa aatttttcgg aaaacaaata
      901 cacatcgaat tatttgtaaa agtcaataaa gactggagaa gcaacgccta tcaattaaaa
      961 cgattcggat acaaccaaaa ataatattta gcgtattatt tttaaaaaaa cttgttccaa
     1021 ttcattcatt tggtcatgac ctttttctct aatttgtcgc gctataacat acagcaaaaa
     1081 ccaaacaatc accataataa acataataat aacatccatc attaaaggtg ctttcaaagt
     1141 ataattagag taggcaatca ttccaaaaat gataaatatt cctgccacca caaaatgaag
     1201 aaacacaaac aacgtccaaa gcgtagaatc tggaccaaac aaacctcgaa tatgtgtcgt
     1261 gttgtcttct tttgattcta attcaagatg aagatgtgga gaccaatagt gttttctatc
     1321 acctttaaag tgaaaccaca catgttcgtc attaactttt actgaataat cctgaggttg
     1381 agtatcacga tgcgcttgta atttttcttt aataacagca ataggttcgt ttacatcttt
     1441 gtaaaaacga agtcgtaatc taatttcatt ttcagtttcc atttgaaaaa taggttggtt
     1501 taatagacta aagtaataaa aaaattgata ttgttttcat aaatagtacc tttgcaccaa
     1561 tatttaaaat aacatgaata atatagtagc catagtagga agaccaaacg tagggaaatc
     1621 tacttttttc aatcgtttaa tccaaagaag agaagccatt gtcgactcag tttctggagt
     1681 aactcgtgat agaaattatg gcaaaagtga atggaacgga aaagaattct cagtaatcga
     1741 tactggcgga tatatcaaag gttctgatga tgtttttgaa ggcgaaatac gtcgtcaggt
     1801 agaattagca attgacgaag cagatgccat catttttgta gtcgatgtcg aagaaggaat
     1861 tacaccaatg gatgccgaag ttgcaaagct tttaagaaaa gtaaccaagc caattctaat
     1921 cgccgtcaac aaagttgaca acgccatgcg cgaaaaagat gcagtcgagt tttataacct
     1981 aggattagga gaatacttca caatcgccag tattagtggc agcggaacag gtgatttgct
     2041 agacaaacta gtagaagtgt taccagaact tccagaagta atagaagaag aaaatccttt
     2101 accacgtttt tgtgttgtgg gtcgtccaaa cgcaggaaaa tcatcctata tcaatgcatt
     2161 aattggcgaa gacagattcg tagttaccga tatagctgga acaactcgtg atgctatcga
     2221 caccaaatac aatcgttttg gtttcgaatt caacctagtt gataccgccg gaattcgtag
     2281 aaaagccaaa gtaaaagaag atttagaatt ttattccgtg atgcgttccg ttcgtgcaat
     2341 agaacattca gatgtatgta ttttaatcat cgatgctact cgaggcttcg aaggacaaga
     2401 ccaaagcatt ttttggttag ccgaaaaaaa cagaaaagga ataatcattt tagtaaacaa
     2461 atgggattta gtagaaaaag atactatgtc aacccgcgat tatgaacgta aaattcgtga
     2521 agaattacaa ccatttaccg atgtaccaat tcttttcgtc tcggccttaa caaagcaacg
     2581 tttattaaaa tcattagaag ccacagttca agtttttgaa aacagaaaac aacgcatacc
     2641 aacctcaaag ttcaacgatt atatgttgaa acttattgaa gcctatccgc cgccagcatt
     2701 aaaaggcaaa tatgtaaaaa taaaatactg tatgcaattg ccaacaccaa caccacagtt
     2761 tgtgttcttc gccaatttac ctcaatatgt aaaagaacca tacaaacgtt tccttgaaaa
     2821 taaaatccgc gaaaactggg atttttcagg tgtgccaatt gatatttaca ttcgcgaaaa
     2881 gtaaaaaatt tgttaaatta tcgtaagatt ttttaacatt acacaataaa ataagtactt
     2941 agtcgtttat agttatacct aaccttaaac gactattttt tttatgccaa aatcctttct
     3001 ttggatgcta ttgtttttag gcactagcat ctctttttcc caaaacacta tttctataaa
     3061 aggaagagta atcgacaaaa acaccaagat tccactcgaa tcagtaacgg tttacctaac
     3121 ttcaaaaatt gattcaaccg tggtagatta caccattacc aacaaaatgg gattttttga
     3181 tttgaaacta aaaaagacta acaaaccttt agtacttaaa gtttcttata taagttatca
     3241 agattttaaa gaagatgttt ctaatttaac aatcgataaa gactttgaaa caatcgaatt
     3301 agaagaaacc acaaaatcat tagacgaagt tgttgtaaag tcagaagcac caccaatccg
     3361 gattaaaaaa gacacactcg agtttaatgc ttcgtctttc aaagtaggtg ccgatgccaa
     3421 tgtagaagct ttattaaaac aactacccgg tgtcgaaata actcccgaag gaaaaattac
     3481 cgtcaacgga aaagaagtca ataatatttt agtaaatgga aaaccattct ttggtaaaga
     3541 cggaaaaatt gccactcaaa atcttcctgc cgaaatcatt gataaagtac aggtagtaga
     3601 taccaaaacc aagtccgaag aactctctgg cgaaggcgca agctcagaag acaaaaccat
     3661 aaatcttacc attcaagaag ataaaaacaa aggttttttt ggtaaagtga tggcaggacg
     3721 aggatcagac aaacgatatg aatccagttt gttgctcaat tattttaaag gcgaacaaaa
     3781 gattagcgtc ttggcatctt ctaataatat aaattcagtg ggattttcca tgaatgaaat
     3841 atttgacaat atgggtggag gaagaaataa ttctgtgtat tatgacgatg acggaagatt
     3901 tggcatcaac gggatgcaat ttggtggtgg aaatggcatt actcaatcca atctaattgg
     3961 aattaactat caagacaaat tcctgaagaa agtagataat gctacaagtt attatttttc
     4021 aaatgcagaa aacgagaaca ttaaccgaac caatagaatt aacctattac ctgctggaac
     4081 cacatttacc aattcaaatt cttcttcaag aagtgtgagt aacggtcata attttaatac
     4141 tgattttgaa attaaaattg attcaacagc aacaatttat atcaacccaa cattttctaa
     4201 aaataaaagc agtagtgctt tttctagaaa tcaagtgact actaatgaaa cttttaatga
     4261 gttaaatagc agcactaatg atgattattc tgaaaatgac aactccagtt ttgcaacaga
     4321 agcaagtttc aacaaacaat ttaaaaagaa actacgaggc atgtcaatta attttaatgc
     4381 cgagattaat aaccaagacc agtttaccaa caccaattca gcaacacttt ttgccgacac
     4441 aacacccgac gatataagac aacaaaaccg atttgagaag aataaagaaa acaaaacccg
     4501 tttagaaatt agatatggcg aaccaataac cgattcactt cgattgagct tgacttccga
     4561 aattagtttt aataaaatag accatagaac aaaaactttc aattttgatg cgggtagcaa
     4621 tgactatact gattttaatg atttgcaatc caactccatt ctatcaacag agttaagtta
     4681 tttcccatca acaggtgtaa ttattggtaa aaacaaaacc aacggtagaa ttaattttgg
     4741 tcctgaattt gtgaattaca aaacagaatc caattattta ggagcagtaa cgaatgtcaa
     4801 taaaaactat gttttcccaa aaataaaagg atatatcagt catcgaatag ggaaatcaaa
     4861 atcaatatat gccaattata attatgaagt taccgttcct agagcgtcgg agcttttgcc
     4921 tgtggaagat ttatcaaacc cattaaatac tatagttgga aatgaaaacc tagagccaac
     4981 caaacgacat aatatgtatt ttggatttaa taattatgat tatgcatcac gttcgggtta
     5041 ttatatttat tctggaatag atgctagaaa agatgctatt gtttcttcta cagtttttga
     5101 tgataatttt aaatctacta caacctatga aaatgtagat aaaacctttt catcctattt
     5161 cggaggaaat tggagtaaat cgttcaagaa agagaaacga aaattaagtt tttcagcagg
     5221 aataagtttt aattacaata tcaatcaagg gttaacaaac gccattttat ttgaatcaaa
     5281 agaattggaa gtcaatccgc gagtgaattt gacttggagt attgatgagt taatcacgat
     5341 agcaccaagt tataaatatt cattttcaaa aaccaatttt gagaattatg taattgataa
     5401 agcagacaat tttacacata atgcgaagat tgaaatcact tcttattttc caaagaaact
     5461 agtcatcgga aatgattttg gttataccta caactccaac atagctgacg gatttaaaaa
     5521 agatttctat ctgtggaata ctagtattgg ttataatttt tttaatgaca aactacttgc
     5581 caaagtgaaa gtatatgatt tgttaaatca aaatctcaac acaagaagat cgattacacc
     5641 aaccgcaatc atcgactctg aaaatacagt tttaaggcgc tatgtaatgt tttcattaac
     5701 ctataaacta gaaaagtttg gcgggaagaa aaagaacgaa aacatgatgt tctttgaaga
     5761 ttaaacaaaa acctcgttca agttgaacga ggttttttat tatagtattc gacaaaaaaa
     5821 agcacctcaa atttcattaa tatttacaaa ttagtatgag ttgataaatg aaagttgctt
     5881 gttgatgttt tgttttttaa taaattgact gttgtgggtg atgaagattt aacttccaac
     5941 tttgtgcctc gtaattcgat gctaccaact accaccagca ccaccaccag aaaatccgcc
     6001 gccaccaaag ccaccgccga agccaccgcc tgatgaactg ccgaaaccac cgcctgatga
     6061 accaccaaaa ccaccactgt ttctacccaa actgcttaaa ataatcatgt cggttagact
     6121 catgccgcca ccacgattac cagaatttcc gccattattc ttgtttttag aaaagataac
     6181 tataattata atgattacaa taataatggg taaaatagga aaatcacctt ttttgttttg
     6241 ctttcgttcg cctttgtatt tcccttttaa tacttcaata atggccgttg tacctttatc
     6301 taaaccatta aaataactac ctgctttgaa ctcagggatg attacatttc tagtaagttc
     6361 gccaataatt ccagcactaa gtctatcttc tacgccataa ccagcagaaa tccatatttt
     6421 acgttcagct tttgctaaaa ctacaataac accattgtca tctttctttg taccaccaat
     6481 tccccatttt tgtccccatt ctggtgttag tattccaatg tctgagcctt ttaaactttc
     6541 aatggtaatg acaacaattt gggttgttgt agaatctgag tagcgtatta gtttttcttc
     6601 taattgttgt ttttcagagc cactaagcat gttggcatag tcatacacag aagtttgaaa
     6661 tgaaggtttt tcaggtatgt tgaattgtgc aaaaacaact tgggtaacta ctaaaaataa
     6721 aacggttaaa aacgatacta aatttttgga atttggaatt ttcattttgt ttttttagta
     6781 ggttatcctt ttgaaatttc gtttgataat tcatttatat catcatcttg ataagggaaa
     6841 aacttcttca attcttcacc agctttaatg atgccgtcaa ttaaaccttg tttgaaatta
     6901 ccatttttaa agtgagaaac cattacgtct cgggttgaat tccaaaaatc atcagctact
     6961 ttttcgttaa tgcctttatc gccatagatg acaaatgttt tgtcttttac ggccacataa
     7021 attaaaacac cattgcgcaa ctcagtttca tccatcttta ataaatgaaa aacttccata
     7081 gcacgatcaa acgcatctat ggaagttttc ttttctatat gaacacgtat ttcgcccgaa
     7141 gtattttttt cagctagacc aattgcggca acaatttctt gttcttctgc ttgggttaaa
     7201 aaatcttcta cttttgacat gttttatttt ttatcgaaat caaattctac gtcaactggt
     7261 ttttcagctc cagccacggc ttcgaaatat ggtttttctt taaaaccaaa cattcctgca
     7321 aatagattgt ttggaaatac tttgatatga ctattataag gttttgtagc ctcattaaat
     7381 cgtgttctag ccgttagaat ttgattctcg gtacttgcca attcatcttg taatttcaag
     7441 aaattttggt tggctttcaa atcaggatat ttctcaacag ttactaatag tcttgataat
     7501 gcactgttta atccaccttg cgcttgatta aattgactta actgctctgg tgtaatgttt
     7561 gttgggtcaa cagtaaccga agtagctttt gctcttgcat taaccacagc ttctaatgta
     7621 gatttttcaa aatcagcagc accttttacg gtttttacta aattcccaat aaggtcattt
     7681 ctgcgttgat aagctgtatt cacgttaccc caagcttctt taatgtcttg ctcaaaaact
     7741 acggctttgt tgtttattcc ttttacatag ttgtatccaa taaatagaat aacaatgaca
     7801 ataatccacg gtaaaaattt tttaaaattc atgttttaaa tatttaagtt aagtttataa
     7861 attgtttttt aaatcagtta gttgtgcttt gatagtttca agttttgtaa taatgtcaaa
     7921 attagtaagg gtttcttttt tgccttcttt taaatgtgtt tttgcacctt ctaacgtaaa
     7981 gccgcgttct ttcaccaaat gataaattaa ctgcaaattc tttacatcct ctggtgtaaa
     8041 catacgattg cctttggcat ttttcttggg tttaagtaca tcaaattcgc tatcccaaaa
     8101 acgtattaaa gaggcattaa cattaaatgc ttttgccagc tcgccgatac tgtaatataa
     8161 ttttccttct tttagttcta aatgcatagt ttttttatga ttctttattt agcaatctaa
     8221 tagttacaat agttaatcta aagattgatt ttcttggtta gctaattttg aaataacggt
     8281 atattcttcg gctgaaatgt tgccataata gaaatttaaa ggatttacta ctttaccatc
     8341 tttatgtact tcgtagtgta aatgaggtgc ttcgcttctt ccagtactac ctacatatcc
     8401 aatgatgtca cctcgtttta cacgttgtcc agctctacaa ttgtatttgc tcaaatgacc
     8461 ataaagcgtc aggtagccaa aaccatgatt aatttcaata tggtttccat agcccgactt
     8521 tgaattatca gcattgatta ctacaccatc accagttgcg tatatagggg ttcctgtatt
     8581 tgccgtaaaa tccatgcctt catgcatttt tcttgctttg gtaaacgggt cgcttcggta
     8641 tccaaaacct gaagcaacgc gtttcatttg ttcattttta accggttgta ttgctggaat
     8701 tgcagctaac aacttgtttt tgtcttttgc tagctttaaa atttcatcca atgatttcga
     8761 ttgaatagcc agttcttttg agatgatatc cacgcgtttt gaggtattaa tcaccaaatc
     8821 cgcattgttt aatcctgcta attctttata ccgattgacg ccaccaaaac ctgcttttcg
     8881 ttgctcgttt ggaataggcg atgtgttaaa atacgctcta taaatattat tatctctttc
     8941 ttctatgttg accaaaactt catcaatctg atccattttc ttgttcaaaa tagaatattt
     9001 caacttcatt gtttcaatct cacgagttag taatcggtct tttggtgttt caaaaaaagt
     9061 agaatttaat aaaactacaa aacacaaaaa accaaacaac gcagaagaca aaataaatcc
     9121 accaacatag gcgaactttt tcttcacaga agtctttatt tttttataag ctaaactttc
     9181 ggtatcgaaa taatacttta ctttcttcgc cattttttat tttatgctat ttttgtagcg
     9241 tttaagagaa acactagtta tacgaacaaa tttaacaaat gtttcaatga aatactaaat
     9301 taattttttg gaagtagtat ttctaacatt tcgagcaact tagacatcca aaataaagtg
     9361 agtgttgatg gttttttaat caaaagctat caaccaaaaa ttataaatag aacaatgaaa
     9421 tcacaagaca ttagaaaagc gtatttgcag ttctttgaaa gcaaaaacca tttaattgta
     9481 ccatcggcac caattgtcct taaagacgac ccaaccttga tgttcaacaa ttctggaatg
     9541 gcccaattca aagaatattt cttaggtaac ggaactccta aaagcagtag aattgccgat
     9601 acccaaaaat gtctgcgtgt ttcaggaaag cacaacgacc ttgaagacgt tggttttgac
     9661 acctatcatc acacgatgtt tgaaatgttg ggcaactggt catttggcga ttatttcaaa
     9721 aaagaagcac ttgcttgggc ttgggaattc ttaaccgaag tgttaaaatt agacaaagac
     9781 cgtttgtacg tttcggtttt tgaaggaaac ccgtcagaga acgtgccgtt tgaccaagaa
     9841 gcgtttgata tttggaagca atatgttccc gaagatagaa ttattttagg aaacaaaaaa
     9901 gacaacttct gggagatggg cgaccaaggt ccgtgtggtc cgtgttccga aatccatatt
     9961 gatttgcgca cagatgccga gagagctgcc gtttctggaa gaagcttagt caatgctgac
    10021 catccacaag tggtagaaat ttggaataat gtattcatgg aattcaaccg taaagccgat
    10081 ggttcgttag aaaaattacc tgcacaacac gtagataccg gtatgggatt tgagcgtttg
    10141 tgtatggcaa tgcaaaatgt aacatctaac tatgataccg atgttttcac gccattaatt
    10201 gcaaaaattg aacaaattac aagattaaaa tatacttcaa acgaagttaa aaacatatct
    10261 gaagagcaaa ataaaataaa tatcgctatc cgtgtgattg ttgatcatgc gcgcgcggta
    10321 gcttttgcta ttgccgatgg tcaattaccg tcaaatacag gtgctggtta tgttatccgt
    10381 aggatattgc gtcgtgcgat tcgctatggc tttacttttt tggataccaa agagcctttt
    10441 atctataaat tggtagaagt attggcacat caaatgggcg aattctttcc agagattaaa
    10501 tcgcaacaaa atttagtaac caatgtgatt cgagaagaag aagcttcgtt cttacgaacg
    10561 ttagaacaag gactacaatt gttagacaag gtggtagcag aaaccagtgg aaaagaagtt
    10621 tctggtgcca aagtttttga attgtacgac acgtttggtt tcccaaaaga cctaacggcg
    10681 ttgattttaa aggaaaaagg atattcgtat aacgaagaag attttgaagc cgaattacag
    10741 aaacaaaaag cacgttcgcg cgctgcttct gaggtttcta ccgaagattg gtcggtcttg
    10801 attcctggaa atgtagaatc atttgtgggg tatgaccaaa ccgaaaacga agtaaagatt
    10861 actcgtattc gtaaagtgga cagtaaaaaa gacggtgttt tataccaaat cgttttagat
    10921 gctacgccat tttatccaga aggcggcggg caagtaggcg ataaaggaac gttagtttct
    10981 gccaatgaaa ccattgaaat tatagatacc aagaaagaaa ataacttgat tctacacttc
    11041 gccaagcaac ttccagagaa tgtagaagct agttttgtgg ctaaagtaaa taccgctcta
    11101 agaacttcta cttcaaaaaa tcactctgct acgcacttga tgcacttggc tttgcgcacc
    11161 attttaggaa cgcacgtaga gcaaaaaggc tcgttggtga atcctaacta cctgcgtttt
    11221 gacttttctc attttagcaa agtaaccgac gaggaattaa aacaagtaga agattttgta
    11281 aaccaacgca ttgcggaaca actacaatta aacgaataca gaaacctacc tatacaggaa
    11341 gccttggcaa aaggtgctat ggcattgttt ggagaaaaat atggcgacag tgttcgcatg
    11401 attgaatttg gcgaaagcaa agaattgtgt ggtggaattc atgtaaaaaa cacggctgat
    11461 atttggcatt tcaaaatcat ttctgaaggt gctgttgcgg cgggaatccg tcgtatcgaa
    11521 gccataacgg gcgatgcagt aaaagagttc tacaccaatc aagaaaatac gttggcagaa
    11581 ttaaaagaaa ctttaaagaa tccgcaagat gttttaaaag cagtaaactc gttacaagac
    11641 gataacgcaa agctgaaaaa acaggttgag cagttgatta aagacaaagc gaaaggatta
    11701 aaaggcgaat tagcttctca aattcaagaa gtaaacggtg ttaaattctt ggctacacaa
    11761 gtagatttag atgccaatgg agctaaagat ttggcctacg atttgggcgc aaacgagacc
    11821 aatctgttta tagtttttgc aactgttacc gaagataaac cgatgttaac ctgctatatt
    11881 tctaaagaac tagtaagtga caaaggacta aacgccggac aagtagtgcg cgaacttgga
    11941 aaatacatac aaggcggcgg tggaggacag ccgttttttg ctactgctgg tggtaaaaat
    12001 gccgcaggaa ttccagaagc acttgaaaaa gcggttgatt ttgtgaacta atttcatgct
    12061 taaatatttt aatttaccca ttgcatagca ctgctataca atgggttttt ttgttgggtt
    12121 ttaggtgata attcaataat aatagaagtc cttaaaagaa ggtgttttca aagactcatc
    12181 tctgataaag ctttgtttta cggggattca ctgtgtctgt aaaatttcag agatttcatt
    12241 tcaaaaagac acttagattc cttgttttaa acggatttag tccatattaa taggaaaaac
    12301 aggtaactaa tcgagttttt ttgtttctta tcgttttttt tatgcgggtt acttttatag
    12361 ttttatgttt gacgtcccaa cccaaatcat aaaatctaac taacacctaa cctagttatg
    12421 aaacctactt ttaaacaaat catctttttg gtattctttt ttgttggaat tcaatttagc
    12481 ataagtgcta cttcaaaccc tacgactcct caaaatgaaa ccatactgtt aaaaaaactg
    12541 caatccaaag ttttgggtaa tgatagcgaa tatttgtttt cgtacaatga caaaaaaata
    12601 actaaaattg actatactac ttttgatggg gttactagaa aaggttattt taagtttacc
    12661 tattcaggag atttaatcac aaaaattcaa gaatttacaa cagagaacgt aaatcttttt
    12721 acttcaacat ttacttatac caatggtcaa ttggctggcg tggttacaga agaaatagga
    12781 aaaaacaagg cagaaaagat aaacttcata cacaatggaa ctgcttcagt aaccgcagta
    12841 agaagcattg gtacttctag tcaacaaaat aatatagttg aaaatgaagt gatgtatttt
    12901 acagacaata agttggttaa aaaggttgag aacggagtgg tttcaagttc aactatatat
    12961 gaatacgaca gagcaaacaa tccgttgcaa aatgtaattg gaatggataa gattaatgta
    13021 tatttaaaca cttcttttgg aatattttca tcaagcggat tagaaggaat tcaaaacaat
    13081 agagtaaaac aaacagtata tacttcaaaa ggaactttag aatatgtgtt ggtttttaac
    13141 accacatata ctgacgttaa tttcccaaac acaagaatgt ctccaccaaa ttctccagga
    13201 gcatttgaat acaactataa gtactagtgt ttttttgatt aaagaacctg cttttcgttc
    13261 tccaatttgt tgacgccaca tcaattttaa taataggaat actattttca aattaggcac
    13321 gaatatttct agattattaa tagatagcac tttaaaaaga atagtttttt ttagtgcatt
    13381 aaaataaata aactttggtt tgtttttttt tgtaatttgc attggattta aattttagga
    13441 tatgcgaaat tttttaatct tactgacaat ttgttcattt aattttttta gcggacaaaa
    13501 taatagattg cctgatggtg gagggtttta tgattttaat catcagaata ctgagtgctt
    13561 aagcgaagaa cagcgtctaa caattaaagg tcttttggat gaaaatatta agcatttgac
    13621 tcttgaaagt aaaattaacc tcaataaatc ttctgttttt aatccaacaa atcatccatt
    13681 gtttatatgg ccagttgtca aaagtgttac cgcaccctat aataatgtat ggtcgataag
    13741 taattatgtt gaccacaacc ccaattatcc taatgcagtt caagattgga attgcggaag
    13801 tagaacttat gatacagccg caggatataa ccacaaagga atagatattt ttacttggcc
    13861 tttctcttta tttcaaatgg ataatgatca agccgaggct gtagctgctg cagacggcat
    13921 tataattctt aaatctgatg gaaattttga tagaagttgt gcgttcaata acaataattg
    13981 gaatgcagtt tatatacagc atgctgatgg tagtcaatca tggtatggtc atcttaaaaa
    14041 cggatctcta acaaccaaag tagtaggaga ttttattgca gcaggtgaat atgtgggtat
    14101 agttggaagc tctggaaatt caacaggtcc acatcttcat tttgaagtgt ataataattc
    14161 aggtcagctt gttgatactt atctcggacc atgtaataat tggtcaagtt caacagaaac
    14221 ctggtgggaa aatcaaaagc catacctaga tccaaaaatt aatgctgttt tgtcacattc
    14281 tgcaccacca cagtttaata cctgtccaac aactgaaaca ccaaatataa gttcaagctt
    14341 ttctgctggt tcaaatgttg ttttagcgat ttatttagct gatcagttgg ctaattcaag
    14401 tgttaatttg gtgctaaaaa gacccgataa ttcaattcaa gccaattgga atttttcttt
    14461 agtaaacgaa tatagtgctt catggtggta ttggatattt ccatcaaatc aaatgattca
    14521 acaaggaaac tatcaattta cagcaacata ccaaggcagt tcagtaacac atgattttaa
    14581 ttatggaaac ttgggaattg atgaaagttt agcgtcaaaa ttttccataa ctccaaatcc
    14641 agttcaggaa agtttttcaa taaacggcag tgatactata attattgaaa gtgtaacaat
    14701 ttatgatata gtgggtaaaa aagtaattca gacaaattca tcatccaaag acataaatat
    14761 tgcagcactt gataatggtg tttatattgt taatgttatg acacttgaag gaagttatac
    14821 cgcaaaaatt atcaaaaagc aataactatt aatttttatc gttctccaat ttgttgacgc
    14881 cacatggcat aatacaatcc tttttcggca actaaatcgt catgtttccc ttcttcgatt
    14941 atttttcctt gttccaatac gaatatctta tcggcatgca ttactgttga aagacggtga
    15001 gcaattaaga ttgtaatcct gttttggtcg gatacggttc taatagtttc gttaatttct
    15061 tcttctgtaa tcgaatctaa agccgaagtt gcttcgtcaa aaatcaataa attcggattt
    15121 cttaaaatag cacgagcaat agataaacgt tgtttttcac caccagaaac ttttattcca
    15181 ccttcgccta tagtcgaatt caacccgtct tcggcacggt ctaatagttt ttgacaactt
    15241 gctttttgca tcacttcata caactcttca tcggaggcat ttggttttac aaacaataaa
    15301 ttctctctaa tagttcccga aaatagctga gcatcttggg ttacaaatcc taattgcttt
    15361 cgcaaatcca acaagtctat ctctttagaa tcaatgtcgt tgtacaatac ttttccttcg
    15421 gctggcggat ataaacctac taaaagtttt actaacgttg tttttcctga acccgatgga
    15481 ccaacaaaag caacagtttc accttgtttg atgtcaaaat taatgttttc aaccgctttt
    15541 tgagttgcgg ttttatgttt aaaagaaaca ttagaaaact ttaaagtggt aatcttttta
    15601 atgttttttg ggtttgcagg tctaaactct ttgggtgcgc taagcaaagt tttaaagttc
    15661 tccatagata ctttggtttc gttcaaagta ataataaagt tccccaattc ttgtaatgga
    15721 ccaaaaataa aaaaggtaaa aaacaccatc gttaataaat cacctacaat gatttttccg
    15781 ccaaataaga aataatacaa ggtaaaaaca acacatgttc ttaaaaaatg tacggttgta
    15841 ccttgaataa aactcaaact tcttataaaa cgaacctttt gtatttctaa ttgaagtatt
    15901 cttaatgtgt tgaggtttag tcttttttcc tcttgatgag ttaatcctaa actttttact
    15961 aattcaatat ttcgcaaact ctctgtagtt gaaccagcaa gcgaattagt ttgattaaca
    16021 atttttctag agactacttt tatcttcttg cctaaatagg agctaactaa tgcgataatt
    16081 ggtgcagtaa caagaaatat taatgatatt ctggcgtcaa ttcgagatac atataaaata
    16141 acaaaaacaa acccaatgat agtttggaat attaaggaaa tagaaagtgt aattagtttt
    16201 tcggagtctg ttcgtacttt ggataatttg ctcaaagttt cgccactacg ttggtcttca
    16261 aaatcttcaa aaggtaaatc aagcgatttt ttgatgccgt ctgtgtacat ttcggcaccc
    16321 gttcgttgga taactatatt ggtgaagtaa tcttgaaagt ttttagtaat tctagaaatc
    16381 attgcggcac caagcgacaa tcctaaccaa aaaccaagcg attttaaaaa accaactttg
    16441 tttccagcat aatttgcaat gttcacaccg caatcctgca atagtttacc cgtaattatt
    16501 gagtcagata aactaaaaca aatgttgatg gtagccatca ataaagcgaa aaaaagtaat
    16561 aatttatgtt tgagtatata agaataaagc aatttcatgt gtagcaaatt ttgaaatgca
    16621 aaagtactaa taatactcaa tcgagtagtt gttgtagatt attaaattgt tttgaagtta
    16681 aatttggtat ttttgaattc acaattcgtt attatggact ttagaacaat cattccaata
    16741 aaaaaatccc aaaacccaat tgattacaat agtcaattgc tgttaatggg ttcgtgtttt
    16801 gctgaaaaca tgggtgaaaa attggagtat tttaagtttc aaactacagt aaatccattt
    16861 ggaatcattt ttaatccagt ttcaatagaa aagttggttg accgcatggt aaatcaaaaa
    16921 ccatttacag accaagatgt atttttgcat aacgacttgt ggcattgtta tgaagtgcat
    16981 tcagggttga gtaatccaga taaagaagat tttttaaaat cattaaatca caccatcgac
    17041 caatcacatc aacaaattac caaagtaact catttccaaa ttacctacgg aacttcatgg
    17101 gtttaccgtc ataaaacatc gaatgaaatt gttgctaatt gtcataaagt gccgcaaaaa
    17161 gagtttgaaa aagagttgct ttcggtggaa caaattcaga gttctattca aaatacgatt
    17221 gctttagttc aaaaaatcaa tcccgattgc cattttattt tcaccatttc accagttcga
    17281 cacatcaaag acggttttat agaaaaccaa caaagtaaag cacatttagt aacagcactt
    17341 cagtcttcca tcttccatct tccaacttcc agttattttc caagttacga aatcatgatg
    17401 gacgaactgc gagattaccg tttttatgcc gaagatatgt tgcatcccag ccaagtggct
    17461 atagattata tctgggttcg cttttttgaa aattatgtat cagaaacaga gtttgagata
    17521 atgaatgaaa tttgtactat acaaaaatcg ctttctcaca aacctttcaa tcccgaaagt
    17581 gaaagccatc aaaagttttt agtttctctt gaacaaaaaa ttttaaagat aaaagaaaag
    17641 cttccacagg tgcagtttta aaaatagggc atctgcctta tttctttgag tttatatttt
    17701 tcagaggttt gtaaaataca attttataaa tctacacaaa tgaaaatatt tttaaaaaca
    17761 accaaagtta tctgcttttt taccatcatg gtttttaata gcagttttgc acaaacaact
    17821 ccaaccaata cctatactat tgatggcaaa atcaatttta tgaaactcac agataccgga
    17881 attctgctta ttgcacacaa tgatggattt gcaggtatta aaccagagtc aaataaatta
    17941 atctttgatt ttaaggatta tggctctgta aaagaagaag aattacagtt tattccagct
    18001 actccttatg ttgtaatttc tcaaggagga tttgcaaaat tatcgtctaa aaaaattgtc
    18061 tttgattatg tagtaggaaa acaacttttt gaaacgaaag aaaatggatg gaaagatgct
    18121 ttttctgcac aagtctttat tcctcaaaac aaattgatag tttcagggat tcgaacgtct
    18181 aaagaaaaat ttgcaatggc ggttggcatt tatgatttag taaccggaaa agaagaaaaa
    18241 ctaatttatc ctgttgaacc cggtaaagtt accatgggat ctgtatcggt tactggtagt
    18301 attttcatga aagacaattt ggtttacatt ccaacatcta aagagctaat ttgttatgat
    18361 attaaatcaa atagcagagt gtggtcagca aaagtggatg atttatcatg gatgacagca
    18421 gatgaaagtg gaaaagaaat ctatggattt gaaaacacaa atagtggtga cactaaaatt
    18481 aataagataa gcagtactgg agaggtattg tggaaagatt ctcaaaaggt aaaaggagca
    18541 gttactcgat ttgaaatatt accgcaaggt atagcaattg tgagtgatgt agctgataaa
    18601 ggtggaagtg tcttttcagc tagagcagaa tctaagatta tgatgttaag cgcaaaagat
    18661 ggagaagatt tatgggacaa agcaccaaaa acaaaaggat atgtacagca tttttatgta
    18721 atggatgacg gtatattatt tggtattcag tcgggtggaa tcaataaaat ttcatttact
    18781 ggagaaacac tgtttaaaaa accattaaaa acaggtgaaa acattcatac catggcttta
    18841 actccaaaag gaatgattta tatcactgat tcagatgcga acattattga tttaaaaaca
    18901 ggagaatcta tttggaacaa accaattaaa tataaaaatg caagtgctgt tgcaagcacc
    18961 tatgatgcaa aaaacaaaag atatttaatc agtactggtg atgatgtagt ggctattgat
    19021 gaaaatagcg gagatgtaaa aacgttatcg gaattcaaat ttgaagaaaa agaagctcca
    19081 aatagttttt cagttcgtgc taacggattg ctattaactt ctgaccaaaa tataatgatg
    19141 ctcaattttg atgggacaaa acaatttcat gaatattata aatcaccagg tagatcaaca
    19201 tttggtaaaa taatgggtgg cgttatggct gttgcttcta ctgcagcatc tgtttcgatg
    19261 gctgcaagag caggagcaaa taaaaattcg ataggtcagt ataacgagta tggtagagaa
    19321 gcggatagag cttcaaaaat gtttgccgac attggttctg cttcatttga ttatatgagt
    19381 aagcgtttca aagcctcggc ggcaactgca aatgcacagt ttatattaac aaaacttgat
    19441 gatggcgttg gcttagtaaa aatcaacaaa gattcaggtg caaaagagaa agaaattgta
    19501 ttgaaagaca aaaaaccaga atatgaagta gatgagtatg ccggagtttt atattataaa
    19561 aaagacaaca ataccattct ttcttatgat ttaaagtaag taatttattc actagtgtgc
    19621 tataaacaat tttttttaaa ctaatttaaa cattaataat tatgaaaaca atcaaacaaa
    19681 tcggtctaat tttatttgct acaatagcag tatctctctc ttcttgtagc aaaagcgatg
    19741 gaggaagcgg tggagacgct ggctcaggaa ctatgaaagc aaaagttaac ggaacaagcg
    19801 taacaactct agaaataaca acttttgcaa caatatctag taatgtgtta atccttcaag
    19861 gtaatacggg tggcacatct agcaaagcat tttatattaa tgtaggaagt tataatggtg
    19921 tgggaaccta tgatattggc ggtggtacta caggttttgg tcaagcaact gctagttata
    19981 tggaaatttt agttgatcca tcaaatccaa cagcatttga atcaactact tggcaagcac
    20041 catatgaagg aggagcaaag gttggtgaag ttagtgtttc tgaagtaaca agcagtaaca
    20101 ttaaaggaac gttccattac atggctaaaa aaaatacggg tgatggtgct ttaattgaaa
    20161 ttacagaagg ttcttttaac attcctttag attaaattgt aaataaaaaa attaaaaaag
    20221 acctgtgttt ccaggtcttt ttttgtcaaa aaattaagca accatcatat tataatattc
    20281 aatattaaat gtaatttttg ttagataagt ttgggaaact taatctttat tagctatcgt
    20341 tatgacctat ttattactat tacttttctt atttctagta ggcttggtgt tttttttaag
    20401 ctttaaaatt agtatagata gaaaaaactt taaaagtaaa atttcagcac ttgaggattt
    20461 tattattcaa ttgagtattg agcaaaaaaa gcaaaacaat cagttgattt tgtcagatga
    20521 tttaaaacaa aaaatagcct caataaacca aacaattagc cagaacattt atgatttaaa
    20581 ttatgaattg attgaagaaa attacacaaa aagaactgat tgatttgtat gtcatttttt
    20641 gtatatttat atgcataaac ttttactatg aggtgttttt attttttatt tttcatcttt
    20701 ttgtttcaaa atacttttaa tgctcaaagc cttcatgaaa cccttggcaa agcatttact
    20761 tcgtccgatt cgtccgatta ttattttaaa attgccaaaa aaagtattaa aacaaaagcc
    20821 gacgaaggac aatactactt ttgtaaaaat gcacgatgca ctgattataa tcaatttgat
    20881 agtgcagtag tatatggtga aagagccatc aaattattac aacgaaaaga agatttaaac
    20941 tcacttttaa cggtttattt aaatttcaat aaactctaca acaatcaagg acaatatgaa
    21001 aaagcaattg cctattcgct aaaaggattg aaaattgccg aaaaagaaaa aaatgaagct
    21061 tggattatcg agtactttat taatttatct gttgattatc acgactacga aagctttgaa
    21121 aaaggaattg attatggtaa aaaagcattg ctaaagctta agaaaaaaaa acagacaaat
    21181 cccggaagtc tttggagagc ctataattca atagccatta attttgacga ttggaaccaa
    21241 cccgatagtg cattgcatta tcacaaaaaa gcattgagct tctttaaagg aagcgacaca
    21301 ctcaaaatcc caacaacgtt caataatatt ggaaacacgt tactaaaaca aaaaaagtat
    21361 aaagaagcaa aacgatggtt gacatcgggt ttaaaaattt ctgaaatcag cgataaaaaa
    21421 gaagacattt atttctttta tggcagagcc actctatata caaatttagc cattattgaa
    21481 tcagaattag gcaattatca acaggcagaa aattggtttt ccaaggcgca tcaatatgca
    21541 gtaaaaagca atagtgccga aaagttaagg gatatttatg ccgccagagc aaagttttat
    21601 accaaaacaa aaaactatca aaaagctgtt gccgatcacg aagaatacat caaaataaga
    21661 gattctattt ttgatgcaga acgagacaaa accttttcgg agctagaagt aaaataccaa
    21721 agcgagaaaa aagaaaaaga aattcttcag gccaaagcaa aattatttaa aagccaaacc
    21781 gaaataaaaa gcaagaatac ccaattctta atattatccc taattaccat tgggttaatc
    21841 gtaatctcat tactaattta ccgtcagcaa aaactaaaac gaaatcaaca acaacaagaa
    21901 tttgagctaa aatcggccat tgcaaaaata gaaacacaaa acaaactaca agaacaacgc
    21961 cttcaaatat caagagattt acacgataat attggttcgc aactaacctt catcatttca
    22021 tctgttgaca atgtaaaata tgctttcaac atcaataatg ataaacttga caacaaactt
    22081 tcaggcatta gcgattttgc aaaagcaacc atcatagaac ttcgcgacac catttgggca
    22141 atgaataaaa gcgaaataac catcgaagat ttagaatcac gcatccataa ttttgtagaa
    22201 aaagccaaaa ttgccaaaac agaaattgcc ttcaatttcc atgttgacga ccgtttaaaa
    22261 gaagtaaaat tcacttccgt acaaggaatg aatatctata gaaccataca agaagccatc
    22321 aacaactgta taaaatatgc cgaagccaaa agtatctcaa tagaaattaa ccaaacaaat
    22381 gataccatag aaatcatcat tcaagacgac ggaaaaggat ttaatcaaaa ccaagtttct
    22441 tttggaaacg gtatcgaaaa tatgaaaaaa agagtaaaag atatcaacgg cgaaataaag
    22501 tttaattcaa taattgataa aggaacaacc attatcatcc gattaaaaaa ataaaaatat
    22561 gaataaggtt tacttcacat tgctactgct ttttttaaaa ggaacttttg gatttactca
    22621 aaatgcgcta gaactcatca ctaaactaaa aactgagtta aaaaccaatc cagatgataa
    22681 aagaaaagca acaatctatt cagacatcgc atggtattat gtcaatgttt ctaccgattc
    22741 ggctttggtt tacagcaaaa aagcgatgaa tgaatctacc aaactaaatg attcggtact
    22801 cattgcgcaa gttaatagtg atttgggtgc catatatttc agaagaaacg acttcgaaaa
    22861 cgcaaaaaag tattatggtg ttgcactaaa cattagaaaa gccagaaaag acaatgtagg
    22921 cattgccaaa ataaacctgc acatggcaaa catctacaac aaagaaaaca aaaaagaaaa
    22981 agctctacaa tattacttag caggcataga ctatttcgag aaaacaaaca accccgaaat
    23041 agtagcaaat accaaaggca atgtagccgc acttttcttt gaattgaaga attatccaaa
    23101 agccaaaaag tatttaaacg atgccataaa gtatcaagaa gcaaagaaag acgacaacgg
    23161 attgagcaca tcctatctaa cacttggaaa tatttattta aaaacaaatg acacgcttaa
    23221 cgcactaaaa ctctatgaaa aaagtatcgc ttctgcaaaa aaatcagcaa acaatgtagc
    23281 attaagtagc gccatgaaca accttggcgt aataaaatca gaacaaaaaa aatccagcga
    23341 agcacaaaca ctttttgcaa actcaaaagc agttaaagac agtttgagtg taaaaaaaga
    23401 cgcttccatt tccgatctca gtattgccaa agaacacatc atgtaccaac gtttcgatca
    23461 agcaaataaa gtactatatc aattaaaaga acgctatcaa aacaacccaa gtgagcaaga
    23521 attattgcta tctacctatc aattcttcat ccacacctat gcttatttac atcaacccga
    23581 tagcgttaat tactacaata acctagcaac aaacattcaa agcaaaatag tagaaacagc
    23641 aattgcaaaa caaaccaatg aactcgaaac aaaatatcaa acagccaaaa aagaaaaaca
    23701 attactttta aaagaaatag aaatcaaaca aacccgcgac cgattgtttt tagtaatcgg
    23761 aatagcacta atcattgcac taattgggtg gctaatttac cgtcaacaaa aaattagaaa
    23821 caaacaacaa caacaagaat ttgagttaaa atcggcaatt gcaaaaattg aaacacaaaa
    23881 caagttgcaa gagcaacgcc ttcaaatatc aagagattta cacgataata taggctcaca
    23941 actaaccttc atcatatcat ctgttgacaa tgtaaaatat gctttcaata tcaataacga
    24001 caaactagac aacaaacttt ctggaataag caattttgct agagaaacca ttattgagct
    24061 tcgcgacacc atttgggcaa tgaacaaaag tcaaattaca ctagaagacc ttgaatctcg
    24121 catccataat tttgtagaaa aagcaaaaat tgtcaaaacc gaaattgcct ttacattcaa
    24181 agttgacgaa cgtttaatcg atatacaatt cagttcggtt gaaggaatga atatctatag
    24241 ggccatacaa gaagccataa ataatagcat aaaatatgct aatgccacag ccatttctat
    24301 tgacatgttt aaagaaaatg ataccattaa agtaactatt aatgataatg gtataggttt
    24361 ttcttcggat gaagtaaatt taggaaacgg aattgtaaat atgcaaaaaa gaataaaaga
    24421 catcggagga acatttgaaa tgaatgccca aaaaggcata ggaaccacta ttatttttaa
    24481 agtgccaatg ccataaaccg ataaaatttt ttatttataa agtaatgcta caatattaaa
    24541 agttgagtaa aatcaaatca gttgagtaaa acagacaaat gttcggcagt taattactat
    24601 ttaaaagtgg ctattttttt aaaatcaatt ctcacaaact caaaaaaaaa ctatagagca
    24661 aaaaataaat agggcatttg ccgtatttcg ttttattgta attaaaatca tctttgtaac
    24721 tcaatttgtg gttttctaaa atataaaaaa taaaaactgg ttgtttttat tgttgtcctt
    24781 ttcaaatcat gtataagtac ccaaacactt ttgatttgga aaggactttt ttgtaaatta
    24841 catccatatt aaatacaacg caaaatgtca acacgtatag ccatagtgga cgataatgtt
    24901 tttttacaaa aagcagtagc cgaaaaatta tctttttttg atgatttaac actaaaatat
    24961 acagcaatta atggcaatga tttacttgca aaattagaaa aagacaaaaa cctcgaccta
    25021 attctaatgg acatcgaaat gcctgaatgc aacggcatcg aagcaaccaa gatagtcaaa
    25081 gcaaaatacc cacaactaaa aataataatg ctcaccgttt tcgataacga tgaaaacatt
    25141 ttcaacgcaa tcaaagcagg tgccgatgga tatcttttaa aagaagtaaa cccaaaagag
    25201 ctacaccaag gcatcataga aacactaaat ggtggcgcag ccatgaatcc atccattgcc
    25261 ttaaaaacat taaaattact tagaaaccca gtagattttg aagccaaaga acaagaagat
    25321 atcaaattaa caacccgcga agtagaagtt ttggaacagt taagcaaagg attaaattat
    25381 aatcaaattg ccgaaaatct ttttctgtca ccatcaacag ttcgaaaaca tatcgaaaat
    25441 atttatagca aacttcaagt gcattcaaaa atagaagccg ttcaaaaagc aaaagataat
    25501 aatataattt aaaacttttc caatcaatag gatttcataa aaatagggca actgccttat
    25561 ttttttgcgt ttgggcaaaa cctaattttg aactataact ttaaaatgat aattatgaat
    25621 gctaaaaaat tattttcgaa atggtcttta ggacttttat tatttggatt tggattggtt
    25681 tccaacgcca ataaccttca aatttcagga acagcagttt ctggatcaaa catctccttc
    25741 aacatcagtt gggaaaatag ttggaataca aacatcgctc cagccaattg ggatgccgtt
    25801 tgggtatttg taaaatacca agattgtgca acacgccagt ggtatcatgc agccattgca
    25861 accacaggga acacggctgc ttcaccacta caagtagact tagtaccaga tgcaaaagga
    25921 gttttcttaa gaagaagcgc ttttggtgga ggagacattg caagtacagc aatcacttta
    25981 gcattaaaca ttccagcagg aacctataac tacaaagtat ttggaatcga aatggtaaac
    26041 atcccacaag gcgatttcca agttggtgat gcaacaagta cagggacata tagtagtgca
    26101 acaataacgg catcttctca atcaggtgga ttatctgcag caacacttgg tggagcatca
    26161 gtaaatattc cagcaacatt cccaatgggg tacaactctt tctattctat gaaatatgaa
    26221 gtgacacaag aacaatatgt agaattttta aataccctaa catacgatca acaaaaagaa
    26281 cgtacaggaa tagaccctat cgctgcacca ggaaccggtg cttttactac gggtagccaa
    26341 tatagaaatg gtgtaacatt aataacatct ggtaacaaca gcactttgcc agccgttttt
    26401 gcttgtgatg ctacagctgg agttgaaaac aatgtagacg atggacaaaa cattgccatg
    26461 aactggatga gttgggctga tttagcagca tatttagatt gggcttgttt acgaccaatg
    26521 actgaattag aatttgaaaa aatctgtaga ggaactgcac cacgtgtagc aggagaatat
    26581 ccatggggaa caactgattt taattactat tcaacaggct caatagttgg tggaacgggt
    26641 tttaaatcga atgaaaatat ttcaaccatt gttaacggta gaaatttatg gtcaaattct
    26701 ggtgttgttg caggtgcagg atatgggcca tgtcgtgtag gtatgtttgc cacttcggtt
    26761 acaggtagat cgtcgtcagg agctgcttat tatggagtta tggaaatggc aggtaatgtt
    26821 tgggaaagaa cagttacaac gggtaatgca acaggagctg ctttcacagg agctttaggc
    26881 gatggtactt taacggtttt aggtgatgca gatgtggcta cttggccatc ttctacaaca
    26941 gcattaggtt tagggttaag aggtaacgga tattataata ctagtgccaa tccatcaaga
    27001 acttcagata gaacaaatgc tacgacagct agtccaacaa gaagttatac ctatggtggt
    27061 agaggtgttc gttaatcgtt tatttgataa tgacatattt aaatatgttc aataaaataa
    27121 tttttaatta ataaaatcaa ataacgatga aaacaacata tataaaaaag gttgtattca
    27181 tactgtttct attgtcaagt agttttattt attcgcaata caatggtgga gattcagatg
    27241 gattttcttc tagcagttta aataacacca gttgttcata tcctacacaa ttcattgctt
    27301 acaatggagg agatagtagc ttgacaacaa taaatagttt aataaataca acatgtaatg
    27361 caccaaacca gttctttgcc tacaatggag gcaatggtga cggttttgca tttgaatcaa
    27421 taaatgcagt ttcatgtgcc aatccaaatc agttttacgc ctattttggt ggtaatggtg
    27481 atggatttgc taatcaagct attaatgcaa tttcctgtcc aaatccgagt cagttttatg
    27541 cttacttcgg tggaaacgct gatggttttt ctgtgggaag tacagcacca gcttgtccaa
    27601 cagcaccacc agtggcagat tttacagctt cggcaacaac aatttgtgta ggtcaatcag
    27661 taacgtttac ggatacgtca accaattcgc catctgcatg gacatggaca tttactggtg
    27721 gaacaccaaa cagctcaaca gttcaaaatc ctgtagtggt atacaattca ccaggaacct
    27781 atactgtgac acttttggca gctaacttca atggtaatga tactgaaaca aaaacagctt
    27841 atattacggt aaatgccatt ccaacagtaa caactactac accggcttca cgatgtgatt
    27901 cgggtacagt aacattacaa gctacggcaa gttcgggagc tactttaaat tggtatgcag
    27961 cagcaacagg cggaacggct ttgtcatcag gaacaagttt tacaactcca agtttaacag
    28021 ctacaaatac atattacgtt gaagcacaat ttggaacctg tatttctgct cgaacagccg
    28081 taattgcaac agtaaatgct acaccttcaa ttacagcaac aactcctgca tcaagatgtg
    28141 gaaccggaag cgtaacttta caagctactg caagcgcagg aactattcaa tggtttgcag
    28201 atgcagctgg tggaacggca ttatttacag gagcaaatta tacaacacca tctttaacta
    28261 caacaacaag ttactttgtt caagtaagtc aaaatggatg tatttctcca agaaccgaag
    28321 ttgtagcaac aattaatact acgccaacaa ttacaggaac aactcctgcc acacgttgcg
    28381 atgctggaac tttaacgtta caagcagtag caagtgccgg aacaataagt tggtatgcca
    28441 cagcaacagg cggaacagct ttggcaacag gaacgagttt tacaacacca agtttgtcaa
    28501 caacaactac ttactatgtt gggacggcaa cggctacttg tacatcaaca cgaactgctg
    28561 taatagccac agtaaatgca acgccttcaa taactggcac aacaccagga agtgtttgtg
    28621 atagcggtac ggtttcacta tctgcatcaa caagtgcagg aataataagt tggtatgctg
    28681 ttgcatctgg cggaactgtt ttaggaacag gtacaacttt tattactcca agtttgacag
    28741 caacaaccac ttattatgca gaagcatcaa atggtggttg tacttcagca aggacagcgg
    28801 taattgcaac ggtaaatact acgccaacaa ttacaggaac aactccagca agtcgttgtg
    28861 gaaacggaag tgttacgcta caagctacag caaatgttgg aacaatcaga tggtttggct
    28921 cagcaactgg cggaagtgtt ttagcaactg gatcatctta tgtaacacca aatttaaatt
    28981 ctagcacgac gtattatgtt gaggttacac aaaacaattg tacttcacca agaacagctg
    29041 tactagcaac aattaataca attccaacca tcacgggaac aactccagcc aatcgatgtg
    29101 gagatggttc cgttacgctt caagctacag caagttcagg aacaatttct tggtatgcca
    29161 ctgcaaccgg tggaacagct ttagctacag gtggaagttt ta
//
LOCUS       DAOOBH010000015        28637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_2147_length_28637_cov_7.768876, whole genome shotgun sequence.
ACCESSION   DAOOBH010000015 DAOOBH010000000
VERSION     DAOOBH010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 28637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28637
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2147_length_28637_cov_7.768876"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PK218_03250"
     CDS             <1..293
                     /locus_tag="PK218_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97556.1"
                     /translation="KAIELEPKFSEEDLKKQIESGKANFINFGITIADFSEFQKKYGI
                     GNSGQGCMITPDLSYTATQNNQYLAKYLDEKYGEIWRKDLPFLPYSLVDKEN"
     gene            480..737
                     /locus_tag="PK218_03255"
     CDS             480..737
                     /locus_tag="PK218_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97557.1"
                     /translation="MNNEDLKREYLENRSFDDSHFKKMILDYLRTYGRCKRDKIDNLI
                     IPKLSKALSEEKKRNKVTNYLSSLRMEGKIVNTPGYFWETV"
     gene            802..>1535
                     /gene="hemF"
                     /locus_tag="PK218_03260"
     CDS             802..>1535
                     /gene="hemF"
                     /locus_tag="PK218_03260"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HPW97558.1"
                     /translation="MKNKFYTYIQTLQDQICRRLEDIDVGTKFREDIWERPEGGGGKT
                     RVIENGNVFEKGGVNISAVHGKLPETMQKMFNVGEADFFACGLSLVIHPKSPMVPTVH
                     ANWRYFEMYDDNGEVINSWFGGGQDLTPYYMFEEDGKHFHQTCKNACDKHNLDFYPKY
                     KKQCDAYFWNAHRNEARGLGGLFFDYCKATDDMKMEDWYNFVTEVGNPFLEAYVPIVE
                     KRKNSAYTAEQRNWQEIRRGRYVEFNL"
     assembly_gap    1536..1635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1636..1859
                     /locus_tag="PK218_03265"
     CDS             <1636..1859
                     /locus_tag="PK218_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HPW97559.1"
                     /translation="EQRNWQEIRRGRYVEFNLVHDKGTLFGLKTNGRIESILMSLPPH
                     VQWVYDHHPEKGSEEEKLLKILEKPIDWV"
     gene            1923..2921
                     /gene="hemB"
                     /locus_tag="PK218_03270"
     CDS             1923..2921
                     /gene="hemB"
                     /locus_tag="PK218_03270"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPW97560.1"
                     /translation="MFPLQRGRRLRVNESIRSLVRETSLSPADFMFPMFIMEGENTKV
                     EIPSMPGIYRRTLDLTVEEVKELFTLGIRAVNIYVKVNDNLKDNTGKEAWNSDGLMQN
                     AIRAIKKACPEMIVMPDVALDPYSIYGHDGIIEKGDIANDKTNDALVKMAVSHAEAGA
                     DFVAPSDMMDGRVLRLRQGLDAAGFQNVGIMSYSAKYASAFYGPFRDALDSAPKEADV
                     VAPKDKKTYQMDYSNRIEAVKEALWDVEEGADMVMVKPGIAYLDIVREVKNAVNVPVT
                     VFHVSGEYAMVKAAAERGWLDHDKIMMEQLMCIKRAGASLISTYFAKEAAILLNKQ"
     gene            2921..3286
                     /locus_tag="PK218_03275"
     CDS             2921..3286
                     /locus_tag="PK218_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPW97561.1"
                     /translation="MKRVTFFALTVLLFSCKKESEDNFGQPKSEATEKISGKELFETK
                     GNCVACHMTNQKVIGPSLEEISKIYKDKNASIVSFLKEESQPIVDPSQYEVMKTNFAI
                     TKKMSDDELKALEDYIFAH"
     gene            3313..3783
                     /locus_tag="PK218_03280"
     CDS             3313..3783
                     /locus_tag="PK218_03280"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPW97562.1"
                     /translation="METCFINSPLGITKIVGDENGISVISILAEGEVSKTIPKPLKEC
                     VAQLGDYFDGKRTEFTFTLNPTGTDFQQRVWQELLKIPFGKTISYLDLSKKTGDAKAI
                     RAVASANGKNPLWIVIPCHRVIGTDGSLTGYAGGLWRKKWLLEHENPSNQQSLF"
     gene            3879..4592
                     /locus_tag="PK218_03285"
     CDS             3879..4592
                     /locus_tag="PK218_03285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPW97563.1"
                     /translation="MIEKINLNNILFLDIETVPIEENFNNLDDEMQQLWEQKTQYQRR
                     DEFTPDEFYDRAGIWAEFGKIVCISAGYFVNKSDIRNFRVTSFFGDEVKILKDFSNLL
                     NNHYNQPQHVLCGHNAKEFDIPFIARRMIINQVAIPDKLNLFGKKPWEIPHLDTLELW
                     KFGDYKHFTSLKLLTKILGIPSPKGDIDGSQVGHVFYVEKDIDRIVTYCEKDTIAVAQ
                     IFLRLRREDLLVDDEIIHV"
     gene            4641..6080
                     /locus_tag="PK218_03290"
     CDS             4641..6080
                     /locus_tag="PK218_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPW97564.1"
                     /translation="MVLSRFWLAIFISSIVFVIISLFSGTNYTIDYVLNGEKGNPILI
                     SEKYIDQVPAFIRDSIQLKEDKTMIVNRDLSNPDTTYVYKNKTVQIYSGVQKSDGLLA
                     TCKSSLVDIIIPLIAYLAFFCGLMELLIISGVSGKLAKVLSPVFVKVFPSIPKNHPSI
                     SYMTLNFAANFLGLDSAATPFGLKAMESLQEINPEKEKASDAQIMFMCLHASGLTLIA
                     TSIIGYRAAAGSSNPADVMLPCIITSFIGTIAAFIIVGIKQKINFKSASLAIGLMTLI
                     GAIIGLLMYVNHLDLIGKNYFTSNLSGLILLTIIVFTLIYAFRKEKLFAEAKTTVFDA
                     FVTGANNGVKTGVTIFPYVLGMLAAISLFRNSGLFDIISNSLAFVFSIVGLNKEIIDA
                     LPVALLRPFSSAGSRGFLIDSMNTFGADSLTGRLSSIFQCSAESTFYVIAVYFGSVNI
                     KNTRYALTTMLLVDLICVITAIFVATWFF"
     gene            6250..6801
                     /locus_tag="PK218_03295"
     CDS             6250..6801
                     /locus_tag="PK218_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97565.1"
                     /translation="MKLEVIEKDHFYHIYNRGINSELIFHSNENKSYFLTLFNKHLHQ
                     NVATYAYCLMDNHYHFVLQITEEEKKVTQAFSNFFNAYAKAFNIQNSRTGSLFEKHFK
                     RIKIEDENFLRNIIQYVHLNPKHHLDINYKTYKYSSFQTILSNKPTKLRRIEVLKLFG
                     DVDNFIYCHDYKNDLLSEKFTFE"
     gene            6882..8483
                     /locus_tag="PK218_03300"
     CDS             6882..8483
                     /locus_tag="PK218_03300"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPW97566.1"
                     /translation="MDFIYQEPFPILKDDTQYKKITSDYVKVEKIGNREILNVDPKGL
                     ELLSQVAMNDVSFMLRTSHLQKLKNILDDPEATDNDRFVAYNLLQNAVVAIEGELPSC
                     QDTGTAIVMAKKGEDVYTGVDDAEWLSKGIFNTYQEKNLRYSQIVPISMFEEKNSGSN
                     LPAQIDIYAKKGNYYEFLFLAKGGGSANKTFLYQKTKSLLNDKSLDEFVRERIKDLGT
                     SACPPYHLALVIGGTSAEANLAAVKKASAGYFDNLPTSGNMAGQAFRDLEWEQKVLKI
                     CQESAIGAQFGGKYFAHDVRVIRLPRHAASCPVGLGVSCSADRNIKGKITKDGIFVEQ
                     LEANPKQFLPEVAPHLEAPVEVDLNRPMKEILAELTKYPIKTRLKLNGTLIVARDIAH
                     AKIKELLDTGKQMPEYFKNHPIYYAGPAKTPEGMPSGSFGPTTAGRMDVYVEEFQKAG
                     GSMIMLAKGNRTKEVTNACNTYGGFYLGSIGGPAAILAKENILKVEVVDFPELGMEAV
                     RKIEVKDFPAFIITDDKGNDFFANL"
     gene            8486..8878
                     /locus_tag="PK218_03305"
     CDS             8486..8878
                     /locus_tag="PK218_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97567.1"
                     /translation="MDFKETFSEFYSKVKNSIADGTFAKLTLAKTIGNTDLMNIYVRP
                     IVDEDVLKLELKFKFQKEEIIEIRTIEETFDRLLEFINNPFLSAILFTTAFDLTYKLN
                     KKRAVSMIEQFPSFGNANEVLLEFLANK"
     gene            complement(8949..9608)
                     /locus_tag="PK218_03310"
     CDS             complement(8949..9608)
                     /locus_tag="PK218_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HPW97568.1"
                     /translation="MALDKNQIAKRIAKEVKDGYYVNLGIGIPTLVANYVRTDISVEF
                     QSENGVLGMGPFPYEGEEDADIINAGKQTITTLPGASFFDSAFSFGMIRSQKVDLTIL
                     GAMEVAENGDIANWKIPGKMVKGMGGAMDLVASAENIIVAMMHVNKAGESKILKRCTL
                     PLTGVGCVKKVVTELAVMEITPKGFKLVERAPDVTVEHIIASTEAELIIEGEIPEMII
                     D"
     gene            complement(9642..9995)
                     /locus_tag="PK218_03315"
     CDS             complement(9642..9995)
                     /locus_tag="PK218_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPW97569.1"
                     /translation="MRNDKDNLIVKLTFEFALEIINYSEEIRKTNRFEMASQIFRSGT
                     SIGANIREAQNAESKADFIHKFKISAKETDELQYWLDLCLNSEFYPNPSEELLKKLQS
                     INLIISKIISSSKKG"
     gene            complement(10027..>10347)
                     /locus_tag="PK218_03320"
     CDS             complement(10027..>10347)
                     /locus_tag="PK218_03320"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPW97570.1"
                     /translation="EVADGKETREFNGKMHVLEHAFKADFSIVKAWKGDEAGNLIFKG
                     TARNFNAPMAGAAKITIAEVEELLPVGSLDPNQIHIPGIMIQRIFQGETFEKRIEQRT
                     VRQK"
     assembly_gap    10348..10447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10448..10577)
                     /locus_tag="PK218_03325"
     CDS             complement(<10448..10577)
                     /locus_tag="PK218_03325"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPW97571.1"
                     /translation="MINKKVNSVTEALQGIEDNMTIMLGGFGLCGIPENSIAELVKK"
     gene            complement(10666..12969)
                     /locus_tag="PK218_03330"
     CDS             complement(10666..12969)
                     /locus_tag="PK218_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPW97572.1"
                     /translation="MAQNKKNTTNDIKYYKRKFWQFFIYGFSALILFFLLASWGFFGA
                     MPTFEELENPESNLATEVISSDGVTIGKFYNENRTPIKYEDLPPHLVNTLIATEDERF
                     YEHSGIDARRTFGAAIKLGADGGASTITQQLAKQLFHGEGSTFKIFRLVQKVKEWIIA
                     IKLERQYTKNEIIAMYFNVYDFNNYAIGIRSASKTYFNKEPKDLTIDESAILVGMFKN
                     SSLYNPVRNPQGVKNRRDVVLGQMVRSKIITEAQKESYSKLPIKLDFKLQTHKEGIAT
                     YFREFLREYMKNWVKENKKPDGTEYDIYTDGLKIYTTIDSKMQEHAEEAVQAHIKNLQ
                     EEFFIQQKGNKNAPFLKISVDETNRILKRAMKSSERWHILKDLDKTDDEIIASFSKKT
                     KMRVFTWKGEKDTIMTPLDSIRYYKSFLQAGMMSMEPQTGHIKAWVGGINYKYFQYDH
                     VGQGARQVGSTFKPFVYATAIEQLGMSPCDSIIDSPYTIPVGRHHVTAAWTPKNSDNK
                     YRGMVTLKKALANSINTVSAKLMDKVGPEAVVNLTKKLGVKSEIPLQPSIALGAVEVT
                     VEDMVAAYSTFANQGVYIKPQFIDRIEDKNGVVLYEPIPESHDVLNKDIAFAVIKLLE
                     GVTEGGSGSRLRTQGGGYGYERVTGYPYAFTNPIAGKTGTSQNQSDGWFIGMVPNLVS
                     GVWVGCEDRSARFKSITYGQGATAALPIWGYFMKKCYEDKDLQVSKDEFERPKNLSIK
                     VDCWAPKVVDSLELELQKEQDTDEFGL"
     gene            complement(12979..13464)
                     /locus_tag="PK218_03335"
     CDS             complement(12979..13464)
                     /locus_tag="PK218_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HPW97573.1"
                     /translation="MMLKNSLVLLFICMCLVSCDKNRVFDEYKSVGNSWHKDSVVTFE
                     LPELQKDKTYNLFLNVRDNDDYPFNNLFLIVSLEQPNKKIKVDTLEYVMTHPDGTLLG
                     EGFSDIKENKLFYKANEKFDQKGVYKIHIIQAVRQTGKIEGVEQLKGITDVGFRIESK
                     E"
     gene            complement(13448..14656)
                     /gene="ricT"
                     /locus_tag="PK218_03340"
     CDS             complement(13448..14656)
                     /gene="ricT"
                     /locus_tag="PK218_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPW97574.1"
                     /translation="MACTNCSTSTKDGTPRGCKNNGTCGTDSCNKLTVFDWLSNMSLP
                     NGEVAFDCVEVRFKNGRKEFYRNTEKITLSIGDVVATEASPGHDVGIVTLTGELVKIQ
                     MKKKGVDFKGEVPKIYRKASQKDIDIWSDARDKEEPMKVKAREIAIRLNLEMKISDIE
                     FQGDGSKAIFYYTANDRVDFRQLIKEFAQSFKTRIEMKQVGFRQEAARLGGIGSCGRE
                     LCCSTWLTDFRSVNTSAARYQQLSLNPQKLAGQCGKLKCCLNYELDTYLDALKDFPEF
                     ETKLHTEKGDAICQKQDIFKGLMWFAYTDNFANWHVLKIEHVKEIIALNKEKKRATSL
                     EDFALEMAPLPEKDFNNAMGQESLTRFDQPKRKNKNNKKKKPSNQNNSNNTNNSKKKP
                     IIISKNDVKK"
     gene            complement(14783..15817)
                     /locus_tag="PK218_03345"
     CDS             complement(14783..15817)
                     /locus_tag="PK218_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW97575.1"
                     /translation="MDIYKLLKFNHAIKSHRIKFFGLWLLSVLNKRYLAIQFDPVLAC
                     NLRCKMCYFTDDEYVRTNMKGIFKENDLEELAKVNFKNALKLQIGCGAEPTLFKHNVQ
                     LIKIAKKHKVPYISMVTNGNLLENQDIIDLSQAGLNEFIFSMHGVFKETYEDLMDKGV
                     FEKFHAVLGYITEEKKKNPTLRLRINYTFNVDNFHELKEFFKVYDKYSIDIIQLRPID
                     KIGETTYNNFSLKAIENDYQEINTFLKKEASKRGITLLSPKTISRNEEQSLKVKTEND
                     SSYLIPYTYCYVSPKYYWKDDFDWKTETFSEWQKRNSWNKILFKNIFLSKKNLEKPNR
                     NMLNYSVELN"
     gene            complement(15827..17185)
                     /locus_tag="PK218_03350"
     CDS             complement(15827..17185)
                     /locus_tag="PK218_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HPW97576.1"
                     /translation="MQLYNTLSAEERKELIDLAGKQRLTLSFYAYAKIEDPKKFRDDL
                     FLAWNALDALGRIYVAHEGINAQMSVPADNFEEFRETLEVYDFMKGIRLNVAVEQDDH
                     SFLKLTIKVRHKIVADGLNDETFDVTKKGIHLGAKEFNEILEDPNTIVVDFRNHYESE
                     VGHFKDAITPDVETFRESLPIINEQLKDYKETKNLVMYCTGGIRCEKASAYYKHQGFK
                     NVYQLEGGIINYAKQIKEEGLESKFIGKNFVFDNRLGERITDDIVAQCHQCGKPCDNH
                     TNCANDGCHLLFIQCDECQAVMENCCSTECLDITHLSLEEQVRLRSGKQVGNKVFRKG
                     KSEKLKFKHSGELSDVALAVAKKTKDIRQKIKAKKVILGKAEHYFVKAGIGLFIIENN
                     ELQLGDKILISGPTTGNQELILEKMFVNGCENSAAKIGDKVTFEVPFRVRLSDKLFKL
                     VN"
     gene            complement(17379..17789)
                     /locus_tag="PK218_03355"
     CDS             complement(17379..17789)
                     /locus_tag="PK218_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HPW97577.1"
                     /translation="MYPEEMVKPMRAELSEAGFQDLYSAEAVKNAIKEEGTTLVVVNS
                     VCGCAARNARPGAKMSLDGSKKPDHLVTVFAGVDKEAVDAAREEMFPFPPSSPSMALF
                     KNGELVHMLERHHIEGRPAEMIAENLKDAYAEFC"
     gene            complement(17915..19078)
                     /locus_tag="PK218_03360"
     CDS             complement(17915..19078)
                     /locus_tag="PK218_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HPW97578.1"
                     /translation="MQHYHYIFAGSGLSALMTVCEMIRSEDFQGKSILLLDIDSKKNN
                     DRTWCFWDDTNLFDEITSKKWNEALFKNATYKRVLNLNPYQYTMVRGLNFYNMVFELI
                     SKQDNIHFSNQKVVYFEELGNHCVLKTETHNYTCNKIFNSIFNPKPIKNQSKYPLLQQ
                     HFIGWFIKSKEAVFNPNCATFMDFSVEQKGNTRFMYVLPTSETEALLEYTLFSKDLLS
                     KNEYEKEIKNYIEKLGVTEFEIVEKEQGNIPMTCYKFWKNNTKNIVNIGSAGGWTKAS
                     TGFTFKNVTKKSKELVAFLETKNSNSHNETDFRKLHKKTKYWLYDLLFIDVLFQNNAL
                     GSLVFSSLFKKGNPTLIFRFLDEETTFLEDLQVIWKCPKIPFIKALFGRIFTF"
     gene            19196..19714
                     /locus_tag="PK218_03365"
     CDS             19196..19714
                     /locus_tag="PK218_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPW97579.1"
                     /translation="MLSKNIEVALNKQIRIEAESSQTYLSMACWAEVNGLEGISNFMY
                     AQSDEERQHMLKLIKYVNERGGHAKISELSAPKTEFDNFKNMFELLYKHELFVSESIN
                     ELVRITFEEKDYSTHNFLQWYVSEQIEEESQAKTILDKINLIGDDKGGLYLFDRDIQS
                     IAAANSASNVKN"
     gene            19781..19963
                     /locus_tag="PK218_03370"
     CDS             19781..19963
                     /locus_tag="PK218_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97580.1"
                     /translation="MASKVKDKKKKKDKKKDSIDVLILLKNLDACKSSCCEKYKKNEK
                     KRCKRCPMFDLLKSIN"
     gene            complement(19991..20740)
                     /locus_tag="PK218_03375"
     CDS             complement(19991..20740)
                     /locus_tag="PK218_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPW97581.1"
                     /translation="MKSKILFFALFLYSTIFAQEKEIEPIQTDRPDQTETPSLTPKGM
                     LQVETGFSFQKNDVNSKTNLLPSTLWKYGFNENFELRLITEFASEKFNVETITGFNPI
                     LVGCKIKIAEENGVFPKTSFIGHVSLPNVASTAFITDYFAPEFLFVMQHTLSEKFSLS
                     YNLGAEWDGFTPEPTFIYTVATGYSITEKLGSYIELFGFAPQNNEANHNFDGGITYLI
                     STNFMLDLSSGFGISENAPDNYFALGFSFRL"
     gene            20819..21478
                     /locus_tag="PK218_03380"
     CDS             20819..21478
                     /locus_tag="PK218_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPW97582.1"
                     /translation="MTNSEENYLKVIYHLSLVSPKGVNTNAIAGMLETKASSVTDMMK
                     KLAEKELVLYQKYQGVTLTEKGFLSAKMIVRKHRLWEVFLVEKLDFAWDEVHEIAEEL
                     EHIKSENLINKLDEFLGFPAFDPHGDPIPNENGEIQKVSKLLLSESEINREYQCVGVK
                     DSSSDFLQYLDKQNIALGSKIKVINKEQFDETLTIEVNNTSLTISNKIAKNLFVKNKS
                     L"
     gene            21475..23346
                     /locus_tag="PK218_03385"
     CDS             21475..23346
                     /locus_tag="PK218_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPW97583.1"
                     /translation="MSKSLEEVNESIATQGKKSTFRKILAFFGPAYLISVGYMDPGNW
                     ATDLAGGSQFGYALIWVLLMSNIMALLLQSLSARLGIVTQRDLAQASRETYSPFINYI
                     LYFLAEIAIAACDLAEVLGMAIGLNLLFDISLINGVLITVLDTFLLLFLINKGMRKME
                     AFIIALVAIIGFSFVFEMIFAEPEVSKIFAGLVPSLPGEAALYIAIGIIGATVMPHNL
                     YLHSSLVQTRKFDRSDTGIKQALKYNFLDSAIALNLAFFVNAAILILAAATFYKNGMF
                     EVAEIQDAHMLLEPLLGTHWAPILFAVALIAAGQSSTITGTLAGQIIMEGYLNLRIQP
                     WVRRIITRLIAIVPAVVTILYFGESVTGKLLILSQVILSLQLGFAIIPLIHFVSDKTK
                     MKGFHIGKATQIASWLIALIIVSLNGKLVYDEIKGWLETAENPTVIWFTVVPLTIGFL
                     FLLLFIIFKPFITKTKNNIENHSPHSLKLNFEETKINDKKDIAVSVDFSTADEIALNA
                     AIELGGRNANYTLIHIVETVGAMIYGEKTDDHETTIDEKLLLEYQEMLKEKGFNVSTK
                     LGFGKPFKAIPTIINEGKYDVLVMGTHGHTGIKDLLFGTTVNKLRHKISIPLLIVKN"
     gene            complement(23343..23468)
                     /locus_tag="PK218_03390"
     CDS             complement(23343..23468)
                     /locus_tag="PK218_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HPW97584.1"
                     /translation="MNVQEILVYIALGIAIGFLVTKFIWKPKSKKGCDKDDCGCH"
     gene            complement(23474..25582)
                     /gene="feoB"
                     /locus_tag="PK218_03395"
     CDS             complement(23474..25582)
                     /gene="feoB"
                     /locus_tag="PK218_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPW97585.1"
                     /translation="MKSHQNINVALIGNPNTGKTSVFNQLTGLNQQVGNYPGITVDKK
                     LGFCKLPNNIKANILDLPGTYSLNASSIDENVVIELLLNKNDKLFPDVCVVVTDVENL
                     KRNLLLFTQIKDLEIPTILVINMADRMKIKGISLDIPLLEEQLKTKIVLVSTRRGIGI
                     SELKNAIVDYKNLSTEPCLNASTIDAEYFDKLRNAFPNQLVYKLWLVITQDVNFLNLD
                     RNEIRSSFTKSHSDLKRLQQKETIKRYQFINDTLKIGQKIDVSKATDIRAKLDRILTH
                     KIFGYLIFFGILLLIFQSIFDWSSVPMDWIDSTFANFSTYAKNHLAPGEFTNLISEGI
                     IPGIGGIVIFIPQIAFLFLFISILEESGYMSRVVFLMDKIMRRYGLSGKSVVPLISGT
                     ACAIPAIMGARNIENWKERLITILVTPFITCSARLPVYAILIALIIPDKRILGLFSLQ
                     GATLMILYLLGFGMAIFSAFILNKILKLKTKAYFVVEMPSYKIPMFKNIAINVIEKTK
                     AFVFGAGKIILAISIVLWFLGSHGPNTEFKNAQKIIEKEVYNSDLKINPSVIPDRVAE
                     YKLENSYIGIIGKSMEPVIKPLGYDWKIGIAVVSSFAAREVFVGTLATIYSVGSHSDE
                     ETTIKKRMEAEINPDGSKIFNFATGISLLLFYAFAMQCVSTLAIVKKETNSWKWPIIQ
                     LVFMSGFAYITALIAYQLLK"
     gene            complement(25586..25822)
                     /locus_tag="PK218_03400"
     CDS             complement(25586..25822)
                     /locus_tag="PK218_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPW97586.1"
                     /translation="MRTTVNLLKRGQKATIIQIDVDKIPLKLLEMGCLPGNELEVVQI
                     APFGDPIYLNVNNGSHLAIRLETASEIEVELLNF"
     gene            complement(25906..26574)
                     /locus_tag="PK218_03405"
     CDS             complement(25906..26574)
                     /locus_tag="PK218_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HPW97587.1"
                     /translation="MLSFFKKYRWFFIGVLVFSAIVVSLFYSALKPKKTLPIYNPADV
                     NPELVDSTVQYVEKYHTIADFSFTNQNGKTVTQKDYEGKIYVADFFFTTCQSICPKMT
                     ANMVDVQKAILNNPKVMLLSHTVTPEIDSVSVLKKYAIEKGVIDSKWNLVTGDKKDIY
                     FIARKSYLAVKTGKPEELYDMVHTENFVLVDRKRRVRGFYDGTNPDEIKKLIDDIKWL
                     SDNE"
     gene            26731..>28637
                     /locus_tag="PK218_03410"
     CDS             26731..>28637
                     /locus_tag="PK218_03410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HPW97588.1"
                     /translation="MIVILITAFGFNQNNAQQNGVGIDLSLMDTSVSPKDDFFNFVNG
                     TWLKNTEIPADRTRWGSFDELRKKTDADALAILAEASKNPKYKSDTDQGKAINLYKSI
                     MDTVARNKAGIKPLLPYLTKINAVKNVSDLQNLMIQMASKGGVGFFGIGVGTDAKNSS
                     KNVVYVGPGSLGLPDRDYYVSDDADSKEKREKYVLHIARMLQYIGEKPQEAKIHAEKI
                     LALETEMSRPRLDRVERRDRRKSYNPMTIADLQKMTNAINWEKQITGIGLPKVDTLIV
                     SQPKYMAALQDILKANKVEDWKAYMRWTALNSAADNLSTDIEKANWDFYSKTLNGAIK
                     QRPRDEGALQVVNGATGEALGKLYVEKMFPAAAKEKAQKMIKNVIRAYETRINNLSWM
                     SPDTKKSAVEKLNKMTIKIGYPDKWKDYSALTIKSPAEGGTYFENSLLISEWSFKKNI
                     EKLSKPVDKTEWGMSPQTVNAYFNPSNNEIVFPAAILQPPFYDYNADEAVNYGGIGAV
                     IGHEISHGFDDSGARYNADGNLVNWWTDEDLKQFTALGTALADQYSALEPLPGIKVDG
                     KFTLGENIGDLGGINAAYDGLQLYLKENGNPGKIDGFTPEQRLFISWATIWRSKMRDE
                     AIKNQVKTDPHS"
BASE COUNT         9596 a         4501 c         4670 g         9670 t
ORIGIN      
        1 caaaagcaat tgaactagaa ccaaaattct ccgaagaaga tttaaaaaag caaatagaat
       61 caggtaaagc caatttcatc aattttggaa ttacaatcgc agatttttca gaatttcaaa
      121 aaaaatatgg cattggaaat tctgggcaag gttgcatgat tactccagat ctttcttaca
      181 cagcaacaca aaataatcaa tatctcgcaa aatatttaga tgaaaaatat ggggaaattt
      241 ggagaaaaga cctgccgttt ttgccgtata gtttggtaga taaagaaaat tagaaaaata
      301 atttttttat ttaccaaaag gttattatat ttgttttaaa ttcatagagg aaatcatgag
      361 tattcaatta aggaacgaaa aaagaaatta ctgaaagtga gttcaaacac cttaaaaagt
      421 gcaaatatat tgaaggcaga aaaccaaaca tttatctttc tcatgatatt gttgtttctg
      481 tgaataatga agatttaaaa agagaatatc ttgaaaatag aagttttgat gattctcatt
      541 ttaaaaaaat gattttagat tatcttagaa cttatggaag atgtaaaaga gataaaattg
      601 ataatttaat tattcctaaa ctatctaaag cattatccga agaaaagaag agaaacaagg
      661 tgactaatta cttatcttcc ttaagaatgg aaggaaaaat tgttaatacc ccaggttatt
      721 tttgggaaac cgtttaaata ctttttaaat acttttaaac actttttaaa tacttttaaa
      781 cactttttaa acacttttaa attgaaaaat aaattctaca cctacataca aaccctacaa
      841 gatcaaatat gtagacgatt agaagacatt gatgttggta ccaaatttcg cgaagacatt
      901 tgggaacgac ctgaaggcgg cggaggaaaa acacgcgtta tcgaaaacgg aaacgttttt
      961 gaaaaaggtg gcgtaaacat ttccgcagtt cacggcaagt tgcccgaaac tatgcaaaaa
     1021 atgttcaacg ttggcgaagc cgatttcttt gcctgcggat tgagtttggt tattcaccca
     1081 aaaagcccga tggttccaac ggttcatgcc aattggcgtt attttgaaat gtatgatgat
     1141 aatggtgaag tgattaattc gtggtttggc ggcggacaag atttaacacc ttattatatg
     1201 tttgaagaag acggaaaaca ttttcaccaa acctgcaaaa acgcttgcga caaacataat
     1261 ttagattttt atccaaaata taaaaaacaa tgcgatgcct atttttggaa tgcacatcgc
     1321 aatgaagctc gtggtcttgg tggattgttc tttgattatt gcaaagcaac cgacgacatg
     1381 aaaatggaag attggtacaa tttcgtaacc gaagttggaa atcctttctt agaagcgtat
     1441 gtgccgattg ttgaaaaaag aaaaaattca gcttacacag cagaacaaag aaattggcaa
     1501 gaaatccgcc gtggtcgtta tgtagaattc aatctnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnncagaa caaagaaatt ggcaagaaat ccgccgtggt cgttatgtag
     1681 aattcaatct agtgcacgat aaaggaactt tattcggatt aaaaaccaac ggtagaattg
     1741 aaagcatctt gatgagtttg ccaccacatg tgcaatgggt ttatgaccat catcctgaaa
     1801 aaggtagcga agaagaaaaa ttattaaaaa tattagaaaa accaattgat tgggtttagt
     1861 tttttaagtt ctaaacttga aacttgaaac ttgaaacctg aaacaattta aacgtataaa
     1921 ttatgttccc attacaaaga ggcagaagac taagagtaaa cgaaagcatt cgttcattag
     1981 ttcgtgaaac aagtttatct ccggcagatt ttatgttccc gatgttcatc atggaaggcg
     2041 aaaataccaa agttgaaatt ccatcaatgc caggtattta tcgtcgaaca ttagatttaa
     2101 cagtagaaga agtaaaagaa ctttttacac tcggaatccg cgccgtaaat atttatgtaa
     2161 aagtaaatga taacttaaaa gacaatacag gaaaagaagc ttggaactca gacggattga
     2221 tgcaaaatgc cattcgtgcc attaaaaaag catgtcccga aatgattgta atgcccgatg
     2281 ttgctctcga tccatattca atttatggtc acgacggaat tattgaaaaa ggagacattg
     2341 ctaacgacaa aaccaacgat gctttagtaa aaatggcagt ttctcatgcc gaagctggag
     2401 ccgattttgt tgcgccaagc gacatgatgg acggaagagt tttaagatta agacaaggtt
     2461 tagatgctgc aggttttcaa aatgtgggca tcatgagtta ttctgcaaaa tatgcctctg
     2521 ctttttacgg accatttcgt gatgcgttag attcagctcc aaaagaagcc gatgttgtgg
     2581 ctccaaaaga taaaaaaacc tatcaaatgg attattcgaa ccgaatcgaa gccgtaaaag
     2641 aagcactttg ggatgttgaa gaaggtgccg atatggtaat ggtaaaacct ggaattgcct
     2701 atcttgatat tgttcgcgaa gtaaaaaatg cagtgaatgt tccggttacg gtttttcatg
     2761 tttctggcga atatgccatg gtaaaagcag ctgccgaaag aggttggctt gaccatgata
     2821 aaatcatgat ggaacaatta atgtgtatca aacgtgcagg agcaagttta atttcgactt
     2881 attttgctaa agaagccgct attttattaa acaaacaata atgaaacgag taactttttt
     2941 tgcattaacg gtactattat tttcttgtaa aaaagaaagt gaagataatt ttggtcaacc
     3001 aaaatcagaa gcaaccgaaa agatttcagg taaagaactt tttgaaacta aaggaaattg
     3061 tgtagcttgc cacatgacaa accaaaaagt tattggtcca agtttagaag aaatttccaa
     3121 aatttataaa gacaaaaacg ctagcatagt ttccttttta aaagaagaaa gccaacccat
     3181 tgttgaccca agtcagtacg aagtaatgaa aaccaatttt gccataacta agaaaatgtc
     3241 cgacgatgaa ttaaaagcat tagaagatta tatttttgct cattagaaaa tagaattcac
     3301 tatttttata gaatggaaac ttgctttatc aattcaccac taggcatcac aaaaatagtt
     3361 ggtgatgaaa atggaatttc tgtgatttct attttagcag aaggagaagt ttctaaaacc
     3421 attcccaaac cactaaaaga atgtgttgca caactcggcg actattttga tgggaaaaga
     3481 acagaattta cgtttacttt aaatccaact ggaaccgatt ttcaacaacg agtttggcaa
     3541 gaattactta aaattccttt tggcaaaacc atttcttatc tcgatttatc caaaaaaaca
     3601 ggtgatgcaa aagccattcg agccgttgct tctgctaatg gtaaaaatcc actttggatt
     3661 gtcattcctt gtcatcgtgt tattggaact gatggttctt tgactggtta cgctggtggt
     3721 ttgtggcgta aaaaatggtt actcgaacac gaaaatccat ctaatcagca atcgttgttt
     3781 tgaattcata gattttttta tggctattgt tacatgaata agaattttat gtatttttat
     3841 gtgcataaat aaatgtctaa ttcttattga tataacttat gatagaaaaa atcaacctca
     3901 ataatattct ttttcttgat attgaaaccg ttccaataga agaaaatttc aataatttag
     3961 atgatgaaat gcaacagctt tgggaacaaa aaacacaata ccaacgtaga gatgaattta
     4021 ctcccgatga gttttatgat agagcaggaa tttgggctga atttggtaaa atagtctgta
     4081 tttcggcagg ttattttgtc aataaaagtg acattcgaaa cttcagagtt acttcttttt
     4141 ttggcgatga ggtgaagatt ttaaaagatt tttctaactt attaaacaat cattataatc
     4201 agccacaaca cgttttgtgt ggtcataatg caaaagaatt tgacattcca tttatcgctc
     4261 gtagaatgat tatcaatcag gttgccattc ctgataaact gaatttattt ggcaaaaaac
     4321 cttgggaaat tccacatttg gatacattgg aattgtggaa atttggcgat tacaaacatt
     4381 ttacttcttt aaaattatta accaaaatcc tcggaattcc ttctccaaaa ggcgatattg
     4441 atggtagtca agttggtcat gttttttatg tagaaaaaga catcgaccga attgtaacct
     4501 attgtgaaaa agatacgatt gcggtagctc aaattttcct cagacttcgt cgagaagatt
     4561 tattggtgga tgatgagata attcatgtgt aattattgaa gtaacatatg atttattaca
     4621 tttacaaaaa ttaatttttc atggttttaa gtagattttg gctcgcaatt tttatatctt
     4681 caattgtctt tgtaattatc agtttatttt cgggcaccaa ttacaccatt gattatgtgt
     4741 tgaatggtga aaaaggcaat cctattttaa tttccgaaaa atatatcgat caagttccgg
     4801 catttattcg agatagtatc caattgaaag aagataagac catgattgta aatcgtgatt
     4861 tatcaaatcc tgatacgact tatgtttata aaaataaaac ggttcaaata tacagtggcg
     4921 ttcaaaaatc ggatggttta ctggcaactt gtaaaagcag tttggtggat attattattc
     4981 cgcttattgc ttatttagca ttcttttgtg gactaatgga actgttaatt atttcgggag
     5041 tttccggaaa attagccaaa gtattgagtc ctgtttttgt aaaagtcttt ccaagtattc
     5101 caaaaaatca tccttccatt tcatatatga cgttaaattt tgcagctaac tttttaggtt
     5161 tagattcggc agcaacgcct tttggattaa aagcgatgga aagtttacaa gaaataaacc
     5221 ccgaaaaaga aaaagctagc gatgcacaaa ttatgtttat gtgtttgcat gcatcgggtt
     5281 taacattgat tgcaacttct attattggtt accgcgcagc agcaggttca agtaatccag
     5341 cagatgtgat gttgccttgt atcataactt cttttattgg aacaatcgca gcttttatca
     5401 tcgttggaat caaacaaaaa atcaatttta aaagtgcttc tttagcaata ggtttaatga
     5461 ctttgatagg tgccattatt ggtttgttga tgtatgtaaa tcatttggat cttatcggga
     5521 aaaactattt cacatctaat ctttcgggat tgatattatt gaccattatt gtttttacct
     5581 taatttatgc ttttcgaaaa gagaaattgt ttgcagaagc aaaaactacc gtttttgatg
     5641 cttttgtaac gggtgcaaat aatggtgtta aaactggagt taccattttt ccttatgttt
     5701 tgggaatgtt agccgcaatt tcattgttta gaaatagtgg tttgtttgat attattagta
     5761 atagtttagc attcgtattt tcaattgttg gattgaataa agaaataatc gatgctttac
     5821 cagttgcact attgcgacct tttagttcgg ctggttcacg tgggtttttg attgattcaa
     5881 tgaatacttt tggcgccgac tcgttaacag gaagattgag tagtatcttt caatgtagtg
     5941 ccgaaagtac gttttatgtc attgccgttt actttggttc agtaaatatt aaaaatactc
     6001 gttatgcttt aactacgatg cttttagtag atttaatctg tgtaattaca gcaatttttg
     6061 tggcaacttg gtttttttaa ttaattatta gtatcctaca aggttttcaa aaccttgtag
     6121 gtattaaata gtataaaggt aaagtatttt taacatcaat cctgcaaggt tttcaaaatc
     6181 ttgtaggtat taattagtat gaagttctgc aatttatttg tagttttgaa agtataatta
     6241 taactagaaa tgaaattaga ggttatagaa aaagaccatt tttatcacat ctataatcgc
     6301 ggaattaata gcgaattgat ttttcattcg aatgaaaaca aaagttattt tctaacttta
     6361 tttaacaaac atttacatca aaacgtagct acatacgctt attgtttaat ggacaatcac
     6421 tatcattttg tactgcaaat cactgaagag gaaaagaaag taactcaagc tttttcaaat
     6481 ttcttcaacg cttatgcaaa agcgtttaac atacaaaata gtagaacagg aagtttgttt
     6541 gaaaaacact ttaaacgaat aaaaattgaa gatgaaaatt ttttaagaaa tattattcaa
     6601 tatgttcatc ttaatccaaa acatcatttg gatattaatt acaaaacata taaatattct
     6661 tcttttcaaa ctatcctttc aaataaacct actaagttgc ggagaattga agttttaaaa
     6721 ttatttgggg atgtagacaa ttttatttat tgtcatgatt ataaaaacga tttactttct
     6781 gaaaagttta cttttgaata atcttaacac ctacaaggtt ctgaaaacct tgcaggagaa
     6841 gccgactttc cttaacttta cgcttcaaaa aaactcataa aatggacttt atatatcaag
     6901 aaccttttcc aattttaaaa gacgatacac aatacaaaaa aatcacttcc gattatgtaa
     6961 aagttgaaaa aattggtaac agagaaatat taaacgttga tccaaaagga ttggaattgc
     7021 tttctcaagt tgccatgaat gatgtttcgt tcatgttaag aacatcgcat ttacaaaagt
     7081 taaaaaacat actcgacgat ccagaagcaa ccgataacga tagatttgta gcttacaatt
     7141 tattgcaaaa tgccgtagtt gccattgaag gagaactacc tagttgccaa gatactggaa
     7201 cggcaattgt gatggcaaag aaaggcgaag atgtttatac cggcgttgat gatgccgaat
     7261 ggttgagcaa aggaattttt aatacgtacc aagagaagaa tttacgctat tcgcaaattg
     7321 ttccgatttc gatgtttgaa gaaaaaaact cgggctccaa tcttccggca caaattgaca
     7381 tttatgctaa aaaagggaat tattatgagt ttttattttt agcaaaaggt ggcggaagtg
     7441 ccaacaaaac ttttttatat caaaaaacaa aatcgttgtt gaatgataaa tcattagatg
     7501 aatttgttcg tgaaagaatt aaagatttag gaacttcagc ttgtccaccc tatcatttgg
     7561 ctttggtcat tggcggaact tcggctgaag caaatttggc tgcggtaaag aaagcatcgg
     7621 caggttattt tgacaatctt ccaacttcag gaaatatggc tggtcaagcg tttcgtgatt
     7681 tagaatggga acaaaaagtg ttgaaaattt gtcaagaaag tgctatcgga gcgcaatttg
     7741 gaggaaaata ttttgctcat gatgttcgtg tcattcgttt gccgcgtcat gctgcttctt
     7801 gtccagttgg attaggcgtt tcttgttcgg ctgatagaaa tataaaaggg aaaattacca
     7861 aagacggaat ttttgtagag caattagaag ccaatccaaa gcagttttta ccggaagttg
     7921 cgccacattt agaagcacca gttgaggttg atttaaatcg ccccatgaaa gaaattttgg
     7981 cagaattaac caaatatcca atcaaaactc gtttgaaatt aaacggtact ttaattgtgg
     8041 caagagatat tgctcatgca aaaattaaag agctacttga tacaggaaaa caaatgccgg
     8101 aatatttcaa aaatcaccca atctattatg ctggaccagc aaaaactcca gaaggaatgc
     8161 catcgggaag ttttggacca acaacagcag gaagaatgga tgtttatgta gaagagtttc
     8221 aaaaagctgg cggaagtatg attatgttag ccaaaggaaa tagaaccaaa gaagttacca
     8281 atgcgtgtaa tacatacggt ggattttatc ttgggtcaat tggcggacca gctgcaatct
     8341 tggccaaaga aaacattcta aaagtagaag ttgttgattt cccagaatta ggtatggaag
     8401 cggttcgtaa aattgaagta aaagattttc cggcattcat catcactgat gataaaggaa
     8461 atgatttctt cgctaattta taaaaatgga tttcaaagaa accttttcgg agttttattc
     8521 aaaagtaaaa aacagtattg ctgatggtac ttttgccaaa ttaactttgg caaaaacgat
     8581 tggaaatact gatttaatga acatttatgt tcgtccgatt gttgatgaag atgttttaaa
     8641 actagaattg aaattcaaat tccaaaaaga agaaataata gaaataagaa ctattgaaga
     8701 aacctttgat cgtttattgg aatttataaa caacccgttt ttatcggcta ttttgtttac
     8761 aaccgcgttt gatttgactt ataaattgaa taaaaaaaga gctgttagta tgattgaaca
     8821 attcccaagt tttggtaatg caaatgaagt tttgttggaa tttttggcta ataagtaaca
     8881 aataaaaaaa ataaaaaaaa ccacaaaatt ctaatgagtt ttgtggtttt ttgtttcgta
     8941 taaatgagtt agtcaataat catttcagga atttcgcctt caatgattag ttctgcttcg
     9001 gttgaagcaa taatatgttc aacggtaaca tctggcgcac gttctactaa tttaaaacct
     9061 tttggagtaa tttccataac ggctaattcg gttactactt ttttcacgca accaacgcca
     9121 gttaatggca aagtgcaacg ttttaaaatt ttgctttcac ctgctttgtt tacatgcatc
     9181 atggcaacga taatgttttc tgcggaagcg actaaatcca ttgctccacc cattccttta
     9241 accatttttc caggaatttt ccaattcgcg atatcaccat tttcagcgac ttccatcgca
     9301 ccaagaatag ttaaatcaac tttttgagaa cgaatcattc caaaactaaa ggcagaatca
     9361 aaaaaacttg ctcctggtaa ggttgttatg gtttgttttc cagcattaat aatatcggca
     9421 tcttcttcac cttcataggg aaatggtccc attcccaaaa cgccattttc agattgaaat
     9481 tctactgata tatcggtacg aacataattg gcaaccaacg ttggaattcc gatgcctaag
     9541 tttacatagt aaccatcttt tacttctttg gcaattcgtt ttgcgatttg atttttatct
     9601 aaagccattg tatttgattt gaaaatttga tgatttgaaa attaaccttt ctttgaggaa
     9661 gatataattt tagaaattat caaatttata gattgtaatt tttttaataa ttcttcgcta
     9721 ggatttgggt agaattcgga attcaaacaa aggtctaacc aatattgtaa ttcatctgtt
     9781 tctttagctg agattttaaa tttatgaata aaatctgctt tactttccgc attctgagct
     9841 tctctgatat ttgcgcctat tgaagttcca cttctaaaaa tttgagatgc catttcaaaa
     9901 cgatttgttt ttctaatttc ttcagaataa ttaataattt ctaaagcaaa ctcaaatgtt
     9961 aattttacaa ttaaattatc tttatcgttt ctcatcttca aattttcaaa ttaaagaatc
    10021 ttcaaattat ttttgtctaa cggttcgttg ttcaattctt ttttcaaatg tttcaccttg
    10081 aaaaatacgt tgtatcataa ttccaggaat gtgaatttga tttggatcca atgaaccaac
    10141 aggcaacaat tcttctactt cggcaatagt gatttttgct gctccagcca ttggtgcatt
    10201 gaagttacga gctgttcctt taaaaattaa atttccagct tcgtcacctt tccatgcttt
    10261 tactatagaa aagtcagctt taaaagcatg ttccaaaacg tgcatctttc cgttaaattc
    10321 tctggtttct tttccatcgg ccacttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnctt tttttactaa ttcagcaata ctattttctg gaattccaca aaggccaaaa
    10501 ccaccaagca tgatggtcat gttgtcttcg attccttgaa gtgcttcggt tactgaattt
    10561 acttttttgt taatcattgt attttatttt ttagccccga tagtagtgaa aatccttttt
    10621 tgaaaaaaaa gattgtaacg gatagcggga aatagcttct aaaaattata atccaaattc
    10681 gtctgtgtct tgctcttttt gtagttctaa ttctagtgaa tcaactactt ttggagccca
    10741 acaatctact tttatagata gattttttgg tcgttcgaat tcatcttttg acacttgtaa
    10801 atctttatct tcataacatt ttttcataaa atatccccaa attggtaaag cagctgttgc
    10861 accttgacca taagtaatac ttttgaaacg agccgaacga tcttcgcaac ccacccaaac
    10921 accagaaact aaattaggaa ccattccgat gaaccaacca tcagattgat tttgtgaagt
    10981 cccagttttt ccagcaattg gatttgtaaa tgcgtaggga taacctgtta ctctttcgta
    11041 gccatatccg ccaccttgag tacgcaatct tgaaccagaa ccaccttcag tcacaccttc
    11101 taataatttg attactgcaa atgcgatatc tttattcaaa acatcatgtg attcgggaat
    11161 tggctcataa agaacaacac cgtttttatc ttcgattcta tcaatgaatt gaggtttgat
    11221 gtaaacgcct tgattggcaa aagtactgta agcagcaacc atatcttcta cagtaacttc
    11281 cacagcacca agcgcaatgg atggttgtag aggaatttcg ctttttacac ctaatttttt
    11341 tgttaaatta acaacagctt ctggaccaac tttatccata agtttagcag aaactgtgtt
    11401 tatagaattg gctaatgctt ttttcaaagt gaccattcca cggtatttat tgtcggagtt
    11461 ttttggcgtc caagctgcag ttacatgatg tcttccaaca ggaattgtat agggactatc
    11521 gattatagaa tcgcaaggcg acatacctaa ttgttcgata gctgtggcat aaacaaaagg
    11581 tttaaaggtt gaaccaacct gtcttgcacc ttgaccaacg tggtcgtatt gaaaatattt
    11641 ataattgatt ccaccaaccc aagctttgat atgtccagtt tgaggttcca tagacatcat
    11701 tccggcttga aggaaggatt tgtaatatct aatagaatct aaaggcgtca ttattgtgtc
    11761 tttttcacct ttccatgtga aaacacgcat tttggttttt ttgctgaatg aagcgataat
    11821 ctcatcatct gttttatcta aatcttttaa aatatgccat ctttcggaag atttcatagc
    11881 acgttttagg attctattcg tttcatctac ggaaattttt aagaatggag catttttgtt
    11941 ccctttttgt tggatgaaaa attcctcctg aagattcttt atgtgtgctt gaacggcttc
    12001 ttcggcatgt tcttgcattt tggaatctat agtggtataa atttttaaac catcggtgta
    12061 aatatcatat tctgttccgt ctggtttttt attttctttt acccaatttt tcatgtattc
    12121 ccgaagaaat tctctaaaat acgttgcaat tccttctttg tgtgtttgta atttgaaatc
    12181 cagtttaata ggtaatttgc tgtaactttc tttctgagct tctgtaatta tttttgatct
    12241 aaccatttga cccaacacta cgtcacgtct atttttaaca ccttgtgggt ttctaacagg
    12301 attgtaaagc gacgaatttt tgaacattcc aactaaaata gccgattcat caatggttaa
    12361 atctttgggt tctttattga aataggtttt tgaagctgaa cggattccaa tggcatagtt
    12421 gttaaaatca tatacattaa aatacatcgc aattatttcg tttttagtat attgtcgttc
    12481 aagtttaata gcaattatcc attccttaac tttttggact aatctaaaaa tcttaaaagt
    12541 ggaaccttct ccatgaaata attgtttggc taattgttgt gttatagtac ttgctccacc
    12601 atcggcacca agttttatag cggcaccaaa agttcttcga gcatcaattc ccgagtgttc
    12661 ataaaagcgc tcgtcttcgg tagcaattaa agtgtttacc aaatgtggcg gaagatcttc
    12721 gtatttaatt ggggttctat tttcgttgta aaatttccca atcgttacac catctgacga
    12781 aataacttcg gtagccaagt ttgattcggg attttctaat tcttcaaaag tcggcattgc
    12841 tccaaaaaat ccccaagatg ccaaaaggaa aaatagaatt aacgcactaa atccataaat
    12901 aaaaaattgc caaaattttc gtttatagta cttaatgtca ttcgttgtgt ttttcttatt
    12961 ttgagccata atatattttt attctttaga ttctattcta aaaccaacat ctgtaattcc
    13021 tttgagttgt tctactcctt caatttttcc tgtttgtcta acggcttgta tgatatggat
    13081 tttataaaca cctttttggt caaatttttc attggcttta taaaaaagtt tattttcttt
    13141 aatgtcacta aatccttctc caagaagtgt accatctgga tgggtcataa catattctag
    13201 agtatctact ttaatttttt tgttaggttg ctctaaagaa acaatcaaaa acaagttgtt
    13261 aaatggataa tcatcattgt ctcttacatt caaaaacaaa ttatacgttt tatctttttg
    13321 aagttcaggc aattcaaaag taacaacact atctttatgc cacgaatttc caactgattt
    13381 gtattcgtca aaaactcgat tcttatcaca agaaacaagg cacatgcaga taaataaaag
    13441 gactaaacta ttttttaaca tcatttttag aaataatgat tggttttttc tttgaattat
    13501 tcgtgttgtt cgaattgttt tgattacttg gttttttctt tttgttgttt ttgtttttcc
    13561 ttttgggttg atcaaaacgc gttaaacttt cttgacccat agcattattg aaatcttttt
    13621 caggtagcgg tgccatttct aatgcaaaat cttctaacga agtagctcgt tttttttctt
    13681 tatttaaagc tataatttct tttacatgtt caatttttaa cacgtgccaa ttggcaaaat
    13741 tatcggtgta agcaaaccac attaaacctt taaaaatatc ttgtttttga caaatagcat
    13801 cccctttttc ggtatgtaat ttggtttcaa actccggaaa gtcttttaaa gcatcaaggt
    13861 aagtatcaag ctcatagttt aaacagcact ttaattttcc gcattgaccc gctaattttt
    13921 gtggatttaa tgaaagttgt tggtatcgtg ctgccgaagt attaacactt ctaaaatcag
    13981 ttaaccaagt agaacaacac aattctcttc cacaagaacc aataccgcct aaacgagctg
    14041 cttcttgacg gaaacctact tgtttcattt cgattctggt cttaaaactt tgtgcaaatt
    14101 ctttaattaa ttgacgaaaa tctactcgat cattagcagt ataataaaaa attgctttgg
    14161 acccatcgcc ttgaaactca atatccgaaa ttttcatttc taagttgagt cggatagcaa
    14221 tttctctggc ttttactttc atcggttctt ctttatctcg ggcgtcgctc caaatatcaa
    14281 tgtctttttg agatgctttt cggtatattt taggcacttc gcctttaaaa tcaactcctt
    14341 ttttcttcat ctggatttta accaattcgc cagtaagagt aacaataccc acatcatgac
    14401 caggagaggc ttcggttgca actacatcac caattgataa tgtaattttc tcagtattgc
    14461 gataaaattc ttttctacca tttttaaatc gcacttcaac gcaatcaaaa gctacttcac
    14521 cattgggtaa actcatgttt gaaagccaat cgaaaaccgt taatttattg caactatcgg
    14581 tgccgcaagt cccgttattt ttacatccac gtggcgtacc gtctttggta ctagtggaac
    14641 aatttgtaca tgccataatt ttgaatatat attggtcatt ccgaaaaaaa acatcggaac
    14701 tactatcata aaacgaataa tttgtaaaga tagtattttg ttagttaaaa tttcaaaaga
    14761 aatttaacct aaaaaaagta gcctagttta gctcaaccga ataatttagc atatttctat
    14821 tgggtttttc taaatttttt ttagaaagaa atatgttttt gaataaaatt ttattccaag
    14881 aattacgttt ttgccattct gaaaaggttt cagttttcca atcaaaatcg tccttccaat
    14941 agtattttgg agaaacgtaa cagtaggtat atggaattaa ataagagctg tcattctctg
    15001 ttttaacttt taaagattgc tcttcgtttc gactaattgt tttgggagaa agtaaagtaa
    15061 tgcctctttt ggaagcttct ttttttagaa atgtatttat ttcttgataa tcgttttcaa
    15121 ttgcttttaa actgaaatta ttataggttg tttctccaat tttatcaata ggtcgaagtt
    15181 gaataatgtc tatagaatac ttatcataaa ccttgaaaaa ctcttttaat tcatgaaaat
    15241 tatcgacgtt aaaagtgtaa tttattcgta atcgtaacgt tggattcttc tttttttctt
    15301 cggtaatgta acctaaaacg gcatggaatt tttcgaaaac acctttatcc atcaaatctt
    15361 catacgtttc cttgaaaacg ccatgcatag agaaaataaa ttcatttaaa ccagcttgag
    15421 atagatcaat aatatcttga ttttctaata aatttccatt ggtaaccata ctaatatatg
    15481 gaactttgtg ttttttggca attttgatta attgaacgtt atgcttaaaa agcgttggtt
    15541 ctgcgccaca accaatttgt agttttagtg cgtttttaaa atttactttg gctaattctt
    15601 ctaaatcatt ttctttaaaa atgcctttca tgttggttct aacgtattca tcatcggtaa
    15661 aataacacat tttacaacga agattacagg ctaaaaccgg atcaaattga atggcaaggt
    15721 atcgtttatt taaaacactc aataaccata aaccaaagaa ctttattcga tgacttttga
    15781 tagcatgatt gaatttgagc agtttataaa tatccatttt ttctaattag tttaccaatt
    15841 taaacaattt atccgacaaa cgaactctaa aaggaacttc aaaagtgact ttatcaccaa
    15901 tttttgccgc tgaattttca cagccgttga caaacatttt ttctaaaatt aactcttgat
    15961 ttcctgtagt tggaccagaa attagaattt tatctccaag ttgcaattcg ttgttttcaa
    16021 tgatgaataa tccaattcca gcttttacaa agtaatgttc ggctttgcca agaataactt
    16081 tttttgcttt tatcttttga cgaatgtctt ttgttttttt ggcaacagcc aaagccacat
    16141 cagataattc tcctgaatgt ttgaatttta atttttctga ttttcctttt ctaaaaactt
    16201 tgttcccaac ttgtttgcca cttcttaatc gaacttgttc ttccagggat aaatgtgtaa
    16261 tgtctaaaca ttcagtagaa cagcaatttt ccattacagc ttgacattca tcacattgaa
    16321 taaatagcaa atgacaacca tcattagcac aattggtatg attatcacaa ggtttaccac
    16381 attgatgaca ttgagcaaca atatcatccg taattctttc ccctaaacga ttatcgaaaa
    16441 cgaaattttt accaataaat ttactttcta aaccttcttc tttgatttgt ttagcataat
    16501 tgataattcc gccttccagt tggtaaacat ttttgaaacc ttggtgtttg tagtaagcag
    16561 aagctttttc gcaacgaatt ccaccagtgc aatacatcac taagtttttg gtttccttat
    16621 aatcttttaa ttgctcgttg atgatgggca aactttctct aaatgtttca acatcaggag
    16681 ttatggcgtc tttaaaatga ccaacctcgc tttcgtaatg atttctaaaa tcaactacaa
    16741 ttgtgtttgg atcttcaaga atttcattga attcttttgc tcctaagtga attccttttt
    16801 tggtaacgtc aaaagtttca tcatttaaac catcggctac aattttatga cgaactttta
    16861 tagttaattt taaaaacgaa tgatcatctt gttcaacagc aacattcaaa cggatgcctt
    16921 tcatgaaatc ataaacttct agtgtttcac gaaattcttc aaagttatca gcaggaacgc
    16981 tcatttgagc atttattcct tcgtgagcaa cataaattct gcccaatgca tcgagtgcat
    17041 tccaagctaa gaataaatcg tcacgaaatt tttttggatc ttcaattttg gcatacgcat
    17101 agaaagacaa ggttagtcgt tgttttccgg ctaaatcaat tagctctttt ctttcttctg
    17161 cgcttaaggt gttatacagt tgcatgctat aaacgtttaa gtgagaaata tgtttttttg
    17221 aactctaatt ggaagaattc gtttgcaaat ttaatgctta accgcaaagt tcgcaaggtt
    17281 tttttacgca aagttcgcta tgattaaaaa ataaccacaa attcacagat tattaataaa
    17341 atctgtgaat ctgtggctaa aaatattctt ttaagttctt aacagaattc agcgtaagca
    17401 tcttttaagt tttcagcaat catttcagcg ggtcttcctt cgatatgatg gcgttctaac
    17461 atgtgcacca attcaccatt tttgaacaac gccatacttg gcgacgatgg aggaaaaggg
    17521 aacatttctt ctctagcagc gtcaactgct tctttgtcaa cacctgcaaa aacggttact
    17581 aaatggtctg gttttttgct tccgtccaaa ctcatttttg ctccaggacg agcgtttctt
    17641 gcggcacaac cacaaactga gtttactaca actagtgttg ttccttcttc tttgatcgca
    17701 ttttttactg cttcggcact atataaatct tgaaaacctg cttctgaaag ctcagctcgc
    17761 attggtttta ccatttcttc tggatacata atattttaga ttttagattg ttgattacag
    17821 aaatttgatt taattaaatc attgcaaaga tacaacgaat tctaattttt tattttaaat
    17881 aaatcataaa gaattgttat aatgaaatag aagattaaaa tgtaaaaatc cttccaaata
    17941 acgctttaat gaatggaatt tttggacatt tccaaatcac ttgcaaatcc tctaaaaatg
    18001 tagtttcttc atctaaaaac ctaaaaatta aggttggatt tcctttttta aataacgacg
    18061 aaaacactaa agaacctaat gcgttatttt gaaataaaac atcaataaac aataaatcat
    18121 acagccaata tttagttttt ttgtgaagtt ttctaaagtc tgtttcattg tgcgaatttg
    18181 aatttttagt ttctaaaaac gctaccaatt cttttgattt tttagttaca tttttaaatg
    18241 taaagcctgt acttgctttt gtccatccgc cagctgagcc aatgtttaca atgtttttag
    18301 tattgttttt ccaaaattta tagcacgtca tgggtatgtt tccttgttct ttttctacaa
    18361 tttcaaattc agtaactccg agtttttcga tataattttt tatttctttt tcgtattcgt
    18421 ttttagaaag taagtctttt gaaaacaaag tatattctag taaagcttct gtttcagatg
    18481 ttggtaaaac atacataaaa cgagtgttgc ctttttgttc cacagaaaaa tccataaacg
    18541 tggcacaatt tggattgaaa acagcttctt tacttttgat gaaccatcct ataaaatgtt
    18601 gttgtaatag tggatatttt gattgatttt ttataggctt tggattgaaa atggaattaa
    18661 aaatcttgtt acaagtataa ttatgggttt ctgtttttaa aacacagtga tttcctaatt
    18721 cctcaaaata aacaactttt tgattgctaa agtgaatgtt atcttgtttt gaaatcaatt
    18781 cgaaaaccat gttataaaaa tttaatccac gaaccattgt atattggtat gggtttagat
    18841 ttaaaacacg tttataagtt gcatttttaa ataacgcttc attccacttt ttggaagtta
    18901 tttcatcaaa taaattagta tcatcccaaa aacaccacgt tctgtcgtta ttcttttttg
    18961 aatctatgtc taataaaaga atggatttgc cttgaaaatc ttctgaacga atcatttcac
    19021 aaaccgtcat taaagcggaa agtccggaac cagcgaatat ataatggtaa tgttgcattg
    19081 ttcaaaaata catttttaac gttatagatt ttgataatag tagtataaaa actaattttt
    19141 aataaccaac caaaaaaaat cttttttaat taactttaca taaaaatata aaattatgtt
    19201 atcaaaaaat atcgaagtgg cattaaacaa acaaattaga attgaagcag aaagttcaca
    19261 aacttattta tcaatggctt gttgggctga agtaaatgga ttagaaggaa tttcaaattt
    19321 catgtatgca caatcagatg aagaaagaca gcatatgtta aaactcatta aatatgttaa
    19381 tgaacgtggt ggacatgcta aaatttcaga attaagcgct ccaaaaacag agtttgataa
    19441 ttttaaaaac atgtttgagt tgttgtacaa gcacgaactt tttgtttcag aatcaatcaa
    19501 tgaattggtt agaattacgt ttgaagaaaa agattattcc acgcataatt tcttgcaatg
    19561 gtatgtttct gaacaaatag aagaagaatc acaagccaaa acaattttag ataaaatcaa
    19621 tttaattgga gatgataaag gcggattgta tttgtttgac agagatattc aatcaattgc
    19681 agcagctaat tctgcttcga atgttaaaaa ctaattgtta aaatttattt agaatagata
    19741 aaaataaact acttttgagc tgtttttaaa tttagaaact ttggcttcaa aagtaaaaga
    19801 taaaaaaaag aaaaaagaca agaaaaaaga ttcaatcgat gtgttgatac ttttaaaaaa
    19861 tcttgatgcc tgcaaatcta gttgttgcga aaagtataaa aaaaatgaaa aaaaacggtg
    19921 caaacgttgc ccgatgtttg accttttaaa atccataaac taaatgtttc caaatattgg
    19981 aagcattttt ttataatcta aatgaaaacc caagagcaaa atagttatca ggtgcatttt
    20041 ctgaaattcc aaatccacta gacaaatcta gcataaaatt agtgctaatc aaataggtaa
    20101 taccaccatc aaaattatga tttgcttcat tgttttgagg agcaaatcca aataattcaa
    20161 tataagagcc taatttttct gtaattgaat aacctgttgc aacagtataa ataaaagttg
    20221 gttcaggtgt aaaaccatcc cattcagcac caagattata acttaaagag aatttttcac
    20281 ttaaagtatg ttgcattaca aatagaaatt caggtgcaaa ataatctgtt ataaaagcag
    20341 ttgaagcaac attgggcaat gaaacatgac caataaaaga agtttttggg aaaacaccat
    20401 tttcttcggc tatttttatt ttgcaaccta ccaaaatcgg attaaaacca gttattgttt
    20461 ctacattaaa cttttctgag gcaaattcag taattaatcg taattcaaaa ttttcattaa
    20521 aaccatattt ccacaaagtt gaaggcaaaa gatttgtttt actattgaca tcattttttt
    20581 gaaaagaaaa tccagtttct acttgaagca ttcctttcgg agttaaagaa ggtgtttcag
    20641 tttgatctgg tctgtcagtt tgaataggtt caatttcttt ttcttgagca aatattgttg
    20701 aatacaaaaa caaagcaaag aataaaattt ttgatttcat aatttttaat ttagacaaaa
    20761 ctaaaaatat ttttcacgat acaaaaatta ttgctacttt tgttaaaaaa acaggcaaat
    20821 gacaaattca gaagaaaact atttaaaggt aatctatcat ttatccttag tttctccaaa
    20881 aggtgtaaac actaatgcca ttgctggaat gcttgaaacc aaagcttcct ctgtgacgga
    20941 tatgatgaaa aaattagccg aaaaagaatt ggtgctttat caaaaatatc aaggagtaac
    21001 tttaaccgaa aaaggttttt tatcggctaa aatgattgtg agaaaacatc gtttatggga
    21061 agtttttttg gtagaaaagt tagattttgc ttgggatgaa gtacatgaaa ttgcggaaga
    21121 attggaacat ataaaatccg aaaatttaat taataaacta gatgaatttt taggttttcc
    21181 agcctttgat ccacatggtg atccaattcc aaatgaaaat ggagaaattc aaaaagtatc
    21241 caagttgttg ttgtccgaat ctgaaataaa cagagaatat cagtgtgttg gtgtcaaaga
    21301 ttcttcttcg gattttttgc aatatttaga caaacaaaat atagcattag gttcaaaaat
    21361 aaaagttatc aataaggaac agtttgatga aactttaaca atagaagtaa ataatacatc
    21421 attgaccatt tcgaataaaa ttgctaaaaa tttatttgta aaaaataaat cactatgagt
    21481 aaatcattag aagaagtaaa cgaatcaatt gctactcaag gcaaaaaatc aacttttaga
    21541 aaaattttag cattttttgg tccggcttat ttgatttctg ttggctatat ggatccaggg
    21601 aattgggcaa ccgatttggc aggaggaagt cagtttggct atgccttaat ttgggttttg
    21661 ttaatgagta atatcatggc attattactt caaagtttga gtgcaagact tggaattgtt
    21721 acacagcgcg atttggcgca agcttcaaga gaaacctatt caccatttat taattacata
    21781 ctttatttct tagccgaaat agctattgca gcttgtgatt tggccgaagt tcttgggatg
    21841 gcaattgggt tgaatttatt gtttgacatt tccttaatta atggagtatt gattacggtt
    21901 ttagatactt ttttgctctt gtttttaatc aacaaaggga tgcgaaaaat ggaagctttt
    21961 attattgcat tagtagctat aattggattt tcctttgtgt ttgaaatgat ttttgccgaa
    22021 cccgaagttt caaaaatatt tgcaggatta gtacctagct tgcctggaga agccgcattg
    22081 tatattgcca tcggaattat tggagcaacc gttatgcctc ataatttata cttgcattcc
    22141 tctttggtac aaactcgaaa atttgataga agtgatactg gaattaaaca agcattaaaa
    22201 tataattttc ttgatagtgc gattgctttg aatttggcat tttttgtaaa tgctgccatt
    22261 ttgattttag cagcagctac tttttataaa aatggtatgt ttgaagttgc tgaaattcaa
    22321 gatgcacaca tgttgttaga accactttta ggaacacatt gggcaccaat tttatttgca
    22381 gttgctttga ttgctgcagg acaaagttct acaattactg gaactttagc cggtcaaatt
    22441 attatggaag gctatttaaa tttgagaatt cagccgtggg tcagaagaat aattacacgt
    22501 ttaatagcta ttgttccggc tgttgtaact atattatatt ttggtgaaag tgttacagga
    22561 aaattgttga ttcttagtca agtgattttg agtttgcagc taggttttgc tattatccca
    22621 ctaatacatt ttgtgagcga taaaacaaaa atgaaaggtt ttcatattgg aaaagcaaca
    22681 caaattgctt cttggttaat tgctttaatt attgtttctt taaacggaaa attagtttat
    22741 gatgaaataa aaggttggtt agaaacagct gaaaatccta cagtcatttg gtttacggta
    22801 gttccgttaa ctataggttt tttgttctta ttactcttta ttattttcaa accgttcatc
    22861 acgaaaacta aaaataatat tgaaaatcat tctccacatt ctttaaaatt aaactttgag
    22921 gaaacaaaaa ttaatgataa aaaagacatt gctgtttctg ttgatttttc cacagctgat
    22981 gaaattgcac taaatgcagc aattgaattg ggaggaagga atgctaatta tactctaatt
    23041 cacattgtag aaaccgttgg tgctatgata tatggtgaaa aaactgatga ccacgaaacg
    23101 acgattgacg aaaaattact tttggaatac caagaaatgt tgaaggaaaa aggatttaat
    23161 gtctccacaa aattaggatt tggaaagcca tttaaagcaa ttccaacaat aataaatgaa
    23221 ggtaaatatg atgttttagt aatgggaaca cacggtcata caggtattaa agatttgctt
    23281 tttggcacaa cggtaaataa attgcgacac aaaatttcga ttccattatt gattgtgaaa
    23341 aattaatgac aaccacaatc gtctttgtca caaccttttt ttgattttgg cttccaaata
    23401 aattttgtca ctaaaaaacc aatagcaatt cctaaagcta tataaactag tatttcttgg
    23461 acattcatag gttttatttt aaaagttgat aggcaattaa tgctgtgata taggcaaatc
    23521 cactcataaa aaccaactga ataattggcc atttccagga gttggtttct tttttaacaa
    23581 tagctaaagt agaaacacat tgcatggcaa aagcatagaa taataataat gaaattcctg
    23641 tagcaaaatt gaatattttg gagccatcgg ggttaatttc ggcttccatt cttttcttaa
    23701 tagtggtttc ttcatcgcta tgactgccaa cactatagat agttgctaat gttccaacaa
    23761 aaacttcacg agcagcaaaa gagcttacaa ccgcaattcc gattttccaa tcgtaaccca
    23821 aaggttttat gactggttcc atactttttc caataattcc gatatacgaa ttttccaatt
    23881 tatattcggc aactctatca ggaattacag atggatttat ttttaaatca ctattataca
    23941 cttctttttc aataattttt tgtgcgtttt taaattcagt atttggacca tgcgaaccta
    24001 aaaaccaaag gacaatagat atggcaagta ttatttttcc ggcaccaaaa acgaatgctt
    24061 ttgtcttttc aattacatta atggcaatat ttttaaacat cggaattttg tagctcggca
    24121 tttcaaccac aaaataagct ttcgttttca attttaatat tttatttaaa atgaaagcag
    24181 aaaatatagc cattccaaat cccaaaagat acaaaatcat caatgttgca ccttgtaaac
    24241 taaacaatcc taaaattctt ttatcaggaa taattaatgc aattaatatg gcataaacag
    24301 gcaatctggc agaacaagtt atgaatggtg ttactaaaat ggtaatcaat cgttctttcc
    24361 agttttcaat gtttcttgca cccataatag ctggtatggc acaagctgtt cccgaaatca
    24421 atggcacaac acttttgcca gagagtccat aacggcgcat aattttatcc attaaaaaaa
    24481 ctactcgact catatagcca ctttcttcaa gtattgaaat aaaaagaaat aaaaatgcaa
    24541 tttgtggaat aaaaatcaca ataccgccta tacctggaat gattccttcg ctaattaaat
    24601 tggtaaactc tcctggtgca agatggtttt ttgcataggt tgaaaaattc gcaaatgtac
    24661 tatcaatcca atccatagga acacttgacc aatcaaaaat agattgaaaa ataagtaata
    24721 aaatgccaaa gaaaataaga taaccaaaga ttttgtgcgt caaaattcta tctaattttg
    24781 ctcttatatc ggttgctttt gaaacgtcaa ttttttgacc tattttcaat gtatcattaa
    24841 taaactgata gcgttttatg gtttcctttt gttgtaatct ttttaaatca gaatgtgatt
    24901 tggtaaacga acttcgaatt tcatttctgt ctaaattcaa aaaattcaca tcttgtgtaa
    24961 tgaccaacca aagtttataa accaattgat ttggaaatgc atttcgcagt ttgtcaaaat
    25021 actccgcatc aatagtcgaa gcattcaagc aaggttcggt tgatagattt ttataatcaa
    25081 caatggcatt ttttaattcg gatattccga tgccacggcg agtacttact aaaacaattt
    25141 tagttttaag ttgttcttca agcaaaggaa tatcgagtga aattcctttt atcttcattc
    25201 tatcagccat gttaatgact aaaattgtag gaatttctaa atcttttatt tgagtgaaaa
    25261 gcagtaaatt tctttttaaa ttttcaacat cagttactac aacgcagaca tctggaaata
    25321 atttatcgtt tttattaagc aataattcga tgacaacatt ttcatcaatt gagctagcat
    25381 taaggctata cgttccaggt aaatcaagaa tatttgcttt gatattgttt ggcaatttgc
    25441 aaaaaccaag ttttttatct accgtaatac ctggataatt tcccacttgt tggttcaaac
    25501 ctgttaactg attaaaaacc gaagttttcc cagtattcgg atttccaatt aaagctacat
    25561 taatgttttg atggcttttc atggattaga aattaagtaa ttccacttca atttctgaag
    25621 ctgtttctaa tcggatggca agatgcgaac cattatttac attcaaataa atgggatcgc
    25681 caaaaggtgc aatttgaaca acttctaatt catttccagg taaacatccc atttctaaca
    25741 atttcaatgg aattttatcc acgtctattt gtataatagt tgctttttga cctcgtttta
    25801 ataaatttac agtagttctc aatgcttatt tagattgaat aaatttaacg caaaagtaat
    25861 gataatttat agaatacgaa ttgattttaa tcatttgatt ataaattatt cgttatccga
    25921 tagccattta atgtcgtcaa ttagtttttt gatttcatct ggattagtac cgtcataaaa
    25981 acctcgaact cttcttttac gatctactaa cacaaaattt tcggtatgaa ccatatcgta
    26041 aagttcttct ggttttccag tttttacagc taaatatgat ttcctagcaa tgaaataaat
    26101 gtctttttta tcgccggtaa ctaggttcca tttactatca attacgcctt tttcaatggc
    26161 atattttttc aaaacggaaa cactatcgat ttctggtgta acggtatgtg aaagcaacat
    26221 cacttttggg ttattcaaaa ttgctttttg cacatccacc atgtttgcag tcatttttgg
    26281 acaaatagat tgacaagtag taaagaaaaa atcagcaaca tatatttttc cttcatagtc
    26341 tttttgagta acagtttttc cattttgatt ggtaaaacta aaatctgcaa ttgtatgata
    26401 tttttctaca tattgtaccg tactatcaac cagttcagga ttgacatcgg caggattata
    26461 tattggtaat gttttttttg gctttaaagc actataaaat aaggaaacta caatggctga
    26521 aaaaaccaaa acaccaataa aaaaccatct atattttttg aaaaaggaaa gcattttttt
    26581 tgcaaaaata caaaacaaag aagcattttg taacaattat ctatttatca cgtcatatat
    26641 ttttgttaat ttaaacataa ttacaagatt taaaaataaa tttgtaaaac aaacaaaacg
    26701 caaatagata tgatttcaaa atcaactaaa atgattgtta ttttaattac tgcttttggc
    26761 tttaatcaaa ataatgcaca acaaaatgga gtaggaatag atttgtcatt aatggatact
    26821 tctgttagtc caaaagatga ttttttcaat ttcgtaaacg gtacttggtt gaaaaacact
    26881 gaaattcctg ccgacagaac gcgatgggga agttttgacg aattaagaaa aaaaaccgat
    26941 gcagacgcat tagctatttt agccgaagct tctaaaaacc ctaagtataa atcagatact
    27001 gatcaaggaa aagcaatcaa tttgtataaa tcaattatgg atactgttgc cagaaacaaa
    27061 gctggaatca aaccattatt gccgtatttg actaaaataa atgccgtaaa gaatgtgtca
    27121 gatttacaaa atttaatgat tcaaatggca tctaaaggcg gtgttggttt ctttggcatt
    27181 ggtgttggaa ctgatgccaa aaatagctct aaaaatgtcg tttatgttgg tccaggaagt
    27241 ttaggtttgc cagatagaga ttattatgtt tctgatgatg cagattctaa ggagaaaaga
    27301 gaaaaatatg ttttgcatat agctagaatg cttcagtata ttggagaaaa acctcaagaa
    27361 gcaaaaattc atgccgaaaa aatattggca ttagaaacag aaatgtcaag accaagattg
    27421 gacagagtag aacgtagaga tagaagaaaa tcttacaatc cgatgacaat tgctgatttg
    27481 caaaaaatga caaatgctat caattgggaa aaacaaatta ctggaattgg attgccaaaa
    27541 gttgatactt taattgtttc gcaaccaaaa tacatggcag cattacaaga tattctaaag
    27601 gcaaacaaag ttgaagattg gaaagcctat atgcgttgga ctgccttaaa tagcgcagcc
    27661 gataatttat caacggatat agaaaaagca aattgggatt tttatagtaa aacgttaaac
    27721 ggagccatta aacaacgccc aagagatgaa ggtgctttgc aagttgtaaa tggtgctact
    27781 ggtgaagctt taggaaaatt atatgttgaa aaaatgtttc cagctgcggc aaaagaaaaa
    27841 gctcaaaaaa tgattaaaaa cgttattcgt gcttatgaaa ctagaattaa taatttatct
    27901 tggatgtcgc ctgataccaa gaaaagtgct gttgaaaaat taaacaaaat gaccatcaaa
    27961 attgggtatc ctgataaatg gaaagattat tctgctttaa ccattaaaag tccagccgaa
    28021 ggaggaacct attttgaaaa ctcattatta atttctgaat ggagttttaa aaaaaatatt
    28081 gaaaaactga gcaaacctgt tgacaaaaca gaatggggaa tgtcgccaca aacggtaaac
    28141 gcatatttca acccatccaa taatgaaatt gtgtttccag ccgcaatatt acaaccacca
    28201 ttttatgact ataatgcaga cgaagctgta aattatggag gaattggtgc cgtcatagga
    28261 cacgaaatct ctcatggctt tgatgatagt ggtgcgcgat acaatgctga cggaaactta
    28321 gttaactggt ggaccgacga agatttgaaa caatttacag ctcttggaac agctttggct
    28381 gaccagtatt cggctttaga accattacct ggaattaaag tggatggtaa atttacatta
    28441 ggagagaata ttggcgattt aggaggaata aatgccgctt atgacggact acaactgtat
    28501 ttaaaagaaa acggaaatcc aggtaaaata gatggattta caccagaaca acgattgttt
    28561 atttcttggg caaccatttg gagaagtaaa atgcgcgatg aagccatcaa aaatcaagta
    28621 aaaactgatc cgcattc
//
LOCUS       DAOOBH010000016        28633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_2148_length_28633_cov_8.274197, whole genome shotgun sequence.
ACCESSION   DAOOBH010000016 DAOOBH010000000
VERSION     DAOOBH010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 28633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28633
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2148_length_28633_cov_8.274197"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /gene="hemE"
                     /locus_tag="PK218_03415"
     CDS             complement(<1..932)
                     /gene="hemE"
                     /locus_tag="PK218_03415"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HPW97589.1"
                     /translation="MIKNDLFLKALNNETVNRPPVWMMRQAGRYLPEFMAIKEKYDFF
                     TRCKTPELASEITVQPIRIIKPDAAILFSDILVVPQAMGIQVELQDNIGPVLPNPIRK
                     ASDVDNVFIPDINETLGYVMDAIDMTREKLNNEVPLIGFAGSPWTIFCYAVEGKGSKS
                     FDKAKGFCFTEPAAAHALLQKITDTTILYLKEKVKHGVNAVQIFDSWGGMLSPTDYQE
                     FSWFYMNQIVEALAPETKVIVFGKGCWFALNDMAKSKASALGVDWTITARNARYLTGG
                     KITLQGNFDPSRLLSPIPTIKKMVHQMIDEFGKD"
     gene            complement(1008..1682)
                     /locus_tag="PK218_03420"
     CDS             complement(1008..1682)
                     /locus_tag="PK218_03420"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPW97590.1"
                     /translation="MTKQINILSTKKLSEIQKKALLDANFEVIDEDFININYKKGLSF
                     GEDLGEALIFTSQNAVQSVLNHPKWEELKTKNVFCVGIKTKELLTENGFNVVAYTGYA
                     ADLAEIITLIYNKESYTFFSGNLRRDILPNTLKENGIQFNEIEVYETNLTSKKITQKL
                     NGILFFSPSGVESFLEKNKITNEICFCIGETTAEALENKNIKNIVIAQKPSVENVITE
                     VVEYYK"
     gene            complement(1682..2308)
                     /locus_tag="PK218_03425"
     CDS             complement(1682..2308)
                     /locus_tag="PK218_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97591.1"
                     /translation="MNAKSVKDELKNILQGTSNNCQETLVQTIACYLRASQRTSTLVE
                     NDKQIREKETKSLIEYCNQNHLWYNDKIDVNLFISEGAEQKVYIKDNKTVYKLNDAIY
                     YTKWLDYFNSLLLNNYFFPDTSYTLVGFCLIDEILYAVVEQPFVKATHPTDLNKVKEF
                     MAANGFKNTRNNDYYNADLGIILEDLHDENVLVQEDVLYFIDTVFYIK"
     gene            complement(2348..3316)
                     /gene="hemC"
                     /locus_tag="PK218_03430"
     CDS             complement(2348..3316)
                     /gene="hemC"
                     /locus_tag="PK218_03430"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPW97592.1"
                     /translation="MAKSTPNKTIRIGTRDSELALWQAHTVQKQLQDLGYKTQIVAVK
                     SDGDIQLDKPLYELGITGIFTKTLDIAMITGKVDIAVHSMKDVPTALPQGIVQAAVLE
                     RANTLDILVHKGNLDFLENSGTIATGSLRRQAMWLNKYPNHKVVDLRGNVNTRMQKLQ
                     ENDWNGAIFAAAGLERLGFINENAIKSPSFGGVGEALDWMIPAPAQGAMVVVAMAEDF
                     YTIEALSQLNHIETEICTYIERQFLKTLEGGCTAPIGALANYNEKEDVLEFSGVLLSI
                     DGKQKLEITKKAEVSEWKKLGYHCAQEILNNGGSQLMSEIKAQLKK"
     gene            complement(3316..4566)
                     /gene="hemA"
                     /locus_tag="PK218_03435"
     CDS             complement(3316..4566)
                     /gene="hemA"
                     /locus_tag="PK218_03435"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPW97593.1"
                     /translation="MDNKHLSKPTTFYAIGLSYKKADADIRGKFSLDTNAKQALLDQA
                     TLAGIESLIVTSTCNRTEIYGFAEHPFQLIKLLCDNSSGTVEDFQKVAYVYKNQEAIS
                     HLFKVGTGLDSQILGDFEIISQLKTAFTESKSKNLMNAFLERLINSVIQASKKIKNET
                     EISSGATSVSFAAVRYIFNNVENISNKNILLFGAGKIGRNTCENLVKHTKHEQITLIN
                     RTKDKAERLARKLNVIVKDYTDLQLEIQKADVLVVATGAQNPTVDKAILNLKKPLLIL
                     DLSIPKNVHENVQDLNNVALVHMDHLSQMTDETLENRKKHIPAAEAIIEEIKEEFITW
                     TKGRKFAPAIHALKAKLNTIKEKELNSQRKKISNFDEEQAEMISNRIIQKITNHFANH
                     LKDENTMVDESIEWIEKIFQIEAK"
     gene            4797..5669
                     /locus_tag="PK218_03440"
     CDS             4797..5669
                     /locus_tag="PK218_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW97594.1"
                     /translation="MGSKEEIKIDDDFILIRFQNDTEEVSTFQRSVPLGLIQFHFGLK
                     GSADYIFNQGNYILNLKEEKALLFYNPEKELPLNVEIAPKSWLISILVSIKKFHGLFT
                     TDAEHIPFLSQENKDKKYYNESDVSPSMAIVLNQMFHYNLNPSIKNLYYKGKGYELLS
                     LFFNRNEDPNAEQCPFLVDEENVLKIKKAKEIIIANMAEPPSLQELADQVGLTLKKLK
                     MGFKQIYGDTVYAFLFDYKMEHARQLLDTGSFNVNEVGLKIGYSTGSHFIAAFKKKFG
                     TTPKKYLMSLNISS"
     gene            5741..6757
                     /gene="hemH"
                     /locus_tag="PK218_03445"
     CDS             5741..6757
                     /gene="hemH"
                     /locus_tag="PK218_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HPW97595.1"
                     /translation="MKGVLLVNLGSPESPTPKDVKPYLDEFLMDKYVIDVPYLLRALL
                     VRGIILQTRPKNSAHAYAQIWTDEGSPLIVFSKKMHEKVAKQVDIPVALAMRYGTMTI
                     EKGLQELKEKGVTDVMLLALYPQYAMASTTTIWALADELVAKKFPGMKLTKVPAFYNK
                     PDFIKTLANSIKKHLEGFDYDHLLFSYHGIPKRHIRKTDVTKSHCTIDAKCCVTASPA
                     HEFCYRHQCFETTRQVVELLGIPEDKYSQTFQSRLAGDKWLTPYTDVEINKMPVKGIK
                     KLAVVTPAFVADCLETLEEIAMRANEDFKENGGEEFFAVPCLNDEDEWCDVVANWIED
                     FKKN"
     gene            6822..7376
                     /locus_tag="PK218_03450"
     CDS             6822..7376
                     /locus_tag="PK218_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HPW97596.1"
                     /translation="MEFYNYLKSLHLIFVITWFAGLFYIVRLFVYQIEANDKPSPEKE
                     ILQKQYKIMTYRLWYIITWPSAILASFFAFWMLFFTDLGSAWLKMPWMHVKLGFVFVL
                     YLYHLKCHQIYKQLQNDDFKYSSNFMRLWNEGATLILFAVVFLVILKNAINWIYGVIG
                     IILFSVLIMLGFKFYKRIREKNKS"
     gene            7437..8849
                     /locus_tag="PK218_03455"
     CDS             7437..8849
                     /locus_tag="PK218_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW97597.1"
                     /translation="MIFLILMASILMASISIIQFKNEAKDYHQERLDRKEFAIKEHIN
                     YVLSTTTYPLTPENLPLIFKDKIHELADIHNLEINIYSLDGKLLKSSKAKFSVDKVSP
                     PIDKFILKLVQSSVEKRYVDIKNINGVKNRSSYSQIKDEKFKPLGILNIPYVEDDGFY
                     EEELKQFLLRLSQVYSFMLVIAFALAYFLASYITKSLKTISDKINETSLNQKNEKIVI
                     EANSREINSLIAAYNGMVDKLEESASKLAQSEREQAWREMAKQVAHEIKNPLTPMRLT
                     VQSFQRKFDANDPELKQKLDDYSKTLIQQIDTMSAVASAFSNFATMPAQQNESLNVVD
                     VVELSLDIFNEDYIVFESNKEEIITKLDRTQLIRIITNLVKNAIQSIPEEQKEKSVLV
                     SVNEVDHNVIISVKDNGIGIELYNQSHIFEPKFTTKTSGMGLGLGIIRNIIENYKGTI
                     TFETEFGKGTTFIVSLPIIN"
     gene            8861..9643
                     /locus_tag="PK218_03460"
     CDS             8861..9643
                     /locus_tag="PK218_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPW97598.1"
                     /translation="MTYENILTSTENGIGQITINRPSKLNALNVATIQELHDAFDTLD
                     KNSEVKVIIITGEGEKAFVAGADISEFASFSVEEGAQLAAQGQELLFDFVENLKTPVI
                     AAVNGFALGGGLELAMSCHFRVASDNAKMGLPEVSLGVIPGYGGTQRLPQLVGKGRAM
                     ELIMTAGMIDAETAKSYGLVNHVVPQAELLEFTKNIASKIMRNSPMAIGKAISAVNAN
                     YKDGVNGFETEIKNFGKCFGTEDFREGTTAFLEKRKASFSGK"
     gene            9707..11377
                     /locus_tag="PK218_03465"
     CDS             9707..11377
                     /locus_tag="PK218_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97599.1"
                     /translation="MFSQNINYKKVKSDIFQDDYKNSVIVLSEENTNGELLLVRSYKG
                     TIVSQGEGFYIEYYDTNLKFKRAFEYSMNHPNYQKYNLILGVFMMQNNIHFVEIYYDL
                     NEKSFVCLDNILSQDFSSTKKELFRISRDEMKNYGKFNLQQKFYDRTKEIWTNDNSGK
                     INSENELANLSGFHKVFFSGNNNYKKIIENENQGNGSDIILVVNEQKNSFLVALDFNQ
                     EEKDGLKLYLYDDKGNKKFETIYSNTIEDKKYFFQNLQISEDGNFIYLLAKTYSFDAK
                     KKNEGGKYFYEFTKISNYNQLTTTLNTNEHFVGSLNTFIHNDELTCLGFYSDVKDFKY
                     SGICCFKIDPNTLEVKSANYNPFTEQFLFDKYGDSKPKDLKNLNIKKVFFTENNEILL
                     NAEENYISQSGVVGYSGANNSPMQFGNSDKYYCYDDIVIGKLDALGTLQWARNINKKQ
                     STNDDDNSFISYTSTIRNNKNYLFVNTKDKIKKLKNDRIEFGQIRKNKSNLNVIQVDE
                     NGDFEYEEILDDEQNEVPFMVSKGAIIDNSVYFLGRKGRNKQLLKVTL"
     gene            11379..11687
                     /locus_tag="PK218_03470"
     CDS             11379..11687
                     /locus_tag="PK218_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97600.1"
                     /translation="MVKTIFLLFLTSFCFSQKQITFIDSLTKRPIGYASIWKENQIYK
                     TTDSLGVLNIENDLLDKTFKITALGYKEKVFKIVTNEVSLSPETSLLNEVKIYKRKKP
                     "
     gene            complement(12207..12851)
                     /locus_tag="PK218_03475"
     CDS             complement(12207..12851)
                     /locus_tag="PK218_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW97601.1"
                     /translation="MSLIDSTISFVKDQLKTAEGGHDWFHIERVYRNALLIAESETCN
                     IEIVKLGALLHDIADSKFHNGDETIGPKTARIFLESQNVSEEIITHVINIIQNISFKG
                     GNFDKKFNSIELEIVQDADRLDAIGAIGIARTFNYGGFKNRQIYNPAIAPKLTMSKEE
                     YKNSDAPTLNHFYEKLLLLKDKMNTETGKKIALERHKFMENFLSQFYAEWEGEK"
     gene            complement(12882..13868)
                     /locus_tag="PK218_03480"
     CDS             complement(12882..13868)
                     /locus_tag="PK218_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HPW97602.1"
                     /translation="MSQKNMRLEVMQFLENKVDSFVDQFLIPVEKIWQPSDFLPNSEG
                     ETFFEEVKELREIAKDLPYDFWVVMVGDTITEEALPTYESWLMEVEGIDNEGRNGWSK
                     WVRQWTGEENRHGDLLNKYLYLSGRVNMREVEITSQHLINDGFDIGTGKDPYKNFVYT
                     SFQELATYISHNRVSQLAKQYGDKQLSKMCKMIAGDEMRHHHAYSEFVTQIFKVDPSE
                     MMLAFQYMMKQKITMPAHFLRESGEKIGTAFEEFSNSAQKIGVYTASDYVDILKKLTE
                     KWEIDKITNLTAEAEKARDYLMKLPERMAKISERLVLPAETKIFKWVEPAIV"
     gene            complement(13910..14641)
                     /locus_tag="PK218_03485"
     CDS             complement(13910..14641)
                     /locus_tag="PK218_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW97603.1"
                     /translation="MEKLISYPISVVYYLCFGLCLVIFHPIQWFCLNVFGYQAHKKSV
                     DYLNFFLVKCTNILGTTYTFKNRNTIPKNAPIIFVSNHQSMYDIVGIIWYLRKFHPKF
                     VSKIELGKGIPSVSYNLRHGGSVLIDRKDPKQAIPVIKSLGEYIEKYNRSAVIFPEGT
                     RSKTGKPKEFAQSGLKILCKSSPSAFVVPISINNSWKMVKFGAFPMGLGNRLEFIIHQ
                     AIPVKDFPFEELIKKTEQSVVSSIK"
     gene            14775..15779
                     /gene="recA"
                     /locus_tag="PK218_03490"
     CDS             14775..15779
                     /gene="recA"
                     /locus_tag="PK218_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPW97604.1"
                     /translation="MSSEKEAKLKALQLTLDKLDKAYGKGTVMKMGDKAVEEVETISS
                     GSLGLDLALGVNGYPKGRVIEIYGPESSGKTTLTLHAIAEAQKAGGIAAFIDAEHAFD
                     RNYAEKLGVDIENLIISQPDNGEQALEIAENLIRSGAIDIVVIDSVAALTPKSEIEGE
                     MGDSKMGLHARLMSQALRKLTGTISKTNCTVFFINQLREKIGVMFGNPETTTGGNALK
                     FYASVRLDIRRSSQIKDGENVIGNRTKVKVVKNKVAPPFKTAEFDIMYGEGVSKTGEI
                     LDLAVEFEIVKKAGSWFSYGDTKLGQGRDSVKVLIKDNPELADELEQKIKEYIKETNS
                     "
     gene            15882..16289
                     /locus_tag="PK218_03495"
     CDS             15882..16289
                     /locus_tag="PK218_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97605.1"
                     /translation="MKRVLFALLLSLTLLSCSLDDGDKLYLEIVPVSAVEMPTAFRVD
                     SITQIPISYIRPTPCHEFSNFYYNSIGNERTVAVYCLTNGNQNCVPSTDYEITVPLNF
                     KPKEMGTYHFRFWIGENENGVDQYIEHEITVDH"
     gene            16301..16849
                     /locus_tag="PK218_03500"
     CDS             16301..16849
                     /locus_tag="PK218_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW97606.1"
                     /translation="MSIEELIHGCKKKDIKAQEQLYRQYAPKLFSVCLKYSRNYSEAQ
                     DNLQDGFLLIFEKINQFAFKGAFEGWIKRVMINFILQQYRKETFLNLVHENFPKEIEI
                     EIENESEIPIEFLMKIIQELPDRYRLVFNLYVIDGYSHQEIADMLSINIGTSKSNLSR
                     AKVILKEKIETITGSKNIPSIK"
     gene            16846..18402
                     /locus_tag="PK218_03505"
     CDS             16846..18402
                     /locus_tag="PK218_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97607.1"
                     /translation="MKENKNIDQFFKDKFENFEINPPEEIWKNIEEKLQKKKKRRVIP
                     FWWFGSGVAALFVIGLFIFNNNYTVEIHPVNDVVIENNTPKKTNSIQENNNENQGIIK
                     ENTEIVTSESDKNKASEKINTETNKAYKESLNSTENSSIVSSEKKSTIIYNDYKNSIK
                     KEKNTIQNSNNTIVNNTKSSAKKNNKISNKSNVDIKSKITEIENGTVEKQENIVQNNS
                     RFNETKSELNKENNIELGKIAMKNHNNVATKGEVNTSKDSTAIATVETNELEELLKEK
                     ENQITKEQKMNRWQVTPNLAPIYFGSFADGSPLDSSLDENSKSFNNNLSYGVAVNYSI
                     NKKIKIRTGINSFSVDYNTNDIIFFQSTNARMMKNVTPTVEGSLIEIIPIKNLNAAFN
                     RLETEQNDGVLNQRMGYIEMPMEISYKLLDNKFGVELIGGMSALFLNKNEVYLEADGI
                     TLKVGEASNLNPMHFSGNFGVGFNYGFFKKFQARIEPTFKYQLNTFSRDSQDFKPYIF
                     GIYTGISYNF"
     gene            complement(18491..19225)
                     /locus_tag="PK218_03510"
     CDS             complement(18491..19225)
                     /locus_tag="PK218_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW97608.1"
                     /translation="MKVLKYIFWILYRIWFYIMMAIPILIMFPFLFLSILKETWYPYF
                     FMMARIWAKCILIGMGFNTKVVFEQHPEKGRSYMFVANHTSMIDIMLMLSIVENPFVF
                     VGKKELAKIPLFGFFYKRTCILVDRSSPKSRMEVFERAQKRLKQGMSICIFPEGGVPD
                     ESIVLDEFKDGAFRLALEHEIPVVPMTFYDNKKRFSYTFFSGSPGQLRAKVHHFIETK
                     GKSEIKKTLKTEVRTLILNELQNDSK"
     gene            19298..20275
                     /gene="trpS"
                     /locus_tag="PK218_03515"
     CDS             19298..20275
                     /gene="trpS"
                     /locus_tag="PK218_03515"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPW97609.1"
                     /translation="MSKILTGIQSTGTPHLGNLLGAILPAIELSKNPNNESFLFIADL
                     HSITQIKNGDELRQNTYSTASVWLACGLNVDKVVFYRQSDVPQTAELSWYLSCFFPFQ
                     RLTLAHSFKDKADRLEDVNAGLFSYPMLMAADILLYDANVVPVGKDQMQHIEITRDVA
                     SRFNHQMGETFVLPEGKVQEETMYIPGTDGQKMSKSRGNLINIFLDDKALRKQIMSIE
                     TDSTPLEEPKNPDTCKVFAIYKLLANSEQIASIKKKYTNESRDFGYGHAKQALFELIV
                     EKFKTEREKYNYYMNNLEEVDSLLKLGAAKAGSIADGVLNRVREKLGFN"
     gene            complement(20272..21705)
                     /locus_tag="PK218_03520"
     CDS             complement(20272..21705)
                     /locus_tag="PK218_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97610.1"
                     /translation="MNIIQLFETLAIDKSRIGALSQDDFSTIENVLLIEKEKNQLISD
                     ISITNFLNALKFHSESFRGIFENRVLYNFFTNKDQPRDFFKPETVSNDVDKMKTFVGL
                     FFGSEIKAFFNQSFEENQFYKASILAEAFPYFPDTIAYYVLFEAKDCLDIAHSKLIPP
                     YSDLSKIEYLKDSNFYAFLSKIKTPKLEEKLHSVFELLINMFNQNSYSEMAGKAFLAT
                     GSYVALDGNFAEKIRVYREKGSEFYKPFAPKKKNRTWIYVVVGTFVLLRIGFFISSSN
                     LFNKSNNYSNDDYTIDETYNDTKPTLDPYYSEMKFKIDSFHTFLSEFNASEIKNFTIN
                     SALKSGENPFETFYKNVEKSESSDFIKVKNTSVYDMVLLENNVIYDSIKMPVTAYYLK
                     AGENAILNSVASNAVFNIYIGKNLASFNTNSNHLFIRNQSIVEYRFKELAPQTKDLLK
                     ADFYFDDDVTISFKNGDIKIDSRVIMD"
     gene            complement(21754..22857)
                     /gene="dprA"
                     /locus_tag="PK218_03525"
     CDS             complement(21754..22857)
                     /gene="dprA"
                     /locus_tag="PK218_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPW97611.1"
                     /translation="MIPNELFHVLALLKVDGVGDIVAKKLINHCGSAEEVFKSKRQQL
                     QSIDGIGDMLLKKLKDKSVFEKAAKELQFIEEENFNVLYYQETAYPEKLKHCIDGPTL
                     LFSSGNFDLNTRKIISIVGTREVTSYGTEFCKKLIEDLTIFNPIIVSGFAYGVDIVAH
                     KAAMDNNLQTIGVLAHGLNQIYPKVHKKYVAKMEENGGFLTEFWSNSNPDKENFVRRN
                     RIVAGMSEATIVIESAEKGGSLITANLANDYNRDVFAVPGRISDKFSQGCNDLIKTQR
                     ATLMTSAADLIYNLSWELESKPTKSVQKQLFVSLDNDEQLIYDYLQKNGKQLLDSIAI
                     ECSFPIFRISSILLNMELKGVIRPLPGKLFEAI"
     gene            22960..23904
                     /locus_tag="PK218_03530"
     CDS             22960..23904
                     /locus_tag="PK218_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPW97612.1"
                     /translation="MKIEQYISQLLYRYQCVTVPGFGAFLTEIQSAKLDENTNSFYPP
                     KKLVSFNPYIKNNDGLLANHLSKEEKINYEVAISVIQTEVENWRKSIHEFGQLTFKNI
                     GGFSLNSENNLVFVPAEQVNYLKESFGLSTFVSPTVKREEYKEIVEELEEKAPIVFTP
                     EKRRNYSFLKYAAIVLLSVGVSGAIGYKVYQNYEQEIAQQTLLVEQNVQKKVNQKIQE
                     ATFFIESPLPSVTLTIPSEKMPYHVVAGAFSIESNADNLVERLLKLGYNAKRIAPNKF
                     GLNPVLYGSYSSYSEAYKAMKEIQQADNPKAWLLIEEF"
     gene            23967..24476
                     /locus_tag="PK218_03535"
     CDS             23967..24476
                     /locus_tag="PK218_03535"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPW97613.1"
                     /translation="MHPKPASESLTILTDLVLPSETNPLNNLFGGELLARMDRAASIA
                     ARRHSRRITVTASVNHVAFNRSIPLGSVVTVEAKVSRTFRTSMEIFLDVWIEDRESGT
                     KTKANEAIYTFVAVDETGRPVEVAPVIPETELEKQRFDAALRRKQLSLVLAGKMKPSE
                     ATELKALFI"
     gene            24528..26441
                     /locus_tag="PK218_03540"
     CDS             24528..26441
                     /locus_tag="PK218_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPW97614.1"
                     /translation="MKITFFSLAFLLFFAIDGNAQKGLDPTADEISQAKKLRESYSKD
                     DIAILESSELITFDLNKKAAKVIVNHSIKEHLMNINHRADIQKYEFYDSESSIQNFSL
                     KYRNDKTASFSVKDEFYKDRDLFYNDAKVKYVTIDFPVQGYNYKYEMEKKYDDVKYFT
                     SLFFNDEYPVLSKKITFEIPNWLDVELKEFNFDGFKINKTSNTEDKKTIVTYTLENVP
                     AAFKENSMPGRSYLYPHILVIAKSFTKDGATTTLFKSNADLYNWYKSLIAMMKDDTTV
                     MKSKVNELTANAKTDEEKIKNIYYWVQDNIRYIAFEDGIAGFKPDESNNVFEKRYGDC
                     KGMANLIKQMLKLAGFDARLTWIGTKHIAYDYSTPSLAVDNHMICTLMYKGKKIFLDG
                     TEKYNSFGEYAERIQNKEAMIEDGDKCIIEKIPTTTADFNKETFKTTLAIENETLIGV
                     CNKTYQGESRAEFLNIYNGFENTKRKERLDGYLSKQNKSIAVSDVKTSDLQNRDSKLT
                     IDYNIAIQNKISSFDDEIYVDLEFMNEYKNFELKDRKVAYEMGYKTNYVSQINLKIPD
                     GYNVTKMPTNLSIKENDFDINLSYENTGKEIIYKKSFMFKNGLIKADAIEKWNEFNKK
                     LKENYNQQITLSK"
     gene            26457..28610
                     /locus_tag="PK218_03545"
     CDS             26457..28610
                     /locus_tag="PK218_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPW97615.1"
                     /translation="MKTKLALLFFLAIIGICSAQDAKEAREFFWGAKDEFKKANTIPD
                     KWKNESAVIIHKREHYNYHKFGKSVTYVSGIRKRIMLLDQAAVTEFSQFSYKNKFYSN
                     RAYGYKKGTNYVGVKIIKPSGKEIEIDTDKESKEVDGEKKLAVPNLEIGDIIDYYFYS
                     VEPFKSFFDYTFEAVETPLGDVYPTMDLKITLDTENDFFINFNTYNGAPNLKEIPTKN
                     SGERSYELSKKDIPKNEFPRWFYPLAEMPCYKFQVIFARSGKFEKLADGFLSEKERDI
                     KKTVSKEDIFDYYNKKFVPAGDIGDINDFLKGKTFTSEEDKIREVYYFTRHQFYTRYI
                     EAFVVNEAKIFYPFEYYGNYPIFFNSEVQFVNYFMQFLRKNDISYDVIIATPRYDGSI
                     DDLLIQSNVKKLIRVNTKNPVYLEYFSPYTSADQFNYNIENSKAYVLEVSKGKRITDA
                     STVQLPSSTYQENSVKTKTELTLEDFTGFKAKKLSSYLGHLKDSEQSDRLNFYDYVYE
                     DYKKYATKPLLDYVKNKKKNEQYSNEMKALINKMKDTQKESFEKSIEGELDFLVDDYS
                     FKINNTGRYGKKSSFEMEEEFSIKDNLIKKAGDKYLIELGKLLTSQIEIDQKEKDRKN
                     NIYSVYPRSYENEIILNIPVGYSVSGLEKFNKKVENETGGFTSTAEVKGNQLIVKTYK
                     HYKNYFEPNANWNKMIDFLDAAYQFTQEKVLLKKN"
BASE COUNT        10078 a         4426 c         4619 g         9510 t
ORIGIN      
        1 ttgtcttttc cgaattcgtc aatcatttga tgcaccattt tcttgatggt tggaattggc
       61 gaaagcaaac gacttgggtc aaaattacct tgcaacgtta tttttccacc tgttaggtaa
      121 cgtgcgtttc tggcggtgat tgtccaatcg acaccaagcg ctgatgcttt tgattttgcc
      181 atatcgttta gggcgaacca acatcctttt ccgaagacga taactttggt ttctggagca
      241 agagcttcga cgatttggtt catatagaac catgaaaatt cctgataatc ggttggtgac
      301 aacattccgc cccaagagtc gaaaatttga acggcgttga caccgtgttt tactttttct
      361 tttaaatata aaatggtagt atcggtgatt ttttgcaaca atgcgtgtgc tgctgcgggt
      421 tctgtaaagc aaaaaccttt ggctttatcg aaacttttgg aaccttttcc ttctacggca
      481 taacagaaaa tcgtccaagg tgaacctgca aaaccgatta atggtacttc gttgtttaac
      541 ttttcacgtg tcatatcgat ggcatccatc acgtagccca atgtttcgtt gatgtctgga
      601 atgaacacat tatcaacatc agaagctttt cggattgggt ttggtaaaac tggtccgata
      661 ttgtcttgca attcgacttg aattcccata gcttgtggca caactagaat gtctgaaaac
      721 agaattgccg catcgggttt tataatgcgt attggttgta ccgtgatttc ggatgctagt
      781 tctggtgttt tgcaacgggt gaagaaatcg tatttttctt tgattgccat gaattctggc
      841 aaatatctac cggcttgacg catcatccaa acgggtggac gatttacggt ttcgttattt
      901 agggctttta ggaataggtc gtttttaatc atttttattt cttctttacc acaaattaca
      961 caaattttca caaagagatt tttgaaaatt aataacttgt ttaagtttta cttataatat
     1021 tctactactt ctgtaatcac gttttctact gatggttttt gtgcgattac aatgtttttg
     1081 atgtttttat tttctaatgc ttctgcggtg gtttcgccaa tacagaaaca aatttcgttt
     1141 gtgattttat ttttttctaa aaaactttca acgccagatg gactgaaaaa aagtattcca
     1201 tttaactttt gagtaatttt tttagaggtt aaattggttt catagacttc aatttcgttg
     1261 aattgaattc cgttttcttt taacgtgttt ggtaaaatat ctctgcgcaa atttccactg
     1321 aaaaaagtgt agctttcctt attgtatatt agtgtgatga tttcggccaa atcggctgcg
     1381 taacctgtat atgcaacgac attaaaccca ttttcggtta ataactcttt ggtttttatt
     1441 ccgacacaga acacgttttt ggtttttaac tcttcccatt ttggatgatt taaaacactt
     1501 tgcacggcgt tttgactggt gaaaattaaa gcctctccta aatcctctcc aaaggagagg
     1561 ccttttttat aatttatatt tatgaaatct tcatcaatta cttcaaaatt ggcatctaac
     1621 aaagcttttt tctggatttc agaaagtttt tttgtggata gtatgttgat ttgttttgtc
     1681 attactttat ataaaacacg gtatcgatga aatataaaac atcttcttgc accagcacat
     1741 tttcatcatg taaatcttca agaattattc ctaaatcagc attataataa tcgttatttc
     1801 gagtattttt aaaaccatta gcagccataa actcttttac tttatttaaa tctgtaggat
     1861 gagttgcttt tacaaaaggt tgttcaacta ctgcgtaaag tatttcatct atgaggcaaa
     1921 aacccacgag tgtataggaa gtgtcaggga agaaataatt gttaagcaat aaactattaa
     1981 aataatccaa ccatttagta taataaatgg catcatttag tttgtaaaca gttttgttgt
     2041 cttttatgta aactttttgt tcggcacctt cagaaataaa caaattgacg tcaattttat
     2101 cattatacca aaggtgattt tgattgcagt attcgattaa actctttgtt tctttttctc
     2161 tgatttgctt atcgttttca accaaagtac ttgttctttg gcttgctcta aggtaacagg
     2221 cgattgtttg gacaagcgtt tcttggcaat tattgctcgt tccttgaagg atatttttaa
     2281 gttcatcttt taccgatttt gcgttcatac aaatgtaaca taattttttt aagcctttat
     2341 tatcaggcta tttcttcaat tgagctttaa tttcactcat caactggctt ccaccattat
     2401 ttaaaatctc ctgagcgcaa tggtaaccca gttttttcca ttcggaaact tctgcttttt
     2461 tagtaatttc caatttttgc tttccatcaa tagaaagtaa aacacctgaa aactccaaaa
     2521 catcttcttt ctcattataa ttggctagag ctccgattgg cgccgtacat ccgccttcga
     2581 gcgttttgag aaattgtctt tcgatatagg tgcaaatctc ggtttctatg tgatttaatt
     2641 gagacaatgc ttcgatggta tagaaatctt ctgccattgc tacgactacc atggcacctt
     2701 gagctggtgc tggaatcatc caatcgagtg cttccccaac tcctccaaag gaggggcttt
     2761 ttattgcgtt ttcatttatg aaccccagtc gttccaaacc tgcagctgca aaaatggctc
     2821 cgttccaatc attttcttgg agtttttgca tgcgtgtatt aacgtttcca cgtaaatcga
     2881 ccactttgtg atttggatat ttattgagcc acattgcctg acgacgcaga cttcctgtgg
     2941 caattgttcc tgaattttct aaaaaatcaa ggtttccttt gtgaactaaa atgtctaacg
     3001 tgtttgctct ttctaaaacc gcagcttgca caattccttg cggcaaagct gttggcacgt
     3061 ctttcatgga atgcacagca atatcaactt taccagtaat catggcaatg tctaatgttt
     3121 tggtaaaaat cccagtaatt ccaagttcgt aaagtggttt gtctaattga atatcgccat
     3181 ctgattttac cgcaacaatt tgtgttttgt accctaagtc ttgaagttgt ttttgaacgg
     3241 tatgtgcttg ccaaagtgct aattcgctat cacgagttcc gattctaatg gttttatttg
     3301 gagttgattt tgccactatt ttgcttctat ttgaaatatt ttttcaatcc attcgatgct
     3361 ttcgtccacc atcgtgtttt catctttcag atgattggcg aagtgattgg tgattttttg
     3421 gatgattctg ttgctgatca tttcggcttg ctcttcgtcg aaattagata tttttttgcg
     3481 ttgtgaattg agttcttttt ctttaatcgt attcaattta gcttttaaag catgaatggc
     3541 tggagcaaat tttctgcctt ttgtccaggt gatgaactct tcttttattt cttcgataat
     3601 ggcttcggcg gcaggaatgt gtttttttct attttctaaa gtttcatcgg tcatttgaga
     3661 taaatgatcc atgtgtacca atgcaacatt gtttagatct tgaacatttt catgtacatt
     3721 ttttggaata gacaaatcta aaatcaacaa tggtttcttt aagttcaaaa tagctttgtc
     3781 aaccgttggg ttttgtgctc cggtggcaac cacaagaaca tctgcttttt gaatttcgag
     3841 ttgtaaatcg gtgtaatctt taacaattac atttagtttt cttgccaatc gttctgcttt
     3901 gtctttggtt cgattgataa gagtaatttg ttcgtgtttg gtatgtttta caagattttc
     3961 acaggtattt ctacctattt ttcctgcacc aaacaacaaa atatttttat tggaaatgtt
     4021 ttctacatta ttgaaaatat agcggacggc agcaaaggaa acagatgttg cgcccgaaga
     4081 aatttcggtt tcgtttttta tttttttact ggcttgaata actgaattga ttaatcgctc
     4141 cagaaaagca ttcattaaat tcttggattt actttcggta aaagcagttt ttaactggct
     4201 gataatttca aaatctccta aaatttgact gtctaaacca gttccaactt tgaataaatg
     4261 acttatcgct tcttgatttt tatagacgta agctactttt tgaaaatctt caacggttcc
     4321 agaactatta tcgcatagta attttatcaa ttgaaaagga tgttcggcaa aaccgtaaat
     4381 ttcagtacga ttacaagtag aagtaacaat taaactttca attcctgcca atgttgcttg
     4441 gtcaagaagt gcctgttttg cattggtatc caaactaaat ttacctctaa tatcagcatc
     4501 ggctttttta taactcaaac caatggcgta gaaagtagta ggtttagaaa ggtgtttgtt
     4561 atccataaat gcaactttat caaaaagctg aagcaaaatt atagttatac tttttataaa
     4621 aacaacgctt aaagaacttt ttgtgtcgct attggatatt tttctaagga tttgtctttt
     4681 tagcgatatt tattctaaat ttgtagaaaa ataaggtgtt tacaatgtct ttatgtagaa
     4741 taattctaaa taacattaat tgtaatatat cgaaatattt ctaaaaaaat atcgctatgg
     4801 gttcaaaaga agaaattaag atagacgatg attttatatt aattcgtttt caaaacgaca
     4861 ctgaagaagt ttccactttt cagcgttcag ttccattagg gttaattcaa tttcactttg
     4921 gcttaaaagg aagtgccgac tatatcttta atcaagggaa ttatattttg aacttgaaag
     4981 aagaaaaagc gttgttgttc tacaatcctg aaaaagagtt gcctttgaat gttgaaattg
     5041 ctccaaaatc atggttgatt tcgatattgg tttctatcaa aaaatttcat ggattattca
     5101 ctaccgatgc cgaacacatt ccatttttaa gtcaagaaaa caaggataag aaatactata
     5161 acgaaagtga tgttagccca tcaatggcaa ttgtgctcaa tcaaatgttt cactacaact
     5221 taaatccatc gataaaaaat ttatattaca aaggaaaagg ttatgaatta ttaagtcttt
     5281 tttttaatcg aaatgaagat ccaaatgcag aacaatgtcc gtttttagtc gatgaagaga
     5341 atgttttaaa aataaaaaaa gccaaagaaa ttatcatcgc taatatggca gaacctccaa
     5401 gtttacaaga acttgccgac caagttggtt tgacgttgaa gaagttaaaa atgggtttca
     5461 agcaaattta tggcgatacc gtttatgctt ttttatttga ctataaaatg gaacatgcgc
     5521 gccaattgct cgatactggt tcgtttaatg taaatgaagt tggattaaaa attgggtaca
     5581 gtaccggaag tcattttatt gctgcattca aaaagaaatt tggaacaaca ccaaaaaagt
     5641 atttgatgag tttaaatatt tcatcataaa caataagttt ttaaaataaa atcataaaaa
     5701 aataagtttc cctactttta taaaaaatta aatagccaaa atgaaaggtg tattattagt
     5761 aaatctgggt tcgccagaaa gtccaactcc aaaagatgta aagccgtatt tagatgaatt
     5821 tttaatggat aaatacgtaa ttgatgttcc gtatttgttg cgtgcattgt tggttcgtgg
     5881 aattattttg caaacacgtc caaaaaattc cgctcatgct tatgcgcaaa tttggaccga
     5941 tgaaggttcg cctttgattg ttttttctaa aaaaatgcat gaaaaagtag ccaaacaagt
     6001 tgatattccc gtagcattag ccatgcgtta tggaaccatg actattgaaa aaggtttgca
     6061 agaattaaaa gaaaaaggtg taactgatgt aatgcttttg gcattatatc cgcaatatgc
     6121 tatggcttca actactacta tttgggcttt ggcagacgaa ttggttgcga aaaaattccc
     6181 tggaatgaaa ctgaccaaag ttccagcttt ttataataaa cccgatttta tcaaaacatt
     6241 ggcaaattct atcaaaaaac atctagaagg ttttgactac gatcatttgt tgttttccta
     6301 tcacggaatt ccaaaacgtc atattcgcaa aacggatgta accaaatcac attgtaccat
     6361 tgacgcaaaa tgttgtgtta cggcttcacc tgctcatgaa ttttgttatc gtcatcaatg
     6421 ttttgaaacg actcgacaag ttgttgaatt attaggaatt cccgaagata aatacagtca
     6481 aacattccaa tctcgtttag caggtgacaa atggctaact ccttataccg atgttgaaat
     6541 aaataaaatg cctgtcaaag gaatcaagaa attagcggtt gttactcctg catttgttgc
     6601 cgattgttta gaaactttag aagaaattgc catgcgtgcc aatgaagatt ttaaagaaaa
     6661 tggcggtgaa gagttctttg ctgttccttg tttgaacgat gaagatgaat ggtgtgatgt
     6721 tgttgcaaat tggattgaag attttaaaaa aaactaattt agtttacagt ctcagccacg
     6781 taaacagatg cgactgaaaa ctgaaaattg aaaattgaaa tatggaattc tacaactact
     6841 taaaatcact tcatttaatc tttgttatca cttggtttgc tgggttgttt tacattgttc
     6901 gcttgtttgt ctatcaaatt gaagctaatg ataaaccttc acctgaaaaa gaaattttac
     6961 aaaaacaata caaaatcatg acgtatcgtt tgtggtacat tatcacttgg ccaagtgcta
     7021 ttttggctag tttttttgcg ttttggatgt tgttttttac cgatttagga agtgcttggc
     7081 taaaaatgcc ttggatgcat gtgaaacttg gcttcgtttt tgtgctttat ctgtatcatt
     7141 tgaaatgtca tcaaatttat aaacaattgc aaaacgatga ttttaaatac agttccaact
     7201 tcatgcgttt gtggaacgaa ggcgcaacat taatattgtt tgccgttgtt tttttagtca
     7261 ttttaaaaaa tgcaatcaac tggatttacg gtgtaatcgg aattatactt ttttctgttt
     7321 tgattatgct cggttttaaa ttttacaaaa gaattcgaga aaaaaacaaa tcctaaatta
     7381 tgctaaaagg ctttaaaatg tcaatgttat cattacgaat taggattttc ctatcgatga
     7441 tatttttgat attgatggct tcgattttga tggcttcgat ttcgataatt cagtttaaaa
     7501 acgaagcaaa agattaccac caagaacgat tagaccgaaa agaatttgct ataaaagaac
     7561 acataaatta tgtgctttca acaacaactt atcctttaac acctgaaaat ttaccgttaa
     7621 tttttaaaga taaaattcac gaattagctg atattcataa tctagaaatc aacatttatt
     7681 cccttgacgg aaaattgctc aaatcatcta aagccaagtt ttctgtagat aaggtttcgc
     7741 caccaattga taagtttatt ttaaaattag tgcaatcttc ggttgaaaaa cgatatgttg
     7801 acatcaaaaa tatcaatggt gtaaaaaatc gttcttcgta tagtcaaatc aaagatgaaa
     7861 agtttaaacc attggggatt ttgaacattc cttatgtgga agacgatggt ttttatgaag
     7921 aagaattgaa gcaattttta cttcgtttga gccaagtgta ttcgtttatg ttggttattg
     7981 cttttgcctt agcttatttt ttagcaagtt atataaccaa atctttaaag acgatttccg
     8041 ataaaattaa cgaaacgagt ttaaatcaaa aaaatgagaa aatcgtcatt gaagccaata
     8101 gtcgcgaaat taattccttg attgctgctt ataacggaat ggttgataaa ttggaagaaa
     8161 gtgcgagcaa attggcacaa agtgaacgcg aacaagcttg gcgtgaaatg gcaaaacaag
     8221 tagcacacga aattaaaaat ccgttgacac caatgcgatt aacggtgcaa agtttccaaa
     8281 gaaaatttga tgctaatgat cctgaattaa agcaaaaact agatgattat tcaaaaacat
     8341 taatacaaca gattgatacg atgagtgcag ttgcttcggc attttcaaac tttgcaacga
     8401 tgcctgcgca acaaaatgaa agtttgaatg ttgtagatgt agtagaatta tctttagaca
     8461 tttttaatga agattatatt gtttttgaaa gtaataaaga agaaattatt actaaattag
     8521 accgaacaca actcattcga attatcacca atttggtgaa aaatgccatt caatcgatac
     8581 ctgaagaaca aaaagaaaaa agtgttttgg tttcggtaaa tgaagtagac cataatgtaa
     8641 tcatttctgt aaaagacaac ggaattggca tcgaattata caaccaaagt catatttttg
     8701 aaccaaaatt tactaccaaa actagtggaa tgggtttagg tttgggaatt attagaaaca
     8761 ttatcgaaaa ttacaaagga actattactt ttgaaacaga attcggaaaa ggaactactt
     8821 ttattgtttc acttccaata attaattaaa atttaaaatc atgacctacg aaaatattct
     8881 aacctcaaca gaaaacggaa ttggacaaat caccatcaat cgtccatcaa aattaaatgc
     8941 attaaacgta gccactattc aagaattgca cgatgctttt gatactttag ataaaaattc
     9001 agaagttaaa gtgattatca ttacaggtga aggtgaaaaa gcatttgttg ctggcgcaga
     9061 tatttcagaa tttgctagtt tttctgtaga agaaggtgca caattagctg cacaaggaca
     9121 agaattattg tttgattttg tagaaaattt aaaaactcct gtaattgctg ccgtaaatgg
     9181 ttttgcactt ggcggcggat tggaattggc gatgtcgtgt catttcagag ttgcatcaga
     9241 caatgccaaa atggggttgc ctgaagtttc tttaggtgtc attccgggtt atggcggaac
     9301 gcaacgtttg ccacaattgg ttggaaaagg tcgcgctatg gaactaatta tgactgctgg
     9361 aatgattgat gcagaaactg caaaatcgta tggattagta aatcatgttg taccacaagc
     9421 ggaactttta gaatttacca aaaacattgc atccaaaatt atgcgcaatt ctccaatggc
     9481 aatcggaaaa gcgatttcag cagttaatgc gaattataaa gatggtgtaa atggctttga
     9541 aaccgaaatt aaaaactttg gaaaatgttt cggcaccgaa gattttagag aaggaaccac
     9601 ggcgttttta gagaaaagga aagcaagttt tagtgggaaa taattccaat gttttatgaa
     9661 aaaaaagcgg ttcttcatat tttgtatgct tatccaaata tcatgtgtgt tttcacaaaa
     9721 cataaactat aaaaaagtta aaagtgatat ttttcaagat gattataaaa attcggtaat
     9781 tgttctttcc gaagaaaata ctaatggaga attattgctt gttcgttctt ataaaggaac
     9841 aattgtttca caaggcgaag gcttttatat tgaatattat gacacaaatt taaagtttaa
     9901 aagagctttt gaatattcta tgaaccatcc aaattatcaa aaatacaatt tgatacttgg
     9961 tgtttttatg atgcaaaata atattcattt tgttgaaatt tattatgatt taaatgaaaa
    10021 atcatttgtt tgtctggata atattctatc tcaagacttt agttctacta aaaaagaatt
    10081 gtttagaatt tcaagagatg aaatgaaaaa ctatggaaaa ttcaatttac aacaaaaatt
    10141 ttatgacaga accaaagaaa tttggacaaa cgacaatagt ggcaaaatta actccgagaa
    10201 tgaattagca aatttaagtg gatttcataa agtatttttt tcaggaaata ataactataa
    10261 aaaaattata gaaaatgaaa accaaggaaa tggttcggat ataattttgg ttgtaaatga
    10321 acaaaaaaac agttttttag tggcattaga ttttaatcaa gaagaaaaag atggattaaa
    10381 actatactta tacgatgata agggaaacaa aaaatttgaa acaatatata gcaataccat
    10441 tgaagacaaa aagtattttt ttcaaaacct ccaaatatca gaagatggaa atttcatcta
    10501 tttattagca aaaacatatt cgtttgatgc aaagaaaaaa aatgaaggag gaaaatattt
    10561 ctatgaattt actaaaattt caaactataa ccaattaaca actacactaa atactaacga
    10621 acattttgtc ggttctctaa atacttttat acataatgac gagttaactt gtctaggttt
    10681 ttattcggat gtcaaagact ttaaatacag tggaatttgc tgttttaaaa tagacccaaa
    10741 tactttagaa gttaaatcag ctaattacaa cccatttaca gaacagtttt tatttgataa
    10801 atacggtgat agtaaaccca aagatttaaa aaacttaaat attaaaaaag tattttttac
    10861 tgaaaacaat gaaatcctac taaatgcaga agagaattat atttctcagt caggtgttgt
    10921 tggatatagt ggtgcaaaca attcgccaat gcaatttgga aattcagata aatattattg
    10981 ttatgatgat attgtcattg gaaaattaga tgctttaggt acgttacaat gggctagaaa
    11041 catcaacaaa aaacaatcaa ctaatgatga cgacaattct tttatttctt atacttcaac
    11101 tataagaaat aataaaaatt atctttttgt aaacacaaaa gacaaaatca aaaagttaaa
    11161 aaatgataga attgaatttg gtcaaattag aaaaaataaa tccaatttaa atgttattca
    11221 ggtagatgaa aatggcgatt ttgagtatga agaaatctta gacgacgaac aaaatgaggt
    11281 tccgtttatg gtttctaaag gtgccattat tgataattcc gtttattttc ttggtcgaaa
    11341 aggaagaaac aagcaattgt tgaaagtgac attgtaaaat ggtaaaaact attttcttat
    11401 tatttctcac ttctttttgc tttagtcaaa aacaaattac ctttatagat agcctcacaa
    11461 aaaggccaat tggttatgct tctatatgga aagaaaatca aatatataaa acgactgatt
    11521 ctctgggtgt tttaaatatt gaaaatgatt tattagataa gacttttaaa ataaccgcat
    11581 taggatacaa agaaaaagtt tttaaaatag tgacaaatga agtatccctt tcacctgaaa
    11641 ccagtctttt aaatgaggtg aaaatttata aacgtaaaaa accataagta aaaaaatagg
    11701 caacttaaaa cgctcaggtg ttcttttctg cactcaatat gatgctaagg aatcaagatt
    11761 tgcacactat tttcctaata gtcaaaatga acttgttttc cttgataaag taaaattaca
    11821 cacttctact acggcaaaaa acagaatttt ttctgtttta ttctactcag ttaatgaaac
    11881 aactggtgaa cctgataaag ttctcaatac tgaaactatt atttgtagac caaaaaaagg
    11941 aaacacacta aacactattg atgtttcata cgtgaatgtt gttattccaa ataatggaat
    12001 ttttatagtt attgaagttt tacttttaga acaaaacaag aattacgata aaaaatcaaa
    12061 tcatcctctt gcctttatgt acgaaccttg ctttaatgca aattcagttt cagagttcaa
    12121 agattcatgg tattttaaag ataacttatg gcaaaaaagt atccgtttat catataacct
    12181 tgctatagaa atttctaatt aatctactat ttctctcctt cccattcagc ataaaattgc
    12241 gacaaaaaat tttccatgaa tttgtgacgt tctaaagcaa tttttttacc agtttcggtg
    12301 ttcattttat ctttaagtaa gagtaatttt tcgtaaaaat gattcagagt tggtgcatcc
    12361 gaatttttat attcttcttt actcatagtg agttttggcg caattgctgg attgtaaatt
    12421 tgacgatttt taaatccacc ataattgaat gttctagcaa taccaatggc accaatggca
    12481 tctagtcgat cagcatcttg cacaatttct aattcgatgg aattgaattt cttgtcaaaa
    12541 tttccgcctt tgaacgaaat attttgaatg atattgataa catgagtaat gatttcttca
    12601 gaaacatttt gactttctaa aaaaattcga gcagtttttg gtccgatagt ttcatcgcca
    12661 ttatgaaatt tactatcggc aatatcgtgt aataatgctc ccagttttac aatttctata
    12721 ttacatgttt cgctttcggc tattaataat gcatttctat aaacccgttc aatatgaaac
    12781 caatcgtgtc cgccttcggc agtttttaat tggtctttta caaacgaaat ggttgaatca
    12841 attagtgaca atttggaatt tggaatttaa aaatttggaa tttacacaat agcaggttcg
    12901 acccacttaa aaatttttgt ctcggctggc aatactaatc gctctgatat ttttgccatt
    12961 ctttctggca atttcatcaa ataatcacgt gctttttcag cttctgcagt aagatttgtg
    13021 attttatcaa tttcccattt ttcggtaagt ttctttaaaa tatctacata atcactcgct
    13081 gtataaacgc ctatcttttg ggcagaatta gaaaattctt caaaagcagt gcctattttt
    13141 tctccagatt ctcttaagaa atgagctggc attgtgattt tttgtttcat catatactga
    13201 aaagcaagca tcatttcgct tggatcaact ttaaaaattt gagttacaaa ttcagaataa
    13261 gcgtgatgat gacgcatttc gtctccagcg atcattttac acatttttga cagctgtttg
    13321 tcgccatatt gttttgccaa ttgggaaact ctattgtgag aaatataagt agctaattct
    13381 tgaaaactag tataaacaaa gtttttataa ggatcttttc cagtgccaat atcaaaacca
    13441 tcattaatta aatgttggct ggttatttca acttcacgca tgtttactcg acctgataga
    13501 tacaaatatt tgttcaacaa atcgccatga cggttttctt ctcctgtcca ttgacgcacc
    13561 catttagacc agccatttct gccttcatta tcaatgcctt ctacttccat caaccatgat
    13621 tcataagttg gtaatgcttc ctcagtaata gtatctccaa ccataactac ccaaaaatca
    13681 tatggtaaat ctttagcaat ttctctaagt tcttttactt cttcaaaaaa agtttcgcct
    13741 tcagaattag gcaaaaaatc agaaggctgc catatttttt ctactggaat taaaaattga
    13801 tctacaaagc tatctacttt gttttctaga aactgcatga cttctagacg catatttttc
    13861 tgtgacatga tgatacgatt tgggattttt tactaaaaaa tcaaaattat tatttaatgg
    13921 atgaaactac tgattgctct gtttttttta tcaattcttc aaaagggaaa tctttaacag
    13981 gaatcgcttg gtgtatgata aattcaagtc tgttgcctaa acccattggg aatgctccaa
    14041 atttaaccat tttccatgaa ttattaatcg aaattggtac aacaaatgcc gaaggcgatg
    14101 atttgcataa gatttttagc ccactttgag caaattcttt tggttttccc gttttgcttc
    14161 gtgttccttc ggggaaaatt actgctgacc gattgtattt ttctatatac tctcctagtg
    14221 atttaattac tggaatggct tgttttgggt cttttctgtc aattaaaacc gaacctccat
    14281 gtcgtaaatt atatgaaacg cttggaattc ctttaccaag ttctatttta ctgacaaact
    14341 ttggatgaaa ctttcttaaa taccaaataa tcccaacaat atcatacata ctttgatgat
    14401 tggacacaaa aataattggc gcattttttg gaatagtatt tctgttttta aaagtataag
    14461 ttgttccaag tatattggtg catttcacta aaaaaaagtt taaataatca acactttttt
    14521 tgtgtgcttg atacccaaaa acattgagac aaaaccactg aataggatga aaaataacca
    14581 aacaaagtcc aaaacacaaa taatatacga ccgaaatagg atacgaaatt agtttttcca
    14641 tactcataaa ataggaaaca aaagtaagaa ttaaaattgg ctttagattg tttataattc
    14701 ttaaattatt attgtaaagg catagaaaaa aaacttattt ttacgaaaat taagcacttt
    14761 aaatatttaa tgaaatgagt tcagaaaaag aagccaaatt aaaagcatta caacttacac
    14821 ttgacaaatt agataaagct tacggaaaag gaaccgtaat gaaaatgggc gataaagccg
    14881 tggaagaagt agaaactatt tcttctggct cacttgggtt agacttagct ttgggggtaa
    14941 atggttatcc aaaaggaaga gttattgaaa tatatggccc agagtcttca ggtaaaacaa
    15001 cattgacatt acacgcaatt gccgaagctc aaaaagcggg tggcattgct gctttcatcg
    15061 atgctgaaca tgcttttgat agaaattatg cagaaaaatt aggtgtagat attgaaaacc
    15121 taattatttc acaaccagat aatggagaac aagctttaga aattgctgaa aatctgattc
    15181 gttctggtgc tattgatatt gtcgtgattg actctgttgc cgcattaaca ccaaaaagtg
    15241 aaattgaagg cgaaatgggt gattctaaaa tgggattgca cgcacgattg atgtcacaag
    15301 ctttaagaaa attaactgga acaattagca aaacgaattg tactgttttt ttcatcaacc
    15361 aattaagaga aaaaattggt gtaatgtttg gaaatcctga aactacaact ggtggaaatg
    15421 ctttgaaatt ttatgcttca gttcgtttag atattcgccg ttcatctcag ataaaagatg
    15481 gcgaaaacgt aattggaaac agaaccaaag ttaaggttgt taaaaacaaa gtggcaccac
    15541 ctttcaaaac agcagaattt gatattatgt atggtgaagg cgtttcaaaa acaggagaaa
    15601 tccttgattt ggcggttgaa tttgaaatcg tcaaaaaagc aggttcatgg tttagttatg
    15661 gtgacacaaa attaggtcaa ggtcgtgatt cggttaaagt tttaataaaa gacaatcccg
    15721 aattggctga tgaattagaa caaaaaataa aagaatatat aaaagaaacc aattcttaaa
    15781 aattaaaaaa accgtctcgt taaaaaaaag gcggtttttt ttatacttta acgcaacctt
    15841 ttatttgaat taacatccta aaacaaacat ttaattgaat tatgaaaaga gttttatttg
    15901 cattactatt aagcttaacg cttttatctt gtagtctcga tgatggcgat aaactttatt
    15961 tagaaattgt tcctgtcagt gctgtagaaa tgccaacagc ttttagagtg gatagtatta
    16021 cccaaatacc aatttcttat atcagaccaa cgccttgtca tgaattttca aatttttatt
    16081 ataattctat tggaaacgaa agaacagtag cggtatattg tttaacaaat ggaaaccaaa
    16141 actgtgtgcc aagcacggat tatgaaataa cagttccttt gaattttaaa ccaaaggaaa
    16201 tgggaacgta tcattttagg ttttggattg gcgaaaatga aaatggtgtt gaccaatata
    16261 tagaacatga aataacagta gatcactaat tatatttcgt ttgagtatag aagaactcat
    16321 acatggttgc aaaaagaaag acataaaagc acaagaacaa ttatatagac aatatgcgcc
    16381 taaattgttt tctgtgtgct taaaatattc tcgtaactat tccgaagcac aagataattt
    16441 gcaagatgga tttttactga tttttgaaaa aattaatcaa ttcgccttta aaggtgcatt
    16501 tgaaggatgg ataaaaagag tgatgattaa cttcattctt caacaatatc gaaaggaaac
    16561 atttttgaat ttagttcatg aaaattttcc taaagaaatt gaaattgaaa tagagaatga
    16621 atcagaaatt ccaattgaat ttttgatgaa aatcattcaa gaattacccg acaggtatcg
    16681 attagtgttt aatctatatg taattgacgg ttattcgcat caagaaatag cagatatgct
    16741 ttcgataaat attggcacat caaaatccaa tttatcaaga gcaaaagtga ttttaaaaga
    16801 aaaaatagaa accataacag gttccaaaaa tattccttca ataaaatgaa agaaaacaaa
    16861 aatatagacc aattctttaa agataaattc gaaaatttcg aaattaatcc accagaagaa
    16921 atttggaaaa acattgaaga aaaacttcaa aagaagaaaa aacgaagagt aatccctttt
    16981 tggtggtttg gctctggtgt tgcagcattg tttgtaattg gcttattcat ttttaacaat
    17041 aattatacag tagaaattca ccctgtaaat gatgttgtaa ttgaaaacaa tactcctaaa
    17101 aaaacaaact caatacaaga aaacaacaat gaaaatcaag gtattataaa agaaaatact
    17161 gaaatagtaa cttctgaatc ggataaaaac aaagcgtcgg aaaaaatcaa tactgaaact
    17221 aacaaggcat ataaagaaag ccttaattca actgaaaatt ctagtattgt ttcatccgaa
    17281 aagaaaagta caataatata taacgattac aaaaattcaa ttaaaaaaga aaaaaacacg
    17341 atacaaaact caaacaatac tattgtaaac aacacaaaat caagtgctaa aaaaaataac
    17401 aaaatttcaa ataaaagtaa tgttgacatt aaatcaaaaa ttactgaaat agaaaatgga
    17461 actgttgaaa aacaagaaaa tatagttcaa aataattccc gttttaatga aactaaatcg
    17521 gaattgaaca aagaaaacaa cattgaattg ggaaaaattg ctatgaaaaa tcataacaat
    17581 gttgctacaa aaggtgaagt aaatacttct aaagattcta ctgcaattgc tacagtagaa
    17641 actaacgaat tggaagaatt gctaaaagaa aaagaaaatc agattaccaa agaacaaaaa
    17701 atgaataggt ggcaagttac acctaatctt gctcctattt attttggttc atttgccgat
    17761 ggttcgccat tggattccag tttagacgag aattccaaaa gttttaacaa caatctaagt
    17821 tatggtgttg ctgttaatta tagcatcaat aaaaaaatta aaattagaac tggaataaat
    17881 tcattttcag ttgattataa taccaatgac atcatttttt ttcaaagcac taatgctaga
    17941 atgatgaaaa atgttactcc aactgttgaa ggttcattaa tcgaaattat tccaataaaa
    18001 aatcttaacg ctgctttcaa cagactagaa acagaacaaa atgatggtgt tttaaaccaa
    18061 cgtatgggat atatagaaat gccaatggag atttcctata aattactaga caacaaattt
    18121 ggcgttgaac ttattggtgg aatgagtgca ctttttttaa ataaaaacga agtatatctt
    18181 gaagcagatg gaatcactct taaagttgga gaagcttcta atttaaaccc aatgcatttt
    18241 agtggcaact ttggtgttgg ttttaattat ggctttttca aaaaatttca agctcgaatt
    18301 gaacctactt tcaaatatca attgaataca ttttcaagag attcgcaaga ttttaaaccc
    18361 tatatttttg gaatttacac gggtataagt tataattttt agtttgttaa gtttttaaaa
    18421 attatttagt tggtgttatt actttcgagc aataacagaa aagagttcca agtttttttt
    18481 ggaactcttt ttatttacta tcattctgaa gttcatttaa aatcaaagtt cgaacttcag
    18541 tttttaatgt ctttttaatt tctgactttc cttttgtttc aataaaatga tgcactttgg
    18601 ctctcaattg cccaggactt ccgctaaaaa aagtatagga aaatcgtttt ttattgtcgt
    18661 aaaaagtcat cggaacaact ggaatttcgt gttcaagagc caaacgaaat gcgccatctt
    18721 taaattcatc caaaacaata ctttcatcag gaacgccacc ttcgggaaaa atacaaatac
    18781 tcattccttg ttttaatctt ttttgagcac gttcaaaaac ttccattctg cttttaggac
    18841 tacttctatc aactaaaata caggttcttt tataaaaaaa tccaaataat ggaatttttg
    18901 ctagttcttt tttgcccaca aatacaaaag gattttcaac tatcgaaagc atcaacataa
    18961 tgtcaatcat tgaagtgtga ttggccacaa acatataact tctccctttt tctggatgtt
    19021 gttcaaaaac aactttggtg ttgaaaccca ttcctataag aatacatttt gcccaaattc
    19081 gagccatcat aaaaaaataa ggataccatg tttcttttaa aatagaaaga aataaaaatg
    19141 gaaacatgat taagattgga attgccatca tgatgtaaaa ccatatgcgg tataatatcc
    19201 aaaaaatgta tttcaatact ttcatagtgc caaatgtagt aaatgcaatg gaaaatttaa
    19261 tacaaagcag taactttgcg tgaagaataa aaaaattatg tcaaaaattt taactggaat
    19321 ccaatctact ggaacaccac atttaggaaa tttattgggt gccattttac ccgctatcga
    19381 attatcaaaa aatccaaaca atgaatcatt cttatttatt gctgatttac attcgattac
    19441 acaaataaaa aatggcgatg aacttcgtca aaatacatac agcaccgctt cggtttggtt
    19501 agcttgtggt ttgaatgttg acaaagtcgt tttctaccgc caaagtgatg tgccgcaaac
    19561 agctgaatta tcttggtatt tgagttgttt tttcccattt caacggttga cattagcaca
    19621 ttcttttaaa gacaaagcag atagattgga agatgtgaat gctggtttat tttcgtatcc
    19681 aatgcttatg gctgccgata ttttattata tgatgcgaat gtcgttcccg ttggcaaaga
    19741 ccaaatgcaa cacattgaaa ttactcgcga tgtagcttca cgattcaatc atcaaatggg
    19801 cgaaaccttt gtgcttcccg aaggaaaagt gcaagaagaa accatgtaca ttcctggaac
    19861 tgatggtcaa aaaatgagta aatctcgagg aaatttgatt aatattttct tggatgataa
    19921 agcattacgc aaacaaatta tgagtattga aaccgacagc acgccgctag aagaacctaa
    19981 aaatcctgat acatgcaaag tctttgctat ttataaattg ttggcaaact cagaacaaat
    20041 agcctcaatt aaaaaaaaat ataccaacga aagtcgagat tttggttatg gtcatgccaa
    20101 gcaagcttta tttgaattaa ttgtagaaaa gttcaaaacc gaacgtgaaa aatacaacta
    20161 ctacatgaat aacttagaag aagtagattc gttattaaaa cttggtgctg caaaagcggg
    20221 aagtattgct gatggtgttt tgaatagagt tcgagagaaa ttggggttta attaatccat
    20281 tatcactctt gaatcaattt taatgtcgcc gtttttaaat gaaatcgtca catcatcgtc
    20341 aaaataaaaa tcagctttca acaagtcttt tgtttgtggt gctaattctt tgaaacggta
    20401 ttctacaatt gattggtttc tgatgaataa atgattggaa ttggtattaa acgatgccaa
    20461 attttttccg atgtaaatat taaaaacagc attggaagct accgaattta gaattgcgtt
    20521 ttcgccagct tttaaataat atgccgttac tggcattttt atagaatcat aaatgacatt
    20581 gttttcaagc aaaaccatat cataaacaga agtatttttt actttaataa aatcactact
    20641 ttcacttttt tctacatttt tataaaacgt ttcaaacgga ttttcgccag attttaatgc
    20701 agaatttatg gtaaagtttt taatttctga agcattaaat tcagataaaa acgtatgaaa
    20761 actatctatc ttaaatttca tttccgaata ataaggatct aacgttggct tggtatcgtt
    20821 ataggtttca tcaatagtat aatcatcatt actataatta tttgatttat taaataaatt
    20881 agaactagat ataaaaaaac caattctcaa caaaacaaaa gttccaacaa caacataaat
    20941 ccaagttcga tttttctttt ttggtgcaaa tggtttataa aactccgaac ctttttctct
    21001 gtaaacccta attttttctg caaaattccc gtctaaagca acataacttc cagttgctaa
    21061 gaatgctttt cctgccattt ctgaataaga attttgatta aacatattga ttaacaattc
    21121 aaaaactgaa tgtaattttt cttcaagttt aggtgtttta attttactta aaaaagcgta
    21181 aaaattactg tcttttaggt attcaatttt agataaatca ctatacggag gaattagttt
    21241 ggaatgagca atatccaaac aatcttttgc ttcaaataaa acatagtagg caatagtgtc
    21301 agggaaataa gggaaagcct cggctaaaat actggcttta taaaattgat tttcttcaaa
    21361 actttgattg aaaaaagctt taatttcact tccaaaaaac aaacccacaa aagttttcat
    21421 tttatcaaca tcattactaa ccgtttctgg tttaaaaaaa tctcttggtt ggtctttatt
    21481 ggtaaaaaaa ttatataaga cacgattttc aaaaatgcct ctaaatgatt ctgaatgaaa
    21541 ttttaaagca ttcaaaaaat ttgtaattga aatgtctgaa atcaattgat ttttttcttt
    21601 ttcaataagt aaaacgtttt caatagttga aaaatcatct tgcgacagtg caccgattct
    21661 agatttatca attgcaagcg tttcaaataa ttggatgata ttcattagag gatgaagttt
    21721 taaaaaacaa atgtaagtta ttttctaatg gatttatatg gcttcaaata attttccagg
    21781 caaaggtcga atgacgcctt tcaattccat attcaacaat attgatgaaa ttctaaatat
    21841 tggaaaactg cattcaatag caatactgtc tagaagttgt tttccgtttt tttgcaaata
    21901 atcataaatc aattgttcat cgttatccaa ggaaacaaaa agttgttttt gcactgattt
    21961 tgttggtttt gattccaatt cccaactaag attataaatt aaatcggcag ccgaagtcat
    22021 caaagtagca cgttgggttt taattaaatc attgcatcct tgactaaact tatcagaaat
    22081 tcttcctggc accgcaaaca catcgcgatt ataatcattg gctaggtttg ctgttatcaa
    22141 tgaacctcct ttttcagcac tttcgataac aatagttgct tcactcattc cagctacaat
    22201 tctatttctt ctaacaaaat tttctttatc aggatttgaa ttgctccaaa actccgttaa
    22261 aaatccgccg ttttcttcca ttttagctac atatttttta tgcactttag gataaatttg
    22321 atttaatcca tgcgctaaaa cacctattgt ttgcaaatta ttatccattg cagctttgtg
    22381 agccacaata tcaacgccat aagcaaaacc gctaacaatt attgggttaa aaatggttaa
    22441 atcttctatt agtttcttgc aaaattcggt tccataagaa gtcacttctc gagtaccaac
    22501 tatgctgatg atttttctag tattcaaatc aaaattacca cttgaaaaaa gcaaggttgg
    22561 cccatcgata caatgtttta atttttcggg ataagccgtt tcttgataat acaaaacatt
    22621 aaaattctct tcttcaataa attgaagttc tttggctgct ttttcaaaaa ctgatttgtc
    22681 ttttaatttt tttaaaagca tatcgccgat accgtctatt gattgtaact gttgtctttt
    22741 tgatttaaaa acttcttcgg ctgaaccaca atgatttatt agttttttgg caacaatatc
    22801 gccaacacca tcaactttga gaagtgctaa aacatgaaaa agttcattag gaatcatctt
    22861 tataatttga aatacaaatt aattcttttt tattgtaatt gttaataaaa tttgtgaata
    22921 tttttttgtc aatcctgttg attatctaaa tttgccacta tgaagattga acaatacatt
    22981 tctcaactat tataccgcta tcaatgtgta accgttccgg gttttggtgc gtttttaacg
    23041 gagattcaat cggcaaagtt ggacgaaaac accaattcat tttatccgcc aaaaaaatta
    23101 gtttctttta atccttatat taaaaacaat gatggattat tggcaaatca tttgtctaaa
    23161 gaagagaaaa taaattatga agttgccatt tcagtgattc aaaccgaagt ggaaaattgg
    23221 agaaaatcga ttcatgaatt tggacaattg accttcaaaa acattggtgg tttttcgtta
    23281 aattctgaaa acaatttagt ttttgtacct gctgaacaag tcaattatct taaagaatct
    23341 tttggattaa gtacgtttgt ttcaccaacc gtaaagcgtg aagaatataa agaaatagta
    23401 gaagaattgg aagaaaaagc tccaattgtt tttactcccg aaaaaagaag aaattattcg
    23461 tttttaaaat atgctgcaat tgtgcttctt tcggttggag tatctggcgc aattggttat
    23521 aaagtatatc aaaactacga acaagaaatt gctcaacaaa cccttttggt agagcaaaat
    23581 gttcagaaaa aagtaaatca aaaaatacaa gaagctactt tttttatcga aagtccatta
    23641 ccaagcgtaa cacttactat tccatcagag aaaatgcctt atcatgttgt agctggtgct
    23701 tttagtattg aaagtaatgc tgataattta gtagaaagat tacttaaact aggctataac
    23761 gcaaaacgaa ttgcgccaaa taaattcggt ttaaaccctg ttttatatgg aagttattcg
    23821 agttattccg aagcttataa agccatgaaa gaaattcaac aagccgacaa tccaaaagct
    23881 tggttgctga tagaagagtt ttaaaaatgt cctgagaaat caggattttt tgttttcagt
    23941 agttaccttt gcataaaaaa atagttatgc atccaaaacc agcatcagaa tcattaacca
    24001 ttttaaccga tttagtttta ccaagtgaaa ccaatccctt gaataatcta tttggaggcg
    24061 aattattagc tagaatggac agagctgcca gtattgctgc tagaagacat tccagaagaa
    24121 taactgtaac agcatctgta aatcacgtag cgtttaaccg ctcaattcct cttggaagtg
    24181 tggttactgt ggaagcaaaa gtttcgagaa cgtttagaac ttctatggag atttttttag
    24241 atgtttggat tgaagacaga gagtctggca caaaaactaa agcaaacgaa gccatttata
    24301 ctttcgttgc cgttgatgaa acgggtcgtc cagtagaagt tgcgcctgtt atcccagaaa
    24361 cagaattaga aaaacaacga tttgatgcag cacttcgtag aaagcaattg agtttggtac
    24421 tcgctggaaa aatgaagcca agtgaggcaa ccgaattgaa agcattattt atttaatttc
    24481 ttttttgtat tctaaaaaga agtatatttc gtgaaattta tttaccaatg aaaattactt
    24541 ttttttcgtt ggcattttta ctttttttcg caattgatgg taatgctcaa aaaggtttag
    24601 atccaacagc tgatgagatt tctcaagcta aaaaacttag agaaagctat tctaaggacg
    24661 acattgcaat tttggaaagt tccgaattaa tcactttcga tttaaacaaa aaagcagcca
    24721 aagttattgt caatcattcc ataaaagaac atttgatgaa catcaaccac cgtgctgata
    24781 ttcaaaaata tgagttctat gacagtgaat ctagcattca aaatttttct ttaaaatata
    24841 gaaacgataa aaccgcttcg ttttcggtaa aagatgagtt ttataaagat cgtgatttat
    24901 tttataatga cgccaaagta aaatatgtta ccattgattt tccagttcaa ggctataatt
    24961 acaaatatga aatggaaaaa aaatatgatg atgtaaaata ttttacttcg ctgtttttta
    25021 atgatgaata tcctgtttta tctaaaaaaa tcacctttga aatcccaaat tggttggatg
    25081 ttgaattgaa agaattcaat tttgacggtt ttaaaatcaa taaaacttct aatactgaag
    25141 acaaaaaaac tattgttacc tatactttag aaaatgttcc tgctgctttt aaagaaaatt
    25201 caatgccggg aagaagttac ctttatccac atattttggt gattgcaaaa tcatttacca
    25261 aagacggagc aaccaccact ttatttaaga gcaatgcaga tttatacaat tggtacaaat
    25321 cgttgattgc aatgatgaaa gatgacacta ctgttatgaa aagcaaagta aacgaactta
    25381 ctgccaatgc aaaaacagat gaagaaaaaa taaaaaacat ctattattgg gtgcaagata
    25441 atattcgata tattgctttt gaagatggaa ttgcaggttt taaacccgac gaatccaata
    25501 atgtttttga aaaaagatat ggcgattgca aaggaatggc aaacttgatc aaacaaatgt
    25561 taaaacttgc tggtttcgat gcgcgattaa cgtggattgg aaccaagcac attgcctatg
    25621 attacagcac accatcatta gccgttgata atcacatgat ttgtactttg atgtataaag
    25681 gaaaaaaaat ctttctagac ggaacagaaa aatacaactc ttttggcgaa tatgcagaac
    25741 ggattcaaaa caaagaagcc atgattgaag atggtgacaa atgtattatt gaaaaaattc
    25801 caactacgac tgccgatttt aacaaagaaa cttttaaaac aacacttgct attgaaaatg
    25861 aaactttaat aggagtttgt aacaaaacct atcaaggtga aagcagagcc gaatttttaa
    25921 atatttacaa cggttttgaa aacaccaaaa gaaaggaacg attggacgga tatttgtcaa
    25981 aacagaacaa aagcattgct gtttctgatg taaaaaccag tgatttacaa aatagagata
    26041 gcaaacttac cattgattat aacattgcaa ttcaaaacaa aatttcttca tttgatgacg
    26101 aaatttatgt tgatttggaa ttcatgaacg aatacaaaaa ctttgaatta aaagaccgta
    26161 aagtagctta tgaaatgggg tataaaacta attatgtgtc gcaaattaac cttaaaatac
    26221 ctgatggtta taacgttaca aaaatgccaa ccaatctttc aataaaagaa aatgattttg
    26281 acatcaactt aagttatgaa aacactggaa aagaaattat ctataaaaaa tcttttatgt
    26341 ttaaaaatgg tctaatcaaa gcagacgcaa ttgaaaaatg gaatgaattt aataaaaaat
    26401 taaaagaaaa ttacaaccaa caaatcacat tatcaaaata accaagacct aacttaatga
    26461 aaacaaaatt agccttacta ttctttttag ccattattgg catttgttct gctcaagatg
    26521 ccaaagaagc tcgcgagttt ttttggggtg caaaagatga atttaaaaaa gccaacacca
    26581 ttcccgataa atggaaaaac gaatcggccg taattattca taaacgcgaa cattataatt
    26641 atcataagtt tggaaaaagc gttacctatg tttcgggtat tcgaaaaaga atcatgttgc
    26701 ttgaccaagc cgcagtaacc gaattttcgc aattctcata taaaaataaa ttttattcta
    26761 atagagctta tggttataaa aaaggcacaa actatgttgg tgtaaaaata ataaaacctt
    26821 ctggtaaaga aattgaaatt gataccgata aagaatccaa agaagtagat ggtgaaaaaa
    26881 aattagccgt acccaattta gaaattggcg atattattga ctactatttt tatagtgtcg
    26941 aaccctttaa gtcatttttt gactacactt ttgaagccgt agaaactcca cttggcgatg
    27001 tatatccaac tatggattta aaaataactt tagataccga aaatgatttt tttattaatt
    27061 ttaacactta taacggagca cccaatttaa aagaaattcc aaccaaaaat agcggcgaac
    27121 gcagttatga actctctaaa aaagacattc ctaaaaatga atttccacgt tggttttatc
    27181 ctttagccga aatgccatgc tataaatttc aagtaatttt tgcccgttca ggaaaatttg
    27241 aaaaactagc cgatgggttt ctttccgaaa aagaacgcga cattaaaaag actgtttcca
    27301 aagaagatat ttttgattac tacaacaaaa agtttgttcc tgctggagat attggcgaca
    27361 tcaatgattt tttaaaagga aaaacattta cctctgaaga agacaaaatt cgggaagtat
    27421 attattttac acgtcatcaa ttctatactc gatatattga agcttttgtg gtaaacgaag
    27481 ctaagatttt ttatcctttt gaatattatg gaaattaccc tatattcttt aattcagaag
    27541 tgcaatttgt caattatttt atgcaattct taaggaaaaa tgatattagt tatgatgtta
    27601 ttattgcaac accacgctac gatggttcaa ttgatgattt gttaattcaa tctaatgtga
    27661 aaaaacttat tcgagtgaat actaaaaacc ctgtttattt ggaatatttt tcaccctata
    27721 ccagtgccga tcaatttaac tacaacatag aaaattcaaa agcctatgtt ttagaagtat
    27781 caaaaggaaa acgcataaca gatgcctcaa cagttcaatt accatcaagt acctatcaag
    27841 aaaacagtgt gaaaaccaaa accgaattaa ccttggagga ttttactgga tttaaagcca
    27901 aaaaactatc ttcttattta ggtcatttaa aagacagcga gcaaagtgac agacttaatt
    27961 tttatgatta tgtatatgaa gattacaaaa aatatgcaac aaaaccactt ctggattacg
    28021 taaaaaacaa gaaaaaaaac gagcagtaca gcaatgaaat gaaagcctta atcaataaaa
    28081 tgaaagacac tcaaaaagaa tcctttgaaa aatcaatcga aggagaactt gattttctcg
    28141 tggacgatta ttcttttaaa atcaacaata cgggtcgtta tggcaaaaaa tcttcttttg
    28201 aaatggaaga agagtttagc attaaagata atttgattaa aaaagctggc gataaatatc
    28261 taattgaatt aggtaaatta ttaacctcac aaattgaaat tgaccaaaaa gaaaaagata
    28321 gaaaaaacaa tatctattct gtttatccgc gctcgtatga gaatgaaatt attttaaata
    28381 ttcctgttgg ctattcggtt tctggtttag aaaaattcaa taaaaaagta gaaaacgaaa
    28441 cgggcggatt tacttcaact gccgaagtga aaggcaacca actcattgtt aaaacctaca
    28501 aacattataa aaattatttt gaacctaatg caaattggaa caaaatgatc gattttctag
    28561 atgctgccta tcagtttact caagaaaaag tgttgttgaa gaagaattaa ttctatcgta
    28621 ataataaaca taa
//
LOCUS       DAOOBH010000017        28277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_2191_length_28277_cov_7.385940, whole genome shotgun sequence.
ACCESSION   DAOOBH010000017 DAOOBH010000000
VERSION     DAOOBH010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 28277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28277
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_28277_cov_7.385940"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..2532)
                     /locus_tag="PK218_03550"
     CDS             complement(100..2532)
                     /locus_tag="PK218_03550"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HPW97616.1"
                     /translation="MYVVKRDGHKEPVMFDKITDRIKKLCYGLNELVDAVKVAMKVIE
                     GLYDGVTTSELDNLAAETAASMTVSHPDYAQLAARIAISNLHKNTNKSFSETMNEMFH
                     YVNPRTNLKAPLLSEEVHKVIQENAEFLDSHIIYTRDFNYDYFGFKTLERSYLLKING
                     KIVERPQHMLMRVSVGIHLNDLESVIETYDLMSKKFFTHATPTLFNAGTPKPQMSSCF
                     LLTMQDDSIDGIYDTLKQTAKISQSAGGIGLAIHNVRATGSYIRGTNGTSNGIVPMLR
                     VFNDTARYVDQGGGKRKGSFAIYLETWHADIFEFLDLKKNTGKEEMRARDLFFAMWTS
                     DLFMKRVEADTTWTLMCPNECPGLDNVYGDEFEALYTSYEAQGKGRKTIRARELWEKI
                     LESQIETGTPYMLYKDAANRKSNQKNLGTIRSSNLCTEIMEYTSPDEIAVCNLASISL
                     PMFVENGKFDHELLYNVTKRVTRNLNKVIDRNYYPVKEAENSNLRHRPVGLGVQGLAD
                     AFIMMRMPFTSDEAKQLNQEIFETIYFAAVTASMELAKEEGPYSTFEGSPISQGEFQY
                     NLWGLKDEDLSGRWDWKNLRKEVMKDGVRNSLLLAPMPTASTSQILGNNEAFEPYTSN
                     IYTRRVLSGEFIVVNKHLLYDLVERGLWNETLKQEIMRHNGSVQNIDQIPNDLKELYK
                     TVWEMSMKDIIDMSRHRGYFIDQSQSLNLFMQDANYAKLTSMHFYAWKSGLKTGMYYL
                     RTKAAVDAIKFTLNNDKKAEPIAEKPEAVAVEVVAPVENGEMTAEDFAAMVERARNAA
                     GTMGDDCEMCGS"
     gene            complement(2651..3628)
                     /locus_tag="PK218_03555"
     CDS             complement(2651..3628)
                     /locus_tag="PK218_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HPW97617.1"
                     /translation="MASIEPILQENKNRFVIFPIKHHDIWDWYKKMEASFWTAEEIDL
                     HQDLTDWNNKLSHDEKYFIKHILAFFAASDGIVNENLAENFVNEVQYPEAKFFYGFQI
                     MMENIHSETYSLLIDTYVKDEAEKAELFNALEVFPAIKKKADWALKWIESDSFAERLI
                     AFAAVEGIFFSGAFCSIFWLKKRGLMPGLTFSNELISRDEGVHCDFAVHLHNHHLVNK
                     VPKERIREIIVNALNIEREFITESLPVSLIGMNATLMTQYLEFVADRLLVELGCEREY
                     NSSNPFDFMDMISLQGKTNFFEKRVAEYQKAGVMSKDNDDQKISFDADF"
     gene            complement(3964..4446)
                     /locus_tag="PK218_03560"
     CDS             complement(3964..4446)
                     /locus_tag="PK218_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97618.1"
                     /translation="MKKVMLMCMMFVFSNIIFAQTTKEKVAKTKAKTEKKAEKAKEDL
                     DKKVAKAKKEQDKKVTEAKKEISKTKKETNEKVASAKKEIKAEESKSKTKINTAKAKK
                     TEVEEKEDMKETAASKTAEKKVKDKITGTYKGKKVYTGPKGGKYYINKNGNKTYIDRD
                     "
     gene            complement(4514..7912)
                     /locus_tag="PK218_03565"
     CDS             complement(4514..7912)
                     /locus_tag="PK218_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97619.1"
                     /translation="MHKIIFHCFLLISCFQLSAQEINSLYKTKKIAISKDTIEIEKVS
                     INPSFFKLETKNGKPIDSTFYKIDFPSGKLIFNKNAPISDTLVVQYLQYPDFLTKTYS
                     IYSDEVIVPNEDGNLYKVNREVKKFIPFDGLNTSGSITRGVTVGNNQNTTVSSNLDLQ
                     ITGKISDKVSLRASIQDSNIPLQSGGYSQKLDEFDQIFIELFTDNWNIRAGDLFLENR
                     QSRFLNFNKKVQGLSTHFTFGGEENKNDIFASAALVRGQYAKSSLTGQEGNQGPYKLR
                     GNNGELYVLVISGSERVYVNGILKQRGENNDYVIDYNAGEVTFTSLFPITSEMRIVIE
                     YQYSDRSYTRFVTYAGANHEAKKWSLGGYLYSENDVKNQPLQQNLSAEQVQVLQNAGD
                     DVNLMNAPSAYLDTYSENKILYKKVTVNGVEVFEYSNNPDDELYNVKFLLIGNNLGNY
                     ILSNSVSIGKIFQYVEPVNGVPQGNYEPVVRLVAPTKIQIATVLGKFNPNEKTLIDFE
                     VGVSNNDLNLFSNLDDDNNKGLAGKLNFKQRLLSKKWNIDVFGKYQFIQKDFRTIERL
                     FNIEFNRDWNLTTFSGNQSLLVNGLDFVLPEKGKLTYQYENLSFTESFSGNRHSINGF
                     FKLKNWNITQNGSYLKSNGSIASSTFARNQSQARYNFKKNWIGGVFNLEDNQEKNKIT
                     NQLSALSQRFSEYGAFVGRGDSTKVFVELGYLHRVNDSLQNGYLQKVNRSNSYYIKSK
                     LIQTDKSDLSVFVNYRDLKYTDNTRQNEPSLNSRILYNDRFFNQFLQTTTAYETTSGT
                     IPQQEFTYLEVEPGQGVYTWNDYNNNGIQELEEFEVAPFPDQAKYVRVFLPNQIFLKT
                     HQNKFSQSLTINPNNWQNETGLKKVASYFYNQTSFLIERKIQRNGNNFDLNPFSTSDT
                     NLLGLNTSFRNSLFYNRGKQRHSTTYTFISNRIKSLLSAGSQESKNSSHQLQYNHLVR
                     KTWLFTFATKTIETSLTSENYASRNFEVLGYQINPKVSYLFNKNASWDVFYEYQNKEN
                     QIGTLDKLLQSRFGTSFSYASDKRFTINGEISFYQNQFTGDANSPVAFQMLEGLQPGK
                     NSTWRLLLQKNLTEFLDINLNYQGRKSETSQTIHTGNVQLRAYF"
     gene            complement(7950..9203)
                     /locus_tag="PK218_03570"
     CDS             complement(7950..9203)
                     /locus_tag="PK218_03570"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPW97620.1"
                     /translation="MRIFKFGGASVKDADGIKNVYSVLEKVGHEDTLLVISAMGKTTN
                     ALEVVIKNYFDKSPELHSSLQEVKKYHNQILMDLFDDEDHDVFYTVNSHFADLEYFIR
                     SNKSPNYNFVYDQVVSYGEIISTTIVSHYFNHVGLKNNWIDVRNFIKTDNNYRDATVD
                     WDKTQGYISKGVKKKALNITQGFLGSDENNFTTTLGREGSDYTAAIFAYCLNAESVTI
                     WKDVPGVMNADPRFFENATLLNQISYREAIELAFYGATVIHPKTLQPLQRKEIPLYVK
                     SFLNPLLPGTSVSKGADLVPQTSCFILKKNQLLISLSSIDFSFIMEENISEIFKLLHE
                     YKIKVSLIQNSAISFSVCVEDKFGNFNELKSILSKKFKVSYNENVSLYTIRHFDEKAA
                     RMVETDKTILLKQISRGTLQLVTKE"
     gene            complement(9225..9707)
                     /locus_tag="PK218_03575"
     CDS             complement(9225..9707)
                     /locus_tag="PK218_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW97621.1"
                     /translation="MIIRKAHKEDMKAVLELIQELATFEKEPQAVEITVNDLVCDGFC
                     ENPLFHCFVAELENEIVGMALYYYRYSTWKGKTIHLEDLIVKEKMRGSGLGFALYSKI
                     IEQGKTDNVRRIEWAVLDWNTPAIEFYKKSGAKVFDDWRIAQMDEQGIFNFLTNKSKV
                     "
     gene            9814..10821
                     /gene="fbp"
                     /locus_tag="PK218_03580"
     CDS             9814..10821
                     /gene="fbp"
                     /locus_tag="PK218_03580"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HPW97622.1"
                     /translation="MEERNRTLGEFIIENQNAFQYSSGELSRIINSIRLAAKVVNYKV
                     NKAGLVDIVGAAGDQNIQGEDQQKLDVYANEVFIQTLINREIVCGIASEENDDFITVA
                     GSDKSHSNKYVVLMDPLDGSSNIDVNVSVGTIFSVYRRVTPVGTPVSSKDFLQPGTQQ
                     VAAGYVIYGTSTMIVYTTGHGVNGFTLNPAIGTFYLSHPNMQFSKDGKIYSINEGNYV
                     HFPQGVKDYLKYCQFEEDDRPYTSRYIGSLVSDIHRNMIKGGIYIYPTSSKAPNGKLR
                     LLYECNPMAFIAEQAGGKASDGFGRIMEIEPKELHQRVPFFCGSYNMVEKAEEFMAKY
                     K"
     gene            complement(10855..11286)
                     /locus_tag="PK218_03585"
     CDS             complement(10855..11286)
                     /locus_tag="PK218_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HPW97623.1"
                     /translation="MSFSDLFDSEFKNRNKGHFSAIVRVAIADGDLSKEEKEFLDKLA
                     IRLEISPAEYEEILENPLKYPINPPYLHSQRLERLYDLTRMVYADHILGPRQKEILTK
                     FALALGFTPGNVPYIVDKALSLLVMNVDLDTFVYEMTNMNK"
     gene            11438..12031
                     /locus_tag="PK218_03590"
     CDS             11438..12031
                     /locus_tag="PK218_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HPW97624.1"
                     /translation="MSDFWIYFNHGLKHILSFSAYDHVLFLLALVASYEFKDWKRLLI
                     LVTTFTLGHTLALFLSVFNIITVKSEWVAFLIPITILIVAVYNIVAAGKSFKKNTITF
                     IALVTAFFGIIHGLGFSSYFNTFVSGKPSDKLAPLLEFALGIEAAQIIVIILALLLAY
                     VVQTLLKFSKRDWILIVSAFILGVVVPMIVHNKILSN"
     gene            12124..12555
                     /locus_tag="PK218_03595"
     CDS             12124..12555
                     /locus_tag="PK218_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPW97625.1"
                     /translation="MKEKKQHKYDVAYLRIASEWSKLSYCKRKQVGAIIVKNRMIISD
                     GYNGTPSGFENCCEDDEGLTKWYVLHAEANAISKLAQSSQSSEDATLYITLSPCKECS
                     KLIHQSGIKRVVFQNGYRDDSGLEFLRKAGVIVEQIESLEV"
     gene            12560..14128
                     /locus_tag="PK218_03600"
     CDS             12560..14128
                     /locus_tag="PK218_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPW97626.1"
                     /translation="MKSKEKYLPILLFSCIGFGIMIGGMLNFPTQKLSSKNASQVKLN
                     RLINFIDNDYVDEVNTDSIVDLTVNSILEKLDPHSIYIPPSIQTSEAEIMKGDFVGIG
                     VNFYMHNDTVAVVSPLPNGPAEIAGIKAGDRILFADRTKLFGRNLPSDTLYNKLKGDD
                     GSDVLLTIFRKSTNEKLKFNVIRRTIPIKSVDIATLINPTTGYIKISRFAETTYDEFH
                     KGLTGLKNKGISTLIIDLRNNGGGYLERAVDIADDLLKDNELIVFTKNKKGTVNKTFA
                     TEKGIFENGKVFILIDENSASASEILAGAIQDNDRGIIVGRRSFGKGLVQREMDFEDG
                     SAVRLTTARYYTPSGRSIQKAYTKGEGEKYSHEFDTRFESGELYAKDKIKIADSLKFK
                     TKKGRIVYGGGGIVPDIFVPLEVKKGEEGVAYLLNTGVVGHFVFEQLDISRKKYKLMT
                     YTQFIASVNEDTTLVIKFEKYLSKSGFEVDLSSNKDIVKKNIVAEFVRQLFSDEQSFQ
                     ILLKEDPMIKAVLK"
     gene            complement(14135..14257)
                     /locus_tag="PK218_03605"
     CDS             complement(14135..14257)
                     /locus_tag="PK218_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97627.1"
                     /translation="MAFDIGIERGMIHTNIANVKFTPKVLDVSVAKLFEVDKRE"
     gene            14958..17330
                     /gene="pbpC"
                     /locus_tag="PK218_03610"
     CDS             14958..17330
                     /gene="pbpC"
                     /locus_tag="PK218_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HPW97628.1"
                     /translation="MPNRFQKLVRITSFFKNNLKKSIILIVLLSVYYFSLPKILFEEP
                     YSTVIESSEGELLAAKIARDGQWRFPAKDSIPDKFKKCIVYFEDEYFYHHPGFNLVAM
                     TKAIQQNRKAGKVVRGGSTLTQQVIRLARKNKKRTYFEKAIEIILATRLEFRSSKEEI
                     LNLYAAHAPFGGNVVGLEMASWRYFGVQSHQLSWAETATLAVLPNAPSLIYPGKNQIK
                     LQKKRNALLMKLFDEKVIDKLTYELSIQEPLPQKPYDVPQIAQHLLQKVAKTDEGKRI
                     KTTISYPIQNRANQIAQYYYNQYKQNEVHNLAILVIDVQNRNIISYVGNSPTDKEHNK
                     DVDIISAPRSTGSILKPLLFASMIDDGELLPNTLVADIPTQISGYTPQNFNNSFDGAV
                     PAQRALARSLNIPAVLMLQEHGVNKFYELLQQFKLRNINKLPDHYGLSLILGGAESNL
                     WDLCRTYSNLTGTLNYFNQSNGKYRSNEFVELNYRNGFKQDFGTSGFQKNILSAGSIY
                     LTYNAMKEVNRPEGDEAWKFYDSALQIAWKTGTSFGNRDAWAIGTNSRYVVGVWVGNA
                     NGEGRPELTGVTNAAPILFDIFNVLPRKNWFQPPLNDLETVEVCKQSGYLAQEDCPKV
                     KQLITKIGKKTAICPYHKWVHLDKSEQFRVNSDCESVENIVNKKWFILPPVMEWYYKS
                     QHIDYKTLPPYRSDCQQYQQASMDFIYPKPNSKIYLAKDFYGKIQPVVLKIAHSNRES
                     KLFWSLDNQFLGETQTFHEMPIKPETGIHYITVTDEFGNEIKRKIEIINE"
     gene            complement(17451..18989)
                     /locus_tag="PK218_03615"
     CDS             complement(17451..18989)
                     /locus_tag="PK218_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HPW97629.1"
                     /translation="MNKELIIRSSEDAVDFALLKDGKLIELHKQEDTSNFLVGDIFIA
                     KIRKPVAGLNAAFVNVGYEKDAFLHYHDLGPSFNSYMKFIKLVSAGKLKDFSLKNFQF
                     EKEIDKNGAITDLISANQSVLVQVVKEPISTKGPRISAELSFAGRYVVLVPFSDRVSV
                     SQKIESKEEKDRLKKLVQSIKQKGFGVIVRTVAEGKSIAELEKDLQSLMNRWTAMCKK
                     LPTAHHPSKVLGELNKASAILRDVFNDTFTSILTDDKELYQETKDYLAEIAPEKQKIV
                     KLYQSNDVPLFEKYHIERQIKTSFGRTVSMSKGAYLIIEHTEALHVIDVNSGNRSNKA
                     SNQEDTALEVNMIAAAEIARQLRLRDMGGIIVVDFIDMQNPDNRKVLYDFLKLEMDDD
                     KAKHKILPPSKFGLVQITRQRVRPEVNIETREENPNNLSSDVEAPISIIDNITSDLEK
                     IIKDHKKIKLNVHPFVAAYLKKGFPSLRSKWLFEHKKWVKIIPRDAYTYLEYHFFNEE
                     GKKL"
     gene            complement(19406..19699)
                     /locus_tag="PK218_03620"
     CDS             complement(19406..19699)
                     /locus_tag="PK218_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPW97630.1"
                     /translation="MTKADIVAKISERKGLEKQDVQAVIEAFMEEVKTSLETGDNVYL
                     RGFGSFIIKTRAEKTGRNISKNTTIKIPAHNIPAFKPAKIFVESVKTNTEIPV"
     gene            19833..20873
                     /gene="mutY"
                     /locus_tag="PK218_03625"
     CDS             19833..20873
                     /gene="mutY"
                     /locus_tag="PK218_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPW97631.1"
                     /translation="MNFSNALIVWYLQNKRNLPWRNTLNPYLIWLSEIILQQTRVAQG
                     LPYFLSFAERFPTVFDLANADEEEVLKLWQGLGYYSRARNLHKTAKTIVFDLNGKFPK
                     NYNELLKLKGVGEYTAAAIASFAYNENVPVVDGNVFRVLARYFDIETDIASSNAKKEF
                     RALASKLLPNGKANQFNQAIMEFGALQCVPKNPNCEICVLNNSCLALKNKKVTQLPVK
                     LKKTKIQKRVFNYLIFRDEDKNTLLQKRTQKGIWHNLYEFPLIETFEDINDFETLELI
                     QNQSFIVNEIQNIRLINPVKITHKLSHQHLSVSFWEVSVAGKIKDGIDCDSVLKFPFP
                     IVIYNFISKIWE"
     gene            20917..21369
                     /gene="ssb"
                     /locus_tag="PK218_03630"
     CDS             20917..21369
                     /gene="ssb"
                     /locus_tag="PK218_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPW97632.1"
                     /translation="MSSGTLNKVMLIGHLGDEIKMHYFEGGNCIGRFPLATNEVYINK
                     QTNEKITSTEWHNLVVRNKAAEICEKYLSKGDKIYVEGRIKSRQWQAEDGTFKYTTEI
                     QVTEFIFLNTKKDIDGTKNIQNTDSKNTTFELPKPENDLPNLDADMPT"
     gene            21397..22692
                     /locus_tag="PK218_03635"
     CDS             21397..22692
                     /locus_tag="PK218_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HPW97633.1"
                     /translation="MDTEPSLNFIETVDTNIILGSIGVFILLFFSAMVSAAEVALFSL
                     TPNDLNKLNEENPRKGDLLSKLIQKPKKLLATLLLANNFLNIGVVILFSMVGDTLFSG
                     ISSPILKFVVEVILVTFLILLFGEVLPKVYASRNNVKFSVALSTPIFILDNLLSPISL
                     PMRNITVFLHAKFGNQKSSLSVDQLSQALELTSDEDTTQEEQKILEGIVSFGNTDTKQ
                     VMSPRIDVFGLDIDESFADIYPKIIEKGYSRIPVYQENIDDIKGVLFVKDLIPHIHKK
                     EFDWKKIIREPFFVPENKKLDDLLKDFQSMKSHMAIVVDEYGGTSGLVSLEDIIEEIV
                     GDISDEFDDENINFSQIDDRNYLFEGKITLKDFYRIIDVDEDLFESKKGEAETLAGFI
                     LENLGNFPKKGQKIHFSNLFFTIETVDKRRIKQIKVTLE"
     gene            22692..23255
                     /gene="gldD"
                     /locus_tag="PK218_03640"
     CDS             22692..23255
                     /gene="gldD"
                     /locus_tag="PK218_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HPW97634.1"
                     /translation="MKINFKHILVLVIMVLFFSCKDDVLPKPASQLRLDYPLAEYVFY
                     SDACPFTFDVNDEVDVKEDKNCGFSIHYPKMKATIYLTYKPVSNNIDKLLRDAQKLTF
                     EHVIKADAIQEQPFINADSKVYGMFYRVNGNAATNAQFYVTDSVKHFVTGSVYFYAKP
                     NFDSIMPATSYVRDDMQHLMESLQWKN"
     gene            complement(23312..23695)
                     /locus_tag="PK218_03645"
     CDS             complement(23312..23695)
                     /locus_tag="PK218_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HPW97635.1"
                     /translation="MKFTSSIFTLALCTLFLTGCKETNSKAESQTNASDSVTIESKKT
                     IATKPETASFTIDGMSCAIGCAKTIEKKLSSTEGVQDAKVDFDKKEATVNFDAAIISP
                     EKIQEIVEKTADGKTYKVSDVKIKV"
     gene            23811..24677
                     /locus_tag="PK218_03650"
     CDS             23811..24677
                     /locus_tag="PK218_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW97636.1"
                     /translation="MKSLQIKWVYLIVLALIWGSSFILIKKGLVALSPFQLGSLRIIF
                     SSIFLLILGFKSLPTIPLHKWKFIALTAFLGTFFPAFLFAFAQTQISSSVSSILNSLT
                     PLNTLLLGGLFFGVAFKRTQIIGVFIGLCGTLLLILNGAVHHPNQNYFYTILVIIASI
                     CYATNVNLIKKHLSDIKPLAITTGNFFVLLIPASIVLYFTNFCSIISEVEVQNATVYI
                     LILGILGTGIANIIFFKLIQISSPVFATSVTYLIPIVAFFWGLLDGEFLTPVQVIGAF
                     IILVGVYMSSKK"
     gene            complement(24733..26787)
                     /locus_tag="PK218_03655"
     CDS             complement(24733..26787)
                     /locus_tag="PK218_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPW97637.1"
                     /translation="MKKIAIILSSLFLTITMQAQDRTQPKPGPSPKINIKKPETFSLP
                     NGLKVLVVEDHKLPRVSYSLTIDNAPYAEGNKKGVSELTSSLLGNGSTKTPKDKFNEE
                     IDFLGANINFYASGASANGLSKYSKRILELMAEGALMPNFTQEEFDKDRDKLIEGLKT
                     QEKSVSAAANRVQDVLAYGKNHPSGEYLSEETINNVSLADVKNNYRTYFVPENAYLVV
                     VGDVKPKEIKKMVESLFGSWVKATAPRLTYSNPKNVQYTQINFVDMPNAVQSEIALVN
                     TVSLKYSDPDYFPVIVANQVYGGDFNSYLNMNLREAHGWTYGARSYVGNDKYGDSKFA
                     ASTQVRNTVTDSAVVEALKELKKIRTEKVDETVLKNVKAGYIGRFVMQVEKPATVARY
                     ALNIQTEGLPADFYENYIKNINAVTPEDIMRVANKYMLADNLRIIVVGKATDVLPGLE
                     KLKIPIFYFDKYGAPTEKPAMKKAAPAGVTAKSVIENYIKAIGGEKAVMAVKTVSITG
                     SATIPQAPAPIKMVIKKDNKGRSLLVISMDGMGELNRQVVTEKGGYSSAQGQRKEMTA
                     EEFAEEKETAATFDELVLINNPNLVLDGIEPMNGTDAYVIKNGKTTLYYDVKSGLKLS
                     ESKLEEQGDKKMTVTTAYSDYKDVKGVKIPHAITINQGFELNFIMSDVKVNEGVTDAD
                     FK"
     gene            complement(26802..28127)
                     /locus_tag="PK218_03660"
     CDS             complement(26802..28127)
                     /locus_tag="PK218_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPW97638.1"
                     /translation="MKKSLIALSTMLMLSGITSAQKVAFEEYDLDNGMHVILHNDPSA
                     PVVITSVMYHVGSKDENPERTGFAHFFEHLLFEGTKNIGRGEWFKIVTANGGTNNANT
                     SDDRTYYFEVFPSNSLEVGLWMESERLMHPVINQIGVDTQNEVVKEEKRLRVDNQPYG
                     NLIAQVKKNMFVNHPYRWATIGSMEHLDAATLEEFQAFNKKFYSPNNAVLVVAGQIDI
                     AQTKAWIQKYFGPIPRGEKITKKTFVEEPITKTIRATYEDPNIQKPMVVAAYRTPSMK
                     TRDARVLDMISTILSDGKSSRLYKKIVDDKKMALQIGAFNYSQEDYGQYILYGMPQGE
                     FTSEDLIKEIDEEIVKLQTDLISENDYQKLQNIYENNYVNSNSSVEGIAENLASYYLL
                     YGDVNLINTENAMYRSITREEIRDVARKYLNPNQRLILDYVPAKDKAQN"
BASE COUNT         9012 a         4574 c         4554 g        10137 t
ORIGIN      
        1 ttttttcttt tgccatttga atacttttcc ccaaaagtag ttaaaatgaa aacagctatc
       61 aagttgacag ctgttttctt tataaattct attttttttt tacgaaccac acatttcgca
      121 atcatcaccc attgttccgg ctgcgtttct tgctctttct accatagcgg caaaatcttc
      181 ggcagtcatt tcgccatttt ctactggcgc aacaacttct acagcaactg cttcgggttt
      241 ttcagcgatt ggttctgctt ttttatcgtt gtttaatgta aatttaatag catcaaccgc
      301 tgcttttgtt ctcaagtaat acattcctgt ttttaatcct gacttccaag cgtagaaatg
      361 cattgatgtt aattttgcat aattggcatc ttgcataaac aaatttaacg attgcgattg
      421 gtcaataaaa tatcctctat ggcgtgacat atcaatgata tctttcatag acatttccca
      481 aacagttttg tacaattctt tcaaatcatt aggaatttgg tcgatatttt gaaccgaacc
      541 gttatgacgc atgatttctt gctttaacgt ttcgttccac aaacctcttt caactaaatc
      601 gtataataaa tgcttgttaa ctacaataaa ttctcccgac aatacacgac gtgtgtaaat
      661 attggaagta taaggttcaa aagcttcgtt atttccaaga atttgtgagg ttgaagcagt
      721 tggcattggt gctaataata atgagtttct aacaccgtct ttcatcactt ctttacgtaa
      781 gtttttccaa tcccaacgac cagataagtc ttcgtctttt aatccccaaa gattgtattg
      841 gaattcacct tgtgaaattg gagaaccttc aaaggtagaa tatggtcctt cttcttttgc
      901 taattccata gaagcagtta ccgctgcaaa atagatggtt tcaaaaattt cttggttcaa
      961 ttgttttgct tcatcgcttg taaatggcat tctcatcatg ataaatgcat ccgccaaacc
     1021 ttgaacgcct aatccaactg ggcgatgacg caaattggag ttttcggctt cttttacagg
     1081 ataataattt ctatcgataa ctttattcaa gttacgagtt actcttttgg taacattata
     1141 gagtaattca tgatcaaatt ttccgttttc aacaaacatt ggtaacgaaa tggaagctag
     1201 attacacaca gcaatttcat ctggcgatgt gtattccata atttcggtac acaaatttga
     1261 agaacgaatg gttcctaaat tcttttggtt cgattttcta tttgctgcat ctttatacaa
     1321 catgtatggc gtaccggttt ctatttgcga ttcaagaatt ttttcccaaa gttcacgagc
     1381 acgaatagtt tttcttcctt ttccttgtgc ttcatacgaa gtataaagtg cttcaaattc
     1441 atcaccatat acgttatcca atccaggaca ttcattagga cacatcaacg tccaggtagt
     1501 atcggcttca acgcgtttca tgaacaaatc agaagtccac atagcaaaga ataaatctct
     1561 agcacgcatt tcttcttttc ctgtgttttt tttcaaatct aaaaattcaa aaatatcagc
     1621 atgccatgtt tctaaataaa ttgcgaaact tcctttgcgt tttccgccac cttgatctac
     1681 gtaacgtgct gtgtcattga aaactctcaa cattggaaca attccatttg aagttccgtt
     1741 tgtaccacga atatacgaac ctgttgcacg aacattgtga attgctaagc ctattcctcc
     1801 tgcagattgt gaaattttgg ccgtttgttt taacgtatca taaattccat caatactatc
     1861 atcttgcatg gttaatagaa agcaagaaga catttgaggc tttggtgttc cagcattaaa
     1921 taatgttggt gtagcgtgcg taaagaattt tttagacatc aaatcatacg tttcgataac
     1981 agattctaaa tcatttaaat gaattccaac tgaaacacgc attaacatgt gctgaggacg
     2041 ttctacaatt tttccgttga tttttaacaa atacgaacgc tctaaggttt taaatccaaa
     2101 gtaatcatag ttaaaatctc gagtgtatat aatatgcgaa tctaaaaatt cggcattttc
     2161 ttggatgact ttatgaactt cttcagaaag tagcggtgct ttcagattgg ttcttggatt
     2221 aacataatga aacatttcgt tcatcgtttc agagaacgat ttgttggtgt ttttatgtaa
     2281 gttagaaata gcgattcgag ccgcaagttg tgcataatct ggatgagaaa ccgtcataga
     2341 cgctgctgtt tctgctgcaa gattatctaa ttcagaagtg gttacaccat catataatcc
     2401 ttcgattact ttcatggcaa cttttactgc atcaactaat tcattcaaac catagcatag
     2461 ttttttaatt ctatctgtga ttttgtcgaa cattacgggt tctttgtgcc catctctttt
     2521 tactacgtac ataaatttta gtttttgaaa atagaaatcc ctttactatt ttcgagttag
     2581 tgttttgttt gttaatccct taacgtttat ggaacttgcg ttccataagg taatctaaaa
     2641 attgaaacaa ttaaaaatca gcgtcgaaac taattttttg gtcatcgtta tcttttgaca
     2701 taacgcctgc tttttggtat tcggcaacac gtttttcaaa gaaatttgtt tttccttgta
     2761 atgaaatcat gtccataaaa tcaaatggat tagaggaatt atattctctt tcgcatccta
     2821 attctactaa caatctatcg gctacaaact ctaaatattg cgtcatcaat gtggcattca
     2881 ttccaatcaa acttactgga agcgattctg taataaattc tctttcgatg tttaaagcat
     2941 tcacgatgat ttctctaata cgttcttttg gcactttatt cactaaatga tggttgtgta
     3001 aatgcacagc aaaatcacag tgaacacctt cgtcacgaga aattaattcg ttagaaaagg
     3061 ttaaacctgg cattaaacca cgctttttca accaaaaaat ggaacaaaag gcacctgaga
     3121 aaaatattcc ctccactgct gcaaaagcga ttaatcgttc ggcaaaagag tctgattcaa
     3181 tccattttaa tgcccaatct gcttttttct taatggctgg aaaaacttct aaagcattaa
     3241 acaattcggc tttttcggct tcgtctttta cataagtatc aatcaataac gaataggttt
     3301 cactatggat attctccatc atgatttgaa aaccatagaa gaattttgcc tctggatatt
     3361 gtacttcatt tacgaaattc tcggctaaat tttcgtttac aatcccatca gatgctgcaa
     3421 aaaatgctaa aatatgtttg atgaaatact tttcgtcatg acttagttta ttattccaat
     3481 cggttaaatc ttgatgtaaa tcgatttctt ctgctgtcca aaagcttgct tccatttttt
     3541 tgtaccaatc ccaaatatcg tggtgtttta ttggaaatat aacgaaacga tttttgtttt
     3601 cttgtaaaat tggttcaatt gatgccattt tttatatttt ttttgggtaa tttgttaaat
     3661 tctgtatgtt acaaagattg gtatttgaaa tgtaaaatga aagtcaaact tatccacaaa
     3721 acggcttagt ttttaacaaa taggttttaa ttttgatgtt tttaaacaaa aattaaagta
     3781 ttgattttca aatgtttaat gataaaaaat agttgttaaa ccttgaaaac aaagggaaat
     3841 aagaaaattc tttacgctaa attttaacac ttttcatgac tttaaatttg ttttttgaga
     3901 ataaaaaaag agtcaaaaaa aacaattctg actcttagtt attttttaaa aataaaagtt
     3961 tatttaatct ctatcgatat aggttttatt accgttttta ttgatataat atttaccgcc
     4021 ttttggacct gtataaactt tttttccttt gtaagttcca gtaattttat cttttacttt
     4081 tttctcagct gtttttgagg cagccgtttc tttcatgtct tctttttctt caacttcggt
     4141 ttttttagct ttagcagtat taattttagt ttttgactta ctctcttccg cttttatttc
     4201 tttttttgca ctcgctactt tttcattagt ttctttcttt gtctttgaaa tttccttttt
     4261 agcctcggtt acttttttat cttgttcctt cttagcttta gcaaccttct tatcaaggtc
     4321 ttcttttgct ttttcagctt ttttctcggt tttcgcttta gtttttgcta ctttttcttt
     4381 agttgtttga gcaaaaatga tatttgaaaa cacaaacatc atacacatta acattacttt
     4441 tttcatgatt gtttctttta aattttaaat taaaattaaa attaaataat ttttgaatac
     4501 aaaaaacaat tatttaaaaa taagcacgca attgtacatt tccggtgtga atggtttggc
     4561 tagtttcaga ttttctgcct tgataattaa gattaatatc caaaaactcg gttagatttt
     4621 tttgtagtaa caatcgccaa gttgaatttt ttccaggctg taaaccttcc agcatttgga
     4681 aagctactgg agaattggca tcacccgtaa attgattttg gtaaaaagaa atttctccat
     4741 taatagtaaa tcgtttatca cttgcatacg aaaaagaagt tccaaatcga ctttgaagca
     4801 atttgtctaa cgttcctatc tgattttctt tgttttgata ttcataaaaa acatcccaac
     4861 tggcgttttt attgaacaaa taagaaactt ttggattgat ttgatagcct aaaacttcga
     4921 aatttctact ggcataattt tcggaagtta aggatgtttc aattgtttta gttgcaaaag
     4981 taaacaacca tgtttttcgg actaaatgat tgtattgtaa ttgatgcgaa ctgtttttac
     5041 tttcttgtga accggccgaa agcaaacttt ttattctatt ggaaataaaa gtataagttg
     5101 tagaatgtct ttgttttcct cgattgtaga ataaactgtt tctgaaactg gtgttcaatc
     5161 ctaataggtt agtgtctgat gttgaaaatg gatttaaatc aaaattgttt ccgtttcgtt
     5221 gaatttttct ttcgattaaa aaagaggttt ggttgtaaaa ataagaagcg acttttttga
     5281 gaccggtttc attttgccaa ttatttggat taatggttaa ggattgagaa aatttatttt
     5341 gatgtgtttt taaaaatatt tggttgggta aaaacactcg aacatattta gcttgatcgg
     5401 ggaatggcgc aacttcaaat tcttctaatt cttgaatacc gttgttgtta taatcgttcc
     5461 aagtgtaaac tccttgtcct ggttcgactt ctaaataggt gaattcttgt tgtggaatgg
     5521 ttccagaagt ggtttcgtaa gcggtggttg tttgtagaaa ttgattaaaa aatcggtcgt
     5581 tatacaaaat tcttgaattt aatgacggtt cattttgcct tgtattatca gtatatttta
     5641 aatctctata atttacaaag acagacaaat cacttttatc tgtttgtatt aattttgact
     5701 ttatataata ggaatttgag cggtttactt tttgtaaata tccgttttgt aaactgtcat
     5761 ttacccgatg caaatagcct aattcgacaa aaaccttggt gctatcgcca cgaccgacga
     5821 aagctccgta ctcggaaaag cgttggctta aagccgaaag ttggttggta attttattct
     5881 tttcctgatt gtcttccaaa ttaaaaactc cgcctatcca attcttttta aaattgtatc
     5941 tggcttgcga ttgatttcga gcaaaagtag aactggctat acttccgttg ctttttaaat
     6001 agctgccgtt ttgagtgatg ttccaatttt ttagtttgaa gaatccgtta atactatgtc
     6061 tgtttcccga aaaactttcg gtaaaggata ggttttcata ctgatacgtc aattttcctt
     6121 tttctggaag aacaaaatcc aaaccattga ctagtaaact ttgatttccc gaaaatgtag
     6181 tcaaattcca atcacgatta aactcaatgt tgaataatcg ctcgatggtt cggaaatctt
     6241 tttgaatgaa ttgatacttt ccaaagacat cgatgttcca tttttttgaa agaagtcgtt
     6301 gtttgaagtt taattttccc gccaatcctt tattgttatc gtcatctaga ttggaaaata
     6361 gattcaaatc attattacta acaccaactt caaaatcgat taaagttttt tcgtttggat
     6421 taaatttacc caaaaccgtc gcaatttgaa tttttgttgg cgctactaat cgaactacgg
     6481 gttcataatt tccttgtgga acgccattaa ctggttcaac atattgaaaa attttaccga
     6541 tagataccga atttgaaagt atataattac cgagattatt tccaatcaat aaaaacttaa
     6601 cattatacaa ttcgtcatca ggattgttgg aatattcaaa aacctcaaca ccgttaacag
     6661 taactttttt atataaaatt ttattttcgg aataagtgtc caaatatgcc gatggtgcat
     6721 tcatcaagtt tacatcatca ccagcatttt gtaatacttg cacttgctca gcagaaagat
     6781 tttgttgtaa cggttggttt tttacatcat tttcagaata caaataaccg cctaaactcc
     6841 attttttagc ttcgtgattg gcacctgcgt aggtcacaaa acgagtataa cttctatcgg
     6901 aatattggta ttcaatcaca atacgcattt cggaagttat gggaaacaat gatgtaaatg
     6961 taacttcacc tgcattataa tcgataacat aatcattgtt ttcgccacgt tgtttcaaaa
     7021 ttccattcac ataaactcgc tctgaacccg aaatgactaa aacatacaat tctccattgt
     7081 tacctcgtaa tttatatggt ccttgatttc cttcttgacc ggttaaggag cttttggcat
     7141 attgcccgcg aactaatgca gcagaagcaa aaatatcgtt tttgttttct tcgccaccga
     7201 atgtgaaatg tgtagaaagt ccttgtactt ttttattgaa atttagaaaa cgtgattgtc
     7261 gattttcaag aaataaatcg ccggcgcgaa tgttccaatt atcggtgaaa agttcgatga
     7321 agatttggtc aaattcatct aatttttgag aatagccacc actttgcaac gggatattgc
     7381 tgtcttgaat agaagcacga agcgagactt tatcggatat ttttcctgtg atttgcaaat
     7441 cgaggttgga ggaaacggta gtgttttgat tatttccaac ggtaacacca cgggtaatac
     7501 ttccagaagt gttcaatccg tcaaatggga tgaatttttt gacttctcga tttactttgt
     7561 ataagtttcc atcttcgttt gggacgatta cttcatcgct gtagatgctg taggttttgg
     7621 ttaaaaaatc aggatattga aggtattgaa caactaaagt gtctgaaatt ggagcgtttt
     7681 tattgaaaat taatttgccc gacggaaaat ctattttata aaacgtgctg tctattggct
     7741 ttccgttttt ggtttctagt ttgaaaaaac ttgggttgat gctgactttt tcaatttcga
     7801 tggtgtcttt tgaaatggcg attttcttgg ttttgtacaa cgaattgatt tcctgtgctg
     7861 agagctggaa gcaactaatt agtaaaaaac aatggaaaat tattttgtgc atacatatat
     7921 aaacgaaaaa aactcaagtt aagtataatt tattcctttg taaccaattg caaagttcct
     7981 ctagagattt gttttagcaa aatagttttg tccgtttcaa ccattcttgc cgctttttca
     8041 tcaaaatgtc tgatggtgta tagcgataca ttttcattgt atgacacttt aaattttttg
     8101 gataaaatgg attttaattc gttaaaattt ccaaatttat cctctacaca taccgaaaaa
     8161 ctaatggccg aattttgaat taagcttact ttgattttat attcgtgtaa taatttgaaa
     8221 atctcgctga tgttttcttc cattataaac gagaaatcga tagatgataa cgaaattaat
     8281 aattgatttt tctttaaaat aaagcaagat gtttgtggca ctaaatcggc accttttgaa
     8341 acacttgttc ctggtaatag tggatttaag aatgacttca catataacgg aatttctttt
     8401 ctttgtaatg gttgcaaggt ttttgggtga attactgtag caccataaaa cgccaattcg
     8461 atagcttcgc gatacgaaat ttgattaagc aaagtagcat tttcaaaaaa tcgtggatcg
     8521 gcattcatga ctcccggaac atctttccaa atagttacac tttcagcatt taggcaatag
     8581 gcgaaaattg ccgccgtata atcggaacct tcgcgaccta aagtggtggt gaaattattt
     8641 tcatcggaac ctaaaaaacc ttgggtaata ttaagtgctt tcttttttac gccttttgaa
     8701 atgtaacctt gtgttttatc ccaatcaaca gttgcatcac gataattatt gtctgttttt
     8761 atgaagtttc taacatcaat ccagttattt tttaatccaa catgattgaa ataatgactg
     8821 acaatggtag tagaaatgat ttctccataa ctcactactt ggtcgtaaac aaaattataa
     8881 tttggtgatt tattacttct gataaaatat tctagatctg caaaatgact gtttactgta
     8941 tagaaaacgt cgtggtcttc atcgtcaaac aaatccatta aaatttggtt atggtacttt
     9001 ttaacttctt gcaaagaaga atgtaattct ggagacttat caaaataatt tttgataaca
     9061 acttccaaag cattagttgt ttttcccatt gcagaaatta ctaaaagtgt gtcttcatga
     9121 cctacttttt ctaaaacact gtaaacattt tttattccat cagcatcttt aactgacgct
     9181 ccaccaaatt tgaaaattct catgttaatt gtagatataa gattttagac tttagattta
     9241 tttgtaagaa aattaaaaat tccttgttcg tccatttgag caattcgcca atcgtcaaaa
     9301 actttggcac ctgatttttt ataaaactcg attgctggcg tgttccagtc taaaactgcc
     9361 cattctattc ttcggacatt gtctgttttt ccttgttcaa taatttttga atatagggca
     9421 aatcctagac ctgaaccgcg cattttttct tttacaatta aatcttctag atgaatggtt
     9481 tttcctttcc aagtggaata tcgataataa tataatgcca tgcccacaat ttcgttttct
     9541 aattcggcta caaaacaatg aaataaaggg ttctcacaaa atccatcaca cactaaatcg
     9601 ttaacagtta tttcgactgc ttgtggttct ttttcaaaag tggctaactc ttgaataagt
     9661 tctaaaacag ctttcatgtc ttctttatgg gctttacgta ttatcatagg gtactatttg
     9721 aaacaaaaat acaatttata atttcttttg gaaatagatt ttttcttaga ttaatttttc
     9781 gatatttgca agaacaacta caacgatttc gcaatggaag aacgcaatag aaccttaggt
     9841 gagtttatca tcgaaaacca aaacgctttt caatattcat cgggagaatt atctcgaatt
     9901 atcaattcaa tccgattggc ggcgaaagtc gtaaattaca aagtaaacaa agcaggttta
     9961 gtagatattg ttggtgcagc tggtgatcaa aatattcaag gtgaagacca acaaaaatta
    10021 gatgtttatg ccaatgaagt ctttattcaa acacttataa atagagagat tgtatgcgga
    10081 attgcatcag aagagaatga tgatttcatt accgttgcag gttcagataa aagtcatagc
    10141 aacaaatatg tagttttaat ggatccttta gatggttcgt caaatattga tgtgaacgtt
    10201 tcggttggta caattttttc ggtttacaga cgtgttaccc ctgtaggaac tccagtttca
    10261 agtaaggatt ttttacaacc cggaacacag caagttgcag caggttatgt aatttacggc
    10321 acttcaacga tgattgttta tactactggt catggtgtta atggttttac tctaaatcca
    10381 gcaattggaa cattttatct ttcgcatcca aacatgcaat tttctaaaga tggcaaaata
    10441 tattctataa acgaaggaaa ctacgttcat tttccacaag gcgtgaaaga ctatttgaaa
    10501 tattgtcaat ttgaagaaga cgacagacct tatacgtcaa gatatatcgg aagtttggtc
    10561 tcggatattc acaggaatat gataaaaggc ggaatttata tctatccaac aagttccaaa
    10621 gcaccaaatg gaaaattacg cctattatat gaatgtaacc caatggcatt tattgccgaa
    10681 caagctggcg gaaaagcatc ggatggtttt ggaagaatta tggaaatcga acctaaagaa
    10741 ttacaccaac gcgttccgtt tttttgtgga agttataata tggtagaaaa ggcagaagaa
    10801 tttatggcaa aatataagta aaaaaaattc ccgatgttct cgggaatttt tttgttattt
    10861 attcatattt gtcatttcgt aaacaaaagt atccaaatct acattcatta ctagtaatga
    10921 caacgcttta tcaacaatat aaggaacatt tcctggtgta aagcctagtg ccaatgcaaa
    10981 cttagtcaaa atttcttttt gtcttggacc aagtatatga tcagcataga ccattctagt
    11041 taaatcatac aaacgctcca aacgttgtga atgtaaataa ggcggattga tagggtattt
    11101 taaaggattc tctaaaattt cctcatattc tgctggagaa atttctaaac gaatagctaa
    11161 tttatccaaa aactcttttt cttctttgct caaatctcca tcggcaatgg caactctaac
    11221 aatggcagaa aaatgacctt tatttctatt tttaaattcg ctatcaaata aatctgaaaa
    11281 tgacataata attatggttt tatttctgca aatataatcc aatttactat ttgatggaaa
    11341 cgaaatacaa ttgtttccaa taattttaac tacatttttt aatacttttt aaaataaatc
    11401 gtaagtttac cttacctaaa ctacttgtta tctacttatg tctgattttt ggatatattt
    11461 caaccatggc ttgaaacata ttttatcgtt ttcagcctac gatcatgtgt tatttttact
    11521 agcacttgtg gcttcctatg agtttaaaga ctggaaacga ttactgattt tagtcaccac
    11581 ttttacactt ggtcatactt tagcattatt cctttctgtt ttcaatataa taacggttaa
    11641 atcggaatgg gttgcttttt taattccgat aacgatattg attgtcgcag tttataatat
    11701 agttgctgct ggaaaatcat tcaaaaaaaa cacgattact tttatagcat tagttacagc
    11761 ttttttcgga atcattcatg gtttagggtt ttcaagttac tttaatactt ttgtttctgg
    11821 aaaacctagc gacaaattag caccgctttt agaatttgct ttgggtatcg aagccgccca
    11881 aatcatagta attattttgg cattactttt agcctatgtc gttcaaacac ttttaaaatt
    11941 ctctaaacgc gattggattt taatcgtttc ggcttttatt cttggtgttg ttgttccaat
    12001 gattgtacac aataaaattt tgagcaacta aaatttcctt aaattaactt aaaacttttt
    12061 taagagccaa tcctttcggt atctttgtcc tataatcatc actatctgta ttattttcaa
    12121 cacatgaaag aaaaaaaaca gcacaaatac gacgttgcct atctaagaat cgcttccgaa
    12181 tggagcaaat tatcctattg caaacgcaaa caagtaggtg ccatcatcgt aaaaaataga
    12241 atgattattt ctgacggtta taacggaaca ccaagtggtt ttgaaaattg ctgcgaagat
    12301 gatgaagggt taaccaaatg gtatgtgctt cacgccgaag ccaatgctat ttcaaaatta
    12361 gcacaaagtt cacaatcgag tgaagatgct accttatata ttactctttc gccatgtaaa
    12421 gaatgtagca aactcatcca tcaatcagga attaaaagag ttgtttttca aaatggctat
    12481 cgcgatgatt cgggtttaga atttttaaga aaagcaggcg ttatcgtaga acaaattgaa
    12541 agcttagaag tttagcaaaa tgaaatcgaa agaaaaatat ttaccaattt tacttttttc
    12601 ctgcatcgga tttgggataa tgatcggtgg tatgctcaat tttcctactc aaaaactttc
    12661 ttccaaaaac gccagtcaag tcaaactaaa ccgtttgata aactttattg acaatgatta
    12721 tgtggatgaa gtaaacaccg attcgatagt agatttaacc gttaacagca ttttagaaaa
    12781 gctcgatcca cattctattt atattccgcc gagcatccaa actagtgaag cagaaattat
    12841 gaaaggtgat tttgtgggga ttggcgttaa tttttacatg cacaacgata ccgttgcggt
    12901 agttagtcca ttgccaaatg gtcctgccga aatagctggt ataaaagctg gtgatagaat
    12961 tttatttgca gatagaacca aactttttgg cagaaatctt ccttccgata ccttatacaa
    13021 taaattaaaa ggcgatgatg gttctgacgt tttgttgact atttttagaa aaagcaccaa
    13081 tgaaaaatta aaattcaacg taattcgcag aacaattccc ataaaaagtg tggacattgc
    13141 tactcttatc aacccaacta ctggatatat caaaatcagt cgttttgccg aaactactta
    13201 tgacgaattc cacaaaggat tgactggttt gaaaaacaaa ggtatttcga cattaatcat
    13261 cgatttaaga aacaacggcg gtggctactt agaacgagcc gttgatattg cagatgattt
    13321 gctaaaagac aacgaattaa tcgtttttac caaaaataaa aaaggcactg tcaacaagac
    13381 atttgctacc gaaaaaggta tttttgaaaa cggaaaagta tttattttaa tcgatgaaaa
    13441 ctcagcttct gccagtgaaa ttcttgccgg agctattcaa gataatgacc gcggaatcat
    13501 tgttggtcgc cgttcttttg gaaaaggatt ggtgcaacga gaaatggatt ttgaagacgg
    13561 ttctgccgtt agattaacca ctgcgcgcta ttatacacca agcggtcgct ctattcagaa
    13621 agcttatact aaaggagaag gcgaaaaata cagtcacgaa tttgacactc gttttgaaag
    13681 cggcgaactg tatgcaaaag acaaaatcaa aattgccgat tccttaaagt ttaaaaccaa
    13741 aaaaggaaga attgtttatg gcggcggcgg aattgttccc gatatttttg tgcctttgga
    13801 agttaaaaaa ggagaagaag gtgttgctta tcttctaaat actggagttg ttggtcattt
    13861 tgtttttgaa caattagaca tcagtagaaa aaaatacaaa ttgatgactt atactcaatt
    13921 tatagcttct gttaatgaag atacaacatt ggttatcaaa tttgaaaaat acctttccaa
    13981 atcaggtttt gaagttgatt taagctctaa taaagatatt gttaaaaaaa atatcgttgc
    14041 cgagtttgtc cgtcagctct ttagtgatga acaatcgttt caaattttat tgaaagaaga
    14101 cccgatgata aaagcggttt taaagtaaat aaaactactc tcttttatca acttcaaaca
    14161 atttagccac agaaacatct aaaactttag gtgtaaattt tacatttgca atatttgtat
    14221 gtatcattcc tctttcaatt cctatatcaa aagccaattg cgcttgagaa ataccttttt
    14281 cttttctaaa cttttttaat taaacaccaa attgcttaag aaccaggagt ccgagataag
    14341 tttattgaaa aaagtgataa ataaagttct tttgagtaat aacctttatc cataattaat
    14401 ttgccgaaaa gtttgttgat aatcttttta atttataaag gatttgtgtc ctgaatgttt
    14461 gctagagtat gcataaagtt aataattctg ctacaatcat tattaagata taaattgaaa
    14521 acaaagaaca aacctgagaa acaaagacct ctttgaacaa tttcaatgaa tgtcttgcat
    14581 tggtaaatcc ttctactttt ataaatacga agtggtgtat aatcaataaa acggacactt
    14641 aaataatgag caaaatcttt tgtaaccaga aataagaaaa gtacttgcaa agtaattatt
    14701 tctgatagtg ataaattgga ctttttaata cgttttttac cgttagaaat taattgattt
    14761 tcctcgcttt gaacaaaatc attacaaaaa taatcaatag aacaaaaaat atgtgtaatt
    14821 ttatattgcg aaagaataag cttattttta gttaagtata tgtttttcaa tgatttaaac
    14881 atgttaactt ttgtttttct tataagtttt ttaacccatt ttatatcgaa cacacattaa
    14941 ataattattt aaagtcaatg cctaataggt ttcaaaaact tgttaggata actagtttct
    15001 tcaaaaacaa tctaaaaaaa agcatcatac ttattgtttt gttgtcggtt tactattttt
    15061 cattgccaaa aatacttttt gaagaaccct attcaactgt aatcgaaagt agcgaaggcg
    15121 aattattagc ggctaaaatt gctcgtgacg gtcaatggcg atttcctgct aaagatagca
    15181 ttccagataa gtttaaaaaa tgtattgttt attttgaaga cgagtatttt taccatcatc
    15241 ctggctttaa tcttgttgca atgaccaaag ctattcagca aaacagaaaa gcaggaaaag
    15301 tggttcgtgg cggaagtaca ttgacacaac aagttattcg tttggcaaga aaaaataaga
    15361 aacgaactta ttttgaaaaa gctattgaaa tcattttagc aacacgttta gaatttcgtt
    15421 cttcaaagga agaaattttg aatttatacg ctgctcatgc accatttgga ggaaatgtag
    15481 ttggtttaga aatggcatca tggcgttatt ttggggtgca atcgcatcag ttatcttggg
    15541 cagaaaccgc cactttagca gttttaccta atgctccaag cttgatttat ccgggtaaaa
    15601 atcaaataaa attgcagaaa aaaagaaatg cgcttttaat gaagttattt gatgaaaaag
    15661 taattgataa acttacgtat gagctttcca ttcaagaacc attgccacaa aagccatatg
    15721 atgtgccgca aattgcccaa cacttattgc aaaaagtagc caaaaccgat gaaggaaaac
    15781 ggattaaaac cacaatttcc tatccaattc aaaaccgagc aaatcaaata gctcaatatt
    15841 attacaatca atacaaacaa aacgaagtac acaatttagc aattttagtt attgatgttc
    15901 aaaacagaaa tattataagt tatgttggaa attcgccaac ggataaagaa cataacaaag
    15961 atgttgatat tatcagtgcg ccaagaagta ccggaagtat tttgaaacct ttgttgtttg
    16021 cttctatgat tgacgatggc gaattgttgc caaatacttt ggttgcagat attccaacac
    16081 aaatttcggg ttatacacca cagaatttta ataattcttt tgacggtgca gttccagcgc
    16141 aacgtgcatt ggcgcgatca ctaaatattc cagcggtttt aatgttgcaa gaacatggcg
    16201 ttaataaatt ctatgaatta ctgcagcaat tcaagcttcg aaatattaat aaattgcccg
    16261 atcattatgg cttgtcatta attcttggtg gtgccgaaag caatctttgg gatttatgtc
    16321 gaacttattc taatctaaca ggaacgttga attatttcaa tcaatcgaat ggaaaatata
    16381 gaagtaatga atttgtagaa ttgaattatc gaaatggttt caagcaagat tttggcacat
    16441 caggctttca aaaaaatatt cttagtgcag gttcaattta tttaacgtat aacgccatga
    16501 aagaagtaaa cagacctgaa ggcgatgaag catggaaatt ttatgattcg gcgttgcaaa
    16561 ttgcttggaa aacaggaact agttttggta atcgtgatgc atgggcaatc ggaaccaatt
    16621 caagatatgt tgttggcgtt tgggtaggca atgccaatgg tgaaggaaga ccagaattaa
    16681 ctggagttac gaatgctgca ccaattttgt ttgacatttt taatgtatta ccacgaaaaa
    16741 attggtttca accaccgcta aacgatttag aaacggttga agtatgcaaa caaagtggtt
    16801 atttagctca agaagattgc ccaaaagtta aacaactgat aacaaaaatt ggaaaaaaaa
    16861 cagccatttg tccgtatcat aaatgggtac atttggacaa atcagaacaa tttagagtaa
    16921 atagtgattg tgaatcagta gaaaatatag tcaataaaaa atggtttatt cttccgccag
    16981 taatggaatg gtattataaa tcacaacata tagattataa aactttgcca ccatatcgtt
    17041 cagattgtca acaataccag caagcatcga tggattttat ttatccgaaa cccaatagta
    17101 aaatttattt ggccaaagat ttttatggaa aaattcaacc ggttgtactc aaaatagcac
    17161 attctaatag agaatcgaaa ttattttggt cgttagacaa tcaattttta ggcgaaacac
    17221 aaacgtttca tgaaatgcca ataaaaccag aaacaggaat acattacatc accgttacgg
    17281 atgaatttgg caatgaaatc aaacgaaaaa tcgaaataat aaacgagtaa tttttgttaa
    17341 gttctgttgt ttagagaact agttcagtac tttggagaat tagtttcctt tttattaaaa
    17401 atacaggcac aaaaaaaccg ccaatctttc gaaaagcggt ttttattttt ctatagtttc
    17461 tttccttctt cgttgaagaa atgatattct agatacgtgt aagcgtcacg tggtatgatt
    17521 tttacccatt ttttgtgttc aaaaagccat tttgaacgca atgatggaaa acctttttta
    17581 aggtatgcag ccacaaaagg atgaacatta agtttaatct ttttatggtc tttaataatt
    17641 ttttcaaggt cagaagtgat gttgtcaatt atcgaaatcg gtgcttccac atcgctcgat
    17701 agattgtttg gattttcttc tcttgtttca atatttactt ctggtcttac acgttgtctt
    17761 gtaatttgga ctaatccaaa tttacttggc ggtaagattt tatgtttggc tttatcgtca
    17821 tccatttcta atttcaagaa atcatacaac acttttcgat tgtctggatt ttgcatatca
    17881 ataaaatcga caacaataat tccacccata tctcgaagtc ttaattggcg agctatttct
    17941 gctgctgcaa tcatgttgac ttccaatgct gtatcttctt ggttagaagc tttgttggaa
    18001 cgattaccac tgttcacgtc aataacgtgt agtgcttcgg tgtgctctat aattagatat
    18061 gcacctttgc tcatggaaac ggttcttcca aatgatgttt ttatttgtct ctcaatatga
    18121 tatttttcaa ataatggaac atcattggat tgataaagtt taactatttt ttgtttctca
    18181 ggagctattt cggctaaata gtccttggtt tcttggtaca actccttgtc atcggttaag
    18241 atacttgtaa aagtatcatt aaatacatct ctaagaatgg cagaagcttt gttaagttcg
    18301 cctaaaactt ttgatggatg atgagcagtt ggtaattttt tacacattgc agtccatcta
    18361 ttcattaatg actgcaaatc tttttctaat tcggctatac ttttgccttc ggctactgtt
    18421 ctcacaataa ccccaaatcc tttttgtttt atagattgta ctagtttttt taaacggtct
    18481 ttttcttctt tagattctat tttttgagaa acagaaactc tatccgaaaa aggtaccaac
    18541 actacatagc gaccagcaaa ggaaagttct gcacttatcc ttgggccttt tgtagaaata
    18601 ggttccttaa ccacttgaac taaaaccgat tgattggcac tgattaaatc agttattgcg
    18661 ccatttttat caatctcttt ttcaaattgg aaatttttta gggagaaatc ttttagttta
    18721 cctgcgctta caagttttat gaacttcata tacgagttga aacttggacc taaatcatga
    18781 taatgtaaaa aggcatcctt ttcgtagcca acatttacaa aagcagcatt caatccagca
    18841 acgggttttc ttattttggc aataaatata tcgcctacta agaagttgct agtgtcttct
    18901 tgtttatgta attcaattag ttttccatct tttaataagg caaaatctac agcatcttca
    18961 ctagatctaa tgattagttc tttattcacg ttgtaaattt taatctgcaa ctttgttgtc
    19021 agaaggttga tgatagtgga atagttttag aaggaaaaat aattcttcca acttccaact
    19081 tccagcttcc aacttccagc ttccagcttt ttcacagatg gattaaacat ttttaacagg
    19141 tattattacc cgagaggaaa attaaatcca aatttcaaaa tccaatataa atgctgttag
    19201 gaattttaga attttttgat ttggtttttt tgaaattttc caaatttcaa tgaacgttaa
    19261 aaagaaaaaa gtagtttaga actacttttt ctttttgtgt cggttagctc tcgcacgttt
    19321 tttacgcttg tgagtagcta ccttatgtct ttttcttttt ttaccacttg gcatagaata
    19381 ttctttaggt gattaataaa taagtttata ctggaatttc agtattagtt tttacagact
    19441 ctacaaatat tttagcaggt ttaaatgctg gaatattatg tgcaggaatt ttgattgtag
    19501 tgttcttaga aatgtttctt ccagtttttt cagctcttgt tttaataata aagctaccaa
    19561 aacctcttaa gtaaacatta tcaccagttt ctaaagaagt ttttacttct tccataaatg
    19621 cttcaataac tgcttgaacg tcttgttttt caagaccttt tctttctgaa atctttgcta
    19681 cgatatctgc tttcgtcatt ttctttcgta tttaaatatt ataagtaatt ttttgaggtt
    19741 gcaaatatat gaattaaaaa aacaatattt caagcataat ctattaaaat ttaatcagat
    19801 aaagaattat ttttgcagac tactttttat atatgaattt ttctaatgct ttaatagttt
    19861 ggtacttaca aaacaaacgc aatttacctt ggcgaaacac gctcaaccca tacctaattt
    19921 ggctatcgga aataatacta caacaaacgc gagttgctca aggattgccc tattttttaa
    19981 gttttgcaga acgttttcca actgttttcg atttagcaaa tgctgatgaa gaagaagtct
    20041 taaaactttg gcaaggttta ggctattatt ctagagcaag aaatcttcat aaaacggcaa
    20101 aaactattgt ttttgattta aatggtaaat ttcctaaaaa ttacaatgag ttattaaaac
    20161 taaaaggagt tggcgaatat actgctgctg ctattgcttc ttttgcttac aatgaaaatg
    20221 ttccagttgt tgatggaaat gtatttagag tattagcccg atattttgat attgaaactg
    20281 atattgcatc ttctaatgct aaaaaagaat ttagagcatt ggcaagtaaa cttttgccca
    20341 atggaaaagc taaccaattc aatcaagcca ttatggaatt tggagctttg caatgtgttc
    20401 ctaaaaatcc taattgtgaa atttgtgttt tgaacaatag ttgtcttgct ttaaaaaata
    20461 aaaaagtaac tcaattgcct gttaaactta aaaaaacaaa gattcaaaaa agagtgttta
    20521 attatttgat ttttagagat gaagataaaa acactttgtt gcaaaaaaga acacaaaaag
    20581 gaatttggca caatttatac gaatttccat tgatagaaac ttttgaagac ataaatgatt
    20641 ttgaaacgct tgaattgatt caaaatcaat cttttattgt taatgaaatt caaaatatta
    20701 gattgataaa tccagttaaa ataacccata aactttcaca tcagcatttg tcagtttctt
    20761 tttgggaagt ttctgtggca ggaaaaatta aagacggaat tgattgtgat tcggtgttaa
    20821 aatttccttt tcccattgtg atttataatt ttatttcaaa aatttgggaa taagtttcaa
    20881 aaaagcagta aatttgagag tataaaaatt tcaaaaatga gtagtggtac tttaaataaa
    20941 gtgatgttga ttggtcatct tggagacgaa ataaaaatgc actattttga aggaggaaat
    21001 tgcattggta gatttccttt ggctacaaat gaggtttata ttaacaaaca aaccaacgaa
    21061 aaaattacat ctacagaatg gcacaatctt gttgttagaa ataaagctgc cgaaatatgc
    21121 gaaaaatact tatccaaagg cgataaaata tatgtagaag gaagaataaa atcgcgtcaa
    21181 tggcaggcag aagatggcac atttaaatat actaccgaaa ttcaagttac agaatttatt
    21241 tttttgaata ccaaaaaaga tattgacgga acaaaaaata ttcagaatac tgattcaaaa
    21301 aacactactt ttgagcttcc aaaaccagag aatgatttgc caaatttgga tgctgatatg
    21361 ccaacttaaa aacttgttta actaatattt cgctttttgg acacagaacc cagtcttaat
    21421 tttatagaaa ccgttgatac taatattatt ttaggtagta tcggcgtttt tattttattg
    21481 ttcttttctg caatggtttc tgcagcagaa gtagcattgt tttcgctaac accaaatgat
    21541 ttgaataaac tgaatgaaga aaaccctaga aaaggtgatt tgctttcaaa gttaattcaa
    21601 aaacccaaaa aacttttagc tactttactt ttagccaata attttttaaa tataggtgtt
    21661 gtaattttgt tctcaatggt tggcgatacg ctattttccg gaatttcttc accaatacta
    21721 aagtttgtag ttgaagtcat tctagttacc tttctgattt tgctttttgg tgaagtttta
    21781 ccaaaggttt atgccagtag aaacaatgta aaattttcgg tagcacttag tactcctata
    21841 tttatattag acaatttatt gtcgccaata agtttgccaa tgcgaaacat tacagttttt
    21901 ttgcatgcta aatttgggaa tcaaaaaagc agtttgtctg tagatcaatt atcgcaggcg
    21961 ttagaattaa cttctgatga agatactacc caagaagaac aaaaaatttt ggaaggaatt
    22021 gtgtcttttg gaaatacaga tacaaaacag gtaatgagtc caagaattga tgtttttggc
    22081 ttggatattg atgagtcttt tgcggatatt tatccaaaaa ttatagaaaa aggatattct
    22141 agaattccgg tttatcaaga aaatattgac gatataaaag gtgtgctttt cgtaaaagat
    22201 ttaattccac acattcataa aaaagagttt gattggaaaa aaattattcg agaaccattt
    22261 tttgtgcctg aaaacaaaaa attagatgat ttgttaaaag attttcaatc gatgaaaagt
    22321 cacatggcaa tagtggttga tgaatatggt ggaacatcag gattggtttc tttagaagat
    22381 attattgaag aaattgttgg tgatatatct gatgaatttg atgatgaaaa cattaatttt
    22441 tctcaaattg atgatagaaa ttaccttttt gaaggaaaga taacactcaa agatttctac
    22501 agaattatcg atgttgatga agatttattc gaaagtaaaa aaggcgaagc agaaactttg
    22561 gcaggtttta ttctcgaaaa tcttggaaat tttccaaaaa aaggacaaaa aatacatttt
    22621 tcaaatttgt tttttaccat agaaactgtt gataaaagga gaattaaaca aattaaagta
    22681 actcttgaat aatgaaaatc aatttcaaac atattttagt tttagtaatt atggttttgt
    22741 tttttagctg taaagacgat gttttgccaa aaccagccag tcaactccga ttggattatc
    22801 ctttggcgga atatgttttt tactctgatg cttgtccttt tacttttgat gtaaacgatg
    22861 aagttgatgt gaaagaagat aaaaattgcg gttttagcat tcattatcca aaaatgaaag
    22921 ctactattta tttgacatac aaaccagttt ctaacaatat agacaaatta cttcgagatg
    22981 cacaaaaatt gacttttgaa cacgtgatta aggctgatgc aattcaagaa cagccgttta
    23041 tcaacgctga tagtaaagta tatggtatgt tttatagagt taacggaaat gcggcaacaa
    23101 atgctcagtt ttatgttacc gatagtgtga agcattttgt aactggttcg gtttatttct
    23161 atgccaaacc taattttgac tcgattatgc cagcgacaag ctatgttcgc gatgatatgc
    23221 aacatttaat ggaatcgttg caatggaaga attgatttaa gcaaaaaatc cagcaattgc
    23281 tggatttttt tatgagtcat ttgtttttga attatacttt tatcttaaca tcagaaactt
    23341 tatatgtttt tccgtcagct gttttttcaa caatttcttg gattttctct ggtgaaatta
    23401 tagcagcatc aaaattaacg gttgcttcct ttttgtcaaa atcaactttt gcatcttgaa
    23461 caccttctgt tgaagatagc tttttctcaa ttgtttttgc acaacctatt gcacaactca
    23521 tgccatcaat agtaaatgat gccgtttcgg gtttagtagc aattgttttt ttgctttcta
    23581 tggttacaga gtctgaagcg ttagtttgtg attcagcttt tgagtttgtt tctttacaac
    23641 cagttaaaaa taatgtacat aatgctaatg taaagatgga acttgtaaat ttcataaaat
    23701 atatttttag tattagtatt tgtaaatttt aggacagtac aaaaataatt aaaaattaaa
    23761 gttattattc ttaaaataat tacaaaattt gaatcctaaa aaaaacattc atgaaatcac
    23821 ttcaaataaa atgggtttat ttaatcgttt tagcactcat ttggggaagt tcatttattc
    23881 tgattaaaaa aggattagtt gctttgtcac catttcaatt gggttcatta cgaatcatct
    23941 tttcttctat ttttttgtta attcttggat ttaaatcctt gccaaccata ccacttcata
    24001 aatggaaatt tattgcatta actgcttttt tgggaacatt ttttcctgct tttttgtttg
    24061 cttttgctca aacacaaatt agtagttctg tgagttctat cttgaattca ttaacacctt
    24121 taaacacact actactaggt ggtttatttt ttggggttgc ttttaaaaga acgcaaataa
    24181 taggtgtgtt tattggtttg tgtggcacac tgttgttgat tttaaatggt gcagttcatc
    24241 atccaaatca aaattacttt tatactattt tggttattat cgcttctatt tgttatgcca
    24301 cgaatgtaaa tttgattaaa aagcatttat ccgatattaa gccattggca ataactacag
    24361 gaaacttttt tgtgttatta attccagcaa gtattgtttt gtattttacc aatttttgtt
    24421 caattataag tgaagtggaa gttcaaaatg ctacagtata tatcctaatt ttaggaattc
    24481 taggaactgg tattgcgaat attattttct ttaagttgat tcaaatttcg tcgccagttt
    24541 ttgctacttc ggtaacttat ttaattccaa ttgttgcctt tttttggggt ttgttagatg
    24601 gtgaattttt aacgccagtg caagtaattg gagcttttat tattttggtt ggagtttaca
    24661 tgtcgagtaa aaaataaaaa accgtttcaa aattaattga aacggtttct tctataaata
    24721 aaaaagtatt tcttatttga aatcagcgtc agttacacct tcgttaactt tcacatcgct
    24781 cataataaag tttaattcaa atccttgatt gattgttatt gcatgaggaa ttttaactcc
    24841 tttaacgtct ttataatcac tgtaagcagt agttacagtc atttttttgt cgccttgctc
    24901 ttcaagttta gattctgaaa gctttaatcc tgattttaca tcgtaatata aagttgtttt
    24961 tccgttttta attacataag catcagttcc attcattggt tcaatgccat caagaactaa
    25021 atttgggtta ttaattaaaa ctaattcgtc aaaagttgct gcggtttctt tttcttcagc
    25081 aaattcttca gcagtcattt cttttcgttg accttgagct gaggaataac cacctttttc
    25141 agttacaact tgtctgttca attcgcccat tccatccatt gaaataacca ataaagatct
    25201 tcctttgttg tcttttttta tcaccatttt gattggtgct ggagcttgag gaattgttgc
    25261 agaacctgtt atagatactg tttttactgc cataacagct ttttcaccac caattgcttt
    25321 gatatagttt tctataaccg attttgcagt tacgccagct ggtgcagctt ttttcatagc
    25381 aggtttttct gtcggagcac catatttatc aaaatagaaa attggaattt ttaatttttc
    25441 taaacctggt aatacatcag tagcttttcc aactacaata attctaagat tatcagctaa
    25501 catgtattta ttggcaactc tcatgatgtc ttctggagtt acagcattaa tgtttttgat
    25561 atagttttca taaaaatctg ctggcaaacc ttcggtctga atgtttagtg cataacgagc
    25621 cactgtagct ggtttttcaa cttgcataac aaagcgtcca atgtaacccg cctttacatt
    25681 ttttagaaca gtttcgtcaa ctttttcagt tctgattttt ttcaattctt tcaaagcttc
    25741 aactactgca ctatcggtaa ctgtatttct aacttgtgtg ctggcagcaa atttactatc
    25801 gccatatttg tcatttccta catatgatct agcgccatac gtccaaccat gtgcttctct
    25861 caagttcatg tttaagtaac tgttaaagtc accaccatat acttgattgg caacaattac
    25921 aggaaaataa tcaggatctg aatatttcaa acttacagta tttactaagg caatttctga
    25981 ttgtaccgca tttggcatat ccacaaaatt gatttgagta tattgaacgt ttttaggatt
    26041 tgaataggtt aatcttggag cagttgcttt tacccatgac ccaaaaaggc tttcaaccat
    26101 ttttttaatt tcctttggtt taacatcacc aacaactact agataagcat tctctggaac
    26161 aaaataggtt ctatagttgt ttttaacatc agctaaagaa acattattaa tggtttcttc
    26221 ggataaatat tctccagaag gatgattttt cccataggct aaaacatctt gaactctatt
    26281 tgctgctgcc gaaacacttt tttcttgtgt ttttaatcct tcgattaatt tgtctctatc
    26341 tttatcaaat tcttcttgag taaaatttgg cattaaagca ccttccgcca ttaattctaa
    26401 tattcttttc gaatattttg ataaaccatt agcagaagca ccagaggcat agaaattgat
    26461 gtttgcgccc aaaaaatcta tctcttcatt gaacttgtct tttggtgttt ttgttgatcc
    26521 atttcctaaa agactgcttg ttaactctga aactcctttt ttgttacctt ctgcataggg
    26581 tgcattgtca attgttaaac tataagacac tcttggtagt ttgtggtctt caactactag
    26641 cacttttagt ccgtttggca atgaaaaagt ttctggtttc ttaatattaa ttttaggaga
    26701 aggtccaggt tttggttgag ttctgtcttg tgcttgcata gtaattgtta aaaacaagct
    26761 tgataatata attgctattt ttttcataat aatttttggt cttagttttg agctttgtct
    26821 ttagcaggta cgtaatccaa aatcaatctt tgatttgggt ttaaatattt tctagctaca
    26881 tctctaattt cttctctagt gatggaacga tacattgcat tttctgtatt gattaaattt
    26941 acatcaccat ataataggta ataagaggca agattttcgg caataccttc cacgcttgaa
    27001 ttactgttta cataattatt ttcatagatg ttttgtagtt tttgataatc attttcagaa
    27061 atcaaatcag tttgaagttt tacaatttct tcatcaattt ctttgattag atcttcagaa
    27121 gtaaattcac cttgaggcat tccgtaaaga atgtattgac cataatcttc ttgactgtaa
    27181 ttaaatgccc caatttgtaa agccattttt ttgtcgtcaa caattttttt gtacaaacga
    27241 gagcttttac catcacttaa aatagttgaa atcatgtcta aaactcttgc atcacgagtt
    27301 ttcatcgatg gagttctgta tgcagcaaca accattggtt tttggatgtt agggtcttca
    27361 taagtagctc ttatcgtttt tgtgattggt tcttcaacaa acgttttttt agttattttt
    27421 tcacctcttg gaattggacc aaaatatttt tgaatccaag cttttgtttg cgcaatgtca
    27481 atttgaccag ctactacaag aactgcgtta ttcggagagt agaatttttt gttaaatgct
    27541 tgaaattctt ccaaagttgc tgcatcaaga tgttccatag aaccaatagt agcccatcgg
    27601 taaggatgat ttacaaacat gtttttctta acctgcgcaa tcaagttccc ataaggttga
    27661 ttgtcaacac gtaatctttt ttcttcttta acaacttcgt tttgtgtgtc caccccaatt
    27721 tggttaataa ccggatgcat taaacgttct gattccatcc ataaaccaac ctcaagactg
    27781 tttgaaggga aaacttcaaa ataataggtt ctgtcgtcag aggtgttggc attatttgtt
    27841 ccaccattag cagtaacaat tttaaaccat tcaccacgac ctatattttt tgtaccttca
    27901 aataataaat gttcaaaaaa gtgcgcaaaa cctgttctct ctggattttc gtctttcgaa
    27961 cctacgtgat acataacaga agtaataaca actggagccg atgggtcgtt atgtaaaata
    28021 acgtgcattc cattgtccaa gtcgtactct tcaaaagcaa ccttttgtgc agaagtaatt
    28081 ccacttagca taagcattgt acttaaagca attaatgatt ttttcataaa gaaatgtaat
    28141 tttttcgttg atagttatat tagtaacaca acttttatgt ttgttacatc aaaaatacct
    28201 ttttttttct ttttttgaat ataaatgagt gtttaaatca ttaatttagt attattgcta
    28261 ataatttttc tcaagat
//
LOCUS       DAOOBH010000018        27599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_2277_length_27599_cov_5.648889, whole genome shotgun sequence.
ACCESSION   DAOOBH010000018 DAOOBH010000000
VERSION     DAOOBH010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 27599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27599
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2277_length_27599_cov_5.648889"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..883)
                     /locus_tag="PK218_03665"
     CDS             complement(29..883)
                     /locus_tag="PK218_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPW97639.1"
                     /translation="MKAKITVHIYAKSSKANKSGQLPIYFRLTVNGERFEFSTKKFID
                     KSKWSSELSKMKGHSEEARSLNNYLDLIKSKIFDIQMELLHKNKDLSLENFKARLTGT
                     HERERMIIPIYQSHNDKIKDLIGNGYAYGTLERFKISLKHLQEFILWKYNVSDININK
                     IDYAFVTEFEFYLRSVKKCNNNTAVKYVRNFRKIIKICLDNDWLDKDPTTRYEGKMKE
                     VERDFLTEEELLKIYNKKIFSERLQLVRDIFIFSCYTGLAYIDVKGLKKDHIGIGIDG
                     EKWIFKNL"
     gene            complement(1156..1230)
                     /locus_tag="PK218_03670"
     tRNA            complement(1156..1230)
                     /locus_tag="PK218_03670"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1193..1195),aa:Val,seq:tac)
     gene            complement(1300..2520)
                     /locus_tag="PK218_03675"
     CDS             complement(1300..2520)
                     /locus_tag="PK218_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPW97640.1"
                     /translation="MNYQEVLEWMFHQLPMYQTQGASAYKNDLNNIILLANHLGNPEK
                     KIKCVHVAGTNGKGSTSHLLASILQEAGFKVGLYTSPHLKEYRERIKINGKEITKNFI
                     VDFINTHKLFFEDNKLSFFEMSVALAFDYFEKQKTDINIIEVGMGGRLDATNIITPLL
                     SVITNIGLDHTQFLGNNLEAIAVEKAGIIKKNIPIIIGEYLDETKKVFLNKALENKSE
                     IFFVSDLILENYPSKLLGDYQEKNKKTVLQTIKALNQKYNYAITEETIKKGFLNVIKN
                     TGLKGRWQILKQTPFIVCDTAHNYDGFKITINQILKHNFNTLHIVLGFVNDKDINTIL
                     EILPKKAKYYFCKPNNSRGLNQDVLQAKAAVFGLKGEVYNSVTKAYEQAVKSATNADF
                     IYIGGSTFVVAEII"
     gene            complement(2533..3318)
                     /locus_tag="PK218_03680"
     CDS             complement(2533..3318)
                     /locus_tag="PK218_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPW97641.1"
                     /translation="MKYLETPEEKKSFTITSVIFVILFLLFFFFGLTYMDPPPENGIA
                     INFGTSNVGQGEVQPEKPVQSAPKPEVAQPQKAVEEDIATQDNTDAPVIKKAEVKKPI
                     KEVKEPVKPTPTPSKTTTSALESLINGPKTDGVKAGHGNDGVPGDKGDPNGDRYANSF
                     YGNGTGNGSGNGTSWGLNGRKLSTPAKEVQDCNESGTVVVQVTVNRNGNVIDAKYSKG
                     TTNTNPCLVNPALSAARKFKWQPDSDAPATQIGFIVINFKLGE"
     gene            complement(3327..3719)
                     /locus_tag="PK218_03685"
     CDS             complement(3327..3719)
                     /locus_tag="PK218_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPW97642.1"
                     /translation="MNLRGRNKVSPEFNMSSMTDIVFLLLIFFMLTSTMVTTNALDLV
                     LPKAKGKTDSNKSISISIDKDLKFYLDKTPIEEASVEAQLLAAIGGDKEKPVILRAEE
                     GVPIEKAVYVMDIANRNQLKIVLAVRPK"
     gene            complement(3721..4425)
                     /locus_tag="PK218_03690"
     CDS             complement(3721..4425)
                     /locus_tag="PK218_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPW97643.1"
                     /translation="MSLFLQADTLSVAKDALTDAAPVEQTLSIWELITSGGLAGQIIM
                     VALFVMLFFAIYLYLERLMAVSAASKIDNNFMMQIKDHISNGRLDAAKMLCASTNSPV
                     ARLIQKGISRIGNRLEDINTAIENAGKLEVYKLEKNVSMLATISGAGPMTGFLGTVVG
                     MIMAFHKMASGGGQIEVGALAEGIYTAMTTTVVGLIVGLIAYMGYNHLVVRTDKVVNQ
                     MELNAVEFLDLLNERK"
     gene            complement(4531..5760)
                     /locus_tag="PK218_03695"
     CDS             complement(4531..5760)
                     /locus_tag="PK218_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HPW97644.1"
                     /translation="MKDLLQQFEDKQPEIIFNWKDSETEAEGWTVINSLRGGAAGGGT
                     RMRKGLDMNEVLSLAKTMEVKFSVSGPAIGGAKSGINFDPNDPRKKGVLQRWYKAVSP
                     LLKSYYGTGGDLNVDEIHEVIPFTEECGVWHPQEGVFNGHFKPTEADKINRIGQLRQG
                     VIKVIENPKFSPDVLRKYTIADMITGFGVAEAAKHYYKTYGESIVGKKAVVQGFGNVG
                     SAAAFYLADMGAKVVGIIDRDGGVINEEGFSFDQIKQFFLTKDGNKLVSDNMIPFDEI
                     NERIWNIKSEIFAPCAASRLVTKNQIDSMIASGLEVISCGANVPFADKEIFFGPIMEA
                     TDAKVSLIPDFISNCGMARVFAYFMEKKVQMTDEAIFEDTSNIIKKAIQNIHTKNSSK
                     TNLSATAFEIALQQLVQ"
     gene            5939..6616
                     /locus_tag="PK218_03700"
     CDS             5939..6616
                     /locus_tag="PK218_03700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW97645.1"
                     /translation="MKNIIITGTSRGIGYELALQFANAGHQVLAISRKIPQELLENQN
                     ISCLSIDLSKENDLVKIEDFLESTWKKVDIIIHNAGSLVNKPFAQTTQSDFENVYKVN
                     VFGVANLTRVSLPYLQNGSHVVSISSMGGIQGSMKFAGLSAYSSSKGAVITLSELLAE
                     EYKEQGIAFNVLALGAVNTEMLQEAFPGYEAPTSAKEMADYIFNFALTGNKFHNGKVI
                     QVSSSTP"
     gene            6620..7222
                     /locus_tag="PK218_03705"
     CDS             6620..7222
                     /locus_tag="PK218_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HPW97646.1"
                     /translation="MSEVLAKYIPEHAVETLFELIKMNSVHLKIVNERQTRHGDYRKG
                     LNGKHEITVNANLNKYRFLITLVHEIAHLVAFEKYGRLIKPHGNEWKITFQRLMLPFI
                     RPEIFPHSVLPLVANHFKNPTASSDTDARLAFALKQFDERKSDIHFIHEVPSGSFFRI
                     KNGRIFQKKGLRVKRYECLEVKSGKLYLFNANAEVEIIPG"
     gene            7322..8383
                     /locus_tag="PK218_03710"
     CDS             7322..8383
                     /locus_tag="PK218_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPW97647.1"
                     /translation="MNKNYYAILMAGGVGSRFWPVSTTEFPKQFHDMLGSGTTLIQKT
                     FSRLSKLIPSENILILTNEKYNDLVLEQLPLVKPEQVLLEPAMRNTAPCILYASLKIQ
                     KQNPNAVMVVAPSDHWIEDEDEFARNLQKCFDFCQDNDALMTLGIQPTFPNTGFGYIE
                     FDKSDTNPIKKVNQFREKPDYETAKSFLEQGNFLWNGGIFIWSAKLITAAFEKFQPQM
                     NSLFQKGIESYNTNKEQDFITINYANAENVSIDYAIMENAKNVYVLPATFDWNDLGTW
                     GQLHEKIDKDENNNGVINAKVVIENASNNIIRSDANKLIVVDGLHDYIIVDKEGILLI
                     YPKSKEQDIKRITKLANEL"
     gene            8454..11891
                     /locus_tag="PK218_03715"
     CDS             8454..11891
                     /locus_tag="PK218_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPW97648.1"
                     /translation="MKKSLKVLLITVICYINSYSQIDNFTLSVTPTNETCTANGKLTF
                     SVSNTLPGSTVIYTIYKLPNLVTPITVTSVSPFTGLNSGTYRVVATQTLGNQSSTKQQ
                     DVVITDQRTNLTYQVNGVDEVCGLDGKLTITILTGNVFNYEIISGPIIRPLQTSNVFN
                     NLSAGVYQIRVFDVCGEGVVQTYQLFHKNTALNLTLKPVSMAGCNSVSVGFGIAPLTE
                     DGIVKYPLQVTTLVTSQTGVITTNNDVVTSGTNFQAIVPYYSQQPYNYTITVVDGCGI
                     TYTLNGVVNGVSPTAIYSLESQDCDYKKVTLYNVSSVTLVSAPATFSTSLPINYSSQI
                     VNSRVVITNLTAGTYVLNVVNVCGIQQTITFEVVIDSPLDPYYTLFNRTCVSSSVLIF
                     GVQQLIMITAPATYNVALPHDYSSLINPANYAGFSNLPIGVYQFSTIDMCGQPKPLTI
                     VINPISESPIATVLEGCDEGLGSLKITGQITTITLTSAPASYSNFAIPHNFTSSLISN
                     SSALVIGLLPPGTYVFKTTNACNVEYTLTAQVFGLIENSTASIIPNCGSYNVNVIEDS
                     NSSENTYWLQKLNTTTNDWVHPDTNVVYPNGTLPIAGNSIELIGNGTVFNLTALGQFR
                     VLKAYKTYMDNTIASINCFRVLAEFEFTGQPSIKEVLSISCGATFEVLVTAEGVAPLQ
                     YRITLKNGQPFVIQNGTSNYFANLEPAIYNFQIEDACGNIVNRIFEIINPNPITITAS
                     TVVCNGDTLTMTVPTFSFLEYQWWKDNNISTILSTTNELTIASFNTALNNGTYHVRIV
                     YPNNPSSCLNQELTYTIALNVSSPNAGQGSTNTYCGSQGTIDLFTLLQGSYQTDGVWT
                     ETTNSGMLTNNLWNSTSLTFGTYLFNYRVTGSCNQFANSVVSVTLFSIPNAPIATADA
                     IVCESSDIHLYASTVTNANYFWTGPNGYTSTQQNPLLTTVSQATNGTYTVYVTQNNCE
                     SDEDSVAIVVNPLPDFTLNQECVGKDYVVTAVFDENNTSGILSWIGPDAFSSNQNPII
                     ITRAKTGIYSLTVTNPSGCTTTKDIDVIRTFCEIPNVITPNNDGSNEELNLIGFDVNK
                     IEIFNRWGRLVYEKNNYSNEWHGQNQKGEKLPDGTYFYLITLETNEVKNGWIFVSGN"
     gene            complement(11888..12652)
                     /locus_tag="PK218_03720"
     CDS             complement(11888..12652)
                     /locus_tag="PK218_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW97649.1"
                     /translation="MIEVKNIEKSFGDTKVLKGITTTFETGKTNLIIGQSGSGKTVLL
                     KSLLGIHTPEVGTISFDGRIYSELTDDEKRDLRTEIGMVFQGSALFDSMTVEENVGFP
                     LKMFSKKTPTEIKERVNLVIARVNLIDSNHKKPSELSGGMQKRVAIARAIVNNPKYLF
                     CDEPNSGLDPNTAIVIDNLIKEITEEYNITTVINTHDMNSVMEIGEKIIFLKNGLLAW
                     EGSKKNIFKTDNEAVTSFVYSSNLFKKIRKAQNKEV"
     gene            complement(12652..13398)
                     /locus_tag="PK218_03725"
     CDS             complement(12652..13398)
                     /locus_tag="PK218_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW97650.1"
                     /translation="MMLIRYLSQIGKYFLMLKEVFNKPTKWSVMRGLIFKEIDDLIID
                     SLGIVAFISFFVGGVVAIQTALNLTNPLIPKYLIGFATRQSVILEFAPTFISIIMAGK
                     MGSFITSSIGTMRVTEQIDALEVMGVNSLNYLVFPKIIALLLYPFLIGISMFLGILGG
                     WMAGVYGGFTSSTDFTQGLQTEFIPFHIAYAFIKTLLFALILATIPSFHGYYMKGGAL
                     EVGKASTVSFVWTSVVIILTNYILTQLLLT"
     gene            complement(13468..14316)
                     /locus_tag="PK218_03730"
     CDS             complement(13468..14316)
                     /locus_tag="PK218_03730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPW97651.1"
                     /translation="MNYYLVIPTYNEERFIALTLQSLVAQTVLPAKIVVVNDGSTDST
                     ESIVQSFCDKYPFITLVNKTSDAIHLPGSKVIQAFQKGLETLDNQYDFIVKADADLIF
                     PANYFEMISKHFLSDSNIGMVGGFAYIEKNGEWILENLTDKDHIRGAFKAYRKETFKQ
                     IGGLKPAMGWDTVDELLCKFYNWKVVTDETLKVKHLKPTGANYNKAARYKQGEAFYSL
                     GYGFLITAIASLKLALRKNKPLLFFDYINGFKKAKTAGKSLLVTEEQAQFIRNYRWKK
                     MKEKLF"
     gene            complement(14384..14911)
                     /locus_tag="PK218_03735"
     CDS             complement(14384..14911)
                     /locus_tag="PK218_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPW97652.1"
                     /translation="MYEKTYPSKRFNITLDFLKKHINPSETIFDLGVPNPFSKIMEDN
                     GYTVINTKGEDLDNDQSALQTENYEIFTAFEIFEHLLNPYTVLQNVKCNKVLISIPMR
                     LWFQSAYKSKTDKWDRHYHEFEDWQLDWLLEKTGFKIIDRVQFTHPVKKIGFRPLLRF
                     FTPRYYLVYAERNPK"
     gene            complement(15011..15406)
                     /locus_tag="PK218_03740"
     CDS             complement(15011..15406)
                     /locus_tag="PK218_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97653.1"
                     /translation="MKKIFFTLLVFGMLSACNKDDNKSNTSIVGNWKLIEVLEDPGDG
                     SGTFMSVDSEKTITFNSDGTLTSNGDLCDMTTSTLNPTSGTYDATNATITSDACVQTT
                     LGYNYVQNGTVLIINYPCFEPCQAKYQKQ"
     gene            complement(15483..15830)
                     /locus_tag="PK218_03745"
     CDS             complement(15483..15830)
                     /locus_tag="PK218_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HPW97654.1"
                     /translation="MDNTKIYKMAFASVYPHYINKAEKKGRTKEEVHTIIQWLTGYDE
                     KALQEQLDNKNDFETFFGQAQLHPNASKITGVICGYRIEEIEEPLMKKIRYLDKLIDE
                     LAKGKKMEKILRT"
     gene            complement(16026..17084)
                     /locus_tag="PK218_03750"
     CDS             complement(16026..17084)
                     /locus_tag="PK218_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPW97655.1"
                     /translation="MNIKITGSGSYIPTEVVSNIDFAKHVFLNDDGTPFPHPNDVIAQ
                     KFLEITGIKERRYVTDDLVTSDIATIAAKIAIDDAKVDAESLDYIIFAHNFGDVKKNA
                     IQGDMLPSLAAKVKHNLRIKNPKCVAYDMLFGCPGWVESVIQAQAFIKAGMAKKCLVI
                     GAETLSRVVDMHDRDSMIYSDGAGATIIEATNEEGGILAHESASFTYDEANYLYFGNS
                     FNTFNDPDVRYIKMYGRKIYEFALSEVPKAMASCLEKSGVDINQVKKILIHQANEKMD
                     EAIVQRFYRLYKQTVPEGIMPMSIHKLGNSSVATVPTLYDLLLKNNVENHSLGKGDIV
                     LFASVGAGMNVNSIVYKV"
     gene            complement(17208..17591)
                     /locus_tag="PK218_03755"
     CDS             complement(17208..17591)
                     /locus_tag="PK218_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HPW97656.1"
                     /translation="MNDIETREDLLYIMRTFYDKLLADETINFYFTKVTDVHHHLEEH
                     FELLATFWEQSLFMKGGYSNNMFQIHKDINAKYSFTKEHFETWLKHLYATIDNDFKGK
                     NAEQMKTNALSMATVMQIKIVHQNA"
     gene            complement(17719..20562)
                     /gene="gcvP"
                     /locus_tag="PK218_03760"
     CDS             complement(17719..20562)
                     /gene="gcvP"
                     /locus_tag="PK218_03760"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HPW97657.1"
                     /translation="MKTDAFALRHLGPRENDLPEMLKTVGVETLEQLIYETVPDNIRL
                     HKELQLDAPMTEYEYANHIRILGRTNKVFKSYIGLGYHPTIVPPAIQRNIFENPGWYT
                     AYTPYQAEIAQGRLEAILNFQTTVIELTGMEIANASLLDESTAAAEAMALLFDVRSRD
                     QKKNNVCKFFVSEEILPQTLSVLQTRSTPIGVELVVGNHETFDFSSDYFGAILQYPGK
                     YGQVNDYTSFIAKANENEIKVAVAADILSLVKLTPPGEMGAAVVVGTTQRFGIPMGYG
                     GPHAAFFATKEEYKRSMPGRIIGVTIDTNGNRALRMALQTREQHIKREKATSNICTAQ
                     VLLSVMAGMFAVYHGPKGLRYIADKVHASAVTLANALNKLGVYQTNTSFFDTIVVKAD
                     AKKVRAIAEKNEINFFYIDDETISIALNETILFKDINEVIAVFAEALGKEAFEVNEYA
                     LENNYPMHLNRKTEFLQYDVFNSYDSETSLMRYIKKLERKDLSLNHSMISLGSCTMKL
                     NAAAEMLPLSMPYWNTIHPFAPLDQVQGYIGMLKKLEQQLNVITGFAGTTLQPNSGAQ
                     GEYAGLMAIRAYHIAEGNSHRNVCLIPASAHGTNPASAAMAGMKIIVTKTMENGNIDV
                     EDLRTKAIENAENLSCLMVTYPSTHGVFESAIKEITQIIHDNGGLVYMDGANMNAQVG
                     LTNPATIGADVCHLNLHKTFAIPHGGGGPGVGPICVNEKLVPFLPSNPLVKVGGDQAI
                     TAISGAPYGSALVCLISYGYICMLGAEGITNATKYAILNANYMKAKLEEHYPILYSGE
                     MGRAAHEMILDCRSFKAKGIEVTDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESEDLA
                     EINRFCDAMIAIRQEIEAAAIEEPNNVLKNSPHTLAMVTSDTWTFPYTREKAAYPLDY
                     VAENKFWPSVRRVDETYGDRNLVCSCAPIEAYM"
     gene            20862..24236
                     /locus_tag="PK218_03765"
     CDS             20862..24236
                     /locus_tag="PK218_03765"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPW97658.1"
                     /translation="MKKITLTLLFFFGMVATYGQTLVLNEVMTSNLSVIADDDGDYND
                     WIEIYYNGATAINLEGYALSDDATLPMKWVFPEYWIEPGDHLLIWCSDKNRTDINNDL
                     HTNFKISSTGEVIYFNRPNGTLQDSFLAVILPENISYGRQTDGAITKVYFQQSTPGAA
                     NNTPGFSQVLNAPSINVPSGFFTSSFPVTITYPDPNVTIVYTLDGSDPVLTNLAGTNY
                     NYKNKYIELAGQTAGAQLQNSYKSFTYSSPLTIVDRSSIANDLAKMSSTFQNVPSYIP
                     TFNVFKGTVLKVRAFKNGAIPSPIITKTYFVTPQGSARYTLPVVSLSITESKFFEYND
                     GIFVAGKDFDDWRAANPTVDADFSSVNENEENANYYRISDNEEQKGHLSYFVGGQEVL
                     NQDIGIEVNGGNSRNFQSKALRLIARSEYGNDKMSYPFFANENYNSYKRLLLRNSGQD
                     FRSTMFRDGLFHTLVEKLNTETKSYQPTIVFLNGEYWGILNMRERYDKQFFNQKYNIA
                     DGELDYLKNDLTPVEGDGNHYAAMETYLQNNSLLNTANYNYIKTQLDPENMRDYYISN
                     IYAQSTDWPGWNTIFWRKKTAAYEPNAPFGQDGRWRTGINDMDDTLGEYPEGSNHNTL
                     AFATATGGPDYPNPEWSTLILRRLLENNEFKLNFINRFADLLNTYFLPSRVNAMIDIF
                     KNKINPEIAEHQARWKPFYDVETWNDYSIQSIRDFANTRPNNQRAHIRGKFGISGDIN
                     ATLNVDDVAHGFIKMNTVEINSSTPGVAANPYPWTGIYFKNIPVTMKAIALPGFVFSH
                     WSGASTSTSAEITLTTAADFSVTAHFVPAGSGGEEVPIYYWVFDSSLENNTPLSSVNT
                     TYNVNVLPQGVLNYQSCLSGYPFDASNANWRKASMERRNNPTDVNYIPEVNNNLTFAA
                     SNMRGIQVKQPFQNNGQENKLIFSSSTAGYHDIKVSFAAVNELAADGLVVDYSIAAGS
                     GTWITTGLAASSLPLTSSFQLFTIDFSSITAVNNNPNFKFRIRFSGTNLTEDLGNRVT
                     FNNISIKGKQDNLSVVEADSKLEFVMYPNPTTGVLNLVYSYQDVNYAIFSIDGKLIQS
                     GKLDNPQINVSSIQHGIYLLQLSADGKTETKKFIKN"
     gene            24389..>27599
                     /locus_tag="PK218_03770"
     CDS             24389..>27599
                     /locus_tag="PK218_03770"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPW97659.1"
                     /translation="MIKKILFFVIFFTVFFQENTFSQALVINEVVTSNSTTIADEDNS
                     YQDWIELYNNSASAINLSGYGITDDSTNLFKWVFPNVTINPGGYMLVWASDKNRTNPA
                     NPLHTNFKISSSGETITLTNASSVIVDTAPATAMQADESLGRLPNGTGGFVLIQTPTP
                     NALNVNGTPIATLDPPVFSQNSGFYTAGFNLNITTNEPGATIIYTLDGSEPDENNLSG
                     TTYNYKNQYPKIPGQAFGTFLFNTFQSFQYSASIPINDRSSQPNKISAISTTYDFTPP
                     YFPNGPIYKGTIVRAKVVKPGAVSSEVISKNYFITPAGSSKYTLPVLSFSFNENELYD
                     YEDGIAVAGIDFDNWRTANPTEIPDREIGNFERRGAANERKANMHYFVNGLEKINQDV
                     GIRIRGAYSRLYPSKSWNIYAHSEYGDGNLSYNFFADEPYTSYERLSAKSGGSDFYNT
                     MFRDALNHQLVKDLKMETESFQPMVVFMNGEYWGLLNMREKYDDNYFKQVYNIDNVDL
                     LENQGAIQEGDAVDYNNMYAYIQNNSFTNEANYTYIQTRMDTDNFIDYFITNIFTQNT
                     DWPGNNIIYWRKRTAQYEPNAPYGHDGRWRWALHDNDSTLDDASYNSLHNATSTVDVG
                     PNPAWSTLILRKLLANTTFKNDFINRFADLMNTNFLSSRIIAKIDEISAIIAPEMNDQ
                     YFRWKAPVDNGDWQYHLEEEREFAEDRPAYQRNHIRSKFGITNNINANLNVSNYDHGY
                     IKISTINIQDGTTGIIGNPYPWTGIYFADIPIKLKANARPGYLFSHWEGFSTSTNEEI
                     TITTNQSFSITAVFIPEGFAVETQEPIYFWLFDGAIPNDTPLTSLNSTFEIPVEGVLE
                     YQSCLTGYPFVNGNPNWRKASMERKNSPTNINYIPEANNNIPFASAGMRGIQIRQPFQ
                     DNGLENTIQFNLSTKDYKDIKFGFAAKNDNAADAIIIDYSITAGTPTWITTGLASSSL
                     PLSSTFELFEVDFSSIIAVNNNANFKIRLRFTGSNMTVDNGDEVVFNNFSLKGVQIPL
                     TYVTPNVYTIDTPISDLIPNLTASADSFAISPAVPNGLTF"
BASE COUNT         8819 a         4614 c         4670 g         9496 t
ORIGIN      
        1 gattcgtaag gcatctgaaa cctttaaatt aaaggttttt gaaaatccat ttttcgccat
       61 cgataccgat accaatatgg tcttttttca atcctttcac atcaatgtaa gctaaacctg
      121 tgtagcatga aaaaatgaaa atgtctctta ccaattgtag tctttctgaa aatattttct
      181 tgttatagat tttcaataat tcctcttcgg ttaaaaagtc tctttctact tctttcattt
      241 tgccttcata tcgagtagtt gggtctttat ccaaccaatc attatctagg catatcttga
      301 taatttttct gaagtttcta acatatttga ctgccgtatt gttgttgcat ttttttacac
      361 ttctcaaata aaattcaaat tctgttacaa atgcatagtc gattttattt atatttatat
      421 cacttacgtt gtatttccat aagataaact cctgaaggtg ttttaatgag attttaaagc
      481 gttctagagt gccataagcg tatccatttc caataaggtc tttaatttta tcattatgac
      541 tttgataaat cggaatgatc attcgctcac gttcatgagt tccagttaat ctagctttaa
      601 aattctctaa tgataaatct ttatttttat gaagtaattc catttgaata tcaaaaatct
      661 tggatttgat taaatcgaga tagttattta gtgacctggc ttcctctgaa tgtcctttca
      721 tttttgatag ttccgaagac cattttgatt tatcaataaa ttttttggta ctgaattcaa
      781 agcgctcacc atttaccgtt agtctgaaat aaataggtaa ttgaccgctt ttgttagctt
      841 ttgaagattt tgcataaata tgcacagtga tttttgcttt catggtggtg accttttaat
      901 tagttttcaa tttaattttt aaccttttag caaacaagat gtacatattt tgaaccagta
      961 ttaaactggt tgttagtaca gtgtttacag tacttgagtg aatttagcga gacccaaaat
     1021 tgtgaaaatt tttcgggtct cgattttatc tctttcgcaa caagattgaa tgaataattt
     1081 ggtacatgcc caaaagcaaa aagccgataa atcctaagat ttatcggctt ttgtaaccat
     1141 ttgtttttaa atgtgtgggc gatgagggat tcgaaccccc gaccctctcg gtgtaaacga
     1201 gatgctctga accaactgag ctaatcgccc gacttgtgct tgattgcgag tgcaaatata
     1261 agctagtttt ttaattctac aaatttttat tgaaaaaaat taaattattt ctgcgactac
     1321 aaaagtgctt ccaccaatat agataaaatc tgcgtttgtg gcagatttga cggcttgttc
     1381 ataagctttt gttacagaat tatatacttc gccttttagt ccaaaaacag ctgcttttgc
     1441 ttgcaataca tcttggttta accctcgaga attatttggt ttgcaaaaat aatattttgc
     1501 tttttttggt aaaatctcca gtattgtatt gatgtcttta tcatttacaa agcctagtac
     1561 gatgtgaagt gtgttgaaat tatgttttag gatttgattg attgttattt tgaatccatc
     1621 ataattgtgg gcagtgtcgc aaacaatgaa tggtgtttgc tttaaaattt gccaccttcc
     1681 ttttaaacct gtgtttttaa taacgtttaa aaatcctttt tttatggttt cttctgttat
     1741 tgcataattg tacttctggt ttaaagcttt tatggtttga agtactgtct ttttgttttt
     1801 ttcttggtaa tcacctaaca atttggaagg atagttttct aaaattaaat cagaaacaaa
     1861 aaagatttcg gatttatttt ctaacgcttt atttaggaat acttttttgg tttcgtctag
     1921 gtattcgcca attattattg gaatgttttt tttaattatt cctgcttttt caacagcaat
     1981 ggcttctaaa ttattcccta agaactgtgt atggtctagt cctatgtttg tgattacaga
     2041 tagtaaaggt gtaatgatgt ttgtagcatc taaccttccg cccatgccta cttctataat
     2101 gttgatgtcg gttttttgtt tttcgaaata atcaaaagct aaagcaaccg acatttcgaa
     2161 aaaacttagt ttgttatctt caaaaaaaag tttgtgtgtg tttataaaat cgactataaa
     2221 atttttggtg atttcttttc cgttaatttt tatacgttct cggtactctt ttaaatgtgg
     2281 agatgtgtat aatccgactt taaagcctgc ttcttgaaga attgatgcta gtagatgtga
     2341 tgttgagcct tttccgttgg tacctgctac atgaacgcat ttgatttttt tttcgggatt
     2401 tcctagatgg tttgcgagta gaatgatgtt gtttaaatca tttttgtacg ccgatgctcc
     2461 ttgggtttga tacattggca actgatgaaa catccattct aaaacttctt gatagttcat
     2521 ccgtaaaaga gtttattctc ccaatttgaa attgattaca atgaaaccaa tttgagttgc
     2581 tggtgcatcg ctatctggct gccatttgaa ttttcgagca gcagaaagtg ctgggttgac
     2641 caaacatggg tttgtgtttg tggttccttt agaatatttg gcatcaatga cattaccatt
     2701 tcggttaacg gtaacttgca ccactactgt tcctgattcg ttacagtctt gtacttcttt
     2761 tgcaggagta cttaattttc tgccgtttaa tccccaactt gttccatttc ctgagccgtt
     2821 tcctgttcca tttccgtaaa aactattggc ataacggtcg ccatttggat cacctttgtc
     2881 accaggaaca ccgtcatttc catgtcctgc ttttacacca tccgtttttg gcccgttaat
     2941 taaactttcc aaagcacttg tggttgtttt ggaaggagtt ggcgttggtt ttacaggttc
     3001 ttttacttct ttgataggtt tttttacttc agcttttttt attactggag catcggtatt
     3061 gtcttgtgtg gcaatgtctt cttcaactgc tttttgtggt tgtgctactt caggttttgg
     3121 agcagattgg actggttttt cgggttgtac ttctccttga ccaacgttgg aagtaccgaa
     3181 attgatagca attccatttt cgggtggcgg atccatatag gttaatccga agaaaaagaa
     3241 caacaaaaac aatatcacaa aaataacaga tgtgatagta aatgattttt tttcttctgg
     3301 agtttctaaa tatttcatag tataaattat ttaggtcgaa ctgctaaaac tatttttaat
     3361 tggtttctat tggcaatatc cataacgtaa actgcttttt cgataggcac gccttcttcg
     3421 gctctaagaa ttacaggttt ttctttatcg ccaccaattg ctgccaataa ttgcgcttct
     3481 actgatgctt cttcaattgg cgttttgtct aaataaaact tcaagtcttt atcaatacta
     3541 atagaaatac tcttgttgct gtctgttttt ccttttgctt ttggcaaaac taaatccaaa
     3601 gcattggtgg taaccattgt agaagttaac atgaagaata tcaacaaaag gaatacaata
     3661 tccgtcattg aagacatgtt aaactctggg ctaactttat ttcttcctct taaattcatc
     3721 ttattttctt tcgtttagta agtctaaaaa ctccaccgca ttcaactcca tttgatttac
     3781 tactttatcg gttctaacca ccaagtgatt gtatcccata taagcaatta atccgacaat
     3841 aagaccaaca acagttgtgg tcattgctgt atagatacct tctgctaatg cgccaacttc
     3901 aatttgtccg ccaccactag ccattttgtg aaatgccata atcataccca caaccgtccc
     3961 taggaaacct gtcattgggc ctgctccaga aatagtagct agcatactta cgtttttctc
     4021 taatttatat acttctaatt ttccggcatt ttcaatagct gtattaatgt cttcaagacg
     4081 atttccgatt ctagaaattc ctttttgaat caatcttgcc actggagaat tggttgatgc
     4141 acacaacatt ttggctgcat ctagtcttcc atttgagata tgatctttaa tctgcatcat
     4201 aaagttatta tcgatttttg aagcagcact aactgccatt aatctttcta aataaagata
     4261 gatagcaaaa aataacatta caaaaagggc aaccattatt atttgacctg caagtccacc
     4321 gctggttatt aattcccaga ttgataatgt ttgttctact ggcgcagcat ctgttaaagc
     4381 atctttggct actgataaag tatccgcttg aagaaataaa ctcataatat tgttattatt
     4441 taattttttt aaaaataata cttgttataa gacctctgaa attaatttca aatagtaata
     4501 cacatagtta ttaacgaaaa aaagttgttt ttattgtact aattgttgca atgctatttc
     4561 aaaagctgtt gcactaagat tcgttttgct gctgtttttt gtatgaatgt tttgaattgc
     4621 ctttttaata atgttagaag tatcttcaaa tatagcttca tccgtcattt gcactttctt
     4681 ttccatgaaa taggcaaaaa cccttgccat tccacaatta gaaataaaat ctggaatcaa
     4741 acttacttta gcgtctgttg cttccattat tggcccgaag aaaatttctt tatcggcaaa
     4801 aggaacattt gccccacatg aaatcacttc taatcctgaa gctatcatac tgtcaatttg
     4861 gttttttgtt accaatctag aagcagcaca tggagcaaaa atctccgatt ttatattcca
     4921 aatacgttcg ttgatttcat caaatggaat catgttgtcg gacaccaatt tatttccgtc
     4981 tttggttaaa aagaattgtt ttatttgatc aaaagaaaat ccttcttcgt tgatgactcc
     5041 accatcacga tcaataatcc caactacttt tgcacccata tcggctaaat agaatgctgc
     5101 tgctgaacct acatttccaa aaccttgtac tactgctttt tttccaacga tgctttcgcc
     5161 ataggtttta taataatgtt ttgctgcttc tgctactcca aaaccagtaa tcatatcggc
     5221 aatagtatat tttcgtaata catctggaga aaatttaggg ttttcgatta cctttattac
     5281 tccttggcgc aattgaccaa ttcgattgat tttgtcagct tcggtaggtt tgaaatgacc
     5341 gttaaaaaca ccttcttgtg gatgccaaac gccacattct tctgtaaaag gaatcacttc
     5401 atggatttca tctacattta aatcgccgcc agttccataa taacttttta gtaatggaga
     5461 aactgctttg taccaacgtt gtaaaacgcc tttctttcta gggtcgtttg ggtcaaaatt
     5521 aattcctgat tttgcaccac caatagctgg tcctgatacc gaaaacttca cttccatcgt
     5581 tttggctaaa gacaacactt cattcatgtc aagtcctttt cgcatgcgtg ttccgccacc
     5641 agcagctcca cctctcaagg aattaatcac tgtccatcct tcggcttctg tttcactatc
     5701 tttccagtta aatattattt ctggttgttt atcttcaaat tgttgtaata aatctttcat
     5761 ggttactttt taagttgtgc aaatataaga atatgttttt gttttacttt ttaatactat
     5821 tatagatatg ttaaagagtg ctaagaataa ttgagtcaac atgttttagg cagttcaaat
     5881 aaaaggcttg ttattttatt tgaatttttt tattcctatt tttacaaaaa ataagctttt
     5941 gaaaaatatt atcatcactg gaacttcacg aggcatagga tacgaattgg cattacagtt
     6001 tgcaaatgct ggacatcaag ttttagctat ttctagaaaa attccgcaag agttgcttga
     6061 aaatcaaaat atttcgtgcc ttagtattga tttgtctaaa gaaaatgatt tggtaaaaat
     6121 tgaagatttt cttgaatcaa catggaaaaa agttgacatt attatccata atgcaggtag
     6181 tttggtaaac aaaccttttg ctcaaactac acagtcagat tttgaaaatg tttataaagt
     6241 aaatgtgttt ggggttgcca atctgactag agtttccttg ccttatcttc aaaacggaag
     6301 tcatgtggtt tctattagtt ccatgggcgg cattcaaggc agtatgaaat ttgctggact
     6361 ttcggcttat tcttctagta aaggtgctgt gattacgctt tcggaattat tggctgaaga
     6421 atataaagaa caaggtattg cttttaatgt gcttgcactt ggggcagtta ataccgaaat
     6481 gttgcaagaa gcatttccag gttatgaagc accaacatct gctaaagaaa tggctgatta
     6541 tatttttaat tttgctttaa cgggaaataa atttcataac ggaaaagtaa ttcaagtttc
     6601 atcctctaca ccttaagatt tgagtgaagt tttagcaaaa tatatacccg aacacgccgt
     6661 cgaaactctt tttgaattaa tcaaaatgaa ttcggttcat ttaaaaattg ttaatgagcg
     6721 tcaaacgcgc catggtgatt atcgaaaagg attaaatggt aaacatgaaa ttactgtaaa
     6781 tgctaatctg aataaatatc gatttctgat tacattggtt catgaaattg ctcatttagt
     6841 agcttttgag aaatatggac gtttaataaa accccatggg aatgaatgga agattacatt
     6901 tcaaagactg atgttgcctt ttattagacc tgagattttt ccgcattctg tgttgccttt
     6961 ggttgccaat cattttaaaa acccaacggc tagtagtgat accgatgcac gattggcttt
     7021 tgctttgaaa caatttgatg agcgaaaatc agatattcat tttatccatg aagtgccaag
     7081 tggtagtttt tttagaataa aaaatggaag gatttttcaa aagaaagggt tacgagtaaa
     7141 acgatacgaa tgtttggaag tgaaatcagg aaagctatat ttgtttaatg ctaatgcgga
     7201 agtagaaatt attccgggat aatatgatta agagaagacc taaaatacaa attttataat
     7261 aaatacattt gcatccatca taagaacaca tacgtgtagg tatgaaaaaa aaacttaaac
     7321 tatgaataaa aattattacg caatattaat ggctggtggt gttggttcac gattttggcc
     7381 agtttctaca actgaatttc caaaacaatt tcacgatatg ttgggttcag gaactacatt
     7441 gatacaaaaa acttttagtc gtttgtcaaa actaattcct tcagaaaata tattgatttt
     7501 aaccaatgag aaatacaatg atttggtttt agaacaattg cctttggtga aaccagaaca
     7561 agtgttgctg gaaccagcta tgcgaaacac cgcaccatgt attttatatg cttctttaaa
     7621 aattcaaaaa caaaatccta atgccgttat ggttgttgct ccaagtgacc attggataga
     7681 agatgaagat gaatttgcaa gaaatttgca aaagtgtttt gatttttgtc aagataacga
     7741 tgctttgatg actttaggta ttcaacccac gtttccaaac actggttttg gatatattga
     7801 gtttgataaa tcagatacaa atccgattaa aaaagtaaac caatttagag aaaagccaga
     7861 ttatgaaact gccaaatctt ttttagagca aggaaacttc ctttggaatg gtggtatttt
     7921 tatttggagt gctaaattaa ttactgctgc ttttgaaaaa tttcaacctc aaatgaatag
     7981 tttgtttcaa aaaggtattg aaagctacaa tacaaataaa gagcaggatt ttattactat
     8041 taattatgct aatgccgaaa atgtttctat agattatgct attatggaaa atgccaaaaa
     8101 tgtttatgta cttcctgcta cttttgattg gaatgattta ggaacttggg gtcaattaca
     8161 cgagaaaatt gataaagacg aaaacaacaa tggtgtaatt aatgcaaaag tagttattga
     8221 aaatgcctcc aataatatta tccgctccga tgctaataag ttaatcgttg ttgatggttt
     8281 gcatgattat attattgttg acaaagaagg aatattatta atttatccaa aaagtaaaga
     8341 acaagacatc aaacgaatta cgaaattagc aaatgagcta taattttttt aataaaaatt
     8401 atcagtatat tgttttttga gttactttta ttacttttaa aaagagaatt gtattgaaaa
     8461 aaagtttaaa agtactattg ataactgtca tttgctatat taattcgtat agtcaaattg
     8521 ataactttac tttatctgtt acaccaacaa atgaaacgtg tacggcaaat ggcaaactga
     8581 ctttttcggt ttcaaacact ttgcctggct cgacggtaat ttatactatc tacaagttac
     8641 caaatttagt tacgcccatt actgttacca gtgtgtctcc atttacaggg ttaaattcgg
     8701 ggacttatag agttgtcgct actcaaacat tagggaatca aagcagcaca aagcaacaag
     8761 atgttgtaat aacagatcaa cgaacaaatt taacctatca agttaatggt gtcgatgaag
     8821 tttgtggttt agatggaaaa ttaactataa cgatattaac tggaaatgta tttaattatg
     8881 agattatttc cggaccaatt attcgcccac ttcaaacttc taatgtgttt aataatcttt
     8941 cagcgggtgt atatcaaata agagtctttg atgtttgtgg cgaaggtgtt gttcaaacct
     9001 atcaattgtt tcacaaaaat acagcattga atctcacatt aaaacctgta tctatggcag
     9061 gttgcaattc ggttagtgtt ggttttggaa ttgctccttt aactgaagat ggaattgtta
     9121 aatatccgtt acaagttaca acactagtta cctctcaaac aggcgtcata acgaccaata
     9181 atgatgttgt tactagtgga acaaattttc aagctattgt gccttattat agtcagcaac
     9241 cctataatta tactattacc gtagttgacg gttgtggaat aacttatact ttaaatggtg
     9301 ttgtaaatgg agtttctccc accgctattt attccttaga aagtcaagat tgcgattata
     9361 aaaaagtaac gttatataat gtttcaagtg ttactttggt ttctgcacca gcaacatttt
     9421 caacgtcatt gccaataaat tacagctctc agattgttaa tagtagggta gttattacta
     9481 atcttactgc tggaacctat gttcttaatg ttgtcaatgt gtgcggcatt cagcaaacta
     9541 tcacttttga agtagtaatt gactctccat tagatcctta ttatacgctg tttaatagaa
     9601 cgtgtgtttc aagttctgtt ttaatttttg gagtacaaca attaataatg attacggctc
     9661 cagctactta taatgttgct ttacctcatg attattctag cttaattaat cctgccaatt
     9721 atgctgggtt tagtaatttg cctattggtg tatatcaatt ttctactatt gatatgtgtg
     9781 gacaaccaaa acctttaaca atagttatta atccaatatc agaaagcccg attgcaactg
     9841 ttttagaagg ttgtgatgaa ggtttaggct cattaaaaat aacaggacaa atcacaacaa
     9901 taacgttgac ctctgcacca gctagctatt caaattttgc aattccacat aattttactt
     9961 ctagtctaat ttctaattca agtgctttag taattggttt attgcctcct ggaacctatg
    10021 tttttaaaac tacaaatgct tgtaatgttg aatatacact tacagctcag gtgtttggac
    10081 ttatagaaaa ttctactgct tcaattattc caaattgtgg ttcatataat gtgaatgtga
    10141 ttgaagactc aaactcgagt gagaatacct attggttgca aaaactgaat acaactacaa
    10201 atgattgggt tcatcctgat acgaatgttg tttacccaaa tggaactttg ccaatagcag
    10261 gaaattctat agaattaata ggcaatggaa cagtttttaa cttgacggct cttggtcaat
    10321 ttagagtgtt aaaagcctat aaaacctata tggataacac cattgcatcc atcaattgtt
    10381 ttagagtatt agctgaattt gaatttacag gacaaccaag tataaaagaa gttttgtcaa
    10441 tttcttgcgg agcaactttt gaagtattag ttactgccga aggagttgcg ccactacaat
    10501 atagaattac attaaaaaat ggtcagcctt ttgtaattca aaatggtact tctaattatt
    10561 ttgccaatct tgaaccagca atttataatt ttcaaataga agatgcttgt ggaaatattg
    10621 tcaatcgaat ttttgaaatt ataaatccaa atccaataac gattactgct tcaacagtgg
    10681 tttgtaatgg agatacatta accatgacag ttccaacttt tagttttcta gaataccaat
    10741 ggtggaaaga caataacatt tccaccattc taagcactac aaatgaactt actattgctt
    10801 cgtttaatac tgctttaaat aacggaactt atcatgttcg catagtttat cctaacaatc
    10861 caagttcatg tcttaatcaa gaattgactt ataccattgc attaaatgtt tcgtcgccaa
    10921 atgctggtca aggttcaacc aatacttatt gtggttcgca gggaactatt gatttgttta
    10981 ctttattgca agggagttat caaacggatg gtgtttggac agaaacaacc aatagtggaa
    11041 tgttaactaa taatttgtgg aacagtacat cgcttacttt cggaacctat ttgtttaatt
    11101 atcgtgtgac tggaagttgt aaccaatttg cgaattcagt tgtttcagta actttgtttt
    11161 caataccaaa tgcacctatt gccactgccg atgcaatagt ttgtgaatct tcggacattc
    11221 atttatatgc ttcaacggta actaatgcca attatttttg gactgggcca aacggttata
    11281 cttctacgca acaaaaccct ttattaacga ctgtttcaca agctacaaat ggaacatata
    11341 ctgtttatgt aacgcaaaac aattgcgagt ctgatgaaga tagtgttgcc atagtagtta
    11401 atccattgcc tgattttaca ttaaatcaag agtgtgttgg caaagattat gtagttaccg
    11461 ctgtttttga tgagaataac acaagtggaa tattaagttg gattggacct gatgctttca
    11521 gtagtaacca aaacccaata ataataacaa gagcaaaaac gggaatttat tctttaaccg
    11581 taactaatcc ttcaggttgt acaactacca aagacattga tgtaattaga actttttgtg
    11641 aaattcctaa tgtgattaca cccaataatg atggttcaaa tgaagagcta aatttaatag
    11701 gatttgatgt caataaaata gaaatattca atcgttgggg aagattagtc tatgagaaaa
    11761 acaattattc caacgaatgg cacggacaaa atcaaaaagg ggaaaaatta ccagatggga
    11821 catatttcta cctcattact ttagaaacca atgaagtaaa aaatggatgg atttttgtta
    11881 gtggtaatta aacttcttta ttttgggctt ttctaatttt tttgaacaag tttgaagaat
    11941 atacaaatga ggtaacggct tcgttatcag ttttaaaaat gttttttttg cttccttccc
    12001 aagctaataa tccatttttt aggaagataa ttttttctcc aatttccatt accgagttca
    12061 tgtcatgagt attgataacc gtcgtgatgt tatactcttc tgttatttct ttaataagat
    12121 tatcaataac aatagccgta tttggatcta atccagagtt tggttcgtca caaaaaagat
    12181 atttcgggtt gtttacaatg gcacgtgcaa tcgctactcg tttttgcatt ccgcccgaaa
    12241 gttcagatgg ttttttatga tttgaatcaa tcaaattaac ccttgcaata actagattaa
    12301 cacgttcttt tatttctgtt ggcgtttttt ttgaaaacat ttttagcggg aaaccaacat
    12361 tttcttccac agtcatgctg tcaaacaaag cacttccttg aaaaaccatt ccaatttcgg
    12421 tacgcaaatc tcttttctca tcatcggtta gttcggaata aattcttccg tcaaaagaaa
    12481 tagtgcctac ttcgggtgta tggattccta ataaagattt tagcaaaact gtttttccag
    12541 aaccactttg accaataatc aaatttgttt ttccagtttc aaaagtggtt gtaattcctt
    12601 ttaaaacttt tgtgtcacca aatgatttct ctatattttt aacttctatc attatgtcaa
    12661 aagtaattgt gttaatatat agtttgttaa aataatcact actgatgtcc atacgaagga
    12721 aactgtactt gctttaccta cttcaagcgc accacctttc atataataac cgtgaaatga
    12781 tggaatagta gccaagatta atgcaaaaag caacgttttt ataaaggcat aggcaatgtg
    12841 aaacggaata aactcggttt gaagtccttg cgtaaaatcg gtgctactgg tgaaaccacc
    12901 ataaacccct gccatccatc cgccaagaat tcctagaaac attgaaatac caataaggaa
    12961 aggatataat aaaagtgcaa ttatttttgg gaaaaccaaa tagtttagcg agttaactcc
    13021 cataacttct aaggcatcaa tttgttctgt tacacgcatc gttccaatac ttgaggtaat
    13081 aaaggaaccc atttttcctg ccatgatgat ggagataaat gtgggtgcaa actctaagat
    13141 taccgattgt cgagtagcaa aaccaattaa gtattttggg attaacggat tggttaaatt
    13201 caaagccgtt tgaatggcta caacgccacc aacgaagaaa gaaataaacg ctacaattcc
    13261 caatgaatca ataattaaat catcaatttc tttgaaaatt aaacctctca tcacagacca
    13321 tttggtaggt ttgttgaata cttcttttag catcaaaaag tactttccaa tctgcgataa
    13381 ataacgaatg agcatcatag ttttgaaata atgtcaaaaa tagttagaag ttatgagtta
    13441 tgggttatga gttgaaagtt ttttaaccta aaaaagcttt tctttcattt tcttccaacg
    13501 ataatttcta ataaattgtg cttgttcctc ggttactaat aatgattttc cagcggtttt
    13561 tgctttttta aaaccattaa tataatcaaa aaacaagagc ggtttattct ttcgcaaggc
    13621 taattttaaa gaagcaattg ccgtaatcaa aaaaccgtag cccaaactgt aaaacgcttc
    13681 gccttgtttg tagcgtgccg ctttgttata gttggctcct gtaggtttta gatgttttac
    13741 ttttaaagtt tcgtccgtta cgactttcca attatagaat ttacaaagga gttcgtcaac
    13801 ggtatcccaa cccatagctg gttttaatcc gccaatttgt ttgaaagttt cttttctata
    13861 agctttaaat gcgccacgaa tatggtcttt gtcggttaag ttttccaaaa tccattcgcc
    13921 atttttttca atgtaagcaa aaccgccaac cattccaatg tttgaatctg atagaaaatg
    13981 tttgctaatc atttcaaaat aatttgcagg aaaaattaaa tcggcatcgg cttttactat
    14041 aaaatcatac tgattatcta aagtttctaa tcctttttga aaggcttgaa tgactttgct
    14101 tcctggcaaa tgaatggcat ccgaagtttt attgaccaat gttataaatg gatatttatc
    14161 gcaaaacgat tgaacgatac tttctgtgct atctgtagag ccatcattga ccaccacaat
    14221 tttagcaggc aaaaccgttt gcgccaccaa agattgcaat gtcaaagcaa taaatcgctc
    14281 ttcgttataa gtaggaatga caagatagta gttcataata aattccaatt ttttaaattc
    14341 caaattccat ctgaatggat tttagtttgg gatttgggat tttttatttg ggatttcttt
    14401 cagcatatac caaataatat cgcggagtaa aaaatcgcaa taatggtcga aacccaattt
    14461 tctttacggg atgtgtaaat tgaacgcgat ctataatttt gaagcctgtt ttttctaaca
    14521 accaatccaa ttgccaatct tcaaactcgt ggtaatgtct gtcccatttg tcggttttgc
    14581 ttttgtaagc cgattgaaac cataaacgca ttgggattga aattaaaacc ttgttgcact
    14641 ttacattttg caacacggta taaggattta ataaatgttc aaagatttca aaagccgtaa
    14701 agatttcgta gttttcagtt tgcaacgccg attggtcgtt gtccaaatct tcacctttgg
    14761 tattaatgac cgtgtaacca ttgtcctcca taattttgga aaacggattg ggtacaccca
    14821 aatcaaaaat agtttctgaa ggattgatat gtttttttag aaaatctaac gtaatgttga
    14881 agcgtttgct tggatatgtc ttctcgtaca tagtgactgt gaaaatttac agccgcaaag
    14941 ttacaaagtt ttatcttcta ataatattta gatgaatttt gtttgtgttc agtattttta
    15001 gattgtcaaa ctattgcttc tgatatttgg cttggcaagg ttcaaagcaa ggataattga
    15061 taattaatac ggtgccgttt tgcacataat tatatcctaa agttgtttga acacaagcat
    15121 cggatgtaat agtagcattc gttgcatcgt aagttcccga tgttgggtta agcgttgagg
    15181 tggtcatgtc gcacaaatcg ccgttagaag ttaaggtgcc atcactgtta aaagttatgg
    15241 ttttctcgct gtccacactc ataaaagtgc cactaccatc acctggatct tctaatactt
    15301 ctatcaattt ccaatttcct actatagagg tattcgattt gttgtcgtct ttattacaag
    15361 cactaagcat accaaagacc aaaagggtaa aaaatatctt tttcataatt ggatttattg
    15421 tttagatggt aaaccttctt aatggttgcg ttttaaatgg catagattta gtgtgttgta
    15481 ttttatgttc ttaaaatctt ttccattttc tttcctttgg caagttcgtc tatcagtttg
    15541 tctaagtagc gaatcttttt catcagcggt tcttcaattt cttcaatgcg atagccacaa
    15601 ataactccag tgattttgga agcattagga tggagttgtg cttgcccaaa aaaggtttca
    15661 aaatcattct tgttgtctag ttgttcttgc aaagcttttt catcataacc cgttagccat
    15721 tgaattatag tgtgcacttc ttctttggtg cgtccttttt tctccgcttt attaatataa
    15781 tgaggataca cacttgcaaa cgccatcttg taaatttttg tgttatccat ttagtagttt
    15841 tgtatgtgtt tgatactcca tatttctgtg atttgtatga catcacagaa tctttttatc
    15901 aaaaagattc aattttgatc aaagcaaaaa tagtttatta gttttgcata attctaattc
    15961 atctagagcc agtttatgct ccacttcatt caaaaaactt gaactaacat agctgatgta
    16021 ctaacttata ccttatatac tatggagttc acattcattc cagcgcccac tgatgcaaaa
    16081 agaacaatat cgcctttgcc taacgaatga ttttctacat tgtttttgag taacaaatcg
    16141 tataatgttg gcacggttgc cacactagag ttcccaagtt tatgaatgct cataggcatg
    16201 atgccttcgg gaacagtttg tttgtataag cgataaaaac gttgaacgat agcttcatcc
    16261 attttttcgt tggcttggtg aatcaatatt ttttttactt ggttgatgtc aacaccgctt
    16321 ttttctaaac aacttgccat agcttttggc acttcgctta aagcaaattc atatattttt
    16381 cgaccataca ttttgatata gcgaacatct ggatcgttaa atgtgttaaa agaattccca
    16441 aaatacaaat agttggcttc gtcataggta aacgatgctg attcgtgggc taatattccg
    16501 ccttcttcat tggtagcttc aattatcgta gcacctgcgc catcggagta aatcatagaa
    16561 tctctatcat gcatgtccac tactctcgaa agtgtttcgg caccaataac caaacatttt
    16621 tttgccatgc cagcttttat aaatgcttgc gcttgaataa cagattctac ccaaccagga
    16681 caaccaaaaa gcatgtcata agccacacat ttcgggtttt taatgcgtaa attgtgtttt
    16741 actttagcag ccaaactggg tagcatatca ccttgaatag catttttttt tacatcacca
    16801 aagttatgag caaaaataat atagtctagg gattctgcat caactttagc atcatcgata
    16861 gcaatttttg cggctatagt tgcaatgtct gaggtaacta aatcatcagt tacataacgg
    16921 cgttctttaa tacctgtaat ttctaaaaat ttttgtgcaa taacatcatt cggatgtgga
    16981 aatggtgttc catcatcgtt gaggaacaca tgctttgcaa agtcgatatt agaaacaact
    17041 tctgtgggta tatagcttcc tgagccagtt atttttatat tcatgttggg gaaataatat
    17101 gctaaagtat agaataataa ttaaaatcaa acataaaatt tagtatgcgc gcatattatt
    17161 gtgaaatatg taatatttag gtaagaaaat aaatatttga ttattattta ggcattttgg
    17221 tgcacaattt ttatttgcat aacggtagcc atactcaaag cattcgtttt catttgctct
    17281 gcattttttc ctttaaaatc gttatcgata gttgcataaa gatgtttgag ccaagtttca
    17341 aaatgttctt tggtaaatga atatttggcg ttaatgtctt tatgaatttg aaacatattg
    17401 ttactataac cacctttcat aaaaagagat tgttcccaaa aagtggctag tagttcaaaa
    17461 tgttcctcaa gatgatggtg tacatcagta actttggtaa aataaaaatt gatggtttca
    17521 tcggctaaaa gcttgtcata aaacgttcgc atgatgtaaa gcaaatcctc tcgggtttcg
    17581 atgtcgttca ttgtttaaat ttatacaaag ttaccatttc tgaaatttta ttgtctgata
    17641 ttaatcataa aaaaaggttt caaatttatt tttttgaaac ctttagtctt actacttact
    17701 actaaaatct taatacattt acatatacgc ttcaataggc gcacaactgc aaactaagtt
    17761 tctatcgcca taggtttcat caacacgacg aacgcttggc cagaatttgt tctcagcaac
    17821 ataatctaaa ggataagcag ctttttctct tgtgtatgga aacgtccaag tatcggaagt
    17881 aaccatagct agtgtatgtg gcgagttctt caaaacattg ttaggttctt caatagcagc
    17941 tgcttcaatt tcttgtcgaa tggcaatcat ggcatcacaa aacctattta tttctgctaa
    18001 atcttcggat tcggttggtt caatcattaa tgttcctgct actgggaaag aaacggttgg
    18061 cgcatggaag ccataatcca taagacgttt tgcaatgtca gttacttcaa ttcctttggc
    18121 tttaaagctt ctgcaatcta atatcatttc gtgcgcagct cttcccattt ctcctgaata
    18181 taaaatagga taatgttctt cgagttttgc tttcatgtag ttggcattta atattgcata
    18241 ttttgttgcg ttagtaattc cttcagctcc taacatacag atataaccat aagaaattag
    18301 acaaactaat gccgagccat aaggtgcacc agaaatagca gttattgctt gatcgcctcc
    18361 aacttttacc aatggattgc taggtaaaaa cggcactaat ttttcgttca cacaaattgg
    18421 ccctacacct ggcccaccac caccgtgagg aatggcaaag gttttgtgta aattaagatg
    18481 acaaacatca gcaccaatgg tagctggatt ggttaatcca acttgggcat tcatgttagc
    18541 gccatccata tatactaatc caccattgtc gtgaatgatt tgtgtgattt ctttaatggc
    18601 actttcaaaa acaccatgag tagaaggata ggtaaccatc aagcaagaaa gattttcagc
    18661 attttcgatg gcttttgttc ttaaatcttc tacgtcaata ttaccgtttt ccatagtttt
    18721 ggtcacgata attttcatgc cagccatagc cgccgaagct gggttagttc cgtgagccga
    18781 agcaggaatt aaacatacat ttcggtgact gtttccttca gcaatatgat aggcacgaat
    18841 agccattaat ccagcatatt cgccttgtgc tccagaattt ggttgcaatg ttgttccagc
    18901 aaaacctgta atgacattta gttgttgttc tagttttttc aacattccga tataaccttg
    18961 cacttggtct agtggtgcaa acggatggat agtattccag tatggcatac tcaatggcaa
    19021 catttcggca gcggcattca atttcatcgt acaagaaccc aaagaaatca tcgaatgatt
    19081 taaagataaa tctttgcgct ctaatttttt gatataacgc atcaagctgg tttctgaatc
    19141 atagctattg aaaacgtcgt attgtaaaaa ttctgttttt ctatttaagt gcattggata
    19201 gttgttctcc aacgcatatt cgtttacttc aaaagcttct ttgcctaacg cttctgcaaa
    19261 aactgcaatt acttcattga tgtctttgaa taaaatagtt tcatttaaag cgattgaaat
    19321 ggtttcgtca tcaatataaa agaagttgat ttcgtttttc tcagcaatgg ctctcacttt
    19381 tttggcatca gcttttacaa cgatggtgtc aaaaaaagaa gtgttcgttt gataaacgcc
    19441 aagtttattt aaagcatttg ccaaagtaac tgccgatgca tgaactttgt cggctatata
    19501 acgcaatcct tttgggccat gatatacggc aaacattcct gccattactg ataataatac
    19561 ttgtgctgta caaatgtttg acgttgcttt ttcacgttta atatgttgtt cacgtgtttg
    19621 caaagccata cgaagtgctc ggttgccatt agtatcaata gttactccga taattcttcc
    19681 aggcatcgaa cgtttgtatt cttccttggt agcaaagaaa gcagcatgtg gaccaccata
    19741 gcccattgga attccgaaac gttgtgtagt tccaaccact actgctgcac ccatttctcc
    19801 aggaggtgtt agttttacta aagataaaat atctgcagca acggctactt ttatttcatt
    19861 ttcattggct ttggcaataa atgaggtgta gtcatttact tgaccgtatt tgcctggata
    19921 ttgaagaatg gctccaaaat agtcagaaga aaagtcaaat gtttcatgat ttccaactac
    19981 taattccaca ccaattggtg tagaacgtgt ttgaagaacc gataaagttt gaggtaatat
    20041 ttcttccgat acaaagaatt tgcacacgtt gtttttcttt tggtcacgag aacgcacatc
    20101 aaatagtaat gccatggctt cggcagcggc agtgctttca tctaacaaag aagcgtttgc
    20161 aatttccatt ccggttaatt caataacggt agtttggaaa ttaagaatag cttccaatct
    20221 tccttgagca atttctgctt gataaggcgt gtatgccgtg taccaacctg ggttttcaaa
    20281 aatatttctt tgaatagcag gtggaactat tgttggatga tatcctaagc caatataaga
    20341 tttgaatact ttgttcgttc ttcctaatat tctgatgtga ttggcatatt catattcggt
    20401 cattggagca tcaagttgca attctttgtg caaccgaatg ttgtcaggaa cggtttcgta
    20461 tattaattgt tctaaggttt ctacgccaac ggtttttaac atttctggca aatcgttttc
    20521 tcttggtcca agatgtctaa gtgcaaatgc gtctgttttc attaaagtgt tgtgtcttaa
    20581 aagtgtatag atgtgttcaa atcatcccaa taaattggat aatttttttg cagtacaaaa
    20641 gtaattatta aatccatatt attatgctaa tcaaaatcta atgttttgat attttttcaa
    20701 ctataaagag attttgttaa taaaatagga tttaaagatt ctatcgacaa aatacatcaa
    20761 taaatcattt cgtcgaggat taattacttt ataacgaatt tattaacata atcttacgac
    20821 ttattgaaaa cttatttaat ttcgattaaa attttataca aatgaaaaaa attaccttga
    20881 ctttactatt cttttttgga atggtagcaa cctacggtca aacattggtt cttaatgaag
    20941 taatgacgtc taatctttcg gtaattgccg atgacgacgg tgactataac gattggatag
    21001 agatttatta caatggagca accgctatta atttagaagg atatgcactt tctgatgatg
    21061 ctactctacc aatgaaatgg gtttttccag aatattggat agaaccgggg gatcatttat
    21121 tgatatggtg ttccgataaa aacagaacag acattaataa tgatttgcac acgaatttta
    21181 agattagctc tacgggtgaa gttatttatt ttaatagacc aaatggaact ttacaagata
    21241 gttttctagc agtaatttta ccagaaaaca tctcctatgg aagacaaact gatggtgcaa
    21301 taactaaagt ttattttcaa caatctactc caggtgcagc aaataatact cctggattta
    21361 gtcaagtttt aaatgcgcca tcaataaatg ttcctagtgg gttttttact tcatcttttc
    21421 cagtaacaat aacttacccc gatccaaatg tgacgattgt ttacacatta gatggttcag
    21481 atcctgtttt gacaaacctt gcagggacta attataatta taaaaataag tatatcgaat
    21541 tagcaggaca gactgctgga gcacaacttc aaaatagtta taaatcattt acatattcaa
    21601 gtcctttaac aatagttgat aggtcgagca tagcaaatga tttagcaaaa atgtcatcta
    21661 cttttcaaaa tgtacctagt tatattccaa ctttcaatgt ttttaaagga accgtcttga
    21721 aagttagagc ctttaaaaat ggtgctatac caagtccaat tataacaaaa acttattttg
    21781 taacacctca aggtagtgct agatatacat tacccgttgt ttctttgagt attactgaat
    21841 caaaattctt tgaatataat gatggaattt ttgtagctgg aaaagatttt gatgattgga
    21901 gagctgcaaa cccaacagtt gatgctgatt ttagcagtgt aaatgaaaac gaagaaaatg
    21961 caaattacta tagaattagc gataatgaag aacagaaagg acatttaagt tattttgttg
    22021 gaggacaaga agtattaaat caagatatag gaatagaagt taatggagga aattctagaa
    22081 attttcaaag taaagccttg cgtcttatag cgcgttcgga atatggtaac gataaaatga
    22141 gttacccatt ctttgccaat gaaaattata atagttataa aaggttacta cttagaaatt
    22201 ctggtcaaga ttttagaagc actatgttta gagatggttt gtttcacact ttggttgaaa
    22261 aattaaatac agaaacgaaa tcttaccaac caacaattgt atttttaaat ggagaatatt
    22321 ggggaatttt aaatatgaga gaacgatatg acaagcagtt cttcaatcaa aaatacaata
    22381 tagccgatgg agagttagat tatttaaaaa atgatttaac accagtagaa ggtgatggaa
    22441 atcattatgc agcgatggaa acctatttac agaataattc tttgttaaat acagctaatt
    22501 acaattacat taaaacgcaa ttagatccag aaaatatgag agattattat atttctaata
    22561 tttatgctca aagtacagat tggccaggtt ggaatacaat attttggaga aaaaaaacag
    22621 cagcttatga gccaaatgct ccctttggtc aagatggtag atggagaacc gggataaacg
    22681 atatggatga taccttggga gaatatcctg aaggaagtaa tcataataca ttggcgtttg
    22741 ccacagcaac tggtggacca gattacccaa atccagaatg gtccacttta atcttaagaa
    22801 gactattaga aaacaacgaa tttaaattaa actttatcaa tcgttttgca gatttgttaa
    22861 acacttattt tttgccatca agagtcaatg ctatgattga tatcttcaaa aacaaaatta
    22921 accctgaaat agcagagcat caagcaagat ggaaaccatt ttatgatgta gaaacatgga
    22981 atgattacag tattcaatcc atacgagatt ttgccaacac tagaccaaat aaccaaagag
    23041 cacatattag aggtaaattt ggaattagtg gagatattaa tgctacttta aatgtagatg
    23101 atgtagcaca tggatttata aaaatgaata cggtagaaat aaacagttct acaccaggtg
    23161 ttgctgccaa tccttatcct tggaccggaa tttattttaa aaacattcct gttactatga
    23221 aagcaattgc attaccagga tttgtgttta gtcattggtc aggtgcttct accagtacat
    23281 cagcagaaat tacgcttaca acagcagctg atttttcagt aacagctcat tttgttcctg
    23341 caggaagtgg tggagaagaa gtgccaattt attactgggt atttgatagt tctttagaaa
    23401 ataacactcc tttatccagt gtgaatacaa cttataatgt taatgttctt ccacaaggag
    23461 ttttaaacta tcaatcgtgt ctttctgggt acccttttga tgcttccaat gcaaactgga
    23521 gaaaagcttc catggaaaga agaaacaatc caacggatgt gaactatatc cctgaggtga
    23581 ataacaacct aacttttgca gcttccaata tgagaggaat tcaagtaaaa caacctttcc
    23641 aaaataacgg ccaagaaaat aagttgattt tttcaagttc aacagcaggt tatcatgata
    23701 ttaaagtatc ctttgcagca gttaatgaat tagcggctga tggattagtt gttgattatt
    23761 cgattgctgc tggttctggt acatggataa ctacaggttt agcagcatct agtctgcctt
    23821 taacgagcag ttttcagctg tttactattg atttttcatc tattactgcg gttaataaca
    23881 atccaaattt taaatttaga attcgatttt caggaacaaa tttaacagaa gatttaggaa
    23941 acagagttac ctttaacaac attagtatta aagggaaaca agacaacttg agcgttgttg
    24001 aagcagatag taaattagaa ttcgtcatgt atcctaatcc aactactggt gttttgaatt
    24061 tagtttacag ttatcaagat gtgaattatg caatattttc aatcgatgga aaattaattc
    24121 aaagcggaaa attagataac ccacaaatta atgtgagttc tattcaacat ggaatttatt
    24181 tactgcaact ttcggcagat ggtaaaacag aaactaaaaa gtttattaaa aattaataat
    24241 caaaattttt taaatatttg aagaagaggt ctaattggcc tcttttttca tttaatcgat
    24301 gttattggaa agttgtcgat aatatgcttt attagaaaaa aatcaagtta attttgagtg
    24361 aaatttagaa tttataactt ccttttttat gattaaaaaa atactttttt ttgtaatttt
    24421 tttcactgtt ttttttcaag aaaacacatt ctcacaggca ttagtcatta atgaagtagt
    24481 cacttcaaat agtacaacta ttgcggatga agataattca tatcaagatt ggattgaatt
    24541 atacaataat agtgcaagtg ctataaattt atcaggatat ggaataacag atgattcaac
    24601 caatctgttc aaatgggtgt ttccaaatgt taccataaat ccaggtgggt atatgctcgt
    24661 ttgggcatcg gataaaaatc gaactaatcc tgccaatcca ttacacacaa attttaaaat
    24721 aagttcaagt ggtgaaacaa ttactttaac gaatgcttca agtgtaatag ttgatactgc
    24781 accagcaact gcaatgcaag ccgatgaatc tttgggtaga ttgccaaacg gaacaggcgg
    24841 ttttgtgctt attcaaactc caacgccaaa tgccttgaat gttaatggaa caccaattgc
    24901 aactttagat ccaccagttt tttctcaaaa ttcaggtttt tatacagcag gttttaatct
    24961 aaatataacc actaatgagc ctggggcaac aattatttat acacttgacg gttcagaacc
    25021 tgacgaaaat aatttaagcg gcacaaccta taattacaaa aatcaatacc caaaaatacc
    25081 aggacaagct tttggtactt ttctgttcaa tacctttcaa tcgtttcaat attcggcatc
    25141 tataccaatt aatgacaggt cttctcagcc aaataaaatt tcagccattt ctacaaccta
    25201 tgattttaca ccaccctatt ttccaaatgg accaatttat aaaggaacta ttgttagagc
    25261 aaaagttgta aaaccaggtg cagtttcaag cgaagttata tcaaaaaatt actttattac
    25321 cccagcagga tcaagtaaat atacacttcc agttctatca tttagtttta atgaaaatga
    25381 gttatatgat tatgaagatg gaatagctgt tgcaggtata gattttgaca attggagaac
    25441 tgccaatcca acagaaattc ccgatcgcga aattggaaat tttgaaagac gtggtgctgc
    25501 caatgaacga aaagccaaca tgcattattt tgtaaatggt cttgaaaaaa ttaatcaaga
    25561 tgtagggata cgaattagag gtgcttattc aagattatac ccaagtaaat catggaatat
    25621 ttatgctcat tccgaatatg gtgatggcaa tttgtcttat aatttttttg ccgacgaacc
    25681 ctatacaagt tatgagagat taagcgcgaa aagtggcgga agtgattttt acaacactat
    25741 gtttagagac gctttaaatc atcaattggt gaaggattta aaaatggaaa cagaatcttt
    25801 tcaaccaatg gtagttttta tgaatggaga atattgggga ttactcaata tgagagagaa
    25861 atatgatgat aattatttca aacaagttta taatattgac aatgttgatt tattagaaaa
    25921 tcaaggagca attcaagaag gtgatgctgt tgattacaat aatatgtatg catatattca
    25981 aaacaatagt tttacaaatg aagcaaatta tacatacatt caaacgagaa tggataccga
    26041 taatttcata gattatttta tcacaaatat ctttactcaa aatacagact ggccaggaaa
    26101 taatattatt tattggagaa aaagaacggc acaatacgaa ccaaatgcac cttatggtca
    26161 tgatggaaga tggcgatggg cacttcacga taatgatagt acattagatg atgcaagtta
    26221 taattcgtta cataatgcaa catcaactgt tgatgttggc ccaaatccag cctggtcaac
    26281 cttaatttta agaaaattat tagccaatac aacttttaaa aatgacttta ttaatcgttt
    26341 tgccgatttg atgaatacta acttcctctc ttctagaatt attgcaaaaa ttgatgagat
    26401 ttcagcgata attgcacctg aaatgaatga tcaatatttt agatggaaag caccagttga
    26461 caatggtgat tggcagtacc atcttgagga ggaaagagaa tttgctgaag acagacctgc
    26521 atatcaaaga aatcatattc gttctaaatt tggaattaca aataatatta atgcaaattt
    26581 gaatgtttca aattatgacc acggctatat taaaataagc acaattaata ttcaagatgg
    26641 aactacagga attattggta acccatatcc ttggacggga atttattttg cggatattcc
    26701 aattaaatta aaagccaatg ctagaccagg atatttattt agtcattggg aaggattttc
    26761 gacaagcacc aacgaagaaa ttacaataac gacaaaccaa agtttttcga ttactgccgt
    26821 ttttattcca gaaggttttg cagtggaaac gcaagagcca atttattttt ggttgtttga
    26881 tggagctatt ccaaacgata ctccattaac gagtttaaat tcaacttttg aaattccagt
    26941 agaaggtgtt ttagaatatc aatcatgctt gacaggttat ccatttgtaa acggtaatcc
    27001 aaattggaga aaagcatcca tggaacgaaa aaatagtcca acaaacatta attacattcc
    27061 agaggctaac aataatattc cttttgcttc ggcaggaatg cgaggaattc aaataagaca
    27121 accatttcaa gacaatggtt tagagaatac aatacaattt aatctctcaa caaaagacta
    27181 taaggatatt aaatttggat ttgcagctaa aaatgataat gcagctgatg ctattattat
    27241 tgattattca ataactgcag gaactccaac atggattaca acaggtttag catcaagttc
    27301 gttaccattg tcatctactt tcgaattgtt tgaagttgat ttttcttcaa taattgccgt
    27361 aaataataat gcaaatttta aaatacgttt aaggtttacc ggctcaaaca tgactgttga
    27421 caacggagat gaagttgttt ttaataattt ttcattaaaa ggagttcaaa ttcctttgac
    27481 ctatgttaca ccaaacgttt atacaataga cactccaatt tcggatttaa ttcctaatct
    27541 aactgcttca gcagatagtt ttgctatcag tccagcagta ccaaatggac taacattta
//
LOCUS       DAOOBH010000019        27597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_2278_length_27597_cov_6.464273, whole genome shotgun sequence.
ACCESSION   DAOOBH010000019 DAOOBH010000000
VERSION     DAOOBH010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 27597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27597
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_27597_cov_6.464273"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PK218_03775"
     CDS             <1..304
                     /locus_tag="PK218_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HPW97660.1"
                     /translation="NNFKHFYGYDYLSVSANSFIKSNFTLDYEIFKKNHLNFSANYAN
                     LEDGLFASGNWFSKPKLSGYAFGYGIESIVGPIEVKYSWSPELPKGYVWVSAGFWF"
     gene            complement(301..459)
                     /locus_tag="PK218_03780"
     CDS             complement(301..459)
                     /locus_tag="PK218_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97661.1"
                     /translation="MNTEALITMITTQGIVIGFTGYFFYRVLTTKPKPEPDSYTENDD
                     VVERQPEN"
     gene            complement(479..2149)
                     /locus_tag="PK218_03785"
     CDS             complement(479..2149)
                     /locus_tag="PK218_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPW97662.1"
                     /translation="MAASSQAWGSRVGLILAMAGNAVGLGNFLRFPVQAVQNGGGAFI
                     IPYLICFLLMGIPLLYIEWSSGRFGGKFGNHSTPYILDSMVKGRILKYIGVFGIFTNV
                     AVAAYYCYIESWTMSYVYHSLRGTFNGMSQHGVADFFNNYVDISKSSTGIPYEAVVFY
                     ILCLLLNTFILSKGLKGIEKVAKIGMPLLILFGAILAIRGLTLGTSGASELFPNANAW
                     DGLNFLWTPQYDSILDLKVWMAAAGQIFFTLSVGMGTIHCYAAYMNSKDDIALNAVSA
                     GFMNEFVEVVLGSLIVIPIAAGYLGLDWVLENAGFGMAFQTMPYLFQQWGPMLAAIAG
                     LLWFGLLFFAGITSSLAMGTPWIGFMRDEFNWGQNKGAWSFGLLILVMGLPTVIFFQE
                     GVFDEYDYWAGTVSLVVFAMLETILFSWIFGMKKGWAEITSGADIQVPLIYKFIIKFV
                     TPVMLIIIFLGSLFKPLNNDWSGNVSSLLSGNGWSLDNGSIIKTITHAGIKEKIMAAT
                     DPEIIGQLESQILYLNFARILLLGLFVFISALVYVAFLKRRREGRATL"
     gene            2583..3743
                     /locus_tag="PK218_03790"
     CDS             2583..3743
                     /locus_tag="PK218_03790"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HPW97663.1"
                     /translation="MPFYHKLGNIPPKRHIQFRKPNGDLYYEQLFGTVGFDGMYTNSY
                     HEQRPTKVKEIRAQYSVKPSISKSNNIQSYKLKGFEVTPQDDYLESRKIVLTNSDCHI
                     VLAAPRKSTNDYFYKNSDSDEMIFVHKGSGKLRTMYGNLDFKYGDYLIIPRGIIYKID
                     FDTIENRLFIVESNVPIYTPKQYRNWFGQLLEHSPFCERDIRRPQELETNNESGEFVI
                     KVKKKDEIFEMVYASHPFDVVGYDGFNYPYAFSIHDFEPITGRIHQPPPVHQTFETSA
                     FVVCSFVPRMYDYHPLAIPAPYNHSNIDSDEVLYYVDGDFMSRNDIEQGHISLHPAGI
                     PHGPHPGAAERSIGKTETQELAVMVDTFKPLMVTDEAMKIADDTYYQSWLDH"
     gene            3861..5021
                     /gene="hppD"
                     /locus_tag="PK218_03795"
     CDS             3861..5021
                     /gene="hppD"
                     /locus_tag="PK218_03795"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HPW97664.1"
                     /translation="MSKEINNVEYGLEKIFEGAQDFLPLLGTDYVEFYVGNAKQAAHF
                     YKTAFGFQSHAYAGLETGMKDRASYVLKQDKIRLVLTTALKSDSPIGEHVKKHGDGVK
                     IVALWVEDARSAYEETMKRGAKSYMEPTVETDEHGEVVRAGIFTYGETVHLFVERKNY
                     TGSFLPGYKEWKSDYNPAPVGLKYIDHMVGNVGWNEMNTWVKWYEDVMGFVNFLSFDD
                     KQITTEYSALMSKVMSNGNGRIKFPINEPAEGKKKSQIEEYLDFYEGSGVQHIAVATD
                     DILTTVADMRSRGIEFLSTPPQAYYDAVPLRLEAHHHELKEDINKLMKLGIMIDADEE
                     GYLLQIFTKPIEDRPTLFFEVIQRMGARGFGAGNFKALFESIEREQALRGTL"
     gene            5517..7214
                     /gene="recJ"
                     /locus_tag="PK218_03800"
     CDS             5517..7214
                     /gene="recJ"
                     /locus_tag="PK218_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPW97665.1"
                     /translation="MRWNLKPKPSSEKVTQLAKELKVEELVATLLIQRGIETFEEAKA
                     FFRPSLDDLYDPYLMKDMDKAVERIEKGIENLENILVFGDYDVDGTTAVSLVSSYLRS
                     LYPNVATYIPDRYDEGYGISFKGIDFAEDNGFSLIIALDCGIKSIDHVEYAREKNIDF
                     IICDHHRPGNELPKAVAVLDPKREDCNYPYDELCGCGVGFKLVQALGQNRNQTINDLM
                     VYLDLVATAIAADIVPMTGENRILAKFGLEVINTKPRPGIKALIQNVKKKELTITDVV
                     FIVAPRINAAGRIKHGNEAVALLTEYNIEQAEQFAAEIEQHNNERKELDKHITTEALC
                     QIEENNEQEKFTSVVFQENWHKGVIGIVASRLTETYYRPTVVFTKSGDKLAASARSVK
                     DFDIYNALEACAEHLEQFGGHAYAAGMTLKKENYKAFKAAFEAEVQKTIPPELLIPEI
                     SIDAQINFSEINPKLIRILNQFEPFGPQNMTPVFMTKNVVDTGYGKTLGLEDEHLKLF
                     VKQDNSEGIGVIGFGFGNQLELTKNRKPIEICYCIDENEFNGNVSLQLRLKDIKQYN"
     gene            7247..8515
                     /locus_tag="PK218_03805"
     CDS             7247..8515
                     /locus_tag="PK218_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW97666.1"
                     /translation="MIKNDPYEALRFTEFKFFLAMRFAMVFAWSMQFVIIEWEVYKLT
                     KDPLSLGIIGLMEIIPAIGMALFAGHIVDQNEKKNLLVKCILGFSVISFGLFLITVPS
                     ITKDLSLQTTLYVIYGLVFFGGLVRSFIGPTIFSLLSLIVPKKTYPNAATWSSSTWQM
                     GSMFGPAIAGFSISLFGVHWSMCLVFACSLFALLFLSKIEKKPILNPKIGEPIFQSLA
                     EGVKFVFKNKTILGAISLDMIAVLFGGAMALLPIFAQDILKVGSEGFGILRAAPAVGS
                     VITMVLSAYITLNRKAGMKLLTAIFMFGVCIIIFGLSTIFWVSVLAMFFSGIFDGVSV
                     VIRNTILQLHTPDNMRGRVASVNSIFVGSSNELGAFESGVTAKLMGTVTAVVFGGTMT
                     LITVVFTGIALPSFRKLDLQKDVEALEKQE"
     gene            complement(8516..9274)
                     /locus_tag="PK218_03810"
     CDS             complement(8516..9274)
                     /locus_tag="PK218_03810"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPW97667.1"
                     /translation="MQLSHNKKIYFASDQHFGAPTPEASFPREQKFVSWLDEVKKDAE
                     CIFLLGDLFDFWFEYKTVVPKGFVRVLGKLAEIRDSGIPIYFFVGNHDLWMHDYFEKE
                     LNIPVYHDNQEYVFNGKSFLIGHGDGKGPGDKGYKRMKKVFTNPFSKWLFRWLHPDIG
                     VKLAQHLSVKNKLISGDADVKFLGEENEWLVQYSKRKLETKHYNYLIFGHRHLPMVLK
                     VGENSEYINLGDWIGYFTYGVFEGEKFELKVFEN"
     gene            complement(9347..9799)
                     /locus_tag="PK218_03815"
     CDS             complement(9347..9799)
                     /locus_tag="PK218_03815"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPW97668.1"
                     /translation="MNKIRITKQFSFETGHALYGYDGKCKNVHGHSYKLSVTVIGTPI
                     TDSNNVKYGMVIDFSDLKKIVREEIVDLFDHATVFNQNTPHVELANELKNRGHHVILV
                     NYQPTSENMVIDFAEKIKSRLPKEILLHSLKLQETESSFAEWYASDNV"
     gene            9906..9979
                     /locus_tag="PK218_03820"
     tRNA            9906..9979
                     /locus_tag="PK218_03820"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9940..9942,aa:Ala,seq:ggc)
     gene            complement(10116..10949)
                     /locus_tag="PK218_03825"
     CDS             complement(10116..10949)
                     /locus_tag="PK218_03825"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HPW97669.1"
                     /translation="MKKIKTLFYLLTFFAIFSCSDTKVIENLPADTEDAEPVPGYFQK
                     NVLIEDYTGTWCGNCTRVAYAIDQVHLQTDKVVTVAIHNGNDPYHFENFMPLKDLIMP
                     DSPALELPQSRLNRTITWLSPEPSNIQQAKGLTGNNSGLGLAISSSISNGMVNVEVNM
                     KFVQNYSGLKLVVYLLEDHLIRNQVNYTSYYGNVNPVPNYEHNHVLRLNLTDLLGDPI
                     TENTTAGQQITKSFSIPVPSNISNPDNISFAAFIVQADNLSLNARHADLNENQTYQQN
                     Q"
     gene            11044..11523
                     /locus_tag="PK218_03830"
     CDS             11044..11523
                     /locus_tag="PK218_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPW97670.1"
                     /translation="MKNLVTLFLIFAGIIAYSQTKMPDVSLSDIDGKKVSIKNDFNEK
                     DKIYVFSFWATWCTPCINELDEMNDVKGEWTKNLNIEIIAVATDDSRTQKRIKPLVNG
                     KGWDYKILLDSNQDLKRALSIVNIPYTVVVKNGEIVHIQNGYVPGSEDELYTKLKTL"
     gene            11527..13200
                     /locus_tag="PK218_03835"
     CDS             11527..13200
                     /locus_tag="PK218_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HPW97671.1"
                     /translation="MKKIIFLLFFGTTFFVNGQDKGTVFGGFETNAQWYLNDKTLYDE
                     FYNSVSQPENPVRSNSYLFVNYKIKNWSFGVQGEAYEQDALLNYNPKYKGVNLATYYA
                     RFKMEKIDVTAGYFYEQFGSGLLLRTWEDRALGINNALRGGRIIFKPVSSFTLKAVYG
                     QQRTGFDVSNGKIYGVDADISLSDLFKFENSDLGIGFSYVGRDEKTDIVNPKFNDVTN
                     GFAGRINYSHGSFYLSTEYDYKSEDAVVQVRNQIDNNLIKPGSALLFNMGYSKRGLGI
                     DGTFRRLENMSFFSERIAKGNSFNDLIMNYIPSLTKQHHYNLANIYVYQAQPNVILND
                     VSIVKAGEIGGQIDLFYNFKKGSKLGGKYGTKVAVNYSQYNALGGKFYIYTPQDYQTD
                     FFGFGKKYFSDVNVEITKKWNNKLQTVLSYINQYYNKKLVEETYGEVKTNIIGAEATY
                     RFNDTKSLRLQGEHMWADSDKKNWAALTAEVNLGTKYTIFASDMYNYGNDDPKLRINY
                     YTIGNSYRYKSTRVSLSYGRQRGGLVCVGGVCRYVPESTGVSLSLNTSF"
     gene            complement(13413..14165)
                     /locus_tag="PK218_03840"
     CDS             complement(13413..14165)
                     /locus_tag="PK218_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97672.1"
                     /translation="MKKIIVSLVCLISAMSFAQMTMKKLDGTPLNDGDVIAYSVLGDP
                     NDISSSDPAYLGFKIYNSSNSSISVKMKCISITGGATGTNLQFCIDPICVGTISVGNS
                     YPSNGVSTVPANGQNGNFDHFVNFDAGTGNSIVEYVLKFYMVNQFGGETGSSITLTYR
                     YNPNLNTSGFSSLNNAGINISSTLVKSQIEFDATSIGTAQLYDLNGKLVNNINFVSGY
                     NTINVSNLNKAVYVLNFTTQDGKNASVKVIKQ"
     gene            complement(14175..16040)
                     /locus_tag="PK218_03845"
     CDS             complement(14175..16040)
                     /locus_tag="PK218_03845"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HPW97673.1"
                     /translation="MKKITLLFLLSAFCGFSQTYYSNDFESGLAGWTANDLNNDGQGW
                     SILNASSLNSALGSGSLMSFSYVNQVGITPNNLITSPSIDLTSVTGSNLFLFYDQLTN
                     TNYPDEHYAVYVTTSNDPATIVASTPVFETTVSNGSLVTKSINLTSFIGQTVYVSFRH
                     FNCFDQYYLVIDNVKIKTLLENDVQLVSAKLNRFGLLNSSNTITYTVKNTGSNPVTNL
                     TINWNDGTNDHILTVNKNIAVGATGTVNHPTPVQYSTVVEKDLALSITQVNGSADADP
                     SNNTGTKNFNTVSQASPKKVLFEEGTGTWCGWCPRGAIAMNYMDTTYPNEFIGIAVHN
                     EDPMMLPEYDGGAGFSGFPGMNVDRIVLGADVSQSTMVTNLNSRKTLTTPVSLVATGS
                     VNGSNITINANATFRTNFSNANYKLGVIITESNVTGTANGYKQTNYYAGGANGVMGGY
                     ETLPNPVPANQMVYNHVGRALLGGYSGQDGSVPTTITDGQTITYTFNYTVPATSNINN
                     MHAIVILIDQNNGEIINAKSAELATLAVSQNNIAPSFDIYPNPTSDFFNISNLKSGDY
                     TINIYDMSGRLIQTNKKEVIENQELTIAVKGISRGEYIVNIASENSSFSKQLLIK"
     gene            complement(16233..16805)
                     /locus_tag="PK218_03850"
     CDS             complement(16233..16805)
                     /locus_tag="PK218_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HPW97674.1"
                     /translation="MFQLGKTIVSEDILEKDFVCNLSACHGACCVDGDAGAPLTEEET
                     TILEKIYPKVKPFLRKEGIEAIELLGKWVKGTEQDLETPLIDNKDCAYVIFDGKTALC
                     GIEQAYNQGIIEWKKPVSCHLYPIRVKDFSEFAAVNYDRWDICNPACSLGKELEVPIY
                     KFVREALIRRFGENWYAELEKVAKDLKNEK"
     gene            16901..17476
                     /locus_tag="PK218_03855"
     CDS             16901..17476
                     /locus_tag="PK218_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPW97675.1"
                     /translation="MNFDFREIITATMVLFAVIDIVGSIPIIIDLRSKFGHIQSEKAS
                     LVSAGIMIAFLFVGEEILKLIGIDVNSFAVAGSFVLFFLALEMILGIRLYRDEQASSA
                     SIVPIAFPLIAGAGTMTTLLSLRAEFQPINIIVAILLNIILVYVILKSSSKIERMLSK
                     NGLSIIRKVFGVVLLAIAVKLFATNVKGLFI"
     gene            17515..17712
                     /locus_tag="PK218_03860"
     CDS             17515..17712
                     /locus_tag="PK218_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97676.1"
                     /translation="MKLFTNILIVLALGLIIFNITLLDFQNPFKGDSAVALIGIAAAF
                     CAVLILLIFRMSKKIEEKMKN"
     gene            17738..18346
                     /locus_tag="PK218_03865"
     CDS             17738..18346
                     /locus_tag="PK218_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW97677.1"
                     /translation="MFDVLIIGGGVSSMSCAYILGSGFQKPFASDKKIGIIAHQKTSS
                     LQNAIFNNAYGIPSGKLGAELLIESIQHLQENYPHVLQIKDEKVLKIEGIPGDFIVTT
                     NKQNYAAKIIVLGIGASNPFTIEGLAQYIIPHKKANPEKNKIQLQNDDHLVTTGIYAC
                     GTLAGHRSQLTIAAGSGAAVATDILSLWNDGIHVQVHDSVGK"
     gene            complement(18619..>19758)
                     /locus_tag="PK218_03870"
     CDS             complement(18619..>19758)
                     /locus_tag="PK218_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97678.1"
                     /translation="YAGHFLIEAEKKGYVMPINFKNKWISYQQRMAKQWRFDKQYGND
                     LAQSYRLYTLAVAGSPDLASMNRLRETVGISNESKLRLAATYALIKQNSAGLALLSKS
                     FIDDQTDEYYYSYYGSRERNRAMALETLILLGQKQKAFEMATKLAKSLSSNQWMSTQT
                     TAYGLFAMSKFALNNGTKGIIAQYTNGGKTVGINTQKTISQRDLVITSSKNSVTIKNK
                     KNNTLFVRVLNSGILPVGKEQVMQNNVKASVAFVDRKGNSVSVSKIKQGTEFIAEVTI
                     YNQKNERVDNIALTQILPSGFEIVNTRYTEFGEATTNNADYIDIRDDRANYYFGLKAY
                     ETRKFKILLNASYLGNYYLPGLQCEAMYDNTFLARTKGQWIEVVK"
     assembly_gap    19759..19858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19859..>21138)
                     /locus_tag="PK218_03875"
     CDS             complement(<19859..>21138)
                     /locus_tag="PK218_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPW97679.1"
                     /translation="DVASTNYSPYVTYVGIKSPEPNRYGMLETDKNNRFDIATLDENG
                     RPKAVSNLDVRVYRLQSRWWWDASNDNLSNYSTSNSTLAYKNFKINTNSAGKGSVAFS
                     IPENDWGSYLVRVIDANGGHATGTKIYVDSPYWSAKSRNSDGESATMLRFSTDKTNYN
                     VGEKAKIFFPSSEGGRALISIENGTKVLKTLWAKTKKGETTVELPVTGDMAPNVYINI
                     SLLQPHASTKNDSPIRMYGIVPIEVVDKNTVLEPQISMPEVLRPEQTIAVKVSEKNKK
                     AMTYTIAVVDDGLLDLTRFKTPNAWDNFYTREALGVRTWDIFDDVIGAYGGKVNQVFS
                     IGGDADLGGGKAKKANRFQPVVLYFGPFELGKGETKTTKIKIPKYIGSVRTMVVAADA
                     KTSAYGKAEKTTAVRSPLMVLASLPRKISPSEKVT"
     assembly_gap    21139..21238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21239..23616)
                     /locus_tag="PK218_03880"
     CDS             complement(<21239..23616)
                     /locus_tag="PK218_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPW97680.1"
                     /translation="MKNCKVFCLLIALVLSQSCSKKSAADFDSDFALFKEYITGFSSG
                     YVSVHSDFRVQLAFNKSDWKPNQELDDDLFEISPSVSGKVVALSSNTIAFIPKEKLEQ
                     NTLYQITLKLSKLTNKVPKELSNFNFSVKTIKQDFIVNTLDLQSYNKDTYYLNGVIKT
                     ADKLSFEDAQKLVNVTQNNEKLKVTFDKGMNSGLEFKFTIENIKRFDDDTTINIAWDG
                     DDVDIDQKGSVDFQIPGKNQFKVLKIDIGDTNNQSILINFSDPLKKDQDFKGLVAIEN
                     TDNLKFTTMGNVLKVFFNNEGEKSKVDNTSVSNTISDNVAVDSTSVVEPYYEEEETAE
                     KSFYGSRLVEVFQGIENSDGYKMKANYSEKILFEQIKPGVKFLNSGTILPSSNNLKIN
                     FEAANLNAIDVKVYKIYKNNILQFLQDNELNGSRNLKQVSQAVAKKTIPLKQNNLLDY
                     SKWNTYALDISKIIKPEPGAIYRVEFSFKKSYSMYKCSNDSSESGNERESDYEEEDEN
                     DVNYSYNNYDYYYEDDYEWRESLDPCTDYYYFRNTNVALNILASDIGVIAKRGTDKSI
                     AIAVSNIVTTEPISEATVDLYNYQQQKIASSKTDGDGFASFQLEKYAYFAVVTKGDNS
                     TYLKLDDELSLSVSNFDVAGETLQKGLKGYIYGERGVWRPGDTLHLAFILNDNANKIP
                     SKHPIKLKLNDPRGKTTFQKVGTMNTLNHYKFTVPTDADAPTGSWEAVVSVGGARFYK
                     NIKIETIKPNRLKIKNSFNEPILSASKENFNTIQVLWLHGAVAKDLKTEVQA"
     gene            complement(23746..24189)
                     /locus_tag="PK218_03885"
     CDS             complement(23746..24189)
                     /locus_tag="PK218_03885"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPW97681.1"
                     /translation="MENPFTDEYFMKKALQEAEIAFDKGEIPVGAVIVIDNKIIARTH
                     NLTELLNDVTAHAEMQAITSAANFIGGKYLKGCTLYVTLEPCQMCAGALYWSQISKIV
                     FGARDEQRGFLTLGTKLHPKTEVKQGVLAEQASELMIKFFASRRK"
     gene            24227..26002
                     /locus_tag="PK218_03890"
     CDS             24227..26002
                     /locus_tag="PK218_03890"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPW97682.1"
                     /translation="MSKKLLDSINKPSDLRKLETAQLPQLAKELRDFIIDIVSVKEGH
                     LGASLGVVELTIALHYVFNTPNDLLVWDVGHQAYGHKILTERKNKFHTNRQLGGISGF
                     PKRGESVYDTFGVGHSSTSISAALGMAIASQLKGEKRNHIAVIGDASIASGMAFEGLN
                     HAGVTDANLLVILNDNAIGIDPSVGALKNYLTSVKEGKNPKQNNMIKSLNFDYSGPID
                     GHDITALISELERLKKVKGPKFLHIITTKGKGLQQAEKDQVKYHAPGKFDAATGEILP
                     KSEENLPMKFQDVFGLTLVELAKKNKNIIGITPAMPSGSSMKFMMDAFPDRAFDVGIA
                     EQHAVTLSAGMATQGMIVFCNIYSTFLQRAYDQIIHDVALQNLPVIFCLDRAGIVGED
                     GATHHGVFDLAYLRCIPNMIIYAPKDELDLQNILYTASLGLNHPIAIRYPRGRGKNPN
                     FNISNSKFQTIEIGKAHLLKKGTKVAVLSTGFIGNNVSKALESIQNKNDFAHYDFSFV
                     KPLDEMLLHEIFGKHTSIITIEDGVIKGGFGSAVCEFAVANNYTNSVKVLGIPDAFIE
                     HGTIDELQQICKIDVKSLEILFSKY"
     gene            26041..26982
                     /locus_tag="PK218_03895"
     CDS             26041..26982
                     /locus_tag="PK218_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPW97683.1"
                     /translation="MKFFNLRLVLFLLFIATTSFSQNQNVTKKDSLWERKNTVGLDIS
                     QIAFVNWNAGGVSSISGLLKGDFSRKYKRKNLKWVNELIVRYGVNKQDGIELRKTDDA
                     IKFNSTIGHRKDSLSLWYSTAKFEFSTQFTNGYSYPNTTNAISKPFAPAYIFLGVGSE
                     YNDKKNNLTVYVSPLTMKSTLVLDQRLANQGAFGVTKATYDVDGNLLTEGELYRFELG
                     FLVTSQFKKELYKNVIFENRLSLYSDYINKFGNVDVDWQFKFDLVVNEYIKANIGTHI
                     IYDDDIKAKEEVNGEQVTIGPKIQLKQMLGVGLVYNF"
     gene            complement(26979..>27597)
                     /locus_tag="PK218_03900"
     CDS             complement(26979..>27597)
                     /locus_tag="PK218_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HPW97684.1"
                     /translation="ERHPLAFLVEAADDICYTIIDFEDGINLGLVSEDYALEYLIKLV
                     KDTIDTKKYQTLTTKEDRISYLRALAIGNLINDAVRVFLENEEAILQGKFHFALTDKS
                     KYKAQMDDIIKLSVKNIYQSREVIEKELSGYQIINNLLDKFISAFNNNHEGKTTNYDK
                     LLLKILPEKHHLEKDNLYERLLHICHYISLLTDGKALELNRIITA"
BASE COUNT         8879 a         4572 c         4735 g         9211 t
ORIGIN      
        1 aaataatttc aaacattttt atggctatga ctacctaagt gtatcggcaa atagctttat
       61 caaatccaat tttacgttgg attatgaaat ttttaaaaag aatcatctta atttttctgc
      121 aaattatgcc aacttagaag acggattgtt tgcttcaggc aattggttct caaaaccaaa
      181 gctttcaggt tatgcctttg gttatggaat tgaatctatt gttgggccaa ttgaagtaaa
      241 gtattcttgg tcacccgaat tacccaaagg atatgtttgg gtaagtgctg gattctggtt
      301 ttagttttca ggttgacgtt ctactacgtc atcgttttca gtataagaat caggttcggg
      361 ttttggtttt gtggttaata ctctgtaaaa aaaataaccc gtaaaaccga ttacaattcc
      421 ttgagttgta atcattgtta ttaatgcttc tgtgttcata atcttctttt tgtttaaatt
      481 acaaagtggc tcttccttct cttcttcttt tcaaaaacgc aacatatacc aaagcactga
      541 tgaaaacaaa cagccctaat agtagtatcc tagcaaagtt caagtaaagt atctgacttt
      601 ccaattgacc gataatttct ggatcggttg cagccataat tttctcttta ataccagcat
      661 gggttatcgt tttaataata gaaccattat ccaaactcca tccattgcct gataacaaac
      721 tggaaacatt tcctgaccaa tcgttgttta gtggtttaaa caatgaaccc aagaagataa
      781 tgataagcat cacaggtgtc acaaatttaa tgataaactt gtaaatcaat ggcacttgta
      841 tgtcggcacc actggttatc tctgcccagc ctttcttcat tccaaatatc caagagaata
      901 ggatggtttc taacattgca aataccacaa gacttaccgt tcctgcccaa taatcgtatt
      961 cgtcaaagac accttcttgg aaaaagatta cagttggcaa tcccatgacc aaaattaata
     1021 atccaaacga ccaagctcct ttgttttgtc cccaattaaa ttcatctcgc atgaagccta
     1081 tccatggtgt tcccatagcc aaagacgatg tgataccagc aaagaacaag agtccaaacc
     1141 ataataaccc tgcaatggca gctaacattg gtccccattg ttggaataaa taaggcatgg
     1201 tttggaacgc cattccgaag ccggcatttt ccagcaccca atccaatcct aaatagcccg
     1261 ccgcaattgg aataacgatt aaactaccta gcactacctc aacaaactca ttcataaaac
     1321 cagcagaaac agcgtttagg gctatatcat ctttggagtt catataagcg gcatagcaat
     1381 gtattgttcc cataccaacc gataacgtga aaaatatttg tccagctgct gccatccaaa
     1441 cttttaaatc taaaatagaa tcatattgtg gcgtccacaa aaagtttaga ccatcccaag
     1501 catttgcatt tgggaaaagt tctgacgctc cacttgtacc taaagtcaaa cctctaatag
     1561 ctaaaatagc tccaaacaaa atcaacaatg gcattccaat tttggcaacc ttttcgatac
     1621 cttttaatcc tttggaaagg ataaacgtat tcaatagcaa acacaaaata taaaacacta
     1681 ccgcttcgta aggaattcca gtactagact ttgatatatc tacatagttg ttaaaaaaat
     1741 cggctacacc atgttgactc atgccattaa atgttccccg tagcgaatga tagacataag
     1801 acattgtcca agattcaata taacaataat aagcagcaac ggctacatta gtaaaaattc
     1861 caaagactcc tatatatttc aagatgcgtc cttttaccat cgagtctagt atataaggcg
     1921 tgctatggtt tccaaatttt cctccaaatc gacctgaaga ccactcaata tacagcaaag
     1981 gaattcccat taacaagaaa caaatcaaat agggaatgat aaacgcacca ccaccgtttt
     2041 gcacggcttg caccggaaat cgaagaaagt tacctaatcc tacggcgttt cccgccattg
     2101 ccaaaattaa tcccactctt gatccccacg cttgtgaact tgctgccata atttgatttt
     2161 ggtatagtta atgaaatcca aagataaaaa aacaatcgta aaaagatata aaatcaataa
     2221 aaagcttaaa ttgtataaac aaaccctagc atattaacag tttttatacc cattaggcaa
     2281 accctcaaaa tcgcattaac gctattattc tttacaataa ttacactttt aataccttat
     2341 tgtcaaagtt tatgacttct tttgaagcag gaaattcaaa tttttacgat atttgtaact
     2401 atgaaacatt gggacaatct tcttatccat ttaaaattcc tcatcaagag aaatccagag
     2461 tgaatttcaa ttagataaag cgtcagttag acaatgagta aatgaacttc aatctcatta
     2521 tctaacggat taattatcgc attacctaat taactaattg tcttattaac aaataacaaa
     2581 ccatgccatt ttatcataaa ttagggaaca ttccacccaa acgacatata cagtttcgca
     2641 aacctaacgg cgatttgtac tatgaacaac tgtttggaac cgtgggtttt gacgggatgt
     2701 acaccaactc ctatcatgag caacgcccta caaaagtgaa agagattcgc gctcaatata
     2761 gcgttaagcc ttccatttct aaatcaaata atattcaatc gtataagctg aaaggctttg
     2821 aggtaactcc tcaagacgat tatttagaaa gcagaaaaat cgttttaacc aattccgatt
     2881 gtcatatcgt tttagctgct cctagaaaat caacgaatga ctacttttat aaaaattctg
     2941 actctgacga aatgattttc gtgcacaaag gctctgggaa actcagaacg atgtatggta
     3001 atctagactt taagtatggc gattacctaa tcattcctcg agggattatc tataagatag
     3061 actttgatac catcgaaaac agattgttta tagtagagtc taacgttcca atctatactc
     3121 caaaacagta tcgaaattgg ttcgggcagc tcctggaaca ttctcctttt tgtgaaagag
     3181 acattcgccg tccgcaagag ttggaaacta acaatgaatc gggcgagttt gttatcaaag
     3241 taaaaaagaa agacgagata tttgaaatgg tatatgcctc gcatcctttt gatgttgtgg
     3301 gatatgacgg tttcaattat ccttatgctt tctccattca cgactttgag cctataactg
     3361 gaagaattca tcaaccacca ccagtacatc aaacctttga aacgtctgcg tttgtggtat
     3421 gctctttcgt gccacgtatg tatgattatc atccgttggc tattcctgcg ccttataatc
     3481 atagtaacat cgattcggac gaggtgttgt actatgttga tggcgatttt atgagtagaa
     3541 acgatattga acaaggtcac atttcacttc acccggctgg aattcctcat ggaccacacc
     3601 caggtgcagc cgaaagaagt attggcaaaa ccgaaaccca agaacttgcc gttatggtag
     3661 atacttttaa acccctgatg gtaaccgatg aggctatgaa gattgctgac gatacctact
     3721 atcagtcgtg gttggatcat tagaaagaaa agaagaaaga aaatagaaaa agaaattcat
     3781 ctattaaaat gaaatacaac actttgaaag tattcaataa aaattttaac acttaatact
     3841 ttcatcataa tacaactaat atgtcaaaag aaattaataa tgtagaatac ggtttagaga
     3901 aaatatttga aggtgcgcaa gactttttgc cactcttagg aaccgattat gtagagtttt
     3961 atgtcggtaa tgccaagcaa gccgcgcatt tttacaaaac agcgtttgga tttcaatcgc
     4021 atgcctatgc tgggttggaa acaggtatga aagaccgtgc ttcctatgtg ttgaaacaag
     4081 ataagatacg tctagtactt actactgcgc taaaaagcga ctctccaatt ggcgaacacg
     4141 ttaagaaaca cggcgatggt gtaaaaatcg tagcactatg ggtagaagac gcgcgttcgg
     4201 catacgaaga gactatgaaa cgcggagcca agtcgtatat ggaaccaact gtagaaacag
     4261 atgagcatgg ggaagtggta cgagctggaa ttttcaccta tggagagact gtgcatctat
     4321 ttgtggaaag aaagaactat acaggaagct tcttaccagg gtataaggaa tggaaatccg
     4381 attacaatcc agctcctgtt ggtttaaaat atatcgacca catggtaggc aatgtgggtt
     4441 ggaacgaaat gaatacttgg gtaaaatggt atgaagacgt gatgggcttc gtgaatttcc
     4501 tttcgtttga cgataagcaa attaccaccg aatattctgc actgatgagc aaggtaatga
     4561 gtaatggtaa cggaagaatt aaattcccga taaacgaacc tgccgaagga aagaaaaagt
     4621 cgcaaattga ggaatactta gacttctatg aaggctcggg tgtgcaacat attgcagtgg
     4681 caaccgatga tattttaacc accgtagccg acatgcgctc cagaggtatt gagttcttga
     4741 gtacgccacc acaagcctat tacgatgccg tgccgttacg tctagaagcg caccaccatg
     4801 aattgaaaga agatattaac aagctaatga agttaggaat tatgattgat gccgatgaag
     4861 aaggttattt gctacaaatt ttcaccaaac caatagaaga ccgcccaaca ttgttttttg
     4921 aagtaattca acgaatgggt gctcgcggtt ttggtgccgg aaatttcaaa gcgctattcg
     4981 aatcgataga aagagaacaa gctttgagag gaacgttata agcgttttta gaagacacgt
     5041 tctcaaacta catacaaaaa aagcaacttg aactcaagtt gcttttttta tgggtactaa
     5101 atatagattt taatttagtg cttttaagtg agaatgaaat tataagtttg catgtctatt
     5161 tctgttaaaa ggttcataaa gtaattgaca attacggtaa ctatttgcac ctccttttga
     5221 taatggaatt atattgtcaa actctagttt ttctttgcat acccatacag ttaaaattga
     5281 aagtcatttt ggtttgaaag aaattatttg ctataaaaca ttttgattga agcttgatta
     5341 aattgagcta tttcctttat taataggttt tttggaatca aaacgcactt ttttttaagg
     5401 atgtttgttg gttagtgtca ctaattttta gaatttttaa aaaaatgatg ttctatggct
     5461 atttattaat gaaataaatt cgtttatttt tactgtaaaa aattaaatta gattacatgc
     5521 gctggaattt aaaaccaaaa ccatcttcag aaaaagtaac tcaattagcc aaggaattaa
     5581 aagtagaaga attggtagca acacttttga tacaacgtgg gatagaaaca tttgaagaag
     5641 cgaaagcctt ttttcgtccg agtttagatg atttgtatga tccttatctg atgaaagaca
     5701 tggacaaagc ggtagaacgc atcgaaaaag gcattgaaaa tctggaaaac attttggttt
     5761 ttggcgatta tgatgtcgat ggtactacgg cagtttcatt agtatcttct tatttgcgta
     5821 gcctttatcc aaacgtggca acttatattc ccgaccggta tgacgaaggt tatggaatat
     5881 cttttaaagg aattgatttc gctgaagaca atggtttttc gttaatcatt gctttggatt
     5941 gtggtataaa atcgattgat catgtggaat atgcacgtga aaaaaatatt gattttatta
     6001 tttgtgacca tcataggcca ggaaatgaat tgccaaaagc ggttgcggta cttgacccaa
     6061 aacgtgagga ttgcaattat ccctatgatg aattatgcgg atgtggtgtt gggtttaaat
     6121 tagtgcaagc tttgggacaa aatcgaaatc aaacgataaa cgatttaatg gtttatttgg
     6181 atttagtggc tacggccatt gcagcagata tagttccaat gacaggtgaa aatcggattt
     6241 tagcaaaatt tggattggaa gttattaata caaaaccacg acctggaatt aaggcactaa
     6301 ttcagaatgt aaagaaaaaa gaattgacga ttacggatgt tgttttcatt gttgctcctc
     6361 gaataaatgc agcaggaaga ataaaacatg gaaacgaagc agtcgcatta ttaacggaat
     6421 acaatataga acaagcggaa caatttgcag ctgaaattga acaacacaac aatgaaagaa
     6481 aggaattgga caagcatatt acgacagaag ccttatgtca aattgaggaa aataacgaac
     6541 aagaaaaatt cactagtgtg gtttttcaag aaaattggca caaaggtgta attgggattg
     6601 tggcctctcg attgacagaa acctattatc gcccaacggt tgtgtttaca aaaagtggag
     6661 ataaattggc agcttcggcg cgttctgtga aagattttga tatttataat gcgttggaag
     6721 cgtgtgctga acatttggaa caatttggtg gtcatgccta tgctgcagga atgacgttga
     6781 aaaaagaaaa ttataaagca ttcaaagcag cttttgaagc agaagtacaa aaaaccattc
     6841 cgcctgagtt gcttattcct gagatttcta tagatgctca aatcaatttt tcagaaatta
     6901 acccaaaatt aattcgcatt ttaaatcaat ttgaaccttt tggaccgcaa aatatgacac
     6961 ctgtttttat gactaaaaat gtagtagata cgggttatgg taaaacatta ggtttggaag
     7021 acgaacattt gaaattattt gtaaaacaag acaattcgga aggtataggg gtgattggat
     7081 ttggatttgg aaaccaattg gaattgacca aaaatcgaaa accaatagaa atttgttact
     7141 gcattgatga aaatgaattt aatggaaacg tatctttgca attgcgattg aaagatatta
     7201 agcagtataa ttgaacaaat agatttcagc attggcaaaa aaagttatga taaaaaacga
     7261 tccttacgaa gctttacgat ttactgaatt taaatttttt cttgccatgc gttttgcaat
     7321 ggtttttgct tggtcgatgc aatttgttat catcgagtgg gaagtttata aattaactaa
     7381 agacccactt tcgctaggaa ttattggctt aatggagatt attccagcta ttggaatggc
     7441 attattcgca ggacatattg tggatcaaaa cgagaaaaaa aatctattgg ttaaatgtat
     7501 tcttgggttt tcagtcatta gttttggttt gtttttaatt acggttccga gtattaccaa
     7561 agatttatcc ttgcaaacca ctttatatgt aatatatggc ttagtgtttt ttggaggatt
     7621 agttcgttcg tttattggcc caactatttt ctctttactt tcattaattg ttcctaaaaa
     7681 aacctatccc aatgcagcta cctggagcag ttcgacttgg caaatgggtt cgatgtttgg
     7741 accagccata gcaggatttt ccattagctt gtttggtgtg cattggtcga tgtgcttggt
     7801 ttttgcatgt tcactatttg cgttgttatt tttgtcaaaa atagaaaaga aacctattct
     7861 aaacccaaag attggcgaac ctatatttca aagtttggca gaaggcgtaa aatttgtctt
     7921 caaaaacaaa actattttgg gtgccatttc gttagacatg attgcagtac tgtttggagg
     7981 agcaatggcg ttattaccca tatttgcaca ggatattcta aaagtaggct cagaaggttt
     8041 cggaattctg agagccgcgc ctgcggttgg ttctgtcatt actatggtgc tttcggctta
     8101 tattacctta aatagaaaag ccgggatgaa attgttgacc gctatattta tgtttggcgt
     8161 ttgcatcatc attttcggat tgtcaacaat tttttgggtt tcggttttgg cgatgttttt
     8221 cagtggtatc tttgatggcg tttcagttgt cataagaaat acaatattgc agttgcatac
     8281 tccagacaac atgcgtggaa gagttgcttc agtaaattct attttcgtag gttcttccaa
     8341 tgagttaggt gctttcgaaa gtggcgtaac agccaaatta atgggaactg ttaccgctgt
     8401 agtttttggt ggtacgatga ctttaattac agtagttttt acaggaattg cactgccttc
     8461 gtttaggaag ctcgatttac aaaaagatgt agaagcatta gaaaaacagg aatagttagt
     8521 tttcaaacac cttcaactca aatttctctc cttcaaaaac accgtaagtg aaatagccta
     8581 tccaatcgcc aaggttaatg tattcggagt tttcgcctac ctttaaaacc attggcaaat
     8641 gacggtgacc aaaaattaag taattgtaat gtttggtttc taattttcgt ttagaatatt
     8701 gcaccaacca ttcgttttct tcgccaagaa atttcacatc cgcatcgcca gaaattaact
     8761 tgttttttac cgaaagatgt tgtgctaatt ttacgccaat atcgggatgc aaccaacgaa
     8821 acaaccactt agagaaagga tttgtgaata ctttcttcat gcgtttatag cctttatccc
     8881 ctggtccttt tccgtctcca tgaccgatta ggaatgattt accgttaaag acatactctt
     8941 gattatcgtg ataaacagga atattcaact ctttttcaaa gtaatcatgc atccacaaat
     9001 catgattgcc cacgaagaaa taaatcggaa tgccactatc gcgaatttct gctaacttgc
     9061 ctaaaacacg aacgaaacct ttagggacaa cggttttgta ttcaaaccaa aaatcaaata
     9121 aatcacccaa taaaaaaatg cattcagcat ctttcttcac ttcatcaagc caagagacaa
     9181 acttttgttc gcgaggaaaa ctcgcttctg gagtaggtgc cccaaaatgt tggtcggaag
     9241 caaaatagat ttttttgttg tgggataatt gcatagtcac aaaaatagaa aatagattgc
     9301 aatttatttc cttattgttt tctttattgg aatttaaatt ttggatttaa acgttatctg
     9361 aagcatacca ctcggcaaaa gagctttcgg tttcttgtaa tttcaacgaa tgcaacagta
     9421 tctctttcgg taaacgactt ttgatttttt cagcaaaatc gattaccatg ttctcactcg
     9481 ttggttgata atttactaaa atgacatgat gaccacgatt ttttaattca tttgccaatt
     9541 ctacatgtgg tgtgttttgg ttaaaaacgg ttgcgtgatc aaataaatca acaatttctt
     9601 cacgcacgat tttctttaaa tcggaaaaat caatcaccat cccatattta acgttattgg
     9661 aatcagtaat tggtgtacca ataactgtta cggataattt ataactatgt ccatgaacat
     9721 ttttacattt tccgtcataa ccatatagtg catgacctgt ttcaaatgaa aattgtttag
     9781 taattctgat tttgttcata gactaatttt gaattgcaaa tttagtaaat tgaaagaagt
     9841 aatggagaat ttattttttc tctttattaa attagaaaaa agagtatatt tgttctcgca
     9901 gttttgggga tgtagctcag ttggctagag tgcttgactg gcagtcaaga ggtcgtgggt
     9961 tcgagcccca tcttctccac aaaagtcgaa gttaaagttg cttcgacttt ttttttctag
    10021 tttctataat ttttattttt ttctcaatta gaataattaa aaaagaaaat ggtcgctcaa
    10081 aaaggcgacc attcttataa aatatcaatt ataaattatt gattttgttg gtatgtttga
    10141 ttttcgttta aatcggcatg acgtgcatta agcgataaat tatccgcttg aacaataaat
    10201 gccgcaaaac taatgttgtc aggattagag atatttgatg gaacaggaat agaaaaagat
    10261 tttgttattt gttgtccagc agttgtgttt tcagttattg ggtctcctaa taaatcagtt
    10321 aaattaagtc ttaatacatg attgtgttca taattaggaa ctgggtttac attgccataa
    10381 taggaggtat aattaacttg atttcgaatc aagtggtctt cgagtaggta aactactaat
    10441 tttaagcctg aataattttg aacaaacttc atgtttactt caacattaac cattccgtta
    10501 gaaatagatg aagatatagc taaccctaac cctgaattat ttcccgttaa accttttgct
    10561 tgctgaatat tggaaggttc gggcgataac catgtgatgg ttctgtttaa tcttgattgt
    10621 ggcaattcta aagcaggtga gtccggcatt atcaaatctt ttaatggcat aaaattttca
    10681 aaatggtagg gatcatttcc attgtgtatg gcaacagtta ctactttatc tgtttgaaga
    10741 tgaacttgat caatagcata agcaactctg gtacaatttc cacaccatgt gcctgtgtaa
    10801 tcttcaatca aaacattttt ttgaaaatat cctggaactg gttcggcatc ttccgtatct
    10861 gctggaagat tttctatcac ttttgtatca ctacaagaaa aaatagcaaa aaatgtaagt
    10921 aaataaaata aggttttaat ctttttcata atttaaaaaa atatattaat gcaaattaag
    10981 caaaaaaatt ttaattttaa acttattatt tctttatttg tagctattac ataaactttt
    11041 ataatgaaaa atttagttac actgttttta atttttgctg ggataatagc ttattcacag
    11101 acaaaaatgc ctgatgttag tttgtcggac atagatggaa aaaaagtttc cataaaaaat
    11161 gattttaatg aaaaagacaa aatttatgtg ttttcttttt gggctacttg gtgtactcct
    11221 tgcatcaacg aattggatga aatgaatgac gttaaaggcg aatggacaaa aaatttgaat
    11281 atagagatta tcgctgttgc tacggatgat tccagaacac agaaaagaat taaaccatta
    11341 gtaaatggta aaggttggga ttataaaatt ctattagata gtaatcaaga tttaaaaaga
    11401 gcactttcaa ttgtaaacat tccttataca gttgtggtta aaaacggaga aattgttcac
    11461 atccaaaacg gatatgttcc tggaagcgag gatgaattat atacaaaatt gaagacgctc
    11521 taaaagatga agaaaattat ttttttactt ttttttggca caacattttt tgtaaatggt
    11581 caagataaag gaactgtttt tggaggtttt gaaacaaatg cacagtggta tttgaatgat
    11641 aaaacacttt atgatgaatt ttataattct gttagtcaac ctgaaaatcc tgttcgttca
    11701 aatagttatt tgtttgtaaa ttataaaatt aaaaattggt cttttggagt gcaaggagaa
    11761 gcatatgaac aagatgcttt gttaaactat aacccaaaat acaaaggagt aaatttagcg
    11821 acttattacg ctcgatttaa aatggagaaa attgatgtta cagcagggta tttttacgaa
    11881 caatttggaa gtggtttgtt gctaagaaca tgggaagata gagcattagg gatcaacaat
    11941 gccttgcgtg gaggaagaat tatttttaag ccagtttcct cctttacatt aaaagcagtt
    12001 tatggtcagc aaagaaccgg gtttgatgtt tccaatggga aaatctatgg tgtggatgca
    12061 gatatttcac tttccgacct ttttaaattt gaaaattctg atttaggaat tggtttttct
    12121 tatgtaggta gagatgaaaa aacagatatt gttaacccca agtttaatga tgtaacaaat
    12181 ggttttgcag gtagaattaa ttattctcac ggatcgtttt atctttccac ggaatatgat
    12241 tataagtcgg aggacgctgt agttcaggta agaaatcaaa ttgacaacaa tcttattaag
    12301 cctggaagtg ctttattatt caatatgggg tattctaaaa gaggattggg tattgatggt
    12361 acttttagaa gattagaaaa catgagtttc ttttcggaaa gaattgccaa aggaaactct
    12421 tttaatgact taattatgaa ttatattcct agtttaacaa aacaacatca ttataattta
    12481 gcaaatattt atgtgtatca agcacaaccc aatgttattc ttaatgacgt ttctatagta
    12541 aaggcaggag aaattggcgg tcaaatagat ttgttttata actttaaaaa aggttctaaa
    12601 ctgggtggaa aatatggtac taaagtagct gtaaattact ctcaatataa cgctttaggg
    12661 ggtaagtttt acatttatac acctcaagat taccaaacag atttttttgg gtttggaaaa
    12721 aaatattttt cggatgtaaa tgttgaaata actaaaaaat ggaataataa acttcaaacc
    12781 gtacttagtt atataaatca atactataac aaaaaattag ttgaagagac ttatggtgaa
    12841 gtgaaaacta atattatagg tgcagaagca acttatagat ttaatgacac gaaatcttta
    12901 cgtttacaag gagagcatat gtgggcagat agcgacaaga aaaattgggc tgcattaact
    12961 gcagaagtta acttaggaac taaatacacc atttttgcct cagatatgta taattatgga
    13021 aatgatgacc caaaacttag aataaattat tatactattg gaaattcata tcgatacaaa
    13081 tctactagag tttcgctttc ttatggaaga caaagaggag ggcttgtttg tgttggagga
    13141 gtttgtaggt atgttccaga aagcacaggg gtttcattgt cgttgaatac aagtttttaa
    13201 aagcaaaaga gttattgtta atactattaa taaattgggc taaggaatga tttttcttag
    13261 cccaattttt atgatacaag gatttggtta acccatttga tccattaaag ataaagtgaa
    13321 ataaatcgcg tttccttcac tttttataat ttaaaaaagc ctcatgaatt atttttcatg
    13381 aggcttgttt ttttgtgaag aggatatcta cactactgtt taataacttt tacacttgca
    13441 tttttgccat cttgagttgt aaagttcaaa acatatacag ctttatttag attagaaaca
    13501 tttattgtgt tgtaaccaga aacaaagttt atgttgttta caagtttccc gtttaagtca
    13561 taaagttgtg ctgttccaat tgatgtagca tcaaattcaa tttgagattt cactaatgta
    13621 gatgaaatat tgattccagc attgtttaag ctgctaaaac ctgaggtgtt taaattagga
    13681 ttatatctgt aagtaagggt tattgaagaa ccagtttccc ctccaaattg atttaccatg
    13741 taaaatttca aaacatattc aacaatgcta ttaccagttc ctgcgtcaaa attaacaaag
    13801 tggtcaaaat taccattttg tccattggca ggtacagttg aaactccatt tgaaggatat
    13861 gaattcccca cacttattgt gcccacacaa atcgggtcaa tacaaaactg aagattggtt
    13921 cctgtagcac cgccagttat acttatgcat ttcattttta cagaaatact gctatttgag
    13981 ctattgtaaa ttttaaagcc taaataagct gggtctgaag atgaaatgtc atttgggtca
    14041 cccaaaacag agtaagcaat aacatcgcca tcgtttaaag gagttccatc taattttttc
    14101 atggtcatct gtgcaaatga catggcagaa attaaacaga caagtgatac aataattttt
    14161 ttcataattt cttgttattt aattaataac tgtttgctga atgagctatt ttctgatgca
    14221 atgtttacaa tatattcacc tcttgaaata cctttaacag ctatagttaa ttcttgattt
    14281 tcaattactt cttttttgtt ggtttggatt aatcttcctg acatatcata aatattgata
    14341 gtatagtcac cactttttaa attactaata ttgaagaaat cacttgttgg atttggataa
    14401 atatcaaatg aaggtgcaat attgttttgt gaaactgcca gagtagcaag ttctgctgat
    14461 tttgcattaa tgatttcacc attattttga tcaatcaata ttacaattgc atgcatattg
    14521 tttatattac ttgtagcagg tactgtataa ttgaaagtat aagtaattgt ttgaccatct
    14581 gtaattgtag ttggaaccga gccatcttga cctgaatatc ctcctaacaa agctcttcca
    14641 acatgattgt aaaccatttg atttgcagga acagggttag gtaaagtttc ataacctccc
    14701 ataacaccat ttgcgccacc agcataataa tttgtttgct tataaccatt agcagtacca
    14761 gttacattac tttcagtgat gataacccct aatttatagt ttgcattaga aaaattagtt
    14821 ctaaaagttg cgtttgcgtt tattgttata tttgatccat ttacacttcc tgtggctact
    14881 aaactaacag gagtggttag tgtttttctt gaatttagat ttgtaaccat agtagattga
    14941 ctaacatcag cacctaagac aatacggtca acattcattc caggaaaacc tgaaaaacca
    15001 gcaccaccat catattctgg taacatcatt ggatcttcat tgtgaacagc aataccaata
    15061 aactcattag gataagttgt atccatataa ttcatagcga ttgctcctct tggacaccat
    15121 ccacaccatg ttccagtacc ttcttcaaat aatacttttt taggagaagc ttgactaaca
    15181 gtattaaagt tttttgttcc agtattgttt gacggatctg catctgcact tccattaact
    15241 tgtgtaatgc ttaacgctaa atctttctct actactgtac tatactgaac gggagttgga
    15301 tgattaactg tacctgttgc acccaccgca atgtttttgt taacagtaag aatatggtcg
    15361 ttggtaccat cgttccagtt tatagttaaa ttggtaacag gattgcttcc tgtgtttttt
    15421 acagtatagg ttattgtatt tgaagaattt aataatccaa aacggtttaa ttttgcagaa
    15481 accaattgta catcattttc taaaagcgtt ttgattttca cattatcaat tactaaatag
    15541 tattggtcaa aacaattaaa atgtctaaaa cttacatata ctgtttgacc aataaaagag
    15601 gtaagattta tcgatttagt tactaaactt ccatttgata cagttgtttc aaatacagga
    15661 gttgaagcaa caatagtagc aggatcgtta gaagtcgtta cataaactgc ataatgttca
    15721 tcaggatagt ttgtgttagt caactggtca taaaataaaa ataaatttga acccgttact
    15781 gatgttaaat caattgatgg tgatgtaatt aaattgttag gagtaattcc tacctgattt
    15841 acgtatgaaa aggacattaa agaaccgctt ccaagcgcag aattaagact agatgcattt
    15901 aatattgacc atccttgacc atcgttgttt aggtcgttag ccgtccagcc agctaaacca
    15961 gattcgaaat cattagaata ataagtttgt gaaaacccac aaaaagcgga taatagaaaa
    16021 agtaaagtaa tttttttcat ttgttattaa ttagttgtta ataatttatt tcgtaaaaat
    16081 aatataaaat ttaatataaa taagtaaaaa ataagtcatt aaaggtttaa aatttgtttt
    16141 tttatacaaa aatccagaaa gtgattgttt ttaacaagtt ttgttcttta aaataataat
    16201 aaagtagctt ttttttaact atctgattgt aattattttt cattttttaa atcttttgct
    16261 actttttcaa gttcggcata ccaattttct ccaaagcgtc ttattaaagc ttccctaaca
    16321 aatttataaa taggtacctc caattctttt cctaaactac aagctggatt gcaaatatcc
    16381 caacggtcat aattcacagc tgcaaattct gagaaatctt ttacgcgaat aggatataaa
    16441 tggcacgaaa ctggcttttt ccattcaatt attccttggt tatacgcttg ttcgatacca
    16501 caaagtgctg tttttccatc aaaaataaca taagcgcaat ctttattgtc aatcaatggc
    16561 gtttctaaat cttgttcggt tccttttacc cacttgccca atagctcaat ggcttctatg
    16621 ccttcttttc ttagaaatgg ttttactttt ggataaattt tttccaaaat cgttgtttct
    16681 tcttccgtta atggtgcacc agcatcgccg tcaacacaac atgcgccgtg acaagccgaa
    16741 agattacaca caaaatcttt ttctaaaata tcttcggaaa cgatggtttt gcctagttgg
    16801 aacatgttag atacattttt gcttggacaa aaataaccaa attaccaatg gcatctctaa
    16861 tttccttttt gaaatgatta cttttgcaaa aaaaatagaa atgaattttg attttagaga
    16921 aataataacc gcaactatgg tgctttttgc tgtaatagac atcgttggga gcattcctat
    16981 aattattgat ttgaggtcca aatttggtca tattcaatcg gaaaaagcat cattggtttc
    17041 tgcaggaatc atgatagcgt ttctatttgt tggggaagaa attttaaaat taattggtat
    17101 tgatgtcaat tcttttgccg ttgctggttc gtttgtgctg ttttttttag ccttagaaat
    17161 gattctcggc attcgattgt atcgcgatga acaagccagt tcggcatcca ttgttcctat
    17221 tgcttttcct ttaattgctg gtgccggaac aatgactact ttgctttcac ttcgtgccga
    17281 atttcagcca ataaatatta ttgttgccat tcttttaaac attatattag tctatgtaat
    17341 tttaaagtca tcatcaaaaa tcgaaagaat gctcagtaaa aacggtttaa gcatcattag
    17401 aaaagttttt ggtgtagtac ttttggcaat tgctgttaaa ttatttgcga ctaatgttaa
    17461 aggtctgttt atctaatact aattttttta cttttgttcc ataatattga ttaaatgaaa
    17521 ttatttacaa acatattaat tgttcttgca ttgggattaa taattttcaa cattactttg
    17581 ttggacttcc aaaatccttt taaaggcgat agtgctgtgg ctttaattgg aattgcagct
    17641 gctttttgtg cggttttaat tcttctaatt tttagaatgt ctaaaaaaat tgaagaaaaa
    17701 atgaaaaact aatttttttg gaatttatat atactctatg tttgatgttc taattattgg
    17761 tggcggtgtt tcgagcatgt cttgtgctta tattttgggt tctggttttc aaaaaccatt
    17821 tgcttcggat aaaaaaattg gaattattgc gcatcaaaaa acatcgtcac ttcaaaacgc
    17881 tatttttaat aatgcctacg gaattccttc agggaaatta ggtgccgaat tattaatcga
    17941 aagcattcaa catttacaag aaaattatcc gcatgtactt caaattaagg atgaaaaagt
    18001 tttaaaaata gaaggtattc ctggagattt tatcgtaact actaacaaac aaaattatgc
    18061 tgctaaaata attgttctcg gaattggtgc ttccaatcca tttacaatag aaggtttagc
    18121 gcagtatatt attcctcata aaaaagcaaa tccagagaaa aataaaattc aattgcaaaa
    18181 cgatgaccat ttagtaacta ctggtatata cgcttgtggc actttagccg gacacagaag
    18241 tcaattaact atcgctgcag gaagtggtgc agcagtggct acagatattt tatcactttg
    18301 gaatgacggt attcatgtcc aagtgcatga cagtgttggt aagtaaaata tgaaaatgat
    18361 tttaaataag attaggcata aacatctgca actgaaacta gatatttagt aattttattt
    18421 aaaaaaagta tagttttttc ttctgtagaa acattttcat tgttttttcc tttgtttacg
    18481 catgataaaa gccaccacaa tttatagttc aatttccgtt ttatgataga tttaatttct
    18541 tattaacatc cacggttcta actatccact ttcctgtccc aacaaggttc tgaaaacctg
    18601 ttaggattta ctgaaattct attttactac ttcaatccat tgaccttttg ttcttgctaa
    18661 aaacgtatta tcatacatgg cttcgcattg taaacctggc aaataatagt ttcctaaata
    18721 ggatgcattt aaaagtattt taaacttacg cgtttcataa gcttttaaac caaaataata
    18781 gttggctcta tcatcacgaa tgtcaatgta atcggcatta ttggtagttg cttcaccaaa
    18841 ttccgtgtaa cgtgtattta caatttcaaa accagaaggc aaaatttgcg ttaatgcaat
    18901 gttgtcaact cgttcatttt tttgattata aatcgtaacc tcagctataa attctgtgcc
    18961 ttgttttatt ttagaaacag aaactgaatt cccttttcta tctacaaaag caacactagc
    19021 tttcacatta ttttgcatca cttgctcttt gccaacagga agaattccac tattcaaaac
    19081 tctaacgaac aacgtattat tctttttgtt tttaatagtt accgaatttt tggacgatgt
    19141 aattactaaa tcacgttgag aaatcgtttt ttgggtatta attcctactg tttttcctcc
    19201 attggtatat tgcgcaataa ttccttttgt accattattc agagcaaatt ttgacatagc
    19261 aaatagtcca taagcagtag tttgcgtact catccattga ttgctcgaca aactttttgc
    19321 caatttagtt gccatttcaa aggctttttg tttttgacct aataaaatca aagtttccaa
    19381 tgccatcgct ctatttcgtt ctcttgaacc ataataggaa taataatatt cgtcagtttg
    19441 gtcatcaatg aatgatttac ttaacaacgc caaaccagct gagttttgtt taattaaagc
    19501 gtaagttgct gccaaacgta atttactttc gttagaaatt ccaactgttt ctcgcaatct
    19561 attcatcgat gctaaatcgg gtgaacccgc aactgccaac gtatataatc gataggattg
    19621 tgccaaatca ttaccatatt gcttgtcaaa acgccattgt tttgccattc gttgttggta
    19681 ggaaatccat ttgtttttaa aattaattgg catcacatag ccttttttct ctgcttctat
    19741 caagaaatgt ccggcatann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
    19861 tactttttcg gatggtgaaa tttttctagg caacgatgct aataccatta acggacttcg
    19921 aacagctgtt gttttttcag ctttaccgta tgcgcttgtt tttgcatctg cagcaacaac
    19981 catcgttcgc actgaaccaa tatattttgg aattttaatt ttagttgttt tcgtttcacc
    20041 tttacctaat tcaaatggtc caaaatagag tacaaccggt tgaaaacggt ttgctttttt
    20101 tgcttttccg ccacctaaat cagcatcacc accaatgctg aatacttgat ttacttttcc
    20161 gccataagcg ccaatcacat catcaaaaat atcccaagta cgaacaccta aagcttcacg
    20221 tgtgtaaaaa ttatcccaag catttggcgt tttaaaacgg gttaaatcca acaaaccatc
    20281 atcaaccact gcaatagtat acgtcattgc ttttttattt ttctccgaaa cttttacagc
    20341 aatggtttgc tctggacgca ggacttctgg cattgaaatt tggggttcta atactgtatt
    20401 tttatcgact acttcaattg gaacgatgcc atacattcta attggcgagt cgtttttagt
    20461 ggaagcatgg ggttgtaaaa gcgaaatatt gatgtaaaca tttggtgcca tgtcgcctgt
    20521 tacgggtaac tctaccgtag tttctccttt ctttgttttt gcccaaagtg ttttgagcac
    20581 ttttgtgccg ttttctatcg aaatcaatgc tcttccacct tcacttgacg ggaaaaatat
    20641 tttggctttt tcgcctacat tgtaatttgt tttatccgtt gagaatcgca acatagtggc
    20701 cgattctcca tctgaatttc tagatttcgc actccaatag ggcgaatcta catatatttt
    20761 tgttcctgtg gcatgtccgc cattagcatc aataacacga accaaataac ttccccagtc
    20821 gttttctgga atagaaaaag caacactgcc ttttccggcg ctattggtat taattttaaa
    20881 atttttgtag gctaacgttg aatttgaagt gctataattg gacaagttat cgttggatgc
    20941 atcccaccac caacgtgatt gcaggcgata tacgcgaaca tccaaattag aaactgcttt
    21001 tggtcgtccg ttttcatcaa gcgttgcgat gtcaaaacgg ttgtttttgt ccgtttccaa
    21061 cataccatac cgatttggtt cgggagattt tattccaaca taggtaacgt atggagaata
    21121 atttgttgaa gccacatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
    21241 ggcttgcact tcggttttta aatctttagc aaccgcaccg tgaagccaca aaacctgaat
    21301 ggtattgaag ttttctttgg atgcagaaag tatcggctcg ttgaaactgt ttttgatttt
    21361 caaacgatta ggcttgatgg tctcaatttt gatgtttttg taaaatcgtg cgccaccaac
    21421 actaacgaca gcttcccaac ttcctgttgg cgcatcagca tcggttggca cagtgaattt
    21481 ataatgattt aaggtattca tcgtaccgac tttttgaaaa gtggtctttc ctcttgggtc
    21541 gtttaatttt aattttattg gatgttttga tggaatttta ttggcattat cattcaaaat
    21601 gaacgccaaa tgcaaggtgt cgcctggtcg ccaaacgccg cgttctccat aaatgtagcc
    21661 ttttaatcct ttttgaagcg tttcgccagc cacatcaaaa ttacttaccg acaaagacaa
    21721 ttcatcgtct aatttcagat aagtactgtt atcacctttg gtaactaccg caaaataagc
    21781 atatttttct aattgaaagg aagcaaaacc atcaccatcc gttttactac tagcaatttt
    21841 ttgttgttgg taattatata aatctactgt tgcttccgaa attggttcag tagttacaat
    21901 attactaaca gcgattgcaa ttgatttatc cgttccacgt tttgcaatca caccaatatc
    21961 ggaggctaaa atatttaagg caacatttgt atttctgaaa taataataat cggtgcatgg
    22021 atctagactt tcacgccatt cataatcatc ttcataataa taatcataat tgttataact
    22081 gtaattgaca tcattttcat cttcttcctc ataatcgctt tcgcgttcat taccagattc
    22141 gcttgaatca tttgagcatt tatacatgga atagctcttt ttaaatgaaa attccacacg
    22201 gtaaattgcg ccaggttcag gtttgatgat ttttgaaata tctaaagcat aggtattcca
    22261 tttgctgtaa tctaaaagat tattttgctt cagcggaatg gttttcttgg caacggcttg
    22321 gcttacttgt ttcaaatttc gtgaaccgtt taattcgttg tcttgaagaa actgcagaat
    22381 gttgtttttg tatattttat atacttttac atcaattgca ttcaaatttg ctgcttcaaa
    22441 attaattttt aagttgtttg aactcggcaa aatagttcca ctatttaaaa actttacacc
    22501 tggtttaatt tgttcgaaaa ggattttctc ggaataattg gctttcattt tatagccatc
    22561 actattttct ataccttgaa aaacttctac caatcggctt ccataaaacg atttttcagc
    22621 cgtttcttct tcttcgtaat acggttcaac aactgatgta gaatcaactg caacattatc
    22681 agaaatagta ttggaaaccg atgtgttgtc cacttttgat ttttctcctt cattattaaa
    22741 aaacactttc aaaacatttc ccatggtggt aaatttcaaa ttgtctgtgt tttctattgc
    22801 taccaaacct ttgaaatctt ggtccttttt taatggatct gaaaaattga ttaaaatgga
    22861 ttgattgttg gtgtcaccaa tatcaatctt taatactttg aattgatttt tgcccggaat
    22921 ttggaaatcg acagaacctt tttgatcgat atcaacatca tcgccatccc aagcaatatt
    22981 tattgtggta tcatcatcaa atcgtttgat gttttcgatg gtaaatttga attccaatcc
    23041 agaattcatg cctttatcaa aagtaacttt aagtttttcg ttattttgag taacatttac
    23101 taatttttgt gcgtcttcaa aagataattt atctgccgtt tttataacac catttaggta
    23161 gtaagtatct ttattatacg attgtaaatc caaagtgttt actataaaat cttgcttgat
    23221 ggtttttacc gagaaattaa aattggataa ttccttagga actttatttg tgagttttga
    23281 aagctttaga gtaatttgat acagcgtatt ttgctctaat ttttctttag gaataaaagc
    23341 aatagtattg ctcgaaagtg caactacttt tccgctaaca cttggagata tttcgaataa
    23401 atcatcatct aattcttgat tgggtttcca atcggattta ttgaacgcca attgtactct
    23461 aaaatcggaa tgaaccgaga cataacccga tgaaaaacct gtaatgtatt ctttgaaaag
    23521 agcgaaatcg gaatcaaaat cagcagctga ttttttggaa caagattgcg acaaaactag
    23581 tgcaatcaac aagcagaaca ctttgcaatt tttcatggtt aaaaaagaga attattggtt
    23641 agaacttatt ttttgagaac gattgctcaa ttaaatatat aacgttaaaa atagcaaaaa
    23701 agtataggga aaagataata tttttagaaa aaaatctgtc taaaattatt tcctcctgga
    23761 tgcaaaaaat tttatcatta attcagaagc ttgttctgcc aaaactcctt gttttacttc
    23821 tgtttttggg tgcaatttag ttcctaaagt gaggaaacca cgttgttcat ctcgagcacc
    23881 aaaaacaatt ttagaaatct gactccaata taaagcacca gcacacattt ggcaaggttc
    23941 gagtgtgaca taaagtgtgc aacctttcaa atattttccg ccaatgaaat ttgcagccga
    24001 agttatggct tgcatttcgg catgtgccgt aacatcattt agtaattcgg ttaaattatg
    24061 cgttcgtgca ataattttat tatcgattac aatcacagca ccaacaggaa tttcaccttt
    24121 gtcaaaagcg atttctgctt cttgcaacgc ttttttcata aaatattcgt ctgtaaaagg
    24181 attttccata aaacaaaaat acaatttcaa ttcgtacatt tgcttcatgt ctaaaaaatt
    24241 acttgattcc atcaacaaac catccgattt acgaaaattg gaaactgctc aattgcctca
    24301 attagcaaag gaattgcgcg attttataat tgatattgtt tcggtaaaag aaggtcatct
    24361 tggtgcgagt ttaggtgttg tagaactgac cattgcttta cattatgttt ttaatactcc
    24421 aaatgattta ttggtttggg atgttggtca tcaagcatac ggccataaaa ttttaaccga
    24481 aagaaaaaat aaatttcata ccaatcgcca attaggtggc atttcgggtt ttccaaagcg
    24541 tggcgaaagt gtttatgata cctttggcgt tggccattct tcaacatcta tttctgctgc
    24601 tttaggaatg gcgattgctt cacaactgaa aggcgaaaaa agaaatcata ttgcggttat
    24661 cggtgatgct tcgattgcca gcggaatggc gtttgaaggt ttgaatcatg ctggtgttac
    24721 agatgctaat ttattggtga ttttaaacga caatgctatc ggaattgacc caagtgttgg
    24781 cgctttgaaa aattatttaa cttcggtaaa agaaggcaaa aatccgaaac agaataacat
    24841 gattaaatca ttgaattttg attattctgg tccaattgat ggtcatgata ttactgcttt
    24901 aatttcagaa ttggaacggt tgaaaaaagt aaaaggtcca aaatttttac atatcattac
    24961 caccaaagga aaaggattgc aacaagccga aaaagaccaa gtaaaatatc acgcgccagg
    25021 aaaatttgat gctgcaactg gagaaattct tccaaaatcg gaagaaaatc tgccgatgaa
    25081 atttcaagat gtttttgggt taacactagt tgaattagcc aaaaaaaata aaaacatcat
    25141 cggaataact ccagcgatgc caagcggaag ttcgatgaag tttatgatgg atgcttttcc
    25201 agatagagct tttgatgttg gcattgccga acaacacgcc gtgactcttt cggcaggaat
    25261 ggcaacgcaa ggtatgattg ttttttgtaa tatctattcc acttttttac aacgtgctta
    25321 tgaccaaatt attcacgatg tagcattaca aaatcttcct gtaatattct gtttagatag
    25381 agctggaatt gttggcgaag atggcgcaac acatcatggt gtttttgatt tggcgtattt
    25441 gagatgcatt ccgaatatga ttatttatgc accaaaagac gaattagatt tacaaaatat
    25501 actttacaca gcttcattag gattaaatca tccaatagcc attcgttatc cacgaggtag
    25561 aggaaaaaat ccgaatttta atatctcaaa ttccaaattc caaacgattg aaattggcaa
    25621 agctcatttg ttgaaaaaag gaacaaaagt tgccgtatta tcaacgggtt ttattggtaa
    25681 taatgtttct aaagcactag agagtattca aaacaaaaac gattttgccc attatgattt
    25741 tagttttgta aaacctttag acgaaatgct tttacatgaa atttttggaa aacacacctc
    25801 gattattact attgaagatg gcgttatcaa aggtggtttt ggttctgctg tttgtgaatt
    25861 tgcagttgca aataactata caaattccgt caaagtactt ggaattccag atgcttttat
    25921 tgaacatgga actattgatg aactgcagca aatttgtaaa attgacgtta aaagtttgga
    25981 aatacttttt tctaaatatt gaaaaaccga tttttgctca aaatttaaag tcattcagta
    26041 atgaaatttt tcaatttacg tttagttctt tttcttttat tcattgcaac tacttctttt
    26101 tctcaaaacc aaaatgtaac caaaaaagat tctctttggg aacgaaaaaa cactgtagga
    26161 ttggatattt ctcaaattgc ttttgtaaac tggaatgctg gtggagttag ttctatttct
    26221 ggactactta aaggtgattt tagtcgaaaa tacaaacgta aaaatctaaa atgggtaaat
    26281 gaattaatcg ttcgttatgg tgtaaacaaa caagatggca ttgaactaag aaaaacagat
    26341 gatgcaataa aattcaactc tacaattggt catagaaaag acagtttgtc tctttggtat
    26401 tctactgcaa aatttgagtt tagcacccaa tttacaaatg gatattccta tccgaatact
    26461 acaaatgcca tttcaaaacc ttttgcacca gcttatattt ttctaggtgt tggttctgaa
    26521 tacaatgata aaaagaataa cttgacggtt tatgtttctc cattaacaat gaaatctacc
    26581 ttagttttag accaaagatt agccaatcaa ggtgcgtttg gagttacaaa agcaacctat
    26641 gatgttgatg gaaatttact taccgaaggc gaactgtatc gctttgaatt gggttttttg
    26701 gttacaagtc aatttaaaaa ggaactctat aaaaatgtga tttttgaaaa tcgattaagt
    26761 ttatattctg actacattaa taagtttgga aacgttgatg tagattggca atttaaattt
    26821 gatttagttg tgaatgaata tattaaagcc aatatcggta ctcatatcat ttatgacgat
    26881 gacattaaag ctaaagaaga agtaaatggc gaacaagtta ccattggtcc aaaaattcaa
    26941 ttaaaacaaa tgcttggtgt aggattggtt tataattttt aagccgtaat aattctgttc
    27001 aattctaaag cttttccatc cgttaacaac gaaatataat gacaaatatg aagcaatcgc
    27061 tcataaagat tatctttctc gagatgatgc ttttcaggaa gaatttttaa caataatttg
    27121 tcgtaattgg ttgtttttcc ttcgtgatta ttattaaaag ccgagataaa tttatccaat
    27181 aaattattga tgatttgata gcccgaaagc tctttttcaa tcacttctct gctttgataa
    27241 atgtttttta cgctcaattt aataatatca tccatttgtg ctttgtactt gcttttatca
    27301 gtcaatgcaa agtgaaattt accttgtaaa atagcttctt cattttctag aaaaacacgg
    27361 actgcatcgt taattaaatt tccaattgcc aaagcacgca aataactaat tcggtcttct
    27421 ttggttgtta aggtttgata tttcttagta tcaatggtgt cttttaccag tttaattaaa
    27481 tattctaaag cataatcttc ggaaactaaa cccagattaa ttccatcttc aaaatcgata
    27541 atcgtgtaac aaatatcatc ggcagcttcg actaaaaatg ccaacggatg acgttca
//
LOCUS       DAOOBH010000020        27306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_2317_length_27306_cov_5.154123, whole genome shotgun sequence.
ACCESSION   DAOOBH010000020 DAOOBH010000000
VERSION     DAOOBH010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 27306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27306
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2317_length_27306_cov_5.154123"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..270)
                     /locus_tag="PK218_03905"
     CDS             complement(133..270)
                     /locus_tag="PK218_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HPW97685.1"
                     /translation="MYKIIAKINKLLLPSFTKRNLDLSKAKKWQLAIIAWRFYITKKA
                     L"
     gene            complement(349..2115)
                     /locus_tag="PK218_03910"
     CDS             complement(349..2115)
                     /locus_tag="PK218_03910"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HPW97686.1"
                     /translation="METILLYVLKASGLMVAFYITYILLLRNETFFTSNRWFLITGLL
                     TSLLLPIYFITKVVFIEKPKINFQNLNDVTTATIETINENSVKETFDWTQLIWIGYLF
                     VVLFLLMKMFLSFISLYKMISKGQVIDSKSYKLIYVNENSAPFSFFNTIVINPKLYSE
                     EEFESIICHEKIHCQEKHSIDVLVTKLICIFFWFNPFIWIYNKALLQNLEFIADQKAT
                     QLLENKNKYQKTLLKVVATQNCISITNHFNKSLIKKRIVMLNTNPSQKINSWKYFSML
                     PLLVAFVFFFQVKVQAQVKGENKIQAKEVKKVDKTKSNPNDTENTLNLVIDKISSDID
                     LKGDSAALKERFNIDLKVNYIKRNAKGEIIELKLSYNDNKGKKGKTEQIRTIPIRPIF
                     MKVYRKENGENEIGFYDNHEMKTLPKNDEKEKKITFIENLKDDAVIYVDDQFLMRKED
                     VQELDVNSLVSMKVLKDAKSLEKYNVIGKNEVVVIETNWTTKKPKTEDAKINYVIFTN
                     DNGDELTYSFELNYFKVPQFPSVKITENSPILILNGVQQSNPLKTIDNIDFKKIKSVR
                     VSNSKDEVAIGTPIEKIVITTN"
     gene            complement(2117..2476)
                     /locus_tag="PK218_03915"
     CDS             complement(2117..2476)
                     /locus_tag="PK218_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPW97687.1"
                     /translation="MQKLTNKEEEIMQILWKLKKAFVKEVLAEIEDDKPHYNTLSTII
                     RNLEEKGFVAYNAFGNTHQYYPIVKMEDYRKRFMNTAIETYFNNSYKSMVSFFAEEEK
                     ISADELREILDIIEKKQ"
     gene            complement(2725..3945)
                     /locus_tag="PK218_03920"
     CDS             complement(2725..3945)
                     /locus_tag="PK218_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW97688.1"
                     /translation="MVKAAFFRYIENYKGFSREIWILTLVTFINRAGTMVLPFLSKYL
                     KEDLHFSYSQVGWIMVCFGLGSMLGSWLGGKLSDKIGFYRIMIFSLFTSGLMFFGLQF
                     ITSFEGLCIAMFLIMVVADMFRPAMFVSLGAYAKPENRTRALTLVRLAINLGFAAGPA
                     LGGLIIMNIGYQGLFWADGGTCILAIIIFAILVKEKKKSKFIDKKDPDNVLTHSVFKD
                     KPFWIFLGTCLITGILFFQLFTTIPLYHKEQFNLTELQTGLLLTLNGILVFFLEMPIV
                     SYIERNKINKVKIVCLGCFAMAISLFLMLINFWSGILIIMMLFMTLAEMFAFPFSNSF
                     AMSRAPKGHEGRYMAIFTMSFSLAHILSAKVGMEIIANWGYQANWLFMGLLGLLGTGM
                     CYWVLKLTNAEKKY"
     gene            4031..4630
                     /locus_tag="PK218_03925"
     CDS             4031..4630
                     /locus_tag="PK218_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HPW97689.1"
                     /translation="MKNLFTLFLVFSIGILSAQEKKSEASKEFQDNLNKEYADVNESP
                     LTKEDLAVFKGLDFFPIDEKYIVLATFIKSKNEKIFQMKTSTTRLPQYKKYGELHFEL
                     EGKSFVLNVYQNIELIKKKDYEDYLFLPFSDVTNGKESYIGGRYIDMRIQKENIWTID
                     FNKAYNPYCAYNHNYSCPIVPLENDLEIAINAGVKKFHE"
     gene            4630..5283
                     /locus_tag="PK218_03930"
     CDS             4630..5283
                     /locus_tag="PK218_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPW97690.1"
                     /translation="MKYYNLHTHTFTNNPKVVELVNQYPWEFDNTIPQYSIGIHPWHI
                     DENRLKQDLKTIEEKLALPECLALGECGLDKRIEIPIALQLEVFEHQIVLAEKFKKPL
                     LLHLVAAYDELIALNKRLKISVPVVLHGFSKNEQVAKQLLDNGFYLSFGKYLLRNPEL
                     KTVFQSIPNDRFFLETDMIEETLEEVYVLAANYKGIDLEEMKTVVATNWKNVFKNTF"
     gene            5371..6717
                     /gene="purB"
                     /locus_tag="PK218_03935"
     CDS             5371..6717
                     /gene="purB"
                     /locus_tag="PK218_03935"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPW97691.1"
                     /translation="MQEITSLNAISPIDGRYRKKTVSLAPFFSEEALIKYRVLVEIEY
                     FIALCEADLPQLNGIESTAFDSLRKIYTEFSTDDALWIKETEKVTNHDVKAVEYFIKE
                     KFEILGLSKYKEFIHFGLTSQDINNTSIPLSTKEAFESVYMPSLIGVIAKLKELSIEW
                     KDIPMLARTHGQPASPTRLGKEILVFVERLEEQMRQLFNIPFAAKFGGATGNYNAHHV
                     AYPNIDWKKFGTQFVEETLGLHHSFPTTQIEHYDHFAAFFDALKRINTIIIDLDRDIW
                     TYVSMEYFKQKIKAGEIGSSAMPHKVNPIDFENSEGNLGIANAIFEHLSAKLPISRLQ
                     RDLTDSTVLRNIGVPIGHTIIAFDATLKGLNKLLINESKFAEDLEKNWAVVAEAIQTI
                     LRREGYPNPYEALKGLTRTNSSITQESIHQFISTLEVSDEIKAELLKITPSNYLGI"
     gene            6821..9055
                     /locus_tag="PK218_03940"
     CDS             6821..9055
                     /locus_tag="PK218_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPW97692.1"
                     /translation="MPIFLAASVVHLPNLISDLGLILVTAAIAVLIFRVLKQPLVLGY
                     LLAGFLAGSQSDFFPTVKDMNSVSVWAEIGVIFLLFSLGLEFSFKKLMKVGGTASITA
                     LTQIIGMVALGYLVGHWMNWGKMNSLFLGVILSISSTTIIIKTFDELGVKAQKFAGNV
                     IGALIVQDIMAILMMVLLSTVAVSQQFSGIELLQSVLKLIFFLTIWFVVGIFFIPTLL
                     KKAKHLLTDEMLLIVSLALCLLMVLFAANVGFSPALGAFIMGSIIAETTQAEHIEHLV
                     KPVKDLFGAVFFVSVGMLIDPEMLVKYAIPVGILTFVVIIGQSFLSTVGTLLSGQPLK
                     QSVQTGMSLSQIGEFSFIIATLGITLKVTSDFLYPIIVAVSAITTFTTPFMVKLSTPF
                     SDYLVKHLPKRWTKRIERYSANTQAIRSESNWRIVLRAYLVQVIIHSTIIVSIILISS
                     NYLIPMLPESKFAHIIVAFITLIIISPFLYALSLRRVAIVQVNELLKERKYRGPIIMM
                     VFLRALLTLVFLGFFLNVFFSPVIAFIALIICLVVFWLFPKKLTARYYKIEERFIKNL
                     NSRELAKAKRSRSDLTPWDGHMATFEIARESNISGKTLEELKLRENLGINVAYIKRGD
                     IIINIPSKNERIFPADEIGVIGTDNQVKELKIFLDQNEAEEKPDYKEPDIVLRQINLK
                     NQSYIGKSIRESNLREKTSGLVVGIERNGNRFLNPESTLILLDGDILWIVGNKKLLNE
                     LTHN"
     gene            complement(9103..10575)
                     /gene="guaB"
                     /locus_tag="PK218_03945"
     CDS             complement(9103..10575)
                     /gene="guaB"
                     /locus_tag="PK218_03945"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPW97693.1"
                     /translation="MNAHTSKIVGEGLTYDDVLLIPSYSEVLPREVTIQSKFSKNITL
                     NVPVVSAAMDTVTESAMAIAMAQEGGIGVLHKNMTIEQQAAKVRKVKRAESGMIIDPV
                     TLPLTAKVIDAKNAMKEFGIGGIPIVDKNQILKGIVTNRDLRFEKNLERPIVEVMTKK
                     KLVTVAEGTSLQQAEVVLQGHKIEKLPVVNKDYKLVGLITFRDITKLTQKPIANKDTF
                     GRLRVAAAIGVTGDAIDRATALYNAGIDAIVIDTAHGHTKGVVAILKEVKTKFPDLDV
                     VVGNIATAAAAKYLVENGADAVKVGIGPGSICTTRVVAGVGFPQFSAVLEVAAALKGT
                     GIPVIADGGVRYTGDIPKAIAAGASSVMLGSLLAGTKESPGETIIFEGRKFKSYRGMG
                     SVEAMQEGSKDRYFQDVEDDIKKLVPEGIVGRVPYKGELNESMQQFIGGLRAGMGYCG
                     AKDIPTLQETGQFIRITNSGIKESHPHNVTITKEAPNYSR"
     gene            complement(10674..11537)
                     /locus_tag="PK218_03950"
     CDS             complement(10674..11537)
                     /locus_tag="PK218_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPW97694.1"
                     /translation="MKKVKIIECPRDAMQGIKEFIPTEKKVAYIQSLLRVGFDTIDFG
                     SFVSPKAIPQMADTAEVLAQLDLSETESKLLAIIANTKGAELAAAHSEIQYLGFPFSI
                     SENFQMRNTHKTIAESLVTLQEILDIAKSTNKEVVAYLSMGFGNPYGDIWNVDIVAQW
                     TEKLAKMGVKILSLSDTIGSSTPDIIDYLYSNLIPKYPDIEFGAHLHTTPDKWHEKVA
                     AAYNAGCTRFDGAIQGFGGCPMAKDDLTGNMPTEKLLSYFTAEKVATNCSPMSFESSY
                     NEALKIFTKYH"
     gene            11780..12388
                     /locus_tag="PK218_03955"
     CDS             11780..12388
                     /locus_tag="PK218_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HPW97695.1"
                     /translation="MHSIITGDIIGSRKSKSIDWIDGLKVLLSKFGSTPKNWEIYRGD
                     EFQLEINDASQAIAIALQIKAFLKSIKLDARMSIGIGSKTYTAQSISESNGSAFVFSG
                     EGFNRLKKNKINLSVNSEYKEFNTEINLMLRLGLTFMDNWTAQLAEYVLVAMQNPDLS
                     QEEIGQKLGINQAAVSRRRKRANFDVMLESIVFYIEKTKQLK"
     gene            12388..13095
                     /locus_tag="PK218_03960"
     CDS             12388..13095
                     /locus_tag="PK218_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HPW97696.1"
                     /translation="MVVFVKLFLAHILGDFILQPSKWVEDKEQKRGKSFYLYTHTFLH
                     FLLTIILVAKTDFIVYGLILAIFHGGIDYAKLTFQKTKTKRNWFLVDQVLHVISIVII
                     GSIYLNLKFDYSFLNTNFWVIVTAILLITKPTSIIIKNIISIWTPENKDKKDNSLQNA
                     GNFIGILERLFVFYFVLTNHIEAIGFLLAAKSIFRFGDLKEAKDRKLTEYVLIGTLLS
                     FGIAFLLAIVVNYLTIL"
     gene            complement(13081..14100)
                     /locus_tag="PK218_03965"
     CDS             complement(13081..14100)
                     /locus_tag="PK218_03965"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HPW97697.1"
                     /translation="MYKLLIRPILFLFDPEKVHYFTFSLIRFLTKLPGFSSLFKSMYV
                     IKDKRLETEVFGLKFPNRVGLAAGFDKNALLYKELSNFGFGFIEIGTVTPKPQDGNPK
                     KRLFRLKEDAAIINRMGFNNDGVDEAVLHLKKNKTILIGGNIGKNKVTSNENAVDDYI
                     ICFEKLYAYVDYFVVNVSSPNTPNLRELQEKEPLTKLLQVLQNLNAEKQVPKPILLKI
                     APDLTNEQLLDIIAIVNETQIAGVIATNTTISRESLTSNNKVEIGGLSGKPLTNRSTE
                     VIRFLSEKSNKAFPIIGVGGIHCAQDALDKLNAGASLVQLYTGFIYEGPKLVKEINKA
                     ILKNS"
     gene            14233..15285
                     /locus_tag="PK218_03970"
     CDS             14233..15285
                     /locus_tag="PK218_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW97698.1"
                     /translation="MNLLNSNLSKVTLLIISLTFLLLVGIVDYYTGSEISFLLFYLIP
                     VFFLSLHRNSNKRLIIINSIIGGVIWFLSEYYSRNYTNDLIPVWNALVRLFVFLLFGM
                     LLRLLNERFLKLEVVNKQLQLLNIEKNKFIGIAAHDIKNPIGTISSFSDLLISDFSDK
                     MDDEAIEMIGYIKELSSNSLHILKNVLDISKIESGIIEIKTNKQDYIQFIKKQIFYYQ
                     ILAKKKDIKLIFESELEKFIFEFDENYLGEVISNLLTNAIKFSNKNAEIIIKVSSNRD
                     KKLKTEIIDFGKGIPESEHYKLFNYFQKTTTQPTDGEQSTGLGLAISKKIITEHKGII
                     NFTSEVNKGSNFYFEL"
     gene            15363..16220
                     /gene="nadC"
                     /locus_tag="PK218_03975"
     CDS             15363..16220
                     /gene="nadC"
                     /locus_tag="PK218_03975"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPW97699.1"
                     /translation="MISEAQFQKELDLIIINGIREDIGPGDYSSLACIPKEAKGKAKL
                     LVKDKGVIAGVEFAKMIFKAVDENLKVETFINDGAKVNFGDIVFHVSGSSQSILKAER
                     LVLNSMQRMSAIATKTNHFTQLLEGTKTKVLDTRKTTPGFRACEKWAVKIGGGENHRF
                     ALYDMIMLKDNHNDFAGGISKAIQKTQTFLTKNKLDLKIIVEARTLDEIKEILQNEGV
                     FRILIDNFNFEDTKTAVALIGNKCQTESSGNINENTIRSYADCGVDYISSGALTHSVY
                     NMDLSLKAF"
     gene            16223..17167
                     /locus_tag="PK218_03980"
     CDS             16223..17167
                     /locus_tag="PK218_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPW97700.1"
                     /translation="MSVPLNQKLKKLILVRQVLYITKKLKLPWLQGLSFYDLMKIYIT
                     GIIDGAISYRAAAIAWSFFMALFPFALFILNLIPYIPIDHFQDDFLAFVQQSVPPTTY
                     DAIFTIINDILTKSNTGLISTGFFMAILLMSNGVNAVLGGFESSNHIQISSKRKFFRQ
                     YFVALALSILLSLVLIITVAAIVIFEFYIQKTKLQDVLSDDIPLIEMGRYIFVILMIL
                     ITTSLLFKFGIKREKNRAFISIGSVFTTVLIIISSYFFGIWVVKFSKYNELYGSIGTL
                     LVVMFYVWINCMILLLGFDLNAAINKIKRDKQLELELL"
     gene            17164..17577
                     /locus_tag="PK218_03985"
     CDS             17164..17577
                     /locus_tag="PK218_03985"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HPW97701.1"
                     /translation="MKLVLFFIVFSTSIFAQTIEGKWKTIDTKTQKELSIIEIYKKDN
                     QFYGKLLKISNVLSSHADCEKCSAKIKNNSLIGKNVIVKMQKQDNQYKGKLIDPFSDK
                     SYTCVIKMLSNSTLEINAYSVLPLFGRTQIWRKIN"
     gene            17593..18015
                     /locus_tag="PK218_03990"
     CDS             17593..18015
                     /locus_tag="PK218_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HPW97702.1"
                     /translation="MKKIVLVLLFYFTQLSFSQSIVGKWTTIDDETGVEKSVVEIYES
                     GGDFYGKIIEIKEVEHRNKKCTNCNGTDKDKPILGLIIIKKLSKDNDVYSGGTITDPK
                     NGKEYRCKIILDGNDKLIVRGYIGISLFGRSQTWKRKK"
     gene            18024..20474
                     /gene="priA"
                     /locus_tag="PK218_03995"
     CDS             18024..20474
                     /gene="priA"
                     /locus_tag="PK218_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPW97703.1"
                     /translation="MNYFIEVVVPLSLDKTFTYRVSEAEYLFLKKGMRVAVPFGKNKI
                     YTALVVGLHQTAPLLYEAKDIHEIIDEKPIVNEFQIKHWFWIAEYYMCSIGDVYRGAM
                     PSALLLESETIITQKSSTSIDESTFSNDEFLIYEALQHQSSLKIQDVIAILNKKTVLP
                     IIQKLIQKEVILLHEEIQEEYKPKLIKYIRLADEFLKEEKLNELLNTISSDKQKTLLL
                     TYFQLKAQESKPISTKKLVDTASISSSFLKKMIEKSIFEEFHIQEDRVNFRNNKAENE
                     LHLSQPQQIAFDEIKQVFLSKDVCLLHGITASGKTEIYIKLIEKQLESGNQVLYLLPE
                     IALTTQLVSRLTKYFGNKVAVFHSKYSNNERVEVWQNVLNSSEKAQIVIGARSALFLP
                     FSNLGFVIIDEEHEQTFKQQDPAPRYHARDAAIVLANFFKAKVVLGSATPSIESYYNA
                     TSDKFGLVSLNQRFGNFQLPEIELIDLKDSYFRKKMKGHFSLSLIDAISEALSNNEQI
                     ILFQNRRGFSPVLECLTCGHVPQCPQCDVSLTYHKFKNQLRCHYCGHSIAKPTNCHAC
                     SSIDLETKGFGTEQIELELAELFPQKIIKRMDQDTTRGKYSFEKIIDGFKNREIDILV
                     GTQMLAKGLDFDNVTLVGIMNADNMLYHPDFRAFERSFQMLTQVSGRSGRAEKKGKVL
                     IQTYSPLHNIIQQVTHTNYIGMYKEQLYERQIYNYPPFFRLIKITLKHRNFEKNKDGA
                     MWLYQVLRQNLGFPVLGPEEPVISRIRNEYIRTILVKIPTETSMQSTKKTIQKILNSF
                     DMIAHYRSIKVTVNVDFY"
     gene            complement(20471..21166)
                     /locus_tag="PK218_04000"
     CDS             complement(20471..21166)
                     /locus_tag="PK218_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPW97704.1"
                     /translation="MKLNCVVVDDSSIQRMIIAKLVNNHPNLHLVGDFSNAIEAKNCM
                     SVHTVDLIFLDIEMPVISGFDFLDGLKTKPQIIFITSKAEYAMKAFDYDATDYLQKPI
                     ALDRFNASVRRAMEFHMLKKENQEEEGEHIFIKSNLKKLKVYTNKIKWIEAYGDYVKV
                     VTEEDSNLVLSTMKAFEKDLPSEKFIRVHKSYIINIDKVERFNSKFAEIGVTKIPLSR
                     NKKEDLIKALAIA"
     gene            21355..21693
                     /gene="rpsF"
                     /locus_tag="PK218_04005"
     CDS             21355..21693
                     /gene="rpsF"
                     /locus_tag="PK218_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPW97705.1"
                     /translation="MNHYETVFILNPVLSEVQVKETVSKFEDFLTAKGAKMVSKEDWG
                     LKKLAYEIQHKKSGFYHLFEFQLAGEHLIAFETEFRRDERVMRFLTVSLDKHAVSWAE
                     RRREKLKTKA"
     gene            21699..21995
                     /gene="rpsR"
                     /locus_tag="PK218_04010"
     CDS             21699..21995
                     /gene="rpsR"
                     /locus_tag="PK218_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPW97706.1"
                     /translation="MSTIEQNAKGKKDGDIRYLTPLNIETSKTKKYCRFKKSGIKYID
                     YKDADFLLKFVNEQGKILPRRLTGTSLKYQRKVSVAVKRARHLALMPYVADLLK"
     gene            22066..22509
                     /gene="rplI"
                     /locus_tag="PK218_04015"
     CDS             22066..22509
                     /gene="rplI"
                     /locus_tag="PK218_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPW97707.1"
                     /translation="MELILKQDVQNLGFKDDVVTVKNGYGRNYLIPQGFATLATPSAK
                     KVLAENLKQRAYKEAKIVADATTLAESIKALEIKLMAKAGGEKLFGSISNIDIANALE
                     AAGHSVDRKFITSGIVKRTGKYTASVRLHREVIVELPYEIIAEQA"
     gene            22595..23068
                     /locus_tag="PK218_04020"
     CDS             22595..23068
                     /locus_tag="PK218_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6495 family protein"
                     /protein_id="HPW97708.1"
                     /translation="MKYKRLTKEQFEELHVEFSNFLATQSIDKKEWDTIKANKPEIAE
                     QELDVFSDLIWEGVLKNTHYLEHFSKNHIFLFHCQEKLIQSIVLKSLDTTIDFMNKEG
                     LQWLSDNMFTEQVEIHLGKKEYETLRNESIFKLITEGAILSDGQLYLQINGIIQS"
     gene            23138..25147
                     /gene="ligA"
                     /locus_tag="PK218_04025"
     CDS             23138..25147
                     /gene="ligA"
                     /locus_tag="PK218_04025"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPW97709.1"
                     /translation="MDIQQTISNLRDDLNQHNYNYYVLDTPTISDFEFDQKLKRLQEL
                     EAQNPEFFDENSPTQRVGGTITKNFETIVHDYRMYSLDNSYSKEDLIDWETRIQKVLG
                     NVALEYTCELKYDGASISITYENGKLIRAVTRGDGFQGDDVTNNIKTIKSIPLQLKGN
                     FPKRFDIRGEIVLPFEGFAKMNQELIEIGETPYSNPRNTASGSLKLQDSSEVAKRPLE
                     CLLYFIVGNHLGLKTQFESLEAARNWGFKVPKEAKLASNLEEVFVFIEYWDKHRHDLP
                     YETDGVVIKVNSFQHQDELGYTAKAPRWAMAYKFKAERVSTTLNSIVYQVGRTGAITP
                     VANLEPVQLAGTIVKRASLHNADQIAKLDIRVDDTVFVEKGGEIIPKIIGVDFEKRAS
                     ISKPTTYITNCPECNTKLNRVEGEANHYCPNFYGCPPQIIGRIEHYISRKAMDIDGLG
                     GETVALLFNNGLVKNYADLYELTVEQILPLDRMAQKSAENLVNGVQKSKEIPFERVLY
                     AIGIRYVGETVAKKLAKHYKTIEALQQATLMDLVLVDEIGEKIAQSVITFFENDENVT
                     TINRLKKYGVQLEIIEKTNPNATSILNGKTFVVSGVFSLFSRDELKQSIEDNGGKVGS
                     SISTKTNFVIAGENMGPAKLDKANQLKIPIISENEYVNLLKNGIE"
     gene            25134..25889
                     /locus_tag="PK218_04030"
     CDS             25134..25889
                     /locus_tag="PK218_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97710.1"
                     /translation="MELNKKKHYFNNINLIKMSYKEAKIPLHLFVVSSLVYIISNFME
                     IEIVGLLVKPIIIPSIFFYYLMTKKNNINISFSLIFILFFIGDMIIMLYSQEFMSLIL
                     APFMFSYIILLKFIFEDINFKAKSIYTIFYAVLVFVLLSLILYYILDLPVDKVINNNI
                     LFLFYGILLITLVTISTYTYLSNQNFNTLNLLLMAIAMLTSDLLYCINKFIFSFYIID
                     SLNLLAQFASYYFMVVYFNSRINTNTILLLKRE"
     gene            25890..26600
                     /locus_tag="PK218_04035"
     CDS             25890..26600
                     /locus_tag="PK218_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HPW97711.1"
                     /translation="MKRFLGKKIFRLFIIAYFTVAMIEIVAEYFNDKTLVTLFKPILI
                     PLLIGYYFYNSRKRNFLFIVALVFSWIANIFFINDTINYIILGSVFFLIYRVIIIYLV
                     IRMVKMPSKLPLLIGAIPFLFIYATTCFLTFEDFGENIFLFIIHGIFIIFLGGYSLGS
                     YIVYSSKTNLILFLSTMLFALSQFVFVLKIYSEYVYFLHALAMLQFVVAQYLLTKFMH
                     LKEKPKSKYEFANTINEL"
     gene            26597..27292
                     /locus_tag="PK218_04040"
     CDS             26597..27292
                     /locus_tag="PK218_04040"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HPW97712.1"
                     /translation="MKNIMVSKLNILKGFTILYFIISIIEITAEYLSNKAMIIALKPL
                     IPLVIIILYFIESNKKNKLYVVAMFFSILTNLLFIPSIAVFLFYGIIAFLIHRIIIIY
                     LLVKINPIKDFIPFLLATAPFLLVFFYLFIETENIPENSYAILVLQNILMSIFAGIAL
                     SNYVMNDNKQNSILLISAFLFVLLQFVIFIEKYFLIYELKEIFRPMAMLLNVFAFYTL
                     FRYIIETEKLNND"
BASE COUNT         9583 a         3938 c         4204 g         9581 t
ORIGIN      
        1 cagtaacctc ttgagccgta atcaagtgcg ctattcagtt gcgccacgag gccttttttt
       61 gtgggtgcaa atataggtat tattgtgaaa ggtgcaaatg gttttcaaaa taaaatttaa
      121 tatttattaa ttctacaatg ctttttttgt gatataaaac cgccaagcta taatggcaag
      181 ttgccacttt tttgcttttg acaaatctaa attccgtttt gtaaaactgg gtaacaagag
      241 tttattgatt ttagctatta ttttatacat aatgagttcg aattaaaaaa acgtttagtc
      301 agttaaacca atcggtttca aatttatttt ttttatctca ataaacaact aatttgtagt
      361 aatcacaatt ttttcaattg gtgttcctat agctacttca tcttttgaat ttgaaactcg
      421 caccgatttt attttcttaa agtcaatatt gtcaattgtt tttaaaggat ttgattgttg
      481 cacaccattt agaataagta taggcgaatt ttcagtgatt tttacagatg gaaattgtgg
      541 aactttaaaa taatttagtt caaaactata ggttaattca tcaccattat cattggtaaa
      601 tattacataa tttattttag catcttccgt ttttggtttt ttagtcgtcc aattcgtttc
      661 aatgacaaca acttcatttt tacctataac attgtatttt tctaaagatt ttgcatcttt
      721 taatactttc atactaacca aactatttac atctaactct tgaacatcct cttttctcat
      781 aagaaattga tcatcaacat aaataacagc atcatctttt aaattttcaa tgaatgttat
      841 ttttttttct ttttcatcat tttttggcaa cgtcttcatt tcgtggttat cgtaaaaacc
      901 aatctcattt tccccatttt cttttctata cactttcata aatattggtc gaattggaat
      961 tgttcttatt tgttccgttt tccctttttt tcctttattg tcgttatagg aaagttttag
     1021 ttcaataatt tctcctttag catttctttt tatataattt acttttaaat caatattaaa
     1081 acgttctttc aatgcagctg aatcaccttt taaatcaatg tctgaactaa ttttatcgat
     1141 tactaggttt aaagtgtttt cagtgtcatt tgggtttgat tttgttttat caactttttt
     1201 aacttcttta gcttgaattt tattttcgcc ttttacttgc gcttgaactt ttacttgaaa
     1261 aaagaaaaca aaagcaacaa gtaaaggcag catggaaaaa tatttccatg aattgatttt
     1321 ttgagatgga ttggtgttta acataacgat tcgttttttg attaatgatt tattgaaatg
     1381 attggttatt gatatgcaat tttgtgtagc tactacttta agtaatgttt tttggtactt
     1441 gtttttattt tctaataatt gtgtagcttt ttgatcggca ataaattcta aattttgaag
     1501 taaagctttg ttgtatatcc aaataaatgg gttgaaccaa aagaaaatac aaattagttt
     1561 agtaaccaaa acatcaatgg aatgcttttc ttgacaatga attttttcat ggcaaatgat
     1621 actttcaaat tcttcttcag aataaagttt aggattgatt acaatagtat taaaaaaaga
     1681 aaatggagca ctgttttcat ttacatagat tagtttatag cttttactat caattacttg
     1741 tccttttgaa atcattttat aaagtgaaat aaaactcagg aacattttca tcaataaaaa
     1801 caaaacaaca aacaggtatc caatccaaat tagttgtgtc caatcaaaag tttcttttac
     1861 agaattttca tttatagttt caattgttgc agttgtgaca tcgtttaaat tttgaaaatt
     1921 gattttaggc ttttcaataa aaactacttt tgtaataaag taaataggca atagcaaaga
     1981 agtcaataat ccagttatta aaaaccatcg attgcttgtg aaaaaagttt catttctcaa
     2041 tagtagaata taagtgatgt aaaatgcaac catcaaacca gaagctttca aaacatataa
     2101 tagtatagtt tccatcttac tgcttttttt cgatgatgtc caaaatttca cgtaattcgt
     2161 cggctgagat tttttcttct tctgcaaaaa aagacaccat gcttttgtag gaattattga
     2221 aatacgtttc gatagctgta ttcataaaac gctttctata gtcttccatt tttacaattg
     2281 gataatattg gtgcgtattt ccaaaggcgt tgtaagccac aaatcctttt tcttccagat
     2341 ttctaataat agtcgaaagt gtgttatagt gcggcttgtc gtcttcaatt tctgctaata
     2401 cttcttttac aaatgctttt ttgagcttcc ataaaatttg catgatttct tcttccttgt
     2461 tcgttaattt ttgcatgatg attgtttttg tttaatacaa caaagcaaac atacaactat
     2521 ttttttagtt actaaactat ttttatagtt aattaactaa ttttttagtt ttgaatgaaa
     2581 ttaaaaaaat caacacattg attattagtt gttaaaaaca aattttgtat gtaatagtta
     2641 aatttagtat ttggatttat tttgaagtaa aactaaaaaa taacaactgt ataaagtctt
     2701 gaaatgcgaa atcaatactt gcaactaata ttttttctcg gcgttggtta gtttcaaaac
     2761 ccaataacac attcctgttc cgagtagccc aagtaaaccc atgaaaagcc aattagcttg
     2821 ataaccccaa ttagcaatta tttccattcc aacttttgcg cttagaatat gtgctaaact
     2881 aaaactcatc gtaaaaattg ccatatatct gccttcgtga ccttttggcg cacgactcat
     2941 agcaaatgaa ttggaaaaag gaaatgcaaa catttcggct agcgtcataa ataacatcat
     3001 aatgattagt atgccggacc aaaaattgat tagcataaga aacaaactta ttgccatggc
     3061 aaaacatcct aaacaaacaa ttttgacttt gttgattttg tttcgctcaa tataactaac
     3121 gattggcatt tctagaaaga acactaaaat accatttaaa gttaatagca aacctgtttg
     3181 taattcggtt aaattgaatt gttctttgtg atagagtggg attgtagtga ataattgaaa
     3241 aaataatatt ccggtaatca gacacgttcc aaggaaaatc caaaagggtt tgtccttaaa
     3301 aacagaatgc gttaatacat tatcaggatc ttttttatca atgaatttag atttcttttt
     3361 ttcttttact aaaattgcaa aaataataat tgccaaaatg caagtaccac catcagccca
     3421 aaataaaccc tgataaccaa tattcataat gattagtcca cccaaagctg gtcctgcggc
     3481 aaatcctaag ttgattgcta aacgtactaa tgtcaaggct cgtgttctat tttctggttt
     3541 tgcataagcc ccaagcgaaa caaacatagc aggacgaaac atatcggcaa caaccattat
     3601 tagaaacata gcgatgcata aaccttcgaa gctagtaatg aattgtagtc caaaaaacat
     3661 taaaccgctc gtgaaaaggc taaaaatcat tattcgataa aaacctattt tgtctgaaag
     3721 ttttcctcca agccatgaac caagcataga gcctaaacca aaacaaacca taatccagcc
     3781 tacttgacta tacgaaaaat gcaaatcttc tttaaggtat ttggataaaa aaggcagcac
     3841 catcgttccg gctcgattaa taaatgtaac caaagtaagt atccaaattt ctctagaaaa
     3901 acctttgtaa ttttcaatat aacgaaaaaa agctgcttta accataggtt ttgattatcg
     3961 tggcaaaggt aaaactttga aacgttgtga ctttgaaagt tttcaagtat ttttgttaaa
     4021 attacttcaa atgaaaaatc tatttacatt atttttggta ttttccatag gtattctttc
     4081 ggctcaagaa aagaaaagtg aagcttcaaa agagtttcaa gacaatttga acaaagaata
     4141 tgccgatgtt aacgaaagtc ctttaaccaa agaagattta gccgtattta aaggtttaga
     4201 tttctttcca attgatgaaa aatatattgt tttggcaact tttataaaat ctaaaaatga
     4261 aaagatattt cagatgaaaa cttctactac tagattgcca caatataaaa aatatggaga
     4321 attacacttt gaattagaag gaaaatcgtt tgtgttaaat gtttatcaaa atatagaatt
     4381 aattaaaaag aaagattacg aagattattt gtttttgcca ttttcagatg taaccaatgg
     4441 aaaagaaagt tacattggcg gacgttatat tgatatgcga attcaaaaag aaaatatttg
     4501 gacaatagat tttaataaag cttacaatcc ctattgtgct tataatcata actattcttg
     4561 cccaatagtt cccttagaaa acgacttaga aattgcaata aatgctggag ttaagaaatt
     4621 tcacgaataa tgaaatacta caatctacat acacatactt ttacaaataa tcccaaagtt
     4681 gtagagcttg ttaatcaata tccttgggaa tttgacaata cgataccaca gtactccatt
     4741 ggcattcatc cttggcatat tgatgaaaac cgattaaaac aagatttaaa aaccattgaa
     4801 gaaaagttag ctttaccaga atgtctagct ttgggcgaat gtggtttaga caaacgaatt
     4861 gagattccga tagcattgca attagaagtt tttgaacatc aaattgtatt ggccgaaaaa
     4921 ttcaaaaaac cattactatt gcatctagtt gctgcctatg atgaattgat tgcactcaat
     4981 aaacgattaa aaatttctgt tcctgtcgta ttacacggtt tttcaaaaaa tgaacaagtt
     5041 gccaaacagc ttttggataa tggattttat ctttcttttg ggaaatattt attacgaaat
     5101 cctgaattga aaacagtatt tcaatcgata cccaatgatc gttttttttt agaaacagac
     5161 atgattgaag aaactcttga agaagtttat gttttagcag caaattacaa aggaattgat
     5221 ttagaagaaa tgaaaacagt agttgctaca aattggaaaa atgttttcaa aaacactttt
     5281 taaataaatt tactcaaaat atctttccgc ttttttcttt cttctaaaat ggtttaacta
     5341 tttttgcact tcaacaacac acaagacaca atgcaagaaa taacttcatt aaatgccatt
     5401 tctccaatag atggaagata tcgaaaaaag acagtaagtt tagcaccttt tttttcagaa
     5461 gaagcactta taaaatatag agttttagta gaaattgaat acttcattgc attgtgtgaa
     5521 gctgatttac ctcaattaaa tggcatcgaa tcaactgctt ttgattcctt gagaaaaatt
     5581 tatactgaat tctctactga tgatgcactt tggattaaag aaaccgaaaa agttaccaat
     5641 catgacgtaa aagctgtaga atattttatt aaagaaaaat ttgaaatctt aggtttatca
     5701 aaatacaaag agtttatcca ctttgggtta acttcacaag acattaataa tacttccata
     5761 ccactttcga ctaaagaagc ttttgaaagc gtgtatatgc cgagtttaat tggagttatt
     5821 gcaaaattaa aagaattgtc gatagagtgg aaggacattc caatgttggc tagaactcac
     5881 ggtcaacctg catcaccaac acgtttagga aaagaaattt tagtatttgt agaacgtttg
     5941 gaagaacaaa tgcgtcaatt atttaatatt ccatttgcag caaaatttgg cggagctaca
     6001 ggaaattata atgcacatca tgtagcttat ccaaacatcg attggaaaaa atttggaaca
     6061 cagtttgtcg aagaaacatt agggttacat cattcgtttc caacaaccca aatagagcat
     6121 tatgaccatt ttgcagcatt ttttgatgca ttaaaacgta ttaatacgat aataattgat
     6181 ttagatagag atatttggac gtatgtttcg atggaatact ttaaacaaaa aataaaagct
     6241 ggtgaaatag gttcttcggc aatgccacat aaagtaaatc caattgattt tgagaattcc
     6301 gaaggaaatt taggaattgc aaatgctatt tttgaacatc tttcagccaa acttcctatt
     6361 tcaagattac aacgtgattt gactgatagt acggttttga gaaatattgg agttccaatt
     6421 ggacatacta ttattgcttt tgatgctacc ttgaaaggat taaataaatt attgattaac
     6481 gaaagtaaat ttgcagaaga tttagagaaa aactgggctg ttgttgccga agctatccaa
     6541 acaattttgc gtcgtgaagg atatcctaat ccttatgaag ctttaaaagg tttgacacga
     6601 acaaattcct ctattactca agaatcaatt caccaattca ttagcacatt agaagtttca
     6661 gatgaaatta aggcagaatt attaaaaatt acacctagta attatttagg gatataatat
     6721 tttaattttt ataaatttac tgctaccaag cctattaatt attttacgaa tagtattgga
     6781 ttaactcata acgtttacta ttttagtaaa aattactttt atgccaatat ttttagctgc
     6841 ttcagtagtt catttaccta atttgattag tgacttagga ctaattctag tcactgctgc
     6901 aattgctgtc ttaatatttc gagtgcttaa acaacctctg gttttaggat atcttttagc
     6961 gggtttttta gcgggcagtc aatccgattt tttcccaacg gttaaagata tgaacagcgt
     7021 ttctgtttgg gcagagattg gtgttatttt ccttttgttc agtttgggat tggaatttag
     7081 ttttaagaaa ctaatgaaag ttggaggcac agcttcaatt accgctttaa cacagattat
     7141 tggaatggtt gcactaggct atttagttgg tcattggatg aactggggaa agatgaacag
     7201 tttgtttttg ggtgtgatat tatccatttc ttccacaaca attattataa aaacatttga
     7261 tgaactaggt gttaaagcac aaaaatttgc tggaaatgta attggagcat taatagttca
     7321 agacattatg gcaattttaa tgatggtctt gctatcaacg gttgctgtta gtcagcaatt
     7381 ttctggaatt gagttattac aatcggtctt aaagttaatt ttctttttaa caatttggtt
     7441 tgtggttgga atttttttca tcccaacatt gttaaagaaa gcaaagcatt tattgaccga
     7501 tgaaatgtta ttaatagttt cattagcatt atgtttgttg atggtattat ttgctgcaaa
     7561 tgttggtttt tctccagcgt taggtgcttt tataatgggt tcaatcattg cagaaaccac
     7621 acaagcagaa catattgaac atttagtaaa acctgtaaaa gatttatttg gagctgtttt
     7681 ttttgtatct gttggaatgc taattgatcc tgaaatgtta gtaaaatatg ctattccagt
     7741 tggaatttta acctttgttg ttattattgg acaatcattc ttgtcaactg ttggaacatt
     7801 attgtctggt caacctttaa aacaatcggt tcaaacagga atgagtttat ctcaaattgg
     7861 agagttttct tttatcattg caactttagg aattacatta aaagtcacta gtgatttttt
     7921 atacccaatt atagttgcgg tgtcagcaat aacgaccttt actacacctt ttatggtgaa
     7981 attatcaaca cctttttcag attatttagt taaacattta ccaaaacgat ggacaaaaag
     8041 aatagaacga tatagtgcaa atacacaagc cattcgttct gaaagcaatt ggcgaattgt
     8101 attaagagct taccttgtgc aagttattat tcactcgact ataatagttt ctataatttt
     8161 aatttcatca aactacttaa taccaatgtt acctgagtca aagtttgctc atattatagt
     8221 tgcttttata actttaataa ttattagtcc ttttttatat gcgctatcct tgcgaagagt
     8281 tgctattgtg caagtaaatg aattattgaa agagcgaaaa tatcgtggtc cgataataat
     8341 gatggtattt ttacgagcac tacttacatt agtgttttta ggtttttttc taaacgtatt
     8401 tttctcgcca gtcattgcat ttatagcgtt aataatttgt ttggtggtat tttggttatt
     8461 cccaaaaaaa ctaacggctc gatattataa aattgaagag cgttttatta aaaatttaaa
     8521 cagtagagaa ttagccaaag caaaaagaag tagaagcgac ttaacacctt gggatggaca
     8581 catggcaacc ttcgaaattg caagagaatc aaatatttct ggaaaaactt tagaggaatt
     8641 aaaattgcga gaaaatttag ggataaatgt ggcatacatc aaacgtggag atattataat
     8701 taacatacca agtaaaaacg aacgaatttt tcccgctgat gaaattggtg ttattggaac
     8761 tgataatcag gttaaagaat taaaaatatt tttagaccaa aatgaagctg aagaaaaacc
     8821 agattataaa gaacctgata ttgttttgag acaaattaac ttaaaaaatc aaagttatat
     8881 tggtaaaagt attagagaat cgaatcttag agaaaaaact agcggattag ttgtaggaat
     8941 agaacgaaat ggaaaccgat ttttaaatcc tgaatcaact ttaatattat tggatggaga
     9001 tatattgtgg attgtaggta acaaaaaact tttaaacgaa ttgacacata actagtcttt
     9061 aaatccaatc gggtttacag actagttatg tgctaaaata gtctatctag aataatttgg
     9121 agcttcttta gtaatggtca cattatgtgg atgactttct tttattccgc tatttgtaat
     9181 tctaataaat tgaccagttt cttgtagtgt tggaatatct tttgctccac aataacccat
     9241 tccagcacga agaccaccaa taaattgttg catactttcg tttaattcac ctttgtatgg
     9301 cacacgacca acaatgcctt cgggaactaa ttttttaata tcatcttcaa catcttgaaa
     9361 atatcggtct tttgaacctt cttgcatggc ttccacagaa cccattccgc gataggattt
     9421 gaattttcgt ccttcaaaaa taattgtttc tcctggcgat tcttttgttc ctgccaagag
     9481 tgaaccaagc attacagaac ttgcaccagc agcaatcgcc tttggaatat ctccagtata
     9541 acgaacacct ccatctgcaa ttacaggtat tccagttcct tttaaagctg cagctacttc
     9601 taaaacagcc gaaaattgtg ggaatccaac gccagctaca actcttgtag tgcatattga
     9661 acctggtcct ataccaactt taacagcatc ggcaccattt tcaactaaat attttgctgc
     9721 agctgccgtg gctatatttc caactacaac atctaaatct ggaaattttg tttttacttc
     9781 ttttaatata gctacaacac ctttggtatg accgtgtgcg gtgtcaataa cgatagcatc
     9841 aattcctgcg ttatacaatg ctgttgctct atcaatagcg tcgccagtta caccaattgc
     9901 agcagctaca cgtaatcttc caaatgtatc tttattagca attggctttt gtgtaagttt
     9961 tgtaatatct ctaaaagtaa ttaaaccaac taatttatag tctttattta caacaggaag
    10021 tttttcaatt ttatgacctt gtagcacaac ttctgcttgt tgtaatgatg ttccttcggc
    10081 aacagtaacc aatttctttt tggtcattac ttcaacaatt ggtcgttcaa ggtttttttc
    10141 aaatcgaagg tctcgatttg taacaattcc ttttaaaatt tgatttttat ctacaattgg
    10201 aattcctcca ataccaaatt ctttcatagc atttttcgca tcaataactt ttgcggttaa
    10261 tggcaatgtt actggatcga taatcattcc gctttctgca cgttttactt tacgaacttt
    10321 ggcagcttgt tgttctatgg tcatgttttt gtgtaaaaca ccaattccac cttcttgtgc
    10381 cattgctata gccattgcgc tttcggtaac cgtatccata gctgcggata caaccggtac
    10441 atttaaagta atatttttgg aaaatttgga ttgaatagtt acttcgcgtg gtaaaacttc
    10501 agaataactt ggaattaaga gtacgtcgtc ataggttaaa ccttcgccaa cgattttaga
    10561 agtgtgtgcg ttcattgcaa tttgtagttg tagttaaatt gcatgcaaat ataatgaaaa
    10621 gtttaagagt ttaatggttt aagtgtttat tttgtttaaa gacttagaaa ttcttaatga
    10681 tattttgtaa aaatcttcaa cgcttcgtta tatgaacttt caaaactcat agggctacaa
    10741 ttagtagcta ctttttcggc tgtgaagtag gataggagtt tttcggtagg catgtttcct
    10801 gttaaatcgt cttttgccat tgggcaacca ccaaaacctt gaattgctcc atcaaatcgt
    10861 gtgcaacctg cattgtatgc tgcagctact ttttcatgcc atttatcagg cgtagtgtga
    10921 agatgcgctc caaattcaat atcaggatat tttggaatta aattagagta taaatagtca
    10981 ataatatctg gtgttgagct accgatggta tctgaaagag aaaggatttt aacacccatt
    11041 ttcgctaatt tttctgtcca ttgtgctaca atatctacgt tccaaatatc accatatgga
    11101 tttccaaaac ccatcgagag ataagcaact acttctttgt tggtgctctt agctatatca
    11161 agtatttcct gaagtgtaac caaactttct gcaatggttt tgtgtgtatt tcgcatttga
    11221 aaattctctg aaatagaaaa gggaaaaccc aaatactgaa tttcagaatg agcagcagcc
    11281 aattcggcac ctttagtatt ggcaattatg gctaataatt tgctttctgt ttccgacaaa
    11341 tccaattgag ccaaaacttc ggcagtgtct gccatttgtg gaatagcttt gggcgaaaca
    11401 aaacttccaa aatcaattgt atcaaatcca acacgaagta gcgattgaat ataggcaact
    11461 tttttctccg taggaataaa ttctttaata ccttgcatgg catcgcgtgg acactctata
    11521 attttgactt ttttcatcga cttcaaatat actaaagtat tctattttac gaaatcgttt
    11581 aagcaaacaa tttgaatcaa aattttatag atcaattttt gaataatttt tataaaaaat
    11641 tacacaataa tgtaataaat tagattatta cataattaag gaataattta aaatattaca
    11701 taaagaagta ataataagaa ttattacatt attaagtaat aaatttggaa attataagat
    11761 acttttttac atttgaagta tgcatagtat aataacaggc gacataattg gttctagaaa
    11821 atcaaaatca attgattgga ttgatggatt aaaagtgcta ttaagcaaat ttgggtcaac
    11881 accaaaaaat tgggaaatct atcgaggtga tgaatttcaa ctagaaatta atgatgcaag
    11941 tcaagcaata gcaattgctt tgcagattaa agctttctta aaaagtataa aacttgatgc
    12001 aagaatgagc attggtattg gtagcaaaac ctataccgca caaagtattt ccgaaagtaa
    12061 tggtagtgct tttgtctttt cgggagaagg ttttaaccga cttaaaaaga acaaaataaa
    12121 tctttctgta aattctgaat ataaagaatt taacaccgaa ataaatttaa tgttgcgttt
    12181 aggcttgact tttatggata attggacagc tcagttggca gaatatgttt tagttgccat
    12241 gcaaaatcca gatttatcac aagaagaaat tggtcaaaaa cttggcatca atcaagctgc
    12301 ggtaagtaga agaagaaaaa gagctaattt tgatgtaatg cttgagagta ttgtatttta
    12361 tattgaaaaa acaaaacaat taaaataatg gttgtatttg taaaattatt tttagcacat
    12421 attcttggag atttcattct tcagcctagt aaatgggtgg aagacaaaga acagaaaaga
    12481 ggaaaaagct tctatctata tacacacact tttttacatt ttttgcttac tataatttta
    12541 gttgccaaaa ctgattttat agtatatgga ttaattttag ccatttttca tggaggaata
    12601 gattatgcta aattgacttt tcaaaaaaca aaaaccaaac gaaattggtt tttagttgac
    12661 caagttttac acgttataag tatcgtaatt attggctcta tttatctaaa tttaaagttt
    12721 gattattcat ttttaaatac caatttttgg gtgattgtta ctgcaatact gttaattaca
    12781 aaaccaactt caattattat taaaaatatt atttcgattt ggacacccga aaacaaagat
    12841 aaaaaagaca attctctaca aaatgctgga aattttatcg gaattttaga acgcctattt
    12901 gtgttttatt ttgttttaac gaaccacatt gaagctattg gatttttatt agctgcaaaa
    12961 tcaattttta gatttggaga tttaaaagaa gcaaaagaca gaaaattgac ggaatatgtc
    13021 ttaattggaa ctttattgag ctttggaatt gcttttcttt tagcaattgt tgtaaattat
    13081 ctaactattc tttaaaatag ctttgttaat ttctttaacc aattttggac cttcataaat
    13141 aaatccagta tataattgta ccaaacttgc gccagcattc agtttatcca aagcatcttg
    13201 tgcgcaatgt attccaccaa caccaattat cggaaatgct ttattgcttt tttcagaaag
    13261 aaaacgaata acttcagtag aacgattggt aagtggcttt cctgacaaac ctccaatttc
    13321 cactttatta tttgatgtta aactctcacg agaaatagta gtattcgtag ctataacacc
    13381 agcaatttga gtttcattta caatagctat tatatcaagc aattgttcat ttgttaaatc
    13441 aggagcaatc ttaagcaata ttggttttgg aacttgtttt tcagcattca aattttgcaa
    13501 aacttgtaaa agtttcgtaa gtggttcttt ttcttgaagt tcacgaagat ttggcgtatt
    13561 tggcgaacta acatttacca caaaataatc aacataagca tacagttttt caaaacaaat
    13621 gatataatca tcaactgcat tttcatttga tgttacttta tttttaccga tgtttccacc
    13681 gattaaaata gttttatttt tttttaaatg caacacagct tcatcaacac catcattatt
    13741 aaaacccatt ctgttgataa ttgcagcatc ttcttttaag cgaaacaacc tttttttggg
    13801 gtttccatct tggggttttg gagtaactgt tcctatttca ataaatccaa atccaaagtt
    13861 ggataattct ttatatagta aagcattctt atcaaatccg gctgctaaac caactcgatt
    13921 tggaaatttc aatccaaaaa cttctgtttc caatcgttta tcttttatca catacattga
    13981 tttaaaaagt gatgaaaatc ctggtagttt ggtcaagaat cgtattaaag aaaacgtaaa
    14041 gtaatgtact ttttcaggat caaaaagaaa cagtatgggt ctaattagta atttgtacat
    14101 tttgtagtgt gttgagctgc aaaaataaga ttatatcttt agttcatcaa ctttttataa
    14161 tgatatagtt aaattttatt agatgctatt tttttgaaaa taaaatattc tatttttggc
    14221 aaaaaattaa aaatgaactt actgaatagt aatctgagta aagttacttt actaataatt
    14281 tctttaactt tcttattact cgttggtata gttgactatt atactggttc agaaatttca
    14341 tttttattat tttatttaat tcctgttttt ttcttatcac ttcatagaaa ttccaataaa
    14401 agattaataa ttatcaattc aataattggt ggtgttattt ggtttttatc tgaatattat
    14461 tcaagaaact atacaaatga ccttattcct gtttggaatg ctttagttcg attatttgtt
    14521 ttcttacttt tcggaatgtt actacgatta ctgaatgaac gttttttaaa attagaagtc
    14581 gtaaacaaac aattgcaact gttaaatatt gaaaaaaaca aattcattgg aattgcagct
    14641 catgatataa aaaatccaat tggaaccatt tcgtcgtttt ccgatttact catctctgat
    14701 ttttcagata aaatggatga tgaggcaata gaaatgattg gatatatcaa agaattaagt
    14761 tcaaattcat tacatatttt aaaaaatgtt ttagatattt caaaaattga atcaggaatt
    14821 atagaaatta aaacgaataa acaagattac atccagttca tcaaaaaaca aattttttat
    14881 tatcaaattt tagctaagaa gaaagatatt aaattaattt ttgagagtga attagagaaa
    14941 ttcatatttg aatttgatga aaattacttg ggagaagtca tctcaaattt attgacaaat
    15001 gctattaaat tttctaataa aaatgcagaa atcatcatca aggtttcctc caatagagac
    15061 aaaaaactaa aaacagaaat aattgatttt ggaaaaggaa ttccagaatc ggaacattat
    15121 aaattattca attattttca aaaaaccaca acgcaaccaa cagacggaga acaaagtaca
    15181 ggtttaggat tggctatttc taaaaaaata attaccgagc acaaaggaat tatcaatttt
    15241 actagcgaag ttaataaagg ttctaacttt tattttgagt tgtaaaaatt aatttcaaat
    15301 cgattacttt cttttaaatc attaaattat tgtagtattt tagcaattca attacaataa
    15361 aaatgatttc agaagcacaa tttcaaaaag agttagattt aataattatt aacggaattc
    15421 gggaagacat tggtcctggt gattatagtt cgttggcttg tattcctaaa gaagctaaag
    15481 gaaaagcaaa acttttggta aaagacaaag gagttattgc tggagttgaa tttgccaaaa
    15541 tgatttttaa agctgttgat gaaaatttaa aagtagaaac ctttattaat gatggagcta
    15601 aagtcaattt tggagatatt gtttttcacg tttctggaag ttcacaatcc attttaaaag
    15661 ctgaacgttt agttttaaat tcaatgcaaa gaatgtcagc tattgcaaca aaaaccaatc
    15721 attttacaca acttttagaa ggaacaaaaa ccaaagtttt agatactcga aaaactacac
    15781 ctggttttcg ggcttgcgaa aaatgggctg ttaaaattgg tggaggcgaa aatcatcgtt
    15841 ttgcattgta tgacatgatt atgttaaaag ataatcataa tgattttgct ggaggaattt
    15901 caaaagcaat tcaaaaaaca caaacttttt taacaaaaaa caaactagat ttaaaaatta
    15961 ttgttgaagc tagaacttta gatgaaataa aagaaatact tcaaaacgaa ggtgtttttc
    16021 gaattttaat tgataacttt aattttgaag ataccaaaac tgctgttgct ttaattggaa
    16081 acaaatgtca aacggaatct tctggaaaca tcaatgaaaa tactattcga agctatgcag
    16141 attgtggtgt tgattatatt tcttctggtg ctttaacaca ttcggtttac aacatggatt
    16201 taagtttaaa agcattttaa taatgagtgt tccattaaat caaaaactaa aaaaactaat
    16261 cttagttcgt caagttttat atattactaa aaaattaaaa ctgccttggc ttcaaggttt
    16321 gtcgttttat gatttaatga aaatttacat cactggaatt attgacggtg ctatttcata
    16381 tcgtgcagct gctatcgcat ggagtttttt tatggcacta tttccatttg ccttatttat
    16441 tttaaaccta attccctata taccaataga tcattttcaa gacgattttt tagcctttgt
    16501 tcaacaaagt gttccaccaa caacttacga tgccatattt actatcatta atgatatttt
    16561 gaccaaaagt aatacaggat taatttcaac tggttttttt atggctatat tactgatgtc
    16621 taatggcgta aatgctgttc ttggtggttt tgaaagttca aatcacattc aaataagttc
    16681 caaacgaaag ttttttagac aatattttgt agccttagca ctttccattt tattatcatt
    16741 agtgttgatt attacagttg ctgcaatagt tatttttgaa ttttatatcc aaaaaacaaa
    16801 attacaagac gtactttcgg atgatattcc gcttattgaa atgggaaggt atatttttgt
    16861 aattttaatg atattaataa caacttcatt actatttaaa tttggaatca aaagagaaaa
    16921 aaacagagcg tttatttcca ttggctctgt atttacaacc gtattaatca tcatttcatc
    16981 ttattttttt ggaatttggg ttgttaagtt ttcaaagtac aacgagcttt atggttccat
    17041 tggaacactt cttgtagtaa tgttctatgt ttggataaac tgcatgattt tattattagg
    17101 ttttgatttg aatgctgcca tcaataaaat taaaagagac aaacaacttg aactagaatt
    17161 attatgaaat tagtgttgtt ttttatcgtg ttttcaacct ccatttttgc tcaaactatt
    17221 gaaggaaagt ggaaaaccat tgatactaaa acccaaaaag aattatccat tattgagata
    17281 tataaaaaag acaatcaatt ctatggcaaa ttactcaaaa ttagtaacgt gttgagttca
    17341 catgctgatt gcgaaaaatg ttctgctaaa atcaaaaata attctcttat tggaaaaaat
    17401 gttattgtta aaatgcaaaa gcaagacaat caatacaaag gaaaactaat cgatcctttt
    17461 agtgataaaa gttacacttg cgtgataaaa atgctgtcta actctacact tgaaattaat
    17521 gcatatagtg ttttaccatt atttggaaga actcaaatat ggagaaaaat taattaatca
    17581 tctagtcaaa aaatgaaaaa aatagtttta gttttattgt tttattttac tcaactttca
    17641 ttttctcaat ctatagtggg taaatggacg actattgacg atgaaaccgg agtagaaaaa
    17701 tctgtagttg aaatttatga aagtggtggt gatttttatg gaaaaattat tgaaattaaa
    17761 gaagtggaac atcgaaataa aaaatgtaca aattgtaatg gcaccgacaa agacaagcca
    17821 attttaggat taattattat aaaaaaatta tcaaaagata acgatgttta tagtggtgga
    17881 actataaccg atcctaaaaa tggtaaagaa taccggtgca aaattatttt agatggaaac
    17941 gacaagctta tcgttagagg ttatattggc atttcacttt ttggtagatc acaaacttgg
    18001 aaaagaaaaa aatagtggta ttgataaatt attttattga agtagttgtt ccgctttcac
    18061 ttgataaaac tttcacctac agagtttctg aagctgaata tttgttttta aagaaaggaa
    18121 tgagagtagc agttcctttt ggtaaaaata aaatttatac ggctttagtt gttggtttac
    18181 atcaaacagc tccattactt tatgaagcca aagatattca tgaaatcatt gatgaaaaac
    18241 caattgtcaa tgagtttcaa atcaaacatt ggttttggat tgctgaatat tatatgtgtt
    18301 ctatcggcga tgtgtatcga ggtgcgatgc cgtcggcttt attgctagaa agtgaaacaa
    18361 ttattactca aaaaagcagt acctccattg atgaaagcac tttttcaaat gatgagtttt
    18421 tgatttatga agcgttgcaa catcagtcat cattaaaaat tcaagatgta atcgcaattc
    18481 taaataaaaa aacggttttg cctattattc aaaagttaat tcaaaaggaa gttattttat
    18541 tacatgaaga aattcaagaa gaatacaaac caaaacttat aaaatacatt cgattagcgg
    18601 atgaattttt aaaagaagaa aaattaaatg aactgttaaa tactatttct tcggataaac
    18661 aaaaaacact attactcact tattttcagt taaaagctca agaaagcaaa ccaatttcta
    18721 ctaaaaaatt ggtcgatacc gcttcgattt catctagttt tttaaagaaa atgattgaaa
    18781 aatcaatttt tgaagaattt cacattcaag aagacagagt taattttaga aataataaag
    18841 ccgaaaatga acttcattta agtcaacccc aacaaattgc tttcgatgaa attaaacaag
    18901 tttttttgtc aaaagatgtt tgtcttttgc atggaataac agcaagtgga aaaacagaaa
    18961 tttacatcaa actgatagaa aaacagcttg aaagtggaaa ccaagttttg tatttattgc
    19021 ctgaaattgc actaaccaca caattggttt caagactaac caagtatttt ggaaataaag
    19081 ttgctgtttt tcattctaaa tattccaata acgaaagagt agaagtttgg caaaatgtat
    19141 taaatagttc tgaaaaggca caaatagtaa taggagcaag gtctgcttta tttttgccgt
    19201 tttccaactt aggatttgtt attattgatg aagaacatga acaaacgttt aaacagcaag
    19261 atccagcacc acgataccac gctagagatg cagctatcgt tttggctaat ttttttaagg
    19321 caaaagtagt gttgggatcg gcaacaccaa gtatcgaaag ttattacaat gctacttcag
    19381 ataaattcgg tttagtttca ttaaatcaaa gatttggaaa tttccaacta cccgaaattg
    19441 agttgataga tttaaaagat agctatttta gaaaaaaaat gaaaggtcat tttagcttat
    19501 cactaattga tgccatttcg gaagctttat ccaacaatga acaaattatt ttatttcaaa
    19561 atcgcagagg attttctcca gtactagaat gcttgacatg tggtcatgtt ccgcaatgtc
    19621 cacaatgtga tgtgagtttg acctaccata aatttaaaaa tcaattgcgt tgtcattatt
    19681 gtggtcattc aattgctaaa cctacaaatt gtcatgcatg ttcaagtatc gatttggaaa
    19741 ctaaaggttt tggtacggaa caaattgagt tagaattagc ggaattgttt cctcaaaaaa
    19801 tcataaaacg aatggatcaa gataccactc gtggaaaata tagttttgaa aaaattattg
    19861 atgggtttaa aaatagagaa attgatattt tggttggtac acaaatgctt gccaaagggc
    19921 tggattttga taatgttact ttagttggca taatgaatgc tgacaacatg ctgtatcatc
    19981 ccgattttag agcttttgag agaagttttc aaatgttgac gcaagtttca ggtcgttcgg
    20041 gaagagccga aaaaaaagga aaagtactta ttcaaaccta tagcccattg cataatatca
    20101 ttcaacaagt aacgcatact aactatatag gtatgtataa agaacagtta tatgaacgtc
    20161 agatttacaa ctatcctcca ttttttagac taataaaaat tactttaaag caccgtaatt
    20221 ttgaaaaaaa taaagacggt gcaatgtggc tgtatcaagt gttgcgtcaa aatttaggat
    20281 ttcccgttct tggaccagaa gaaccagtaa tttccaggat tagaaacgaa tatattagaa
    20341 ccatcttagt aaaaattcca accgaaacct caatgcaaag cacaaaaaaa actatccaga
    20401 aaatactgaa tagttttgat atgattgcac attacaggtc tataaaagtt actgtaaatg
    20461 tagattttta ttaagcaata gctaatgctt tgattaaatc ttcttttttg tttctactca
    20521 aaggtatctt agtaacacca atttcagcaa atttactatt aaatcgctct actttatcaa
    20581 tattaataat atacgatttg tgaactctta taaacttttc acttggtaaa tctttttcaa
    20641 aagctttcat agtagataaa acaaggttac tatcttcttc ggtaaccact tttacataat
    20701 caccataagc ttcaatccac ttaattttat tagtatatac ttttaatttc tttaagttgc
    20761 ttttaataaa aatatgttct ccttcctctt cttggttttc ctttttaagc atgtggaatt
    20821 ccattgctct tctaacagaa gcattaaaac gatctagagc aatcggtttt tgtaggtaat
    20881 ccgtagcatc ataatcaaaa gccttcatag catactcagc ttttgaggta atgaaaatga
    20941 tttgtggttt ggtttttaaa ccatcaagaa aatcaaatcc acttattact ggcatttcaa
    21001 tatccaagaa tattaaatcc acagtatgaa cagacatgca atttttagct tcaattgcat
    21061 tagaaaaatc tccaactaaa tgtaaatttg gatgattatt tactaacttt gcaatgatca
    21121 ttctctgtat agagctatca tcaactacga cacaatttaa tttcataatt tactttatta
    21181 tagatttggt tcggtaaaag tatatagaaa aaatcaaaca acctaacatt tcgtcgattt
    21241 tttttgcctt taatcgattt attttacttt tcgacgattt attaacattt gtagtaaaat
    21301 aataaaatgt tattcttact tttgcgccca attataaatt ttaatcatat tattatgaat
    21361 cattacgaaa ctgttttcat cttaaatccc gttttatctg aagttcaggt aaaggaaaca
    21421 gtaagcaaat ttgaagattt tcttactgca aaaggagcaa agatggtatc aaaagaggat
    21481 tggggcttaa aaaaactagc ctatgaaatc caacacaaga aaagtggttt ttaccattta
    21541 tttgaatttc aacttgcagg cgagcattta attgcttttg aaactgaatt tagacgtgac
    21601 gaaagagtaa tgcgtttctt gaccgtaagt cttgataaac atgcagtatc ttgggcagaa
    21661 agaagaagag aaaaattaaa aactaaagcg taattactat gtcaactatt gagcaaaatg
    21721 caaaaggaaa aaaagacgga gatattagat atttaacgcc tttaaacatt gaaacttcaa
    21781 aaactaaaaa atattgtcgt ttcaaaaaat ctggaatcaa atacatcgat tataaagatg
    21841 ctgacttctt attaaagttt gtaaacgaac aaggtaaaat tttgccacgt cgtttaactg
    21901 gaacttcttt aaaataccaa agaaaagttt ctgttgcagt aaaaagagcg cgtcacttgg
    21961 ctttaatgcc ttatgtagcc gatttattaa aataatatta aacaattaaa gttgttggtt
    22021 ttcaataata tgaaacctaa cttcttctaa aaaaaggaca acaacatgga acttatttta
    22081 aaacaagacg ttcaaaattt aggatttaaa gatgatgtag taacggtaaa aaacggttac
    22141 ggtcgtaatt atttaattcc acaagggttt gctacactag caactccttc tgctaaaaaa
    22201 gtactagccg aaaatttaaa acaaagagct tataaagaag ctaagattgt tgctgatgca
    22261 acaactttag cagaatcaat caaagcttta gaaattaaac ttatggcaaa agcgggtggt
    22321 gaaaaattat tcggttcaat ttcaaacatt gatattgcta atgcattaga agctgctgga
    22381 cattcagtag atagaaaatt cattactagt ggaattgtta aaagaactgg aaaatatact
    22441 gcttcagtac gtttacacag agaagtaatt gttgaattac catacgaaat tattgctgag
    22501 caagcataat taatttctac aaatacatta aatcccaatt ttatgaataa taattttggg
    22561 attttttatt gctaaaaact aaacattttt taatttgaaa tacaaaagac ttactaaaga
    22621 acaatttgaa gagcttcatg ttgagttctc aaacttttta gccacccaaa gcattgataa
    22681 aaaagaatgg gacactatta aagctaataa gcctgagatt gccgaacaag aacttgacgt
    22741 tttctctgat ttaatttggg aaggtgtttt aaaaaacact cattatttag aacatttttc
    22801 caaaaatcat atatttttat ttcactgtca agaaaaatta attcaatcaa ttgtgttaaa
    22861 atcattagac acaactattg attttatgaa taaagaagga ctgcaatggt tgagtgacaa
    22921 catgtttact gagcaggttg aaatacattt aggaaaaaaa gaatatgaaa cactacgaaa
    22981 cgaatcaatt tttaaattaa ttaccgaagg tgctatttta tccgatgggc aattgtatct
    23041 tcaaataaac gggataattc aatcgtaaag aatgattatt ttaaaattaa ttggttattt
    23101 tagtactcaa atttgatact aaaatagcca attttttatg gatattcagc aaactatttc
    23161 caatcttaga gatgatctca atcagcataa ttacaattat tatgttttag atacaccaac
    23221 aatttccgat tttgaatttg atcaaaagct aaaacgactt caagaattag aagctcaaaa
    23281 cccagagttt tttgatgaaa attctccaac acaacgtgtt ggtggaacaa ttactaaaaa
    23341 ttttgaaacc atcgtgcacg attatagaat gtattcattg gataattctt attctaaaga
    23401 agacctgata gattgggaaa ctagaattca aaaagtactc ggaaatgttg ctttggaata
    23461 tacttgtgaa ttgaaatacg atggtgcgtc catttctatt acctatgaaa atggaaaatt
    23521 aattcgagcc gttactcgcg gtgatggttt tcaaggggat gatgttacga ataatattaa
    23581 gaccataaaa tcaattccac ttcaattgaa agggaatttt ccaaaaagat ttgatattcg
    23641 tggcgaaatt gtactgcctt ttgaaggttt tgcaaaaatg aaccaagaat taatagaaat
    23701 tggagaaact ccctactcaa atccaagaaa tacagcttcg ggaagtttga aacttcaaga
    23761 cagtagtgaa gttgccaaac gaccactcga atgtctgctt tatttcattg tgggaaatca
    23821 tcttggttta aaaacacagt ttgaaagttt agaagccgct agaaactggg gtttcaaagt
    23881 gccaaaagaa gcaaaattag catcaaattt agaagaagtt tttgtattta ttgaatattg
    23941 ggataagcac cgacatgatt taccttatga aactgatgga gttgtgatta aagtaaatag
    24001 ttttcaacat caagacgaat tgggctacac agcaaaagca ccacgatggg caatggcata
    24061 taaatttaaa gctgaaagag tttccactac tttaaattca attgtgtatc aagtgggaag
    24121 aacaggcgcg attacgccag tggctaattt agaaccagtt caattagcag gaacaattgt
    24181 aaaaagagct tcattgcata atgctgacca aattgcaaaa ttagatattc gtgttgatga
    24241 tactgttttt gttgaaaaag ggggtgaaat cattccaaaa attattggcg ttgattttga
    24301 aaaaagggct tcaatctcga aaccaacaac ttatatcaca aattgtcctg agtgtaacac
    24361 caaattgaat agagtagaag gtgaagcgaa tcattattgt ccaaattttt atggttgtcc
    24421 accacaaatt ataggaagaa ttgaacatta tatttctaga aaagcaatgg atattgacgg
    24481 acttggtggc gaaacggtag ctttgctttt caataatggt ttagtaaaaa attatgctga
    24541 tttatatgaa ttgaccgtgg aacaaatttt accattagat agaatggcgc aaaaatcggc
    24601 agaaaattta gtcaatggcg ttcagaaatc taaagaaatt ccatttgaaa gagttttata
    24661 tgccatcggt attcgctatg ttggtgaaac tgtagccaag aaattagcca aacattacaa
    24721 aactattgag gcattgcaac aagcaacttt aatggatttg gttttggttg atgaaattgg
    24781 agaaaaaatt gcacaaagtg tgataacctt ttttgaaaac gatgaaaatg taactaccat
    24841 aaatcgatta aaaaagtatg gtgtgcaatt ggaaattatt gaaaaaacaa acccaaatgc
    24901 aacttcaata ttaaatggaa aaacattcgt tgtttctggt gtttttagtt tgttttctag
    24961 agatgaatta aaacaatcta ttgaagataa tggaggcaag gtaggcagct ctatttctac
    25021 gaaaacaaat tttgtaatag caggtgaaaa tatgggtcca gcaaaattag acaaagcaaa
    25081 tcaattgaaa attcctatta tttctgaaaa tgaatatgtt aacttgttga aaaatggaat
    25141 tgaataaaaa aaaacactat tttaacaaca ttaatttgat taagatgtct tataaagaag
    25201 ctaaaattcc attacatcta tttgttgttt caagtttagt ctatataatt tctaacttta
    25261 tggaaataga aatagttggt ttattagtta aaccaataat tattccatct atttttttct
    25321 attatttaat gactaaaaag aataatatta atatttcttt ttcattaatt tttatattgt
    25381 tttttattgg agatatgatt attatgctct attcgcaaga atttatgagt ttaatattag
    25441 ctccatttat gttttcatac atcattctct tgaaatttat ttttgaagat ataaatttta
    25501 aagcaaaaag tatttatact attttttatg cagtattagt gtttgtacta ctgagtttaa
    25561 ttctttatta cattttggat ttacctgtag ataaggttat taataataac atactttttc
    25621 ttttttatgg aattttattg ataactttag taacaatttc aacctatact tatttaagta
    25681 atcaaaactt taacacactt aatttgttgt tgatggcaat agcaatgctg acttcagatt
    25741 tgttgtattg tatcaataag ttcattttta gtttttatat tattgatagt ttgaatttgc
    25801 tagcacagtt tgcttcctat tattttatgg ttgtttattt taattcacga ataaatacaa
    25861 acacaatttt gcttttaaaa agagaataaa tgaaacgttt tcttgggaaa aaaatatttc
    25921 gtctttttat cattgcttac tttacagtag ctatgataga aattgttgcg gaatatttta
    25981 atgataaaac tctagttacc ctattcaaac caattttgat tccattattg ataggttact
    26041 atttttataa ttcaagaaaa agaaattttt tatttattgt agctcttgtt ttttcttgga
    26101 tagctaatat tttttttatt aacgacacca taaattatat cattttagga tctgtttttt
    26161 tcttaatata tagagtgata attatttatt tagtgattcg aatggtaaaa atgccaagta
    26221 aattgccatt attaattggg gcaattccgt ttcttttcat ttatgcaact acgtgctttt
    26281 taacctttga ggattttggt gaaaacattt ttttgtttat cattcatgga atatttatca
    26341 tctttttagg tggttatagt ttaggaagtt atattgtata ttcctccaaa actaatttaa
    26401 tcctcttttt aagtacaatg ctattcgctt tatctcagtt tgtatttgtg ctaaaaattt
    26461 attcagaata tgtttatttt ttacacgcat tagcgatgtt gcaatttgta gtggcacaat
    26521 acttattgac caagtttatg catctaaagg aaaaaccaaa atcaaaatat gagtttgcaa
    26581 atactataaa cgaattgtga aaaacattat ggtaagtaaa ttaaacatcc taaaaggttt
    26641 tacaatattg tattttatta tttcaattat tgaaataaca gcagaatatt tgtctaataa
    26701 ggctatgatt atagcactaa aacctttaat accattagta ataattattt tatattttat
    26761 tgaaagcaat aaaaaaaata agctctatgt agttgcaatg tttttttcaa tacttactaa
    26821 cctattgttt attccaagta ttgctgtttt tcttttttat ggaataatag cttttttaat
    26881 acatcgaatt attattatct atttgttagt aaaaataaac cctatcaaag atttcattcc
    26941 ttttttatta gcaacagctc cttttctgtt ggttttcttt tatttattta tagaaacaga
    27001 aaacatcccc gaaaacagtt atgccatttt agtattacaa aacatattaa tgtcaatatt
    27061 tgcaggaatt gcgctgtcta attatgtaat gaatgacaac aaacaaaatt caattctatt
    27121 aataagtgca ttcctttttg tattacttca atttgtgatt tttatagaaa aatatttttt
    27181 aatctatgaa ttaaaagaaa tttttagacc gatggcaatg ctgttgaatg ttttcgcttt
    27241 ttacactcta tttagatata taattgaaac agaaaaatta aacaacgatt gatttggctt
    27301 tacttg
//
LOCUS       DAOOBH010000021        27165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_2333_length_27165_cov_5.686057, whole genome shotgun sequence.
ACCESSION   DAOOBH010000021 DAOOBH010000000
VERSION     DAOOBH010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 27165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27165
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_27165_cov_5.686057"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3099
                     /locus_tag="PK218_04045"
     CDS             <1..3099
                     /locus_tag="PK218_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HPW97713.1"
                     /translation="EISFNRLDITSANCIPNTVLGVNTLSPFDDFQWYFNGVLTTETS
                     GSINPIVPGYYKVSATIAGCGTPKESDNIPVSLCPLDTDNDGVNNNIDLDLDNDGLTN
                     CEESYGNHNLNLANNPVGFLQVSNYINTFLTTLTASSSGTPSATPFVGNVDGNFTTET
                     NSGKDSSISYEIAFNNPISLSLEYNTLATSADVLTLATEVRVSCPANRTLTVLNPDDQ
                     ILIDTNFDGIYESGVTQFSSFEIRFRLNGSSALAPGTGTFSIRGNTITTLKVTNINIA
                     EFGKSKVGLHITATCVEKDSDNDGIPDQYDYDSDNDTIPDRIESQGQNILALSGIDAN
                     NDGIDDVFGNGILPANTDNDTVPNYLDSDSDNDGIFDIIESGSTGNSNNTSGITVGNV
                     GANGLDNTLETSPDSGTINYTLTDTDGDGIYNYMELDSDDDGCNDVIEAGFSDPNNDG
                     ILGDDNPTVFDTQFGLVISSSGYGVPNANYTIAAPISISAQPQSLQVCELQTATFTVT
                     SSPVDSYQWQISTDGGITWTNVINNATNAGATTNALTVSTVVPATPNHQYRAFLNRIG
                     NSCGLYCNAVELTTYALPVVNTPIDLKQCDNDTDGISVFNLTQKNDFISANYLNETFS
                     YYTNQTAANTQNNSFLITNPIAYTSGIATVYARVENSNGCYSIATINLIVSVTQIPAN
                     FAIPIMHECDDYIDAINDDFDGISAFNFTTIQNSLLAILPTNVSVSFYKNEADFLAET
                     DSDGNSLAITDISNYRNTDYPFQQTLWVRADSILDNSCYGFKTFEIRVVPWPRLDLLE
                     TVLICLPETQTTINAGIIDGSPTSNYTYQWYSNGSPLPGETNYSLTVSSPATYSVDVK
                     TIYNCIRTRVIEVVGSQIASLLSIEISDLTQINTVLINVTGSGIYEYSLDDIAGPYRS
                     SNFFTNVPMGLHDVYIRDTNGCGILGPLPIAVLGIPQYFTPNGDGYNDTWNVKGINQT
                     NVQSIIYIFDRFGKLIKQISAVSPGWDGTYNGHQLPADDYWYNIQFQDGRNARGHFSL
                     KR"
     gene            3167..5170
                     /locus_tag="PK218_04050"
     CDS             3167..5170
                     /locus_tag="PK218_04050"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HPW97714.1"
                     /translation="MLFKRYYILLLLFFGTASAQYISTDDTRTPLQLIEDVLINSGCA
                     SVSNVSVSGGNFATGDTSYGYFNANGSSFTFPEGIILSTGKIIDARGPNYFISDDTAP
                     GWTGDQDLNYALGISNSINATVLEFDFIPIGNQISFDYIFASEEYHGSATCTYSDGFA
                     FLLKEVGTTNYQNLALIPNTAIPVKVTTVHPEILGGCPAQNEQYFDAFNGPEYPTNFN
                     GQTKVLTARATVIPNTPYHIKLVIADEGNYRYDSAIFLRGGSFNLGLDLGDDRTLANR
                     NPVCFDENLTLNATSVGAQSYQWNFNGSPIVGQTASTLTFNPPYSTTQNGIYAVAVTY
                     SLTCTIVSDIELEFSPELQANQTSYNFCDTEGAQDGISTITLSDITPTLFTNLPANFQ
                     VNYFSSITSTTPLSNLFTLTTPFQQIIYVRIANNNCYNNIPVTLNIQVFTDTILDETL
                     GICNNDPTILTADSGYPSYLWNTGETSPSIQVTMPDTYTVTIGNNDGCTKVKTFTVVG
                     SEIATITDIIINDFNDNNSIEIIAIGNGDYEYSIDGVHYQSSTIFYNLSKLEYTAYVK
                     DKNRCGIATHVFYLLQIPKYFTPNGDGFNETWNIRNLSERGFNQTIISVFDRYGKLLK
                     QFSGNSTGWDGTYNGKNLLADDYWFSISNQGKELFRGHFTLKR"
     gene            complement(5575..7371)
                     /gene="typA"
                     /locus_tag="PK218_04055"
     CDS             complement(5575..7371)
                     /gene="typA"
                     /locus_tag="PK218_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPW97715.1"
                     /translation="MESIRNIAIIAHVDHGKTTLVDKIMYHCQLFRENENTGDLILDN
                     NDLERERGITITSKNVSVVYKGTKINIIDTPGHADFGGEVERVLNMADGVCLLVDAFE
                     GPMPQTRFVLQKAIDLGLKPCVVINKVDKENCTPEEVHEKVFDLMFELGAEEWQLDFP
                     TVYGSAKNNWMSDDWKNQTENIEPLLDMVVANVPAPKVSEGTPQMLITSLDFSSFTGR
                     IAIGRLERGVLREGMPISLVKRDGKVIKSRIKELHTFEGLGRKKVQEVIAGDICAIVG
                     VEGFEIGDTIADFENPEALQTIAIDEPTMSMLFTINDSPFFGKEGKFVTSRHIRDRLT
                     KELEKNLAMKVGETDSADKFMVFGRGVLHLSVLIETMRREGYELQIGQPQVIIKEVDG
                     VKCEPIEELTIDLPENLSGRAVEFVSVRKGEMLSMEGKGERMVVKFNIPSRGIIGLRN
                     QLLTATAGEAIMAHRFIGYEPYKGEIPGRNNGSLISMENGKAIPYSIDKLQDRGTFFV
                     DPNEEIYAGQVIGENTRSDDMAVNVTKTKKLSNVRSSGADDKARIIPAKRFSLEEALE
                     YIQKDEYVEVTPKNIRLRKIFLDENDRKRNKI"
     gene            7514..9226
                     /locus_tag="PK218_04060"
     CDS             7514..9226
                     /locus_tag="PK218_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPW97716.1"
                     /translation="MKSNSNKTTFIFLLLLSFSALGLSFSLSYFNNGTINTDLLFVEN
                     IILIVIAGAIFKYITHRNKLRNKAEFENLINVNKDIIEAKERYDIVAKATSDTIWDWR
                     IEEDIMIWNKGIQGVFGYDKKEIEKSSKWWFERIHPEDSLKMSVRLYSFLDFVKDRWQ
                     DEYRFRCADGTYKHVIDRGFLIKDKSGKIVRMIGAIQDVSKEKEEEERLRLLETVITN
                     TKIAIAIANIELNETQYPKIWYVNPAFSEITGFSREEVVSKDFSILFNKNTDVLQLEN
                     LKNAIKEYRECTFETACSKKDGTPFWISLSMIPVNDFEGRHSHWISIQRDITIEKEKI
                     KEREQLIKELTKNNNDLKQFSYITSHNLRAPLSNLTGLLNLIEDIPIENEDLKEIIDG
                     FANSTYMLNETINDLAKVIIIKDSSAIKKEKILVKDVFENVFNQVSFLINTIKPILKI
                     DLEETYVLNSNKSYLESIFLNLMTNSLKYKSTKRQLKINVTSKVEGDEVIIVFEDNGI
                     GIDMERNKNKIFGLYQRFHNLPDSKGLGLYLVKSQVEAMDGTISVESEVEKGTTFIIK
                     FKNQ"
     gene            9227..9616
                     /locus_tag="PK218_04065"
     CDS             9227..9616
                     /locus_tag="PK218_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW97717.1"
                     /translation="MLHKVLCVDDDPITLMLCKKVIERVNYTKEVDTAKNGEDAIAYF
                     NQLKNNQEIPPELVLLDLNMPIMNGWELLDAYLELEFNLIFKETKFIVLSSTIDPSDI
                     EKSKSYPMVIDFISKPITKEILAEIKL"
     gene            complement(9668..9919)
                     /gene="rpsT"
                     /locus_tag="PK218_04070"
     CDS             complement(9668..9919)
                     /gene="rpsT"
                     /locus_tag="PK218_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPW97718.1"
                     /translation="MANHKSALKRIRSNEKRRVLNKYQHKTTRNAIKALRIATDKSDA
                     SAKLNAVISMIDKLAKKNVIHDNKAANLKSKLTKHVAKL"
     gene            complement(9949..10020)
                     /locus_tag="PK218_04075"
     tRNA            complement(9949..10020)
                     /locus_tag="PK218_04075"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9985..9987),aa:Glu,seq:ttc)
     gene            complement(10099..11577)
                     /gene="proS"
                     /locus_tag="PK218_04080"
     CDS             complement(10099..11577)
                     /gene="proS"
                     /locus_tag="PK218_04080"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPW97719.1"
                     /translation="MSKNLTKREEDYSKWYNELVVKADLAENSGVRGCMVIKPYGYAI
                     WEKMQAELDRMFKETGHSNAYFPLFVPKSMFEAEEKNAEGFAKECAIVTHYRLKNDPD
                     NKGKLMVDPNAKLEEELIVRPTSEAIIWSTYKTWVQSYRDLPLLINQWANVVRWEMRT
                     RLFLRTAEFLWQEGHTAHATKQEAIDETIQMMNVYADFAENFMAIPVVKGIKTENERF
                     AGADETYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFANQEGKQEHVWGTSWGVST
                     RLMGALVMTHSDDNGLVLPPNLAPIQVVIVPIFKTDEEFHQITEVANELVSQFKKANV
                     SVKFDNRTTHKPGFKFAEWELKGVPVRIAIGPKDLENGTFEIARRDTLSKEVKEKEGI
                     VAYISELLETIQKELFEKALEYRNSHITEVNSFEEFKEVLENKAGFVAAHWDGTGETE
                     EKIKELTKATIRCIPLNRKLEKGICVFSGKESVGRVLFAKAY"
     gene            11679..12674
                     /locus_tag="PK218_04085"
     CDS             11679..12674
                     /locus_tag="PK218_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97720.1"
                     /translation="MKTILQFTQKNSYYLIIGSLLFLISSCGSYQNTSYYDNDGVYSS
                     RTNTTTTENNSKAKSNQYQEYFSSLNQDNNEFFVDVDNYSSVQNDTVTQNNNNATESY
                     SGWGNNPQTVVVNVYDNNWGYGYWNNYWYGNYWVNNGWNWGIGWNSWYGPSIGWGWNN
                     WYGNYWGWNGWYGNYWGYNYGYSPYWGYNHGHYANWNGGTRGNNYNYYNGSRGRNSYT
                     TGRNSITNGTRNNSFGTSRNNTRGNSYSTTRNTTYSNSANVRTNNYNNTRGNSTSTNS
                     YNSTTRSNNSTRTNTSSSPVRSNTQSSTNYGGTRSSSGSSTSGSSGGGRSSGGRR"
     gene            12706..14214
                     /locus_tag="PK218_04090"
     CDS             12706..14214
                     /locus_tag="PK218_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HPW97721.1"
                     /translation="MKIIYTFMFFGLTVFTIKAQETTINDALRLAVDNITGTARYRSM
                     SGAFGAVGGDLSSINQNPAGSIFFNNNLATGSISYFGVRNKSNYFGTQTTENDNSLDL
                     NQAGVVFVFNETSKSTDWNKISLAFNYENTNNFNNTIFSAGTNPYNSIGNYFLDFAQG
                     IPLDYLKNINFNQLYFNEQQAYLGYDTYIFEPFTNASDNVDYYTNIPTGGNYYQENYT
                     NTNGYNGKLTANFATSYKNKLFLGANLNVHFVDIQKVFNIYESNNNPVYDEGSTIGSI
                     FFRNELFTFGSGISFNLGAIYQPIDNIRVGVAYESPTWYRLTDQLTQGVVTTSINNQD
                     NNSRPSSFQDTQEFLPYYIQTPSKWTGSLAYIFGKRGLISADVSMKDYSVTRFKPKNS
                     QPYTSLNTTMDSILDNAMEVRLGGEYKIKQFAIRGGYRFEQSPYKIDQAFGDLTGYSG
                     GIGYTFGENRIDLAYTHEYRNMNQAFLSSGMTDMARINRKNNTVTVSYTINF"
     gene            14320..15627
                     /gene="rimO"
                     /locus_tag="PK218_04095"
     CDS             14320..15627
                     /gene="rimO"
                     /locus_tag="PK218_04095"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPW97722.1"
                     /translation="MRTKSLKKNKINVITLGCSKNTYDSEVLMGQLKASGKDVVHEQE
                     GNIVVINTCGFIDNAKAESVNTILEYADKKERGIVDKVFVTGCLSERYRPDLEKEIPN
                     VDQYFGTTELPLLLKALGADYKHELLGERITTTPKNYAYLKIAEGCDRPCSFCAIPLM
                     RGKHVSQPIEKLVKEAEGLAKNGVKELILIAQDLTYYGLDLYKKRNLAELLENLVKVE
                     GIEWIRLHYAFPTGFPMDVLELMKREPKICNYIDIPLQHISDNILKSMRRGTTYAKTT
                     QLLKDFRAAVPEMAIRTTLIVGYPGETEEDFEILKNWVQEMKFERLGCFAYSHEENTH
                     AYLLEDDVPEEIKQARAMEIMDIQSQISWDLNQEKIGQTYKCIIDRKEGQYFVGRTEF
                     DSPDVDNEVLIDASKFYLKTGDFTNIKITDATEFDLYGEPENN"
     gene            15632..16054
                     /locus_tag="PK218_04100"
     CDS             15632..16054
                     /locus_tag="PK218_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97723.1"
                     /translation="MKKILLILFLSLSLFSFSQMDRRIGRIPQSTSTKKKEKIDPIQL
                     SLEYLKKELNLDSFQEAATKTYLEENQKEKEYILTLDIIDNDKVEKLKVSYEKMDAQI
                     DALLNKKQREAFIKLKEKRNGKDKKNKKDNKEEELENQ"
     gene            complement(16123..17664)
                     /locus_tag="PK218_04105"
     CDS             complement(16123..17664)
                     /locus_tag="PK218_04105"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPW97724.1"
                     /translation="MQDKIKLVHDKIALAKIGGGEKRIAAHHAKKKLTARERVEYLMD
                     EGSFEEIGMLVTHRTTDFGMEKEIYYGDGVVTGYGTINGRLVYIFAQDFTVFGGSLSE
                     THAEKICKVMDMALKNGAPMIGLNDSGGARIQEGVRSLGGYADIFYRNVQSSGVIPQI
                     SAIMGPCAGGAVYSPAMTDFTMMVEGNSYMFVTGPNVVKTVTNEEVTSEELGGASTHS
                     TKSGVAHITSPNGVECLEDIKRLLSYLPQNNREIPPKLPYVFEDELREKLDTIMPDNA
                     NKPYDMHDVIGNIIDEDSFYEIHKDFAENIIVGFARLGGRSIGIVANQPMVLAGCLDV
                     NSSTKAARFVRFCDAFNIPLLVLEDVPGFLPGTDQEWNGIIVHGAKLLYAFSEATVPR
                     ITVITRKAYGGAYDVMNSKHIGADMNFAWPSAEIAVMGAKGASEIIFKKEINEAENPA
                     QKLAEKEAEYAELFANPYTAAQRGFIDEVILPSQTRRKLIKAFSMLENKVVDTPKRKH
                     GNIPL"
     gene            complement(17716..19164)
                     /locus_tag="PK218_04110"
     CDS             complement(17716..19164)
                     /locus_tag="PK218_04110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPW97725.1"
                     /translation="MTKILVANRGEIAIRVMKTAKKMGIKTVAVYSTADRNALHVKYA
                     DEAVWIGEAASSQSYLRGDKIIEVCKNLAVDAVHPGYGFLSENAQFAELCEKNNIIFI
                     GPKSKAIEMMGNKLAAKEAVKDYDIPMVPGTEDAISDIEEAKEIATQIGFPILIKAAA
                     GGGGKGMRIVEKEADFTSQMNRAISEALNAFGDGAVFIEKYVTKPRHIEIQIMADSHG
                     NVLYVFERECSIQRRHQKVVEEAPSCILTPKKRKEMGEAAVKVARACDYLGAGTVEFL
                     MDADLNFYFLEMNTRLQVEHPVTEMISGLDLVELQIRVARGEALDMKQEDLKIKGHAM
                     ELRVYAEDALNDFLPNVGFLNTYKLPEGEGIRVDNGIAEGMDVPIYYDPMLSKLVTYG
                     KTREEAIALMIKAIENYHVEGVATTLPFGKFVMEHEAFRSGDFDTGFVKAYYNPEKMK
                     AKLDTEAEIAAMIALQQYLDDKKIVRLPNQEQ"
     gene            complement(19277..19762)
                     /locus_tag="PK218_04115"
     CDS             complement(19277..19762)
                     /locus_tag="PK218_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPW97726.1"
                     /translation="MPSKYKLSVNNSIHFILDEIELKHLDAVRDGKTKFHVLKENQPF
                     QVEIITTNFLAKTYTIKVNNNTYEVAISNDLDQLISSLGIERGKTKVVNAIKAPMPGL
                     ILEVSVSVGQTVKENEPLLILEAMKMENSFLSPRDGIIKSIAVEKGNAVEKGQLLIEF
                     E"
     gene            complement(19851..22070)
                     /locus_tag="PK218_04120"
     CDS             complement(19851..22070)
                     /locus_tag="PK218_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW97727.1"
                     /translation="MKDILLITPPFTQLNTPYPATAYLKGFLNTKQIDSFQMDLGIEV
                     ILQLFSKEGLNNVFCHIEGSIDANTTTSNSQRIYSLREEYIKTIDNVITFLQGKNPSL
                     ARQICSRNFLPESSRFNQLDDMEWAFGIMGMQDKAKHFATLYLEDLSDFIVEIVDENF
                     GFSRYAERLGRSANSFDELYSKLQEDFTYIDGITLKILKERIEEVRPKLVLLSVPFPG
                     NLYSAFRCAQWIKKYHPTIKIAMGGGFPNTELRSLKDKRVFEFFDFITLDDGELPVEL
                     LNQNVCLTESSQSASKSEFKRTFLLENNEVIYKNNSTKNDYKQSEIGTPDYSDLLLDQ
                     YISVIEIANPMHSLWSDGRWNKLTMAHGCYWGKCTFCDISLDYIKLYEPIAAKTLVNR
                     MEELIAQTGENGFHFVDEAAPPALMREVALEIIRRKLIVTWWTNIRFEKSFTHDLCVL
                     LKESGCIAVSGGLEVASDRLLELIKKGVTVEQVAHVTRNLTESGIMVHAYLMYGYPTQ
                     TIQETIDSLEMVRQLFEVGVLQSGFWHQFAMTAHSPIGLNPEEFGVIPKNTSFGEVLF
                     ANNDIEFKDKTGINHEKFSFGLKKSLFNYMHGICFEYELQDWFDFKIPRTKIAPDFII
                     SCLEKEANFSVKPTAKVFWIGGKPILEHYTKSKKGTTWQMTKMTFYQKIKTIEINLES
                     EKAFWLKKNLEKISIENMFSMTFSQLKSDFETTMQDFELFWYSKPIVTLRDSGLLVL"
     gene            complement(22120..22836)
                     /locus_tag="PK218_04125"
     CDS             complement(22120..22836)
                     /locus_tag="PK218_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HPW97728.1"
                     /translation="MRKIVLSLIIVIFIISCKEEAPSTKNFVLTGNIKGLKEGKLYIQ
                     RIKDTILIPIDSITISGDSNFKSEFDLDSPEMLYLFLDRGVTNSLDNNIPFFAEPGKM
                     NIETSLDFFTADVKITGSKNQELYDEYTKVISRYVDKDLDLITKRFEALKNKNSKEIE
                     EIQKKQDAIKRSKYLYTANFAVNHPDAEVAPYVTLAEIYDINFKFLDTIQKTMTPRVA
                     KTLYGKKFMEYYLERKKMGK"
     gene            complement(22947..24803)
                     /locus_tag="PK218_04130"
     CDS             complement(22947..24803)
                     /locus_tag="PK218_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HPW97729.1"
                     /translation="MKKHIVTALLIVLNWNVIVFAQNKQNATPVKAIIDLNTIEDDRV
                     KVVMIPQKTTKEEIIFQIPKTVPGTYSADNYGRYIDELKAFDTNKKELKVEKTDDNTW
                     KISNAKTLSQIVYFVNDTYDTETGRGFGKEEIFSPAGSNILKGKNFMLNNHCFIGYFD
                     GNPDVKYSVTINHPSDLVGSTSLNDADKSPTIDVFNVERYFDLTDNPIMYCKSNSETF
                     TFDGMEILFSVYSPNGVHTAKSLLPDLEKMMRAQKTFLGPINDNKKYTVLLYLSESNK
                     RDAKGYGALEHHTCTSVVFPEEMPADMLGKQIIDVVSHEFFHIVTPLSIHSKEIQYFD
                     YNNPKMSEHLWMYEGVTEYFANLFQINQGLIDQDEFYQRMLGKISNASRFDDTMSFTE
                     MSKNVLVKPYKDQYLNVYEKGALIGMCIDIIIREKSNGERGILDLMKKLSKEYGVNKP
                     FNDDELFAKITSLTYPEVGQFLITHVSGTTPIDYELYFTKMGVGKGKNSVPANPFLKE
                     QTPYIKVNPATKEIAVATNLELNDFFKNLDIKPDDIILAINNVNYNLDNIYEMVMESQ
                     NWNQDDAISVKIKRDGKEQTINGKVKLTMEEIDAYKLIDESKAKLNQAWLKG"
     gene            complement(24889..25170)
                     /locus_tag="PK218_04135"
     CDS             complement(24889..25170)
                     /locus_tag="PK218_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HPW97730.1"
                     /translation="MHKKVFTEVEIDRIIEMAWEDRTTFDAITFQFGISEQQTIEIMR
                     RELKLSSFKLWRKRVQGRVSKHAKLRVNTFGRFKSRLQRQITQNKISKR"
     gene            complement(25224..25499)
                     /locus_tag="PK218_04140"
     CDS             complement(25224..25499)
                     /locus_tag="PK218_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HPW97731.1"
                     /translation="MKKSRQVTLDNEQLERVVTMAQEEKKPFETIKAEFGINENEVIE
                     ILKKRLTKDKFELWKKKAQGNKPKIKPKFDDFDEDLEGKYYIKNKFD"
     gene            complement(25593..26417)
                     /locus_tag="PK218_04145"
     CDS             complement(25593..26417)
                     /locus_tag="PK218_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HPW97732.1"
                     /translation="MNVIISYFSSIPSSHRAIILAGGITFFWLIESAIPFIKFNYNKW
                     QHARINIFFTLTTVIVNFALAVFIFKTANWTTETQFGILNWLPTMNPWFFAIVGLLLL
                     DLIGAYLVHLIEHKVKFLWRFHLIHHTDTWIDITTANRHHPGESVLRFVFTIVGVFIV
                     GAPMWMVFLYQTLSVVATQFNHANIVLPKRLDVFLSYFIVSPDMHKVHHHYVLPYTDC
                     NYGNIFSIWDRLFGTYMTLDREKLIYGVDTHMKPEEHNRLTNLLRIPFQKNKNTIH"
     gene            complement(26429..27031)
                     /locus_tag="PK218_04150"
     CDS             complement(26429..27031)
                     /locus_tag="PK218_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HPW97733.1"
                     /translation="MNSLFPKDKLIFDIPNAEVEYYGQFFDEIQANKLFETLKNEIPW
                     QQDMITVFGKTHPQPRLTSLFGNDGKQYSYSNIIMQPNPWIAVLTFIKEEVEKICHEK
                     FTTVLLNYYRDGKDSNGWHADNEKELGINPILASVSLGAERMFHLKHNLINDQMLKIN
                     LEHGSLLVMKGETQHFWKHQIPKTAKSIGSRINLTFRILK"
BASE COUNT         8919 a         4813 c         4186 g         9247 t
ORIGIN      
        1 gaaatttctt ttaatcgatt ggacattact tcggctaatt gcattccaaa tactgtttta
       61 ggagtaaata ccttaagccc attcgatgat tttcaatggt attttaatgg tgttttaact
      121 accgaaacca gcggttctat taatccaata gtacctggtt attacaaagt ttcggcaact
      181 attgcaggtt gtggtacacc aaaagaatca gacaatattc cggtaagtct ttgtccttta
      241 gacacagata acgatggcgt aaacaacaac atcgatttag atttagacaa cgatggatta
      301 accaattgtg aggaatccta tggcaatcat aatttgaatt tagcaaataa tccagttggg
      361 tttcttcaag taagcaatta tatcaatact tttttaacaa cgttaactgc ctcttcatca
      421 ggaactcctt cggctactcc ttttgtcgga aatgtcgatg gaaattttac tacagaaacg
      481 aattctggaa aagacagcag tattagttat gaaattgctt tcaacaaccc aataagttta
      541 tctctagaat ataataccct tgcaacctca gcagatgttt taacattagc taccgaagtg
      601 agagtttctt gtcctgcaaa tagaacacta accgttctta atccagatga tcagattcta
      661 attgacacca attttgatgg aatatatgaa agtggtgtta cccaattctc ttcttttgaa
      721 attcggtttc gtttaaacgg aagttcggct ttagctcctg gcacaggtac attttcaatt
      781 agaggaaaca ctattactac tctaaaagta actaacatca acattgccga atttggaaaa
      841 agtaaagtag gcttgcacat caccgccact tgtgttgaaa aagattctga caatgatggt
      901 attccagatc aatatgatta tgatagtgat aatgatacga taccagatag aatagaatca
      961 caagggcaaa atatactcgc tttatcgggt attgatgcta acaatgacgg tatcgatgat
     1021 gtttttggaa acggaatact acctgcgaat accgacaatg atactgttcc aaattattta
     1081 gattcagaca gcgacaacga cggaattttt gacattatcg aatcgggaag cactggaaac
     1141 agcaacaata ctagtggaat taccgttggg aatgttggtg ccaatggttt agacaataca
     1201 ctcgaaacat cgcctgatag cggaaccatt aattatacac ttaccgatac ggatggcgat
     1261 ggaatataca actacatgga attagacagt gatgatgatg gctgtaatga tgtaattgaa
     1321 gcaggttttt cagacccaaa taacgacggt attctagggg atgataatcc aactgtattt
     1381 gacactcaat ttggattggt tatttcttca tcaggctatg gagttcccaa tgctaattat
     1441 actattgcag caccaatttc aattagtgca caaccacaaa gtttacaagt ttgtgaattg
     1501 caaacggcaa cttttacagt cacttcttct ccagtcgata gctaccaatg gcaaatctct
     1561 accgatggcg gaataacttg gacaaacgta atcaacaatg ccactaatgc tggtgctact
     1621 acaaatgctt tgacagtttc aactgtagtt cctgcaactc caaaccatca atatcgtgct
     1681 ttcttaaaca gaattggaaa tagttgtgga ttatattgca atgctgttga acttacaaca
     1741 tatgcattac cagtagtaaa tacgcctatc gacttaaaac aatgtgataa cgataccgat
     1801 ggaatttcag ttttcaatct aacacaaaaa aacgatttta tttctgccaa ttatttgaat
     1861 gaaacttttt cttattacac caatcaaacg gctgcaaaca cgcaaaacaa ttctttccta
     1921 atcactaatc caatagcata cacttctgga atagccacag tttatgcaag agttgaaaac
     1981 agcaacggtt gttatagtat tgcaacaata aatctaatcg tttccgttac acaaattcca
     2041 gctaatttcg ccattcctat catgcatgaa tgcgacgatt atattgatgc tataaacgat
     2101 gattttgatg gtatttcggc atttaacttt acaaccattc aaaatagttt acttgctatt
     2161 ttacccacta atgtttctgt ttctttttat aaaaacgaag ctgatttctt ggcagaaacg
     2221 gatagtgatg gaaactcttt agccattaca gacatttcta attatagaaa taccgattac
     2281 cctttccaac aaacgctttg ggttcgtgcc gatagtattt tagacaattc ttgctatggt
     2341 tttaaaactt ttgaaataag agttgtgcct tggccaagac ttgacttatt ggaaactgtt
     2401 ttaatttgtc ttcccgaaac gcaaaccact attaacgctg gaattattga tggatcacca
     2461 acgtcgaact acacctatca gtggtattct aatggaagtc cattaccagg cgaaaccaat
     2521 tattcgctaa ctgtaagctc gccagcaact tattctgtag atgtaaaaac tatatataat
     2581 tgtatcagaa caagagttat tgaagtggtt ggttcccaaa ttgcttcttt actatctatt
     2641 gaaatttcag acttaactca aatcaatacg gtcttaatta acgttactgg ttctgggatt
     2701 tatgaatatt cattagacga tattgctggt ccatatcgaa gttctaattt ctttaccaat
     2761 gtaccaatgg gcttgcacga tgtttatatt agagatacaa acggttgcgg aatccttggc
     2821 ccattgccta tagctgttct tggaattcct cagtatttta ctccaaatgg agatggttat
     2881 aacgatactt ggaatgtaaa aggaataaac caaacaaatg ttcaatccat catttatatt
     2941 tttgaccgat ttggcaaact catcaaacaa atcagtgcag ttagtccagg ttgggacgga
     3001 acttacaacg gacatcaatt gcccgccgat gattattggt acaatatcca atttcaagat
     3061 ggacgaaatg ctagaggtca tttctcttta aaaaggtaaa aaaacactat ttttgttgtc
     3121 ctaaaaaggt gctacaaaac cttcttaggt atttaataaa aatagaatgc tttttaaaag
     3181 atactacatc ttattgcttt tgttttttgg caccgcttct gcacaataca tctctactga
     3241 tgataccaga acgccgttgc aattaataga agatgtatta ataaattcag gatgcgcttc
     3301 cgtgtccaac gtttctgttt caggcggcaa ctttgctact ggagatacta gttatggcta
     3361 ttttaatgcc aacggttcta gctttacatt tcccgaaggc atcattctta gcaccggaaa
     3421 aatcatcgat gcgcgcggac caaattattt catatccgat gatactgcac cgggatggac
     3481 tggcgaccaa gatttaaatt atgctctagg tattagcaat tctataaatg caactgtttt
     3541 agaattcgat tttataccta tcggaaacca aattagtttc gactacattt ttgcttccga
     3601 agaatatcat ggaagtgcca cttgcaccta ttctgatggt tttgcatttc ttttaaaaga
     3661 agtaggaaca accaattatc aaaaccttgc cctaattcca aacacggcta ttcctgtaaa
     3721 agtaactaca gttcaccccg aaattcttgg tggttgtcct gcgcaaaacg aacaatattt
     3781 tgatgccttt aatggtcctg aatatccaac taatttcaac ggacaaacca aagtattaac
     3841 tgcccgagca actgttattc caaacacgcc ctaccatatc aaattggtca ttgccgacga
     3901 aggaaactac cgctatgatt cggcaatttt cctaagaggc ggcagtttca atcttggtct
     3961 tgacttgggc gatgaccgaa ccttagccaa ccgaaaccca gtttgttttg atgaaaattt
     4021 aaccctaaac gcaacttctg ttggtgccca aagttaccaa tggaacttca atggaagccc
     4081 aatagtcgga caaaccgcaa gcacattaac ctttaatcca ccatattcta caacacaaaa
     4141 cggaatatat gctgtggcag ttacttattc gctaacatgt accattgttt cggatattga
     4201 gttagaattt tcacccgaat tacaagccaa tcaaacgtcc tataattttt gtgataccga
     4261 aggagcgcaa gacggcatat ctaccattac tttgagcgat atcacgccta cattattcac
     4321 caatttgcct gctaattttc aagtaaatta ttttagcagt ataacaagta ccactccgct
     4381 atctaattta tttacgttaa caacgccttt tcaacaaatt atttatgttc gaatagccaa
     4441 taataattgc tacaacaaca ttcctgttac actaaatatt caagttttta ccgataccat
     4501 tttggacgaa actcttggca tttgcaacaa cgacccaact atactgactg ctgatagcgg
     4561 ctatccgtcc tacctttgga acactggcga aacatctcca tccattcaag taactatgcc
     4621 cgacacctat accgttacta ttggaaacaa tgacggttgc accaaagtaa aaacatttac
     4681 cgtcgttggt tccgaaattg ctacgataac cgatataatt atcaacgatt ttaacgataa
     4741 caattccatc gaaatcattg caataggcaa tggagattat gagtattcta tcgatggagt
     4801 tcactatcaa tcctcgacta ttttttataa tctaagcaaa ttagaataca ccgcctatgt
     4861 gaaagacaaa aaccgttgtg gcattgctac tcatgtgttt tatctactgc aaatacccaa
     4921 atactttact ccaaacggcg atggttttaa tgaaacttgg aacattagaa acctatccga
     4981 aagaggattt aaccaaacca tcatcagcgt tttcgacaga tatggcaaac tcctaaaaca
     5041 attcagcgga aacagcaccg gttgggacgg aacttataac ggtaaaaatc ttttggcaga
     5101 tgattactgg ttttcaattt ctaatcaagg caaagaactt tttcgaggtc attttacact
     5161 caaacgataa acctcaaacc tcactaaatt accattacta taaaagaaga ataactaaaa
     5221 aatgttctat aattctaata gtcaaacaaa aagttatcgt tttttaataa ctaaacaact
     5281 tatttaaata atcggaagat taattccaac atgcggaaat tcattttgaa taagtataaa
     5341 attaattcac atcaacggta actttattcc tgcttacaga agttttattc caacctacgg
     5401 aaatttcatt ctcgtttacc gaagctttat tcccacctac ggaaatagta ttctgccata
     5461 cggaagaaca attttcatca gcgtaaacac cttttttact gttattttat aacataaaac
     5521 aaaaaaaccc tttccataaa gaaagggttt catttataaa tctaaaacga ttatttatat
     5581 tttgttacgt tttctatcat tctcatctaa gaaaatctta cgcaaacgaa tgtttttcgg
     5641 agtaacctca acatactcat ctttctgaat gtattccaat gcttcttcca aagaaaaacg
     5701 tttcgcagga ataattctag ctttatcatc agcacccgaa gaacgaacgt tagacaattt
     5761 tttagtctta gtaacattca cagccatatc atccgaacgt gtgttttctc caattacttg
     5821 tcctgcatag atttcctcat ttgggtcaac aaagaaagta cctctatctt gcagcttatc
     5881 tattgagtaa ggaatcgctt ttccgttctc catagaaatt aacgaaccat tatttcttcc
     5941 tggaatttct cctttataag gctcatatcc aataaatcgg tgagccatga tagcctcacc
     6001 agcagtagcc gtaagcaatt ggtttctcaa tccaataatt ccacgagatg gaatattaaa
     6061 tttaacaacc atacgctcac cttttccttc catcgaaagc atttctcctt tacgaaccga
     6121 aacaaactct actgctctac ctgataaatt ttctggtaag tcgatagtca actcttcaat
     6181 tggctcacat tttacaccat caacttcttt gatgattact tgtggttgtc caatttgcaa
     6241 ttcgtaacct tctctacgca tcgtttcaat taaaaccgac aagtgaagta caccacgacc
     6301 aaataccata aacttatctg cagaatccgt ttcacctact ttcatagcta agtttttctc
     6361 caattctttt gtcagtctat ctctaatatg tcttgaggtt acaaactttc cttctttacc
     6421 aaagaatggc gaatcgttaa tagtaaacaa catactcatt gttggttcat caatagcaat
     6481 agtttgtaat gcctcagggt tttcaaaatc ggcaatagta tccccaattt caaaaccttc
     6541 aactccaact atggcacaaa tatctccagc aataacttct tgtacttttt tacgacctaa
     6601 accttcgaaa gtatgtaatt ctttaattct agatttgata acttttccgt ctctttttac
     6661 taatgaaatt ggcattcctt ctcgtaaaac acctctttct aaacgcccaa tcgcgatacg
     6721 accagtaaat gaagagaaat ctaaagaagt aatcaacatt tgtggtgttc cttctgatac
     6781 ttttggtgca ggcacattgg ctacaaccat atctaataaa ggttcaatat tctccgtttg
     6841 atttttccaa tcatcactca tccagttatt tttagccgaa ccatacacag ttgggaaatc
     6901 caactgccat tcttcagcac ctaattcaaa cattaggtcg aaaactttct catgaacttc
     6961 ttcaggagta caattttctt tatccacttt gttaataacc acacaaggtt ttaaacctaa
     7021 gtcaatggct ttttgtaata caaaacgggt ttgaggcatc ggtccttcaa aggcatcaac
     7081 caaaagacat acgccatcag ccatgtttaa tacacgttca acctcaccac caaaatcggc
     7141 gtgacctggc gtgtcaataa tgttgatttt tgttccttta tatactacag aaacattctt
     7201 tgaagtaatc gtaattcctc tctcacgctc caaatcatta ttatcaagaa ttaaatcgcc
     7261 agtattttca ttttcgcgaa ataattgaca atggtacata attttgtcaa ccaatgtagt
     7321 tttaccgtgg tcaacgtgag caataattgc gatgtttcta atagattcca taagattttt
     7381 tttcaaggcg caaaggtaca cttttatttg gtaaattaat caacaaatgt tggtatgaaa
     7441 aaaatagaaa ttcattaata agtttttagt taaaaataca tccacctttt aatttattta
     7501 tatatttgaa ttaatgaaaa gtaattcgaa caaaactacc tttatttttt tattactctt
     7561 gtcttttagt gcattaggac tatctttttc actttcatac tttaataatg gtactattaa
     7621 tactgatttg ttatttgtag aaaatataat tttaatagta atcgcaggtg ctatatttaa
     7681 atacattact catagaaaca aactgagaaa caaagcagag tttgaaaact taataaatgt
     7741 caacaaagat attattgaag ccaaggagcg ttatgatatt gtggccaaag caaccagtga
     7801 taccatttgg gattggcgca ttgaagaaga cattatgata tggaacaaag gcatacaagg
     7861 tgtttttggt tatgataaaa aagaaattga aaaatcttca aaatggtggt ttgaaagaat
     7921 tcatccagaa gacagtttaa aaatgtcggt taggctctac tcttttttgg attttgtaaa
     7981 agaccgttgg caagatgagt atcgctttag atgtgcagac ggaacataca aacatgtgat
     8041 agacagaggt tttttaataa aagacaaaag tggtaaaatc gttagaatga taggtgccat
     8101 tcaagatgtt agcaaagaaa aagaagaaga agaacgatta cgtcttcttg aaaccgtaat
     8161 tactaatact aaaatcgcta ttgctattgc aaacatagaa cttaacgaaa cgcaatatcc
     8221 aaaaatatgg tatgtcaatc ctgccttttc agagataact ggttttagtc gagaagaagt
     8281 tgtatcaaaa gattttagca ttttattcaa caaaaacacg gatgttttac aactagaaaa
     8341 cttaaaaaat gctattaaag aatatagaga atgtaccttt gaaaccgctt gtagtaaaaa
     8401 ggacggcact cctttttgga taagtctttc gatgattcct gtaaatgatt ttgaaggaag
     8461 gcattcgcat tggatttcca ttcaaagaga cattactatt gaaaaagaaa aaataaaaga
     8521 aagagaacaa ctcattaaag aattgaccaa aaacaataat gatttaaagc aattctcata
     8581 cattacctct cacaacttaa gagcaccgct atcaaattta actggattac tcaatttaat
     8641 tgaagacatt cctatagaaa acgaagactt aaaggaaatc attgatggat ttgccaactc
     8701 aacctatatg ttaaatgaaa ccattaatga tttagcaaaa gtcatcatca ttaaagacag
     8761 ctctgcaata aaaaaagaaa aaatattagt aaaagatgtt tttgaaaatg tctttaacca
     8821 agtgtcgttc ttaatcaata ctataaaacc gattttaaaa atagatttgg aagaaactta
     8881 tgttttgaat agtaataaat cttatttaga gagtattttt ctaaatttaa tgactaattc
     8941 attaaaatat aaatcgacta agagacaact taaaataaat gtaacctcaa aagtagaagg
     9001 cgatgaagtt atcattgttt ttgaggataa cggaatagga attgacatgg aaagaaataa
     9061 aaacaagata tttggtttgt accaaagatt tcataatctt ccagacagca aaggcttggg
     9121 gctgtattta gtcaaatcgc aagtagaagc tatggatgga acaattagtg tagaaagtga
     9181 agttgagaaa ggaacaactt ttataataaa atttaaaaat caataaatgt tacataaagt
     9241 tttatgtgta gatgatgacc caataacctt gatgctttgc aaaaaagtta ttgaaagagt
     9301 aaattacacc aaagaggttg ataccgcaaa aaatggcgaa gatgctattg cttattttaa
     9361 tcaattaaaa aacaaccaag aaattcctcc cgaattagtt ctactagatt taaacatgcc
     9421 aataatgaat ggttgggaat tattagacgc ttatctagaa ttagaattta atttaatttt
     9481 caaagaaaca aaatttatcg tgttatcatc aacaatagac ccaagtgata ttgagaaatc
     9541 aaagagttat ccaatggtaa ttgattttat atcaaaacct attactaaag aaattttagc
     9601 agaaataaaa ctataaaaaa agcactcaaa attgagtgct ttttatatat aataaactta
     9661 aacttaatta aagcttagct acatgtttcg ttaacttaga ttttaaatta gcagctttgt
     9721 tatcatgaat aacatttttc ttagccaatt tatcaatcat tgaaatcaca gcgttcaact
     9781 tagcagatgc atctgacttg tctgttgcta ttctcaatgc ttttatagca ttacgagtcg
     9841 ttttgtgttg gtatttgttt aatactcttc ttttctcgtt acttctaatt ctttttaaag
     9901 ctgacttgtg atttgccatt tttgtttttt tctattaaat aaaatttgta gcccgtaggg
     9961 gaatcgaacc cctcttacca ggatgaaaac ctggcgtcct aaccgataga cgaacgggcc
    10021 atctttctca atgcggatgc aaaaatacaa ctatttttta ttttcgcaag agttgcgata
    10081 ttttttttta aaaaaaattt aatatgcttt tgcaaacagc actctaccaa cagattcctt
    10141 tccagaaaaa acacaaattc ctttttctaa ttttctattt aagggaatac accgaatagt
    10201 tgctttggtt aattctttga ttttttcttc cgtttcacca gttccatccc aatgagcagc
    10261 aacaaatcct gctttatttt ctaaaacttc tttaaactct tcaaaagaat tcacctcagt
    10321 gatatgtgaa tttctatact ctaatgcttt ttcaaacaac tctttttgaa tcgtttctaa
    10381 taattccgaa atataagcta caattccttc tttttcttta acttcttttg ataaagtatc
    10441 tcttctagcg atttcaaaag taccattttc taaatctttt ggaccaattg caattcgaac
    10501 tggtactcct ttcaattccc attctgcaaa cttgaaacct ggtttgtgcg tggttctatt
    10561 atcaaattta actgaaacat ttgctttttt aaactgagaa accaactcat tggcaacttc
    10621 tgttatttga tgaaactctt catcagtttt aaaaatagga acaattacaa cttgaattgg
    10681 ggctaaattt ggaggcaaca ctaacccatt atcatcggaa tgtgtcatga ctaaagctcc
    10741 cattaatcgt gttgatactc cccaagatgt tccccaaaca tgctcttgtt ttccttcttg
    10801 atttgcaaat tttacatcaa aagctttggc aaaattctgt ccaagaaaat gagatgttcc
    10861 tgcttgcaaa gcttttccat cttgcattaa ggcttcaatg caataggtct catcagcacc
    10921 tgcaaacctc tcattttctg ttttaattcc tttcacaact ggaattgcca taaaattttc
    10981 tgcaaaatca gcataaacat tcatcatttg aatcgtttcg tctattgctt cttgttttgt
    11041 agcatgagct gtgtgtcctt cttgccataa aaattcagcc gttctaagaa acaatctagt
    11101 tctcatttcc catcgaacaa catttgccca ttggtttata agtagcggca aatctctgta
    11161 ggattgcacc caagttttat aggtactcca aataatagct tcactcgttg gtctaacgat
    11221 taactcttct tccagctttg catttggatc aaccatcaac tttcctttat tgtctggatc
    11281 gttttttaat ctataatggg ttacaatagc acattctttg gcgaaacctt cggcgttttt
    11341 ttcttcggcc tcaaacatac tttttggcac aaaaagcgga aaataagcat tactatgtcc
    11401 agtttcttta aacatcctat caagttctgc ttgcattttt tcccaaatag cataaccata
    11461 aggcttaata accatgcaac ctcgaacacc tgaattttcg gctaaatcgg ctttaacaac
    11521 caattcgtta taccattttg aataatcttc ttctcttttc gttaagtttt tgctcatctt
    11581 gaatattttg gcacaaaaat tgttaatctt tttttaacta aattattcag caaaactaac
    11641 tatttttgta tagtccaaca ataaaaaatg ctactgttat gaaaactatt ctacaattta
    11701 ctcaaaaaaa ctcctattat ctaataatag gtagtcttct atttttaatt tcttcttgtg
    11761 gttcttatca aaacacttct tattacgaca atgatggtgt ttattcaagt agaactaata
    11821 caactacaac agaaaacaat tcaaaagcta agagtaatca ataccaagaa tactttagtt
    11881 ctctaaatca agataataac gaattttttg tagatgttga taattattca tctgtacaaa
    11941 atgataccgt tacccaaaac aacaataatg ctaccgaaag ttattcaggt tggggaaata
    12001 atcctcaaac agtagttgtc aatgtttatg ataataattg gggttatggc tattggaata
    12061 actattggta tggtaattat tgggtaaata atggttggaa ttggggaatt ggttggaata
    12121 gttggtatgg tcctagcatt ggttggggtt ggaataattg gtatggaaat tactggggtt
    12181 ggaatggttg gtatggaaat tattggggtt acaactatgg ttattctcct tattggggtt
    12241 ataatcacgg acattatgcc aactggaatg gtggaacaag aggaaataat tacaactatt
    12301 acaacggaag tagaggtaga aattcttata ctactggaag aaatagtata actaacggca
    12361 ccagaaacaa cagttttggt acttcaagaa ataacacaag aggaaattcc tattcaacaa
    12421 ctcgaaatac tacatacagt aatagtgcca atgtaagaac aaacaattat aataatacaa
    12481 gaggaaattc aacttctaca aattcttata attctactac aagaagtaat aattctacta
    12541 gaactaacac ttcatcgtca cctgttcgct caaatactca aagttcaact aattatggtg
    12601 gaacacgttc ttcctctgga agttccacta gtggaagctc aggcggcgga agatcttctg
    12661 gtggtagaag ataatttaga tatacaaaaa tttataaaaa aagctatgaa aattatttat
    12721 acatttatgt tttttggtct tactgttttc acaataaaag ctcaagaaac aactattaac
    12781 gatgcgctaa ggttggccgt tgacaatatt acaggaacag caagataccg ttcgatgagt
    12841 ggtgcttttg gagctgttgg cggagactta tcgtcaataa atcaaaatcc tgcaggttct
    12901 atttttttca ataataacct agcaacgggt tctatatcct attttggggt taggaataaa
    12961 tccaactatt ttgggacaca aaccactgaa aatgacaact ctctagattt gaatcaagcg
    13021 ggtgtggtat ttgtttttaa cgaaacgagt aaatcaacag attggaataa aattagttta
    13081 gcattcaatt atgaaaatac taataatttc aacaacacta ttttttcagc cggaacaaat
    13141 ccttataatt ctattggaaa ttatttttta gactttgcgc aaggaattcc tttagattat
    13201 ctgaaaaaca ttaactttaa tcaactgtat tttaatgagc aacaagccta tttaggttat
    13261 gatacttata tttttgaacc atttactaat gcatcagaca acgtagatta ttatacaaac
    13321 attccaactg gaggaaatta ttaccaagaa aattatacta atactaatgg ttataacgga
    13381 aagctaacag caaactttgc aacttcctat aaaaacaaac tatttttagg tgcaaaccta
    13441 aatgttcatt ttgttgatat tcaaaaagta tttaacatat atgagagcaa taataatccg
    13501 gtttatgatg aaggttccac aattggaagc attttcttta gaaacgaatt attcaccttt
    13561 ggttctggta tttcttttaa tcttggagcc atttaccaac ctatagacaa tataagagta
    13621 ggagttgctt atgaatcgcc aacatggtat cgattgacag accaattgac acaaggtgtt
    13681 gttacaacaa gtatcaataa ccaagacaac aattcaagac catcttcttt tcaagacaca
    13741 caagaatttc taccctatta tattcaaaca cctagcaaat ggacgggcag tttggcctat
    13801 atttttggaa aacgcggact tataagtgcc gatgtttcta tgaaagatta ttctgtaacc
    13861 cgttttaaac caaaaaatag tcaaccttat acgtctttga atacaacaat ggactcaatt
    13921 ttagacaatg caatggaggt acgactcggt ggtgaatata aaatcaaaca atttgcaatt
    13981 cgtggtggtt atcgttttga gcaaagtcca tataaaattg accaagcatt tggtgattta
    14041 acaggttatt ctggtggaat tggctatact tttggagaaa acagaattga cttggcttat
    14101 acgcacgaat acagaaacat gaatcaagcc tttttatcat caggaatgac tgatatggct
    14161 agaataaata gaaaaaacaa tacagtaact gtgagttaca ctattaattt ttaattaaat
    14221 aaaccagaat ctaattaagc cattccatta tttgggatgg tttttatttt tgaataaata
    14281 gaaataaata gtgtaatttt gcactccaat tgatataaaa tgagaacaaa gtctttaaag
    14341 aaaaacaaaa tcaatgtaat aacgcttggt tgctccaaaa acacttatga tagtgaagtg
    14401 ttgatgggac aattaaaagc cagcggtaaa gacgtagttc atgaacaaga aggaaatata
    14461 gttgtaatta atacttgtgg atttattgat aatgcaaaag cagagtcggt aaatacaatt
    14521 cttgaatatg cagataaaaa agaacgtgga attgttgaca aagtattcgt taccggctgt
    14581 ttgtcagaaa gatacagacc cgatttagag aaagaaattc caaatgttga ccaatatttt
    14641 ggaactaccg aattaccttt attattaaaa gcacttggag ccgattataa acacgaatta
    14701 cttggagaac gaattacgac aactcctaaa aattatgcgt acttaaaaat tgccgaaggt
    14761 tgcgacagac cttgtagttt ttgtgcaatt cctttaatga gaggaaaaca tgtttctcaa
    14821 ccaatagaaa aattagtaaa agaagcggaa ggtttagcca aaaacggtgt aaaagaatta
    14881 attcttatcg ctcaagattt aacttattac ggtttagatt tatacaaaaa aagaaattta
    14941 gctgaattac tggaaaactt agtgaaagtg gaaggaattg agtggattcg tttgcattat
    15001 gcatttccta ctggttttcc aatggatgtt ttggaattaa tgaaaagaga accaaaaatt
    15061 tgtaattaca tagacattcc tttacagcat atttcagata acattctaaa gtcaatgcgc
    15121 agaggaacaa cttatgcaaa aactacgcaa cttttaaaag atttccgcgc tgcagtacct
    15181 gaaatggcaa ttagaacaac attaattgtt ggttatccgg gtgaaacaga agaagatttt
    15241 gaaattttga aaaattgggt tcaagaaatg aaatttgaaa gattgggttg ttttgcctat
    15301 tctcatgaag aaaacacaca tgcttattta ttggaagacg atgttcctga agaaataaaa
    15361 caagctcgtg ctatggaaat tatggatatt caatcgcaaa tttcttggga tttaaatcaa
    15421 gagaaaattg gacaaactta taaatgtatt attgatagaa aagaaggtca atattttgtg
    15481 ggaagaaccg aatttgatag tccagatgtc gataatgaag tgttgattga tgcttcaaaa
    15541 ttctatttaa aaactggtga ttttactaat attaaaatta ctgacgctac agaatttgac
    15601 ttatatggcg aacctgaaaa caactaacat tatgaaaaaa atacttttaa ttttattttt
    15661 atcactatca ctgttttcct tttcacaaat ggatagaaga attggaagaa taccacaatc
    15721 tacttcaacc aaaaagaaag aaaaaataga ccctattcaa ctatctttag aatatttaaa
    15781 gaaagaattg aatctagatt cgtttcaaga agcagcgaca aaaacctatt tagaagaaaa
    15841 tcaaaaagaa aaagagtata ttttaacttt ggatattatt gataatgata aagttgaaaa
    15901 acttaaagtt tcttatgaaa aaatggacgc tcaaattgat gctttgctaa ataaaaaaca
    15961 aagagaagct tttataaaat taaaagaaaa aagaaacggt aaagacaaaa agaataaaaa
    16021 agacaacaaa gaagaagaat tagaaaacca atagacattt tctaattctt taaatccttt
    16081 ttaaaaaata actattcttc tacaaagaaa gtttttatac tcttacaaag gaatattacc
    16141 atgtttccgt tttggtgtat cgaccacttt gttttcaagc atactaaaag cttttattaa
    16201 ttttctacgg gtttggctag gcaaaattac ctcatcaata aatcctcgtt gagcagctgt
    16261 ataaggattg gcaaataatt cagcatattc cgcttctttt tctgctaatt tttgtgctgg
    16321 attttcagct tcattaattt cttttttgaa aataatttcc gaggctcctt ttgctcccat
    16381 tactgcaatt tcagcacttg gccatgcaaa attcatgtcg gcaccaatgt gttttgaatt
    16441 cattacatca taagcacctc cataagcttt tctagtaatt acagtgattc ttggaacagt
    16501 tgcttcacta aaagcatata ataatttggc accatggacg ataattccat tccattcttg
    16561 atcggttcct ggtaaaaatc ctggcacatc ttctaatact aataatggaa tattgaatgc
    16621 atcacaaaaa cgaacaaaac gagctgcttt ggtggaacta tttacatcca aacaaccagc
    16681 aagaaccatg ggttgatttg ctactattcc gatacttctt ccgcccaaac gagcgaaacc
    16741 aacgatgatg ttttcggcaa aatctttatg aatttcatag aagctatctt cgtcaataat
    16801 atttccaatt acatcgtgca tatcatatgg cttattagca ttatcgggca taattgtatc
    16861 caatttttct cgtagttcat cctcaaaaac ataaggtaat tttggaggaa tttctctatt
    16921 attttgaggc aaatagctta acaaacgttt aatatcttcc aaacactcta caccatttgg
    16981 agacgtaata tgtgccactc ctgattttgt ggaatgtgta cttgcgccgc ctaattcttc
    17041 tgaggttact tcttcgtttg ttactgtttt tacaacgttt ggtcccgtaa caaacatata
    17101 actatttccc tccaccatca tggtaaaatc agtcatcgct ggtgaatata cagcacctcc
    17161 agcacatggt cccataattg cagaaatttg tggaattact cccgaagatt gcacgtttct
    17221 ataaaaaata tcagcatatc ctcctagaga gcgaacacct tcttgaattc tagctcctcc
    17281 agaatcattt aatccaatca ttggcgcacc atttttaagt gccatatcca ttactttaca
    17341 gattttctcc gcatgtgttt ctgataatga accgccaaaa acagtgaaat cttgagcaaa
    17401 aatatagact aatctaccgt ttattgttcc atatcccgtt acaactccat caccataata
    17461 aatctctttt tccattccga aatcggttgt tcgatgagta accagcattc caatttcttc
    17521 aaaagaacct tcatccataa gatattcaac tctttcacga gcagttaatt tctttttagc
    17581 atgatgagca gcgattcttt tttctccacc tcctatttta gctaaagcaa ttttatcatg
    17641 tactaatttt attttgtctt gcattttttt aattctttgt ttagggtttt ggtcttacaa
    17701 cttaattcta aaatcttact gctcttgatt aggtaatcgc acgatttttt tatcgtccaa
    17761 atactgttgt aaagcaatca tcgctgcaat ttcggcttca gtgtctaatt ttgctttcat
    17821 cttttcagga ttataatacg cttttacaaa accagtatca aaatcaccag aacgaaatgc
    17881 ttcatgctcc atcacaaatt tcccaaaggg taatgtagtt gctacgcctt ccacatgata
    17941 attctcaatg gctttaatca tcaacgcaat ggcttcttct cttgttttcc cataggtaac
    18001 taatttagaa agcattgggt cataataaat aggcacatcc attccttctg cgatgccatt
    18061 atcaacacga attccttcac cttcgggcaa tttataagtg tttaaaaaac caacgtttgg
    18121 caaaaaatca ttcagagcat cttcggcata aactcggagt tccatggcat gtcctttgat
    18181 tttcaaatct tcttgtttca tatctaaagc ctcacctcga gcgacacgaa tttgcaattc
    18241 taccaaatct aaacctgaaa tcatttcagt tacaggatgt tcaacttgca aacgggtatt
    18301 catttctaaa aaataaaaat taagatcagc atccatcaaa aactcgaccg ttcctgctcc
    18361 aaggtaatcg cacgcacgag ctacttttac ggcagcttct cccatctctt tccttttttt
    18421 gggcgttaaa atacaagaag gtgcttcttc caccactttt tgatgacgac gctgaatact
    18481 acactctctt tcaaacacat ataacacatt accatgactg tcagccataa tttgaatctc
    18541 gatatgtctt ggttttgtta catatttttc aataaaaaca gctccatcac cgaaagcatt
    18601 gagagcctcg gaaattgctc tattcatttg cgatgtgaaa tcagcttctt tttcaacaat
    18661 tcgcattcct tttccaccgc cacctgcggc agctttaatc aaaattggaa atccgatttg
    18721 tgttgctatt tctttagctt cttcaatatc tgaaatagca tcttctgttc caggaaccat
    18781 tggaatatcg taatctttta cagcttcttt agcggctaat ttattaccca tcatttcaat
    18841 agctttggat tttggtccaa taaaaataat gttatttttt tcgcaaagtt cagcaaattg
    18901 agcattttcg ctcaaaaaac catagcccgg atggactgcg tcaacggcta aatttttaca
    18961 aacttcaata attttatcgc ctcttaagta ggattgactg gaagctgctt ctccaatcca
    19021 aacggcttca tcagcatatt ttacatgcaa tgcatttcta tccgcagtag aatatactgc
    19081 aaccgtttta atgcccattt ttttggcagt tttcattact ctaatggcaa tttcaccccg
    19141 attggcaact aatatttttg ttatttcttt tatcatttta ttttttttga cctcaaattc
    19201 acaaattaat ttaattatgt aatgtttcaa tttttcgaat tattctgaaa actaataact
    19261 gaacattgaa ctctaattac tcaaactcaa ttaacaattg tcctttttct actgcatttc
    19321 ctttttcaac cgcaattgat tttatgattc catctcttgg tgaaaggaaa ctgttttcca
    19381 ttttcatggc ttccaaaatt aataaaggtt cattttcttt aacagtttgc ccaactgaaa
    19441 cactaacctc aagtattaac ccaggcattg gtgctttaat agcgttaacc acttttgttt
    19501 taccacgttc aattcctaaa ctactaatta attggtctaa atcatttgaa atagcaactt
    19561 cataagtatt attatttact ttgatagtat atgtttttgc caaaaaattg gtagtgataa
    19621 tttctacttg aaaaggttga ttttctttta gtacatgaaa ctttgttttt ccgtctcgaa
    19681 ctgcgtcaag atgtttaagt tcgatttcat ctaatataaa atgtattgaa ttgtttactg
    19741 aaagtttgta ttttgaaggc ataattttca attaaattgg ttataaattt aagaaaatga
    19801 aatcgattgt gtggtgtcaa aaatcaatta tcttttaatt ttataaaaaa ttataaaact
    19861 aatagtccag aatctcttag tgtaactatt ggtttggaat accaaaaaag ttcaaaatct
    19921 tgcatggttg tttcaaaatc agattttaat tgactgaacg tcattgaaaa catattctct
    19981 atcgaaattt tttctaaatt ttttttcaac caaaatgctt tttctgattc tagatttatt
    20041 tcaatcgttt tgattttttg ataaaaagtc attttagtca tttgccaagt cgttcctttt
    20101 tttgattttg tataatgctc taaaatgggc tttcctccta tccaaaatac ttttgccgtt
    20161 ggtttaactg aaaaattagc ttctttttct aaacaagaaa tgataaaatc gggagcaatt
    20221 tttgttcttg gaattttaaa atcaaaccaa tcttgcaact cgtattcaaa acaaatacca
    20281 tgcatataat tgaacaatga tttctttaat ccaaaactaa atttttcatg attaatacct
    20341 gttttgtctt tgaattcaat atcattgttt gcaaaaagta cctctccaaa ggatgtgttt
    20401 tttggaatta ctccaaattc ttctgggttt aatccaattg gtgaatgcgc tgtcatggcg
    20461 aactgatgcc aaaatcctga ttgtagaacg cctacttcaa ataattgacg caccatttct
    20521 aggctatcaa tcgtttcttg aatggtttga gttggataac catacatcaa ataagcatgc
    20581 accatgattc cagactcggt taagttgcga gttacatgtg ccacttgctc gacagttact
    20641 ccttttttga ttaattccaa taatctatcc gaggcaactt ccaatcctcc agaaacggct
    20701 atgcatcctg attcttttaa aagaacacac aaatcatgag taaaactttt ttcaaatcga
    20761 atgttggtcc accaggtaac tattaatttt cttcggataa tttctaaagc aacttcacgc
    20821 attaatgctg gcggtgctgc ttcatctaca aaatgaaatc cgttttcacc agtttgtgct
    20881 atcaattctt ccatcctatt gaccaatgtt ttggctgcaa taggttcata gagttttata
    20941 taatctaacg aaatgtcaca aaaagtacat tttccccaat agcaaccgtg cgccatggtg
    21001 agtttgttcc atcgtccgtc gctccataaa ctatgcattg gattagctat ttctattact
    21061 gaaatgtatt ggtctaataa taaatcggag taatcaggtg ttccaatttc gctttgtttg
    21121 tagtcgtttt ttgtagaatt atttttataa attacttcat tattttcaag gagaaatgtt
    21181 cttttaaatt cagattttga agcactttga ctactctcag tgagacatac attttgattc
    21241 aataattcta ctggaagctc accgtcatct aaggttatga aatcaaaaaa ttcgaaaacc
    21301 cgtttgtctt ttagacttct aagttctgtg tttggaaaac caccacccat tgctattttt
    21361 attgttggat gatatttttt aatccattgt gcgcaacgga atgcactgta taaatttcct
    21421 ggaaatggaa cggaaagtaa tactaatttg gggcgtactt cttctattct ttcctttaga
    21481 atttttaaag taatcccatc tatataagta aaatcttctt gtagttttga gtataattca
    21541 tcaaaggaat tggcacttct tcctaaacgc tctgcatacc gactaaaacc gaagttttca
    21601 tcgactattt ccactatgaa atcggaaagg tcttctagat ataaagttgc aaaatgtttg
    21661 gctttatctt gcatccccat gattccgaat gcccattcca tatcgtctaa ttggttaaat
    21721 cttgatgatt ctggaagaaa atttctactg caaatttgcc tagccaacga tggatttttt
    21781 ccttgaagaa atgtaattac attgtcaatg gttttgatgt attcctcgcg taaactatat
    21841 attctttggg agttggaagt tgtagtgttg gcatctatgc taccctcgat gtgacaaaaa
    21901 acattgttta gtccttcttt tgagaaaagt tggagaatga cttctattcc caaatccatt
    21961 tgaaacgaat cgatttgctt ggtatttaga aaacctttca aatatgccgt tgctggataa
    22021 ggcgtattga gttgtgtgaa cggtggtgtt attagaagaa tgtctttcat tttagtagaa
    22081 gaaaaacaaa agtaagctat ttaaaaaaag agattctatt tattttccca tttttttacg
    22141 ttctaaataa tattccataa actttttccc gtatagtgtt tttgctactc gtggagtcat
    22201 tgttttttgg atagtgtcga gaaatttgaa attgatatca taaatttcgg ctaaggttac
    22261 ataaggagca acttcggcat caggatgatt gacagcaaaa ttagcagtat ataaatattt
    22321 gctccttttt attgcgtctt gttttttttg aatttcttca atttctttac tatttttatt
    22381 ctttaatgct tcaaatcgtt ttgttattaa gtctaaatct ttatcaacat aacgcgatat
    22441 tacttttgta tattcgtcat aaagttcttg attttttgaa cctgtgattt tgacatcagc
    22501 agtaaaaaaa tctaaagagg tttctatgtt catttttcct ggttcggcaa aaaaaggtat
    22561 gttgttatcg agcgaatttg ttactccacg gtctaaaaac aagtacaaca tttctggaga
    22621 atctaaatca aattcacttt taaaatttga atcaccacta atagtaatag aatcaattgg
    22681 tattaaaatg gtgtctttta ttcgttggat atatagcttt ccttctttta atcctttgat
    22741 atttcctgtg agtacaaagt ttttggttga aggcgcttct tctttgcaag aaattataaa
    22801 aattacaatg attagagata aaacaatttt acgcattttg atgaattttg tgcaatacta
    22861 ctaaaaaaaa tcctcaaaat tgaacatgcc ccaaaaagtt agacactatt tggggcattt
    22921 tttatggaaa gaaaagtaaa gtatgattat ccttttaacc aagcttgatt taattttgct
    22981 tttgactcat caattagttt ataggcatca atttcttcca ttgttaactt aacttttccg
    23041 ttgatggttt gttcttttcc atctcttttt attttaactg aaatggcatc gtcttggttc
    23101 caattttgac tttccattac catttcgtaa atgttatcga ggttataatt gacattattg
    23161 attgctaaaa tgatatcgtc aggtttgatg tctaaattct tgaaaaaatc atttaattct
    23221 aaattagtgg ctactgcaat ttcttttgta gcaggattta ccttaatata aggtgtttgc
    23281 tctttcaaaa aaggattggc aggaacacta ttttttcctt ttccgacacc cattttggta
    23341 aaatacaatt cgtaatctat cggtgttgtt cctgaaacat gggttattaa aaattgtcca
    23401 acttcggggt aagttaatga tgttattttg gcaaaaagtt cgtcatcatt aaaaggcttg
    23461 tttacaccgt attccttgga tagttttttc attaaatcga gaattcctct ttcaccattg
    23521 cttttttcgc gaatgatgat gtcgatacac attccgatta atgcgccttt ttcatatacg
    23581 tttaaatatt ggtctttgta tggttttaca agcacgtttt tactcatttc ggtaaatgac
    23641 atggtgtcgt caaatcttga tgcattgctt attttaccca acattctttg atagaattcg
    23701 tcttgatcaa ttagaccttg attaatttgg aagaggttgg cgaaatattc ggtaacgcct
    23761 tcatacatcc aaaggtgttc agacatttta gggttattgt aatcaaaata ttgaatttct
    23821 ttagagtgaa tacttaatgg agttacaata tggaaaaact catgtgaaac cacgtcaata
    23881 atttgtttgc ccagcatatc tgctggcatt tcttctggaa aaacaacgct tgtacacgta
    23941 tgatgttcta gagcaccata tcctttggcg tcgcgtttat tcgattcaga taaatagagt
    24001 aaaacggtat attttttgtt atcgtttatt ggtcctaaaa aagttttttg ggcacgcatc
    24061 attttttcta aatcgggtaa caatgatttg gcagtatgca ctccatttgg cgaataaacg
    24121 ctgaacagaa tttccattcc atcaaaggta aatgtttctg aattcgattt acaatacatg
    24181 attggattgt cagtcaaatc gaaataacgt tctacgttga atacatcaat tgttgggctt
    24241 ttatctgcat cgtttaggga tgttgaccca actagatctg atggatgatt gatggttacg
    24301 ctatatttta catcaggatt tccatcgaaa tatccaataa aacaatggtt atttagcatg
    24361 aaatttttac cttttaaaat attggaacca gcaggtgaga aaatttcttc tttgccaaaa
    24421 cctctaccgg tttcggtgtc ataagtgtcg tttacgaaat atacaatttg cgaaagtgtc
    24481 tttgcattgc tgattttcca agtattgtcg tcggtttttt caacttttaa ttctttttta
    24541 ttggtatcaa aagcttttaa ttcgtcgata tatcttccat aattatcagc tgaataggtt
    24601 cctggaactg tctttggaat ttgaaaaatg atttcttctt tggttgtttt ttgtggaatc
    24661 attaccactt ttactctatc gtcttcaatc gtattgagat caattattgc ttttactgga
    24721 gttgcatttt gtttgttttg agcaaataca ataacattcc aattaagcac gattaataaa
    24781 gcagttacaa tatgtttttt cattttaaat taatttttca aataagtaaa caaaaataac
    24841 tattcgttac aattgatttt aattttaaga aaggattaac taaaaaatct atcgtttaga
    24901 aattttattc tgggtaattt gtctttgtaa tctactttta aaacgaccaa aggtattcac
    24961 acgtaacttt gcatgttttg atactctgcc ttgaactctt tttcgccaaa gtttgaaact
    25021 tgaaagtttc aattctctcc gcattatttc gatagtttgt tgttctgaga taccaaactg
    25081 aaaagtaata gcatcaaaag tggttctgtc ttcccatgcc atttcgataa ttcgatctat
    25141 ttcaacttca gtaaaaactt ttttatgcat taaacaaaag tcggaattaa ataaaaaaac
    25201 tcttgttgaa caagagttaa tttttaatca aatttatttt ttatataata ttttccttct
    25261 aaatcttcat caaaatcatc aaattttggt tttatttttg gtttatttcc ttgggctttc
    25321 tttttccaaa gttcaaattt atctttagtc aagcgttttt ttagaatctc gattacttca
    25381 ttttcattta ttccaaattc tgcttttatt gtctcaaaag gttttttttc ctcttgtgcc
    25441 attgttacaa cccgttctaa ttgttcgttg tctagtgtta cttgtctact ttttttcatt
    25501 tctgtaaaag caattttatt tttaattaat ttaacaagtc aatattaaga aaaaaaataa
    25561 ttggttcaca aaatttaatg taatttttat acctaatgga ttgtattttt atttttttga
    25621 aatggaattc gcaacagatt agttaaccta ttgtgctctt cgggcttcat gtgtgtgtca
    25681 actccataaa ttagtttttc tctatccaaa gtcatataag taccaaaaag tctatcccaa
    25741 attgaaaaaa tattaccata attacaatcc gtatatggca aaacataatg atgatgtact
    25801 ttgtgcatgt cgggagaaac tatgaaataa ctcaaaaaaa catccagcct ttttggtaaa
    25861 acaatattcg catgattgaa ttgtgttgcc actacggaca acgtttgata tagaaaaacc
    25921 atccacatag gtgcgccaac tataaacact ccaacgatgg taaaaacaaa tcgtaaaaca
    25981 ctttcgcctg gatgatgacg attggcagtt gtaatatcaa tccatgtatc ggtatgatgt
    26041 ataaggtgaa atcgccataa aaatttgact ttatgttcta ttaaatgtac taaatatgca
    26101 cctattaaat ctaaaagcaa caagcctaca atagcgaaaa accatggatt cattgttggt
    26161 aaccaattca aaattccaaa ttgagtttcg gttgtccaat tggccgtttt gaatataaat
    26221 actgccaaag caaaatttac aataacagtt gttagcgtaa aaaatatatt aattcgagca
    26281 tgttgccatt tgttatagtt gaatttgata aatggaatgg ctgattctat caaccaaaaa
    26341 aaagtaattc cacctgctag aattattgca cggtgagatg aaggaatgga tgaaaaataa
    26401 gaaataataa cattcataag ttataacttt atttcagaat tctaaaggta agatttattc
    26461 gtgaaccaat agattttgct gttttaggaa tttgatgttt ccaaaaatgt tgagtttctc
    26521 ctttcataac caacaaactt ccatgttcga gattgatttt taacatttgg tcattgatta
    26581 gattatgttt caaatgaaac atccgttcag cacctaaact cactgaagct agtattgggt
    26641 ttatccccaa ttctttttca ttatcggcat gccaaccatt gctgtccttt ccatcacgat
    26701 aataattcaa taaaacagtt gtaaattttt catggcatat tttttctacc tcttctttaa
    26761 taaaagttaa gacagcaatc caaggatttg gctgcattat gatattagag tagctatatt
    26821 gttttccatc gttaccaaaa agtgatgtca atctaggttg tggatgtgtt tttccaaaaa
    26881 cagtaatcat atcctgctgc catggaattt cgttttttaa ggtttcaaaa agtttatttg
    26941 cttgtatttc atcgaaaaat tgaccataat attctacttc cgcatttgga atatcgaaaa
    27001 ttaatttatc ttttggaaaa agtgaattca tattataaaa atacagaaaa aaaattcaga
    27061 aataatattc ttttttcttt aaatgaatat ataaatacta atttattctt ctgttttttg
    27121 caaaagcatt ttatagatca aaccaccaac aatagcccct gcaat
//
LOCUS       DAOOBH010000022        26430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_2410_length_26430_cov_7.369858, whole genome shotgun sequence.
ACCESSION   DAOOBH010000022 DAOOBH010000000
VERSION     DAOOBH010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 26430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26430
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2410_length_26430_cov_7.369858"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..734
                     /locus_tag="PK218_04155"
     CDS             69..734
                     /locus_tag="PK218_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPW97734.1"
                     /translation="MDRLLVVSYAKRFSLLQIAGVFETAESALSFLENNPVDIAFLDI
                     EMPGKSGLELRQKALEIPVCVFISSHTESAAETFELQTLDFIVKPFKFPRFEQTVKRI
                     EEFMEIRTKASLFESTIGGDVVFVKTGHEQTKVKLHEIVYLEALKNYTILVTQTQRHC
                     VLSNLGELLQDENFQSFVRVHRSFAVQKHFVDKIGNHEIELRNKVLIPIGRSYKYNVK
                     TII"
     gene            731..2698
                     /locus_tag="PK218_04160"
     CDS             731..2698
                     /locus_tag="PK218_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW97735.1"
                     /translation="MRKLLLFFLLFGHFLVAQIPNSLDSLKVYLDTKPKDTSYVLALN
                     SYAFLNVQEGKYEEAARIIQEMERLSKKLDFGTGFYKSMNMKGVIAYSKQNPEKAMEY
                     FIQCNTIIKKYKLPKKIYQNSLNNIGIIYNQLGDREKATKYALELISFQEKNKLSPLK
                     SSPYTQVGDNLKFYKKFDEALLYFQKALIVEINLKDDSGIAINENRIGTVYEDLKKNQ
                     KAIDHFKKGLTHAERANYKLLQVDLLTNLGRMFQKEKNFITAENMLKKAEKYSIDLEA
                     TQNTKVIYSNLGDLYFEQKNIALAEDYYIKALKLSKEINDQSFLFSANENLATLYEKK
                     GDFRKAYFYKTEANVARDSIFKLKTLENTEDLLRKYQTEKKEQEIALKNVQISNAKKQ
                     KWYFISGLFLLAIIGSLLFYQSRNRKKTNKKLQLLNDELDQANKAKTRFFSILNHDLR
                     APVANLINFLQLQKESPELLDVESTKRMQDKTMSGAENLLNSMEDILQWSKSQMENFK
                     PELKQIAVSSLFSDIQNHFSSFENIEICFENEENITLKTDEHYLKTILRNLTGNAIKA
                     FGSSTTLSKPVILWKAWQHNNQTFLSITDNGKGADLEQFKALYDDKEVVGIKSGLGLH
                     LIRDMAKVIDCEIFVESKKDVGSTFTLFFRA"
     gene            complement(2747..3064)
                     /gene="trxA"
                     /locus_tag="PK218_04165"
     CDS             complement(2747..3064)
                     /gene="trxA"
                     /locus_tag="PK218_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPW97736.1"
                     /translation="MALTITDATFDEVVLKSDKPVVVDFWAAWCGPCRMVGPVIDEVS
                     TEYEGKAVVGKVDVDANQEFAAKYGVRNIPTVLIFQNGEVVGRQVGVSPKKTYTDAID
                     ALL"
     gene            complement(3170..7675)
                     /gene="dnaE"
                     /locus_tag="PK218_04170"
     CDS             complement(3170..7675)
                     /gene="dnaE"
                     /locus_tag="PK218_04170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPW97737.1"
                     /translation="MYLIFDTETTGLPRNWSAPISDTDNWPRCVQIAWQLHDEMGNLI
                     EHQDYLINPEGFNIPFDAEKIHGISTELAQEQGVSLKEVLDKFNIALGKSKFIVGQNL
                     GFDINIMGCEFYRMGIDSPMNEKPVLDTCTEVTAELLKLPGGRGGKFKLPTLTELHEY
                     LFNQKFNEAHNATADVEATTRCFLELIKREIFTKEELDVPADYFREFNEKNPKPIQLI
                     GLKHINLKEASEKIRQQFGEKGNVPISKETIKQNQEQLIDVDFVHLHNHSQFSVLQST
                     VSVQDLVKTAAKNKMPAVALTDIGNMMGAFHFVRDILYHNKSVETKNKTAVENGEEPT
                     EKIIKPIVGCEFFVCENHLDKSRKDNGYQIVLLAKNKKGYHNLAKMSSIAFTQGFYYV
                     PRIDKNIIQQYKEDIIVLSGSLYGEVPNKLLNIGENQAEEALLWWKENFQEDFYIELS
                     RHNQEDENRVNSALIDLANKHNVKTVATNNVFYVNKEDANAHDILLCVRDGEKQSTPI
                     GRGRGFRYGLPNQEYYFKSGDEMKQLFQDYPEAISNIQEVVDKIEIYDLAREVLLPKF
                     DIPTEFLVENDNLDGGKRGENAYLKHLTFEGAKKRYKEITPEIQERLDFELKTIENTG
                     YPGYFLIVQDFIAEARNLGVSVGPGRGSAAGSVVAYCLWITNIDPLKYDLLFERFLNP
                     DRVSMPDIDIDFDDEGRSKVMDYVINKYGSNQVAQIITYGTMAAKSSIRDTARVLDLP
                     LFEADKIAKLVPNLKLDKIFSLDDKALKSALRAEEYDKVKELIELANADDLGSETINQ
                     AKILEGNLRNTGIHACGVIITPSDITNFVPVATAKDSTLYVTQFDNSVVESAGLLKMD
                     FLGLKTLTLIKDTVKLVKYRSGIELNPDEFPIDDVKTYELFQRGETVGIFQYESVGMQ
                     KYLRDLKPTVFGDLIAMNALYRPGPLEYIPSFVKRKNGEEEIVYDLEANEEYLKETYG
                     ITVYQEQVMLLSQKLASFSKGDADVLRKAMGKKQKEVLDKMKSKFISQAMENGHAEDK
                     LEKIWKDWEAFAQYAFNKSHSTCYAWIGYQTAYLKAHYPAEYMAAVLSNNMNDIKQVS
                     FFMEECKRMGLNVLGPDVNESFYKFTVNENYAVRFGMGAIKGVGAPAVDTIVENRKKA
                     KYKSIFDLAKKIDLRAANKKAFESLALAGGFDGFSETHRAQYFHDDGDGITFLEKATR
                     YGSKYQENENSSQVSLFGESSDIQIPEPIVPPCEEWSTMEKLAKEKEVVGIYISGHPL
                     DDFRFEMKYFCNSKLESLKNLDQFVNKSLTFGGIITNVQHRTSKNGKGWGSFTLEGYD
                     ENFEFKIFGEEYLKFRHFLIQNNFTFMKVLIREGWINQEGKRGEPRIQFQTLQYLQDV
                     LPQFAKKLIIAMDIKELHSELIHRLNDLFQSNKGDNLVTFEVVEREIIKIEAKPIDTL
                     TNTSIDIDNNTEEMEFSEENEIEIPEDIKTVTVNKITMPSRKLKIKISHELLSGLEKL
                     QLNFKLN"
     gene            complement(7851..8195)
                     /locus_tag="PK218_04175"
     CDS             complement(7851..8195)
                     /locus_tag="PK218_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPW97738.1"
                     /translation="MNIFVGSLPWSIEEADLRESFEAYGAVESVKVITDKFTGRSKGF
                     GFVEMSNDDEALKAIEELNGATVEGRTIVVNKSEPKPEGERKSFNNNRSGGFDRNNRG
                     GGNYGGGNRERY"
     gene            complement(8329..9042)
                     /locus_tag="PK218_04180"
     CDS             complement(8329..9042)
                     /locus_tag="PK218_04180"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HPW97739.1"
                     /translation="MRKFLSLIAITFLMMSCSEDVKFNDPGLQGLKNDSFWRAADVRA
                     YVTDGKLTIEAYAQYEVLTLGTSSTNLGKYKLGSTNSSNFASYATTFDDVAIEYATIP
                     TPGPVSTVSLTNVGTGYTDATSVATTGGSGSGLSVNIKANANGGVTEVTLLSRGNGYM
                     AGDIVTITGGNLNSKFRVVNVQNSNGEIEITQFDNVKMTISGKFKFNAVNSNNNPLSA
                     DVVNFQSGEFYNVQIYPSI"
     gene            9240..9833
                     /locus_tag="PK218_04185"
     CDS             9240..9833
                     /locus_tag="PK218_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPW97740.1"
                     /translation="MSVKIRLQRHGKKGKPFYWIVAADARSKRDGKFLEKLGTYNPNT
                     NPATIDLNVDGAVQWLHNGAQPTDTARAILSYKGALMKHHLDGGVRKGALTQEQADAK
                     LTQWLEAKSGKVDSKKDGLSKAKDEAKAKALKAEKEANEKRAAKAVEVAEETTEEVVA
                     EEAISVEDAPVAEVVAEEAPAVEETPVAEENNEEKEA"
     gene            9849..10373
                     /gene="rimM"
                     /locus_tag="PK218_04190"
     CDS             9849..10373
                     /gene="rimM"
                     /locus_tag="PK218_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466225.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPW97741.1"
                     /translation="MHKKECFYLGKIAKKFSFKGEVLLYLDTDEPELYENLESVLVEM
                     NKNLIPFFIENSSLHKNDFLRVKFEEVDSEEEADSILGCEIYLPLTMLPKLEGNKFYF
                     HEVIGFKIEDKRLGIVGEIVSINDTTAQPLFEVLNNGVEILIPMIDHFLLEIDRKNKK
                     VVMDLPEGLIEMYL"
     gene            10446..10649
                     /locus_tag="PK218_04195"
     CDS             10446..10649
                     /locus_tag="PK218_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPW97742.1"
                     /translation="MNKPFDLEERTFLFAKECRIYVQKLPKTISNLEDGKQLVRSSGS
                     VGANYIEANEKLGDKDFLFRVKI"
     gene            10849..11556
                     /locus_tag="PK218_04200"
     CDS             10849..11556
                     /locus_tag="PK218_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPW97743.1"
                     /translation="MFSFKKFTVLQDKCAMKVGTDSVLLGAWCPIDNHPFSVLDIGAG
                     TGILSLMLAQRSNAEQIDAIEIDENAYEQCVENFENSPWSDRLFCFHAGLDEFVAEPE
                     EEYDIIISNPPFYSEDFKTDNSQRDLARFQDALPFEELVEAANLLLSENGIFAVIIPF
                     KEEERFIDLCAEVELFPVKVTRVKGTHNTLVVRSLLAFKRYELSVLEADELVIEINRH
                     EYTDDYINLTKEFYLKM"
     gene            11738..12916
                     /locus_tag="PK218_04205"
     CDS             11738..12916
                     /locus_tag="PK218_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPW97744.1"
                     /translation="MKPDLFQSPDYYLLDDLLTEEHKLVRDSARAWVKKEVSPIIEDY
                     AQRAEFPKQIIKGLGEIGGFGPYIPEEYGGAGLDQISYGLIMQEIERGDSGVRSTSSV
                     QSSLVMYPIWKFGTEEQRMKYLPKLATGEFMGCFGLTEPDHGSNPSGMTTNFKDMGDH
                     YLLNGAKMWISNAPFADVAVVWAKNEEGRIHGLIVERGMEGFTTPETHNKWSLRASAT
                     GELIFDNVKVPKENLLPGKSGLGAPMMCLDSARYGIAWGAIGAAMDCYDTALRYAKER
                     IQFDKPIAGTQLQQKKLAEMITEITKAQLLTWRLGVLRNEGKATTAQISMAKRNNVDM
                     AINIAREARQILGGMGITGEYSIMRHSMNLESVITYEGTHDIHLLITGADITGIPAFK
                     "
     gene            13188..13796
                     /locus_tag="PK218_04210"
     CDS             13188..13796
                     /locus_tag="PK218_04210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPW97745.1"
                     /translation="MKNINYLALGDSYTIGQSVCEKCRFPIQLQDSIKKYIDSNDTFS
                     TQIIAQTGWTTTNLKNAIATANPSSNYDFVTLLIGVNNQYQNKPFSIYEQEFPELVNQ
                     AITFAKGNKNNVIIISIPDYAFTPFGNGSMTISNEIDLYNSFAENYCNENNITFVTIT
                     DITRLGLSQPNLVATDGLHPSELAYSKFVERILPIAKMKLGL"
     gene            complement(13797..14129)
                     /locus_tag="PK218_04215"
     CDS             complement(13797..14129)
                     /locus_tag="PK218_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPW97746.1"
                     /translation="MPQDITLKITDRNGQLHEVLAPTDMNLNIMELIRMYELAEEGTV
                     GVCGGMAMCASCQCYVLNDKISLERNSDEEAMLWEAYHVKDNSRLGCQIPITEDLDGL
                     EIEIAPEQ"
     gene            complement(14130..15188)
                     /locus_tag="PK218_04220"
     CDS             complement(14130..15188)
                     /locus_tag="PK218_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW97747.1"
                     /translation="MIETDILIIGAGPTGLFAVFEAGLLKLKCHIIDALPQPGGQLAE
                     LYPKKPIFDIPGYPEVLAGDLVDNLMEQIKQFQPGFTLGETAETITKLEDGTFVVTTN
                     AGTQHKAKAIAIAGGLGTFEPRKPILKDLEFYEKEDRGVDYFIKDPEKYRNKKVVISG
                     GGDSALDWSIFLSNVASEVTLVHRRNEFRGALDSVDKVQELKSEGKIKLVTPAEVIGF
                     KGSERIESVDIEVNGARMNVETDYFIPLFGLTPKLGAIANWGLEIEKNAIKVNNALDY
                     QTNIDGIYAIGDVNIYPGKLKLILCGFHEATLMCQSVYQRINPGKKYVLKYTTVSGID
                     GFDGTRKEAEKAVVKAIE"
     gene            complement(15268..15507)
                     /locus_tag="PK218_04225"
     CDS             complement(15268..15507)
                     /locus_tag="PK218_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPW97748.1"
                     /translation="MTTEELTLNIEKALDEIRPFLNSDGGDIELVSIDEDKHVKVRLQ
                     GACTSCSLSISTMKAGVETTIKKYAPQIETVENIV"
     gene            complement(15509..16642)
                     /locus_tag="PK218_04230"
     CDS             complement(15509..16642)
                     /locus_tag="PK218_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPW97749.1"
                     /translation="MKLDRKEILKALETITIAGEGKNMVESGAVQNVITFGDEVVVDL
                     LLSTPAMHIKKRAEDDIKKLIHEKFSPDAVVKVNIKVEAKENPNEIKGKAIPGISNII
                     AVASGKGGVGKSTVTANLAVTLAKMGFKVGVLDADIYGPSMPIMFDVENEKPISIEVD
                     GKSKMKPIESYEIKLLSIGFFTSPSQAVIWRGPMASKALNQMIFDAAWGELDFMLIDL
                     PPGTGDIHLSIMQSLPITGAVVVSTPQAVALADAKKGVSMFMSDAINVPVLGIIENMA
                     YFTPEELPNNKYYIFGKEGAKNLAEDLNVRFLGEVPLVQSIREAGDYGRPAALQTASS
                     LEKVFEDIARNVVEETVRRNEDLAPTEAIKITTMAGCSAVKGK"
     gene            complement(16783..18078)
                     /locus_tag="PK218_04235"
     CDS             complement(16783..18078)
                     /locus_tag="PK218_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW97750.1"
                     /translation="MNKASFLISLCLFSSFCFAQETNWTLQKCIALGIENNLAIKIQQ
                     LEIKRNQKTKVSMLNELLPQVNLYGNQSYNFGSTIDPSTNGRVSSNIQYDNFYLSAQM
                     NLLDFSKIAEAQKDKITIEIAMAEKEIIENEYKLQVLESFYQTLFTQELQKIQEIQLQ
                     NSKYNLDRISKEVTIGSKPKSDLYDIQFNYAQEEKRFLETQQLLTIQKTQLFQLINYK
                     DLNIETVVLEHTIANPENDLENLKNPKIKAALLALEKSEKEINFQRANNLPTFSTFYQ
                     LASFYYKPLNQPEIVVENFSNQIGNNKNQQVGVQISIPVFNGFKNNRAITAAKIDSEK
                     SKLKIEQEKNSLYQQLELEQKNKKNLESIQAKLIEIENFARASFTTTQAKFTTGVIDV
                     FSYSMAKNNWLTSQYEVIKNKLQLQYIAFKMNLIKTNTL"
     gene            complement(18071..20506)
                     /locus_tag="PK218_04240"
     CDS             complement(18071..20506)
                     /locus_tag="PK218_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW97751.1"
                     /translation="MLKNWTNLFLFHIKNNKLFTFLNVLGLSIGIAGLIFATLYWNDE
                     QSYNNWNPGKDNVFISISKIGIDEYWASNVVPFEEYFKKDFPELESYCYFDNWYEEAV
                     LMYKNKKEIVKFTYAQNSFFDMFPFTIVGGSTKNVLNDNTNIALSQDAARKLFGNENP
                     LGKTLDDGSRKLVVGCVYTIPGKSSMAPEVIVNSIDERMIENKDHWGNYSYGLLLKLK
                     NPDDKDKVIARMERLMHENSTVKNAKQEGISPEEWLKKNGITSTKFYLQSLDEIRLHA
                     ITECFAEGKGNYQFLLIMIGLSILILVLSIVNYVNLATANAVKRAKEVGVRKILGASK
                     SNIIKQFLFETNITVLFSILLSLVIVELSLPYYNDFLGKKLVIFGNQFYMQIVVIFII
                     TIIFAGIFPAIYVSNFETLKVLKGNFGRSKNGIWLRNGMLIVQFAIASFFIIGSYIVY
                     QQIQYVSTKNLGFKGDQVAAVWYWNGYNWREKDYKEKVESRYLLIKNELSKIKGVKQV
                     STGAFEFGSGNGSTSGFSYRGSENIQARNMGVDFGMLEMMQIQLNEGRFLSNKIASDT
                     INSMLVNETALRMMNEKNPIGKEVNWNDKKLKIIGVVKDFHLFGPQENIPPMVFFHYK
                     TVDWMLQNASRIYVKIDAQNPSQTIAEIEKFWTKNVDTVYPFSYDFVDKQYARTYASY
                     VKQKNLFSLLNFIVILIALFGLFALASYSIQRRMKEIAIRKTLGAETNVLLKELSKQY
                     IVFCILGFITALFPVYFLLNKWLENFVFRIDISVLPFIVGFFALLFLTLIVVLSRAYQ
                     ATKVDVLKYLKYE"
     gene            complement(20531..21307)
                     /locus_tag="PK218_04245"
     CDS             complement(20531..21307)
                     /locus_tag="PK218_04245"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HPW97752.1"
                     /translation="MSKIILTLSTIIISLFSFGQVVENREVADFSKLKVSNSIDVHYT
                     ISSTKSLKIETDDEKNLQFIKTETVGETLKIYIENDSYNQKGKNKNWKNKLSFKTLKI
                     NISGPNLDEIKASSSSVVKILNVNESKKLLLTVSSSATIKGNFESDVVTLDTSSSGTF
                     TGEIMAKEIAIETSSSSTVSIEGKAKNVVVKASSSGDCNLKDLKTENATATASSSASI
                     SVYASNSINAKASSSGSISFYGNPSNISKEKSSGGSVIQK"
     gene            complement(21320..>21970)
                     /locus_tag="PK218_04250"
     CDS             complement(21320..>21970)
                     /locus_tag="PK218_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW97753.1"
                     /translation="TKALHEVSITINQGEFVTIMGASGSGKSTLLNIVGLLDSASSGS
                     YQLLSQEIFGLKEREKSKIRKQNIGFVFQNFNLIDELSVFDNIELPLIYNDVSATVRK
                     QKVEKIAERLGISHRLKHYPQQLSGGQQQRVAVARALINDPKIILADEPTGNLDSKNG
                     NEVMELLTDLHAQGATILMVTHSDYDASFSQKTIFMKDGMILSEKNNSKNVDVLIS"
     assembly_gap    21971..22070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22071..23266)
                     /locus_tag="PK218_04255"
     CDS             complement(<22071..23266)
                     /locus_tag="PK218_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW97754.1"
                     /translation="MDTIIKRKNNKKKYGLLALPILLVLGYFVITSLTKKRSLNVNKS
                     EISIKSVEKGFFEDFIVFQAKVTPLNSMLVNVIEGGAVQEIFVNNGDRVSQGQPLARL
                     YNPNTELNYMQQETAIIEQINNLNKAKLDLRNQELNLAKDLISIEHDYQDAKNLYELN
                     QKLFNQEILAKNEWQKTQENFRFQKERMDIIKQSVTKEKQANQIQIGQLNQSISIMNK
                     SLSILRNNKNNFLITAPLSGRLSSFEPILGKTYTQGNSIGTIDVLKGYKLVAEVDEFY
                     LNRIAEGLKGTIEFNEKPVEVIISKVIPEIKSGRFIVELNFIKNENLELNQGLSFGVR
                     INLSEKTESILISKGSFCQETAGKWIFVIKGNKAERRNINIGRENPLYYEILEGLQVG
                     EKVITST"
     gene            23407..24753
                     /locus_tag="PK218_04260"
     CDS             23407..24753
                     /locus_tag="PK218_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPW97755.1"
                     /translation="MKKTTATILVIDDQEDILFTAKMLLKKHFETIYTLNNPKNVIQL
                     LAENQIDLVLLDMNYRIGFEDGKEGLYFLKEIKKLAPNTVVILMTAFGKVETAVEGLK
                     NGAFDYVLKPWDNDKLLETVKKGVGFARKNQKDIILNESTNNYFIGTSKNIEKAYSLA
                     EKVAKTDANVLILGENGTGKYVMAHYIHQNSGRKSNEFIHVDLGSLNENIFESELFGY
                     VKGAFTDAKIDTIGKFEAAQKGTIFLDEIGNVPLHLQSKLLQVIQTKTVTRLGETKPR
                     KIDVRIITATNLDLKAEVAAKNFREDLYYRINTMEIVLPALRERIEDKIPLAHFLLEK
                     MIDKYEKNKIIFDAKALEQIAKHAWNGNIREMENRIERAVILCENNTIKPSDLDLEIV
                     LLHEQHDDFQLSDIEKNTIEKVLLKNENNISKSAEELGLSRAALYRRLEKYNIQTS"
     gene            24759..26087
                     /locus_tag="PK218_04265"
     CDS             24759..26087
                     /locus_tag="PK218_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW97756.1"
                     /translation="MSKFRIYNQLFIRILLVLLGFAVVFYLLNQGLFYSFVFVSFIVI
                     LTNIELYFYLKNAFLFYDKTINAILNNDFSADFSKHKSYKNYNRLFQLYHQLKEKQNE
                     QVSKDIIYRSILNNIETGILILQKEDAEWSIFLMNDFFSKHFEVPKVTKWHYLREHLR
                     SLCELIESQNFSEIKTSLQIRVNQQDTQTFIMQSSRTKMYNQEYYVVLLDSIQKVVEK
                     KEKEAWINLMKVISHELLNSLTPIRSLSQNLHELIQQEHLSQEDLQDIKDSVITMHNR
                     SNHLQEFVESYRKLAMLPSPKKEKIELTTLTENCLQIMKPLFKKEAIEIKNNIDFNRW
                     ILVDPNQMEQVFINLLTNSMFALQDKENKEITISAEHKDKRLYINITDTGNGIESEIE
                     DKIFLPFFTTRKEGAGIGLTLSKNIVEAHGGYLAFKSDENKTQFTVCLVE"
     gene            complement(26129..>26430)
                     /locus_tag="PK218_04270"
     CDS             complement(26129..>26430)
                     /locus_tag="PK218_04270"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPW97757.1"
                     /translation="VYEIARLIPEGKVTSYGAIAKALGAARSARMVGWAMNASHNLED
                     VPAHRVVNRKGLLTGKHHFDGTNLMQQLLENEGIKVIDNQIIDFEKHFWEPKMNL"
BASE COUNT         8708 a         4257 c         4123 g         9242 t
ORIGIN      
        1 tatttatatt tgaaagagaa aatcatttta tgcaagcctt aaactgtatt attgttgatg
       61 atgacgaaat ggatcgacta cttgttgtgt cctatgccaa aaggttttct ttacttcaaa
      121 ttgctggtgt ttttgaaact gcagaaagtg cattatcctt tttagaaaat aatcctgttg
      181 atattgcttt tttagatata gaaatgcctg gtaaatcggg tttagaattg cgtcaaaaag
      241 cgttggaaat tccagtttgt gtttttattt cttcgcatac agaaagtgct gcggaaactt
      301 ttgaattgca aacgttagat tttatcgtaa aaccatttaa gtttcctaga tttgaacaaa
      361 ctgttaaacg aatagaagaa tttatggaga ttcgcactaa agccagtttg tttgaatcaa
      421 ctattggtgg tgatgtggtt tttgtgaaaa caggtcatga acaaacaaag gtgaaacttc
      481 atgaaatagt atatttggag gctttaaaaa actatacaat tttggttact caaacacaac
      541 ggcattgtgt tttatctaat cttggtgaat tattgcagga tgaaaatttt cagtcgtttg
      601 tgagagtgca tcgtagtttt gcggttcaaa aacactttgt tgataaaatt ggaaatcatg
      661 aaattgagct tagaaataaa gttttgattc cgattggaag aagttataag tataatgtaa
      721 aaaccattat atgagaaagt tgcttttgtt ttttttgttg tttggtcatt ttttggtagc
      781 tcaaattccg aattctttag attctttgaa agtttattta gacacaaagc ctaaagacac
      841 gagttatgtt cttgctttaa atagctatgc ttttttaaat gttcaagaag gaaagtatga
      901 agaagcggca cgcatcattc aagaaatgga gcgattatct aaaaaacttg actttggaac
      961 tggtttttac aaatcgatga acatgaaagg tgttatagca tattccaaac aaaatcctga
     1021 aaaggcgatg gaatatttta tacaatgcaa tacaatcatt aaaaaataca aacttcctaa
     1081 aaagatttat caaaattcac tcaataatat tggtatcata tacaatcaat taggggatag
     1141 agagaaggca acgaaatatg cattggagtt aattagtttt caagaaaaaa ataagcttag
     1201 tccgctaaaa tcttccccat atactcaagt tggtgataat ttaaaatttt ataaaaaatt
     1261 cgatgaagca ttactttatt ttcaaaaagc attaattgta gaaataaatt taaaagatga
     1321 ttcaggtatt gcgattaatg aaaatcgcat tggaacggtt tatgaagatt tgaagaaaaa
     1381 tcaaaaagca attgaccatt ttaaaaaagg attaacccat gctgaaagag caaattacaa
     1441 attgcttcaa gtggacttac tgaccaattt aggcagaatg tttcaaaaag aaaagaattt
     1501 tataactgct gaaaatatgc ttaaaaaagc ggagaagtat agtattgatt tagaagctac
     1561 gcaaaataca aaagttatct attcaaattt aggcgattta tattttgagc aaaaaaatat
     1621 cgctttagcc gaagattatt atattaaagc gctaaagctt tcaaaagaaa ttaatgacca
     1681 aagttttttg ttttctgcca atgagaatct tgctactttg tatgaaaaaa aaggtgattt
     1741 taggaaggct tacttctata aaactgaagc caacgttgct agagattcca tttttaaatt
     1801 gaagacttta gaaaacacag aagatttgtt gaggaaatat caaaccgaaa aaaaagaaca
     1861 agaaattgct ttaaaaaacg tccagatttc aaatgctaaa aaacaaaaat ggtattttat
     1921 tagtggtctt tttctccttg ctatcattgg aagtttgctt ttttatcaaa gtagaaaccg
     1981 taaaaaaacc aataaaaaac tacaactttt aaatgacgaa cttgaccaag ccaacaaagc
     2041 caaaacacga ttttttagta ttttaaatca cgatttacgt gcgccagtag ccaacttgat
     2101 taatttttta caattgcaaa aagaaagtcc agaattgctt gatgtagaaa gcactaagag
     2161 aatgcaagac aagacaatga gtggagcaga aaaccttttg aactctatgg aagatatttt
     2221 gcaatggagc aaaagtcaga tggaaaactt caaacctgaa ctcaaacaaa ttgcggtttc
     2281 ttcgttgttt tctgatatcc aaaaccattt ttcaagtttt gaaaatattg aaatatgttt
     2341 tgaaaacgaa gaaaacatca ctttaaaaac agacgaacat tatctaaaaa caatactgag
     2401 aaatttaact ggaaatgcaa taaaggcatt tggtagttcg actacgctca gtaaacctgt
     2461 gattctctgg aaagcttggc agcacaataa tcaaactttc ttatccatta ccgacaatgg
     2521 aaaaggcgct gatttagaac aattcaaagc actctatgac gacaaagaag tggttggaat
     2581 caaatcgggt ttaggtttac atttaattcg tgatatggca aaagttattg attgtgagat
     2641 ttttgtagaa agtaagaaag atgttggaag tacttttacg ctttttttta gggcataaaa
     2701 aaaggcctaa cgaaagttag accttaatct ctttttcact ataatgttat aacaatgcgt
     2761 caatagcatc tgtatatgtt tttttaggtg atacgcctac ttgacgacca actacttctc
     2821 cgttttggaa aattaaaacc gtaggaatgt ttctaacacc atattttgct gcaaactctt
     2881 ggttagcatc tacatcaact ttgcccacta ctgcttttcc ttcatattcc gtagaaactt
     2941 cgtcaattac tggtcctacc attctacatg gtccacacca agctgcccaa aaatctacta
     3001 ctactggttt gtctgatttc aaaactactt catcaaaagt agcatctgtt attgttaaag
     3061 ccatattctt ttttttaaat tattcttaat agtacaaaga tacgtaaaga ttattaccta
     3121 aaaatgtaac ttagattact tttaactatt tttaaattgg tttaattgat tagtttaatt
     3181 tgaaattaag ctgtaatttt tctaatcccg aaagtaattc atgagaaatt ttgattttta
     3241 attttcgact aggcatcgta attttattga cggtcaccgt tttgatatct tccggaattt
     3301 ctatttcatt ttcttcagaa aactccattt cttctgtgtt attgtcaatg tcgattgatg
     3361 tattagtcaa agtatcaatt ggcttagctt caattttgat aatttctcgt tctacaactt
     3421 caaatgttac taaattatca cctttgttgc tttgaaataa atcattcaat cgatgaatca
     3481 attcgctatg taattctttg atgtccattg ctatgattag ttttttagca aattgaggca
     3541 atacatcttg aagatattgc agtgtttgaa attgtattct tggttcacca cgttttcctt
     3601 cttgattaat ccaaccttcg cgaattaaaa ctttcataaa ggtgaaattg ttttgaatta
     3661 aaaaatgacg aaattttaaa tattcttcac caaaaatctt aaactcaaaa ttttcatcat
     3721 acccttccaa agtaaaactt ccccagcctt ttccattttt agatgttcga tgctgaacgt
     3781 tggtaataat tcctccaaag gttaaacttt tattgacgaa ttggtctaaa ttcttcaggc
     3841 tttctaattt tgaattacag aaatatttca tttcaaatcg gaaatcatct agcggatgac
     3901 cagaaatata gatgccaacg acttcttttt ctttggctag tttttccata gtgctccatt
     3961 cttcacatgg tggaacaatt ggttcaggaa tttgaatatc actgctttcg ccaaataaac
     4021 ttacttgtga tgaattttcg ttttcttgat atttggaacc ataacgagtt gctttttcta
     4081 agaaagtaat accatcgcca tcatcatgaa aatattgtgc ccgatgcgtt tctgaaaatc
     4141 catcaaaacc accagccaaa gctaaacttt cgaaagcttt tttattggca gcacgcaagt
     4201 ctattttttt ggctaaatca aaaatagatt tgtactttgc tttttttctg ttttcaacaa
     4261 ttgtatccac tgctggtgcg ccaactcctt taatagctcc catgccaaaa cgaactgcat
     4321 agttttcatt aaccgtaaat ttatagaaac tttcattcac atctggacca agaacattta
     4381 atcccattcg cttgcattct tccataaaaa aggaaacttg tttaatgtcg ttcatgttat
     4441 tagataaaac cgcagccata tattcggcag gataatgtgc ttttaaataa gcagtttgat
     4501 agcctatcca ggcgtagcaa gtagagtgcg atttgttaaa agcatattgc gcaaaagctt
     4561 cccaatcttt ccaaattttc tctaatttgt cctctgcatg accattttcc atagcttgag
     4621 agatgaattt ggatttcatt ttgtccaaaa cctctttttg ctttttaccc attgctttac
     4681 gcaaaacgtc ggcatcacct tttgagaaac tggctaattt ttgagacaaa agcattactt
     4741 gttcttgata aacagtaata ccataggttt cttttaaata ttcttcgttt gcttcaagat
     4801 cataaacaat ttcttcttct ccattttttc ttttaacgaa tgatggtata tattctagtg
     4861 gtcctggacg atataaagca ttcatagcaa ttaaatcacc aaaaacagtt ggttttaaat
     4921 cgcgcaaata tttttgcatt ccaacacttt catattgaaa aataccaacg gtttcaccac
     4981 gttgaaaaag ttcatatgtt tttacatcat caataggaaa ttcatcggga ttaagctcga
     5041 ttcctgaccg gtattttaca agttttacag tgtcttttat taatgtcaat gtttttaaac
     5101 ccaagaaatc cattttcaac aaaccagcac tttccactac ggagttgtca aattgagtca
     5161 catatagagt ggaatctttt gcagtagcta ccggtacaaa gttagtaatg tcgcttggtg
     5221 tgataatcac tccacaagca tgaattccgg tatttcgcag atttccttcc aatattttag
     5281 cttgattaat cgtttcgctt cctaaatcat ctgcattggc taattcgatt aattctttta
     5341 ctttgtcgta ttcctctgct cgcaaagcac tttttaatgc tttatcgtct aaagaaaata
     5401 ttttatcgag ttttaaattt ggaaccaatt tggcaatttt atcagcttca aaaagaggta
     5461 aatctaaaac tctggcagta tcgcgaatgg atgattttgc tgccattgtg ccataagtga
     5521 taatctgtgc cacttgattg gaaccgtatt tgttgatgac ataatccatg actttgcttc
     5581 gtccttcatc atcaaaatca atatcaatat cgggcatgga aaccctatct ggattaagaa
     5641 aacgctcaaa aagcaaatcg tattttaagg gatcaatatt tgtaatccaa agacagtatg
     5701 ctactactga gccagcagca gagccacgac ctgggccaac cgaaactcct aaatttcggg
     5761 cttcggcaat aaaatcttgt acaattaaaa agtatccagg ataacctgtg ttctcaatgg
     5821 tttttagttc aaagtctaat cgttcttgaa tttcaggagt aatttctttg tatctttttt
     5881 ttgctccttc aaaggttaga tgtttcaaat aggcattttc acctcgtttt cctccgtcaa
     5941 gattgtcatt ttctactaaa aattcggttg gaatatcaaa ttttggcaat aaaacttcac
     6001 gagccaaatc ataaatttca attttatcaa ctacttcttg aatatttgaa atggcttctg
     6061 gataatcttg aaacaattgt ttcatttcat cacccgactt aaaataatat tcttgattgg
     6121 gcaacccata tctaaaacca cgtccgcgac caattggtgt tgattgtttt tcgccatcgc
     6181 gcacacaaag caaaatatcg tgagcgttgg catcttcctt gtttacataa aaaacattat
     6241 ttgtagcaac tgtttttacg ttatgtttat ttgccaaatc aatcagagca ctatttacac
     6301 gattttcatc ttcttgatta tggcgtgata attcgatgta aaaatcctct tgaaaatttt
     6361 ctttccacca aagtaatgct tcttcggctt gattttctcc gatatttaaa agtttatttg
     6421 gaacttcacc atacaaactt ccagaaagta ctataatgtc ttctttgtat tgttgaatga
     6481 tgtttttatc aattctggga acataataaa atccttgtgt aaaggcaatt gaggacattt
     6541 ttgccaaatt atgatagccc tttttatttt ttgctaataa aacgatttga taaccattat
     6601 cctttctaga cttatctaag tgattttcac acacaaaaaa ctcacatcca actattggtt
     6661 ttatgatttt ttcagttggc tcttcaccgt tttcaacagc agttttgttt tttgtttcta
     6721 cagatttatt atgatacaaa atatcacgaa caaagtgaaa tgctcccatc atattaccaa
     6781 tatcagttaa agcgacagcg ggcattttat tttttgctgc cgtttttacc aaatcttgaa
     6841 cactaaccgt ggattgtaaa actgaaaatt gtgaatgatt gtgcaaatga acaaaatcaa
     6901 catcaattaa ttgttcttga ttttgcttga tagtttcttt ggaaatagga acatttcctt
     6961 tttcgccaaa ttgttgacgg attttttcag aagcttcttt aagattgatg tgttttaatc
     7021 caataagttg aatgggtttt ggatttttct cattaaattc tctgaaataa tccgctggaa
     7081 catcaagttc ttcttttgta aaaatttctc tttttattaa ttcaaggaaa caacgtgttg
     7141 tagcttctac atcggcagtt gcattatgcg cttcattaaa tttttgatta aatagatatt
     7201 cgtgtaattc agttaaagtt ggtaatttga attttccgcc tcttccgcca ggtaatttta
     7261 acaactcagc ggttacttcg gtacaagtgt ctaaaacagg tttttcgttc attggagaat
     7321 caatccccat tcgataaaat tcacacccca ttatattaat atcaaaacct agattttgac
     7381 ccactataaa tttggatttg cctaatgcga tgttgaattt atccaatact tctttgagtg
     7441 aaacaccttg ttcttgggct aattctgtag agattccgtg tattttttcg gcatcaaaag
     7501 gaatattgaa accttcagga ttaattaaat aatcctgatg ctcgatgagg tttcccattt
     7561 catcgtgcag ttgccatgca atttgaacac atcttggcca gttatcagtg tctgaaatag
     7621 gagcagacca attacgaggt aaaccagtag tttcggtgtc aaatattaaa tacatagaag
     7681 ggtgaaattc caaaaattaa attccaaatt ccaataaaaa ctatattttt agtgtgaaaa
     7741 aggagaactt caaaattaca tttttggttt atagaaatca attttagtta tcaacaaaaa
     7801 caaaaaaacc atctcaatga gatggttcta ttttttataa aaaagagtaa ttaatatctt
     7861 tctctatttc caccaccata gtttccacca ccacggttgt ttctgtcaaa acctcctgaa
     7921 cggttgttgt tgaaagattt tctttcacct tcaggttttg gctcagattt gtttactaca
     7981 atagtacgtc cttcaacagt agcaccattt aattcttcaa ttgcttttaa tgcttcgtcg
     8041 tcgtttgaca tttcaacaaa accaaatcct ttacttcttc cagtaaattt atcagtaata
     8101 actttaacag actctactgc tccgtaagcc tcaaaagact ctcttaaatc tgcttcctca
     8161 atactccaag gaagacttcc aacaaaaatg ttcatatata atttttaaaa ttcaatacaa
     8221 aggtaagtta aaaaaccata ctaaatactt attttacatt tatattccta gataaaaatg
     8281 aaaaaaccat caattgatgg tttttataca ctatttattg ccagtacttt atatagaagg
     8341 ataaatttga acattataaa actcgcctga ttggaaattg acaacatcag cactaagtgg
     8401 attgttgttg gaattaacag cattgaactt aaattttcct gaaatggtca ttttaacatt
     8461 gtcaaattgt gtaatttcaa tttcaccatt actgttttga acatttacaa ctctaaattt
     8521 actgttcaaa tttccacctg taattgtaac gatgtcacct gccatgtatc catttcctct
     8581 agataataag gttacttctg ttacgcctcc gtttgcattt gctttgatgt tgacactcaa
     8641 accacttcct gaaccaccag tagtggcaac cgaagttgca tcagtatatc ctgtgcccac
     8701 attagtgagt gatacggttg aaacaggtcc tggagtagga atcgttgcat attctatcgc
     8761 tacatcatcg aatgttgtag catagctggc aaaattactc gaatttgtag atcctaattt
     8821 gtattttcct aaattagtgc ttgaagtgcc taatgttaaa acttcatact gagcataggc
     8881 ttcaatggtt agtttgccat cagtaacata ggctcgaaca tcagctgctc tccaaaaaga
     8941 gtcgtttttt agaccttgta atccaggatc attgaatttt acgtcttcag aacaagacat
     9001 cattaaaaaa gtaatcgcaa ttagggacaa aaattttctc atcattttaa tttttacttt
     9061 acaaaaataa ctttttaata gaattagcat aaacttattt gaaaaaataa cagtttttgt
     9121 atgaatttta gaaaaagaaa ttaattagta gttgaaattg ttgcgattat atgaaaaaaa
     9181 actatctttg cgcccttaaa taaccgaggt cgagtacctc aaattaatca aaatagatta
     9241 tgtcagtaaa aattagatta caaagacacg gaaaaaaagg gaaacctttt tactggatcg
     9301 tagcagcaga tgcacgttca aaaagagatg gtaaattctt agaaaaacta ggaacttata
     9361 atccaaacac aaacccagca acaattgatt tgaatgttga tggtgcagtt caatggttac
     9421 acaatggtgc tcaacctacg gatactgcaa gagcaatact ttcttataaa ggtgctttaa
     9481 tgaaacatca cttagacgga ggtgttcgta aaggtgcttt aacacaagag caggcagatg
     9541 caaaattaac ccaatggtta gaggcaaaat ctggaaaagt tgactcaaag aaagatggtt
     9601 tgtcaaaagc taaagatgaa gcgaaagcaa aagctttaaa agccgaaaaa gaagctaatg
     9661 agaaacgtgc tgcaaaagca gtagaagttg cagaagaaac tacagaagaa gttgttgcag
     9721 aagaagcgat ttcggttgaa gatgctccag tagcagaagt tgttgcggaa gaagctccag
     9781 ctgttgagga aactccagtt gcagaagaaa acaacgaaga aaaagaagca taattttttt
     9841 gagcaattat gcataaaaaa gagtgtttct atttaggtaa aattgcaaaa aaatttagtt
     9901 tcaaaggcga agtcttattg tatttagata ctgacgagcc agagttatat gaaaatttgg
     9961 aatcggttct tgttgaaatg aacaagaatt tgattccatt ttttattgaa aatagttcac
    10021 tacacaaaaa tgactttcta agagttaaat ttgaagaagt agattcagaa gaagaagccg
    10081 attccatttt gggttgtgaa atatatttgc ctcttaccat gttgccaaaa cttgaaggta
    10141 acaaatttta ttttcacgaa gttataggtt ttaaaattga agacaaacga cttggaattg
    10201 ttggtgaaat tgtttctatt aatgacacaa ctgcacaacc tctttttgaa gttttaaaca
    10261 atggagttga gattttaata ccaatgattg accacttttt acttgaaatt gatagaaaaa
    10321 acaaaaaagt ggtaatggat ttgccagaag gtttgatcga aatgtacttg taaaaaaatt
    10381 ccaaaatttt aaattccaaa atccaaagtt caaaagtaat tctgatattt taaattttaa
    10441 agtttatgaa taaaccattt gatttagagg aaagaacttt tttgtttgct aaagaatgta
    10501 ggatttatgt tcaaaaactt ccaaaaacaa tttcaaacct tgaagatggt aaacaattgg
    10561 ttcgttcctc tggttcggtt ggtgccaatt atattgaagc taatgaaaaa ttaggtgata
    10621 aggatttttt attcagagtt aaaatttaaa gaaaagaagc taaagaatca aaatattggc
    10681 taggattatt acaagaatta aatcctgaat taaatgaagt ttctagttta ttaataattg
    10741 aatctgaaaa gttaagaaag atattatcat caattattac gaaaagttcc taattcgtta
    10801 ttcaacctaa ttggaatttg gaatttaaaa tattggaatt taatcactat gttttcattt
    10861 aaaaaattta ccgttctgca agataaatgc gctatgaaag taggcacaga tagtgttttg
    10921 cttggtgctt ggtgtccaat tgacaatcat cctttctctg ttttagatat tggtgctgga
    10981 accggaattt tatctttgat gttggcacaa agaagtaatg cggaacaaat tgatgctatt
    11041 gaaattgatg aaaacgcata cgaacaatgt gtggaaaatt tcgagaattc accttggagt
    11101 gatagattgt tttgttttca tgcaggatta gatgaatttg ttgccgaacc tgaagaagaa
    11161 tatgacatta ttatttcaaa tccacctttt tattcggaag atttcaaaac tgataattct
    11221 caaagagatt tagctagatt ccaagatgct ttgccatttg aagaattagt agaagctgcc
    11281 aatttattat tgtctgaaaa cggcattttt gctgtgatta ttccttttaa agaagaagaa
    11341 cgatttattg atttgtgtgc cgaagtggaa ctttttcctg tgaaagttac tcgtgtaaaa
    11401 ggaacacata acactctagt tgttagaagc ttattggctt ttaaacgcta tgaactttct
    11461 gttttagaag ctgatgaatt ggttatcgaa atcaatcgtc atgaatatac ggatgattat
    11521 atcaatctta caaaagagtt ttatttgaag atgtaatact agcccagatg ggaacggcat
    11581 ccttttttaa tttttcataa aattaaaaaa gatatagtgt acagctggaa aaagctccta
    11641 aaaattataa cacttgttaa actgaaaatt aattaagatt atgtagcttt gcaaacgttt
    11701 tcgtgattac aattttacat aatacaacct atattttatg aaaccagatt tatttcaatc
    11761 gccagattat tatttacttg acgatttatt aactgaagaa cacaaactgg ttcgagattc
    11821 tgctcgtgct tgggtaaaaa aagaagtttc gccaattatt gaagattatg cgcagcgtgc
    11881 agaatttccg aaacaaatca tcaaaggctt gggcgaaatt ggcggctttg gtccttacat
    11941 tcctgaagaa tatggcggtg caggtttaga ccaaatttca tatggtttga ttatgcaaga
    12001 gattgaacgt ggtgattctg gtgttcgttc tacttcgtct gttcaatcat ctttagtgat
    12061 gtatcctatt tggaaatttg gaaccgaaga acaacgcatg aaatacttac caaaattggc
    12121 tacaggtgaa tttatgggtt gttttggatt aactgaaccc gatcatggtt caaatccaag
    12181 tggaatgact acaaatttta aagacatggg cgatcattat ctgttgaatg gtgccaaaat
    12241 gtggatttcg aatgcgccat ttgccgatgt tgcagtagtt tgggcaaaaa atgaggaagg
    12301 aagaattcac ggattgattg tagaacgcgg tatggaagga tttactactc cggaaacgca
    12361 taacaaatgg tcgcttcgtg caagtgcaac cggcgaattg atttttgaca atgtgaaagt
    12421 tccaaaagaa aacttattgc caggaaaatc tggacttggt gcaccaatga tgtgtttgga
    12481 ttcagctcga tatggaattg cttggggtgc aattggtgcc gctatggatt gttatgatac
    12541 ggctttacgt tatgctaaag aacgaattca gtttgacaaa ccaattgctg gaactcaatt
    12601 acaacaaaaa aaattagccg aaatgattac cgaaattacc aaagcgcaac tgttaacttg
    12661 gcgtttagga gttttaagaa acgaaggaaa agccacaacg gctcaaattt cgatggcaaa
    12721 aagaaacaat gtagatatgg cgataaatat tgctcgcgaa gcgcgtcaaa tcttgggtgg
    12781 aatgggaatt acaggtgaat attctatcat gcgacacagt atgaatttag aatcggtaat
    12841 tacgtatgaa ggaacgcatg atattcactt attaattact ggtgctgaca ttacaggaat
    12901 tccagctttt aagtaaattt ccaaatttta aaatgtcaca ctgagcgcag tcgaagtgac
    12961 acttctaaaa tggagtaaat taaaaattta taaaatgctt ctcattcacg ggaagcattt
    13021 tgcttttaca gttagtttta acaaccaaaa tggatttaaa ttcgtatata aataatacac
    13081 aaaacactat tatataaatg ggaaaaacta aatttatttt attaattctc tgtttgatta
    13141 ttgtaagttg tagtagcgac aataaagtta ttagcaactc tgcaacaatg aaaaatataa
    13201 attatttggc acttggcgat agttacacaa ttggccaaag cgtttgtgaa aaatgtcgct
    13261 ttccaattca attacaagat agcattaaga agtacattga ttctaacgac actttttcaa
    13321 ctcaaatcat tgctcaaact ggttggacga caactaattt aaaaaatgca attgctactg
    13381 caaatccatc ctcaaattat gacttcgtta cactattaat tggtgttaac aatcaatatc
    13441 aaaacaaacc gtttagcata tacgaacaag aatttccaga attggttaat caggcaatta
    13501 cttttgcaaa aggaaataaa aataatgtaa ttataatttc tattccagat tatgctttta
    13561 ctccttttgg gaatggctca atgacaattt caaatgaaat agatttgtat aattcgtttg
    13621 ccgaaaatta ttgtaatgaa aataatatta cctttgttac tattacagac attactagat
    13681 taggattgtc gcaaccaaac ttagttgcta cagacggttt gcatccttca gaacttgctt
    13741 attcaaaatt tgtagagcga attttgccaa ttgcaaaaat gaaactaggt ttgtaattat
    13801 tgctcaggag caatttcaat ttctaaacca tctaaatctt cagtaatagg aatttgacaa
    13861 ccaagacgag aattgtcttt gacatgatag gcttcccaaa gcatggcttc ttcatcagaa
    13921 ttgcgttcca aagaaatttt atcgtttaaa acgtaacatt gacacgaagc acacattgcc
    13981 attccgccac aaactccaac tgttccttct tcggccaact catacatacg gattaattcc
    14041 atgatgttta aattcatatc cgttggtgca agaacttcgt gaagttgtcc gtttctgtca
    14101 gtgattttta gggttatgtc ttgtggcatc tattctatcg cttttacaac cgctttttcg
    14161 gcttcttttc gagttccatc aaatccgtca atgccagaaa cggtcgtata tttcaataca
    14221 tatttcttgc caggattaat tctttgatat acgctttgac acatcaatgt tgcttcgtga
    14281 aaaccacaaa gaattaattt taattttccg ggataaatat tcacatcgcc aatggcataa
    14341 attccatcaa tattggtttg ataatctaat gcgttgttta ctttgattgc atttttttca
    14401 atttctaaac cccaattggc aatcgcaccc aattttggtg ttaatccaaa aagaggaata
    14461 aaataatcag tttcaacatt catccttgca ccattaactt ctatgtcaac cgattctatt
    14521 ctttcggagc ctttaaatcc aataacttca gcaggtgtga ctagtttaat ttttccttcg
    14581 cttttcaatt cttgaacttt gtcaacagaa tctaaagcac cacgaaattc atttcttcga
    14641 tgcactaaag taacttcgga agcaacatta gaaaggaaaa tactccaatc taatgctgaa
    14701 tcgccaccac cagaaataac aaccttttta tttctatatt tttcgggatc ttttataaaa
    14761 taatcaacac cacgatcttc tttttcatag aattctaaat ctttaagaat tggttttctt
    14821 ggttcaaatg ttcctaaacc tccagcaata gctatagctt ttgctttatg ttgcgttcca
    14881 gcattagtgg ttacaacaaa agttccatct tctaatttag taatggtttc agcagtttca
    14941 ccaagtgtaa atccaggttg aaattgtttg atttgttcca tcaaattatc aactaaatca
    15001 ccggctaaaa cttctgggta accgggaatg tcaaaaattg gttttttggg atataattca
    15061 gccaattgtc cgccaggttg tggcaaagca tcaataatat ggcatttaag ttttaataat
    15121 ccagcttcaa aaacagcaaa gagtcctgtt ggtccagctc cgattattag tatatcggtt
    15181 tctatcattt ttagtcgtaa caaggtttaa agccacaaag atgcaacgtt tctttatcgt
    15241 tacaaatgac ttttatcata tggccaatta aacaatattc tctacagttt caatttgtgg
    15301 agcgtatttt tttatagtag tttctacacc agctttcata gtactaatac tcaaactgca
    15361 agaagtacat gcaccttgaa gtcgcacttt cacatgttta tcttcatcta tagaaaccaa
    15421 ttcgatgtcg ccaccatcag aatttaaaaa aggcctgatt tcgtctaatg ctttttcaat
    15481 atttaaagtc aattcttctg ttgtcatctt attttccttt tacagcagaa catcctgcca
    15541 ttgtggttat tttgattgct tcagttggag ctaaatcttc atttcttctt actgtttctt
    15601 ccacaacatt tcttgcaata tcttcaaata ctttttctaa tgatgaagcg gtttgtaatg
    15661 cggctggtcg accataatct ccagcttcgc gaatactttg tactaatgga acttcgccaa
    15721 gaaaacgaac gttcaaatct tctgcaagat tttttgcgcc ttcttttcca aaaatataat
    15781 atttgttgtt tggcaattct tctggagtga aataagccat gttttcaata attcctaaaa
    15841 ctggaacatt tatagcatca ctcatgaaca ttgaaactcc ttttttagcg tcggctaatg
    15901 ctactgcttg tggagtagaa actacaactg ctcctgtaat tggcaacgat tgcattattg
    15961 ataaatgaat atctcctgtt cctggtggta aatcaattaa catgaaatct aattcgcccc
    16021 aagctgcatc aaaaatcatt tggtttaacg cttttgaagc cattggtcca cgccaaatta
    16081 cggcttgact tggtgaagta aaaaatccta ttgaaagtaa tttgatttca taactttcga
    16141 ttggtttcat ttttgatttt ccatcaactt caattgaaat tggcttttca ttttcaacat
    16201 caaacatgat aggcatactt ggaccgtaaa tatcagcatc taaaacacct actttaaagc
    16261 ccatttttgc taaagtaacg gcaagatttg cagtaactgt tgattttcca acgccgcctt
    16321 ttcctgaagc aacagctatt atattagaaa ttcctggaat tgcttttcct ttaatttcat
    16381 ttggattttc ttttgcctcg actttaatat tgactttcac aactgcatca ggtgaaaatt
    16441 tttcgtgaat taattttttg atatcgtctt cagctcgttt ttttatgtgc attgcaggag
    16501 ttgaaagtaa taaatcaacg actacttcat ctccaaaagt gattacattt tgaactgcgc
    16561 cactttcgac catatttttt ccttcaccgg caattgtaat ggtttctaat gcttttagta
    16621 tttcttttct atctagtttc atttgtttaa aagtttcaag tttaggtaat aaagttcttt
    16681 aacatttatt taaactgaat atgaattgca aagatataaa gaagtttaat ggtttaaaag
    16741 tttaagagtt taaagttttg atggacaaat gataaagttt tgttataaag tgttagtttt
    16801 aatcaaattc attttaaacg caatgtattg aagttgtaat ttgtttttta taacctcata
    16861 ttgagaagtt aaccagttgt ttttggccat agagtaggag aaaacatcta tcactcctgt
    16921 agtgaatttt gcttgagttg tagtaaacga tgctctggca aaattttcta tttctattaa
    16981 cttagcttgt atactttcta aatttttctt gtttttttgt tctagttcaa gttgttgata
    17041 tagactattt ttttcttgtt cgatttttaa ttttgatttt tcagagtcaa tttttgcagc
    17101 agtaattgct cggttgtttt taaaaccatt aaaaacagga atagaaattt gtacgccaac
    17161 ctgttggttt ttgttatttc ctatttgatt gctaaaattt tcaactacta tttcaggttg
    17221 atttaaaggt ttataataaa atgatgctaa ttgataaaat gttgaaaatg ttggtaaatt
    17281 attggctcgc tgaaagttga tttctttttc gcttttttca agagctaaaa gtgctgcttt
    17341 aatttttggg tttttcaaat tctctaaatc attttctggg tttgcaatgg tatgttctaa
    17401 aaccaccgtt tctatgttta aatccttata attaatcaat tgaaatagtt gtgttttttg
    17461 aattgttaat aattgttgtg tttctaaaaa acgtttttct tcttgtgcat aattaaattg
    17521 aatgtcatac aaatcacttt ttggtttgct accaatggta acttctttgg atattctatc
    17581 taaattatac ttactgtttt gtagctgtat ttcttgaatt ttttgcaatt cttgagtaaa
    17641 aagagtttga taaaaacttt caagtacttg tagtttatat tcgttctcaa taatttcttt
    17701 ttcagccata gcaatttcga ttgttatttt atctttttgt gcttcggcaa ttttgctaaa
    17761 atccaacaaa ttcatttggg cacttaaata aaaattgtcg tattgtatat ttgaacttac
    17821 tctaccattt gtagaaggat cgatagttga gccaaaatta taactttgat ttccatataa
    17881 attaacttga ggcaacaatt cgtttagcat agaaactttt gtcttttggt ttcgtttgat
    17941 ttctaattgt tgtattttta tggctaaatt gttttcaatt cccagcgcta tacatttttg
    18001 taacgtccaa ttagtttctt gagcaaaaca aaagcttgaa aataaacata aagaaattaa
    18061 aaagcttgcc ttattcatat ttcaaatatt ttaaaacatc aactttagtg gcttgatatg
    18121 ctcttgaaag tacaactatt aacgttaaaa acagtaatgc gaaaaaccca acgataaacg
    18181 gtagaacaga aatatctatt ctaaaaacaa agttttctaa ccatttatta agaaggaaat
    18241 aaactggaaa taaagctgtt ataaatccaa gtatgcaaaa aactatatat tgtttggata
    18301 attcttttag caaaacatta gtttctgctc cgagcgtttt tctaatggcg atttctttca
    18361 ttcttcgctg gattgaataa cttgccaaag caaacaatcc gaaaagtgca attagaatta
    18421 caataaaatt taacagtgaa aacaagtttt tttgtttgac ataactggca taggttctag
    18481 catattgttt atctacaaaa tcatatgaaa atggatatac ggtatcaaca tttttagtcc
    18541 aaaatttttc aatctctgca atagtttgag atggattttg agcatcaatt ttcacataaa
    18601 ttctacttgc attttgcaac atccaatcta ctgttttata gtgaaaaaac accattggcg
    18661 gaatgttttc ttgtggtcca aaaagatgaa aatctttcac aactccaata atttttagct
    18721 ttttatcgtt ccaatttact tctttaccaa taggattttt ttcattcatc attcgtaaag
    18781 cagtttcatt gactaacatt gaatttatgg tgtcagaagc tattttgttt gacaaaaacc
    18841 taccttcatt taattgaatt tgcatcattt cgagcattcc aaaatctaca cccatatttc
    18901 gagcttgaat gttttcactt cctctatatg aaaatccaga agttgaacca ttgccagagc
    18961 caaattcaaa agctcctgta ctcacttgtt ttactccttt tatttttgaa agttcatttt
    19021 ttattaagag ataacgactt tctacttttt ctttataatc cttttccctc caattgtagc
    19081 cattccaata ccaaactgcg gcaacttgat cgcctttaaa ccctaaattt tttgtagaaa
    19141 catattgtat ttgttgataa acaatataag aaccaatgat aaaaaaggac gcaatagcga
    19201 attgaacaat cagcattcca ttacgcaacc aaattccgtt tttacttctt ccaaaattac
    19261 ctttcaacac ctttaatgtt tcaaaatttg acacatatat agctggaaaa atacctgcaa
    19321 aaatgatagt aataataaaa attacaacaa tttgcatata aaattgattg ccaaaaatga
    19381 ctaatttttt acctaaaaaa tcattgtagt aaggtaagga aagctcaacg ataactaaag
    19441 ataaaagtat tgaaaataaa acggtaatgt tggtttcaaa caggaattgc ttaatgatat
    19501 tactttttga cgctcctaaa attttgcgaa caccaacttc ttttgctctt tttacagcat
    19561 tagcagtggc tagatttaca taattaacga tagataacac taaaattaaa attgataatc
    19621 caatcataat cagaagaaat tgatagtttc cttttccttc tgcaaaacat tcagttattg
    19681 catgtaatcg aatttcatct aatgattgta aataaaactt agtggatgtg atgccatttt
    19741 ttttaagcca ttcttctggt gatataccct cttgtttagc attttttaca gtactgtttt
    19801 catgcatcaa gcgttccatt cttgcaatca ctttatcttt atcatcagga ttttttaatt
    19861 tcaataataa tccataactg tagtttcccc aatggtcttt attttcaatc attctttcat
    19921 cgattgagtt tacaattact tcgggtgcca tggatgattt tcctggaatg gtataaacac
    19981 atcctacaac taactttcta gaaccatcat ctaaagtttt acctaatgga ttttcgtttc
    20041 caaaaagttt tctagccgca tcttgtgata gtgcaatatt ggtattatca tttaatacat
    20101 ttttagtact tccaccaaca attgtaaacg gaaacatatc aaaaaatgag ttctgtgcat
    20161 aggtaaactt tactatttct tttttgtttt tatacattaa aacagcttct tcataccaat
    20221 tatcaaagta gcaatagctt tctaattcag ggaaatcttt tttgaaatat tcttcaaatg
    20281 gtactacgtt tgatgcccaa tactcatcta tcccgatttt tgaaatactt ataaaaacat
    20341 tatctttccc tggattccaa ttgttgtagc tttgttcatc attccaatac aaagtggcaa
    20401 aaattaaccc tgcaattccg atgcttaatc ctaaaacatt taaaaaagta aacagtttgt
    20461 tgttcttgat atgaaagaga aaaaggttgg tccaattttt taacatgatt gttcgttttt
    20521 tgattgatga ttatttttga attacagaac ctccagagct tttttctttt gagatgtttg
    20581 atggattgcc ataaaatgaa atacttccac tagatgaagc tttagcgttg attgaattgg
    20641 acgcataaac cgaaattgat gccgaactgc tggctgttgc agttgcgttt tctgttttta
    20701 aatcttttaa attacaatcg ccagaactac tagcttttac cacaacattt ttggcttttc
    20761 cttctattga aactgtagat gagctacttg tctctatagc tatttctttt gccataattt
    20821 cacctgtaaa tgtgccacta gatgatgtgt ccaaagtcac gacatcactt tcaaagtttc
    20881 ctttaatggt tgccgatgaa cttaccgtta gtaataattt ttttgactca ttaacattca
    20941 aaatttttac aacagatgaa gaactggctt ttatttcgtc taaattaggt cctgaaatat
    21001 taatttttag ggttttaaag cttaatttat ttttccagtt tttatttttg cctttttgat
    21061 tgtaactgtc gttttctatg tatattttaa gtgtttcacc tactgtttct gttttaatga
    21121 attgcagatt tttttcgtca tctgtttcta tttttaagct tttagtggat gatatcgtat
    21181 agtgtacatc aatagaatta gacactttca attttgaaaa atctgctact tctcgatttt
    21241 cgactacttg tccaaaagaa aaaagactaa taataatagt gctaagggtt aaaattattt
    21301 tactcatggt tttatagttt taagaaatta atacatcaac atttttactg ttattttttt
    21361 ctgaaagtat cattccgtct ttcatgaaaa tggttttttg tgaaaaagaa gcatcataat
    21421 ccgaatgggt taccattaaa attgttgcgc cttgtgcatg caaatcggtt agtaattcca
    21481 tcacttcatt tccgttttta ctgtctaaat ttcctgttgg ttcatcagca agaattattt
    21541 ttggatcatt aattaatgct cgagccacgg caacacgctg ctgttgtccg ccagaaagtt
    21601 gttgaggata atgttttaat cggtgtgaaa ttcccagacg ttcagcaatt ttctctactt
    21661 tttgtttgcg aactgttgca gaaacatcat tgtaaatcaa aggcaattcg atattgtcaa
    21721 aaaccgaaag ttcgtctatt aaattgaaat tttgaaaaac aaaaccaata ttttgctttc
    21781 taatttttga tttttctcgc tcttttaagc caaagatttc ttgagataat aattgataac
    21841 ttcccgatga tgcgctgtct aatagtccaa ctatgtttaa aagagttgat tttccgctac
    21901 ccgatgcacc cattatggtt acaaattcgc cttgattgat tgtgattgaa acttcgtgta
    21961 atgctttggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttgaagtaa
    22081 tcactttttc gccaacttgc aatccttcca agatttcgta atataaagga ttttctcttc
    22141 ctatattaat atttctgcgt tcggctttat ttcctttaat tacaaaaatc cattttcctg
    22201 cagtttcttg gcaaaaactt cctttgctaa ttaatatgct ttccgttttt tcagacaaat
    22261 taattcgtac accaaaagac aaaccttggt taagttctaa attttcattt tttataaagt
    22321 ttaattcaac aataaagcgt ccgcttttta tttctggaat tacctttgaa ataattactt
    22381 ctactggttt ttcattaaat tctattgtgc cttttaaacc ttcggctatt ctatttagat
    22441 aaaattcatc cacttctgca actaatttat aacctttcaa aacatctatg gttccaatac
    22501 tatttccttg tgtataggtt tttcctaaaa taggttcaaa cgaagatagt cgtcccgaaa
    22561 gtggtgctgt aatcaaaaaa ttatttttat tgtttcttaa aattgacaaa cttttattca
    22621 taatagaaat ggattggtta agttgtccaa tttgtatttg attggcttgt ttttctttag
    22681 ttacactttg tttgataatg tccattcgtt ctttttgaaa gcggaaattt tcttgtgttt
    22741 tttgccattc gtttttcgcc aaaatttctt gattgaataa cttctgattc aattcatata
    22801 agttttttgc atcttgatag tcgtgttcaa tagaaattaa atctttggcc aaatttagtt
    22861 cttgatttct caaatctagt tttgctttgt tcaaattatt gatttgctca atgatagccg
    22921 tttcttgttg catataattc aattctgtat ttggattata caaacgagcc aatggttgac
    22981 cttgagaaac cctatcgcca ttatttacaa atatttcttg tactgcgccg ccttctatca
    23041 cgttgactaa cattgaattc aatggagtaa ctttggcttg aaaaacaata aaatcttcga
    23101 aaaaaccttt ttcaacggat ttgatagaaa tttcagactt attgacattt aaacttcttt
    23161 ttttagttaa tgaggttata acaaaataac ctaaaaccaa taaaataggt aaggcaagta
    23221 atccatattt ttttttgtta tttttacgct tgatgatagt gtccattact ctgattttac
    23281 ttgtatatta ggaatattgt gccaaaaatt aaatgtttct aaatccttta aattcagcct
    23341 aatataaata tcacaataaa gacactgttc aataatgaac acaatttgtc cgaaaacgaa
    23401 cagcaaatga aaaaaacaac tgctacaatt ttagttattg acgatcaaga agacatcctt
    23461 tttacggcaa aaatgctttt aaaaaaacat tttgaaacga tttatactct aaataatcca
    23521 aagaatgtaa ttcagttatt agctgaaaat caaattgatt tagttttatt agacatgaat
    23581 tatcgcattg gttttgaaga tggaaaagaa ggcttgtatt ttttaaaaga aataaaaaaa
    23641 ttagcgccaa atacagttgt aatattaatg actgctttcg gaaaggtaga aaccgctgtt
    23701 gaaggattaa aaaacggtgc ttttgattat gttttaaaac cttgggacaa cgacaaattg
    23761 ttagaaactg taaaaaaagg tgttggtttt gctcgtaaaa atcagaagga tataattcta
    23821 aacgaaagta ctaacaacta ttttattgga acatcaaaaa acattgaaaa agcctattca
    23881 ttggctgaaa aagttgcaaa aacagacgcg aatgttttga ttttaggcga aaacggaaca
    23941 ggtaaatatg taatggcgca ttacattcat caaaattcgg gtagaaaaag taacgaattt
    24001 attcatgttg acttgggttc tttaaacgaa aatatttttg aaagtgaact ttttggctat
    24061 gtaaaaggtg cttttacaga tgcaaaaatc gataccattg gaaaatttga agccgctcaa
    24121 aaaggcacta tttttttaga tgaaattggt aatgttcctt tgcatttaca atcaaaactg
    24181 ttacaagtta ttcaaacaaa aacggtaact cgactgggtg aaaccaaacc aagaaaaatt
    24241 gacgttagaa taataacggc taccaattta gatttaaaag cagaagtagc ggcaaaaaat
    24301 tttagagaag atttgtatta tcgcattaat actatggaaa ttgttttgcc tgcattacga
    24361 gaacgaatag aagataaaat tccgttagcc catttccttt tagaaaaaat gatagataaa
    24421 tatgaaaaaa ataaaatcat atttgacgct aaagctctag agcaaatagc aaaacatgct
    24481 tggaatggca atattcgtga aatggaaaac cgtatagaac gtgcagtgat tctttgtgaa
    24541 aacaacacta taaaaccttc tgatttagat ttagaaatag tattgcttca tgaacaacat
    24601 gatgattttc aactttcaga tattgaaaaa aatactattg aaaaagtttt gttaaaaaac
    24661 gaaaacaata tcagtaaatc tgccgaagaa ttaggcttgt ctcgtgcagc tttgtatcga
    24721 agattggaaa aatataacat tcaaacgtct taaaaatgtt gtctaaattt agaatttaca
    24781 atcagctttt tattagaatt ctacttgtat tacttggctt tgcggttgtt ttttatttac
    24841 taaatcaagg cttgttttat agttttgtgt ttgttagttt tattgtaatt cttacaaata
    24901 tagaattata tttttatcta aaaaatgcct ttttatttta tgacaaaaca ataaatgcta
    24961 ttttaaataa cgacttttct gctgattttt cgaaacacaa atcgtataaa aattacaacc
    25021 gattatttca attgtatcat caactgaaag agaagcaaaa cgaacaggtt tctaaagaca
    25081 tcatatatcg gtccatatta aataacattg aaacaggaat tttaattctt caaaaagaag
    25141 atgccgaatg gtcaattttt ttgatgaatg attttttttc aaaacatttt gaagtcccaa
    25201 aagtaacaaa gtggcattac ttgagagagc atttacggtc gttatgtgaa ttaatagaaa
    25261 gccaaaattt cagtgaaatt aaaacctcgc tacaaatcag ggtaaatcaa caagatacac
    25321 aaacatttat catgcaatct tcgcgaacaa aaatgtataa tcaagaatat tatgttgttt
    25381 tattagattc aattcaaaaa gtagttgaga aaaaagaaaa agaagcttgg atcaatttaa
    25441 tgaaagtgat ttcgcatgaa cttttaaatt cattgacacc aattcgttca ttgtcgcaaa
    25501 atcttcatga attaattcaa caagagcact taagtcaaga agatttacaa gatattaagg
    25561 acagcgtaat tactatgcat aaccgaagca atcacttgca agaatttgtt gaaagttatc
    25621 gaaaactagc aatgttgcct tctccaaaaa aagaaaaaat agaactcact actttaacag
    25681 aaaattgtct tcaaattatg aaacctcttt tcaaaaaaga agcaattgaa attaaaaata
    25741 atatcgattt taatcgttgg attttggttg atccaaatca aatggagcaa gtttttatca
    25801 atttgctaac gaatagtatg tttgcgttgc aggataaaga aaataaagaa atcacaatta
    25861 gtgccgaaca taaagataaa cgactttata tcaacataac tgatacagga aacggaatag
    25921 agtcagaaat tgaagataaa atatttcttc ctttttttac tacaaggaaa gaaggcgcag
    25981 gaattggatt gactttatca aaaaatatag tagaagcgca cggtggatat ttggctttta
    26041 aatcggatga aaacaaaaca caatttacgg tttgcttagt ggagtaaact tgtaattatt
    26101 tgtaaatttt tgaaaaatta gaaagtaatt acaaattcat cttcggctcc caaaaatgtt
    26161 tttcaaaatc tatgatttga ttgtcaatta cttttatgcc ttcattttcg agtaattgct
    26221 gcattaaatt tgttccatca aaatgatgtt tccctgtcaa taatcctttt ctattcacta
    26281 cacgatgtgc aggaacatct tccaaattat gacttgcgtt cattgcccaa ccaaccattc
    26341 ttgcagatct tgctgcgcca agtgctttgg ctattgcacc atatgaagtt acttttcctt
    26401 cgggtattaa acgagcgatt tcataaacac
//
LOCUS       DAOOBH010000023        23188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_2880_length_23188_cov_4.725327, whole genome shotgun sequence.
ACCESSION   DAOOBH010000023 DAOOBH010000000
VERSION     DAOOBH010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 23188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23188
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2880_length_23188_cov_4.725327"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PK218_04275"
     CDS             complement(<1..191)
                     /locus_tag="PK218_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPW97758.1"
                     /translation="MRMDTIDKKLLTLLQQDSKKTTKELSLKLNLSVTAVYERIKKLE
                     REGIIAKYIALLDRTKIDK"
     gene            373..1575
                     /locus_tag="PK218_04280"
     CDS             373..1575
                     /locus_tag="PK218_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW97759.1"
                     /translation="MSTFNPADKIQDLQYFGEFGGVNPSISDSSTYTFLSAKTMFDTF
                     EGNAEGCYLYSRHSSPSNLYLGQALAAMEGTEAANVAASGMGAITPVLMQLCSAGDEI
                     VSSRTIYGGTYAFMKNMMPRWNIKTNFVDITKLDVVEAAITPNTKVLYCETVSNPLLE
                     VADIESLSKIAKKHNIALVVDNTFSPLSVSPIKLGADVVIHSLTKFINGSSDTVGGVI
                     CATQAFIDDLKNVNSGASMLLGPTMDSFRSASILKNLRTLHIRMKQHSHNAMHLAQAF
                     EKEGLKVVYPGLESHPSHAIYSSMINHEYGFGGMMTIDVGSLEKANSLMELMQNRNLG
                     YLAVSLGFYKTLFSAPGTSTSSEIPIEEQKEMGLTDGLIRFSIGLDNDIERTYQVMKQ
                     CMMELNIL"
     gene            complement(1630..2019)
                     /locus_tag="PK218_04285"
     CDS             complement(1630..2019)
                     /locus_tag="PK218_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97760.1"
                     /translation="MNDDTAQKKRHGCLTAWLIMMIIGNALGSFAYLILEPNVLAKED
                     IIMTKEQMVLLGILGFINLSFAIGLWYWKKWAFYGFALSGFLMFITNLNMGMDFVSSA
                     LGMVGVMLLFSILQMKQGETSGWDNLE"
     gene            2125..2568
                     /locus_tag="PK218_04290"
     CDS             2125..2568
                     /locus_tag="PK218_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97761.1"
                     /translation="MEFFYQGINLPASIDDEPGSHGYIAFKIKPKNTVVLNDVIENTA
                     NVYFDYNYPIITNTVSTTVTALKNISFENASFGLYPNPAKNLLNINISDATALKTISV
                     YNLVGQKILSFKVANSIDVSVLPQGSYLLTLETINGKATQRFIKL"
     gene            complement(2595..3941)
                     /locus_tag="PK218_04295"
     CDS             complement(2595..3941)
                     /locus_tag="PK218_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPW97762.1"
                     /translation="MLKRLLFIFAFLIAFTNYAQSGFKIKKNRNKVVIPFKLINNLVF
                     IPIKLNGEELTFLLDTGVEETILFSVNDNSELTLNDVEVIKLKGLGNSDAINGYKSSK
                     NEINIKQFVDSNHSLYIVLDQEFNFSSQVGIPVNGILGYHFFKNNLVKIDYGKRKIYV
                     YKKGHKVYRQLLNTYKKDSLIIDFNKPYVFETITNKGVKKYSKLLIDSGNSESVWVFN
                     KDKVLIPMSDHYLEDFLGRGFNGDVFGKRSRIEQIEFGGHQFKEVITNFPDSISTKSV
                     NLVDHRVGSIGGEILSRFTLFFDYPNNVMYTKPNVTVDEPFNFNMSGIEVEHTGLQWI
                     KEEYSSAKDGITIYTNTRASDYGTINENYQNSLKTRFSLVPVFKILSVRPNSEAEIAG
                     LKRGDKIISINNKNADRLSIQKIHELLKSGEGRTIQMKVERNGIEMKIKFQLKKVL"
     gene            4072..5268
                     /locus_tag="PK218_04300"
     CDS             4072..5268
                     /locus_tag="PK218_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPW97763.1"
                     /translation="MPRISKKGQWMPESPIRKLVPYAEMAKKKGHKVYHLNIGQPDIK
                     TPKCAIDAVKNTDITVLEYSHSAGFESYRKKLSAYYQTHGLPVETEDIIITTGGSEAL
                     MFAIGSTMDHGEEIIIPEPFYANYNGFATASGVTVVPVTTTLENNFELPPISEFEVLI
                     TPKTKAILICNPGNPTGRLYTYEEIVLLAEIVKKHDLFLIADEVYREFVYDVNEKHFS
                     VLNIEGIEQNAIMIDSVSKRFSMCGARIGCIVSKNKEVMEAAMKFAQARLSPPTFAQI
                     ASEAALETPQSYFDEVTIEYKSRRDTLVRELNKIEGIEVGVPSGAFYCIARLPVENAD
                     DFARWLLESYDSNGETVMIAPAEGFYSTQGLGLDEVRIAYVLKNEDLIRSVEILKEAL
                     KVYKAR"
     gene            complement(5500..5573)
                     /locus_tag="PK218_04305"
     tRNA            complement(5500..5573)
                     /locus_tag="PK218_04305"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5537..5539),aa:Arg,seq:tct)
     gene            complement(5584..5658)
                     /locus_tag="PK218_04310"
     tRNA            complement(5584..5658)
                     /locus_tag="PK218_04310"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5621..5623),aa:Pro,seq:tgg)
     gene            complement(5692..5775)
                     /locus_tag="PK218_04315"
     tRNA            complement(5692..5775)
                     /locus_tag="PK218_04315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5741..5743),aa:Ser,seq:gct)
     gene            6029..7207
                     /locus_tag="PK218_04320"
     CDS             6029..7207
                     /locus_tag="PK218_04320"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPW97764.1"
                     /translation="MNKKVVIVSAVRTPIGSFMGSLSSISATQLGAIAIKGALNKINL
                     DPKLVDEVLMGNVVQAGVGQAPATQASILSGIPSSVPSTTINKVCASGMKAVIQGAQA
                     IMTGDAEIVVAGGMENMSLIPHYVHLRNGVKFGPTSLVDGMQKDGLTDAYDNNAMGVC
                     ADLCASEYNISREEQDAYAIQSYERSAKAWDEGKFDKEVVPVEIPQRRGDAIIFSKDE
                     EYSNVKLDKIPTLNAVFTKDGTVTAANASTINDGAAALVLMSEEKAKTLGLKPLAYIK
                     SYADAAQEPKWFTTAPAKALPKALDKAGISLSDVDFFEFNEAFAVVGLANAKILGLDN
                     AKVNVNGGAISLGHPLGCSGARIIVTLLNVLEQNNAKIGAAAICNGGGGASAIVIERA
                     "
     gene            7295..8056
                     /locus_tag="PK218_04325"
     CDS             7295..8056
                     /locus_tag="PK218_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPW97765.1"
                     /translation="MFAICNLAIIPLRAEPSDRSEIVSQILFGEHFEILEQQKQWSKI
                     KLHFDNYEGWVDSKQYQFISEKNYKELSNDGIILNSDLVEYVTSANNVLVPITLGASL
                     SFLNHDDINVNQFEFEGLRVSGIKPKKEIVKTSFMYLNAPYLWGGKTPFGIDCSGFTQ
                     MVYKLNGYILLRDASQQASQGEALSFIEESEPGDLAFFDNEDGNIIHVGIIMEDNYII
                     HASGKVRIDRLDHLGIYNAEQNRHTHRLRVIKKIV"
     gene            complement(8126..10813)
                     /locus_tag="PK218_04330"
     CDS             complement(8126..10813)
                     /locus_tag="PK218_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HPW97766.1"
                     /translation="MKDEEEDNINPEEENQEQESHGFDEIKTSGHHFYEVDENPEETI
                     TKVTGMYKDWFLDYASYVILERAVPAIEDGFKPVQRRIMHSMKELDDGRYNKVANIVG
                     HTMQYHPHGDASIGDAMVQIGQKDLIIDMQGNWGNILTGDSAAASRYIEARISKFGHD
                     VLYSPKITEWGMSYDGRRAEPINLPVKFPLLLAQGAEGIAVGLSTKILPHNFNELIDC
                     SIKVLKGKSFTLFPDFPTAGIADVSNYNDGMRGGRVRVRAKIAQLDKQSLVITQIPFS
                     TNTSTLIDSILKANEKGKIKIKKIEDNTAAEVEILIHLPPGVSPDKTIDALYAFTACE
                     TSVAPLGCVIENHKPLFIGVTDMLKISTNRTVDLLKRELEIQLDELENKWHFSTLEKI
                     FIREEMYIDFKLYSDRESLYVYMYDRFKPFLKSFVREINDEDLQKLTQIPMIRITRFD
                     SDKADDAIAKLEAEMEQVKYHLDHIIDFAIDFFQKLKDKYGKGRERQTELRSFDTIEA
                     TKVVLRNTKLYVNREEGFFGTGLKKDEYVADCSDIDDVIVFLRDGKMMVAKVDDKKFV
                     GKDIIHIAVFDKNDKRTIYNMIYRDGKNGSTFIKRFNVSGVTRDKFYDLTQEKPGSQV
                     LYFSFNPNGEAETVTILLRQVGNIKKLKWDVDFADIAIKGRASRGNTVSKYPIKKIEL
                     KEKGISTLRPRKVWFDDTVQRLNVDGRGELLGEFRPNDRLLIINQSGKLKTIIPELTT
                     HFEEDMIVLEKWNPKKPISCIYFDGEKERYYVKRFLVENENKEEIFISEHDKSQLEIV
                     STDWLPMAELVFAKVKGIQKENQSINIEEFIAVKGIKALGNQLTTEKLKQVNLLEPLL
                     YEEPEEEAPEEMEVSGENNVSDDIQTELDDDGQITLSLE"
     gene            complement(10869..12725)
                     /locus_tag="PK218_04335"
     CDS             complement(10869..12725)
                     /locus_tag="PK218_04335"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPW97767.1"
                     /translation="MADETKYDEDNIRSLDWKEHIRMRPGMYIGKLGDGSSPDDGIYI
                     LLKEVIDNCIDEFVMGAGKVIEVSIKDKMVSVRDYGRGIPLGKVVDVVSKMNTGGKYD
                     SKAFKKSVGLNGVGTKAVNALSNFFRVESVRDNQIKAAEFSAGNLTLEEDVQESTKRK
                     GTKVSFIADEIIFKNYKYRNEYIIKMLKNYCYLNRGLTIYYNGEKYISENGLRDLLDE
                     NIAEEDKVYPIVHLEGEDIEIAITHSKSQYSEEYHSFVNGQNTTQGGTHLGAFREAIV
                     KTIKEFYNKPFEASDIRKSIVSAISIKVEEPVFESQTKTKLGSTDIGPKGPTVRTFVN
                     DFIKTKLDNFLHKNPEVADLLLRKILQAERERKELSGIRKLAKDRAKKANLHNKKLRD
                     CRVHLTDAKNPRSLESTLFITEGDSASGSITKSRDVNTQAVFSLRGKPLNSYGMSKKI
                     VYENEEFNLLQAALNIEEDMGDLRYNNIVIATDADVDGMHIRLLLITFFLQFFPELIK
                     DGHLYILQTPLFRVRNKKETIYCYSEPERVDAIEKLKPKPEITRFKGLGEISPDEFKH
                     FIGESIRLDPVMLDKATSIETLLEFYMGKNTPDRQEFIINNLKVELDVVEKN"
     gene            complement(13198..14291)
                     /gene="ychF"
                     /locus_tag="PK218_04340"
                     /pseudo
     CDS             complement(13198..14291)
                     /gene="ychF"
                     /locus_tag="PK218_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
     gene            complement(14373..15539)
                     /locus_tag="PK218_04345"
     CDS             complement(14373..15539)
                     /locus_tag="PK218_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97768.1"
                     /translation="MSVKGLLAILFFYSIQLFSQTESKTIILKDKDTDAPIEDVTVTL
                     LKTKQNLLSNANGIVTFELTGSTNIQIKHSSYLPINLRSNTLKETVNTIYLTSNVNEL
                     EDIVITKKHPQKILASLIENSKKRLTVPGRLKVYCREFFKLNGKYSYFNDGLMNIQLL
                     NKSRKTDAIILVEQNRSINLTNDFVKDDLLGYNLNDIMENYYNFKYLNPLLDAVAKKE
                     YDFVIKVFSKNKDYNVISAVPNENASGLLDDFTIIYDPDKKLIIEVSSIVSPLVLSKI
                     KEKTSLGSKNIYKSMFKTIYKLQNNNYYLIGSKEEIGFEKNEKNAKKDIEVRNYFLTK
                     NFSTSYYKFKEEEVFKDKTLHNKKNVILTDYWNESGLTATEDEQQIINTIEEIH"
     gene            15726..16259
                     /locus_tag="PK218_04350"
     CDS             15726..16259
                     /locus_tag="PK218_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97769.1"
                     /translation="MKNLISIAFLLLVTFSLQAQNKNVLKETETKTTTVKNSEGEKKI
                     VTTNETEEVQNIQLEEAKPNTLNIPMKESPVMVTKTTELSVDGKTKYVDVDHSAYYMN
                     NGTKYQFKTDDYGYSIINNNSKKNSYLRKTSNNNYLFVNDGKVSSAYFDADGNLIIDT
                     YNEKTDRITTEKFMVAK"
     gene            complement(16318..16668)
                     /locus_tag="PK218_04355"
     CDS             complement(16318..16668)
                     /locus_tag="PK218_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPW97770.1"
                     /translation="MAIIITDDCINCGACEPECPNTAIYEGADDWRYKDGTQLKGKVI
                     LPDGTEVDADIAQTPISDDIYYIVPGKCTECKGFHEEPQCAAVCPVDCCIPDDNHVES
                     EETLLNRQAFLHNE"
     gene            16795..17868
                     /locus_tag="PK218_04360"
     CDS             16795..17868
                     /locus_tag="PK218_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HPW97771.1"
                     /translation="MLQLDKKECFIALGKFLSQFSMDANSKNETVLYNDLFYDDFIDL
                     IHLSQSHNGWFTPEQVYFALNSWAEALTDKNLSLWLSKYSFDCAQDDKKKVKTVGLIL
                     AGNIPLVGFHDFLSVLISGHNVLVKTSSNDQHLIKFLAKYLIAVNPEIKNSITFTDGK
                     LEDFDAVIATGSNNTARYFEFYFKDKPSIIRKNRNSIAVLDGNETHEDLVNLGEDIFR
                     YFGLGCRNVSKLFVPKGYDFKNFFEAIYKYKDVIFYEKYSNNYDYNKAVFLMSNFKLL
                     DNEFLTLKEDPSYASPISSVFYENYEDLETLKSRLELEKEQIQCIVSNDLIPKSIAFG
                     QTQKPQLWDYADSVDTLEFLINL"
     gene            17984..19054
                     /gene="serC"
                     /locus_tag="PK218_04365"
     CDS             17984..19054
                     /gene="serC"
                     /locus_tag="PK218_04365"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPW97772.1"
                     /translation="MKKHNYSAGPCILPQEVFEKSAEAILNFNNSNLSILEISHRSKD
                     FVAVMDEARALVLELLGLEGKGYQALFLQGGASMEFLMVPYNLMNVDGKAAYLDTGTW
                     ASNAIKEAKLFGGTEIVASSKEANYNFIPKEYAIANDASYFHCTSNNTIFGTQMKSFP
                     QTNVPLVCDMSSDIFSRTLDFSKFDLIYAGAQKNMGPAGTTLVVVKEAILGKSGRTIP
                     SILDYEKHIKAESMFNTPAVFPVYASLLTLQWLKKLGGIAAIEKINEAKANLLYNEID
                     RNPLFKGAANKEDRSIMNATFLLENEAHQETFDKMCKDAGISGINGHRSVGGYRASMY
                     NALPIESVQVLVAVMQELERNV"
     gene            19115..20077
                     /locus_tag="PK218_04370"
     CDS             19115..20077
                     /locus_tag="PK218_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HPW97773.1"
                     /translation="MKILANDGISKSGIKALEKGGFEVITTKVAQEQVANYINSNNVS
                     VILVRSATKVRQDIIDNCPSLKIIGRGGVGMDNIDVEYARSKGIHVINTPASSSESVA
                     ELVFAHLFTGVRFLHDANRNMPLEGDSNFDGLKKAYANGIELRGKTLGIIGFGKIGQA
                     VAKMAVGLGMKVIASDKFVGKADVRVDFYNGQFINVEIETEPLEDLLRHSDFITLHVP
                     AQDTYVIDKDELAIMKDNVGIINCARGGVLNEVALIEALENNKVLFAGLDVFEKEPSP
                     EIQILMHPRISLTPHIGAATLEAQDRIGTELAEQIISILKTEKV"
     gene            20277..20720
                     /locus_tag="PK218_04375"
     CDS             20277..20720
                     /locus_tag="PK218_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97774.1"
                     /translation="MDGLKGMVANGNVSDLVGLFQGNTAIDSSNPVVQQLTQKFSGNL
                     GQKFGLSNEASSGVAASLIPKVLGSLIGNAKDPNHKGFEVSDIINAISNGQGNSGLMD
                     AISNYGGQLGLDQNGDGKVGMDDAIAAVSNKSGGLGGMLGKLFGK"
     gene            20829..21254
                     /locus_tag="PK218_04380"
     CDS             20829..21254
                     /locus_tag="PK218_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6146 family protein"
                     /protein_id="HPW97775.1"
                     /translation="MKNLIPFIVIIAIVIGCNSSKSGFNATDSLVANDTIKIENDSLE
                     YQVIIIDTGFNSWLVSTAKPRGYYSERYLEDRNYIYVQEWNNRVNQPSRYGIDLYEMR
                     IDYDRNIHYGYEVNYLIYNYMIYFQIKNNQRLSGFVPRP"
     gene            21281..21598
                     /locus_tag="PK218_04385"
     CDS             21281..21598
                     /locus_tag="PK218_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglyceryl transferase"
                     /protein_id="HPW97776.1"
                     /translation="MASFKERWNITSNTQMVVILVVFAITGSSSALLSKPILAVFGII
                     KGDVSNWIYYPLYILLIFPVYQVLLISFGFLFGQFKFFWAFEKKMLKSLGFGFFFKKE
                     NLE"
     gene            complement(21646..22323)
                     /gene="folE"
                     /locus_tag="PK218_04390"
     CDS             complement(21646..22323)
                     /gene="folE"
                     /locus_tag="PK218_04390"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPW97777.1"
                     /translation="MNTTIEKELIELMGDNHHMTSANTPLRADAFHKNDNEKMMVIED
                     HFHAIMEVMGLDMTDDSLKGTPHRVAKMFIKEIFSGLNPENKPKISVFENSYHYHKML
                     VEAAISFNSTCEHHFLPIIGKAHIGYVSSGKVIGLSKLNRIVDYYSRRPQVQERLIMQ
                     IFNELKTVLDTDDVIVVMEAKHLCVSSRGIQDESSFTSTIQYGGIFNEKENRNDFFNL
                     VGKQKQN"
     gene            22471..23181
                     /locus_tag="PK218_04395"
     CDS             22471..23181
                     /locus_tag="PK218_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW97778.1"
                     /translation="MITANNIFKNYDALQVLKGVTLHIEKGEIVSIVGASGAGKTTLL
                     QIIGTLDHPNTNKETTLEINGKNILKMDDATLSKFRNEHLGFIFQFHQLLPEFTALEN
                     VCIPAYIAKKEKTPTEAEAKRLLDYLGLSHRIHHKPNELSGGEQQRVAVARALINKPA
                     VIFADEPSGNLDSTSAENLHELFFKLRDEFGQTFVIVTHNEALANMADRKLTMVDGNI
                     DTNVDWIDWKAINNIETN"
BASE COUNT         7505 a         3829 c         3911 g         7943 t
ORIGIN      
        1 cttttatcta ttttagttct gtctaatagc gcaatatatt tggcaattat accttctctt
       61 tctagctttt ttatccgttc gtaaactgcg gttacggata aattgagctt taatgataac
      121 tctttagtgg tctttttaga gtcttgttgc agcaaggtca aaagtttttt gtctatagtg
      181 tccatacgaa ttgattatag gtttgatgtt tctacttttc tttaaaaagg ataaaaatcg
      241 tatttaaata gaatataaat ctacaattaa caacattaat agactaataa tctaaatttt
      301 ttcaaatgta ttgaaaaata aattaataat attcaatttt gaataacatt aaaaacataa
      361 accacacaaa ttatgagtac ttttaatccc gctgataaaa tacaagacct tcaatacttt
      421 ggagagtttg gaggcgttaa tccttctata tcggatagtt caacttatac gtttctttcc
      481 gcaaaaacta tgtttgacac ttttgaaggc aatgcagaag gttgttattt atattcgcgc
      541 cattcatcac catcaaattt atatttagga caagcacttg ccgctatgga aggcaccgaa
      601 gctgctaatg ttgccgcatc gggaatgggt gctattacac ctgtattgat gcaattgtgt
      661 agtgctggcg atgaaatcgt atcgagtaga acgatttatg gcggtactta tgcctttatg
      721 aaaaacatga tgccacgatg gaacatcaaa accaattttg tagatattac taaattagac
      781 gtggtggaag ctgcgattac tccaaacaca aaggttttat actgtgaaac agttagtaat
      841 ccattactgg aagttgccga tattgaaagt ctctctaaaa tagccaaaaa gcacaatatt
      901 gcgctagtgg ttgataatac cttctcgcct ctttcagttt ctccaataaa actaggtgcc
      961 gatgtagtga tacacagttt gactaagttt atcaatggta gtagcgacac agttggtggt
     1021 gttatttgtg ctacacaagc gtttatcgat gatttaaaaa atgtaaatag tggcgcaagt
     1081 atgttgctag gaccaacaat ggatagtttc cgttcggcaa gcatcttaaa gaaccttcgc
     1141 accttgcaca tccgcatgaa acaacacagt cataatgcga tgcatttggc acaagcgttt
     1201 gaaaaagagg gtttgaaagt agtatatcct ggattagaaa gccacccaag tcatgctatt
     1261 tattcgtcaa tgattaatca cgaatatggt tttggaggca tgatgaccat tgatgttggc
     1321 tcgttagaaa aagctaattc attgatggaa ttgatgcaga accgaaattt aggttatctt
     1381 gcggttagtt tagggtttta taaaacccta ttttctgcac ctggaacctc aacatcctca
     1441 gagattccga ttgaagaaca aaaagaaatg ggcttaacag atgggttaat tcgtttttca
     1501 ataggcttgg ataatgacat cgaacgcacg tatcaagtta tgaaacagtg catgatggaa
     1561 ttgaatattt tataattttt tttttagttt ttttgaaatc cgtaagttaa tcgacaattt
     1621 acggattttt tattccaaat tatcccaacc cgaagtttcc ccttgtttca tttgaagaat
     1681 agaaaacaaa agcatcaccc caaccattcc taatgctgaa gaaacaaaat ccatacccat
     1741 attaagattg gtaataaaca ttaaaaaacc cgaaagtgca aaaccataga aagcccactt
     1801 cttccaatac caaagaccaa ttgcaaacga caaattaata aatcctaaaa tacccaataa
     1861 caccatctgc tctttggtca tgatgatgtc ttctttggct aatacatttg gctcaagaat
     1921 aagatatgca aaagaaccta atgcatttcc aattatcatc atgattaacc aagccgtaag
     1981 acagccgtgt cttttctttt gtgctgtatc gtcgttcata ggggttttgt ttgtcgcaag
     2041 caaaattaca ttttttattt catttgctaa aaagttaaaa attcagttaa taaaatatag
     2101 tagtaaaaaa caggaggcaa taagttggag tttttttatc aaggtatcaa tcttccagcg
     2161 tctatagacg atgaaccagg cagtcatggt tatattgcgt ttaaaataaa gccaaaaaac
     2221 acggtagttt taaacgatgt aatcgagaat actgccaacg tctattttga ttacaactac
     2281 ccaataatca ctaatactgt cagcacaacg gttaccgcgt taaagaacat tagcttcgaa
     2341 aacgcttcgt ttggcttata tcctaaccct gctaaaaatc tattgaacat aaatatttct
     2401 gatgcaacag ccctaaaaac catttcagtt tataacttgg ttggtcaaaa aatactatcg
     2461 tttaaagtcg ccaattcaat tgatgtgagc gtattgccac aaggaagtta cttgcttact
     2521 ttagaaacta ttaatggaaa agcgacgcaa agatttatta aattataatc caagatacgc
     2581 ttttgagtgt tggtttacaa cacctttttt aattgaaatt tgattttcat ttcaattccg
     2641 ttgcgctcaa ctttcatttg aatagttcgt ccttctcccg atttgaggag ttcgtgtatt
     2701 ttttggattg atagtcggtc tgcattctta ttattgatac tgattatttt atcacctctt
     2761 tttaaccctg caatttcagc ttccgaatta ggtctaacac ttaatatctt gaaaaccggc
     2821 actaaactaa aacgcgtttt tagactattt tggtaatttt cgtttatggt accataatct
     2881 gaagctcttg tgttagtgta aattgttatt ccatctttag cggaagaata ttcttctttt
     2941 atccattgta gtcctgtatg ctctacctct attccactca tattaaaatt aaatggttca
     3001 tcaacagtaa cattgggttt ggtgtacata acattattag gataatcaaa aaaaagagta
     3061 aatctagaaa gtatttctcc accaattgaa ccaactctat gatcaactaa attgacgctt
     3121 tttgtagata ttgaatcagg aaaattagtt attacttctt taaattgatg accaccaaat
     3181 tcgatttgtt caatgcggct tctttttcca aacacatcac cattgaaacc tcttcctaaa
     3241 aaatcttcta aataatgatc agacattgga atgagaactt tatctttatt aaaaacccac
     3301 actgattcac tattaccgct atcaatcaat agcttagagt actttttaac acccttgttt
     3361 gtaatggttt caaaaacata aggtttgtta aaatcaataa tgagagaatc ttttttatag
     3421 gtgtttaaaa gttgacggta aactttgtgt ccttttttat aaacatatat tttgcgctta
     3481 ccataatcaa tttttactaa attattctta aaaaaatgat aacccaaaat accgttaacc
     3541 ggaataccaa cttgagaaga gaagttaaat tcttggtcga gaactatgta taaggaatga
     3601 ttactatcaa caaattgttt gatgttaatc tcatttttgg aagacttata accattaatt
     3661 gcgtcgctat taccaagccc ttttaacttg ataacttcaa catcatttaa agttaattca
     3721 ctattatcgt ttacactaaa gagaatggtt tcttcaacac ctgtatcaag caaaaaagtc
     3781 aattcttcac catttaattt aattggaata aaaactaggt tgttgattaa cttaaatgga
     3841 ataactactt tgtttctatt ctttttaatt ttaaaacccg attgagcata gttcgtaaaa
     3901 gcaattagaa aggcgaatat aaataatagt cttttgagca ttaaattaat tttttataaa
     3961 attaataata attctataac aattagattt attaacatca tttaaaggta gtatttgatt
     4021 tttttaaacg ctaaaaaaag tgcaaatttg cagaatcaaa tttttgaaat catgccaaga
     4081 atatctaaaa aagggcaatg gatgcctgaa tcaccaatta gaaagttagt tccttatgcc
     4141 gaaatggcaa aaaagaaagg acataaagta tatcatttaa atattggaca acccgacatc
     4201 aaaacgccaa aatgcgctat agatgcggta aaaaatactg atattacagt gttagaatac
     4261 agtcattctg cagggttcga aagttataga aaaaaacttt ctgcatatta tcaaacccat
     4321 ggactacccg ttgaaacaga agatatcatc attacaactg gtggttccga agctctgatg
     4381 tttgcaatag gaagcactat ggatcatgga gaagaaatta tcattcctga accattctat
     4441 gccaactata atggttttgc tacagcttca ggagtaacgg ttgttcctgt aactactacc
     4501 ttggaaaata actttgagct gcctcctata tcggaatttg aagttttaat tactccaaaa
     4561 actaaagcaa tattaatttg taacccagga aacccaaccg gaagattata tacttatgaa
     4621 gaaattgtgc ttttggcaga gattgttaaa aagcatgatt tgttcttgat tgctgatgaa
     4681 gtttacagag aatttgtgta tgatgttaat gagaaacatt tttcggtttt aaatattgaa
     4741 ggcattgaac aaaatgccat catgattgat tcggtttcaa aacgttttag tatgtgtggt
     4801 gcacgtattg gatgtattgt ttcaaaaaac aaagaggtaa tggaagctgc catgaagttt
     4861 gcacaagcaa ggctttctcc tccaacattt gctcaaattg ccagtgaagc tgctttagaa
     4921 actcctcaaa gttattttga tgaagtgact attgaatata aaagccgcag agatactttg
     4981 gtaagagaat taaataaaat tgaaggaatt gaagtaggtg ttccgagtgg tgctttttat
     5041 tgtatagcac gattgccagt agaaaatgct gacgattttg ctcgttggtt gttggaatct
     5101 tatgacagca atggcgaaac agtaatgatt gctccagctg aaggttttta ttctactcaa
     5161 ggattaggat tggacgaagt gcgcattgct tatgttctta agaatgaaga tttaattcgc
     5221 tcagtagaga tattgaaaga agcattgaaa gtatataaag caaggtaatt tctacttaaa
     5281 aacattaaga aaccttctca aatgagaagg ttttttgttt ctaagacaaa taaaaatcat
     5341 tgggttaccc tacataaata gagattgtat aaagttcaat tttcgtccgc aaaaagtgtg
     5401 caaaagtgtt catttattac cgacagatgt ataaaattaa tttcaataca tcatcaccaa
     5461 aaacaacaaa accccactat tagtgaggct ttaaacttgt gatctcagaa ggattcgaac
     5521 cttcgaccta ctgcttagaa ggcagttgct ctatccagct gagctatgag accatttttt
     5581 ttgtcggggt ggcaggattc gaacctgcga cctcctgctc ccaaagcagg cgcgataacc
     5641 gggctacgct acaccccgtg tatttattat taggttacac ttcctaaaaa gcggagagac
     5701 agggactcga accctggcga cagttacccg tcgacagatt agcaatctgc tccattaccg
     5761 ctctggcacc tctcctatat tttcaatctt tggtaagaac tttttttctt aaagcggttg
     5821 caaatgtact ttatcattct ataatttaca actatttcat ttcttttttt gcagttaatt
     5881 ttaattttat attgaatgtt ttaaaattca atgaaataga atagtatttg atttttttat
     5941 ccgcttaaaa aagatattaa tttgcctaac aattccatat attgcaataa aagattagtt
     6001 ttgcagtaca ataaaactaa actataccat gaataaaaaa gttgtaatcg tatcggctgt
     6061 aagaactcca attggaagtt ttatgggttc tttgtcttct atttctgcta ctcaattagg
     6121 agctattgca ataaaaggag ccttaaataa aataaatctt gatcctaaat tggttgatga
     6181 agtgttaatg ggtaatgtag ttcaagcagg tgttggacaa gcaccagcaa cgcaagcatc
     6241 cattctctca ggaatcccta gctcagttcc ttcaacaaca ataaataaag tatgtgcttc
     6301 tggaatgaaa gctgtaatac aaggtgctca agctatcatg acaggtgacg ccgaaattgt
     6361 tgtcgctggt gggatggaaa acatgagttt gattccacat tatgtccatt taagaaatgg
     6421 tgtgaagttt ggtccaactt ccttagtcga tggtatgcaa aaagatggat taacagatgc
     6481 ttacgacaat aacgccatgg gtgtttgtgc cgatttatgt gcatccgaat acaatatttc
     6541 tcgtgaagag caagatgctt atgccattca atcctacgaa cgttctgcaa aagcttggga
     6601 tgaaggaaaa tttgacaaag aagttgttcc agtagagatt ccgcaacgtc gtggagatgc
     6661 tattattttt tctaaagatg aagaatatag taatgtaaag ttggacaaaa tacctacttt
     6721 gaatgctgtt tttactaaag atgggacagt tacagctgcc aatgcttcta ctattaatga
     6781 tggtgctgct gctttagtat tgatgagcga agaaaaagca aaaactttag gattaaaacc
     6841 tttggcatat ataaaatcat atgcagatgc agcacaagaa ccgaaatggt ttaccacagc
     6901 acctgcaaaa gcattaccaa aagcgttaga caaagctggt atttcacttt ctgatgtaga
     6961 tttctttgag tttaatgaag cttttgctgt tgttggttta gcaaatgcta aaattttagg
     7021 tttagacaat gctaaagtca atgtaaatgg tggtgcaatt tctcttggtc atcccctagg
     7081 ttgttctgga gctagaatta ttgttacatt attaaatgtg ctagaacaaa acaacgcaaa
     7141 aattggagca gcagcaatct gtaacggtgg tggaggtgct tctgcaatag tcattgaaag
     7201 agcttaaaaa attataagtt atgcgttata aatttttaag tttatgatac tttgcaattt
     7261 ttaactcata attcaaaact cataattcta ttaaatgttt gccatttgta atttagcgat
     7321 tattccttta cgtgctgaac ctagtgatag aagcgaaatc gtttctcaaa ttttgtttgg
     7381 agaacatttt gaaattttag aacagcaaaa acaatggtct aaaatcaaat tgcattttga
     7441 caactacgaa ggctgggtag atagcaaaca ataccaattc atttcggaaa agaattataa
     7501 ggaactttct aacgatggta ttattctaaa ttcagattta gtggaatatg taaccagtgc
     7561 taacaatgta cttgttccta ttactttagg agcatcatta tcgtttttaa atcatgatga
     7621 tataaatgtt aaccaatttg aatttgaagg attacgcgtt agtggaataa aaccaaaaaa
     7681 ggaaattgta aaaacttctt ttatgtattt gaatgcacct tatctttggg gcggaaaaac
     7741 accatttgga attgattgtt ctgggtttac acaaatggtt tataaattga acggctatat
     7801 tttacttcga gatgcttcgc aacaagcctc acaaggtgaa gctttgagtt ttattgaaga
     7861 aagtgagcct ggtgatttag ctttttttga caatgaagat ggcaacatca tccatgtagg
     7921 catcataatg gaagacaatt atataattca cgcaagcgga aaagtacgca tagaccgatt
     7981 agaccacctt ggcatctaca acgctgaaca aaacagacac acgcatagat tgagagtaat
     8041 taaaaagata gtataatttc caatataaaa ccaaaaagta aaaccctcat caatcgataa
     8101 gggtttttgt attgtagata gaaaactact ctaaagacaa agtgatttgt ccatcgtcgt
     8161 ctagctctgt ttgaatatcg tcggaaacat tgttttcacc agaaacttcc atttcttcgg
     8221 gtgcttcttc ttcgggttct tcatagagta acggctccaa aaggttgact tgttttagtt
     8281 tctcggtagt caattggttt cctaatgctt taattccttt gaccgcgatg aattcttcta
     8341 tattaatgct ttgattttct ttttgaatgc cttttacttt ggcaaaaaca agttcagcca
     8401 ttggaagcca atcggtagaa acaatttcta gttgcgattt atcatgttcg gatatgaaga
     8461 tttcttcttt gttttcgttt tcaaccaaga agcgttttac atagtagcgt tctttttcgc
     8521 catcaaaata tatgcaggaa ataggtttct tagggttcca tttttccaat acaatcatgt
     8581 cttcttcaaa atgggttgtc aactcgggaa tgatggtttt tagttttccc gattggttga
     8641 tgattagcag tcggtcattt ggtctgaatt cgcccaacaa ctctcctctt ccatctacat
     8701 ttaatcgttg cacggtatcg tcgaaccata cttttcgtgg gcgcaaagtt gaaattcctt
     8761 tttctttaag ttctattttt ttaattggat atttggaaac agtatttcct cgggaagcac
     8821 gtcctttgat agcaatatcg gcaaaatcaa catcccattt taatttctta atgttgccga
     8881 cttgtcgtaa taatatggta acggtttcgg cttctccatt tggattgaac gaaaaataca
     8941 atacttgcga acctggtttt tcttgggtta aatcgtaaaa cttatcacgc gttacacccg
     9001 atacattaaa tcgtttgatg aatgtagagc catttttgcc atcgcgataa atcatgttat
     9061 aaatggttcg tttgtcgttt ttatcaaaaa cagcaatgtg tataatgtct ttgcccacaa
     9121 attttttgtc gtccactttg gctaccatca tttttccgtc gcgaaggaag acaattacgt
     9181 cgtctatgtc cgaacaatcg gcaacgtatt cgtctttctt taaccctgtt ccgaagaaac
     9241 cttcttccct atttacgtat agttttgtat ttcttaaaac aacttttgtc gcttcaatag
     9301 tatcaaagct tcgcaattct gtttgacgtt ctctaccttt tccgtatttg tcttttaatt
     9361 tttggaaaaa gtctattgca aagtcgataa tatggtctaa atgatatttt acttgttcca
     9421 tttcagcttc caacttggca atagcatcat ctgccttatc cgaatcgaag cgagtgatac
     9481 gtatcattgg gatttgtgtc aatttctgta aatcttcatc attaatttca cgaacaaatg
     9541 atttaagaaa tggtttaaat cgatcgtaca tataaacata aagtgattct ctatcggaat
     9601 acaatttgaa gtcgatgtac atttcttctc gaatgaagat tttctccaat gtagaaaaat
     9661 gccacttgtt ttctaactcg tccaattgaa tttccaactc acgtttaagt aaatcaactg
     9721 ttcggttagt tgatattttc aacatatcgg taactccgat aaacaacggt ttatggtttt
     9781 caatgacaca gcccaatggt gcaactgatg tttcgcaagc cgtaaaggca tacaaagcgt
     9841 caatcgtttt atctggagaa acacctggcg gtaggtgaat tagaatttca acttcggcag
     9901 ctgtattgtc ttcaatcttt ttgattttta ttttgccttt ttcattggct ttcaaaatac
     9961 tatctatcaa agtagaggta ttggttgaaa aaggaatttg ggtaataacc aaagattgtt
    10021 tgtctaattg tgcaattttt gcccgcacgc gaactcttcc accacgcatt ccatcgttgt
    10081 agttggaaac atcggcaatt cccgcggttg gaaaatcagg aaataaagta aacgattttc
    10141 ctttcaacac tttaatggag caatcgatta gttcattgaa attgtgcggt aatattttgg
    10201 tagataaacc aactgcaata ccttcagcac cttgcgccaa caatagcgga aacttaaccg
    10261 gaagatttat tggttcggca cgacgaccat cgtatgacat tccccattcg gtgatttttg
    10321 gtgaatacaa cacatcatga ccaaatttgg aaatacgcgc ttctatgtaa cgagaagctg
    10381 cagcgctatc gcccgtgagg atgtttcccc agtttccttg catgtcgata atcaaatctt
    10441 tttgaccaat ttgaaccatc gcatcgccaa tactcgcatc accgtgtgga tggtactgca
    10501 tggtatgccc aacgatattg gctactttgt tgtatcgacc atcatcaagt tctttcatcg
    10561 agtgcatgat tcgacgttgt actggtttga agccgtcttc aatagcagga acagcacgtt
    10621 ctagaattac ataggaggca tagtccaaaa accaatcttt gtacattcct gtaactttgg
    10681 taatggtttc ttctgggttt tcatctactt catagaaatg atgtcctgat gtttttattt
    10741 catcaaaacc atggctttct tgttcttgat tttcctcttc tgggtttatg ttgtcttctt
    10801 cttcgtcttt catttatgct gaataattac tgcgtcagtc gctacattcg tgtcattgtt
    10861 tttttaatct aatttttctc cacaacatcc aattctacct tcaagttatt gattataaac
    10921 tcttgtctat ctggagtgtt tttgcccatg tagaattcta aaagcgtttc aatagaagta
    10981 gctttatcta gcatcacagg atccaaacgg atgctttcgc caatgaagtg tttaaactca
    11041 tctggagaaa tttcacccaa tcctttaaat cgggtaattt cgggttttgg ttttagtttt
    11101 tctatggcat caacacgttc tggttcgcta taacaatata tggtttcttt tttatttcta
    11161 acacggaata atggcgtttg caaaatatat aaatgaccgt ccttgattaa ttcagggaag
    11221 aattgtagaa agaacgtaat taataacaag cggatgtgca ttccatcgac atcggcatcg
    11281 gttgcaatta cgatattatt ataccgcaaa tcgcccatgt cttcctcgat gtttagtgct
    11341 gcttgaagca aattgaactc ttcgttttca tatacaatct tcttcgacat tccataggaa
    11401 ttcaaaggtt taccacgcaa actgaaaaca gcttgtgtat tcacatcacg acttttagtg
    11461 attgaacccg atgcagaatc accctcggta atgaacaagg tgctttctaa acttcttggg
    11521 tttttggcat cggttaaatg cactctgcaa tcacggagtt tcttgttgtg cagattggct
    11581 tttttagctc tgtctttggc tagttttcga attccagaaa gttctttacg ttcgcgttct
    11641 gcttgaagaa tcttgcgcaa taacaaatcg gcaacttctg ggtttttatg taaaaagtta
    11701 tcgagcttgg ttttgataaa atcgtttaca aaggttcgca cggttggacc ttttggacca
    11761 atatcggttg aacccaattt agttttggtt tgactctcaa aaacaggttc ttctactttt
    11821 attgaaattg cagaaactat tgattttctg atgtctgatg cttcaaaagg tttgttgtaa
    11881 aattctttaa tcgtttttac aattgcttca cgaaatgctc ctaaatgtgt tccaccttgc
    11941 gtagtgtttt gaccgtttac aaatgaatgg tattcttcag aatattgtga tttgctgtgt
    12001 gtaatggcga tttcaatatc ttcaccttct aagtgaacga ttggatatac tttatcttct
    12061 tctgcaatat tttcatctag taaatcgcgt aaaccatttt cggaaatgta tttttcacca
    12121 ttgtaataaa ttgttaatcc acgatttaga tagcaatagt ttttgagcat cttgatgatg
    12181 tactcgtttc ggtatttgta gtttttgaaa ataatttcat cggcaatgaa agatactttg
    12241 gttcctttgc gttttgtgct ttcttgaacg tcttcttcta gtgttaaatt acctgccgaa
    12301 aactcagccg ctttaatttg attgtcgcga acggattcta cacgaaagaa attggacaat
    12361 gcgtttacgg ctttggtacc aacaccattt aaaccaacgg atttcttaaa ggcttttgaa
    12421 tcgtactttc caccggtatt cattttggaa actacatcta ctacttttcc taatggaata
    12481 ccacgaccgt aatcgcgtac tgaaaccatt ttgtctttga tggaaacttc aatgacttta
    12541 ccagcaccca taacgaattc atcaatacag ttatcgataa cttcttttaa taatatataa
    12601 attccatcat cgggagaaga accatcgcct agctttccga tgtacattcc tggacgcatt
    12661 cggatgtgtt ctttccaatc gagtgagcga atattatctt cgtcgtattt agtttcgtca
    12721 gccattttaa aatttagtga atgtgtgcta atatagtgat tgccgataga tttttaaaat
    12781 ggattttgta gaagttatta agaatgatat tgacaatgtt ttagagaaaa caaaacaaaa
    12841 aaactagaaa atagattata gcttttaact tttacctcgc gctctaacac tctttactcg
    12901 gggttcaaca ctcttcactc gtgctccaac actcttcact cgtagtccaa cactttgtac
    12961 tcatagtcca acactttgta ctcacggtgt aacactcttc actcgtgctc caaccctcag
    13021 actggatact tcaacactca gtactcgagg ttcaacactc tatactcatg gtttaacacg
    13081 caattattat ttgttttttt gtgtttgaat tgatttattg aacgaaatgg tattgaatat
    13141 ataaaaaaac cgcaaaagag attttgcggt ttattattta ttttattttc ttatcgatta
    13201 cacattaaaa cggaaatgca tcacatcgcc atcttttacg atgtattctt ttccttctac
    13261 tctaagtttt cctgcttctt ttactttggc ttctgaaccg taattggaat aatcttcaaa
    13321 agcgattact tcggcacgga tgaatccttt ctcgaagtcg gtataattac tccggctgct
    13381 tttggtgctg tatcgccaat gtttattgtc caagcacgaa cttcttttac acctgctgtg
    13441 aaataggttt gtagttttaa taatttataa gctgcacgaa ttagtactgc tgaacctggc
    13501 tctgtcaatc ccatatcttc aaggaatact tgtcgttctt cgtagctttc taattctgta
    13561 atatctgctt ctgctcctac tgaaagaatg atcacttcgg cttcttcgtc ttttactaat
    13621 tcgcgaactt ggtcaacata tttgtttcca ttaactgctg cactttcatc aacattacaa
    13681 acataaagca caggtttgtt tgtaattaat tgaaaatctt ccatcaaaga ttcttcgtca
    13741 ttgttttgag gaataacggt tctggctgat tttgcttgaa gtaatgcttc acgaattcta
    13801 ttaagcaatg cttgttctac ttgtgcttct ttattacctg tttttgcggc gcgatttact
    13861 ttttctaaac gtttttcgac ggtttctagg tcttttaatt gcaattcgat gtctatggtt
    13921 tctttgtctc gaattgggtt tacatttccg tcaacgtgaa caatattgtc gttatcaaaa
    13981 caacgtaaaa cgtgaatgat tgcattacat tctctaatat ttgctaagaa ttgatttcct
    14041 aaaccttcgc ctttacttgc tccttttact aatcctgcga tgtcaacaat atcaacggtt
    14101 gccatttgaa cccgctctgg ttttactaat tcttccaatc gatttattct tggatcagga
    14161 acatttacta ctccaatatt tggttcgatg gtacaaaatg gaaagttggc actttgtgct
    14221 tttgcgtttg acaaacaatt aaataaagtt gattttccaa catttggtaa tcctacaatt
    14281 cctgctttca tattgtttta aattggtaat agggaacgca aaagcgtttc attataagtt
    14341 ttgatttaaa aaagtgtgca aatatagtct aattaatgaa tttcttcaat agtatttatt
    14401 atttgctgtt cgtcttcggt agcagttaaa ccagattcat tccaataatc tgtcaggatt
    14461 acatttttct tgttatgtag tgttttgtct ttaaagactt cttcttcttt gaatttataa
    14521 taactggtgc tgaaattttt ggttaaaaag taatttcgca cttcaatatc ttttttagcg
    14581 tttttttcgt ttttttcaaa accaatttct tctttggaac caattaaata gtagttatta
    14641 ttttgaagtt tataaatggt tttaaacatt gatttatata tgtttttgga gcccaaactt
    14701 gttttctctt ttatttttga taaaaccaat ggcgacacta ttgaactgac ttctataatt
    14761 agttttttat caggatcata aatgatggta aaatcatcta ataatcctga tgcattttca
    14821 tttggaacag ctgaaatcac attgtagtct ttgttttttg agaacacttt aattacaaaa
    14881 tcgtattctt tttttgccac tgcatcaagc aatgggttga gatatttgaa attataatag
    14941 ttttccataa tatcatttaa gttgtaaccc aacaaatcgt cttttacaaa atcgttggtc
    15001 aagtttatag agcggttttg ttctacaagg attatggcat ctgtttttcg tgatttattg
    15061 agtaattgaa tattcatcaa accatcatta aaataagagt atttgccatt gagtttgaaa
    15121 aattctctac aataaacttt taatcttcct ggaaccgtta agcgtttttt ggagttttct
    15181 attaatgatg ctaaaatctt ttgaggatgt ttttttgtaa tcacaatgtc ttccaactca
    15241 ttgacattgc tggttagata aatcgtatta actgtttctt ttaaagtatt cgatcggaga
    15301 tttatgggta aataagaaga atgttttatt tgaatattgg tgcttcctgt taactcaaaa
    15361 gttacaattc cattggcgtt actgagtaaa ttttgtttgg tttttaaaag agtaaccgta
    15421 acatcttcaa taggtgcatc ggtatcttta tcttttagta taatggtttt ggattcagtt
    15481 tgtgaaaaca attgaatact atagaaaaac aaaatcgcca ataaaccttt tacagacatt
    15541 ttacttgatt tttaacaaaa ctaataaaca ttctcaatca aatagaaaat tacactatga
    15601 attatcaaaa ggattattaa catttgagca aaagtgataa tcttgtaaac attcaataaa
    15661 aacctgtaag acatatagtt gtcattcatc ttaaatttgt actataacaa ttaaaacaaa
    15721 ttatcatgaa aaatttaata tcaattgcat ttttgctgct tgtaacattt agtcttcaag
    15781 cgcaaaacaa aaacgttttg aaagagactg aaactaaaac tactactgtg aaaaattcgg
    15841 aaggtgaaaa gaaaatcgtt actactaatg aaacggaaga agttcaaaac attcaacttg
    15901 aagaagccaa acccaacact ttgaacattc cgatgaaaga aagcccagta atggttacta
    15961 aaacaacgga gttaagtgtt gacgggaaaa caaaatatgt tgatgtagat cattctgctt
    16021 attatatgaa taatggtact aaataccaat ttaaaactga tgattacggt tattcgataa
    16081 tcaataataa ttctaaaaag aattcgtatt tacgaaaaac atcaaacaac aattatcttt
    16141 ttgtaaatga tggcaaagta tcctcagcct attttgatgc tgatggaaat ttaattattg
    16201 atacttataa tgagaaaaca gatagaatta ctaccgaaaa attcatggta gctaagtaat
    16261 aaataactct ctagccttaa aaacgaaaaa tccgaaagat aactttcgga ttttttatta
    16321 ctcgttatgt aaaaaggctt gcctatttaa aagtgtttct tcactttcta catgattatc
    16381 atcaggaata caacaatcaa caggacaaac agcagcacat tgtggttctt catgaaaacc
    16441 tttgcattca gtacactttc ctggaacaat ataatatata tcatcagaaa ttggagtttg
    16501 agcaatatcc gcatcaactt cagttccatc tggaagaata acttttcctt ttaactgtgt
    16561 tccatcttta tagcgccaat cgtctgcgcc ttcatatatt gctgtatttg gacattctgg
    16621 ttcacaagca ccacaattta tacagtcatc ggttattata attgccataa ttttatatta
    16681 gaaattggaa gtcagaagtt ggaagtattt ttgaaaaaca aaaccaacta atctaactat
    16741 attacttatt tttgtgcaaa attacaatca aaaccattca taaacaagaa acaaatgtta
    16801 caattggata aaaaagaatg ttttattgct ttaggaaaat ttctaagcca attttcaatg
    16861 gatgctaatt caaaaaatga aacagtactt tataacgatt tgttttacga tgattttatt
    16921 gatttaattc atttatccca atcgcataat ggttggttta caccagaaca agtttatttt
    16981 gctctaaatt cttgggcaga agctttaact gataaaaatt tatcgctttg gttgtctaaa
    17041 tattcctttg actgcgctca ggatgacaaa aagaaagtaa aaacagttgg attaatcttg
    17101 gcaggaaata ttcctttggt tggttttcat gattttcttt ctgtattgat ttcgggacat
    17161 aacgtcttgg taaaaacttc gtctaatgac caacatttaa tcaagttttt agcaaaatac
    17221 ctaattgctg taaatcctga aataaaaaat tcaatcactt ttacagacgg gaaacttgaa
    17281 gactttgatg ccgttatcgc tactggaagt aacaatactg cacgttattt tgagttttac
    17341 tttaaagaca aaccaagtat cattcgtaaa aacagaaatt caattgcagt tttagacgga
    17401 aatgaaacgc atgaagattt agttaattta ggtgaagaca tttttaggta ttttggctta
    17461 ggttgtcgca atgtttctaa attatttgtg ccaaaaggtt atgattttaa aaactttttt
    17521 gaagctattt ataaatataa agatgtaatt ttctatgaga aatattctaa taattacgac
    17581 tacaataaag ccgttttttt gatgagtaat tttaaattat tggataatga gtttctaact
    17641 ttaaaagaag atcctagtta tgcttcacca atttcttcgg tgttttatga aaattatgaa
    17701 gatttagaaa ctttaaaaag tcgtttagag cttgaaaaag agcaaattca gtgtatagtt
    17761 tctaatgatt taattccaaa atccatcgct tttggacaaa ctcaaaaacc acaactttgg
    17821 gattatgctg atagtgttga tactttggag tttttaataa atctttaaat ttctttttca
    17881 actataacgt ttttgttcat aacctttaac aattagttgt ctttccaatt gttaatttct
    17941 attgctattt ccgaaatttg caacttaatt ttttggaatc aacatgaaaa aacataatta
    18001 tagcgcagga ccatgtattt tacctcaaga agtatttgaa aaatcagccg aagcaatttt
    18061 aaattttaat aattctaatt tatccatcct cgaaatatcg cacagaagta aagactttgt
    18121 agcggtaatg gatgaggcaa gagcattagt tttggaactt ttaggattgg aaggaaaggg
    18181 ttatcaagca ttgtttcttc aaggtggcgc tagtatggag tttttaatgg ttccgtataa
    18241 tttgatgaat gttgatggaa aagcggcata tttagatact ggaacttggg caagcaatgc
    18301 tatcaaagaa gccaaacttt ttggaggaac agaaattgta gcatcgtcta aagaagctaa
    18361 ttataatttt attccaaagg aatatgctat tgcaaatgat gcttcttatt ttcattgcac
    18421 aagtaacaat acaatttttg gaacacaaat gaaatcattt ccacaaacca atgttccttt
    18481 agtttgtgat atgagttcgg atattttctc cagaacatta gatttttcaa aatttgattt
    18541 gatttatgct ggagcacaaa aaaatatggg accagctggt acaacacttg ttgttgtaaa
    18601 agaagctatt cttggtaaat caggcagaac tattccaagt attctagatt atgaaaaaca
    18661 tattaaagca gaaagtatgt ttaacacacc tgctgttttt ccagtgtacg cttcactcct
    18721 aactttacaa tggttaaaaa aacttggtgg aattgcagct attgaaaaaa taaacgaagc
    18781 caaagcaaat ttactttaca acgagataga tagaaatcca ttgtttaaag gtgcagcaaa
    18841 taaagaagat cgaagcatta tgaatgccac gtttctttta gaaaatgaag cacaccaaga
    18901 aacatttgat aaaatgtgta aagatgctgg aatttctgga attaatggtc atcgttccgt
    18961 tggtggttat cgtgcttcga tgtataatgc tttaccaatt gaaagtgtac aagtgctggt
    19021 agccgttatg caagaattag aaagaaatgt ttaataaaag tagaagaaaa aataattaca
    19081 gcatcgagag atgttgacat aaattcaaca tagaatgaaa atattagcaa acgatggaat
    19141 ttcaaaaagt ggtattaaag ccttagaaaa aggcggtttt gaagtaatca ctacaaaagt
    19201 agcgcaagaa caagttgcca attatattaa ttcaaataat gtatcggtta tattagttcg
    19261 cagtgctacc aaagtacggc aagatattat tgacaattgt ccgagtttaa aaataattgg
    19321 tcgcggtggt gttggaatgg acaatattga tgtagaatat gctcgcagca aaggaattca
    19381 tgtcattaac acaccagctt cttcatcaga aagtgttgct gaattggttt ttgcacattt
    19441 atttactggc gttcgttttc ttcatgatgc caacagaaat atgccattag aaggcgatag
    19501 caattttgat ggtttaaaaa aagcctatgc caatggtatt gaacttagag gaaaaacatt
    19561 agggattatt ggttttggaa aaattggtca agcggttgcc aaaatggctg ttggattggg
    19621 aatgaaagtg attgcatctg ataaatttgt tggtaaagca gatgttagag ttgatttcta
    19681 taacgggcaa ttcatcaatg tagagattga aaccgaacca ctagaggatt tattaagaca
    19741 ctctgatttt atcacacttc acgttcctgc acaagacacc tatgttattg acaaagatga
    19801 attggctatt atgaaagata atgtcggcat catcaactgt gctcgtggtg gtgttttaaa
    19861 tgaagttgct ttaattgaag ccttagaaaa taataaagtt ctatttgcag gattagatgt
    19921 ttttgaaaaa gaaccaagtc cagaaattca aatattaatg catcctagaa tatcattgac
    19981 accgcatatt ggtgccgcaa ctttagaagc acaggataga attggaactg agttagcaga
    20041 gcaaattatt agtatcctca aaacagagaa agtataactg taaacgaatt aacaaagata
    20101 actccaaaaa agttatcttt ttttttgtac atttaaggtc aattaaaaat tttaatatca
    20161 tgtttgaaca attatcagaa ttagtaaatc aattttgaaa tgatgcagta gtaaataaca
    20221 atgctgttcc aaacgaacaa aaccaagccg ttttaagtga agctaattct tctattatgg
    20281 atggtttaaa aggaatggtt gctaacggaa acgtttcgga tttagtaggt ttgtttcaag
    20341 gaaataccgc tattgatagt tcgaatcctg tggtacagca attgacacaa aaattttcag
    20401 gaaatttagg tcaaaagttt ggattatcta acgaagcttc ttctggagtg gcagctagtt
    20461 taattccaaa agttttaggt tcattgattg gaaatgcgaa agacccaaat cataagggtt
    20521 ttgaagtatc ggatattata aacgcaattt ctaatggaca aggaaactca ggtttgatgg
    20581 atgctatttc taattatggc ggacaacttg gtttagacca aaatggtgat ggaaaagttg
    20641 gtatggatga tgcaattgct gcagtttcta ataaatctgg tggtttaggc ggaatgttag
    20701 gcaaactttt cgggaagtaa aaaatataat tttcataaaa gccatgtgaa aacatggttt
    20761 ttcttttttt aacacattcc taatagaaaa caactcacta aattagtaac tttagtaaaa
    20821 attttgaaat gaaaaatcta attcctttta tagtcatcat tgctatagta attggttgca
    20881 acagttcaaa atcaggattt aatgctactg attctttggt tgcaaacgat actattaaaa
    20941 ttgaaaatga cagtttagaa taccaagtca ttattattga tactgggttt aattcttggt
    21001 tagtttcaac agcaaaacct cgtggctatt attctgaaag atatttagaa gatagaaatt
    21061 acatttatgt tcaagaatgg aacaatagag taaatcaacc gtcgcgatat ggaatagatt
    21121 tatacgaaat gcgtattgat tatgacagaa atattcatta tggttatgaa gtaaattatt
    21181 taatatacaa ctatatgatt tattttcaaa ttaagaacaa tcaacgtttg tcgggctttg
    21241 ttccaagacc ataaaaaagt atatttgcaa taaaaaaaga atggcttctt tcaaagaacg
    21301 ttggaacatt acatcaaaca cacaaatggt ggttatactt gttgtatttg ccattactgg
    21361 ttctagttcg gcactacttt caaaacccat tttggcagta tttggaatta ttaaaggaga
    21421 tgtttccaat tggatatatt atcctttata tattctttta attttcccag tttatcaagt
    21481 attgttaatc agttttggct ttctcttcgg acagtttaaa ttcttttggg cttttgagaa
    21541 aaaaatgctc aaaagtcttg ggtttggatt tttttttaaa aaagaaaatc tcgaataatg
    21601 caacttgata aactcagcaa cttaaccaat tcaataatta aaaccttagt tttgtttttg
    21661 tttacccact aaattaaaaa aatcgtttct attttctttt tcgttaaaaa ttccaccata
    21721 ctgtatggtt gaggtaaaac tactttcatc ttgaattcct ctactcgaca cacaaagatg
    21781 ttttgcttcc ataactacaa ttacatcatc agtgtctaaa acagttttca attcattaaa
    21841 aatttgcata atcaatcgtt cttgcacttg tggtcttcta gagtaataat ccactattct
    21901 attcaatttt gaaagaccaa ttacttttcc actagacaca tatccaatat gagcttttcc
    21961 aataattgga agaaaatgat gttcacaagt tgaattaaaa ctaatagctg cttcaaccaa
    22021 cattttatga tagtggtaag aattttcaaa aaccgaaatt tttggtttat tttctggatt
    22081 taatccggag aaaatctctt taataaacat tttagcaact cgatgtggag ttccttttaa
    22141 actatcatca gtcatgtcta agcccattac ttccataata gcatgaaaat gatcttcaat
    22201 aaccatcatt ttttcattat cgtttttgtg aaatgcatca gctctcaatg gtgtatttgc
    22261 tgaagtcata tgatgattgt cgcccatcaa ttcgattaat tccttttcaa tagtagtatt
    22321 catagaagtt tatttttgtt taacaaattt ataaaataat taaacaataa attttattta
    22381 acttaaattt ataatttatt caatcataaa aaaaacttat agttagtttt aaaaacaaaa
    22441 tactttgtta ctttgtaaaa aaaatagaga atgataactg ccaataatat atttaaaaat
    22501 tacgatgcac ttcaagtttt aaaaggagta acactacata tcgaaaaggg agaaatcgtt
    22561 tcgattgttg gtgctagtgg cgcaggaaaa actactttac tacaaattat tggaacttta
    22621 gaccatccta acacaaacaa agaaactact ctagagataa acggtaaaaa cattctaaaa
    22681 atggatgatg cgacattgtc taaatttaga aacgaacatt tagggtttat atttcagttt
    22741 caccaattac ttcctgaatt tactgcttta gaaaatgttt gtattccagc ttacattgcc
    22801 aaaaaggaaa aaacaccaac agaagctgaa gccaaaagac tcttagatta tctaggttta
    22861 tcgcacagga tacatcacaa accaaatgaa ctctcaggtg gagaacaaca acgtgttgct
    22921 gttgccagag ctttaatcaa caaaccagcc gtaatctttg ctgatgaacc ttcgggaaac
    22981 ttagactcta catcagctga aaatttacac gaattattct ttaaacttcg tgacgaattt
    23041 ggacaaacat tcgtaattgt tacccataat gaggctttag caaacatggc agaccgaaaa
    23101 ttaacaatgg ttgatggtaa tattgatact aatgtagatt ggattgactg gaaagccatt
    23161 aacaatattg aaacaaacta aagatgct
//
LOCUS       DAOOBH010000024        23132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_2896_length_23132_cov_5.502968, whole genome shotgun sequence.
ACCESSION   DAOOBH010000024 DAOOBH010000000
VERSION     DAOOBH010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 23132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23132
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2896_length_23132_cov_5.502968"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3641)
                     /locus_tag="PK218_04400"
     CDS             complement(<1..3641)
                     /locus_tag="PK218_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPW97779.1"
                     /translation="MKKIYSLILLLLSICSFGQAYDYTIYNTSNSGIASDYIGDIKVD
                     ANGLLWIASYSGVSTFNGTTFSNYNTSNSGIASNSIVKIEIDGSGKKWMATQSNGISS
                     LNGTTWTNYTTANSGLPNNAIDDIAIDGLNNVWIATSAGLTKFNGTTWTTYNSLNNIG
                     SVATDSNNGVWVTTGNILYKFNGTNFNSITDGVNKILKIKNNIIYLDAFDALMTYSTG
                     GTFIDIYFQSNSCLGGYQLNALDVNSNNKVWIAFNGSGLQNFTDCISYTSSNSGLPDN
                     YFSALRTQDSGTIWAGTLQLGLVKMTPTTSTCSAPTQTWSDNITATTATLGWIPAVPT
                     PDSYVIRYNTTNVIGGIQDATVSTSLSLENLTPNTDYHWWVASVCGEQQTNWVYGGFF
                     YTPPVTSNCNVPTNMMVYNLTSNSCVLGWTAPTPAPSSGYEVYLSTSNVAPVTNTTPT
                     YITNSTSITISNTLTPATSYYYWVRSNCGGTKSNWSPVLNFVTNGLSGCTTATYGQWP
                     ATNFTPACLGTTEQIVNNAYASEYTNVNIVANRQYTFSSSVLTDFITITNDSNVILAS
                     GTTPLNWSSQSTSGVIRYYLHTDSNCGAQNTNRIKYIQCVAPASCGLPTNLTVSNITS
                     NSCRLFWNAPTPVANSYDLYLNTTNTAPVTSTNATQTSNGNLIATLNGLSASTTYYYW
                     VRSNCNGTKSDWVSGGSFTTVAFLSCNGAVYGLYPDATFTPACTGNSEQIISNASAGE
                     YTNVNVLSNKQYTFTSSITTDYITITNATGTTVLASGLTPLVWNSSNTSGVIRYHFHA
                     NANCGVQNTSRIRSITCANASSCNPPTQFWSENITSSSATLNWIPAVPAPDGYIYVYN
                     TTNEIGGIDGSTTSTSASINDLQPNTDYHWWVASLCGNNQSEWVYGGLFTTLQSTTDC
                     FEKVAAGRTFTLAIKTDGTLWSWGNNDYGQLGDGTTTSRNTPSQVGTANDWASISCGV
                     NHAVAIKNNGTLWTWGFNIQGQLGDGTTVNKSVPTQIGTATNWEMVEAKNQHNIAKKT
                     NGTLWGWGSNFYGQLGDGTTVNKYTPTQIGTATNWQSVGTGGAHSMGVKTDGTLWCWG
                     LNTNGQVGDGTTTNRYIPTQIGTATNWRSVTGGSYFSIATRTNNTLWTWGRNNNGQLG
                     LNNTTDRTTPTQVGTATTWDKISAGEDHVLASRTYGAMYAWGYDLEGQIGNDESTNSV
                     LAPTLLLNVT"
     gene            complement(3696..4832)
                     /locus_tag="PK218_04405"
     CDS             complement(3696..4832)
                     /locus_tag="PK218_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97780.1"
                     /translation="MKADGTLWAWGVNSQGQLGLGTSTFSYHVPTQVGTDTNWQSISS
                     SDDYCLAIKTDGTLWSWGRNNFGQLGDNTTVNKNIPTQIGTDTNWQSVSAGHSYGLAI
                     KSNGTLWAWGNNGNGRLGDGTTTNRTVPTQIGTDANWQKVDGGYSHSLGIKTDGTLWA
                     WGTNTSGEFGIITSPLISYVPIQIGTDTNWQDVSASQWFSYAIKTNHSLWSTGRNNEG
                     QLGNGTSSTTNTLVFTQVGTFSDSYEIATGFYHVIEKNGDGFIRVTGKNNRGQLGNET
                     NVNINTLTYISCYPSTLSSENFEINELKAYPNPVSDVLHFSMDKEIERISICNLMGQE
                     IFKKETNATDVTIDVSNLTSGTYLAKITSNDANKTTKTIKIVKK"
     gene            complement(5225..6928)
                     /locus_tag="PK218_04410"
     CDS             complement(5225..6928)
                     /locus_tag="PK218_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW97781.1"
                     /translation="MIKFIYFLIPFTCFSSVFSQELNDFEVKRTRTIQEELISNPDKA
                     YADALEISNSKNELFGFFGKYYVANYFYNKSEFTHSKQLLVALIENIEKSKHAKSSKV
                     YQDLMGMCVNKLFYIHKNLGEYDTALFYLDTYKKSANNQFNEQYGLIKVAMGNYVDGI
                     AMLKKELKTTPHLKLGEGEKKVMNKKLFADKHNIIGEAYQKFYVQSRNTTLLDSANYY
                     FREAATMLIQENFQPEYTKALLYMREAKSAALEGNYNKALSLYRMRNKYPIIDENIRT
                     VQLFDLGMADCFHHLKQIDSALFYSKKYIISYESTKVSKENLLMAYTIMAQSYSEKKD
                     TKSAYLYAQKSLTLIQSIDGIKNKSVNFLHNYDLDSIRDESNSIIASKNYIKVSLLSI
                     LVVLVLVLSSFYFYYKRQKEKHHRFLKIIQNLKEFKTSSANPKTSSETIEPPKQLMDN
                     DLIEKLSLGLKKLEQKKTFLDPNFKLAFVAKKLNTNTAYLSQYFNQVMQKTFSEYTQE
                     LRIQYVLQKFNDVPQFRKYTLQAIAEEVGYKDATTFVRVFKKQTELSPNYYIEKLKNT
                     E"
     gene            7428..8342
                     /gene="cyoE"
                     /locus_tag="PK218_04415"
     CDS             7428..8342
                     /gene="cyoE"
                     /locus_tag="PK218_04415"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HPW97782.1"
                     /translation="MNSTLNVTSKPFTLKSVATDFKEITKAGLAVSVVFSSIAGYLLG
                     VTSFSNLNWLTLLMLAVGGYCMVGASNAYNQVIEKDLDALMDRTKNRPVASGRMSPNR
                     ALFVASILTIIGLVLLYLINPKSAMFGAISIFLYTSIYTPLKTYTPLSVFVGAFPGAI
                     PFMLGWVAATNDFGIEAGTLFLIQFFWQFPHFWAIGWFLYEDYEKAGFFMLPTGKKDT
                     STALQTVLYSVWLLIASLLPALGYTGRLFLTPIAAVFVAFIGLWMIYYAARLYKLRTS
                     KAAKQLMLVSVSYITLLQLIYIFDKFLR"
     gene            8348..8929
                     /locus_tag="PK218_04420"
     CDS             8348..8929
                     /locus_tag="PK218_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HPW97783.1"
                     /translation="MTMTAEEHKARSARSYKLLLLFAMLSMTMMFAGLTSAFVVSKSR
                     GDWLQNFEMPKAFFVSVVVILLCSVTFHLAKLSIKKDNRKATTNYLLATLVLGFLFVF
                     FQFQGFSEIVKQGYFFTGPESNITTTFLYVVTVVHLAHLFGGIISLLIIIYNHFKQKY
                     NSSQTLGIELGAMYWHFLDFLWVYLFLFLYFFK"
     gene            8974..10002
                     /locus_tag="PK218_04425"
     CDS             8974..10002
                     /locus_tag="PK218_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HPW97784.1"
                     /translation="MGATVTTANTNENTWGGGNEPMGASYGKMMMWFFIVSDALTFSG
                     FLAAYGFSRFKFIDNWPIADEVFTHFPFLHGVDAPMYYVALMTFILIFSSVTMVLAVD
                     AGHQMKQKKVAFYMLLTIVGGLIFVGSQAWEWKNFIKGEFGAVETKGGSVIQFVNKEG
                     KRVKIEDFAVVLPEEREELTRSKGKWFMSEATLPSYSVAEIQAGFKAHPEILVKTEKI
                     YRDTKEDQANKDLQPGLNHNKKREILTREASQKLVDNSHIVIEGANLKHNEYGNKLFA
                     DFFFFITGFHGFHVFSGVIINIIIFFNILLGTYEKRRSYEMVEKVGLYWHFVDLVWVF
                     VFTFFYLV"
     gene            10027..10392
                     /locus_tag="PK218_04430"
     CDS             10027..10392
                     /locus_tag="PK218_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HPW97785.1"
                     /translation="MSHDTHTHEHSSNIKRIWQVFGILSVVTIVEVYLGIIRPDALVM
                     NDFLAMNLLNWVFIILTLYKAYKITWVFMHMEGETASLRWAVVSTVIFLVLYLLFILL
                     VEADYIHDVFRDSTIKWNF"
     gene            10468..11124
                     /locus_tag="PK218_04435"
     CDS             10468..11124
                     /locus_tag="PK218_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97786.1"
                     /translation="MKKKTVLIVLFMLPLVVYLFFATGVNSFMRLPIVTPKVEDVNSN
                     WVALNGEKVTLNNKITILGFSGENMLDNRGNYFLLTDKIYSRNKAFKDFQIVFIAPEG
                     TQDQVKSVLERLSKLTNVSGYHFVFAPVAEIEAYYKQLKLVEKLDSHHGTKFVYIIDK
                     ERNLRGRKGKSVQGEAEYKEGYNINSSAELHNEMTDDVKIILAEYRLALKKNNAKRKI
                     "
     gene            11133..11825
                     /locus_tag="PK218_04440"
     CDS             11133..11825
                     /locus_tag="PK218_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HPW97787.1"
                     /translation="MMKNKSYIGVSLVILVFGIIFIPKIIERIKDGSVIQGDRIDGVS
                     NHRSKSANTGLVKIGPEPAFSLTNQDNKTITKKDYAGKVHVVEFFFSTCPSICPKMNQ
                     NMLVIEKEFLGNPNFGIISITINPDTDTPQILKKHAEALGVASSNWNFLTGKQDYIYD
                     LANKGFNLYAGQNSNVSGGFEHSGYFALVDKEGNIRSRKDKNGNPIIYYNGLEKEDVK
                     AIIKDIKKLLNE"
     gene            11818..12411
                     /locus_tag="PK218_04445"
     CDS             11818..12411
                     /locus_tag="PK218_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HPW97788.1"
                     /translation="MSKKEINSIDTKFNNLIMVVSILIPLIVAVLFSVKLKDFGIDVE
                     PLTFLPPIYATINGITALLLIAAVISIKNGKRKLHENLMTTAIMCSVVFLIMYVAYHL
                     TADSTKFGDVNHDGIVDEVELAKVGVWRIVYFLILISHIILSIVIIPLVLFTFARALA
                     ERFDKHKKLAKITFPIWLYVAITGVVVFIMISPYYAK"
     assembly_gap    12472..12571
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12637..12819
                     /locus_tag="PK218_04450"
     CDS             12637..12819
                     /locus_tag="PK218_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97789.1"
                     /translation="MFYTLNTTAQCAMCRASLESNGNNTQAEAVNDGIVYLMAIPYIL
                     VGVIGIFIYKMYFKKK"
     gene            complement(12946..14316)
                     /locus_tag="PK218_04455"
     CDS             complement(12946..14316)
                     /locus_tag="PK218_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HPW97790.1"
                     /translation="MSFHRITSLFIVVSFLMSSCGTTNKIEALKPLPSDDSPMVYKSN
                     TSFVSMPLEISLKEIEKQVNKKMTGLIYEDADMKIWKTADIKLTEKSGKIESILPLKI
                     WVKFKYGTNFMGLNDTREVNLNGTITLLSNARMTNFKLTTSSKIDDYVWSESPSIVVA
                     GRNVPITYIINPMLSMSKSKISKMIDDAINDLPDFKPVVLDVLENMSKPFMTSDVYQT
                     WFRLEPIEVYVTDAVLDKSKIAMDLGLKCNMLTMVGQEPKNIFDRTAVAFKPVTKVPD
                     KITANVAAISTYESASRIITKNFRGQEFASGSRKIVVNEVNLWQKDGKIIIQLLMSGS
                     LNGTIYLAGIPKYNAETHEIYFDQMDYVLQTKGMLTRTANWLLQGVILRKIQESCRYS
                     IKPNLDEGKKSMLPYLNNFSPMKGVFVNGTMNDFEFDRVELTNKAIIAFLKTTGKMNV
                     KIDGME"
     gene            14472..15797
                     /locus_tag="PK218_04460"
     CDS             14472..15797
                     /locus_tag="PK218_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW97791.1"
                     /translation="MKIIIKITFFFLLFATISIHAQQKKWTLEECIAYALENNISIQQ
                     SELDLKISDIEKLEAIGNFLPNISGTANYGINTGANINPTTNQFENQSFRSASANINT
                     GIAIFNGLANWKTLQRTKLNKIANTYRLDKMKDDIMLSIANAYLQILFNKEQIKVLSN
                     QNKITKENITRTKQLIDAGTLPAGDIYELQATDASQEQQIVNAENTLLISKLGLAQTL
                     LLKDYANFDIAVEDMDVPVTEILFQTPEAIAQKAREVVNDIKIASSNYEVAQKDVSIA
                     KSGYMPTLSGFLGYNTRWSETIPIGFVDQLSLFDGTNVGIQLNVPILNGFSTRGRVQR
                     SKVNEQRSKLLLDQAELDLERTVFQAYNDVLNAQKSYEAAVKTSEARKLAQNFSKERF
                     DVGLLNSFDYSQALITYENAQSEVIRTKYDYIFKIKLLEFYFGIPIIKQ"
     gene            15801..17096
                     /locus_tag="PK218_04465"
     CDS             15801..17096
                     /locus_tag="PK218_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW97792.1"
                     /translation="MSKKKIYWILAIVIVVIIALVVMKKKGMLGSNTELIEVESAAVN
                     EITIVETVSATGKIQPETEVKISSEVSGEIIALPIKEGQVVKKGDLLVKINPDLYTSG
                     YNRTISNLSGTKASLSQADASFKEAKASYDRSQTLFNKGIISKSDWDKAVAAFEVAKA
                     TKESAYYNVQSANATVKEAKDNLGRTTIYSPADGTISSLGVELGERVLGTQQMTGTEL
                     LRVADLNNMEVEVDVNENDIVKVSIGDSTKIQVDAYLKKDFKGIVTSISNSASNATTA
                     DQVTNFKVKIRILKESYQDLVEGKPATYSPFRPGMTATVDIITKRKEKVLGVPISSVV
                     VKSDTSATKKYEVKDVGDDKIKSKNDQKFECVFVKVGDKAKIKVIKTGIQDDTNIEVI
                     SGLQKGDVVITGPYTTVTKDLNSGDKVKSKTESATKAKK"
     gene            17096..17761
                     /gene="tsaB"
                     /locus_tag="PK218_04470"
     CDS             17096..17761
                     /gene="tsaB"
                     /locus_tag="PK218_04470"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPW97793.1"
                     /translation="MSLILNIETASKNCSVSLAKEGKTILCKEMSEEGYSHAEKLHVF
                     IEEILKETNTNFQELKAVAISKGPGSYTGLRIGVSAAKGICYALNIPLISVDTLQVLA
                     KQAKIDEGIIIPMLDARRMEVYSAVFDKNYNKIMDVQAEVVTAESYMEIEEDAYFVGD
                     CQEKCKTVLTKPNFHFVSNIIFPSANEMSALSHQKFVNQEWEDVAYFEPFYLKDFMIT
                     KKS"
     gene            complement(17764..18153)
                     /locus_tag="PK218_04475"
     CDS             complement(17764..18153)
                     /locus_tag="PK218_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW97794.1"
                     /translation="MFDKNNSVKIGSKLRKLRISKGYSQEYMAETLKISQKTYSNMEN
                     DKSSISIDTLKKIAQEYEIDLLDILSDEKVVVQNNNSHDASSFQGGIIFNQLSEELLN
                     QFKERIEDLKKVIEDKNKIIEILEKQL"
     gene            18558..18869
                     /locus_tag="PK218_04480"
     CDS             18558..18869
                     /locus_tag="PK218_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97795.1"
                     /translation="MGIDYDKVEIKNASEDGNGKVYGLFAYSKDELTKTAIVLDLINN
                     VFLISKAGATISCHSLDCTGTQCSALNSGQGWICTSCTKSCSKTTTVVIKAVDHSLEG
                     N"
     gene            18886..19422
                     /locus_tag="PK218_04485"
     CDS             18886..19422
                     /locus_tag="PK218_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97796.1"
                     /translation="MKTNCPFTHYLFFVFVLFCSCLSCKNSKEYVHSKKTFAKLKKDK
                     FEFTIDTLKFKNEISAGIFSEDSKIIFDKVEIIKQFTLGEKSEIFYYVLLTDFDKKVT
                     TAKWLYYDNGFLILNKKERLDDFFMIYLTCSGNQNCEPNVFIVDGKKKWVCGENSSCL
                     YNDGINKVECEKFTTVFQ"
     gene            19590..19838
                     /locus_tag="PK218_04490"
     CDS             19590..19838
                     /locus_tag="PK218_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97797.1"
                     /translation="MVLVLCVKNKLEEKITFKIIGKDLEDNEVKKDLVIQNDGKECFL
                     EIPKGIYTYEVILSNKETFKKGEYKFDDDITVTIKKEN"
     gene            complement(19868..20701)
                     /locus_tag="PK218_04495"
     CDS             complement(19868..20701)
                     /locus_tag="PK218_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPW97798.1"
                     /translation="MNKNNTITAFENYINKFFTVLIDYSPKLISAILILFIGLYAIRL
                     INRMVRKIMVKRDLDPTLSKFLADILLWTMRILLFVTFISKLGVETSSFVAILGAAGL
                     AVGLSLQGSLSNFAGGMLIILFKPFRVGHTIEAQGVIGTVCEIQIFVTKLVNANNQTI
                     FIPNGILSNDTIINYSMEGNRRADLTFSLSYDTNIKTAKDIVMNVLKNNPQVLQTPAP
                     AVITKNLTDSSIQLAIRPWAKLEDFFQMSSDVLEDCKEAFENAGIVIQPYVRELSVKM
                     D"
     gene            complement(20770..21198)
                     /locus_tag="PK218_04500"
     CDS             complement(20770..21198)
                     /locus_tag="PK218_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPW97799.1"
                     /translation="MSLLTNKKHIMGIGGFFKNLFGSSKEKVSEIADKAEDFAEGTFE
                     KVKETAAPIMEKVEEFAETAKEKVEEYMPQAKETVEKAYDSVKEKTSELADKAENFVE
                     NSVEKVKEKVNSFMNDASEDAEEIRTKAEDIVKPSDENAD"
     gene            complement(21335..21535)
                     /locus_tag="PK218_04505"
     CDS             complement(21335..21535)
                     /locus_tag="PK218_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPW97800.1"
                     /translation="MAVLKVIEVLSSSTVSWEDATQKAVAQASKSLKNIRSVYVQEQS
                     ATVKDGKVGEYRMNLKVTFELE"
     gene            complement(21597..22493)
                     /locus_tag="PK218_04510"
     CDS             complement(21597..22493)
                     /locus_tag="PK218_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPW97801.1"
                     /translation="MKITIKETQNPTILKFEFPDFITKNENFEFKNIDETKNSPLAQQ
                     LFYLPFVKTVYISGNFIAIERFSIVEWKDVQEDVAQQIENFISTGGKIILEDESKTKK
                     QPITVYGETTPNPAALKFVINKGITKSTVEFKNIDDAKASPLAQELFKLPYVKELFIT
                     ENYISVTKYESYTWEEITLELRTFIKQFIENGGTILDESLIEKSSKQDKQEIKNFDNL
                     DVTSQEIINILEEYVKPAVAADGGNILFDSYDETTKKVKVVLQGSCNGCPSSTFTLKN
                     GIENMLKSMLHNEDIVVEAING"
     gene            complement(22878..>23132)
                     /locus_tag="PK218_04515"
     CDS             complement(22878..>23132)
                     /locus_tag="PK218_04515"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPW97802.1"
                     /translation="CMEKPIDFQSMILNLHRFWSEKGCLIWQPYYTQVGAGTMNPATF
                     LRVLGPEPWRVAYVEPTIRPDDGRYGENPYRLQQHYQYLE"
BASE COUNT         7643 a         3772 c         3802 g         7815 t
ORIGIN      
        1 gtaacattta gcaataaagt aggagctaat acgctattag ttgattcatc atttccaatt
       61 tgaccttcta aatcatatcc ccatgcatac atagcgccat aggttctaga agctaaaaca
      121 tggtcttcgc cggctgatat tttatcccaa gtagttgctg ttccaacttg tgtaggggtt
      181 gttctatctg tagtattgtt taatcctaat tgaccattat tgtttctacc ccatgtccaa
      241 agcgtgttgt tggttcttgt agcaatacta aagtatgatc ctccagtaac acttctccag
      301 ttagttgccg tgccaatttg tgttggtata taacgatttg tggtagtgcc atcacctact
      361 tgtccattag tatttaatcc ccagcaccac aaagttccat cagtttttac acccatagaa
      421 tgcgctccgc cagtgcctac actttgccag ttagtagccg tgccaatttg tgttggggtg
      481 tatttattaa ctgtagttcc gtcacctaac tgtccataaa agttgcttcc ccaaccccat
      541 aaggtgccat tcgtcttttt ggcaatgttg tgttgatttt tagcttctac catttcccaa
      601 ttggtagcag ttcctatttg tgtaggaaca gatttgttta ccgttgttcc atcacccaat
      661 tgtccttgta tattgaatcc ccaagtccat aaagttccat tgttttttat cgcaacagca
      721 tgattgactc cacaagaaat actagcccaa tcatttgcag ttcctacttg cgatggtgtg
      781 tttctactag ttgttgttcc atctcccaac tgaccataat cattatttcc ccaagaccaa
      841 agtgtgccat ctgtttttat ggctaaggta aaagtacgtc ctgcagctac tttttcaaaa
      901 caatcggtag ttgattgtaa tgttgtaaat aagccaccat atacccattc agattggttg
      961 tttccacaaa gagaagcaac ccaccaatga taatcagtgt ttggttgtaa atcattaata
     1021 gaagcagatg ttgaagttgt gcttccatct atacctccaa tttcatttgt ggtattataa
     1081 acatagatgt aaccatctgg agctggaact gctggtatcc agtttagcgt tgctgaggat
     1141 gatgttatat tttcactcca aaattgtgtt ggcggattac aagaggaagc attcgcacac
     1201 gttatcgaac gaattctaga tgtattttga acgccacaat ttgcattggc atgaaaatgg
     1261 tatctaataa ctcctgatgt gttactagaa ttccaaacta atggcgttaa accactagcc
     1321 aaaaccgttg ttccagttgc atttgtaatg gtaatataat cagtagtaat agaactagta
     1381 aaagtatatt gcttatttga tagtacattt acattcgtat attctcctgc cgaagcattc
     1441 gaaataattt gttccgagtt tccagtacat gcaggtgtaa aagtcgcatc tggataaaga
     1501 ccataaactg caccattaca gcttaagaat gcaacagtcg taaaacttcc tccagaaacc
     1561 cagtcgcttt ttgttccatt acaattagaa cgtacccaat aatagtaagt tgttgaagct
     1621 gataaaccat ttaaagttgc tattaaatta ccattacttg tttgcgtggc attagttgaa
     1681 gttacaggag cagtattagt agtgtttaaa tataaatcat aagaattggc aacaggtgtt
     1741 ggtgcattcc aaaataatct acatgaatta gaagtgatgt ttgaaactgt caaattagtt
     1801 ggcaatccac agcttgctgg agccacacat tgaatatatt ttattctgtt tgtgttttgt
     1861 gcaccacaat tagaatcagt gtgtaaatag tagcgtatca ctccagatgt tgattgagaa
     1921 gaccaattta aaggtgttgt tccgcttgct agaataacat ttgaatcgtt cgttatcgtg
     1981 ataaaatccg ttaaaacaga acttgaaaac gtatattgtc tattggctac tatatttaca
     2041 ttggtatatt cactagcata agcattgtta acaatttgtt cggttgtacc caaacacgct
     2101 ggagtaaagt tagttgctgg ccattgtcca taggtagcag tagtacaacc cgataaacca
     2161 tttgtaacaa agtttagtac cggactccaa ttgctttttg tgccaccaca attagaacga
     2221 acccagtagt aataagatgt tgcaggtgtt aaggtattcg aaatggttat gctggtagaa
     2281 ttagttatat aggttggtgt tgtattggta actggagcaa catttgatgt agataaatac
     2341 acttcatagc cagagctagg tgctggtgtt ggtgctgtcc aaccaagtac gcacgaattt
     2401 gaagttaggt tataaaccat catattggtt ggaacattac aatttgaagt tactggcggt
     2461 gtataaaaga aacctccata gacccaattg gtttgttgtt cgccgcagac agaagcaacc
     2521 caccaatgat aatcagtgtt tggagttaaa ttctccaacg ataaagaggt agaaacagta
     2581 gcatcttgta ttcctcctat tacgtttgta gtgttgtaac gaatcacata actatcaggc
     2641 gttggaacag ctggtatcca acctagagtt gcagttgttg ctgtaatatt atcagaccaa
     2701 gtttgtgttg gcgcactaca tgttgaagta gttggtgtca ttttaaccaa accaagttgt
     2761 aacgttcctg cccaaatagt gcctgagtct tgtgttctaa gcgcactaaa ataattgtca
     2821 ggcaatcccg aattggaaga ggtataagag atacagtcgg taaagttttg taatccacta
     2881 ccgttgaatg caatccaaac tttgttgttg ctattcacgt caagcgcatt caactgatag
     2941 ccacctaggc aagagttgct ttgaaaataa atgtcaataa aagttccgcc agtagaataa
     3001 gtcattaatg cgtcaaaagc atcaagataa atgatgttat ttttgatttt caatatttta
     3061 tttacgccat ccgtaatact attgaaatta gttccgttga atttgtacaa aatatttcca
     3121 gtagttaccc aaacaccatt attactatcg gttgcaactg atccaatgtt gtttaaacta
     3181 ttgtaggttg tccaagtagt accattaaac tttgtaagtc cagcactcgt agcaatccaa
     3241 acgttattta atccgtctat ggcaatatcg tcaatcgcat tattaggaag tcctgagttt
     3301 gctgttgtat aattggtcca agttgttcca ttcaagctgc taattccgtt tgattgtgtt
     3361 gccatccatt ttttgcctga accatcaatt tcaattttta caattgaatt actagcaatg
     3421 cctgaattac tggtattgta gttggaaaaa gtagttccgt taaatgtgga aacaccactg
     3481 taagatgcta tccataataa tccgttggca tctactttaa tatcaccaat gtagtcactg
     3541 gcaatacccg aattggaagt gttataaata gtgtaatcat atgcttgtcc gaaagagcaa
     3601 atcgaaagaa gaagaagaat taatgagtaa atttttttca tgttttttga ttggtttaag
     3661 ttgcaatact ataaaaattg taggaattta taaaattatt tttttacaat tttgattgtt
     3721 ttcgttgttt tgtttgcatc attcgaagta atttttgcca aatatgttcc agatgttaaa
     3781 ttggaaacgt ctattgttac atccgttgca tttgtttctt ttttaaagat ttcttgtccc
     3841 attaaattac aaattgaaat cctttcaatt tctttatcca ttgaaaaatg taatacatca
     3901 ctaactgggt ttggatatgc ttttaattcg tttatttcaa aattttcaga agaaagtgtt
     3961 gaaggataac atgaaatgta ggtaagtgta tttatattta cattagtttc gttacctagt
     4021 tgacctctgt tattttttcc agtcactctt ataaagccat caccgttttt ttctattaca
     4081 tgataaaatc cagtagcaat ttcataagaa tctgaaaaag ttccaacttg agtaaaaaca
     4141 agcgtatttg ttgtgctact tgttccatta cctaactgtc cttcattgtt tctaccagta
     4201 ctccaaaggg aatggttggt tttaattgca taagaaaacc actgacttgc tgatacatct
     4261 tgccaattag tatcggtacc tatttgaata ggaacatagg atataagagg agatgttatt
     4321 atcccaaact caccacttgt gtttgtaccc catgcccaca gtgtaccatc tgtttttata
     4381 cccaaagaat gagaatagcc cccatctact ttttgccaat tagcatctgt tccaatttgt
     4441 gttggaacag ttctattggt ggttgttcca tcacctaatc ttccattacc attattaccc
     4501 catgcccaaa gtgttccatt ggattttatt gccaaaccat aactatgacc agctgaaaca
     4561 gattgccagt tggtatcagt tcctatttga gtaggtatgt ttttattcac ggttgtatta
     4621 tctcctaatt gcccgaaatt gtttcttccc caagaccata atgtaccatc tgtttttatt
     4681 gctaaacaat agtcgtccga ggaacttata ctttgccaat tagtatctgt tcctacttgt
     4741 gtaggtacgt ggtatgaaaa ggttgatgta cccaaaccca attggccttg agaatttaca
     4801 ccccaagccc aaagagtacc atctgcttta atagcatagg atgaactaaa attagcacaa
     4861 acttttagcc aattagcatc tgttccaatt tgtattggcg tatttttatc aactgtagtg
     4921 ttgtctccga gattacctac gccattagaa ccccatgccc aaagtgttcc atcttcttta
     4981 acagctaatg aatgactgta tccagcacta atggtttgtc agcattgtgc cgagatttgt
     5041 aatgttataa ataatactaa aatagtaatt gttcttttca tactttttgt tttgtggaaa
     5101 ttttattttg aagttaattt ttcaccaaaa ctaaaagata agagtaacta ctacaattta
     5161 tgtattgtac tatttataaa taatcagtag cctattaaga tttaattgtt ttatttttaa
     5221 ataattactc agtgtttttt aatttttcaa tataataatt tggagaaagt tctgtttgct
     5281 ttttaaaaac gcgaacaaac gtagtagcat ctttatatcc tacctcttcc gcaatagcct
     5341 gtaaagtata ttttcgaaat tgaggtacat cattaaactt ttgaagtaca tattgaattc
     5401 gtaattcttg tgtgtattcc gaaaaggttt tttgcattac ctgattgaag tattgtgaca
     5461 aataagccgt atttgtattt aattttttgg caacaaaagc caacttaaaa ttcggatcta
     5521 aaaaagtttt tttctgttct aattttttta accctaatga caatttctcg ataaggtcat
     5581 tgtccatgag ttgttttggt ggttctattg tttcactact tgttttagga ttagcactag
     5641 aagttttaaa ttcttttagg ttctgaataa tctttaaaaa acgatgatgc ttttcttttt
     5701 ggcgtttata gtaaaaataa aaactagaga gaactaaaac taaaactacc aaaatactta
     5761 aaagagaaac tttaatataa ttcttagagg caataattga attcgattca tctctaatgc
     5821 tatccaaatc gtaattatga agaaaattta cagatttatt ttttatacca tctattgatt
     5881 gaatcaaggt taaactcttt tgcgcataca gataagcact cttagtgtct tttttttcac
     5941 tgtaagattg tgccataata gtgtatgcca ttaatagatt ctctttagaa accttagttg
     6001 attcatagct tattatatat tttttactat aaaaaagtgc cgaatcaatt tgctttaaat
     6061 gatgaaaaca atcagccatt cctaaatcaa acaattgtac tgttcttata ttttcatcaa
     6121 taataggata cttatttctc attcgataaa gtgacaatgc tttgttgtaa tttccttcca
     6181 aagctgcgct ttttgcctca cgcatataca acaatgcttt tgtgtactcg ggttgaaaat
     6241 tttcttgtat cagcattgtt gcagcttctc taaagtaata attagccgaa tccagtaatg
     6301 ttgtatttct cgattgcaca taaaactttt gataagcttc gccaatgata ttgtgtttat
     6361 cggcaaataa ctttttattc attacctttt tttcgccttc tcctaatttt agatgtggtg
     6421 tggtttttaa ttctttcttc aacattgcaa taccgtcaac atagttaccc atagccactt
     6481 taatcaaacc gtattgttca ttaaattgat tgtttgcact ctttttgtat gtatcaagat
     6541 aaaacaatgc cgtatcatat tctcctaaat tcttgtggat gtaaaatagt ttgttgacac
     6601 acattcccat caaatcttga taaactttgc ttgatttggc atgcttgctt ttttcaatgt
     6661 tctcaataag tgctacaagg agttgttttg aatgagtaaa ctctgattta ttataaaagt
     6721 aattggccac ataatacttg ccaaaaaagc caaataactc gtttttagaa ttgctaattt
     6781 ctaaagcatc tgcataggct ttatcaggat ttgaaataag ttcttcttga atagttcggg
     6841 ttctttttac ctcaaaatca tttaattcct gagaaaacac agaagaaaaa catgtaaacg
     6901 gtattaaaaa atatataaat ttaatcatta tataaattga agcggaaaac tcgactaaaa
     6961 actattatgt acaaaaaaat aagctatttt acttgataca aacaataaaa gatagtcatc
     7021 aagcctaagc taacaacaaa aagcaaaaaa ttaaaaaaac aaatgtatta aagaattaca
     7081 gaatattttc tcatctataa agcaatttat aataatcaaa actaattttt aaattatcca
     7141 ataaattcta agtattcatg tattaaaatc caattaacat cttgcatttt tttttaaaaa
     7201 ttgctagact tgcaagtaac tgcgctaaat tcatcgaact acaactaaaa tgcaactgca
     7261 taaccaaaac aatcaaacgt acgattaaca tagttaaacg cacacctaaa acaatcaacc
     7321 gcacaacaac tacaatttaa cttccaaatt ccaccattat ttttatgaaa tgtttgtttg
     7381 tcaaatcact ttctataaag tacctttgca aaagtttttt aaaacaaatg aattcaacgt
     7441 tgaacgtcac ttcaaaacct tttacactca agtcagttgc tacggatttc aaagaaatca
     7501 ccaaagcagg tttggcagtt agtgtcgttt tctcttccat tgcaggatat ttattgggtg
     7561 ttaccagctt cagcaacctt aattggttaa ccttattaat gctagcagtt ggtggttatt
     7621 gcatggttgg agcatccaat gcctacaatc aagtcatcga aaaagacctc gacgctttga
     7681 tggacagaac caaaaatcgt ccagttgcct caggaagaat gtcaccaaat agagcgttat
     7741 ttgttgccag cattctgaca attattggtt tagtattgtt atacttaatc aatccaaaat
     7801 ccgccatgtt tggcgcgatt tccatatttt tatatacaag tatttataca cctttaaaaa
     7861 cctacacacc gctatccgtt tttgtaggtg catttccagg tgctattcca ttcatgttag
     7921 gttgggttgc cgccaccaat gattttggta tcgaagcagg aacattgttc ctaattcagt
     7981 ttttttggca attcccacat ttttgggcaa tcggttggtt tctttacgaa gactacgaga
     8041 aagcaggatt ttttatgttg cctaccggga aaaaagacac ctctacagct ttgcaaaccg
     8101 tgttgtattc tgtttggttg ctaatagcat cgcttttgcc agcattaggc tatacaggaa
     8161 gattatttct cacaccaata gcagcagttt ttgtagcttt cataggtttg tggatgatat
     8221 attacgcagc acgactatat aaactacgaa catctaaagc cgcaaaacaa ttgatgttag
     8281 taagcgtttc ctatattacc ttgttgcaat tgatttatat ttttgataaa tttttaagat
     8341 agaagatatg acaatgactg ccgaagaaca taaagcacga tcagcacgtt cctacaaact
     8401 gttgctgttg tttgctatgc taagcatgac catgatgttc gctggtttga ccagtgcttt
     8461 tgtagtaagt aaatcacgtg gcgattggtt gcaaaatttc gaaatgccta aagcgttttt
     8521 tgtgagcgta gtcgtgatat tactatgcag tgttacgttt catttagcaa aattgtctat
     8581 caaaaaagac aaccgaaaag ccactacaaa ttatctatta gcaactttag tattgggttt
     8641 tctgtttgtg tttttccaat ttcaagggtt tagtgaaata gtaaaacaag gctatttttt
     8701 cacaggacct gaaagtaata ttacaacaac ttttctctat gtagtaactg ttgttcactt
     8761 ggcgcatttg tttggtggaa ttatttcact tttaatcata atttataatc attttaaaca
     8821 aaaatacaat tcaagtcaaa cccttggaat tgaactaggt gcgatgtatt ggcactttct
     8881 tgatttctta tgggtttatt tgtttttatt tttatatttc tttaaataag aaaaaaaatc
     8941 taaatttggg aactttttaa cgaatcaact tttatgggag cgacagttac tactgcaaat
     9001 acaaatgaaa acacctgggg tggcggaaac gaaccaatgg gtgcaagtta tggcaaaatg
     9061 atgatgtggt tttttatcgt atcggatgct ttaacattct ctggattcct agctgcgtat
     9121 ggcttttcaa gatttaaatt tattgataat tggccaattg ccgacgaagt gtttactcac
     9181 tttccgtttt tacatggtgt agatgcgcca atgtactatg tggcattgat gacttttatc
     9241 ttgattttct cttcggtaac aatggtatta gccgttgatg ctggacatca aatgaaacaa
     9301 aaaaaggtag ctttttacat gcttttgact attgttggtg gtttaatctt cgtagggtca
     9361 caagcttggg aatggaaaaa cttcatcaaa ggagaatttg gagctgttga aaccaaaggc
     9421 ggaagtgtta ttcagtttgt aaacaaagaa ggtaaacgcg taaaaataga agattttgca
     9481 gtcgttttac cagaagaaag agaagaactt actagaagca aaggaaaatg gttcatgagc
     9541 gaggctacat tgccttcgta ttctgttgct gaaatacaag caggtttcaa agcacatcca
     9601 gaaatattgg taaaaacaga aaaaatttac agagatacta aagaagatca ggccaacaaa
     9661 gatttacagc ctggtttgaa tcataataag aaaagagaaa ttttgaccag agaagcttct
     9721 cagaaattag ttgataatag ccatatagtt attgaaggcg caaatcttaa gcacaacgag
     9781 tatggtaata agttatttgc cgatttcttt ttctttatca ctggttttca cggattccac
     9841 gtgttttctg gagtaatcat caatattatt attttcttta atattctatt aggaacctat
     9901 gagaaaagaa gaagctatga aatggtcgaa aaagttggac tatattggca ctttgtcgat
     9961 ttggtttggg tattcgtatt tacattcttc tatttagttt aatcatttac aataatacaa
    10021 attaatatgt cacacgatac tcacacacac gaacacagtt caaacatcaa aagaatttgg
    10081 caagtttttg gaattctttc tgtagttaca atagtagagg tttatctagg aattattagg
    10141 cctgatgctt tagtaatgaa tgatttttta gcaatgaatt tgcttaattg ggttttcatc
    10201 atcttaacac tttacaaagc ctataaaatt acatgggtat tcatgcacat ggaaggagaa
    10261 accgcaagtt tacgttgggc tgttgtatca accgttattt tcttagtatt gtacttatta
    10321 ttcattctat tagttgaggc agattacatt cacgatgtct ttagagattc tactatcaaa
    10381 tggaattttt aacaaaatat taattcaata aaaagggtac gctttgcgta ccttttttta
    10441 tttttgcact accaatttta tattgaaatg aaaaagaaaa ccgttctgat tgtacttttt
    10501 atgcttccac tagttgttta cctctttttt gcaacaggcg ttaatagttt catgaggttg
    10561 cctatagtta ctccaaaagt tgaagacgtc aattccaatt gggtagctct caatggcgaa
    10621 aaagtcacac ttaataataa aatcaccata ctagggtttt ctggcgaaaa catgttagat
    10681 aatcggggaa attatttttt attaaccgat aaaatataca gtagaaataa agccttcaaa
    10741 gattttcaaa ttgtcttcat cgctccagaa ggaacacaag accaagtaaa gtcagtttta
    10801 gaacggttaa gcaaacttac caatgtttct gggtatcatt ttgtttttgc accagtagcc
    10861 gaaatagaag cttattataa acaactgaag ctggttgaaa aactagacag tcatcatgga
    10921 actaaattcg tttatattat tgataaagaa cgcaacctaa gaggaagaaa aggcaaaagt
    10981 gttcaaggag aagccgaata taaagaagga tataacatca attcttcggc cgaattgcac
    11041 aatgaaatga ccgatgatgt aaaaattatt ttagcagaat accgtttagc attaaagaaa
    11101 aataatgcta agcgaaaaat ataagtgtat aaatcatgaa aaataaatca tacataggcg
    11161 tttcattagt cattttggta tttggaatta tttttatccc aaaaattata gaacgtataa
    11221 aagacggttc cgtgatacaa ggagacagaa ttgatggcgt atccaatcac agaagcaaaa
    11281 gtgcaaatac cggtttggta aaaattggtc cagaacctgc tttttcacta accaatcaag
    11341 ataataaaac aattaccaaa aaagattatg caggaaaagt acatgtggta gagtttttct
    11401 tttccacttg tccatctatt tgtccaaaga tgaatcaaaa tatgttggtt atcgaaaaag
    11461 aattcttggg taatccaaac ttcggaataa tctctattac catcaatcca gatactgata
    11521 caccacaaat tttaaaaaaa catgcagaag cattaggagt tgcctcctca aactggaatt
    11581 tcctaaccgg taagcaagat tatatctatg atttggcgaa caaagggttc aatctctatg
    11641 ctggtcaaaa tagtaatgtg agtggtggct ttgaacattc aggctatttt gctttggtag
    11701 ataaagaagg caacatcaga agccgaaaag acaagaatgg caatccaatt atttattaca
    11761 acggattgga gaaagaagat gtaaaagcga ttataaaaga cataaaaaaa ttgttaaatg
    11821 agtaagaaag aaatcaactc cattgataca aaattcaaca acctaattat ggttgtttcg
    11881 atattaattc cgctaatagt tgcagtattg tttagtgtca aactaaaaga ttttgggata
    11941 gatgttgaac cgttaacttt tttaccaccc atttatgcta ctattaatgg gataacggct
    12001 ttactattaa ttgcagctgt aatttctatc aaaaatggca aacgaaaact tcacgaaaat
    12061 ttaatgacca cagccataat gtgttctgtt gtttttctaa tcatgtatgt agcctatcat
    12121 ctaacagccg attctaccaa gtttggtgat gttaatcacg atggaattgt ggatgaagta
    12181 gagcttgcaa aagttggtgt ttggcgcata gtttactttc taattctaat cagccacatc
    12241 attttatcca ttgtcattat tccgctagtg ttgttcacct ttgcacgagc attagccgaa
    12301 cgttttgata aacataaaaa attagccaaa atcacatttc caatttggtt atacgtagcc
    12361 attacaggcg ttgtagtatt cataatgatt tcgccctatt atgcaaaata aagaacaaag
    12421 aacaaagcaa aaagaataca gattgctatc cataaagagt ggcttctttc tnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaagaacaa agcaaaaaga atacagattg
    12601 ctatccataa agagtggctt ctttcttctg tgttctatgt tttatactct aaacacaacc
    12661 gcccaatgtg ccatgtgtcg cgcatcacta gaaagcaatg gcaacaatac ccaagccgaa
    12721 gctgtaaacg acggaatcgt ttacctaatg gcaattcctt atatattagt gggtgtcatc
    12781 ggaatattta tttataaaat gtattttaag aaaaagtaat ttctttaaca ctttttttaa
    12841 aaaaccttta gaaacttatt tgtagccttt agcagttcat ttgtagcttt tagcaattag
    12901 tttatagctt ttagcaattc atttcaaggt tttagcagca actatttatt ccatcccatc
    12961 tattttcaca ttcatttttc cagtagtttt caaaaaagca atgatggctt tattggtaag
    13021 ttctactcta tcaaattcaa aatcattcat cgtgccgtta acaaaaacac ctttcattgg
    13081 tgaaaaatta ttcaaatagg gcaacatgct tttcttgcct tcatcaagat taggcttaat
    13141 agaataacgg caactctctt gtatttttct caaaataact ccctgcaaca accaattagc
    13201 cgttcgagtt agcatgcctt ttgtttgcaa aacataatcc atttggtcaa aataaatctc
    13261 atgagtttca gcattatatt taggaattcc cgccaaatat atcgtgccgt tcaaactgcc
    13321 actcatcaat aattgaataa taatcttccc gtctttttgc caaagattta cttcattcac
    13381 cacaattttt ctactgccag atgcaaactc ttgtccacga aagtttttag taattatcct
    13441 cgaagcactt tcataagtag aaatagcagc aacattggca gtaatcttgt cgggaacttt
    13501 cgttacaggt ttaaaggcaa ccgcagttcg gtcaaaaata ttctttggct cttgcccaac
    13561 cattgttagc atgttgcatt tcaaacccaa atccatagcg attttagatt tatccaaaac
    13621 agcatccgtc acataaactt caataggttc cagtcgaaac catgtttgat acacgtcgct
    13681 tgtcataaat ggtttgctca tattttctaa aacatccaaa acaacaggtt taaaatcggg
    13741 caaatcatta atagcatcat caatcatttt agaaatttta gatttcgaca tcgataacat
    13801 agggttaata atataggtaa tcggaacatt tcttcctgcg accactatcg atgggctttc
    13861 actccacaca tagtcatcaa ttttagaact cgtcgtcaac ttaaaattgg tcattctagc
    13921 attactcaaa agcgtaatcg taccattcaa attcacttct ctcgtatcat tcaagcccat
    13981 aaaattagtg ccatatttga attttaccca aattttcagt ggcaaaatag attcaatttt
    14041 tccgcttttt tcagtcagtt taatgtcagc tgttttccaa atcttcatgt cagcatcttc
    14101 ataaatcaaa cctgtcattt ttttgttcac ctgcttttca atctctttca aagaaatttc
    14161 caaaggcatg ctcacaaaag aagtattcga cttgtaaacc atcggagaat catcggaagg
    14221 taaaggtttt aaagcttcaa ttttgtttgt ggtgccacaa cttgacatca aaaaagaaac
    14281 aacaataaat aaagaagtta tacgatgaaa tgacatattg gattgatttt tttggcaaaa
    14341 atagacgtaa aacagaaata aagtgtaaca atttcaaaaa actaaagtct aaatgggtaa
    14401 gtcattacta tttcatgtaa ttcaaaagtt ttatctttgt tgcgcaaaga atggtagtat
    14461 tattattaac tatgaaaatc atcatcaaaa tcacattttt ctttttattg ttcgctacta
    14521 tttcgataca tgcgcaacaa aagaaatgga ccttagaaga atgtattgcc tacgctttag
    14581 aaaacaatat ttctattcaa caatcggaat tagatttgaa gatttcagat attgaaaagt
    14641 tggaagctat tggaaatttt cttccaaata tatcaggaac ggcaaattat ggtatcaata
    14701 caggtgcaaa tattaaccca acaaccaatc agttcgaaaa tcaatcgttc cgttcggctt
    14761 cagcaaatat taatactgga attgctattt ttaatggatt ggccaactgg aaaacacttc
    14821 aaagaaccaa gcttaataaa atagcaaata cctatcgttt agacaaaatg aaagacgata
    14881 tcatgcttag tattgccaat gcctatttgc aaattttatt caataaagaa caaataaaag
    14941 tattatcaaa tcaaaataaa atcaccaaag aaaacatcac aagaacaaaa caattgattg
    15001 atgctggaac acttcctgca ggtgatattt acgaattaca agctaccgat gcttcacaag
    15061 aacaacaaat tgtaaatgct gaaaatactt tattaatttc aaaattaggt ttggcgcaaa
    15121 cgcttttact aaaagattat gccaattttg atattgcagt agaggatatg gacgttcctg
    15181 taaccgaaat actttttcaa acacccgaag ctattgctca aaaagccaga gaagtagtta
    15241 atgatatcaa aattgcttct tcaaattatg aagttgcaca aaaagatgtt tcaatagcaa
    15301 aatcagggta tatgccaacc ctttcaggct ttttgggata caatacacgc tggtcagaaa
    15361 ctattccaat tggttttgtt gaccagttat ctctttttga tggaactaat gttggtattc
    15421 aactcaatgt tcccatatta aatggttttt caactcgggg aagagtacaa cgaagtaaag
    15481 tcaacgaaca acgttcaaaa ttactactag accaagccga attagattta gaacgaaccg
    15541 tttttcaagc atacaatgat gtgttgaatg cacaaaaaag ttatgaagcc gcagtgaaaa
    15601 cttcagaagc cagaaagtta gcacaaaatt tctctaaaga acgtttcgat gttggacttt
    15661 taaattcgtt tgattacagt caagcgttaa tcacttatga aaatgcacaa tcggaagtaa
    15721 tacgaacaaa atacgattat atttttaaaa ttaaattatt agaattttat ttcggaatcc
    15781 caatcattaa acaataaatc atgtcaaaga aaaaaatata ctggatactt gcaattgtaa
    15841 tagttgtcat cattgcttta gttgtaatga agaaaaaagg aatgcttggt tccaatactg
    15901 aattaataga ggtagaatcg gcagcagtaa atgaaattac tatagttgaa accgtttcag
    15961 caaccgggaa aattcaaccc gaaaccgaag taaaaatatc atctgaagtt tcaggagaaa
    16021 tcattgcttt gccaataaaa gaaggtcaag tagtcaaaaa aggcgactta ttagtaaaaa
    16081 taaatcccga tttatatact tcgggttata atagaacgat ttcaaatcta tcaggaacaa
    16141 aggcaagttt aagtcaagcc gacgcttcat tcaaagaagc caaagcaagt tatgacagaa
    16201 gccaaacgtt attcaacaaa ggaattattt caaaatcaga ttgggataaa gcagttgcag
    16261 catttgaagt tgcaaaagct accaaagaat cggcttatta caatgttcaa agcgccaatg
    16321 caacggttaa agaagccaaa gacaacttgg gtagaactac gatttattcg ccagcagatg
    16381 gaaccatttc ttctctagga gtagagcttg gagaacgagt tcttggaaca caacaaatga
    16441 caggaactga acttttgcga gtagccgatt taaacaatat ggaagttgaa gttgatgtaa
    16501 atgaaaatga tattgtaaaa gtttctattg gagacagcac caaaatacaa gtagatgctt
    16561 atttgaagaa agactttaaa ggaatcgtta ctagtatttc aaactcggcg agtaatgcaa
    16621 caacggcaga ccaagtaacc aattttaaag taaaaattag aattctaaaa gaatcctatc
    16681 aagacctagt tgaaggaaaa ccagcgacat attctccatt tagaccagga atgacagcaa
    16741 cggttgatat tatcaccaaa agaaaagaaa aagtattggg tgtgcctatt agttcagtag
    16801 ttgtaaaatc agatacttcg gctactaaga aatatgaagt aaaagacgtt ggtgatgata
    16861 aaataaaatc aaaaaacgat caaaaatttg aatgtgtttt tgtaaaagta ggagataagg
    16921 caaaaataaa agtaatcaaa acaggtattc aagacgatac taacattgaa gtgatttcgg
    16981 gcttacaaaa aggagatgta gtcatcaccg gaccttatac cacggttacc aaagacttaa
    17041 attctggcga taaagtaaag tcaaaaacag aatcagccac aaaagcaaaa aaataatgtc
    17101 tttgatactt aatattgaaa cagcctccaa aaactgttcg gtttctttag caaaagaagg
    17161 aaaaacaatt ttatgcaaag aaatgtctga ggaaggctat tcacatgcag agaaattgca
    17221 tgtttttatt gaagagattt taaaagaaac caatacaaat tttcaagaat tgaaagccgt
    17281 tgccataagt aaaggtccag gttcttatac cggattgcgt attggagttt cggcagcaaa
    17341 aggaatttgt tatgctttaa atattccatt aatttcagtg gatactttac aggttttagc
    17401 caaacaagcc aaaattgatg aaggaatcat catcccgatg cttgatgctc gtagaatgga
    17461 agtgtatagt gctgtttttg ataaaaatta caataaaata atggatgttc aagcagaagt
    17521 agtaactgct gaaagttata tggaaattga agaagatgcc tattttgttg gtgattgtca
    17581 agagaaatgt aaaaccgttt taaccaagcc taattttcat tttgtatcga atattatttt
    17641 cccatcagca aatgaaatga gtgctttgag ccatcaaaaa tttgtaaacc aagaatggga
    17701 agatgtggct tactttgagc cgttttatct aaaagatttt atgattacta agaaaagtta
    17761 attttataat tgtttttcta atatttcaat tattttgttt ttatcctcaa tcactttttt
    17821 taagtcttca attctttctt tgaattgatt aagtaattct tctgatagtt ggttgaatat
    17881 tattccacct tgaaaagaac tcgcatcatg agaattgttg ttttgtacta caactttttc
    17941 atcagataaa atatctaaca aatctatttc atactcttga gctattttct ttaatgtatc
    18001 aattgatatt gaagacttat cattttccat attggaatat gttttttgag atatttttaa
    18061 tgtttcagcc atatattctt gggaataccc ttttgaaatt cttaactttc ttaatttact
    18121 acctattttt acagagttgt ttttatcaaa catgactatc gaattacaaa attaaaccat
    18181 ctataaggaa taaaaaaaag taacgtgtac taacattact aatttttata atttttttgt
    18241 caatgtaagt aataacttac taatgaaatc atggttaacc aattaatttt acaaaataat
    18301 ttttcgcgaa aattattgta ttatttaatt taatgtttaa ttttagaatt attaaaaata
    18361 aaaaaattat tatttggcag ttttgccttg tttatgttta ttaacttatc aattgctcaa
    18421 aaaaatgaag ttatatattc aaattcaaaa cttagaacag gggataaagt aggggttgtt
    18481 caagatggag tttttattac aactatagaa aaaagtaaat tattagaaga atttgctata
    18541 atagcaaaag actctggatg ggtattgatt atgataaagt agaaataaaa aatgcttccg
    18601 aagatggaaa tggaaaggtt tatggacttt ttgcatatag taaagatgaa ttaacaaaaa
    18661 ctgcaattgt tttagatttg attaataatg tctttcttat ttcaaaagca ggagcaacaa
    18721 tatcttgtca tagtttagat tgtactggaa cccaatgtag tgcattaaac tcaggacaag
    18781 gttggatttg cacttcttgt actaaatctt gttcaaaaac tacaacggtt gtaataaaag
    18841 cagttgacca ctctttagaa gggaattaat tttaaacgaa taaatatgaa gacaaattgt
    18901 ccttttactc attatctgtt ttttgttttt gtattgtttt gttcatgttt gtcttgtaaa
    18961 aatagtaaag aatatgttca tagtaaaaaa acttttgcta aattaaaaaa agacaaattt
    19021 gaatttacaa tagatacatt aaagtttaaa aatgaaattt ctgctgggat tttttcagaa
    19081 gattccaaga taatttttga taaggttgaa attataaaac aatttacgct tggtgaaaaa
    19141 agtgaaatat tctattatgt tttattaaca gattttgaca aaaaagtaac cactgcaaaa
    19201 tggctttact atgataacgg ttttttgata ctgaataaaa aagaaaggtt ggatgatttt
    19261 ttcatgattt atttaacttg ttcaggaaat caaaattgtg aaccaaatgt atttatagtt
    19321 gatggaaaaa agaaatgggt ttgtggcgaa aattcaagtt gtttatataa tgatggtata
    19381 aataaggttg agtgtgaaaa attcacaact gtttttcagt aacttttaaa ataaattttc
    19441 tatgtcaaaa tatattttta tttttttcat aattccttta ttctcttttt tttcaatcag
    19501 actttgaaaa aacaattcaa gcaggaggaa taattatcaa cggattaagt tttttaaaaa
    19561 ataataaacc aagtcaaaat acaaataaaa tggttttggt gctttgtgta aagaataagc
    19621 tcgaagaaaa aatcactttt aaaattatcg gtaaagattt agaagacaat gaggttaaaa
    19681 aggatttagt tattcaaaat gatggaaaag aatgtttttt agaaatccct aaaggcattt
    19741 atacatatga agtcatactt tctaacaagg aaacttttaa aaaaggagag tataaattcg
    19801 atgatgatat tactgtaact attaaaaaag aaaattaaaa gcaagacctc atatttgaag
    19861 tcttttttta atccatcttt accgataact cacgaacata aggctgaatc acaattccag
    19921 cattttcaaa agcttctttg caatcttcta aaacatccga tgacatttgg aaaaaatctt
    19981 ctaattttgc ccaaggacga attgccaatt gaatggatga atccgttaaa tttttggtga
    20041 ttactgcggg tgctggagtt tgtaaaactt gaggattgtt tttcaaaaca ttcattacga
    20101 tgtcttttgc agttttaata ttggtgtcat acgaaagcga aaatgttaaa tctgcacggc
    20161 gattgccttc cattgaataa ttgatgatcg tatcgttaga taaaattcca tttgggataa
    20221 aaatagtttg gttgttggca tttaccaatt tggtcacaaa tatttgaatt tcacaaaccg
    20281 tcccaattac gccttgtgct tcaatggtat gtccaactcg aaagggttta aataaaataa
    20341 tcaacatacc gccagcaaaa ttagatagcg aaccttgtaa cgacaatcca acagccaaac
    20401 cagctgctcc aagaattgct acaaacgatg aggtttctac gccaagtttt gaaataaagg
    20461 ttacaaacaa taaaatacgc atcgtccaaa gcaaaatatc cgctaaaaat tttgataatg
    20521 ttgggtctaa atcacgtttt accataattt ttctaaccat tctgttgatt aatcgaatgg
    20581 cataaagacc aataaataaa atcaatatag ccgaaattaa ttttggagaa taatcgatta
    20641 aaaccgtgaa aaatttatta atgtaattct cgaaagctgt aattgtgttg ttcttgttca
    20701 tgatttttaa ttataaaaaa tcccgacaaa tcgggatagt ttagtagtac aaagatacaa
    20761 atcttaaatt taatcggcat tttcatccga aggtttaact atatcttcag cttttgttct
    20821 aatttcttcg gcatcttctg aggcatcatt cataaaagaa tttacttttt ctttaacctt
    20881 ttctactgaa ttttctacaa aattctcggc tttgtctgca agttcactag ttttttcttt
    20941 aacagaatca tatgcttttt ctacagtttc tttggcttgt ggcatatatt cttccacttt
    21001 ttctttggca gtttcagcaa attcttctac tttctccata attggtgcag cagtttcttt
    21061 tactttttca aaagttcctt cagcaaaatc ttctgcttta tccgctattt cgcttacttt
    21121 ttctttggat gaaccaaaca agtttttaaa aaatcctcca attcccatga tgtgtttttt
    21181 attggttagt aaagacaagt ttacgaaaaa taatcagtta attttcaatt gtaatattat
    21241 ttcttcaata gtgtttgtta gtttttttaa gacgatttta tttgatataa aaaaacgccc
    21301 aaaaatggac gtttgtaatt tgattttttg aagtttattc taattcaaat gtaactttta
    21361 aattcattct atattcgcca actttgccgt ctttcactgt cgcactttgc tcttgaacat
    21421 atacagaacg aatgtttttt aaactttttg aagcttgagc tactgctttt tgtgtggcgt
    21481 cttcccagct tactgttgaa cttgataata cttcgataac ttttaatact gccattttat
    21541 cttaaattta aagtgaattc taaatgtaca aaaaaatgct gatttattaa aaattattaa
    21601 ccattaattg cttcaacaac aatgtcttca ttgtgtaaca tgctttttaa catgttttca
    21661 attccatttt ttaaagtaaa tgttgatgac ggacaaccat tgcacgaacc ttggagtact
    21721 actttcactt tcttagtagt ttcatcataa gaatcaaata aaatatttcc accatcagca
    21781 gctactgcgg gtttcacata ttcttctagt atgttgatga tttcttgtga agtgacatca
    21841 agattgtcaa aatttttgat ttcttgtttg tcttgttttg aagacttttc aattaaactt
    21901 tcgtcaagaa tagttccacc attttcaata aattgtttga taaaagttcg taattctaaa
    21961 gtgatttctt cccaagtata actttcgtat ttagtaacag aaatataatt ttcggtgatg
    22021 aataattctt tcacataagg cagtttgaat aattcttgcg ctagtggaga tgctttggca
    22081 tcatcaatat tcttaaattc aacagttgat ttggtgattc ctttattaat cacaaatttt
    22141 agcgctgctg gatttggagt cgtttcacca tatactgtaa taggttgctt tttagttttg
    22201 ctttcgtctt caagaataat tttcccacca gtagaaatga aattttctat ttgttgggca
    22261 acatcttctt gaacatcttt ccattcaacg atgctaaaac gttcaatggc tatgaaattc
    22321 cctgaaatat aaaccgtttt aacaaaagga agataaaata gttgttgtgc caaaggtgaa
    22381 tttttggttt catcaatgtt tttgaattca aagttttcgt ttttagtgat aaaatctggg
    22441 aattcaaatt taagtatagt tgggttttgg gtttctttta ttgtgatttt cattagtcta
    22501 gtgtattttt ctgcaaattt aacaaagtta ttttcaatga ttggttatat ttggagattt
    22561 taataaacat taacaatttg atttaagttt aaaattgttg cttttttaaa tacatatgaa
    22621 acaaaaatta ctatacctac ttttcttttt tggattattt caagcgttag ctcagcctat
    22681 aactgttaat accacaactt atactgtgcc gcaattggtg caagatgttt tgtttggtaa
    22741 tggcgcggct ggttcatctt gtgttggtac tttaaacaat ataacttgga gtactggaac
    22801 aaattttagc ggaggaaatc caaatgggat tggttatttt caaaacacaa accctacttt
    22861 tccacttacg aatggggtta ttctaagtac tggtaatgtt gctgcaggcg gtacgggttt
    22921 tcgccataac ggccatcatc cggccggatg gtcggctcga cataggccac gcgccagggt
    22981 tcagggccaa ggactcgaag gaaggtggcc gggttcatcg tgccggcacc aacctgggtg
    23041 taatatggct gccagatcag gcagcctttc tcagaccaaa atcggtgtaa gtttaaaatc
    23101 atggattgaa aatcaatcgg tttttccatg ca
//
LOCUS       DAOOBH010000025        21414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_3255_length_21414_cov_6.004963, whole genome shotgun sequence.
ACCESSION   DAOOBH010000025 DAOOBH010000000
VERSION     DAOOBH010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 21414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21414
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3255_length_21414_cov_6.004963"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1544
                     /locus_tag="PK218_04520"
     CDS             144..1544
                     /locus_tag="PK218_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW97803.1"
                     /translation="MHFDHEEEDRNLSLSKFESMLKTNKVFFFDSEEFEEIILHYLDM
                     GKATLAKKALKLGLEQHPKSTGLKLVQVEMLIYDEKLDQAEKMLNELYALEPTNEEIY
                     ILKANIYSKKDKHETAVELLETALQYSEDHADIYNLVGMEYLFMDNLEMAKINFIKCL
                     EEDFEDQSGLYNVVYCFEFLDQNQEAIDYLKTYIEKNPYSEIAWHQTGRLYYGLKNYE
                     LAATSFELATYIDDEFIGAFLERGKALERLKRYDEAIVSYNQTIVLDDPTSYALLRIG
                     KCYEKLGNKVEALKYYNKTVHEDPLLDKGWIAITDFYVSQKNHQKALYYVNKALAIDD
                     QNRLYWKRFATINKAMNNIEEAEYGYRKAVELGDFQLDTWLFWVDIMKIMGEHNNGIL
                     TLIQAAEYFPDQFEIEYRMAGFHFLLNEEEKGNFHLNNALRLNSKHVSILEELFPLVW
                     DRVSVQEYISKHKKSE"
     gene            1545..2285
                     /locus_tag="PK218_04525"
     CDS             1545..2285
                     /locus_tag="PK218_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPW97804.1"
                     /translation="MSKRQFGLIGKNISYSFSKKYFTEKFALGNLEDYFYENFDLQSI
                     DEFPEILKNNSDLKGLNVTIPFKETIIPYLDKLSKNAQKIGAVNVIRFTKKGSLKGYN
                     SDYYGFIKSLKPLLKEHHKKALILGTGGASKGIAFGLEKLGILYTFVSREEKEGMINY
                     NRINATTFDNYKIIINCTPLGTSPNVKEFPNLPYQFFTKKHIAFDLIYNPEETQFLKK
                     AKKKGATIKNGFEMLQYQAEKSWKIWNK"
     gene            2285..2758
                     /gene="rlmH"
                     /locus_tag="PK218_04530"
     CDS             2285..2758
                     /gene="rlmH"
                     /locus_tag="PK218_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPW97805.1"
                     /translation="MNIKLLAIGKTDNKSLQTLIDDYTKRLSFYIKFDLEIIPDIKNV
                     KNLSEAQQKEKEGELILTKLTPTDQLILLDENGTTFSSVGFSEYLQKKMNSGVKTLVF
                     VIGGPYGFSDEVYRKAQGKISLSEMTFSHQMVRLFFIEQLYRGFTILRNEPYHHQ"
     gene            complement(2792..3421)
                     /locus_tag="PK218_04535"
     CDS             complement(2792..3421)
                     /locus_tag="PK218_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97806.1"
                     /translation="MKNVIVFFSFLLICSCQKKEVLLPQISETIVADVKDHSPIYMFF
                     ETKEMDTLIAVNRKNTIISTNWLFNIDKRLPLKLVVPEIKKLQEKKENSEHKNKAAEN
                     YFTYMDSEKKTLAFMPFTETVYKLVKPTFGVIVFIKSISEIRVNGVKVNKEDLQSYLI
                     SIESDKPNKFQFCFSKELLFEDYLKATIFLKSLKFQLPILQTSNQEFIY"
     gene            complement(3418..4236)
                     /gene="folP"
                     /locus_tag="PK218_04540"
     CDS             complement(3418..4236)
                     /gene="folP"
                     /locus_tag="PK218_04540"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPW97807.1"
                     /translation="MTISCLGKLIDLSSPKVMGILNLTPNSFYDGGKFKNEKSILNQV
                     DKMLSDGATFIDIGAYSSKPNAEFVSEKEEENRLFPVIELILKEFPETLISIDTFRSA
                     IAKKAIENGACIVNDISAGSLDDKMFETIANLQVPYIMMHMKGNPQTMQGLAHYENIV
                     KEMLFYFSEKVASARSFGINDLIIDPGFGFAKKMEHNFEVLNNLELFQMLELPILVGF
                     SRKSMIYKTLETSVEFALNGTTVLNTIALQKGAKILRVHDVREAIECVKLVSQL"
     gene            4383..6239
                     /locus_tag="PK218_04545"
     CDS             4383..6239
                     /locus_tag="PK218_04545"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPW97808.1"
                     /translation="MIKKIYSSIALFSLVFVSAQTTIPTPTNIQVTYDKKTRSQTGLP
                     GENYWQNKADYTITVSFDPKTLLLKGSENIYYTNNSNDTLKVIEFKLYPNFFKKGSPR
                     LMKVATDNVSDGISIENFMANNEKIDVKKINIDATNMTVRIPALYPKQSINFSVDFSF
                     TLNEGSNFRTGKIDNGAYFLAYFFPRIAVYDDIDGWNKNQYLGTQEFYNDFCDFKMAL
                     TVPNDYMVWATGDLLNCNEVYQKTYCERINYAETNDEIKYIISSEDIKNGTITRKEKA
                     NNTWQFEAKNVTDIAIALSNHYVWQSSSIEVDRKTKRRTRVDAVYNEIHKDFEFVASD
                     ARKTVEAMSFSFPKWPFPYNHETVFDGLDQMEYPMMVNDNPIEDRAESIELTTHEIFH
                     TMFPFYMGTNETKYGWMDEGWATIGEWIITPMIDNRFVDEYGIEPTSRAAGKEIDLPI
                     TTLTTQMNGETMFLNSYPKPAFAYLFIKDMLGDELFFKGLHYYIEKWNGKHPMPNDFF
                     YCMNVGSGTDMNWFWKKWFFGTGILDLGIKSVKGNQITIENKGEKPLPIDLTITFEDG
                     KVEKIHQSIIAWKKGNKTVLISTKSNNKIKRVDLGSTYVPDSNKANNSFEVK"
     gene            6351..6896
                     /locus_tag="PK218_04550"
     CDS             6351..6896
                     /locus_tag="PK218_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HPW97809.1"
                     /translation="MSQTSQEYDNVIAICRQLYTNKLKDYGCAWRILRLPSLTDQIFI
                     KAQRIRSLQQNQVRKVDEDETGEFIGIINYAIMALIQLDLGVVEQPDLKVEKAVALYD
                     EKVALTKSLMENKNHDYGEAWREMRVSSLTDLILQKLLRVKQIEDNSGKTIVSEGIDA
                     NYQDMINYAVFALILMKFGQK"
     gene            6907..8016
                     /locus_tag="PK218_04555"
     CDS             6907..8016
                     /locus_tag="PK218_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tlpB"
                     /protein_id="HPW97810.1"
                     /translation="MTSKNNIAWGIRIVISFLFLLSAVAKLYPSPYFAISTFEVKQLY
                     PMGFSEGFAPYFSRILIGIELALGVLLLQKNFLRSIVIPGTILLLAVFTTHLTIDSFM
                     NGGNSGNCGCFGSLLPMTPIEAIIKNVVAMILLVWLFIILPKTNEKKNNFWVLSTVTF
                     AAILALFMLAPIQPIATEIPEMTVNENLIEIIDSISKPEIEKVPITIDTVRKTEKIVI
                     KDTSKQETKPEIKSEPEMHKSGFAQYFPTIDKGRKTLCLFVPGCDHCREAAKELTDLR
                     KKIKNLPEISIIFMNEEADLIPDFFKFAGAEYPYKIIEVIPFWKALGKGRDTPGIKYL
                     WNGNEYKYYDGINENKFDIIEYQKIIQKPFEQLMK"
     gene            8031..8339
                     /locus_tag="PK218_04560"
     CDS             8031..8339
                     /locus_tag="PK218_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97811.1"
                     /translation="MLKVFKHKNEKNKAIFGLIVCAIVLIIGYLILRFIATQEEPPLG
                     NTFQILLGTTLCSVSGLGIILIIKYMYDSNKRRKRRQNKRKKHKVVFLKKETLEKKKI
                     "
     gene            8347..8844
                     /locus_tag="PK218_04565"
     CDS             8347..8844
                     /locus_tag="PK218_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPW97812.1"
                     /translation="MKKIVTLFFGALLSISCSNAQKKEFSKESLTKILVSTDKTEISF
                     GDILEKQKGKVTVIEIWASWCSDCVKAMPKVKEMQANNPEVDYVFISMDKVYDKWING
                     IEKHDLKGKHYWATDGMKGKFGKSIDVDWIPRYIILDKNGNIKIYRAIETDFDKINAT
                     LKELK"
     gene            8844..9596
                     /gene="tpiA"
                     /locus_tag="PK218_04570"
     CDS             8844..9596
                     /gene="tpiA"
                     /locus_tag="PK218_04570"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPW97813.1"
                     /translation="MRKKIVAGNWKMNKNSEETEDLLNDLIDKLPNDVEAQIIVAPTF
                     VNLASAVDHLEFTNIVVAAQNMHQNENGAFTGEISADMLKSIGVNTVILGHSERRAYF
                     HETDTLLAQKVTTALIHDMDIIFCFGEELKDRQNNQHFNVVENQLRDGLFHIQNNDWE
                     KIILAYEPVWAIGTGETASPEQAQEMHEFIRETVVKRYGSTIADNVSILYGGSVKPEN
                     AKEIFSKPDVDGGLIGGAALKASDFAKIVEGI"
     gene            9747..10214
                     /locus_tag="PK218_04575"
     CDS             9747..10214
                     /locus_tag="PK218_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97814.1"
                     /translation="MKIKFSLLLIVLTCTSIFAQLDNQKRLQSDQRKYYTWNYEEKKY
                     ELKETEYEHSIIDIREIGSKTNGYIVISMLDNGLTRLQHGSIYSYTQNSENEGTWLFK
                     SKNSKAKITYNPKENTLTYLFDADNERYRRLMIFNVVPDELPDSSLRTVVKID"
     gene            10280..11113
                     /gene="prmA"
                     /locus_tag="PK218_04580"
     CDS             10280..11113
                     /gene="prmA"
                     /locus_tag="PK218_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPW97815.1"
                     /translation="MSNSYLGFHFEVAPRELGSEILIAELGEKAFESFIETENGFSAY
                     IQKEFYTEDILNDIYLLSSPEFKISYTIEEIEQVNWNEEWEKNFEPIDVDGKCHVRAP
                     FHPKTTAEFDIIIEPKMSFGTGHHETTHMMIQHLLETDVKNLKTLDMGCGTAILAILA
                     EMKGAKPIDAIDIDNWCYLNSIENAERNNCKQITVYEGDASLLKGKKYDIIIANINRN
                     ILLNDMQTYVNCLNKNGTLLLSGFYSQDIPFINESCVEKGLTYVKKFERNNWVSLKYV
                     N"
     gene            11146..11421
                     /locus_tag="PK218_04585"
     CDS             11146..11421
                     /locus_tag="PK218_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HPW97816.1"
                     /translation="MSTIEKIKEDVLIEELTGVNNEIVLYNDDVNTFDHVIETLIRVC
                     NHCSEQAEQCAILVHYKGKCTVKTGQFDELKPQCTQLLQAGLSAEII"
     gene            complement(11414..11839)
                     /locus_tag="PK218_04590"
     CDS             complement(11414..11839)
                     /locus_tag="PK218_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97817.1"
                     /translation="MEINLKNGIDSLLFGMKQKDVEQLYGLPNKKFKDEDDNQIYLYN
                     DKKLRLTFYADEDFKLGYIISSNPENTLFNKKIIGENVIKIKNQLPFKSWEIEDFDST
                     ENHFNESNWLILQSEYEEIIRIEIGAIIKDNDEFDWKFK"
     gene            complement(11860..12261)
                     /locus_tag="PK218_04595"
     CDS             complement(11860..12261)
                     /locus_tag="PK218_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HPW97818.1"
                     /translation="MENYEEKIALLTEMIAFSVVDGKLHEREYLFLSMIANELEISNT
                     DFKSLFHQENNAVVIKSEFERIQQFYRLALLMHCDGELHEKEQIKIHEIGINMGLNPH
                     AIKRVLKAMEKSPTNMISPKFLMEVFQEQLN"
     gene            12321..13121
                     /locus_tag="PK218_04600"
     CDS             12321..13121
                     /locus_tag="PK218_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPW97819.1"
                     /translation="MKKIFILSLFLQFIFCVAQENSASKNVSTFTIESKKLKSIKKIW
                     VYLPENYATSTKKYPVIYMHDGQNLFDAKTSYVGEWNVDETLDSIHAQVIVIGIEHGG
                     DKRIDELTPCKHPKYNGGNADSYLDFIVNTLKPEIDQKYRTKTNAKNTAIFGSSLGGL
                     VSFYAALKYPNVFGKVGCFSPSFWFCRDELNQLMTKNKEFDTKIYFLCGDNEGDDDVI
                     RDMETVEKWVNTKRCECKKKNKKVIVKGGQHNEKLWREHFKKAYLWLF"
     gene            13157..13573
                     /locus_tag="PK218_04605"
     CDS             13157..13573
                     /locus_tag="PK218_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HPW97820.1"
                     /translation="MTNNDIFKKLRVALQLRDDQIEEILMLVDFRMSKGEIGNFFRNA
                     DHPKYVECGDQVLRNFLNGLVIHLRGTKENPKNAMDVIRKNQEDVKSNTSISSKPVAP
                     IKKPVDFKKKEVSKKKKPTPKTQIVEKVIYKNGKKK"
     gene            13582..14040
                     /locus_tag="PK218_04610"
     CDS             13582..14040
                     /locus_tag="PK218_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HPW97821.1"
                     /translation="MEKLKHYINRIEEKLPLYETSNLAVSKSAIGWHIDHSFKVINAV
                     VTALKNSKPEEYQWKFNKIRLLIYTIGVFPRGKVKAPKSVQSYETIFLNDLILQFETA
                     KSGLLTLKHLDKNANFNHPIFGQLNLKQTIYFLQLHTKHHLKIMDDILKK"
     gene            complement(14213..15097)
                     /locus_tag="PK218_04615"
     CDS             complement(14213..15097)
                     /locus_tag="PK218_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HPW97822.1"
                     /translation="MELLDGKKISNDIKNEIAEEVNKIKANGEKVPHLAAVIVGNDGA
                     SLTYVGSKVKACERVGFESTLIKMPSTTSETELLKKIKELNENDAIDGFIVQLPLPIQ
                     IDTQRVLMAIDPSKDVDGFHPENFGKMALDMSTFIPATPFGILELLERYNVETKGKHT
                     VVIGRSHIVGRPMSILMGRKGFPGNSTVTLTHSYTKNIAQITTQADIIITALGVPNYL
                     KAKMIKDDAVVIDVGITRVVDETDPRGYKITGDVDFEFVSKKASYITPVPGGVGPMTI
                     AMLLKNTLLAREQKRAKD"
     gene            complement(15159..16496)
                     /gene="ffh"
                     /locus_tag="PK218_04620"
     CDS             complement(15159..16496)
                     /gene="ffh"
                     /locus_tag="PK218_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPW97823.1"
                     /translation="MFNNLSEKLDKALHVLKGHGKITEVNVAETLKEVRRALLDADVN
                     FKIAKDFTTRVKDKAIGENVLTTLQPGQLMVKIVKDELTELMGGDATGINLSGNPSVI
                     LMSGLQGSGKTTFSGKLANYLKTKKGKKPLLVACDIYRPAAINQLHVVGDQIGVEVYS
                     EPENKNPVQIAQNAINHAKSNGFNVVIVDTAGRLAIDEQMMNEIANVHAAIKPQETLF
                     VVDSMTGQDAVNTAKAFNDKLNFDGVILTKLDGDTRGGAAISIKSVVDKPIKFIGTGE
                     KMEAIDVFYPARMADRILGMGDVVSLVERAQEQYDEEEARKIQKKIAKNEFGFDDFLS
                     QIQQVKKMGNMKDLVGMIPGAGKALKDVEIEDDAFKHVEAIIYSMTPQERTKPSIIDM
                     KRKTRIAKGSGRKIEEVNQLMKQFDQMSKMMKMMQGSGGKNLMRMMGGMKGMK"
     gene            complement(16629..17096)
                     /locus_tag="PK218_04625"
     CDS             complement(16629..17096)
                     /locus_tag="PK218_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97824.1"
                     /translation="MKKLFLIPMLFCLFMLNVASMCSSDDDSSSSSQDPTPVINAASQ
                     GTWRVTSYIDSGNDETNHFTGYNFTFGSNNVLTTTNGTNSYTGTWSVTSSDDSNDDNP
                     SSDLDFNILFSSPADFADLTDDWDIVTYSSNTISLIDVSGGNGGTDILVFTKN"
     gene            complement(17229..18230)
                     /locus_tag="PK218_04630"
     CDS             complement(17229..18230)
                     /locus_tag="PK218_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97825.1"
                     /translation="MLISIIGCQKEEETIIQDDTQTFTSSSALSGLLLRTSQFPTAFD
                     NVLDSSSCFSVQLPVTVIVNNQQIVVSNQLDYQLVQNAINQFSNDDDIVNFVYPITIV
                     YQNFQTQILQNSNDLDDVLDECDEDDGFDEIDCININYPIVLNIYDSNNQVANTITIT
                     SNSMLFNFLSNTNSSTLLAVVFPISVTNSNGQNVVINNNAELENFIEDSISDCDDDSN
                     GGSNNTTLTQIFTSGTWYISYFFDDVDETNYFNGYNFTFNSNGSSLAIKNSNTISGTW
                     NIYNDSGAEKIELSFDGLLLDEIEDDWRIIEYTPTLIKLKHVSGGNGGIDYLTFTKN"
     gene            18365..18886
                     /locus_tag="PK218_04635"
     CDS             18365..18886
                     /locus_tag="PK218_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW97826.1"
                     /translation="MKNNSFSNTCEENIFSDFFIKNIKSLRNFLFYKFGNEKQAEDLA
                     QDAFIKLWQNCKDVPIEKAKSYVYTIANNASLNVKAHEKVALKYSQNNAQKSSTNESP
                     EFLLEEEEFKNKLLKAIENLNEIHRTTFLMNRIDGKKYTEIAEELNISVKAVEKRIHT
                     ALVELRKEFSNFK"
     gene            18936..19844
                     /locus_tag="PK218_04640"
     CDS             18936..19844
                     /locus_tag="PK218_04640"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPW97827.1"
                     /translation="MEENYPLADWLNNETSDETLKNDFSEDDINILSKIKKYSVQLET
                     PAFDEQKVLTNILSSPKQKVVHLNYNWIFKIAALFIIILGLYFSFTFLSNTNFTAENG
                     SETALILPDESEVILNSGSNLSYKKWNWENNRLIELEGEAFFKVAKGKKFEVNTTSGK
                     VTVLGTQFNVKQRNNRFEVTCYEGKVKVTHRDKIVILTKRMKTIFENDKNIDVPNTSI
                     NQPEWINDEIVFYKENLNNIISEIERKYNVTIELNSNNNQQVFSGNIPSNDLDVALEI
                     LTKTYHLKIAKKTTNKITLTEINVQN"
     gene            19831..>21414
                     /locus_tag="PK218_04645"
     CDS             19831..>21414
                     /locus_tag="PK218_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPW97828.1"
                     /translation="MFRIRTFYSVFCILISLFYSYAQVEKQALPLKEVLAQISKQHDV
                     KFNYIEDEIILFTLFPPKKELNLEQKLNYLSNETKLTFHKISSKYYTISNDKKLDKPL
                     CGFLINDETKIGIENALIIIENTNTSTFSDANGYFELPKISSNTIKIEHQGFENFEIN
                     PEDLYVINCPNFYLKPIIQTLEEVKTGLYLTTGIYKRNDGSIIIKPQNFGILPGLTEP
                     DVLQTMQQIPGIISIDETISNISVRGGTHDQNLFLWNDIRMYQTGHFFGLISAFNPTL
                     SHTISITKNGSSAFFGESVSSLVTLSSRSENNEKPTTTIGSNMISAEFYKKLKLSKTT
                     SITISGRRSLTDFFNSPTFKNYSKRIFQNTEITDLSTNQIVDYNNNVDFYFYDASIQL
                     QQKIGKKNELNLDLITIKNHLDFDEYSTTDSSRSELKQTNFGGSLSWKTNWNEKNSTQ
                     IISSFSNYNLISENQTLESNQTLNQENKVLDLALKLQHNYAVSNKFKLGFGYQINEIG
                     VTNFDEINLPIFSRTITNVL"
BASE COUNT         7923 a         3223 c         3180 g         7088 t
ORIGIN      
        1 aactttaagt aaaaaaacac aaatcactaa ctttcaacaa acaacatcaa ttgttaatta
       61 aattgtttac aacaataatt ttggctacac caaaaaagga tgtgtttaac tacttttatt
      121 aagtataatt taaacctact attatgcatt tcgatcacga agaagaagac cgtaatttat
      181 ccctttcaaa attcgaatcg atgttgaaaa ccaacaaagt attctttttt gattcagaag
      241 agtttgagga aatcatactg cattaccttg atatgggaaa agcaacttta gccaaaaaag
      301 ccttaaagtt aggtttagaa cagcacccaa aatctaccgg gttgaaatta gttcaagtag
      361 aaatgttgat ttacgatgaa aaactcgacc aagctgaaaa aatgcttaac gagttgtatg
      421 cgcttgaacc tacaaatgaa gaaatataca ttttaaaagc caatatctac tcaaaaaaag
      481 acaagcacga aacggcagta gaactgttag aaaccgcatt acaatattcc gaagatcatg
      541 ccgatattta taacttagtt ggcatggaat atctttttat ggacaatctt gaaatggcaa
      601 aaatcaattt catcaaatgt cttgaagaag attttgaaga tcaatcaggc ttatacaatg
      661 tagtctattg ttttgagttt ttggatcaaa accaagaagc tatcgattat ttaaaaacct
      721 atattgaaaa aaatccatat agtgaaattg cttggcacca aactggtcgc ttatactatg
      781 gtttaaaaaa ctatgagtta gcagcaacat cttttgaatt ggctacttat attgacgacg
      841 aatttatagg tgctttctta gaacgtggaa aagctttaga acgactaaaa agatatgatg
      901 aagccattgt gagctacaat caaacgatag tattagacga tccaacttcg tatgcattat
      961 tgcgtattgg aaaatgttat gaaaaacttg gcaataaagt ggaagctcta aaatattata
     1021 acaaaacagt acatgaagat ccacttttag acaaaggttg gattgctata accgattttt
     1081 acgttagtca aaaaaatcat caaaaagcat tgtattatgt taacaaagca ttggcaatag
     1141 atgaccaaaa tcgtttatat tggaagcgtt ttgcaacgat aaacaaagca atgaacaata
     1201 ttgaagaagc tgaatatggc tatagaaaag ctgtagaatt gggcgatttt caattagaca
     1261 cttggttatt ttgggttgac atcatgaaaa tcatgggtga acacaacaat ggtattttaa
     1321 ccttaattca agcggcagaa tattttccag atcaatttga aattgaatac cgtatggctg
     1381 gtttccactt tttactgaac gaagaagaaa aaggaaattt ccatttaaac aatgctttac
     1441 gattaaattc aaaacatgtt tctattttag aagaattgtt tccattggtt tgggatagag
     1501 tatctgtaca agagtatatt tcaaaacaca aaaagtcaga ataaatgtcg aaaagacagt
     1561 ttggtcttat cggtaaaaac atttcgtatt ctttttcaaa aaaatatttt acggaaaagt
     1621 ttgctttagg caatcttgaa gattacttct atgagaattt tgatttacaa tcaattgatg
     1681 aattccctga aattttaaaa aataattctg acttgaaagg tttgaacgtc acaattcctt
     1741 ttaaagaaac aatcattcct tatttagaca aattgtctaa aaatgctcaa aaaattggtg
     1801 ctgtcaatgt gattcgtttt acaaaaaaag gaagtttaaa aggctacaat tccgattatt
     1861 atggatttat aaaatcttta aaaccattac ttaaagaaca tcacaaaaaa gcattaattt
     1921 taggaactgg cggtgcatca aaaggaattg cttttggatt agagaaatta ggaatacttt
     1981 acacttttgt ttctcgcgaa gaaaaagaag gcatgattaa ttataacaga attaacgcta
     2041 ccacttttga caattacaaa atcattatca attgcacacc attaggcact agtccgaacg
     2101 tgaaagaatt tcccaattta ccttatcaat ttttcaccaa aaagcatatt gcattcgatt
     2161 taatatacaa cccagaagaa actcagtttt taaaaaaagc taaaaagaaa ggtgctacta
     2221 taaaaaatgg ctttgaaatg ttgcaatacc aagctgaaaa atcatggaaa atttggaata
     2281 aataatgaac atcaaattac tagctatagg aaaaacagac aacaaatcac ttcaaacttt
     2341 gattgacgat tataccaaac gtttgtcttt ttatataaaa ttcgatttag aaattattcc
     2401 agatataaaa aatgtcaaaa acctttctga agcacagcaa aaagaaaaag aaggcgaatt
     2461 aatattaacc aaactcacac caacagacca attaattctt ttggatgaaa acggaactac
     2521 tttttcgagt gtaggttttt cggaatattt acagaaaaaa atgaactcgg gtgtgaagac
     2581 tttagttttt gtaattggtg gaccatatgg tttcagcgat gaagtgtata gaaaagcaca
     2641 aggaaaaatt tcattatccg aaatgacttt ttcgcatcaa atggtacgtc tgttttttat
     2701 tgaacaattg tatcgagggt ttacaatttt gagaaatgaa ccctatcatc accagtagaa
     2761 agtacgattt acgaggttgg aagtacgaag tttaataaat aaattcttga ttagaagttt
     2821 gaagaattgg caactgaaat tttaatgatt ttaaaaatat tgttgctttt aaataatctt
     2881 caaaaagtaa ttctttactg aaacaaaatt gaaatttatt tggtttatca ctttctattg
     2941 aaatcaaata cgattgtaaa tcttctttat tcaccttaac tccattaact ctgatttcag
     3001 aaattgattt aataaataca attactccaa atgttggttt tactaattta tatactgttt
     3061 cagtaaatgg cataaaggct aacgtctttt tctcactatc catataagta aaatagttct
     3121 cagcagcttt gtttttgtgt tcactatttt cttttttttc ttgaagtttt ttaatttctg
     3181 gaaccactag ttttaaaggt aaacgtttgt caatattgaa caaccaattg gtagaaatga
     3241 tagtgttttt tctatttaca gcaatcaaag tatccatttc ttttgtttca aaaaacatat
     3301 aaattggtga atgatcttta acatcagcaa caatcgtttc tgaaatttgt ggcaacaaaa
     3361 cttctttttt ttggcaagaa caaatcaata aaaaactaaa aaatacgatt acatttttca
     3421 taattgacta actaatttta cacattctat agcttctctt acatcgtgaa ctcttagtat
     3481 ttttgcacct ttttgcagtg caatcgtgtt taaaaccgtg gttccattta aagcaaattc
     3541 aacagatgtt tctaatgttt tatatatcat tgattttctt gaaaaaccta ctaatattgg
     3601 cagttccaac atttgaaata attctaaatt attcaatact tcaaaattat gctccatttt
     3661 ttttgcaaat ccaaaacctg gatcaatgat taaatcgttg atgccaaaac ttcttgctga
     3721 cgccactttt tcagaaaaat aaaacaacat ttctttgact atattttcat aatgtgccaa
     3781 accttgcatt gtttgcggat ttcctttcat atgcatcatg atgtatggaa cttgaagatt
     3841 tgcaatagtt tcaaacattt tatcatccaa acttccagca gaaatatcat tgactatgca
     3901 agcaccattt tcaatagctt ttttggcaat agcacttcgg aaggtatcta tcgaaattaa
     3961 agtttcagga aattctttta aaatcaattc tattactgga aacaatcgat tttcttcttc
     4021 tttttcagaa acaaattcgg cattcggctt actagaataa gctccaatat cgataaaggt
     4081 agcaccatca gacaacatct tatctacttg atttagaata cttttctcat ttttaaattt
     4141 tccgccatcg taaaaagaat ttggagttaa attaagtatt cccataactt tgggtgaaga
     4201 caagtctatt aattttccta aacagctgat tgtcatctct attaaactaa ataaattgtt
     4261 aaatcctggt aaagataaaa tattaaacct aaaattagag atgttgcatt gatttcatgt
     4321 tacaggaatt gattttttgt acatttgaaa acaccatttt ataatggata aaataaaact
     4381 atatgattaa aaaaatttat tcttcaatcg cactcttttc actagtattc gtttctgcgc
     4441 aaacgactat tccaactcca acaaatattc aagtcactta cgataaaaaa acccgttcgc
     4501 aaactggttt gcctggtgaa aattattggc aaaacaaggc agattacact ataaccgttt
     4561 catttgaccc aaaaacactt ttgctcaaag gcagtgaaaa catctattac acaaataata
     4621 gtaatgacac attaaaagta attgagttta aattatatcc aaattttttc aaaaaaggtt
     4681 caccaagatt gatgaaagta gcgacagaca atgttagcga tgggatttct attgaaaatt
     4741 tcatggcaaa caatgaaaaa attgatgtca aaaaaataaa tattgatgct accaatatga
     4801 ctgttagaat tcctgcgctt tatcctaaac aaagcatcaa ttttagcgtc gatttttcat
     4861 ttaccttaaa tgaaggttcc aatttcagaa caggaaaaat tgataacggt gcttattttt
     4921 tggcatattt tttcccacga attgctgttt atgatgacat tgatggatgg aataagaacc
     4981 aatacctagg cacacaagag ttttacaatg atttttgtga tttcaaaatg gctttaactg
     5041 ttcctaatga ttatatggtt tgggctactg gtgatttatt gaattgtaat gaagtttatc
     5101 aaaaaactta ttgtgaaaga ataaattatg cagaaactaa cgatgaaatc aaatacataa
     5161 tttcaagtga agacataaaa aatggaacta ttactagaaa agagaaagca aacaacactt
     5221 ggcaatttga agccaaaaat gtaaccgaca ttgccatagc actaagcaat cactacgttt
     5281 ggcaatcctc aagcattgaa gtggatagaa aaacaaaacg aagaacccga gttgatgctg
     5341 tttataatga aattcacaaa gattttgaat tcgttgcttc agatgccaga aaaacagttg
     5401 aagcaatgag ttttagtttt cccaaatggc cttttccgta caatcatgaa actgtttttg
     5461 atggtttaga ccaaatggaa tatccaatga tggttaacga taaccccata gaagatagag
     5521 ccgaaagtat tgaattaaca acacacgaaa tttttcatac tatgtttcct ttttatatgg
     5581 gaacaaatga aacaaaatat ggctggatgg acgaaggttg ggcaactatt ggcgaatgga
     5641 ttattacgcc aatgatagac aataggtttg ttgacgaata cggtattgaa ccaacttcaa
     5701 gagctgcagg aaaagaaatc gatttaccaa ttaccacctt aaccacacaa atgaatggtg
     5761 aaacgatgtt tttaaattcc tatccaaaac cagcttttgc ttatttattt ataaaagaca
     5821 tgcttggaga cgaattgttt ttcaaaggat tacattacta tattgagaaa tggaatggca
     5881 aacatccaat gcccaatgat ttcttttatt gtatgaatgt tggcagtggt actgatatga
     5941 attggttttg gaaaaaatgg ttttttggaa caggaatttt agatttaggc ataaaaagtg
     6001 tgaaaggaaa tcaaataact attgaaaaca aaggagaaaa acctctacca attgatctta
     6061 ccattacttt cgaagatgga aaagttgaaa aaatacatca atcgattatt gcttggaaaa
     6121 aagggaataa aactgttcta atttctacaa aatcaaataa caaaattaaa agagtggatt
     6181 taggaagcac ttatgttcct gattctaaca aagcaaataa tagttttgaa gtaaaataat
     6241 ttacattttt ttaaaaacct tcaatcttaa tttaggaata agatatagtg ctttgaactt
     6301 caatctatat tctttatttt tggaaaaaat tgatctaaaa gtacataata atgagccaaa
     6361 cttctcaaga atatgacaat gttattgcaa tatgccgaca actttataca aacaaattaa
     6421 aagattatgg ctgtgcttgg cgcatcttaa gattgccttc attaaccgat caaatattca
     6481 tcaaagcgca acgaatcaga agtttacagc aaaatcaagt tcgcaaagtt gatgaagatg
     6541 aaactggaga attcattgga attatcaatt atgctatcat ggctttaatt caattagatc
     6601 ttggcgttgt ggaacaacct gatttgaaag tcgaaaaagc agtggcgtta tatgacgaga
     6661 aagtcgcgtt aactaagtca ttaatggaaa ataaaaatca tgattatggc gaagcttggc
     6721 gcgaaatgcg agtaagttca ttaactgatt taattttaca aaaactttta cgggtaaaac
     6781 aaattgaaga caactctgga aaaaccattg tttcggaagg aatagatgct aattatcaag
     6841 atatgattaa ttatgctgtt tttgcattga ttttaatgaa atttggtcaa aaataaaata
     6901 aaaattatga catcaaaaaa caatatcgct tggggaattc gaattgtaat ttccttttta
     6961 tttttacttt cggcagttgc caaattgtat ccttcaccct attttgcaat ttcaactttt
     7021 gaagtaaaac aattgtatcc aatgggtttt tcggaaggat ttgcacctta tttttcgaga
     7081 attttaatcg gaattgaatt ggcattaggg gtattactac tacaaaaaaa cttcttacga
     7141 agtattgtaa ttcctggaac aatattatta ttagccgttt ttaccacaca tttgaccatt
     7201 gattcgttta tgaatggtgg gaattcagga aattgtggtt gttttggtag tttgttgcca
     7261 atgacaccaa tagaagcaat cattaaaaat gtagtcgcaa tgattttatt ggtctggtta
     7321 ttcatcattt taccaaaaac taacgaaaag aaaaataact tttgggtttt atcgactgta
     7381 acttttgctg caattttagc attatttatg ttagcaccaa ttcagcccat tgcaacagaa
     7441 attcctgaaa tgactgttaa tgaaaatcta attgaaatta ttgattctat aagtaaacct
     7501 gaaattgaaa aagttccaat aactattgat acggtgcgaa aaactgaaaa aattgttata
     7561 aaagatactt caaaacaaga aactaaacct gaaattaaaa gtgaacccga aatgcacaaa
     7621 tcaggttttg ctcaatattt tccaacaatt gacaaaggac gaaaaacgct ttgcttgttt
     7681 gtacctggat gcgaccattg tagagaagca gcaaaagaat tgaccgactt aagaaaaaaa
     7741 attaaaaatt taccagaaat atctattatc tttatgaacg aagaagcgga tttaattcct
     7801 gattttttca aatttgccgg tgccgaatat ccatacaaaa ttattgaagt aattccattt
     7861 tggaaagctc tcggaaaagg tagagatact ccaggaatta aatatttatg gaacggcaat
     7921 gagtacaaat attatgacgg tattaatgaa aataaatttg atattataga atatcaaaaa
     7981 ataattcaaa aaccatttga gcaattaatg aaataaaata ggtttaattt atgctgaaag
     8041 tatttaaaca taaaaatgaa aaaaacaaag cgatatttgg gcttattgtc tgtgcaattg
     8101 tattaataat tgggtatctt attttaagat ttattgcaac tcaagaagaa ccaccattag
     8161 gaaatacgtt tcaaatatta cttggcacaa cattatgttc agtaagtggt cttggaatta
     8221 ttttaattat aaaatacatg tatgattcca acaagagaag aaaaagacgt caaaacaaac
     8281 gtaaaaagca taaagttgtt tttttaaaaa aagaaactct tgaaaaaaag aaaatataaa
     8341 tttagtatga aaaaaattgt aaccctattt ttcggagcat tactatcaat atcgtgttca
     8401 aacgctcaaa aaaaagaatt tagcaaagaa tcattaacaa aaatacttgt ttctactgac
     8461 aaaacagaaa taagttttgg agatattctt gaaaaacaaa aaggcaaagt taccgttata
     8521 gaaatttggg catcttggtg tagtgattgc gtaaaagcca tgcctaaagt aaaagaaatg
     8581 caagccaaca atcctgaggt tgactatgtt tttatttcga tggataaagt atatgacaaa
     8641 tggattaatg gtattgaaaa acatgattta aaaggaaaac attattgggc aactgatgga
     8701 atgaaaggta aatttggaaa atcaattgat gtagattgga ttccaagata tattatactt
     8761 gataaaaatg gaaatatcaa aatctataga gccattgaaa ctgactttga taaaataaat
     8821 gctacactta aagaattaaa ataatgagaa aaaaaattgt tgctggtaat tggaaaatga
     8881 ataaaaattc ggaagaaacc gaagatttat taaatgattt aatagataaa ttacctaatg
     8941 atgtagaagc acaaatcatc gtagctccaa cttttgtaaa tcttgcatct gcagttgacc
     9001 atttagaatt taccaatata gttgttgctg ctcaaaacat gcatcaaaat gaaaatggag
     9061 catttactgg agaaatttcg gcagatatgc taaaaagtat tggtgtaaac actgtcatac
     9121 taggacattc agaacgaaga gcttatttcc acgaaaccga tactctttta gcgcaaaaag
     9181 taactactgc tctaatacac gatatggaca ttattttttg ttttggagaa gaattaaaag
     9241 acagacaaaa taaccaacat ttcaatgttg tagaaaacca acttcgtgat ggattatttc
     9301 atatccaaaa caacgattgg gaaaaaataa ttcttgctta tgaacctgtt tgggcaattg
     9361 gaacaggaga aacagcttcg ccagaacaag cacaagaaat gcatgaattt attagagaaa
     9421 cagttgtaaa aagatatgga agtactattg ccgataatgt ctccatttta tacggaggaa
     9481 gtgttaaacc agaaaatgct aaagaaatct tctcaaaacc agatgttgat ggaggtttaa
     9541 ttggtggtgc cgcattaaaa gcgagtgatt ttgcaaaaat tgtagaagga atttaatata
     9601 ataataacta taaaaaaaca aacctttcat taaattgaaa ggtttttttt atactaaatt
     9661 acaaaaacac actttttatc gattattttt gtattttatc gattaaatat tttacatttg
     9721 tttcacaatg tcaaaatcat actagaatga aaattaaatt ttcccttttg cttattgtac
     9781 taacttgcac cagcattttt gctcaattag acaaccaaaa aagattacaa tctgatcagc
     9841 gaaaatatta tacatggaat tatgaagaaa aaaaatatga attaaaagaa acggaatatg
     9901 aacattccat tattgacatt cgcgaaattg gctcaaaaac aaatggctat attgtaatta
     9961 gtatgcttga taatggttta acccgtctgc aacatggttc tatttatagt tatactcaaa
    10021 attctgaaaa tgaaggaaca tggcttttta aatctaaaaa ttctaaagct aaaataacct
    10081 acaatcctaa ggaaaacaca cttacttatt tgtttgatgc tgataacgaa cgctatagac
    10141 gtttgatgat attcaacgtt gttccagatg aattacccga ttctagtttg cgaaccgttg
    10201 ttaaaattga ctaattatta tttctcaaaa aacaattgat aaacttctgt tgattgttac
    10261 ctttgcaaaa atataatata tgtcaaatag ttatttagga tttcattttg aagttgctcc
    10321 aagagaattg ggttcagaga tattaattgc agagctagga gaaaaagcat ttgaaagttt
    10381 tattgaaact gaaaatggat ttagtgcata cattcaaaaa gaattttata ccgaagatat
    10441 attaaacgac atctacttac tttcttctcc agaatttaaa atttcttata ctattgaaga
    10501 aatagaacaa gtgaattgga atgaagaatg ggaaaaaaac tttgaaccca ttgatgttga
    10561 tggaaaatgc catgtaagag caccatttca ccccaaaact actgctgaat ttgatattat
    10621 aattgaaccc aaaatgagtt ttggaacagg tcatcacgaa actactcaca tgatgattca
    10681 acatcttttg gaaacagatg tcaaaaattt aaaaacatta gacatgggtt gtggcacagc
    10741 aatcttggca atacttgccg aaatgaaagg cgcaaaacca attgatgcta ttgatattga
    10801 caattggtgt tatctaaatt ctatagaaaa tgctgaacgc aataattgca aacagattac
    10861 tgtttatgaa ggtgatgcca gtttgttaaa aggaaaaaaa tacgacatta tcattgccaa
    10921 tatcaataga aatattttgt tgaacgatat gcaaacttat gtaaattgtc taaacaaaaa
    10981 cggaaccctt ttgttaagtg gtttttatag ccaagatatt ccatttataa atgaatcttg
    11041 tgttgaaaaa ggattgactt atgttaaaaa atttgaaaga aacaattggg tttctctaaa
    11101 atatgtaaat tagcaacttc actttttatt caaaaaaaaa tgaaaatgag tacgatagaa
    11161 aaaattaaag aagatgtttt aatcgaagag ttaactggtg ttaacaatga aattgtatta
    11221 tataatgacg acgtaaacac ttttgaccat gtaattgaaa cactaattcg tgtatgcaat
    11281 cattgttcag aacaagcaga acaatgtgca attttagttc attataaagg aaagtgtact
    11341 gtaaaaactg gtcaatttga cgaattaaaa ccgcagtgta ctcagttact ccaagctgga
    11401 ttgagtgctg aaattattta aatttccaat caaactcatc attatctttt ataattgcac
    11461 ctatctcaat tcgaataatt tcttcatatt ctgattgtag aattaaccaa ttactttcgt
    11521 taaaatgatt ttccgtgcta tcaaaatctt caatttccca agatttgaaa ggcaattgat
    11581 ttttaatttt aataacattt tctccaatta tttttttatt aaatagtgtg ttctctggat
    11641 tagaagaaat gatataacct aatttaaaat cttcatcggc ataaaaagtc aagcgtagtt
    11701 ttttatcatt ataaagataa atttgattgt catcttcgtc tttaaatttt ttattgggca
    11761 gaccatataa ttgctcaaca tctttttgtt tcataccaaa aagaagtgaa tcaattccat
    11821 ttttaagatt aatttccatc atttattaac attaagaagt taattcaatt gttcttgaaa
    11881 gacttccatt aaaaattttg gagaaatcat attcgtcggc gatttttcca tcgcttttaa
    11941 aacccgtttt atagcatgag gatttaaacc catattaatc ccaatttcat gaatttttat
    12001 ttgttctttt tcgtgtaatt ctccatcgca atgcattaat aatgccaaac gataaaactg
    12061 ttgaatacgc tcaaactcag atttaataac tacagcattg ttttcttgat gaaacaaaga
    12121 cttaaaatca gtatttgaaa tttcgagttc attggcaatc attgataaaa aaagatattc
    12181 tctttcatgt aattttccat caacaaccga aaaagcaatc atctctgtta atagtgctat
    12241 tttttcttcg taattttcca tccgttcaat tttagtttag ctaaaatatg attttttgtt
    12301 atattgttta aaaaaaactc atgaaaaaga ttttcattct ttcattattt cttcaattta
    12361 ttttttgtgt ggcacaagaa aattctgctt caaaaaatgt atcaactttt actatagagt
    12421 ccaaaaaatt aaaaagtata aagaagattt gggtttattt acccgaaaac tatgctactt
    12481 caacaaaaaa atatccagtt atctacatgc atgatggaca aaatttattt gatgctaaaa
    12541 cttcttatgt tggcgaatgg aatgttgacg aaactcttga ttctatccat gcacaagtaa
    12601 ttgttattgg aattgaacat ggtggagaca aaagaataga tgagctaaca ccttgcaaac
    12661 atccaaaata taatggtggt aatgcagatt cctatttaga ttttatagtc aataccttaa
    12721 aacccgaaat cgaccagaaa tatagaacaa aaaccaatgc taaaaacact gctatatttg
    12781 ggagttcact cggaggttta gtttcttttt atgcagcctt aaaatatcca aatgtatttg
    12841 gaaaagtagg ctgtttctct ccttcatttt ggttctgtcg tgatgaatta aaccaactca
    12901 tgacaaaaaa caaagaattt gataccaaaa tttatttcct ttgtggcgat aatgaaggtg
    12961 atgatgatgt gatccgagat atggaaaccg tagaaaaatg ggtaaatacc aaacgctgtg
    13021 aatgcaaaaa gaaaaacaaa aaagtaatcg ttaaaggtgg acaacacaat gaaaaattat
    13081 ggcgagaaca ttttaaaaag gcgtaccttt ggttgtttta aaaaaattcc gaattacaaa
    13141 ttggaacttg aaaattatga caaataacga tatatttaaa aaactgcgag ttgcactgca
    13201 attgcgtgac gatcaaatag aagaaatttt aatgttggta gattttagaa tgtcaaaagg
    13261 ggaaattggg aatttttttc gtaatgccga tcatccaaaa tatgtagaat gtggtgacca
    13321 agtattgcgt aattttttaa acggtttggt cattcatctt cgaggcacaa aagaaaatcc
    13381 gaaaaacgct atggatgtca ttaggaaaaa tcaggaagac gttaaaagca acaccagcat
    13441 ttcttcaaaa ccagttgcac caattaaaaa acctgttgat ttcaaaaaga aagaggtatc
    13501 caaaaagaaa aaaccaactc caaaaacaca aattgttgaa aaagtgattt ataaaaatgg
    13561 caaaaagaag tagttcattt tatggaaaaa ctcaaacact atataaatcg tattgaggaa
    13621 aaactacctt tatatgaaac ttcaaattta gctgtttcta aaagcgccat tggttggcac
    13681 attgatcata gctttaaggt aataaatgca gttgtcaccg ctttaaaaaa ctcaaaacca
    13741 gaagaatacc aatggaaatt taataaaatc aggctcttaa tttatactat tggggttttc
    13801 ccaagaggaa aagtcaaagc tccaaaaagt gtccagtctt atgaaactat ttttttaaac
    13861 gatttaattc ttcaatttga aacagcaaaa tcaggtttgt taactttaaa gcatttagat
    13921 aaaaacgcta atttcaatca tcctattttt ggacaattaa acttaaaaca aactatttat
    13981 tttttacaac ttcacaccaa acatcatctt aaaatcatgg atgatattct gaaaaaataa
    14041 accctaaaac tgagaataag taccaccaca aatttcacac attagcacaa atttttcggg
    14101 gtaatttata ttagaaaaca ttttgctgaa aaagagtttc aaacaatttt aatctatttt
    14161 ttttaagact gaaatttgag gtaaaattat tctaaaaaat caggattttg ctctagtctt
    14221 ttgctctttt ttgttctcta gctaaaagtg tgtttttcaa caacattgca attgtcattg
    14281 gacctactcc accaggaaca ggcgtaatgt aagatgcttt tttagaaaca aattcaaaat
    14341 cgacatcacc agtaatttta taacctcttg ggtctgtttc atcaacgaca cgtgtaattc
    14401 caacatcaat tactacggca tcatccttaa tcattttggc ttttaaataa tttggaaccc
    14461 ctaaagctgt gataataata tctgcttggg tagtgatttg cgcaatattt ttagtataac
    14521 tatgtgttag agtaaccgtt gaatttccag gaaaaccttt tcttcccatc aaaatactca
    14581 taggacgacc aacaatatga cttcttccaa taactacggt atgttttcct ttggtttcaa
    14641 cattgtaacg ctctaataat tctaggattc caaatggagt tgctgggatg aatgtactca
    14701 tatctaaagc catttttcca aaattttcag gatgaaaacc atcaacatct ttacttgggt
    14761 ctattgccat caaaactctt tgtgtatcaa tttgtattgg caaaggcaat tgcactataa
    14821 acccatcaat agcatcattt tcgtttaact ctttaatttt ttttagcaat tctgtttccg
    14881 aagtagtact gggcattttt attaaagttg actcaaatcc tactctctca catgctttta
    14941 ctttgctccc aacataggtt aaacttgcac catcattccc aacaataact gcagctaaat
    15001 gaggcacttt ttcaccattt gcttttattt tatttacttc ttcagcaatt tcgtttttga
    15061 tgtcgttgga gattttcttt ccgtctaata attccatttg aagttagtgt gtgtgtgtgt
    15121 ttataaaaaa ataagacgcg ataaatcgcg tctctacatt atttcattcc tttcatgccg
    15181 cccatcatgc gcatcaaatt ctttcctcca gaaccttgca tcatcttcat cattttgctc
    15241 atttggtcaa attgtttcat caattgattg acttcttcaa tttttcttcc agaacctttt
    15301 gcgattcggg tttttcgttt catatcaata attgaaggtt ttgttcgttc ttgaggcgtc
    15361 attgaatata taattgcttc aacatgttta aaagcatcgt cttcaatttc aacatctttc
    15421 aatgcttttc cagctccagg tatcattccg actaggtctt tcatgttacc cattttcttt
    15481 acttgctgaa tttgagataa gaaatcatca aaaccaaatt cgtttttggc aattttcttt
    15541 tgaatttttc gagcttcttc ttcgtcatat tgttcttgtg cacgttcaac caaggaaaca
    15601 acgtcaccca ttccaagaat tctatctgcc atacgtgcag gataaaacac atcaatagct
    15661 tccatttttt cacctgttcc gatgaattta attggtttat caactaccga tttaatagaa
    15721 atagctgctc caccgcgagt atcaccatct aatttggtta aaataactcc gtcaaaattt
    15781 aatttatcgt taaacgcttt tgctgtattt accgcatctt gacctgtcat cgaatccaca
    15841 acaaacaatg tttcttgtgg tttaattgca gcgtgcacat tagcaatttc gttcatcatt
    15901 tgctcatcaa ttgctaaacg accagccgta tcgacaataa caacattgaa accattggat
    15961 tttgcatgat tgattgcatt ttgagcaatt tgaactggat ttttattttc tggttcagaa
    16021 taaacctcaa caccaatttg gtcaccaact acatgcaact ggttgattgc cgcaggacga
    16081 taaatatcac acgctactaa aagtggtttt tttccttttt tagtttttaa ataattggct
    16141 aattttccgg agaaagtagt tttacccgaa ccttgtaaac ctgacatcaa aatcacagat
    16201 ggatttccag aaagattaat tccagttgcg tctccgccca ttaattcggt taattcgtct
    16261 ttaacgattt taaccattaa ttgtcctggt tgtaacgtag tcaatacgtt ttcaccaatt
    16321 gctttgtctt ttacacgagt agtaaaatct tttgcaattt taaaattcac atcggcatcc
    16381 aataaagcac gacgcacttc ttttaaggtt tcggcaacgt ttacttctgt aattttacca
    16441 tgtcctttta aaacgtgtaa tgctttatct aacttttcgc ttaaattatt aaacataatt
    16501 ttcttttttt aagaagtgca aatataagtt ttaagttgca gagttgcaaa gtagaaaaaa
    16561 gtattttcaa caaaaaaacc agctttttaa agctggtttt actaactatc attaaacgaa
    16621 cacaaaaatt aatttttggt aaaaactaaa atatccgtac ctccatttcc tccactaaca
    16681 tcaattaacg atatggtgtt agaactgtaa gtaactatat cccaatcatc cgttaaatca
    16741 gcaaaatctg caggtgaaga aaatagaata ttaaagtcta aatcactact tggattatcg
    16801 tcattactat catcagaaga agttactgac catgttcctg tatatgaatt tgttccatta
    16861 gttgtagtta atacgttatt acttccaaaa gtaaaattat aacctgtaaa atgatttgtt
    16921 tcgtcattac ctgaatctat gtaacttgtt actcgccaag ttccttgtga agcagcattt
    16981 attactggtg ttggatcttg tgaagatgag cttgaatcat catctgaaga acacattgat
    17041 gctacattta gcataaaaag acaaaaaagc attgggatta aaaataattt tttcatgatt
    17101 taaaatttag aatattaatt tgatttaagt tttattatat atatggttaa tcctagtatt
    17161 acagtccaaa ttattaattg gataatagga taatgtgttt tattgttttt gtttgattca
    17221 atcatgattt agttttttgt aaacgtcaaa taatcaattc caccgtttcc accactaaca
    17281 tgttttagtt taattaaagt tggtgtatat tcaattattc tccaatcatc ttcaatttca
    17341 tccagcaata atccatcaaa actcaattct attttttcgg ctccgctatc attatatata
    17401 ttccatgtac cgcttatagt atttgaattt ttaattgcca gtgatgatcc gttgctatta
    17461 aatgtgaaat tgtatccatt aaaataattg gtttcatcaa catcatcaaa gaaatatgaa
    17521 atataccaag ttcccgatgt aaaaatttgg gttagtgttg tattattgct tccaccatta
    17581 gaatcatcgt cacaatctga tatagaatct tcaatgaaat tctcgagttc agcattatta
    17641 tttatcacaa cattttgacc atttgagttt gttacagaaa ttggaaaaac aactgccaat
    17701 aaagtgctag aatttgtatt agaaagaaaa ttaaacaaca ttgaattgct tgtaattgtt
    17761 attgtattag caacttgatt gttactgtcg taaatattta agactattgg gtaattaatg
    17821 ttaatacaat ctatttcatc aaaaccatca tcttcatcac attcatcaag aacatcatct
    17881 aaatcatttg aattttgaag aatttgagtt tgaaaatttt gataaacaat agttattgga
    17941 tatacaaaat ttacaatatc atcatcgttt gaaaattggt tgatagcatt ttgaaccaat
    18001 tgataatcta attgatttga aacaacaatt tgctgattat taactatcac agtaaccgga
    18061 agctgaacac taaaacaact cgaactatcc aaaacattat caaaagcagt aggaaattga
    18121 gaagttctta aaagtaaacc cgataaagca gaactgcttg taaaagtttg agtatcgtct
    18181 tgaatgatgg tttcttcttc cttttggcag ccaattatac ttatcagcat aaaagatatt
    18241 agtattttat aatatttttt cattttaaaa ttgattgctt ttatctatta aacaagttat
    18301 ttgtattttt ccctaccaaa aatttaaaaa aatttaactt tacacaacaa agtcaaataa
    18361 attaatgaaa aacaattcat tttcaaatac ttgtgaagaa aatatatttt ctgatttttt
    18421 tataaaaaac ataaagtcgt tacgcaattt tttattttac aaatttggaa atgaaaaaca
    18481 agcagaagat ttggctcaag atgcttttat aaaattatgg cagaattgca aagatgttcc
    18541 aatagaaaaa gcaaaatcat atgtttatac tattgccaat aatgcttcgt taaatgtaaa
    18601 agcgcacgaa aaagttgcct taaaatattc tcaaaacaac gctcaaaaaa gttctactaa
    18661 tgaatcgcct gagtttcttt tagaagagga agaattcaaa aataaattat tgaaagccat
    18721 cgaaaattta aacgaaattc atagaactac ttttttgatg aatagaattg acggtaaaaa
    18781 atataccgaa attgcggaag aactaaatat atcagtaaaa gctgttgaaa agcgaattca
    18841 cacggcttta gttgaactta gaaaagaatt ttcaaatttc aagtagggta aaaatgtaca
    18901 aaactgtttt ataaacgtaa cttatagaca aatctatgga agaaaattat ccactagcag
    18961 attggctcaa taatgaaact tcagatgaaa cattaaaaaa tgatttcagc gaggatgata
    19021 ttaacattct ttctaaaatt aaaaaatatt cggttcaact tgaaactcca gcttttgatg
    19081 aacaaaaagt tttaaccaat attctttcat ctccaaaaca aaaagttgtt catctaaatt
    19141 ataattggat ttttaaaata gctgcgctat ttattattat cttagggttg tattttagtt
    19201 ttaccttttt atcgaatact aatttcacag ctgaaaatgg ttcagaaacc gctttgattt
    19261 tgcctgatga atctgaagtt atattaaatt caggctcaaa tttaagctat aaaaaatgga
    19321 attgggaaaa caatagattg atagaattag aaggcgaagc tttttttaag gttgctaaag
    19381 ggaaaaaatt tgaagttaac acaacatctg gaaaagttac cgttttaggg actcaattta
    19441 atgttaaaca acgtaataat cgttttgaag ttacctgtta tgaaggaaaa gtaaaagtaa
    19501 cacatcgtga caaaattgtt atcctaacta aaagaatgaa aacaattttt gaaaacgata
    19561 aaaacataga tgttccaaat acgtctatca atcaacccga atggataaat gatgaaatag
    19621 tattttataa agaaaattta aataatatca tttcagaaat tgaacgaaaa tataatgtta
    19681 caattgagtt aaacagcaac aacaaccaac aggttttttc gggaaatatt ccatcgaatg
    19741 acctagatgt agctttagaa attttaacta aaacctatca cttaaaaatt gcaaaaaaaa
    19801 caactaataa aattacttta accgaaatca atgttcagaa ttagaacatt ttatagtgta
    19861 ttttgcattt taatttctct tttttactct tatgctcaag tagaaaagca ggctttgcca
    19921 ttgaaagaag ttttagctca aatttcaaaa caacatgatg ttaaattcaa ttacattgaa
    19981 gatgaaatta ttctgtttac tttatttcct ccaaaaaaag aattaaatct tgaacaaaaa
    20041 ttaaattatt tatcaaacga aacgaaactc acttttcata aaatttcaag taaatattac
    20101 accatttcca atgataaaaa attagacaaa ccattgtgcg gttttttaat taatgatgaa
    20161 acaaaaattg gcattgaaaa tgccttaata attatagaaa acacaaacac aagtactttt
    20221 tctgatgcca atggatattt tgaattgcca aaaatttcat ctaatactat taaaattgaa
    20281 catcaaggat ttgagaattt tgaaataaat cctgaagatt tatatgtcat aaattgtcca
    20341 aatttttatt taaaacctat cattcaaaca ttagaagaag taaaaacagg tttatattta
    20401 accacaggta tttacaaaag aaatgatggt tcgataatta taaaaccaca aaactttgga
    20461 attctacccg gattaacaga accagatgtt ttgcaaacca tgcagcaaat tcctggtatc
    20521 ataagtatag acgaaaccat ttcaaacatc agtgttcgag gcggaacgca tgaccagaac
    20581 ttatttctat ggaatgatat tagaatgtat caaactgggc acttttttgg attaatttcc
    20641 gcatttaacc ctacattatc gcatacaatt tcaattacta agaatggaag ttcagcattt
    20701 tttggagaaa gtgtttctag tttagttacc ttatcttcaa gaagtgaaaa taacgaaaaa
    20761 ccaactacaa ccattggttc aaatatgatt agcgctgagt tttataagaa attaaaatta
    20821 tcaaaaacta cttcgattac catttcagga agaagatcat tgactgattt cttcaattct
    20881 ccaactttta aaaattatag caaacgtatt tttcaaaata ctgaaatcac cgatttaagt
    20941 actaatcaaa ttgttgatta taacaacaat gttgactttt atttttatga tgcgtcaatt
    21001 cagttgcaac aaaaaattgg caaaaaaaat gaattaaatt tagatttaat aactataaaa
    21061 aatcatttgg attttgatga atattcgact actgatagtt ctagaagtga attgaaacaa
    21121 acaaattttg gtggttcatt gtcttggaaa actaattgga atgaaaaaaa ttcgacacaa
    21181 attatttcta gtttttcaaa ttacaatctg atttctgaaa atcaaacttt ggaaagcaat
    21241 caaactttaa accaagaaaa taaagtatta gatttagcat taaaacttca gcataactat
    21301 gcagtttcaa acaaattcaa acttggtttt ggttatcaaa taaatgaaat aggagttacc
    21361 aattttgatg aaattaacct tccaatattt tcaagaacaa ttacaaacgt atta
//
LOCUS       DAOOBH010000026        20604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_3420_length_20604_cov_5.186968, whole genome shotgun sequence.
ACCESSION   DAOOBH010000026 DAOOBH010000000
VERSION     DAOOBH010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 20604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20604
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3420_length_20604_cov_5.186968"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2068
                     /locus_tag="PK218_04650"
     CDS             <1..2068
                     /locus_tag="PK218_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPW97829.1"
                     /translation="HAHTDEIGSFKIDKTVLGDTLIVSALGFIKQRVVVNESTLKVFL
                     QNTIYELEQVSIQPKLNAMNAISKIDLQTNPVNSSQEILRKVPGLFIGQHAGGGKAEQ
                     IFLRGFDIDHGTDIAISVDGMPVNMVSHAHGQGYADLHFLIPETVQKIDFGKGTYYAS
                     KGDFATAGYVAFQTKERTEKNAIGVEVGQFNTFRTLGMFNLIENSKTEDAFLATEYIL
                     TDGPFDAPQDFKRINLFGKYTKRFSNNSKLSIQASRFASNWNASGQIPQRLVDAGVIS
                     RFGAVDATEGGNTSRTNLNVSLLKPINENTFIKANAFYSQYNFELYSNFTFFLEDPIN
                     GDQIRQKEDRAIYGFNAEVSKKKDNAIYQFGLGFRSDETNDTELSHTKNRYETLEQLQ
                     LGDISQSNVFGYINADYTFGKFTVNPSVRVDYFTFGYNDKLATSYQNLSADKYKVSPK
                     MNLIYSINNQFQVFAKSGLGFHSNDTRVVVAQEGKDILPTAFSTDLGTIFKPFPKMII
                     NTALWYLQLDQEFVYVGDAGIVEPSGKTRRMGVELGIRYQLTDWLYFDADANYTYGRS
                     IDEPKGQDYIPLAPDFTTTGGLSFTKFKGFSGGFRYRYLKSRPANEDNSIVAKGYFVN
                     DFNINYEYKNITFGIAIENLLNTKWNETQFATESRLQNETNSVEEIHFTPGTPFFIKT
                     KVVYQF"
     gene            2260..2853
                     /locus_tag="PK218_04655"
     CDS             2260..2853
                     /locus_tag="PK218_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPW97830.1"
                     /translation="MKKILGTIGVFLILASCQNKTQEKTIAMNMDATAAVTTKENIYQ
                     FKVKDLYGKEFDFSTLKGKKVLIVNTASECGLTPQYKDLEAIYKKYSDKNFVIIGFPA
                     NNFGAQEPGSNEQIATFCEKNYGVTFPMMSKVSVKGKDMCDVYQFLTQKSKNGLQDSE
                     VEWNFQKYLINEKGELVKVLSPRVLPTDQQIIDWINS"
     gene            2993..3256
                     /locus_tag="PK218_04660"
     CDS             2993..3256
                     /locus_tag="PK218_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97831.1"
                     /translation="MQDIFNPVYRKDYFEGFSEGLNPMLSLRDSYSDAYYEGFQLGRE
                     EYENLNGKITNGIPKLIVTTKVLEDFLLAGMLGMNIDESGYTP"
     gene            complement(3611..4030)
                     /locus_tag="PK218_04665"
     CDS             complement(3611..4030)
                     /locus_tag="PK218_04665"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPW97832.1"
                     /translation="MATNRTFTMIKPDGVENGHIGGILNMITEGGFRIVAMKLTQLTV
                     ADAKAFYAVHSERPFYGELVEFMTRGPIVAAILEKDNAVEDFRTLIGATNPANAAEGT
                     IRKKYATSMGENAVHGSDSDENAAIEGAFHFAGREQF"
     gene            complement(4095..5687)
                     /gene="bshC"
                     /locus_tag="PK218_04670"
     CDS             complement(4095..5687)
                     /gene="bshC"
                     /locus_tag="PK218_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HPW97833.1"
                     /translation="MTESECISYKKSGYFTKLIIDYLEEKPELKQLYNRFPTVENFKK
                     QIEEKKDNFPLENRAILVDELLKQYQKCTVSEITLNNIKHLNDTNTYTITTGHQLNLF
                     TGPLYFLYKIISVINLCKQLKTSYPDHNFVPIYWMATEDHDFEEINHFQLHEKKIRWN
                     KESFGPVGRLSTTGLEEVFDVFEKELGVGKNADFLKQLFQKGYLEHTNLADATRYIAN
                     ELFKNEGLVILDGDATTLKKLFIPYVKKELLEETSFTKVNETAAILNDYNIQVNPREI
                     NLFYIEETLRERIVNDKGIYKVLNTTIAFSKEELLTELDNHPEKFSPNVILRPLYQEV
                     ILPNLCYIGGGGEIAYWLELKSNFKAFDITFPILLIRNSVVIVTEKQLTKTKKLNLTW
                     TDLFSKQLDLINSKTKELSAVTIDFSAQKEFLKKQFEALYKIAEATDASFIGAVKAQE
                     IKQTKGLDNLEKRLLKAEKRIHHDQLERIKLLQNELFPNQSLQERKLNFSEFYLEAGD
                     RLIQSLLKNLKPLETNFTIITL"
     gene            complement(5696..6652)
                     /locus_tag="PK218_04675"
     CDS             complement(5696..6652)
                     /locus_tag="PK218_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97834.1"
                     /translation="MKRILSIIALAFIINACDDGDLQVDNIDFEDIIAKKCDVKDVIY
                     KIKDSEILFVEIPATIFVNDETLVDAPISIPITGDVKVTYRQYNDIVTLDNICPTVPS
                     ATPNVIEEWRATSGTIEITTTAIKTTDAVTNATKITGYKNYIVFKNIAFQKPSGIQTY
                     DTYVFGNYNTNIAALAFGFDEDNAVKSTCSDVVYNFNGSEVLTFAPADFATLFANEVT
                     TTPRTALIDATNTLSYRLYNNVVDNDHFCTNPTPSTPTLSQQWNAVEGVTNVSGMIEV
                     STTTFGTSFQHTIHLKNVSLKKGNSQFSLGDDYILGKIITNP"
     gene            6746..7867
                     /locus_tag="PK218_04680"
     CDS             6746..7867
                     /locus_tag="PK218_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPW97835.1"
                     /translation="MKKIYLDNAATTPIRQEVIDEMVIVMQSEYGNPSSTHSIGRGSK
                     AIIESSRKIIAKHLNCSAQEIIFTASATEATNWILRCCIKDLGIKRIITSKIEHHATL
                     YTIHALLKEFDIKVDYINILSNGNLDYAHFESLFDNDEKTLVSLMHVNNEIGVINDIQ
                     RIGEWCHQHQAVFHCDTVQSIGKTRFDLQKLPVDFLVSSAHKFHGPKGVGFAFLTKKI
                     GLKPMLFGGEQEKGLRAGTEAVHQIAGLSKALDMSFQNIDKEANYIKEIKEYCWQQLK
                     ENFPEIQQNGENTLYNILNVLLPFSAEKTAMILFNLDMKGIAVSRGSACQSGSIKPSH
                     VLAEMLNENDIKKPSLRISFSHYNTKKEVDVFIEELKRI"
     gene            complement(7964..8800)
                     /locus_tag="PK218_04685"
     CDS             complement(7964..8800)
                     /locus_tag="PK218_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW97836.1"
                     /translation="MKKSIIALSLVALTLTSCGSKKKIAALEAQNKECQDLLNSTTMK
                     LNLCLSEKDALSKQNDYLKQNTSDLINSQKEMTMLTSKGAQNLEKSLESLKEKDLKIS
                     RLQDALTRKDSVTLALVTSLKSSVGISDPDIEINVEKGVVFISIADKLLFKSGSYIVS
                     DKAKGVLAKVAKVINSRPTFECMVEGHTDNVPFTGNAVLLDNWDLSVKRSTSIVRVLV
                     NDLGVKPSQLIAAGRGEYIPLVDNNTVENRAKNRRTRIVVLPKIDEFYDMIEKEMKNQ
                     TK"
     gene            complement(8866..9870)
                     /locus_tag="PK218_04690"
     CDS             complement(8866..9870)
                     /locus_tag="PK218_04690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW97837.1"
                     /translation="MKKIAVVILNWNGEELLEKFLSSVILHSDEASIYVADNASTDNS
                     IQFIKDNYPTISIIHNEANYGFAKGYNQALQLVEEEIYCLLNSDVEVTENWLIPIQHL
                     FEKDNTIGIIQPKILDFKDKTKFEYAGAAGGFIDKYGFPFCRGRIFDTIEADLGQYDD
                     EKEIFWASGACFFIRKEIYRTLNGFDDDFFAHQEEIDLCWRAFNLGFKTKYTSNSVVY
                     HVGGATLQEGNPKKTFLNFRNSLFMLTKNLPENKLIAILFYRLSMDGIAGIQFLVKGQ
                     WKHFLAILKAHFYFYKMINKNLRKRKPNNSQDYYYLKSIVFCYFLKNTKLFTKINNSL
                     "
     gene            complement(9886..10332)
                     /locus_tag="PK218_04695"
     CDS             complement(9886..10332)
                     /locus_tag="PK218_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPW97838.1"
                     /translation="MQKLNFPVYSFRFKNSENKVSIFDPIRKKFILLTPEEWVRQHTI
                     EYLMKEKNYPLSYINVEKLIKINDRNKRYDIVIFKPNGAIFLLIECKAPEISISQATF
                     DQIARYNLVLKAEYLMVTNGLNHYFCQMDFENERYNFLKELPDFIL"
     gene            10406..11407
                     /gene="holA"
                     /locus_tag="PK218_04700"
     CDS             10406..11407
                     /gene="holA"
                     /locus_tag="PK218_04700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPW97839.1"
                     /translation="MDEIVKIINDIKVGNIKPIYFLMGEESYYIDKLTEYLEDNLLTE
                     DEKGFNQMVLYGRDTTIEDIVSNAKRYPMMAERQVIIVKEAQDLSRTIDKIEAYVNNP
                     QPTTVLVFAYKYGKVDKRKKWIKSIAKNHILFESEKLKDFKVADWIKRVLQGRGFSIE
                     PKAAIMLQEFLGNDLSRIANELSKLEIILPKGSTITPDHIEQNIGISKDFNVYELRKA
                     IGEKNHHKAFQILNYFHQNPKSIPKVLLIGQLFGYFSNLLKYHGLKDKTQGNVASALG
                     IHPFFVDEYRIAAANFPMKKVSTVINKLKVVDGMSKGVGASNLEDIELYKEFLISVM"
     gene            11465..12010
                     /gene="pyrR"
                     /locus_tag="PK218_04705"
     CDS             11465..12010
                     /gene="pyrR"
                     /locus_tag="PK218_04705"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPW97840.1"
                     /translation="MSQKILLTSKEVNIILHRLACQLIENHNDFSNTVLIGIQPRGVF
                     LAERIKTILENSYKVKEVTLGFLDITFFRDDFRRGEKVLEANQTKIDFLVENKKVVFI
                     DDVLFTGRSIRSALTAIQSFGRPSTIELLVLIDRRFSRELPIQPDYRGRQVDVINNEK
                     VVVNWSEKDGEDAIYLKEKDN"
     gene            12022..12945
                     /locus_tag="PK218_04710"
     CDS             12022..12945
                     /locus_tag="PK218_04710"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPW97841.1"
                     /translation="MELSVNHLLGIKYLNANDIQLIFETADHFKEVINRPIKKVPTLR
                     DITIANIFFENSTRTKLSFELAQKRLSADVISFSAAQSSVKKGETLIDTVNNILSMKV
                     DMVVMRHANPGAAYFLSQNVKASIINAGDGAHEHPTQALLDSFSIREKLGEVAGKKVV
                     IVGDVLHSRVALSNIYALQMQGAEVKVCGPKTLIPKYIESLGVTVETNLRKALEWCDV
                     ANMLRVQNERMEVNYFPSTREYAQQYGVDKALLDSLNKEIVIMHPGPINRGVEITSDV
                     ADSKQSVILNQVENGVAIRMAIIYLLASKIQ"
     gene            complement(12991..15090)
                     /locus_tag="PK218_04715"
     CDS             complement(12991..15090)
                     /locus_tag="PK218_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HPW97842.1"
                     /translation="MLEEQNDNLPKADGNLIDEPTQSVQEENNDAEIITIVEATSEEV
                     VADEIETQEVLLEETTLDTASEEIITESQPNEVNENVIELTDEQLEDDTFVVETKIKS
                     AVETIEDSNAEESEDETIKERHAIPMLDYDTMSLQQLVEELEKLVAVEKVMSVKDHVE
                     ELKKAFLSEYHHFIEEKKEEFLAENPETTEDFHYHLPLKSTFDHLYSQYRDKKNNHFQ
                     SLQNNLKANLNTRLAIVEELKELINSTETMSTMLKQFNDIRDRWKVAGPIPKDKYNHV
                     WNNYHFHLENFYDILHLDREIRDLDFKHNLEQKQKIIARVEELLQEEDIHSAFRELQD
                     LHRIWKEEIGPVSREVREDIWNQFSDLTKQMHDKRESFYASFREVEEANLAIKKEIIS
                     QLEVLAAEKVTTHNAWQEQIHKVEALRNQFFAAGKVPSDVNEATWFAFKTAVRNFNKL
                     KNSFYKDLKQDQNENLAKKQALVDKARALKDNTDFEATTPLMKQIQEEWKSVGHVPRK
                     LSDKLWKEFRAACNAYFENLKSQRKELDSEEIEAFEKKKEYLETIKSFELVGEHKTDL
                     DAIKAHIETWKSFGKVPFSRRYIEGKFNKILDVLFEKLSSSKKDNDIARYSNKLDHLA
                     SLDSRKIDGEKIFIMRKIDEVQNDIFQLENNIQFFANADANNPLVKEVHKNIEKQKEE
                     LATWKEKLKQLNNFMAE"
     gene            complement(15254..16339)
                     /locus_tag="PK218_04720"
     CDS             complement(15254..16339)
                     /locus_tag="PK218_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPW97843.1"
                     /translation="MKKNFTTLLLLLVSICFAQIPNGYYNSATGTGYILKTQLHNIIK
                     NHNDRGYSGLYVTYTTSDKDFYYENDGTMLDMYTENPTGAECEFTYGINQDDGTLGNA
                     ECERYNREHLIPQSVFGSGVTPMYSDAHFVVPSDKYVNAQRGDLPFSRVNAVTNTYSN
                     GSKKGNNLNSGYSNGYNGTVFEPINEFKGDIARMLLYFATRYEDQVANWNFAMFNNTS
                     NQVFTNQAINVLLTWSNDDPVSQREIDRNNAIYARQNNRNPYIDHPEYAMQIWGSLLN
                     TDQYLLEPTVTISPNPSNDGRFTITTGSFAIDAISVLNVNGQVIQYFKNPAVFDNQYE
                     VSNLSKGFYFITIDSNNQRITKKVIVN"
     gene            complement(16342..17556)
                     /locus_tag="PK218_04725"
     CDS             complement(16342..17556)
                     /locus_tag="PK218_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPW97844.1"
                     /translation="MRLHRNLVYTTIDSLNAIFNEGEYADKVVARALKKDKRWGSSDR
                     KFVAETIYEIVRWKRLYAEIAEVKEPFTREDLWRLFAVWAVLRGYPIPDWRQLEGTPQ
                     RKIKGRFDELSKIRKYRESIPDWMDELGVKELGEEQWTKELAAQNQQAKVILRVNTLK
                     TNREQLRAILMDLNIDTEYLKNQPDTLVLKERANVFLTDAFKQGFFEVQDASSQLVAP
                     FLGVEPGMRVVDTCAGAGGKTLHIASLMENKGQLIAMDLYESKLKQLKIRAKRNGAFN
                     IEYRIIDSTKVIKKLYEKADRVLIDAPCSGLGVLKRNPDSKWKLQPEFIDNIKKVQAE
                     VLESYSRIVKPGGQLVYATCSILPSENDEQIKRFLTTENGKEFTFVKDVKILASESGF
                     DGFYMALLQKNN"
     gene            complement(17657..19621)
                     /locus_tag="PK218_04730"
     CDS             complement(17657..19621)
                     /locus_tag="PK218_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HPW97845.1"
                     /translation="MGSFKHLNLHSKLSTAGLLITLGIIYGDIGTSPLYVMKAILGDY
                     PISRDIVLGGISCIFWTLTLQTTLKYVIITLSADNHGEGGIFALFALVKKMKIKWLIV
                     PAIIGGSALLADGIITPPISVSSAVEGLRLFYPEINTIPIVITILIILFTIQQFGTKI
                     VGKFFAPMMLVWFTMLGVLGILQVVQDVSVLNAVNPYYAYELLTLHPEGFFVLGAVFL
                     CTTGAEALYSDMGHCGRKNIRISWIFVKTMLLLNYFGQGAFLINHEGKTLTELGGNAG
                     NPFYLIMANWFQPAGIIIATMAAVIASQALISGSFTLINEAMRLNFWPKVKIKYPTEV
                     KGQLYIPSINWLLLLGCICIVLYFKESSNMEHAYGLAIVLCMIMTTFLLNFYLIMKRV
                     KLYFIVPLISLYLVIEFSFLYANITKFLHGGFVTLIIAFALIGIMATWYLAKQISKNY
                     TNFVKIEDYKKVLAELSVDLSIPKYATHLVYMTNAQRSDEIEEKVMYSILQKRPKRAD
                     IYWFVHVNILNEPYRKEYKVTEIFSEDLYRVDFYLGFREATKINLMFKEVIKEMVARG
                     EMDISSRYESLNKNNIIGDFKFVLSEKFLSNDSHLTFFEKIVMNTYFILKKLSLSEQK
                     GFGLDSSSVKVEKFPMVLHAPENIEMIRID"
     gene            complement(19834..>20604)
                     /locus_tag="PK218_04735"
     CDS             complement(19834..>20604)
                     /locus_tag="PK218_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HPW97846.1"
                     /translation="LDGKVLKEFLDEKVVQYNNRSFIDTDPIQIPHQFSVKEDIEIAG
                     FLVATLAWGQRKTIINKGKKLMELMGNSPYDFILNHSESDIEKLSDFTHRTFNGIDCQ
                     FYIKSLHNIYTNHSGLETIFTLNQSNTQMHESIVNFRTIFFEIEHWIRTRKHLADPST
                     GAAAKRMHMFLRWMVRHDKNGVDFGLWKNIPMSKLSCPLDVHTGNVARSLGLIDRRQD
                     DIKTVNLLDKKLREFDSDDPVKYDFALFGLGVHEEFRL"
BASE COUNT         6825 a         3346 c         3354 g         7079 t
ORIGIN      
        1 gcacgcacat acggatgaga ttggcagttt taagatagac aaaacggttt taggtgacac
       61 gttaatcgtc tctgctttgg gctttatcaa gcaaagagta gtggtgaatg aaagcacact
      121 taaagtattt ttacagaata cgatatatga attagaacaa gtttcgatac aaccaaagtt
      181 aaacgctatg aacgccatat ctaaaataga tttacaaacc aatccggtga attcttcgca
      241 agaaatactg cgaaaagtgc ccggtttgtt tatcgggcaa catgctggcg gcggaaaagc
      301 agagcaaatc ttcctaagag gatttgatat tgaccacggc accgacattg ccatctctgt
      361 agatggtatg cccgtcaata tggtatcgca tgcgcacgga caaggatatg ccgatttgca
      421 tttcttgatt ccagaaacgg ttcaaaagat agattttgga aaaggaactt attatgccag
      481 caaaggcgat tttgctacag caggatacgt ggcgtttcaa accaaagaac gaaccgaaaa
      541 aaacgctatt ggagtagaag ttgggcagtt caacacgttt agaacacttg gcatgtttaa
      601 tttgattgaa aactcaaaga cggaagacgc tttcttagct accgaataca ttttgaccga
      661 tggaccgttt gatgctccac aagattttaa acgtatcaat ttgtttggaa aatataccaa
      721 acgattcagc aataatagca agttgagtat tcaggcttcc cgatttgcta gtaactggaa
      781 cgcatcagga caaattccgc aacgattggt tgatgcgggt gtaatatcaa gatttggagc
      841 ggttgatgct acagaaggcg gtaacacctc gcgtaccaac cttaatgtaa gcttgttaaa
      901 accaataaac gaaaacacat tcataaaagc caatgcattt tacagtcaat acaattttga
      961 attgtattct aactttacgt ttttcttgga agacccaatt aatggcgacc aaattagaca
     1021 gaaagaagac cgagctattt atggttttaa tgccgaagtg agcaaaaaga aagacaatgc
     1081 aatctatcag tttggactag gctttagaag cgatgaaacc aatgataccg agttgtcaca
     1141 cactaagaat agatatgaaa ccttagagca attgcagttg ggcgacatat cacaatcaaa
     1201 tgtttttgga tacatcaatg ctgactatac gtttggcaag tttaccgtta atccatcggt
     1261 tagagttgat tattttacgt ttggatacaa cgataaattg gcaacaagtt atcagaattt
     1321 atcggccgac aagtataaag tatcgccaaa gatgaattta atatatagca ttaataatca
     1381 gtttcaggtt tttgccaagt caggattagg atttcattcc aatgatacta gagtagtggt
     1441 agcccaagag ggaaaagaca ttttgccaac cgcttttagt actgatttgg gaacaatatt
     1501 caaaccattt ccaaaaatga ttatcaatac tgcactttgg tatttacagt tggatcagga
     1561 gtttgtctat gttggtgatg ccggaattgt agaaccaagc ggaaaaacca gaagaatggg
     1621 tgttgaacta ggaattcgtt atcaattgac cgattggttg tattttgatg ctgacgccaa
     1681 ttatacttac ggaagaagta ttgacgaacc caaagggcaa gattacattc cgttggcacc
     1741 cgattttaca acaactggtg gtttgagttt tactaagttc aaaggctttt cgggtggatt
     1801 cagataccgc tatttgaaat caagaccagc aaatgaagat aattctatcg ttgccaaagg
     1861 ttattttgta aacgatttta atatcaatta tgagtataaa aacataacat ttggtatcgc
     1921 tatagaaaat ttattgaata ccaaatggaa cgaaacccag tttgccaccg aatcgagatt
     1981 gcaaaacgaa accaatagtg tagaagaaat acattttaca cctggaacac ctttttttat
     2041 aaaaaccaaa gtagtgtacc aattttaata acgaaaaaga agatttaggt tttttgtttt
     2101 gattgtagca aaggtttaag gttaggttaa cctttgttat gtttgtttgg cccaatgatt
     2161 cccaagtcat tgggcttttt tttaaaattt aatacaactt tatcatttgt ataacatatc
     2221 aaccctttta taattactaa ttttataaaa aaaaatatca tgaagaaaat attaggaaca
     2281 ataggcgtat ttttaatttt ggcaagttgc caaaacaaaa cacaagaaaa aacaatagca
     2341 atgaatatgg acgcaacagc agcagtgact acaaaagaaa acatctatca gtttaaagta
     2401 aaagatttat atggtaaaga atttgatttt tcaactctaa aagggaagaa agtgttaatt
     2461 gtaaacactg cttccgaatg tggattgaca cctcaatata aagatctaga agccatctac
     2521 aaaaaatata gtgataagaa ttttgtaata attggttttc cagccaataa ttttggtgcg
     2581 caagaaccag gaagcaacga acaaattgct acgttctgcg agaaaaatta tggtgttacc
     2641 tttcctatga tgagcaaagt atcggtaaaa ggaaaagata tgtgtgatgt atatcaattc
     2701 cttacacaaa aaagcaaaaa cggtttgcaa gacagtgaag tagaatggaa tttccaaaaa
     2761 tacctcataa atgaaaaagg cgaactagta aaagtgcttt cgccacgcgt gttaccaacc
     2821 gaccaacaaa ttattgactg gattaactcg taattttaac taaaaagtat cgacgaacga
     2881 acacctgctc gtcatttaaa gtttttttta tcattttaac gattttaatt ctttttagca
     2941 gcattactaa atagatttgt cttgttatca tcaacgtata aacgattata aaatgcaaga
     3001 tatatttaac ccagtatata gaaaagatta ttttgaaggt ttttcagaag gtctaaaccc
     3061 aatgttatca ctaagagatt catatagtga cgcttattat gaaggatttc aattaggaag
     3121 agaagagtat gaaaacttaa acggtaagat aaccaatggc attccaaagt taattgttac
     3181 caccaaagtc ttagaagatt ttttattagc aggaatgcta ggaatgaaca tagacgaaag
     3241 cggatatacg ccataacaaa tagaaattat ccagaaatgg tatcagagtg gtgttgaaaa
     3301 gtatgatgtc tatagcaaca gaaacctttt aaatatttta gatcaaaaag gaatagattt
     3361 agtataaaaa tattatgtgt tttaataaaa acttttaagt agaagaaatt ctatttaaaa
     3421 gttttttttt acatgcattt cagaaggcta tcttttataa aaaccaattt ttttttctaa
     3481 aaattttatt tccaatataa gatcgaagcg tcaaagattt ttttcacttt tctagcttaa
     3541 tttttttaaa aaaagccaaa ataaaacctg ttccatttga aacaggttta tataaaagtc
     3601 aatttttaga ttagaattgc tctctaccag caaaatggaa agcaccttcg atagctgcat
     3661 tctcatcaga gtctgaaccg tgaacagcat tttctcccat tgaagtggca tattttttac
     3721 ggatagttcc ttctgctgca ttagctggat tagtagcacc aattaatgtt ctaaagtctt
     3781 ctactgcgtt gtctttttct aaaatagcag ctacaattgg accacgagtc ataaactcaa
     3841 ctaattcacc ataaaaaggt ctttctgagt gaactgcata aaatgctttt gcgtcagcta
     3901 cagtaagttg tgttaatttc atcgctacaa ttctgaaacc accttcagta atcatgttta
     3961 aaattccacc gatgtgtccg ttttcaacac catctggctt aatcattgta aatgttctat
     4021 ttgttgccat ttttgatttt atttttttaa tttgggtgca aaagtagtgt tttttaaacg
     4081 ataagcaaaa gctgctacaa agtaattatt gtaaagtttg tttctaacgg tttaaggttt
     4141 ttcaacaaac tttgaataag cctatcgcca gcttctaaat aaaattcaga gaaattaagt
     4201 tttcgttctt gtaaagattg gtttggaaac aattcgtttt gaagcaattt aatgcgttct
     4261 aattggtcgt gatgtattct cttttcggct tttaataatc ttttctccaa attatctaaa
     4321 ccttttgttt gtttgatttc ttgtgctttt acagcaccaa taaaggatgc atctgtagct
     4381 tctgctattt tgtataatgc ttcaaactgt ttttttagga attctttttg agcagaaaaa
     4441 tcgatagtta ctgctgaaag ttctttggtt ttactgttga ttaaatccaa ttgtttgcta
     4501 aataaatcag tccaagtaag attgagtttt ttggttttag taagttgttt ttcagtaaca
     4561 atcactacgg aattccgaat taataagata ggaaacgtga tgtcaaaagc tttaaaattt
     4621 gacttcaatt caagccagta ggcaatttcg ccaccaccgc caatataaca aaggttaggg
     4681 agaattactt cttgatacaa tggtcttaga attacgttag gactaaactt ttctggatga
     4741 ttatctaatt ctgtcaacaa ttcttccttt gaaaaagcga ttgtagtatt cagtacttta
     4801 taaatccctt tgtcgtttac aatgcgttct ctaagcgttt cttcaatata aaacaagtta
     4861 atttctctcg ggtttacttg aatgttgtag tcatttaaaa tagctgctgt ttcattaact
     4921 ttagtaaatg atgtttcttc taaaagttct tttttaacat aaggaatgaa taattttttt
     4981 aaggtagtgg catctccatc aagaatgacc aaaccttcat ttttaaataa ttcattagca
     5041 atatagcgtg tggcatcggc taaattggtg tgttccaaat atcctttttg gaacaactgt
     5101 tttaggaagt cagcgttttt gcctacgccc aattcttttt caaaaacatc aaatacttct
     5161 tctaaaccag tggttgacaa tcttccaaca ggaccaaaac tttctttatt ccatcgtatt
     5221 tttttttcat gcaattgaaa atgattgatt tcttcaaaat catggtcttc tgttgccatc
     5281 caataaatag gcacaaaatt atggtcagga tagcttgtct ttagttgttt gcaaagatta
     5341 atcaccgaaa tgattttgta taaaaaatac aacggaccag tgaataaatt caactgatgc
     5401 ccggtagtta tagtgtatgt gtttgtatcg tttaagtgtt taatattgtt taaagtaatt
     5461 tcagaaacag tacacttttg atattgcttt agcagttcat ccacaagtat agctctattt
     5521 tcaagcggaa agttgtcttt tttctcttct atttgttttt tgaaattctc tacagttgga
     5581 aaacgattgt acaattgctt caactcgggc ttctcttcta aataatcaat aattaattta
     5641 gtaaaataac cagatttttt atagctaatg cattcggatt cagtcatatt gattcttatg
     5701 ggtttgtgat aatttttcca agtatatagt catcacctaa cgaaaactga ctgtttcctt
     5761 ttttcaacga aacatttttt aaatgaatag tatgctgaaa actagttcca aaagtagtgg
     5821 tagaaacttc aatcattccg ctaacattag ttacaccttc aacggcattc cattgttggc
     5881 ttagtgttgg tgtagaagga gttggattgg tacaaaaatg atcattgtca acgacattgt
     5941 tatacaatct ataactcaaa gtgtttgttg cgtcaatcaa agctgtcctt ggagttgtag
     6001 ttacttcgtt agcaaataaa gttgcaaaat cagctggcgc aaaggttaaa acttcgcttc
     6061 cattaaaatt ataaacaaca tcggaacaag tgctttttac agcattatct tcgtcaaaac
     6121 caaatgctag tgcggcaata tttgtattgt aattaccaaa cacataagtg tcatacgttt
     6181 gaatgcctga aggtttttga aatgcgatgt ttttaaaaac aatataattt ttgtaacctg
     6241 ttatttttgt agcatttgta actgcatcgg tagtttttat agctgttgtt gtaatttcaa
     6301 tagtaccaga agtagctctc cattcttcaa taacatttgg ggtagctgac ggaacagttg
     6361 gacaaatatt gtctaatgtt acaatgtcat tatattgtct ataagtaact tttacatcgc
     6421 ccgttatagg aattgaaatt ggtgcatcga ctagtgtttc atcgttcaca aaaatcgttg
     6481 ccggaatttc aacgaataaa atctcagaat ctttaatttt ataaattaca tcttttacat
     6541 cacatttctt ggcaataata tcttcaaaat caatattatc tacttgtaaa tcgccatcat
     6601 cacaggcatt tattataaaa gccaaagcaa ttatacttag tattcttttc atcttaggaa
     6661 taattttaaa gcaaaaatag atttttttat gtcaaaataa taggttttat catttattta
     6721 ggaagcttac ttttatattt gcagtatgaa aaaaatctat ctcgataatg cagcgacaac
     6781 accaattcgt caagaagtaa ttgatgaaat ggttatagtg atgcagtccg aatatggaaa
     6841 tccatcatct acacacagta ttggtagggg ttcaaaagcc atcatagaaa gttctagaaa
     6901 aatcatagcc aagcacctaa attgttcggc acaagaaatt atcttcacag caagtgctac
     6961 tgaagctacc aattggattt tgcgctgttg tataaaagat ttaggtataa aacgaataat
     7021 tacctctaag attgagcatc atgctacact ttataccatt catgcattgc tgaaagaatt
     7081 tgacatcaag gttgattata tcaacattct ttccaatgga aatttagatt atgctcattt
     7141 tgaaagttta tttgataacg atgaaaaaac tttagtttct ttaatgcatg tcaacaacga
     7201 aattggagta attaacgata ttcaaagaat aggcgagtgg tgtcatcagc atcaagcagt
     7261 gttccattgt gacactgtgc aaagtattgg aaaaacacgt tttgatttac aaaaactacc
     7321 tgttgatttt ctagtgtcaa gtgcgcataa atttcatggg ccaaaaggag ttggatttgc
     7381 ttttctaaca aagaaaatag gattgaaacc aatgcttttt ggaggcgaac aagaaaaagg
     7441 attgcgtgcc ggaaccgaag cagtacatca aattgctggt ttatctaaag ctttagatat
     7501 gtcttttcaa aacattgata aagaagctaa ttatataaaa gaaataaagg aatattgttg
     7561 gcaacaatta aaagaaaact ttcccgaaat tcaacaaaat ggtgaaaaca ctttatacaa
     7621 cattctaaat gtcttgctcc ctttttcggc agaaaaaaca gccatgatat tatttaacct
     7681 cgatatgaaa ggcattgcag tttctcgcgg aagtgcttgc caaagtggaa gtataaaacc
     7741 atcacatgta ttggctgaaa tgttaaatga aaatgacatc aaaaaaccaa gtttgcgcat
     7801 ttcttttagt cattataata ctaaaaaaga agtagatgtt ttcatagaag aattgaaaag
     7861 aatataaaaa aataccgaaa ctaaaagttt cggttttttt tttatttaaa aggataaaac
     7921 ataaaaaaat acattttaaa ctgatttatt tatctagaaa aatttacttt gtttggtttt
     7981 tcatttcttt ttcaatcata tcgtaaaatt cgtcaatctt aggtaaaact actatacgag
     8041 ttcttctatt ttttgctcta ttttccacag tattattgtc aactaaagga atgtattctc
     8101 cacgaccagc tgctattaat tgagatggtt ttacgcctaa atcattcact aatactctaa
     8161 cgatagaagt tgagcgtttt acacttaaat cccagttgtc taataaaaca gcatttcctg
     8221 taaaaggtac attgtcagta tgaccttcaa ccatacattc aaaagtagga cggctattga
     8281 taactttagc tactttggct aaaacgcctt ttgccttatc gcttacaatg tagcttccgc
     8341 ttttaaacaa caatttatca gcaatagaaa taaataccac tcctttttct acattgattt
     8401 caatatctgg gtcagatatg cctactgaac tttttaaact tgttactaaa gccaaagtca
     8461 cactatcttt tcttgtcaaa gcatcttgaa gacgcgatat ttttaaatct ttttctttta
     8521 aactttctaa tgatttctca aggttttgag caccttttga tgttaacatc gtcatctctt
     8581 tttgagaatt aattaaatca gaagtatttt gttttaaata gtcattttgt ttagaaagcg
     8641 catctttctc agataaacat aaattcaatt tcatagtagt tgaatttagt aaatcttgac
     8701 attctttatt ttgcgcttct aaggcagcaa tttttttctt tgaaccacat gatgtcaggg
     8761 ttaatgctac taacgataaa gcaataattg atttcttcat aaaatatatt gtatttaatt
     8821 acactacaaa agtaataata aaacgttcta attgaaagtt tatggttata aactattgtt
     8881 aattttagta aataatttgg tgtttttaag aaaataacaa aaaacaatgc tttttaaata
     8941 ataataatct tgagaattat ttggttttct ctttcttaga ttcttattaa tcattttata
     9001 aaaataaaaa tgagctttca gtatagccag aaaatgtttc cattgtcctt ttactaaaaa
     9061 ttgtattcca gctattccgt ccattgataa tctataaaaa agtatggcta ttaatttatt
     9121 ttctggaaga tttttggtta acatgaagag cgaatttcta aaatttaaaa aagttttttt
     9181 agggtttcct tcttgaagtg ttgctccacc aacatgataa acaacagaat ttgaagtata
     9241 ttttgttttg aaaccaagat taaaagcacg ccaacataaa tcgatttctt cttgatgagc
     9301 aaaaaaatca tcatcaaaac catttaaagt tctataaatt tcttttcgaa taaaaaaaca
     9361 cgcacctgaa gcccaaaata tttctttttc atcatcatat tgtcccaaat cagcttcgat
     9421 agtatcaaaa attcgtcctc tgcaaaaagg aaaaccatat ttatcaataa aaccaccagc
     9481 agcaccagca tattcaaatt tagttttgtc tttaaaatct aaaattttag gttgaattat
     9541 tccaatggta ttgtcttttt caaaaagatg ttgaatagga ataagccaat tttcagttac
     9601 ttcaacatct gaatttaata aacaataaat ttcttcttca actagttgca aagcttgatt
     9661 gtatcctttt gcaaaaccat aatttgcttc attatgaata atagaaatag ttggataatt
     9721 atctttaata aattgtatag aattatcagt tgaagcatta tcggctacat aaatactggc
     9781 ttcatctgaa tgtaaaataa ctgacgacaa aaacttttct aagagttctt ctccattcca
     9841 atttaatatt acaacagcaa tttttttcaa agtttgatga gtgttttaaa gaataaaatc
     9901 aggcaattct ttgaggaaat tataacgttc attttcaaaa tccatttgac aaaagtaatg
     9961 atttagacca ttagtaacca ttaaatattc ggctttcaaa actagattgt atcttgcaat
    10021 ttgatcaaaa gtagcttgtg aaatgctaat ttcaggtgct ttgcattcaa ttaatagaaa
    10081 aatagctcca tttggtttaa aaatgacaat gtcatatcgt ttatttctat cgtttatttt
    10141 aattaatttt tcaacgttga tataagacag cggataattt ttctctttca ttaaatattc
    10201 aatcgtgtgt tgcctaaccc attcttctgg agttaaaaga ataaattttt tcctgatagg
    10261 gtcaaaaata gacactttat tttcactatt tttgaagcga aaggaataaa caggaaaatt
    10321 gagtttttgc ataaacaaaa ataatcaaat ttaaagaact gccaaccgtt ctcgaaattt
    10381 tggaatgaaa accgaatact aacaaatgga cgaaatagta aaaataatta atgatataaa
    10441 agtaggaaac atcaaaccaa tttatttttt gatgggcgaa gaatcgtatt atattgataa
    10501 acttacagaa tatttagaag ataacctttt gacggaagac gaaaaaggtt ttaatcaaat
    10561 ggtgttatat ggaagagata ctactattga agatatagtt tccaatgcaa aacgataccc
    10621 aatgatggca gaacgacaag ttattattgt taaagaagct caagatttat caagaacaat
    10681 tgataaaatt gaagcctatg taaataaccc acaacctacg actgttttag tttttgccta
    10741 taaatacgga aaagttgata aacggaaaaa atggataaaa tcaatcgcta aaaaccatat
    10801 tctttttgaa agcgaaaaat taaaagactt taaagttgcc gattggataa aaagagtttt
    10861 gcaaggcaga ggtttttcta tagaacctaa agcagccata atgctgcaag agtttttggg
    10921 aaatgacttg agtcgaatag caaacgaatt aagtaaatta gaaattattt tgccaaaagg
    10981 aagtacaata actccagatc atattgaaca aaatataggt attagcaaag attttaatgt
    11041 atatgaattg cgaaaagcaa taggcgaaaa aaatcaccac aaagcattcc aaattctaaa
    11101 ttattttcac caaaatccca aaagtattcc gaaagtattg ttgattggtc aattgtttgg
    11161 ttatttttca aatttattaa aataccacgg tttaaaagac aaaacacaag gaaacgtagc
    11221 aagtgcatta ggtattcatc ccttttttgt tgatgaatat agaattgctg cggctaattt
    11281 tccgatgaaa aaagtaagca cagttatcaa taaactcaaa gtagttgacg gaatgagtaa
    11341 aggtgttggt gcttccaatc ttgaagatat cgaattgtat aaagaatttt taatttcggt
    11401 aatgtaaatt aaagttttta ttgagtaaaa aaaatagtaa atttgctttc caaacaacac
    11461 tactatgagt caaaaaatat tgcttacttc aaaagaagtc aatatcattc tccatcgttt
    11521 ggcttgtcag ttaatcgaaa atcacaatga tttttcaaat acggtattaa ttggaattca
    11581 accccgaggc gtttttttag cagaaagaat aaaaactatt ttagaaaata gttataaagt
    11641 aaaagaagtt acgctaggtt ttttggacat tacttttttt cgcgatgatt ttcgtagagg
    11701 cgaaaaagta ttagaagcca atcaaacaaa aattgacttt ttagtagaaa acaaaaaagt
    11761 tgttttcatt gacgatgtat tgtttacagg aagaagtatc cgttcagcat taaccgctat
    11821 tcaatctttt ggaagaccat caactattga gttattggta ttaattgacc gaagatttag
    11881 tcgcgaatta cctattcaac ccgattatag aggacgacaa gtagatgtta ttaataacga
    11941 aaaagtggtt gtgaattgga gcgaaaagga cggagaagac gctatttatt taaaagaaaa
    12001 agataactaa aatcatataa attggaacta tcagttaatc atctgcttgg aattaagtac
    12061 ttaaatgcta atgatattca attaattttt gaaactgccg accattttaa agaagtcatc
    12121 aatcgtccaa taaaaaaagt tccaacgctt cgagatataa ctattgccaa tatttttttt
    12181 gaaaatagta ctcgaaccaa actttccttt gaattagcac aaaaaagatt atctgccgat
    12241 gtgattagtt tttctgctgc acaatcttcg gttaaaaaag gtgaaacact tatcgatacg
    12301 gtcaataata ttctttctat gaaggtagat atggtcgtaa tgcgtcatgc gaatcctggt
    12361 gctgcctatt ttctttcgca aaatgtaaaa gcaagtatta taaatgctgg cgatggcgca
    12421 cacgaacacc caacccaagc tttgctagac agtttttcta tacgggaaaa acttggtgaa
    12481 gtagcaggaa aaaaagtagt tattgttggg gatgtattgc attcacgtgt ggcactatcc
    12541 aatatttatg ctttgcaaat gcaaggtgcc gaggtgaaag tttgtggtcc aaaaactctg
    12601 attccaaaat atattgaaag tcttggtgta acagtagaga caaatctaag aaaagcactc
    12661 gaatggtgtg atgtggctaa tatgcttcgt gttcaaaacg aacgaatgga agtcaactat
    12721 tttccatcaa caagagaata cgcacagcaa tatggtgttg acaaagcatt gctcgattct
    12781 ttaaataaag aaattgtaat catgcatcca ggtccaataa atcgtggtgt agaaattact
    12841 tcggatgtgg cagattctaa acaatccgtg attttaaatc aagttgaaaa tggagttgcc
    12901 attcggatgg ctattattta tctattggct tcaaaaatac aataaactaa aaaatcctct
    12961 tttcaaaaga ggatttttta gtttttatac ttactcagcc ataaaattat taagttgttt
    13021 tagcttttct ttccaagtag ccaattcttc tttttgcttt tcaatgtttt tgtgaacttc
    13081 ttttaccaaa ggattgttag catcagcatt tgcaaaaaac tgtatgttat tttctaattg
    13141 gaaaatgtca ttctgaactt catcaatttt gcgcatgatg aatatttttt cgccatcaat
    13201 ttttctagaa tcaagactag ccagatgatc tagtttgtta gaatagcgtg caatatcgtt
    13261 atctttttta cttgaactta atttttcaaa taaaacgtct aatattttat taaactttcc
    13321 ttcaatatag cgtcttgaaa aaggaacttt tccaaagctt ttccatgttt caatatgcgc
    13381 ttttatagcg tctaaatcgg ttttatgttc tccaaccaat tcaaaagatt taatcgtttc
    13441 taaatattct tttttctttt caaaagcttc aatttcttca gaatcaagtt cttttcgttg
    13501 tgattttaaa ttttcgaaat aagcattgca agccgctcta aattctttcc aaagtttgtc
    13561 tgataatttt ctgggaacat gtccaacaga cttccattct tcttgaattt gtttcatcaa
    13621 tggagtggta gcttcaaaat cagtattgtc ttttaatgct cttgctttgt ccactaaggc
    13681 ttgtttctta gctaaatttt cgttttgatc ttgttttaaa tctttataaa aagaattttt
    13741 tagtttgttg aaatttctaa cggccgtttt aaaagcaaac catgtagctt catttacgtc
    13801 acttggtact tttccagcgg caaagaattg attgcgcaat gcttccactt tatggatttg
    13861 ttcttgccaa gcattgtgag tagttacttt ctcagcagct aacacttcca attgagagat
    13921 aatctctttt ttgatggcta gatttgcttc ttcaacttct ctaaaactag cataaaaact
    13981 ctcacgtttg tcatgcattt gcttggttaa atcactaaac tgattccaaa tatcttctct
    14041 tacctctctt gaaactggac caatttcttc tttccaaatt ctgtgtaaat cttgtaattc
    14101 tctaaatgcg ctgtgaatat cttcttcttg taataattct tcaacgcgag caataatttt
    14161 ttgcttttgt tctagattat gcttaaagtc taaatctcta atttctctat ctaggtgcag
    14221 aatatcataa aaattctcta aatgaaaatg ataattattc caaacatgat tgtatttatc
    14281 tttcggaatt ggacctgcta ctttccatcg atctctaatg tcgttaaatt gctttaacat
    14341 cgttgacata gtttcggtgc tgttgattaa ttcttttaat tcttcaacta tagccaatct
    14401 tgtatttaaa tttgctttta agttgttttg aagcgattga aaatgattgt ttttcttatc
    14461 tctatattga gaatataagt ggtcaaaggt tgattttaaa ggtaaatgat aatggaaatc
    14521 ttcggtagtt tctgggtttt cagcaagaaa ctcttctttt ttctcttcga tgaaatggtg
    14581 gtactcggat aagaaggctt tcttaagttc ttcaacatgg tctttaaccg acattacttt
    14641 ttctacggca acaagttttt caagttcttc caccaattgt tgcaacgaca tcgtgtcata
    14701 atccaacatc gggatagcat gacgttcttt tatagtttcg tcttcacttt cttcggcatt
    14761 gctgtcttca atagtttcaa ctgcagattt gatctttgtt tctactacaa aagtatcatc
    14821 ttccagttgt tcgtctgtaa gttcaataac gttttcgtta acttcatttg gttgcgattc
    14881 ggtaataatt tcttcagaag cagtatctag tgttgtttct tctaatagta cttcttgagt
    14941 ttcaatttca tccgctacaa cttcttccga agtggcttca actattgtta ttatttcggc
    15001 atcgttgttt tcttcttgaa ccgattgagt tggttcatcg attaggtttc catctgcttt
    15061 aggcaggtta tcattctgtt cttctaacat ttttaaaatg ttttaaatta tacattaata
    15121 gggcgaaaga tagtaaacaa ctatcaatct acaaagaatt taaaaggaat atgaactttt
    15181 gtgtccgaat ttctatagca taaaaaaacc cagtattact actgagtttt aatgtagtat
    15241 ttaaaaaaaa caattaattt acaattactt ttttggttat tctttgatta ttagagtcaa
    15301 tagtgatgaa atagaagcct ttgctaaggt ttgaaacttc atattgatta tcaaaaacag
    15361 ctgggttttt gaaatattgg attacttgtc cattcacatt taaaacagaa atcgcatcta
    15421 tagcaaaaga acccgtagtt attgtgaatc taccatcatt agaaggattt ggagaaatag
    15481 ttactgtagg ttcaagtaaa tattggtctg tatttaaaag actaccccaa atttgcatgg
    15541 catattctgg atggtcaata taagggttac gattgttttg tctagcataa atagcattgt
    15601 ttcggtcaat ttctctttga ctcacaggat cgtcgttgct ccatgttaat aatacattta
    15661 tagcttgatt agtaaatact tgattgctag tattgttaaa catagcaaag ttccaattgg
    15721 ctacttggtc ttcatatctt gtagcaaaat acaaaagcat tctagcgatg tcgcctttaa
    15781 attcattaat aggttcaaat actgttccgt tgtatccgtt tgaatatcct gagtttaagt
    15841 tgtttccttt ttttgatcca ttagaataag tattggtaac agcatttact ctactaaacg
    15901 gtaaatctcc acgttgtgca tttacatatt tatccgatgg tactacaaag tgggcatcag
    15961 aatacattgg tgtaacacca cttccaaaaa ctgattgtgg aataagatgt tctctgttgt
    16021 aacgttcgca ttctgcattc cctagagtac catcatcttg gttgatacca taggtaaact
    16081 cacattcggc acccgttggg ttttcggtgt acatatctaa cattgtacca tcgttttcat
    16141 aatagaaatc tttatctgaa gttgtataag tcacatacaa accagaataa cctctatcat
    16201 tgtgattttt gataatgttg tgcaattgtg ttttaagtat ataacctgtt cctgttgctg
    16261 agttgtaata tccatttggg atttgcgcaa aacaaataga aactaataac aataatagtg
    16321 tggtaaaatt ctttttcatg gttaattgtt tttttgaagt aatgccatat agaatccatc
    16381 aaaacccgat tctgaggcta atatttttac atcttttaca aaggtaaatt cttttccgtt
    16441 ttctgtggtt aaaaaacgct taatttgttc atcgttttca gaaggcagga tggagcaagt
    16501 agcatacacc aattgaccgc ctggttttac aattcttgaa tagctttcta agacttcggc
    16561 ttgtactttc ttgatgttgt ctataaattc cggctgcaat ttccatttgc tgtcaggatt
    16621 tcttttgagc acacctaatc cgctgcatgg tgcgtcgatc agtactctat ccgctttttc
    16681 atatagcttt ttgataactt tggtgctgtc tatgattcgg tattctatat tgaaagctcc
    16741 gtttctttta gccctaattt ttaattgctt aagtttactt tcatataaat ccatagcaat
    16801 aagttgtcct ttgttctcca ttaaagaggc tatgtgcaac gtttttccac cagcaccagc
    16861 acaagtatct acaacacgca taccaggttc aacacccaag aatggtgcta ctagttgaga
    16921 tgaagcatcc tgtacttcaa agaaaccttg cttgaaggca tcggtcaaga atacattggc
    16981 gcgttctttt aaaactaaag tatcgggttg attttttaga tactctgtgt caatattcaa
    17041 atccattagg atggcacgga gttgttctct attggttttg agtgtgttta ctctcaagat
    17101 tactttggct tgttggtttt gtgctgccaa ttctttagtc cattgctctt ctccaagctc
    17161 ttttacaccc aattcatcca tccaatcggg tatagattct ctatattttc taattttgga
    17221 tagttcgtca aagcgacctt taatttttct ttgtggtgtg ccttccagtt gtctccagtc
    17281 tggaataggg tatccacgaa gtactgccca aacagcaaac aatcgccaca aatcttctct
    17341 agtaaaaggc tcttttactt cagctatttc ggcataaagt cttttccaac gtactatctc
    17401 atagatagtt tctgcaacaa actttctgtc cgagcttccc caacgtttgt ctttcttcaa
    17461 agcacgagca actactttat cagcatattc accttcgtta aagatggcat ttaaggaatc
    17521 aatggtggtg taaactaagt ttctatgtaa tcgcatgggt attaaaaata agcggacaaa
    17581 gatatttctt ttttatttgc taatcctact ttgatagtgt taaaatatgt attaagagca
    17641 attaataaag gaagaattaa tctattctaa tcatctctat gttttctgga gcatgcaaaa
    17701 ccattgggaa tttttctact ttgactgagc tgctatccaa cccaaatcct ttttgttctg
    17761 ataaacttag tttttttaag ataaaatagg tgttcattac tattttttca aagaaagtta
    17821 ggtggctatc gttggataag aatttttcgg aaagtacaaa tttaaagtca ccaataatgt
    17881 tgtttttgtt tagagattcg tatctacttg aaatgtccat ttcgcctctg gctaccattt
    17941 ctttaattac ttctttaaac attaaattta tcttggtagc ttctctaaaa ccaaggtaaa
    18001 agtcaactct ataaaggtct tccgaaaaga tttcggttac tttgtattcc tttcgataag
    18061 gttcgttcaa aatgtttaca tgtacaaacc aatatatatc agcacgtttt ggtctttttt
    18121 gaagaataga atacattact ttttcttcaa tttcgtccga gcgttgcgca ttagtcatat
    18181 agaccaaatg ggttgcatat tttggaatgg ataaatcgac acttagttct gccaagacct
    18241 ttttgtaatc ctctatttta acaaagtttg tatagttttt acttatttgt ttggcaagat
    18301 accaagtagc cataatgcca attaatgcaa aagcgattat taatgttaca aatccgccgt
    18361 gtagaaactt tgtaatgttg gcatatagaa agctaaattc aataaccaag taaagactaa
    18421 tcaatggaac gatgaaataa agctttacac gtttcatgat taaataaaaa ttaagtaaga
    18481 atgttgtcat aatcatgcat agcacaatag caagaccata tgcatgttcc atattacttg
    18541 actctttgaa gtaaagaaca atacaaatac aacccaagag taacagccag ttgatggaag
    18601 gtatgtaaag ttgtccttta acttcagttg ggtattttat ttttactttt ggccaaaaat
    18661 tcaaacgcat tgcttcattt attaatgtaa acgaaccact aattaaagct tgtgatgcaa
    18721 ttactgctgc cattgtagcg atgataattc ctgcaggttg aaaccaattt gccatgatta
    18781 gataaaacgg attaccagca ttgccaccca attctgttaa agttttccct tcatggttga
    18841 ttagaaaagc accttgacca aaataattta aaagaagcat tgtctttaca aatatccaac
    18901 tgatacgaat gttttttctg ccacaatgtc ccatgtctga atataaagcc tcagcacccg
    18961 ttgtacatag gaaaaccgca cccaaaacaa aaaagccttc tggatgcaaa gttaacaatt
    19021 cgtaggcata ataaggattg acagcattta atacagacac atcttgcacc acttgaagga
    19081 tgcccaaaac acccaacatg gtaaaccaaa caagcatcat aggagcaaaa aacttgccca
    19141 ctatcttagt gccaaattgt tgaatagtaa acagaatgat tagaatagta attactattg
    19201 gtatagtgtt tatttctgga taaaaaaggc gcaaaccttc aactgccgaa gaaaccgaaa
    19261 tgggtggcgt gatgatacca tcggcaagca aggcactacc accaatgatg gcaggtacaa
    19321 taagccattt tatcttcatt ttttttacta gagcaaaaag agcaaaaatt ccaccttcac
    19381 catgattgtc agcacttaga gtgatgataa cgtattttag agtagtttga agcgttagtg
    19441 tccaaaatat gcaagatatt ccgccaagta caatatctct agagattgga tagtcgccta
    19501 aaatagcttt cattacatac aatggagatg taccaatatc gccataaatg attcctaatg
    19561 tgattaataa accagctgta gaaagcttgg aatgcaagtt aagatgtttg aaggaaccca
    19621 tgaaagacaa ttagttaaag agtgcaaatt tatataaaat agatagaata gggtagaata
    19681 agggtaaatt tttattgtgt caattatttc aaaatatttg aaacaatatg attctttaac
    19741 tattgaatac taatgagttg tctctagttt ctacaatctt aaaaactatt aagtagtacc
    19801 ttttcaattt gcagaactct tttttttaac taattacagt ctaaattctt cgtgtacacc
    19861 taatccgaat agtgcaaagt catacttaac aggatcatct gaatcgaatt ctcttagttt
    19921 tttatcaagc aggtttactg ttttaatatc gtcttgtctt ctgtcgatta gtcctaagct
    19981 tctagctaca tttccagtat gcacatcaag tgggcaggat agttttgaca ttggtatgtt
    20041 tttccacagt ccaaaatcaa caccattttt atcatgacgc accatccaac gaagaaacat
    20101 gtgcattctt tttgctgctg ctccagttga aggatcggca aggtgttttc gagttcttat
    20161 ccaatgctca atttcaaaaa aaatagttcg aaagttgact atgctttcgt gcatttgagt
    20221 attactttga tttaaggtga aaatagtttc caatccgcta tggtttgtat aaatattatg
    20281 aagcgatttg atataaaact gacaatcaat accattaaat gttctgtgtg tgaagtcgct
    20341 tagcttttcg atatcacttt ctgaatgatt gagaataaaa tcatatggcg aattgcccat
    20401 taattccatc aatttttttc ctttattgat gatagttttt cgttgtcccc aagctaaagt
    20461 agctacaaga aaaccagcta tttcaatatc ttcttttaca ctaaactgat gaggaatctg
    20521 aattggatca gtatcaataa aagacctgtt gttatattga acaacttttt catctaaaaa
    20581 ctcttttaac acttttccgt ctag
//
LOCUS       DAOOBH010000027        20593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_3422_length_20593_cov_6.229136, whole genome shotgun sequence.
ACCESSION   DAOOBH010000027 DAOOBH010000000
VERSION     DAOOBH010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 20593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20593
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3422_length_20593_cov_6.229136"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2486)
                     /locus_tag="PK218_04740"
     CDS             complement(<1..2486)
                     /locus_tag="PK218_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97847.1"
                     /translation="MKKQLLYFALLYTAFSFAQVGINTTTPDASSMLDISATDKGVLV
                     PRISLTNVTLTTLDGTNTAATGLLIWNTNATTVGGNGVGFYFFNGTQWMPITQTATVN
                     NLDQAYDQGGAGAGKNIDATDGAVRINGTDGFLVTGISGNGNTIDIELTGAGTRMFFN
                     PRKSAFRAGRIESSQWDNDNIGSLSTAFGYNTTASGSNSTSFGNNNIASGQGSTAFGV
                     STEANAIYSTAFGSQSTASGSTATAFGFNTIASGSQSTAFGGLSTASGTRSSAFGSSA
                     FASGDYSTAFGYSTVASGNGSTAFGFDTDAIGNYSTAFGYNTEAIGNYSTAIGYGNEA
                     RSFGETIIGIGATFYSVSTNGNTEFKTANQNDRLFVIGNAIDLNENGTFDGTEASNAL
                     TIYKNGRMNINDAYNMPLTDGTSNQIMRTDGIGNVSWVTPTVGTDDQNLTTPSLTGTT
                     LNLGIENGTGTSINLSSLVGTDDQNLLTPTLTGTTLNLNIENGTGTSIDLASLSNDWK
                     LTGNSGTDSSINFIGTTDNQDLVFKRNNGYSGAIRSSNTSLGLNTLNQATTGDSNAAF
                     GVFTLTSNTDGESNSALGYNVLRNNTSGSFNVAIGRSALFANTIGVENTALGNNSNSL
                     NTTSSGNIAVGFDALASQSFNNGGSAWYSYNTAVGYASLRSNQPSASTNAIQNTAVGA
                     FTLNNNLRGKNNTSLGFEALRNNRNSSQNVAIGSRALFTQSFPNGGSEWESNNVAIGF
                     ESLFSNQPTNATNAINNTAIGTQSSYSNTTGIGNTAIGYRALYTNTNSSNNVAIGINA
                     LTLNTSTSNVAVGAYSLSTNVGGTENTAVG"
     gene            complement(2483..2704)
                     /locus_tag="PK218_04745"
     CDS             complement(2483..2704)
                     /locus_tag="PK218_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97848.1"
                     /translation="MANINEPIIILETDTNGYYLIKILDKIQRKIVFSDTLKSENKHI
                     AIKGFTPNLFEITIEPVLLNNRWEKILLL"
     gene            complement(2812..3525)
                     /locus_tag="PK218_04750"
     CDS             complement(2812..3525)
                     /locus_tag="PK218_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW97849.1"
                     /translation="MPTKIYILEDEPLIAETIKTCLEKEGYIICGMADNVKEAIFDVE
                     QMQPDLVLVDITLDGDLTGIDFVNFLKKKFDIPFIFLTSLSDSKTLEKVKETEPFGYI
                     VKPFNEKTLKTNIELALHKHQIHKQSVSVAYESDSFFIKNKGELIRIVLDDLLYIEAF
                     DNYCYVITATQKLLISYTLKNVEDKLPQPQFLRVHRSFCVNTSKITSLQEGYLFIEKN
                     KIPVGKSYRDQLMQHIRLL"
     gene            complement(3532..5274)
                     /locus_tag="PK218_04755"
     CDS             complement(3532..5274)
                     /locus_tag="PK218_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPW97850.1"
                     /translation="MIKQKKIFSLFFLIIGIGIFSQKKQPDFSKDPSYPEFKTYLDKI
                     DSLIDTENDSIYIYIDKANLLAKKMNYKLGEVKVQEAKGSYELYVKNNYNGAQVIFLK
                     CIEKCEKYKLNYLGNIYHSLGKVFHMSDNYKKAKQYYNISYDYAQKEKDTAAIVLSLS
                     SIGTINYREGNYVEAEKYFQKSLSYPSSNTIKNIIFSNLCNMKIREEKFDEAENYAVK
                     AIQYNPIKGGDAIDYSRLLDAKNGLKSYVGIDTILKNSKQLFENSTSLRDKTIILASI
                     SITHYNLGQYQKAADDKDQYIDLFYDLKAQERDDVVYEMEAKYQTQKKEEEIKKQIEQ
                     KEKLYYYIGISLLLILILSYLVFLNIKQKKKLNTQKNQLQLLVDEKNVLLKETHHRVK
                     NSFQIVSSLLYLQSENIVDKEAAMAVKEAQNRVKSMILIHQKLYSKDQLIGIDSEEYL
                     VDLVDDIIQNQSFSIENLLVTKNCESYVFSIDTITPMGLIVNELITNVIKYAFPEKHP
                     NPELMVSFMKKNNGLELQVADNGTGIKNQENKNSFGLKLVNSLAKKLKATVTFENKNG
                     TTITLFITKFEELQ"
     gene            5447..5983
                     /locus_tag="PK218_04760"
     CDS             5447..5983
                     /locus_tag="PK218_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HPW97851.1"
                     /translation="MSTDSKKIANSEKSKVAKLQDIVAKTIEQEKLITENLLHPPEEV
                     LTKGQKISDKVASFGGSWAFISSFFIILVAWIIFNTMSSKNLEFDPYPFILMNLILSC
                     IAALQAPIIMMSQNRQEEKDRKRSENDYLVNLKSELEIKALHEKMDLLLEEQIKILFE
                     TQAKQLKILESLQKENKK"
     gene            5980..6654
                     /locus_tag="PK218_04765"
     CDS             5980..6654
                     /locus_tag="PK218_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW97852.1"
                     /translation="MNVLIVEDFKELADELAEFLCKNGYLCKIVSTCAAASEEVEVNE
                     YDAMLLDLGLPDGTGFEILKTVRILKLKMAVIIITARGELNDKIEGLDLGADDYLTKP
                     FALTELGARLFAVIRRIHGFAKNELEIHGFKIALSDYKVSFNENTIALTKKEFNIFQY
                     LVLNKNRVITRLQLTEHVWGDVLEVNSDSNFIDVHVRNLRKKLEKYKTLSWFETVRNV
                     GYRINE"
     gene            6655..7977
                     /locus_tag="PK218_04770"
     CDS             6655..7977
                     /locus_tag="PK218_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW97853.1"
                     /translation="MKIKHQLAVFNVLIRLILIFVLWFFLPKIIEKVVYNHIDKSLFE
                     KEKTFINRLNKQEINDFIRRNDASETYASFSTLHSEFIQLYQSNKKLDNQQNYIINEA
                     RIIESEESNYRVLYYHFNYANANYILEIGNNLSELEDIIRALRFSVLMLLLIVVSVTF
                     FADILLVEYLFKPFNKIIQTKIKFANEPDNFNFEKIKSHSSDFDILDTGLNQMMIRIN
                     ELFQKERQFISNVSHELLTPISVLKNRFENLLQNDTINEEVEDKIVSSLRNLDMMKKI
                     IHNLLLISRIENNQYQLEDYVSIKQLINEIIKDFEDRIEEKNIEIFTNYNEDIVIKGN
                     KILLHILLFNLILNALKYNFVNGKIEIVSYEENNQFYLSISNTGHGIAKDQLDTIFNR
                     FSRNSNDQEGQGIGLAMAKSIAKVHAIEIQVASSIDDKTTFTLLFPKQ"
     gene            8233..8442
                     /locus_tag="PK218_04775"
     CDS             8233..8442
                     /locus_tag="PK218_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97854.1"
                     /translation="MKKVVMLVAILFGTSVMVNATTPKKQEVKQEAKKEVKHAKKAVE
                     AKKVAVKTEAKTEMAKPTMEAKEKK"
     gene            8589..9821
                     /locus_tag="PK218_04780"
     CDS             8589..9821
                     /locus_tag="PK218_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW97855.1"
                     /translation="MFRWLFVFLLVAIVEIYAFQAFRTLTKVRSYQFVYIILNLVVII
                     YIVNGFFHFDRSIGQTKQSLFTIGLLLLTLVPKLLITFVMIVEDIYRVFSGTITRFLG
                     DNDYGSFLPERRKFVSQIAVGLAAIPFVSFLYGMTIGKYNFKIIKQTLTFPDLPDNFD
                     GFVLAQISDIHSGSFDNPEKIQYAINLINKQEFDVLLFTGDIVNTDATEMHPWVEMFK
                     GLKNPTYGKYSILGNHDYGTYKNWPSDKALEDNFEAVKDLHRQIDFRLMLDEHVKIKK
                     DNQEIALVGVENWGKNFGEKGDINKASENLTKEDFKILLSHDPTHWEYVVKNHEKHFQ
                     LTLAGHTHGFQFGIEIPGWLKWSPVQYVYKQWAGLYENLGRYIYVNRGFGFHAYPGRV
                     GIWPEITIIELKKDKNVA"
     gene            9894..10190
                     /locus_tag="PK218_04785"
     CDS             9894..10190
                     /locus_tag="PK218_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPW97856.1"
                     /translation="MSKFGELINAQVPVLIDFYTEWNDSSVSMHPVIRDVAAALGDKA
                     KVIKIDVDKNQELAEALRIKGLPTLMIYKEGQMVWRQSGELDANTLINIVQDQL"
     gene            complement(10187..10843)
                     /locus_tag="PK218_04790"
     CDS             complement(10187..10843)
                     /locus_tag="PK218_04790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPW97857.1"
                     /translation="MSYWAKSRIWMKWLFPNHIWKIKTTEKKVFLTFDDGPTPEITNW
                     VLAQLKSFNAKASFFCIGDNIQKFPEIVSQILKHNHSVGNHTFNHLNGWKTETEKYVE
                     NTELCQKELEDGRWKKEDLQTKTYRLFRPPYGKIKPSQSKKLRKMGYKIVMWDIISYD
                     FDQTITKEKCLENVLKNIENGSIIVFHDSQKSWKNLEYVLPKTLEFLKEKGFSSEKII
                     "
     gene            complement(10921..14199)
                     /locus_tag="PK218_04795"
     CDS             complement(10921..14199)
                     /locus_tag="PK218_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPW97858.1"
                     /translation="MQHFNFNKWNTILGWSTFAIALITFTLTVEPTMSFWDCGEYIST
                     SAKLEVGHPPGAPLFQILGAFFAMFATSKETIALMVNMLSVFSSAFTILFMFWSSSII
                     LRKLVSQFSEVDKSKSIVILGSSFVGALAYTFSDSFWYNAVEAEVYAMASLFIALLFW
                     LGLRWEEDMNTPKGNRWLLLISLVIGLSFGVHFMALLTIPSIGFLYFFKNYKKVTVKN
                     FVLANIIIVAILLFIFKLLLPYTLAFFGKTEIFMVNNFGLPFNSGTIFAVLLILGFFY
                     FGLKYTREKGHPFYNTIILCILFVLLGFSTWMMLPIRANADTPINENKPSDAAEVLAY
                     YNREQYGVNPLFYGPQYTDTFAGLDPDEPYLDKAPNYERDYKTGKYVIVNNWKNADQN
                     TNNQHKAILPRLWSTDHIENYMIFTSPPKFDLNYDYPFEEDLAQYGVNLDSLSSDQIS
                     QATEQLKDELRSSIREFKEAYAHKKVDNEDYVNFLKKYGDYLIVEKPSTFDNLWFMFE
                     YQFGYMYGRYLLWNFVGRQNDIQGKYDNLDGNWMSGIKVIDELHLGKGIDSNLTDDIK
                     NNKGRNFYYFLPFLLGIIGLLYHANKDLKSFYVLLALFLFTGLALKIYLNERPFEPRE
                     RDYALVGSFYVFAIWIGFGVYAIYEKTSQYLKSKLIGPVIVGICLLAAPILIASQNWD
                     DHDRSGKYTAIAMAKNYLESCDQNAILFTIGDNDTFPLWYAQEIENVRPDIKIVNTQL
                     FMTDWYIDQMKRKTYTSDGLPISFTHDEYVGDKLDYAVRIVKTEARWELKDMLNFIKL
                     PESTIDLPNGQKVHFYPTNKFRITIDKENIIKNKVVNPKDYDSIVPYIDLDIKDRALY
                     KNRIMMLDVIANNNWKRPVYFTGGSFGDDDYLWMKDYLQLDGLVYKLVPIKTKIDREY
                     SIEMGKIDSDKMYDLVTKWEWGNGDKTTIYHDTETRKNSISYRTNMSRLVEQLIKEGK
                     NEKAKKIIDEAITKMPIDYYGYYTMVEPFAGEYYKLGEKEKARELLEKLMTKYKQSLT
                     YFSGIQPSIQNGIATDIITDIERYRSLLITMKENGDDAFYNKNKETFNAFNKRFTRFG
                     REME"
     gene            14421..14491
                     /locus_tag="PK218_04800"
     tRNA            14421..14491
                     /locus_tag="PK218_04800"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14453..14455,aa:Gln,seq:ttg)
     gene            complement(14545..15369)
                     /locus_tag="PK218_04805"
     CDS             complement(14545..15369)
                     /locus_tag="PK218_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPW97859.1"
                     /translation="MKKILVPTDFSDHAHYALKVAAEIAKKNNGEVILLHMLELPSQA
                     GDAIGNGHELPEIMLFKNAAINKLEELMDDPCLDGINVSEVIQFELAFDGIMNISKKN
                     NVDLVVMGSHGASGFKEMFIGSNAEKVVRNSEIPVLIIKKDSANFNIDKFVFASDFTE
                     EIRKPFEKVVQFANEFDAELHLVMINTPSNFKPTHVSHQIMDEFIAKFSIRKFSKHIY
                     NDTNVENGILNFANHINADLIGMSTHGRKGLAHFFNGSISEDLVNHAVRPVVTFKI"
     gene            15536..16003
                     /gene="rimP"
                     /locus_tag="PK218_04810"
     CDS             15536..16003
                     /gene="rimP"
                     /locus_tag="PK218_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044047587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HPW97860.1"
                     /translation="MTFKEKVSEQIENALKVNPSLFLVDLKISEAFKISIAIDGDNGV
                     NLQDCIDVSRFIENNLDREEQDFSLEVASAGVSTPLKSFRQYKKNIGRTLKVKTLANE
                     TLEAKLEDVNDDFITLSWSSREPKKIGKGKETVQNNREISYSDIKEAIVTIIF"
     gene            16017..17258
                     /gene="nusA"
                     /locus_tag="PK218_04815"
     CDS             16017..17258
                     /gene="nusA"
                     /locus_tag="PK218_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008638810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPW97861.1"
                     /translation="MENIALIDSFSEFKDDKLIDRVTLMAILEDVFRNALKKKYGSDD
                     NFDIIINPDKGDMEIWRRRIVVADDDLDLENEEITLTEARKIEPDFEIGEEVSEEVKL
                     IDLGRRSILALRQNLISKIHEHDSTNLYKQFKDLIGEIYTAEVHHVRPRVVILVDDEG
                     NEIVLPKEKQIPSDFFRKGDNVRGIIENVELKGNKPQIIMSRTADKFLEKLFEQEIPE
                     VFDGLINIKNVVRIPGEKAKVAVDSYDDRIDPVGACVGMKGSRIHGIVRELGNENIDV
                     INYTANIQLYITRALSPAKVSSVKINEETKRAEVFLKLDEVSKAIGRGGHNIRLAGQL
                     TGYELDVIREGSEMENDDVELSEFSDEIDGWIIDEFAKIGLDTAKSILSQNVDDLVRR
                     TDLEEETILEVIKILKEEFED"
     gene            17299..20040
                     /gene="infB"
                     /locus_tag="PK218_04820"
     CDS             17299..20040
                     /gene="infB"
                     /locus_tag="PK218_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPW97862.1"
                     /translation="MSEGNIRINKVLRELNISLERAVDYLKDKGVVIEASPNTKISDD
                     VYNVLCGEFAGDKGKKEASMGISEEIRKEKEAIRLEREKEIEDKRKLDEERQQQELIK
                     AKAVVSAPKQVGKIDLEKKPASTSVPKISADKKEETPKFEKATPVKKSTEVELEKPKS
                     KVAKNETAEENVETPAEEAIETKYQKLSGTKLTGQVIDLSQFNKPKKKKEEPKKDGKP
                     NVPGTNSGNNANKNKRKRIPTKPGEGRAITPGNTNSKFGGKPGGGFQKGTRPAIVQKV
                     EPTEEEVKNQIKETLERLQGKKGGKSKAAKYRRDKRDTHRQRTEDELQAQEEGSKTLK
                     VTEFVTVGEIATMMDVPITKVIGTCMTLGIMVTMNQRLDAETLSIVADEFGYEVEFIT
                     TDIEESIIQVEDKPEDLVSRAPIVTVMGHVDHGKTSLLDYIRKENVIAGESGGITQHI
                     GAYAVTLDNGQKITFLDTPGHEAFTAMRARGAQVTDIAIIVAAADDDIMPQTKEAISH
                     AQAANVPMIFAINKVDKPAANPEKIKEQLAGMNLLVEDWGGKYQSHDISAKIGTGVKD
                     LLEKVLLEAEILDLKANPKKPAIGTVIEAQLDKGRGYVTTILVQAGTLKVGDYVLAGK
                     HHGKVKAMYDERGHSISIASPSTPVSVLGLDGAATAGDKFTVFEDEKEAKQIAAKRTQ
                     LMREQSARTTKHTTLAELGRRIALGQFKELNIIIKGDVDGSVEALADSFSKLSTEEIE
                     IKIIHKGVGAITESDVMLASASDAIIIGFNVRPAGNAKQIAEKEEIDIRNYSIIYDAI
                     DDLKDAMEGMLSPELKEEITGTAEIRELFKVSKVGTIAGCMVTDGKIFRSSRIRIIRD
                     GVVIHTGELTALKRFKDDVKEVSKGYDCGMQIKNYNNIEQLDIIEAYQEVEVKKKLK"
     gene            complement(20208..20543)
                     /locus_tag="PK218_04825"
     CDS             complement(20208..20543)
                     /locus_tag="PK218_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPW97863.1"
                     /translation="MSLKSYSQEGIVSISQDPKFEQLLNEKRKINSSITITDRYKIQI
                     YNGDNESSKKQLMAFKKEFGTLDCTIVFSTPLYKVWVGNFKTRIEAERNLIEIKKKFP
                     KSFLVKPNK"
BASE COUNT         7110 a         3075 c         3475 g         6933 t
ORIGIN      
        1 ctaccaacag ctgtgttttc agttccacca acattagtag aaagtgaata ggctccgacg
       61 gctacattac ttgttgatgt atttaaggtt aaagcattta ttcctattgc cacattgtta
      121 gaagaattag tgttagtgta taaagctcta tatcctatgg ctgtgtttcc aattccagtt
      181 gtgtttgagt aggaactttg tgttccaatg gctgtgttgt taatagcatt tgtcgcatta
      241 gtaggttgat tagaaaataa cgattcgaaa ccgattgcta cattattgct ttcccattcg
      301 cttccaccat ttgggaatga ttgagtgaaa agtgctctac tgcctattgc tacattttga
      361 cttgaatttc ggttattccg aagtgcttca aatcctaacg aagtattgtt ttttcctctt
      421 aaattattgt tgagagtaaa agcacctaca gcagtgtttt gaatggcatt ggtgctagca
      481 cttggttgat tggagcgtaa actggcatag ccaacagcgg tattataact gtaccaagct
      541 gacccaccat tattaaatga ttgagatgct aatgcatcaa atcctacggc tatatttcca
      601 ctagaagttg tatttagtga atttgaatta tttcctaatg ccgtattttc aactccaata
      661 gtatttgcaa acaaggcaga acgcccaata gcgacgttaa atgaaccaga ggtattattt
      721 cgtagaacat tatatcctaa ggcactattg ctttctccat ctgtgtttga tgtaagcgta
      781 aacactccaa aagcggcatt agaatctcct gttgtagctt gattcagcgt gttaagtcca
      841 agtgatgtgt tacttgaacg aatagctcca gaataaccat tatttctttt aaaaaccaag
      901 tcttgattgt cggttgtgcc aataaaattt attgatgaat ctgttccgga atttccagtt
      961 aatttccagt catttgataa agaagctaaa tcaattgaag ttccagttcc gttttctata
     1021 tttaaattta aagttgtacc agttaatgtt ggagttaaaa gattttggtc gtcagttcca
     1081 actaaagaag ataaattaat tgaagttcct gttccatttt caattcctaa attcaaagtt
     1141 gtaccagtta atgatggagt tgtaagattt tgatcatcag ttccaactgt tggagtaacc
     1201 caagaaacat ttccaatacc gtctgttctc attatttgat tagaagttcc atccgttaat
     1261 ggcatattat aagcgtcatt aatattcatt cttccatttt tgtatatagt aagtgcatta
     1321 cttgcttcgg taccgtcgaa tgttccattt tcattcaaat caatagcatt gcctatgaca
     1381 aaaaggcgat cattttgatt tgcagtttta aattctgtat ttccgtttgt tgaaacagaa
     1441 taaaaggtag caccaattcc gattatggtt tcaccaaaac ttcttgcttc attaccatat
     1501 ccaatagctg ttgaatagtt accaatggct tctgtgttgt atccaaaagc tgttgaatag
     1561 ttgccaattg catctgtgtc aaatccaaaa gcggttgaac cattaccact tgccactgta
     1621 gaatatccaa aagcagtgga gtagtctcca cttgcaaatg cagaactccc aaaagcagat
     1681 gatctagtac cactagcggt tgatagacct ccaaaagcag ttgattgact tccgctagca
     1741 attgtattaa atccaaatgc tgttgctgta ctaccactag cagttgattg acttccaaaa
     1801 gcagttgaat aaatagcatt tgcttcggta ctaactccaa aagcagttga accttgaccg
     1861 cttgctatat tattatttcc aaaagaagtt gaattacttc cactagcagt agtgttataa
     1921 ccaaatgcag ttgatagact tccaatatta tcattatccc attgtgacga ctcaattctc
     1981 ccagctctaa atgcactttt tcttggatta aaaaacattc ttgtaccagc accagttaat
     2041 tcaatatcaa tagtattacc attaccagaa attcctgtta ctaaaaaacc atcagtacca
     2101 tttattctta cagctccatc tgtagcatca atgttttttc cagcacctgc tccgccttgg
     2161 tcataagctt ggtctagatt gtttactgtt gccgtttgtg taattggcat ccattgtgta
     2221 ccattaaaaa agtaaaagcc aacaccattt ccaccaactg ttgttgcatt ggtattccag
     2281 atcaacaaac cggttgcggc ggtatttgtg ccatctaaag tggttaatgt tacattggtc
     2341 aaactaattc ttgggactaa aactccttta tctgttgctg aaatatccaa catagacgaa
     2401 gcatctggag tagttgtatt aattcctact tgagcaaatg aaaaagcggt gtagagtaaa
     2461 gcaaaataaa gtaattgttt tttcataata gtaaaatttt ttcccaacgg ttgtttagta
     2521 aaacgggttc tatcgtaatt tcgaaaagat ttggggtaaa acctttgata gcaatgtgtt
     2581 tgttttcgct tttcaaagtg tctgaaaaga cgatttttcg ttggattttg tccaaaattt
     2641 taatcaaata gtaaccgttt gtgtcggttt cgagtatgat tatcggttcg ttaatatttg
     2701 ccataggggt aactttacta taaaattaga gtaagttgta gtagcagtgg aattaaaatg
     2761 gtgaacagct gctttttttt agtgaacagt tgcttttagt aagtgaagaa tttaaagcaa
     2821 tcgaatgtgt tgcattaatt ggtcgcgata cgatttgcca acaggaattt tgtttttctc
     2881 aataaaaaga tagccttctt gaagcgaagt gattttggaa gtgtttacac aaaacgaacg
     2941 atgcactctt aaaaattgtg gttgtggaag tttatcttct acatttttta acgtataact
     3001 aatgagtagt ttttgagttg cagtaataac gtagcagtag ttatcaaaag cttctatgta
     3061 tagtaaatcg tctaaaacga tacgaataag ttcacctttg tttttgataa aaaaggaatc
     3121 agattcataa gcaacactaa ctgattgttt gtgaatttga tgtttgtgta atgccaattc
     3181 gatattcgtt tttagtgttt tttcgttaaa tggttttacg atataaccaa aaggttcggt
     3241 ttcttttacc ttttctaaag tttttgaatc ggataatgaa gtgagaaata taaatggaat
     3301 gtcgaatttc ttttttagaa aattcacaaa atcaattcca gttaaatcgc catctaaagt
     3361 aatatctact aaaaccaaat cgggttgcat ttgttctaca tcaaaaatcg cttctttaac
     3421 attgtctgcc attccacaaa tgatgtagcc ttctttttct agacatgttt taatagtttc
     3481 ggcaataagc ggttcgtctt ctagaatgta tattttagtt ggcatatcta attattgaag
     3541 ttcttcaaat ttagtgataa agagtgttat tgtagttcca tttttgtttt cgaaagtaac
     3601 tgttgctttt agttttttgg ctaatgaatt taccagtttt agtccaaaag aatttttgtt
     3661 ttcttgattt tttatacctg ttccgttatc ggcaacttgc aactccaatc cgttattttt
     3721 tttcatgaaa gaaaccatca actcaggatt cggatgtttt tctggaaagg catatttaat
     3781 tacgttggta atcaattcat ttacgattaa acccattggc gtaatggtgt cgatactaaa
     3841 aacatagctt tcacaatttt tggttacaag taaattttcg atagaaaaag attgattttg
     3901 aatgatatca tcaactaaat caacaagata ttcttcagaa tcaattccaa ttaattggtc
     3961 tttgctgtac agtttctgat gaattagaat cattgatttc actctgtttt gggcttcttt
     4021 tacagccatt gcggcttctt tatccacaat attttcggat tgtaagtata acaaactcga
     4081 gacaatttga aagctgtttt ttacgcgatg atgggtttct tttaaaagca catttttttc
     4141 atcgactaaa agttggagtt ggtttttttg tgtgttgagt tttttctttt gtttgatatt
     4201 taaaaaaact agataactca gaattaggat taagagtaat gatatgccga tataataata
     4261 aagcttttct ttttgctcta tttgcttttt aatctcttct tccttttttt gcgtttggta
     4321 tttggcttcc atttcataaa caacatcatc acgttcttga gcttttaagt cgtaaaacaa
     4381 atcaatatat tggtctttat catcagccgc tttttggtat tgacccaaat tgtaatgagt
     4441 tatactaatc gatgcgagaa ttatggtttt gtctcttaat gaagttgagt tttcaaataa
     4501 ttgttttgag ttttttaaaa tggtgtcaat gccaacatag cttttcaagc catttttagc
     4561 atctaataat ctcgaataat ctatggcatc gccacctttt attgggttgt attgtattgc
     4621 tttgacagca taattttcgg cttcgtcaaa tttttcttct ctaattttca tattacataa
     4681 attagaaaaa ataatgtttt ttatagtgtt tgaactagga taactcaagc ttttttggaa
     4741 atacttttca gcttctacat aattcccttc tcgatagttt atagtgccta tactcgacaa
     4801 actcagtact atagccgcag tgtctttttc tttttgagca taatcatatg atatattata
     4861 atactgttta gcttttttat aattatccga catatgaaaa actttcccta aagaatgata
     4921 tatatttccg agataattta atttgtattt ttcgcatttt tcaatgcatt ttaaaaaaat
     4981 cacttgtgct ccattatagt tgttttttac atacaactca taagagcctt ttgcttcttg
     5041 cactttcact tcgccaagtt tatagttcat ttttttagcg agtaaattgg ctttgtctat
     5101 gtatatataa atgctgtcat tttctgtatc aataagacta tcaattttat ccaaataagt
     5161 tttaaattct ggatagcttg ggtcttttga aaaatcgggt tgcttttttt gtgaaaagat
     5221 accgatgcca atgattaaaa agaatagtga aaatattttt ttttgcttta tcattgaaat
     5281 aaaaatgaat gttaagttta cattttataa aaatactaaa atactcataa tgttaaaaaa
     5341 aagagataat gtttagttta ttttcgtttt ttataactaa taacagttaa tttaattttt
     5401 tctgagtaac atttcactaa atttattcga aaataaaagt gaaattatga gtacagattc
     5461 gaaaaaaata gccaattcag aaaaaagcaa agtagcaaaa cttcaagata ttgttgctaa
     5521 aacaattgaa caagaaaaat taattactga aaatttatta catccgcctg aagaggtatt
     5581 gacaaaaggt caaaaaatat cagataaagt ggcgagtttt ggagggagtt gggcttttat
     5641 ttcctctttt tttatcattt tggttgcttg gattatcttt aataccatgt cgtctaaaaa
     5701 cctagaattt gatccatatc catttatatt gatgaattta attttgtctt gcattgctgc
     5761 acttcaggcg ccaataatta tgatgagtca aaaccgtcaa gaagaaaaag atagaaaaag
     5821 aagtgaaaat gattacttgg taaatttaaa gtcagaatta gaaatcaaag cattgcacga
     5881 aaaaatggat ttgttactcg aagaacaaat aaaaatattg tttgaaacac aagccaaaca
     5941 attaaagatt ttagaatcat tgcaaaaaga aaataaaaaa tgaatgtttt aattgttgaa
     6001 gatttcaagg aactggcaga cgaattagcg gagtttttat gtaaaaatgg ttatttatgt
     6061 aaaatagttt ctacttgcgc tgctgcatcc gaagaagttg aggttaacga atacgatgcc
     6121 atgcttttag atttgggttt gccagatgga acagggtttg agattttaaa aacagttaga
     6181 atattaaaat taaaaatggc agtaattatc atcacagcac gtggtgaact aaacgataaa
     6241 attgaaggat tagatcttgg tgccgatgat tatttaacaa aaccttttgc tttgaccgaa
     6301 ctcggagcaa ggttgtttgc ggtaattaga agaattcatg gctttgcaaa aaatgaatta
     6361 gaaattcatg gatttaaaat agcattaagc gattataaag tttcgtttaa tgaaaataca
     6421 atcgccttaa ccaaaaaaga gttcaatatc tttcaatatt tggtgctcaa taaaaataga
     6481 gtcatcactc gtttacaact aacagaacat gtttggggcg atgttttaga agttaattct
     6541 gactccaatt ttattgatgt tcatgttcga aatcttagaa aaaaattaga aaagtacaaa
     6601 acactttctt ggtttgaaac tgttagaaac gtaggttata gaataaacga ataaatgaaa
     6661 ataaaacatc aattagctgt tttcaatgtt ttaattcgat taattctcat atttgttttg
     6721 tggttttttt tgcccaaaat aatcgaaaaa gtagtctata atcacattga taaatcttta
     6781 tttgaaaaag aaaaaacatt tataaataga ctaaacaaac aagagataaa tgactttatt
     6841 agacgaaatg acgcttccga aacttacgct agcttttcga ctttgcatag tgagtttatt
     6901 cagctttatc aatcgaataa aaaattagat aatcaacaaa attacatcat aaatgaggct
     6961 agaataatag aaagcgaaga aagtaattac cgggttttat actatcattt caattatgcc
     7021 aatgccaatt atattttaga aattgggaat aatttaagcg aattagaaga catcattaga
     7081 gcacttcggt tttcggtttt aatgttgctt ttaattgtag ttagcgttac tttttttgcc
     7141 gatattttat tggtggaata cttgtttaag ccttttaata aaattattca aaccaaaata
     7201 aaatttgcca acgaaccaga taatttcaat ttcgaaaaaa tcaaatctca ctcttccgat
     7261 tttgatattt tggatacggg tttaaaccaa atgatgatac gtattaacga attgtttcaa
     7321 aaagaaagac aatttattag caatgtttcc catgaattat taacacccat ttcagtttta
     7381 aaaaatagat ttgaaaactt acttcaaaac gacacaataa atgaagaagt tgaagataaa
     7441 attgtcagct cattgcggaa tttagacatg atgaaaaaaa taattcataa cttattattg
     7501 atttcaagaa tcgaaaataa tcaataccaa cttgaagatt atgtttctat aaaacaatta
     7561 attaatgaaa tcatcaaaga ctttgaagac cgtatagaag aaaaaaatat tgaaatattt
     7621 accaattata atgaagacat tgtaattaaa ggaaataaaa tactattaca tattttatta
     7681 tttaatttga ttttaaatgc actaaaatac aattttgtaa atggtaaaat tgaaattgta
     7741 tcctatgaag aaaacaatca attttatctt tctatttcaa atactggaca tggtattgcg
     7801 aaagatcagt tggataccat ttttaatcgt ttttctagaa actcaaacga ccaagaaggt
     7861 cagggtattg gtttagcaat ggctaaaagc attgctaaag ttcatgctat tgaaatacaa
     7921 gtagcttcgt caattgatga taaaactaca tttacgctac ttttcccaaa gcaataaaaa
     7981 atagtaattt acaaatcttc atttaatcat catttgaaca atttactttt ggatataatt
     8041 ccgtgataaa tagtataatg gttttgttag atttgggaaa atcaagaaaa actggtgtaa
     8101 acgcatcagt tttttatttc tacttttttt atctaaaaaa ataaatcttc attttgtctt
     8161 catttaagcg tcatatcttt gacctgtaaa taatacaaaa acaaaaacaa ataattttaa
     8221 aaattagaaa tcatgaaaaa agtagtaatg ttagtagcaa ttctttttgg aacatcagta
     8281 atggtaaatg ccactactcc aaaaaaacaa gaagtaaaac aagaagccaa aaaagaagta
     8341 aaacatgcaa aaaaagcagt tgaagccaaa aaagtagctg ttaaaacaga agctaaaact
     8401 gaaatggcaa agccaacaat ggaagctaaa gaaaaaaaat aatcgattat tttttgatta
     8461 cattgatttt tggtgaagct gacaaaggga tttgtcagct ttttttatgc cagaatacgt
     8521 taaaaaataa ctaaaggtta ctttaatagt tgctatttct ttgttacttt gttaaaaatt
     8581 ataaaaacat gtttcgttgg ctttttgtat tcttattggt cgccattgtt gaaatttatg
     8641 cttttcaggc atttagaaca ctaacaaaag ttcgatcgta tcagtttgtt tatattatcc
     8701 taaaccttgt tgtcatcatc tatattgtaa atggattttt tcattttgat agaagtatcg
     8761 gacaaaccaa acaatcatta tttacaattg gtttactgtt attgacgtta gtgccaaaat
     8821 tgctcatcac ttttgtaatg attgtagaag atatatatag ggttttttcg ggaacaatta
     8881 cacgatttct tggagataac gattatggaa gttttttgcc cgaaagaaga aaattcgtta
     8941 gccaaatagc tgttggatta gctgccatac cgtttgtttc ctttttatac ggaatgacca
     9001 ttggaaaata taactttaaa atcatcaaac agacgctcac attcccagat ttgcctgata
     9061 attttgatgg atttgtttta gctcaaattt cagacataca tagcggaagt tttgataacc
     9121 ctgaaaaaat acaatacgct ataaatttaa tcaataaaca agaatttgat gtattacttt
     9181 ttacgggaga tattgtcaat actgatgcaa cagaaatgca tccttgggta gaaatgttca
     9241 aagggttgaa aaatccaact tatggaaaat attccatttt gggcaaccat gattatggaa
     9301 cttataaaaa ttggccatct gataaagcat tggaagataa ttttgaggca gtaaaagatt
     9361 tgcatcgtca aatagatttc agattaatgt tggatgaaca cgtgaaaata aagaaagaca
     9421 atcaagaaat tgcacttgtt ggagttgaaa attggggaaa aaactttgga gaaaaaggcg
     9481 acatcaacaa agcatctgaa aatttaacca aagaagattt taaaattttg ttgagtcatg
     9541 atcctactca ttgggaatat gtggttaaaa atcacgaaaa acattttcaa ttaacgcttg
     9601 caggacatac acatggtttt caatttggga tagaaattcc tggttggtta aaatggagtc
     9661 cagtacagta tgtttacaaa caatgggctg gattatacga aaatttagga agatatatat
     9721 atgtaaatag aggttttgga tttcatgcct atccaggaag agtgggtatt tggccagaaa
     9781 ttaccataat tgaactaaaa aaagacaaaa atgtagcata attagttaaa aatgctacat
     9841 ttgtgttata aaaattcatt tctttgtaaa aagaaattta atattttggt tttatgtcaa
     9901 aatttggaga attaattaac gctcaagtac cggtgttaat cgacttttat accgaatgga
     9961 atgattcatc ggtttctatg catcctgtaa ttagagatgt agcagctgca cttggcgaca
    10021 aagcaaaagt gattaaaatt gacgttgata aaaatcaaga attagccgaa gcacttcgta
    10081 taaaaggact tcctactttg atgatttata aagaaggtca aatggtttgg cgtcaatcag
    10141 gtgaattgga tgccaatact ttgataaata ttgttcaaga ccaactttaa attatttttt
    10201 cagaagaaaa acctttttct tttagaaact ccaaggtctt tggcaaaacg tattctaagt
    10261 ttttccaaga tttttgactg tcatgaaaaa cgataatgct accgttttca atatttttaa
    10321 gcacattttc taaacatttt tcttttgtaa tagtttggtc aaaatcataa gagattatat
    10381 cccacataac tattttgtat cccatttttc gcaatttttt agattgtgat ggttttattt
    10441 ttccatacgg cggacgaaat agtctgtagg ttttagtctg taaatcttcc tttttccatc
    10501 ttccatcttc caactctttt tggcataatt cagtattttc tacatacttc tcagtttccg
    10561 ttttccaacc attgagatga ttaaaagtat ggtttccaac gctgtgattg tgttttagga
    10621 tttgagagac tatttcggga aatttctgaa tattatctcc aatacaaaag aaacttgctt
    10681 tagcattgaa ggattttaat tgagccaaaa cccaattcgt aatttctggc gttggtccat
    10741 catcaaaagt tagaaatact tttttttcag ttgtttttat tttccaaata tggttaggaa
    10801 acaaccattt catccatatt cttgattttg cccaataact catgtggtaa agataagaat
    10861 aaaaaaaatg tgcaaaaatg aggtttcaaa tttgcacatt tattgaatta ataatcagta
    10921 ttattccatt tctcttccaa aacgagtaaa tcgtttgttg aaagcattaa atgtttcttt
    10981 gtttttgttg tagaatgcat catcgccgtt ttctttcata gtaatcagaa gacttctata
    11041 acgttcaatg tcagtaatga tgtcagtagc aattccgttt tgaatggaag gttgaatccc
    11101 tgaaaaatag gttaaacttt gtttgtactt agtcattaat ttttctagta attcacgagc
    11161 tttttctttt tctcctaatt tgtaatattc gccagcaaaa ggttctacca tggtgtaata
    11221 accataataa tcaataggca ttttggtgat agcttcatca attatttttt tggctttttc
    11281 attttttcct tctttgataa gctgctctac taatcttgac atattggttc ggtatgaaat
    11341 actgtttttt ctggtttcag tatcatgata aatagttgtc ttgtcaccat tgccccattc
    11401 ccacttagta accaagtcat acattttatc actgtcaatt ttccccattt cgatagaata
    11461 ttctctatca attttagttt taattggtac taatttatat accaatccat ccagttgaag
    11521 ataatctttc atccacaaat aatcatcatc gccaaaactt ccacctgtaa aataaacagg
    11581 tcttttccaa ttgttgttgg caataacatc aagcatcatg attctgtttt tatataaggc
    11641 tctgtcttta atgtccaaat cgatataagg cactatggaa tcatagtctt taggattaac
    11701 gactttgttt ttaataatgt tttctttgtc gatagtgatt ctaaacttat tggttgggta
    11761 gaaatgaact ttttgtccgt taggcaaatc aattgttgat tcgggaagtt ttataaagtt
    11821 caacatatct ttcaattccc aacgagcctc tgtttttaca attcgaactg cataatctaa
    11881 tttatcacca acatattcat cgtgtgtgaa tgaaattggc aaaccgtcag aagtataagt
    11941 tttgcgtttc atttgatcaa tgtaccaatc ggtcatgaat aattgtgtgt ttacaatttt
    12001 tatatcagga cgcacgtttt caatttcttg tgcataccaa agaggaaaag tgtcattatc
    12061 gccaatagta aataaaatag cattttggtc acaagattca agatagtttt ttgccattgc
    12121 gatagctgta tatttgccgc ttctatcatg atcatcccag ttttgtgaag ctattaatat
    12181 tggagcagca agtaaacata tacccacaat tactggtcca attaacttag atttcaaata
    12241 ttgtgatgtc ttttcgtata tagcatagac accaaagcca atccaaattg caaaaacata
    12301 gaatgatcca acaagtgcat aatctctttc tcttggttca aatggtcgtt cgtttaggta
    12361 tatttttaat gctaaacctg taaataggaa taaagcgagc agtacataga aacttttcaa
    12421 atctttattt gcatggtaga gtaatccaat aatccctagt aaaaacggaa ggaaataata
    12481 aaagtttcgt cctttgttgt ttttaatgtc atcggttaaa ttggaatcaa taccttttcc
    12541 aagatgaagt tcgtcaataa ctttaatgcc actcatccag ttaccatcta aattgtcata
    12601 ttttccttga atatcatttt gtcttcccac aaaattccaa agtaaatacc ttccatacat
    12661 gtaaccaaac tgatattcaa acatgaacca aaggttgtca aaggtggaag gtttttcaac
    12721 gattaagtaa tcgccatatt tttttaagaa gtttacatag tcttcattgt caactttttt
    12781 gtgagcatac gcctctttaa attctctgat tgaacttcgc aattcatcct ttaattgctc
    12841 cgtagcttgt gatatttgat cgctacttaa actgtctaaa ttcacaccat attgagctaa
    12901 atcttcttca aacggataat cataatttaa atcaaatttt ggaggacttg tgaatatcat
    12961 gtagttttca atatgatctg tgctccaaag tcttggtaaa atggctttgt gttgattgtt
    13021 tgtattttga tcagcatttt tccaattatt tacaattaca tattttccgg ttttgtaatc
    13081 tctttcgtaa tttggtgctt tgtctagata tggctcatct ggatctaatc ctgcaaaagt
    13141 atcagtgtat tgaggtccat aaaataaagg attaactcca tattgttcac ggttataata
    13201 cgctaaaact tcagctgcat ccgatggttt gttttcgtta attggagtat cggcatttgc
    13261 gcgaataggc aacatcatcc aagttgaaaa gcccaacaag acaaagagaa tacaaagaat
    13321 tatggtatta taaaacggat gtcctttttc gcgtgtatat tttaatccaa aataaaagaa
    13381 tcctaagatt aataaaacag cgaaaatagt tccagagttg aaaggcagtc cgaaattatt
    13441 gaccataaaa atctcagttt ttccaaaaaa tgctaatgta taaggtaaga gcaatttgaa
    13501 aataaatagc aaaatagcta cgataataat atttgcaagt acaaagtttt tgaccgtgac
    13561 ctttttgtaa tttttgaaaa aatataaaaa accaatagat ggaatcgtta ataatgccat
    13621 aaagtgaacg ccaaatgaaa gtcctattac taatgaaatc aataacaacc aacgattacc
    13681 ttttggggta ttcatgtctt cttcccagcg caatcctaac cagaacagca aagctataaa
    13741 taaagatgcc atggcatata cctctgcttc tacggcatta taccaaaaac tatctgaaaa
    13801 agtataagcc aatgcgccaa caaatgaact tccaagaatt acgattgatt ttgatttatc
    13861 tacttcgcta aattgagaaa ccaatttcct taatattatg gaagatgacc aaaacataaa
    13921 taatatggta aaagcacttg aaaatacaga aagcatgttt accattaagg caatagtttc
    13981 cttactcgta gcaaacattg caaagaatgc gcctagaatt tgaaaaagtg gtgctccagg
    14041 cggatgccca acttctaatt tggcagaagt agaaatgtat tcgccacaat cccaaaaact
    14101 catggttggt tcaacagtaa gcgtgaaagt aataagtgca attgcaaatg ttgaccatcc
    14161 taaaatggta ttccatttat taaagttaaa atgttgcata aatcaattta tatgtgttgc
    14221 ttttaaaagg tggtacaaag aaactatttt ttacgttaaa acttaaagta ttaacaaatt
    14281 tttctatatt attacaacgg attaattgac agattattgc attcaaatgt cttcaataat
    14341 tttataataa cctgtttttt tattaaattt acttgtataa aacaaaattt gttataaatt
    14401 tgcactcgca ttaagcaaat tggtctatgg tgtaatggta acacaactgt ttttggtgca
    14461 gtctttccag gttcgagtcc tggtagacca acaaaaaaat cctgcaattc gcaggatttt
    14521 tttgttttaa tatgactttg ttatctatat cttaaaagtt acaactggtc taacggcatg
    14581 atttactaaa tcttcactga tgcttccgtt aaagaaatgg gctaaacctt ttcttccatg
    14641 tgtactcatc cctattaaat cggcattgat gtgatttgca aagtttaaaa tcccgttttc
    14701 aacattagta tcattgtaaa tatgtttaga aaatttacgg atgctaaatt tagcaatgaa
    14761 ttcatccata atttgatgtg aaacatgcgt tggtttaaaa ttgctgggtg tattaatcat
    14821 aactaaatgc aattctgcat caaattcatt ggcaaattgg actacttttt caaaaggctt
    14881 tctaatttct tctgtaaaat cggacgcaaa aacaaactta tcaatattaa agtttgcgct
    14941 gtctttcttt atgattaaaa caggaatttc agaatttcta actacttttt cagcgtttga
    15001 tccaataaac atttctttaa atccacttgc accatgagaa cccataacaa ctaggtcaac
    15061 gttgttcttt ttgctaatat tcattattcc gtcaaaagcc aattcaaact gaattacttc
    15121 tgaaacattt atcccgtcta aacaaggatc gtccatcagt tcttcaagtt tattaattgc
    15181 tgcgttttta aacaacatta tttctggaag ttcatgaccg ttaccgatag catcgccagc
    15241 ttgactcggc aattctaaca tgtgtaacag aataacttct ccattatttt tttttgcaat
    15301 ttcagcagca acttttaagg cataatgtgc atgatcagaa aagtcagttg ggactaatat
    15361 ttttttcata aaaattgaaa ttttggtttt taaaattaac actttaaagg taataatatt
    15421 ttttgaatca acaatgtttt ttcatttata aaaaaataac tatatttgca ccgttattta
    15481 caattttgat tttaaatgat gagggaagca atgcttccct ctttttataa aaatgatgac
    15541 atttaaagaa aaagttagcg aacagattga aaatgcccta aaggtaaatc cttcattgtt
    15601 tttggttgat ttgaagattt cagaggcatt taaaatttct atagcaatag atggtgataa
    15661 tggtgtaaat cttcaagatt gcattgatgt tagtcgtttt attgaaaaca acttagatag
    15721 ggaagagcaa gatttttcac tagaagttgc ctcagcaggt gtttcaactc ctttaaaaag
    15781 ttttagacaa tacaaaaaaa atattggtag aacattaaag gttaaaacac tagctaatga
    15841 aacgttggaa gcaaaattag aagatgtaaa tgatgatttc ataacgttgt cttggtcttc
    15901 aagagaacca aaaaaaatag gtaaaggaaa agaaactgtt caaaacaaca gagaaatttc
    15961 ttattcagat ataaaagaag caattgttac aataatattt taattaaaaa ttaggcatgg
    16021 agaatattgc attaatcgat tcgttttcag agtttaaaga cgacaaactg attgatagag
    16081 tgacgctgat ggcgattttg gaagatgttt ttagaaacgc tttaaaaaag aaatacggtt
    16141 cagatgataa ttttgacatc attatcaatc cagataaagg tgatatggaa atttggagaa
    16201 gaagaatcgt tgtggcggat gatgatttgg atttagaaaa tgaagaaatt acattgactg
    16261 aagcaagaaa aatcgaaccc gattttgaaa ttggtgaaga agtatcagaa gaagtaaaac
    16321 ttattgattt aggaagaaga tctattttgg cattgcgtca aaatttaatt tccaaaatcc
    16381 acgagcatga tagcactaat ctttacaaac aatttaaaga tttaatagga gaaatatata
    16441 ctgccgaagt gcaccatgtt cgtccaagag ttgtaatttt ggtagatgac gaaggaaatg
    16501 aaattgtttt gccaaaagaa aaacaaattc cgtctgattt tttccgcaaa ggtgacaacg
    16561 ttaggggaat tattgaaaat gttgaactaa aaggcaataa gcctcaaatc atcatgtcaa
    16621 gaacggcaga taaatttttg gaaaaattat ttgaacaaga aattccagaa gtgtttgatg
    16681 gtttgataaa cattaaaaat gtagtaagaa ttcctggaga aaaagcaaaa gttgctgtag
    16741 attcgtatga tgacagaatc gatccagttg gtgcttgtgt tgggatgaaa ggttctcgta
    16801 ttcatggaat agttcgtgaa ttaggaaacg aaaatatcga cgtaattaat tatacagcaa
    16861 atattcaatt gtatattact agagcattaa gtccagcaaa agtatcttct gtgaaaatta
    16921 atgaagaaac taaaagagca gaagtgttct taaagttaga tgaagtttct aaagcaattg
    16981 gtagaggtgg tcataatatt cgtttggctg gacagctaac tggttatgaa cttgacgtta
    17041 tccgagaagg aagcgaaatg gagaatgatg atgttgagtt atcagaattc agcgatgaaa
    17101 ttgatggatg gataatagac gagtttgcaa aaattggttt agatactgcc aaaagtattc
    17161 ttagtcaaaa tgtagatgac ttggttcgca gaacggattt agaagaagaa actattttag
    17221 aagtaataaa aatattgaaa gaagagtttg aagactaaac tttcagaaaa agaaaacaga
    17281 taacattaat aagtatatat gtctgaagga aatattagga ttaataaagt tttaagggaa
    17341 ttaaacattt ctttggaaag agctgtggat tatctaaaag ataaaggagt tgtcattgaa
    17401 gcaagtccaa acacaaagat ttccgatgat gtttacaatg ttctttgcgg agaatttgct
    17461 ggagacaaag ggaagaaaga agcttccatg ggtatcagtg aagaaattcg taaagaaaaa
    17521 gaagctattc gccttgaaag agaaaaagaa attgaagaca aaagaaaact tgatgaagaa
    17581 cgtcaacaac aagaattaat caaagcgaaa gcagttgtta gtgctccaaa acaggtaggt
    17641 aaaattgatt tagaaaaaaa acctgcttca acttcagtgc ctaaaatttc tgcagacaaa
    17701 aaagaagaaa ctcctaagtt tgaaaaagca acaccagtta aaaaatcaac tgaagtagaa
    17761 ctcgaaaaac caaaaagtaa agtagctaaa aacgagactg ctgaagaaaa cgttgaaact
    17821 cctgcagaag aagctatcga gactaagtat caaaaattat ctggcaccaa gcttacgggt
    17881 caggttatag acttatcaca gtttaataag ccaaaaaaga aaaaagaaga acctaaaaaa
    17941 gatggcaagc caaatgttcc tggaaccaat tcgggcaata acgccaataa gaataagaga
    18001 aaaagaatac caaccaaacc aggtgaagga agagcaataa cgccaggtaa tactaattca
    18061 aaatttggag gtaaaccagg cggaggattt caaaaaggta cacgacctgc tattgttcaa
    18121 aaagtggaac ctacagaaga agaagttaaa aatcaaatta aagaaacttt agagcgactt
    18181 caaggtaaaa aaggaggaaa atctaaagca gcaaaatata gaagagataa gcgtgataca
    18241 caccgtcaaa gaacggaaga tgaattgcaa gcgcaagaag aaggaagcaa aactttaaaa
    18301 gtgaccgagt ttgttacagt cggtgaaatt gcaaccatga tggatgtgcc aattactaaa
    18361 gttataggaa cttgtatgac tttaggaata atggttacca tgaatcaacg tcttgatgca
    18421 gaaacacttt caatagttgc agatgaattt ggatatgaag ttgaatttat cactaccgat
    18481 atagaggaat ctattattca agttgaagac aaaccagaag atttagtttc tcgcgcgcca
    18541 attgttaccg taatgggaca cgtggatcat ggtaaaacat cattgttaga ttacattcgt
    18601 aaagaaaatg tgattgctgg tgagtcgggg ggaattacac agcatattgg agcttatgcc
    18661 gtaactttag ataacggtca aaaaataaca ttccttgata ctcctggtca cgaagcgttt
    18721 actgcaatgc gtgctcgtgg tgctcaagtt accgatattg caattattgt tgctgctgcc
    18781 gatgatgata ttatgcctca aaccaaagaa gctatcagtc acgctcaagc agcaaatgta
    18841 ccaatgattt ttgctatcaa taaagtagat aaacccgccg ctaatccaga aaagattaaa
    18901 gaacaattgg caggtatgaa cttacttgta gaagattggg gcggaaaata ccaatcacat
    18961 gatatttctg caaaaatcgg aacaggtgtt aaagatttat tagaaaaagt attgcttgaa
    19021 gctgaaattt tagatttaaa agcaaatcca aaaaaaccag caattggaac cgtaatagaa
    19081 gcacaacttg ataaaggtcg tggatatgtt acaacaattt tagtacaggc aggaacttta
    19141 aaagtcggcg attatgtttt ggcaggaaaa catcacggta aagtaaaagc aatgtatgat
    19201 gaacgtggtc attctatttc aatagctagt ccatcaacac cggtttctgt attagggtta
    19261 gacggtgcag caacagcagg agataaattt actgttttcg aagacgaaaa agaagcaaaa
    19321 caaattgctg caaaacgtac ccaattaatg cgtgagcaat cagctagaac aaccaaacat
    19381 actacacttg ctgagcttgg tcgtagaatt gctttagggc aatttaaaga attaaatata
    19441 attattaaag gagatgtgga tggttcagtt gaagcattgg cagattcatt ctcaaaatta
    19501 tctaccgaag aaattgaaat caaaatcatt cataaaggag ttggagcaat taccgagtct
    19561 gacgtaatgt tagcttctgc atccgatgca attattattg gatttaatgt tcgaccagca
    19621 ggtaatgcaa aacagattgc tgaaaaagaa gaaatcgata tccgtaacta ttctattatt
    19681 tatgatgcta ttgatgattt gaaagacgcc atggaaggaa tgctttcgcc agagttgaaa
    19741 gaagaaatta caggaacagc agaaatcaga gaattgttca aagtgtctaa agtaggtacc
    19801 attgcaggtt gtatggtaac cgatggaaaa atatttagaa gttcacgcat tagaattatc
    19861 cgtgatggtg ttgtaattca tacaggtgaa ttaacagctt taaaacgttt caaagacgat
    19921 gtgaaagaag tttctaaagg ttatgattgt ggtatgcaaa tcaaaaacta caataatatt
    19981 gaacaattag atattattga agcctatcaa gaagttgaag tgaagaaaaa attgaaataa
    20041 catcaatttt acacgaaaca taaatattaa cctcaatatt caaaattgag gtttttttat
    20101 tttttttctc aactttaatt tgaagcttcc ttgtcttagt aaaaataacc ctttcagagt
    20161 tctaaactct gaaagggtta ttgattttgg cctaaagatt tagggtgtta tttattaggt
    20221 ttcactaaaa atgattttgg aaatttcttt ttaatttcaa taagatttcg ttccgcttca
    20281 attcttgttt taaaatttcc aacccatacc ttatataaag gtgtgctaaa tacaatggta
    20341 caatctaatg ttccaaattc ttttttgaaa gccatcaatt gtttttttga actttcgtta
    20401 tcaccattgt aaatttggat tttataacgg tcggttatag taatcgaact gttgattttt
    20461 cttttttcat ttagcaattg ttcaaatttt gggtcttgcg aaatgctgac aattccttct
    20521 tgagagtagg attttaaact aattttagca agtaatgcta agactaaaaa gggtttaaag
    20581 aatgttaaaa att
//
LOCUS       DAOOBH010000028        20499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_3445_length_20499_cov_5.627568, whole genome shotgun sequence.
ACCESSION   DAOOBH010000028 DAOOBH010000000
VERSION     DAOOBH010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 20499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20499
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3445_length_20499_cov_5.627568"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="PK218_04830"
     CDS             complement(<1..1498)
                     /locus_tag="PK218_04830"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HPW97864.1"
                     /translation="MKKKLLYLLIIVFVGFSVHGQFSKTHYIPPLSNSSSMIIGNKFI
                     YISTPSINPINVQLKEIGGNTINASVSRDAPFVYDTNSGTPDQLFVNQNQVNEVLSNK
                     GYIIEAEDVVYVSVRVDTSDGFQAGALVSKGLASLGTEFRIGGFLNTLVQSYSNIHHT
                     FITVLATENNTEVSFSNIKPNATLLNSTTGSNPFTVVLNSGESYAVAVQGPNTNNRDA
                     LIGALVNSDKPIVVNCGSIGGTNGELSNLDLGFDQIVGAQRVGNEYIFLRNTGQDNVE
                     RVLLIANEDNTGIYMNGSTTPSYILNAGDYGTYIGADFNPEGVLYIRSADATDPLIKK
                     NVFAYQSIGDNVFTGQANQEMFFVPPLSCQTPKVINNIPLIEDLGGRTFTGRVTITTQ
                     VGATLNFIINGVNYTLAGLNGIGINVQGPIAVTGNPNYECYILIGLSGNVAAYSTGEL
                     YLAAYGSDGAATFGGYYSGFTFNPEISFNRLDITSANCIPNTILGVNTL"
     gene            complement(1583..2428)
                     /locus_tag="PK218_04835"
     CDS             complement(1583..2428)
                     /locus_tag="PK218_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW97865.1"
                     /translation="MRSHERLLSIELQKIWKNRASKVLTISYFVILSFIALIASIKFD
                     IGNFKIHFAEMGIFNFPFIWHFNTYIASTLKFFLAIIIVSMMANEYSYGTLKQNLIDG
                     MSKKEFILSKFLTVVFFAVVSTIFVFIMSLLLGYNFSSYNEIGIVFSDLEYLVAYFVK
                     LVGFFSFCLFLGVLIKRSAFAIGFLFIWFIGETIARLTLTFDIFPKSKIASQITQFFP
                     LESMSNLIVEPFSRLSVIKNIQETIGENNSKDFGVHPIAILIVLAWTAIFIYSSYLLI
                     KKRDL"
     gene            complement(2421..3326)
                     /locus_tag="PK218_04840"
     CDS             complement(2421..3326)
                     /locus_tag="PK218_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW97866.1"
                     /translation="MKTAILSIKNLHKRYGKVHAVNNVSLDIHKGNVYGILGPNGSGK
                     STTLGIVLNVVNKTSGEYSWFGGTQETHQALKKVGAIIERPNFYPYMTAKENLELVCK
                     IKGISYSKIDEKLEIVGLTDRKDSKFKTYSLGMKQRLAIASALLNDPEILILDEPTNG
                     LDPQGIIQVRDIIRFIASQGTTILLASHLLDEVEKVCSHVLVLRFGEILYNGKVDEMT
                     SKSNYFELQSDNNNLIIDLLKNNFTVDTISEVDGKIEVYFNQELEASTLNRFLAEKGI
                     YLNHLVKRKLSLENQFLALTNQNNS"
     gene            complement(3375..3641)
                     /locus_tag="PK218_04845"
     CDS             complement(3375..3641)
                     /locus_tag="PK218_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97867.1"
                     /translation="MKTNYLADKIVSKNLICILFGHHYVEIKKVNNHFKEFECSHCKL
                     QATNDIKGKKTALTTELKDINKTLSYLHLKREFLSHFYFSRNKQ"
     gene            complement(3756..5510)
                     /locus_tag="PK218_04850"
     CDS             complement(3756..5510)
                     /locus_tag="PK218_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97868.1"
                     /translation="MDSTKVMYQKIEKYSNKSNFRKFLHKLIFSSTQRSKPSNNNLRD
                     KIDEKIGKSEGKIIRNIDIVSLDPFGYSDEDENREPHNSFEIFGNKIHIKSKKWTIRN
                     NLLFKKNQPLDSLLIKESERLLRRQRFVRSVLIKTIPIENNVDSIDVSVRVLDTWSLI
                     PNGAISGSRMNLELTERNFFGLGQEFENDFGKQFSDNKNSYSGKYSFNNIKNTFIDAS
                     FSYSSSFNNDVSKRVIVERTFFSPITKYAGGVSYETQKYTDSLPTILPEFETIIFENE
                     TKSFWLGNASKLFKGKSIDFRSTNFVTTIGYKKVNYIRNASIMIDPSQFLSGEELYLI
                     SFGITTRKFIQDKYLFNFDIIEDVPFGKVYSITAGFQNKNNTRRNYYGGRFSYGHYYP
                     FGYLSTNIELGSFFNDTKTQETTLKIEANYFTSLLSLGNWRIRQFIKPSLVVGINREN
                     SIKDRISLNSLNGFDGFTNPLFNGTKKLSVVFQTQTYAPGNWKGFHFSPFINTSFGLL
                     GDSHDSFLNNKLYSKFTLGVLINNDYLVFNRFQVSFSYYPSIPFEDSNVFKTNTLKND
                     DFNIPDYTIGQPTIVDFR"
     gene            complement(5617..7113)
                     /locus_tag="PK218_04855"
     CDS             complement(5617..7113)
                     /locus_tag="PK218_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HPW97869.1"
                     /translation="MKKIKFPSAQTILLLIAAMVTLLTWVVPAGKYNRLSYDKETKMF
                     NIKNGEASKTVAGTTKTLDSLGIKIPIEKFTSGDIWKPINIPNTYEEVDAKPQGFFDL
                     VKSPIKGIIAGADIIFLVLFIGGLIGLMNETGAFDAGISWLAIKLKGKEYILIILVTA
                     LISVGGTTFGLAEETIAFYPILIPVFLAAKYDAMVALASIYVGSCIGTMCSTTNPFSA
                     IIASNAAGINWTSGLNTRFVAYVLGTLICIIYIMRYAKKVKNDASQSIIYDQKEEIEN
                     LFKNNTESNLQLTTRLRVILFIFLLCFVVMIYGVAKLEWWFLEMTTVFFVGSILIGFV
                     ARIKEAKFIETFVKGAGELLSVAIIIGIARGITVIMDDGMISDTMLYYASHITEGMNK
                     GLFANVLFVIYGGLSFFIPSSSGMAVLTMPIMSPLADTVGIGRETVVDCYQYGMGLFS
                     FISPTGLILASLAIVKVGYDKWLKFVLPLVFILFLFSIVMLTISVYLT"
     gene            complement(7218..8276)
                     /locus_tag="PK218_04860"
     CDS             complement(7218..8276)
                     /locus_tag="PK218_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HPW97870.1"
                     /translation="MINLFNAHIESLSIHRVGNKSRNEAIFLSEQPYNLNDEIVPLLK
                     EYFFKPFREKEENYFQFAHDVDLDYNEMYNLATEVFTNPSDVHSISKKITSHLFEQSN
                     HPHIKNGEVYVAYITNISIDNNVVDAIGVFKSEIKTDFMQFEEKGTTLEMILQQGISL
                     NKQDKGCLIFNYKKEEGYKILTVDSNRYDARYWLEHFLSVDAFQDENFFTKKYLKFCQ
                     GFAKDVVLPAEDKKEEVMFMNRSVNFFAKNDEFEESKFLNEVVNNPDLIPEFKNYKVD
                     KGEKYSIEDVTNFPIANAAVSDARKSIKNVINLDTNIQIKLDFINPESAEKFVEKGWD
                     EEKQMYYYLVYFNKEQKS"
     gene            complement(8320..8714)
                     /locus_tag="PK218_04865"
                     /pseudo
     CDS             complement(8320..8714)
                     /locus_tag="PK218_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494566.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(8768..9307)
                     /locus_tag="PK218_04870"
     CDS             complement(8768..9307)
                     /locus_tag="PK218_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97871.1"
                     /translation="MIYKFRAILDAEDDVFRDIAIHQEDTLEDLHNAIVNAFGFDGME
                     VASFYTCDDTWNQEDEIPMFDSGDVLGEQKTMADYTLNDVLNLEQTKIIYVYDFINMW
                     TFLVELAAVEEPVIGETYPALLFSHGELPAEAIEKQFEAENQDDYYSEFEDDIDEDDL
                     DAFGSDDSFEDYGFEENWN"
     gene            complement(9425..9814)
                     /locus_tag="PK218_04875"
     CDS             complement(9425..9814)
                     /locus_tag="PK218_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97872.1"
                     /translation="MKTIKSIVILLVLTSFSQMNAQKILDKWEDLNTVNQIYTKVAYV
                     ANEGNFEVIGQYTTLLKKYSEKLDTKSIPAELVTPAITEMVGKLKSEAAALDEVAQNK
                     GSKQDVYAKMKPFGETLNILINSIKTE"
     gene            complement(9920..10735)
                     /locus_tag="PK218_04880"
     CDS             complement(9920..10735)
                     /locus_tag="PK218_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPW97873.1"
                     /translation="MKKTICFLIVIMTLTSCANKQQNNDPIPKHDEFTIQSKKVGEKR
                     TINVWTPPMYAESTDSIPVLYMPDGGLKEDFPHIANTLAKLIKNKSIPPMMLVGIENT
                     ERRRDLTPFTMVEKDKEVAPIIGGADKFRDFITDELMPEINKRYRTTNQKGIIGESLA
                     GLFVMETFFKKTETFDFYIAFDPSIWWNNHELERDGAKYLQNFTTKNTKVWFAGSGAE
                     DISQFTTKLAKTFDSKAPSNITWTYSDEPNEKHSTIFRATKEKALVWTLGNKE"
     gene            complement(10817..11023)
                     /locus_tag="PK218_04885"
     CDS             complement(10817..11023)
                     /locus_tag="PK218_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97874.1"
                     /translation="MNAFWLVSIIIFITTTFLLSKVTLKKYQTEIGEKMWKSYGSRTY
                     YWKLLLLISFGITALIVFVLKMVV"
     gene            11234..11608
                     /locus_tag="PK218_04890"
     CDS             11234..11608
                     /locus_tag="PK218_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HPW97875.1"
                     /translation="MGTFVISKRVNGDYKFIFATRKGKTIFTSIGCKNKSDCEKIIAA
                     IQGNIEQFTLTRSKNAAGKYFFRLAKDGLVLATSRKFSTEFMFQKGIDNIKEHLPNSE
                     TLDFSENEFVFPDSEAVFVEEE"
     gene            complement(12202..12555)
                     /locus_tag="PK218_04895"
     CDS             complement(12202..12555)
                     /locus_tag="PK218_04895"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW97876.1"
                     /translation="MDTLLFDAFNNQNMAQFKPLFTEDLEWYQDNGGLIPYETVFENL
                     ENTFKKEDKLTRQLVKGSLEVHPIKDYGAIQIGVHQFRHIENGKEEIGTFKFAMLWKK
                     TNDKWQISRVISYDH"
     gene            complement(12808..13023)
                     /locus_tag="PK218_04900"
     CDS             complement(12808..13023)
                     /locus_tag="PK218_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97877.1"
                     /translation="MSPEEEKIIVNMLLLQDSMKHRKDAMSKIEEMANDYDQLIQFID
                     NKWDDLTENEKNQVLDRYKQEREKRKS"
     gene            complement(13811..15799)
                     /gene="uvrB"
                     /locus_tag="PK218_04905"
     CDS             complement(13811..15799)
                     /gene="uvrB"
                     /locus_tag="PK218_04905"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPW97878.1"
                     /translation="MKFQVVSDYKPTGDQPQAIEKLSNGILNGEKYQTLLGVTGSGKT
                     FTVANVIQNVEKPTLILAHNKTLAAQLYSEFKQFLPNNSVQYFVSYYDYYQPEAFIPV
                     TGTYIEKDLSINEELEKLRLSTTSALLSGRRDIVVISSVSCLYGIGNPVEFQKNVVSI
                     AKGEVISRTKLLHRLVQSLYSRTEAEFLPGTFRIKGDTVDIFPSYADDPFRVHFFGDE
                     IEEIEAFDHRSTKVIERYEKLNIYPANMFVTSPDVLQAAIWDIQQDLVKQVDYFKEIG
                     KHLEAKRLEERTNFDLEMIKELGYCSGIENYSRYLDRRLPGTRPFCLLDYFPDDFLMV
                     VDESHVTISQVHAMYGGDRSRKENLVEYGFRLPAAMDNRPLKFEEFESMQNQVLYVSA
                     TPADYELQKSDGVYVEQVIRPTGLLDPIIEVRPSLNQIDDLIEEIQLRCEADERVLVT
                     TLTKRMAEELTKYLTKVAIRCRYIHSDVDTLERVEIMQDLRKGLFDVLIGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSHRSLTQTVGRAARNVNGKAIMYADKITNSMQKTIDET
                     NYRREKQTAYNTLHKLEPKALNKSLGNALSGNSISTNYYHEKALDAAEPESLYLSKPE
                     IEKKIRELRKAMEKAAKDLDFIHAAKFRDQIEALQGKL"
     gene            complement(15803..16099)
                     /locus_tag="PK218_04910"
     CDS             complement(15803..16099)
                     /locus_tag="PK218_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPW97879.1"
                     /translation="MAERIIIWTTTAKIELKFILEYYNLRNKSKTYSQKLLRTIQSEI
                     KLLVQQPFIGKKTDTINVRGLLIENFIVFYEVNESHIIILSVWDTRQNPATLNL"
     gene            complement(16084..16314)
                     /locus_tag="PK218_04915"
     CDS             complement(16084..16314)
                     /locus_tag="PK218_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97880.1"
                     /translation="MNTIELKKILISKINEINDEAYLKAINTILDSKSENILMLSDNQ
                     KSSIQKSRKEYLEGNYQTNDTVNEEMEKWLKE"
     gene            complement(16352..17308)
                     /gene="rlmF"
                     /locus_tag="PK218_04920"
     CDS             complement(16352..17308)
                     /gene="rlmF"
                     /locus_tag="PK218_04920"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HPW97881.1"
                     /translation="MKKIQNTYSEKSNFHLRNPHRFRYDFDQLIIDSPKLKQFVLVNE
                     HDNQSIDFSNPEAVKALNNAILISEYNIQNWDIPMGYLCPPIPGRADYIHYIADLLAD
                     SNDGIIPKGEAVTGLDVGIGANCIYPIIGNAAYDWSFVGCDIDENALQNCKKIIANNP
                     KLIDAISLQLQTEPRFIFKNIILPEDKFAFTICNPPFHNSAEDAHKAALRKVNNLSKS
                     KTDKTILNFGGKQHELWCAGGEFAFITQMIYESVKYPLQVFWFTTLVSKKDNLKSIYR
                     LLNKVGAVEIKTIDMAQGQKISRMVAWTFLSEDQQKNWKFSE"
     gene            complement(17309..17971)
                     /locus_tag="PK218_04925"
     CDS             complement(17309..17971)
                     /locus_tag="PK218_04925"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPW97882.1"
                     /translation="MSSHHIVRDDQEPALIIANGASCSNELLGQLLEWSPYVIVLDSA
                     IERVLDLNIKVDVLLGDFDRNFDTEKIKERQYPIEIVHTPNQDKTDLEKAFDYLIEKG
                     HKAVNVIWATGKRADHTITNITNIIAYRNQLKIVLLDDHSKVFLLPKKFEKWYTANTI
                     ISLIPVGKVTGITTQNLFYPLNNEELTIGYRTGSSNHVSEDGIVKIEHQEGDLLLMEC
                     WD"
     gene            complement(17978..18796)
                     /locus_tag="PK218_04930"
     CDS             complement(17978..18796)
                     /locus_tag="PK218_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HPW97883.1"
                     /translation="MKTSKYILILVSFIFLSCEKVIDVGLDTAAPRLVIEASINWVYG
                     TVGNQQKIKLSTTTDYFSNTVPPVTDAIVFITNSSNQQFNFVQDTEPGIYNCADFNPV
                     LDETYTLTVMYKNEVYTSTEKLLNTPIVKRVEQKNDGGILGNATEVKFVFDDIPNETN
                     HYFLKINDPYKVIPEFGVLEDRFFQNNEMFGLYFSEDLKTGDTIKFTNNGITLNYYNY
                     MNILLSQAGGGNAGPFSTPTSTIRGNIVNQTNFDNFALGYFRLSKTEIKEYIIE"
     gene            complement(18800..>20499)
                     /locus_tag="PK218_04935"
     CDS             complement(18800..>20499)
                     /locus_tag="PK218_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW97884.1"
                     /translation="DIYQKEGNKDSFHMNGGIGLISSRLLAEGPIKKGKGSFVVAGRG
                     TYAHLFLKLANNSNSAYFYDLNTKLSYNLNQNNNIYLSGYFGRDVFSLNENFVNTYGN
                     AVVNFRWNHLYSDKLFSNLSLIYSDYYYGLTLEFIGFNWNSGIKNYNIKYDFKHYLNN
                     KLKLTYGFQSNYYDFNPGKIEPTGTGSDINPEQLDKKYALENAVYFDVEQDISKKITI
                     SYGFRLSNFLRLGQQTLNTYENNQAVIFNPNFQIYESAEPTGTVSYGKNQTIAHFENF
                     EPRLAISYAINDHQSVKASYNRMSQYLHLISNTQSPTPLDVWAPSDNFLKPQLLDQVA
                     LGYFQNFKDDDYSLEVETFYKKIKNRVDYIDGANLIANEAIERVILNGKARAYGLEVY
                     FRKNAGRFSGWLSYTLSRTEQQVEGRTSIETGINNGDWYKTGFDKTHNISLVGSYKLN
                     EKWRFGGTFTLQTGQPVTFPNGQYQYEGITVPSYSTRNENNLPTYHRIDVSATLTPRK
                     NKGRKWQSEWVFGIYNLYNRYNAASITFRQNDKTAQNEAVQLSIFGVVPSATYNIKF"
BASE COUNT         6321 a         3350 c         3434 g         7394 t
ORIGIN      
        1 ttaaggtatt tacacctaaa atagtatttg gaatgcaatt agccgaagta atgtccaatc
       61 gattaaaaga aatttcagga ttgaaggtaa acccagaata atagccgcca aatgttgcag
      121 cgccatcact tccataagca gcaagatata attctcctgt agaataggct gctacattac
      181 ccgataaacc aatcagaata tagcattcat aatttggatt tcctgtaact gcaatcggtc
      241 cttgtacatt tataccaatt ccatttaaac cagctaaagt gtaatttact ccattgataa
      301 tgaagttcaa agtagcgcct acttgcgttg tgatggtaac tcgacctgta aaagttcttc
      361 ctcctaaatc ttcaattaat ggaatgttgt taattacttt gggtgtttga cagcttaatg
      421 gtggtacaaa aaacatttct tgattggctt gtcccgtaaa aacattatca ccaatacttt
      481 gataagcaaa tacatttttc tttataagtg gatcagttgc atctgcagaa cgaatgtata
      541 aaacaccttc tggattaaaa tcggcaccaa tgtaggtacc ataatctcct gcatttaaaa
      601 tataacttgg agttgtactc ccgttcatat aaatgccagt gttgtcttca ttagcaatta
      661 ataaaacccg ttctacattg tcttgaccag tatttcttaa aaaaatatat tcattcccga
      721 cacgttgtgc ccctacaatt tggtcgaagc ctaaatccaa attgcttaat tcaccattag
      781 taccaccaat agaaccacag tttactacaa tgggtttgtc tgaattaacc aatgcaccaa
      841 ttaatgcatc tctattgttt gtgttgggac cttgaactgc tactgcatag ctttcaccac
      901 tattaagaac tactgtaaaa ggattgctgc cagtggtgct atttaataaa gtagcattgg
      961 gttttatatt gctaaaacta acttcggtat tattttcggt agctaaaaca gtgataaagg
     1021 tgtgatgaat attgctataa ctttgtacta aagtgtttaa gaatcctcca attctgaatt
     1081 cggttcctaa agaagccaat ccttttgaaa ctaatgcacc agcttgaaaa ccatctgaag
     1141 tgtccactct aacggataca tagactacat cttcagcttc aataatgtaa cctttgtttg
     1201 aaagcacttc gtttacttga ttttgattta caaataattg atcaggagtt cctgaattag
     1261 tgtcataaac aaaaggagca tccctagaaa cagaggcatt tatagtattt ccgccaattt
     1321 cttttaactg aacatttatt gggtttatgc ttggtgtaga aatgtaaata aatttatttc
     1381 caataatcat tgaacttgaa ttggataatg gcggaatata atgggtttta ctaaattgtc
     1441 catggactga gaatccaaca aatactatta ttagtaaata gagtaacttc tttttcattt
     1501 gctaaaatta acaaataaaa agaaaactca aatcagaatt taatctattt aagttttctt
     1561 taagaaatga ttgatgaagc tattataaat ctcgtttttt gattagtaaa tatgagctat
     1621 aaatgaatat agctgtccaa gctaaaacta tcaaaatggc aattggatgt acaccgaaat
     1681 ccttactatt attttcccca atggtttctt ggatgttttt gatgacagat aatctcgaaa
     1741 aaggttctac aattaaatta ctcattgatt ctaatgggaa aaattgagtg atttgtgaag
     1801 cgattttgct ttttggaaag atatcaaagg ttagtgttaa tctcgcaatt gtttctccaa
     1861 taaaccagat gaataaaaaa cctatggcaa atgcagatcg ttttattaaa actccaagaa
     1921 atagacagaa tgagaaaaaa ccaacaagtt taacaaaata ggctactaaa tattccaaat
     1981 cagaaaacac aatgcctatt tcattataag acgaaaaatt atagcccaaa agtaatgaca
     2041 ttatgaatac aaaaattgta gatacgactg caaagaaaac aacggttaag aactttgaaa
     2101 gaataaattc ttttttgctc attccgtcta ttaggttttg ttttaaagtg ccataactat
     2161 attcatttgc catcatagaa acgataatta tggctaaaaa gaatttgagt gttgaggcaa
     2221 tataggtgtt gaaatgccat ataaatggaa aattaaaaat ccccatttcg gcaaaatgta
     2281 ttttaaaatt tccaatgtca aatttgatgg aagcaattag tgcaataaaa gataatatta
     2341 caaaatagct aatagtcaat actttactgg ctctgttttt ccaaattttt tgaagctcta
     2401 tggataataa tctttcatgg ctacgaatta ttttgattgg ttaatgctaa aaattgattt
     2461 tccaagctta gttttctttt tactaaatga ttgagatata ttcctttttc agccaaaaat
     2521 cgatttaaag tcgaagcttc taattcttgg ttaaaataaa cttcaatttt tccatcaact
     2581 tcagaaatcg tatctactgt aaagttgttt tttaataaat caatgatgag gttattatta
     2641 tccgattgca actcaaaata gttgcttttt gaagtcattt catccacttt tccgttgtat
     2701 aaaatttcgc caaaacgcaa aactaaaaca tgactacata ctttttcaac ttcgtctagt
     2761 aagtgcgaag ctaataatat tgttgttcct tgtgaagcaa taaatcgaat aatatcacga
     2821 acttgaatga ttccttgtgg atctaaccca ttggttggct catcaagaat taatatttct
     2881 ggatcattta atagtgccga agcgattgcc aatcgttgtt tcatccctaa ggaataggtt
     2941 ttaaatttgc tgtcttttct atcggttagc ccaacaattt ctaatttttc atctattttt
     3001 gagtagctta ttccttttat tttacaaacc aattctaagt tttctttggc ggtcatatag
     3061 ggataaaaat taggtctttc gattatagcc cctacttttt ttagtgcttg atgcgtttct
     3121 tgtgttccgc caaaccatga atattcgcca gaggtcttgt tgactacatt caaaacgatg
     3181 ccgagtgttg tagattttcc agaaccattt ggaccaagta tgccatagac atttcctttg
     3241 tgaatatcta atgaaacatt gttaacagca tgtactttac cataccgttt gtgaagattt
     3301 ttgatagaaa gaattgcggt tttcaagtga ggttgattaa aggtttctct taggacgaat
     3361 gaaacctata aatgttactg cttatttctt gaaaaataaa agtgagatag aaattctctt
     3421 tttagatgca aataggaaag tgttttattg atgtctttta attcagtggt caatgctgtt
     3481 tttttgcctt tgatatcatt ggtcgcttgc aatttacaat gtgaacattc aaattctttg
     3541 aaatgattgt ttactttttt tatttcaaca taatgatgtc caaacaaaat gcaaatcaaa
     3601 tttttggaaa caattttatc ggctaagtag ttggttttca tctttattag cttttagttt
     3661 acaacaataa atttaattaa aaaacattga taacataaaa taatcgataa aaaacacttt
     3721 tttaagctat tattggttat ttatcttact taaaatcatc taaaatctac aatggttggt
     3781 tgcccaatgg tataatctgg aatgttaaaa tcatcgtttt ttaaagtgtt ggttttgaat
     3841 acatttgaat cctcaaaagg aattgaagga taatatgaaa aggaaacctg aaaacgatta
     3901 aagactaaat agtcgttatt tattaaaact ccaagtgtaa attttgaata caatttatta
     3961 tttaaaaaac tatcatgtga gtcacccaat aagccaaaag aggtattaat aaaaggacta
     4021 aagtgaaaac ctttccaatt accaggtgca tacgtttggg tttgaaaaac aacggataat
     4081 tttttagttc cgttaaataa tgggttggta aaaccatcaa aaccatttaa actatttaac
     4141 gaaattctat ctttaatact gttctctcta ttaattccaa caactaaaga tggtttaata
     4201 aattgtctga ttctccaatt tcctaacgac aataaactgg taaaatagtt tgcttcaatt
     4261 tttaaagtag tttcttgagt tttagtatca ttaaaaaagc ttcccaattc aatatttgta
     4321 cttaaatatc caaaaggata ataatgaccg tacgaaaatc gaccaccata ataattcctt
     4381 cttgtattgt ttttattttg aaatcctgcc gtaattgaat agacttttcc gaaaggtaca
     4441 tcttcaataa tatcaaaatt aaagaggtat ttatcttgga taaactttcg agtggttatc
     4501 ccaaaactaa ttaagtatag ttcttcacca ctcaaaaact gacttgggtc aatcataata
     4561 gaagcatttc gaatataatt tactttttta tacccaattg ttgttacaaa gttggtactt
     4621 ctaaaatcta ttgactttcc tttaaacagt tttgaggcat ttccaagcca aaaacttttg
     4681 gtttcatttt caaaaataat agtttcaaat tcgggaagaa ttgtaggtaa tgaatcagtg
     4741 tatttttggg tttcatagga aacaccacct gcatatttgg ttattggaga gaaaaaagtt
     4801 cgttctacaa ttaccctttt tgatacgtca ttattaaaag aactactata agaaaatgaa
     4861 gcgtcaatga aagtattctt aatattgttg aatgaatatt ttccgctata agaattctta
     4921 ttgtcgctaa attgtttgcc aaaatcattt tcaaattctt gacctaatcc aaaaaagttt
     4981 cgttcggtaa gttcaagatt cattctactt cctgaaatag caccatttgg aattaaactc
     5041 caagtgtcta aaacccttac tgaaacatct attgaatcta cattgttttc tattggaatt
     5101 gttttaataa gtacacttct aacaaagcgt tgtcttctta ataaacgttc ggattcttta
     5161 attagcaaag aatctaaagg ctggtttttt ttgaaaagta aattatttct aatcgtccat
     5221 tttttagatt tgatgtgaat tttattgcca aatatttcaa atgaattgtg aggttctcta
     5281 ttttcatctt cgtcggaata accaaatgga tcaagtgata cgatgtctat atttctaata
     5341 attttccctt cacttttacc gattttttca tctattttat ctcttagatt gttgtttgaa
     5401 ggtttgcttc tctgtgttga actaaatatt aatttatgga gaaactttct aaaattactt
     5461 ttattagaat acttttcgat tttttgatac attactttgg ttgaatccac cgctttcgtt
     5521 tttgttgtct gcgaatgccc gttttgaaaa acaaaaagaa aaaagagtaa acataatagc
     5581 agtttaggat tcatatacaa aagctaaaac tataaattag gttaaatata ccgaaatcgt
     5641 taacattaca atcgagaata aaaagagtat aaaaactaat ggcaatacaa attttaacca
     5701 tttgtcgtaa cctaccttaa caatggctaa tgaagcaaga atcaaaccag taggacttat
     5761 gaatgaaaat aatcccatgc cgtattgata acaatctact actgtttctc ttccaatgcc
     5821 aacagtatcg gcgagtggcg acataattgg catagttaaa acggccattc ctgatgatga
     5881 tggaatgaag aacgacaaac caccataaat tacaaaaagg acattggcaa acaatccttt
     5941 gttcattcct tcggtgatgt gacttgcata atataacatc gtatcgctta tcattccgtc
     6001 gtccattatt accgtaattc ctcttgcaat tccaatgata atggcaacac ttagtaactc
     6061 gccggcacct tttacaaagg tttctatgaa ttttgcttct ttgattcggg caacaaatcc
     6121 tatgagaatc gaacctacaa aaaagacagt tgtcatttca aggaaccacc attctaattt
     6181 ggctacacca taaatcatta ctacaaaaca caaaaggaaa ataaacagaa ttactctcaa
     6241 tcttgtagtt aattgaaggt ttgattcggt attgtttttg aaaagatttt caatttcttc
     6301 tttttggtca taaataatgg attgactggc atcatttttc actttcttgg cataacgcat
     6361 gatataaata atgcaaatta atgtgcctaa aacataggct acaaaacgag tgtttaatcc
     6421 agaagtccaa tttattcctg cagcgtttga agcaatgatg gcgctaaaag gattggtagt
     6481 tgaacacatt gttccaatac acgaaccaac ataaatggaa gctaacgcta ccattgcgtc
     6541 atattttgcg gctaagaaaa ctggaattaa aattggatag aaagcaatag tttcttcagc
     6601 aagaccaaag gttgttccgc caacagaaat taatgcggta acgagaataa ttagtatgta
     6661 ttcttttcct tttagcttga tggccagcca tgaaattcct gcgtcaaaag ctcctgtttc
     6721 gttcattaat ccgattaatc caccaataaa aagaactaga aaaataatat cagctccagc
     6781 aataattcct ttaataggcg attttactaa atcaaaaaaa ccttgtggtt tggcatcaac
     6841 ttcttcatag gtgtttggta tgtttatggg tttccaaata tcgcccgagg tgaatttttc
     6901 tattggaatt tttatgccta aactatctaa agttttggta gttccagcta cagtttttga
     6961 agcttcaccg tttttgatgt taaacatttt ggtttctttg tcatacgaaa gtcggttgta
     7021 ttttccagcg ggaacaaccc aagtaagtag ggtgaccatt gccgcaatta gtaataaaat
     7081 ggtttgtgct gacgggaatt ttattttttt cattgaaaca gaaatataaa atgccaagtt
     7141 aaacaattta atgaaatgaa ataaaaaaaa actcaaaagt gtttttttga ggtttggaga
     7201 tattctaaaa agttgcttta acttttctgt tctttgttga aatagactaa gtaatagtac
     7261 atttgttttt cttcatccca acctttttct acaaactttt cggcactttc gggattgatg
     7321 aaatctagtt ttatttgaat attggtgtcc aaattgataa cgttttttat tgattttcgt
     7381 gcatctgaaa cggcagcatt ggcaatcggg aaattcgtta catcttcaat actatacttt
     7441 tcgcctttat caactttata gtttttgaac tcgggaatca aatcaggatt gtttactact
     7501 tcgttaagga atttgctttc ttcaaactca tcgttcttag caaaaaagtt taccgaacgg
     7561 ttcataaaca ttacttcttc ttttttatct tcggcaggaa gcaccacgtc tttggcaaat
     7621 ccttgacaaa attttaaata ctttttagtg aagaaatttt catcttgaaa agcatctacc
     7681 gaaaggaaat gttccagcca ataacgagca tcataacgat tgctgtcaac agtcaaaatc
     7741 ttataacctt cttctttttt gtagttgaaa atcaaacaac ctttgtcttg tttgttcaaa
     7801 cttattcctt gttgaagaat catttcaagt gtagttcctt tttcttcaaa ctgcataaaa
     7861 tcagttttga tttcgctttt gaaaacacca atggcatcta ccacattatt gtcaatagaa
     7921 atgttagtga tataagcaac atagacttca ccattcttga tgtgtggatg gttggattgt
     7981 tcaaaaagat gtgatgttat cttttttgaa atggaatgca cgtcacttgg gttggtgaaa
     8041 acttccgttg ccaaattgta catttcgtta taatctaaat ctacatcgtg tgcaaactgg
     8101 aaatagtttt cttctttttc tctaaatggt ttgaaaaaat attcttttaa taatggaacg
     8161 atttcgtcat tcaagttata cggttgttcc gaaaggaaaa ttgcttcgtt tctgcttttg
     8221 ttcccaactc ggtgtatgga caaactctcg atatgagcgt tgaaaagatt tatcattttt
     8281 taaaatggtt taaagttgtt taatttggtt taaagttgtt taagatttga tgaatttagt
     8341 tcctttgaaa tcatttttac ttaggtaaac cataaaatta tgaattttaa tattcagttt
     8401 ttcgtagtct agttttaaaa tatctaattt ttttcatcta tataatcatt gtcaaaaact
     8461 ctgtataatt gtaatcgtgt ttcgcctgct gaacctttag cgattgaaag aaaattattg
     8521 aattcagcat ttccgcttcg ttcaaatcct tcagctatat tgtccattac agaacctgaa
     8581 gaagctttta tttggtctct taatctaaaa tcggttttta aatcagttgt tttagaaatt
     8641 ttaattattt cttgtctaag tcttctagct tctatccaaa tatctaattc ttcaaatctt
     8701 ttaatagtag ccataacaat aattttaaac tattttacac caaattaaac aactttaaac
     8761 gatatattta attccaattt tcctcgaatc catagtcttc aaaactatcg tcgctaccaa
     8821 aggcatctag gtcgtcttcg tctatgtcat cttcaaattc ggaatagtaa tcgtcttgat
     8881 tttcggcttc aaattgtttt tcgatggctt ctgctggcag ttcgccgtga gagaataata
     8941 aggctggata ggtttctcca attacgggtt cttcaacggc agctagttca actaggaatg
     9001 tccacatgtt gatgaaatca tatacataga tgattttggt ttgttctaaa ttcaacacat
     9061 catttagtgt ataatctgcc attgtttttt gttcaccaag tacatcgcct gagtcaaaca
     9121 ttggaatttc atcttcttga ttccaagtgt cgtcacaagt atagaacgat gctacttcca
     9181 taccgtcgaa accaaaagcg ttaacgatgg cgttgtgcaa atcttctaaa gtgtcttctt
     9241 gatgaatggc tatatctcta aaaacatcgt cttcggcatc aagaatagct cggaacttat
     9301 aaatcataat cgtaattttt ttgagctaac aaaggtaaaa tattaatctc aaaatgaaat
     9361 tacgaaaatg gatttgttga aaagtatttt taaaaaaaaa cctagccgaa actaggtaaa
     9421 atttttattc tgttttgatt gaatttatca agatgttgag cgtttcgcca aatggtttca
     9481 ttttagcata aacatcttgt tttgaacctt tgttttgtgc tacttcgtct aaagcagctg
     9541 cttctgattt taattttcca accatttctg ttattgctgg agtaactaat tctgcaggaa
     9601 tactttttgt atcaagtttt tcggaatact tttttaataa agtggtgtat tgtcctatta
     9661 cttcaaagtt tccttcgtta gctacatagg ctactttagt atatatttgg tttacagtat
     9721 ttaaatcttc ccatttatca aggatttttt gagcattcat ttgagaaaaa ctcgtaagga
     9781 ctaatagaat tactattgat tttattgttt tcataattat ttttttaatg gtttctttat
     9841 gcaatactac taaatatttt tgaaaaattt agcattgtgt ttttgttttt tagttaaaac
     9901 ttttagaata gacttctaat tattctttat tgcccaaagt ccaaactagt gctttttctt
     9961 ttgtggctct aaaaatagtt gaatgttttt cgttgggttc gtcagagtag gtccaagtta
    10021 tgttggatgg tgctttagaa tcgaacgttt ttgccaactt ggtagtaaat tgcgaaatgt
    10081 cttcggcacc agaaccggca aaccaaactt ttgtgttttt agtagtaaag ttttgtaaat
    10141 attttgctcc atcacgttct aattcatgat tgttccacca aatggatgga tcgaaagcga
    10201 tgtagaaatc aaaggtttca gtttttttga aaaaagtttc cattacaaat aatcctgcca
    10261 atgattcgcc gatgatgcct ttttgattgg ttgttcggta tcttttattg atttcgggca
    10321 ttaattcgtc ggtaatgaaa tctctaaatt tatcggcacc accaattatt ggtgctactt
    10381 ctttgtcttt ttctaccatc gtaaaaggtg ttaaatctct gcgtcgttca gtattttcga
    10441 tgccaacgag catcattggt ggtatgcttt tgtttttaat tagttttgct aaagtgttgg
    10501 ctatatgtgg aaaatcttct ttcaaaccgc cgtcgggcat gtataaaaca ggaatagagt
    10561 cggtactttc ggcatacatt ggtggtgtcc aaacgttaat tgttcttttt tcgcctactt
    10621 tttttgactg tatggtaaat tcgtcatgtt ttgggatagg atcgttattt tgttgtttat
    10681 tggcacaact ggttaaagtc atgataacta ttaaaaaaca gatagttttt ttcatcctct
    10741 attaggtttg ttgatttata aaaagtaaaa ttagcgctat tttggataaa agttttatag
    10801 taaaaaaata aaaacattag acgaccattt tcaatacaaa aacaattaat gctgtaattc
    10861 caaagctaat taataataac agtttccagt agtaagttct tgaaccatac gatttccaca
    10921 ttttttcacc aatttcggtt tgatattttt ttagagttac ttttgataat agaaaagtag
    10981 tcgttatgaa aataataatt gaaactaacc aaaatgcatt catgacaata gctatttatt
    11041 ttatgttttt taatttaaaa actaaacttc taaggtacga ttttttttaa ttctatatta
    11101 ttttttcaga tattattttt ttaaaagtta tcaattttcc attaatatgt ttagatttga
    11161 aaaattggaa tgtagtaaac tttggtgttc aaagtgtttg ttttacagtg tttttaaaaa
    11221 aaataaatct ctaatgggaa catttgttat cagtaagcgg gttaatggtg attacaaatt
    11281 tatttttgca actagaaaag ggaaaactat ttttacgagt attggttgta aaaataaatc
    11341 ggattgcgag aaaatcatag ctgctattca aggaaatatt gaacaattta cgttaactag
    11401 aagtaaaaat gctgctggaa aatacttttt ccgattggca aaagatgggt tagtgttggc
    11461 taccagtagg aagttttcta ccgagtttat gtttcaaaaa ggaatagaca acatcaaaga
    11521 acatttgccc aattcggaaa cgttggattt ctctgaaaat gagtttgttt ttccagattc
    11581 ggaagcggtt tttgtggagg aagagtagtg gttttttttt tgccacagat tggacggatt
    11641 aacacagatt ttttttgtgg tggttttttt gccacagatt acacggatta gggcagattt
    11701 tttttgtggt ggtttttttt tgccacaaat tacacagatt agcacaaatt attttttgtg
    11761 ttggtttttt ttttgccaca aattacacgg attagggcag attttttttg tgatggtttt
    11821 ttttgccaca gattggacgg attagcacag atttttaact caaaatgtcg tttgagattt
    11881 tgaagggtat ttgttggctt ggggttattg atactaatac ttaaacgcgt tttagtaatt
    11941 cagctagtgt tgctacaaat ctaatcaata aaaaattaat gttccataga gcctataatc
    12001 cgagagaaag ttgtaatatt cattttcttc ttctgctttc atcttgaata tatgtttcta
    12061 atagcatttt tgaagttttt gcgatgtttg ttaaattagc gaatgacatc ttgttgtttc
    12121 aacgcgttgc taactttgga ttgctctagt tttttgtttt ttttatcagt ttatacatgc
    12181 ataaacccta atattcatgc tttagtggtc gtagctgatg actcgtgata tttgccattt
    12241 gtcattggtt ttcttccaaa gcattgcgaa tttaaaagtg ccaatttctt cttttccgtt
    12301 ttcgatgtgt cgaaattgat gcacgcctat ttggatggcg ccataatctt taataggatg
    12361 cacttctaaa ctgcctttga ctagttgcct ggttaattta tcttcttttt tgaaggtgtt
    12421 ttcaaggttt tcaaaaacgg tttcatacgg aattagtcct ccgttgtctt gataccattc
    12481 taaatcttct gtaaatagcg gtttgaattg cgccatgttt tggttattga aggcatcaaa
    12541 cagcagcgtg tccatttttt caatctcctt aaataattcg ggtgttgttg gtgctacttt
    12601 ttttcttgtg ctacaacttg cgtgctaaag gatagtgtaa gcagaaatac taagttgcgg
    12661 atatattggt gtaaacccat tgtaattttg tttaagtgct aaatataggg attttattat
    12721 ttgaatggca ctgattacac tattgcaaac tactatttag gatactattt gatttgaatt
    12781 agtcataagc gggattaata actatcctta actttttctt ttttcgcgtt cttgtttata
    12841 gcggtctaag acttggtttt tttcgttttc ggttaaatca tcccacttat tatcaatgaa
    12901 ttgtatcagt tgatcgtagt cattggccat ttcttctatt ttggacatgg catctttgcg
    12961 atgcttcata ctgtcttgta gaagtaacat gtttactatt attttttctt cttctggtga
    13021 cataagcact gttttttaat gatactatat tactaacgaa ttaattatgg ttttattgtg
    13081 tattgttatt tttttttaaa tgcatttttg taaaataaaa tctgtttagt ttaacaactc
    13141 tgttatttgt ttttttaaat cattaagggc attaagatta ttaagttttt ttgggttttg
    13201 ggaatttttt tgccacaaat tacacagatt agcacaaatt tttaactcaa aatcatgttt
    13261 gagattttga agggtatttg ttgggttggg gttattgata ctaaaactta aatgcgtttt
    13321 agtaattcag ctagtgttgc tacaaatcca atcaataaga taaataatgc tccatagagt
    13381 ctataatccg agagaaagtt gtaatattca ttttcttctg ctgctttttt tctatttgaa
    13441 attatccatt tacttattag aaacccaatt ataatggaaa caatactata aaaattagtt
    13501 gtaaatctat tttagaatta agtataaata tgtaaactta ttttaggacg agacattgtt
    13561 agtggagaca gattgtaaat aagtgctaac gggttctcga tacactttta aaagtaaaaa
    13621 ctgacgtttg tactttataa aaatcactag aacggacggt gctgaaatat cataggatgg
    13681 ggattaattt cgcttttttt gctatggtct tgggataaaa attactctaa cggacgaggg
    13741 tttttaattt ttgggtaact gacaggtctt caaagaaaac atacaggcgt tcaaagagaa
    13801 agttgggttg ttagagtttc ccctgcagcg cttctatctg gtctctaaac ttggcggcgt
    13861 ggatgaaatc taaatctttg gcggcttttt ccatcgcttt gcgcagctcg cggattttct
    13921 tttcaatctc gggttttgag aggtaaaggc tttcgggttc ggcggcgtct agtgcttttt
    13981 cgtggtaata gttggtagaa atagagttgc cgctcaaggc attgcccaaa ctcttgttga
    14041 gtgctttggg ttccaatttg tgtagcgtgt tgtaggcagt ttgtttttcg cggcggtagt
    14101 tggtttcgtc tattgttttt tgcatgctgt tggtaatctt atcggcatac atgatggctt
    14161 ttccgtttac atttcgcgcg gcacgaccaa cggtttgcgt caatgagcga tggctgcgca
    14221 gaaagccttc tttatcggca tcgagaatag ccactagtga aacttcgggt aaatctaggc
    14281 cttcacgcag caagtttacg ccaatcaaga catcaaacaa gcctttgcgc aaatcttgca
    14341 tgatttctac gcgttctaaa gtatcgacat ctgagtgaat ataacgacag cgaattgcta
    14401 ctttggttaa gtacttggtc agttcttcgg ccattcgttt ggttaacgtg gtcactaaaa
    14461 cgcgttcgtc ggcttcacag cgcagttgta tttcctcaat caaatcgtct atttggttca
    14521 ggcttgggcg cacttcgata attgggtcta ataatcccgt tgggcgaatg acttgctcta
    14581 cataaacgcc atcgcttttc tgtaattcat aatcagcagg cgttgccgat acatacaaga
    14641 cttggttctg catgctttca aattcctcaa acttcaacgg acggttgtcc atcgcggcag
    14701 gcaatcgaaa accatattcg actaggtttt cttttcggct tctatctcca ccatacatag
    14761 catggacttg cgaaattgtt acgtgactct catcgaccac catcaaaaag tcatcgggaa
    14821 aataatctaa caaacagaat ggtcgcgtgc cgggcaatcg tctatccaaa tagcgcgagt
    14881 agttttcaat ccccgaacaa taaccgagtt ctttaatcat ttctaaatca aagttggtgc
    14941 gttcctcaag gcgtttggct tcgagatgtt tgccaatttc tttaaaataa tcgacttgct
    15001 tgaccaaatc ttgctgaatg tcccaaatgg cagcttgcaa gacatcgggc gaagtcacaa
    15061 acatattggc cggatagatg ttcagctttt cataccgctc aatcactttg gtagagcgat
    15121 ggtcaaacgc ttcaatttct tcaatctcat cgccaaaaaa atgcacccga aacgggtcat
    15181 cggcataact cggaaaaata tctacggtgt caccttttat tctaaatgtt cctggtaaaa
    15241 attcggcttc cgtcctcgaa tacaaacttt gcaccaagcg atgcaacaat ttagttcgcg
    15301 aaatgacctc acccttagca atagaaacca cattcttctg aaactccacc ggatttccaa
    15361 taccatataa gcacgacacc gaagaaataa ctacaatgtc acgacgacca gaaagtaggg
    15421 cagacgtggt actcaaacgc aacttttcta attcttcatt aatcgataaa tctttttcga
    15481 tataggttcc cgttacagga atgaaggctt cgggttggta atagtcataa taggaaacga
    15541 aatactgcac cgaattattg ggaagaaact gtttaaactc ggaatacaac tgcgccgcaa
    15601 gggttttgtt gtgcgccaaa atcaaagtag gtttttccac attttgaatc acattggcta
    15661 cggtaaacgt cttccctgaa ccagtaacgc caagtagggt ttggtacttc tcgccgttaa
    15721 gaattccgtt agagagtttc tctatggctt gtggctggtc gcctgtaggt ttgtaatcgg
    15781 atacgacttg gaatttcata atttataaat tcagtgtagc agggttttgt ctggtatccc
    15841 aaacggataa aatgattatg tgactttcgt taacttcata gaaaacgata aaattttcaa
    15901 tgagtaaacc tctaacgttt atggtatcgg tttttttacc aataaatggt tgttgaacta
    15961 gaagtttaat ttctgattga atagttctta gtagtttttg agaataggtc ttacttttat
    16021 ttctgagatt gtagtattcg agaatgaatt ttaattctat tttagccgtt gttgtccaaa
    16081 taattattct ttcagccatt tctccatttc ttcgttaacc gtatcattgg tttgataatt
    16141 cccttctaaa tactcttttc tagatttttg aatactggat ttctgattgt ctgaaagcat
    16201 tagaatattt tcagatttac tatccaaaat tgtattaatc gcttttaaat aagcttcgtc
    16261 attgatttca tttatcttag aaatcagtat cttttttaat tcaattgtat tcataatata
    16321 taagtttata caaatttaat taaaataatc atcattcgga aaacttccaa ttcttttgct
    16381 ggtcttccga aagaaatgtc caagccacca tgcgacttat cttttgacct tgcgccatat
    16441 cgatggtttt gatttctact gcgcccactt tgttcaataa acgatagatg cttttcaaat
    16501 tatctttctt agacactaaa gtagtgaacc aaaagacttg caatggatat ttaacgcttt
    16561 cgtatatcat ttgggtgata aaagcaaact cgccaccagc acaccaaagt tcgtgttgct
    16621 ttccaccaaa gtttaagatg gttttatcgg ttttggattt cgataggtta tttacttttc
    16681 gcaacgcagc tttatgggca tcttctgctg agttgtgaaa cggcggattg cagatggtaa
    16741 aggcaaattt atcttcgggt aggatgatgt ttttaaagat gaagcgcggt tcggtttgta
    16801 attgcaaact tatggcatcg ataagctttg ggttgttagc aataatcttt ttgcagtttt
    16861 gcagggcatt ttcatcaata tcgcaaccaa caaaactcca atcataagct gcatttccga
    16921 taatcgggta gatgcaattg gcaccgatac caacatctaa accagtcacg gcttcacctt
    16981 tgggaattat gccatcatta gaatcggcca aaaggtctgc gatataatga atataatcgg
    17041 cacgtccggg aataggcgga caaagatatc ccattggaat atcccagttt tgaatgttgt
    17101 attctgaaat aagaatggcg ttgtttaagg ctttcactgc ttcgggattg ctaaagtcta
    17161 ttgattggtt atcgtgttcg ttaaccaaaa caaattgctt cagcttagga ctatctatta
    17221 ttaattgatc aaagtcatat cgaaaccgat gtggatttcg aagatgaaaa ttgctttttt
    17281 ctgagtatgt attttgtatc tttttcattt aatcccaaca ttccattagt aataagtcgc
    17341 cttcttgatg ttctatttta actatgccat cttcggaaac atgattacta cttccggtac
    17401 gatatccaat ggttagctct tcgttattca aaggataaaa aaggttttga gtggtaattc
    17461 cagttacttt accaactgga attaacgaaa taatggtgtt tgctgtgtac catttttcga
    17521 acttttttgg taacaaaaat acctttgaat gatcgtctaa aagtacaatt tttaattgat
    17581 tgcggtaagc tataatgttg gtaatgtttg taatagtatg atcagcacgt tttccggttg
    17641 cccaaatgac attcaccgct ttatgacctt tttcaatgag ataatcgaag gctttttcta
    17701 aatctgtttt atcttgattt ggtgtatgta caatttcgat agggtattgt cgttctttaa
    17761 ttttttcagt atcaaagttt ctgtcgaaat cgcctaaaag tacatcgact ttgatgttca
    17821 aatccagtac gcgttcaatt gcagaatcta aaacaatcac atacggcgac cattctaata
    17881 attgaccaag aagttcgttg ctacaactgg caccattggc aataattaaa gcaggttctt
    17941 ggtcgtcgcg aacaatgtga tgtgatgaca ttaggtttta ttcaatgatg tattctttga
    18001 tttcagtttt actcaacctg aaatatccca aggcaaagtt atcaaaatta gtttgattga
    18061 cgatgttacc tctaatggtt gatgttggcg tagaaaaagg accagcattt ccaccaccag
    18121 cttgagaaag taagatattc atgtagttgt agtaatttaa agtgatgcca ttgtttgtga
    18181 attttattgt atcacctgtt tttaaatctt cactaaaata caaaccaaac atttcattat
    18241 tttgaaaaaa acggtcttca agaacaccaa actcaggaat tactttgtag ggatcattta
    18301 tttttagaaa atagtgattg gtttcattgg gaatatcatc aaaaacaaat ttgacctcag
    18361 tagcattgcc taaaatgcca ccatcatttt tttgttctac tcttttcaca attggggtat
    18421 tcaataattt ttcagttgaa gtgtaaactt catttttata cattacagtt aatgtgtaag
    18481 tttcatccaa aactggatta aaatcagcac aattatagat tccaggttct gtatcttgca
    18541 caaaattaaa ttgttgattg ctactgttag ttataaaaac aatggcatct gtaaccggtg
    18601 gaactgtgtt tgaaaaataa tcagttgttg tggagagttt tattttttgt tggtttccaa
    18661 ctgtaccgta aacccagtta attgaagctt caatgactaa tcgtggtgct gcagtatcta
    18721 gaccaacatc aataactttt tcgcaagaaa gaaatataaa agaaactaga attaagatat
    18781 attttgatgt tttcatgatt taaaatttaa tgttgtaggt tgcactaggt accactccaa
    18841 aaatagataa ttggacggct tcattttgtg ctgttttatc attttgtcta aacgtaatcg
    18901 acgctgcgtt ataacggtta taaagattgt aaatcccgaa aacccattca gattgccact
    18961 ttctgccttt gttttttcgt ggtgttaacg ttgccgaaac atctattcga tgataggttg
    19021 gtaaattgtt ttcatttcgg gtgctgtaac ttggtacagt aattccttcg tattgatatt
    19081 gtccgtttgg aaaagttaca ggttgacctg tttgcaaagt aaatgttcct ccaaaacgcc
    19141 atttttcatt aagtttataa ctaccaacca acgatatgtt atgtgtttta tcaaagccag
    19201 ttttatacca atcaccattg ttaattccgg tttcaattga agttctgcct tctacttgtt
    19261 gctctgttct cgataacgta taagataacc aacctgaaaa tcttcccgca ttttttctaa
    19321 aatatacttc tagcccatat gcacgcgctt ttccgttgag tattactctt tctatggctt
    19381 cgttggcaat caaattagca ccgtcaatat aatcaactcg gtttttgatt ttcttgtaaa
    19441 aagtttctac ttctaatgaa tagtcgtcgt ctttgaagtt ttgaaaatag cccaaagcta
    19501 cctggtctaa taactgtggt tttaaaaaat tatcacttgg agcccaaaca tccaaaggag
    19561 ttggcgattg tgtattggaa attaaatgta aatattgtga cattctgtta taactagctt
    19621 tgactgattg atgatcattg atagcataag aaatcgctaa tcgaggctca aagttttcaa
    19681 aatgtgcaat ggtttgattt ttaccatagg aaactgttcc tgttggttcg gcactttcat
    19741 aaatctggaa attaggattg aaaattaccg cttgattgtt ttcataagta ttcaaagttt
    19801 gttgtcctaa tcgaagaaag ttactcaatc gaaagccata actgatggtt atttttttgg
    19861 aaatatcttg ttcaacatca aaataaacgg cattttcaag tgcatatttt ttatctaatt
    19921 gttctggatt tatatcagaa cctgttccag ttggttcaat ttttcccgga ttaaaatcat
    19981 aataattact ctgaaaaccg taagtcaatt ttaatttgtt attcaaataa tgtttgaaat
    20041 cgtatttgat attgtagttt ttaatgccac tattccaatt gaaaccaata aattctagag
    20101 tcaatccata ataataatca gaatagatta aagaaagatt ggaaaataat ttatcagagt
    20161 acaaatgatt ccatctaaag ttgacaactg catttccata ggtgtttaca aaattttcgt
    20221 ttaaagaaaa cacatctcga ccaaaatatc ctgataagta aatgttgtta ttttggttta
    20281 aattataact aagtttcgtg tttaaatcat aaaaataagc agaatttgaa ttgttagcta
    20341 atttcaaaaa aagatgcgca taggttccac gaccagcaac aacaaaagaa ccttttcctt
    20401 ttttgattgg accttcggct aatagcctac tagaaattaa tccaatacca ccattcatgt
    20461 gaaagctatc tttgtttcct tctttttgat aaatgtcta
//
LOCUS       DAOOBH010000029        20399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_3471_length_20399_cov_7.392696, whole genome shotgun sequence.
ACCESSION   DAOOBH010000029 DAOOBH010000000
VERSION     DAOOBH010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 20399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20399
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3471_length_20399_cov_7.392696"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /gene="rpsI"
                     /locus_tag="PK218_04940"
     CDS             <1..115
                     /gene="rpsI"
                     /locus_tag="PK218_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPW97885.1"
                     /translation="NRAILKPEGLLTRDPRMVERKKFGQKKARKKFQFSKR"
     gene            292..1077
                     /gene="rpsB"
                     /locus_tag="PK218_04945"
     CDS             292..1077
                     /gene="rpsB"
                     /locus_tag="PK218_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPW97886.1"
                     /translation="MANKIEVKDLLEAGVHFGHMTRKWDPNMAPYIYMERNGIHIINL
                     YKTAAKIEEANEALKKIAASGRKVLFVATKKQAKDIVAEKAAAANMPYITERWPGGML
                     TNFVTIRKAVKKMAMIDKMKKDGTFMTLSKKERLQVDRLRAKLEKNLGSIADMSRLPA
                     ALFVVDIKAEHIAVKEAQKLNIPVFAMVDTNSDPREVDYVIPSNDDASKSIDKILSLV
                     TTAIIDGLANRTSDKDAEVETTEVEATTEVETPVATTETSTEE"
     gene            1215..2039
                     /gene="tsf"
                     /locus_tag="PK218_04950"
     CDS             1215..2039
                     /gene="tsf"
                     /locus_tag="PK218_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPW97887.1"
                     /translation="MATITAADVNKLRTITGAGMMDCKKALVEADGDFDLAIENLRKK
                     GQKVAANRSDRESTEGAAIAVVNADKTSGVAITLNCETDFVGKNEGFVKLATELANQA
                     LNYDTKEAFLASDFNGITVAEKLIEQTGVIGEKIEIASFERLNGTFVGSYIHAGNKIA
                     TLVSLSANVAGAEEASRNVAMQAAAMSPIALNEDGVDAAIIEKEIEIAKDQLRAEGKP
                     ETMLDNIAKGKLNRFFKDNTLVNQDYIKDSSMSVAAYVKTIDSGLTVTGFSRVALG"
     gene            complement(2142..3695)
                     /locus_tag="PK218_04955"
     CDS             complement(2142..3695)
                     /locus_tag="PK218_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97888.1"
                     /translation="MTVKVYKKMLLGVLTILLVVFVLNILLNGWIKHQLPTIINEKND
                     TSYFITYKKLEVSLLRRCIFAQNIVVVPKSSLKDNISKKGIYANIQTIEIKDINVWNL
                     IFSNKIKASTIGIKSPNIIVYDNNNHYSVRNSVVAPFEKIIKVSNILLSNGNLKVLSK
                     NEAVPILSVNNINFNLEGIKITEEILNKKIPLEYENYTISCDSLFYHPNSFYNLKTNK
                     VNITKNTTQINNFEMIPAFSRREFVSKLTNEKDIYTLLCKSISLKNSNWGFRGDDFFF
                     HCNLITLDNVNANIYRSKEPTDDLSKKHLYNKLLRDLKFDLQVDTLKVQNSKLVYEEE
                     KNSEIGAGVLRFNDFNLTATSLNSGFKKEKLSDVKIAIHCKFMKNSPFTVNWSFNVLD
                     KRDGFKIQGKLGNFEAQQINAFTKPYMNVTTKGILDEVRFNFSGNDLKSSGQFAVQYD
                     DLKFTVYQKDARKKKNKVLSFIANIFVKDDTKDKITETTIEVDRNPEKSFYNFLWISV
                     AEGLKKILL"
     gene            3941..5275
                     /locus_tag="PK218_04960"
     CDS             3941..5275
                     /locus_tag="PK218_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HPW97889.1"
                     /translation="MKYFKLTMLLLSVLTLTNCSSDPESYSNPQGNPPPGATLPTLAT
                     ADVTNISFTTAVSGGIITSDGGSPIIAKGIVWSLAHNPTTLLGTKTNEGEGSDVYTSN
                     MTGLESNKTYYVRAYARNGKGVSYGQEIEFNTLVDVNSLPAVTTAQATNVTTNSVTIG
                     GNVTNSGISPVTARGVVWTLSPTVPPTVLVNLGKTINGVGLGTFVDNLSNLTPNITYY
                     VRAYATSLYGTNYGEVVSFTTSPLLYTIGTSVTDINGNVYGSVKLNNQQWATKNLSVT
                     KYRNGDVIPQVQDATQWAALTTGAWCYYSYQSANGTVYGKLYNWYAVNDARGLAPTGW
                     HIPTNEELISLIDFLGGTEAAGGLLKETGTTHWEAPNTGAINSSGIKALPGGYCLADG
                     TFSNLGTKGYWWIADSYNPSSAWCAGLYNDTKTITRAPIDKRQGFSVRIVKN"
     gene            5365..9042
                     /locus_tag="PK218_04965"
     CDS             5365..9042
                     /locus_tag="PK218_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPW97890.1"
                     /translation="MRNYILSLLLVCLTTSAQEKTLFWEISGNGLTKKSYLYGTMHVS
                     DKVSYHLSDAFFKNLLNADMVANESDPETWNDLTDVVKEPNFDAKANFYTEFYKSVVN
                     KNDVKAVFNITNNFFNNLLSSIGGETSDYQENTVLDMFIYQTGRKYNKKNVGLEDAKG
                     SLIPILKIKEEDAKPKDENLQLLVKLMKNRPFNEVLKQYYREKDIVMLDSIYKLMMSK
                     KAHDVLITIRNENMTKSIDSLVKKGSLFSAVGAAHLAGNEGIIALLQKRGYTVKPIVD
                     VFSESGNKQKKNIEDYFPNPGFVTTSTSDGMLTMPLTKNVFEEFATIGAPDFTNGGVI
                     NIRRVPLNYYLNKKENLYNIKALDSLFFENIPGTILDKKMFEVDNISGYDVKNITKTG
                     SNQRYKFYVTPLEIIGVSMTGVGNYVRQFENEIFDNIKLKSSKETWETMMPNKGGFSV
                     ELPSFYSAYGNNKSYEDLEIQAYDPATKSYYFLTEKTNNDTDFLENSEFEQHQIQYEF
                     YLQHDAAIENSNYDKVSKSLETSSKIGDKNIRLKTIINGDKYYLLGTINASESDANRF
                     FSSFKTTSFIYKNETSVFTDSINKYRIEIPKKLNDKLFLNLGFKEKDTKNQFEEKTKY
                     ASYRSETRKNVTLLYNKFSKYESIATIDSLRINFREYFLKEDSNFKKDTYDDYDYDHV
                     SSLLSVTYKHKGITPSKWDDYIQKEEEKYEMLSETFDYDKEKNIYVLNNIVSRQNTLQ
                     AIKYKTVFINDSYYMLSTLINRNYKNDDAFIEKTFNSIEPYGENLNHNYSIFDDKINL
                     FINDANSESDTIRYSAMNSVYDLDITKKDLPAIKTFIETFTFKDSETSAIQSLMKKIG
                     LIQEESVIPFLDNLYRKEDVKTTIQLGVIQALINQKTKKSYQKIIELLEYDLPISDNE
                     YDITNLFENFEDDLEHSKELFPKIFQFYSVKEYNEPIISFCNLLLDQNLVEGNKIKSF
                     KKMILTNAKLEYKRVASWKEKNKPDEETIASDDVVEDYDYNANSANVNSLKNYIGLLY
                     EFKNDTAINDYFKKVKKLNLPEIDLEFTRLGIVNNRISNEEIQAALNNEENRFVVINL
                     LLSKNKDVAIKLSDDEIAKSALLNLKGLTVKDTINYIDKKIVEQAGKQTVYYFYKAEK
                     KSYSYDIAKKIIYTMAFVLDNNKINPLAFKMFPDEFLEDDDEMNKTLDLIIKKSMNED
                     HKRASFEKVDPNKNRYFYNDY"
     gene            complement(9114..11231)
                     /locus_tag="PK218_04970"
     CDS             complement(9114..11231)
                     /locus_tag="PK218_04970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPW97891.1"
                     /translation="MKKIMFNLILIFPMISMNAQKNDNPFLNFWSGPYGGIPAFDQYK
                     LKDLKPAIENAINEKLSEITTIANNKKPPTFENTIVAMEKAGKKLSQIYAVFGIYSSN
                     MNTPEFTPIETEMSPKFSEISNKIYQNEKLFQRIETVYNSPEKNKLTPEQQRLVWLYY
                     SNFVREGAKATKKSKVRIAQINQELAGLFTKFSQNLLNDENENSISFTEKNELAGLPA
                     DLINGYLTKSKELGRNDLGTVANTRSSVEPFLTYSSFRPLRKMVWELFVNRGDGDNQN
                     DNKTTLVQILKLRAEKAKLLGFKTFADWSLSNTMAQKPEKTIALMESVWKPAVEKVHK
                     DVAEMQKIVEAEGGNFKIEPWDYRYYAEKVRKEKYDLDENDVKPYLQLEKLREGMFWV
                     AGEIFKLNFKQIFTLPVYHPDVRVFEVTNKTSGKVVGLWYFDPYARTGKRSGAWMNAL
                     REQSRVNGDVITIVTNNCNFIKGKENEPILISWEDASTLFHEFGHALHGLCSNVTYPS
                     LSGTNTPRDYVEFPSQVLERWLATPQVLNQFALHYKTGKPMPMALVERIEKASTFNEG
                     FSTVETISSALVDMKLHLLENPDIDPSKYEKEVLKSLDMPSEIVMRHRIPQFGHIFSG
                     DEYAAGYYGYLWADVISADATEAFTETKDGLYNKVVAKRLLDDIFSIGNTIDSEIAYK
                     KFRGKAATSDALMRARNFPIEKK"
     gene            11345..11575
                     /locus_tag="PK218_04975"
     CDS             11345..11575
                     /locus_tag="PK218_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HPW97892.1"
                     /translation="MQNNNPLHGITLQKIVTDLVAFYGFDTLSELIKINCFKSNPSVK
                     SSLTFLRKTDWARKQVEDLYIKTLPKINKNGN"
     gene            11565..12125
                     /locus_tag="PK218_04980"
     CDS             11565..12125
                     /locus_tag="PK218_04980"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPW97893.1"
                     /translation="METKNRCAWCEKDDLYREYHDNEWGKPVYDDDKLFEFLVLETFQ
                     AGLSWHMILKKRENFRKAFANFDYNLIAAFDEYEVQELLKDVGIIRNKLKIKATITNA
                     IAFKKVQTEFGSFSSYIWSFVSGKPIDNNPKTLKDVPASTSLSDIISKDLKKKGFKFV
                     GTTVVYAHMQATGMVNDHVEDCWTRS"
     gene            12195..12923
                     /locus_tag="PK218_04985"
     CDS             12195..12923
                     /locus_tag="PK218_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HPW97894.1"
                     /translation="MFRHKGSRRTFSHNIRLASTLSFVAGIVNIAGVLSVATLTTNVT
                     GHFAFFAEEISLKNYTKALNIIVYIFFFLLGAFTCNLLVEIVSRKKPRMAHALPMFTE
                     IVILTSVGFSTDFNPNYIAYALLFAMGLQNALVTNISQSVVRTTHLTGLFTDLGIELS
                     QLFFYKKETEMKRLTRSIFLRISIILFFFIGGVLGGLLFKSFQLKILLFAVVILVVAL
                     FLDNAIIYYNLTKRKIKIMTSKSS"
     gene            complement(12901..13536)
                     /gene="aat"
                     /locus_tag="PK218_04990"
     CDS             complement(12901..13536)
                     /gene="aat"
                     /locus_tag="PK218_04990"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPW97895.1"
                     /translation="MYYVTKKLYFPPVEEASYEGILAIGGDLSTERLLLAYRNGIFPW
                     FNAEEPILWWSPSERMVVAPQLYKICKSTRNLLNQKKFKVTFNHDFRAVITNCQQIER
                     KDQDGTWITNDLIEAYVKLHEMGFAQSVEVWQNDELVGGLYGVDLGHVFCGESMFSKV
                     SNASKIAFVTLINHLKEKNYKLLDCQVHNDHLEKLGAFEISRETFMKILKS"
     gene            complement(13633..14007)
                     /locus_tag="PK218_04995"
     CDS             complement(13633..14007)
                     /locus_tag="PK218_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HPW97896.1"
                     /translation="MEVTGKIRVVGAEQQVTATFKKRELVVTTEEQYPQYISINFVQD
                     KCDLLNNYAVGDAVKVSINLRGREWVNPQGETKYFNDIQGWRVEKLQAEAPAAGMPPM
                     PPAEAFAPATNFNEEEHDDLPF"
     gene            complement(14010..14885)
                     /locus_tag="PK218_05000"
     CDS             complement(14010..14885)
                     /locus_tag="PK218_05000"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPW97897.1"
                     /translation="MLSFEPHTLSPAQLQGYLQSAVAPRPIAFASTIDEKGRPNLSPF
                     SFFNVFSSNPPILVFSPARRVRNNTTKHTLENCIATKQVVINVVNYDIVQQASLSSTE
                     YLDGVNEFLKSGLTMLPSDMIKPYRVAESPVQLECKVNEIIALGDQGGAGNLIICEVV
                     KIHINEAILDDKGMIDQSKIDLVARLGGNWYSRSNQGLFEVEKPLSTLGIGVDSIPEF
                     IRESTVFDGNDLGKLGNVEALPSEEEITIFVQNNTAVKGVLSSDDTDKIHLKAKEYLN
                     NNDVLSAWKVLLAKL"
     gene            complement(14998..15159)
                     /locus_tag="PK218_05005"
     CDS             complement(14998..15159)
                     /locus_tag="PK218_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97898.1"
                     /translation="MKRQEESNGKQFVNHGSIFESNGYIFEAIGSIIERHGSPFEGHG
                     RPFEGYKAF"
     gene            15328..16488
                     /locus_tag="PK218_05010"
     CDS             15328..16488
                     /locus_tag="PK218_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW97899.1"
                     /translation="MQFSERRNSTRWIIIITSFVIVTLILWNTYSFFQIFKNEERIKM
                     ELWGESMKTLLNADPETTEVELPRQIMSNNTTIPIVLVENGVIINQTNIDEEITKNKT
                     KLNAFLEKLKKENEPIKIEYDKNKYQYLYYGNSSLLNKLKYYPVALLLIIFLFGAVVY
                     NFYRSTKMATQNKLWAGMAKETAHQIGTPLSSLIGWLEIMKADNVDETIVSEIEKDIN
                     RLQTITDRFSKIGSEPVLEIKDIVQETKETFDYLQSRFSKQIEFSFKAPKNPLLVSFN
                     PALHSWTIENLVKNAIDAMKGRGKLALVIKEDGNFVKIKVSDTGKGIPKKEFKKVFEP
                     GFTTKKRGWGLGLSLTKRIVEDYHKGKIKVSESEVGKGTSMQISFKKVEKKS"
     gene            complement(16507..16896)
                     /locus_tag="PK218_05015"
     CDS             complement(16507..16896)
                     /locus_tag="PK218_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPW97900.1"
                     /translation="MASIFTKIVNGEIPCYKIAEDDKFLAFLDVNPNAKGHTLCIPKE
                     EINKIFDMNEDDYLELMKFSRKVAKALEKSVECKRVGVAVVGLEVPHVHVHLIPLQEM
                     DDMRFQRKVSLEKEEFESLSKTISSNL"
     gene            complement(16900..17376)
                     /gene="greA"
                     /locus_tag="PK218_05020"
     CDS             complement(16900..17376)
                     /gene="greA"
                     /locus_tag="PK218_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPW97901.1"
                     /translation="MSTVSYYTAEGLKKLKEELEQLKSIERPKASQAIAEARDKGDLS
                     ENAEYDAAKEAQGLLEMRISKLEEVYSNARIIDESTLDTSKALVLSTVKIKNQTNGME
                     MKYTLVAESEADLKTGKISVTSPIGKGLLGKSVGEIAEITVPNGTLKFEVLEISRD"
     gene            17541..17957
                     /locus_tag="PK218_05025"
     CDS             17541..17957
                     /locus_tag="PK218_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97902.1"
                     /translation="MKKIIVLLAIMTAFLSCDSGATIVNNPNLPNYAVNEALTLSLPQ
                     YSQLQFVSNPIIISAGISGIVVMNTGTSYTAFDLACPNMQFGTCPSAMTLVGLNAYCS
                     CDESTYSLFTGLSQGQQYPMKQYRAELSGGVLYITN"
     gene            complement(17991..20240)
                     /locus_tag="PK218_05030"
     CDS             complement(17991..20240)
                     /locus_tag="PK218_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW97903.1"
                     /translation="MEILFKKSAKQKAGSEKMEVGRKSIIYYLFSIFFPSKSILYSLF
                     SIFFTLTSFAQEKVQDSTKVNQLEEVTIAAVRAKGKDPITFSNLSKEEIAPRNLGQDI
                     PVLLNYLPSVVTTTDAGNGVGYTYMRVRGADGSRINVTLNGIPFNDSESQGTFFVNLP
                     DFASSLESLQLQRGVGTSTNGAGAFGASLNMQTKSYQEKAFAEVANSVGSFGTRKHTL
                     AFGTGLHNNFEINARLSNIASDGYIDRASSNLFGYFFNANYVTQTSLIKLIAFGGKEK
                     TYQAWNGIEDPEKLKDDRTYNSAGMYTDEFGNEKFYDNETDNYWQNHFQLLWTEKWSE
                     KWVSNAALHYTLGKGYFEQYKEDENLSDYNLASFQGNDVSDLVRKRWLDNDFFGATFS
                     LNYKTKDTDVLFGGAANRYLGKHFGEVVWTQYYVPNNNRYYNDIGNKDDVNVYAKASH
                     QIGKVNVFADMQYRMVFYNATSTKFDAVDDTFRFFNPKAGLNFDLNAKNSFYGYVGIA
                     NKEPRRDDYENGSVKPERLIDYELGWKFNSKKVKVSANAFYMSYNDQLVLTGALNDVG
                     SPIFTNSGKSYRVGLEVESVIAITDKLILNPNITLSQNKNQDFYFERDGVLANLGNTS
                     IAYSPDVIVGNRITFLPVKDFQISLLSKYVGEQFMGNIDSNYSKLDAYFVNDLNVSYD
                     WKINKGLKSVTFSALVNNIFDLEYESNGYFYTYDDDWSTPGSISTVEGKGLYPQAGIN
                     FLAGVALKF"
BASE COUNT         6867 a         3528 c         3285 g         6719 t
ORIGIN      
        1 aaacagagct atcttaaaac cagaaggatt attaacaaga gatccaagaa tggtagagcg
       61 taaaaaattc ggtcagaaaa aagcaagaaa gaaattccaa ttctcgaaac gttaatattt
      121 cgaattggtt tggatttttt ttcagacaca tttttacaat taaaattgaa tttaaaacaa
      181 agttgtcgat attccttgta aaaagggaat tagtttagca tctaaatttt caagacaaac
      241 gcaagtgact acttgataat tgctataaca cggaacgtaa actattacac aatggcaaac
      301 aaaattgaag ttaaagactt attagaagca ggtgttcatt ttggacacat gactcgcaag
      361 tgggatccaa acatggctcc atacatctat atggaaagaa atggtattca tatcattaat
      421 ctatataaaa ctgctgcaaa aattgaagaa gctaatgaag ccttgaaaaa aattgctgca
      481 tctggtagaa aagtactttt cgttgctacc aaaaaacaag caaaagacat cgtggccgaa
      541 aaggctgcag ctgcaaacat gccttacatc actgaaagat ggccaggcgg aatgcttacc
      601 aatttcgtta ctatcagaaa agctgttaaa aaaatggcaa tgattgataa aatgaaaaaa
      661 gatggtacat tcatgactct ttctaaaaaa gaaagattgc aagtagatcg tcttcgtgca
      721 aaattagaaa agaacttagg ttctatcgct gatatgtcaa gattacccgc tgcattattt
      781 gtagtagata ttaaagctga acacatcgca gtaaaagaag cacaaaaatt aaacattcca
      841 gttttcgcaa tggttgatac taactctgat cctcgtgaag tagattacgt tataccttcc
      901 aatgatgatg cttcaaaatc aattgataaa attttatctt tagtaacaac tgcaattata
      961 gatggtttgg ctaatagaac atctgacaaa gatgctgaag ttgaaactac tgaagttgaa
     1021 gctactactg aagttgaaac tccagttgct actacagaaa ctagtactga ggaataaatc
     1081 caataaaatt tcaatactga aatcccaaat tccaaacaat catttaacta atgatttcat
     1141 aaagttgggg tttgggattt ttgaattgaa atttcttttt tgaaaacatt tttaatctta
     1201 aaaaataaaa caaaatggca acaattactg ctgcagacgt aaataaatta agaacaatca
     1261 caggtgcagg aatgatggac tgcaaaaaag cattagtaga agctgatggc gattttgatt
     1321 tagcaatcga aaaccttaga aaaaaaggtc aaaaagttgc agctaatcgt tcagatagag
     1381 aatctactga aggtgctgct attgctgtag ttaatgcgga caaaacatct ggagttgcaa
     1441 taacattaaa ctgtgaaact gatttcgttg gaaaaaacga aggtttcgta aaattggcta
     1501 cagaattggc taatcaagca ttaaactatg atactaaaga agcattttta gcttctgact
     1561 ttaatggtat cacggttgct gaaaaattaa tcgaacaaac tggtgttatc ggagaaaaaa
     1621 ttgaaatcgc ttctttcgaa agattaaacg gaacttttgt aggttcttat atccatgctg
     1681 gaaacaagat tgctactttg gtttctcttt ctgctaacgt tgctggagcc gaagaagctt
     1741 caagaaacgt agcaatgcaa gcggctgcaa tgtctccaat tgcgcttaac gaagatggtg
     1801 ttgatgctgc gattattgaa aaagaaatcg aaattgcaaa agaccaattg cgtgctgaag
     1861 gaaagccaga gactatgtta gacaatattg ctaaaggaaa attaaaccgt ttctttaaag
     1921 acaatacttt ggtaaaccaa gattatatta aagacagttc aatgagcgtt gctgcttatg
     1981 taaaaactat tgacagcggt ttaaccgtta ctggtttttc tagagttgct cttggataat
     2041 ttttaattcc aaattaaaaa atacttatac ctcatttctc tattgaaatg gggtttttta
     2101 cattttatac tattgtagcg ttgcaaaaat acattccttt atcaaagtaa aattttcttt
     2161 aacccttcag ccacacttat ccaaaggaaa ttataaaagg atttctctgg atttctatca
     2221 acttcgatgg ttgtttctgt aatcttatct ttggtgtcat ctttcacaaa aatattagca
     2281 ataaaactca atactttatt tttctttttg cgcgcatctt tttgatagac tgtaaacttt
     2341 aaatcatcat attgcacggc aaattgacct gaacttttca aatcattacc cgaaaaatta
     2401 aatcgaactt catccaaaat accttttgtg gttacattca tatagggttt ggtaaaggca
     2461 ttaatttgct gggcttcaaa attgcctaat tttccttgta tcttaaaacc atctcttttg
     2521 tcgaggacat taaaactcca attgaccgta aacggagagt ttttcataaa tttgcaatga
     2581 atggcaatct ttacatcgga gagtttttct tttttaaaac cactgtttaa agaggtagcc
     2641 gttaaattaa agtcattaaa ccgcaagacg ccagctccta tttcagaatt tttttcttct
     2701 tcataaacca atttagaatt ttgaactttt agggtgtcaa cttgtaaatc aaatttcaaa
     2761 tctcggagca atttgttata caagtgcttt ttgctcaagt catcggtggg ttctttggag
     2821 cgatatatgt tggcgttgac attatctaac gttatcaaat tacaatgaaa gaagaaatcg
     2881 tctcctctaa atccccagtt actgtttttt aatgaaattg atttgcaaag cagtgtataa
     2941 atatcctttt cattagtcaa tttagacaca aactctcttc ttgaaaaagc aggaatcatt
     3001 tcaaaattat ttatctgtgt ggtgtttttt gtaatgttaa ctttattggt ttttagattg
     3061 taaaaggaat tgggatgata aaataaacta tcgcaggaaa ttgtataatt ttcatattct
     3121 aaaggaattt ttttatttaa aatttcttca gttattttaa ttccttcaag gttgaaatta
     3181 atattattga cacttaaaat gggcactgct tcatttttag aaagcacctt tagattgcca
     3241 ttgcttagca aaatattaga aactttaata atcttttcaa aaggtgctac tactgaatta
     3301 cgaactgaat aatggttatt attatcataa acaataatat taggcgattt tattccaata
     3361 gtagatgctt ttattttatt gctaaaaatc aaattccaaa catttatatc tttaatctct
     3421 atggtttgta tattggcgta aattcctttc tttgaaatgt tatcttttaa actagatttg
     3481 ggaactacaa caatattttg agcaaaaatg catcttctta acaatgaaac ttctagtttt
     3541 ttataggtaa taaaataaga agtatcattt ttttcattga taatagtggg caattgatgt
     3601 tttatccatc cattcaataa aatattgagt acaaacacaa caagcaatat tgttagaaca
     3661 cctagcaaca tctttttata tactttgaca gtcatatttt ttgagtttgt tgtttataaa
     3721 tttaagtaat ttaataaagt tagcttcatt ctttatattt attttaatac taaatatttt
     3781 ttagtttaaa aaagtatgca taaaagttac ttttgtagca gtatttcaag aaatagttta
     3841 gagaatttac ggtttaatga tttctttttc tatttttgtt tttcagtata tctcttaatt
     3901 gattgtactt acaaatcgca taaaataatt ttgcattact atgaaatatt ttaaactaac
     3961 gatgttactg ttatctgttc taactttaac aaattgtagc tcagatccag aaagttatag
     4021 caacccgcaa ggaaatccac cacctggagc tactttacca acacttgcta ctgccgatgt
     4081 aacaaacatc agttttacta cagccgtttc gggtggcatt ataacttcag atggtggttc
     4141 accaattata gccaaaggaa ttgtttggag tttggctcac aacccaacaa cacttctagg
     4201 cactaaaacc aatgaaggtg aaggaagtga tgtttatact agtaacatga caggcttgga
     4261 gtcgaataaa acatactatg ttagagctta tgctcgaaat ggaaaaggcg tttcttatgg
     4321 tcaagaaatt gagtttaaca cacttgttga tgtaaattct ttacctgcag taacaacagc
     4381 acaagcaact aacgtaacga caaactctgt tactataggc ggaaacgtaa ctaatagtgg
     4441 tatttctcca gttactgccc gtggagtagt ttggacatta tcgcccaccg ttccaccaac
     4501 agttttagta aatttaggaa aaacaatcaa tggtgttggt ttaggaactt ttgttgataa
     4561 tttaagcaat ttaacaccaa acattaccta ttatgttaga gcttatgcta caagtttata
     4621 tggaacaaac tatggcgaag tagtttcttt tactactagt cctttattgt acacgattgg
     4681 aaccagtgtt acagacataa acggtaatgt atatggttcg gtaaaactga acaaccaaca
     4741 atgggcaact aaaaacttga gcgttacaaa atatagaaat ggagatgtta ttccgcaagt
     4801 gcaagatgct acacaatggg cagcattgac tactggtgct tggtgttatt attcatatca
     4861 atcagccaac ggtacagttt atggaaaatt atataattgg tatgccgtta acgacgcaag
     4921 aggattagct ccaacaggat ggcacattcc aactaatgaa gagctgatat cgcttattga
     4981 ttttcttggt ggcacagaag cagcaggcgg attgttgaag gaaactggca caacacattg
     5041 ggaagctcca aacactggag caataaactc tagtggtatt aaagcactac ctggtggata
     5101 ttgtttggca gatggtacat ttagtaattt aggaacaaaa ggctattggt ggattgctga
     5161 cagttacaat ccttcgagtg catggtgtgc tggtttatat aatgatacta aaacaattac
     5221 tcgcgctcca attgacaaaa gacaaggatt ttcagtaaga attgtaaaaa attaatattt
     5281 cataataaat cattccaaaa acctcatttc ttaattgaaa tggggttttt ctatatattt
     5341 gtgaagaacc taatacaaaa tcacttgaga aattatatac tttcgttact acttgtttgt
     5401 ctaactactt cagcacaaga aaaaacttta ttctgggaaa tatctggaaa cggattaact
     5461 aagaaatcat atttgtatgg cacaatgcat gttagcgata aggtttccta tcatttatcg
     5521 gatgcttttt ttaagaatct actaaatgca gacatggttg ccaatgaaag cgatccagaa
     5581 acttggaatg atttaaccga tgttgttaaa gaaccaaact ttgatgccaa agctaatttt
     5641 tatactgagt tctataaaag tgttgtaaat aaaaatgatg ttaaagcagt atttaacatc
     5701 acaaataatt tttttaacaa ccttttatct tccattggtg gagaaacctc ggactatcaa
     5761 gaaaacacgg ttttggatat gtttatatat caaaccggaa ggaaatataa caagaagaat
     5821 gtaggactag aagatgccaa aggttcattg attcctatat taaaaataaa agaagaagat
     5881 gctaaaccca aagatgaaaa ccttcaattg ttggttaagt tgatgaaaaa ccgtccgttt
     5941 aacgaagtgc taaaacaata ttatagagaa aaagacatcg taatgcttga ttctatttac
     6001 aagctaatga tgtctaaaaa agcccacgat gttttgatta ctattcgaaa cgagaacatg
     6061 actaaaagca ttgactcctt ggtcaaaaaa gggagtttgt tttcggcagt tggtgcggca
     6121 catttggctg gtaacgaagg aataattgct ttgcttcaaa aaagaggcta taccgtgaaa
     6181 ccaattgtag atgttttttc ggaaagcgga aacaaacaaa agaaaaacat tgaagactat
     6241 tttcccaatc caggctttgt aacgacaagc accagtgacg gaatgcttac gatgcctttg
     6301 accaaaaatg tttttgaaga atttgcaact attggcgcgc ccgattttac aaatggcggt
     6361 gttatcaaca tcaggagagt tcctttaaat tattatttaa acaaaaaaga aaatctatac
     6421 aatataaaag cgttagacag tttgtttttt gaaaacatcc caggaactat tttggacaaa
     6481 aaaatgtttg aagtcgataa catttcgggg tatgatgtga aaaacataac caaaaccggt
     6541 agcaaccaac gttacaaatt ttatgtaaca ccattagaaa ttataggagt ttctatgact
     6601 ggtgttggaa attatgtgcg ccaatttgaa aatgaaatct ttgacaacat aaaacttaaa
     6661 tcgagcaaag aaacatggga aaccatgatg ccaaacaaag gcggcttttc agttgaatta
     6721 ccttctttct atagtgctta tggaaataac aaaagttatg aagaccttga aattcaggct
     6781 tatgacccag caactaaaag ttattacttc ttgactgaaa aaacgaataa tgacacggat
     6841 tttttagaaa actctgaatt tgaacaacat caaattcagt atgaattcta tttgcaacac
     6901 gatgcagcca tagaaaactc caactatgat aaagtatcaa agtcattaga aacatcatca
     6961 aagattgggg ataaaaatat ccgattgaaa actatcatca atggggataa atactattta
     7021 ttgggcacaa taaacgcttc tgaaagtgat gccaaccgtt tcttttcttc atttaaaaca
     7081 acatcgttta tttataaaaa tgaaacttca gtttttaccg attccataaa caaatatcgc
     7141 attgaaattc cgaaaaaact gaacgataaa ttatttttaa atctaggttt caaagaaaaa
     7201 gataccaaaa atcaatttga agagaaaacg aaatatgctt cttatcgctc ggaaacaaga
     7261 aagaacgtaa cgcttcttta taataaattc tcaaaatacg aaagcattgc tactattgat
     7321 tcgttgcgta ttaattttag agaatacttt ttaaaagaag atagcaattt taaaaaagat
     7381 acttatgacg attatgatta cgaccatgtt tcttcacttt taagtgtaac atataaacac
     7441 aaaggcatta caccttcaaa atgggatgat tatatccaaa aggaagaaga gaaatatgaa
     7501 atgctttcgg aaacttttga ttatgataaa gaaaaaaaca tctatgtact aaacaacata
     7561 gtttctagac aaaacacatt gcaagccatt aaatacaaaa cggtttttat caatgattct
     7621 tattatatgt tgagcacact aatcaacaga aactataaaa atgatgatgc gtttattgaa
     7681 aaaacattta attccattga accttatggc gaaaacttga accacaacta ctctattttt
     7741 gacgataaaa taaatttgtt catcaatgat gccaatagtg aaagtgatac tatccgctat
     7801 tctgctatga attcggtcta tgatttagat ataaccaaaa aagatttgcc tgcaattaaa
     7861 acattcatcg aaacgtttac ttttaaagac agtgaaactt cggcaattca atctctgatg
     7921 aaaaaaattg gtttaattca agaagaaagt gtcattcctt ttttagacaa tttataccga
     7981 aaagaagacg ttaaaacaac cattcaactt ggcgttattc aagctttgat taatcaaaaa
     8041 actaaaaaaa gttaccaaaa aatcatcgaa cttttagaat atgatttacc catttcagat
     8101 aatgaatatg atataaccaa tctttttgaa aattttgaag acgatttaga acatagcaaa
     8161 gaattgtttc caaagatatt ccaattttat agtgtaaaag aatataatga acccataatt
     8221 tctttctgca acttattgtt agatcaaaat ttagtggaag gaaacaaaat taaatcgttt
     8281 aagaaaatga ttttaaccaa cgccaaatta gaatacaaac gtgttgccag ttggaaagaa
     8341 aaaaataaac ccgatgaaga aactattgct agtgacgatg ttgttgaaga ttatgactat
     8401 aatgctaaca gtgccaatgt aaattcgttg aaaaattata ttggtttgtt gtatgagttc
     8461 aaaaatgaca cagctataaa tgactatttt aagaaggtaa aaaaattaaa cttgcccgaa
     8521 attgacttag agtttacacg tttaggaatt gtaaacaatc gcattagtaa tgaagaaatt
     8581 caagcggcat taaacaatga ggaaaatcgt tttgtagtta tcaatctgtt attaagtaag
     8641 aacaaagatg ttgctataaa actatcggat gatgaaattg caaaatcagc tttattgaat
     8701 ttgaaaggat tgacagtaaa agacactatt aattatattg acaaaaaaat agtcgaacaa
     8761 gcaggcaaac aaacagtata ttatttctat aaagccgaaa aaaaatcata tagctacgat
     8821 attgctaaaa aaataatcta tacgatggct tttgttttag acaataacaa aatcaatccg
     8881 ttagctttta aaatgtttcc agatgaattt ttagaagatg atgatgaaat gaataaaaca
     8941 ttagatttaa tcatcaaaaa atcaatgaat gaagaccaca aacgagcaag ttttgagaaa
     9001 gtagatccta ataaaaacag atacttctac aatgattatt gattataaat ttgtctaaac
     9061 ttttgggtgc agtctatcgt ctgaatttgg cttttttatt atttcaattt aatttatttt
     9121 ttttcaattg gaaaatttct agcacgcatc aaagcatctg atgtagctgc ttttcctctg
     9181 aattttttat aggcaatctc agaatcaatt gtatttccaa tactgaaaat atcatccaaa
     9241 agtcgttttg caacaacttt gttatacaat ccatctttgg tttcggtaaa tgcttcagta
     9301 gcgtcagcac taataacatc tgcccaaagg tatccataat aaccagctgc atattcatca
     9361 ccagaaaaaa tatgcccaaa ctgtggaatt ctatgacgca tcacaatttc cgatggcata
     9421 tctaatgatt ttaaaacctc tttttcatat ttagaaggat caatgtctgg attttccaac
     9481 aagtgtaatt tcatatccac cagagcactc gaaatggttt ctactgttga aaagccttcg
     9541 ttaaaagtag aagctttttc aattcgttct actaaagcca ttggcattgg ttttccggtt
     9601 ttatagtgca aagcaaattg atttaaaact tgtggtgttg ccaaccaacg ttctaaaacc
     9661 tgcgatggaa attctacata atctctaggt gtattggttc cagatagcga tggataggtt
     9721 acattagaac acaatccgtg aagagcatgt ccaaattcat gaaataaagt gctggcatct
     9781 tcccaagaaa ttaggatagg ttcgttttct tttcctttaa taaaattaca gttgtttgtc
     9841 actatcgtaa tcacatcgcc gttaactcta ctttgctctc ttaatgcatt catccatgcg
     9901 ccagatcgtt ttcctgttcg tgcataagga tcaaaatacc ataatccaac cactttgccc
     9961 gaagttttat tggttacttc aaaaactctt acatctggat gataaaccgg taaagtaaag
    10021 atttgtttga aatttaattt gaaaatttct cctgcaaccc aaaacattcc ttctctcaat
    10081 ttttctagtt gcaaataagg tttaacatca ttttcatcta aatcatattt ttctttacgc
    10141 actttttcag cataataacg ataatcccaa ggttctattt taaaatttcc gccttcagct
    10201 tctactatct tttgcatttc agcaacatct ttatgcactt tttcaacagc tggtttccaa
    10261 acagattcca tcaatgcaat tgttttttca ggtttttgag ccatggtatt tgacaaactc
    10321 caatcggcaa aagttttaaa tcctaataat tttgcctttt cagctcttaa ttttagaatt
    10381 tgaaccaatg ttgttttgtt gtcgttttga ttatctccgt caccacgatt tacaaataat
    10441 tcccaaacca tttttcgtaa cggacgaaaa cttgaatacg tcaagaacgg ttccacactc
    10501 gaacgagtat tagcaactgt tcctaaatca tttcttccta attcttttga ttttgttaga
    10561 tacccattta tcaaatcggc tggcaaacct gctaactcat ttttttcagt aaaactaatg
    10621 ctgttttcat tttcatcatt caataaattt tgactaaact tagtaaacaa tcctgctaat
    10681 tcttgattaa tttgagctat acgtactttg ctttttttgg ttgcttttgc tccttcacga
    10741 acaaaattgg aataatacaa ccaaaccaat cgttgttgtt ctggggttaa cttatttttt
    10801 tcaggtgaat tataaacagt ttcaatacgc tgaaaaagtt tttcgttttg ataaatttta
    10861 tttgaaattt ctgaaaactt gggcgacatt tcagtttcta tcggagtaaa ttcgggtgta
    10921 ttcatgttgg aactataaat tccaaatact gcataaatct gacttaattt tttacccgct
    10981 ttttccattg ctacaatggt attttcaaaa gtaggtggtt ttttattgtt ggcaattgtt
    11041 gtaatctcgc tcaatttttc attgatagca ttttcaatcg caggttttaa atccttcaat
    11101 ttgtattggt caaacgcagg aattccacca taaggtcctg accaaaagtt taaaaacgga
    11161 ttgtcatttt tttgagcatt cattgaaatc atagggaaaa tcaatatgag attgaacatt
    11221 atttttttca taattatttt gaatttattc aaatataaaa aaattgattg agattcattc
    11281 taaaaagttg gtgcttttat ttacatttga taaaatcatt gattttaaaa aaaactcatt
    11341 atttatgcaa aataataatc cacttcacgg tattacttta caaaaaatag taaccgattt
    11401 agtagctttt tatggatttg acactttatc tgaactcata aaaatcaatt gttttaaaag
    11461 caatccgagt gtaaaatcga gtttgacttt tttacgaaaa actgattggg caagaaaaca
    11521 agtagaagat ttatacatca aaacattacc taaaatcaat aaaaatggaa actaaaaacc
    11581 gttgtgcttg gtgtgagaaa gacgacttat accgagaata ccatgataat gaatggggaa
    11641 aacctgttta tgatgatgat aaacttttcg agtttttagt attagaaacg tttcaagctg
    11701 gtttgagttg gcacatgatt ttaaaaaaaa gagaaaattt cagaaaagcg tttgctaatt
    11761 ttgattacaa cctcatcgca gcttttgatg aatatgaagt acaagaatta cttaaagacg
    11821 tagggattat tagaaataaa ttgaaaataa aagctactat tactaatgcc attgctttca
    11881 aaaaagttca aacagaattt ggtagttttt ctagctacat ttggagtttt gttagcggaa
    11941 aaccaataga taataatccg aaaaccttaa aagatgttcc tgcttctact tcattatcag
    12001 atataattag caaagattta aagaaaaaag gcttcaaatt tgtgggaaca acagtagtgt
    12061 atgcgcacat gcaagcaacc ggaatggtaa acgaccacgt ggaagattgt tggacgagaa
    12121 gttaatttta ccgttttaaa aaataatttt tgatacacat taaaattgta cctttagttt
    12181 aaaattacac catcatgttt cgtcataaag gaagcagaag aactttctct cacaacattc
    12241 gattagcctc aacgctttct tttgtagcag gaattgtaaa cattgcaggc gttttatccg
    12301 ttgcgacatt aacgaccaat gtaacgggac attttgcttt ttttgccgaa gaaatttccc
    12361 tgaaaaatta caccaaagca ctaaatatta ttgtgtacat tttctttttt cttttgggtg
    12421 cttttacttg caatttattg gtggaaatcg tttctagaaa aaaaccacga atggctcatg
    12481 cattaccaat gttcactgaa atcgtaattt taacatcagt cggtttttca acagatttta
    12541 atccgaatta tattgcctat gccttacttt ttgccatggg attacaaaat gctttggtaa
    12601 ccaatatttc acaatcggtt gtaagaacta cccatttaac tgggcttttc accgatttag
    12661 gtatcgaatt gtcgcagttg tttttctata aaaaagaaac cgaaatgaaa cgcttaacac
    12721 gaagcatctt cttacgaatt tcaattatat tatttttctt tatcggtgga gttttgggcg
    12781 gattgctctt taaatcattt caattaaaaa tcttactctt tgcagttgtc atacttgttg
    12841 ttgctctatt tctagacaac gctataattt actacaactt aaccaaaaga aaaataaaaa
    12901 tcatgacttc aaaatcttca taaacgtttc tctagaaatt tcaaatgcgc caagtttttc
    12961 caaatggtcg ttgtgtactt ggcaatctag caacttataa tttttttctt ttaagtgatt
    13021 aatcaaggtt acaaaagcta ttttactcgc attagaaact ttggaaaaca tgctttcgcc
    13081 acaaaataca tgacccaaat caactccata caaaccacca accaactcat cattttgcca
    13141 aacctcaacc gattgtgcaa aacccatttc gtgaagtttc acataagctt caattaaatc
    13201 attcgtaatc caagtaccat cttggtcttt gcgttctatt tgttggcaat tggtaatcac
    13261 tgctctaaaa tcatgattaa acgtaacttt aaattttttt tgattcaata aatttcgagt
    13321 gcttttgcaa attttataca attgtggcgc aacaaccatt ctttcggatg gcgaccacca
    13381 aaggattggc tcttctgcat taaaccaagg aaaaatccca ttgcgatatg ccaaaagcaa
    13441 tcgctcagta gataaatcgc caccaatagc caaaatacct tcgtatgaag cttcttcaac
    13501 tggtggaaaa taaagttttt tggttacgta atacattttc aaaaattcca aaaaataaat
    13561 tccaaattcc aaaactgata caatttggaa tttggaattt taaactattg ggatttactt
    13621 tttaattttt tattaaaaag gtaaatcgtc gtgttcttct tcgttaaagt tggttgctgg
    13681 agcaaaagct tctgccggtg gcattggtgg cattcctgct gctggtgctt ctgcttgaag
    13741 tttttcaact ctccaacctt gaatatcgtt gaaatatttt gtttcgcctt gtgggttaac
    13801 ccattctctt cctctaaggt tgattgaaac tttcacggca tcaccaacgg cataattgtt
    13861 taataaatca catttatctt gcacaaaatt gatgctgatg tattgtggat attgttcttc
    13921 agtggtaaca accaattctc tttttttaaa ggtagctgta acttgttgtt ctgctcctac
    13981 tactctaatt tttcctgtaa cttccataat tataattttg ctaaaagtac tttccatgcc
    14041 gaaagcacat cattattatt caaatattct ttagctttca agtgaatttt atcggtgtca
    14101 tccgaactca aaacaccttt tacagcggta ttattttgta caaagatagt aatttcttct
    14161 tcgctaggca atgcttctac atttcctaat ttacccaaat catttccatc aaaaacagtg
    14221 ctttctctga taaattcagg aatactatct actcctattc ctagtgttga caacggtttt
    14281 tcgacttcaa acaatccttg attggaacgc gaataccaat tgccgccaag acgtgctact
    14341 aggtcgattt tgctttgatc aatcatacct ttatcgtcta aaatagcttc attaatgtga
    14401 atctttacta cttcacaaat aatcagattc ccagcgcctc cttgatctcc gagtgctatg
    14461 atttcattga ctttacattc caattgcacc ggactttcgg ctacgcgata gggctttatc
    14521 atatccgaag gcaacatggt aagtcctgat tttaaaaatt cgttcacacc atctaaatat
    14581 tctgtgcttg ataatgatgc ttgttgtaca atatcataat ttaccacgtt aatcaccact
    14641 tgtttcgtag cgatacagtt ctctaacgta tgttttgtgg tattatttct aaccctacgt
    14701 gcaggcgaaa acaccaaaat tggcggatta gagctaaaca cgttgaaaaa actaaacggt
    14761 gataaattag gtcttccttt ttcgtcgata gtacttgcaa atgcaattgg tcgtggtgca
    14821 acggcactct gtagatagcc ttgcagctgc gcaggcgaaa gagtatgtgg ctcaaaactt
    14881 aacatagaaa tgtattttga acaaaaataa ctatttaaaa tagaatttga agttaagaat
    14941 taaagtttca ttggtatatg tataaaattc tcctgcggta aagaaagatt tcaatattta
    15001 aaaggctttg tagccttcaa aaggtcttcc gtgtccttca aaaggacttc cgtgtctttc
    15061 aatgatgctt ccaattgctt caaaaatata gccgttgctt tcaaaaatgc ttccgtggtt
    15121 gacaaattgt tttccgttgc tttcttcttg tcttttcatg ccttcaaaat atctttctaa
    15181 ggcaaaaaaa ggttttccgt tgggttcaat aatgctttca gagctttttt tgattatttg
    15241 ttatttttcc taattgattt tatcatcaaa tcagcgattt ttataggata atcatttttt
    15301 tagtacattt aaaccaaatt atttcagatg caattttcgg aaagaagaaa cagtactcgt
    15361 tggattatca tcatcacgtc gtttgtgatt gttacactta tactttggaa cacgtattct
    15421 ttctttcaaa tatttaaaaa cgaagaacgc atcaaaatgg aactttgggg cgaaagcatg
    15481 aaaacgttat taaacgctga tcctgaaact actgaagtag aattaccaag acaaataatg
    15541 agcaacaaca ctaccattcc aatcgttttg gttgaaaatg gagtaatcat caatcaaacc
    15601 aatattgatg aagaaattac gaagaacaaa actaaattaa atgctttttt agaaaaatta
    15661 aagaaagaaa acgagccaat caaaattgaa tacgacaaaa ataaatacca atatttatac
    15721 tacggcaact cttctttgtt gaacaaatta aaatattatc ctgtggctct gttgttgatt
    15781 attttcttgt ttggagcagt agtttacaac ttctaccgaa gcacaaaaat ggcaacccaa
    15841 aacaaacttt gggctggaat ggcaaaggaa acagcacatc aaattggcac acctttgtct
    15901 tctttaattg gttggctcga aatcatgaaa gccgacaatg tggatgaaac cattgtatcc
    15961 gaaattgaaa aagacatcaa ccgattacaa accattaccg ataggttttc taaaattggc
    16021 tccgaacctg ttttagaaat caaagacatt gtgcaagaaa ctaaagaaac ttttgattac
    16081 ctacagtctc gtttttcaaa acagattgaa ttttcgttta aagcaccaaa aaatccgctt
    16141 ctcgtttctt tcaatcctgc tttgcacagt tggacaattg aaaatttggt aaaaaatgct
    16201 attgatgcta tgaaaggcag aggaaaacta gctttagtca ttaaagaaga tggcaacttt
    16261 gtaaaaataa aagtctccga tactggaaaa ggaattccca aaaaggaatt taagaaagtt
    16321 tttgaacctg gttttaccac caaaaaacgt ggttggggtt taggattatc tttaaccaaa
    16381 agaattgtag aagattatca caaaggaaag attaaagttt ccgaatcgga agttggcaaa
    16441 ggaacatcga tgcagatttc atttaaaaaa gtcgaaaaaa aatcctgatt gctcaggatt
    16501 tttatattat agattagaac ttatcgtctt tgacagactt tcaaactctt ctttctctaa
    16561 actcactttt cgttgaaaac gcatatcatc catttcttgt aacggaatta aatgcacatg
    16621 tacatgtggc acttcaagac caacaacagc aacaccaaca cgcttacatt cgactgattt
    16681 ttctaatgct tttgcaactt ttctagaaaa cttcatcaac tcaagatagt cgtcttcgtt
    16741 catgtcaaaa atcttattga tttcttcttt cggaatacac aaggtatgtc cttttgcatt
    16801 cggatttaca tccaaaaagg ccaaaaactt atcgtcttct gctattttgt aacaaggaat
    16861 ttctccgttt actattttgg taaagatact tgccatgctt tagtcccttg atatttctaa
    16921 aacttcaaat tttaaagtcc cgtttggcac tgttatttcg gcgatttctc caaccgattt
    16981 ccctaataaa ccttttccaa ttggtgaggt aaccgaaatt ttaccagttt ttaaatcggc
    17041 ttcactttcg gcaacaagcg tatatttcat ctccatgccg ttggtttggt ttttaatttt
    17101 aaccgtagat aacactaacg cttttgaagt gtcgagagtt gactcatcaa ttattctagc
    17161 attagaatat acttcttcca atttagaaat acgcatttcc aaaagtcctt gagcttcttt
    17221 ggcagcatca tattctgcat tttcagacaa atcgccttta tctcttgctt cggcaatggc
    17281 ctgtgaggct tttggtcgct caatgctttt taattgttct aactcttctt ttagtttttt
    17341 taatccttct gcggtataat acgatactgt actcataatt tcactctttt atataaatag
    17401 aaaaaatccc atcgcaacgg gatttctttt tgcaaagata ctctttttaa tttatttcaa
    17461 ttttttttat cgccgaatag cgtttgccaa agttaaataa tttatttttg tttaaacaat
    17521 atttagaatt aaaaaacact atgaaaaaaa taatcgtctt attggcaatt atgaccgctt
    17581 ttttaagctg tgattcggga gcaactatag taaacaatcc taatcttcca aattatgcag
    17641 taaatgaagc cttgacttta agtttaccac aatattcgca attacaattt gtaagcaacc
    17701 ctataattat cagtgccgga atatcgggaa tagtagtcat gaacaccgga acgagctata
    17761 ccgcttttga tttagcctgt cctaatatgc aatttggcac gtgcccgtct gccatgacat
    17821 tggtgggttt aaacgcctat tgtagttgtg atgaatctac ttatagcttg tttacaggct
    17881 taagtcaagg acaacaatat ccaatgaaac agtatagagc cgagcttagt ggcggagttt
    17941 tatacattac caattaaaaa aagtccccgc caaggcgggg actttttaat ttaaaacttc
    18001 aatgcaactc ctgccaagaa attgattccc gcttgcggat acaatccttt tccttccaca
    18061 gtacttatac ttcctggcgt gctccaatca tcatcataag tataaaaata gccgtttgac
    18121 tcgtattcta aatcaaagat attgtttact aatgccgaaa acgttaccga tttcaatcct
    18181 ttgttaatct tccaatcata cgaaacattc aaatcgttta caaaataagc gtccaacttt
    18241 gagtagtttg aatcgatatt tcccataaat tgctcaccaa catatttaga cagcaacgat
    18301 atttgaaaat ctttcacagg caaaaacgta atgcggttgc ccacaattac atcgggtgaa
    18361 taagctatac tggtattgcc taagtttgcc aaaacaccat cgcgctcaaa atagaagtct
    18421 tggtttttgt tttgactcaa cgtaatattt ggattcaaaa ttaatttatc tgtaatcgca
    18481 atcaccgact cgacctcaag accaacacgg taactttttc cgctattggt gaatattggt
    18541 gaaccaacat cgttcaacgc gcccgttaaa accaattggt cgttgtaact catataaaaa
    18601 gcattcgccg aaaccttcac cttcttcgag ttgaacttcc aacccaattc ataatctatc
    18661 aaacgctctg gtttcacact tccgttttca taatcatcgc ggcgtggctc tttgttggca
    18721 attccaacat agccataaaa cgagttttta gcattcaaat caaaatttaa acccgctttt
    18781 ggattaaaaa atcggaaggt atcatcaacg gcatcaaact tagtactcgt ggcgttataa
    18841 aaaaccattc ggtattgcat gtcggcaaat acgttgactt tcccaatttg atgcgacgct
    18901 ttggcataca catttacatc gtctttatta ccaatgtcat tataataacg gttgttattg
    18961 ggcacataat attgtgtcca aactacttct ccaaaatgtt ttcctaaata acggttggct
    19021 gctccgccaa aaagcacatc ggtgtctttt gttttataat ttaaagagaa ggttgcccca
    19081 aagaaatcat tgtctaacca acgttttcga accaaatccg aaacatcgtt tccttgaaaa
    19141 ctagcaagat tatagtcaga caagttttcg tcttctttgt attgctcaaa ataaccttta
    19201 cccaaagtgt aatgcaacgc cgcattggac acccatttct ccgaccattt ttcggtccaa
    19261 agcaactgaa aatgattttg ccaatagtta tcggtttcgt tgtcgtagaa tttctcattc
    19321 ccaaactcgt ctgtgtacat tcccgcagaa ttataggttc tatcgtcttt caatttttct
    19381 gggtcttcaa ttccgttcca tgcctgatag gttttctctt ttccgccaaa ggcaatcagt
    19441 tttatcaacg acgtttgggt aacataattg gcattaaaaa aatagccaaa caagttagac
    19501 gatgctctgt ctatatagcc atcggaagca atgtttgaaa gtcgcgcatt gatttcaaaa
    19561 ttgttgtgca acccagtgcc aaatgccaat gtgtgtttgc gtgtgccaaa acttcctacc
    19621 gagttggcta cttcggcaaa tgctttttct tgatacgatt tggtttgcat gtttaaactt
    19681 gcgccaaatg cacccgcacc gttagtcgat gttcccacgc cgcgttgcaa ctgaaggctt
    19741 tctaaggaag aagcaaaatc gggcaggttg acaaaaaacg taccttggct ttcgctatcg
    19801 ttaaacggaa tgccgtttag cgtcacgtta atccttgacc catcggcacc acgaactctc
    19861 atatacgtat aacccactcc gtttccggca tcggtagtag taacgaccga cggcaaataa
    19921 ttgagcaaaa caggaatatc ttgccccaag ttgcgaggcg caatttcttc ttttgaaaga
    19981 ttagagaacg taattgggtc ttttcctttg gctcttaccg cagcaatagt tacttcttct
    20041 agttggttga ctttggtgct gtcttgcact ttttcttggg caaaagaagt tagagtaaag
    20101 aaaatagaga atagagaata gagaatagat tttgaaggaa agaaaataga gaatagataa
    20161 tagataatag actttcttcc cacttccatc ttctcgcttc ccgctttctg cttcgcgctt
    20221 tttttgaata aaatttccat ccgtaataat tgttacgaat aaaaagaggt aattattctt
    20281 tgttaaaaat aaattaatgt ttgtttcctg tgacaaagtt cagcgtgcac gctagttttg
    20341 tcacttttcc ctaaacagca ttacctgttc taggttcatt gggtataatc tcagctcgt
//
LOCUS       DAOOBH010000030        19984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_3577_length_19984_cov_6.670079, whole genome shotgun sequence.
ACCESSION   DAOOBH010000030 DAOOBH010000000
VERSION     DAOOBH010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 19984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19984
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3577_length_19984_cov_6.670079"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="PK218_05035"
     CDS             complement(<1..1503)
                     /locus_tag="PK218_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="G-D-S-L family lipolytic protein"
                     /protein_id="HPW97904.1"
                     /translation="MIKNIKWLAFVALAFVACNSDDEVIVDPNSTDGTPLTAGSADFS
                     KYVALGDSFAAGYSDNALFMEGQKNSYPNIIASQFALVGGGDFNIPFMADNIGGFSSG
                     GMQSSAYGVRLYFGKKEGSDFDEPLKVDGVSSTDISSMLSGLYGNLGIPGAKCIHLVT
                     PGYGSSFGNPYFARFATSPSTTVLNDAVSQSPTFFSLWIGGNDVLGYALAGGDSTLNP
                     ITPSSGGIGVGFDASYNEIVSQLVTGGRKGVVANLPYITTLPQFTYINPNAVVDPFKY
                     YVDGDENKTSRVVSVGDVATINTINGILGFLDQVLTAYGQGSRINVLSTTTANPVLMK
                     DDELADYGAQITGAALGSGNPQLIALSSYLGATFGKVRQTTTGDLIPLVTSSVIGTDA
                     ALPPGVPADLGKYGITYPLEDRHVLIPSEIVEISNATDAYNITIKNAADANGLAFVDA
                     KAIMQKLSTTGIVANNYTLTSTFVTGAAFSLDGIHPSPRGYALIANEFLKA"
     gene            complement(1521..4427)
                     /locus_tag="PK218_05040"
     CDS             complement(1521..4427)
                     /locus_tag="PK218_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW97905.1"
                     /translation="MKIYLFIISLFFCSISFAQTTITGSVKDGNNEPLPGANVTVSGE
                     STGTITDNEGNFILSTSKKPPFEIKISSIGYGEKMVSISTNNQKVTVVLSDERMLLDE
                     IVVSASRTPERLRESPVTIERFTAADVKKTASPTFYDGLENLKEVQMNTSSMSFKSIN
                     TRGFATVANTRFMQLVDGMDNSSPLLNFVLGNLIGVSDIDVASVELLPGASSALYGAN
                     AFNGILFMKSKNPFTDQGISVYAKYGQTNQKAAGANLYYDYGIRAAHKFNDYFAAKAN
                     FTFMKGTEWWATDYSDKTVEGRDRSHPNYNGMNVYGDEATTNIKSVGQTLVGLGLLPA
                     SALNLLPNENVSRTGYNEVDLTDNVVKNAKVDFSLHFRPGGNENFEIIWQSKFGFGNS
                     VYQGANRYYLNGFTMQQHKLEFTGKNFFLRGYFTTEDGGNSYDMLFTGLNINRAWKDD
                     KTWFGQYAGAYVQSTLAGATPQQAHATARGVADTGRFLPGTAEFQNAFKNVIANPDVL
                     SGSKLVDNSKIYHSDANYNFKDLVKFAEIQVGGSYRRYDLNSFGRIYTDTPQYGGIKY
                     DEYGVYTQVQKKLMDDRLKLTGSVRYDKSDNFDGNYSPRISAVYSAGESKNHVFRGSF
                     QTGFRNPTTQDQYIGFNVGNAILLGSAPDNLTRYSETLPVTTTIGQIYASGDPLNPAT
                     STTINGNNAYYNSYTSASVTAFGAFASANPGNLPGAAALLEKTNVGLVKPETVKAFEL
                     GYRGQYEGFNIDINGYYNIYNNFIGNLTVLAPLYGTAGTTFDFTKGATNPVPYLSADP
                     NQNNPEVQSLQALQSGNYRAFQLYTNTDIEIKSLGFGVGLSKKLPKNFEVSANYNYAQ
                     FDFDQAKDPSFEAGFNTPKHRVKGSISNDNVFKNFGFNVSARWSDEYLWESTFADGMI
                     DAATVIDAQLSYGIPTLKSTLKVGATNLGGKEYRQLLGPGLIGQQYFVSLTINP"
     gene            complement(4709..6556)
                     /gene="glmS"
                     /locus_tag="PK218_05045"
     CDS             complement(4709..6556)
                     /gene="glmS"
                     /locus_tag="PK218_05045"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPW97906.1"
                     /translation="MCGIVGYIGHREAYPIVIKGLKRLEYRGYDSAGIMLFDGNDLKV
                     SKTKGKVSDLEEKSIDIANANATIGMGHTRWATHGVPNDVNSHPHLSNSGKIAIIHNG
                     IIENYEPLKKELINRGYVFKSDTDTEVLVNLIEEVQKKEKLKLGKAVQVALNQVVGAY
                     AICVFDVEKPDEIVVARLGSPLAIGVGKDEYFIASDASPFIEYTSNAIYLEDEEMAVI
                     RRHKPLKIRKIKDDSMVDPYIQELQMNLEQIEKGGYDHFMLKEIYEQPNVIKDTYRGR
                     LHSNQGLIQMSGVEDNLEKFLNAERILIVACGTSWHAGLVAEYVIEEFARIPVEVEYA
                     SEFRYRNPIITAKDIVIAISQSGETADTLAAIKLAKEKGAFVFGVCNVVGSSISRESH
                     AGAYTHAGPEIGVASTKAFTTQITVLAMIALRLAKAKGTLTNSDFFRYLQELEIIPEK
                     VAEALKTNDIAKEIASIYKDAPNCLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAE
                     MKHGPIALIDEKMPVIVIAPKQGHYDKVVSNIQEIKSRSGKIIAIVTKGDTQVKQLAD
                     HVIEIPETSDALSPLITTIPLQLLSYHIAVMRECNVDQPRNLAKSVTVE"
     gene            complement(6569..8278)
                     /locus_tag="PK218_05050"
     CDS             complement(6569..8278)
                     /locus_tag="PK218_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HPW97907.1"
                     /translation="MNFKSYLLRFFSFTIFAMMISCDKDFNEIGTNIVGGDNDHYLFD
                     RYENASIKAYNQKIGAVAMNNLPINPLGIYKNPVFGTTTAGFVSQLEMASGSIGRKFN
                     NTDSTLYQDLPIIDSVILNVPYYSKVIGTVTSEGISTYQLDSIYGEAGSKMRLSVYES
                     NYFLRDLDPTAQFTEQQVFYNDMQEIENQKIPFVLNNLSVFDDKNLDGHENAQFYFDK
                     REHKTTVKNSENVDVPTRSVPSLRLHLDKATFYNKIINAPTSQLASNTIFKNYFRGLY
                     FKVEDLGNSNSMAMLNFANANAKVTVYYTEDLKVTIGSTTTFEKKNKTFDLNFAGNRV
                     SLQSNSNENMDYLSAANATQEASKLYLKGGEGSIAIIDLFGPDADNNGIADEVEQIKA
                     NGWLINEANLTFYIDKTAITGQAIEPNRLMLYDLNNNRVLLDYLTDQSLNSRNPKQNK
                     FIHGGILLDEDDVFVKQIKDETTGLITNRGTKYKIRLTNHIKNLINKDSTNVRLGLVV
                     TESINTIGFSKLKTPIGNIKKVPQMSALSPVGTILYGTNIPVGDPNYANRLKLEIYYT
                     KPD"
     gene            complement(8300..9106)
                     /locus_tag="PK218_05055"
     CDS             complement(8300..9106)
                     /locus_tag="PK218_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPW97908.1"
                     /translation="MEDKRILYVSSEVVPYLAESEVSLMSYDVPKMINDQGGQIRIFM
                     PRYGNINERRHQLHEVIRLSGMNLVVNDLDMPLIIKVASIPKERIQVYFIDNDDYFKR
                     KATFTDEEGSMYPDNDERAIFFAKGVIETVKKLNWVPDIIHVHGWMASVLPVYLKHFY
                     KDEALFSDTKIVISVYGQSFDGTLNNEMINKIAFDNIPLEAIKELETPTYENIIKSAI
                     NNSDAVIVASSSILSSLTNFIESSDKPFLPFAPKEQFANAYTEFYKKMLS"
     gene            9213..10061
                     /gene="panC"
                     /locus_tag="PK218_05060"
     CDS             9213..10061
                     /gene="panC"
                     /locus_tag="PK218_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPW97909.1"
                     /translation="MKIFVTQNELSNHLIPFYNDKSTIGFVPTMGALHQGHLSLLETA
                     KQENSLVIISIFVNPTQFNNPEDLKKYPRTLQSDVEKIKKISTDIIVYAPTVEDIYKG
                     KTVSEHFNFDGLENQMEGKYRTGHFDGVGTIVKELFKIVKPTNAYFGEKDFQQLQIIR
                     KLVSKYKIPVNIIGCPILRETNGLAMSSRNERLSSIERKEASIIYKTLLEAKTLFKTK
                     SAKEVMNWVASVFNENKKFELEYFEIADEDQLLPCNRKNKSKKYRAFIAVFVNNIRLI
                     DTISLN"
     gene            10075..10425
                     /locus_tag="PK218_05065"
     CDS             10075..10425
                     /locus_tag="PK218_05065"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPW97910.1"
                     /translation="MQIEVVKSKIHRVKVTGADLNYIGSITIDEALMDASNLIEGEKV
                     TIVNINNGERFETYVIKGNKNSGEITLNGPAARRVQKGDVIIIISYALMEFEKAKTFK
                     PWLVFPNEEDNSLT"
     gene            10428..11393
                     /locus_tag="PK218_05070"
     CDS             10428..11393
                     /locus_tag="PK218_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPW97911.1"
                     /translation="MKNKIRKWLSILVPLFIGIGIIYYQFSTLTLEEIDKIKISFQKA
                     NYYYIFLSLIIACVGYWSRAYRWKYSLHHLGYQTKFYNNFFTVSVSYLVNLTVPRSGE
                     VSRAALLKKYENIPFDKAFGTIVAERIVDLAIFFLFVFIGFLLQFDKLNRFLIDKNVS
                     LKTIGIFSTVGLVLFIFFILIWIYAEWKIIKKLKKRLAGLTEGMTTIFKMKDKWKYLF
                     HSFFIWFSYLSMFYVSIFALPETSNISFDIVIMGFIFGSLAIGFSNGGLGAYPFSIAL
                     VLSLYGISTESGTAFGWLVWTSQTLLTIVLGLVSYLLLPILNKNK"
     gene            11450..12694
                     /locus_tag="PK218_05075"
     CDS             11450..12694
                     /locus_tag="PK218_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPW97912.1"
                     /translation="MKKILFLVIIAFSATSFSQKKITDTIFSKKLNEKREIFISLPPS
                     YEKNKSQKYPILVLLDGDFLFSAFNGALSYGYYWDDLPEVIIVGINQNNTREADCAVN
                     AETGLPEEKGTQFFEFIGIELIPQIEKNYRVAPFKIVSGLDVTAGFLNLYLYKDQPIF
                     NAYISFSPELPFEMEKNLPQRLATIQQPIFYYHCTADGDLKKMRTRILTMDEEIKKIN
                     KPTLNYKFEDFKDASHYSVVLHAIPSALYQIFSVYQPISMTEFQEKIVKLPSGYAEYL
                     KQKYELLEKSLGLKLQIRLNDFRAIEAAILKNNALNEFDELAQLAEKNYPKSMLADYH
                     MGMMYEKKGDYAKASKTYLRAFQREEIGDLTKDMMYEKSEEMKRLSPKKGKKGEVIEE
                     TPVEENPTEAPTETPTEEKKPE"
     gene            12694..14055
                     /gene="radA"
                     /locus_tag="PK218_05080"
     CDS             12694..14055
                     /gene="radA"
                     /locus_tag="PK218_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPW97913.1"
                     /translation="MAKLKTTFFCQNCGTQYAKWQGQCNSCKEWNTIAEEIIQKEEKS
                     SWKPPTSETKRVSKPLKIKEIDSTQEVRMDTTDGELNRVLGGGIVPGSLILLGGEPGI
                     GKSTLLLQISLKLPYKTLYVSGEESQKQIKMRAERITPESDTCYILTETKTQNIFRHI
                     LEIEPDIVIIDSIQTLHTDYIESTAGSISQIRETTAELIKFAKETNIPVILIGHITKD
                     GNIAGPKILEHMVDTVLQFEGDRNHVYRILRSLKNRFGSTAELGIYEMQGSGLREVSN
                     PSEILISHREEELSGTAIASTLEGMRPLMIEIQALVSTAVYGTPQRSTTGYNAKRLNM
                     ILAVLEKRAGFRLGTKDVFLNVTGGISVDDPAIDLAVVAAILSSNEDIAVGKDVCFAG
                     EVGLSGEIRPVNRVEQRIQEAEKLGFSTIFISKYNKISTKFPGIKVILVSKIEEVVEQ
                     LFG"
     gene            14115..14573
                     /locus_tag="PK218_05085"
     CDS             14115..14573
                     /locus_tag="PK218_05085"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97914.1"
                     /translation="MKTAITFLMLVTLISCTDKNNFKQFEELPESHQWEKNATKTFEF
                     GIDDETKTYSISFLFSHIYGFQFNTIPVSFLLENPDGTTETINKAIVIKEKNGKDIGD
                     CVGDVCDLKSILKENTKLQKGNYKITISNNFNGPYLPNVIGIGIQVEKQK"
     gene            14570..16525
                     /locus_tag="PK218_05090"
     CDS             14570..16525
                     /locus_tag="PK218_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HPW97915.1"
                     /translation="MKSKKQKIILFLKIAASVFVLGLILLFTFRSAVLEKIITRVSTK
                     LENDYQCNFTVKKAAFDGLTSLHFEDISLVPKNQDTLVYIEKLNTKINIWKLIVGDVQ
                     LGKLEVNNGFIQLVKNKKGRNFEAFLKTKKDTLSDSNSNYGKVVNRILSQLLDLIPTD
                     LQVKSFDLKFDDNGNKAVFDCTEMVLHDKKFSSTIVVKSDLKEQLWNVSGFANPRDRK
                     TDLILSTSENDTIKIPFINKKFNLKTGFKSIHFILDEMDLSHGNLHLKGIASVQNLLI
                     NHPKISKKDVLVRNARMEYHFVFGERFVAVDSTSKIQINKIILKPYLSYENDKDKTYS
                     LQLKIPKIKAQDFIESLPNGLFNHLEGMKAVGNFDYKLNFKYNNHHPDKLIFESNLKP
                     DKLKISQFGEANLNKINDEFVYRAIEKGIPQRPIIVGHANPFYTPLHEISPYLQKCVL
                     TSEDPSFLQHRGFINDAFKQSIAKNIRTKKFSRGASTISMQLVKNVFLTREKTLSRKL
                     EEIMLVYILENQRITSKERMLEVYFNVIEFGPNVYGIGEAAAFYFQKQPKELTLNQCL
                     FLATIIPRPKGFMYKFDGNQSLKISSKQENKFLTNVMLRRKVLEPNDTLGQHLPLKIS
                     GQAQFFLKNNAFSKTEIDSTSSDEFDF"
     gene            16577..17959
                     /locus_tag="PK218_05095"
     CDS             16577..17959
                     /locus_tag="PK218_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPW97916.1"
                     /translation="MKKILLSLLLLFSFFINAQTVAIQSFATGFSSAVAIVHPPNDSR
                     LFVVQKTGAIRILNTNGTINTTPFLTITGLTSGSEQGLLGLAFHPNYASNGQFFVNYT
                     NSSGDTVIAKYTVSSNPDVANTTSTTLLTIDQPFSNHNGGSLVFGSDGYLYIGMGDGG
                     SGNDPNGYAQNLTVDSSNPSRIFLGKILRLDVDSASPYGIPATNPFVGQAGKEEIWAY
                     GIRNPWKFNFNRLNGDLWIADVGQDAVEEIDKVSNPLPNTGLNFGWRCYEGTSSNITG
                     GCPAFSSTVAPFTQYNQTTARCSITGGYFYTGSMYPNFANKYFFADYCSGEIGLVSST
                     GSITWALDTPSVITSFGEDVNGELYITNGSTVSKIIDTSLSNTNFDKNTIRVYPNPAK
                     TQITIDNSSNTTFNAIQLIDITGKILYAENNLNATSKTINTSHFTAGLYIVVAEDLNG
                     NQFKSKVIIE"
     gene            complement(17984..19018)
                     /locus_tag="PK218_05100"
     CDS             complement(17984..19018)
                     /locus_tag="PK218_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HPW97917.1"
                     /translation="MIYTKLPNTDIKISKVCLGTMTFGEQNTENEAHSQLDYAIERGI
                     NFIDTAEMYPIAARKQTLGATEKYIGTWLKKTNYREKIVLATKIAGPNRGMDYIRQPL
                     DFSKKSIHQAVDKSLKNLQTDYIDLYQMHWPERVMNMFGQRGVATIDKEWRYNFLEVL
                     TIFDGLIKDGKIKNIGVSNENPYGIMKYISESEKYNLPRIITIQNPYSLLNRIYEIGL
                     SEIGMRENIGLLGYSPLAFSFLTGKHLKGIQSNSRLGLFPNFTRYTNDNCFKATQFYK
                     TIAERNGMTLTQMALAFVNQQQFVTSTIIGATTMEQLKENIDSFEIKLSLNVINEINK
                     IQELYPNPAP"
     gene            complement(19622..19837)
                     /locus_tag="PK218_05105"
     CDS             complement(19622..19837)
                     /locus_tag="PK218_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW97918.1"
                     /translation="MVEKILIKIKKRRNELGYSQHYVSSQLNITQSSYNKIENGTTEL
                     SVAVLMKLCQILQLEYESMHFDEKDAV"
BASE COUNT         6663 a         3413 c         3078 g         6830 t
ORIGIN      
        1 agctttcaaa aattcatttg caattaatgc atatcctctt gggcttggat gaattccgtc
       61 taaagaaaaa gcagctccag taacaaaagt agaagttaat gtataattat tggctacaat
      121 tcctgtagta cttaactttt gcataatcgc ttttgcgtca acaaaagcca acccatttgc
      181 atctgctgca ttttttatag taatattata agcatcagtt gcgttactaa tctcaacaat
      241 ttcagaaggt attagaacgt gcctatcttc taatggatag gtaatgccat atttccctaa
      301 atcagcagga acaccaggag gtaaagctgc atctgtccca ataactgaag aagttactaa
      361 aggaattaaa tcacctgttg ttgtttgtct aactttacca aatgtagcac ctaagtaact
      421 agacaaagcg attaattgag ggttaccact tcctaaagcc gctccagtaa tttgggcacc
      481 ataatcagcc agttcatcat ctttcatcaa aactgggttt gcagttgttg tagataaaac
      541 attaattctt gaaccttgac catacgctgt taggacttgg tctaaaaatc ctaaaatccc
      601 attgattgta ttaatagttg caacatcccc cacactaact actcttgatg tcttgttttc
      661 atccccatca acataatact taaatggatc tacaacagca tttggattta tgtatgtgaa
      721 ttgtggtagc gttgtaatat atggtaaatt agcaacaact ccttttcttc caccagtgac
      781 aagttgacta acaatttcat tataagaggc atcaaaacca acaccaattc caccgctaga
      841 tggtgttatt ggattcaacg tagaatctcc tccagctaat gcgtaaccca aaacatcatt
      901 ccctccaatc cataacgaaa agaaagttgg tgattgactt acggcatcat tcagcactgt
      961 tgtagaagga gaagtagcaa aacgagcaaa ataagggttc ccaaaactac ttccatatcc
     1021 aggagtaact aaatgaatgc atttcgctcc tggaatacct agattgccat acaatcctga
     1081 taacatgctt gaaatatctg tagaagaaac accatcaacc ttgaggggtt catcaaaatc
     1141 agaaccttct ttcttgccaa aatataatct cactccataa gcggagcttt gcattccgcc
     1201 acttgaaaaa cctccaatat tatccgccat aaatggaatg ttgaaatcgc caccaccaac
     1261 taaagcaaat tgagaagcta ttatgtttgg ataggaattt ttttgccctt ccataaacaa
     1321 agcattatct gaatacccag ctgcaaaaga atctcctaaa gcaacatatt ttgaaaaatc
     1381 tgcacttcct gcagttaaag gtgttccatc tgttgaattt ggatcaacaa taacctcatc
     1441 atcgctatta caagctacaa atgctaaagc tacaaatgcc aaccatttta tattttttat
     1501 cattttcata atttttttaa ttaaggatta atagttaatg aaacgaaata ctgttgtccg
     1561 attaatccag gaccaagaag ttgtctgtat tcttttccac caagattagt tgcgcccact
     1621 tttaacgttg attttaacgt tggaattcca taactaagtt gagcatcaat tactgtggca
     1681 gcatcaatca ttccatcggc aaatgttgac tcccataagt attcatcact ccatcttgcg
     1741 cttacattaa atccaaagtt tttaaacacg ttgtcattgc taattgaacc tttcactctg
     1801 tgttttggag tgttaaatcc tgcttcgaaa cttgggtctt ttgcttgatc aaaatcaaat
     1861 tgagcataat tataatttgc agaaacctca aagtttttag gtaatttttt agacaaccct
     1921 acaccaaacc ctaatgattt gatttcgata tcggtgtttg tgtatagttg gaaagctcta
     1981 taattacccg attgtaaagc ttgaagagat tgaacttcag gattgttttg atttgggtca
     2041 gcagataagt atggaactgg atttgtagct ccttttgtaa aatcaaaagt agttcctgct
     2101 gttccgtata atggtgctaa tacggttaag tttcctataa aattattata aatattatag
     2161 tatccattaa tatcaatatt aaaaccttca tattgacctc tatatcctaa ttcaaatgct
     2221 tttactgttt ctggtttcac tagaccaaca ttcgtttttt ctaataatgc agccgctcct
     2281 ggtaaattgc ctggatttgc agaagcaaat gcaccaaaag ccgttacaga tgctgacgtg
     2341 tatgaattat aataagcatt attaccattt attgtagtac ttgtagcagg atttaaaggg
     2401 tctccactag cgtaaatttg acctattgtg gttgtaacag gtaacgtttc actatatcga
     2461 gttaaattat ctggagcaga acctaaaaga attgcatttc caacattgaa tccaatatat
     2521 tggtcttgtg ttgttgggtt tcggaaacct gtttggaacg aacctctaaa cacatggttt
     2581 ttactttcac ctgcagagta aactgctgat attcttggag aataatttcc gtcaaaatta
     2641 tccgatttgt cataacgaac agaaccagtt aacttcaatc ggtcatccat taattttttc
     2701 tgaacttgag tatatactcc atattcatca tatttaattc ctccatattg cggtgtatct
     2761 gtataaattc taccaaaaga atttaagtca tatcttctat atgaaccacc aacttgaatt
     2821 tcagcaaatt ttactaaatc tttaaaatta tagttggcat ctgaatgata aatttttgaa
     2881 ttatctacca atttagaacc cgaaagcaca tctggattag caattacatt tttaaatgca
     2941 ttttgaaact ctgctgttcc tggaaggaat cttcctgtat ctgccacacc tcttgccgta
     3001 gcgtgtgctt gttgtggtgt tgctcctgct agtgtagatt gcacataagc accagcatac
     3061 tgaccaaacc aagtcttatc atctttccaa gctctgttaa tatttagtcc tgtaaatagc
     3121 atatcatatg aatttccgcc gtcttcagta gtaaaataac ctcttaagaa aaagtttttc
     3181 cctgtaaatt ccaatttgtg ttgttgcatt gtaaacccat tcaaatagta tcgattggct
     3241 ccttgatata cagagtttcc aaatccaaat ttactttgcc atatgatttc aaaattctca
     3301 tttcctcctg gtctgaaatg taaagagaaa tctactttgg catttttaac aacattatct
     3361 gttaaatcaa cttcattata ccccgttcta gatacatttt catttggcaa taaatttaat
     3421 gcagaagcag gtaacaaacc taagcccact aatgtttgtc caacgctttt gatgtttgta
     3481 gtagcttcat caccataaac gttcatacca ttataatttg gatggcttct atctcttcct
     3541 tcaaccgttt tgtcgctata atcagtagcc caccattctg ttcctttcat aaaagtaaag
     3601 ttggctttgg ctgcaaaata atcgttgaat ttatgtgctg ctcttatacc ataatcatag
     3661 tataaattag caccagctgc tttttgattg gtttgaccgt atttagcata tacagaaatc
     3721 ccttggtctg taaatggatt tttacttttc atgaataaaa ttccattaaa agcatttgct
     3781 ccatataaag cagatgatgc tcctggaagt aactcaacac tagccacgtc aatatcagaa
     3841 acaccaataa ggtttcctaa tacaaagttt agcaatggag aagagttgtc cattccatcc
     3901 actaactgca taaaacgggt attggcaaca gttgcaaatc cacgagtatt aatcgatttg
     3961 aacgacatac tactagtgtt catttgcact tcttttaagt tctccaaacc gtcatagaaa
     4021 gttggcgaag cagttttttt aacatcagca gccgtaaatc tttcgattgt aactggcgac
     4081 tctcgcaatc gttctggtgt tcgagaagct gaaactacaa tttcatctaa taacattcgt
     4141 tcatcactaa gaacaactgt tactttttga ttgttagttg aaatagaaac cattttttca
     4201 ccatatccta tacttgaaat tttaatttca aaaggtggtt tcttggatgt ggataagata
     4261 aaatttcctt cattgtctgt aatagttcct gtagattctc cagaaacggt aacatttgct
     4321 cctggcaatg gctcattatt accatctttt accgaacctg taatagttgt ttgcgcaaat
     4381 gaaatgctac aaaaaaacaa tgaaataata aataaataaa ttttcatttg gtttttaatt
     4441 ttgttggttt aagtagccaa agtacaaata ttttttttag attattaata atcgatgctt
     4501 atttttattg aattataaat atttctcaat taaattattg aattctaaat ctataaaaat
     4561 tatgtttttt ttaacaaaaa tactgtttcg ataaaaaaaa cactatgcgc gcatataaag
     4621 ttgttaaaat tttatagatt cttcaaaaaa ccccaataaa aaagcgagat gtaattctcg
     4681 cttttttaaa atattatttt caaaaaattt actcaaccgt aactgatttc gccaaatttc
     4741 ttggttggtc aacattgcat tctctcataa ccgcaatatg ataagacaat aattgcaatg
     4801 gaatagttgt aattaatggc gataatgcat ccgaagtttc agggatttcg attacgtggt
     4861 cagccaattg ttttacttgt gtatcacctt tggtaacaat agcaataatt tttccacttc
     4921 ttgatttaat ttcttgaata ttacttacca ctttatcata atgaccttgt tttggtgcaa
     4981 ttacgattac tggcattttt tcatcaatca aagcaattgg tccgtgcttc atttctgctg
     5041 ctggataccc ttcagcatgt atataggata tttcttttaa ttttaatgcg ccttctaaag
     5101 caactgggaa attataacct cttcctaaat acaaacagtt tggcgcatcc ttataaattg
     5161 atgcaatttc ttttgcgata tcgtttgtct ttaatgcttc ggctactttt tcaggaataa
     5221 tttctaattc ttgcaaatac ctgaagaaat ctgaattagt aagtgttcct tttgcttttg
     5281 ctagtcgaag tgctatcata gctaaaactg tgatttgtgt tgtaaatgct tttgtagaag
     5341 ctacaccgat ttctggacct gcatgggtat atgcacctgc atgagattct cttgaaatag
     5401 aagaacctac tacattacaa actccaaata caaaagcacc tttttcttta gcaagtttta
     5461 tggcagccaa agtatctgct gtttctcccg attgagaaat ggctattaca atatctttag
     5521 cggtaatgat tgggtttcta tatctaaatt cagaagcata ctctacctca acaggaattc
     5581 tagcaaattc ttcaattaca tattctgcaa ctaaacctgc atgccatgac gttccacagg
     5641 caactattaa aattctttcg gcgtttaaga atttttcaag attgtcttca acacccgaca
     5701 tctggataag accttggtta gaatgaagtc ttcctctgta agtgtctttt ataacattag
     5761 gttgctcgta aatttcttta agcataaagt gatcgtatcc tcctttttca atttgctcca
     5821 aattcatttg aagttcttga atataaggat caaccatgga atcatcttta atttttctaa
     5881 tttttaatgg cttatgtctt cttatgactg ccatttcttc atcttctaaa tagatggcgt
     5941 tagaagtata ttcgataaaa ggtgatgcat ctgatgcaat aaaatactcg tcttttccta
     6001 cgccaatagc cagtggactt cctaatcgtg ccacgacaat ttcatctggt ttttctacat
     6061 caaaaacaca aatagcatac gctcccacaa cttggtttaa ggctacttga acagctttac
     6121 ctaatttaag tttttctttt ttctgaactt cttcaattag atttacaaga acttcagtat
     6181 ccgtatctga tttaaaaaca taacctctat taataagttc tttttttaaa ggttcgtaat
     6241 tttcaataat tccattatga ataatggcaa ttttaccaga gtttgataaa tgtggatgtg
     6301 aatttacatc atttggcaca ccatgtgtag cccaacgggt atgtcccatt ccaatagtag
     6361 cgttagcatt tgctatgtcg attgactttt cttcaaggtc agaaactttg cctttggttt
     6421 tagaaacttt taaatcattt ccatcaaaaa gcattattcc cgcactatca tagcctctgt
     6481 attctaatct ttttagtcct tttatcacta tagggtacgc ctcgcgatgt cctatatatc
     6541 caacaattcc acacatagtt ttttgtattt agtcaggttt ggtataataa atttccaatt
     6601 ttaatctatt cgcataattt ggatctccaa caggaatgtt ggttccatat agaattgttc
     6661 caactgggct gagcgccgac atttgtggta cttttttaat atttccaatt ggtgttttta
     6721 attttgaaaa cccaatggtg ttaattgact ctgttacaac cagccctaat cgaacattag
     6781 tagagtcttt atttattaga tttttaatat gatttgttaa tctaatttta tatttagttc
     6841 ctctattggt tattaaccct gttgtttcat ctttaatttg cttaacaaaa acatcatctt
     6901 catcgagcaa aatcccgcca tgtataaact tattctgttt aggatttcta ctgtttagac
     6961 tttggtcagt taaataatct agcaagactc tattgttatt taaatcgtat aacattaatc
     7021 tattgggttc aatagcttga ccagttatcg ctgttttatc aatatagaag gttaaattag
     7081 cttcgtttat aagccatccg tttgctttaa tttgttctac ttcatctgca attccattat
     7141 tgtcagcatc gggtccaaac aaatcaatta ttgctattga gccttctccc ccttttaaat
     7201 ataattttga agcttcttga gtagcattag cagcactcaa ataatccatg ttttcattag
     7261 aattggattg taaactcact cggtttcctg caaaatttaa atcaaacgtt ttattctttt
     7321 tttcaaaagt agtagtactg ccaatggtaa cttttaaatc ttcagtataa tataccgtaa
     7381 cttttgcgtt cgcattagcg aaattcaaca ttgccatact gtttgaattt cctaaatctt
     7441 caactttaaa atacaatcct cgaaaataat ttttgaaaat tgtattgctt gccaattgac
     7501 tagttggtgc atttattatt ttattataaa aagttgcttt gtctaaatgc aatcgcaaag
     7561 atggtactga tcttgtagga acatcaacat tttcagaatt tttaactgtt gttttatgtt
     7621 ctcttttgtc gaaataaaac tgagcatttt catgtccatc taaattttta tcatcaaaaa
     7681 cagataagtt atttaaaaca aaaggtattt tttgattttc aatttcttgc atatcattgt
     7741 aaaacacttg ttgctccgta aattgagctg taggatctaa atctctcaaa aaatagttag
     7801 attcataaac gcttaatctc atttttgaac cagcttcacc atagatggag tccaactgat
     7861 aagtactaat accttctgag gtgacagtac caataacttt actataataa ggtacattta
     7921 aaataacact atcaataatt ggcaaatctt gataaagagt gctatcggta ttattaaatt
     7981 ttctgccaat tgaacccgaa gccatttcca attgtgacac aaaaccagcg gtagttgttc
     8041 caaaaactgg atttttataa atacctaatg gatttatcgg caaattattc atcgcaacag
     8101 caccaatttt ttgattgtat gctttaatag aagcattttc atatctatca aacagatagt
     8161 ggtcattgtc accaccaacg atgttagttc caatttcgtt aaaatcttta tcgcaagaaa
     8221 tcatcattgc gaaaatagta aaactaaaaa atctaagtaa ataagattta aaattcatct
     8281 ttaatttata tataaatatt tatgaaagca tttttttgta aaattcagta tatgcatttg
     8341 caaactgttc tttcggggcg aaaggtaaaa aaggtttatc cgaagattca ataaaatttg
     8401 ttaaacttga caaaatactc gatgaagcga caattactgc atcagaatta ttgatggcac
     8461 tctttataat gttctcataa gtaggggttt caagttcttt tatcgcttct aatggaatat
     8521 tgtcaaaagc aatcttgtta atcatttcgt tgtttaaggt cccatcaaaa gactgaccat
     8581 atacagaaat tacaatttta gtatccgaaa acaaagcttc gtctttgtaa aaatgtttta
     8641 aataaaccgg caaaacgctt gccatccaac cgtgaacatg aataatatct ggtacccagt
     8701 ttagtttttt aacggtttct ataacacctt ttgcaaagaa aatggctctt tcatcattat
     8761 cgggatacat gctgccttct tcgtctgtaa atgtagcttt tcttttaaaa taatcgtcat
     8821 tatcaataaa ataaacctga attctttctt tgggtattga tgctacttta ataatcaaag
     8881 gcatatctaa atcatttacc actaaattca tcccagacaa acgaataacc tcatgcaatt
     8941 gatgccttct ttcgttaata ttgccatatc taggcataaa aattcttatc tgtcctcctt
     9001 gatcgttaat catttttgga acatcataag acattaaaga aacttcactt tctgccaaat
     9061 aaggcactac ctcagatgat acatataata tcctcttgtc ttccatattt tacttttctt
     9121 tatttattgg cgttaaaaaa catgcaaaag tacaaaaaat tgcgcagtta tatatgaaaa
     9181 tagtaagttt gcaccctaac taaagataat tgatgaaaat tttcgtaact caaaatgagc
     9241 tgagtaatca tcttatacca ttttacaatg ataaatcaac aattggtttt gtgcctacaa
     9301 tgggtgcttt acatcaaggt cacttgtcat tgttggaaac agcaaagcaa gaaaattctt
     9361 tagtgattat aagtattttt gtcaatccaa cacagtttaa caatcctgaa gatttaaaaa
     9421 aatacccgag aacgttacag tctgatgttg aaaaaataaa aaaaatctca accgatataa
     9481 ttgtttatgc tccgacggtt gaagacattt ataaaggaaa aacagtttca gaacatttta
     9541 attttgatgg tttagaaaac caaatggaag gcaaatatcg aacaggacat tttgacggtg
     9601 ttggaacaat cgttaaagaa ttatttaaga ttgtaaaacc tactaatgcc tattttggag
     9661 aaaaagattt tcaacaattg caaatcatcc gaaaattggt ttcaaaatat aaaattccag
     9721 taaacataat tggttgccca atattaagag aaacaaacgg acttgcaatg agttctagaa
     9781 atgagagact gtcatcaatt gagcgaaaag aagcctctat tatttataaa acgctattag
     9841 aagcaaaaac gctctttaaa actaagagtg ccaaggaagt tatgaattgg gttgcctctg
     9901 tatttaatga gaataaaaaa tttgagcttg aatattttga aattgctgat gaagaccaat
     9961 tgcttccttg taatagaaaa aacaaatcaa aaaaatatcg tgcttttata gccgtgttcg
    10021 taaacaatat cagattaatc gatactattt cattaaatta attatttttg ccccatgcaa
    10081 attgaagttg taaaatcaaa aattcaccgc gtaaaagtta ccggagccga tttaaactat
    10141 atcggaagta tcactattga cgaagctcta atggatgctt caaatctcat tgaaggcgag
    10201 aaagtgacta ttgttaatat taataatggg gaacgttttg aaacctatgt tatcaaagga
    10261 aataaaaact caggtgaaat cacactaaac ggtccagctg ctagacgcgt tcaaaaagga
    10321 gatgtcatca ttataatttc ctatgcctta atggaatttg aaaaagcaaa aacctttaaa
    10381 ccatggttgg tattccctaa tgaagaagac aattcattaa cttagtcttg aaaaataaaa
    10441 ttcggaaatg gctgtctatt ttagttcctc tttttattgg aattggaatc atttattatc
    10501 aattttcaac attaacttta gaggaaattg ataaaataaa aatcagtttc caaaaagcaa
    10561 attattatta tattttttta tccctaatta ttgcttgtgt tggctattgg tctagagcct
    10621 atcgttggaa atattcatta caccatttag gatatcaaac gaaattttac aataattttt
    10681 ttaccgtttc tgtatcctat ttagtcaatt taactgttcc ccgctctggt gaagtttctc
    10741 gtgcagcttt actaaaaaaa tacgagaaca ttccttttga caaagctttt ggaactatag
    10801 ttgccgaaag aatagttgat ttggctatct ttttcctgtt tgtttttatt ggttttttat
    10861 tgcaatttga taaattaaat agatttttaa ttgataaaaa tgtatcccta aaaaccattg
    10921 gtattttttc tacagttggt ttggttttat tcatattttt tattctaatt tggatatacg
    10981 cagagtggaa aattataaaa aaattaaaaa agagattagc tggcttaacc gaaggaatga
    11041 caactatttt taaaatgaaa gataaatgga aatatctttt tcattctttt tttatatggt
    11101 tttcgtactt atcgatgttt tatgtttcaa tttttgcatt gccagaaact tcaaatatta
    11161 gttttgatat cgttattatg ggatttattt ttggcagttt agctattggt ttttctaatg
    11221 gtggattagg agcttatcct ttttcaatag ctttagtcct atcgttatac ggaatatcta
    11281 cagaatccgg tactgcattt ggatggttgg tatggacttc acaaacactg ctaactatag
    11341 tccttggttt agtttcttat ttattattgc caattttaaa taaaaataaa taaattagta
    11401 tcttgctaac tattttagca aagctaatta ttgacctaaa ccaatcttta tgaaaaaaat
    11461 acttttttta gtaataatcg ctttttcagc aacttctttt tctcaaaaaa aaattactga
    11521 cactattttt tcaaaaaaac taaacgagaa aagagaaatt tttataagtc ttccaccttc
    11581 ttatgaaaaa aacaaatctc aaaaataccc aatattagtg cttttggatg gcgattttct
    11641 ttttagtgcc tttaatggtg ctttgagtta tggctactat tgggatgatt taccagaggt
    11701 aattatagtt ggcatcaatc aaaacaatac tcgagaagct gactgcgctg ttaatgctga
    11761 aacggggctg cccgaagaaa aaggaaccca gttttttgaa tttattggca ttgaactaat
    11821 cccacaaatt gaaaaaaact atagagtcgc accttttaaa attgtttcgg gcttagacgt
    11881 tactgctggt tttttaaatt tatatcttta caaagaccaa cctattttta acgcttatat
    11941 ttcttttagt ccagaattgc ctttcgaaat ggaaaaaaac cttccacaac gattagctac
    12001 cattcaacaa cctattttct actatcattg tactgctgat ggtgatttaa agaaaatgag
    12061 aactcgaatt ttgacaatgg acgaagaaat aaaaaaaatc aataaaccaa cgctaaatta
    12121 taaatttgaa gactttaaag atgcttctca ctattctgtt gttttacatg caatcccaag
    12181 tgctttgtat caaatattct ctgtctatca acctatttca atgacggagt ttcaagaaaa
    12241 aattgtcaaa ttaccttcag gatatgctga atatttgaaa caaaaatatg aattattaga
    12301 aaaatcatta ggactaaaac ttcaaataag attgaatgat ttcagagcaa ttgaagctgc
    12361 cattttaaaa aataatgcgt taaacgaatt tgacgaacta gcacaattag ctgaaaaaaa
    12421 ctatccaaaa tcgatgttgg ccgattacca catgggaatg atgtatgaga aaaaaggcga
    12481 ttatgccaaa gcctctaaaa cctatttaag agcatttcag agagaagaaa ttggcgattt
    12541 gaccaaagac atgatgtatg aaaaatcaga agaaatgaag cgtctttctc ctaaaaaagg
    12601 caaaaaaggt gaagtaattg aagaaactcc tgttgaagaa aacccaacgg aagcaccaac
    12661 tgaaactccg acggaagaga aaaaaccaga ataatggcga aattaaaaac gacctttttt
    12721 tgccaaaatt gtgggactca atatgcaaaa tggcaaggcc aatgcaactc gtgtaaagaa
    12781 tggaatacca ttgccgaaga aattattcaa aaagaagaaa aatccagttg gaaacctcca
    12841 acttctgaaa ccaaacgcgt ttcaaaacca ttaaaaatta aagaaatcga ttctactcaa
    12901 gaagttagaa tggatactac tgatggagaa ttgaatagag tgcttggtgg tggaatcgta
    12961 ccaggttctt taatccttct tggtggcgaa cctggtattg gaaaaagcac tttgttgcta
    13021 caaatttcgt taaaactacc ttataaaacc ttatacgttt cgggcgaaga aagtcagaaa
    13081 caaattaaaa tgcgcgcaga acgtattact cccgaaagtg acacctgcta cattttaacc
    13141 gaaaccaaaa cgcaaaatat ttttcgacac atacttgaaa tcgaacctga tattgtcatt
    13201 atcgattcta ttcaaacctt gcacacggat tatattgaat caaccgccgg aagtatttct
    13261 caaattagag aaactaccgc agagctgata aaatttgcca aagaaaccaa cattcctgtc
    13321 attctaattg gtcatataac caaagatgga aacattgctg gaccaaaaat tttggaacac
    13381 atggtcgata ccgttttaca gtttgaaggc gatagaaatc atgtatatag aattcttcgt
    13441 tcgcttaaaa accgttttgg ttctactgcc gaattgggaa tttatgaaat gcagggttct
    13501 ggtctgcgag aagtttccaa tccatccgaa atattaattt cacatcgaga agaagaactt
    13561 tcgggaacag caattgcctc aactttggaa ggaatgcgac cattgatgat agaaattcaa
    13621 gctttagttt ctacagcagt ttatggaact ccgcaacgaa gcacaacggg ttataacgcc
    13681 aaacgattga acatgatttt ggctgtatta gaaaaacgcg ctggttttcg attgggaaca
    13741 aaagatgttt tcttaaatgt aactggtgga atttcggtgg atgatccagc catagattta
    13801 gccgttgtag cagccattct ttcttctaat gaagatattg cagttggtaa agatgtttgt
    13861 tttgctggtg aagttggtct ttctggcgaa attcgtcctg taaaccgtgt agaacaacgc
    13921 atacaagaag ccgaaaaatt aggtttttca acaattttta tatccaaata caacaaaatt
    13981 tctaccaagt ttcccggaat taaagtaatt ttagtttcca aaattgaaga agttgtggag
    14041 caattgtttg gataaattta attttttaaa tactaaaacc ccacaaaatt cgttataact
    14101 ctaaacctat aattatgaaa acagcaataa cttttttgat gttagttacc ctaatttctt
    14161 gtactgataa aaataatttt aaacaatttg aggaattgcc agaaagtcat caatgggaaa
    14221 agaatgccac aaaaactttt gagtttggga ttgacgatga aactaaaaca tattccattt
    14281 cttttttatt tagtcatatt tatggctttc aattcaacac tattcctgta agctttcttc
    14341 ttgaaaatcc agatggaact accgaaacta ttaataaagc tattgtaatc aaagaaaaaa
    14401 acggaaaaga tattggcgat tgtgttggcg atgtttgtga tttgaaatct atacttaaag
    14461 aaaacactaa acttcaaaaa ggaaattata aaatcaccat ttcaaataat tttaacggtc
    14521 catatttgcc aaatgtaatt ggaattggaa tccaagttga aaaacaaaaa tgaaatctaa
    14581 aaaacaaaaa ataatactat ttctaaaaat tgcagctagc gtttttgtac ttggtttgat
    14641 tttattattt acttttagaa gtgccgtttt agaaaaaata ataacccgtg ttagcacaaa
    14701 attagaaaat gactaccaat gcaattttac ggttaaaaaa gctgcttttg acggactaac
    14761 ttctttacat tttgaagaca tttctttagt gcccaaaaat caagatacat tggtttatat
    14821 tgaaaaacta aatactaaaa taaatatttg gaaattaata gtcggcgatg ttcaactcgg
    14881 aaaattagaa gtcaacaatg ggtttataca attagtaaag aataaaaaag gtcgtaattt
    14941 tgaagctttt ctaaaaacta aaaaagacac actttcggat agtaacagta attatggcaa
    15001 agtagtaaat cgcattttat cccaactttt agatttaatt ccaaccgatt tacaggtaaa
    15061 aagttttgat ttaaaatttg atgacaatgg aaacaaagca gtttttgatt gtaccgaaat
    15121 ggtcttgcat gacaagaaat tttcgtccac cattgtagta aaatcagatt taaaagaaca
    15181 actttggaac gtttcgggtt ttgccaatcc gcgtgataga aaaacagatt taatcctttc
    15241 tacttctgaa aacgacacta taaaaattcc ttttataaac aaaaaattta atctcaaaac
    15301 aggtttcaaa agcattcatt ttattttaga tgaaatggat ttgtcacatg gaaaccttca
    15361 tttaaaagga attgcatccg ttcaaaacct acttattaat catcctaaaa tatctaaaaa
    15421 agatgttttg gtaaggaatg ctagaatgga atatcatttt gtttttggtg aacgatttgt
    15481 agcagttgat agcacttcta aaattcaaat caataaaatc attttaaagc cttatttgtc
    15541 ttatgaaaat gataaggata aaacctattc attgcaattg aaaataccaa aaatcaaagc
    15601 gcaagatttt attgaatcat tgcccaatgg tttgtttaat catcttgaag gaatgaaagc
    15661 tgttgggaat tttgattata aactaaattt taaatacaac aatcatcatc cagataaatt
    15721 gatttttgaa agtaatttaa aaccagataa attaaaaatc tcacaatttg gtgaagccaa
    15781 tttgaataaa attaatgatg aatttgtcta tcgcgctatt gaaaagggaa ttccgcagcg
    15841 accaattata gttggacatg ctaatccttt ttatacacca ttacatgaaa tttctcccta
    15901 tcttcaaaaa tgtgttttga caagcgaaga tccatcgttc ctgcaacatc gtggatttat
    15961 aaatgatgct ttcaaacaat cgattgcaaa aaacatcaga accaagaaat tttctcgtgg
    16021 tgctagtaca attagcatgc agttggtaaa aaatgtgttc ttaacaagag aaaaaacatt
    16081 atcgagaaag cttgaagaaa tcatgttggt ttacatactc gaaaatcaaa ggataacctc
    16141 taaagaacga atgctggaag tgtattttaa tgtaattgaa tttggaccaa atgtatatgg
    16201 tattggcgaa gcagctgctt tttattttca aaaacaaccc aaagagttaa ccttaaacca
    16261 atgtttgttt cttgccacaa ttattccaag accaaaaggg tttatgtaca aatttgacgg
    16321 aaatcaatct ttaaaaatat cgtcaaaaca agaaaataaa tttttaacca acgtgatgtt
    16381 gcgaagaaaa gttttagaac caaatgacac ccttgggcaa catcttcctt taaaaataag
    16441 tggtcaagca cagttttttc taaaaaacaa tgctttttcc aaaacagaaa ttgatagtac
    16501 ttcaagtgat gaatttgatt tttaaatacc cgatgttaac ctttttattc ttaactttac
    16561 aaaaaacatt tttgttatga aaaaaatttt actttcactt ttactactct tttctttttt
    16621 tattaacgca caaactgttg ccatacagtc ttttgcaact ggattttcgt ctgcagtagc
    16681 aattgttcat ccaccaaacg attctcgact ttttgtggtt caaaaaacag gtgctattcg
    16741 aattttaaac accaacggaa ccatcaatac aactcctttt ttaaccatta ctgggttgac
    16801 tagcggaagt gaacaaggat tattgggctt ggcttttcat ccaaattatg cttctaacgg
    16861 tcagtttttt gtgaattata ctaattcttc tggtgatact gttattgcaa aatatactgt
    16921 ttcatcaaat cctgatgttg caaatactac tagcacaact cttttaacga tagaccaacc
    16981 tttttctaat cacaatggtg gaagcttggt atttggttct gatggttact tgtatatagg
    17041 aatgggcgat ggtggtagtg gaaatgatcc aaatggatat gctcaaaact taacggttga
    17101 cagttccaat ccaagtcgta ttttcttggg caaaatactt cgattagatg tagattccgc
    17161 tagtccttat ggaattccag ctacaaatcc tttcgttggt caagcgggta aagaagaaat
    17221 ttgggcatat ggtattcgta atccttggaa atttaatttt aatcgtttga atggcgattt
    17281 gtggatagcc gacgtaggtc aagatgctgt ggaagagata gataaagttt ctaatccgtt
    17341 acccaatact ggtttaaact ttggatggag atgttatgaa ggaacaagta gtaatattac
    17401 ggggggttgt cctgcttttt ctagtactgt tgcaccattt actcaataca atcaaacaac
    17461 cgccagatgc tctataacag gcggttattt ttatactggt tcaatgtatc ctaattttgc
    17521 taacaaatat ttctttgctg attattgttc aggtgaaata ggtttagtta gttcaactgg
    17581 ctcaataact tgggcactag atactccaag tgtaatcact tcttttggtg aagatgtaaa
    17641 tggcgagttg tacattacaa acggaagtac tgtttctaaa atcatagaca cttctttgag
    17701 taataccaat tttgacaaaa acacaatacg tgtttatcca aatccagcaa aaactcaaat
    17761 tacaattgac aactcatcaa atacaacatt taatgctatt caacttatag acattactgg
    17821 aaagatttta tatgcagaaa ataatttaaa tgcaactagc aaaacaatca atacttcaca
    17881 ctttactgct ggattatata ttgtagtggc agaagatttg aatggaaatc aatttaaatc
    17941 aaaagttatt atagaataaa aaaactaaaa atcatatttg gtattatggt gcaggattcg
    18001 gatataattc ttgaatcttg ttaatttcat tgatgacatt taaagagagt ttaatctcaa
    18061 aagaatcaat attttctttt aattgctcca tggttgtggc accgattatg gtcgaagtta
    18121 caaactgttg ttgatttaca aaagccaacg ccatttgcgt aagtgtcatt ccatttcgct
    18181 cggcaatagt tttataaaat tgagttgctt taaaacaatt gtcattcgta tatcgagtga
    18241 aatttggaaa caagccaagt ctcgaattcg attgaatgcc tttcaaatgt ttacccgtca
    18301 aaaaactaaa agccaaaggt gaatagccta ataagcctat gttttcgcgc atacctattt
    18361 ctgacaatcc aatttcatat attctattca ataacgaata gggattttga atggttataa
    18421 ttcttggtaa attatatttt tcgctttctg aaatatattt cattatacca taaggatttt
    18481 catttgaaac accaatattc ttgatttttc catctttaat cagtccgtca aaaatagtaa
    18541 gcacctctaa aaaattataa cgccattctt tatctatagt agcaacacct ctttgcccaa
    18601 acatattcat aaccctttct ggccaatgca tttggtataa atcaatgtaa tctgtttgaa
    18661 gattttttaa acttttatct accgcttgat gaatactttt ctttgaaaaa tctagcggct
    18721 gacgaatata atccatgccg cgatttggac ctgcgatttt ggttgcaaga acaatttttt
    18781 ctcgataatt tgtttttttt aaccaagttc caatatactt ttcggttgca cccaaagttt
    18841 gttttcttgc tgctattgga tacatttcgg cagtgtctat aaaattaata cctctttcga
    18901 tagcataatc taattgtgaa tgtgcttcgt tttcagtgtt ttgctcacca aaagtcatgg
    18961 tgccaagaca aactttactg atttttatat cggtattggg taatttggtg tagatcatta
    19021 gaagttctta gggattgcaa atataattat gattaaaaaa cttttatact aatcaaggca
    19081 aaatatttat taaactttgt tgccttgtac tgattattta tactagttat ttagtcaatt
    19141 accccatttg gatgctttgt taccccttct gaaaaatcta taaccccgtc tgggtgtttc
    19201 aaaaccctaa ctggatggtt tatcacccta attggatact ctattacccc atgtggatgc
    19261 ttcaaaaccc taactggatg gtttatcacc ccaattagat attccattac cccatgtgga
    19321 tgcttcaaaa ccctatatag aatatacata aatgagattt acaaaaacaa gaggtttcat
    19381 ttattggtat cagaagtagc aagaaatata tacttaagca tatccgcttt caggttctaa
    19441 aagatatacc gcaaattatt caccgtttta aagtcttttt taattgcaaa attatggttt
    19501 tatgaggttt ttcaaatctg gcagacttac gtttgcaaac caaacctatg atctttcaac
    19561 ccctagtggt atagtcttgt ttctgtttcc aagactattt agcaattgac tgcattttga
    19621 attaaaccgc atctttttca tcaaaatgca tggattcata ttctagttga agaatttggc
    19681 aaagtttcat caacacagca acacttaact ctgttgttcc gttttcaatt ttgttgtagc
    19741 tgctttgggt gatgtttaat tgacttgaaa cgtagtgttg cgaatagccc aactcgtttc
    19801 ttcttttttt gattttaatt aaaattttct cgaccatata ccatagaatt aatgatttaa
    19861 attaatattt ggatttttga gacttaacta atacttgtgg tttgtgaatt tcaaaaatag
    19921 caaaaaaaaa aaatatccta caaaaaaaat gaaaatattt tattctctaa gagaataatt
    19981 ttgt
//
LOCUS       DAOOBH010000031        19934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_3589_length_19934_cov_7.027818, whole genome shotgun sequence.
ACCESSION   DAOOBH010000031 DAOOBH010000000
VERSION     DAOOBH010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 19934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19934
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3589_length_19934_cov_7.027818"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2037)
                     /locus_tag="PK218_05110"
     CDS             complement(<1..2037)
                     /locus_tag="PK218_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPW97919.1"
                     /translation="MSLAFLQTIFLRSILSFLNKKRMSFPSGQSKFRLLSLLVFVGVL
                     VSQNSFSQDTIHRKKIGLVLSGGGAKGFAHIGVLKVLENAGVKIDYIGGTSMGAVVGG
                     LYASGYNATQIDSIFEYTNFNELLQDYIPRTSKSFYEKRNDEMYAISLPFSKLKIGIP
                     IALSKGMYNYNLLAKLTHNVRNVRDFNKLPIPFVCIATDIEKGKQVILNKGYLAQAMM
                     ASSAFPSLFSPVEIDDKILIDGGVTNNYPVEELRKMGAEIIIGVDVQDDLKDRKALND
                     ATRILVQITNLDMINKMQGKVKLTDVYIKPDVSEYGVISFNDGKEIIKKGEEAALLKI
                     GEIQKFCNLQTAVQRQAVKDEVYKIDLKKIDITELKNYTRAYVLGKLRIKAGKKTTYD
                     ELKSGINTLNATQNFSRISYSIDSYQDGDALHLNLVENDTKTFLKFGLHYDDLYKSAL
                     LVNVTQKKILFKNDVLSLDMALGDNIRYNFDYYIDNGFYWSFGIKSWYSTFNRNVATD
                     FRNGEVLSQLGVDKLNIDFSDFTNQAYMQTVFIQKYLIGAGIEYKHLKIQTSTIENGD
                     SVFEDSNYFNLFGHLKYDSFDNKYFPKSGWLFYGDFKTYLYSSNYTNEFNQFSVAKGE
                     ISFAQTILKKFTIKVQSEAGFKIGNDSVDFFNFVLGGYGYNTINNFKHLYGYDYL"
     gene            complement(2053..3849)
                     /gene="uvrC"
                     /locus_tag="PK218_05115"
     CDS             complement(2053..3849)
                     /gene="uvrC"
                     /locus_tag="PK218_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPW97920.1"
                     /translation="MEQPSLELQLQTLPEGPGVYQYYDKDGKILYVGKAKNLKKRVAS
                     YFTKTHDNGKTRVLVKKIVTIKHIIVANESDAFLLENNLIKKLQPRYNINLKDDKTYP
                     WICIKKEPFSRIFPTRRMVKDGSEYFGPYTNFKIVNTLLDLIKELYPLRTCNYDLSAS
                     NIEKGKYKVCLEYHIGNCKGPCEGLETLENYQEQVHAIREILKGNFKESLRDFKSKMH
                     TFSSNMQFEEAQKIKEKIESLENYQARSTVFNPKMASVDVFSIISDETTAYVNFLQVS
                     FGAIIRSHTMEIKKKLDETNEELLELAVIELRERFKLTSKEIILPFPLDLGENIKITV
                     PQLGDKKQILDLSERNAKYQRLEQLKQMQIVDPERHTNRIMAQMKKDLRLSVEPRHIE
                     CFDNSNIQGTNPVSACVVFKDGKPSKKDYRHFNIKTVEGPNDFASMEEVVYRRYKRLL
                     DEKQPLPQLIIIDGGKGQLSSALKSLDELELRGKIAVIGIAKRLEELFYPGDSVPLYL
                     DKKSETLKIIQQLRNEAHRFGITHHRNKRSKSALVTSIETIPGIGEKTMQTLLKHFKS
                     VKRLKDATEKEISEVIGASKAKKISDFYHKIN"
     gene            4108..4584
                     /locus_tag="PK218_05120"
     CDS             4108..4584
                     /locus_tag="PK218_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97921.1"
                     /translation="MPIENLGKNHFTAAEKTQLNDAVDTIMSLIGAKTYNLSPKERSK
                     YGKIGDKMKLLITKTKEYHENTPALQSPDVNWEEFEADYETRHFASQKLLQLRAAVEM
                     MLNIKILHDHDNFQDALRDYQYAIYKNKFGNQLGFAPKIDSLKDFFPKTGKKHKKE"
     gene            4618..5589
                     /locus_tag="PK218_05125"
     CDS             4618..5589
                     /locus_tag="PK218_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HPW97922.1"
                     /translation="MPNNNELIILKETILPGESKTINMEIAKLQTMTKLKIPIIVERS
                     KKPGPTVLFSAGLHGDEINGVEIVRQLITQKINKPSCGTIICIPVLNVFGFVNQSRLF
                     PDGRDLNRVFPGSKGGSLASRFAYYILKEVIPHVDYIVDFHAGGASRFNAPQIRIVPN
                     DAELKALSEVFNAPFGLYSKNISGSFRNACTKLGKKILLFEGGKSAHIDLHITREGIE
                     GSKRFLEHLDMLSSKQKTILPKKNTIYIKKSSWIRANSSGMFHVEVKINAFVNKGQLL
                     ATISDPYGKVEHKVKAPNSGYIINVNDASIVYQGDAIFHITNTLEND"
     gene            5582..6151
                     /locus_tag="PK218_05130"
     CDS             5582..6151
                     /locus_tag="PK218_05130"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPW97923.1"
                     /translation="MTKKELRTKHKALRAKLSAEEIEEKSLQIANQLLKLDIWKNTFF
                     HIFLPIREKNEVDTEFILQILHGKDKEIVVSKSNFETLKMNHFLLTENTKLKKNEYNI
                     DEPVYGLSVPDDMIDVIFVPLLAYDKNGYRVGYGKGFYDRFLLDCRFDSIKIGLSFFE
                     PEEEFIDDVSVTDIPLDYCVTTQNVHSFR"
     gene            complement(6123..6758)
                     /locus_tag="PK218_05135"
     CDS             complement(6123..6758)
                     /locus_tag="PK218_05135"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HPW97924.1"
                     /translation="MSLRNSVLIIFLVLVIDQFSKIYVKTNFIYGEAGQIEVANWFKL
                     LLIENEGMAWGAKIPGDYGKLILTIFRIFAVGGIAYWLYDSSKKHSSNFLMVAISLIF
                     AGAVGNIIDSIFYGVVFDDSYNHLAVMFSENPYGTWFHGKVVDMFYFPIIQDYPMPEW
                     VPFIGGKPFTFFNAIFNVADVAISTGVGILIVFNKKAFTKRVSIGTNERFE"
     gene            complement(6848..7228)
                     /locus_tag="PK218_05140"
     CDS             complement(6848..7228)
                     /locus_tag="PK218_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPW97925.1"
                     /translation="MVEEKMRYSDADLAEFKEIIVKKIEKAQSDLELIKSAYMNDLNN
                     GTDDTSPTFKAFEEGSETMSKEANSQLAIRQEKFIRDLKNALFRVENKTYGICKVTGK
                     LISKERLKVVPHATMSIEAKNLQR"
     gene            complement(7236..10634)
                     /gene="ileS"
                     /locus_tag="PK218_05145"
     CDS             complement(7236..10634)
                     /gene="ileS"
                     /locus_tag="PK218_05145"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPW97926.1"
                     /translation="MSTKFTEYKGLDLPTVASEVLDFWKKNNVFEQSVTSREGATPYV
                     FFEGPPSANGLPGIHHVMARAIKDIFCRYKTQKGFQVKRKAGWDTHGLPVELGTEKEL
                     GITKEDIGKTISVEEYNEACKKTVMRYTDVWNDLTEKMGYWVDMENPYITYKSKYMET
                     VWWLLKQIYNKDLMYKGYTIQPYSPKAGTGLSSHEVNQPGSYRDVTDTTVVAQFKTKP
                     ESLPSFLQGFGDVHILAWTTTPWTLPSNTALTVGPKIDYVLVETFNQYTFRPTKVILA
                     KNLVGKQFTKGFFESTEPADFENFKEGDKKIPFQVLKEFKGTDLVGIRYEQLLPLALP
                     YQNPENAFRVISGDFVTTEDGTGIVHTAPTFGADDAKVAKEATPEVPPLLVKDANDNL
                     VPLVDLQGKFIQGLGDYSGKYVKNEYYNDGEAPERSADVEIAIQLKEENKAFKVEKYV
                     HSYPHCWRTDKPILYYPLDSWFIKITEVKDRMFDLNETINWKPKATGEGRFGNWLKNA
                     NDWNLSRSRYWGIPLPIWRNEEGTEEILIGSVEELYNEIEKSIVAGFQKENPFKGFEI
                     GNMTEENYDLVDLHKNVVDEIVLVSPSGKPMKREADLIDVWFDSGAMPYAQWHYPFEN
                     KEKIDGNLDFPADFIAEGVDQTRGWFYTLHAIGTLVFDKIAYKNVVSNGLVLDKNGQK
                     MSKRLGNAVDPFTTLAEYGPDATRWYMIANANPWDNLKFDIEGVAEVRRKFFGTLYNT
                     YSFFSLYANIDNFNYSEAEIPLNERPEIDRWILSELHTLIKLVDESYADYEPTKAARA
                     ISEFVQENLSNWYVRLCRRRFWKGEYGTDKIAAYQTLYTCLLTVAKLAAPIAPFFMDK
                     LYTDLINTTGKEDFTSVHLANFPVSVENFVDKSLESKMMKAQTVSSLVLSLRKKEMIK
                     VRQPLQKVMIPVLDDNFRAEIEAVSDLIKAEVNVKEVELLDDASGVLVKQIKPNFKTL
                     GPRFGKDMGLIAKEIQSFSTEQIKEIETNNLVVLVISGKSIILTSEDVEITSQDIPGW
                     LVANANGITVALDITINEELKEEGIARELVNRIQNLRKDSGFEVTDKIKVKIQRFGDL
                     EKAVLSNENYIKSETLTNELIFEDHLEGLEIEFDDIKTKIVISK"
     gene            complement(10756..11463)
                     /gene="recO"
                     /locus_tag="PK218_05150"
     CDS             complement(10756..11463)
                     /gene="recO"
                     /locus_tag="PK218_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPW97927.1"
                     /translation="MLVKTKAIVISSLKYQEKSLIVKCFTQSDGLKSYFVPNAFSAKK
                     SNQKIAYFQPFSILEIEANHKNKGTLEHFKEIKLAYSYQTIATDVVKSSIILFLSEML
                     HHSIKEEEENENLFTFLETALLWLDNHEEITNFHLIFLLQMTKFFGFFPNKANGNYFE
                     MIDGVFSEYQGTSCLNFQETVLLKKLLELKFDSSKKLFLASERQQLLKIIIDYYSIHL
                     DGFKKPKSLEVLKEVFA"
     gene            complement(11492..13783)
                     /locus_tag="PK218_05155"
     CDS             complement(11492..13783)
                     /locus_tag="PK218_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97928.1"
                     /translation="MKNSFYLVYIFLFQFGFSQQNLQWKGYFSYNKISDITESSSKFF
                     AATENAIFSKNLATNELISTNTIDGLSGETISAIYFSATTNKTIIGYKNGLMIVLNNQ
                     DGSILNVVDIINKSIPENIKKVNHFMEYNGILYVSCDFGIVQYNLNTLQFGDTYFIGP
                     NGLEIRVYQTTIFNNEIYAVTEYYGIKKAAISNPNLNDFAQWQIFDNGYWNGIVTFNN
                     QLIATNTNNKVYKHTGTAFQEVFNLNQTGLDTRVFENHLIVTTNNQVFVYNSNLTQAA
                     YIPSSQVSAIPLTFSCATVINDKVYIGTNENGVISAPLSNLGEFEVIMPDGPKRNYLF
                     SIHSSNSNLWAVYGGYSVTYNPYTFLNLGLTSFGVSKYTDNQWLNIPYEDVLGAKSLT
                     RISSNPNNENEVYVSSYDKGLLKIESDIPTTLFNESNSSLKSLDASSTSNIRIGPSTF
                     DRNGNLWVTNSRVSTGLNVLKTNGQWQAYNMSTIIDNVVNSDFAQIEIDNSGTKWLAT
                     YQDGVIAFNENSNIYKKITEGTDLGNLPINDVRALAIDNRSQVWIGTRKGLRVLSSTS
                     SFTSENQMKANPIIILEDGLAQELLYEQFITDIVVDGSNRKWIGTADSGAFLFSSNGQ
                     ETIYHFTKDNSPLPSNTINDIEINETTGEIYFATDKGMISLKGIATKPADDLQNVYVY
                     PNPVRPEYIGTIKIAQLVEKAAIKITDIEGNLVYETTSTGGTVEWDGTAFGSYKVASG
                     VYMIFISSEDGSDTTVKKVMIIR"
     gene            complement(13912..15255)
                     /gene="gdhA"
                     /locus_tag="PK218_05160"
     CDS             complement(13912..15255)
                     /gene="gdhA"
                     /locus_tag="PK218_05160"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPW97929.1"
                     /translation="MSQSINAFIEAVAKKNPNEPEFMQAVKEVAETVIPFIEENKKYQ
                     NKMLLERMVEPERVIMFRVTWLDDAGKTQVNRGYRIQMNSAIGPYKGGIRFHPSVNLS
                     ILKFLAFEQTFKNSLTTLPMGGGKGGSDFDPKGKSDNEIMKFCQSFMTELSRHIGADT
                     DIPAGDIGVGGREVGYMFGQYKRLRNEFTGVLTGKGISFGGSLIRPEATGYGDVYFAQ
                     NMLATKGDSFKGKTVVVSGSGNVAQYAIQKATQLGGKVVTASDSSGYIHDTDGIDADK
                     LAHIMEIKNVNYGRIGDYVKKYPNAKFVAGKRPWEVKCDIALPCATQNELNEEEAKML
                     VKNGCICVAEGANMPSTPEAVEVFLKAKILFAPGKASNAGGVATSGLEMSQNSLRLSW
                     SSEEVDQKLHGIMENIHASCVKYGADGSGFVDYVKGANIAGFVKVADAMLAQGIV"
     gene            15505..16296
                     /locus_tag="PK218_05165"
     CDS             15505..16296
                     /locus_tag="PK218_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HPW97930.1"
                     /translation="MQKFIKIIFTFILLIGMCKSTQAQFGFSHELGVIAGPVAFQSDY
                     GERHDFSTNSGNTGYGIGIIHYLNFSYKAECNCYTPETYFNDHFKLRSELSYNKTNLK
                     HYGQWVDPSKTSLIANQLRGMRGSTAVTNIGMQLEFYPLSIRDFTATLGSFAPFVSLG
                     GQFSFYDPEAYSTLGPITSPLAIPDKYLGGAITNQGGTVWSIVSSIGSRYKLTELSDL
                     MIDLRVQYYFSNWVDGLNPDPKRYPENKANDWNVWLNFGYIYYLN"
     gene            complement(16293..17513)
                     /gene="hflX"
                     /locus_tag="PK218_05170"
     CDS             complement(16293..17513)
                     /gene="hflX"
                     /locus_tag="PK218_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPW97931.1"
                     /translation="MLEKNSSNFEKTIVVGIVTQNQSEEKLQEYLDELEFLTFTAGGE
                     VIKRFHQKLEKPNPKTFLGTGKIEEINLFVKNNAVSTVIFDDELSPSQQKNLSKIIDC
                     KILDRTNLILDIFAQRAETSYARTQVELAQCQYLLPRLSGLWTHLERQKGGIGLRGPG
                     ETEIETDRRIVRDRIALLKEKIKTIDKQMAVQRSNRGAMVRVALVGYTNVGKSTLMNA
                     IGKSDVFVENKLFATLDTTVRKVVIKNLPFLLSDTVGFIRKLPTQLIESFKSTLDEVR
                     EADLLLHVVDISHQDFEDHIQSVNQILMDIKSNNKPTIMVFNKIDAFKNLSIDSDDLI
                     TEKTTKHYTLEEWKKTWMNNVGEKNTLFISATHKENFEELREKIYEAVREIHITRFPY
                     NKFLYPDYKDAVED"
     gene            17672..>19934
                     /locus_tag="PK218_05175"
     CDS             17672..>19934
                     /locus_tag="PK218_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPW97932.1"
                     /translation="MKKITLLLLTLLFSFSGFSQFGGTGVVEGFESTTGPSALPSNNW
                     ALSTGNWLVFDNGTPGNFRWGISTVTTTPTQVYQGANAAYMNRETNFSTGQTYEDYLA
                     SPAFTVPANGQLRFWTRGFLIGNQGTVYQIKVAPAAANPTDPNSYLLVQQWTDADLVA
                     SVSQYEEKVVNLTDYVDLNVRIAFVMQQAPITAPFGDRWFVDNVQVVAQCFDPTALTA
                     TPAITSAQLSWTNPGTTSWEVELVPAAGTPTGIGTPFNGAVPSFNVTTTLPAGVPLTA
                     TTAYKYYVRAVCSVGVTSQWIGPFNFSTLSPGLSCEASIVINNLPYSTTDSTANYGDN
                     PNIEGSPGTGCNITGNYLNGNDVVYSYTAPANGTINVTMTPTGTNSGVFAYDSCANVG
                     VTCIGGAAGTTTTVRTFDLSVTAGQTYYFVISSINTTQTVGYTLLIQNVNCAPPTALA
                     ANALQTTANLSWAQGGSSSWEVVVQPAGAGVPAGAGTQTNINTNYPVPTVLTPNTNYE
                     FYVRADCNDGTGNFSAWAGPFAFRTLCDAFTVPFQEGFNSTSTSEACWTVLNVNGDTD
                     AWDMNYATNPFEGDQVAMMYTDFNAGVNDDWLISPQIILTGNQRLRYRYRVQSAGEPN
                     DFRVMLSTNGTSPANFTTTLVPLASYNNITYMEAIVNLSAYSGPVNIAWHVPPGGLDG
                     WRLYIDNVIIEDLPTCPEPSMLTATAVLSTSATLTWTNGNAETEWQVLALPCGSPAPA
                     AGATGFVTVPPTGS"
BASE COUNT         6248 a         3489 c         3220 g         6977 t
ORIGIN      
        1 taggtagtca tagccataaa gatgtttgaa attatttatg gtattatagc catatccacc
       61 gagaacaaaa ttaaaaaaat caacactatc attccctatt ttaaatcctg cttcggattg
      121 tacttttata gtgaattttt ttaatatagt ttgtgcaaaa cttatttcac ctttggcaac
      181 agaaaactga ttgaactcat tagtatagtt tgacgaatac aaataggttt tgaaatcacc
      241 ataaaacaac caacctgatt ttgggaaata tttgttgtca aaggaatcat attttaaatg
      301 accaaaaaga ttaaaataat tactgtcttc aaaaacagaa tcgccatttt ctattgtgga
      361 agtctgaatt tttaaatgtt tatattctat tcctgcacct attagatatt tttgaataaa
      421 tacagtttgc atataagctt gattggtaaa atctgaaaaa tcgatgttca gtttgtcaac
      481 gcccaattga cttaagactt caccatttct aaaatcagtt gcaacatttc tattgaaagt
      541 gctgtaccaa gacttgattc caaaactcca ataaaaacca ttgtcaatgt aataatcaaa
      601 attatatctg atattgtcac ccaacgccat atctaaggat aaaacatcgt ttttgaatag
      661 tatttttttc tgtgtcacat tgactaataa tgcgctttta tacaaatcgt cataatgtaa
      721 accaaacttt aaaaaagttt tggtgtcatt ttctactaag tttaaatgaa gtgcatctcc
      781 atcttgatat gaatcgatac tataactaat tctgctaaaa ttttgagttg catttaatgt
      841 atttataccg ctttttagtt catcatacgt tgttttttta cccgctttta ttctaagttt
      901 tcccaaaaca taggctcttg tataattttt taattcggta atgtcaattt tctttaggtc
      961 aattttataa acctcatctt taactgcttg tctttgtact gctgtttgta aattacaaaa
     1021 tttttgaatt tctccaattt ttaaaagtgc ggcttcctct ccttttttaa taatttcttt
     1081 tccgtcatta aaagaaatga caccatattc agaaacatca ggttttatgt aaacatcagt
     1141 tagtttaact tttccttgca ttttattaat catgtctaaa ttggtaattt gaaccaatat
     1201 tcgagtggca tcatttaatg cttttcgatc ttttaaatca tcttgaacat caacaccaat
     1261 aatgatttca gcacccatct ttcgcaattc ttctactgga taattattgg taacaccgcc
     1321 atcaattagt attttatcat ctatttcaac gggtgaaaat aaagaaggaa atgccgaact
     1381 tgccatcata gcttgtgcca agtacccttt gtttaaaatc acttgttttc ctttttcaat
     1441 atctgttgca atgcaaacaa atggaattgg tagtttatta aaatcgcgaa cgtttctaac
     1501 attatgagta agttttgcca ataaattgta attgtacatt cctttagata atgcaatagg
     1561 gattcctatt tttagtttac taaaaggcaa cgaaatggca tacatttcat cattcctttt
     1621 ttcataaaag cttttagacg ttcttggaat ataatcttga agcagttcgt taaaatttgt
     1681 atattcaaag atggagtcaa tttgtgttgc attgtaaccc gaagcataca atccgccaac
     1741 aactgcaccc atactggttc cgccaatata atctattttt acaccagcat tttctaaaac
     1801 ttttaatact cctatgtgag caaatccctt ggcaccacca cccgaaagca ctaaacctat
     1861 tttttttcta tgtatagtgt cctgtgaaaa ggagttttga gaaacaagta caccaacaaa
     1921 aaccaataga ctcaaaagac gaaacttact ttgccctgat ggaaacgaca tccttttttt
     1981 gtttaaaaaa gagagaatag accgcaggaa aatggtttgt aaaaatgcta aagacattcg
     2041 cttcaaattg gtttaattga ttttatggta aaagtcggaa atttttttgg cttttgaagc
     2101 accaatcact tccgaaattt ctttttcagt agcatccttc aatcttttaa cacttttaaa
     2161 atgttttagt aacgtttgca tcgtcttttc accaatccca ggaatggttt ctatgctcgt
     2221 cactaacgct gatttgctgc gtttattgcg gtgatgggtg atgccaaatc ggtgggcttc
     2281 gtttctaagt tgttggatga tttttaatgt ctccgatttt ttatctaaat acaacggtac
     2341 cgaatcgccg ggataaaaaa gttcttctag tcgttttgca atgccaatga cagcaatttt
     2401 tccgcgcaat tctaattcgt ccaaactttt taaagccgac gaaagttgtc cttttccgcc
     2461 atcaatgatg atgagttgtg gcaagggttg cttttcgtct aataatcgtt tgtagcgacg
     2521 gtacaccact tcttccattg aagcaaaatc atttggtcct tcaacggttt tgatgttaaa
     2581 atgacggtag tcttttttgc tcggttttcc atctttaaaa actacgcaag ccgatacagg
     2641 attggtgcct tgaatattgg aattgtcaaa acattcaata tgtcgcggct ctaccgatag
     2701 gcgtaaatct ttcttcatct gcgccataat tcggttggtg tgtcgctctg ggtcaacaat
     2761 ttgcatttgt tttagttgct ccagtctttg gtatttggca ttgcgctcgg ataaatcgag
     2821 gatttgtttt ttgtcgccca gttgtggtac tgttattttg atgttttcgc ccaaatcgag
     2881 tggaaaagga agaattattt ctttggaagt taatttaaat cgttctcgga gttcaattac
     2941 ggcaagttca agcaattctt cgttggtttc gtctaatttt ttcttgattt ccatggtatg
     3001 cgaacgaatt attgcgccaa aagaaacttg caggaaatta acatacgccg tggtttcgtc
     3061 cgaaatgatg gaaaacacat ctaccgaagc catttttggg ttaaaaaccg tagaacgtgc
     3121 ctgataattt tcgagcgatt caattttttc tttaattttt tgcgcttctt caaattgcat
     3181 gttgctcgaa aaagtgtgca tttttgactt aaaatcgcgc aaactttctt taaaattacc
     3241 tttcaatatt tcacgaatag catgcacttg ttcttgatag ttttctagtg tttccagtcc
     3301 ttcacacggt cctttgcagt tgccaatgtg gtattctaaa cacactttgt actttccttt
     3361 ttcgatgttg gaagcgctta aatcataatt acatgttcgc aatgggtaca actccttaat
     3421 caaatcgagt agtgtattga cgattttaaa attggtatag ggaccaaaat attccgagcc
     3481 gtctttaacc attcttcgcg ttggaaatat cctcgaaaaa ggttcttttt tgatgcaaat
     3541 ccacggatag gttttgtcgt cttttaggtt gatattgtag cgtggttgta gttttttgat
     3601 gaggttattt tcaagcaaaa aagcatccga ttcgttggct actatgatgt gttttatggt
     3661 cacaattttt ttgaccagca cgcgcgtctt accgttgtcg tgggtttttg taaaatagga
     3721 agccactctt tttttgaggt ttttggcttt gcctacatac aatattttgc cgtccttatc
     3781 ataatattga taaacaccag gaccttcggg caaggtttgc aattgaagtt cgagtgacgg
     3841 ttgttccata aacgagtaca catttgtgtt acaaaaataa gataaaatta tggactagca
     3901 gaatgcctct tcaatggggt tatgaaccac ttaaattaat aaatttcatt aattaattat
     3961 taaattttat caattaataa ttatatatca tttaattatt aatgatattt atttaaaatt
     4021 aaatattttt cataaatatt ttaataaagt ttatttaaaa tttaataaaa aacattaatt
     4081 ttatatttaa attaatctgt tattagtatg cccatagaaa atttaggaaa aaatcatttt
     4141 acagctgccg aaaaaacgca gttaaatgat gcagtcgata ccattatgag tcttattggt
     4201 gctaaaacct ataatttgag cccaaaagaa cgtagcaaat atggcaaaat tggcgacaag
     4261 atgaagctgc tgattaccaa aaccaaagag tatcacgaaa acacaccggc gctgcaatcg
     4321 cccgatgtga attgggaaga atttgaagcc gactatgaaa ccaggcattt tgcgtcgcaa
     4381 aaattgttgc aacttcgagc ggcagtagaa atgatgttga acatcaaaat tttgcacgat
     4441 cacgataatt ttcaagatgc tttgcgcgat tatcagtatg ctatttataa aaataaattt
     4501 ggcaatcaat tgggttttgc gcccaaaata gattccctaa aagatttttt tccaaagacc
     4561 ggaaagaaac acaaaaaaga ataattgatt tatttttcat ccaaaacata ccacttcatg
     4621 cccaacaata acgaactaat tatactcaaa gaaaccattt tgccaggcga aagcaaaacc
     4681 atcaacatgg aaattgcgaa gttgcaaacg atgaccaaac tgaaaattcc aatcattgta
     4741 gaacgttcta aaaaacctgg tccaacggtg cttttttcgg cgggtttgca tggcgatgaa
     4801 attaatggtg ttgaaattgt gcgccaactc atcacccaaa aaattaataa accaagttgt
     4861 ggcacaataa tttgcattcc ggttttgaat gtttttgggt ttgtcaatca atcacgtctg
     4921 tttccggatg gaagagattt gaaccgtgtt tttccgggca gcaaaggcgg ttccttggca
     4981 agcaggtttg cctattatat tttaaaagaa gtgattccac atgtagatta tattgtcgat
     5041 tttcatgctg gcggtgctag tcgatttaat gcaccacaaa ttagaattgt gcccaatgat
     5101 gcggaattaa aagcactttc agaagttttt aatgcgccat ttgggttgta ttctaaaaac
     5161 atttcgggta gttttagaaa cgcttgtacc aaattgggta aaaaaatatt gctttttgaa
     5221 ggcggaaaat cggcacacat cgatttgcat attactagag aaggcattga aggttcgaaa
     5281 cgctttttag aacatttgga catgttatcg tccaaacaaa aaaccattct gcccaaaaaa
     5341 aatacgattt acatcaaaaa atcaagctgg attagagcca actcgtcggg aatgtttcat
     5401 gtggaagtaa aaattaatgc ttttgtgaat aaaggacaat tgttggctac tatttctgat
     5461 ccttatggaa aagttgaaca caaagtaaaa gcacccaatt cgggttatat aatcaatgta
     5521 aacgacgctt caattgtata tcaaggcgat gcaatatttc atattacaaa tactttagaa
     5581 aatgactaaa aaagaattac gaacaaaaca caaagcacta cgggctaaac tttcggcgga
     5641 agaaattgag gaaaaaagtt tgcagatagc taatcaatta ttgaaattag acatttggaa
     5701 gaatactttt tttcatattt ttttgccaat cagagaaaaa aacgaagtcg atactgagtt
     5761 tatactgcaa attcttcatg ggaaagacaa ggaaattgtg gtttcaaaaa gcaattttga
     5821 aacgctgaaa atgaatcatt ttttattaac agaaaacaca aaacttaaga aaaacgaata
     5881 taatattgat gaacccgttt atggtttgag tgttcctgat gatatgattg atgtgatttt
     5941 tgtaccgcta ttggcgtatg ataaaaatgg ttatagagta ggatatggca aaggatttta
     6001 tgatcgcttt ttgttagatt gccgattcga ttctattaaa atagggcttt cgttttttga
     6061 acctgaagaa gaatttatcg atgatgtttc ggttactgat attccgctag attattgtgt
     6121 aactactcaa aacgttcatt cgttccgata gaaactcttt tagtaaatgc ttttttgttg
     6181 aagacaatca atattccaac accagtagaa attgccacat cggccacgtt aaaaatagca
     6241 ttgaaaaaag taaatggttt tccaccaata aacggaaccc attcgggcat tggataatct
     6301 tgtataattg ggaaataaaa catgtcaact acttttccgt gaaaccatgt accataaggg
     6361 ttttcagaga acatcactgc caaatgattg tagctgtcgt caaaaacaac gccgtaaaaa
     6421 atagaatcaa taatatttcc aacagcacca gcaaaaatta atgaaatggc gaccatcaaa
     6481 aaattagagc tgtgtttttt gctagaatca tacaaccaat aagcaatgcc gccaactgca
     6541 aaaattctaa aaatggttaa aattaatttt ccataatcac ccgggatttt tgcaccccaa
     6601 gccattcctt cgttttcaat caacaatagt ttgaaccaat tggcaacttc tatttgtccg
     6661 gcttcaccat atatgaaatt agttttgaca taaattttag agaattgatc aatcactaat
     6721 actaggaaaa tgattaaaac agaattgcgt aaagacattt tttgaaaatt aatggtgcaa
     6781 aaatagtgtt tttttataaa aaataacgct cctatgagga gcgttaaatg attggttttt
     6841 ttattattta tcgctgtaaa ttctttgcct caatgctcat ggtagcatga ggaacaactt
     6901 ttaagcgttc tttgcttata agttttccgg ttactttgca aatgccataa gttttgtttt
     6961 ctacacggaa aagtgcattt ttcaaatcac gaatgaattt ttcttgacga atagctaatt
     7021 gtgaatttgc ttctttagac attgtttcac taccttcttc aaatgcttta aaagtaggcg
     7081 atgtatcatc agtaccgtta tttaaatcat tcatataagc actttttatc aattctaaat
     7141 cggattgtgc tttttctatt ttttttacaa taatttcttt gaactcagct aaatcagcat
     7201 ctgagtatct cattttttct tctaccatct tttttctatt ttgagattac tattttggtt
     7261 tttatatcat caaattcaat ttctaaaccc tctaaatggt cttcaaaaat caattcattt
     7321 gttaatgttt ccgatttaat ataattttca ttagaaagga ctgctttctc taaatcgcca
     7381 aatcgttgaa ttttcacttt tattttatca gtaacttcaa atcctgagtc ttttctaaga
     7441 ttttgaattc tgtttaccaa ttctcttgca ataccttctt cttttaattc ttcgttgatg
     7501 gtaatatcta atgcaacagt tataccattg gcattggcga ccaaccaacc tggaatatct
     7561 tgagaagtaa tctcaacgtc ttcggatgtt aaaattatac tttttcctga aataacaagt
     7621 actaccaagt tattggtttc aatttcttta atttgctcag tgctgaagct ttgtatttct
     7681 ttggcaatca aacccatatc tttgccaaat cgtgggccaa gtgtcttgaa attgggttta
     7741 atttgcttta ctaatacacc tgatgcatcg tctaaaagct cgacttcttt aacatttact
     7801 tctgctttta tcaagtcgga aactgcttca atttcagctc taaaattatc gtcaagcact
     7861 ggaatcatta ccttttgcaa cggttgacgc accttaatca tttccttttt tcgaagtgat
     7921 aaaacgagtg acgaaactgt ttgtgctttc atcattttgc tttccaacga tttatcaaca
     7981 aagttttcaa ccgaaactgg gaaatttgct aagtgaacgc tggtaaaatc ttcttttcca
     8041 gttgtgttaa tcaaatcagt atataatttg tccataaaaa atggcgctat tggagctgct
     8101 aatttggcaa cggtcaacaa acaagtatat aacgtttgat atgctgcaat tttatccgtt
     8161 ccatattcgc ctttccagaa tcttcttcta caaagtcgaa cataccagtt gcttaaattt
     8221 tcttgaacaa attctgaaat tgctcttgct gcttttgttg gttcataatc agcatacgat
     8281 tcatctacta attttatcaa agtatgtaat tctgataaaa tccatcggtc aatttctggg
     8341 cgttcattca acggaatttc agcttcagaa taattgaaat tatcaatatt ggcatacaaa
     8401 ctaaagaacg aatacgtgtt gtataatgtt ccaaagaatt tacgacgaac ctcagcaaca
     8461 ccttcaatat caaattttaa attgtcccaa ggatttgcat tggcaatcat gtaccaacgt
     8521 gtagcatctg gaccatattc tgccaatgtt gtaaatggat caacagcatt tcctaaacgt
     8581 ttagacattt tttgtccgtt tttgtctaga actaatccgt ttgaaactac atttttataa
     8641 gcaattttat caaaaactaa tgttccaata gcgtgtaacg tataaaacca accacgtgtt
     8701 tggtcaacac cttctgcaat aaaatcagct gggaaatcta aatttccatc aattttttct
     8761 ttgttttcaa acggataatg ccattgtgca taaggcattg cacccgaatc aaaccaaaca
     8821 tcaattaaat cggcttcacg tttcattggt ttacctgacg gcgaaaccaa tacaatttca
     8881 tcgactacat ttttatgtaa atcaactaaa tcgtaatttt cttcagtcat atttccaatt
     8941 tcgaatcctt taaatggatt ttctttttgg aaaccagcaa cgattgattt ttctatttcg
     9001 ttgtataatt cttcaacaga accaattaaa atttcttctg ttccttcttc gtttctccat
     9061 attggtaaag gaatacccca atatcttgaa cgtgataaat tccagtcatt cgcatttttt
     9121 agccaatttc caaaacgtcc ttcaccagta gcttttggct tccagttaat cgtttcattt
     9181 aaatcaaaca ttctatcctt aacttcagta atttttatga accaagaatc taatggataa
     9241 tataaaatag gtttgtctgt tcgccaacaa tgtggataac tgtgtacata tttttcaact
     9301 ttaaaagcct tattttcttc ttttaattgg atggcaattt caacatcggc agaacgctct
     9361 ggagcttcac catcgttgta atattcgttt ttaacatatt tcccagaata atcgccaaga
     9421 ccttgaataa atttgccttg taaatctact aatggaacta aattatcatt ggcatcttta
     9481 accaataatg gtggaacttc tggcgttgct tctttagcta cttttgcatc atcagcacca
     9541 aatgttggcg cagtgtgaac aattcctgtt ccatcttcgg tagtaacgaa atcgcctgaa
     9601 attactctaa aagcattttc tggattttga tatggtaatg ctaatggtaa taattgttcg
     9661 taacgaattc cgactaaatc agtgccttta aattctttta gaacttgaaa aggaattttt
     9721 ttatcacctt ctttaaagtt ttcaaaatca gctggctctg tactttcaaa aaaaccttta
     9781 gtaaattgtt ttccaactaa atttttagct aaaatcactt ttgtaggtcg aaaagtatat
     9841 tgattaaatg tttccactaa aacataatcg atttttggtc caacagtcaa cgcagtattt
     9901 gaaggtaatg tccaaggcgt tgtggtccaa gctaaaatat gaacatcgcc aaaaccttgt
     9961 aaaaaacttg gtaaagattc tggttttgtt ttaaattgag ctacaactgt tgtatcggta
    10021 acatcgcgat aacttcctgg ttggtttact tcgtgagaag ataatcctgt tccagctttt
    10081 ggagaatacg gttgaatggt gtaacctttg tacatcaaat ctttgttata gatttgtttt
    10141 aaaagccacc aaaccgtttc catgtatttt gatttataag taatgtatgg attttccata
    10201 tctacccaat accccatttt ttcggtcaaa tcgttccaaa catctgtgta acgcatgacg
    10261 gtttttttac acgcttcgtt gtattcttct accgaaatgg ttttgccaat atcttcttta
    10321 gtaattccta attctttttc agtgcctaat tctacgggca atccgtgtgt atcccaacct
    10381 gcttttcgct ttacttggaa acctttttga gttttatagc gacaaaaaat atctttaatt
    10441 gctcttgcca tcacatggtg aattccaggc aaaccatttg cagatggtgg cccttcaaaa
    10501 aacacataag gcgttgcacc ttcacgtgaa gttacacttt gttcgaagac attatttttt
    10561 ttccagaaat caagaacttc tgaagccaca gtaggcaagt caagtccttt gtattcagta
    10621 aacttagtac tcatttttgt cgttttcttt aatcagtttg cgaaagtaag gaattttaag
    10681 aaaagagaat agaaaataga tgaaagacaa cagactttta agaaaatttt aaaatttaaa
    10741 taacttttca actaattatg caaaaacttc ttttaaaact tccaaggatt taggtttttt
    10801 gaaaccatcg agatgaattg aatagtaatc gattatgatt tttagtaatt gttgacgttc
    10861 tgaagctaaa aaaagttttt ttgaactgtc gaatttgagt tctaaaagct tttttaatag
    10921 tacggtttct tgaaaattaa gacaacttgt tccttgatat tctgaaaaaa caccatcaat
    10981 catttcaaaa taatttccat tggctttatt tgggaagaaa ccaaaaaact tggtcatttg
    11041 aagaagaaaa attaaatgaa aattagtaat ttcttcatga ttatcgagcc aaagcagtgc
    11101 cgtttcaaga aacgtaaaga ggttttcgtt ttcttcctct tctttgatgc tgtgatgcaa
    11161 catttccgaa agaaataaaa ttattgaact tttcacaacg tcagttgcaa tggtttgata
    11221 agaataggct aactttattt ctttaaaatg ttctaaggtg cctttattct tgtgattggc
    11281 ttcgatttct agaatagaaa aaggttgaaa ataggctatt ttttgatttg attttttagc
    11341 cgaaaatgca tttggaacaa aatagctttt tagtccatca ctttgagtaa aacatttaac
    11401 tatcaatgat ttttcttgat attttagaga agaaataaca atagctttgg tttttaccaa
    11461 cattgggatt tggaattttt ccgtttggaa tttatcgaat tatcatcact ttttttacgg
    11521 ttgtatcaga accatcctct gaggaaataa aaatcatata aacaccagaa gctactttgt
    11581 aactgccaaa agcagtacca tcccactcca ctgttccacc agttgatgtg gtctcataaa
    11641 ctaaatttcc ttctatatcg gtgattttaa tggccgcttt ttcaaccaat tgagcaattt
    11701 tgatggtgcc aatatattct ggtcgaactg gattaggata aacatatacg ttttgtaaat
    11761 catccgcagg ttttgtagcg attcctttta aggaaatcat ccctttatcg gttgcaaagt
    11821 aaatttcacc ggtagtttcg ttaatttcta tgtcgtttat ggtattactt ggcaaaggag
    11881 aattgtcttt agtaaaatga taaatggttt cttgaccatt tgaagaaaaa agaaatgctc
    11941 cagaatcggc agttccaatc cattttctat tagagccatc gactacaata tcggtaatga
    12001 attgttcata aagaagttct tgcgctaatc catcttcaag aatgattatt ggattggctt
    12061 tcatttgatt ttcactagta aaactgctag tgctcgaaag aacacgaagc ccttttctgg
    12121 ttccaatcca aacttgactt ctattgtcaa ttgccaaagc tctaacatca tttattggca
    12181 agtttcctaa atctgtgcct tctgtaattt ttttataaat attagaattc tcattaaaag
    12241 cgataacacc atcttggtag gtagccaacc attttgtacc gctattgtca atttctattt
    12301 gagcaaaatc actgttgaca acattgtcta ttatggttga catattgtaa gcttgccatt
    12361 gtccgtttgt ttttaatacg tttagaccag tactaacacg gctattcgtt acccacagat
    12421 ttccgtttct atcaaatgtt gaaggtccaa ttcggatgtt acttgtagaa cttgcatcta
    12481 gggattttaa agaactattg ctttcattaa aaagtgtagt gggaatatca ctttcaattt
    12541 tcaataaccc tttatcataa gaacttacgt aaacttcatt ttcattgtta gggtttgaag
    12601 aaattcgtgt tagtgatttt gctcctaaaa catcttcgta cggaatattc aaccattgat
    12661 tatcggtata tttactaact ccgaacgagg tcaagccaag atttaaaaaa gtatatggat
    12721 tataggtaac actatatcca ccataaacag cccaaagatt tgaattggaa gaatgaatag
    12781 aaaatagata atttcttttt ggcccatcgg gcatgatgac ttcaaattca cctaaattgc
    12841 ttagtggtgc cgaaataaca ccgttttcat tggtgccaat atatacttta tcattaataa
    12901 cggttgcgca actaaatgtt aaaggaatgg cagaaacttg cgaacttggt atgtatgctg
    12961 cttgggttaa gttagaattg taaacaaaaa cctgattgtt tgtcgttaca attaaatgat
    13021 tttcaaaaac cctagtgtct aaccctgttt gatttaaatt aaatacttct tgaaaggcag
    13081 ttccagtatg tttgtaaact ttattattag tgttggtagc aattaactga ttgttaaaag
    13141 ttacaattcc gttccagtat ccattatcaa aaatttgcca ttgcgcaaaa tcattcaaat
    13201 ttggattaga aatagctgcc ttttttatac cataatattc cgttacggca taaatttcat
    13261 tattaaaaat agtagtttga tagactctaa tttctaaacc gtttggtcca atgaaataag
    13321 tatctccaaa ttgtaaggtg ttcaaattgt attgaacaat tccgaagtca catgaaacat
    13381 atagtattcc gttatattcc ataaaatgat tgactttttt aatgttttcg ggaatagact
    13441 tattgatgat gtcaactaca tttaaaatgc ttccgtcttg gttgtttaaa acaatcatca
    13501 aaccgttttt atacccaata attgttttgt tagttgtcgc actaaaatag attgcggaaa
    13561 tagtttctcc agataatcca tctatagtat tagtagaaat taattcattt gttgctagat
    13621 ttttggaaaa tatagcattt tcggttgcgg caaaaaattt tgaggaagat tctgttatat
    13681 cactaatttt attgtatgaa aaataacctt tccattgcag gttttgttgt gaaaaaccaa
    13741 attgaaataa aaatatataa accaagtaaa atgaattttt catagcgtca attagaatta
    13801 caaatatagt ataaacgaaa aacttttact tttaatataa aaagaataaa aaaaaatgct
    13861 tccatttctg aaagcatttt tgatttttgt tattttataa ctatattata tttatacaat
    13921 tccttgtgct agcatagcat ctgccacttt aacaaaccca gcaatgttgg ctcctttaac
    13981 ataatcaaca aaaccacttc catcagcacc atattttaca catgatgcat gaatattttc
    14041 catgattcca tgtaattttt gatccacttc ttctgacgac cagcttaaac gaagtgagtt
    14101 ttgagacatt tcaagcccag aagttgcaac accaccagca tttgaagctt ttcctggcgc
    14161 aaataatatt ttagctttta aaaatacttc tacggcttct ggagtagaag gcatattggc
    14221 tccttcagct acacaaatac atccgttttt aaccaacatt ttagcttctt cttcgttcaa
    14281 ttcgttttga gtagcacatg gcaatgcgat atcacactta acttcccacg gacgctttcc
    14341 tgccacaaat tttgcgtttg gatatttttt tacataatca ccaattctac catagtttac
    14401 atttttgatt tccatgatat gagctaattt atctgcatct attccatctg tatcatgaat
    14461 atatcctgat gaatcggaag cggtaactac ttttcctcct aattgggttg ctttttgaat
    14521 tgcatattgc gctacatttc ctgatccaga aaccactaca gtttttcctt taaaagaatc
    14581 tcctttggta gccaacatat tttgagcaaa ataaacatca ccataacccg tggcttctgg
    14641 acgaattaac gaacctccaa aagagatgcc ttttccagtt aaaacacctg taaattcatt
    14701 tctcaatctt ttatattgac caaacatata accaacctct cttcctccaa cacctatatc
    14761 tcctgctgga atgtcggtat ctgcaccaat atgtcttgaa agttccgtca taaaactttg
    14821 acagaacttc ataatttcat tgtctgattt cccttttgga tcaaaatcgg agcctccttt
    14881 tccaccaccc attggaagtg ttgttaaact gtttttaaac gtttgctcaa aagctaaaaa
    14941 ttttaaaata ctaagattta ctgaaggatg aaaacgaatt cctcctttgt agggtccaat
    15001 tgcagaattc atttggattc tgtaaccacg gtttacttga gttttaccag catcgtctaa
    15061 ccaagtaact ctaaacatga ttactctttc tggttcaacc attcgttcca aaagcatttt
    15121 gttttggtat tttttatttt cttcaataaa aggaataact gtttcggcta cttctttaac
    15181 cgcctgcatg aactcaggtt cattagggtt ttttttagcc actgcttcaa taaaagcatt
    15241 aatactttgc gacataatta ttttttttta aattttaaga aatcgatttc gtaatgatgc
    15301 gcaaatatac attttattaa aatagtgctc tatttttttt aaaaatattc ttttttcaag
    15361 taaaaaaaat aactctgaaa accaataatg tcttgataca atcttttttt tacccttttt
    15421 taaagttaaa aatcaactat tttgtatttt tttttataac tgaatgatgt ttttatatat
    15481 ttgtcgggaa tagaattata aagtatgcaa aagttcatca aaattatttt cacatttatc
    15541 ttattgatag gtatgtgtaa aagtactcaa gctcaatttg gcttctctca cgaattaggt
    15601 gtaattgctg gaccagtagc atttcaatca gattatggtg aaagacatga ttttagcaca
    15661 aattctggaa atactggtta tggtattgga ataattcatt acttgaattt ttcttataaa
    15721 gcagaatgta attgttatac tcctgaaact tatttcaacg accattttaa attaagaagt
    15781 gagttatctt ataataaaac caatttaaaa cactatgggc aatgggttga ccctagcaaa
    15841 acttcattaa ttgccaatca attgagaggt atgcgtggaa gtacagcggt tactaatatt
    15901 ggtatgcaat tagaatttta ccctttaagt attagagatt ttaccgcaac attaggctca
    15961 tttgctcctt ttgtgagttt aggagggcaa tttagttttt atgatcccga agcttattct
    16021 actcttggac caattacttc tccactcgct attcctgata aatatttagg tggtgctata
    16081 accaatcaag gtggaacggt ttggtcaatt gtttcttcta ttggttcaag atacaagtta
    16141 acagaactat ctgacttaat gattgattta cgtgttcaat attatttttc aaattgggta
    16201 gatggtttaa atccagatcc taaaagatac ccagaaaaca aagcaaatga ttggaatgtt
    16261 tggttgaatt ttggatacat ctattattta aattaatctt caacggcatc tttataatca
    16321 gggtatagga atttattata aggaaacctt gtgatatgaa tttctctaac ggcttcataa
    16381 attttttctc tcaattcttc aaagttttct ttgtgagttg ccgaaatgaa taaggtgttt
    16441 ttttcgccca cattattcat ccaagttttt ttccattctt ctaaggtgta atgttttgtg
    16501 gttttttcag taattaaatc atcactgtca atagaaaggt ttttaaaagc atctattttg
    16561 ttaaaaacca ttatagtagg cttgttatta cttttaatat ccatcaatat ttgatttaca
    16621 gattgaatat ggtcttcaaa atcttgatgc gaaatatcaa ccacatgcaa aagcaaatcg
    16681 gcttctcgaa cttcatccag agtacttttg aaagattcaa ttaattgagt tggtaatttt
    16741 ctaataaaac caaccgtgtc tgacaataaa aaaggtaggt ttttaataac cacttttcta
    16801 acagtagtat caagggttgc aaataattta ttttctacaa agacatcgct tttcccaata
    16861 gcattcatca aggttgattt tccaacgttg gtatatccaa ccaaggcaac tctaaccatg
    16921 gcaccacggt tacttctttg aaccgccatt tgtttgtcaa tagttttaat tttttccttt
    16981 aacaatgcaa ttctatcacg aacaatcctt ctgtccgttt caatttctgt ttctccaggt
    17041 ccacgaagtc caattccacc tttctgccgc tcaagatgtg tccaaagacc cgataatctt
    17101 ggaagtaaat attgacattg ggctaattcg acttgtgttc ttgcatacga cgtttcagct
    17161 ctttgagcaa aaatatcaag aatcaaattt gttctatcta gaattttaca atcaattatt
    17221 ttggaaagat ttttttgttg agatggtgat aattcatcat caaaaataac tgttgaaaca
    17281 gcattatttt tgacaaaaag attgatttct tcaatttttc ctgttcccaa aaaagtcttt
    17341 ggattgggtt tttctaactt ttgatgaaat cttttaatca cttctcctcc tgccgtaaaa
    17401 gttaagaact ctaattcgtc caaatattct tgtaattttt cttcagattg attttgagtg
    17461 acgattccaa cgacaattgt tttttcgaaa tttgaactgt ttttttctaa cattattgat
    17521 ttttagtact gcaaaattag aaataataat atttaacact cattttatag ttgtttttta
    17581 atttaaattt ttaaaaaagt tatttttttc ataaaatttt ctaaatttgg aactcttaac
    17641 ccaatattaa ttattacaac cattttcatt tatgaaaaaa attactttat tattgttgac
    17701 tttactgttt tctttctcgg gcttttctca atttggagga acgggtgtcg ttgaaggatt
    17761 tgagtcaact acaggaccaa gtgcgctacc gtctaataac tgggctttga gcacagggaa
    17821 ttggttggtg tttgataatg gcacaccagg aaatttccgt tggggaattt ctaccgttac
    17881 tacaacgcca acacaagtgt atcaaggagc taatgcagct tatatgaatc gggagaccaa
    17941 cttttcaacg ggtcaaactt atgaggatta tttggcgtca ccagctttta cagttccagc
    18001 aaatggccaa cttcgttttt ggacaagagg ttttttaatt ggaaaccagg gaaccgttta
    18061 tcaaataaaa gtcgcaccag ctgccgctaa tccaacggat ccaaactctt atcttttggt
    18121 tcaacaatgg actgatgccg atttggtagc aagcgttagt caatatgaag aaaaagtagt
    18181 taatttgact gattatgttg atcttaatgt tcgtattgct tttgtgatgc aacaagctcc
    18241 aatcactgct ccatttggag atagatggtt tgttgataat gtacaagtag tggcgcagtg
    18301 ttttgatcct actgcattaa cagcaacacc agccattact tcggctcaat tgagttggac
    18361 gaatccagga acaacctcat gggaagtaga acttgttcct gcggcaggaa ctccaactgg
    18421 aataggaact ccatttaacg gagcagtacc ttcttttaat gttacaacaa ctttacctgc
    18481 aggtgtgcca ctaacggcaa caacagcata taaatattat gtgagagctg tttgttcagt
    18541 cggtgtcacc agtcaatgga ttggtccttt taatttttca acgctatcac ccggattaag
    18601 ttgtgaagca tcaatagtaa ttaataattt accatatagc acaactgaca gtacggcaaa
    18661 ctatggggat aaccccaata tagaaggaag tcctggaaca ggttgtaata ttacaggcaa
    18721 ttatttgaat ggtaatgatg ttgtgtattc ttatactgca ccagcaaatg gcaccataaa
    18781 tgtaaccatg acaccaactg gaacaaattc aggtgttttt gcttatgata gttgtgctaa
    18841 tgtaggtgta acttgtattg gcggtgctgc aggaacaaca acaactgtta gaacatttga
    18901 cttgtctgta actgctggtc aaacctatta ttttgttata tcaagtataa atacaacaca
    18961 aaccgttgga tataccttac tgatacaaaa tgtaaattgt gcgccaccaa ctgctttagc
    19021 ggcaaatgct ttgcaaacaa cagcaaattt aagctgggct caaggaggct cttcttcatg
    19081 ggaagtagtt gttcaacctg caggagctgg agttccagca ggagcaggta cacaaacaaa
    19141 tattaacaca aattatccag tgccaacagt tttaactcct aataccaatt atgagtttta
    19201 tgtaagagcc gattgtaatg atggtactgg caacttcagt gcttgggcag gaccatttgc
    19261 attcagaacg ttatgtgatg cttttactgt tccttttcaa gaaggattca acagtacttc
    19321 tacctcagaa gcttgttgga ctgttttaaa tgttaatgga gatacagatg catgggatat
    19381 gaactatgct accaatcctt ttgaaggaga tcaagtagca atgatgtaca cagattttaa
    19441 tgcaggagta aatgatgatt ggttaatttc accacaaata atccttacag gaaatcaaag
    19501 attgagatac cgttatagag ttcaatctgc aggtgaaccg aatgacttta gagtaatgct
    19561 ctctacaaat ggtacaagtc cagcgaattt cacgactact ttggtgccat tagcttctta
    19621 taacaatatt acctatatgg aagccattgt caatctttct gcttattcag gacctgtaaa
    19681 tattgcatgg catgtaccac caggaggttt agatggatgg agattgtata ttgataatgt
    19741 catcatagaa gatttaccaa cttgtccaga gccaagtatg ttaactgcaa cggcagtatt
    19801 atcaacttcg gcaacactga catggaccaa tggaaacgca gaaacagagt ggcaagtatt
    19861 agcattacca tgtggctcgc cagcaccagc tgcaggagca acaggttttg taacagttcc
    19921 tcctactggt tctc
//
LOCUS       DAOOBH010000032        18851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_3886_length_18851_cov_5.165461, whole genome shotgun sequence.
ACCESSION   DAOOBH010000032 DAOOBH010000000
VERSION     DAOOBH010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 18851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18851
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3886_length_18851_cov_5.165461"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PK218_05180"
     CDS             <1..494
                     /locus_tag="PK218_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPW97933.1"
                     /translation="KEKTVMVGGAAMYPSKNIVQNAVNSKDHTTLVAAVKAAGLVETL
                     ESKGPFTVFAPTNAAFDKLPKGTVETLLKPENKKMLQTILTYHVVSGKMNSTDIAAAI
                     KKGNGKAMLKTVSGGTLTAWMKGKDLYLTDENGKSAKVTIADVNQSNGVIHVVDAVVT
                     PKS"
     gene            complement(539..2959)
                     /gene="pheT"
                     /locus_tag="PK218_05185"
     CDS             complement(539..2959)
                     /gene="pheT"
                     /locus_tag="PK218_05185"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPW97934.1"
                     /translation="MKISYNWLKQFIKIDLKSEETAAILTDLGLEVEIVEKYQSIPGG
                     LEGIVVGHVLSCEKHPDADRLKITKVDLGDGTPVQIVCGASNVDAGQKVPVATIGTKL
                     FDKDGVAFEIKKGKIRGQESHGMICAEDELGLGTSHDGIMVLDEKLKPGTPCAKVFNI
                     ENDEVFEIGLTPNRADAMSHYGVARDLRASFLQKNPNIELISPSVSTFRIDKRTLKID
                     VDVKDSKLAPRYCGVTISGITVKKSPEWLQNRLKAIGLTPKNNIVDVTNYILHDLGQP
                     LHAFDASKVNGKIIVKTATAGAKFTTLDDVERTLHEEDLMICDEKGPLCIAGVFGGKN
                     SGVTETTNAIFLESAYFNPVSVRKSAKRHGLNTDASFRFERGIDPSITEFALKRAALL
                     IKEIAGGEITSDIVDIYPKKIEDFAVFLSFDKTAKLIGQELPKETIKKILASLDIKIN
                     SISDAGLGLTIPSYRVDVQREVDVIEEILRVYGYNNIQFTKKINATVANSPRTEDYKV
                     QNIVSSQLNSFGFHEMMANSLTTIDYIALSDDLKEEFTVSMLNPLSNDLAVMRQSMLF
                     SGLEAVSYNINRRKGDLKLFEFGKTYHKLPSGYDEPKHLTLFVTGNSNAESWANQQKP
                     SDFFLFKSYVLALLNRLGLQKTKNIPTENDVFGEGIAIAYGNDTLVEFGTIKKSILKH
                     FDIKQEVFYADFNWNLVLKLIGGKIKFTDIPKYPEVRRDLALLVDTNVSFDSLYAIAR
                     QSEKSLLKDINLFDVYEGKNLPEGKKSYALSFVLQDNSKTLTDEQIDKILNKLQKNFE
                     NELGASLR"
     gene            complement(3028..3891)
                     /locus_tag="PK218_05190"
     CDS             complement(3028..3891)
                     /locus_tag="PK218_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HPW97935.1"
                     /translation="MVHYNPKDWITFIFRFHKSDTFRQLIPMMLVIGFYTYLICFFEI
                     EYWKLAETSHVKNISVMHGLLSFVISLLLAYRTNTAYDRWWEGRKLWGSLVNNSRNLA
                     LKLSAILKSENDKDFFRKLIPSYAAILANHLSNEDVSKMLFDDFDLDIDHNKHQPNQV
                     AKRIFKKINDLYNEGKISGDQLIIINSEIQSFTDICGACERIKNTPIPYSYSAFIKKF
                     IFFFIMTLPFGYAFTLGYFSIPVVIFTFYVLASLELIAEEIEDPFGNDANDLPTKKIA
                     SNIKKHVEELL"
     gene            complement(3940..6480)
                     /locus_tag="PK218_05195"
     CDS             complement(3940..6480)
                     /locus_tag="PK218_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HPW97936.1"
                     /translation="MNNLLQTPIEYLKGVGPQRGELLRKEVGIHKYSDLLNFFPNRYI
                     DRTRYYKINELNNTIADVQIIGKISNIKTVEFGKAKKRLVATFIDETGEMELVWFQGQ
                     KWIRENLKLNIPYVIFGKVTAFNSKYNMSHPEMEVLNEHEKSLRSAMQPVYPSTEKLT
                     NRGISNRVVNKMMQQLFIETQANFSETLPNYLLEDLKLIPKNAALFNIHFPKSAELLA
                     KAQFRLKFEELFFIQLQLITKNLVRKHKIKGHPFSIVGDNFNNFYQNHLPFELTNAQK
                     KVLKEIRNDLGSNAQMNRLLQGDVGSGKTIVALMAMLIALDNGFQSCIMAPTEILAIQ
                     HYNGLTELAKELNINIKILTGSTKTSERKIIHEALEDGSLHILIGTHALLEDKVQFQN
                     LGLAIIDEQHRFGVEQRSKLWKKGSPPQFSQREEVKKVMYKYMTARPSTYNLIKELQI
                     KNKKESTKAEKILWEALRTKKIGFKIRRQHIIDLFIADFVCLEKNLIIEVDGGYHISA
                     AQIEADNLRTQILNEIGFKVIRFTNEQVINNLDSVLENITQTLESLSSGEVGEALPPH
                     ILVMTATPIPRTLAMSLYGDLDVSVIDELPPGRKPIQTVHRYDSNRLKVWKFIRDEIA
                     KGRQIYIVYPLIQESETMDYKDLMDGYESISRDFPLPKYSISIVHGKMKPADKEAEMK
                     RFSEGKTNIMVATTVIEVGVNVPNASVMIIESAERFGLSQLHQLRGRVGRGAEQSYCI
                     LITSHKLSNDSKIRLDTMCKTNDGFEIAEVDLKLRGPGDIMGKQQSGVLNLQIADIVK
                     DKDILMVARNEALKLLKKDPSMSLPEHQTLKTVFLELSKKKNIWNYIS"
     gene            complement(6853..7761)
                     /locus_tag="PK218_05200"
     CDS             complement(6853..7761)
                     /locus_tag="PK218_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPW97937.1"
                     /translation="MAKNDRHKDAEIFAEILRKTKLPNEITIGKFILKGTKGVKDKIQ
                     YNQNGFESLQNEHEYTFPILDGDLQVSLQLYENTSDFYRLFCYTKINGKEGMTFSVNL
                     TTEKESDNVIYLTQKIKFSEQYKGSEKLAQAHRRQKQIVFCEQLKKLGFDVTDNNDII
                     LGIFDPTKKQLVNTSLEKFLNDFIVVSILKGHFQGNKGYQLEILPTYNKLDYIFTGND
                     DEIENLPLKVIENKTKRNIPLGMRYKVFKKDNFKCVACGKNANDGAKLHVDHKIPFSL
                     GGLTELNNLQTLCSECNLSKSNKHID"
     gene            complement(8295..9080)
                     /locus_tag="PK218_05205"
     CDS             complement(8295..9080)
                     /locus_tag="PK218_05205"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HPW97938.1"
                     /translation="MTFIAFSVSYAQQQLPDFQLRINPSVDYKFAKKWQASFEYRYAL
                     DHDISEFRNSAVEAGIGYDITKSASIETRYRFTTSFTKDNHLLIAIFKYKKDINKRFS
                     LKFSSRYQFSTGSFDAGYMQYYKKPTQLLREKVTLEYNVPKSKASLYVAPEIFFKIGD
                     KDLPFLSYNTMRYNAGIDYKLNYGNSIGLGFFYEDVYNPKKTDRFVFTTKYNLSIDDL
                     LKTIKRKRDEKNGIEHISKKQKKKLKKKLKKELEEKETQLDSL"
     gene            complement(9097..9750)
                     /locus_tag="PK218_05210"
     CDS             complement(9097..9750)
                     /locus_tag="PK218_05210"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPW97939.1"
                     /translation="MEEVVANAKDVLNIVDFGTRAALNIIALALLVSLIYYPKHKNKD
                     FIFTFVIFNIINFMICYLLGSAKIKIGFAFGLFAIFSIIRYRTILVSIKDMGYFFICV
                     ALGMLNSLASVKDDFVMLIICNAVILVLTFFLERLDFLKNENSKDILYDKINFIKPQM
                     RAELIGDLSERTGLPIHRVEIVSIDYLKDVASVKAYYYSTESDSLFEETNDKDDKYD"
     gene            complement(9750..10502)
                     /locus_tag="PK218_05215"
     CDS             complement(9750..10502)
                     /locus_tag="PK218_05215"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPW97940.1"
                     /translation="MIDQFLNKVATMPPVSLAELDSVALQNRIDSKYVLNEDGLMALM
                     PCLSDNYKVLSINGLRSFTYENNYFDTKDLLFYKDHHNGYVNRIKVRSRKYVESNLCY
                     FEIKKKEKVNRTNKHRVSLDKIASEINNEKQDLVQSFTRKNLNDLKLILKNNFKRITF
                     VNNDFTERVTIDFNLQFSDSSNEVNLDTIIVLEIKQSKSTQESPLVTFLRKSNTRENS
                     FSKYIFGVISLHPEMKKNNFLPIIKNLTKIQK"
     gene            complement(10519..10908)
                     /locus_tag="PK218_05220"
     CDS             complement(10519..10908)
                     /locus_tag="PK218_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97941.1"
                     /translation="MKKHFIIICTLLIVVMTSCSKEEGYGGLATIQGKVYGKDFNSHG
                     ALVSEGYVGDMKVYISKHDNPNYFDKVDTSYDGSYKFEYLQKGTYDVWVFGDCDSCAW
                     AQVFAIETVEITSKRETVEVDDLVITF"
     gene            complement(11689..12756)
                     /locus_tag="PK218_05225"
     CDS             complement(11689..12756)
                     /locus_tag="PK218_05225"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPW97942.1"
                     /translation="MSLKNTNEIDIVRVKTSKIDTVDFENLTFGNVFTDHMLVCDFKD
                     GAWEKPLIKPYEPFMIDPSAKVFHYGQAIFEGMKAYKDEHDDVWLFRPDQNFERFNKS
                     AIRMAMPEVPESIFMDGLKAVVDLDRDWVKKGMGNTLYLRPFMIAIGSGVIAQPSTQY
                     RFMIILSPAKSYYSGEVKVIIAEHYSRAANGGIGAAKAAGNYSAQFYPQKLAQEKGFQ
                     QIIWTDDATHTKLEEAGTMNVFFKINDTLYTAPTSERILDGVTRKSIIELAKSEGIKV
                     EERSVLVNELVTAAKDGSLTEIFGAGTAAVVSPIVGFQYKEHYYELPKQENATALIIK
                     DKLTKLQYKLAEDKFGWTVKI"
     gene            12962..13450
                     /locus_tag="PK218_05230"
     CDS             12962..13450
                     /locus_tag="PK218_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HPW97943.1"
                     /translation="MKLFLVAFTLVVSAATFAQSKSKATISTANYALFGEKFQPNKIL
                     SKKEMLKKYKNLKKGDTISVQYASTIKSVCKKKGCWMKMDLTNDETSFVKFKDYEFFV
                     PLNADGNEAIVYGKAFVDVVSVDELKHYAKDGGKSKDEINKITQPEVTYSFEASGVYI
                     KK"
     gene            13462..13914
                     /locus_tag="PK218_05235"
     CDS             13462..13914
                     /locus_tag="PK218_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97944.1"
                     /translation="MKNFLLFGLALLLFSCHKEVKKDAATTEKKCDTTSGKKLEMYQM
                     TEMALLMEQMYVDNKRLKERIIKGDTIGTFPQHFLKIHTATMTDESENDAFFKEQAAK
                     YIKAQQLIYADPKNAKEHFNNGVDACLNCHEQKCGGPIPRIKKLYIIV"
     gene            13917..14582
                     /gene="mnmD"
                     /locus_tag="PK218_05240"
     CDS             13917..14582
                     /gene="mnmD"
                     /locus_tag="PK218_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HPW97945.1"
                     /translation="MKREIIQTNDGSTSIYLPDWNESYHSKHGAIQEANHVFIKNGLS
                     LFEGKPVSILEIGFGTGLNALITYYESLKNQQTIHYVGVEAYPVSIEEVVQMNYPTEI
                     NSSLNDAFQLLHTSNWETEIQFNSNFTFKKRKQFFQEIKDSNTFDLIYFDAFGYDKQP
                     ELWSVVIFKSMFDALKKNGILVTYACRSILKRNMIEAGFKVEKLPGAPGKREMLRAIK
                     AEV"
     gene            14693..14890
                     /locus_tag="PK218_05245"
     CDS             14693..14890
                     /locus_tag="PK218_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97946.1"
                     /translation="MSKSMLTFTKKVLKGVSFDVQLFCKELEKAYKVLLPHELSELSE
                     WVLQFTQNKPELRQCLVRIKY"
     gene            15101..15295
                     /locus_tag="PK218_05250"
     CDS             15101..15295
                     /locus_tag="PK218_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97947.1"
                     /translation="MPRMIYDYTKSVLERVSFDPILFSKELKKAIRNLLPYEIEQLKK
                     WLQFFTKEKPELKRCLTVVS"
     gene            complement(15369..16310)
                     /locus_tag="PK218_05255"
     CDS             complement(15369..16310)
                     /locus_tag="PK218_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HPW97948.1"
                     /translation="MTITQLQYVLAVAEHKNFTLAAEKCFVTQPTLSMQIQKVEEELS
                     IQIFDRTKKPIQLTEIGQKIVNQAKNIVNEANRITDIVEQQKGFIGGEFRLGIIPTVM
                     PTLLPMFLKNFIKKYPKVHLIIEELNTEEIIQRLNNGHLDAAIAATPLKEEKIKEVVL
                     YFEPFVAYIPENHKSFPKNEIEVEDLDLNEILLLQDGHCFRDGILNLCKNVSNTEKNR
                     FQIESGSFETLIKLADEGLGTTLLPYLHTLDLKEKDKVKLRNFKDPKPAREVSIIYPK
                     NELKIHIIDALRTIISAVVKGAIVFQNVEIISPTQKK"
     gene            16511..16789
                     /locus_tag="PK218_05260"
     CDS             16511..16789
                     /locus_tag="PK218_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97949.1"
                     /translation="MKLFTRILLFLFILFLSTPTLVTLIEKDSDISMFYCFAEEEHHK
                     EIKAEIKFYVEHNVIAFMELEKKTSFIENNSEHPTVFREIFSPPPNFI"
     gene            16849..18444
                     /locus_tag="PK218_05265"
     CDS             16849..18444
                     /locus_tag="PK218_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPW97950.1"
                     /translation="MTKKINLFANLKSDFASGLVVFLVALPLCLGIALASGAPLFSGI
                     IAGIVGGIVVGYLSKSHLSVSGPAAGLTAIVLVAITDLGSFNAFLLAGLIAGGIQLLL
                     GFIKAGSISNYFSTNVIEGMLAGIGVIIFLKQIPHAFGYDKDYEGDLAFNQPDGQNTF
                     TELFSVLGHVQLGALLITIVSLFILIVWDKIPALKKLKLVPSALVAVFVGVLINEYFI
                     QSGSSLAVLDNHLVKLPVPTSLEELGAIIVTPDFSAITNSKVWIVGITIAVVASIETL
                     LCIEAADRMDEQKRYTDTNVELKAQGIGNIISSLLGGLPMTSVVVRTTANNTAGAKSK
                     MSAIIHGVLLLISVLAIPVLLNKIPLATLAAILLLIGYKLANPSKIKHFWEKGKYQFI
                     PFIATVIAVVFTDLLKGVALGIIISIIFIMKGNLQRAYKFRKEEYKDGDIIHIDLAQE
                     VSFLNKAAIKQTLSSIPENSKVIINASDTVYIAHDVLDLIKEFKKIRAKDENIKVKLE
                     GFKKAYNLIETGEEGKHVFIEHK"
     gene            18486..>18851
                     /locus_tag="PK218_05270"
     CDS             18486..>18851
                     /locus_tag="PK218_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HPW97951.1"
                     /translation="MNSLAIAQKSAIGNWFLYFGNQKINEKWNWHNEIQYRNYNFIGD
                     TNQLLLRTGIGYNLTENNNNLLLGYGYINTQNYIPDTDEKVHINEHRIYQQFITKEHF
                     GRFFLQHRYRIEERFVANEF"
BASE COUNT         6121 a         2953 c         2976 g         6801 t
ORIGIN      
        1 aaaaagagaa aactgtaatg gttggaggag cggcaatgta tccatctaaa aacattgttc
       61 aaaatgcagt taattcaaaa gatcatacga cattagtagc tgctgtaaaa gctgctggtt
      121 tagtagaaac attagagtca aaaggaccat ttaccgtgtt tgccccaacc aatgctgctt
      181 ttgataagct tccaaaagga acagtggaaa ctttattaaa accagaaaac aaaaaaatgc
      241 tacaaactat tttgacctac catgtagttt caggaaaaat gaattccaca gatattgcag
      301 ctgctatcaa aaaaggaaat ggaaaagcga tgcttaagac agttagtggt ggtactttga
      361 ctgcttggat gaaaggtaaa gatttatatc ttactgatga aaatggaaaa tcggctaaag
      421 ttactattgc tgatgtaaac cagtcaaatg gtgttattca cgttgttgat gcagtggtta
      481 ctcctaaatc ataacaatat atgaagaaaa aatgcctcac aattgtgagg catttttttt
      541 atcttaaact tgctccaagc tcattttcaa aattcttttg caacttattg agaattttat
      601 caatttgttc gtcggttaat gtctttgaat tatcttgtag aacaaaactc aaagcataag
      661 atttttttcc ttctggaaga ttttttcctt catatacatc aaaaagattg atgtctttta
      721 ataatgattt ttccgattgg cgagcaatag catacaagct atcaaaagaa acatttgtat
      781 ctactaataa cgctaaatct ctgcgaactt ctggatattt tggaatatca gtaaacttaa
      841 tttttccgcc aataagctta aggactaagt tccagttaaa atcagcataa aaaacttctt
      901 gttttatatc aaaatgtttt aaaattgatt ttttaattgt tccaaattca accaaagtat
      961 catttccata agcaatggct attccttcac caaaaacatc attttcagtt ggtatatttt
     1021 ttgttttttg caagccaagt cggtttaata atgctaaaac ataactttta aataaaaaga
     1081 aatctgaagg tttctgttgg ttagcccaac tttcggcatt actatttcca gttacaaaaa
     1141 gcgttaaatg tttgggttcg tcatatccag acggtaattt atgataggtt tttccaaatt
     1201 caaataattt taaatcacct tttcttctgt ttatattata agaaacagct tctaatcctg
     1261 aaaacaacat tgattgtcgc atcactgcta aatcattact taaaggattc agcatagaaa
     1321 cggtaaattc ttcttttaaa tcgtctgaca aagcaatata atcaatagtt gttaacgagt
     1381 ttgccatcat ttcatgaaat ccaaacgaat ttaattgaga agaaacaata ttctgtactt
     1441 tgtaatcttc cgttcttgga gaattggcaa cagtagcgtt gatttttttg gtaaattgaa
     1501 tgttattata gccataaact cttagtattt cttctataac gtcaacttca cgttgtacat
     1561 caactctata cgagggaatt gtcaagccca acccagcatc cgaaatactg ttgattttta
     1621 tgtctaaaga agctaaaatc tttttaatag tctctttcgg tagttcttga ccaattaact
     1681 tggcggtttt atcaaaactc aagaaaacag caaaatcttc aatcttttta ggatatatat
     1741 ctacaatatc agaagtaatt tcgccacctg caatttcttt aattaatagt gcagcacgtt
     1801 ttagtgcaaa ttctgtaatg cttggatcaa ttcctctctc aaaacggaag gaagcatccg
     1861 tgtttaagcc atgtcttttt gctgattttc tgacgctaac aggattaaaa taagcacttt
     1921 ctaggaaaat agcatttgtg gtttcagtaa ctccagaatt ttttcctcca aaaactccag
     1981 caatacataa tggacctttt tcgtcacaaa tcattaaatc ttcttcatgc aaagtgcgtt
     2041 caacatcatc taaagtggtg aattttgctc ctgccgtagc tgtttttaca attattttac
     2101 catttacttt tgatgcatca aacgcatgta aaggttgccc taaatcgtga agaatgtaat
     2161 tagtgacatc aacaatatta ttttttggtg ttaaaccaat cgcttttagt cgattctgta
     2221 accattctgg agattttttt acagtaattc cagagatagt aacaccacaa tatcttggtg
     2281 ctaatttgct gtctttgaca tcaacgtcaa tttttagtgt tcttttgtca attctaaaag
     2341 tactcaccga aggagaaatt aattctatat tcggattttt ttgaaggaaa ctagcgcgta
     2401 aatctctagc cactccataa tgtgacatgg catcagcacg atttggcgtt agaccaattt
     2461 caaaaacttc atcattttct atattaaaaa ctttggcaca tggtgttccg ggttttagtt
     2521 tttcatccaa aaccataata ccatcatgac ttgttcctaa accaagttca tcttcggcac
     2581 aaatcattcc gtgactttct tgtcctcgaa tttttccttt cttgatttca aaagcaacac
     2641 catctttgtc aaaaagtttg gttccaatag tggcaacagg aactttttgt cctgcatcaa
     2701 cattgcttgc gccacaaaca atttgaacag gagttccatc tcctaaatca acttttgtaa
     2761 tttttaatcg gtcggcatcg ggatgttttt cgcaagatag tacgtgtcca actactatgc
     2821 cttctaaacc gcctggaatt gattggtatt tttcaactat ttcaacttca agtcctaaat
     2881 cagtaagaat tgccgctgtt tcttcggatt ttaaatctat tttaatgaat tgttttaacc
     2941 aattgtatga aatcttcatt ttctctttta tataaggtgg caaagatagt catattcaaa
     3001 aaatatagca attggaaata gcaattatta taacaattct tcaacatgtt ttttaatgtt
     3061 ggaagcaatc tttttggtag gtaaatcatt ggcatcattg ccaaacggat cttcaatttc
     3121 ttcagcaatt aattccaaac ttgctaatac ataaaaggtg aaaataacca ctggaatact
     3181 aaaataacct aaagtgaaag cataaccaaa aggcaatgtc atgataaaga aaaagataaa
     3241 ttttttgata aacgcactgt atgaataggg tataggcgtg ttttttatac gctcgcacgc
     3301 accacaaata tcggtgaacg attgtatttc gctattgatg ataattaatt ggtcaccaga
     3361 tattttacct tcattatata aatcatttat tttcttaaaa atacgttttg ccacttgatt
     3421 gggttggtgt ttgttgtgat caatatctaa atcgaaatca tcaaaaagca ttttacttac
     3481 atcttcattt gaaagatgat ttgctaaaat agcggcataa cttggaatta gttttctgaa
     3541 aaaatcttta tcattttccg attttaaaat ggcacttaat ttaagagcta aatttcgact
     3601 gttgttgact aaacttcccc ataattttct gccttcccac catcggtcgt atgcggtatt
     3661 ggttctgtat gccaataaaa gtgaaatcac aaaacttaat aatccatgca ttacggaaat
     3721 gtttttgacg tgactagttt cggctaattt ccaatattcg atttcaaaaa agcagattag
     3781 ataagtgtaa aacccaatga ccaacatcat aggaatcaat tgtctgaagg tatctgattt
     3841 gtgaaatctg aaaataaaag ttatccagtc ttttgggttg taatgtacca tttgaggtta
     3901 gaatttagaa attagagttt aggtaattaa tatttttatt taactgatat aattccagat
     3961 atttttcttt tttgaaagtt ctaaaaatac agtttttaac gtttgatgtt cgggtaatga
     4021 cattgatgga tctttcttta ataatttcaa cgcttcgttt cttgcaacca tcaaaatatc
     4081 tttgtctttt acgatgtctg caatttgaag attgagtaca ccactttgtt gttttcccat
     4141 gatgtcgcct ggtccacgca gttttaaatc aacttctgca atctcaaagc catcattggt
     4201 tttacacata gtgtccaaac ggattttgct gtcgtttgat aatttatgcg acgttatcaa
     4261 gatgcaataa ctttgttcgg caccacgacc aacacggcca cgtaattgat gcaattggga
     4321 taacccaaaa cgttcggcac tttcgataat cattacacta gcgttgggaa catttacgcc
     4381 cacttcaatc accgttgtag ccaccataat attggtcttt ccttctgaaa aacgtttcat
     4441 ttcggcttct ttgtcagcgg gtttcatttt tccatgtact atagaaatag aatattttgg
     4501 cagcggaaaa tctctagaaa tactttcata accatccatt aaatctttgt aatccatcgt
     4561 ttcgctttcc tgaattaagg gatagacaat ataaatttgt ctgccttttg caatttcatc
     4621 tctaatgaat ttccatactt tcagtcggtt gctgtcatat cgatgcacgg tttgaatggg
     4681 ttttcgtcct ggtggcaatt catcaatcac cgaaacatcc aaatcgccat acaaactcat
     4741 ggctaacgtt cgaggaattg gcgttgccgt cattactaat atatgaggag gaagagcctc
     4801 tccaacctct ccagaggaga ggctttctaa agtttgcgta atattttcta gtacagaatc
     4861 aaggttattt attacttgtt catttgtaaa tcgaatgact ttaaagccaa tttcatttaa
     4921 tatttgtgtc cgtaagttat ctgcttcaat ttgtgctgcc gaaatatgat aaccaccatc
     4981 aacttcgata attagatttt tttcaagaca aacaaaatca gcgataaata ggtctataat
     5041 gtgttgtcgt ctaattttaa aaccaatttt tttggttcgt aaagcttccc aaagaatttt
     5101 ctcagcctta gtactttcct ttttgttttt aatttgaagt tctttaatta aattatatgt
     5161 cgaaggtctt gcggtcatgt atttatacat tactttttta acttcctctc tttgggagaa
     5221 ttgaggtggg cttcctttct tccagagttt acttctttgt tctacgccaa atctgtgttg
     5281 ttcgtcaata atagccaatc ctaaattttg gaattgtact ttgtcttcta acaaagcatg
     5341 tgttccaatt aatatgtgca aacttccatc ttccaaagct tcgtgaatga tttttcgttc
     5401 cgaagttttg gttgaaccgg ttaatatttt gatgttgata ttcagttctt ttgccaattc
     5461 ggtcaaacca ttataatgtt gaatggctaa aatttctgtt ggcgccatta tgcagctttg
     5521 aaaaccattg tccaaagcga ttagcattgc catcagcgcc acaattgttt ttcctgagcc
     5581 cacatcgcct tgtaataagc ggttcatttg cgcattgctt cctaaatcat ttctgatttc
     5641 tttcagcact tttttttgtg cattggttag ttcaaatggc agatggtttt ggtaaaagtt
     5701 attaaagtta tcgccaacga tagaaaaagg atgccctttt attttatgtt ttctaactaa
     5761 attttttgtg attaattgca gttgaataaa gaacaattct tcaaatttta atcgaaattg
     5821 tgctttggcc agtaattcgg cgctttttgg gaaatggatg ttgaataagg ctgcattttt
     5881 tggaataagt tttaagtcct ccaatagata attgggcaat gtttccgaaa aattagcttg
     5941 cgtttctata aataattgct gcatcatttt gttcaccaca cgattggaaa taccacgatt
     6001 ggtcagtttc tctgtggatg gataaacggg ttgcattgcc gaacgcaaac ttttttcgtg
     6061 ttcgtttagt acttccattt ctggatgcga catattgtat ttactattga acgcagttac
     6121 ttttccaaaa atcacatacg gaatattgag ttttaaattt tctctaatcc atttttgacc
     6181 ctgaaaccac acaagttcca tttctccggt ttcgtcaata aaagtggcga ccaatctttt
     6241 ttttgctttg ccaaattcta cggttttgat gttacttatt ttgccaataa tttgcacatc
     6301 ggcaatagta ttgtttaact catttatttt gtaataccgt gttctgtcaa tgtatcgatt
     6361 agggaaaaaa ttcaataaat cgctatattt atgaatgccc acttctttgc gcaacaactc
     6421 cccacgctgc ggaccaacgc ctttgaggta ttcaatgggt gtttgaagaa ggttattcat
     6481 gatcaaattg cgaaactgct tttttagtaa agcgaagata gtttttaatt tgaagattta
     6541 aaatggatgg ggttttaaat gtagcataag tggctataaa ctcctgtttt ggggaatggt
     6601 cattagattg tatttaggta ttttaggaaa agattatggg atgtgaaaat taagttgctg
     6661 cgaagtcaga gatattcgcg gagcagttgg agttgtattt gaattgcaaa cgtatcacaa
     6721 caacacgtct attgcgtgca aacacaaaat tatatttcat tttaacacga aattgaaagg
     6781 tacaaaacaa aatttaaatt aatcacaaat gttgcaatag ctcgtaattg ctgttagttg
     6841 atggtgttgt tgttaatcta tatgtttgtt acttttactt aaattacatt cactgcataa
     6901 agtttgtaaa ttgtttaatt cggttaaacc tcctaatgaa aatggaattt tatggtcaac
     6961 gtgaagttta gctccatcgt ttgcgttttt tccacaagct acacatttaa aattatcttt
     7021 tttaaatact ttgtatcgca ttcctaaagg aatatttcgt ttagttttat tttcaattac
     7081 tttcagcggt aaattttcta tttcgtcatc gttacctgta aaaatgtaat ctaatttatt
     7141 ataagttggt aatatttcaa gttgataacc tttattgcct tgaaaatgtc ctttcaaaat
     7201 tgaaaccaca ataaaatcat tcaaaaattt ttctaatgat gtgtttacaa gttgcttttt
     7261 ggttgggtca aatattccta aaataatatc gttattgtct gttacgtcaa atccaagttt
     7321 tttaagttgt tcgcaaaata caatttgttt ttgtcttcta tgagcttgag ctaatttttc
     7381 acttccttta tattgttcgg aaaattttat tttttgagtt agataaatta cgttatcact
     7441 ttctttttca gttgttaagt ttacagagaa agtcattcct tcttttccat taattttcgt
     7501 gtaacaaaat aagcgataaa aatcactcgt gttttcgtaa agttgcaatg aaacttgtaa
     7561 atctccatct aaaataggaa atgtgtattc gtgttcgttt tgtaagcttt caaatccatt
     7621 ttgattatat tggattttat cttttacacc ttttgttcct tttagaataa attttccaat
     7681 agtaatttcg ttcggaagtt ttgtttttct taaaatttcg gcaaatattt cagcgtcttt
     7741 atgtctatcg tttttcgcca tttttctttt tttacgttct gttcaaatac actatcagct
     7801 aactagtaaa tatccacaag tttatacgac tttttataaa tactgtttcc caaaaataac
     7861 caaaaaacca tttccaataa caaatttgcc ttacaatttt taactgtttt taaacatttt
     7921 atcaccttta ttagatttaa aataaaagtc gcccaccatt tcggcaacgg tgctgacgaa
     7981 aaagtgaacg gaatccatcc cgtggggaac ggtgctgacg aaaaagtgga cggaatccat
     8041 ctcgtgggga acgttgctga cgaaaaagtg aacggaatcc atcccgtgga gaacggtgtc
     8101 taaatggtgg gtgacttttt taaggtcttc aaattaatag aagaaaactt caagaaatac
     8161 gtttgcgtct tcaaattttt tgggacatct ttaaactatt aggagatgac ttttaggtaa
     8221 taaaatcaag taattattct tttgtgaagg ttaatcaaat aaaaaaagtg ccaaatttct
     8281 ttgacacttt tctattataa tgaatctaat tgggtttctt tttcttccaa ctcttttttc
     8341 aacttctttt tcagtttctt tttttgtttc ttgctgatgt gttcaatacc atttttttca
     8401 tctcttttcc ttttgatagt tttcaacaaa tcatcaatag aaagattgta tttggtagta
     8461 aaaacaaatc gatccgtctt tttgggattg taaacatctt cataaaaaaa gcctaaacca
     8521 atgctgtttc cataattcag tttataatca atacctgcat tatatcgcat tgtattataa
     8581 ctaaggaatg gcaagtcttt atcaccaatt ttgaagaata tttcgggtgc tacatataat
     8641 gatgctttcg attttggaac attgtattct aatgtaactt tttcacgcaa taattgggtt
     8701 ggttttttat aatactgcat gtatccagca tcaaaacttc cggtactaaa ttgataacgc
     8761 gaactaaatt ttaaagaaaa ccgtttgttg atgtcttttt tatacttaaa gatggcaatt
     8821 agcaaatggt tatcttttgt aaatgacgtt gtaaaacggt atctggtttc aatacttgca
     8881 ctttttgtaa tatcataacc aattccagct tcgacagcac tgtttctaaa ctcactgatg
     8941 tcgtggtcta aagcatatcg atattcaaat gaagcttgcc atttttttgc aaacttataa
     9001 tcaacagatg gattgattct caattgaaaa tcgggcaact gttgttgtgc atagcttacc
     9061 gagaaggcta taaaagtcat gagaaagtat aacttcctag tcatatttgt cgtctttgtc
     9121 atttgtttct tcaaacaagc tatcactttc tgtagaatag taataggctt ttacggaagc
     9181 aacatctttt aagtaatcaa tagaaacaat ctcaacacga tgaatgggca atccggttct
     9241 ttcggataaa tcgcctatta gttctgctct catttggggt ttgataaagt taattttatc
     9301 atacaaaatg tcttttgaat tttcattctt taagaaatca agacgctcta agaagaatgt
     9361 taacactaaa ataacagcat tacaaatgat tagcattaca aaatcatctt taactgatgc
     9421 caaagagttt agcataccca aggcaacaca gataaagaaa taccccatgt ctttaataga
     9481 aacgagtatg gttctgtatc gaatgataga gaaaatggca aacaaaccga aggcaaaacc
     9541 aattttaatt tttgcagagc ccaaaaggta gcaaatcata aaattgataa tgttgaagat
     9601 tacaaatgtg aagatgaaat ctttgttttt gtgttttgga taataaatta acgatactaa
     9661 taacgcaaga gcgataatat taagtgcagc tcgggttccg aaatctacaa tgtttaaaac
     9721 gtcttttgcg ttagctacta cttcttccat tatttctgaa ttttggttaa gtttttaatt
     9781 atgggtaaaa aattattttt tttcatttct gggtgaagcg aaatcacacc aaaaatatat
     9841 ttgctaaagc tattttcgcg agtgttgctt tttctcaaaa aagtaactaa aggcgattct
     9901 tgtgttgatt ttgattgttt gatttccaaa acaataatag tatccaagtt aacttcatta
     9961 ctactgtctg aaaattgaag attgaagtca atggtaacac gctccgtaaa atcattatta
    10021 acaaaagtga ttcttttgaa attgtttttt agaataagtt ttaaatcatt taaattttta
    10081 cgtgtaaacg attgtactaa atcttgtttt tcattgttta tttcagaagc tattttatct
    10141 aaagaaaccc tgtgtttgtt agttcggttt actttttcct ttttcttaat ttcaaaataa
    10201 cataaattgg actctacata ttttctacta cgcactttaa tgcgattcac atagccatta
    10261 tgatggtctt tgtaaaacaa taagtctttg gtatcaaaat aattattttc ataggtaaaa
    10321 gaacgaagtc cattgataga gagaacttta taattatcgg atagacatgg cattagagcc
    10381 atcaatccat cttcattcaa tacatattta ctatcaattc gattttgcaa agcaacagag
    10441 tctagctccg ctaaagaaac gggtggcata gttgctactt tatttaaaaa ttgatcaatc
    10501 ataatttctt tttttttttt agaaggtgat aactaaatca tctacttcaa cggtttctct
    10561 ttttgaggtg atttctacag tttctatagc aaatacttgc gcccaagcac aagaatcgca
    10621 atcgccaaaa acccaaacat cataagttcc tttttgaaga tattcaaatt tatatgagcc
    10681 atcatacgag gtatctactt tatcaaaata atttggatta tcgtgtttgc tgatatatac
    10741 tttcatatcg ccaacataac cttccgagac caaagctccg tgcgaattaa aatcttttcc
    10801 ataaactttt ccttgaatgg tagccaaacc accataacct tcttctttag aacaagaagt
    10861 cattacaact atcaataagg tacaaataat tataaaatgt tttttcataa cctaaattta
    10921 ttaattttaa aaatgttttt ttgtattgtg ttaatatgta atttttataa aatacatgtt
    10981 ttaaataata atgatttaat aacataaatt taacattaca aagaaattga ttttttttta
    11041 aactcgcatc tcgtttcaac gagtatatcc gctttttgac gttttttagt acgtttttaa
    11101 tgattttgaa taaaaaagta aattttagtg cactattttg ttaaaaaatt aacttgatac
    11161 tatattgttt ttttgtgcaa aatttcgaaa aaaaagattt aatattttac tattaaatct
    11221 ttttataact aaacataaca aacaattatt ttttgatatt cactttaaat atttttttgt
    11281 gtacgcattt aactattctc cgattgaaat tgttaataac aatggtcagc tactttaata
    11341 attaaaacac gtcctaaata tttgcttcaa tcaatcttta gacgtaaaat aattattaat
    11401 cttgcattta cttattaatt ttattttttt ggttttgcat ttacgatttt atgcaatatc
    11461 tatgttaaga aagttattag cgattactct tttaatgata aaataggaac gaagtgtaat
    11521 gcaaaatggt ttttgattat actttttcaa gaagaaattc tattgaaatc aatctacgca
    11581 aagaaggatt agactttgtt taaaaaaatt aaaagtattc gtcaaaaaaa aatcctcaac
    11641 catgaaagaa aatgattgag gattcgttga ttataaataa tcattgggtt atatttttac
    11701 agtccaacca aatttatctt cggcaagttt gtattgcaat ttggtcaact tgtctttaat
    11761 aattaaagca gtagcatttt cttgtttggg taattcgtaa taatgctctt tgtattgaaa
    11821 cccaactatt ggactaacaa cagcggctgt tcctgcacca aatatttcgg ttaaactacc
    11881 gtcttttgca gcagtaacca actcattaac caaaaccgaa cgttcttcta ctttaatacc
    11941 ttcacttttg gccaactcaa taatactctt acgggttaca ccatcaagaa tacgctcact
    12001 agttggagca gtatataagg tatcgtttat tttgaaaaat acattcatgg tgcctgcttc
    12061 ttctagtttg gtatgcgttg catcatccgt ccaaatgatt tgttgaaagc ctttttcttg
    12121 tgctaatttt tggggataaa actgtgcaga gtaattccca gctgctttag cagcaccaat
    12181 accaccattg gcagcacgac tataatgctc agcaataatc acttttactt cacctgagta
    12241 atatgatttg gctggagaaa gtataatcat aaaacgatat tgagtagaag gttgtgcaat
    12301 aacgccacta ccaattgcaa tcataaaagg tctaaggtaa agtgtgtttc ccattccttt
    12361 ttttacccaa tctctatcta aatcaacaac cgcttttaac ccatccatga atatactctc
    12421 gggaacttcg ggcatagcca ttctaattgc cgatttgttg aaacgttcaa agttttggtc
    12481 gggtctaaaa agccaaacgt catcatgctc atctttataa gctttcattc cttcaaaaat
    12541 agcttgacca taatgaaaaa ccttagcaga aggatcaatc ataaaaggtt cgtatggttt
    12601 aatcaaaggt ttttcccatg caccatcttt gaaatcacaa accaacatat ggtcagtaaa
    12661 aacgtttcca aatgttaaat tttcaaagtc aacagtgtct atttttgaag tttttactct
    12721 aactatatcg atttcgtttg tgttttttaa actcatttgt tacgtttttt tttgttttta
    12781 ttggtgtaag ttattgtttt acaatgtttt gcgcggcagt attcgctcgc tataccaacg
    12841 ttttataatt tttgtaaagc taataatttt tttttgggtt tttatactca taaataacat
    12901 aaattaatgg ctaaactttt ttacttttgt agctcttttt aaaattaaaa acatgaaaaa
    12961 aatgaaacta tttcttgttg cttttacttt agtagtgagt gcggcaactt ttgcacaatc
    13021 aaaatctaaa gcaacaatca gtacggcaaa ttatgctcta tttggagaaa aatttcagcc
    13081 aaataaaata ctttctaaaa aagaaatgtt aaaaaaatat aagaatttaa agaaaggtga
    13141 tactatttct gtacaatatg cttctactat taagtcggtt tgcaagaaaa aaggatgttg
    13201 gatgaaaatg gacttgacca atgatgaaac atcttttgta aagtttaaag actatgagtt
    13261 ttttgtaccc ttaaatgctg atggaaatga agccatcgtt tatggtaaag cttttgtaga
    13321 tgttgtatcg gttgatgaat taaaacatta tgccaaagat ggtggaaaat ctaaagacga
    13381 aatcaataaa attacccaac cagaagtaac gtattcgttt gaagcttcgg gtgtttatat
    13441 taagaagtaa tttaaagtaa aatgaaaaac tttttactat ttggattagc attgcttttg
    13501 ttttcttgtc acaaagaagt aaaaaaagat gctgctacta ccgaaaaaaa atgtgatact
    13561 acttcgggta aaaaactgga gatgtatcaa atgacagaaa tggcattatt aatggagcaa
    13621 atgtatgtag ataacaaacg attgaaagaa cgcattatta aaggtgatac tataggtacg
    13681 tttcctcagc attttttaaa aatccatacc gccacaatga cggatgaatc tgaaaacgat
    13741 gcttttttta aagaacaagc cgcaaaatat atcaaagcac aacaattgat ttatgctgat
    13801 cccaaaaatg cgaaagaaca ttttaacaat ggagtcgatg cttgtttgaa ttgtcacgaa
    13861 caaaaatgtg gcggaccaat tccgagaatt aagaaactgt atattatagt atagttttga
    13921 aacgtgaaat aattcaaacc aacgacggtt caacttctat ttatcttccc gattggaacg
    13981 aaagttacca ttccaaacat ggtgctattc aagaagccaa tcatgtattt ataaaaaatg
    14041 ggctttcctt gtttgaaggg aagcctgttt ctattttgga aataggtttt ggcacaggtt
    14101 taaatgcatt aattacatat tatgaaagtt taaaaaacca acaaacaata cattatgttg
    14161 gagtagaagc ttatcctgtt tcaattgagg aagttgtgca aatgaattat ccaacagaga
    14221 ttaattccag tttaaatgat gcatttcaat tgttgcatac ttcaaactgg gaaacagaaa
    14281 tccaattcaa ttctaatttt acgtttaaaa aaaggaaaca attctttcaa gaaattaagg
    14341 acagtaatac atttgattta atttattttg atgcttttgg atatgataaa caacccgaac
    14401 tttggagcgt agttattttt aaaagtatgt tcgatgcttt gaaaaagaat gggatattgg
    14461 ttacttatgc ttgccgctca atactcaaaa gaaacatgat tgaagctgga ttcaaagtag
    14521 agaaattgcc cggtgctcct ggaaaacgag aaatgttgag agcaattaag gcagaagttt
    14581 aacgttttga tttgttttat ttaacagtta aaaatcagtt tgtaaattta tattttatat
    14641 ttttgaatta atctaaaaaa ccaattgttt caccaactaa aattaattat ttatgtcaaa
    14701 atcaatgttg actttcacaa aaaaagtttt aaagggtgtt agttttgatg tgcagctttt
    14761 ttgcaaagaa ttagaaaaag catataaagt attattgcca catgaactga gcgaattatc
    14821 agaatgggta ttgcaattta ctcaaaacaa acctgagcta aggcaatgtt tagtaagaat
    14881 taaatattaa taagaaacta aggagtattt tactcctttt tttagtgctt gctttaaatt
    14941 tgtaagattt atcaatttaa aattgttaaa ttatgtagat aaacgtaaaa aaacgcactt
    15001 tatcgatgta tttcatcgat tttatttggt ggttagtaaa aattaaaata gttttacctc
    15061 agaatttaaa attaagataa acccctaaat cttaaccatt atgcctagaa tgatctacga
    15121 ttacacaaaa tctgtgctcg agagagtaag ttttgacccg attctttttt caaaagagtt
    15181 gaagaaggca ataagaaatc tgttgcctta tgaaattgaa cagctaaaaa aatggctcca
    15241 attctttaca aaggaaaaac cagagctaaa gaggtgttta acggttgtca gttaaaaaag
    15301 ttttttttca aaaaataagt gtttgaatta gttaaaatta gattgaaaat aaggagttgt
    15361 tcaactcctt attttttttg tgtaggacta ataatttcaa cattttgaaa cactatggca
    15421 ccttttacta cagccgaaat aatggttctc aatgcgtcga taatatgtat ttttaattca
    15481 ttttttggat aaataatact cacttctcta gcaggctttg ggtctttaaa attacgcagt
    15541 tttactttgt ccttttcttt taaatctagc gtatgcaaat agggtagaag cgttgttccc
    15601 aaaccttcat cagcaagttt tattaaggtt tcaaagcttc cactttcaat ttgaaaacgg
    15661 tttttttctg tattactaac atttttgcat aagtttaaaa ttccatctct aaaacaatgt
    15721 ccgtcttgaa gtaataatat ttcattcaaa tctaaatctt ctacttcgat ttcatttttt
    15781 gggaatgatt tgtggttctc aggaatgtat gcaacaaatg gttcaaaata aagcactact
    15841 tcttttattt tttcttcttt caatggagta gccgcaatag ctgcatcaag atgtccattg
    15901 tttaatcttt gaataatttc ttcagtgttt aattcttcga taattaaatg gacttttggg
    15961 tatttcttaa taaaattctt aagaaacata ggtaacaatg taggcattac tgttggaata
    16021 attcccaatc taaattctcc accaatgaat cctttttgct gttccactat atcggttatt
    16081 ctattggctt cgtttacaat attcttagct tgattaacta ttttttgacc tatttcagta
    16141 agttgaatag gtttctttgt tcgatcaaat atttgaatac tgagttcttc ttccactttt
    16201 tgaatttgca tgctcaatgt tggttgcgtt acaaaacatt tttcggccgc aagtgtaaag
    16261 tttttgtgtt ctgcaactgc aagcacatat tgaagctgtg taatagtcat aataagtata
    16321 tttgatgcaa atataaaaac tatcaattta agttatagac ttagtttaac taattattta
    16381 caaaacttag atgtattatt taaaaaaaac tacttgtatt aaatttagga ttaggattca
    16441 tagttttaaa taattgttaa attcgatata tactttagga aatttttgtg ttaaagttta
    16501 tttttgccaa atgaagttat tcaccaggat tttattattc ctatttattc tttttctttc
    16561 aacaccaact ttggttactc taattgaaaa agattcggat atttctatgt tttattgttt
    16621 tgctgaagaa gagcatcaca aagaaataaa agcagaaatt aaattttatg tagagcataa
    16681 tgtaattgca tttatggaac tagaaaaaaa gacttctttc attgagaata actcagaaca
    16741 cccaaccgta tttagagaaa tattttctcc accacctaat tttatataaa tcaattttac
    16801 atttcttaat tgaaatgaaa catttttata taacttttaa aattaggtat gacaaaaaaa
    16861 attaatcttt ttgctaacct caaatcagat tttgcatctg gtttagtggt ttttttggtg
    16921 gcattgccac tgtgtttagg tattgcactt gctagtggtg cgccattgtt ttcaggaatt
    16981 atagcaggaa ttgtgggcgg aatagtagtt ggctatttga gtaaatcgca tctcagcgtt
    17041 tctggtccgg ctgctggttt aacggctata gttttagtag ctattactga tttaggttct
    17101 tttaatgcct ttttactggc gggtttaata gctggtggta ttcaattatt gcttgggttt
    17161 attaaagcgg gtagtatttc caattatttt tcaactaatg ttattgaagg aatgttagcg
    17221 ggtattggag ttatcatatt tttaaagcaa attcctcacg cttttgggta tgataaggat
    17281 tatgaaggtg atttggcatt taaccaacct gatggtcaaa atacttttac agaactattt
    17341 tcggttcttg gtcatgtaca acttggtgca ttactaataa caatagtttc tttattcata
    17401 ttaattgttt gggataaaat acctgcatta aagaaattaa aactagttcc ttctgcttta
    17461 gttgcagtat ttgttggggt attaattaat gagtatttta ttcagtcggg aagtagcttg
    17521 gctgttttag acaatcatct tgttaaatta ccagtaccaa catcattaga agaattaggc
    17581 gcaataatag taacgccaga tttttcggca attacaaact ctaaagtatg gattgttgga
    17641 atcacaatag ctgtggttgc atcaattgaa acgttacttt gtatcgaagc tgccgataga
    17701 atggatgagc aaaaaagata tacagatacc aatgttgagt taaaagcaca aggtattgga
    17761 aacattatta gttctttact tggtggttta ccaatgacat ctgttgtagt aagaacaact
    17821 gccaataata ctgctggtgc aaaatcaaaa atgtctgcaa ttatacatgg tgttttactt
    17881 ttaattagcg tattggctat tccggtttta ttaaataaaa ttccgttagc tactttagca
    17941 gcaatacttc tattgatagg ttataaattg gcaaatccta gtaaaataaa gcatttttgg
    18001 gaaaaaggaa aatatcaatt tataccattt attgcaacag ttatagctgt agtatttaca
    18061 gatttattaa aaggagttgc attaggtatc ataattagta ttatttttat tatgaaggga
    18121 aatttacaac gtgcctataa attcagaaaa gaagaatata aagatggaga cattattcac
    18181 attgatttgg ctcaagaagt ttcattcttg aacaaagctg ctatcaaaca aacgttatct
    18241 tcaattcctg aaaattctaa agtgataatc aatgcttcag atacggtata tattgctcat
    18301 gatgttttag atttaattaa agaatttaaa aaaataaggg ctaaagatga aaatataaaa
    18361 gtgaagttag aaggtttcaa aaaagcctat aatttaattg aaacgggcga ggaaggaaaa
    18421 cacgtattta ttgaacataa gtaatcatta tgaggattat atacgtctta ataacaattt
    18481 tttgtttgaa tagtttagca attgcccaaa aatcagctat tggaaattgg tttctttatt
    18541 ttgggaatca aaagataaat gaaaagtgga attggcataa cgaaattcaa tacagaaatt
    18601 acaattttat tggcgatacg aatcagttac ttctgcgaac cggaatagga tacaacctta
    18661 cggaaaacaa caataatctt cttttgggtt atggttatat aaatacacaa aattatatac
    18721 cagatactga tgaaaaagta catataaatg aacatcgaat ttatcagcag ttcatcacaa
    18781 aggagcactt tggtagattc tttcttcaac atcggtatcg aattgaagaa cgatttgtag
    18841 ctaatgagtt t
//
LOCUS       DAOOBH010000033        18837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_3888_length_18837_cov_6.511341, whole genome shotgun sequence.
ACCESSION   DAOOBH010000033 DAOOBH010000000
VERSION     DAOOBH010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 18837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18837
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3888_length_18837_cov_6.511341"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK218_05275"
     CDS             <1..196
                     /locus_tag="PK218_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HPW97952.1"
                     /translation="HEFYTIISHSFSTLQKGNIQLNYVFPIHGHLRGQAQVFQGYGET
                     LIDYNVSQTTFGIGVSFANW"
     gene            complement(227..802)
                     /locus_tag="PK218_05280"
     CDS             complement(227..802)
                     /locus_tag="PK218_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPW97953.1"
                     /translation="MALILENISKIVSLSTEEENLFLTKVEIKRYKHKTVLLNAGEIC
                     KHSYFVNSGILRSFTINDHIVEHVLHFACEGWWIGDMYSLLSQKPGNLFIEVLEDAEV
                     IQLSKENQDELYEKIPKLERFFRILTENSLVAHQERLMDSLSLTAEERFEKFYKKYPT
                     LLQKVPQKHIASYIGVTPEFYSKMKSKLLRS"
     gene            complement(803..1117)
                     /locus_tag="PK218_05285"
     CDS             complement(803..1117)
                     /locus_tag="PK218_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HPW97954.1"
                     /translation="MKNVFSLVILFVSITISAQEKPKVQIVEASCGQCQFGMKGKKGC
                     DLAVRIDGKSYFVDGTKLDDHGDAHAEDGFCSAVRKAEVVGEVKDNKFVVSYFKLLPI
                     KE"
     gene            complement(1121..2284)
                     /gene="purT"
                     /locus_tag="PK218_05290"
     CDS             complement(1121..2284)
                     /gene="purT"
                     /locus_tag="PK218_05290"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HPW97955.1"
                     /translation="MAKILLLGSGELGKEFAIAAQRIGQYIIAIDSYENAPAMQVAHE
                     FEVINMLDGSELDRIVGKHQPDFIVPEIEAIRTERFYEYEKQGITVVPSAKAANFTMN
                     RKAIRDLAAKDLGLRTARYRYATSADELQKAVREVGIPCVVKPLMSSSGKGQSTIKSV
                     EDISKAWNYAVEGSRGDVIEIIVEAFVDFHSEITLLTVTQNGNPTLFCAPIGHRQERG
                     DYMESWQPARVSKKDLAEAQDMARKVTEALGGAGLFGVEFFLTNEGVYFSELSPRPHD
                     TGMVTLAGTQNFNEFELHLRTILSLPIFEITLEKNGASAVVLASENSNNPTYTGIEKV
                     AALPKTDFRIFGKPTSRPYRRMGVVLTHDTLQTPIEEIVEKAKVNSKLIIVNP"
     gene            complement(2359..3546)
                     /locus_tag="PK218_05295"
     CDS             complement(2359..3546)
                     /locus_tag="PK218_05295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPW97956.1"
                     /translation="MNHLLSDRVNNLSTSQTLAMAALARELKAQGKDIISLSLGEPDF
                     NTPDFIKEAAKKAIDENYSTYSPVDGYVELKEAICRKFKRDNNLEYKPSQIVVSTGAK
                     QALYNIAQVMLNDGDEVILPAPYWVSYYEIIKLSGGTPIEVPTSVETDFKMTAEQLEK
                     AITPKTKMMWFSSPCNPSGSVYNREELTALCKVLEKHPNIYVVSDEIYEHINFSGTFC
                     SIASIPGMLEKTVTVNGVAKAFAMTGWRIGYIGAPEFIAKACTKIQGQVTSGANSIAQ
                     RATITAVDADPSVLNEMVAAFKSRRDLVVGFIQNIPGLKINIPEGAFYVFPDVSSFFG
                     KTLKGTKIENATDLSMYLLAEANVATVTGDAFGNPNCLRISYATSETILTEALKRIKE
                     ALA"
     gene            complement(3715..4809)
                     /locus_tag="PK218_05300"
     CDS             complement(3715..4809)
                     /locus_tag="PK218_05300"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HPW97957.1"
                     /translation="MNTTTPIFSKTDSLKFFRTLNKRVNDYFKENKIDKTGNWKLHLK
                     TIVMFSIFLTPYFFLLAIDMPFWTYLLLSVVIGIGMAGVGMNVMHDGNHGAYSSKSWV
                     NKFMGGSIYILAGNVYNWQVQHNVLHHTYTNILGHDEDLEAGRIMRFTQEAKWYRFHK
                     FQHFYSVFLYGLLTFNWSITTDFLQMKRYLKRNLSYGEWKKPSIRWTTLIITKVIYFS
                     IWIVIPMLLGIIWWKVIIGFLVMHYTAGVILSVVFQLAHVVEDTHNPIPDDNGEIENT
                     WAIHQLFTTANFAPKNWLVNYYTGGLNHQVEHHLFPNISHIHYGKIAEIVKQTAQECN
                     LPYFEFKTTREAIYSHFKHLKELGQKPQLA"
     gene            4919..5548
                     /gene="rsmG"
                     /locus_tag="PK218_05305"
     CDS             4919..5548
                     /gene="rsmG"
                     /locus_tag="PK218_05305"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPW97958.1"
                     /translation="MEEILKQFPNLTENQIQQFHKLETLYQDWNAKINVISRKDIDEL
                     YTKHVLHSLAIAKIQPFVAGSYVLDVGTGGGFPGIPLAILFPETRFYLIDVIAKKIKV
                     VQAIAEALELKNVKAEQLRAENVKGDFDFIVSRAVTNMPDFVSWIKDKIKKQNKHELK
                     NGILYLKGGDLTKELASFPKATEYNIADFFSDEFFETKKVVHLPLKFKA"
     gene            complement(5583..7859)
                     /locus_tag="PK218_05310"
     CDS             complement(5583..7859)
                     /locus_tag="PK218_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW97959.1"
                     /translation="MKLKITLLALLSGFISTFAQDKMFDLLKDKTETNILYNRVFALS
                     HANEMKTKEISTTYFLQVYHEIQRADFNNRLPKLEVIRKAATLASIKNQIPLSVLISD
                     FESISENDLQNKFNLNASNQLELKNNETLELEKHAINIVAPIVAKSKTNEVTFVLNSD
                     LIFNTTSREIKSIFYSKENSSWQKINQNQPFTVHFAANGTQELALKIEFTNGTSTEES
                     ISFEVNSTILIHRNSNSVMAASSITATIPYQGFGETAAVLGQGEFEIFLDNVDGVLDK
                     PIILVDGFDPGDTRNTTTIYDLLNYGTNQNFADDLRNQGFDIVVLNFPTYTIPGTTTV
                     IDGGADYIQRNAYILVELLNQINAQKVGAEKNVVIGPSMGGLISRYALRYMEMNNLVH
                     DTRLYLSFDSPHQGANVPIGFQHLFNYMAYGPLGNAAVQPIVDGLIKSPAARQMLIDH
                     MEGHLQSGSAFEFNTAAASLLPTGAPNFRTAFQDELNTMGFPTLTRNVSIANGAGNGT
                     MNYTPDFEVMNHTFNITTSQRAIINLRFTPAANQTNQVSRFRGQQYVVILWITGFESL
                     ANSKAPTFTAGLDSAPGGRFDMTGFAGGLGTDPILTEFFDNLNADYFTFIPTWSSMAI
                     SGTNNLYTPVTASTVSPFVASSIPTINENHITFNAQNVAFALNEILNPPLSTSNPELE
                     SIWVKNPVENVIEINANYSLENASILLTDMLGKNIYQSNNQTIHGSLSIPIDVSKGIY
                     LLTIDNGKQRVTKKLVKN"
     gene            complement(7949..8710)
                     /locus_tag="PK218_05315"
     CDS             complement(7949..8710)
                     /locus_tag="PK218_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPW97960.1"
                     /translation="MLYSKIEGEGKPLIILHGFLGMLDNWKSIGSKYVENGFQVHLLD
                     MRNHGRSFHSEEFNYRVMANDIFEYCQNHNLVKIDLIGHSMGGKIAMLFASTYPKLVD
                     KLIIADIGPKYYKPHHQDILAGLNAVDFSTKPDRSEVEEVLAHYIPDFGTRQFLMKSL
                     FWREQGQLAFRFNLEVFNDKIEEVGKELPIEKHFENPTLFIRGGNSRYILDSDLPDIK
                     RQFPNFELKTIPNVGHWLHAENPELFLKITLDFLV"
     gene            complement(8757..9470)
                     /locus_tag="PK218_05320"
     CDS             complement(8757..9470)
                     /locus_tag="PK218_05320"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPW97961.1"
                     /translation="MTKLSVNINKIATLRNARGGNVPDLLKVATDIQKFGAQGITIHP
                     RPDERHIRYQDARDLKSIVTTEFNIEGNPQHNFIDLVLECKPEQVTLVPDAIGAITSS
                     AGWDTQKNQSYLTEVIQEFQRKGIRTSIFVDPILEMIEGAKKTGTDRIELYTEEFAKQ
                     YDLGNKTGIEPYIQAAILANELHLGINAGHDLSLENIKFFKDNIPNLLEVSIGHALVS
                     EALYLGLDNVVNMYLQKLK"
     gene            9583..10242
                     /locus_tag="PK218_05325"
     CDS             9583..10242
                     /locus_tag="PK218_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein acuB"
                     /protein_id="HPW97962.1"
                     /translation="MRILSEYINNDFKPFLISENVSEIQDFFAENTFSHFPVVDNQVF
                     LGCISANDSETFEFGKTLADYRYAFEGFFVREPMIWLDVLEIFARNNANIVPILSESN
                     QYVGYYEITDVIKFLNETPFLKETGGIIVVEKLTVDYSMSQIAQIVESNNGKLLGLFV
                     SEASNDKVQVTVKTTLVGLNEIIQTFRRYNYEIISEHNEDDYLNNLKERSEYLDKYLN
                     I"
     gene            10245..11126
                     /locus_tag="PK218_05330"
     CDS             10245..11126
                     /locus_tag="PK218_05330"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HPW97963.1"
                     /translation="MKVAIFGQYYQNDTRPIIKDIFVFFNRNEVELVIEEKFLKILYE
                     EKIIEKEYNTFKSGTELDQSFDILISIGGDGTILRAATYVGNSGIPILGVNAGRLGFL
                     AKVQKEDIQSFLQIVLEKKYTISERTLLKLETNPKNDEIEIDFAMNEISVSRKDTTSM
                     ITIETSLNGEHLNSYWADGLIISTPTGSTGYSLSCGGPILTPDVKGFVITPIAPHNLN
                     ARPLVIPDNTEIKLKVSGREEQYLVSLDSRILSINNNSELFIRKNHFSINMVEFPNES
                     FFKTLRKKLLWGEDKRN"
     gene            11290..11976
                     /locus_tag="PK218_05335"
     CDS             11290..11976
                     /locus_tag="PK218_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPW97964.1"
                     /translation="MKKNLVLFFLMISTHFLNAQIHEIGVFLGGSNYIGDVGKTNYIN
                     PNKIAFGILYKWNKSPRHSYRFSYTQSSIIANDANSKILGRELRGYQFKNSIKEFSAG
                     LEFNFFDFNLHEALKMKTTPYVYSGVSYFIYDELYVLNGVTKNDYQRGEFAIPMIVGV
                     KSKLTETLIIGFEVGARYTFTDNLDGSLPKNGNLQSLKFGNINSNDWYVFTGFTLTYT
                     FGNKPCYCAE"
     gene            11978..12715
                     /locus_tag="PK218_05340"
     CDS             11978..12715
                     /locus_tag="PK218_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPW97965.1"
                     /translation="MSIENIDTKKLPKHLAIIMDGNGRWAKQKGLLRALGHENGTKSV
                     KATVESCAKLGIENLTLYAFSTENWNRPKLEVDTLMKLLIASLKKELETLQKNNIQLN
                     SIGNIDLLPSKAKKELEAVIEKTKNNTRMTLTLALSYGAREELLNAVKKISDKVKNNI
                     ISLDSIDESIINQHLYTHNLPDVDLVIRTSGEHRISNFLLWQIAYAEFYFTDVLWPDF
                     KEDDLYQAIISYQKRERRFGKTSEQIK"
     gene            12699..15401
                     /gene="bamA"
                     /locus_tag="PK218_05345"
     CDS             12699..15401
                     /gene="bamA"
                     /locus_tag="PK218_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPW97966.1"
                     /translation="MNKLNRILVQINTLKIVLTVLLVGTVFQLQAQDRIPFDQGRKYI
                     LADVEVVGKISYNQQTVVTFSGLEKGQEITVPGEQISNAIKKLGKLGLFSDIDFYINK
                     TQNDSIWLELAISELPKLSEVKIQGVKKSKVESLIKDNSLTKGKIVNENLITTTKNYI
                     ENKYKKDGYYNTKVFIKTIADTTEGNQVKMLVNIDKGDKLKIARIDFDGNEKFSDKKL
                     RGAMKNTKKQNFIRIFKASKFVKDKYKEDLVSIVDKYKEKGYRDARIISDSVYLNEKK
                     NKININIKLEEGRKYYFGDIKFLGNSVYSDQLLSRVLGIKKGETYNGVLLQKRIADKT
                     KPDAEDLTNLYQNNGYLFSNINAVEVKTENDTIDFEIRVVEGPIAYFNNITVVGNDKT
                     NDHVIYRELRTRPGEKYSKEDLVRTIREIGQLGFFDPESIDPKFKNVDPASGTVDIEY
                     NLVEKGSSQIELQGGYGGGGFIGTLGLSFNNFSVRNMFKKDAYKPLPMGDGQKVSLRA
                     QASSFFQTYSLSFSEPWFGGKKPIQFSSSLSHSKQFLYSGNSASVDKTRSFNITSLSV
                     GISKRLTVPDDNFYFSNAVSFQYYDLNNYNTGLFTFGDGSSRNLAFTLGLTRNNKGIN
                     PIYPTSGSEFSVSAKFTLPYSLFNGIDYGNLGNLIENKLTYDPSKGLNVQPTSQGQVP
                     SIGDYLGVAQTVNGQTIYQVVDTYQEAAADQSKVDQLKFNWLEYYKLKFKADWYTKVA
                     GSQQKALVLRALGEFGYLGAYNNARGVVPFERFFLGGDGLANFALDGREVVQLRGYPN
                     QSLSSVDGGTIYNKFTLELRYPLTLKAAASIYALTFLEAGSSFDSFQEYNPFVLQRSA
                     GFGLRVFMPAFGLLGIDFGHGFDPLPGGTQKNGWETHFIIGQQF"
     gene            15435..16403
                     /locus_tag="PK218_05350"
     CDS             15435..16403
                     /locus_tag="PK218_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPW97967.1"
                     /translation="MKKYIAILIILLTSTQLNAQSRGVKIGYIDMEYILQNVTDYSEA
                     KNQLEQKAQKWKQDIEAKKVEIAKLKDELKTEKPLLTKELIDEREEEIAFQEQELLDF
                     QQKRFGPSGDLITQKAVLVKPIQDQVFNAVQDIAEQQKYDFIFDKSSDLTMLFAAKRY
                     DISDRVLRVLNRSEKRQQMSAKQLKEQEKKEALEDMEDENPAIAERRKKLEERKAARD
                     KLLEDRKAAAEAKKAEQQAKRDAAAAEREAKKNGTVNADEKPSSENTTEKDSTATTTK
                     EDKAAERARILAERKKELDDKRKKILEEREAAKKAREEKNNEPKTN"
     gene            16437..16937
                     /locus_tag="PK218_05355"
     CDS             16437..16937
                     /locus_tag="PK218_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPW97968.1"
                     /translation="MKRLKSLLIAGVLFFGTMQTINAQAKTAHVDVNEIISKMPAMLD
                     SQKQLEKLSATYDAEYKTMAEEYQAKIKKYDQEASTVTDAVNQTRQQEVQDLVKRITD
                     YRDNAQKELQKKESDLVKPLMDKIRASIAKVGKAKGYQYVLNAADLLLTDGPDLTADV
                     KKDLGF"
     gene            17003..17782
                     /gene="murI"
                     /locus_tag="PK218_05360"
     CDS             17003..17782
                     /gene="murI"
                     /locus_tag="PK218_05360"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPW97969.1"
                     /translation="MINNNPIGLFDSGIGGTSIWKEIHDLLPNENTIYLADSKNAPYG
                     QKSKEEIVQLSIKNTEFLLNQNCKIIVVACNTATTNAIKELRAKYDVPFIGIEPAIKP
                     AALNSSTNTIGILATKGTLNSELFHQTLEKFQETKIIEQIGHGLVTLIENGELNSIET
                     KNLLKTYLKPMIEANIDYLVLGCSHYPYLIPQIKKIIPTKIKIIDSGEAVARQTKNVL
                     VEKSILNLENKLGKAVFYTNANPKVLENILKNKEEVIQKDF"
     gene            complement(17840..18364)
                     /locus_tag="PK218_05365"
     CDS             complement(17840..18364)
                     /locus_tag="PK218_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPW97970.1"
                     /translation="MILKTVNGKKPQIPEDCYVAENATIVGDVTFGHSCSVWFNAVIR
                     GDVHFIKIGNKVNIQDGAIIHCTYQKHPTEIGNNVSIGHNAIVHGCKIHDNVLIGMGA
                     IVMDNCVVESNSIVAAGAVVTQNTVIESGSIYAGVPARKVKEINQSDFGGEIERISNN
                     YVMYSGWFKNENEK"
     gene            complement(18409..>18837)
                     /locus_tag="PK218_05370"
     CDS             complement(18409..>18837)
                     /locus_tag="PK218_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPW97971.1"
                     /translation="DREPVNLRRFILNSGYTFGDADKILWEPSFMFQLVTQTQEKTID
                     LNVKAYKNLDFGMLWGGISYRRSLDGAQYVEGQSIGDQKLQYITPILGVNFKNYMLSY
                     TYSQLLGNVKFDSAGFHQLTLGINLFCKPEKYHCNCPAIN"
BASE COUNT         6589 a         3129 c         3002 g         6117 t
ORIGIN      
        1 acatgagttt tacaccatta tatctcattc gttttcgaca cttcaaaaag gaaatattca
       61 attgaattat gtgtttccga tacatggcca tttgagaggt caagcgcaag tatttcaagg
      121 ctatggtgaa actttaattg attataatgt gagtcaaaca acttttggaa ttggtgtttc
      181 ttttgctaat tggtaattat taattgaata aaatgatatt taagcatcaa ctccgaagca
      241 gtttactttt cattttacta taaaactcag gagtaacgcc aatataggaa gcaatgtgtt
      301 tttgcggtac tttttgaagt aatgttggat attttttata aaatttttca aatctttctt
      361 cggcagttaa acttaaactg tccatcaatc gttcttgatg agctacgagt gagttttcag
      421 ttagtatcct aaaaaagcgt tccaactttg ggattttttc atacaactca tcttgatttt
      481 ctttggacaa ctgaataact tcggcatctt ccaaaacttc gatgaaaaga tttcctggtt
      541 tttgtgaaag caaactatac atatcgccaa tccaccaacc ttcgcatgca aaatgtaaaa
      601 cgtgttctac aatatgatca ttaatagtga aacttcttag aataccagaa ttgacgaaat
      661 aggaatgttt acaaatttcc ccagcattta aaagtactgt tttatgtttg taccttttta
      721 tttcaacttt tgttaaaaac aaattttctt cttctgtgga aagagaaact atttttgaga
      781 tattttcaag aattaatgcc aattattctt ttattggaag taatttaaaa taggaaacaa
      841 caaacttatt gtcttttact tcaccaacaa cttctgcttt tcttactgct gaacaaaatc
      901 catcctctgc atgtgcatct ccatgatcgt ctaactttgt tccatcaaca aaataggatt
      961 tcccgtctat ccgaacggct aaatcacagc cttttttgcc tttcatccca aactgacatt
     1021 gaccacaaga cgcttctacg atttgaactt ttggtttttc ttgtgcgcta attgtaatcg
     1081 aaacaaaaag gattactaat gagaatacat ttttcataat ttacggattt actattatta
     1141 attttgaatt gactttagct ttctctacaa tttcttcaat tggagtttgc aatgtgtcat
     1201 gggttaatac aacacccatt cttctgtatg gtcgcgaagt tggttttcca aagatacgaa
     1261 aatcagtttt aggtaaagca gctacttttt caattccagt ataggttgga ttatttgagt
     1321 tttcagatgc taatacaact gcacttgcac catttttctc taaagttatt tcgaagatag
     1381 gtaaacttaa aatggttcga aggtgcaatt caaattcgtt gaagttttgt gttcctgcta
     1441 atgttaccat tccagtatcg tgtggtcgag gcgataattc ggagaaataa acaccttcat
     1501 tagttaagaa aaactccacg ccaaaaagac cagcaccacc aagtgcttcg gtaacttttc
     1561 ttgccatatc ttgtgcttct gctaaatctt ttttagaaac tctagcgggt tgccaacttt
     1621 ccatataatc accacgctct tgtctgtgac caattggtgc gcaaaataaa gttgggtttc
     1681 cattttgtgt tacggttaat aatgttattt ctgaatgaaa atcaacaaaa gcttccacaa
     1741 ttatttcaat tacatcgcct cgtgaacctt caactgcata attccaagct tttgaaatat
     1801 cttctacaga ttttatagtt gattgtcctt ttccagacga actcattaat ggttttacaa
     1861 cgcatggtat cccaacttct cgaactgctt tttgtaattc gtctgctgag gttgcatagc
     1921 ggtaacgggc agttcgtagc cctaaatctt tagccgccaa atcacgaatt gcttttctat
     1981 tcatagtaaa gttggcagct tttgcagaag gaacaacagt tataccttgt ttttcatatt
     2041 cgtaaaaacg ctccgtgcga atagcttcaa tttctggaac aataaaatca ggttgatgtt
     2101 tacctacaat tctatctagt tcagaaccgt caagcatatt gatgacttca aattcgtgtg
     2161 ctacttgcat cgctggagca ttttcataac tgtcaatagc tatgatatat tgaccaattc
     2221 tttgtgccgc aattgcaaat tcttttccta attcgcccga acctagtagt agtatttttg
     2281 ccattgtatt tgtttcaagt taaggtttca agttccaagt tttaaacctg aaacttgaaa
     2341 cctgaaacaa atttttattt atgctaacgc ttcttttatt cttttcaaag cttcagtcaa
     2401 aatagtttca ctagtggcat aagaaattcg caaacaattt ggatttccaa aagcatcgcc
     2461 agtaaccgtt gctacatttg cttctgccag taaatacatt gataaatcgg ttgcgttttc
     2521 aatttttgtt ccttttagcg tttttccaaa gaaagaagaa acatctggaa aaacataaaa
     2581 tgcaccttct ggaatattaa tttttaaacc aggaatattt tggataaaac ctactactaa
     2641 atctcttcta cttttaaagg cagcaaccat ttcgtttagt acacttggat cagcatcaac
     2701 tgccgttata gttgctcgtt gtgcaataga atttgctcca gaagtaactt gtccttgaat
     2761 tttagtgcat gcttttgcta taaattcggg tgcgccaatg tatccgattc tccaacccgt
     2821 catggcaaaa gctttggcaa cgccattgac agtaaccgtc ttttcaagca ttccaggaat
     2881 ggaagcaatg ctacaaaatg ttcccgaaaa attaatatgt tcgtagattt catctgaaac
     2941 gacatagatg tttggatgtt tttctaaaac tttgcacaaa gctgttaatt cttcacggtt
     3001 atatactgaa ccactaggat tacaaggcga gctaaaccac atcattttcg tttttggtgt
     3061 aattgctttt tctagttgtt cggctgtcat tttgaaatct gtttctacag aagttggaac
     3121 ttcaattgga gttccaccag atagcttgat gatttcataa taagaaaccc aataaggtgc
     3181 tggaagtata acttcgtcac catcatttaa cattacttgt gcaatgttgt ataaggcttg
     3241 ttttgctcca gtagaaacaa caatctgtga tggtttgtat tctaggttgt tatctctttt
     3301 gaattttcta catatggctt ctttcaattc tacataacca tcaactggcg aataggtaga
     3361 atagttttca tcaatagctt ttttggctgc ttctttgatg aagtctgggg tattaaaatc
     3421 tggttcgccc aaacttaaac tgatgatatc ttttccttgg gcttttaatt ctcttgctaa
     3481 tgctgccatt gcaagtgttt gtgatgttga gagattatta actctgtctg ataacaaatg
     3541 gttcatgtgg gtgtttttgt gttgattagt gcgtagcgat ttagtacttt gttgaattag
     3601 tactcagtac ctagtggatt agtatatagg ccttagttga ttagttctta gtgttttgta
     3661 ctacttacta tgtgctacca actatgtact aaggactact tacaaaacaa ctaattaagc
     3721 tagttgaggt ttttgaccta attcttttaa atgtttgaaa tgcgaataga tagcttcgcg
     3781 agttgtctta aattcaaaat aaggcaaatt acattcttga gccgtttgct tcacgatttc
     3841 agcaatttta ccataatgaa tgtgactgat atttggaaac aaatgatgtt ctacttgatg
     3901 atttaatcca ccagtataat aatttactag ccagtttttg ggtgcaaaat ttgctgttgt
     3961 aaacaattga tgaattgccc atgtattttc aatttctccg ttatcatctg gaattggatt
     4021 gtgcgtgtct tctactacgt gtgccaattg gaatacaaca ctcaaaatta caccagctgt
     4081 gtaatgcatt actaaaaatc caataatcac tttccaccaa atgataccta aaagcatggg
     4141 aataacaatc catatagaaa aataaataac ttttgtaatg attaaagttg tccatcgaat
     4201 agaaggtttt ttccattcgc cataagaaag atttctttta agatagcgtt tcatttgaag
     4261 aaaatcggtt gtaatagacc aattaaaagt caataaacca tataaaaaaa cagaataaaa
     4321 atgttggaac ttgtgaaaac gataccattt tgcttcttgt gtaaatcgca ttattcgtcc
     4381 tgcttctaaa tcttcgtcat gaccaagaat attggtgtaa gtatgatgca aaacattgtg
     4441 ttgcacttgc cagttgtaaa catttcctgc taaaatatag atgcttccac ccataaattt
     4501 gtttacccat gatttgctag aataagcacc atgatttccg tcgtgcataa cgttcatacc
     4561 aacgccagcc attcctattc cgatgacaac gcttagtagt aaataggtcc aaaaaggcat
     4621 gtctattgct aaaaggaaaa aataaggtgt taaaaaaatg gagaacatca caatagtctt
     4681 taagtgtaat ttccagtttc ccgttttatc aattttattt tctttaaaat aatcgttaac
     4741 tcttttgttt aaagttctaa aaaacttaag gctatctgtt ttagaaaata ttggtgttgt
     4801 ggtattcatt tatttttatt ctaaatttgg tgtaaagata attattaaaa ttttttaaaa
     4861 gatgaaaaga aatcataatt attaaccaaa aaccttattt ttgttgaaaa gctaaaacat
     4921 ggaagaaatt cttaaacaat ttcctaatct tactgaaaat caaatacaac agtttcacaa
     4981 attagaaacc ttatatcaag attggaatgc aaaaattaac gtaatttcta gaaaagatat
     5041 tgacgaactc tataccaagc atgtgttaca ctcattagca attgctaaaa ttcaaccttt
     5101 tgttgccgga agttatgtgt tagatgttgg aactggcggt ggttttccag gaattccgtt
     5161 agcaatcttg tttcccgaaa cacgttttta tttaattgat gttattgcca aaaaaataaa
     5221 agtagttcaa gccatagccg aagcattgga acttaaaaac gtaaaagccg aacaactacg
     5281 cgcagaaaat gtaaaaggtg atttcgactt catcgtttcg cgtgccgtaa ccaatatgcc
     5341 cgattttgtt tcttggatta aagataaaat caaaaagcaa aacaaacacg aacttaaaaa
     5401 cggaattctt tatctaaaag gtggcgattt aaccaaagaa ctcgcctctt ttccaaaagc
     5461 tacagaatat aacattgcag actttttttc agatgaattt tttgaaacca agaaagtagt
     5521 gcatttgcct ttgaagttta aagcataaaa aaagcgccta atactagacg cttttaattc
     5581 ttttaattct tcactaattt cttcgtgaca cgttgctttc cattgtcaat cgttaacaaa
     5641 taaatccctt ttgacacatc aattggaatt gataaacttc catggatagt ttgattattc
     5701 gattggtaaa tatttttccc taacatatcg gtcaataaaa ttgatgcatt ttcaagagaa
     5761 taattagcat ttatttcaat aacattctcc acaggatttt ttacccaaat ggattccaat
     5821 tcaggattac tagtacttaa aggtgggttt aaaatttcat tcaaggcaaa agctacattt
     5881 tgagcgttaa acgtaatatg gttttcatta atagtaggaa tagacgaagc tacaaaagga
     5941 ctaacagtgc ttgcagtaac tggagtatat aaattgttag ttccagaaat cgccatcgaa
     6001 ctccaagttg gaataaacgt aaaatagtca gcattcaaat tatcaaaaaa ctccgttaaa
     6061 attgggtcag ttcctaagcc gccagcaaat ccagtcatat caaaccttcc accaggtgcc
     6121 gaatctagtc ctgctgtaaa ggttggcgct tttgaattgg ctaaactttc aaaacctgtt
     6181 atccaaagta taaccacata ttgttgacct ctaaatcggc ttacttgatt ggtttgattg
     6241 gctgcaggag taaatcgtaa attaataatc gctctttgag aagtggtgat attaaaagta
     6301 tgattcatca cttcaaaatc cggagtataa ttcatcgttc catttccagc accgtttgca
     6361 atcgaaacat ttcttgtaag tgttggaaaa cccatggtat tcaattcatc ttgaaaagct
     6421 gttctaaaat tcggtgcgcc tgttggcaat aaagatgctg cggcggtatt aaattcaaaa
     6481 gcacttccac tttgcaaatg tccttccata tgatctataa gcatttgtcg tgccgccgga
     6541 ctcttaatca atccatctac aataggttgc acagcagcat ttcctaatgg cccataagcc
     6601 atataattaa acaaatgctg gaagccaatc ggtacatttg ctccttgatg tggcgaatca
     6661 aaagagaggt ataaacgagt atcatgaact aaattattca tttccatata gcgcaatgca
     6721 tatctcgaaa ttaatccgcc catactagga ccaataacca catttttctc ggcacctact
     6781 ttttgagcat taatttgatt taacaattct actaaaatgt acgcgttcct ctgaatataa
     6841 tcagcaccgc catcaataac agtagttgtt cctggtattg tataggttgg aaaatttaac
     6901 acaacaatgt caaaaccttg atttcttaaa tcatcagcaa agttttgatt ggttccataa
     6961 ttcaataaat cataaatagt agtagtgttt ctagtatcac caggatcaaa accatccaca
     7021 agaataatcg gtttgtctaa aacaccatcc acattgtcta agaaaatttc aaattcacct
     7081 tgtccaagaa ccgctgccgt ttctccaaat ccttggtaag gaatcgttgc cgttattgaa
     7141 gaagcagcca ttaccgaatt tgaatttcta tgaatcaaaa tagtactatt cacttcaaaa
     7201 gatattgatt cttcagttga agttccgttt gtaaattcaa tttttaatgc caactcttgt
     7261 gttccattag ctgcaaagtg aacagtaaaa ggttggtttt gatttatttt ttgccaagaa
     7321 ctattttctt ttgaataaaa aatcgattta atttctcttg aagtcgtatt aaaaatcaaa
     7381 tcgctgttta aaacaaaagt tacttcattt gttttcgatt ttgcaacaat tggtgcaacg
     7441 atattaatag catgtttttc aagttcaagc gtttcgttgt tttttaattc taattggttc
     7501 gaagcattta agttaaattt attttgcaaa tcattttcag aaatagactc gaagtcagag
     7561 attaaaacag atagcggtat ctggtttttg atgctagcca gtgttgccgc ttttctaatg
     7621 acttccagtt ttggcaaacg attgttaaaa tcggcacgtt gaatttcgtg atacacttga
     7681 agaaagtaag tagtgctaat ttccttagtt ttcatttcgt tggcatgcga aagcgcaaaa
     7741 acacggttgt ataaaatatt cgtttctgtt ttatctttca ataaatcgaa cattttgtct
     7801 tgagcaaaag tgctaatgaa gccacttaat aatgcaagca gtgtaatttt caatttcata
     7861 gtaatatggt ttattgggtt ttaacgctcg aagatacaaa aaaataatat tgatatacat
     7921 aaaaaataac cttttatcga aaggtttttt aaacaagaaa gtctaaagtt atcttaagaa
     7981 aaagctcagg attctctgcg tgcaaccaat gcccaacatt tggaatggtt tttaattcaa
     8041 aattgggaaa ttgtctttta atgtctggca aatcggaatc taaaatatat ctcgaattac
     8101 cgccacggat aaataatgtt gggttttcaa aatgtttttc gattggtaat tcttttccaa
     8161 cttcttcaat tttatcatta aaaacctcta gattgaacct aaaagccaat tgtccttgtt
     8221 ctctccaaaa aaggcttttc attagaaatt gccttgtccc aaaatcagga atatagtgag
     8281 ccaaaacctc ttcaacttcg ctcctatcgg gctttgttga aaaatcgact gcatttaaac
     8341 ctgctaaaat atcctgatga tgaggtttgt aatatttggg accaatatcg gcaatgatta
     8401 atttatcaac taattttggg taagtacttg caaaaagcat ggctattttt ccacccattg
     8461 aatgcccaat taaatcaatt ttaacaaggt tatggttttg acaatattca aaaatatcat
     8521 ttgccattac cctataattg aattcctccg aatgaaaact tctaccatgg tttcgcatat
     8581 ctaacaaatg tacttgaaaa ccattttcaa catacttcga accaattgat ttccaattgt
     8641 ccaacattcc taaaaatcca tgtaagatta taagtggttt tccttcgcct tctatttttg
     8701 agtacagcat cttatataat agatattaat ttttgatttt aaattgactg tgtcatttat
     8761 ttcagttttt gcaaatacat atttactaca ttatccaatc caagatacaa agcctctgaa
     8821 actaaagcat gaccaattga aacttcaagt aagtttggaa tattatcttt aaaaaacttg
     8881 atattttcca aactcaaatc gtgaccagca tttattccta agtgcaattc atttgccaga
     8941 attgcagctt gtatataggg ttcaatacct gttttatttc ctaaatcata ttgttttgca
     9001 aattcttcgg tatataactc gattctatct gttccagtct tttttgctcc ctcaatcatt
     9061 tctaaaatag gatctacaaa aatagaagtt ctgattcctt ttctctgaaa ctcttggatt
     9121 acttctgtca aataggattg gtttttttga gtatcccaac cagcagaaga agttattgct
     9181 ccgattgcat caggcaccaa agtaacttgt tcgggcttac attctagcac caaatcgata
     9241 aaattatgtt gcggatttcc ttcaatatta aattctgtag tcacaatgga ttttaaatct
     9301 cgtgcatctt gatagcgaat atgtcgctca tctggtcttg ggtgaatcgt aattccttgt
     9361 gcgccaaatt tttgaatatc agtggcaact tttaataaat caggaacatt cccgccacga
     9421 gcatttctta aagtagcaat tttattgatg tttacgctta attttgtcat tttgtatctt
     9481 taaaaataaa attttattta tgtgattaca aaaatacaaa gttaaggaag tcatcatgtc
     9541 ctaaaatttg attattttgc agtcgctaac agaaaaaaga acatgagaat actttcagaa
     9601 tatattaata atgattttaa gccttttttg atttcggaga atgtatcgga aattcaagat
     9661 ttttttgccg aaaacacttt ttcacatttt ccagttgtgg acaatcaagt atttttaggc
     9721 tgtatttctg ctaatgattc ggaaactttt gaatttggca aaacgcttgc tgattatcgt
     9781 tacgctttcg aaggtttttt tgtaagagaa cctatgattt ggcttgatgt tttagaaata
     9841 tttgccagaa acaatgccaa tattgttcct attttaagcg aatccaatca atatgttggc
     9901 tattacgaaa ttacagatgt tattaaattt ttaaacgaaa cacctttttt aaaagagact
     9961 ggtggaatta ttgtagtaga aaaattaacc gttgattact ctatgagtca aattgctcaa
    10021 attgtagaaa gtaacaatgg taaacttctc ggattatttg tctcagaagc ttctaatgac
    10081 aaagttcaag taactgtaaa aacaactctt gtcggattga atgaaattat tcaaactttt
    10141 agacgttata attatgaaat catctctgaa cacaatgaag atgactatct caataactta
    10201 aaggaacgct ctgaatatct tgacaaatat ttaaatatct aactatgaaa gtcgccattt
    10261 ttggacaata ttatcaaaat gatacccgac caattatcaa ggatattttt gttttcttta
    10321 atagaaatga agtagaatta gtcattgaag aaaaatttct taaaattcta tacgaagaaa
    10381 aaatcataga aaaagaatac aataccttta aatcaggcac tgaattagac caaagttttg
    10441 acatactaat cagcattgga ggcgatggaa caattctccg agcagcaaca tatgttggca
    10501 actcaggcat tcctattctt ggcgttaatg ctggaagatt aggcttttta gccaaggttc
    10561 aaaaagaaga cattcaatca tttcttcaaa ttgtattaga aaaaaaatat accatttccg
    10621 aaagaacact gttaaagctg gaaacgaatc caaaaaatga tgaaatagaa attgattttg
    10681 ccatgaatga aatttcagta agcagaaaag ataccacttc tatgattact attgaaactt
    10741 cgttgaatgg tgaacattta aattcttatt gggcagatgg attgattatt tctacaccaa
    10801 ctggttcaac aggatactca ctaagttgtg gcggtccaat tttaacaccc gatgtcaagg
    10861 gatttgttat tacgccaatt gctcctcaca atttaaatgc tcggccattg gttattcctg
    10921 acaacaccga aataaagtta aaagtatcag gtagagaaga acaatatctt gtctcattag
    10981 attccagaat tctttctata aacaacaatt cagaactttt tattcgcaaa aaccactttt
    11041 ccataaacat ggttgaattt cctaatgaat ccttttttaa aacacttcgc aaaaaattac
    11101 tttggggtga agacaaaaga aattaaaaaa atatccactt taaatatact actttacatt
    11161 ttttttcgtt ttaaaactac catagtgtaa ctttacttag ctgattactt ttattttatt
    11221 tgccataaat acgattttta tgcacaataa gtactcagaa ttattatatt tgcacgctat
    11281 tttaaataga tgaaaaagaa tttagtttta ttttttttaa tgatatccac tcattttttg
    11341 aatgctcaaa tacatgaaat tggtgttttt ttaggtggaa gcaattatat tggagatgtt
    11401 ggaaaaacga attacatcaa tccaaacaaa attgcattcg gaattttata caaatggaac
    11461 aaaagtccaa gacattccta taggttttcc tatacacaat caagcattat tgcaaatgat
    11521 gccaattcaa aaatactagg aagagaatta agaggatacc aatttaaaaa cagcattaaa
    11581 gaattttctg ctggattaga gtttaatttt tttgatttta atctgcatga agctttaaaa
    11641 atgaaaacaa caccttatgt atattcggga gtttcctatt ttatatatga tgaattgtat
    11701 gtcctaaatg gtgtaacaaa aaatgattat caaagaggcg aatttgctat tccaatgatt
    11761 gttggcgtta aaagcaaatt gactgaaaca ttaattatag gatttgaagt tggtgcgaga
    11821 tatacattta cagataatct agacggaagt ttgccaaaga acggaaattt acaatcctta
    11881 aaatttggaa atataaatag taatgattgg tatgttttca ctgggtttac gttaacatac
    11941 acttttggaa acaaaccttg ttattgcgca gaataaaatg agtatagaaa atattgatac
    12001 taaaaaacta ccaaaacatt tggccattat tatggacgga aatggtcgtt gggcaaaaca
    12061 aaaaggatta cttagagctt tgggtcatga aaacggaacc aaatctgtaa aagcaacggt
    12121 tgaatcttgt gccaaattag gaatcgaaaa tctaacttta tatgcttttt ctactgaaaa
    12181 ctggaacaga cccaagcttg aagtcgatac tttaatgaaa ttactaatag cttccttaaa
    12241 aaaagaatta gaaacgcttc aaaaaaataa catccaatta aactctattg gaaacattga
    12301 cttattgcct tcaaaagcaa aaaaagaact tgaagcagta atagaaaaaa caaaaaacaa
    12361 tacccgaatg acattgactt tagcactcag ttatggcgct agagaagaat tgttaaatgc
    12421 tgttaaaaaa ataagtgata aagttaaaaa taatataatt tctcttgata gtattgatga
    12481 atcaattatt aatcagcatc tttacacaca caatttaccc gatgtagatt tggtaattag
    12541 aaccagcggc gaacacagaa taagtaactt tttgctatgg caaattgcct atgcagaatt
    12601 ttattttact gatgttcttt ggcctgattt taaggaagat gatttatatc aagcgattat
    12661 aagttatcaa aaaagagaac gaagatttgg aaaaacaagt gaacaaatta aatagaattt
    12721 tagtgcaaat aaatacttta aaaatagttc taacagtact tcttgtcgga actgtttttc
    12781 aattacaagc ccaagataga ataccatttg atcaaggacg aaaatacatt ttagccgacg
    12841 ttgaagtagt tggtaaaatt agttataacc aacaaactgt tgtaaccttt tctgggttag
    12901 aaaaaggaca agaaattacc gttccaggag aacaaattag caatgctatt aagaaacttg
    12961 gtaaacttgg tctatttagt gatattgatt tttacataaa taagactcaa aacgatagta
    13021 tttggttaga acttgctatt agtgagcttc caaaattaag tgaggtaaag attcaaggcg
    13081 taaaaaaatc aaaagttgaa agtttaatta aagacaactc cttaacaaaa ggtaaaattg
    13141 ttaatgaaaa tttaattaca actaccaaaa attatattga aaacaaatac aaaaaagacg
    13201 gatattacaa caccaaagtt ttcataaaaa caatcgctga tactactgaa ggaaatcaag
    13261 taaaaatgtt ggttaacatt gacaaaggtg ataaactaaa aatagcaaga attgactttg
    13321 atggtaatga aaaattctca gacaaaaaac tgagaggcgc catgaaaaat acaaaaaagc
    13381 aaaactttat tagaattttc aaagcttcaa aatttgtaaa agacaaatac aaagaagact
    13441 tggtatccat cgttgacaaa tacaaagaaa aaggatatcg cgatgctcga atcatttctg
    13501 attcagttta tttaaacgaa aagaaaaaca aaatcaacat taatattaaa cttgaagaag
    13561 gcagaaaata ttattttgga gatattaaat tccttggtaa ttctgtttat tctgatcaat
    13621 tattaagtag agttcttggc attaaaaaag gcgaaactta caatggtgtt ttactacaaa
    13681 aaagaattgc tgacaaaaca aaaccagatg cagaagattt gaccaattta tatcaaaaca
    13741 atggctattt gttttcaaac atcaatgcgg ttgaagtaaa aactgaaaac gacactattg
    13801 atttcgaaat tagagtagta gaaggcccta tcgcctattt taataacatt acagtggttg
    13861 ggaacgataa aaccaatgac catgtaattt atcgtgaatt aagaacacgt cctggagaaa
    13921 aatatagtaa agaagattta gtaagaacca ttagagaaat tggtcaatta ggtttctttg
    13981 atccagaatc tatagaccca aaattcaaaa atgttgaccc agcatcggga acagtggata
    14041 ttgaatacaa tttagttgaa aaagggtcaa gtcaaattga acttcaaggt ggctacggcg
    14101 gcggcggatt tattggaacc ttaggactat ctttcaacaa tttctctgtt agaaatatgt
    14161 ttaaaaaaga tgcctacaaa ccattaccaa tgggtgacgg tcaaaaagta tctttaagag
    14221 cacaagcaag ttcctttttc caaacttata gtttatcttt ctcagaacca tggtttggcg
    14281 gaaaaaaacc aatacagttt tcatcttctt tgtcacacag taaacagttt ttatattcag
    14341 gaaattcggc aagcgttgat aaaactagaa gcttcaacat tacatcgcta tctgtaggaa
    14401 tctcaaaacg tttaactgtt ccagatgata atttctattt ttctaatgcg gtaagttttc
    14461 aatattatga tttgaataat tataatactg gtttatttac ctttggagat ggatcctcta
    14521 gaaatttagc tttcacactt ggacttacta gaaacaacaa aggaataaat ccaatttatc
    14581 caacatctgg ttcagaattt agtgtctcgg ccaaatttac cttaccttat tccttattta
    14641 acggtattga ctatggaaat ttaggaaatc ttatagaaaa taaactaaca tatgatccat
    14701 caaagggatt aaatgtacaa ccaacttctc aagggcaagt tccttcaatt ggcgattatc
    14761 tgggagtggc tcaaacagta aatggacaaa caatttatca agttgttgac acctatcaag
    14821 aagcagcggc cgaccaatca aaagttgatc aattaaaatt caattggtta gaatattata
    14881 aattaaaatt caaagccgat tggtacacca aagtagctgg ctctcaacaa aaagcattgg
    14941 ttttaagagc tttgggtgaa tttggttatt tgggtgcata caacaatgcg agaggcgttg
    15001 ttccttttga aagatttttt ctaggtggag atggtcttgc gaattttgct cttgacggaa
    15061 gagaagtagt acaattaaga ggttatccaa atcaatcatt atcctcagtt gatggtggaa
    15121 ccatttataa taaattcact ctagaactta gatacccact aactttgaaa gcagctgcat
    15181 ctatttatgc tttgactttt cttgaagctg gttcgtcatt tgatagtttt caagaataca
    15241 acccttttgt gcttcaacgc tcggcaggat tcggtttaag agtattcatg cctgcgtttg
    15301 gactacttgg tatcgacttt ggtcatgggt ttgatcctct accaggtgga acccaaaaga
    15361 acggttggga gacacatttt ataataggtc aacaatttta attatatttg aatttaatta
    15421 taaatttatt ctttatgaaa aaatatattg caatccttat tattttgctt acaagcaccc
    15481 aattgaatgc ccaaagcaga ggtgtaaaaa ttggttatat tgatatggaa tacatccttc
    15541 aaaatgtaac cgactattcg gaagcaaaaa accaattgga acaaaaagcc caaaaatgga
    15601 agcaagatat tgaagctaaa aaagtggaga ttgctaaact taaggacgaa ttaaaaacag
    15661 aaaaaccttt attaactaaa gaattaatag atgaaagaga agaagaaatt gcttttcaag
    15721 aacaagaatt attagacttt caacaaaaaa gatttggtcc aagcggtgat ttaattactc
    15781 aaaaagcagt tttggtaaaa cctatccaag atcaagtttt taatgctgtt caagatattg
    15841 ctgaacaaca aaaatacgac ttcatttttg acaaatcatc agacttaacg atgttgtttg
    15901 ctgcaaaacg atatgatatt agcgatagag ttttaagggt tttaaaccgg tcagaaaaaa
    15961 gacaacaaat gtctgcaaaa cagttaaaag agcaggagaa aaaagaagct ttagaagaca
    16021 tggaagatga aaatccagcc atagccgaaa gaagaaaaaa attggaagaa cgaaaagcgg
    16081 ctcgtgacaa actattagaa gaccgaaaag ctgctgctga ggctaaaaaa gccgaacaac
    16141 aagcaaagag agacgcagct gcagcagaaa gagaagctaa aaaaaatggc acggttaatg
    16201 cagatgaaaa accatcaagt gaaaacacaa ctgaaaaaga ctctacggca acaacaacaa
    16261 aagaagataa agccgctgaa agagctagaa tattagccga aagaaaaaaa gaattagacg
    16321 ataaaagaaa aaaaatacta gaagaaagag aagctgcaaa aaaagctaga gaagaaaaaa
    16381 ataacgaacc taaaacaaat taaaaataaa ataaattaat taatactttt aaaacaatga
    16441 aaagattgaa atcattacta atagctggag ttttgttttt tggaacaatg caaacgatta
    16501 atgcacaagc taaaactgca cacgtagatg taaatgaaat tatttctaaa atgcctgcga
    16561 tgttagactc tcaaaaacag ctggaaaaat tgagtgctac ttatgatgcc gagtacaaaa
    16621 caatggcaga agaatatcaa gctaaaatta agaaatacga ccaagaagct tcaacagtaa
    16681 cggatgcagt taatcaaaca cgtcaacaag aagttcaaga tttagtaaaa agaattactg
    16741 attatagaga caatgctcaa aaagaattgc aaaaaaaaga atctgattta gtaaaaccat
    16801 taatggataa aatcagagct tcaattgcaa aagttggtaa agcaaaaggt taccaatacg
    16861 ttctaaatgc tgctgattta ttattaaccg atggtccaga tttaacagct gatgttaaaa
    16921 aagacctagg tttctaaaat ttaactatca ttattataaa gcagctcaat tttttgagct
    16981 gttttttttt acttttgttt ttatgataaa taataatcct atagggcttt ttgattcagg
    17041 cattggtggt acatccattt ggaaagaaat tcatgattta cttccaaatg aaaacacaat
    17101 ctaccttgct gatagtaaaa acgcacctta tggacaaaag tctaaagaag aaattgttca
    17161 attaagcata aaaaacacag agtttttact gaatcaaaat tgcaaaataa ttgttgtagc
    17221 atgtaatact gcaactacca acgccatcaa agaattgaga gctaaatacg atgtgccttt
    17281 tattggaatt gaacctgcca taaaaccagc agctttaaat tcatcaacaa acactattgg
    17341 aattcttgcc accaaaggaa cattgaatag tgaattattt caccaaactt tagaaaagtt
    17401 tcaagaaaca aaaatcattg agcaaatagg acatggttta gtaaccttaa tcgaaaacgg
    17461 agaactcaat tcaattgaaa cgaaaaacct tcttaaaaca tatttaaaac ccatgattga
    17521 agccaatatt gactatttgg tgttaggctg cagtcattac ccctacctta ttcctcaaat
    17581 aaaaaaaata attccaacta aaataaaaat tattgattcg ggagaagctg ttgcacgaca
    17641 aaccaagaat gtattagttg aaaaaagcat tctaaacctt gaaaacaaat tgggaaaagc
    17701 tgttttttat accaatgcca atccaaaagt gcttgaaaac atacttaaaa ataaagaaga
    17761 agtcattcaa aaagattttt agaagtttgt gttttgaatt gttattattt caaggtttaa
    17821 gaaaccttga aaattaaaac tatttctcat tttcattttt aaaccaaccc gaatacatta
    17881 cataattatt agaaatacgt tcaatttctc caccaaaatc tgattgatta atttccttga
    17941 cttttcgagc aggaacacca gcatagatac ttcccgattc aataactgtg ttttgagtaa
    18001 ctacagcacc tgcagctaca attgaattgc tttccacaac acaattatcc atcacaatgg
    18061 cacccattcc tatcaaaaca ttatcatgaa ttttacaacc atgaacaata gcattgtgtc
    18121 caatagaaac attatttcca atttccgtcg gatgtttttg atacgtgcaa tgaataattg
    18181 caccatcttg aatatttact ttattcccaa tttttatgaa atgaacatca cctcgaatta
    18241 cagcattaaa ccaaacgcta catgaatgtc caaaagtaac atcgccaact attgtagcgt
    18301 tttctgctac atagcaatcc tcaggaattt gaggtttctt tccatttaca gttttaagta
    18361 tcatataaaa aaagtgtccc gaatgaatcg ggacatttat taaaatcatt aattaattgc
    18421 tggacaatta cagtggtatt tttcaggctt acagaacaaa ttaattccta aagtcaactg
    18481 atgaaaacca gccgaatcaa actttacatt tccaagcaat tgagaataag tgtacgataa
    18541 catataattt ttaaaattaa ccccaagaat cggtgttatg tattgcaact tttggtctcc
    18601 aatactttgt ccttctacat attgagcacc atcaaggctt cttctgtaag aaataccacc
    18661 ccaaagcatt ccgaaatcta aatttttata cgccttaaca ttcaaatcaa ttgttttttc
    18721 ttgagtttga gttaccaatt gaaacataaa agaaggttcc cataaaattt tatctgcatc
    18781 accaaaagtg taacccgaat ttaatataaa tcttctcaaa tttacaggct ctctatc
//
LOCUS       DAOOBH010000034        18695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_3924_length_18695_cov_5.615558, whole genome shotgun sequence.
ACCESSION   DAOOBH010000034 DAOOBH010000000
VERSION     DAOOBH010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 18695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18695
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3924_length_18695_cov_5.615558"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PK218_05375"
     CDS             <1..216
                     /locus_tag="PK218_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-like protein"
                     /protein_id="HPW97972.1"
                     /translation="NILFYTGNFDGNPMMTHKTLNEKAPMDFTHFNRWNTLFDETVDA
                     LYKGEKAEEIKNRAMNISKAMMDKALN"
     gene            complement(161..1732)
                     /gene="nadB"
                     /locus_tag="PK218_05380"
     CDS             complement(161..1732)
                     /gene="nadB"
                     /locus_tag="PK218_05380"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPW97973.1"
                     /translation="MTQTNYLIFGSGVAGLTFAIKIATHFPQKNIVIVTKSNEAESNT
                     KYAQGGIAVVLNQIEDNFQKHIDDTLKCGDGLCVKAVVEKVVKEGPKCVHELIDWGTR
                     FDKDETGGFDLGKEGGHSSHRVIHHKDQTGFEIERAILKQAHLQKNITLLDYHFGLDL
                     IVKNNSCFGATVLNEKTNEISTYLADYVLLATGGIGQIYGHTTNPSIATGDGIAMAIR
                     AKATVKDMEFIQFHPTALYNPELKSTFLISEAVRGFGAILRDKNGRRFMFDYDVRGEL
                     ASRDIVSQSIENELKKSGDDCVFLDCTHLDTMAFKKHFPMIYERCKDLTILIERDWIP
                     VIPAQHYVCGGIKVDGNGETTIQNLFACGECSYTGLHGANRLASNSLLEALVYADSIF
                     NFLSTKTASMPKFIPLNCNYKFNKKGSIKLNHVEEIVYEIQEMMRKNVGIIRNNEDLE
                     TTFKILSNLQHVLVSIEETHQATREIYELKNMVEVAKIIVNQSLKRTTNRGSFFKANL
                     GLYPSSLSKYSSLYF"
     gene            complement(1735..2661)
                     /gene="nadA"
                     /locus_tag="PK218_05385"
     CDS             complement(1735..2661)
                     /gene="nadA"
                     /locus_tag="PK218_05385"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002665330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPW97974.1"
                     /translation="MNTLKEKVLQLKKEKNAVILAHYYQEVAIQEIADYVGDSLGLSQ
                     VAAKAEADIILFAGVHFMAETAKILNPTKKVILPDLNAGCSLADSCPPNLFKKFIAAH
                     PDHVVITYVNCSAEIKALSHLVCTSSNAVKIVESVPSSIPIIFAPDKNLGKYIMEQTG
                     RNMVLWDGSCIVHEAFSIDKLLELHKRNPDAEIIAHPESETHILKVAHYIGSTAGMIA
                     YVKESKKHKFIVATEAGILYKMQQQAPNKVLIPAPSHEDNTCACSECAFMKVNTLQKV
                     YDCLLNETPEIQIPKSIQKKALLPIDRMLQLS"
     gene            2801..3526
                     /gene="ric"
                     /locus_tag="PK218_05390"
     CDS             2801..3526
                     /gene="ric"
                     /locus_tag="PK218_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HPW97975.1"
                     /translation="MNVLEQITIGEYVAKDFRTAAVFSKYGIDFCCKGNRTIEEASEK
                     KNLDFVQIEKEITQLLETKSDGAIDFRSWPSDLLIDYIEKTHHRYVEEKSSVLLPFLD
                     KLCKVHGERHPELFEINELFKGCARELAQHMKKEELILFPFIKKMENAIRTGKAIEAP
                     HFGTVENPIEMMKDEHVTEGERFVKIATLTNNYTPPADACNTYKVTFSMLQEFEQDLH
                     KHIHLENNILFPKAIEMQSKFII"
     gene            3547..5661
                     /locus_tag="PK218_05395"
     CDS             3547..5661
                     /locus_tag="PK218_05395"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPW97976.1"
                     /translation="MELYVIKRNGAYKPFEGYKIKDAIEKSFKSVAVPFDESVFSVVL
                     NLLEQKEVWAVEEIQDNIEKTLFEKQYFSVMRSFMLFRHTRKLQREHVAGLNDDTTYV
                     DSTQTIEEYISQTDWRINANANTSYSNAGLVNNVAGKIIANYWLDKIYTKEEGYAHRN
                     GDLHIHDLDCLSGYCAGWSLRVLLNEGFNGVRGRVESKPPSHFREALGQMANFLGILQ
                     SEWAGAQAFSSFDTYLAPYVFKDNLEFDDVLKAVRSFVYNLNVPARWGQSPFTNITLD
                     WVVPDDLKVQVPTKNDIHLFRGIDNQSMLARAKSRGVSNLEEMRYEHFQTEMNLINKA
                     YYTVMTEGDTNGQPFTFPIPTVNITEDFDWDGENTELLFENTAKTGSSYFQNFIGSQY
                     ILDENGNKIENENAYKPNAVRSMCCRLQLDLRELLKRGNGLFGSAEMTGSIGVVTINM
                     ARLGYLYADDKEALYTQLDALLEIAKATLEKKRVFIQEMYDRGLYPYTKRYLSHFRNH
                     FSTIGVNGMNEMIRNYTQDKEDLTTSFGHEFSMEILEYIRNKMKEYQEATGNLYNLEA
                     TPAEGTTYRFAKEDLKRYPAILQAGMEENVYYTNSSQIPVDFTQDPFEALLLQDELQC
                     KYTGGTVLHLYMNERISSPEACKNFVKTVLTNFRLPYITVTPVFSVCPIHGYLNGEHE
                     YCPKCDENLIENHNNTTYENKN"
     gene            5645..5815
                     /gene="nrdD"
                     /locus_tag="PK218_05400"
     CDS             5645..5815
                     /gene="nrdD"
                     /locus_tag="PK218_05400"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPW97977.1"
                     /translation="MKTKTNEANLLTDQQHLRTKCLVYTRVMGYHRPVESFNIGKKGE
                     HKQRLHFKENVC"
     gene            5809..6486
                     /locus_tag="PK218_05405"
     CDS             5809..6486
                     /locus_tag="PK218_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPW97978.1"
                     /translation="MLTKAISSITPFTLLDYPHKAACILWYAGCNMRCLYCYNPEIVL
                     GKGTIAFDAILSFLDKRKGLLDAVVFSGGECTLHKSIYNHIMQVKTLGFLVKIDTNGS
                     KPELLQRLIDENALDYVALDFKALPSKFQYITQSKLYAAFKQSLVILLANTIPFEVRT
                     TYHSELLNEEDIKKMIVFLETHHYVGNYYIQHFRNNTKTLGNLSYSKKNLLEKNLSTA
                     TINVVFR"
     gene            6755..7381
                     /locus_tag="PK218_05410"
     CDS             6755..7381
                     /locus_tag="PK218_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97979.1"
                     /translation="MRKRIHILIFSLITTFAFGQTEKESFWNPYIESDRIEIGLKHYD
                     SINFEKAYRIWSDYQVVELMKINDTTYRGTLTNFVTQNKRKNKKKIIYQKIKIPNETV
                     ERLINTLYIENIETLKDCSEIENYPKGFDGKTYVFEIGKEKTRRVYSYWEPENERYQN
                     PEMSEIKNVRKILSSINTEFNLWKYFKDFRDRLPKGSYSYGMINMIKI"
     gene            7645..8154
                     /locus_tag="PK218_05415"
     CDS             7645..8154
                     /locus_tag="PK218_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97980.1"
                     /translation="MTYETYWSLLDTSAKSIRSYDVFLYIIVISFIAWLVTKKFKKSN
                     GDIEKLILLWFAGIVFAISLSGFIYLKLYDNDESYENTRKFLESSQVERVEGIITNFN
                     SGPIASRRGVTQESFLVDSIYFYYSDELLGRFNQFGKTNNGVLKNGLQVRITYGGNRH
                     QIYKIEIAK"
     gene            8505..8948
                     /locus_tag="PK218_05420"
     CDS             8505..8948
                     /locus_tag="PK218_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97981.1"
                     /translation="MKNLITLLTVFIFILISHDCKAQVYDEPPEVAMPSNENKQLIDK
                     IIEVTHYESYFNKYCIKYISKVAKQENWNKERLEKAKKMVSLSSFKFNIYNWSSNYSN
                     AQLKEYLELYKKDKSSKKKNFFIENPNIAKALESKTKQFITESGK"
     gene            complement(9093..10328)
                     /locus_tag="PK218_05425"
     CDS             complement(9093..10328)
                     /locus_tag="PK218_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW97982.1"
                     /translation="MKTWKKTTQSDRLERITKALSENVNFSNDVTLGYPASKLDGKVF
                     YDDASFLKDAPVLMTYVANPNHIGCHTVGISEKAFKGTQEIEREVLEILAVDVFKATT
                     NDYDGYIATGGTEANIEALWIYRNYFINTHHAKPSEIMIITSEDTHYSIAKGSNLLMV
                     DWMKVDVHFENRKINETKFETALENAMANGKKYFIVVANMGTTMFGSVDNPDAYTTIL
                     EKHNLHFKLHVDAAYGGFIYPFLTDKNQLDFSNPHVSSITIDAHKMLQAPYGTGIFLC
                     RKNLIENVLTKEAAYVEGMDLTLCGSRSGANAVAVWMILKTYGKNGWFEKVKILEMRT
                     DWLCKQLDALHITYFREPFMNIVTIKAAFISPEIAHHYDLVPDSHQDNNHWYKIVLME
                     HVEVGSLQELINTLKTTKT"
     gene            complement(10330..12126)
                     /locus_tag="PK218_05430"
     CDS             complement(10330..12126)
                     /locus_tag="PK218_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97983.1"
                     /translation="MSNKKNSHSFHIPVMGLAFTIDSPIRVAHYGISSTVSIIDDDLA
                     EKMRAFYSEKFSIPYQEITQKIQDYRAKRITNYLNTMDSIVNEKFENFKKELAESKTT
                     LENYIALLPNKSDLKKGLQEFIDSGISMKDTLINYIEKNLSLGEIDVNIMTKVDKDNY
                     EGDVQLPTEFNDAHASLRGFANSNLKSSVVLSAGMNPRLYSYFESFPDFFPDENGFLK
                     KKIILKVSDYRSALIQGNFLAKKGLWVSEYRIESGLNCGGHAFATEGFLMGPILQEFK
                     EKRQELLQSTHDLLIKALTIKELPLPENSMPIKISAQGGVGTAEEHNFLLEEYQLDSI
                     GWGSPFLLVPEATSVDAETRKLLANAKEDDFYLSNISPLGVPFNSLKGVTNDFWKQKR
                     INENKAGSSCPKKFLALNKSYNKEGICTASKKYQDAELEKLNQQKSEISTSDFEIKQQ
                     KITEKSCLCVGLANASYLEHEMPIKGQQQGVVICPGPNLAYFDAEYSLKEMVQHIYGN
                     ANVMLQENRPNLFVKELKMYVDYLKNEICTFTEEKSANQLKKWNAYKNNINLGIEYYH
                     NLLKNTAFFKNNQDTMHQQLDFYKAEINAINI"
     gene            complement(12136..13470)
                     /locus_tag="PK218_05435"
     CDS             complement(12136..13470)
                     /locus_tag="PK218_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HPW97984.1"
                     /translation="MENNKYPFYFIITALISLFLGMSFGLLGGLQYIFPDFIKEKLPF
                     NAIRPLHTLFVVSWILLAAIGGIYYYLKSNLNLKFYSNTLVKAHFWLFILTGIGIAFS
                     YVSKNFAGKEYLEFPSYFYFPIVLGWILFGINYFKSMFSSFKTWPVYYYMWSTGIVLM
                     IFHFTEAHLWLLPYFRDNFIQNVAIQWKAGGSYVGSWNMLVYGTALFVMEKISDESRY
                     AISKKAFFFYFLGLTNLMFGWAHHIYMVPTAPWIRYFAYIISMTEWIILFSIIYDWRK
                     NLLQEKKVHFSIAYKFMILADLWVFLNIIVALLISIPAINLFTHGTHITVAHSMGTTI
                     GINTLILFSSITYIFNTAFTFSERTIQKLKKGIQIFHISFFLFWTTLLIMGMKKSFWL
                     FGNSSETFSKFQESQHGWHIAFVLFGLGIITGLGIIMLQLIKTFQQNKFKNI"
     gene            complement(13460..13897)
                     /locus_tag="PK218_05440"
     CDS             complement(13460..13897)
                     /locus_tag="PK218_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPW97985.1"
                     /translation="MSQSSKYLILILVFICVFSIYNFIIYTSKSEYKTIHLSQKALKG
                     ENIWLQNNCNSCHQIYGLGGYLGPDITNVYSREGKGEKYIKTIVNSGIKSMPKFNFSE
                     EEKDQLVQFFKEIDQTGHYPDANATIGKDGWVTIKNKLLENGK"
     gene            complement(13907..14344)
                     /locus_tag="PK218_05445"
     CDS             complement(13907..14344)
                     /locus_tag="PK218_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPW97986.1"
                     /translation="MEKHTYTVNLIWENDRKGIMSSPELATKIEVATPPEFDKGMPNI
                     WSPEHLFTASVVSCFMTTFLAIAEYSKLEYIKFSCNAEGVLEKIDGKYLMTKIILKPE
                     LIIAELDKMERAQRILEKSEAACLISNSIKSEIELIAVINIEN"
     gene            complement(14505..15062)
                     /locus_tag="PK218_05450"
     CDS             complement(14505..15062)
                     /locus_tag="PK218_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPW97987.1"
                     /translation="MKKSILFLAIASLILTTSCKENTEATKETNTTESNVDLSVGQEG
                     VQDEVSNPNIVQVASGSKDHTTLVAAVKAAGLVTSLSNAGPFTVFAPTNAAFDKLPAG
                     TVEGLLKPEKKADLENILGYHTYVGNLKAEYMQDGQEFDMVYGGKVKITKQGDKTFVN
                     GSEIVASIPTSNGIIHVIGDVLLPK"
     gene            complement(15130..16359)
                     /locus_tag="PK218_05455"
     CDS             complement(15130..16359)
                     /locus_tag="PK218_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97988.1"
                     /translation="MPFDTKFWIKFSLINLSIVALLGLVMRYKIGFEFSLFNQKNIQH
                     SHSHFAFSGWITHTLMVVMISYLTTKVLINSKKYILLLILNLVCSYGMLFSFFISGYT
                     AISIIFSTASIFVSYVFAVYFYNDLKRIDSDDLSKNWFKAAVFFSVISSFGTFFLAYM
                     MMTKNIHQEEYLASIYYYLHFQYNGWFFFACMGLLFSFLNLTSALNPFLKQASKLFFV
                     SCIPAYFLSTLWLNLPNWIYFLTIIAAFIQVYAWFRVLVIILKTKLEFIKNQAIFLRY
                     ILGFIGLAVSIKFLLQLGSTIPALSQLAFGFRPIVIAYLHLVLLAIISLFLLFYIYAK
                     HTFYMHKKLKFGIILFTVGIFLNELALAIQGIASFSYTLIPFINEILFGIAILLFLGI
                     IITSYFSIKKVKINANL"
     gene            complement(16361..16951)
                     /locus_tag="PK218_05460"
     CDS             complement(16361..16951)
                     /locus_tag="PK218_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPW97989.1"
                     /translation="MQIDLDILFTWGAIAKVYKKNETVFFEDEMANFYYQIIEGSVKM
                     FNSNDEGKEFTQGYFSNGQSFGEPPLFIDEAYPATALACQESKIIKLSKDKFLKIMDE
                     YPQIQKKFLKTLASRIHSKANTAKDIINQKPEFRVLSFLNSIKKTSETNQKELVPFTR
                     QEIANFTGLRVETVIRVFSKLKTCKKVDIIKHKIYY"
     gene            17081..17455
                     /locus_tag="PK218_05465"
     CDS             17081..17455
                     /locus_tag="PK218_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97990.1"
                     /translation="MKSLIMMMALTMLLKPVFPVVEYIVNYDYIAKVLCENKTKPELK
                     CNGKCHLMKEMAKQAENEKPIQNNKKQATSEFEVLFFQEIASLQFTQSNGDDKSITLD
                     CYSNHYFHLNSDAVFRPPIFSC"
     gene            17565..18407
                     /locus_tag="PK218_05470"
     CDS             17565..18407
                     /locus_tag="PK218_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW97991.1"
                     /translation="MKNQLKKIALVLITLAFASCSNDDNQTNTNELDGLTKFKEVVEG
                     NQTIEFYSPKGALSQGYNDIKFRIKNNETNQYITDAQVEWMPKMHMTMMTHSCPKSEV
                     VKAATGNLYEGYIVFQMAQNETEYWDLTLDYSIDGEEHSLIIPIDVPATAKRVVSSFM
                     GSDNVRYVIAYIDPTSPKVGMNDLKVGIWKMQDMMTFPMINDYTVKIDPRMPSMGNHS
                     SPNNVNAQQTTVDNFYIGKLSLTMTGYWKINLQLQNDLGTIVKGEEITDSVTESSLFF
                     EIEF"
     gene            18471..>18695
                     /locus_tag="PK218_05475"
     CDS             18471..>18695
                     /locus_tag="PK218_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW97992.1"
                     /translation="MKEILSFLLVFCGFNSVISQETHQDTISPKQLKEIIVIGKKTQV
                     FEKQTKSLSTVDEYLQKSSRVGMIKRGAYAW"
BASE COUNT         6303 a         3032 c         3287 g         6073 t
ORIGIN      
        1 aacattcttt tttatactgg aaattttgat ggtaatccga tgatgacaca caaaacgttg
       61 aacgaaaaag cacctatgga ttttacgcat tttaatcgtt ggaatacttt gtttgatgaa
      121 acggttgatg ctttgtataa aggcgaaaaa gcagaagaaa ttaaaaatag agcgatgaat
      181 atttcgaaag cgatgatgga taaagcccta aattagcttt aaaaaaacta ccgcgattag
      241 ttgttctttt taaagattga tttacaataa tttttgcaac ttctaccata ttcttcaatt
      301 cataaatctc ccttgtagct tgatgggttt cttctattga cactaaaacg tgctgcaagt
      361 tggatagaat tttaaaagtc gtttctaaat cttcattatt tctaataata cccacattct
      421 ttcgcatcat ttcttgaatt tcataaacta tttcttctac atgatttagt ttaatactgc
      481 cttttttgtt aaatttataa ttgcaattta gtgggataaa tttaggcatt gaagcggttt
      541 tagtgcttaa aaagttaaaa atactgtcgg cataaaccaa tgcttctaat aatgagttgg
      601 atgctaatcg atttgctcca tgaagtccag tataagaaca ctcaccacaa gcaaataaat
      661 tttgaatcgt tgtttctcca ttaccatcaa ctttaatacc gccacataca taatgttgtg
      721 caggaattac tggaatccaa tctctttcaa ttaaaatagt taagtctttg cagcgttcat
      781 aaatcatagg aaaatgtttt ttgaatgcca ttgtgtctaa atgtgtgcaa tctaaaaaga
      841 cacaatcatc accagatttt ttcaattcgt tttcgatgct ttgggataca atatctctcg
      901 atgccaattc gcctctaaca tcataatcaa acataaatcg tcttccgttt ttatctctca
      961 aaatagctcc aaaacctcta acggcctccg aaattaaaaa tgttgatttc aattctggat
     1021 tgtacaaagc tgttggatga aactgaataa actccatgtc ttttactgtt gcttttgctc
     1081 ttatagccat ggcaattcca tcgcctgttg caatagaagg gtttgttgtg tgtccatata
     1141 tttgaccaat tccacctgta gctaaaagaa cataatctgc aagatacgtg ctgatttcat
     1201 tggtcttttc gtttaaaacc gtagcaccaa aacaactatt gtttttaaca attaaatcca
     1261 acccaaagtg ataatcaaga agggtaatgt ttttttgaag gtgtgcttgt tttaaaatgg
     1321 ctctttcaat ttcaaaccca gtttggtctt tgtgatgaat aactcgatga ctggagtgcc
     1381 cgccttcttt acccaaatca aaacctccag tttcgtcttt gtcaaatcgt gttccccaat
     1441 ctattagttc gtgtacgcat tttggtcctt ctttaacaac cttttctact accgctttca
     1501 cacacaatcc gtcgccacat tttaaagtgt catcaatatg tttttgaaaa ttatcttcaa
     1561 tttggtttag cacaaccgca attccacctt gagcatattt tgtattcgat tcggcttcgt
     1621 tcgatttggt tacgatgaca atgttttttt gcggaaagtg tgtagctatt tttatggcaa
     1681 aggttaatcc agcaactccc gaaccaaaaa tcaaataatt tgtttgtgtc atttttagga
     1741 taattgaagc attctatcaa tgggaagcaa cgctttcttt tgaattgatt ttggaatctg
     1801 tatttcggga gtttcgttta aaaggcaatc ataaaccttc tgtaacgtgt ttactttcat
     1861 aaacgcacat tcgctacaag cacaggtgtt gtcttcgtgt gatggtgctg gaattaatac
     1921 tttattaggt gcttgttgtt gcatcttgta taagattcca gcctcggtgg caacgataaa
     1981 tttgtgcttt tttgattcct ttacataagc aatcattcct gcagtggaac ctatataatg
     2041 ggctactttt aaaatatggg tttccgattc aggatgtgcg atgatctcgg catcgggatt
     2101 tcttttatga agttctaata gtttatcaat tgaaaatgct tcgtgaacaa tgcaagaacc
     2161 atcccaaagc accatgtttc tgccagtttg ttccataatg tatttgccta aatttttatc
     2221 gggtgcaaaa attattggaa tacttgaagg aacagattct actattttta ccgcgttgga
     2281 agaagtgcat actaaatgac ttaaagcttt aatttcagcg gaacaattaa cataagtaat
     2341 aaccacatgg tcgggatgtg cggcaataaa ttttttaaat aaatttggcg gacacgaatc
     2401 tgctagtgaa cacccagcat ttaggtcggg taaaataact tttttagttg gatttaaaat
     2461 ttttgcagtt tctgccataa aatgaacacc tgcaaataaa ataatatccg cttcagcttt
     2521 ggcagctact tgagacaaac ctaaactatc acctacataa tcggctattt cttgaatggc
     2581 aacttcttga taatagtgag caagaatgac tgcgtttttt tcttttttta attgaagaac
     2641 tttttctttt aatgtattca tttaaaaata tgcgtttgca ataataaaat attaaagtac
     2701 aaagtaagac acaacaaata tttttaacta tgatttaggt cataatctaa aggatttaat
     2761 agtagtaatt tcgtagaatt aaattttaaa ttatatcatt atgaatgttt tagaacaaat
     2821 aacaataggc gaatatgttg ccaaagattt tagaactgca gccgttttct caaaatatgg
     2881 aatcgatttt tgttgcaaag gaaatagaac catcgaagaa gctagcgaaa agaaaaactt
     2941 agattttgta cagattgaaa aagaaattac ccaactttta gaaacaaaat cagatggtgc
     3001 tattgatttt agatcatggc caagtgattt attgattgac tatatcgaaa aaacacatca
     3061 tcgatatgta gaagaaaaat caagtgtttt gcttcctttt ttagataaat tgtgcaaagt
     3121 gcatggcgaa agacatcctg aattgtttga aattaatgag ttgtttaaag gttgtgcaag
     3181 agaattggca caacacatga aaaaagaaga gttgatttta tttcctttta ttaaaaagat
     3241 ggaaaatgct attagaaccg gaaaagccat tgaagcacct cattttggaa cagtagagaa
     3301 ccctattgaa atgatgaaag acgaacacgt aacagaaggt gaacgttttg tgaaaattgc
     3361 aacattaact aacaattata cgccaccagc cgatgcatgt aacacctata aagtaacatt
     3421 ttccatgcta caagagtttg aacaagattt gcacaaacat attcatctag aaaacaacat
     3481 tttgtttcca aaagcaattg aaatgcaaag caaatttatt atctaaaaca ctgtaaatta
     3541 attagtatgg agctatatgt aattaaacga aatggagctt ataaaccttt tgaaggatat
     3601 aaaattaaag atgctattga gaaaagtttt aaaagcgtag cagttccatt tgatgaaagt
     3661 gttttttcag ttgttctaaa ccttttagag caaaaagaag tttgggctgt cgaagaaatt
     3721 caagataata ttgaaaaaac gcttttcgaa aaacagtatt tttccgtgat gcgttcgttt
     3781 atgttgtttc ggcatacacg aaaattgcaa cgcgaacatg ttgccggttt aaatgatgac
     3841 actacttatg tagatagcac acaaaccatc gaagaataca tctcacaaac cgattggcgg
     3901 attaatgcca atgcaaatac ttcttattcc aatgctggat tggtaaacaa tgtagcagga
     3961 aaaattattg ctaattattg gttggataaa atttacacca aagaagaagg ttatgcacac
     4021 cgaaatggcg atttgcacat tcatgattta gattgtctat cgggttattg tgcgggttgg
     4081 agtttacgtg tcttattgaa tgaaggtttt aatggcgttc gtggacgtgt agaaagcaaa
     4141 ccaccgtcgc attttagaga agccttgggt caaatggcaa actttcttgg tattcttcaa
     4201 agcgaatggg caggagcaca ggcattcagt tcctttgaca cctatttggc accttatgtt
     4261 tttaaagaca atttagagtt cgacgatgtg ttaaaagccg ttagaagttt cgtgtataac
     4321 ctgaatgttc cagcgcgttg gggacaatcg ccgtttacta atatcacctt ggattgggta
     4381 gttccagatg atttgaaagt gcaagtacct actaaaaatg atattcattt gtttagagga
     4441 attgataatc aatcgatgtt agcacgtgct aaaagcagag gcgtttctaa tttagaagaa
     4501 atgcgttatg aacattttca aacagaaatg aatttaatta ataaagcgta ttataccgtg
     4561 atgacggaag gcgataccaa tggccagcca tttacgtttc caattccaac ggtcaatatt
     4621 actgaagatt ttgattggga tggcgaaaac accgaattgc tttttgaaaa tacggcgaaa
     4681 actgggtcgt cgtattttca aaactttatc ggaagtcaat atattttgga tgaaaacggg
     4741 aacaaaattg aaaatgaaaa tgcctataaa cccaatgccg ttcggagtat gtgttgtcgt
     4801 ttgcaactcg atttacgcga attgttaaaa cgcggcaacg gtctttttgg tagtgccgaa
     4861 atgacgggaa gcattggtgt tgttaccatc aacatggcac gactgggtta tttgtatgcc
     4921 gatgataaag aagctttata tacacaacta gatgctttac tggagatagc aaaagccact
     4981 ttagagaaaa aacgagtctt tattcaagaa atgtacgata gaggattata tccttatacc
     5041 aagcggtatt tatcacattt tagaaaccat ttttccacca ttggtgtcaa cggaatgaat
     5101 gaaatgattc ggaattacac acaagacaaa gaagacttga ctactagttt tgggcatgaa
     5161 ttttcgatgg aaattttaga atacatccga aataaaatga aagaatacca agaagcaaca
     5221 ggcaatttat ataacctgga agcgacgcct gccgaaggaa caacctatcg ctttgcaaaa
     5281 gaagatttaa aacgctatcc agcaattctt caagcaggaa tggaagaaaa cgtgtattat
     5341 acaaacagtt ctcaaattcc ggtagatttt acgcaagatc cttttgaagc tttgttgttg
     5401 caagacgaat tgcaatgcaa atataccggt ggaacggtgt tgcatttgta tatgaatgaa
     5461 cgcattagtt cgcccgaagc ctgtaaaaac tttgtgaaaa cagtactaac caattttaga
     5521 ttgccttata tcacagtaac gccagttttt agcgtttgtc caattcacgg ctatttaaat
     5581 ggtgagcatg aatattgccc aaaatgtgat gaaaacttaa tcgaaaacca taataacact
     5641 acttatgaaa acaaaaacta acgaagcaaa tttgctaacc gaccaacaac atttaagaac
     5701 caaatgcttg gtgtacacca gagtaatggg ttatcataga cctgtcgaaa gcttcaatat
     5761 tggcaaaaaa ggcgaacaca aacaacgcct tcattttaaa gaaaacgtgt gctaaccaaa
     5821 gccatttctt caataactcc ttttacactt ttagattatc cacataaagc agcttgcata
     5881 ctttggtatg caggttgcaa tatgcggtgt ttatattgtt ataatcccga gattgtttta
     5941 ggcaaaggca ctattgcttt tgatgctatt ctgtcctttt tagacaaacg aaaagggctg
     6001 cttgatgccg ttgttttcag cggtggcgaa tgtacccttc acaaaagcat ttataaccac
     6061 ataatgcaag ttaaaacact gggttttttg gtaaaaatag atactaatgg cagtaaaccc
     6121 gagctgttgc aacgcttgat agatgaaaat gctttagatt atgtagcctt agatttcaaa
     6181 gccttgccaa gcaaatttca atacattaca caatccaaat tatatgctgc tttcaagcaa
     6241 tcgctggtga ttttgttagc caatactatt ccttttgaag ttcgaacaac ctatcactca
     6301 gaattattaa atgaagaaga tattaaaaaa atgattgttt ttctagaaac acatcattat
     6361 gttgggaatt attatatcca acactttaga aacaatacca aaactttagg caatctttcc
     6421 tattccaaaa aaaatcttct tgaaaaaaat ctttctacgg ctaccatcaa tgtagtgttc
     6481 cgataaaata tccctaaaac tttaaaacaa actcaaacag ttttaaacaa ttattcaaac
     6541 cactatcaaa caaaaaacac cctacctaat atataggaaa aaaaacacca taaataatcc
     6601 cataaaaaca ctataaaaat ccataaccta atcatttata aacacttttt gtatatattt
     6661 gataaacgaa taaagtctga cgtttgtcag ccctatacac ccaaaattgg gtgcataagt
     6721 ctgattttat cagacatatg agttagcaaa cagtttgaga aagagaatac acatattaat
     6781 atttagtctg attacgactt ttgcatttgg acaaaccgaa aaggaatcct tttggaatcc
     6841 ttatatcgaa tctgaccgaa ttgaaattgg attaaaacat tatgacagta taaattttga
     6901 aaaagcgtac cgaatttggt ctgactatca agttgtcgaa ctgatgaaaa ttaatgacac
     6961 aacgtacaga ggaactttaa cgaattttgt aacgcagaat aaaagaaaga acaaaaaaaa
     7021 gattatttat caaaaaataa aaattccaaa cgaaactgtt gaaagattaa tcaatactct
     7081 atatattgaa aatattgaaa cattaaaaga ttgttctgaa attgaaaatt atccaaaagg
     7141 atttgacgga aagacatatg ttttcgaaat cggaaaagaa aaaacaagac gagtttattc
     7201 ctattgggaa cctgaaaacg aaagatatca aaacccagaa atgtctgaaa taaaaaatgt
     7261 tcgaaaaata ttgagttcta ttaacacgga atttaacttg tggaaatatt ttaaagattt
     7321 tagagacagg ttgccgaaag gaagttattc ttatggaatg ataaatatga ttaaaatata
     7381 aaaagcccga ccgcgcggat atgttcaaca gttgatagcg cggatttgca atccgtgccc
     7441 actaacatat ttaaaagcat catctaaccc tagatagttt taacagtaga tttcgctgaa
     7501 ctccagtgat atgttgcatg gttaaaaata cgcaaaactg ttttttcgta caactatttt
     7561 agtatatttg tttactgtga agaggaagtt ttttcacagt ctgacgttta gtggcaataa
     7621 tgttcaagtt ttatcttccg aattatgaca tacgaaactt attggtcact tttagatacg
     7681 tcagcgaaat ctataagatc ttatgatgtt tttttataca taattgtcat ctcttttatt
     7741 gcctggttag taaccaaaaa attcaaaaaa tcaaatggtg acatcgaaaa attaattctg
     7801 ctttggttcg caggaattgt tttcgcaatt tcactttcag gatttatcta tttaaaacta
     7861 tatgataacg acgaaagcta tgagaatact agaaaatttc tagaatcatc acaagtggaa
     7921 agagttgaag gaataattac aaattttaat agcggaccga ttgcatctcg gcgtggtgta
     7981 acgcaagaaa gctttttggt tgactcaata tatttctact attctgacga attactcggt
     8041 cgatttaacc aatttggaaa aactaataat ggagttttaa aaaatggatt gcaggttaga
     8101 ataacttacg gcggaaatcg acatcaaatt tacaaaattg aaattgcaaa ataagtccca
     8161 agttactgcc tataaacaca ggtagctgtt gcacaaccat tgtaaaagca ggcattgaaa
     8221 tacctgccta aaatagcggt cgttcctatg gaactttggc gtgtgctggg tgatgataga
     8281 cggatataag cctccccgct accgcacgaa tcctttcatg gtgtcttcta aactagacag
     8341 cctcatttga ttgcgttgaa ttccagtgat atgttgcatg gttaaaaata cgcaaaactg
     8401 ttttttcgta caactatttt agtatatttg ctggaaggat tgtggaagtt ttttcacagt
     8461 ctgacgttgg cagcaatttt aagacaaccg ctaataaagt gaaaatgaaa aatttaatca
     8521 cattacttac agtatttatt tttattttaa tcagtcatga ttgcaaggca caagtatatg
     8581 atgaaccgcc ggaagtggca atgccatcca acgaaaacaa acaactaatt gacaagataa
     8641 ttgaagttac tcattatgaa tcttatttca ataaatactg cataaaatat atttcaaaag
     8701 ttgctaaaca agaaaattgg aataaagaac ggttagaaaa agctaaaaaa atggtgtcac
     8761 tttcgagttt taaatttaac atttacaatt ggtcatctaa ttattcaaat gcacaactga
     8821 aagaatatct tgaattatac aaaaaagaca aaagtagcaa gaagaaaaat tttttcatag
     8881 aaaatccaaa tattgctaaa gctcttgaat caaaaactaa acaatttatt actgaatcag
     8941 gaaagtaaat aacaaaaact gcctataata gcagtagctg ttgcgcaacc actcccaaaa
     9001 aattaaattt taataataaa tctaaaaaac aacctcgttt agaacaaact aaacgaggtt
     9061 tattttttag caaatcaaca aaacctgcgt tactatgttt tggtggtttt tagcgtattg
     9121 attaattctt gcaaactgcc cacttctaca tgttccatca gtacaatttt ataccaatgg
     9181 ttgttgtcct gatgcgaatc gggaactaag tcatagtgat gcgcgatttc tggtgaaata
     9241 aacgccgctt ttatggtaac aatattcatg aaaggttctc tgaaataggt aatgtgcaga
     9301 gcgtctaatt gtttgcagag ccagtcggtg cgcatttcta gtatttttac tttctcgaac
     9361 caaccgtttt tgccgtaggt ttttagaatc atccaaaccg ctacggcatt agcacccgat
     9421 cggctgccgc acagggttaa atccatgcct tctacatagg cggcttcttt ggtgagtacg
     9481 ttttcaatta aattcttacg gcacaaaaag attccggtgc catagggcgc ttgcagcatt
     9541 ttgtgtgcat cgatggtgat ggaagaaaca tgtggattgc taaagtcgag ttggttttta
     9601 tcggttaaaa aaggatagat aaaaccgcca taagcggcat caacatgaag cttaaagtgt
     9661 aagttgtgtt tctctaaaat agtagtgtag gcatcgggat tatcaacaga gccaaacatg
     9721 gtggtaccca tatttgccac tacgataaag tatttttttc cgttagccat ggcgttttcg
     9781 agagccgttt caaacttggt ttcgtttatt tttcggtttt caaaatgaac atcgactttc
     9841 atccaatcga ccatcaaaag attagagcct ttggcaatgg aatagtgcgt gtcttcggaa
     9901 gtaataatca tgatttcgga aggcttggca tgatgggtat tgataaagta atttcgataa
     9961 atccacaagg cttcgatgtt ggcttcggtg ccgcccgtgg caatatagcc gtcatagtcg
    10021 ttggtggttg ctttaaaaac atcaacggca aggatttcga gtacttcgcg ttcaatttct
    10081 tgcgtgcctt taaaggcttt ttcagaaatt ccaacagtat gacaaccaat gtggtttgga
    10141 ttggcaacgt aggtcatcaa aactggtgca tcttttagaa acgaagcatc gtcatagaac
    10201 acttttccgt ctaatttgga agccggataa cccaacgtta catcgtttga gaagtttacg
    10261 ttttcgctca aggctttggt aatgcgttct aatcggtcgc tttgcgtggt ttttttccag
    10321 gttttcatgt taaatgttga tagcgttaat ttctgcttta tagaaatcta attgttggtg
    10381 cattgtgtct tgattgtttt tgaagaacgc cgtatttttc agtaagttat gataatattc
    10441 aatccctaaa ttgatgttgt ttttataggc attccatttt tttaattgat tggccgattt
    10501 ctcttcggta aaagtacaaa tctcgttttt tagataatcg acatacattt ttaattcttt
    10561 cacaaaaagg tttgggcggt tttcttgcag cattacattg gcgtttccat agatgtgttg
    10621 taccatttct tttagtgaat attcggcatc aaaataagcc aagtttggtc ctggacaaat
    10681 gacaacgcct tgttgttgtc cttttatagg catttcgtgt tctaaataag aagcatttgc
    10741 caaaccgaca caaagacacg atttttcggt tatcttttgt tgtttgattt caaaatcgga
    10801 agtagagatt tcgctttttt gttggtttag tttctctagt tcggcatctt gatatttttt
    10861 ggaagcagta caaattcctt ctttattata ggatttgttt aatgccaaaa actttttcgg
    10921 acacgagctt ccggctttgt tttcgttgat tcttttttgt ttccaaaaat cgtttgtaac
    10981 gccttttagc gagttgaacg gaacgcccaa aggcgaaata ttgctcaaat agaaatcgtc
    11041 ttctttggca tttgccaaca gtttgcgggt ttcggcatcc accgatgtag cttcgggcac
    11101 taaaagaaac ggcgaacccc aaccaatgga atctagctgg tattcttcta ataaaaaatt
    11161 gtgctcttcg gcagttccta cgccaccttg tgcgctgatt tttattggca tactgttttc
    11221 gggcaaaggc aattctttta tagtcaatgc ctttatcaat aaatcgtggg tagattggag
    11281 taactcctgt cttttttctt tgaattcttg caagataggt cccattagaa aaccttcggt
    11341 ggcaaaggca tgtccgccac agtttaaacc tgattcgatg cggtattcgg aaacccaaag
    11401 accttttttg gctaaaaaat tcccttgaat taaagccgaa cgataatcgc ttacttttag
    11461 aatgattttc ttttttaaga aaccattttc atcgggaaaa aagtctggaa agctttcaaa
    11521 atagctgtac aatcttgggt tcattcctgc cgacaacact acggaagact taaggttgct
    11581 gttggcaaat cctctcaaag aagcatgcgc atcgttaaat tcagtgggca attgcacatc
    11641 gccttcataa ttatccttat ctacttttgt catgatgtta acatcgattt cgcccaaaga
    11701 aaggtttttt tcgatgtaat taatcaaggt gtctttcatg gaaataccac tatcaatgaa
    11761 ctcttgcaag ccttttttca agtcggattt attgggtaaa agggcaatgt agttttctaa
    11821 agttgttttg ctctcggcca attctttttt gaagttttcg aatttttcgt tcacaatgct
    11881 atccatcgtg ttgagatagt ttgtgattct ttttgcacga taatcttgaa tcttctgtgt
    11941 aatctcttga taaggaatac tgaacttttc gctgtaaaag gcacgcattt tttcggctaa
    12001 atcatcgtca ataatagaaa ccgttgacga tattccatag tgcgccactc gaatagggct
    12061 atcaattgta aacgccaagc ccatcacggg aatgtgaaaa gaatgactgt tttttttgtt
    12121 tgacatatag ttattttaaa tgtttttaaa tttattttgt tggaaggttt ttattaattg
    12181 aagcataata attcccaatc cggtaataat tcccaatcca aatagaacaa aagcaatgtg
    12241 ccaaccgtgt tgcgattcct gaaatttgct aaaggtttca gatgaatttc caaagagcca
    12301 aaagcttttt ttcattccca taatcaaaag cgttgtccaa aacaggaaaa acgaaatatg
    12361 gaaaatttgg atgccctttt taagcttttg gattgttctt tcagaaaaag taaaggcggt
    12421 attaaagata taggttatgg atgaaaatag gattaaagtg ttgataccga tggttgttcc
    12481 catggaatgt gctacggtaa tgtgtgtgcc gtgtgtgaat aaattaatgg ctggaatgga
    12541 aattaataac gccacaataa tgtttagaaa cacccataaa tcggctaaaa tcataaattt
    12601 ataagcaata gaaaaatgca cctttttttc ttgcaaaaga ttttttctcc agtcataaat
    12661 tatagagaat aggataatcc attcggtcat gctgattatg taagcaaaat agcgaatcca
    12721 cggtgctgtt ggtaccatat aaatgtgatg tgcccagccg aacattaaat tggttaagcc
    12781 taaaaagtag aaaaaaaatg ctttttttga tatggcatat cgactttcgt cacttatttt
    12841 ctccatgaca aaaagtgctg ttccataaac gagcatgttc caagagccaa catacgaacc
    12901 gccggctttc cattgtatcg caacattttg aatgaaatta tctctaaaat agggaaggag
    12961 ccaaaggtgt gcctcggtaa aatgaaaaat catcaataca attccagtgg accacatata
    13021 ataatacaca ggccaagttt tgaacgaaga aaacattgat ttgaaataat tgattccaaa
    13081 taaaatccat cctaaaacaa tgggaaaata aaagtaactg ggaaactcta ggtattcttt
    13141 accagcaaag tttttggaga cataagaaaa agcaatgcca attccggtta aaataaagag
    13201 ccaaaaatgt gctttgacca gcgtattgct ataaaattta aggtttaagt ttgattttaa
    13261 ataataataa ataccaccaa tggcggctaa taaaatccaa gaaacgacaa ataaagtgtg
    13321 caacggtctg atagcgttaa acggaagttt ttctttgatg aaatcgggga aaatatattg
    13381 caaaccgccc aataaaccga aagacattcc taaaaacaag gaaattaaag cggtgattat
    13441 aaaataaaaa ggatacttgt tattttccat tttcaagtaa tttgttttta atggttaccc
    13501 aaccatcttt cccgattgtt gcattggcat ctggataatg cccagtttga tcaatttctt
    13561 taaaaaattg tacgagttgg tctttttctt cttccgagaa attgaattta ggcatggact
    13621 tgatgccgct atttacgatc gttttgatgt atttttcacc ctttccctct ctagaatata
    13681 cattagtaat atcgggacca aggtaaccac ccaaaccata aatttgatgg catgaattgc
    13741 agttgttttg aagccaaatg ttttcacctt ttagtgcttt ttgagacaaa tgaattgttt
    13801 tgtattcaga ttttgaagtg taaataatga agttgtaaat agaaaaaaca caaataaata
    13861 ccaaaataag aatcaaatac tttgaagatt gtgacattgt aatgcattaa ttttcaatat
    13921 tgataactgc tattaattct atttcacttt tgatagaatt ggagattaaa caagctgctt
    13981 ccgatttttc taatatgcgt tgtgctcgtt ccattttatc aagttcagct atgattaact
    14041 cgggttttag aataattttt gtcattaaat attttccatc aattttttct aaaacacctt
    14101 ctgcattaca agaaaatttg atgtattcta attttgaata ttcagcaatg gctaaaaaag
    14161 tagtcataaa gcaactcacc accgaagcag taaataaatg ttcgggcgac caaatgtttg
    14221 gcattccttt gtcaaattcg ggtggtgttg ctacttctat ttttgttgct aattcaggtg
    14281 aagacataat tcctttacgg tcgttttccc aaatcaaatt tacggtatat gtgtgctttt
    14341 ccatgatagg tttttttaag caacaaattg aagcaatttt agagtttata actatgactt
    14401 taatcatgtt attctttttc aattaaaaaa aagcatagac aatggcagcc tatgcttttt
    14461 gaaatcaata aaagactagt gattttgaat taataattac tcttttattt tggcaataag
    14521 acatctccaa taacgtgaat tattccgttt gatgttggga ttgaagctac aatttcggag
    14581 ccatttacaa aagttttatc tccttgtttt gtgattttta cttttccgcc atacaccatg
    14641 tcaaactctt gaccgtcttg catgtattcg gctttaagat ttccaacata ggtgtgatag
    14701 cccaaaatgt tttctaaatc ggcttttttc tctggtttta aaagaccttc aactgtgcct
    14761 gctggcaatt tatcaaaagc ggcgttggta ggcgcaaaaa ccgtaaatgg accggcatta
    14821 ctaagcgaag ttaccaagcc agcggctttt acggcggcaa ctaaagtagt gtgatcttta
    14881 ctgcccgaag ctacttgaac gatgtttggg tttgaaactt catcttgaac accttcttga
    14941 ccaacactta aatcaacgtt gctttcggta gtatttgtct cttttgttgc ttcggtattt
    15001 tctttgcaac ttgtggttag aattaatgaa gcaatagcta agaataaaat tgattttttc
    15061 ataataatta attacttggt ttatagcaac aaagtactta actattagag ttttatttta
    15121 tgattgtact cataaattag cattaatttt aacttttttt atggagaaat aactcgtgat
    15181 gattattcct aaaaacaata gtatggcaat gccaaaaagg atttcgttaa tgaatggaat
    15241 taatgtatag ctaaaggaag caatgccttg aatggctaag gctagttcgt ttagaaagat
    15301 gccaactgta aaaagaataa tgccaaattt taattttttg tgcatataaa aagtatgttt
    15361 ggcatagatg taaaacaaaa gaaataagga tataattgcc aacaatacta aatgtaaata
    15421 agcaatcaca atgggtctaa agccaaacgc caattgactt aatgcaggaa tcgtggagcc
    15481 tagttgcaat aaaaatttaa tacttaccgc taagccaata aagcctaaaa tataacgcaa
    15541 aaagattgct tggtttttaa taaactctaa tttggttttg aggattatta ctaaaacccg
    15601 aaaccatgca tagacttgaa taaaagcggc aattatggtt aagaaataaa tccaattggg
    15661 tagatttaac caaagtgttg agaggaaata agccggaata caggaaacaa aaaacagttt
    15721 agatgcttgt tttaaaaaag gatttaatgc cgaagttaag tttaaaaaag aaaacaacaa
    15781 tcccatgcat gcaaagaaaa accagccatt gtattgaaaa tgtaaataat aataaattga
    15841 agctagatat tcttcttgat gaatgttttt ggtcatcatc atataggcta aaaagaacgt
    15901 gccaaaggaa gaaatgacac taaagaaaac agcagcttta aaccagtttt ttgataaatc
    15961 atcagagtca attcgtttta aatcattgta aaaataaacc gcaaaaacat aagaaacaaa
    16021 gatggaagcg gtggaaaaaa tgattgatat tgctgtataa ccacttataa aaaaggaaaa
    16081 cagcatgcca taggaacaaa ctaaatttaa tataagtaga agaatatatt ttttagaatt
    16141 aataagcact tttgtggtta aataactaat cattactacc atcaatgtgt gtgtaatcca
    16201 tccagaaaaa gcaaaatgtg aatgcgaatg ttgtatgttt ttttgattaa aaagtgaaaa
    16261 ttcaaatcca attttatagc gcattactaa acctaataaa gcgactatag ataagttgat
    16321 gagtgaaaat tttatccaaa acttggtgtc aaatggcatt ttaataatat attttatgtt
    16381 taataatatc tacttttttg caggttttta attttgaaaa tacacgaata acggtttcta
    16441 cccttaaacc cgtaaaattt gcaatttctt gtcgcgtaaa aggcactaat tctttttgat
    16501 ttgtttccga tgtttttttg atagagttta aaaatgaaag tactctaaac tcaggttttt
    16561 gattgataat atctttggca gtgttggctt ttgaatgtat gcggctagct aaagttttta
    16621 ggaatttttt ctgaatttga ggatattcat ccattatttt caaaaactta tctttagaaa
    16681 gtttgatgat tttgctttct tgacaagcta aagctgtagc tggatatgcc tcgtcgatga
    16741 aaagtggtgg ttctccaaag ctttgtccgt tagagaaata gccttgcgta aattcttttc
    16801 cttcatcgtt tgagttaaac atttttacgc ttccttcaat aatttgatag taaaaatttg
    16861 ccatttcatc ttcaaaaaaa acagtttcgt ttttcttgta aactttggct attgcacccc
    16921 aagtaaaaag tatgtccaag tcaatttgca tatatttttt tatgtttttg tatcatttga
    16981 acctgttttt ggaagtatta agcaaaatta agcttagtta tttgcataaa atatgattaa
    17041 aatcatgttg tgcttttgtg aatgttttta aatttgcctc atgaaaagtc ttataatgat
    17101 gatggcgcta accatgcttc taaaaccagt ttttccggtt gtagaataca ttgtcaatta
    17161 tgattatata gcaaaagtac tttgtgaaaa caaaacgaaa cccgaactaa aatgtaacgg
    17221 taaatgtcat ttaatgaaag aaatggcaaa acaagctgaa aacgaaaagc caattcaaaa
    17281 taataaaaaa caagccactt cggagtttga agttttgttt tttcaagaaa ttgcttcatt
    17341 acaatttaca caaagtaatg gtgatgataa atcaataacc ttagattgct attctaatca
    17401 ttactttcat ttgaatagtg atgcagtttt tcgtcctcca atttttagtt gttaaacata
    17461 gttattcaat cattttatta agatgttgaa taggatttta tatccaaaaa attgcttgaa
    17521 aaacattcaa gtaaaaatct cttatgcttt aatttaaaat aaaaatgaaa aatcaattaa
    17581 aaaaaatagc tttagtattg attacactag cttttgcatc atgttctaat gatgataatc
    17641 aaacaaatac aaatgaatta gacgggttga ccaaatttaa agaagtggtt gaaggcaatc
    17701 aaactataga gttttattcg ccaaaaggtg ctttatcaca aggttataat gacataaaat
    17761 ttagaattaa aaacaacgaa accaatcaat atatcacgga tgcgcaagtg gagtggatgc
    17821 caaaaatgca catgacgatg atgacacact cttgtccgaa atcggaagtt gtaaaagcag
    17881 caactggaaa tttatatgaa ggctatatag tgtttcaaat ggcacaaaac gaaacagaat
    17941 attgggattt aactttagat tactctattg atggagaaga gcattcttta atcattccga
    18001 ttgatgttcc tgcaactgca aaacgtgttg tgagtagttt tatgggtagc gacaacgttc
    18061 gatacgttat tgcttatatc gatccaacta gtccgaaagt agggatgaat gatttaaaag
    18121 ttgggatttg gaaaatgcaa gacatgatga cttttccaat gataaatgac tatactgtaa
    18181 aaattgaccc gagaatgcca agcatgggga atcacagttc gccaaataat gtaaatgcac
    18241 aacaaacaac tgttgacaat ttttatattg gcaaattatc gttaaccatg acaggttatt
    18301 ggaaaattaa tttacagctt caaaatgatt taggaaccat cgttaaaggt gaagaaatta
    18361 cagattcagt taccgaaagt agcctctttt ttgaaataga attttaatag taaacaaaaa
    18421 ggtcaataat acttttttgt tggccttttt caaattcaaa aaaaggaatt atgaaggaaa
    18481 tactaagttt tctgttggtt ttctgtggtt ttaattctgt tatttcacaa gaaacccatc
    18541 aagataccat aagcccaaaa cagttgaaag aaataattgt tattggcaaa aaaacgcaag
    18601 ttttcgaaaa gcaaaccaaa tcattgtcaa ccgtggatga atatttgcaa aaatcatcta
    18661 gagtaggtat gataaaacgt ggtgcttatg cttgg
//
LOCUS       DAOOBH010000035        18439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_4001_length_18439_cov_5.308584, whole genome shotgun sequence.
ACCESSION   DAOOBH010000035 DAOOBH010000000
VERSION     DAOOBH010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 18439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18439
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4001_length_18439_cov_5.308584"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..457)
                     /locus_tag="PK218_05480"
     CDS             complement(11..457)
                     /locus_tag="PK218_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002662405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW97993.1"
                     /translation="MQKEFLTITLADDDEDDRLFFQDAFEELKINTNVTTCNDGVELM
                     EYLSNPDTILPNILFLDLNMPRKNGFECLLEIKKESRFDNIAIAIYSTSSSEEDIENT
                     FVHGANIYIKKPSDFATLKKVLSEVVTLNWQYHTSGLNKENFLLRL"
     gene            complement(470..1030)
                     /locus_tag="PK218_05485"
     CDS             complement(470..1030)
                     /locus_tag="PK218_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPW97994.1"
                     /translation="MKTFIKFDINIVTKKLLEDLLKPFDLKFRVLGFGEIEFLENVSE
                     EKINQITAVLNEYGIEIIENQKSILVQKIKDVITEMVNSDEPIAVKSSVFLADKLNHS
                     YGYLSNLFSEVTYTSIENYIIIQKIEVAKNLIINNQLTLTEIAFKLNYSSVAHLSTQF
                     KNITGITPSAFQRIVKKRREISIKTL"
     gene            complement(1142..1873)
                     /locus_tag="PK218_05490"
     CDS             complement(1142..1873)
                     /locus_tag="PK218_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPW97995.1"
                     /translation="MENTNYKQRLGLFVFIGLIFFVGIIFLIGRQQNLFSAKIKISTA
                     FKNASGLKVGNAVRFSGIAIGTVDNIEIVNDSTVKVEMNIKNDTQKFIKTDSKASITS
                     EGVIGDKILVISQGSSTSKSISDGQKIGSFEPIEFSDVLANVKVSAENAEVITHELAT
                     ILTNINDGEGTLGKLMTDEDMAEDLDATMENLRKSSKGLNENMEAAKHNFLLRGYFKK
                     KEREKRKAAEKAKKEAEKKQDNQKK"
     gene            complement(1881..2624)
                     /locus_tag="PK218_05495"
     CDS             complement(1881..2624)
                     /locus_tag="PK218_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW97996.1"
                     /translation="MKTNNSKILIDIQNVTKSFGENEVLKGVTMQLYENENLVVLGKS
                     GTGKSVLIKCIVNLLEQDSGLIKVIDLEVTKNDEENLLAIREKIGFLFQGAALYDSMT
                     VRENLAFPLTRSKKKLNVENLETKIIEALENVSLPDAIDKMPSELSGGMKKRIGLART
                     LITNPKILLYDEPTTGLDPITSDEISDLINFTKKKYKTSSIIITHDIKCVKSVADRII
                     MLKEGTVYKEGTLQEFENDQDVYIKSFFN"
     gene            complement(2621..3424)
                     /locus_tag="PK218_05500"
     CDS             complement(2621..3424)
                     /locus_tag="PK218_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW97997.1"
                     /translation="MKKIYSFKSSKSRLSNFREKFNEFLIKIADVVAFLQIIFKNTFS
                     KNFEGQEFLKQCYVIGNKSAGLISITGIIIGLVLTIQSRPPLVDFGAVAMLPGMVAVS
                     LIREMGPVITALICAGKIGSSMGAELGSMRVTEQIDAMEVSNANPIKYLVVPRVLAAT
                     VMIPLLTLYADVVGIFGSWLGVNIKDDVSLTLFINQAFASVEFIDIIPAVIKSFFFGG
                     VIGIVGCYKGYNAERGTESVGVAANSAVVTASLLVIIVDLVAVQITDLL"
     gene            complement(3661..5931)
                     /locus_tag="PK218_05505"
     CDS             complement(3661..5931)
                     /locus_tag="PK218_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPW97998.1"
                     /translation="MKSYRLFLAIGTFFIISSCTGLRAVPEGDLLYTGAKVKIEDKDI
                     ARKEKKAIETEIKELLVPKPNKSILGLRPSLFFYNLAGDVKKEKGFRHWLKYKLGKEP
                     VLFSKVDLDYNSDIIQNYIENIGYFNATTIADSTKNGKKAKAEYAITLRKQYKIKSIT
                     LPNDSTILSKEINKSMDKTLLKVGKPYNLQYIKNERTRIDIRLKEKGFFYFDENYIFF
                     QVDSTVANHEVDLILKLKNEIPAIAQKQYRINNINIYPNFTINDTLKTSKDSVIVYKD
                     FTIYDKERLFKPKIYDRALYFHKEDLYNRTNHNLSLNRLITLGTFKFVKNQFTTVEGK
                     DGYLDANYYLTPLPRKSIRVEVLAKTNSANYNGSEINVNWSNRNTFRAAELLTISAFG
                     GIEVQISGQNNGFNVYRFGGEANLIWPRFITPFRIESSSGFVPRTKLHLLYEKQIRKQ
                     LYNLNTFKSSFGYLWKENERKEHELKVTEITFVNSTNVTDLYNQQITDNESLAKVIEK
                     QLIFGPTYSYTYTNTMLKQKKHTFYFKGSMDLSGNAAGLLMGANSSTEPKKILDVPFS
                     QFIKIESDFRHYLNLGGELQLASRAIFGLGFAYGNSRELPFIKQFFIGGTNSIRAFRA
                     RSIGPGTFDGNIQNTGFLPDQSGDVKLELNTELRGKIYNFIKGAVFIDAGNIWLLNDN
                     PLKPGSQFNGKFLEQLAIGTGVGLRFDFSFLILRTDLAFPLKYPSSKEFNSINLFDNS
                     WQKNNLVFNLAIGYPF"
     gene            complement(5918..10927)
                     /locus_tag="PK218_05510"
     CDS             complement(5918..10927)
                     /locus_tag="PK218_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPW97999.1"
                     /translation="MEKNKIKKITQRITKILLWIIGSIIGLFLLLVIALQIPVVQNFA
                     KNKAVTYIENKIKTKVNVGKIEIGLPKNIILEEVYIEDQKNDTLLYGKKIIANISLFK
                     LLDNELEINSINLNGINAKVTKNKDGVFNFDYIIDAFASKEKPKKEDKPMLISLSKII
                     LDDIKIAFDDAKSKNDFSMKLNHFDTKIKKFDLENLSFEVPKIKLDGLRLRLNQGIAE
                     ATAKSTKAIKNEVEKKTFQFSNEQVTLSNIDIAYRQENSKFDTKLKFKHLDIEFNTID
                     LLNQKINIKKLSLSETNGNVVLGKIEKKTIQKQTATNSNPWEIEVNNIDLNKVNFSFD
                     DNNAIKKSKGIDYSHLNLTNLKLDANDIKYSQTLSSGIINSFSVSEKSGLVVEEFKTK
                     FYYSSTASYLKNLYIKTPKTEIKDQILANYTNINTLSTDLNQLGVKAVIKKSKIAVSD
                     ILLFAPQLANQKMFISNKNAIVKIDGTVFGKINNLNIPSIEISGIGKTYAAISGKIKG
                     LPDAQKSYYDITIKKIQTTSDDIYSLVSKSTIPENIQLPKTISTKGTFKGSISNFDVN
                     LLVNSSMGNAKVEANFDQTTKDKEKYTAKAWLDEFNLGAFLKNKNLGKLSVQADVKGI
                     GLNPKTANTTVNSTITKAEFNQYAYHDINANATLQNGLYNATINSNDQNLKFALVSNG
                     GFKDKYPTLKLNLKLDIADLRKLNLHAGYLKIKGDVNADFDQIDIDNLNGKLSITNTL
                     VALETEQFPIDSINVIATSTSEMNTIKLQSQFANADINGKYKLSKLQEAFANSISNYY
                     NLNDKKAIKIEPQQVDFLIQLKDEPLIQKMLPELKNLSAIKIDGKYNSVNDTIQIHAV
                     VPKLDYKDNIITNAIFKINKEENALVYNFEVDDIKNNSFHLPYTSVFGKIENNTIDYE
                     LLLKDIKNVERYLLAGTLKNVNGNSEIAIKNDNLKLDYKKWTIDNDNSIRIDKNGVYI
                     NNFILTNGSNKLAINSEKNVSNSPINIEFDDFDLNALTSIVESDFEVGGKIDGTTKIT
                     NINTSPIFIADINIENFAIKKDTVGNLNIKIDNKTANIYNAAVKISGLENSVFLDGNY
                     QVGSSTFDLKLNIEKFQMKSIQAFTLGNLTNSTGFLNGKLDINGNTKQPNINGFIKFN
                     QVGFDVKTLNSKFKLLNDNVAFDNKKMVFTNFKLQDENDNNLVLDGAISIADFSNLGL
                     DLSLVANNFKLINSSEKDNDLFYGELYLDNDISIKGSLNKPIINGDVKINKDTKFSIV
                     LPQTEPSIASREGVVEFIDQDQPILYDKNKDAEKLNQSNITGVEASVNIEVDKDAEIS
                     IIIDKANGDFLKLKGEAELTGGIDASGKTSLTGKYEFTGGSYEMSFNLIKRKFDIKPG
                     SYILWKGEPTLADVNVTAVYKTDVAPIDLVADQLGNVSAETRNTFKQKIPFETELKMN
                     GELLQPKISFDIILPEGNNSVSAEVINTTKAKLDQIRQQEDVMNKQVFAMLLLGRFIG
                     ENPFESEAGGISASTLARESASKILSQQLNNLAGDVISGVELNFDLQSTEDYTSGIKE
                     NKTELNVGLTKKLLNDRLKVTVGSNIGLEGTPRENEKASTIAGDISADYLLTKDGRYK
                     VRAYRKNNYQVALQGQVVETGVTFVITMDYDKFRELFHSPKKDKKVKSKTQKNKSDEK
                     L"
     gene            complement(10994..12106)
                     /locus_tag="PK218_05515"
     CDS             complement(10994..12106)
                     /locus_tag="PK218_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPW98000.1"
                     /translation="METKTNHHIALDFFVVIATVFTAYILQDIIVPLLFSIILSVLLY
                     PIVSFLERKLRFGRILSAIVSVILLCLLIITLFTAIGIQLDEIISKSDSYVSKIESKI
                     TPLIQQAEKSTGIKTKEIIEPDNLKMEKVVKNHFSNITDFLFASGSILGTMVVVPLYM
                     FFFLLYRKFFISFIYSLCEKKCSKEDAKNILTKLYNVQQNYLVGLLTVMFIVGVLNSI
                     GLLLLGIDYPFFFGFLCALLLLIPYIGIIIGSLLPALVALATKDSFWYAIGVIGIFSF
                     IQFIEGNFITPKITGSKVSINAFVSILSIVVFAMLWGTTGMILALPITASLKVLFDHS
                     PRFQIYGFLLGEPTEEFLKSRARRRLKIWKQIRKNK"
     gene            complement(12179..12364)
                     /locus_tag="PK218_05520"
     CDS             complement(12179..12364)
                     /locus_tag="PK218_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HPW98001.1"
                     /translation="MNTTSLKGKWNEVKGKLKQEYADLTDDDLLYEEGKEDEFYGRIQ
                     QKVGKTKDEIKKMIAEI"
     gene            complement(12388..12861)
                     /locus_tag="PK218_05525"
     CDS             complement(12388..12861)
                     /locus_tag="PK218_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HPW98002.1"
                     /translation="MEAKIGLPKKDLTKSIDDLTVLLANEMILYVKTRKFHWNISGNS
                     FMELHKLFEQHYNSLAKTADEVAERISALGGNAIGSMKNFLKHGEIEENTKDSNTQKQ
                     MLDELLEDHQKTMVQIRKAIDKIGDKSNDVVTEDFLTGILKMHENISWQLRKYTN"
     gene            complement(12967..13605)
                     /locus_tag="PK218_05530"
     CDS             complement(12967..13605)
                     /locus_tag="PK218_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPW98003.1"
                     /translation="MKKVTKNLALVMVILATGFASAQDTTNDDRDQMRFGFKAGANFS
                     NVYDEEGQDFVADGKVGVAAGVFAQIPFGKLFGFQPEAIYSQKGFKAKGSVLGFNYDY
                     TRTSTYLDIPLLFQVKPSSYFTIVAGPQFSYLLETKNEFNNTSSTVEEDINGDNYKKN
                     IFGFVIGADANFNNLVLSGRLGWDISKSDADGNSEKPRYKNQVIQVTLGYRF"
     gene            13774..14592
                     /gene="pyrF"
                     /locus_tag="PK218_05535"
     CDS             13774..14592
                     /gene="pyrF"
                     /locus_tag="PK218_05535"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPW98004.1"
                     /translation="MTTQQLIEQIKIKKSFLCIGLDVDLNKIPKHLLELEDPIFEFNK
                     AIIDATHDVCVSYKPNTAFYEAYGLKGWQSLEKTINYINEKHPEIFTIADAKRGDIGN
                     TSTMYAKAFLEDLNFDSVTVAPYMGKDSVEPFLAFENKHTILLALTSNEGAFDFQTLE
                     VNGKEIYKQVLETSKHWKNAHNLMYVVGATKAEYFTEIRKIVPNSFLLVPGVGAQGGN
                     LYDVCSYGMNDDIGLLINSSRGIIYASNKEDFAQKARVEALKLQQEMEEILNSI"
     gene            14592..15392
                     /locus_tag="PK218_05540"
     CDS             14592..15392
                     /locus_tag="PK218_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPW98005.1"
                     /translation="MAKTFTDQLGTSHSFETTPKRIISLVPSQTELLYDLGLEESIVG
                     ITKFCVHPFHFKTTKKIIGGTKKVHFEKIRLLQPDIIICNKEENTKEIVEELSKICPV
                     WVTDISTIEDNLQMITDFGLLFNKRVEAQKWIDKTNHALIDFQNFNKDKPIQKAAYFI
                     WANPYMVAGNNTFINEMLKLNNFENIYANREERYPEVIIQKIRIQGDPDLVLLSSEPF
                     PFTDEHAFELGRFTHHAKVIFVDGEMFSWYGSRLVKAVSYFKTLQEKI"
     gene            complement(15560..16267)
                     /locus_tag="PK218_05545"
     CDS             complement(15560..16267)
                     /locus_tag="PK218_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HPW98006.1"
                     /translation="MKNLFLLFTAFTFMSCAELQQVANQFPELNQTGGLDINAGLKEA
                     LNNGISKQVSKLTATDGFFKNEMVKILLPDELQKVDSALRKVGMGNLADEGLKVLNRA
                     AEDAVKEATPIFVDAVKNMSFTDAKNILMGNNTAATTYLESTTNTALYAKFNPVIKNS
                     FSKVGADKVWSSIITKYNSLPFVKDVNPDLTDYTTNKAMEGVFKMIAVEEQNIRTNLN
                     ARTSTLLQKVFAMQDKK"
     gene            16415..>18439
                     /locus_tag="PK218_05550"
     CDS             16415..>18439
                     /locus_tag="PK218_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPW98007.1"
                     /translation="MEPQTPYIPKNKVRIVTAASLFDGHDAAINIMRRIIQATGVEVI
                     HLGHDRSVEEVVNTAIQEDANAIAMTSYQGGHNEYFKYMYDLLQEKGAGHIKIFGGGG
                     GVILPSEIAELQDYGITRIYAPDDGRAMGLQGMINDLVQQSDYPSPSLVLPKGKDIQS
                     SIQEKEVTTIARLISFAENKHDEFDKLFKSLPLKGLDMPTPVLGITGTGGSGKSSLVD
                     ELVRRFLIDFPEKTIGLISVDPSKRKTGGALLGDRIRMNAINNSRVYMRSLATRQSNL
                     ALSKYVAEAIQVLKAAKYDIIILETSGIGQSDTEILEHSDVSLYVMTPEFGAATQLEK
                     IDMLDFADLVAINKFDKRGALDAIRDVKKQYQRNHNMWDVNPADMPVIGTIASQFNDP
                     GMNTLYKRIMDKIVEKTDAELVSTFEITREMSEKIFVIPPHRTRYLSEIAENNRHYDE
                     VVLTQVEVAQKLYGIFKTICTVADVTSSAVEMPQLLTKSGIDDSKLKRTEDNKVFLEL
                     LLQQFDKVKMDMDAYNWEVILKWNDKVAKYKNPVYSFKVRDKEIKIQTHTESLSHTQI
                     PKVSLPKYQAWGDILKWNLQENVPGEFPFASGLYPFKREGEDPSRMFAGEGGPERTNK
                     RFHYVSAGLPAKRLSTAFDSVTLYGNDPDLRPDIYGKIGNAGVSICCLDDA"
BASE COUNT         5935 a         2959 c         2729 g         6816 t
ORIGIN      
        1 attattctaa tcataagcgc aataaaaagt tttctttatt taatcctgag gtatgatatt
       61 gccaatttaa ggttacaact tcagataaca ctttttttaa agtggcaaaa tcactaggtt
      121 ttttgatgta tatattggca ccatgaacaa atgtattttc aatatcttct tcgcttgaag
      181 aggtagaata aatggcaatg gcaatattgt caaaccgact ttctttctta atttcaagaa
      241 ggcattcaaa accgtttttt cgaggcatat ttaaatctaa aaataaaata ttgggtaaaa
      301 tcgtatcagg attagaaaga tactccatta gttcgacacc atcattacaa gtggtaacat
      361 tggtatttat tttaagttct tcaaaagcat cttgaaaaaa aagtctgtcg tcttcatcat
      421 catcagcaag ggtaatggtt aaaaattctt tttgcatatt tgtttctaat taaagtgttt
      481 ttatagaaat ttctcttctt ttttttacta ttctttgaaa agccgacggt gtgattccag
      541 taatgttttt aaattgtgtg ctcaaatgtg caacactaga ataattaagt ttaaaagcaa
      601 tctcagttaa tgttaattga ttattaatga ttaggttttt tgccacttct attttttgaa
      661 taataatata attttcgata gaagtatagg ttacttcgga aaataaatta gataggtaac
      721 catagctgtg gtttaattta tcagcaagaa aaaccgaact ttttacagca attggttcat
      781 cgctattgac catttctgtg ataacatctt taattttttg aactaaaata cttttttgat
      841 tttcaataat ttcaattcca tattcattca aaacagcagt tatttgatta attttttctt
      901 cggaaacatt ttctaaaaat tcaatttctc caaaacctaa aactctaaat tttaaatcaa
      961 aaggtttgag taaatcttct aataattttt tggtaacaat atttatatca aatttgataa
     1021 atgttttcat aaataagcta tttcttgtaa atttatatat ttatttttag aaaaaaaatt
     1081 aactaattgc caataatttt atttaatttt aagttatcct gaaaatgaaa acttaaataa
     1141 attatttttt ttggttatct tgtttctttt ctgcttcttt ttttgctttc tcggctgctt
     1201 ttcttttttc tctctctttc tttttaaaat agcctctaag aagaaaatta tgttttgctg
     1261 cttccatgtt ttcatttaaa cccttagaac tttttcttaa gttttccata gtagcgtcta
     1321 aatcttcggc catatcttca tctgtcatta gttttcctaa agttccttct ccatcattaa
     1381 tattggttag aatagtagct agttcatgag ttattacttc ggcattttct gcagaaactt
     1441 taacatttgc caaaacatcg ctaaattcta taggttcaaa tgagccaatt ttttgcccat
     1501 cactaatgga ttttgaagta gagcttcctt gagaaatgac caatatttta tcacctataa
     1561 caccttcgga agtgatagat gctttactgt cagttttaat gaatttttgg gtgtcgtttt
     1621 taatgttcat ttctactttt acagtcgaat cgtttacaat ttcaatattg tcaacagttc
     1681 cgatagcaat tccagagaat cgaacagcat tgcctacttt caatccgcta gcatttttaa
     1741 aggctgttga aattttaatt ttagcactaa atagattttg ttgtcttcca attagaaaaa
     1801 tgattccaac aaagaatatt aatccaataa agacaaataa acccaatctt tgtttataat
     1861 ttgtgttttc catattgttt ttaattaaaa aatgatttta tatatacatc ttgatcgttt
     1921 tcaaattctt gtaatgtacc ttctttataa acggtacctt ctttaagcat gataattctg
     1981 tcggcaactg atttaacaca ttttatatcg tgcgtaatta ttatagaaga tgttttgtat
     2041 ttctttttag taaaattaat taaatcacta atttcatctg atgttatagg atctaagcca
     2101 gttgttggct cgtcgtacag taaaattttt ggatttgtta ttaatgttcg tgctaaacca
     2161 attctttttt tcattccacc cgaaagctcc gaaggcattt tatcaatagc atcgggaaga
     2221 ctaacatttt ctagagcttc aataattttt gtttcaaggt tttctacatt taatttcttt
     2281 ttactacgtg ttaatggaaa agctaaattt tctctcacag tcatagaatc atacaaggca
     2341 gcaccttgaa ataggaagcc tattttttct ctaatggcta ataaattttc ttcatcgttt
     2401 ttagtaacct ccaaatcaat tactttaatt agcccagaat cttgctccaa taaattaacg
     2461 atgcatttta tcaatactga tttaccagtt cctgatttgc ctaaaacaac caagttttcg
     2521 ttttcataaa gttgcatagt tacaccttta agcacctcat tttctccaaa gcttttggtt
     2581 acattttgaa tgtctatcaa tatttttgaa ttgtttgttt tcataataaa tcggttattt
     2641 gaacagccac taaatcaacg attattacta atagtgaagc ggttactact gcagaatttg
     2701 cagcaacacc aacactttca gttccacgtt cggcattgta acctttgtag caacctacaa
     2761 ttccgattac tcctccaaaa aagaatgatt ttattaccgc tggaataata tctataaatt
     2821 ctactgaagc aaaagcttga tttataaaaa gagtcaaact cacatcatct ttaatattaa
     2881 caccgagcca actgccaaat attccaacaa catctgcata caaggttaga agtggaatca
     2941 taactgttgc cgccaaaact cgaggaacta ctaaatattt tatgggattt gcattggaaa
     3001 cctccatagc atcgatttgt tcagttactc gcatagaacc tagttctgct cccatacttg
     3061 atccaatttt tccagcacaa attaacgctg tgataacggg tcccatttca cgaattagcg
     3121 aaactgcgac cataccaggt agcattgcaa cggctccaaa atctactaat ggtggtcttg
     3181 attgaatggt taaaaccaac ccaatgataa ttccagtaat tgaaattaac ccagccgatt
     3241 tgttaccaat aacataacac tgttttaaaa attcttgacc ttcaaaattt ttagaaaacg
     3301 tatttttgaa aatgatttgt aaaaacgcaa ctacatctgc aatctttatt aaaaattcat
     3361 tgaacttttc tctaaaattg cttagtctcg atttgctgct tttaaaagag taaatttttt
     3421 tcataattta aaatgaaatt actgataaac aaaactataa cagtttcaat ttttactctt
     3481 atgcaattat cataaaacgt tacaaaatat caatgttgtc ttcgtaaaaa aataatataa
     3541 ctgtggattg gtgaaattat tttaagaaaa gtgattttga atatttttta gttttatttt
     3601 ttatactaat tgatttttgc aaatgcattg catcattttt ttgtgttatg aaataatatt
     3661 ttaaaacgga taaccaatag ctagattaaa tactaaattg tttttttgcc aagaattgtc
     3721 aaataaattt atggaattaa attcttttga agaaggatat tttaatggaa aagccaaatc
     3781 ggttctaaga attaaaaatg aaaaatcaaa tcgtaaccca acaccagttc ctatggccaa
     3841 ctgttccaaa aactttccat taaattgtga acctggtttt aatgggttat catttagcaa
     3901 ccaaatattt ccagcatcta taaaaacagc acctttaata aaattgtaaa tttttcctct
     3961 aagttctgtg ttcaattcta atttcacatc accagattga tcgggaagaa aacctgtgtt
     4021 ttgtatattt ccatcaaatg ttcctggacc aattgatctt gctctaaaag ctcgaatact
     4081 attagttcct ccaataaaaa attgtttaat aaaaggcaat tctctcgaat ttccataagc
     4141 aaaacctaaa ccaaaaatgg ctctactggc caattgcagt tcaccgccta aattcaagta
     4201 atgcctaaaa tctgattcta ttttaataaa ttgactgaat ggaacatcca atattttttt
     4261 aggttcggtt gatgaattgg cacccataag taatccagca gcattgcccg ataaatccat
     4321 gcttccttta aaataaaatg tatgtttttt ttgtttcagc atggtgttgg tataggtata
     4381 ggaataggtt ggtccaaaaa tcaattgttt ttcaatgact ttggctaatg actcattatc
     4441 agtaatttgc tggttgtata aatcggtaac atttgtcgaa ttgacaaaag taatttcggt
     4501 tacttttaat tcatgctctt ttcgttcatt ttctttccac aaataaccaa aagaactttt
     4561 gaaggtattc aaattataga gttgttttct gatttgtttt tcataaagca aatgcagttt
     4621 ggttcttggt acaaaaccac tagatgattc tattctaaaa ggtgtaataa atcttggcca
     4681 aatcaaattg gcttctccac caaatcgata gacattaaag ccgttatttt gtcccgaaat
     4741 ttgtacttca attccaccaa aagcacttat agttaataat tctgccgctc taaaagtgtt
     4801 gcgattactc caattgacat taatttctga accattgtaa ttggctgagt ttgttttggc
     4861 caatacttct acacgaattg attttcttgg taatggcgtt agataataat tagcatctaa
     4921 gtagccatct tttccttcaa cagtggtgaa ttgatttttt acaaacttaa aagttcctaa
     4981 tgtgatcaat ctatttaagg ataaattatg gttggttcga ttgtataaat cttctttgtg
     5041 aaaatataat gctctgtcgt aaatcttggg tttaaaaagt ctttctttat cataaatcgt
     5101 gaaatcttta tagacaataa ccgaatcttt actagttttt aaagtatcat ttatagtaaa
     5161 attgggataa atgttgatgt tgttaattcg atactgtttt tgggcaattg ctggaatttc
     5221 atttttcaat ttcaaaatta aatctacttc atgattagca acagttgaat ctacttggaa
     5281 gaaaatgtag ttttcatcaa aataaaagaa tcctttttct tttaatcgaa tgtctattcg
     5341 ggttctttcg ttttttatgt attgaagatt ataaggtttt ccaactttta ataacgtttt
     5401 gtccatcgat ttattaatct cctttgaaag aattgtagaa tcatttggta acgttatcga
     5461 cttgatttta tattgttttc ggagtgtaat agcatattct gctttggctt ttttaccatt
     5521 ttttgttgaa tcggcaattg ttgtggcatt gaaataccca atattttcga tgtaattttg
     5581 aataatatct gaattataat ctaaatctac tttgctaaaa agtacaggtt cttttccaag
     5641 tttatatttt aaccaatgcc tgaaaccttt ttcttttttt acatcaccag ccaaattgta
     5701 aaaaaacaaa ctgggacgca atcccaaaat ggatttattt ggttttggca ccaataattc
     5761 tttaatttcc gtttcgattg cttttttttc ttttctagca atgtctttgt cttcaatttt
     5821 aacttttgca cctgtataaa gcaaatcgcc ttcgggtaca gctcttaaac cggtacacga
     5881 tgaaataatg aaaaatgttc caattgctaa aaaaagtcta taacttttca tccgacttat
     5941 ttttttgggt tttagacttt acttttttat cttttttggg actatgaaaa agttctctaa
     6001 atttatcgta atccatagta attacaaatg ttacaccagt ttctacaact tgaccttgca
     6061 gtgctacttg ataattgttt tttcgataag ctctaacttt ataacgacca tcttttgtta
     6121 gcaaataatc ggcagaaata tcgccagcaa tggtacttgc tttttcgttt tctcttggag
     6181 ttccttctaa accaatattg cttccaacgg taactttcaa tcggtcgttt aatagttttt
     6241 tggtcaaacc tacatttaac tcggttttgt tttctttgat tccagaggta tagtcttctg
     6301 ttgattgtaa atcaaaattc aattcaacac cgctaatgac atcgccagcc aaattattca
     6361 attgttggga taaaatttta ctagcacttt ctcttgctaa agtagaagca cttataccgc
     6421 cagcctcact ttcaaaagga ttttctccaa taaatcgtcc caataaaagc atggcaaaaa
     6481 cttgcttatt catcacatct tcttgctgtc ttatttggtc taattttgct ttggtggtat
     6541 tgataacttc tgccgaaaca ctattgtttc cttcgggcag gatgatgtcg aaactaattt
     6601 tgggttgtaa taattcgcca ttcattttga gttctgtttc aaaaggaatt ttttgcttga
     6661 atgtatttct agtttctgca gaaacgtttc ctaattgatc ggctaccaaa tctattggag
     6721 ctacatcagt cttataaacc gctgtgacgt taacatctgc caatgttggt tctcctttcc
     6781 aaagaatata acttcctggt ttaatatcaa attttctttt aattaaattg aaactcattt
     6841 cataagaacc accagtaaat tcatattttc cagttaaaga agtttttcca gaagcatcaa
     6901 taccaccagt caattcggct tcacctttta actttaagaa atcaccattg gctttgtcta
     6961 taataataga aatttcggca tctttatcaa cctctatatt taccgaagct tcaactccag
     7021 taatgttact ttgatttaat ttctcggcat ctttgttttt gtcatacaaa attggttggt
     7081 cttggtcgat aaattcaaca acaccttcac gagaagcaat tgatggttcg gtttgtggca
     7141 atactatcga aaacttggtg tctttattta tcttgacatc accattaatg atgggtttgt
     7201 tcaaactgcc tttaatgctt atatcgttgt ctaaatacaa ttctccataa aataaatcat
     7261 tgtctttttc ggaagaattt attagtttaa agttatttgc aaccaaagac aaatctaagc
     7321 ccaaatttga aaaatcggct atactaatag ctccatctaa tactaaatta ttgtcatttt
     7381 catcttgtaa tttgaaattt gtgaaaacca ttttcttatt gtcaaaagca acattgtcat
     7441 ttaataattt gaatttagaa ttgagtgttt taacatcgaa gcctacttga ttgaatttta
     7501 taaaaccatt tatatttggt tgttttgtat ttccatttat atctaattta ccgtttaaaa
     7561 aaccagtgct attggttaaa tttccaagag taaacgcttg aatgcttttc atttggaatt
     7621 tctcgatatt caactttaaa tcaaaagtac tagagccaac ttgataattt ccatcaagga
     7681 aaacagaatt ttctaaacct gatattttta cagcagcatt ataaatattg gctgttttat
     7741 tgtcaatttt tatattcaaa ttgccaacag tatctttttt tatagcaaaa ttttcgatgt
     7801 ttatatcggc aataaaaatt ggcgaagtat tgatgtttgt gatttttgtg gtaccatcaa
     7861 tttttccacc aacttcaaaa tcactttcaa caatactggt gagtgcattc aaatcaaaat
     7921 cgtcgaattc gatgtttatt ggtgaatttg aaacattttt ctctgaattt atggccaatt
     7981 tattagaacc atttgttaat ataaaattgt ttatatagac accattttta tcaattctaa
     8041 tgctattatc attgtcaata gtccattttt tatagtcaag ttttaagtta tcatttttga
     8101 ttgcaatttc tgaatttcca ttaacattct taagtgttcc agccaataaa tagcgttcta
     8161 catttttaat atcttttaat aaaagttcgt agtcgatggt gttattttct atttttccaa
     8221 aaacacttgt atagggtaaa tgaaatgaat tattttttat gtcatcaact tcaaaattat
     8281 atactaatgc attttcttct ttattaattt taaaaatagc atttgtaatt atgttatcct
     8341 tgtaatccaa ttttgggaca acagcatgaa tttgaatggt atcgtttaca gaattatatt
     8401 ttccatcaat tttgattgct gatagatttt tcaactctgg aagcattttt tggattaatg
     8461 gctcatcctt caattgaatt aaaaagtcaa cttgttgtgg ttcaattttg attgcttttt
     8521 tatcatttaa attatagtaa tttgaaatcg aatttgcaaa agcctcttga agctttgaaa
     8581 gtttatattt tccgttgatg tctgcatttg caaattgcga ctgcagttta atagtgttca
     8641 tttcagaagt agatgtggct atcacattaa tactatctat tggaaattgt tctgtttcca
     8701 atgcaactaa tgtatttgtg atacttaact ttccgtttaa gttatctatg tctatttggt
     8761 caaaatcagc attaacatca cctttaattt ttaaataacc agcatgaaga tttagttttc
     8821 tcaaatcggc aatatccagt tttaaattca atttcaaagt tggatattta tctttaaaac
     8881 ctccattaga aactaaagcg aactttaggt tttggtcatt agaattaatg gttgcattat
     8941 acaaaccgtt ttgtaatgtc gcattggcat taatatcatg ataagcatat tgattaaatt
     9001 cggcttttgt tatagttgaa ttaaccgttg tatttgctgt ttttggattc aaaccaattc
     9061 ctttaacatc ggcttgaacg gatagttttc ctaaattttt attctttaaa aatgcaccaa
     9121 gattgaattc gtccaaccaa gcttttgcag tatatttttc tttatctttt gtagtttgat
     9181 caaaattagc ttctactttt gcatttccca tcgaactgtt aaccaaaagg tttacgtcaa
     9241 aatttgaaat gctaccttta aaagttcctt tggtactgat agtttttggc aactgaatgt
     9301 tttctggaat tgtacttttt gaaaccaaac tataaatatc gtctgaggtt gtttgaattt
     9361 ttttaatagt gatgtcgtaa tatgattttt gggcatcagg caaacctttt atttttccag
     9421 aaattgccgc atacgttttt ccaattccac tgatttctat gcttggaatg tttaaattat
     9481 ttatttttcc aaatacagtt ccatcaattt ttacaattgc atttttattt gaaataaaca
     9541 ttttttgatt ggccaattga ggtgcaaaaa gtaaaatatc actaaccgca attttacttt
     9601 tcttaatgac ggctttaact ccaagttgat tcaaatcagt agataaagta tttatgttag
     9661 tgtagtttgc taggatttgg tctttgattt ctgtttttgg agttttgatg taaagatttt
     9721 ttaaatacga agctgtagaa gaataataaa atttagtttt aaattcttca acaaccaaac
     9781 cgcttttttc agaaaccgaa aacgaattaa ttatcccaga actcaaggtt tgtgaatatt
     9841 taatatcatt ggcatcaagt tttaaattgg ttaaattcaa atgagaataa tcaattcctt
     9901 ttgacttttt tatagcatta ttgtcatcaa aactaaagtt gactttgttt aaatcaatat
     9961 tgttcacttc aatttcccat ggattactat tcgtagcagt ttgtttttga atggtttttt
    10021 tttcaatttt tcctaaaact acattgccat tggtttctga aagagaaagt tttttaatgt
    10081 ttatcttttg atttagtaaa tcgattgtat taaattcaat atcaagatgt ttgaatttta
    10141 atttagtatc aaattttgag ttttcttgtc tgtaagcaat atcaatatta gaaagtgtta
    10201 cttgttcatt agaaaactga aatgttttct tttctacttc gtttttaata gcttttgttg
    10261 atttagcggt tgcttctgct attccttgat ttaatcgaag tcttaatcca tccagcttta
    10321 tttttggaac ttcaaatgat aaattttcta aatcaaattt cttaattttt gtgtcaaaat
    10381 gatttaattt catcgaaaaa tcgtttttag atttcgcatc atcaaacgct attttaatat
    10441 catcaagaat tatttttgat aatgagatta acattggttt atcctctttc tttggctttt
    10501 cttttgatgc aaatgcatcg ataatgtaat caaaattgaa tactccatct ttatttttgg
    10561 ttacttttgc gttgattccg ttcaagttaa tagaatttat ttcaagttca ttatcaagta
    10621 atttgaagag gctaatattt gcaattattt ttttgccata aagcagtgta tcattttttt
    10681 gatcttcaat ataaacttct tctaagatga tattttttgg taaaccaatt tctatttttc
    10741 caacgttaac tttggttttt attttatttt caatataagt aacggcttta ttttttgcaa
    10801 aattttggac aacaggaatt tgtaatgcaa ttactaacaa caaaaacaat cctattatgg
    10861 agccaataat ccacaataga atttttgtaa ttctttgagt aatttttttt attttatttt
    10921 tttccataca aggaattaaa gtaagtaata attttttaga atttataata agattacttt
    10981 tttattcaaa ttactatttg ttttttctaa tttgtttcca aatctttaat cttctcctag
    11041 ctctactttt taaaaattct tcggttggtt cgcctaataa aaaaccataa atttgaaatc
    11101 ttggtgaatg atcaaacaac acttttaaac ttgccgttat tggtaatgct aaaatcattc
    11161 ctgttgttcc ccaaagcata gcaaaaacca ctatggaaag tatggaaaca aaggcattta
    11221 tactgacttt ggaacctgta atttttggtg taataaagtt tccctctata aattgtataa
    11281 aactaaaaat tccaattacc ccaatggcat accaaaaaga atccttcgtg gctaaggcga
    11341 ctaaagctgg taaaagcgaa ccaataataa ttccgatata gggtattaat aagagtagtg
    11401 cacatagaaa accaaagaaa aaaggataat caatgccgag aagcaatagc ccaattgaat
    11461 ttaaaacacc aacaataaac ataacggtta ataacccaac caagtaattt tgttgtacat
    11521 tataaagctt ggtaagtatg tttttagcat cttctttgga acattttttt tcgcacaaag
    11581 aataaataaa actgataaaa aattttcgat ataaaagaaa gaaaaacatg tataatggta
    11641 ctacaaccat agtgcctaat atgcttcctg aagcaaacag aaaatcggta atatttgaaa
    11701 aatggttttt gactactttt tccattttta aattgtcggg ttcaataatt tcttttgttt
    11761 ttattccagt cgatttttcc gcttgttgaa taagtggtgt tattttgctt tcgatcttag
    11821 aaacataact atcacttttt gaaataattt catccaactg aataccaata gctgtaaaaa
    11881 gtgttatgat taataaacat aacaatatca ccgaaacaat ggctgatagg attctgccaa
    11941 aacgtaattt tcgttctaag aaagatacaa ttggataaag taatacgctc aaaattatag
    12001 agaataatag cggtacaatt atatcctgta gtatatatgc cgtgaaaaca gttgcaataa
    12061 caacaaaaaa atcaagtgca atgtgatggt tcgttttggt ttccatattg gttttggtta
    12121 tttaaaaaaa ggagttttct ataaaataaa aaactccttt ttcttgattg ccaatttatt
    12181 aaatttcagc aatcattttt ttaatttcat ctttggtttt tccaactttt tgttgaattc
    12241 taccataaaa ttcatcttct ttaccttctt cataaagtaa atcatcatcc gttaaatcgg
    12301 catattcttg ttttaatttg cctttaactt cgttccattt tccttttaaa cttgtagtgt
    12361 tcatgatatt aaattttaaa attactatta atttgtatat tttcttaatt gccatgaaat
    12421 attttcatgc attttcagta ttccagttaa aaaatcttca gtcacaacat catttgattt
    12481 atctccaatt ttgtcaatag cttttctaat ttgaaccatt gttttttgat gatcttctaa
    12541 caattcgtct aacatttgtt tttgagtatt tgaatcttta gtattttctt caatttctcc
    12601 atgctttaga aaattcttca tacttccaat ggcatttcct cccaaagcac taattctttc
    12661 ggcaacttca tcggcagttt ttgccaaaga attataatgt tgttcaaata acttgtgcaa
    12721 ttccataaag ctatttcctg aaatgttcca atgaaatttt ctagttttta catatagtat
    12781 catttcattt gctaataata cagttaaatc atcaattgat ttggttaaat ctttttttgg
    12841 taatcctatt ttagcttcca ttttttatgt tgtttttatt attaaatctt tgatacaaag
    12901 gtagattgta cattgttttt atttgttata gcattattta agatttttat acaatttcaa
    12961 tatctattaa aatcgatatc ctaaggttac ttgaattact tgatttttat aacgtggttt
    13021 ctctgaattt ccgtcagcat ccgatttgct aatatcccag cccaatcttc ctgataaaac
    13081 taaattattg aaatttgcat cggcaccaat cacgaatcca aaaatgtttt tcttgtaatt
    13141 atcaccatta atatcttctt caacagtact tgaagtattg ttaaattcat tttttgtttc
    13201 taataaatac gaaaactgtg gaccagcaac gatggtaaaa taacttgaag gttttacttg
    13261 aaacaatagc ggaatgtcta aataggttga tgttcttgta taatcataat tgaatcctaa
    13321 gacgcttcct ttagctttaa atcctttttg ggaatagata gcttctggtt gaaatccaaa
    13381 aagttttcca aaaggaattt gtgcaaatac accagctgca acacctactt taccgtcagc
    13441 tacaaaatct tgaccttctt cgtcataaac attggaaaaa ttggctccag ctttaaaccc
    13501 aaatcgcatc tgatctctat cgtcgttcgt agtgtcttga gcacttgcaa aacctgtggc
    13561 taaaataacc attactaacg ctaaattttt tgttactttt ttcataatat ttttatttaa
    13621 aatttatgtt acaaatatca aaagcaaatc aaccttttgt gttatataaa tttttgaaaa
    13681 gattataaaa tttatagaaa tgcagaatat tttaatttgt aattagtaga aaacttggaa
    13741 acgttgtaac tttgaaaacg cagaaacaac actatgacaa cacaacaact tattgaacaa
    13801 atcaaaatca aaaaatcgtt tctatgtata ggattggatg ttgatttgaa taaaattcca
    13861 aagcatttgt tggaacttga ggatccaatt tttgagttta acaaagccat tattgatgct
    13921 actcatgatg tatgtgtttc ctataaaccc aacactgctt tctacgaagc ctatggattg
    13981 aaaggttggc aatcactcga aaaaacgatt aattacatca acgaaaaaca tcctgaaatc
    14041 ttcaccattg ccgatgcaaa acgtggtgat attggtaata catcaactat gtatgccaaa
    14101 gcttttctgg aggatttaaa ttttgatagc gtaaccgttg cgccttatat ggggaaagac
    14161 tcagtagaac catttttggc ttttgaaaat aaacatacta tactattagc attgacttcc
    14221 aacgaaggag cttttgattt tcaaactctt gaagttaacg gaaaagaaat atataagcaa
    14281 gtgttagaaa cttcaaagca ttggaaaaat gcacataact taatgtatgt agttggtgct
    14341 accaaagccg aatattttac tgagattcga aaaattgttc caaatagttt cctgttagtt
    14401 ccaggagtag gcgcacaagg tggcaacctg tatgatgttt gttcttatgg aatgaacgat
    14461 gatattggtt tactgattaa ttcttctcgt ggcatcattt acgcttcaaa caaagaagat
    14521 tttgctcaaa aggctagggt agaagctttg aaactgcaac aggaaatgga agaaatatta
    14581 aactctattt agtggctaaa acgttcacag atcaactcgg aacttctcat tcttttgaaa
    14641 cgactccaaa gcgaataatt tctctggtac cttcacaaac cgaattatta tacgatttgg
    14701 gcttggaaga aagtattgta gggataacaa aattctgtgt acatcctttt cactttaaaa
    14761 caaccaaaaa aataattgga ggaacaaaga aagtgcactt cgagaaaatc cgtttgttgc
    14821 aacctgatat cattatttgt aacaaagaag aaaatactaa agaaatagtc gaagaacttt
    14881 caaaaatttg tccagtttgg gtaacagata ttagtacgat tgaagacaac cttcaaatga
    14941 taaccgactt tggtttgctg ttcaataaaa gagtggaagc gcaaaagtgg atagacaaaa
    15001 caaatcatgc gctaattgat tttcaaaact tcaacaaaga caaaccaatt caaaaagcag
    15061 cctatttcat ttgggcaaat ccctatatgg ttgcgggtaa caatactttt atcaacgaaa
    15121 tgttgaaatt aaacaatttc gaaaacatct atgctaaccg tgaagaacgt tatccggaag
    15181 taattattca aaaaattcga attcaaggcg acccagattt agttttgcta tcatcagaac
    15241 cgtttccttt tacagacgaa catgcttttg aattgggacg ttttacacat catgccaagg
    15301 tgatttttgt tgatggcgaa atgttttctt ggtatggaag tcgtttggtt aaagcagttt
    15361 cttattttaa aactcttcaa gaaaagatat aaaaaaaagt cgttaaacct ttaattaacg
    15421 actttttaac taaccttaac caaatgtaaa aaaaaccaaa ttttacatta caaaggtaaa
    15481 taatatttac accaaacatt gttaaggaat tgttatactt atataattga ttattaaaaa
    15541 attataataa cattttattt tactttttgt cttgcatggc aaacactttt tgaagcaatg
    15601 ttgatgtcct tgcatttaaa tttgttctga tattttgctc ttcaacggct atcattttga
    15661 aaacaccttc cattgccttg ttggtagtat aatccgttaa atcggggttt acatctttaa
    15721 cgaaaggcaa cgagttgtat ttggtgataa tggaactcca aactttatcg gcacccactt
    15781 tgctgaaaga gtttttaatt accggattga atttggcata caatgccgta ttggtagtac
    15841 tttctaaata agtagtagcc gcagtattat ttcccatcaa gatgtttttg gcatctgtaa
    15901 atgacatatt ttttacagca tccacaaaaa taggtgtagc ttctttaacg gcgtcttctg
    15961 cggctcgatt taagactttt aatccttcat cagccaaatt gcccatacct actttacgca
    16021 aggcactatc tactttttgt aattcatctg gcaaaagaat ttttaccatt tcatttttga
    16081 aaaaaccatc tgtagcggtt agttttgata cttgtttgct aattccgttg tttaaagctt
    16141 cttttaaacc agcgttaatg tctaaaccac ctgtttgatt taattctgga aattgattgg
    16201 caacttgttg taattcagca caactcataa aagtaaaggc tgtaaataat aagaatagat
    16261 ttttcattta tataaattta gttttcaaag ttaaaaatac tgctaaactt tgatgttaat
    16321 ttaagttaaa agttccaaaa attatgccaa ggaaatactg aatatttttt attaaaatcg
    16381 taaattgcgc actcaaaatt atcaaattcg caatatggaa ccacaaactc cttatattcc
    16441 taaaaataaa gttagaatag taacggctgc ttctcttttt gatggtcatg atgcagccat
    16501 caatatcatg cgacgtatca ttcaagctac aggtgtagaa gtcattcacc ttggtcacga
    16561 ccgaagtgta gaagaagttg tcaacacagc cattcaagaa gatgccaatg ccattgccat
    16621 gacgtcttat caaggtggac acaatgagta ttttaagtac atgtatgatt tactgcaaga
    16681 aaaaggagca ggacacatca agatttttgg tggcggtggc ggtgtgattt tgcctagcga
    16741 aattgcagaa ctacaagact atggaataac tcgtatctat gcgccagatg atggtcgtgc
    16801 tatgggatta caaggtatga ttaacgattt ggtacaacaa tcggattatc ctagcccatc
    16861 cttagttctt cccaaaggga aagatattca aagttctatt caagaaaaag aagtaactac
    16921 cattgcacgg ctaatttcat ttgctgaaaa taagcatgat gagtttgata agttatttaa
    16981 atcccttcct ttgaaagggt tggatatgcc tactcctgtt ttgggaatta caggaacagg
    17041 tggttcggga aaatcgtctt tggtggacga attagtacgt cgttttttaa ttgattttcc
    17101 agagaaaacc attggtttaa tttcggtcga tccatccaaa cgtaaaacag gcggtgcatt
    17161 acttggtgac agaattagaa tgaacgccat caacaactcc agagtatata tgcgctcgtt
    17221 ggctacgcgt caatctaatt tggcgttgtc taaatatgta gccgaagcca ttcaagtgtt
    17281 gaaagcagcc aaatatgata ttattatttt ggaaacctct ggaattgggc aaagtgatac
    17341 cgagatttta gaacattcgg atgtttcttt atatgtgatg acgcccgagt ttggtgccgc
    17401 tacgcaatta gagaaaatag atatgttgga ttttgccgat ttagtggcaa ttaacaaatt
    17461 cgacaaacgc ggtgcattgg atgctatccg cgatgttaag aaacaatacc aacgcaatca
    17521 caatatgtgg gatgtaaatc ctgctgacat gccagtaatt ggtaccattg cttctcaatt
    17581 taatgatcca ggaatgaata cgttatacaa acgcatcatg gataaaattg tggagaaaac
    17641 agatgctgaa ttggtttcta cttttgaaat cacgcgagaa atgagcgaga aaatcttcgt
    17701 cattccacca catagaacaa gatatttatc cgagattgcc gagaacaacc gtcattatga
    17761 cgaagtagtt ttaacgcaag tagaagttgc gcaaaagtta tacggtattt tcaaaaccat
    17821 ttgtactgta gctgatgtca catcgagcgc agtcgagatg ccacaattgc ttacaaaatc
    17881 gggtattgat gattctaaat taaaaagaac cgaagacaac aaagtattcc ttgaattgtt
    17941 actacaacaa tttgataaag taaaaatgga catggacgcc tacaattggg aagtcatttt
    18001 gaagtggaac gataaagttg ccaaatacaa aaacccagtt tacagcttta aggtgcgtga
    18061 taaggaaatt aaaatccaaa cacataccga aagtttatca catactcaaa tcccgaaagt
    18121 gtctttgcca aaataccaag cttggggcga cattttaaaa tggaatttac aagaaaatgt
    18181 tccgggtgaa tttccttttg cttccggatt gtatccgttc aagcgcgaag gtgaagatcc
    18241 aagtagaatg tttgctggtg aaggcggacc agagcgtacc aacaaacgtt ttcattatgt
    18301 gagtgcagga ttgcctgcca agcgtttatc aacagcgttt gatagtgtga cgttgtatgg
    18361 taacgatcct gatctgcgtc cagatattta tggaaaaatt ggtaatgctg gagtttcgat
    18421 ttgctgttta gacgatgcc
//
LOCUS       DAOOBH010000036        18076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_4096_length_18076_cov_5.146385, whole genome shotgun sequence.
ACCESSION   DAOOBH010000036 DAOOBH010000000
VERSION     DAOOBH010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 18076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18076
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4096_length_18076_cov_5.146385"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..660)
                     /locus_tag="PK218_05555"
     CDS             complement(172..660)
                     /locus_tag="PK218_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW98008.1"
                     /translation="MEDEKQEIIEMFGVHFEQQYNIAPLAARVLGVLIVDGCKSGLTF
                     EALVEKLKASKSSISTNLNLLQKMNLIDYFTVVGDRKKYFKAAPLSQRLKNYLNLVDS
                     EKLLIERIITYREKNNSCSQENVNLQNSYAYKEHILNVEQLLINSINKFKEIEIKNQS
                     SK"
     gene            893..2299
                     /locus_tag="PK218_05560"
     CDS             893..2299
                     /locus_tag="PK218_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HPW98009.1"
                     /translation="MKFAYKIKLLSLYIFTFIFCSCVNKNNDGEFVAYFGGEIVNPNN
                     PYVLFCKDNIVIDSLKLDSNNRFFIKFDSLAPGMYSFKHEPEYQYVYFDKNDSIMVRV
                     NSRDFDNSVAFCGRGDLKNTFLMDLYLRNEKDKNSMFKVFDYGLEEFTKTIDSSNLAN
                     EKFYQLKKQEIKWSEDFDVFAKAAVNFQYYTKKELYPNVHKIRTGNDVFEQIPSNYYD
                     YRKKINFDNAALENYAPFVMYLSHMLNNMGAINYHNHFTEADLALKTNINKMQIADTL
                     IKNEKLKNIMLDNIAFTYFLEDQNMVNNKAFLESYHKFSTDKSKQNEIAKIGNAIQQL
                     RVGNALPNVILIDSNGNKLDSDSFKHKKTVIYFWTGSLIAHFEAVHKKILKFKQKYPN
                     FEYIAINLNDSQDDWLKILKSYQLTGITQLRCDNFEDIKSKWAINKIHRTIILDENGK
                     IKNAFTNIFDTTFENNFK"
     gene            complement(2369..3025)
                     /gene="fsa"
                     /locus_tag="PK218_05565"
     CDS             complement(2369..3025)
                     /gene="fsa"
                     /locus_tag="PK218_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HPW98010.1"
                     /translation="MKFFIDTANLKEIKEAQELGVLDGVTTNPSLMAKEGIAGKNNIL
                     KHYVDICNIVDGDVSAEVIATDFAGMIKEGEELAELHEQIVVKIPMTKDGIKACKYFS
                     DKGIRTNVTLVFSAGQALLAAKAGASYVSPFLGRLDDISTDGLYLIQEIREIYDNYGF
                     ETEILAASIRHTMHVVNCAKIGADVMTGPLSSILGLLKHPLTDIGLAQFLADYAKGNQ
                     "
     gene            complement(3106..3909)
                     /locus_tag="PK218_05570"
     CDS             complement(3106..3909)
                     /locus_tag="PK218_05570"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW98011.1"
                     /translation="MSKVVLITGGSSGIGKAIGEFLLQKGFTVYGTSRNPENNTNSLF
                     PLLQLDVRKVETIQKTIAEVIQKSGRVDVVINNAGVGITGPIEETPTDEMRNNFETNF
                     FGPIEVIKSVLPQMREQKSGLIINITSIAAYMGLPFRGIYSSSKAALEIITESISMEV
                     KSFGINVVNVAPGDFATNIASGRYHAPVIEGSAYESNYGNTLKQMDEHVDSGSNPNEM
                     AFAIYQIINEKKPKLHYKIGVFMQRFSIILKRILPDRIYEAMLMNHYKL"
     gene            complement(3942..4940)
                     /locus_tag="PK218_05575"
     CDS             complement(3942..4940)
                     /locus_tag="PK218_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HPW98012.1"
                     /translation="MKLSWQIVKFKLKETFAIAYGNYNYREALIVSLKHHDSIGYGEC
                     TAIDYYQINLAEFENILLTIQSKIEAQKIVEPIDFYTLLLNLNLPSFLRSAVDCAYWD
                     LFGKLEKKSFLMLNGINYNKLPESSFTISIDAIDNQLKKINQLDWNTFKVKCNQLDRA
                     TVFSLLNTNKKIALDSNGSFTNEDCLWLENQELTSAFLYLEQPMHPENENYKSLSDSK
                     HSNWMADEDCQSIDDLEKLQSHYKSINIKLVKCGGLTPALKMIETARKLNFKIMIGCM
                     TESSIGISAGAVLAPLCDFADLDGATLIANDIAKGSQIVNGKINLSHEFGLGIRMI"
     gene            complement(5019..5116)
                     /gene="ffs"
                     /locus_tag="PK218_05580"
     ncRNA           complement(5019..5116)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK218_05580"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5202..6251
                     /locus_tag="PK218_05585"
     CDS             5202..6251
                     /locus_tag="PK218_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HPW98013.1"
                     /translation="MRKHNALITILLALTIVGCGDTLKDKENYFSINETVLKPKYKST
                     DQVNLEVVNNKNKTIDSIVYFMNNEKINTTKGNGKFSFGLNGKKLGYQNLKALIYFGG
                     DTISSNKRIELVADNAPVLLNYDIVNTYDHDVDAFTEGFEFYRDTLIESTGQNGKSYL
                     AKIDYKTGKTYKKVALEQQYFGEGITVINDKIYQLTWQNATGFIYNANTLKKIKSFIY
                     DKKVEGWGMTNDGTFIYHSDGTEKIWKMDPETQKLIDYVNVYTNDSKIKSVNELEWID
                     GKIYGNIWQKDAIAVINPETGAVESVLDLSALRSKVTNKEAEVLNGIAYNKKTKTIFV
                     TGKNWNKTFEIRVKN"
     gene            complement(6267..7028)
                     /locus_tag="PK218_05590"
     CDS             complement(6267..7028)
                     /locus_tag="PK218_05590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPW98014.1"
                     /translation="MRTNFILLLFSMSLLSYAQLKPVDYNDESQKLSGFSAIPKKSVN
                     NNAGILILPAWMGIDKNSKEVAQKLQDLGYFAFVADIYGVDKRPTNAKEAGQNAGYYK
                     KNISEYHKRIQLALDQLIKQGANPDKIVVIGYCFGGTGAIEAARTNMKVQGVVSFHGG
                     LGRDETREIVEIKPKVLVLHGADDFYVPKEEVEAFQNEMRKANADWQMNYYSNAVHAF
                     THKDAGSDNSKGAAYNEKADKRSWIAFMDFLKEVL"
     gene            complement(7080..8303)
                     /locus_tag="PK218_05595"
     CDS             complement(7080..8303)
                     /locus_tag="PK218_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPW98015.1"
                     /translation="MKKIYLVLILFLSIAGNAQVSIEQTKHNNDIKLSALPYYSFGKG
                     IGITSPDSLFQFNIRFRMQNRVTYIENDGENGAYDGQIRRLRLRFDGYVGNPKFLYAV
                     QLSFAPGDVGEIHDGENINIIRDAVVFYRPNKHWNIGFGQTKLPGNRQRVNSSGGLQL
                     TDRTINNAKFTIDRDFGFQFHNINEYKDKFSYNFKTAISTGEGRNVTGKSDDGVALTG
                     KIELQPFGAFTKDGTYFEGDVVREKKPKLLISGAFQQNNQAQRTQGQLGSDLFEKRTM
                     KSFFLDGMFKYNGWAAMMSYMSRTTSKNALTYNPEDVTEFNYVYVGHGFDYQASYVTK
                     SNYEFIARYSTQNVGKDIEAYAPNTREYSVGVTKYIWEHTFKLQTEINYDSLKFYNGS
                     TKNNWYLRFQVEIGI"
     gene            complement(8401..9732)
                     /locus_tag="PK218_05600"
     CDS             complement(8401..9732)
                     /locus_tag="PK218_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPW98016.1"
                     /translation="MDFTLLLVIIFLSLIFDYINGFHDAANSIATIVATKVLTPFQAV
                     MWAAFFNFMAYWVFGFGVADTVAKTAHTSSIDLTVILAGVIAAIIWNLLTWWKGIPSS
                     SSHTLIGGFAGAAIAHGFRDVTDPEHFGFKIVNWYKDAKAGELLPSGVFIIILFIVFA
                     PLLGMIISYFLSLWMMYSSKKNIYPKLLTVVLMLLGIWFFASVLNFNVTPEKAMFHNK
                     FWYFISESHNIKWFLVAIIFYSLAIFNLFFSSLSTANAERVLKKMQLISSAAFSLGHG
                     GNDSQKVMGIIAAAVAVYLKTNPTTNMSDGWLDLNVVLPSVKDGVEVAGVMPEWIPLT
                     CYTVIALGTLSGGWKIVKTMGSKITKVTAFEGVVAESAGALTLFSTEHFKIPVSTTHT
                     ITGSIIGVGVTKRVSAVRWGVTVKLLWAWVLTIPVSAVLAGLIYYILKLFL"
     gene            complement(9757..10401)
                     /locus_tag="PK218_05605"
     CDS             complement(9757..10401)
                     /locus_tag="PK218_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPW98017.1"
                     /translation="MTLNNLFQFLVPKDKKFFPLFEEASSNLVLLAEALHEGVNAPKG
                     ERENYFKRVEEIEAVLEEITHKTHLELSRNFITPFDREDIHGLIKSMDNVADFMHGAA
                     SRMRLYQVDKITKSIRKLTEINVEACQLIHVGILQLKDMKDHKAIKDTCKKINKLESK
                     ADSVFDKAVADIFENETDVKNIIKYKEVLAALESASDKCKSVSNVMEQISVKHS"
     gene            10588..12216
                     /locus_tag="PK218_05610"
     CDS             10588..12216
                     /locus_tag="PK218_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPW98018.1"
                     /translation="MDINFNKNEDHNKLLLSDLKQRFAKVKLGGGEKRIAKLHQEGKM
                     SARERIDYLLDAKKESIEIGAFVGDGMYAEHGGCPSGGVVVKIGYISGKQCIVVANDA
                     TVKAGAWFPITGKKNLRAQEIAMENRLPIIYLVDSAGVYLPMQDEIFPDKEHFGRIFR
                     NNAVMSSMGITQIAAVMGSCVAGGAYLPIMSDEAMIVDKTGSIFLAGSYLVKAAIGEN
                     IDNETLGGATTHCEISGVTDYKAKDDKDALDRIKSIVGKIGDYDKAGFNRVKSEKPTL
                     EAKDIYGIIPKSRSDQYDMMEVIKRLVDNSEMDMYKEDYGKSIITCYARIDGWAVGIV
                     ANQRTVSKTKKGEIQFGGVIYSDSADKATRFIANCNQKKIPLVFLQDVTGFMVGSKSE
                     HGGIIKDGAKMVNAVSNSVVPKFTVVIGNSYGAGNYAMCGKAYDPRLIFAWPSAELAV
                     MGGTQAAKVLAQIEAASLKKSGQPVDEKVEQELFDKIKARYDAQVSPYYAAARLWTDA
                     IIDPLETRTWISMGIEAANHAPIDKKFNLGVLQV"
     gene            12256..13065
                     /locus_tag="PK218_05615"
     CDS             12256..13065
                     /locus_tag="PK218_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPW98019.1"
                     /translation="MKEFLDYTLIEYDNFNFKVYTLLEIIVVFFIVKLLLYIIKTGIT
                     KSTRFDVAKKYSVYQLIKYVAYVITAIAFFHIVGFNVSYLLLGSSALLVGLGFGLQNL
                     FSDFISGIILLIDSSVKVDDVIEVDGLVGKVVQIDFRTTMVVTRDDKYIIVPNTDLTK
                     NQLVNWTHNDILSRFEIKVGVSYNSDVNVVIKLLKEATLSHKDVEKKPEPIIRFSDFG
                     ESSLDFTILFWSNEVFRIENIKSDIRIKIFDLFNENTIEIPFPQRVVHSPK"
     gene            13072..14373
                     /locus_tag="PK218_05620"
     CDS             13072..14373
                     /locus_tag="PK218_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HPW98020.1"
                     /translation="MLLKILRFFNLSNGEDDQDVVLENVKNNISFRGSNLWILACAIV
                     IASIGLNVNSSAVVIGAMLISPLMSPIVGAGFSLGIYDFDLLKKSLRNLLNATLIGLI
                     FSTVYFFLSPFKETQSELLSRTSPTIYDVMIAFFGGLVGVIAITRKEKGNPIPGVAIA
                     TALMPPLCTAGYGLSIGNFSYFFGALYLYTINCVFICISTYIIVKYLKYPSFQFIDEA
                     QQKRIKYMITAVVLLLVIPSIYFAYKLLDEKKFNYKVDNFINVEFNSKGYAMVIKNIK
                     TNTSPKKIEVAFLNKKFTNSEVKQLNKSLENYGISNAELIIKQDTTDIRNAILSEINM
                     RESNINQKDVRISQLEKIISDNKYNNTTLFGEIKVLYPQIQSVSFSNHEFYQETDSTQ
                     IIPVMIYQSTKNLKAADAEKMTLWLQKRLNKKQVKVFAEIN"
     gene            14374..16020
                     /locus_tag="PK218_05625"
     CDS             14374..16020
                     /locus_tag="PK218_05625"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPW98021.1"
                     /translation="MSTSLFQPFPTAVIGASLPTKFTFPFYYEPHPLSMLAALALQEH
                     LKTQKEWHHNFGLTPDEEGLEIGKMFGVLVVQNRQNELGYLWAFSGKLAESNQWPHFV
                     PTVYDMLHDDGFFKKEEVVLNDINYKIKLLEENKNYHIALEKLQKTKEQAELEIAEQK
                     KSIKKNKKLRAEKRTESLTLLEEDALNKESQDEGILLKKMTQYWHHKSSAAQEMVAPF
                     EAKLENLKEERKQKSAALQHQLFKEYAFLNQYHELKSLGEIFNNNPPAGAGECAAPKL
                     LHYAFQHQLKPIAMAEFWWGKSPKSEVRKHGQFYPACTGKCEPILKHMLQGIPLEDNP
                     FEQSLAETTELEIIFEDDYLALVNKPAEMLSVPGKQITDSVARRAKKIYPDATGPLVV
                     HRLDMSTSGIMLIAKSTEVYKKLQQQFIKRSIKKRYVAMLDGVLPGEKGEINLPLRVD
                     LDNRPNQLVCYEYGKKAITLWEIIERTATQTRVYFYPITGRTHQLRVHAAHQLGLNTP
                     IVGDDLYGTKAHRLHLHAESITFIHPISKETLTIHCHAPF"
     gene            16354..17119
                     /locus_tag="PK218_05630"
                     /pseudo
     CDS             16354..17119
                     /locus_tag="PK218_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211544.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
     assembly_gap    17120..17129
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17130..17962
                     /locus_tag="PK218_05635"
     CDS             <17130..17962
                     /locus_tag="PK218_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPW98022.1"
                     /translation="VKPMMECFQSKFGEYPFKEDGYKLVETPYLGMEHQSAVAYGNKY
                     RKGYRGLDLSMTGIGLTFDYITIHESGHEWFGNSVTSKDIADMWIHEGFTQYSEIVYV
                     ECQLGYEKAMKYANGLRKNVANDRPIIGNYGVNNEGSGDMYPKGALLLNTLRHVINDD
                     TKWWKMIYDYSQTFRHKIIDTETVIGFFNQESGMNLTPIFNQYLRHTTIPTLEYQVNK
                     KCLEYRWKTNEPDFSMPIDIAINGTKRRIYPETTWTVSKIKVKPDDVVEILKEGFYIK
                     "
BASE COUNT         6169 a         3089 c         2916 g         5892 t
ORIGIN      
        1 aaattgttgg aaattcagca atcaaggtag tatttgcttt tgcaatttca acaattttgt
       61 atggcattgc aactacttct ttcttttctt cagttttttt attgcaagaa aaaagactta
      121 ctgcaaggat taagacgcta atgctattta ttttcttttt catggtgata attatttact
      181 cgattgattt tttatttcta tttctttaaa tttatttatg gaattgatta atagctgctc
      241 tacattaaga atatgttctt tgtaggcata actattttga aggttaacgt tttcctgaga
      301 acaggaattg tttttttctc tataagtaat tatcctttct attaataact tttcactatc
      361 cactagattc aaataatttt ttaatcgctg gcttaaaggt gccgctttaa aatatttctt
      421 tctatcacca acaactgtga agtagtctat taaattcatc ttttgaagca aatttaagtt
      481 tgtggatatt gaactcttac ttgcttttaa cttttcaact aatgcttcaa atgtcaatcc
      541 agatttacaa ccatcaacaa ttaaaactcc taaaacccta gctgctagtg gtgctatgtt
      601 gtattgttgt tcaaaatgaa caccaaacat ttcaattatt tcttgttttt cgtcttccat
      661 atttcaaaat atttttacaa agataatccc agttcgcaaa acaccgaact aaccgaacat
      721 aaagttttgt taaatttgaa aacaaaactg aatatagatg ttggaatgaa aataaattaa
      781 agaataaaag attgcttatc ttcgagattc taaagtcttt ccaactttaa ctaatgctta
      841 acacctatta tcaatacatc tacttaaatt tgtttacaac gaaattattt aaatgaaatt
      901 tgcctataaa ataaaattac tttccctata tatttttact ttcatttttt gttcttgtgt
      961 caataagaat aatgatggtg aatttgttgc ctattttggc ggagaaatag ttaatccaaa
     1021 caatccttat gtattgtttt gcaaagacaa tattgttatt gattctttaa aactggatag
     1081 taataatcgt ttttttataa aatttgattc gcttgcacca ggtatgtatt ccttcaagca
     1141 cgaaccagaa tatcaatatg tatattttga taaaaatgat agcattatgg ttcgcgtaaa
     1201 ttctcgtgat tttgacaatt cggttgcttt ctgtggtcgt ggtgatttaa aaaacacttt
     1261 tttaatggat ttatatttaa gaaatgaaaa agataaaaac tctatgttta aagtgtttga
     1321 ctatggtttg gaagaattca caaaaaccat tgattcttca aatcttgcca atgaaaagtt
     1381 ttatcagtta aaaaaacaag aaataaaatg gagcgaagat tttgatgttt ttgccaaagc
     1441 tgcagttaat tttcaatatt ataccaaaaa agaattgtat cctaatgttc acaaaattag
     1501 gactggtaat gatgttttcg aacagattcc atctaattat tatgactata gaaaaaaaat
     1561 aaattttgat aacgcagcct tagaaaatta tgctccattt gtgatgtatc tttctcatat
     1621 gcttaacaac atgggtgcta tcaattatca caatcatttt actgaagctg atttagcatt
     1681 gaaaaccaac atcaataaaa tgcaaattgc agatactttg ataaaaaatg agaaactaaa
     1741 aaatataatg ctggataata ttgcgtttac ttatttttta gaagatcaaa acatggttaa
     1801 caataaagct tttttagaat cttatcataa attttctact gataaaagta aacaaaacga
     1861 gattgcaaaa attggtaatg ctatacagca actaagggtt ggtaatgcgc ttccaaacgt
     1921 gattttgatt gatagtaatg gaaacaaatt agattcagat agttttaaac acaagaaaac
     1981 tgtaatttat ttttggacag gtagtttaat tgctcatttt gaggctgttc ataagaaaat
     2041 tttaaaattc aaacaaaaat atccaaattt tgaatatata gctataaatc taaatgactc
     2101 acaagacgat tggctgaaga ttcttaaaag ctatcaatta actggaataa cgcaattgcg
     2161 ttgtgataat tttgaagata tcaaatcgaa atgggcaatc aataaaattc atcgaaccat
     2221 tattttggat gaaaacggga aaattaaaaa tgcatttacc aatatttttg acactacttt
     2281 tgaaaataat tttaaataag atttataaaa gagtataaaa aaaacttccc aattggaaag
     2341 ccttttttaa attatatagt aatatcaatt attgatttcc tttggcataa tctgccaaga
     2401 attgtgctaa accaatatct gtcaaaggat gtttcaacaa accaagaata gatgataatg
     2461 gtccagtcat aacatctgct ccaatttttg cacaattaac tacatgcata gtgtgacgaa
     2521 tagatgctgc aagaatttca gtttcaaaac cataattatc ataaatctct cgaatttctt
     2581 gaattaaata caatccatca gtagaaatat cgtctaatct tccaaggaaa ggcgaaacat
     2641 aacttgctcc agcttttgcc gccaataatg cttgacctgc tgaaaatact aaagttacat
     2701 tggttctaat tcctttgtct gaaaaatatt tacacgcttt tattccatct ttggtcattg
     2761 ggatttttac aacgatttgt tcgtgcaatt ctgccaactc ttcgccttct ttgatcattc
     2821 cagcaaaatc tgttgcaatt acttcagcac ttacatcacc atcaacaatg ttacaaatgt
     2881 caacataatg ttttaaaata ttgttttttc cagcaatacc ttcttttgcc atcaacgatg
     2941 gattggtagt aacaccatct aaaacaccta attcttgtgc ttctttaatt tcttttagat
     3001 tggctgtgtc aataaaaaat ttcataatcc gttttttgtt taagtataaa taagttgtgt
     3061 tttttttaag ttttgcaaaa ttacgaaatc aaattcaatt gtcgtttaca atttataatg
     3121 attcatcaac attgcttcgt aaattctatc gggtaaaatt cttttcaaga ttattgaaaa
     3181 acgttgcata aaaacaccaa ttttataatg caattttggt tttttctcgt taatgatttg
     3241 atagatcgca aacgccatct catttggatt actcccacta tcaacatgtt catccatttg
     3301 ctttaaagtg tttccgtagt tgctttcata agcagaacct tcaataactg gcgcatgata
     3361 acgtcctgag gcaatattcg ttgcaaaatc tccaggcgca acattaacca catttattcc
     3421 aaaagatttt acttccatgc ttatgctttc ggtaatgatt tctaaagccg cttttgaaga
     3481 ggaataaatt ccacgaaaag gcaaacccat ataagccgca atagaagtaa tattgataat
     3541 cagacctgat ttttgttcac gcatttgtgg taaaactgat tttatcactt caattggtcc
     3601 aaaaaaatta gtttcgaaat tatttcgcat ttcatcggtt ggtgtttctt caattggacc
     3661 agttatccca acaccagcat tattaatgac aacatctact ctgcctgatt tctgaatgac
     3721 ttctgcaatt gttttttgaa ttgtttccac ttttcgaacg tctaattgta gtaatggaaa
     3781 taatgaatta gtgttattct ctggatttct gcttgttcca taaactgtaa aacctttttg
     3841 aagcaaaaat tctccaattg cttttcctat tccagatgaa ccgcccgtaa ttaatacaac
     3901 tttactcatg gagtattttt taaaaattta gttaaagata cttatatcat gcgaattcct
     3961 aaaccaaatt cgtgacttaa atttattttt ccgtttacaa tttgtgatcc ttttgcaata
     4021 tcatttgcaa tcaatgttgc cccatccaaa tcagcaaaat cgcataatgg tgccaaaact
     4081 gctcctgccg aaatcccaat actagactca gtcatacatc caatcatgat tttaaaattt
     4141 aattttcttg cagtttcaat cattttcaaa gctggtgtta atccgccaca ctttactaat
     4201 ttaatattaa tacttttata atgtgattgc aatttttcta aatcatcaat cgattgacaa
     4261 tcttcatccg ccatccaatt ggaatgtttt gaatctgata atgatttata attttcattt
     4321 tcaggatgca tgggttgttc taaatacaaa aaggctgatg ttagttcttg attttccaac
     4381 caaagacaat cttcgtttgt aaaacttccg ttagagtcta aagcaatttt tttattggta
     4441 ttcaataatg aaaaaacagt ggctctatct agttggttgc attttacttt aaaagtattc
     4501 caatctaatt gatttatttt ttttaactga ttatcaatag catcaatact tattgtgaat
     4561 gacgattcgg gtaatttatt ataattaatc ccgtttaaca ttaaaaaaga ttttttttct
     4621 agttttccaa acaaatccca ataagcacaa tcaactgcag aacgaagaaa gcttggtagg
     4681 ttcaaattca aaagtaaagt ataaaaatca attggttcta caattttttg ggcttctatt
     4741 tttgattgaa tggttagaag aatattttca aattcagcta aattaatttg ataataatca
     4801 atagcagtac attcgccata acctatacta tcatgatgct tcagcgaaac aattaatgct
     4861 tcacggtagt tataatttcc ataagcaata gcaaaggttt ctttgagttt gaattttact
     4921 atttgccatg ataacttcat aaaaaataaa ggtataaaaa aatccgaagg ctactgaaat
     4981 aaattcagca caagatttcg gattacagtt gaagaaaaaa atggcaagcg acctacatcg
     5041 caccgctcaa ccacatacct ttgctatgtt cccatcctgg aggattttgc aggagctggt
     5101 cgtgtaggac ttgccggtgc aaatatacaa tctttttatt tttttgcaat attttaagtg
     5161 cattataaat atatttgtga agtttaaatt ttagaacaaa aatgagaaaa cataacgctt
     5221 taattaccat tttattagcc ttaaccattg taggttgtgg cgacacattg aaagataaag
     5281 aaaattattt tagtattaac gaaaccgttt taaaacctaa atacaaaagc accgaccaag
     5341 taaatttgga agtagtcaac aacaaaaata agactatcga tagtattgtt tattttatga
     5401 acaatgaaaa aataaatact actaaaggaa atggtaaatt ctcttttgga ttaaatggga
     5461 aaaaattagg ctatcaaaat ttgaaagcat taatctattt cggtggcgat actatttctt
     5521 caaacaaaag aatcgaatta gtagctgaca atgctcctgt tcttttgaac tatgatattg
     5581 taaacactta cgatcatgat gtagatgcat ttaccgaagg ttttgagttt tatcgtgaca
     5641 ctttgattga aagcactggt caaaatggaa aatcctatct ggctaaaatt gattataaaa
     5701 ctgggaaaac atataaaaaa gtagctttag aacagcaata tttcggcgaa ggaattactg
     5761 ttattaatga caaaatatac caattgacgt ggcaaaatgc aactggattt atttataatg
     5821 ctaatacatt aaaaaagata aaatcattca tctatgataa aaaagtcgaa ggttggggaa
     5881 tgaccaatga cggtacattt atttatcatt ctgacggaac agagaaaata tggaaaatgg
     5941 atccagaaac acaaaaacta attgattatg ttaatgttta cactaacgat agtaaaataa
     6001 aaagtgtcaa cgaattagaa tggattgacg gaaaaattta tggtaatatt tggcaaaaag
     6061 atgcaatagc tgtaattaat ccagaaactg gtgctgtaga aagtgtatta gatttatcag
     6121 cattacggtc aaaagtaacc aacaaagaag ctgaagtttt aaacggtatt gcttataata
     6181 aaaagacaaa aacgattttt gttactggaa aaaactggaa taaaactttt gagattagag
     6241 ttaaaaatta aatttatcag tcaatattat aacacttctt tcaaaaaatc cataaaagca
     6301 atccatgatc gtttatcagc tttctcattg taagctgctc ctttgctatt atcacttccg
     6361 gcatctttat gagtaaatgc atgaacagca tttgaataat aattcatttg ccagtcggca
     6421 ttagcttttc gcatttcatt ttgaaatgct tccacttctt cttttggaac ataaaaatca
     6481 tctgcaccgt gcagtactaa aactttaggt ttaatttcta cgatttctct tgtttcatct
     6541 ctacctaaac ctccatgaaa cgaaacaacg ccttgtactt tcatgttcgt tcttgctgct
     6601 tcgatagcac cagtaccacc aaaacaataa ccaattacaa caattttatc tggatttgct
     6661 ccttgtttga ttaattgatc gagtgccaat tgaattcttt tatgatattc cgagatattc
     6721 tttttatagt aaccagcgtt ttgcccagct tccttagcgt tagttggacg tttgtcaaca
     6781 ccataaatgt cggctacaaa agcaaaataa cctaaatcct gtaatttttg tgctacttct
     6841 ttagaatttt tgtcaattcc catccaagct ggaagaatta atatacctgc attattgttt
     6901 accgattttt ttggaatagc agaaaaacca ctcaactttt gactttcatc attataatca
     6961 acaggtttta gttgcgcata acttaacaat gacatgctaa atagtagaag tataaaatta
     7021 gttctcatct tttttttata aaaataagaa attaaaattt tatacaatat agacaaatgt
     7081 taaattccaa tttctacttg aaatcttagg taccaattat tttttgtgga accattatag
     7141 aatttcaatg aatcgtagtt tatttctgtt tgaagtttaa atgtatgttc ccaaatgtac
     7201 tttgttacac caactgaata ttcgcgtgta tttggtgcat aagcttcaat atcttttcca
     7261 acattttggg tagaataacg tgctataaac tcatagtttg atttcgttac ataacttgct
     7321 tgatagtcga aaccatgacc tacatataca taattaaact cggttacatc ttcaggatta
     7381 taggtcaatg cattttttga agtcgttctt gacatatagc tcatcattgc tgcccaacca
     7441 ttgtacttaa acattccgtc aagaaaaaag gatttcattg ttcttttctc aaacaaatca
     7501 cttcctaatt gaccttgtgt tctttgtgct tgattatttt gttgaaaagc accagaaatt
     7561 aataattttg gctttttttc tcgaacaacg tcgccttcaa aataagttcc atctttagta
     7621 aaagctccga aaggttgcaa ttctatttta cccgtcaagg ctacaccatc atcagatttg
     7681 ccagtcacat ttcgaccttc acccgtagaa attgctgttt taaaattata ggagaattta
     7741 tctttgtatt cattaatatt gtgaaattga aaaccaaaat ctctatcaat cgtaaactta
     7801 gcattgttta tagtcctatc tgttaattgt aatcctcctg aagaattaac tctttgccta
     7861 tttcctggta actttgtttg accaaaacca atattccaat gcttatttgg tctgtaaaaa
     7921 acaactgcat cacgaataat attaatgttt tcaccatcat gaatttcgcc aacatcacca
     7981 ggtgcaaaag ataattgaac cgcataaaga aacttaggat taccaacata accatcaaaa
     8041 cgtagtctaa gtctgcgaat ttgtccatca taagctccgt tttcaccatc attttcaata
     8101 taggtaacac gattttgcat tctgaatcga atgttaaatt gaaacaagct atcaggagat
     8161 gtaattccaa ttccttttcc aaagctataa tagggtaatg ccgataactt tatgtcatta
     8221 ttatgttttg tttgttcgat tgatacttgt gcgtttccag caatagaaag aaacaagatt
     8281 aatactaagt agattttctt cattttttaa atttacttta aattaaagat ttgataacta
     8341 taccattttt gatataaaaa aagccaacac ttactgttag ctttttaaaa ccgatttttt
     8401 ttataaaaat aattttagta tataataaat taatcctgct aaaactgcag aaactggaat
     8461 ggtaagcacc catgcccaaa gcaatttaac agtaacaccc caacgaactg cagaaactct
     8521 ctttgttaca cctactccaa taattgaacc tgtaatagta tgtgttgtag aaactggaat
     8581 tttgaaatgt tctgttgaga ataacgttaa cgcaccagcc gattccgcta caacaccttc
     8641 aaatgccgta actttagtaa tcttagaacc cattgttttc acaatcttcc atccaccaga
     8701 tagtgttcct aaagcgatta cggtataaca tgttaatgga atccattctg gcataacccc
     8761 tgctacttcg acaccatctt taacagaagg taatactaca tttaaatcaa gccaaccatc
     8821 agacatatta gtcgtaggat ttgtttttaa ataaacagca acagcagctg caataattcc
     8881 cattactttc tgagaatcat ttcctccatg acccaaacta aatgcagcag aagatattaa
     8941 ttgcattttt ttcaaaacac gttcagcatt ggctgttgac aaactactga agaataaatt
     9001 aaagattgct aaactgtaaa agattattgc taccaaaaac catttaatgt tgtgcgattc
     9061 actaataaaa taccaaaact tattatgaaa cattgccttt tcaggagtta cattaaagtt
     9121 taaaactgat gcaaaaaacc aaattcctaa caacatcaaa accactgtta atagctttgg
     9181 atatatattt ttcttagaag aatacatcat ccataacgat aagaaatagg atataatcat
     9241 ccctaaaagt ggagcgaaaa cgataaataa aataataata aaaactccgg aaggtaataa
     9301 ctcacctgcc tttgcatctt tataccaatt gacaatttta aagccaaaat gttcaggatc
     9361 tgttacgtct ctaaatccat gtgcaatggc cgctcctgca aatcctccaa tcaaggtgtg
     9421 tgatgatgat gaaggaatac ctttccacca tgttaataaa ttccagataa tggcggctat
     9481 aactcctgca agtataactg ttaaatcaat actactggtg tgtgccgttt tggcaacggt
     9541 gtcggctact ccaaagccaa aaacccaata tgccataaag ttaaagaaag ctgcccacat
     9601 caccgcttga aacggggtta aaacttttgt tgcaacaata gttgcaatag aattggcagc
     9661 gtcatgaaaa ccattaatat aatcgaatat taaggataaa aatattatta caagaagtaa
     9721 agtaaagtcc ataaaagttg gattttgaag aactatttat gaatgtttaa ctgaaatttg
     9781 ttccattacg ttggaaacac ttttacattt atctgaagct gattctaatg ccgcaagaac
     9841 ttctttgtat ttaataatgt tcttaacgtc agtctcattt tcaaaaatat cagcaactgc
     9901 tttatcaaaa accgaatcgg ctttactttc taatttgttt atttttttac aagtatcttt
     9961 tatagctttg tggtctttca tatctttcaa ttgcaagata cctacatgaa ttaattgaca
    10021 agcttccaca tttatttctg tcagtttacg aatagattta gtaattttat ctacctgata
    10081 caaacgcatt ctactagcag caccatgcat aaaatcggca acgttatcca ttgattttat
    10141 taatccgtga atatcctctc tatcaaaagg agtgataaaa ttacgactta attcaaggtg
    10201 agttttatgg gttatttctt caagaacagc ctctatttct tcaactcttt taaaatagtt
    10261 ttctctttct ccttttggtg catttacacc ttcatgcaat gcttctgcta ataaaactaa
    10321 attggaagag gcttcttcaa aaagaggaaa gaatttttta tctttcggca ctaaaaactg
    10381 gaaaagatta tttaaagtca tacttattta attttggtgc aaatgtatta ctcttatgtt
    10441 aattgaatat taatttacac aactataaaa aactcttatt ttacaatttc ttaacatttg
    10501 atttcgaaac ataattaaaa gactttttta aaattaaaac gatttcgtac attagccttc
    10561 gatttataaa ataaactatt ctttactatg gatattaact tcaataaaaa cgaagatcac
    10621 aataaacttt tattatctga tttaaaacaa cgctttgcca aagtaaaact tggtggagga
    10681 gaaaaacgca ttgccaaact tcatcaagaa ggcaaaatgt cagcccgcga aagaattgat
    10741 tacctgttag atgctaaaaa agagagtatc gaaattggtg cttttgttgg agatggaatg
    10801 tatgccgaac acggtggttg tccttctggt ggtgttgttg taaaaattgg atacatctct
    10861 ggaaaacaat gcattgttgt agccaatgat gccaccgtaa aagctggtgc ttggtttcca
    10921 attacaggta aaaagaattt acgagcacaa gaaattgcta tggaaaaccg tttacccata
    10981 atatatcttg tcgattctgc tggcgtatat ttacctatgc aagatgaaat tttcccagat
    11041 aaagaacatt ttggacgcat ttttagaaat aacgccgtga tgtctagcat gggaataacc
    11101 caaattgctg ctgttatggg aagttgtgtt gctggtggtg cttacttacc aattatgagt
    11161 gacgaagcta tgatagttga caaaacagga agtatattct tggcaggaag ttatttagtt
    11221 aaagccgcta tcggagaaaa tatagacaac gaaacccttg gtggtgccac tacacattgt
    11281 gaaatatcag gggttaccga ttataaagcc aaagacgata aagatgcttt agaccgaata
    11341 aaaagcattg ttggaaaaat tggcgattat gataaagcag gtttcaacag agtaaaaagt
    11401 gaaaaaccga ctttagaggc aaaagacatt tatggtatta tccctaaatc tcgttctgac
    11461 caatacgaca tgatggaagt aatcaaacgt ttggtggaca actccgaaat ggacatgtat
    11521 aaagaagatt atggtaaatc catcattact tgctatgcac gaattgacgg ttgggctgtt
    11581 ggaattgtag ccaatcaacg tacggtttct aaaactaaaa aaggtgaaat acagtttgga
    11641 ggcgtgattt attccgattc tgcagataaa gccactcgat ttatagccaa ctgcaaccaa
    11701 aagaaaattc cattagtttt tcttcaagat gtcaccggat ttatggtagg ttctaaaagt
    11761 gaacatggtg gcattataaa agatggtgcc aaaatggtaa acgctgtttc taactctgta
    11821 gtaccaaaat ttaccgttgt tattgggaat tcctatggtg ctggaaatta tgccatgtgt
    11881 ggaaaagcat acgacccaag attaatattt gcctggccaa gtgccgaact agccgtaatg
    11941 ggtggaacgc aagccgcaaa agtattggca caaattgaag ccgcttctct taaaaaaagc
    12001 ggacaacctg tagatgaaaa agtagaacaa gaactatttg ataaaataaa agcccgttac
    12061 gatgcacaag tttctcctta ttatgctgct gctcgtttat ggactgatgc catcatcgac
    12121 ccattagaaa cccgaacatg gatttcgatg ggaattgaag ccgcaaatca tgctcctatt
    12181 gataaaaaat ttaacttagg tgtcttgcaa gtataatttt ttgtacattc atacagtaat
    12241 aaaaacacaa ttcacttgaa agaattctta gactacactc ttatagaata cgataatttt
    12301 aacttcaaag tttatacttt attggagatt attgttgttt tctttattgt aaaattgctg
    12361 ttatacataa taaaaactgg tattaccaaa agtacccgat ttgatgtagc caaaaaatat
    12421 tcggtctatc aattgataaa atatgttgct tatgttatta cagcaatagc tttttttcac
    12481 attgttggct ttaatgtatc ctacctactt cttgggtcat cggcattatt agtaggcttg
    12541 ggctttgggt tgcaaaattt atttagcgat tttatctcag ggattatcct tctcattgac
    12601 tctagcgtaa aagtagatga tgttattgaa gtggacggat tagtgggcaa agtggttcaa
    12661 attgatttta gaaccactat ggtagttact cgcgatgaca aatacatcat tgttccaaat
    12721 actgatttaa caaaaaatca actagttaac tggacgcaca acgatatttt gtcgcggttt
    12781 gaaataaaag ttggcgtcag ttataattct gatgttaacg tagtgataaa actcctgaaa
    12841 gaggcaactt tgagtcataa agacgtagaa aaaaaaccag aacccatcat ccgttttagc
    12901 gattttggcg aatcttctct cgacttcacc atcttgtttt ggtccaatga agtttttaga
    12961 atcgaaaaca tcaaaagtga tattcgcatt aagatatttg acctttttaa tgaaaatact
    13021 attgaaattc cattcccaca gcgcgttgtt cattcaccaa aataaatcgg tatgctacta
    13081 aaaattttgc ggtttttcaa tctttcgaat ggtgaagacg accaagatgt tgttttagaa
    13141 aatgtaaaaa acaacatttc ctttcgtggc tccaatcttt ggatattggc ttgtgccata
    13201 gtcattgcct ctattggtct aaatgtaaat tcatctgccg tagtaatcgg tgccatgcta
    13261 atttctccat tgatgagtcc aattgttggt gcaggattta gcctcgggat ttatgatttt
    13321 gacttattga aaaagtcatt aagaaattta ttaaacgcaa ccttaatcgg tttaattttc
    13381 tctacagttt acttttttct tagcccattt aaagaaacac aatcggaact actttctaga
    13441 acttcgccaa ccatttatga tgttatgata gccttcttcg gaggtttggt aggtgtaatt
    13501 gctattaccc gaaaagagaa aggaaatcct attccaggtg ttgccattgc taccgctttg
    13561 atgccgccat tgtgcaccgc tggatatggt ttgtccattg gtaattttag ttactttttt
    13621 ggagctttat acctttatac tatcaactgt gtgtttatct gtatttctac ttatatcatc
    13681 gttaaatatt taaaatatcc atcgttccaa tttattgatg aagcacaaca aaagagaatt
    13741 aaatacatga ttactgctgt tgtattactt ttagttattc ctagtattta cttcgcctac
    13801 aaattattag acgaaaagaa atttaactac aaagtagata actttattaa cgtagaattt
    13861 aattctaaag gctatgccat ggtgattaaa aacatcaaaa ccaatactag ccctaagaaa
    13921 attgaagtgg cttttttgaa caaaaagttt accaacagcg aagtaaaaca actcaacaaa
    13981 tcattagaaa attacggaat ctctaatgcc gaattaatca tcaaacaaga tactaccgac
    14041 atccgcaatg ctattttatc cgaaatcaac atgcgcgaat cgaatatcaa tcaaaaagat
    14101 gttcgcatct ctcaattgga aaaaatcata tcggataaca aatacaacaa cacgacacta
    14161 tttggcgaaa taaaagtatt atatcctcaa attcaatcgg tttccttttc caatcacgag
    14221 ttttatcaag aaaccgatag cactcaaata attccggtga tgatttatca aagcaccaaa
    14281 aacctgaaag ctgccgacgc agaaaaaatg acgttgtggt tacaaaaaag attgaacaaa
    14341 aagcaagtca aagtttttgc cgaaatcaat taaatgagca cttccctatt ccaacctttt
    14401 cccactgctg ttataggagc atcgttgcct accaaattta ccttcccgtt ttattacgag
    14461 ccacatcctt taagtatgtt agcggcactg gccttgcaag aacatttgaa aacacaaaaa
    14521 gaatggcacc acaattttgg gcttactcct gatgaagaag gattggaaat cgggaaaatg
    14581 tttggtgtct tggtggttca aaatcggcaa aacgagttag gctatctttg ggcattttcg
    14641 ggaaaactag ccgaaagcaa tcaatggcct cattttgttc ctacggtata tgacatgctg
    14701 cacgacgatg gtttcttcaa gaaagaagaa gtagtgctca acgatattaa ttataagata
    14761 aagttgttgg aagagaacaa aaactatcac attgctttag aaaagcttca aaaaacgaaa
    14821 gagcaagccg aattagaaat tgctgagcaa aaaaaaagta ttaagaaaaa taaaaaactt
    14881 cgtgccgaga aaagaacaga atccttaacc cttttggaag aagatgcatt gaataaagaa
    14941 agtcaagacg aaggcatttt acttaaaaaa atgacccaat attggcacca taaatcctct
    15001 gcagcacaag aaatggttgc tccgtttgaa gcaaagcttg aaaatcttaa agaagaacgc
    15061 aaacaaaaat cggctgcttt acagcatcaa ttatttaagg aatatgcctt tttgaaccaa
    15121 taccatgaat taaaaagttt gggtgaaatt tttaacaaca atccaccggc gggtgctggt
    15181 gaatgtgctg cgcctaagtt acttcattat gcctttcaac accaattgaa acccattgcc
    15241 atggccgaat tctggtgggg taaatcgcca aaatcagaag tgcgcaaaca cggtcagttt
    15301 tatcccgctt gtaccggaaa gtgcgaacct attttaaaac acatgctcca aggtattccg
    15361 cttgaggaca atccttttga acaaagcctt gccgaaacta ctgaactaga aattattttt
    15421 gaagacgatt atttggcatt ggtcaataaa cctgccgaga tgctttcggt tcctggtaaa
    15481 caaatcaccg actcggtagc cagaagagca aaaaaaatat atcccgacgc cactggtccg
    15541 ttagtagtac atcggttaga catgtccacc tcagggataa tgcttattgc taagagcacc
    15601 gaagtttata aaaagttgca acaacaattc attaaacgca gcatcaaaaa aagatatgtt
    15661 gccatgttag acggtgtgct acccggcgaa aagggagaaa tcaatttgcc attgcgggta
    15721 gatttagaca accgaccgaa ccaattggtg tgttatgaat atggcaaaaa agctattacc
    15781 ctttgggaaa ttatagaaag aaccgctact caaactaggg tgtacttcta cccgattact
    15841 ggaagaacac accaactccg tgttcatgcc gcacatcaac ttggacttaa cacacctata
    15901 gttggcgatg atttatatgg aacaaaagcc catcgcttgc atttgcacgc cgagagcatc
    15961 acgtttatcc atcctatcag caaagaaaca ttaaccattc attgtcatgc tcctttctga
    16021 taagtattgt gagccgcttt cttttaaaaa aatagtttct tcggttctct aggtgacgtc
    16081 acccgagcag ttgcagtcct ctaccgttca acgggtggct tcacccgagc agttgtagtc
    16141 ttctacaatt gagtgggtgg tttcacccga gcagttgcag tcttctacaa ttgagtgggt
    16201 ggcttcaccc aagcagttgc agtcgtcacc cattgagttg tagaaaccat cagtggtgtt
    16261 ttagactaat tttattaaca ctgtttaaag aaaaaaaaga tgtaacaaaa catccaatat
    16321 gatacatata aaaattaatt ctatatttat aaaatgaaat cattttttct attcggattg
    16381 ctaagtattg gctggcaact gtctgcccaa gaagttttta caagaaaaga cagcttgcat
    16441 ggtggtatgc gtcaagaacg gagttgtttt gatgtgcaac gctatgattt gaatatcaaa
    16501 gtcaatattg atgaaaaaag catcaaaggt agtaacacca ttagttttaa aatagtggac
    16561 agcaccaaga cgatacaact ggatttattt gaaaatatgc aaatagacag tatcgtgcat
    16621 aggaatacaa aattgatgta ccgaagagaa tgtaatgctg tttttatcga tttttcagaa
    16681 atgctaactc cagaaatgtc cgagcaaagc gttgaagttt tttattctgg aaacccgata
    16741 attgctaaac gcgctccttg ggatggtggt tttgtattta gcaaagatga acaaggaaaa
    16801 ccatgggttg gtgtcgcagt acaaggtaca ggtgccagtt tgtggtatcc cgtaaaagac
    16861 actcaaaccg acgaacccga ccgtggtgct agtattaaag tagcggttcc aaacggattg
    16921 atggatgttt ccaacggacg ttttatgggt agtgaagatt tgaaaaacgg ctatacacga
    16981 tgggattggg aagttaaaaa ccccattaat aactataaca ttaccataaa cattgccgac
    17041 tatacccata ttcatgacaa ctataaagga ttggatttgg attattatgt tttgagagaa
    17101 aacgaagaaa aagctaaaan nnnnnnnnna agtaaagccg atgatggagt gctttcaaag
    17161 taaatttggc gaatatccgt ttaaagaaga cggctataaa ttggttgaaa ctccttattt
    17221 aggtatggaa caccaaagtg ctgtggctta tggtaataaa taccgaaaag gctatcgtgg
    17281 acttgattta tccatgactg gtattggttt aacatttgat tatataacca tccacgaaag
    17341 cggtcacgaa tggtttggca acagcgttac ttccaaagac attgccgata tgtggataca
    17401 cgaaggattt acccaatact ccgaaattgt atatgtcgaa tgtcaattgg ggtatgaaaa
    17461 agccatgaag tatgctaacg gtttgcgtaa gaatgttgcg aacgaccgac ctattattgg
    17521 caactacgga gtgaacaatg aaggatcggg cgatatgtat ccaaaaggtg ctttgttgct
    17581 taatacctta cgacatgtga ttaacgacga caccaaatgg tggaaaatga tttacgacta
    17641 ttctcaaacc ttccgccata aaataataga caccgaaacg gttattggtt tcttcaacca
    17701 agaaagtggt atgaacctca cgcctatttt caatcaatat ttgcgccata ccactattcc
    17761 aaccttagaa tatcaagtaa acaaaaaatg tttagaatac cgctggaaaa cgaacgaacc
    17821 cgatttttct atgccgatag acatcgctat aaatggtact aagagaagaa tttatcccga
    17881 aacgacttgg acagtatcca agattaaagt aaaacctgat gatgtagtag aaatcctaaa
    17941 agaaggtttt tatatcaaat agtcttttga tagttgattt ttaaacaaaa atatactttt
    18001 ttaaatgtta aagcgtatcg tttaattgat aaatttttct aaaaaagggt ttttatctca
    18061 tttataaata gtaaat
//
LOCUS       DAOOBH010000037        17534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_4233_length_17534_cov_5.704617, whole genome shotgun sequence.
ACCESSION   DAOOBH010000037 DAOOBH010000000
VERSION     DAOOBH010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 17534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17534
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4233_length_17534_cov_5.704617"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PK218_05640"
     CDS             complement(<1..150)
                     /locus_tag="PK218_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPW98023.1"
                     /translation="MRENDMLEKDEIFSKVLRAGRRTYFFDVRATKADDYYVTITESK
                     KFTEED"
     gene            complement(221..1978)
                     /locus_tag="PK218_05645"
     CDS             complement(221..1978)
                     /locus_tag="PK218_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW98024.1"
                     /translation="MKELQYLNKYFIKYKYRFLLGIVFTIASQIFSLFTPKLISKSFK
                     AVEDFYQKGLSTAFVKSELIQNILLILLTAVIAGVLRFLMRQSLIVMSRHVEFDLKNE
                     VFKQYERLDQNFYKKNRTGDLMNRISEDVSRVRMYVGPALMYTINTFISFTVVVIYMY
                     NVSPILTLYTLLPLPILSFVIFKLSKKINTRSTSFQQYLSKVSSFTQEIFSGIRVVKA
                     YSLENQYQKNMVALASESKTKSMDLARVQSLFGPLLIALIGISNLIVIYFGGMMYING
                     SIKSIGTIAEFILYVNMLTWPVASIGWVSSLVQEAEASQKRINEFLKIEPEIKNNTIE
                     RTEINGNIAFKNVSFTYEDTNIQALENISFSIKKGETLAILGKTGSGKSSILSLLTRM
                     HDVETGTITIDGNQIDQINLIDLRNSIGIVPQDAFLFSDSIKNNIKFGKEDATDDEII
                     TVAKQAVVHDNIIQFSKKYDTILGERGITLSGGQKQRVSIARAIIKNPEILLLDDCLS
                     AVDTETEEQILNNLFAITKNKTTIIVSHRVSSAKNADKIIILEDGKIIQEGSHNQLLN
                     QVGYYAELYLKQLSEKELN"
     gene            2176..3282
                     /locus_tag="PK218_05650"
     CDS             2176..3282
                     /locus_tag="PK218_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HPW98025.1"
                     /translation="MNIEITNPAELQKMNPVFGQVSFDNHEQIVFCHDKYTGLKAIIG
                     IHNTVMGPALGGTRMWNYANEWEALNDVLRLSRGMTYKSAITGLNLGGGKAVIIGDSK
                     LDKTPEMISKFGEYVNSLSGRYITAEDVGTTTEDMDRIHEVTPFVTGISEEKGGSGNP
                     SPVTAYGVYMGMKAAANYQFGTDNLEGKSVLVQGTGHVGETLISYLRKEGAEVYVTDI
                     HRDKMENMVVKYGAKIYEENDIYSANVDIYSPCALGATINDNTIYKIRAKVIAGAANN
                     QLAVEEVHGKILRERGIAYAPDFLINAGGIINVYGEIVKYSKEEAMRRTENIYNTTLE
                     IFKHADSKNITTQLAAMQIAEERIEKRKKENAAK"
     gene            3396..4301
                     /gene="nusB"
                     /locus_tag="PK218_05655"
     CDS             3396..4301
                     /gene="nusB"
                     /locus_tag="PK218_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPW98026.1"
                     /translation="MQSIYAMHQKNSDDIQKEEKFLYNSIDNILDLYLIMISALIEIQ
                     KKEIQFLETSSKKHLATTEEKKPNKKFVNNMLLHQLTESSSISIALENRKINNWTLND
                     DYIIILLNEVKKSSLYQKYMLDKSNSFDDDKQFIVDLFTVVIAPNEKLYEYLEDHKLT
                     WIDDIPVVNTQILKQLKNLNQGEEFRVSKLYKDEEDKEFVSNLFRKTVLNEAIFSKEY
                     ENKTPNWDTERIAEIDTIIMKMAICEFLKFPSIPVKVTINEYLELAKEYSTPKSSMFI
                     NGILDNLVKEFNQEDKIKKTGRGLM"
     gene            4414..4689
                     /gene="yajC"
                     /locus_tag="PK218_05660"
     CDS             4414..4689
                     /gene="yajC"
                     /locus_tag="PK218_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPW98027.1"
                     /translation="MGNIQQFLPFILMFVVIYFFMIRPQQKKAKQEKEFEAGLKVGDK
                     IITKSGFHGKISELSETSVVMETMSGKLKIERSAISLELTASLNKKA"
     gene            complement(4767..5342)
                     /locus_tag="PK218_05665"
     CDS             complement(4767..5342)
                     /locus_tag="PK218_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPW98028.1"
                     /translation="MAEKHTWNKVNQWAEELGKLREIVHKTELVETNKWGGEVYTVNN
                     KNILGLGGFKNYFTIWFFNGVFLKDTTGVLVNANEGVTKGLRQWRFFSKDDIDEKRIL
                     EYIHEAIENEKAGLSIKPEKKAVMKCAFFENELNSNSELKKALEKLTPFKQKEFWEYM
                     ATAKQEKTKIIRLEKIKPLIMENRGLNDKYR"
     gene            complement(5355..6629)
                     /gene="pepT"
                     /locus_tag="PK218_05670"
     CDS             complement(5355..6629)
                     /gene="pepT"
                     /locus_tag="PK218_05670"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPW98029.1"
                     /translation="MQHIIDRFISYVTIDTESDPNSETTPSTEKQWNLANKLVEELKT
                     IGMKEVTIDKHSYVMATLPSNIKEKVPTIGFVSHFDTTPDFTGKDVKPQIVKNYDGGD
                     IILNKKENIVLSPSYFKDLLQYKGQTLITTNGLTLLGADDKAGITEIVTAMEYLIQNP
                     EIKHGKIRICFTPDEEIGRGADLFDVKKFGAEWAYTMDGSQIGELEYENFNAAGVKIT
                     FKGKSVHPGYAKGKMINSMLIANDFINELPKNETPQETKGYEGFFHVTDIQGSIEETK
                     LEMIIRDHDRKKFIKRKELVHKITKKINKHFAKQFGEDIVICEIKDQYYNMKEKVVPV
                     MFIVDLAEKAMKELGIKPLIKPIRGGTDGCRLSYMGLPCPNIFAGGHNFHGKYEYVPV
                     ESMQKAVDVIVKIAELTATTDFSKSKKTKKKN"
     gene            complement(6681..7148)
                     /locus_tag="PK218_05675"
     CDS             complement(6681..7148)
                     /locus_tag="PK218_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98030.1"
                     /translation="MKKVLFISLLLFIASCKSKKTVSDTKDSKAIRTVTASEVESFKK
                     NRAFELGNRLLETCNTSKFKAFSKDEATENVINNATPERIAATCKKINMRNGKYLGLE
                     LIDITYNPELEEYLFRYMISYQKKLYKRELYVTLNNEDKVSAISTKEVKAKPL"
     gene            complement(7222..9627)
                     /locus_tag="PK218_05680"
     CDS             complement(7222..9627)
                     /locus_tag="PK218_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW98031.1"
                     /translation="MTETLLKTEMTFEDFKAEVLNDYKTARISRECSLLGRKEVLTGK
                     AKFGIFGDGKEVPQLALAKAFQNGDFRSGYYRDQTFMMAIGQLTIEQFFAGLYGHTDL
                     NFDPMSAGRQMGGHFATHSLDENGNWKNLMQQKNSSADISPTAGQMPRLLGLAQASKI
                     YRNVTGLESFTNFTNQGNEVAWGTIGNASSSEGLFFETINAAGVLQVPMVMSVWDDEY
                     GISVHARHQTTKENISEILKGFQRDENNKGYEIFRVNGWDYPTLVETYQKAGAIAREE
                     HVPVLIHVQELTQPQGHSTSGSHERYKNAERLAWETEFDCLTQMRNWLLKNNIATEIE
                     LDSIDEISKNLVLEGKKAAWNAFVSPMKEEQNELVALLNSLANTSPNQVFIQKNATDL
                     AAIKEPIRKELLVTARKTLRLIAGENGQQTLATWIKNYTEKIQPKFSSHLFSQSVKNV
                     LSAKEVLPTYSESSEEVDARLVLRDNFDAIFAKYPETLIFGEDSGNIGDVNQGLEGMQ
                     EKYGELRVADVGIREATILGQGIGLAMRGLRPIAEIQYLDYLLYAIQIMSDDLATLQY
                     RTAGRQKAPLIIRTRGHRLEGIWHSGSPMGMIINAVRGIHVLVPRNMTKAAGFYNTLL
                     QTDEPALVIECLNGYRLKEKMPTNLGDFKTPIGVVETIKTGTDITVVSYGSTLRIVEQ
                     AAAELLNFGIDIEIIDAQSLLPFDINHDCVKSLEKTNRLLVVDEDVPGGASAYILQQI
                     LEVQKGYLHLDSQPQTLAAQAHRPSYGTDGDYFSKPSVEDIFEKIYAIMQEVNPSDFP
                     SLY"
     gene            complement(9799..12648)
                     /locus_tag="PK218_05685"
     CDS             complement(9799..12648)
                     /locus_tag="PK218_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPW98032.1"
                     /translation="MHTPNNKVILTLKLLVFWSLLLLSLNTIAQHNSKLIAEFDSEQK
                     TISVIQELTYFNQSNDTLSYVVLNDWNNAYSNKNSPLAKRFSDEFERGFHLANEKERG
                     ITSNITILDENANFLTWERDANHPDVIQFRLREKLLPNEKKIFKLTYIVKITSDRFTK
                     FGYDEKGTFNIKNWFLTPSRFENNTFIKYDNVNLDDESNALSDFELSIKVPSNLVLTS
                     DLNEINQTAKENQTLYHFKGENRLNFSLFLDEKGEFLSFKNEDIDVVSNLRDSRLTDI
                     QRAIVIDKIVNYVAEQLGHYPHKKITISQVDYERNPFYGLNQLPNFISPFPDEFMYEL
                     KFLKTYLNTYIHNTLQLNQREDNWIYDGIQVYLMMKYIDEFHPDSKMMGSLSKLGILR
                     GFNLISLGFNGQYSYFYMLMARKNLDQPLGSPKDSLIKFNEKIASKYRAGLSLNYLDS
                     YLGQNRVQESIKQFFELNKKQQTTETDFENILKKNSPKRIDWFFDKIINSRDIIDYKI
                     NSFSKTKDSVSLTIKNKTATNVPIPVYGIKKKEIVFKYWVDSIAKDSVYTFPRNDADK
                     IVLNYENEVPEFNLRNNWQSLRGFRLNNRPIKFNFMKDLEDPYYNQILYVPTLEYNLY
                     DGFQPGLRVHNKTILDKPFIFDLNPTFSTKTNNISGKGVVYINQYYRDSKINNIKYLL
                     GGHYLHYAPDAYYTKLNPTISVQFRPKNLRDNRVQSFQIKEIIVNREKSAFLTDQNTE
                     SYQVFNAKYYNGKTEVTHHFNFLGDIQLSKTFGKLAAEMQYRHLFNNNRQVNLRFFAG
                     SFLYNTTTSNFFDFGVDRPSDYLFESDYLGRSETKGLFSQQIIPMDGFFKSKLENRFA
                     NQWITTINASTNIWNWIEIYGDLGMIKNRNLSPKFIHDSGVRLNLVTDYFELYFPVYS
                     SNGWEIGQQNYQERIRFIITFNPDTLIRLFTRKWF"
     gene            complement(12661..13386)
                     /locus_tag="PK218_05690"
     CDS             complement(12661..13386)
                     /locus_tag="PK218_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPW98033.1"
                     /translation="MKNNDFENEDEILQEKLKQKDWNEIRTNDSWAIFKIMSEFVNGY
                     ESMARIGPCVSIFGSARTKPDNKYYLLAEKIAFNISKAGYGVITGGGPGIMEAGNKGA
                     HHGGGTSVGLNIELPFEQHFNPYIDKDKNLNFDYFFVRKVMFVKYAQGFVVMPGGFGT
                     LDELFEAVTLIQTKKIGKFPIILVGSEFWSGLIDWIKSVMIGKEHNASPEDMNLIKIV
                     DTAEEVVEELDRFYKKYNLSPNF"
     gene            complement(13456..14013)
                     /locus_tag="PK218_05695"
     CDS             complement(13456..14013)
                     /locus_tag="PK218_05695"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPW98034.1"
                     /translation="MSNQVTLSTSRLLLKSITPAFIHEAFNTKSKEEIINYFGFNEIG
                     YEHHKLMHEKGMETHSVSLFFFLLIDKETNKPIGECGFHTWTAKHNRAEIFYNIHNNA
                     NKQQGYMTEALTAILEYGFTALKLHRVEALIAAENEASLRLLLRQGFIKEGIMREDYV
                     VDGKNEDSECYSLLKWEWERIKKEI"
     gene            complement(14020..16848)
                     /gene="uvrA"
                     /locus_tag="PK218_05700"
     CDS             complement(14020..16848)
                     /gene="uvrA"
                     /locus_tag="PK218_05700"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPW98035.1"
                     /translation="MEDQEYIEIIGARAHNLKNIDVNIPREKLVVITGLSGSGKSSLA
                     FDTIYAEGQRRYIETFSAYARQFLGGLERPDVDKIDGLSPVIAIEQKTTSKSPRSTVG
                     TITEIYDFLRLLFARAADAYSYNTGEKMVSYSDEQIRDLIIEDFTGKRINILAPVIRA
                     RKGHYAELFQQISKQGFLKVRVNGEIKDIVSGMKLDRYKTHDIEIVIDRLEVNDSVDT
                     KKRLAESINTAMYHGENILMVIEHESKETRFFSRTLMCPTTGISYPNPEPNNFSFNSP
                     KGACDNCNGLGTVHQINEKKIIPNPKLSIKNGGFAPLGEYKSSWMFKQLETIGEKFGF
                     TLTDAIEKIPAEAMDMIMNGGKEKFSVTSKVAGVTKDYKIDFEGISHFIKSQFDEANS
                     TSLKRWAKEYMDEINCPVCEGSRLKKESLYFKINEKNIADLSAMDISELIAWFEDLNK
                     HLSEKQKTIATEVLKEIITRLQFLMDVGLNYLSLSRSSKSLSGGEAQRIRLATQIGSQ
                     LVGVLYILDEPSIGLHQRDNERLINSLENLRDIGNSVLVVEHDKDMIERADYVIDIGP
                     KAGKYGGEIISIGTPKELLKENTITAQFMNGKMEIEVPKTRREGNGKFLKLTGATGNN
                     LKNVSIELPLGKLICVTGVSGSGKSTLINETLYPILNEHFFNAVKKPQPYKKMEGLEH
                     IDKVIDIDQSPIGRTPRSNPATYTDVFSEIRKLYTMTPEAMIRGYKAGRFSFNVKGGR
                     CETCEGSGVRTIEMSFLPDVYVECETCMGKRFNRETLEIRYKGKSISDVLNMTVDEAV
                     DFFEMIPKIYRKVKTIQDVGLGYITLGQQSTTLSGGEAQRIKLATELSKKDTGNTFYI
                     MDEPTTGLHFEDIRVLMDVINALVDKGNSVLIIEHNMDVIKLADYIIDIGMEGGKKGG
                     EVIAKGTPEEIIKNKKSYTAQFLKKELK"
     gene            17029..>17534
                     /locus_tag="PK218_05705"
     CDS             17029..>17534
                     /locus_tag="PK218_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98036.1"
                     /translation="MKLLGIGSRLKHAEHGVGVVTNVTSKHYWVTFIENGLETIDIDS
                     DFEVIEALEDEVDSVSFYEVEKTLKNLLMRWSDISPNVAIADKWKGGKLIMEPGTPTQ
                     NKEVPIDTFFHKIVMVRDRVRVMEQKINSSNLEEADKIDLQQYITRIYGSLTTFNVLF
                     KAPSDYFVG"
BASE COUNT         5546 a         2972 c         2800 g         6216 t
ORIGIN      
        1 atcttcttct gtaaactttt tactctcagt aattgttaca taataatcat cagctttggt
       61 agctctcaca tcgaagaaat aggttcttct tccagctctt aaaactttag aaaaaatttc
      121 atctttttct aacatatcat tttctctcat aatgtttttt tagtcttatt gggttacagt
      181 aatgctcaaa aatggtaatt tttttgttac gaaacaacaa ttaatttaat tctttttcag
      241 aaagttgttt caagtataat tcggcataat agccaacttg gtttagtaat tgattatgag
      301 aaccttcttg aatgattttc ccatcttcga gaatgattat tttgtctgca tttttagctg
      361 atgaaactct atgactaaca ataatggtag ttttgttttt tgtaatagca aataagttgt
      421 tcaaaatttg ttcttcagtt tctgtatcaa cagccgaaag acaatcatcc aacaacaata
      481 tttcgggatt ttttataatc gctctagcta tcgaaactct ttgtttttgt ccgcccgaaa
      541 gtgtaatacc acgttcgccc aaaatagtat cgtatttttt gctaaattgt atgatgttat
      601 cgtgaactac ggcttgtttc gctacagtta taatttcatc atcggttgca tcttctttac
      661 caaatttaat attatttttt atactatctg aaaataaaaa agcatcttgc ggcactattc
      721 caatgctgtt tcttaaatca attagattga tttggtcaat ttgatttcca tcaatagtaa
      781 ttgttcctgt ttcaacatca tgcattcgtg ttaataacga aagaatggat gattttcctg
      841 aaccagtttt tccgagaata gccaatgttt ctcctttttt aattgaaaac gaaatatttt
      901 caagtgcttg aatattggta tcctcatatg tgaaagaaac atttttaaat gcaatatttc
      961 catttatttc ggttctttca atagtattat tttttatttc tggctcaatt tttaaaaact
     1021 cattaatccg tttttgcgac gcttctgctt cttgaaccaa tgatgaaacc caaccaatag
     1081 aggcaactgg ccaagttaac atgtttacgt ataaaataaa ttcagctatg gttccaatac
     1141 ttttaatact tccattaatg tacatcatgc caccaaaata aatgacaatc aaattgctta
     1201 ttccaatcaa tgcaattaat aaaggtccaa aaagcgattg tactctagct agatccatac
     1261 ttttggtttt gctttcacta gctaatgcaa ccatgttttt ttgatattga ttttctaatg
     1321 aatacgcttt tacaacgcga attccagaaa atatttcttg agtaaaactg gatacttttg
     1381 ataaatattg ttggaatgat gtactacgag tatttatttt cttactcaat ttaaaaataa
     1441 caaacgataa aataggtaaa ggtaaaagtg tataaagtgt caatataggt gaaacattat
     1501 acatatagat aacgacaaca gtaaaactta taaacgtatt gatagtgtac attaatgcag
     1561 gaccaacata cattctaact ctagaaacat cttcactaat gcggttcatt aaatcgcctg
     1621 ttcgattttt tttatagaag ttttggtcta atctttcata ttgtttgaaa acttcatttt
     1681 ttaaatcaaa ttcaacatgt ctcgacatta cgattaaaga ttgccgcatc aaaaaacgta
     1741 aaacaccagc aataacagcg gttagtaata tcaaaagtat attttggata agttctgatt
     1801 taacaaaggc agtactcaaa cctttttgat aaaaatcttc aactgcttta aatgatttac
     1861 taattaattt tggcgtaaat aatgaaaaaa tttgagaggc aatagtaaac acaataccca
     1921 aaagaaatcg gtatttgtat tttataaagt atttgtttag gtattgaagt tctttcatgt
     1981 aaaaagaaga aaagtcattt ttttaaatct ataaatgtta atttttaaac aattgtataa
     2041 ttgcgatata tttaatttat agcgttttta ttaacaattt cataattatt ttgactttaa
     2101 gcaattattc ttgaataata tcataatttt gcatcgcatt ttagagaata taaacagcaa
     2161 ttaatttatt ttaatatgaa tatagaaatt accaatccag ctgagttgca aaaaatgaac
     2221 cctgtttttg gacaagtatc atttgataat cacgaacaaa ttgttttttg tcacgacaaa
     2281 tatacgggtt taaaagcaat aataggtatt cataatacgg ttatgggacc agctttgggt
     2341 ggaacaagaa tgtggaatta tgctaacgaa tgggaagctc taaatgatgt tttaagactt
     2401 tctcgtggca tgacctataa atctgcaatt acgggtttaa atcttggcgg cggaaaagca
     2461 gtcataattg gtgattcaaa acttgataaa accccagaaa tgatatctaa atttggtgaa
     2521 tatgtaaatt cattaagtgg aagatatatt acggcagaag atgttggaac tacgacggaa
     2581 gatatggata gaatacatga agtaactccg tttgtaactg gaatttctga agagaaaggt
     2641 ggttctggaa atccttcgcc agtaaccgct tatggtgttt atatgggaat gaaagctgct
     2701 gcaaactatc aatttggtac agataatctg gaaggtaaaa gtgtcttagt tcaaggaact
     2761 ggtcatgttg gcgaaacatt aattagttat ttgagaaaag aaggagcaga ggtttatgta
     2821 acagatattc accgagataa aatggagaat atggttgtaa aatatggtgc taaaatctat
     2881 gaagaaaatg atatttattc agctaatgtt gatatatatt caccttgtgc attaggagca
     2941 actattaatg acaatacaat ttataaaatt cgagctaaag taattgcagg tgctgccaac
     3001 aatcaattag cggttgaaga agttcacgga aaaattttaa gagaacgtgg cattgcctat
     3061 gcacctgatt tcttaattaa tgctggtgga attatcaatg tttatggaga aatagtaaaa
     3121 tattctaaag aagaagcaat gcgtagaacg gaaaacatct acaatactac attagaaatt
     3181 tttaaacatg ctgattcaaa aaatattaca acgcagcttg ccgcaatgca aatagccgaa
     3241 gaacgaattg aaaaacggaa aaaagaaaac gcagctaaat aatataagtt ttttaaaata
     3301 atactatttt tgcaaagcgg aagatattct ttcgctttgt taatttttta aagttcttac
     3361 aagtgttaaa cagaagacat attcgactaa aggtgatgca atcaatatat gctatgcatc
     3421 aaaagaattc agatgatatt caaaaagaag aaaaatttct ttacaatagt attgacaata
     3481 ttttggattt atatctaatt atgatttcgg cacttattga aattcagaaa aaagaaattc
     3541 aatttctcga aacttctagt aaaaagcatt tggctaccac ggaagaaaaa aaaccaaata
     3601 aaaaatttgt aaacaacatg ctgcttcatc aattgactga aagcagttcc ataagtattg
     3661 cgctagaaaa caggaaaata aacaattgga ctttaaatga cgattacatt attattctgt
     3721 tgaacgaagt taaaaagagt agtttgtatc aaaaatacat gcttgataaa agcaattctt
     3781 ttgatgatga taaacagttt attgtagatt tatttacagt agttattgct ccaaatgaaa
     3841 aactttacga ataccttgaa gatcataaat taacttggat agatgatatt ccagtagtga
     3901 atactcaaat tttaaagcaa ttaaaaaacc tcaatcaagg cgaagaattt agagttagca
     3961 aactttataa ggacgaagaa gataaagaat ttgtgagtaa tttgtttaga aaaacggttt
     4021 taaacgaagc aattttttct aaagaatacg aaaataaaac accaaattgg gacacggaac
     4081 ggattgcaga aattgatacc attattatga aaatggcaat ttgtgaattt ttaaaatttc
     4141 cgtcaatacc agtaaaagta accattaacg aatatttaga acttgcaaaa gaatattcta
     4201 caccaaaaag tagtatgttt ataaatggaa ttttagataa tttggttaaa gaatttaatc
     4261 aagaagataa aattaaaaaa acaggacgag gtttaatgta aaaactaaac ttttagagta
     4321 ctattttttt cctaatgaaa aaaaataaag aaataataaa aactaaaatt aatactaaaa
     4381 gttcctgatt taggaacata aattttaaac actatgggaa acatccaaca atttttgcct
     4441 tttatattaa tgtttgtagt catctatttt tttatgatta gaccacaaca aaagaaagca
     4501 aaacaagaaa aagaatttga agcaggatta aaagttggtg ataaaataat cacaaaaagc
     4561 ggttttcatg gaaaaatatc agaactatcg gaaacatcag tagttatgga aacgatgtcg
     4621 ggaaaattaa agattgaacg ttccgctatt tctctagaat tgacagcatc cttgaataaa
     4681 aaagcataaa ctttttaaaa cttaaaaaaa attccaaatc ccaattttaa agttggagtt
     4741 tggaattttt tattggaatt taatcgttaa cggtatttat cattcaaacc tctattctcc
     4801 atgattagag gttttatttt ttctaatcgt ataatttttg ttttttcttg cttggctgtt
     4861 gccatatatt cccaaaattc tttttgctta aatggtgtta gtttttctaa tgcttttttg
     4921 agttctgaat tactatttaa ttcattttca aagaaagcac atttcataac tgctttcttt
     4981 tcaggtttaa ttgaaagtcc agctttttca ttttcgatag cttcatgaat atactctagg
     5041 attcgttttt catcaatatc atctttcgag aaaaacctcc attggcgcaa tcctttggtc
     5101 acaccttcat tggcattaac taatactcca gttgtatctt ttagaaaaac accgttgaaa
     5161 aaccaaatgg taaaataatt cttaaaacca ccaagaccaa gtatattttt attgtttaca
     5221 gtatatactt cgccacccca tttattggtt tccaccaatt cggttttatg aacaatttct
     5281 ctaagttttc ctagttcttc tgcccattgg tttactttat tccaagtatg tttttctgcc
     5341 atgattttgg aaaactaatt tttctttttt gttttttttg acttagaaaa atcagttgtg
     5401 gcagtgagtt cagcaatttt tacaattaca tctactgctt tttgcatact ttcaacagga
     5461 acgtattcgt attttccatg aaaattatga ccaccagcaa aaatattggg gcatggtaaa
     5521 cccatataag ataatcgaca accatcagtt ccgccacgaa taggtttgat aagtggtttg
     5581 attcccagtt ctttcattgc tttctcggcc aaatcaacaa tgaacattac aggaacaact
     5641 ttttctttca tgttgtaata ttgatctttg atttcgcaga ttacaatatc ttcaccaaat
     5701 tgtttggcaa aatgtttatt gattttctta gtgattttat gcaccaattc ttttcgtttg
     5761 atgaatttct tcctgtcatg atcacgaata atcatttcta gttttgtttc ttcaatactt
     5821 ccctgaatat ctgtaacatg aaaaaaacct tcgtagcctt tcgtttcttg aggtgtttca
     5881 ttttttggca attcgttgat aaaatcattg gcaatcaaca tcgaattaat catttttcct
     5941 ttggcataac ccggatgaac tgactttcct ttgaaagtga tttttactcc agcagcattg
     6001 aagttttcat attccaattc accaatttgg cttccatcca tggtataagc ccattcagct
     6061 ccaaattttt tcacgtcaaa taagtctgct ccacgaccaa tttcctcatc tggtgtgaaa
     6121 caaattctaa tttttccgtg tttgatttct ggattttgga ttagatattc cattgcagtt
     6181 acgatttcag taattcctgc tttatcgtca gcaccaagaa gtgttaaacc atttgtagta
     6241 attaacgttt gacctttgta ttgcaataaa tctttgaagt aacttggaga aagcacaatg
     6301 ttttcttttt tattcaaaat aatatcacca ccatcataat ttttgactat ttgtggtttt
     6361 acatcttttc cagtaaaatc gggcgtagta tcaaagtgtg aaacaaaacc aattgttgga
     6421 actttttcct ttatattcga tggaagtgtt gccatcacat agctatgttt gtcaatcgtt
     6481 acttctttca tgccaattgt ttttagttct tctactaatt tgttggcaag attccattgt
     6541 ttttctgtac ttggagtggt ttctgagttt ggatcagatt ccgtatcaat agttacataa
     6601 ctgatgaaac ggtctataat gtgttgcata atgtgttttt ttcaaagata caaattcaat
     6661 tttttgtata aaataattct ttacaaaggt tttgctttaa cttctttggt tgaaatagcc
     6721 gaaaccttgt cttcattgtt taatgtgaca tataattctc gtttatataa ttttttttga
     6781 tacgaaatca tatatctgaa aagatattct tcgagttctg gattataagt aatatcgatt
     6841 agttccaatc ccaaatattt gccatttcgc atgtttattt ttttgcaagt agcagcaatt
     6901 cgttcaggtg ttgcattatt gattacgttt tcggtggctt catcttttga aaaagcttta
     6961 aatttggaag tgttgcacgt ttctaaaagc cgatttccca attcaaaagc tcggtttttt
     7021 ttaaacgatt caacttcact tgcagtaaca gttcgaatgg cttttgaatc tttggtatca
     7081 cttactgttt tcttagattt acaagatgcg ataaatagta acaaactaat aaaaagtact
     7141 tttttcatga ttttggtttt taattaaacg atgaaaatcg gtttttagta taaaaaaatc
     7201 cagatttctc tggattttgt gttagtataa actcggaaaa tcacttggat ttacttcttg
     7261 catgatggca taaattttct caaaaatatc ttcaacagat ggtttagaaa aataatcgcc
     7321 atccgttcca tacgatggtc gatgtgcttg agctgccaaa gtttgtggtt gactgtctaa
     7381 atgtaaatag cctttttgaa cttcaagaat ctgttgtaaa atataagcgc ttgctccacc
     7441 aggaacatct tcatcaacca ctaaaagtct attggttttt tctaaacttt taacacaatc
     7501 atgatttata tcaaatggca ataaagattg tgcatcaata atttctatat cgataccaaa
     7561 atttaataac tctgcagcag cttgttctac aattctaagt gttgaaccat aagaaacaac
     7621 agtaatgtct gttccagttt taatagtttc aactacgcca ataggtgttt tgaaatctcc
     7681 taaattggta ggcatttttt cttttaatcg gtaaccattc aagcactcga ttactaaagc
     7741 tggttcgtca gtttgtaata aagtattata aaaaccagcc gctttggtca tgtttcttgg
     7801 cactaaaaca tgaataccac gaacagcgtt gataatcatt cccattggtg agcctgaatg
     7861 ccaaattcct tccaatcggt gtccacgtgt tctgatgatt aaaggtgcct tttgtcttcc
     7921 tgccgttcta tattgtaaag ttgctaaatc gtcactcata atttgaatgg catacaacaa
     7981 ataatctaag tattgaattt cggcaattgg acgcaatcct cgcattgcta atccaattcc
     8041 ttgtccaaga atggttgctt cacgaatacc aacatcggca acacgaagtt ctccatattt
     8101 ctcttgcatt ccttctaaac cttgatttac atcaccaata tttccagaat cttcaccaaa
     8161 aattagtgtt tcaggatatt ttgcaaaaat agcatcaaag ttatctctca ataccaaacg
     8221 cgcatccact tcttctgatg actcgctgta ggttggcagt acttcttttg cagaaagtac
     8281 atttttaact gattgtgaaa ataagtgtga actaaatttt ggttgtattt tttcagtata
     8341 atttttaatc caagttgcta aagtttgttg gccattttca ccagcaatta atcgcaatgt
     8401 ttttcgagcg gtaaccaaaa gttcttttcg aatgggttct tttatggctg ctaaatcagt
     8461 agcgtttttt tgaataaaaa cttgatttgg acttgtattg gctaatgaat ttaataaagc
     8521 tactaattca ttttgctctt ctttcatcgg agaaacaaag gcattccatg ctgccttttt
     8581 tccttctaaa actaaatttt ttgaaatttc atcaattgaa tccaattcaa tttcggtagc
     8641 aatattattt tttaataacc aattgcgcat ttgtgtcaaa caatcgaact cggtttccca
     8701 agccaatcgc tccgcatttt tataacgttc gtgcgaaccc gaagtagaat gtccttgtgg
     8761 ttgcgttaat tcttgaacgt gaattaaaac cggaacatgt tcttcgcgag caatagctcc
     8821 ggctttttga taggtttcta ctaatgttgg ataatcccag ccgtttactc taaaaatttc
     8881 gtagccttta ttattttcgt ctctttggaa acctttcaag atttcagaaa tattttcttt
     8941 agtggtttga tgacgagcgt gaaccgaaat tccgtattca tcatcccaaa cactcataac
     9001 cataggtact tgtagcactc cagcggcatt tatggtttca aaaaataacc cttcagaaga
     9061 agaagcattt ccaatggttc cccaagctac ttcgtttcct tgattggtga aatttgtaaa
     9121 actttctaaa ccggtaacat ttctatatat ttttgaagct tgtgccaatc ctagcaatcg
     9181 aggcatttga ccagcggtag gtgaaatatc agcactcgaa tttttttgtt gcattaagtt
     9241 tttccaattg ccattttcat ccagcgaatg tgtagcaaaa tgaccaccca tttgtcttcc
     9301 tgcactcatt ggatcaaaat tcaaatcggt atgaccatat aaaccagcga aaaactgttc
     9361 aatggttaat tgtccaatag ccatcataaa ggtttggtct cggtaatatc ctgaacggaa
     9421 atcgccattt tgaaacgctt ttgctaatgc taactgggga acttcttttc catcgccaaa
     9481 aattccaaac tttgcctttc cagtcaatac ttcttttctg cctaacaagc tacattcacg
     9541 gcttattcta gcagttttat aatcgttaag tacttcggct ttgaaatctt caaaagtcat
     9601 ttcagttttt aatagcgttt cagtcatagt tgaaaaaaat ttctaagaac aaatttagat
     9661 aaaaagtagt gaatatgggt aaagattgaa aaataatcga tgaagtaatg attatattcg
     9721 acttaaatac tttgaaaata ttgcagtata atcagtaaaa taattttttc cccactaatt
     9781 atttaattta aatagcgttt aaaaccattt tcttgtgaat aatcgaatca aagtatcagg
     9841 attaaaagtt ataataaaac gaattcgctc ctgataattt tgctgcccaa tttcccaacc
     9901 atttgatgaa tatacaggaa aatataattc aaaataatcg gttactaaat ttaatcgtac
     9961 gccgctatca tgaatgaatt taggtgacaa atttctgttt tttatcattc ccaaatctcc
    10021 ataaatttct atccaattcc aaatattggt acttgcattt attgttgtta tccattgatt
    10081 tgcaaatcga ttctctaatt tagatttaaa aaaaccatcc attggaataa tttgttgact
    10141 aaaaagacct ttggtttcag aacgtcccaa ataatcggat tcaaaaagat aatcagaagg
    10201 tctatctaca ccaaaatcaa agaagtttga agtagtggta ttgtataaaa aactaccggc
    10261 aaaaaaacgt aaattcactt gtcgattatt gttaaataaa tgtctgtatt gcatttcggc
    10321 agctagtttt ccaaatgttt tagacagttg aatatcgcca agaaaattaa aatgatgggt
    10381 aacttctgtt tttccattat aatatttagc attaaaaact tggtaacttt cggtgttttg
    10441 atcggttaaa aatgctgatt tttctcggtt gacaattatt tctttaattt gaaaagattg
    10501 tactctattg tctcgtaggt ttttaggtct aaattgaacc gaaattgttg ggtttagttt
    10561 ggtgtaataa gcatcaggtg catagtgaag ataatgacca ccaagtaaat acttgatatt
    10621 gttaatttta ctatctctat agtactgatt aatatatact actccttttc cagaaatatt
    10681 atttgttttt gttgagaatg ttggatttaa atcaaagatg aaaggtttgt ctaaaatggt
    10741 tttattatgt actcgaagac caggttggaa tccatcataa agattatact caagtgttgg
    10801 tacataaaga atttgattgt agtagggatc ttccaaatct ttcatgaaat taaacttgat
    10861 tggacgattg ttcagtcgaa aaccacgaag cgattgccaa ttgtttctca aattaaattc
    10921 gggtacttca ttttcatagt ttaatacaat tttgtcggca tcgtttcttg gaaaagtata
    10981 aacactatct tttgctatac tgtcaaccca atatttgaaa acaatttctt ttttcttaat
    11041 tccatatact ggaatgggaa cattggttgc agttttattt tttattgtta gacttacact
    11101 atcttttgtt ttagaaaaac tattgatttt gtagtctata atatctctag agttgatgat
    11161 tttatcaaaa aaccaatcga ttcttttagg tgaattcttc tttagaatgt tttcaaaatc
    11221 agtttctgta gtttgttgtt ttttgtttag ttcaaaaaac tgtttgatgc tttcttgaac
    11281 acgattttga cccaaataac tgtccaaata gtttagactt aaccctgcac ggtattttga
    11341 ggctattttt tcattaaatt taattaaaga atcttttggg ctacccaaag gttggtcaag
    11401 attttttctt gccatcagca tatagaaata gctgtattgt ccattgaaac ctaaactaat
    11461 tagattaaaa ccacgaagaa taccgagttt cgaaaggctt cccatcattt tactatctgg
    11521 gtgaaattcg tcaatgtatt tcatcatcaa atacacttga attccgtcat aaatccagtt
    11581 gtcttcgcgc tgattgagtt gtaaggtgtt gtgaatataa gtgttcaaat aggtttttaa
    11641 aaacttaagt tcatacataa actcatccgg aaacggacta atgaaattgg gcaattgatt
    11701 caatccataa aaagggtttc gctcataatc aacttgtgaa atcgttattt ttttatgtgg
    11761 ataatgacca agttgttcag caacatagtt tacgatctta tcaataacaa ttgcgcgttg
    11821 aatatcggtt aatcgactat ctcttaaatt ggaaacgaca tcaatatctt cgtttttaaa
    11881 acttaaaaat tctccttttt catcaagaaa taaactaaaa ttcaatctgt tttctccttt
    11941 gaaatggtat aacgtttggt tttcttttgc cgtttgattt atttcattca aatccgaagt
    12001 taatactaaa ttagaaggta ctttaatgga aagttcaaaa tcagataaag cattagattc
    12061 atcatctaaa ttaacgttgt cgtattttat aaaagtatta ttttcaaatc gcgatggcgt
    12121 taaaaaccaa ttctttatat tgaatgttcc tttttcgtca tatccaaatt ttgtaaatct
    12181 atcgcttgtt attttgacga tgtatgtcaa tttaaaaatc ttcttttcat ttggaagcaa
    12241 tttttctcga agacgaaatt gaatcacatc gggatgattg gcatcacgtt cccaagtgag
    12301 gaagtttgca ttttcatcaa gtattgtaat gtttgaagta atgccacgtt ctttttcgtt
    12361 tgctaaatga aaaccacgtt caaactcatc cgaaaaacgt ttggctaaag gcgagttttt
    12421 gttagaatac gcattgttcc aatcatttaa aaccacgtaa cttaaagtat cattactttg
    12481 attgaaatag gtcaactctt gtattactga aatcgttttt tgttccgaat caaattcagc
    12541 aatgagttta gaattgtgtt gcgcaatagt gtttaacgat aacaaaagca aactccaaaa
    12601 aacaagaagt tttaaagtta aaataacttt attatttgga gtatgcatta gtgtttttaa
    12661 ttaaaagtta gggcttaaat tgtatttttt atagaatcta tccaattctt caactacttc
    12721 ttctgcagta tcaacaatct taattaagtt catgtcttct ggactagcat tgtgttcttt
    12781 tccaatcatt acacttttaa tccaatcgat taaacccgac caaaattcgg aacctactaa
    12841 gataattgga aatttaccaa tctttttggt ttggattaat gttaccgctt caaacaattc
    12901 gtctagtgtt ccaaaacctc ctggcattac tacgaatcct tgtgcatatt tgacaaacat
    12961 cacttttcta acaaagaaat aatcaaagtt taagttttta tctttatcaa tatacggatt
    13021 gaaatgttgc tcaaaaggca attcaatatt caacccaact gaagttccgc caccgtgatg
    13081 ggcaccttta tttccagctt ccataattcc aggtccaccg ccagtgataa caccatagcc
    13141 tgctttacta atgttgaaag caattttttc tgctaacaga taatatttat tgtctggttt
    13201 tgttcttgct gaaccaaaaa tagacacaca cggaccaatt cgcgccatgc tttcatagcc
    13261 gtttacaaac tccgacataa ttttaaatat tgcccaggag tcattagttc taatttcgtt
    13321 ccagtctttt tgttttagtt tttcttgaag tatctcatct tcattttcaa aatcgttatt
    13381 tttcatcttc ttttatttta ttcttatttt tttaatcaaa cgtgctaatg taattctttt
    13441 ttcaaaaagg catctctata tttctttttt gattctttcc cattcccatt ttagcaagga
    13501 ataacattcc gaatcttcgt ttttcccatc aacaacataa tcttctcgca taattccttc
    13561 ttttataaat ccttggcgaa gtagtaatcg tagtgaagct tcattctctg ctgctatcaa
    13621 tgcctcaact ctgtggagtt tcaatgctgt aaaaccatat tccaaaatag cagtcaatgc
    13681 ttcggtcata tagccttgct gtttgttagc attattgtgg atgttataaa atatttcggc
    13741 gcgattgtgt ttggcagtcc atgtatgaaa accacattca ccaataggct tgttggtttc
    13801 tttatcaatc agcaaaaaga agaacaaaga aactgaatgc gtttccatgc ctttttcgtg
    13861 catcaattta tgatgttcat aacctatttc attaaaccca aagtagttaa tgatttcttc
    13921 tttcgattta gtgttgaatg cctcatgaat aaaagcagga gtaatgcttt tcaagagtaa
    13981 tcttgacgta gaaagagtta cttgatttga catatactat tactttaatt cctttttcaa
    14041 aaactgagcc gtatagcttt ttttgttttt aatgatctct tcgggtgttc ctttagcaat
    14101 gacttcacca ccttttttgc cgccttccat accaatatca atgatgtaat cggctagttt
    14161 tatcacgtcc atgttgtgtt cgatgattaa caccgaattt cctttgtcaa ccaaagcatt
    14221 gattacatcc atcaaaacac gaatgtcttc aaagtgcaaa cccgttgttg gttcgtccat
    14281 gatgtagaac gtatttccgg tgtctttttt agacaattca gtagctaatt tgatgcgttg
    14341 tgcttcgcca ccagagagcg tggtgctttg ttgtccaaga gtgatatatc ccaaacctac
    14401 atcttgaatc gtttttactt ttctatagat tttcggaatc atttcaaaga aatctaccgc
    14461 ttcatcaaca gtcatgttta gcacatccga aatggatttt cctttatatc taatttcaag
    14521 cgtttctctg ttaaaacgtt ttcccatgca ggtttcacat tccacataaa catcgggtaa
    14581 gaaactcatt tcaatcgttc gaacgcccga tccttcgcag gtttcgcatc gtccgccttt
    14641 tacattgaaa ctaaaacgtc ctgctttata gcctcgaatc atggcttcag gtgtcatggt
    14701 gtatagtttt ctgatttcgg aaaaaacatc tgtgtatgtt gctgggttac ttcgtggtgt
    14761 tcttccaatc gggctttggt caatgtcgat tactttatcg atgtgttcta aaccttccat
    14821 ttttttatac ggttgtggct tctttaccgc attaaagaaa tgctcgttca gaataggata
    14881 gagggtttca ttaatcaaag tcgattttcc acttcctgaa actccagtaa cacaaatgag
    14941 ctttcctaaa ggcaattcta tcgaaacgtt ttttaggtta ttaccagttg cgcctgttag
    15001 ttttaaaaat ttgccattgc cttcgcggcg tgtttttggc acttcaattt ccatcttgcc
    15061 attcatgaat tgcgccgtaa tcgtgttttc ctttagcaac tcttttggcg taccaatact
    15121 aatgatttcg ccaccatatt ttcctgcttt tggtccaatg tctattacat agtcggcacg
    15181 ttctatcatg tctttgtcgt gttctaccac aagaaccgag ttgccaatat ccctaaggtt
    15241 ttctaacgaa ttaatcaagc gttcgttgtc gcgttgatgc aaaccaatgc tgggttcgtc
    15301 taatatataa agcacaccaa ccaactgcga accaatttgc gttgccaatc ggatgcgttg
    15361 tgcttcacca cccgaaagcg attttgaact tctgcttaac gataaataat tcaagccaac
    15421 atccatcaaa aactgtaaac gggtgatgat ttcttttaaa acttcggtag caatggtttt
    15481 ttgtttttcg gataagtgtt tgtttaaatc ttcaaaccaa gctatcaatt ccgatatatc
    15541 catagccgat aaatcagcga tgtttttttc gtttatttta aagtaaagcg attctttttt
    15601 taggcgtgaa ccttcacaaa caggacaatt aatttcgtcc atgtattctt ttgcccaacg
    15661 ttttaaagaa gtcgaattcg cttcatcaaa ctggctttta atgaaatgcg aaatgccttc
    15721 aaagtcaatt ttataatctt tggtaacgcc tgctactttt gaggttaccg agaatttctc
    15781 tttgccaccg ttcataatca tgtccatcgc ttcggctgga attttctcga tggcatccgt
    15841 taatgtaaaa ccaaattttt cgccaatggt ttccaattgc ttgaacatcc atgaggattt
    15901 gtattcgccc aaaggagcaa aaccaccatt cttgatggat agttttgggt tgggaataat
    15961 tttcttttcg ttgatttggt gtaccgttcc taaaccgttg caattgtcgc atgcaccttt
    16021 gggtgagtta aacgaaaagt tattcggttc cggattcgga taggatattc ctgtggtagg
    16081 acacatcaag gttctgctga aaaagcgggt ttccttgctt tcatgttcaa taaccatcaa
    16141 gatgttttcg ccgtgataca tcgctgtgtt gatgctttcg gctaggcgtt ttttagtatc
    16201 gacgctatcg ttcacttcca gtctatcaat tacaatttcg atatcgtggg ttttataacg
    16261 gtcgagtttc atgcccgaaa cgatgtcttt aatttcacca tttacacgca ctttcaagaa
    16321 accttgttta gagatttgtt gaaacaattc ggcataatgt ccttttctgg ctctaatcac
    16381 aggagccaaa atattgatgc gttttccagt aaaatcttcg ataatcaaat ctctaatttg
    16441 ctcatcggaa taggaaacca ttttttcgcc agtgttataa ctatacgcat cggcggcacg
    16501 ggcaaataat aatcttagga aatcataaat ctcggtaatc gttccaacag tagaacgtgg
    16561 acttttactc gtagtctttt gttcaatagc aattactgga gaaagcccgt caattttatc
    16621 tacatcggga cgttctaatc cgccaagaaa ttgacgcgca taggccgaaa acgtctcgat
    16681 ataacggcgt tgtccttcgg cataaatggt gtcgaacgcc aacgacgatt ttcccgaacc
    16741 cgaaagacca gtaatgacta ctagcttttc tcggggaatg ttaacatcga tgttttttag
    16801 gttgtgcgca cgtgcgccaa tgatttcaat atattcttgg tcttccatag caattggcaa
    16861 aacgcaaaaa tacaattttc aacgcagatt ttaggtgcac actttagaag aatttgttaa
    16921 agatttttat atttggaaaa attaggaaag agaatagaaa atagagaaaa gatgatagat
    16981 tatatctttt aaacttataa acacataaac ttttaaacta taaaaaaaat gaaattacta
    17041 ggtataggtt cacgattaaa gcatgcagaa catggagtag gcgtggtgac gaacgttact
    17101 tcaaaacatt attgggttac gtttatcgaa aacggattgg aaaccataga catcgacagc
    17161 gactttgaag tgattgaagc attggaagac gaagtggatt cggtgagttt ttatgaagta
    17221 gagaaaactt tgaaaaacct gttgatgcgt tggagtgata tttcgccaaa cgttgctatt
    17281 gccgataaat ggaaaggcgg aaagctcatc atggaaccgg gcacaccaac ccaaaacaaa
    17341 gaagtgccta ttgatacgtt ctttcataaa atagtgatgg tgcgcgatag agtgcgcgtg
    17401 atggagcaaa aaataaactc cagcaacttg gaagaagccg acaagattga cctgcaacaa
    17461 tacattacac gtatctatgg aagtttgacg acttttaatg tgttgtttaa agcaccttct
    17521 gattattttg tggg
//
LOCUS       DAOOBH010000038        17358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_4270_length_17358_cov_5.675663, whole genome shotgun sequence.
ACCESSION   DAOOBH010000038 DAOOBH010000000
VERSION     DAOOBH010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 17358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17358
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4270_length_17358_cov_5.675663"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PK218_05710"
     CDS             complement(<1..388)
                     /locus_tag="PK218_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98037.1"
                     /translation="MSGFSEIEPTKLRLKNYLAKNKKPKYVIFNIDPFLKATIKKNKN
                     LINKNSYSVYAFLPSKKNLAIVNFYKFNNYEKYIPLYAIFKYKILFDCLTLNKSNSFP
                     NGFELNDEIWNQKKYPSSAINKKFFFK"
     gene            complement(632..2077)
                     /locus_tag="PK218_05715"
     CDS             complement(632..2077)
                     /locus_tag="PK218_05715"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPW98038.1"
                     /translation="MTFNSFEFLVFFLIFYFLYWSVFSGNLKIQNILLLISSYFFYAW
                     CNWKFLSILIISSILFYSLGNAISKTENEKKKTFFLHIAVFQSLGVLFLFKYLNFFIS
                     SVADFLAIFNLNADIHTLKIILPLGISFYTFRLLSYILDIYNEKIEPTTNWIIFFNYV
                     SFFPCLIAGPIDRADNLIPQLEKKREFSYQLAVDGFRQILWGLFKKIVVADSCAIITN
                     EIFDNYQDYPASSLLIGAFIYIIQIYADFSGYSDMAIGISKLLGFSIPKNFNYPFFAT
                     NIAEFWKRWHISLTSWMTEYVYTPLSFFFRKYGKYGIVISILINFILVGLWHGSQWTF
                     IVFGLLHGIYFIPLLLNGTLNKKNITKNGFKTFIDIITTFSIVMLTGVVFRSSSVSMA
                     CEFYINLFSSSFFSWPLITTGYMFLLVTVLYITIMFVIEWFGRQDSFGIATTFIKKSK
                     SIRWSFYIFLVLSIYYFSFSDKTQQFIYLQF"
     gene            complement(2087..2335)
                     /locus_tag="PK218_05720"
     CDS             complement(2087..2335)
                     /locus_tag="PK218_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPW98039.1"
                     /translation="MNRSEILEKIGIILTSVLGHDNFELQENLSAKDVDGWDSLTHMI
                     IITEIEAKFEVRFKLKELNKLNNMGNLLDLVSNKLAEK"
     gene            complement(2342..3004)
                     /locus_tag="PK218_05725"
     CDS             complement(2342..3004)
                     /locus_tag="PK218_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW98040.1"
                     /translation="MSSYILRQANNEDIPFLAKTIMEAEKSGTEKFSFSTLFNKNEKE
                     ALQLIEQMLEEEIVGCEISINSFKVIEFDGKVVAAFAGWIETFDTEITSGLLKSNLIG
                     YTFGKESIHFFMGKSNLLKEIQIKREPKTLQLEYLYVLPEYRGKKLTNELINKHINDA
                     RNLFLHLKKVQVQVFKNNSGAIKVYENCGFKITKVFKSKNEEILDYLPSNEKYLMEKK
                     LN"
     gene            complement(3004..4512)
                     /locus_tag="PK218_05730"
     CDS             complement(3004..4512)
                     /locus_tag="PK218_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW98041.1"
                     /translation="MNHQKEHDFFQSLKNNLIRHEKRNAFFINNVFYSYSELAQSISN
                     IRSIIKEKTNENEKIIGLITNDDLETYAAILALWFEGKAYVPLNPSVPNERNQKIINE
                     AGIITIIDSSELTVFANFNTIESKKIKTVSLNLNPTESADNNLVYLLFTSGTTGKPKG
                     VPISKENLEGFLSAFWHLNYDITEEDRFLQMFELTFDLSVMSFLIPLLSGSCIYLVPK
                     DKIKYSYIIELMEEQELTVALMVPSMLYYLRPYFDEIDLPKMKYSLFCGEALSLDITQ
                     EWSKCLPNSKITNVYGPTEDTIFCTFYDYKKLEPNKTYNGILSIGKSMLNNYSIIVDE
                     NNAILSAGKVGELCLGGVQLSSGYWKNEEKNQESFFYTNYNNKHERFYKTGDLCKIDE
                     FGDLLYLGRMDFQIKIQGYRVELSEIEYYAKEFLSKINVVVLTIIDHLGNNEVGMVIE
                     SKKFDSKELLEFLKLKLPYYMVPKKVNFIDNFPLNSNGKIDRKELETLFYNK"
     gene            complement(4602..6359)
                     /locus_tag="PK218_05735"
     CDS             complement(4602..6359)
                     /locus_tag="PK218_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW98042.1"
                     /translation="MEAKAFDSNLFKRILKYTKPYRARFYGVIAFAIFLSVFAALRPL
                     LLKHTVDDYLKPKDEHGLLFYVMLMAIVLLLEVFSQFYFVYWANWLGQDIIKDIRIKL
                     FKHMLSFRMKYFDNSPVGMLVTRSVSDIEQIARIFSQGLFMIISDLLKMIVILGFMFY
                     MNWRLTWIVILAMPVLVYATRKFQRKMQVAFEEVRTQVANMNTFVQERVTGMKIVQLF
                     SREEIEYQKFKAINEKHKKAWIKTILYNSIFFPIADFISSFTLGAVVIYGGFQILDGD
                     KFTTFGDLFSYTMFISMLFNPLRQIADKFNEMQMGMIAANRVFDIIDSTDTIQSNGIK
                     VAPHFEGIISFQNVRFGYIEEEEVLKGINLEVKSGETIAIVGSTGAGKSTIINLLNRF
                     YEINSGTITIDNQNIQEFELESLRKQIAIVLQDVFLFADTIYNNITLNNPEISREEVI
                     TAAKKIGIHDFLISLPNGYDYNVKERGVMLSSGQRQLIAFLRAYVSNPSILILDEATS
                     SIDTYSEELIQKATEKITQGRTSIVIAHRLATIINADKIVVMDKGEIVELGTHQELLL
                     KQNGYYKKLYDAQFSSEVI"
     gene            complement(6407..7141)
                     /gene="truA"
                     /locus_tag="PK218_05740"
     CDS             complement(6407..7141)
                     /gene="truA"
                     /locus_tag="PK218_05740"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPW98043.1"
                     /translation="MRHFIHLAYKGTNYHGWQSQPNAISVQEVLEKALQIILRKSIAI
                     VAAGRTDAGVHAKEMYAHFDFDEIINVEKLVKKLNSFLPKDIAIFKIIKVHDTAHARF
                     DAVKRTYQYHIHTQKNAFECNDSWYFAQNINIEKMNEACKILFAYTDFECFSKIHTDV
                     NTFDCKIYEAFWQQKENQLVFTIAADRFLRNMVRAIVGTLINIGLEKVSLEDFKKIIE
                     SKDRAKAGFSVPAHGLYLTKIEYAYL"
     gene            7261..7755
                     /locus_tag="PK218_05745"
     CDS             7261..7755
                     /locus_tag="PK218_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPW98044.1"
                     /translation="MIKILLLSDTHSHIDETILKYVRLADEIWHAGDIGNLQVTDEIK
                     KLKPLRAVYGNIDDDKARMEFPLNNRFFCENIDVWITHIGGYPGKYNQTIREEISKNP
                     PKLFICGHSHILKVQFDKKLNLLHMNPGAAGIHGFHQVRTMLRFEIEGEKIQNLEVIE
                     IGKK"
     gene            complement(7929..8336)
                     /locus_tag="PK218_05750"
     CDS             complement(7929..8336)
                     /locus_tag="PK218_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HPW98045.1"
                     /translation="MFRPQTYYLIGVLLINGILPFVFPLWKLDGKEVYFMSNVAYSSL
                     FGLSIVLSLLSILSHTKRQQQFVMGRLNIILNLILLGLFVYHTLNASGGTTAVPEKGV
                     GMFLPIFSIVLLVLANKAIKKDEDLVKSVDRLR"
     gene            8524..10203
                     /gene="rho"
                     /locus_tag="PK218_05755"
     CDS             8524..10203
                     /gene="rho"
                     /locus_tag="PK218_05755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPW98046.1"
                     /translation="MFDISALKEMKLAELQEIAKLAKTIKVTGVKKDKLIEDILAQQN
                     NATEATEKPKVIAKPKLEKPKRSRITADETSEKVEKQGELFANETPSENLTTTETKKP
                     EAKEKGSKFKKPVFEKKSSSIPPAKVEENAKEEVVADSSSQEKNQPRVPKHNHQNGNG
                     NSNPNEKANNNNPNYKGKKQNFRDADYEFDGIIESEGVLEMMPDGYGFLRSSDYNYLA
                     SPDDIYLSTSQIRLFGLKTGDTVKGVVRPPKEGEKFFPLVRVLKINGHDPQVVRDRVS
                     FEHLTPIFPKEKFNIAEKHTSISTRIIDLFSPIGKGQRGMIVAQPKTGKTMLLKDIAN
                     AIAANHPEVYLIVLLIDERPEEVTDMQRSVRGEVIASTFDREPMEHVKIANIVLEKSK
                     RLVECGHDVVILLDSITRLARAYNTVQPASGKVLSGGVDANALQKPKRFFGAARNVEN
                     GGSLSIIATALTETGSKMDEVIFEEFKGTGNMELQLDRKIANRRIFPAIDLTSSSTRR
                     DDLLLDENTLKRMWILRKFLADMNPVEAMDTINDKIKKTRNNQEFLISMND"
     gene            10246..10617
                     /locus_tag="PK218_05760"
     CDS             10246..10617
                     /locus_tag="PK218_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HPW98047.1"
                     /translation="MFKFNQNYFIAFSLLLFTEIFIALYVNDSFIRPYFGDYLVVILI
                     YCFVMSILVFSKWKTAFYVLFFAFVVEFLQYLDCIKELGLQNNKFAKIIIGTSFSWHD
                     VLCYILGYFTIVGFEILKKSK"
     gene            10821..12704
                     /gene="dnaK"
                     /locus_tag="PK218_05765"
     CDS             10821..12704
                     /gene="dnaK"
                     /locus_tag="PK218_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPW98048.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGGEPVVIANAEGKRTTPSVIAFVD
                     GGEIKVGDPAKRQAVTNPTKTVASIKRFMGNKYSESAKEASTVAYKVVKGDNDTPRVD
                     IDGRLYSPQELSAMTLQKMKKTAEDYLGQTVTEAVITVPAYFNDAQRQATKEAGEIAG
                     LKVMRIINEPTAAALAYGLDKKGKDQKIAVYDLGGGTFDISVLELGDGVFEVLSTNGD
                     THLGGDDFDQVIIDWMANEFNSEEGIDLRKDPMALQRLKEAAEKAKIELSSSTQTEIN
                     LPYVTATASGPKHLVKTLTRAKFEQLAEILVKRSMEPVAKALKDAGLSTSDIDEVILV
                     GGSTRMPVIQEQVEKFFGKKPSKGVNPDEVVAIGAAIQGGVLTGDVKDVLLLDVTPLS
                     LGIETMGNVMTKLIESNTTIPTKKSQVFSTAADNQPSVEIHVIQGERTMAADNKTIGR
                     FHLDGIPPAPRGVPQIEVTFDIDANGIIKVSATDKGTGKSHDIRIEASSGLTPEEIER
                     MKKDAEMNAENDRVAREKAEKMNEADAMIFQTESQLKELGEKLSDDHKVGIESALTEL
                     RMAHQNQDLEAIQKGLDNVNAAWKVATEAMYAQGEGQSQEAQPQADATGDQTQDVDFE
                     EVK"
     gene            complement(12827..13312)
                     /locus_tag="PK218_05770"
     CDS             complement(12827..13312)
                     /locus_tag="PK218_05770"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HPW98049.1"
                     /translation="MKNRLVKLALLFLIALMISCNQNSKKKFEKLQQISWLEGNWENK
                     LDDGLLIEKWKKNNDSVYLGKSYFIRGNDTLHFESIELIQRNDDLLYIPTVKGQNNNE
                     PIEFKLSKSTENTFTFENSSHDYPQTIEYKKVTDSQLSATVSGNQDGKVSSDVYLLNK
                     K"
     gene            complement(13397..14671)
                     /locus_tag="PK218_05775"
     CDS             complement(13397..14671)
                     /locus_tag="PK218_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPW98050.1"
                     /translation="MTFTTIRKYFNIILFQKLIVAAILISILSSFLAISLKHITEYLE
                     LSFLLKATKQNFLMFVFPIIGFFVIHFLRHYLFKKKENKGIKEIFDATNSKNKSLPLY
                     KVPSHFINGLFTVAFGGSTGIEVSTVVATAAIGAATHKKENLFKNYKTELICAGITAG
                     ITILFSSPIAGILFSMEVIAKKKNKIFFITNSIALAVAYGLLLILDEEPLFSVIIKSW
                     NYYAIPYFILLGILAGFNSVLLTKMVLFFKKQFSKIENDFHKILLGSFIISVLLFILP
                     QLYGDGYHAMKALFISGKTAFSISFIVTFIGILILKPIITSATLASGGDGGVFAPSLF
                     IGAFLGFFVATVLNTYFNLNVIPINFMVLGMAAVLSASLHAPYTSLFLVCGMINDYTL
                     FLPILMVCFIAKYTSMFLYPFTVYTYSPVVTN"
     gene            14821..15894
                     /gene="prfA"
                     /locus_tag="PK218_05780"
     CDS             14821..15894
                     /gene="prfA"
                     /locus_tag="PK218_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPW98051.1"
                     /translation="MLERLQYVKQRFDEVSDLIIQPDVIADQKRYVQLNQEYKGLKDL
                     MEKREELMLITANIEEAKEIIADGSDAEMTEMAKMQLDEAQDRLPELEDEIKFMLIPK
                     DAEDAKNVMVEIRAGTGGDEASIFAGDLFRMYTKYCETRGWRTSVVDMNEGTSGGFKE
                     IIFEVTGEDVYGTLKFEAGVHRVQRVPQTETQGRVHTSAATVMVLPEAEEFDVQIDMN
                     DVRVDFFCSSGPGGQSVNTTKSAVRLTHIPTGLVAQCQDQKSQHKNKDKALEVLRSRL
                     YEQELAKKQAEDAVKRTSQVSSGDRSAKIRTYNYSQGRVTDHRIGLDVFDMDGVMNGK
                     IQKFVDELQLVNNMEKLKESEVF"
     gene            complement(16006..17199)
                     /locus_tag="PK218_05785"
     CDS             complement(16006..17199)
                     /locus_tag="PK218_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98052.1"
                     /translation="MKTNIITLTMLSLMSLNFAFAQDRTTVTANNSEISDNLDLRAVA
                     SIFGDSKDLDDFEYRLNNPEYQISNLDLNNDNKVDYLRVIETVEGNVHLIIIQSVLGR
                     DLFQDIATVEVTKDRDSRVQVQVVGDVYMYGTNYIYEPVYAYTPVIYTTFWAPRYRPY
                     YSSWYWGYYPSYYVAWHPFPIFRYRNNINVWINFNHHYNYVNTRNCYAVYNNYYRGRR
                     GNAYEVQHPNRSFTNRHSGYVNRYELDKTRNIRTVGTVGTRDEIASNNIRNSKNDFNS
                     TRATDNSSSVRSNNTRNTGTVRENTTTGGVRSSNSSVSVKQETPVRNTTVREIAPTRN
                     NGSIGSSTNESIRTQPTRSSNSNSSVRSHSSTSRNLSSSRSVSSGSSSRSSTSGSSSR
                     GNSRR"
BASE COUNT         6200 a         2634 c         2674 g         5850 t
ORIGIN      
        1 gtttaaaaaa aaatttttta ttaattgctg agctaggata ttttttttga ttccaaattt
       61 catcatttaa ttcaaatccg tttggaaatg aatttgactt atttaaagta aggcaatcaa
      121 ataaaatttt atacttgaat atagcataca gcggaatata tttttcatag ttattgaatt
      181 tataaaaatt tacaatagca agattttttt tgcttggtaa aaaggcataa actgaataac
      241 tgtttttatt aattaaattt ttattttttt taatcgtagc ttttaagaaa ggatcaatat
      301 taaatataac atattttggt tttttatttt tagctaaata atttttcaat ctcagttttg
      361 tgggttcaat ttctgaaaaa ccactcattc caatatttac agattttaag ttgtaatttt
      421 cttcaaaaaa attaggatca aaatgtgccc aacaacgtga acttccaata aaaactaaat
      481 cagcattaat gttttcagtg gtgtcaagag tgtcaaaacc tgtaacactt tttcccttta
      541 tccttgttga tattgctatt tgaatgacta aaataaccgt aaaaaataga caaataaata
      601 gaactacgtt tttaaataga tgtttcattt tttaaaactg taaataaatg aattgttgtg
      661 ttttatcact aaacgaaaaa taataaattg aaaggactaa aaaaatataa aaactccatc
      721 taattgattt tgatttttta ataaaagtag ttgcaatgcc aaatgaatct tgtcttccaa
      781 accattctat aacgaacatt atagttatat aaagaactgt taccaataaa aacatatagc
      841 cagttgtaat taatggccaa ctaaaaaatg aacttgaaaa aagatttata taaaattcgc
      901 aagccataga aacactgctt gatctaaata caacacccgt taacattacg atgctaaaag
      961 tggttatgat atctatgaaa gttttgaaac cattttttgt tatatttttt ttattcaaag
     1021 taccatttaa aagcaatggt ataaaataaa taccatgcag taaaccaaac actataaaag
     1081 tccattggct tccgtgccac aacccaacaa gaataaaatt gattagaatt gaaattacta
     1141 taccatattt accatatttt ctaaaaaaaa acgataaagg cgtataaaca tactctgtca
     1201 tccaactggt taacgaaata tgccaacgct tccaaaattc tgcaatattg gtagcaaaaa
     1261 aaggatagtt gaaatttttt ggaatagaaa accctaataa cttagatatt cctattgcca
     1321 tatcagaata gcctgaaaaa tcagcatata tttgaataat ataaataaag gcaccaatca
     1381 ataaagaact tgcaggataa tcttgatagt tatcaaaaat ttcatttgtt attattgcac
     1441 aactatctgc aacaactatt tttttaaata atccccaaag tatttgacga aacccatcta
     1501 cggctagttg atagctgaat tctcgttttt tttcaagttg aggaatcaaa ttatcggctc
     1561 tatctattgg accagcaatt agacaaggaa agaatgaaac ataattgaaa aaaataatcc
     1621 aattggtggt tggttcaatt ttttcgttat aaatatctaa tatataactt agtaatctaa
     1681 aagtataaaa actgattcct agcggtaata ttatttttaa agtatgaata tcagcattta
     1741 gattaaagat agccaagaaa tcagcaactg aactaatgaa gaagtttaaa tatttaaaca
     1801 aaaaaagcac tcctaatgac tgaaaaacag ctatgtgtaa aaaaaaagtt tttttctttt
     1861 cgttttcagt tttagaaatt gcatttccta aggaataaaa tagtattgaa ctaataatta
     1921 aaattgataa aaatttccaa ttacaccatg cataaaaaaa ataactgctt attaataaca
     1981 gtatattttg aatttttaaa ttgccagaaa aaacactcca atataaaaag taaaaaatta
     2041 aaaaaaacac caaaaactca aatgaattga aagtcatgtt cgagatttat ttttcggcta
     2101 atttattgct tactaggtct aaaagatttc ccatattatt aagtttgttt aattctttta
     2161 atttaaatct aacttcaaat tttgcttcaa tttcagtaat aataatcatg tgagttaaag
     2221 aatcccaacc atctacatct tttgctgata aattttcttg taactcaaaa ttatcatgtc
     2281 ctaataccga agtcaatata atgccaattt tttctaaaat ttcacttcta ttcataattg
     2341 tttagtttaa ttttttttcc attaaatatt tttcgtttga cggcaaatag tctaatattt
     2401 cctcattttt tgatttaaaa actttagtaa ttttaaaacc acagttttca tatactttga
     2461 tagcaccgct attattttta aaaacctgaa cttgcacttt ttttaggtgt aaaaataaat
     2521 ttcttgcatc attaatatgt ttattaatta actcatttgt caattttttt cctctgtatt
     2581 cgggtaaaac atataaatat tctagttgta atgtttttgg ctctctttta atttgtattt
     2641 cttttagtaa attggatttg cccataaaaa aatgaatgct ttctttgcca aaggtataac
     2701 caattaaatt tgattttagt aaaccagaag taatttctgt atcaaaagtt tctatccaac
     2761 ctgcaaatgc tgctactact tttccgtcaa attcaataac cttaaaacta tttattgaaa
     2821 tttcacaacc aactatttct tcttctagca tttgttctat tagttgcaat gcttcttttt
     2881 catttttatt aaaaagagtt gaaaaactaa acttttctgt tccacttttt tcagcttcca
     2941 tgatggtttt tgctaaaaaa ggaatgtctt cattgttagc ttgtcttagt atatagctac
     3001 tcattattta ttataaaaca gtgtttcaag ttcttttctg tcgattttgc cattgctatt
     3061 aagtggaaag ttatcaataa aattaacttt tttaggaacc atataatatg gaagtttaag
     3121 tttcaagaat tcaagtaatt ctttactatc aaattttttt gattcaatta ccattcctac
     3181 ttcattattt cctaaatgat caattattgt taatacgaca acattaattt ttgataaaaa
     3241 ttctttagca taatattcta tttccgacag ttctacacga taaccttgaa ttttgatttg
     3301 aaaatccatt cttccaaggt acaataaatc tccaaattca tctattttgc acaagtcacc
     3361 agttttgtaa aaccgttcat gcttgttgtt gtaattggta taaaaaaaac tttcttgatt
     3421 tttttcttca tttttccaat aacctgatga taattgaact ccacctaaac aaagctctcc
     3481 aacctttcca gctgataaaa tagcattgtt ctcatctaca attatcgaat agttattcag
     3541 cattgatttt cctattgaaa gaattccatt atacgtttta tttggttcta attttttata
     3601 atcataaaaa gtacaaaaaa tggtgtcttc tgttggtcca taaacatttg ttatttttga
     3661 atttggcaaa catttactcc attcttgagt tatgtctaac gaaagtgctt ctccacaaaa
     3721 taaactatac ttcattttgg gtaaatctat ttcgtcaaaa taaggtctca aataatacaa
     3781 cattgacgga accattaacg ctacggtcaa ttcttgttct tccattaatt caatgatata
     3841 gctgtatttt attttgtctt tgggtactaa atatatgcat gaaccgctga gtaatggaat
     3901 taaaaaggac attaccgata aatcaaaagt taattcaaac atttgaagaa atcgatcttc
     3961 ttctgtaata tcatagttta gatgccaaaa cgcacttaaa aagccttcaa gattttcttt
     4021 ggaaataggc acaccttttg gttttcctgt agttcctgaa gtaaatagta aataaactag
     4081 gttgttatca gctgactcag ttggatttaa attgagtgaa actgttttta ttttcttgct
     4141 ttctattgta ttaaaatttg caaaaacagt aagctctgaa gaatctataa ttgtgataat
     4201 tccagcttca ttaatgattt tttgatttct ttcatttgga acactgggat ttaaaggcac
     4261 ataggctttt ccttcaaacc acaaagcaag aatggcggca taagtttcta aatcatcgtt
     4321 ggtaataaga ccaattattt tttcattttc attagttttt tcttttataa tacttctaat
     4381 gtttgaaatg gattgtgcta attctgaata agagtaaaaa acattattta taaaaaaagc
     4441 atttcgtttt tcatgtctaa tcaagttgtt ttttaaagac tgaaaaaaat catgttcttt
     4501 ttgatgattc atttattagt gcaattttta tagtttaaga ttcacaaaaa taaaaattat
     4561 aacctaatag caatcaataa tattcaaatt gtgtgaaata tttaaattac ttcactcgaa
     4621 aactgtgcat catataattt tttgtagtat ccgttctgtt ttaatagtaa ttcttgatgc
     4681 gttcctaact ctacaatttc tcctttgtcc atcactacaa ttttgtctgc atttattatt
     4741 gttgccaatc gatgggctat aacgatggaa gttctgcctt gtgtaatttt ttcggtcgct
     4801 ttttgaatca attcttcact ataagtgtca atagaagatg ttgcttcatc taaaattaaa
     4861 atactcggat tactaacata tgcccgaaga aaagcaatta attgcctttg cccagaagaa
     4921 agcataacgc cacgttcttt gacattataa tcataaccat ttggaagaga aattaaaaaa
     4981 tcatgaatac ctattttttt tgcagcagta attacttctt cacgacttat ttctggattg
     5041 tttaaagtaa tattgttata aatagtatca gcaaaaagaa aaacatcttg aagtacaata
     5101 gcaatttgtt tccgcaaact ctctaattca aattcttgta tattttgatt gtcaatagta
     5161 atcgttcctg aatttatttc ataaaaacgg tttagtaaat tgataatcgt agattttcca
     5221 gcgccagttg aacccacaat tgcaatcgtt tcacccgatt taacctctaa atttattcct
     5281 ttcaaaactt cttcttcctc aatataaccg aatcgaacat tttgaaaact gataattcct
     5341 tcaaaatgtg gtgcaacttt gattccattg ctttgaatag tatcagtact atcaataata
     5401 tcaaacacac gattggcagc aatcatgccc atttgcattt cattaaattt atcagcaatt
     5461 tgccgcagag gattaaacaa catgcttata aacatagtgt aagaaaataa atcgccaaaa
     5521 gtggtaaatt tatcaccatc taaaatttga aaaccgccat atataacgac agctccaagg
     5581 gtaaacgagg atataaaatc agcaatagga aaaaagatag agttgtataa aatggttttt
     5641 atccaagctt ttttgtgctt ttcatttata gctttaaatt tttgatattc aatttcttct
     5701 cgactaaaca attgaacaat tttcattcca gtaactcgtt cttgcacaaa agtattcatg
     5761 tttgccactt gagttcttac ttcttcaaaa gcgacttgca tttttcgttg aaattttcga
     5821 gtagcatata ctaaaacagg catggctaaa attacaatcc aagtcaaacg ccaattcata
     5881 tagaacataa agccaagaat aacaatcatt tttagcaaat cactgataat cataaataat
     5941 ccttgactaa aaatacgtgc aatttgttca atgtcagaaa cagaacgggt aacaagcatt
     6001 ccaaccggtg agttatcaaa atatttcatt ctgaaactta gcatgtgttt gaacaatttt
     6061 atacgaatat ctttgatgat gtcttgccca agccagtttg cccaataaac aaaatagaac
     6121 tgtgaaaaaa cttctaacaa taagactatt gccattaaca ttacataaaa taacaatccg
     6181 tgttcatctt ttggtttgag ataatcatca acagtatgct tcaataaaag cggtcgaagt
     6241 gctgcaaaaa cagataaaaa aatggcaaat gcaatcacac cataaaacct tgctctgtaa
     6301 ggtttggtat attttaaaat tcgtttaaac aaatttgaat caaacgcttt tgcttccatc
     6361 aattttataa aatattaaat tccaaattct atcttccaag tttcatttac aaatacgcat
     6421 actcaatttt tgtcaaatac aaaccatgag ctggaactga aaacccagct tttgctcgat
     6481 ctttactttc aatgattttt ttgaaatctt ctaaagatac tttttccaaa ccaatattta
     6541 tcaatgttcc tacaattgca cgaaccatat tcctaagaaa ccggtcggcc gctatggtaa
     6601 aaaccaattg attttctttt tgttgccaaa aagcttcata gattttacaa tcaaaagtat
     6661 ttacatcagt atgtatttta gaaaaacatt caaaatcggt gtatgcgaaa agtattttac
     6721 aagcttcatt cattttttca atattgatat tttgagcgaa ataccaacta tcattacatt
     6781 caaatgcatt tttttgagta tgaatgtggt attggtatgt tctcttgact gcatcaaatc
     6841 gggcatgtgc agtatcatga actttgatga ttttaaaaat agcaatgtct tttggaagaa
     6901 aagaatttag tttttttact aatttttcta cattaatgat ttcatcaaaa tcaaaatgag
     6961 catacatttc tttggcatga acaccagcat ctgttctacc agcagctaca attgcaattg
     7021 attttcggag tataatttgc aatgctttct ctaaaacttc ttgaaccgaa atggcgtttg
     7081 gttgcgattg ccaaccatga taattggttc ctttgtaggc taagtgaatg aaatgtctca
     7141 aaagtataat tttcgggttt cgtgttgtgg atttcgagtt tgtatctcgc aaatttacaa
     7201 agattttatg ttttaatacc ttaaaagttt gtagcccatt ttttttatat ttttgtattt
     7261 atgataaaaa ttcttttact ttccgataca cacagtcata ttgacgaaac cattttaaaa
     7321 tatgttcgtt tggcagatga aatttggcat gctggagata ttggaaactt acaagttaca
     7381 gatgaaatta aaaaattaaa gccacttcga gccgtttatg gtaacattga cgatgacaaa
     7441 gcaaggatgg agtttccgtt gaacaatcgt tttttttgcg aaaatatcga tgtttggatt
     7501 acacatattg gtggttatcc tggaaaatac aatcaaacga ttagagaaga aataagtaaa
     7561 aatcctccta aattatttat ttgtggtcat tcacatatac ttaaagtgca gttcgataaa
     7621 aaattaaatt tattacacat gaatcctggt gcagccggaa ttcatggctt ccatcaagtg
     7681 cgaacgatgt tgcgatttga aattgaaggc gaaaaaatcc agaacttaga agtaattgaa
     7741 atagggaaga agtagtggtc ggttggtggt ggttaatgtt ctgtttgtaa tttcaaaatc
     7801 aaaaatcaat aattaatata cctctatatt gggtttttat tgtttttaaa gctgggaagc
     7861 aatttcaaat aaaaaaccca aacgaaagtt tgggtttttt gcagcactaa aaaactaagt
     7921 ttataggttt atcgtaatct atctacagat tttacgagat cttcatcctt tttaatggct
     7981 ttgtttgcca aaaccaaaag tacgatagaa aaaataggta agaacatccc aacacctttc
     8041 tctggaacag cagtcgttcc tccagatgca tttagtgtat gatatacaaa taatcctaat
     8101 aaaattaaat tcaatattat gttcaatctg cccatgacaa attgctgttg tctttttgta
     8161 tgcgataaaa tgcttagtag tgatagaact atacttaacc caaataatga cgaataagca
     8221 acatttgaca taaaataaac ttcttttcca tcaagtttcc acagtggaaa aacaaagggc
     8281 aagatgccat ttattagtaa tacaccgatt aagtaatagg tttgtggtct aaacattttc
     8341 aaaatttgaa gtacaaaaat aaagtttctt ttttaataaa tactattgca acctaaaatt
     8401 ttatttgtat tattgcatgg caaaaccgta agtacttcat cctacggacg attaaaagat
     8461 acatttttct acttattttt tttatttcct ttttaaacta atttaatacc acaaaggaca
     8521 ttcatgtttg atatttctgc cttaaaagaa atgaagcttg ctgagcttca agaaattgcc
     8581 aaattggcaa aaactataaa agttaccggt gtaaaaaaag ataaactcat tgaggatatt
     8641 ttagcacaac aaaataacgc aaccgaagca actgaaaagc ccaaagttat agctaaacct
     8701 aagttggaaa agccaaaacg cagtagaatt actgccgatg aaacttctga aaaagtagaa
     8761 aaacaaggtg aattgtttgc aaatgaaact cctagtgaga atcttacaac tacagaaact
     8821 aagaaacctg aagcaaaaga aaaaggttct aaatttaaaa aaccagtttt tgaaaaaaaa
     8881 tccagttcaa ttcctcctgc aaaggttgaa gaaaacgcaa aagaagaagt tgttgctgat
     8941 tcttcaagtc aagaaaaaaa tcaacctaga gttccaaaac acaatcatca aaatggtaat
     9001 ggaaattcaa atcctaacga aaaagccaat aacaacaatc caaattataa gggaaagaaa
     9061 caaaattttc gcgatgcaga ttatgagttt gacggaatca tagaaagcga aggcgttctt
     9121 gaaatgatgc ctgatggtta tgggttttta cgttcttcag attataatta tttggcatcg
     9181 ccagacgata tttatttgtc aacttcccaa attcgtttgt ttggtttgaa aactggtgat
     9241 actgtaaaag gtgtagttcg accgccaaaa gaaggagaga aattttttcc tttagttcgc
     9301 gttttaaaaa ttaacgggca tgatccacaa gtagttaggg atagagtttc ttttgaacat
     9361 ctaactccta tatttccaaa agagaaattt aatattgccg aaaaacatac aagtatttct
     9421 acacgaatta tcgatttgtt ttctccaatt ggaaaaggac aacgtggaat gattgtggcc
     9481 caaccaaaaa cgggtaagac aatgctttta aaagacattg caaatgccat tgctgccaat
     9541 catcccgaag tttacttaat tgttttatta atagatgaac gtccggaaga agtaactgat
     9601 atgcaacgaa gtgttcgtgg tgaagtaatt gcatctactt tcgatagaga accaatggag
     9661 catgttaaaa ttgccaatat agttctcgaa aaatcaaaac gattggtaga atgcggtcat
     9721 gatgttgtaa tcttattaga ttcaattacg cgattagcta gagcttataa cacagtacaa
     9781 cccgcatctg gaaaagtact ttcgggtgga gtagatgcca atgctttgca gaaaccaaaa
     9841 cgtttctttg gtgcagctcg taatgtagaa aacggtggtt cattaagtat aattgctacg
     9901 gctttaacag aaactggatc aaaaatggac gaagtaatct ttgaagaatt taaaggaaca
     9961 ggaaatatgg aactgcaatt ggatagaaaa attgccaacc gtagaatttt ccctgctatt
    10021 gatttaacgt cttcaagtac gcgtcgagat gatttattat tagacgaaaa tacattaaaa
    10081 agaatgtgga tacttagaaa attccttgca gacatgaatc cagtggaagc aatggatact
    10141 attaatgaca agattaagaa aaccagaaac aatcaagaat tccttatttc gatgaatgac
    10201 taaaataatt acaatcccaa ttttttggga ttttttttta agacaatgtt caaattcaac
    10261 caaaattatt ttatagcttt ttctttgctt ttattcacag agattttcat tgcattatat
    10321 gtcaacgata gtttcatccg accgtatttt ggcgattatc tcgtggtgat tttaatctat
    10381 tgttttgtaa tgagtatttt ggtgttttcg aaatggaaaa cagcttttta tgtattgttt
    10441 tttgcttttg tcgtagagtt tttgcaatat ttagattgta tcaaagaatt aggtttacaa
    10501 aataataaat ttgctaaaat aattattggc acctcttttt catggcatga tgtactttgt
    10561 tatattttgg gatattttac cattgttgga tttgaaattt tgaaaaaatc aaaataaaat
    10621 ctgaaacctt gaacttctac tcatgtactt cttgcttcgt atattagatg tcatcttgtc
    10681 atacttagtc ctttcaaaac tgacaattta ttcacattcc ttccttggca taaatgttga
    10741 ctaaatgtaa gtgtgcaaca caacgcacta atcactttaa gtgaaataat aaaatttaaa
    10801 attaaaaaga agaaaataaa atgggaaaaa ttattggtat cgatttaggt acaaccaatt
    10861 cttgtgtttc agttatggaa ggtggagaac cagtagtaat tgctaatgca gaagggaaaa
    10921 gaacaacacc gtcagtaata gcatttgttg atggtggcga aattaaagta ggagatccag
    10981 caaaaagaca agcagtaact aatcctacta agactgtagc atccataaag agatttatgg
    11041 gtaataaata ttcagaaagt gccaaagaag catcgactgt agcatataaa gtggtaaaag
    11101 gtgacaatga tactccaaga gttgatattg atggaagact ttattcgcca caagaattgt
    11161 cagcaatgac acttcaaaaa atgaaaaaaa ctgccgaaga ttatttagga caaaccgtta
    11221 cagaagctgt tattactgtt cctgcttatt ttaatgatgc acaacgtcaa gctacaaaag
    11281 aagctggaga aattgcagga ttaaaagtaa tgcgtatcat caatgagcca actgctgcgg
    11341 cattagctta tggattggac aaaaaaggaa aagatcaaaa aatagcagtt tacgatttag
    11401 gtggtggtac ttttgatatt tcagtattgg aattgggcga tggtgttttt gaagtattgt
    11461 caactaatgg agatactcac ttaggaggtg acgattttga ccaagtgatt atcgattgga
    11521 tggcaaacga atttaactca gaagaaggaa tagatttgcg aaaagatcct atggcgttac
    11581 aacgtttgaa agaagctgca gaaaaagcaa agattgaatt atcatcttct acacaaaccg
    11641 aaattaactt gccatatgtt accgctacag cttcaggacc taagcactta gttaaaactt
    11701 taactcgtgc taaatttgaa caattagcag aaatattagt aaaacgttca atggaaccag
    11761 ttgctaaagc gttgaaagat gcaggtttat caacttcaga tattgatgaa gttatcttag
    11821 ttggaggttc tacacgaatg ccagttattc aagaacaagt agaaaaattc tttggaaaaa
    11881 aaccatcaaa aggagtaaat cctgatgaag ttgtagctat tggagcagca attcaaggtg
    11941 gcgttttaac aggtgatgta aaagatgtat tgttattaga tgttacacct ttatcattag
    12001 gaattgaaac tatgggtaat gtgatgacga aattaattga gtcgaatact actattccaa
    12061 ctaaaaaatc acaagtattc tctacggcag cggacaatca accaagtgtt gaaatacacg
    12121 taattcaagg agaaagaaca atggctgctg ataataaaac aattggtcgt ttccacttag
    12181 atggaattcc accagcacca agaggtgttc ctcaaattga agtaactttt gacattgatg
    12241 ctaatggtat catcaaagtt tcagcaactg ataaaggaac tggaaaatca catgacattc
    12301 gtatcgaagc ttcctctggt ttaacaccag aagaaattga aagaatgaaa aaagatgctg
    12361 aaatgaacgc agaaaacgat agagttgcaa gagaaaaagc agaaaaaatg aacgaagccg
    12421 atgctatgat tttccaaaca gaaagtcaac taaaggagtt gggtgagaaa ttatctgatg
    12481 atcataaagt aggcattgaa tcggcattaa cagaattgag aatggcacat caaaatcaag
    12541 atttagaggc aattcaaaaa ggtcttgata atgtaaatgc tgcttggaaa gttgctacag
    12601 aagcaatgta cgcacaaggc gaaggtcaat ctcaagaagc gcaaccacaa gctgatgcta
    12661 ctggcgatca aacacaagat gttgattttg aagaggtgaa ataatttttg aattaagggt
    12721 tactatattt aaaccgagtt agaaataact cggtttttta tttgaataac cataacgttt
    12781 aaacaaaaaa atgctcacaa aatttgtgag cactagataa tatatattat tttttgttta
    12841 aaagatatac atcagaactt acttttccat cttgattacc ggaaactgtt gcacttaatt
    12901 gtgaatcggt tactttttta tactcaatag tttgagggta atcgtgagaa ctattttcaa
    12961 aagtgaatgt gttttctgta cttttagata gtttaaactc tataggttca ttattatttt
    13021 gacctttaac agttggaata taaagtaagt catcatttct ttggattaat tcaatacttt
    13081 caaaatgaag tgtatcattt ccacgaataa aatagctttt ccctaagtaa acgctatcat
    13141 tatttttttt ccatttttca attaaaagac catcgtcaag tttgttttcc cagtttcctt
    13201 caagccaaga gatttgttga agtttttcaa atttcttttt tgaattttga ttgcacgaaa
    13261 tcattaaagc aatgaggaat agtaatgcta atttaaccag tctatttttc atagaaaaaa
    13321 aattaattgg ttgtaaaatt agtaaactta tttttgtttt tacttttttt taaaaataaa
    13381 aacgacacct taaaaactaa ttagttacga ctggagaata ggtgtaaacg gtaaagggat
    13441 acagaaacat tgaagtatat tttgcaataa aacaaaccat aagtatgggt aaaaacaaag
    13501 tataatcatt tatcattccg caaactaaaa ataaggaagt gtatggtgca tgaagactag
    13561 cactcaatac tgcggccatt ccaagtacca taaaatttat aggaattaca ttaagattaa
    13621 aatatgtatt caaaactgtt gctacaaaaa atcctaaaaa tgctccaatg aaaaggctag
    13681 gagcaaagac accaccatcg ccacctgaag ctaacgtagc tgatgtaatt attggtttta
    13741 aaatcaatat tcctataaat gtaactataa aagaaattga aaatgctgtt ttgccagaaa
    13801 taaatagtgc tttcatcgca tgatagccat caccatacaa ttgtggaagg ataaaaagta
    13861 atacacttat gataaatgaa cccaaaagaa ttttatgaaa atcattttca attttagaaa
    13921 attgtttttt gaaaaacaaa accattttag taagtaaaac agaattaaat ccggctaaaa
    13981 ttccaagtaa aataaaataa gggatggcat aataattcca acttttaatg attacagaaa
    14041 agagtggttc ttcgtctaaa atcaataaca agccataagc aaccgctagt gcaatactat
    14101 tggtgatgaa gaaaatcttg tttttctttt tagcaatgac ctccattgaa aaaagaattc
    14161 cagcaattgg gctactgaac aaaatagtta taccggcagt aattccagca caaattaatt
    14221 cagttttgta gtttttaaat aaattttctt ttttgtgagt agcagcacca atagccgctg
    14281 tagcaacaac cgttgaaact tcaattccag ttgaaccacc aaaagctact gtaaataatc
    14341 cgtttataaa atgtgaaggc actttgtata aaggtaaact tttatttttg gaatttgtag
    14401 catcaaagat ttctttaata cctttatttt ctttcttttt aaataaatag tgacgcaaaa
    14461 aatgaataac aaaaaaacca attataggaa aaacaaacat taaaaaattt tgttttgtag
    14521 cttttaaaag aaaactcagt tcaagatatt cagtaatgtg ttttaaagaa atggctaaaa
    14581 atgaagaaag aatacttatt aatattgctg caacaattaa tttttggaat aaaataatgt
    14641 tgaaatattt tcgaattgta gtgaatgtca ttataaaatg ataagaattg caaaattatg
    14701 caatcctttt tttaaaaaca ttatttttta tcttaagact atcctaaaag ttgaaacact
    14761 aaaccttaaa attgaaccaa aaagttgtat ttttgcactc caaaatagca agaaaatatt
    14821 atgttagaaa gattgcaata tgtaaaacaa cgatttgacg aagtatcaga tttgattatt
    14881 cagcctgatg ttattgccga tcaaaaacgt tatgttcaat taaatcaaga atataaaggt
    14941 cttaaagact tgatggaaaa gcgtgaagaa ttaatgctta ttacggcaaa tattgaagaa
    15001 gccaaggaaa ttattgcgga tggaagcgat gcagaaatga cggaaatggc aaaaatgcaa
    15061 ttggatgaag cgcaagaccg tttgccagaa ttggaagatg aaatcaagtt tatgttgatt
    15121 ccaaaagatg ctgaagatgc taagaatgtt atggtagaaa ttagagctgg aactggtggt
    15181 gatgaagctt ctatttttgc tggagatttg ttcagaatgt acaccaaata ttgtgaaact
    15241 cgtggctgga gaacttctgt agtagatatg aatgaaggaa cttcgggtgg ttttaaagaa
    15301 attatttttg aagttaccgg tgaagatgtt tatggaacat taaaatttga agctggtgtt
    15361 catcgtgtac aacgagtgcc acaaacagaa actcaaggaa gagttcatac ttctgctgcg
    15421 acagtaatgg ttttgccaga agcagaagag tttgatgttc aaattgatat gaatgatgtt
    15481 cgtgttgatt ttttctgttc ttctggtcct ggtggacaat cagtaaatac tacaaaatca
    15541 gctgttcgtt tgacacatat cccaacagga ttggtagcac aatgtcaaga tcaaaaatcg
    15601 caacataaaa acaaggacaa agctttagaa gttttgcgtt ctcgtttata tgaacaagag
    15661 ttagccaaaa aacaagcaga ggatgccgta aaaagaacat cacaagttag ttcgggtgac
    15721 cgttctgcaa aaattagaac atacaattat tctcaaggtc gagtaaccga tcatagaatt
    15781 ggattggatg tttttgatat ggacggcgtg atgaacggta aaattcaaaa atttgttgat
    15841 gaacttcaat tagtcaataa tatggaaaaa ttaaaagaaa gcgaagtttt ttaatttaca
    15901 cactttttag gatagtgtcg gaatattgta atatagtata aaaaaaccat ttcaattgaa
    15961 atggtttttt gtttaaactt aaaatctatt tagaacaaca tatcattatc ttctactatt
    16021 tcctctcgaa cttgacccag aagtactact tcttgaactg gagccacttg aaacacttct
    16081 tgaactcgat aaatttctcg aagtactaga gtggcttcta acacttgaat tactattcga
    16141 acttcttgta ggttgtgttc taatactctc attggttgat gaacctatac ttccattatt
    16201 tctagttgga gcaatttctc taacggtagt gtttctaaca ggtgtttcct gtttaacaga
    16261 tactgaagaa ttactgcttc gcacaccgcc agtagtagtg ttttctctaa ctgttcctgt
    16321 atttcttgtg ttattgcttc taacgctgct cgaattgtca gtagctctag tactattaaa
    16381 atcgttttta gaatttctta tgttgtttga ggctatttca tctctagtac caacagttcc
    16441 aacagttcta atgtttctgg ttttgtccaa ttcatatcta ttgacataac cactatgtct
    16501 gttggtaaaa gaacggtttg gatgttgcac ttcatacgca tttccacgtc ttcctctata
    16561 ataattgttg taaacagcat aacaatttct agtgtttaca tagttataat ggtggttaaa
    16621 gttaatccaa acattaatat tatttctata tctaaagatt ggaaacggat gccaagctac
    16681 ataataggat gggtaatagc cccaatacca actagaatag taaggtctgt aacgtggtgc
    16741 ccaaaaggta gtatatatta ctggtgtata tgcataaaca ggctcataga tatagttggt
    16801 tccatacata taaacatcac caactacttg tacttgtacc ctactgtctc tgtcttttgt
    16861 aacttcaact gtagcaatat cttgaaataa atctcttcca agcaccgatt gaattataat
    16921 taaatgaaca tttccttcta cggtttcaat tactctcaag taatctactt tattatcatt
    16981 attcaaatcc aaattcgata tttggtattc aggattgttc aatctatatt caaaatcatc
    17041 caaatcttta gagtctccaa agatagatgc cacagctctt aaatcaagat tatcactaat
    17101 ctcagaattg ttagccgtta ccgttgttct gtcttgagca aatgcaaagt ttaaactcat
    17161 cagcgacagc attgtcaaag taataatatt tgttttcatg atgctatatt ttatggattg
    17221 tatgttattg caaaatatcc aattattatg ccacaatttt ttagagttta tgaaataaaa
    17281 taattaatgt atttttagca aaactttaag agtatgaaaa actcattcat ctattggact
    17341 ttcctttgtc taattggt
//
LOCUS       DAOOBH010000039        17276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_4294_length_17276_cov_6.546542, whole genome shotgun sequence.
ACCESSION   DAOOBH010000039 DAOOBH010000000
VERSION     DAOOBH010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 17276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17276
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4294_length_17276_cov_6.546542"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..957
                     /locus_tag="PK218_05790"
     CDS             184..957
                     /locus_tag="PK218_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98053.1"
                     /translation="MKHIKRKFLFHDDLQKIDIPKKFEYFKKDEVNQQLLKKNAKDKF
                     VILETETNEKIFSFIYDNNGKHCMIPVPDFSLVNYNFAYTLNIERKEHIKKLIENLAD
                     LTTPNELSNTYAFDFQGCASSCIICLFTAIECFVNDIIPEKFIYQIKTQRKTEVYNKE
                     QIQFSISFMDKLTKVLPEAFNKNFFSKPTPTNAHIINLKDLRNNIVHTKSDRTGKNNV
                     EILKRLLSFKYDETLEATFKLFNFYREGFIEECSCNHDW"
     gene            1120..2355
                     /locus_tag="PK218_05795"
     CDS             1120..2355
                     /locus_tag="PK218_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HPW98054.1"
                     /translation="MKQKEKSAAIGFIFITMLIDITGWGIIIPVIPKLIAELIQGDIS
                     EAAKYGGWLGFAFAFTQFLFAPLVGNLSDKYGRRPIILISLFAFALDYLLLAFSPTIT
                     WLFVGRIIAGLSGASITTASAYIADISTPENRAKNFGMIGAAFGLGFIIGPVLGGLLG
                     QYGSRVPFYAAAILCMCNFLYGYFILPESLSKANRREFDWKRANPIGAIKRLKKYPQL
                     IGIVVAIFLLYVGSHAVHSNWSFYTMYQFKWDERMVGISLGVVGLLVGIVQGGLIRWT
                     SPRLGNEKSIYIGLALYTIGMLLFAFATESWMMFVFLIPYCLGGIAGPALQAVVSGNV
                     PANEQGEIQGTLASLMSASAIIGPPMMTNTFYFFTHKEAPFEFAGAPFILGGFLMLLS
                     TIIAFYALRRKAIVSTSNG"
     gene            complement(2330..2767)
                     /locus_tag="PK218_05800"
     CDS             complement(2330..2767)
                     /locus_tag="PK218_05800"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HPW98055.1"
                     /translation="MKDLHEILRKEKYKKIKFKVSKTQHLLVKAKINGVQGNFILDTG
                     ASNSCVGFESIEHFALIAGKSKTKAAGAGATGMFTQLAKDNKLQLGRWKTHDLHLVIF
                     DLSHVNEALQQYKAKRVHGIIGADILLEGKAIIDYFNHCLYLQ"
     gene            2862..3632
                     /locus_tag="PK218_05805"
     CDS             2862..3632
                     /locus_tag="PK218_05805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPW98056.1"
                     /translation="MILTDTHTHLYSEEFQNDRAEMIQRAIDNGVSRFFVPSIDSSYT
                     EKMYALETQFPENIYLMMGLHPCYVKPETYEQEIAHVEQQLASRKFQAVGEIGIDLFW
                     DKTTLPLQQDAFKRQIQLAKHYKLAINIHCRDAFDEVFEVLEDEKSDDLFGIFHCFTG
                     NLDQAERAIDLGMKLGIGGVVTFKNGKIDQFLHEVDLKHIVLETDSPYLAPIPYRGKR
                     NESSYTVLVAEKLAQIYQLPIEEIAQITSKNSKAIFGI"
     gene            3696..4832
                     /locus_tag="PK218_05810"
     CDS             3696..4832
                     /locus_tag="PK218_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPW98057.1"
                     /translation="MTKFDAIRPFYDTEVNEAICSCVNNPMMKAIMGFTFPDVDEEIW
                     KEQLKRTHSIRDFQCNFIYQSVKKVLEKSSEGLSTSGFEKLEKNTSYLFISNHRDIIL
                     DTSLLNVCLFDHGLVMTASAIGDNLVQKDFLKTLSRLNRNFLVQRGLSPRELLESSKL
                     MSEYIGQLLLRENRSVWIAQREGRTKDGNDATHSGVLKMLGMGSDEKNLMDYFKKLKI
                     VPVSISYEYDPTDALKMPQLMAAANAEKYIKEKNEDFITLLSGIIGQKKHIHIHVGNV
                     LDKELDEICSQFDNNNKQIQALTQALDDSILKNYKLWPTNYIAYDLLYNTTNYSNYYT
                     EAEKSLFERRLEMRIDDKNAQMVESFLAMYANPVVNKMKYENAF"
     gene            4822..5844
                     /locus_tag="PK218_05815"
     CDS             4822..5844
                     /locus_tag="PK218_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPW98058.1"
                     /translation="MLSKSNILLIYTGGTIGMMKDFETGALKAFNFKKLLQKIPELKQ
                     LECSIETLSFKKPIDSSNMNPNEWIKLATIIEENYTNFDGFVILHGSDTMSYSAAALS
                     FMLENLTKPVVFTGSQLPIGDLRTDAKENLITAIQIASSREKNKPVIQEVCLYFEYKL
                     YRGNRTTKISAEHFNAFASPNFPSLAESGVHLNFNRIEQKKTTKKLLVHKKLDDNVVI
                     IKLFPGINEAVLSSIIDIPNLKAIILETYGSGNAPTEKWFINLLKKGIKKGLYFVNVT
                     QCSGGSVNMGKYETSTELKKLGIISGKDITTEAAITKLMYLLGQNVSPAVFKTIFETS
                     LRGEMQ"
     gene            5900..5986
                     /locus_tag="PK218_05820"
     tRNA            5900..5986
                     /locus_tag="PK218_05820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5934..5936,aa:Ser,seq:gga)
     gene            6011..6718
                     /locus_tag="PK218_05825"
     CDS             6011..6718
                     /locus_tag="PK218_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPW98059.1"
                     /translation="MKKYIFILFFIVTATTSAQNKDAKQVEHDFDAIDSLYREDQFYF
                     SVSYCLMQSKPDGFQQNKFSPGMSLGFLRDMPINKNRTLALAAGIGYTYSTYNQNIVI
                     ISSNQNYEYQIAPDDVAISKDRFIFHTIDLPIEFRWRNSIPESTKFWRVYTGLKLSYL
                     FYDEYKLETSSGNVSISNNKDLNPFQFGVYVSAGWNTVNVFAYYGLNSFFKSGTKIDG
                     NPLDVNGIRFGLMFYIL"
     gene            complement(6713..7318)
                     /locus_tag="PK218_05830"
     CDS             complement(6713..7318)
                     /locus_tag="PK218_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98060.1"
                     /translation="MKKILPFFSYILHPVFIPLFGTLFYIILDENYFTFPQVLVLLLQ
                     IIIISILLPIAFFFLLRTFGKIDNIMLSDLSQRKIPLLLQIMLFSVLVTKSITLERFP
                     ELHYFFMGGIISSIVAFVLLYIKIKASIHLIGTSALTIFVLGLSYHNQVNIINTIIFI
                     MILNGFVASSRLEMKAHNTKELVFGFFVGALPQIGLLFFWL"
     gene            complement(7331..8785)
                     /gene="rpoN"
                     /locus_tag="PK218_05835"
     CDS             complement(7331..8785)
                     /gene="rpoN"
                     /locus_tag="PK218_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPW98061.1"
                     /translation="MLKQFLNLKLSQKLSPQQIQLMKLIQLPTQAFEQRLKEELIENP
                     ALESGADEEIYDKDDFDTQDDFEDNQSIEADEINIDEYLSDDETPDYKLQANNYSDDD
                     EDRTMPMAAQVSFHQDLINQLNTFILPENEREIAEFLVGSIDDDGYLRRTIQDVVDDL
                     AFTQGIYTDEKTVEKILHIIHELDPIGVGARDLQECLLLQLKHKTPSEYVDLAIDLIE
                     NQFEAFTKKHYDKLLQKYEISQEQLKKGIEEIEKLNPKPGGSFGGASRFIEQIIPDFT
                     IRIVDGELELTLNGRNAPSLHVSKDYQEMMQTYKDSSDKSHAQKDAVQFIKQKLDSAK
                     WFIDAIRQRQETLYVTMNAIMHLQQEYFLDGEETSLKPMILKDVADMVGLDISTVSRV
                     ANSKYVDTPYGTKLIKEYFSESMKNDQGEDVSTLEIKKILQNIVESEDKSKPYPDDQL
                     ADILKEKGYPIARRTIAKYRDLLDIPVARMRKKI"
     gene            complement(8877..10322)
                     /gene="asnS"
                     /locus_tag="PK218_05840"
     CDS             complement(8877..10322)
                     /gene="asnS"
                     /locus_tag="PK218_05840"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPW98062.1"
                     /translation="MKHSKVKDLLNDSRKDYEVTVKGWVRTFRNNQFIALNDGSTINN
                     LQCVVDFENTAEEILKRINTGTAVGITGNLVESKGAGQKYEIQVTQLEILGDCDAEKF
                     PMQPKKHSLEFLRENAHLRVRTNAFGAIMRVRSVLAHAVHTYFQERGFVYVNTPVITG
                     ADAEGAGEMFQVTSLPLDNLPKNEEGNIDYKKDFFGKHTNLTVSGQLEAETFAMALGQ
                     VYTFGPTFRAENSNTSRHLAEFWMIEPEVAFNDLDANMDLAEDFIQFVIKYTLEKCED
                     DLKFLEGRLLDEEKSKPQTERSEMSLLEKLNFVIENNFKRVSYTEAIDILRNSTPNKK
                     KKFQYIIEEWGADLQSEHERFLVEKHFKCPVILFDYPANIKAFYMRLNDNTEPGKETV
                     RAMDILFPGIGEIVGGSQREERFDVLVEKMKKLGIDEEELWWYLDTRRFGSAVHSGFG
                     LGFERLVLFVTGMTNIRDVIPFPRTPQNAEF"
     gene            complement(10365..12770)
                     /locus_tag="PK218_05845"
     CDS             complement(10365..12770)
                     /locus_tag="PK218_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW98063.1"
                     /translation="MKKKSTVGFWEFIAGIVLRRRISILIAITVFTIFLGLQWKNIHF
                     TYTEANLLPDDHQVNIEYNAFLKEFGEEGNLIVIGTKDQKLFTPKVFENWTKLMSDLN
                     KESEVELIVSVDNIKKLQKNDSLQTFEMVPFFDQTKVKDPNYLLATKKELFTKTPFYE
                     GLLYNKSGTVRSAIYLDKKIVNTPQRKDFILNKFIPRVEAFQKENNIDLKVSGMPYIR
                     TLNAKSILSEISLFIGAALFITSLIFYLFFRSHRATLISVLIVVIGVMWTFGFIGLFN
                     YEITILTGLIPSLVIVIGIPNCIFLTNKYQQEYAAHGNKARALQRVITRVGTATLMTN
                     LTTAAGFATFMITNSQLLKEFGLIAAINIIALFFLCLILIPIYYSYQPVPKAKHLEHL
                     NREYTQKFMDWIAKSVRYHRTKVYIVAVSLFLLSIIGALKIKTSGSLTEDMPKKAAFF
                     KDIQFFEEEFNGVMPLEITVDTKRKKGVMKSSTLRKIDELEKTIEKIPELSKPISVVN
                     LVKYSKQAFYNGNPEYFELPTAQEKSFVLSYAKNATKNSDKDLMKSYVSSDGSVARIT
                     TFMKDIDTGNMSRVEDELWKKINEIFPPEQYKVNLTGKALVFEKGTKYLLNNLVTSLL
                     FAVLLISLLMLFMFRSFKMVIVSLIPNLLPLLITAGLMGYFGIPLKPSTILIFSIAFG
                     LSVDDTIHFLAQYRQELTHHNWKIKKSVFATLKESGMSMFYTSVVLFSGFSIFMLSSF
                     GGTVALGGLIAVTLLFGMLSNLMLLPSLVLTLNKKLANEQEFIEPKIYILEREDKEEN
                     TSN"
     gene            12935..14125
                     /locus_tag="PK218_05850"
     CDS             12935..14125
                     /locus_tag="PK218_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPW98064.1"
                     /translation="MIGSIVITICVLMLLAYIFELTSSKTKIPSIILLLVLGWLVKQI
                     TVILHINIPNLEVALPVLGNLGLILIVLEGSLDLELKKSNYPIIAKSAIIALLPMVVM
                     SFGIAYAFYYFDPELTYKAALGNAIPLGVISSAVAISSVKNLNPTNKEIITYESSMSD
                     VLGVIFFNFVTLNQTIVASTFGYFFLDLILILIISFVATIGLAFLLSKIKHHVKFAPI
                     IILLIMIYFISKLYHLPALVFVLLFGLLISNLDEMTNLKFVQKLHPEILNKEVEKFKE
                     LNIEFTFLIRTLFFILFGYLIKTSEVLNTDTIIWAVGITAFIFLLRAIFLKVVRLPLK
                     PLFFIAPRGLITILLFLSIPITHRSKFMNNSLIIQVILLTVIVMMVGLMFNKKEKITS
                     QDQE"
     gene            complement(14103..16586)
                     /locus_tag="PK218_05855"
     CDS             complement(14103..16586)
                     /locus_tag="PK218_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPW98065.1"
                     /translation="MKNVFIFLLFCCLTMQAQQQITGIVKDFNTKKVLPFASIILNDD
                     QTFMADVDGKFEIVSFKKINSITIQYIGYSANTIEVSSKNYYPIFLKPKTDSLKMLTI
                     NSQNLANSILKKVIAKKEENNPQKKLDYFQFKAYNKIVVSANPDSIQGKIDSIFVENE
                     FGKELKKIDSTAYKFKKIIDKQHLFLAEKISKFQFAKPLVKETILASKMAGFKEPIYE
                     LFGFNLQSFSIYDDKYELFETYYKSPLSRNATDEYQYSISDTTSIENRETYVIYFKNK
                     KKKKGLEGLLFVDSQNFAVAKAVMQTKGIINITAIHEFRFLAENQVWFPIKKEFKITK
                     GKSDEKTSFLGGRIVFEPDDEDNEREKKNADFTFFSSETDIKDIKINEPIVLKKSFIK
                     VQVSDKNENRNEDFWEENRTFAIDERGIQTYKILDSIADDRKLDKKIRFGRKIINGYV
                     PISLFDFDLRHLFSYNNYEGFRFGLGGNTNEKLSKVYKIDGYTAYGLKDETIKYSIGS
                     AARIGKFSNTWIGYSYTDDLREIASTSFAIDKRVFKLYDPRPINISTFYNYKMWRGYI
                     ETKIIPKTESIWQLTYNNITPLFNYTFVHNGINYNHFEMTTAIASIQWNPFSDFMQTP
                     NGKLEIEKRFPKFTFQFTKSLPKLFDNDFDFGKIDLRIDFEKKYLNGQKSAILVQSGY
                     AYGAIPITHLYNTSPNNLNKTNLLQRITIAGKNSFETMFFNEFFSNQYVFFQLKHGFK
                     RVALFKKVKPEFVLVTRMAWGNLENPEHHLGIEYKTLDQGFFESGVELNQIFRGFGLS
                     GFYRYGPNQLNRLEDNLAIKLSFILDLGL"
     gene            complement(16684..17202)
                     /gene="frr"
                     /locus_tag="PK218_05860"
     CDS             complement(16684..17202)
                     /gene="frr"
                     /locus_tag="PK218_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPW98066.1"
                     /translation="MSGTIAHLEKEFLNIRAGKASPQMLGSVFVEYYGSQTPLSQIAN
                     VNVPDARTITITPYEKSMLQPIEKAIMVANLGFNPMNNGDNIIISVPPLTEERRRDLV
                     KQAKSEAEDAKIGIRNSRKDANTEIKKLEKEGTSEDICKSAEDDVQKLTDAFIKKIDE
                     HLVTKEAEIMKV"
BASE COUNT         5577 a         2729 c         2865 g         6105 t
ORIGIN      
        1 ctagaatata aacgttacgg atttggtggc ggacgtcgcc caacaataag tggagcccaa
       61 cttattatct ggaatgttga atttcttact ttattattaa gaaaagagtt ttgtgaagag
      121 gttttttttt atatttgaga ggagttagtt taaaaattgg ttattaatta atacttaaaa
      181 aacatgaagc atattaaaag gaaattttta tttcatgatg atttgcagaa aattgatatt
      241 ccaaaaaaat ttgagtattt taaaaaagat gaagtaaatc aacagttatt aaaaaaaaat
      301 gctaaagaca aatttgtaat attagaaact gaaactaatg aaaagatatt tagttttatt
      361 tatgataata atgggaagca ttgcatgata cctgttccag atttttcatt ggtaaactat
      421 aattttgctt acactttaaa catagaaaga aaagaacata taaagaaatt aattgaaaac
      481 ctagctgatt taactacgcc taatgaatta agtaacactt atgcttttga ttttcaaggt
      541 tgtgctagtt cctgtataat atgtctattt acagctattg aatgttttgt taatgatatt
      601 atccctgaga aatttattta tcaaattaaa acacagcgca aaacggaagt atacaataaa
      661 gagcaaattc aattttcaat ttcatttatg gataaattaa cgaaggtact tcctgaagca
      721 tttaataaaa atttctttag taaaccaaca cctaccaatg ctcatattat taaccttaaa
      781 gatttaagga ataatattgt tcataccaaa tcagatagaa ctggaaaaaa taatgttgaa
      841 atattaaaga gattactaag ttttaaatac gatgaaacat tagaagcaac atttaaactt
      901 tttaattttt atcgagaagg ttttatagaa gagtgttcat gtaatcatga ttggtaaaaa
      961 aaattgaaat agaatctttt ttccagttaa cgaacaaatc ctttcatgtg ggtctaaata
     1021 aatttactag ttgttaatta ctgctatgaa atacttcctt tttcagcata acactattgt
     1081 gatagttttt tcgttaattt gcctttcagt taaaaagcca tgaagcaaaa agaaaaatcg
     1141 gcagcgatag ggtttatttt cattacgatg ttgatcgaca ttacgggttg gggtattatt
     1201 attcccgtaa ttccaaagtt gattgctgag ttgattcagg gtgacattag cgaagcggca
     1261 aaatatggcg gttggcttgg ttttgctttt gcgtttacac agtttttgtt tgcacctttg
     1321 gtaggaaatc tgagtgacaa atacggcaga cgaccgatta tcttaatttc gctatttgcc
     1381 tttgcgttag attatttact gttggccttt tcgcctacca ttacttggtt gtttgttggt
     1441 cgcattattg caggactttc tggcgcgagt attaccacag cttcggcgta tattgccgat
     1501 attagcacac ccgaaaacag agccaaaaac tttggaatga taggtgctgc ttttggttta
     1561 gggtttatta ttggtccagt tttgggtggc ttgttagggc aatatggctc gcgagtacct
     1621 ttttatgccg ccgctatatt atgtatgtgc aactttttat acggctactt tattttaccc
     1681 gaatcactat ctaaagcaaa ccgtagggag tttgattgga aacgcgccaa tcctattggt
     1741 gccatcaaac gattgaaaaa atacccacaa ttaataggca ttgttgtagc tatatttctg
     1801 ctctatgtgg gttcacatgc cgtgcacagc aattggagtt tttatacgat gtaccaattc
     1861 aaatgggatg aaagaatggt cggaatttcg cttggtgtag ttggtttact tgttggaatc
     1921 gttcaaggtg gactaatacg ttggacatcg ccaagattgg gtaacgagaa aagtatttac
     1981 atcggtttgg ccttatatac tatcgggatg ttgctgtttg cttttgctac cgaaagctgg
     2041 atgatgtttg tgtttttaat tccgtattgt ttgggtggaa ttgctggtcc ggctttgcaa
     2101 gcagtggttt caggaaatgt tcctgctaat gaacaaggcg aaattcaagg cacgttagcc
     2161 agcttgatga gtgcttcggc aatcattggt ccgccaatga tgaccaatac tttttacttt
     2221 ttcacacata aagaagcgcc gtttgagttt gccggtgcgc catttatatt aggtggattt
     2281 ttgatgttgt tgagtaccat tatagcattt tatgcgttgc gaagaaaagc tattgtaagt
     2341 acaagcaatg gttaaaataa tcgataattg cttttccttc caaaagaata tcggcaccaa
     2401 tgatgccatg aactcgtttg gctttatact gttgcaaagc ttcgtttaca tgcgataaat
     2461 caaaaataac taagtgtaag tcatgggttt tccatcgtcc gagttgtagt ttattgtctt
     2521 ttgccaattg cgtaaacatt cctgttgctc cagcaccagc agctttggtt ttcgattttc
     2581 cagcaataag cgcaaagtgt tcgatgcttt caaaacccac acagctattg ctcgcaccag
     2641 tatcgagaat aaagttacct tgcacaccat taatcttggc ttttaccaat aaatgttgcg
     2701 tttttgaaac cttaaattta atttttttat acttctcttt cctaagaatc tcgtgcaagt
     2761 ctttcatggt ttgtaaatga tgtcaaatgt atttcaaata tagaaaacaa actttgtaac
     2821 tttgcgactt taggatttta ttataatttg tgccttaaac aatgatttta accgatacac
     2881 atacccattt atattccgaa gagtttcaaa acgatagagc agaaatgatt caacgcgcca
     2941 ttgataatgg tgtttcacga ttttttgttc cttccatcga ctctagttat accgaaaaga
     3001 tgtatgcttt ggaaacacaa tttcccgaga atatatatct aatgatgggg ttgcatccat
     3061 gttatgtaaa gcctgaaacc tacgaacaag aaatagcgca tgttgagcaa caattggctt
     3121 caagaaagtt tcaagccgtt ggcgaaattg ggattgattt gttttgggat aaaactacgt
     3181 tgccactgca acaagatgcg tttaaaagac aaattcaatt agcaaagcat tacaaattgg
     3241 caataaacat tcattgtcgc gatgcttttg atgaagtttt tgaagtgtta gaagatgaga
     3301 aaagtgacga cttatttgga atctttcatt gttttacagg caatttggat caagctgaaa
     3361 gagcaataga tttagggatg aagctcggaa ttggcggagt ggttaccttc aaaaacggaa
     3421 agatagacca gtttttgcat gaagtcgatt taaagcatat tgttctcgaa acggattcgc
     3481 cttatttggc accaattcct tatcgtggca aacgaaatga aagcagttat accgttttag
     3541 tagctgaaaa attagcacaa atctatcagc ttcccataga agaaatagct caaattactt
     3601 ccaaaaacag caaagcaata tttggaatat aaaaggtttt taaactaaaa gtcatatttt
     3661 ttttgttctt ttgttaattg aaaaaattga aaaaaatgac caagttcgat gccattagac
     3721 cattttatga tactgaagta aatgaagcca tttgttcgtg tgtcaataat cctatgatga
     3781 aggcaattat gggttttact ttcccagatg ttgatgaaga aatttggaaa gaacaattaa
     3841 aaagaacaca ctctattaga gattttcaat gtaattttat ttatcaatcg gtaaaaaaag
     3901 tactagagaa atcctcagaa ggattgtcca cttcgggttt tgaaaaacta gagaaaaaca
     3961 cttcttatct ttttatctcc aatcatcgcg atattatttt agatacttct ttgcttaatg
     4021 tgtgtttgtt cgaccatggt ttagtcatga cggcatctgc tattggagat aacttggtgc
     4081 aaaaagattt tctaaaaacg ctatcgcgac tcaatcgtaa ctttttagtt caacgaggtt
     4141 tgtcgccaag agaattgcta gagagttcaa agttaatgtc ggaatacatc ggacaactgt
     4201 tgcttcgtga gaaccgttct gtctggatag cgcaacgcga aggaagaaca aaagatggga
     4261 acgatgcgac acattctgga gttttaaaaa tgctaggaat gggttcggat gaaaaaaatc
     4321 ttatggatta tttcaaaaaa ctaaaaattg ttccagtttc tatttcgtat gaatatgacc
     4381 ctacagatgc attgaaaatg cctcaattga tggcagcagc caatgctgaa aaatacatta
     4441 aagagaaaaa tgaagatttc atcaccttgt taagcggtat tattggacaa aaaaagcaca
     4501 ttcacattca tgttggcaat gttttagata aagaattaga tgaaatatgc agccaatttg
     4561 acaacaataa caaacaaatt caagcattaa cccaagcttt agacgattcg attttgaaga
     4621 attataaatt gtggccaact aattatattg cctacgattt gttgtataat acaaccaatt
     4681 actcaaatta ttacaccgaa gcagaaaaat cattatttga acgcagattg gaaatgcgaa
     4741 ttgatgataa aaatgcacaa atggtagaaa gttttctagc aatgtatgcc aatccagtgg
     4801 ttaataaaat gaaatacgaa aatgctttct aagtcaaata tattattgat ttatactggt
     4861 ggaacgattg gtatgatgaa agattttgaa acaggagctt taaaagcatt caattttaaa
     4921 aaattgttgc aaaagattcc cgaattaaaa caattggaat gctctataga gaccctatca
     4981 tttaaaaagc ctatagattc gtccaacatg aaccctaatg aatggataaa attggccaca
     5041 atcattgaag aaaattacac aaattttgat ggatttgtca ttcttcacgg ttccgatact
     5101 atgtcgtatt ccgcagcagc tttaagtttt atgcttgaaa acttgactaa accagtagta
     5161 ttcacgggtt cgcagttgcc aataggtgat ttacgaacag acgccaaaga aaacttgatt
     5221 acggctattc aaattgcttc ttcaagagaa aaaaacaaac ctgttattca agaagtttgt
     5281 ttgtattttg aatacaaatt ataccgagga aatagaacta caaaaataag tgccgaacat
     5341 tttaatgctt ttgcgtcacc caattttcca tctttggcag aatcaggtgt acatttgaat
     5401 tttaatagaa tagaacaaaa aaaaactact aaaaaactgt tggttcataa aaagctagat
     5461 gataatgttg taattataaa attgtttcca ggcattaatg aagcggtttt gtcatctatt
     5521 attgatatac ccaatttgaa agcaattata ttagaaacct atggttctgg aaatgcacct
     5581 actgaaaaat ggtttatcaa tttactgaaa aagggaataa aaaaagggtt gtattttgtt
     5641 aatgtaaccc aatgttcagg aggaagtgtc aatatgggaa aatatgaaac cagcacagaa
     5701 ttaaaaaaac tgggtattat ttcgggaaaa gacattacta ccgaagcagc catcacaaaa
     5761 ttgatgtatt tgttaggtca aaatgtatct cctgctgttt ttaaaaccat ttttgaaact
     5821 tctttgagag gcgaaatgca ataagcaata aaatcgtttt ttttcttcaa aaattttatg
     5881 gttctttgca acaccaaaaa gagaggtgtc cgagtggttg aaggagctag cctggaaagc
     5941 tagtatgtgg gtaactgcat cgtgggttcg aatcccatcc tctctgcaaa tcattcattt
     6001 ttaaaatgtt atgaaaaaat atatcttcat tttgttcttt attgttactg caacaactag
     6061 tgcgcaaaat aaagatgcca agcaagttga acatgatttt gatgcgatag attctttata
     6121 tcgtgaagat caattttatt ttagtgtttc ctattgcctg atgcaaagca aacctgatgg
     6181 gtttcaacaa aacaagtttt ctcccggaat gtcgcttggg tttttaagag acatgcccat
     6241 caacaaaaac agaactttag cattggctgc tggtatcgga tatacatata gcacttacaa
     6301 tcaaaatatt gttataattt cgagcaatca aaattatgaa tatcaaattg cacccgacga
     6361 tgttgctatt tcaaaagata gatttatttt tcataccatt gatttgccca ttgaatttag
     6421 atggcgtaat tctatacctg aaagcactaa gttttggcgt gtatatacag gtttgaaatt
     6481 gagttattta ttttatgatg aatacaaact tgagacttcc tcgggcaatg ttagtatctc
     6541 aaataataaa gatttgaatc catttcaatt tggtgtttat gtttcggcgg gttggaatac
     6601 agtcaatgtt tttgcctatt atggattgaa ttcattcttc aaatcgggaa caaaaattga
     6661 tggaaatccg cttgatgtta atggaattag gtttggttta atgttttata ttttataacc
     6721 aaaaaaagag taatccaatt tgtggtaacg ctccaacaaa aaaaccaaaa actaattctt
     6781 tcgtattgtg tgccttcatt tctaatcggg aagaagctac aaatccattc aaaatcataa
     6841 taaaaataat agtgttgata atgtttactt ggttatgata acttaaacca agcacaaaga
     6901 tggtcaacgc acttgttccg attaaatgga tgctagcttt tatctttata taaagtaata
     6961 caaatgcaac aatcgaactg ataattccac ccataaaaaa atagtgtagc tctgggaatc
     7021 gttctaatgt tattgatttt gttactaaaa ccgagaacaa cataatttgt aacaacagtg
     7081 gaatttttcg ttgtgataag tcggaaagca taatgttgtc aattttacca aaagttctga
     7141 gtaagaaaaa aaatgcaatc ggaagtaaaa tactaatgat aataatctgc aaaagcaata
     7201 ctaaaacttg aggaaaagta aaataatttt catcaagaat gatgtaaaat agtgttccaa
     7261 acaatggaat aaaaacggga tgtaaaatgt acgaaaaaaa aggaaggatt tttttcaaaa
     7321 caatttagtg ttagattttt tttcgcattc tagctacagg aatatccaat aaatctctat
     7381 attttgctat ggttcttcta gcaattggat agcctttttc ttttaatata tctgctaatt
     7441 gatcgtcagg ataaggttta cttttgtctt ctgattcgac tatgttttga agaatttttt
     7501 taatttctaa agtagaaaca tcttcacctt gatcattttt cattgattct gaaaagtact
     7561 ctttaatcag ttttgtgcca tatggtgtat caacatattt gctgttggca acacgagaaa
     7621 cggttgaaat atccaaacct accatgtcag caacatcttt taaaatcatt ggtttcaggc
     7681 tggtttcttc gccgtcaagg aaatattctt gttgcaaatg cataatggca ttcattgtta
     7741 catataaagt ttcttgacgt tggcgaatag catcaataaa ccattttgct gaatctaatt
     7801 tttgtttaat aaactgaaca gcatcttttt gggcatgcga tttatcacta gaatctttat
     7861 aggtttgcat catttcttga taatctttag aaacgtgcaa tgatggtgca tttcttccgt
     7921 ttaaggtcaa ctccaactcg ccatccacaa ttctaattgt aaaatcggga ataatttgtt
     7981 caataaatcg ggaggcacct ccaaatgaac caccaggttt gggatttaat ttttcaattt
     8041 cttcaatccc tttttttaac tgttcttgag aaatttcata tttctgcaat agcttatcgt
     8101 aatgcttttt agtaaaagct tcaaattgat tttctatcaa atcgattgcc aagtccacgt
     8161 attcacttgg tgttttgtgt tttaattgca ataaaagaca ttcttgcaaa tctcttgcgc
     8221 caacaccaat tgggtctaat tcgtgaataa tatgaagaat tttttcaacc gttttttcat
     8281 cggtataaat tccttgcgta aatgccaaat catctacaac atcttgaatc gttcggcgta
     8341 aataaccatc atcatctata cttcctacca gaaattcggc aatttctcgc tcgttctcgg
     8401 gtaaaataaa agtatttaat tgattaatca aatcttgatg gaaacttact tgtgccgcca
     8461 ttggcatggt tcggtcttcg tcgtcatcac tataattatt ggcttgtaat ttgtaatcag
     8521 gtgtttcgtc gtcgcttaaa tattcgtcaa tgttgatttc atctgcttcg atagattgat
     8581 tgtcctcaaa atcatcttgg gtgtcaaaat cgtctttatc ataaatctct tcatcagcac
     8641 cactttccaa agctggattt tctatcaatt cctctttcag ccgttgttca aatgcttgtg
     8701 taggcaattg tatcaacttc atcaactgaa tttgttgagg tgatagtttt tgtgataatt
     8761 ttaaatttaa aaactgtttt aacatttgtt ttttgtttaa aggtttaagc gtttaaaagt
     8821 ttaagattgt tgctgcctga aacaacttta aactatatta aacaatttta aactttttag
     8881 aattctgcat tctgaggagt tcttgggaaa ggaattacat ctctaatgtt agtcattcca
     8941 gtcacaaaaa gaactaatcg ttcaaaacct aatccaaaac cagaatgaac tgctgaacca
     9001 aatcttcgtg tgtctaaata ccaccaaagt tcttcttcat caatgcctaa tttcttcatt
     9061 ttttctacca aaacatcaaa gcgttcttct ctttgagaac cgccaacaat ttcgccaatg
     9121 cctggaaaaa gaatgtccat agcacgaacg gtttcttttc ctggttctgt attgtcgttt
     9181 aaacgcatat aaaatgcttt aatgtttgca ggataatcaa ataagattac gggacattta
     9241 aagtgttttt ctaccaagaa acgttcgtgt tcactttgta aatcggcacc ccattcttca
     9301 ataatatatt ggaatttctt ctttttattt ggcgtagaat ttcttaaaat atcgatagct
     9361 tcggtatagg aaacgcgttt gaaattattt tctattacaa agttaagttt ttctaacaaa
     9421 ctcatttcac ttcgttctgt ttgtggtttt gacttttctt cgtctaataa tcttccttct
     9481 agaaatttca aatcatcttc acatttttcc aacgtatatt ttattacaaa ttggataaaa
     9541 tcttcagcta agtccatatt agcatctaaa tcgttgaaag ccacttcggg ctcaatcatc
     9601 caaaactcag ctaagtgacg cgatgtattt gagttttcag cacggaatgt tggtccgaaa
     9661 gtatatactt gacccaatgc catagcaaaa gtttcggctt ctaattgtcc agaaaccgtt
     9721 aagtttgtat gttttccgaa gaaatctttt ttataatcaa tgtttccttc ttcatttttt
     9781 ggcaagttat ctaatggcaa tgaagttact tgaaacattt cgcctgcacc ttccgcatca
     9841 gcacccgtta ttacaggtgt atttacatag acaaatccac gctcttgaaa ataggtgtga
     9901 accgcatgag ctaataccga acgaacacgc ataatggctc caaaagcatt ggttctaaca
     9961 cgtaaatggg cattttctct aagaaattct aaagaatgtt tttttggttg cattggaaat
    10021 ttctcagcat cacaatcgcc aagaatttca agttgcgtca cttggatttc atatttttga
    10081 ccagcacctt tgctttcaac caaatttcca gtgataccaa cggctgttcc ggtattaatt
    10141 ctttttaata tttcttcggc tgtattttca aaatcaacta cacattgaag attatttatc
    10201 gttgaaccat cgttcaacgc tataaattga ttgtttctaa aagttctaac ccagcctttt
    10261 acggttactt catagtcttt tcgtgagtcg tttagtaagt ctttaacttt tgaatgcttc
    10321 atttttatca aaattttgta atgcaaatat aatttaaatc ggtgttaatt ggaagtgttt
    10381 tcttctttgt cttcgcgttc caaaatgtaa atttttggct cgataaattc ttgttcgttg
    10441 gctaattttt tattcaatgt caaaactaaa ctaggaagta acatcaaatt tgacaacatt
    10501 ccgaaaagca gcgttaccgc aatcaatcca ccaagtgcta ctgttcctcc aaagctagac
    10561 aacataaaaa ttgaaaaacc agaaaacaaa accaccgaag tgtaaaacat gctcattcca
    10621 gattctttca acgtggcaaa aacagatttt tttattttcc aattgtgatg ggtaagttct
    10681 tggcgatatt gggctaaaaa gtggatggta tcatctacgg aaagtccaaa ggcgatgctg
    10741 aaaataagaa tagttgatgg ttttaacgga atgccaaaat agcccattaa cccagcggtg
    10801 attagtaatg gtaataaatt tggaattaaa gacactatta ccattttaaa cgaacgaaac
    10861 atgaatagca ttaataagga aatcagtaaa acagcaaata ataatgaagt tactaaatta
    10921 tttagtaaat attttgttcc tttttcaaaa actaatgctt ttccggtcaa gttgactttg
    10981 tattgttcgg gtggaaatat ttcgtttatt tttttccaaa gttcgtcttc aacgcgcgac
    11041 atgtttcctg tatcaatgtc tttcataaaa gtagtaatac gtgcgacact accatctgaa
    11101 ctaacatagc ttttcattaa gtctttgtct gaattcttgg tggcattttt ggcatagctc
    11161 aatacaaaac ttttttcttg tgctgtgggc aattcaaaat attcaggatt tccattatag
    11221 aatgcttgtt tggaatattt taccaagtta acaacagaaa taggttttga aagttcgggt
    11281 attttttcaa tggttttttc taattcatca attttccgaa gtgttgacga cttcatgacg
    11341 cctttttttc ttttggtgtc aacggttatt tctagtggca tcacaccatt aaactcttct
    11401 tcgaaaaact gaatgtcttt aaaaaaggct gcttttttag gcatatcttc tgttaaactt
    11461 ccagaggttt tgatttttaa agcacctatg atgcttaaaa gaaataaaga aacagcaaca
    11521 atgtaaactt tggttctatg ataacgtaca cttttagcaa tccagtccat gaatttttga
    11581 gtgtattctc tattcaagtg ctccaaatgt ttggcttttg gtacaggttg atagctataa
    11641 taaataggaa ttaatatcaa gcataaaaaa aacaatgcaa tgatatttat ggcagcaata
    11701 agtccaaact cttttagtaa ttgactgttg gtaatcataa aagtagcaaa accggctgca
    11761 gtagtaagat ttgtcattaa tgttgccgtt ccaactcggg taattactcg ttgaagagca
    11821 cgggctttgt taccatgcgc agcatattct tgttggtatt tattggttag gaaaatgcaa
    11881 ttcggaattc caataacaat aactaaagat ggaattaacc ccgttagaat tgtaatttca
    11941 taattgaaca atccgatgaa accaaaggtc cacattacac caattacaac tattaaaaca
    12001 gaaattaaag tggcgcgatg cgaacggaaa aatagataaa aaattaacga agtaataaaa
    12061 agagctgccc caataaacaa acttatttcg ctcaaaatac ttttagcgtt tagtgttctt
    12121 atgtaaggca ttccagaaac ttttaaatca atgttgttct ctttttgaaa agcttcaact
    12181 ctcgggatga acttgtttaa aatgaagtct ttgcgttgtg gtgtgtttac aatttttttg
    12241 tctaaataaa ttgcagaacg aacggtgcca cttttattat aaagtaaacc ttcatagaat
    12301 ggtgtttttg taaaaagttc ttttttggtg gccaataaat aattagggtc ttttactttt
    12361 gtttgatcaa aaaacggaac catttcaaaa gtctgtaaac tatcgttttt ttgcagcttc
    12421 ttgatgttgt caacagaaac aataagttca acttctgatt ctttgtttaa atcagacatc
    12481 aattttgtcc aattttcaaa tacttttgga gtaaataatt tttggtcttt ggtgccaata
    12541 acaattaggt taccttcttc tccaaattct tttagaaaag cgttgtattc aatatttact
    12601 tggtggtcat ctggcaacag attggcctca gtataggtaa aatgtatgtt tttccattgt
    12661 aaaccaagaa aaatggtaaa aacagtaatg gcaattagga ttgagattct tcttctaagt
    12721 acaattccgg caataaattc ccaaaatcct actgttgatt tctttttcat gataaattta
    12781 aaacggttgc aaaggtaatg aaaacaagag aataagcgtt aattttacca ttgttttagc
    12841 taaaaagaat tcacgataaa gtaatttaag aatttattat tttataaaaa aaaaggttac
    12901 atttgaaaat aatcccaaaa atgcgttctc ctttatgatt ggatctattg ttattactat
    12961 ttgtgtgcta atgttgttag cctatatttt tgaattaacc tcttcaaaaa caaaaattcc
    13021 ttcaataatt ttattgttgg ttttgggttg gttagtaaaa caaattacgg tcattctaca
    13081 tattaatatt ccaaatcttg aagttgcact accagtattg gggaatctgg gattgatttt
    13141 aattgtatta gaaggatcat tagatttaga actaaaaaaa tcgaattatc caattatagc
    13201 caaatcagct attattgcac tgttgccaat ggttgttatg agttttggaa tagcgtatgc
    13261 gttttattat tttgatcctg agttgaccta taaagcagct ttaggcaatg ccattccgct
    13321 aggcgtaatt agtagtgctg tggctatttc aagtgtaaaa aacttaaatc caacgaacaa
    13381 agaaatcata acttatgaaa gcagtatgtc ggatgtttta ggtgttattt ttttcaattt
    13441 tgttactcta aatcaaacaa ttgttgcttc aacttttggt tattttttcc tcgatttaat
    13501 attgatttta attatatcat ttgtagcaac aatcggtttg gcttttttgt taagtaaaat
    13561 taaacatcat gtaaaatttg ctcctataat cattttactg ataatgattt atttcatttc
    13621 aaaactatat catttaccag cattagtgtt tgtcttattg tttggtttgt tgattagtaa
    13681 tttggacgaa atgacaaatt taaaatttgt tcaaaaattg catcctgaga ttttaaacaa
    13741 ggaagtagaa aagtttaagg aattgaatat tgaatttaca tttcttatta gaacactatt
    13801 ttttattctt tttggatatt taatcaaaac atccgaagtg ttgaataccg atactataat
    13861 ttgggcagtt ggaataacag cattcatttt tttattgaga gcaattttct taaaagtagt
    13921 tcgattacca ttaaaaccat tgttttttat tgcaccacgc ggattgatta caattctttt
    13981 gtttttgtct attccaataa cacatagaag taaatttatg aataactctt taattattca
    14041 ggttatttta ttgaccgtaa tagttatgat ggttggattg atgtttaata aaaaagaaaa
    14101 aatcacaagc caagatcaag aataaagctc aactttatag ctaagttatc ttccaatcta
    14161 ttcaattgat ttgggccata acgataaaat ccagaaagtc cgaatcctct aaaaatttga
    14221 tttagttcga ctcccgattc aaaaaagcct tggtctaagg tcttgtattc aatgcctaaa
    14281 tgatgttcag gattttctaa atttccccaa gccattcgag ttactagaac aaattcgggt
    14341 tttacttttt tgaaaagcgc tactcgttta aacccgtgtt ttaattgaaa aaacacatat
    14401 tgattggaga aaaactcatt aaaaaacatc gtttcaaaac tgttttttcc tgcaatagta
    14461 atgcgttgta gtaagttggt tttgtttaaa ttgttaggcg aagtgttata aagatgtgtt
    14521 ataggaatgg caccataggc atacccagat tgtaccaaaa ttgctgattt ttgaccgttt
    14581 aaatattttt tctcaaagtc aattcgcaaa tcaattttcc caaaatcgaa gtcgttatca
    14641 aatagttttg gcaacgattt tgtaaactga aaagtgaatt ttggaaagcg tttctcaatt
    14701 tctaattttc catttggtgt ttgcataaaa tcactaaacg gattccattg tatagaagca
    14761 atagccgttg tcatttcaaa atggttataa tttatgccat tatgaacaaa agtatagttg
    14821 aataaagggg taatgttatt ataggttaac tgccaaatac tttctgtttt aggaataatt
    14881 tttgtttcaa tataaccgcg ccacatttta taattgtaaa atgtactgat gttaattggt
    14941 cgtggatcat ataatttaaa aactcttttg tctatagcaa agcttgtact ggcaatttct
    15001 cgcaaatcgt cggtatagga ataaccaatc caagtgtttg aaaacttacc tattcttgca
    15061 gcagaaccaa tgctgtattt tatagtttca tctttcaaac cataagcagt ataaccatca
    15121 attttataga ctttcgatag tttttcatta gtgtttccgc ccaatccgaa acgaaatcct
    15181 tcataattat tgtaactaaa aagatgtctt aaatcaaaat cgaataggga tattggtaca
    15241 tatccattga ttatttttct tccaaagcga atttttttgt ccagttttcg atcatcagca
    15301 atactgtcta gaattttata ggtttgtatt cctctttcgt caattgcaaa agttctattt
    15361 tcttcccaaa aatcttcgtt tctgttttcg tttttgtcac ttacttggac tttaataaac
    15421 gactttttta gaacaattgg ttcgtttatt tttatatctt taatatctgt ttctgaacta
    15481 aaaaaagtaa aatctgcgtt ctttttttct ctttcattgt cttcatcatc aggctcaaaa
    15541 actattctac cgccaagaaa tgaagttttc tcatcgcttt ttcctttggt tattttaaac
    15601 tcttttttta tgggaaacca aacttgattt tctgctaaaa aacgaaattc atgaatagct
    15661 gtaatgttga taattccttt tgtttgcatc accgctttcg caacagcaaa attttgagaa
    15721 tcaacaaaca acaatccttc taatcctttt ttcttctttt tgtttttaaa ataaatgaca
    15781 taagtttctc tgttttctat tgaagtggta tcactaatag aatactgata ttcatctgtt
    15841 gcatttcttg aaagtggact tttataatag gtttcaaaca gttcatattt gtcgtcataa
    15901 attgaaaaag actgaagatt aaaaccaaat aattcgtaaa ttggctcttt aaatcctgcc
    15961 atttttgagg ctaagatagt ttcttttaca agaggttttg caaattgaaa ttttgaaatt
    16021 ttttctgcca aaaacaagtg ttgcttatca ataattttct tgaatttata cgctgtggaa
    16081 tcaatttttt ttaactcttt cccaaattcg ttttcaacaa aaatactgtc aatttttcct
    16141 tgtatagaat ctggatttgc tgaaactact attttgttgt aggccttaaa ctgaaagtaa
    16201 tctagctttt tctgtggatt gttttcctct ttttttgcaa taactttttt gagtatcgaa
    16261 ttggctaaat tttgactatt gatagtaagc atttttaaac tatccgtttt aggttttaaa
    16321 aaaattggat aataattttt tgaagaaact tcaatagtat ttgcagaata tcctatgtat
    16381 tgaatggtta tagagttgat ttttttaaaa gatacaattt caaatttacc atctacatca
    16441 gccataaatg tttggtcatc gtttagaatg atgctggcaa aaggcaacac tttttttgtg
    16501 ttaaagtctt ttacgattcc agtaatttgt tgctgtgctt gcatagtcaa acaacaaaat
    16561 aaaagaaaaa taaaaacgtt tttcatcaaa gtaattttta caaaagtagg tataaaaaaa
    16621 atccgtcaag tttaacttaa gaagttttta actagacgga ttttatagag gtatttataa
    16681 aagttatact ttcataattt ctgcttcttt tgttaccaag tgttcgtcta ttttttttat
    16741 aaatgcatcg gttagttttt gaacatcgtc ttctgccgat ttacaaatat cttccgaagt
    16801 gccttctttt tctaattttt taatttcagt attggcatct tttcttgaat ttcgaatacc
    16861 aattttggca tcctcggctt ctgattttgc ttgttttact aagtcgcgtc ttcgctcttc
    16921 cgttaaagga ggaacactga taataatgtt atcaccatta ttcattgggt taaagcctaa
    16981 attggcaacc ataattgctt tttcgattgg ttgcaacatg cttttttcat atggtgtaat
    17041 agtgatggtt cttgcatcag gaacattcac atttgcaatt tgacttagtg gtgtttgaga
    17101 accataatac tctacaaaaa cactacccaa catctgtggc gacgcttttc ctgctctaat
    17161 attcaaaaat tctttttcta aatgcgcaat tgttccactc atggattctt ttgtgctaaa
    17221 aaaaattcta tttcttccat ttttataaag tttaaacatt taaggattta aaagtt
//
LOCUS       DAOOBH010000040        17096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_4362_length_17096_cov_5.645913, whole genome shotgun sequence.
ACCESSION   DAOOBH010000040 DAOOBH010000000
VERSION     DAOOBH010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 17096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17096
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4362_length_17096_cov_5.645913"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PK218_05865"
     CDS             <1..203
                     /locus_tag="PK218_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HPW98067.1"
                     /translation="GKKATQNPWKSNTLEWTTPVEHIHGNWPGEIPEVHRWPYDYSKP
                     GHDEDFVPQNVPMKPGEEVLHH"
     gene            283..1305
                     /gene="ruvB"
                     /locus_tag="PK218_05870"
     CDS             283..1305
                     /gene="ruvB"
                     /locus_tag="PK218_05870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPW98068.1"
                     /translation="MNENLNPTNENFNPEELDLEKKLRPLSFVDFTGQEQVLDNLKVF
                     VEAANLRDEALDHTLFHGPPGLGKTTLANILANELGVGIKITSGPVLDKPGDLAGLLT
                     NLEERDVLFIDEIHRLSPIVEEYLYSAMEDFKIDIMIESGPNARTVQINLNPFTLVGA
                     TTRSGLLTAPMRARFGIQSRLQYYNTELLTTIVQRSSSILKMPITMEAAIEIAGRSRG
                     TPRIANALLRRVRDFAQIKGNGSIDIEIARFSLKALHVDAHGLDEMDNKILTTIIEKF
                     KGGPVGLSTLATAVSESGETIEEVYEPFLIQEGFIMRTPRGREVTEKAYKHLGKIKGL
                     NQGELF"
     gene            1302..2228
                     /gene="queG"
                     /locus_tag="PK218_05875"
                     /pseudo
     CDS             1302..2228
                     /gene="queG"
                     /locus_tag="PK218_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
     gene            2540..4840
                     /locus_tag="PK218_05880"
     CDS             2540..4840
                     /locus_tag="PK218_05880"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPW98069.1"
                     /translation="MHHKDTKRREALLYHAKPTPGKIKVVPTKKYATQRDLSLAYSPG
                     VAEPCLEIAKDVNNVYKYTSKGNLVAVISNGTAVLGLGDIGPEASKPVMEGKALLFKI
                     FADIDVFDIEVDTKDIDAFVETVKNIAPTFGGINLEDIKAPESFEIERRLVEELNIPV
                     MHDDQHGTAIISSAALLNALFLAKKKIDKVKVVVSGAGSAALACANLYVLLGVRIKNI
                     IMFDKDGVLSSDRNDLSDLQKRYTSAKPGTDLKKALKDADVFLGLSAGNILTPEMLKG
                     MAKNPIVFAMANPIPEIDYDLAIATRKDIIMATGRSDHPNQVNNVLGFPYIFRGALDV
                     RATKINEAMKMAAVRALAALARESVPEQVNIAYGETKLNFGRDYIIPKPFDPRLISIV
                     APAVAKAAMDSGVALNPITDWQKYEEELLDRLGSDNKMVRLLTNRAKSSPKRVVFAEA
                     DHLDVLKAAQIVHEEGIAKPILLGNKEVILELKEELGFDADVLIIDPKTKEEDERRMH
                     YADIFWKARQRKGISLYEAQKWMRERNYFAAMMVNNGEADALVSGYSRSYPSVVKPMM
                     QLIGKAPGISLIATTNMMMTARGPMFLSDTAINPNPTSDDLAKIALMTEKTVRMFGME
                     PVIAMISYSNFGSSTNQTANTVREAVAYLHKYYPDLIVDGEIQADFALNPEMIKDKFP
                     FSKIAGKKVNTMIFPNLEAANITYKLLKELNHIDSVGPIMLGMDKPVHIFQLGASVEE
                     MVNMVCVAVVDAQEKEKRNKLIKVNF"
     gene            4918..5499
                     /gene="ruvA"
                     /locus_tag="PK218_05885"
     CDS             4918..5499
                     /gene="ruvA"
                     /locus_tag="PK218_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPW98070.1"
                     /translation="MIAHIQGKVVEKTPTEVIIDCNGVGYHINISLHTFSLLPATDFV
                     KLFTFLQIKEDSHSLFGFVEKSERELFKLLLSVSGIGASIARTMLSSLEPKQIIQAIA
                     VGDVATVQSIKGIGLKTAQRAIIELKEKVLKVYDLNEISTISHNINKEESLSALEVLG
                     YPRKSAEKVVDKIVRENPEATVETIIKQALKNI"
     gene            5503..>7172
                     /gene="sprA"
                     /locus_tag="PK218_05890"
     CDS             5503..>7172
                     /gene="sprA"
                     /locus_tag="PK218_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPW98071.1"
                     /translation="MKTNNITFSNLSGLLMLLFVVTFTNSFAQVTETPADSLGYNTGR
                     IDIANPNSVINAYKYDPATNRYIYSSTFEGFNINYPIILTPQEYDELVLRESMRDYFK
                     KKSDAIDGKKEGSEADKKDLLPRYYVNSGFFETIFGSNTIDVKPTGSVEMDLGVRYTK
                     QDNPAFSPQNRAQTSFDFNQRISMSLMGKVGTRLNVNANYDTQSTFAFQNLIKLEYAP
                     TEDDIIQKIEVGNVSMPLTNSLIRGAQSLFGVKAQLQFGKTTITGVFSEQKSQTKSVT
                     AQGGGTIQDAEIFALDYDADRHFFLSQYFRNKYDEALVNYPNIQSRVQITRIEVWITN
                     KQNRVNNTNNNLRNIIALQDLGEGDLSGVDVDKVVAIAAGDLPGFFVNATDAPPSNTN
                     NKYNPREIGTAGYLLNNNIRDVTTSSQGFNNTVPISEGNDYSKLENARKLNANEFTFH
                     PQLGYVSLQQRLANDEVLAVAYQYTIGDQVYQVGEFGTDGVESTVVSGSVDDPNISTQ
                     SLILKMLKSNLTNVQKPIWNLMMKNIYQIPGAYQLQQEDFRFNILYTDP"
     assembly_gap    7173..7272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7273..12887
                     /gene="sprA"
                     /locus_tag="PK218_05895"
     CDS             <7273..12887
                     /gene="sprA"
                     /locus_tag="PK218_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPW98072.1"
                     /translation="AYQLQQEDFRFNILYTDPSPINYISPANATSPLPANVAETALIK
                     VLNVDKLNYNNDPQNGGDGFFDFIPGLTVDQQNGRVIFTTVEPFGKLLFEKLRTSPGE
                     DYFENYNASSQLPTTNTFNDNQKKYVFREMYRTTQAAALQDAARNKFQLKIRYKSSGG
                     EGIPIGAFNVPRGSVVVTAGGRLLVEGVDYSVNYQAGRVQILDPSLQASNTPIEVSVE
                     NNSVFGQQTRRFMGLNVEHKFSNKFLLGATLLKMTERPFTQKSNYGQESVNNTIFGFN
                     GNYSTEVPFFTRLVNKLPNMDTDVPSNLSVRGEIAFLQPDASKNDQFGGESTIYVDDF
                     EGSQTTIDMRSPLAWSLSSVPKFPEMPEAGTDTYDFNASALDLSYGYKRAKLNWYSID
                     PTLYVQTPGDIGPDGISLNSTRRIFNSELFPNQELAIGQSQVVNTLDLTYYPAERGPY
                     NYEPAAATTGVLPNPADNFGGITRAINSTNFEQSNVEYIQFWVMDPYYPNTDPNAEPA
                     ANTGRVYFNLGEISEDILKDERKQYENGLPANTTSQALTSSTVWGKVPSAQSLIYAFD
                     VDGGNRASQDVGFDGLDDDGERNLPGIGAYASLPDPAADNYQYFLAATGSSIFERYKN
                     YNGVQGNSPVDVSDTNRGSTTLPDVEDINRDNTMNTINAYYEYKIDIQPNMNVGSNFI
                     TDVRETVAENLPNGNSIPVRWLQFKIPVKDYQNKVGSISDFRSIRFMRMFMTGFSQPI
                     TVRFGALDLVRGEWRRYDNSFQITDANPDDDGTNFDVLSVNIQENASRSPIPYVTPPG
                     VVREQIANGNSIVNQNEQSLSLRVSGDGLETGDSRAVFKNVSIDMRQFNKLKMFLHAE
                     ALPTDGTPLLDDQMSAFIRFGNDFTNNYYEVEIPLKVSAPTSKTDVEIWPAINDMDLR
                     TELLTKLKVIALSADPGDFVDGVLYKNDVELDPSLAGIANRGPLRIGIKGNPNFGLVR
                     TLMVGVRNRHDQKVRGEVWFNELRLADMSNKGGMAAILNVDTNFADFATISATGRRST
                     IGFGTLEQGPNERSREEVLQYNLVTNFGLGKLLPKKWGINLPFNYAIGEETITPEYDP
                     YNQDLRLQQLLDNTPDAAERANIENRALDYTRRKSINFIGVKKERSQEQKPHFYDPEN
                     LTLSYSYNEIKKQNYEIENFIDQQVNATVDYTFAFQPKPVEPLKNTKFLKKSDYWKLL
                     REFNFNYLPTSISYSTSIIRQFNKQRFRQVDVNGIPLDDLYRRNYMFNYQYGFNFNLT
                     KSLKLNYTASTRNLVRNYIDENYDVDNSYTVWTDFWNIGTPNQHNQQIVVNYDLPVNK
                     LPFLAFVKSTYSYTGDYNWQRASLALESIEGYNLGNTVQNAGSHRFNTAFNMETFYKY
                     IGLGKKAQAPTRPKTLPKPGEKITSTTQPSQSTNVFLDGLKGVLTSVKNVQINYTRNQ
                     GTMLPGFLPSIGFFGSSKPGLGFIFGDQADVRYEAARNGWLTSYPDFNQNFTRVKTTT
                     LDMTANVDLFPDFKIDLVANRTYADNYSEQYNVDPVTGFYNSQSPYTTGNFAMSTVML
                     KSAFKGSDENFSSSFNDFRGNRIIIADRLATSFYGNTTFARDADGYPVGFGKNSQAVL
                     LPSFLAAYTGLYTTGANDSAKDISLDAFRNIPIPNWTIKYTGLMRYKFFKDTFKRFSI
                     QHAYRASYTLNSFRSNLDYDRNNPNKVDAGGNIQLQTIIGNANLVEQFNPLIRVDFEM
                     KNSLKILTEMKKDRSLSLSFDNNLLTEVQGFEYIVGFGYRFKDVIFSSKLADNPTGII
                     KSDINMKIDFSYRNSKTIVRYLDYENNQIGNGQNIWSAKLTADYSFSKNLTAIFYYDH
                     SFSKPVISTSFPLTNIRAGFTLRYNFGN"
     gene            12964..13344
                     /gene="gcvH"
                     /locus_tag="PK218_05900"
     CDS             12964..13344
                     /gene="gcvH"
                     /locus_tag="PK218_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPW98073.1"
                     /translation="MNIPANLKYTKDHEWIAIDGDIATVGITDFAQKELGDIVYVEVE
                     TLDQTMDRDEVFGTVEAVKTVSDLFLPLSGEIIEFNDSLESSPETVNTDPYGDGWMIK
                     VKFSNPSEVAELLSSDDYKKLIGA"
     gene            13316..13723
                     /locus_tag="PK218_05905"
     CDS             13316..13723
                     /locus_tag="PK218_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPW98074.1"
                     /translation="MIIKNLLALKTFWLVTAVFWTSTILFLCLIDSSELPSLSIKMEG
                     TDKGVHFTFHFIFTLLWSMYVYSLTKNINRKKILKVLIASFLFGVLIEIFQGCFTTTR
                     QADVMDVLANTIGAISAGILIYFILKRIKKLKT"
     gene            13799..15925
                     /locus_tag="PK218_05910"
     CDS             13799..15925
                     /locus_tag="PK218_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein RemB"
                     /protein_id="HPW98075.1"
                     /translation="MRNYFSFLFLFIGLLVQAQNNIKVSERFPVFPECEGQQDAALET
                     CFNNQVHDFVYTTFKVPEELTTKNYKGTIIVLFEVTDEGKFIVQYVDANEKALVEESK
                     NVFEKMPKISPATYNGKPTYAKYTLRITIPLQSIAQIQAEKEAAAEAAKKSTTYVKNT
                     KELNEFEAVKYQEFDQPQFESHNNIPLSHSYYAQFDDELNQVGANNHTSSKPFAYAEV
                     NKYYNFKEAYKAIEKPKTTWLGKKFWNENLVELKGDDYWFTLNPIVDIQLGKSQSNVT
                     DNSKSTFINTRGIQFNGGLGKTVNFTTTLYESQGRFAAYFNRYAESIKPDGGNPAIIP
                     GIGIAKAFKTDAYDFPLAEANLAFTPSNFFNLNLGYGRNFIGDGYRSLLLSDGASPYP
                     YIKVNTTFWKIKYTNIYTWLKDVRPEVIVDKTYATKYVASHYLSMNVSKRLNIGFFES
                     VVWTNQNNRGFDMSFVNPIIFYRAVEFASSSRSGNALLGLTTKYKFNNQINFYGQFLL
                     DEFSLNDIKAGKKSWKNKFGYQLGAKYYNAFNVKNLMLQLEYNHVRPYVYAHSDPITN
                     YGHNNQSLGHNWGGNFKEVVAIARYFKGRYFADAKITFGERGLDFNTPDNNFNYGGNI
                     YLDYDIDRPFDTGVNVGQGNKTTILIADLQAGYLINPSTNMKLFGSLIFRNFSPAAET
                     LTTFKENTTWFSLGLRCDIFNWYFDY"
     gene            complement(16182..16385)
                     /locus_tag="PK218_05915"
     CDS             complement(16182..16385)
                     /locus_tag="PK218_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98076.1"
                     /translation="MKLELKRVCIYPKDVQRITGKSYRHARLLLIEVKKHFNKEPHQL
                     VAIEEFCSYTGLQLEHVLPLIQG"
     gene            16660..>17096
                     /locus_tag="PK218_05920"
     CDS             16660..>17096
                     /locus_tag="PK218_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98077.1"
                     /translation="MARQKGIIKIKGTLGDITFYKSQDGYLVKEKSGVDGKRIATDPA
                     FQRTRENGSEFGRAGKAGKALRMAFRPILIKCADNRMVGRLTQAMIKVLQADAVSERG
                     LRNVIDGETELLVGFEFNIRGKLSNSLYAPYAAQIDRVTGELT"
BASE COUNT         5701 a         2691 c         3255 g         5349 t
ORIGIN      
        1 acggtaagaa agcaactcaa aacccatgga aatcgaatac tttagaatgg actacaccag
       61 tggagcatat tcatggtaac tggcctggag aaattcctga agtgcacaga tggccatatg
      121 attatagtaa accaggtcat gatgaagact ttgttcctca aaatgttccg atgaaacctg
      181 gtgaagaagt gttacatcat taattcaact tagatttaaa aaatgccttt cattgttgaa
      241 aggctttttt atttacatct atctttattc tatatttgta ttatgaatga aaatctaaac
      301 ccaactaatg aaaacttcaa tccagaagaa cttgatcttg agaaaaagtt aagacctctt
      361 tcatttgttg atttcaccgg acaagaacaa gttttggata acttaaaagt gtttgttgaa
      421 gcagctaatc ttagagatga ggctttagac cataccttgt ttcatggtcc tcctggttta
      481 gggaaaacta ctttagctaa tatacttgct aatgaacttg gtgttggcat caaaattact
      541 tcgggaccag tattggataa accgggtgat ttggcgggtt tgttaaccaa tcttgaagaa
      601 agagacgtat tgtttattga tgaaattcat cgcttgagtc ctattgtgga agagtatttg
      661 tattctgcta tggaggattt taagattgac atcatgatag aatcgggacc aaatgctaga
      721 acggttcaaa ttaacttgaa tccatttaca ttggttggtg ctactactcg ttctggattg
      781 ttgacggcac cgatgcgtgc tcgttttggg atacaaagcc gacttcaata ttataacacg
      841 gaattgttaa ctaccattgt tcaacggagt agtagcattc taaaaatgcc aattactatg
      901 gaagctgcca ttgaaatcgc gggtagaagc agaggaacac ctcgtattgc taatgcatta
      961 ttgagacgtg ttcgtgattt tgctcaaata aaaggaaacg gaagcattga tattgaaatt
     1021 gctcgttttt cactcaaagc tttgcatgtt gatgctcatg gtttggacga aatggataat
     1081 aaaattctaa ctactatcat tgagaagttt aaaggcggac cagttggttt gtcaactttg
     1141 gctactgcgg tttctgaaag tggtgaaact attgaggaag tttacgaacc tttcttaatt
     1201 caagaaggat ttatcatgag aactcctcgt ggaagagaag tgactgagaa ggcttataag
     1261 catttaggaa aaataaaagg attgaatcaa ggcgaattgt tttgataaat aataaagaaa
     1321 aatacacatc ctttattaaa gccgaagcta ctcgcctcgg ttttttgtct tgtggtgttt
     1381 ctaaggctgg ttttttggag gaggaagcac ctcgattgga acgatggttg accaataatc
     1441 accagggcga aatgggttat ttagaaaatc attttgataa gcggttgaac ccaaccttgc
     1501 tggttgatgg tgctaagagt gtggtgtctt tgttgttgaa ttattatcct cctgaacttc
     1561 aaaatggaga aagttataag atttctaaat atgcttacgg caaggattat cattcggtta
     1621 ttaataagaa attgaagaag ttgcttcgaa gtattaaggc agagattggc gaggtgaatg
     1681 gtcgtgcttt tatggatatt gcaccagtga tggacaaggt ttgggcggct aaaagtggct
     1741 tgggctggat gggtaaacac agtaatttga ttactcaaaa agtgggttcg ttttatttta
     1801 ttgcagaact tattttggat ttgtaattgg attacgataa tcccactacg gatcattgtg
     1861 gttcgtgcac agcgtgtatt gatgcttgtc ctactgaggc gatagtggca ccgtatgtgg
     1921 ttgatggcag caagtgtatt tcgtattata ctattgagtt gcagaataat attcctattg
     1981 aggccaaggg taagctggat gattggatgt ttgggtgtga tgtgtgccag gatgtttgtc
     2041 cgtggaataa gttttctaaa gcgcataatg agcctttgtt ttctccttat cctgagttac
     2101 tttctttttc taagaaagat tgggaagaga tttcagagga aacttttgct aaggtgttta
     2161 ctaattcgcc attaaaacga gctaaactcg aaggcttaaa gaagaatatt gcttttctaa
     2221 aagaataagt taggttagtt tataaaccat taaaatacat tgagatgatt aagattcttg
     2281 gattgtgttt ataggtacaa atccgatact tgggttaaat atatatatat atatatatat
     2341 aaatggtttt tgggtttttt tgaactaatt taaaaacata gggcattaac aaaatgatta
     2401 caatgtctaa tcctttataa tctgtatcga tactttagaa cgagtctttt tcaataaata
     2461 tttcaaaaac aaaccgctta aaaaatttcc tttttaaagt tagtcttcta cctttgtaag
     2521 ttcgaataaa aaagaaacta tgcatcataa agatacgaag cgaagagagg cacttttata
     2581 tcatgccaaa ccaacaccag ggaagataaa agtggttcct actaaaaaat atgctacgca
     2641 aagagatttg tctttggcgt attcgccagg ggttgctgag ccttgtttag agattgctaa
     2701 agacgttaat aacgtttata aatatacatc aaaaggaaac ctagtggctg taatttctaa
     2761 tggaacggct gtgcttggat taggcgatat tggtcccgaa gcgtctaaac ctgtgatgga
     2821 aggaaaggca ttgttgttta agatttttgc tgacattgat gtttttgata ttgaagtaga
     2881 tacgaaagat attgatgctt ttgtagaaac agtaaaaaat attgcaccta cttttggagg
     2941 aataaacctt gaggatatta aggcaccaga gtcgtttgaa attgaaagac gattggtgga
     3001 ggagttaaat attcctgtga tgcatgatga tcaacatggc acggcaatta tttcgtcggc
     3061 agctttgttg aacgcattgt ttttggcaaa aaagaagatt gataaagtaa aagtagtggt
     3121 ttctggtgct ggttcagcgg ctttagcttg cgctaacttg tatgtactgt tgggtgttcg
     3181 gattaagaat ataatcatgt ttgataaaga tggtgtgtta tcttcggata gaaatgattt
     3241 gtctgattta caaaaaagat atactagtgc aaaaccaggc actgatttga aaaaagcact
     3301 taaggatgct gatgtgtttt taggactttc ggcaggaaat attttgacac cagaaatgct
     3361 aaaaggcatg gcgaagaatc cgattgtttt tgcgatggcg aatccgattc cagaaataga
     3421 ttatgattta gcaattgcta ctcgaaaaga tattatcatg gctacgggtc gttctgacca
     3481 ccctaaccaa gtgaataacg tactagggtt tccttatatt tttcgtggtg cgctagatgt
     3541 tcgagcgaca aaaataaacg aagcgatgaa aatggctgcg gttagagctt tggccgcatt
     3601 ggctagagaa tcggtaccag aacaagtgaa catagcttat ggcgaaacta aattaaactt
     3661 tggtagggat tatattattc cgaaaccatt tgatccaaga ttgatttcga tagtggcgcc
     3721 agctgttgca aaagcggcaa tggattctgg agtagctttg aatccaataa ccgattggca
     3781 gaaatatgaa gaggaattgt tagaccgttt aggttctgac aataagatgg ttcgattgtt
     3841 aaccaataga gcaaaatcga gtccgaaacg tgttgtattt gccgaagcgg atcatttgga
     3901 tgttttaaaa gcggctcaaa ttgtgcatga agaaggcatc gctaaaccga ttcttttagg
     3961 aaataaagag gttattttgg agctaaaaga agaattagga tttgatgcag atgtattgat
     4021 tatagaccca aaaacaaaag aagaagacga aagaagaatg cattatgccg atatcttttg
     4081 gaaagcaaga caacgaaaag ggatttcatt gtatgaagct cagaaatgga tgcgcgaacg
     4141 caattatttt gcggcgatga tggttaataa tggtgaagca gatgctttgg tttcgggata
     4201 ttcgagaagt tatccatctg tagtaaaacc aatgatgcaa ctgattggaa aagcaccagg
     4261 aatctcgttg attgctacta ccaatatgat gatgactgct cgtggaccaa tgtttttgtc
     4321 agatacggca attaatccta atccaacttc tgatgacttg gcaaaaattg ctttaatgac
     4381 tgaaaagaca gtacgcatgt ttggaatgga accagtgatt gcaatgattt cctattctaa
     4441 ctttggttct tctacaaacc aaacagctaa tactgttcga gaagcggtgg cttatcttca
     4501 caaatattat cctgatttaa ttgttgatgg cgaaattcaa gctgattttg ctttgaatcc
     4561 agagatgatt aaagataaat ttcctttttc taaaattgct ggaaaaaaag tgaacaccat
     4621 gatatttcca aatttagaag cagcaaacat tacatacaaa ctcttaaaag agttaaatca
     4681 tatcgactct gttggaccaa taatgcttgg aatggacaaa ccagtgcata tttttcaatt
     4741 gggagccagt gtggaagaaa tggtaaacat ggtttgtgta gccgtagttg atgctcaaga
     4801 gaaagaaaaa agaaacaaac taatcaaagt taatttttga tttatttaac attattccaa
     4861 agtaacacta tgaattaaag aattttgtta tatttggaaa tacatttttt tgtaataatg
     4921 atagcccata ttcaaggtaa agtagtagaa aaaacaccaa cagaagtaat aatagattgt
     4981 aacggagtag gttatcatat taacatttca ctccatacgt tctcattgct tcctgctact
     5041 gattttgtaa aactatttac gtttcttcaa attaaagaag attcgcattc cctttttgga
     5101 tttgtagaaa aatctgaaag agaattattt aagttgttgc tttcagtttc aggtattggt
     5161 gctagtattg cccgaacgat gctttcttct ttagaaccta agcaaattat acaagcaata
     5221 gccgttggcg acgttgctac tgtgcaatct ataaaaggaa ttggactaaa aacagcccaa
     5281 agagctatca ttgagttaaa agaaaaagtg ttaaaagtgt atgatttaaa tgaaatttct
     5341 acaatttcac acaatattaa caaagaagaa tcgttatctg ctttagaggt tcttggttat
     5401 ccaagaaaat cagcagaaaa agttgtggat aaaattgtca gagaaaatcc agaagcaact
     5461 gtagaaacaa taatcaaaca agcgttaaaa aacatatagt acttgaaaac taacaatatt
     5521 actttttcca atttaagcgg tttgctaatg ttactttttg tagtaacgtt tacgaattct
     5581 tttgctcaag tcaccgaaac tccagccgat tcattaggat acaatactgg aagaattgac
     5641 attgctaatc caaatagtgt tattaatgct tataaatatg acccagcaac caatcgttat
     5701 atttattcta gcacctttga aggttttaat attaattatc caattatttt aacaccacaa
     5761 gaatatgatg aactggtgtt gcgagagtcg atgcgagact atttcaaaaa gaaatcagat
     5821 gcaattgatg ggaagaaaga aggaagtgaa gcggataaaa aagatttatt accacgatat
     5881 tatgtaaact ctggtttctt cgaaactatt tttggttcca ataccattga cgtaaaacct
     5941 acaggttctg tagaaatgga tttaggagtt cgatatacta aacaagataa cccagcattt
     6001 tctccacaaa atagagcgca aacttctttt gattttaacc aaagaatcag catgagtttg
     6061 atgggtaaag tgggaacacg attgaatgtt aatgccaatt acgatacgca atcaaccttt
     6121 gcttttcaaa acttaattaa actagagtat gctccaacgg aagatgatat tattcaaaaa
     6181 attgaagtgg gtaatgtgag catgcctttg acaaattcct taattcgtgg tgcacaaagt
     6241 ttatttggtg taaaagccca attacagttt ggaaaaacaa caataacagg agttttctct
     6301 gaacaaaaat cacaaaccaa gtcagtaact gcacaaggcg gaggaacaat tcaagatgct
     6361 gaaatttttg ctttggatta tgatgcggac agacactttt ttctttcaca atatttcaga
     6421 aataaatatg atgaagcttt agtcaactat ccaaacatac agagtagagt gcagattact
     6481 cgaattgaag tttggataac caacaaacaa aatagagtaa acaataccaa taataactta
     6541 agaaacatca ttgcacttca agatttaggt gaaggagatt tgtcgggagt agatgttgat
     6601 aaagtagtag caattgcagc tggagatttg ccaggatttt ttgtgaatgc aactgatgcg
     6661 ccaccaagca atactaacaa taaatacaac cctagagaaa ttggaactgc tggatatctg
     6721 ttaaacaata acattagaga tgttactaca tcaagtcaag gttttaataa taccgttcca
     6781 attagcgaag gaaacgatta ttcaaaactg gaaaatgccc gaaagttaaa cgcaaacgaa
     6841 tttacttttc atccgcaatt gggttatgtt tctttgcaac aacgtttagc caacgatgaa
     6901 gtgttggcag ttgcctatca atataccatt ggtgatcaag tatatcaagt tggagaattt
     6961 ggtacagacg gtgtagagtc aactgttgta agtggttcag tagatgatcc aaatatttca
     7021 acgcagagtt tgattttgaa aatgcttaaa agtaatttaa ccaatgttca aaaaccaatt
     7081 tggaacttga tgatgaaaaa catttatcaa attccgggtg cttatcaatt acaacaagaa
     7141 gattttagat ttaatatttt atataccgat ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nngtgcttat caattacaac aagaagattt tagatttaat attttatata
     7321 ccgatccatc gcctataaat tatatttcac ccgcaaatgc aacttcacca ttaccagcca
     7381 atgttgcaga aaccgcattg attaaggtgt taaatgtaga taagttaaat tataacaatg
     7441 acccgcaaaa tgggggtgat ggtttctttg actttatccc gggtttaact gttgaccaac
     7501 aaaatggtag agttattttt acaactgttg agccatttgg taaactgttg tttgaaaaat
     7561 taagaacaag tccaggagaa gattattttg aaaattacaa tgcctcctct caattgccaa
     7621 caactaatac ttttaacgac aaccaaaaaa aatatgtgtt tcgtgaaatg tatcgtacca
     7681 ctcaagctgc tgccttgcaa gatgccgcta gaaataaatt tcaattaaaa atcaggtaca
     7741 aatcgtctgg tggcgaaggt attccaattg gtgcatttaa tgttcctcga ggttcagtag
     7801 tagttacagc tggcggaaga ttgttggtag aaggagttga ctatagtgtc aattatcaag
     7861 ctggaagagt tcagatttta gatccatcgc tgcaagcatc aaatacacca attgaggttt
     7921 ctgtagaaaa caattctgtt tttggtcaac aaactcgaag atttatgggt ttaaatgtgg
     7981 aacataaatt ttccaataaa tttttgctag gcgcaacatt gttaaaaatg acggaaagac
     8041 catttactca aaaatcaaat tatggtcaag aatctgtcaa caatactatt tttggattta
     8101 atggaaatta ctctaccgaa gttcctttct ttaccagatt agtaaataaa cttcctaata
     8161 tggatactga tgttccttct aatttatcag tccgagggga gattgctttt ttgcaaccag
     8221 atgcttctaa aaatgaccag tttggtggtg aatctaccat ttatgtagat gattttgaag
     8281 gttcgcagac taccattgat atgcgttcac ctttagcatg gtcattgtca agtgtgccaa
     8341 aattcccaga aatgcctgag gctggaactg atacctatga ctttaatgcc agtgcactcg
     8401 atttgagtta tggctacaaa agagctaaat taaactggta tagtatagac ccaacattat
     8461 atgttcaaac accaggagat atagggcctg atggcatttc attaaacagt acaagaagaa
     8521 tatttaacag tgaattattc ccaaatcaag agttggctat tggacagtcg caagtggtca
     8581 atactttaga tttaacttac tatccagcag aacgtggacc ctataattat gaacctgctg
     8641 ctgcaactac aggcgttttg cctaatcctg ctgataattt tggtggaatt actagagcca
     8701 taaattcaac caattttgaa caaagtaatg tagaatacat tcaattttgg gtaatggatc
     8761 cttattatcc aaataccgac ccaaatgctg aaccagcagc taacacagga cgagtttatt
     8821 ttaacttagg tgagatttca gaagatattt tgaaggatga acgaaagcaa tatgaaaacg
     8881 gtttacccgc aaacactact tcacaagcac taaccagttc gacggtttgg ggtaaagtac
     8941 cttcggcaca atctttaatt tatgcttttg atgttgatgg aggaaataga gcttctcaag
     9001 acgttggttt tgatggtttg gatgacgatg gtgaaagaaa tttaccagga atcggtgctt
     9061 atgcttcatt gcccgatcca gctgcggata attaccaata ctttttagcg gcaactggaa
     9121 gttcaatttt tgaaagatat aaaaactaca atggtgttca aggaaattcg ccagttgatg
     9181 tttcagatac taatcgtggt tctaccactt taccagatgt tgaagacatt aatcgtgaca
     9241 atacgatgaa tacaattaat gcttattatg agtataaaat agacattcaa cctaatatga
     9301 atgttggatc aaacttcatt actgatgtta gagaaacagt agcagaaaat cttccaaatg
     9361 gaaattcgat tccagttagg tggttgcagt ttaagattcc cgtgaaagat tatcaaaata
     9421 aagtaggaag catttctgat ttccgttcca ttcgttttat gagaatgttt atgactggat
     9481 tttctcagcc aattacggtt cgctttggag ctttagattt ggttcgtggc gaatggagaa
     9541 gatacgacaa ctcattccaa ataaccgatg caaatccaga tgatgacgga acaaactttg
     9601 atgttctttc ggttaatatt caagaaaatg ctagcagaag tccaatacct tatgtaacac
     9661 cacctggtgt tgtaagagaa cagattgcaa acggaaacag tattgtaaat caaaacgaac
     9721 aatcgctatc attgcgtgtt tcgggtgatg gattagaaac aggagattct agagcggtat
     9781 ttaaaaatgt gagtatcgac atgcgtcaat ttaacaaatt gaaaatgttt ttacatgctg
     9841 aagctttgcc tacagatgga acacctttgt tagatgacca gatgtcagca ttcattcgtt
     9901 ttggaaatga ctttaccaac aactattatg aggtagaaat tccactaaaa gtatcggctc
     9961 ccacatcgaa aaccgatgtt gaaatttggc cagcaattaa cgatatggat ttaagaacgg
    10021 aactattaac aaaattaaaa gttatagctt taagtgctga tccaggcgat tttgtggacg
    10081 gtgttctgta taaaaatgat gtagaacttg atccttcatt agcaggaatt gccaatcgag
    10141 gaccattgcg aattggaatt aaagggaatc caaattttgg attagtacga acattaatgg
    10201 ttggtgttcg caaccgacat gatcaaaaag ttagaggaga agtttggttt aatgaacttc
    10261 gtttggcgga catgagtaac aaaggcggaa tggctgccat attaaatgtt gatactaact
    10321 ttgctgactt tgctactatt tctgctacag gtagaagaag tactattgga tttggaacgt
    10381 tagagcaagg accaaatgaa agaagtagag aagaagtgtt gcagtataat ttggttacca
    10441 attttggttt aggcaaatta ttaccaaaaa aatggggaat taatttacct tttaattatg
    10501 ccattggcga agaaacgatt acgccagaat acgatccata caatcaagat cttcgtttgc
    10561 agcaattgtt agacaataca cctgatgctg ctgaaagagc caatattgaa aatagagctt
    10621 tagattatac cagaagaaaa agcatcaatt ttattggtgt taaaaaggaa cgttctcaag
    10681 agcaaaaacc acatttctat gatcctgaaa acttgacgct ttcgtattcc tacaatgaga
    10741 ttaaaaaaca aaattatgaa attgaaaact ttattgacca acaagtaaat gcaacagttg
    10801 actatacttt tgcttttcaa ccaaaacctg ttgagccatt aaaaaacact aaatttttaa
    10861 agaaaagtga ttattggaaa ctactgagag agtttaattt taattatttg ccaaccagta
    10921 tttcttatag cacaagcatt attcgacaat ttaataaaca acgatttaga caagttgatg
    10981 taaatggtat tccattggat gatttatata gaagaaatta tatgttcaat tatcaatatg
    11041 gctttaactt taatttgacc aaatcattga agttaaacta taccgcttcc accagaaact
    11101 tagttcgaaa ctacatcgac gaaaactatg atgttgacaa cagttatact gtttggactg
    11161 atttttggaa tattggaaca ccaaatcagc ataaccaaca aattgttgta aattatgatt
    11221 taccagttaa taaattaccg tttttagcat ttgtaaaatc tacctattca tatacgggtg
    11281 attacaattg gcagcgtgct tctttggcat tagagtcgat tgaaggttat aatcttggaa
    11341 atactgttca gaatgctggt tctcaccgtt tcaatacggc atttaatatg gaaacctttt
    11401 ataaatatat aggtttaggc aaaaaagcac aagcaccaac acgaccaaaa acattgccaa
    11461 agcctggcga aaaaattacc agcactacac aaccctcaca atcaaccaac gtatttcttg
    11521 atggattgaa aggcgtatta acaagtgtga aaaatgtgca aattaattat actcgaaatc
    11581 aaggaaccat gttgcccggg tttttgccaa gtattggttt ctttggctca tcaaaacctg
    11641 gtttaggatt catttttggt gatcaagccg atgttcgtta tgaagctgca cgaaacggtt
    11701 ggttgacttc ttatcctgat tttaatcaaa actttacacg tgtaaaaact actactttag
    11761 atatgaccgc taatgttgat ttgtttccag atttcaaaat tgatttggtt gccaatagaa
    11821 cttatgcgga taattattcg gaacaatata atgttgatcc agtaacgggt ttctataact
    11881 ctcagtcgcc atatactact ggaaattttg ctatgtcaac cgtaatgctt aaatctgctt
    11941 tcaaaggaag tgacgaaaat ttctcttcat cgtttaacga ctttagagga aatagaatca
    12001 tcattgccga tagattagct acatcatttt atggcaatac aacttttgct cgtgatgccg
    12061 atggatatcc agttggtttt ggaaaaaata gtcaagccgt gcttttacct tcttttttag
    12121 ctgcctatac tggtttatat accactgggg ctaatgattc tgcaaaagat atttcattag
    12181 atgcttttag aaatattcct attccaaact ggacgattaa atatactgga ttgatgcgtt
    12241 ataaattctt taaagatact tttaaacggt tttctatcca gcatgcttat agagcttctt
    12301 atactctaaa ctcattccgt tctaatcttg attatgatag aaacaatcca aataaagtag
    12361 atgctggtgg taatattcaa ttgcaaacta ttattgggaa tgccaacttg gtagaacaat
    12421 ttaatccatt gattcgtgtg gattttgaaa tgaaaaactc cctgaaaatc ttgactgaaa
    12481 tgaagaaaga tagaagtctt tcgctaagtt ttgataataa tttactaacc gaagtacaag
    12541 gctttgaata cattgttggt tttggttatc gatttaaaga tgtcattttc tcttctaagt
    12601 tggccgataa tccaacggga attattaaaa gtgatatcaa tatgaaaatt gatttctctt
    12661 atagaaatag taaaacgata gtacgttatt tagattatga aaacaaccaa attggaaatg
    12721 gtcaaaacat ttggtctgca aaattaactg cggattattc gtttagtaaa aacctaaccg
    12781 ctattttcta ttacgaccat tcattctcaa aacctgtaat ttctacctct ttcccattaa
    12841 cgaatattag agcaggattt acattgcgtt ataattttgg aaactaaaaa aattttaaag
    12901 ttttcatttt aataatcgct caagaaataa tacttttgga taaattaata aacacaaaac
    12961 caaatgaaca tacccgctaa tttaaaatac actaaagacc acgaatggat tgctattgat
    13021 ggcgatattg ctactgtagg cattaccgat tttgctcaaa aagaattggg agacattgta
    13081 tatgttgaag tagaaacact tgatcaaacc atggatagag acgaagtatt tggaactgtg
    13141 gaagccgtaa aaaccgtttc cgatttattt ttaccacttt cgggcgagat tattgaattt
    13201 aatgatagtt tagaatctag cccagaaaca gtaaatactg atccttatgg cgatggttgg
    13261 atgataaaag taaaattttc taatccaagt gaagtagccg aacttttatc tagcgatgat
    13321 tataaaaaac ttattggcgc ttaaaacatt ttggcttgta acggctgttt tttggacaag
    13381 cacaatactt tttttatgtt tgatagatag tagcgaacta ccttctttga gtattaaaat
    13441 ggaaggaacg gataaaggtg ttcattttac ttttcatttt atattcacgt tgctttggag
    13501 tatgtatgtt tattcattaa ccaagaacat caatagaaaa aaaattctaa aagtactcat
    13561 agcttctttt ctttttggtg tgttgattga aatttttcaa ggatgcttta ctactacacg
    13621 tcaagcagat gtaatggatg ttttagcgaa tactattggt gcaatatcgg ctgggatatt
    13681 gatttatttt attctaaaac gaattaaaaa attaaaaaca taataaaaag tcccatttcg
    13741 atgggatttt tttattttta tagcttgaaa agtgaattct aatttattgc tgcctcctat
    13801 gagaaactat ttctccttct tatttctgtt tattggacta ttggttcaag cacaaaacaa
    13861 cattaaagtg tctgaaagat ttcctgtttt tcctgaatgt gaagggcaac aagatgcagc
    13921 attagaaact tgttttaata atcaagtgca tgattttgta tatactactt tcaaggttcc
    13981 agaagaattg actactaaga attacaaagg aactattatc gttttgtttg aagtaacaga
    14041 tgaaggaaaa ttcattgtac aatatgttga tgccaatgaa aaagcactag ttgaagaaag
    14101 taaaaacgta ttcgaaaaga tgccaaaaat tagtccggca acttataacg ggaaaccaac
    14161 gtatgcaaaa tatactttac gaattactat tcctttacaa agcatagccc aaattcaggc
    14221 agaaaaagaa gctgctgccg aagctgccaa aaaatcaact acctatgtta aaaacacaaa
    14281 agaactcaat gagtttgaag ctgtaaaata ccaagaattt gaccaacccc aatttgaaag
    14341 tcacaacaac ataccgttat cacacagtta ttatgcacaa tttgatgatg aattaaacca
    14401 agttggtgcc aataatcata ccagttcaaa accttttgcc tatgctgagg tcaataaata
    14461 ttacaacttt aaagaagcct ataaagccat cgaaaaacca aaaaccactt ggcttggtaa
    14521 aaaattctgg aatgaaaatc tagtcgaact taaaggtgac gactattggt ttaccctaaa
    14581 tccaattgtg gatattcaat tggggaaaag tcaatctaat gtaaccgaca attcaaaatc
    14641 tacctttatc aatactcgtg gtattcaatt taatggtgga ttgggcaaaa ccgtaaattt
    14701 taccacaaca ttatatgaaa gtcaaggacg ttttgcggct tattttaacc gatatgctga
    14761 atcaataaaa cctgatggtg gcaatcctgc cattataccc ggaataggaa ttgcaaaggc
    14821 gttcaaaacc gatgcctatg attttccttt ggcagaagcc aatttagcct ttacaccaag
    14881 taatttcttt aatctaaatc ttggttacgg acgcaatttc ataggtgatg gttatcgttc
    14941 tcttttgttg agcgatggcg caagtccata tccttacatt aaagttaata ccactttttg
    15001 gaaaataaaa tataccaaca tttatacttg gctaaaagat gttcgaccag aagttatagt
    15061 agataaaacc tatgctacca aatatgttgc cagtcactac ttgagcatga acgtttccaa
    15121 acgattgaat atagggtttt ttgaatccgt agtgtggacc aatcaaaaca atcgcggatt
    15181 tgacatgagc tttgtaaacc caatcatttt ctaccgtgct gttgagtttg cttcgtcttc
    15241 tcgaagcgga aatgccttac tggggttaac cacaaaatat aaattcaaca atcaaattaa
    15301 cttctatgga caattcttgc tggatgagtt ttcgctaaat gatattaaag caggtaaaaa
    15361 aagctggaaa aataaattcg gataccaact tggtgctaag tactacaatg cgttcaatgt
    15421 aaaaaacttg atgctacagt tagaatacaa ccatgttcgt ccttatgtct atgcacacag
    15481 tgatcccatc accaattacg gacacaacaa ccaaagtctt ggtcacaatt ggggtggaaa
    15541 cttcaaggaa gttgtggcta tcgcacgtta ctttaaaggc agatactttg ccgatgccaa
    15601 aattactttc ggtgaacgtg gattggattt taatacaccc gacaacaact tcaattatgg
    15661 tggaaacatc tatttagatt atgatattga ccgaccattt gatactggag taaacgtagg
    15721 acaaggaaat aaaaccacta ttttgattgc tgatttacaa gcaggttatt tgattaaccc
    15781 atctacaaac atgaaacttt ttggtagtct tatctttaga aactttagcc cagcagctga
    15841 aacattaacc accttcaaag aaaacaccac ttggttttct cttggtttgc gttgcgatat
    15901 ttttaattgg tattttgatt attaatttct aagttgatag accacttttt tatttttcta
    15961 atacgctctt tagaagttta tgtattttca aaaccctaaa tcacttattg atttcataca
    16021 cttatcatgc attacatagg gagaagatat aaagaaggta tgtgttagtt cactactgtt
    16081 ttcttattga aggacgctaa aaattaaata ggttattaat caggttttgt agatttataa
    16141 aaaaaggact tagattatac atttatgtaa ccaagtttat tttacccctg tatcaaaggc
    16201 agtacatgct ctaattgaag tcctgtgtag gagcaaaact cttcaatagc caccaattgg
    16261 tgtggttctt tgttgaaatg ctttttaact tcaattagca gtaatcgtgc gtgtcgataa
    16321 cttttgccag taattctttg aacgtctttg gggtatatgc aaactctttt tagttccagt
    16381 ttcatatcct ttttatcagt cgggattgta ttcttgtttt aactgatggt cttttataaa
    16441 acaaatatac gctatttttc ttatttattc aaaaaagtaa tgggacaaaa tagtacctaa
    16501 tgggctaaaa tggtccctcc taaacactag tgtttttttt cttgattaaa ccctctaatc
    16561 tttgctttat aattcacaat tcggatgcat caaattagga ttataaggtt taaaagagtg
    16621 tacctgaaca ttctaagttt cattaaaaaa attattatca tggccagaca aaaaggcatc
    16681 attaaaataa aaggtactct tggagatatc accttttata aatcacagga tggttatctt
    16741 gtgaaagaga aaagcggggt tgatggaaag cgaatagcaa ccgaccctgc ttttcaaaga
    16801 actcgtgaaa acggatcgga atttggtagg gctggaaaag ctggaaaagc acttcgaatg
    16861 gcatttagac ctatactgat taagtgtgct gataaccgaa tggttggcag acttactcag
    16921 gcgatgatta aagtgcttca agcagacgcg gttagtgaac gtggattgcg caatgtaatc
    16981 gacggagaga ctgagttgtt ggttggattt gaatttaata ttcgtggaaa attaagcaac
    17041 agtttgtatg ctccatatgc tgcacagatt gatcgtgtaa caggggaact tactat
//
LOCUS       DAOOBH010000041        16575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_4554_length_16575_cov_5.320157, whole genome shotgun sequence.
ACCESSION   DAOOBH010000041 DAOOBH010000000
VERSION     DAOOBH010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 16575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16575
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4554_length_16575_cov_5.320157"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5748)
                     /locus_tag="PK218_05925"
     CDS             complement(<1..5748)
                     /locus_tag="PK218_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HPW98078.1"
                     /translation="MKTNYLKIKKISIQISILLISVLSFSQTVIKKQGFESSAADNLG
                     YSVSSGNVSVSSTTSMSGSKSLRFGTGTTNAIFDNVSLIGYSNVEVSIAFAATGVDND
                     EDLFVDFSYNNGSTYPDSIKLVDGNNGSGGQDLDFGTGDASPGQSSNPYTFTLPTGVS
                     SVKIRVRSISLDSNEYYYIDDIVIKEIPSPEIDITGDSISIANGDTTPNIFDWTDFGS
                     VDISSGTIIKTFTINNIGNATLTIANPTITGANASDFTVSTNISGTSINGGFYRIFRI
                     TFNPSAIGIRTAIINIVNNDSDENPYTFTIQGTGTNAEIDVLGNGISITDGSTSASVS
                     NNTDFGVTDITSGTITKIFTIKNYGTSPLLISNPTITGAHASEFTISSPPSTLSINPG
                     GSTNFSVTFDPAAISVRSAVINIANNDANENPYDFVIRGTGTNAEIDITGNSVSIANG
                     DTTPSLTDATDFGSLYLTGASQSNSFTIKNMGTTTLSISNPVISGPNASDFTVTVNPA
                     TTTVNGGTTKFFTIKFVPSAGGIRTAVVTLNNSDYDENPYVFTIQGYGVNPEINITGN
                     GVSIVNYDTTPTSADGTAYGNVLLPSGTSSKTYVIQNLGTSPLTISNPVISGINPSDF
                     TITSYPSTLSIPASGSTSFTVRFSTSVLGQRNAVITIDNNDYNENPYEFYINGYGDKD
                     TDGDGISDYEDEDDDNDGITDAIECKTCVTDPFMNGGFESPVIAASSYAILPTANVPG
                     WQTSAENFIEIWSSGFSSGSGGPVPAAVGNQFAELNANVPGILYQTFCLNGAGGTINW
                     SIKHRGRGGVDQAFVKFGSSLGNAIASTPIVTMVDGNTSWATYSGLYVIPVGQTQIVL
                     TFQAGYTGSGSQSVGNFIDDVQVVINQKCVDTDGDGIADIADVDDENDGIPDIEEAGF
                     KAYSNNKATFDMSNPALWKDTNNNGLNDYIEAMISAGTYIIPDNDNDLVPNHLDYDSD
                     NDSVFDVDEASLLNGDGDINGDGVGDGLDSDGDGLLDLFDNSPTFGTIGRTYAQDTDG
                     NGTPDFLQLKSDGIVFDISKTLYANLDADNDGIIDGFGDLDRDGIKDTFDTNNTIKGS
                     PRDLNRKLYLDFDGRNDYAEDSQILSGLSNATLMAWVDLGNGFNSTGYVVGQNKFYIR
                     VNSSRKIQVFVNGSNLQYNTPLDKNRWYHIAAVLGNGELKIYLNGTEVASKVYTSSIA
                     SDTTLLTLGKNPSALNSFFEGKIDEVRVFNEALTEKQLQRIVHQEIENNSGEIRGTVI
                     PKNIGSLPYNSLLRYYRMDTFKDDIIDDLTTPTVDLITGMKMYNHKVINYQQAPMPFV
                     TLRDGTFATAVNDPSKDIKGMDVIDNDATIIQVNHNITETGNNTDIGMIINAGASVNV
                     TNDTKMQNEWYLKMDGKIDLVGKSQLIQTEESELVATSAGSLERDQQGQSNRFNYNYW
                     SSPVSSINNTTINHGYTVAGVMKDGTDVNNVRNLQWTTGTNSSATSPITLSSYWIFKF
                     QNVGNNYYNWVSVGKDGTLLPGQGYTLKGSNAATPTQNYTYVGKPNNGTITSPVGANN
                     LMLAGNPYPSAIDADKFIEDNTASITGTLYFWEHYSTNASHNTVAYQGGYAAYNRTGG
                     TAPVAPSIISGLGSSSKTPKRFIPVGQGFFVTGSATGGTITFNNSQRLFVKEDNVASF
                     TMFRNAAESLTTATNNSEDTYTTEQFMKFRLGYNSANNYHRQILLGFMNEHATAGIDP
                     GYDALNFETLSNDMYFIKGTNKLNICGEGVFNIDNTYPLGVKNAVAGNVTFVLDNLEN
                     VDNNQQVYIFDNVTNTYNSIKGQNFEVTLPAGVTDNRFTLTFKNGTPDLGTSENELTN
                     GILVTNSQVANTINIKNQLMEATAKSVVLFNILGQQITSWDVENLDQSNIVLPTNGIS
                     TGAYIIGWKNNIALVK"
     gene            complement(6010..6083)
                     /locus_tag="PK218_05930"
     tRNA            complement(6010..6083)
                     /locus_tag="PK218_05930"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6047..6049),aa:Met,seq:cat)
     gene            6216..6608
                     /locus_tag="PK218_05935"
     CDS             6216..6608
                     /locus_tag="PK218_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HPW98079.1"
                     /translation="MDTKIRYELEFPINSSPQLLYQYISNPSGLQEWFADKVNSRGEF
                     FTFIWDDVEEFARLASKKSGEKIKFKWIDDNKKDTEFYFELRILEDEITKDVSLMVVD
                     FALEDEIEEAKLLWENQVSDLKHVIGSS"
     gene            6666..7496
                     /locus_tag="PK218_05940"
     CDS             6666..7496
                     /locus_tag="PK218_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPW98080.1"
                     /translation="MINFNGSIQNSDFNVSITNRSFLFGDGIFETMKIVDGKILFFED
                     HYFRLMASMRIVRMEIPMNFTLEFIENQILDLVSQLAILEAARVRFTVFRNDGGFYSP
                     VTNAVSYIIQANAIESKYYSFVNQKFEVDLYKDNLISKQILSTLKSTSKIVQVTASVF
                     AKENELDSCIMLNDSKNVVETISGNIFMLMDTVLITPPISEGCLNGIMRKQILGLAKF
                     FPEIEFKEAIISPFDLQKADELFLTNVIMGIQPISKYRKKEFQNIFTKKLLEKLNQTI
                     "
     gene            complement(7498..8058)
                     /locus_tag="PK218_05945"
     CDS             complement(7498..8058)
                     /locus_tag="PK218_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HPW98081.1"
                     /translation="MISEKLNKGSLLIAEPSIINDLSFNRSVILLADHNVEGSVGFIM
                     NKPLDYSIQDLLPEVNANFTVYNGGPVEQDNLYFIHNIPDLIPNSIEISNGIFWGGDF
                     ETTKNLINNNAIKKDNIRFFLGYSGWESNQLENEMLANSWIAIKNDYGNEILKKSVNE
                     FWKEKIIELGGDYLIWSNAPENPILN"
     gene            complement(8180..8452)
                     /locus_tag="PK218_05950"
     CDS             complement(8180..8452)
                     /locus_tag="PK218_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPW98082.1"
                     /translation="MNKSELIDAIAADAGITKAAAKLALESFLGNVGSTLKKGGRVSL
                     VGFGSWSVSKRAARDGRNPQTGKTIKIAAKNVVKFKAGAELDGSVN"
     gene            complement(8619..9566)
                     /gene="fmt"
                     /locus_tag="PK218_05955"
     CDS             complement(8619..9566)
                     /gene="fmt"
                     /locus_tag="PK218_05955"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPW98083.1"
                     /translation="MEKLRIVFMGTPEFAVGILDTIIKNNYDVVGIVTAADKPAGRGQ
                     KIKYSAVKDYALENNLTLLQPTNLKDESFLAELKRLNANLQVVVAFRMLPEVIWKMPK
                     LGTFNLHASLLPNYRGAAPINWAIINGELKTGVTTFYIDEKIDTGAMILSKEIEITQN
                     ENAGELHDRLMNLGSKAVVKTLNLIENGKASTTIQKDNPEIKTAYKLNKENCKIDWNK
                     SGKEIHNLIRGLSPYPCAWCFFVDKDNEWNVKIYDANFIEEAHDLETGLLLSSKKELK
                     VVVKDGFILLNELQFPGKKKMTTTELLNGLQFSALAQVK"
     gene            complement(9567..11465)
                     /locus_tag="PK218_05960"
     CDS             complement(9567..11465)
                     /locus_tag="PK218_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HPW98084.1"
                     /translation="MSKALEILKLYWKHESFRSLQEDIINSVVEEQDTFALMPTGGGK
                     SICFQIPALMKDGLCLVISPLVALMKDQVQNLQNKGIKAIALTGGIHENEIIELLDNC
                     QYGNYKFLYLSPERLQNDWILERIKNLPINLIAIDEAHCISQWGNDFRPAYLKINQLK
                     EYFPKVPYLALTATATERVQKDIIEKLELKDCKVFTKSFARENLAYMVFVVEDKLFLV
                     EQILRKNPQSSIIYVTNRKSCIDVSNQLEKMGFTATYYHGGLLAKDKENHMKLWMDEK
                     VQVMVATNAFGMGIDKDNVKTVIHLHLPSNLENYYQEAGRAGRNGDKSFAVLLVNPSD
                     LLHAESQFIFVLPDKNFLNLVYNKLCNFLRIGYGEGIEEQFNFNFNLFCLQYELPILK
                     TYNSLQFLDNQGIITFNQEFSEKISLQFTIPSKEVIRYISLNLNDEEIMLTILRTYPG
                     IYDMHTVFNTQLIAKKSNSSETKVLKVLEKLKEKNIIELLSKKNDTSITFNEVREDER
                     TINRVTKSLEKINTLKIAQLKSVVAYASNTKECKNKTLLAYFGETIEKDCGICSFCIS
                     KKKSPKKNDKLPSKIIELLKSNDFNSREIQKSTKANEDDVIFALQTLMEHNRIILKST
                     NLYSLKKN"
     gene            complement(11537..12073)
                     /locus_tag="PK218_05965"
     CDS             complement(11537..12073)
                     /locus_tag="PK218_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW98085.1"
                     /translation="MEKEIIVITGGPGTGKTTIIDGLIEQGYSCFPEISRQITLEAKK
                     QGIEQLFLEKPLLFSELLLEGRKKQFDKAVQDANEIVFLDRGVPDILAYMHYIGDSYP
                     SFFDKACNDIRYSKIFLLPPWENIYVSDEARYENFEQAKLIYDHLKETYKKYDYQLIE
                     VPTGTIEERIQFILSELD"
     gene            12224..13753
                     /gene="guaA"
                     /locus_tag="PK218_05970"
     CDS             12224..13753
                     /gene="guaA"
                     /locus_tag="PK218_05970"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPW98086.1"
                     /translation="MQHNVLILDFGSQYTQLIARRVRELNIFCEIFPYNNIPTDLSSY
                     KAVILGGSPYSVRSEDALQIDMSNFRGKMPLLAVCYGAQYLAHFSGGEVAASTIREYG
                     RANLTYIKENEIFFEDVNLNSQVWMSHSDSIKHLPNNGIKLASTKDVENAAYRIDGET
                     TYAIQFHPEVYHSTDGKQILENFLVKIAQVPQNFTPNAFVEDCVKELQEKVGNDKVVL
                     GLSGGVDSTVAAVLLHKAIGKNLYCIFVNNGLLRKNEFENVLEQYKGMGLNVKGVDAS
                     KRFLSALADIEEPELKRKAIGRVFIEVFDDEAHQIEDVKWLAQGTIYPDVIESVSVKG
                     PSATIKSHHNVGGLPDFMKLQIVEPLRMLFKDEVRRVGKTLGIDAELLGRHPFPGPGL
                     SIRILGAITPEKVQILQEVDAVFINGLKEHGLYDKVWQAGAILLPVNSVGVMGDERTY
                     EKVVALRAVESTDGMTADWVHLPYDFLMKISNEIINKVKGVNRVVYDISSKPPATIEW
                     E"
     gene            13853..15871
                     /locus_tag="PK218_05975"
     CDS             13853..15871
                     /locus_tag="PK218_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPW98087.1"
                     /translation="MKNNIAFLLLVFTSFVFAKQDKYLKHTVSQGETITQIAQKYKVT
                     PFDIYKLNPDSQKGIQLNSVLLIPSNGNSVITSQVKTQVLPTRKATTHLVLPKETLFS
                     LSRMYNVSVDEIKNANVELLKNGLKIGQNVVIPASDGTSAFSENSVTSAPKEVVKSVS
                     KPESKQESKQQVTSNSTEKFHVIAPKETKFGISKMYGITIQELERLNPEIVSSFPIGL
                     RLVISGNPATQAVYDTTKSTSTTGNSSGFSTKRYLEEYVVRPNETVYSISRDYGISEQ
                     ELLYLNPELKKALKLGMILRVPKGQKKEVFIKEKLDLVKTIKADKKKKLVMLLPFNVS
                     KIQSDTVNSTEARLKRDKFLNLTLDFYSGALMAIDSAKTLGLNIDVKIYDSQETKNTS
                     SLVELYTNHSFENADAILGPFYQSNVEKLAEWLINANTPIISPLSKEYGKSYPNLYQS
                     LPSEDKMRNTMFDYMRSKQGNIVAVIDSKKQSIYDYFSKNQPDVKIIGLSEKGTLVAD
                     SLNVRLSKTRPNYVVLVTEKTSMILNTTNIMQKLKQEYQMQLAILEYNETLDFEEVKL
                     ANLTNLNMMYPSITKPNDSAMNTGFENSYKKQNNVFPNQFAVRGFDVTFDTLLRLSQE
                     KTFEETIQSASTEYVENKFDYDQSEAGYVNNGVFVLYYDTDLTIKEAK"
     gene            15871..16275
                     /locus_tag="PK218_05980"
     CDS             15871..16275
                     /locus_tag="PK218_05980"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPW98088.1"
                     /translation="MPTSKITYQGELRTICIHLQSGTEILTDAPTDNHGKGEAFSPTD
                     LVATALGSCMVSIMAIKSKDLGVDLKDSTIEVTKIMQSEPRKIAEIIVVLNMSISTDD
                     KTKIILERAANTCPVLLSLHPDIKKKVTFNWK"
     gene            16279..16506
                     /locus_tag="PK218_05985"
     CDS             16279..16506
                     /locus_tag="PK218_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HPW98089.1"
                     /translation="MNSQQEQLIKLAQTKMPYGKYEGKYLIDLPEYYIVWLNNNRLPK
                     GKLGEQLKLIYELKLNGLEEIVRTIRNKYSK"
BASE COUNT         5480 a         2658 c         2645 g         5792 t
ORIGIN      
        1 cttgaccaaa gcaatattgt tcttccaacc aatgatataa gcacctgtgc tgataccatt
       61 ggttggaaga acaatattgc tttggtcaag gttttcaaca tcccaagaag tgatttgttg
      121 accaagtatg ttgaacaata ctacactttt ggcagttgct tccattaatt gatttttaat
      181 gttaatcgtg ttggctactt gcgaatttgt aaccagtatg ccatttgtca attcattttc
      241 actcgttccc agatcaggtg ttccgttttt gaacgttaat gtgaagcgat tatcagttac
      301 tcctgcaggt agcgtaacct cgaagttttg tcctttaata ctgttgtagg tattggtaac
      361 attatcaaag atataaactt gttggttgtt atctacattt tctaaattat caagtacaaa
      421 agttacatta ccagcaacag catttttaac acccaacgga tatgtattgt ctatattgaa
      481 gactccttcg ccacaaatgt tcaacttatt tgttcctttt ataaaataca tatcattgct
      541 taaagtttca aagtttagcg catcatatcc tgggtcaatt ccagcggtag catgctcgtt
      601 cataaatcct aacaatattt gtctgtggta attattggca gaattgtatc ccaatctgaa
      661 tttcataaat tgttcagtag tataagtgtc ttcagaattg tttgtagcag tagttaaact
      721 ttcggctgca tttctaaaca ttgtaaatga agctacattg tcttctttta caaataatcg
      781 ttgactatta ttgaaagtta ttgttccgcc agtagccgag cctgtaacaa aaaatccttg
      841 tccgactggg ataaatctct taggagtttt gctacttgaa cctaatccac taataatgga
      901 aggagcaact ggagcagttc caccagttct attgtaggct gcatatccac cttgataagc
      961 cacggtgttg tgagatgcgt tagtgctata gtgttcccag aaatataatg ttccagtgat
     1021 tgaagcagta ttgtcttcta taaatttatc ggcatcaata gctgatggat aagggttacc
     1081 tgctagcatt agattatttg ctccaactgg acttgtgatg gttccattat ttggttttcc
     1141 aacataagta taattttgag taggagttgc agcattacta ccttttaaag tataaccttg
     1201 accaggaaga agcgtgccat cttttccaac agaaacccaa ttgtaataat tgtttcctac
     1261 gttttggaat ttaaatatcc agtagctgct cagagtaatt ggcgaagtag cactactatt
     1321 agttcctgtt gtccattgta agtttctaac gttgtttaca tctgtaccgt ctttcatcac
     1381 accagcaaca gtgtaaccat gattaatagt agtgttattt atagagctta caggagaaga
     1441 ccagtagtta tagttaaacc tatttgattg accttgttgg tctctttcaa gagaaccagc
     1501 actagtagca accaattctg actcttctgt ttgtattaac tgtgatttac caactaagtc
     1561 tatttttcca tccattttta aataccattc gttttgcatt ttagtgtcat tggttacatt
     1621 aacacttgca cctgcattga taatcatacc tatatcagtg ttattaccag tttcagtaat
     1681 gttgtgattt acttgtataa ttgtggcatc attatcaata acatccattc ctttaatatc
     1741 ttttgaaggg tcatttacag cagtagcaaa tgttccgtct ctaagcgtta caaaaggcat
     1801 tggtgcttgt tggtaattga taactttgtg attgtacatt ttcataccag taattaaatc
     1861 tacagtaggt gttgtcaaat catcgataat atcatcttta aatgtgtcca ttctatagta
     1921 acgtaacaaa gaattataag gtaatgatcc aatgtttttt gggattacag ttcctctaat
     1981 ttcaccacta ttattttcaa tttcttggtg tacaattcgt tgaagttgtt tttcggtcag
     2041 tgcttcattg aatactctta cttcatcaat ttttccttcg aagaaactat ttaaagcaga
     2101 aggattttta cctaatgtta ataatgttgt atcacttgct atgctagaag tataaacttt
     2161 tgaagcaact tcagttccgt ttaaatagat ttttaattct ccattgccta gtactgctgc
     2221 aatatgatac catctgtttt tgtctaaagg ggtattgtac tgtaaattac taccgtttac
     2281 aaatacttgg atttttcgac tagaattgac tctaatataa aatttatttt gaccaacaac
     2341 atatcctgtt gagttgaatc catttcctaa atcaacccaa gccattaaag ttgcattgga
     2401 aagcccactc aaaatttgag aatcttcagc ataatcattt ctgccatcaa aatctaaata
     2461 taattttctg tttaaatctc ttggagagcc tttaatagta ttgtttgtgt caaatgtatc
     2521 tttaattcca tctctgtcta agtcaccaaa accatctata attccatcat tatctgcatc
     2581 taaattagca tataatgttt ttgaaatgtc aaatacaatt ccatcacttt ttaattgtaa
     2641 aaagtcaggt gttccatttc catcagtatc ttgtgcataa gttctgccaa tggttccaaa
     2701 agtaggtgaa ttatcaaata agtctaataa tccatctcca tcagaatcta atccatcacc
     2761 aacaccgtca ccattaatat cgccatctcc gtttaaaaga ctagcttcat caacgtcaaa
     2821 cacactatcg ttatcagagt cataatctaa atgatttggc acaaggtcat tatcattgtc
     2881 aggaatgata taggttccag cagaaatcat ggcttcaata taatcattta aaccattatt
     2941 gtttgtatct ttccataaag ctgggttact catgtcaaaa gttgctttgt tattactgta
     3001 ggctttgaat ccagcttctt caatatctgg aataccatca ttttcatcat ccacatcagc
     3061 aatgtcagca attccatcac catctgtatc aacacatttt tgattgataa ctacttgtac
     3121 atcatctata aagtttccta ctgattggct tccggaacca gtgtatcccg cttgaaaagt
     3181 aagcacgatt tgggtttgac ctactggaat aacatataat cccgaatagg ttgcccatga
     3241 agtgtttcca tcaaccatgg ttacaatagg agtagaagca atggcatttc ctaatgaaga
     3301 accaaacttt acgaaagctt gatcaactcc accacgacct ctgtgtttga ttgaccagtt
     3361 tattgttcca ccagcaccat ttaaacaaaa cgtttgatat aatatacctg gtacattggc
     3421 atttaattct gcaaattgat ttccaacggc tgcaggaact ggtccaccac taccactact
     3481 aaatcctgaa ctccaaattt ctataaaatt ttctgcacta gtttgccatc caggaacatt
     3541 tgctgttgga agaatagcat aacttgaagc tgcaatgact gggctttcaa aaccaccatt
     3601 cataaatgga tcggtaacac atgttttaca ctcgattgca tcagtaattc catcattgtc
     3661 atcatcttca tcttcataat cggatatacc atcaccatca gtgtctttat caccatatcc
     3721 gtttatataa aattcataag ggttttcatt ataatcatta ttatcaattg ttatgacagc
     3781 atttctttgt cctaaaacag acgttgaaaa acgcacagta aaagaggtgc ttccacttgc
     3841 aggaattgaa agagtggaag gataagatgt tatggtaaaa tcagaaggat ttattccaga
     3901 gataacagga tttgaaattg ttaacggtga tgttccaaga ttttgaatta catatgtttt
     3961 tgaagaagtt ccactaggaa ggagaacatt tccataagca gtaccatctg cacttgttgg
     4021 agtggtgtca taatttacaa tggaaacccc attgcctgtt atattaattt ctggattaac
     4081 cccatagcct tgaatagtaa aaacatatgg gttttcatca taatcactat tgtttaaagt
     4141 aacaacggct gttcttattc cacccgcact tggaacaaat ttaatagtaa aaaattttgt
     4201 tgttccacca ttcacagttg tggtcgctgg gtttacagtt actgtaaagt ctgaagcatt
     4261 tggaccacta attacagggt ttgaaatgct caaagtagtt gtgcccatgt tttttatagt
     4321 aaatgagttt gattgtgagg caccagttaa ataaagtgaa ccaaaatctg tagcatctgt
     4381 taaagaaggt gtagtatcac catttgcaat tgaaactgaa tttccagtaa tgtcaatttc
     4441 tgcatttgtt ccagttcctc ttatgacgaa atcataaggg ttttcatttg catcattatt
     4501 agctatatta ataacagcac ttctaacact tattgcagct ggatcaaatg ttactgagaa
     4561 atttgtagaa cctccagggt ttatggataa tgtagaagga ggagatgaaa tagtaaattc
     4621 tgatgcgtgt gcacctgtaa tggtagggtt tgatattagt aaaggagaag ttccataatt
     4681 ttttatagta aatattttgg taattgtacc cgaagttatg tctgttactc caaaatcagt
     4741 attgttggaa actgatgcgc ttgtagaacc atcagtaatt gaaattccat tgcctaaaac
     4801 atcaatctca gcatttgtac ctgttccttg aattgtaaat gtatatggat tttcatcact
     4861 gtcattatta actatgttta ttattgctgt tcttattcct attgcgctag ggttaaaagt
     4921 tatcctaaat attctgtaaa aaccaccatt tatagatgtt ccactaatgt ttgtgctaac
     4981 ggtaaaatct gaagcattgg cacctgtaat tgttggattt gcgatagtta atgtagcatt
     5041 tccaatattg ttaatcgtaa atgttttaat tattgttccc gatgaaatat ccacagatcc
     5101 aaagtcagtc cagtcaaaaa tatttggggt agtatctcca ttggctattg atattgaatc
     5161 acctgtaata tctatttctg gagatggaat ttctttgata actatatcat caatataata
     5221 atattcatta ctatctagac ttattgatct aactcttatt ttaacactgg aaactcctgt
     5281 tggaagagta aatgtatatg gatttgatga ttgacctgga gaagcatcac cagttccaaa
     5341 atctaagtct tgtcctccac ttccattatt tccatcaaca agtttaattg aatcaggata
     5401 tgtactaccg ttattatatg aaaaatccac aaataaatct tcatcattat ctactccagt
     5461 cgcagcaaaa gcaattgata cttctacatt actataacca attaagctta cattatcaaa
     5521 aattgcatta gtcgttcctg ttccaaatct taaagatttt gatccactca ttgatgttgt
     5581 gctgcttact gaaacatttc cagatgaaac ggaatatcct agattatcag ctgcgctact
     5641 ttcaaaacct tgttttttta ttaccgtttg cgaaaaactt aatacagaga ttaataaaat
     5701 gctaatctgt attgagattt ttttgatttt taaataattt gttttcatgt tttttacaat
     5761 ttattttttt tgacacacca aaactagatt gttttgatta tataatcgat aaaaagcata
     5821 aaataatcga tgaaatacat gttttgttat ttaaaactaa gtatagtatt acatgttcaa
     5881 aatttaaaat aatccattaa atgcaaatta tgccgatgaa atgcactatt ttaattttat
     5941 tgtaagtttg ggagtattta tttttggaaa taaaaaaacg ccactttgga ataagtgacg
     6001 tttcaaatgt agcgagaggg agatttgaac tcccgacctc agggttatga atcctgcgct
     6061 ctaaccaact gagctacctc gccaattgtt agcgggtgca aatataaaat tattattaat
     6121 ggttgcaaac attaattaag tatttttttt atttttaaaa atgctttttt tttgggggat
     6181 tattctatat atttccaaaa attttaagat taatcatgga cactaagata agatatgagt
     6241 tagaatttcc aataaattcc tcaccgcagt tattgtatca atatatttct aatccttctg
     6301 gtttacaaga atggtttgca gataaagtaa attctagagg tgagttcttt acttttatat
     6361 gggatgatgt agaagagttt gcacgattgg catccaaaaa atctggagaa aaaataaaat
     6421 ttaaatggat tgatgacaat aaaaaagata cagagtttta ttttgaactt agaattcttg
     6481 aagatgaaat tacaaaagat gtttctttaa tggtggttga ttttgcttta gaagatgaaa
     6541 tagaagaagc aaaactttta tgggaaaatc aagtttctga cttaaaacat gtcataggtt
     6601 ctagttaaaa tagtataaaa acttatattt gcaccgtttg gtattaatac taaacggttt
     6661 tttttatgat aaattttaac ggctctatac agaattcaga ttttaacgtg tcaatcacca
     6721 atcgttcttt tttatttggt gatgggattt ttgaaaccat gaaaatagtg gatggaaaaa
     6781 ttcttttttt tgaagatcat tattttagac taatggcttc gatgagaatt gtaagaatgg
     6841 aaatcccaat gaattttaca ttagagttta tagaaaatca aattttagat ttagtttccc
     6901 aacttgcaat tttagaagct gctcgtgtaa gatttacagt ttttagaaat gacggtggct
     6961 tctattcgcc agtaactaac gctgtctctt acatcattca agcaaatgct attgaaagta
     7021 aatattattc ttttgtaaat caaaaatttg aagttgattt gtataaagac aatttaattt
     7081 caaaacaaat tctttccact ttaaaatcaa ccagcaaaat agtacaggta accgcaagtg
     7141 tttttgccaa agaaaatgag ttagattctt gcataatgtt gaatgattca aaaaatgtag
     7201 tggaaacaat ttcgggaaat atttttatgt taatggatac tgttttaata acgccgccaa
     7261 tttctgaagg ctgtttgaat gggataatgc gtaaacagat tcttggattg gcaaagtttt
     7321 ttcctgaaat tgaattcaaa gaagcaatta tttcaccatt tgatttacaa aaagccgacg
     7381 aattattttt aacaaatgta ataatgggaa ttcagcctat atcaaaatat agaaaaaaag
     7441 aatttcaaaa tatattcact aaaaaactgt tagaaaaatt aaaccaaacg atttaaatta
     7501 attaagtatt ggattttcgg gagcgtttga ccatattaga taatctccac caagttcaat
     7561 gattttttct ttccaaaatt cgttaactga tttttttaag atttcatttc cataatcatt
     7621 tttgattgca atccaagaat tggccaacat ttcattttct aactgattgc tttcccatcc
     7681 tgaatatcct agaaaaaagc gtatattgtc tttttttata gcgttgttat taattaaatt
     7741 cttggtagtc tcaaaatcac caccccaaaa aatgccatta gaaatttcaa tactattggg
     7801 gattaaatct ggaatattat gaatgaaata caaattatct tgttcgacag gaccgccatt
     7861 ataaactgta aaatttgcat ttacttcggg aagtaaatct tgaatactat aatctaaagg
     7921 tttattcata ataaaaccaa ccgaaccttc tacgttatgg tctgccaaaa gaataacaga
     7981 acgattaaaa gataagtcgt taatgattga aggttctgca attaataaac ttcctttatt
     8041 taatttttct gaaatcatat tcataaaatt tttaataaat ctaataaaaa gtatttaaaa
     8101 aaactaattt gaatcaataa atcaaaaaaa gccacatcaa ttgatgtggc ttttaagagt
     8161 atttttgtta agctacttat tagtttaccg aaccgtctaa ttcagcacca gctttgaatt
     8221 ttactacatt ttttgcagca attttaatag tttttccagt ttgaggattt ctaccatctc
     8281 tagccgctct tttagataca gaccaagatc cgaaacctac taaagaaact cttccacctt
     8341 ttttcaaagt gctacctaca tttcctaaaa atgattctaa tgctaatttt gcagcagctt
     8401 ttgtgattcc agcgtcagct gcaattgcat cgattaattc tgatttgttc ataataatag
     8461 ttattaattg ttggttaaac attaagttat aataaacaaa attaatagga attgcttacc
     8521 acgcaagagt tttgatgttt ttttagcttt tttgttgata acttaaaaca tttgttgata
     8581 aaccaatgct tttcatcgat ttttataatt tggatgccct attttacttg tgctaaagct
     8641 gaaaactgaa gtccatttaa aagttcagta gtagtcattt ttttctttcc aggaaattgt
     8701 aattcattta gcaatataaa tccatctttg acaacaactt ttaattcttt tttgcttgaa
     8761 aggagtaagc ctgtttccaa atcatgagct tcttcaataa agttagcatc ataaattttt
     8821 acattccatt cgttgtcttt atccacaaaa aaacaccaag cacatggata gggtgacaag
     8881 cctcgaatta agttgtgtat ttcttttccg cttttattcc aatcaatttt gcaattttct
     8941 ttgttgagtt tgtatgcagt ttttatttct gggttatcct tttgaatagt tgtgcttgct
     9001 tttccattct caatcaaatt tagtgttttt acaacggctt tgcttcctaa attcattaat
     9061 cgatcatgca attcgcctgc attttcattc tgagtaattt ctatttcttt ggataaaatc
     9121 atagcacctg tatcaatttt ttcatcaata tagaatgtag tcacgccagt ttttagttca
     9181 ccattaatta ttgcccaatt gattggagct gcaccacgat aatttggtag taaagaggca
     9241 tggagattga atgtacctaa tttaggcatt ttccaaatta cttcgggaag cattctaaag
     9301 gcaacaacaa cttgaagatt tgcatttaac ctttttaatt ccgccaagaa actttcgtct
     9361 tttagattcg ttggttgcaa aagagtcaaa ttgttctcta aagcataatc tttaacagct
     9421 gaatatttaa tcttttgtcc tcgaccagcg ggtttatctg ctgcagtaac tattccaact
     9481 acatcataat tgtttttaat gatggtgtct aaaattccaa cagcaaattc gggtgttccc
     9541 ataaaaacga tgcgtagctt ttccatttag ttctttttta gcgaatataa attagttgac
     9601 tttaggatga ttctgttgtg ttccattaaa gtttgaagtg caaaaataac atcatcttca
     9661 ttagctttag tgctcttttg aatttctctt gaattaaaat cattgctttt taataattca
     9721 ataatttttg acggtaattt gtcatttttt tttggagatt tttttttaga aatacaaaac
     9781 gaacaaatgc cacaatcttt ttcgattgtt tcaccaaaat aggcaagtag cgttttattt
     9841 ttacattctt ttgtattgga agcataagca actaccgatt ttagttgtgc tatttttaaa
     9901 gtatttattt tttctaaaga tttagtaact cgattgatgg ttctttcatc ttctcgaact
     9961 tcgttgaaag tgattgatgt atcatttttt ttagaaagta attcgatgat gtttttctct
    10021 tttaattttt ctaaaacttt caaaactttc gtttctgatg aatttgattt tttggcaatt
    10081 aattgcgtgt taaaaacagt atgcatatca taaattccag gatacgttct caaaatggtt
    10141 aacatgattt cctcatcgtt taaatttagg cttatataac gaataacttc tttggaagga
    10201 atagtaaatt gtagtgatat tttttctgaa aattcttgat taaaagtaat tatcccttga
    10261 ttgtctagga attgtagtga gttataggtt tttagaatag gcaattcata ttgaaggcaa
    10321 aacaggttaa aattaaaatt aaattgttct tcaattcctt cgccataacc tattctaagg
    10381 aaattgcaga gtttgttata aactaaattc aaaaagtttt tgtcgggtaa aacgaagata
    10441 aactgcgatt cagcatgaag taaatcggat ggatttacca aaaggactgc aaaagatttg
    10501 tcaccatttc ttccagcacg accagcttct tgataataat tttctaaatt actcggaaga
    10561 tgcagatgaa taactgtttt tacgttgtct ttgtcaattc ccatcccaaa agcatttgtg
    10621 gccaccatca cttgaacttt ttcatccatc caaagtttca tgtgattttc tttgtctttt
    10681 gctagtaatc ctccgtgata ataagtggcg gtaaatccca ttttttccag ttgatttgat
    10741 acatcaatac acgattttct attggtaaca tatataatag aagattgcgg attttttctc
    10801 agaatttgtt ctaccaaaaa cagtttgtct tcgactacaa aaaccatata agcaaggttt
    10861 tctctagcaa aagattttgt gaaaacctta caatctttaa gttccaattt ttcaataata
    10921 tctttttgaa cgcgttctgt agcagtagct gttaatgcta aataaggtac tttaggaaaa
    10981 tattctttta attggttaat ctttaaataa gcaggacgaa aatcatttcc ccattgcgaa
    11041 atacaatgtg cttcatctat agcgattaga tttattggaa gattttttat ccgttctaaa
    11101 atccaatcat tttgcaaccg ttcaggtgaa agatataaaa atttataatt accatattgg
    11161 caattgtcta gtaattcgat gatttcgttt tcatgaatac cacccgttaa tgcgatggct
    11221 tttatccctt tgttttgaag attctgaact tggtctttca tcaaagctac caatggtgaa
    11281 atgaccaaac acaatccatc tttcataagt gctggaattt gaaaacaaat tgattttccg
    11341 ccacctgttg gcattaaagc aaaagtatct tgttcttcaa ctaccgaatt gataatgtct
    11401 tcttgaagtg atctaaaaga ttcgtgtttc caataaagtt taagaatttc taatgctttt
    11461 gacatgtata aaagttgatt tcctttgaaa gtatcaaagc taatgaaatt tacaataaaa
    11521 aagaatgtac taggtattaa tccaattcac tcaaaataaa ttgaatacgt tcttctatag
    11581 ttccagttgg aacttcaatc aattgataat cgtatttttt ataagtttct tttaaatgat
    11641 cgtatatcaa tttggcttgt tcaaaatttt cataacgtgc ctcatcactc acatatatgt
    11701 tttcccaagg tggtaataaa aatattttcg agtatctaat atcgttgcaa gctttatcaa
    11761 aaaaagaggg ataactatcg ccaatgtaat gcatgtatgc caaaatatca ggaacaccac
    11821 ggtctaaaaa aacaatttcg tttgcgtcct gaacagcctt gtcaaattgt ttttttctac
    11881 cttctaacaa taattcacta aagagaagtg gtttttcaag aaataactgt tcaattcctt
    11941 gttttttggc ctcaagtgtt atttgtctag aaatttcggg aaaacatgag taaccttgtt
    12001 cgattaatcc atcgattatt gttgtttttc cagttcctgg tccgccagta attacaatta
    12061 tttctttctc caaaattttt gaaataagtg gcaaatttat tgaaagtatt taagaaacat
    12121 cgtttaaatc agatttttta attaataatt tggttttatt catttttttg caaaaactca
    12181 aaaaatcaat ttttaaattc aattgagaat agtacttttg cacatgcaac acaacgtact
    12241 tattttagat tttggctcgc aatatacaca actaattgca cgccgcgttc gcgaattaaa
    12301 catattctgc gaaatttttc cttacaacaa cattccaact gatttgtcgt catacaaagc
    12361 agtaattctt ggcggaagtc cttactctgt tcgctctgaa gatgctttgc aaattgatat
    12421 gtccaatttt cgtggaaaaa tgccattatt agcagtgtgc tacggtgcgc aatatttggc
    12481 tcatttttca ggaggtgaag tagctgcttc tactattaga gaatatggaa gagcaaattt
    12541 gacttatata aaagaaaacg aaattttctt tgaagatgtt aacctaaatt ctcaagtttg
    12601 gatgagtcat agtgactcca taaagcattt gccaaacaat ggtattaaac tagcgagtac
    12661 caaagatgtt gaaaatgctg cttatagaat tgatggcgaa actacttatg ctattcagtt
    12721 tcatccagaa gtatatcatt caacagatgg aaaacaaatt cttgaaaatt tcttagtgaa
    12781 aattgctcaa gttcctcaaa attttacgcc aaatgcgttt gtagaagatt gtgttaaaga
    12841 attacaagaa aaagttggaa acgacaaagt agttcttggg ctttctggcg gtgttgattc
    12901 gacagttgca gcggttttat tgcacaaagc aattgggaaa aatctctatt gcatatttgt
    12961 aaacaatggt ttgcttcgga aaaatgaatt cgaaaatgtt ttagaacagt ataaagggat
    13021 ggggttaaat gttaaaggag ttgatgcctc aaaacgtttt ctttctgctt tagccgatat
    13081 tgaagagcca gagttaaaac gaaaagctat aggtagagtt tttattgaag tttttgatga
    13141 cgaagcacat caaattgaag atgtaaaatg gttggcacaa ggaacaattt atcctgatgt
    13201 tattgagtct gtttcagtaa aaggaccaag tgccactatt aaatcacacc ataatgttgg
    13261 cggattacct gattttatga aacttcaaat tgtagaacca ctccgaatgt tatttaaaga
    13321 tgaagttcga agagttggaa aaacacttgg tattgatgca gagcttttag gaagacatcc
    13381 ttttccaggt ccaggtttgt cgattagaat tttaggtgcc atcactccag aaaaagtgca
    13441 aattttacaa gaagtcgatg ctgtttttat caacggtttg aaagaacatg gtttgtatga
    13501 taaagtatgg caagcgggcg ctattttact tccagtaaat agtgttggtg ttatgggtga
    13561 tgaacgtact tatgaaaaag ttgtagcgct tcgtgcggtt gaatctactg atggaatgac
    13621 agcagattgg gtacatttgc cttatgattt tttgatgaaa atttccaatg aaataattaa
    13681 taaagtcaaa ggtgttaata gagtagtgta tgatattagc tcaaaaccac cagccactat
    13741 agaatgggaa tagtttttgt taacagtaaa tagttaatga aaataaatac atttatcatt
    13801 gatgttaaaa agttttacta actttagacg ttaatttatt atgattgaga atatgaaaaa
    13861 caacatagct ttcttgcttt tagtgtttac tagttttgtt tttgcaaaac aagataaata
    13921 tttaaaacat actgttagtc aaggagaaac cattacacaa attgctcaaa agtataaagt
    13981 tactccattt gatatctata agttaaatcc tgattctcaa aaagggatac agttaaattc
    14041 ggtattgcta attccttcaa atggaaattc tgtaattact tctcaagtta aaactcaggt
    14101 tttgccaact cgaaaagcta caacgcattt agtgctgcca aaagaaacgc tttttagttt
    14161 gtcgagaatg tataatgttt cggttgatga aattaaaaat gcaaatgtag agttgttgaa
    14221 gaatggatta aagattggtc aaaatgtagt cattcctgct agtgacggaa cttctgcttt
    14281 ttcagaaaac agtgtaacat ctgctccaaa agaagtggtg aaatcagttt ctaaacccga
    14341 atcaaaacaa gagtcaaaac aacaagtaac atcaaattca acggaaaaat ttcatgtcat
    14401 cgcacctaaa gaaaccaaat ttgggatttc aaagatgtat ggcattacca ttcaagaatt
    14461 agaacgatta aatccagaaa tcgtaagtag ttttccaata ggtttgcgtt tggttatttc
    14521 aggaaatcct gctacacaag ccgtttatga tactacaaaa tcaacttcaa caacaggtaa
    14581 ttcttctggt ttttcgacca aaagatattt agaagaatat gttgttcgac caaatgaaac
    14641 ggtttatagt atttcaaggg attatggcat ttccgaacaa gaactacttt atttgaatcc
    14701 agaattaaaa aaagcattaa aacttgggat gattcttcgt gttccaaaag gtcaaaaaaa
    14761 ggaagttttt ataaaagaaa aattggattt ggtaaaaaca attaaagcag ataaaaagaa
    14821 aaaattggta atgttgttgc cgtttaatgt ttccaaaatt caaagtgata cagtaaattc
    14881 aaccgaagct agattaaaac gcgacaagtt tttaaaccta actttagatt tttattcagg
    14941 tgctttgatg gcaattgatt ctgctaaaac gttagggttg aatatagacg taaaaattta
    15001 tgattcgcaa gaaactaaaa acacctcaag tttggtagaa ctttatacga atcatagttt
    15061 tgaaaatgcg gatgcaatac ttggaccttt ttatcaatcc aatgttgaaa aattagcaga
    15121 atggttgatt aatgctaaca cgccaattat ttctccattg tcaaaagaat acggtaaaag
    15181 ttatccaaac ctttatcaat cgttgccatc cgaagacaaa atgcggaaca cgatgtttga
    15241 ttacatgcgc agcaaacaag gaaatattgt tgcagtaata gactcaaaga aacaaagtat
    15301 ttatgattac ttttctaaaa atcaaccgga tgtaaaaatt attggtttga gtgaaaaagg
    15361 cactttggta gctgatagtt tgaatgttcg tctttctaaa acaagaccaa attacgtggt
    15421 tttagttacc gaaaaaacct ctatgatatt gaacacaacc aatattatgc aaaagttaaa
    15481 acaagagtat caaatgcaat tggcaatatt agaatacaat gaaacacttg attttgaaga
    15541 agtaaaattg gccaatttga ccaatttaaa catgatgtat ccatcgatta caaaaccaaa
    15601 tgatagtgct atgaatactg gttttgaaaa cagttataaa aaacaaaaca atgtttttcc
    15661 taatcaattt gctgttagag gatttgatgt tacctttgat actttattgc gtttgtctca
    15721 agaaaaaaca tttgaagaaa ctattcaatc cgcatctacg gaatatgtag aaaacaaatt
    15781 tgattatgat caatccgaag cgggctatgt aaataatggt gttttcgttt tatattatga
    15841 tactgattta accattaaag aagcaaaata atgccaacct caaaaattac ttatcaagga
    15901 gaattaagaa caatttgtat tcatctacaa tcaggaacag aaattttaac tgatgctcca
    15961 acagacaatc acggtaaagg agaagccttt tcaccaacag atttagttgc cactgcttta
    16021 ggaagttgta tggtttccat tatggcaata aaatctaaag atttaggagt tgatttaaaa
    16081 gactcaacaa ttgaagtaac caaaattatg caatctgaac ctagaaaaat tgcagaaatt
    16141 attgtggttt tgaatatgtc aatttccaca gatgataaaa ccaaaatcat attagaaaga
    16201 gcggcaaaca cttgtccagt attattaagt ttgcatccag atattaaaaa gaaagtcact
    16261 tttaattgga aataactatt gaattctcaa caggaacaac tcattaaact cgctcaaacc
    16321 aaaatgcctt atggtaaata tgaaggcaaa tatctcattg acttacctga atattatata
    16381 gtttggttaa acaataatag actaccaaaa ggtaagcttg gcgaacaact caaacttatt
    16441 tacgaactca aattaaacgg tttagaagaa attgttcgaa cgattagaaa caaatattca
    16501 aagtaaaaac tccaagaaaa aacaatttca aaattgaacc aatttctcat tggcacattg
    16561 actaattaat cgtat
//
LOCUS       DAOOBH010000042        16257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_4665_length_16257_cov_7.362918, whole genome shotgun sequence.
ACCESSION   DAOOBH010000042 DAOOBH010000000
VERSION     DAOOBH010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 16257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16257
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4665_length_16257_cov_7.362918"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1592
                     /locus_tag="PK218_05990"
     CDS             204..1592
                     /locus_tag="PK218_05990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPW98090.1"
                     /translation="MDNIKQYVQENKERFINELIELLKIPSVSADSAYAHDVLATADA
                     VKESLKNAGCQHVEICETEGYPIVFGKHIIDENLPTVLVYGHYDVQPPDPIELWTSPP
                     FEPVIKATEIHPEGAIFARGACDDKGQMYMHVKAFEYMIKNNCLPCNVKFMIEGEEEV
                     GSKSLGTFVENNQEKLANDVILISDTGMISNQQPSITTGLRGLSYVEVEVTGPNRDLH
                     SGLYGGAVANPINILSKMIASLHDENNHITIPGFYDKVEELSAAERAEMAKAPFSIEN
                     YKKALDINDVFGETGYVTNERNSIRPTLDVNGIWGGYTGEGAKTVIASKAFAKISMRL
                     VPNQDWHEITELFTKHFESIAPKAVTVTVTPHHGGQGYVTPIDSIGYQAANKAYTETF
                     GVPAIPVRSGGSIPIVALFEKELKSKTILMGFGLDSDAIHSPNEHFGVFNYLKGIETI
                     PLFYKYFVEMSK"
     gene            1646..2065
                     /locus_tag="PK218_05995"
     CDS             1646..2065
                     /locus_tag="PK218_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98091.1"
                     /translation="MKKQHLTIIFLFLSCLLLSNCKINRTVNGLKHGKWITIDTVNNE
                     IYKRVEFYKKGDEIRTWKTHKNKKKYKTEKYKKGICLVTYYHPNGKIAIKGQTKQEVS
                     EKEIHWYYFGDWLFFDENGILTQTKIYENGTFINDFY"
     gene            2143..3243
                     /locus_tag="PK218_06000"
     CDS             2143..3243
                     /locus_tag="PK218_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HPW98092.1"
                     /translation="MLKHFFLLCSGVDSNIVNSCSNGEQNKYAGIGATVFFTAVMAFI
                     ASSYALFTVFNNPYIAMGFGLVWGFLIFNLDRFIVSTIKKRDSFLDEFIQATPRIALA
                     IVIAIVISKPLEIKIFEKEIAVVLLKEKNEMVMANKKQVTNYFQSDVDKNKAEIDSLK
                     SDITKKEKEVNDLYSVFITEAEGTKGTMKLGKGPVYKEKREKHDMALKQLDSLKAMNA
                     VKIKDKEAKAKTLQADLDKKVAETQPIIDGFDGLMARINALGKLPQLPSLFIMLLFLA
                     IETSPIIAKLLSPKSEFDFKQEDAEMGIKNVLSQNRYQSELQRKTDAEIYDKVYADIK
                     DDKELYNYKRKGALELLKLQADGFVEKQKKSM"
     gene            complement(3373..4074)
                     /locus_tag="PK218_06005"
     CDS             complement(3373..4074)
                     /locus_tag="PK218_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPW98093.1"
                     /translation="MIDNLKKILNEDQDPKAIEKITSKLNNLLMSNEEVGYIAVQKKP
                     AVTILPDSIVVTNKRIILCKPKNLGLSMEFIDYDWDDIAASFVKEGILGADFTFSTKS
                     DLTHTIDYLPKNQARKLYTYAKEQLDILKNGVQAPIVENGEKEETTYEAIPEIEETPT
                     EEVTNFAEIIPVSGYREEKQFPGDKTENTETGLGELSQDQLFEKLQNYKKLLDNGLIL
                     QGEYDTLKKEILSYM"
     gene            complement(4146..4517)
                     /locus_tag="PK218_06010"
     CDS             complement(4146..4517)
                     /locus_tag="PK218_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HPW98094.1"
                     /translation="MNIQQLYEFCLSKKGVTEHFPFDEDTLVFKVGGKMFCLTSLKEW
                     ENSTPSLNLKCDPEKAQELRAEYDAIKPGYHMSKIHWNTIDYNSDVNDRMMCELISHS
                     YDLVFKSLTKKLQNEILQIEN"
     gene            complement(4552..4959)
                     /locus_tag="PK218_06015"
     CDS             complement(4552..4959)
                     /locus_tag="PK218_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW98095.1"
                     /translation="MITVSTDKTKLNIPFIQNFLKDVYWAAGRTIDEVQTTIDASFCF
                     GIYLNDNQIGFARVITDYVVFAYVMDVFIDDNHRGKGYSSVLINAMMTEPELQQVKIW
                     RLATSDAHFLYEKFGFNSLAHPEKMMEKVISKQ"
     gene            complement(4979..5332)
                     /locus_tag="PK218_06020"
     CDS             complement(4979..5332)
                     /locus_tag="PK218_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW98096.1"
                     /translation="MITTVIPKLPFINKQKTLEFYINQMGFDLLSDYGDYLLMRKDKV
                     EIHFFSFESLDPKKSDFMIYLRIDAEIEKFYQELQDSEVEIHPNGTLETKPWNQKEFA
                     IIDPNGTLLTFGQAF"
     gene            complement(5347..6111)
                     /locus_tag="PK218_06025"
     CDS             complement(5347..6111)
                     /locus_tag="PK218_06025"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPW98097.1"
                     /translation="MLAKINNKYEIDLSKPNDISIPLSNTDENPIAWYIDKPEIKPVV
                     FGEWIGKVAEGSSTNFNNIFFNPHGHGTHTECLGHITREFYSINQCLKQFFFTAQLIS
                     VQPENLDDDLIISKEIISTALDVTKNIESLIIRTLPNPTSKKSKKYSNTNPPYLSEDA
                     AIYIRELGIQHLLIDLPSVDREEDEGKLLAHKAFWNVKDVNNLNEDARMNCTITELIY
                     VDDSVEDGNYILNLQIASFENDASPSKPVLFKILEL"
     gene            complement(6163..6540)
                     /locus_tag="PK218_06030"
     CDS             complement(6163..6540)
                     /locus_tag="PK218_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPW98098.1"
                     /translation="MTENELSKIVFDCALKVHQSLGLGLLESAYEECLFYELKKTGLV
                     VEKQKALPLVYEEVRLDIGYRIDIIVENKLILEIKSVEALNDVHFAQLLTYLKLTDCK
                     LGLLINFNVTLIKNGIKRIVNNL"
     gene            complement(6681..>7406)
                     /locus_tag="PK218_06035"
     CDS             complement(6681..>7406)
                     /locus_tag="PK218_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HPW98099.1"
                     /translation="EAKECLALASQYFDNISLDLIYGIPGMSNEKWLQNIETALSFNI
                     PHISSYALTVEPKTALHTFIQKGIIPQPDDEVAQEHFQILVDKLTENNFIHYELSNFG
                     KENYFSKNNSSYWLGKKYIGIGPSAHSYDGEKRGWNVSNNTLYIKSIQENKLPLETEI
                     LSKTDRYNEYIMTGLRTIWGISLDRIENEFGKSYLDYLNQQSAKYIEDHLLFVDENVL
                     RTTKNGKFLSDGIASDLFLLNLE"
     assembly_gap    7407..7506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7507..7757)
                     /locus_tag="PK218_06040"
     CDS             complement(<7507..7757)
                     /locus_tag="PK218_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPW98100.1"
                     /translation="MGFGLIKVVNKKMEFIQLNELQLSKYDNHYQKLKIIFERTIELI
                     ETHNPDEIAIEAPFFGKNVQSMLKLGRAQGVAMAAGLSR"
     gene            complement(7983..9275)
                     /gene="tyrS"
                     /locus_tag="PK218_06045"
     CDS             complement(7983..9275)
                     /gene="tyrS"
                     /locus_tag="PK218_06045"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPW98101.1"
                     /translation="MKNLVEELQWRGLVHDMMPGTEEQLLKEMTTTYIGFDPTSDSLH
                     IGSLVPIILLVHLGNFGHKPIALVGGATGMIGDPSGKSDERNLLDEVTLEKNVAGIKA
                     VLSRFLDFNSKATNAPILVNNYDWMKDFSFINFARDVGKRITVNYMMSKDSVKKRLET
                     EGEGMSFTEFTYQLIQGYDFHHLYKTHNCLLQMGGSDQWGNITTGTELVRRINGEGAK
                     AFAMTCPLITKADGSKFGKSEGGNVWLTADKTSVYKFYQFWLNTTDVDAEKYIKIFTF
                     LDKNTIDELIEEHQTAPHLRVLQKRLAEEVTIFVHGKEELEKAIFASNAFFSPTMDDL
                     KKLDETTFLEVFEGVPKAKVSKEDVENGLDMIAALSAKTNFLASNSDARRELKQNSIS
                     LNKERVVEDYIISSKDLINNQFLMLQKGKKNYYLIQVM"
     gene            9347..10342
                     /locus_tag="PK218_06050"
     CDS             9347..10342
                     /locus_tag="PK218_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPW98102.1"
                     /translation="MVLVTGGTGLVGAHLLLHLTESEDAIVAIYRNEKSIVKPKLLFQ
                     QFQKEAEFSKIIWIEADILDIPSLEIAFQNIEYVYHCAAQISFDPKDENLLRKVNIEG
                     TANIVNFCLDKNVKKLCHVSSIAALGDLKEHETILTEEIEWNPEVSHSDYAITKYGAE
                     MEVWRGQQEGLKVVIVNPGVIFGSTIWKTSSGTFFTKVKNNFPFYTYGTTGYVGVFDV
                     VKIMVLLMKSDSNGERFSLISENLSFKEIIHLIAGKMNAKKPTFEIKPWMTSIIWRVD
                     WFFSTVFKTRRNLSKNASKTIHSKEFISNAKIKNALNYNFQSIVEVVEIVLSKNQ"
     gene            complement(10336..10725)
                     /locus_tag="PK218_06055"
     CDS             complement(10336..10725)
                     /locus_tag="PK218_06055"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HPW98103.1"
                     /translation="MKKLILFFTILFIFGCQNSTVEKPSNLIEKDKMIAILYDVSLLE
                     AIKSQNINGGITPEEINEYIFKKYKIDSIQLVKSNKYYASDIVEYKKMYQKVKDQLEE
                     ESKKNEGKTGEINNTQDTKNSDTPTVY"
     gene            complement(10735..12075)
                     /locus_tag="PK218_06060"
     CDS             complement(10735..12075)
                     /locus_tag="PK218_06060"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPW98104.1"
                     /translation="MNTTLIKNAKIVNEGEIFEGDVLIENEYIVEIAQSISPKSANCK
                     IIDAEGNYLIPGAIDDQVHFREPGLTHKGDIESESRAAVAGGITSFIEQPNTLPNAIT
                     QELLEDKYQIAAQKAYANYSFMMGGTNDNLEELLKTNPRNVAGIKLFLGSSTGNMLVD
                     NPESIEKIFSSTKMLIAVHCEDETTIKNNLEHYKEQYGDAIPVACHPLIRSEEACYSS
                     SSKAIELAKKTGARLHVFHLSTAKEMELFTNKIPLEEKQITAEVCIHHLWFSDEDYKT
                     KGNFIKWNPAVKTANDRKVLWEALLDDRIDVIATDHAPHTLEEKSQDYLKAPSGGPLV
                     QHAVVAMFEAYHQGKISIEKIVEKMCHNPAKIFKIENRGFIKEGYFADLVIVNTGLPW
                     NVKKENILAKCGWSPFEGYNFKSRITHTFVNGTLVYQNFKVKEIKAGQRLLFNR"
     gene            complement(12080..12805)
                     /locus_tag="PK218_06065"
     CDS             complement(12080..12805)
                     /locus_tag="PK218_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPW98105.1"
                     /translation="MANGIVIIPTYNEIENIENIIRTVFLLETVFHVLVVDDNSPDKT
                     AEKVKELQLLFPEKLFLEVRKKKSGLGTAYVHGFKWAMKKEYDYIFEMDADFSHNPND
                     LEKLYLECKNNGADIAIGSRYVKGVNVINWPLSRVLLSYFASVYVRMITGMKIMDATA
                     GFICYKRNVLETLKLDRIKFIGYAFQIEMKYRAFVRNFKIHEVSVTFTDRTKGQSKMS
                     SSIIKEAVFGVISLRIRKLFNRL"
     gene            12912..13577
                     /locus_tag="PK218_06070"
     CDS             12912..13577
                     /locus_tag="PK218_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HPW98106.1"
                     /translation="MIEAVISPRILEPKDWATLLFLFSLLLIAIAKTAFETRFSEFLR
                     ILVSDKYIKIYKDSSHLMSGFTILLFIVQIISVSLFLQIILDHYGFVSKYDWISFLRI
                     TTFFSVFVLSKYLIEKIIATTFNFEEFVEQFNLLKVSYRTFLSLVLLPISIFLFYNKY
                     YADSLIYFIIIMILIINMLTYLVSLKNYQNLIIGNIFYFILYLCALEIAPYYFIYYLI
                     TKQ"
     gene            13595..14344
                     /locus_tag="PK218_06075"
     CDS             13595..14344
                     /locus_tag="PK218_06075"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPW98107.1"
                     /translation="MKVKTILVSQPEPKVENSPYFELQNKHKIKVDFRSFIHVEGVSA
                     KDIRAQKIDLNNFSAIILTSRNSVDHFFRVAEEMRYKIPEDLKYFCQSEAVAFYLQKY
                     VVYRKRKIYVGQKDFVDLSPLIKKYKDEKFLLPASDQLNFDVPQTLNNLKVSWTQATF
                     YKTVMSDLSDLADVYYDVLAFFSPTGIKSLFKNFPDFKQNNTRIAVFGSTTQKEALDC
                     GLRIDILAPTPETPSMTMALEKYIIEVNKVK"
     gene            complement(14414..15148)
                     /locus_tag="PK218_06080"
     CDS             complement(14414..15148)
                     /locus_tag="PK218_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98108.1"
                     /translation="MNYIYFLFLPMLFFGQTKPVKINIISVTSIDSIPEERKFTINYS
                     IENTTDKEISFFLQPEKLIPAHSNAMGTNVFYKLFQGKDELIIDGVFSTKKFLSFEGF
                     PDFSHITNEKEREEAAKKYFDEYMKKENDSIRKYTTVLTSNEIGYKIKNSKQLLNSIY
                     TLKPKETKTYSIIWYWDKKRYFKRDEFEYYLDEKGTFYIQFYLFLMKEQYRDKLIAID
                     YETLLKIPNFIKGVFQSEKVEINFKE"
     gene            complement(15188..16168)
                     /locus_tag="PK218_06085"
     CDS             complement(15188..16168)
                     /locus_tag="PK218_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HPW98109.1"
                     /translation="MIQSQDIFTLTSQKQFDKLALKVFRFQYQNNKVYREFCDFLKTN
                     PQEVKTIQQIPFLPIQFFKSHQIISSSETIQETFTSSGTTGMITSKHLVTDISLYEQS
                     FRNAFSEFFGNIEDYVVLALLPSYLERDGSSLIYMVNDLIERSNNEHSGFCLNNYDEL
                     ISKLVSLDNSGENVLLIGVTYALLDLIEKQNFQLKNTIIMETGGMKGKRKEIIREELH
                     ATLSKGFGVQNIHSEYGMTELLSQAYSFGNGIFECPNWMQILIRDTEDALSYVDSGKT
                     GGINVIDLANINSCSFIATQDLGKKHPNNSFEVLGRFDNSDIRGCNLLVL"
BASE COUNT         5360 a         2552 c         2406 g         5839 t
ORIGIN      
        1 tacacgcctt gcggcaccag atcctaagtc tggcatgtct accaatttca ccatacccgc
       61 aaaattgtga gtgcaaatat aatcaataat tttaaattac aaacttcaaa tcccaaattc
      121 caaataaata ataaatttgg aatttgggat ttcgcttttg ggatttctat ttgtatattt
      181 gaagttataa aacagttaac acaatggaca acatcaaaca atacgttcaa gaaaacaaag
      241 aacgatttat caacgaatta attgaattat taaaaattcc ttctgtaagt gcggatagtg
      301 cttatgcaca tgatgtactt gcaacagcag atgccgtaaa agaaagcttg aaaaatgcag
      361 gttgccaaca cgttgaaata tgcgaaactg aaggttatcc aatcgttttt ggaaaacata
      421 tcattgacga aaatttacct actgtcttgg tttacggaca ttatgatgtt caacctccag
      481 acccaataga actttggact tcacctccat ttgaacctgt aatcaaagca actgaaattc
      541 atcccgaggg tgctattttt gctcgtggag cttgtgatga taaaggtcaa atgtatatgc
      601 atgtaaaagc ttttgaatac atgattaaaa acaattgttt accttgtaac gttaaattta
      661 tgattgaagg tgaagaagaa gtcggctcta aaagcttagg cacatttgta gaaaacaatc
      721 aagagaaatt ggcaaatgat gtaattttaa tttcagatac tggaatgatt tctaaccaac
      781 agccaagtat tacaactggc ctacgtggtt taagctatgt agaagtggaa gtaactggtc
      841 caaatcgtga tttacattca ggattatacg gtggcgcagt agcaaaccca attaacattt
      901 tatcaaaaat gattgcttca cttcatgatg aaaacaatca tatcaccatt cctggttttt
      961 atgataaggt ggaagaactt tctgctgctg aaagagccga aatggcaaaa gcgccattta
     1021 gtatagaaaa ttataaaaaa gctttagaca ttaatgatgt ttttggtgaa acgggttatg
     1081 taaccaacga aagaaactct attcgtccaa cattagatgt aaacggaatt tggggtggtt
     1141 atactggcga aggtgcaaaa acagtaatcg ctagcaaagc atttgcaaaa atttctatgc
     1201 gattggttcc aaatcaagat tggcatgaaa ttacagaatt gttcacgaaa cattttgaaa
     1261 gcatcgcgcc aaaagcagta acagtaaccg ttacacctca tcatggcgga caaggttatg
     1321 taacaccaat tgacagtatt ggttatcaag cagcaaataa agcttacact gaaacttttg
     1381 gagttccagc aattcctgtt cgttctggtg gaagtattcc aattgtagcg ttatttgaaa
     1441 aagagttaaa atccaaaaca atcttgatgg gatttggttt ggatagcgat gccattcatt
     1501 ctccaaatga acattttgga gtttttaatt acttaaaagg aattgaaaca attccgttgt
     1561 tttataaata ttttgtggaa atgagcaagt aaaatactaa aaactataaa aattcgttct
     1621 aatatagaat agattttttt ttaaaatgaa aaaacaacac ttaactataa tttttctttt
     1681 cttaagctgt ttgcttttat ctaattgtaa aattaatcga actgtaaatg gattaaaaca
     1741 cgggaaatgg attacaattg acactgtaaa caatgaaatc tataagcgtg ttgaatttta
     1801 caaaaaagga gatgaaattc gtacatggaa aacccataaa aacaagaaaa agtacaagac
     1861 agaaaaatat aaaaaaggta tttgcttggt tacttactat catccaaatg gtaaaattgc
     1921 cattaaaggt caaacaaaac aagaggtttc agaaaaagaa attcattggt attactttgg
     1981 cgattggttg ttttttgatg aaaatggcat attaactcaa accaaaatat acgaaaacgg
     2041 aacttttata aatgattttt attaattcat tgatttgaaa aacaataaat tataattttt
     2101 ggcgttatat ttgtatatca tcaatcatta aatcaatcaa tcatgttaaa acatttcttt
     2161 cttctttgtt ctggtgttga tagcaacatt gtgaactctt gttcaaacgg tgaacaaaac
     2221 aaatatgccg gaattggcgc taccgttttc tttacagcag ttatggcatt tattgccagt
     2281 agttatgcgc tttttacagt ttttaacaat ccatatattg ccatgggttt tggattagtt
     2341 tggggttttc taatttttaa tttagaccga tttatcgttt ctactattaa aaaaagagac
     2401 agttttttag acgaatttat tcaagcaaca ccacgaatag ctttggcaat tgtaattgca
     2461 attgtgattt caaaaccatt ggaaattaaa atttttgaaa aagaaattgc tgtggttttg
     2521 ttgaaagaaa aaaacgaaat ggtaatggca aacaaaaagc aagtaactaa ttatttccaa
     2581 agtgatgttg acaaaaacaa agctgaaatt gatagtttga aaagcgatat tactaaaaaa
     2641 gaaaaagaag taaacgattt atattccgtt tttattaccg aagctgaagg cacaaaagga
     2701 actatgaaac taggaaaagg tccagtttat aaagagaaac gtgaaaaaca cgatatggcg
     2761 ttgaaacaac tcgattcgct caaagcgatg aacgccgtaa aaataaaaga caaagaagct
     2821 aaagccaaaa ctttacaagc tgatttagat aaaaaagttg ccgaaaccca acctataatt
     2881 gatggctttg atgggttaat ggctcgtata aatgcacttg gaaagttacc tcaacttccg
     2941 tcattgttta ttatgttgtt gtttttagca atagaaactt cgccaattat tgccaaatta
     3001 ttatctccaa aaagtgaatt tgattttaaa caagaagatg ccgaaatggg aattaaaaac
     3061 gtattgtctc aaaatcgtta ccaaagtgaa ttgcaacgta aaaccgatgc tgagatttat
     3121 gataaagtat atgctgacat taaagacgat aaagaattgt acaattacaa acgaaaaggc
     3181 gcattggaat tattgaaatt gcaagctgat ggatttgtag aaaagcaaaa gaagtcaatg
     3241 taaaatgtaa ccgcaaagtt cgcaaagtat tttcgcaagg ttcgcaagga ttttacaaat
     3301 acaaaaccca acataatgtt gggtttttct ttgcgttctt agcgtaattc tatgcgttct
     3361 tagcggttaa acctacatat aactcaaaat ttcttttttc aaagtatcat attctccttg
     3421 aagaatcaat ccgttatcta aaagtttttt gtagttctgt aatttttcga aaagttggtc
     3481 ttgtgataat tctcccaaac ctgtttctgt gttttcagtt ttgtcgccag gaaattgttt
     3541 ttcctctctg tatccagaaa ctggaataat ttcggcaaaa tttgtaactt cttctgttgg
     3601 agtttcttca atctcaggaa ttgcttcata agtagtttct tctttttcac cattttcaac
     3661 aattggggct tgtactccgt tttttagaat atccaattgt tcttttgcgt aagtatataa
     3721 ttttcgcgct tgatttttag gcaaatagtc aatggtatgc gtcaaatctg atttggttga
     3781 aaaagtaaaa tcggcaccaa gaattccttc ttttacaaaa cttgctgcaa tatcatccca
     3841 atcataatcg ataaactcca ttgataaacc caagtttttt ggcttacaaa gaattattct
     3901 tttattagta accacaatac tatcaggtaa aatagtaact gctggctttt tttgaactgc
     3961 aatatatcct acttcttcat tagacatcaa aagattattc aatttgctag tgattttttc
     4021 aatagctttt ggatcttgat cttcgtttag tattttcttt aaattatcaa tcattgaaaa
     4081 atggttaaaa gtttttgttt aaaagtttat aaggttttgg aataatttta atacaaatgt
     4141 aatgcctaat tttctatttg cagaatttca ttttgtaatt tttttgttaa acttttgaaa
     4201 accaaatcat acgaatgact tatcaattcg cacatcattc tgtcattcac atcgctatta
     4261 taatctatag tattccaatg aattttactc atatgatacc caggttttat tgcatcatat
     4321 tcggcacgaa gttcttgagc tttttctgga tcacatttta gatttaatga tggtgtgcta
     4381 ttttcccatt cttttaatga tgttaagcaa aacatctttc cgccaacttt aaacactaag
     4441 gtgtcttcat caaaaggaaa atgttcggtt actccttttt tagacaaaca gaattcgtaa
     4501 agttgttgaa tgttcatagt gatttttgat ttaatgctta ttaaatactg attactgctt
     4561 actaataact ttctccatca ttttttctgg atgtgctaat gaattaaatc cgaatttttc
     4621 atataaaaaa tgagcgtcag atgttgctaa tcgccaaatt ttaacttgtt gcaattctgg
     4681 ttcagtcatc attgcattta ttaaaaccga tgaatatcct ttgccacgat gattgtcatc
     4741 aataaaaaca tccataacat aagcaaaaac tacatagtct gtaattactc ttgcaaaacc
     4801 aatttggttg tcatttaaat aaataccaaa acaaaaagaa gcgtcaattg tggtttgcac
     4861 ttcgtcaatt gttcttcctg ctgcccaata aacatctttc aaaaaatttt gaatgaaagg
     4921 aatgttgagt ttggttttgt ctgtggaaac ggttatcatt tgattaacaa tttattattt
     4981 aaaaagcttg tccaaaagtt aataaagttc catttgggtc aataattgca aattcttttt
     5041 ggttccaagg ttttgtttct aatgtaccat ttggatgaat ttcaacttct gaatcttgca
     5101 attcttgata aaatttttca atttcagcat caattctcaa gtaaatcata aaatcagatt
     5161 tttttgggtc taaagattca aaagaaaaaa agtgaatttc tactttgtct tttctcatta
     5221 ataggtaatc tccatagtct gaaagtaaat caaaacccat ttgattgatg taaaactcta
     5281 atgttttttg tttatttata aatggaagtt taggaattac ggttgttatc atttgacaaa
     5341 caatttttaa agttctaaaa tcttaaataa tactggttta cttggacttg catcattttc
     5401 aaacgatgca atttgaagat ttaaaatata atttccatct tcaactgaat catcaacata
     5461 aattagttcc gttattgtgc aattcattcg cgcatcttcg tttaaattat tgacgtcttt
     5521 tacattccaa aatgctttgt gtgccaataa ttttccttca tcttcttctc tatcaacgct
     5581 tggcaaatca attaataaat gttggattcc caattctcga atataaatgg ctgcatcttc
     5641 tgaaagatat ggcggatttg tattagaata tttttttgat ttttttgatg tcggatttgg
     5701 taatgttcga atgataagcg attctatatt ttttgtcaca tcgagcgcag tcgagatgat
     5761 ttctttacta atgatcaaat catcatctaa attttctggt tgaaccgaaa ttaattgtgc
     5821 tgtaaagaaa aactgcttca aacattgatt gatgctgtaa aattctcgtg taatgtgtcc
     5881 gagacattca gtatgtgttc catgtccgtg cggattgaag aaaatattat tgaaatttgt
     5941 cgaacttcct tctgctactt tgccaatcca ttcgccaaaa acaacaggtt ttatttcagg
     6001 tttatcaatg taccaagcaa ttggattttc atctgtattg gataatggaa tagaaatatc
     6061 attaggtttt gataagtcga tttcgtattt attgttgatt tttgctaaca tattttttaa
     6121 ccgctaagag cgcaaagatt ttacgcaaag ttcgctaagt atttaaagat tattcactat
     6181 tcgcttaatt ccgtttttta ttaaagttac attgaaatta atcaataaac ctagcttgca
     6241 atcagtcaat tttaaatatg tcaaaagctg agcaaaatga acatcattta aagcttcgac
     6301 agattttatt tctaaaatta atttattttc aactataata tctattctgt aaccaatatc
     6361 caatctaact tcttcgtaaa ctaacggcaa tgctttttgt ttttcaacta caagtcctgt
     6421 tttctttaat tcataaaaca aacattcttc gtaagcactt tctaacagtc caagtccaag
     6481 actttgatgc acttttaaag cacaatcaaa tacaattttt gataattcgt tttctgtcat
     6541 aatactcatt ttggcgttta acaattactt attttaacca caaagagtgc aaagatttta
     6601 cgcaaagttc gctatgacat tttaacttct tttttttctt tgcgttcatt acgtaattct
     6661 ttgcggactt tgcggttaag ctattccaaa tttaataaaa ataagtctga agcaattcca
     6721 tcactcaaaa actttccatt ttttgtagtt cgcaatacat tttcatctac aaaaagcaaa
     6781 tgatcttcaa tatattttgc tgattgctgg ttgagataat ctaaatagga ttttccaaat
     6841 tcattttcaa ttctgtctaa tgaaataccc caaattgttc gtaaacctgt cattatatat
     6901 tcgttgtatc tatcggtttt tgataaaatt tcagtttcta atggcagttt gttttcttga
     6961 attgatttga tatataatgt attatttgaa acattccaac ctcgtttttc gccatcataa
     7021 ctatgcgctg aaggtccaat tccgatgtat tttttaccca accaataact cgaattgttt
     7081 tttgaaaaat agttttcttt cccgaaattg gataattcgt aatggataaa attattttcg
     7141 gttaatttat caactagaat ttgaaaatgt tcttgggcaa cttcatcgtc aggttgcgga
     7201 ataattcctt tttggataaa agtatgcaaa gccgtttttg gctcaacggt taaggcataa
     7261 ctcgagatgt gtggaatatt aaatgacaat gccgtttcga tattttgaag ccatttttca
     7321 ttactcattc ctggaattcc ataaatcaaa tccaatgaaa tattgtcaaa atattgtgat
     7381 gctaatgcta aacattcttt ggcttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnntctc ttgaaagccc agctgccatt gcaacgcctt gagctcttcc aagttttaac
     7561 atggattgca catttttacc aaaaaaaggt gcttcaatag ctatttcgtc agggttgtgt
     7621 gtttcgatta actcaatcgt tcgctcgaaa atgattttca gtttttgata atgattgtcg
     7681 tatttagaca actgcaattc gttcaactga atgaattcca tttttttatt gaccactttt
     7741 atcagcccga aacccataat tgttgttccg ggatcgattc ctagtatgat tctttcgttt
     7801 accaatttaa gatataaatt tatgtaaaaa taaagaactt atttgttaac aaataaaaat
     7861 gtcgagatat attataaagc ttcaaaaaaa agaatcaaca taaagcataa atagttgatt
     7921 tttagaactg ttctttcgag cctgcttttt tagtttataa aagactaata cgctttctat
     7981 ctttacatca cttgtatcaa atagtagttt ttctttcctt tttgcaacat caaaaattga
     8041 ttattgatta aatcttttga agaaataata tagtcctcta caactctttc tttatttaac
     8101 gatattgaat tttgtttcaa ttcgcgtcgg gcatcactat tggaagctaa aaaatttgtc
     8161 tttgccgaaa gtgcagcaat catatccaat ccgttttcta catcttcttt tgaaacttta
     8221 gcttttggaa caccctcaaa aacctctaaa aatgttgttt catctagttt tttcaaatca
     8281 tccatggttg ggctaaaaaa ggcattggaa gcaaaaattg ctttttctaa ttcttctttt
     8341 ccatgaacaa aaatggtaac ttcttctgcc aaacgttttt gcaatactct taaatgtgga
     8401 gccgtttgat gttcttcaat caattcatca attgtgtttt tgtctaaaaa tgtaaagatt
     8461 ttaatgtatt tctcagcatc aacatcagta gtattcaacc aaaattggta aaatttatac
     8521 accgaagttt tatcggcagt taaccaaaca ttgcctcctt ctgattttcc aaattttgaa
     8581 ccatcagctt ttgtaatcaa tggacaagtc attgcaaaag cttttgcacc ttcgccatta
     8641 attcttctaa ccaattcggt tcctgttgtg atatttcccc attgatcgct tcctcccatt
     8701 tgtagtaaac aattgtgtgt tttatacaaa tgatgaaaat catatccttg aattaattga
     8761 tatgtgaatt ctgtgaagct cattccttcg ccttcagttt ctaacctttt tttgacggaa
     8821 tcttttgaca tcatgtagtt gactgtaatt cttttaccta catcacgagc aaaattgata
     8881 aaagagaaat ctttcatcca gtcgtaatta tttactaaaa ttggtgcatt tgttgcttta
     8941 gaattaaaat ctaaaaaccg agaaagtaca gcttttattc ccgcaacatt tttttctaaa
     9001 gtcacttcgt ccaaaaggtt tctttcgtct gattttccag aaggatcgcc aatcattcct
     9061 gttgcaccac caaccaaagc aataggtttg tgtccaaagt tgcctaaatg aaccaataga
     9121 ataattggca ctaaacttcc aatgtgcaac gaatcacttg ttggatcaaa gcctatataa
     9181 gtggttgtca tttcttttaa aagttgttct tcggttccag gcatcatatc atgaaccaat
     9241 ccacgccatt gcaattcttc tactaaattt ttcattttta aaacaatttt cgcaaagata
     9301 gtttttagat ttggaatttg aaatttgaaa gttgtaaatt agcttcatgg ttttagttac
     9361 tggtggaaca ggattggttg gtgcacactt attacttcat ttgacagaaa gtgaagatgc
     9421 aattgtggcg atttatcgaa atgaaaaatc gatagttaaa cctaaattac tttttcaaca
     9481 attccaaaaa gaagctgaat tttctaaaat tatttggatt gaagctgaca ttttagacat
     9541 tccttcattg gaaattgctt ttcaaaatat agaatatgtt tatcattgtg cagcacaaat
     9601 atcattcgac ccaaaggatg aaaatttact tcgtaaagta aacattgaag gaacagctaa
     9661 cattgtaaat ttttgtttgg ataaaaatgt taaaaagctt tgtcacgtaa gttctattgc
     9721 cgctctaggt gatttaaaag aacacgaaac cattttgacc gaggaaatag aatggaatcc
     9781 cgaagtttct catagcgatt atgctattac aaaatatggc gctgaaatgg aagtttggcg
     9841 aggacaacaa gaaggcttaa aagttgtaat agttaatcct ggtgtgattt ttggttctac
     9901 gatttggaaa acaagtagcg gcactttttt tactaaagtt aaaaataatt ttccttttta
     9961 tacttatggt acaacgggtt atgttggtgt ttttgatgtt gttaaaatca tggtgctatt
    10021 aatgaaaagt gatagcaatg gcgaacgatt ctcgttaatt tctgaaaatt tatcatttaa
    10081 agaaatcatt catttaatag ctggaaaaat gaatgcaaaa aaaccaactt ttgaaataaa
    10141 accttggatg acatcaatta tttggagagt tgattggttt ttttctactg tttttaaaac
    10201 acgaagaaat ctttctaaaa acgcatccaa aactattcat tctaaagaat tcattagtaa
    10261 tgccaaaata aaaaatgctt tgaattataa ttttcaaagc attgtagaag ttgttgaaat
    10321 agtactttct aaaaatcaat aaaccgttgg tgtgtcagaa tttttagtgt cttgtgtatt
    10381 atttatttcc cctgttttac cttcattctt tttactttct tcctcaagtt ggtctttaac
    10441 tttttgatac atttttttgt attcaacaat atcactggca taatatttat tgctttttac
    10501 taactgaatg ctgtctattt tgtatttttt aaaaatgtat tcattaattt cttctggagt
    10561 aatacctcca ttaatatttt gactttttat agcttctagt aatgaaacat catataaaat
    10621 agcaatcatt ttatcttttt caatcaaatt actaggtttt tctacggtag aattttgaca
    10681 accgaaaata aatagtatcg taaaaaacag aattaacttt ttcataaaag ggtattatcg
    10741 attaaataat aaacgctgtc cggctttgat ctcttttact ttaaaatttt gataaaccaa
    10801 cgttccgtta acaaaagtat gtgttattct tgatttaaaa ttatagcctt caaaaggtga
    10861 ccaaccacat tttgctaaaa tgttttcttt tttcacattc catggtaagc cagtatttac
    10921 aataaccaaa tctgcaaaat aaccttcctt tataaatcca cggttttcta ttttaaaaat
    10981 cttagcagga ttgtgacaca ttttttctac aatcttttca atagaaattt tcccttgatg
    11041 ataggcttca aacatagcaa ctactgcatg ttgtaccaac ggcccacctg atggtgcttt
    11101 taaataatcc tgacttttct cttctaaagt atgtggagcg tgatctgtag caataacatc
    11161 tattctatca tcaagcaagg cttcccaaag tacttttcta tcatttgccg tttttacagc
    11221 aggattccat ttgataaaat tacctttagt tttataatct tcatcactaa accaaagatg
    11281 gtgaatacaa acttcggctg tgatttgttt ttcttctaaa ggaattttat tggtaaacaa
    11341 ctccatttct tttgccgttg aaagatgaaa aacatgcagt ctagctcccg ttttttttgc
    11401 caattctata gcttttgaag aagaactata acaagcctct tcactgcgaa tcaacggatg
    11461 acatgctaca ggaatagcat cgccatattg ttctttgtag tgctccaaat tgttcttaat
    11521 agtagtttca tcttcacaat gaaccgcaat cagcattttg gtagaagaaa aaatcttttc
    11581 tatactttct ggattatcaa ctaacatatt tccggtagat gatccaagga ataattttat
    11641 tcccgcaaca tttcttggat ttgttttcaa taattcttcc aggttatcat tcgttcctcc
    11701 catcataaac gaataattgg catacgcttt ttgtgcagct atttgatatt tatcttctag
    11761 tagttcttgt gtaattgcat tgggaagagt attgggttgt tctataaaag aagtaattcc
    11821 tccagcaacc gcggctctgc tttcggattc gatatcacct ttatgagtca atccaggttc
    11881 gcgaaaatgc acttgatcat caattgctcc tggtattaaa taatttcctt cggcatcaat
    11941 gattttgcaa tttgccgatt ttggagaaat ggattgggca atttcaacaa tatattcatt
    12001 ttcgatcaat acatcgcctt caaaaatttc tccttcgttt acaattttag cattctttat
    12061 taacgtagta ttcatttttt tataatcgat taaaaagctt tcttattctc aaacttatca
    12121 caccaaaaac agcttctttg atgatagaac tactcatttt ggattgccct tttgttctgt
    12181 ctgtaaaagt taccgaaact tcgtgtatct taaaatttct aacaaaagcc cggtatttca
    12241 tttctatttg aaatgcatat cctatgaatt taattctatc gagttttaga gtttctagca
    12301 catttctttt gtaacaaata aaacctgctg tagcatccat aattttcata ccagtaatca
    12361 ttctaacata aaccgaagca aagtaagaaa gcaacacacg actcaaaggc caattgatta
    12421 cgttaacacc ttttacataa cgagaaccaa tagcaatgtc ggcaccatta tttttacatt
    12481 ctaaatataa tttctctaaa tcatttgggt tatgagagaa atcggcatcc atttcaaaaa
    12541 tataatcata ttcttttttc atagcccact tgaaaccatg tacataggcc gtgccaagtc
    12601 ctgatttctt ttttctaact tctaaaaata atttctcagg aaatagaagt tgtaactctt
    12661 ttactttttc tgctgtttta tcaggagaat tgtcatctac aacaagcaca tgaaaaacgg
    12721 tttcaagcaa aaaaacagta cgaataatgt tttcaatatt ttcaatttca ttatacgttg
    12781 gaataattac aatgccgtta gccataaatt tgaaaaagtt ttttaaaagg caaaaatact
    12841 ctttttatca aagttacttc ttaataatta tataataaaa attgaaaata aaaaattagt
    12901 agttttgcag aatgatagaa gcagtaatta gtcctagaat tttggaaccc aaagattggg
    12961 caaccctact ttttctattt tctttgttgt taattgcaat tgctaaaaca gcatttgaaa
    13021 cccgatttag tgagttttta agaatattag tttctgataa atatataaaa atctataaag
    13081 acagttcaca tctgatgagt ggatttacca ttcttttgtt tattgtacaa attatatcag
    13141 tttccctttt tcttcaaatt attcttgatc attatggatt tgtttcaaaa tatgattgga
    13201 taagtttttt gagaattact acttttttta gtgtttttgt tttgtctaaa tacttaatag
    13261 aaaaaattat tgcgactaca tttaattttg aagaattcgt tgaacaattt aatttattga
    13321 aagtaagtta cagaaccttc ttaagtttgg tactattacc tatttcaata tttctgtttt
    13381 ataacaaata ttatgcagat tcgttaatat atttcattat cattatgatt ttgataataa
    13441 acatgcttac ttatcttgtt tctttgaaga attatcaaaa tttaattatt ggtaatatct
    13501 tttattttat tttatatctt tgcgcacttg aaatagcgcc ctattatttc atatattatt
    13561 tgattacaaa acaataaaga gtactatgtc aaatctgaaa gtgaaaacaa ttttggtttc
    13621 ccaaccagag ccgaaggtag agaattctcc ttattttgaa ctgcaaaata agcacaaaat
    13681 aaaagtagat tttcggtctt ttattcatgt tgaaggcgta agtgcaaaag atattcgagc
    13741 tcaaaaaatt gatcttaata actttagtgc tatcatttta acaagccgaa actctgtaga
    13801 tcattttttt agagtagccg aagaaatgcg ttataaaatt ccagaagatt taaaatattt
    13861 ttgtcaatcg gaagccgttg cattctatct acaaaaatat gtagtgtata gaaagcgtaa
    13921 aatttatgtg ggtcagaaag attttgttga tttatcacca ttaattaaaa aatataaaga
    13981 cgaaaagttt ttattacctg cttctgatca attgaatttt gatgtaccac aaaccttgaa
    14041 taatttaaaa gtttcttgga cacaggccac tttttacaag acagtgatga gtgatttgtc
    14101 tgatttagct gatgtgtatt atgatgtttt agcttttttt agtccaactg gaattaaatc
    14161 tttattcaaa aattttcctg atttcaaaca aaacaatact agaattgcag tttttggaag
    14221 tactactcaa aaagaagcgc ttgattgtgg tttgcgaata gatattcttg cgccaacacc
    14281 agaaacacca agtatgacaa tggctctaga aaaatatatt atagaagtta ataaagtaaa
    14341 ataaaaaatc ttcaaaaaaa gcaacctcaa aataacatct atcatttggc aggagaacgc
    14401 tttttaattt tttttattct ttaaagttga tttctacttt ttcagattga aacacacctt
    14461 taataaaatt tggaattttt agcagtgttt cataatcaat agcaattaat ttgtctctat
    14521 attgctcttt catcaaaaat aagtagaatt gaatatagaa agtccctttt tcgtctaagt
    14581 aatattcaaa ttcatcacgt ttgaaatagc gttttttatc ccaataccaa attattgaat
    14641 aggttttggt ttctttgggt tttagagtat atattgaatt caacagttgt ttactgtttt
    14701 ttatcttata accaatttca tttgatgtta aaacagtagt atattttctg atggaatcat
    14761 tttctttttt catatactca tcaaaatact ttttcgcagc ttcttctcgt tctttctcat
    14821 ttgtgatatg agaaaaatcg ggaaatcctt caaaggataa aaatttttta gtgctaaaaa
    14881 caccatcaat tatcaactca tcttttcctt gaaacaattt gtaaaaaaca tttgttccca
    14941 tagcatttga atgtgctgga attaattttt cgggttgaag aaaaaatgat atttctttgt
    15001 ctgttgtatt ttctatcgaa tagttgattg taaattttcg ttcctctggt attgaatcta
    15061 ttgaagtaac agaaataatg ttgattttaa cgggttttgt ttgcccgaaa aaaagcatgg
    15121 gtaaaaacag aaaataaata tagttcaatt ttgtcatata tataataacg aaaaaagata
    15181 gaaaatatta taaaaccaac aaattacaac cgcgaatatc cgaattatca aatcgtccta
    15241 aaacttcaaa tgaattgttt gggtgctttt ttcctaaatc ttgtgtagca ataaacgaac
    15301 acgaattaat attggccaaa tcaattacgt ttattccacc cgtttttcca gaatctacat
    15361 aactcaacgc atcctcggta tcgcgaatta gtatttgcat ccaatttgga cattcaaaaa
    15421 ttccgtttcc aaaggaatag gcttgtgata acaattcggt cattccatat tctgaatgga
    15481 tattttgaac tccaaaacct ttggataacg tagcgtgtaa ttcttctcgg atgatttctt
    15541 ttcgtttgcc tttcattcca ccagtttcca ttatgatggt gttttttaat tggaaatttt
    15601 gtttttcgat taaatccaat aaagcatagg ttacaccgat taacaaaacg ttttctccag
    15661 agttatctaa tgaaactaat tttgaaatta attcgtcata attattcaaa caaaaacctg
    15721 aatgttcatt attagatctt tcaattaaat cgtttaccat ataaatcaaa gaagagccat
    15781 cacgttctaa ataagacgga agcaatgcta aaacaacata atcttcaatg tttccaaaaa
    15841 attctgagaa agcattgcga aaactttgtt catataatga aatatcagta actagatgtt
    15901 tgcttgtaat cattcctgtt gttccgctgc ttgtaaatgt ttcttggata gtttcggacg
    15961 acgaaataat ttgatggctt ttgaaaaact gaatgggtaa aaatggaatt tgttgaattg
    16021 tttttacttc ttgtgggttt gttttaagaa agtcacaaaa ttcacgataa actttattat
    16081 tttggtattg aaaccgaaaa actttcaaag ccaatttgtc aaattgtttt tgagaggtta
    16141 gagtaaatat atcttgcgac tgtatcaact ttttttttga caaaagtaat gaaaataaaa
    16201 aagctctaac tttcgttaga gctttaaaat tattatagaa ttataattat ctaataa
//
LOCUS       DAOOBH010000043        16188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_4698_length_16188_cov_6.037935, whole genome shotgun sequence.
ACCESSION   DAOOBH010000043 DAOOBH010000000
VERSION     DAOOBH010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 16188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16188
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4698_length_16188_cov_6.037935"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..1657)
                     /locus_tag="PK218_06090"
     CDS             complement(272..1657)
                     /locus_tag="PK218_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPW98110.1"
                     /translation="MKKISSLFLVSLFSGAITLGAYKVFFEKENKTGITTLATPNYSK
                     SVGLTSEGMDFTLAAENTVHTVVHVKNVSVRTVYNPIMEYFYGSRGGQQQEQIGTGSG
                     VIISEDGYIVTNNHVIKDATDLEVTLNNNKSYKAKLIGTDSKMDIALLKIDANGKLPY
                     ATFADSDQVKVGEWVLAVGNPYNLTSTVTAGIVSAKARNLDTKGIQSFIQTDAAVNPG
                     NSGGALVNSRGELIGINTMISSPTGSYTGYSFAVPSNITRKIIEDIMEYGNVQRGILG
                     VEGGELNSKVSKELGVNDTEGFYINKVTKNSGAEKAGLKTGDIIKKLDNQNINSFAEL
                     SGYISTKRPNDLVKVTFLRDGETKTTNVTLIKNDVFTTEFKGLELENIDATDKKKFRI
                     DYGVRIKEINNENLTPYYDQLKGNIILSVDNVKATDVESISTFLNKKDENRSVNIRMI
                     DKSGQVIQIII"
     gene            1791..2573
                     /gene="dapF"
                     /locus_tag="PK218_06095"
     CDS             1791..2573
                     /gene="dapF"
                     /locus_tag="PK218_06095"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPW98111.1"
                     /translation="MKLQFFKYQGTGNDFIMIDNRQLFFPKENTQLIEKLCDRRFGIG
                     ADGLILLENDDKTDFKMIYFNSDGNESSMCGNGGRCIVAFAKQLGVISNNTQFIATDG
                     LHHATINDKQTVSLQMKDVDEVKIESDYVFMNTGSPHHVTLIDSLENYDVKTNGAKIR
                     YSDLYGKVGSNINFVKQLNDYHFAVRTYERGVEDETLSCGTGVTAVAIAMNALGKTNS
                     REIDLDVQGGKLKVSFEKENQTYKKVHLIGPATFVFEGEIKL"
     gene            2623..3156
                     /locus_tag="PK218_06100"
     CDS             2623..3156
                     /locus_tag="PK218_06100"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPW98112.1"
                     /translation="MITLKGNNIYLRALEPEDLEFIYKIENDENIWEVSNTQTPYSKF
                     LIRQYLENAHQDIYEAKQLRLAICKNNSNEAIGLIDLFDFDPKNKRAGIGVIIQNETD
                     RNNGFGKEALSLLIDYSFLQLQLHQLFANIDTQNEPSISLFTTFGFEKIGVKKDWNFN
                     KKSFQDEALFQLIHHLK"
     gene            3164..4198
                     /gene="mltG"
                     /locus_tag="PK218_06105"
     CDS             3164..4198
                     /gene="mltG"
                     /locus_tag="PK218_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPW98113.1"
                     /translation="MNSKLKKILFTIVTLLIILVGFVYIKYFTNNTKIDQKEIFVKIP
                     TGATFEEVKKIMEPFVDNMNSFEFIAKRRSYNENVKAGRFLLKKGMSAFEQVATLRKN
                     VPVNLAFNNQERLENLCERLSAQIEPDTTQLLATFKDSIFLKQNGFTKETVFSMFLPN
                     TYQVYWNISAEKFRTKMLDEYKKFWNPERINKAEILELTPIQVTILASIVQKETAKKS
                     ERKTVAGVYLNRLKEGMPLQADPTVIYALKYRDNDFNQIIKRVLYNDLFISSPYNTYL
                     VTGLPPGPITMPDVDAIDAVLNAEKHNYLYFCASTEKFGYHVFAATYEQHQVNARKYS
                     AWLNKLGTKR"
     gene            4202..5119
                     /locus_tag="PK218_06110"
     CDS             4202..5119
                     /locus_tag="PK218_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HPW98114.1"
                     /translation="MSNIKSVLFVLLFIGFHNAWSQNGIDNYLKPSDTLDKKRLNAVI
                     VSETVVASATLIGLNQLWYADYPKSNFHFINDNEEWLQIDKAGHLFSSYHLGKIGADA
                     LKWSGVSKKNQLIYGSSLGLAFLTTVEVFDGFSANWGASFGDVVANVSGTALYISQEL
                     LWKEQRVIPKFSFHTTRYASLRPDVLGSSLNEQILKDYNGQTYWLSCNIHSFFKESKI
                     PKWLNVAFGYGAEGMITGDEDTGNAIFISKDERYRQFYLSFDVDLTKIETKSHVLKTL
                     FSVFNSLKIPAPTLEINGSHGVKVHLFYF"
     gene            5206..5841
                     /locus_tag="PK218_06115"
     CDS             5206..5841
                     /locus_tag="PK218_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HPW98115.1"
                     /translation="MIQKSIFYTSILLIVTFISFGFKPYDNQINQWFDFDSSDEALYI
                     FPSQQNTDYTYLNAPFTGKFFIGYKEAIGFKESQNKYQKVNTLGYMGKYQFGMETLKS
                     IGVSDSARFINNPKLQEKAFVTLLSRNKFILQNYIAKYEGKVISNIKITESGILAAAH
                     LGGAGSVIKFFKSKGERRVKDEYGTSIRSYLKEFAGYETKNIPAIKNARIK"
     gene            complement(5843..6556)
                     /locus_tag="PK218_06120"
     CDS             complement(5843..6556)
                     /locus_tag="PK218_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPW98116.1"
                     /translation="MNPKGKLYLIPTTMGEMNPDEVLPSLVYRTVELLTTYIVENEKT
                     ARKFIKSVQPSKIQADLKLSLLNKRTEDSELQSMIKPCLNGENIGLMSEAGCPGVADP
                     GAAIVKIAHEHGIQVVPLVGPSSILLAMMASGMNGQSFAFNGYLPIDKSEKKNAIKNF
                     ETLSHSKNQSQLFIETPYRNNKLMEDLLQFLQPNTSLCIACDITLPSEYIKTYRVADW
                     KKIKVDLHNRPTIFIIHKM"
     gene            complement(6553..7020)
                     /locus_tag="PK218_06125"
     CDS             complement(6553..7020)
                     /locus_tag="PK218_06125"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HPW98117.1"
                     /translation="MAVKILMVCLGNICRSPLAEGILASKLPKDKFMVDSAGTGNYHI
                     GKQPDSRSIAIAQKNGLDITNQRGRQFSSRDFDNFDYILAMDNSNYNDIIELAKTEEQ
                     KEKVDLILNHLFPGDNVDVPDPYYGLQNGFDMVYEMLDETCAILAKKLIEKHS"
     gene            complement(7062..8489)
                     /gene="dnaA"
                     /locus_tag="PK218_06130"
     CDS             complement(7062..8489)
                     /gene="dnaA"
                     /locus_tag="PK218_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPW98118.1"
                     /translation="MSKTAQSVWENCLSFIKDNIQEQAYKTWFEPIKSVELTDNALYI
                     QVPSKFFYEWLEEHYVKLLKVALTKELGKNAKLLYKIKMENTYGNKQPFTEQLPSAHR
                     SPIKSQDVDAPYKNISTELKNPFVIPGIRNLKIESQLNPNYSFDNFLEGDSNRLARSA
                     GMAVANKPGGTSFNPLLIFGGVGLGKTHLAHAIGVEIKDKYPEKTVLYISAEVFTQQY
                     IDSVKKNNRNDFIHFYQLIDVLIIDDVQFLSGKSGTQDVFFHIFNYLHQNGKQVILTS
                     DKAPVDMQDIEQRLLSRFKWGLSAELHQPDYETRISILKNILYRDGVEMPEEIIEYVA
                     RNIKTNVRELEGAIISLIAQSSFNKKEVTIDLAKSIVEKFVKNIKREISIDYIQKVVS
                     DYFQLDLDTLQSKTRKRHVVQARQLAMFFAKKFTKASLANIGSQIGDRDHATVLHACK
                     TVDNLVSTDKQFKKFVDDIHKKLSL"
     gene            8675..9076
                     /locus_tag="PK218_06135"
     CDS             8675..9076
                     /locus_tag="PK218_06135"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPW98119.1"
                     /translation="MKEHQVQLRVRYSETDQMGVVYHGSYVPYFEIGRVEWLRNKGVS
                     YKELEESGVALPIVSMTLNYKKPARYDDLLTVITKFKSQSSVKIEFDCEIRNENNELL
                     TTAHFILVFVDIKLGKAIAPPKAILELMKEG"
     gene            complement(9078..9680)
                     /locus_tag="PK218_06140"
     CDS             complement(9078..9680)
                     /locus_tag="PK218_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPW98120.1"
                     /translation="MKDSYLTIACPSSEILFKEKNSKFFGYAFPVTSEEDVKNCLDDL
                     KKEHNSARHFCYAYQIGTEEKQYRVNDDGEPNNSAGMPIYGQIQSFDVTNILIVIIRY
                     FGGTKLGVGGLISAYKTSAQMTLETAEIIKKTIDIEYKISFDYKNINKVMRVIKEKNL
                     DIVSQTMNESCEIIISTRKKNAEMIFDIFNNLFEIEVTQL"
     gene            complement(9746..10354)
                     /locus_tag="PK218_06145"
     CDS             complement(9746..10354)
                     /locus_tag="PK218_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPW98121.1"
                     /translation="MIEAIIFDFGDVFINLEKQKSIDEFKKLGLNGPNEDLIAHNDLF
                     EKGKITETEFINCFKKYIPEATSEEIISAWNSIIGEFPLHRLEFLQLLSTKYRLFLLT
                     NTDAIHINCFEENVCMSFYSDFYRCFEKVYYSYEMGMRKPQPEIFTTIVSKHNLNPSR
                     TLFVDDKKENTDAAAALGLYVWNLQVGKEDVVDLFEKKEFPL"
     gene            complement(10347..11387)
                     /gene="ribD"
                     /locus_tag="PK218_06150"
     CDS             complement(10347..11387)
                     /gene="ribD"
                     /locus_tag="PK218_06150"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPW98122.1"
                     /translation="MKIHEIYIKRCIQLAKNGLGTTYPNPLVGSVIVYNGKIIGEGWH
                     KKAGEAHAEVNAINSVKDKSLLSKATIYVSLEPCSHFGKTPPCADLIMKHKIPNVVIG
                     TIDPNPKVAGNGIKKLIENDIKITVGVLEKECYDLNKRFFTFQNKKRPYIILKWAESA
                     DGYIAPLTKEKKEPFWLTNEFSRQLVHKWRSEEQAILAGTNTVLEDNPKLNVRNWHGK
                     NPVRIVLDKSGKITSDYFVLDNQMKTIILTEQENLQSTENCIYENTIFDTQLAEKIGV
                     ILWKHNLQSVIIEGGTITLETFINASLWDEARVFKSNLEMKNGVKAPVLNHYSTLSKI
                     KNDNLTLFYNHD"
     gene            11416..11907
                     /locus_tag="PK218_06155"
     CDS             11416..11907
                     /locus_tag="PK218_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW98123.1"
                     /translation="MSKIVIREIQSKDNQQIAAVIREVFINDNYPKTGTAFADKQLDF
                     MFETYDKEKAIYFVIENDGKIIGGAGISQLDNADSNICELQKMYFLNEARGKGLGFKI
                     IQMCLEKAKEFGFEKCYLETLPEMKTAQHLYQKSGFEYIDAPMGNTFHNSCPVWMIKD
                     LSK"
     gene            11909..12772
                     /gene="prmC"
                     /locus_tag="PK218_06160"
     CDS             11909..12772
                     /gene="prmC"
                     /locus_tag="PK218_06160"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPW98124.1"
                     /translation="MLLKKYKSNFIEELSSIYDEMEAESFFYLILEHLHQFKRIDLAL
                     NPDFEISESDKVRWENILSELKTQKPIQYILGETEFYGLKFIVDENVLIPRPETEELV
                     DLIVSSFKSQTSKLRILDIGTGSGCIPISLKKNLPNAEVFAIDVSENALEIAKKNAKL
                     NGVEVNFILKNILETNDLEQQFDIIVSNPPYVRMLEKEEIKTNVLEYEPHLALFVEDN
                     DALIFYRKIAQLAKKSLTENGKLYFEINQYLGKETVILLEKMDFKNIELLQDIYGNDR
                     MISCSKTNNYS"
     gene            complement(12763..14007)
                     /locus_tag="PK218_06165"
     CDS             complement(12763..14007)
                     /locus_tag="PK218_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW98125.1"
                     /translation="MFKLFLENIKIALGSIRTQLLRTILTILIIALGIWALVGILTVV
                     SALENTLSANFASMGSNTFNINRYEFTARRNGGDEVQKVNPIISYPEAKAFKEHFDFP
                     STNTSISFYATSTAEVKFENEKTDPEISVVGVDEFYIPNSGLEVTQGRNFNKFDIDNN
                     TYSCVVGSDFATKGLLKDINPIGKILSVRGAKFKVIGVLKEKGSTFGNSQDLRMMIPI
                     QVARSMFSAPNINYTISSMVQQKEFLDAAINEALVTMRQVRKLNPVEDNNFGISRSDD
                     LINRIAEMTGVLNISAWVIGIITIFGSSIALMNIMLVSVTERTREIGVRKALGAKRNT
                     IAFQFFVETLIIGQLGGLVGILLGITTGYLIAVAIDFSFVIPWLAIMAAVLTTFIVAV
                     VSGSYPALKASRLDPIEALRYE"
     gene            14178..15824
                     /gene="hisS"
                     /locus_tag="PK218_06170"
     CDS             14178..15824
                     /gene="hisS"
                     /locus_tag="PK218_06170"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPW98126.1"
                     /translation="MAQKPSIPKGTRDFSPVEVSKRNYIIAVMRKHFENYGYQPIETP
                     SFENSDTLMGKYGEEGDRLIFKILNSGDYFEDVRKDVFYSSNINAVFFDIFDNLVQFD
                     VNKSDKYNLFLIKFLDVFSKNRITSSNEKNERSLIEKVFLENKDKILEFDYTDKPNLA
                     NFRRQIFTVFFKEYIEEFDVLNSQKGTKKISEKALRYDLTVPFARYVVQHQNEIEFPF
                     KRYQIQPVWRADRPQKGRFREFYQCDADVVGSTSLWQEVELVQLYDVVFNDLGLNGVT
                     IKINNRKILSGIAEVIGASDKLIDFTVALDKLDKIGEEGVKKEMLEKGISETALEKVQ
                     PLFNFTGTIDEKLAKLSALLSNSEEGKKGVEELRFICENVSKLGLQQANLDLDVTLAR
                     GLNYYTGAIFEVAAPKEVAMGSIGGGGRYDDLTGIFGLKNMSGVGISFGLDRIYLVLE
                     ELNLFPETVTNTTQVLFLNYGEKEAFEAMKTITTLRKENIKAELYPDASKIDKQFKHA
                     ERRGIPFVVKEIEGNNVTLKDLKTGEQLVLDVTHLIKKLS"
BASE COUNT         5447 a         2456 c         2704 g         5581 t
ORIGIN      
        1 aaaagcacca attcaattga tttgtcatac cataaatcgc taacgatttt aattaattct
       61 acgccagctt ttcttctgtc cgcttgaaaa gaaagttctt tttcgtataa gtttgttgta
      121 ctcataaaaa ggtttaagtt gttgtgttga aataaatttg gcgcaaaaat agtaaattca
      181 atcctttaac gaaaacgatt tcgttattat ttttaacaac aaaaaaacca gttcaaaatg
      241 gaactggttt taatgatttt taaccctttt tttatataat aatctgaata acttgccctg
      301 atttgtcaat cattctaatg ttaacgcttc tgttttcgtc ttttttattt aagaatgttg
      361 atattgattc tacatcggtt gcttttacat tatcaaccga aagaataata tttcctttaa
      421 gttggtcgta atatggtgtt aaattctcgt tattgatttc tttaattctt acgccataat
      481 caattctgaa tttctttttg tctgtcgcgt caatattttc aagttccaag cctttaaatt
      541 ctgttgtgaa gacatcgttt ttgattaaag ttacattggt agttttggtt tctccatctc
      601 taagaaaagt cacttttact aaatcattag gtcttttagt acttatgtat cccgaaagtt
      661 cggcaaaaga attaatgttt tgattgtcta actttttaat aatatcaccc gtttttaaac
      721 ctgctttttc agcaccagag tttttggtaa ctttattaat gtaaaatcct tcggtatcat
      781 taacacctaa ttctttagaa actttgctgt tcaactcgcc accttcaaca ccaagaatac
      841 cgcgttgaac attcccatat tccataatat cttcaattat ttttcgggtg atattagatg
      901 gaactgcaaa tgaatagcct gtgtaacttc ctgttggaga agaaatcatg gtgttaattc
      961 caattagttc gccacgagag tttactaatg caccaccaga atttccagga tttacggcag
     1021 catcagtttg tataaatgac tgaatacctt tggtatctaa atttcttgct ttagcagaaa
     1081 caattcctgc agtaactgtt gaagttaaat tataaggatt tccaacggct aaaacccatt
     1141 cgccaacttt cacttgatcc gaatctgcaa aagtagcata tggtaatttt ccatttgcat
     1201 caatctttaa taaggcaata tccattttag aatcggttcc aatcagtttt gctttgtatg
     1261 atttgttgtt gtttagcgta acttctaaat cagtagcgtc tttaatcacg tggttgttcg
     1321 tcacaatata accatcttca gaaatgatga ctccagaacc tgttccgatt tgttcttgtt
     1381 gctgtccacc tcgtgaacca taaaaatatt ccattatagg gttataaacc gttcgaacag
     1441 atacgttttt aacgtgaact acagtgtgta ctgtattttc agcagctaaa gtaaaatcca
     1501 ttccttctga agttaatcca accgattttg agtaattggg tgttgctaaa gttgtgattc
     1561 ctgttttatt ttctttttca aaaaatactt tatacgcacc aagagttatt gcgccactaa
     1621 atagagaaac taaaaataaa cttgatattt ttttcatatt tgttgagatt tataaaatta
     1681 atacagtaaa attaaaaatt tagaatattg gaaatatacc ttttaacggt cgtttaacat
     1741 tttttaagtt aatcttaata atcgttatct ttgtgtactt attttaaaaa atgaaactac
     1801 aatttttcaa atatcaaggc actggaaacg attttatcat gattgataat cgtcaattat
     1861 tttttccaaa agaaaatact caactcatcg aaaaactttg cgatagaaga tttggaatcg
     1921 gtgcagatgg attaatcctt ttagaaaacg atgataaaac ggattttaaa atgatttatt
     1981 ttaattctga tgggaatgaa agctcgatgt gcggtaacgg cggaagatgt atagtggctt
     2041 ttgccaagca acttggtgta atttccaata atacccaatt tattgcaaca gatggtttac
     2101 atcacgctac tattaatgac aagcaaacag tgtcattgca aatgaaagat gttgatgaag
     2161 tgaaaattga aagtgactat gtttttatga acactggttc gccacatcat gtcactttga
     2221 ttgatagttt ggaaaattat gatgtaaaga cgaatggtgc aaaaatcagg tattccgatt
     2281 tgtatggaaa agttgggagt aacatcaact ttgttaagca attgaatgac tatcactttg
     2341 ccgttagaac atatgagcgt ggcgtagaag atgaaacact ttcttgtgga actggcgtta
     2401 cggctgttgc tattgcaatg aatgctttag gtaaaacaaa ttctagagaa attgatcttg
     2461 atgttcaagg tggaaaacta aaagtttctt tcgaaaaaga aaatcaaacc tataaaaaag
     2521 tgcatttaat tggaccagca acttttgttt ttgaaggaga aattaaattg taattatata
     2581 tcattaactt cgtattttgt acttctaatt ttgtacctat aaatgataac cttaaaagga
     2641 aacaacatct acctgcgtgc actcgaaccc gaagatttag agtttatcta taaaatagaa
     2701 aacgatgaaa atatttggga agtcagcaat acacaaacgc cttatagcaa atttctaatt
     2761 cgtcagtatc tggaaaatgc ccatcaagat atttatgaag ccaaacaatt gcgattggca
     2821 atctgtaaaa ataattcgaa tgaggcaata ggtttaatcg acctgtttga ttttgatccc
     2881 aaaaataaac gtgcaggaat tggtgttatc atccaaaatg aaacggatag aaataatggt
     2941 tttggaaaag aagcattgtc acttttaatt gattattcgt ttttgcaact gcaattacac
     3001 caattatttg caaatattga tactcaaaac gaaccaagta tttctctttt tactacattt
     3061 ggttttgaaa aaataggagt aaaaaaagat tggaatttta acaaaaaatc cttccaagac
     3121 gaagcacttt ttcaactgat acatcattta aaataacttg attttgaatt caaaacttaa
     3181 aaaaattcta tttacgatag tgacgcttct gattatttta gttggatttg tttatattaa
     3241 atactttaca aacaacacaa aaatagacca aaaggagatt ttcgtaaaaa taccaactgg
     3301 tgcaactttt gaagaagtaa aaaaaataat ggaacctttt gtagataata tgaacagttt
     3361 tgagtttata gcaaaacgtc gttcgtataa tgaaaatgta aaagcgggac gatttttatt
     3421 gaaaaaagga atgagtgctt ttgagcaagt agctacttta agaaaaaatg ttcccgtaaa
     3481 tttagctttt aacaatcaag aacgattgga aaatctttgt gaacgcttga gcgcacaaat
     3541 tgaacctgat acaacgcaat tgttggcaac ttttaaagat tccatttttt taaaacaaaa
     3601 tggttttacc aaggaaactg ttttttcaat gtttcttccg aatacctatc aagtttattg
     3661 gaacatttct gcagaaaagt ttagaacgaa aatgctcgac gaatacaaaa aattttggaa
     3721 tccagagcgc attaacaaag ctgaaatttt ggaattaaca ccaattcaag taacaatatt
     3781 agcatcaatt gttcaaaaag aaaccgcaaa aaaatcagaa cgaaaaactg ttgcaggtgt
     3841 ttatctaaac cgattaaaag aaggaatgcc attgcaagca gacccaacag taatctatgc
     3901 tttaaaatat agagacaacg attttaatca aatcataaaa cgagttttgt ataatgattt
     3961 atttataagt tctccttata atacttattt ggtcacaggt ttgcctcctg gaccaattac
     4021 catgcctgat gtggatgcca ttgatgctgt tttgaatgca gaaaaacata attatttata
     4081 tttctgtgct agtacggaga aatttgggta tcatgttttt gcggcaactt acgaacaaca
     4141 tcaagtaaat gctaggaaat atagtgcttg gttgaataaa ttaggtacaa aaagataaaa
     4201 attgagtaat ataaaaagtg ttttatttgt tttacttttt attggattcc ataacgcatg
     4261 gtcacaaaat ggcattgata attatttaaa gccttctgat actcttgata aaaaaagact
     4321 caatgctgta attgtttctg aaacagtagt agcttctgcc actttgattg gtctaaatca
     4381 actttggtat gcagattatc caaaatcaaa ttttcatttt attaatgata atgaggaatg
     4441 gttgcaaata gacaaagctg ggcatctttt ttcttcttat catctaggta aaatcggagc
     4501 tgatgctcta aaatggtctg gtgtttccaa aaaaaatcaa ttgatttatg gttcctctct
     4561 aggattagca tttttaacta ctgttgaagt ttttgatggt ttttccgcta attggggtgc
     4621 gtcatttggc gatgttgtgg ctaatgtttc tggtacagca ttatatattt ctcaagaatt
     4681 gctttggaag gagcaacgag ttataccaaa attctctttt catacaactc gatatgcttc
     4741 actaaggcca gatgttttgg gaagttcatt aaatgagcaa attttaaaag attataacgg
     4801 ccaaacttat tggttgtcat gtaatattca ttcttttttt aaagaatcta aaattccaaa
     4861 atggttaaat gtcgcttttg gctatggagc tgaaggaatg attactggcg acgaagatac
     4921 aggaaatgct atttttattt caaaagatga aagatataga caattttatc tcagttttga
     4981 cgttgatttg actaaaattg aaaccaaatc tcatgtttta aagacattat tttctgtttt
     5041 taattcactc aaaattcctg ctccaacgct tgaaatcaat ggttcacatg gtgttaaggt
     5101 tcacttattt tacttttaat tgactgattg tcattgcaat gaaatatttt gtatatttgc
     5161 actcagaaat atcaagatag tgacagtgct tggaaataaa gatgaatgat acaaaaaagt
     5221 atattttaca cttcaatttt gctaatagtt acttttatta gttttgggtt taagccctat
     5281 gataatcaaa ttaatcaatg gtttgatttc gattcttccg atgaagcttt atatattttt
     5341 ccttctcaac aaaacacgga ttacacttat ttgaatgcac cttttacggg caaatttttt
     5401 ataggttata aagaagctat tgggtttaaa gaatctcaaa ataaatacca aaaagtaaat
     5461 actttgggtt atatgggaaa ataccaattt ggtatggaaa ccttaaaatc aattggcgtt
     5521 tctgacagtg cacgatttat aaacaatcct aaattacaag aaaaagcatt tgttacattg
     5581 ttgtctagaa acaaatttat tcttcaaaat tatattgcta aatacgaagg aaaagtaatt
     5641 tctaatatca aaatcactga atcagggatt ttagcagcag ctcatcttgg tggagcaggt
     5701 tcggtaatta agttttttaa atcaaaagga gaaagacgtg ttaaagacga atatggaacc
     5761 tctatccgtt cgtatttaaa ggaatttgca ggatatgaaa ctaaaaatat tccagctatt
     5821 aagaacgcta ggataaaata aactacattt tgtgaataat aaaaattgta ggtcgattgt
     5881 gtaaatcgac ctttattttt ttccaatctg ccactcgata cgttttgata tattccgaag
     5941 gtaaagtaat gtcgcaagca atgcaaagtg aggtattggg ttgaagaaat tgcaataaat
     6001 cttccattaa tttattgttt ctataaggtg tttcaataaa aagttgcgat tgattttttg
     6061 aatgcgatag tgtttcaaag tttttgatag catttttttt ctcacttttg tcaataggta
     6121 agtaaccatt aaaagcaaaa ctttgaccat tcataccact tgccatcatt gctaatagga
     6181 ttgaggatgg gccaactaaa ggcaccactt gaattccatg ttcatgagca atttttacaa
     6241 tggcagcacc tggatcggca acacccgggc aaccagcttc actcattaga ccaatgtttt
     6301 ctccatttaa acaaggttta atcatggatt gtaattctga atcttcggtt cttttattga
     6361 gtaaagaaag ctttaaatcc gcctgaattt ttgatggttg aactgacttt ataaatttac
     6421 gtgctgtttt ttcgttttca acaatgtagg tggtgagtaa ttccacggtt ctataaacca
     6481 aacttggcaa tacttcatct ggattcattt ctcccattgt ggttggaatc aagtataatt
     6541 tgccttttgg attcatgaat gtttttcgat taatttcttt gctaaaattg cgcaggtttc
     6601 gtctagcatt tcatacacca tgtcaaaacc attttgtaat ccataataag gatcgggtac
     6661 atcaacatta tctcctggaa ataagtgatt taaaattaaa tctacttttt ctttttgttc
     6721 ttctgttttg gctaattcta tgatgtcgtt atagttggag ttgtccatag ctagtatata
     6781 atcaaaatta tcaaaatctc tagaagagaa ttgtcttcca cgttggttgg taatatctaa
     6841 gccgtttttt tgagcaatag caatagagcg agaatctggt tgtttaccaa tgtgataatt
     6901 tccagttccg gcagaatcga ccataaattt atctttagga agtttagagg ctaatattcc
     6961 ttctgccaat ggtgaacgac aaatattgcc caaacaaacc attaaaattt taacagccat
     7021 attcaacttg atttaaaaaa agcgagtaaa aaaactcgct tttataagga tagtttttta
     7081 tgtatatcat ctacaaactt cttaaactgt ttgtcggtag aaactaagtt gtcaacagtt
     7141 ttacaagcat gtaatacggt tgcgtgatct ctgtctccaa tttgagatcc gatattagct
     7201 aaggatgctt ttgtaaattt cttagcaaag aacatggcta attgacgggc ttgaacgaca
     7261 tgacgttttc ttgtttttga ttgcaatgtg tccaaatcca attggaaata atctgaaacc
     7321 actttttgaa tgtagtcaat agaaatttca cgtttgatat ttttgacaaa tttttcaaca
     7381 atacttttag ccaaatcaat cgtaacttct tttttattaa aagaggattg tgcaattaat
     7441 gaaataatag caccttctaa ttcacgaaca ttcgttttta tatttcgggc aacatattca
     7501 atgatttctt caggcatttc aacaccatca cgatacaaga tgtttttcag aattgaaatt
     7561 cttgtttcgt aatcgggctg atgtaattcc gcagataaac cccatttgaa acgagataac
     7621 aaacgttgtt ctatatcttg catgtcaaca ggtgctttgt ccgaggtaag aattacttgt
     7681 tttccgtttt ggtgtaagta attgaaaata tggaagaaaa catcttgtgt tcctgatttt
     7741 cctgaaagaa attgaacgtc gtcaataatt aacacatcta ttaattgata gaaatgaata
     7801 aaatcgtttc tattgttctt ttttacagaa tctatatatt gttgtgtaaa aacttctgct
     7861 gaaatatata atactgtttt ttcaggatat ttgtctttaa tttcaacacc aatagcatgt
     7921 gctaaatgtg ttttccctaa accaacaccg ccgaaaatca ataatggatt aaacgaagtt
     7981 ccaccaggtt tattagcaac agccattcca gcagaacgag ccaatctgtt agaatcacct
     8041 tctaagaaat tgtcgaaact ataattagga tttaattgcg attcgatttt taaatttcta
     8101 atgccaggaa ttacaaatgg attttttaat tcggtgctaa tgttcttata gggagcatca
     8161 acatcttgag atttaatcgg gcttctatgt gcacttggta actgttccgt aaaaggttgt
     8221 ttattaccat aagtgttttc cattttgatt ttataaagta actttgcatt tttgccaagt
     8281 tcttttgtta atgctacttt taataacttt acataatgtt cttcaagcca ttcgtaaaag
     8341 aatttgctag gaacttgaat gtaaagtgca ttatctgtaa gttcaactga ttttatcggt
     8401 tcaaaccaag ttttatacgc ttgctcttga atgttatctt taataaaaga tagacagttt
     8461 tcccataccg attgcgcagt tttgctcata aattctttaa ttatagtata tttgttaggt
     8521 tatgtttaca aaaaaaatca atcaaatttt ctttgttttt cggggtaaca aatatgtgaa
     8581 taaatttcgt taaaaaaaat taaatcacca ttgatttttt gaaaatattg ttgtattgag
     8641 tgtttctaaa tagataaatt aaatataaat taaaatgaaa gaacatcaag ttcagttaag
     8701 agtgaggtat tcagaaaccg atcaaatggg cgtggtttat catggtagct atgtgcctta
     8761 ttttgagata ggtcgtgtgg aatggctaag aaacaaaggg gtgtcgtata aagagttgga
     8821 agaaagtggc gttgctttgc caattgtttc gatgacatta aactataaaa aaccagcacg
     8881 gtatgatgat ttacttacag taattactaa attcaaaagt caatcctctg tgaagataga
     8941 atttgattgt gaaattcgca atgaaaataa tgaattgtta acaactgcac attttatatt
     9001 ggtttttgta gatataaaat taggaaaagc tattgcgccg ccgaaggcta ttttggaatt
     9061 gatgaaagag ggttaattta aagttgtgtt acttcaattt caaacaaatt attaaaaatg
     9121 tcgaaaatca tttcggcatt tttttttctt gttgaaatga ttatttcgca actttcattc
     9181 atggtttgtg aaacaatatc taaatttttc tctttaatta ctcgcattac tttattgatg
     9241 tttttgtaat caaaagaaat tttatactca atatcaatag tttttttaat aatttcagcg
     9301 gtttctaaag tcatttgtgc tgaagttttg taagcagaaa ttaagcctcc aacacctaat
     9361 ttagttccgc caaaatatct aatgatgaca attagaatat tagttacatc aaaagattga
     9421 atttgtccat atattggcat tccagcagaa ttattgggtt cgccatcatc attaacacga
     9481 tactgttttt cttcggtgcc aatttgatag gcataacaga aatgtcgtgc actattatgc
     9541 tctttcttta aatcatccaa acagttttta acgtcttctt cggaagtaac tggaaaagca
     9601 tagccaaaaa atttactatt tttttcttta aataggattt cagaagatgg acaggcaatc
     9661 gtcaaataag aatctttcat aatcaaaatc aaattcttgg gcaataatta gaattgttgg
     9721 aatttggaat ttaacttttg taattttata aaggaaattc ttttttttca aacaaatcaa
     9781 ctacatcttc ttttccaact tgtaggttcc aaacgtataa tcccaaagct gcagcagcat
     9841 cagtgttttc ttttttgtca tcaacaaaaa gtgttcggct cggattaaga ttgtgtttac
     9901 ttactatcgt agtgaaaatt tcaggttgtg gttttcgcat tcccatttcg tatgaataat
     9961 atactttctc aaaacaacga tagaaatcag agtagaaaga catacaaaca ttttcttcaa
    10021 aacaattaat atgaatagca tctgtgtttg taagtaaaaa caagcggtat tttgtagaga
    10081 gtaattgtaa aaattctaaa cgatgtaatg gaaactctcc aattatagaa ttccatgcgg
    10141 aaattatttc ttcactcgtt gcttcgggaa tgtatttttt gaaacaattg ataaattctg
    10201 tttctgtgat tttacccttt tcaaacagat cattgtgcgc tattaaatct tcatttggac
    10261 cgttaagacc taatttttta aattcgtcaa tcgatttttg tttttccaaa ttgataaaaa
    10321 catcgccaaa gtcaaaaata attgcttcaa tcatggttgt aaaataaggt taggttatcg
    10381 tttttaattt tacttaaagt agaataatga tttaaaacgg gtgctttaac accatttttc
    10441 atttccaaat tgcttttaaa aactcttgct tcatcccaaa gactggcatt tataaatgtt
    10501 tctaaagtaa tagttccacc ttcgataatt actgattgga ggttatgttt ccataaaata
    10561 actccaattt tttcagcaag ttgagtatca aagatagtat tttcataaat acaattttca
    10621 gttgactgta aattttcttg ttctgttagg ataatcgttt tcatttgatt gtctaaaaca
    10681 aaataatctg aagttatttt tccagattta tctagtacta ttctaacggg atttttacca
    10741 tgccaattac gaacgtttaa ttttggatta tcttcaagaa cagtgtttgt tcctgctaaa
    10801 atagcttgtt cttcgcttcg ccatttgtgt actaattgtc ttgaaaattc attcgttaac
    10861 caaaagggtt cttttttctc tttagttaat ggcgctatgt atccatcggc actttcagcc
    10921 cattttaaaa taatgtaagg tctttttttg ttttgaaatg taaaaaaacg tttgttcaag
    10981 tcataacatt ctttctccaa aacaccgacg gttattttaa tatcgttttc aattaatttt
    11041 ttaattccat ttccagccac tttcggattt ggatcaatag ttcctataac tacatttgga
    11101 attttatgtt tcataattaa atcagcacac ggtggtgttt ttccaaaatg actgcatggt
    11161 tcaagcgaga cataaatagt agcttttgat aacaatgatt tatctttaac cgaattgatt
    11221 gcattgactt cagcatgtgc ttcgccagct tttttatgcc aaccttcgcc aataattttt
    11281 ccgttgtaaa caatgacaga accaaccaat ggatttggat aggttgttcc caaaccattt
    11341 ttagctaatt gaatgcagcg ttttatgtaa atttcatgta ttttcacatc acaaaaataa
    11401 actattacaa cgataatgag taaaatcgta attagagaaa ttcaatctaa agacaaccag
    11461 caaattgcag ctgttattag agaagttttt attaatgata attatccaaa aactggaact
    11521 gcatttgctg ataagcaatt ggattttatg tttgaaactt acgataaaga aaaggcaatt
    11581 tattttgtaa tagaaaatga tggaaaaata attggtggcg caggaatttc tcaacttgat
    11641 aatgcggatt caaacatctg tgaattgcag aaaatgtatt tcctaaacga agctcgtgga
    11701 aaaggattgg ggtttaaaat tattcaaatg tgtttagaaa aagcaaaaga attcggcttt
    11761 gaaaaatgct atttagaaac tttgcctgag atgaaaacag ctcaacattt gtatcaaaaa
    11821 tcaggttttg aatatattga tgcaccaatg ggaaatactt ttcataatag ttgtcctgtt
    11881 tggatgataa aagatttaag caaataaaat gttactcaaa aaatataaat ctaatttcat
    11941 tgaagaactt tcatccattt acgatgaaat ggaagcagag agttttttct atttaatttt
    12001 agaacattta catcaattca aaaggattga tttagcacta aatccagatt ttgaaatttc
    12061 agagagtgat aaggttagat gggagaatat actttctgaa ttaaaaactc aaaaaccgat
    12121 tcaatacata ttaggcgaaa ctgaatttta tggattgaaa tttatagttg atgaaaatgt
    12181 tttaattcca agacctgaaa ctgaagaatt ggttgatttg atagtttcaa gttttaaatc
    12241 acaaacttca aagttgagaa ttttggatat tggaactggt tcgggttgta ttcctatttc
    12301 gttgaaaaag aatttaccaa atgctgaagt ttttgccatt gatgtctctg aaaatgcatt
    12361 agaaattgcc aagaaaaacg caaagttgaa tggagtagaa gtgaacttca ttctaaaaaa
    12421 catattggaa acaaatgatt tagaacaaca atttgatatt atcgtttcaa atccgccgta
    12481 tgttagaatg ttggaaaaag aagaaattaa aacgaatgtt ttagaatatg agccacattt
    12541 agcacttttt gtagaagata atgatgcctt aattttttat agaaaaattg ctcaactcgc
    12601 caagaaaagt ttaacagaaa acggtaagtt atacttcgaa atcaatcaat atttaggaaa
    12661 agaaaccgtt attctacttg aaaaaatgga tttcaaaaac attgagttat tgcaagatat
    12721 ctatggtaac gatagaatga taagttgtag taaaactaat aattattcgt agcgcaatgc
    12781 ttcaattggg tctaatcttg atgcttttaa agctggataa gagcccgaaa ccactgctac
    12841 aataaaggtt gtcaaaacag cagccataat tgccaaccaa ggtatcacaa aagaaaaatc
    12901 aatggctacc gctatcaaat aaccagtagt aatcccaagt agaattccaa ccaatccacc
    12961 caattgtcca atgattaatg tttcgacaaa aaattgaaaa gcgatagtat ttcgtttggc
    13021 accaagtgct tttctaactc caatctcacg tgttctttcg gttacagaaa cgagcataat
    13081 gttcatcaat gcaatagaag aaccaaaaat agtgatgatg ccaattaccc aagccgaaat
    13141 gtttaacaca ccagtcattt cggcaattct gttgattaaa tcatcacttc ttgaaatacc
    13201 aaaattattg tcttctactg gatttagctt acgcacttgt cgcatcgtaa ccaatgcttc
    13261 gttaatagct gcatcaagaa actctttttg ttgcaccatc gaactaatgg tgtaatttat
    13321 gtttggagcc gaaaacatgg aacgtgctac ttgaataggt atcatcattc gcaaatcttg
    13381 actgtttcca aaggtggaac ctttctcttt taaaacgcca attactttaa attttgcacc
    13441 acgaaccgaa agtattttac caatcggatt gatgtctttt agcaaacctt ttgtggcaaa
    13501 atcggaaccg acaacgcagc tataggtgtt gttatcaatg tcaaatttat tgaaatttct
    13561 tccttgcgta acttctaatc cagagtttgg tatatagaat tcatccacgc caactacaga
    13621 aatttcagga tctgttttct cgttttcaaa tttcacttcg gccgttgagg tagcataaaa
    13681 tgaaatagaa gtattggttg aaggaaaatc aaaatgctct ttaaaagcct tggcttccgg
    13741 ataggaaatg attggattaa ctttttgaac ttcatcgcca ccatttcttc tagcggtaaa
    13801 ttcatagcga ttgatattaa aagtgttcga acccattgat gcaaaattgg ccgatagtgt
    13861 gttttctagc gcagaaacta cggttagaat accaactaac gcccaaattc ctaaagcgat
    13921 aattagaata gtcaatatcg ttctcaataa ttgagtgcga attgaaccaa gtgcaatttt
    13981 tatattttct aagaaaagtt taaacataac atcaatttga cactaaaata cagtttttgt
    14041 tacaagttta tcctaaatca attttaaaaa ggagtaaaaa ataagatatt tgcactgtca
    14101 atatcagatg ttgcatagta ggtttcaatt tttgaagtct attttctatc atctttaaat
    14161 ctattatcta aaataaaatg gcacaaaaac caagtattcc caaaggaaca agagattttt
    14221 caccggttga ggtttcaaaa cgtaactata ttattgctgt aatgcgtaag cattttgaaa
    14281 actatggtta tcaacctatt gaaacaccaa gttttgaaaa ctctgatacc ttgatgggaa
    14341 aatatggtga agaaggcgac cgtttgattt ttaagatatt gaattctggt gattattttg
    14401 aagatgtcag aaaggatgtg ttttattctt caaatattaa tgctgttttt tttgatattt
    14461 tcgataatct tgtgcagttt gacgtaaata aatcagataa gtataattta tttttgataa
    14521 agtttctaga tgtattttct aaaaatagaa ttacgtcatc aaatgaaaag aatgaaagaa
    14581 gtttgattga aaaagtattt ttggagaata aggataagat tcttgaattt gattatactg
    14641 acaagcctaa tcttgctaat ttcaggagac aaatttttac agtttttttt aaagaataca
    14701 ttgaagagtt tgatgtgtta aattctcaga aaggaacaaa aaaaatctcc gaaaaagcac
    14761 tccgttacga ccttaccgta ccttttgcta gatacgtggt gcaacaccaa aacgaaattg
    14821 agtttccgtt taagcgttat caaattcaac cggtttggcg cgctgataga ccacaaaaag
    14881 gacgtttcag agaattttat caatgcgatg ccgatgttgt aggttcaacc tcgctttggc
    14941 aagaagtaga gttggtgcaa ttatacgatg ttgtttttaa tgatttaggt ttgaatggcg
    15001 ttactatcaa aatcaataat cgtaaaatac tatcgggaat tgctgaggta attggtgctt
    15061 cggataagtt gattgatttt acagtggcac tagataagct agacaaaata ggcgaggaag
    15121 gcgttaaaaa agaaatgctc gaaaaaggaa tatccgaaac agctttagaa aaagtgcagc
    15181 cattatttaa cttcactgga actattgatg aaaaattagc aaaactttcg gcattacttt
    15241 ctaattcaga agaaggaaaa aaaggcgtag aagagttgcg ctttatttgt gaaaacgtgt
    15301 ccaaattagg tttgcagcaa gccaatttag acttagatgt aaccttagct cgcggattaa
    15361 attattatac aggagccatt tttgaagtag cagcacccaa agaagtagcc atgggttcta
    15421 taggcggcgg cggaagatat gatgatttga caggaatttt tggtctaaaa aacatgagtg
    15481 gcgtgggtat ttcttttggt ctcgatagaa tctatttggt tttggaagaa ctaaatttat
    15541 ttccagaaac agttaccaat actacgcagg ttttgttttt aaattatggt gaaaaagagg
    15601 cttttgaagc tatgaaaacc atcactacac taagaaaaga aaacatcaaa gccgagttgt
    15661 acccagatgc ttcaaaaata gacaaacaat ttaaacacgc cgaacgccgg ggaattcctt
    15721 ttgtggtaaa agaaatagaa ggcaacaacg ttacgttgaa agatttaaaa acaggcgaac
    15781 aattggtgct agatgtaaca catcttatca aaaaactttc ataattacat gctacgctcg
    15841 tagggtatat gtctaaacgc tttaggatgc tatgctacgc tcgtagggta tatgtctaaa
    15901 cgctttagga tgctatgcta cgctcatagg atatatgtct aaacgcttta ggatactatg
    15961 ctacgctcgt agggtatatg tctaaacgct ttaggatact atgctacgct catagggtat
    16021 aggaaataaa aaaactccct tgtgtaggag ggagtttttc agatttgggg aaatctttga
    16081 aatttggggg gaatcttttc gtcaaaaaaa attataaaaa actacttaat taggattttc
    16141 ttgctaatat tacctttgtc ggtaattact tttacgatat aagcacct
//
LOCUS       DAOOBH010000044        15725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_4875_length_15725_cov_4.733950, whole genome shotgun sequence.
ACCESSION   DAOOBH010000044 DAOOBH010000000
VERSION     DAOOBH010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15725
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4875_length_15725_cov_4.733950"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PK218_06175"
     CDS             <1..499
                     /locus_tag="PK218_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HPW98127.1"
                     /translation="LIKMVGGSPLIVKLLEGTQGKGVVLAETKKAAESVINAFKSLNA
                     NILVQEFIKEANGKDLRLFVVDGKVVATIQREAAPGEFRANIHMGGTASIIKPTADEK
                     KIAIKAAKAMDLKVAGVDIIRSSKGPLLLEVNSSPGLEGIEGATNKDIAGEMIKAIEK
                     NFKWK"
     gene            582..1160
                     /locus_tag="PK218_06180"
     CDS             582..1160
                     /locus_tag="PK218_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HPW98128.1"
                     /translation="MNKSRLEAFSDGVLAIIITIMVLEIKVPHGNELSDLHPLIPIVL
                     SYVLSFIYVGIYWNNHHHMMQTVKNINGAILWANLHLLFWLSLIPFATAWNGENHFSN
                     ATMMVYGFVLLMAGIAYFILQNIIIKLQGNDSLLKKAIGKDFKGKSSPIFYLIAIIFA
                     NFLPIISGIIYVLVALMWLVPDKRIEKIINHQ"
     gene            1160..1522
                     /locus_tag="PK218_06185"
     CDS             1160..1522
                     /locus_tag="PK218_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HPW98129.1"
                     /translation="MNLLSQIIIGFIAVLHLYILWLEMFAWTTNAKKVFKTIPPDMFE
                     KTKVMAANQGLYNGFLAAGLLWSLAISDANWSINVALFFLSCVFIAGLYGAATASKRI
                     FFVQSVPALLGLLSIFLS"
     gene            complement(1944..3446)
                     /gene="hutH"
                     /locus_tag="PK218_06190"
     CDS             complement(1944..3446)
                     /gene="hutH"
                     /locus_tag="PK218_06190"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPW98130.1"
                     /translation="MEQSHYISSEVLSLEVIQEIISQNKKLALSDEAKVAIEKCRTYL
                     DTKMASNDEPIYGINTGFGSLCNVKISSENLSQLQENLVKSHACGTGEAVPQEIVKIM
                     LLLKIQSLSYGHSGVQLQTVDRLIDFYNNDILPIIYTQGSLGASGDLAPLAHLSLPLL
                     GEGEVYYDGFRQPASKVLEKMNWKPIILQSKEGLALLNGTQFMSAYGSYILLKAMKFS
                     YFADVIGAISLEAFDGRKEPFNPLIHYIRPHKGQIVTAERILEFLEGSQIIQQPKQHV
                     QDPYSFRCMPQVHGASKDTIDYVKKVFKTEINSVTDNPNIFIESDIIVSGGNFHGQPL
                     ALALDFLSIALAELGSISERRTYQLISGLRGLPAFLVDNPGLNSGFMIPQYTAASIVS
                     QNKQLATPASIDSIVSSNGQEDHVSMGANAATKCLKIMENLERILAIELMNASQAIEF
                     RRPLQSSEFIETFLKSYRDEVPFVKEDRILHYDIEKSIAFLNSFMVELDN"
     gene            3883..6330
                     /gene="thrA"
                     /locus_tag="PK218_06195"
     CDS             3883..6330
                     /gene="thrA"
                     /locus_tag="PK218_06195"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HPW98131.1"
                     /translation="MITLKFGGTSVANAENISKTVAIIANKSNEHKLAVVVSAFSGVT
                     DLLILAGTTAAAKNKKYKDIVVEIEQKHKSAIDELIPLTDQSDIIDTINSTINHLKTL
                     LDGCYLLGEISIRTADSVAGFGELLSSQIIAVALKQKVATATFKDSRELIKTNSNFGK
                     ASVDFAKTNQLIANYFAANKNQVVLLPGFIASDVAGNTTTLGRGGSDYTAAIIAAAVK
                     ADVLEIWTDVNGMFTANPKSVKQAQPIEHISYQEAMELSHFGAKVLYPPTIQPVLKDS
                     IPIFIKNTFEPSAYGTLISNNPENNTNPIKGISHIDNIALLTLEGSGMIGVSGSSKRL
                     FETLSRENINVIFITQASSEHSICIGILNADADNAKAAIDAMFEIEIAQNKIDPCVVE
                     KDLCIVALVGESMKNHQGISGKMFSTLGKNNVNIRAIAQGASERNISVVINEKDVKKA
                     LNTLHERFFEDNTKQLNLFVMGIGNVGEKFIDQIAQQKKFLKENLKINLRVIALSNSR
                     KMYFNEDGISLKDWKNEVDAGETANVNTFIARAKTLNLRNSIFVDITANYDIAGIYDQ
                     FLCENIAVVTCNKIACSSQYENYKNLKGLSRKFNAPFLFETNVGAGLPIIDTLKHLIA
                     SGDKVNKINAVLSGSLNFIFNNFSDTYSFHDVVKEAGVQGFTEPDPKIDLSGVDVARK
                     ILILIRESGYQMEMEDIENNCFLPQECMDTKDNASFFASLIKHKDHFNKLLAEAKAKN
                     SRIKFVATFDNGKASVGLQFIPQDSPFYNLEGKDNIVEFYTDRYVEQPLIIKGAGAGA
                     AVTASGIFADVIRIGNV"
     gene            6382..7308
                     /locus_tag="PK218_06200"
     CDS             6382..7308
                     /locus_tag="PK218_06200"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPW98132.1"
                     /translation="MNEIKIFAPATIANLNCGFDVLGLCLDGIGDEMIFRKSTEKGLR
                     ITKITGADLPLELENNVAGVAGLALLDKVEIDFGIEIEIYKKLKLGSGIGSSSASAAG
                     AVFGINELLGKPFTKKELVNFAMKGEAVASGSEHADNVAPCLLGGFTLVTGYNPLEVI
                     RIEAPADLFVVVLHPHIEVKTSDARAVLKPEISLKNAITQIGNLGGFVAGLYTNDYDL
                     IGRSMKDVLVEPYRKHLIPNFDLVKNVALSNRALGAGISGAGPSIFALCKGKKTAETV
                     ANAMQNAFKETKIPYDIHVSKINEEGTKLITN"
     gene            7314..8606
                     /gene="thrC"
                     /locus_tag="PK218_06205"
     CDS             7314..8606
                     /gene="thrC"
                     /locus_tag="PK218_06205"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010521109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPW98133.1"
                     /translation="MNYFSLNNKNHKVSFKQAVINGLAPDKGLYFPDNITSLPTTFFE
                     NIENSSNEEIAFEAIKQFVGDEIPEIILKQIIAETLCFDFPCVKVEDNVYSLELFHGP
                     TMAFKDVGARFMSRCMGYFNQNSEEKNTVLVATSGDTGGAVASGFLGVKNVEVIILYP
                     SGKVSDIQERQLTTLGKNIKALEVNGVFDDCQDMVKNAFLDKTLKHKNLTSANSINIA
                     RWLPQMFYIFFAYKQLKKHNKPILLSCPSGNFGNICAGILAKRLGLPIAHFVASTNAN
                     DTVPRFLENGNYEPKPSVVTISNAMDVGNPSNFVRIQELYNHDLAAFEKDFSSYSFTD
                     AETEFAIKDIYYRTNYIAEPHGAVGYLGLEKEMEKKPDNIGVFLETAHPIKFLDIVEP
                     LLNNKLPIPKQIESVLNKEKVRTKIATYEDLKEFLLRN"
     gene            complement(8603..9415)
                     /locus_tag="PK218_06210"
     CDS             complement(8603..9415)
                     /locus_tag="PK218_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPW98134.1"
                     /translation="MIVLLICISFLISLFGSIVGFGGGIFIVPILIAFFGYPIELAVG
                     STMFSLIPSAMMSTFLNRKESNVDFKMGILLEVPTMIGVILGSLLLSYIAPKNLEIAF
                     SVMVLLIGCSFFIKRNQDNIKLGFFDKLNHLKPRYIIKNRKKHVAYRVSLQMVLFFGM
                     LSGTIAGLFGMGGGFMKTPIMLKVFKIPSKIATATALFMILITSVTGSISHYLQGNIQ
                     IEKTWPVMIGFTIGAFVGHKISTSISSTLLEKLIGIALLLASMIMFFNFLNH"
     gene            9611..10456
                     /locus_tag="PK218_06215"
     CDS             9611..10456
                     /locus_tag="PK218_06215"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPW98135.1"
                     /translation="MKNSVKIIALIVSVIFLSCNEKKESKTHKKPIVERIQPKEISFT
                     FQKSKEWLNGNTDTKKAMNIVLAINRVDKSNLIKLDSIIVPTDISGDIAYYMPFPLDV
                     SSLEEVKKIIVFSYATQAFATYEYGVLTHVGPTSMGKEKDKTPTGLFFTNWKAEETTS
                     TFNDEWKLKWNFNIENKLGVGFHQYELPGYPASHSCLRLLEKDAKMLYTFADEWVLES
                     DQKIELKGTPVIVFGSYDFEGQKPWYDLIENPKALSISKEQLEKVISPYLSTILKEQA
                     KRENK"
     gene            complement(10473..11087)
                     /locus_tag="PK218_06220"
     CDS             complement(10473..11087)
                     /locus_tag="PK218_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW98136.1"
                     /translation="MNLKKINPNLQKALVENGLTQANEMQIETFSTIKSGADAVIQSP
                     KGTGKTTTIVLNVIQRLERTVGESTRALIMVESKEKVLEMEELFLKYANYHDLSILGV
                     HDKGDIDYDKNIISLGLDILIGTPNRINALFSSAGFNASTIRMFVVDDADILFKNRVD
                     AIVLRLSMSIEKTQRLFFCSEIIERVETLADKVMIEPLFFEMEA"
     gene            complement(11184..12347)
                     /locus_tag="PK218_06225"
     CDS             complement(11184..12347)
                     /locus_tag="PK218_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPW98137.1"
                     /translation="MSKILIIEDEAAIRRVLVKILSEESTTYQVDEAEDGKIGFDKIK
                     NDDYDLVLCDIKMPKMNGEELLEAVKKIKPEIPMVMISGHGDLETAVNTMRLGAFDYI
                     SKPPDLNRLLNTVRNALDKKQLVVENKILKKKVSKNYEMVGNSAPINQIKEMIEKVAP
                     TDARVLITGPNGTGKELVAHQLHDKSARSEFPLIEVNCAAIPSELIESELFGHVKGAF
                     TSAVKDRAGKFEAANGGTIFLDEIGDMSLAAQAKVLRALQESLVQRVGADKDIKVDVR
                     VVAATNKDLKKEIEEGRFREDLYHRLAVILIKVPALNDRRDDIPLLIEYFSNKIAQEQ
                     GSVSKSFSKDAIKLLQEYDWTGNIRELRNVVERLIILGGTEISETDVKLFASK"
     gene            12472..13290
                     /gene="panB"
                     /locus_tag="PK218_06230"
     CDS             12472..13290
                     /gene="panB"
                     /locus_tag="PK218_06230"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPW98138.1"
                     /translation="MSTAKKDYKRITTKSLFDMKKNGEKISMLTAYDYTMAKIVDSAG
                     VDVILVGDSASNVMAGHETTLPITLDQMIYHASSVVRAIERSLVVVDLPFGSYQSDPK
                     EALRSAIRIMKESGGHAVKLEGGSEIKESIKRILNAGIPVMGHLGLTPQSIYKFGTYT
                     VRAKEEEEAEKLIEDAKMLERIGCFALVLEKIPASLATKVAQSISIPVIGIGAGGGVD
                     GQVLVIHDMLGMNNEFNPRFLRRYLDLYEQMNKAIGQYVTDVKSKDFPNESEQY"
     gene            13331..14029
                     /locus_tag="PK218_06235"
     CDS             13331..14029
                     /locus_tag="PK218_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HPW98139.1"
                     /translation="MTDKIFTTKDNLQILHEDNHIIVVNKRVGDIVQGDKTGDKPLSE
                     VVKEYIKDKYNKPGEVFLGVVHRLDRPTSGIVVFAKTSKALTRLNELFNKRETQKTYW
                     AIVKNYPPKENDILVHFLKRNPKNNTSKAHIKEVPESKKASLEYTIIKKLDSYYALEI
                     NLHTGRHHQIRAQLQAIGCPIKGDLKYGFDRSNPDGGIHLHARQLVFIHPVAKIEVSL
                     IAPTPNDVIWNAIG"
     gene            14118..15455
                     /locus_tag="PK218_06240"
     CDS             14118..15455
                     /locus_tag="PK218_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98140.1"
                     /translation="MKKYIYSFFLISSTMVFSQDHYSGISTSNRVGILNVTINPAELP
                     NLSKKFEVNIYGMSFNVANNKIGFSDFNSDKDFEELLFQGTSPVNARIDTDIIGPGFA
                     MQLGKWGFAFTTKAYAKFDMVDVDPTIGNAITNNNLVLNTTLLNNPNNQRLSGTSYGE
                     IGLSAGRTFFENDKHKFSAGVTLKILFPGSYSNFGLSQLDGTITQSLGNSYLTTNSPA
                     SLNIAYSGNLANSFSNFDDYSKSIFGGLNGLAGDIGFNYQLKSDTKYKLNAGLSIRNI
                     GSMTFKDENNYNTSYTLNIPSSNPLDLSLFDNIDNLSEVEDILTNNGYLTTQPRNKTD
                     FNVKLPTTVNTYVDVKVVSKLYVTAFLQQKLNKDRDNEQVTTPNIFSITPRVNLGFFE
                     GYIPLTFHEISGTNVGLGFRLGGFYLGTSSMVTALISDSKQGDLYFGFRFGFL"
     gene            15519..>15725
                     /locus_tag="PK218_06245"
     CDS             15519..>15725
                     /locus_tag="PK218_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HPW98141.1"
                     /translation="MLFKTSITYRKKTLLKLLIAIEKHEEEINQALYNDFKKPEFESY
                     LTETYFVLSELKLTIKNINKWAKPK"
BASE COUNT         5404 a         2596 c         2672 g         5053 t
ORIGIN      
        1 tttgattaaa atggtaggcg gctcaccttt gattgtaaaa ttattagaag gaacccaagg
       61 caaaggtgta gtgcttgcag aaactaaaaa agcagccgaa tctgttatca atgccttcaa
      121 gagtttgaat gcaaacatac tggtacaaga gtttataaaa gaagccaacg gaaaagacct
      181 acgccttttt gtagtagatg gaaaagtagt ggcaaccata caacgtgaag cagcaccagg
      241 tgagtttaga gccaatattc acatgggtgg aacggcatct atcattaaac caactgctga
      301 cgagaaaaaa atagccatca aagccgcaaa agcgatggat ttaaaagttg ctggtgtcga
      361 tattatccgt tcttcaaaag gtccgttatt gttggaagtt aactcttcac ctggtttgga
      421 aggaattgaa ggtgctacta ataaagacat tgctggcgaa atgattaaag ctattgagaa
      481 gaatttcaag tggaaataat ttttcagaaa gaagttagaa actaaaagct aggaatttga
      541 aatttataat taaaaatact acataaaaca ccgaagcaat tatgaataaa tctagactcg
      601 aggcctttag tgatggtgtc ttagccatta ttattaccat catggtactt gagataaaag
      661 tgccgcacgg aaacgaatta tcagacttac atccactcat tcctatcgtt ttaagctatg
      721 tgcttagttt tatatatgta ggtatctatt ggaacaacca ccaccacatg atgcaaacgg
      781 taaaaaacat caacggcgca attctttggg caaatctgca tctattgttt tggttatcac
      841 taattccctt tgccacagca tggaatggtg agaatcattt ttcaaatgcc accatgatgg
      901 tgtatggttt tgtactatta atggcgggaa tagcctactt catacttcaa aatatcatca
      961 taaaattgca aggaaacgat tcgctactaa aaaaggctat tggaaaagat tttaaaggca
     1021 aatcatcacc gatattttat cttatagcaa taatttttgc caacttttta cctattattt
     1081 ctggtatcat ttatgtttta gtggcattga tgtggcttgt acccgataaa agaatagaaa
     1141 aaatcatcaa ccatcaataa tgaacctact atcacaaata atcatcggat ttatagctgt
     1201 tttacatcta tatatccttt ggctcgaaat gtttgcttgg actactaatg ccaaaaaagt
     1261 gttcaaaacc attcctcctg atatgtttga gaaaacaaaa gttatggctg ccaatcaagg
     1321 tttatataac ggctttttgg cggcaggatt actatggtca ctagcaatat cagatgctaa
     1381 ttggagcatc aatgtagcct tgttcttttt atcatgtgta tttatagccg gattatatgg
     1441 tgctgctaca gcttctaaaa gaatattttt tgtacaaagt gttcctgctc tattaggttt
     1501 attgagtatc tttttgtcat aataactact tcatcaatct tgctttttta aaagaaaaat
     1561 ataactgatt agtatagtta tataacctgt aagtttttcc aaaataaaac atttatacta
     1621 cagcaataaa ccttgaaaaa atgaaaacca ttgtaccata ctgcagtagt atgccttggg
     1681 aaagtgaaat ccatagcacc ttactaccgc atctgcctcg ggaaagtaaa aaccatagta
     1741 ccctactgca gtagtatgcc ttgggaaagt gaaatccata gcaccttact accgcatctg
     1801 ccttgggaaa gtaaagacca tagcactata ctacagtaat atgccttggt aaagtaaaaa
     1861 ccatagcact atactataat agtgtgtata aaaataatat ggtacaaatg cgttaaaaga
     1921 agaatgtaat taatttaaaa aaattaatta tcaagctcaa ccataaaact atttaaaaaa
     1981 gcaatagatt tttcaatatc atagtgtaga attctatctt cctttacaaa aggaacttca
     2041 tctctatatg attttaagaa agtttcaata aattcactcg attgtaatgg tcgtctaaat
     2101 tcaattgctt gcgatgcatt cattaactca atggcaagta ttcgctctaa attttccata
     2161 atcttcaagc acttagttgc agcatttgca cccatagaaa catggtcttc ctgaccatta
     2221 cttgatacaa tactatcaat acttgctggt gttgctagtt gtttgttttg actaacaata
     2281 cttgctgcag tatattgtgg aatcataaaa ccagaattca aaccaggatt gtcaaccaaa
     2341 aacgcaggca aacctcttaa accagaaatt aattgatagg ttctacgttc cgaaatactt
     2401 cctaattctg ccaaagcaat actcaaaaag tctaaagcca aagctaaagg ttgcccatgg
     2461 aaatttcctc ctgaaacaat aatatcgctt tcaataaata tatttggatt atcagtaact
     2521 gagttgattt cggttttgaa aactttcttt acataatcaa ttgtatcttt acttgcgcca
     2581 tgtacttgtg gcatacatct aaaggagtat ggatcttgta cgtgttgctt aggttgctgt
     2641 atgatttggc ttccttctaa aaattctaag atacgttccg ctgttactat ctgtcctttg
     2701 tgtggtctta tataatgtat caatgggttg aagggttctt ttcttccgtc gaaggcttca
     2761 agcgatatgg caccaattac atcggcaaaa taggagaatt tcatcgcctt taggaggatg
     2821 taacttccat aggcactcat aaattgtgtt ccgtttagca atgccaatcc ttcttttgat
     2881 tgtaagatta ttggtttcca attcattttt tctaaaacct tgcttgctgg ctgtctgaaa
     2941 ccatcataat atacttcgcc ttcacccaat aatggtagcg ataagtgtgc taatggtgcc
     3001 aaatcgccac tggcacccag tgagccttgt gtataaataa ttggaagaat atcattatta
     3061 tagaagtcta tcaatcggtc aacggtttgc aattgcacac ctgagtgacc ataacttaat
     3121 gattgaattt ttagcaatag catgatttta actatctctt gtggcactgc ttcgcctgta
     3181 ccacaagcgt gtgatttaac taaattttct tgcaactgcg aaagattttc ggaagatatc
     3241 ttcacattac acaacgaacc aaatcctgtg ttgataccat atattggttc atcgttagat
     3301 gccattttag tgtctaagta ggttctacat ttttcgatgg ctacttttgc ctcatcggat
     3361 agggctaatt ttttattttg cgaaattatc tcctgtatga cttctaatga aagtacttct
     3421 gaactaatat aatgcgattg ttccatgatt gggttgtgtt ttatgcgcca aaattcaaca
     3481 aacaattgct aataagcaaa agatttaggc tcattttatt gacaaattag caatttatca
     3541 attataaacc ataaatagta taaaatctat accactaaaa cgatataact atgaagaatt
     3601 cacaaaagta acaggtaagt ataggtattt aaatgaaaaa gtatatattt gaaaacatat
     3661 gtgcaaaact ttaacaaata ctacatcaat aaagctatcg gctactgctg tattttttgt
     3721 attgggcacc actactacta caattacaac cccatttggg gtataatttt cacatataat
     3781 cctctttatg gactttcaat tgaaagtaag taaattatat tacctattga aaattttatt
     3841 tttatacctt gaaccattta ctcttaaacg cataacatca ttatgattac tctaaaattt
     3901 ggcggaacat ctgttgcaaa tgccgaaaac atatcgaaga ctgttgccat cattgccaac
     3961 aaaagtaacg aacacaaact tgccgtagtg gtttctgcct ttagtggcgt aacggatttg
     4021 ttgatacttg ctggtactac tgccgctgca aaaaacaaaa aatacaaaga cattgttgtt
     4081 gaaatagaac aaaaacacaa atcggctatt gatgaattga ttcctttgac cgaccaaagt
     4141 gacattatag ataccattaa tagcaccatc aatcatttga aaacactgtt agacggttgc
     4201 tatttattag gcgaaatttc gatacgaaca gcagatagtg tggctggttt tggcgaatta
     4261 ttatcgtctc aaatcattgc tgtagcctta aaacaaaaag tagctacggc tacctttaaa
     4321 gacagtcgcg aattaataaa aaccaattct aactttggta aagccagtgt agattttgca
     4381 aaaacaaatc aattgattgc aaattacttt gctgccaata aaaatcaagt ggtattgcta
     4441 cctggtttta tagcttcgga tgtagcagga aacactacaa ctttaggtcg tggtggttct
     4501 gattatacgg cagcaattat tgctgcagcc gttaaagccg atgttttaga aatttggaca
     4561 gatgtgaatg gtatgtttac tgccaatccg aaatcggtaa aacaggcgca acctattgag
     4621 catatatcgt atcaagaagc gatggagttg tctcattttg gagcaaaggt attgtatccg
     4681 ccaactattc agccagtgtt aaaagatagt attccaattt tcatcaagaa tacctttgaa
     4741 ccttcagctt atggaacgtt aatttccaat aatccagaga ataacaccaa tcctataaaa
     4801 ggaatttcac atattgataa tattgcctta ttaacccttg aaggttcggg tatgataggg
     4861 gtttcgggtt cctcaaagcg attgtttgaa acactgtcta gagaaaacat caacgtaata
     4921 tttattacgc aagcctcatc agaacattct atatgtatag gaattttgaa tgcagatgcg
     4981 gataatgcta aagcagccat tgatgctatg tttgaaattg aaattgcaca aaacaaaata
     5041 gacccatgcg tggttgaaaa agacttgtgt attgtagcat tagtaggtga aagcatgaaa
     5101 aatcatcaag gtatttcagg taaaatgttt agtacgctgg ggaaaaacaa tgtgaatata
     5161 cgtgccattg cacaaggtgc ttctgaacga aatatatcgg tagtgattaa tgaaaaggat
     5221 gttaaaaaag cactaaatac actccacgaa cgtttctttg aagacaatac aaaacaacta
     5281 aatctattcg tcatgggtat tggaaacgtt ggagagaagt ttattgatca aatagcccag
     5341 caaaagaaat ttttaaagga aaacttaaag ataaacctaa gagttattgc tctatccaat
     5401 tcaagaaaga tgtactttaa tgaagatggc atttctttaa aagactggaa gaatgaggta
     5461 gatgctggag aaactgcaaa tgtcaatacg tttatcgcta gagctaaaac actcaattta
     5521 cggaatagta tttttgttga tattacggct aattatgata ttgctggtat ttatgatcaa
     5581 ttcttatgtg agaatattgc cgtagtaacc tgcaataaga tagcttgttc atcgcaatat
     5641 gagaactata aaaaccttaa aggtttatca cgaaagttta acgctccgtt tttatttgaa
     5701 actaatgttg gagcaggttt accaatcatt gatacactaa aacatttgat tgcatcaggt
     5761 gataaagtga ataaaatcaa tgcggtacta tctggaagtt tgaattttat attcaataac
     5821 tttagtgata cttatagctt tcatgatgta gtaaaagaag caggtgttca aggttttact
     5881 gaacccgacc caaagataga tttgagtggt gttgacgtgg cacgtaagat actaatctta
     5941 attagagaaa gtggttatca gatggaaatg gaagacattg aaaacaattg tttcctccca
     6001 caagaatgta tggatacgaa agataatgca tctttttttg cttcattaat taagcataaa
     6061 gatcatttca acaagttatt agccgaagct aaagcaaaaa atagtcgcat caagtttgtg
     6121 gctacattcg acaacggaaa agcatccgtc ggattacagt ttattccaca agacagtcct
     6181 ttttataatc ttgaaggaaa agataatatt gtagaattct ataccgatag atatgtagaa
     6241 caaccactaa ttatcaaagg tgctggtgca ggtgctgcag ttacggcatc tggtatattt
     6301 gctgatgtaa ttagaattgg gaatgtgtaa ccttagttag agacgtagca atgcaacgtc
     6361 tggacggtaa aaaacaaaaa aatgaacgaa ataaaaatat ttgcaccagc aactatagcc
     6421 aatctaaact gcggttttga tgtattagga ttgtgtttgg atggtattgg agatgaaatg
     6481 atattccgaa aatctaccga aaaaggactg cgtattacta aaattactgg tgctgattta
     6541 cctttagagt tagagaataa tgtagctggt gttgcaggtt tagctttgtt agataaagta
     6601 gaaattgatt ttggaattga aattgaaatc tacaaaaagc taaaacttgg tagtggcata
     6661 ggtagttcat cagctagtgc tgcaggtgcg gtttttggaa tcaatgaatt gttaggcaaa
     6721 ccctttacaa agaaagaatt agtaaacttt gctatgaaag gtgaagctgt agcttctggt
     6781 agtgaacatg cggacaacgt tgctccttgc ctactcggtg gatttacttt agttacaggc
     6841 tacaatccat tggaagtcat cagaattgaa gctccagctg atttgtttgt tgttgtttta
     6901 catccacata ttgaggtaaa aacttccgat gcacgcgcgg tattaaaacc tgaaatatcc
     6961 ttgaaaaatg cgattacgca aataggaaat cttggaggtt ttgtggcggg tttatacacg
     7021 aatgattatg atttaatcgg tcgttctatg aaagatgttt tggtagaacc ctatcgcaaa
     7081 catttaattc ctaattttga tttggtaaag aatgtggcat taagcaatag agcgttaggt
     7141 gctggaattt ctggtgctgg gccaagtatc tttgcacttt gcaaaggaaa aaaaaccgcc
     7201 gaaacagtag caaatgctat gcaaaatgct ttcaaagaaa ctaaaatacc gtatgatatt
     7261 catgtttcaa aaataaatga agaaggaacg aaattaatta cgaattagca attatgaatt
     7321 attttagctt aaacaataaa aaccacaaag tatctttcaa acaagctgta atcaatggac
     7381 tagcaccaga taaaggatta tattttccag ataacattac atcattgccg acaacttttt
     7441 ttgagaatat tgaaaatagt tccaatgaag aaattgcttt tgaagcgata aaacaattcg
     7501 tcggtgatga aattcctgaa atcattttaa aacaaatcat cgccgaaacc ttgtgttttg
     7561 attttccttg tgtaaaagtg gaagataatg tttattcctt ggaacttttt catggtccaa
     7621 caatggcatt taaagatgtt ggtgcccgat ttatgtcgcg ttgtatggga tatttcaatc
     7681 aaaactccga agaaaagaat actgttttag ttgccacttc gggtgatact ggtggagcag
     7741 ttgctagtgg ctttcttggt gtaaaaaacg tggaagtaat tatactttac ccatcaggaa
     7801 aagttagcga tattcaggaa cgacagttaa ccacgttagg caaaaacatc aaggcattgg
     7861 aagttaatgg cgtttttgat gattgccaag acatggtgaa aaatgctttt ttagacaaaa
     7921 cattaaaaca taaaaacttg acttctgcca attctattaa cattgcacgt tggttgccac
     7981 agatgtttta tattttcttt gcctacaaac aactaaagaa acacaacaaa ccaatactac
     8041 tttcatgtcc aagtggaaat tttggcaata tttgcgctgg aattttggca aaacgattgg
     8101 gtttaccgat agcacatttt gttgcttcca ccaatgctaa tgatactgtg ccgcgttttc
     8161 tagaaaacgg aaactatgaa ccaaaaccat ctgttgtaac aatctcaaat gctatggatg
     8221 ttggcaaccc aagtaatttt gtgcgtattc aagagttgta taatcacgat ttggctgcat
     8281 ttgaaaaaga cttttcttcc tacagtttta ctgatgctga aactgaattt gcaataaaag
     8341 atatttatta tagaacaaac tatattgccg aacctcacgg tgctgttggt tatcttggat
     8401 tggaaaaaga aatggagaaa aaaccagata acattggcgt tttcttggaa actgctcatc
     8461 caattaagtt tttggatata gttgagccat tattaaataa taaattaccc attccgaaac
     8521 aaattgaaag tgttttgaat aaagaaaaag tgagaacaaa gattgctact tatgaagatt
     8581 taaaagaatt tctacttcgt aattaatgat ttaaaaaatt gaaaaacata atcatggatg
     8641 ccagcaataa agcaatcccg ataagttttt caagcaatgt agaggatatt gaagtactaa
     8701 ttttgtgacc cacaaaagct ccaatggtaa aaccaatcat cactggccaa gttttttcaa
     8761 tttgtatgtt tccttgtaaa taatgactta ttgaacctgt aacgctagtg attaatatca
     8821 taaaaagtgc cgttgcagta gctatttttg atggaatttt aaatactttc aacattattg
     8881 gtgttttcat aaaaccgcca cccattccaa aaagcccagc tatagtacca gataacatcc
     8941 caaagaataa aaccatttgc aaactaactc tgtaagcaac atgttttttt ctatttttaa
     9001 ttatatacct tggtttcaaa tgattcagtt tgtcaaagaa acccaatttt atattatctt
     9061 gatttctttt aataaaaaaa gaacaaccaa ttaacaaaac cattacagaa aaagctatct
     9121 caagattttt tggagcaatg taacttaaca ataaactacc aagaattact ccaatcattg
     9181 ttggcacttc taataaaatg cccattttaa aatcaacatt actttctttt ctatttaaaa
     9241 aagtggacat catagccgat ggaataagtg aaaacattgt agatccaaca gccaattcta
     9301 ttggataacc aaaaaaagct attaatatag gaacaataaa tattccgccg ccaaacccta
     9361 caatggaacc aaataggctg attaaaaaac ttatacaaat taataagaca atcaattgaa
     9421 agaaatttta ggagaattat ttatacagaa aaaatgtttt tccaaagtta ctaacttact
     9481 aaagaattaa tctcaattga aacttttttt cttcttaaat tatttgggtt ggcaaaattc
     9541 tgtaactatt cctaataatc ttataagaaa ggaaacctta aataattgca attttacaaa
     9601 aaataaaacc atgaaaaatt ctgtaaaaat aattgcctta attgttagtg ttattttcct
     9661 ttcttgtaat gaaaaaaagg aatctaaaac gcataaaaaa cctatagtag aaagaattca
     9721 accaaaagaa atttccttta catttcaaaa aagtaaagaa tggttgaatg gcaatactga
     9781 taccaaaaaa gcaatgaata tagttttggc tataaatcgt gttgataaaa gcaacctcat
     9841 caaattagat agcatcatag ttccaacaga tatatcgggc gatattgcct attatatgcc
     9901 atttccattg gatgtttcct ctttagaaga agtaaaaaaa ataatcgtat tttcttatgc
     9961 tacacaagca tttgccactt atgaatatgg cgttttaaca catgttggcc caacaagcat
    10021 gggaaaagaa aaagacaaaa cgccaactgg gttattcttt actaattgga aagctgaaga
    10081 aactaccagt acttttaatg atgaatggaa attgaaatgg aatttcaata ttgaaaacaa
    10141 actaggtgtt ggttttcatc aatatgaatt gccaggttat cctgcttcgc attcttgtct
    10201 tcgattgtta gaaaaagatg ctaaaatgtt atatactttt gccgatgaat gggttttgga
    10261 aagtgaccaa aaaattgagc taaaaggaac acccgtaata gtttttggta gctatgactt
    10321 tgaaggtcaa aagccttggt acgacttaat agaaaatcct aaagccttat caatttctaa
    10381 agagcaactt gaaaaagtta tttcgcctta tttaagtact atactaaaag aacaagcaaa
    10441 acgggaaaat aaataattta taaagccgta atttacgctt ccatttcgaa gaaaagaggt
    10501 tcaatcatca ctttgtcagc tagtgtttca actctttcta ttatttcgga gcaaaagaaa
    10561 aggcgttgtg ttttttcgat actcatactc aatcgcaaca ctattgcatc cacacgattt
    10621 ttgaataaaa tatcggcgtc atcaacaaca aacatacgaa tggtagaagc attaaatcct
    10681 gccgatgaaa acaaagcatt gatgcgattt ggtgttccaa tgagaatgtc taaaccaagt
    10741 gaaatgatgt ttttatcgta atcgatatcg cctttgtcgt gcacaccaag aatactcaaa
    10801 tcatggtaat ttgcatattt cagaaacaat tcttccattt ccaaaacctt ttctttactt
    10861 tctaccataa tcaaagcacg agtactttcg ccaactgttc gctccaagcg ttgaataacg
    10921 tttaaaacaa ttgtagttgt ttttcctgtt ccttttggcg attgaatcac agcatcagca
    10981 ccacttttga tggttgaaaa tgtttctatt tgcatttcgt ttgcttgggt cagtccgttt
    11041 tcaacaagtg ctttttggag gttagggttt atttttttta aattcatatt tgggctacaa
    11101 gtagcgattt ttattatgca aaaatttctt tattttatta tcgttgtgat atattcttaa
    11161 tacttgacaa tcaggtctta ctgctattta cttgcaaaca atttcacatc cgtttctgag
    11221 atttctgttc ctcctaaaat gattaatcgt tctaccacat ttcgcaattc acggatattt
    11281 cctgtccaat catattcttg aagtaatttg atagcatctt ttgaaaatga ttttgaaaca
    11341 cttccttgtt cttgagcaat tttattcgaa aaatattcaa ttaataacgg aatatcatct
    11401 cgtctatcgt tcaaagctgg aactttgatt aaaatgaccg ccaatcgatg gtataaatct
    11461 tcacgaaaac gtccttcttc aatttctttt ttcaagtctt tattagtagc agcaactaca
    11521 cgaacatcta ctttaatgtc tttgtctgcg cctactcgtt gcaccaaact ttcttgcaaa
    11581 gcacgcaaca ctttggcttg tgctgccaaa ctcatatcgc caatttcatc taagaaaatt
    11641 gtaccgccat tagctgcttc aaattttcct gctctatctt tcacggcaga tgtaaacgca
    11701 ccttttacat gcccaaaaag ctcactttca attaattcag atggaatagc agcacagttt
    11761 acttcgatta aaggaaattc tgatcgtgca cttttgtcat gcaactgatg cgccaccaat
    11821 tcttttcctg ttccgtttgg tccagtgatt aaaactcttg catcggttgg tgctactttt
    11881 tcaatcattt ctttaatctg attgattggt gctgagttcc caaccatttc ataattttta
    11941 gaaacttttt tctttagaat tttattttca acaactaatt gttttttatc caatgcatta
    12001 cgaaccgtat ttaacaacct attcaaatca ggtggttttg aaatataatc aaaggcaccc
    12061 aatcgcatcg tattaacagc agtttccaaa tcgccatgtc cagaaatcat gaccattgga
    12121 atttcaggct ttattttttt aacagcttct aacagttctt cgccgttcat ttttggcatt
    12181 tttatatcac aaagcaccaa atcataatcg tcatttttga ttttatcaaa acctattttt
    12241 ccatcttcag cttcatccac ttggtatgtg gtactttctt cggataatat tttcaccaat
    12301 acacgacgga tagcggcttc gtcttcaata attaagattt ttgacatagt ttaaaaacag
    12361 attttaaatt aaaattttta actgtacaaa agtactaatt taactttagt atctcgatgt
    12421 ttgtagtaag acgaatagat tttatacttt tacaactcaa aattatgata tatgtcaact
    12481 gcaaagaaag attataaaag aattaccacc aagtcattat ttgacatgaa aaaaaatggt
    12541 gaaaaaatat ccatgctaac cgcttatgat tataccatgg caaaaattgt tgattcggct
    12601 ggcgttgatg taatattagt aggcgattca gctagtaatg ttatggcagg gcatgaaact
    12661 acgctaccaa tcactttaga ccaaatgatt tatcatgctt catctgttgt tcgtgctatc
    12721 gaacgttctt tagttgtggt tgatttaccg tttggaagtt atcaatccga tccaaaagaa
    12781 gctttacgtt ctgctattag aatcatgaaa gaaagtggcg gacatgctgt gaaattagaa
    12841 ggcggaagtg agattaaaga aagcatcaaa cgtattttaa atgccggaat tcctgtgatg
    12901 ggtcatttag gattaacacc acaatcaata tacaagtttg gaacttacac ggttcgtgcc
    12961 aaagaagaag aagaagccga aaaattaatt gaagatgcta aaatgctaga gcgaattgga
    13021 tgttttgcct tggttttaga aaaaattcca gcttcattag caactaaagt agcacaaagc
    13081 atttctattc cagttattgg aattggagca ggtggcggcg ttgacggaca agtacttgtt
    13141 attcatgaca tgcttggaat gaataacgaa tttaacccaa gattcttaag acgttatttg
    13201 gatttgtacg aacaaatgaa taaagctatc ggtcaatatg ttaccgatgt aaaatcgaaa
    13261 gattttccta atgaaagtga gcagtattaa cgagattgaa ggttgtagat tgtaaaacat
    13321 aataattgcg atgactgata aaatattcac tacaaaagat aaccttcaaa ttctgcacga
    13381 agacaaccat attatagtgg ttaacaaacg tgttggcgat attgttcaag gtgataaaac
    13441 gggtgacaaa ccattatctg aagtagtaaa agaatatatt aaagacaaat acaataaacc
    13501 tggcgaagta tttcttggag ttgtacatcg tttagacaga ccaacttcag gtattgtagt
    13561 ttttgctaaa accagcaaag cattaacgcg tttgaatgaa ctattcaata aacgagaaac
    13621 ccaaaaaact tattgggcaa tcgtaaaaaa ttatccgcca aaagaaaacg atattttggt
    13681 gcatttttta aaacgaaatc caaaaaacaa tacttccaaa gcgcacatca aagaagttcc
    13741 tgaaagtaaa aaagcaagtt tagaatatac cattattaaa aagcttgaca gctattatgc
    13801 gcttgaaatc aatcttcata ctggaagaca tcatcaaatt cgagcacaat tacaagccat
    13861 tggttgtcca ataaaaggtg atttaaaata cggttttgac cgcagtaatc ctgatggagg
    13921 tattcacctt cacgctagac aattagtatt tattcatcca gttgcaaaaa tagaagtaag
    13981 tcttattgct cctactccaa atgatgtaat ttggaatgca attggttaac aaactctatt
    14041 ctattattat taatattaca aatttctttt taatataaat ttatatttgt attattgact
    14101 aagcaaatgt atccattatg aaaaaatata tctattcatt cttcttgatt tcaagtacaa
    14161 tggttttttc tcaagaccat tattcaggaa ttagcacatc aaatcgtgtt gggattttaa
    14221 atgttaccat taacccagct gaattgccaa atctttccaa aaaatttgaa gtaaatattt
    14281 atggaatgag ttttaatgtt gctaacaaca aaattggatt tagtgatttt aattcggaca
    14341 aagattttga agaacttctt tttcaaggaa caagtcctgt aaatgctcgt attgacactg
    14401 acatcatcgg tcctggtttt gcaatgcaat tgggaaaatg gggttttgct tttaccacaa
    14461 aagcctatgc caaatttgac atggtagatg ttgatccaac gattggaaac gcaataacaa
    14521 ataataattt agttcttaat acaactcttt taaacaatcc aaataatcaa agattaagtg
    14581 gaacttctta tggtgaaata ggtttatcag caggtagaac gttttttgaa aatgacaaac
    14641 acaaatttag tgctggggta actttaaaaa tacttttccc aggttcgtat tccaattttg
    14701 gtttgagtca attagatgga acaattaccc aaagtttggg caattcttat ttaaccacaa
    14761 atagtcctgc tagtttgaat attgcttatt cgggaaattt agcaaacagt ttctctaatt
    14821 ttgatgatta tagcaaatcc atttttggcg gtttaaatgg tcttgctgga gatattggct
    14881 tcaactatca attaaaaagc gatacaaaat ataaattaaa tgctggttta tccattagaa
    14941 atattggaag tatgactttt aaagatgaga ataattacaa tactagttat actttaaata
    15001 ttccaagtag caatccttta gatttaagtt tgttcgacaa tattgacaac cttagtgagg
    15061 tggaagacat tcttacaaac aatggatatt taactactca acctagaaat aaaacagatt
    15121 ttaatgttaa gctacctaca acggttaata cttatgtaga tgtaaaagta gtttctaaat
    15181 tatatgtaac tgctttttta caacaaaagc taaataaaga ccgagacaac gagcaggtga
    15241 caacaccaaa catttttagt attacaccaa gagtaaattt aggttttttt gaaggctaca
    15301 ttcctttaac ttttcatgaa atttcgggca ctaatgttgg tttaggcttt agattaggcg
    15361 gtttttattt aggaacaagt tctatggtta ccgcgttaat tagtgatagc aaacaaggtg
    15421 atttatattt tggtttccgt tttggttttc tttaaaacca ttcacagttt taatttcaat
    15481 taaaaattgt aacttgtcat aaactttaat gttggtttat gctatttaaa acttcaatca
    15541 cctatagaaa aaaaacactg cttaagctat taattgcaat cgaaaagcat gaagaagaaa
    15601 ttaatcaagc attgtataat gattttaaga aacctgaatt tgaaagttat ttaaccgaaa
    15661 cttattttgt cctttcagaa ttaaaactga ctatcaagaa tatcaataaa tgggcaaaac
    15721 caaaa
//
LOCUS       DAOOBH010000045        15357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5014_length_15357_cov_7.217945, whole genome shotgun sequence.
ACCESSION   DAOOBH010000045 DAOOBH010000000
VERSION     DAOOBH010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15357
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5014_length_15357_cov_7.217945"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /gene="clpX"
                     /locus_tag="PK218_06250"
     CDS             <1..773
                     /gene="clpX"
                     /locus_tag="PK218_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPW98142.1"
                     /translation="SILTRLLQAADYDVAKAERGIVFIDEIDKIARKSDNPSITRDVS
                     GEGVQQALLKLLEGTVVNVPPKGGRKHPDQKFVEVNTQNILFIAGGAFDGIDRIISKR
                     LNRQAVGYSTSRNVDAIDKDNLLQYVIPKDIKDFGLIPEIIGRLPVLTHMDPLDAETL
                     RAILTEPKNALIKQYKKLFDMDNVKFTIDNDALDYIVQKALEYKLGARGLRSLCEAIL
                     TDAMFELPSSEDKELHINREFTEDTLGKNLLKRLEIAS"
     gene            complement(816..3035)
                     /locus_tag="PK218_06255"
     CDS             complement(816..3035)
                     /locus_tag="PK218_06255"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW98143.1"
                     /translation="MKSLFTAFLVLSSTAFFAQETEKNQQNTTVNDTIKSKKITELEG
                     VTIFGNKKQFIKVDSDKTTVSIKENPMLSSGTALEAVKKLPGVITSPTGSLSLNGKAV
                     TVYIDNAPSTLSGQDLQNYLSSLPAKAIEKVELIYNPGAAFDANASGSIVNIITSSRR
                     MKGINASFNINYNFNKYQKPSPQILLNGKEKDLSWQTMLGYNYIDSETRITTNQDFTS
                     FSPVKTLRQENFSVNTQRNLYWRTGTNYRISEKSNFLLNYNMNLGNDRNVFEATTNGE
                     NIDYQNSGVGKTKNQNHELSLQYKTKLDTLGRTLDVTGFGNLFHQNPISSSNAKDVIS
                     NESYFNIGTSDFDLKNYYLKYDFNIPFEKYKFSIATGGKYNILKVNNVGEYFINTPTV
                     NRINFDYSENNLAFYAEAKKRVNKFSFTLGLRFEDYNVERISSTVVDKIKFRNTNLFP
                     NISAIYEVNDNVNLSASYSKKINQPNYNTLDPNNSSTFDQYNSSQGNIFLNPTFFDNY
                     EFKISAMQFIQLGANFTRTKDNNLFIFSADADAAEPISNQTFQQFERFDTFSAYFNFP
                     IPLDYFFKGKEEFQKRMNYMDKMNYIYFNINYVKNDIRGFNFPYSNKAIWTYSLQSQI
                     ILPWDIKNTITYFIVDRGNWEIYKITKPIQQFDISFNKEFMNKKLNVGLHCFDVFNVN
                     QIDALIGGQNLNSTFRKKEDSRTFRISLTYNFGNLKLQKENTDIDIEKVQSGGGFVK"
     gene            complement(3090..3986)
                     /locus_tag="PK218_06260"
     CDS             complement(3090..3986)
                     /locus_tag="PK218_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPW98144.1"
                     /translation="MRTKLYLAFFCVAFFWGTTFLGIKIGVETIPPFILAGIRNFISG
                     FIIITYLFYQKKLEKISIHQFARAFLLSMLMIVLANGLTTYAEKYISSGLASLISTLN
                     PLFVLLINLFLGYEKLSFKTILGIFFGMLGMFLLYQSRIEELLVPEYRFGILAIFIAI
                     ISWSIGTIITKKGSTKTLSMLMNVSLQMIIAGVVLTSIQFTLTPNITYETWSTRSILA
                     MIYLALFGSVVGYVAFSYLLSQISSTKVTVLSYANVVVALFLGWLLLDEIITLRIILA
                     TLFIISGVFIVNYKRKKLRSDL"
     gene            complement(3993..4133)
                     /locus_tag="PK218_06265"
     CDS             complement(3993..4133)
                     /locus_tag="PK218_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98145.1"
                     /translation="MKKILAILILLGFLTSCGPHRMKCGPGRRCMVEMPKDKLVLDKN
                     VV"
     gene            complement(4130..5665)
                     /locus_tag="PK218_06270"
     CDS             complement(4130..5665)
                     /locus_tag="PK218_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPW98146.1"
                     /translation="MPFPIINSIASWVLKQRIHQIELFLKYPHEVQNELLMNLIRNCE
                     ETVVGKKYDFESIKNYTTFAERVPVATYEELEPMIELTRKGAQNVFWHKPIKWFAKSS
                     GTTNAKSKFIPVSETALMDCHYKGSKDLLCMYLNNNENSELFLGKSLRLGGSSQIYEN
                     NNTYFGDLSAILIENMPMWAEFSSTPSNKISLMSEWETKLSAIINETKVENVTSFAGV
                     PSWMLVLLNRILTETGKDNLFELWPNLEVYFHGGVSFEPYREQYKKILPSTNFKYYEI
                     YNASEGFFAIQDLNDSNDLLLMLDYGIFYEFIPMDTFGTENQKIIRLSDVELYKNYAI
                     VITTNSGLWRYMIGDTVRFTSLSPYRIRVSGRTKHYINVFGEELMIENTDRAIAKTCS
                     ELNCDVKDYTVAPIFMKNKEKGSHEWMIEFKKYPDDIKLFQKLLDENLQTINSDYEAK
                     RYNNMTLNNLKVNVARENLFYDWLKENNKLGGQHKIPRLSNERDYLEQLKNMMVEVQN
                     KTQ"
     gene            complement(5710..6324)
                     /locus_tag="PK218_06275"
     CDS             complement(5710..6324)
                     /locus_tag="PK218_06275"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPW98147.1"
                     /translation="MKSLLKIIIIAVALLSLSFVSNENKDFVVVIDAGHGGKDFGAKH
                     TTFLEKELVSAISQKIAKINTDKNVKIYFTRTSDEFIELQKRVEMINEIKPNLLLSLH
                     VNQNKNTETNGFEAYVSDKNSNFEKSEELAKKLVTLFENQTPLKNRGVKKGMLYVLKK
                     TEAPAIAIEIGFITNEKDRSYVCNEQGQTQIAQSILDFVNTIKN"
     gene            6411..7205
                     /locus_tag="PK218_06280"
     CDS             6411..7205
                     /locus_tag="PK218_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HPW98148.1"
                     /translation="MTYEGVLTKMQTEFQAPIQYYLVFENSFLNLNQLLDKSIEINFT
                     GYQCLNCGKKKKIFRQGFCYDCFMSSASAGDWIMKPELSTAHLDIEDRDLEYEKKVQL
                     QPHIVYLALSSEVKVGVTRKTQVPTRWIDQGAVQAIPIVEVPNRYLAGITEVALKDHY
                     SDKTNWQKMLKNEVPAADLIKEKASIQYLIPKEVQEYFHLEKDDLYQLQFPVLHYPKK
                     VISLNLDKTPIFSGKLMGIKGQYLLFEDGTVFNVRTFEGYVVKISV"
     gene            complement(7253..>8274)
                     /locus_tag="PK218_06285"
     CDS             complement(7253..>8274)
                     /locus_tag="PK218_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPW98149.1"
                     /translation="SEAVKIPAFLNCTLTAKANTVLYDNLTLKDVAGKLIIKDQKVTL
                     ENGKTNIFGGQIGFNGAVSTKEKTPKFNMNLGLNNVDIQQTFTQLDMLKKIAPIAGII
                     NGKLNSTIKVSGNLDAKEMTPDLKSLSGDLIGQLLSTTINSSNSTLISALDSKLSFID
                     LNKLNLNDLKAALTFKDGKVNIKPFDLKYQDISVNIGGSHGFDQSMNYDLKFNVPAKY
                     LGTEVNNLIAKLTPADANKVETIPINAMMTGNFSNPKVTTDLKQATTNLVTNLVKQQK
                     DKLVNTGKDALNNIINNATKPTTDSTKTDVKTDVKNTATGILKGLLGGKKKEEPKKEE
                     PKKTE"
     assembly_gap    8275..8374
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8375..10020)
                     /locus_tag="PK218_06290"
     CDS             complement(<8375..10020)
                     /locus_tag="PK218_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HPW98150.1"
                     /translation="MLKKILKIVGIVLILLVVSLFAIPYFFKDQIKAKIEKTINEKVD
                     AKVAFADADLSLFRNFPNASVRIDKLSIINKAPFEGDTLVAFEELNLKMSIKELFKGE
                     DEPINIDGISSKNGLINIIFNKDGIGNYDIALKDDKDQKDDDESKPMAFKIKEYSVEN
                     FRFQYYDEASKMKMVLDSINHEGKGDFAASKLDLDTKTTAKVSLDMDKANYLKNVALS
                     LDAVLGIDMEQSKYTFKENKAKINELPLEFNGFIQMVEAGQVYDLTFKTPTSSFKNFL
                     GLIPAQYASNIKDVKTEGDFTVNGFAKGTYTDTTIPKFNIEIASNNASFQYPNLPKSV
                     QNIMIDTRIINETGVINDTYVNLDKLSFRIDQDVFNAKANIRNIAENALVNADLKGTI
                     NLGNVTKAYPVKLDKPLSGILKADVKTEFDMQSVEKSDYARIKNSGNIDLSGFNYTDE
                     NGKAMTINKAVVEFNPSRVNLKQFNAVTGKSDLAVNGVLENFYGFVFKNQELKGDFNL
                     SSNQIAVDDFMTAETPKENEKKNSEAVKIPAFLNCTLTAKA"
     gene            10197..11330
                     /gene="folK"
                     /locus_tag="PK218_06295"
     CDS             10197..11330
                     /gene="folK"
                     /locus_tag="PK218_06295"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPW98151.1"
                     /translation="MKQHQVILSLGSNQGNRLENIERCIAQIQQEIGTVIRVSKLYET
                     PAWGFESEKFYNCALVMHSSKSANQILEEVLVLELKLGRIRSETEGYQSRIIDIDVIA
                     FDEEIIDNEKLKVPHPEMQNRLFVLVPMRDLNLDWRHPILQKYLHELVVLSEDRSNCR
                     LVKSLENPLTKIALNHFNYIAIEGNIGAGKTTLANKFAEDFNAKTVLERFADNPFLPK
                     FYEDQSRYAFPLEMSFLADRYQQTADDLAQFDLFKDFIVADYHIFKSLIFAKVTLAED
                     EFRLYKTMFDIIYKEMPKPDLYIYLYQNTERLLENIKKRGRDYEQEIPAEYLEKINSG
                     YLDYIKTQKDLNVLIIDVSDRDFIKSQEDYNFILNEIQTKISR"
     gene            complement(11327..11857)
                     /locus_tag="PK218_06300"
     CDS             complement(11327..11857)
                     /locus_tag="PK218_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPW98152.1"
                     /translation="MRKLENKELDRLTVTDFKEAKKTPIIIILDDIRSLHNIGSVFRT
                     SDAFLVEKIYLCGITAVPPNKEIHKTALGATETVAWEYEKDVLSLIERLKKENISIFA
                     IEQVENAIFLNDFEVEIEKKYALVFGNEVFGVNQKAIELCDGTIEIPQLGTKHSLNIS
                     VSTGIVIWDLFCKMKF"
     gene            complement(11936..12388)
                     /locus_tag="PK218_06305"
     CDS             complement(11936..12388)
                     /locus_tag="PK218_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPW98153.1"
                     /translation="MKKIILLTALALVGFNANAQTAKSAVKKTAAKIEAAKVDGPGMV
                     FENETIDYGTIQKGADGKREFVFINNGNKPVTISNAQGSCGCTVPTKPDGAIAPGARG
                     VIGVKYDTNRVGPFTKTVTITSDASETPKVLTIKGTIVDDKPATETKS"
     gene            12537..15143
                     /gene="mutS"
                     /locus_tag="PK218_06310"
     CDS             12537..15143
                     /gene="mutS"
                     /locus_tag="PK218_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPW98154.1"
                     /translation="MAAKEIKSKETPLMKQYNEIKRKYPDACLLFRVGDFYETFGEDA
                     VRASKILGIVLTKRGAGSETETALAGFPHHSLNTYLPKLVKAGLRVAICDQLEDPKMT
                     KTIVKRGVTELVTPGVAMNDEVLHSKSNNFLASIYFGKKLLGISFLDVSTGEFLVAQG
                     NEEYIDKLLQNFVPSEILIQKNNRNQFKEVFGEDFNTFYLEDWVFKEDYALETMMSHF
                     QTNSLKGFGIEELQDGIIASGAILYYLSETQHNKIKHITNIQRIAEDAYVWMDRFTIR
                     NLELYQSTAHNAVTLLDVIDKTLSPMGSRLLKRWLALPLKDCHKIRSRHEVVAYLKEN
                     QDVLKKIQHQIKQISDLERLISKVATGKISPREVIYLKESLDAIIPIKTLALESKQEA
                     VRIIGDNLHACDLLRDKIKTTLNQDAPVSINKGNAIAKGIHQELDELRAIAFSGKEFL
                     EAIEKRESERTGITSLKISFNNVFGYYIEVRNTHKDKVPTEWIRKQTLVNAERYITEE
                     LKEYESKILGAEDKIHQIETHLFEQLVNWIATYIKPVQLNANLVAQLDCLTSFAQLAI
                     DNNYVCPVLDDSFELEIKEGRHPVIEKQLPVGVPYISNDVFLDRETQQLIMITGPNMS
                     GKSAILRQTALIVLLAQMGSFVPAEAVRMGIVDKIFTRVGASDNISMGESTFMVEMNE
                     TASILNNISDRSLVLLDEIGRGTSTYDGISIAWAIAEFLHENPAQPKTLFATHYHELN
                     EMSELLPRIQNYNVSVKELKDTVLFIRKLVKGGSAHSFGIHVAKMAGMPQMVIQKAQK
                     LLKKLEKDHSSDVLNGIKSTKDEMQLSIFNLDDPLLEEIREDILNLDINTLTPVEALM
                     KLNELKRMLLKK"
BASE COUNT         5017 a         2456 c         2468 g         5316 t
ORIGIN      
        1 aaagtatttt aacacgttta ctccaagcag cggattatga tgttgctaaa gcagaaagag
       61 gtattgtttt tattgatgaa attgataaaa ttgctcgtaa aagcgataat ccatcaataa
      121 caagagatgt atctggcgaa ggtgttcagc aagcattatt gaaattattg gaaggaacgg
      181 ttgttaatgt tccgccaaaa ggtggaagaa aacatccaga tcaaaaattt gtggaagtaa
      241 atactcagaa tattttattt atcgctggtg gtgcttttga cggtattgac agaattattt
      301 ctaaacgcct taaccgtcag gctgttggat atagcacttc aagaaatgtt gatgcaatag
      361 ataaagataa tttattgcaa tatgtaatac caaaagacat aaaagatttt ggattaatcc
      421 ccgagattat tggtcgttta cctgttttaa ctcacatgga tccactagat gcggaaacct
      481 taagagctat tttaacagaa ccaaaaaatg ccttaatcaa acaatataaa aaattatttg
      541 atatggacaa tgtcaaattc accattgata atgatgcctt ggattatatt gttcaaaaag
      601 cgttagaata taaacttggt gcccgtggtt tacgttcatt atgcgaagct atcttaacgg
      661 atgctatgtt tgagcttcca agtagcgaag ataaagaact acatataaat agagagttta
      721 ccgaagacac tttgggtaaa aatcttttga agcgattaga gatagcctca taaatgaatt
      781 aaaaaaaatc cagcaattgc tggatttttt tgtttttatt tcacaaaacc gcctccgctt
      841 tgtacttttt ctatatcaat atcggtgttc tctttttgta gttttaaatt tccaaaatta
      901 taggtcaacg aaattcgaaa tgttctacta tcttcttttt ttcggaaagt agaattcaaa
      961 ttttgaccac caattaaagc atctatttga tttacattaa agacatcaaa acaatgcaac
     1021 ccaacattta attttttatt cataaattct ttgttgaatg aaatatcaaa ctgctgaatt
     1081 ggttttgtga ttttataaat ttcccaattt cctcggtcaa caataaaata ggtaattgta
     1141 tttttaatat cccacggtaa aatgatttgc gattgtaagg aataagtcca aatggctttg
     1201 ttggaatacg gaaaattaaa cccacgaata tcatttttaa cataattaat gttgaaataa
     1261 atgtaattca ttttatccat ataattcatt cgcttttgaa attcttcttt tcctttgaag
     1321 aaataatcta acggaattgg aaaattaaaa taagcactaa aagtatcaaa tcgctcaaat
     1381 tgttgaaagg tttggttgct aattggttct gcagcatcgg catcggcact aaaaatgaat
     1441 aagttattat cttttgttct agtgaaattg gcaccaagtt ggataaactg catcgccgaa
     1501 attttgaact cataattgtc aaaaaatgtt ggatttaaaa aaatatttcc ttgcgacgaa
     1561 ttgtattgat caaaggtgga actattattt gggtctaaag tattgtaatt cggttgatta
     1621 attttctttg aataagaagc tgataaatta acattatcat tcacttcgta aatggcacta
     1681 atatttggaa ataaattggt atttctaaat ttgattttat ctacaacggt actcgaaatt
     1741 cgctcaacat tataatcctc aaaacgaagt cctaaggtaa aactaaattt attaacacgc
     1801 tttttggctt cggcataaaa tgctaaatta ttctcactgt aatcaaaatt aattctattg
     1861 actgttggtg tgttgataaa atattcgcca acattattta cttttaaaat attgtatttc
     1921 cctccagttg caatcgaaaa cttatacttt tcaaatggaa tattgaaatc gtattttaaa
     1981 taataattct tcaaatcaaa atcggaagtt ccaatattaa agtaactttc attagaaatt
     2041 acatctttgg cattcgaact tgaaattgga ttttgatgaa acaaatttcc aaaaccagta
     2101 acatctaacg ttcttcctaa agtgtctaat tttgttttgt attgcaaact taattcgtga
     2161 ttttggtttt tagtttttcc aacacccgag ttttgataat caatgttttc tccatttgtt
     2221 gttgcttcaa aaacatttct atcattgcct aaattcatat tgtaatttaa taaaaaatta
     2281 cttttttcag aaattctata gtttgttcct gttctccagt ataaatttcg ttgtgtattt
     2341 acagagaagt tttcttggcg caatgttttt acgggcgaaa acgacgtaaa atcctgatta
     2401 gtagttatcc tagtttcact atcgatataa ttataaccca acatggtttg ccaactcaag
     2461 tctttttctt ttccgtttaa aagaatttgc ggacttggtt tttggtattt attgaaatta
     2521 taattgatgt taaaactagc attaattcct ttcattcgtc tagaacttgt aataatattg
     2581 acgatggaac cacttgcatt tgcatcaaaa gcagcacccg gattatagat taattccacc
     2641 ttttctattg cttttgctgg taatgacgaa agataatttt gtaaatcctg accactcaaa
     2701 gtgcttggag cattgtcaat atataccgtg acagctttgc catttaaaga caaactaccc
     2761 gttggcgaag taatgactcc aggcaatttt tttacagctt ctaaagcagt tcccgagctt
     2821 aacattggat tctctttgat gctaacggtt gttttgtctg aatcaacttt tataaattgt
     2881 tttttgttgc caaaaatggt aacaccttcc aattcggtaa tttttttgct tttaatagtg
     2941 tcgttgactg ttgtgttctg ttgatttttt tcagtttctt gagcaaaaaa tgctgtgctt
     3001 gataaaacaa ggaatgcagt aaataatgac ttcatttgta attgattttg attgatgata
     3061 atcaatatga cttttcaatt acctatttgt tacaaatcag aacgtaattt ttttctttta
     3121 taattcacga taaagacacc actgatgata aataaagtag ccaaaataat tcgtagtgta
     3181 attatttcat ccaataacaa ccagcctaaa aataacgcta ctacaacatt tgcataagat
     3241 aagacggtaa cttttgtaga agaaatctgc gatagtaaat aactaaaagc gacataaccc
     3301 acaaccgaac caaataatgc taagtaaatc atcgccaaaa tacttcttgt actccaagtt
     3361 tcataagtta tatttggcgt taatgtaaat tgaattgaag ttagtacgac accagcaata
     3421 atcatttgca aacttacatt catcaacata gataaggttt ttgtgcttcc ttttttggta
     3481 atgattgtcc cgatagacca acttataata gcgatgaaaa tagctaaaat tccaaaacgg
     3541 tattctggta caagcagttc ttcaattctg ctttgataca ataaaaacat acccaacatg
     3601 ccaaaaaata ttccgagaat agttttgaat gataattttt cataacccaa aaagagattg
     3661 atgagcaata caaacaatgg gtttagagta gatattaaag aagctaaacc actagaaatg
     3721 tatttctcag cataagttgt caatccgttg gcgagaacaa tcatcaacat cgaaagtaga
     3781 aaagctcttg caaattgatg aatggaaatc ttctctaatt tcttttgata aaaaaggtag
     3841 gttataataa taaatccgga aataaaattg cgaattcctg ctagaataaa tggtggaatg
     3901 gtttcgacgc caatttttat tcctagaaaa gtggttcccc aaaaaaaggc aacacaaaaa
     3961 aaagcgaggt ataattttgt tctcaaaaag tattaaacta catttttatc aagaacaagt
     4021 ttgtcttttg gcatttcaac catacatctt ctacctggac cacatttcat tcgatgaggc
     4081 ccacaagatg ttaaaaatcc taaaaggatt agtattgcaa gtattttttt cattgcgttt
     4141 tattttgcac ttcaaccatc atgtttttca attgttccaa ataatcccgt tcgttggaaa
     4201 gtcttggtat tttatgttgt ccacccaatt tgttgttttc ttttaaccaa tcgtaaaaaa
     4261 ggttttctcg tgctacattg actttcagat tatttaaagt catgttattg tagcgtttgg
     4321 cttcgtagtc tgaattgatg gtttgtaggt tttcgtctag taatttttga aatagtttaa
     4381 tgtcatcggg atatttttta aactcaatca tccattcgtg tgaacctttt tctttgttct
     4441 tcataaaaat tggcgcaacg gtataatctt ttacatcaca gtttaattcg gaacaagttt
     4501 tggcaattgc cctatcggtg ttttcaatca tcagctcttc gccaaaaaca ttaatataat
     4561 gttttgttct tcccgaaact ctaattcggt agggacttaa agatgtaaat cgaacggtgt
     4621 cgccaatcat atagcgccac aaacctgaat tagtggtgat gactatggca tagtttttat
     4681 acaattcaac atccgacaaa cgaataatct tttgattttc ggtgccaaaa gtatccattg
     4741 gaataaattc gtagaaaatt ccataatcca acatcagcaa taaatcatta ctatcattca
     4801 aatcttgaat ggcaaaaaat ccttcagaag cattgtaaat ttcgtaatat ttgaaatttg
     4861 tactcggtaa tattttttta tattgttcgc gatacggttc aaaactcacg ccgccatgaa
     4921 aatatacttc cagatttggc cacaattcaa agagattatc tttgccagtt tcggttaaaa
     4981 ttcggtttaa tagaaccaac atccacgatg gaacgccagc aaaactggtt acattttcta
     5041 ccttagtttc gttaattatt gcggataatt tggtttccca ttcgctcatt aacgatattt
     5101 tgttgcttgg tgtgctacta aactctgccc acattggcat attttcaatc aatatggctg
     5161 acaaatcacc aaaataggta ttgttatttt cataaatttg agagcttcca ccaagtcgca
     5221 aactcttacc caaaaacagc tcggagtttt cattattatt caaatacata cacaacaaat
     5281 ctttgctgcc tttgtaatga caatccatca aagccgtttc gctcactgga ataaatttac
     5341 tcttggcatt ggttgtacca ctcgattttg cgaaccattt gattggtttg tgccaaaata
     5401 cattttgcgc accttttctg gtgagttcaa tcatcggttc caactcttca taagtagcaa
     5461 ctggaactct ttcggcaaag gtggtgtaat tttttatgga ttcgaaatca tatttttttc
     5521 caacaacggt ttcctcacaa ttgcgaatta agttcataag caattcattc tgaacttcgt
     5581 gtggatattt tagaaacaac tcaatctgat gaatgcgctg tttcaaaacc caggaagcta
     5641 tagaattaat tattggaaat ggcatctatt ttggtgttag attataggtt tttggaataa
     5701 ggcaggaaat taattcttaa ttgtatttac aaaatctaaa atgctttgag caatttgggt
     5761 ttgaccttgt tcattacaaa cataacttct gtctttctca ttagtaataa atccaatttc
     5821 aattgcaatt gctggtgctt cagtcttttt caaaacataa agcatacctt ttttgacacc
     5881 acggtttttt aaaggtgttt ggttttcaaa tagggtaact aattttttcg ccagttcttc
     5941 ggacttttca aaattagaat ttttatcaga aacataggct tcaaaaccat ttgtttcggt
     6001 atttttattt tgattaacat gtaaagaaag cagtaagttt ggcttgattt cgttaatcat
     6061 ttctactctt ttttgcaatt caataaattc atccgaagta cgtgtaaaat aaattttaac
     6121 gtttttatca gtattgattt ttgcaatttt ttgtgaaatg gcacttacca attctttttc
     6181 aagaaaagta gtgtgttttg cgccaaaatc ttttccgcca tgaccagcat caattactac
     6241 aacaaagtct ttattttcat tgcttacaaa cgataaactc aataaagcaa cagcaataat
     6301 gatgattttt aatagacttt tcataataga tagttttggt tatacataat aacaacgcta
     6361 atatagcatt tttattatat ttttatacaa aatttttcaa atcgatatac atgacttacg
     6421 aaggagtttt aaccaaaatg caaaccgagt ttcaagcacc aattcaatat tatctagtgt
     6481 ttgaaaatag ttttttgaac ctaaatcaat tattagacaa atcgatagaa attaatttca
     6541 cagggtatca atgtttgaat tgtggcaaaa agaaaaaaat tttccgtcaa ggattttgtt
     6601 atgattgttt tatgtcatca gcttctgctg gcgattggat tatgaaacct gagctttcaa
     6661 cggcacatct cgacattgaa gatagagatt tagaatacga gaaaaaagtg caactgcaac
     6721 ctcacattgt gtatttggca ttgtctagtg aagtaaaagt tggtgtaacc agaaaaaccc
     6781 aagtgcctac tagatggatt gaccaaggcg cagtacaagc cataccaatt gtagaagttc
     6841 ctaatcgtta tttggctgga attactgagg ttgcactcaa agatcattat tccgataaaa
     6901 ccaattggca aaaaatgttg aaaaacgaag tgccagcggc cgatttaatc aaagaaaaag
     6961 catcgataca atatctaatt ccaaaagaag ttcaagaata ttttcatctt gaaaaagacg
     7021 atttgtacca attacaattt ccagtgttgc actatccaaa aaaagtaatc agtttaaatc
     7081 tagacaaaac accaattttt tcaggaaaac taatgggtat taaaggacag tatttacttt
     7141 ttgaagacgg cacggttttc aacgttcgta cgtttgaagg ttatgtggta aaaatttcgg
     7201 tttagtatta tccataaaca acttctttta gaatttctta aagggattgt tattattccg
     7261 ttttcttcgg ttcctccttt ttcggctctt ctttcttctt tccgcctaac aatcctttaa
     7321 gaatacccgt tgccgtgttt tttacatcgg ttttcacatc cgttttagtg ctatcagtag
     7381 ttggttttgt ggcattattt ataatgttgt ttaacgcatc ttttccagta ttaaccaact
     7441 tatctttttg ttgttttacc aaattggtta ccaagttagt ggtcgcttgt tttaaatcgg
     7501 tagtaacttt tggattcgaa aaatttcccg tcatcatggc gtttatcgga atggtttcta
     7561 ctttgtttgc atcggctggt gttaatttcg caatcaaatt attgacttcg gttcctaaat
     7621 atttggcagg aacgttaaat tttaaatcat aattcatcga ttggtcaaaa ccatgactac
     7681 caccaatgtt taccgaaatg tcttggtatt tcaggtcaaa aggtttgatg tttacttttc
     7741 cgtctttaaa agtcaacgcc gcttttaaat cgtttagatt taatttattc aaatcaatga
     7801 aactcaattt gctgtctaaa gccgaaatta atgttgaatt gctcgaatta atcgttgttg
     7861 agagcaattg cccaatcaaa tcacccgata agctctttaa atcgggtgtc atttctttag
     7921 catccaaatt cccgcttact ttgatagtcg aatttaattt accattaata attccggcaa
     7981 ttggtgcaat ttttttcaac atatctagtt gggtaaacgt ttgttgaata tcaacgttat
     8041 ttaatcccaa attcatattg aattttggtg ttttttcttt ggtagaaact gcaccattaa
     8101 acccaatttg acctccaaaa atattggttt ttccgttttc taaagtaact ttttggtctt
     8161 taattatcag tttccctgca acatctttca gcgtcaaatt atcatataaa accgtgtttg
     8221 cttttgcggt aagcgtacaa tttaagaacg ctgggatttt tacagcttca gaatnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngctttt gcggtaagcg tacaatttaa
     8401 gaacgctggg atttttacag cttcagaatt tttcttttcg ttttcttttg gagtttcagc
     8461 agtcataaaa tcatctaccg caatttgatt agacgacaaa ttgaaatcgc ctttcaactc
     8521 ttgattttta aatacaaatc cgtagaaatt ttctaaaaca ccgttaacag ccaaatcact
     8581 ttttccggta acggcgttaa attgtttcaa attcacacga ctaggattaa attctacaac
     8641 cgctttgtta atcgtcattg ctttaccgtt ttcgtcggta taattaaaac cagacaaatc
     8701 gatgttacct gagtttttaa ttcgtgcgta atcgcttttt tccaccgatt gcatgtcgaa
     8761 ttcagttttc acatcggctt tcaaaattcc cgaaagtggt ttgtctaact tcacaggata
     8821 ggctttagta acattgccaa gattgatagt tccttttaaa tcagcgttta ccaaggcgtt
     8881 ttcggcaatg tttctaatgt ttgctttagc attaaagaca tcttggtcaa ttcggaatga
     8941 taatttatcc agatttacat aagtgtcatt tattacacca gtttcattga taattcgggt
     9001 atcaatcatg atgttttgaa ccgattttgg caaattagga tattgaaacg aagcattgtt
     9061 agaagcaatt tcgatgttga attttggaat cgttgtatca gtatacgttc cttttgcaaa
     9121 accatttacg gtaaaatcgc cttcggtttt tacatcttta atattcgatg catattgtgc
     9181 aggaattaga cctaagaagt ttttaaaaga tgaagttggc gttttaaaag tcaaatcata
     9241 cacttgtcca gcttcaacca tttgaatgaa accattaaat tctaaaggta attcattgat
     9301 ttttgctttg ttttctttga aagtatattt gctttgctcc atgtcaatac caagaacggc
     9361 atctaaagac aaggcaacat tttttaaata atttgctttg tccatgtcca aagaaacttt
     9421 tgcagttgtt ttggtgtcca aatcgagttt agaagcggca aaatcacctt ttccttcatg
     9481 atttatagaa tctaaaacca ttttcatttt agaagcttcg tcataatact gaaatctgaa
     9541 attttcaacc gaatattctt tgattttaaa agccattggt ttgctttcat catcatcttt
     9601 ttggtctttg tcgtctttta aagcaatgtc ataattacca attccatctt tgttgaagat
     9661 gatgtttatc aaaccatttt ttgatgaaat tccatctata ttgattggtt catcttcgcc
     9721 tttaaataat tctttgatag acattttcag gtttaattct tcaaaagcga ctaaagtatc
     9781 gccttcaaac ggtgctttgt tgataataga tagtttgtca attcgcacac ttgcatttgg
     9841 aaaattccta aacaaactca aatcagcatc agcaaaagcc acttttgcat ctactttttc
     9901 gttgatggtt ttctcgattt ttgccttaat ttgatctttg aaaaagtaag gaatagcaaa
     9961 aagagatact actaaaagga tcaaaacaat tccaacgatt tttaaaattt tctttaacat
    10021 gatttaagtt tataattcaa cattgcgatt gacaaagata atgccatcaa atgtatgata
    10081 cattatagaa ttatgactaa atgtttgcaa aactttaata tttgaatgaa tagttatcaa
    10141 cacatcgggt ttttgaatag tattcttgaa taacttttat ttattttgca cttcctatga
    10201 agcaacatca agtcatttta tcgttaggca gtaatcaagg caaccgactt gaaaatattg
    10261 agcgttgtat tgcccagatt caacaagaaa ttggaacggt aattcgagtt tctaaactat
    10321 atgaaacacc agcttggggt tttgaaagtg agaaatttta caattgtgct ttggtgatgc
    10381 attcatcaaa atcggccaat cagattttgg aagaagtttt agtattagaa ctcaaattag
    10441 gaagaatccg gtcggaaaca gaaggttatc aatcaagaat tatcgatatt gatgtgattg
    10501 cttttgatga agaaattatt gacaacgaga agttgaaagt gccacatccc gaaatgcaaa
    10561 atcgtttgtt tgtattggtt ccaatgcgcg atttaaattt ggattggcgt catcctattt
    10621 tacagaaata tttacacgaa ttagtagtgc tttcggaaga tagaagtaat tgccgattag
    10681 tgaaaagttt ggaaaatcca ttgactaaaa ttgctctaaa tcactttaat tatattgcga
    10741 ttgaaggaaa tattggtgct ggaaaaacca cattggctaa caaatttgca gaagatttta
    10801 atgccaaaac tgttttagaa cgatttgctg acaatccatt tttaccaaaa ttttatgaag
    10861 atcaaagtcg ctatgctttt ccattagaaa tgtctttttt agcagacaga taccaacaaa
    10921 cagccgatga tttggcacaa tttgatttgt ttaaagattt tattgttgcc gattatcaca
    10981 tttttaaatc gttaattttt gctaaagtta ctttggcaga agatgaattt agattgtaca
    11041 aaacgatgtt tgacatcatc tacaaagaaa tgccaaaacc tgatttgtat atttatttgt
    11101 atcaaaatac agaacgcctt ttggaaaaca tcaaaaaacg cggtagagat tatgaacaag
    11161 aaattccggc ggaatattta gaaaaaatca atagcggtta cttagattat atcaaaacac
    11221 aaaaagattt gaatgtgttg attattgatg tttctgatag agatttcatc aaaagtcaag
    11281 aagattataa ttttattttg aacgaaattc agactaaaat ttcccgctaa aatttcattt
    11341 tacaaaacaa atcccaaatc acaattccgg tacttaccga aatatttaag gaatgtttag
    11401 tgcctaattg tggaatttca atcgttccgt cgcaaagttc tatcgctttt tgattaaccc
    11461 caaacacttc gtttccaaaa actaaagcat attttttttc tatttctact tcaaaatcgt
    11521 tcaaaaaaat agcattttct acttgttcaa tggcgaaaat ggaaatattt tcttttttta
    11581 atctctctat caaagataaa acatcctttt catattccca agcaactgtt tctgtggcac
    11641 caagtgccgt tttgtgaatt tctttgtttg gcggaacggc ggtaattccg cataaatata
    11701 ttttttcaac caaaaaagca tcagaagttc taaaaaccga accaatatta tgaagactcc
    11761 taatgtcatc aagaataatg atgataggcg tttttttagc ttctttgaaa tcagtaacag
    11821 tcaatcggtc aagttctttg ttttcgagct ttctcatagt taaaattttg gcaaagatat
    11881 tcaaaaaaaa agctcccgaa aaatcggaag ctttcttaaa aaactatttt aataattaac
    11941 ttttagtttc tgtagctggt ttatcgtcaa cgatagttcc ttttattgtt aatacttttg
    12001 gcgtttctga tgcgtcagaa gtaatagtta ctgtttttgt aaatgggcca actctattag
    12061 tgtcatattt cacaccaatt actcctcttg ctccaggagc aattgcaccg tcaggttttg
    12121 taggcactgt acatccgcaa gaaccttgag cgttagaaat agtaactggt ttgtttccgt
    12181 tgtttataaa aacaaattct ctttttccat cagcaccttt ttggatagtt ccataatcaa
    12241 tagtttcgtt ttcaaaaacc attcctggtc catcaacttt agccgcttca attttagcag
    12301 cagttttttt aacagcagat tttgctgttt gagcatttgc attaaatcca actaatgcta
    12361 aagcagtaag taaaattatt tttttcattt ttaatgtttt ttaatgatta cggtacaaat
    12421 ctatttaatt tcatattaca acttaaaatt ttatacttaa gattaaatta atattataaa
    12481 tcagtttaac tttaaaataa ttaaattcgc tgtcaaatca ttttaaacca aaaacattgg
    12541 ctgcaaaaga aattaaaagc aaggaaactc cattaatgaa gcaatacaac gagataaaaa
    12601 gaaaatatcc cgatgcttgt ttgcttttta gagttggtga tttttatgaa acttttggag
    12661 aagatgccgt tcgtgcttca aaaattttag gaattgtatt gactaaacgt ggagcaggct
    12721 ctgaaacaga aactgcctta gctggatttc cgcatcattc tctaaatacc tatttaccaa
    12781 agttagtaaa agcgggactt cgagttgcca tttgtgacca gttggaagac ccaaaaatga
    12841 ctaagactat tgtaaaacgt ggtgttacgg aattagtgac accaggtgtt gccatgaatg
    12901 atgaggtttt acactccaaa tcaaataatt ttttggcgtc catttatttt ggaaaaaagc
    12961 tacttggtat ttccttttta gatgtttcta ctggagaatt tctagttgct caaggcaatg
    13021 aagaatatat tgacaaactg ttacaaaact ttgttccaag tgaaatttta attcaaaaga
    13081 acaatagaaa tcaatttaaa gaagtttttg gtgaagattt taatactttt tatttagaag
    13141 attgggtttt caaagaagat tatgctttag aaacaatgat gagtcatttt caaaccaatt
    13201 ctctaaaagg atttggtatt gaagaactgc aagatggaat tatcgcttcg ggagctatcc
    13261 tttattattt atcggaaacg caacacaata aaattaaaca catcaccaat attcaacgta
    13321 ttgctgaaga tgcttatgtt tggatggatc gatttacgat tcgaaattta gaattgtacc
    13381 aaagtacggc gcataatgca gttactttat tggacgtaat tgacaaaaca ttgtcaccaa
    13441 tgggttcgcg attgcttaaa cgatggttgg ctttaccgtt gaaagactgt cataaaataa
    13501 gaagtcgcca tgaagtagtt gcttatttaa aagaaaacca agacgttctc aagaaaatac
    13561 aacatcaaat caagcaaatt tctgatttag aacgtttgat ttcaaaagta gcaacaggga
    13621 aaatctcgcc acgtgaagtt atttatctaa aagaatcact tgatgctatt attccaataa
    13681 aaactttagc actggaaagt aagcaggaag ccgttcgaat tattggtgat aatcttcatg
    13741 cttgtgattt attaagagat aaaattaaaa ctactttaaa tcaagatgca ccggtttcta
    13801 tcaataaagg aaatgcaatt gcaaaaggaa ttcatcaaga attagatgaa ttaagagcca
    13861 ttgctttttc tggtaaagaa tttttggaag ccatcgaaaa aagagaatcg gaacgaacag
    13921 gaattacatc cttaaaaatt tcatttaata atgtctttgg ttattatata gaagttcgaa
    13981 acacacataa agataaagtg ccaacggaat ggattagaaa acaaacttta gtcaatgccg
    14041 aacgatatat aaccgaagag ttaaaagaat atgaaagtaa aattcttggt gctgaagata
    14101 aaatccatca aattgaaacc cacctttttg aacaattagt aaattggatt gcgacttata
    14161 taaaacctgt acagcttaat gctaatctgg tagcacaatt ggattgttta acctcttttg
    14221 cgcaactagc aattgacaac aactacgtat gccccgtttt agatgattca tttgaattgg
    14281 aaataaaaga aggtcgtcat ccagttatag aaaaacaatt gccagttggt gtgccatata
    14341 tttcaaatga tgttttttta gatagagaaa cccaacaact catcatgatt actggcccaa
    14401 acatgtcggg taagtcggca attcttcgtc aaaccgcttt aattgttttg ttagctcaaa
    14461 tgggaagttt tgttccagcc gaagcagtta gaatgggaat tgtagataaa atatttacca
    14521 gagttggtgc tagcgataac atttcgatgg gcgaatctac ttttatggta gaaatgaatg
    14581 aaacagcttc cattttgaac aatatttcag atagaagttt agttttgtta gacgaaattg
    14641 gtcgtggcac aagtacttat gatggaattt caatagcttg ggcaattgct gaatttcttc
    14701 atgaaaatcc ggcacagccc aaaactctat ttgctacaca ttatcatgaa ttaaatgaaa
    14761 tgtcggaact cttgcctaga attcagaatt ataatgtttc ggtaaaagaa ttaaaagaca
    14821 ccgttttgtt tattcgtaaa ttagtaaaag gtggtagtgc tcatagtttt ggaattcacg
    14881 tagcaaaaat ggctggaatg cctcaaatgg tgattcaaaa agcacagaaa ctattaaaaa
    14941 agctcgaaaa agaccattca agtgatgttt taaatggaat aaaatctaca aaagatgaaa
    15001 tgcagttaag tatttttaat cttgatgacc cacttttaga agaaatcaga gaggatattt
    15061 taaatttaga tattaatact ctaacacctg tggaagcttt aatgaaatta aatgaattaa
    15121 aaagaatgct tttgaaaaaa tagttttgat taattgattt tgtaaatcaa aggtttaaag
    15181 aatagtggtg tattttttat aaaaaaggtt tgtataattg aatttatgtt ttacatttgc
    15241 atccgcatta cagaacaagt agtgtcgttc ttataaatat ttaattgcga aaatagctca
    15301 gctggtagag cgcgaccttg ccaaggtcga ggtcgcgggt tcgagccccg tttttcg
//
LOCUS       DAOOBH010000046        15324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5035_length_15324_cov_5.698409, whole genome shotgun sequence.
ACCESSION   DAOOBH010000046 DAOOBH010000000
VERSION     DAOOBH010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15324
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5035_length_15324_cov_5.698409"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PK218_06315"
     CDS             complement(<1..337)
                     /locus_tag="PK218_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPW98155.1"
                     /translation="MQFIKKITSKELFKVTSLNSISVLIKIGVGFVSSKIIAVFIGPS
                     GMAIVGNFRNFITSIESISTLGLQNGIVKYVAEFEEEQLRLKKTISTIVFCLLIATFL
                     VSIILLLFSD"
     gene            446..2263
                     /locus_tag="PK218_06320"
     CDS             446..2263
                     /locus_tag="PK218_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPW98156.1"
                     /translation="MKRKRILSLFIVLLGIFLKGNAQDIELYQQFNGRYDFTFIGNTM
                     NTEANGFFTPCYALTSSSATLNLNVNDQIEKVFLYWAGSGTGDFDIQLNDVDISSQIN
                     FTAIQNSSLNHFFSAFADVTQQVLTTGNGIYTVSELDINPFIDSDTYCNNATNFAGWA
                     MLIVYKNTTLPINQINVYHGLQALSAAVVNVQTQITITLSALNVLDNNGAKLGFIAWE
                     GDENIANQESVYINNDLLANPPLNPGNNIFNGTNSITQSSDLYNMDLDIFDIQGNIQV
                     GDQSLDISLTTAQDFVLINTLVTKLNNQLPDATISIDTVEKTCDSKIITVDFTVYNSN
                     STNELPAGTPIAIYANDEFIEFSETILPIEIDGSWSSQITITLPENIPDTFTLQFVVD
                     DSGQGIGHVAELIETNNSFSVEMTLIKTPTFNALEPITSCNEGFTSGTFDFSNYQELV
                     KSIPSDAVHFYETFENATDNVNPILTPSNYSANATPKEIFIRIENENCFSITSFLLLT
                     KNCPPTVYNYISANDDTINDVFTIEGLRNIFLEFKIEIYNRWGRLLWTGNQNTEDWNG
                     YVKDGISIKKATDGTYFYLIFLNDIDYPQPLTGFLYINH"
     gene            2296..3105
                     /locus_tag="PK218_06325"
     CDS             2296..3105
                     /locus_tag="PK218_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPW98157.1"
                     /translation="MKQITSSQNPFIKSLILLQEKAKARKQSGMFLIEGIREIELAIK
                     GDYTLITVLFVPELLAEKNKNAVILSTIEGKSAEIIEISKEAYHKLAYRDTTEGIIAV
                     AKTKQNVLSELILPENPLILVLESIEKPGNIGAMLRTCDAAKVDAILIADIKTDLYNP
                     NIVRSSVGCLFTNQIATGTTDEIITYLQQNKINFFSATLQNSTSYHTQDFTKPTALVV
                     GTEATGLTKAWREKATQNIIIPMQGEIDSMNVSVAAAILLFEAKRQRGFNL"
     gene            3154..4698
                     /locus_tag="PK218_06330"
     CDS             3154..4698
                     /locus_tag="PK218_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPW98158.1"
                     /translation="MKKITLLVSVLLLSFNIISSQIQEKELDELVQNTMKTFNVPGIA
                     VGIIKDGKLVLAKGYGVSNIKTQQKVDANTLFGIASNSKAFTASALAILVDEGKINWD
                     DKVKKYLPDFKMYNDYVTNEFTIRDLLTHRSGLGLGAGDLMIWPDGHDFTPKDIVKNI
                     QYLKPISGFRAKYDYDNLLYVIAGEVIAKISGKSWNDFIEERLMQPLEMNHSASSWNR
                     LKDTTNVIVPHVPTNGKLEIVPRYTNSIFDAAAGLYTSVNDLSHWLIAQMNDANYNGK
                     QLFSKNQHDETWKSQTILPIRNSYPYSTHFSSYGLGWKLTDINGHLEVSHTGGLDGIV
                     TQSIMYPKEKLGIIILTNQQSGTAFNVISNTIKDSYLGFPKFDHLAYLSKERASKEDD
                     ADKITNEVWAKVNQNLKDKSIKIETKKYVGTYKDNWFGEVTISEKKGKLYFASKRSKR
                     LVGEIFFYNNQNFVVKWNVRSFNADAHVFFDLDADGNAKGFTMHAISPLTDFSYDFQD
                     LSFIKI"
     gene            4762..6981
                     /locus_tag="PK218_06335"
     CDS             4762..6981
                     /locus_tag="PK218_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPW98159.1"
                     /translation="MTEIDIEEENKAIAREYKELLRISYQTLSTEDKKLIRKAFDVAV
                     DAHKDQRRKSGEAYIFHPIGVAKIVASEIGLGASSIAAALMHDVVEDTDVTVEEIEKM
                     FNPKIAHLVEGLTKIAQVKKDMNISMQAENFRKMLLTLNDDVRVILIKIADRLHNMQT
                     MDSMPDYKQVKIASETLYIYAPLAHRLGLYNIKTKLEDLGLKYTEPEVFNDIVSKIKE
                     TKEEQDEYIKLVSDVLKKSLDEEGVQYTIKGRPKSIYSIRRKMAAQNVTFDEVYDKFA
                     LRIIYKSNLHDEKFIAWKIYSVVTDHYRPSPSRLRDWISSPKSTGYEALHITVMGPKG
                     RWVEIQVRSERMDEIAEKGYAAHYKYKNGATEESGLDTWLNLLKEALESSETNAVDFV
                     EDFKMNLYAKEIFVFTPKGEIKSLPKGATSLDFAFSIHSEIGIKTRGTKVNGKLVPLN
                     YELKSGDQIEIITSVNQKPSINWLDYVTTSRAKNKIRNVLNENTKKTAEEGREILTRK
                     LKHLKITLNEGVINELVNFFKLKTSLDLFYRVGIGSIDNQQLKDFAAQKSNAFINFFK
                     TKMKRTPSNNPDDINRQELSNNYDMLVFGAENDKLDYKLSNCCNPIPGDDVFGFITIN
                     EGIKVHKRDCPNAIAMQSNYAYRIMQAKWIDSSQQEFKATLKITGMDTLGLTNELTKV
                     ISNNMHVNIQSISLSGEAGLFKGQVAVIVQNNSILKKLIDNIKKIDGVDKVTRVYTN"
     gene            7062..7517
                     /locus_tag="PK218_06340"
     CDS             7062..7517
                     /locus_tag="PK218_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPW98160.1"
                     /translation="MTLIASDNSTNQETVKAVFIAYLENKGHRKTPERYAILQEIYDS
                     EEHFDIEQLYIKMKNKNYRVSRATLYNTIELLLDCGLVRKHQFGQNQAHYEKSYFDKQ
                     HDHIIMTDTGEVIEFCDPRIQTIKKTIEEIFGIDIHNHSLYFYGTKKPE"
     gene            7495..7740
                     /locus_tag="PK218_06345"
     CDS             7495..7740
                     /locus_tag="PK218_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98161.1"
                     /translation="MEPKNPNNTPLTSKQYFVKTFVFVFVFYIVYKAVYQAMSGIDTI
                     TVDFIVKTLGLGFVIAAILGGINYFVKLDFFTKKTKK"
     gene            7774..9045
                     /locus_tag="PK218_06350"
     CDS             7774..9045
                     /locus_tag="PK218_06350"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPW98162.1"
                     /translation="MTVDLLLGLQWGDEGKGKIVDVLTSKYDIIARFQGGPNAGHTLE
                     FDGIKHVLRTIPSGIFHSNNVNVIGNGVVIDPVVFKGEIDGLEKFNLNLKSKLFISRK
                     AHLILPTHRLLDAASEAAKGKAKIGSTLKGIGPTYMDKTGRNGLRVGDIELADFKERY
                     RALADKHEAMIAFYNVDIQYNLEELEKEFFEAIKDLQKLQFIDSEEYLAQAQKGGKSI
                     LCEGAQGSLLDVDFGTYPFVTSSNTTAAGACTGLGIAPNKIKEVFGIFKAYTTRVGSG
                     PFPTEDFGNDGDIMAQVGNEFGSVTGRKRRCGWLDLVALKYAIQVNGVTQLMMMKGDV
                     LSGFDTLKVCTAYNYNGEVIHHLPYNIEEENVTPIYTEIKGWKADLTGMTTYDQLPKE
                     LKDYISMIEAAVEVPISIVSVGPDRKQTITR"
     gene            complement(9100..9312)
                     /locus_tag="PK218_06355"
     CDS             complement(9100..9312)
                     /locus_tag="PK218_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98163.1"
                     /translation="MSIIFEAYGSIVEQHGRCIEANGCIVDANGSNSEINGHFVERHG
                     SLVEGYKALKMLKSYFSHAFIKTYQA"
     gene            9436..10125
                     /locus_tag="PK218_06360"
     CDS             9436..10125
                     /locus_tag="PK218_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98164.1"
                     /translation="MKKKEAVRLYKFSDATLVTKSLEKIAYMKRDETEFDKYNITAVK
                     REALKSEVEAFSELETDVEALQDQVERTELKDKKAEEIRVNVREVMSSVELVYAVDSA
                     KYKKFGTESLSRQTDSELLITAKRVVRVGTEMLPVLSDHGLTAIELAKITDLANEFMD
                     LIVDQHLEIGERDIKQEDRVEAGNAIYSILIKYTNLGQAIWIDKDTAKHNDYVMYNTY
                     TGEAPENGDTV"
     gene            complement(10174..12051)
                     /locus_tag="PK218_06365"
     CDS             complement(10174..12051)
                     /locus_tag="PK218_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HPW98165.1"
                     /translation="MKKHAQLLLVVTILSASFTQAQNKPNMTQVLPESKPKKAVVYQV
                     FTRLFGNKNTTNKPWGTIEENGVGKFNDFTDKALQEIKKLGVTHVWFTGVPHHDVIRD
                     YTKYGISNDDPDVVKGRAGSPYAVKDYYNVNPDLAVNPAKRLQEFEALIKRAHANGLK
                     VIIDIVPNHIARNYHSLSNPKGVKDFGADDDTSVEYKRNNNFYYIPGKAFEVPTSDTY
                     KPLNGEKNPLIDGKFKEFPAKWTGNGSREAKPDINDWYETVKVNYGIKPDGSKDFPEL
                     PEGFDKKDFKAHYEYWQGKDVPDSWKKFRDIALYWTKKGVDGFRYDMAEMVPYEFWSY
                     MNAAIKMQDSDAFLLAEVYNPKEYRNYINLGLMDYLYDKVETYDHLKAVIQGNALPDA
                     LSDIQSNMIDIEHHMLHFLDNHDEQRLASAAFAGTPEKGKPLMVISATISTSPTMIYF
                     GQEVGEPGNEDAGFGKPTRTSIFDYIGVPHHQRWMNGGKFDGGQLTESEKALRDFYAR
                     LLNFTIKSSAMMGKYQEIQTVNRNETKEYDPGLYSFVRWDNKEKLIIIANCSWVTTSH
                     VDLIVPEDIIEKWNLNDGDYKVTDQLYNHEISTLEVKGGRGIIKVKIKPSESFIFKLQ
                     E"
     gene            12223..13827
                     /locus_tag="PK218_06370"
     CDS             12223..13827
                     /locus_tag="PK218_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HPW98166.1"
                     /translation="MLCFVNDLEAQKTSKINIENADFADRDQDEIPDAVLLRGNVKLR
                     HEGVLFTCNKAYFFEKENYLKAFGEVQMVQGDTLFLNSKYAEYNGEVKQAFATGNVIM
                     RSPESTLVTDTINFDRNTQEAFYNTNGTITSKENTLKSKSGRYYANEKKFRFLTGVTI
                     TNPKYVIKSNHLDYYTNSGHSYLFGPSTITSESNYIYTEKGFYDTKKNLAHFLRKSYI
                     KYKDRRIEGDSLYYDRNREFASATNNVKITDSINNGVVKGHYAEMYKLKDSMYVTKRA
                     VAINLVENDSVYIHGKKLMVTGKPGNRIVRAFNNVRFYKKDMSGKCDSLYSDQKTGLT
                     KLIKNPILWNFENQMTGDEMHLIGNNQTEKLDSLKVLNNAFIVSKDTLGTGYNQVKGQ
                     NLYGKFEDSKLREVDIIKNTEVIYFMRNDQQELIGINKSVCSKINILFDGNTIETITN
                     FNQAEGEIYPEKDLPENARKLRGFIWRGDERIKTKDDIFPEEENQLDAKIQAEKKLEM
                     EKENVPLEPRKETLDYDKKNPKPEIK"
     gene            13860..14087
                     /locus_tag="PK218_06375"
     CDS             13860..14087
                     /locus_tag="PK218_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98167.1"
                     /translation="MAQHICPKCKQETFNWYIDEEIPSITIWSCSNCNIQIFEDDRDE
                     VYCEKCQDTTKTFLKNEEEEFWWCSVCATTQ"
     gene            14101..15288
                     /locus_tag="PK218_06380"
     CDS             14101..15288
                     /locus_tag="PK218_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPW98168.1"
                     /translation="MKEDFLKYQAQTSPYPLGLEISHAVGSYIFDTNDKKHLDFVAGV
                     SACSLGHQHPRVNQAIKDQLDKYSHVMVYGEYAQHPAVQYCKLLVEHLPKSLNKIYLV
                     NSGTEACEGALKLVRRVTGRSQLISCHNAYHGNTMGSMSVMGFEERKQIFRPLIPDVD
                     FITFNNEEDISKITTKTAGIILESIQGGAGFIEPKNDFLAKIKKRCEEVGALMIVDEI
                     QPGFGRTGKLFGFENYNVVPDVIIIGKGMAGGMPVGGFIANEKHMDLLSHDPKLGHIT
                     TFGGHPVIAAACLATLEEILEKDYAKQSLEKEKLFRSLLVHPLIEEIRGKGLMLAAMT
                     KNAEITNQVIFKCQDKGLILFWLLFEPSAIRITPPLTISDDEIKEGCAIIIEAMNEIL
                     NEN"
BASE COUNT         5300 a         2807 c         2737 g         4480 t
ORIGIN      
        1 aatcagaaaa caataaaagt attatactga ctaaaaaagt agctatcaac aagcaaaaaa
       61 ctattgttga tattgttttt tttaatctaa gctgctcttc ttcaaattca gcaacatatt
      121 ttacaattcc attttgcaat ccaagagttg aaatactctc tattgaagtt ataaaattcc
      181 tgaaattacc aacaattgcc attccagatg gtccaataaa aaccgcaata atttttgaag
      241 aaacaaaccc tacaccaatt ttaattaata cactaatact gtttaaagaa gtgactttaa
      301 atagttcttt agaagttatt ttttttataa actgcaagta attattaatt tgaccgaaag
      361 taaacattta ttttcaaata cactaaaacg acaaataatc tatttataaa aataaattct
      421 taaatttaat gactatttta acactgtgaa aagaaaacga attttatcgt tatttattgt
      481 attacttggc atttttttaa aaggaaatgc acaagatatt gaattgtatc aacaatttaa
      541 tggtcgatat gattttactt ttattggaaa cacgatgaat actgaagcta atggtttttt
      601 tacgccttgt tatgctttga ccagttctag tgccacttta aatttaaacg ttaatgatca
      661 aatagaaaaa gtgtttctat attgggctgg ttcaggaact ggcgattttg atattcaatt
      721 aaatgatgtt gacatcagtt cacaaattaa ttttacagct attcaaaact ctagtttgaa
      781 tcattttttt agtgcatttg cagatgttac acaacaagta cttactactg gaaatggcat
      841 ttatacagtt tcagaattgg acataaatcc ttttatcgat tcggatacat attgtaataa
      901 tgcaacaaat tttgcgggtt gggcaatgtt aattgtttat aaaaacacta ctttacccat
      961 caatcaaatc aatgtttatc acggtttaca agctttgtcc gcagcggtag taaatgttca
     1021 aacacaaata accattacac tttctgcact aaatgtatta gataataatg gtgcaaaatt
     1081 aggttttatt gcttgggaag gtgatgaaaa tattgccaat caagaatcgg tttatattaa
     1141 taatgatttg cttgcaaatc caccattgaa tccaggaaat aatattttta atggtaccaa
     1201 cagcatcacc caatcatccg atttatataa tatggatttg gatatttttg atattcaagg
     1261 aaacattcag gttggagatc aaagtctcga tattagttta actacagcac aagactttgt
     1321 attaatcaac acattggtaa ccaaactaaa caaccaattg cccgatgcaa caattagtat
     1381 agatactgtt gaaaaaacat gtgattcgaa aattattacg gttgatttta cagtttataa
     1441 ttcaaattct accaatgaat tgccagcagg aacaccaatt gccatttatg ctaatgacga
     1501 atttatagaa ttttcagaaa caattctacc tattgaaatt gacggaagtt ggagtagtca
     1561 aataacaata acattgcccg aaaacattcc tgatacgttt acattgcaat ttgttgtaga
     1621 tgatagcggt caaggaatag gtcatgtagc cgaattgatt gaaacaaaca actctttttc
     1681 agttgaaatg acattaatca agacaccaac atttaatgct ttagaaccca ttacatcatg
     1741 caatgaaggt tttacaagtg gcacttttga tttttctaat tatcaagaat tagttaaatc
     1801 cattccaagt gatgcagttc atttttatga aacattcgaa aatgcaactg ataatgtaaa
     1861 tccaatttta acaccttcta attatagtgc aaatgctaca ccaaaagaaa tttttattcg
     1921 aatcgaaaat gaaaattgct tcagcatcac ttcatttctg ttgctaacaa aaaattgtcc
     1981 gccaacggtt tacaattata tttcagctaa tgatgacact ataaacgatg tcttcactat
     2041 tgaaggttta cgaaatattt ttcttgaatt taaaatcgaa atttacaatc gttggggaag
     2101 attgctttgg acaggaaatc aaaataccga agattggaat ggttatgtaa aagacggtat
     2161 tagtataaaa aaagctaccg acggcaccta tttttactta atttttctaa atgacatcga
     2221 ctatcctcaa cctttaactg gttttttata catcaatcat taaaataatt taactttgag
     2281 gtcttaattt tcagaatgaa acaaatcact tcttcccaaa atccgtttat aaaatcattg
     2341 attttattgc aagaaaaagc taaagctcga aaacaatcgg gcatgttttt aattgaagga
     2401 attcgtgaaa ttgaattggc aattaaaggc gattatacat tgattactgt tttattcgtt
     2461 ccagaacttt tagctgaaaa aaacaaaaat gccgtcatcc ttagcacaat cgaaggaaaa
     2521 tcagcagaaa taattgaaat ctcaaaagaa gcatatcaca aattagcata tcgagacact
     2581 actgaaggaa ttattgccgt tgcaaaaaca aaacaaaacg tactttcaga attaatactt
     2641 ccagaaaacc cactgatttt agtattggaa agcattgaaa aaccaggaaa tattggtgcc
     2701 atgttacgca cttgcgatgc tgcaaaagtt gacgccattt taattgctga catcaaaacc
     2761 gatttataca atccaaatat tgttcggtca agcgtgggct gtttatttac caatcaaatt
     2821 gcaacgggta caactgatga aataattact tatttacagc aaaacaaaat taatttcttt
     2881 agtgcaacgc tccaaaattc aacctcatat cacactcaag atttcaccaa accaacagca
     2941 ttagtagtgg gtacagaagc aacaggatta acaaaagctt ggcgagaaaa agcaacacaa
     3001 aacatcatca ttccaatgca aggtgaaatt gacagcatga atgtttcagt ggcagcagcc
     3061 attttattat ttgaagccaa aagacaaaga gggtttaatt tgtaattttg gcaattagac
     3121 tttaaagatt agagaattat atacatttat caaatgaaaa aaataacact tttagtttcc
     3181 gtattattac tgtcttttaa cattatttct tctcaaattc aagagaaaga attagacgaa
     3241 ttggtgcaaa ataccatgaa aacgttcaat gttccgggta ttgccgtagg aatcattaaa
     3301 gatggaaaac tagttttagc aaaaggttat ggcgtttcca atataaaaac tcaacaaaaa
     3361 gtggatgcca atacgttgtt cggaattgct tcaaacagta aagcttttac agcatcagct
     3421 ttagcaattt tggttgatga aggcaaaata aactgggacg ataaagtcaa aaaatacttg
     3481 cccgatttta aaatgtataa cgattatgta accaatgaat ttactattcg tgatctatta
     3541 acgcacagaa gtggtttagg tcttggggct ggcgacttaa tgatttggcc agatggtcac
     3601 gacttcacac cgaaagacat cgttaaaaac atccaatacc ttaaacccat ttctggtttc
     3661 cgtgctaaat atgattacga caacttatta tatgtaatcg caggtgaagt aattgctaaa
     3721 atttcaggta aatcatggaa tgattttatt gaagaacgtt tgatgcaacc tttagaaatg
     3781 aaccatagcg cttcgtcttg gaatagatta aaagacacca ctaacgtaat cgttccgcat
     3841 gttccgacaa atggcaagtt agaaatcgtt cctcgttata ccaattcaat ttttgatgcc
     3901 gcagctggat tatatacatc tgtaaacgat ttaagccatt ggttaattgc ccaaatgaac
     3961 gatgcaaact acaacggaaa acaattattt agcaaaaatc aacacgacga aacttggaaa
     4021 tcacaaacca ttttacccat acgaaactct tatccctatt caactcattt tagttcttat
     4081 ggtttaggtt ggaaattaac cgatattaac ggacatttag aagtatctca cactggcgga
     4141 ttagacggaa tcgttacgca aagcatcatg tatccaaaag aaaaattagg catcattata
     4201 ttgaccaacc aacaaagtgg taccgctttc aacgtgatat caaacaccat caaagacagc
     4261 tatctgggtt ttcctaagtt tgaccatctt gcctacttga gtaaagaacg tgcctctaaa
     4321 gaagacgatg ccgataaaat tactaacgaa gtttgggcta aagtaaacca aaacttgaaa
     4381 gacaaatcta ttaaaataga gaccaaaaaa tatgttggaa cttataaaga taattggttt
     4441 ggagaagtta ccatttcgga gaaaaaagga aaactatatt ttgcttccaa acgttcaaaa
     4501 cgattagtag gcgaaatatt tttctacaac aatcaaaact ttgttgtgaa atggaatgta
     4561 cgcagcttca acgctgatgc acatgtcttt tttgatttgg atgctgatgg taacgcaaaa
     4621 ggatttacca tgcatgccat ctcgccatta accgacttta gctacgattt tcaagactta
     4681 agcttcatca aaatataatt cagagtgcct attgtgcatt tcaatactta tgcttaactt
     4741 tagtaattca tttgaatacg tatgactgaa attgatatcg aagaagaaaa caaagccatt
     4801 gcccgagaat acaaagaatt actccgaata agctaccaaa ccctttctac agaagacaaa
     4861 aagctgattc gtaaagcatt tgacgtggcg gttgatgccc acaaagacca aagacgaaaa
     4921 tcgggtgaag cttatatctt tcaccctatt ggtgttgcaa aaatagttgc ttccgaaatt
     4981 ggtctcggcg cctctagtat tgctgctgcc ttgatgcacg acgttgttga agacaccgat
     5041 gttacggtag aggagattga aaaaatgttc aatccgaaaa ttgctcattt agtcgaagga
     5101 ttgaccaaaa tcgctcaggt taaaaaagac atgaacatct ctatgcaagc cgaaaacttc
     5161 cgcaagatgc ttttgacctt gaacgacgac gttcgcgtca tcttaatcaa gattgcagac
     5221 cgactacaca acatgcaaac catggattcg atgcccgact ataagcaagt caaaattgca
     5281 tccgaaacct tatacatcta cgcgccgctt gcacaccgac taggcttgta taacatcaaa
     5341 acaaagctag aagatttagg tttaaaatat accgaacccg aagttttcaa cgatattgta
     5401 agcaagataa aagaaactaa ggaagaacaa gacgaataca tcaaattagt atccgacgtt
     5461 ttaaaaaaat cattggacga agaaggtgta caatatacca tcaaaggaag acccaaatcc
     5521 atctattcca tacgccgtaa aatggctgcc caaaacgtga cttttgacga agtatatgat
     5581 aaatttgcgc tccgaatcat ctataaatcc aatttacacg acgaaaaatt cattgcatgg
     5641 aaaatatatt ctgttgtaac cgatcattac cgtccgtcac ctagtagatt gcgcgattgg
     5701 atttcttctc caaaatctac aggctacgaa gcacttcaca tcaccgtgat gggaccaaaa
     5761 ggtcgctggg tagaaattca agtgcgcagc gaacgcatgg acgaaattgc cgaaaaaggt
     5821 tatgccgctc actataaata caaaaatggt gctaccgaag aaagcggact cgacacttgg
     5881 ctcaacttat taaaagaagc actcgaaagt tcagaaacca atgccgttga ttttgtagaa
     5941 gacttcaaga tgaacttgta tgccaaagaa atcttcgttt ttacacccaa aggagaaata
     6001 aaatccttgc ccaaaggtgc cacttcactc gattttgctt ttagcattca ctccgaaatt
     6061 ggcataaaaa cccgcggaac caaagtaaac ggcaagctcg tgcctttaaa ctacgaatta
     6121 aagagcggag accaaattga aatcatcaca tcggtcaacc agaagccgtc tatcaactgg
     6181 ttagactacg taaccacgtc gagagccaag aacaagatta gaaacgttct gaatgaaaac
     6241 accaaaaaga ccgctgaaga aggaagagaa atccttaccc gaaaattaaa gcatttaaaa
     6301 atcactttaa acgaaggagt catcaacgaa ttggtaaatt tcttcaaatt aaagacgagt
     6361 ttagacctat tttatcgcgt aggcattggc tctatcgaca atcagcaact aaaagacttc
     6421 gccgcacaaa aaagcaacgc gtttatcaat ttcttcaaaa cgaaaatgaa gcgtactccg
     6481 tcgaataatc cggatgacat caaccgacaa gagcttagca acaactacga catgcttgta
     6541 tttggcgcag aaaacgataa actcgactac aagctttcca actgctgcaa ccctattcct
     6601 ggagacgacg tttttggctt tatcaccatc aatgaaggca tcaaagtaca caaaagagac
     6661 tgcccaaacg caatagccat gcaatccaac tatgcttata gaatcatgca agcgaaatgg
     6721 atagactcta gccaacaaga atttaaagca acactgaaaa ttaccggaat ggacacgcta
     6781 ggactaacca acgagctgac taaagtgatt tccaacaaca tgcatgtcaa catacaaagt
     6841 atatcactga gcggagaagc cggattgttt aaaggtcaag tggcggttat tgtacaaaac
     6901 aacagcatcc tcaaaaaact catcgacaac attaaaaaaa ttgacggagt agataaagta
     6961 acccgcgttt ataccaacta agcgttcatt atcaacaatt cgttcaaaac tttagagctt
     7021 gaaactttct taattttaat tgaataactt acctttgcct tatgacactt atagcatcag
     7081 acaatagcac aaatcaagag accgtaaagg ctgtttttat tgcctatctt gaaaacaaag
     7141 gacaccgaaa aactcccgaa cgctatgcca tccttcaaga gatttacgac agcgaagagc
     7201 attttgacat tgagcagctg tatatcaaaa tgaagaataa aaactaccgc gtttctcgtg
     7261 ctacgttata caataccatc gaattattgc tcgattgtgg gttggtgcgc aaacatcagt
     7321 ttggacaaaa ccaagcgcac tatgaaaaat cgtattttga caaacaacac gaccatatta
     7381 tcatgacgga caccggcgaa gtgattgagt tttgcgaccc aaggattcaa acgataaaaa
     7441 aaaccataga agagatcttt ggtatcgata ttcacaacca ttcactctat ttttatggaa
     7501 ccaaaaaacc cgaataatac accattaacc agtaaacaat actttgtaaa aacctttgta
     7561 ttcgttttcg ttttctatat tgtttacaaa gccgtctatc aagccatgtc gggcatagat
     7621 accataactg ttgactttat cgtcaaaaca ttaggactag gatttgtcat tgccgccatt
     7681 cttggaggca tcaattactt cgtaaaactt gatttcttta cgaaaaaaac aaaaaaataa
     7741 ctacacaaca catacatcaa acaacaacac acaatgacag tagatttatt actaggatta
     7801 caatggggag acgaaggaaa aggtaaaatc gttgacgtat taacctcgaa atacgacatc
     7861 atcgcgcgct ttcaaggcgg accaaacgca ggacataccc tagaatttga cggcatcaaa
     7921 cacgtattgc gcaccattcc gtcgggaata tttcatagca acaacgtaaa cgtaattgga
     7981 aacggtgtcg taatagaccc agtggttttt aaaggcgaaa ttgatggctt agaaaaattt
     8041 aacctcaatt tgaaatcgaa attattcatt tcgagaaaag ctcacctcat tttacctacg
     8101 cacagattgc ttgatgcagc atcagaagcc gccaaaggca aagccaaaat tggttcaacc
     8161 ttgaaaggta ttggaccaac ctatatggac aaaaccggaa gaaacggact aagagtgggc
     8221 gatatagaac tagccgattt taaagaaaga taccgcgcat tggccgacaa acacgaagcc
     8281 atgattgcgt tttacaacgt tgacattcaa tacaacctag aagaattaga gaaagaattc
     8341 tttgaagcca tcaaagattt gcaaaaacta caatttatag acagcgaaga atacttagca
     8401 caagcccaaa aaggcggaaa atctattttg tgcgaaggcg ctcaaggttc gttgctcgat
     8461 gttgattttg gaacctatcc gtttgtaaca tcctccaaca ccactgctgc cggagcttgc
     8521 accggactag ggattgcccc aaacaagatt aaagaagtat tcggaatctt caaagcctat
     8581 accacacgcg taggttcggg accgtttcct accgaagatt ttggtaatga tggcgacatc
     8641 atggcgcaag taggcaacga atttggctca gtaaccggaa gaaaacgtag atgtggctgg
     8701 ttagacctgg ttgctctaaa atatgccatc caagtaaatg gtgtaaccca attaatgatg
     8761 atgaaaggcg acgtactatc ggggtttgac acgctaaaag tgtgtactgc ctacaactat
     8821 aatggtgaag ttattcatca cctaccttat aatatagaag aagaaaacgt aaccccgata
     8881 tataccgaaa tcaaaggttg gaaagccgat ttgaccggga tgactaccta tgaccaactg
     8941 ccaaaagaat taaaagacta catcagcatg attgaagccg ccgtagaagt gccaatcagc
     9001 atcgtatcgg ttggtcctga caggaaacaa actattacac gataaagaaa tagtaactta
     9061 tttatagaaa aaaacctttt actgagtaaa aggttttttt tatgcctgat atgttttgat
     9121 gaaagcatgg gaaaagtaac ttttcagcat tttcaaggct ttgtagcctt caacaaggct
     9181 tccgtgtctt tcaacaaagt gtccgttgat ttcactatta cttccgttgg catcaacaat
     9241 gcatccgtta gcttcaatac atcttccgtg ttgttcaaca atgcttccgt aggcttcgaa
     9301 aataatactc aaagcttcaa aatattttcc gtagtatttt ttttcatttt ttttgccttc
     9361 agagatacaa cttataaaac ttttttttaa gtttgattat tgtaactgaa tactcactta
     9421 aaaatcaacc ataccatgaa gaaaaaagaa gccgttcgcc tttacaaatt ctctgatgcc
     9481 actttggtaa ctaagtctct tgaaaaaatt gcttatatga aaagagacga aactgaattt
     9541 gataaataca atattactgc tgtaaaaaga gaagctttaa aatccgaagt tgaagctttt
     9601 tcggaacttg aaactgatgt tgaagccttg caagaccaag tagaacgaac tgaattgaag
     9661 gataaaaaag ccgaagaaat tcgagtaaac gttcgcgaag taatgtcgag cgtagaatta
     9721 gtatatgctg ttgacagtgc taagtataaa aagtttggca cagagtctct ttctcgtcaa
     9781 actgattctg aattgttaat cactgccaaa agagtggttc gcgtaggcac agaaatgctt
     9841 ccggtgcttt ctgaccatgg tttaacagct attgaattgg caaaaattac cgatttggcc
     9901 aatgaattta tggatttaat tgttgaccaa cacttagaaa ttggcgaaag agatattaaa
     9961 caagaagacc gcgttgaagc tggcaatgct atttatagta ttttgataaa atataccaat
    10021 cttgggcaag ccatttggat tgataaagat accgctaagc ataatgacta tgtgatgtat
    10081 aatacataca ctggcgaagc tcccgaaaat ggcgacactg tctaatcgta ctaaaaaacc
    10141 ctttctgctc aggaaagggt tttgttttta ctgttattct tgtagcttaa agataaaact
    10201 ttcgcttggt ttgattttta ctttgataat gccgcgtccg ccttttactt ctaatgtaga
    10261 aatttcgtgg ttatacaatt ggtcggttac tttatagtcg ccgtcattca agttccattt
    10321 ttcgataata tcttcgggca caatcaaatc aacatgactg gtggtaaccc acgaacaatt
    10381 ggcaataatg atgagttttt ctttgttatc ccagcgtaca aacgagtaca atcctgggtc
    10441 gtattctttg gtttcgtttc tattcacggt ttgtatttct tgatacttgc ccatcatcgc
    10501 actgctcttg atggtgaagt ttaataatcg ggcgtagaaa tctcttagcg ctttttcgct
    10561 ttcggttaat tgaccgccgt caaattttcc gccgttcatc cagcgttgat ggtgtggtac
    10621 gccaatataa tcaaagattg aagttctagt tggcttgcca aaaccagcat cttcgttgcc
    10681 gggttcgcct acttcttgcc caaaatatat catagttggc gatgtgctga tggttgccga
    10741 gatgaccatt agtggttttc ctttttcggg tgttcctgca aaagcggcac ttgccaatcg
    10801 ttgttcgtca tgattgtcta agaaatgcaa catgtgatgt tcaatgtcaa tcatattgct
    10861 ttgaatatca gataaagcat caggcaatgc attgccttgt attacggctt ttaaatgatc
    10921 ataagtctca accttatcgt ataagtagtc cattaatcct aggttgatgt agttgcggta
    10981 ttcttttggg ttataaactt cggctaaaag aaaggcatca gagtcttgca tcttgatggc
    11041 agcattcata tagctccaaa attcgtaagg caccatctct gccatgtcgt atcggaagcc
    11101 gtcaacgcct ttttttgtcc aatacaaagc aatatcgcgg aattttttcc agctatcggg
    11161 tacgtctttt ccttgccagt actcgtagtg tgctttgaaa tcttttttat caaaaccttc
    11221 gggtagttcg gggaaatctt tgctaccatc gggtttgatg ccgtagttta cttttacggt
    11281 ttcataccaa tcgttgatgt ctggtttggc ttcgcgcgaa ccgtttccgg tccattttgc
    11341 aggaaactct ttgaatttac catcaatcaa aggatttttt tcgccattca aaggtttgta
    11401 agtatcagaa gttggtactt caaaagcttt accaggtatg taataaaagt tgttgttgcg
    11461 tttgtattct accgaagtgt catcgtctgc tccgaagtct ttcactcctt ttgggttgct
    11521 caaactatga taattacgag caatgtgatt aggaacaata tcgatgatga cttttagtcc
    11581 gttggcatgc gctcttttaa tcagtgcttc aaattcttgc agtcttttag caggattaac
    11641 cgccaaatcg ggattaacgt tgtagtaatc tttcacagca tacgggcttc cggcacgtcc
    11701 tttcaccaca tcgggatcat cattcgaaat gccatatttg gtataatctc tgataacatc
    11761 atgatgcggc acgccagtaa accaaacgtg ggttacgcct agttttttga tttcctgcaa
    11821 ggctttgtcg gtaaaatcgt tgaacttgcc tacaccgttt tcttcaatgg ttccccatgg
    11881 tttgttggtg gtgtttttgt tcccaaacaa tcgggtgaat acttgatata ctacggcttt
    11941 ttttggcttg ctttcgggta atacttgggt catgttgggt ttgttttggg cttgtgtgaa
    12001 gcttgcagat agaatagtta ctactaatag gagttgtgcg tgttttttca taatacattc
    12061 gttgacgaca ctttgattta aaaagtcgta gtgacaaata taaagaattg attgaaagtg
    12121 gaaacataaa aaaaccttaa ttgcgtatcc tattggttct aaattcctaa atttgacaaa
    12181 aatttaaacc atttgaagaa gttactttta cttttcggat ttatactttg ctttgtcaat
    12241 gatctagaag ctcaaaaaac ttctaaaatt aacattgaaa atgctgattt tgctgataga
    12301 gatcaagacg aaatacctga tgcagttctt ttgcgcggaa atgttaagct tagacacgaa
    12361 ggtgttttat ttacttgcaa caaagcctat ttttttgaaa aagaaaacta tctaaaagct
    12421 tttggagaag tgcaaatggt acagggtgat actcttttct taaacagtaa atatgctgaa
    12481 tataatggag aagtcaaaca agcatttgca acaggaaatg ttattatgcg ttctccagaa
    12541 tctacgttgg ttactgacac tattaacttt gacagaaaca ctcaagaagc tttttacaat
    12601 actaacggaa cgataactag taaagaaaac acattaaaaa gtaaatcagg acgctattat
    12661 gccaatgaaa aaaagtttcg ctttttgacc ggagtaacca ttaccaaccc aaaatatgtc
    12721 atcaagtcca atcatttaga ttattacact aattctggac attcctatct ttttgggcca
    12781 tcaacaataa ctagcgaatc caattatatc tataccgaaa aaggatttta tgataccaag
    12841 aaaaacttgg cgcattttct acgaaaatcc tacatcaaat acaaagacag gagaattgaa
    12901 ggcgacagtc tatattatga ccgcaaccga gagtttgctt cggctaccaa taatgtaaag
    12961 attacagact caataaacaa tggtgttgtc aaaggtcatt atgcagagat gtacaagctt
    13021 aaagattcta tgtatgtaac caaaagagct gttgctatta acttagtaga aaacgattcg
    13081 gtctatattc acggaaaaaa attgatggtt acaggaaaac ctggaaatcg aattgtaaga
    13141 gcttttaaca acgtacgttt ttacaaaaaa gacatgagtg gaaagtgtga ttccttatat
    13201 tccgaccaga aaacaggact caccaaattg ataaaaaatc caatcctttg gaactttgaa
    13261 aaccaaatga ccggtgatga gatgcatctt attggtaata accaaaccga aaagctagat
    13321 tcgctcaaag tactcaataa tgctttcatt gtttccaaag acacacttgg cactggttac
    13381 aatcaagtga aaggacaaaa tttatacggc aaatttgaag acagcaaact ccgagaagtt
    13441 gacattatca aaaacaccga agtgatttat tttatgcgaa atgaccagca agaattgatt
    13501 ggcataaaca aaagtgtttg cagtaaaata aacatactgt tcgacggaaa caccatcgaa
    13561 accatcacga attttaacca ggcagaaggt gaaatttacc ccgaaaaaga tttgcccgag
    13621 aacgcaagga aacttcgagg ctttatatgg cgtggcgatg aacgtatcaa aacaaaagac
    13681 gatatttttc ctgaagaaga aaaccaattg gatgctaaaa ttcaagccga aaagaaacta
    13741 gaaatggaaa aagaaaatgt tccgctggaa cctagaaaag aaacattgga ttatgataaa
    13801 aaaaatccca agcctgaaat caaatagcgt ttcaaaccct acaattaatt tacccaaaaa
    13861 tggcacagca tatttgtcca aaatgtaaac aagaaacttt taattggtat atagacgaag
    13921 aaattccgtc aataacgatt tggagttgct cgaactgtaa tatacaaatc tttgaagatg
    13981 atagagatga agtatattgc gaaaaatgcc aggatactac aaaaaccttt ttaaagaacg
    14041 aagaagaaga attttggtgg tgttctgtat gtgccactac tcagtaaaac tataatttca
    14101 ttgaaagaag actttttaaa ataccaagct caaacatctc cctatccttt agggttggaa
    14161 atctcacacg ctgttggctc ctatatattt gacacaaacg ataaaaaaca tttagatttt
    14221 gttgcgggtg tttctgcctg ttcactcgga catcaacatc caagagtcaa tcaagccatc
    14281 aaagaccaat tagacaaata ctcgcatgtg atggtctatg gcgaatatgc acaacatcca
    14341 gctgtgcaat actgtaaatt attggtagaa catcttccaa aatctttaaa taaaatctat
    14401 cttgttaact cgggaactga agcttgcgaa ggtgccttaa aattagtacg aagagttact
    14461 ggtagaagtc aattgatttc ctgtcacaat gcttatcatg gcaatactat gggttcgatg
    14521 agcgttatgg gttttgaaga acgtaaacaa atctttcgtc cattaattcc agatgttgat
    14581 ttcattactt ttaataacga agaagatatt tctaaaataa caaccaaaac tgctggaata
    14641 attctagaga gtattcaagg tggagcgggt tttattgaac caaaaaatga ttttttggca
    14701 aaaataaaaa aacgctgtga agaagtgggc gcgctcatga tagttgacga aatacaacca
    14761 ggctttggaa gaactggaaa attattcggt tttgaaaact ataacgtcgt tcccgacgta
    14821 attattattg gaaaaggaat ggctggcgga atgcctgtcg gtggatttat tgccaacgaa
    14881 aaacacatgg atttattgag ccacgaccca aaattaggac acatcactac ttttggcggc
    14941 catcctgtca ttgcggcagc ctgtttagca accttagaag aaatactaga aaaagactat
    15001 gctaagcaat ctttagaaaa agaaaagtta tttcgatcgc ttttggtaca tcctttgata
    15061 gaagaaataa gaggaaaagg attaatgctt gcagcaatga ctaaaaatgc agaaataacc
    15121 aaccaagtta tttttaaatg tcaggataaa ggtttaattt tattttggtt attgtttgaa
    15181 ccaagtgcta ttagaataac gccgccatta accatttctg atgatgaaat aaaagaaggt
    15241 tgtgccatta ttattgaagc gatgaatgaa attttgaatg aaaattaata attaatttat
    15301 ttttgatgta gcggtttaac ttta
//
LOCUS       DAOOBH010000047        15191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5093_length_15191_cov_6.149247, whole genome shotgun sequence.
ACCESSION   DAOOBH010000047 DAOOBH010000000
VERSION     DAOOBH010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5093_length_15191_cov_6.149247"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1748
                     /locus_tag="PK218_06385"
     CDS             174..1748
                     /locus_tag="PK218_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98169.1"
                     /translation="MKKLYSLLLLFFISGNATNYYVSQLTGIMSNDGLTPETAFLTLQ
                     QAHDISQPGDVIFIMNGIYSNDNADSDILEIVISGTENQWITYKNYEGHQPVLKLNAN
                     NWQAVNIQGADYIIIDGLKIIGNNDAISLEYALSEQNNFDNAATSGNGIAISIEFDNQ
                     ENKSHHVIIRNCTISKCGGGGIYSYHSDYITIENNTVYECAWYSPYNNSGISMYQNWN
                     SDNSLEIKNIISGNTCYRNECYIPRLIEGVPTIIDGNGIVIDDSRNTQFDSPLGAYLG
                     KTYIINNVIFDNGARGIHSYSSDNIIVSNNTLYKNCQSDDTQEGELTAFDSSAITYIN
                     NIVFPDVNIPPIAIYNIADLIVDYNLWAENSDLAAHFGTNTLIGNPDFVFPSTNPLEA
                     DFHLMPSSIAINSGSSTVAPITDKDGNSRIDNVDLGAYEFQPNLNTIKIQDASFIIYP
                     NPVKESLTIVFSENNSELVEISIYNSLGQSIASSLKAIANHSLTIPLNHLSNGIYYVT
                     ISKNGKQIDTKKIIKI"
     gene            1847..2692
                     /locus_tag="PK218_06390"
     CDS             1847..2692
                     /locus_tag="PK218_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPW98170.1"
                     /translation="MSNTQHLHDFTTQVRRDILRMVHAVNSGHPGGSLGCAEFFTVLY
                     QNILERKPGFDMDGIGEDLFFLSNGHISPVFYSVLARSGYFPVSELATFRKLNTRLQG
                     HPTTHEGLPGVRVASGSLGQGLSVAIGAAQAKKLNKDSHLVYALTGDGELQEGQNWEA
                     IMYASAKKVDNLIATVDLNGQQIDGSTDEVLNMGSIKAKFEAFDWDVLEIKEGNNIEA
                     VLTGMNEAKSRTGKGKPICVLLHTVMGNGVDFMMHTHAWHGKAPNNEQLATGLAQNAE
                     TLGDY"
     gene            2750..3703
                     /locus_tag="PK218_06395"
     CDS             2750..3703
                     /locus_tag="PK218_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPW98171.1"
                     /translation="MKKYENQGSKDTRSGFGAGMTELGQKNENVVALCADLIGSLKFD
                     DFKKNHPERFFQIGIAEANMIGIAAGLTIGGKIPFTGTFANFSTGRVYDQIRQSVAYS
                     DKNVKICASHAGLTLGEDGATHQILEDIGLMKMLPGMTVINTCDYNQTKAATIAIADH
                     HGPVYLRFGRPVVPNFTPTNQTFEIGKAILLNEGTDVTIVATGHLVWEALVAAEALEA
                     KGISAEVINIHTIKPLDDAAILKSVAKTGCVVTAEEHNFLGGLGESVSRVLALNNPKP
                     QEFVAVNDSFGESGTPEQLMEKYKLNNQAIVEAVEKVLKRK"
     gene            complement(3765..5150)
                     /locus_tag="PK218_06400"
     CDS             complement(3765..5150)
                     /locus_tag="PK218_06400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPW98172.1"
                     /translation="MAKFELKLPKMGESVAEATITNWLKNVGDTVNADEAVLEIATDK
                     VDSEVPSEVSGTLTEILFQVNDVVQVGQTIAYIETEVNSAVSTPEVQVNPPVAVAEVT
                     KTFEAARESVAPVVLEKADYSNSDKFFSPLVKNIALQEGVSVGELNSISGSGKEGRVT
                     KEDILTFVAKRNTNGVAKVATIEAPVPVATPQIVEQKVEETPKASPQPAATISKTESV
                     KAPVSVNGGDEIIEMDRMRKLISGYMVASVQTSAHVQSFIEVDVTNIVKWRDKVKTAF
                     EKREGEKLTFTPIFMEAVARALKDFPMMNISVDGDKIIKRKNINLGMAAALPNGNLIV
                     PVIKNADELNIVGMAKAVNDLGNRAKAGKLKPDDTQGGTYTVTNVGTFGSVFGTPIIN
                     QPQVGILALGAIRKVPAVIETPDGDFIGIRQKMFLSHSYDHRVVDGALGGSFVKRVAE
                     YLEAFDENRDY"
     gene            complement(5267..6448)
                     /locus_tag="PK218_06405"
     CDS             complement(5267..6448)
                     /locus_tag="PK218_06405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW98173.1"
                     /translation="MQLSVIILNYNVRYFLEVCVLSVQKAIQNIDAEIIVIDNHSSDD
                     SCAMMKTKFPNIKLIENKENYGFPKGNNIAVNEAKGEFICILNPDTIVAEDTFVKILN
                     FVTLSAVEVPNSQLSAGIIGCKLIDGSGQFLPESKRGIPTPWVAFTKVFGLYKWFPKS
                     SLFNKYYAQHLDKNQTGNVEILVGAFMIMKRELYNEVGGFDENCFMYSDDIDLSYMVL
                     QTGKANYYYHDTTVIHFKGESTVRDEKYMKRFQEAMNYFYRKHFKKSYFFSFMMKMGS
                     IFFSFVKKYQAEKPAKSSPDSYVLISSTLELKDKLSKELQKNIQLATIENGKVVFSQT
                     ISEHEKTEIIFDCSYCSFKEALIFMEQNAKRFTFKFLIKENGYLIGSNSSNDRGEIIF
                     I"
     gene            6576..8438
                     /locus_tag="PK218_06410"
     CDS             6576..8438
                     /locus_tag="PK218_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPW98174.1"
                     /translation="MNYLSVENISKAFGERVLFKDISFGINKDQKIAFIAKNGSGKTC
                     IMKIINGEDEADSGQVVVRKGLNMAFLSQSNNLQDELTIEESIFASDNEILKVIERYE
                     KALENPEDADKYQLAFDEMDSYNAWDFETQFKQILSKLKLDDLKLKVKSLSGGQKKRL
                     SLAIILINRPDLLILDEPTNHLDLEMIEWLESYFAKENITLFMVTHDRFFLERVCNEI
                     IELDNGKLYSYKGNYSYYLEKKEERIAMENSSIDKAQNLFVKELAWMRRQPKARTTKS
                     KSRQDDFYVIKEKAESRRKENVVELEINMERMGSKIIELHKLFKKFKEKTILDNFSYD
                     FQRGERIGIIGKNGTGKSTFLNILTKTMLPDAGKVVVGDTIKIGYYTQSGINPKPGQK
                     VIDIIKEYGEYIPLTKGKIISASQLLERFLFDAKKQYDFVEKLSGGELKRLYLCTVLI
                     QNPNFLILDEPTNDLDIVTLNVLESFLLDYPGCLLVVSHDRYFMDKIVDHLFIFRGEG
                     QIEDFPGNYSDFRSYEDSVEPKNLSNVSTEKTNWKQNNPTNSGLNFNEQKEFNKIERE
                     IKDLEYEKKQIENLFSEGKVADDQIEKKAIELQKVIERLEKNEERWFELSSKIE"
     gene            8503..9282
                     /locus_tag="PK218_06415"
     CDS             8503..9282
                     /locus_tag="PK218_06415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW98175.1"
                     /translation="MKHLIKSYLNFLWNSTNQHGVHSPFVFNLVTKCFYDKKQYPEYS
                     IIKKYRNSLLENKKFIEVTDFGAGSRVFKSNKRQISKIANTAGISPKRAELLFRITNY
                     FQPESVLEIGTSLGLATSALSLGNESAKITTLEGCPKTQEIAKFQLENSKFQNIKFVT
                     TKFEEYLSIYNLQPTTYNLIYFDGNHSKKATLEYFELLLQTIANETVWIFDDIHWSKE
                     MEEAWEIIKNHPKVTVTIDTFQWGIVFFRSEQVKENFIIRI"
     gene            9363..10061
                     /locus_tag="PK218_06420"
     CDS             9363..10061
                     /locus_tag="PK218_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW98176.1"
                     /translation="MANPLIDIKQIKRDFVLGSEIINVLKGIDLQINKGEYVALMGPS
                     GSGKSTLMNLLGCLDTATSGTYILNGNDVSQMHDDQLAEIRNKEIGFVFQTFNLLPRT
                     TALDNVALPMIYAGFSKSERNIRATEVLSQVGLSDRMDHQPNQLSGGQRQRVAVARAL
                     VNNPSIILADEPTGNLDSKTSVEIMSLFNDIHKNGNTVILVTHEEDIAKYAHRIIRLR
                     DGMIESDQQNTEHL"
     gene            10255..10629
                     /locus_tag="PK218_06425"
     CDS             10255..10629
                     /locus_tag="PK218_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HPW98177.1"
                     /translation="MKKLFLILIILINTNVFSQSQNVDFDNFILNFNSSKFETIYQTF
                     SPAMKKVRTKEYYSSFLTKIKKDYGTIINYNLLDFKINTQNTIRYNYDTTFENGNLTI
                     RITINNHHKITGLYFLKNNVFL"
     gene            10650..11219
                     /locus_tag="PK218_06430"
     CDS             10650..11219
                     /locus_tag="PK218_06430"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPW98178.1"
                     /translation="MKVYTKTGDKGTTALFGGTRVPKHHIRIESYGTVDELNSHIGLI
                     RDQEINSLYKKVLIEVQDRLFTVGAILATPPEKETLKNGQPRLQNLGIIESDIEFLEK
                     EIDRMEESLPPMTHFVLPGGHTTVSYCHIARCVCRRAERLAVHLNEIEPTDAYVIKYL
                     NRLSDYLFVLARKLSYDLNADEVQWIPRK"
     gene            11394..11615
                     /locus_tag="PK218_06435"
     CDS             11394..11615
                     /locus_tag="PK218_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HPW98179.1"
                     /translation="MYWTLELASYLSDAPWPATKDELIDYAIRAGAPLEVVENLQSIE
                     DEGEIYESMEEIWPDYPTDEDYLWNEDEY"
     gene            11702..15049
                     /gene="secA"
                     /locus_tag="PK218_06440"
     CDS             11702..15049
                     /gene="secA"
                     /locus_tag="PK218_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPW98180.1"
                     /translation="MSFIDSILKAFVGDKSQKDVKSIQPLVNKIKSFENSLMALSNDE
                     LRSKTVEFKQKIKDARAEKDAKIAALKLEAEGIVDIDKKEDIYNEVDVLEKEAYDISE
                     KILLEILPEAFGVVKETARRFKDNTTVEVTTTPKDRELSATKPYITLEGEKTIWSNSW
                     NAAGKEITWDMIHYDVQLIGGIVLHQGKIAEMQTGEGKTLVATLPLYLNALTGNGVHL
                     VTVNDYLARRDSTWKAPLFEFHGMTVDCIDNHSPNSDARRKAYEADITYGTNNEFGFD
                     YLRDNMAHAPEDLVQRKHNYAIVDEVDSVLIDDARTPLIISGPVPDGDRHEFTELKPK
                     IENLYALQRQIAGGFLTEAKRLIKEGNTKDGGFQLLRAYRALPKNKALIKFLSEEGNK
                     ALLQKTENEYMQDNNRKMPIVDEALYFVIEEKNNQVELTDMGIKFLSKDTSEDFFILP
                     DIGTEIAKIEKLNLEKDKEAEAKEKLFQDFGIKSERIHTLTQLLKAYTLFEKDVEYVI
                     MDNKILIVDEQTGRIMDGRRYSDGLHQAIEAKEQVTIEAATQTFATITLQNYFRMYNK
                     LAGMTGTAVTEAGEFWEIYKLDVVEIPTNRPIARNDKEDFIYRSVREKFNAVIEDVTQ
                     LSQSGRPVLIGTTSVEISELLSRMLKMRGINHNVLNAKMHKSEAEIVAEAGKPGVVTI
                     ATNMAGRGTDIKLSPEVKAAGGLAIVGTERHDSRRVDRQLRGRAGRQGDPGSSQFYVS
                     LEDNLMRLFGSERVAKIMDRMGLKEGEVIQHSMMTKSIERAQKKVEENNFGTRKRLLE
                     YDDVMNAQREVVYKRRRHALHGERLKLDIANMMYDTCELIVSQTKGSNDFKNFEFELI
                     RYFSITSPISTSEFDKMSEMEITGKVYKAAMAYYTEKTERSAREAFPIIANVYQNEGN
                     KFERIVVPFSDGIKSLNVVTDLKKAFDTEGRHLVADFEKNITLAIVDEAWKKHLRKMD
                     ELKQSVQLAVHEQKDPLLIYKLEAFNLFRAMLDGVNKEVISFLFKGDLPQQNNTIQEA
                     RQVKRKENYTETKEEINSSESANREAGQAASQRPQVTETIVRETPKINRNDTVTIKHV
                     MSGKTESMKYKKAESMIASGEWVLVSE"
BASE COUNT         5440 a         2416 c         2588 g         4747 t
ORIGIN      
        1 taatagttta catcatcagt aagttttcag gtaaaaaata attgacaaac aatcttaatt
       61 tttataaagt tgcagtacgt cgattttatt ttcatttgca cttaataatt tttttgtttt
      121 gctagctatg tttagtttta aacaaaatta atattgcaat ctactaccaa cttatgaaaa
      181 aactttactc attgcttctt ttatttttca tttcaggaaa tgccacaaat tattatgtga
      241 gtcaattaac tggaattatg tcaaatgatg gtctaacccc tgaaaccgct tttttaactc
      301 ttcagcaagc ccatgacatt tctcagcctg gtgatgtcat ttttatcatg aacggtatat
      361 attcaaatga caatgctgat agtgacatct tagaaattgt aatatctgga actgaaaatc
      421 aatggataac ttacaaaaat tatgaagggc atcaacctgt tttaaaatta aatgctaata
      481 attggcaagc ggttaacatc caaggagcag attatattat tattgatgga ttgaaaatta
      541 ttggaaataa tgatgctatt tctctcgaat atgcactttc tgaacaaaat aattttgaca
      601 atgctgccac ttctggaaat ggaattgcta tttcaattga atttgataat caagaaaata
      661 aaagtcatca tgtcattatt agaaattgta cgatttcaaa atgtggcggt ggcggaattt
      721 acagctatca ctcggattat ataactattg aaaacaacac agtttatgaa tgtgcttggt
      781 attcgccata caataatagt ggaatttcta tgtatcaaaa ctggaattca gataattcat
      841 tggaaattaa aaacatcatt tcgggcaata cttgttatag aaacgaatgt tatatcccaa
      901 gattaattga aggtgttcca acgataattg atggaaatgg tattgtaata gatgatagta
      961 gaaatacgca atttgattcg ccattaggtg cttatttagg taaaacctat atcataaaca
     1021 atgtgatttt tgataatggt gctagaggaa ttcatagtta ttcttcggat aatatcatcg
     1081 tttctaataa tacattgtat aaaaactgtc agtctgatga tactcaagaa ggtgaattaa
     1141 ccgctttcga ttcaagtgct attacctaca tcaataacat tgttttccct gatgtaaata
     1201 ttccgcctat tgcaatttac aatattgctg atttaattgt agattataat ctttgggcag
     1261 aaaactcaga tttggctgca cattttggga caaacacatt aattggaaat ccagattttg
     1321 tgtttccgag tactaatcct ttagaagcag attttcattt gatgccaagt agtatcgcta
     1381 taaatagtgg ttcttcaact gttgcaccaa taactgataa agacggtaac tccagaattg
     1441 ataatgtgga tttgggtgct tatgagtttc aacccaacct aaatactata aagattcaag
     1501 atgcttcctt cattatttat ccaaatccag taaaagaaag tttaacaatt gttttttcag
     1561 aaaacaattc ggaattggtt gaaatcagta tttacaattc actaggacaa agtattgctt
     1621 cttccctaaa agcaatagct aaccattcgt taaccatccc tttaaaccac ttatctaatg
     1681 ggatttacta tgtaactata agcaaaaatg gcaaacaaat tgacactaaa aaaatcatta
     1741 aaatataagt ttcaatttat ttaaaatatt taacaactaa tcgctttttg attttgtgta
     1801 agcaaaaaat aaggttaatt ttgcatatta attttaacac cttaaaatgt ctaacacaca
     1861 acacttacac gattttacaa cacaagttcg aagagacatt cttcgtatgg ttcatgctgt
     1921 aaattcaggt catcctggtg gttcactagg ctgtgctgaa ttttttactg ttttatatca
     1981 aaacatttta gaaagaaaac ctggttttga catggacggc attggcgaag atttattctt
     2041 cttatctaat ggtcatattt ctcctgtttt ctacagcgtt ttagctagaa gcggttattt
     2101 tccagtttct gaattggcta cttttagaaa attaaatact cgtttacaag gacacccaac
     2161 tactcatgaa ggtttgcctg gagttcgagt ggcatcgggt tctttaggac aaggtttatc
     2221 ggttgccatt ggagcagctc aagctaaaaa attaaataaa gactcacatt tagtatatgc
     2281 acttactggt gatggagaat tacaagaagg tcaaaactgg gaagctataa tgtatgcttc
     2341 tgccaaaaaa gtggataatt taattgcaac tgttgacttg aatggtcagc aaattgatgg
     2401 ttctacagat gaagttttaa atatgggaag cataaaagcc aagtttgaag cttttgattg
     2461 ggatgtttta gaaatcaaag aaggaaacaa cattgaagca gttttaactg gaatgaatga
     2521 agcaaaaagc agaactggaa aaggaaaacc aatttgtgtt ttattgcata ccgttatggg
     2581 caatggtgta gattttatga tgcacacaca tgcttggcac ggaaaagcac caaacaacga
     2641 acaattagca acaggattag cacaaaatgc agaaacttta ggagattatt aaatagaatt
     2701 aagatttgag acttcagaag taagacttaa attttaaagt aattatatta tgaaaaaata
     2761 cgaaaatcaa ggaagtaaag atacccgttc tggatttggt gcaggaatga ccgaattagg
     2821 acaaaaaaac gaaaatgtgg tagcactttg tgccgattta ataggttcgt taaagtttga
     2881 cgatttcaaa aagaatcacc cagaacgttt ttttcaaatt ggaattgcag aagccaatat
     2941 gattggaatt gcagctggtt taactattgg tggaaaaatt ccattcacag gaacatttgc
     3001 taacttttct acaggaagag tttatgacca aatccgtcaa tcggtggcat attcggataa
     3061 gaatgtaaaa atttgcgctt cgcatgctgg attaacactt ggagaagatg gtgcaacaca
     3121 ccaaatctta gaagatattg ggttgatgaa aatgttaccc ggaatgacgg ttatcaatac
     3181 ttgtgattac aatcaaacca aagcggcaac tattgctatt gctgatcatc atggtccagt
     3241 ttacttgcgt tttggtcgtc cagttgtgcc taacttcact cctactaacc aaacatttga
     3301 aattggaaaa gcaattctat taaatgaagg aacagatgta acaattgttg caactggcca
     3361 tttggtttgg gaagcattag tagctgctga agccttagaa gccaaaggaa tttcggcaga
     3421 agtaattaat attcacacta taaaaccttt ggatgatgca gccattttaa aatcggttgc
     3481 aaaaactggt tgtgttgtta ctgctgaaga acataatttt cttggtggat tgggtgaaag
     3541 tgtttcaaga gttttagcat taaataaccc aaaaccacaa gagtttgttg ctgttaatga
     3601 tagttttgga gaaagtggaa cacctgagca actgatggaa aaatacaagt taaacaatca
     3661 agcaattgtt gaagcagtag aaaaagtatt gaagagaaaa taatttattt ttagaacttc
     3721 tacaaagcaa aattcccgat ttttcaatcg ggaatttttt atttctaata atctctattt
     3781 tcatcaaaag cttcaagata ttcggctact cgttttacaa aacttccacc taatgcacca
     3841 tctacaactc ggtggtcata actgtgtgac aagaacattt tttgacgaat tccaataaaa
     3901 tcaccatctg gagtttcgat aactgctggc acttttctaa tggctcctag cgctaaaatt
     3961 ccaacttgtg gttggttgat tattggtgtt ccaaatacag aaccaaaagt cccaacattg
     4021 gttacggtat atgttccgcc ttgtgtatcg tctggtttta gttttccggc tttggctcta
     4081 tttcctaaat catttactgc ttttgccatt ccaacgatat ttaattcatc tgcattttta
     4141 ataacaggaa caattaaatt tccatttggt aaagcagctg ccattcctaa attgatattt
     4201 tttctcttga tgattttatc accatctaca gaaatattca tcattggaaa atctttcaat
     4261 gctctggcaa cggcttccat aaaaattggt gtaaaggtca atttttcgcc ttctcgtttt
     4321 tcaaatgctg tttttacttt atcgcgccat tttacaatat tagttacatc aacttcaata
     4381 aatgattgaa cgtgtgccga tgtttgcact gaagccacca tataaccaga aatcaactta
     4441 cgcattctgt ccatttcgat gatttcgtcg ccaccattaa cagaaaccgg agctttaact
     4501 gattccgttt tagaaattgt agccgctggt tgtggagaag cttttggtgt ttcttctact
     4561 ttttgttcta caatttgtgg cgttgcaact ggaacaggag cttcaatcgt tgctactttt
     4621 gctacaccgt ttgtatttct tttggctaca aaagttaaaa tatcttcttt tgtaactctt
     4681 ccttcttttc ctgaacccga aatagaattt aattcaccaa cagaaacacc ttcttgaagt
     4741 gctatattct taactaatgg tgagaaaaat ttatcagaat tagaataatc tgctttttct
     4801 aaaacaactg gcgcaacact ttctcttgct gcttcaaaag ttttagtaac ttctgcaacg
     4861 gcaactggag gatttacttg tacttcagga gttgaaacag cactatttac ttctgtttct
     4921 atgtatgcaa tggtttgacc aacttgaaca acatcattta cttgaaataa tatttcggtt
     4981 aatgttccag agacttcact tggtacttcg ctatctactt tgtcagtagc gatttcaagt
     5041 acagcttcat ctgcattaac tgtatctcca acgtttttta accaattggt aatggttgct
     5101 tctgcaacac tttctcccat ttttggtaat ttcaattcaa actttgccat aattgtaaac
     5161 taagtgtggt tgtatttcgt tgcaaaatta ctaattattg cgcttatgct acgaattgct
     5221 ttttttattt attaataatt tcgaagctac ttttcaacaa ttgaaattaa ataaaaataa
     5281 tttctcctct gtcattacta ctgttgctgc ctattaaata gccattttct ttaattaaaa
     5341 atttgaaagt aaatctttta gcattttgtt ccataaaaat caacgcttct ttgaaactgc
     5401 aatacgaaca gtcaaatatt atctctgttt tctcgtgctc cgaaatcgtt tgcgaaaata
     5461 ctacttttcc attttctatc gttgcaagtt gaatgttttt ttgaagttct tttgacaatt
     5521 tatctttaag ttctaatgtg ctagaaatta aaacataact atctggtgaa gattttgctg
     5581 gcttttcagc ttgatatttt tttacaaatg aaaaaaatat agaacccatt ttcatcataa
     5641 agctaaagaa atatgatttc ttaaaatgtt ttctataaaa gtaattcata gcttcttgaa
     5701 aacgcttcat gtacttttca tctctaactg tactttctcc tttaaaatga attacagttg
     5761 tatcatgata ataataattt gcttttccgg tttgtaaaac catataactc aaatcaatat
     5821 cgtcagaata cataaagcaa ttttcatcaa aaccgccaac ttcattataa agttcgcgtt
     5881 tcattatcat gaatgcacca acaagaattt ctacatttcc agtttgattt ttatctaaat
     5941 gttgtgcata atatttgtta aacaaactgc ttttcggaaa ccatttatac aaaccaaaaa
     6001 cttttgtaaa agcaacccaa ggcgttggaa taccacgctt actttcgggt aaaaattgtc
     6061 ccgaaccatc aattaattta caaccgatta ttccagcgga aagttgcgag tttggcactt
     6121 cgactgcgct cagtgtgaca aaatttagaa ttttaacaaa tgtatcttct gcaactattg
     6181 tatccggatt taaaatacaa ataaattcgc ctttagcttc atttactgca atgttatttc
     6241 cttttggaaa accataattc tctttgtttt caatgagttt tatgtttgga aatttagttt
     6301 tcatcatcgc acaactatca tctgaagaat gattatcaat aacaatgatt tcagcatcta
     6361 tattttgaat ggctttttgt acactcaaaa cacatacttc taaaaagtag cgaacgttat
     6421 aattaaggat aattacggat aattgcattt gacaaatata gggtaatttg aaaatgttac
     6481 aatttgaaaa tttgaaaatg ctcttaataa ataatgtact acttttgcaa accagttaat
     6541 tttaaaatta acaattatca aatcttcaaa ttaaaatgaa ttacctttca gtagaaaata
     6601 tatccaaagc ttttggcgaa cgtgtattgt ttaaagacat ctcttttggc atcaataaag
     6661 accaaaaaat tgcatttatt gccaaaaatg gttcgggaaa aacctgtatc atgaagatta
     6721 ttaacggcga agatgaagca gatagcggac aagtagtagt ccgaaaagga ttgaacatgg
     6781 cgtttttgtc gcaaagcaat aatttgcaag acgaattgac gattgaagaa agtatttttg
     6841 cttcggacaa cgaaatacta aaagtaattg aacgatacga aaaagcatta gaaaatccag
     6901 aagatgcaga taaatatcag ttggcatttg atgaaatgga cagttataat gcatgggatt
     6961 ttgaaacaca gttcaaacaa attctttcca aattaaaact ggatgactta aaacttaaag
     7021 tgaaatcgct ttctggtgga caaaaaaaac gtttatcgtt agcgataatt ttgattaatc
     7081 gacctgattt gttgattttg gatgagccaa ccaatcattt ggatttggaa atgatagaat
     7141 ggctggaaag ttattttgca aaagaaaaca ttacgctgtt tatggtgacg cacgaccgtt
     7201 ttttcttaga acgtgtttgt aacgaaatca tcgaattaga caacggaaaa ttatattctt
     7261 ataaaggaaa ttactcctat tatttagaga aaaaagaaga acgtattgct atggaaaatt
     7321 cttctataga caaagcgcaa aatctttttg tgaaagaatt ggcttggatg agacgtcaac
     7381 cgaaagccag aacgaccaaa tcaaaatcac gtcaagatga tttttatgtg attaaagaaa
     7441 aagccgaaag tcgcagaaaa gaaaacgtag tagaactcga aatcaacatg gaacgcatgg
     7501 gaagcaagat tatcgagttg cacaaattgt tcaaaaaatt taaagaaaaa acaattttag
     7561 acaactttag ctacgatttt caacgaggcg aacggattgg aattattggt aaaaatggaa
     7621 caggaaaatc tactttcttg aatattttga ccaaaactat gctgccagac gctggaaaag
     7681 tagttgttgg tgacacgata aaaattggct attatacaca aagcggtatt aacccaaaac
     7741 caggacaaaa agttattgat ataatcaaag aatatggcga atacattcca ttaacgaaag
     7801 gaaaaataat ttctgcttca caattattgg aacgcttcct ttttgatgcc aaaaaacaat
     7861 atgattttgt agaaaaattg agtggtggcg aattaaaacg cttgtaccta tgtacggttt
     7921 taattcaaaa tcctaacttt ttaattctcg atgaaccaac gaatgattta gacattgtta
     7981 ccttgaatgt tttggaaagt ttcttgttag attatccagg ttgtttgctt gtggtttcgc
     8041 acgatcgcta tttcatggat aaaattgtgg atcatttatt cattttccgt ggcgaaggtc
     8101 agattgagga tttcccagga aattattctg attttagatc gtatgaagac agtgtagaac
     8161 caaaaaactt atctaacgta agcaccgaaa agactaattg gaaacaaaat aacccaacta
     8221 attcgggctt aaatttcaat gaacaaaagg aattcaacaa aatcgaaaga gaaatcaaag
     8281 atttagaata cgagaaaaaa caaattgaaa acttattttc agaaggaaaa gttgctgatg
     8341 accaaattga gaaaaaagcc atcgaacttc agaaagtaat tgaaagattg gaaaaaaacg
     8401 aagagcgttg gtttgaactt tcttctaaaa ttgaataaat tacaaacaca aaattcacag
     8461 atttcacaaa ttaatctgtg aaatttgtga aatttgtgtc aaatgaaaca tctaattaaa
     8521 tcatatttaa attttctgtg gaattcaaca aaccaacatg gagtgcattc tccatttgta
     8581 tttaatttag tgaccaaatg tttctatgac aagaaacaat atcctgaata ttcaattata
     8641 aaaaaatata gaaattcact tctagaaaac aaaaaattca ttgaagtaac tgattttggt
     8701 gcaggttcaa gagtttttaa atcaaataaa agacaaatct caaaaattgc caacactgct
     8761 ggaatttctc caaaaagagc cgaattactt ttcagaatta caaattattt tcaacctgaa
     8821 agtgttttag aaattggaac ttcgctagga ttagcaactt ctgccctatc tttaggaaat
     8881 gaatctgcta aaataactac attagaaggt tgtccaaaaa ctcaagaaat tgcaaaattc
     8941 caattagaaa attccaaatt ccaaaatata aaattcgtaa ccacaaaatt tgaagaatac
     9001 ctttcaatct acaacctaca acctacaacc tacaacctaa tctacttcga cggcaaccat
     9061 tccaaaaaag cgacactaga atattttgag cttttattac aaacaattgc aaatgaaaca
     9121 gtttggattt tcgatgacat acattggtca aaagaaatgg aagaagcttg ggaaatcatt
     9181 aaaaatcatc caaaagtaac agtaactatc gatacttttc agtggggaat tgtctttttt
     9241 agaagcgaac aggtgaaaga aaattttatt attagaattt aacttgtaac aaattagccg
     9301 ttttgaatac ctatatttac attcaataat tccaaaatcg ttaatcgtca atcataactc
     9361 aaatggcaaa tccgttaata gacatcaaac aaattaaaag agattttgtt ctaggtagcg
     9421 aaattataaa tgttttaaaa ggcattgatt tacaaatcaa taaaggcgaa tatgtcgcat
     9481 tgatggggcc aagtggttca ggaaaatcaa ccctaatgaa tcttttagga tgtttggata
     9541 ctgcaacttc tggaacttac attcttaatg gaaatgatgt ctcacaaatg catgatgatc
     9601 aattagctga gataagaaac aaagaaattg gatttgtttt tcaaacattt aaccttttac
     9661 caagaacaac tgctttagac aatgttgctt taccaatgat ctatgcagga ttttcaaaat
     9721 ccgaacggaa tatacgtgct acagaagttt taagtcaagt tggattaagt gacagaatgg
     9781 atcatcaacc caaccaactt tctggtggtc aacgccaacg tgtagcagta gcaagagcat
     9841 tagtcaataa tccttcaatc attcttgccg acgaaccaac tggaaattta gatagtaaaa
     9901 cctcggtaga aattatgagt ctttttaatg atattcacaa aaatgggaat accgtaatcc
     9961 tagtaaccca cgaagaagac atagccaaat atgcacaccg aatcatccgt ttgcgtgatg
    10021 gaatgattga aagtgaccaa caaaacacag agcatcttta ataattgaag cataagtttt
    10081 gggcattttt agaaatgtaa ttcatgcttt ttgcagtatc ttttttgtca tgctattcgc
    10141 agtctagacg caattttctc aaaaaaggat acttgcttca atcagggcta gatgaataaa
    10201 ccatttgaat ttttagtaac tttgggactt taaaattgtc aattctatta tctattgaaa
    10261 aagctatttc taatattaat tatcctcatt aacacaaacg tcttttcaca aagtcaaaat
    10321 gttgatttcg ataattttat tttaaatttc aattcttcaa aatttgaaac catctaccaa
    10381 actttttcgc ctgccatgaa aaaagtaagg acaaaagaat attatagttc ttttttaacc
    10441 aaaataaaaa aagactacgg aacaataata aattataatt tgttagattt caaaataaat
    10501 acacaaaaca caatccgcta caattatgac actacttttg aaaacgggaa cctaacaata
    10561 agaataacca ttaacaatca tcataaaatt acaggtttat atttcttaaa aaacaatgta
    10621 tttctataaa tctaaagtct aaaatccata tgaaagtata tacaaaaaca ggcgacaaag
    10681 gaacaacagc tctttttggc ggaacaagag ttccaaagca ccacatccga attgaaagtt
    10741 atggaacagt tgacgaactc aactcacata ttggtttaat tcgagaccaa gaaatcaatt
    10801 cattatataa aaaagttcta atagaagttc aagaccgatt gtttactgta ggcgcaatac
    10861 ttgccacacc acccgaaaaa gaaacactaa aaaatggtca accacgactt caaaatttag
    10921 gaattataga atccgatatt gagtttttag aaaaagaaat tgataggatg gaagaaagtt
    10981 tgccaccgat gacacatttt gttttgccag gcggacatac tactgtgtca tattgtcata
    11041 ttgctcgttg cgtttgccga agagctgagc gtttagcagt acatttaaac gaaattgaac
    11101 ccacagatgc ctatgttatc aaatacctaa accgactttc tgactatctt tttgtattgg
    11161 cacggaagtt gtcttacgat ttgaacgcag atgaagtaca atggattcca cgtaaataaa
    11221 attcaagaaa ataaaaccca aactccaacg gtaaaaacga aaacaatgga atttggaatt
    11281 taaaatttga atttaaaaaa agccgttctt atcttttttt taaaataaat cattttttac
    11341 ttgacttttt cagtaataaa tttatttttg cataaaatta aacccgatag acaatgtact
    11401 ggacattaga attagcatcc tatttaagcg atgcaccgtg gcctgcgact aaagacgaat
    11461 taatcgacta cgctattaga gctggtgcgc cacttgaagt agtagaaaat cttcaatcaa
    11521 ttgaagacga aggcgaaata tatgaatcga tggaagaaat ttggccagat tatccaactg
    11581 acgaagacta tctttggaac gaggatgaat attaaaaaaa taagtaaaca ctaagaaaga
    11641 aaagtccttt gagggctttt tttttgttta aatttgcaca ctttaaaaac ataacaataa
    11701 tatgagtttc attgatagta ttcttaaagc ttttgttgga gataaatctc aaaaagatgt
    11761 taaatcaata caacctttag taaataaaat taaatcattc gaaaatagct taatggcatt
    11821 atctaatgat gaattgcgta gcaaaactgt tgaattcaaa cagaaaatca aagatgccag
    11881 agccgaaaaa gatgctaaaa ttgcagcttt aaaattggaa gcagaaggca tcgttgatat
    11941 tgataaaaaa gaagatattt ataatgaagt ggatgtttta gaaaaagaag cttacgatat
    12001 ttcagaaaaa atattattag aaattcttcc agaagctttt ggtgttgtaa aagaaaccgc
    12061 acgccgtttt aaagacaaca ctactgttga agtaacaaca actccaaaag acagagaact
    12121 ttctgctaca aaaccttata ttacattaga aggagaaaag accatttggt ctaattcatg
    12181 gaatgcagcg ggtaaagaaa ttacttggga catgatccac tacgacgttc agttgattgg
    12241 aggaattgtt ctacaccaag gaaagattgc tgaaatgcaa acaggtgaag gtaaaacttt
    12301 agtagcaaca cttccactct atcttaatgc cttaaccgga aatggtgttc atcttgtaac
    12361 cgtgaatgac tatttggcaa gacgtgacag tacatggaaa gcaccattat ttgaattcca
    12421 cggaatgact gtggattgta ttgacaatca ttcaccaaac tcggatgcaa gaagaaaagc
    12481 atacgaagca gatattactt atggaacaaa taatgaattt ggttttgatt atttgcgtga
    12541 taatatggcg catgctccag aagatttggt gcaaagaaaa cacaactacg ccattgtgga
    12601 tgaggtggat tctgtattaa ttgacgatgc aagaacgcca ttaattattt cgggtccagt
    12661 tcctgatggt gatagacatg agtttactga attaaaacca aaaattgaaa atctttatgc
    12721 acttcaacga caaattgctg gtggcttttt gaccgaagca aaacgtttaa tcaaagaagg
    12781 aaataccaaa gacggtggtt tccaattgtt gagagcgtat agagctttac caaaaaacaa
    12841 agcattaatc aagtttttga gtgaagaagg gaataaagct ttgttgcaaa aaacagaaaa
    12901 tgaatacatg caagataaca accgaaaaat gccaatcgtt gatgaagcat tatattttgt
    12961 tattgaagaa aaaaacaatc aggtagaatt gacggatatg ggaatcaaat tcttatccaa
    13021 agacacatca gaagatttct ttattcttcc agacattgga actgaaattg caaaaattga
    13081 aaaacttaat ttagaaaaag ataaagaagc cgaagctaaa gaaaaattat ttcaagactt
    13141 tggtataaaa tcagaacgta ttcacacttt aacgcaactt ttaaaagcct acactttatt
    13201 tgaaaaagat gtagaatatg tgataatgga caacaaaatc cttattgttg acgagcaaac
    13261 tggtcgtatc atggatggaa gacgttattc tgacggtttg catcaagcta ttgaagccaa
    13321 agaacaagtt accattgagg ctgctacaca aacttttgca accattactc ttcagaatta
    13381 tttccgtatg tacaataaat tagcaggaat gactggaact gctgttacag aagcgggcga
    13441 attttgggaa atttataaat tagatgtagt tgaaattcca accaatagac ctattgctag
    13501 aaatgataaa gaagatttca tttaccgttc agttcgtgaa aaattcaatg ccgtaatcga
    13561 agatgttaca caactatcac aatctggaag acccgtttta attggaacaa cttcggttga
    13621 aatctccgaa ttattgagta gaatgttgaa aatgcgtggt attaatcata acgtattgaa
    13681 tgccaaaatg cacaaaagtg aagcagaaat tgttgccgaa gctggaaaac caggcgtagt
    13741 aactattgca accaatatgg ccggtcgtgg aacagatatt aaattgtctc cagaagtaaa
    13801 agccgctggt ggattagcta tcgttggtac agaacgtcac gattcacgac gtgttgaccg
    13861 ccagttacgt ggtcgtgctg gtcgtcaagg tgatccggga agttcgcagt tttatgtttc
    13921 tttagaggat aatttgatgc gtttatttgg ttccgaaaga gtagccaaaa ttatggatag
    13981 aatgggcttg aaagaaggcg aagtgattca gcattcgatg atgaccaaat ctattgaacg
    14041 tgctcagaaa aaagtggaag aaaacaactt cggtacacgt aagcgtttgc tagaatatga
    14101 tgatgttatg aatgcgcaac gtgaagtagt ttacaaacgt cgtcgtcacg ccttgcatgg
    14161 agaacgttta aaattagaca tcgccaacat gatgtacgac acttgcgagt tgattgtaag
    14221 ccaaaccaaa ggatcgaatg atttcaaaaa ctttgaattc gaattaattc gttatttctc
    14281 aattacatct ccaatttcga cttcagaatt tgataaaatg tctgaaatgg aaatcacggg
    14341 taaagtatat aaagcggcaa tggcttatta tacagaaaaa acagaaagaa gtgcacgaga
    14401 agctttccca ataattgcta atgtttatca aaatgaaggc aacaaatttg aacgtatcgt
    14461 agttcctttt tcagatggaa ttaaaagttt gaatgtagtt accgatttga aaaaagcatt
    14521 cgatacagaa ggaagacatc ttgtggctga ttttgagaaa aacatcacac ttgctattgt
    14581 tgatgaagct tggaaaaagc atttacgtaa aatggatgag ttgaaacagt cggttcaatt
    14641 ggctgttcat gagcaaaaag atcctttact aatttataaa ttggaagcct tcaacttatt
    14701 tagagcaatg cttgatggcg ttaataaaga agtgatttcg ttcttgttta aaggtgattt
    14761 gccacagcaa aacaatacta ttcaagaagc acgtcaagta aagcgcaaag agaattacac
    14821 cgaaacaaaa gaagaaatta atagtagcga aagtgcaaat agagaagctg gtcaagcagc
    14881 atcacaacgt cctcaggtta ctgaaactat cgttcgtgaa actccaaaaa taaatagaaa
    14941 cgacaccgtt acaattaaac atgtaatgag tggaaaaaca gaatccatga aatacaaaaa
    15001 agcagaaagt atgattgctt ctggcgaatg ggttttagtt tctgaataat atttaattaa
    15061 ctataatttc aatcctcaat agtaaaacca ttgaggattt tttaattaaa atatgttgaa
    15121 gcaacattta taaaaagatt ttttttaaat ttgaaaaaaa agatatgaaa aaaaatacgc
    15181 tattatatgt c
//
LOCUS       DAOOBH010000048        14999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5156_length_14999_cov_5.668563, whole genome shotgun sequence.
ACCESSION   DAOOBH010000048 DAOOBH010000000
VERSION     DAOOBH010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14999
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5156_length_14999_cov_5.668563"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..988
                     /locus_tag="PK218_06445"
     CDS             5..988
                     /locus_tag="PK218_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPW98181.1"
                     /translation="MLQVKRLLLIGFLMNTTYHFAQYTDIINSNRPGKSMSAYSVGKT
                     VIQAEGGFFGIKEKHDLARYEANGFGSDLSVRYGAFFEQFELMLDMQYQKDWYTAPLV
                     DKTRSGLRQTTIGAKYLVYDPFKNLREDKPNLYSWKANHSNKFDWKQLIPAVALYAGV
                     NLRIGNNPFYVDDAFISPKGMIITQNHFGTHWVFVNNFILDKVTTKDANIGLVSSLTR
                     GFNMRWSGLLEFQATKSDYYADYLFRVGAAYLIRENIQIDASISQNVKDTPYILAGGF
                     GLSWRFDANYETVYLRSQKPEDPKEKAKDKKAKKDKKKKRKDEVELEETKP"
     gene            989..2107
                     /locus_tag="PK218_06450"
     CDS             989..2107
                     /locus_tag="PK218_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase"
                     /protein_id="HPW98182.1"
                     /translation="MITIVEANSPKLLKDFVKFPFDLYKNHPYWVPPLISEELETFDK
                     TKNPAFTTAEATFYLAYRENKIVGRIAAIINWNEVNDQNKKKVRFGWWETIDDVEVTK
                     ALLEKVKELGEQNQLEHIEGPMGFSNLDKVGVVTEGFDEIGSMITWYNYPYYKEHLEL
                     LGFVKEKEYHENKFPFSNVKPEFFFRVQEMIKKRYQLTPINFTKTKDIMPYVDEMFDL
                     FNKSYASLASFVAITDIQKEYFKKKYISFINPEYIKFIVDKDHKIIAFSIVMPSFSEA
                     LQKANGRLFPLGFYHLLKAKKNSKEVLFYLIGVDPEYQNKGVTAVIFNEYYECFKTKG
                     IESCVRTPELADNYAIQNIWKHFDPVVFRKRCTYIKNL"
     gene            complement(2113..3063)
                     /locus_tag="PK218_06455"
     CDS             complement(2113..3063)
                     /locus_tag="PK218_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPW98183.1"
                     /translation="MIVWILFLAAILFFLALDLGVFNKTPHIISSKEAGKWTAIFVTI
                     SFLFSGVIYWVYQNGFIENPTKLSPSLASIKYITGYLIELSLSVDNIFVIAVIFASFK
                     IPQKYQHRVLFWGILGAIAFRGLMIYFGVLLINKFSWMTYVFGAFLIFTAAKMLFKGE
                     EEEFNPKKSFIFRSMRKIIPVTMHMDGQKFFIKKRQITFATPLFVALIVIEVMDVLFA
                     MDSVPAILAITSDPFLVFSSNIFAILGLRSMYFFLANMLEKFSYLEYSLIAILTFVGI
                     KMLAIHYFKFPEWVSLGFIALSLAIGIIFSLYKDKQSIKV"
     gene            3246..5435
                     /locus_tag="PK218_06460"
     CDS             3246..5435
                     /locus_tag="PK218_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPW98184.1"
                     /translation="MATLRFQALHDASSRKPVKFEESGKKSELFGSNVFNDKAMRQFL
                     TSDAYKGVKDAILHGSKIDRKLADYIAMGMKEWAITKGVTHYTHWFQPLTGATAEKHD
                     AFFETSYDGSDPVEKFGGGQLVQQEPDASSFPNGGIRNTFEARGYTAWDPTSPAFIFG
                     TTLCIPTVFISYTGEALDYKTPLLRALNAVDEAATDVCKYFDKNVKKVTATLGWEQEY
                     FLVDSALANTRPDLILTGRTLLGHTSSKGQQLDDHYFGSIPSRALSYMRELEEECMLL
                     GIPVKTRHNEVAPNQFELAPIFEETNLAVDHNSLLMDVMSKVAERHDFKVLFHEKPFK
                     GVNGSGKHNNWSLATDTGVNLLSPSKTPMSNLQFLAFFINTIKAVQEYEELLRAAIAT
                     ASNDHRLGANEAPPAIISVFIGEQLTKVLAELEGVSKGKLSPEEKTDLKLNVVGKIPD
                     VMLDNTDRNRTSPFAFTGNKFEFRAVGSTANCANAMTTLNSIVAKQLKDFKKEVDALI
                     EKKDLKKDEAIFNVLREYIKETKNILFEGDGYSDAWEKEAKKRGLSNHKTTPAALKAK
                     VSKKALDLFAGLQVMNHVEVEARYEIELEEYTKKIQIEGRVLGDIARNHIIPTAIRYQ
                     NTLIENVRGLKEIFGKEFEKIAKEQIVLIKEISAHIEGINTNVDAMIEARKKANALTD
                     AQKMAEMYCDKVKPYFEVIREHCDKLELLVDDEVWTMTKYRELLFTR"
     gene            complement(5497..6618)
                     /locus_tag="PK218_06465"
     CDS             complement(5497..6618)
                     /locus_tag="PK218_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98185.1"
                     /translation="MKKIITLSLLLVTLFSFGQVPQGISYQAIAMNTAGNPVVSSNVR
                     VRLSILDASVTGTSLYSETHLKTTNTQGLFNLIIGQGTVVSGVFNTINWATNSKFLKV
                     EMDVTGGTTFANVGTTQLLSVPYALAADSLITSPGEGITLVSPNGTPFVVSVNDAGQL
                     SLPTSGSSSPSSPNNFYMYGTFNTFNPTTALLMANYNNGGNLDRYGYKYITAGSDLKF
                     LATNNASSPVYGVDLSNNLQVNGANFLVDTNGFYLIDLNNFNNPSYPVNDFRLSLNSL
                     APQLINSLTGSFTNSTYSVATNKFSFNLSISANTTNRYEFLYLDPKGSTHGDNLADGS
                     IESNGTSIPISNTTSSPKNYRVELTINFNGSASYTVTQL"
     gene            complement(6699..7154)
                     /locus_tag="PK218_06470"
     CDS             complement(6699..7154)
                     /locus_tag="PK218_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98186.1"
                     /translation="MKKNLLFLFLTTTFSVNCQSVIQSVNSGSILAPNSTVSIGEIIV
                     IPENANQSTSGIIGILAQVNQQNLEVPELALNDKIVVYPNPTMNSITFQTENSLENKK
                     VAIVNLSGQIVAKKQISSSNSLDLSELKTGVYLIQFENKNINSFKIIKK"
     gene            7264..8154
                     /locus_tag="PK218_06475"
     CDS             7264..8154
                     /locus_tag="PK218_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW98187.1"
                     /translation="MKYRILFALLFISFWGYSQLQFTVFFETNKYQLNDREDYKLQSW
                     ISENRNNKVVAIHGFTDEDGTTSSNDTLAQKRVKAIFEIINGKMPIREDFKTISFGEN
                     FNQSKNKADNRKVTIYYILEKDLARENEILGIKEAPKLIEVAPKAEINYPDKMVFENP
                     NGTTSEFKLDVEFMKKVGNATVGEKLKIDNLEFMINTFAVVNESRGKLYELLLVLQHN
                     PQLKIEIQGHLCCMPVDRTDLSTKRAKAIYSFLVANQIYPPRLSYKGFGSSQPIFPIP
                     EKNEAERAANRRVEIVIVAN"
     gene            8156..8998
                     /locus_tag="PK218_06480"
     CDS             8156..8998
                     /locus_tag="PK218_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW98188.1"
                     /translation="MKLFLAYLFFGVTTIALGQEKHFEVFFDFNKDVPTENSMVLLNN
                     WMKSSENIEIVAVSGYCDSIDSNAYNKELANKRIKTVLEIIRGNAIPIKQDLSIYVFG
                     EEFKQSKTQAENRKVIIEYFQKLQSKKEDEASNAPMEAVTIDAEDKVETERITLVEKF
                     EKAKVGDRIAIYNINFMFNSENIELKSEPLLEELLFIMERNPKMVIKIHGHICCNPNP
                     YNTKLSYRRALKIFKYLKENGIQQNRLAYNGVGSNNPIYPIPEKNENERIANRRVEIE
                     IVRK"
     gene            9000..9770
                     /locus_tag="PK218_06485"
     CDS             9000..9770
                     /locus_tag="PK218_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW98189.1"
                     /translation="MKTIVFLILGFFTSICFAQKQFEVFFDFNKDFPNQQSILDFNGW
                     KATSNINEVIRIEGYCDSVDTKNYNKKLAERRIESVLKLLKSSSVAITKDIKKIAVGK
                     DFEQSQVQEENRKVIIYYTEPSLEKPKSVLYESIKNAKVGERIKLSNIYFYNNSAQTV
                     SKSQTTLYDLLCVMEDNPKLKIEIQGHICCQIETDINDVSTARAKAIYNFLIRNKIDR
                     TRMSYKGFGTTRPIYPIPEKNEIEANENRRVEIMIVEN"
     gene            9930..11108
                     /locus_tag="PK218_06490"
     CDS             9930..11108
                     /locus_tag="PK218_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPW98190.1"
                     /translation="MSSDNSQRYNLRGVSASKEDVHNAIKNIDKGLFPKAFCKIVPDY
                     LTNDDDYCLIMHADGAGTKSSLAYLYWKETGDISVWKGIAQDALIMNIDDLLCVGATD
                     NIMLSSTIGRNKNLIPSEVLSAIINGTEELIADLKKFGVTIHSTGGETADVGDLVRTI
                     IVDSTVTARMKRSDVIDNANIQAGDVIVGMASFGQANYETQYNGGMGSNGLTSARHDV
                     FAKYLADKYPESYDNSVPTDLVYSGNIKLTDAVENAPLDAGKLVLSPTRTYAPIIKAI
                     LDKYTPNDIHGMVHCSGGAQTKVLHFIDNVHVIKDNLFPIPPLFQLIQAQSKTDWKEM
                     YQVFNCGHRMEVYVPQEIANDIIEISKSFGVDAQIVGSVAANSTKKLTIKSEFGVFEY
                     "
     gene            12227..13867
                     /locus_tag="PK218_06495"
     CDS             12227..13867
                     /locus_tag="PK218_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW98191.1"
                     /translation="MLQIIAIIIWYNETKHTEAYDNLKATNKILSYTDSLNTNLLHSQ
                     VLFTTYRLNNDSDILTNYGVSLEKANRYLDSLKSETKNKNLLKKVVNKKQKSEVEIRN
                     IKKSIDSIIHLQTTFSIENNLKPINLKPFELKNVLDNVKEDSYIKIDSVSKKRLFSRL
                     SDAFAGRVSVQKEYLNTVVTIQYKDKVSKGTFEEQIATAIGYSNNYYSKQISQLQQSF
                     LNLHENDLLLMKLNTDLLRLTQNKISEYNQDSKLFKKNNQKQLQKELDTAKQVRSYTL
                     IFLILLMIVISLLLFNFTKVAFGYEKRLTVAKNQIKQSLDFKNRITGMISHEIRSPLN
                     IIALYSRKASATTKDNQLKETFQSIEFTTNSLLLLSNQILEYSKQEKQELKLKNKKID
                     IQNEINQIIVSMKTLLETKSNRLVVDSNLEPETFVYADVGKIYQLFYNLIGNANKFTE
                     KGIIKIKIRLTPLSDFEMNLDVLIADNGIGISENDLKNIFESYYQGTVSEKVTNLGVG
                     LGLNICKEIVTLFEGTIKIESKVEYGTKVAFNLIVTQD"
     gene            13873..14526
                     /locus_tag="PK218_06500"
     CDS             13873..14526
                     /locus_tag="PK218_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW98192.1"
                     /translation="MKTTNNHLTFLIADDHSVVRQGISLIVKELYSNAKIMQAANFKE
                     TFSILKETKIDLMILDINFPEGNSITIITDILIIQPNVKILVFTAYDENFYALRYLDA
                     GASGFLNKGCEEEEIKRAITIMFSSGKYVTKNIKEKILDSYMSKKPINPLEKLSAREV
                     EVAKLFIKGYGNLEISELLQIKKTTVSTFKIRIFEKLEIQNLPALIELFNFYYEEKK"
     gene            14653..>14999
                     /locus_tag="PK218_06505"
     CDS             14653..>14999
                     /locus_tag="PK218_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPW98193.1"
                     /translation="MNSLKSETVAPSSKEVALLDKLSLYSDLNNRLLNLVYTVAHNLN
                     GYTGNIKLLLDFIDLEESKQENNLALEHLKTVTNDLDKTITDLSQIVSLQNTLEVKKE
                     VLSLHDYIEKVDN"
BASE COUNT         5530 a         2242 c         2539 g         4688 t
ORIGIN      
        1 cagcatgctt caagttaaaa gactattatt aattgggttt ttgatgaata caacgtatca
       61 ttttgctcaa tatactgaca tcatcaattc caatcgtcct gggaaatcca tgtctgctta
      121 ttccgttggt aaaacggtta ttcaagcaga aggtggcttt tttggcatca aagaaaaaca
      181 tgatttagca cggtatgaag ctaatggttt tggttctgat ttaagcgttc gctatggtgc
      241 tttttttgag caatttgaat taatgttgga catgcaatac caaaaagatt ggtacacagc
      301 accattagtt gataaaactc gaagtggttt aagacaaaca accattggag ctaaatattt
      361 agtttatgat ccatttaaga atttaagaga agacaaacct aatttataca gttggaaagc
      421 caatcacagt aataaatttg attggaaaca gttgattcca gcagtggctc tttatgcagg
      481 tgtaaatcta agaattggca ataacccatt ttatgttgat gatgctttta taagcccaaa
      541 aggaatgatt attactcaaa accattttgg aacacattgg gtttttgtca ataattttat
      601 tttagataaa gtaacaacca aagatgcaaa tattggttta gtttcgtcct taactagagg
      661 ttttaatatg cgttggtctg ggcttttaga gtttcaagcc accaaaagcg attactatgc
      721 cgactattta tttcgtgttg gagcggccta tttaattaga gaaaacattc aaattgatgc
      781 ttcgattagt caaaatgtaa aagatacgcc ttatattctt gctggtggat ttggactttc
      841 atggcgtttt gatgctaatt atgaaacagt atatttaaga agtcaaaaac cagaagatcc
      901 aaaagagaaa gcgaaagata aaaaagcgaa gaaagacaag aaaaagaaaa gaaaagacga
      961 agttgaactt gaagaaacaa agccttaaat gattactata gttgaagcca attctcccaa
     1021 gcttttaaaa gattttgtaa agtttccatt tgatttatat aaaaaccatc cctattgggt
     1081 gccaccatta atttcagaag aattagaaac gtttgataaa actaaaaatc cagcatttac
     1141 tactgcagaa gcgacattct atttggcgta tagagaaaat aaaatcgttg gaagaattgc
     1201 tgcgattatc aattggaacg aagtgaacga ccaaaataaa aagaaagttc gctttggttg
     1261 gtgggaaacg atagatgatg ttgaagttac taaagcatta ttggaaaaag taaaggaact
     1321 tggtgaacaa aaccagttag agcatattga aggcccaatg ggattttcta atttagacaa
     1381 agtaggagtt gttaccgaag gttttgacga aataggttct atgattactt ggtataacta
     1441 tccctattat aaagagcatt tggagctatt gggttttgtt aaagaaaagg aatatcacga
     1501 aaataaattt cctttttcaa atgtaaaacc tgaattcttt tttagagtac aagaaatgat
     1561 taaaaagcgt taccaattga cgccaattaa ttttaccaaa acaaaagaca tcatgcctta
     1621 tgtggatgaa atgtttgatt tgtttaataa atcctatgct agtttagctt catttgtagc
     1681 aataacagat attcaaaaag agtattttaa aaagaaatac atcagcttta tcaatccaga
     1741 atacataaag tttattgttg ataaagatca taaaattatt gcatttagta ttgtcatgcc
     1801 aagcttttca gaagcgttac aaaaagcgaa tggaagatta tttccattag gattttatca
     1861 tttactaaaa gccaaaaaga acagtaaaga agtgttgttt tatttaattg gagttgatcc
     1921 agaatatcaa aataaaggtg ttacagcagt aatatttaac gaatattatg agtgttttaa
     1981 aacaaaagga attgaaagct gtgttcgcac accagaatta gctgacaatt acgccattca
     2041 aaacatttgg aaacatttcg acccagtagt ttttagaaaa cgctgtacat atataaagaa
     2101 cttataataa agttacactt ttatagattg tttgtcttta tacaatgaga aaatgatacc
     2161 aatagctaat gatagtgcta taaaacctaa agaaacccat tctggaaatt tgaagtaatg
     2221 aatggctaac atcttaattc caacaaaagt tagaatagct attaaactgt attctaaata
     2281 gctaaacttt tcaagcatat ttgccaaaaa gaaatacatc gaacgcaaac caagaatggc
     2341 aaagatatta gaactaaata ctaaaaatgg atcagaagta atggcaagaa ttgctggaac
     2401 actatccatg gcaaacaata catccattac ttcaataaca attaaggcaa caaacaatgg
     2461 tgttgcgaag gtaatttgtc ttttcttaat gaaaaacttt tgaccatcca tgtgcattgt
     2521 cactggaata atttttcgca tacttctaaa aatgaatgat ttttttggat taaattcttc
     2581 ttcttcacct ttgaaaagca ttttcgcagc agtaaagatt aaaaatgcac caaaaacata
     2641 ggtcatccag ctgaacttgt ttattaataa tacaccaaag taaatcatca aacctctaaa
     2701 agcaatggca ccaagaattc cccaaaacaa tactctatgc tggtattttt gtggaatttt
     2761 aaacgaagca aaaattacag caatcacaaa tatgttgtca acacttaggg ataactcaat
     2821 taaataacct gtaatgtatt ttatggatgc taatgaaggc gatagttttg tggggttttc
     2881 tataaatcca ttttgataga cccaataaat cactcctgaa aagagaaatg atattgtaac
     2941 aaaaatagca gtccattttc ctgcttcttt tgaagatata atatgcggtg ttttgttgaa
     3001 aactcctaaa tctaatgcta agaaaaataa aattgcagca agaaataata tccaaactat
     3061 cataaaaggt aatttttctc aaagataata gaaatgttta taattaaaaa aaaacctttc
     3121 aacgataaaa tttaatgttt tatcaatttt acccctaaaa aaacaaggtg tatgtatgtt
     3181 aaatacaaaa tatttatata tttgcattga aaaattaggg tataacttaa aaaatagaat
     3241 attttatggc aacattacga tttcaagcat tacacgatgc ctcttctaga aaaccggtaa
     3301 aatttgaaga atcaggaaaa aaatcagaac tttttggttc gaatgtattt aatgacaaag
     3361 caatgcgtca gtttttaact tcggatgctt ataaaggtgt aaaagatgct attcttcacg
     3421 gttctaaaat tgatagaaaa ttagcggatt atatcgcaat gggaatgaaa gaatgggcaa
     3481 taactaaagg tgtgactcat tatacacatt ggtttcagcc attaacagga gcaactgccg
     3541 aaaagcatga tgcttttttt gaaacttctt atgacggttc agatcctgtt gaaaagtttg
     3601 gtggtggtca attggtacaa caagaacctg atgcttcttc tttcccaaat ggcggaatta
     3661 gaaatacgtt tgaagctcgt ggatataccg cttgggatcc aacttctcca gcatttattt
     3721 ttggaacaac actttgtatt cctacggttt tcatttctta tacaggagaa gctttagatt
     3781 ataaaacacc attattgcgt gcgttgaatg cagtggatga agcggcaacc gatgtatgta
     3841 aatattttga taaaaacgtt aaaaaagtaa ctgcaacttt aggttgggag caagaatatt
     3901 ttcttgtaga tagtgcttta gcaaatacac gtcctgattt aattttgaca ggaagaacgt
     3961 tgttaggaca cacttcttca aaaggacaac aattagacga tcattatttt ggttccattc
     4021 catctcgtgc cttatcgtat atgcgtgaat tggaggaaga atgtatgtta ttgggaattc
     4081 cagtaaaaac tcgtcataac gaagtagcac caaaccaatt tgagctagca cccatttttg
     4141 aagaaacaaa cttagcagtt gatcacaatt cattattaat ggatgtgatg tctaaagtag
     4201 cagaacgtca cgattttaaa gtacttttcc acgaaaaacc attcaaagga gttaatggtt
     4261 ctggaaagca taacaattgg tctttggcta cagatactgg tgtaaattta ctgtcgccta
     4321 gtaaaacacc aatgagcaat ttgcagttct tagcgttctt tatcaatacg ataaaagcgg
     4381 ttcaagaata tgaagaatta ctaagagcag ctattgcaac agcaagtaat gatcatagat
     4441 taggtgcaaa tgaagcacca cctgctatta tttctgtgtt tatcggagaa caattaacta
     4501 aagttttggc tgaattagaa ggagtttcaa aaggaaaact ttctcctgag gaaaaaacag
     4561 atttaaaatt gaatgtagta ggtaaaattc cagatgtaat gttggataat accgatagaa
     4621 atagaacatc accatttgca tttacgggaa ataaatttga attccgcgca gttggttcta
     4681 cggcaaactg tgctaacgcc atgacaacgt tgaattctat tgtggctaag caattaaaag
     4741 atttcaagaa agaagttgat gcattaattg agaaaaaaga cctgaaaaaa gacgaagcaa
     4801 ttttcaatgt attaagagaa tatatcaaag aaaccaaaaa cattcttttt gaaggtgatg
     4861 gttatagcga tgcttgggaa aaagaagcta agaaaagagg tttgagcaat cataaaacta
     4921 ctccagctgc tttgaaagct aaagtttcta aaaaggcatt agatttattt gcaggattac
     4981 aagtaatgaa tcatgtggaa gttgaagcac gttatgaaat tgaattggag gaatacacca
     5041 agaaaatcca aattgaagga agggttttag gcgatattgc ccgcaatcat attattccaa
     5101 ctgctattcg ttatcaaaat actttaattg aaaacgttcg agggttaaaa gaaattttcg
     5161 gaaaagaatt tgaaaaaata gcaaaagagc aaattgtttt aatcaaagaa atatctgcac
     5221 atattgaagg aattaatact aatgtagatg ctatgattga agctcgtaaa aaagctaatg
     5281 cattaaccga tgctcaaaaa atggcggaaa tgtattgtga taaagtaaaa ccatactttg
     5341 aagtaatcag agaacattgt gataaacttg aattattggt tgacgatgaa gtctggacaa
     5401 tgactaagta tagagagttg ttgtttacaa gataaggttc attgaaacta ttatataaaa
     5461 tgccagttgt gaatattcat aactggcatt atttttttat agttgagtaa cggtataaga
     5521 tgcagaaccg ttaaagttta ttgtcaattc tactcggtaa ttttttggtg aggaagttgt
     5581 attacttatt ggaatacttg tgccattact ttctatgcta ccatcagcaa gattgtcacc
     5641 atgagttgat cctttaggat caagatatag aaattcatag cgattagtag tatttgctga
     5701 aatgcttaaa ttaaatgaaa atttatttgt agctactgaa taagtactat ttgtaaaact
     5761 tccagtaagt gaattaatta gttgcggtgc aagagaattt aatgaaagcc taaaatcatt
     5821 tactggataa gaagggttgt taaaattatt taaatcaatt aaatagaacc catttgtatc
     5881 cactaaaaaa ttagctccat taacttgaag attatttgat aaatcgactc cgtaaactgg
     5941 tgaagatgca ttgtttgtag caagaaactt taaatctgat ccggcagtta tatatttgta
     6001 gccatatctg tccaaatttc caccattatt ataattagcc attaataaag ctgttgttgg
     6061 attaaatgta ttgaaagtgc catacatgta gaaattattt ggcgaacttg gtgaagaact
     6121 tcccgaagtt ggtaacgata attgtcccgc atcatttaca gaaactacaa aaggcgttcc
     6181 atttggcgac accagtgtaa ttccttcacc aggcgaagtg attaaactat ctgcggctag
     6241 tgcataagga acactcaaca actgagttgt gccaacgttt gcaaaagttg ttccgccagt
     6301 aacatccatt tcaactttta gaaatttaga attcgtagcc caattaatag tgttaaaaac
     6361 gccagaaact actgttcctt gcccaatgat tagattgaat aaaccttgtg tattggtggt
     6421 tttcaaatgc gtttcgctgt ataaagatgt tcctgttacc gaagcgtcta aaattgataa
     6481 acgaactcta acgtttgaac ttacaacagg atttcctgct gtattcatag caattgcttg
     6541 ataagaaatt ccttgtggca cttgaccaaa agaaaataaa gtgactaaaa gtaaactaag
     6601 tgtaattatt tttttcatat tgattttttt tttaattttt ttgcacttcg actgcgctca
     6661 gtgtgacaac ttcgactgcg attagcgtga tactattttt attttttaat aattttaaac
     6721 gaattaatat ttttattttc aaattgaatc aaataaactc cagtttttaa ttcagataaa
     6781 tctagggaat tggaacttga aatttgtttt ttggcaacaa tttgtccaga tagatttaca
     6841 atggcaactt ttttattttc aagactattt tcagtttgaa acgtaataga attcatcgta
     6901 ggattgggat acacaacaat tttatcattc aaagccaatt ctggaacttc aagattttgt
     6961 tggtttactt gtgccaatat tccaataatt cccgaagtag attggttagc attttcagga
     7021 ataacaatta tttcaccaat cgaaaccgta ctgtttggtg ctaaaatgct tcccgaattt
     7081 acagattgaa ttaccgattg acaattgaca gaaaaagtgg ttgttaagaa taaaaaaagt
     7141 aaattttttt tcatgttttt ttgattttaa aatgcaaata taaacaacag ttttattaat
     7201 ttggtataaa ttttgacggt tctaaagtaa tttttttcgt aactttcaac tttaaaaaat
     7261 agaatgaaat atagaattct ttttgcgtta ctttttattt ctttttgggg ttattctcaa
     7321 ctgcagttta ctgttttttt tgaaaccaat aaataccaat taaatgatag agaggattat
     7381 aagcttcaaa gctggatttc tgaaaacaga aacaacaaag ttgttgccat tcatggtttt
     7441 accgatgaag atggtaccac cagttccaat gatactttag cccaaaaaag agtaaaagca
     7501 atttttgaaa ttataaatgg caaaatgcca attagagagg attttaaaac cataagcttt
     7561 ggtgaaaatt tcaatcaatc caagaacaaa gccgacaatc ggaaggtgac catttattat
     7621 atccttgaaa aagatttagc acgtgaaaat gaaattctag gcataaaaga agcgccaaaa
     7681 ttaattgaag ttgcaccaaa agccgaaatc aattatcccg acaaaatggt ttttgaaaac
     7741 ccaaacggaa caacctctga atttaaattg gatgttgagt ttatgaaaaa agtaggtaat
     7801 gctactgttg gcgagaaact taaaatagat aatttagagt ttatgattaa cacttttgcg
     7861 gttgtcaatg aatctcgcgg taagctttat gaattgcttt tagtattaca acacaatccg
     7921 caattaaaaa tcgaaattca aggtcatttg tgttgcatgc cagttgacag aaccgattta
     7981 tcaaccaaaa gagcaaaagc catttatagt ttcttggttg ccaatcaaat ttacccgcca
     8041 agattatcct ataaaggttt tggaagttca caacctattt ttccaattcc cgaaaaaaat
     8101 gaagccgaac gtgcagccaa ccgtagagta gaaattgtaa ttgttgccaa ttagaatgaa
     8161 attattttta gcatatttat ttttcggtgt taccactatt gcattaggtc aagaaaaaca
     8221 ttttgaagtc ttttttgatt tcaacaaaga tgtaccaact gaaaattcta tggttttatt
     8281 gaacaattgg atgaaatcct cagaaaacat agagatagta gccgtttctg gctattgcga
     8341 tagtattgat tctaatgctt ataacaaaga attggccaat aaaagaatca aaactgttct
     8401 tgaaataatt agaggaaatg ccattccaat caaacaagat ttatctattt atgtttttgg
     8461 tgaagagttt aaacaatcaa aaacacaagc tgaaaatcga aaagttatta tcgaatactt
     8521 tcaaaagttg caatccaaaa aagaagacga agcaagtaat gcaccaatgg aagccgtaac
     8581 aatcgatgct gaagataaag tggaaaccga aagaataaca ttagtagaaa aatttgaaaa
     8641 agcaaaagta ggcgatagga ttgcgatata taacatcaac ttcatgttca actctgagaa
     8701 tatcgaactt aaatcagaac cattgttgga agaattgttg tttatcatgg agcgaaatcc
     8761 caaaatggtg attaaaatcc acggtcatat ttgttgcaac ccaaatccat acaatactaa
     8821 actgtcctat cgccgcgctt tgaaaatatt taaatatctg aaagaaaacg gaattcaaca
     8881 aaaccgatta gcatataatg gagtaggaag taacaaccca atttatccca ttccagaaaa
     8941 aaatgaaaac gaacgaatag ccaacagaag agtagaaatt gaaatcgtta gaaagtaaaa
     9001 tgaaaacaat tgtattttta atactagggt tttttacaag catttgtttt gcccaaaagc
     9061 aattcgaagt attctttgat ttcaataaag acttcccaaa ccaacaatcg atacttgatt
     9121 ttaacggatg gaaagccaca tcaaacatta atgaagtaat ccgaattgaa ggctattgcg
     9181 acagtgtgga taccaaaaac tacaacaaaa aacttgccga aagacgcatc gaaagtgtgt
     9241 tgaaattatt aaaaagtagt tcggttgcca ttactaaaga tataaagaag attgctgttg
     9301 gaaaagactt tgaacaatcg caagtgcaag aagaaaaccg aaaagtaatt atatactata
     9361 ccgaaccatc actcgaaaag cccaaatctg tgctttacga atccatcaaa aatgctaaag
     9421 ttggcgaaag gataaagcta tcaaacatct atttctacaa taattctgct caaacagttt
     9481 caaaatcgca aactacttta tacgacttac tttgtgtgat ggaagacaat ccaaagttga
     9541 aaatcgaaat tcaaggacat atttgttgtc aaatagaaac cgatatcaat gatgtttcca
     9601 ccgcaagagc caaagccatt tataatttct taatcagaaa taaaatagac agaacccgaa
     9661 tgtcctacaa aggatttgga accacaagac caatttaccc tattcccgaa aaaaacgaaa
     9721 tcgaagccaa cgaaaaccga cgcgttgaaa taatgattgt tgagaattag aaggatagac
     9781 aatacacaaa ttgatgaatt gtttaaaatt ctagcaatct taaagtctct catctatcat
     9841 ctcttcgtca agtatctata aatctaaaaa aaaactatct ttgcaaaaca acatcttcct
     9901 gtcggcagac aggcaactaa ctactacaaa tgagttctga taacagccaa cgttacaatc
     9961 ttcgcggagt ttctgcttcc aaagaagacg ttcacaacgc tataaaaaac attgataaag
    10021 gtttatttcc taaagctttt tgcaaaattg ttcccgatta tttaaccaac gacgatgact
    10081 attgtttgat catgcacgcc gatggtgcgg gtacaaaatc gtctttagcg tatctgtatt
    10141 ggaaagaaac gggtgatata tccgtttgga aaggcattgc acaagatgcg ttgattatga
    10201 atattgacga tttattgtgc gtgggtgcta cagacaatat catgctttct tccaccattg
    10261 gcagaaacaa aaacctaatt ccaagcgaag tactttcggc tattattaat ggcaccgaag
    10321 aactgattgc cgatttgaaa aagtttggtg taaccattca ttccactggt ggcgaaacgg
    10381 ctgatgtggg tgatttggtg cgtaccatta ttgtagattc taccgttacg gcgcgtatga
    10441 aacgcagcga tgtgattgac aatgccaaca ttcaagcagg cgatgttatt gttgggatgg
    10501 cttcttttgg gcaagctaat tatgaaaccc aatacaacgg cggcatggga agcaacggtc
    10561 taacttcggc acggcacgat gtgtttgcca aatatcttgc cgataaatac cctgaaagtt
    10621 atgataattc agtacctaca gacttagttt attctggaaa tattaaacta actgatgctg
    10681 tggaaaacgc accgttggat gctggaaaat tggtcttatc gccaacgcga acctatgcgc
    10741 ctatcatcaa agcgatttta gataaataca caccaaacga cattcacggc atggtgcatt
    10801 gttcgggtgg tgcgcaaacc aaggtgttgc attttataga caacgtacat gttattaaag
    10861 acaatctgtt tccaattcca cctttatttc aattgataca agcgcaatct aaaaccgatt
    10921 ggaaagaaat gtaccaggtc ttcaactgtg gtcaccgcat ggaagtgtat gtaccacaag
    10981 aaattgccaa cgatattatc gaaatctcta aatcctttgg tgttgatgct caaatagtgg
    11041 gtagcgtagc agcaaattca accaaaaaac ttaccattaa aagtgagttt ggagtttttg
    11101 agtattaaag atttaaaatc tccatcttag gcctgaaaag acaatgtact aattaacgag
    11161 gagaattaga acacggatat aacggatgct tggcaacgtg gatggctacg gattatttta
    11221 gtgccgtttt gatgaatgtt cttttgaata tctctccata ttttacactt ttgtttaaac
    11281 ctattttagg acaagacagg gtatagtaga aactttattc cgctttattt tcccaaaaat
    11341 aaccaaaaaa ccaattcgct taacaaattg ctttacaatt tgttaaaaat aattcctact
    11401 catagcttct tgaggtttta gatgtctttt tggttttgag tgggttataa aaggttcttt
    11461 tttattgagt cagggattat ttttagtatg catggacttt tttgtattta tgctatcaac
    11521 tcagcaagtg tttcgcctat aagcgacatt cgcaaactga tggcagccta aattttattt
    11581 tatttttttg taaaaaactt tttagaaccc gtctgaaggt ttttttatta acttgcaatg
    11641 tgcttttgaa aaaaaaatta aagtaattaa ttgtagaaat gaaatgtgta acgagtcatg
    11701 aaaatgaagt ctataaaaaa caacaacaac ttactaactc atttgcaatg cttaatagat
    11761 agtgtagaat taattctaca taaaaaatta cagagtacta cattgttgtg tgataagaat
    11821 actaaatttg ttctataatt tagaagtgct tttcaatgca tttctttaga aataaaaaga
    11881 aacccccaaa tctttttaac attaaagtaa tatttttaaa gttaagctta ttaaagtggt
    11941 aactaactta attttagttt ttagtgtaat ttactaatgc aatttaaaga taaagcttta
    12001 tgttttttga tgtaatttaa taatgaacag cgttattttt tattcttagg aaatcaataa
    12061 aaatagagag taaatatatt tttgagtagt tggactaatt actcaaacag attttgcaat
    12121 tttgttttaa aaccaataaa aaatagcccc aatttttgac catttgaaac gactaaaatt
    12181 tgaacatcga aagttaattc attacgcttt gttaacttgt attgttttgt tacagataat
    12241 tgccatcata atttggtata acgaaacaaa gcatacagaa gcttatgaca acctaaaggc
    12301 taccaacaaa attttgagct atacagatag tttgaataca aatttgttgc attcacaagt
    12361 gttgtttact acttataggc tcaataatga tagcgatata ttgaccaact atggtgtttc
    12421 tttagaaaaa gcaaaccgat atttagatag tttaaaatcg gaaacaaaaa acaaaaacct
    12481 attaaaaaaa gtagtaaata aaaagcaaaa atcagaagtt gaaattagaa atattaaaaa
    12541 atcaatcgat tctattatcc atttgcaaac tactttttca attgaaaata atctaaaacc
    12601 aatcaacctt aaaccattcg aactcaaaaa tgtattagac aatgtaaaag aagattctta
    12661 tatcaaaatt gatagtgttt caaagaaaag acttttttca agattgagtg atgcttttgc
    12721 aggaagagta agtgttcaaa aagaatattt gaatacagtg gttaccattc agtataaaga
    12781 taaagtttct aaaggtactt ttgaagaaca aatagctact gcaattggat attcgaataa
    12841 ctattattca aaacaaattt cgcagctgca acagtctttt ttaaatcttc acgaaaatga
    12901 tttgttatta atgaagttga atactgattt attgagatta acacaaaata aaatttctga
    12961 atacaaccag gattcgaaac tttttaaaaa aaataatcaa aagcaacttc aaaaagaact
    13021 agacaccgca aaacaagtaa gaagctatac cttgattttt ttaattcttt taatgattgt
    13081 aatttcttta ttgttattta attttaccaa agttgctttt gggtatgaaa aaagattgac
    13141 ggttgccaaa aatcaaatca aacaaagtct tgattttaaa aatagaatta cgggaatgat
    13201 tagccacgaa attcgatcgc cattaaacat tattgcactt tatagccgaa aagcgagtgc
    13261 aaccactaaa gacaatcaac ttaaagaaac attccaatct attgagttta ctaccaattc
    13321 cttactgctg ttatccaacc aaatattaga atattctaaa caagaaaaac aagaattaaa
    13381 attgaagaac aaaaaaattg acattcaaaa tgaaatcaat caaattattg tttcgatgaa
    13441 aaccttgctc gaaaccaaaa gcaatagact tgttgttgat tctaatttag aacccgaaac
    13501 ttttgtatat gctgatgttg gaaagattta tcaattgttt tataatctca ttggaaatgc
    13561 taataaattt accgaaaaag gaattataaa aataaaaatc aggttgactc ctctttcgga
    13621 tttcgaaatg aatctggatg tattaattgc cgacaatggt attggtatct ccgaaaatga
    13681 tttaaaaaac atttttgaat cgtattatca aggtactgtt tccgaaaaag taaccaatct
    13741 tggtgttggt ttagggttaa atatttgcaa agaaatagta acactttttg aaggaacaat
    13801 aaaaattgaa agcaaagtag agtatggtac aaaagttgcc tttaatttaa ttgtaactca
    13861 ggattgataa aaatgaaaac aacaaataac catcttactt ttttaattgc agacgaccat
    13921 agtgttgtcc gacaaggaat ttctttaata gtcaaagagt tgtatagcaa tgcaaaaata
    13981 atgcaagcag ctaactttaa agaaactttt tctatactta aagaaaccaa aattgacctg
    14041 atgattttgg atattaattt tcccgaaggc aatagtatta ctataattac agatattctt
    14101 attattcaac ctaatgtaaa aatattggtt tttacggcct atgatgaaaa tttttatgca
    14161 ttgcgttatt tagacgctgg tgcttccggg tttttaaaca aaggttgtga ggaagaagaa
    14221 atcaaacgtg ccattaccat tatgtttagt agtggaaagt atgtaaccaa aaacattaaa
    14281 gaaaaaattc ttgactctta tatgtccaaa aagccaataa atcctttaga gaaactttcg
    14341 gctagagaag tagaagtagc caagctcttc ataaaaggtt atggtaatct cgaaatttct
    14401 gaattgctcc aaataaaaaa gactacagta agtactttta aaattagaat atttgaaaaa
    14461 ctagaaattc aaaaccttcc cgctctaatt gaattgttta acttttatta tgaagaaaaa
    14521 aaataagctg ttgatgtaga aacaattcta caaaaacgac agcgttattc tacaaattgt
    14581 tgtttttctt gaaattatat ttgcattgtt attcatagag aattacagct ggatatataa
    14641 aaaaaaaata aaatgaacag tttgaaaagc gaaaccgttg caccaagctc taaagaagta
    14701 gcgttgttgg acaaattgtc tttgtatagc gatttgaata atcgcttatt aaatctagtc
    14761 tatactgttg cacacaattt gaatggatat acaggaaaca tcaagttact tttagatttt
    14821 attgaccttg aagaaagtaa gcaagaaaat aatttagcac tagaacattt aaaaacggta
    14881 acaaatgatt tagacaaaac aattactgat ttatctcaaa tagtatcgtt gcaaaatact
    14941 ttggaagtta aaaaagaagt attgtcgctt catgattata tcgaaaaagt tgacaatat
//
LOCUS       DAOOBH010000049        14261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5505_length_14261_cov_6.007250, whole genome shotgun sequence.
ACCESSION   DAOOBH010000049 DAOOBH010000000
VERSION     DAOOBH010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14261
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5505_length_14261_cov_6.007250"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PK218_06510"
     CDS             <1..550
                     /locus_tag="PK218_06510"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HPW98194.1"
                     /translation="VDTVKIENGKFVFKGSAKEPELYLVQVEKVQGKVPFILENGDIE
                     MTINKDSVAITKVSGTYNNDELTSYKENGMKIQKKMMKFQEDNMAKMNQAQQKNDTVV
                     MNSLRNEFKALRAESTNYMNNYPKEHPKSFISVLLVENMLNNPDVKVADAEKIFNSLE
                     ESLRKSAAGKKVTEAIATQKKK"
     gene            589..996
                     /locus_tag="PK218_06515"
                     /pseudo
     CDS             589..996
                     /locus_tag="PK218_06515"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
     gene            1148..1229
                     /locus_tag="PK218_06520"
     tRNA            1148..1229
                     /locus_tag="PK218_06520"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1182..1184,aa:Tyr,seq:gta)
     gene            1300..1827
                     /locus_tag="PK218_06525"
     CDS             1300..1827
                     /locus_tag="PK218_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98195.1"
                     /translation="MISNLKLVAVSLTLSIVFISCKKEEETVVSDTPKEIILPRVQAI
                     PTGNYVAPPPQQQQVQQQANPNNQVVTQTPVATKPGMNPPHGQAGHRCDIQVGAPLNS
                     KPAAPVVQSGKAANQTITVPNNAQKTLSNPNATPLMLDPNVNPVVTAPGMNPPHGQEG
                     HDCAVAVGAPLPTKQ"
     gene            complement(1932..2627)
                     /locus_tag="PK218_06530"
     CDS             complement(1932..2627)
                     /locus_tag="PK218_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496643.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPW98196.1"
                     /translation="MKKSIALVALLFTVVAFAQDNKAQVPQISVTGEGKVKVTPDRAI
                     INVGVLNTGKDAKEVKSLNDETVDKVIKFLKKSGLNAADYKTNNVSLHKSYDYEKKKQ
                     VYQANQSISITLKDLSKYDEIMMGLNDTGVNNIQGVEFKSSKMEELEKEARKKAMLNA
                     KQKAEDYVSVLGQKVGKALLISDSSQAYYPQPMYKNSMMGGEAMVSGDSQETLAVGEI
                     EIIATVNVTFILD"
     gene            complement(2676..3317)
                     /locus_tag="PK218_06535"
     CDS             complement(2676..3317)
                     /locus_tag="PK218_06535"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPW98197.1"
                     /translation="MENMFLYGLIAVNAIVSFKGFEDQFFFRKYQFHMGSIRAGEQYR
                     VVTSGFLHADIGHLAFNMITLYFFAPIVSQSLGDFSFLLIYFASLVCGSLLTMVFHGN
                     EYSYSAVGASGAVTGILYSAILMYPQMTIGIFGIIPMPAYIFGIGYLLYSIYGMRAKN
                     DNIGHTAHFGGAIGGYVITLLKVPTLFQDNLLMVILLAVPIVLLFILAKAGKL"
     gene            complement(3327..4811)
                     /locus_tag="PK218_06540"
     CDS             complement(3327..4811)
                     /locus_tag="PK218_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPW98198.1"
                     /translation="MQATQLQSELEVYFEKFRNNIIGIDQEFQSPFGTQKIIYTDWTA
                     SGRLYRPIEEKMTNVFGPFVANTHTETTISGTAMTMAYHEARSIIKRHVNANDNDVLI
                     TDGTGMTGVVNKLQRILGLRIAENLKEYTSIPDAIKPIVFISHMEHHSNQTSWLETIA
                     DVVVIDCNEEGLFCLENFKKTLEQYKHRSFKIASITSCSNVTGIQTPYHEVAKIMHQN
                     NGVCFVDFACSGPYVEIDMHPDEESYLDAIFFSPHKFLGGPGTTGVMLFNKNLYKNNI
                     PDCPGGGTVSWTNPWGEHKYIDNIEDREDGGTPGFLQVIKTALAIQLKEEMGVQNIIK
                     REHEIVDYIFSRLNNVEDLNILANQHQDRLGVLSFYINDLHFNLGVKILNDKFGIQSR
                     GGCSCAGTYGHFLLHVDQETSNNLVSQISLGELLKKPGWIRMSIHPTTTNQEIEYVCD
                     SIIALAKNHKEWSKNYQYDSKSNNFHHENDPLLEKEMVQNWFKT"
     gene            5250..5789
                     /locus_tag="PK218_06545"
     CDS             5250..5789
                     /locus_tag="PK218_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPW98199.1"
                     /translation="MRHQKVRFKVVFYLKYYFARHWSLSARIKYYETGVSFYKPDTHG
                     GSWIDFGSDESYGSFNGAVICVPIDVKWEFRIYKNLGGSLKIGAAYTMESKSNYGNYS
                     ANLQPIDYPKQYGSINSGYGLNYFINKKLAVYLDFEYYSGDSKGFSDNLLGRRSYNVE
                     NNLVNFAVKYNFKKDKAIN"
     gene            5960..6448
                     /locus_tag="PK218_06550"
     CDS             5960..6448
                     /locus_tag="PK218_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98200.1"
                     /translation="MKKIRIVGLVLILIGFKSCKKNEDKKETEKTNTETEQLATEQCY
                     KAVYEKDTLDLKITPLKDGKIVGSLVMKVFDEPKREGKIEGEFKGDTLFCSYTFILTK
                     DVTKTYKNPMAFLKQGDELILGNGEIEMYLGASYFKKDKPIDFEKVKYKFASVDCSTK
                     TN"
     gene            complement(6578..7474)
                     /locus_tag="PK218_06555"
     CDS             complement(6578..7474)
                     /locus_tag="PK218_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98201.1"
                     /translation="MKIFSWKIISLFVITLSLGWTTEMGSGYNIQKPNTTFVLPDILH
                     EISGLTDIDQRTIACIQDENGIIFIYDIVLNKIKKQYNFHLDGDYEGITKVGTTMYVL
                     RSDGVLFEFLNYESPNFKLKTYQTGIPAHNNEGLCYDADSNILLIANKGKSVKGKEFK
                     DKRAIYGFDLKTKKLTTSPVYEFDINVIKKHVADKKIKVPTKLKKGENPEPFIKLMTS
                     AIAIHPMSKKLYVLSAADHLFFVFNRNGSIHHIEPLSTTVFNKAEGITFLENGDMLIT
                     NEGQQKKPTFLRFNYKPTGVKK"
     gene            7680..9074
                     /locus_tag="PK218_06560"
     CDS             7680..9074
                     /locus_tag="PK218_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HPW98202.1"
                     /translation="MNRNTLWSLRLGFSGKEAQKIETLGLQKFLEQSFATKVDKKEPD
                     LLVDSPKTKAELKAARQLMKTTDDEDAVKKILRKEIQVSQKLKEWWLEKMMDEAFPLR
                     EKMTCFWHNHFVSTYQKVKVNHWVFQHNQLLREQAFGNFKTLTKAVLKTNAMVRYLDN
                     VDNRRDKINENLSRELLELFTLGIGNYTEEDIKNGAKGLAGLTIGETNANYRKLLENN
                     DTITYFCKTGTFKADELVDIIFEQKAIPYFVTRKILKWFLYDKPSEELVTYYGDYFRK
                     VNFELQPLLTKIFTEEYQKNISGSKIKDPLVYILQLKYELKIEKMNTRVLTFFLKQQG
                     MDLFNQPNVKGWNGGNEWLSSQMYLQRNNVADLLCSGQPLNRRRKYMSDEMMDENSDT
                     MLRVSIDFDANGTNKTIIAQLSERVLFQTDDSMQKDFEELLKYDFNPKSEGAENAVLR
                     LFNTMVKLPEFQLI"
     gene            9077..10243
                     /locus_tag="PK218_06565"
     CDS             9077..10243
                     /locus_tag="PK218_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HPW98203.1"
                     /translation="MNRRNFLSLTGTFTGGSLLLPSFLHAFGQQPSLVIGEQCLVFIQ
                     LNGGNDGLNTFVPYDNPLYYDLRSKIALSKDVLLGKNNGMGFHPALKDFANMQQNGDL
                     SVLQNVGYPEPVRSHFRSQEIWQTATDSNTYANNGWLGKYLDLKCNDHEPTAGINLDN
                     IDNLALKGDEPNSITVKDPNRFKIKNEKEEVGKLSDNPQLDFVRKIANSVMEGSEDIQ
                     KALAKSTAEVSYPKTNLGKNLEWIARLVKGNLNSKVYYTSQNGYDTHDNQLNIQNRNL
                     TELNDAVASFYSDLKNAKLMQNVTVVVFSEFGRRVKDNGSGTDHGTAAPMFVIGGSNK
                     GKVFGNNPNLSDLDNGDLKHEIDFRSVYASLLHDKMNFDARKIGIKNAGFKGLF"
     gene            complement(10245..10973)
                     /locus_tag="PK218_06570"
     CDS             complement(10245..10973)
                     /locus_tag="PK218_06570"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPW98204.1"
                     /translation="MLIIKLNATDSTNDYLKQLNKEKPLDNFTTVTTVSQTKGKGQMG
                     AQWTSQPGKNLTFSLLYKPKKQFCTSIFDVNVTIAVSIIEALETFEIPKLNIKWPNDI
                     LAENKKIGGILIENSLKADATIDTIIGIGINVNQESFPDFIQASSLKKITGLEYDLEI
                     LLDKITNTIQQNLEEIDSQKEFFWKQYHQHLFKHNQPMAFENTSQEKFMGIIKNVSTD
                     GKLQIQLEDDSIQSFGLKEIKMLY"
     gene            11069..11440
                     /gene="rsfS"
                     /locus_tag="PK218_06575"
     CDS             11069..11440
                     /gene="rsfS"
                     /locus_tag="PK218_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPW98205.1"
                     /translation="MAKREVQSDILLANIIKGIEEVKGNDIDILDLREIDTAVCDYFI
                     ICNGNSNTQVNAIVNSIQKLVSKELKDKPWHVEGTDNGEWVLMDYVNIVVHVFQKQIR
                     EFYNIEGLWGDAKITTIPTNY"
     gene            11459..13381
                     /gene="ftsH"
                     /locus_tag="PK218_06580"
     CDS             11459..13381
                     /gene="ftsH"
                     /locus_tag="PK218_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPW98206.1"
                     /translation="MANEKKQNNFKFSPYWIYGLLIGVFLLLNVFGGGSFQEVGKITS
                     SKFNTMLDSGKVDKVIVYNKTEADVYLTAAAMKEKENKKVASDVLGRENKGPHYSFEI
                     GNDELFQKKLEEAKSENKLKSFDFKQRGEWTGILVSILPFVFIIGLWFFIMRRMSGGS
                     GGGGGQLFNIGKSKAKLFDEKTDVKITFKDVAGLEGAKEEVQEIVEFLRNPEKYTKLG
                     GKIPKGALLVGPPGTGKTLLAKAVAGEAKVPFFSLSGSDFVEMFVGVGASRVRDLFKQ
                     AKEKSPAIIFIDEIDAVGRARGKNNMSGSNDERENTLNQLLTEMDGFGTNTHVIVLAA
                     TNRADVLDKALMRAGRFDRQIYVDLPDVRERKEIFEVHLAPLKKVDNLDLDFLAKQTP
                     GFSGADIANVCNEAALIAARQNKEAVDKQDFLDAVDRIVGGLEKKNKIVTKDEKRAIA
                     VHEAGHATVSWMLEHAAPLVKVTIVPRGQSLGAAWYLPEERLIVRTDQMLDEMCATMG
                     GRAAEKVVFDKISTGALSDLEKVTKQARAMVTIYGLNEKLGNITYYDSSGQSEYSFSK
                     PYSEETALVIDKEINTLIENEYQRAIKLLEENKDKLLKLADILIEKEVIFKDDLETIF
                     GKRPFDKHEDELVADA"
     gene            13493..14098
                     /locus_tag="PK218_06585"
     CDS             13493..14098
                     /locus_tag="PK218_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B/C"
                     /protein_id="HPW98207.1"
                     /translation="MSLFRKIFGSSNDASDDDNQSEFNNIPSSFSLLPVDEKFTYNFK
                     KNGGKFIYCEDLGEVKEQFLNILEENDWFECEAMCIEPKLFSMLDENKLQYKDVENPK
                     FLFATCENLIAEEGSVLFSSNQIKQHKPNDLPVNIVILAKTSQIIESKSDGLRGIKKN
                     YDKDYPNNITTIKYFEKAKDEDFLQYGSVAKNLYLLLLEDL"
BASE COUNT         4812 a         2193 c         2762 g         4494 t
ORIGIN      
        1 cgtagatact gtcaaaattg aaaatggtaa gtttgttttt aaaggttcgg ctaaagagcc
       61 tgaattgtat ttagttcaag tagagaaagt acaaggaaaa gttcctttta tattagaaaa
      121 tggtgatatc gaaatgacca taaacaagga tagtgttgct attaccaaag tttcaggtac
      181 ttataataat gatgaactta ctagctacaa agaaaatgga atgaaaattc aaaagaaaat
      241 gatgaaattc caggaagata atatggctaa aatgaatcag gcacaacaaa aaaatgatac
      301 agttgtaatg aattctttaa gaaatgaatt caaagcttta agagcagaaa gtacaaatta
      361 catgaacaac tatccaaaag aacacccgaa atcatttatt agtgttttat tagttgaaaa
      421 catgttgaat aatcccgatg ttaaggtagc agatgctgaa aaaatattca attcattgga
      481 agaatcgtta agaaaatcag cagcaggtaa aaaagttacg gaagctattg caactcaaaa
      541 aaaaaagtag ttgaagttaa aaagacagca acaacagctg gcgaagtagc accgaacttt
      601 caaggaaaat ctccagaagg aaagacaatt tctttaaaag aaagtatggg taaagtaact
      661 ataattgact tttgggcttc ttggtgcaaa ccttgtcgtt tagaaaatcc taatgtagta
      721 gcactatata gcgagtttca ttcaaaagga ttaaatatcg ttggtgtatc tttagacgaa
      781 gatattacta aatggaaaga agccataaaa aaagataaca ttacttggac acaactttca
      841 aacctaaaag gctggaaaga tccaattgca gaacaatata atatttccca aattccaact
      901 acaatcctat tggatgctaa cggaaacatt gttgcaaaag atttaagagg tgaggctttg
      961 aaagctaaag ttgccgaatt attagcgata aaatagtgta aaatatagat gcaataaaaa
     1021 aatcttcttc ggaagatttt tttatttagt tcgttttcaa agaattaaat tttgattgaa
     1081 aaatatattt ttattttgtt tgcatctctc aaaacactta ttatatttgc accctctata
     1141 caggtttggg gagatactca agcggccaac gagggcggac tgtaaatccg ctgattacat
     1201 cttcgcaggt tcgaatcctg ctctccccac aaaaagcatc atacttatga tgcttttttt
     1261 atttagaaaa gctatatttg aaattctaaa aaaattagta tgatatcgaa tttaaaatta
     1321 gtagctgtta gcttaacttt atcaatcgtt tttatttcgt gtaaaaaaga agaagaaact
     1381 gttgtatccg acacacctaa agaaattatc ttgccaagag ttcaagccat tccaactgga
     1441 aattatgttg caccaccacc acagcagcaa caagtacaac aacaagccaa cccaaataat
     1501 caggtagtaa cgcaaacacc tgtagcaact aaaccaggaa tgaatccacc acacggacaa
     1561 gcgggacatc gttgcgacat acaagtaggt gcaccattaa attcaaaacc tgctgctcca
     1621 gtagttcaat caggaaaagc ggcaaaccaa acaattactg ttcctaataa tgctcagaaa
     1681 acactatcaa atcctaatgc tacaccttta atgttggatc ctaatgtaaa tccagtagtt
     1741 actgctccag gaatgaatcc accgcatggt caagaaggtc atgattgtgc tgtagccgtt
     1801 ggcgctccgt taccaacgaa acaataagta tagaataaag cctccaattg tacatgcccc
     1861 aaaaagttag aaactatttg gggcattttg agtgattttc ttttgaaaat cgtattgaga
     1921 ttgctcttta tttaatctaa tataaaagta acatttacag tagcaataat ttctatttca
     1981 ccaacggcta atgtttcttg actatcgcca cttaccattg cttctccacc catcatactg
     2041 tttttataca ttggttgcgg ataataggct tgagaactgt ctgagattaa taatgctttg
     2101 cccacttttt gtcctaaaac cgaaacataa tcttctgctt tttgtttcgc attcaacatc
     2161 gctttctttc tggcttcttt ttccaattct tccattttag acgatttaaa ttctacgcct
     2221 tgtatgttgt ttactccagt atcattcaat cccatcatga tttcgtcata ctttgacaag
     2281 tcttttaacg tgatgcttat tgattgattg gcttgataaa cttgtttctt tttttcataa
     2341 tcatagcttt tgtgcaagct tacattgtta gtcttataat ctgcagcatt aagaccgctt
     2401 ttctttaaaa acttgattac tttatctacc gtttcgtcat tcaaggattt tacttctttg
     2461 gcgtcttttc cagtatttaa aacacctaca ttaataatgg ctctgtctgg agttactttt
     2521 acttttcctt caccagttac ggatatttga ggaacttgtg ctttattatc ttgtgcaaaa
     2581 gcaacaacag taaataataa agcaactaat gcaattgatt ttttcatgat gatatgtatt
     2641 taaatttaga actagaaaaa caaaagttgt gccaattata gtttaccagc tttggctaag
     2701 ataaacaata atactattgg aacggctaat aaaataacca tcaacagatt gtcttgaaac
     2761 agtgttggca cttttaacaa agtaataaca tagccaccaa tagcaccacc aaagtgtgcc
     2821 gtgtgaccaa tattatcgtt cttggcacgc ataccataaa tagaatataa caaataccca
     2881 ataccgaaga tgtaagcagg catggggata attccaaaga tgccaatcgt catttgagga
     2941 tacattaata tagctgaata tagaattccg gttactgctc ccgaagctcc aacggcacta
     3001 taggaatact cattgccgtg aaacaccatc gttaataaac taccgcaaac caaactggcg
     3061 aaataaataa gtagaaacga aaaatcgccc aaagactgtg atactatagg tgcaaagaaa
     3121 tagagcgtaa tcatgttgaa cgccaaatgt ccaatatcgg catgaagaaa gcccgaagta
     3181 accacacgat attgttctcc cgcgcgaatg cttcccatgt gaaattggta ttttctaaaa
     3241 aaaaattggt cttcaaaacc tttaaaactt actattgcgt taacggcgat taaaccataa
     3301 agaaacatgt tttccataaa gttgttttag gttttaaacc agttttgaac catttctttt
     3361 tcaagcagtg ggtcgttttc gtgatgaaaa ttattgcttt tgctatcata ttgatagttt
     3421 ttgctccatt ctttatgatt ctttgctaaa gcaatgatgc tatcacaaac gtattctatt
     3481 tcttgattgg tagttgttgg gtgaatagac attctaatcc atcccggttt ctttaatagt
     3541 tcgcccaaag agatttgaga aactaaattg tttgaggttt cttggtcaac gtgtaatagg
     3601 aaatgcccat aggttccggc acaactgcat ccaccacggg attgaatccc aaatttgtcg
     3661 tttaatattt ttacccctag gttgaaatgt aaatcgttga tgtagaaaga tagtactccc
     3721 aatctgtctt gatgttggtt ggcaaggatg tttaaatcct ctacattgtt caatctactg
     3781 aaaatgtagt ccacaatttc atgctcgcgc ttgatgatgt tttgcacacc catttcttct
     3841 ttcaactgaa tggccaatgc tgttttgata acttgaagaa aaccaggagt tccgccgtct
     3901 tcgcggtctt cgatgttgtc tatatatttg tgttcacccc acggattggt ccagcttacg
     3961 gttcctccgc cgggacagtc gggaatattg tttttgtata agtttttgtt gaacagcata
     4021 acacccgttg ttcctggtcc gccaaggaat ttgtgtggcg agaagaatat ggcatccaaa
     4081 tagctttcct catcgggatg catatcgatt tcaacatacg gtccagagca agcaaagtca
     4141 acaaagcata caccgttgtt ttggtgcatg atttttgcca cttcgtggta aggtgtttgt
     4201 attccagtaa cattggagca agaggtaatg gaggcaatct tgaaacttct atgtttgtat
     4261 tgttccaaag tctttttaaa attttctaga cagaataacc cttcttcgtt gcaatcgatt
     4321 accactacat cggcgatggt ttctagccat gaggtttggt tagaatggtg ctccatgtgc
     4381 gatataaaca cgatgggctt gatggcatcg ggaatgctgg tatattcttt taggttttcg
     4441 gctatccgca atcctagtat gcgttgcagt ttgttgacca cgcctgtcat accagtgccg
     4501 tcggtgatta gtacgtcgtt atcgttggcg ttaacgtggc gtttgatgat gcttcgtgct
     4561 tcgtggtacg ccattgtcat ggcggttcct gagatggtag tttcggtatg ggtgttggct
     4621 acaaaaggtc caaaaacatt cgtcattttt tcctcaattg ggcggtatag tcttccgctg
     4681 gcggtccagt cggtgtagat tattttttgg gtgccaaatg gtgattgaaa ttcttggtct
     4741 atcccgatga tgttgttgcg gaatttttca aaatagactt ctaattccga ttgtaattgt
     4801 gttgcttgca tgccctaaat tgtttagagt tcaaagataa agatttttat gggaaatgtt
     4861 ttttttgttt gacggtttgt tggaacgcgg ttaaatcgga ttgacggtgc caaacgcaaa
     4921 taaatacgga ttttaattct tggagaaagg aacacagatt aaagaaattg aagaaatctt
     4981 ttttgaaatg tgggaaaccg taatttttgg gttactattt ttattacatt cgtattttta
     5041 aagtaataaa aataataatt tttgaatgat tattaaaaga aatcaatgtt aacttaaaaa
     5101 agtaaatatg aaaagtaaaa tatttattat tgccctactg agtttgtttt caaatagaat
     5161 tatttctcaa acaaatgaaa atataaatcc aaaagggaat tggttttttg gtgtagaaat
     5221 tggaataaat aaaattagtt ctttttcaaa tgaggcatca aaaagttcgt ttcaaggtgg
     5281 ttttttatct gaaatattat tttgcaagac attggagttt gtctgctaga attaaatatt
     5341 atgaaactgg agtttcgttt tataaaccag atactcatgg tggaagttgg attgattttg
     5401 gttcagatga aagttatggt agttttaatg gagccgttat ttgtgttcca attgatgtca
     5461 aatgggagtt tagaatttat aaaaatttag gaggtagtct aaaaattggg gctgcttata
     5521 ctatggaaag taaaagtaat tatggaaatt actcagcaaa tttacaacca atagattacc
     5581 caaaacaata tggtagtatt aattccggtt atggtttgaa ttattttatc aataaaaaat
     5641 tggctgtgta tttagatttt gaatattaca gtggtgattc aaagggattt agtgataatc
     5701 ttctgggaag aagatcgtat aatgttgaaa ataacctcgt aaattttgca gttaaatata
     5761 attttaaaaa agataaagcg ataaattaag acaattgact gcttactgaa attaaatgca
     5821 taagcagttg cacaaacgtg tacttatagt aagtgcaatt ggcaaaacat tttaaatcac
     5881 taaattttta atactactca ataattaatt cgtacattta tatttattaa tactcattta
     5941 gtaactaaat tttattgaca tgaaaaaaat aagaatagta ggtttggttt taattttaat
     6001 tggttttaag agttgtaaaa agaatgaaga taaaaaagaa actgagaaga caaacacaga
     6061 aacagaacaa ctagctaccg aacaatgcta caaagcggtg tatgaaaaag acacactaga
     6121 tttaaaaatt acgccattaa aagatgggaa aatagtaggt agtttggtga tgaaggtttt
     6181 tgatgaacct aaaagagaag gtaaaattga aggcgagttt aaaggagata ctttgttttg
     6241 ttcctataca ttcatactta ctaaggatgt aactaaaacg tataaaaatc cgatggcatt
     6301 tttaaaacaa ggcgacgaac tcattttagg caatggagaa attgaaatgt atctaggggc
     6361 ttcctatttt aaaaaagata aacctattga ttttgaaaag gtgaaataca aatttgcttc
     6421 ggttgactgt agtactaaaa caaactaata caaaacaaaa cgccagaaaa aggatgttgt
     6481 gtgatgagcg atttgtgccc ttcgactgcg ctcagggtta ctaagtgggt tgttggtttg
     6541 tgttgttgcg aaattggagt agcaatgttg ttgcaaacta ctttttaact ccagttggtt
     6601 tatagttaaa tcgaagaaag gttggctttt tttgttgtcc ttcgttggta ataagcatgt
     6661 cgccattttc taaaaaagta atgccttctg ccttgttaaa aacagttgtg cttaaaggtt
     6721 caatgtgatg tatgcttcca tttctattaa acacaaaaaa caaatggtcg gcagccgaaa
     6781 gtacatagag tttttttgac atgggatgaa tagcaattgc tgaggtcata agtttaataa
     6841 aaggttctgg gttttcccct ttttttagtt ttgtaggtac ttttattttc ttgtcggcaa
     6901 catgtttttt aataacatta atatcaaatt cataaacggg agaagtggtt agttttttgg
     6961 tttttaaatc aaatccataa atagctcttt tgtctttaaa ctctttgcct tttacggatt
     7021 ttcctttgtt ggctatcaat agtatgttgc tgtcagcatc atagcaaagt ccttcgttgt
     7081 tgtgggcagg aattcctgtt tgataggttt ttaatttaaa atttggactt tcataattca
     7141 aaaactcaaa taaaacacca tcacttctta gtacatacat tgtggtgcct accttagtta
     7201 tgccttcata atcgccatca agatggaaat tatattgttt cttgatttta ttcagaacaa
     7261 tatcatagat aaaaataata ccattttcgt cttgaataca ggcaatagtt ctttggtcaa
     7321 tatctgtcaa tccagagatt tcatgaagaa tatcaggaag cacaaatgtg gtattgggtt
     7381 tctggatgtt gtaccccgaa cccatttcgg ttgtccagcc taatgataaa gttataacga
     7441 ataaactaat aattttccaa ctaaatattt tcatagcact ttgattgctt taattctcca
     7501 atgttcaaag atactaaact aagtttaatt gatttgtaat gaatagattc gggcgatgtt
     7561 gcgtagctgc agttaatcgc atctctttac tgaaaaacag atttggacaa aattacttaa
     7621 gcataattaa accttatttg atatcaaaag tcttaacttt aaattttcaa attttggtta
     7681 tgaatcgcaa tacgctttgg tcacttcgct tgggtttttc gggaaaagaa gcacaaaaaa
     7741 tagaaacatt aggacttcaa aagtttttgg agcaatcgtt tgccaccaaa gtagataaaa
     7801 aagaacccga tttgttggtc gatagcccta aaactaaagc agaattgaaa gctgctcgtc
     7861 aactgatgaa aacgactgat gatgaagacg ctgttaaaaa aatattgcga aaagaaattc
     7921 aggtgtcgca aaaactaaaa gaatggtggc tcgaaaaaat gatggatgag gcttttccgc
     7981 ttcgcgaaaa gatgacttgt ttttggcaca atcattttgt atctacctat cagaaagtaa
     8041 aagtaaacca ttgggtcttt cagcacaatc agttgttgcg cgagcaagcg tttggtaatt
     8101 ttaaaacctt gaccaaagcg gtgctaaaaa ctaatgcaat ggtgcgctat ctggacaatg
     8161 tggataatag acgagataaa attaatgaaa accttagtag ggaattgttg gagttgttca
     8221 cgctaggtat tggcaattat acggaggaag acattaagaa tggtgctaaa ggattggcgg
     8281 gtttaacaat tggcgaaacc aacgccaact accgaaaatt attagagaac aatgatacca
     8341 taacctattt ttgcaaaacg ggaacgttta aagccgatga attggtggat attatttttg
     8401 agcaaaaagc aatcccttat tttgttaccc gaaaaatttt aaagtggttt ctctatgaca
     8461 aaccttcgga agaattggtg acgtattatg gagattattt tagaaaagtg aattttgaac
     8521 tgcaaccgtt gttgaccaag atttttacag aagaatacca aaagaatata tcgggaagca
     8581 aaatcaaaga tccattggtg tatatcctgc aactgaaata cgaattgaag atagaaaaaa
     8641 tgaatacacg agtattgacg tttttcttga aacaacaagg catggatttg tttaatcaac
     8701 caaatgtaaa aggttggaat ggtgggaatg aatggttgtc atcacaaatg tatttgcaac
     8761 gcaataatgt ggcagatttg ttgtgtagcg gacagccgtt aaaccgaaga agaaagtata
     8821 tgagcgatga aatgatggat gaaaatagtg atacgatgct tcgggtttcg atagattttg
     8881 atgcaaatgg caccaacaaa acaattattg cacaactgag cgaacgagtg ttgtttcaaa
     8941 cagatgactc gatgcagaaa gactttgaag agttgttgaa atatgatttt aatcccaaga
     9001 gcgaaggtgc agaaaacgct gtacttcgat tgtttaacac gatggtgaaa ttgcctgaat
     9061 tccaattgat atagttatga atagaagaaa ttttttatcc ttaacaggaa cttttacagg
     9121 aggttcatta ttgttgccaa gctttttaca tgcttttgga caacaaccta gtttggtgat
     9181 tggtgagcaa tgtttggtgt tcattcagct aaacggtggt aatgatggtt taaatacgtt
     9241 tgtgccttat gataatccgt tgtattatga tttgcgaagt aaaattgcgc tttctaaaga
     9301 tgttctttta ggcaaaaaca acggtatggg ttttcatcct gcgttaaaag attttgccaa
     9361 tatgcaacaa aatggtgatt tgagtgtgtt gcaaaacgtg ggttatcccg aaccagtgcg
     9421 ttcccacttt cgcagtcaag aaatatggca aacggctacc gatagtaata cttatgcaaa
     9481 caatggttgg cttgggaagt atttagattt gaagtgtaac gaccacgaac caacggcggg
     9541 aataaactta gacaatattg acaacttggc attgaaaggt gatgagccaa attcaatcac
     9601 agtgaaagac ccaaatagat ttaagattaa aaacgagaaa gaagaagtag gaaaactttc
     9661 ggataatccg cagttagact ttgttcggaa gattgccaac tcggtgatgg aaggttcgga
     9721 agacattcag aaagcgttgg caaaatcaac ggctgaggta agttatccga aaactaattt
     9781 gggtaaaaac ttagaatgga ttgcacgatt ggtaaaagga aacttgaatt cgaaagtgta
     9841 ttatacatcg caaaatggtt atgacacgca cgacaatcaa ttaaatattc agaatcgaaa
     9901 cttaacggaa ctgaacgatg ctgtggcgag tttttatagc gacttgaaga atgccaaact
     9961 gatgcaaaat gtaactgttg tagtgttttc cgaatttgga agaagagtaa aagacaacgg
    10021 cagtggcaca gaccatggaa cggcagctcc gatgtttgtt attggcggca gcaacaaagg
    10081 aaaagtattt gggaacaatc caaacctgtc tgaccttgat aatggcgatt taaaacatga
    10141 gattgatttc cgctccgtat atgctagctt gttgcacgac aaaatgaact ttgatgcccg
    10201 taagattggg ataaagaatg ctggttttaa agggttgttt tagattaata caacatctta
    10261 atttccttca atccaaaaga ctgtattgag tcatcttcca attgtatctg tagttttcca
    10321 tcggtagaaa catttttaat gatgcccata aacttttctt gagatgtgtt ttcaaacgcc
    10381 ataggttggt tgtgtttgaa taaatgctga tggtattgtt tccaaaagaa ttctttttga
    10441 gaatcgattt cttctaagtt ttgttgaata gtgttcgtta ttttgtcgag caatatttct
    10501 aggtcgtatt cgagaccagt tatttttttc aacgaactcg cttgaataaa atcaggaaaa
    10561 gattcttggt taacgttgat gccaattcca atgatggtat cgatagttgc atcggcttta
    10621 aggctgtttt cgataagtat gccaccaatc tttttgtttt ctgccaaaat gtcgttcggc
    10681 catttgatgt ttagttttgg aatttcaaat gtttctaatg cttcaataat cgaaacagca
    10741 atggttacat ttacgtcaaa aatagaagtg caaaattgtt tttttggttt gtataaaaga
    10801 ctaaaggtta agtttttacc aggttggcta gtccattgcg cacccatttg tccttttcct
    10861 ttggtttgag aaaccgtagt aactgtggtg aaattgtcaa gtggtttttc cttattcaat
    10921 tgcttcaaat aatcattagt agaatctgtg gcattgagtt tgattataag catggaacaa
    10981 ggtactttat tgttgctaat tttagacttt tattaagcaa aaataaacac aaaaagtaat
    11041 acctttgcaa ttcatagaaa aataatacat ggcgaaaaga gaagttcaat cagatatttt
    11101 attagcaaac ataataaaag gcatagaaga agtaaaagga aatgatattg acatcctaga
    11161 tttaagagaa atagacacag cagtttgtga ttattttatc atctgcaacg gaaattcaaa
    11221 cacccaagtt aacgctattg ttaactccat ccaaaaatta gtatccaaag aattaaaaga
    11281 caaaccatgg cacgtagaag gaacggacaa tggcgagtgg gttttgatgg attatgtgaa
    11341 cattgtggtt catgtgttcc aaaaacaaat ccgagaattt tacaatattg aaggtctttg
    11401 gggcgatgcc aaaataacta ccattccaac gaattactaa aaaataccta cacaaaaaat
    11461 ggctaacgaa aaaaaacaaa ataattttaa gttcagtccg tattggattt atggactttt
    11521 aatcggagtg ttcctattgt tgaatgtatt cggcggtgga agtttccaag aagtaggcaa
    11581 aataacttca tctaaattca acaccatgtt ggatagtgga aaagtagata aagttattgt
    11641 atacaataaa acagaagcag atgtttattt gaccgctgct gcaatgaaag aaaaagagaa
    11701 taaaaaagta gcttccgacg tgttgggaag agaaaataaa ggacctcatt atagttttga
    11761 aatagggaat gatgaattat ttcaaaagaa acttgaagaa gctaaaagtg agaacaaact
    11821 taaatctttt gatttcaaac aaagaggtga gtggactggg attttagtct ctattttacc
    11881 atttgttttc ataattggtt tgtggttttt tatcatgcga agaatgtctg gtggctctgg
    11941 tggcggaggc ggacaattat tcaatattgg aaaatcgaaa gcaaaactat ttgatgagaa
    12001 aacggatgta aaaattacat tcaaggatgt tgccggacta gaaggtgcaa aagaggaagt
    12061 acaagaaatc gtagaattcc ttagaaaccc agaaaaatac actaaacttg gcggtaaaat
    12121 tccaaaagga gctttattgg ttggacctcc aggaacgggt aaaaccttat tggccaaagc
    12181 cgttgcgggt gaagcaaaag taccgttctt ctctttatca ggttctgatt ttgtggaaat
    12241 gtttgttgga gtaggagcgt ctcgtgttag agatttattc aaacaagcca aagaaaaatc
    12301 gccagctatt atctttattg atgaaattga cgctgttggt cgtgctagag gaaaaaacaa
    12361 tatgtcgggt tctaacgatg agcgcgaaaa cactttaaat caattactga ccgagatgga
    12421 tggttttgga acgaacactc acgttatcgt tttggcagcc accaatagag cagacgtttt
    12481 agacaaagct ttgatgcgtg ctggtcgttt tgacagacaa atatatgttg acctgccaga
    12541 cgttagagaa cgtaaagaaa tatttgaagt gcatttggca ccattgaaaa aagtggataa
    12601 tttggatttg gattttttag ccaaacaaac accaggtttt tcgggtgctg atattgctaa
    12661 tgtttgtaac gaagcggcat tgattgctgc tagacaaaat aaagaagccg ttgataagca
    12721 agattttttg gatgccgttg accgaattgt tggtggattg gaaaagaaaa ataaaattgt
    12781 tactaaagac gaaaaacgtg ctattgcggt tcatgaagct ggacatgcta ctgtaagttg
    12841 gatgctagaa catgctgcac cattagtaaa agtaactatt gttcctcgtg gacaaagtct
    12901 tggagccgct tggtatttgc ccgaagaacg actaattgtt agaacggatc aaatgctaga
    12961 tgaaatgtgt gctactatgg gtggtcgtgc tgctgaaaaa gtagtgtttg ataaaatatc
    13021 tactggtgca ttgagtgatt tagaaaaagt aaccaaacaa gcacgtgcca tggttaccat
    13081 ctatggtttg aacgaaaaac tgggaaacat aacttattac gattcgtctg ggcaaagtga
    13141 gtatagtttt tcaaaaccct attcggaaga aacagccttg gtaattgata aagaaattaa
    13201 cacactgatt gaaaacgaat atcaaagagc aataaaacta cttgaagaaa ataaagataa
    13261 gttgctgaaa ctagccgata tactaattga aaaagaagtt atttttaaag acgatttaga
    13321 aactattttt gggaaacgtc cttttgataa gcatgaagac gaattagtag ccgacgctta
    13381 aaacacaaaa tttaacaatt ttataaaatc ttaattcaaa aactgttttt gaattaagat
    13441 ttttttatct ttgaacgatt cctaatagta agcttaagtt tatatatagc gcatgagcct
    13501 ttttagaaaa atatttggca gcagcaatga tgcctccgac gatgataacc agagcgaatt
    13561 taacaacatt ccttctagct tttcattgct tcctgttgat gaaaaattca cgtataattt
    13621 taagaaaaac ggtggaaagt tcatctattg cgaagatttg ggtgaagtaa aagaacaatt
    13681 tttaaacatc ttagaagaaa atgattggtt tgaatgtgaa gcaatgtgta ttgaaccaaa
    13741 attattttcg atgttggacg aaaacaaatt gcaatataag gatgttgaaa atcctaagtt
    13801 tctttttgca acatgtgaaa acttaatcgc cgaagaaggt tcggtattgt tttcttccaa
    13861 tcaaataaaa caacataaac caaacgattt acctgttaac attgttattt tggcaaaaac
    13921 aagccagatt atagaatcta aaagcgatgg actcagaggc ataaaaaaga attacgacaa
    13981 agattatcca aataacatca caaccataaa atattttgaa aaagccaaag acgaagattt
    14041 tcttcaatat ggaagtgttg caaaaaactt atatcttctg cttttagaag atttgtaaaa
    14101 tgaatgaaac cttaaaaaga gctatttctg gtgcagttta tattatttta ttattaagtt
    14161 gtataatata ttctaaagaa agtttagcaa tattattctc aatattatta gtctttggtg
    14221 tatatgaatt ttcaaaaatg acctctgtca atttttatgc a
//
LOCUS       DAOOBH010000050        14238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5514_length_14238_cov_6.988578, whole genome shotgun sequence.
ACCESSION   DAOOBH010000050 DAOOBH010000000
VERSION     DAOOBH010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14238
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5514_length_14238_cov_6.988578"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PK218_06590"
     CDS             complement(<1..212)
                     /locus_tag="PK218_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98208.1"
                     /translation="MPTNHQQEELWRKDAKNWKLGLFYHNKADQRIIVEKRNPAFGIT
                     FNFAHGKTYLYLLGMLLFFGFIVFVIT"
     gene            complement(215..955)
                     /gene="lptB"
                     /locus_tag="PK218_06595"
     CDS             complement(215..955)
                     /gene="lptB"
                     /locus_tag="PK218_06595"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPW98209.1"
                     /translation="MKLRAENLVKTYKKRSVVKGISVEVNQGEIVGLLGPNGAGKTTS
                     FYMIVGLVKPNSGNIYLDDMNITDFPMYKRAQYGIGYLAQEASVFRKLSIEDNILSVL
                     QLTKLSKEQQIAKMESLIDEFSLEHIRTNRGDLLSGGERRRTEIARALATDPKFILLD
                     EPFAGVDPVAVEDIQRIVAQLKNKNIGILITDHNVQETLAITDKTYLMFEGGILKAGV
                     PEELVEDEMVRRVYLGQNFELRKKKLEF"
     gene            complement(1095..1445)
                     /locus_tag="PK218_06600"
     CDS             complement(1095..1445)
                     /locus_tag="PK218_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPW98210.1"
                     /translation="MVDLVKEFNDYRSKMNDKLLADNNLVIKRIFNLDTNAYAEGALD
                     VKTKELLGLVASAVLRCDDCIKYHLETAYKNGVSKTEMMETMGIATLVGGTIVIPHLR
                     RAYEFWEALEENGQ"
     gene            complement(1438..2226)
                     /gene="tatC"
                     /locus_tag="PK218_06605"
     CDS             complement(1438..2226)
                     /gene="tatC"
                     /locus_tag="PK218_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPW98211.1"
                     /translation="MSFLDHLEDLRWLLVRSSAVIVFMAIVTYFFSDYIFDQIIFGPT
                     RTDFVTYRFFCDVAHRLGMTGDICVNELPFVIQNTSMEGQVNIFIWTCITAGFILAVP
                     YILYEVWKFISPALYHKEKQNASLFIVASSLLFFLGVLFGYFLIVPLSVNFVATFSIS
                     SVVKNEFNLESYIGMIKTSVIAAGLFFELPIIIYFLSKLGMVTPDFLRKYRKYAIIIV
                     LIVAAIVTPPDVVSQISVSIPMLIIYEASIFISVFVYKNKLKNG"
     gene            complement(2256..3221)
                     /locus_tag="PK218_06610"
     CDS             complement(2256..3221)
                     /locus_tag="PK218_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPW98212.1"
                     /translation="MIESKKILATAKKTILSESHAIAKLVDFLDENFVNAVEIIYKSK
                     GRLVVTGIGKSAIIAQKIVATLNSTGTPSLFLHASEAIHGDLGMVQRDDVVICISKSG
                     NSPEIKVLVPLLKRFGNKLIAITGNTTSFLGKESDFVLNTYVETEACPNNLAPTNSTT
                     AQLVIGDAIAVCLMEMKNFTAEDFAKYHPGGALGKKMLLRVGNMLETSHKPIVAPDSS
                     IKNVIVEISEKRLGVTAVVENEKVIGIITDGDIRRMLNKTENISGLTAKDIMSINPKT
                     IKSTDLVSDALNILEDFSITQLVVVDNNEYKGVLHLHDILKEGIV"
     gene            3308..5503
                     /gene="recQ"
                     /locus_tag="PK218_06615"
     CDS             3308..5503
                     /gene="recQ"
                     /locus_tag="PK218_06615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HPW98213.1"
                     /translation="MNSNEIDIHKELKKYFGFSQFKGLQEKVIKSIVQKENTFVIMPT
                     GGGKSLCYQLPALIEEGTAIVVSPLIALMKNQVDAIRSLSSEAGIAHVLNSSLTKTEI
                     NQVKKDITSGITKLLYVAPESLTKEEYVQFLKSVPISFVAIDEAHCISEWGHDFRPEY
                     RNLKTIIKHLGDVPVIGLTATATPKVQEDILKNLDMSDAKTFKASFNRPNLFYEVRTK
                     TKNIESDIIRFIKQHKGKSGIIYCLSRKKVEEIAGVLQVNGISAVPYHAGLDAKTRAK
                     HQDMFLMEDVDVVVATIAFGMGIDKPDVRFVIHHDIPKSLESYYQETGRAGRDGGEGH
                     CLAYYSYKDVEKLEKFMSGKPVAEQEIGFALLQEVVAYAETSMSRRKFLLHYFGEEFD
                     SETGEGADMDDNVRNPKTKVEAQNNVKKLLEVVRDTKHLYKSKEIVFTLIGRVNATIK
                     AHRTDAQSFFGCGVDQEERYWMALIRQVLVDGLLTKDIETYGILKISQKGLDFIKNPR
                     SFMMSEDHEYNEAEDDAIVTAAKSTGTADEVLMNMLRDLRKKVAKKLGVPPFVVFQDP
                     SLEDMALKYPISIEELFNIHGVGEGKAKKYGKEFIELIARYVNDNDIVRPDDLVVKST
                     GANSANKLYIIQNIDRKLSLEDIAKAKGMNMEALLKEMEQIVYSGTKLNISYWINEVL
                     DEDQQEEIHEYFMESESDNIENALKEFDGDYDIEELRLMRIKFISEVAN"
     gene            complement(5575..5814)
                     /locus_tag="PK218_06620"
     CDS             complement(5575..5814)
                     /locus_tag="PK218_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98214.1"
                     /translation="MSRKIKLIWDFRGESAAKIAEHHEKHLKEYIEIKKLEEKITGFE
                     VISELYAIAFMVVKEENMIQVRDALKPHRGEVYQK"
     gene            5869..6930
                     /locus_tag="PK218_06625"
     CDS             5869..6930
                     /locus_tag="PK218_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HPW98215.1"
                     /translation="MKIITSIFLLITTSCFSQELTLSQANHLATLPIKCLQQEYPNKL
                     SQLLIDSTEIQSPKVLHPAFYGCFDWHSSVHGHWSLVFLLNKFPNLENKEEIIKKLQI
                     NLSKENIQTEINYLNKTHEKSFERTYGWNWLLKLQLELETSNESFAKELAQNLKPLSN
                     LIIERYIEFLPKLLYPIRVGTHSNTAFGLTMAWDYAVYSKDENFQNSIKSNAKRLFNS
                     DKNCPMSWEPSGTDFLSPCLEEMAIMERILPKNEFLKWVKDFAPQLFLKNFNWEVAKV
                     SDRTDGHLVHLDGLNFSRAWNFYHLVNQYPNAFSHLKKVADYHFQFSLPSVVDGNYEG
                     EHWLASFALRTFEEKSKIK"
     gene            6979..8538
                     /locus_tag="PK218_06630"
     CDS             6979..8538
                     /locus_tag="PK218_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW98216.1"
                     /translation="MPREYQFQVSPEVAANENLLNQHVAKQFQISPKDIQKTVVLKRS
                     IDARQKAIKVNIKAKVYFVGEDFEENNFQLPEYKNVSNAQEVIIVGAGPAGLFAALQL
                     IELGLKPIVIERGKDVRGRRRDLKAINVEHIVNEDSNYCFGEGGAGTYSDGKLYTRSK
                     KRGDVDRILRLLVGFGATSEILVEAHPHIGTNKLPQIIQDIREKIIECGGKVLFETRV
                     TDFVISNNEIQGVVTQNDDIIPANKLILATGHSARDIFELLDRKKIVIEAKPFALGVR
                     AEHPQELIDKIQYSCDYRGEFLPPAPYSIVKQVNGRGMYSFCMCPGGVIAPCATSPGE
                     VVTNGWSPSKRDQVTANSGIVVELKLEDFKPFAKFGALAGMEFQKNIEQKAWQMAGKT
                     QKVPAQRLVDFSCQKISSDIPKTSYVPGTTSVELGDVFPNFLTQIMREGFQEFGKSMK
                     GYFTNEAILHAPESRTSSPVRIPRDDFSLEHPQIKGLYPCGEGAGFAGGIISAAIDGE
                     KCALKIAEVLK"
     gene            complement(8566..9675)
                     /locus_tag="PK218_06635"
     CDS             complement(8566..9675)
                     /locus_tag="PK218_06635"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HPW98217.1"
                     /translation="MKKIITIKHLILLSILFTLQACGQSTKKNANIIKDETMQNVIKK
                     PGNPYYSNTDTKKLNVSNEEWKKILPPDLYAVAREADTERAFTGTMWNDETKGTYYCA
                     VCGYKLFEAEQKFTSSCGWPSFFEQDNKESITFKADNSYGMVRTEALCGRCNSHLGHL
                     FDDGPAPTGKRYCMNAISLDFVPYKVATKTSNNTEKIVLGGGCFWCVEAVYENLDGVE
                     KVVSGYAGGNVSNPSYEEVSSGRTGAAEVIEITFDKSKTNLDEIFQVFFTVHDPTTLN
                     RQGADVGTQYRSVIFYTNEEEKKAAQSIISELNQKVFDGKIVTTIEKLDKFYPAESYH
                     QNYYEQNKSKGYCQMVIQPKIEKFEKVFKDRLKKK"
     gene            9833..10360
                     /gene="idi"
                     /locus_tag="PK218_06640"
     CDS             9833..10360
                     /gene="idi"
                     /locus_tag="PK218_06640"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HPW98218.1"
                     /translation="MKEEQVILVNEKDEPIGLMNKLEAHEKAVLHRAFSVFILNKDNE
                     LMLQQRAHQKYHSPLLWTNTCCSHQRDGETNIQAGTRRLFEEMGFKTNLKELFHFIYK
                     APFDNGLTEHELDHVMIGYYNDEPMINSDEVESWKWMKIEAVKEDMIQNPGLYTVWFK
                     IIFDKFYHYLEEHKL"
     gene            10464..10874
                     /locus_tag="PK218_06645"
     CDS             10464..10874
                     /locus_tag="PK218_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPW98219.1"
                     /translation="MKVTVSRKAHFNAAHRLYRKDWSMEKNDTVFGKCNNPNYHGHNY
                     ELIVSVTGEIDQETGFVIDVKILSDLIKEHIENAFDHKNLNLDVPDFKDLNPTAENIA
                     VVIWNKLRIFIDDKKDLEVTLFETPRNFVTYKGN"
     gene            10876..11328
                     /locus_tag="PK218_06650"
     CDS             10876..11328
                     /locus_tag="PK218_06650"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPW98220.1"
                     /translation="MALQIGDIVPNFTAKDNYGNDFDSKDYLGKKTLVIYFYPKNDTR
                     VCTEQACSFSDNYEDFKALGAEVIGVSSDTIDSHQQFSEKHKLPFILLSDSSKKLRKL
                     FGVPNDLLGLIPGRVTYVVDKNGMLLLVFNSMSGKIHIEKALEILKRM"
     gene            11338..12306
                     /locus_tag="PK218_06655"
     CDS             11338..12306
                     /locus_tag="PK218_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HPW98221.1"
                     /translation="MKNIFYPLQFLPILKERIWGGKKLNTFLNKPITSDFTGESWEIS
                     TVENDVSIIANGDFKGKTLNEIINEFPTEILGEKVVKQFGVQFPLLFKFLDAREDLSI
                     QVHPNDELAKKRHNSFGKTEMWYVMQADEDARLIVGFTEKSSQDEYLKNLKENTLLTI
                     LETKKVNQGDVFFLETGTVHAIGAGTIIAEIQQTSDITYRLYDFDRVDSNGNKRELHV
                     DLALDAINYNKIKAQKEYLKIENKSNEVVRCDYFTTHYIPLKGNYSVQRTKDSFCVYM
                     CVDGEFVLEFQNQKYNYRKGDTVLIPAEMNVYSFSGKASLLEIYIS"
     gene            12362..12628
                     /locus_tag="PK218_06660"
     CDS             12362..12628
                     /locus_tag="PK218_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98222.1"
                     /translation="MPSVKNLKKDINFVLGDIIEAVYIHELTTEGKPTEKTNAIIDEA
                     ITSFDALITKVNAKNVENKKAHFKQINVELEQVASQLVEKINAL"
     gene            12697..12770
                     /locus_tag="PK218_06665"
     tRNA            12697..12770
                     /locus_tag="PK218_06665"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12731..12733,aa:Thr,seq:tgt)
     gene            12877..13809
                     /locus_tag="PK218_06670"
     CDS             12877..13809
                     /locus_tag="PK218_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW98223.1"
                     /translation="MFGNKVLKGVLLVALGASSYGMLATFVKLAYSDVSSQGLHYTPA
                     EVIVAQFVIGIFVVLLLNFFQKNRKKNQVVKASATDIKKLMLVGTSTGLTSIFYYLAV
                     KYIPVSIGIVLLMQTVWMGVLLEAILEKKSPSKIKLFSVFIVLIGTLLATNVIANTVT
                     LDWRGIVLGLLAAASFTTTMFAANKVALSISSLQRSLYMLFGGIVVVFIFAFLTQKTP
                     FNFDIFYRWGILIALFGTIIPPILLNSGFPHTGIGLGSIVASLELPVSVTMAYFLLHE
                     EVLFIQWFGILLILTAIVIMNLNFKKNNISQLFS"
     gene            13839..>14238
                     /locus_tag="PK218_06675"
     CDS             13839..>14238
                     /locus_tag="PK218_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HPW98224.1"
                     /translation="MKKLFAEFFGTFWLVFGGCGSALFAAGFPDLGIGFAGVALAFGL
                     TVLTMVYAVGHISGGHFNPAVSFGLWAGGRFSAKELVSYIVSQCLGAICGAGTLFYIL
                     AGKEGFVIDNTKAGAFATNGYGTFSPDGYSM"
BASE COUNT         4848 a         2244 c         2403 g         4743 t
ORIGIN      
        1 gtaattacaa aaacgataaa tccaaaaaac aagagcattc caagtaaata gagatatgtt
       61 tttccgtgag caaaattaaa agtgattcca aaagcaggat ttcttttttc aacaatgatt
      121 ctttggtctg ctttattatg gtaaaacaat cctaatttcc aatttttagc atcttttctc
      181 caaagttctt cctgttggtg gttggttggc atttctaaaa ttccaatttc ttttttcgaa
      241 gttcaaagtt ttgaccaaga taaactcttc ttaccatttc atcttctacc aattcttctg
      301 gaacaccggc ttttagaatc ccaccttcaa acattaaata ggttttatcg gtgatagcaa
      361 gcgtttcctg aacattgtgg tcggtaatca ggatgccgat gtttttattt ttcaattgtg
      421 ctacaatacg ctgaatgtct tctacggcta ctgggtcaac tcctgcaaat ggttcgtcta
      481 gaagtataaa ttttggatcg gttgctaaag cacgcgcaat ttctgttcta cgacgttctc
      541 caccagaaag taaatcgcca cgattggtac gaatgtgttc taaactaaat tcatcaatca
      601 aactttccat ttttgcaatt tgttgttctt ttgaaagttt ggtcaattgc aatacactca
      661 atatattatc ttcaatactt aattttctaa aaactgatgc ttcttgtgcc aaatatccga
      721 ttccatattg tgcacgtttg tacattggaa aatcagtaat attcatgtca tctaagtaaa
      781 tatttccaga atttggtttt accaaaccta ctatcatata aaaagaagtt gtttttcctg
      841 caccatttgg accaagtaaa cctacaattt ctccttggtt tacttccacg gaaattcctt
      901 ttacaacact tctttttttg taggttttaa ctaaattttc ggctcttaat ttcattttag
      961 atataagatt ttaaatattc gtattacaaa atatggacaa agtaaaacat taacaacaaa
     1021 ctatttcaat caattaacaa atatttcgct gaaaagttaa ctaacctcaa acatactgtc
     1081 aactgaaaac ttttttactg accattttcc tccaaggctt cccaaaactc gtatgctctt
     1141 cttaaatgtg gaattacaat agttccgcca actaaagtgg caattcccat tgtttccatc
     1201 atttctgttt tagaaactcc atttttatat gcagtttcca aatgatactt tatgcaatca
     1261 tcacacctta aaactgctga tgctactaat cctaacaatt cttttgtttt cacatctaaa
     1321 gctccttctg cataagcatt agtgtcaaga ttaaaaattc ttttgattac taaattgttg
     1381 tctgctaata atttatcgtt cattttagac cgataatcat tgaactcttt tactaaatca
     1441 accatttttt aatttatttt tatacacaaa aactgaaatg aaaatacttg cttcataaat
     1501 aatcaacatc ggaatggata ctgaaatttg actaactaca tctggaggtg ttactattgc
     1561 tgccactatt aatacaatta taatagcata ttttctatat tttcttaaaa aatcgggggt
     1621 taccattcca agtttagaaa gaaaatagat gatgattggt aattcaaaaa ataatcctgc
     1681 tgcaattact gaagttttaa tcattccgat atatgattcc aagttgaatt cgttttttac
     1741 aacactactg atggaaaatg ttgctacaaa attcacagaa agcggtacaa ttaaaaaata
     1801 gccaaataaa actcctagaa agaaaagtaa tgaggaagcc actataaaaa gtgaggcatt
     1861 ttgtttttct ttatgataca atgctggact aataaatttc caaacttcat atagtatata
     1921 aggtacagca agaataaacc cagcagtaat acatgtccaa ataaaaatat tcacttgtcc
     1981 ttccatcgaa gtattttgaa taacaaatgg aagttcattg acacaaatat ctccagtcat
     2041 tcctaatctg tgagctacat cacaaaaaaa acgataagta acaaaatcag ttcttgtggg
     2101 tccaaaaatg atttggtcaa agatataatc gctaaaaaaa taagtaacta ttgccataaa
     2161 gacaattact gcagagcttc ttactaacaa ccatcttaaa tcttctaaat ggtctaagaa
     2221 agacatttca ttgacaggtt ttttattttt tgccattata caataccttc ttttaaaata
     2281 tcgtgcaaat gtagtacacc tttatattca ttgttatcaa ccacaaccaa ttgtgtaatc
     2341 gaaaaatctt caagaatgtt taaagcatcg ctaactaaat ctgttgattt tatagtttta
     2401 ggattaattg acataatgtc ttttgctgtt aatcctgaaa tattttctgt cttattcaac
     2461 attcttctaa tgtcaccatc tgtaattatt ccaataactt tttcattttc aactacagca
     2521 gtaacaccaa gtcttttttc agaaatttca acaattacat tttttatact agaatcaggt
     2581 gcaacaattg gtttatgact agtttccaac atatttccaa ctctaagcaa catttttttg
     2641 cctaaagcac cacctggatg gtattttgca aaatcttctg ccgtaaaatt tttcatttcc
     2701 atcaaacaaa cagcaatagc atcaccaata actaattgag cagtagtgct attagtaggt
     2761 gctaaattat taggacaagc ttctgtttca acataagtat tcaatacaaa atccgattct
     2821 ttacctaaaa aagaagtagt atttcctgta atcgctatta atttgttgcc aaaacgtttt
     2881 aataaaggaa ctaaaacttt tatttcggga ctattaccac tttttgagat acaaatcaca
     2941 acatcatctc gttgtaccat tcctaaatcg ccgtgaatag cttctgaagc atgaagaaaa
     3001 agagaaggtg ttccagtgga attgagagtt gcaacaattt tttgagcaat aatggcactt
     3061 tttccaatac cagtaacgac tagtcttcct ttagatttat agatgatttc tactgcatta
     3121 acaaaatttt cgtctaaaaa atcaactagt tttgcgatag catgactctc tgaaagtatt
     3181 gtttttttgg cagttgcgag aatttttttt gattcaatca attttgtgtt atttttatgt
     3241 ttgtatgtat aaaagattat gttatcttta tggatgcaaa tttaaataaa taactagtaa
     3301 gataagaatg aattctaacg aaattgacat tcataaagaa ttaaaaaaat attttggttt
     3361 ttcacagttt aaaggactgc aagaaaaagt tataaaaagt attgttcaaa aagaaaatac
     3421 ctttgttata atgccaactg gcggaggaaa atcactttgt tatcaattgc cagcattaat
     3481 tgaagaagga acagcaattg ttgtttctcc tcttattgct ttgatgaaaa accaagtgga
     3541 tgccatcaga agtttgtctt ctgaagcagg aattgcacat gttttaaatt catcattgac
     3601 gaaaaccgaa ataaatcaag taaaaaaaga cattacttct ggaattacaa aattgttata
     3661 cgtagctcct gaatcgttaa caaaagaaga atatgttcaa tttttaaaat ctgttccaat
     3721 ttcttttgtt gccattgatg aagcacattg tatttctgaa tggggacatg attttaggcc
     3781 agagtataga aatttgaaaa ccataattaa gcatttaggc gatgttccag ttattggatt
     3841 aactgcaacc gctacaccaa aagtacaaga agatattttg aaaaatttgg atatgagtga
     3901 tgcaaaaact tttaaagcat ctttcaatcg tccgaattta ttttatgaag tgcgtacaaa
     3961 aactaaaaat attgaatccg atattatacg ttttatcaaa caacacaaag gaaaatctgg
     4021 aattatatac tgtttaagtc gtaaaaaagt agaagaaatt gcaggagttt tacaagtaaa
     4081 tggaatttct gcggtacctt atcatgcagg attggatgct aaaactagag cgaaacacca
     4141 agacatgttt ttgatggaag atgttgacgt tgtagttgca accattgctt ttggaatggg
     4201 aattgacaaa ccagacgtac gttttgtaat tcatcatgat attccaaaat ctttagaaag
     4261 ttattatcaa gaaactggtc gtgctggtcg agatggaggc gaaggtcatt gtttggcata
     4321 ctactcctat aaagacgtag aaaaattaga aaaattcatg tcgggaaaac cagtagctga
     4381 gcaagaaatt gggtttgcat tattgcaaga agtagttgct tatgcagaaa cttccatgtc
     4441 gagacgtaaa tttctattgc attattttgg tgaagaattt gatagtgaaa ctggtgaagg
     4501 tgccgatatg gatgataatg ttcgtaatcc aaaaacgaaa gttgaagctc aaaacaatgt
     4561 taaaaaacta ttagaagttg ttcgtgacac caaacattta tacaaatcaa aagaaattgt
     4621 tttcacatta attggtcgtg taaatgccac tataaaagcg cacagaactg atgctcaaag
     4681 tttctttggt tgcggagttg atcaggaaga acgctattgg atggcattaa ttcgtcaagt
     4741 tttagtggat ggtctattga ctaaagatat tgaaacctat ggaattctta aaatttcaca
     4801 aaaaggattg gattttataa aaaacccaag atctttcatg atgtctgaag accatgaata
     4861 caacgaagca gaagatgatg caatagtaac agcagcaaaa tcaacaggaa cagcagatga
     4921 agtgctgatg aatatgcttc gtgatttgcg caaaaaagtt gcgaaaaagt taggtgttcc
     4981 accatttgta gtttttcaag atccttcttt agaagatatg gctctaaagt atccaatatc
     5041 aattgaagaa ttattcaata ttcacggtgt tggagaaggc aaagctaaaa aatatggaaa
     5101 agaatttata gaacttatag ctcgttatgt aaacgacaac gatattgtta gacctgacga
     5161 tttagtagtg aaatctactg gagcaaattc tgccaataaa ttatatatca ttcaaaatat
     5221 tgatagaaaa ttatcattag aagacattgc caaagctaaa ggaatgaaca tggaagcttt
     5281 gcttaaagaa atggagcaaa tagtatattc tggaacaaaa ttaaacatca gttattggat
     5341 aaacgaggtt cttgacgaag atcaacaaga ggaaattcat gagtatttca tggaatccga
     5401 atcggataat attgaaaacg ccttaaaaga atttgatggc gattatgata ttgaggaact
     5461 tcgattaatg cgaataaaat tcattagcga agtagcaaat taaaagaaat tccaaaaaat
     5521 aaattccaaa ttccaatcat tatctattgg aatttggaat ttttttattg ttttttattt
     5581 ctgataaact tctcctctgt gtggtttcaa agcatcacga acttgaatca tgttttcttc
     5641 tttaactacc ataaaagcta ttgcatacaa ttctgaaata acttcaaaac cagtaatttt
     5701 ttcttcaagt tttttaattt cgatgtattc ttttaaatgc ttttcatgat gctcggcaat
     5761 ttttgcagca gattcaccac gaaaatccca aatcaatttt atttttcttg acatattttt
     5821 gatttatcat acaaagataa gttttcgtaa atttgtgaaa accaaattat gaaaataatt
     5881 acttcaattt ttctgttaat tacaacttcc tgtttttcac aagaattaac actttcacaa
     5941 gccaaccatc tagcaacttt acccatcaag tgtttgcaac aagaatatcc caataaacta
     6001 agtcaactgc tcatagacag caccgagata cagtcgccaa aagtgttgca tcccgctttc
     6061 tatggttgct tcgactggca ttcctcggtt cacggtcatt ggagtttggt ttttttattg
     6121 aataaatttc caaatcttga aaacaaagaa gaaatcatca agaaacttca aatcaatcta
     6181 tccaaagaaa acattcaaac ggaaattaat tatctcaaca aaacacacga aaaatctttt
     6241 gaacgaacct atggctggaa ttggttattg aaattgcaac tcgaattgga aacttcaaac
     6301 gaatcttttg caaaagaact agcccaaaat ttaaaaccat tatccaattt aatcatcgaa
     6361 agatacattg agtttttacc caaattattg tatccaattc gcgttggaac tcattcaaat
     6421 actgctttcg gattaacgat ggcttgggat tatgcggtct attctaaaga tgaaaacttc
     6481 caaaatagta taaagtccaa tgccaaacga ttatttaatt ccgataaaaa ttgtcctatg
     6541 agttgggaac caagtggcac cgattttctt tcgccttgtt tagaagaaat ggctattatg
     6601 gaacgcatat tgcctaaaaa tgagtttcta aaatgggtaa aagattttgc accacaatta
     6661 tttttgaaaa atttcaattg ggaagttgcc aaagtttcag atagaaccga cggtcattta
     6721 gtgcatcttg acggtttgaa tttcagtcgt gcatggaatt tttatcattt agtaaatcaa
     6781 tatccaaatg cgttttcaca tcttaaaaaa gtagccgatt atcatttcca attttcatta
     6841 ccatccgttg ttgatggaaa ttatgaaggc gaacattggt tagcttcttt tgctttaaga
     6901 acatttgaag aaaaaagtaa aatcaaataa ctatttttgt tcgctgaaaa agatttctat
     6961 aatccgaaaa ataaaaaaat gccgcgcgaa taccagtttc aagtttctcc cgaagttgca
     7021 gccaatgaaa atttgctcaa ccaacacgtg gcaaaacaat ttcaaatttc tccaaaagat
     7081 attcagaaaa ccgttgtttt aaaacgctca atcgatgcgc gtcaaaaagc aataaaagtt
     7141 aacatcaaag ctaaagtata ttttgttgga gaagattttg aagaaaataa ttttcaatta
     7201 cccgaatata aaaacgtatc caatgcgcaa gaagtaatca ttgttggtgc aggaccagcc
     7261 ggcctttttg cagcgttgca attgattgag ttaggattaa aaccaatagt catcgaacgt
     7321 ggcaaagatg ttcgtggtcg tcgtagagat ttgaaagcaa taaatgttga gcatattgtc
     7381 aacgaagatt ctaattattg ttttggtgaa ggcggtgcag gaacttattc tgatggaaaa
     7441 ctctatacac gttcaaaaaa acgtggtgat gtagatagga ttttaagatt attagtcggt
     7501 tttggagcaa cttcggaaat tttggtagaa gcgcatccgc atattggaac caataaatta
     7561 ccacaaatca ttcaagatat tcgagagaaa atcatcgaat gtggcggcaa agtgttgttt
     7621 gaaactcgtg tcaccgattt tgtgatttca aataacgaaa ttcaaggcgt agtaacccaa
     7681 aatgacgata taataccagc caataaatta attttagcta caggtcattc ggcacgagat
     7741 atttttgaat tattagaccg aaaaaaaata gtaatagaag ccaaaccttt cgcgctaggc
     7801 gttcgagcag aacatccaca agagttgatt gacaaaattc aatacagttg tgattatcga
     7861 ggcgaatttc ttccgcctgc gccgtattcg attgtaaaac aggtcaatgg tcgtggtatg
     7921 tattcgtttt gcatgtgtcc tggcggcgtg attgctcctt gcgctacaag tcctggtgaa
     7981 gtagttacca acggctggtc gccatcaaag cgtgatcaag ttactgccaa ttcgggaatt
     8041 gtagtcgaat taaaattgga agattttaaa ccatttgcca aatttggcgc actagctgga
     8101 atggaatttc agaaaaatat tgaacaaaaa gcatggcaaa tggctggcaa aactcaaaaa
     8161 gtaccggcac aacgattagt tgatttttca tgccagaaaa tttcttcaga tataccgaaa
     8221 acctcttatg ttccgggaac tacttcggta gaattaggag atgtatttcc taatttttta
     8281 acccaaatta tgcgtgaagg tttccaagaa tttggcaaaa gcatgaaagg ttattttact
     8341 aacgaagcta tattgcatgc acccgaaagc agaacatctt ctcccgttag aattccaaga
     8401 gatgattttt ctttggaaca tccacaaatc aaaggattgt atccatgtgg cgaaggtgct
     8461 ggttttgctg gaggaattat atcagcagcc atcgatggcg aaaaatgtgc tttgaaaatt
     8521 gctgaggttt taaaataaaa tgtcccaatt tcttgggaca tcatactatt tctttttcaa
     8581 tcgatcttta aatacttttt caaacttttc aattttcggt tgaatcacca tttggcaata
     8641 acctttgctt ttgttttgtt cataataatt ttgatgataa ctctccgcag ggtaaaactt
     8701 gtctaacttt tctatagtag taacaatctt gccgtcaaaa accttttgat ttaattctga
     8761 aattatgctt tgggctgctt ttttctcttc ctcattagta tagaaaatta ctgagcggta
     8821 ttgtgtgcca acatcagcac cttgtcggtt taatgttgtt gggtcgtgaa cggtaaaaaa
     8881 aacttgaaat atttcatcca aattggtttt agatttgtcg aaggtaattt caattacttc
     8941 tgcagcacct gttcttcctg atgaaacttc ttcataactt ggattggaaa cgtttccacc
     9001 ggcatacccc gaaaccactt tttcaacacc gtctaaattt tcataaaccg cctctacaca
     9061 ccaaaaacaa cctcctccta aaacaatttt ttcggtattg ttggaagttt ttgtggctac
     9121 tttataagga acaaaatcta atgaaatagc attcatgcaa tagcgttttc cagttggtgc
     9181 tggtccatcg tcaaacaaat gtcctaaatg actgttgcat ctaccacaaa gtgcttcggt
     9241 tctaaccatt ccatagctgt tgtctgcttt gaaagtgata ctttctttgt tatcttgttc
     9301 aaaaaaactt ggccaaccgc agctacttgt aaatttttgt tcggcttcaa ataatttata
     9361 accgcaaaca gcacagtagt aagttccttt agtttcgtca ttccacatgg ttccggtaaa
     9421 agctctttca gtatcagctt ctctagctac agcgtacaaa tcgggaggca gaattttttt
     9481 ccattcttca ttcgatacgt ttaatttttt tgtgtcagta ttagaataat agggatttcc
     9541 aggttttttg attacgtttt gcatagtttc atcctttata atgtttgcgt tttttttagt
     9601 agattgtcca caggcttgaa gcgtgaatag aatcgatagt aaaattaagt gttttatggt
     9661 gataatcttt ttcataatta attttttgag ttacaaagtt actattgata tacgtgaata
     9721 attgttagat agtttacaaa tatggtggtt ttaaggaaag tttaacggtt gttctagcgg
     9781 tataattatc agcacttgat ttttttcgta tttttgagca tcattaaaaa tcatgaaaga
     9841 ggaacaagta attttagtca acgaaaaaga tgaaccaatt gggttgatga ataaactaga
     9901 agcgcatgaa aaagcggttt tacacagagc gttttcagtt tttattctaa ataaagataa
     9961 cgaattgatg ttgcaacaac gcgcacatca aaaataccat tcgccattat tgtggacaaa
    10021 tacttgttgt agtcatcagc gcgatggtga aaccaatatt caagcaggaa caagacgatt
    10081 atttgaagaa atgggtttca aaacgaacct taaagaattg tttcatttta tctataaagc
    10141 accgtttgat aatggattaa cagaacatga gttggaccac gtaatgattg gttattacaa
    10201 tgacgaaccg atgattaatt cagatgaagt tgaaagttgg aaatggatga aaattgaagc
    10261 agtcaaagaa gatatgattc aaaatccagg cttatatact gtctggttca agattatttt
    10321 tgataaattc tatcactatc tagaagagca taaattgtaa ataaatcaaa aagtaaaaaa
    10381 tataattgtg attaaattga agatagcttt aaactctttt aatcctttaa gaacaaaaac
    10441 ttttaatcca gaaatagaaa aaaatgaaag taactgtatc tagaaaagca catttcaatg
    10501 ctgcacaccg cttgtatcgt aaagattggt cgatggaaaa aaatgataca gtttttggaa
    10561 aatgtaacaa tcccaattat catggtcata actatgagct tatcgtttct gttactggag
    10621 aaattgatca agaaacaggt tttgttattg atgtaaaaat cctttccgac ttgataaaag
    10681 agcatattga aaatgctttt gatcacaaaa acttaaattt agacgttcct gattttaaag
    10741 atttaaatcc aacggctgaa aatattgcag ttgttatttg gaacaaactc agaattttta
    10801 ttgatgataa aaaagattta gaagttacgc tttttgaaac tccaagaaat tttgtaacct
    10861 ataaaggaaa ttaaaatggc tttacaaatt ggcgatattg ttccaaattt tactgcaaaa
    10921 gataattatg gaaatgattt tgatagcaaa gactatttag gaaaaaaaac tctagttatt
    10981 tatttttatc caaagaatga tactcgtgtt tgcaccgaac aagcttgcag ttttagcgat
    11041 aattacgaag attttaaagc acttggcgca gaagtaatag gagtaagtag cgatacgatt
    11101 gattcccatc aacaattttc tgaaaagcat aaattacctt ttattttact ctccgattct
    11161 tccaaaaaat tacgcaaact ttttggagtt cccaatgatt tattagggct aattccagga
    11221 agagttactt acgttgtgga caaaaacgga atgttgctat tggttttcaa tagtatgtcg
    11281 ggtaaaattc acatcgaaaa agcactcgaa attctcaaaa gaatgtaagt ttgcattatg
    11341 aagaatattt tttacccatt gcaattttta cccattttga aagaacgaat ttggggcgga
    11401 aaaaaactaa atactttttt aaacaaaccc ataacatctg attttacagg cgaaagttgg
    11461 gaaatttcta cagtagaaaa tgatgtaagt atcattgcta acggcgattt taagggaaaa
    11521 actttaaatg aaatcataaa cgagtttcca actgaaatat taggcgaaaa agtcgtaaaa
    11581 caatttgggg ttcagtttcc attgttgttt aaatttttag atgcacgtga agatttgtcc
    11641 atacaagtgc atccaaatga cgaattagca aagaaaagac acaattcttt tggcaaaacc
    11701 gaaatgtggt atgtgatgca agccgatgaa gatgcaagat tgatagtagg tttcacagaa
    11761 aaatcctctc aagacgaata cttaaaaaat ctaaaagaaa acactttgct cactattttg
    11821 gaaacaaaaa aagtaaatca aggtgatgtt ttctttttag aaaccggaac cgtacatgct
    11881 attggcgcag gaacaattat tgcagaaatt caacaaacct ctgatattac ctatcgtcta
    11941 tatgattttg atagggtaga ttcaaatgga aataaacgcg aattacatgt tgatttagct
    12001 ttggatgcaa ttaattataa taaaattaaa gcccaaaagg aatatttaaa aatcgaaaac
    12061 aaatcgaatg aagtcgtgcg atgcgattat tttactactc attatattcc tcttaaagga
    12121 aattattctg tacaaagaac caaagattct ttctgcgtat atatgtgtgt agatggcgaa
    12181 tttgtgttag agtttcagaa tcaaaaatac aattacagaa aaggcgatac ggtacttatt
    12241 ccagcagaaa tgaatgttta ttctttttct ggtaaagctt cattactaga aatttatatt
    12301 tcttagtttg ttttcaaaag aattagtgta attttgcacc aaattttaaa aataagagaa
    12361 aatgccaagt gtaaagaatt taaaaaaaga tattaatttc gttttaggag atattattga
    12421 agcagtttat atccacgaat tgactactga agggaaacca acagaaaaaa cgaatgctat
    12481 tattgatgaa gctattactt catttgatgc tttgattact aaagtaaatg caaaaaatgt
    12541 tgagaataaa aaagctcatt ttaagcaaat aaatgtagaa ttagaacaag ttgctagtca
    12601 attggttgaa aaaataaacg cactgtaaag aaaaaaaagt gtaaaaaagt tttggatttt
    12661 aaatttctaa aaatatattt gcactcgaat tgagatgcca gcatagctca gttggctaga
    12721 gcagctgatt tgtaatcagc aggtcgtggg ttcgagtccc tctgccggct caattgtaaa
    12781 accatcacag aaatgtgatg gtttttttta ttaatttatt tttctaaaaa gaactttaga
    12841 attggttatc tttgtcggct ttttaaaaaa ataaaaatgt ttggaaataa ggtattaaaa
    12901 ggagttttat tggtagcatt aggagcttca agctacggta tgcttgccac ttttgtaaaa
    12961 ttggcttact cagatgtaag ttcacaagga ttgcattata cgccagcaga agttattgtg
    13021 gcacaatttg taattggaat atttgtagta ctattattaa acttttttca aaaaaataga
    13081 aaaaaaaatc aggttgttaa agctagtgcg actgatatta aaaaactcat gttggtaggt
    13141 acttcaacag gtttgactag tattttttat tatttagcgg taaaatatat tcctgtttcc
    13201 attggaattg tattgctaat gcaaacagtt tggatgggcg ttttgcttga agcaatttta
    13261 gaaaaaaaga gtccatcgaa aattaaatta ttttcggtat ttattgtttt aattggaaca
    13321 ttgcttgcca caaatgtgat tgctaatact gttactttag actggcgagg cattgtttta
    13381 ggattgcttg cagcagcttc ttttaccaca acaatgtttg ccgctaataa agtggcactt
    13441 tcaatttctt cgttgcagcg cagtttatat atgctttttg ggggtattgt tgtcgttttt
    13501 atttttgctt ttttaactca aaaaacacca ttcaattttg atattttcta cagatgggga
    13561 attttaattg cactttttgg taccataatt ccgccaattt tgttgaattc aggttttccg
    13621 catactggaa ttggattagg aagtattgtt gcctctcttg aattgccagt ttctgtaaca
    13681 atggcatatt ttttattgca cgaagaagtg ttgttcatac aatggtttgg aattttgttg
    13741 atacttacag ctattgtgat tatgaactta aattttaaaa aaaataacat ttctcaatta
    13801 ttttcataaa tttgataatc aactattaat caataattat gaaaaaacta ttcgcagaat
    13861 tttttggaac cttttggctt gtttttggag gttgtggtag tgcacttttt gctgctggat
    13921 ttcccgattt aggcattgga tttgcaggtg ttgctttggc atttggactt accgttttga
    13981 caatggttta tgctgttggt cacatttcag gcggtcattt taatcccgca gtttctttcg
    14041 ggttatgggc tggaggaaga ttttcggcta aagagttagt ttcttacatt gtttctcaat
    14101 gtcttggagc aatttgtggc gcaggaacat tattttatat acttgcagga aaagaaggtt
    14161 ttgtaatcga caatacgaag gctggtgcat ttgccactaa cggttatggc acattttctc
    14221 ctgatggtta ttcaatgt
//
LOCUS       DAOOBH010000051        14182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5550_length_14182_cov_5.465633, whole genome shotgun sequence.
ACCESSION   DAOOBH010000051 DAOOBH010000000
VERSION     DAOOBH010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14182
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5550_length_14182_cov_5.465633"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..4003
                     /locus_tag="PK218_06680"
     CDS             179..4003
                     /locus_tag="PK218_06680"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HPW98225.1"
                     /translation="MKKLLFLLAFLLSIASGYAQVGINTTTPDAQLDIKSSNQATPTN
                     TDGILIPKIDAFPVTNPTAAQNSMMVYLTTTSAGKSPGFYYWDNATTSWKGFGNGSGW
                     SLTGNTGTTPGTNFVGTTDDKDLIFKRNNTLTGKLTPNNVAFGVGALQLNTTGSNNVA
                     NGYSSLNFNTTGTDNVAIGPFSSYKNTTGSYNVSNGSYSLYNNINGSENVGIGHASLN
                     ANSSGINNVATGSWAATSNIDGNNNTAIGMESLAANKSGSSATAIGYQAMRYANNTTT
                     TFDNKNVAVGYRSLFGSTNPVNNTGNNNTAIGYQSLLLNTSGNSNAALGNDTLNKNTT
                     GSNNTANGDNALFANTTGSNNTALGNNTLNGNTTGSNNIAIGSNANVPITNNNDQLSI
                     ANVIYGITMNSTANGKIGIGTPTPTEKLEVAGKTKTTNFQMTNGASAGRVLVSDATGN
                     GVWTAPGVATGGWALGGNVGTNESINFIGTTDYQSLIFKVNNAQYGKLSRTGGSVFFG
                     EDAGLSNGGIYNIGIGYGTLRVSNPGRENVAIGGSSMYNNVGGTMNVAVGTASLNLNT
                     LGNFNTALGGNSLKLNTTGISNVATGHNALFNNTIGSANVSIGNETLLSNNAGSKATA
                     IGFGAMYYANDTSTYFENKNVAVGYYAMYGSTTAANNTGNNNTAIGYQTLLNNTSGNN
                     NVALGTDTLGANTTGSNNTGLGNNTLAGNTTGTNNIAIGSNANVPTVNGNDQLSIANV
                     IYGITMNSTSNGKIGIGTPTPTEKLHVIDNIKVGRSQWFSTANDRFIKFGDGNYATIG
                     EEIADDYISLKAKNFLFNPSISGDPVGYPGNVGIGILTAPTEKLEVAGKTKTTNFQMT
                     TGAALGSILTSDASGNATWQTPTPASDDWTITGNAGTNPAINFLGTTDNNDVVFRRNN
                     IRSGLLNLASNNTSFGVAALKGTTVGTNNTAIGKDALSGNVIGNDNIAIGVETLSSQN
                     IFNSPAGNQNIAIGNYALKNLGINSYGADGMIAIGYEAMSLVTGSVNHAIAIGTRAMQ
                     YEGSPATDDTLNRNIALGYEALRGSTNVNNNNGNSNTAIGFQTLTVNTSGYNNSALGD
                     SSLIANTTGANNTALGKDAGKTITTGNNNIVIGYDAQVSSATTNNQIRLGNTAITYAG
                     IQVPWTITSDRRYKSNIQTSNLGLDFIKKLNPVSYIRKNDDSKKTEYGFIAQEVEQML
                     NELNTINSGIISKDDAGMYSVRYNDLLSPMVKAIQEQQQTIEGQQKIIEEQNKKIQSL
                     EERLKKIESKLW"
     gene            4081..5007
                     /locus_tag="PK218_06685"
     CDS             4081..5007
                     /locus_tag="PK218_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPW98226.1"
                     /translation="MKIEQQKEIISGFKHLELLANQVVEGFISGMHKSPFHGFSAEFA
                     EHKIYNSGESTKNIDWKLFAKTDRLYSKRYVEETNLRCHLIIDNSSSMYYPKLENDQP
                     FYKNKIGFSVLASAVLMNLLKKQRDAVGLSIFSDKYDYYAPEKGSDRHHRMLLNILEE
                     LLEKPKQTKTTDTITYLHQIAEKMHRRSMIVLFTDMFQNQDKEKLFNALQHLKHNKHK
                     VVVFHVYDKQTEIEFDFDNAPRKFIDVESGEELNLFAENIKGFYEEKIKLYFNEIAMT
                     CAQNKIKYIPVSVDESFEKILTTYLVEKQMFG"
     gene            5093..5166
                     /locus_tag="PK218_06690"
     tRNA            5093..5166
                     /locus_tag="PK218_06690"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5127..5129,aa:Asp,seq:gtc)
     gene            5429..5905
                     /locus_tag="PK218_06695"
     CDS             5429..5905
                     /locus_tag="PK218_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98227.1"
                     /translation="MKKTLALLVLFFTFSCQYFDRKVPSEDVLLKEQLKEINWSEIDE
                     YPTVVDCEKITDETQRKQCFFEFLTQTIQQKLDIDTLVNLLPKLDTIEVKVTVFPNSL
                     VTFEPQFPKDSVAYDTIKIDSILRSRLIDFPKIKPAIKRGIPVKTQFILPVILKIE"
     gene            complement(5910..7031)
                     /locus_tag="PK218_06700"
     CDS             complement(5910..7031)
                     /locus_tag="PK218_06700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW98228.1"
                     /translation="MEIIFFILIVVYAVFILQLFFGFNKIKDQSISKEYTPKTNFTIV
                     VPFRNEAKNLSKLLASLSNLNYPKNFIEIIMIDDDSKDNSSAVFIKWRMKNDEIDTTL
                     LENLRLSNSPKKDAISRAMPIAKNPWIITTDADCEVNPNWLSSIDGFIQKNSFEMIAA
                     PVIYKTKNNWFHHFQQFEMLSLQATTIGSFGINQPFMCNGANFAYTKKMFLELDGFED
                     NNKIASGDDVFLLQKAIAKCSDKVGFLKAQNAIVITKPENNLFKLLMHRIRWASKTSN
                     YNSFYPKFLASSVFFANAFIAFGLIALFFGKLDWKLFLILFLIKYSIDYLLILKANSF
                     FNTNKIAIPFASAILYPFFATTVGVFSLFGKFTWKGRRF"
     gene            complement(7022..7936)
                     /locus_tag="PK218_06705"
     CDS             complement(7022..7936)
                     /locus_tag="PK218_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98229.1"
                     /translation="MSKSKNKLVILILKISIVLLAFYFIYTQITKKNNFNWTDFVGII
                     QNKFSFGIISALLFLSFLNRYLEILKWKNLVSYIAPISIFEATKQVLAALTGGIITPN
                     GIGEYAGKVLFFEKSKSKIIIFLNLICNGIQMILTILFGLFGLIVLGYYEIAFTLFGI
                     SIVVLVIVFLTKNFKIKNYSIASVLQKTNEIPKKIHQRNMFLGLSRYVVFSHQYYLLF
                     VVLDVHLPYITLISTIAVVYFLASSLPSFQMFDFAVKGSVALFFFGKHDINEWIILFV
                     STLMWFLNVVLPVLIGSFFVLNFKSKWK"
     gene            8068..11328
                     /locus_tag="PK218_06710"
     CDS             8068..11328
                     /locus_tag="PK218_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HPW98230.1"
                     /translation="MKIKLHLLIVFLSFSAFSQNIIWEKVTSREITKNKFVERDNFPN
                     QFDLYQISTSVLKNTLLQSPNRLQSNSSKVIISIPNLNGELERFQMFEFSNFAPQLQA
                     QYPEIRSYVGQGIEDKTAVLRLSSDPRGFQGMILRANNESEFFEPYSTDGSVYAFYDS
                     ERKNGDLPFVCSTQDLALNNELKSATTNQRSNSGELLTFRLALSCNGEYAQYFGGSVA
                     QALAGMNATMTRVNGVFENDFAIHMNIISNNNLVVYTNPSTDPYSTNLGSWNGQLQNT
                     LTNIIGEANYDIGHMFGASGGGGAAGCIGCVCVDGSKGSGITSPGTGGPVGDTFDIDY
                     VAHEMGHQFGANHTFSHNVEGSGVNVEPGSGSTIMGYAGITSRDVQPHSDAYFVYSSI
                     KQIQDNMVEKHCPQRVTLSNIAPIVNGGLDYTIPKSTPFVLTGEASDTNGDILSYCWE
                     QNDTATTQTGANSGASPTKTNGPNFRSYSPVVSSSRYFPRIQSIVANQATTSGTEIIV
                     EALSSVSRTLNFVLTVRDNFAGAGQTNSDAMVVTVNAAAGPFVVTSPTTAVSWTVGTN
                     QNVTWNVAGTTTNDINAEYVDIFLSTDGGFTYPIQLASKVPNDGSELITVPNNIGTTN
                     RIMVKGYKHIFFDISNTNFSIIAPTSSFAVAYNGVAEQQNKEICRGSSVTYTIPYTAY
                     AGFSGTTLFAVSGEPDNSTITFSPSTASNSQDITMTVETTNNSTPGVYSMLVTGISGT
                     TTNSAPFYLNLFDANFSSLTLTSPADQSIGQQVDLNLTWNIDSNATLYDVQVSTESDF
                     SSIVYSGTVSTNSFALTNLASATRYYWRVLPKNNSCSGVYSGAYLFKTGQITCTDFSS
                     TNVPITIPTTANATVNSTLSVTNTDVISDANVTLNIAHTWVNDLTITLISPAGTEVQL
                     VGQPCTSDDFQNITATFDDSGIPVVCGANPAISGTVFPAQALSAFNGETMNGTWTLRV
                     LDSFNQDGGSINGWSLNLCSNTAVPLATTENSLQNFALYPNPNNGSFTVSFNSNSTNK
                     ISVDVFDIRGRNVFSNEYQNNGIFNENIQMSTIQSGIYLVKVQDGEQQLTKKIVIN"
     gene            complement(11448..13073)
                     /gene="pckA"
                     /locus_tag="PK218_06715"
     CDS             complement(11448..13073)
                     /gene="pckA"
                     /locus_tag="PK218_06715"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPW98231.1"
                     /translation="MENTAQFTKSISLDKYGIKNVQEIIYNPSFEFLYNEELSPNLKG
                     FEKGQLTELGAVNVMTGEFTGRSPKDKYIVKDAVTENTIWWNSDKAVNDNKPISQDTW
                     NALKETSVNQLSGKRLFVVDAFCGANENTRLKVRFIMEVAWQAHFVKNMFIRPTEEEL
                     ANFGEPDFVVINASKANFEDYKAYNLNSDVYIAFNLSEKIQIIGGTWYGGEMKKGMFA
                     MMNYYLPLQGIASMHCSANKGKDGDVAVFFGLSGTGKTTLSTDPKRELIGDDEHGWDN
                     EGVFNFEGGCYAKTIDLSKENEPDIYGAITKDALLENVTVNADGIIDFKDGSVTQNTR
                     VSYPINHIQNIVKPVSKAGHATKVIFLTADAFGVMPPVSKLTPEQTKYYFLSGFTAKL
                     AGTERGVTEPQPTFSACFGKAFLTLHPTKYGEELVKKMEANNATAYMVNTGWNGTGKR
                     ISIKDTRAIIDAILDGSIEKAPTKTIPVFNFEVPTQLNNVNSAILDPRDTYGDKNDWN
                     VKAEDLAYKFIKNFEQYTDNEKGQNLVKAGPQL"
     gene            complement(13154..13540)
                     /locus_tag="PK218_06720"
     CDS             complement(13154..13540)
                     /locus_tag="PK218_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HPW98232.1"
                     /translation="MDRRMAIVGSFLGLTAIILGAFGAHALKVRLSVEQLSSFETGVK
                     YQMYHALFLLVLSFNSHCTEKLKKTLFYLIVSGVLLFSGSIYLLSTRVISGFELKFLG
                     PITPIGGLLLIVAWTFLFMHFIKKKI"
     gene            13661..>14182
                     /locus_tag="PK218_06725"
     CDS             13661..>14182
                     /locus_tag="PK218_06725"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPW98233.1"
                     /translation="MKKIALFIVFAFILSSFTADSGYGIKSGEKLVYAGSYNMSGLLT
                     QIAQVTMSTENVTTSKSNYLHLSCELATYTKWDSFFKIRDIYESYVNPTTLKPSLYKR
                     SIDEGGYTIKEKYVFKGTNVKSSVTKKNRPEVHKSFTIGTNTQDVISLFYKVRTLDFS
                     KFKTGQTQSMMIVF"
BASE COUNT         5025 a         2371 c         2302 g         4484 t
ORIGIN      
        1 acaataatac agtttaaggc ttgcataaaa tgattttctc tttcaaatat aaatattttt
       61 ttctcataaa aagttgttag tgtatttaaa atgtcgttcg tccttttgtg ctttttaagt
      121 cgtttagaat gaatcaattt tgtagtgctt aaaatactaa ttacttaaat ctaaaaagat
      181 gaaaaaatta ctttttcttt tagcttttct actttctatc gcttctggat atgcccaagt
      241 aggaataaac acaactacac cagatgctca attagacatt aaatccagca accaagctac
      301 acctactaat actgatggaa ttcttattcc gaaaattgat gcctttccag taaccaatcc
      361 gacagcagca caaaacagta tgatggttta tttaaccaca acttcagcag gtaaatcccc
      421 tggattttat tattgggata atgctacgac cagttggaaa ggatttggga atggttcggg
      481 ttggagttta acaggtaaca caggaactac gccgggaaca aattttgttg gtacgactga
      541 tgataaagat ttgattttta aaagaaacaa tacgttaact ggaaaattaa cacctaacaa
      601 tgttgctttt ggagttgggg cattacaatt aaatacaaca ggaagtaaca atgtagctaa
      661 tggttattct tcgttaaatt ttaacacaac aggaactgat aatgtagcaa ttggtccttt
      721 ctcttcatac aagaatacta caggttctta taatgtgtca aatggatcat attctctata
      781 caataatata aatggttcag aaaatgttgg tattggtcat gcatctctta atgcaaattc
      841 atctggtatt aataatgttg caactggtag ttgggcagct acatcaaata ttgatggtaa
      901 caacaataca gctataggaa tggaatcttt agccgccaat aaatcgggtt cttctgctac
      961 tgcaattggt tatcaagcta tgagatatgc taataacaca acaactactt ttgataataa
     1021 aaatgttgca gtaggttatc gttcactatt tggatcaacc aatccagtta ataacactgg
     1081 aaataacaac acagcaatag gttaccaatc attacttcta aatacatctg ggaatagtaa
     1141 tgctgcatta gggaatgata cacttaataa aaacactact ggtagtaata atactgccaa
     1201 tggcgataat gcattatttg caaatacaac gggttcaaac aatactgctt taggaaataa
     1261 tactttaaat ggaaatacaa cgggttcaaa taatattgca attggttcaa atgcaaatgt
     1321 cccaataacg aataataacg accaattatc aattgctaat gtaatttatg gaataaccat
     1381 gaattcaaca gccaatggta aaatagggat aggaactcca actccaactg aaaaattaga
     1441 agtagctgga aaaaccaaaa ccactaattt ccaaatgaca aacggtgctt cggctggtag
     1501 agttttagtt agtgatgcta ctggtaatgg tgtttggaca gcaccaggag ttgccactgg
     1561 tggttgggca ttaggcggaa atgtaggaac aaacgaaagt attaacttta taggtacaac
     1621 cgattatcag tcattaattt ttaaagttaa taacgctcaa tatggtaaat tatctagaac
     1681 aggcggaagt gttttttttg gtgaagatgc aggattgtca aatggtggta tttataatat
     1741 aggaatcgga tatggaacat taagagttag taacccaggc cgtgaaaatg ttgcaatagg
     1801 aggatcatca atgtacaata atgttggtgg tactatgaat gttgcggtag gaacagcatc
     1861 attaaatttg aacactttag gaaattttaa tactgcatta ggtggaaatt ctttaaaact
     1921 aaatactacc ggaatttcaa atgttgctac aggacacaat gctttattca ataacacaat
     1981 aggatctgca aatgtatcaa ttggcaatga aactctttta agtaataatg ctggttcaaa
     2041 agctactgca atcgggtttg gtgctatgta ttatgctaat gatactagta cttactttga
     2101 aaacaagaac gttgctgttg ggtattatgc tatgtacggt tctactacag cagccaacaa
     2161 tacgggaaat aataatacag ccataggata ccaaacactt ttaaataata cttcaggcaa
     2221 taataatgta gctttaggaa cagatacctt aggagcaaac actacaggtt ccaacaacac
     2281 tggcttagga aacaatactt tagcaggaaa cactactggt accaacaaca tcgctatcgg
     2341 ttcaaatgca aatgttccaa ctgttaatgg caacgaccaa ctgtcaattg caaatgtaat
     2401 ttatggtatt acgatgaatt caacctccaa cggaaaaatt ggaatcggga caccaacacc
     2461 aactgaaaaa cttcatgtaa tagataacat aaaagtaggt agaagccaat ggttttcaac
     2521 agcaaatgat cgttttataa aatttggtga tggaaattat gcaaccattg gagaagaaat
     2581 tgcagatgat tatatatcat taaaagccaa aaattttcta tttaatccaa gtatctcagg
     2641 agatcccgtt ggttatccag gaaatgttgg tatcggtatt ttaactgcac ctaccgaaaa
     2701 acttgaagta gccggaaaaa caaaaaccac caatttccaa atgacaacag gtgctgcatt
     2761 aggtagtatt ttaacttcag atgcttcagg taatgccact tggcaaacgc caacaccagc
     2821 aagtgacgat tggactatta caggaaacgc aggaaccaac ccagcaatca attttttagg
     2881 gacaaccgat aataatgatg tagtatttag aagaaacaac atccgttctg gattattaaa
     2941 cttagcaagt aacaatactt cttttggagt tgctgcttta aaaggaacta ctgtaggaac
     3001 aaataacacg gcaataggga aagatgcttt atcaggtaat gtaattggca atgataacat
     3061 agctattggt gttgaaactt tatcaagtca aaatatattt aatagtccag ctggaaatca
     3121 aaacattgcg attggtaatt acgcgctaaa aaacctaggg attaatagtt atggtgcaga
     3181 tggaatgata gcaattggtt atgaagctat gtctttagta actggaagtg ttaaccatgc
     3241 tatagccatc gggacaagag ctatgcaata tgaagggtct cctgcaacag acgatacact
     3301 taatagaaat attgctctag gatacgaagc actacgtggt tctacaaacg ttaataacaa
     3361 taatggaaat agtaatacag caattggttt tcaaacctta acagttaata catctggcta
     3421 taataattca gctcttggag atagctcatt aattgcaaac actacaggtg ctaataatac
     3481 cgctttgggc aaagacgcag gaaaaaccat cacaacagga aacaacaata tcgtaattgg
     3541 ctacgatgcg caagtatcat cagcaactac taataaccaa atacgtttag gaaatactgc
     3601 cattacctat gcggggatac aagtgccatg gacaataact tccgacagaa gatacaaatc
     3661 aaatattcaa acttctaatc ttggtttaga ttttataaaa aaattaaatc cagtatccta
     3721 catcagaaaa aatgatgatt ctaaaaaaac agaatacggt tttatcgctc aagaagtaga
     3781 gcaaatgtta aatgaattaa atacaatcaa tagcggtatt atttcaaaag acgatgctgg
     3841 catgtatagt gttcgatata atgatttatt atcaccaatg gtaaaagcta tacaagaaca
     3901 acaacaaaca atagaaggac aacagaaaat catagaagaa caaaacaaaa aaatacaatc
     3961 actagaagaa cgattaaaaa aaatagaaag caagttgtgg taatttgagt ttttaagcag
     4021 aaattggtct aacgaaagtt aggccttttt tattcaaaaa tttcaaatta tatttgaatt
     4081 atgaagatag aacaacaaaa agaaatcatt tcaggattca agcatctcga attgttagcc
     4141 aatcaagtcg ttgaaggttt tatttcgggt atgcacaaat cgccatttca cggattttct
     4201 gccgaatttg ccgaacataa aatctacaat tcaggcgaaa gcactaaaaa tatcgactgg
     4261 aaactttttg caaaaacaga cagattatat tccaaaagat acgtagaaga aaccaacctg
     4321 cgttgccatt taatcatcga taattcgtct tcgatgtatt atccaaaact agaaaatgac
     4381 caaccattct ataaaaataa aataggtttt tcagtcctag cttcagcagt attaatgaat
     4441 ttgctcaaaa aacaacgcga tgcagtaggc ttaagtattt tttcagataa atacgactat
     4501 tatgcaccag aaaaaggatc cgatagacac cacagaatgt tattaaacat tttggaagag
     4561 ttgttagaaa aaccaaagca aaccaaaaca acagacacga ttacctattt gcatcaaatc
     4621 gccgagaaaa tgcaccgtcg ttccatgatt gttttgttta cagatatgtt tcaaaatcaa
     4681 gacaaagaaa aactctttaa tgccttgcaa catttgaaac acaataagca caaagtagtt
     4741 gtttttcatg tgtatgacaa acaaacagaa atagagtttg actttgacaa tgccccaaga
     4801 aaattcattg atgtagaatc tggcgaagag ttaaatcttt ttgccgagaa cattaaaggt
     4861 ttttatgaag agaaaatcaa gttatatttc aatgaaatcg ccatgacttg tgcccaaaat
     4921 aaaataaaat acattccagt atccgttgat gaaagttttg aaaaaatact aacaacatat
     4981 ttggttgaaa agcaaatgtt tggataaaaa atacgatttt taaaaatatt tttaagtaaa
     5041 aaagttgcag attacaaaaa cgattgtata tttgcaaccg caattaagcg atggtttggt
     5101 agttcagttg gttagaatac atgcctgtca cgcatggggt cgcgggttcg agtcccgtcc
     5161 agaccgcaaa attgggaaaa ggttttcaga aatgaaaacc ttttttgccc agtaaaagta
     5221 ttgaaaatta gttgtgttgt ttcgctacga atttgtaact cgtagctcgg tttagtagtt
     5281 agaccaatga aaagcttttc gcctcggcga atggcttttt tgctttaaag acctttcgat
     5341 tttataattc aaacaatgtt tcttctaaaa atcagattaa agctcaacta ttttactatt
     5401 tttgaaattc taatgtattt ttgaattcat gaaaaaaacg ttggctttat tagtattatt
     5461 tttcactttc tcttgtcaat attttgacag aaaagtgcct tccgaagatg ttttgttaaa
     5521 agagcaactc aaagaaatca attggagtga aatagacgaa tatccaacgg ttgttgattg
     5581 tgaaaaaatt actgatgaaa cgcaacgcaa acagtgtttt tttgaatttt tgacacaaac
     5641 catccagcaa aaactagaca tagacacgtt agtaaactta ttgccaaaac ttgatactat
     5701 tgaagtgaaa gttaccgttt ttcctaactc attggtgact tttgaaccac aatttcctaa
     5761 agactctgta gcttatgata ccataaaaat tgacagcatt ctgcgttcaa gattaattga
     5821 ttttccgaaa attaaacccg ccattaaaag aggaattcct gtgaaaacac aatttattct
     5881 tccggttatt ttaaaaatag aataacaact taaaaacgcc ttcctttcca agtaaacttt
     5941 ccaaacaagc taaaaacacc aacagtagtt gcaaaaaacg gatagagaat agcactggca
     6001 aatggaatag ctattttgtt agtgttaaaa aatgaattcg cctttaaaat cagaagataa
     6061 tcaatactat attttatcaa aaatagaatt aaaaacaact tccaatctaa ctttccaaaa
     6121 aataaagcaa ttaatccaaa agcgataaaa gcatttgcga agaaaacact acttgccaaa
     6181 aattttggat agaaactatt gtaattagaa gttttgctcg cccagcgaat gcgatgcatc
     6241 aataatttaa acaaattatt ttctggtttt gtgattacaa tagcattttg cgctttaaga
     6301 aatccaactt tatcagagca ttttgcaatt gctttttgaa gtaaaaatac atcatcgccg
     6361 cttgcaattt tattattatc ctcaaaacca tcaagttcta aaaacatttt tttagtatat
     6421 gcaaaattag caccattaca cataaaaggt tggttaatac caaaactacc aatagttgta
     6481 gcttgcaaac tcaacatttc aaactgttga aaatgatgaa accaattgtt ttttgtttta
     6541 tagattactg gtgcggctat catttcaaaa ctattttttt ggataaaacc atcgattgat
     6601 gataaccagt ttggatttac ttcacaatcg gcatccgtag taataatcca tggatttttg
     6661 gcaataggca tcgctcttga aatagcatct ttctttggcg aatttgacaa acgtaaattt
     6721 tccaataaag tagtgtcaat ttcatcattt ttcatgcgcc atttgataaa aacagcactc
     6781 gaattatctt tagaatcatc atcaatcata atgatttcaa tgaaattttt tggataattt
     6841 agattggaaa gtgacgctaa taattttgat aaattttttg cttcatttct aaatggaaca
     6901 acaatagtaa agttcgtttt cggagtatat tctttactta tcgattggtc ttttattttg
     6961 ttaaaaccaa aaaacagctg aagaataaaa acagcataca ccacaattag tataaaaaag
     7021 attatttcca tttggatttg aaatttaaga caaaaaaact tccaattaat acaggcaaaa
     7081 ctacatttaa aaaccacatt aatgtactaa caaagagaat aatccattcg ttaatatcat
     7141 gtttcccgaa aaaaaacaga gcaacacttc cttttacagc aaaatcaaac atttgaaaac
     7201 taggcaaaga agatgccaaa aaatagacaa ctgcaatggt tgaaattaaa gttatataag
     7261 gcaaatgaac gtctaaaaca acgaacagaa gataatactg atgcgaaaaa accacatatc
     7321 ttgaaagtcc taaaaacatg tttctttgat ggattttttt gggaatttca ttcgtttttt
     7381 gaagtacaga agcaatagaa tagtttttaa tcttgaagtt tttagtcaaa aaaacaatca
     7441 ccagcaccac aatacttatt ccaaaaagag taaatgcaat ttcataatac cctaaaacga
     7501 ttaacccaaa caatccaaat aggatggtta gtatcatttg aattccattg caaataaggt
     7561 ttaaaaaaat aattatcttt gattttgact tttcaaaaaa taatactttc cctgcgtatt
     7621 caccaatacc atttggggta attattccac cggttaatgc tgctaaaact tgttttgtag
     7681 cttcaaaaat agaaattgga gcaatataag aaactaaatt tttccacttt aaaatttcta
     7741 aatagcgatt taaaaaactt aaaaaaagta atgcactaat aatcccaaaa gaaaatttat
     7801 tttgaataat tccaacaaaa tcagtccaat taaaattatt tttttttgta atttgagtgt
     7861 atataaaata gaaagccaaa agaacaatac taatctttag aataagaatt acgagtttat
     7921 ttttactttt tgacatcatt tggcaaaata aactataaaa aataaagaaa cctatttaaa
     7981 aaaattagat tgttgccaaa atcaaggttt actccataat ttttaatact tttagaaact
     8041 attttttaat ataaagtacc cttaaaaatg aaaattaaat tacatttact tatcgtattt
     8101 cttagttttt cggctttttc acaaaatatt atttgggaaa aagtaacttc tagagaaata
     8161 actaaaaaca aatttgttga aagagacaac tttccaaatc aatttgattt gtatcaaatt
     8221 tcaacaagtg tactaaaaaa cactttactt caatctccaa accgactgca atcaaattcc
     8281 tctaaagtga ttatttcgat tccaaattta aacggagaat tggagcgttt tcagatgttt
     8341 gagttttcaa actttgcacc acaattgcaa gcacaatatc ctgaaattag atcttatgtt
     8401 ggccaaggaa ttgaggataa aactgcggtt ttaagattga gttccgaccc aagaggtttt
     8461 caaggaatga ttttaagagc aaataatgaa agtgaatttt ttgaacccta ctcaacggat
     8521 ggttcggtgt atgcatttta tgattctgaa agaaaaaatg gagacttgcc ttttgtttgt
     8581 tcaacacaag atttagcatt aaataacgaa ttaaaaagtg ctactactaa tcagagatct
     8641 aattcaggag aattattaac ctttagatta gcattgtctt gcaatggtga atatgctcag
     8701 tattttggtg ggtcagttgc tcaagcttta gcaggaatga atgctactat gacaagagtt
     8761 aatggtgttt ttgaaaatga ttttgccatt catatgaata taatctcaaa caacaatctt
     8821 gtagtatata ctaacccatc gactgatcct tattctacaa acttaggttc ttggaatggg
     8881 caacttcaaa acacattgac aaatataata ggagaagcta attatgatat aggccatatg
     8941 tttggagctt caggtggcgg tggagctgca ggctgtattg gttgtgtatg tgtagatggt
     9001 tcaaaaggaa gcggaataac ttctccagga actggtggac cagttggcga cacgtttgac
     9061 atagactatg ttgctcatga aatgggacat caatttggag ccaaccacac tttttcacac
     9121 aatgttgaag gctctggagt caatgtagaa ccaggttcag gttccacaat tatgggatat
     9181 gctggaatta cgtcaagaga tgttcaacca cattctgatg cctattttgt ttattcaagt
     9241 atcaagcaga ttcaagacaa tatggttgaa aaacactgtc cgcaaagagt taccttgtct
     9301 aatattgctc cgattgtaaa tggtggcttg gattatacaa tacccaaaag cacacctttt
     9361 gtattaacag gtgaagcttc agatactaat ggtgatatat tatcctattg ctgggaacaa
     9421 aatgatactg ctacaacaca aactggtgca aacagtggcg cttcaccaac aaaaacaaat
     9481 ggtcctaatt ttagatcata ctctccagta gtttcatcat ctaggtattt tccaagaata
     9541 caaagcattg tcgcaaacca agcaactact tctggaacag agataattgt ggaggctttg
     9601 agttctgttt caaggacatt aaactttgta cttacggtta gagataattt tgctggagca
     9661 ggtcaaacaa actctgatgc tatggttgta acagttaatg ctgctgctgg tccattcgta
     9721 gttacatcac caacaaccgc tgtgtcttgg acagttggta ctaatcaaaa cgtaacttgg
     9781 aatgttgctg gaacaactac taatgacatc aatgctgaat atgttgatat tttcctttca
     9841 acagatggtg gatttacgta cccaattcag ctagcaagta aagtgccaaa tgatggttca
     9901 gaattgatta ctgttccaaa taacataggc acaacgaata gaataatggt aaaaggttat
     9961 aaacacatct tttttgatat ttcaaatact aatttctcta ttattgctcc cacaagcagt
    10021 tttgcagtag catataatgg agttgcagaa caacaaaata aagaaatatg tagaggaagt
    10081 tcggtcacat atacaattcc atatactgct tatgctggtt ttagtggtac aacactattt
    10141 gctgtttctg gcgaacctga caattcaacc atcacttttt ctccaagtac tgcttctaat
    10201 agccaagaca taacgatgac cgttgaaact acaaacaact ctacacctgg agtttattca
    10261 atgctagtaa caggaatttc aggcacaacg actaattccg ctccatttta tttgaattta
    10321 tttgatgcta actttagttc cctaacctta acttctcctg cagaccaatc tattggtcag
    10381 caagtcgatt taaatttaac ttggaatatc gattcaaatg ctacgttata tgatgttcaa
    10441 gtttctacag aatctgactt ttcatctatt gtatattcag gaacggtttc aactaatagt
    10501 tttgctttaa ctaatttagc aagtgcaact cgatattatt ggagagtttt acccaaaaac
    10561 aactcttgtt ctggagtgta tagcggagct tatttgttta aaacaggtca aatcacttgt
    10621 accgattttt cttcaacaaa cgttccgatt actataccaa ctacagcaaa cgcaactgta
    10681 aattcaacct tgtctgttac taatacagat gttatttcag atgcaaatgt tactctgaat
    10741 atcgcacaca cgtgggttaa tgatttaaca attacgttaa taagcccagc aggaacagag
    10801 gttcagttag taggacaacc ttgtacatca gatgattttc aaaacattac agcaactttt
    10861 gatgattcag gaattccagt ggtttgtggt gctaatccag caatatctgg tactgtattt
    10921 ccggcccaag cactttcagc atttaatggt gaaactatga acggaacttg gacgttgagg
    10981 gttttagatt cttttaatca agatggtggt tcaataaatg gttggtcact aaatttatgt
    11041 agtaatactg ctgtaccact agcaacaact gaaaactcac ttcaaaactt cgctttatat
    11101 ccaaacccga ataatggaag ttttacagta tcatttaatt caaattctac aaataaaata
    11161 agtgttgatg tatttgatat tcgtggacga aatgtttttt caaatgaata tcaaaacaat
    11221 ggcatcttta atgagaacat tcaaatgagt acaattcaat caggaatata tttagtaaag
    11281 gttcaagatg gcgaacaaca gcttaccaaa aagattgtta tcaattaaac ttgattgaat
    11341 tttaaataaa aaaataccct caaaaagcta attgctattt gagggtgttt tttagaaatt
    11401 ttggagatag aagtttttga gtgtaaactt ctataaaatt ttccttttta tagttgtggt
    11461 cctgctttta ctaagttttg acctttttca ttatccgtat attgttcaaa attcttgatg
    11521 aatttatatg ctaaatcttc tgcttttaca ttccaatcat ttttatctcc atatgtatct
    11581 ctaggatcta aaattgctga attcacatta tttaattgtg ttggcacttc aaagttaaaa
    11641 acaggaattg ttttggttgg tgccttttct attgaaccgt caagaatagc atcaataatg
    11701 gcgcgagtat cttttatcga aattcttttc cctgttccat tccacccagt atttaccata
    11761 taggcagttg cattatttgc ttccattttc tttactaact cttccccata ttttgttggg
    11821 tgcaatgtta aaaatgcttt accgaaacat gcagaaaaag taggttgagg ctctgtaaca
    11881 ccgcgttctg ttcctgctaa ttttgctgtg aatcctgaaa gaaaataata tttagtttgt
    11941 tcaggtgtaa gttttgaaac tggtggcata actccgaaag catctgcggt taaaaagata
    12001 acttttgtag catgtcctgc ttttgaaact ggctttacaa tattttgaat atgatttata
    12061 gggtaagaaa ctctagtatt ctgagttaca cttccgtctt taaaatcgat aattccatca
    12121 gcatttaccg ttacgttttc taacaatgcg tcttttgtaa tcgcaccata tatatctggt
    12181 tcattttctt tgcttaaatc aatagtttta gcataacatc caccttcgaa attgaacact
    12241 ccttcattgt cccaaccgtg ctcatcatca cctattaatt ctctctttgg atctgtagat
    12301 agcgttgttt ttcctgttcc tgacaaacca aaaaatacgg caacatcgcc atcttttcct
    12361 ttattagccg aacaatgcat agaagctatt ccttgtaatg gcaaataata attcatcatg
    12421 gcaaacattc cttttttcat ttcgccacca taccacgtac cgccaataat ttgaattttc
    12481 tctgataagt tgaaagcaat atatacatca gaatttagat tgtaagcttt ataatcttca
    12541 aaatttgcct tagaagcatt aataactaca aaatctggtt caccaaaatt agctaattct
    12601 tcttctgtag gacgaatgaa catgtttttt acaaaatgtg cttgccatgc aacttccata
    12661 ataaagcgaa ctttcaaacg tgtattttca tttgcaccac aaaaagcatc tactacaaat
    12721 aatcttttgc ccgacaactg atttacagat gtttctttta aagcattcca agtgtcttga
    12781 gaaatgggtt tgttatcgtt aactgctttg tcagaattcc accaaatagt gttttctgtt
    12841 accgcatctt tcacgatgta tttatctttt ggtgagcgac cagtgaattc acctgtcatc
    12901 acatttactg ctcctagctc tgttaattgt cctttttcaa acccttttaa atttggacta
    12961 agttcttcgt tatacaaaaa ctcaaaagat ggattgtata taatttcttg tacatttttg
    13021 attccgtact tatcaagcga aatcgatttc gtaaattgag cagtattctc cataaaattg
    13081 tattgtgttg caattattaa attgactgca aagttaaaaa ttaattatta caacgttgta
    13141 gttcttcgtt aaattatatt tttttcttga taaagtgcat aaaaagaaat gtccaagcta
    13201 caattaaaag caatccgccg attggcgtta taggcccaag aaattttaat tcaaaaccag
    13261 agataaccct tgttgataac aaatagatag aacccgagaa aagcaaaacc cctgaaacga
    13321 taagatagaa aagtgtcttc ttaagtttct ctgtacaatg agaattgaaa ctaaggacca
    13381 ataaaaacaa tgcatgatac atttggtatt taacccctgt ctcaaaagag gaaagttgtt
    13441 ctactgacaa tctaactttt aaagcatggg caccaaacgc tcccaaaata attgccgtta
    13501 atcctaaaaa acttccaacg attgccattc ttctatccat gatgtgattt tttaaaaaaa
    13561 caaaattatc ggttatagca atacaatctg ttaaatttcg atactatttt tgaattttaa
    13621 cagcctcttc gttttatatt tgcatcttta aatcatcatc atgaaaaaaa tagcactatt
    13681 tattgtcttt gcatttatac tttcttcttt tacagcagat agtggttatg gcataaaatc
    13741 gggagaaaaa ctagtatatg ctgggtctta taatatgagt ggcttgttaa cacaaattgc
    13801 gcaagttact atgtcaaccg aaaatgttac tacttcaaaa agtaattatc tacacctttc
    13861 ttgcgagttg gctacatata caaaatggga ttctttcttt aaaataagag atatatatga
    13921 atcctatgta aacccaacga cgcttaaacc gagtttatac aaaagaagta ttgatgaagg
    13981 tggctatact ataaaagaaa aatatgtttt taaaggcact aatgtcaaga gttccgttac
    14041 caaaaaaaat cgtcctgaag tacataaatc atttaccatt ggcacaaata ctcaagatgt
    14101 tatttccctt ttttataaag taagaacttt ggatttctct aaattcaaaa cgggtcaaac
    14161 gcaatcgatg atgattgttt tt
//
LOCUS       DAOOBH010000052        13999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5636_length_13999_cov_5.134466, whole genome shotgun sequence.
ACCESSION   DAOOBH010000052 DAOOBH010000000
VERSION     DAOOBH010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13999
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5636_length_13999_cov_5.134466"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PK218_06730"
     CDS             <1..598
                     /locus_tag="PK218_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPW98234.1"
                     /translation="GSLSNVIAGLVLTYMRLFRIGDRVKIGDVVGDVIEKSLLVTRVK
                     TIKNEIISIPNSTVMSSHTINYSSETGSVGLILHSTVTIGYDVPWKKMHQTLIEAADR
                     TTLLLKEPKPFVLQTSLDDFYVSYQINAYTKEANKQAAIYSELHQNIQDCCNEVGIEI
                     LSPHYRAARDGNTTTIPENYLDKDYKAPSFNVNMKKEN"
     gene            598..798
                     /locus_tag="PK218_06735"
     CDS             598..798
                     /locus_tag="PK218_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98235.1"
                     /translation="MENKITEILYDPTIGKLVTLFIGIAVIWIVIKFIQKKLFTKIDN
                     VDNRYKAKKFGSFLGYIITIIL"
     gene            861..1496
                     /locus_tag="PK218_06740"
     CDS             861..1496
                     /locus_tag="PK218_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPW98236.1"
                     /translation="MAFALQEVIASFAGWLAIMFGDFYKTGDRVQLGGIKGDVMDIGV
                     LRTTIMETGQWVDGDLYNGRIVLIANSYIFKEPVFNYSGDFPFLWDEIKVPIQFGSNY
                     EKAKQIILNVGLEVAGDLTLESKEKWNSLQKKYLLENAQTEPMVSLIANDNWVEFTLR
                     YVVRYNKRRATKTDLFTKLLQQIEATNGEVKFASATFQIVETPDFNINLKK"
     gene            1498..2721
                     /locus_tag="PK218_06745"
     CDS             1498..2721
                     /locus_tag="PK218_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPW98237.1"
                     /translation="MEFFHLFSILIVLSAAFAYINFRFLKLPNAIGLMLVSLLFSFLI
                     LITGNYFPEVKTVIEHRLNEINFSELLLEGMLSFMLFAGAIHIKYADLKSEKLSIMLF
                     STISVVLSTVIIGYASFYLLNAFGLEVSLINAMLFGSLISPTDPIAVLSILKSAGVSK
                     SLETKIAGESLFNDGVAVVVFITILQLSKPDVDTSFIGVLSLFGQEAIGGILLGLVLG
                     WTGYKLVASIDHYQVEVLVTLAVVMGGYTFAHYTHVSGPLAMVVAGLITGNHGKAYGM
                     SKITAEYVDKFWELIDEILNAILFVLIGLELLIIETSKTIFIVSFILIGVILITRYIS
                     VYIPSIFIRLKEQMTKKTLVILTWGGLRGGISIALALSIPISLNRDIWVTITYVVVCF
                     SILVQGMTIAKVAKS"
     gene            2762..4894
                     /locus_tag="PK218_06750"
     CDS             2762..4894
                     /locus_tag="PK218_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPW98238.1"
                     /translation="MIKKIFVAAFLLTATLNHAQLKQITLEESVLQQGRKFGADKLTG
                     FQWIPNSADYVYFTDSWTKMVSDNALKSNRKELVKLSEINAVLGTNLRNFYGIQWIDS
                     NTILITEKSKYYSYSIPSKSGKLLYESPENAENTTLDNAKSKVAFTIDNNLYIINNEK
                     EIAITSNSDKNIVSGQTIARSEFGISGGIFWSPKSNFLAFYQKDETDVADYPLLDINE
                     TPGKLVSIKYPMIGQKSEKPKVGIYNLTTGKTVFITPKGNSDDYLTNLSWSSDEKYIL
                     IAEVNRGQNNMDLNLYNAISGTFVKTLINEKSNTWVEPEHAAFFPNANSNNFIWISEK
                     DGFNQLYYYSLEGKLIKQLTANKFPTREIIGTNASGTEIYFKATGSNATNMLVYKVDL
                     KGKQTLITKDEGVHNVSISSDGNWFFDEFSNHATPSKTLLYSKKGTAKTLLVSPNKYD
                     GYQIGTAEIKTIKSADGTTDLYTRLIKPSNFDAAKKYPVMVYVYGGPHAQMITNSYLD
                     GANLWMYWMAEQGYLVFTVDNRGSDNRGVAFESVIHRNVGTNEIEDQIKGVDYLKSLP
                     YVDGNRLAVHGWSYGGFMTTSLLTRKPDVFKVGVAGGPVIDWKWYEIMYGERYMDTPS
                     ENEAGFEEANVINHAKDLKAKLLLIHGTVDDVVVMQHNLALVKKFVELGYQNVDFFPY
                     PMHKHNVLGKDRVHLIDKVLHYIIENNK"
     gene            complement(4965..6230)
                     /locus_tag="PK218_06755"
     CDS             complement(4965..6230)
                     /locus_tag="PK218_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HPW98239.1"
                     /translation="MNFFTKTILLLFLCFSTAVKANFILLPMDDVSQKNHLKAYGITY
                     WCLDKNYKASWLLNYRGGSFLLPDAPEIRKECQIRGVSFEILSISEANQILDEISSPS
                     VNMETVVLEKAPKIAVYTPKGKQPWDDAVTMVLTYAEIPFTPIYDEEVLSDQLILYDW
                     LHLHHEDFTGQYGKFFGAYRNAPWYIEQKKDAEALAAKLGFAKVSQEKLAVAKKIRDF
                     VIGGGFMFAMCSATDSFDIALAAEGVDICEPMFDGDNSDANYQSKIDYSKSFAFKDFI
                     LERRPEKYEFSDIDMTEKRRIPMEKDYFTLMDFSAKWDVIPSMLCQNHTQLVKGFMGQ
                     TTAFDRSLIKSSVLVLGENQLNEEGRYIHGQKGKGFFTFFGGHDPEDYQHRVGDEPTV
                     LDLHPNSPGFRLILNNVLFPAARKKPQKT"
     gene            complement(6563..7225)
                     /locus_tag="PK218_06760"
     CDS             complement(6563..7225)
                     /locus_tag="PK218_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW98240.1"
                     /translation="MEPIKIILVDDEVLFRKGIFFLLQREPNFEIIFEASDGQELINY
                     LKNTTIHPDIIMMDLKMPTLNGVEATKIIRKDFPKINIIALTSYNTDTFIKNMIEIGA
                     SSYLIKNATPIEMVETITKVASQGFYYSEAVYKILNSEIQIDALNLELKFLKCDLSSR
                     EREVLTLICKQYSATEIAEKLCISARTVDGHRNNLLLKTEAKNIAGLVVFAIQNKMVL
                     LD"
     gene            complement(7228..8013)
                     /locus_tag="PK218_06765"
     CDS             complement(7228..8013)
                     /locus_tag="PK218_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPW98241.1"
                     /translation="MKTTVDIEFLNVIFYIFLGFLLMAIVIIVFFFKSRKKIIEKELE
                     KKNLELSYQKELLRAVIITQEEERKRIAQDLHDDISSKLNIVSLNTHLLKTPNLSENE
                     VKDITENIISLTTKALDSSRTIAHNLLPPVLDKFGFHAAIDELSLEYRTIKSVVVNYK
                     NETKNEIFSNEVQLHLFRILQELMNNSIKHGKASEISILVSKKAATNILVYTDNGIGF
                     ELEKLKNKSGLGIKNIESRVGFINGKMILKTSKNEGIYVMITF"
     gene            complement(8648..8962)
                     /locus_tag="PK218_06770"
     CDS             complement(8648..8962)
                     /locus_tag="PK218_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98242.1"
                     /translation="MNDNQIKLEEAQKWAENWRNKQPDVVKAFLIPKEDIIALYESIT
                     LNGAQDVRGYLGIRETGEYKMMFVAVDTKGNDRIDLGIYDLTQPCPNFCDLESPLYDL
                     KK"
     gene            complement(9240..10790)
                     /gene="dnaB"
                     /locus_tag="PK218_06775"
     CDS             complement(9240..10790)
                     /gene="dnaB"
                     /locus_tag="PK218_06775"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPW98243.1"
                     /translation="MENFKNINPIKVDKTTIINLEKGKLPPQALDLEEAVLGAMMIDK
                     KGVDEVIDILQPDAFYKEGHKHIFEAIFQLFTDSQPIDLLTVSAQLKKSGKLDLAGGD
                     FYLIQLTQKISSSAHIEFHSRIILQKFIQRSLIRISSEIIEESYDESTDVFDLLDKAE
                     SKLYEVTQGNIKRSSETAQSLVIQAKKRIEEIANKEGLSGIATGFDKLDKITSGWQPS
                     DLVIIAARPGMGKTAFVLSMARNMAIDFGHPVALFSLEMSSVQLITRLISSETGLSSE
                     KLRTGKLEKHEWEQLSTKVKNLEKAPLFIDDTPSLSIFDLRAKARRLSSQHGIKLIIV
                     DYLQLMTAGGSNGKGGGNREQEISTISRNLKALAKELEVPVIALSQLSRAVETRGSSK
                     RPLLSDLRESGAIEQDADIVSFIYRPEYYKIDEWDDDEHTPTAGQAEFIIAKHRNGSL
                     ENVRLKFIGNLGKFDNLEDFGSMYDDFPSRMNADENPFFTKNLPSANDAFGSNINTSE
                     DDDSDVPF"
     gene            complement(10932..11885)
                     /locus_tag="PK218_06780"
     CDS             complement(10932..11885)
                     /locus_tag="PK218_06780"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HPW98244.1"
                     /translation="MEYLDFELPIKELEDQLDKCLIIGQESDVDVSKTCKQIEKKLEE
                     TKKNIYKNLTAWQRVQLSRHPNRPYTMDHIKGFCGETFLELHGDRGFKDDKAMVGGLG
                     KIDGQSFMIVGQQKGYNTKTRQYRNFGMANPEGYRKALRLMKMAEKFNIPVLTLIDTP
                     GAYPGLEAEERGQGEAIARNIFEMCRLKVPIICVIVGEGASGGALGIGVGDRVLMMEN
                     TWYSVISPESCSSILWKSWEHKETAADALKLTSTDMKKQKLVDEVIPEPLGGAHYDRE
                     TTFKTVEKYIMKSYNELKVLSTTELIAQRMDKYSKMGEFKE"
     gene            complement(11990..12874)
                     /locus_tag="PK218_06785"
     CDS             complement(11990..12874)
                     /locus_tag="PK218_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW98245.1"
                     /translation="MLNDNAKSYLHLHLIVFIWGFTAILGALITLDAMPLVWFRMLLA
                     IVFILGFIYWKKMPLKVSKKALLQFIFAGIIIALHWFTFFKAIKISNVSVTLACLSSG
                     AFFASFIEPILFGKKIIWYEVLLGILVIIGLYIIFNVKGNYFYGIVLALTSAFLSALF
                     SVINSKFVKTHDATIISFYELSGGVVFFSILLLFTNKFSVEFFSLTFHDLLYLLILSS
                     ICTAYAFIASTAVMKHLSAYTVMLTINLEPIYGIILAVLIFDEKEKMSVGFYIGALLI
                     LVTVILNVIIKTKLKKRR"
     gene            complement(12861..13946)
                     /locus_tag="PK218_06790"
     CDS             complement(12861..13946)
                     /locus_tag="PK218_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPW98246.1"
                     /translation="MLKIIDKYILKRYLITFSVMLLMFIPIGIVIDVSEKINRIIANK
                     VPFTDVARYYLDFTIYFANLLFPIFLFLSIIWFTSKLANNTEVIAILSSGISFTRFLR
                     PYIVGATIVSLLALIMSIYLVPSASAGFNSFRRKYLNTYDNSSAKNETNVLRQVSKDE
                     YIFVTNFQEVSKTAFNFSMEKFKDDKLQYKLNATRLKYNPKDSTYTLFNYTKRKIGAY
                     SDIIESGEKKDTIFNFDLDDLTPEVYIAETLPLTELNSFIEKEKLRGSSNINVYLVVL
                     YKKYSLPVSAFILTIIAVAVSSMKRRGGMGMNLAIGIILAFSFVFLDKIFGVLAEASS
                     TPPLLAVWTPNIIFGILAIYLLRNAKR"
BASE COUNT         4800 a         2206 c         2109 g         4884 t
ORIGIN      
        1 cggttcatta tccaatgtta ttgcaggatt agtattgact tacatgcgat tatttagaat
       61 tggagacaga gttaaaattg gtgatgttgt tggcgatgtt atcgaaaaat cacttttagt
      121 cactagagta aaaactataa aaaacgaaat tatttctatc ccaaactcta ctgtaatgag
      181 tagtcatact ataaattata gtagcgaaac aggtagtgtt ggattaattt tacactcaac
      241 agttaccata ggttatgatg taccatggaa aaaaatgcac caaactctta ttgaagctgc
      301 agatagaact actttacttt taaaagaacc taaaccattt gttttacaaa caagtttgga
      361 tgatttttat gtttcctatc aaattaatgc ctacaccaaa gaagccaaca aacaagctgc
      421 catttattct gaacttcatc aaaatattca agattgttgt aacgaagtcg gtatcgaaat
      481 tctttcacct cattatagag cagctcgtga tggaaatacc actacaattc cagaaaatta
      541 tttggacaaa gactataaag caccaagttt taatgtaaac atgaagaaag aaaactaatg
      601 gaaaataaaa tcaccgaaat attatacgat ccaacaattg gaaaattagt aactctgttt
      661 ataggaatag ccgttatttg gattgttata aaatttattc aaaagaaact ttttacaaaa
      721 attgataatg ttgacaatcg ttataaagca aagaagtttg gaagcttttt aggctatata
      781 ataactatca ttttataact attgtttaca gcaataaact tggaggatta accgtagcgt
      841 ttggtgttgc cggtgctgga attgcatttg cgcttcaaga agtgattgct tcttttgcag
      901 gttggttagc tattatgttt ggtgattttt acaaaacagg tgatagagtg caacttggcg
      961 gaattaaagg tgatgtaatg gatattggtg ttttaagaac aacaataatg gaaaccggac
     1021 aatgggttga tggcgattta tacaacggaa gaatcgtttt gattgccaac agctacattt
     1081 tcaaagaacc tgttttcaac tattctggtg actttccttt tctttgggat gaaattaaag
     1141 ttccaattca atttggaagt aattacgaaa aagcaaagca aattatttta aatgttggct
     1201 tagaagttgc aggtgattta actttagaat caaaagaaaa atggaattca ttacaaaaga
     1261 aatatctact cgaaaatgct caaacggaac ccatggtatc cttaattgca aatgataatt
     1321 gggtagaatt cacgttacga tatgtagttc gatacaacaa aagaagagca acgaaaaccg
     1381 atttgtttac aaaactacta caacaaattg aagctacaaa tggtgaagtg aaatttgctt
     1441 ctgctacttt ccaaatcgta gaaacacctg attttaacat aaacctcaaa aaataaaatg
     1501 gaattttttc acctattttc aattctaatt gttctttctg ccgcatttgc ctatatcaat
     1561 tttaggtttc taaaacttcc taatgccatc ggtttaatgt tggtttcttt acttttttca
     1621 ttcctaattt taataactgg taattatttt ccagaagtta aaaccgttat cgaacatcgt
     1681 ttaaacgaga ttaattttag cgaattatta cttgaaggca tgttgagttt catgcttttt
     1741 gctggcgcta ttcacatcaa atatgccgat ttaaaatctg aaaaactcag catcatgctt
     1801 ttttcaacca ttagtgttgt tttgagtact gtaatcattg gttatgcttc attttacctt
     1861 ttaaatgctt ttggtttaga ggtaagttta attaatgcca tgctttttgg ttccttaatt
     1921 tcaccaaccg atccaattgc ggtattgagt attctaaaaa gtgctggtgt ttctaaatca
     1981 ttagaaacca aaattgctgg tgaatcttta tttaatgatg gtgttgctgt agttgttttc
     2041 attacaatac tacaattaag taaacctgat gttgacactt catttatcgg agtgctttca
     2101 ttatttggtc aagaagctat tggtggaatt ctgcttggtt tagttctcgg ttggacaggt
     2161 tataaattag tagcaagcat tgaccattat caggtggaag tattggtaac attagctgtc
     2221 gtaatgggtg gctatacctt tgcacattat acgcacgttt ctggcccatt agcaatggta
     2281 gttgctggtt tgataactgg aaatcatgga aaagcttatg gaatgtcaaa aattactgcc
     2341 gaatatgtag ataaattttg ggaattaata gatgaaattc taaatgctat attgtttgtt
     2401 ttgattggtt tagaattatt gattattgaa acaagtaaaa ccatctttat agtttccttc
     2461 attttaattg gagtcatttt aataacaaga tacatttccg tttatattcc gtcaatattc
     2521 attcgtttga aagagcaaat gacaaaaaag acacttgtaa ttttaacgtg gggtggtttg
     2581 cgtggtggta tttctatagc tttagcactt tcaataccta ttagtttaaa tcgtgacatt
     2641 tgggttacaa taacgtatgt agtggtttgt ttttcaattt tagttcaagg aatgactatt
     2701 gcaaaagtag ccaaaagtta aaatcaaatt ttttatattt acaaaaaata attacaacaa
     2761 catgattaaa aaaattttcg ttgcagcatt tctattgaca gcaactttaa accatgcaca
     2821 actaaaacaa atcacacttg aagaatccgt tttacaacaa ggcagaaaat ttggagccga
     2881 taaattaact ggattccaat ggattccgaa ttcagctgac tatgtttatt ttacagattc
     2941 atggaccaaa atggtttcag acaatgcttt aaaatctaat cgaaaagaat tggtaaaatt
     3001 atctgaaatt aacgcggtat taggaacaaa tttgagaaac ttttatggca ttcaatggat
     3061 agattcaaat actattttaa tcaccgaaaa atctaagtat tactcttatt ctattccttc
     3121 aaaatctgga aaattacttt atgaatctcc agaaaatgct gagaatacaa ctttagacaa
     3181 tgctaaatca aaagtggctt ttactatcga taataattta tatatcatta ataatgaaaa
     3241 agaaattgcc ataacatcca attcagataa aaatatcgtt tctggacaaa ccattgcaag
     3301 gagcgaattt ggaatttctg gaggaatttt ttggtcgcca aaaagcaatt ttctagcttt
     3361 ttatcaaaaa gacgaaaccg atgttgctga ttatccttta ttagatataa atgaaacacc
     3421 aggaaaatta gtttcaataa aatacccaat gattggacaa aaatcagaga aaccaaaagt
     3481 tggaatttac aatttgacta ctggaaaaac cgttttcatt acaccaaaag gaaattcaga
     3541 tgattattta accaatcttt cgtggtcatc cgatgaaaaa tacattttaa ttgccgaagt
     3601 aaatcgcggt caaaataata tggatttaaa tttgtacaat gctatttcag gaactttcgt
     3661 aaaaacttta atcaatgaaa aaagcaatac ctgggttgaa ccagaacatg ccgctttttt
     3721 tcctaatgca aactcaaaca attttatttg gatttcggaa aaagacggat ttaatcaatt
     3781 gtattattat tctttagaag gaaaattaat caagcaatta actgctaata aatttccaac
     3841 tagagaaatc ataggaacaa atgctagtgg aacagaaatt tacttcaaag caactggttc
     3901 aaatgcaaca aacatgttgg tttacaaagt tgatttaaaa ggaaaacaaa cgttgattac
     3961 caaggacgaa ggcgttcata atgtaagtat ttcttcagat ggtaattggt tttttgatga
     4021 gttttcaaat catgccacac catcaaaaac attgctttat agcaaaaaag gaactgcaaa
     4081 aacattatta gttagtccaa ataaatacga tggttatcaa ataggaaccg ctgaaatcaa
     4141 aacgataaaa agtgccgatg gaacaaccga tttatatacc agattaatca aaccaagtaa
     4201 ttttgatgcc gccaaaaaat atcctgtaat ggtttacgtt tatggcggac cacatgcaca
     4261 aatgattacc aattcctatt tagacggtgc caatttgtgg atgtattgga tggctgaaca
     4321 aggttatttg gtctttacag ttgataatcg tggttcggac aatcgtggtg ttgcttttga
     4381 aagtgttatt catagaaatg ttggtacaaa cgaaattgaa gatcaaataa aaggtgttga
     4441 ctatttaaaa tcattgcctt atgtggatgg aaatagactg gcagttcacg gttggagtta
     4501 tggcggattt atgactactt cacttttaac cagaaaacca gatgtgttta aagttggtgt
     4561 tgctggcgga ccagttattg attggaagtg gtatgaaatt atgtatggcg aacgttatat
     4621 ggatacgcca agtgaaaacg aagcaggatt tgaagaagcc aacgtaatca atcatgctaa
     4681 agatttaaaa gcaaaacttt tattaattca tggaacggtt gatgacgttg tggtaatgca
     4741 acacaatttg gctttagtga aaaaatttgt tgagttaggg tatcaaaacg tagatttctt
     4801 tccttatcca atgcacaaac ataacgtact cggaaaagac agagtacatt tgattgataa
     4861 agtattgcat tatattattg agaataataa ataagtgaaa tttcaaaagt taaattccaa
     4921 atcccaaagt ttagaattgg aatttggaat ttgaaatttt tatgttaggt tttctgcggt
     4981 ttctttctag cagctggaaa caatacatta ttcagaatca atcgaaaacc tggagaattt
     5041 ggatgtaagt ctaaaacggt tggctcatct cctactctat gctgataatc ttctggatca
     5101 tgacctccaa aaaaggtaaa aaatcctttt cctttttgtc cgtgaatgta tcttccttct
     5161 tcgtttaatt ggttttcacc caaaactaac acacttgatt taattaaact tctatcaaaa
     5221 gctgtagttt gtcccataaa acctttaacc aattgtgtgt gattttgaca caacatactt
     5281 ggaataacat cccactttgc tgagaaatcc attaacgtga aataatcttt ctccattgga
     5341 attctccttt tttccgtcat atcaatatct gagaattcat atttttctgg tcttctttct
     5401 aaaataaaat ctttaaaggc aaatgatttg gaataatcaa ttttcgattg atagttggcg
     5461 tcactattgt caccatcaaa catgggttcg caaatatcaa caccttcggc tgctagtgca
     5521 atatcaaaac tatctgttgc agaacacata gcaaacataa atccgccacc aattacaaaa
     5581 tctctaatct tcttggcaac ggctaatttt tcttgcgata cttttgcaaa acctaatttg
     5641 gcagccaaag cttctgcgtc tttcttttgt tcgatatacc aaggagcatt tctataagca
     5701 ccaaagaatt ttccatattg accggtaaaa tcttcatgat gcaaatgcaa ccaatcgtat
     5761 agaattaatt ggtcggacaa cacttcttcg tcatatattg gcgtaaaagg aatttcggca
     5821 taagttaaaa ccattgttac agcatcatcc caaggttgtt tcccttttgg tgtataaaca
     5881 gctattttag gtgctttttc tagcacaact gtttccatat ttacagatgg tgatgaaatt
     5941 tcatctaaaa tttggtttgc ttctgaaatg gatagtattt caaaactaac acctcgaatt
     6001 tggcattcct ttctaatttc tggagcgtca ggcaacaaaa atgaaccacc acgataattg
     6061 agcaaccaac tggctttgta atttttgtcc aaacaccaat aagttatacc ataagctttt
     6121 agatgatttt tttgagaaac atcatccatt ggcagcagta taaaattggc tttaaccgca
     6181 gttgaaaaac acaaaaaaag caataaaatg gtttttgtaa aaaagttcat tttcaaaatt
     6241 ttattcgaat atactatata aaatgaagtt ttacgtcaaa tttctgttaa aaataaaatc
     6301 taaaaacagg ttgatttgcg tatatacagt ccgattgtat ccattaattt tggaatatca
     6361 aaaagtcatt ataaaaacat tgtggttatg tctaaaagtt taaacaaatt gaattctgtt
     6421 gtaaaaatag ctttgttggt gctatttatg attattgtgg taaattttat aatggctttt
     6481 gatattatta ttgggtaaat tgagacttac ctatttttag aaaacgtggt ttatttgttt
     6541 tgaccacgtt tttttttaat ttttaatcta ataaaaccat tttattttga attgcaaaca
     6601 ctaccaatcc agcaatgttt ttcgcttctg tttttaataa taaattattt ctatgaccat
     6661 ctacagttct tgcgctgata caaagctttt cagcaatttc agtagcacta tactgtttgc
     6721 aaattaacgt taaaacttct ctttctctag aagacaaatc gcattttaag aattttaatt
     6781 ctaaattaag agcatcaatt tgaatttcag aattgagaat tttataaaca gcttcactat
     6841 aataaaatcc ttgactggca actttagtaa tcgtttcgac catttctata ggagtagcat
     6901 tttttattaa ataagaagat gcaccaattt ctatcatatt cttaataaat gtatctgtgt
     6961 tgtaacttgt taaagcaatg atgtttattt tgggaaaatc ttttctgatg atttttgtag
     7021 cttctacacc atttaaggtt ggcattttta aatccatcat gatgatgtcg ggatgtatgg
     7081 ttgtattttt caaataatta attaattctt gaccatcact tgcttcaaag ataatttcaa
     7141 aattaggctc tcgttgcaat agaaagaaaa ttccttttcg gaataatact tcatcatcaa
     7201 ctaaaataat ttttataggc tccatattta aaaagtaatc attacataaa tcccttcatt
     7261 ttttgaggtc tttaaaatca tttttccatt gataaaacca accctacttt cgatgttttt
     7321 tattcctaat ccacttttat tttttagctt ttctaattca aagccaatac cattatccgt
     7381 ataaaccaat atattagttg ctgctttttt acttaccaaa attgaaatct cactagcttt
     7441 gccatgtttt atagagttat tcatcaattc ttgtaagatt ctaaataaat gcaattgaac
     7501 ttcattagaa aatatttcat ttttggtttc gtttttatag tttacaacca ctgatttaat
     7561 cgttctatat tctagtgaaa gttcatctat tgcagcatga aatccaaatt tatccaaaac
     7621 tggaggcagc aagttgtggg caatcgtcct agaactatca agtgcttttg ttgtcaaact
     7681 tataatgttt tctgtaatat ccttcacttc attttcagat aaatttggtg tttttaaaag
     7741 atgtgtattc aatgaaacta tgttcaactt tgagctaata tcatcgtgta aatcttgagc
     7801 aatacgtttt cgttcttctt cttgtgtaat gataacggct cgtaatagtt ctttttgata
     7861 acttaattcc aaattctttt tttcaagttc tttctcaatt attttttttc gagatttaaa
     7921 aaagaaaacg ataatgacta ttgccattag aagaaatcct aggaaaatat aaaaaattac
     7981 atttaaaaac tcgatgtcaa ctgtcgtttt cattacttag attttaaagg ttttttggaa
     8041 taatttctta accattctat taagacaatt acttgaaaaa gaagatacaa tattgtatta
     8101 agtgaccaaa aaaacttaga gaaattaaaa tttatattat ttaaaatatt tccaaacaaa
     8161 aatactattg tacttccgaa aagataaaca attaatgcaa tggttatcat ataaaattct
     8221 tttttctttt ctagcatttc aaaaagatag aaagttgcat aaattattaa tggaaaagtt
     8281 gttataaata tctctagaag attaaactta aagaacaata ctggattaaa aacatattgc
     8341 aaaattaggg cactaaaaca aaccaacata tagttcaata ttattttttt ttggattctt
     8401 ttttcaatta attcataaaa aaagagactt agaaacaaaa actgcataac aaaataaatg
     8461 tgcgtcataa agtgattatt tagcttataa aaagataatg ttcgcatacc tatttctaca
     8521 actgtatgtg aaaaaatata taaagtaaaa aatttaaaag gtttcccaaa attaggataa
     8581 cttttaaagt aaaggataca atttagtatc aaaaggtaaa aagcaatgtc accagttatt
     8641 cttgccatta ttttttcaaa tcatataaag gactttccaa atcacaaaaa ttaggacaag
     8701 gttgagttaa atcatatatt cccaaatcga ttctgtcgtt acctttagta tctactgcta
     8761 caaacatcat tttgtattcg cctgtttccc gtattcctaa gtaaccacgt acatcttgag
     8821 ctccatttag agttatactt tcatacaatg caatgatgtc ttcttttgga attaagaatg
     8881 cttttaccac atcaggttgt ttatttctcc aattttctgc ccatttttgt gcttcttcta
     8941 atttaatttg attgtcattc ataatatctg atttaagttt gaattagttg gtaatttaag
     9001 cattattgac ttaaaatttt tcttgtttta aaaaaaataa ataaaagtat agagcaaaga
     9061 attaaagcaa ctataaaagt ggtcactatt gttttctgta taaaatagat gtcaaaattt
     9121 atacttttca tagttattgt tagctgttta gtgtttgtaa aattaaaaca aaaaaaaatg
     9181 ctaaaaccag gctattcaac ctgtttttag caacaaaata atattatttg aatttttatc
     9241 taaaacggga catcactatc atcatcttct gaagtattta tattactacc aaaagcatcg
     9301 tttgctgaag gaagattttt ggtaaaaaaa ggattttcat ctgcattcat tcttgaagga
     9361 aaatcatcat acatactacc aaaatcctca agattatcaa attttcctag atttccaata
     9421 aatttcaaac gcacgttttc aagcgaacca tttctgtgtt ttgcaataat aaattcggct
     9481 tgacctgcag ttggcgtatg ctcgtcgtca tcccattcgt caattttata atattcagga
     9541 cgataaataa acgaaacaat atcagcatct tgctcaatag ctccagattc acgtaggtcg
     9601 gacaacaaag gtcttttact tgaaccacgt gtttcaacag cacgtgataa ttgagacaaa
     9661 gcaatcactg gcacttccaa ttcttttgct aatgctttta ggtttctaga aatagtagag
     9721 atttcctgct ctcgatttcc gccacctttt ccattacttc cacctgcagt catcaattga
     9781 aggtaatcca caatgattaa ttttatacca tgttgtgaag acaaacgtct tgctttagca
     9841 cgtaaatcaa atattgaaag tgatggtgta tcgtcaataa acagtggtgc tttttctaaa
     9901 ttttttactt tagttgaaag ctgttcccat tcgtgttttt ctaatttacc tgttcttagt
     9961 ttctctgatg acaaacctgt ttcagaggaa ataagacgtg taattagttg aacagaagac
    10021 atttctaaag aaaacaatgc tacaggatga ccaaaatcga tagccatatt acgtgccata
    10081 gaaagtacaa atgctgtttt ccccatacct ggacgagctg caataatcac taaatcagaa
    10141 ggttgccatc cagaagtgat tttgtctaat ttatcaaatc ctgttgcaat accgcttaaa
    10201 ccttctttat tagctatttc ttcaattcgt ttttttgctt gtataaccaa actttgtgcc
    10261 gtttcagaac ttcttttaat gtttccttgt gttacttcat atagttttga ttctgctttg
    10321 tctaataagt caaaaacatc ggttgattca tcatacgact cttcaataat ttctgatgat
    10381 attctgatta aacttctttg aataaatttt tgcagaataa ttcttgaatg aaactcaata
    10441 tgtgccgaag acgaaatctt ttgtgtaagt tgaattaagt aaaaatcacc acctgccaaa
    10501 tccaattttc cactcttttt gagctgagca gaaactgtta ataagtcaat tggctgagaa
    10561 tctgtgaata actgaaaaat ggcttcaaaa atatgcttgt gtccttcttt gtaaaaagca
    10621 tcaggttgca aaatatcaat aacttcatca actccttttt tatcaatcat catggctcca
    10681 agaacagctt cctcaaggtc taaggcttgt ggtggtaatt ttcctttttc taggtttatt
    10741 atagtagttt tatctacttt tattggattg atatttttga aattttccat tgagcgaaag
    10801 tacttttttt taaacagaaa ttaaagttga gttatacaat ttaactgttg aaaagttatt
    10861 tttttttgtt gataaccata aaaaaatccg aaacgattgg gtttcggatt aagaactatt
    10921 ttgtatgatt tttactcttt aaactcaccc attttactat acttgtccat cctttgggca
    10981 ataagttcgg ttgttgataa aacttttagt tcgttataag atttcataat atatttttct
    11041 actgttttaa aagtcgtttc tctgtcataa tgcgctccac caagtggttc tggaataact
    11101 tcatcaacta atttttgttt tttcatatca gtagaagtta attttagagc atcggcggca
    11161 gtttctttgt gttcccagct tttccataaa attgaagagc aagattctgg agaaattacg
    11221 gaataccaag tattttccat cattaaaacc ctgtcgccaa cgccaattcc taatgcaccg
    11281 ccagaagcac cttcgccaac aataacacaa ataattggta ctttaaggcg acacatttca
    11341 aaaatatttc tagcaattgc ttcaccttgt ccgcgttctt cggcttctaa tccaggataa
    11401 gctccaggtg tatcaattaa agttagtaca ggaatgttaa atttttcggc catcttcatt
    11461 aagcgcaatg cttttctata accttccgga tttgccattc caaaatttct atattgacgt
    11521 gttttggtat tgtatccttt ttgttgtccg acaatcataa aggattgtcc atcaattttt
    11581 ccgagtcctc caaccattgc tttatcgtct ttgaaacctc tatctccatg aagctctaaa
    11641 aaagtttcgc cacaaaaacc tttgatgtga tccatagtgt aaggtctatt tggatgtcga
    11701 gaaagttgaa ctctttgcca ggcggttaaa tttttgtaga tatttttctt tgtttcttct
    11761 aacttttttt caatttgttt gcaggtcttt gaaacatcta catccgattc ttgtccaatt
    11821 attaaacatt tatctaactg gtcttcaagt tcttttatag ggagttcaaa atctaaatat
    11881 tccataaaat tgattgtttt atttttatga aagcaaatat aaaattttaa attctgaaac
    11941 agttaaaact atttaatatt ttatcgcaaa attggcaaaa acatcaagtt tatcttcttt
    12001 ttttaagttt agtttttata atgacattca atataacagt gactaaaatt agtaaggcac
    12061 caatgtaaaa gccaacgctc attttttctt tttcatcaaa aataagtact gctaaaatta
    12121 ttccataaat tggctctaga ttgattgtaa gcataacagt ataagcactt aaatgtttca
    12181 taacggctgt agatgcaata aaagcataag ctgtacaaat ggaacttaaa attaataaat
    12241 acagtaaatc atgaaatgtt aaactaaaaa attcaaccga aaatttatta gtaaacaaca
    12301 ataatatcga aaagaataca actccgcctg atagttcata aaatgaaata atcgtggcat
    12361 cgtgtgtttt tacaaattta ctattaataa cagaaaacaa cgctgataga aatgctgatg
    12421 ttaaggctaa tacaattcca taaaaataat tcccttttac attaaaaata atatacaatc
    12481 ctataataac taaaattcca agcaatactt cataccaaat gatttttttt ccaaataaaa
    12541 taggttcaat aaaagaagca aaaaaagcac ccgaagacaa acaagccaat gttactgaaa
    12601 cattggaaat tttgatggct ttaaaaaatg taaaccaatg taaagcaatt attatcccag
    12661 cgaaaatgaa ttgaagtaat gcttttttag aaacttttaa aggcattttt ttccaataaa
    12721 tgaatcctaa aataaatact atcgctaaaa gcattctgaa ccaaactagt ggcattgcat
    12781 caagtgtaat taacgcgcca agtatagctg taaaccccca aatgaatact atcaaatgaa
    12841 gatgtaaata gctttttgcg ttatcgttta gcatttcgaa gtaaataaat ggctagaatt
    12901 ccgaaaataa tatttggagt ccaaacggct aaaagtggtg gcgtactaga agcttcggca
    12961 agcactccga atattttatc taaaaacaca aagctaaatg ccaaaataat tccaatggct
    13021 aaattcattc ccattcctcc acgacgtttc atcgaagaaa ctgctactgc aattatagtt
    13081 aaaatgaaag cagaaacggg taaactgtat tttttatata gtacaaccaa atagacatta
    13141 atatttgaag atcctcgaag cttttctttt tctataaaac tattcagttc cgttaacggt
    13201 aaagtttctg caatgtaaac ttctggcgtt aaatcatcta aatcaaaatt aaaaattgta
    13261 tctttttttt caccactttc gataatatcg ctataagcac cgatttttct tttggtataa
    13321 ttaaataaag tataagtgct atcctttgga ttatatttta atcttgtagc attcagcttg
    13381 tattgtagtt tatcgtcttt gaatttttcc atcgaaaaat tgaaagctgt tttagaaact
    13441 tcttgaaagt ttgtgacaaa gatgtattca tctttactaa cctgtcgaag tacgttagtt
    13501 tcgtttttgg cactcgaatt gtcataggta tttaaatact tacgcctaaa tgaattgaat
    13561 cctgcactag cacttggcac aagataaata ctcattatta atgccaataa agaaactata
    13621 gtagcaccca caatatatgg acgtaaaaag cgagtaaatg aaattcctga acttaaaatg
    13681 gctattactt cggtattgtt tgcgagtttt gaggtaaacc aaattatgga taaaaataaa
    13741 aatatcggaa acaataagtt ggcaaaataa atagtgaaat ctaaataata tcgagcaaca
    13801 tcagtaaaag gtactttatt tgcaataatt ctattgattt tttctgaaac gtcaataaca
    13861 attccgatag gaataaacat caacaacatt actgaaaatg ttattaaata gcgtttgaga
    13921 atgtatttat caatgatttt taacatctat tataatcgtt gattcatttg ttttaccatc
    13981 atttctttcc aagttctaa
//
LOCUS       DAOOBH010000053        13874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5689_length_13874_cov_7.271293, whole genome shotgun sequence.
ACCESSION   DAOOBH010000053 DAOOBH010000000
VERSION     DAOOBH010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13874
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5689_length_13874_cov_7.271293"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1105
                     /locus_tag="PK218_06795"
     CDS             149..1105
                     /locus_tag="PK218_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPW98247.1"
                     /translation="MFVINNIKVLHLDSNHPLLWNQLEQAGFLNEHDYTSTKEEVASK
                     IHNYDGIIIRSRFKIDREFIDKATNLKFIARVGAGLESIDSEYAIAKKIHLIAAPEGN
                     KNAVGEHALGMLLSIMNKLNKADKMIRIGQWIREGNRGYELDGKTVGIIGYGNMGKAF
                     AKKLRGFEVEVLCYDIQENVGDENAKQVSLRELQKRVDVLSLHVPWTRETDKMMKNEF
                     INSFSNPFWFINTSRGKNVVTDDLVKALASKKILGAGLDVLEYEKLSFENLFVDAERP
                     KALEYLLRAENVLLTPHVAGWTYESHEKLAETIVSKIIKLYE"
     gene            1098..1496
                     /locus_tag="PK218_06800"
     CDS             1098..1496
                     /locus_tag="PK218_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW98248.1"
                     /translation="MSKKDLKRVTGLGGFFFKSENPDQIKDWYKNHLGIPVDDYGCTF
                     WWKDENGNKCSTQWSPFEAKTDYFKPSQKQFMMNFRVDNLVALLAALKEEGVTIVGEM
                     EEYEYGKFAWVLDPEGNKVELWEPIDAPFL"
     gene            1596..1934
                     /locus_tag="PK218_06805"
     CDS             1596..1934
                     /locus_tag="PK218_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HPW98249.1"
                     /translation="MEAQKVDMFMMMNAKFFESHHVHAVREKLLSLDESKWGIVQSLQ
                     FKDPTTSLIVSLLGGGLGIDRFMIGDTGLGVGKLLTCGGLGIWTIIDWFMIMGATREK
                     NMENFQNSLY"
     gene            2103..2342
                     /locus_tag="PK218_06810"
     CDS             2103..2342
                     /locus_tag="PK218_06810"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98250.1"
                     /translation="MLLSKGALLESLQQNPFGILVAFSMLIFPFWIVYDVLKKTDTFY
                     HFYLKTESVIRKPKVALVLILLVILNWIWNLYKHL"
     gene            2339..2776
                     /locus_tag="PK218_06815"
     CDS             2339..2776
                     /locus_tag="PK218_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPW98251.1"
                     /translation="MITEKPFAYKPSEHEREKASNSYLMSLVALIAGLPLPIINLLAT
                     FFFFLANRKGTYFVRWHCTQALLSQLALLGINSASFWWTVSIIFGDEKVSNQYFAYIF
                     TVIIFNILEIISTIYAAIQTRKGKHLEFLFFGRITNLICKPRK"
     gene            2773..3570
                     /locus_tag="PK218_06820"
     CDS             2773..3570
                     /locus_tag="PK218_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPW98252.1"
                     /translation="MKNTVIQGLVILLSFFLIWFGFSQLDYMKFFKIKERTLNVENKL
                     GDLIWDQISRTEDVVLNDSITKTLDSLLLPICNANGIERDSLKVHIVKKDEINAFALP
                     NKHLVVYTGLIIDCKKQEALQGVLGHEIAHIENNHVMKKLSKELGFSVLMTAASGGKG
                     GQMIREVFKTLSSSAYDRTLEKEADMTSVDYMIKANIDPKPMADFMYKMALDNQMPKG
                     LAWISTHPDSEERAKYILEYIKGKKLTIKPTLIEKDWNHFQEQVKDL"
     gene            complement(3611..5404)
                     /locus_tag="PK218_06825"
     CDS             complement(3611..5404)
                     /locus_tag="PK218_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW98253.1"
                     /translation="MGLVTAKEVAKAINMDKYGVFGTFSGWLLMKALRISTLNDVYDR
                     NKHLSEVEFLNAILDEFQIKFEIPEEDLKRLPKDGAYITISNHPLGGIDGILLLKLML
                     EREPNFKIIANFLLHRIEPMKQYIMPVNPFEDRKDAKSSVIGIKETLRHLSDGKPLGM
                     FPAGEVSTYKDGKFVVDKAWEEGAIKVIRKAQVPVVPIYFHAKNSRFFYFLSKLNPKL
                     RTAKLPSELLTQKDRVIKVRVGKPISVAEQNEHKTIEDYSEFLRKKTYMLANSFNEES
                     KLIHVPNLKTPKSPKTIVTPANQDNMVAEVEKLRQTDCRFFQSKNYEVFFTEAHKIPN
                     ILHEIGRLREITFREVGEGTNESIDLDKYDQYYHHMFLWDEDTKQIAGAYRMGLGSEI
                     FPKLGMEGFYLNDLFRFETELHDMMSKSIEMGRAFIIKEYQQKPMPLFLLWRGIIHTT
                     LRYPEHKFLLGGVSISNQFTDFSKSLMIEFMKSNYYDPYIAQYVHPKKEFKVKLKDAD
                     KDFIFDETEADLNKFDKIIDELEPGNLRLPVLIKKYIKQNARLVAFNVDPLFNNAIDG
                     LMYIRIADIPESTMKPVMEEFQAELERKEKN"
     gene            5654..6415
                     /locus_tag="PK218_06830"
     CDS             5654..6415
                     /locus_tag="PK218_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPW98254.1"
                     /translation="MKIISYNVNGIRAAINKGFVEWLEQANPDVICLQEIKATEDQVP
                     VNELTQAGYPYQYYYSAQKKGYSGVAILSKIKPNHVEYGTGVDHMDFEGRNLRVDFDE
                     LSVMSLYLPSGTNIDRLDHKFKYMDDFQHYILSLRNTIPNLVICGDYNICHEAIDIHD
                     PVRNKTVSGFLPEERAWLDGFMKTGMIDSFRHFNKEPNHYSWWSYRANARNNNKGWRI
                     DYCLVAQPLENRLKRAVILPEAKHSDHCPVLVELT"
     gene            6449..7414
                     /locus_tag="PK218_06835"
     CDS             6449..7414
                     /locus_tag="PK218_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW98255.1"
                     /translation="MIKKTLLTGLLLLTLSSCVSKKIYNDLENKYTDLKKENNNMADE
                     LESLRKTKTEFETENASLKSELAKLKAERDKLQTDCTATANNLKALKESYNALEKNSS
                     EALTSNMNKNRELLAQLDTKEKALAAEQNRLNKLRDELSASSKRLSELESMIANKDAA
                     MKKLKETLSKALNAFEGKGLTVEQKNGKVYVSMENKLLFQTGSWAVGVEGKKAVVEVG
                     KVLAQNPDITVLIEGHTDNDKILGTLGGGIENNWDLSTKRATAIVNILAENAGIQKKN
                     LTAAGRGEFAPLMSNDTAEGKAKNRRIEIILTPKLDEISKMLNDF"
     gene            complement(7605..9038)
                     /locus_tag="PK218_06840"
     CDS             complement(7605..9038)
                     /locus_tag="PK218_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW98256.1"
                     /translation="MKYIKKNKIVALALVCLFFTSCKMLAPVSAIEKQPVPESYAVSK
                     DTLNSAKIKWKDFFKDKNLDNLIDIALKNNIELAVTLQEIEIARNDVRVKKGLLLPSV
                     NAVAAAGVDKVGRYTSTGAGDASTEITPGNEVPEVLQDFMLGLKATWELDIWKKLRNS
                     KKAAYSRYLGTVEGKNFVVTNLVAEIANSYYELVALDNQLDIVKETIALQKNALEIVK
                     VQKEAARVTELAVKKFEAEVLNSQSREYEILQQIKENENKINFLLARFPQPIVRDKTS
                     FMAEIPQQIQAGIPSQLLINRPDIKQAELELFAAKCDVKAAQAEFYPSFTISGGVGFN
                     AFKTSYLFTTPESLIYNLVGDLTAPLINRNAIKAEFNKANAMQLQAMYEYQKSILNGY
                     VEVSNELSNISNLEQFYTLKNKEVQALTTAIAISNDLFKSSRVDYFEVLMTQRDALES
                     KLELIETKKNQLIAVTNIYKALGGGWN"
     gene            complement(9025..12198)
                     /locus_tag="PK218_06845"
     CDS             complement(9025..12198)
                     /locus_tag="PK218_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW98257.1"
                     /translation="MFNKFIQRPVLSIVISLIILFLGFLALVQLPITQFPSISPPKVN
                     IVAEYPGANGELMIKSVVIPLERAINGIPGMKYISSDAGNDGEASIQVVFELGTDPNI
                     ASVNVQNRVAAVVNKLPPIVVREGVKITREESNMLMYINLYSKDPNLDVNFLYNYADI
                     NLLSELKRVDGVGVADVLGDREYSMRIWLKPDRMLAYKVSSEEVMKSLESQSFEASPG
                     KLGESSGKKSQAFEYVIKYPGRYTTQEQYENIVIRSNPNGELLRLKDVAQIEFGNEFY
                     NLYSKLNGKPSAAIVIKQSYGSNASKVIENVKKKLEEIKAESFPKGMDYEIGYDVSKF
                     LDASIEKVIHTLIEATLLVSLVVFIFLGDWRSTFITIIAVPISLVGTFFFMQLFGITI
                     NLITLFALVLAIGIVVDNAIVVVEAVHAKMEENNVDALEATKMAMKEIAGAIIAITLV
                     MAAVFIPVSFMSGPVGIFYRQFSVTMATAIILSGVVALTFTPALCAMMLKNNHGQEKK
                     KTVLNTLLARFNNWFNGLQTKYQKLVSAIVNRRVITILVLVAFCLGTWGLSTGVPSGF
                     IPNEDQGLFYAIIQTPPGSTLERTDKVADELQEIASKMDEVKSVSTMAGYEILSEGTG
                     SNSASCLINLKDWNDRKHNTQQIMDELEEKSKNIKGASIEFFPPPAVPGYGAAGGFEL
                     RVVDKGGSGDYKKMESVTKNFVKELSKRPELTSVFSFYSASFPQYMLKVDNDKAQQKG
                     VSIDDAMDNLSILIGSTYETSFIKYDRQYKVMVQALPQYRAVPEDILRLYTKNEEGEM
                     VPYGAFMTVEKVYGLSEITRHNLYNAAEISGGPAAGYSSGTALKVIADVAAKSLPKGF
                     GIEWAGISIDESRQGNQAIYIFLISLVFVYLVLSAQYESFMLPLPIILSLPVGIFGAF
                     FLLNVLGLENNIYAQISMVMLIGILGKNAVLIVEFAVQRHAAGLSAKKAAIQGATARL
                     RPILMTSFAFIAGLIPLVFATGPGAVGNRTIGAAAAGGMIFGTVFGVILIPGLYFLFA
                     KISEKHLLVEDEEENPITEEMNSNEIH"
     gene            complement(12229..13311)
                     /locus_tag="PK218_06850"
     CDS             complement(12229..13311)
                     /locus_tag="PK218_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW98258.1"
                     /translation="MNKLVLLISFCALSLLSSCTTKEKENKEVAEYIVTNPIVKDTTY
                     AKEYIAQIQSIQNVELRAQEKGYLENLYVDEGSNVKAGQLLFTIMPKLYEADYLKAKA
                     EAKIAALELQNTKTLADKNIVSKSELAMAQAKLEQANAELEMAETHLSFTKIKAPFDG
                     TIDRIRFKKGSLIDEGTVLTTISNNKDIFAYFNMSEPEYLEYKSRPKNDITNSVSLLL
                     SNNDLHKYKGVIETVESEFDNNTGNIAFRAKFPNPDYLLKHGETGKVLMTTPVKNALI
                     IPQKATFEVQDKIYVYVVDVNNKVHSQNIIVKQRLSNIYIVASGISEKERILVEGIQM
                     VKEDEKIIPKEVSPDKILKDLQLIQQ"
BASE COUNT         4592 a         2309 c         2371 g         4602 t
ORIGIN      
        1 atgtaattgt aacacaagct gacgaaacac gaaccgttag agttgtaaaa agatagtgta
       61 aaaaattatt aaaccccaat caagaaattt gattggggtt tttttatgga ttcaataaat
      121 agttatcttt acattctaat ccaaagatat gtttgtaatc aataacatta aagttctcca
      181 tttagactca aaccatccat tactttggaa tcaattggaa caagctggtt ttttgaatga
      241 acatgattat acctcaacta aagaagaagt tgcttctaaa attcataact atgatggcat
      301 tataattaga agtaggttta aaatagacag agagtttatt gataaagcca caaatttaaa
      361 attcattgca agagtaggtg ctggtttaga aagtatcgat agtgagtatg ctatagctaa
      421 aaaaattcac ttaatcgcag cacctgaagg aaataaaaac gctgttggag aacatgcgct
      481 aggcatgtta ttgtctataa tgaataaact taacaaagcc gataaaatga ttcgtattgg
      541 tcagtggatt cgtgaaggca atcgaggtta tgagttagac gggaaaacag ttggaattat
      601 aggatacgga aatatgggaa aagcttttgc aaaaaaactc cgtggttttg aagtggaggt
      661 actctgttat gatattcaag aaaacgttgg cgatgaaaac gcaaaacagg tttcattgag
      721 agaattacaa aaaagagttg atgttctaag tttgcatgtt ccttggacac gcgaaacgga
      781 taaaatgatg aaaaatgaat ttattaattc tttttccaat ccattttggt tcatcaatac
      841 ttcgagaggt aaaaatgtag taacagatga tttggttaaa gcattagctt caaaaaaaat
      901 tcttggtgct ggactagatg ttttggaata tgaaaagcta tcctttgaaa atttatttgt
      961 tgatgccgaa agaccaaaag cacttgaata ccttcttcga gctgaaaatg tattattaac
     1021 accacatgtt gcaggttgga cctacgaaag tcatgaaaaa cttgcagaaa cgattgtgag
     1081 taaaattatc aaattatatg agtaaaaaag accttaaaag agtaactggt ttgggcgggt
     1141 ttttctttaa atctgaaaac ccagaccaaa ttaaagattg gtacaaaaac catttgggaa
     1201 tccctgtaga tgattatggc tgtacattct ggtggaaaga tgaaaatgga aacaaatgta
     1261 gcacgcaatg gtcgcctttt gaagcgaaaa cggattattt taaaccaagt caaaagcaat
     1321 ttatgatgaa ctttcgggtt gataatttag ttgctttgtt agctgcttta aaagaagagg
     1381 gtgtaacaat cgttggtgaa atggaagaat atgaatacgg aaaatttgct tgggttttag
     1441 acccagaagg taataaagtg gaactttggg aaccaattga cgcgcctttt ttgtaacaaa
     1501 acattttttt ttcaacttat ataaaaaatg agtatcattt tataagaata ttatatattc
     1561 gtgaaaatat ttaaccaacc aaaaatcaat aaaaaatgga agcacaaaaa gtagatatgt
     1621 tcatgatgat gaacgcaaag ttttttgaaa gtcatcatgt tcacgctgtt agagaaaaat
     1681 tattaagttt agacgaatca aaatggggaa tcgttcagtc acttcagttt aaagacccaa
     1741 caacatcgtt aatcgtttcg ttacttggtg gaggattggg tattgaccgt tttatgatag
     1801 gcgatacagg tttaggtgtt ggaaaacttc ttacttgtgg tggtttgggt atttggacta
     1861 ttatcgactg gttcatgatt atgggtgcca ctcgtgaaaa aaacatggaa aatttccaaa
     1921 attcacttta ttaataaatg acacgaaaca ggttatacgt tataattctt atagcctgtt
     1981 tttttggtac actttatttt ttatatcatt caactgtagc aaattcaaca gatttaagtg
     2041 tatgcttaat taaaaacgta acaggctatc cttgtccatc atgtggtaca actcgagcaa
     2101 tgatgttatt aagcaaggga gcactgttgg aatcattaca gcaaaatcca tttggaatac
     2161 tcgttgcttt ttccatgctg attttccctt tttggattgt ttatgatgta cttaaaaaaa
     2221 cagatacttt ttatcatttt tatttaaaaa ctgaatcagt tattagaaaa cccaaagtag
     2281 ctttggtatt aattttgcta gttattttaa actggatttg gaatttatac aaacatttat
     2341 gattacagaa aaaccttttg catacaagcc ttcagaacac gaacgcgaaa aagcatccaa
     2401 cagttatttg atgtctttag ttgcgttaat tgctggtttg cctttgccaa ttattaacct
     2461 tttggcaacc tttttctttt tcttagccaa ccgaaaagga acttattttg tgcgatggca
     2521 ttgcacacaa gccttgttgt ctcaattggc acttttagga attaatagtg ctagtttttg
     2581 gtggacggtt tctataatct ttggtgatga aaaagtatct aatcaatact ttgcctatat
     2641 ttttacggtg attattttta atattttaga aattatttct acgatatatg ctgccattca
     2701 aactagaaaa ggaaaacatt tagagttttt gttttttggc agaattacta acttaatttg
     2761 caaacctaga aaatgaaaaa tacagtaata caaggattag tcattctgtt gagtttcttc
     2821 ctaatttggt ttggattttc gcaattggac tatatgaaat tctttaaaat taaagaacga
     2881 acactaaatg tagaaaacaa attaggcgac ttaatttggg atcaaatctc aagaacagaa
     2941 gatgttgttt taaatgattc tataacaaaa acgttagatt cattgctttt acctatttgt
     3001 aatgccaatg gaattgaacg tgattcattg aaagttcata tcgtaaaaaa agacgaaata
     3061 aatgcttttg cattacccaa taaacattta gtagtttata cagggttaat tatcgattgc
     3121 aaaaaacagg aagcactaca aggtgtttta ggtcatgaaa ttgcgcatat cgaaaacaat
     3181 catgtgatga aaaaattgtc taaagagctt ggcttttcag tattaatgac tgccgcttct
     3241 ggtggaaaag gaggacaaat gattagagaa gtattcaaaa cactttcttc ttctgcttat
     3301 gacagaactt tagaaaaaga agcggatatg acgagtgtag attatatgat aaaagccaat
     3361 attgatccta aaccaatggc agattttatg tataaaatgg cattagacaa tcagatgcca
     3421 aaaggattgg cttggatttc tacgcatccc gattctgagg aacgtgctaa atatatttta
     3481 gagtatatca aaggaaagaa gctaactatc aagcccactt tgattgagaa agattggaac
     3541 cattttcaag aacaagtaaa agatttataa attgaaatcc tgagcaatcg ggattttttt
     3601 atacaaacta ttaatttttt tcttttcgtt ctagttctgc ttgaaattct tccataacgg
     3661 gtttcatggt gctttctgga atatcagcaa ttctaatata catcaatcca tcgatagcat
     3721 tgttgaataa tggatctaca ttaaaggcta ctaaacgagc attctgtttg atgtattttt
     3781 taatcaacac aggcaaacgt aaatttcccg gttctaattc gtctatgatt ttatcaaact
     3841 tattcaaatc agcttcggtt tcatcaaaaa taaagtcctt atctgcatct ttaagcttta
     3901 ctttgaattc ttttttaggg tgtacatatt gtgcaatata aggatcataa taattagatt
     3961 tcataaactc aatcatcaaa gactttgaga aatcggtaaa ctgattgcta atactaacac
     4021 cgcctaataa aaatttatgt tcaggataac gcaaggtagt gtgaatgatg cctcgccata
     4081 aaagaaacaa aggcattggt ttttgttggt attctttaat aataaaagca cgtcccattt
     4141 cgatagattt tgacatcata tcgtgtaatt cggtttcaaa acgaaataaa tcattcaaat
     4201 aaaagccttc cattccaagt ttaggaaata tttccgaacc caatcccatt cggtaggctc
     4261 cagcaatttg tttggtgtct tcatcccaaa gaaacatatg atggtaatat tgatcgtatt
     4321 tatctaagtc gatagattcg tttgttcctt cgccaacttc acggaaagta atttcacgta
     4381 atcgcccaat ttcgtgcagt atgtttggga ttttgtgtgc ctcagtaaaa aaaacttcat
     4441 agtttttact ctgaaaaaaa cgacaatcgg tttgtcttag tttttctact tcggcaacca
     4501 tattatcctg attggctggt gtaactatag tttttggaga ctttggtgtc tttaggttgg
     4561 gaacatgaat caacttactt tcttcgttaa aagaattggc caacatatag gttttctttc
     4621 ttaaaaattc agaataatcc tcaatggttt tatgttcatt ttgttctgct accgaaattg
     4681 gtttgcctac acgaacttta atcactctat ccttttgagt caacaattcc gatggcaatt
     4741 ttgccgtgcg aagttttgga tttaatttgg ataagaagta aaagaaccta ctgttttttg
     4801 catgaaaata aattggaact accggaacct gcgcttttcg gatgacttta attgcgcctt
     4861 cttcccatgc tttgtctact acaaatttac cgtctttata agtagaaact tcaccagcag
     4921 gaaacattcc taagggtttg ccatcgctca aatgacgcaa agtttcttta atgccaataa
     4981 cacttgattt tgcatctttt cggtcttcaa aaggatttac cggcatgatg tattgtttca
     5041 ttggttcaat gcgatgtaat aagaaattag caataatttt aaaatttggc tctcgttcta
     5101 gcattagttt taaaagcaaa atcccatcaa ttcctcctaa tggatgattg gaaatggtaa
     5161 tatatgcacc gtcttttggt aaacgtttta aatcttcttc aggaatttca aattttattt
     5221 gaaactcatc caaaatagca tttaaaaact ctacttcgct caaatgctta tttctatcat
     5281 agacgtcgtt taaggtagaa attcggagtg ctttcattaa tagccaacca ctaaacgtac
     5341 cgaaaactcc gtacttgtcc atatttattg ctttggcaac ttctttagct gtaactaaac
     5401 ccatgtagta gtattaaatt ttgagcaaaa aacaaagata acaattctct tcaaaaattt
     5461 tcttttaatt ttagaaaaga cataaaatct attgctcata agtttgttaa aaaagttcgc
     5521 tattttaacg aagtctttag atatttttaa agaaatttac acctatgaaa tcgcaattag
     5581 caatagaagt tgcaaataaa tagattagaa tggcgatacc atatgttacc gcttaaaaat
     5641 aaaatcaaaa catatgaaaa ttatttctta taacgtaaac ggaattagag cagcaataaa
     5701 caaagggttt gttgaatggc tagagcaagc caacccagat gtaatttgtt tgcaagaaat
     5761 taaagccacc gaagatcagg tgccagtgaa tgaactgaca caagcaggtt atccttatca
     5821 atattattat tcagcccaaa aaaaagggta tagtggcgtg gctattttat caaaaataaa
     5881 accaaatcac gtagaatacg gtactggagt tgaccacatg gattttgagg gtagaaattt
     5941 aagagtagat tttgatgaac tatcagtaat gagtttgtat ttgccttcgg gaacaaacat
     6001 tgaccgattg gatcataagt ttaagtacat ggatgatttt cagcactata ttcttagctt
     6061 acgaaatacc atacctaacc tagttatttg tggcgattat aacatttgtc atgaggctat
     6121 tgatatacat gatccagtta gaaacaaaac ggtttcgggt tttcttcctg aagaacgcgc
     6181 ttggttggat ggatttatga aaacaggtat gattgattct tttcgacatt ttaataaaga
     6241 gccaaatcat tatagttggt ggagttatcg tgccaatgct agaaacaaca ataaaggttg
     6301 gaggattgac tattgtttgg tggcacaacc actagaaaac aggttgaaac gagccgtaat
     6361 tttacccgaa gcaaagcatt ccgaccattg tcctgttttg gttgaattaa cttgaaaccc
     6421 caaatcacta actataaaat taaaaaaaat gattaaaaaa acattattaa ctggattgct
     6481 tttgttgacc ttatcatcgt gtgtttccaa aaaaatatac aatgatttag aaaacaaata
     6541 caccgatttg aagaaagaaa ataataacat ggcggatgaa ttagaaagtt tgcgtaaaac
     6601 aaaaaccgaa tttgaaaccg aaaacgcttc tttaaagtca gaattagcca aactaaaagc
     6661 ggaacgcgac aaactgcaaa ccgattgtac ggcaacagct aataatctaa aagctttaaa
     6721 agaatcgtat aatgctctag agaaaaatag tagtgaagca ttgacttcta acatgaacaa
     6781 aaatagagaa ttgttagcac aattggatac taaagagaaa gcactagccg ccgaacaaaa
     6841 tcgtttgaat aaattaagag atgaactttc tgcaagttca aagcgtttga gcgaattgga
     6901 aagcatgata gctaacaaag atgctgccat gaagaagcta aaagaaacgt tatcaaaagc
     6961 tttaaatgcc tttgaaggaa aaggattaac cgttgaacaa aaaaacggaa aagtatatgt
     7021 ttctatggaa aacaaattgt tgttccaaac tggaagttgg gctgttggtg ttgaaggaaa
     7081 aaaagcagta gttgaagtag gaaaagtgtt ggctcaaaat ccagacatta cggttttgat
     7141 tgaaggccat accgacaacg acaaaattct aggaacactg ggtggcggaa tagaaaacaa
     7201 ctgggattta tcgactaagc gtgctacagc aattgtaaac attttagcag aaaatgcagg
     7261 aattcaaaag aaaaatttaa cagcagcggg tcgtggcgaa tttgcacctt taatgagtaa
     7321 tgataccgct gaaggaaagg caaaaaaccg tagaattgaa attattttaa cgccaaaatt
     7381 ggatgaaatt tctaaaatgt tgaacgattt ttaagaaaaa atagtattta aaaaaaggtt
     7441 tagcaacgct aaaccttttt tgttttgttt tctttcagaa atgatttttt tcgaaaataa
     7501 tcttccattt tttattattt ggtagcaata ccgcaactct tgggttccat cccccaactc
     7561 tcgggcagca acccaaaaga aggtttttat aaccctttat gttgttaatt ccacccgcca
     7621 cctagggctt tgtagatatt ggttactgct attaattgat ttttcttggt ttctatcaat
     7681 tctaatttag actccaaagc atcgcgttgc gtcatcaata cttcaaaata atcaactctt
     7741 gacgatttga ataaatcatt agaaatagca attgcagtag ttaacgcttg cacttctttg
     7801 ttttttagag tataaaattg ctctaaatta gaaatatttg acaattcgtt tgacacttca
     7861 acataaccgt ttaagatgct tttttgatac tcatacatgg cttgaagttg catcgcattg
     7921 gctttgttaa attcagcttt tatagcattt ctattaatca aaggtgctgt taaatcgccc
     7981 actaaattat aaatcaatga ctcgggagtg gtaaaaagat aggaagtttt aaacgcatta
     8041 aacccaacac cacccgaaat agtaaacgat ggataaaact cggcttgcgc agcttttaca
     8101 tcacattttg cggcaaacaa ttccaattcg gcttgtttaa tatccggacg attgatcaac
     8161 aattgcgaag gaattccggc ttgtatttgt tgaggaatct cagccataaa actggttttg
     8221 tctctaacaa ttggttgagg aaaacgcgct aataagaaat tgattttatt ttcattttct
     8281 ttaatctgtt gcagaatttc atattctcta ctttgcgaat tcaacacttc ggcttcaaac
     8341 ttttttaccg ccagctccgt tactcgtgca gcctcttttt gcacttttac aatctctaaa
     8401 gcattttttt gcaaagcaat tgtttctttc acaatatcca attgattgtc taaagcgacc
     8461 aactcataat aggaattggc tatttcggca accaagtttg ttactacaaa attcttgcct
     8521 tctaccgttc ctaaatatct agaataggct gcttttttgg agttgcgcag ttttttccaa
     8581 atatccaatt cccaagttgc ttttagccca agcataaaat cttgcaatac ttcgggcact
     8641 tcatttccag gggttatctc ggtagaggca tcaccagcac cagtactcgt gtagcgacct
     8701 actttatcaa caccagccgc tgcaactgca tttacgctgg gtagcaacaa tccttttttt
     8761 acccgaacat catttcgggc gatttctatt tcttgcaacg ttaccgctaa ttcgatattg
     8821 ttttttaagg caatatcaat tagattgtct aagtttttat ccttaaaaaa atctttccat
     8881 ttaatttttg ccgaattcag cgtgtctttt gaaacagcat acgattctgg tactggttgt
     8941 ttttctatag ctgaaacggg tgctaacatt ttgcaacttg taaaaaagag gcaaaccaat
     9001 gctagtgcta ctattttatt ttttttaatg tatttcatta gagttcattt cttcggttat
     9061 aggattttct tcttcatctt caacgagtag gtgtttttca gaaattttgg cgaaaaggaa
     9121 atacaatcct ggaataagaa ttacaccaaa aacagttccg aatatcatgc cacctgctgc
     9181 tgctgcacca atagttcggt ttccaacggc tccaggtcca gtagcaaaaa ccaacggaat
     9241 tagtccagct atgaacgcaa aagaagtcat caaaattggt cgcaaacgag cagttgctcc
     9301 ttgtattgcc gcttttttgg cagacaagcc agcagcatgc ctctggacgg caaactcaac
     9361 tatcaatacg gcgttctttc ctaaaattcc aataagcatt accatcgaaa tttgtgcata
     9421 aatattgttt tctaacccaa gaacattgag caaaaagaat gcaccaaaaa ttccgaccgg
     9481 aagcgataaa atgatgggta atggcagcat gaagctttca tattgtgccg ataaaaccaa
     9541 atagacaaag actaaggaaa ttaagaaaat ataaatggct tgattccctt gtcgagattc
     9601 atcaatagag attccagccc attcaatccc gaaacctttg ggtaatgatt ttgcagctac
     9661 atctgcaatt acttttaaag ccgttccgct actgtaacct gcggctgggc caccactaat
     9721 ttcggcagcg ttgtataaat tgtgacgtgt tatttcggac aatccatata ctttttcaac
     9781 agtcatgaat gcgccataag gcaccatttc tccttcttcg tttttggtat atagtcttaa
     9841 gatgtcttcg ggaacagccc tatattgagg caaggcttgt accattactt tatattggcg
     9901 gtcgtattta ataaaactcg tttcatacgt actaccaatt aaaatcgata aattatccat
     9961 agcatcatca atagaaactc ctttttgttg tgctttatcg ttatcaactt ttaacatgta
    10021 ttgtggaaaa ctagcactat aaaaagagaa taccgaagta agttctggtc gtttagacag
    10081 ttcttttaca aaattcttag tgacactttc cattttttta taatcgccac taccaccttt
    10141 atctactact cgaagttcaa atcccccggc tgcaccataa cctggaacgg ctggaggtgg
    10201 gaaaaactca atactagcgc ctttgatgtt tttggatttt tcttccagct catccataat
    10261 ttgttgtgta ttgtgttttc tgtcgttcca gtcttttaga ttgattaaac aactagccga
    10321 gttggagcct gttccttccg acaatatttc gtatcctgcc atggtagaaa ccgatttcac
    10381 ttcatccatt tttgaagcaa tttcttgaag ttcatcagct actttgtcgg ttcgttctaa
    10441 ggttgaacca ggtggtgttt gaataatggc ataaaacaat ccttgatctt cattgggaat
    10501 aaaacccgaa ggaacgccag tacttaaacc ccaagttccc aaacaaaaag caactagaac
    10561 tagaatggtt ataactctac ggtttacaat agcactaact agtttttgat acttagtttg
    10621 gagaccattg aaccaattat tgaatctcgc taaaagagtg tttaaaacag tcttcttttt
    10681 ttcttgacca tgattgtttt ttagcatcat ggcacacaaa gcaggcgtaa atgtaagtgc
    10741 aacaactcca gaaagaataa ttgctgttgc catggttacc gagaactggc ggtaaaaaat
    10801 acccactggt cccgacatga aggaaacagg aataaaaacg gcagccataa ctaaggttat
    10861 tgcaataata gctcctgcaa tttctttcat tgccattttt gtagcttcta gtgcatcaac
    10921 attattttct tccatcttgg catgcactgc ttcgacgacg actatggcat tatcgactac
    10981 aattccaatg gctaatacca aggcgaaaag tgtaattaaa ttaatggtta tgccaaaaag
    11041 ttgcatgaaa aagaaagttc caactaatga aattggcaca gcaataatgg ttataaaagt
    11101 agaacgccaa tcgcctagaa agataaaaac gactaatgat actaataatg tagcttcaat
    11161 taatgtgtga attacttttt ctatagaagc atccaaaaat tttgaaacgt catatccaat
    11221 ttcataatcc attccttttg ggaatgattc cgccttaatt tcttctaatt ttttctttac
    11281 attttcaatt actttacttg cgttacttcc ataggattgt ttaataacaa tagctgctga
    11341 aggttttccg tttaatttgg aatataaatt ataaaattca tttccaaatt caatttgggc
    11401 aacatctttt aatcgcaaca attctccatt tggattagaa cgaattacta tattttcata
    11461 ttgttcttgg gtggtatatc taccgggata tttgatgaca tattcaaaag cttgggattt
    11521 tttacctgaa ctttctccta attttcctgg tgaagcctca aaactttgac tttctaatga
    11581 tttcatcact tcttcggatg aaactttgta cgccaacatt ctgtcgggtt ttaaccaaat
    11641 acgcatcgaa tattctctat cgccaagaac atcggcaact ccaacaccat caacacgttt
    11701 taattcagaa agcaggttta tatcggcata gttgtataaa aagtttacat caagatttgg
    11761 atctttactg tataaattaa tatacatcaa catattggat tcttcacgtg tgatttttac
    11821 accttcacga acaacaattg gcggcaattt atttacaaca gctgccacac gattttgaac
    11881 attcacagaa gcgatattag gatcggttcc taactcaaat acaacttgta tcgaagcttc
    11941 accatcattt ccagcatcag aagaaatata tttcattcca gggattccat ttatggctct
    12001 ttctaacgga attacaactg atttaatcat taattcgcca ttggcaccag gatactcggc
    12061 aacaatattc acttttggtg gtgaaataga cggaaattga gtaataggta attgtaccaa
    12121 agccaaaaat cctaaaaaca aaataattaa tgagattaca atagacaata ctggtctttg
    12181 tatgaattta ttaaacatag caacgaggtt ttagattgtt tgtatgaact attgttgaat
    12241 taactgtaaa tccttaagaa ttttatctgg agatacttct tttggaataa tcttttcgtc
    12301 ttctttaacc atttgaattc cttcaactaa gattctttct ttctctgaaa ttcccgatgc
    12361 tactatataa atatttgaca agcgttgctt aactataatg ttttgtgaat gtaccttgtt
    12421 attcacatca actacataaa cataaatttt atcttgaact tcaaaagtgg ctttttgagg
    12481 aataatcaaa gcgtttttaa cgggtgttgt catcaaaact ttacccgttt caccgtgttt
    12541 taacaaataa tcaggatttg gaaatttagc tctaaaagca atgtttccag tattattgtc
    12601 aaattcgctt tctactgttt caataacacc tttgtattta tgcaaatcat tatttgaaag
    12661 taataatgaa actgaatttg taatatcatt ttttggtctt gatttatact ctaaatattc
    12721 aggttcggac atgttgaaat aggcaaaaat atctttgttg ttagaaatag tagtcaaaac
    12781 cgttccttcg tcaatcaaac ttcctttttt gaaacgaatt cggtctatag ttccatcaaa
    12841 tggtgctttg attttggtaa atgacaaatg tgtttcagcc atttctaatt cggcattggc
    12901 ttgttcaagt tttgcttgtg ccatggccaa ctcagatttt gaaacaatat ttttgtcagc
    12961 taaagttttg gtgttttgta attctaatgc ggcaatttta gcttcagctt tagcttttaa
    13021 ataatcggct tcataaagct ttggcatgat ggtaaatagc aattgacctg cttttacatt
    13081 gcttccttca tccacatata agttttctaa atagcctttt tcttgtgctc taagctctac
    13141 attttgaata gattgtattt gagcaatata ttctttggcg tatgttgtgt cttttacaat
    13201 tgggttggta acaatatatt cagctacttc tttattttct ttttctttgg ttgtgcaact
    13261 tgacaacagc gacaatgcac aaaaactgat tagtaagact aacttgttca tgtatttata
    13321 taatataatt tttaatacta aaattttgat tgatttatgt ctaaaatcaa taactcataa
    13381 gtaattaaaa ttatatcaca atcgtaaaac ttggagaatg ataaatgtaa accgcggtaa
    13441 tcttgaagaa tttatcgaaa aatagccttt tcgaggcgat tttaaacaaa taaagtttgt
    13501 gtaattagaa gtttttttta aagcggttac gccaattaca gtattttttt tgccgatgga
    13561 tgagttgaaa ttttcactat catcattatc ataattgtaa agtatagtat cgctatttgg
    13621 cgatagataa tttacagatt tagttatttg attagaattt acaaaactat ttatggaatt
    13681 tgaaactaag actgaattag ttgcaaatga gaaagtgtgg cttgttacca aaagaaacaa
    13741 acccaaagta aaaaaatatg taactatttt tttcataatt cactgcaaat ataattcgca
    13801 ttgaaataga aaatacttct ttaagatttt gtgtaatcaa tttaagcaaa tctttagaat
    13861 gtatttaaaa gtat
//
LOCUS       DAOOBH010000054        13789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5736_length_13789_cov_5.488059, whole genome shotgun sequence.
ACCESSION   DAOOBH010000054 DAOOBH010000000
VERSION     DAOOBH010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5736_length_13789_cov_5.488059"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PK218_06855"
     CDS             <1..1023
                     /locus_tag="PK218_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPW98259.1"
                     /translation="FLCLIGLTACKTSKEYVSKEFTSITIDTLYTSKISIRAIASDND
                     TIYFGGSDKKIGFVSAKEKYEKQLTKLPYIFEFRSIALTSKYVYFLSIDNPALLYRYN
                     RNLKQSELVYEEKHEKVFYDSMHFWNDEEGIAIGDPTEDCLSIIITRDSGKTWNKIRC
                     VQLPKVINGEGAFAASNTNICIKGNSCWVVSGGKQSRVFYSKDKGISWEVFDTPIVQG
                     EAMTGIFTADFYNEKIGFIAGGNYEMPNQNFQNKAITFDGGKTWKLVGEKVGFGYASC
                     VQFVPNSKGKALVSVGATGLFYSNDFGNSWKQFTTDNSLFTIRFVSPTKAIAAGKDKI
                     ISIVFQ"
     gene            complement(1055..1507)
                     /locus_tag="PK218_06860"
     CDS             complement(1055..1507)
                     /locus_tag="PK218_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor of ECF-type sigma factor"
                     /protein_id="HPW98260.1"
                     /translation="MKTKQLLLTLLCLVCVTAFSQNRLKEKQNQIKSIKVAFITNELS
                     LTPDEAAKFWPIYNAFEDKQLEIRKTKLKGYISRMDEDSLNTLTEKEATTMLTQIESA
                     EEDLFLLKKKFNADIRAVLPASKIVKLKKAEEKFNRKLLQQFKEKRNK"
     gene            complement(1509..1925)
                     /locus_tag="PK218_06865"
     CDS             complement(1509..1925)
                     /locus_tag="PK218_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98261.1"
                     /translation="MNEFKLDNQPKISPGFKVPEGYFDTLSENINARLNADEPKVISL
                     YQRHKKTIYAVAAVLAIALSVPFFMQQQPKVLSDLDQSVIEDYISYNTKMTPYELAEY
                     LDKEDIEKLKVDFEIQDKVLEESLLNNIDVEQYIVN"
     gene            complement(1956..2498)
                     /locus_tag="PK218_06870"
     CDS             complement(1956..2498)
                     /locus_tag="PK218_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPW98262.1"
                     /translation="MQDEKVFIEELLNPKTQNKAFQVLLQQFQKPLYHHIRTIVLNHD
                     DADDVLQNTFVKVFRYLKDFKGDSKLFSWMFRIATNEAITFLNQKAKKHNTTGVAIQT
                     KTIDNLEADDYFDGDEIQLKLQKAIVLLPEKQQLVFKMKYFEELKYEEISEILGTSVG
                     GLKASYHHAVKKIEEYMNIH"
     gene            complement(2559..3680)
                     /locus_tag="PK218_06875"
     CDS             complement(2559..3680)
                     /locus_tag="PK218_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98263.1"
                     /translation="MRIIKYTFLLLLLFSIGLFVYIFTQNGNYKITRSQIIKASRYTV
                     FEYVNDYKNWETFHSMKQRLSGVTFDYKAQTSGKGGSFSWKSDSDNGFMKTNFVKDND
                     SIAQKMVSDGYNATINWKFKDTIGGTKVTYSTKGSIGVLQKFSTFFQGGIKSILNNDI
                     DRTLYNLNKTLDFELKTYSVKVNGVAQRNAAYFIKQSFTCKTKNLSRNIKIVMPKLVY
                     FFKKNNIPMAGKPFVLYETTTLKTDEVTVSVCIPTQQLVALMEGSDVSSGEMEAFTCI
                     KTTLKGDYSHRDALWKKAQDYILEKDFKPNFAGKYIERYTKTIDDVKNPSKWETELYI
                     PVFPKVVPTVVTATTVSVPPISSENQPVHSTSEASQEIP"
     gene            3777..5396
                     /locus_tag="PK218_06880"
     CDS             3777..5396
                     /locus_tag="PK218_06880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW98264.1"
                     /translation="MLTTQKKSELRAVLFQHLDGIVTAPVAFVLHQKGVLSYVFEKQK
                     VTLHELTEKFEANDGYLNVGLRVLASQGFLKQQLDNTNDEVIYSINDRSEEAFSHFYL
                     YEDVFKLIQFSEKFHPRKFEVAPFEMLNSCFKKYKQQYGITFSDDAQTKAIQHQILKH
                     IEGNLVGPTIVMLGMSGMFHKYFMETSFRPEEFHKNPECFKEILDFLTFLGWFKEKDG
                     KYQFTEMGLFYAKRAAAYGVTVSYIPMFRKMEDLLFGNAAILRNIGEHEDELHVDREM
                     NVWGSGGAHAEYFKVVDEIIIELFNRPINEQPKGILDMGCGNGAFIEHIFDVIERQTA
                     RGKILDDYPLFLIGVDYNEAALKVTRANLIKADIWAKVIWGDIGKPDVLATTLQEDYD
                     IDLKELLNVRTFLDHNRIWEQPKHRTPNRVSTSSGAFAHRGKRITNNEVEDNLLEHLK
                     KWSVYVEKFGLLNIELHTISPELTAKNLGRTAATAYDATHGFSDQYIVEIEVLHKVAA
                     EAGLFSSQDFFTKFPNSDLATVSVNLLKGKN"
     gene            complement(5393..5581)
                     /locus_tag="PK218_06885"
     CDS             complement(5393..5581)
                     /locus_tag="PK218_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98265.1"
                     /translation="MFTTGQWVFAALFFVAFVIAAYFAYGKDKALHQKQFKGSYKILI
                     GFLLFVVMLFVIKLVMKR"
     gene            5658..6041
                     /locus_tag="PK218_06890"
     CDS             5658..6041
                     /locus_tag="PK218_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HPW98266.1"
                     /translation="MQKQLNAVKEFHTAFGLGVSHEMKADLGEQKNMLRFNLMKEENE
                     EYLDAVQNNDLVETADALGDMLYILCGTMLEHGLQHKIEEVFDEIQRSNMSKLGEDGK
                     PIYREDGKVMKGPNYFKPNFETILK"
     gene            complement(6050..6982)
                     /locus_tag="PK218_06895"
     CDS             complement(6050..6982)
                     /locus_tag="PK218_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98267.1"
                     /translation="MKKFIIEIFKFTLFAIIFYIITLLIWESIKYPFLKPNLYCNKGS
                     YGNTFTRLKEAKKINTVDVLILGSSHAYRGFDTRIFNKNNISSFNLGTSSQTPIQTLA
                     LLKRYLKTIHPKLIIVEVYPGVLNSDGVESSLDIINNDENDFLSLEMVLKQNNIKTFN
                     TLIFNYYRNFLNLDNSFVEKRIKNDDTYISGGYIEKKIGYYKPKNIAKQPIIIDKKQL
                     DAFEDIIYEIKNRNIELVLVYAPISKNLYSSYTNNHYFDGLMMKYSEYYNFNSMIKLN
                     DTIDFDDSNHLNQIGVEKFNNKLIEIIKSRFSKN"
     gene            complement(6992..8443)
                     /locus_tag="PK218_06900"
     CDS             complement(6992..8443)
                     /locus_tag="PK218_06900"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPW98268.1"
                     /translation="MLFNSLDFAVFLPSVFFLYWFVFNKNLKHQNLLIVVSSYYFYGL
                     WDWRFLFLIFFSTLVDYSIGKSLLNVTNQKKRKGLLLISVFVNLGLLVFFKYYNFFLD
                     NFIATFKFFGHPIDIQGLNIILPVGISFYTFQTMSYTIDVYRKKIEPTDNFITFCAFV
                     SFFPQLVAGPIERASHLLPQFTKKRTLEYDKAVDGMKQILWGLFKKMVIADNCALFAN
                     QIFDNSSELNGSTLLLGAVFFAFQIYCDFSGYSDIAIGVARLFGFDLMRNFAFPYFSR
                     DVAEFWRRWHISLSTWFRDYLYIPLGGSRGSNWMKIRNIFIIFLVSGFWHGANWTFLV
                     WGALNAFYFLPLLLSKKNRTNIDIIAQGKFLPSFKDFLAMVFTFFITTIAWVFFRANT
                     IQHAGSYVSGIFSSSLFDMPHFKEINKGFTVILLLLFFIFIEWCGRESQFAIENIGVR
                     WPRIIRWSFYSFLIFCIGMFMATYETPFIYFQF"
     gene            8722..9393
                     /locus_tag="PK218_06905"
     CDS             8722..9393
                     /locus_tag="PK218_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW98269.1"
                     /translation="MTHKLLLVEDETGIMQFLKQGLEEEGFIITTANNGAEGLALALN
                     NKFDLLLLDWMLPKMTGYQLCIAFRETNKKTPIIFLTAKDTVQETIEGLKAGANDYIK
                     KPFNFDELLERIKVQLRNKELPQLLTLGNVSIDLQKHIVTVNDMEVSLTQREFELLAY
                     LVKNKGKVCTRTEIIQDVWDIHFEYDTGVIDVFMNAIRKKLNLKVEEDYIKTVRGVGY
                     IANDN"
     gene            9393..10775
                     /locus_tag="PK218_06910"
     CDS             9393..10775
                     /locus_tag="PK218_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW98270.1"
                     /translation="MKPLSFKNRIAFNYIITTALLIFVVFFAIFSIVKYSVYVRVNNE
                     IKEEVAKHLKEIEVVENCVLLIREEQWKLKEFNRLDASPAFIQFVDELGTLVEKSPNL
                     KQKQLHFYPKVKENKLFDAKLDNISVRQTQIPLYQGTKIIGYLVVAMSLEDSVMVLNN
                     LFIIMLIAFPVILIILFLIARFIAGKSIKPINSIINTANFITKDNLESRIPLPQNKDE
                     LYILSQTINNLLERIENAVIREKQFTSDASHELRTPLAVIKGTLEVLVRKPRTEEEYK
                     EKIGYCVSEVNRLNHLVDELLLLARFENQKQNQKLEQVNIVSVVSDTLARNSAHIKDK
                     KLNIITDYSNEVNAKTDSYLFALIVNNILTNAIKYSNNSTDITISIEEKNDLVICSIA
                     DNGIGIAKEEINKIFDQFYRSKSNEHPEIKGTGLGLSIVRRLCDLLEINLNINSEENK
                     GTTVYLTLKK"
     gene            10778..11287
                     /locus_tag="PK218_06915"
     CDS             10778..11287
                     /locus_tag="PK218_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98271.1"
                     /translation="MTDAHLHLVVNHFPIIGLFFAIGIMVFGVFLKSNILKNTAYVML
                     IITAIFGFLSMYTGEGAEEMVEDMPNIGHKIIHEHEELAEKFVLFLYAAGLLSILGLY
                     FNLKMHSKANIVAYATLVVAIIAGIFAVQVGTSGGEVRHTEIRTEKVAQNNDIEKNYS
                     DEHTDHDNN"
     gene            11982..12524
                     /locus_tag="PK218_06920"
     CDS             11982..12524
                     /locus_tag="PK218_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98272.1"
                     /translation="MKSYNQKNFHKYTFCLWQEIPFTEIQSLFINHKSKSGSQYIFTT
                     KGVYRISNHWGRAANCRWRLQPLENYKNQQIKVGFAHWTDFYPNNEIENLFFIQVNFN
                     TNEVTFNHKAAPAYNKTYTLRNAAQTSKIIKLIKQILTETTWAKHLNYNNIDTLRREI
                     INKLLTTKSSFFDIKKQFTI"
     gene            12709..13581
                     /locus_tag="PK218_06925"
     CDS             12709..13581
                     /locus_tag="PK218_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPW98273.1"
                     /translation="MINNFLKVNLETKRLMNTNVVENYSKLKLSLFVLPLFLLLSIVL
                     FLISVDALSVNGYVEIQKECFIFLNSEFSQFPNLIYNLTQIGDALIFLSLISVFIVYA
                     PKIWESLITASLVSAVFSRILKDIFSIPRPAEVFDNSIFVIVGRRLPGFSSLPSGHSI
                     TVFTGLTVLLFAFMPKKWVDKILWCFFILVLGYSIAFTRVGVGAHHPLDVISGSIIGY
                     ISALLGIFISRKYKIWAWINNIKSYPFFILLFSICCFVLIHKIYKENLVIFYLALTSL
                     IVSLYKIIYVYIKK"
BASE COUNT         4880 a         2158 c         2154 g         4597 t
ORIGIN      
        1 ttcctttgtc taattggttt aacggcttgt aaaacttcta aagaatatgt gtcaaaagaa
       61 tttacttcta taacaattga tacactttat acaagtaaaa taagtattcg agccattgct
      121 tctgataacg atacaatcta ttttggtggt tccgataaaa aaataggatt tgtgagtgca
      181 aaagaaaaat atgaaaaaca actcactaag ttgccctata tctttgaatt ccgaagcatt
      241 gctctaacat caaagtatgt ttacttctta tccattgata atccagcgtt attataccga
      301 tataatagaa acctcaaaca aagtgaattg gtttatgaag agaaacacga aaaagtgttc
      361 tacgacagta tgcatttttg gaacgacgaa gaaggaatag ctattggcga tccaacggaa
      421 gactgtttaa gcataatcat tactcgagat tctggaaaaa cttggaacaa aatacggtgt
      481 gttcaattgc ccaaagtaat aaatggcgaa ggagcatttg cagctagtaa taccaatatt
      541 tgtatcaaag gcaattcgtg ttgggtagtt tctggcggaa aacaatcaag agtgttttat
      601 tcaaaagata aagggataag ttgggaagta ttcgatacgc ctattgttca aggtgaagcc
      661 atgactggaa tattcactgc cgatttttat aacgaaaaaa taggatttat agctggaggc
      721 aattatgaaa tgccaaacca aaattttcaa aacaaagcca ttacatttga tggcgggaaa
      781 acatggaaat tagtaggcga gaaggttggc tttggttatg cttcctgcgt acagtttgtt
      841 cccaattcca aaggaaaagc cttggtgtca gtaggtgcta cgggattgtt ttattctaat
      901 gattttggaa attcttggaa acaatttaca actgataata gtttgtttac catccgtttt
      961 gtttcaccaa ccaaagccat cgctgcagga aaagataaaa ttatcagtat cgtttttcaa
     1021 taaaaaaaaa gaacattgct gctcttttct tttattattt atttcttttt tccttaaact
     1081 gttgcaacag ctttcggttg aatttttctt cggctttttt tagttttact atttttgaag
     1141 caggcaatac agcacgtatg tcagcgttaa acttcttttt caataaaaac aaatcttcct
     1201 cagcactttc tatttgtgtg agcatagtag tagcttcttt ttcagttaag gtatttagag
     1261 aatcttcatc cattctgcta atgtagcctt tcaactttgt ttttctaatt tcaagttgtt
     1321 tgtcttcaaa agcattatag attggccaaa attttgcagc ttcatctgga gttaatgaca
     1381 attcgttggt aataaaagct actttaatcg atttgatttg gttttgtttt tctttcaatc
     1441 tattttgact gaacgcggta acacacacca agcacaatag cgttagtaat agttgttttg
     1501 ttttcatatt aatttacaat atattgttct acatctatgt tgtttaaaag cgattcttcc
     1561 aaaaccttgt cttgaatttc aaaatctact ttaagttttt ctatatcttc tttgtccaaa
     1621 tattccgcta gttcataagg tgtcattttg gtgttgtaag atatatagtc ttcaatgaca
     1681 ctttggtcta aatcggatag cactttaggt tgttgttgca taaaaaaagg aacactcaat
     1741 gctatagcta aaaccgctgc cactgcataa atagttttct tatgacgctg atataatgaa
     1801 ataactttgg gttcatcagc attcagtctt gcattgatgt tttcagataa tgtatcaaaa
     1861 tatccttctg gcactttaaa accaggagat attttgggtt gattgtctaa tttaaattca
     1921 ttcatactac tttgacttaa attctactaa aaggtttaat ggatattcat atattcttca
     1981 attttcttca cggcatgatg ataagaagct ttcaagccac caaccgaagt gcctagtatt
     2041 tctgaaattt cctcatattt caattcttca aaatacttca tcttgaaaac taattgttgt
     2101 ttttcgggca aaagaacaat cgctttttga agtttaagtt gaatttcatc gccatcaaaa
     2161 taatcatcag cttcgaggtt atcaatagtt ttggtttgta ttgctactcc agtagtgtta
     2221 tgtttcttag ctttttgatt taaaaaagtt atggcttcat tagttgcaat tcgaaacatc
     2281 caagaaaata atttactgtc acctttaaaa tctttcaaat agcgaaagac ttttacaaac
     2341 gtattttgca atacatcatc cgcatcatca tggttcaaaa caatagtcct aatgtgatgg
     2401 tatagcggtt tttgaaactg ttgtagcaag acttgaaacg ctttgttctg cgttttagga
     2461 ttcaataatt cttcaataaa aaccttttcg tcttgcaaat ttgaaagttt gtaataagaa
     2521 taaaaataaa ccaaaaggtt taatcactac ctataaaatt atggaatttc ttggcttgct
     2581 tctgatgttg aatgaacggg ttggttttcg cttgatattg gcggtactga aactgtagtt
     2641 gcagtaacga cagttggcac tacttttgga aaaacaggga tatacaattc agtttcccat
     2701 tttgatgggt tttttacatc gtcaatcgtt ttggtgtatc gttctatata ttttcctgca
     2761 aaattaggtt taaagtcttt ttcaagaata taatcctgtg cttttttcca taaagcatca
     2821 cgatgcgaat aatcaccttt cagagttgtt ttaatacaag taaacgcttc catttcaccc
     2881 gatgacacat cacttccttc catcaaagca actaattgct gtgttggaat acataccgat
     2941 actgtaactt catcagtctt tagtgttgtt gtttcatata atacaaaagg ttttcctgcc
     3001 atcggaatgt tattcttttt aaagaaatag accaacttag gcatcactat ttttatgttt
     3061 cgcgacaagt tttttgtttt acacgtaaag ctttgcttga taaaataagc agcattgcgt
     3121 tgggcaacac catttacttt taccgaatag gtttttagtt caaaatcaag cgttttgtta
     3181 agattatata gtgttcggtc aatgtcatta ttcaagatgc ttttaatgcc gccttgaaag
     3241 aatgtactaa acttttgtaa cactccaatg cttcctttgg tagagtaagt tacttttgtg
     3301 ccaccaatag tatctttaaa cttccaattg attgttgcat tataaccatc agataccatt
     3361 ttttgtgcaa tactatcgtt gtcttttaca aaattggttt tcataaaacc gttatcacta
     3421 tcacttttcc aagagaaact gccgcctttg ccagaagttt gtgctttgta atcaaaggtt
     3481 actcccgaaa gacgttgctt catcgaatga aaggtttccc agttcttata atcgttaaca
     3541 tattcaaaaa cagtgtatct tgatgctttg attatttgag aacgagtaat tttataattg
     3601 ccattttgag taaaaatata aacaaacaat ccaatggaaa ataagagaag tagtaggaag
     3661 gtatatttaa tgatgcgcat aattctaaat aaagggttga acaaagttat aaatataatt
     3721 cgtctaatcg attagtattt aattatattt gttatatcat caattctttt ttagctatgc
     3781 ttactactca aaaaaaatct gaattaagag ctgttttgtt tcaacatctg gatggtattg
     3841 ttacagctcc agtggctttt gtactccatc aaaaaggtgt tttgagctat gtttttgaaa
     3901 aacaaaaagt aacacttcat gaacttaccg aaaaatttga ggcaaatgat ggctatctca
     3961 atgttggcct tcgtgttttg gcttcacaag gctttttaaa acaacagttg gacaacacaa
     4021 atgatgaagt catatattcc atcaatgata ggagtgaaga ggctttttcc catttctatt
     4081 tatatgaaga tgtttttaaa ttaattcaat tttcagaaaa gtttcatcca agaaagtttg
     4141 aagtagctcc ttttgaaatg ctcaattcat gttttaaaaa atacaagcag caatatggaa
     4201 taacattttc tgatgatgca caaaccaaag cgattcaaca tcaaattctt aaacacatcg
     4261 agggcaatct tgttggacca accatcgtaa tgttagggat gagtggtatg tttcacaaat
     4321 attttatgga aacatcattc cgtccagaag agtttcataa aaacccagaa tgttttaaag
     4381 aaatattaga tttcttaacg ttccttggtt ggtttaaaga aaaagacgga aagtatcaat
     4441 tcacagaaat gggattgttt tacgctaaaa gagctgccgc ttatggcgta acggtttctt
     4501 atattccaat gttccggaaa atggaagatc ttctttttgg aaacgcggcc atacttagaa
     4561 acattggcga acacgaagat gaattacacg ttgaccgcga aatgaatgtt tggggaagtg
     4621 gtggtgcgca tgccgaatat tttaaagtag ttgacgaaat tattattgaa ttattcaaca
     4681 ggccaataaa cgaacagcct aaagggattt tagacatggg ttgcggtaac ggagcattta
     4741 tagaacatat atttgatgta attgaacgac aaaccgccag aggaaaaata ctagatgact
     4801 atccgttgtt cctaattggc gttgattata acgaagctgc tttaaaagta acgcgcgcca
     4861 acttaatcaa agccgatatt tgggcaaaag taatttgggg tgatattgga aaacccgatg
     4921 ttttagctac aactttacaa gaagattacg atattgattt aaaagaattg ctgaatgtta
     4981 gaacttttct agaccacaat cgcatttggg aacaacctaa acacagaaca ccaaacagag
     5041 taagcacatc atcaggcgca tttgcgcatc gaggaaaacg aattaccaat aatgaagttg
     5101 aagacaatct gttagaacat ctgaaaaaat ggtcggttta tgtagaaaag tttggattgc
     5161 ttaatataga attgcataca atttcacctg aactaactgc caaaaactta ggaagaacag
     5221 ctgctacggc ttatgatgca acacatggtt tttcagacca atacatagta gagattgaag
     5281 tgctacataa agtagctgcc gaagcaggtt tgttttctag tcaagacttc tttaccaagt
     5341 ttcccaattc tgatttggca acagtgagtg tcaacttatt gaaaggtaaa aactaacgtt
     5401 tcattaccaa tttaatgacg aaaagcatca ctacaaacag tagaaatccg attaagattt
     5461 tataacttcc tttgaattgt ttttggtgaa gtgctttatc ttttccataa gcaaagtagg
     5521 cagctattac aaaagctaca aaaaacaaag cggcaaaaac ccattgacca gttgtgaaca
     5581 ttttttctaa attttcaaca aatttactca aatgaatcgg attgttgtat tttgcatcaa
     5641 gaataaaaca ccataatatg caaaaacaat tgaacgcagt aaaagaattt cacactgctt
     5701 ttggactagg agtaagtcat gaaatgaaag ccgatttagg agaacaaaaa aacatgcttc
     5761 gttttaattt gatgaaagaa gaaaacgaag aatacctcga tgcagttcaa aacaacgatt
     5821 tggtagaaac agccgacgcg cttggtgata tgctctacat tctttgtgga acgatgctcg
     5881 agcatggttt gcaacataaa atagaagagg tgtttgacga aattcaacga agtaacatga
     5941 gcaaacttgg cgaagacgga aaaccaatat accgagaaga cggaaaagtt atgaaaggac
     6001 caaattattt taaaccaaac tttgaaacga tattaaaata attcaaaaat taattctttg
     6061 aaaacctact ttttataatt tctataagtt tgttgttgaa tttttcgaca ccaatttgat
     6121 ttaaatgatt cgagtcatca aaatctatag tgtcatttaa ttttatcatt gaattaaagt
     6181 tgtaatattc agagtatttc atcattaaac catcaaaata atggttgttt gtgtaactag
     6241 aataaagatt ttttgaaatt ggggcataaa ccaatactaa ttcaatgttg cgatttttta
     6301 tttcgtaaat tatatcttca aaggcatcta attgcttttt gtcaataatt ataggttgtt
     6361 ttgcaatatt ctttggtttg taataaccta tttttttttc aatataacca cctgaaatat
     6421 aggtgtcatc atttttaatt cttttttcta caaaactgtt atctagattt aaaaaattcc
     6481 tataataatt aaaaatcaaa gtattaaaag tctttatgtt attttgtttt aataccattt
     6541 ctaatgataa aaaatcattt tcatcattat ttataatatc taaagaggat tcaactccat
     6601 ctgaatttaa tacaccagga tatacctcta ctatgattaa ttttggatgt attgttttta
     6661 agtagcgttt aagtagtgct aatgtctgaa taggcgtttg agagctggtg cctaaattaa
     6721 acgatgatat attattttta ttaaaaattc ttgtatcaaa tcctctgtag gcatgcgatg
     6781 aacctagaat gagaacatca acagtgttaa ttttttttgc ttcttttaat cgagtaaacg
     6841 tatttccata agatcctttg ttacaatata aatttggctt taaaaatgga tatttaatac
     6901 tttcccaaat tagtaaagta attatgtaga aaataattgc aaagagagtg aatttgaata
     6961 tttcaatgat aaattttttc atttttgcga tttaaaattg aaagtatata aacggagttt
     7021 cgtaggttgc cataaacatt ccaatacaga aaattaaaaa agagtaaaaa ctccatcgga
     7081 taattctagg ccatctaaca ccaatgtttt caatagcaaa ttgactttct cttccacacc
     7141 attcaataaa aataaaaaat aataaaagta aaataactgt aaaaccttta tttatttctt
     7201 taaaatgagg catatcaaat aagctagaag aaaaaattcc agacacgtaa cttccagcat
     7261 gttggatagt attggctcta aaaaacaccc aagctattgt tgttataaaa aaagtaaata
     7321 ccatagctaa aaaatcctta aaacttggta gaaatttccc ttgcgcaatg atgtctatat
     7381 tagttctatt tttttttgag agtaataatg gtaaaaagta gaaggcattc agtgctcccc
     7441 aaacaagaaa agtccaattt gcaccatgcc aaaaaccact tactaaaaag ataataaaaa
     7501 tatttcgaat tttcatccaa ttacttcctc tacttccgcc taaagggatg tataaataat
     7561 ctctaaacca tgttgataac gaaatatgcc aacgcctcca aaactcagcc acatctctag
     7621 aaaaataagg aaaagcaaaa ttacgcatca agtcaaaacc aaacaatcgc gccactccaa
     7681 ttgcaatatc tgaatatccc gaaaaatcac aataaatttg aaaggcaaaa aatacagctc
     7741 ccaaaagcaa agtgctgcca tttaattcag aagagttgtc aaaaatttga ttcgcaaata
     7801 gggcacagtt atccgcaatt accatttttt taaacaatcc ccaaagaatt tgtttcatgc
     7861 catctactgc tttgtcatat tctaaggttc tttttttagt aaactgagga agtaaatgtg
     7921 aagctctttc tattggtcca gctaccaatt gaggaaagaa actgacgaaa gcacaaaaag
     7981 ttataaagtt atccgtaggt tcgatttttt ttctataaac atctatcgta taactcattg
     8041 tttggaaagt ataaaaacta attccaaccg gaagtataat gtttaaaccc tgaatgtcta
     8101 taggatgacc aaagaattta aaggtagcaa tgaagttgtc aagaaaaaaa ttgtaatatt
     8161 tgaagaaaac aagaagtcct agattaacaa aaacacttat taaaagtaag cctttccgtt
     8221 ttttttgatt tgttacattt aacaaactct taccaataga ataatccact agtgtgctaa
     8281 aaaatatcaa aaacaaaaaa cgccaatccc acaaaccata aaagtagtag cttgatacaa
     8341 cgattaaaag attttgatgt tttaaatttt tattgaaaac aaaccaatat agaaaaaata
     8401 ctgatggtaa gaatactgca aaatcgagcg aattaaaaag catatattct gtttagtttt
     8461 ggtgattgtt tgttcacttt caggtaataa taatttattt caaaagtaaa ttaaatagct
     8521 aaattaataa atctatagat ggaaattcat tgatatttaa attttttatg ctttttacat
     8581 ttacaagagg ttctaacctt agataaaatc ttctaaaata ttaaatttaa tcaacagtac
     8641 attaaaattc acttaataat aaaacagacc gaaaaactaa aacccatttt gagtactttt
     8701 acatattatt tctaaaaact catgacacat aaactattac tagtcgaaga tgaaacaggt
     8761 atcatgcaat ttcttaaaca aggattagaa gaagaaggtt ttatcattac cactgccaat
     8821 aatggtgctg aaggtctagc acttgctcta aataataaat tcgatttgct actattagat
     8881 tggatgttgc caaaaatgac gggttaccaa ctttgtattg cctttagaga gacaaacaaa
     8941 aaaactccaa ttattttttt aaccgcaaaa gatactgttc aagaaaccat agaaggactt
     9001 aaagcgggtg ccaacgatta tataaaaaaa ccgtttaact ttgatgaatt attagaacgc
     9061 attaaagtac agcttcgtaa taaagagctg ccacaattat taacattagg taatgtttct
     9121 attgatttac aaaaacatat tgtaacggtc aacgatatgg aagtgtcact aacacagcga
     9181 gagtttgaat tattagccta tttggttaaa aataaaggaa aagtttgcac tcgaactgaa
     9241 attattcaag atgtttggga cattcatttt gaatacgata cgggcgtaat tgatgtattt
     9301 atgaatgcca tccggaaaaa attaaatcta aaagtagaag aagattatat caaaaccgtt
     9361 cgcggtgttg gttatattgc aaatgacaat taatgaaacc actttcgttt aaaaacagaa
     9421 tagcattcaa ctatatcatt actacagcat tattaatatt tgtagtgttt tttgctattt
     9481 tttctattgt aaaatacagt gtttatgtta gagtgaataa cgaaattaaa gaagaagttg
     9541 ccaaacattt aaaagaaatt gaagtcgttg aaaactgtgt acttcttatt cgagaagagc
     9601 aatggaagct taaagaattc aatagattgg atgccagtcc agcgttcatt caatttgttg
     9661 atgaacttgg aacattagtc gaaaaatcac ctaatttaaa acaaaaacaa ttacacttct
     9721 acccaaaagt aaaagaaaac aaactttttg atgccaaatt ggataatatt tctgttcgtc
     9781 aaactcaaat accactatac caaggaacaa aaataattgg atatttagtt gtagccatgt
     9841 ctttagaaga ttctgtgatg gtactcaaca acttgttcat catcatgctg attgcctttc
     9901 cggtaatctt aatcattctc tttttgatag cacgttttat tgctggaaaa agcattaaac
     9961 ctatcaattc gattatcaac acagcaaatt ttattaccaa agacaatcta gaatctagaa
    10021 ttcctttgcc tcaaaacaaa gacgaacttt atattctttc acaaacgata aacaatcttt
    10081 tagaacgaat tgaaaacgca gtaattcgtg aaaaacaatt cacttcagat gcttcacacg
    10141 aacttagaac gccattagct gtaattaaag gaacacttga agtgcttgtt cgtaaaccca
    10201 gaacagaaga agaatacaaa gaaaaaattg gttattgtgt aagtgaagta aatcgtctaa
    10261 atcatttagt agatgaatta ttattactcg ctcgttttga aaaccaaaaa caaaatcaaa
    10321 agttagaaca agtcaatata gtttcagtgg tttctgatac tttggctaga aactctgcac
    10381 acattaaaga caaaaaatta aatataataa cggattattc aaatgaagta aatgccaaaa
    10441 ccgacagcta cttatttgcg ttaatcgtca ataatatctt gactaatgct ataaaatatt
    10501 ccaataatag cactgatata actatctcga ttgaagaaaa aaatgatttg gtaatttgct
    10561 ctatcgctga taatggaatt ggtatagcca aagaggaaat caataaaata tttgaccaat
    10621 tctatcgctc taaatccaat gaacatcccg aaataaaagg aactggtcta ggattgtcca
    10681 ttgttagacg tttgtgcgac ttgctcgaaa taaatttaaa cattaatagc gaagaaaata
    10741 aaggaactac cgtatattta accctaaaaa aataagtatg acagatgcac atttacactt
    10801 agtagtcaat catttcccaa ttattgggtt atttttcgca attggaataa tggttttcgg
    10861 agtatttcta aaatcaaata ttttaaaaaa cactgcgtat gtaatgttaa tcataacagc
    10921 tatttttggc ttcctatcca tgtacactgg tgaaggagcg gaagaaatgg tggaagacat
    10981 gccaaacatt ggacacaaaa tcatccacga acacgaagaa cttgccgaaa agtttgtgct
    11041 ttttctctat gctgctgggt tattatctat tctgggtttg tatttcaact taaaaatgca
    11101 ttctaaagcc aatattgtag cttatgcaac acttgtggta gcaattattg ctggaatatt
    11161 tgcagtacaa gttggtactt ctggtggcga agtgcgtcat accgaaatta gaactgaaaa
    11221 agtagctcaa aacaacgata ttgaaaaaaa ctacagcgat gaacatacag atcatgacaa
    11281 taattaattt gtaaaagcat accttttaaa gcacatttct aatcaattgt tagagttttt
    11341 acaaccattt tgtcaaataa ataacgattc attaatttgt gattcattct ttgaaatatt
    11401 aatggtttaa aatgtagtta tactgtaaca ccaaacagaa attgtaagcg taacgaatgg
    11461 ttaaagcaat aaaaaatgca acggacgatt tgttagtaaa aaaaacaatc taatcccaac
    11521 tcaatctaac tctcactaca tacgaaaacg acgaaagaac attatagata tctggaattt
    11581 aacaactggg tgacacaaaa aaaacttcga aatggaacgt gacgtacaaa gctttcatca
    11641 tttcaagttt gttctggatt tatattatct cgaagattta ttatacaaat ttactcgtgg
    11701 agattggatt aaagtagaaa ttgacagata tggaaacttt actaacagcc ttgcaaccag
    11761 gtagttcaac tgagataaaa aaatatagat acatggcacc tcaattggcg ttcaagtcaa
    11821 agttacttcc aaaagtcatg caagaataag tcaacttctt ggtgataata cagagtgata
    11881 aattagagat tttgccacag tagcacaaca ataaaccgtc aaaaattaca ctaatagatt
    11941 cacatcttaa aaggttttca taacctgtta gatttaaaaa tatgaagtcg tacaaccaaa
    12001 aaaactttca caaatacacc ttttgccttt ggcaagaaat cccttttaca gaaatacaaa
    12061 gcctttttat caatcacaaa agtaaatctg gaagccaata catctttacc acaaaaggtg
    12121 tttaccgaat ttcaaaccac tggggaagag ctgcaaactg ccgttggaga ttacaaccac
    12181 tcgaaaatta caaaaatcaa caaataaaag tgggttttgc tcattggaca gacttctatc
    12241 ccaacaacga aatagaaaac ctatttttca tccaagtcaa tttcaataca aacgaagtaa
    12301 ctttcaatca caaagcagca ccagcctata acaaaacata cacacttcgc aacgctgctc
    12361 aaacatcaaa aataatcaaa ctaataaaac aaatactaac agaaaccact tgggctaaac
    12421 acttaaacta taataacatt gacactctaa gaagagaaat tatcaacaaa ctcctaacaa
    12481 ctaaatcctc tttttttgat attaaaaagc aatttacaat ctaaatagag caaaaaatag
    12541 ttacacaaat tattttgtac aaattattat ttgttacatt cgtatttgag taataacaat
    12601 gcgttattaa attatagctt ctatatatag tatatttgcg aattgaattt attttaaact
    12661 gtcaacggat ttaaaaccaa ataaaaagtg taaaaaaaca acaacaatat gataaataat
    12721 tttttaaagg taaatttgga aactaaacgc ttgatgaaca ctaatgttgt tgagaattat
    12781 tcgaaactga aattatcttt gtttgttttg ccactttttc ttttactttc tatcgtttta
    12841 ttccttatta gtgttgacgc tttatctgta aatggatatg ttgagattca aaaagaatgt
    12901 tttatttttc ttaactcaga attttcgcaa tttccaaatc ttatatacaa tctaacccaa
    12961 ataggtgatg cattaatttt tttatcattg ataagtgttt ttattgtcta tgcacctaaa
    13021 atatgggagt cattgataac tgcttcactt gtgtcggctg tgttttctcg cattctaaaa
    13081 gatatatttt ctataccaag acctgcagag gtttttgata atagtatttt tgttattgtt
    13141 ggaagaagac tacctggatt tagtagttta ccatcgggac attctattac tgtttttaca
    13201 ggacttacag tgttgttgtt tgcctttatg cccaaaaagt gggttgataa aattttatgg
    13261 tgcttcttta ttttagtttt aggatatagt attgctttta caagagtagg tgttggtgca
    13321 caccatccat tggatgttat ttctgggagt attattggtt atatctcagc acttttagga
    13381 atttttatta gtagaaaata taaaatatgg gcttggatta ataatataaa atcctatccg
    13441 ttttttattt tattattttc tatttgttgt tttgtgttaa ttcataaaat atacaaagaa
    13501 aatttggtca tattttattt ggcattaacc agtttaattg tttcactcta taaaattatt
    13561 tatgtttaca ttaaaaaata atttgaaaat tactcatttt gttttactaa tgagttgtct
    13621 taatttttta ttctttcatc tcccattttt cacttttgtt tttaagaatc tagaatataa
    13681 aagtttcagt ggtatttcaa taattattag tttaattatt ttaatgttgg tgctaaatgc
    13741 ttttgttttt tacctaattt tttccttatc tcgtcgtgtc ggaaagttt
//
LOCUS       DAOOBH010000055        13766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5742_length_13766_cov_8.662169, whole genome shotgun sequence.
ACCESSION   DAOOBH010000055 DAOOBH010000000
VERSION     DAOOBH010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13766
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5742_length_13766_cov_8.662169"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..2151)
                     /locus_tag="PK218_06930"
     CDS             complement(193..2151)
                     /locus_tag="PK218_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98274.1"
                     /translation="MKNSDLNKVVFYSKDDMAGGLELQKGEHVLRSDIKSNYTDINDI
                     FELYNIKKYIDNELYLKSWTNDDITNFKQKAAEFGKVIGQFMATINDSNVENLYENTL
                     QIYVHSFWELVNNQSVYKRISKSIFSNILSNEPYIIHEILTHKNIVDHYDKEIRLFLL
                     TYSQSAEILLSVYEVKDDFQKNQKFIPKSLTVDDKETILSNYLDSNDVNYNYIGLIQN
                     VRNRNDFKVSDKTRLKAKRLLNSETEKFYAENNGMKYGVSVSFSEKTSKVKDCVIDEN
                     FIINYTYSLDFIKQNCDPYSLFQNFKILFEYIDHQHRINLVSKKNKMGVFERIMGVHS
                     QNEYRVGTEFNLMEMTSHLQVVAYNKVLSDLGILLENVLYQVFTSSFQEKFGFASNAS
                     FSIPTAKSYFEKVRLLAPEFESVLKQFKLFVEDGSIDFELLQISSNPTAIKDIPSLNK
                     SKYLYFNEENKEMVDCINQFFSDQTLCALVEPFKEKHYHTFSDLLTKEKVKYSSYEAH
                     QKSQLNYLIDKGFINIDSNDFIQITNPARVIILKDLYLNEVASFYRYSFELQQEAQQM
                     EVEKIIFFDSTLFSKPEQDYFNYFLNKSEFTNGLDLRNSYLHGTQAKPDEIQKHKYAY
                     FTYLKLLILTFLKIEDDLIIYKAVKNRK"
     gene            complement(2446..3243)
                     /locus_tag="PK218_06935"
     CDS             complement(2446..3243)
                     /locus_tag="PK218_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98275.1"
                     /translation="MEELKRPDIIEVESFFNEYVEYFGGQVISKREDNLDDRPNADYL
                     FKSEKVIAELKCFQKDLFNNEEDISRIMNFLDRWEERKLIKQADKLKIILGSKKLPIE
                     CYYDLLSACTKTIDRALHKANKQIQESKRTFSLPDAKGLVLLCNDGNYFVQNDIFLGL
                     IANLMSRKYIESDIDGFVYFTINQVSTIPNSGLDWQLWVPSYRNENDSDLSQFVNDLG
                     HKFLNEFYTLKTGIESSEKFITDDTEVGYNAIKSMKYIPKEIIYKKK"
     gene            complement(3378..3794)
                     /locus_tag="PK218_06940"
     CDS             complement(3378..3794)
                     /locus_tag="PK218_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98276.1"
                     /translation="MSKRDERQKASDVFRESNFVFSKKVGFDVAFPQIEDLTVEVEES
                     RHGVGDWNRKSTYKKQNFPGEYINCSNSLCYNGGFSIGSILREMVRNKQTELETSKLC
                     QGNEGSPKGRRIYRKCMNFFKIKVSIKYKVESDRNG"
     gene            complement(4084..4767)
                     /locus_tag="PK218_06945"
     CDS             complement(4084..4767)
                     /locus_tag="PK218_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98277.1"
                     /translation="MKRIPIILLILFSATFSFSQSDSLEFDKKAKDRFVIWLIPSASK
                     NIYGIAIGPVGSEAICNRHYTKYSHGLNLQIPGQGFFQTFYINKMRFKDFYANENSDS
                     LSLQNTFPKRAIHNGLIISPLGTFTDQVNGISFSLWMSMGKKINGLSFNLLWNLYEQI
                     NGITIGFVNHTAATKGLQIGFVNKTIKLKGFQIGLWNKNEKRSLPLINWNFKDKKVQP
                     ITAPMPNSL"
     gene            complement(4925..6124)
                     /locus_tag="PK218_06950"
     CDS             complement(4925..6124)
                     /locus_tag="PK218_06950"
                     /inference="COORDINATES: protein motif:HMM:NF040149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98278.1"
                     /translation="MKCSKIANCCNTQNVGGNLKDSINEELYTMKKFIVTLLFTGILG
                     QIIFAQNNGDGVNLSSVDYFFKITKELNSGLEPSEDEWKTLFETSGYKISVTSDWSEN
                     VIRDMAIFAFVPGYQHHRDSILKISIVDNMDDYSKVFSKLTLINFMDMKKNLAELENF
                     RNSYDFNTLKEASKERLKSFLRNPVDSLVVFPSVNLLCQEADAQSKSKGIVMDFNLFF
                     KQVPTNENVDFLAHEMFHTYRRHFINEKFVKSNNLMKQIDILENEGIANMIDKTLNSQ
                     IQKLIILGYPQSIVDLYDSTYKNTPTKLQAMDSITCSFIRKEISEEEFDKRFKGFCIL
                     GGHPNSLYMTDIIKKAGLKDELITNFYRPVHFIKIYNRAANKEGTFVFSNEFVHYLEE
                     LELKYAD"
     gene            complement(6072..6452)
                     /locus_tag="PK218_06955"
     CDS             complement(6072..6452)
                     /locus_tag="PK218_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98279.1"
                     /translation="MKKSHPQKKYTLAGRFKPTHEPKLAREYFFLLPKKNFSFFSPTL
                     RCFNFFETAQPTTDKHSDGLMQTLTRDCNLTRTTEARTANSVLPQLAVICKIEAKCSY
                     QAFLLVDNEVLQNRQLLQHAKRWR"
     gene            complement(6452..12148)
                     /locus_tag="PK218_06960"
     CDS             complement(6452..12148)
                     /locus_tag="PK218_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HPW98280.1"
                     /translation="MKVKDIRLFRNWLKRQSRVQGLDENFIGFSNTGKETDDPKEIRG
                     FFNVFHAASPNIGDRLSTVLDMAQDSQAIYEFVQNAVDCDSTAFFMFYEDNHFIAINN
                     GRPFDLAGIRAILNFAQSTKTRDENIGKFGVGFKLIHRMVGAGNGLQELTQDYTGPIL
                     YSWSNKKQLEELLSANSVKEITADTNEKWDDSDTPWFFKILLTCVPILPNNLDTNLKD
                     ISYTDRTDLFTDEEFQTFKNFLNSIWEQNQDKFAEEDLNQGSLFYLQLGSDKEQKLDE
                     DFSYFKKGIQYSLSFVANLMSKKGLQKIYFKNEEPIVKDNIDVILESPFTIKTSSEEF
                     QEIIGSLKENDKTRDINIIFGYQKFENNAKYGDLRKSPNFYKFFPMGKEVCGLNFIVH
                     SNIFEIEASRREFVQKDKRNTFILEKLCDKLQDRLTEYQESKPETYNDIFLSILFSNE
                     PNHQQWDWITTTLYKPLIEFISKNCPTSKEGEYKPSSTVVIKNTKLALDPFDFGIVDK
                     FWFKWSKKDFDNIKTIDGNEDTSYYKVVKQTWDITNLIKEGNLNSIKAWYSKLDAKTK
                     NLFHSELLKKWKNETDVKFWNKIFEIPELIEQVIESNDFKVKSNYIKALTKLKLYSYK
                     IYSKDSYEFKLLKIAKEVLTTTTDIESFRTKVIIIDSQSIEHQLNDTRDNDKILFRES
                     NGKSWELNLSHVLPSYVNKSGLLSSIIKQFDELELSLHTFFGIGKQKSIDEIYRILSN
                     NYQNIVNEYQFAFLGLYSTEKNKDHFQYFDFEKLIVKDVMDFYFKNKFPFPKGYEKYI
                     ANDWIPELSIYPNDYALDSEKLPLKIRRWLEDDETEAKLKFLSEKVGVNIADSNVVKV
                     RKAIKRDGEVTQHHINLIYEKNTVLLLNTMKWIQREAITIKEDYQVSIVQKILKTFYI
                     KNDYSVDHPQLYISEVKNGDVTEYKFKNNNENDFYLDESKLNEIHKAGLTLKQIFEVA
                     QKNEKQIFDFRMYPNSELTKSDWTEIEIEISPDFQKIHNNSWSFDLSFYVEWEHSEDV
                     RILFHDGTMPQLTKFEDEILKEHNEGNFCKDESTGIIYININSAFDNIAKAEAIVESI
                     KELPDKYLSYHKKKSIDNAYWKWKEGENPNVTKIRKEQLKKAEEYSFQWLKALFDWEY
                     DATIGSHKPYTVWFEKVELNNNLIVLSECSFETIPARVEYHPEPIELYVKKKGQTRKI
                     VCSVANFNEFELTLQPIEQVDISYLQNFKTLQGYRANFKLPGDDVLMDRLRDNLFGAK
                     AIAPREGSIREYIQQNFSNNKISFLFGPPGTGKTTKVALDILSTLSFNSNSGMNTKIL
                     VLTPTNKAADVIIERIVELLSNEKKLEEVALTYYPEPSVKKLINYCKSIFSNREYQDI
                     LVRYGNSASSLLTEHNILKSKYTLKEILPNKVLATTVHRLAFDELAGNQLKDPSIGWT
                     HIVIDEASMVSLPHAVYTLLQFKTLPEVANKVGLLSTFTISGDPFQIQPVGKTPNYIE
                     HGIEGIKGWGTENIYTLFGLTNFSLARTPIGNFQIKKLYVQYRSVPSIGELFSKYKYD
                     GKIKHKKTKDIKEIELGDSYLDNINLISFPVFDEDTPTQEDIFNIQKYGEFSAYHIYS
                     IVLSCELASAIKLQNPSKTVSIITPYGTQARLTKEVSYAFRNQNNDNHFEVSTIHRYQ
                     GDENDVVILVMNPPKTNPYEFSHFNNSFLINVGISRAKEALIIFHPENITGYSEITEA
                     VKPLCENLNEIYCSEIEAKIFEGYKNNGTSKKIMDIVVVSGFQAFNVCDLKEFTNSGK
                     EYLFFADNRNLGKDEMRYANVIINLSKRNPLINYIKPRPGLLVEGIVTGIHKNNKTAF
                     IKIKNIKARAAIHCNSVSNTFVSDINQVIKVDQKIKAKIFSVDDKGITLTMRGVKQ"
     gene            complement(12615..13187)
                     /locus_tag="PK218_06965"
     CDS             complement(12615..13187)
                     /locus_tag="PK218_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98281.1"
                     /translation="MKNNEKRHIKINFIILVLLVIIGRLYDATTTYLYTPDLTNEANV
                     LVKLFDAGWTSIAIIQSTLIILILFLLYFYFFRFKPDLPREKNLNIKQFASFLFFNDT
                     ISFNKIFYKTPKNKKTLFAAIGYIVSMTLIAISFVVGTSTTFLIISDNYREIYKQGIP
                     YILYLLIVGLIVYFTIRFFKIEFKKYKQIT"
     gene            complement(13159..13629)
                     /locus_tag="PK218_06970"
     CDS             complement(13159..13629)
                     /locus_tag="PK218_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98282.1"
                     /translation="MKTKIIGTILPTILGILTVLGILVVFNLIVHNGDAFNSPDNGFF
                     KIFVPIATVIALTTQFTLTLHFWNKFKSKKKVWGLTLFQFTALLCIISGLIFGLVFWE
                     PNLGIKDLFLVSLTGIVAFSVYWTVNLFTLKQINKSQNLFKNTHRFEKQRKATY"
BASE COUNT         4085 a         2347 c         2228 g         5106 t
ORIGIN      
        1 ttttctgtct tcatgtagca aattgtccgc tatgtcgttt tttgcacttg ccgctaacgg
       61 ttgaggctat atgcagtaag ggattgcggg cttcgtctct atccgccgac ccaaacgcta
      121 ttgcggggta gaacgcttga attaaccact taaaccccaa tgttttatac acaatgttag
      181 cagttcgtcc tttcattttc tatttttaac cgctttataa attattaaat catcttctat
      241 ttttaaaaat gtcaagatta gcagttttaa gtatgtaaaa taagcatact tgtgcttttg
      301 tatttcatcc ggttttgctt gagttccgtg taaataacta ttccttaaat ctaagccatt
      361 tgtaaattca cttttgttca agaaataatt aaaatagtct tgttctggtt tagaaaacaa
      421 agtgctatca aagaatataa ttttctcaac ctccatctgt tgggcttctt gttgcaattc
      481 aaacgaataa cgatagaaag aagcaacttc attgagatat aaatctttaa gtattattac
      541 tcttgctgga tttgtgattt gaataaaatc gttgctgtca atatttataa accctttgtc
      601 tattaaataa tttaattgag atttttgatg tgcctcataa ctgctatatt ttactttttc
      661 ttttgtaagt aaatcggaaa aagtatgata atgcttttct ttaaagggtt caactaaagc
      721 acataaggtt tgatcagaga agaactgatt tatacaatcc accatttctt tattttcttc
      781 gttgaagtat aaatacttac tcttgtttaa gctaggaatg tcttttatag cggttggatt
      841 agatgaaatt tgtaatagct caaaatctat actcccgtct tcaacaaata gtttgaattg
      901 ttttaatact gactcaaact caggagctag taatcttact ttttcgaagt atgatttagc
      961 tgtgggtatt gagaaacttg cattgcttgc aaacccgaac ttttcttgga atgatgaagt
     1021 gaaaacttga tataaaacat tttccaataa aatacctaag tcgctaagta ctttattgta
     1081 tgctactact tgaagatgcg aggtcatctc cattagatta aattctgtac ctactctgta
     1141 ctcattttgg gaatggacgc ccataattct ttcaaatacg cccattttat ttttcttgct
     1201 tacaagattg attctatgtt gatggtctat gtattcgaat aaaattttaa aattttgaaa
     1261 tagcgaatat gggtcacagt tttgcttaat gaaatctaag ctatatgtat agtttattat
     1321 gaaattctca tcaataacac agtctttaac tttgcttgtt ttttcagaaa aactaactga
     1381 aacaccatat ttcattccat tgttttcagc gtagaatttc tcagtttcgc tattaagcaa
     1441 acgcttcgct tttaagcgtg ttttatcaga caccttaaaa tcattcctgt ttcttacgtt
     1501 ttggataaga ccaatgtaat tatagtttac atcattggaa tctaaatagt ttgaaagaat
     1561 cgtttcctta tcatcaacgg ttaaactttt aggaataaat ttttgatttt tttgaaaatc
     1621 atccttgacc tcataaaccg acagtaatat ttcagcagat tgagaatatg tcaataagaa
     1681 aagtctaatt tctttatcgt aatggtcaac tatgtttttg tgagttaaaa tctcgtgtat
     1741 tatgtatggc tcatttgaaa gaatgttgct aaaaatagac ttagaaattc gtttgtatac
     1801 actttgatta ttgactaatt cccaaaacga atgaacgtaa atttgcaaag tgttttcata
     1861 taaattttca acgttactat cgttaattgt agccataaat tgtcctatga cttttccaaa
     1921 ttctgctgct ttttgtttga aattggtaat gtcatcgtta gtccaacttt tcaaatacaa
     1981 ttcgttgtca atgtattttt ttatgttgta tagttcaaaa atatcgttta tatctgtgta
     2041 atttgatttt atgtcagacc gtaaaacatg ttctcctttt tgtagttcga gtccgcctgc
     2101 catatcgtct ttcgagtaaa atactacttt atttaagtca gaatttttca tttgtgctgt
     2161 gtctttttta aggtgactgc caacgtctta tttacgcaac aaatataaaa cattcgtaaa
     2221 tacaaaatat caaacttgcg taaaccacta atgtgtattt tgtatttggg ctgtttatta
     2281 tttcgttttc ctttttttgt ctttaaatag caaattgtcc gctatgtcgt ttttttttac
     2341 acttgcaccc aacgaccaag gctatgcgcg cctgcgattt gcgggagcaa aacctccaaa
     2401 ttgcacttta gccagcagat gagcataggc gcgtgttagt ggcagttatt ttttcttata
     2461 tattatttct ttcggtatat acttcattga tttaatcgca ttataaccaa cttcggtgtc
     2521 atcagttata aatttttctg aagattctat tcctgtcttc aatgtgtaaa attcgttcag
     2581 aaatttatga ccaaggtcat ttacaaattg gcttaagtcg ctatcattct cgtttctgta
     2641 agaaggcacc cataattgcc agtcaagacc actatttgga attgttgaaa cttgattaat
     2701 tgtaaaatac acaaaaccat cgatatcaga ttctatatac tttctactca ttaaatttgc
     2761 gataagccca agaaaaatgt cattttgaac aaaatagttt ccgtcattgc ataaaagaac
     2821 gagtccttta gcatcaggaa gcgagaatgt ccgttttgat tcttgaatct gtttatttgc
     2881 tttgtgtaag gctctatcaa tagtttttgt acaggcactt aataagtcat aataacattc
     2941 aatgggtagc ttcttagacc caagtataat ttttaatttg tctgcctgtt taattagttt
     3001 tctttcttcc catcggtcca agaagttcat tattctagaa atatcttctt cgttgttaaa
     3061 taaatctttc tggaaacatt ttagttcggc aattaccttt tcacttttaa ataaataatc
     3121 agcatttggt cggtcatcta aattatcttc ccttttagat attacttgtc caccgaaata
     3181 ttcaacatat tcgttgaaaa aactttcaac ttctataata tcaggtcttt taagctcttc
     3241 catttttatt tttttataat tgccactaac gttcccagca tatacgacgt cgcgaccgaa
     3301 gggagcaatg tctcgcgagg ggtaaggctc tgaaagagcc gaagcgtata tgttgtgtta
     3361 ggcgatgggg catgaaatta tccatttcta tcactttcta ccttgtattt gattgaaact
     3421 ttgattttaa aaaagttcat acacttccta taaattcgtc taccttttgg agatccttca
     3481 tttccttggc ataactttga tgtctctaac tctgtttgct tatttctgac catctcacgc
     3541 aatattgagc ctatcgaaaa tccaccattg tagcaaagtg aattgctaca atttatgtat
     3601 tcgccaggga agttttgttt tttgtaagta gactttcggt tccaatcgcc aacaccatgt
     3661 cttgattctt caacttcaac tgtcaaatcc tctatttgag gaaaagctac atcaaagccc
     3721 acttttttac taaatacaaa attgctttcc cggaatacat ccgaggcttt ttgtctttca
     3781 tccctttttg acattatttc atctcctttt gtttgccctt tcgcctaacg tttcatttac
     3841 gcaacaaata taaaacattc gtaaatacaa aatatcaaac ctgcgtaaac cactaatgtg
     3901 cagttttgta cttgggctgt ttattatttc gttttttttg tctttaaata gcaaattgtc
     3961 cgatgtgttg ttttttacac ctgccgctaa cggttgaggc tatatgcagt aagggattgc
     4021 gggatacaaa cctgtccacc gccacaaatt ttctgcgggg cactaccgtt tcaattttct
     4081 ctttcatagg ctattgggca taggtgctgt tataggctgc acttttttgt ctttaaaatt
     4141 ccaattaatc aacggtaatg accttttttc atttttgttc caaagtccga tttgaaatcc
     4201 ctttaatttt attgtcttat ttacaaatcc gatttgtaat ccttttgtcg ctgctgtatg
     4261 gttaacaaat ccaattgtta ttccatttat ttgttcgtat aaattccata gaagattaaa
     4321 tgaaagccca tttattttct tccccatgct catccacaat gaaaatgaga ttccattcac
     4381 ttgatctgta aatgtcccta atggtgaaat tataagccca ttatgaattg ctcttttagg
     4441 gaatgtgttt tgaaggctta aagaatcaga gttttcattt gcgtaaaaat ccttaaatct
     4501 cattttattg atataaaagg tttggaaaaa gccctgtcct ggtatttgta ggttcagccc
     4561 atgtgagtat ttagtataat gtctgttaca aattgcctct gatcctacag gtccaattgc
     4621 tatcccataa atattttttg atgctgacgg aattaaccaa atgacaaatc ggtcttttgc
     4681 ctttttatca aattctaaag agtcactttg cgaaaatgaa aaagtcgcag aaaacaaaat
     4741 cagtaatata atcggaattc ttttcattct ctcagtgttg cacataacgt ttggcggtat
     4801 agccagtaag ggattgcggg ggatttccca tcaaggtaca agaaaagtta aagcgggcta
     4861 caacccttcg catacctctg aaacccttat tggctatacc gcgtgttggc ggtagttttc
     4921 aatatcaatc agcatatttc aactccagtt cttctaaata atgaacaaac tcattgctaa
     4981 aaacaaaagt accttcttta tttgctgctc tattatatat tttaataaaa tgcaccggac
     5041 gatagaaatt tgttattagt tcatccttta accctgcctt tttaattata tcggtcatgt
     5101 ataaactatt gggatgtccg cccaaaatgc aaaacccctt aaatcgttta tcgaattctt
     5161 cttcgcttat ttctttgcga ataaaagagc aggttatgga atccatggct tgcaactttg
     5221 tcggtgtatt tttataagtt gaatcatata agtcaactat actttgagga tatcctaaaa
     5281 taatgagttt ttgaatctga ctgtttaagg ttttatctat catgttggca atcccttcat
     5341 tttctaatat atcaatctgc ttcatcaggt tatttgattt tacaaatttt tcgtttataa
     5401 agtgtctccg gtaggtatga aacatttcat gagctaaaaa atctacattc tcatttgttg
     5461 ggacttgttt aaaaaagaga ttaaagtcca tgactattcc cttggatttg ctttgtgcat
     5521 cagcttcctg acataataaa tttacagacg ggaaaaccac aagagaatcg accggatttc
     5581 ttaaaaatga cttaagcctt tcttttgagg cttcttttag agtgttgaag tcgtaactgt
     5641 ttctaaaatt ctctaattct gccaaattct ttttcatatc cataaaatta atcagcgtaa
     5701 gcttgctaaa tactttagaa tagtcatcca tgttgtccac tattgatatc ttcaaaatac
     5761 tatcacggtg gtgttgatat cctggcacga atgcaaaaat tgccatatcc cgaataacgt
     5821 tctccgacca atcagaagtt acacttattt tatatcctga ggtctcaaat aaggttttcc
     5881 attcatcttc cgacggttcc aaaccagaat tcagttcttt tgtaatttta aaaaaataat
     5941 cgacagaaga taggttaaca ccatccccat tgttttgagc aaaaatgatt tgacccaaaa
     6001 tgcctgtaaa aagtaatgta acaataaatt ttttcatcgt atataattct tcattaatgc
     6061 tgtcttttaa attacctcca acgttttgcg tgttgcagca gttggcgatt ttggagcact
     6121 tcattgtcaa ccagcaaaaa agcttgatag gagcacttag cttcaatttt gcatattacc
     6181 gccaattgcg gcaacacgct gttagcggtt cgtgcttctg tcgtccttgt caagttgcaa
     6241 tctctagtta aagtttgcat taaaccgtca gaatgcttgt ctgtcgtggg ttgtgcggtt
     6301 tcaaaaaaat taaaacatcg aagggtggga gaaaaaaatg aaaaattctt cttggggagg
     6361 agaaaaaaat actctcttgc aagctttggt tcgtgtgttg gcttgaagcg gcctgcaaga
     6421 gtatattttt tttgtgggtg agattttttc attattgttt tactcctctc atggttaaag
     6481 taattccttt atcatctaca ctaaaaattt tagcttttat cttttggtca actttaatta
     6541 cttgattgat gtcagaaaca aaagtgtttg aaaccgaatt gcagtgaatc gcggctctgg
     6601 cctttatgtt tttgattttt ataaatgcgg ttttattgtt tttatgaatt cctgtcacga
     6661 taccttccac aagcaaacca ggccttggtt taatgtaatt aattagcgga ttgcgctttg
     6721 aaagattgat aataacattg gcatatctca tttcatcttt tcctaagttc ctattatcag
     6781 caaagaataa gtattctttc ccagagttcg tgaattcttt caagtcgcaa acattgaaag
     6841 cttgaaagcc gcttaccaca acaatgtcca ttatcttttt tgaagtgcca ttgtttttat
     6901 atccttcaaa aatttttgct tcaatttctg aacaataaat ttcgttaaga ttttcacaca
     6961 agggttttac tgcttctgta atttcagaat aacctgtgat gttttcagga tgaaaaatga
     7021 taagtgcttc tttggcacga cttataccaa catttatcaa gaaggaattg ttgaaatgag
     7081 agaactcata agggtttgtc tttggtggat tcattacaag tatgacaaca tcattttcgt
     7141 caccttgata cctgtgaatt gtgctaactt caaaatggtt atcgttattc tgatttctga
     7201 atgcatagga gacttcctta gttaatcgtg cttgcgttcc gtatggtgta ataatgctga
     7261 ccgttttact tgggttttgc aattttattg ctgaagctaa ttcgcaagat aaaacaattg
     7321 agtaaatgtg gtatgctgaa aactctccgt atttctgtat gttaaaaata tcttcttgcg
     7381 taggtgtgtc ttcatcaaat actggaaaac taattaggtt aatgttatca agataagaat
     7441 ctccaagttc aatctctttg atgtcctttg tctttttgtg ttttattttt ccatcatact
     7501 tgtattttga aaaaagttca ccgatagagg gaacacttcg gtattgaaca tataattttt
     7561 tgatttgaaa atttccgata ggagttcttg ccaaagaaaa atttgtcagc ccgaaaagtg
     7621 tgtagatgtt ttcagttccc caacctttta taccttcaat accgtgttca atataattag
     7681 gtgtcttacc tactggttga atttggaaag gatcacctga aatagtgaaa gttgaaagca
     7741 atccaacttt attggcaact tcgggcaaag ttttaaactg caacaaagtg taaacagcat
     7801 gtggaaggga aaccattgac gcttcgtcaa ttacgatgtg tgtccatcca attgatgggt
     7861 ctttaagttg attgcctgca agctcatcaa aagccaaacg atgaacagta gttgccaaaa
     7921 ctttgttagg taagatctct ttaagagtat atttggattt taaaatatta tgttcagtca
     7981 ataatgaact tgcagaattt ccataacgaa ctaaaatatc ttgatattcg cgattactga
     8041 aaatgctttt gcaataattg attaacttct ttacagaggg ttctggataa taagttaatg
     8101 ctacttcttc caatttcttt tcgtttgaga gaagttcaac aattctttca ataataacat
     8161 cggcagcttt attggtcggg gtaagcacaa gtattttggt gttcattccg gagttactat
     8221 tgaaagaaag ggttgagaga atgtccagag caacttttgt tgtttttcct gttccggggg
     8281 gtccaaaaag aaatgaaatt ttattattac taaagttttg ttgaatatat tctctaatac
     8341 taccttctct tggcgcaatt gcctttgctc caaaaagatt atctctcaat ctatccatta
     8401 aaacatcatc acctggtaac ttgaaattag ctctataacc ttgaagcgtt ttaaagtttt
     8461 gcaaatagct gatgtcaact tgttctattg gttgtaatgt caattcaaac tcattgaagt
     8521 ttgcaacact gcaaacaatt tttcgtgttt gacctttttt cttaacgtat aattcaattg
     8581 gttctggatg gtattcaact ctcgctggaa ttgtttcaaa actacattcg cttaaaacaa
     8641 tgagattgtt gttcagttct actttctcga accaaacagt gtatggttta tgacttccaa
     8701 ttgtggcatc atactcccaa tcaaaaagcg ctttaagcca ttggaaagag tattcttcgg
     8761 ctttttttaa ttgttctttt ctaattttag tcacatttgg attttcccct tccttccatt
     8821 tccaatatgc attgtcaatg cttttctttt tatgatatga aagatattta tcgggtaact
     8881 ccttaatgct ctcaacaatt gcttctgctt tggcaatgtt gtcaaaagca gaattgatat
     8941 tgatataaat tattccagtt gattcatctt tgcaaaaatt accctcgtta tgttctttta
     9001 aaatttcatc ttcaaacttc gtgagttgtg gcattgttcc atcatgaaat agtatcctga
     9061 catcttcaga atgttcccat tcaacataaa acgacaaatc aaaagaccat gaattgttgt
     9121 gaattttttg gaagtcagga gaaatttcta tttcgatttc tgtccaatca gattttgtta
     9181 attcactatt tggatacatt ctaaaatcaa aaatttgttt ctcgttcttc tgagctactt
     9241 caaaaatttg cttcaatgtc aaacctgctt tatgaatctc atttaactta gattcatcaa
     9301 gataaaaatc attctcattg ttgtttttaa acttgtattc ggtaacatca ccgtttttta
     9361 cttcggaaat ataaagttgt gggtggtcta ctgaatagtc gtttttaatg taaaaagttt
     9421 taagaatttt ctgaactatt gaaacctgat aatcttcttt aattgttatc gcctcccttt
     9481 gaatccactt catagtgttc aaaagaagta cagtattttt ttcgtaaata agattaatgt
     9541 gatgttgagt tacttctcca tctcttttga ttgcttttct aactttgaca acgttgctgt
     9601 cagcaatgtt tactccgacc ttttcagaca agaatttcaa ttttgcttct gtttcatcat
     9661 cttctaacca cctacgtatt tttaacggaa gtttttcaga atccaaagcg taatcatttg
     9721 gataaattga taattctgga atccaatcat tagcaatata tttttcatat cctttgggaa
     9781 atggaaattt atttttgaaa taaaagtcca ttacatcttt tactattaac ttttcgaagt
     9841 caaaatattg aaaatggtct ttgttttttt cagttgagta aagtcctaaa aaagcaaact
     9901 gatattcgtt gactatattc tgataattgt tgcttagaat cctgtaaatt tcatcaatag
     9961 atttttgttt gccgattcca aaaaatgtat gtaatgacaa ttcaagttca tcaaattgtt
    10021 ttatgattga gcttagcaat ccacttttat ttacgtagct aggcaaaaca tgcgatagat
    10081 taagttccca tgacttgcca ttcgattcac gaaaaagtat tttgtcgtta tctcttgtat
    10141 cattcagttg gtgttcaatg gattggctgt caataataat tacttttgtt ctgaatgatt
    10201 caatatcggt tgtagttgtt aaaacttctt tagcaatttt aagtagctta aattcatagc
    10261 tatctttgga atatattttg tatgaataaa gtttgagttt tgtaagtgct ttgatataat
    10321 tggactttac tttgaagtcg tttgattcaa taacttgttc aatcaactca gggatttcaa
    10381 atattttgtt ccaaaatttt acatcagttt cgtttttcca ttttttaagc agttcactat
    10441 gaaataaatt tttggtttta gcatccaatt ttgaatacca cgccttgatt gaattcagat
    10501 tgccttcttt aatcaggttt gtaatgtccc aagtttgctt gactacctta taataactgg
    10561 tgtcttcgtt gccgtcaatt gttttaatgt tatcgaaatc ttttttgctc cacttgaacc
    10621 aaaacttatc tacaattcca aaatcaaaag ggtcaagtgc cagtttggta tttttaatta
    10681 ctactgttga cgatggctta tattcacctt ccttgctagt aggacaattt ttggaaatga
    10741 attcaataag gggtttgtaa agtgtagttg ttatccaatc ccattgctga tggttgggtt
    10801 cattagaaaa caagattgat aaaaaaatgt cattgtaagt ttcaggtttt gattcctgat
    10861 attcagtaag tcggtcttgt agtttgtcgc aaagcttttc aaggatgaaa gtgttccgct
    10921 tgtccttttg aacaaattcc cttctgcttg cttctatttc aaaaatgttt gaatgaacta
    10981 taaaatttag cccgcaaact tcttttccca ttggaaaaaa tttatagaag ttgggagatt
    11041 tcctcagatc gccatattta gcattatttt caaatttttg atatccaaaa ataatattaa
    11101 tgtctctggt tttatcattc tcttttaatg aaccaatgat ttcttgaaat tcctcagaag
    11161 atgtttttat cgtaaatgga ctttctaaaa taacatcaat gttgtcttta acaattggtt
    11221 cctcattctt gaagtaaatt ttttgcaagc ccttttttga cattaggttt gcaacaaaac
    11281 tcaaagaata ttgtattcct tttttgaagt aagagaaatc ctcatcaagt ttttgttcct
    11341 tatcagatcc aagttgcagg tagaataagc tgccttgatt taaatcttct tctgcaaact
    11401 tgtcttggtt ttgctcccaa atgctgttta aaaagttctt aaaggtctga aattcttcgt
    11461 cagtaaacag gtcggttcta tcggtgtaag aaatgtcttt aagattggtg tcaagattat
    11521 tgggaagaat gggaacacaa gtcaaaagaa ttttaaagaa ccaaggtgta tcagaatcat
    11581 cccatttttc atttgtgtcc gctgtaattt ctttgactga atttgctgaa agcaattctt
    11641 caagttgttt tttatttgac caactataaa gaataggacc agtgtaatct tgtgtaagtt
    11701 cttgcaatcc gtttcccgca ccaaccatcc tgtgaattag tttgaatcca actccaaact
    11761 ttccaatgtt ttcatctctt gttttggttg attgagcaaa atttaatatg gctcttattc
    11821 ctgcaaggtc aaacggtctc ccattgttaa tagcaataaa gtgattgtct tcataaaaca
    11881 taaagaatgc agttgaatcg cagtcaacag cgttttgcac aaactcataa atggcttggc
    11941 tgtcttgtgc catatcaaga acagtagaaa ggcggtcacc aatatttggt gatgctgcat
    12001 gaaaaacatt gaagaaccca cgaatttctt ttgggtcatc tgtttccttt ccagtgtttg
    12061 aaaatcctat aaaattttca tccagccctt gaactctaga ctggcgtttg agccaatttc
    12121 ggaatagtcg tatatctttt actttcatga cttaacgaag ataaataaag cgttggtaga
    12181 ttgcactctt atgactgcca attgctttgg tttgtgggaa gggtggcagg caaatgtgtg
    12241 taatgtgcgt aggcatagaa tttttaattt ttttgtggag tggggtgttt taaatttttt
    12301 attgggtagg catgagtttc ggactgtccg cccgaactgt cccgaattta tgttgcctat
    12361 atgtcgttgt ccacctgtca aaatttattg tcttaaagtt tattggcagc ccgtctgttt
    12421 aatggttcca ccaccgtccc aatagcatga ccggtaacgt tttggcggta tggttagttg
    12481 ccgatttcgg agtacgtcac tgtcaagcca cgatgaagtt tgaagcgaac gacgaacttt
    12541 caatttacca agctctcggc aataaaatat accgccggtt atggtgcgtt gtttcttgtt
    12601 aatcaagtac attatcaagt tatttgttta tattttttaa attcaatttt gaaaaatctt
    12661 attgtgaaat aaacaattag tccaacgatc agcaaatata aaatataagg aatgccttgt
    12721 ttatatattt ctctgtagtt gtcgcttatt attaaaaaag tcgtagaagt cccaacaaca
    12781 aaacttattg ctattagagt cattgaaacg atataaccaa ttgctgcaaa aagagttttt
    12841 ttattttttg gtgttttgta gaaaatttta ttaaacgaaa tggtgtcgtt aaagaataaa
    12901 aatgaagcaa attgtttgat attcaaattt ttctcacgtg gtaagtctgg tttaaatcta
    12961 aaaaaataaa agtacaagag aaaaagtatc aatataatga gtgtgctttg aataatagca
    13021 atacttgtcc atcctgcatc aaatagtttc actaatacat tcgcttcatt agtcaagtca
    13081 ggagtatata aataagttgt tgttgcatca taaagtctgc caataattac aagcaaaaca
    13141 agaattataa aatttatttt aatatgtcgc ttttcgttgt ttttcaaaac gatgtgtgtt
    13201 tttaaataag ttttgacttt tatttatttg ttttaaagta aacaaattta ctgtccaata
    13261 tactgaaaaa gcaacaattc cagtcagaga aactaaaaat aaatctttaa ttcctaagtt
    13321 tggttcccaa aacactaatc cgaaaatcag tcctgatatt atacatagta acgcggtgaa
    13381 ttgaaaaagt gtcaaacccc aaactttttt cttagattta aatttattcc aaaaatgaag
    13441 agtcaaagta aactgagttg tcagtgcaat aactgttgca attggcacaa atattttaaa
    13501 aaaaccattg tcaggagagt taaaagcatc tccattgtgt acaataagat taaatactac
    13561 caaaattcca agcactgtca atattccgag tattgtcggt aaaattgtcc caatgatttt
    13621 tgttttcata cttcttattt tttgttttct ctttaaacgg tcaaactcat taaaatattt
    13681 tgtctgtctt ttttacaatg caccataacg cttcgcggct tggcgtagtg gcggattttt
    13741 agcacaaaag ttcaatcgaa gaactg
//
LOCUS       DAOOBH010000056        13727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5765_length_13727_cov_6.669617, whole genome shotgun sequence.
ACCESSION   DAOOBH010000056 DAOOBH010000000
VERSION     DAOOBH010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13727
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5765_length_13727_cov_6.669617"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /gene="clpX"
                     /locus_tag="PK218_06975"
     CDS             complement(<1..923)
                     /gene="clpX"
                     /locus_tag="PK218_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPW98283.1"
                     /translation="MAREVLECSFCGRKKPETNLLIAGINAHICDRCIEQANGIVVEE
                     LKTTTKVKVSSDLILRKPKEIKSFLDQYVIGQDQTKKVMSVAVYNHYKRLVQVQNSEE
                     VEIEKSNIIMVGQTGTGKTLVAKTIAKMLDVPLAIVDATVLTEAGYVGEDVESILTRL
                     LQAADYDVTKAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQALLKLLEGTVVNVP
                     PKGGRKHPDQKFVEVNTKDILFIAGGAFDGIERIISKRLNRQAVGYSTSKNVDNIDKD
                     NLLQYIIPKDIKDFGLIPEIIGRLPVLTHMDP"
     gene            complement(951..1610)
                     /gene="clpP"
                     /locus_tag="PK218_06980"
     CDS             complement(951..1610)
                     /gene="clpP"
                     /locus_tag="PK218_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPW98284.1"
                     /translation="MNYGKEFKKFAIKKHGVNSLYFDKIITNMTPAVIEERQLNVTQM
                     DVFSRLMMDRIIFLGTGVDDYVANVIQAQLLFLESTDASKDIQIYINSPGGSVYAGLG
                     IYDTMQYIKPDVATICTGMAASMGAVLLCAGAEGKRSALPHSRVMIHQPSGGAQGVAT
                     DMEINLKEMLKLKDELYQIISKHSGQTFDKVHKDSERDYWMIADEAKEYGMIDEVLRR
                     S"
     gene            complement(1708..3036)
                     /gene="tig"
                     /locus_tag="PK218_06985"
     CDS             complement(1708..3036)
                     /gene="tig"
                     /locus_tag="PK218_06985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPW98285.1"
                     /translation="MNITRNNVDALNAVVTVEVSKADYSDKVQKVLTDYRKNAAIPGF
                     RKGTVPMSLIQKQYGKAVLLEEVNKVLQSNLNNYLAQEKLDILGNPLPKVTEDFNWDV
                     EDYKFEFELGLAPEFSVNLDAKNEVVSYKIIADDKMLNEQITRIQKQYGKLIAKDTVE
                     DGNDVSGIFANEEKGINNRTTLSLDAFADKNTAKLFIGKKIGDVITLKTKGLFDDDHK
                     LMDYLAVGHDDVHGLDVDVTFTIDEVTTHEPAVLDQELFDKLFGPKIVTSVDELKAKI
                     KEDAEKQFVQQADQKFLNDVVESLIENTKFDLPSQFLKKWIQVAGENPLTVEEAEAEY
                     AKSEKGLRYQLIEGKIMAENNLQITFEDLKSYTSEMIKKQMAQFGQMNPSDEDVQGIV
                     ARVMSNQDEVKRLSEQVMSEKMLNLYKEKVATKSKEVTFDEFVKALYGEI"
     gene            complement(3119..3463)
                     /locus_tag="PK218_06990"
     CDS             complement(3119..3463)
                     /locus_tag="PK218_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPW98286.1"
                     /translation="MKLFFRILITAILVMVISYLMIGINVDEFTTALSVAIVLALLNF
                     FVKPILVLFTLPITIFTLGLFLLVINASIILLCDHFINGFEVSSFWNAMLFSIILSLS
                     QSLVFKLTGEAK"
     gene            3543..4358
                     /locus_tag="PK218_06995"
     CDS             3543..4358
                     /locus_tag="PK218_06995"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98287.1"
                     /translation="MKKFLLSIILVSSFSGFSQILSEDFESGTFPPDGWTTSTLVPTR
                     PWGLTTDIFNATGQATFDINGIGTAAIGWIAQDNVADLTSPVFSLVGYGDAIFDFNIK
                     IGFEYMVDPFPNGDLEVQISTDGSNWTTLWVEEDQGVFVDYETLSISLDLAAYLGEAN
                     VQVRFLYSGNDADSLSVDDIMITGNLGVQQVLASSFTTFPNPTRDVISISNNENIVLN
                     SIKITDINGRTVKQIQADEINAAPINVSELSSGVYFMTISSNAGNAVKKFIKN"
     gene            complement(4666..6294)
                     /gene="pruA"
                     /locus_tag="PK218_07000"
     CDS             complement(4666..6294)
                     /gene="pruA"
                     /locus_tag="PK218_07000"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPW98288.1"
                     /translation="MPKGFFNVPKAVNEPVKSYAPNSPEKTAVLAAYRKMWNETIEVP
                     MYIGKEEVRTGNTNNMSAPHDHQHIVGTYHLAEKKHIDQAIVEALEARKKWSQMPWEQ
                     RAAIFLKAAELIAGPYRARINAATMIAQSKTIFQAEIDASCELIDFLRFNVEFMTQIY
                     QDQPKSTSDVWNRVEYRPLEGFVYAITPFNFTAIAANLPASAALMGNTVVWKPSDSQV
                     FSAKIIIDVFREAGVPDGVINVVFGDPAMISDTIFNHPDFAGVHYTGSTFVFKEIFKK
                     IGNNIHTYKTYPRIVGETGGKDFIIAHPSANVKQVVANSIRGAFEFQGQKCSAASRAY
                     FPKSLWNAIKEEMGKELASIKMGSPEDFGNFVTAVIHEGSFNKLASFIDQAKKDADAE
                     IIFGGNYDKSKGYFIEPTVIVTTNPKYATMETELFGPVLTIYVYEDAKWSETLKLIDE
                     TSEYALTGAVFSQDRYAIVEAANALQNSAGNFYINDKPTGAVVGMQPFGGARASGTND
                     KAGSAQNLLRWVSPRTIKETFVTPTDYRYPFLGE"
     gene            complement(6418..6804)
                     /gene="apaG"
                     /locus_tag="PK218_07005"
     CDS             complement(6418..6804)
                     /gene="apaG"
                     /locus_tag="PK218_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HPW98289.1"
                     /translation="MVTQITRGIKISVTTSFEGTYFKNHKIHFAFSYEITIENHSKDS
                     VQLLSRHWEIFDSLNDTETVDGEGVIGKKPVLKPGELHTYSSGCLLSSPFGAMRGHFN
                     MINFTSTRNFRVIVPTFKLSAAFALN"
     gene            complement(6877..8130)
                     /locus_tag="PK218_07010"
     CDS             complement(6877..8130)
                     /locus_tag="PK218_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HPW98290.1"
                     /translation="MSKKILFSSLFLFVFLTAFSQEKEIAPPYNIKTVTFVQNAQNTI
                     PVFSLGDSFELQFDDLFGNEANYYYTLTHCNYDWKPSQLAKAEYLNGFDDQRIQNYTN
                     SLNTLQLYSHYKLTLPNKFTQLRVSGNFVLKILDDDKNVVFTKKFIVYENLVSVPLEV
                     KRARDVSMLNYKQNLEFSIKSSNINFQSPLQNVKVLLLQNGRFDNAIKNIKPMFTIGN
                     DLIYKYDKETQFWAGNEFMFFENKDIRAANNNIARVDSNQGGVYNSYLYTNEARANKP
                     YTYFPDFNGNFIVNNIGAEQNEIEADYAWVFFSLSAPNYFGKKSIYVNGLFNNYATND
                     ENKMVYNEKKGVYEKAIMIKQGFTNYQYLISDGNGNLDEENAIDGNFAVTENNYLVLV
                     YYKENNQRYDRVIGRGSASSLDITY"
     gene            complement(8195..8965)
                     /locus_tag="PK218_07015"
     CDS             complement(8195..8965)
                     /locus_tag="PK218_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPW98291.1"
                     /translation="MKSVFKFILNTIPRPILIRMSIVVRPILAFLLKGNRFTDPIDGK
                     SFRMFLPYGYGNQRNNVLSPSTLSLERHRLLWLYLQNETDFFTTTEKKKVLHFAPEQE
                     FYKRFKKQTNIDYTTTDLLSPLADVKADICNLPFEDNTYDIIFCNHVLEHIPDDTKAM
                     QELYRVLKPNGMGIFQIPQDLSRATTFSDDAIIDQKERAKIFGQYDHVRVYGRDYFDK
                     LRSIGFKVIEEDYTSKIAPELVEKYCLANGEIIPVCFK"
     gene            complement(8984..9358)
                     /locus_tag="PK218_07020"
     CDS             complement(8984..9358)
                     /locus_tag="PK218_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPW98292.1"
                     /translation="MENFYLKEETFKIIGICMEVHKILGKGHSEVVYKDALEYEFKIN
                     SIPYEREKIYKIQYKDIILPRKYIADFVVYDEIIFEVKAISELTNSEIKQTLNYLASS
                     KNKLGLLLNFGEDSLKYKRVIL"
     gene            complement(9370..10188)
                     /gene="map"
                     /locus_tag="PK218_07025"
     CDS             complement(9370..10188)
                     /gene="map"
                     /locus_tag="PK218_07025"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPW98293.1"
                     /translation="MIIPKTAEEIELMRQSALLVSKTLGMIAAEIKEGVTTLALDKFA
                     EEFIRDHKALPGFKGLYGCPSTLLTSVNEQVVHGLPTNRPIKNGDVVSVDCGVLMNDF
                     YGDHAYTFEIGDVNPETKKLLQVTKESLYIGIREFKAGNRVEDVGSAIQKYTESFGYG
                     VVRELVGHGLGRKMHEAPEMPNYGKRGKGKLFVEGMVVAIEPMINMGTKNIKTLSDKW
                     TIVTRDGKPSAHFEHNVALINGKPELLSTFQYIYAVLGIQSDEENEFRNTNSTN"
     gene            complement(10231..11616)
                     /locus_tag="PK218_07030"
     CDS             complement(10231..11616)
                     /locus_tag="PK218_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW98294.1"
                     /translation="MKLLLSIFLLLGFNLFGQNKASNEFTFNEYLGYVKKYHPLVKLA
                     DLNISEAQASLMMARGGFDPKIEIDYEQKRFKDSEYYSILNSSFKIPTWYGIELKAGF
                     DNNDGVYLNPENTVPNQGLTSVGIAVSVGQGLFINQRMADVRKGKIQLKLGQAERNLE
                     ATTVLYNASLAYFNWKKNYDEVKLYENYATYAATRFKVIKISIEQGDKPAIDSIEAGI
                     IVRNRALSLEDSKLKLAKATLELSNFLWLDNNIPLELQDDLIPENKLESTISETLRTN
                     DLLQNASLENHPKINAWQTKIDMLTIEQKLKSNLLLPKIDLGYSYLSEPSYFEKYRFE
                     DYKVGVNFSFPLFLRKERGSLKLAKYKVQDAEYSLKVVRLQLKNKLKAQQTEINSLIK
                     QINIIENLVNDYQTMLRSEERLFSFGESSIFLINARENNLVSSQLSKINVANRFFISN
                     AELFKIMANPD"
     gene            complement(11616..12956)
                     /locus_tag="PK218_07035"
     CDS             complement(11616..12956)
                     /locus_tag="PK218_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPW98295.1"
                     /translation="MLNLSENNSIKDTTDKYKTVKDLRNRPHYKILNRIIIWFTLLGV
                     LILFLPWTQNISGKGAVTTLKPNQRPQTVQTAIAGRIEKWYVKEGDFVKKGDTILFIS
                     EIKEDYLDPNLVQNTESQLQAKEMATESYGSKVKSLASQIEAIRNEKKLKLEQAKNKV
                     RQAILKIKSDSIDLVAVRTQIKIANTQFNRSLTLNKEGLKPMTDVEEKRLKLQETEAK
                     IITQENKLITAENELINAKVEINRIIAEYNEKESKASSDKYTALSSQYDTKAQVNKLQ
                     NQVQNYQIRNGMYYIKAPQSGFVNRALQSGIGETIKEGTQIVSIMPSDYEIAVETFVN
                     PNDLPLINKGEKVRVWFDGWPTIVFSGWPNMSYGTFGGKIVAIENFISENGKFRVLIA
                     PDETEAPWPKQISIGSGAQTLALLDNVPVWFEIWRTLNGFPPNYYNPKSDKAKK"
     gene            complement(12959..>13727)
                     /locus_tag="PK218_07040"
     CDS             complement(12959..>13727)
                     /locus_tag="PK218_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW98296.1"
                     /translation="LYDVLTAVEKIGQITDLKIENTTDVPNEICYSNITLEVEKLCYK
                     FQDSNNFVLNTINFKLEPSEKIIIKGNNGSGKTTLIRILSGLLQPTSGTFFINDDTYR
                     KINLDQYRSQISVIISGETPFEGTILENITFNNPNISQEDLKWAIDSVQLGEYIKSLP
                     KGLDTKIYPEGKQLSSSNAQKILLARSIITKPKILFLEEPFDKMDDITANELIDFITD
                     SNQKWALVITSKNPYWEKKCTRIIIMQEGEIYNDLKK"
BASE COUNT         4226 a         2298 c         2084 g         5119 t
ORIGIN      
        1 ggatccatgt gtgtcaaaac tggtaaacga ccaataatct ccggaatcaa accgaaatct
       61 ttaatatctt ttggaatgat gtattgtaac aaattgtcct tatcaatatt atcaacattt
      121 tttgaagtac tataacccac cgcctgacgg ttcaaccttt tggatataat tctttcaatt
      181 ccgtcaaaag ccccaccagc aataaataaa atatctttag tgttgacttc tacgaatttt
      241 tgatcaggat gttttcttcc accttttggc ggaacattga ccactgtacc ttccagtaat
      301 ttcaacaaag cttgttgcac tccttctcca gacacatcac gtgttatgga tggattatca
      361 cttttacgag caattttatc aatttcatct ataaaaacaa ttcctctctc ggctttggtt
      421 acatcatagt ctgcggcttg aagcaagcga gttaaaatac tctctacatc ttcccctaca
      481 taacctgcct cagtaagcac tgttgcatca acaatagcca aaggcacatc taacattttt
      541 gcaatggttt ttgcgactaa tgtttttcct gttccagttt gtccaaccat gataatattg
      601 cttttttcaa tttcaacttc ttctgaattt tgaacttgaa ctaagcgttt ataatgattg
      661 taaacagcaa ccgacattac ttttttcgtt tgatcttgac caataacata ttgatccaaa
      721 aaagatttaa tttcttttgg tttgcgtaat attaaatctg aagaaacttt aactttggtt
      781 gttgttttta attcttcaac aacaatacca tttgcttgtt caatacatct atcacagata
      841 tgtgcattaa ttcctgcaat aagtaggttt gtttcgggtt tctttcttcc gcaaaatgag
      901 cattctaata cttctcttgc cattttatat aaagtttatt tgggtgcctt ttaacttctt
      961 ctaagaactt cgtcaatcat tccgtattct tttgcttcat cggcaatcat ccaataatcg
     1021 cgttccgaat ctttatgcac tttgtcaaaa gtttgcccag aatgttttga aataatttga
     1081 tacaattcat ctttcaattt caacatttct ttcaggttga tttccatatc tgttgcaaca
     1141 ccttgtgcgc ctcctgaagg ttggtgaatc ataactctag aatgtggtag ggcagaacgt
     1201 tttccttctg cacctgcaca cagcaatacg gctcccattg aagctgccat cccagtacaa
     1261 attgttgcta catccggttt gatgtattgc atagtatcat aaattccaag tcctgcataa
     1321 acacttccac ctggagaatt gatatatatt tgaatgtctt ttgaagcatc tgtgctttct
     1381 aaaaacaaca attgagcttg aattacattg gcaacataat catcaacacc tgttcctaaa
     1441 aaaataattc tatccatcat taatcttgaa aaaacatcca tttgagtaac atttaattgg
     1501 cgttcttcaa taactgctgg tgtcatgttt gtgatgattt tatcaaaata caaactgttt
     1561 acaccgtgtt tttttatagc aaatttttta aattcttttc cgtagttcat cgtttttaat
     1621 aaattataaa tgaatgaatt aatattttat ccatacaaaa aacgtcaaat cttgacagat
     1681 tcgacgttca aagataataa tttttattta aatctcacca tacagagctt taacgaactc
     1741 gtcgaaggta acttcttttg attttgtcgc tactttttct ttgtataagt tcaacatttt
     1801 ttcgctcata acttgttcgg aaagtctttt tacttcgtct tgatttgaca ttactctagc
     1861 tacaatgcct tgaacatctt catcagatgg attcatctgc ccaaattgtg ccatttgttt
     1921 tttaatcatt tctgaggtgt aagactttaa atcttcaaaa gtaatttgca aattattttc
     1981 tgccataatt ttaccttcaa ttagctgata acgaagtcct ttttcggatt ttgcatattc
     2041 agcttcggct tcttcaacag ttaacggatt ttcgccagcg acttgaatcc attttttaag
     2101 aaattgagaa ggcaaatcaa attttgtgtt ttcaattaaa gattcaacta catcatttaa
     2161 gaatttttgg tcagcttgtt gcacaaattg tttttcggca tcttctttaa tttttgcttt
     2221 caattcatcc actgatgtga ctatttttgg tccaaataat ttgtcaaata attcttggtc
     2281 taaaacagcg ggttcatgag ttgttacttc atcaatggta aaggtaacat caacatccaa
     2341 tccatgaaca tcatcatgac caaccgctaa ataatccatt agtttatggt cgtcatcaaa
     2401 caagccttta gtctttaatg tgataacatc gcctattttt ttaccaataa ataatttggc
     2461 agtattttta tccgcaaaag catctaagga taaggttgtt ctgttattaa ttcctttttc
     2521 ttcatttgcg aaaattcctg agacatcatt gccgtcttct acggtgtctt tagcgattag
     2581 cttgccgtat tgtttttgaa ttcttgtaat ttgttcgttc aacattttat catcagcaat
     2641 gattttatag ctaacaacct cattttttgc atctaaatta actgaaaatt ctggagctaa
     2701 acccaactca aactcaaatt tataatcttc aacatcccag ttgaaatctt cggttacttt
     2761 tggtaatgga tttccaagga tgtctaactt ttcttgcgct aaataattat taagattgct
     2821 ttgaagcact ttgtttactt cttccaacaa aacggctttc ccatattgtt tttgaattaa
     2881 actcattggt acagttcctt ttctaaagcc aggaatagcg gcgtttttac gatagtcggt
     2941 caatactttt tgtactttat ctgaataatc agctttggat acttcaacag ttacaacggc
     3001 atttaaggcg tcaacgttat ttcttgtaat gttcattttc tatatactaa aattgggtgg
     3061 caaaattaac acatttctgc aactcaacca agtttttatt ttattgaatt ttaaagtatt
     3121 attttgcttc accagtaagc ttaaaaacta aagattggga taaagataaa atgatactga
     3181 ataacatggc attccaaaaa gaagatactt caaatccatt aataaaatga tcgcataaca
     3241 aaattatgga tgcatttatc actaataaaa acaaccctaa agtaaaaatt gttataggta
     3301 atgtgaataa aacaagaatg ggttttacaa aaaaattaag taaagctaaa acaatggcaa
     3361 ccgataaagc tgttgtaaat tcatcaacat ttatgcctat cattagatag gaaataacca
     3421 taactaaaat ggctgtaatt aaaattctaa agaataattt catgaatgga tattgctaaa
     3481 tttcaataaa attaaaaaaa attgtgtata tttgataaaa ttaactttta aaaaacaact
     3541 ttatgaaaaa atttttactt tcaattattt tagtttcttc attttcaggc ttctctcaaa
     3601 ttttatcaga agactttgaa tctggtacat ttccacctga tggttggaca acttcaactt
     3661 tagtgcctac tagaccttgg ggattaacaa ctgatatttt caacgctaca ggtcaagcaa
     3721 catttgatat taatggaatc ggcacagctg caattggatg gattgctcaa gataacgttg
     3781 ctgatttaac ttcgcctgtt tttagtctag ttggatatgg tgatgctatt tttgatttta
     3841 atattaaaat cggatttgaa tatatggttg atccttttcc aaatggtgat ttagaggtac
     3901 aaattagcac agatgggtca aattggacta ctttatgggt tgaagaagat caaggtgttt
     3961 tcgttgatta cgaaactcta tcaatttcat tagacttggc tgcttatctt ggagaagcaa
     4021 atgtacaagt cagattctta tatagtggta atgatgcaga ctcactttcg gttgatgata
     4081 ttatgattac tggtaattta ggagttcaac aagttttagc ctctagcttt acgacttttc
     4141 caaaccctac acgtgatgta atttcaattt ccaacaacga aaacattgtg ttaaattcta
     4201 tcaaaataac tgatattaat ggaagaacag ttaaacaaat tcaagctgac gaaattaatg
     4261 ctgcacctat taatgtttca gaattaagct ctggagttta cttcatgaca atttcttcaa
     4321 atgctggaaa tgcagtgaaa aaattcatta aaaattaaac tgaaaaagat aatattatag
     4381 tctttttaga atacattgcc ccaaaaagtt aaaaactctt tggggctttt tttatgaatt
     4441 ttaaaaatta ttttgaatat aaattaaaat ctgacaaacg tttattcaca aaataaaaaa
     4501 ataaaccaag ttgttattag cagaattgta tttaggtggt aattataaaa ttcaacatat
     4561 tataaacaaa aagacaagtt gcatttgaat tccaatcaaa agaattaaat gaaaaaatga
     4621 gtccgctaaa atggcgaact cattaactat taaaaatact ttatactatt ctcccaaaaa
     4681 cggataacga taatctgttg gcgttacaaa cgtttctttg atagttcttg gtgaaaccca
     4741 acgcaataag ttttgtgccg aacctgcttt gtcattggtt cccgatgctc ttgcgccacc
     4801 aaaaggttgc attccaacaa cagcacctgt tggtttgtca ttgatgtaga aattaccagc
     4861 cgagttttgt aatgcatttg ctgcttcaac aatagcatat ctatcttgac taaagactgc
     4921 gccagttaag gcatattccg aagtttcatc tattaatttt aaggtttccg accattttgc
     4981 atcttcataa acatatatgg ttaatactgg gccgaaaagt tcggtttcca tagttgcata
     5041 ctttggattg gtagtaacaa taacggttgg ttcgatgaaa tatccctttg atttatcata
     5101 atttccacca aaaataattt cggcatctgc atcttttttg gcttgatcaa taaaacttgc
     5161 tagtttatta aacgaacctt catgaattac cgctgtaaca aaattaccaa aatcttcggg
     5221 cgaacccatt ttgatgcttg ctaactcttt gcccatttct tctttaatag cgttccaaag
     5281 tgattttgga aaatacgctc ttgaagcggc agaacatttt tgaccttgaa attcaaaagc
     5341 accacggata ctgttggcga ctacttgttt tacatttgcc gatggatgag cgatgatgaa
     5401 atcttttcca cctgtttccc ccacaattct tgggtaggtt ttgtatgtat gaatattgtt
     5461 tccgattttc ttgaaaattt ctttgaatac aaatgttgaa ccagtgtaat gaacacctgc
     5521 aaaatcgggg tgattaaaaa ttgtatcaga aatcatagca ggatctccaa aaactacatt
     5581 aataacacca tctggaacgc cagcttctct aaaaacatcg atgataattt ttgcagagaa
     5641 aacttgacta tcacttggtt tccaaacgac agtatttccc attaaagcag cacttgcagg
     5701 aagatttgcg gcaatagcag taaagttgaa aggagtaatg gcataaacaa atccttcaag
     5761 cggacggtat tcaacacgat tccaaacatc agaagtagat tttggttggt cttgataaat
     5821 ttgtgtcatg aattctacgt tgaaacgtaa aaagtcaatc aattcacaag aagcgtcgat
     5881 ttcggcttga aaaatagttt tagattgcgc aatcatggtt gcagcattta ttctagctct
     5941 gtatggtcca gcaataagtt cggctgcttt taaaaagata gcggctcttt gttcccaagg
     6001 catttgagac catttttttc tagcttctaa tgcttcaaca atagcttgat ctatatgttt
     6061 tttttctgct aaatgatagg ttccaacaat gtgttgatga tcatgtggtg cagacatgtt
     6121 atttgtattt ccagtgcgaa cttcttcttt tccaatatac atcggaactt caatagtttc
     6181 attccacatt tttctatatg cagccaatac ggctgttttt tctggagaat taggtgcgta
     6241 tgattttacg ggttcgttga ctgcttttgg gacattaaaa aaaccttttg gcatgatgtt
     6301 tattatttaa atagttagat aagttgataa ttataagcaa aataattgca gataatttaa
     6361 tttcacacaa aagtacaaag gtttttttga atttttagca cgatttaaat tcaatgctta
     6421 attcaatgca aaagcagcac tcaatttgaa tgttgggaca ataactctaa agtttcgtgt
     6481 agaagtaaag ttaatcatat taaaatgacc acgcatagct ccaaaaggcg atgaaagtaa
     6541 acaaccagaa ctataagtat gtaattctcc tggtttaaga actggttttt ttccgattac
     6601 tccttcgcca tcaacagttt ctgtgtcgtt taaagagtca aatatttccc aatgtcgtga
     6661 aagtaactga acagaatctt tgctgtggtt ttctatggta atttcataac taaaggcaaa
     6721 atgaatcttg tggttcttaa agtaagtacc ttcaaaacta gtagtaactg aaatttttat
     6781 gcctcttgtt atttgtgtaa ccatgaattg gttttgattt atttaggtca aaaatagtaa
     6841 aattttatag gttcaaaaac aatttagtgt taatttttag taagttatat ctaaagaaga
     6901 agcacttcct cttccaatta ctctatcata acgttggttg ttttctttgt aataaactaa
     6961 aactagataa ttgttttcgg ttactgcaaa gtttccatct attgcatttt cttcatcaag
     7021 atttccattg ccatctgaaa ttagatattg ataattggta aatccttgtt ttatcatgat
     7081 ggctttttca taaacgcctt ttttctcatt gtaaaccatt ttgttttcgt cgtttgtggc
     7141 ataattatta aacaagccat taacgtaaat agattttttc ccaaaataat ttggcgcact
     7201 aagagaaaag aaaacccatg cataatcagc ttcaatttca ttttgttctg cgccaatatt
     7261 gtttacaata aagtttccat tgaaatcagg aaagtaagta taaggtttgt ttgctctagc
     7321 ttcattggtg tatagataag agttataaac gccaccttga ttagaatcta ctcttgcaat
     7381 gttgttgttt gccgctcgaa tatctttatt ttcaaaaaac ataaattcgt ttccagccca
     7441 aaattgcgtt tctttatcgt atttataaat caagtcattt ccaatagtaa acattggctt
     7501 gatgtttttt attgcgttat caaaccgacc attttgtagt agcaatactt taacattttg
     7561 taaaggactt tgaaaattga tgttggatga ctttatggaa aattctaaat tttgtttgta
     7621 attcaacatc gaaacatctc ttgctctttt tacttctaat ggaacagaaa ccaaattttc
     7681 ataaacaatg aattttttag taaatactac atttttatca tcgtctaaga ttttgagaac
     7741 aaaatttcca ctaactcgca attgtgtaaa tttattagga agcgtgagtt tataatgaga
     7801 atacaattgt aacgtattta atgaattggt atagttttga atacgttgat cgtcaaaacc
     7861 gttaaggtat tctgcttttg ccaattgcga aggtttccaa tcgtaattac aatgggttaa
     7921 tgtgtagtag taatttgctt cattgccaaa taaatcatca aattgcaatt caaatgaatc
     7981 gcctaaagaa aaaacaggaa ttgtgttttg tgcgttttga acaaaagtca ctgttttgat
     8041 gttatatgga ggtgctattt ctttttcttg tgaaaaagca gttaagaaaa cgaataaaaa
     8101 aaggctagag aataatattt tttttgacat aataaacaaa tcgttttggt aaagatacta
     8161 aaaaatgtaa tctacaatta tgccaaaaaa atatttattt aaaacaaact ggaatgattt
     8221 cgccatttgc taaacaatat ttttcaacta attctggagc tattttagaa gtataatctt
     8281 cttcaattac tttaaatcca atacttctta gtttgtcaaa ataatctctt ccataaacgc
     8341 gaacgtgatc gtattgtcca aatattttgg cgcgttcttt ttggtcaata attgcatcat
     8401 ccgaaaaagt tgttgcccta gataagtctt gtggaatttg aaaaatgccc attccgttgg
     8461 gttttagtac gcgatatagt tcttgcattg ctttggtgtc atcaggaata tgttctaaaa
     8521 cgtgattgca aaaaataata tcataggtgt tatcttcaaa cggaagattg caaatatctg
     8581 cttttacatc agccaaaggc gaaagcaaat cagttgtagt ataatcgatg tttgtttgct
     8641 ttttaaaacg cttatagaat tcttgctcag gtgcaaagtg aagcactttt ttcttttctg
     8701 ttgtggtgaa aaaatcagtt tcattttgaa gatacaacca caataaacga tgtctttcta
     8761 gtgaaagcgt acttggtgaa agcacgttat ttctttgatt tccgtatcca taaggcaaaa
     8821 acattcgaaa actttttcca tcaataggat cggtaaaacg atttcctttt aataaaaaag
     8881 ctaaaatagg acgcactacg atgctcattc gtattaggat tggtcttggg atggtattta
     8941 gtatgaactt aaacacagat ttcacagatt ttcacagatt atattataaa attactcgtt
     9001 tgtatttaag gctatcttcg ccaaaattta gtaacaagcc taatttgttt ttagaacttg
     9061 ctaaatagtt taaagtttgt ttaatttcgc tatttgtcag ttctgaaatt gcttttactt
     9121 caaaaattat ttcatcataa actacaaaat cagcaatata ctttcttggt aaaataatgt
     9181 ctttgtattg tattttatag attttttctc tttcgtatgg aattgaattt attttaaatt
     9241 catattctaa tgcatcttta taaaccactt cactatgacc tttacccaga attttgtgaa
     9301 cttccataca aattccaatt atcttaaaag tttcctcttt taggtaaaaa ttctccataa
     9361 aaatctgtgt taatttgtgg aatttgtgtt tctaaactcg ttttcctcat cactttgaat
     9421 tcctagaaca gcatatatat attggaatgt agataataac tctggttttc cattaatcaa
     9481 agctacgtta tgttcaaaat gagcacttgg ttttccatcg cgggtaacga ttgtccattt
     9541 atcacttagg gtttttatgt tttttgtacc catattaatc attggctcga tagctacaac
     9601 cattccttca acaaatagtt ttccttttcc tcgtttgcca taatttggca tttcgggtgc
     9661 ttcgtgcatt tttcttccta aaccgtgtcc taccaattcg cgaactacgc cgtaaccaaa
     9721 actttcggta tatttttgaa tagcactacc aacatcttca acacgatttc cggctttaaa
     9781 ttcacgaatt ccaatgtaaa gactttcttt ggttacttga agcaactttt tggtttcagg
     9841 atttacatcg ccaatttcaa aagtataagc atggtcgcca taaaaatcat tcattaaaac
     9901 accacaatcg accgaaacca catcgccgtt ttttattggt ctgttggttg gtaatccgtg
     9961 aactacttgt tcgtttacac ttgttagcaa agtagatgga cagccatata aacctttaaa
    10021 tccaggtaat gctttgtgat ctctaataaa ttcttcagca aatttgtcta atgctaatgt
    10081 ggttacgcct tctttaattt cagcggctat catgcctaat gtttttgaaa ctaataatgc
    10141 gctttgacgc attaattcaa tttcttcggc agtttttgga ataatcataa attaatattg
    10201 agttcgcaaa agtagttatt ttaaaataat ttaatctgga tttgccatga ttttaaataa
    10261 ttcagcattg gagataaaga aacgattggc aacattaatt tttgataatt gactgctaac
    10321 taaattattt tctctagcat ttattaagaa aattgaactt tcaccaaaac taaaaagtcg
    10381 ttcttctgaa cgcaacatcg tttgatagtc gtttactaga ttttcgataa tatttatttg
    10441 tttgattaga gagttgattt ctgtttgttg tgcttttagt ttgtttttta attgcaatcg
    10501 tactactttt aaactgtatt cagcatcttg cactttgtat ttagctaatt ttaaactacc
    10561 acgttctttt ctcaaaaata aaggaaaaga aaaattaaca cctactttat agtcttcaaa
    10621 acgatatttt tcaaaatagc taggttcaga tagataggag tagcctaaat caattttagg
    10681 taataacaga tttgatttga gtttttgttc aatagtaagc atatcaattt tggtttgcca
    10741 agcattaatt tttggatggt tctctaagga tgcattttgt aacaaatcat ttgttcgcaa
    10801 cgtttctgat atagtacttt ctaatttatt ttctggaatt aaatcatctt gtaattctaa
    10861 gggtatatta ttgtctaacc ataaaaaatt agacaattct agtgtagctt tggctaattt
    10921 taattttgaa tcttcgagac ttaatgctct atttctaaca ataattccag cttcaatgct
    10981 gtctattgct ggtttgtcgc cttgctcgat agaaattttt attactttaa aacgagttgc
    11041 agcataagtt gcgtaatttt catataattt aacttcgtcg taattttttt tccaattaaa
    11101 atatgccaac gaagcattgt ataaaacagt tgttgcttct aaatttcttt cggcttggcc
    11161 tagcttgagt tgtatttttc cttttcgaac atcagccatt ctttgattaa taaacaaccc
    11221 ttgaccaact gaaactgcaa taccaaccga tgttaatcct tgatttggaa ctgtgttttc
    11281 gggatttaga tatactccat cattgttgtc aaaaccagct tttaattcaa ttccatacca
    11341 agttggtatt ttgaaactgc tgtttaaaat ggaataatat tcactgtctt taaatctttt
    11401 ttgttcataa tcaatttcga tttttgggtc gaaaccacct cgtgccatca tcaaagaagc
    11461 ttgcgcttca ctgatattta aatctgctaa ttttaccaaa ggatgatatt tttttacata
    11521 gcccaaatat tcattaaaag tgaattcatt ggaagctttg ttttgaccaa aaagattaaa
    11581 ccctaagagt aagaatatag aaagtaggag tttcattatt tttttgcttt atcagatttg
    11641 gggttataat aatttggcgg aaaaccattc aatgttctcc aaatttcaaa ccaaacggga
    11701 acattgtcta gcaatgctaa agtttgtgcg cctgatccaa tactaatttg ttttggccat
    11761 ggtgcttcag tttcatctgg agcaattaat actctgaatt taccgttttc gctaatgaaa
    11821 ttttcgatgg caactatttt tcctccaaaa gttccatatg acatatttgg ccagcctgaa
    11881 aatacaattg ttggccaacc atcaaaccat acgcgtactt tttcgccttt gtttattaat
    11941 ggcaaatcgt ttggattaac aaatgtttct acagcaattt cgtaatccga tggcatgatg
    12001 ctaactattt gtgttccttc tttaatcgtt tctccaattc ctgactgtag tgcacgattt
    12061 acaaaaccgc tttgcggtgc tttgatgtaa tacataccgt ttctgatttg gtaattctga
    12121 acttgatttt gcaatttgtt aacttgtgct ttggtgtcgt attgactact taatgcagta
    12181 tatttatcac ttgaagcttt ggattctttc tcattgtatt cagcgataat tctatttatc
    12241 tcaacttttg cattaatcaa ttcgttttca gcagtgatta atttgttttc ttgcgtaatg
    12301 attttagctt cagtttcttg tagttttaat cgtttttctt ctacatcagt cataggtttt
    12361 aagccttctt tgtttaatgt taacgatcga ttgaattgtg tgtttgcaat ttttatctgc
    12421 gttcgaacgg caactaggtc aatactatca cttttaattt ttaaaatggc ttgccgtact
    12481 ttgttttttg cttgttcaag ttttaatttt ttttcatttc taattgcttc aatttgagaa
    12541 gctaacgatt ttactttgct gccatacgat tcggtagcca tttctttggc ttgtagttga
    12601 ctttcagtat tttgtactaa atttgggtcg agataatctt cctttatttc agaaataaat
    12661 aaaatagtat cgcctttttt tacaaaatca ccttctttga cataccattt ttcaattctt
    12721 cctgcaattg cagtttgtac agtttgtggt ctttgatttg gttttaaagt agttacagcg
    12781 cctttgccag atatattttg tgtccatggc aaaaacaaaa tcaaaacacc taataatgta
    12841 aaccaaatga ttattcgatt taagatttta tagtgtggtc tgtttcttaa atctttaacc
    12901 gttttgtatt tgtcagtggt atctttaatg ctgttgtttt cagataagtt tagcattttt
    12961 atttttttaa atcgttgtaa atttctcctt cttgcattat tattattcga gtacattttt
    13021 tctcccaata gggatttttt gatgtaatta ctaaggccca tttctggtta ctatccgtaa
    13081 tgaaatctat taattcattt gccgttatat catccatttt atcaaatggt tcttctaaaa
    13141 ataaaatttt aggttttgtg atgatgcttc ttgctaataa aattttttgt gcattcgaag
    13201 aagaaagttg ttttccttcg ggatatattt ttgtgtccaa accttttggt aatgatttga
    13261 tatattctcc aagttgaaca ctgtcaatag cccattttaa atcttcttgg ctaatgtttg
    13321 gattgttaaa tgtaatattt tctaaaatgg ttccttcaaa aggtgtttct ccgctgataa
    13381 ttactgaaat ttgagatcta tattggtcta aatttatttt tctataagta tcgtcattga
    13441 tgaaaaatgt accagatgtt ggttgcaaca aaccagataa aattctaatt aacgtagttt
    13501 tacccgagcc attatttcct ttaataatta ttttttcgga aggttctagc ttaaagttaa
    13561 tcgtatttaa aacaaaatta tttgaatctt gaaacttata acacaatttt tctacttcca
    13621 aagtaatgtt ggaatagcaa atctcattag gaacgtccgt tgtattttca atttttaaat
    13681 ctgttatctg accaattttt tcaacggctg ttaaaacatc ataaagt
//
LOCUS       DAOOBH010000057        13663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5797_length_13663_cov_7.277484, whole genome shotgun sequence.
ACCESSION   DAOOBH010000057 DAOOBH010000000
VERSION     DAOOBH010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13663
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5797_length_13663_cov_7.277484"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="PK218_07045"
     CDS             <1..122
                     /locus_tag="PK218_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPW98297.1"
                     /translation="TVELIMEFEKEFDIQIPDDQAENIATVGQAISYIEEAKK"
     gene            291..1544
                     /gene="fabF"
                     /locus_tag="PK218_07050"
     CDS             291..1544
                     /gene="fabF"
                     /locus_tag="PK218_07050"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPW98298.1"
                     /translation="MTLKRVVVTGLGALTPIGNSVEEYWNALINGKSGAAPITYYDTE
                     KHKTKFACEVKNFNIEDFIDRKEARRMDKFAQYAVAASEEAIKDAGITDDNVNKHRVG
                     VIWGAGIGGLETFQEEVLSYAAGDGTPRFNPFFIPKMIADIAPAHISMRNGFMGPNYT
                     TVSACASSANALIDAFNYIRLGMCDVIISGGSEAAVTIAGMGGFNSMQALSTRNESPE
                     TASRPFDATRDGFVLGEGAGALVLEEYEHAKARGAKIYCEIGGGGMSSDAYHLTAPHP
                     EGIGVIAVMQNTLRDAGMTPDQVDHINTHGTSTPLGDVAELKAISAVFGDHAKDININ
                     STKSMTGHLLGAAGAIEAIASILAMKHGIVPPTINHEVVDERIDPTLNLTLNKAQKRE
                     VNVAMSNTFGFGGHNACVLFKKIVD"
     gene            1550..2290
                     /gene="rnc"
                     /locus_tag="PK218_07055"
     CDS             1550..2290
                     /gene="rnc"
                     /locus_tag="PK218_07055"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPW98299.1"
                     /translation="MGILKKIFSNSRSPEDGIFFSKMEKILGYKPVNIEYFQKAFTHR
                     SSNKIDGSGNPMNYERLEFLGDAMLGSVIAAHLYNQVPTGDEGYLTKMRSKIVSREHL
                     NELGRDLNLIQFVESKVPVSHFGENIHGNIFEALIGAIFLDKGYVACENFIMKKVIKP
                     YVDIPKLEGKVISYKSLLIEWCQKEKRTFNFEVYDDNGNDGQKFFGVKLSIDGKIIAK
                     ARATSKKKAEEKASQRAYFVFQEKINNK"
     gene            2402..2893
                     /locus_tag="PK218_07060"
     CDS             2402..2893
                     /locus_tag="PK218_07060"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HPW98300.1"
                     /translation="MMVTYKLFSEEIEEVDYQLIAIHTTIEDFRLAYFINKNLSISLS
                     KHPEDLSIQDKNREIYFSRFYFEDDNNSISWNLIQNKSEDDQNMATLSTGLFADSISR
                     VSTKTHLIPEYKRVDYFLKVDKSFTDAQIQETISKIKKIQKVSTVYTIDAEKIKSKNN
                     LIF"
     gene            2896..4326
                     /gene="pyk"
                     /locus_tag="PK218_07065"
     CDS             2896..4326
                     /gene="pyk"
                     /locus_tag="PK218_07065"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPW98301.1"
                     /translation="MSTRKKTKIVATLGPACSSKEVIKEMIDAGVNVFRINFSHADYE
                     DVKERIGIIRGLNDEFGYTTAILADLQGPKLRVGVMKEDVVVNPGDMITFQTAEDVPG
                     TAKRVYMNYKEFPKDVNPGEKILLDDGKLMFEALETNGTTEVVCKVIQGGPLKSKKGV
                     NLPNTKVSLPALTKKDIKDALFAIKQEVDWIALSFVRTPKDLEELQDLIAKHSSYKIP
                     IIAKIEKPEAVENIDKIVAFCDGLMVARGDLGVEIPAHEVPLIQKKLIHRAKTARIPV
                     IVATQMMETMITSLTPTRAEVNDVANSVMDGADAVMLSGETSVGNYPVQVIEKMTQII
                     EAVEDSPLIVVPQNPPHVRTKRFITKSICYHAAIMANEIKAKAISTLTNSGYTAFQIS
                     AWRPQAHILVYTSNKRILTQLNLLWGVNAFFYDKFVSTDDTVDDINLIAKDKGFVKNG
                     DMLVNLAAMPVANKGMVNTLRISEIE"
     gene            4367..5125
                     /locus_tag="PK218_07070"
     CDS             4367..5125
                     /locus_tag="PK218_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HPW98302.1"
                     /translation="MESKNPFFKNKTFNNTSREVTHQATVIDYNESMTVSGTINKSFL
                     MLLLLVASAAITWMMFFNGYNPMVFTIGGAIVGLILVIISAFKPHLSPYLAPGYAVFE
                     GLFIGGISAIFESMYPGIVIQAVSCTFVTFMVCFGLYKYEIVKVTEKFRSVVIAATLA
                     IATYYIITWLLSMFTSFQPVHYGNSWVSIGISAFVIVIAALNLFLDFDQIEKGVQQKI
                     PKYMEWYGAMGLMITLVWLYIEFLRLLSKISSRD"
     gene            complement(5212..>5688)
                     /locus_tag="PK218_07075"
     CDS             complement(5212..>5688)
                     /locus_tag="PK218_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98303.1"
                     /translation="GCRNNPNLTSINIRNGMTQAFGPGTYYNQCWSNLPNLSYICADG
                     DEIPALQSFLTGCGVTQNITIDSACPLSVNEYELNSLIITPNPSNGLFQLSFASKLPQ
                     EVVYAVYDMLGKRLIGKKVSFENNSIEINLVDYPAGVYQLRISLGETVVNKKLVKK"
     assembly_gap    5689..5788
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6061..6720)
                     /locus_tag="PK218_07080"
     CDS             complement(6061..6720)
                     /locus_tag="PK218_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98304.1"
                     /translation="MNSYVRIEEVAPLGDFVKTSYTRDFDAIKSKFPKMDDAFKTNFF
                     DKLEFVKELESSLELTESQKGVTTSLYAEADALNEELNFLSAYFDDAGLNKGIITDLK
                     TDLFNGNIEGAILKIEGLKQFVTIHQVDLIAEGMEANYVTRLGDYKTSMTAKNVLQNE
                     IMNNRKELTERNVGHYDELKKMIRKILRNGKLVFANSVIKDEYTSSKVLQRMRAARAK
                     G"
     gene            complement(7221..8303)
                     /gene="dinB"
                     /locus_tag="PK218_07085"
     CDS             complement(7221..8303)
                     /gene="dinB"
                     /locus_tag="PK218_07085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPW98305.1"
                     /translation="MEEPVSYRKIIHIDMDAFYASVEQMDNPELRGKPLAVGGSEIRG
                     VVSAASYEARKFGVRSAMSGVQAKRNCPELIFVRPRFDRYKEISKKIRKIFHDYTDLV
                     EPLSLDEAYLDVTQNKKGNPSASLLAQEIRQRIFDEVGLTASAGISVNKFVAKIASDY
                     NKPNGQKTVTPEEVEAFLEGLDIKKFYGIGKVTAEKMYQLGIFTGKDMKAKTAAFLEQ
                     HFNKSGLHYYNIVRGIHNSPVKPNRITKSVAAEHTFNENLTSEIFMLEKLERIAGELE
                     KRLRKHKISGKTVTLKIKYSDFTQQTRSKTLPYFISDKGILLETAKELLYQERMKESV
                     RLLGISLNNLNTEIKKTVVVQLKFEF"
     gene            8345..8812
                     /locus_tag="PK218_07090"
     CDS             8345..8812
                     /locus_tag="PK218_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPW98306.1"
                     /translation="MIEIERKFLVTSKNFINEAFSKKRIVQAYLSSVPERTVRIRIKD
                     DKGFLTIKGKSNETGTSRLEWEKEIDVQEAEKLLSICEKGMIDKIRYEVKSGKHTVEV
                     DVFSGENEGLIIAEIELEDENELFEKPSWLGKEVTNDERYYNVYLSQKPFKDW"
     gene            complement(8813..9163)
                     /locus_tag="PK218_07095"
     CDS             complement(8813..9163)
                     /locus_tag="PK218_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPW98307.1"
                     /translation="MQLLDSIKKKQEFNILKLHKKNAHASYYHTKFNGRRTASGEIFD
                     NEKYTAAHKTLPFGTMLKVTSETTGRFVIVKVNDRGPFVKARDIDLSRKAFMDLVDNK
                     GRGTVPVTIEIIEE"
     gene            complement(9426..9848)
                     /locus_tag="PK218_07100"
     CDS             complement(9426..9848)
                     /locus_tag="PK218_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98308.1"
                     /translation="MYQFVQKFHSGWAYLAVLLLLFAALNAVIGLTSKKEFTPKDRKI
                     SLFGLLATHIQLLVGLILYFVSPLGFNSLGNMKDAAIRLTSLEHPLINIIAIALITIG
                     WSRHKKATTSEAKFKAITLFYGIGLLLILSRIPWYLWL"
     gene            complement(9924..11567)
                     /gene="pgi"
                     /locus_tag="PK218_07105"
     CDS             complement(9924..11567)
                     /gene="pgi"
                     /locus_tag="PK218_07105"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPW98309.1"
                     /translation="MSLSSINPTKTEAWKKLLAHFAITKNNTMQELFKNDATRADKFH
                     IQWKDFLVDFSKNRIDSTTVKLLFELAEECNLKEAIDSYFKGDAINKTENRAVLHTAL
                     RAPKNATVLVDGINVIPEIYAVKEAIKTFTNEIVLGNKKGYTDKVFTDVVNIGIGGSD
                     LGPAMVVESLQYYKNHLNVHFVSNVDGDHVNEIIKKINPETTLFVIVSKTFTTQETLS
                     NAETIREWFLQSATQADVAKHFVAVSTNLQKVTEFGINPTNVFPMWDWVGGRFSLWSA
                     VGLTISLAVGYENFEALLEGAHEMDVHFQETKFEENIPVVLALLSIWYNNFYGAESEA
                     MIPYTQYLQKLAPYLQQGIMESNGKSINRDGKSVTYQTGTIIWGEPGTNSQHAFFQLI
                     HQGTKLIPTDFIGYLKPLYGNKNHHDKLMSNFFAQTEALLNGKTAAQVQAEFDKANMP
                     SAQAAFLLPFKVFEGNKPTNTLLIDQLTPRSLGALIALYEHKIFVQGVIWNIYSYDQW
                     GVELGKQLANSILAEIETKTIKTHDSSTMFLLKHFLKNT"
     gene            complement(11656..12894)
                     /locus_tag="PK218_07110"
     CDS             complement(11656..12894)
                     /locus_tag="PK218_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPW98310.1"
                     /translation="MLKTVQRLLLLLFTTVLLFSCKKDNEIQIVTLPVKEKTIDFGFN
                     LDAFNVVHDTIKRGDTFGTILDHQNLGGNQVYDIVAKVKDTFNVRSIRVGKPFTFLRT
                     KDKKHRLQYFIYQPDKLTYYVIDLRDSVMAYKKTRPLTFKSRTIAGALNGSLSETLQN
                     LKVDPSLASRIAKIYAWSIDFFKLQKGDEFALSFTERFINDTVYDGVDSLKAAFFEYK
                     GKKIYAFPFSTDGSKGKLQYFDEEGKTLKNFFLKAPLKFVNITSRYTKRRFHPVQKRW
                     KAHNGTDYAAPTGTPIMTTAAGVVIQAGYTTGNGNYVKVQHDKTYTTQYLHMSKILVK
                     RGQRVTQGQVIGKVGSTGLATGPHVCYRFWKNGVQVDALRLNLPTSTPMDAKYKQAFM
                     QQMTPLKKELDSIAKTIEKK"
     gene            complement(12904..>13663)
                     /locus_tag="PK218_07115"
     CDS             complement(12904..>13663)
                     /locus_tag="PK218_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HPW98311.1"
                     /translation="LPDEMVFIMYHQVNELLFKMILWEIDQLCHTDKPETAYFTEKLG
                     RISRYFDMLTTSFDIMGDGMEVAQYMKFRNTLTPASGFQSAQYRMIEFASTDLINLID
                     YRFRATIDRDTPYTHAFEHLYWQAAGKDHATGKKSYLLLAFEEKYKDEFLRFMEEYNT
                     INVWQKFKQLPEDDQKNAELISAMRHYDYTVNITWVMGHLNAARKYIDSGQGTGEATG
                     GSDWKKYMHPKYQRRIFFPALWSEEELKNWGEGL"
BASE COUNT         4436 a         2469 c         2385 g         4273 t
ORIGIN      
        1 acactgttga gcttattatg gagttcgaaa aagaatttga tattcaaatt ccagatgatc
       61 aagcagaaaa tattgctact gttggtcaag ctatttctta cattgaagaa gctaaaaaat
      121 aataattaaa aatacccgta gcatttttgt tacgggtatt tattattttt aaaaacatct
      181 actcagattg ttttcaatct aaaaaatatt atacccatgt ctctttaatg cttatatttg
      241 aagaatacat gggtatgttt ttataaaaaa ggagttcaaa aatagaatat atgacattaa
      301 aacgagttgt agttacaggt ttaggagcat taacaccaat aggtaattct gttgaagaat
      361 attggaatgc attgataaat ggtaaaagcg gtgccgcacc aataacctat tatgatactg
      421 aaaaacataa aaccaaattt gcttgtgaag taaaaaactt caatattgaa gattttattg
      481 atcgcaaaga agctcgtaga atggataaat tcgctcagta tgccgtggca gctagtgaag
      541 aagctattaa agatgcaggt attactgatg acaatgtgaa taaacacaga gttggcgtaa
      601 tttggggagc aggaattgga ggattagaaa cttttcaaga agaagtattg agttatgctg
      661 ctggtgatgg aacacctagg tttaatccat tttttattcc aaaaatgatt gctgatattg
      721 ctccagcgca tatttcaatg cgaaacggat ttatgggacc aaattacaca acagtttctg
      781 cttgtgcttc gtctgctaat gccttgatag atgcattcaa ttacatccgt ttaggaatgt
      841 gtgacgttat tatatctggt ggttcggaag ctgcagttac tatagctgga atgggagggt
      901 ttaattctat gcaagcttta tccacaagaa atgaaagtcc ggaaacagct tcaagaccat
      961 ttgacgccac gcgcgatgga tttgttcttg gcgaaggtgc cggtgctttg gttttggaag
     1021 aatatgaaca tgccaaagca cgtggcgcaa aaatatattg cgaaattggc ggcggcggaa
     1081 tgtcatcgga tgcttatcat ttaacggcac cacatcctga gggaattggc gtaatagcag
     1141 taatgcaaaa taccctgcgc gatgccggaa tgacaccaga ccaagttgac cacattaata
     1201 cacatggaac ttcaacacct cttggggatg ttgccgaatt gaaagcaata agtgctgttt
     1261 ttggcgatca tgctaaagat attaatatta attcaactaa gtccatgaca ggtcatttgc
     1321 ttggagcagc aggtgctatt gaagcgattg cctctatatt agcgatgaag cacggaatcg
     1381 ttcctccaac aatcaatcac gaagtagtag atgaacgaat tgatccgact ttgaatttaa
     1441 ccttgaacaa agcgcaaaag agagaagtaa acgtcgctat gagtaacact tttggttttg
     1501 gaggacacaa tgcttgtgta ctattcaaaa aaatagttga ctaactagga tgggtattct
     1561 caaaaaaata ttttcaaatt cccgttcgcc agaggacggg attttttttt ccaaaatgga
     1621 gaaaatattg ggttacaaac ctgttaacat agaatatttt caaaaggcat ttacacatcg
     1681 ttcttctaat aaaatagatg gtagcggcaa tccaatgaat tatgagcgtc tggaatttct
     1741 gggtgacgcc atgctaggct ctgtaattgc tgcacattta tacaaccaag tgccaacagg
     1801 cgacgaaggc tatttgacca aaatgcgctc caaaattgtg agtagagagc acttgaatga
     1861 actcggtaga gatttaaatt tgattcaatt cgtagaaagt aaagttccag taagtcattt
     1921 tggtgaaaac atccatggta atatttttga agcattaatc ggtgcaattt ttttagacaa
     1981 aggttatgta gcctgcgaaa attttatcat gaaaaaagtg attaaaccat atgttgatat
     2041 tcctaaacta gaaggcaaag tgattagtta caaaagtttg ctgattgagt ggtgtcaaaa
     2101 agaaaagcga accttcaatt ttgaagtcta tgatgataat ggaaatgatg gtcagaaatt
     2161 ctttggcgta aaattatcca ttgatggtaa aataattgca aaagcaagag ctacttctaa
     2221 gaaaaaagca gaagagaaag cttcgcaacg ggcatatttt gtttttcaag aaaaaattaa
     2281 caataaataa tcacaatcca caagactata tcgatttagt tcataaaatt acgttaatca
     2341 taaaaattac tccctaaaat atgcggtaga tatagtatat ttaccaatca tttgcgttga
     2401 tatgatggtt acatacaaat tgtttagcga agaaatagaa gaagtagatt accaactaat
     2461 tgctattcat accaccattg aagattttag attagcctac tttatcaata aaaatctatc
     2521 catcagttta tcaaaacacc ctgaagatct tagtattcaa gataaaaata gagagatata
     2581 tttttctaga ttctattttg aggacgataa caattccatt tcctggaatt tgattcaaaa
     2641 caaaagcgaa gacgatcaaa acatggcaac gttaagtaca ggcttgtttg ccgatagtat
     2701 ttcgagagtt tccacaaaaa cacatttaat ccctgagtat aaaagagtag attattttct
     2761 aaaagtagat aaatctttta ccgatgctca aattcaagaa acaatttcta aaataaaaaa
     2821 aatacaaaaa gtaagcacgg tatatactat tgatgctgaa aaaataaaat caaaaaacaa
     2881 tttaattttt taataatgtc aaccagaaaa aaaacaaaaa ttgtagccac attaggtcct
     2941 gcatgtagtt ccaaagaagt tattaaggaa atgatcgatg ctggggtaaa cgtatttcgt
     3001 atcaattttt ctcatgccga ttatgaagat gtaaaagagc gaataggtat tatcagaggc
     3061 ctgaatgacg agttcggata cacaactgct atccttgctg acttacaagg cccaaaactt
     3121 agagtaggtg taatgaaaga agatgtagta gtaaatccag gtgatatgat tacctttcaa
     3181 actgctgaag atgttccggg aactgccaaa cgtgtctata tgaactacaa agagtttcct
     3241 aaagatgtaa atccaggaga aaaaattctt ttagacgatg gaaaattaat gtttgaagca
     3301 ttggaaacca acggaactac agaagtagtt tgtaaagtaa ttcaaggcgg accattaaaa
     3361 tctaaaaaag gagtaaatct tccaaatacc aaagtttctc ttccagcttt aaccaaaaaa
     3421 gatattaaag atgctttgtt tgctatcaaa caagaagtag attggattgc actttctttc
     3481 gttagaacac caaaagattt agaagaatta caagatttaa ttgcaaaaca ttcttcgtat
     3541 aaaattccaa ttattgctaa aattgaaaag ccagaagccg tagagaatat tgataaaata
     3601 gttgcgtttt gtgacggttt gatggtagct cgtggcgatt tgggcgttga aattccggcg
     3661 cacgaagtac cattaattca aaagaaatta attcacagag ccaaaacggc acgtattcct
     3721 gttattgtgg caacacaaat gatggaaacc atgattacta gcctaacacc aacacgtgct
     3781 gaggtaaacg acgtagccaa ttcggttatg gatggtgctg atgcggtaat gctatctggc
     3841 gaaacatcag tgggtaacta tccggttcaa gtaatcgaaa aaatgactca aatcatagaa
     3901 gctgttgaag attcaccact tattgttgtg ccacaaaatc cgcctcatgt tagaacgaaa
     3961 cgatttatta ccaaatcaat ttgttatcac gcagccatta tggcaaatga aatcaaggca
     4021 aaagccattt ctacgcttac gaatagtggt tatactgcct tccaaatttc agcttggcga
     4081 ccacaagctc atattttggt atatacttcc aataaaagaa ttttgacaca attaaatttg
     4141 ctttggggag ttaatgcttt tttctacgat aagtttgtga gcaccgatga tacagttgat
     4201 gatattaacc ttattgcaaa agacaaaggc tttgtaaaaa atggcgacat gttagtcaat
     4261 cttgctgcga tgccagtagc caataaagga atggtaaata cgttgcgtat ttctgaaatt
     4321 gaataaaaaa aactattttt gataaaacta tttaatataa aagacaatgg aatctaaaaa
     4381 tccgtttttt aagaacaaaa ccttcaacaa tacttctcgc gaagtaaccc atcaagcaac
     4441 tgtcatagat tataacgaat caatgaccgt atcaggaaca ataaacaaaa gtttcttaat
     4501 gttgttgcta ttagtagcca gtgctgccat tacttggatg atgtttttca atggatataa
     4561 cccaatggta tttaccatcg gtggtgccat cgttggatta atcttagtaa taatatcagc
     4621 cttcaaaccg catctttcac cttacttagc gccaggttat gccgtattcg aaggattgtt
     4681 tattggtgga atatccgcta tttttgaatc gatgtatcca ggaattgtga tacaagccgt
     4741 aagttgcacc tttgtaacct ttatggtgtg ctttggacta tacaaatacg aaattgtaaa
     4801 agtaaccgaa aaattccgct ctgtagttat tgccgctact ttagccattg ctacttatta
     4861 cataattact tggttgctat cgatgtttac aagctttcaa cccgttcatt acggaaactc
     4921 ttgggtaagc atcggaatca gtgcttttgt aattgtaatt gcagccttaa acttattctt
     4981 agactttgat caaatagaaa aaggcgtaca acaaaaaata cccaagtata tggaatggta
     5041 tggcgcaatg ggcttaatga ttacattagt ttggttgtat atcgaattcc tgagattatt
     5101 atctaaaatt tcgagtagag attaatttca atttaaagaa aaaacaaatc gcctaaagtt
     5161 tcctttaggc gatttttgtt ttaagtgaat tctaattact aaaaaccaga tttatttctt
     5221 caccaacttc ttatttacaa ccgtttctcc aagactaata cgtaactgat aaactccagc
     5281 aggataatca acaaggttga tttcaatact attattttca aacgacactt tctttccaat
     5341 aagccttttg cctaacatgt cataaacagc atacactact tcttgtggta atttagaagc
     5401 aaagcttaac tgaaataaac cattactagg attaggagta attattaagg aattcaattc
     5461 atattcattt acagaaagcg gacaagccga atctatagta atattttgcg taacaccaca
     5521 acctgtcaag aaactttgca atgcaggtat ttcatcacca tcagcacaga tgtaacttaa
     5581 attaggaaga ttactccaac attggttgta atacgttcct ggaccaaatg cttgagtcat
     5641 tccgtttctt atgtttatag aagtcaggtt tggattattc ctgcaaccnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca attatccatt gaacttccga tgcatgcatt
     5821 gccattagaa gcatttgctc ctaataatct ggctttaaaa tttgcatccg gaatagtaat
     5881 gatttgagca ttgcaaaaac catatattaa aaggaataaa agacttatgt ttttcacagt
     5941 tattttttta tgatgttaac caacttcaaa acaaatctaa agaaaaaaca attgcatacc
     6001 acaaaaaaac ccagcaacat cgtcactggg ttcaaataac caaaaaacca aatccacaca
     6061 ttaacctttc gctctagcag cacgcatcct ttgtagcact tttgaactag tatattcatc
     6121 tttgataacc gagttagcaa aaaccaactt accattgcgc aatattttcc gaatcatttt
     6181 tttcaattcg tcataatgac caacattacg ctccgtcagc tctttgcggt tgttcataat
     6241 ttcgttttgc aaaacatttt tcgccgtcat acttgtcttg taatctccca atcgtgtaac
     6301 ataatttgct tccattcctt ctgctatcaa atcaacttga tgaatagtaa caaactgttt
     6361 tagaccttca attttcaaaa tagcaccttc aatattacca ttaaacaaat ccgttttcaa
     6421 gtccgtaata atgcctttat tcaacccagc atcatcaaaa taagcactca aaaaattcaa
     6481 ctcctcattc aaagcatccg cctcggcata caaacttgtc gtaactcctt tttggctctc
     6541 tgtcaactcc aaagagcttt ccagttcctt tacaaattcc aacttatcaa aaaaattggt
     6601 tttaaaagca tcatccattt ttggaaattt gctttttata gcatcaaaat cacgagtata
     6661 actcgttttt acaaaatctc caagcggagc aacctcttca attctaacat aactgttcat
     6721 gattaaataa tattaaatta aactttgagt gaagcgctta caagcagtta agcacaaaaa
     6781 tcaaaacaca aatccaatgc caaaaacaaa tgaaaaacaa tgctttaaca aatatttaac
     6841 caaaacaatt tacatagttt ttcaaacagt cgccttagta aaatgcacgt tgtgaacttc
     6901 gtgccttctt tgtgcctttg tggttaaaaa aacaacacaa atcaaatttc aaaaaagatt
     6961 ttagttgtga taaaagagcc aaacaaaata tccaataagc taaataaaac atccagtaag
     7021 ctaaatagaa tacccagaca gctaaataga gtttccaata agctaaatag aatacccaga
     7081 cagctaaata gagtttccag taagctaaat agaataccca gacagctaaa tagaatatcc
     7141 agatggctaa atagaacttc caataagcta aatggaatag tcaataaaaa taaaagaaga
     7201 aaaagaaacc aaaaaccaat ttaaaactca aacttcaact gcaccactac agttttttta
     7261 atctcggtat tcaaattatt caacgaaatg cccaataatc gaacactttc tttcatccgc
     7321 tcttgataca atagttcttt ggcggtttcc aataaaatcc ctttatccga aataaagtaa
     7381 ggcaaggttt tgcttcgagt ttgctgcgta aaatcgctat attttatctt taacgttaca
     7441 gttttccccg atattttatg ttttcgaagg cgtttttcaa gttccccagc aatgcgctct
     7501 agcttttcca gcataaaaat ttccgaagtc aagttttcat taaaagtatg ctcggcagca
     7561 acagatttgg ttattcgatt gggttttact gggctgttgt gtatcccacg aacaatattg
     7621 tagtaatgca aaccgctttt gttaaaatgt tgctctaaaa aagcagccgt tttagccttc
     7681 atgtcttttc cagtaaaaat gcccaactga tacattttct cagccgtaac cttgccaata
     7741 ccatagaatt tcttaatgtc taaaccttcc aaaaaagctt ccacctcttc aggagtaacg
     7801 gttttctgtc cgttaggttt attataatca ctagcaatct ttgccacaaa cttattgaca
     7861 gatattcctg ccgaagccgt caaacccact tcatcaaaaa tgcgttgcct aatttcttgt
     7921 gccaataacg aagcacttgg gttgcctttt ttattctgag taacgtcaag ataagcttca
     7981 tctagtgata gcggttctac caagtcagta tagtcgtgaa aaatctttct aatcttcttc
     8041 gaaatctcct tataacggtc aaaacgagga cgaacaaaaa tcaactcagg acaatttcgc
     8101 ttcgcctgaa cgccactcat agcactgcga acgccaaact ttcgcgcttc ataactagca
     8161 gcagaaacaa cacctcgaat ctcgctacca ccaacagcca aaggctttcc tcttaattca
     8221 ggattgtcca tctgctccac agaagcataa aaagcatcca tgtcaatgtg aataattttg
     8281 cgatatgaaa ctggctcttc catttcacaa atttatacta tatttagctt ctcaaaaatt
     8341 tcaaatgata gaaatagaac gaaagttttt agtcacttcc aaaaatttta taaacgaagc
     8401 cttctctaag aaaagaatcg tgcaagccta cctgtcatcg gttcccgaaa gaacagtgcg
     8461 catcagaata aaagacgaca aagggttttt aaccataaaa ggaaaaagta acgaaaccgg
     8521 aacgtcacgg ctcgaatggg aaaaagaaat tgatgttcaa gaagccgaaa aactactttc
     8581 tatttgcgaa aaaggcatga tagataaaat tcgttatgaa gttaaaagcg gaaaacacac
     8641 cgtagaagta gatgttttct cgggcgaaaa cgaaggatta attatagctg aaattgaatt
     8701 ggaagacgaa aacgaattgt ttgaaaaacc ttcttggtta ggcaaagaag taaccaatga
     8761 tgaacgttat tacaatgtct atttgtctca aaaacctttt aaagactggt agttattctt
     8821 caataatctc aatcgttaca ggaaccgttc cacgaccttt attatctact aaatccataa
     8881 atgctttgcg agataaatcg atgtctcggg cttttacaaa tggcccacgg tcgtttactt
     8941 taacaattac aaagcggccc gttgtttcac tcgtcacttt aagcattgtt ccgaaaggca
     9001 atgttttgtg cgctgccgta tatttttcgt tatcaaagat ttcaccactt gcagttcttc
     9061 ttccattaaa tttagtgtga taatacgaag cgtgtgcatt tttcttgtgc aatttgagga
     9121 tattgaactc ttgttttttc ttaatcgaat ccagtagttg aatggcagct gttttcaatg
     9181 gagtaatcgt actatctact ttggtttcct taataaactt atctgttttc ttaacggtag
     9241 caatggtagt tttgccttta ttcttttctt tttgttgtgg ggttcccaaa gtaaatgccg
     9301 atagcgacgt agcaattact agcaaaaaaa taaagaagaa cagtttagta cgcatgtgta
     9361 taattttttt agggagaagt acaaaaactg tacctcgata aaaaatcctt ttcaagactt
     9421 tatttttata accatagata ccaaggaatt ctacttaaaa taagcaacaa acctattccg
     9481 tagaacaatg tgatggcttt aaacttggct tcgcttgtag ttgctttttt atgtctagac
     9541 caaccaatag tgattaatgc aatggcaatt atattgataa gtggatgctc taaagaagtt
     9601 agtctaatag ccgcatcttt catgtttccc aaagaattaa aacccaacgg agatacaaaa
     9661 taaaggatta aaccaaccag taattgaata tgagtagcaa gcaatccaaa caaggaaatc
     9721 tttctgtctt ttggagtaaa ttcttttttg gaagtaagtc caataacagc attaagtgct
     9781 gcaaaaagta ataataacac ggctagataa gcccaacccg aatggaattt ttgtacaaac
     9841 tgatacatag ttaaattgtt ttaaacacaa atataactaa aaggcataaa aaaacgacac
     9901 tttaaagtgt tgtttagtca attttaagta ttctttaaaa agtgtttcaa taaaaacata
     9961 gtggacgaat catgggtttt gattgttttt gtttcaatct ctgccagtat cgagttggcc
    10021 aactgctttc caagctcaac accccattgg tcgtaactgt atatgttcca aataacacct
    10081 tgaacaaata ttttgtgttc atataaggca attaacgcac ctaacgatcg tggtgttaat
    10141 tgatctatta aaagagtgtt ggttggtttg tttccttcaa aaaccttgaa aggaagaaga
    10201 aacgctgctt gtgccgatgg catgtttgct ttatcaaatt ctgcttgtac ttgtgctgcg
    10261 gttttaccgt ttaataatgc ttctgtttgt gcaaagaaat tagacattag cttatcatga
    10321 tgatttttat taccatacaa aggcttcaaa taacctataa agtcggttgg gatgagcttt
    10381 gttccttggt gaattagttg aaaaaaagcg tgctgcgagt tggtcccggg ttcaccccaa
    10441 atgattgtgc cagtttgata ggtaacggat ttgccatcac gattaatgct ttttccgtta
    10501 ctttccatga tgccttgttg aagataaggt gctaattttt gtagatattg cgtataagga
    10561 atcattgcct cactttctgc accataaaaa ttattgtacc aaatgcttaa caacgccaaa
    10621 acaaccggaa tgttttcttc gaattttgtt tcctggaaat gcacatccat ttcatgtgcg
    10681 ccttcaagta atgcctcaaa attttcataa ccaacagcca agctaatcgt taaaccaact
    10741 gcactccaaa gtgaaaatct tccgccaacc caatcccaca tagggaaaac attggtgggg
    10801 ttgatgccaa attcggttac tttttgaagg ttggttgaaa ctgccacaaa gtgtttggca
    10861 acgtctgctt gtgtggccga ttgtaaaaac cactcacgaa tggtctctgc attcgacaag
    10921 gtttcttgcg tggtgaaggt ttttgaaaca atgacaaaca acgttgtttc tggatttatt
    10981 tttttgataa tctcattcac atggtcgcca tctacgttgg aaacaaagtg aacgtttaaa
    11041 tggtttttgt agtattgtaa cgattctaca accattgccg gtccaaggtc agagccgccg
    11101 atgccgatgt ttacaacatc agtaaacact ttatctgtat aacctttctt gttgcccaaa
    11161 acaatctcgt ttgtaaacgt tttgatggct tcttttacgg cataaatttc agggataaca
    11221 ttgatgccat caactagtac ggttgcgttt tttggtgctc gcaaagccgt gtgaagtact
    11281 gctcggtttt cggttttatt aatcgcatcg cctttaaaat agctgtcaat ggcttctttt
    11341 aggttacatt cttcggctag ttcaaataaa agttttacgg tagtgctatc tattctgttt
    11401 ttggaaaaat caactaaaaa gtctttccat tggatatgaa atttgtctgc tcttgtagca
    11461 tcatttttaa acaactcctg catggtgttg tttttggtaa tggcaaagtg tgccaaaagt
    11521 ttcttccagg cttcagtttt tgtagggttt attgaggata aagacatgtt tctagtattt
    11581 gaaaaatcaa atttacaaat aaagaagcgg aactcataaa agaaatatta cgctaacgtt
    11641 gtagtgctga aaattttatt ttttttcaat cgtctttgca atactatcca actctttttt
    11701 gagaggtgtc atttgttgca taaacgcttg tttgtattta gcgtccatag gagtagaggt
    11761 tggcaggttt aagcgcaagg catctacctg aacaccattt ttccaaaagc gataacaaac
    11821 atgaggacca gttgctaaac cagtactacc tactttccca ataacttgtc cttgtgtaac
    11881 gcgttgtcca cgttttacta agattttaga catgtgtagg tattgggttg tataggtttt
    11941 gtcgtgttgc acttttacat agtttccgtt tccagtggta taacccgcct ggataacaac
    12001 tcctgcggca gtagtcataa ttggcgttcc ggtaggtgcg gcatagtctg ttccgttgtg
    12061 tgctttccag cgtttctgaa ctgggtgaaa ccttcttttg gtatatcttg aggtgatgtt
    12121 tacaaacttt aacggtgctt tgaggaaaaa gttcttcagt gttttccctt cttcgtcaaa
    12181 gtattgcaac ttgcctttac taccatcggt tgaaaatggg aacgcatata ttttcttccc
    12241 tttgtattca aagaaagctg ccttcaaact atctacaccg tcataaacag tgtcattgat
    12301 aaatctttcg gtaaacgaga gcgcaaactc atcacctttt tgtagtttaa agaaatcaat
    12361 agaccatgca taaatctttg caattcttga agccaatgat ggatctactt ttaggttctg
    12421 taaggtttcg gatagcgagc cgtttaaagc accagcaatg gttcttgatt taaacgttaa
    12481 tggacgggtt tttttatagg ccatcaccga atccctcaag tcaattacat agtaggtgag
    12541 tttgtcaggt tggtagataa agtattgaag gcggtgtttt ttgtctttgg ttctaagaaa
    12601 ggtaaagggt ttaccaacgc ggatgcttct tacgttaaaa gtgtctttta ctttagcaac
    12661 aatgtcatac acttgatttc cacccaaatt ttgatggtct aaaatggtgc caaatgtgtc
    12721 gcctctttta attgtatcgt gtaccacgtt aaaggcatct aagttaaatc caaaatcgat
    12781 ggttttttcc ttaacaggta acgttacaat ttggatttcg ttatcttttt tacaagaaaa
    12841 taataataca gtagtaaata atagtaaaag tagtctttga acggttttta acatccttaa
    12901 tttttataag ccttcgcccc aattttttaa ttcttcttca ctccacaaag ccggaaagaa
    12961 tatccgcctt tggtattttg ggtgcatata cttcttccaa tcgcttcctc cagttgcttc
    13021 tcctgttcct tgtccactgt cgatgtactt cctagctgcg ttcaaatgtc ccattaccca
    13081 ggtgatgtta accgtgtaat cataatggcg catagcactt atcagttcgg catttttttg
    13141 gtcatcttct ggcaattgtt tgaatttctg ccaaacattt attgtattat attcttccat
    13201 aaagcgaaga aactcgtctt tatatttttc ttcaaaagcc aagaggaggt aggacttttt
    13261 acctgttgca tgatctttcc ctgctgcttg ccaatacaaa tgctcaaacg catgcgtgta
    13321 gggtgtgtct ctatctattg tagctctaaa tcggtagtct atcaaattaa tcaaatcggt
    13381 agatgcaaac tctatcattc ggtattgcgc actctgaaaa ccactagcag gcgttaaggt
    13441 gtttctgaac ttcatgtatt gagctacttc catgccatca cccatgatgt cgaatgatgt
    13501 tgttagcata tcaaagtagc gactgattct tcctagcttt tcagtaaaat aggctgtttc
    13561 tggcttatcg gtatgacaca attggtctat ctcccaaaga atcatcttaa acaacaattc
    13621 gtttacttga tgatacataa taaaaaccat ttcgtctggc aaa
//
LOCUS       DAOOBH010000058        13651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5802_length_13651_cov_5.239114, whole genome shotgun sequence.
ACCESSION   DAOOBH010000058 DAOOBH010000000
VERSION     DAOOBH010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13651
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5802_length_13651_cov_5.239114"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PK218_07120"
     CDS             complement(<1..375)
                     /locus_tag="PK218_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98312.1"
                     /translation="MPTAQTVKSAFFITFNGNCKEALSFYQSCFGGELHIDTFTTPMN
                     GLTETPVITGFLTSNIITIYGSDLVADNGRRIGNFMAIYVHCTNHKERLSYLEKLAPK
                     PQLYVSEKLIEIKNLKVIYETDE"
     gene            complement(400..891)
                     /locus_tag="PK218_07125"
     CDS             complement(400..891)
                     /locus_tag="PK218_07125"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW98313.1"
                     /translation="MENNKMKICLWFESQAEEAANFYSTIFPNTKIGKVLRYSKEAIE
                     IHKMPEGSVMTVEFSINDMDFIGINGGPVFKFNEAISVMVSCDTQEEIDNYWSKLTVD
                     GEELPCGWCKDKYGLVWQIAPTILHQMLSDSNSEKVQRVTHAYMQMKKFDIVNLEKAF
                     AGN"
     gene            complement(918..1127)
                     /locus_tag="PK218_07130"
     CDS             complement(918..1127)
                     /locus_tag="PK218_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98314.1"
                     /translation="MLKKTAGVINSLSVDSIEEVNTMMTNAIKAGATEPRPAMDYGFM
                     FQRSFEDIDGNLWEVFHMDVTKFPG"
     gene            complement(1099..1272)
                     /locus_tag="PK218_07135"
     CDS             complement(1099..1272)
                     /locus_tag="PK218_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98315.1"
                     /translation="MDFYAEIGFTNNPTFTDETAACMVLTEEIYVMLLTHPKFSQFTN
                     KEIIDAKKNCWCN"
     gene            complement(1408..1845)
                     /locus_tag="PK218_07140"
     CDS             complement(1408..1845)
                     /locus_tag="PK218_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW98316.1"
                     /translation="MALINPYLNYNGNAEEAFTFYKSVFGGEFAKVIRFKEMACPENP
                     VAVHEENKIMHIALPIGKNVLMANDVPESMGKVNENENRSKIAIAAESREEADRLFNG
                     LSAGGQIEMPIADSPWGSYFGMFRDKYGIEWMVDFDTNYKGKV"
     gene            complement(1870..2253)
                     /locus_tag="PK218_07145"
     CDS             complement(1870..2253)
                     /locus_tag="PK218_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPW98317.1"
                     /translation="MKKDKIIYWSATIFIVLFEGFMPLATLLFAKQYATAGTQPLGYP
                     DYFAYALIICKVLGCIAISAPNISSKLREWAYAGFTFNLLFATLSHAVVDQNIGFILM
                     PLVILAILAVSYFYNDKIKKQTINQ"
     gene            complement(2275..2697)
                     /locus_tag="PK218_07150"
     CDS             complement(2275..2697)
                     /locus_tag="PK218_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPW98318.1"
                     /translation="MIGREKITVKTTIAAPIAKVWNAFTNPIDIINWNTADASWHCPK
                     SENDLRVGGRFLSRMEAKDGSFGFDFEGTYNIINLHKEITYTMDDGRVAKTLFNEVDN
                     KTEVETTFDAEEENTLDLQRNGWQAILNNFANYVTNKK"
     gene            2827..3807
                     /locus_tag="PK218_07155"
     CDS             2827..3807
                     /locus_tag="PK218_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW98319.1"
                     /translation="MMQIAVFVPEHGVIEAITPPFRSFNTANEFLVAFGKEPKFNVEY
                     VGLNKYVSANNGEYTVKTDRLLKDVKHTDLLITPPTFGDTQRGIDANADAIPLFTKLY
                     KNGSSLASLCIGAFLLAETGLLNGKKCSTHWAYIDEFKAKYPEVEVEDGAIITEYDTI
                     YSSGGASSLWNLILYLIEKYSDRETAVLISKYFALDIGRESQASFAIFRGQRNHDDDE
                     IQKVQDHIEKNYEEKLTIESLAALINSGRRTFERRFKKATNNTAIEYIQRLRIEAAKK
                     FFEASRKNVGEVMFDVGYTDTKAFREVFKKITGLTPIEYRNKFAKTTHEV"
     gene            4195..4437
                     /locus_tag="PK218_07160"
     CDS             4195..4437
                     /locus_tag="PK218_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98320.1"
                     /translation="MIIKYILIFFNTIILLTSCNGQTAKQQIKIDNIVKGDTVKELGS
                     SIMVVYQDSKNTYWFGSWKTGVYKYDGKELINYTTK"
     gene            4675..5226
                     /locus_tag="PK218_07165"
     CDS             4675..5226
                     /locus_tag="PK218_07165"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPW98321.1"
                     /translation="MPKPPNLSTSFGIYSIYKDKKGNLWFGTNPVGVCRYDGKSFKWI
                     TEEDVTEFRNEGANGVRSITEDKNGDFWFNTEYRYGVYDSTTLKSNKFYTRHQSIGGL
                     DGKKNSDLDEYLSIVKDNNNNLWFVTYLDGVWKYDGTKITHYAVKNKSRQIALFCIYK
                     DNKGDLWLGTHGNGAYKFNGITF"
     gene            complement(5400..8003)
                     /gene="clpB"
                     /locus_tag="PK218_07170"
     CDS             complement(5400..8003)
                     /gene="clpB"
                     /locus_tag="PK218_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPW98322.1"
                     /translation="MNIKNLTIKSQEALQLAQQIAQENSNPQLENEHLIKAILEVDEN
                     VTPFLLKKLNVNVDLFKKVIDSTIQSFAKSQGSEIMISRETANTITEANIIAKKMNDE
                     YTSIEHLVMAIFKSKSKVAQILKDQGVTEKGLNSAIEELRKGERVTSASAEENYNALN
                     KYAKNLNELARTGKLDPVIGRDEEIRRVLQILTRRTKNNPMLVGEPGVGKTAIAEGLA
                     HRIVDGDVPENLKDKIVFSLDMGALIAGAKYKGEFEERLKAVVKEVTSAEGDIVLFID
                     EIHTLVGAGGGEGAMDAANILKPALARGELRAIGATTLDEYQKYFEKDKALERRFQKI
                     MIDEPDTESAISILRGIKEKYETHHKVRIKDDAIIAAVELSQRYITNRFLPDKAIDLM
                     DEAASKLRMEINSKPEELDVLDRKIMQLEIEIEAIKREDDETKLKSLGMDLANLKEER
                     NEIFAKWKSEKDVVDNIQMAKQEIEDYKLEAEQAERNGDYGKVAELRYGKIKEAQEKL
                     DIFQTQLAESQEGTSLIKEEVTREDIAEVVAKWTGVPVMKMLQGEREKLLRLENELHH
                     RVVGQEEAIEAVSDAVRRSRAGLQDMKKPIGSFLFLGTTGVGKTELAKALAEYLFDDE
                     NAMTRIDMSEYQERHSVSRLVGAPPGYVGYDEGGQLTEAVRRKPYSVVLLDEIEKAHP
                     DTFNILLQVLDEGRLTDNKGRVADFKNTIIIMTSNMGSHIIQEKFDNLKGSAEAAIEA
                     AKVEVLSLLKQTVRPEFINRIDEIVMFTPLSTENIKEIVGLQLKNVTKMLAQQNITMD
                     ATPEALDYLALKGFDPQYGARPVKRVIQREVLNQLSKEILAGKITTDSIILLDSFDGK
                     LVFRNQAELVE"
     gene            8177..8569
                     /gene="ytxJ"
                     /locus_tag="PK218_07175"
     CDS             8177..8569
                     /gene="ytxJ"
                     /locus_tag="PK218_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HPW98323.1"
                     /translation="MSLFNNLFGKSKVEPMPDVYWNYLESEQQLEEIKKTSFEKPVTI
                     FKHSTRCSISRMSWNQFQKHYNIPNDKMELYYLDLLEHRSLSNAIEEQFQVLHQSPQI
                     LVIKDGVVVFHTSHESIDANDLEEYVSA"
     gene            complement(8559..9743)
                     /locus_tag="PK218_07180"
     CDS             complement(8559..9743)
                     /locus_tag="PK218_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98324.1"
                     /translation="MKKITSLVLFVFVLSACGVRQTRELLTSGEYDAAIENAIDGLRG
                     NKNAKGKQDYIYMLEEAFAKAKERDLRNIETWFKDANPQKLEKIYNTYIALNNRQESI
                     RPLLPLKLLKEGRDAIFPFEDYSDQIVSSKNALSKYLYDNSKALLVTKDKMTIRRAYD
                     DLIYLESINPNFKDTRKLIDEAQFKGTDFVSVYSKNETNMVIPVRLENDLLDFSTYGL
                     NDKWTVYHSNKQKDIKYDYGIVINFRDIKISPEQIKERQFDKEKQIKDGVKTLLDSHG
                     NVVRDSLGNAIKVDNMKTIRISIYEYSQVKACQVTAKVDYIDFANNQLLQSFPLASEF
                     VFTNTYAKYRGDKRACEEAYYPTFDQRPLPFPTNEQMVFDTGNDLKTNLKNIISKNRI
                     RR"
     gene            9912..10127
                     /locus_tag="PK218_07185"
     CDS             9912..10127
                     /locus_tag="PK218_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPW98325.1"
                     /translation="MGLVKIFSGPETLAIDLKLRIENAGVNVVIKNNNQANVFPSLNS
                     SKAVEVFIQENDFMKVNQVVEDFKMGL"
     gene            10133..10939
                     /locus_tag="PK218_07190"
     CDS             10133..10939
                     /locus_tag="PK218_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPW98326.1"
                     /translation="MKALRFIFTSALLLLGVLYSIYLSYVYFNQSEMVFRASKLPNDY
                     QFQFQNNFEEITIPSFDGVKLNGLLFKSKNPKGLIFYLHGNAGSLDSWGNHADIYTRL
                     GYDIFFLDYRGFGKSEGEIDDQEQVYKDITIAYETLMKRYDKNKTIIIGYSIGTGIAA
                     YLASVEQPKMLLLQAPYFNFTEFTTNRVPFYPDVLKKFSFETNNYMVKVKAPVYIFHG
                     KQDNLITYDNALQLQKLFKPKDSLFALESQGHRGINDNYEFQEKLQEILK"
     gene            complement(10960..11523)
                     /locus_tag="PK218_07195"
     CDS             complement(10960..11523)
                     /locus_tag="PK218_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HPW98327.1"
                     /translation="MDNNKELTSEEKEGLLNTLKLRFEKNNHRHEGIDWNNVKEKLET
                     LPKKLWSLNEMERTGGEPDVVAYDAKTKEFVFYDCSAESPSGRRSFCYDRQALEKRKE
                     HKPKNSVMDSAAEMGISLLDEAAYRTLQQLEKVDAKTSSWLKTPDPIRQLGGAIFGDF
                     RYNTIFIYHNGADSYYATRGFRGSLRV"
     gene            11730..12629
                     /locus_tag="PK218_07200"
     CDS             11730..12629
                     /locus_tag="PK218_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPW98328.1"
                     /translation="MNINAITNLITDKLTNWTEILIKLLPNILLAAIVLVVGIFISKY
                     IRKTANKLICKASNNLTLNKLFSSIIYFTAIGIVFFTVLSILKLDKAVTTVLAGAGIL
                     GLGLAFAFQDIAANFISGIFISFRKPLRVGDIVKIGEYMGKIVEVNLRDTVMETFQGQ
                     TVIIPNKEVFQNPIENYSHLKIRRFDLSIGVSYGDDLDIVQEITLNAVKDIDGLSKTH
                     EVKVFFTEFGSSSINLTLNMWINSADQPIYNLVGSEAIKRIKKAYEANDIMIPFPIRT
                     LDFGIKGGTALNEMPVQFLNKAQ"
     gene            complement(12660..13340)
                     /locus_tag="PK218_07205"
     CDS             complement(12660..13340)
                     /locus_tag="PK218_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPW98329.1"
                     /translation="MEYLFAPIQRLYYLKKTIAVLCLLALFSCATKPPFEKGTFKKSD
                     LVELVKLDPSIHLDIRYATNNNFIGRPVYTEARAFLQRPAAEALVKVNTELEPLGYGL
                     LIFDGYRPWSVTKVFWDNTPEDKKNFVADPKKGSRHNRGCAIDLTLYDLTTGKQVAMT
                     SDYDEMNENAYPDFTGGTKEQRNNRDLLRQKMEANGFKVYKYEWWHFDFQGWESYRIE
                     NIPFSEVH"
BASE COUNT         4235 a         2378 c         2263 g         4775 t
ORIGIN      
        1 ttcatctgtt tcatagatta cttttaagtt ttttatttca attaattttt ctgaaacata
       61 aagttgtggt ttaggagcta atttttctaa ataagacaat cgctctttat gattagtaca
      121 atgtacataa atagccataa agttaccaat acgtcttccg ttatctgcta ccaaatcgga
      181 accataaata gtaattatat tagaagttaa aaaacctgtg attaccggtg tttctgttaa
      241 accattcatt ggagtagtga acgtatcaat atgcaattca ccaccaaaac aactttgata
      301 gaaggaaagt gcctctttac aattaccatt aaaagtaatg aaaaaggcac tctttactgt
      361 ttgtgctgtc ggcatgttta aatttttttt atataccgtt taattacctg cgaaagcttt
      421 ttccaaattt actatatcaa attttttcat ttgcatatag gcatgtgtaa ccctttgtac
      481 tttttcagaa ttgctatccg ataacatttg atgtaatatt gttggtgcta tttgccacac
      541 caaaccatat ttgtctttgc accatccaca aggtagttct tctccatcaa cagtcaattt
      601 actccaataa ttgtctattt cttcttgagt gtcacaactc accattacgg atatggcttc
      661 attaaattta aaaacaggac caccattaat gccaataaaa tccatatcgt ttatactaaa
      721 ttcaactgtc attactgatc cttcgggcat tttatgtatt tctattgcct ctttactata
      781 gcgtaatact tttccaattt tggtattagg aaagatagtg ctataaaaat ttgctgcttc
      841 ttctgcttgg ctttcaaacc acaaacagat tttcattttg ttattttcca tttttattgt
      901 tttttttaga gtgttgatta tccgggaaac ttagtaacat ccatgtggaa tacttcccaa
      961 agattaccgt caatatcctc aaaacttcgt tgaaacataa atccataatc cattgctggt
     1021 cttggttctg ttgctccagc ttttatcgca ttggtcatca ttgtatttac ctcttctata
     1081 ctgtcaacag ataaagaatt aattacacca gcagtttttt ttagcatcaa taatttcttt
     1141 gtttgtaaat tgtgaaaact taggatgtgt tagtaacatt acatagattt cttctgtgag
     1201 caccatgcaa gcagcggttt cgtcagtaaa agttggattg ttagtaaacc caatttccgc
     1261 gtaaaagtcc attgattttt gtaagtctga aatgggcaaa ttgataaaga ttttctttga
     1321 cattatataa cttttaaagt tattattttg ataaaaatag gagcgtgtca ctattcctaa
     1381 attacgctgt tgatattata gaagacttta tacttttcct ttgtaatttg tatcaaaatc
     1441 aaccatccac tcgatgccat atttgtctct aaacattccg aagtaagaac cccaaggact
     1501 atcggcaata ggcatttcga tttgtccgcc agcggatagt ccgttgaata aacggtctgc
     1561 ttcttcacga ctttcggctg caatagctat tttagatcta ttttcgtttt catttacttt
     1621 tcccatactt tcgggaacat catttgccat caaaacattc ttgccaatag gcaacgcaat
     1681 gtgcattatt ttattttctt catgcactgc tactggattt tctggacatg ccatttcttt
     1741 gaaacggata acttttgcaa attctccgcc aaatactgat ttgtaaaatg taaatgcttc
     1801 ttctgcattt ccattatagt ttaaataggg attgattagt gccataattt tttttatata
     1861 tagtttttat tactgattta tggtttgttt tttaatttta tcgttataaa aataagagac
     1921 tgccagtatt gctaatatta ctaacggcat tagaataaat ccaatgtttt ggtcaactac
     1981 ggcatgactt agtgtggcaa ataacaaatt gaatgtaaac ccagcataag cccattccct
     2041 tagtttagaa gagatatttg gtgccgatat agcaatgcat cccagcactt tacagattat
     2101 taaagcataa gcaaaataat cgggataccc caaaggttgt gttcctgcag tggcatattg
     2161 ttttgcaaat aggagcgtgg ctagtggcat gaagccttca aaaagcacta taaatattgt
     2221 agcactccaa taaatgattt tgtctttttt catattgatt tatcttaagg ttgtttactt
     2281 tttattcgtt acatagtttg caaaattgtt gagaatcgct tgccagccat ttctttgtaa
     2341 gtcgagagtg ttttcttctt ctgcatcaaa ggttgtttct acttctgttt tgttatcaac
     2401 ttcgttaaac aatgtttttg ctactctgcc atcatccata gtataagtga tttctttgtg
     2461 gaggttaatg atgttataag tgccttcaaa gtcaaagcca aaactaccat ctttggcttc
     2521 cattcgggat aaaaatcgtc ctccaaccct aaggtcattt tcactttttg gacaatgcca
     2581 actggcatcg gcagtattcc aattgataat gtcaatagga ttagtaaagg cattccaaac
     2641 tttagctatt ggtgccgcta tcgttgtttt tacggttatt ttttctcttc caatcatttt
     2701 tttttagttt ttattgttat acaaagatgg caactatttt agtatattaa aatgtgtgtt
     2761 taagtcaacc ttaggggtag attatgacaa ttagatttta tatatttgac attaaaatta
     2821 ttaaaaatga tgcaaatagc tgtttttgtt cccgagcatg gtgttattga agccattaca
     2881 ccacctttta gatcattcaa tactgccaat gaatttctgg ttgcttttgg aaaagaacct
     2941 aaatttaatg ttgagtatgt agggttaaac aagtatgtat ctgccaataa tggcgagtac
     3001 accgttaaga ctgaccgatt gctaaaggat gtaaaacaca ctgacctgtt aattactcct
     3061 ccaacttttg gtgataccca aagaggtatt gatgccaatg ccgatgccat acctcttttt
     3121 actaaattgt ataaaaacgg aagtagttta gcaagtcttt gcatcggtgc ttttttgttg
     3181 gctgaaacgg gtttattgaa cggcaaaaaa tgttctactc attgggctta tattgacgaa
     3241 tttaaagcca aatatcctga agtagaagta gaagacggtg ccattatcac cgaatatgat
     3301 actatatata gtagcggtgg tgctagcagc ttgtggaact tgattttata tctgatagaa
     3361 aaatattcgg atagagaaac agcggtattg atttcgaaat actttgcctt agacattggt
     3421 agagaaagcc aagcttcgtt tgccattttt agaggacagc ggaatcatga cgacgatgaa
     3481 attcaaaaag tacaagacca tattgaaaag aattatgaag aaaaactaac tatcgaaagt
     3541 ttagctgcat taataaattc tggaagaaga acttttgaaa gacgatttaa aaaagcaaca
     3601 aacaacacag ctattgaata cattcagcgt ttgcgcattg aagctgccaa gaaattcttt
     3661 gaagcttcga gaaaaaatgt tggcgaagta atgtttgatg ttggatacac cgatactaag
     3721 gcttttcgag aagtatttaa aaaaataacg ggattaacac cgatagaata tagaaataaa
     3781 tttgccaaaa caactcatga agtgtaaaca gagaagctat tataagccaa attttgtttc
     3841 tttgacgaag gaaagttagc ttaagagttt tactctgttg tgttattggt tgtagttctt
     3901 gttgttgctc cagagtaact gtatggctct ttacaatgtg ttgttgagat tggtaattag
     3961 ttattggttt ttttttatat ttgagaaacg aaacaaaata acgacaatct acccgatttt
     4021 ggtgcatttg cgaaagtttg tttcgaatgt atataagttt agtgaaatgt ccgaaagcct
     4081 atcactcaat aggcagacac tttgctgaac ttgaactttg aagaaatcaa acgacttcgt
     4141 caagtttccg gctattaatt gtaactcaaa aacgaccata caaatttcaa tctgatgata
     4201 ataaaataca tattaatatt tttcaataca ataattttat tgacatcttg taatggacaa
     4261 accgctaaac aacaaattaa aatagacaac attgtaaaag gcgacacggt aaaagaactt
     4321 ggtagcagta taatggttgt ctatcaagac agtaagaata cttattggtt tggtagttgg
     4381 aaaacaggag tttataaata cgatggaaaa gaattgataa attacacaac aaagtaagga
     4441 ctgccaaaca atagaattga tgaaataaaa gaagatgagt ttggcaacat atacttcact
     4501 agttgtcacc caacttctac gatagttaaa tttgacggaa atacttttac tacattatcc
     4561 gcaatcccca gtaacgaatg gaagcttaaa tcaaatgatt tatggtttag acattcttat
     4621 gaatttgaga ataaagtata tcggtatgat ggtattactt tatatgaatt gcagttgccc
     4681 aagccaccaa acctctctac ttcttttgga atctatagca tttataaaga taagaaagga
     4741 aatctatggt ttggcacaaa tcctgttgga gtttgtcggt acgatggaaa gtcatttaaa
     4801 tggattacag aagaagatgt gacagaattt cgcaatgaag gtgcaaacgg agtccgttca
     4861 attacggaag acaaaaatgg cgacttttgg tttaataccg aatatcgata tggtgtttat
     4921 gatagtacaa cattaaaaag caataaattt tatacaagac atcaaagcat aggtggttta
     4981 gatgggaaaa agaatagcga tttggacgag tatctatcaa tagtaaaaga taacaataac
     5041 aatttatggt ttgtaaccta tcttgatggt gtctggaaat atgatggaac aaaaattact
     5101 cattatgcag ttaaaaacaa atccagacag attgctttat tctgtatata caaagacaac
     5161 aaaggcgatc tttggcttgg gactcacggc aacggagcat ataaattcaa tggaataaca
     5221 ttttaaaaat tcataaaata aaaaataaaa aataaaaaaa agactaaaga ttaataccct
     5281 aaatatttta aaaagaaacc tttaccatta gcgtctaatc cacatcacaa aagtaaaaaa
     5341 aacctaacaa gcttttacac tcattaggtt ccaaccaaaa aacaatcatc aatcctttat
     5401 tattctacta attctgcttg gtttctaaac actagcttgc cgtcaaaact atcgagcaag
     5461 atgatgctat cagtagtaat ctttccagct aatatttctt tactcaattg gttcaatact
     5521 tcgcgttgga taaccctttt tacaggtcgc gcaccatatt gtggatcaaa gcctttcagt
     5581 gctagatagt ctagtgcttc gggtgtagca tccatggtaa tgttttgttg ggctaacatt
     5641 ttggttacat tttttagttg taaaccaaca atttctttta tattttcggt gctcaacggc
     5701 gtaaacatca ctatttcatc aattctgttg ataaactcag gacgtaccgt ttgtttaagc
     5761 aagctcaata cttctacttt tgctgcttca atagcggctt ctgccgaacc ttttagattg
     5821 tcaaacttct cttgaataat atgacttccc atattactgg tcatgattat aatcgtattt
     5881 ttaaaatccg ccacacgacc tttattgtcc gtcaatcgtc cttcatcaag cacttgcaat
     5941 aggatgttaa acgtatcggg atgtgctttt tcaatttcat ctaaaagtac tacgctatat
     6001 ggttttcttc gcacggcttc ggtcaattgt ccaccttcgt catagccaac atatcctgga
     6061 ggagcaccta ctaatcggct cacactgtgc cgttcttggt attcgctcat gtcaatacgt
     6121 gtcatagcgt tttcatcatc aaataggtat tctgccaagg cttttgccag ctcggttttt
     6181 ccaacaccag tggttcctaa aaacaagaac gaaccaatgg gtttcttcat gtcttgcaat
     6241 ccggcacggc tacggcgaac agcatcacta accgcttcaa tagcttcttc ttgaccaacc
     6301 acacggtggt gcaattcgtt ttccaaacgc aacaactttt cacgttcgcc ttgcagcatt
     6361 ttcatcacag gaacgcctgt ccatttggct accacttctg caatgtcttc tcgggtcact
     6421 tcttctttaa tcagcgaagt accttcttga ctttcggcca attgggtttg aaaaatgtct
     6481 agtttttctt gtgcttcttt tatctttcca taacgcaatt cggctacttt tccgtagtca
     6541 ccgtttcgtt cggcttgttc ggcttcaagt ttataatctt caatttcttg ttttgccatt
     6601 tgaatgttat ctaccacatc tttctccgat ttccatttgg caaatatctc gttgcgttct
     6661 tcttttaaat tcgccaagtc cattcccaaa ctttttaatt tggtttcatc atcttcgcgt
     6721 tttatggctt caatttcaat ttcaagttgc atgatttttc tatccaaaac atccaattct
     6781 tctggtttgg agttgatttc catgcgtaac ttagaagccg cttcgtccat taagtctatg
     6841 gctttatcag gaagaaaacg attggtaata taacgttgtg ataattctac tgcagcaata
     6901 atggcatcgt ctttaatacg cactttgtgg tgggtttcat atttctcctt aataccacgc
     6961 aaaatcgaaa tcgcactttc ggtatctggt tcgtcaatca ttattttctg aaaacgtctt
     7021 tcgagtgctt tgtctttttc aaaatacttt tgatattcgt ctaatgtagt agcaccaata
     7081 gcacgcagtt cgccacgagc caaggcaggt ttcaaaatat tagcagcatc catggcacct
     7141 tcgccaccgc ctgcaccaac taaagtgtgt atttcatcaa taaagagaac aatatcacct
     7201 tcggcagaag ttacttcttt cactacagct ttcaagcgtt cttcaaattc acctttatat
     7261 ttagctccag caatcaatgc acccatatcc agtgaaaaaa caatcttatc ttttagattt
     7321 tcaggaacat cgccatcaac aattcggtgt gccaaccctt cggcaatggc tgttttacca
     7381 acaccaggtt cacccacaag cattgggttg tttttggttc ttcgggttaa gatttgcaac
     7441 acgcgacgaa tttcttcatc tcgaccaatc acaggatcca attttccggt acgtgccaat
     7501 tcgttgaggt ttttggcata tttgtttagt gcattataat tttcttctgc cgaagccgaa
     7561 gtaactcttt cgccttttcg caattcttcg atagcactgt tcaatccttt ttcggtaaca
     7621 ccttggtctt ttaaaatctg tgctactttg cttttcgatt tgaatattgc cattactaaa
     7681 tgctcaatcg atgtgtattc gtcgttcatt ttcttggcga taatgttggc ttctgtaatg
     7741 gtatttgccg tttcacgcga aatcataatt tcgctacctt gcgatttggc aaagctttga
     7801 attgtgctat caattacttt tttgaaaagg tcaacgttta catttaattt ctttaaaaga
     7861 aatggcgtaa cgttttcatc tacttcgagt atggctttta tcaaatgctc attctccagc
     7921 tgtggattgc tgttttcttg cgcaatttgc tgtgctagtt gcaacgcttc ttgcgatttg
     7981 atggttaaat tttttatatt catattcttt atatttttta aaagaaacgt tagtataaat
     8041 cgtttctata tttttttttt aaaacttcta tttatattag tcaatatgtg ttccataatt
     8101 aaaatcaagt caaaatggct gatatttcaa gataatttac tatttttcaa gtcaaaatgt
     8161 cttaaaaccc gtcaatatga gtttgtttaa taatcttttt gggaaaagta aagtagagcc
     8221 aatgccagat gtgtattgga attatcttga aagcgaacag caattggaag aaataaaaaa
     8281 aacttctttt gaaaaacctg tgactatctt taaacacagc acgcgatgca gcatcagtag
     8341 aatgtcttgg aatcaatttc agaagcacta caacattcct aatgataaaa tggaattata
     8401 ttatctagat ttattagaac accgttcgct atcaaatgcc attgaggagc agtttcaagt
     8461 gctacatcaa tcgcctcaaa ttttagtaat taaggatgga gtagtggttt ttcatacttc
     8521 gcatgaaagc attgatgcca atgatttgga agaatacgtt agcgcctaat tctattttta
     8581 gaaataatat tttttaagtt cgtttttaaa tcattacctg tatcaaaaac catttgttcg
     8641 ttggttggaa acggtaatgg tctttggtca aaagtagggt agtacgcttc ttcacatgct
     8701 cgtttatcac cacgatattt ggcataggta ttagtaaaaa caaactcgct tgctaacggg
     8761 aatgattgta acaactgatt gttagcaaaa tcgatataat ctactttagc ggtaacttga
     8821 cacgctttta cttgtgaata ctcgtagata ctgatgcgaa tggttttcat attatctact
     8881 tttattgcat tacctaaact gtctctaaca acatttccat gcgaatctaa aagggtttta
     8941 acaccgtctt taatttgttt ctctttatca aattggcgtt ctttaatttg ctcaggcgat
     9001 atttttatat ctctgaaatt gatgactata ccataatcat atttaatatc tttttgttta
     9061 ttgctatgat aaaccgtcca tttatcattc aaaccataag tgctaaagtc taacaaatca
     9121 ttttccaaac gaacaggaat gaccatgttg gtttcgttct tagaatacac gctaacaaag
     9181 tcggtacctt taaattgtgc ttcgtctatt agttttcggg tatctttaaa gttaggattg
     9241 atgctttcta aatatatcaa atcatcatag gcacgccgaa tggtcatctt gtctttggta
     9301 acgagcaatg cttttgaatt gtcgtataga tatttagaca aagcattctt gctgctcact
     9361 atttggtctg aataatcctc gaaaggaaaa atagcatctc taccttcttt caataacttc
     9421 aagggcaata aaggtcgtat gctttcttga cggttgttta gtgctatata agtgttgtaa
     9481 atcttttcta acttttgtgg attggcatct ttaaaccaag tttcaatatt tcttaaatct
     9541 cgctcttttg cttttgcaaa agcttcttct agcatataaa tataatcttg ttttcctttg
     9601 gcatttttgt ttcctctcaa accatcaatc gcattttcaa tggcagcatc atattcgcca
     9661 gaagttaaca attctcgtgt ttgacgaact ccacaagcgg aaagcacaaa cacaaataac
     9721 acaagagaag taattttttt catagactaa aagtttaagc aaatatataa atacttgtta
     9781 atatcatgga tgtttcttaa gaatttaatt aaatttgtgt actgtaaaat agttcattat
     9841 agtacattct ataaagttga agcagcctac tttatttttc aatacattta atacttaaaa
     9901 caaataatat catgggttta gttaaaattt tttcgggacc agaaacctta gccatcgatt
     9961 taaaattgcg catagaaaat gctggagtaa acgttgtgat aaaaaataac aaccaagcaa
    10021 acgtatttcc tagtctaaac agctctaaag ccgtagaagt tttcattcaa gaaaacgatt
    10081 ttatgaaagt caaccaagta gtggaagatt ttaaaatggg attgtaggcc gcatgaaagc
    10141 attgcgattt atatttactt ctgcactatt actattaggt gttttataca gcatttattt
    10201 aagctatgta tatttcaatc aatctgaaat ggtttttaga gccagcaaac ttccaaatga
    10261 ttaccaattt caatttcaaa ataacttcga ggaaattacc attccgtctt ttgatggtgt
    10321 taagttaaat ggattattgt ttaaatccaa aaacccaaaa ggattaattt tttatcttca
    10381 tggcaatgct ggttcgctag acagctgggg gaatcatgcc gatatataca ctcgtttggg
    10441 ctacgatatt ttctttttag attacagagg atttggtaaa agtgaaggcg aaattgacga
    10501 tcaagaacag gtatataaag acattaccat cgcttatgaa acgttgatga aacggtatga
    10561 taaaaacaaa acgattataa ttggttattc aattggaact ggaatcgctg cttatttagc
    10621 atcagtcgaa caaccaaaga tgttgttgct tcaagcaccc tattttaatt ttacagaatt
    10681 tactactaat agagttcctt tttatccaga tgtattaaag aaatttagtt ttgagaccaa
    10741 taactatatg gtgaaagtaa aagcacctgt ttatattttt catggcaagc aagacaattt
    10801 gattacatac gataatgcac tccaacttca aaagcttttt aaaccaaaag acagtttgtt
    10861 tgctctagaa agccaaggac atcgtggtat taatgataat tatgagtttc aagaaaagct
    10921 tcaagagatt ttgaaataaa gtgcatatac tattagcttt tacactctga gcgaacctct
    10981 aaaacctcta gtggcgtagt acgaatctgc accattatgg tatataaaaa tggtgttgta
    11041 tctaaaatcg ccaaagatgg caccgcctaa ttgtctaata ggatcgggtg ttttgagcca
    11101 gcttgatgtt ttggcatcta ctttttctag ttgttgtaag gttcggtaag ctgcttcatc
    11161 caaaagtgaa attcccattt ctgccgcact atccattaca gagtttttgg gtttgtgttc
    11221 ttttcgtttt tctaatgctt gacggtcata acaaaaactt ctgcgaccac ttgggctttc
    11281 tgccgaacag tcataaaaaa caaactcttt tgttttagca tcataggcta caacatcggg
    11341 ttctccgcca gtgcgctcca tttcgttcag cgaccatagt ttttttggca aggtttctag
    11401 tttttctttt acattgttcc aatcaatgcc ttcatggcgg tgattatttt tctcaaaacg
    11461 aagcttcaaa gtgtttagca gtccttcttt ttcttctgat gttaattctt tgttgttgtc
    11521 cataagatag tttttgttgt agttgtgtaa atttaacgca aaagtataaa tacacaaaac
    11581 tgctttaaat tttgtttttt aaaaactaaa aataagtgat ttctttaatt ttattgatta
    11641 aattgaagta ttatccgggt tgtataattt tgttttccat cattaaaagt agtatattag
    11701 caataattca aaaaaaacta tttaaaatta tgaacattaa tgctataaca aatctaataa
    11761 ctgataaatt aacaaattgg acagaaatat taattaaatt gctacctaat attttattgg
    11821 ctgcaatagt cttagtagtt ggaattttta tatcaaaata cattcgtaag actgctaata
    11881 aactaatatg taaagcttca aataatttaa ctttaaacaa actttttagt tcgataattt
    11941 attttactgc aattggcatt gtatttttta ctgttttaag catattaaaa ttagacaaag
    12001 cagttactac ggtattagca ggtgctggta tccttggatt aggattggct tttgcttttc
    12061 aagatatagc tgccaatttt atttcgggca tatttatttc atttcgaaag cctttacgag
    12121 taggtgatat tgttaagatt ggtgagtata tgggcaaaat tgttgaagtt aatttgcgag
    12181 acactgtgat ggagacattt caaggtcaaa cggttataat tccaaataaa gaagtgtttc
    12241 aaaacccaat agaaaattat tcacatctca aaatacgacg ttttgattta agcattggtg
    12301 tttcctatgg cgatgatttg gatatagttc aagaaataac attgaatgct gttaaagata
    12361 tagatggtct atctaaaaca cacgaagtga aagtgttttt tactgagttt ggcagcagtt
    12421 ctatcaattt aaccctaaac atgtggatta attctgccga tcaaccgatt tataacttag
    12481 ttggaagtga agctatcaaa cgcatcaaga aagcttatga agctaacgat attatgatac
    12541 cattccctat aagaacatta gattttggca ttaaaggagg aacagcttta aacgaaatgc
    12601 ctgttcaatt tttaaataaa gctcagtaat agaacaggtt agtatttctt tactaaaaat
    12661 caatgcactt cagaaaatgg aatgttctca attctataag attcccaacc ttgaaaatca
    12721 aaatgccacc attcatattt atagacttta aaaccattgg cttccatttt ttgtcttaac
    12781 aaatctctgt tgttgcgttg ttctttagtt ccgccagtaa aatcgggata agcgttttca
    12841 ttcatttcgt cataatcgct ggtcatggct acttgttttc ctgttgttag gtcatataag
    12901 gtcaaatcta tagcgcaacc tctgttgtgt cttgaacctt ttttggggtc ggctacgaag
    12961 ttctttttat cttcaggcgt gttatcccaa aacactttgg ttacactcca cggacgatat
    13021 ccatcaaaaa ttaataaacc atagcccaaa ggttctagct cagtattaac ttttactaat
    13081 gcttcggcag ctgggcgttg caaaaatgct ctggcttcag tatatactgg tcgtccaata
    13141 aagttgttat tcgttgcata acgaatgtct aaatgaatac ttgggtcgag ttttaccaat
    13201 tctactaaat ctgatttttt gaaagttcct ttttcaaatg gtggtttggt ggcgcaacta
    13261 aagagtgcta ataaacaaag tactgcaatc gtttttttta aataataaag tctttggata
    13321 ggagcaaaga gatattccat tttgtttttt atttgcaatt actatggctt gattatcatt
    13381 aatttaaaac ctatttattt cacaattata aagtacattt tgatgaattt agagtcttgt
    13441 tctaagctac atccactctc catttttcaa aaataactaa aaaagaatag tttataaaat
    13501 ttgtattgac acaaaacaga cacaagttag aaataaaaaa tccccaaacc ttaactattt
    13561 attaaagatt ggggattaaa ttgtgacctc gactggattc aaaccagtaa cctcttgagc
    13621 cgtaatcaag tgcgctattc agttgcgcca c
//
LOCUS       DAOOBH010000059        13583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5839_length_13583_cov_4.149616, whole genome shotgun sequence.
ACCESSION   DAOOBH010000059 DAOOBH010000000
VERSION     DAOOBH010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5839_length_13583_cov_4.149616"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PK218_07210"
     CDS             <1..678
                     /locus_tag="PK218_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPW98330.1"
                     /translation="GTMPTQPTDLACYETATFNTTTCVWDVTGTMPVAQEVVAESCNE
                     AQFDTIDLASLLPTGTPQGGTWTNVDNVAGLNGTIFNGFEIPVGTYTFSYEYDVNQPC
                     LAKINVLVNVNTNCGVLPCENIIIHNAFTPNGDSLNQFFSIEHIEDFDCYPTNKVEIY
                     NRWGVLVYETTNYDNVTRRFEGISEGRVTVSKSSELPTGTYFYIIQYTTTEGNTVSKN
                     GYLYLTR"
     gene            736..1647
                     /locus_tag="PK218_07215"
     CDS             736..1647
                     /locus_tag="PK218_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPW98331.1"
                     /translation="MKTKILIFALMLTCYCSFAQQDSQFTQYMYNTINVNPAYAGSRG
                     VMSIFGLHRTQWVGLEGAPTTNAFSINTPINDSNLGLGISFLNDKIGPTNNNTITADL
                     SYTIQTSETYKLSFGIKASGNIFNLDVNKLNPQDADDPNLQNYNSKFSPNFGAGIYLH
                     SDKLYFGVSVPNFLQDNKYNDNSVTVFKEKMTFYTIGGYVFDLSPDVKFKPAFLTKVV
                     TGAPLQVDLSGNFLLFDKFMLGAAYRWDAAISGMAGFQVTDGLFIGYGYDRETTNLRN
                     YNSGSHEIFIRFELFNRVKKLVSPRFF"
     gene            1660..3600
                     /locus_tag="PK218_07220"
     CDS             1660..3600
                     /locus_tag="PK218_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW98332.1"
                     /translation="MKNKIIYTALLLFIAFNSFSQTVGKEKKGNKDYEQYAYIDAIKT
                     YERLYEKGYKSPDMLLKLGNSYYFKADLEKAAKYYDELYTTNPDQEAEYYYRYSQSLR
                     GIKEYKKADEMMLKFNAKSGNDSRAALFNQNKDYLAEIKRNSGRFKLENAGINSKYSD
                     YGTAFVDDKVVFCSARDTGNFSKRIHTWTGEYFTNLYSAKLAEDGSLSDVQKFGNKLN
                     SKYHEDTPVFSKDGKTVYFTRNNYLDKRGYDDGKVTLLKIYKANLDEKGKWENITPLP
                     FNSDSYQTAHPTLSPDGKMMYFVSDMPGSLGKSDLYKVAISEDGTFGTPINLGKTINT
                     EGRETYPFVTAKNELYFSSDGHPGLGGLDIFAAKIPEDGNFTNIQNIGEEANSPKDDF
                     AFIINTDTKRGFLSSNRDGGEGNDDIYKFLETRELFIEQAIFGVVTDAKTNEVLPNTK
                     ITLLDEKFNKIAETTTDEKGYYEFLKLEPNTKYYLRTEKPEYDVKEIPVITGKEDGRT
                     EVNIELDKTAQPIPVGGDIADVFKINLIYFDLDKWNIRPDAAIDLAKVLDVLEQYPTM
                     KIDIRSHTDSRASHAYNEKLSDRRAKSTMAWLVSKGISKDRLTAKGYGETQLVNKCAD
                     GVQCSEAEHQLNRRSQFIIAEL"
     gene            3669..4832
                     /gene="nhaA"
                     /locus_tag="PK218_07225"
     CDS             3669..4832
                     /gene="nhaA"
                     /locus_tag="PK218_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HPW98333.1"
                     /translation="MKITKLFKDFFDSEKVGGFLLIICTIISLLFANSFFSNEYLNVW
                     HHPFAGKPVEYWINDGLMAIFFLLIGLELEREIYIGELSNFKKASLPIFAAIGGMLVP
                     AGLYLFLNYGTDTQSGAGIPMATDIAFALGVLSLLGNKVPTSLKVFLTALAVIDDLGA
                     ILVIAIFYTKNLDFGNLSIALGIFAFLLVLNRLKVRNLIPYLVLGVFMWYFMLNSGVH
                     ATITGVLLAFAIPFGNGSKKSTSYLLQNALHKPVTWIILPLFAMANTAIIIDSDWHYA
                     LTHKYTIGIALGLIVGKPIGISLFSYFSVKMKLSSLPEDLNWKSIIGVSFLGGIGFTM
                     SIFITLLAFNDIDHISHSKIMILLSSITVALIGLLFLKSILKKDYIDEVSNDN"
     gene            complement(4843..5913)
                     /gene="corA"
                     /locus_tag="PK218_07230"
     CDS             complement(4843..5913)
                     /gene="corA"
                     /locus_tag="PK218_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPW98334.1"
                     /translation="MRKIKYKKGRKVQTKLLEYTGIHQETLAELQLFVYDSNDLTEFN
                     DFKVSQLEKCIDVSKNNWLNLHGLNDESIIESIGDFLKVDNFMLSDVVNTTKRSKLEE
                     YQDVIFFNIKSILPVENSDNIQVEQISFLLKKEILVSFQEKRSDFFMHVRERIRTHSG
                     LVRDKKSDYLLYLLLDAVMENFYITLENEENRVEALINLTKTNTQLQILEKIEKHRDN
                     FNFLKRSIIPLRDSLFSIKSMREDNVFNVIEDDTFSFFSRLHQKSIELLEQIEYDLLS
                     LESATNFYFSTQNQKLNEVMKTLTVVSVFFMPLTFVVGVYGMNFDNMPELHWKYGYFV
                     ILACMFVLLLGMIYYFKKKKWY"
     gene            5982..7217
                     /gene="hutI"
                     /locus_tag="PK218_07235"
     CDS             5982..7217
                     /gene="hutI"
                     /locus_tag="PK218_07235"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPW98335.1"
                     /translation="MQTLIINIKELLQVRETHIDKVSGAEMAILPSIKNAFLLIENDS
                     IIDFGEMNNCTNFDNKKIIDAKGKIVLPTWCDSHTHLVYAGNREQEFVDRINGLSYEE
                     IANRGGGILNSSKKLNETSEEEIYNQSKLRLEEIMQQGTGAVEIKSGYGLTVDGELKM
                     LRVIKKLAQNYPITIKATFLGAHAFPLEYRENHAAYIDLIINEMLPQIAEEKLADYID
                     AFLETGYFSVEETERIMLAGKKYGLLAKIHVNQFTAINGIEACVKHNALSVDHLEIVT
                     DEDIKVLKNSETMPVALPSCSYFISIPYTPARRLISAGLPLALATDFNPGTTPSGNMN
                     FVVATACIKMKMTPEEAINAATINGAYAMGISQTHGSITKGKKANFIITKPLNSFYEI
                     PYLFTSNLIEKVFINGNCI"
     gene            complement(7268..8152)
                     /locus_tag="PK218_07240"
     CDS             complement(7268..8152)
                     /locus_tag="PK218_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98336.1"
                     /translation="MENQNSNSKLKSIVAILAVLLVGSLIYIFKLTSDAKTMQNIVTV
                     TKSEKEDVLKDLAELKTSYDAAISENTSISDELIAERDKVEKLIQELKTSKGDNASLR
                     KYRDQYKALEQKMKNLMAEVEVLKTQNQQLTTNLDSTKVVLEDSKIYNQVLVGQNEEL
                     AKTVEKGQKLSVTNLKTAAYKVRSSGKQISTDKASRADMLEVKFTIAENKIAKSGDKV
                     YYVQVIDSKNNVLGDKATISFGDTSLTYSFTTNVAYQNKTVEVSEQLPGKDFAKGTYF
                     VNVFDKDELVSKSSFTLR"
     gene            8248..9303
                     /locus_tag="PK218_07245"
     CDS             8248..9303
                     /locus_tag="PK218_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HPW98337.1"
                     /translation="MEKLIPFSLNDLAKATNHRSGELKFGEKMQTIPKDISPLEYLKN
                     CDAKFVLIGVPEDVGVRANYGRPGAATAWESAIKGIANIQHNRFCKGSQILVLGTLDV
                     SEQMEQAKELKFNDTHDRKKLNELVTQIDKDLAHIISCIVKNNKIPIIIGGGHNNAYG
                     NIKGTALGLGKPINVINFDAHSDFRILEGRHSGNGFSYAFEEGFLKKYFIFGLHENYT
                     SKNVLDNLKKLEDRINYNTYDEIGIRKEKVFEEELNKALNFIKSEPFGIEIDLDALPN
                     IPSSAMTLSGFSVEEVRNFINHFGKHKNSKYIHICEGAPDLGDEKNSHLIGKLIGYFV
                     TDFVKSKREATTTNGKN"
     gene            9349..10602
                     /locus_tag="PK218_07250"
     CDS             9349..10602
                     /locus_tag="PK218_07250"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW98338.1"
                     /translation="MLFEDLSLSKSIQRAVFEEGYTQPTPIQEKAIPHILAGKDLVGC
                     AQTGTGKTAAFAIPIIHNLHRMVGSSKKTKVIRCLVVTPTRELAVQIGESFDTYGKYT
                     NLRQLTIFGGVSQVPQVDQLKKGIDILIATPGRLLDLHKQGFIDFDHLHYLVLDESDL
                     MLDMGFINDVKKIVKLVPTNRQTLLFSATMPLAIRELSETFLNKPEYVSVTPVSSTAE
                     IIEQQIYFVDKENKRGLLYHLIRNENLKNVLVFVRTKHGADNVVKALKKHGVNAEAIH
                     GNKSQNARQRVLDEFKNKDISVLVATDIAARGIDIESLPFVINFDLPNIPETYVHRIG
                     RTGRAGNDGISISFCGRDEEPYWKDILKLIKVNVKTMKDHPFPWKEGTPNPETKPDLR
                     NKNKNKTANTSRKSEASKKNKKRWY"
     gene            complement(10599..12815)
                     /locus_tag="PK218_07255"
     CDS             complement(10599..12815)
                     /locus_tag="PK218_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW98339.1"
                     /translation="MIYQNRYRILFLLLFVNVLSLAQEVALTKKEQEQTIKTALDQAV
                     HHFENTHFDKALRASKAALIKSFEINSNSYIAQSYNTIGVIYDECNDSKRAIEFYEKA
                     LRFARQINNDTLNNWIYNNLGSAYYFNNIDTDKGISYYKKALYFAKKLKDENQIIHIN
                     LNLASAYLATNEDQKADSILKTIKEAVFKNNTDEIKLSYYVLMGIKNTHDNKKENAEE
                     FYFKAKDLAEKNHFSSNLLNVYENLAKHFGYFNEQTKQEHYDAKHDSLYNVLYSDEKM
                     DSIEKQALDIELDEYKIQLEKVELQSEKNSKIVETSKLVLVLFMLIVITLLLLLLTLY
                     KSIKTREKLNLELKNTNEALSIAKEKAEEASQMKTQFVSTITHELRTPLYGVIGITNI
                     LLDEHKELANSPHLNSLKFSAKYLLSLVNDILQISKIEERKVVLENLIFNLTDEIDGI
                     RNSIEYIAERNNNKLIIEIDTAIPEFLIGDKLRLSQIIMNLVSNSLKFTKNGEVIITA
                     DLERVEDTMYYITFKVIDNGVGIAKEHQQVIFDKFVQIERKEEDYQGTGLGLSIVTSL
                     IKLFKSEIHLESEENVGTSFTFTIGFIHDEKQCVALINEIEVDLSSHHTYTILVIEDN
                     KINQLVTKKIIQNSNLTCTIVDDGYAALVALEREHFDLILMDINMPLINGFDTTRKIR
                     EKGYTMPIIALTAFDKQEVSEEVYASGMNDILIKPFEPTKLYQIISTHIPKKESDD"
     gene            complement(12893..>13583)
                     /locus_tag="PK218_07260"
     CDS             complement(12893..>13583)
                     /locus_tag="PK218_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW98340.1"
                     /translation="KNVAKAFGDKLLYDNLNFTLPQAGIVGIIGPNGAGKSTIFRMIM
                     GEQQTDSGEFNVGETVKIAYVDQAHSNIDVNKSIWENFCDGQELILMGGRQVNSRAYL
                     SRFNFGGSDQNKKVSALSGGERNRLHLAMTLKEEGNVLLLDEPTNDLDINTLRALEEG
                     LENFAGCAVVISHDRWFLDRICTHILAFEGDSEVYFFEGSFSEYEENKRKRLGGDLTP
                     KRIKYRKLIRN"
BASE COUNT         4633 a         2210 c         2149 g         4591 t
ORIGIN      
        1 ggtactatgc caacacaacc tactgactta gcgtgttacg aaacagctac gttcaataca
       61 acaacttgtg tttgggatgt aacgggtact atgcctgttg ctcaagaagt agttgcagaa
      121 agttgtaatg aggctcaatt tgacacaatc gatttagctt ctttattacc tactggaacc
      181 ccacaaggtg gaacttggac taatgttgac aatgttgctg gtttaaatgg aactattttt
      241 aatggatttg aaatacctgt aggaacttac actttcagct atgaatatga tgtaaatcaa
      301 ccatgtctag caaaaattaa tgttttagta aatgttaata ctaattgtgg tgttttaccg
      361 tgtgaaaata taatcattca caatgcattt acaccaaatg gtgattcatt aaaccaattc
      421 tttagtatcg aacacattga agactttgat tgttatccaa cgaataaggt tgaaatttac
      481 aaccgttggg gagttctagt ttatgaaact acaaactacg ataacgttac aagaagattt
      541 gaaggtatct ctgaaggaag agtcacagtt agtaaatcat ctgaattacc aacaggaact
      601 tacttttata ttattcagta cacaacgact gaaggaaata cagttagtaa aaacggatat
      661 ttatacctta ctagataata aaaccctact aataaacctg aggagtaaca tcctcaggtt
      721 aaaaaaagaa aataaatgaa aacaaaaatt ttaattttcg cgttgatgtt aacatgttac
      781 tgttcatttg ctcagcaaga ttcgcagttt acccaataca tgtacaacac cataaatgta
      841 aatccagcct atgcaggatc tagaggagtt atgagtattt ttggattaca tcgtactcaa
      901 tgggtaggat tagaaggtgc tccaaccact aatgccttct ccataaacac tcctataaat
      961 gatagtaatc taggtttagg tatatcattt ttaaatgaca aaattggtcc aactaataac
     1021 aatacgatta cggccgactt atcatatacc attcaaactt ctgaaaccta taaactgtct
     1081 tttggaatta aagcctctgg aaatatattt aatttagatg ttaataagtt aaatccacaa
     1141 gatgctgatg atcccaactt acaaaattac aacagtaagt tttcaccaaa ttttggagct
     1201 ggtatttatt tacattcaga caaattgtat tttggagttt cggtacctaa ctttttacaa
     1261 gacaataaat acaatgataa ttctgtaacc gtatttaaag aaaaaatgac tttttacact
     1321 attggaggat atgtatttga cctttctcca gatgtaaaat tcaaaccggc tttcttaaca
     1381 aaagttgtaa caggcgctcc tttacaagta gatttatctg gaaatttctt gttgtttgac
     1441 aaatttatgc ttggtgcagc ataccgttgg gatgcagcaa tcagtggaat ggcaggattt
     1501 caagtaacgg atggtttatt cataggatat ggatatgata gagaaactac aaatcttaga
     1561 aattataact caggttctca tgaaattttt attagatttg aattattcaa cagagttaaa
     1621 aaattagttt cacctagatt cttctaatac acaacaatta tgaaaaataa aattatatat
     1681 acagcacttt tactatttat cgcttttaat agtttttcac aaactgttgg taaagagaaa
     1741 aaaggaaata aagattatga acaatatgcc tacattgatg ccatcaagac gtatgagcgt
     1801 ttgtatgaaa aaggatataa atcaccagac atgttgttaa agttaggtaa ctcctattat
     1861 tttaaagctg atttagaaaa agcagcaaaa tattatgatg agctttacac aaccaatcct
     1921 gaccaagaag cagagtatta ttatcgctat tcacaatctt taagaggtat taaagagtat
     1981 aaaaaagctg atgaaatgat gttaaagttc aatgcaaaaa gtggcaatga ttctcgtgca
     2041 gcattattta atcaaaacaa agattattta gctgaaatca aaaggaactc aggaagattt
     2101 aaattagaaa atgctggaat aaattcaaag tattctgatt acggaactgc ttttgttgat
     2161 gataaagtag tattctgttc tgcccgtgat actggaaatt tctctaaaag aattcacaca
     2221 tggactggtg aatatttcac gaatctatat agtgcaaagt tagcggaaga tggctctctt
     2281 agcgatgttc aaaaatttgg aaataaatta aacagcaaat atcacgaaga tacacctgtg
     2341 ttttctaaag acggaaaaac tgtttatttt acaagaaaca actatcttga caaaagagga
     2401 tatgatgatg gaaaagttac acttttaaaa atctataaag caaatttaga tgaaaaagga
     2461 aaatgggaaa atattactcc attacctttc aatagtgata gctatcaaac tgcacatcct
     2521 actctatctc cagatggtaa aatgatgtac tttgtatctg atatgccagg ttcattagga
     2581 aaatcagact tatacaaagt tgccatttca gaagatggaa catttggaac tccaataaat
     2641 ttaggtaaaa ctataaacac tgaaggtaga gaaacctatc catttgttac tgcaaagaat
     2701 gaactttatt tttcttctga tggtcatcct ggattaggag gtttggacat ttttgcagca
     2761 aaaattccag aagatggaaa ctttacaaac atccaaaaca ttggtgaaga agcaaatagt
     2821 cccaaagatg atttcgcttt cattatcaat acagatacta aaagaggttt cttaagctca
     2881 aaccgtgatg gtggagaagg aaatgatgac atatacaaat tccttgaaac aagagaatta
     2941 tttattgaac aagctatttt tggtgtagtt actgatgcta aaactaacga agttttacca
     3001 aatactaaaa ttacattatt ggatgagaaa ttcaataaaa ttgcagaaac cactacagat
     3061 gagaaaggtt attatgaatt cttaaaacta gaaccaaata ctaaatacta tttgagaacc
     3121 gaaaaacctg aatatgacgt taaagaaatt cctgtaataa ctggtaaaga agatggtaga
     3181 actgaagtaa atattgaact tgataaaaca gcacaaccaa ttcctgttgg aggagatatt
     3241 gctgatgtat tcaaaatcaa cttaatctat tttgatttag ataaatggaa tattcgtcca
     3301 gatgcagcca ttgatttagc aaaagttttg gatgtacttg aacaatatcc aacaatgaaa
     3361 attgacattc gttcgcatac agatagccgt gcatcacacg catataatga aaaactatct
     3421 gaccgaagag caaaatcaac tatggcttgg ttagtttcaa aaggaatttc aaaagaccgt
     3481 ttgactgcaa aaggttatgg tgaaacacaa ttagtaaaca aatgtgctga tggtgttcaa
     3541 tgttcagaag ctgaacatca attgaataga agaagtcaat ttatcattgc tgaactataa
     3601 ttaaaataaa ttataatcaa atcttaaaaa gcactcagaa atgagtgctt ttttttattt
     3661 ttgcgactat gaagataact aagcttttta aagatttttt cgatagcgaa aaagtcggtg
     3721 gatttctact tataatttgc actataattt cattactatt cgcaaattca tttttttcaa
     3781 atgaatacct aaacgtttgg caccatccgt ttgctggaaa gcctgttgaa tattggataa
     3841 atgatggatt aatggctatt tttttcctac ttataggttt agaattagaa cgtgaaattt
     3901 atattggtga gttatctaac tttaaaaaag catcattacc catctttgct gccattggtg
     3961 gaatgttagt tcctgcaggc ttgtatctct ttctaaatta tggaactgat acccaatccg
     4021 gtgctggtat tccgatggct acagacattg cttttgcttt gggtgtttta tcactattag
     4081 ggaataaagt accaacatcg ctaaaggttt ttttaacagc attagctgtt atagatgatt
     4141 taggtgccat tttagttatt gcaatctttt ataccaaaaa tttagacttt ggaaatctat
     4201 ctattgcatt aggtatattt gcttttttgt tagttctaaa ccgattaaaa gttagaaacc
     4261 ttattcctta tttggtgctt ggagttttca tgtggtactt catgttaaat tctggagttc
     4321 atgctacgat tactggagtt ttattggcat ttgcaattcc ttttggaaat ggttcaaaaa
     4381 aatcaacttc ctatctacta caaaatgcat tgcacaaacc cgtaacttgg ataatacttc
     4441 ctctttttgc aatggctaac actgcaataa ttatagattc agattggcac tatgcattaa
     4501 ctcataaata tacaattggt atagcacttg gattaatcgt tggaaaacca attggaattt
     4561 cgttgtttag ttatttttca gtaaaaatga aactctccag tcttcctgaa gatttgaatt
     4621 ggaaatccat tattggagtt agttttcttg gaggaattgg ttttacgatg tcaatattca
     4681 taacactatt agcgttcaat gatattgacc acattagcca ttctaaaata atgattttac
     4741 tttcgtctat tacagtagca ctaattggtt tgctcttctt aaaatcaata ttgaaaaaag
     4801 attatattga tgaagtttct aatgataatt aatttaataa gtctaatacc attttttctt
     4861 tttgaaataa tatatcattc ctaataagag aacaaacata caagccaaaa tcacaaaata
     4921 accatatttc caatggagtt ctggcatgtt atcaaaattc attccataaa cgccaacgac
     4981 aaaagttaat ggcataaaga aaaccgaaac aactgtcaaa gttttcatta cttcattcaa
     5041 tttctggttt tgagttgaaa aataaaagtt agtagcactt tctaatgata gtagatcata
     5101 ttctatttgt tccaaaagtt ctattgattt ttgatgtaat cttgaaaaaa aactaaaggt
     5161 atcgtcttca attacattaa aaacattgtc ttctctcata cttttaatcg aaaacaaaga
     5221 atctctgagc ggaataattg aacgtttcaa aaaattaaaa ttatcgcgat gcttttcaat
     5281 tttctctaaa atttgaagtt gtgtgttggt ctttgttaaa ttgattaatg cctctactct
     5341 attctcctca ttttccaatg tgatgtaaaa attctccatc actgcatcaa gtaataaata
     5401 caataaataa tcagattttt tatctctaac caatcccgaa tgtgttctaa ttctttctct
     5461 aacatgcata aaaaaatcac ttcgtttttc ttgaaatgat actaagattt ctttttttaa
     5521 taaaaaacta atttgttcca cttgaatatt atccgagttt tctactggca aaatagattt
     5581 tatattaaag aaaataacat cttgatactc ttcaagctta gagcgttttg ttgtgttaac
     5641 aacatcactt aacataaaat tatctacttt caaaaaatca ccaatcgatt cgattattga
     5701 ttcgtcattt aaaccatgaa gatttaacca attattctta gaaacatcaa tacatttctc
     5761 taattgagag actttaaaat cattgaattc tgttaagtca ttagaatcat aaacaaacag
     5821 ttgcaactct gccaacgttt cttgatgaat tccagtatat tcaagtagtt tggtttgaac
     5881 ctttcttcct tttttgtatt ttatctttcg cactctttgt aaatttgtat aaatttaaga
     5941 ataattactt tttaaaattt atttttacca aaaatcaaat catgcaaacc ttaattatta
     6001 acataaaaga attacttcaa gttagagaaa ctcatattga taaagtttct ggagctgaaa
     6061 tggctatttt accaagtata aaaaatgctt tcttattgat tgagaatgat agtataatag
     6121 actttggaga aatgaacaat tgtacaaatt ttgacaataa gaaaatcatt gatgctaaag
     6181 gtaaaattgt cttgccaact tggtgcgaca gtcatacgca tttggtttat gctggcaacc
     6241 gtgagcaaga gtttgttgat agaattaatg gtttgagtta tgaagaaata gccaatcgtg
     6301 gtggtggtat tttaaattcg tccaaaaagc taaacgaaac tagcgaagaa gaaatctaca
     6361 accaatctaa acttaggttg gaagaaatca tgcaacaagg aactggtgct gtagaaataa
     6421 aatcaggtta tggtttaact gttgatggtg aattaaaaat gctacgtgtt attaaaaaat
     6481 tagcccaaaa ctatccaata acaattaaag ctacttttct tggagcacac gcttttccgt
     6541 tagaatatag agaaaatcat gcagcatata ttgatttaat tattaacgaa atgcttccac
     6601 agattgctga agaaaaatta gccgattata ttgatgcctt tctagaaact ggctatttct
     6661 cggtagaaga aacggaacgc atcatgttgg caggtaaaaa gtatggatta ttggcaaaaa
     6721 ttcatgtcaa tcagtttaca gccattaacg gtattgaagc ttgtgtaaag cacaatgcct
     6781 tatccgttga tcatttagaa attgttaccg atgaagatat caaagtatta aaaaatagtg
     6841 aaaccatgcc agttgctttg ccaagttgct cctattttat aagcattcct tatactccag
     6901 cacgaagatt gatttccgct ggtttacctt tagcattagc aaccgatttt aatccaggta
     6961 ctacaccgtc tggtaacatg aattttgtag tagcgactgc ttgtatcaaa atgaaaatga
     7021 cacccgaaga agccatcaat gctgctacta taaatggtgc ttatgctatg ggaatttctc
     7081 aaacacatgg tagcattacc aaaggtaaaa aagccaattt cataattaca aaaccattga
     7141 attcctttta tgaaattcct tatctgttta cttcaaacct gattgaaaaa gttttcatta
     7201 atggaaactg tatataacaa aaaaagtctc gaaattatcg agacttttta ttaattattt
     7261 taattgatta tcgcaaagta aaactacttt tcgaaaccaa ttcatcttta tcaaatacat
     7321 tcacaaaata agttccttta gcaaaatctt ttcctggtaa ttgctccgaa acctcaactg
     7381 ttttattttg atatgcaaca ttagtagtaa aactatatgt taaagaagta tcgccaaacg
     7441 aaatagttgc tttatctcca agaacattgt tcttagagtc aattacttga acataataaa
     7501 ctttatcgcc agatttagca attttatttt cagcaattgt aaatttaact tccaacatgt
     7561 ctgctctact ggctttgtct gtagaaattt gttttcctga acttcttact ttataagcag
     7621 cagttttcaa gttggtaact gaaagttttt gtcctttttc aaccgttttt gccaattctt
     7681 cattttggcc tacaagcact tggttgtaaa tttttgaatc ttcaagaaca acttttgtac
     7741 tatctaaatt agttgtcaat tgttggtttt gagttttcaa tacttcaact tcagccatta
     7801 agttcttcat tttttgctct aatgctttgt actgatctct gtattttcta agagaagcat
     7861 tatcgccttt tgaagtcttt aattcttgaa ttagtttttc aactttatcg cgttcagcaa
     7921 ttaattcatc agaaatagat gtgttttcag aaatggctgc atcataagaa gtttttaatt
     7981 ctgccaaatc ttttaagaca tcttctttct ccgattttgt aaccgtaaca atgttttgca
     8041 tcgtttttgc atctgaagtc aatttaaaaa tataaatcaa acttccaacc aacaataccg
     8101 ctaagatggc tactattgat ttcaatttag aattgctatt ctgattttcc ataaaaaagt
     8161 tgttttagtt taaacaaaag taataatatt taacataata acgtaagata tacatttata
     8221 ttatattttt gggaaaactt taacttgatg gaaaaactaa ttcctttttc tttaaatgat
     8281 ttagctaaag caactaatca cagaagtggt gaactaaaat ttggtgaaaa gatgcaaact
     8341 atcccaaaag atatttcgcc tttagagtat ttaaaaaatt gtgacgccaa gtttgtttta
     8401 ataggcgttc ctgaagatgt tggagtaaga gccaactatg gaagacctgg agcagcaact
     8461 gcttgggaaa gtgcaataaa aggcatagct aacattcaac acaaccgttt ttgcaaagga
     8521 agtcaaattc tagttttagg aacacttgat gtttctgagc aaatggaaca agcaaaagaa
     8581 ttaaagttta atgacactca tgatagaaaa aaacttaatg agttggttac acaaattgat
     8641 aaagatttag cacatattat tagttgtatt gtaaaaaaca ataaaattcc aatcatcatt
     8701 ggtggtggac acaacaatgc ttatggtaat atcaaaggaa cggcactcgg attaggaaaa
     8761 ccaattaatg tcatcaactt tgatgcgcat tctgatttta gaattctcga aggaaggcac
     8821 agcggcaatg gtttttcgta tgcatttgaa gaaggtttct tgaaaaaata tttcattttt
     8881 ggattacacg aaaactatac ttccaaaaat gtattggata atttaaaaaa acttgaagat
     8941 cgaatcaatt ataataccta tgatgaaatt ggtattcgaa aagaaaaagt atttgaggaa
     9001 gaattaaata aagctttaaa ctttataaaa tctgaacctt ttggaattga aattgattta
     9061 gatgctttac caaacattcc tagtagtgcc atgactttaa gcggtttttc ggtagaagaa
     9121 gttcgtaatt tcatcaatca ttttggaaaa cacaaaaact caaaatacat tcacatttgt
     9181 gaaggtgcac ccgatcttgg agacgaaaag aacagccatt taattggtaa attaataggg
     9241 tacttcgtta ccgattttgt gaaatcgaaa agagaagcga ccaccacaaa tggtaaaaat
     9301 taaaaacctt atctttgccg tgacttacaa ctaaagtaag tactcattat gttattcgaa
     9361 gatctatcat tatcaaaaag tattcaaaga gccgtttttg aagaaggata tacccaacca
     9421 acaccaattc aagaaaaagc aattcctcat attttagcag ggaaagattt agttggttgt
     9481 gcacaaactg gaaccggaaa aacagcagcc tttgctatcc caattattca caatctccac
     9541 agaatggtag gctcgtcaaa gaaaacgaaa gtcattcgtt gtcttgtggt tactcctact
     9601 agagaattgg cagttcaaat tggcgaaagc tttgacacct atggaaaata tactaaccta
     9661 agacaactca ctatttttgg aggtgtttct caagtaccac aagtagatca gttaaaaaaa
     9721 ggtattgata tactgattgc cacacctgga agattattag acttacataa acaaggtttt
     9781 attgacttcg accatttgca ttatttggtt cttgatgagt cagatttgat gctcgatatg
     9841 ggttttatca atgatgtaaa gaaaattgtc aaactcgttc ctaccaatcg tcaaactttg
     9901 ttgttctcgg caacaatgcc attagcgatt agagaattat cagaaacttt tttaaacaaa
     9961 cctgagtatg tatcggtaac accggtttct tctacggcag aaattattga acaacaaata
    10021 tactttgtag ataaagaaaa caaacgtggc ttgttatacc atttaatacg caatgaaaac
    10081 ctaaagaacg ttttggtttt tgttcgaacc aaacatggtg ccgacaatgt ggtaaaagct
    10141 ttgaaaaaac atggtgtaaa tgccgaagct attcatggca ataaatcaca aaacgcacgt
    10201 caacgtgtgt tggatgagtt taaaaacaaa gatatttctg ttttggtagc tacagacatt
    10261 gcagctcgtg gaatagatat tgaaagtttg ccttttgtca ttaatttcga cttgccaaac
    10321 attccagaaa catatgtaca tcgtattgga agaacaggtc gtgctggtaa cgacggtatt
    10381 tccatttcat tttgtggtcg cgacgaagaa ccctattgga aagacatttt gaaattgata
    10441 aaagtcaacg ttaaaacgat gaaagaccat ccgtttccat ggaaagaagg tactccaaat
    10501 cctgaaacca aaccagattt acgaaataag aataaaaata aaacggcaaa cacttcgaga
    10561 aaatcggaag cctctaagaa aaataaaaag cgatggtatt aatcatcgct ttcttttttg
    10621 ggtatatggg ttgatatgat ttgatataac ttcgtgggtt caaaaggttt gattaagata
    10681 tcattcatac cactagcata tacttcttcc gaaacttctt gtttgtcaaa agcagtcaac
    10741 gctataattg gcatcgtata tcctttttct ctaatctttc ttgtagtgtc aaaaccatta
    10801 atcaacggca tgtttatgtc catcaatatt aaatcaaaat gttctcgttc tagagccacc
    10861 aaagcagcat aaccatcatc tacaatggta catgtaagat tgctattctg aatgattttc
    10921 ttagtaacca attggttgat tttattgtct tcaatgacta atatcgtata tgtatggtgt
    10981 gatgacaaat caacttcaat ttcattgatt aacgctacgc attgtttttc atcgtggata
    11041 aaacctattg taaatgtaaa tgaggtacca acattctctt cgctttcaag atgaatttca
    11101 ctcttaaaca attttattaa actggtaaca atagaaagtc ctaatcctgt tccttgatag
    11161 tcttcttcct tgcgttcaat ctgtacaaat ttatcaaaaa tcacttgctg gtgttctttg
    11221 gcaattccaa caccattgtc aattacttta aacgtaatat aatacatagt atcttctact
    11281 cgctccaaat cggcagtgat gataacttct ccattcttgg taaacttcaa tgaattgctc
    11341 accaagttca taataatttg tgataaacgt aacttgtcgc caattaaaaa ctctggtatg
    11401 gcggtatcta tttcaataat aagcttattg ttgtttcttt ctgcaatata ttctatcgaa
    11461 ttacgaatac catctatctc gtcggtcaaa ttgaaaatca ggttttccaa aaccactttt
    11521 cgctcttcga ttttactgat ttgcaaaatg tcattcacta acgacaataa gtattttgct
    11581 gagaatttta gtgaattcaa atgcggacta tttgccaatt ctttgtgttc gtctaataat
    11641 atattagtaa tcccaataac accatataaa ggtgtgcgca gctcatgagt aattgtcgat
    11701 acaaactgtg ttttcatttg tgaagcttct tctgcttttt ctttggctat actaagtgct
    11761 tcgttcgtat tctttaattc taaattcagt ttttctcgtg tcttgatact tttataaagt
    11821 gttagcaaca ataataacaa tgtgataaca atcagcataa aaagcaccaa aaccaatttt
    11881 gaagtttcaa ctatcttgga gtttttttca ctttgtagtt ctactttctc caattgtatt
    11941 ttatactcgt caagttctat atctagtgct tgtttctcaa tgctgtccat cttttcatca
    12001 gagtaaagca cattatataa tgagtcgtgc ttagcatcat agtgttcttg cttggtctgc
    12061 tcgttaaaat acccaaaatg cttggcaaga ttttcgtaaa cattcaacaa attagaacta
    12121 aagtggttct tttcggctaa atctttggct ttaaaataaa attcttcggc attctctttt
    12181 ttattatcat gggtattttt tatacccatc agcacataat atgatagctt tatttcatcc
    12241 gtattatttt taaatacagc ttctttaata gttttaagaa tactatcagc tttctggtct
    12301 tcatttgttg ccaaatatgc actagcaaga ttcaaattga tatgaataat ttgattttcg
    12361 tcctttaact tttttgcaaa ataaagtgct tttttataat agcttattcc tttatcggtg
    12421 tcaatgttgt taaagtaata agcacttcca agattattat atatccaatt attaagggta
    12481 tcattgttta tttgtcgtgc aaatcggagt gctttttcat aaaactcaat agcacgtttg
    12541 gaatcattgc attcatcata tatcacacca atggtgttat aggactgtgc tatataggaa
    12601 ttgctattta tttcaaaaga cttaatcaaa gcagcttttg acgctctcaa agctttgtca
    12661 aaatgggtgt tttcgaaatg atgcacagct tggtctaaag ctgtttttat tgtttgttct
    12721 tgttcctttt ttgtcaaagc aacttcttgt gccaatgata ggacattaac aaacaataac
    12781 aaaaacaaaa ttctatatct gttttggtaa atcatagggc aagttcaatg ctcaaatata
    12841 aagtatttta caaaaaaaaa tcctgaaaat ttcaggattt tttaaagaat tgttaatttc
    12901 taatcaactt tctatatttt attctttttg gtgtcaaatc gccacccaat cgtttgcgtt
    12961 tgttctcttc atattccgaa aaactacctt caaagaaata tacttccgaa tctccttcaa
    13021 atgccaagat gtgtgtacag attctatcca aaaaccaacg gtcgtgactg atgactacgg
    13081 cacaaccagc aaaattttct agtccttctt ccaatgctct tagggtgttg atatccaaat
    13141 cattggttgg ctcatctagt agtaacacat taccttcttc tttcaaagtc attgccaagt
    13201 gcaaacggtt gcgttctcct cctgaaagtg ccgaaacctt tttgttttga tcgcttccac
    13261 caaagttaaa acgcgaaaga taggctcttg agtttacttg tcgtccgccc attaaaatta
    13321 actcttgtcc atcacagaaa ttttcccaga tggatttgtt tacatcaata ttagagtgcg
    13381 cttgatctac ataggcaatc tttactgttt cacctacatt aaattctcca gagtcggttt
    13441 gttgttctcc cattatcatg cggaaaatgg ttgatttacc agcaccattt ggtccaataa
    13501 tcccaacaat tcctgcttgt ggcaaggtaa agtttaggtt atcatataat aacttgtcgc
    13561 caaatgcttt tgctacgttc tta
//
LOCUS       DAOOBH010000060        13426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_5929_length_13426_cov_9.045472, whole genome shotgun sequence.
ACCESSION   DAOOBH010000060 DAOOBH010000000
VERSION     DAOOBH010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13426
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5929_length_13426_cov_9.045472"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..743)
                     /locus_tag="PK218_07265"
     CDS             complement(237..743)
                     /locus_tag="PK218_07265"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HPW98341.1"
                     /translation="MMTQDEGRFGRVNIPRYLWAPMGIRPMVPRQVIREAFYVYSAVC
                     PSSGELCSLLLPYANTEMMNLFLKHVSNEFSDKEIIMLVDGAGWHRSKDLVIPSNIYF
                     IVQPPYSPELNPTEHIWDEIREKYLHNRIFASLNETIEKVSNGIRELSQKKDLIKSMT
                     LFPYLNVL"
     gene            complement(788..1291)
                     /locus_tag="PK218_07270"
     CDS             complement(788..1291)
                     /locus_tag="PK218_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPW98342.1"
                     /translation="MGKVTKVKNHLSNDELEKKIKETVGFWRVQKWLIIYNAQNYPRT
                     SEEIANHLAVSKALVNKTVSEYNRFGVAAIETVGRGGRKNAYMSVEQEKEFVGSYISR
                     SQKGQIVTVQEIQEDFEKAVGKKVNKTTIYRLLKRHGWRKVMPRSFHPKRDKEKQTAF
                     KKTSRTK"
     gene            complement(1795..2169)
                     /locus_tag="PK218_07275"
     CDS             complement(1795..2169)
                     /locus_tag="PK218_07275"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HPW98343.1"
                     /translation="MENQEKSSCLCGVSEYMVLACSGACDLGQITDIVARRLRDNGVR
                     KMNCLAVAGAGIEKSIEDFKKKNILMLDGCPIDCGKQILDKAGFKDYKYMRVTDLGFK
                     KGQTPINEDVIKKVYDTAEIIY"
     gene            complement(2180..3259)
                     /locus_tag="PK218_07280"
     CDS             complement(2180..3259)
                     /locus_tag="PK218_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98344.1"
                     /translation="MTISKLAFLALLFFTGNLVGFSQTDTTGTHKGKPIIQVFGNFDY
                     NATQGTQKKYGFWFGRAHFGYDYQFNNHFSGKIIIDAGRPTTVGQIEVTDTTGLNLNT
                     SNTSKEGSYYTMTLKFASLEWKPNEHIKIQAGGVLQNHYITQEKFWGYRYVAPTFQDK
                     YYGIPSSDLGIIAYFKINEKLGFDIAFTNGEGFRFDQDAYGDVKIASGFDYKPFKGLQ
                     TRFYYDYTQSNNPLKPTEQKLYSFFAGYKFKQKFRIGGELNYRENNLNINNHDLFGYS
                     VFGAYSIFKQIELFARFDNLQANTINNDIHNWYYENTGKAYISGIHYNAIEGVNISLN
                     YQGWQPDDVSINFQHHVVLSFEYKL"
     gene            complement(3272..4615)
                     /locus_tag="PK218_07285"
     CDS             complement(3272..4615)
                     /locus_tag="PK218_07285"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPW98345.1"
                     /translation="METKKEIKILIWIAVIFGVAFFLPIESARFNTAIAATFDLVKWY
                     AREHVVLCLLPAFFIAGVISVFVSQGSVLRYFGANAKKWLAYTVAAVSGTVLAVCSCT
                     ILPLFSSIHKRGAGLGPAIAFLYSGPAINILAIILTARILGFEMGVARTIGAISFSVI
                     IGLIMAFIYRKEEKIKKEEQMNIQLPPEKRPMWQTSFHFFILVLILVFANWGKPTADD
                     VSSTWYYLWAYKWYITGFFALLLGYSLIRILKIKWQWVLIAAIATVASALLSSNLIEN
                     AKLAPLVPMLVGIASLSIITLFDKNEDENREWTISSWGFAKQILPLLAIGVITAGFLL
                     GSTHDGQTIAGVIPNEWIQWLVGGNSIFSNFFASVVGAFMYFATLTEVPILQGLIASG
                     MGKGPALALLLAGPSLSLPNMLVIRGVLGTQKTVVYVSLVVVMATVSGLIYGSLF"
     gene            complement(4717..5802)
                     /gene="arsB"
                     /locus_tag="PK218_07290"
     CDS             complement(4717..5802)
                     /gene="arsB"
                     /locus_tag="PK218_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPW98346.1"
                     /translation="MPSKKRLNFLDRYLTLWIFLAMFIGIFSGWLFPEIAKFWESLKP
                     TPESTTNIPIAIGLILMMYPPLAKVKYEELGDVFKNFKVLGLSLVQNWIIGPVLMFAL
                     AVLFFKLFPGQDNANLPYFYGIVMIGLARCIAMVIVWNDLAKGDTQYAAGLVAFNSIF
                     QVIFFSVYAYFFVAVLPGWFGIPTNSIVENITIWQIAESVFIYLGIPFIAGFLTRYIL
                     IRVKDKTWYHTKFIPKISPITLIALLFTIIVMFSLKGEYIVKIPLDVLLIALPLLLYF
                     LIMFVVSFFMSKKVGANYGQSVTLSFTAASNNFELAIAVAVAIFGMHSGEALAAVVGP
                     LVEVPVMLALVNLAFFFQKKYFNKSIP"
     gene            complement(5807..6223)
                     /locus_tag="PK218_07295"
     CDS             complement(5807..6223)
                     /locus_tag="PK218_07295"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPW98347.1"
                     /translation="MKVLILCTGNSCRSQMAHGFLQSFDKKLQVFSAGTEPAKQINAK
                     AVQVMLEIGIDISHHTPKHVEQYINETWDYVITVCGGANETCPAFFGTVKNRLHIGFE
                     DPSEAIGTNEFIMSEFCRVRDEIKTEFQKFYNNLKI"
     gene            complement(6235..6936)
                     /locus_tag="PK218_07300"
     CDS             complement(6235..6936)
                     /locus_tag="PK218_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HPW98348.1"
                     /translation="MEFLQNILDGSNFPILSAFILGLMTAISPCPLATNITAIAFIRK
                     NIENKRKVFINGLLYTLGRAITYVSLGVILFFGASKFHVASFFQSNGEKFLGPLLLII
                     GIFMLDFIKIKFPGLGNITDKFQNSEKQGSGISALLMGILFALAFCPYSGVLYFGMLI
                     PITISSTSGLILPLIFAIATGLPVIIFAYLIAFTLSGVGKLYNGLKVFELWFRRVVSA
                     IFILVGMYYIYIFFF"
     gene            complement(6942..7391)
                     /locus_tag="PK218_07305"
     CDS             complement(6942..7391)
                     /locus_tag="PK218_07305"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HPW98349.1"
                     /translation="MKAKILFLFIATSIFVACNQGSQKTNNEQVKNEITQNPNLKLQI
                     YYFHATHRCPTCNSIEANIKQVLESNFKNEIEQGIINFTVLNVEDTENKNLAEKYQAT
                     GAALHLVDIENRAEKDNDLTKYAFSYSRKQPDVFLKGMSDTINYFIK"
     gene            complement(7412..7729)
                     /locus_tag="PK218_07310"
     CDS             complement(7412..7729)
                     /locus_tag="PK218_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HPW98350.1"
                     /translation="MAKKIEFSQDILEIAKFAKAMSHPVRVYILMKLSEMDACCYSGN
                     LIEELPVGRSALSQHLKELKFAGLIQGEIESPYIKYCINRENFEKAKIMCHAFFGKDI
                     KNK"
     gene            complement(7722..7973)
                     /locus_tag="PK218_07315"
     CDS             complement(7722..7973)
                     /locus_tag="PK218_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPW98351.1"
                     /translation="MKMNIKILGTGCPKCKTLERLTTEVVTENSFDAEISKVEDIVQI
                     MNYGVMGTPGLVVNEKVLISGRVPSKDEIKKLIEKHSNG"
     gene            complement(8065..8376)
                     /locus_tag="PK218_07320"
     CDS             complement(8065..8376)
                     /locus_tag="PK218_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPW98352.1"
                     /translation="MAKEKLTKEQEQLARLAKALGHPVRVAILQMLAKQTCCYHGDMS
                     EILPVAKSTLSQHLKELKESGLIQGTITPPTVKYCINKENWLLAKKMFGEMFIELSKN
                     E"
     gene            complement(8733..11732)
                     /locus_tag="PK218_07325"
     CDS             complement(8733..11732)
                     /locus_tag="PK218_07325"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HPW98353.1"
                     /translation="MTGTCQEEKNLGHNLVAEPQEIYRGGNGFYIGNSNDFNAQFALD
                     EIRFWHFIENTQKEELDKLKRTADWKLKIVERLDRMIKKYGLLRLLRKGLEVEDAHFT
                     LLYQVPMASSSQVVKDNFEKNEFSVTRQVRYNTENQREEMDMVVFVNGLPIATLELKN
                     AWTGQTAKVHGIKQYKYDRDIKQPLLNFGRCLVHFAVDTDEVYMCTKLDGANSFFLPF
                     NKGNNNGKGNPPNPFGHKSSYLWEEIFTRESLANIIQHFVRFDGSPSDALSKRTLYFP
                     RYHQLDVVRKLLSHASKNGVGHTYLIQHSAGSGKSNSITWAAYQLIETYPERDGLPGA
                     KGIQNPLFDSVIVVTDRRLLDKQLRENIKEFSEVKNIVAPAFSSKDLKDGLEQGKKII
                     ITTIQKFPFIVDGIADLSDKRFAVIIDEAHSSQSGTAAGKMNQAMGVGQNEEDEEDAQ
                     DKILKAMQARKMKGNASYLAFTATPKNATLERFGTKQEDGTFKPFHLYSMKQAIEEGF
                     ILDVLANYTTYKSYYEIEKSIEDNPLFDTKKAQKKLRAYVERDKRTIATKAEIMLEHF
                     ISKVYNTKKLKGKAKAMVITQNIESAIRYYLAIKQILKDKGNPFKIAIAFSGKKTVDG
                     IEYTEADLNGFAEKDTRDNFDSDDYRMLVVANKYLTGFDQPKLCTMYVDKKLQGVLAV
                     QALSRLNRAADKLGKKTEDLFVLDFFNEVDDIKAAFDPFYTSTSLSSATDVNVLHELK
                     GTLDDVGVYEWSEVEDFVTKYFDGLDAQKLSPIIDKAANRFNSELDLKDEEKADYKIK
                     AKQFVKIYGQMASIMPYEVLDWEKLFWFLKFLIPKLIVRTKEDELIDELLESVDLSTY
                     GLERTKLNHSIGLDDSATELDPQNPNPRGAHGGEEEKDPLDEIIKSFNERWFHGWDAT
                     PEDQRVKFITISRHIQAHPDFKTKVADNKDAQNRDLAFKKILDDVMGQQRKKELELYK
                     LYAKDESFYQALFDTMKRMIDNPRLGQ"
     gene            complement(11797..12750)
                     /gene="kwaB"
                     /locus_tag="PK218_07330"
     CDS             complement(11797..12750)
                     /gene="kwaB"
                     /locus_tag="PK218_07330"
                     /inference="COORDINATES: protein motif:HMM:NF041623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage protein KwaB"
                     /protein_id="HPW98354.1"
                     /translation="MTLEELRTKIESIVQMEAIGLNVYFLLDTEQGQIIKRANIIENV
                     KTNLVTAYRYSLNQVVANEDLTMIDLSAADDRQTAIYLYDLEERPAVFTYFESIQNDN
                     EEHPIPLFEFDTDNLADLEGYFISIGDFENNVILYRKQMPINLFRQGKIYLVKGHDTQ
                     FDKIDKEFLRIDTKIDVISAEDSIIINNISILERHYEFRAIIEHEAEASLENINGLDI
                     LENIEVLQERVSDTTFARKLSKISTTSPVFNLPTEHIMQFVRNHHILGNEFRYSDDET
                     KILLDTKKSQNFFLRLMNDDFLHSQLTNYDYMTPAKDRLQA"
     gene            complement(12754..13314)
                     /gene="kwaA"
                     /locus_tag="PK218_07335"
     CDS             complement(12754..13314)
                     /gene="kwaA"
                     /locus_tag="PK218_07335"
                     /inference="COORDINATES: protein motif:HMM:NF041622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage protein KwaA"
                     /protein_id="HPW98355.1"
                     /translation="MKNIKLFIMSFWIFFVLLIIVTIDLPICFGANCEFIGFKELFSI
                     RNSVAFASILLLLSGIFFYYEFMKDLEGDLRQPVTIEKIENVNYEYFALLVTIISLVA
                     FDFTSPRGLILLVVLFLILGAIFIKTELFYSNPSFALLGFHIYKADTNNELLKNKIFI
                     TKDKIKSQDKVKYMKISEKVYFTKKV"
BASE COUNT         4090 a         2527 c         2097 g         4712 t
ORIGIN      
        1 aataaaagct cgtacaatac gattgtttga gtatttagtg gaaataagtg cgtatgtaga
       61 ttgataatgg cttttttgtt tcaaaaattt tactttcaaa aattattttc aaaaaatcca
      121 agtatctata aaaaatcttt agaaacttat tggaaaatct aaaattcttt caacttttaa
      181 cacttcaact acgttcagtg catcgctttt aacttttaat accaagttgc gttctattat
      241 aaaacattaa gataaggaaa aagtgtcata gattttatta aatctttttt ttgagaaagt
      301 tctctgatgc cattactaac tttttctatt gtttcattta gtgaagcaaa tattctattg
      361 tgtaaatact tttctcttat ttcatcccat atgtgttctg ttggattaag ttctggacta
      421 tagggtggct gtactataaa ataaatgtta gagggaatga ctaaatcttt cgacctatgc
      481 catcctgcac catctactaa cattattatt tccttatcac taaattcgtt tgagacgtgt
      541 tttaaaaaca aattcatcat ctcagtattt gcgtacggta aaagcaacga acacagctct
      601 ccagaactgg gacaaacagc gctataaaca taaaaagcct ctctaatcac ttgtcttgga
      661 accataggtc gtatccccat aggtgcccat aaatatctag gaatgtttac tctgccaaaa
      721 cgaccttcat cttgagtcat tataacgacc ttattttcat caagattatt tgcttttata
      781 gtttttatta cttcgttctt gaagtttttt taaaagctgt ttgcttttct ttatcgcgtt
      841 taggatgaaa agagcgtggc atcacttttc tccaaccatg tcttttcaat agtctgtaaa
      901 tagttgtttt gttaactttt ttgccgactg ctttttcaaa atcttcttga atttcctgaa
      961 ctgtaactat ttgacctttt tgagaacgag aaatatatga gccaacaaac tctttttctt
     1021 gttcaacact catgtaggcg tttttcctac cacctctacc aaccgtttct attgcagcaa
     1081 caccaaatct attatattcc gatacggttt tgtttaccaa agccttggaa acagctaaat
     1141 gattagctat ttcttcagaa gttcgtggat aattctgtgc attataaatt attaaccact
     1201 tttgaactcg ccaaaaacca acagtttcct taattttttt ctctaattca tcattactta
     1261 aatgattttt tacttttgtt actttaccca taatgttttt ttgcaaagat aacaaattat
     1321 ataatagaaa gcaaattagt ataagaaagc gatatcattg gaatatcgtg taaaaatttg
     1381 ttttatttca gaagcattca tattgtgtaa gagcatctat tatatgtcac ataacgtgtt
     1441 acggcttggc gtagtggggt ttttcagaac taaagttcat acgaagcaca gagttcaaat
     1501 ttaacacaaa gtttcatacg aagaacgtcc gccccattac gccaaaccgc tgttaccgcc
     1561 tggtgttctg tcttccagtc ttgtaaacca ttgttaatat tgagtggcct tgtcattgtt
     1621 atatcacttt tctgtctgtg ttggctcgtg tgtcagagtt ttttattttt tttagtgtcg
     1681 gcaaattttt taaaacaatt tctctcagtg cattggcttt gcaagctctt tgacaaagct
     1741 ttggttgtag cgttggctcg tgtggctttg gcaatgtgct tgcaaatgga ttggctaata
     1801 aattatttca gcagtatcat atactttttt aattacgtct tcattaattg gagtttgtcc
     1861 ttttttaaaa ccaaggtctg taactcgcat atatttataa tctttaaagc ctgccttgtc
     1921 taatatttgt tttccgcaat caattgggca accatccagc ataaggatgt tttttttctt
     1981 aaaatcttca attgattttt caatacctgc accagctaca gcaagacaat tcattttccg
     2041 aactccgtta tctcttaatc tacgggctac aatgtcggta atctgtccta aatcacaagc
     2101 accggaacaa gctaaaacca tatattcgct tactccgcat aaacatgaac ttttttcttg
     2161 attttccatt tttttatttt taaagtttgt actcaaaact caatactaca tgatgttgaa
     2221 agtttattga aacatcgtca ggttgccagc cctgataatt taatgaaata tttacgcctt
     2281 ctatggcatt ataatgtatt cctgaaatat atgcttttcc tgtattttca taataccaat
     2341 tatgaatatc gttattaatg gtatttgcct gtaaattgtc aaaacgtgca aacagttcaa
     2401 tctgtttaaa aatgctgtaa gctccaaaaa ctgaatatcc aaataaatcg tgattgttga
     2461 tatttagatt attttcacga taattcaact ctcctccaat cctaaatttc tgtttaaact
     2521 tataccctgc aaaaaatgaa taaagttttt gttccgttgg ttttaaagga ttgttggatt
     2581 gagtataatc ataataaaaa cgagtttgca atcctttgaa tggtttataa tcaaaacctg
     2641 aagctatctt aacatctcca taagcatctt ggtcaaagcg gaaaccttcg ccatttgtga
     2701 aagctatatc aaagcccaat ttctcattaa ttttaaagta agctattata cctaaatccg
     2761 aacttggaat cccataatat ttatcttgaa aagttggagc tacatagcga tagccccaaa
     2821 atttctcttg tgttatataa tgattctgta atacaccccc tgcttgtatt tttatatgtt
     2881 cgtttggttt ccattctaac gatgcaaatt tcagggtcat tgtataataa ctcccttctt
     2941 tcgaagtgtt tgatgtattt aaattaagtc ctgtagtatc tgttacttca atttgtccga
     3001 ctgttgttgg gcgacctgca tcaataatta ttttacctga aaaatgatta ttgaattgat
     3061 aatcatatcc aaaatgggca cgaccaaacc aaaatccata ttttttttgt gtaccttgag
     3121 ttgcattata atcaaaatta ccaaatacct gaataattgg tttgccttta tgtgtcccag
     3181 ttgtatcggt ttgagaaaat ccaaccaaat tacctgtaaa aaataatagt gctaaaaaag
     3241 ctaatttact tattgtcatt tttctaactt tttaaaatag actgccataa atcaagcccg
     3301 aaacggttgc cattacaaca actaatgaaa cataaacaac tgttttttgt gttcctaaaa
     3361 caccacggat taccaacata tttggtaatg ataaggatgg gcctgccaat aataaagcca
     3421 atgcagggcc tttgcccatg cccgatgcta ttaaaccttg taaaattgga acttcggtaa
     3481 gggttgcaaa atacataaat gcacctacaa cagaagcaaa gaaatttgaa aagattgaat
     3541 taccaccaac taaccattgt atccattcat ttggtattac acctgctatg gtttgcccat
     3601 catgtgttga gccaagtaga aatcctgctg taattacacc gatagcaagt aagggtaaaa
     3661 tttgctttgc aaaaccccac gaagaaattg tccattccct gttttcatct tcatttttat
     3721 caaataaagt aataatgctt aacgaagcta ttccgaccaa cataggcaca agcggagcaa
     3781 gttttgcatt ttctataaga tttgaggata atagtgccga tgcaacagtc gcaattgcag
     3841 cgattaacac ccattgccat tttattttta aaatgcggat taaagaataa ccaagtaata
     3901 atgcaaaaaa tcctgtaata taccatttgt aagcccacaa ataataccaa gtactcgaaa
     3961 catcgtcagc cgtaggttta ccccagtttg caaaaacaag aattaaaacc agtataaaaa
     4021 agtgaaatga tgtttgccac ataggtcgct tttcaggtgg caattgaatg ttcatttgtt
     4081 cttctttttt tatcttttct tcttttctat aaataaatgc cattattaag ccgattataa
     4141 cgctaaatga aatagctccg attgttcttg caacacccat ctcaaaacct aaaatccgag
     4201 ctgttaaaat tattgccaat atatttattg caggacctga atataaaaaa gcaattgcag
     4261 ggcctaaacc tgctccacgc ttatgaatac ttgaaaataa tggcagtatt gtgcaactgc
     4321 aaactgctaa tactgttcct gaaacagcgg caacagtata agccaaccat tttttagcat
     4381 tagccccaaa gtatcgcaaa accgaaccct gactaacaaa taccgaaatt acacctgcaa
     4441 taaaaaaggc aggcaacaag cataatacaa catgttccct tgcataccat tttacaaggt
     4501 caaaggttgc tgcaatagct gtattaaatc ttgcactttc aataggcaag aaaaatgcta
     4561 ctccgaaaat tactgcaatc catattagga tttttatttc tttttttgtt tccataaata
     4621 ttttctttta cttgtttatt ttatttcgta attacatgaa ttaacgaaat gtatttttaa
     4681 aaaattagat taattttctc attcaaattt tatcgtttaa ggaatagatt tattaaaata
     4741 ctttttctga aaaaagaaag ctaaattaac taatgcaagc ataacaggaa cttccaccaa
     4801 aggacccact acggctgcaa gtgcttcgcc tgagtgcatt ccgaagattg caactgctac
     4861 ggcaattgcc aattcaaaat tattacttgc tgcggtaaac gataaagtta cagattgtcc
     4921 gtaatttgca ccaacttttt tactcataaa aaatgaaacc acaaacatta ttagaaaata
     4981 aagcagcaag ggtaaagcaa tcaataaaac atcaagtggg atttttacaa tgtattcacc
     5041 tttcaatgaa aacattacaa taattgtaaa tagtaatgct atcaatgtaa ttgggcttat
     5101 tttaggaata aatttggtat gataccaagt tttgtctttt actcgaatta aaatgtaacg
     5161 tgttaaaaaa cctgcaataa acggaattcc aagataaata aatacgctct ctgcaatttg
     5221 ccaaatagtg atattttcta ctatcgaatt ggtaggaata ccaaaccagc cgggtaaaac
     5281 tgctacaaaa aagtaggcat atacagagaa aaatatcact tgaaaaattg aattaaacgc
     5341 cactaaacct gctgcatatt gcgtgtcgcc ttttgctaag tcgttccaaa ctattaccat
     5401 agcaatacaa cgagccaaac caatcattac aattccgtaa aaatagggta aatttgcgtt
     5461 gtcttgtccg ggaaacaatt tgaaaaacaa tacagcaaga gcaaacatta aaacgggacc
     5521 gataatccag ttttgaacta atgaaagtcc caaaactttg aagtttttaa aaacatcgcc
     5581 aagctcttcg tattttactt tggcaagtgg cggatacatc attaatatta agccgattgc
     5641 aataggaata ttagttgtgc tttcgggtgt tggtttcagg ctttcccaaa atttagcaat
     5701 ttcgggaaac aaccaccccg aaaaaatacc aatgaacatt gcaaggaaaa tccacaacgt
     5761 aagatatcgg tctaaaaaat taagtcgttt ttttgaaggc ataatttcaa attttcagat
     5821 tgttataaaa tttttgaaat tctgttttta tttcgtctct tactctgcaa aactcactca
     5881 ttataaattc atttgttcca attgcttccg aagggtcttc aaaaccaatg tgtaaacggt
     5941 ttttcacagt tccgaaaaat gccggacacg tttcatttgc tccgccacaa actgtaatta
     6001 cataatccca agtttcgttt atatattgtt caacgtgttt gggtgtatgg tggctgatgt
     6061 caattccgat ttcgagcatt acctgaactg cttttgcatt aatttgtttc gctggttcag
     6121 tgcctgctga aaaaacttgt aattttttat cgaacgattg caagaatcca tgagccattt
     6181 ggcttctaca actgtttcct gtgcaaagaa ttaatacctt cataattaaa tttattaaaa
     6241 gaaaaaaata taaatgtaat acatgcctac caaaatgaaa attgcagaca ctaccctgcg
     6301 aaaccacaat tcaaatactt tcagaccatt atataatttc cctacgcctg ataaagtaaa
     6361 tgcaattaag taagcaaaga tgataactgg taaacctgtc gctattgcaa aaattaaagg
     6421 taatattaac cctgatgtgc ttgatatggt aataggtatt aacattccga aatacaatac
     6481 tccactatac ggacaaaaag ccaatgcaaa caaaataccc attaataatg cacttatacc
     6541 actaccttgt ttttcactat tctgaaattt gtcggttatg ttgccaagtc cgggaaattt
     6601 tatttttata aaatcaagca taaaaatacc gatgattaat aataatgggc ccaaaaactt
     6661 ttccccatta ctttggaaaa aagatgcaac atgaaattta cttgctccaa aaaacaaaat
     6721 tactccaagt gaaacatagg tgattgccct tcctaatgta taaagtagtc cattaataaa
     6781 gacttttcgt ttattttcaa tgttctttct aataaatgcg atggcagtaa tatttgttgc
     6841 caaagggcaa gggcttatgg cagtcataag ccctaaaata aaagccgata atatcgggaa
     6901 attactaccg tctaaaatat tttgtaaaaa ctccattgca attattttat aaagtagttt
     6961 atggtatcac tcattccttt taaaaacaca tcgggttgtt tgcgagaata tgaaaaggca
     7021 tattttgtaa ggtcattatc tttttctgcc ctattttcta tatctactaa atgaagtgct
     7081 gcgcctgtag cctgatattt ttcagccaga tttttgtttt ctgtatcttc aacattcaat
     7141 acagtaaaat ttataatacc ttgttcaatt tcgtttttaa aattactctc caaaacttgt
     7201 ttgatattag cttcaatgct attacaagtt gggcaacggt gtgttgcatg aaaataataa
     7261 atttgaagtt taaggttagg gttttgtgta atttcatttt taacttgttc attgttcgtt
     7321 ttttgtgaac cttgattaca tgcaacaaaa attgaagttg caataaataa aaaaagaatt
     7381 tttgctttca tacatttgaa ttttaattgt tttacttgtt tttaatatcc tttccaaaaa
     7441 aagcgtgaca cataattttt gctttctcaa agttttcacg attaatacag tatttaatat
     7501 atggggattc aatttcaccc tgaattaaac ctgcaaattt cagttctttt aaatgctgtg
     7561 ataatgccga ccttccaaca ggaagttctt caatgagatt gccactatag caacaagcgt
     7621 ccatttcgga gagcttcatt aaaatataaa ccctcacagg atggctcatt gctttggcaa
     7681 attttgcaat ctctaaaata tcttgtgaaa attctatttt cttagccatt gctgtgtttt
     7741 tcaattaact ttttaatttc atcttttgaa ggaactcgtc cgctgataag aactttttcg
     7801 ttcacaacca atccgggagt tcccataaca ccatagttca ttatttgaac aatgtcttct
     7861 accttgctta tttcagcatc aaaactattt tcagttacta cttcagttgt caatctttct
     7921 agtgttttgc acttcggaca acctgttccg agaattttaa tattcatttt cattgtctta
     7981 aaatttaaat ttcattttat ttttaaatct gttttacctg ttcgtatttc gtaattttac
     8041 gaacaatgtg gttaaaaaaa acgtttattc attcttcgag agttcgataa acatctcgcc
     8101 aaacattttt tttgctaaca gccaattttc tttgttgata caatacttta ccgttggcgg
     8161 tgtgattgtt ccttgtatca atcccgattc tttcaactct tttaaatgtt gtgacaaggt
     8221 gcttttcgca acaggcaaaa tttcggacat atcaccatga taacaacacg tttgctttgc
     8281 caacatctgc aaaatagcaa cccgcacagg gtgtcccaat gctttggcta acctcgccaa
     8341 ctgctcttgc tcttttgtca atttttcttt tgccatatct ttaaaatgtt ttgcaaagat
     8401 atattatttt tctctatgtt cgtaaaatta cgaataatat ttttctgaga ggtgcgctgt
     8461 ttcgggttac gcccgcatta ctgccaacgg atgggtatat gaaacgtttg gcatttcaaa
     8521 gcgatttcct atcaaaccgt tgcgatgttt attaaacgta cctcccttaa aatcagcact
     8581 atctgccaaa tgttttatat accgtgttgt gccttcgttg tttctttctt caaccgtgcg
     8641 ttggggcagc catactcttt gccaagtttt ggcttgcggg tcggctttgt gcgacttggc
     8701 aatgtgtatg gatgcagcat ggcgttcata ttttattgtc ctaatcttgg gttatcaatc
     8761 attcttttca tagtatcaaa tagtgcttgg taaaacgatt cgtcttttgc ataaagttta
     8821 tacaattcca attccttttt tctttgttgt cccataacat catccaatat tttcttaaat
     8881 gctaagtcac gattttgtgc atccttgtta tctgcaactt ttgttttaaa gtctgggtgg
     8941 gcttgaatat gtctgctgat ggttataaat tttacacgct ggtcttccgg tgttgcatcc
     9001 cagccatgaa accacctttc gttgaatgac tttatgattt cgtccaacgg gtctttttct
     9061 tcttcaccac catgtgcacc ccttggattt ggattttgcg ggtctaattc tgttgctgaa
     9121 tcgtccaagc caatggaatg atttaatttt gttcgttcaa gtccgtaggt tgaaaggtca
     9181 acagattcca atagttcatc aatcaattca tcttctttgg ttctgacaat taacttaggt
     9241 atcaaaaatt tcaaaaacca aaatagcttt tcccaatcca gcacttcata aggcatgatg
     9301 gatgccattt gtccgtaaat cttgacgaac tgcttagctt ttatcttata gtcggctttc
     9361 tcttcatctt ttaagtctaa ctcgctatta aagcgattag ctgctttgtc tatgattgga
     9421 cttaacttct gggcatccaa tccatcaaag tattttgtaa cgaaatcttc cacttccgac
     9481 cattcataaa cgcccacatc atctaatgtt cctttcaatt catgcaatac attaacgtcc
     9541 gttgcactac tcaaagatgt tgaagtgtaa aatgggtcaa atgcagcttt gatgtcatcc
     9601 acttcattaa agaagtccag aacgaataag tcctctgtct ttttgcctaa tttgtcggct
     9661 gcccgattca agcgacttag tgcttgcact gccaaaacac cttgtagttt tttgtcaacg
     9721 tacatggtgc aaagtttggg ttggtcaaaa ccggtcaggt acttattggc aaccactaac
     9781 attctgtaat catccgaatc aaagttgtcg cgggtatctt tttctgcaaa gccgtttaaa
     9841 tctgcttctg tgtattctat tccgtccacc gttttcttac ccgaaaacgc aatagcaatc
     9901 ttgaacggat tccccttatc cttaagaatc tgctttatag ctaagtaata acgaatagcc
     9961 gattcaatat tctgagtgat aaccattgcc ttggctttgc ctttcagctt tttagtattg
    10021 tacacttttg aaataaagtg ctccaacata atttctgcct tagtggcaat ggtacgttta
    10081 tctctctcta cataagcacg aagtttcttt tgtgcttttt tggtatcgaa caaaggattg
    10141 tcttcaatag acttttctat ttcgtaatag cttttgtatg tagtataatt cgccaaaaca
    10201 tccagaataa agccttcttc aatggcttgt ttcatggaat acaaatgaaa tggcttaaag
    10261 gtgccatctt cttgttttgt gccaaatctt tccaatgtgg cgttcttggg agttgctgta
    10321 aaagccagat aggatgcatt gcccttcatt ttacgggctt gcatggcttt caaaattttg
    10381 tcttgtgcat cttcttcgtc ctcctcattt tgaccaacac ccattgcctg attcattttt
    10441 cctgccgcag ttccgctttg tgaactatga gcttcgtcaa tgattaccgc aaatcgcttg
    10501 tcgcttaggt ctgcaatacc atctacgata aacggaaatt tctgtatcgt tgtaatgatg
    10561 atttttttgc cttgctccaa tccatctttt aaatctttgg atgaaaaagc cggggcgact
    10621 atatttttta cttctgaaaa ttctttgatg ttttcacgca actgcttgtc gagcaatcgt
    10681 ctgtcagtta ctacaattac cgaatcaaac aaaggatttt gaatgccttt tgcaccaggc
    10741 aaaccatctc tttcgggata ggtttcaatt aattgatagg cagcccatgt aatggaattg
    10801 gatttgcccg aaccggctga atgttgaatc agataggtat gtccaacgcc attttttgaa
    10861 gcgtggctca ataattttct aacaacatct aactggtggt atcttgggaa atacagggtt
    10921 cttttgctaa gagcatctga tggactacca tcaaagcgaa caaaatgctg aatgatattc
    10981 gccagacttt ctcttgtaaa gatttcttcc cacaggtagg aagatttatg accgaaggga
    11041 tttggcggat tgcctttgcc gttgttattg cctttgttaa agggtaaaaa gaatgaattt
    11101 gccccatcta gtttggtgca catatacact tcatccgtat ctacggcaaa gtgcactaaa
    11161 catcgaccaa aatttaataa cggttgtttt atgtctcggt cgtacttgta ttgcttgata
    11221 ccatgcactt ttgcggtttg tcctgtccat gcgtttttaa gttccagcgt ggcaatgggt
    11281 aagccgttta caaaaaccac catgtccatt tcttcccgtt ggttttcggt gttgtaacga
    11341 acttggcgtg ttacactaaa ttcattcttt tcaaaattat cctttacaac ttggctgctg
    11401 cttgccattg gaacctgata caatagcgta aagtgtgcat cttccacttc caacccttta
    11461 cgcagcaacc gcaacaaacc atatttctta atcatgcggt caagacgttc aactattttg
    11521 agcttccaat ccgcagttcg tttgagttta tcaagttctt ctttttgtgt gttttctata
    11581 aaatgccaaa accgtatttc atctaatgca aattgagcgt tgaaatcatt ggaattaccg
    11641 atataaaagc cattaccacc tcgataaatt tcttgtggtt cagcaactaa attatgtcct
    11701 aagtttttct cttcttggca agtaccagtc aaggcttttt ctatggccgc ttctagggcc
    11761 tgttcgtttg tttgtgattt catgctgtaa ttaatttcat gcttgtaatc tgtcctttgc
    11821 aggtgtcata tagtcataat tggttaattg agaatgcaaa aaatcatcat tcatcaaccg
    11881 aaggaaaaaa ttctgtgatt ttttcgtgtc gagtagtatt ttagtttcat catcactata
    11941 tctgaactca tttccaagaa tatgatggtt tcttacaaac tgcataatat gctctgtcgg
    12001 taaattgaaa acaggggaag ttgttgatat ttttgaaagt tttctggcaa aagttgtgtc
    12061 ggatactctt tcttgaagta cttctatgtt ttccaaaata tcaagaccat tgatgttttc
    12121 tagtgatgcc tctgcttcgt gttctataat tgccctaaat tcataatgcc tttcaagtat
    12181 tgaaatgtta ttgataataa tggaatcttc tgctgatatc acatcaattt tagtatcaat
    12241 tctcaaaaac tctttatcaa tcttatcaaa ctgtgtatca tgacctttta ccaagtatat
    12301 tttaccttgt ctaaaaagat tgattggcat ttgttttcgg tataaaatga cattattttc
    12361 aaaatcacca atcgaaataa aatatccttc caaatcagcc aaattatctg tgtcaaactc
    12421 aaatagtggt attggatgct cttcattatc attttgaatg ctctcaaaat atgtaaaaac
    12481 tgctggtcgt tcctccaaat cataaaggta aattgcagtt tgtctatcat cagcagctga
    12541 taaatcaatc atggttaaat cttcattggc tacaacctga tttagtgaat atctatatgc
    12601 tgtaacaaga ttagttttaa cattttcaat gatgtttgct cttttgataa tttgaccttg
    12661 ttcagtgtcc aacaaaaagt aaacatttaa gccaatcgct tccatttgta cgattgattc
    12721 tattttggtt cttaattctt ctaaggtcat attttaaact tttttggtaa agtaaacttt
    12781 ctccgaaatt ttcatgtatt tgactttgtc ctgacttttt attttgtctt tcgttatgaa
    12841 aattttgttt ttcagtagtt cattgttggt gtctgcttta tagatatgaa aacctaataa
    12901 agcaaaggaa ggattagaat aaaaaagttc tgtttttatg aaaattgccc ctagaatcaa
    12961 aaacaaaact accaatagaa ttaaacccct tggtgaggtg aaatcaaaag caacaagcga
    13021 aataattgta accaaaagag cgaaatactc ataatttaca ttttcaattt tttcaattgt
    13081 gacaggctgt cttaaatcgc cttccaaatc tttcataaac tcgtaataga agaaaatgcc
    13141 cgacaacaaa agaagtatac ttgcaaacgc tacagagtta cgaattgaaa ataattcttt
    13201 gaaaccaatg aactcgcagt ttgctccaaa gcaaattggt aaatcaatgg ttactataat
    13261 aagtaacaca aagaatatcc aaaaagacat gatgaaaagt tttatattct tcatattaac
    13321 tcacttttat ttttcccgtt accgcactgt ctatcagggt ggatttcaat tctttcaatt
    13381 tctctatctg ctgctcttgc agttttatcg ccttgtctat tttggc
//
LOCUS       DAOOBH010000061        13207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_6053_length_13207_cov_6.710310, whole genome shotgun sequence.
ACCESSION   DAOOBH010000061 DAOOBH010000000
VERSION     DAOOBH010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13207
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6053_length_13207_cov_6.710310"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2645)
                     /locus_tag="PK218_07340"
     CDS             complement(<1..2645)
                     /locus_tag="PK218_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW98356.1"
                     /translation="MKLAEISIKRPTLIVVLFTILTLGGLFSYTQLGYELIPKFETNV
                     ITISTVYPGASPSEIENTVTKKIEDAIASLENIKKIESKSYESLSVVMVTLISGADVD
                     VSMNDAQRKVNAILGDLPDDADPPSLTKFSFSDMPIITLGAKGKMDEAAFYDLIDKKI
                     SPVLSRVSGVAQVNIIGGQEREIQVNLDATKLQGYGLSIPEVQQIILSSNLDFPTGNI
                     QTRDQKILIRLAGKYKTVDELRNLVVSSKNGIQIRLKDIADVQDAQKIAEKIARVDQK
                     SAIILQIVKQSDANAVAVSEKVMSTIKNLENEYKANDLKLEVAKDSTVFTLEAADSVI
                     HDLLIAVILVAIVMLFFLHSIRNSLIVMVSIPASLIATFIGVYLLGYTLNLMSLLGLS
                     LVVGILVDDAIVVLENIYRHMEMGKSRIRAAYDGTAEIGGTVTSITLVIVVVFLPIAM
                     SSGLVSDIITQFCMTVVISTLLSLLASFTIIPWLSSRFGKLEHIEGKNLFERVILGFE
                     NYLTRFTNWISKLLTWCLDHYKTTFAIVLFIFVSSVALVPAGFIGGEFFASSDSGEFL
                     VQIEMPKEASLEQTNLMTQKAEAYLKSKNEVLTQFTTVGQTSEGFGSSQSTAYKAEID
                     VKMVDKKERENKDEAKVYSAKIKRELQKILIGAKVKTVPVGIMGTAEDATLQLVVTGT
                     DVESAMKFAKLAENELRQIPGTSEIKLTVEDGNPEINVKVDRDKMAALGLTMQTVGLT
                     MQTAYSGNTDAKFRSGEYEYDINIKYNEFDRKNINDVSNLIFVNNAGQQVKLNQFATI
                     TEGSGPSQLERRDKTASVTVQGQNIGVPSGTIVQQWQERLDKLEKPTGVNYIWGGDQE
                     NQSEGFGTLGIALLAAIVLVYLVMVG"
     gene            complement(2677..3744)
                     /locus_tag="PK218_07345"
     CDS             complement(2677..3744)
                     /locus_tag="PK218_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW98357.1"
                     /translation="MKKGIITTIAILGSLALIAFVLMKNKKENQEKIDIVAATNSSVL
                     VKAAEIKTEEISFDFNANGTFEPTQELTFSAEKSGKIVKVLAKEGDVVRVGQVLAIMR
                     GDAINVNAENANSVYQNALMDYNRFENAYKTGGVTKQQLDQARIAMINAKSNLSQANI
                     NVGDTRIKAPINGVINKKFIEVGSMISSMPATALYEIVNVSKLKLNVTVNESQVANLK
                     VGTPINVVTNVYPDKTFNGKITFIAAKADASLNFPIEIEISNNANNDLKAGMYGTAKF
                     ESKQQTQKIMIAPRSAFVGSVSGNQVFVNENGIAVLKKVTAGRIIGDKVEILDGLKDG
                     DVVITTGQINLTNGSKIEIIK"
     gene            complement(3750..5090)
                     /locus_tag="PK218_07350"
     CDS             complement(3750..5090)
                     /locus_tag="PK218_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW98358.1"
                     /translation="MKTKLIIVFLLTFALSIAQEEKPLTLKDAITYALQNKADAKKAK
                     LDVENSEYQIQEVRSRALPQISASGNLNHNPILQTSVIDGSSFGAPGSTIQATFGQKW
                     TSGAGVTLQQALYDQSVFIGLKAAKSTREFYQINAQLTEEQIIERVANAYYQVYVTRQ
                     NLNVIENNLKNTTKVRDIIKGQYDNGLAKKIDLDRIIVRISNINTQRQQSINAISLQE
                     NSLKFLMGMPIETNITIPQTKFEVTPNALSEQPDVTSRTEYLLLKKNEQLLEFQKKSV
                     QAGYYPTLSLTGSYNYIGQGPEMPWFKKPTDGVYWSDYAAVGLNLNIPIFSGFGTRAK
                     VRQAENKLQSIKVDLEETKLSLDLQYDNAKKQIENSIVSINNQKENAKLAQEVLTNTN
                     NNYIQGLASLTDLLDAENALVEAQNNYTTAILDYKLAEIQLIKSKGELKTLINN"
     gene            complement(5099..>5647)
                     /locus_tag="PK218_07355"
     CDS             complement(5099..>5647)
                     /locus_tag="PK218_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW98359.1"
                     /translation="KSVTMDDIANEMGISKKTIYQHFSNKTELVKETASYLFDEITKG
                     IDEIYRGNKNPIEELFNVKNYISDYFKAGNQSTIYQFQKYYPKIHDRLKKKHLEKLET
                     CINKNINRGIKDGLFRNDIDISIISRIYFYGSSNLKDNDYFPIEKFNPEKVQLEFLEY
                     HIRGIGTSKGIIKLEQLLKNQL"
     assembly_gap    5648..5747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5748..5856)
                     /locus_tag="PK218_07360"
     CDS             complement(<5748..5856)
                     /locus_tag="PK218_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW98360.1"
                     /translation="MKDKILEKASKMFMSLGFKSVTMDDIANEMGISKKT"
     gene            5999..6973
                     /locus_tag="PK218_07365"
     CDS             5999..6973
                     /locus_tag="PK218_07365"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPW98361.1"
                     /translation="MHPIKHYQELISNHFHQINLVNEPTNLYQPIQYILSLGGKRLRP
                     VLTLMATEVFDVDCKKALAAATAVEVFHNFSLIHDDIMDDAPLRRGKETVHEKWDINT
                     GILSGDAMLILAYQYFEEYEPKVFRDLAKLFSKTALEVCEGQQYDVDFETRDDVTIAQ
                     YLKMIEYKTAVLVGAAMKMGAIVAETSEENANLIYNFGLNLGIAFQLQDDYLDAFGNP
                     ETFGKQVGGDIIENKKTYLYLKALEFSNDSEKEQLLHLFSIQPNDNTDKINSVKAIFN
                     ATGSSDATQKAIQEFTFKAFETLDKMDISDDKKILLRTFGEKLMNRDV"
     gene            6966..7322
                     /locus_tag="PK218_07370"
     CDS             6966..7322
                     /locus_tag="PK218_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98362.1"
                     /translation="MSSFVAPISTDFLLLEAEKENLYQKLIEQLNKDFNLANEAIDFP
                     KSTTPNELKVQLHEKIYRLIQYKFAEYLNLLYIIDVSESEVKKLNGEDLVELSKQVAF
                     LILKREWQKVWFRNKY"
     gene            complement(7319..7858)
                     /locus_tag="PK218_07375"
     CDS             complement(7319..7858)
                     /locus_tag="PK218_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98363.1"
                     /translation="MKIILNLKRTLFVVALFFFSFISSIYSQKNDSLKTKNEFWEHVQ
                     FGGGLGLGIGNGFTNIAVAPSAIYNFNQYVSAGVGLQYSYLKQKNLFDSHTYGGSIIG
                     LVNPIEEIQLSVELEQLRVNVNYENINLKENFWNTGLFLGAGYRTQNVTIGARYNVLF
                     DKDRNVYGDAFMPFVRVYF"
     gene            7974..10733
                     /locus_tag="PK218_07380"
     CDS             7974..10733
                     /locus_tag="PK218_07380"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HPW98364.1"
                     /translation="MDRFSFLNAAHTEFFADLYEQYLQNPDSVEPSWRAFFQGFDFGM
                     ETYNEEQFSKNTSENQDCSLVSDKLQKEFNVLKLIDAYRTRGHLFTETNPVRERRTYV
                     PTLDLENFGLSTSDLDTVFDAAKILGHQPQTLKEILTHLKNVYCKHIGVEYMYMRRPE
                     VIQWMQEKLNINDNLPNFTVDQKKHILGKLNEAVSFENFLHTKYVGQKRFSLEGGESI
                     IPGLDAVIEAAAEKGVEHFVMGMAHRGRLNILANIFGKATQDIFSEFDGKDYDQEYFD
                     GDVKYHLGLTSERVTTSGKKININLAPNPSHLETVGAVIEGITRAKQDKYFPDDFSKV
                     LPIAVHGDAAVAGQGIVYEIVQMSQLDGYKTGGTIHIVINNQVGFTTNYLDARSSTYC
                     TDIAKVTLSPVLHVNADDTEAVVHAMLFALDFRMEFGRDVFIDLLGYRKYGHNEGDEP
                     RFTQPLLYKIIAKHKNPRDIYAAKLIEDGIIDANYISQIENTYKEKLDENLEASRKKN
                     LTVIKPFMQDEWQGFKQVSDDAMRKKVDTTFDRKKLDQIIETVSSLPKDKKFINKISK
                     IVTDRKTQYDNDVLDWGTAETLAYGTLLTEGFDVRISGQDVERGTFSHRHAVVKVEDS
                     EEEVVLLNTLKDKKGKFNVFNSLLSEYGVLGFDYGYALANPNALTIWEAQFGDFSNGC
                     QIMIDQYISCGEDKWNNQNGIVLLLPHGYEGQGAEHSSARMERYLQLCARHNMYVANC
                     STPANFFHLLRRQMKTNFRKPLIHFSPKSLLRHPLCVSKVDDLVKGQFQETIDDTSVD
                     KSKVKTLVFCTGKFYYDILAERENLGRNDVALVRIEQLFPLPVDQLKDIIASYPNADD
                     YVWAQEEPKNMGAYSFMLMNFDLVKWRLASLKAYAAPAAGSHTRDRRRHADAIRMVFD
                     KNLFR"
     gene            10737..10970
                     /locus_tag="PK218_07385"
     CDS             10737..10970
                     /locus_tag="PK218_07385"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HPW98365.1"
                     /translation="MPTVFYINGYRFFFYSNEHLPKHIHIEKAEKTAKFSLESIELIK
                     SFGFNASELKEIRNLVEENQELLIQNWDEFFNN"
     gene            10951..11187
                     /locus_tag="PK218_07390"
     CDS             10951..11187
                     /locus_tag="PK218_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HPW98366.1"
                     /translation="MSFLTINKSKKAISIAFENSKMIVFLEDGRELSIPLEWFPRLRK
                     ASEEQLNKWRLIGKGEGIHWEEIDEDISIENLLE"
     gene            11218..12432
                     /gene="odhB"
                     /locus_tag="PK218_07395"
     CDS             11218..12432
                     /gene="odhB"
                     /locus_tag="PK218_07395"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HPW98367.1"
                     /translation="MILEMKVPSPGESIKEVEIATWLVKDGDYVEKDQAIAEVDSDKA
                     TLELPAEESGIITLKAEEGDAVAVGAVVCLIDTSAAKPAGNAPAAEAKPAAETPKKEE
                     VKPAPVAEKTYASQTPSPAARKILDEKNIQPSDIVGTGKAGRITKEDAENAVPSMGTP
                     TGGARGSEKTKLSMLRRKVAERLVSAKNETAMLTTFNEVDMTAIYELRNQYKEEFKNK
                     HGVGLGFMSFFTKAVTRALQLYPDVNSMIDGDYKIAYDFCDISVAVSGPKGLMVPVMR
                     SAENLSFRGVEAEIKRLAIRARDGQITVDEMTGGTFTISNGGVFGSMLSTPIINPPQS
                     GILGMHNVVERPIVKNGQIVIAPVMFVALSYDHRIIDGRESVGFLVAVKEALENPTEL
                     LMDNNPKKALEL"
     gene            complement(12506..12859)
                     /locus_tag="PK218_07400"
     CDS             complement(12506..12859)
                     /locus_tag="PK218_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98368.1"
                     /translation="MTVGGQVKTFNTISVTEEIFEAGTPDEYTDLMVLATNDTDTETI
                     SINLEKGVTGTDVIYYLSYTENDNEYYRTASFNTNVTVNDSSKNLKGTFSGTLQDSAT
                     GSTLTVTNGSFDINY"
     gene            complement(12968..>13207)
                     /locus_tag="PK218_07405"
     CDS             complement(12968..>13207)
                     /locus_tag="PK218_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98369.1"
                     /translation="WRKNVAQYALLRMILKEENASFYILKLYMDVKNKKVFFRILSLT
                     SLNMILKQNLRVLFCVMKKYCVSNKNKEFMSYFCI"
BASE COUNT         4328 a         2095 c         2174 g         4510 t
ORIGIN      
        1 ccaaccatta ctaaatatac taatactata gcagctaaaa gggcaattcc taaagtacca
       61 aaaccttcac tttgattttc ttgatcgcca ccccaaatat aattcacacc tgtgggtttt
      121 tctaatttat caagtcgttc ttgccattgt tgtacaattg ttcccgaagg aacaccaata
      181 ttttgtcctt gtacagtaac tgaagctgtt ttatctctac gctccaattg gctcggaccg
      241 gaaccttcag taatagtggc aaattgattt aatttaactt gttgtcctgc attatttaca
      301 aaaatcagat tgcttacgtc gtttatgttt ttacgatcaa attcgttgta tttaatattg
      361 atgtcgtatt catattcacc agatctaaat ttagcatcgg tattaccact ataagcagtt
      421 tgcattgtta aaccaacagt ttgcatcgtt aagcccaaag cagccatttt atcacggtca
      481 actttaacat taatttctgg attaccatct tcaaccgtta atttaatttc acttgtgcct
      541 ggaatttgac gcaattcgtt ttcggccaat ttagcaaatt tcatagcact ttcaacatct
      601 gtacccgtaa caactaattg taaggttgcg tcttcggcag tacccataat accaacagga
      661 accgttttta ccttagcacc aattaatatt ttttgaagct cacgttttat tttagccgaa
      721 tatactttgg cttcgtcttt gttttcacgt tcttttttgt caaccatttt aacatcaatt
      781 tcagctttat aggcagttga ttgtgaagaa ccaaaacctt cgctggtttg ccctactgtt
      841 gtaaattgag ttaaaacttc atttttagat tttaaatacg cttcggcttt ttgggtcatt
      901 aaattggttt gttccaatga agcttctttt ggcatttcga tttgaactaa gaactcacca
      961 ctatccgaag aggcaaagaa ctcaccacca ataaatccag caggaactaa agctactgaa
     1021 cttacgaaaa taaacaatac aatagcaaaa gtagttttgt aatgatctaa acaccaagtc
     1081 aacaattttg aaatccaatt ggtgaaacga gttaaatagt tttcaaaacc aagaataact
     1141 ctttcaaata aatttttacc ttcaatgtgt tctaattttc caaaacgaga agataaccat
     1201 ggaataattg taaaagaagc caataaggat aataaggtag aaattacaac cgtcatacag
     1261 aattgagtga taatgtcaga taccaatcct gaactcatgg caattggtaa aaacaccacc
     1321 acaattacta aggtaattga tgtaactgtt cctccaattt cggctgttcc atcatacgcg
     1381 gcacgaattc ggcttttacc catttccatg tgcctataaa tattttctaa aaccacaatg
     1441 gcatcatcca caagaatacc aacaaccaat gaaagtccta ataaactcat taagtttaag
     1501 gtatatccta aaagataaac tccaataaat gttgcaatta acgatgcagg aatagaaacc
     1561 attacaatta aagagtttcg aatactgtgt aagaagaaca acattacaat tgctactagt
     1621 attacagcaa ttaataaatc gtgaattaca gaatcggcgg cttcaagagt aaatactgta
     1681 ctatcttttg ctacttcaag ttttaaatcg ttcgctttgt attcgttttc aagatttttt
     1741 atggttgaca tcactttttc gcttactgca acagcattgg catcagattg ttttacaatt
     1801 tgtaagatga tggcactttt ttgatcaaca cgagcaattt tttcagctat tttttgtgca
     1861 tcttgaacgt cggctatgtc ttttaatcga atttgaattc cattttttga cgatactact
     1921 aaatttcgta attcatcaac ggttttatat tttccggcta aacggattaa tattttttgg
     1981 tcacgagttt gaatattacc cgttggaaaa tctaaattag aagataaaat aatttgttgc
     2041 acttcaggaa ttgataatcc ataaccttga agttttgtag catcaagatt tacctgaatt
     2101 tcacgttctt gaccaccaat aatatttact tgagcaacac ccgaaacacg agataaaact
     2161 ggagaaatct ttttgtcaat taaatcataa aaagcggctt catccatttt tccttttgct
     2221 cccaatgtaa taattggcat gtcgctaaac gagaatttag ttaacgatgg tggatcggca
     2281 tcatcgggta aatcacctaa aattgcgttt acttttcgtt gcgcgtcatt catcgaaaca
     2341 tcaacatcgg caccagatat aagtgttacc attacgaccg aaagactttc gtatgatttt
     2401 gattcaattt tcttgatgtt ttctagcgaa gcaattgcgt cttcgatttt tttggtaacg
     2461 gtattttcta tttcgcttgg cgatgcacca ggataaactg ttgagattgt gattacgtta
     2521 gtttcaaatt ttgggattag ttcataaccc aattgagtat aactgaataa tcctcctaac
     2581 gttagtattg taaaaagcac aacaattaac gtaggtcttt ttatggatat ttctgctaat
     2641 ttcattttct tgtttttata ttggatgtaa aattatttat ttaataattt ctatttttga
     2701 tccgtttgtt aagttaattt gtccagttgt aataacaaca tcgccatctt ttaaaccatc
     2761 tagaatttca actttatctc caataattct accagcggtt acttttttta acactgcaat
     2821 tccgttttca tttacaaata cttgatttcc gctaacacta ccaacaaatg cgcttcttgg
     2881 agcaatcatg attttttgtg tttgttgttt tgattcaaat tttgctgttc catacatacc
     2941 agcttttaaa tcattgtttg cattgtttga tatttcaatt tctattggaa agtttaatga
     3001 agcatctgct tttgcagcaa taaaagtgat tttgccgtta aatgttttgt caggataaac
     3061 attggttaca acatttattg gagttccaac ttttaaattt gctacttgcg actcattaac
     3121 agtaacgtta agttttagtt tagatacatt aactatttcg tataatgcgg ttgcaggcat
     3181 tgaagaaatc attgaaccta cttcgatgaa ttttttattg ataacaccat taattggagc
     3241 tttaattcta gtatcaccaa catttatatt ggcttgtgat aaattagatt ttgcattaat
     3301 cattgcaatt ctagcttggt ctaattgttg tttggttaca ccaccagttt tgtatgcgtt
     3361 ttcaaaacga ttataatcca ttaatgcgtt ttgatataca gaatttgcgt tttcggcatt
     3421 aacattaatt gcatctccac gcataatggc aagtacttgt cctacacgaa caacatcacc
     3481 ttcttttgct aatactttta caatttttcc acttttttct gccgaaaagg ttaactcttg
     3541 agtaggttca aaagttccat ttgcattgaa gtcaaaagaa atttcttctg tttttatttc
     3601 ggcagctttt actaatacgg atgaatttgt ggcagctaca atatctattt tttcctgatt
     3661 ttctttttta ttcttcatta atacaaaagc tataagcgct aatgatccta atattgcgat
     3721 tgttgttatt ataccttttt tcattttgat taattgttaa ttaaagtttt taattctcct
     3781 tttgatttta ttaattgaat ttcggctaat ttgtaatcta atattgccgt tgtatagttg
     3841 ttttgtgctt caactaatgc attttcagca tcaagtaaat cggttaaact agccaatcct
     3901 tgtatataat tattattagt atttgttaaa acttcttgag ccaacttggc attttctttt
     3961 tgattgttga tagaaactat gctgttttca atttgttttt ttgcattatc atattgtaag
     4021 tccaaggaca atttggtttc ttctaaatca acttttatgg attgtaattt attttctgcc
     4081 tgacgaactt ttgctcttgt gccaaatccg ctaaaaattg gaatgtttaa atttaaacca
     4141 acagcagcat aatcagacca atacacgccg tcggtaggtt ttttaaacca aggcatttct
     4201 ggaccttgac ctatataatt ataacttcca gttaatgaaa gagttggata atatccggct
     4261 tgaaccgatt ttttctgaaa ttctaataat tgttcgttct tttttaatag taaatattca
     4321 gttctagaag ttacatcagg ttgttctgaa agtgcatttg gtgttacttc aaatttggtt
     4381 tgaggaattg taatattagt ttcaattggc attcccatta aaaactttaa tgagttttct
     4441 tgaagcgaaa tagcgttaat tgactgttgg cgttgagtgt ttatgttaga aatacgaaca
     4501 ataattctat ctaaatctat ttttttggct aacccgttat catattgtcc tttgataatg
     4561 tctcttactt tagtagtgtt tttcaaatta ttttcaatca cgtttaagtt ttgacgcgta
     4621 acataaacct gatagtaagc gtttgcaact ctttcaataa tttgttcttc agttagttga
     4681 gcgttgattt gataaaactc tcttgtgctt ttagctgctt ttaaaccaat aaataccgat
     4741 tggtcgtaca aagcttgttg aagtgtaact ccagcaccag atgtccattt ttgtccaaat
     4801 gtagcttgaa tggttgaacc tggagcacca aaactcgatc catcaataac actggtttgt
     4861 aaaatagggt tgtgatttaa atttccgctg gcagaaattt gtggtaaagc tcgtgagcgt
     4921 acttcttgaa tttgatattc gctattttct acatctaatt ttgccttttt tgcatcagct
     4981 ttattttgta atgcataagt aatggcgtct tttaaggtta gcggtttttc ttcttgtgct
     5041 atcgagagag caaatgttaa tagaaaaacg attattaatt tggttttcat tttgattttt
     5101 ataattgatt tttaagtaat tgttctaatt ttattattcc ttttgaagtt ccaattcctc
     5161 taatatggta ttctaaaaat tctagttgaa ctttttcggg attgaatttc tcaattggaa
     5221 aataatcgtt gtcttttaaa ttagatgacc cataaaaata tattcgactt ataattgaaa
     5281 tatcaatatc attacggaat aaaccatctt taataccgcg atttatgttt ttgtttatgc
     5341 aggtttctaa tttttctaaa tgcttttttt taagcctatc atgtattttt ggataatatt
     5401 tttgaaattg ataaatggta gattgattac ctgctttaaa gtaatctgaa atatagtttt
     5461 taacattaaa aagttcttct atcggatttt tatttcctcg atatatttca tcaatacctt
     5521 tagtaatttc atcaaataag taagaagctg tttcttttac taattcagtt ttattagaaa
     5581 aatgttgata aattgttttt tttgaaattc ccatttcatt tgcaatatca tccattgtca
     5641 cgcttttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg ttttttttga
     5761 aattcccatt tcatttgcaa tatcatccat tgtcacgctt ttaaacccaa gtgacataaa
     5821 cattttgctt gctttttcta aaattttatc tttcattttg tcaaaatttc gtgcaaatat
     5881 agattggaaa cttttaaaac aaaaaaagtt tccatagttt ttacaataat ttaacatttg
     5941 atttagaagt gaaattgaca ataccgtaat aaaaacttat tttagccgaa aatattttat
     6001 gcatcctatt aagcattatc aagagctaat ttcaaatcat tttcatcaaa ttaatttggt
     6061 taatgaacct acaaatcttt accaaccaat tcagtatatt ttatctttag gtggaaaaag
     6121 attacgacca gttttaacat taatggcaac cgaagttttt gatgtagatt gtaaaaaagc
     6181 attggctgca gcaacggctg ttgaggtttt tcataatttt tcgttaattc atgatgatat
     6241 tatggatgat gctcctttgc gtagaggaaa agaaacggta catgaaaaat gggatattaa
     6301 taccggaatt ctttctggtg atgccatgtt gattttggcc taccaatatt ttgaagaata
     6361 cgaacctaaa gtttttagag acttagccaa actttttagc aaaacagcac ttgaggtttg
     6421 tgaaggtcaa caatatgatg ttgattttga aactcgcgac gatgttacca ttgctcaata
     6481 tttaaaaatg attgaatata aaacggcggt tttggttggt gcagctatga aaatgggagc
     6541 aattgtggct gaaacatccg aagaaaatgc taatttaatt tacaattttg gacttaattt
     6601 aggaattgca tttcaattgc aagacgatta tttagatgct tttggaaatc cagaaacttt
     6661 tggaaaacaa gtaggcggcg atattattga gaacaagaag acctatttat acttaaaagc
     6721 tcttgagttt tcaaatgact ctgaaaaaga acaattgttg catttgtttt ctattcagcc
     6781 taatgataat accgataaaa ttaattcggt aaaagcaatt tttaatgcaa caggctctag
     6841 cgacgcgact caaaaagcca ttcaagagtt tactttcaaa gcctttgaaa ccttagataa
     6901 aatggatatt agcgatgata aaaaaatact gttaagaaca tttggtgaaa agttaatgaa
     6961 tcgtgatgtc tagttttgta gcgccaatat cgacagactt tttgcttttg gaggccgaaa
     7021 aagaaaatct ttatcaaaaa ctgattgagc aactcaacaa agattttaat ttggctaatg
     7081 aagcaatcga ttttccaaaa agcacaactc ctaatgaact taaagtacag ctccacgaaa
     7141 aaatttatcg tttaattcaa tacaagtttg ccgaatatct gaacttgctt tatatcatcg
     7201 atgtatccga aagcgaagtc aaaaaactca atggtgagga cttagtcgaa ctctcaaaac
     7261 aagtagcttt tctaatccta aaaagagaat ggcaaaaagt ttggtttcgc aataagtatt
     7321 aaaaataaac cctcacaaac ggcataaaag catcgccata cacatttctg tctttatcaa
     7381 aaagaacgtt atatcgtgcg ccaatagtaa cgttttgtgt tctatatcct gcaccaagaa
     7441 acaagcctgt attccaaaaa ttttctttta aattaatgtt ttcatagttc acatttacgc
     7501 gtagttgttc taattcaacc gaaagttgaa tttcttcaat gggattaacc aatccaataa
     7561 tacttccgcc ataagtgtga ctgtcaaata agtttttttg tttcaaataa ctgtattgca
     7621 atccaacacc agcagaaacg tattgattga agttgtatat tgcacttgga gccacagcaa
     7681 tattagtaaa accgttgcca ataccgagac ctaatccgcc accaaattga acatgttccc
     7741 aaaactcatt tttggttttt aaagaatcat ttttttgaga ataaatgctt gatataaaag
     7801 agaaaaaaaa gagtgctaca acaaacaaag ttctttttaa gttcagaata attttcatcg
     7861 gtactatttt gaaaatttta gtgttataaa tgtactaaaa tcgttgataa ttattgcaaa
     7921 tatcatactt ttgcacttca tttactaaca gtaatatttt ttagcatcaa aacatggata
     7981 ggttttcctt tttaaacgca gcacataccg aattttttgc cgatttatac gaacaatatc
     8041 ttcagaatcc cgattctgtt gaacctagtt ggagagcttt ctttcaaggg tttgattttg
     8101 ggatggaaac atacaacgaa gaacaatttt ctaaaaatac ttctgaaaat caagattgta
     8161 gtttagtttc ggacaaacta caaaaagaat ttaatgtatt aaagctaatt gatgcctaca
     8221 gaactcgtgg tcatcttttt acagaaacca atcctgttag agaacgaaga acttatgttc
     8281 caactttaga tttggaaaat tttgggcttt ctacttctga tttagatacc gtttttgatg
     8341 ccgctaaaat tttaggacat caaccacaaa ctttaaaaga aatccttacg catttaaaaa
     8401 atgtgtattg taagcatatt ggtgttgaat atatgtacat gcgtaggcca gaagtaattc
     8461 agtggatgca agaaaaatta aacatcaatg acaatcttcc caactttact gtagatcaaa
     8521 aaaaacacat tttgggaaaa ttaaacgaag ccgtttcttt tgaaaacttt ttgcacacca
     8581 aatatgttgg tcaaaaacgg ttttcgcttg aaggtggaga aagtattatt ccaggacttg
     8641 atgccgttat tgaagcggca gccgaaaaag gagtagagca ttttgtaatg ggaatggcgc
     8701 acagaggaag attaaatatc ttagcgaata tttttggaaa agctactcaa gatattttct
     8761 ctgaatttga tggaaaagat tatgatcaag aatattttga tggtgatgta aaataccatt
     8821 tgggactaac atctgaaaga gtaactactt caggtaaaaa aataaatatc aatttagcac
     8881 caaacccttc acatcttgaa actgttggtg ccgttattga aggaataacg agagcaaaac
     8941 aagacaaata tttcccagac gatttctcca aagtactacc aattgccgtt cacggtgatg
     9001 ctgctgttgc tggacaaggt attgtttatg aaatcgttca aatgtcacaa ttagatggtt
     9061 acaaaactgg tggaaccatt catattgtta tcaataacca agtaggcttt acaaccaatt
     9121 atttggatgc tcgttcgtca acgtattgta ctgatattgc aaaagtaacc ttgtcgccag
     9181 tgctacacgt aaatgctgat gataccgaag cagtagttca tgcgatgtta ttcgccctag
     9241 atttcagaat ggaatttggt cgcgatgtat ttattgactt attagggtat agaaaatatg
     9301 gtcataacga aggtgatgaa ccacgattta cacagccgct tttatataaa atcattgcaa
     9361 aacataaaaa tccaagagat atttatgcag caaaattaat agaagacgga atcattgatg
     9421 ccaactatat ttctcaaatt gaaaacacat acaaagagaa attagatgaa aatcttgaag
     9481 cttctcgcaa aaaaaactta acagtcatca agccgtttat gcaagatgaa tggcaaggtt
     9541 tcaaacaagt ttctgatgat gctatgcgta aaaaagtaga tactactttt gatagaaaaa
     9601 aattagacca aattatcgaa actgtttcta gtttgccaaa agacaagaag tttatcaata
     9661 aaatttctaa aattgttaca gacagaaaaa ctcaatacga caatgatgtg ttggattggg
     9721 gaacagcaga aaccttagcc tacggaacat tattaaccga agggtttgat gttagaatct
     9781 ctggtcaaga tgtggaacgc ggtacttttt cgcatcgtca tgccgtggtt aaagtagaag
     9841 atagcgaaga agaagtagtt ttattaaaca ctttgaaaga caaaaaagga aagtttaacg
     9901 tattcaattc gttgctatca gaatatggcg ttttaggatt cgattatggg tatgccttag
     9961 ccaatccgaa tgcgttgact atttgggaag ctcaatttgg agatttttct aatggttgtc
    10021 aaattatgat tgaccaatac atttcttgtg gtgaagataa atggaacaac caaaacggta
    10081 ttgttttatt attgcctcac ggatatgaag gtcaaggtgc agaacattca tcagctagaa
    10141 tggaacgtta tttacaacta tgcgcacgtc ataatatgta tgttgctaat tgttcaacgc
    10201 cagccaattt cttccacttg ctaagaagac aaatgaaaac gaatttccgt aaaccattaa
    10261 ttcatttctc tccaaaaagt ttattgcgtc acccattatg tgtttctaag gttgatgatt
    10321 tagtaaaagg tcaatttcaa gaaacaattg atgacacttc ggtagataag agtaaagtaa
    10381 aaacactggt tttctgtacg ggtaaattct attatgatat acttgccgaa agagaaaatt
    10441 taggcagaaa tgatgtagct ttggtacgaa tagaacaatt gtttccattg ccagttgacc
    10501 aattaaaaga tattattgct agttatccaa atgcagatga ttacgtttgg gcacaagagg
    10561 aaccaaaaaa catgggtgct tacagtttca tgttgatgaa cttcgactta gtaaaatgga
    10621 gattagcttc tttaaaagcg tatgccgcac cagcagcagg aagtcatact agagatagac
    10681 gtcgtcatgc ggatgcaata cgaatggttt ttgataaaaa tttatttaga tagaacatgc
    10741 caactgtttt ttacattaat ggatatagat ttttctttta tagtaatgaa catttaccaa
    10801 agcatataca tattgaaaag gctgaaaaaa cagcaaaatt cagtttagaa agtattgaat
    10861 taattaagtc tttcgggttt aatgcatctg aattaaaaga aattcgtaat ttagttgaag
    10921 aaaatcaaga acttttaata caaaattggg atgagttttt taacaattaa taaatcgaaa
    10981 aaagcaatta gtattgcatt tgaaaattct aaaatgatag tttttttaga agatggaaga
    11041 gaattgtcaa ttcctttaga atggtttcca agattaagaa aagcatctga agaacaactt
    11101 aataaatgga ggttaattgg gaaaggagaa ggaattcact gggaagaaat tgatgaagat
    11161 atttcaattg aaaatttgtt agaataaaat aaacaatata tataaaatta taaaaggatg
    11221 attttagaaa tgaaagtgcc ttcaccaggc gagtcgataa aagaagttga aatagcaact
    11281 tggttagtaa aagatggcga ttatgtagaa aaagaccaag caattgctga ggttgattcg
    11341 gataaagcaa cgttagaatt gcctgcagaa gaaagtggga ttattacact taaagccgaa
    11401 gaaggtgatg cagttgccgt tggtgctgta gtttgtttaa tcgatactag tgctgcaaag
    11461 ccagcaggaa atgctccagc agctgaagca aaaccagccg ccgaaacacc aaaaaaagaa
    11521 gaagtaaaac cagcacctgt tgctgagaaa acatacgctt ctcaaacgcc ttcgccagca
    11581 gctagaaaaa tattagatga aaaaaacatt caacctagtg atattgttgg aacaggaaaa
    11641 gcaggaagaa taaccaaaga agatgcagaa aatgctgtgc catctatggg aactccaact
    11701 ggtggtgctc gtggttctga aaaaacaaaa ctttcaatgc ttcgtcgtaa agtagctgaa
    11761 agattagtat cagctaaaaa tgaaacagca atgttaacta cgtttaacga agtggacatg
    11821 actgctattt atgagttgag aaatcaatac aaagaagaat ttaaaaacaa acacggcgta
    11881 ggtttaggct ttatgtcgtt ctttactaaa gctgttactc gtgcgttaca actgtatcca
    11941 gatgtaaact cgatgattga tggtgattat aaaattgctt acgatttttg cgatatttcc
    12001 gttgccgttt caggtccaaa aggcttgatg gttccggtaa tgcgtagtgc agaaaaccta
    12061 tcattccgtg gcgtagaagc ggaaatcaaa cgtttagcaa tccgtgctcg tgacggacaa
    12121 attacggtgg atgaaatgac gggtggaacg tttaccattt ctaatggtgg tgtttttgga
    12181 agtatgttga gcacgccaat tattaatcct ccacaaagtg gtattcttgg aatgcacaat
    12241 gtagtagaac gtccaatcgt taaaaatggt caaatcgtaa ttgcaccggt aatgtttgta
    12301 gcattgtctt atgaccacag aattattgat ggtcgtgagt cggttggttt cttagtcgct
    12361 gttaaagaag ctttagaaaa cccaacagaa ttattaatgg ataataatcc taaaaaagcg
    12421 ttggaattat aatataaatt agattttaga caaaaagaaa acccgatttg aaacatttca
    12481 aatcgggttt tatattactt ttattttagt aattaatatc aaatgaacca tttgtaacgg
    12541 ttaaagtact gccagtagct gaatcttgta acgtaccgga aaaagtgcct tttaaatttt
    12601 ttgaactgtc atttacagtt acatttgtgt taaaagaggc tgtcctataa tactcattgt
    12661 cattttctgt ataacttaaa tagtaaatta catctgtacc agttactcct ttttctaagt
    12721 taatagaaat cgtttcagta tctgtatcat tagtagctaa aaccattaaa tcagtatatt
    12781 catctggagt tccagcttca aatatctctt ctgttacaga aatagtgtta aacgttttta
    12841 cttgtccacc aaccgtcata gatattcttt ttgacgtact tgaatttgta tcacttttag
    12901 aacaagcact aaatgttagt gataccgcaa atagataaag aaataatttt ttcattttag
    12961 tttaaattta aatacaaaaa tacgacataa attctttgtt tttattacta acgcaatatt
    13021 ttttcataac gcaaaacaat actcttaggt tttgttttaa tatcatgttt aatgaggtta
    13081 gtgatagaat tctaaaaaaa acttttttgt ttttgacatc catgtacaat tttaaaatat
    13141 aaaaactagc gttttcctct tttaaaatca ttcgaagtaa cgcatattga gcaacatttt
    13201 ttctcca
//
LOCUS       DAOOBH010000062        13163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_6085_length_13163_cov_6.682865, whole genome shotgun sequence.
ACCESSION   DAOOBH010000062 DAOOBH010000000
VERSION     DAOOBH010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6085_length_13163_cov_6.682865"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK218_07410"
     CDS             <1..531
                     /locus_tag="PK218_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HPW98370.1"
                     /translation="ASGCFIHEKHHSSDLPRFAGWWAQKKEKRFLMEPKFDPTIGADG
                     WQVSNLPILSLAPYLASVEMFAKVGMEKLIAKRNQITAYLEFILHEIDKEIDGTEFEI
                     ITPQNQEERGCQLSVFLHGQGKDLFHYLMKNGVITDWREPNVIRLAPAPFYCSYEDMY
                     KFGQILKTGILSLSKS"
     gene            531..1619
                     /locus_tag="PK218_07415"
     CDS             531..1619
                     /locus_tag="PK218_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HPW98371.1"
                     /translation="MTKEQIIQNFDPSQPGLADATIFGLPFSAEQSEIIIIPVPWEVT
                     VSYGSGASEGPNAILDASFQVDLNHQEFPELWKLGMYFAEIPDSWSTNSEKYKKLAQP
                     IIEALEAGENIETFPALLEDLHKINKVCRELHTEVKEKVLYWMNQGKKVVLLGGDHST
                     PLGYYEALATKHDNFGILHLDAHMDLRIAYEGFTYSHASIMYNALQIPQISKIVQVGI
                     RDFCEQEVGVVKNENGRVLIHTDADLKAENFEGKTWAKQCDEIIASLPQKVCISFDID
                     GMLPWYSPNTGTPVPGGFSFEQATYLFNKLASSGKEIIGFDLVEVAPGENDDWDGNVG
                     ARMLFHMCGVLAKNNGMKTGNRITFQRK"
     gene            complement(1659..2057)
                     /locus_tag="PK218_07420"
     CDS             complement(1659..2057)
                     /locus_tag="PK218_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98372.1"
                     /translation="MQTFFLFLAKWIVVFFGIFITITGFVMLFQPAKARSILRKAGST
                     NFINYTEITLRLIPAFGLIYYAEVSKYTLFFQLFGWCMLLTSLVLYFVPRNLHHKFSN
                     KSAAILKPSYFQLIAPFAFLLGSFIIYCVV"
     gene            2200..2601
                     /locus_tag="PK218_07425"
     CDS             2200..2601
                     /locus_tag="PK218_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HPW98373.1"
                     /translation="MKTIYTIIVSILILSCKNTNDKVVVIDEKQETIDSMKMQIEKHR
                     IIDSMREVELNNAKKREVLVLKESNTTNKTTERKKWSGAAKGAVIGAGVGAITGAIVS
                     EKKGEGAVIGGLAGAGVGAGTGAIIDDKKKK"
     gene            2722..5109
                     /locus_tag="PK218_07430"
     CDS             2722..5109
                     /locus_tag="PK218_07430"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPW98374.1"
                     /translation="MKILKKTAILLLAIMIITLLGIVVFLQFKKPNYETEIELIQLSK
                     PTTTYFDTYGIPHIYAENEIDAQRVLGYVHAQERLWQMELMRRIAPGKLSEIFGSVMV
                     KNDKFFVTSGIEENSEITIANLDKNTETYKLTQAYLDGINQYIAEGTMPVEFTLLGIS
                     PEKFTLKDVYNIFGFMSFSFAMAQKTDPLLTDIRDNLGMEYLADFGIHGELGKKQLKS
                     FKGKTSEFAEISKSITAILEKSPVPAFIGSNSWVIGANKTKNRKVIFANDPHIMFAQP
                     GTWFEAHIVTPKHEMYGYYLAGTPFPLLGHNREYAYGLTMFENDDLDLFEEKENPENV
                     NEYLTKNGYKKYYFLRKTIKVKDSSTIVLNVKSSLHGPIISNVLAGANKEKSIAMSWI
                     YTQQKNQILEAVYGLSHAMNLDDFHAKIELIHAPGLNIMYGDAKDNIAWITSGKLYKL
                     DKTVNPNFILNGSNGIDDQKEFLPFSENPFAVNPPWNYVYSSNNQVEKINGYLYPGYY
                     LPKDRALRIDTLLSVKSDWSRKDVEQMILDNTSSTASQIVNNWILTLNQNQFSETEKV
                     AIDELKKWSGSSNLNDIAPTIYNKWIYFYLKNTFEDELGEEKFKLLLGTHIMKQMFGS
                     QSQKEQSIWWDDITTKNKKETRSEIMTLAFKEAIKNLETNFGSDVKLWNWKKAHTLEF
                     QHPLGQVKLLKGFFNVGPFEVPGANEVINNLMYFYSDEKINQVKAGPSTRRIIDFSDV
                     ENSVSILPSGNSGNPMSKHYNDQSEMYVNGQFRKMLLNKKEIEKCSTKMVFSPKK"
     gene            5170..6519
                     /locus_tag="PK218_07435"
     CDS             5170..6519
                     /locus_tag="PK218_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW98375.1"
                     /translation="MQTPQKIAVVGSGLVGTLLAIYLKKLGHKVHVFDRSPDIRTVEF
                     SGRSINLVMSNRGWKTLEDIGLSDEIKKIGIPVDKRGIHLQDGSYTTQFYGKEGEAIY
                     SLSRGILNRRMIDLAEEAGVEFFFNHKVWDVSLSTATLHIGETERGEWTDLKYDKVFG
                     ADGAFSRVRHRMQRQSMFDYSQEFMKIGYKELHIPANSDGSHKIDKNSLHIWPRGNFM
                     LMGLANLDGSFTCTLFMPFEGENSFEQLKDEKTLVDFFAKYFPDTKEVIPDLVEDFFK
                     NPKSYLVMMKCFPWSHNDKVALIGDSAHAIVPFYGHGMNAGFEDITILNEMIAKYGDD
                     WEKIFQEYEQSRKPNADAIAELSFRNFMEMSSKTADENFLLQKKIEKWFSDKHPDKWM
                     PLYSRVTFSLQPYSEALAIGEFQNKIMEQVMQFPNIQHNWNSDEVEKLILENLKYKI"
     gene            complement(6528..6887)
                     /locus_tag="PK218_07440"
     CDS             complement(6528..6887)
                     /locus_tag="PK218_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW98376.1"
                     /translation="MKKYFIQKSPFVVPTIDGKIIKEHHGKVATNNSEISIAHMMAPP
                     KWSEPFQTPEFDEYTFIISGKKQFIIDGETVILEAGQSIKIEKNTRVQYSNPFDEPCE
                     YIAICNPAFDFEKVNRE"
     gene            complement(6890..7258)
                     /locus_tag="PK218_07445"
     CDS             complement(6890..7258)
                     /locus_tag="PK218_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPW98377.1"
                     /translation="MKGYITIGLLILSNIFMTLAWYGHLKFKELKWFENAGLLTIVLI
                     SWGLAFFEYCFQVPANKIGYEGNNGPFSLLQLKVIQEVITLVVFVGFSLFFFKNETFK
                     WNHFVGFCFLILAVYFIFKK"
     gene            complement(7312..8844)
                     /locus_tag="PK218_07450"
     CDS             complement(7312..8844)
                     /locus_tag="PK218_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPW98378.1"
                     /translation="MSKETEFSEYINTNYNTKGENIILGGAILDGNALSDTLVKIPLK
                     TLNRHGLIAGATGTGKTKTIQVFSEQLSAQGIPVLMMDIKGDFSGIAAEGEEKSFITE
                     RHAKINLPYTIQKFPVELMSLSSQNGVRLRATVSEFGPVLFSRILDLNDTQSGVVSVI
                     FKFCDDKQMPLLDLKDIKKVINYITEEGKSEIEEHYGKISTSTTGIILRKIIELEQQG
                     GDIFFGEKSFEIDDLMRIDENGKGYVNIIRLTDIQDKPKLFSTFMLSLLAEIYQQMPE
                     KGDAERPELVIFIDEAHLIFNEASKTLLDQIETIVKLIRSKGIGVYFVTQNPMDVPAS
                     VLAQLGLKIQHALRAFTAKDRQAIKQTAENYPITKYYKTDEVLTSLGIGEAFVTALNE
                     KGIPTPLVATMLRAPMSRMDILSESEIDAINEKSKLVKKYNEEIDRESAYEILTKKID
                     EANQKASEEAAKAEEIKTTTKRKANEPSTAEVIGKSVTKVVTSATFIRGVFGILSKMF
                     KK"
     gene            9212..10033
                     /locus_tag="PK218_07455"
     CDS             9212..10033
                     /locus_tag="PK218_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPW98379.1"
                     /translation="MANVKKESSSNGGNIFSGLVIVGCVLVGILVWKFIMGDGSNFEG
                     GVNTGHPLPGNYLAMVYKGGAVVPVLMGLFLMVIVFSFERFFVISKAGGKGNLDTFMA
                     NVQASIKQGNIDDAIAACDKQQGSVANAIKAALVKYQDVKKEGFNSEDAAETIQKEIE
                     EATSLEMPMLEKNMTILSTLVSLGTLAGLLGTVTGMIKAFGALASAGTPDQAALANGI
                     SEALINTATGISTSALAIVTYNFFTSKIDTLTYSIDEAGATIVNTYRKFRGSLKS"
     gene            10076..10714
                     /locus_tag="PK218_07460"
     CDS             10076..10714
                     /locus_tag="PK218_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPW98380.1"
                     /translation="MAKVKMSKKSTSIDMTAMCDVAFLLLTFFILTATAKTPEPLPVE
                     TPNSTVQTKLPETGLVTITIGKSDGKNQVFFGMKDRDVRVGALEYMSEKYGVTFTDKQ
                     KAQFELVEEFGVPIEQLGSILDMKAADRAKVGVQSGIPLDSIGENKQQLQDWIQYARK
                     ANIDRGATKELDFAIKGDAKEEYPQIKQIMDILQNQKINNFNLVTGLRGKDY"
     gene            10733..11290
                     /locus_tag="PK218_07465"
     CDS             10733..11290
                     /locus_tag="PK218_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPW98381.1"
                     /translation="MAELNTGDGGGKKGGGKVRSKKSNSKVDLTAMVDLAFLLITFFM
                     LTTSLSKPQSMDLGLPDKDEKVDTNIKVDQVRTMTIILGENNKIKWYHGLLESPEPNG
                     KPTDATYGKDGIRKEILKRVKSIPIATGNPKKGMIVIIKPTKKSTYRNLVDILDEMAI
                     ANVPTYAIVNDITPEEMKLVDEMNK"
     gene            11322..12113
                     /locus_tag="PK218_07470"
     CDS             11322..12113
                     /locus_tag="PK218_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW98382.1"
                     /translation="MKLDLFKKKWIDIVFEGRNKAYGAYELRQENPKTTIRSFIIGAF
                     IFGLAVGAPLIASLIPDSQEKDTSLDKKIVTIKLPPKKEAPKNLPPPPPPPPKVDQVK
                     FVKPVVAKTEEIVEEPPKIVEIKEKKLGAETIKGDPDAPLTIEPVGNGPSKVVEEDNS
                     IYNTAGIQVQPEFPGGMDKFYKFIGKNYTPPDEEGLKGKVFVTFVVEKDGTLTDIKVL
                     RDIGYGTGKEAIRVLKACPRWNPGEQNGKKVRVQYSLPITIQSAY"
     gene            12343..>13163
                     /locus_tag="PK218_07475"
     CDS             12343..>13163
                     /locus_tag="PK218_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW98383.1"
                     /translation="MNKKHIFFIVLSFVFVIFFACKKNNQDEETIIKGKATLLVDESI
                     FPIVEDQQAVFETQYDAKLSLVRKSEAEIINSLLNDTLKIAVIPRKLTDAEDKAFSNK
                     KIFPKQTIFAKDAVVFITNKMSKDSLIALEDIVKFMKGEDVKGIKGLVFDNPNSSTVR
                     LLSEKAGIKVTPQKNIFSFATNNEVIKYVSQNEGMIGVVGVNWLFQPPLDMQPTVDKV
                     SIMSVKSDEKFGFVYPSQENIGNGKYPLARHLYIINCQGYSGLGMGFASFLGGERG"
BASE COUNT         4811 a         1900 c         2290 g         4162 t
ORIGIN      
        1 gcttcgggtt gttttattca tgaaaaacat catagttcag atttaccaag atttgcgggt
       61 tggtgggcac aaaaaaaaga aaaacggttt ttgatggagc caaaatttga cccaacaatt
      121 ggagcagacg gttggcaagt ttccaattta cccatcctgt cattagcacc ctatttagct
      181 tcggttgaaa tgtttgccaa agttgggatg gaaaaattaa ttgctaaaag aaatcaaatc
      241 acagcttacc ttgaatttat tcttcatgaa attgataaag aaattgacgg aacagaattt
      301 gaaatcatca caccgcaaaa ccaagaggaa agaggttgtc aattatcggt ttttcttcat
      361 ggtcaaggaa aagatttatt tcattattta atgaaaaatg gagttattac cgattggcgt
      421 gaaccaaatg ttattcgttt agctccagca cctttttatt gttcttatga agacatgtac
      481 aaatttggtc aaatattaaa aactggaatt ttaagtttaa gtaaatcata atgacaaaag
      541 aacaaatcat acaaaacttc gatccatcac aacctggttt agcagatgct accatttttg
      601 gattaccttt ttctgccgaa caatctgaaa tcatcattat tccagtgcct tgggaagtta
      661 cagtaagtta tggttctggt gcttctgaag gtccaaatgc tattcttgat gcatcatttc
      721 aagtagattt aaaccatcaa gagtttcctg aattatggaa attaggaatg tattttgccg
      781 aaattccaga ttcttggtca accaattctg agaaatataa aaaattggca cagccaataa
      841 tagaagcatt ggaagcaggt gaaaatatag aaacttttcc agcattattg gaagatttac
      901 acaaaatcaa caaagtttgt cgcgaattac ataccgaagt aaaagaaaaa gttttgtatt
      961 ggatgaatca aggtaaaaaa gtagttttac ttggtggcga tcattctaca ccgttaggat
     1021 attatgaagc attagcaaca aagcacgata attttggaat tcttcatctc gatgcacata
     1081 tggatttgcg aattgcttat gaaggcttta cctattcgca tgcatcaata atgtataatg
     1141 cgttgcaaat ccctcaaata tcaaaaatcg ttcaagttgg aattcgtgat ttttgcgagc
     1201 aagaagttgg agttgttaaa aatgaaaacg gcagggtttt aatacatacg gatgctgatt
     1261 taaaagctga aaactttgaa ggaaaaactt gggcaaaaca atgtgatgaa attattgctt
     1321 cattacctca aaaagtatgc attagttttg atattgatgg gatgcttcct tggtattctc
     1381 caaatacagg aacgccagtt ccaggaggtt tctcgtttga acaagcaacc tatttgttta
     1441 acaaattagc atcgagtggc aaagaaatca taggttttga tttggtggaa gtggcacctg
     1501 gtgaaaatga tgattgggat ggaaatgttg gtgccagaat gttgtttcac atgtgtggtg
     1561 ttttggccaa gaacaacgga atgaaaacag gaaaccgaat tacatttcaa agaaaataaa
     1621 atcacagcct ctttttttga agaggttttt ttatgcgatt atacaacaca ataaataata
     1681 aaactaccta gtaaaaaagc aaatggagca attagttgaa aataagaagg ttttaaaata
     1741 gcagcacttt tatttgaaaa tttatgatga aggtttcttg gaacaaaata aagcactaat
     1801 gatgttagca acatacacca accaaataat tgaaaaaaca aagtatattt tgaaacctct
     1861 gcatagtaaa ttaaaccaaa tgctggaatt aacctcaaag tgatttctgt gtaattgatg
     1921 aagttagtgc ttccagcttt tcttaaaatt gatcttgctt ttgctggttg aaataacatt
     1981 acaaagcctg ttattgtaat aaatattcca aaaaacacta ctatccattt cgccaaaaac
     2041 aaaaaaaaag tttgcatagt ttagtgtatt tagttgtaaa aataactttt actattgaaa
     2101 agtaaattaa attatataat tttttcaaaa aattatgtaa cattaaattt aatcaactaa
     2161 cataaatttg taaggtaatt caaaagcaat aaagcaatca tgaaaacgat ttatacaata
     2221 atagtttcaa tactaatttt atcttgcaaa aacacaaatg ataaagttgt tgttattgat
     2281 gaaaaacagg aaactattga ttcaatgaaa atgcaaattg aaaaacacag aatcattgat
     2341 tctatgagag aagttgaatt gaataatgcc aaaaaaagag aggtattggt attaaaagag
     2401 tcaaacacaa ccaataaaac aacagaaagg aaaaaatgga gtggtgctgc taaaggtgct
     2461 gttattggag ctggagttgg agctataaca ggagcaattg taagtgagaa aaaaggagaa
     2521 ggtgctgtaa ttggaggatt ggcaggtgca ggagtaggcg caggaacagg tgcaattatt
     2581 gatgataaaa agaagaaata ataaaattca tgttttgtta taaaaggagt taatttcagg
     2641 gatggaatta attccttttt atttttacag ttctgctatt ctcaaaattc atttcattaa
     2701 atttgttgaa atacccaaaa aatgaaaatc ctaaagaaaa ctgccattct actccttgca
     2761 ataatgatta taacacttct aggaattgtt gtatttctac agtttaaaaa accaaattac
     2821 gaaaccgaaa ttgagttaat tcaattatcc aaaccaacga ctacatattt tgacacatac
     2881 ggaattccac atatttatgc tgaaaatgag attgacgcac aacgagtttt aggatatgtt
     2941 catgctcaag aacgtttatg gcaaatggaa ttaatgcgaa gaattgcacc aggaaaatta
     3001 tccgaaattt tcggttcagt tatggttaag aatgataagt tttttgtcac ttcaggaatt
     3061 gaagaaaatt cagaaataac cattgccaat ttggataaaa atactgaaac ctacaaattg
     3121 acacaagcct atctagacgg aataaatcaa tacattgcag agggaacaat gccggtcgaa
     3181 tttacgctat taggtataag tcccgaaaaa tttacgctaa aagatgttta taatattttt
     3241 ggttttatgt cgtttagttt tgcaatggca caaaaaacag atccactatt aaccgatatt
     3301 cgtgataatc ttggaatgga atatctggca gattttggaa ttcatggcga attaggaaaa
     3361 aaacaactaa aaagctttaa agggaaaact tctgaatttg ctgagatttc taaatcgatt
     3421 actgccatat tagaaaaatc acctgtgcca gcttttatcg gtagcaatag ttgggttata
     3481 ggagccaata aaactaaaaa tagaaaagta attttcgcaa acgatcctca tatcatgttt
     3541 gcccagccag gaacttggtt tgaagcacat atagtaacac caaaacacga aatgtatggt
     3601 tattatttag caggaacacc atttcctttg ctaggacaca atcgcgaata tgcctatgga
     3661 ttgactatgt ttgaaaacga tgatttggat ttgtttgaag aaaaagaaaa tccagaaaac
     3721 gttaatgaat atctaacaaa aaacggttat aaaaagtatt attttctacg aaaaacaatc
     3781 aaagtaaaag acagctcgac tattgtttta aatgtaaaat catccctgca tggaccaata
     3841 atttccaatg ttttagctgg cgcaaataaa gaaaaatcta tcgcaatgtc ttggatttat
     3901 acacaacaaa aaaaccagat ccttgaagct gtatatggat taagtcacgc catgaattta
     3961 gatgattttc atgctaaaat tgaacttatt catgcaccag gattaaatat tatgtatggt
     4021 gatgccaaag acaatatagc atggattacc tccggaaaat tatacaaatt ggataaaacg
     4081 gttaatccaa atttcattct aaatggaagc aatggaattg atgatcaaaa agagttttta
     4141 ccgttttccg aaaatccatt tgcagtaaat cctccttgga attatgttta ttcatctaac
     4201 aatcaagttg agaaaattaa tggttattta tatccaggat attatttacc aaaagacaga
     4261 gcattaagaa tagatacttt gttaagtgtg aaaagcgatt ggtcaagaaa agatgtagag
     4321 caaatgattt tagacaatac ttcgtcaaca gcttcacaaa tagtaaataa ttggatttta
     4381 actttaaatc aaaatcaatt ttccgaaaca gaaaaagttg ctattgatga attaaaaaaa
     4441 tggagtggta gcagtaattt gaatgatatt gctccaacca tatacaacaa atggatttat
     4501 ttttatttaa aaaacacatt tgaagatgaa ttaggtgagg aaaaattcaa attattatta
     4561 ggaacacata tcatgaaaca aatgtttggt tctcaaagtc aaaaggaaca atccatttgg
     4621 tgggatgata ttactacaaa aaacaaaaaa gaaacgcgtt ccgagattat gactttagct
     4681 tttaaagaag ctatcaaaaa cttggagacg aattttggtt cggatgtaaa attatggaat
     4741 tggaaaaaag cacatacttt agaatttcaa catccattgg gacaagtaaa attattaaaa
     4801 ggatttttca atgtcgggcc atttgaagtg cctggtgcca acgaagtgat taataattta
     4861 atgtatttct atagtgatga gaaaattaat caagtaaaag caggaccatc aacaagacgc
     4921 attattgatt tttcagatgt tgaaaatagt gttagtattc tgccttctgg aaattctgga
     4981 aatccaatga gtaagcacta taatgaccag tcggaaatgt atgtaaacgg gcaatttaga
     5041 aaaatgcttt tgaataaaaa agaaatcgaa aaatgctcga caaaaatggt ttttagtcca
     5101 aaaaaataag tcatttttct aattttcgta tctaaaacca taaatttgca aaaccgttat
     5161 taaaaataaa tgcaaacgcc acaaaaaatt gcagtagttg gttccggact tgttggaaca
     5221 ttattagcaa tatatctcaa aaaattaggt cataaagttc atgttttcga tcgtagtcca
     5281 gatattcgaa cggtagagtt ttcgggtcgt tcgattaact tggtgatgtc aaaccgagga
     5341 tggaaaacgt tagaagatat tggattatcc gatgaaatca aaaaaatagg aatcccagtc
     5401 gataaacgtg gaatccattt gcaagacgga agctatacga cacaatttta tggcaaagaa
     5461 ggtgaagcaa tttattcgct ttcaagaggt attttaaacc gaagaatgat tgatttagcc
     5521 gaagaagccg gtgtagaatt tttctttaat cataaagttt gggatgtttc tttatccaca
     5581 gcaacattgc atattggcga aactgagcgt ggcgaatgga cagatttaaa atacgataaa
     5641 gtttttgggg ctgatggtgc tttttctaga gtacgacata gaatgcaacg tcaatcgatg
     5701 tttgattatt cgcaagaatt tatgaaaatt ggctacaaag aattgcatat tccggccaat
     5761 agtgacggtt cgcataaaat tgataaaaac tcattacaca tttggccaag aggtaatttt
     5821 atgttgatgg gtttagccaa tcttgatgga agtttcacct gcaccttgtt catgcctttt
     5881 gaaggagaga actcttttga gcaactaaaa gacgaaaaga cccttgttga tttttttgca
     5941 aaatattttc cagacaccaa agaagtaatt cccgatttag tggaagattt tttcaaaaac
     6001 cctaaaagtt atttggtaat gatgaagtgt tttccatggt cgcataatga taaagttgca
     6061 ctgattggag attctgcgca cgccatcgtt cctttttatg gtcacggtat gaatgcgggt
     6121 tttgaagata ttactatttt aaatgaaatg attgcaaaat atggcgatga ttgggagaaa
     6181 atatttcaag aatatgaaca atctcgaaaa ccaaatgccg atgctattgc tgaattgtca
     6241 ttcagaaatt ttatggaaat gagttctaaa acggccgatg aaaacttttt attgcaaaag
     6301 aaaatagaaa aatggttttc tgataaacac ccagataaat ggatgccttt atacagtaga
     6361 gttactttta gtttacaacc ctattcagaa gcattagcta ttggcgaatt ccaaaataaa
     6421 attatggaac aagtaatgca atttccaaat atccaacaca attggaatag cgatgaagtt
     6481 gaaaaattaa ttttagagaa tttaaaatat aaaatttaat attttaatta ttctcggttt
     6541 actttttcaa aatcaaaagc aggattacaa atagcaatat attcacaagg ttcatcaaat
     6601 ggattggaat attgaactcg tgtatttttt tcaattttaa tggattgtcc tgcttctaaa
     6661 atcacagttt caccatcgat gataaattgt ttttttccag aaataataaa agtgtattca
     6721 tcaaattcgg gtgtttgaaa aggttcgctc cattttggag gagccatcat gtgagcaatt
     6781 gaaatctcag aattatttgt cgcaactttt ccgtgatgct ctttaattat ttttccatca
     6841 attgtaggaa caacaaaagg cgatttttga ataaaatatt ttttcataat tattttttaa
     6901 aaatgaaata cactgctaat atcaaaaagc aaaatccaac gaaatgattc catttaaaag
     6961 tttcattttt gaaaaaaaac agagaaaatc caacgaatac aaccaatgtt attacttctt
     7021 gaatgacttt taattgtaat aaagaaaatg gcccattatt tccttcataa ccaattttgt
     7081 tagcaggaac ttgaaaacaa tattcaaaaa aagccaagcc ccaactgatt aaaacgattg
     7141 taagtaatcc agcattttca aaccatttta attctttaaa ttttaaatga ccataccatg
     7201 ctaaagtcat gaaaatatta gaaagtataa gtaatccaat tgtaatataa cctttcataa
     7261 agcaaatgtt aaaaactaat cactattcac taattacttt tcactaaaca cctatttttt
     7321 aaacatttta gatagaattc caaaaacacc tctaataaaa gtagcacttg taacaacttt
     7381 tgtaacggac tttccaatta cttctgcagt actaggttca tttgcttttc tttttgttgt
     7441 agttttgatt tcttctgctt ttgctgcttc ttcagaagct ttttgattgg cttcgtcaat
     7501 ttttttagtc aaaatttcat aggcactttc acgatcaatt tcttcattat attttttaac
     7561 taattttgat ttttcattta ttgcatcaat ttccgattcg cttaaaatat ccattctact
     7621 cattggtgca cgaagcatgg tagcaactaa tggagttggg attccttttt cgtttaaggc
     7681 agttacaaat gcttctccaa ttccaagcga agtcaatact tcgtctgttt tatagtattt
     7741 agtgatagga taattttcgg cagtttgctt gatggcttgc ctatcttttg ccgtaaaagc
     7801 acgtaaagca tgttggattt ttaagcctaa ttgtgccaaa acactagcag gaacatccat
     7861 tggattttga gtgacaaaat aaacaccaat tcctttagaa cgaattaatt ttacaatagt
     7921 ttcaatttga tctaataaag ttttactagc ttcattaaaa atcaaatgtg cttcatcaat
     7981 aaaaatcacc aattcaggtc tttcggcgtc acctttttca ggcatttgtt ggtatatttc
     8041 agccaataaa ctcaacatga aagtagaaaa aagtttcggt ttatcttgaa tgtcagtcaa
     8101 ccgaataata tttacatatc cttttccgtt ttcatcaatg cgcattaaat catcaatttc
     8161 aaatgatttt tcaccaaaga aaatatcgcc accttgttgt tctaattcta taatctttct
     8221 taaaataatt ccagtagtcg aggtggaaat tttaccataa tgttcttcaa tttcagattt
     8281 tccttcttct gtgatgtaat tgattacttt ttttatgtct tttaagtcca aaagcggcat
     8341 ttgtttatca tcgcaaaatt tgaagattac cgaaacaaca ccagattggg tatcatttaa
     8401 atcgagtatt cttgaaaaaa ggactggacc aaattctgaa acggttgctc ttaatcggac
     8461 gccattttga gaagaaagcg acattaattc aactggaaat ttttgtatag tataaggtag
     8521 gtttatttta gcatgtcttt cggtaataaa agatttttct tcaccttcgg cagcaatacc
     8581 agagaaatca ccttttatgt ccatcattaa tacagggatg ccttgtgctg aaagttgttc
     8641 ggaaaaaacc tgaatggttt ttgtttttcc agttccagtc gctccagcta ttaaaccatg
     8701 tcgattcaat gtttttaatg ggattttaac tagtgtgtct gacaaagcat ttccatcaag
     8761 aattgcgcca ccaagaataa tattttcacc tttagtatta taattagtgt taatatattc
     8821 tgaaaattca gtctcttttg acatttgaaa gggtttttta attaaattta tggcaagtta
     8881 ttactatttg ttataaaata aaagttattt tttttcaatt tttatacttt tattgaaagt
     8941 taaaaaaatc attttataat atactttttt tgatattatt tcgttatact ataaactgtt
     9001 gaagtataat tgattctagt ttttattttt ttcaaacaaa aaaattaagg ttgttgcata
     9061 tcgactctat gttaatttaa agtaaattct aaattacaat tgttaaaaaa agttttttta
     9121 ttagaagtaa aaaaattaaa tttgctccta ttcaagtttc ttaaaaacta aaactaaaaa
     9181 aaataaatta aaactattaa aatttaaaaa aatggcaaac gtaaaaaaag agagcagttc
     9241 taatggagga aatattttct caggacttgt aattgtagga tgtgtacttg ttggaatcct
     9301 tgtgtggaaa ttcattatgg gtgatggttc taactttgaa ggaggagtta ataccggaca
     9361 tccacttcca ggaaattact tagcgatggt atataaaggt ggagctgttg tgccagtatt
     9421 gatgggatta ttcttaatgg taattgtgtt ttcttttgag cgttttttcg ttatcagtaa
     9481 agctggtgga aaaggaaatt tagatacttt catggcaaat gttcaagcaa gtattaaaca
     9541 aggaaacatt gatgacgcta ttgctgcttg tgataaacaa caaggttcgg tagccaatgc
     9601 tattaaagca gcattagtaa aatatcaaga tgttaagaaa gaagggttta atagtgaaga
     9661 tgctgctgaa acaattcaga aagaaattga agaagctact tctttagaaa tgccaatgtt
     9721 agagaaaaac atgacaattc tttctacatt agtgtcttta ggaacattag ctggtttatt
     9781 aggaacagta actggtatga ttaaagcatt tggtgcgtta gcatctgcag gaacacctga
     9841 ccaagctgcg ttagcaaatg gtatttctga agcacttata aatacagcta ctggaatttc
     9901 tacatctgct cttgcaattg ttacttacaa cttctttact tctaaaatag acacattgac
     9961 ctattcaatc gatgaagcag gtgcaacaat tgtaaatact tatagaaaat tcagaggaag
    10021 tttaaaatca taatcaataa ttagataact aactattgta taaaaaaaat aaaaaatggc
    10081 aaaagtaaaa atgtccaaaa agtcaacatc tatcgacatg acagcaatgt gtgatgtggc
    10141 tttcctttta cttacattct ttatcttaac ggcaaccgca aaaacgccag aaccacttcc
    10201 tgttgaaacg cctaattcta ctgtgcaaac aaaattgcct gaaacaggat tagtaacaat
    10261 aacaatagga aaaagtgatg gtaaaaatca ggttttcttc gggatgaaag atagagatgt
    10321 acgtgtaggt gctttggaat atatgtctga aaaatacgga gtaaccttta cagacaaaca
    10381 aaaagcgcaa tttgaattgg ttgaagaatt tggtgtacca atagagcaat taggcagtat
    10441 attagatatg aaagctgcag atagagcaaa agttggagtt caatcaggaa ttcctttaga
    10501 ttctatagga gaaaataaac aacaacttca agattggatt caatacgcca gaaaagcaaa
    10561 tatagatcga ggtgcaacca aagaacttga ttttgctata aaaggcgatg ctaaagaaga
    10621 atatccacaa attaaacaaa tcatggatat tttgcaaaac caaaaaatca ataacttcaa
    10681 tttagtgacg ggtttaagag gaaaagatta ttaattgtaa taatataaaa aaatggctga
    10741 attaaatacc ggcgacggcg gtggcaaaaa aggcggcgga aaagtaagaa gtaaaaaatc
    10801 caactcaaaa gttgatttaa ccgctatggt ggatttggca ttcttattga ttacgttctt
    10861 tatgttgacc acttccttat caaaacctca atctatggat ttgggtttac cagataaaga
    10921 tgaaaaagta gatactaata ttaaagtaga tcaagttcgt acgatgacaa ttattttagg
    10981 agaaaacaat aaaattaaat ggtaccacgg attattagaa tctcctgaac ctaatgggaa
    11041 acctacagat gctacttatg gaaaagacgg aattagaaaa gaaattttaa aaagagtaaa
    11101 atctattcct atcgctacag gtaatccaaa aaaaggaatg attgttatca ttaagcctac
    11161 taaaaaatca acatatagaa atttagttga tatattggat gaaatggcca tagcaaatgt
    11221 accaacgtat gctatagtaa atgatattac tccagaagag atgaaactgg ttgatgagat
    11281 gaataagtaa tgccttgtgc taatcttaaa aatataaaga aatgaaatta gacttattta
    11341 agaaaaaatg gattgacatc gtttttgaag gacgaaacaa agcctatggt gcttatgaac
    11401 ttcgtcaaga aaatccaaaa acgacaattc gttcatttat aataggagca tttatttttg
    11461 gattagctgt aggcgcaccg cttattgcta gtttaattcc tgacagtcag gaaaaagata
    11521 cttctttgga taaaaaaata gtaactatca agttgccacc aaagaaagaa gctccaaaaa
    11581 atcttccacc accaccaccg ccaccaccaa aagtggatca ggtaaagttt gtaaaaccag
    11641 ttgtggctaa aacggaagag attgttgaag aaccaccaaa gattgttgaa atcaaagaga
    11701 agaagcttgg tgccgaaaca attaagggtg acccagatgc accactaacc attgaaccag
    11761 taggaaatgg tccaagtaaa gtagtagaag aagataatag tatttataat actgcgggta
    11821 ttcaagtaca accagaattc ccaggaggaa tggataaatt ttacaagttt attggtaaaa
    11881 attatactcc accagatgaa gaaggtttga aagggaaagt ttttgtaact tttgttgttg
    11941 aaaaagatgg tactttaacc gatattaaag tgttaagaga tattggttat ggaactggta
    12001 aagaagcaat cagagtttta aaggcatgtc caagatggaa tccaggtgaa caaaatggta
    12061 aaaaagtaag ggtacaatat tcattaccaa ttacgattca atcggcttac taaatggttt
    12121 caaaaattat agataatttc aagaagaaat cgcttaatca gcggtttctt ctcgttataa
    12181 gaatgctgtt ttttttgatt tatttaacat ttggtctggc agttatcttt tggaaacaat
    12241 taccgcttaa tttaacacaa ccttataaaa ttgcattagg cttacttatg attgtgtatg
    12301 cattttttag atttttaaga tatttatata tagatgaaaa taatgaataa aaaacatatt
    12361 ttctttattg ttttatcatt tgtctttgtc attttttttg cttgtaaaaa aaataaccaa
    12421 gatgaagaaa ctataataaa aggaaaagcg acacttttgg ttgatgaatc catctttcca
    12481 atcgttgaag accagcaagc agtttttgaa acacaatatg atgcgaagtt aagtttagtt
    12541 agaaaatcgg aagctgagat tattaattcc ttattgaatg acacactcaa aattgcggtc
    12601 ataccacgaa aacttaccga tgcagaagac aaagcttttt caaacaagaa gatttttcca
    12661 aaacaaacaa tttttgcaaa agacgctgta gtttttataa ccaataaaat gtcaaaggat
    12721 agcttgattg ctttagaaga tattgtgaaa ttcatgaaag gtgaagatgt taaaggaatt
    12781 aaaggattag tttttgataa tccaaattca agtactgttc gtttgctttc agaaaaagca
    12841 ggaataaaag tgacgcccca aaaaaatatt ttttcttttg ctaccaataa tgaagtgatt
    12901 aaatatgttt cccaaaacga aggaatgatt ggtgttgtag gtgttaattg gttgtttcaa
    12961 ccaccattag atatgcaacc aacagtagat aaagttagca ttatgagcgt taaatcagac
    13021 gaaaaatttg gttttgttta tccaagtcaa gagaatattg gtaacggaaa atatcctttg
    13081 gcacgccatt tgtatattat aaactgtcaa ggatattcag gattaggaat gggatttgca
    13141 tcctttcttg gtggcgaacg cgg
//
LOCUS       DAOOBH010000063        12907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_6235_length_12907_cov_5.864535, whole genome shotgun sequence.
ACCESSION   DAOOBH010000063 DAOOBH010000000
VERSION     DAOOBH010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6235_length_12907_cov_5.864535"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1995)
                     /gene="porU"
                     /locus_tag="PK218_07480"
     CDS             complement(<1..1995)
                     /gene="porU"
                     /locus_tag="PK218_07480"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPW98384.1"
                     /translation="MKKNMKYLLLVLCGFLGFAQEKGSVVLNWKNKEIYSNERSNYVI
                     PQFNQEFFRFSFYDKTMTCVVTITNGINQRSDVEIINPVFETIPESYLGDLNKEKFPN
                     SLKSDFNFITARDKQFGQLNVSPIIKENNSYKKLLSFEYRISQSTARTYVSNNVSAIE
                     SSVLATGDWYRFYVEKSGVYKISKGFLNSLGMNTNADPRKIKIFGNGGRMVPLLNSEE
                     YPAGLEENAIKFVGESDGIFDDQDYILFYAEGVDNWSQENQTNLNLYANKSYYYVTVS
                     GSEGKRIVDLTQPDDNPTVTINTFDNYKFHEVDKENIAKLGRVWFGEQFSIDNSQDFD
                     FDFPNLVTSSPVNFRIHLAAASAVITSFKAEVNSQIIGTSNFSAISANSSTEAIDNYI
                     SNNIPITNNTFSVKITYDNGGAPNSKGYLDYINVKAKCELKGTSKQFRFQFDDAASLI
                     GVGKFDISNASSINEVWDITDIFNVTKTENVGASNTFSFKTNLGEIRKYIAVVSSDYY
                     FPLKESKARVSNLDLKGTVFKNNQGQFQDVDYLIFTPRNLNSQAEKLANFHRNNSQLN
                     VKVINLDDVYTEFSSGKQDIGAIRNFIKYVYFNASSSDKRIKYVNLFGDASYDFKDRI
                     SGNTNIVPIYQSLNSYTEGESSFASDDFYGLMDANEGRIDF"
     gene            2203..3945
                     /gene="gldJ"
                     /locus_tag="PK218_07485"
     CDS             2203..3945
                     /gene="gldJ"
                     /locus_tag="PK218_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HPW98385.1"
                     /translation="MKYFKTMNIRLILLAMLSLSVVSCSKKSSSKGGSQATGWKINDK
                     KGGFQYASKFKKQATGPGLVLVEGGTFTMGKVQDDVMHDWNNTPNQQHVQSFYMDETE
                     VTNMMYMEYLDWLKRVFPPEQENYKNIYEGASPDTLVWRNRLGYNETMTNNYLRHPAY
                     AEYPVVGVNWIQATEFAKWRTDRVNEKTLEEQKYLKKDAKITDVSSDKVFSTEAYLAS
                     PSTAMGGDSELVLQKGKGRGTSKKSSDGGADAAAGSTNNQGNSPKNVYAQRSSGLILP
                     DYRLPTEAEWEYAAAADVGQREYNLYKGQKKYPWSGSYTRSGKRQVKGDQLANFKQGK
                     GDYGGIAGWSDDGADITNKVKSYPPNDFGLYDMAGNVAEWVADVYRPIIDDEANDFNY
                     YRGNVYMKNKIGEDGKVELVTAENQKFDTLSNGKIIARNYPGEIAQVPVDENETYLRQ
                     NFDKSDNRNYRDGDKQSSRYFKFGASEEGDEKGQLKDNQRMYDSPKHNVSVDSLGSMV
                     RKYDKSNKRTTLINDDVRVYKGGSWRDRAYWLDPAQRRYYPQDIATDYIGFRCAMSRV
                     GPKSDKRKSARNKR"
     gene            4012..5295
                     /gene="murF"
                     /locus_tag="PK218_07490"
     CDS             4012..5295
                     /gene="murF"
                     /locus_tag="PK218_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPW98386.1"
                     /translation="MEIKQLHDNFLECQSFSTDTRKIEKNSMFFALKGDSFDANTFTI
                     EAFEKGAKFVVIDNPAYYIDSKTILVKDVLKTLQELATYHRNHLGLPIIALTGSNGKT
                     TTKELINVVLSKKFKAKATVGNLNNHIGVPLTLLSFNKETEIGIVEMGANHQKEIEFL
                     CAIAQPDFGYITNFGKAHLEGFGGVEGVIKGKSEMYTYLFNNKKVVFINEDDTIQKNK
                     TLNYRLKYSFSKIDKKANVFIDSIDANPFVKIETTGVCIQSNLIGLYNANNITAAITI
                     GSYFKVPLLDCKDAIESYVPKNNRSQLISKGTNEIILDAYNANPSSMIVALENFLQLE
                     KVNKLIIIGDMYELGEESLMEHNIIVSYLKDKNSLTYFIGKNFYSNKVTSPFFQFFAT
                     FEELSNHLEKSKPENKFILIKGSRGMALERTLEFI"
     gene            complement(5324..6358)
                     /gene="obgE"
                     /locus_tag="PK218_07495"
     CDS             complement(5324..6358)
                     /gene="obgE"
                     /locus_tag="PK218_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPW98387.1"
                     /translation="MTEGNFVDYVKIYVSSGKGGKGSSHLHREKFIEKGGPDGGDGGR
                     GGHVIVRGNKGLWTLFHLKFARHIKAGHGGDGGSSRSTGADGEDKYIEVPLGTVIRDK
                     ESNEILFEITENGEEYILAKGGKGGLGNWNFRSSTNQTPRYAQPGMPTEELDVILELK
                     VLADVGLVGFPNAGKSTLLSVLTSAKPKIADYPFTTLKPNLGIVAYRDFQSFVIADIP
                     GIIEGAAEGKGLGHYFLRHIERNSTLLFLVPADAEDIKKEYDILLDELRRYNPEMLDK
                     DRLIVVSKSDMLDEELKSEMKEELDQAFKEIPYMFISSLANQGLTQLKDKLWQMLNID
                     TELPENSHGI"
     gene            complement(6429..7703)
                     /locus_tag="PK218_07500"
     CDS             complement(6429..7703)
                     /locus_tag="PK218_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPW98388.1"
                     /translation="MEILVIFFLILLNGVFSMSEIALISARKNRLENAAKKGNKNAKV
                     ALELANSPNTFLSTVQIGITLIGILTGIFSGDKITTDVRTFVEGFAFLKPYADSIAVG
                     IVVVILTFFSLVLGELLPKRIGLNYPEKIAKGVAVPMKVISLVTKPFIWMLTKSTEFI
                     LDVFKIKPSADGKVTEEEIKAIIKEGTEGGEVQEIEQDIVERVFHIGDRKVNSLMTHR
                     KAIEYLTIEDSLVEIKEKVFAELHSVYPVCKDNLDEIIGVVYLKEMILNFEKGNFDLN
                     KIIKEPVYMIEHTSAYKALENFKLSKIHYALVTDEYGIFQGIITLNDILEALVGDASE
                     FYGDEFKLLAREDGTWFVDGHYSLHDFMTYFEMDDMISDYDVTTVSGLIMTEMGKIPK
                     QGEKIIWNKYELEVIDMDGIKIDKVLVKALKE"
     gene            complement(7797..8369)
                     /locus_tag="PK218_07505"
     CDS             complement(7797..8369)
                     /locus_tag="PK218_07505"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPW98389.1"
                     /translation="MINIVLFGKPGAGKGTQAEFLKEKYNLTHLSTGDIFRYNIKNET
                     ELGKLAKTYMDKGDLVPDEVTIKMLQSEVDNNPKSAGFLFDGFPRTIAQAKALDAFLK
                     TKKQSITATVALDADDEVLVKRLLERGKTSGRPDDQDEEKIRNRYEEYNQKTAPLTDY
                     YKAQDKFYAVDGIGTIEEVTARLSKVINNL"
     gene            complement(8416..8946)
                     /gene="hpt"
                     /locus_tag="PK218_07510"
     CDS             complement(8416..8946)
                     /gene="hpt"
                     /locus_tag="PK218_07510"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPW98390.1"
                     /translation="MIHLHDKTFEPFISSEEISFAIKNMAKKMDDDFFDDVPVFIGVL
                     NGSFMVLSDLMKEYRGMCEVSFLKLASYEGTQTTHKVKQLIGLNQDLTGRTVVVVEDI
                     VDTGNTIEELKSIFKEKNVKHLKFATLFFKPDAYTKDIKIDYIGMRIPNKFIVGYGLD
                     YDGLGRNLPDVYQLAQ"
     gene            9042..9575
                     /locus_tag="PK218_07515"
     CDS             9042..9575
                     /locus_tag="PK218_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98391.1"
                     /translation="MNSDYYHQIAKSTAHRKSRDENKNFIFEHPEFLKGLVEIAFQTK
                     DKNHHKACWILELVCEERMDLFYPYIDEYCNVLKDYKSDSAIRSVSKIGLFLVKNKKI
                     QLTEIQEGKIIETFLDWLILSNKAANAAYSMRTLFILGKKHPWVNDELKLLLSRDCSH
                     QTPGYKFAVKDILKRLK"
     gene            9644..>10641
                     /locus_tag="PK218_07520"
     CDS             9644..>10641
                     /locus_tag="PK218_07520"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HPW98392.1"
                     /translation="MNYFSSDFKLGILGGGQLGKMLLSDTRKFDIQTFVLDPSDEAPA
                     RLACNNFFQGDLLDFDTVYRFGKLVDVLTIEIENVNLDALEKLEKEGLKIYPSPKTLR
                     LIQSKAVQKEFYIKNKIPTAKGSAFKNLKELLVEFMDGKLKMPFVWKSARFGYDGNGV
                     KIIRTPKDLETLPDVECIMEELVPFKNELAVIVVRNVKGEVKTYPVVEMEFHPEANQV
                     EYVICPARIDLPISLKAQEIALKVSKAYKHVGILAVEMFQTKDDKILVNEVAPRPHNS
                     GHYSIEASYTSQFENHLRAILNLPLGKTDSKVAGIMVNLVGEDGHTGNVKYKNIEK"
     assembly_gap    10642..10741
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10742..11221
                     /gene="purE"
                     /locus_tag="PK218_07525"
     CDS             <10742..11221
                     /gene="purE"
                     /locus_tag="PK218_07525"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPW98393.1"
                     /translation="KVAIIMGSKSDLPVMQEAIDILNSFDIQTEVDIVSAHRTPEKLY
                     DFSKNAHHRGISVIIAGAGGAAHLPGMVAAMSPLPVIGVPIKSSNSIDGWDSVLSILQ
                     MPSGVPVATVALNGAKNAGILAAQIIGSHDKCVLDKIIFYKESLKEAVNKSSEEMKK"
     gene            11245..11472
                     /locus_tag="PK218_07530"
     CDS             11245..11472
                     /locus_tag="PK218_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98394.1"
                     /translation="MDIQTSKIELAKVILDIENPKLIEEIILLIQSKSSLTDSQKKAI
                     DDAIYSLENNEGIRHEMVMEETKNRYSKYFK"
     gene            11473..11760
                     /locus_tag="PK218_07535"
     CDS             11473..11760
                     /locus_tag="PK218_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPW98395.1"
                     /translation="MEVIWSPQAKKDFWNNIDYLEAEWTFKDVVNFIEKVEKTINLLK
                     KSNINFVSANYKEVNKVVVIKQITLYYRLNSKTLELLRFWNTYQDLENFKL"
     gene            11801..>12907
                     /locus_tag="PK218_07540"
     CDS             11801..>12907
                     /locus_tag="PK218_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPW98396.1"
                     /translation="MKVLTQKFTTKYETAPFSQIKMEDYKPAFEENIKNAKAEIDTII
                     NNPDSPTFENTLEALDFAGYPLDRLSSIFFNLNSAETTDEMQKIAQEVSPLLTEFGND
                     ITLNEDLFKRIKAVYDAKDRLNLTTEQHTLLDKKYKGFVRNGALLNEEQKKKLREIDT
                     QLAKLKLTFGENVLAETNNYHLQITNEADLKGLPEGVVEAAKAEAENRQLDGWVFTLD
                     MPSYLPFMTYADNRELRKELAIAAGKKGFQNNEYDNQNNVKDIVRLRHERANLLGYAS
                     HAHFVLEERMAQNPEKVISFLNDMLEKAKPAAQKQFEELTAFAKELDGIDHLEKWDSA
                     YYSEKLKQKLFNLDDEKLKPYFKLENVLNGAFTIA"
BASE COUNT         4489 a         2081 c         2073 g         4164 t
ORIGIN      
        1 aaagtctatt ctaccttcat ttgcatccat caaaccataa aaatcatcgg aagcaaatga
       61 actttcgcct tctgtataac tgtttaacga ttgatatata ggcacaatat tggtattccc
      121 gctaattctg tctttaaaat cataagaggc atcaccaaat agatttacat atttaattct
      181 cttatctgaa gaagaagcat taaagtaaac gtattttata aaatttctaa tagctccaat
      241 atcttgtttg ccagatgaaa actctgtgta aacatcatca agattgataa cttttacatt
      301 taactgcgaa ttgtttctgt gaaaattggc taatttctca gcttgagaat ttagatttct
      361 tggtgtaaaa attaaataat ctacatcttg aaattgacct tgattatttt taaaaaccgt
      421 tccttttaaa tctaaatttg aaacccttgc ttttgattct ttcagtggga aataataatc
      481 cgaacttact acagctatat attttcttat ttcgccaaga tttgttttaa acgaaaaagt
      541 atttgaagca cctacatttt ctgttttagt tacattaaat atatcggtta tgtcccaaac
      601 ttcattaata cttgatgcat ttgaaatatc gaattttcca acacctatta aagaagcagc
      661 atcgtcaaat tgaaaacgaa attgtttaga cgtacctttt aattcgcatt ttgcttttac
      721 atttatataa tctaaatatc cttttgaatt tggtgcgcca ccattatcat aggtaatttt
      781 tacgctaaac gtattgttgg taataggaat attatttgaa atgtaattat caatagcttc
      841 tgtacttgaa tttgcagaaa tagcactaaa atttgatgtt ccaataattt gactgtttac
      901 ttcggcttta aaactagtaa taactgctga ggcagcggct aaatgaattc taaaattaac
      961 tggggaagaa gttactaaat ttggaaaatc aaaatcaaaa tcttgcgaat tatcaatcga
     1021 aaattgttcg ccaaaccata ctcttcctag ctttgcaatg ttttctttat caacttcatg
     1081 gaatttataa ttgtcaaacg tatttattgt aaccgttgga ttatcatctg gttgcgttaa
     1141 atcgacaatt cttttacctt ctgagccact aactgtcaca taataatagg acttgttggc
     1201 atataagttc aaattagttt ggttttcttg actccaatta tcaacgcctt ctgcatagaa
     1261 aagaatataa tcttgatcat caaaaatccc gtcggattca ccaacaaatt ttattgcatt
     1321 ttcttctaaa ccagcagggt attcttcaga atttaacaac ggtaccatcc gaccaccgtt
     1381 cccaaagatt ttaatttttc ttgggtcggc attggtattc atccctaaac tattaagaaa
     1441 acctttagaa attttataga cacctgattt ctcaacataa aaccgatacc aatcaccagt
     1501 tgctaagaca gaactttcga ttgcagaaac attattacta acataagttc tggcagtact
     1561 ttgagaaatc ctgtattcaa atgaaagtag ttttttataa ctattgttct ccttaataat
     1621 aggtgacaca ttaagttgac caaattgttt gtcacgtgca gtaataaaat taaaatctga
     1681 ctttaaacta ttaggaaatt tttctttatt taagtctcct aaataacttt ctggaatggt
     1741 ttcaaaaacg gggttgatta tttcgacatc acttctttga ttgataccgt ttgtaatagt
     1801 aactacacaa gtcattgttt tgtcataaaa actaaaacga aagaactctt gattgaattg
     1861 aggaataaca tagttacttc tttcattaga ataaatctct ttatttttcc aatttaacac
     1921 aacacttcct ttctcttggg caaaccccaa aaaaccacac aataccagta gcaagtattt
     1981 catattcttt ttcatattta taaagtaaac gaaaataatt aatgtttatt gtattttttg
     2041 aaatattttt taaaatcgta caataaaaaa actacttttg cgttattaat tacgtaaaca
     2101 gtatataaaa cgtttagcga aattagtatt aattaaaaaa atttgttgct aattaaaggt
     2161 taattcttaa attgcgccac aaatctgtaa cctacaatgg atatgaaata ttttaaaact
     2221 atgaatatta gactaatact tttagcaatg ttgtctttaa gcgttgttag ttgtagcaag
     2281 aaatccagct caaaaggagg atctcaagca acaggatgga aaatcaacga caaaaaaggt
     2341 ggtttccagt atgcttcaaa atttaagaaa caagccacag gaccagggct tgtattagta
     2401 gaaggtggaa cgtttaccat gggtaaagtt caggatgatg ttatgcacga ttggaacaac
     2461 actccaaacc aacaacacgt tcagtctttt tacatggacg aaacggaagt taccaatatg
     2521 atgtatatgg aatatcttga ttggttaaaa agagttttcc ctcctgaaca agaaaattac
     2581 aaaaacattt atgaaggtgc ttcacctgat acacttgttt ggagaaatcg tttaggatat
     2641 aacgagacga tgaccaataa ctatttaaga catcctgcct atgcagaata tccagtagtt
     2701 ggagtaaatt ggattcaagc aacagaattt gcaaaatgga gaaccgaccg tgtaaacgaa
     2761 aaaactttag aagagcaaaa atacttaaaa aaagatgcta aaattactga tgtaagttca
     2821 gacaaagtat ttagcactga agcttatttg gcatctcctt caacagctat gggtggcgat
     2881 tcagaattgg tgcttcaaaa aggaaaagga agaggaactt caaagaaatc aagtgatggc
     2941 ggtgctgatg ctgcggctgg aagcacaaac aatcaaggaa atagtcctaa aaatgtgtat
     3001 gcacaaagat catctggttt gattttacca gactatagac taccaactga agcagaatgg
     3061 gaatatgctg ctgctgcaga tgtaggtcaa agagagtaca atttatacaa aggacaaaag
     3121 aaatatcctt ggtctggaag ctacactcgt tctggaaaaa gacaagttaa aggtgatcaa
     3181 ttggcgaact ttaaacaagg taaaggtgat tatggtggta tagcaggttg gtctgatgac
     3241 ggtgctgata ttacaaacaa agttaaatct tatcctccaa atgattttgg attatacgat
     3301 atggcaggaa acgtagctga atgggttgca gatgtttaca gacctattat agatgacgaa
     3361 gcaaacgatt tcaactatta tagaggaaac gtttatatga aaaataaaat tggtgaagat
     3421 ggaaaggttg aacttgtaac tgccgaaaat caaaagtttg acacattatc taacggaaaa
     3481 attattgcac gtaactatcc tggtgaaatt gctcaagttc cagtagatga aaacgaaacc
     3541 tacttaagac aaaactttga caaatctgac aatagaaatt atagagatgg tgacaaacaa
     3601 tcctctagat attttaaatt tggagcttcg gaagaaggtg atgaaaaagg tcaattaaaa
     3661 gacaaccaaa gaatgtatga ctctccaaaa cacaatgtat cggttgacag tcttggaagc
     3721 atggtaagaa aatacgacaa gtctaacaaa agaacaactt taatcaatga tgatgtaaga
     3781 gtttacaaag gtggatcatg gagagacaga gcgtactggt tagatcctgc acaacgcaga
     3841 tattatccac aagacatcgc tactgactat atcggattta gatgtgccat gtctagagtt
     3901 ggtccaaaat cagataaaag aaaatctgca agaaataaaa gataatattt tttttgataa
     3961 attaataaaa gccctatttt tagggctttt attgtatatt atacattagt tatggaaata
     4021 aaacaattac acgacaactt tttagaatgc caatcttttt caaccgatac tcgaaaaata
     4081 gaaaaaaatt ccatgttttt tgctttaaaa ggggatagtt ttgacgctaa cacttttact
     4141 atagaagcat tcgagaaagg tgcaaaattt gttgtaatag acaatcctgc atattatata
     4201 gattctaaga caatacttgt caaagatgtt ttaaaaacat tgcaagagtt ggcaacttat
     4261 catagaaacc atttaggatt gccaattatt gctttaacag gtagcaatgg taaaacaacc
     4321 accaaagaac tcattaatgt tgttttatca aaaaaattca aggccaaagc aacggttggc
     4381 aatcttaaca atcacattgg agttccatta acactacttt catttaacaa agaaactgaa
     4441 atcggcattg ttgaaatggg cgcaaatcat caaaaagaaa ttgaattttt atgcgccata
     4501 gcacaacctg attttggata cattacaaat tttggaaaag cacatttaga aggttttggc
     4561 ggagttgaag gtgtaattaa agggaaaagc gaaatgtaca cttatctttt taacaataag
     4621 aaagtggtct tcattaatga agatgacacc attcaaaaaa acaaaacatt aaattatcgt
     4681 ttaaaatatt cctttagtaa aattgacaaa aaagcaaatg tatttataga ttcaattgac
     4741 gcaaatccat ttgttaaaat agaaacaacc ggagtttgca ttcaatcgaa tttaatcggt
     4801 ttgtataatg ccaacaatat tacagctgca attactattg gaagttattt taaagtgccg
     4861 ttactagact gcaaagatgc gatagaaagc tatgttccaa aaaacaaccg atcgcaacta
     4921 atcagcaaag gaactaatga aattatctta gatgcttata atgctaatcc aagtagtatg
     4981 attgttgctt tagaaaattt tcttcaacta gaaaaagtca ataaattaat tattattggc
     5041 gatatgtatg aacttggcga agaaagtcta atggaacata atataatcgt ctcttattta
     5101 aaagataaaa atagtctaac ttattttatt ggaaagaatt tctactcaaa taaagttacc
     5161 agtccttttt ttcaattttt tgctactttt gaagaactta gcaatcatct agaaaagtca
     5221 aaaccagaaa acaaatttat tttaataaaa ggttcaagag gaatggcatt agaacgcact
     5281 ttagagttta tataaatcaa aaaatcattt cgttgggttt tgattatata ccatgactgt
     5341 tttctggaag ttcagtatcg atattaagca tctgccacaa cttatcttta agctgcgtta
     5401 atccttgatt ggctaatgaa gaaataaaca tgtaaggaat ttctttaaac gcttgatcga
     5461 gttcttcttt catttcggat ttcaattcct catcgagcat atcagatttt gaaacgacta
     5521 tcaatctatc tttgtctaac atttcaggat tgtaacgacg aagttcatct aacaaaatat
     5581 catattcttt cttaatgtct tctgcatccg caggaacaag aaaaagcaac gttgagtttc
     5641 gttcaatatg acgtaaaaaa taatgtccta atccttttcc ttcggcagca ccttcgatga
     5701 ttccaggaat atcagcaatc acaaacgatt gaaaatcgcg ataagcaact attcctaaat
     5761 taggttttaa agtagtaaat ggataatcgg caatctttgg tttggcagaa gttaacacag
     5821 ataataaagt tgattttcct gcgttgggga aacctactaa accaacatcg gccaaaactt
     5881 taagttctaa aattacatcc agttcttcgg ttggcattcc tggttgtgca taacgaggcg
     5941 tttggttagt cgaacttcta aaattccagt ttcccaatcc tccttttcct cctttggcca
     6001 atatatattc ttcgccattt tcggtaattt caaatagaat ttcgttgctt tctttatcac
     6061 ggataacagt tcctaatggc acttcgatat atttgtcttc gccatcagca ccagtactcc
     6121 tactgcttcc gccatcgcct ccatgtcctg ctttaatgtg tcttgcaaat tttagatgaa
     6181 acaatgtcca aagtcctttg ttacctctta caattacgtg tccgcctcta ccaccatctc
     6241 caccatctgg accacctttt tcgatgaatt tttcacgatg caaatgagac gaaccttttc
     6301 cgccttttcc agatgaaaca tatattttta cgtaatctac aaaatttcct tctgtcataa
     6361 tttaagtttt tagtgattaa cattcaaaat ttagtggtta ttaccaacta agaactgaat
     6421 attatcagct attctttaag tgcttttact agcactttat caatttttat accatccata
     6481 tcaatgacct ctagctcata cttgttccaa atgatttttt caccttgctt gggaattttt
     6541 cccatttcag tcataatcaa tccacttacg gttgtaacat catagtcgga aatcatatca
     6601 tccatttcaa aataagtcat gaaatcgtgt agtgaataat gcccatctac aaaccatgtt
     6661 ccatcttctc gcgctaatag tttaaattcg tcaccataaa attctgaggc gtcacctaca
     6721 agtgcttcta aaatatcatt taatgtaatt attccttgaa aaatgccgta ttcatccgtt
     6781 accagcgcat aatgaatttt ggataattta aagttttcca aggctttata ggcagaagta
     6841 tgttcaatca tgtaaacggg ttctttgatg attttattta aatcaaagtt gcctttttca
     6901 aaattaagaa tcatttcttt tagataaacc actccaataa tttcatccaa attatcttta
     6961 caaacaggat atacagaatg tagttcggca aaaacttttt cttttatttc cacaagggaa
     7021 tcttcaattg tcaggtattc aattgctttt cgatgtgtca ttaaagagtt aacctttctg
     7081 tcgcctatgt gaaaaactcg ttcaacaata tcttgttcaa tttcttgtac ttcgcctcct
     7141 tcagttcctt ctttaatgat ggcttttatt tcttcttctg ttacttttcc atcggcagat
     7201 ggtttgattt taaaaacatc caaaataaat tctgtagatt ttgtcaacat ccaaatgaaa
     7261 ggcttggtga ctagcgaaat cactttcatc ggaacagcaa cgccttttgc tattttttca
     7321 ggataattta atccgattcg ttttggcaat aattcaccta aaactaaaga gaaaaaagtc
     7381 aaaatcacca ctacaattcc aactgcaatg ctatctgcat atggctttaa aaacgcaaaa
     7441 ccttctacaa aagtacgaac atcggttgta attttatctc cagaaaaaat acctgttaat
     7501 attccaatta acgtaatccc gatttgaaca gtagaaagaa acgtatttgg tgaattggct
     7561 aattcaagtg ctacttttgc gtttttattt ccctttttag cggcattttc caatcggttt
     7621 tttcgtgccg aaatcagtgc aatttccgac atggaaaaca caccgtttag gagaatcaga
     7681 aaaaatatga ctagaatttc cattaattat gtttatgcgt ttagggagtt aaaaggatag
     7741 aaggttgata agatattatc gtgaatactt ttaaactcct aacctactaa ctttttttac
     7801 aaattattga tgactttact taaccgagcc gttacttctt ctatggttcc aattccatct
     7861 acagcataaa atttatcttg tgccttataa taatcggtta atggagctgt tttttgattg
     7921 tattcttcgt aacgatttct gattttttct tcatcttgat cgtcaggacg tccagaagtt
     7981 ttgcctcttt ctaacaaacg tttcactaaa acttcatcat cggcatctaa agcgactgta
     8041 gcggtaattg attgtttttt agttttaaga aatgcatcca aagccttggc ctgtgcaatt
     8101 gttcttggga aaccatcaaa taaaaatcca gctgattttg gattattatc cacctcgctt
     8161 tgcagcattt taattgttac ttcatcaggc actaaatcgc ctttgtccat ataagttttg
     8221 gcaagtttac ctaattcagt ttcattttta atattgtatc taaaaatatc tcctgttgaa
     8281 agatgggtaa gattgtattt ttcttttaaa aattcagctt gtgtgccttt tcctgcacct
     8341 ggttttccga aaagaacaat gttgatcatt ttaatcttag tttaatattg tttaaaagtt
     8401 taaatagttt aatttttatt gcgctaattg gtaaacatct ggaaggtttc tgcctaaacc
     8461 atcatagtct aaaccataac ccacgataaa tttattagga atacgcattc cgatgtaatc
     8521 tattttaatg tctttcgtat atgcatcagg tttgaagaat agcgttgcga attttaaatg
     8581 ctttacattc ttttctttga atattgattt taactcttca atagtgtttc cagtatcaac
     8641 aatgtcttcc accacaacga cagttcttcc cgttaaatcc tgatttaatc ctataagttg
     8701 ttttacttta tgtgttgttt gtgtaccttc gtatgatgct aactttaaaa aagacacttc
     8761 acacatgcct cggtattctt tcatcaaatc agacaatacc atgaacgaac cgtttaaaac
     8821 accaataaaa actggcacgt cgtcaaaaaa atcatcatcc attttttttg ccatattttt
     8881 tattgcaaaa gaaatttcct cagatgatat aaagggttca aaggttttgt cgtgaagatg
     8941 tatcatggct tagaatttta aataagttgc aaatttaaag aagtattaag tattaagaac
     9001 taagtattaa gacttttttg caaaattgtt atatttacat catgaatagc gattattatc
     9061 atcaaattgc caaaagtaca gctcatcgga aaagcagaga cgaaaacaaa aattttatat
     9121 ttgagcatcc agagttcctt aaaggcttag tagaaattgc atttcaaaca aaagacaaaa
     9181 atcatcataa agcttgctgg atattagagt tggtttgtga agaaagaatg gatttatttt
     9241 acccttatat tgatgaatat tgcaatgttt taaaagatta taaatccgat tctgctattc
     9301 gttctgtttc taaaatagga ctatttctag ttaaaaacaa aaaaattcaa ctcaccgaaa
     9361 tccaagaagg aaaaataata gaaacgtttt tagattggtt gattctatcc aataaagctg
     9421 ctaatgcagc ctattcgatg cgaactttat ttattttagg aaaaaaacat ccctgggtaa
     9481 atgatgaatt gaagttattg ctttcaagag attgtagtca tcaaactcct ggatataaat
     9541 ttgctgtgaa agacattttg aaacgattaa aataattctc aaatgtttat ctttgcaact
     9601 acactaacta aaacaaatct gattcacatt cagattatat acaatgaatt atttctcatc
     9661 cgattttaaa cttggaatct taggtggcgg acaattgggc aaaatgctat tgagtgatac
     9721 tcgaaaattt gacatacaaa catttgtact cgacccaagt gatgaagcac cagcacgact
     9781 agcgtgtaat aatttttttc aaggcgattt attagatttt gataccgttt accgatttgg
     9841 aaaattggtt gatgtattga ctattgaaat tgaaaatgta aatcttgatg ctttagaaaa
     9901 actagaaaaa gaaggtttaa aaatataccc atcgccaaaa acattacgtt taattcaaag
     9961 caaagcggtt caaaaagaat tttacataaa aaacaaaatt ccaacggcta aaggaagcgc
    10021 ttttaagaat ttaaaagaac tgttggttga gtttatggat gggaaattaa aaatgccatt
    10081 cgtatggaaa agtgctcgtt ttgggtatga cggaaatggc gtaaaaatta ttcgtacacc
    10141 aaaagacctt gaaaccttgc ctgatgtaga atgcatcatg gaagaattgg ttccttttaa
    10201 aaatgaactg gcagtaatag tagttagaaa cgtaaaaggc gaggtaaaaa cctatcctgt
    10261 tgtagagatg gagtttcatc ctgaagccaa tcaggtggaa tatgttattt gcccagcaag
    10321 aatagattta ccgattagtt tgaaagctca agaaattgcg ttgaaagttt ctaaagctta
    10381 caagcatgtt ggaattttgg ctgtagaaat gtttcaaact aaagatgata aaatattagt
    10441 aaacgaagtg gcaccacgac cacacaattc gggacattac tcaatagaag cgagttatac
    10501 ttcacagttt gaaaatcatt tgcgagctat tttaaattta cctttaggaa aaaccgatag
    10561 taaagttgcc ggaattatgg taaatttggt tggcgaagat ggacatactg gaaatgtgaa
    10621 gtataaaaac atcgaaaaaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 naaagtagcc ataatcatgg gttcaaaatc agatttaccc gtaatgcaag aagccatcga
    10801 tatattaaac agttttgaca tccaaactga agttgacatc gtttctgcac atcgaacacc
    10861 tgagaaatta tatgatttta gtaaaaatgc acatcataga ggaatttcag taattattgc
    10921 tggtgctggt ggtgcggcac atttgcctgg aatggttgcc gcaatgtcac cgcttccagt
    10981 aattggtgtt cctataaaat caagtaattc tattgatggt tgggattcag ttttgtctat
    11041 tctacaaatg cctagtggcg ttcctgtagc aactgtagcc ttaaatggcg cgaaaaatgc
    11101 tggaattttg gcggctcaaa tcatcggaag tcatgataaa tgtgtgctgg ataaaattat
    11161 tttttacaaa gaaagtttga aagaagccgt gaataagtcg tcggaagaaa tgaaaaaata
    11221 aatacctttg ataaaaagaa aattatggat attcaaactt caaaaataga attggctaaa
    11281 gttattttgg atatcgaaaa cccaaaatta attgaagaaa ttattctttt aattcaatct
    11341 aaaagttctt taactgatag tcaaaaaaaa gccattgatg atgctattta ttccttagaa
    11401 aacaatgaag gaattcgcca tgaaatggtt atggaagaaa ctaaaaatcg atattccaaa
    11461 tattttaaat aaatggaagt catttggtcg ccacaagcca aaaaagattt ttggaataat
    11521 attgattacc tcgaagcaga atggactttt aaagatgtag tcaattttat tgaaaaagtt
    11581 gaaaaaacaa ttaatcttct gaagaaaagt aacattaatt ttgtttcagc aaattataaa
    11641 gaggttaata aagtagttgt aataaaacag attacacttt attatagatt aaattccaaa
    11701 acacttgaat tacttcgctt ttggaacact tatcaagatt tagaaaattt taaattataa
    11761 atagaaataa aaataacccg aaattttggg aatataaaac atgaaagtac ttactcaaaa
    11821 atttactacc aaatacgaaa cagctccatt ttcacaaatc aaaatggaag attacaagcc
    11881 tgcttttgaa gaaaacatca aaaacgcaaa ggctgaaatt gatactatta tcaacaatcc
    11941 tgattcgcca acttttgaaa acactcttga agctctagat tttgcaggtt atcctttaga
    12001 tagattatcg tctatttttt ttaatctgaa ttcggctgaa actactgatg aaatgcaaaa
    12061 aattgctcag gaagtttcgc cattattaac cgaatttgga aacgatatta ccttaaatga
    12121 agatttattc aaacgtataa aagcagttta tgatgctaaa gaccgtttga atttaactac
    12181 cgaacaacac actttattgg ataaaaaata taaaggattt gttagaaatg gagcattgct
    12241 aaacgaagaa caaaagaaaa aacttcgtga aattgacacg cagttagcca aattaaaact
    12301 gacttttggt gaaaatgtat tggcagaaac caacaattac catttgcaaa ttactaatga
    12361 agcagattta aaaggtttac ccgaaggtgt tgtagaagct gcaaaagcag aagccgaaaa
    12421 ccgacaattg gatggttggg tttttacttt agatatgccg agctatttgc catttatgac
    12481 ctatgctgat aatcgtgaat tgcgaaaaga attagctatt gctgctggta aaaaagggtt
    12541 tcaaaacaac gaatacgata atcaaaacaa cgttaaagac attgttcgtt tacgtcatga
    12601 acgcgctaat ttattaggtt atgcttctca tgcgcatttt gttttggaag aacgaatggc
    12661 tcaaaatcct gagaaagtta tttccttttt gaatgatatg ctggaaaaag caaaaccggc
    12721 tgctcaaaaa caatttgaag aattgactgc atttgcaaaa gaactagatg gaattgatca
    12781 tttggagaaa tgggatagcg cttattattc cgaaaaactg aagcaaaaac tattcaattt
    12841 ggatgatgaa aaactgaagc catatttcaa attagaaaat gtactgaatg gtgcttttac
    12901 cattgct
//
LOCUS       DAOOBH010000064        12749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_6345_length_12749_cov_7.463605, whole genome shotgun sequence.
ACCESSION   DAOOBH010000064 DAOOBH010000000
VERSION     DAOOBH010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12749
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6345_length_12749_cov_7.463605"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /gene="metG"
                     /locus_tag="PK218_07545"
     CDS             complement(<1..1438)
                     /gene="metG"
                     /locus_tag="PK218_07545"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPW98397.1"
                     /translation="MLENPKRYTITAALPYTNGPIHIGHLAGVYVPSDIYARYLRLQG
                     KDVAFMCGSDEHGVAISMKAKKEGITPQQVIDKYDAIIRKSFEDFGISFDNYSRTSAK
                     IHHDTAQEFFKKLYEKGDFIEEVTEQLYDAKADQFLADRFVTGTCPKCGNPEAYGDQC
                     EKCGSTLNATDLINPKSTITGETPIVKETKHWFLPLDRYQDFLEQWILVDHAKDWKVN
                     VLGQVKSWLDDGLKPRAVTRDLDWGIDVPVDGAEGKKLYVWFDAPIGYISATKEWAAH
                     EGKNWEDYWKKEDTKLVHFIGKDNIVFHCIIFPAMLKAEGSFILPDNVPANEFLNLEG
                     NKLSTSKNWAVWLHEYLIDFPEKQDVLRYALTANAPETKDNDFTWKDFQARNNNELAA
                     VYGNFINRVVVLTNKYYNGIIPSPNEFTEVDEQTLAELKAYPAVISSSIERYRFREAQ
                     NELMNVARLGNKYLADEEPWKMVKENPER"
     gene            1593..2489
                     /locus_tag="PK218_07550"
     CDS             1593..2489
                     /locus_tag="PK218_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPW98398.1"
                     /translation="MITPPFLQKGDTVAIVSTARKNLEDNLKPSIDLLESWGLKVKIG
                     STIGLDYHQLAGTDAQRAADFQEQMDNPNIKAIWCVRGGYGTVRIIDLLDFTKFKQNP
                     KWIIGFSDVTVLHSHLNRMGIQSIHGTMPISIPRATTEAKESLRKALFGEPLSYQFDC
                     DILNHVGTAKGELVGGNLSILYSLLGSESAIDCTDKILFIEDLDEYLYHIDRMMLNLK
                     RNGCLESLKGIIVGGMTKMNDNEIPWGKNAFEIIEEVTKDLKIPIIYNFPAGHIQDNR
                     ALIMGKQITMEVTAEESKVTFE"
     gene            2489..2848
                     /locus_tag="PK218_07555"
     CDS             2489..2848
                     /locus_tag="PK218_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPW98399.1"
                     /translation="MAQHNELGKFGEEMAVEYLQKNGYEILETNWTFQKAEIDIIAQK
                     ENILAIVEVKTRSSIDFGLPQDFVKPKKIQLLVKAVNEYVIRNDLDLNVRFDIIAIYK
                     ENKEYKIEHLMDAFYIF"
     gene            2929..5103
                     /locus_tag="PK218_07560"
     CDS             2929..5103
                     /locus_tag="PK218_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HPW98400.1"
                     /translation="MKKTLLILLSFFCFTIGFGQNKISQKIQKLQKENTRFIPFSLFT
                     VDNSISKTDVDKVVTQAALAKANISKINQIASGQFEFLELEIPYLNQNLKIQLYRVDP
                     FVEDFKVDTDKNKNIPYQKGVYYRGIINGNLQSVSAFNFFNGEFNGIFSSEQFGNVVV
                     GKIDKPNNQNDYIVYSDVNLKILNDFECHVKEDDTPPTASTLSREVNTEKCVTFYFEI
                     DNNLFQANSSNTTITTNWMTSVFNNVQTLFDNDGITTALKSIYIWTNPDPYDGIGNAS
                     SDYLQAFAVNRPVFNGDVGMLVGIDPGGLGGVAYLNQVCDDSNYAYSDLNGTSFSSVP
                     NYSWTIQVITHEFGHSLGSPHTHRCAWNGNNTSIDGCGTQAGYPEGSCATGPIPSSSV
                     KGTIMSYCHLISGVGINFANGFGPQPANLIVNTVNSKSCLSTDCINTCVNTVTNIQAS
                     NVTESTATITWEDINENISSWQISVTAFNSVPVWNTVSTTTFSVSGLLQNKYYTIAIR
                     PVCIGISTTIRRKIFSTAGNYCGGMVFTDTGNIADDYSNMESFTRTFIPNVPNRKIVV
                     NFTEFSLEDTYDFLYIYDGPDDTYPELSFADGFTGTNSPGLIVSTALDGSLTFKFTSD
                     QALTDSGWIATVSCEEHLGVISNDFIDFTYYPNPTKNNITLKSNTNITELEVFNIEGR
                     KLFTEKVNTLETNVDLSQFASGTYFFKVKFNDIEKNFKILKL"
     gene            complement(5158..8517)
                     /gene="mfd"
                     /locus_tag="PK218_07565"
     CDS             complement(5158..8517)
                     /gene="mfd"
                     /locus_tag="PK218_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPW98401.1"
                     /translation="MKPSSLFTTYEHSAKVTQLADALSEAANKVHCKGLMNSATSIVI
                     QSVFKKADKPFLLLFNEKEEAAYYLNDLEQMLGQNDVLFYPSSYRRPYQIEETDNANV
                     LLRSEVLNRINSRKKPAIIVSYPEALFEKVVTRKELEKNTLKISVGDQVSIDFINEVL
                     FEYDFKRVDFITEPGEFSVRGGIVDVFSFSNDNPYRIEFFGNEVDSIRTFDVESQLSV
                     EKQNKITIIPNLENKFIKENRESFLDYISDKTVLWIENTEILVAQLDKLFEKATETFD
                     KLSKDIKHASPEQLFMNSQGFLKQALNFSVVELSNTPLFKASKSFEFHLQPQPSFNKQ
                     FDLLLNNLSENHFNGITNFLFCSNDSQAKRFHDIFESIDEENHEDVRKQYHTVVQPMY
                     QGFIDEEHQVACYTDHQIFERYHRFTIKNSYSKKQTITLKELNSLTVGDYVTHIDHGI
                     GKFGGLQKIQVEGKTQEAIKLVYADNDLVYVSIHSLHKISKFNGKDGTPPKIYKLGSN
                     AWKVLKQKTKARVKHIAFNLIQLYAKRRLEKGFAFAPDSYLQKELESSFIYEDTPDQI
                     TATADVKADMEKERPMDRLVCGDVGFGKTEVAIRAAFKAVDNSKQVAILVPTTILAYQ
                     HYRTFSERLKDMPVNIGYVNRFRTAKQKKETLDALAEGKLDIIIGTHQLVNKNVKFKN
                     LGLLIVDEEQKFGVNVKDKLKTIAANVDTLTLTATPIPRTLQFSLMAARDLSVITTPP
                     PNRYPIETQVVGFNEEIIRDAITYEIQRNGQVFFINNRIENIKEIAGMIQRLVPDARV
                     GIGHGQMEGTKLEEIMLSFMNGEFDVLVATTIIESGLDVPNANTIFINNANNFGLSDL
                     HQMRGRVGRSNKKAFCYFVCPPYSAMTDDARKRIQALEQFNELGSGFNIAMKDLEIRG
                     AGDLLGGEQSGFINDIGFETYQKIMNEAIEELKENEFKDLYEEENTKETKTFVKELQI
                     DTDFELLFPDDYINSVNERLSLYNELGNCKNEEELTAFENKLIDRFGPLPKEAKALLN
                     SIKIKWIATKIGIEKLVMKQGKMVGYFVSDQQSAYYASRSFTKVLQFVQQHGNICKMK
                     EKETKNGLRLLLTFENVKSVNKALEFMELLK"
     gene            complement(8520..9185)
                     /locus_tag="PK218_07570"
     CDS             complement(8520..9185)
                     /locus_tag="PK218_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98402.1"
                     /translation="MKISARLLLFIISFLFLASCKDEVEQRNIEQQKELQKREEMFIK
                     VDKNWNFNIVTINAPSQKLVHDWTEWRNFLNEINQKPKSSIGAFQKKARALSKRALEL
                     NNNIPEKYAKPEVKSRIAVLQTKINSLDLYINLQTILDKKIIKLIPEINQEIQSLQLQ
                     FGEIDQKSQIKMEDGEQDMIRMLDTTRAIPMTKPIPTTEKTTPEEKLRYLREKRNTLI
                     PTH"
     gene            9326..10264
                     /locus_tag="PK218_07575"
     CDS             9326..10264
                     /locus_tag="PK218_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HPW98403.1"
                     /translation="MNAKILIIGACGQIGTELTHKLREIYGIENVVASDIRKLNNDVV
                     NNGIFEVVNALDYNQIQHLVEEYQITDVYLMAALLSATAEKNPAFAWDLNMNSLFHVL
                     NLAKAGKIKKIFWPSSIAVFGPTTPSVNTPQYTIMEPTTVYGISKQTGERWCEYYNKQ
                     YGVDVRSIRYPGLISWTTEPGGGTTDYAVEIYHQALLKGKYKCFLSEDTALPMMYMDD
                     AIKATIEIMQADADKIKIRSSYNLSGVSFTPKEIAEEIKKHIPEFRITYKPDFRQKIA
                     DSWPASIDDTNAREDWQWKHQFDLATMTIEMLENLK"
     gene            complement(10543..11043)
                     /locus_tag="PK218_07580"
     CDS             complement(10543..11043)
                     /locus_tag="PK218_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW98404.1"
                     /translation="MKKYIFFLLILSACGSKKEIPLTDDQKIDQLLTNWHKAAAAANF
                     GNYFGAMTEDAIFIGTDATEYWNKTQFEAYAKPYFDKGKAWSFKSLKRNIYFSNDKKM
                     AWFDELLDTQMKICRGSGVLVNTDQGWKIKQYVLSMTVPNDDVEAVVKIKTPAEDLLI
                     RLLQNK"
     gene            11214..11894
                     /locus_tag="PK218_07585"
     CDS             11214..11894
                     /locus_tag="PK218_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPW98405.1"
                     /translation="MNTKKKEFVILTIMIMQFSFAQFKTVIGKTDYPFWIKVPEKTEA
                     QPLLLFLHGKSLSGTDINKVRKYGVLYAMDKGKEIPAVVVAPQLASGSWNPDKVLEVL
                     EYVKAHYNIDEKRIYVCGMSLGGYGTMHFAGKYPEKITAAVEICGGGNTADGCRLGTI
                     PTWLLHGDKDKLVKMSESEKVFDAIKGCNPKADTQFTVVKGGTHGSVERLFHEDAIYE
                     WMFSKVRE"
     gene            complement(11998..>12749)
                     /locus_tag="PK218_07590"
     CDS             complement(11998..>12749)
                     /locus_tag="PK218_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98406.1"
                     /translation="DNTEYASFRDLNIGTFSIGNLNYNYTSIPTTFTETVCNGVFPFS
                     SYCGFGVCIGNYYNFGYYVGATFVSYPLNSYQISYFQSDSNNDGLIDCYKSEFIISKT
                     LPNGNPIITGFRAVVQSAQGNYSSGNIVKTIDLIFDGTSWNYTTTNYLGNTRPFVDDV
                     CNSLNTSVNEIESNNIKLFPNPTNDIAHISTPNEIINQINVYDLLGRLLKTQNGNNMS
                     EQINIQELPNAIYILEINTEKAKHSVQVVKK"
BASE COUNT         4167 a         2185 c         2097 g         4300 t
ORIGIN      
        1 cacgttctgg attttctttt accattttcc atggttcttc gtcggctaaa tatttgtttc
       61 caagtcgagc tacattcatc aattcgtttt gtgcttcacg gaaacgataa cgttctatag
      121 aagaagaaat aactgctgga taggctttta attcggctaa agtttgttca tcaacttcgg
      181 taaattcatt aggtgatgga ataattccgt tgtaatattt attggttaaa accaccacac
      241 gattgataaa atttccataa acagcagcca attcattatt atttctcgcc tgaaaatctt
      301 tccaagtaaa atcattgtct ttagtttctg gtgcatttgc cgtcaaagca taacgcaaca
      361 catcttgttt ttctggaaaa tcaattaaat attcgtgcag ccaaaccgcc caatttttag
      421 aggttgacaa tttatttcct tctaaattta aaaattcatt cgccggaaca ttatcaggaa
      481 gaataaaact tccttcggct tttagcatcg ctgggaaaat aatgcaatgg aaaacaatat
      541 tgtcttttcc gatgaagtga acgagttttg tatcttcttt tttccaataa tcttcccaat
      601 tttttccttc gtgtgctgcc cattctttag tagcagaaat gtatccaatg ggtgcatcaa
      661 accaaacgta taattttttt ccttcggcac catcaaccgg aacatcaatt ccccaatcta
      721 aatcacgagt aacagcacga ggttttaaac catcgtctaa ccatgatttt acttgaccca
      781 aaacatttac tttccaatct ttggcatggt caaccaaaat ccattgttcc aaaaaatctt
      841 gatatctatc taaaggtaag aaccagtgtt tggtttcttt tacaattgga gtttcacctg
      901 ttatggttga ttttggattt attaaatcgg ttgcatttag agtcgaacca catttttcac
      961 attgatcgcc atacgcttca ggatttccgc attttggaca agttcctgta acaaatctat
     1021 cagctagaaa ttggtcggct ttggcatcgt acaattgctc ggttacttct tcaataaaat
     1081 cacctttttc ataaagcttt ttgaaaaact cttgcgccgt atcgtgatga attttagcag
     1141 aagttcgaga ataattatca aacgaaattc cgaaatcttc aaaagattta cgaataatcg
     1201 cgtcgtattt atcgataacc tgttgaggag taatgccttc ttttttggct ttcatcgaaa
     1261 tggcaacgcc atgttcgtca cttccacaca taaaagcaac atcttttcct tgcaatcgca
     1321 aatagcgcgc ataaatatca cttggaacat aaacacctgc taaatgacca atatgaattg
     1381 gaccattggt ataaggcaaa gctgccgtaa ttgtatatcg ttttggattt tctaacataa
     1441 tctatcaaat agaatgcaaa aataagtttt ttagctacga attcatcaca ttagtgttga
     1501 tttgtttatt tttgaaaaag caatttcaat acatgcgaat actattatta ttgattttta
     1561 gtttaacact ttcttcttat tcccaaaaaa agatgattac acctccgttt ctacaaaaag
     1621 gcgataccgt tgccatcgtt tctacagctc gaaaaaatct tgaagataat ttaaaaccat
     1681 caattgattt attggaatct tggggattga aagttaaaat tggttcaaca attggattgg
     1741 attatcatca attggcagga actgatgcac aacgtgcagc cgattttcaa gaacaaatgg
     1801 ataatccaaa cattaaagca atttggtgtg ttcgtggcgg atatggaacg gttcgtataa
     1861 ttgatttgct tgattttaca aaattcaaac aaaacccaaa atggattatt gggtttagtg
     1921 atgttacggt tttacacagt catttgaacc gaatgggaat tcaatcgatt cacggaacaa
     1981 tgccaatatc tattcctaga gctacgacag aagccaaaga aagtttacgg aaagctttgt
     2041 ttggcgaacc gctaagctat caatttgatt gtgacatttt aaatcatgtc ggaactgcca
     2101 aaggtgaatt ggttggagga aacttatcca ttttatacag tttattaggt tcagaatcgg
     2161 caattgattg tacagataaa attcttttta tagaagattt ggatgaatat ttatatcata
     2221 ttgaccgaat gatgcttaac ttaaaacgca atggttgttt ggaaagttta aaaggaatta
     2281 ttgttggtgg aatgacaaaa atgaatgata atgaaattcc ttgggggaaa aatgctttcg
     2341 aaatcattga agaagttacc aaagatttaa aaattccgat tatttataat ttcccagctg
     2401 gtcatattca ggataatcgt gctttgatta tgggaaaaca aattaccatg gaagttaccg
     2461 ccgaggaaag caaagttact tttgaataat ggctcagcac aatgaattag gaaaatttgg
     2521 ggaagaaatg gctgtagaat atctgcaaaa gaacggctat gaaattttgg aaactaattg
     2581 gacgtttcaa aaagcagaaa ttgatattat tgctcaaaaa gaaaatattc ttgctatcgt
     2641 tgaagtaaaa acacgttcta gtattgattt tggattacca caagattttg tcaaaccaaa
     2701 aaaaatccaa ctactcgtaa aagccgtaaa tgaatatgta attcgtaatg acttagattt
     2761 aaatgtgagg tttgatatta ttgcgattta taaagagaat aaagaatata aaatagaaca
     2821 tttaatggat gctttttata ttttttagag taaacaaatg ataaaatgat gtaaaattta
     2881 tattatttta gttgattttc aataacttta cgctttacaa aaaatactat gaaaaaaacc
     2941 ctactaattc tcttaagctt cttttgcttt acgattggat ttggtcaaaa caaaatcagc
     3001 caaaaaattc aaaagctaca aaaagaaaac acacgtttta tacccttttc gctatttact
     3061 gtggacaatt caatttctaa aacagatgtt gataaagtcg taactcaagc tgctttggca
     3121 aaagcgaata ttagcaaaat caaccaaatt gcatcgggac aatttgaatt tctagaactt
     3181 gaaatcccat atttaaacca aaatttaaaa attcaattgt atcgtgttga tccttttgtt
     3241 gaagatttta aagtagatac cgataaaaat aaaaacatac cttatcaaaa aggagtgtat
     3301 tatcgtggaa tcattaacgg aaatttacaa tccgtttcag ctttcaattt ctttaatgga
     3361 gaatttaacg gtattttttc ttcagaacaa tttggaaatg ttgtagtagg taaaattgac
     3421 aaaccaaaca atcaaaacga ttatattgta tattctgatg ttaatttgaa aatcttgaat
     3481 gattttgaat gtcatgtaaa agaagatgat acgccaccaa cggcatcaac actaagcaga
     3541 gaagtgaata ctgaaaaatg tgttactttc tattttgaaa ttgataataa tctatttcaa
     3601 gctaatagca gcaatactac aattactact aattggatga cttctgtttt taacaatgtg
     3661 caaacattat ttgataatga cggaataaca acagcattaa aatcaattta tatttggaca
     3721 aacccagatc cctatgacgg cattggcaat gcttcaagtg attatttaca agcttttgca
     3781 gtaaacagac ctgtttttaa tggcgatgtt ggaatgcttg taggaattga cccaggtgga
     3841 ttaggcggtg ttgcttatct aaatcaggtt tgcgatgatt ccaattatgc ttattccgat
     3901 ttgaatggaa cttctttttc ctcggttccc aattattctt ggactattca agtgattaca
     3961 catgaatttg gacattcatt aggttcgccc catacacatc gttgcgcatg gaatggtaat
     4021 aatacttcta ttgatggatg tggtacgcaa gcgggatatc ccgaaggtag ttgcgctact
     4081 ggtccaattc cttcatcatc tgtaaaagga acaataatga gttattgcca tttaatttca
     4141 ggtgttggaa tcaattttgc caatggtttt gggccacaac cagcaaatct tattgtaaac
     4201 actgttaata gcaaatcatg tttaagtacg gattgtatta atacttgtgt gaatacggta
     4261 acaaacattc aagcatcaaa tgttaccgaa agtaccgcta ccattacttg ggaagatatt
     4321 aatgaaaata tttcttcatg gcaaatctcg gtgactgctt ttaactctgt gccagtttgg
     4381 aataccgtta gtacaacgac tttttcagtg tcaggattat tgcaaaataa atattatact
     4441 atcgctattc gacctgtatg tattgggatt tcaaccacaa ttagaaggaa aatattttcc
     4501 actgctggaa attattgtgg cggaatggta tttactgata ctggtaatat cgctgatgat
     4561 tattctaaca tggaatcttt tacaagaact tttattccaa atgtacccaa tagaaaaata
     4621 gtggttaatt ttactgagtt ttctcttgaa gacacttatg atttcttata tatatatgat
     4681 ggaccagatg atacatatcc tgaattaagt tttgcagatg gatttacagg aacaaattct
     4741 ccaggtttaa ttgtttcaac ggctttggat ggttcgctta cattcaaatt cacttcagac
     4801 caagcattga cagactcagg atggatagct actgtaagtt gtgaagaaca cttaggtgtt
     4861 atttcaaatg attttattga ttttacctat tatccaaatc caacaaaaaa taatattact
     4921 ttaaaatcca ataccaacat tacagaactt gaagtgttta atattgaagg aagaaaatta
     4981 tttaccgaaa aagtaaatac tttagaaaca aatgttgatt tatcacagtt tgcttcggga
     5041 acgtatttct ttaaagtgaa atttaatgac attgaaaaaa atttcaaaat attgaaatta
     5101 taaaaattta cccaacagat tacaattgga aaggctactc gaaagagtgg ctttttctta
     5161 tttcaacaac tccataaact ccaacgcttt gttgacggat tttacatttt caaaagttaa
     5221 gagcaagcgc aaaccatttt tggtttcttt ttctttcatc ttgcaaatgt ttccgtgttg
     5281 ttgtacaaac tgcaatactt tggtaaacga tctcgaggca taatacgccg attgctgatc
     5341 agaaacaaaa taaccaacca tcttgccttg tttcattacc agtttctcaa tacctatttt
     5401 agtagcaatc catttaatct tgatgctgtt taatagtgct ttggcttctt taggcaaagg
     5461 tccaaaacgg tcaatcagct tattttcaaa agcagttaat tcttcttcgt ttttacaatt
     5521 ccctagttcg ttatacaaac tcaacctttc attcacgctg ttgatataat catctgggaa
     5581 caacaattca aagtcagtat ctatttgtaa ttctttgaca aaggtttttg tttctttagt
     5641 attttcttct tcgtacaagt ccttgaattc gttttctttc aattcctcaa tcgcttcatt
     5701 catgattttt tgataggttt caaaaccaat atcattaata aaaccacttt gttcgccacc
     5761 aagcaaatct ccagcaccac gaatttccaa atccttcatg gcaatgttaa agccgcttcc
     5821 taattcgtta aactgttcta atgcttgaat acgttttcga gcatcatctg tcattgctga
     5881 atatggagga caaacaaaat aacaaaatgc tttcttatta ctacgtccaa cgcgtccacg
     5941 catctgatgc aaatcagaca gtccaaaatt attagcatta ttaataaaaa tggtgttcgc
     6001 atttggtaca tctaatccac tttcgataat ggttgtcgct accaaaacat caaattcacc
     6061 gttcataaaa ctcaacataa tttcttccaa cttggtgcct tccatttgcc cgtgaccaat
     6121 accaactcgt gcatcaggaa ccaaacgttg aatcattcct gctatttctt tgatgttctc
     6181 tattctatta ttgatgaaaa acacttgacc atttcgttga atttcataag taatcgcgtc
     6241 acgaataatc tcttcattaa aaccaaccac ttgtgtttct ataggataac ggtttggcgg
     6301 tggtgtagtg attactgaca aatctcgtgc tgccattaac gaaaattgta aagttctcgg
     6361 aattggagtt gcggttaatg ttaaagtatc cacattggcg gcaatggttt ttagtttgtc
     6421 ttttacattt acaccaaact tttgttcttc atcaacaatc aacaatccca aatttttaaa
     6481 cttcacattt ttattcacca attgatgcgt gccaatgata atgtctaatt ttccttcggc
     6541 taatgcgtct aaggtttctt tcttttgttt tgccgttcta aatcggttta cataaccaat
     6601 gttaactggc atatctttta aacgttcgct gaacgttcgg taatgttgat aggctaaaat
     6661 tgttgtaggc accaaaattg caacttgctt gctattgtca actgctttaa atgctgcacg
     6721 aatagccact tctgtctttc caaaacctac atcaccacaa accaatcgat ccattggtct
     6781 ttccttttcc atgtcggctt ttacgtcagc agttgccgtt atttgatctg gcgtatcttc
     6841 atagatgaac gaactttcca attctttttg caaataacta tccggtgcaa aagcaaatcc
     6901 tttttccaac cttcgcttgg catacaactg aattaagttg aatgcaatgt gttttactct
     6961 agctttggtt ttctgtttta atactttcca agcatttgaa cccagtttat aaatctttgg
     7021 cggtgtgcca tcttttccgt taaacttcga tattttgtgt agcgaatgaa tgctcacata
     7081 caccaaatca ttatcggcat acaccaactt aatcgcttct tgcgttttgc cttctacctg
     7141 aattttctgc aagccaccaa acttaccaat tccgtggtca atatgcgtaa cataatcgcc
     7201 aacggttagt gaattcagtt cttttaacgt aatagtttgt tttttcgaat aactattttt
     7261 aatcgtaaaa cgatggtaac gttcaaaaat ctgatggtca gtataacatg ctacttgatg
     7321 ttcttcatct ataaaacctt gatacatcgg ttgcacaacg gtatgatact gtttccgaac
     7381 atcttcatga ttttcttcat caatgctttc aaaaatatcg tgaaaacgtt tggcttgcga
     7441 atcattagag caaaacaaaa agttagtaat gccattaaaa tgattctcgc tcaaattgtt
     7501 caacaacaaa tcaaactgtt tgttgaacga aggttgcggt tgcaaatgaa attcaaacga
     7561 tttgcttgct ttaaacaagg gtgtatttga caattcaaca actgaaaaat tcaaggcttg
     7621 ttttaaaaaa ccttgactgt tcataaacaa ttgttcgggc gaagcatgtt taatatcttt
     7681 cgaaagctta tcaaacgttt cagtcgcttt ctcaaaaagt ttatctaatt gcgccacgag
     7741 aatttcagtg ttttcaatcc acaaaaccgt tttgtcactg atgtaatcca aaaaactttc
     7801 acggttttcc ttaataaact tattctccaa gtttggaata atagtaatct tattttgttt
     7861 ctcaaccgac agttgacttt ctacatcaaa cgttcggata ctatccactt cattgccaaa
     7921 aaactctata cgatacggat tgtcattaga gaatgaaaaa acatccacaa ttccaccacg
     7981 aacagagaat tctcctggtt ccgtaataaa atctacgcgt ttaaagtcat actcaaaaag
     8041 cacttcattg ataaaatcta tcgaaacttg gtcgccaaca ctaatcttga gagtgttttt
     8101 ttccaattct tttcgagtaa ctactttttc aaacaaagct tcgggatacg aaacaataat
     8161 agcaggtttc tttctcgaat tgattctatt caaaacctcc gaacgcaaca aaacattagc
     8221 attatcggtt tcttcaattt gataaggtct gcgataggaa cttggataaa acaacacatc
     8281 gttttgaccc aacatttgct ccaaatcatt caaataataa gcagcttctt ctttttcgtt
     8341 gaataacaac aaaaatggtt tatcggcttt cttaaaaaca ctttgtataa caattgacgt
     8401 tgccgaattc atcaagcctt tgcaatgcac tttgtttgct gcttctgaaa gagcatctgc
     8461 caactgtgtt actttagctg aatgttcata ggttgtaaaa agtgatgaag gtttcaaact
     8521 tagtgtgtag gaattagtgt gttgcgtttt tctctcaaat accttaattt ctcttctggt
     8581 gtagtctttt ctgtcgtagg aataggtttt gtcattggga tagcacgagt cgtatcaagc
     8641 attctaatca tatcttgttc gccatcttcc attttaattt gacttttttg gtctatttcg
     8701 ccaaattgta attgcaacga ttgaatttct tgattgattt caggaataag tttgataatc
     8761 tttttatcca atatagtttg aagattgatg taaagatcca acgagttaat tttagtttgc
     8821 aaaacagcaa ttctactctt aacttcaggc ttagcatact tttcgggtat attattattt
     8881 agttccaatg ctctttttga cagtgctctt gctttttttt gaaaggcacc aatactactt
     8941 tttggttttt gattaatttc attcaagaag ttgcgccatt ccgtccaatc atgcacaagt
     9001 ttttgagaag gagcattgat agtaacaata ttaaaattcc aatttttatc aaccttaata
     9061 aacatttctt ctcgtttttg taactctttt tgttgttcaa tgttgcgctg ttcgacttca
     9121 tctttacaag aagctaaaaa caaaaaagaa attataaaca gtagtaaccg agccgatatt
     9181 ttcataaaaa agattagaaa tacaaaagta caaaggtaat tagaaatacc acagacttaa
     9241 tgttataaga atgataaaaa actacataaa agtaaaacac taaaccttgc gaaaactgta
     9301 tctttgtttt tttatataaa acaatatgaa tgcaaaaata ttaattattg gtgcttgtgg
     9361 acaaataggc acagagttaa cacataaatt aagagaaatt tacggcatag aaaacgttgt
     9421 ggcttccgat attagaaagc ttaacaacga tgtagtcaac aatgggattt ttgaagtggt
     9481 aaatgcttta gattacaatc aaattcaaca tttagtagaa gaatatcaaa taacggatgt
     9541 ttatttaatg gcggctttgt tatcggcaac tgctgaaaaa aacccagcct ttgcgtggga
     9601 tttaaacatg aattctttat ttcatgtatt aaatttagcc aaagcaggaa aaatcaagaa
     9661 aatattctgg ccatcaagca ttgcagtttt tgggccaaca acacctagtg tcaatacgcc
     9721 acaatatacc attatggaac caacaaccgt ctatggaatt agtaaacaaa caggtgaacg
     9781 ttggtgcgaa tactataaca aacaatacgg tgttgatgtt agaagcattc gctacccagg
     9841 gttaattagt tggactaccg aaccaggcgg agggacaacc gattatgctg ttgaaattta
     9901 ccatcaagca ttgttaaaag gcaaatacaa atgtttcctt tccgaagata cggctttgcc
     9961 aatgatgtat atggacgacg ctataaaagc taccattgaa atcatgcaag ccgatgcaga
    10021 taaaatcaaa atccgttctt cctataattt atcgggagtt agtttcacgc caaaagagat
    10081 tgctgaagaa atcaaaaaac acatccccga attcagaatc acttacaaac ccgatttccg
    10141 ccaaaagata gcagacagtt ggcctgcgtc tattgatgat acaaatgcta gagaagattg
    10201 gcaatggaaa catcaatttg atttagcaac aatgaccatc gaaatgttag agaatctaaa
    10261 ataaacaaaa agcgttgtac aacattaagt acaacgcttt ttttcaacca aaaccaatct
    10321 aaccaaacct ttttttaatt gaaataatct ttcagaaatt aaaaggcaat gcataatcaa
    10381 aaaggatagt aatgtaacct aaacaaagga atagtgatga cactaacgta agtacaagtg
    10441 taaaaaacaa tccgtctatt tttttattga tatccatatt cttgcctttc ttgttatcat
    10501 atacgagaaa gaaaagaatg cggttttatt taagtaaaaa aattatttat tttgtaaaag
    10561 ccttattaat aagtcttcgg ctggtgtttt tatttttaca acagcttcta catcatcatt
    10621 tggaacagtc attgagagca catattgttt tattttccaa ccctgatcgg tattgaccaa
    10681 tacgcccgaa ccacgacaaa tcttcatttg cgtatccaaa agttcatcaa accaagccat
    10741 ttttttgtca ttactaaagt agatgttgcg ttttagcgat ttaaaactcc atgcttttcc
    10801 tttatcaaaa tagggttttg cataagcttc aaattgagtt ttgttccaat attcagtagc
    10861 atcagtgcca ataaatatag cgtcttcggt cattgcgcca aagtaattgc caaaattagc
    10921 tgctgctgct gctttgtgcc aattggttaa aagttgatct attttttggt catctgttaa
    10981 tggaatttct tttttagagc cacaagccga aaggataagt aaaaagaaaa tatatttttt
    11041 catgatttaa aagtttcttt aaagatacta tattatgatt aacattacta tgaacattga
    11101 ttcctttttg aaatttacaa aattattaat agtgctattt ttaatttatc atagtgaata
    11161 gtaaaattaa tctttgctta ggattatctt tgcctcttat taaaaaagta aaaatgaata
    11221 caaagaaaaa ggaatttgtg attttaacga taatgataat gcaattcagt tttgcacaat
    11281 ttaaaaccgt tatcggaaaa acggattatc ctttttggat taaagttcct gaaaaaacag
    11341 aagcacaacc attattgtta tttctacatg gaaaaagcct ttcgggcacc gacattaata
    11401 aagtacggaa gtatggtgtt ctgtatgcaa tggataaagg aaaagaaatt cctgcggtag
    11461 ttgttgcgcc acaactggct agtggttctt ggaatcctga taaggttttg gaagttttgg
    11521 agtatgtgaa agcacattat aatattgatg aaaagcgtat ttatgtttgt gggatgagtt
    11581 tgggtggtta tggcacgatg cattttgcgg ggaaatatcc agagaagatt acggctgctg
    11641 ttgaaatttg tggcggaggg aatacggcag atggttgccg actaggaacg atacctactt
    11701 ggttgttaca tggtgataag gataaacttg tgaaaatgag tgaatcggag aaggtgtttg
    11761 atgctataaa gggctgcaac ccaaaagcag atactcagtt tacggttgtg aaaggtggca
    11821 ctcatggtag cgtggaacgc ttgtttcacg aggatgctat ttatgaatgg atgttttcga
    11881 aggttaggga gtagttttaa gtttcaggct tcaggtttcc ttagaaagta gcttagctcg
    11941 tgttatgaaa aaaactccaa aaacagtatg cttttggagt ttgaaaaact aaaactatta
    12001 tttcttaacc acttgcacac tatgttttgc tttttctgta tttatttcta gtatatatat
    12061 agcatttggc aactcttgaa tattgatttg ttcactcatg ttattaccgt tttgtgtttt
    12121 tagtaaacga ccaagtaaat cataaacatt gatttggttt atgatttcat ttggagttga
    12181 gatatgtgca atatcattcg ttgggttagg aaataatttt atgttattac tttcaatttc
    12241 atttacagaa gtgttcaatg aattgcaaac atcatctaca aatggacgag tattaccaag
    12301 atagtttgtt gttgtataat tccatgaagt accatcaaat attaaatcta tcgttttaac
    12361 tatattccct gaactgtaat taccctgagc agattgaact actgctctaa agccagtaat
    12421 aattgggttt ccgtttggta aagtttttga tattataaat tcacttttat aacaatctat
    12481 caaaccatca ttattactat ccgattgaaa atatgatatt tgataactgt ttaatggata
    12541 gctaacaaat gtagcaccaa catagtatcc aaaattgtaa taatttccaa tacaaacacc
    12601 aaaaccacaa taagacgaaa atggaaaaac accattgcaa acggtttcag taaatgtagt
    12661 tggaattgat gtatagttat aatttaaatt tccaatacta aatgtgccaa tgtttaaatc
    12721 tctaaaactc gcatactctg tattgtctt
//
LOCUS       DAOOBH010000065        12416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_6536_length_12416_cov_4.614432, whole genome shotgun sequence.
ACCESSION   DAOOBH010000065 DAOOBH010000000
VERSION     DAOOBH010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12416
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6536_length_12416_cov_4.614432"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK218_07595"
     CDS             complement(<1..396)
                     /locus_tag="PK218_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98407.1"
                     /translation="MFKKIISSSIFFVFLSCASESNTSNNNQTIVCDCPSGNITVNSN
                     DLIGDWKLNKRTILDVTGTTVTEIQDLTTLNPCNPDSFHLEVTLDANNNKIMSVPVGG
                     LATPALSNCGNILNIEFPVVYYENGGSCLK"
     gene            complement(406..1011)
                     /locus_tag="PK218_07600"
     CDS             complement(406..1011)
                     /locus_tag="PK218_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98408.1"
                     /translation="MKTTKIIYALTLSTILISCSTKQKDSETELSTNKKPVSEKKVET
                     STKEDSLKIKGDYDGDGIFEYASIKAPKQIENDACECEGGECIAYIVFSDSKIPQIKI
                     NGDIGGYLENVSDLNEDGKDELLYQQGWCTSTWRTWTLWTLKSDKWIYAIEPFEIRGD
                     IEENEKIVTKDLKKKGNVIVKYSSQKDENTGEIVTKSVKIK"
     gene            complement(1543..2037)
                     /locus_tag="PK218_07605"
     CDS             complement(1543..2037)
                     /locus_tag="PK218_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98409.1"
                     /translation="MPFANFDNRHFDAAEKTAVNNALSSLEDALKEKQANLSAEERQK
                     YGSVNEQNKLIINKVKDYHDTQPALSSPDIDWNEFNDDHDTRNFLQSTILRLQGLVIG
                     MENAKILHDYDNYSAALTDYDYSKYKAGTQSIGFQPKVDELAQFFSGRPTGSTAAKPT
                     EEEA"
     gene            complement(2090..2317)
                     /locus_tag="PK218_07610"
     CDS             complement(2090..2317)
                     /locus_tag="PK218_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98410.1"
                     /translation="MCWNTLRNNCSQLIDSYLFKESKPLNRKLLIQLTADEFPEIPRL
                     RIAFAVDKYLESQPQPITSSGFVHYMQHYLR"
     gene            complement(2387..2509)
                     /locus_tag="PK218_07615"
     CDS             complement(2387..2509)
                     /locus_tag="PK218_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98411.1"
                     /translation="MKQLLMNDYTLMLESDKKAKNIITIVVLFAIFLIVCDSIY"
     gene            2881..3381
                     /locus_tag="PK218_07620"
     CDS             2881..3381
                     /locus_tag="PK218_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98412.1"
                     /translation="MSTKKLQLGLVEEMVSNYKNNQLSSILSSTAHPMSFDAQSVWLD
                     LNTLKDFLKTIEEETALHPEYELKNFGVRFYYAAYPKKDKWDQQGYEDLAFMLTDPIA
                     KDYEKLHTVIAIPTTEIDGINQDFDPFDTKTYDGTKPTNTALAIMAENHGVLCPPPFS
                     GSGVWF"
     gene            3410..4075
                     /locus_tag="PK218_07625"
     CDS             3410..4075
                     /locus_tag="PK218_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98413.1"
                     /translation="MIVLFKILIKSLLYFELLPVIFGILYFKKFKDTYWKYFIYYLGF
                     IFIAGALHNWIIQKYFNYLGDDFGNYFLSYFVIPIEFLFFYWLYAYKSLNQKKLYWIF
                     SILYLLSFAFHALIKETISSIYSFNYVVGAFFLGILVFLEYMKQIKSDDIIKFKDNMM
                     FYVNLGVCMLYIGTLPFFSFFGILLKNMDIYMNYYTIFLIVNHLMYLLFTAAIIWGKP
                     NTY"
     gene            4051..4830
                     /locus_tag="PK218_07630"
     CDS             4051..4830
                     /locus_tag="PK218_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPW98414.1"
                     /translation="MGKTEYLLITIVFNLFFLLFMVAIITYMLQYKKKKKESEMLLEF
                     EKSKHQRELLASQLEMQQQTMQEIGREIHDNIGQKLTLASLYIQGNMYENKTFESNEN
                     LQNISNIINESLSDLRQLSKTLTDDAIAKRNIVALLQLECEKVSRIKKCLLYHYDDID
                     KIEISYHLKSMLLRIAQEFIQNSLKHSECEGINLHLSHAENFIVLKIMDDGKGFDVSN
                     PNTLGIGLQNMKKRTESVGGTFFLESSNTGTHLTLKFPIIL"
     gene            4888..5553
                     /locus_tag="PK218_07635"
     CDS             4888..5553
                     /locus_tag="PK218_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW98415.1"
                     /translation="MKHSIVIVDDHILIAKALGSIISNFRDFEVLYECGNGKELQDKF
                     KDKNNIPKIVLLDISMPIMDGYATSKWLKEAHPLVLILALSMQDDEQSLIKMIKNGSK
                     GYLLKNVAPTELETALRRIVNKGHYFPDWASDIVFANMFNKETIEEVVEYFEISDREK
                     EFLCLCTTEMSYKEMGKIMKCSPRTIEYYRNTLCKKLKVKSRIGLAVYAMKSGLVKDN
                     YSS"
     gene            complement(5541..5768)
                     /locus_tag="PK218_07640"
     CDS             complement(5541..5768)
                     /locus_tag="PK218_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98416.1"
                     /translation="MQLPKFVLADNSDFPDDIFIIHLDFPRFLINLKDDEVEFLEDLE
                     GEDETELESEMEHLITLAGEFYDKEMKNYEE"
     gene            complement(5772..7145)
                     /locus_tag="PK218_07645"
     CDS             complement(5772..7145)
                     /locus_tag="PK218_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPW98417.1"
                     /translation="MTLSNYTKEFKYNMQLAYPIILGMIGHTVVSIVDNVMVGKLGPT
                     ELAAVSLGNSFVFIAMSLGIGFSTAITPLIAEADGKKSIEEGRSVFHHGLYLCTILGI
                     VLFALIYFCKPLIAYMGQSDEVVTMAKPYLDIVGFSLIPLIVFQAYKQFADGLSETKY
                     SMWATILGNVVNVVINYFLIYGIWIFPKMGIVGAAIGTIASRFVMLGFMHYMMNLKTK
                     FHPYFKGFTLKEIKKEINIKIIRLGTPSAMQMLFEVVLFTAGIWLCGIIGVTSQAANQ
                     VALSIASLTFMFAMGLSVAAMIRVGNQKGKSDFITLRRVALSIFLLAILLEIVFALLF
                     VVFHEVIPTLFIKNNDVINMVANMEVVTIAANLLLVAAVFQISDGIQVVVLGALRGLQ
                     DVKVPMYMTFVAYWLIGFPISYYLGLYTELKAVGVWIGLLAGLSAAALFLYLRFNYLT
                     KRLITNH"
     gene            complement(7182..8054)
                     /locus_tag="PK218_07650"
     CDS             complement(7182..8054)
                     /locus_tag="PK218_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HPW98418.1"
                     /translation="MKTINPDDFKVISPIKLNNIPTELDMDTSKKQEETALDEIQVKL
                     SRKQDMMYAHNRYAFLICLQGMDTSGKDSLIREVFKEFNSRGVVVQSFKTPTSNELEH
                     DYLWRHYIALPEKGKFAVFNRTHYENVLVTRVHPEYILFENLPGIEKVEDITPQFWEN
                     RINQINNFEKHISQNGTKILKFYLHLSKEEQRKRLLKRLETPEDNWKFSTGDLKERAL
                     WDDYMKYYEEAINKTSTDYAPWYVVPADDKGVARYIVAKTIWQELDKLKDIAEPQLDP
                     KVKANFDEYRKQLE"
     gene            8280..10433
                     /locus_tag="PK218_07655"
     CDS             8280..10433
                     /locus_tag="PK218_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW98419.1"
                     /translation="MKIFYFFLFFCCSFITFAQTEISGTVVDQKNKPLAGANIYIDGT
                     YDGGTSDENGKFHFTTTETGSKNLVITMITYESFNEIIDVANCKDKTFILRESVNTLD
                     AVIITAGTFDAGEKARVSVLKPLDIVTTAGSNANIIAALQTLPGTQTVGEDGRLFVRG
                     GEADETQTFVDGIRVAQPYGATTGNVPTRGRFSPFLFSGMSFSTGGYSAEYGEALSSV
                     LLLNTTDEATEEKTDVSLMTVGLGLANTQKWKNNSITFNTAYIDLAPYQKLIPQNVSW
                     NKPFHSLSGETVYRHSFSNSIFKLYAAFDASAFDINQESINSPTPTRVDLSNNNFYLN
                     ASYKTNLGNQWKLFTGMAYGYGENKIDISTGRVFNVENSNHIKARVNKSLTDRIKITF
                     GGDYFITKFDEYFKSTDASRFDYGYDANIAAAYAETDIFFSRKLALKVGARISNNDYL
                     KETVFTPRLSMGYKVSKSGQFSLAYGMFSQAPRQEYLKFADYLSSEKANHYILNYQYN
                     KDRQTFRAEIYFKEYANLVKYDDANANFATVYNNKGQGYAKGIDIFWRDNKSIKNFEY
                     WISYSFIDTERDYKNYPTQATPSFVANQSLSVVGKWWIDKLKSQVSFTNSFATGRPYN
                     NPNESTFLGSKTKGYNNLSLSWAYLISQQKILYFSASNVLSTQNVFGYEYANTPNANG
                     MYDRRAIRPTADQFFFVGFFWTISENKKDNQLNNL"
     gene            complement(10428..11201)
                     /gene="mazG"
                     /locus_tag="PK218_07660"
     CDS             complement(10428..11201)
                     /gene="mazG"
                     /locus_tag="PK218_07660"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPW98420.1"
                     /translation="MNNRQEQLKAFNRLLDIMDELREKCPWDKKQTLESLRHLTIEET
                     YELGDAILDNNLPEIKKELGDLLLHIVFYAKIGSETNNFDIADVANSICDKLIDRHPH
                     IYGDVVAADEEEVKRNWEKLKLKEGKKSVLEGVPKSLPALVKASRIQDKVKGVGFDWE
                     EPHQVWDKVQEELQELQEEVKTNNQDKIEAEFGDVMFSMINYARFLNVNAEDALERTN
                     KKFIKRFMYLESKASELGKPLSDMTLAEMDVFWEEAKKL"
     gene            complement(12007..>12416)
                     /locus_tag="PK218_07665"
     CDS             complement(12007..>12416)
                     /locus_tag="PK218_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW98421.1"
                     /translation="NIVNSYTHKKKIEIVNTISQNHFVKFSPAYLESILINFTTNAIK
                     YAHPDRFPKLEFSLITKNNQKVLVIADNGVGIDLEKHGSALFGLYKTFHNKQETTGIG
                     LFITKYQIEAMKAKISVTSKVGFGTTFKIHFFD"
BASE COUNT         4003 a         2074 c         2009 g         4330 t
ORIGIN      
        1 ctttaaacaa cttcctccat tttcatagta aactacagga aattctatgt ttaaaatatt
       61 tccacaattt gatagcgcag gtgttgctaa tccaccaact ggaactgaca taattttgtt
      121 attattagca tctaatgtta cttctaaatg aaaagaatca ggattgcaag gattaagtgt
      181 tgttaaatct tgaatctctg taactgttgt tcctgttaca tctaaaattg tacgtttatt
      241 taatttccaa tcgccaatta agtcatttga atttactgta atgtttccac ttggacaatc
      301 acaaacaatg gtttgattgt tattacttgt attactttct gatgcacaac ttagaaatac
      361 aaagaaaata cttgaactaa tgattttttt aaacatattt tttcgttact taattttaac
      421 tgattttgtt actatttcgc cggtattttc atctttttgt gaagaatatt ttactatgac
      481 atttcctttt ttctttaaat ctttagttac aattttttca ttttcttcaa tgtctcctct
      541 aatttcaaat ggctcaattg cataaatcca tttgtcactt tttaatgtcc acaaagtcca
      601 agttcgccaa gtacttgtgc accatccttg ttgatataat aattcatctt tgccgtcttc
      661 atttaaatca ctaacattct caagataacc accaatatct ccattaattt tgatttgagg
      721 aatttttgag tctgaaaaca caatgtaagc aatacattct ccgccttcac attcgcaagc
      781 atcattttca atctgttttg gagcttttat cgaagcgtat tcaaaaattc catcgccgtc
      841 ataatcacct ttaattttta atgaatcttc ttttgttgaa gtctcgactt tcttctctga
      901 gacaggcttt ttattagttg aaagttctgt ttccgaatcc ttttgttttg tgctgcaact
      961 tatcaaaatt gttgataatg ttaaagcata aatgattttg gttgttttca tatgatttaa
     1021 taattaaatg ttcttttttt atgttcatgc ggtgtcttac cgctaactag tatatatcca
     1081 caagtttata caactttttt ctatttacaa aatgatttac ctactctctc agttttcggc
     1141 ttcgctgttt tagttactca agctactcac ttgctttttg cctcaaaaaa acaagtaagt
     1201 gttgagcttc tttatttgtg gttttctttt ttttataacc cttttaaaac cactttaaag
     1261 gggttgataa ctacttttaa tacgttggtt aacctatttt aagtggttca ttactggttt
     1321 tatgtggttg ataaacgtat attaagtggt ctataaccac ttaaaagtag ttcgaagcgt
     1381 attttaatag gttaaccaac gtattaaaag tcgtcttgaa cgtatattaa gtgattccag
     1441 acgtattaaa agtggttaga taacgtatta aaaccagttc agaacgtagt ataacaggtt
     1501 gtatagccta ttaaaactag ttctgaacgt atattaagtg gtttacgcct cttcttctgt
     1561 aggttttgca gcagtgctac ctgttggtct tcctgaaaaa aactgtgcta gttcgtcaac
     1621 ttttggctga aagcctattg attgtgttcc tgctttgtat tttgaataat cataatcggt
     1681 caaagccgca gaataattat cataatcatg caaaatcttt gcgttttcca ttccgataac
     1741 taatccttgt aaacgtaaaa tagtactttg aagaaaatta cgagtgtcat gatcgtcatt
     1801 aaattcattc caatcaatat caggactgct caatgcaggc tgagtatcgt gataatcttt
     1861 tactttgtta ataatcagtt tattttgttc gttaacgcta ccatactttt ggcgttcttc
     1921 tgccgaaaga ttagcttgtt tttctttgag tgcatcctct aaactagaga gtgcattgtt
     1981 tactgccgtt ttttcagcag catcaaaatg acggttgtca aaatttgcaa atggcataaa
     2041 atatttaatt aaaacactgc ctactctagt cagttttcgg cttccctgtt tatcgtaaat
     2101 aatgttgcat atagtgtaca aaaccactcg aagtaatagg ttggggttgg ctttctaggt
     2161 atttatcaac ggcaaaagct atacgaagtc gtggtatttc gggaaattca tcggcagtta
     2221 attgaattaa cagtttgcga tttagaggtt ttgattcttt aaaaagataa ctatcaatca
     2281 attgggagca attatttcta agggtattcc aacacatggc gttcttttta ttgggttaaa
     2341 caataatttt aaaaaattag ttatttggtt ttttacttac taattatcaa tatatagagt
     2401 cgcatacaat taagaaaata gcaaacaaaa ctactatcgt aatgatgttt ttggcttttt
     2461 tgtctgattc taacatgaga gtataatcat tcattaataa ttgtttcata gttttatgct
     2521 tttttagaag ttagtactgc tgtatctttt ttttcaaaaa cctgcccatt tctattaaac
     2581 tttaaaccac caaaattttt tgggcaggtt ttgaaattct aacttaactc aaaaatttac
     2641 ttctaattca ttaaaaaaac attttggcta ttacagaata caaattgttt ctttaactta
     2701 tacaaaggtt ggaattgttg ttgtaaattg cactagggat ttcactagat ttttagatta
     2761 ttttagtgtg agaaacacta ggattttttt cgaaatccta gtgaaatcac tagtccctat
     2821 ttcaaataaa tagtattgct ttgtaacaaa aaacagatta acccaataaa cacaccaatt
     2881 atgtccacaa aaaaactaca actaggcttg gtcgaagaga tggtttctaa ctacaaaaac
     2941 aatcaattat cttctatttt atctagcaca gcgcacccaa tgagctttga tgcccaatcg
     3001 gtatggctcg atttgaatac tctaaaagat tttttaaaaa ctattgaaga agaaactgct
     3061 ttacatcccg aatatgaatt gaaaaacttt ggggtacgtt tctattatgc tgcttatcct
     3121 aaaaaagaca aatgggatca gcaaggatat gaagatttag cctttatgct gacagatcct
     3181 attgcaaaag attatgaaaa attacatact gtaatagcca ttcccaccac agaaatagat
     3241 ggaattaatc aagattttga tccatttgat accaaaacct atgatggtac aaaacctact
     3301 aatactgcgt tggcaattat ggcagagaat catggggtat tatgtccgcc accattttct
     3361 ggttctggtg tttggtttta atatttttaa ttaaaaaact ttttataaag tgattgtatt
     3421 atttaaaatc cttataaaat ctttacttta ttttgagcta cttccagtaa tttttgggat
     3481 tctatatttt aaaaaattta aggatactta ctggaagtac ttcatctatt atttaggttt
     3541 tatttttatt gcaggagctt tacacaattg gataattcaa aaatatttca attatttggg
     3601 agatgatttt ggaaattatt ttttaagtta ttttgtaata cctattgagt ttttattttt
     3661 ctattggctc tatgcctata aatcacttaa ccaaaaaaaa ttgtattgga tatttagtat
     3721 cttgtattta ctttcttttg cctttcatgc tttaataaaa gaaacaatta gttctattta
     3781 ttcttttaac tatgttgttg gtgctttttt tctaggtatt ttagtgtttc tagaatatat
     3841 gaaacaaatt aaatcggatg acattattaa gtttaaagac aacatgatgt tctatgttaa
     3901 tttgggtgta tgtatgttgt atattggcac attacctttc ttttcattct ttggtatatt
     3961 attaaaaaac atggatattt acatgaatta ctatacgatt tttctaatcg taaatcattt
     4021 gatgtactta ctctttacag cagcaattat atggggaaaa ccgaatactt attaatcacc
     4081 attgttttta acttgttttt tttacttttt atggtagcta tcatcacgta tatgttgcaa
     4141 tataaaaaga agaaaaaaga aagtgagatg cttcttgagt ttgaaaaaag caaacaccaa
     4201 agagaacttt tggcttcaca attggaaatg caacaacaaa cgatgcaaga aattggaaga
     4261 gaaattcatg ataatatagg tcagaaatta acgttggcaa gtttatatat tcaaggtaat
     4321 atgtatgaaa acaaaacttt tgaaagcaac gaaaacttgc aaaacattag caacattatt
     4381 aatgaatcat taagtgattt gaggcaactt tctaaaacgt taaccgatga tgctattgcc
     4441 aaaagaaaca tagttgctct tcttcaattg gaatgtgaaa aagtatctag aataaaaaaa
     4501 tgtcttctct atcattatga tgatattgat aaaatagaaa tttcgtacca tcttaaaagt
     4561 atgctgctta gaattgcaca agaatttatt caaaacagtt tgaaacactc cgaatgtgaa
     4621 ggcataaatt tgcatttaag tcatgctgaa aactttattg tcttaaaaat aatggacgac
     4681 ggaaaaggct ttgatgtttc caatccgaat accttaggca ttgggctcca aaatatgaaa
     4741 aagagaacag aaagtgttgg cggtacgttt tttctagaaa gtagtaatac tggaacgcat
     4801 ttaaccctaa aatttccaat tattttataa tataatttat agaatgatta aaattatgat
     4861 tagattagct acgttttaca cctaatcatg aaacactcta ttgttattgt tgacgatcac
     4921 atcctaatag cgaaagcact aggaagcatt atttctaatt ttagagattt tgaagtgctt
     4981 tatgaatgtg gaaatggaaa agaactgcaa gacaaattta aagacaaaaa caacattcca
     5041 aaaatagtat tattggatat tagtatgcca attatggatg gttatgccac ttccaaatgg
     5101 ttgaaagaag cacatccttt ggttttaata ttggcactaa gcatgcaaga tgatgagcaa
     5161 agtttaatca aaatgattaa aaacggatcg aaagggtatc ttctaaaaaa tgtagcacct
     5221 accgaattgg aaactgcttt gcgcagaata gtaaacaaag gacattattt tcccgattgg
     5281 gcatcggata ttgtttttgc aaatatgttt aacaaagaaa ccatcgaaga agttgttgag
     5341 tattttgaaa tatccgatag agaaaaagag tttctatgct tatgtacgac cgaaatgagc
     5401 tataaagaaa tgggcaaaat aatgaagtgt agccctagaa ccattgaata ttatagaaat
     5461 accttgtgta aaaaactaaa ggttaaatca cgcataggac tagctgttta tgctatgaaa
     5521 tcgggattgg taaaagacaa ctattcttcg tagttcttca tttctttatc ataaaactca
     5581 cctgcaagcg taatcaaatg ttccatttcg ctttctagtt cggtttcatc ttcgccttct
     5641 aagtcttcta aaaactcaac ctcatcatct tttaaattga ttaaaaaacg aggaaaatcc
     5701 aaatggatga tgaatatatc gtctggaaaa tcagagttat cggctagtac aaattttggt
     5761 aattgcattt tttagtgatt agtaattagt ctttttgtta gataattaaa gcgaagatac
     5821 aaaaataatg ctgctgcaga caatcctgct agcaatccta tccaaacacc aacagctttt
     5881 aattcggtat ataatcctaa gtaatacgaa attgggaaac ctattagcca ataggcaaca
     5941 aaagtcatgt acatgggtac ttttacatcc tgcaaaccac gcaaagctcc aagcactact
     6001 acttgtatgc catccgaaat ttgaaacaca gcggctacta ataatagatt ggcggctata
     6061 gtaaccactt ccatattggc aaccatattg ataacatcat tgttctttat aaacaacgtt
     6121 gggatgactt catggaaaac cacaaacaac aacgcaaaca ctatttctaa taaaatagcc
     6181 aataaaaata tcgataaagc tactcttctc aatgtaatga aatcggattt tcctttttga
     6241 tttcctactc taatcatggc tgcaacgctt aaacccatag caaacataaa ggtcaacgaa
     6301 gcgatactca acgctacttg atttgccgct tgacttgtta caccaatgat accacaaagc
     6361 catattcctg ccgtaaacaa gacgacttca aacagcattt gcatcgccga tggtgttccg
     6421 agtcggatga ttttgatatt gatttctttc ttaatttctt ttaatgtaaa tcctttaaaa
     6481 tagggatgaa acttcgtctt taagttcatc atataatgca taaaacccaa catgacaaag
     6541 cgagatgcta tcgttccaat agcggcaccc acgatgccca tctttggaaa tatccaaatt
     6601 ccataaatta aaaagtagtt aatgaccaca ttgaccacat tgccaagaat ggttgcccac
     6661 atagagtatt tggtttcgct caatccgtcg gcaaattgtt tataggcttg aaaaacgatt
     6721 agcggaatta atgaaaaacc aacaatgtct agatagggtt ttgccatagt taccacttcg
     6781 tccgattgtc ccatgtaggc aattagtggt ttacagaaat aaattagagc aaacaatact
     6841 atccccaaaa tggtgcataa atacaacccg tgatgaaaca ccgaacgacc ttcttcgatg
     6901 cttttctttc catcagcttc ggctattaat ggtgtgatgg cagtagaaaa accaattcct
     6961 aacgacatag caatgaacac aaaactattt ccaagagaaa cagccgccaa ctcggttggt
     7021 cctaattttc caaccataac gttatctaca atactaacca cagtgtgacc aatcattcct
     7081 aagatgattg gatatgctag ttgcatatta tatttgaatt ctttggtgta attagataaa
     7141 gtcactttgc aattttttcg acggcaaagg taattgattt tttattctaa ctgctttcta
     7201 tattcgtcaa agtttgcttt tacttttggg tctagctgag gctcggctat atcttttagt
     7261 ttgtccagct cttgccaaat ggttttggcc actatgtaac gtgccacgcc tttatcatcg
     7321 gcaggaacga cataccaagg tgcataatcg gttgacgttt tattgatggc ttcttcataa
     7381 tatttcatat aatcgtccca aagcgcgcgt tcttttaaat cgccagtaga gaatttccag
     7441 ttgtcctcag gtgtttctag tcgttttaat aatcgcttgc gttgttcttc tttgctaaga
     7501 tgaagataaa actttagaat cttagtgcca ttttgcgaaa tgtgtttttc aaagttatta
     7561 atttgattaa ttctgttttc ccaaaactgt ggtgttatgt cttctacttt ttcgatgcct
     7621 ggtaggtttt caaacaagat gtattcggga tgcactctag ttactaatac attttcatag
     7681 tgtgttctat taaatacggc aaactttcct ttttcgggta aagctatgta atgacgccac
     7741 aggtaatcgt gttctaactc attagaagtt ggtgttttaa aactttgtac cactacgcct
     7801 cgcgaattaa attctttaaa tacttctcta atcaaactgt ctttaccgct agtatccata
     7861 ccttgcaaac agattaaaaa agcatagcga ttgtgagcat acatcatgtc ttgttttctg
     7921 ctcaatttta cttgaatttc gtccaaagcg gtttcttctt gtttttttga ggtatccatg
     7981 tcaagctctg ttgggatatt atttaatttt attggagaaa tgactttaaa atcatctggg
     8041 tttattgttt tcatcgtatc ctttaatttt tagtattgta aacttatgga aaattttcaa
     8101 ctttcactat tcttaataat ttcagaaata acaccggcat cagaatggtt attattttat
     8161 cactttaaac aaaaagttta acctttctac aactcatccc aaaaaaacaa cacttcggta
     8221 attttaaatt tcatagtttt atgctttgaa gcaactttgc agtatcaaaa atcaaaaaca
     8281 tgaaaatatt ttactttttt ttattctttt gctgtagctt catcaccttt gcacaaaccg
     8341 aaatatcagg aacggttgtt gaccaaaaga acaaaccact tgctggtgcc aacatttata
     8401 tagacggcac ttatgatggt ggtacttcag atgaaaacgg taaattccat tttactacta
     8461 ccgaaacggg ttcaaaaaac ttagtaatta ctatgattac ctacgaatcg tttaacgaaa
     8521 ttattgacgt agccaattgt aaagacaaaa cattcatact aagagaaagt gtaaatactt
     8581 tggatgcagt aattattact gcaggaacat ttgacgctgg cgagaaagca agagtttcgg
     8641 tgctaaaacc tttagacatt gttactactg caggttctaa tgctaatatt atagcggctt
     8701 tgcaaacgtt gccaggaaca caaaccgttg gtgaagatgg tcgccttttt gttcgtggtg
     8761 gcgaagccga tgaaacgcaa acctttgtag atggcattcg agtagcacaa ccatatggag
     8821 ctactactgg aaatgttcca acacgaggac gattttcacc gtttctattt agtggtatgt
     8881 cgttttctac tggtggatat tcggctgaat atggcgaagc tttatcgagt gtactattat
     8941 taaacactac agacgaagcc acagaagaga aaacagatgt ttctttaatg actgttggtt
     9001 taggactagc caatactcag aaatggaaaa acaattctat aacgttcaat acggcttaca
     9061 ttgatttggc accttatcaa aagctaattc ctcaaaacgt ttcttggaac aaaccttttc
     9121 attcgctatc gggtgaaacg gtgtatcgtc attcgtttag caatagtatt ttcaaactct
     9181 atgctgcttt tgatgcttcg gcttttgata ttaaccaaga aagcatcaat agtccgacac
     9241 caacacgtgt agatttgagc aacaacaatt tctatttgaa tgcttcctat aaaactaatt
     9301 tgggtaatca atggaaactg tttaccggaa tggcatatgg ctatggcgaa aacaaaatag
     9361 acatctcaac aggaagagtt tttaatgtag aaaactccaa tcatattaaa gcacgagtaa
     9421 ataaatcatt gaccgataga atcaaaataa cttttggtgg tgattatttc attaccaagt
     9481 ttgatgagta ttttaaatcg acggatgctt ctcgttttga ttatggatat gatgctaaca
     9541 ttgcggctgc ttatgcagaa acggatattt tcttttcccg aaaactcgct ttgaaagtag
     9601 gtgcacgaat ttcgaacaat gattatttaa aagaaacggt gtttacacca agactttcga
     9661 tggggtataa agtttctaaa tcgggacagt tttctttggc gtatgggatg ttttcgcaag
     9721 caccacgaca agagtattta aagtttgccg attatctttc gagcgagaaa gccaatcatt
     9781 atatcttgaa ttatcaatac aacaaagacc gtcaaacttt tagagccgaa atttacttta
     9841 aagagtatgc caatttggta aaatatgatg atgccaatgc caactttgca actgtttaca
     9901 acaacaaagg gcaaggttat gccaaaggaa tcgatatatt ttggagagac aacaagtcta
     9961 ttaagaattt tgaatactgg atttcttatt catttattga taccgagcgc gattataaaa
    10021 actatccaac acaagctacg cctagttttg tagccaatca aagtttatcg gtagtgggta
    10081 aatggtggat tgataaattg aaatcgcaag taagttttac taatagtttc gctaccggaa
    10141 gaccttataa caatccgaat gaaagtactt ttttaggcag caagacgaaa ggatataaca
    10201 atttgagttt gagttgggcg tatttaatta gtcagcaaaa gatattgtat ttctcagctt
    10261 ccaatgtttt gagtacacaa aacgtatttg gctacgaata tgccaacacc ccaaacgcta
    10321 acggaatgta tgaccgtcgt gccatcagac caactgccga ccagttcttt tttgttggtt
    10381 tcttttggac aataagcgaa aataaaaaag acaatcagtt gaataattta taatttcttt
    10441 gcttcttccc aaaaaacatc catctcagcc aaagtcatgt ccgaaagtgg tttgcccagt
    10501 tcggaggctt tggattcgag atacataaac cgtttgataa atttcttgtt ggtgcgctct
    10561 agagcgtctt cggcatttac gtttagaaag cgtgcgtagt taatcatgga gaacatcaca
    10621 tcgccaaact ctgcttctat tttgtcttgg ttgttggttt ttacttcttc ttgaagttcc
    10681 tgcaattctt cttgcacttt gtcccaaact tgatgtggtt cttcccagtc aaagcctact
    10741 ccttttactt tgtcttgaat gcgactggct tttactaatg ctggcaatga ttttggaacg
    10801 ccttctaata ctgatttctt gccttctttt agttttagtt tttcccaatt gcgttttact
    10861 tcttcttcgt ctgccgctac tacatcacca taaatgtgag gatgacgatc gatgagtttg
    10921 tcgcaaatac tattggctac atcggcaata tcaaagttgt tggtttcgct gcctatcttg
    10981 gcataaaaaa caatatgtag cagtaaatcg ccaagttctt ttttaatttc gggcaaattg
    11041 ttgtcaagaa tggcatcgcc aagttcataa gtttcttcga tagtgagatg acgtaatgat
    11101 tcaagtgttt gctttttatc ccaaggacat ttttcgcgca gttcgtccat gatgtctaac
    11161 aatcggttga aggcttttag ttgttcttga cggttgttca ttagtgttga tttgtaattg
    11221 ggataaattt aaaacaatta tgcgacacaa aaaagcatta aaaaaccgtc ctatttcttt
    11281 agtaggacgg ttttcttagg atgtgcattc ataagcatca attatttatc caaaacgatt
    11341 tgattattca ttttacatct ttctttttct caaatgatta ttgaaccttc aacaatcaag
    11401 aaagtcatct actatttgta aaatgttttg acattttcga ttaattaaaa ataaatgtca
    11461 aaaataaatt cctgaattag ctatcgatag gtaccttgta ttgggttgtt tgtatcaaat
    11521 aaacaattgt ctgtaacaaa ggtataactt aaacttgctt gtttcaggtt taatcaagct
    11581 cgaaccaaaa aaagcaactc aaatctgagt tggtatgatt caaaagcatc atagtttgta
    11641 tgaacgtttt tggaaatgaa tcttctaata tagaaaaacc aatactttag aaattttaca
    11701 tccaaattag ttaattatga atttttataa ccaaacgaaa ataaaactaa ctcataaaat
    11761 taacttatat aaaattacat taaaattata tacaactaac aaaaaaaatg ttaatttttt
    11821 atttatataa atctaatata tactactgaa ttcaaaaaaa tgaaaactag gcaagaaagg
    11881 aatgtctcaa aaaaccataa cttgataaga gtaatcatga aggttactct ttcttttcaa
    11941 gcttcttacc tagtaatcca aatctgggga caactttaaa aatacttaac caagcattag
    12001 taacttttaa tcaaaaaaat gaatcttgaa tgtagtgcca aacccaactt tactggtaac
    12061 acttatttta gctttcattg cttctatttg atattttgtg ataaacaatc caatacctgt
    12121 agtttcttgt ttattatgaa atgttttgta taatccgaat aaagcactac cgtgtttttc
    12181 taaatcaatt ccaactccat tgtcggcaat gactagcact ttttggttat tttttgttat
    12241 aagtgagaat tccaatttcg gaaatctatc tggatgcgca tatttgatgg cgttggtggt
    12301 aaagtttatc aatatacttt ctaaatatgc tggagaaaat ttaacaaaat gattttggga
    12361 aatggtgtta acaatctcaa tctttttttt atgtgtataa ctgttgacga tattgt
//
LOCUS       DAOOBH010000066        11993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_6804_length_11993_cov_4.589211, whole genome shotgun sequence.
ACCESSION   DAOOBH010000066 DAOOBH010000000
VERSION     DAOOBH010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11993
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6804_length_11993_cov_4.589211"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="PK218_07670"
     CDS             complement(<1..1907)
                     /locus_tag="PK218_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HPW98422.1"
                     /translation="MKKLIFIRIMLVGYLTTAQNIKSPSNNISLDFLLFSNGKPTYSV
                     NYKNKPVISRSNLGLLLKDGTDFTSGFIVEDTSIITFDEAWQPVLGEQTNIRNNYNQL
                     TVHLIQKATLKKLNIIFKVYNEGVAFRYEFPKQANLNYFIISDEKSEFRLTDDHKAFW
                     IPGDFDSNEYAYNETLLSAIDTEKIDLNNGIGVKSISGKYTVQSPLMMKSTNGIYLNI
                     FEAAVVNYPVMHLELNKENYSFTSKLVPNAIGDKAYLQTPAVSPWRTIMISDDARDIV
                     SSKMILNLNEPSKIDDTSWIKPMKYVGIWWEMHVGKATWDYAGSQNAQNTLDQLKPSG
                     KHGATTENTKRYIDFAAQHGFDGVLVEGWNVGWEDWFGNWKENVFDFTTPYPDFDLKV
                     VNDYAKSKGVKMIMHHETSGSVGNYERHLDRAMNLMKQYGYPAVKTGYVGKIIPRGEF
                     HDGQTMVNHFNFVAQRMAENKLMVNSHESSRPTGYNRTYPNYIAAEAARGNEFNAWSN
                     GNPPMHETILPFTRLLGGPMDYTPGIFEIKMNAYDTTKKEQVHTTLAKQLALYVTMYS
                     PLQMAADLPENYEKHMDAFQFIKDVPADWQESIYLEAEPGDYLTVVRKDKHSEKWILG
                     AITDENARETE"
     gene            complement(1981..3183)
                     /locus_tag="PK218_07675"
     CDS             complement(1981..3183)
                     /locus_tag="PK218_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW98423.1"
                     /translation="MKFGIIKERKNPPDRRVVFSPEELVLLQQKYPEAVIKVESSDIR
                     VFKDEEYTSLGIEVADDISDCEVFFGVKEIPVDYLIPNKKYFFFSHTIKKQPYNRKLL
                     QAILEKNIELYDHETIVDANNKRLIGFGRYAGLVGAYNGFRGFGIKYDLFNLPKAETL
                     HSKEDLIARIKRHTLPNIKIVVTGHGKVGMGIKEILDGVKIKQVSPQDFLTKNYSQPV
                     YTQIDVLDYNKRKDGVKLDKQDFYKNPQDYISDFERFTKVADIYMSGHFHGNNAPDIL
                     TREMLNASNNKIQVVADISCDVDGPIACTLKASTIAEPFFGYLPSEHKEVSHTHPASI
                     MVMSVDNLPCELPKDASEGFGVMFMEHVIPAFFNGDKDGILKRAQMTENGKLTSRFAY
                     LQDYVDGK"
     gene            3343..4545
                     /locus_tag="PK218_07680"
     CDS             3343..4545
                     /locus_tag="PK218_07680"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPW98424.1"
                     /translation="MKFNSLYKSLTLLFVLTILFSCSKQEKTEEGVVKINENGLPIMQ
                     PLQEPLPKLSDQFVKDKKFGVSMFFEKTWSEKNDNVSFLVAKNGQIIYENYMGFANKR
                     TDEKITADTPLHIASVSKVLTATATLMLVDAKKLTLDQKVNTIIKEFPYPEVTVQTLL
                     NHRSGMKNYAYFTYENNNWDKKKILTNEDIVKVMVEKEIPLETKTDTHFSYCNTNYAV
                     LALIIEKITGLTYPEAMKEMIFAPLGMKNTFVCEFEKDRENIVPSYKGNNTEIGTDYL
                     DAIYGDKNIYSTPRDLLLFDTARYAPFFLNADLIKKIYQGYSYESKGIRNYGLGIRIS
                     EFEKGEPFYYHNGWWHGNTSSFLNLRKEKVTIIVLSNKFTKKTYQTKKLASLFGDYPF
                     KLNDDGEE"
     gene            complement(4566..5075)
                     /locus_tag="PK218_07685"
     CDS             complement(4566..5075)
                     /locus_tag="PK218_07685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW98425.1"
                     /translation="MKIIKATKEDLSAMMHIIHQAQEYLASLNIDQWQNGYPNEQKIL
                     EDIANQESYLIKNEQNQIIAITMFTTRNELTYAVIEGNWLTNDNTTYGVIHRMAVNNE
                     YRNAGAAKFIFHYFEQLLKAENIVSMRIDTHQDNIGMQHILTKLDYKYCGIIYLEDGN
                     KRLAYEKII"
     gene            complement(5125..7152)
                     /locus_tag="PK218_07690"
     CDS             complement(5125..7152)
                     /locus_tag="PK218_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98426.1"
                     /translation="MTNTTILLLLLSVVIAGILSFYQYYYKAKSKSKVNLLLAFLRFI
                     SIFGILLLLINPIISRKTIEVIKTPLPIIVDNSASVKDLKADQEALGVFKALSENKAL
                     QEKFEVQTYQFDSDVSPADSITFSGKQSRIDLVAKNLKSIYKNQLFPTVLISDGNQTS
                     GEDFVFSFNPENKVYPVAVGDTTTYMDLRISQLNVNKYAFHKNKFPVEVFLNYSGTKS
                     INATFKIGQGNTVLNEQTVSFSPSKKSASFSVLLPADRVGVQVFRASITSKEQEKNTY
                     NNSKNFAIEVMDQRTEVALISAIPHPDLGAIKRSIETNSQRKVTIVKPNEIKSLLDYN
                     VLVLYQPNVSFKTVFDQNKNAKINTWIITGNNTDYTFLNQNQEDYTFKMSGQSENYLS
                     TFEKQFNLFAIDDFGFEQFPPLENQFGTITSKSNQNVLLSSRIRNIETNQPLLSFSDN
                     QGRRNAYLFGENIWKWRADVYINNKSFEKFDVFADKIIQFLASNDSKKSLVVSHERFY
                     NSGEAIEINAQYFNKNYEFDEKARLTITVTNSTNKQQKKYDLVRFSNSFKVNLDGLAA
                     GKYSFTVKELNSNSSYNGNFEVLDFDIEKQFVNPDWNKLNQLAVQTNGKAYLANQSET
                     LIQKLLADESYKAMQKSIVKKSPLIDWIWLLVLIVTMLGAEWFIRKYNGLL"
     gene            complement(7205..7576)
                     /locus_tag="PK218_07695"
     CDS             complement(7205..7576)
                     /locus_tag="PK218_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPW98427.1"
                     /translation="MRIFSFEKLIAWQKSRELALMTFKCTKKYPKDELFGLTSQMRRC
                     SISIASNLAEGSGRSSYKDKARFTEIAYSSSLELLNQVILSVDFEYIDENEYITIREK
                     ITEVNMLIDGLHKSQKSNFKV"
     gene            complement(7629..8375)
                     /gene="fabG"
                     /locus_tag="PK218_07700"
     CDS             complement(7629..8375)
                     /gene="fabG"
                     /locus_tag="PK218_07700"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPW98428.1"
                     /translation="MKLLEGKVAIITGASRGIGSGIAKVFAENGANVAFTYSSSVESA
                     TALENELSGLGIKAKGYKSNAADFNEAQKLIDDVMAEFGTIDVLINNAGITKDNLLMR
                     MSEEDFDKVIEINLKSVFNMTKAVQKVMLKNRKGSIVNMSSVVGVKGNAGQANYAASK
                     AGMIGFTKSIALELGSRNIRCNAIAPGFIETEMTAKLNEDVVQGWRDSIPLKRGGTPE
                     DVANACLFFASDMSAYVSGQVLNVCGGMLT"
     gene            8506..9057
                     /locus_tag="PK218_07705"
     CDS             8506..9057
                     /locus_tag="PK218_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPW98429.1"
                     /translation="MQNNAFDLNFLYQTYNLLVYNVALNYVQNVEDAEEITQDVFVKV
                     NYSLKNFNQKSSLKTWIYRITVNQSLDFIKKKNSQKRFFIFGKRNENEYDYLNASAFE
                     HPGIELEKKEEAELLFTTINTLPENQKTAFILSKIDGLSNPVVAEIMEISISAVESLV
                     FRAKSSLKDKLAHKYLEYRKNKK"
     gene            9099..9371
                     /locus_tag="PK218_07710"
     CDS             9099..9371
                     /locus_tag="PK218_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98430.1"
                     /translation="MEKENWIETVMESTNGITKVTPSKTLFSKITFKINQEKKASPQT
                     IWLVAASILVLVLLNIAAIKTQRFSKQETSSVYLSGTLNQSNQLYQ"
     gene            9373..9867
                     /locus_tag="PK218_07715"
     CDS             9373..9867
                     /locus_tag="PK218_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98431.1"
                     /translation="MEKSKLNSIAIIVLIVLNLCTLGFIFFNSSKNHRPPMDRMNPKD
                     IIIKKLRLDESQQKDYTKLIEWHQNEIQKHDKEIVNAKKSLYGLLINNEIDLKSKDSL
                     ITVINLNQKQIEETHFKHFEDIKKLCKPEQLDDFKEFSKELGRLFAPNKQRKEKRESE
                     RPKP"
     gene            9869..10570
                     /locus_tag="PK218_07720"
     CDS             9869..10570
                     /locus_tag="PK218_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98432.1"
                     /translation="MFKNFGTPLLIVLLFCLHSTAQTTTIPIRFKLKFNSNDVVKGQN
                     FISKNNDTLSIETFKCYLSNFKLEYTDKSISEIKNHYYLLDSDSLKSFRIELPKKENK
                     TISKITFAIGIDSITNTSGVQNGALDPINGMYWAWQSGYINFKIEGKSSSCKTRLNKF
                     HFHIGGYLYPNYALRIIEIPIHKSQNPTKTLDLTIDLATFFNEIHLEENNSIMIPGKK
                     AMELADLSVKLFSEK"
     gene            10567..11589
                     /locus_tag="PK218_07725"
     CDS             10567..11589
                     /locus_tag="PK218_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HPW98433.1"
                     /translation="MKKLTFISVFGILFFSVLGFTNLNFTPIYFEVPKHFPKPVYDFS
                     KNPLTEEKFQLGRTLFYDPILSRDNTISCASCHLQASAFTHVDHDLSHGIDGRIGTRN
                     SLTLQNLAWSKDFMWDGGINHLDVQAITPITHQLEMDETLPNVIKKLQESPKYNALFT
                     QAFGDKKVTSERLLKSFSQFLVQLVTANSKYDKVIRKEETYTSQEQNGYTLFQNNCAS
                     CHTEPLFTSDVFETNGLPVDAYLKDGGRIKITTLKKDSLLFKVPTLRNIQFSFPYMHD
                     GRFKTLNEVVLFYNTNPDAQKALLKKNKKRMQLTDNERVDLITFLKTLSDKEFLFNPR
                     YSFPRE"
     gene            complement(11621..>11993)
                     /locus_tag="PK218_07730"
     CDS             complement(11621..>11993)
                     /locus_tag="PK218_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98434.1"
                     /translation="KITKKTISISNLPYNFLTVFKHFITFFRFKIKVAHHFGNGADEK
                     VDGIHPVGNVADEKVNGIHLVGNGADEIVDVADEKVDGIHPVGNGADEKVDGIHLVEN
                     GVQMVGDFFNVFNFFGTSLNY"
BASE COUNT         4010 a         2032 c         1828 g         4123 t
ORIGIN      
        1 atttcagttt ctcttgcatt ttcatcagta atagcaccca aaatccattt ttcggaatgt
       61 ttgtcttttc gaacaaccgt taaataatcg cctggttcag cttccaaata aatactttct
      121 tgccaatcgg caggaacatc tttaataaac tgaaatgcat ccatatgttt ctcgtagttt
      181 tcaggtaaat cggcagccat ttgcagcggt gaatacatag tgacgtaaag tgctaattgt
      241 ttagctaaag tagtatgaac ctgctctttt tttgtagtat cataagcatt cattttaatt
      301 tcaaaaatgc ctggtgtata atccattggc ccaccaagaa gccttgtgaa aggcaaaata
      361 gtttcgtgca ttggtggatt tccgttactc caagcattaa attcgtttcc tctagcggct
      421 tcggcagcaa tatagtttgg ataggttcta ttgtagcctg ttggtcgtga gctttcatga
      481 gagttgacca tcagtttatt ttctgccatg cgttgtgcta caaagttgaa atgattaacc
      541 attgtttgtc catcatgaaa ttcgccacgc ggaatgattt tcccaacata acccgttttg
      601 actgccggat atccgtattg tttcatcaaa ttcatagctc ggtctagatg acgttcataa
      661 ttacctactg aacctgatgt ttcgtgatgc attatcattt ttacaccttt cgacttggca
      721 tagtcattaa caacttttaa atcgaaatcg ggataaggtg tagtgaaatc gaacacattt
      781 tctttccaat ttccaaacca atcttcccaa ccaacgttcc aaccttctac taaaacacca
      841 tcaaacccgt gttgtgctgc aaaatcgata tagcgtttag tgttttcggt ggttgcgcca
      901 tgtttacctg atggtttcaa ttggtctaat gtgttttgtg cattctgact gccagcatag
      961 tcccaagttg ctttaccaac atgcatttcc caccaaatac caacatattt cataggtttt
     1021 atccacgaag tatcgtctat tttggatggt tcattaagat ttaaaatcat tttagaacta
     1081 acaatatctc tggcatcatc actaatcata atggttcgcc aaggagaaac agctggtgtt
     1141 tgaaggtaag ctttgtcacc gatagcattt ggcactaact tagaagtaaa cgaataattt
     1201 tctttattca actctaaatg catcactgga tagtttacca cagcagcttc aaagatattt
     1261 agataaattc cattagttga tttcatcatc aatggcgatt gaacggtata ttttccagaa
     1321 atagacttta ctcctattcc attattcaga tcaatttttt cggtatcaat tgctgataat
     1381 aacgtttcgt tataggcata ttcattgcta tcaaaatcac caggaatcca aaaggcttta
     1441 tggtcgtcgg tcaatctgaa ttctgatttt tcatcactaa tgataaagta atttaagtta
     1501 gcctgttttg gaaattcata ccgaaaagca acaccttcat tatatacttt gaatattata
     1561 ttgagctttt ttaaagtcgc tttctgaatt aaatgaacgg ttaattgatt gtaattgttt
     1621 ctaatgttag tttgctcacc aagaacaggt tgccatgctt catcaaaagt aattatactc
     1681 gtatcttcta ctatgaatcc tgaagtgaaa tcggtaccat cttttaagag caaacccaag
     1741 ttgcttctag aaataactgg tttattttta tagtttacgg aataagttgg ttttccattt
     1801 gagaataata aaaagtcaag acttatattg tttgatggag atttgatatt ttgtgcagta
     1861 gttaaataac caacaagcat tatacgaata aatattaatt ttttcatttt accttttttt
     1921 aataaaactt ttataaaaat agaaaatcct gatggagtat caggattttt ttatcattag
     1981 ttacttacca tcaacataat cttgcaaata tgcaaacctt gatgtcaatt ttccgttttc
     2041 ggtcatttgt gctcttttca atataccgtc tttatcacca ttaaaaaaag cgggaattac
     2101 gtgttccata aacattactc caaaaccttc actagcatct tttggcaatt cgcaaggtag
     2161 attatcaact gacattacca taatagatgc tggatgcgtg tgtgaaactt ctttgtgttc
     2221 actaggcaaa taaccaaaga atggctctgc aattgtagaa gcctttaaag tacaagcaat
     2281 tgggccatca acatcgcatg aaatatcggc gactacttga attttgttat ttgaagcatt
     2341 taacatttct ctagttagta tatcgggtgc attgttacca tgaaaatgtc cagacatata
     2401 aatatcagct actttggtaa aacgttcaaa gtcggaaatg taatcttgtg gatttttata
     2461 gaaatcttgt ttatctagct ttacaccatc ttttctttta ttataatcca acacatcaat
     2521 ttgtgtataa acaggttgcg aatagttttt ggttaaaaaa tcttggggag aaacttgttt
     2581 tattttaaca ccgtcaagaa tttctttaat tcccatacct acttttccgt gacctgtaac
     2641 cactattttt atgttgggca acgtatgtct ttttattctg gcaattaaat cttctttgct
     2701 atgtagtgtt tcggcttttg gcaaattgaa taaatcatat ttgattccaa atcctctaaa
     2761 accattgtaa gcaccaacca agcctgcgta tcttccaaaa ccaattaatc ttttattatt
     2821 tgcatcaaca atcgtttcat gatcatacag ttcgatgttt ttttcgagaa tcgcttgcaa
     2881 aagcttgcgg ttatagggtt gctttttgat agtatgcgaa aagaaaaaat attttttatt
     2941 tggtatcaaa taatccactg gaatttcttt aacaccaaag aaaacttcac agtctgaaat
     3001 atcatctgct acttcaatcc ctaaagaggt atattcttca tctttaaaca ctctaatgtc
     3061 tgagctttct actttaatga cagcttcagg atatttttgt tgtaatagta ctaattcttc
     3121 aggagaaaaa acaactcttc tgtctggtgg atttttgcgc tctttgatta ttccaaattt
     3181 cattttcata tttttttgct tgccaaaagt aagggtaacg aaaacgtttt caaaactttt
     3241 ttacttaaaa tccattgtgg aaaacctgtt gatagcttta ttggaaacct tttttaaaaa
     3301 atgtttgaat cgttatattt gtttttatac aagtctcaaa aaatgaaatt caattctcta
     3361 tataaatcat taacattact atttgttcta actattttat tctcttgttc taagcaggag
     3421 aaaacagaag aaggtgttgt taaaataaat gaaaatggtt tgccaattat gcaaccgctt
     3481 caagaacctt tacctaaact atccgatcaa tttgtgaagg ataaaaagtt tggtgtatct
     3541 atgttttttg aaaaaacgtg gtctgaaaag aatgataatg ttagtttttt ggttgccaaa
     3601 aatggtcaaa tcatttatga aaactacatg ggttttgcca acaagcgtac cgatgaaaaa
     3661 atcaccgcag atacgccatt acacattgct tctgtaagca aagtattgac ggcaacagca
     3721 actttaatgt tagttgatgc aaaaaaatta actctagacc aaaaagtaaa caccattatt
     3781 aaagaatttc cttatccaga ggttacggta caaacattac taaatcacag aagtggcatg
     3841 aagaattatg cttatttcac ttatgaaaat aataattggg acaagaaaaa gatactgacc
     3901 aacgaagaca tcgtaaaggt tatggtagaa aaagaaattc cattggaaac aaaaactgat
     3961 acacatttta gttattgcaa tactaattat gctgttttag cattaattat agagaaaatc
     4021 actggtctaa cttatcctga agcgatgaaa gaaatgattt ttgcgccact tggtatgaaa
     4081 aatacttttg tgtgtgaatt tgaaaaagac agagaaaaca ttgtgccgtc ctataaagga
     4141 aacaatacag aaattggtac cgattatctg gacgctattt atggcgataa aaatatctat
     4201 tctacaccac gtgatttatt actttttgat actgctcgtt atgctccatt tttcttaaat
     4261 gctgatttga ttaagaaaat atatcaaggt tatagttatg aaagtaaagg tatccgcaat
     4321 tatggacttg gaataagaat aagtgagttt gaaaaagggg aaccttttta ttaccacaac
     4381 ggttggtggc atggcaatac gtcttcgttt ctaaatttga gaaaggaaaa agtaacgata
     4441 attgttttat ctaataaatt taccaagaaa acctatcaaa ccaaaaaact tgcatcatta
     4501 tttggtgatt atccgttcaa gttgaatgat gatggcgaag aataattctt tttgaaagct
     4561 agttactata ttattttttc gtaggccaat cgcttgttgc catcttcaag ataaataata
     4621 ccacaatact tgtaatctaa cttagtgagt atgtgctgca ttccaatatt atcttgatgc
     4681 gtatcaattc gcatagaaac aatattttcc gccttaagca attgctcaaa ataatggaat
     4741 ataaactttg ctgcgccagc atttcgatac tcattattta ctgccattct atgaataacg
     4801 ccataagttg tgttatcgtt tgttaaccaa ttaccttcta tgacggcata agttaattca
     4861 tttcgagtag taaacattgt tatggcaata atttgatttt gttcgttttt tattaaataa
     4921 ctttcctgat tcgcaatatc ttctagtatt ttctgctcat taggataacc attttgccat
     4981 tggtcaatat ttaatgaagc aagatattct tgtgcttgat gaattatatg catcattgca
     5041 gaaagatctt ctttagtggc ttttataatt ttcatttagc ctttattgtg tatgaatgag
     5101 tcaaaataaa ttatccttat aaagctacaa caatccattg tattttctaa taaaccattc
     5161 tgcaccaagc attgttacta tcaaaaccaa taaccaaatc caatcaatta atggcgattt
     5221 tttaacaatg cttttttgca tggctttata actttcatca gccaataact tttgaatcaa
     5281 tgtttcggat tggtttgcca aataagcttt tccattagtt tgtaccgcta gttgattcaa
     5341 tttattccaa tctggattta caaattgctt ttcgatatcg aagtctaata cttcaaaatt
     5401 gccgttatag cttgaattgg agttcagttc tttaacagta aatgaatatt ttccagcagc
     5461 caatccgtca agatttactt taaatgaatt ggaaaaacgt actaaatcgt attttttctg
     5521 ttgtttgtta gtcgaattgg ttaccgttat cgtcaatctt gccttttcgt caaattcata
     5581 gtttttattg aaatattgcg cattgatttc aatagcttcg ccagaattgt aaaagcgctc
     5641 atgcgaaact actaatgatt ttttggaatc attagaagcc aagaattgaa ttattttatc
     5701 cgcaaaaaca tcaaatttct caaaagactt gttgttgata taaacatctg cgcgccattt
     5761 ccaaatattt tctccaaata aatatgcatt tcttctacct tgattgtccg aaaaagacaa
     5821 taagggttgg ttggtttcaa tgtttctaat tctcgaagat agcaacacgt tttgattgct
     5881 ttttgaagta atggttccaa attgattttc taatggtgga aattgttcaa aaccaaaatc
     5941 atcaatggcg aacaagttga attgcttttc aaaagttgac aaatagtttt ccgattgtcc
     6001 actcatttta aatgtataat cttcttgatt ttgatttaaa aaagtataat cagtattgtt
     6061 tcctgtgata atccatgtat taattttagc atttttgttt tgatcaaaaa cagttttaaa
     6121 acttacgttt ggctgatata aaaccaaaac attgtaatca agtaatgatt tgatttcatt
     6181 aggcttaact atcgtcactt tacgttgcga attagtctca atacttcttt ttatagcacc
     6241 taagtcagga tgtggaatag cacttattaa tgctacttct gttcgctgat ccattacttc
     6301 aatggcgaaa ttcttggaat tgttataggt gtttttttct tgttctttgg atgtaatgct
     6361 tgctctaaaa acttgaacac ctactctatc tgctggaagt aaaacagaaa acgatgccga
     6421 cttttttgat ggtgaaaaag aaaccgtttg ctcgtttaga acagtatttc cttggccaat
     6481 tttaaatgtt gcattgatac tttttgtacc gctataattt aaaaatactt ctactggaaa
     6541 tttgtttttg tgaaaagcat acttattcac atttaactgc gaaatacgca aatccatata
     6601 agtcgttgta tcgcccacag caactggata tactttattt tctggattga aactaaatac
     6661 aaaatcttcc cctgaggttt gatttccatc agaaattaaa actgttggaa ataattggtt
     6721 tttataaata ctttttaagt ttttggcaac caaatctatt cgagattgct taccactaaa
     6781 cgtaatagaa tcggcaggag aaacatcaga atcaaattga tacgtttgta cttcaaactt
     6841 ctcttgaagt gctttgtttt cggataatgc tttgaaaaca cctaaggctt cttggtctgc
     6901 ttttaaatct tttacagaag ccgaattgtc aactataatt ggcaaaggcg ttttgataac
     6961 ttctattgtt ttccttgaaa taattggatt tattaataat aacaaaatcc cgaaaatgga
     7021 aataaaacgc aaaaaagcta aaagcaaatt aactttagac ttgcttttag ctttataata
     7081 atattgataa aatgacagta ttccagcaat tacaactgaa agtaaaagta gtagaatagt
     7141 tgtatttgtc attataatgg ttaaaagttt aggagtttag gagtttaaaa gtataaaagg
     7201 tgacttacac cttaaaattt gatttttgag atttgtgtaa accatcaatt aacatgttaa
     7261 cttctgtaat tttctctcta attgtaatgt attcattttc atcaatatat tcaaaatcga
     7321 cacttaagat aacttgatta agcaattcca atgacgagct atacgctatt tcagtaaatc
     7381 gagctttgtc tttataagaa cttcgacccg aaccttccgc caaatttgaa gcaattgata
     7441 ttgaacaacg acgcatctgg ctagttaatc caaataattc atctttagga tattttttgg
     7501 tgcatttaaa agtcattaaa gctaattctc gagatttctg ccaagcaatc aatttttcaa
     7561 aactaaatat tctcataact taattttatt acttttaacc ttttaaacgc ttaaactttt
     7621 aaactctcct aagtcaacat tcctccacaa acatttaata cttgtcccga aacataggca
     7681 ctcatatcag aagcaaagaa caaacaagca ttggctacat cttctggtgt tccgccacgt
     7741 ttcaatggaa ttgaatccct ccaaccttgc accacatctt cgtttaattt tgccgtcatt
     7801 tcagtttcta taaatcctgg tgcaatagca ttgcaacgaa tgtttcttga acccaattct
     7861 aaagcaattg atttagtaaa accaatcatt cctgcttttg atgcagcata gttagcttgt
     7921 cctgcatttc cttttacacc aacaaccgaa ctcatgttga caattgaacc tttacggttt
     7981 ttaagcatta ctttttgcac cgcttttgtc atgttgaaaa ctgattttaa gttgatttcg
     8041 attactttat caaaatcttc ttcgctcatt cgcattaaca aattgtcttt tgtaattccg
     8101 gcattattga taaggacatc gatagttcca aattcagcca taacgtcgtc aattaatttt
     8161 tgagcttcgt taaaatcggc agcatttgat ttatatcctt ttgcttttat tcctaaacct
     8221 gataattcat tttctaaagc tgtagctgat tctactgatg agctataagt gaaggcaaca
     8281 tttgctccgt tttcggcaaa cacttttgca attccacttc caattccacg acttgcgcca
     8341 gtaatgatgg ctacttttcc ttctaataat ttcataaatg tgattttaaa aaatacaaag
     8401 gtcaaatata ctattatttt gaggttttaa aaaaatagat tcttaagata ctcacacaag
     8461 aaatgattat ttttagcgtc aaagtaatta tagaatctca tcttaatgca aaacaacgct
     8521 tttgatttaa actttttata tcaaacgtat aacctattgg tctataacgt tgcgttaaat
     8581 tatgttcaaa atgtagagga tgcagaagaa attactcaag atgtatttgt taaagtaaat
     8641 tattcgctga aaaattttaa tcaaaaatct tctcttaaaa cttggatata tcgcataaca
     8701 gtaaaccaat cgttggattt tattaagaag aaaaacagtc aaaaacgctt ctttattttt
     8761 ggaaaaagaa acgaaaacga atatgattat ttgaatgcaa gcgcttttga acatcctgga
     8821 attgaattgg aaaaaaaaga agaagcggaa ctattgttta caaccatcaa tactttgccc
     8881 gaaaatcaaa aaacagcttt tatactttca aaaattgatg gtttatcaaa tcctgtagta
     8941 gcagaaatta tggaaatctc tatttcggca gtagaatcat tagtgtttag agccaaaagc
     9001 agtttaaaag ataaattggc tcataaatat ttagaatacc gcaagaataa aaaataaaat
     9061 acgtctaaaa aagtgtgaag aatttaaaat tcatgattat ggaaaaagaa aattggatag
     9121 aaacagtaat ggaaagtact aatggaataa cgaaagttac tccaagtaaa actttgttct
     9181 caaaaattac ttttaaaatc aatcaagaga aaaaagctag tccacaaact atttggttag
     9241 ttgcagcatc tatactcgtt ttagtattat taaacatagc tgcaattaaa actcaacgtt
     9301 tttcaaaaca agaaacaagc tcagtttact tatcaggaac actaaatcaa agcaatcaat
     9361 tatatcaata aaatggaaaa atcaaaatta aatagcattg ccataattgt gttaattgtg
     9421 ttaaatctct gcactttagg atttattttt ttcaattctt ctaaaaatca tcgtccgcca
     9481 atggacagaa tgaatcctaa agatattatt atcaaaaaat tgcgtttaga tgaaagtcaa
     9541 caaaaggatt atacaaaatt aatcgaatgg catcaaaatg aaattcaaaa acatgataaa
     9601 gaaatagtga atgccaaaaa aagcctttat ggattgctga tcaataacga aattgatttg
     9661 aagagtaaag atagtttgat tactgtaata aatttgaatc agaaacaaat tgaagaaaca
     9721 catttcaagc attttgaaga catcaaaaag ctttgcaaac cagaacaatt agatgatttt
     9781 aaagaattct ctaaagaatt aggaagactt tttgcaccaa acaaacaacg aaaagaaaag
     9841 agagaatcag aaagaccaaa accttaatat gtttaaaaat tttggaacac cgttattaat
     9901 tgtcttacta ttttgtttac atagtaccgc acaaactacg actattccaa ttcgttttaa
     9961 gttgaagttt aattcaaatg atgtcgttaa aggtcaaaac tttattagca aaaacaacga
    10021 tactttaagc attgaaacct tcaaatgtta tctttctaat ttcaaattag aatataccga
    10081 taaatctatt tctgaaatta aaaaccatta ttatttatta gatagtgata gtttgaaatc
    10141 ctttcggatt gaacttccaa aaaaagaaaa caaaacaatt tctaaaatta cttttgccat
    10201 cggaattgat agcatcacca acacttctgg agttcaaaat ggagcattag acccaattaa
    10261 tggaatgtat tgggcatggc aaagcggcta tatcaatttt aagattgaag gtaaaagttc
    10321 gagttgcaaa actcgactga ataaatttca ttttcatatt ggtggatatc tttatcccaa
    10381 ctatgcctta agaattattg aaattccaat acataaatcc caaaacccaa ctaaaactct
    10441 tgatttaacc atcgacttag caacattttt caatgaaatt catttagagg aaaataactc
    10501 tataatgatt cctggaaaaa aagctatgga actagccgat ttatctgtaa aactattttc
    10561 tgagaaatga aaaaattaac atttatatcg gtatttggaa ttttgttttt ttctgttttg
    10621 ggatttacca atttgaactt cactcctatt tactttgaag ttccaaaaca ttttccaaaa
    10681 ccagtttatg atttttccaa aaacccatta accgaagaaa aatttcagtt gggcagaaca
    10741 ttattttacg acccaatttt atctcgagat aacactattt cgtgtgccag ttgccatttg
    10801 caagcttctg ctttcaccca tgttgaccac gatttaagtc atggaattga tggtcgaatt
    10861 ggtacacgca attcattgac attgcaaaac ttagcttgga gcaaagattt tatgtgggat
    10921 ggtggcatca atcatttaga tgtgcaagcc attactccta ttacacatca attggaaatg
    10981 gatgaaacac ttccaaacgt gattaaaaaa ctgcaagaaa gtcccaaata caatgcgtta
    11041 tttactcaag cctttggaga taaaaaagta acttcggagc gacttttaaa gtcattttca
    11101 caatttttgg tgcaattagt aaccgcaaat tccaaatacg ataaagtaat tcgcaaagaa
    11161 gaaacctata catcacaaga acaaaatggt tatacgctat ttcaaaacaa ttgtgcttcg
    11221 tgtcataccg aacctttatt tacaagtgat gtttttgaaa ccaatggatt gcccgttgat
    11281 gcgtatttaa aagatggcgg aagaataaaa ataaccactt taaagaaaga cagtttgctt
    11341 tttaaagtgc caacattgag gaacattcaa ttttcgtttc cctatatgca cgatggtcgt
    11401 tttaaaactc taaacgaagt ggtacttttc tacaacacca atccagatgc acaaaaagca
    11461 ttgctcaaaa agaacaaaaa aagaatgcaa ttaaccgaca acgagcgtgt tgatttgatt
    11521 acttttttaa aaaccttatc ggacaaagag tttttattca atccgcgtta ctctttccct
    11581 agagaataat ttcttgattt taaagcctaa aagtcatctc ctaatagttt aaagatgtcc
    11641 caaaaaaatt gaagacgtta aaaaagtcgc ccaccatttg gacaccgttc tccaccaaat
    11701 ggattccgtc cactttttcg tcagcaccgt tccccacggg atggattccg tccacttttt
    11761 cgtctgcaac gtccactatt tcgtcagcac cgttccccac gagatggatt ccgttcactt
    11821 tttcgtcagc aacgttcccc acgggatgga ttccgtccac tttttcgtcg gcgccgttgc
    11881 cgaaatggtg ggcgactttt attttaaatc taaaaaaggt gataaaatgt ttaaaaacag
    11941 ttaaaaaatt gtaaggcaaa tttgaaattg aaatggtttt tttggttatt ttt
//
LOCUS       DAOOBH010000067        11956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_6834_length_11956_cov_4.724309, whole genome shotgun sequence.
ACCESSION   DAOOBH010000067 DAOOBH010000000
VERSION     DAOOBH010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11956
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6834_length_11956_cov_4.724309"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..2287
                     /locus_tag="PK218_07735"
     CDS             320..2287
                     /locus_tag="PK218_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPW98435.1"
                     /translation="MFKKLLQITCFFAVLSTYSQVVINEIDADTPGTDTKEFIELKSV
                     TANFSLDGYVLVFLNGGSTGTSNASYLALDLDGFTTDINGLFLIGNNQVSPTPTYIIP
                     NASIQNGPDVVALYQANASDFPLNTIGTSSNLIDAVAYSSSTSSSPTAIMSALGITTF
                     GIDNTNSSANTSIQRNNDGTYTSATPTPGQNNDGSGVVLNHVTITASATTVNEGDTIV
                     FTFTTETPVVGSNLIINMVVNNGTFNLQDINGTLTSFIPVGQTSISNSLLIVNDGINE
                     GDEELLLNVQSLQPGFVLNNNYLLIRINESNFEVKPWGTPANPTYGVVAPTYPAGYYS
                     SLEGLSGAALKQALQNIIANPQVVRAHTYADIWEILKTADQNPENSSQVWLIYNETPR
                     SKLDQQATSSIVGKWNREHIYCQSRGNFGDWYDTSATGINNYVLSNAGDIAAGLSDAH
                     HLRAADGQENSSRGNRNYGVDYNGPTGNPGSWKGDVARALFYMAVRYNGLNVVNGNPV
                     DNTIGQIGDLTTLLSWNQQDTSDDFEMNRNNYIYTWQMNRNPFIDYPSLVNYVFGANY
                     GQPWFSSLATTEINFDKIVLYPNPVNDYLSIIGLNTKGTLELFNTLGEKVFQSDIENQ
                     HQINLNLQSGVYFAKISSENKNFTKKIIITN"
     gene            2295..2828
                     /locus_tag="PK218_07740"
     CDS             2295..2828
                     /locus_tag="PK218_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98436.1"
                     /translation="MNAGFNKKESSIALKAELIFKLTQGLIEIVPKDNKLFLQETTVR
                     FMLEDAMMIPAKISGAEAIDLYDIKMENATIIRKCAQQLYVNAGNLRFEESIKDSEYI
                     ELLRDEIEEFRLLFIDWVASFDQWNYIIDRWGLFNPPGVTAHDKDPDDSISFNSKEYF
                     DGLDLDNLDEDENDDDK"
     gene            complement(2847..3854)
                     /locus_tag="PK218_07745"
     CDS             complement(2847..3854)
                     /locus_tag="PK218_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPW98437.1"
                     /translation="MAINKNALIRYHALDKCFSNFGRRFSNEDLLEVCNEALAEYNYQ
                     SEGIKKRQLFEDIKFMESSQGWEIPLERTKEGRTVYYRYADKDFSINNQPINDFEAEK
                     LKSALLVLARFKGMPQFEWVNELILKLQQAFNLQSHTKEIISFDANIYLKNIHLIGDL
                     FNAILYEKVVQIEYQKFNNAESKIHEIHPYYLKQYNNRWFLIGRFEKYENLTHFAIDR
                     MISVQETNAKYISTTVDFNEYFEDVIGILKPKDALIEKIILLATPELKPYLTTKPIHG
                     SQKKISDDENGYLFSIELIINYELENLLLSYGETLKIIAPIHLQETIKSRLQKNLENY
                     I"
     gene            3960..4286
                     /locus_tag="PK218_07750"
     CDS             3960..4286
                     /locus_tag="PK218_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HPW98438.1"
                     /translation="MREHLAHFDIAGFTYYEGVLAFSELEIGTLLQLKLEEDNKFDAR
                     AVALYFKNFKLGFVPRSENRIFYKFLKVGISDCFEVRIQRIDKKEHPENQIGVIVHLV
                     RKVAQE"
     gene            complement(4668..6086)
                     /gene="mnmE"
                     /locus_tag="PK218_07755"
     CDS             complement(4668..6086)
                     /gene="mnmE"
                     /locus_tag="PK218_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPW98439.1"
                     /translation="MINQETIVALATPSGAGAIAIIRLSGNEAISVASKVFQSVSGKD
                     LSKQKSHTIHLGHIVENTKIYDQVLVSVFKNPNSYTGEDVVEISCHGSTFIQQQIIQL
                     LLRKGCKMAQAGEFTLRAFLNGKLDLSQAEAVADLISSDNEASHQIAMQQMRGGFSNE
                     IAQLRQELLNFASLIELELDFAEEDVEFADRTQFKELVTRIEFVLKRLIDSFAIGNVL
                     KNGIPVAIVGEPNVGKSTLLNALLNEERAIVSDIAGTTRDTIEDELVIDGIGFRFIDT
                     AGIRETKDVVESIGIKKTFEKIEQAQVVLLLVDSSQLSANSLPNVKIEIEKIKNKFPI
                     KPLVIVLNKVDVLDKDSLNNYLNQLSTSNPTTDNRQLITISAKQKTGIDKLKNTLLSF
                     VNTGALRNNETIVTNSRHYDSLLKALEEIQKVQFGLSSNLSSDLMAIDIKQALYYFGE
                     ITGEVTNDELLGNIFANFCIGK"
     gene            complement(6167..9136)
                     /gene="secDF"
                     /locus_tag="PK218_07760"
     CDS             complement(6167..9136)
                     /gene="secDF"
                     /locus_tag="PK218_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HPW98440.1"
                     /translation="MQNKGLVKFFAILFALVSIYQLSFTFVANKVKNDAKAFAGENSE
                     KEVRYLDSIGKEDVFNLGFTKFTYNEVKDKQINKGLDLEGGINVILQISVKDVLVGLS
                     NNSKNPAFNKSLEDATRNQRGNQSYLDAFFEAFEANKGDGKLASPDIFANRNFDDKLV
                     NFKSTDAEVQKALRMKVDESVESAFGVLRSRIDKFGVTQPNIAKLGQTGRILIELPGA
                     KDVDRIKKLVSSKAELEFWETYKAEDFYQFLSSANEALKATVKSEVAEVEKPKDSIAA
                     LLTDKASDSTATKKGNNPIFDKLVSQGGGPVLGVFNAKDVEAIDGYLKRPEIRALVPA
                     DKSNVKFAWGKPNYSTDPKTKKETETVELYVLKGNREDVAPLSGGVIVDARDTFDQLG
                     KPAVSMQMNGQGAKVWEEITGRAYTQKTYIAIALDDVVYSAPGVSTGPISGGRSEISG
                     SFTVAETKDLANVLRAGKLPATAEIVQSEVVGPSLGQKAIDAGTTSSLVGFLLVCLWM
                     VFYYGRAGWYANAALLVNLLFLFGILASLGAVLTLPGIAGIVLTLGTAVDANIIIYER
                     AKEELREGKSLSEAVNSAYGWKGGMRSIIDANVTHILTGAILFIFGTGPIQGFATTLL
                     IGIFTSLFTSIFIARMFIDKDIEANRSLMFATNITKNWFTGFHFDFIGMRKWSYIFST
                     AVVVVSCASFYVNGLDEGVDFVGGRTFQVKFEKPVEATQISDELSAEFGTAVEAKVFG
                     DDDQLRLTTKYKIKEDGVQVDQEVNQKLYNVLKKYFNADVTYEKFINTYDGKKVGVLQ
                     ASKVGAAISADIKTNSFWAVIGAMLVVGLYLVISFRKWQYSLGAIAAVLHDVIFVLGL
                     YSLLYKYMPFHMEMDQHFIAAILTVIGYSMNDTVIVFDRVREFLAGNTKGHFKDVVNA
                     SINTTLSRTINTSFTMILVLLIMFIFGGESIRGFIFAMLVGIVVGTYSSLFIATPVVY
                     DSMDKKNIEDIEARHYK"
     gene            complement(9361..10293)
                     /locus_tag="PK218_07765"
     CDS             complement(9361..10293)
                     /locus_tag="PK218_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPW98441.1"
                     /translation="MKVTIVGAGNVGASCADVISYRGIASEVVILDIKEGFAEGKAMD
                     LMQCATNTGFNTEITGVTNDYSKTANSEVVVITSGIPRKPGMTREELIGINAGIVKSV
                     SENLLKHSPNATFVIVSNPMDTMTYLAFKSLGLPKNKIIGMGGALDSSRFRYYLSKAL
                     NKPANDVSAMVIGGHGDTTMIPLTRLASYNGIPVSQFLSQEELDKVAASTMVGGATLT
                     GLLGTSAWYAPGASVAYLVDSILNNQKKMIACSVFLEGEYGQSDICIGVPCIIGKNGV
                     ESIIDIKLNETEKTLFAKSADAVRSMNNDLKSHL"
     gene            complement(10329..>11956)
                     /locus_tag="PK218_07770"
     CDS             complement(10329..>11956)
                     /locus_tag="PK218_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HPW98442.1"
                     /translation="GKFDKDSYKVSGGLHGVGVSCVNALSDHLRATVFREGVIWEQEY
                     ERGKAMYPVKQIGTTDKRGTTVTFKPDATIFTQTLVYSYDTLASRMRELSFLNKGITI
                     NFTDRREVKEDGSFAGEVFHSQEGLKEFVRFLDGNRVPIISHVISMEHDKSEIPVEVA
                     LIYNDSYAENIFSYVNNINTHEGGTHLQGFRMGLTRTLKKYADASGLLEKLKFEITGD
                     DFREGLTAIISVKVQEPQFEGQTKTKLGNREVVSPVSQAVAEMLENYLEENPNDAKII
                     VQKVILAAQARHAAKKAREMVQRKTVLGGGGLPGKLSDCSEQDPAKCEVYLVEGDSAG
                     GTAKQGRDRNFQAILPLRGKILNVEKAMQHKVFENEEIRNIFTALGVTIGTEEDSKAL
                     NLEKLRYHKVIIMCDADVDGSHISTLILTFFFRFMKELIENGHVYIAAPPLYLVKKGN
                     KKEYAWNDIQRDQANERMGGSAAIQRYKGLGEMNAEQLWETTMDPNFRTLRQVTIDSL
                     TEADRIFSMLMGDEVPPRREFIEKNAVYAKIDA"
BASE COUNT         3775 a         2181 c         1825 g         4175 t
ORIGIN      
        1 ctgtaatggt atattcatca ttgtttttac atgaaatggc aacaattgct attaccaata
       61 aaagagctat ttttttcatt attttgtttt tatttttaac gtgtgcaaat gtatttattt
      121 ttatgtaaca acaactttat tttataataa ctttatctat taggcgaagt ttttgataga
      181 ttagttttac tttaaaaaaa tatttgtagt gttttatttt ggattatata caaatcatat
      241 taaataagcg ttaattgtct attattatta ggaatattgg tttatatctc aataaatttg
      301 aggttcaaaa aaacaaataa tgtttaaaaa attacttcaa attacctgtt ttttcgctgt
      361 tctttccacc tattcgcaag tagttattaa tgaaatagat gcagatactc ctggtactga
      421 cacaaaagag ttcattgaat taaaatcggt taccgctaat ttttctttag atggttatgt
      481 tttggttttt ttaaatggtg gaagtactgg cacctcaaat gcaagctatt tagctttaga
      541 tttagatgga tttacaactg atataaatgg attatttttg ataggaaaca atcaagtttc
      601 acctactcca acctacataa ttccaaatgc atcaatacaa aatggaccag atgttgtagc
      661 tttatatcaa gcaaatgcta gcgattttcc attgaatact attggaactt cttcaaactt
      721 gattgatgca gtagcctatt cttcttctac atcttcttcg ccaacagcta taatgtcggc
      781 tttaggaatt acaacttttg gaatagacaa tacaaatagt agtgccaata catctattca
      841 aagaaataat gatggtactt atacttctgc tacaccaaca cctggccaaa ataacgatgg
      901 aagtggtgtt gttttaaacc atgtaaccat aacagcatcg gcaacaactg ttaatgaggg
      961 tgatacaatc gtttttactt tcactaccga aacacctgtt gttggaagta atctcattat
     1021 caatatggta gttaataatg gaacgtttaa tttacaagac atcaatggaa ctttaactag
     1081 ctttatacct gtgggtcaaa cttctatttc caatagttta ttgattgtta atgatggtat
     1141 aaatgaagga gacgaagaac ttttattaaa tgtacaatca cttcaacctg gatttgtatt
     1201 aaataataat tatctcttaa taagaataaa cgaaagtaat tttgaggtaa aaccatgggg
     1261 aacacctgca aatcctactt atggagtagt tgcaccaact tatccagcag gatactatag
     1321 ctcgctagaa ggattgtcgg gagctgcact taaacaagca ttacaaaata ttattgctaa
     1381 tcctcaagtg gttagagcac atacatacgc cgatatttgg gaaatattaa aaactgccga
     1441 tcaaaatcca gaaaacagta gtcaagtttg gttaatttat aatgaaacgc cacgttcaaa
     1501 attagaccaa caagcaacga gtagtattgt gggtaaatgg aatagagaac atatttattg
     1561 tcaatcaaga ggaaattttg gcgattggta tgacaccagt gcaactggaa taaataatta
     1621 tgttttgtca aatgcaggtg atattgcagc aggattgtct gatgcacatc atttgagagc
     1681 tgctgatggt caagaaaatt cttcaagagg aaacagaaat tacggtgtgg attataacgg
     1741 tccaacagga aatccaggtt cttggaaagg tgatgtagca cgagcattgt tttatatggc
     1801 agttcgctat aatggcttga atgtagtgaa cggaaatcca gttgataaca ctattggaca
     1861 aattggcgat ttaaccactt tgctgtcatg gaatcaacaa gacacatcgg atgattttga
     1921 aatgaacaga aataattata tttatacttg gcaaatgaac cgtaatccct ttattgatta
     1981 tcctagttta gtcaattatg tttttggtgc caactatggt caaccttggt tttcgtcgtt
     2041 agcaacaact gaaataaatt ttgataagat tgttttatat cccaatcctg ttaacgatta
     2101 tctttcaatt attggattaa atacaaaagg aacccttgaa ctatttaata ctcttggtga
     2161 aaaagtattt caaagtgata tcgaaaatca acatcaaatt aatttaaatc ttcaaagtgg
     2221 cgtctatttt gccaaaatat ctagtgaaaa caaaaacttt acaaaaaaaa taattattac
     2281 caattaggtt gtatatgaac gcaggtttta ataaaaaaga atcctcaatt gcactaaaag
     2341 cagagcttat attcaaactc acacaaggat taattgaaat tgttcccaaa gacaacaaat
     2401 tatttttaca agaaacaact gttcgattta tgttggaaga tgcgatgatg attcctgcaa
     2461 aaatttcggg agcagaagct attgatttgt atgatataaa aatggaaaat gctactatca
     2521 ttcgaaaatg tgctcaacaa ttatatgtaa atgctggaaa tctgcgtttt gaagaaagta
     2581 ttaaggacag tgaatacata gaattattgc gagatgaaat tgaagaattt cgcttacttt
     2641 ttattgattg ggttgcctct tttgaccaat ggaattatat aattgatcgc tggggtttat
     2701 ttaatccgcc tggagttact gcgcatgata aagatcctga tgattcaatt tcgtttaact
     2761 caaaagagta ttttgatggt ttggatttag ataatttgga cgaggacgaa aatgatgatg
     2821 ataagtaaat tttttttaaa tcaaattcaa atataatttt ctaagttttt ttgcagcctg
     2881 ctttttatgg tttcttgtaa atgtattggt gctattattt tcaaggtttc tccataagac
     2941 aacaacaaat tttccaactc gtaattgata atcaactcaa tagaaaacaa atagccgttt
     3001 tcatcatcac taattttttt ttgcgaaccg tgaatgggtt ttgttgttag ataaggtttc
     3061 aattcgggag ttgctaataa aattattttt tcgattaatg catcttttgg ttttaaaatt
     3121 ccaataacat cttcaaaata ttcattaaaa tcaacagttg ttgaaatgta ttttgcattc
     3181 gtttcttgaa ccgaaatcat tctgtctata gcaaaatgag taaggttttc atacttttca
     3241 aaacggccaa tcaaaaacca gcgattgttg tattgtttca aataatatgg gtgaatttca
     3301 tgtattttac tctcagcatt attgaatttt tggtattcta tttgcaccac tttttcatat
     3361 aaaatagcat taaataagtc gcctattaaa tgaatgtttt tcaaataaat attagcatca
     3421 aagcttataa tttctttcgt atggctttgc aaattaaatg cttgctgtag ttttaaaatc
     3481 aattcgttaa cccattcaaa ttgcggcatt cctttgaaac gcgccaaaac caataatgcc
     3541 gattttagtt tctctgcctc aaaatcattg atgggttgat tgttgataga aaaatcttta
     3601 tcagcatagc gataataaac cgttctgcct tctttggtgc gctcaagcgg aatttcccaa
     3661 ccttgactac tttccataaa cttaatatct tcaaacagtt gccgtttttt gatgccttca
     3721 ctctgataat tatattctgc taaagcctcg ttacaaacct caagcaaatc ttcgtttgaa
     3781 aatcgtcttc caaaattgct aaagcattta tctaaagcat gataccgaat aagtgcgttt
     3841 ttattaattg ccatttttaa tcgtttacat tatgtgcgcc acaaaaatag cttaaatgaa
     3901 tgcacatcta atgcattaat acaaaatagt tttgtatcat cattttaaaa caagaaatca
     3961 tgagagaaca tttagcccat tttgatattg caggctttac atattatgaa ggtgttttgg
     4021 cattttcaga attagaaata ggaacgcttt tgcaactaaa attagaagaa gataacaaat
     4081 ttgatgctag agcagttgca ctatatttta aaaatttcaa actaggattt gtaccacgaa
     4141 gcgaaaaccg cattttttat aaatttctaa aagtgggtat ttcggattgt tttgaagtta
     4201 gaattcaacg cattgataaa aaagaacatc cagaaaacca aattggagtt atcgtgcatt
     4261 tagtgagaaa agtagcccaa gagtaatact tttggcgata ctttttttag tttttgtttg
     4321 catggtgcag gtatgttttt ataatttttg catgtactct tttgttttct aataaaaccg
     4381 tgatttattt ttctgtcaat gtattacctt caatttctat taagactgaa tggttttaat
     4441 tcggtttttc tttccgtaat tcggcagcag gtttatataa aggagtagcg ttaatttctc
     4501 ctatcttttc caaaatagaa gccactaacg ctacaatttt tttccgttat cgtgtatggt
     4561 ggtttcatcc gttatttatc aattttatta tactgtcgga tgcaatctcg tatacatggt
     4621 ttcatttgtg atagcatcat tgatagcatc aaaaaagaga agtgtaatta cttcccgata
     4681 caaaaatttg caaaaatatt tcccagtaat tcatcattcg tcacttcacc agtaatttct
     4741 ccgaagtagt ataacgcttg tttgatatcg attgccatta agtcggacga taaattggaa
     4801 cttagtccaa attgcacttt ctgaatttct tctagtgctt taagtaaaga atcatagtgg
     4861 cgagaattgg ttactatggt ttcgttattt cgcaatgcac ctgtgtttac gaaagacaat
     4921 aacgtgtttt ttaatttgtc gattcctgtt ttttgttttg ctgagattgt tattagttgt
     4981 cggttatcag ttgtcggatt agaagttgac agttgattta ggtaattgtt taaactatct
     5041 ttatctaaaa catcaacttt attcaaaacg ataactagtg gttttattgg aaatttgttc
     5101 ttgattttct caatctcgat tttcacattt ggtaagctgt tggctgacaa ctgagaactg
     5161 tcaactaaaa gcaacaccac ctgagcttgc tcaatcttct caaaagtttt tttgatacca
     5221 atgctttcta ctacatcttt ggtttcacga attccggccg tatcaataaa tcggaaaccg
     5281 attccatcaa taaccaattc gtcttcaatg gtatcacgag ttgttccagc aatatcagaa
     5341 actattgcac gttcttcgtt tagtaaggca ttcaataaag ttgatttccc aacattaggc
     5401 tcgccaacaa tagccactgg aattccgttt ttaagaacgt ttcctatggc aaaagaatct
     5461 attaatcgtt ttaaaacaaa ctctattcgg gttactaatt ctttaaattg tgttctgtcg
     5521 gcaaattcta catcttcttc tgcaaaatcc aattcgagtt caattaacga tgcaaagttc
     5581 aataactctt gacgcagctg tgcaatttcg ttactaaaac cgccacgcat ttgctgcatc
     5641 gcaatttggt gactggcttc attatctgaa gaaattaaat cggcaacggc ttcggcttgt
     5701 gataaatcta attttccatt tagaaaagct ctaagtgtaa attctccagc ttgtgccatt
     5761 ttacaacctt ttcgcaataa taattggata atttgttgtt ggataaaagt tgaaccatga
     5821 caagatattt caactacatc ttcacctgta tatgaatttg gatttttaaa aacagaaacc
     5881 aacacttgat cataaatttt ggtattctca acaatatgcc ctagatgaat ggtatgtgat
     5941 ttttgtttgg acaaatcttt tccagaaact gattgaaata ctttggatgc aacagaaatc
     6001 gcttcattac ccgataatct aatgatggca atggctccag caccagatgg tgttgctaaa
     6061 gctacaatag tttcttgatt aatcatttta actttttttc aaaattatta ataaaaaaaa
     6121 ggttgaacaa gttcaacctt ttttaattta tacaactaca ctaaaattat ttatagtgtc
     6181 ttgcttcaat gtcttcaatg ttctttttat ccatcgaatc ataaacaact ggagttgcta
     6241 tgaataaaga agaataagtt cctactacaa taccaaccaa catggcaaag ataaatcctc
     6301 tgattgattc tccaccaaag atgaacatga ttaataatac taatatcatt gtaaatgatg
     6361 tattgatagt tcttgataaa gtagtgttaa tagaagcgtt tactacatct ttaaaatgtc
     6421 ctttagtgtt tccagcaagg aactctctaa cacggtcaaa tacaattaca gtatcattca
     6481 ttgagtaacc aattacggtt aggattgccg caataaagtg ttggtccatt tccatgtgga
     6541 aaggcatata tttatataac aaagagtata aacctaatac gaaaataaca tcgtgcagta
     6601 cagctgcaat tgctcctaat gaatattgcc atttacggaa cgatattact agataaagcc
     6661 caacaactaa catagcacca ataactgccc agaacgagtt tgttttaata tctgctgata
     6721 tagcagcacc aactttagat gcttgaagta cacctacttt tttgccgtca taggtattga
     6781 taaatttttc gtaagtaaca tcagcattaa aatacttttt caatacattg taaagttttt
     6841 gatttacttc ttgatctact tgaacgccgt cttctttaat tttatattta gtcgtaagtc
     6901 ttaattgatc atcatcacca aatacttttg cttcaacagc agtaccgaat tcagctgata
     6961 actcatctga aatttgtgta gcttctactg gtttttcaaa tttcacttgg aaagttcttc
     7021 ctccaacaaa atcaacacct tcgtctaatc cattaacgta aaaagaagca caactaacaa
     7081 caacaactgc tgtagagaat atgtatgacc attttctcat tccgataaaa tcaaaatgga
     7141 aacctgtaaa ccaattttta gtgatattgg ttgcaaacat taaacttcgg ttcgcttcga
     7201 tatctttatc aataaacata cgagcgatga agattgatgt aaataacgat gtgaaaatac
     7261 caattaataa agtagttgca aatccttgaa ttggtcctgt accaaagatg aataagattg
     7321 cacccgttag aatgtgtgta acgtttgcat caatgatcga acgcatacca cctttccaac
     7381 cgtatgctga attaacagct tcgctaagag attttccttc acgaagttct tcttttgctc
     7441 tttcatagat gataatattc gcatctaccg cagtaccgag tgttaacaca ataccagcaa
     7501 tacctggtaa tgttaataca gcacctaaac ttgctaaaat tccgaataag aatagtaagt
     7561 taactaataa agctgcattg gcataccaac ctgctctacc ataataaaat accatccata
     7621 aacatactaa caagaaacca acaagtgatg aagtagtacc cgcatcaata gctttttgtc
     7681 ccaatgatgg tccaaccact tcggattgta caatttctgc tgtagctggt aatttaccag
     7741 ctcttaatac gttagctaaa tctttagttt ctgcaacggt aaaactaccc gatatttcac
     7801 ttcttcctcc cgaaattggt cctgtagaaa cacctggtgc tgaataaaca acatcatcca
     7861 atgcaatagc tatgtatgtt ttttgtgtat atgctctacc tgtaatttct tcccatactt
     7921 tagcaccttg accgttcatt tgcattgaaa cagctggttt tcctaattgg tcgaaagtgt
     7981 ctctcgcatc aacaattaca cctccactta atggagctac atcttctcta tttcctttta
     8041 aaacgtataa ttcaaccgtt tcagtctctt ttttagtttt tgggtctgta ctataatttg
     8101 gttttcccca agcaaatttt acatttgatt tatctgcagg tactaaagct ctaatttcag
     8161 gtctttttaa atatccatct attgcttcaa catcttttgc attaaaaaca cctaaaacag
     8221 gtccaccgcc ttgagaaaca agtttatcaa atattggatt atttcctttc ttggtagccg
     8281 ttgaatctga tgctttgtca gttaaaagcg cagcaataga atcttttggt ttttcaactt
     8341 cagcaacttc agatttaaca gtagctttaa gtgcttcgtt tgcggaactt aagaattgat
     8401 aaaaatcttc tgctttatag gtttcccaaa attctaattc cgctttgctt gaaactaatt
     8461 ttttaatacg atccacatct tttgcgcctg gcaattcgat taggattctt cctgtttgtc
     8521 ctaattttgc aatgtttggt tgcgttacac caaatttatc aatacgactt cttaatactc
     8581 caaaagcaga ctcaaccgat tcgtcaactt tcattcttaa tgctttttgt acttcagcat
     8641 ctgtagattt aaagtttact agtttgtcgt caaaatttct attagcaaaa atgtctggag
     8701 aagctagttt tccatcacct ttattggctt caaaagcttc aaaaaaagca tccaaataac
     8761 tttgatttcc tctttgattt ctcgttgcgt cttctaatga tttgttaaat gcaggatttt
     8821 tagaattatt tgacaatcca accaatacat ctttaaccga aatttgaaga attacattga
     8881 ttcctccttc aaggtcaaga cctttgttga tttgtttgtc ttttacttca ttataagtaa
     8941 attttgtaaa tcccaaatta aagacatctt ctttcccgat tgaatcaaga tatctaactt
     9001 ctttttctga attttcgcct gcaaatgctt tggcatcatt ttttacctta ttggctacaa
     9061 aagtgaatga aagttggtaa atacttacca atgcaaatag aattgcgaaa aacttaacta
     9121 gtcctttatt ctgcattatt actaaaaatt aattaatttt ttgtttttgg ttaaaatttt
     9181 aaaactgaaa accgttgaag ttcatagcct tactgataaa agcaatgatt tcaagaagtt
     9241 tccttttttt tgaaacgtgc aaatatataa ttctcaatac gattaagcaa tatatttgtt
     9301 aattaatata aaaaaaatga ctgcctatta gcagccattt tagaatattc aaattttatt
     9361 ttataaatgt gatttcaaat cgttgttcat gctgcgaaca gcatcagcac tttttgcgaa
     9421 taatgttttt tcagtttcat ttaacttaat atcaatgatt gactctactc cattttttcc
     9481 aatgatacat ggaacaccaa tacaaatatc gctttgaccg tattcgcctt ctaaaaatac
     9541 cgaacaagca atcattttct tttgattatt taaaatacta tccaccaaat aagcaaccga
     9601 tgcgccagga gcataccaag ccgaagttcc taataagcct gttaaagtag ctccaccaac
     9661 catagtactt gctgcaactt tatctaactc ttcttgcgac aagaactgag aaactggaat
     9721 tccattgtaa gaagctaatc ttgttaaagg aatcatagtt gtatcgccat gaccgccaat
     9781 aaccatggct gaaacatcat tagcaggttt gtttaacgct tttgacaaat aatatctaaa
     9841 acgagaacta tctaaagcac cgcccattcc gatgatttta ttttttggta agcccaaaga
     9901 tttaaatgct aaatatgtca tagtatccat aggattagac acaattacga aagtagcatt
     9961 tggcgaatgt tttaaaagat tttcagaaac tgactttaca attccagcgt ttatcccgat
    10021 taattcttca cgggtcattc ctggttttct tggtattcct gaagtgataa ccaccacttc
    10081 gctatttgct gtttttgaat aatcattggt aactcctgtg atttcggtat taaacccagt
    10141 attagtcgca cattgcatca aatccatcgc tttaccctct gcaaaacctt ctttgatatc
    10201 taatattact acttcactag caattcctct ataagagata acatctgcac aagaagctcc
    10261 tacgtttcct gctcctacta ttgtaacttt cattatttta tatatttatt tgtgtttgtt
    10321 ttattttatt aagcatcgat tttcgcataa actgcatttt tctcgataaa ctctcttcgt
    10381 ggcggcactt cgtcacccat caacattgag aaaattctat ccgcctccgt caaactatcg
    10441 atagtaacct gacgtaaagt tctaaagttt ggatccattg tcgtttccca aagttgctcc
    10501 gcattcatct ctccaagacc tttatatcgc tggatagcag cacttcctcc catgcgctca
    10561 ttggcctgat cacgctgaat atcattccat gcatattctt ttttgtttcc tttttttacc
    10621 aaatacaatg gcggcgcagc aatataaaca tgtccgttct ctattaactc tttcataaat
    10681 cggaagaaga aagttaatat taaggtagaa atgtgactac catcgacatc ggcatcacac
    10741 attataatta ctttatggta acgcagtttt tctaaattca atgcctttga atcttcttct
    10801 gttcctatgg taactcctaa tgccgtgaaa atattacgga tttcttcgtt ttcaaaaact
    10861 ttatgttgca ttgctttttc tacgttcaaa atctttcctc gcaaaggtaa aatagcttgg
    10921 aaatttcgat cgcggccttg ttttgctgtt ccacctgccg aatctccctc aacaagatat
    10981 acttcgcatt ttgctggatc ttgctcggaa caatccgata atttccctgg taaacctcca
    11041 ccgcctaaaa cggttttgcg ctgcaccatt tcacgcgctt tctttgcagc atgacgcgct
    11101 tgcgcagcca agattacttt ttgaacaatg atttttgcat catttggatt ttcctccaaa
    11161 taattttcaa gcatttccgc taccgcttgc gaaactggag aaactacttc tctatttcct
    11221 aatttagttt tggtttgacc ttcaaattgc ggctcttgca cttttaccga aataattgcc
    11281 gtcaatcctt cacggaaatc atcacccgta atttcgaatt ttaatttctc taacaaccca
    11341 gaagcatctg catatttctt taatgtacga gtcaacccca tacggaaacc ttgcaagtgt
    11401 gttcctcctt cgtgcgtatt gatattgttt acatacgaaa aaatattttc tgcataacta
    11461 tcgttataaa ttaaagcaac ttcgactggt atttcgcttt tatcatgctc cattgaaata
    11521 acatgcgaaa taatcggcac gcggtttcca tctaaaaagc gaacaaactc ttttaatcct
    11581 tcttgagaat gaaacacttc acccgcaaaa cttccatctt ctttaacttc tcttctgtcc
    11641 gtaaaattga tagtaattcc tttattcaag aaagacaact ctcgcattct tgacgctaat
    11701 gtatcataag aatacactaa tgtctgagta aagattgtgg catccggttt aaaagtaacc
    11761 gttgttcctc ttttatcggt ggttccgatt tgttttacag gatacatcgc ctttccgcgc
    11821 tcgtattctt gctcccaaat aacaccttca cggaaaactg ttgcacgcaa atgatctgat
    11881 aatgcattca cacacgaaac tcctactccg tgcaatcctc ctgatacttt atacgaatct
    11941 ttatcaaatt ttcctc
//
LOCUS       DAOOBH010000068        11878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_6885_length_11878_cov_7.320477, whole genome shotgun sequence.
ACCESSION   DAOOBH010000068 DAOOBH010000000
VERSION     DAOOBH010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11878
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6885_length_11878_cov_7.320477"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /gene="metG"
                     /locus_tag="PK218_07775"
     CDS             <1..675
                     /gene="metG"
                     /locus_tag="PK218_07775"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HPW98443.1"
                     /translation="KYLADEEPWKMVKENPERVKTQMFVALQIASALAILSEPFLPFT
                     AKKLCNILNIKPNHWNLEFENWNLVKAGHQINPAEILFAQIEDAEIQKQIDKLEATKT
                     ANAMENKVVEPQKETSTFDDFTKLDLRVGTILEAEKMPKANKLLVLKVDTGIDTRTIV
                     SGIAEHFSPEEVVGKRVTVLVNLAPRALRGVESQGMILMTNDASGKLVFVNPDADGVE
                     NGATIS"
     gene            698..1585
                     /locus_tag="PK218_07780"
     CDS             698..1585
                     /locus_tag="PK218_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPW98444.1"
                     /translation="MYYRRKIILSLLQVFDGELEKIQLQKLLFLFTRFQKDKKTYDFV
                     PYKFGCYSFQANADLSTLKKYGIVSETTKSWKKEDEVNYLTELTKEDKKTISDFRIIY
                     ANKTSDDLINLTYKRYPYFAINSTVAKEYLTEEDFKNLDNYRSFEEDITLFTIGYQGI
                     TLEAYLNKLIKNNIKVLCDVRKNALSMKYGFSKSQLKNACNGVGIEYIHIPEVGIDSE
                     QRQELKTQTDYDVLFEEYKNLNLKKSINSQTEILNILKKNRRIALTCFEADICQCHRK
                     HLSEAITNLKGFKYTLEHL"
     gene            1589..2404
                     /locus_tag="PK218_07785"
     CDS             1589..2404
                     /locus_tag="PK218_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98445.1"
                     /translation="MPKKRILISVTTYPLPSRSYDELVCTAGFLEDGTWIRIYPVPLS
                     FLKEVKKNTNFKKYTWIELDVKQRTDDFRPESHSPNNYDIKIINTLNTDNFWQERKEI
                     CLKNIYTNKSKLLEDSKAPKNTSLVTFKPSKITDFIIEDDEKEWKDEWKEIRKQTDLF
                     AEIETDPEKLIPKVPYKFYYVFEDDEGVPSKLMIEDWEIFQLYLNCLKDNNNNQEIAI
                     QKVKEKYFDVFTKENDIYFFLGTTMKWHRQRARNPFVIIGVFYPKIKYQYGLF"
     gene            2404..3087
                     /locus_tag="PK218_07790"
     CDS             2404..3087
                     /locus_tag="PK218_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPW98446.1"
                     /translation="MTKKIIAFDADDTLWHNEPYFDEAQERFAELFQDYASKQEILQL
                     ILSHQVKNLPLYGFGIKAFTLSMIDSALELTNNKISGDGIQKIITIGRDLLQKPVELL
                     PYVEEVLATLYGKYKLVVATKGDLKDQHRKLHDSGIGHYFHHIEVLSDKKELDYQKML
                     QRLEINPTDFIMIGNSLKSDVLPILNIGGHGIHIPYHTTWEYEKIDFEITHENFKALE
                     NIKQVVDLF"
     gene            3178..4575
                     /locus_tag="PK218_07795"
     CDS             3178..4575
                     /locus_tag="PK218_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98447.1"
                     /translation="MKNIYLFLFSFISLISFSQIKLTSFPIELKRSAENHQMMNFVDK
                     NSNEIYTFVADKETVIGVKFNSAVFVSDSIATSKPFNFQYIIGYSFSKENNPTVHWIT
                     EDNKKIMSIEYDFSKNPKDYTATETIFSNESILASFSNNGVFYIITDNDKNDMKLYEF
                     GTQNNTTTLPSGNLNFTDSKDKPIKISQVFIENPLVRMETNFYNPLMATAEKVKFYVE
                     NNRAIFTFDLNRSKTEILEIDLNSKTISKKEYQQSTLENGSNQSNSYYLNEKLFQVKS
                     NEKQLLLRITDCYSKTVIKEYSLSEKENPFEYSSFLIQTEQYKPETLSSLKRFLRKIE
                     NSNLAFSIFQINEGYVASFGANKTSASSGGLAFGIGVGIGSILASGTFVDVGNFDNSR
                     TQSVFLDFDCDANFNIIPPKNTVLATSIISGFIAQNDNIRLYQTFPYRDYFVLSYYDK
                     KAKQVVLYKFQNGFN"
     gene            complement(4568..5011)
                     /locus_tag="PK218_07800"
     CDS             complement(4568..5011)
                     /locus_tag="PK218_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98448.1"
                     /translation="MNKIKFLFLLFLVLYASSNCFAQVGINTVMPQSTLDINGNLSVK
                     VINITGNGTGSVGTSVVINDGIYISINPTATDDKFELPNPTLVPGRMYIIRNINNTVT
                     AQLITTTGLLFPKNSTTGSSQIYMYEGNLRTVTVFSDGNNWTYLN"
     gene            complement(5172..5612)
                     /locus_tag="PK218_07805"
     CDS             complement(5172..5612)
                     /locus_tag="PK218_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HPW98449.1"
                     /translation="MNIRVFNIRLSKEFCIIDQNRMNEFLDTVEVKLSSTNFVTNGTV
                     DYWSAVVFYEPKKVTKEKNEVAFSIDDLSIEEQKIFKALRSWRNDLAEKLGWSSFRIC
                     HNSHLIHIAKENPTSLNDLGKITGFGTVRAEKYGHDILAVLNAY"
     gene            complement(5681..6013)
                     /gene="ssb"
                     /locus_tag="PK218_07810"
     CDS             complement(5681..6013)
                     /gene="ssb"
                     /locus_tag="PK218_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPW98450.1"
                     /translation="MNAMKNKVQLIGHVGQEPEVKIFGEGKKVVNLSLATNESYTNSK
                     GEKVEDTQWHRVKAWGKVAEIIEKYVTKGKEIAIEGKLTYSDYVDKDGVKRYLTEVVA
                     NDVLLLGK"
     gene            6261..7784
                     /locus_tag="PK218_07815"
     CDS             6261..7784
                     /locus_tag="PK218_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HPW98451.1"
                     /translation="MQEIKTVELRNLQLEDYKQLKSSMIEAYPGMENSYWKEEQIEKL
                     LTIFPEGQLVIVVDDKVVGSALSLIIDEDFVDKSHDYKKITGNYTFSTHNADSEILYG
                     IDVFIHPNYRGLRLGRRLYDARKELCEQLNLKSIVFAGRIPNYGKYAEELSPKAYIEK
                     VRKKEIHDPVLSFQLSNDFHVMRVMKNYLEGDKKSKEYAVLLEWNNIYYDESPRFINT
                     ERSIIRLGLVQWQMRSLSNLEEFFNQAEFFIDAVSGYESDFALFPELFIAPLMADYNH
                     LSEADAIRELAKYAEPIKKKFQEFAISYNINIISGSMPYLEDGILYNAGFLCKRDGTN
                     EVYKKIHITPNEVFHWGITGGSKIQTFDTDCGKIGVVICYDIEFPELSRLMADEGMNI
                     LFVPFLTDTQNGYTRVKCCAQARAIENECYVAIAGCVGNLPKVNNMDIQYAQAAVFTP
                     SDFAFPSNGIKAEATPNTEMTLIVDVDINLLKELHEHGSVKIMKDRRHDLYSIKKLK"
     gene            7830..8180
                     /locus_tag="PK218_07820"
     CDS             7830..8180
                     /locus_tag="PK218_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98452.1"
                     /translation="MTKNCLECNEKIIGREDKKFCSDGCRNSYNNKINKDSTNYMRNI
                     NNKLRKNYRILSALNIDGKSKTTKSKLLSKGFDFEYMTTILHTKTGNTYHFVYDQGYM
                     VLDNDFYMLVKKDI"
     gene            8191..10605
                     /locus_tag="PK218_07825"
     CDS             8191..10605
                     /locus_tag="PK218_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPW98453.1"
                     /translation="MKNNQPTIIVALLLIGLLSGIFYFMMPQSYDTSEAPLSEFSTKR
                     ALEKVKHISQKPHYVGSQNHVLVANYIKKELQDLGLQTSDQEGFVMTEKGTLVKAKNI
                     LAKIKGTSSALSETNNSKSLVLLSHYDSAPHSYSLGASDDASGVATIIESVRGFLHNK
                     TKHKNDIIILFTDAEEIGLNGAALFTTQHQWAKEVGLVLNFEARGSSGPSYMLMETNK
                     GNSKMVDAFKAGKAPWSVSNSLMYSIYKMLPNDTDLTVFREVKQIQGFNFAFIDSHYN
                     YHTSRDNYQNLSRETLAHQGSYLFPLLTYFSNADLSNLNATNDQVYFNIPFSFVSYPF
                     SWILPMLLITFGLFLLFVFVGLGKRALLLDEVFKGFKPFFGALITSGAVTYIGWKLLL
                     NFYPQYNDILHGFTYNGHDYIYAFVSLTLGICFLFYKKQAKHNTAMNQLFAPLLIWLL
                     LNTAIAFKLEGAGFLIIPVICSTIMLAVYVFTQRSYWVLNLILSIPALLLLVPFIMMF
                     PIGLGLKILFGSAVLTVLAFTLLLPIFGNFEKKNLWSILFFLLSIGLFIKAHQISEFS
                     TERPKPNSLVYLLNADTQKAYWATYDKNLDEWTKGYLGEKPADAKILNTNKLYSKYGS
                     SYTYMAEAPIKNISSPNIEFLTDSIIGSQHVYKIKISPNRNVNRYDVFVKNGITINSL
                     IANGVKSIDFKSNIASKTSGKILSYYVVENIPLELEFSIKANDKLDLELLESSFDLMS
                     NTDFTITKRKSWMIPTPFVLTDAVIIRQKVKPSTHSSLRTPRIALPSRRYTSPDSLNV
                     VMDSLK"
     gene            10605..11408
                     /locus_tag="PK218_07830"
     CDS             10605..11408
                     /locus_tag="PK218_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPW98454.1"
                     /translation="MTQISILGCGWLGLPLAKSLIEKGFWVNGSTTSLEKIAVLKNEG
                     ITAFQIEVTENEIIGNLENFLKNSAILILDIPPKLRGNQPENFVKKIKNILSFVENSS
                     VQKVLFISSTSVYADDNSKINEETKPQPETESGKQLLEVENLLKANSNFQTTIIRFGG
                     LIGDDRNPIHFLAGRKNIQNPDAPINIIHLKDCIGVIESVIEKNIWNETFNAVCPFHP
                     TRKDYYTQKAIALNLPIPEFDFSNKSIGKIISSTKTETVLNYTFQKTNL"
     gene            11405..11656
                     /locus_tag="PK218_07835"
     CDS             11405..11656
                     /locus_tag="PK218_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98455.1"
                     /translation="MNFNDLIGTIGVGIILLAYFLNIFSLIKKDGILFYLMNIIGASI
                     ACFASVLISYMPFIILEATWAIVSVIGLLKVIKNAPNSD"
     gene            complement(11703..>11878)
                     /locus_tag="PK218_07840"
     CDS             complement(11703..>11878)
                     /locus_tag="PK218_07840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HPW98456.1"
                     /translation="SKMRDEAIKNQVKTDPHSPGMYRGYVPLLNLETFHQAFGIKPGD
                     GMYVAPEKRVLIW"
BASE COUNT         4203 a         1812 c         1905 g         3958 t
ORIGIN      
        1 aaatatttag ccgacgaaga accatggaaa atggtaaaag aaaatccaga acgtgtaaaa
       61 acacaaatgt ttgtggcatt acaaattgct tcggctttag ccattttatc ggaaccattt
      121 ttacctttta cggctaaaaa attgtgcaac attttaaaca taaaaccaaa ccattggaat
      181 ttggaatttg aaaattggaa tttagtaaaa gctggacatc aaattaatcc agccgaaata
      241 ttatttgccc aaattgaaga tgccgaaata caaaaacaaa tagacaaact agaagctacc
      301 aaaaccgcaa acgctatgga aaataaagtg gttgaaccac aaaaagaaac ttcaactttt
      361 gatgatttta ccaaattgga tttacgtgtt ggaaccattt tagaagctga aaaaatgcca
      421 aaagccaaca agcttttagt tttaaaagtt gatactggta ttgacacaag aactatcgtc
      481 tcgggaattg cagagcattt ttcgcctgaa gaagtagttg gaaaacgcgt taccgtttta
      541 gtgaatttgg caccaagagc tttgcgtggc gttgaaagtc aaggaatgat tttaatgacc
      601 aatgatgctt ctggtaaatt ggtttttgta aatccagatg ctgatggtgt tgagaatggt
      661 gcgacaatta gttagaaatt attactttta caaaactatg tattacagaa gaaaaattat
      721 attatcctta cttcaagttt ttgatggtga attagaaaaa attcagcttc aaaaattatt
      781 gtttttattt actcgatttc aaaaagataa aaaaacgtat gattttgttc catataaatt
      841 cggttgttac tcatttcaag caaatgcaga tttatcaact ttaaaaaaat acggaattgt
      901 tagtgaaaca actaaatctt ggaaaaaaga agatgaagtt aattatttaa ctgaactaac
      961 aaaagaagat aaaaaaacaa taagcgattt tagaattatt tacgctaata aaacaagcga
     1021 tgatttaata aatcttactt ataaaagata cccatatttt gctattaaca gcactgtagc
     1081 aaaagaatat ttgacagaag aagactttaa aaacctagat aattatagat cttttgaaga
     1141 agatataact ttattcacta ttggttatca aggaataact ttagaagcat atttaaataa
     1201 attaataaag aataatataa aagttctttg tgatgttaga aaaaatgctt tgagtatgaa
     1261 atatggtttt agtaaaagtc aattaaaaaa tgcttgtaat ggtgttggaa ttgaatacat
     1321 tcatatacct gaagtaggaa ttgattctga acaaagacaa gaattgaaaa ctcaaactga
     1381 ttacgacgtt ttatttgaag agtataaaaa tcttaatctt aaaaagtcaa ttaatagtca
     1441 aactgaaatt cttaatatat tgaaaaagaa tagaagaatt gctttaactt gtttcgaagc
     1501 tgatatttgt caatgtcata gaaaacattt gtccgaagca attacaaatt tgaaaggatt
     1561 taaatatact cttgaacatt tataatttat gccaaaaaaa agaattctaa tatctgttac
     1621 tacatatcca ttaccatcaa gaagttatga tgaactagtt tgtactgctg gatttttaga
     1681 agatggaaca tggataagaa tatatcctgt cccattaagt tttttaaaag aagttaagaa
     1741 aaatacaaat tttaaaaaat atacttggat tgaacttgat gtaaagcaac gaaccgatga
     1801 ttttcgtcct gaaagtcatt caccaaataa ttatgatatt aaaattatca atacactaaa
     1861 tacagataat ttttggcagg aacgaaaaga aatttgttta aaaaatatat acacaaataa
     1921 atccaaatta ttagaagatt caaaagcccc aaaaaacacc tctctagtca catttaaacc
     1981 ttcaaaaata actgatttca ttattgaaga tgatgaaaaa gaatggaaag acgaatggaa
     2041 agagattaga aaacaaactg atttgtttgc tgaaatagaa actgatcctg aaaaattaat
     2101 tccaaaagtt ccttataaat tttattatgt ttttgaagat gatgaaggag taccaagcaa
     2161 attaatgatt gaagattggg agatttttca gctatacttg aattgtttaa aggacaataa
     2221 caataatcaa gaaatagcaa ttcagaaagt aaaagaaaaa tattttgatg tttttactaa
     2281 agagaatgac atttattttt ttctaggaac aacaatgaaa tggcatagac aaagagcaag
     2341 aaatcctttt gtaataattg gtgtatttta ccccaaaata aaatatcaat acggtttatt
     2401 ctgatgacaa aaaaaataat cgctttcgac gcagacgaca cactttggca caacgaacct
     2461 tactttgacg aagcccaaga acgctttgct gaattatttc aggattatgc cagcaaacaa
     2521 gaaattttac agttgatatt aagtcatcaa gtgaagaact tgcctctgta tggtttcgga
     2581 attaaggcgt ttacattgtc tatgattgac tcggcattag aattgaccaa caataaaatt
     2641 tcgggtgatg gtattcaaaa aatcattact attggtagag atttgctaca aaaacctgtc
     2701 gaattattgc cctatgttga agaagtttta gcaactttat acggaaaata caaattagtc
     2761 gttgccacaa aaggcgattt aaaagaccaa caccgaaaac ttcacgactc aggaattggt
     2821 cattattttc atcatataga agtgttgagc gacaaaaaag aattggatta tcaaaaaatg
     2881 ttgcaacgcc tagaaatcaa tcctactgat tttataatga ttggaaactc tctaaaatcg
     2941 gatgttttgc ctattttgaa tatcggcgga catggtattc acattccgta tcacactact
     3001 tgggaatacg aaaaaattga ttttgaaatc actcacgaaa acttcaaagc gttagaaaac
     3061 attaagcaag ttgttgattt attctaaaaa gattaacaaa tttttatcgt cgtaactaaa
     3121 ttttctattt caaaaatccg ttatctttga gaggtaacat ctattgaatt ttacagaatg
     3181 aaaaacatat atctctttct tttcagtttt atatcactaa tctcttttag tcaaatcaag
     3241 ttaacttctt tcccgattga attaaaacgc agtgcagaaa atcatcaaat gatgaatttt
     3301 gttgataaaa acagtaatga aatctacact tttgttgctg ataaagaaac tgttattgga
     3361 gtaaaattta acagtgccgt ttttgtttct gacagtatag caacatcaaa gcctttcaat
     3421 tttcaataca ttattggtta tagtttttca aaagaaaaca atccaacggt gcattggatt
     3481 accgaagata acaaaaaaat aatgtcgatt gaatatgatt tttctaaaaa tccaaaagac
     3541 tacactgcta ccgaaacaat cttttctaac gaatcgattt tagcgtcatt ttccaataat
     3601 ggtgtttttt acatcattac cgataacgat aaaaacgaca tgaaattata tgaatttggc
     3661 actcaaaata acactacaac actaccatca ggtaatttaa atttcacaga ttccaaagac
     3721 aaaccaatta aaatcagtca agtttttatt gaaaacccgt tggtacgcat ggaaactaat
     3781 ttctataatc cgctgatggc aacggcagag aaagtaaaat tttatgtaga aaacaaccgt
     3841 gctattttta cgtttgattt gaaccgttcg aaaactgaaa ttttagaaat tgatttgaat
     3901 tcgaaaacta tttctaaaaa agaataccaa caatctacgc tcgaaaatgg ctctaatcaa
     3961 tcgaattctt attatttgaa tgaaaaatta ttccaagtaa aatcgaatga aaaacaattg
     4021 ttgctgcgca ttaccgattg ctattcgaaa acggttatca aagaatattc gctttcagaa
     4081 aaagaaaatc cttttgagta ttcttctttt ttaattcaaa ccgaacaata caaacccgaa
     4141 accttgagtt cgctaaaaag gtttttgaga aagatagaaa acagcaattt ggccttttca
     4201 atttttcaaa taaacgaagg ttatgttgct agttttggtg ccaacaaaac aagtgctagt
     4261 tctggtggat tagcttttgg tattggcgtt ggtattggtt ctatattggc tagtggtact
     4321 tttgtagatg taggcaattt tgataatagc agaacgcaaa gtgtgttttt agattttgat
     4381 tgtgatgcta attttaacat cattccgccc aaaaacacgg tcttggcaac cagcattatt
     4441 tctggtttta ttgcccaaaa tgacaatatt cgactgtatc aaacctttcc ttatcgcgat
     4501 tattttgtgc taagttatta tgacaaaaaa gccaagcaag ttgttttgta taaatttcaa
     4561 aacggattta attgagatac gtccaattat tcccatcact aaaaactgtt actgttctta
     4621 gatttccttc atacatataa atttgactac ttcctgttgt agagtttttt ggaaaaagca
     4681 aacctgttgt agttatcaat tgtgctgtta cggtattgtt tatatttcta ataatataca
     4741 tccgcccagg tactaatgtt ggattgggta attcaaattt gtcatcggtt gctgttggat
     4801 ttatcgaaat atatattcca tcatttatta ccacagaagt tcctacagaa ccagttccgt
     4861 ttcctgtaat attgattact tttaccgata aattcccgtt aatatctaaa gtgctttgag
     4921 gcatcacagt attgattcca acttgagcga aacaattact tgaagcataa agtactaaaa
     4981 ataaaagaaa taaaaactta attttgttca tgttttacaa tgataccatt tttaaatgta
     5041 cagaaactat ttagaaaatg gctgccaaaa ttatttttaa acccaccgaa aacgtttgcg
     5101 tgttgacaac tttatctttt ttgtgttaaa attttacatt ttttgaacca aaaaaatcaa
     5161 taaaaaaacg attagtaggc atttaaaaca gcaagaatat catgaccata tttttcggct
     5221 cttacggttc caaaaccagt tatctttcct aaatcattca acgacgttgg attttctttt
     5281 gcaatatgaa ttaaatgtga attgtgacaa attctaaaag atgaccaacc taatttttct
     5341 gccaaatcat tacgccaact tcgcaatgct ttaaaaattt tttgctcttc gatagataaa
     5401 tcatcgattg aaaaggcaac ctcatttttt tctttagtaa ctttttttgg ttcataaaaa
     5461 actactgccg accaataatc gaccgttcca ttagttacaa aattagtaga actcaacttt
     5521 acttctacag tatctaaaaa ctcattcatt cggttttgat caataataca aaactctttg
     5581 ctcaaacgaa tattaaaaac tcgaatattc ataatttaaa aattttaatg gattaaaaaa
     5641 tgcacgaact tcaagaaatt cgtgcatttt tatactctgc ttattttcct aataacaaaa
     5701 catcgttggc aacaacttcg gttaaatagc gttttactcc gtctttatca acataatcac
     5761 tgtatgttaa tttaccctca atcgcaattt ctttaccttt tgttacatat ttttcaataa
     5821 tctcggcaac tttaccccaa gcttttactc gatgccattg cgtgtcttct actttttcac
     5881 ctttcgaatt ggtgtaactt tcattagtag ctaaagataa atttactact ttttttcctt
     5941 ctccaaaaat ttttacttct ggctcttgtc ctacgtgtcc aattaattgt actttgtttt
     6001 tcatggcgtt catggcgttt aaattttaaa atggtttata tatttatttt tttgtctagt
     6061 gaaaattgaa caattatttt attgtcattg aatttgaatt ttaaaattgt atttgtcatt
     6121 ctttcatttc gacgatgcaa agttgtgata gtgaacaaaa attattcggt agttatctat
     6181 ttactttcgg ttgtaactat ttgtaaccgt ttgtaaatgg ataatatttt tttatcttta
     6241 ctaaaaatca ataatttaag atgcaagaga ttaaaacagt agaattacga aatttacaac
     6301 tagaagatta caaacaactg aaatcgtcaa tgatagaagc ctatcctggc atggaaaaca
     6361 gctattggaa agaggaacaa atcgaaaaac tgcttactat tttccccgaa ggtcagttgg
     6421 taattgttgt tgatgacaaa gtagttggct cggcgctttc cttaattatt gacgaagact
     6481 ttgtagataa aagtcacgat tataaaaaaa taactgggaa ctacacattc tctactcaca
     6541 acgctgatag cgaaatttta tatggaattg acgtctttat tcacccaaat tataggggtt
     6601 tacgactggg aagacggctt tacgacgcta gaaaagaatt gtgcgaacaa ttaaatttaa
     6661 aatcgattgt ctttgccgga cgtattccta attatggaaa atacgcagag gaactttccc
     6721 caaaagctta tattgaaaaa gttagaaaaa aagaaatcca cgatcccgtt ttgtcgtttc
     6781 aattgagcaa tgatttccat gtgatgcgtg tgatgaaaaa ctatcttgaa ggcgacaaaa
     6841 aatcgaaaga atatgcagtt ttattagaat ggaataacat ttattatgat gaaagtccac
     6901 gatttattaa tactgaaaga agtattattc gccttgggtt ggtgcaatgg caaatgcgtt
     6961 cgctgagtaa cttggaagag tttttcaacc aagccgagtt ttttatcgat gctgtttcgg
     7021 gttatgaaag cgattttgca ttgtttcccg agttgtttat tgcaccgttg atggcagatt
     7081 ataatcacct ttcagaggca gatgccattc gagaacttgc caaatatgcc gaacctatta
     7141 aaaagaaatt tcaagaattt gccatatctt ataatatcaa tatcatcagt ggcagtatgc
     7201 cttatctgga agacggcatt ctatacaacg ctggttttct ttgcaaaaga gatggcacaa
     7261 atgaagtgta caaaaaaatt cacatcacac ccaacgaagt gttccattgg ggaattactg
     7321 gtggcagcaa aattcaaacc tttgacaccg attgtggaaa aattggcgtt gttatttgct
     7381 atgatattga gtttccagaa ttatctcgac taatggcaga cgaaggaatg aacattcttt
     7441 ttgtaccgtt tttaaccgat actcaaaacg gttatactcg tgtaaaatgt tgtgctcaag
     7501 ccagagccat agaaaacgag tgttatgttg ccattgctgg ctgtgttgga aatcttccaa
     7561 aagtgaacaa catggacatt caatatgcgc aagcggctgt ttttacacca tcagattttg
     7621 cttttcctag caacggaatt aaagccgaag ccacaccaaa tactgagatg acactgattg
     7681 ttgatgtaga tattaacctt ttaaaagaac ttcacgaaca tggtagtgtt aaaataatga
     7741 aagatcgaag acatgatttg tatagtataa aaaaattgaa gtaagttggg tttaaaaaat
     7801 ggattagtct taaaattaaa caatcgatta tgacaaagaa ctgtttagaa tgtaatgaaa
     7861 aaattattgg tcgcgaggac aaaaagtttt gcagcgatgg ttgtcgtaat agttacaaca
     7921 acaaaataaa caaagacagc accaattata tgcgaaacat caacaacaag ttgcggaaaa
     7981 attatcgcat tttgagtgct ttaaacattg atgggaaatc taaaaccact aagtcaaaac
     8041 tgctgagcaa aggatttgac tttgaataca tgactactat tttgcacacc aaaactggaa
     8101 atacctatca ttttgtgtac gatcaaggat atatggtttt agacaatgac ttttatatgt
     8161 tagttaagaa agatatttaa taaagttcgc atgaaaaaca accaaccgac cataattgtt
     8221 gccttacttt tgattggatt actttcgggt attttctact ttatgatgcc acaaagttat
     8281 gacacttcag aagcaccact ttctgagttt tcaaccaaac gagctttaga aaaagtgaag
     8341 cacatttcgc aaaaaccaca ttatgttggt tcgcaaaatc atgttttagt ggcaaactac
     8401 ataaaaaaag agttgcaaga tttaggcttg caaacttccg atcaagaagg ttttgtaatg
     8461 accgaaaaag gaacgttggt aaaagccaaa aacattttag ccaaaataaa aggcacttct
     8521 tctgcgctca gtgagactaa taattctaaa tcattggtat tactttctca ctatgattct
     8581 gcaccacatt cctattcact aggtgctagt gatgatgctt ctggtgtagc aacaattata
     8641 gagagtgttc gaggattttt gcacaataaa accaaacata aaaacgacat cattattctt
     8701 tttactgatg ccgaagaaat aggtttgaat ggtgcagcac tttttacaac acaacatcag
     8761 tgggcaaaag aggttggctt ggtattaaac tttgaggcac gtggctcgtc tggtccgagt
     8821 tatatgttga tggaaaccaa caaaggaaat agcaagatgg ttgatgcttt taaggccgga
     8881 aaagcacctt ggtctgtttc caattcattg atgtatagca tttataaaat gttgcccaac
     8941 gatactgatt tgacagtttt tagagaagta aaacaaatac aaggatttaa ttttgcgttc
     9001 attgatagtc attataatta tcatacatct cgcgataatt atcaaaacct ttcgagagaa
     9061 accttagcac atcaaggaag ttatttgttt ccgctactaa cttatttttc aaacgctgat
     9121 ttaagtaatt tgaatgctac taacgaccaa gtatatttca acattccttt ttcgtttgtg
     9181 agttatccct tttcatggat tcttcccatg ttactcatca cttttggatt attcttattg
     9241 tttgtgtttg ttggtttagg aaaaagagct ttattattag acgaagtttt caaaggtttt
     9301 aaaccgtttt ttggtgcgtt aattacgtct ggtgcagtta cttatattgg ttggaaatta
     9361 cttttgaatt tttatcctca atacaacgac atcctccacg ggtttactta taatggtcac
     9421 gattatattt atgcttttgt gagtttgact ttaggtattt gttttttatt ttataaaaaa
     9481 caagccaagc acaacactgc aatgaatcaa ttatttgctc cattattaat ttggttacta
     9541 ctaaatactg ccattgcttt taaacttgaa ggtgcaggat ttttaatcat tcctgtaatt
     9601 tgtagcacta taatgttggc agtttatgtg tttacccaaa gaagttattg ggttttaaat
     9661 ttgattttgt ctattccagc attactgctt ttagtaccat ttattatgat gtttcctatt
     9721 gggttagggt taaaaatact ttttggaagt gctgttttaa ccgtacttgc ctttacgctt
     9781 ttacttccga tttttggaaa ttttgaaaag aagaatttat ggtcaatttt attcttttta
     9841 ttatccattg gattattcat aaaagcacat caaatctcag agtttagtac tgaacgacca
     9901 aagccaaaca gtttagtata tctattgaat gcagatactc aaaaagccta ttgggctact
     9961 tatgacaaaa atttagatga atggaccaaa ggatatttgg gcgaaaaacc agctgatgca
    10021 aaaatattaa atactaataa actgtacagc aaatatggtt cttcctatac ttatatggct
    10081 gaagctccga taaaaaatat ttcttcgcca aacattgagt ttttaaccga ttctatcatt
    10141 ggaagtcaac atgtttataa aataaaaatt tcacccaatc gaaatgtaaa tcgatatgat
    10201 gtatttgtaa aaaacggaat tactattaat agtttaatcg ctaatggcgt aaaatcaatt
    10261 gattttaaaa gcaatattgc aagtaaaact tcgggtaaaa ttttaagtta ttatgtagtt
    10321 gaaaatattc cgttagaatt agagttttcg ataaaagcta atgataaatt agatttagaa
    10381 ttattagaga gttcttttga tttgatgtca aatactgatt ttacaatcac caaaagaaag
    10441 tcgtggatga ttccaacacc ttttgtatta accgatgctg tgattattag acaaaaagta
    10501 aaaccatcaa ctcatagcag tctaagaaca ccaagaattg ctttgcctag ccgaagatat
    10561 acttcgcccg atagtttgaa tgttgtaatg gatagtttaa aataatgaca caaatatcaa
    10621 tacttggttg cggttggttg ggtttacctt tagcaaaatc tttgatagaa aaaggttttt
    10681 gggttaatgg ttctactaca tctttagaga aaattgctgt tttaaaaaac gaaggaataa
    10741 ctgcttttca aattgaagta acagaaaacg aaatcattgg aaaccttgaa aattttctta
    10801 aaaattcagc aattttaatc cttgatattc cacccaaatt acgtggaaat caacctgaaa
    10861 attttgttaa aaaaatcaaa aacatacttt catttgttga aaattcgagt gttcaaaaag
    10921 tgctttttat tagttcaact tcggtttatg cggatgataa ttctaaaatt aatgaagaaa
    10981 caaaacctca acccgaaaca gaaagcggaa aacaactttt agaagtagaa aatcttttaa
    11041 aagcgaattc aaattttcaa actacaataa ttcgttttgg tggattgatt ggtgatgata
    11101 gaaatccaat acatttttta gcaggaagaa aaaatattca aaatcctgat gcgccaatta
    11161 atataataca tctaaaggat tgtattggag ttattgaatc tgttattgaa aaaaatattt
    11221 ggaacgaaac ttttaatgct gtttgtccat ttcatcctac tagaaaagac tattatactc
    11281 aaaaagctat tgcgctaaat ttaccaattc ccgaatttga tttttcaaat aaatccattg
    11341 gaaaaatcat ttcttcaaca aaaacagaaa ccgttttaaa ctatactttt caaaaaacta
    11401 atttatgaat tttaatgatt tgattggaac tattggcgtt ggaattattc ttcttgctta
    11461 ttttttgaat attttttcct tgataaaaaa agacggaatt ctattttatt tgatgaatat
    11521 tattggtgca agcattgcgt gctttgcatc ggttttaata agctatatgc cattcataat
    11581 ccttgaagca acttgggcaa tcgttagtgt gattggatta cttaaagtca taaaaaatgc
    11641 cccaaatagt gattaacttt ttgaggcatg tttagtataa tttgaagttt tttatttttt
    11701 gtttaccaaa tcaaaactct tttttctggt gctacataca ttccgtcgcc aggcttaata
    11761 ccaaaagctt ggtggaatgt ttccaaattt aataatggca catacccacg atacattcct
    11821 ggagaatgcg gatcagtttt tacttgattt ttgatggctt catcgcgcat tttacttc
//
LOCUS       DAOOBH010000069        11609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_7082_length_11609_cov_7.574520, whole genome shotgun sequence.
ACCESSION   DAOOBH010000069 DAOOBH010000000
VERSION     DAOOBH010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7082_length_11609_cov_7.574520"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /locus_tag="PK218_07845"
     CDS             complement(<1..1677)
                     /locus_tag="PK218_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPW98457.1"
                     /translation="MKFLKLLILFLFIQTQAQQGGMWIPSLLKGMNETEMKNLGMKIS
                     AEDIYSVNKSSLKDAVPHFDGGCTAEMISNKGLLLTNHHCGYDNIQSHSTVENDYLTN
                     GFWAYSMAEELPNKDLYVTFINRIEDVTTKVLEGVGTLTNESDKQKKIQENISNLNKS
                     LPKESWQENSIRTFYDGNQYILFVTETYRDVRLVGAPPSSIGKFGSDTDNWVWPRHTG
                     DFSLFRVYADKNNKPAEYSKDNVPYVPKHFFPISLENLKENDFTMVMGYPGRTQEYLP
                     SFAVEQIVNTLNPAKIEVRDAALKVQDGFMRKDNAIKIQYASKYASVANYWKKWIGET
                     KGLKKSNAIGVKQKFEKEFAEKVAKAGKQAEYGNLLNEFEKNYKEINDYALARDLFSE
                     VALRNTELLSVGYKLYQLEQILNTKGEQSFNDRRNNLINGLGDFYKDFNAKVDEKVFE
                     QLIAIYSNRYPSQFLSVDLKNIDADKLSKDVYANSKLVSYDKIKELLTGDSKTVIANF
                     NTDKGFQVVKAIADSYFQNIAPKYDEINLKNTALQRTYMKAILELSPKSAR"
     gene            complement(1729..2973)
                     /locus_tag="PK218_07850"
     CDS             complement(1729..2973)
                     /locus_tag="PK218_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPW98458.1"
                     /translation="MLFIYNLIVLIASQFLKILAVFSPKIKLFVDGRRNVFEQLENRI
                     AKTDKSIWFHAASLGEFEQGLPVMEKIKEKFPSHKIVVTFFSPSGFEVRKNNTIADVT
                     VYLPLDTKSNAKRFLKIVNPDLVFFIKYEYWPNYLNELKHSSSKTYLISGIFREKQVF
                     FRWYGGFYRNALKSFDYFFVQNESSIKLLQKLGITNVKISGDTRFDRVVSILERDNSL
                     NFIEQFKNDTTTIVIGSSWSKDENYLVNYINNFNGNTKFIFAPHNIKTEQIQELKKSI
                     SKKTILFSEKEHQNLASFQVMIVDTIGILTKIYSYADIAYVGGGFGTAGLHNVLEPAT
                     FGIPIVIGPNFSNFAEAIALVKLEGCISVTNQIELNEALDILGQNEAERLEKGHICAT
                     FVQMNKGATKIILNHVLNETLN"
     gene            3070..4206
                     /locus_tag="PK218_07855"
     CDS             3070..4206
                     /locus_tag="PK218_07855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPW98459.1"
                     /translation="MRKLQMVDLKSQYDKIKPAVDASIQEVLETTTYINGPKVHEFQK
                     QLEEYLGVKHVIPCANGTDALQIAMMGLGLQPGDEVITADFTFAATVEVIALLQLTPV
                     LVDVDLHNMNISLEAIKKAITPKTKAIVPVHLFGRAANMEAIMTIAKEHNLYVIEDNA
                     QAIGANCVFSDGTKKKAGTIGHVGATSFFPSKNLGCYGDGGAIFTNDDDLAHTIRGIV
                     NHGMYVRYHHDVVGVNSRLDSIQAAVLNIKLALLDDYNLARQHAARKYSAAFEGNNNI
                     VAPMICDDCDCHVFHQYTLRITNGKRDQLLDHLQAKQIPCAIYYPIPLHSQKAYLDPR
                     YKEEDFPTTNQLVQEVISLPMHTELDDEQIKFITESVLEFFNLE"
     gene            4215..5231
                     /gene="galE"
                     /locus_tag="PK218_07860"
     CDS             4215..5231
                     /gene="galE"
                     /locus_tag="PK218_07860"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPW98460.1"
                     /translation="MKKVLVTGGLGFIGSHTVVELHNAGFEAIIIDNLSNSSINVLDG
                     IERIIGYKPNFEELDLRDKASVKSFFGKHTDVAGVIHFAASKAVGESVENPLLYYENN
                     INTLVYILQELQQKEEANFIFSSSCTVYGQAEKMPITENASVQPAMSPYGNTKQIGEE
                     IITDVTKVTNINAILLRYFNPVGAHPSAEIGELPIGVPQNLIPYITQTGIGLRQELSV
                     YGDDYPTPDGTAIRDYIHVVDLAKAHVVAMERLINKKNLSKVETFNLGTGTGSSVLEV
                     ITAFEKVSDKKLPYKIVGRREGDITSAYANTDKANEILGWKASLTLEESLASAWKWEQ
                     KIRS"
     gene            complement(5236..5484)
                     /locus_tag="PK218_07865"
     CDS             complement(5236..5484)
                     /locus_tag="PK218_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98461.1"
                     /translation="MKKIFLSLAVFATLTVVSCKESTTDKVEDATEAVGNDIENSVED
                     AGNTIDSTATEIGNDVKDATKKGAEKVEEKAKEVKESM"
     gene            complement(5495..6196)
                     /locus_tag="PK218_07870"
     CDS             complement(5495..6196)
                     /locus_tag="PK218_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPW98462.1"
                     /translation="MKKKLEILSKVKASKVISPEINFEDYIAIDFSESNIDLQNHSLE
                     NAVDYEIYIQKHLDKNNAKIAFGGYNEVRNLYKRSTVFKNNETEERNVHIGLDLWINK
                     QATIHAALDGEIHSFQNNTSLGDYGPTLILKHEVDGFVFHTLYGHLSEESLEKKMGKK
                     ITSGEKIGALGLPPINGDYAPHLHFQIIIDMENKMGDYPGVSSKINLEYYNQNCPNPE
                     LLLKIREKFNNFENL"
     gene            complement(6206..7078)
                     /gene="fabD"
                     /locus_tag="PK218_07875"
     CDS             complement(6206..7078)
                     /gene="fabD"
                     /locus_tag="PK218_07875"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPW98463.1"
                     /translation="MKAYIFPGQGAQFTGMGKGLYENSSLAKELFEKANKILGFRITD
                     IMFEGTAEELKETKVTQPAVFLHSVILAKTLENFKPDMVAGHSLGEFSALVANGALSF
                     EDGLKLVSQRALAMQKACELKSSTMAAVLNLDDKIVEDICASIDGIVVAANYNCPGQL
                     VISGEYSAVELACEKMKEAGAKRALILPVGGAFHSPMMEPAREELAAAIEATTFSTPI
                     CPVYQNVTASAVSSAEEIKKNLITQLTAPVKWTQSVNQMIADGATSFTEVGPGKVLVG
                     LVNKINKEIETISA"
     gene            7203..8831
                     /locus_tag="PK218_07880"
     CDS             7203..8831
                     /locus_tag="PK218_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98464.1"
                     /translation="MKKVFASICLVALAFLLIYLFLPVLRYGFGGFALILLLLLIIGM
                     ILSLGIKSDSKSKKIVLQSKPNKLFGIGILLLLIYLIVIPIITSATIFHTSSYQKLIG
                     EVKNGVKIGNHFEPISIDKLRVVDQKLAYLLGEKIIGSQPALGSQIDLGEFSIQKVNN
                     ELYWVAPLLHSGFFKWLNNSEGTPGYVMVSATNERDVRLVQTINNQPIKIKYQPNAYF
                     QSDIHRHVYFNGYATIGLTDFTFEIDESGKPFWVITTYDKKVGFSGDDATGILVVDAQ
                     TGEIKQYSISVAPTWVDRIQPTDLIETQLNDWGEYINGYWNFSNVNKLQITEGMSLVY
                     GKDNKSYWYTGLTSVGNDESSVGFVLVDTRTKETTFYKQSGATEYAAQSSAEGKVQEK
                     GFRASLPIPYNINNIPTYVMTLKDDGGLVKMYAMVAISDYTIVGVGNSMRETLTAFKS
                     VYNSAGNKINPSSSDAKKVLKSVITRIQSDVKNGNSFYYFTVKDYPNIFVGSSQVSNE
                     LPVTIVGDSVKISFDIDTEEVIDVTSFKNVNLKK"
     gene            complement(8868..9389)
                     /locus_tag="PK218_07885"
     CDS             complement(8868..9389)
                     /locus_tag="PK218_07885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPW98465.1"
                     /translation="MRKIIAFAGSSSKKSINKQLVTYATSLFNDAKVEILDLNDYEMP
                     LFSVDKEKENGFPDLAVQFLSKIGTADLLVVSLAEHNGAYSTAFKNILDWTSRINPKT
                     FQSKKMLLMATSPGARGGSSVLEIAKDRFPRHDAEIVGVFSLPSFNDNFDKEKGITNP
                     ELKDKLVEIINTI"
     gene            complement(9389..9613)
                     /locus_tag="PK218_07890"
     CDS             complement(9389..9613)
                     /locus_tag="PK218_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HPW98466.1"
                     /translation="MEPKDITKEYTNGDVTIVWQSAKCTHSGNCVRNNPEVFKPKEKP
                     WVTPENSETEKIIETVKKCPSGALTYYKNN"
     gene            complement(9631..9909)
                     /locus_tag="PK218_07895"
     CDS             complement(9631..9909)
                     /locus_tag="PK218_07895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW98467.1"
                     /translation="MEVKLNINEKNGIFYIEVDGKTEAKMTFVFAGDEKIIIDHTEVN
                     PGNNGKGYGKMMVQKAVEFALEKNIKIIPLCPFAKSVFDKTPDFRTVL"
     gene            complement(9912..10628)
                     /locus_tag="PK218_07900"
     CDS             complement(9912..10628)
                     /locus_tag="PK218_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPW98468.1"
                     /translation="MKNTVLHKANTRGNANHGWLNAYHSFSFASWYNPERVQFGVLRV
                     LNDDTIAAGMGFGTHPHDNMEIITIPLEGDLAHKDSMGNAATIKTGDVQVMSAGTGIQ
                     HSEFNPNADQQTKLFQIWLFPKTRNVEPRYQQITLDKSLEKNDFAQILSPNADDAGVW
                     IHQDAWFYLSDFDKDFSKKLPLKKEGNGFYIMNIEGEIEVNGEKLEKRDAIGIWETAE
                     LEIKANTTAKFLIMEIPMEN"
     gene            10866..>11609
                     /locus_tag="PK218_07905"
     CDS             10866..>11609
                     /locus_tag="PK218_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HPW98469.1"
                     /translation="MIKFYKVLFIMFFINHNSFSQIDYNKQIIKKSISQQWELDSIDK
                     KGTFRLVSYHPIYFTAARWSSRKNEQPFDESGEFATKDKNFFDNIEAKFQISFKSKLI
                     QGLLFGKVDLWVGYTQKAHWQVYNKKLSRAFRELNYEPEIILNFPLKFDLLGGQVRSF
                     GLIFNHQSNGKDIPTSRSWNRIIFNIGYEYKNWNVNFRPWYRLSDSEDENPGITKFIG
                     DGELEIGTNFGKHEFYTIISHSFSTLQKGN"
BASE COUNT         3709 a         1908 c         1840 g         4152 t
ORIGIN      
        1 tcgagctgat tttggactta attctaaaat cgctttcatg tatgtgcgct gtaatgctgt
       61 attctttaag ttaatttcat catattttgg tgcaatattt tgaaaataac tatctgcaat
      121 agcttttaca acttgaaaac ctttgtcggt attgaaattt gcgattactg ttttgctgtc
      181 accagttaat aattctttta ttttatcgta gcttaccagt ttagagttgg cataaacgtc
      241 ttttgacaat ttatctgcat ctatattttt taaatcaacc gataaaaatt gacttggata
      301 acgatttgaa tatattgcaa ttaattgttc aaaaaccttt tcatctactt tggcattgaa
      361 atctttgtag aaatcgccca aaccatttat taaattgttt cttctatcat taaaagattg
      421 ttccccttta gtgtttagga tttgttctaa ttggtatagt ttataaccca ctgatagcaa
      481 ttcggtatta cgcaaagcta cttcgctaaa caaatctctt gccaatgcat aatcattaat
      541 ttctttataa tttttttcaa attcgttgag taaatttcca tattcagctt gctttcctgc
      601 tttggcaact ttttctgcaa attctttttc aaacttttgt tttacaccaa ttgcatttga
      661 tttttttaaa ccttttgttt caccaatcca ttttttccaa taattggcaa cacttgcata
      721 tttggatgca tattgaattt tgatagcatt atcttttctc ataaaaccat cttgtacttt
      781 taatgcagca tcacgaactt caattttggc gggatttaaa gtatttacaa tttgttctac
      841 agcaaatgat ggtaaatatt cttgtgttct gccagggtaa cccatcacca tcgtgaaatc
      901 attttctttt aaattttcta aagaaattgg aaagaaatgt tttggcacat aaggaacatt
      961 gtctttagaa tattctgctg gtttgttgtt tttatctgca taaactctaa acaatgaaaa
     1021 atctccagta tgtcttggcc aaacccagtt atctgtatcg gaaccaaatt ttccaattga
     1081 actaggtggc gcaccaacta atcgaacatc tctatatgtt tcggttacaa acaaaatata
     1141 ttgatttccg tcataaaaag ttctaatgct attttcttgc caactttctt ttggtaacga
     1201 tttattcaaa ttagaaatat tctcttgaat tttcttttgt ttgtcacttt cgtttgtcaa
     1261 agttccaaca ccttctaaaa cttttgtagt tacatcttct attctgttaa taaaagttac
     1321 atacaagtct ttgtttggca attcttccgc catgctgtat gcccaaaaac catttgttaa
     1381 ataatcattt tcaaccgtag aatgactttg aatattgtcg tatccacaat gatgattcgt
     1441 caacaaaagt cctttatttg aaatcatttc agcagtacaa ccaccatcaa aatgtggcac
     1501 agcgtctttt aaacttgatt tgtttacaga ataaatatct tctgctgaga ttttcattcc
     1561 aaggtttttc atctcggttt cattcattcc ttttaacaag gacggaatcc acatacctcc
     1621 ttgttgtgct tgggtttgaa taaaaaggaa taaaataagt agttttaaaa atttcatttt
     1681 ttttaatttt attgaggtgc aagatacaat ttttgctttt ttagaaaatc aatttaaggt
     1741 ttcattaaga acatgattta aaataatttt agttgctcct ttattcattt gtacaaaagt
     1801 ggcgcaaata tgtccttttt caaggcgttc tgcttcattt tgaccaagta tatctaaggc
     1861 ttcgttcaat tcaatttgat tggttaccga aatgcaacct tctaatttta ccaaagcaat
     1921 ggcttcggca aaattggaaa aatttggacc gatgacaatt ggaatcccaa aagtggcagg
     1981 ttccaaaaca ttatgtaaac ctgcggttcc aaatccgcct ccaacataag caatatctgc
     2041 atacgaataa attttggtca aaattccgat ggtatctaca atcatcactt gaaaactcgc
     2101 aagattttga tgttcttttt ctgaaaacag aatagttttt tttgaaattg atttttttaa
     2161 ttcttgaatt tgttccgttt taatgttgtg tggagcaaat ataaattttg tatttccatt
     2221 aaaattattg atgtagttga ctaaataatt ttcatctttt gaccaagaac ttccaataac
     2281 aattgttgtt gtgtcatttt taaattgttc aatgaaattt aatgaattat cgcgttctag
     2341 tattgaaact acgcggtcaa aacgtgtgtc tcctgaaatt tttacattcg tgatacctaa
     2401 tttttgaagt aattttattg aactttcgtt ttgcacaaaa aaataatcaa aacttttcaa
     2461 agcatttcta taaaatccgc cataccatct gaaaaacact tgcttttccc taaaaattcc
     2521 tgaaatcaaa taagttttac tactcgaatg ttttaattca ttaagataat ttggccaata
     2581 ttcatattta ataaagaaaa ctaaatcagg atttacaatt ttcaaaaatc gtttggcatt
     2641 tgatttggta tccaatggta aatacactgt aacatctgca atggtattat tttttcgaac
     2701 ttcaaaacct gatggcgaaa aaaaagtaac tacaatttta tgacttggaa acttttcttt
     2761 gattttttcc atcactggca aaccttgttc aaattcaccc aaagaagcgg catgaaacca
     2821 aatggattta tctgttttgg cgattctatt ttctaattgt tcaaaaacat tcctcctacc
     2881 atcaacaaac aattttattt taggactaaa aaccgctaaa atcttgagaa attgcgaagc
     2941 gattaaaaca attaaattat agataaacag catgatcttt gaattgtgtc gctaaaatac
     3001 atcagtttat agaaatttca ttgcaatacg tcattaaatt agtagttttg ctttataaat
     3061 ttttgcccaa tgagaaaact ccagatggtt gaccttaaaa gtcaatatga taaaataaaa
     3121 cctgccgttg atgcttcaat tcaagaagtt ttagaaacaa caacctatat aaacggtcca
     3181 aaagttcatg aatttcaaaa acaacttgaa gaatatctag gtgtaaaaca cgttattcct
     3241 tgtgcaaacg gaaccgatgc tttgcaaatt gctatgatgg gattaggttt acagcctggt
     3301 gatgaagtga ttaccgccga ttttacattt gctgcaactg ttgaagtgat tgcgcttttg
     3361 caattaacac cagttttggt tgatgttgat ttgcacaaca tgaatatttc tttagaggca
     3421 attaaaaaag caataacacc aaaaaccaaa gcgatagttc ctgtgcatct ttttggtcgt
     3481 gctgccaata tggaagcgat tatgactatt gctaaagaac ataatttata tgtaattgag
     3541 gataacgcac aagcaattgg agccaattgt gtcttttcag acggaacgaa aaagaaagct
     3601 ggaacaattg gtcatgttgg agcaacttcc ttttttccga gtaaaaatct tgggtgttat
     3661 ggtgatggtg gtgcaatttt taccaacgac gatgatttag cacataccat tcgaggaatc
     3721 gtaaatcacg gaatgtatgt tcgttatcac catgatgtag ttggcgtaaa ttcaagatta
     3781 gacagtattc aagccgcagt tttaaatata aaattagcac tgcttgacga ctataattta
     3841 gcacgtcaac atgcggctcg aaaatattct gccgcttttg aaggaaataa taacatcgtt
     3901 gctcctatga tttgcgacga ttgcgattgt catgtttttc atcaatacac attgcgaatt
     3961 acaaatggaa aaagagatca attactagac catttgcagg caaaacaaat tccctgtgcg
     4021 atttattatc caatcccgct gcacagtcaa aaagcttatt tagatcctcg atacaaagaa
     4081 gaagattttc caactaccaa tcaattggta caagaagtta tctcattgcc aatgcatacg
     4141 gaattagacg atgaacaaat caaatttatc acagagtctg ttttagaatt ttttaattta
     4201 gaataatatt taacatgaaa aaagtattag taactggcgg attaggtttt ataggttcgc
     4261 acaccgtagt tgaattacat aatgcaggtt ttgaggctat tattattgat aatttatcca
     4321 attcttctat caatgttttg gatggaatag agcgcattat tggttacaaa cctaattttg
     4381 aagaattaga tttaagagac aaagcatcag ttaaaagttt ctttggaaaa catactgatg
     4441 tcgcaggtgt gatacatttc gcggcatcaa aagcagttgg tgaaagtgta gaaaatccat
     4501 tgttatatta cgaaaacaac atcaatactt tggtttacat tctgcaagaa ctgcaacaaa
     4561 aagaagaagc taattttatt tttagttctt cctgcacagt ttatggacaa gctgaaaaaa
     4621 tgcctattac tgaaaatgct tctgtgcaac ccgccatgtc accatatgga aacactaaac
     4681 aaattgggga agaaatcatt acagacgtaa ctaaagtaac caatataaac gctattcttt
     4741 tgcgctattt taatccagtt ggtgctcatc caagtgcaga aattggggaa ttgccaattg
     4801 gtgttccaca aaatttaatt ccctatatta cacaaacagg tattggattg cggcaagaac
     4861 tttcggttta tggtgatgat tatccaacac cagatggaac tgcaattcgc gattacattc
     4921 atgttgtcga tttggcaaaa gcacatgttg tagcaatgga aagactcatc aataagaaga
     4981 atttgtcgaa agtagagact ttcaatctag gaaccggaac tggaagttct gttttagagg
     5041 tgattactgc atttgagaag gtttcggata aaaagcttcc ttacaaaatt gttggaagaa
     5101 gagaaggtga tattacttct gcctatgcca acacagataa agccaatgaa attttaggct
     5161 ggaaagcaag tctaacatta gaggaaagct tagcttcagc ttggaaatgg gaacagaaaa
     5221 ttagaagtta aaaaattaca ttgattcttt tacttctttt gctttttctt ctactttttc
     5281 ggcacctttt tttgttgcat cttttacatc gtttccaatt tcagttgcag tcgaatctat
     5341 tgtgtttcca gcatcttcta cgctgttttc aatatcattt ccaacagctt ctgtagcgtc
     5401 ttcaaccttg tcagtggttg attctttaca cgacacaact gttaaagttg cgaaaacagc
     5461 caagcttaaa aaaatttttt tcataataac taatttatag attctcaaag ttattgaatt
     5521 tttcgcggat tttcaacagt aattctggat ttggacagtt ttggttgtaa tattctaaat
     5581 tgattttact cgaaacacct ggataatcac ccattttatt ttccatatca attataattt
     5641 ggaagtgcag atgtggtgca taatctccat taattggcgg caatcctaat gctcctattt
     5701 tttcgcccga tgttattttt ttgcccattt ttttttccaa actttcttcg ctcaaatgtc
     5761 cgtaaagcgt gtgaaaaaca aaaccatcca cttcatgttt taaaattaga gttggaccat
     5821 aatctcctag ggaagtgttg ttttgaaaac tgtgaatttc tccatctaat gccgcatgaa
     5881 tggtagcttg tttattaatc cataaatcca aaccgatgtg aacgtttcgc tcttcagttt
     5941 cgttgttttt aaaaacagtg cttcttttat acaaatttcg aacttcgtta taaccgccaa
     6001 aagctatttt agcattgttt ttatctaaat gtttttgaat ataaatctcg taatctactg
     6061 cattttctaa agaatgattt tgtaaatcaa tatttgactc tgagaaatca atcgcaatat
     6121 aatcttcaaa attaatttcg ggtgatatga cttttgaagc ttttactttt gaaagtattt
     6181 ctaatttttt tttcattttt gagtattatg cagaaatcgt ttctatttct ttattgattt
     6241 tatttaccaa tccaaccaat acttttcctg gaccaacttc tgtaaaactt gttgctccgt
     6301 ctgcaatcat ttggtttaca gattgcgtcc attttactgg agccgttaat tgtgtaatta
     6361 aatttttctt gatttcttca gctgaagata cagcacttgc cgttacattt tgataaaccg
     6421 gacaaattgg agttgaaaac gttgttgctt caattgccgc tgccaattct tctctagctg
     6481 gctccatcat tggtgagtga aaagctcctc caactggcaa aatcaatgct cgttttgcac
     6541 cagcttcttt cattttttca caagctaatt caaccgccga gtattcccca gaaattacta
     6601 attgtcctgg acagttataa tttgcagcta ctactatacc atcaatcgaa gcacaaatat
     6661 cttcaactat tttatcatct agatttagaa cggcagccat tgttgaagat tttaattcac
     6721 aagctttttg cattgccaaa gctcgttgtg agactaattt taatccatcc tcaaatgaca
     6781 aagcgccatt agcaaccaaa gcggaaaatt caccaagtga atgaccagca accatatcgg
     6841 gtttaaaatt ttctaatgtt ttagccaaaa ttacagaatg caaaaaaaca gcgggttgcg
     6901 ttacttttgt ttctttcaat tcttctgctg ttccttcaaa catgatgtcg gttattctaa
     6961 atccaagaat tttgtttgct ttttcaaata attcttttgc taaacttgag ttttcgtata
     7021 aacctttacc cattcctgta aattgagcac cttgtcccgg aaaaatatat gctttcatta
     7081 aatttaagtt ttgttaattt acaaaaatac taatttagaa attaactaat gcttctatta
     7141 catgatttga agtgtaactt ttaaacacat tattacacat ataaaaacat aaatataaaa
     7201 ttatgaaaaa agtattcgct tcaatttgtt tggttgcatt agcattttta ttgatttatc
     7261 tatttcttcc tgtactccga tatggatttg gtgggtttgc actaatttta ttgctgttat
     7321 tgattattgg tatgatttta tctttgggaa ttaaatctga ttccaagagt aaaaaaattg
     7381 tgcttcaatc aaaacctaat aaattatttg ggattggaat tttacttctg ttaatttatt
     7441 taatcgttat accaataatt acatctgcta ctatttttca tacgtcaagc tatcaaaaat
     7501 taataggcga agttaagaat ggcgtgaaaa taggaaatca ttttgaacca atttccatag
     7561 acaaacttag agttgttgac caaaagttgg cttatctttt aggtgaaaaa attataggat
     7621 cgcaaccagc tttgggaagc caaattgatt tgggcgaatt ttctattcaa aaagtgaata
     7681 atgaattgta ttgggttgct ccattgttgc attctgggtt ttttaaatgg ctgaataaca
     7741 gtgaaggaac acccggttat gtaatggttt ctgctactaa cgaacgtgat gtaagattag
     7801 tgcaaaccat caataatcaa cctataaaaa ttaaatacca accgaatgct tattttcaaa
     7861 gtgatattca tagacatgtt tactttaatg gatatgctac tattggatta actgatttta
     7921 cttttgaaat tgatgaatca ggaaaaccct tttgggtaat tacaacttat gataaaaaag
     7981 ttggtttttc tggtgatgat gccacaggga ttttagttgt tgatgcgcaa acaggagaaa
     8041 taaaacagta ttcaatatca gttgcaccaa cttgggttga tagaattcaa ccaacggatt
     8101 taatagaaac acaattaaac gattggggcg aatacatcaa cgggtattgg aatttttcca
     8161 acgttaataa attgcagatt accgaaggaa tgagtttagt ttatggaaaa gacaataaat
     8221 cttattggta cactggtttg acttctgttg gaaatgatga gtcttctgtt ggttttgttt
     8281 tggtagatac tagaactaaa gaaacaactt tctacaaaca aagtggtgct acagaatatg
     8341 cagcacaaag ttcggcagaa gggaaagttc aagaaaaagg atttcgtgcg tcgttaccaa
     8401 ttccatataa catcaataat atcccaacct atgtaatgac tttgaaagac gatggaggct
     8461 tagtaaaaat gtatgcgatg gttgctatta gcgactatac gattgttggt gtcggaaatt
     8521 cgatgcgaga aaccttgacg gcattcaaaa gtgtttacaa ttcggcggga aataaaataa
     8581 atccttcaag ttcagacgct aaaaaggtat tgaaatctgt tattactcgt attcaaagcg
     8641 atgtcaaaaa cggaaatagt ttttattatt tcaccgtgaa ggattaccct aatatttttg
     8701 tgggttcatc tcaggtttct aatgaattgc cagtaaccat tgtaggcgat agtgtgaaaa
     8761 tttcttttga tatagatacc gaagaagtaa ttgatgtgac gagttttaag aatgttaatt
     8821 tgaagaaata atattaaaaa atccagcttt attgctggat ttttttctta aatagtattt
     8881 ataatttcaa ctaatttatc ctttaattct ggattcgtaa ttcctttttc tttgtcaaag
     8941 ttatcattaa agcttggtaa cgaaaacaca ccaactattt cagcatcatg tctcggaaaa
     9001 cggtctttgg ctatttctaa cacggaactt ccaccacgag caccaggaga agttgccatc
     9061 aaaagcattt ttttgctttg aaatgttttt gggtttattc tcgatgtcca atcaaggata
     9121 tttttgaaag ccgtcgaata ggctccatta tgttcggcca gtgaaacaac aagcaaatct
     9181 gcagtaccaa tttttgaaag aaattgaact gctaaatcag gaaagccatt ttctttttct
     9241 ttgtcaaccg aaaacaaagg catttcataa tcatttaagt ccaagatttc tactttagca
     9301 tcattaaata aagatgttgc ataagtaacc agttgtttgt tgatggattt tttgctagaa
     9361 gaaccagcaa aggcaataat tttcctcatt agttgttttt ataataggtt agtgctccag
     9421 atggacattt ttttacagtt tctataattt tttcagtttc cgaattttca ggagttaccc
     9481 aaggtttttc ttttggttta aatacttcag gattatttcg aacacaattt ccagaatgtg
     9541 tgcattttgc cgattgccaa acaatggtta catcgccatt tgtgtattct tttgtaatgt
     9601 cttttggttc catgatattt gtgtttaaat ttataaaaca gttctaaaat cgggtgtttt
     9661 gtcaaaaacg ctttttgcaa aaggacaaag aggaataatt ttgatgtttt tttctagtgc
     9721 aaattctact gctttttgaa ccatcatttt tccataccct tttccgttat ttcctgggtt
     9781 tacttcggtg tgatcaataa ttattttttc atcgccagca aacacaaaag tcattttggc
     9841 ttcggttttt ccatcaactt caatgtagaa aattccgttt ttttcgttta tatttaattt
     9901 aacttccata attaattttc cattggaatt tccattatta aaaatttggc agttgtattt
     9961 gctttgattt ctagctcagc tgtttcccaa attcctattg catccctttt ttctagtttt
    10021 tcgccattta cttcaatttc accttcaata ttcatgatgt aaaacccgtt tccttctttt
    10081 tttagaggaa gttttttaga aaaatctttg tcaaaatcag acaaataaaa ccatgcgtct
    10141 tgatgtatcc atacgccagc atcatctgcg tttggagata aaatttgagc aaaatcattt
    10201 ttttctaatg atttgtctaa agtaatttgt tggtatctag gctctacatt tctcgttttt
    10261 ggaaatagcc agatttgaaa taacttcgtt tgttggtcag catttggatt aaattctgaa
    10321 tgttgaattc cagttccagc actcatcact tgcacatcgc ctgttttaat agttgcagca
    10381 tttcccatac tgtctttatg agctaaatca ccttctaatg gaattgtgat aatttccata
    10441 ttatcgtgtg gatgcgtccc aaaacccatt ccagctgcaa ttgtgtcgtc atttaaaacg
    10501 cgcaaaacac caaattgtac tctttctgga ttataccaac tggcaaaact gaaactgtgg
    10561 taagcattca gccaaccatg atttgcattt cctcttgtat ttgctttgtg taaaacggta
    10621 tttttcataa tgtataaatt tactaattat tatgatacaa atttacaacc gcttcaaatg
    10681 aaaagcactt aacctagatt aagaaataaa aaaactctta atagcattga tttttacaaa
    10741 cattaaaaat agatttcatc atcataaaat agtataatta tataaatgaa aacaaaatta
    10801 atgcaacaag ttgtgtcaat gtttgtttta acttcgtggt tttatactaa tttacatgca
    10861 acactatgat aaaattttac aaagtacttt ttattatgtt ttttataaat cacaattctt
    10921 tctcacaaat tgattataat aagcaaatta tcaaaaaaag catatcgcaa caatgggaat
    10981 tagacagtat tgataaaaaa ggaactttcc gattagttag ctatcatcca atttatttta
    11041 ctgcagcacg ttggtctagc agaaaaaacg aacagccatt tgacgaaagt ggtgagtttg
    11101 caactaaaga taaaaatttt tttgacaata ttgaagctaa atttcaaatt agttttaaaa
    11161 gcaaattgat tcaaggatta ctttttggaa aagtagattt atgggttgga tatacccaaa
    11221 aagcgcattg gcaggtttat aacaaaaaac tttctcgggc ttttagagaa ttaaattatg
    11281 aacctgaaat aattttaaat tttcctttaa aatttgattt acttggaggt caagttagaa
    11341 gttttgggtt gatttttaat caccaatcca acggaaaaga tataccaaca tcaagaagtt
    11401 ggaatagaat tatttttaat ataggctatg agtataaaaa ttggaatgtt aattttagac
    11461 cttggtatcg tttatctgat agtgaagatg aaaaccctgg tatcacaaaa tttattggtg
    11521 acggtgaact agaaatagga accaattttg gtaaacatga gttttacacc attatatctc
    11581 attcgttttc gacacttcaa aaaggaaat
//
LOCUS       DAOOBH010000070        11518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_7162_length_11518_cov_4.732880, whole genome shotgun sequence.
ACCESSION   DAOOBH010000070 DAOOBH010000000
VERSION     DAOOBH010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7162_length_11518_cov_4.732880"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..2921
                     /locus_tag="PK218_07910"
     CDS             291..2921
                     /locus_tag="PK218_07910"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPW98470.1"
                     /translation="MSIPAQFNAKEAEQKWYNYWMENDYFSSKPDHRTPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLIRRARLKGFNACWVPGTDHASIATEAKVVAKLKSEGINK
                     SDLTREEFLKHAYDWTDKYGGTILEQLKQLGCSCDWSRTKFTMDEDMSASVIKSFVDL
                     YKKGMIYRGYRMVNWDPEAKTTLSDEEVIFEERQGKLYYIKYQIEGSEEFLTVATTRP
                     ETIFGDSAICINPNDERFTHLKGKKAIVPICGRVVPIIEDDYVDVEFGTGCLKVTPAH
                     DVNDKELGNKHNLEIIGIFNEDASLNSYGLHYQGKDRFVVRDEAAKELEALGALAKVE
                     NHINKVGTSERTKAVIEPRLSDQWFLKMDTLAKPALKAVLETEEIKLYPNRYNNTYRH
                     WLENIRDWNISRQLWWGQQIPAYFFGDGKEDFVVAETIEEALKLAQEKTSNYQLQASD
                     LRQDADALDTWFSSWLWPIAVFGGILDPENEDFKYYYPTNDLVTGPDILFFWVARMIF
                     AGYEYTGKKPFNNVYLTGLVRDKQRRKMSKSLGNSPEPLQLIEKFGADGVRVGLLLSA
                     SAGNDIMFDEELCNNGKSFANKIWNAFRLIKGWEVVEIVQPESSKVAIEWYEAKLQKT
                     LLEIEDHFEKYRLSDALMSIYKLVWDDFCSWFLEMIKPAYQQPIDKATFDKAIEMLEA
                     NLKLLHPFMPFLTEEIWQYIAERTPKQALIVAEYPVMTSFDEKLIADFEFASEVIAGI
                     RTIRKEKNISFKDTIDVMVINNENSSTHFDSIILKLGNVVVLEYISEAIEGALTFRVK
                     ANEYFIPVSGAIDVAAEIEKLTEELKYTQGFLRSVQGKLSNEKFVSGAPPQVIDNERK
                     KEADALSKIATIEQSLKSLR"
     gene            complement(2954..3451)
                     /locus_tag="PK218_07915"
     CDS             complement(2954..3451)
                     /locus_tag="PK218_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HPW98471.1"
                     /translation="MKIQIRTYIPSDLEEIVAIINYNIIYSTALYDYNTRSIEQQRII
                     FEDKLNKNFPIIVAIVDNKIVGFGYYSEFRFREAYKFTVEHSVYVSNYHHGKGIGKLL
                     LKKLIELAKAQKLHTIIGVIDSENVNSIRFHEQFGFEKVGFIKESGYKFDRWLHSVIM
                     QLLLE"
     gene            3540..5201
                     /gene="menD"
                     /locus_tag="PK218_07920"
     CDS             3540..5201
                     /gene="menD"
                     /locus_tag="PK218_07920"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HPW98472.1"
                     /translation="MIYPKIPLAQSIIEICAAKGVHDIIISPGSRNAPLTIGFTNNSK
                     FNCYSIADERCAGFFALGIAQQTKKPVAVVCTSGSALLNYHPALAEAFYSQIPFIVIS
                     ADRPKDKIDIGDGQTIRQENIYANHSLYNANLTEDASEENDVFVNEAINVAIARKGPV
                     HINVPFEEPLYETVNKLTVDFNITSLFKVYRMVGVDDIITFSTLWNHSKKILVIVGEL
                     APDAIEHKWIEKLASFPSVVVLTEANSNLHHPSFINNIDSLITPFTQSRFKKLQPRIL
                     ITFGGMIVSKRIKTFVRNYKPRHHWHLDELRAYDTFGILTHHFNVTPNQFFSQFLPYV
                     KTIDSDYKSYFAKINKTRKVKREIYLNKAPFSDLTAFSTIVKSIPKHTQLQVGNSSAI
                     RYVQLFDVDKSIDIFCNRGTSGIDGSTSTAIGAAIANKKQTVFITGDISFFYDSNALW
                     NNYIPKNFKIIVINNGGGGIFRILPGHKENKTFNTFFETSHCLTAEHIAKMYRFNYLM
                     ASDETSLNQGLVDLFANDEVPTLLEVFTPTKENDKILLQFFKELV"
     gene            5230..6462
                     /locus_tag="PK218_07925"
     CDS             5230..6462
                     /locus_tag="PK218_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW98473.1"
                     /translation="MNQTNKRKIVNDPIYGFITIPNSLIYDLIQHSYFQRLRRISQMG
                     LSYLVYPGAHHTRFHHALGAMHMMQKAIEVLRFKEVSISKDEENALLIAILLHDIGHG
                     PFSHAMEHSIVEVHHEEISMFFMHKLNQEFEGKLTLAIKIFKGEYHRKFMLQLISSQL
                     DMDRMDYLKRDSFYSGVAEGNINSDRLIQMMNVVDDVLVMEEKGIYSIEKFLTSRRLM
                     YWQAYLHKTSLVAELILTKTLKRAKELLHKGVEVDCSKPFHYFMENKITSNEFNDDTL
                     NTFSQLDDFDIISALKKWQFHDDFILSSLSKMIINRDLLKIKVSSEKFPKEELEKLTD
                     KLLENNLISKNEVDYFVFKGKIKNQAYDKAAEPIRILKKDQTIEDVVEASDQLNLKAL
                     SKPVTKYFICYPKQLLEN"
     gene            6586..7605
                     /gene="lpxD"
                     /locus_tag="PK218_07930"
     CDS             6586..7605
                     /gene="lpxD"
                     /locus_tag="PK218_07930"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPW98474.1"
                     /translation="MKFTAEQIAGILEGEVVGNPNAEVATLSKIEEGVEGSLTFLSNP
                     KYANYIYTTKATITIVNHSFVPENEISTTLIKVEDAYKSFSKLLEYYNQVKLMKSGIE
                     QPSVISENVTYGDNLYLGSFSYIGKNVKIGANVKIYPNCFIGDNVVIGDDCVFFAGVK
                     IYSETIIGNNCNIHSGSIIGSDGFGFAPNPDGSFTKIPQIGNVILEDNIDIGSCTTID
                     RATIGSTIIRKGVKLDNQIQIAHNVEIGENTVIAAQTGIAGSTKIGKNCMIGGQVGFA
                     GHLIIGDNVRIQAQSGIGKNIREGETLQGSPSFNYGDWNKSYVHFRNLPKIVAEVEEL
                     KKKIN"
     gene            7619..9007
                     /locus_tag="PK218_07935"
     CDS             7619..9007
                     /locus_tag="PK218_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HPW98475.1"
                     /translation="MVKQKTIKNEISLTGVGLHTGKEVTMTFKPAPVNNGFTFVRVDL
                     EGSPVIEADANYVVNTQRGTNLEKLGVKIQTSEHVLAALVGCDVDNVIIELNESEPPI
                     MDGSSKFFVEAIEKAEIEEQDAERIYYVVKDIISFTDEATGSEIILMPADDYQVTAMV
                     DFGTKVLGTQNATMKSISEFKNEIADSRTFSFLHELEQLLDNGLIKGGDLNNAIVYVD
                     KEISPKTMENLKVAFGKEKISVKPNGILDNLTLHYPNEAARHKLLDVIGDLALVGIKI
                     KGKVIANKPGHFVNTQFAKKLAKIIKLEQKNQIPVYDLNLTPLMDIHQIMSILPHRPP
                     FLFIDRIIEMSDSHVVGLKNVTMNESFFVGHFPGAPVMPGVIIVEAMAQTGGILILSS
                     VPDPENYLTYFMKIDNVKFKHKVLPGDTLTFKCDLISPIRRGICHMQANAYANGKLVA
                     EAELMAQIAKKQ"
     gene            9081..9863
                     /gene="lpxA"
                     /locus_tag="PK218_07940"
     CDS             9081..9863
                     /gene="lpxA"
                     /locus_tag="PK218_07940"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPW98476.1"
                     /translation="MNQPLAYVHPGAKIARNVVIDPFTTIHNNVIIGEGTWIGSNVTI
                     MEGARIGKNCNIFPGAVISAVPQDLKFGGEDSLAIIGDNCTIRECVTINRGTIASGQT
                     KIGNDCLIMATAHIAHDCHVGNNVIIVNGVLLGGHVTIGNYAIIGGLSAVHQFISIGD
                     HAMISGGSLLRKDVPPFTKAAKEPLSYVGINSVGLRRRGFTSEKIREIQEIYRILYQK
                     NYNTTQALAKIEGEMEATPERDEILDFIRNSSRGIMKGYTGV"
     gene            9899..10465
                     /gene="efp"
                     /locus_tag="PK218_07945"
     CDS             9899..10465
                     /gene="efp"
                     /locus_tag="PK218_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPW98477.1"
                     /translation="MASTSDIRNGLCIKFNHDIYKIIEFLHVKPGKGPAFVRTKLRSV
                     TNGKVLDNTFSAGHKIDVVRVETQTYQFLYAEGDTFHFMNVDNYEQITLLRDVLDAPD
                     LLKEGTNVMLQINTETDLPLSIDMPASVILEVTYTEPGLKGNTATNATKPATVETGAT
                     VNVPLFINEGDKIKIDTASGNYMERVKE"
     gene            10556..11485
                     /locus_tag="PK218_07950"
     CDS             10556..11485
                     /locus_tag="PK218_07950"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPW98478.1"
                     /translation="MKFPKIHSLKEIAAIINTTYVGADDFPVHGMNEIHVVTSGDIVF
                     VDHPKYYDKALQSAATIVLINKEVECPEGKALLISDDPFRDFNILTKYFRPFVAANVS
                     IAPSATIGENTVIQPNCFIGNNVTIGKNCLLHANVIAYDNTVIGDNVIIHSGTVLGGD
                     AFYYKKRPEGFDQLLSCGRVVIEDNVGIGALCTIDKGVTGDTTIGAGTKLDNQVHVGH
                     DTYIGKKCLIASQTGIAGCVIIEDEVTLWGQVGTTSGITIGTKAVIMGQTGVTKSVEG
                     GKSYFGTPIEESREKLKQLANVKRIPEILEKLK"
BASE COUNT         4129 a         1813 c         2041 g         3535 t
ORIGIN      
        1 caatcttcgg accagtttgt gcaaaaacaa aaccactgat aagaatcaaa atggtaagta
       61 taaatttttt cataatttgg gttttttaat atcgtaaaat tactttcttt aaattaataa
      121 gcaaaaaatt aactcttaat tttttctaaa gtctttatta attacttttg catcttaaat
      181 tacaaaaagc ataccagaaa gttttcaatg acaatacaat tacaataaac atttaaatgc
      241 caatagtaat ctgtgttctg aaatcagaaa tctaaaatct aaaatctaaa atgtcaattc
      301 cagctcaatt caacgcaaaa gaagccgaac aaaagtggta caactactgg atggagaacg
      361 attatttctc ttctaaacca gatcatagaa caccttatac catagttatt cctccaccaa
      421 atgtgacagg agtattgcat atgggacaca tgttgaacaa taccattcaa gatgtattaa
      481 ttcgtcgtgc gagattaaaa ggattcaatg cctgttgggt tccaggaact gaccatgcat
      541 ctattgccac agaagctaaa gtagtggcta aactaaaatc agaaggcatt aacaagtccg
      601 atttaacacg tgaagagttt ttaaaacacg cttacgattg gaccgataaa tatggtggaa
      661 caatactaga acaattaaaa caattaggtt gctcttgcga ttggagcaga accaagttca
      721 ctatggatga agacatgtca gcttctgtaa ttaaatcttt cgttgattta tacaagaaag
      781 ggatgattta tcgtggttac cgtatggtaa attgggatcc agaagctaaa acgacacttt
      841 cggatgaaga agtaatcttt gaagaacgcc aagggaaatt atattatatc aagtatcaaa
      901 ttgaaggtag tgaagagttt ctaactgttg caaccacccg acctgaaaca atatttggtg
      961 attcggctat ttgtattaac ccaaatgatg agcgttttac ccatttaaaa ggtaaaaaag
     1021 cgattgttcc aatttgtggt agagtagttc cgattattga agatgactat gttgatgttg
     1081 aatttggaac aggctgtctt aaagttacac cagcacacga tgttaacgat aaagaacttg
     1141 gaaacaaaca taacctagaa atcattggca tctttaatga agatgcttct ttgaattcgt
     1201 atggattaca ctatcaaggt aaagaccgtt ttgtggttcg tgatgaagct gccaaagaat
     1261 tagaagccct tggtgcttta gcaaaagttg aaaatcacat caacaaagta ggaacatccg
     1321 aaagaaccaa agcggtaata gaaccaagat tatccgatca atggttttta aaaatggata
     1381 ctttagcaaa acctgcattg aaagccgtac ttgaaacaga agaaataaaa ttatatccaa
     1441 atcgttataa caatacctac cgccattggt tagaaaatat tcgagattgg aacatttcac
     1501 gccaactttg gtggggacaa caaattcctg cctatttctt tggtgatgga aaagaagatt
     1561 ttgttgttgc tgaaactata gaagaagcat taaaattagc tcaagagaaa acttctaact
     1621 atcaacttca agcttccgat ttacgtcaag atgcagatgc attagacact tggttctctt
     1681 catggttgtg gccaatagct gttttcggag gaattcttga tcctgaaaat gaagatttta
     1741 aatactatta tccaacaaat gacttagtaa ctggtccaga tattttattc ttttgggttg
     1801 ctcgaatgat ttttgctgga tatgaatata ctggtaaaaa accttttaat aatgtatatc
     1861 taacgggttt agttcgtgac aaacaacgtc gaaaaatgtc taaatcatta gggaattcac
     1921 cagaaccgtt gcaattaatt gaaaaatttg gtgctgatgg tgttcgtgtt ggattgttgt
     1981 tgagtgcttc ggcaggaaac gacatcatgt ttgatgaaga attgtgtaac aacggtaaat
     2041 cttttgccaa taaaatttgg aatgctttcc gattaatcaa aggctgggaa gttgtcgaaa
     2101 tagtacagcc agaaagctca aaagttgcta tcgaatggta tgaagccaaa cttcaaaaaa
     2161 cacttcttga aattgaagac cattttgaaa aatacagatt gtccgatgct ttaatgtcaa
     2221 tttacaaatt ggtttgggat gatttttgtt catggttttt agaaatgata aaacctgctt
     2281 atcaacaacc aattgataaa gcgacttttg ataaagcaat tgaaatgctc gaagctaatt
     2341 tgaaattgct acatccattc atgccattct taacggaaga aatttggcaa tacatagccg
     2401 aaagaactcc aaagcaagcc ttaatagttg cagaatatcc agtaatgaca tcatttgacg
     2461 aaaagttaat tgctgatttt gaatttgcgt ctgaagttat tgcaggcata agaaccatca
     2521 gaaaagagaa aaacatttca ttcaaagata ccattgatgt aatggtaatc aataatgaaa
     2581 atagttcaac tcactttgat agcattattt tgaaacttgg aaatgtggtt gttttagaat
     2641 acataagtga agcaattgaa ggcgcattaa cgtttagagt aaaagcaaac gaatatttta
     2701 ttcctgtttc aggtgctatt gatgtagctg ccgaaattga aaaactaacg gaagaattaa
     2761 aatacactca agggtttttg cgaagcgttc aaggtaaact ttctaatgaa aagtttgttt
     2821 ctggcgcacc accacaagtc attgataatg aaagaaagaa agaagccgat gctttgtcaa
     2881 aaattgccac aatagagcaa agtctgaaaa gcttacgata agattagaaa tcccaagttc
     2941 ttcttgggat tttttattcc aataataact gcataattac cgaatgcaac catcggtcaa
     3001 acttataacc gctttctttg atgaaaccaa ccttttcaaa gccaaattgt tcgtgaaaac
     3061 gaatgctatt cacattttca gaatcaatta cgccaattat ggtgtgtagt ttttgcgctt
     3121 ttgccaattc gataagtttt tttagtaaaa gtttaccaat accttttcca tgatggtaat
     3181 tggaaacata aaccgaatgt tcaaccgtaa atttgtaggc ttcgcgaaat cggaattcgc
     3241 tgtaataacc aaaacctaca atcttattgt caacaattgc aacaataatt gggaagtttt
     3301 tattcaattt gtcttcaaaa ataattctct gctgttcaat acttctagtg ttataatcat
     3361 acaaagctgt tgaataaata atgttatagt tgattatagc tactatttct tcaagatcgc
     3421 tagggatata agttcgaatt tggattttca taaggataat aaaaatcaaa aataagaaaa
     3481 ctatcgacat ctggttaaaa ttattaaaag ttcgtacatt tgtatattaa cattaaaaaa
     3541 tgatttatcc caaaattccc ttagcacaaa gtatcattga aatttgtgcc gcaaaaggag
     3601 tacatgatat tattatttcg ccaggttcaa gaaatgcgcc attaaccatt gggtttacta
     3661 ataactcgaa atttaattgt tacagtattg ctgatgaacg ttgtgctggt ttttttgcat
     3721 tgggaattgc acaacaaaca aagaaacctg ttgcagttgt ttgtacttcg ggttctgcac
     3781 tattaaatta tcatccagca cttgcagaag ctttttacag tcaaattcca tttattgtta
     3841 tttctgccga tagaccaaaa gacaaaatcg acataggcga cggacaaacc atccgccaag
     3901 aaaatattta tgccaatcat tcgctttata atgctaactt aaccgaagac gcttcagaag
     3961 aaaacgatgt gtttgtaaat gaagcaataa atgttgcaat tgcccgaaaa ggaccagtgc
     4021 acatcaacgt tccatttgag gaaccattat atgaaacggt aaacaaatta acggtcgatt
     4081 ttaatatcac cagtttattc aaagtgtata gaatggttgg agttgatgat attatcacgt
     4141 tttcaacttt atggaatcac agtaaaaaaa tattagtcat tgttggagaa ttagctccag
     4201 atgcgataga acacaaatgg attgaaaagt tagcgtcatt tccatcggtt gttgtgctaa
     4261 ctgaagccaa ctcaaatttg catcatccaa gttttataaa caatattgat agcttaataa
     4321 caccatttac ccaaagtaga tttaaaaaat tacaacccag aattttaatc acttttggcg
     4381 gaatgatagt ttccaagcgt atcaaaacat tcgttagaaa ctataaacca agacatcatt
     4441 ggcatcttga tgaattaaga gcttatgata ctttcggaat tttaacgcat catttcaatg
     4501 taacgccaaa tcaatttttc tctcagtttt tgccctatgt aaaaacaata gatagtgatt
     4561 ataaaagcta ctttgcaaaa atcaataaaa ctcgaaaagt caaacgtgaa atctatctga
     4621 ataaagcacc ttttagcgat ttaacagctt tcagcacaat agtaaaatca attccaaaac
     4681 acacgcaact tcaagtagga aatagttcgg ctattcgata tgtgcaattg tttgatgttg
     4741 ataaaagcat cgatattttt tgtaatcgtg gcactagcgg aatagatgga agtacttcaa
     4801 cagcaatagg tgctgcaatt gccaataaaa aacaaactgt tttcattact ggcgacataa
     4861 gtttctttta tgacagcaat gcgctttgga acaattatat tcctaaaaat tttaaaatta
     4921 ttgtgattaa taatggtggt ggtggtattt ttagaatcct tccaggtcac aaagaaaata
     4981 aaaccttcaa tacttttttc gaaacatctc attgtttaac cgccgaacat atagctaaaa
     5041 tgtaccgttt taattattta atggcaagcg atgaaacttc tctaaatcaa gggttagtag
     5101 atttgtttgc aaatgatgaa gtaccaacat tattagaagt atttacacca accaaagaga
     5161 atgataaaat attgttacaa ttcttcaagg aattagtcta atatttttga ccttaaacat
     5221 aaatttagag tgaaccaaac aaataaacga aaaattgtca atgatccgat ttatggtttt
     5281 attaccattc caaattcatt gatatacgat ttaatacagc attcttactt tcaacgactt
     5341 cgacgtattt ctcaaatggg attgtcgtat ttagtctatc ctggtgcaca tcatacccgt
     5401 tttcatcacg cattgggcgc tatgcacatg atgcagaaag caattgaagt acttcgtttt
     5461 aaggaagtgt ctatttctaa agatgaagaa aatgctttgt taattgcaat tttattgcac
     5521 gatattggtc atgggccatt ttctcatgcc atggaacata gtattgtaga agttcatcac
     5581 gaagagattt ctatgttttt tatgcataaa ttaaatcaag aatttgaagg aaagttaacg
     5641 ttagccataa aaatattcaa aggtgaatac catagaaaat tcatgttgca attgatttct
     5701 agtcaattgg atatggatag aatggactat ttgaagcgag atagttttta ttcgggtgtt
     5761 gctgaaggaa atattaattc cgatagattg attcaaatga tgaatgttgt tgacgatgtg
     5821 ttagtcatgg aggaaaaagg aatctattct attgaaaaat tcttaacatc taggcgattg
     5881 atgtattggc aagcttattt gcacaaaaca agcttagtag ctgagttaat cttaaccaaa
     5941 acattaaaaa gagctaaaga acttttgcat aaaggagtag aagttgattg tagtaaacct
     6001 ttccattatt tcatggaaaa taaaattact tcaaatgaat ttaatgatga cacactaaat
     6061 acattttcgc aattggatga ttttgatatt atttctgcac taaagaaatg gcagttccac
     6121 gatgatttta ttttaagcag tttgagtaaa atgattatca atcgcgattt attgaaaatt
     6181 aaagtaagtt cagaaaaatt tcctaaagaa gaattagaaa aactgaccga taaacttctt
     6241 gaaaacaatt taatttctaa aaacgaagta gattattttg tgttcaaagg aaaaataaaa
     6301 aaccaagcct atgacaaggc agctgagcct atcaggatat tgaaaaaaga ccaaacaatt
     6361 gaagatgttg ttgaagcatc tgaccaacta aatttaaagg ctttgtcaaa accagttact
     6421 aagtatttta tatgttatcc aaaacaactc ttagaaaact aatattaaaa ttattttttt
     6481 atatttttgt gccaatgaat acaaatttta caaatttctc ggtgttttta aaagactgtt
     6541 tgcaaaccag tataatttca tgtcgaatag aaaaattact ataagatgaa gtttacagca
     6601 gaacaaattg caggcatact tgaaggagaa gtagttggga atccaaatgc tgaagtagct
     6661 actttgtcta aaattgaaga aggcgttgaa ggttctttaa cttttttatc aaatccaaaa
     6721 tatgccaatt atatatatac tacaaaagca acaataacta tagttaatca ctcttttgtt
     6781 cctgaaaatg aaatatctac aactttaatt aaagtggaag atgcttataa atctttttct
     6841 aaattattag aatactataa tcaagttaaa ttgatgaaat caggcataga acaaccttct
     6901 gttatttcag aaaacgttac ttatggcgat aatttatatt taggaagttt tagttatatt
     6961 ggtaaaaacg ttaaaattgg agctaatgta aaaatatatc ccaattgttt tattggcgat
     7021 aatgtcgtta ttggtgatga ttgtgttttc tttgctggtg ttaaaattta ttcagaaact
     7081 atcataggaa ataattgcaa cattcattct ggttcaataa ttggttctga tgggtttggt
     7141 tttgctccaa atccagatgg aagttttact aaaattccac aaattggtaa tgtaatttta
     7201 gaagacaaca tagatattgg ttcatgcact acaattgata gagctacaat aggctcaacg
     7261 attattagaa aaggagtaaa gctagacaat caaattcaaa ttgctcacaa tgttgaaata
     7321 ggtgaaaata ctgtgatagc agctcaaaca ggaattgctg gttcgaccaa aattggtaaa
     7381 aactgtatga ttggaggaca agttggtttt gcgggtcatt taatcattgg cgacaatgtt
     7441 cgcattcaag cacagtctgg aatcggaaag aacattagag aaggtgaaac gcttcaaggt
     7501 tctccgtcgt tcaattatgg cgattggaat aaatcgtatg tacattttag aaatttacct
     7561 aaaattgttg ctgaagtaga agaattaaag aaaaaaataa attaaaactg aatatccaat
     7621 ggttaaacaa aagaccatca aaaacgaaat ttccctaaca ggagttgggc ttcacacagg
     7681 taaagaagtt accatgactt tcaaacctgc accagtaaac aatggtttta cattcgtacg
     7741 tgttgattta gaaggaagtc cggtaattga agctgatgct aattatgtgg tcaacacaca
     7801 gcgtggtact aatctagaaa aacttggtgt aaaaattcaa acctcagagc atgtattagc
     7861 agctttagta ggttgtgatg ttgataatgt tattatcgaa ttgaatgaat ctgaaccgcc
     7921 aattatggat ggttcttcta aattttttgt agaagcaatt gaaaaagcag aaatagagga
     7981 acaagatgct gaacgtattt attacgttgt caaggacatc atctcattta ctgatgaagc
     8041 tactggaagc gaaattattc ttatgccagc cgatgattat caagtaactg ccatggttga
     8101 ttttggaact aaagttttag gaactcaaaa tgcaactatg aaaagtattt cagaattcaa
     8161 aaatgaaatt gcagattcca gaacttttag ttttttacat gagttagagc agcttttgga
     8221 caacggatta ataaaaggtg gcgatttaaa taatgctatt gtgtatgttg ataaagagat
     8281 ttctccaaaa acaatggaaa acttaaaagt agcttttggt aaagaaaaaa tatccgtgaa
     8341 acctaatggt attcttgata atcttacttt gcactatcca aacgaagctg caagacacaa
     8401 attgttagat gtaattggtg atttggctct agttggaatt aaaattaaag gaaaagtaat
     8461 agcaaataaa ccaggtcatt ttgtaaatac tcaatttgca aaaaaattag ccaaaatcat
     8521 caaattagaa caaaaaaatc agattccagt ttatgattta aatttaacac ctttaatgga
     8581 tattcatcaa attatgagta tcctaccaca cagaccacca tttttattta ttgatagaat
     8641 tatagaaatg tctgatagtc atgtagtggg attaaaaaat gttacaatga atgaaagctt
     8701 tttcgttgga cattttccag gtgctccagt aatgccaggt gttattatag tggaggctat
     8761 ggctcaaacc ggtggaattt taattctaag ttcagtgcct gatcctgaaa attacttgac
     8821 ctattttatg aaaattgata atgtaaaatt caaacacaag gtattgccag gagatacttt
     8881 aacatttaaa tgcgatttaa tttccccaat tcgcagagga atttgtcaca tgcaagccaa
     8941 tgcttatgca aatggaaagt tagtagcaga agctgaatta atggcgcaaa ttgccaaaaa
     9001 acaataataa aaaaggaggg taggctatag gcggtaacta aaactacaac ctacagccca
     9061 caacctaaaa cctaatacat atgaatcaac ctttagcata cgttcatcca ggagcaaaaa
     9121 ttgcacgaaa tgtagtgatt gatcctttta ctaccattca taacaatgtc attattggcg
     9181 aaggaacttg gataggttca aatgtaacca taatggaagg agctagaatc ggtaaaaatt
     9241 gcaacatttt tcctggtgct gttatctcgg cagtacctca agatttaaag tttggtggcg
     9301 aagattcatt agcaatcatt ggcgataatt gcaccattag agagtgtgtt actatcaata
     9361 gaggaacaat agcttctgga caaactaaaa taggtaatga ttgtttaatc atggcaacgg
     9421 cacatattgc tcatgattgt catgttggca ataatgttat tattgtaaat ggcgttcttc
     9481 ttggcggaca tgtaaccatt ggaaactatg ctattattgg cggtttgtcg gctgttcacc
     9541 aatttattag tattggtgac catgccatga tttcgggtgg ttcattatta agaaaagacg
     9601 ttcctccgtt tacaaaagca gcaaaagaac cactttccta tgttggaatc aactctgtag
     9661 gtcttagaag aagaggattt acatcagaaa aaattagaga aattcaagag atttatagaa
     9721 tattgtatca aaaaaattat aatacaacac aagctttagc taagattgaa ggcgaaatgg
     9781 aagcaacacc tgaacgtgat gaaattctcg acttcatcag aaactcatca agaggtatca
     9841 tgaaaggata cacaggagta taagtaacaa ataaacgaat aaccaaataa acaacaatat
     9901 ggcatctaca tcagatatta gaaacggatt atgcatcaag tttaaccacg atatttataa
     9961 aattattgaa tttcttcacg taaaacccgg aaaaggtcca gcattcgtga gaaccaaact
    10021 cagatctgta accaacggaa aagttttaga caatactttc tctgcaggac acaaaataga
    10081 cgtcgttcgt gtagaaacgc aaacctatca attcttgtat gctgaaggcg acacttttca
    10141 tttcatgaat gtagataact acgaacaaat cacgcttcta agagatgtac ttgatgctcc
    10201 agacttatta aaagaaggaa ctaacgtaat gctacaaatc aataccgaaa ccgatttacc
    10261 gctttctatc gacatgcctg cttctgttat tcttgaagtt acctatactg aaccaggttt
    10321 aaaaggaaat actgctacca atgctactaa accagcaaca gtagaaacag gcgcaaccgt
    10381 aaatgttcct ctatttatca acgaaggaga caaaatcaaa attgatacag cttctggaaa
    10441 ctatatggaa agagtaaaag agtaattaat ttgaaaatgt ggtaatttga agatttgaaa
    10501 attatttttc actcactttc aaatttccaa attttcaaat tttcaaatca aataaatgaa
    10561 gtttcctaaa atacattccc taaaagaaat cgcagccatt attaacacta cttatgttgg
    10621 tgcagatgat tttccggtgc acggtatgaa cgagattcat gtcgttacct ctggcgatat
    10681 agtgtttgtt gatcatccta agtattacga caaagcatta caatctgcgg ctactattgt
    10741 tttaattaac aaagaagtag aatgtccaga aggcaaagca ttgttaatta gtgacgatcc
    10801 atttagagac ttcaatatat taaccaaata ttttcgtccg tttgtagcag caaatgtgag
    10861 tattgctcca tcggctacca ttggcgaaaa cacggtcatt caacccaatt gttttattgg
    10921 aaataatgtt accataggca aaaactgttt gctacatgcc aacgtcattg cctatgataa
    10981 cacagtcatt ggcgataacg tcatcatcca ttctggaaca gtactaggag gagatgcttt
    11041 ctattataaa aaacgtcccg aaggctttga tcaactgctt tcttgcggaa gagttgtcat
    11101 cgaagacaat gttggaatag gtgctttatg taccatcgat aaaggtgtta caggcgatac
    11161 taccattggt gctggaacaa aattagacaa ccaagtacat gttggtcacg atacctatat
    11221 tggcaaaaaa tgcctgattg cttcacaaac cggtattgct ggttgcgtga ttatagaaga
    11281 tgaagtaacg ctttggggac aagtaggtac cacgagtggc atcactatcg gaactaaagc
    11341 cgtaatcatg gggcaaacag gtgttactaa atccgtagaa ggaggaaaaa gttattttgg
    11401 cacaccaata gaagaatctc gcgaaaagct aaaacaattg gccaacgtaa aacgtattcc
    11461 tgaaatctta gaaaaattaa aataactatg aaagccaaag acattgttgc caatttct
//
LOCUS       DAOOBH010000071        11277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_7342_length_11277_cov_4.968544, whole genome shotgun sequence.
ACCESSION   DAOOBH010000071 DAOOBH010000000
VERSION     DAOOBH010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7342_length_11277_cov_4.968544"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PK218_07955"
     CDS             complement(<1..243)
                     /locus_tag="PK218_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HPW98479.1"
                     /translation="MKHDIQNREDIIKLVDVFYDKVKKDNVIGYIFSDIAHVNWDLHL
                     PRMYDFWENILFYTGNFDGNPMMTHKTLNEKAPMDFT"
     gene            complement(261..1028)
                     /locus_tag="PK218_07960"
     CDS             complement(261..1028)
                     /locus_tag="PK218_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPW98480.1"
                     /translation="MNRIIKIILLDILKNKIVLAYTLIIAVLAWSSFALEDSSSKGLL
                     TILNVILFTVPLVSILFATIYLYNSSEFIELLLSQPIKRAKIWISLFLGLSLSMVIAF
                     FVGAGIPLLIFASNAVGFMMILVGCIISVVFIALAFLSSIVTRDKAKGIGIAIMTWLF
                     FALLFDGIVLFLLFQLSDYPIENAMVGVTAFSPIDLARIQILLHLDVSAMMGYTGAIF
                     KDFFGTTVGLFVSFLLLILWAIIPFTVSLLKFKNKDL"
     gene            complement(1021..1734)
                     /locus_tag="PK218_07965"
     CDS             complement(1021..1734)
                     /locus_tag="PK218_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW98481.1"
                     /translation="MITIQNINKRFGKLEVLKNVNLTCNKGECIALIGPNGCGKTTLI
                     KAILGMVLPDNGTIKFNEQNIKNEYLYRNKIGYMPQIGRYPDTMTIGNIIEMIKKIRN
                     SNDALDEDLIKAFELEKMFDKQMRTLSGGTTQKVSATLAFLFNPEVLILDEPTAGLDP
                     LASELLKEKIIKEKEKGKLILITSHLLSELDDLITEIIFMQEGKVHFHTKVADLLKET
                     NETKISRAIASVLKNKKDE"
     gene            complement(1731..2966)
                     /gene="nosD"
                     /locus_tag="PK218_07970"
     CDS             complement(1731..2966)
                     /gene="nosD"
                     /locus_tag="PK218_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HPW98482.1"
                     /translation="MKYLLKIVFLLFFGVVVQGQTIDVGANQKIKTIKQAISIANAGD
                     TIKIQKGTYREGTIIVDKKVVIIGVDYPVLDGQKKYEILSIKHDSVVVKGLKLIRSSF
                     ASMEDPAAIKVYNVNTIHIENNILDDNFFGIYIQNGRNCIIKNNHLRAYGKEEQKLGN
                     GIHCWKSNDIQIIANIIIGHRDGIYFEFVFDSVIWRNKSENNIRYGLHFMFSHNDSYI
                     TNIFRNNGAGVAVMYSKEVKMINNLFEKNWGQSAYGLLLKELNDGYILGNKFIQNTAG
                     IYMEGSNRMQLKNNLFQENGWAMKIQASCDDNNIVNNNFIGNTFDMGTNGSLVLNNFD
                     SNYWDKYEGYDLDKNGLGDVPYHPLSLFAVLSENNPSTMLLYRSFMITLLDKSEKILP
                     TITPDNFIDNKPLMKSLPL"
     gene            complement(2982..3995)
                     /locus_tag="PK218_07975"
     CDS             complement(2982..3995)
                     /locus_tag="PK218_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HPW98483.1"
                     /translation="MNTSKISGVSKGLLLIVGCLFIASLFYPMWQIELEAPQYPEGLV
                     LKLHANKIGGDVEIINGLNHYIGMATLHTENFIEFTVLPYIFAAFALISIVSLLVARR
                     KMVLYFLIIYVLFIVLAAIDFYRWNYEYGHNLDPNAAIKVPGMAYQPPLIGYKQLLNF
                     GAYSVPDIGGWMLTLAGILLFAVVAMEFKLFKRFQKNTKATLLILLTFGFFLSCSTKD
                     MEPIKLNVDQCSFCKMTIADGKFGAEIITKKGRVYKFDDLHCMKGYLSENKTDVKSYF
                     VHDYTKENILIPAEKAFYLKDGAISSPMRGNIAAFVDENEASAFQSKLNAQPISFEEI
                     SAN"
     gene            complement(4053..6032)
                     /gene="nosZ"
                     /locus_tag="PK218_07980"
     CDS             complement(4053..6032)
                     /gene="nosZ"
                     /locus_tag="PK218_07980"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HPW98484.1"
                     /translation="MNKNNIIKAFFAIIIASALFTSCKPKNSNDAVSGDAAQKAYVAP
                     GKYDEFYNFVSGGFSGQMSVYGLPSGRMLKVIPVFSADPESGWGYNEETKPMLNTSHG
                     FIPWDDAHHPEMSQTNGEVDGRWVFINGNNTPRIARIDLKRFKTAEIIELPNSAGNHS
                     SPFITENTEYVVAGTRFSVPGDYEDGDVPINTYKQNYKAHISFVKVGKEGEMDIAFQL
                     RLPGVDFDLSHAGKGKSHGWFFFSCYNSEQANTLLEVNASQKDKDFIMAVNWKKAEEY
                     IKAGKGKREKTKYAHNYWDEKTHSAVSEMKEDVLVLDAMALKDICYMIPCPKSPHGCD
                     VDPSGEYICGSGKLAALIPVFSYTKMLKAIETKNYEGDYQGIPVLKYDAVLHGEVQKP
                     GLGPLHTEFDGKGNAYTTMFVSSEVVKWDIKTLKVLDRVPTYYSVGHLMIPGGDSRKP
                     FGKYMVAYNKITKDRYLPTGPELTQSAQLYDISGDKMQLILDFPTIGEPHYAQAAPAD
                     LIRNNGQLKFYKIEDNQHPFVTKGEKESKVVREGNKVHVYMTCTRSHFAPDNIEGIKV
                     GDEVYFHVTNLEQDWDVPHGFAVKGAKTAELLIMPGETATLKWIPEKVGTTVFYCTDF
                     CSALHQEMQGYLRVSPKGSSVPITYSLGTNLPKEK"
     gene            complement(6044..6517)
                     /locus_tag="PK218_07985"
     CDS             complement(6044..6517)
                     /locus_tag="PK218_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPW98485.1"
                     /translation="MNKSILVSLSFALLMACGKKEKENEEYNPSATTTEATTDVSSYD
                     PNRGEGKFHNVEIAATLDNAMATKGEEVAGVKCISCHKTTDEKLVGPGWKGVTERKKP
                     EWIMNFITNPDPMIDKDPEVQAQLEICLVRMPNQGLNDEEARSILEYMRKIDGVK"
     gene            6677..7084
                     /locus_tag="PK218_07990"
     CDS             6677..7084
                     /locus_tag="PK218_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98486.1"
                     /translation="MKKLIITALLVVSFTTFAQGKKGHKGDREKLTTEQKVDLQVKKM
                     TSELALNETQVKEIRTLIEKEVAKRDEKKREDRKAASKEKKAEMKEEQARISAEMKTI
                     LTTEQYSKWEKMKEERKEKMKEKMQERKEKKQN"
     gene            7177..7545
                     /locus_tag="PK218_07995"
     CDS             7177..7545
                     /locus_tag="PK218_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98487.1"
                     /translation="MKKIVFVLTAIIAFSFSSNAQNKKELSKEDMVKQEQLKKESPEV
                     NAKNDAVELSKYLGLDDTMTNDFYRLFEMKYKTLQNELTPERKTELSRVMDAKIRATI
                     GDAKMEKLESNEALYKRLIN"
     gene            complement(7597..8883)
                     /gene="hemL"
                     /locus_tag="PK218_08000"
     CDS             complement(7597..8883)
                     /gene="hemL"
                     /locus_tag="PK218_08000"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPW98488.1"
                     /translation="MLYQRSSQLFAEAEKVIPGGVNSPVRAFKGVGGTPIFAKSAKGA
                     YVYDEDNNRYIDYINSWGPMILGHAFQPVVDAVIEKTKLGTSFGMPTELETKIATLAV
                     SMVPNIDKIRFVSSGTEACMSAIRLARGFTKKDKIIKFAGCYHGHSDSFLIQAGSGAS
                     TFGTPNSPGVTQGTAKDTLLANYNDIENVKALFESNKNEIAAIIIEPVAGNMGCVPPT
                     DGFLQNLRAVCDANNSLLIFDEVMTGFRLAKGGAQELYNVAADLVCFGKVIGGGLPVG
                     AFAARNEIMNHLSPLGSVYQAGTLSGNPLAMAAGYAMLQSLNKDITIFKRLEEKTAYL
                     HKGIETVLTQNKIEFTINRVGSMISVHFDENPVFDFNSAKNGDNDRFKKFFHGLLQEG
                     VYIAPSAYETWFISDALTYEDLDFTIQAIDTVSKQL"
     gene            complement(8904..9794)
                     /locus_tag="PK218_08005"
     CDS             complement(8904..9794)
                     /locus_tag="PK218_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HPW98489.1"
                     /translation="MHTKFLIGFLLIFLVSCSSSKPVVRTSKSSKTVVRKPIAHNSSK
                     SKVVTSRPNNTSTSGSNNSSSSQNNDISTVVLEATTSVKVTTKLVLEYIDTFKEIAKD
                     NMVKNGIPASITLGQAILESGAGTGPLSAQANNHFGIKCHKEWDGPSIKYTDDAEDEC
                     FRKYNDPSESFRDHSYFLTSRSRYADLFKLDKDDYKRWAKGLKAAGYATDPKYPDKLI
                     SLIERYDLQRFDAEVLGKDYETNPNSQVAYQDPSKYTVVKGDTLYSISKRFNISIEDL
                     KKKNNLSDTNLSLGQRLHVN"
     gene            complement(9784..10674)
                     /locus_tag="PK218_08010"
     CDS             complement(9784..10674)
                     /locus_tag="PK218_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPW98490.1"
                     /translation="MYQTIIIKNKLLSIKREDLLHPYISGNKFRKLKYNIEEVKRLNK
                     NTLLTFGGAYSNHILAVAAVGKEYGFKTIGIIRGEELVNNIENNPTLSKAKEFGMEFD
                     FISRDNYRNKNDENFIATLKLKFGDFYLLPEGGTNLLAIKGCEEIVTSEDDAFDYICC
                     AVGTGGTISGVINCSKHCQKVLGFPALKGDFLQEDIRKFANKENWELINDYHFGGYAK
                     TTNELIDFMNEFYTQYQIPLDPIYTGKMVFGVFDLIKKNYFPENAKIRILHTGGLQGI
                     NGMNLVLKNNNLTQIKYNAY"
     gene            10856..>11277
                     /locus_tag="PK218_08015"
     CDS             10856..>11277
                     /locus_tag="PK218_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HPW98491.1"
                     /translation="MKKKYYLLVFAFIFIKSNLLYSQTNCSTALPITINGACQSGSVN
                     DNSADLPALTGCTATFNRERWYTFTVVGGSQTITITGTSTSTNRNLYLQLISLTGTCA
                     SGTLTQIACANAINTNSAQTETITITLTNGVYFIKVLNV"
BASE COUNT         3803 a         2058 c         1581 g         3835 t
ORIGIN      
        1 cgtaaaatcc ataggtgctt tttcgttcaa cgttttgtgt gtcatcatcg gattgccatc
       61 aaaatttcct gtataaaaaa gaatgttttc ccaaaaatca tacattctcg gaagatgcaa
      121 atcccagttt acatgagcaa tatcactaaa aatataccca ataacgttat cctttttaac
      181 cttatcataa aagacatcta caagcttgat aatgtcttcc ctattttgaa tatcatgttt
      241 cataacggta aaattattaa ttataaatct ttgtttttaa acttcaataa cgaaacagta
      301 aacggaataa ttgcccacag aatcaacaat aggaacgaaa caaataaacc aacagtagtt
      361 ccaaaaaaat ctttaaatat tgcaccagta taacccatca tcgcagatac atccaaatgc
      421 aataatatct gaattcgtgc caaatcaata ggactaaaag cagtaacacc caccatggca
      481 ttctctattg gatagtctga aagttgaaac aataaaaaaa gtacaatacc atcaaaaagc
      541 aaagcaaaaa acaaccaggt catgatagca attccaattc ctttggcttt atcacgcgtt
      601 acaatagaac ttaaaaaagc caacgcaata aacaccaccg atataataca acctaccaaa
      661 atcatcataa acccaacagc gtttgaggca aaaattaaca gcggaatgcc agcacctaca
      721 aaaaaagcaa tcaccataga caatgaaagc ccaagaaata ggctaatcca aatcttcgct
      781 cttttaatcg gttgactcaa taataattca ataaactcag aactattata aagatatatc
      841 gttgcaaaaa gaatagaaac caatggcacc gtaaacaata taacattcaa aatagtcaac
      901 aatcctttag acgaactatc ttctaaagca aacgaactcc acgccaaaac cgctataatc
      961 aaggtataag ccaacacaat tttgtttttc aagatgtcca acagtatgat tttaattatt
     1021 ctattcatct tttttatttt ttaaaacact agcaatcgct ctagagattt tagtttcgtt
     1081 agtttctttc aacaaatcgg caactttagt atgaaaatga actttgcctt cttgcataaa
     1141 aataatttcg gtaatcaaat catccaattc actcaataaa tgcgacgtaa taagaatcaa
     1201 ttttcctttt tctttttcct taataatctt ttctttcaac aattcagaag ctaagggatc
     1261 taaaccagcc gtgggttcat caagaatcaa tacctctgga ttgaacaaaa aagccaacgt
     1321 agcactcact ttttgagtag ttccacccga aagtgttcgc atttgtttat caaacatttt
     1381 ttcgagttca aacgctttta ttaaatcttc atccaatgca tcgttagaat ttctaatttt
     1441 cttaatcatt tcgatgatat ttccaatagt catcgtgtca ggataacgac caatttgagg
     1501 catgtatcca attttgtttc tgtataagta ttcgttttta atgttttgtt cgttaaactt
     1561 aatcgttccg ttatcgggca aaaccatacc tagaattgct ttgattaagg ttgtttttcc
     1621 gcaaccattt ggtccaatta aagcgatgca ttctccttta ttacaagtaa gatttacatt
     1681 ctttaatact tctaatttcc caaatctctt gttgatgttt tgtatcgtta tcataaaggc
     1741 agtgacttca ttaaaggctt attatctata aaattatcag gcgttattgt aggtaaaatt
     1801 ttctccgatt tgtccaaaag cgtaatcata aaactgcgat agagcaacat agttgaagga
     1861 ttattttcag ataaaaccgc aaacaaactc aaaggatgat agggcacatc gccaagtccg
     1921 tttttgtcta aatcatagcc ttcgtattta tcccaataat tgctgtcaaa attgtttaaa
     1981 accaaactac cattggtacc catatcaaaa gtattcccga tgaaattatt gttcacaata
     2041 ttgttgtcat cgcaactggc ttgtatcttc attgcccaac cattctcttg aaacaaattg
     2101 tttttcaact gcattctatt agaaccttcc atataaattc ctgccgtatt ttgaataaat
     2161 ttgttgccaa gtatatagcc atcattcagt tcttttaaca aaagtccata cgccgattgt
     2221 ccccagtttt tttcaaacaa attattaatc attttaactt ctttgctata cataacggca
     2281 actccagcac cattatttct aaagatatta gtgatatacg aatcgttgtg cgagaacata
     2341 aaatgcaatc cataacggat attgttttcg cttttattgc gccaaatcac agaatcaaaa
     2401 acaaactcaa aataaatgcc gtcacgatgc cctataataa tgttcgctat gatttgaata
     2461 tcattgcttt tccaacaatg gatgccgttt ccaagttttt gttcttcttt tccataagct
     2521 ctaagatgat tgtttttgat gatacaattt cttccatttt gaatgtaaat cccaaaaaaa
     2581 ttatcatcta aaatgttatt ctcaatatga atagtattga cattatacac ttttattgcc
     2641 gcaggatctt ccattgaggc aaaagacgaa cgtattaatt ttaatccttt aaccacaacg
     2701 ctatcatgct ttatggataa gatttcatac tttttctgac catccaaaac aggataatcc
     2761 acaccgataa tcactacttt tttatctaca attatagttc cttctctata cgttcctttc
     2821 tggattttta tagtatcacc agcgtttgca atagaaatcg cttgtttgat tgtttttatt
     2881 ttttggttcg cgccaacatc aatagtttga ccttgaacaa caacaccaaa aaaaagtaaa
     2941 aaaactattt tgagtaaata tttcatcgaa agtttttcta attaattagc actaatttct
     3001 tcaaatgaaa ttggttgtgc atttagtttt gattgaaaag cactggcttc gttttcatct
     3061 acaaatgccg caatatttcc tcgcatcgga ctcgaaatag caccatcttt tagataaaat
     3121 gctttttcag ctggtattaa aatattctct ttagtataat catgcacaaa ataagatttg
     3181 acatcagttt tattttcaga caaatagcct ttcatacaat gcaaatcatc aaatttataa
     3241 actcttcctt ttttggtaat gatttctgca ccaaatttcc catcagcaat agtcatttta
     3301 caaaaagaac attgatcaac atttaacttt attggctcca tatctttagt gctacaagac
     3361 aagaaaaagc caaaagttaa gagtattaga agtgttgctt tcgtgttttt ttgaaaacgt
     3421 ttaaacagtt taaactccat tgcaacaaca gcaaatagaa gaattcctgc caatgtgagc
     3481 atccatccac caatatctgg aacagaataa gcaccaaaat tcaataattg tttgtatcca
     3541 attaatggcg gttgataagc cattcctggt actttaatcg cagcatttgg gtctaaatta
     3601 tgaccgtatt catagttcca tctgtaaaaa tcgatagccg ctagaacaat aaataatacg
     3661 tagataatta aaaaatacaa caccattttt cttctagcaa caagtaagga tacaattgaa
     3721 attagagcaa aagcagcgaa gatatagggc aaaacagtga attcgataaa attttcagtg
     3781 tgtaaggttg ccatcccaat ataatggttt aatccgttga tgatttctac atcaccacca
     3841 attttattag catgcagttt caacactaaa ccttctggat attgtggagc ttctaattct
     3901 atttgccaca ttggataaaa caaagaagca ataaacaagc aaccgactat caaaagtaac
     3961 ccttttgata ctccagaaat ttttgaagta ttcataacat taattttttt aagaaaggga
     4021 acaatgaaaa ccactgttcc ctctttgaag atttattttt cttttggaag gtttgttcct
     4081 aagctataag taattggaac agaactacct tttggagaca ctctcaaata gccttgcatt
     4141 tcttggtgaa gtgcactaca gaaatcggta caataaaaca ctgttgttcc tactttttct
     4201 ggaatccatt ttagagtagc cgtttctcct ggcatgatta acaactcagc ggttttggca
     4261 ccttttacag caaacccgtg aggaacatcc caatcttgtt ctaaattagt aacgtgaaag
     4321 taaacttcat cacctacttt aatcccttcg atattatcag gcgcaaagtg agaacgagta
     4381 caagtcatgt aaacatgaac tttatttcct tcgcgaacca cttttgattc tttttcgcct
     4441 ttagtaacaa aaggatgttg attgtcttca attttataga attttaattg tccattattt
     4501 ctaattaaat cggctggagc ggcttgagca taatgaggtt cgccaatcgt tggaaaatct
     4561 aatatcaatt gcattttatc tcccgaaata tcatataact gagctgattg cgttaattct
     4621 ggtcctgttg gcaaatacct gtccttagta attttgttat aagcaaccat atattttcca
     4681 aatggctttc tactatcacc accaggaatc attaagtgtc caacagaata ataagtagga
     4741 actctatcta aaacttttag tgttttgata tcccatttaa ccacttctga tgaaacaaac
     4801 attgtagtat aggcatttcc tttaccatca aactcagtgt gtaatggtcc taaaccaggt
     4861 ttttgaactt caccgtgaag tactgcatca tatttaagaa ctggtattcc ttgataatcg
     4921 ccttcatagt ttttagtttc gatagctttt agcattttag tgtaactaaa taccggaatt
     4981 aaagcagcta attttccgct accgcaaata tattctccag atggatctac atcacaaccg
     5041 tgaggagatt ttggacaagg aatcatgtag caaatatctt ttaaagccat tgcatctaga
     5101 actaatacat cttctttcat ttcagaaacg gcagaatgtg ttttttcatc ccagtaattg
     5161 tgagcatatt tggttttttc tctttttcct ttacctgctt tgatatactc ttcggctttt
     5221 ttccagttta cagccatgat aaaatcttta tctttttgtg aagcgtttac ttctaaaagt
     5281 gtattagctt gttctgaatt gtagcaagaa aagaaaaacc atccatgcga ttttccttta
     5341 ccagcatgac ttaaatcgaa atctacaccc ggtaatcgca attgaaaagc aatatccatt
     5401 tcgccttctt tacccacttt tacaaaacta atatgcgctt tataattttg tttgtatgta
     5461 ttgataggta catcaccatc ttcataatca cctggcacac taaaacgagt accagccaca
     5521 acatattctg tgttttcagt aataaatggt gaagagtgat ttcctgcact atttggtaac
     5581 tcaataattt ctgctgtttt aaaacgtttt aaatcaattc gagcaatacg tggtgtatta
     5641 tttccattga taaaaaccca acgcccatct acttcaccat tggtttgaga catttctgga
     5701 tgatgagcat catcccaagg aataaatcca tgcgaagtgt ttaacattgg tttggtttct
     5761 tcattataac cccaaccact ttctggatcg gcagaaaaaa caggaattac ctttaacatt
     5821 cttccacttg gaagtccata aacgctcatt tgaccgctaa atccacctga aacgaaattg
     5881 tagaattcat catatttacc cggtgctaca taggcttttt gagcagcgtc accgctaacg
     5941 gcatcatttg aattcttggg tttacaagat gtaaacagag cacttgcaat tataattgca
     6001 aaaaatgctt ttattatatt atttttattc atgataaagt tatttatttt acaccatcaa
     6061 ttttacgcat gtattctaaa atacttctag cttcttcgtc gttcaaacct tgatttggca
     6121 tacgaaccaa acaaatttct aattgtgctt gtacttctgg atctttgtca atcattgggt
     6181 caggattggt gataaaattc attatccatt ctggcttttt acgttctgta acgcctttcc
     6241 aacctggacc aactaatttt tcgtccgttg ttttatgaca agaaatacat tttacacctg
     6301 caacttcctc tccttttgtt gccatagcat tgtctagagt ggcagcaatt tcaacattat
     6361 gaaatttacc ttctcctcgg tttggatcat aagacgaaac gtctgttgtg gcttcagttg
     6421 ttgtggctga tggattgtat tcctcatttt ctttttcttt cttaccacat gccattaata
     6481 gggcaaatga taatgatact aaaattgatt tgttcatgat aaattatgtt taaatttctt
     6541 atacaaaatt ctttaataat gcattaaaat tttatgattt caatcatact tcatgattta
     6601 atactaaaat tttgttaaag aataaacctt ttaataaact caaagtctaa agtgaaatca
     6661 ttaaaattaa atactcatga aaaaattaat tattaccgct ttattagtgg ttagttttac
     6721 aacttttgca caaggtaaaa aagggcacaa aggtgacaga gaaaaactga caacagaaca
     6781 aaaagttgat ttacaagtaa aaaaaatgac aagtgaattg gctttaaatg aaactcaagt
     6841 caaagaaatt cgtactttaa ttgaaaagga agtagcgaaa agagatgaga aaaaaaggga
     6901 agacagaaaa gccgcgtcaa aagagaaaaa agctgaaatg aaagaagaac aagccagaat
     6961 atctgctgaa atgaaaacca ttttaactac tgaacaatat tctaaatggg aaaaaatgaa
     7021 agaggaacgc aaagaaaaaa tgaaagaaaa aatgcaagaa cgaaaagaga aaaaacagaa
     7081 ttaaaactaa ttgttgattt attttcgaaa taaattttga gttgatttaa aaaaaaagta
     7141 gctttggaat attaaatcaa ctaaaatcaa aaaataatga aaaaaatagt attcgtatta
     7201 actgcaatta ttgccttttc ttttagctct aatgcccaaa acaaaaaaga gttatccaaa
     7261 gaagacatgg ttaaacaaga acagttaaaa aaagaaagcc ctgaagttaa tgctaaaaat
     7321 gacgctgtgg aattgtctaa atatttaggt ttagatgata ctatgacaaa tgatttttac
     7381 agactttttg aaatgaaata taaaacactt caaaatgaat taactcctga aagaaaaaca
     7441 gaactttcaa gagtaatgga tgccaaaatt agagcaacaa ttggcgatgc taaaatggaa
     7501 aaacttgaat caaatgaagc tttatacaaa aggttaatta attaataatc actattataa
     7561 gtcaataaaa ccattccatt gaatggtttt atttatttat agctgttttg aaacagtatc
     7621 aatcgcttgt attgtaaaat ctaaatcttc ataggttaat gcatccgaga taaaccaagt
     7681 ttcataggct gaaggtgcta tatatactcc ttcttgtaac aatccgtgaa agaatttttt
     7741 aaacctatca ttatcgccat ttttagcaga attaaaatcg aaaaccgggt tttcatcaaa
     7801 atgtacagaa atcatggagc caactctatt tatggtaaat tcgattttat tttgagttaa
     7861 aaccgtttca attcctttgt gtaaataagc cgttttttct tccaatcttt taaaaattgt
     7921 aatatcctta tttaaacttt gcaacattgc atagccagct gccattgcta atggatttcc
     7981 agataaagtt cctgcttgat aaactgaccc aagtggagaa agatgattca taatttcatt
     8041 acgagctgca aaagcaccaa caggcaaacc gccaccaatt acttttccga agcaaactaa
     8101 atcagcagca acattatata gctcttgtgc tccacctttt gcaagtctaa atccagtcat
     8161 cacttcatca aaaatcaata acgaattatt ggcgtcacat accgctctta aattttgaag
     8221 aaagccatca gttggcggaa cacaacccat atttcctgca acgggttcga taattatagc
     8281 cgcaatttca tttttatttg actcaaaaag tgcttttaca ttttcgatgt cgttataatt
     8341 tgctaacaaa gtatcctttg cagttccttg tgtcactcca ggagaatttg gagttccaaa
     8401 ggtacttgct ccacttcctg cttgaattaa aaaagaatct gaatgtccat gatagcaacc
     8461 tgcaaatttt atgattttat cttttttggt aaatcctcta gccaaacgaa tagcactcat
     8521 acaagcttct gtaccagaac ttacaaatct tattttatca atatttggca ccatagaaac
     8581 tgctaatgtg gcaatttttg tttctaattc ggtgggcatt ccaaatgaag ttccaagttt
     8641 tgttttttca attaccgcgt caacaacggg ttgaaaagca tgacctaaaa tcattggtcc
     8701 ccaagaatta atataatcaa tatagcgatt attatcttca tcatagacat aagcaccttt
     8761 agcactcttt gcaaaaattg gtgtacctcc tactccttta aaagctctta caggagagtt
     8821 aacaccacca ggaattacct tttctgcctc agcaaaaagc tgactacttc tttgatataa
     8881 catttgaaat tatttaggaa tgtttaattg acgtgtaatc tttgtcctag tgacaaatta
     8941 gtatccgata aattattttt ctttttcagg tcttctatag atatattaaa ccgttttgaa
     9001 atcgaatata aagtatctcc tttaacaaca gtgtattttg atggatcttg ataagccact
     9061 tgagaattgg gatttgtttc ataatctttc cctagaactt cagcgtcaaa acgctgtaaa
     9121 tcataccgtt caattaaact gattaatttg tctggatatt ttggatctgt tgcataacct
     9181 gctgctttta atccttttgc ccatctttta taatcatctt tatccaattt aaacaaatcg
     9241 gcatatcgtg atctacttgt taaaaaataa gagtgatctc taaaagattc gctaggatca
     9301 ttgtattttc taaaacattc atcttctgca tcatcagtat attttatact tggtccatcc
     9361 cattctttat gacatttaat accaaaatga ttgttggctt gcgcactcaa tggtccggta
     9421 cctgcacccg actctaaaat agcttgccct agagttatac ttgctggaat tccatttttc
     9481 accatgttat ctttggcaat ttctttaaaa gtatcgatgt attctaagac taactttgta
     9541 gtcactttta cactagttgt tgcttctaaa accacggtac taatatcatt attttgagaa
     9601 gatgatgaat tgtttgaacc agatgttgac gtgttattgg gtcttgaagt taccacttta
     9661 gactttgaac tattatgagc tatgggtttt ctaactaccg ttttgcttga tttagaagtt
     9721 ctaaccacag gttttgagga agaacatgaa actaaaaaaa ttagaagaaa accaattaaa
     9781 aacttagtat gcattatatt ttatttgtgt tagattatta ttttttaaaa ctaaattcat
     9841 cccatttata ccttgcaatc caccagtatg tagtatacgt attttagcat tttcaggaaa
     9901 ataattcttt ttaattaagt caaaaacacc aaaaaccatt ttaccagtat aaattggatc
     9961 tagtggaatt tgatattgag tataaaattc gttcataaaa tcgattaact cgtttgtggt
    10021 ttttgcataa cctccaaaat ggtaatcgtt tatcaattcc caattttctt tgtttgcaaa
    10081 tttacgaata tcttcttgca aaaaatcacc ttttaaagca ggaaatccta aaactttttg
    10141 acagtgtttt gaacaattta ttacacctga aatagttccg cctgtcccaa cagcacaaca
    10201 aatgtaatca aaagcatcat cttcgcttgt tactatttct tcacatccct ttatcgccaa
    10261 caaattggtt cctccttctg gaagcaaata aaaatcaccg aattttaatt ttaatgttgc
    10321 tataaaattt tcgtcatttt tatttcgata attatctctt gaaataaaat caaattccat
    10381 tccaaattct tttgcttttg ataaagtggg gttattttct atattattta ctaactcttc
    10441 tcctctaata attccaattg ttttaaaacc atattctttt cctacagctg caaccgccaa
    10501 aatgtgattg gaataggcgc ctccaaatgt gagtaaagtg tttttattta aacgctttac
    10561 ttcctcaata ttatatttta atttcctgaa tttattgccc gaaatataag gatgtagtaa
    10621 atcctctctt tttatagaaa gtagtttatt ttttattatt atagtttgat acatactttg
    10681 atttgcaaat aattactgtt ttttatgatt ttttttattt ttttgtagta taattattgt
    10741 tttttaccga cgaattgtta ataaccttaa atattattaa caaaagttag aaatattaac
    10801 atttctttct atttttgata gtacccaaat ataacaaact taaaaataat tcagtatgaa
    10861 aaaaaaatac tatttacttg ttttcgcttt tatatttata aaaagtaatt tactttattc
    10921 tcaaactaat tgttcgaccg cactgccaat tacaatcaat ggtgcatgtc aaagtggttc
    10981 agttaatgac aatagcgcag atttaccagc acttactggc tgtaccgcaa cttttaaccg
    11041 agagagatgg tacactttta ctgttgttgg tggttctcaa accattacta ttacaggaac
    11101 atccacaagc accaaccgaa atttatacct tcaattaata tctctaactg gaacttgtgc
    11161 ttctggaact ttaactcaaa ttgcttgtgc aaatgctata aacactaata gtgctcaaac
    11221 tgaaaccatt actatcacat taacaaatgg agtatatttc attaaagttc taaatgt
//
LOCUS       DAOOBH010000072        11266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_7350_length_11266_cov_6.282580, whole genome shotgun sequence.
ACCESSION   DAOOBH010000072 DAOOBH010000000
VERSION     DAOOBH010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11266
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7350_length_11266_cov_6.282580"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..1901
                     /locus_tag="PK218_08020"
     CDS             315..1901
                     /locus_tag="PK218_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPW98492.1"
                     /translation="MRYFVLLVSLIVLFSCKKETEKPIPKKVIIVEREKPFEGRVIAI
                     DTTVYISFKNPELEFFYKSFKNETVWQSTVLRKFVLNEFKNAENEGLFSKDYNYYNLL
                     SLEKKINTLNDKQLVDYDLQLTLNFQKYIKDISQGKLNPKEIYNDWDLKSKNTDVSKI
                     LISAFKREANFVAVIDSCKPQHEIYKKLKTALLIVNSFPKDTLKTILVEEKIVPKDTL
                     KAMIAIKKRLIYWKDLKASDSLTSIYDSKTVEAIKNFQFRHGLASDGVIGKGTLQALN
                     FTKTKRKHQIMANMERWKWYPKEFSKEYLMVNIPDYNLVAVENKDTIRMHKVIVGTNK
                     RKTPILTSKLSYAVFNPTWTVPPTILKEDVIPAMLKNKNYLTKKNITAYDNQGNIVSA
                     EDWTASKAKSYRYVQSPGSYNSLGMVKIIFPNKFSVYLHDTNHRDFFGKTNRSLSSGC
                     VRVENPLELTNYLLKDSINYPMDSITSILKKMETKTAPIKSSIYLYQWYWTAWSNNNK
                     LIFRDDIYNLDEDLYKKLEE"
     gene            complement(1903..2595)
                     /locus_tag="PK218_08025"
     CDS             complement(1903..2595)
                     /locus_tag="PK218_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HPW98493.1"
                     /translation="MKYKIFSLLAFLLLTSFSSLPSKHVDLNSILIKVEKPLSKVESV
                     YFKLKSNSFSMPSFDCFSVAIEGFYKFKEKGIFQKNILTLIDFSQSSNSKRLWIIDLD
                     KDEIILNTYVAHGRNTGDEFANHFSNRAESYQSSLGFYATAEIYNGKHGASLKLDGLQ
                     KGLNDNARERAVVMHGADYVSEKFIHNNKRLGRSLGCPAVPVELNDKIISLIKNKSCL
                     FIYHSSQKDVFI"
     gene            2786..5221
                     /locus_tag="PK218_08030"
     CDS             2786..5221
                     /locus_tag="PK218_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPW98494.1"
                     /translation="MPKLYNLFILVIFLHSNIFLGQKKTLQSIRTTEKITIDGLLDEA
                     IWKIAPIATDFVMINPGNGTPIPNRQKTDVKIVYDNEAVYVAATLYDDEPSKILKELT
                     LRDDFATADHFGVFFNGYNDGQHEFRFFVSAAGVQQDGLYTDALGEDFSWDAIWKSEV
                     KITDFGWVVEMKIPYAALRFPNSAVQTWGLNFYREVRRDRYQYCWNLISNTIQNEATQ
                     AGVLEGIENIKTPTRLFFIPYSSFYLNTKKGESSVNEFKAGMDIKYGINDAFTLDAIL
                     VPDFGQTKFDNVELNLSAFEQIFAEQRPFFTEGTDLFSKGDLFYSRRIGSEPSTYPDT
                     TSDEEVINYPNTVNLLNALKISGRTKSGLGIGVLNAITEKANSIVRKTTINPDNSETI
                     SERDVVVEPLANYNVLVLDQRFNKNSSVSLVNTNVTRNGEYRDANVSALVWDLNTKKN
                     TFNLDGDFKYSYVNELSDLEDRKGYVSSLSFGKTSGKRRFRIGGNYVSNEFDNNDLGV
                     NFQTHYHSLYGNTSYRILKPNELFNEFSTYLNLYSEYDNRTGRLQAGSANLQFNITNK
                     KNDYISFAVNTVPLKTYDFYEPRSFDERRFVEIPEQIHFSFLFSGNYNRRFALDFNPS
                     FTFVNELERVNYVFSIGPRYRFNDKFSINANFRFVRQNKNTGWAAFDEFDNTIFARRD
                     RKSITSTLSAKYSINNKMNFNLNVRHYWSSLNVLEYLTLLDNGTFEKNTTYSENKNQN
                     LDTWNLDLAYSWWFAPGSQISILYRNNSYLFTRNNSQTFSENLNESLDSKNLNHVFSI
                     SIRYFIDYNSLKK"
     gene            5313..6830
                     /gene="gpmI"
                     /locus_tag="PK218_08035"
     CDS             5313..6830
                     /gene="gpmI"
                     /locus_tag="PK218_08035"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPW98495.1"
                     /translation="MNKKVILMILDGWGKSPDPKVSAIDNANTPYIDSLYTKYPNAQL
                     RTDGLNVGLPEGQMGNSEVGHMNLGAGRIVYQDLAKINLAVANKTMSIEKPLLDAFHY
                     AKENNKPVHFLGLVSDGGVHSHTSHLRGLIDASQDFGLEKVYVHAFTDGRDVDPKSGI
                     KYIEDLQNYISNTTVKIASVIGRYYAMDRDKRWERIKLAYDLIVNGFGKKALNPILAM
                     KESYENNITDEFVEPVAIVDENNDPIAKIQNDDVVIFFNFRTDRGRELTEALSQKDFH
                     EQNMHKLNLYYVTMTNYDDTYQNVHVIYDKDNLTETLGEVIEKAGKTQIRIAETEKYP
                     HVTFFFSGGREIPFVGETRILRNSPKVATYDLQPEMSAFELTAALVPELQKGSVDFVC
                     LNFANGDMVGHTGDMQAAIKACEAVDVCVKKVTETALENDYTTIIIADHGNCETMINP
                     DGSPNTAHTTNPVPIIMVDNQKINVHNGVLGDIAPTILELMGIEKPTVMTGKSLL"
     gene            6861..8282
                     /locus_tag="PK218_08040"
     CDS             6861..8282
                     /locus_tag="PK218_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98496.1"
                     /translation="MKNIFALIILTFIGHVGNAQIVTIPDANFKAKLLAANPNNTIAS
                     NNFANVTIDTNGNNEIEVNEALQITTLNINISGIANLTGIEAFTNLRSLNCTQNQISS
                     LNLSSLTNLKSLYCNLNLLTSLDVSSLSSLTTLECAENQLTNINFGAITGLQNLSCRF
                     NFLTSLEVTQMTNLSSLNCDYNQLTSLNLKNLSLLTDLACQSNQLTFLDLNGLNNLEL
                     VNCSFNQLPTIDLSGLELLTTLNCSHNQLSVLHLEDLSNLHLLNCESNQINSTLNLNG
                     LSNLNGLYCRNNQISNIDFSEPINLEIIDCGFNQFVAIDASPLTQLSVLYCQNNSQLT
                     NLNLKNGSSEQFLDFSECENMTTICADEGDLIAIQNLISEYGYANCQVSSTCDLANVS
                     NDTNSYFSLSPNPVKHLIQLQTNDTVAIKSINVYDILGQIVQFIPNAEGISTIDVATL
                     KTGTYFIKVTTDKGISTVKFLKE"
     gene            complement(8313..9581)
                     /locus_tag="PK218_08045"
     CDS             complement(8313..9581)
                     /locus_tag="PK218_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPW98497.1"
                     /translation="MSKYVTAQEAVKVVKSNDRVYLQAAAAAPTVLANALTERASELK
                     NVEICHLHTEGEARYANPELRESFHVNSFFIGANVRHTIKAGNGSYTPVFLSELPNLF
                     RKNVLPLDVAFIHVSPPDSHGYCSLGVSVEATVAAIENAKTVIAQVNPQMPRTFGDGI
                     LHVSEIDYLVEVNVPIFSHEITPFSKEEEKIGEYVASLIEDRSTLQMGIGSIPNAALS
                     KLIHHKDLGLHTEMFSDGVIDLIENDVINCNYKGTLRGRALATFLIGSKRLYDFVNDN
                     PFIEMKESSMVNDTARIRKNPKMVAINSAIEVDVTGQVCADSIGAKMYSGVGGQMDFI
                     RGAALSHGGKAIIALPSITKKGESRIVPFLKQGAGVVTTRSHAQYIITEHGIADLYGK
                     TLKQRMDSLIRIAHPMHQERIEKEYYEMLK"
     gene            9722..10396
                     /locus_tag="PK218_08050"
     CDS             9722..10396
                     /locus_tag="PK218_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW98498.1"
                     /translation="MNTVLTHIKIQVNEKIYVKDPETSTLGKKIIQESILLIEAIGFE
                     DFTFKKLGERIGSNESSIYRYFESKHKLLLYLSSWYWGWMEYRLVFSTNNIKSPLEKL
                     KKAITIVTEKIEDDSLTLHINESILNKIIISEFNKTFLTKEVDQENKEGYFLVYKRVI
                     NRIVEMIEEVNPKYKFTKSFASSIVEGAIHQHFLKDHLTTITNCNQNTSVTDFYIDLI
                     EKTLNP"
     gene            10399..>11266
                     /locus_tag="PK218_08055"
     CDS             10399..>11266
                     /locus_tag="PK218_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW98499.1"
                     /translation="MALPSSKRLLNLLKLDKKDISQIFFYAIFSGLVSLSLPLGIQAI
                     INFIQSGQVSISWVVLVFVVIVGVVLVGILSLMQLRITENLQQKLFVRSSFEFSYRLP
                     KIEFEELYNKYAPELANRFFDTLTIQKGVSKILIDFSSALLQITFGIILLSLYHPFFI
                     FFGMFLFILLYVIFKFSYNTGLSTSLKESNYKYKVVSWLQEIARNNFSFRKNINFDYA
                     LTKNDLLVSEYLNYREKHFGIIKRQYIQLIAFKIIITAGLLLIGGYLVLNQQMNIGQF
                     VAAEIIILLVINS"
BASE COUNT         4000 a         1795 c         1749 g         3722 t
ORIGIN      
        1 aactcgcgac aaccagcttg gaaggctgga gctctaccaa ctgagctact tccgcttttg
       61 tgagtgcaaa tatatagcat ttgttttatt cgatgcaact tttttttaaa aatatttttt
      121 gaagaattta atttgtgttt ttcataagtt ttaattgttt tactattaaa aagttatatt
      181 tttatagcac ttatcataat gtgcttttac tataaaatct tcaagaaaaa gattaaaatt
      241 taaaaaatag ccttattttt aattataaga tgaagctaaa ttgcattgtg tctgtttctt
      301 ttataaattg taatatgagg tattttgttt tattagtcag tctaattgtt ttgttctctt
      361 gcaagaaaga aacagaaaaa ccgattccta aaaaagtgat tattgttgaa agagaaaaac
      421 cttttgaagg aagagtaatt gccattgaca ctactgtata tatatctttt aaaaatccag
      481 aattagagtt tttttacaaa agctttaaga atgaaaccgt ttggcaatca accgtgttgc
      541 gtaaatttgt tttaaatgaa tttaaaaatg cagaaaatga agggttgttt tctaaagatt
      601 ataattatta taacctattg agtttggaaa aaaaaatcaa cacattaaac gataaacaat
      661 tggttgatta tgatttgcaa ctaactttaa attttcaaaa atacattaaa gatataagtc
      721 aaggaaagtt aaacccaaaa gaaatttata acgattggga tttaaaatca aagaatacgg
      781 atgtctcaaa aattcttatt agtgcattta aaagagaagc taattttgtg gcagtaattg
      841 atagctgcaa acctcaacat gaaatatata aaaagctaaa aacggcactt ttgattgtaa
      901 actctttccc aaaagacact ttaaaaacta ttcttgttga agagaaaatt gttcctaaag
      961 acactctgaa agcgatgatt gctattaaaa aaagattgat ttactggaag gatttaaaag
     1021 ccagtgatag tctaacttct atttacgatt caaaaacagt ggaagctatt aagaattttc
     1081 aatttcgaca tgggttggct tcggatggtg taattggtaa aggaacgtta caagctttaa
     1141 attttacaaa aaccaaaaga aaacatcaaa tcatggcaaa catggaacgc tggaaatggt
     1201 atccaaaaga gttttccaaa gaatatttga tggtcaatat tccagattat aatttggtag
     1261 cagttgaaaa taaagacact atccgaatgc acaaagtaat tgttggcact aacaaaagaa
     1321 aaacacctat tttaacttca aaattaagtt atgccgtatt caatccaact tggactgttc
     1381 cgccaacaat tctgaaggaa gatgtgattc ctgcaatgct taagaacaaa aattatttga
     1441 ccaaaaaaaa cattacagct tatgataatc aaggaaatat tgtttcagca gaagattgga
     1501 cagcttcaaa agctaaaagt tatcgctatg tgcaaagtcc gggcagttac aattcgcttg
     1561 gaatggtaaa aattattttt ccaaataaat tttcggttta tcttcacgac acaaatcatc
     1621 gtgatttttt tggaaaaacc aatcgctcct taagttcagg ttgtgtcagg gttgaaaatc
     1681 cgcttgaatt aaccaattat ttattaaaag attccattaa ttatccaatg gacagcatta
     1741 cttctatttt gaaaaaaatg gaaaccaaaa cagcacctat aaaatcgtca atttatttat
     1801 atcaatggta ttggacagct tggagcaaca ataacaagct gatttttaga gatgacattt
     1861 acaatctaga tgaagactta tataaaaaac ttgaagaata atctagataa atacatcttt
     1921 ttgagaagaa tgataaataa acaaacatga tttatttttt atcaaactga taattttatc
     1981 atttaactct accggaacgg caggacaacc aagacttctc cctagtcttt tattgttgtg
     2041 gataaatttt tctgaaacat aatcagcacc gtgcataaca actgctcttt ctctagcatt
     2101 gtcattcagt cctttttgca aaccatctaa ttttaaggaa gcaccatgtt ttccgttata
     2161 aatttcggca gtagcataaa aacctaaact actttgatag gattccgctc tatttgaaaa
     2221 atgattggca aattcatcgc ctgtatttct accatgagca acatatgtat ttaatattat
     2281 ttcatcttta tccaaatcaa ttatccaaag ccgttttgaa tttgaagatt gcgaaaaatc
     2341 aattaaagtt aaaatgtttt tttgaaaaat acctttttct ttaaatttat aaaatccttc
     2401 aatggcaact gaaaaacagt caaaactagg cattgaaaaa gagtttgatt ttaatttaaa
     2461 ataaaccgac tctacttttg ataaaggttt ttctactttt atcaatatgc tatttaagtc
     2521 aacatgtttc gatggcaaac tgctaaaaga agttaataac agaaacgcta atagggaaaa
     2581 aatcttatat ttcattgatt gtgataggtt taaatgttag acttgggatg gcaaatctat
     2641 aaaaaaaaat taaacttcaa aatttatctt cacttaagat tgggttaatt aattatcgat
     2701 taagtgaaaa agtagcttat ttattgcact tactcttgtt tatgtcaata aattttctat
     2761 ttttgctata catttaagtg aataaatgcc caaattatat aacttattta ttctcgttat
     2821 ttttttacac tcaaatatat ttttgggtca aaaaaaaacg cttcaatcca taagaactac
     2881 cgaaaaaatt acaattgacg gtttattgga tgaagcaatt tggaaaattg ctcccatagc
     2941 aactgacttc gtgatgataa acccaggaaa cggtactcca attcctaatc gtcaaaaaac
     3001 tgatgttaaa atagtatatg acaacgaagc ggtttatgta gcggctacac tttatgatga
     3061 tgaaccttct aaaattttaa aagaattaac attaagagat gattttgcta ctgctgatca
     3121 ttttggtgtt ttctttaacg gatataatga tggtcaacat gaatttcgct tttttgtaag
     3181 tgcagcaggt gttcaacaag atggtttata tactgatgct ttgggagaag attttagttg
     3241 ggatgcgatt tggaaaagcg aagtaaaaat caccgatttt ggttgggttg ttgaaatgaa
     3301 aattccttat gctgctttac gctttcctaa ttcagctgtt caaacttggg gtttaaattt
     3361 ttatcgcgaa gtacgccgtg acagatacca atactgctgg aatttaatta gtaacaccat
     3421 tcaaaatgaa gctactcaag ccggtgtttt agaaggcatt gaaaacatta aaactcctac
     3481 acgtcttttt tttataccct attcgtcctt ctatttaaac acaaaaaaag gagaaagcag
     3541 cgtaaacgaa tttaaagctg gaatggacat aaaatatggt attaatgatg cattcacttt
     3601 agatgctata ttagttccgg attttggtca aactaaattt gataatgttg agctaaatct
     3661 gagtgctttt gaacaaattt ttgcagaaca aagaccattt tttacagaag gcaccgattt
     3721 gttttcaaaa ggcgatttat tttactctag aagaattgga agcgaacctt caacctatcc
     3781 cgacaccaca tcagatgaag aagtaatcaa ttatccaaac actgtaaatt tattaaatgc
     3841 attaaaaatt tctggaagaa caaaaagtgg tttaggaatt ggcgttttaa atgctataac
     3901 agagaaagct aattcaatcg tccgaaaaac cacaataaat cctgataata gcgaaacgat
     3961 ttctgaaaga gatgttgttg tagaaccttt agctaattat aatgtattag tcttagacca
     4021 gcgatttaac aaaaactctt ccgtttcttt agtcaacacc aatgttacac gcaatggtga
     4081 atatagagat gccaacgtat cagcattagt atgggattta aatactaaaa agaatacctt
     4141 taatttagac ggtgatttta aatacagcta tgtaaatgaa ctttcagatt tagaggatcg
     4201 aaaaggttat gtgtcatctt tgagttttgg aaaaacaagc gggaaaagac gttttagaat
     4261 tggtggaaat tatgtttcaa acgaatttga caacaatgat ttgggtgtaa atttccaaac
     4321 tcattaccat tctttatacg gaaacaccag ctatcgtatt ctaaaaccca atgaactctt
     4381 taatgagttt agcacctatt taaatttgta ttcagaatac gataacagaa ctggaagact
     4441 acaagctgga tccgcaaatt tgcaatttaa cattacgaat aaaaaaaatg attatataag
     4501 ttttgctgta aacacggttc cattaaaaac atatgatttt tatgaaccac gctcttttga
     4561 cgaaagaagg tttgttgaaa ttcctgaaca aatacatttt tcttttttgt tttcaggaaa
     4621 ttataatcga agatttgctt tagattttaa cccatctttc acttttgtaa atgaactaga
     4681 aagagtaaat tatgttttct caattggccc aagataccga tttaatgata aattttctat
     4741 aaacgccaat ttcagatttg tgcgacaaaa taaaaacaca ggttgggcag cttttgatga
     4801 atttgacaac accatttttg ctcgaagaga tcgaaaaagc atcaccagca cactaagtgc
     4861 aaaatattct ataaataaca aaatgaactt caatttgaat gttagacatt attggtcgtc
     4921 attaaatgtt ttagaatatt tgactctttt agacaatgga acttttgaaa aaaacactac
     4981 ttattccgaa aataaaaatc aaaacttaga cacttggaat ttggatttgg cttattcttg
     5041 gtggtttgct cctgggagtc agatttctat tttataccga aacaattcct atttatttac
     5101 ccgaaacaat agccaaactt tcagtgaaaa tttaaacgaa tcattagaca gcaaaaacct
     5161 aaaccatgtt ttttcaatca gtattcgtta tttcatcgat tataattcgt tgaaaaaata
     5221 actgtttatc cttcaaattt ttatcaaatt cgcatcatta aaaaatagtg cgctttttta
     5281 aaatgaatta tctttgttca aaataatttc tgatgaacaa aaaagtaatt ctaatgattt
     5341 tggacggttg gggaaaatct cccgatccaa aagtttctgc cattgataat gctaatacac
     5401 catatataga cagtttatac acaaaatacc caaatgctca attgagaacc gatggcttaa
     5461 atgtaggttt acccgaaggt caaatgggaa attctgaagt aggacacatg aatcttggtg
     5521 ctggaagaat tgtttatcaa gatttggcaa aaattaattt ggcggtcgcg aacaaaacta
     5581 tgagtataga aaaacctttg ctagatgctt ttcattatgc caaagaaaac aacaagccgg
     5641 ttcatttttt aggattagtt tcggatggcg gcgtacattc tcacacatcg catcttcgtg
     5701 gattaattga tgcatcacaa gattttggtt tagaaaaagt atatgttcac gcttttacag
     5761 atggtcgtga tgttgaccca aaatcaggca ttaaatatat tgaagattta caaaattata
     5821 tttcaaatac tacagtaaaa attgcttcag taattggtcg ctattatgca atggatagag
     5881 acaaacgctg ggaaagaata aaattggcat atgatttaat tgtaaatggt tttggcaaaa
     5941 aagcattaaa tccaatcctt gcaatgaaag aaagctatga aaacaacatc acagatgaat
     6001 ttgtagaacc tgttgccatt gttgatgaaa acaatgaccc aattgcaaaa attcaaaatg
     6061 atgatgtggt gattttcttt aactttagaa ccgatagagg aagagaattg accgaagcac
     6121 tttcacaaaa agattttcac gaacaaaaca tgcacaaatt gaatttgtat tatgttacga
     6181 tgaccaatta tgacgatact tatcaaaacg tacatgtaat ttatgataaa gacaatttaa
     6241 cagaaacact tggtgaggtt atagaaaaag ccggaaaaac gcaaatcaga attgccgaaa
     6301 cagaaaaata cccacacgtt acttttttct tttctggagg aagagaaatt ccatttgtag
     6361 gcgaaacccg tattttaaga aactctccaa aagtagccac gtatgactta caacccgaaa
     6421 tgagtgcttt tgaacttaca gcagcattag ttcccgaact tcaaaaaggc tcggtagatt
     6481 ttgtttgtct taactttgcc aacggcgaca tggttggaca tactggcgac atgcaagcgg
     6541 caatcaaagc atgtgaagct gtagatgttt gtgtaaaaaa agtgactgaa acagctttgg
     6601 aaaatgatta tacaactatc attattgccg atcatggaaa ttgtgaaaca atgattaatc
     6661 cagatggttc gccaaacacc gcacacacta ccaaccccgt tccgataatt atggttgata
     6721 atcaaaagat taatgttcac aatggtgttt tgggtgatat tgctcctact atattggaac
     6781 taatgggaat tgaaaaacca acagtaatga ctggtaaatc attgctttaa taaaaattct
     6841 atctgaaatt caatcatttt atgaaaaata tctttgcact tataatctta acattcattg
     6901 gacatgttgg taatgcacaa attgtaacta ttcccgatgc taattttaag gcaaaattat
     6961 tggctgcgaa tccgaacaat accattgctt ctaacaactt cgccaatgta accattgaca
     7021 ctaacggcaa caacgaaatt gaagttaacg aagctctaca aattacaact ttaaacataa
     7081 acatttcggg aatagctaat ttaactggaa tagaagcttt caccaatctt agatctttaa
     7141 attgcactca aaatcaaatt tcatcgctaa atctaagtag tttaaccaac ttaaaatcgt
     7201 tatactgtaa tttgaattta ttaacttcat tagacgttag tagcttatct agtcttacaa
     7261 ccctagaatg tgcagaaaat caattaacca atattaattt tggagccatc actggtttgc
     7321 aaaacttgag ttgtagattt aattttctaa cttcattaga agtaactcaa atgacaaatc
     7381 tatcgtcatt aaattgtgat tacaaccaac ttacttcatt aaacctcaaa aacttatcac
     7441 tattaacgga tcttgcttgc caatccaatc aacttacttt tttagattta aacgggttga
     7501 ataatttaga attagtaaac tgttcgttca atcaactacc aaccatagat ttaagtggtc
     7561 tagaattatt aaccacttta aactgctctc acaaccaact ttctgttcta catttagagg
     7621 atttatccaa tttacatcta ttaaattgtg agtcaaacca aattaattct actttaaatt
     7681 taaacggatt atcaaacttg aatgggcttt attgtcgtaa taatcaaatt tcaaatattg
     7741 attttagcga acctattaat ttagaaataa ttgattgtgg tttcaatcaa tttgttgcta
     7801 tcgatgcaag tccattgaca caactttcgg ttttatattg tcaaaacaat tcgcaattga
     7861 ctaatttgaa tctcaaaaac ggaagttcag aacagttttt agatttttcg gaatgcgaaa
     7921 acatgactac tatttgtgca gatgaaggag atttaattgc gattcaaaat cttatttcgg
     7981 agtatggata cgccaattgc caagtcagtt caacctgtga tttagcaaac gtttctaatg
     8041 acacaaactc ttattttagt ttatcaccta atcctgtgaa gcatctcata cagttacaga
     8101 ctaatgatac agtagcaatt aagtcaataa atgtatatga tattttggga caaatagtgc
     8161 aatttattcc aaatgctgaa ggtatttcaa ctatcgatgt ggccacctta aaaactggaa
     8221 cttattttat aaaagttact accgacaaag ggatttcaac tgtcaagttt ttgaaagagt
     8281 aacagtaaaa tcgctgtgtt tagatcctca ttttatttca gcatttcata atattccttt
     8341 tcaatgcgtt cttgatgcat tggatgcgca attcgaatca aggaatccat acgttgtttt
     8401 aaagtctttc catataaatc ggcaatgcca tgttcggtga tgatatattg agcgtgagaa
     8461 cgtgtcgtta caacacctgc accttgcttt aaaaaaggca caatacggct ctctcctttt
     8521 ttagtaatcg acggcaatgc tattatggct tttccaccat ggcttaaagc tgcacctcga
     8581 ataaaatcca tttgtccacc aacacccgaa tacattttgg cgccaataga atcggcacaa
     8641 acttgtcccg ttacatctac ttcaatagcc gaattaatcg ctaccatttt tgggtttttt
     8701 ctaatacgtg ccgtgtcatt taccatcgat gattctttca tttcgataaa tggattgtcg
     8761 tttacaaaat catacagtct tttagatcca attaaaaaag tagccaaagc tctaccgcga
     8821 agtgtccctt tgtaattgca attaatcaca tcattttcta ttaaatcaat tactccatcc
     8881 gaaaacattt cggtatgtaa tcctaaatcc ttatgatgta ttaatttact caatgcagca
     8941 tttggaatgg agcctatacc catttgcaac gtgcttctat cttcaattaa tgacgctaca
     9001 tattcgccaa ttttttcttc ttctttagaa aaaggtgtaa tttcatgaga aaaaataggc
     9061 acattcactt caaccaaata atcaatttct gaaacatgta aaataccatc gccaaacgtt
     9121 ctaggcattt gcggattgac ttgtgcaatg actgttttgg cattttcaat agcagccaca
     9181 gtggcttcaa cagaaacgcc aagcgaacaa tatccgtgac tatctggcgg cgaaacatga
     9241 ataaaagcca catcaagcgg taacacgttt tttcgaaaaa gattgggcaa ttcgcttaaa
     9301 aaaacaggcg tataagagcc atttccagct ttaattgtat gacgaacatt ggcaccaata
     9361 aaaaaagagt tgacatgaaa actctctctc aactcaggat ttgcataacg tgcttcgcct
     9421 tcggtatgaa gatgacaaat ttctacattc tttaattccg aagctctttc ggtcaaagca
     9481 tttgctaaaa cagttggtgc tgcagcggca gcttgaagat aaactcgatc attagatttt
     9541 actactttca cagcttcttg ggcggtaaca tacttgctca tgattttttt tgcaaaatta
     9601 ggaggattat agattttgct aaatgatttt tctcatgttt tttttaaata aatcattaat
     9661 taaaagttaa aatttcgttt ttgatagtaa tactattata tttgcatgaa ataatattaa
     9721 aatgaacacg gttttaacac atatcaaaat tcaagtaaat gaaaaaattt atgtaaaaga
     9781 ccctgaaacc tctactcttg gaaaaaaaat aattcaagaa agcatactct taattgaagc
     9841 tataggtttt gaagatttta catttaagaa acttggagaa cgaattggct ccaacgaaag
     9901 ttcgatttat aggtattttg aaagtaaaca taaactttta ttataccttt cttcatggta
     9961 ttggggttgg atggaatatc gattggtttt ttcaaccaat aacattaaga gtcctctaga
    10021 aaaactaaaa aaagccataa ctattgtgac agaaaaaatt gaggatgatt ctttaacact
    10081 tcatattaat gaatccattt tgaataaaat tattatttcc gaattcaaca aaaccttttt
    10141 aactaaagaa gttgaccaag aaaataaaga aggctacttt ttggtttata aaagagtcat
    10201 caatagaata gtagaaatga ttgaagaagt gaatccgaaa tataaattta cgaaaagttt
    10261 tgcatcatca attgtagaag gagcaataca ccaacatttt ttaaaagacc atttgacaac
    10321 cattaccaat tgcaatcaaa acactagcgt aaccgatttt tatattgact taattgaaaa
    10381 aacattaaac ccataattat ggctttacct tcctctaaga gattacttaa cttattaaaa
    10441 ctagataaaa aagatatttc tcaaattttt ttctatgcta tattttctgg tttggtcagc
    10501 ttgtcattgc cattaggaat tcaggcaatc atcaatttca ttcaatctgg acaagtaagt
    10561 atatcatggg ttgtattggt atttgtagta atcgttggtg tagttttagt tggtatttta
    10621 tctttaatgc aacttagaat tactgaaaat ttacaacaaa aactctttgt gcgttcttca
    10681 tttgaattta gttatcgatt acccaaaatt gaatttgaag aactttacaa caaatatgca
    10741 cctgaattag ccaatcgctt ttttgatact ttaaccattc aaaaaggagt ttcaaaaata
    10801 ttaattgatt tttcctccgc tttacttcaa ataacttttg gaattatact gctatccctt
    10861 tatcacccat tttttatttt cttcggaatg tttcttttta ttttactgta tgtgatcttc
    10921 aaattttcat acaacaccgg actttctact agtctaaaag aatcaaatta caaatataaa
    10981 gtggtaagtt ggcttcaaga aattgcccga aacaacttca gttttcgaaa aaatataaat
    11041 tttgattatg ccctaacaaa aaatgattta ttggtttccg aatatttaaa ttatagagaa
    11101 aaacattttg gaataataaa aagacaatat atacagctta ttgcttttaa aattatcatt
    11161 actgccggat tacttttaat tggaggctat ttggttttaa atcagcaaat gaacataggt
    11221 caattcgttg cggcagaaat catcatcttg ttggttatta actcgg
//
LOCUS       DAOOBH010000073        11129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_7454_length_11129_cov_5.887484, whole genome shotgun sequence.
ACCESSION   DAOOBH010000073 DAOOBH010000000
VERSION     DAOOBH010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7454_length_11129_cov_5.887484"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1673
                     /locus_tag="PK218_08060"
     CDS             60..1673
                     /locus_tag="PK218_08060"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPW98500.1"
                     /translation="MNQTKYIFVTGGVTSSLGKGIIAASLAKLLQARGYRTTIQKFDP
                     YINVDPGTLNPYEHGECFVTDDGAETDLDLGHYERFLNVPTSQANNVTTGRVYLSVIE
                     KERRGEFLGKTVQVVPHITNEIKDRMQLLGKSGNFDIVITEIGGTVGDIESLPYIESV
                     RQLVWELGENNGIVIHLTLVPFLAAAGELKTKPTQHSVKTLMESGIKADILVCRTEYE
                     LSEELRQKLALFCNVKREAVIQSIDASTIYEVPNLMLEEGLDVVALKKLSLSEQKIPD
                     LTNWNTFLDRLKNPKYTVNVGLVGKYVELQDSYKSILEAFIHAGAANETKVNVISIHS
                     EYLDANSADKQLKNLDGILVAPGFGERGIEGKIETVRYARENNIPFFGICLGMQMAVI
                     EFARNVVGLKEANSTEMNENTPNPVISIMEEQKNITDKGGTMRLGSWKCDLDNNSLAH
                     SIYNKLQIEERHRHRYEFNNKYRSQLEENGLKASGVNPDTGLVEIIELENHPFFIGVQ
                     YHPEYKSTVANPHPIFVSFVAAMKKHKNN"
     gene            1701..3596
                     /gene="yidC"
                     /locus_tag="PK218_08065"
     CDS             1701..3596
                     /gene="yidC"
                     /locus_tag="PK218_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPW98501.1"
                     /translation="MEQKNKFDLNSLIGFALIFVIIIWIMTNSKNSEEQKQLDKVKQE
                     QVDKEKKTVPAQTIAETLKDSTVTDTAKVEKLKSVLGSFAYSASLPSAKDSITTIENE
                     LLVLKVANKGGYISEVTLKDYKRFDKNSKELVSLIKNNNSNFNLELKTNDNRILNSKD
                     LYFQPTLTKNGESQILSMKLKAGPNEFLEYKYVLNNKNYMLDFEIQTQGLAKVLNTSK
                     PSNLEWDLKAYRNEKSISYENRYAELYFEHKNGKVDYAGLGSQEDKDLENATFIAFKQ
                     HFFSSILLTKTPIEGASIHSTNLVHDEDKDTIFTKQFKTILPLAYKNGELDYKMNWYF
                     GPTDYKILNSYDRNIEHIVQLGWGIFGWINKFIFVPLFGFLSNFMSLGITIIVFTILI
                     KLAMSPITFKSFLSQAKMKVLRPEITELGEKFKKDPMKKQQETMKLYNKAGVNPMAGC
                     IPALIQIPFVYASFQFFPSAIQLRQQSFLWADDLSSFDQIFKLPFRIPMYGDHVSLFP
                     ILAAIAIFFYMKMTSGDQAMAQPQQEGMPDMSKIMKVMIYISPLMMLIFFNSYGSGLS
                     LYNFMSNLITIGIMLVIKKYFIDSDKIHAQIQENKTKPKKESKFQKKMREMMEQAEAQ
                     KQLKNKK"
     gene            complement(4503..5447)
                     /locus_tag="PK218_08070"
     CDS             complement(4503..5447)
                     /locus_tag="PK218_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPW98502.1"
                     /translation="MKNLFKSLMVFAIAITLPSCSNDDNNSQPKTIAEIASANGDLST
                     LVEALQRTNLVNTFNSSGDFTVFAPTNAAFATFLATTPYATINDVPVPILTEILKNHV
                     LSESFTSSELMTGYYKTLAKETASSTNTLSMYIDLTAGVKINGGVSNGGANVILTSAN
                     ILASNGVIHVVDRVIGLPTIKNHAIANPNLSIVTGLLVDQGLVPTLDGTTGSPFTVFA
                     PINSAFDTATLNLYTGLSDANKTAVLLYHVVGNANVLSNAIPTGAITTLQGQSFSISG
                     TSIDDSGSSVNKNIVLTDVQCSNGIVHAIDKVLLPNLN"
     gene            5737..6444
                     /locus_tag="PK218_08075"
     CDS             5737..6444
                     /locus_tag="PK218_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98503.1"
                     /translation="MKKILLLYILSTSILYSQDINKLDNNGKKNGLWKGVHEESQRPR
                     YEGTFNHGKEVGVFKFFDDTKAGTVIATREFNPKDDSCYTIFYNQKNNKVSEGKVINK
                     QFEGEWKYYHEDLPIIMTLEFYVKGKLNGVRKVFFKSGELAEETNYIDGVKNGTSKIY
                     LENGVVIEDTNYKNGEYDGKAIFRTSDNQISAEGVFKNGKKVGIWKILEKGTLKNVNM
                     NLQGKKFQKRVKPLEEQ"
     gene            6484..7671
                     /gene="mnmA"
                     /locus_tag="PK218_08080"
     CDS             6484..7671
                     /gene="mnmA"
                     /locus_tag="PK218_08080"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPW98504.1"
                     /translation="MKRVVVGLSGGVDSSVAAYLLKEQGYEVIGLFMKNWHDDSVTIS
                     NECPWLEDSNDALLVAEKLGIPFQTVDLSEQYKEKIVDYMFNEYEKGRTPNPDVLCNR
                     EIKFDVFMKIALSLGADFVATGHYCRKGEIKVDGKPVFQLLAGKDENKDQSYFLCQLS
                     QNQLTKALFPIGELTKPQVREIAGKLDLITAEKRDSQGLCFIGKVRLPEFLQQQLQPK
                     EGFIYEIDAQSEIYSKAIPEFKSLEEQLIFESTSITYLPENGKVVGKHQGAHYFTIGQ
                     RKGLNVGGTKEALFIIATDVDSNVIYTGQGHNHPGLFKKTLKVQPSEVHWIREDLKLK
                     NGESLNVKARIRYRQELQNATLYQFESGLYVSFENPQSAITEGQFVAWNIDDELVGSG
                     VIS"
     gene            7697..9307
                     /locus_tag="PK218_08085"
     CDS             7697..9307
                     /locus_tag="PK218_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPW98505.1"
                     /translation="MKKILVSFLLVSFLNAFSQEDAWVYFNDKPNAQTFLDNPLSMLS
                     QRALDRRENQGITLTVQDTPIYEPYVDQISNADGITVMAKSKWLNCVHVRGELTNIQS
                     LTNFSFVNHIRFADNSLNNRFSNPNPIQVINKQLDVQSNFNYGNSTNQIQMLNAQILH
                     QQDYTGQGKIIAVLDSGFINVNSTAPFQRLFDNNLILGGYNFVSHNSDVYSLHNHGTS
                     TLSCMGAFVDGQLVGTAPDASYYLFVTEDVAAENPVEESYWVQAAEESDRLGADVIST
                     SLGYFEYDNPNYSYQYSDLTGDHAFISQGANIAFSKGMIVVASAGNSGATNNPNIGVP
                     AEATNVLAVGAVQANETYALFSSIGPSFDGRIKPDVMAQGQQSTVANTAGQITSANGT
                     SFSCPIMAGAITSFWSAIPWATNQQVLDFVKQSADRFLSPDDQFGYGIPDFQIALAMA
                     QLSTNESLIKKFTLYPNPTNDKISINFPNTIDEINFILYNNLGQKIIEKNIKNEFPQI
                     SLENNSSGIYFYTIQSKSDFQSGKIIKN"
     gene            9318..10262
                     /locus_tag="PK218_08090"
     CDS             9318..10262
                     /locus_tag="PK218_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPW98506.1"
                     /translation="MNKITQLFNIKYPIIQGGMIWNSGYKLASAVSNAGGLGLIGAGS
                     MYPEVLREHIQKCKKATNKPFGVNVPMLYPNIEEIIQIIIEEGVKIVFTSAGNPKTWT
                     SHLKENGITVVHVVSSSKFALKAQEAGVDAVVAEGFEAGGHNGREETTTLTLIPMVKE
                     KIQIPLIAAGGIATGRGMLATMILGADGVQMGSRFAASIESSAHENFKKTITETGEGD
                     TQLTLKELAPVRLIKNKFYNDVQELYSTCPSKDALEQLLGKRRAKRGMFEGDLLEGEL
                     EIGQIAGIIHDISTVEQIIDSVLFEFNFAKDELQKNSF"
     gene            10660..>11129
                     /locus_tag="PK218_08095"
     CDS             10660..>11129
                     /locus_tag="PK218_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW98507.1"
                     /translation="MAKINNHPCYSEGAHQHYARIHVAVAPACNIQCNYCNRKYDCSN
                     ESRPGVTSSKLQPEEAVKKILFVGGEIQQLSVVGIAGPGDALANPEKTFKTFKMLYEK
                     APDLKMCLSTNGLMLPDYIDQIVKYNVDHVTVTINSVDETGEVGSRIYPWIHWN"
BASE COUNT         4033 a         1585 c         1907 g         3604 t
ORIGIN      
        1 ctaattaatc gtatttttgc ctcgtttttc acaactacaa caacacaaca actaaactaa
       61 tgaatcaaac aaaatacatt tttgttactg gcggtgtgac ttcttctctt ggaaaaggaa
      121 tcattgctgc gtctttggca aaactacttc aagccagagg ttatcgaacg accattcaaa
      181 aatttgatcc ttacattaat gtcgatccag gaacattaaa tccttatgaa catggcgaat
      241 gttttgtaac agatgatgga gcagaaactg atttggattt aggtcattat gaacgatttt
      301 tgaatgtacc aacatcgcaa gccaataatg ttactacagg aagagtgtat ctttcagtaa
      361 ttgaaaaaga acgtcgtgga gagtttttag gcaaaacagt tcaagttgtt cctcatatca
      421 ctaatgaaat taaagacaga atgcaattgc ttggaaaatc gggcaatttt gatattgtaa
      481 ttacagaaat tggtggaaca gttggtgata ttgaatcact accttatatt gagtcagtgc
      541 gtcaattagt atgggaattg ggcgaaaaca atggaattgt aattcattta acgctagtgc
      601 catttcttgc cgctgctggc gaattgaaaa caaaaccaac acaacattcc gtaaaaacgt
      661 tgatggaaag tggaatcaaa gctgatattt tagtgtgcag aacggaatat gaattgtctg
      721 aagaattacg tcaaaaattg gcgttatttt gcaacgtaaa acgtgaagca gttattcaat
      781 ctatcgatgc atctacaatt tatgaagtgc ctaatttaat gcttgaagaa ggtttggatg
      841 tagtagcttt aaaaaaatta agtttatccg aacaaaaaat acccgattta accaattgga
      901 atacatttct tgaccgatta aaaaatccaa aatacactgt taatgttggg ttggttggaa
      961 aatatgttga attacaagac tcctataaat cgattttgga agcttttatt catgcaggtg
     1021 ctgccaatga aactaaagta aatgtaattt ctattcattc tgaatacttg gatgccaatt
     1081 cggcagacaa gcaacttaaa aatctagacg gcatacttgt tgctccaggt tttggcgaac
     1141 gcggaataga aggtaaaatt gaaaccgttc gttatgcaag agaaaataat attccatttt
     1201 ttggtatttg tttaggaatg caaatggcag taattgaatt tgctagaaat gtagttggat
     1261 tgaaagaggc gaattctacc gaaatgaatg aaaacacacc aaatccggta atttcgatta
     1321 tggaagaaca aaaaaacatt acggataaag gtggaacaat gcgtttgggt tcttggaaat
     1381 gtgatttaga caacaactct ttggcacatt cgatatataa taagttacaa attgaagaac
     1441 gtcatcgtca tcgttatgaa ttcaataata aatatagaag tcaattagag gaaaatggat
     1501 tgaaagcatc tggtgtaaat ccggatactg gtttggtaga aattatcgaa ttagaaaacc
     1561 atccgttttt cattggtgta cagtatcatc cagaatataa aagcaccgtt gccaatccac
     1621 atccaatttt tgttagcttt gtggcggcaa tgaaaaaaca taaaaacaat taaaagttac
     1681 tgaacgcgtt cagcattatt atggaacaaa aaaataaatt tgatttaaat tcattaatag
     1741 gttttgcctt gatttttgtg attatcattt ggataatgac caatagtaaa aattcggaag
     1801 agcaaaaaca actagacaaa gtaaaacaag aacaagttga taaggaaaag aaaaccgttc
     1861 ccgcacaaac aattgctgaa acactaaaag actctacagt tacagatact gccaaagttg
     1921 aaaaattaaa atctgtttta ggaagttttg cctattctgc atcgttgcct tctgcaaaag
     1981 atagtattac taccatagaa aacgaattat tagttttaaa agtagctaat aaaggtggct
     2041 atatcagcga agttacgttg aaagactata agcgcttcga taaaaactct aaagaattag
     2101 tttcgttaat aaagaataac aattcaaatt ttaatttaga attaaaaaca aatgacaata
     2161 gaatattaaa ttcaaaagat ttatattttc aaccaacatt gactaaaaat ggcgaaagtc
     2221 agattctttc aatgaagtta aaagctggtc caaatgaatt tttagaatat aaatatgtat
     2281 tgaacaacaa aaactacatg ttggattttg aaattcaaac acaaggatta gctaaagttt
     2341 taaatacatc taaaccttct aatttagaat gggatttaaa agcctatcga aatgaaaaaa
     2401 gtatttctta tgaaaacaga tatgcagagt tgtattttga acataaaaat ggaaaagttg
     2461 attacgctgg tcttggctca caagaagata aagatttaga aaatgcaaca ttcattgctt
     2521 ttaaacaaca ttttttctct tcaattttat tgacaaaaac gccaattgaa ggagcaagca
     2581 ttcattcaac aaatttggtt catgatgaag ataaagacac catctttaca aaacaattca
     2641 aaactatttt accattagcc tataaaaatg gtgagttaga ttataaaatg aattggtatt
     2701 ttggaccaac agactataaa attttaaatt cttatgaccg aaatatagag cacattgttc
     2761 aattaggctg gggaattttt ggttggatta acaagtttat tttcgttcct ctttttggtt
     2821 ttttaagtaa cttcatgtcg cttggcatca caattattgt atttacaatt ttgattaaac
     2881 ttgccatgtc gcccattaca tttaaatcat ttttgtcaca agcaaaaatg aaagtattgc
     2941 gtcctgaaat tacagaactt ggtgaaaaat ttaaaaaaga tccaatgaaa aaacaacagg
     3001 aaaccatgaa attatacaac aaagctggag taaatccgat ggcaggttgt attccggcat
     3061 tgattcaaat tccttttgtt tatgcgtcat ttcagttttt cccatctgca atacaattgc
     3121 gccaacaaag tttcctttgg gcagatgatt tgtcttcatt tgatcaaatt ttcaaattgc
     3181 cttttagaat tccaatgtat ggcgaccatg tgagtttgtt cccaattttg gcagcaattg
     3241 caattttctt ttatatgaaa atgacttcgg gagatcaagc tatggcacaa ccacaacaag
     3301 aaggaatgcc cgatatgagc aaaatcatga aagtgatgat ttatatttca ccattaatga
     3361 tgttgatttt cttcaatagt tatggttctg gtttgagttt gtataacttt atgtccaatt
     3421 taattactat tggaatcatg ctggtcatca aaaagtattt tattgatagc gataaaattc
     3481 atgctcaaat tcaagaaaac aaaacaaaac ctaaaaaaga aagtaagttc caaaagaaaa
     3541 tgcgtgaaat gatggaacaa gctgaagcac aaaagcaatt gaaaaataaa aagtaaagtt
     3601 tttttaatgt ttattaaaag ttctgttttt tgaagtttat ctttaacaaa cagaactttt
     3661 ttgctttcac caaaaatgaa agtggtaatt agtaggttat agaaatatga ggaaaaccta
     3721 tgctaacagt tttattagct ctaggtttct taaaacaacg gatatttttg catttgtaaa
     3781 agaaaattca atttcaaatc ataatgaagt acttcttaat caaaatagaa ttacaaaacc
     3841 gtccattatt ttaggtgatt ttgatattgt tattctttct gttaaaccac aagatttcaa
     3901 gtcggtagca aaacaaatta aatcatttta aaaaatcagt tatatttcct gtcatggtag
     3961 gtgtaactat taagacgatt tctacagtag tatattgttc aaaagtactg ccttccatgc
     4021 caaatactcc aacgcaaata ggacaatgac tgtttttata gcatcaacta ctgttgaccg
     4081 aaagaactat ttattattca aaacctaatc aatactactg gaaaatcggt ctttgtagaa
     4141 gatgaaaatt taattaatgc tgctactgcc agttctggtt ctggtccagc ttatgttttt
     4201 tattttatac aagtatgata aatgcagctg ttgaacttgg atttaatgaa tcagaagcag
     4261 agttgtggta atcaaacttt tttgtggttt attattaatt aaaaacagta ttacactttc
     4321 aaatacggaa cggatataag ggtagcttca aaaggaggaa caaccaaaag tgcactccat
     4381 gtttttgaaa aacaaaatta tttaaggaat tttagtagca agcaaactta aggaagaatt
     4441 aggttttata aaaaaaagct ccaaaaaatt tggagcttta ggtattcata gcaataattt
     4501 tattagttca aatttggcaa taatacttta tcaattgcat gaacaatacc gttagaacac
     4561 tgtacatctg ttaaaacaat atttttattt acacttgatc cagaatcatc aattgaagtt
     4621 cccgatatac taaatgattg tccttgtaaa gtagtgatgg ctccagtagg aattgcattt
     4681 gataaaacat ttgcatttcc aactacatga tataataaaa ccgcggtttt gttcgcatct
     4741 gataaacctg tgtataaatt taaagtagcg gtatcaaatg ccgaatttat tggtgcaaaa
     4801 actgtaaatg gtgaaccagt agttccatct aaagttggta ctaacccttg gtcaactaat
     4861 aaaccagtaa caatacttaa attaggattt gcaattgcat ggtttttaat tgttggtaat
     4921 cctatcactc tgtcaacaac gtgaataact ccatttgatg caagaatatt agcactagtt
     4981 aaaattacat ttgcacctcc attactaaca cctccattaa tttttacccc agcagttaaa
     5041 tcaatataca tacttaaggt atttgttgac gatgcagttt cttttgctaa agttttatag
     5101 taaccagtca ttagttcaga actcgtaaat gattctgata aaacatgatt tttcaagatt
     5161 tcagtcaaaa ttggaactgg aacatcatta atggttgcat aaggcgttgt agctaaaaaa
     5221 gttgcaaatg cagcatttgt aggtgcaaaa acagtaaaat ctccactact gttaaatgta
     5281 tttactaaat tagttctttg tagtgcttcc actaaagtgc ttaagtctcc atttgctgaa
     5341 gcaatttcag caatagtttt tggttgtgaa ttgttgtcat cattactaca tgacggtaga
     5401 gtaattgcga ttgcaaaaac cattaaagat ttaaatagat ttttcataga tattgatttt
     5461 aaattaatgt tttgtttaat tttttttgta aaagtataaa caaatgttta ataaaaaaag
     5521 caaaatgtta aacaaaataa atttttataa ataaattaac agtattgttt agcataaatg
     5581 attttgaaaa tcagtatgta accatttttt gctacaattt tttttttgtt gttgtataaa
     5641 agttaaacaa tttataataa aaaaaaaata tctttaaaaa tatacttttt tcatactatt
     5701 tacgttatta ttcataatac tcttaaatca cttataatga aaaaaatatt attgctgtat
     5761 atactaagta cctcaattct ttattcccaa gatataaaca agttagataa taacggaaaa
     5821 aaaaatggtt tgtggaaagg tgttcacgaa gaatcgcaac gaccaagata tgaaggaaca
     5881 tttaatcacg gcaaagaagt aggtgttttt aaattttttg atgatactaa ggctggaact
     5941 gttattgcta ctagagaatt taacccaaaa gatgattctt gctatactat tttttacaat
     6001 caaaaaaata ataaagttag cgaaggaaaa gtgatcaaca agcaatttga aggtgaatgg
     6061 aaatattatc atgaagattt acccattatt atgactttag aattttatgt aaaaggaaaa
     6121 ttaaatggtg ttcgaaaagt atttttcaaa agtggagaat tagccgaaga aaccaattac
     6181 attgatggag ttaaaaacgg aacttcaaag atttatctcg aaaatggtgt ggtaatagaa
     6241 gatactaatt ataaaaatgg agaatatgat ggaaaagcca tttttagaac ttcggacaat
     6301 caaatttcag ctgaaggtgt atttaaaaat ggaaaaaaag ttggtatttg gaaaattctt
     6361 gaaaaaggaa ctttgaaaaa tgttaacatg aaccttcaag gaaagaaatt ccaaaaaaga
     6421 gttaaaccat tagaagaaca ataaatatta aagtaatttt gctgtaaatt tttttacaat
     6481 aaaatgaaac gagttgtagt tggactttca ggaggtgttg attctagtgt tgctgcttat
     6541 ttgttaaagg aacaaggata tgaagtcata gggcttttta tgaaaaactg gcacgatgat
     6601 tcggtcacta tttccaatga atgtccttgg ctagaagata gtaacgatgc gcttttggtt
     6661 gccgaaaaac tcggaattcc atttcaaacg gttgatttaa gcgaacaata caaagaaaaa
     6721 atcgttgact atatgttcaa cgaatatgaa aaaggacgca ctccaaatcc tgatgtattg
     6781 tgtaataggg aaataaaatt tgatgttttt atgaaaatcg cgctttctct tggtgccgat
     6841 tttgttgcga ctggtcatta ttgtagaaaa ggtgaaatta aagtggatgg aaaacctgtc
     6901 tttcaattat tagctggaaa agatgagaat aaagatcaat cttatttcct ttgtcaatta
     6961 tctcaaaatc aattgactaa agcattgttt ccaataggag aattgacaaa acctcaagtt
     7021 agagaaattg ccggcaaatt agacttaatt acagccgaaa aaagagattc tcaaggatta
     7081 tgttttattg gaaaagttag attgcccgaa ttcttgcaac aacaattaca acccaaagaa
     7141 ggatttatct atgaaattga tgctcaaagt gaaatctatt caaaagcaat tccagagttt
     7201 aaatccttag aagaacaatt gatttttgaa tcaacttcga ttacctattt acccgaaaac
     7261 ggaaaagtag ttggaaaaca tcaaggtgca cattatttta ccataggaca acgcaaaggg
     7321 ttgaatgttg gcggaacaaa agaagctctg tttattattg caactgatgt cgattctaat
     7381 gtaatctata cgggtcaagg tcacaatcat cctggtttgt ttaaaaaaac attaaaagtg
     7441 caaccttcag aagttcattg gattcgagaa gatttaaaat taaaaaatgg agaatcttta
     7501 aatgtgaaag ccagaattcg ctaccggcaa gaattacaaa atgctacact atatcaattt
     7561 gaatctggat tatatgtttc ttttgaaaat ccacaatctg ctattactga aggccaattt
     7621 gtagcttgga atattgatga cgaattagtt ggttcaggag taatttcgta atttagtgct
     7681 ttcaatttag aaagctatga aaaaaattct agtatcattt ttattggtat cattcttaaa
     7741 tgcattttct caagaagatg cgtgggttta ttttaatgac aaaccaaatg cacaaacttt
     7801 tttggacaat cctttgtcaa tgttatctca aagagcttta gacagaaggg agaatcaagg
     7861 aataacgttg acagttcaag atactcccat ttacgaacct tatgttgatc aaatttcaaa
     7921 tgcagatgga ataactgtaa tggcaaaatc caagtggtta aattgtgttc acgttcgagg
     7981 agaacttaca aatattcaat ctttgacaaa tttttctttt gtaaatcaca ttcgttttgc
     8041 tgataattct ttgaataata gattttcaaa tccaaatcca attcaggtta taaacaaaca
     8101 attggatgtt caatccaatt ttaattacgg aaattcaacg aatcaaattc aaatgttgaa
     8161 tgctcaaata ttgcatcaac aagattatac tgggcaagga aaaataattg cagtattgga
     8221 ttcaggtttt ataaatgtta actctactgc accgtttcaa cgattgtttg ataataattt
     8281 gattcttggt ggttacaatt ttgtttcgca caattcagat gtttattcgc ttcacaatca
     8341 tggaacgagt acactttcat gtatgggagc atttgtagat ggtcaacttg ttggtacagc
     8401 tccagatgca agttattatc tttttgtaac cgaagatgtt gctgccgaaa atccagtaga
     8461 agaaagttac tgggtacaag cagccgaaga gtcagataga ctaggagcag atgttatttc
     8521 aacgtcactt ggatattttg aatatgataa tccaaattat agttatcaat atagtgattt
     8581 aacgggtgac catgctttta tttcgcaagg agccaatata gctttttcca aaggaatgat
     8641 agttgtagca agtgcaggaa attctggcgc aacaaacaat ccaaatattg gagtgccagc
     8701 agaagccact aatgtacttg cagttggtgc tgttcaagcc aatgaaacct atgcattatt
     8761 tagttcaatt ggaccaagtt tcgacggaag gataaagccg gatgttatgg cacaaggaca
     8821 acaatctact gttgcaaata ctgcaggaca aataacttcg gccaacggta catcattttc
     8881 atgtcctata atggcaggtg ccatcacaag tttttggagt gctattcctt gggcaacgaa
     8941 ccagcaagta cttgattttg ttaaacaatc tgcagatcga tttttgtctc ctgacgatca
     9001 atttgggtat ggaatacctg attttcaaat cgcgcttgct atggcacaat tgtcaacaaa
     9061 tgaaagtttg attaaaaagt ttacacttta tccaaatcca acaaatgata aaatttcaat
     9121 aaattttcca aatactattg atgagataaa ttttatttta tataataatt tgggtcaaaa
     9181 aataattgaa aaaaacatta aaaatgaatt tcctcaaatt tcacttgaaa ataattcgtc
     9241 aggaatatat ttttatacta ttcaatctaa aagtgatttc caatcgggaa aaataattaa
     9301 aaattaaagg tttttaaatg aataaaatta cacaactttt caatataaaa tatccaatta
     9361 ttcaaggcgg aatgatatgg aacagtggct ataaattagc aagtgccgtt agtaatgctg
     9421 gcggtttggg attaatagga gcaggctcaa tgtatccaga ggttttaaga gaacatattc
     9481 aaaaatgcaa aaaagcaaca aacaaacctt ttggggtgaa cgttcctatg ttgtatccta
     9541 atattgaaga aattattcaa attattatag aagaaggtgt taaaattgta ttcacttctg
     9601 ctggaaatcc aaaaacatgg actagtcact tgaaagaaaa cggcattaca gttgttcatg
     9661 ttgtgagtag ttccaaattt gctttaaaag cccaagaagc tggagtagat gccgtagttg
     9721 cagaaggatt tgaggccggt ggacataacg gaagagaaga aacgacaaca ttaacattaa
     9781 ttccaatggt taaagaaaaa attcaaattc cattgattgc tgctggtggt attgcaactg
     9841 gacgcggaat gttggcaact atgatacttg gtgctgatgg ggtacagatg ggtagtagat
     9901 ttgctgcttc aattgaaagt tcagcacatg aaaattttaa aaaaacaata actgaaactg
     9961 gtgaaggtga tactcaatta actttaaaag aattagcacc agttcgattg attaagaata
    10021 aattctataa cgatgtacaa gaattatatt ccacttgtcc ttcaaaagat gcgttagaac
    10081 agctgcttgg aaaacgtagg gcaaaacgcg gaatgtttga aggagattta ttagaaggtg
    10141 agttagagat tggacaaatt gcaggtatta ttcacgacat ttcaactgta gagcaaatta
    10201 ttgattctgt tctttttgaa tttaattttg caaaagacga gctacaaaaa aacagctttt
    10261 aattgcatta aatttcattc ttacaaataa ttttccgtca taagttatta acacattttt
    10321 ttaaatattt aatctaaaat agtattgata actttatttt aaatatttct tgtttattaa
    10381 acacttgttt taaaggtgtt tatgtgtttt tttaaatttt tatttctcat gatttatgtt
    10441 tattagtttg tttataacat tttaataact ctttttttat ctcttcttga actttcattt
    10501 ataatccttt ttttatatga agtttattat gcatttttca ttcctaggtt ttctaaggaa
    10561 cacattgtgc attacaattt ggaaataatt aataaggagt tatctatgag ttgttcgtgt
    10621 acttcaagta gttctcaaga atcaatccaa gaagaggtaa tggcaaagat caataatcat
    10681 ccttgttata gtgaaggtgc tcaccaacac tatgcaagaa ttcatgttgc cgttgcacca
    10741 gcttgtaata ttcaatgtaa ctactgtaat agaaaatatg attgttcaaa tgaaagcaga
    10801 ccaggagtaa catcttctaa attacaacct gaggaagcag ttaagaagat tttatttgta
    10861 ggtggagaaa ttcaacaatt gtcagttgta ggaattgctg gacctggtga cgcgttagca
    10921 aatcctgaaa aaacttttaa aacttttaaa atgctatatg aaaaagcacc ggatttaaaa
    10981 atgtgtttat ctacaaatgg attaatgctt ccagattata ttgaccaaat tgtaaaatac
    11041 aatgttgacc acgtaactgt tacaatcaat tcagttgatg aaacaggaga agttggttca
    11101 agaatttatc cttggattca ctggaatca
//
LOCUS       DAOOBH010000074        11098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_7481_length_11098_cov_5.112922, whole genome shotgun sequence.
ACCESSION   DAOOBH010000074 DAOOBH010000000
VERSION     DAOOBH010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7481_length_11098_cov_5.112922"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3086)
                     /gene="cas9"
                     /locus_tag="PK218_08100"
     CDS             complement(<1..3086)
                     /gene="cas9"
                     /locus_tag="PK218_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HPW98508.1"
                     /translation="MARILGLDLGTNSIGWALIDDKQNKILGVGSRIFPMGVENLGDG
                     DGEISKNASRTGARGVRRQFFRRRLRKKILLKALSEYGMCPLIQSDFEDWKKTKAFPS
                     EKLVAWFALNPYELRHKGLHEKLSLEEIGRLFYHLIQRRGFLSNSRKGGSDDGAIFKG
                     NPKEGKIGIDATLDSLEGKTLGSYLYSIYPKENEPFQNGLERIRNRYTTRKMYVDEFE
                     VLWHAQSKYHASLTAELKALFGGRKLDGYKEDGILFHQRPLRSQKHLVGNCSFEPSKT
                     KCPISAIPVELFRIYQWVNTVEYNGKKLAKTEKDKMIEVLLTSEKDEFKKLRKAIGKE
                     SAEFKFNYKDDDKIVGTHTISNLSNKKYFGKRWFDFTDKEQEDIWHVLYFFDSKSNLK
                     EYALKNWNFTEEQAEAIAKFNVKDGYASLSRKAIGNILPFLQQGFVYDIAVALGGIKN
                     ALGTEWETLSEDKKSFIIDNVPDIVRAKISGGYIDTIKDLLRKEFGFTDATLKKLYHH
                     STNIDATTLLDRLPVGKEADKELQSLKNPIVVTALFELRKLVTALIDEYGSIDEIKVE
                     MARDLKLSKMQRNKIRREQKRLEKENDRVKDRLKEEGQRITHDNILLYKLWEECKKTC
                     PYTGQPIPLHKLFTGEIQIEHIHPWSRSLNDSFSNKTLCYADENRRKGDKTPFEFYGD
                     DEGNWSAIKERALKLFSDTKEYPNSYQKFKRFVQIKFDEDFSTRQLNDTRYISKEAKE
                     YLSQICPKVIVSPGQATSNLRQKWGMNNILNDENEKTREDHRHHAVDALVMACTKVSY
                     VQELSKWNRYNRNSELKEFPLPWEGFRREAELAIEKILVSHKRTSKDITVRTHTIEKN
                     GVKYKNVGVAARGQLHKEFVYGKRKAPFSDEAFHIRKPIESLLTEKHIEKVVDTTIRN
                     LIIKKVNEMGGFDKDKIPANTFFIVDDKGVKQPQIFLPNKNGQPVPILKVRIQETINK
                     AKQLKNNSNQFVNPRNNDHVLIYETIDGKLEKDVVSFWEVVERKKQGLDKIKLPIDG"
     gene            3495..5114
                     /locus_tag="PK218_08105"
     CDS             3495..5114
                     /locus_tag="PK218_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW98509.1"
                     /translation="MLTVSNLSVQFGKRILFDEVNTTFTQGNCYGVIGANGAGKSTFL
                     KILAGDIDPTSGRVILEPGKRMSVLNQNHNMFDENTVLETVMMGNKVLFAVKSEMDAL
                     YADYDDKNADRIGELQVQFEEMNGWNAESDAGAMLSNLGIAADMHYTLMGELEGKMKV
                     RVLLAQALFGNPDVLVMDEPTNDLDFETITWLENFLANYENTVIVVSHDRHFLDSVCT
                     HISDIDFSKINHYSGNYTFWYESSQLAARQKAQQNKKAEEKKAELEEFIRRFSANVAK
                     SKQATSRKKMIEKLNLDEIKPSSRRYPAIIFDTEREAGDQILNVQGLSASVDGEVLFK
                     NVDLNMAKGDKIVVFSKDSRATTAFYEILNNNLKADAGTFEWGITTTQSYLPNDNSEF
                     FTDGSLNLVDWLRQWVKTEEERDEVYVRGFLGKMIFSGEEALKKCTVLSGGEKVRCML
                     SRMMMIRANVLMLDEPTNHLDLESITAFNNSLKNFKGSVLFTTHDHEFAQTVANRVVE
                     LTPTGVIDRYMTFDEYLDDEKVKELRKKMYS"
     gene            complement(5225..5887)
                     /locus_tag="PK218_08110"
     CDS             complement(5225..5887)
                     /locus_tag="PK218_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98510.1"
                     /translation="MKTLKLSILAIFVSASFLSCDNSKKQEAEKNVAQFSNYVDSVSV
                     VSTEKLKENWENVENAYTEKKLQAQSSIESLGENPELNEKLNKVNSKYDEFKAKFQID
                     AEKLEASNRKMNFRKTFFDGKEIGDDMNFDWVNKDNIVSVYDLFVTTASKNKDSYSRE
                     DWDEIKLMYEALDTRKNTVEKEGLTSSDNLKIAKLKLKFAPMYTLNRMGAKSEENAEA
                     KK"
     gene            complement(5966..6313)
                     /locus_tag="PK218_08115"
     CDS             complement(5966..6313)
                     /locus_tag="PK218_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98511.1"
                     /translation="MKKINQTTLLDQKILELTKKRDFELMDLKNQYNIVIESAKPINI
                     IKNSFSSLQDTSLKKETLLELATSFLGGYLSKKILVGKSNNTIKRVIGNVLQYSISTL
                     INKFNNFKHQKNN"
     gene            complement(6319..6696)
                     /locus_tag="PK218_08120"
     CDS             complement(6319..6696)
                     /locus_tag="PK218_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98512.1"
                     /translation="MENIATNIEKLYDKATLYAKTSYELVRLKAIDKISEIISSLAVV
                     ISIVFIVAMFTLFLNVGIALWIGQTLNNISLGFFIVSGFYILLAIIVFIYRKKLIKEP
                     IDNLIVGKLLEEKIRERNDNNLK"
     gene            complement(6710..6979)
                     /locus_tag="PK218_08125"
     CDS             complement(6710..6979)
                     /locus_tag="PK218_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPW98513.1"
                     /translation="MKASNIVLGIVGGLAAGAVLGILFAPDKGSNTRKKIAKKSSDLK
                     DNVKDSFNDFISNVEDKYKDLTSKASETVDDLSDNIEKMNKELKR"
     gene            complement(7020..7175)
                     /locus_tag="PK218_08130"
     CDS             complement(7020..7175)
                     /locus_tag="PK218_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HPW98514.1"
                     /translation="MSNLLYTVAVILVILWALGFFVYSFGNITHILLVIAIIAILLRL
                     IKGNKPV"
     gene            complement(7204..7662)
                     /locus_tag="PK218_08135"
     CDS             complement(7204..7662)
                     /locus_tag="PK218_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98515.1"
                     /translation="MKKLYTSMVLVLLTVSSYSQKTTYTSNNTIQENNQIVALEDNQI
                     VSMEVSENSAKEDEFRYYYFPNMFAYYDLETNKYIYKENNEWKTSSELPEYYGGYSLF
                     KNTRIPIRNYIGDNPQEKIKEHKKEFPYIKKSRMLKSLNFNGNTAISSIN"
     gene            complement(7940..8896)
                     /locus_tag="PK218_08140"
     CDS             complement(7940..8896)
                     /locus_tag="PK218_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPW98516.1"
                     /translation="MSAIKTYFKSFIKVLKNTFNGFLEYKVLKISASLAYITVFSLAP
                     LVLVVLFIFDFFWGKEMIENLIFDNAKDFVGSATAGQLKEMIQNLTLTNNSTFSAVIG
                     VVSLVFGATSLFAEIQDSINTIWGVRPKKQSGIWLFIKSRLLSFGVIGSLGFILLVAL
                     GFSTLLDSLNAYLSERYSESVLYVIYGINILFTIFITSLLFASIFVILPDAKIKWRQV
                     RLASIATTVLFLIGKFLISFYITSSNVNTIYGAAGSIVVLMLWVYYSSVILYLGATFA
                     KCYAIEFSTAIKPSEFAEIVKKVEVSSEAQTLQQAENNTKTD"
     gene            complement(8900..>11098)
                     /locus_tag="PK218_08145"
     CDS             complement(8900..>11098)
                     /locus_tag="PK218_08145"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPW98517.1"
                     /translation="IRIINTFKSRIFLNIAFVISLASIFFTASITYKHIVELNKSTDL
                     VMKSYEVKLNIENLMSELKDAETGFRGYLLYKDKAFLNPFNNSRENVDKAFIRIKRLT
                     KENKTQQKNLRELYRLIAIRYSYFDEYREIKSDYKYQKYFIANSKKAMDNVRKKTDEM
                     SKLEDYLLEKREKSFKDTSFYTPFYALLIIIITLFLIVVAYIRINKDLISLRSSNDRL
                     MVINESQKLAEIIGKFGSWEVNLERNSYQFSDNLYRLLGYEPQEFEPTLEEYLKTIHP
                     DDLDFVKTKSANILELKELPGYTYRIIKKDGEIRFFHATGILIQNNFGENILVGTTQD
                     VTDHQIYTEKIKKNNEELIEYNKRLKIYDESSKQAEILGNYGSWILNFKTLEFIYSDN
                     KFRLLGCEPQSFNPSIENLLEFVHPDDKHIIVNGNQSIIDKGELPTINYRIIRKDGEL
                     RNFRTTAKIYTDSLGTKNMIGTTQDITEEILKTNDLKYKNLELERSNKELIEFNYAAS
                     HDLQEPLRKIQTFISRINDNEIENLSEKGKEYMDRIVNAASRMRILIDDLLQYSRTNK
                     SDALFETINLNETLSNVTQDLSETILENETTIHLPKLPTIKGIPFQIQQLFINLISNS
                     IKYRKPEVNPIISIAYEKVKAKTENIADDSNSLYHKFIVNDNGIGFEPEHAEKIFLLF
                     NRLHGKTDYPGTGVGLAICKKIISNHNGYIQAIGKPNEGATIIIYIPVDL"
BASE COUNT         3461 a         1819 c         1612 g         4206 t
ORIGIN      
        1 ccatcgatag ggagtttaat tttatctaaa ccttgctttt tcctttcaac aacctcccaa
       61 aaactcacaa catctttttc tagttttcca tctatggtct cataaattaa aacatggtca
      121 ttatttcttg ggtttacaaa ctgatttgaa ttattcttta attgtttggc tttatttatt
      181 gtttcttgaa ttcttacttt caaaattgga actggttgtc cattcttatt aggtaagaaa
      241 atttgtggtt gcttaactcc tttatcgtct actataaaaa aggtgttggc tggaatttta
      301 tctttatcaa aaccacccat ttcattaact tttttaataa ttaaatttct aattgtagta
      361 tcaaccactt tttcaatatg tttttctgtc aacaaacttt caataggttt tctaatatgg
      421 aaagcctcat cactaaaagg agcttttctt ttaccgtaaa caaattcttt atgcaattgt
      481 cctcttgcag caactccaac atttttatat ttcactccat ttttttcaat tgtatgagtt
      541 ctaacagtta tatctttact tgttctttta tgggatacca atattttttc aattgccaat
      601 tcggcttctc gtcgaaatcc ttcccatggt agcgggaatt cttttaattc cgaatttcta
      661 ttataacggt tccattttga taactcttga acataggaaa ctttagtaca cgccatgacc
      721 aacgcatcaa ccgcatgatg tctatgatct tcacgtgttt tttcgttttc atcattcaat
      781 atattgttca ttccccattt ttgacgcaag tttgaagtgg cttgtcctgg cgatacaatt
      841 acttttgggc atatttgcga taaatactcc ttagcttctt tactgatata gcgtgtgtcg
      901 tttaattgtc gagtgctaaa atcttcatca aacttaattt gaacaaaacg cttaaacttt
      961 tgataggagt taggatattc cttggtgtct gaaaacaatt tcaaagcacg ctcttttata
     1021 gcgctccagt ttccttcatc gtcaccataa aattcaaatg gtgttttatc gccttttcgt
     1081 ctgttttcat cagcataaca caaagtcttg ttgctaaaac tatcgtttaa cgaacgactc
     1141 caaggatgaa tgtgttctat ctgtatttcg cccgtaaaga gtttatgtaa tggtattggt
     1201 tgcccagtat aaggacacgt ttttttacat tcttcccata gtttatacaa taatatattg
     1261 tcatgcgtta tgcgttgtcc ttcttctttc aatcggtctt ttactcggtc attttctttt
     1321 tccaatcgtt tttgctctcg tcttattttg ttacgctgca ttttcgaaag ttttaaatca
     1381 cgagccattt ctacttttat ttcatcaata cttccgtatt catctatcaa agcagtaacc
     1441 agttttcgca attcaaatag tgccgtaact acaatagggt ttttaagcga ttgcaattct
     1501 ttatcggctt ctttgcccac aggcaatcgg tctaacaaag tggtagcatc aatatttgtg
     1561 gagtgatggt atagtttttt taatgttgca tctgtaaatc caaattcttt tcgcaacaag
     1621 tccttaatag tatcaatata tcctcccgat atttttgccc tcacaatatc aggaacatta
     1681 tcaattataa aacttttttt atcttccgat aaagtttccc actctgttcc aagggcattc
     1741 tttataccac caagtgcaac agcaatatca taaacaaaac cttgctgtaa aaaaggtagt
     1801 atattgccaa tggcttttcg actcaaactc gcataaccat cttttacatt aaatttagca
     1861 atagcttcag cttgttcttc agtaaaattc cagtttttta gtgcatattc ttttaagttc
     1921 gatttactat caaaaaaata caatacatgc caaatatctt cttgttcttt atctgtaaaa
     1981 tcaaaccatc gttttccgaa atattttttg ttggatagat ttgaaatagt gtgtgttcca
     2041 actattttat catcgtcttt gtaattgaac ttaaattcag cactttcttt tccaatggct
     2101 ttgcgtaact ttttaaattc atccttttca gaagttagta gcacttcaat cattttatcc
     2161 ttttctgtct tcgccaactt tttaccatta tattcaacag tatttaccca ttgataaatt
     2221 cgaaatagct caacaggaat agcactaata gggcattttg ttttgcttgg ttcaaaacta
     2281 cagttaccaa ccaaatgttt ctgcgaacgc aacggtcgct gatgaaataa tattccgtct
     2341 tccttgtaac catccaattt tctaccacca aacaatgctt tcaattcagc agtcaaacta
     2401 gcatgatatt ttgattgcgc atgccacaaa acctcaaact catctacata catttttcga
     2461 gtagtatagc ggtttctaat acgttccaat ccattttgaa aaggttcgtt ttcttttggg
     2521 taaatagagt ataagtaaga acctagtgtt ttcccttcaa ggctatctaa agttgcatca
     2581 atcccaattt ttccttcttt gggatttcct ttaaagatag caccgtcatc gctgccacct
     2641 tttcggctat tgctcaaaaa tcctctgcgt tgtatcaaat gataaaacaa tcttcctatt
     2701 tcttccaaac taagtttttc atgcaaacct ttatgtcgta attcataagg atttaaagca
     2761 aaccatgcta ctaacttctc actcggaaat gcttttgttt tcttccaatc ttcaaaatca
     2821 gattgtatca atggacacat tccatattca gataaagcct ttaataatat ttttttgcgc
     2881 aatcttcgtc taaaaaattg tcgtctaaca cctcgagcac ctgttctact tgcattttta
     2941 gatatttctc catcaccatc acccaaattc tcaacaccca taggaaagat tctacttcca
     3001 actccaagaa ttttattttg cttatcatca attaatgccc aaccaatact atttgtacct
     3061 aaatctaatc ctaatattcg tgccataatt ttaaatgtat ttgtaagaat ataaaagtaa
     3121 ataaaaaaaa gtttacttgt ttacggtttt tccgtaaacg aactaaaaaa gttttttcat
     3181 atatttgctt tagattataa ttatcctaat tcttaatctt attacaataa ggctatatgc
     3241 cgtagatgaa aatctttagt cctgcttcgg tgggactttt tttatactat tgataaacaa
     3301 aggtataatt tatgtttcca tctatactcc acggttataa tactaaataa atagacacta
     3361 agtcgttatt tgtttcatag gcttccatga tggaagttta ggtgtctaaa aagtctcata
     3421 atctaacggg ttaagaagac taaaatatca taacaattca ttacttttgc acaaaataat
     3481 aaaacaagac tttcatgctt acggtttcaa atttatcagt tcaatttggt aaaagaatat
     3541 tatttgacga agtaaacact acgttcactc aaggtaactg ctacggagtt attggtgcca
     3601 atggtgctgg taaatctact tttctaaaaa tactcgctgg ggatatagat cctacctcgg
     3661 gtagagttat cctagagcca ggcaaacgta tgtcggtatt gaaccaaaac cacaacatgt
     3721 ttgacgaaaa cacggtgttg gaaacggtga tgatgggaaa caaagtactc tttgccgtta
     3781 agtcggagat ggatgctcta tatgccgact atgacgataa gaacgccgac agaataggtg
     3841 agttgcaagt acaatttgag gaaatgaacg gatggaacgc cgagtctgat gctggagcca
     3901 tgctttccaa ccttggtatt gctgccgata tgcactatac actaatgggc gaactggaag
     3961 gaaaaatgaa agtacgtgtc cttctagcac aggcactctt tggaaatccc gatgttctag
     4021 taatggatga gccaaccaat gacttagatt ttgaaaccat cacctggtta gagaatttct
     4081 tggcaaacta tgaaaacacc gtgattgtag tatctcacga tagacacttc ttagattcgg
     4141 tatgtaccca catttccgac atcgacttta gtaaaatcaa tcactactcc ggaaattata
     4201 cgttttggta tgaatcgagc caattagcgg caagacaaaa agctcaacaa aacaaaaaag
     4261 cagaagaaaa gaaagcagaa ttagaagagt ttattcgtcg tttttctgcc aacgtagcca
     4321 aatcgaaaca agctacttct cgtaaaaaaa tgattgagaa attaaattta gatgagatta
     4381 aaccatccag ccgtcgttat ccagctatca tttttgatac agagcgcgaa gcgggtgacc
     4441 agatattaaa cgttcaagga ctttcggcta gtgtagatgg cgaagtgttg tttaagaatg
     4501 tagatttaaa catggcaaaa ggcgataaga tagtagtttt ttctaaagat tcgagagcta
     4561 ctactgcctt ttatgaaata ctaaacaaca acctaaaagc agatgctggt acttttgaat
     4621 ggggaattac gacaacccaa tcctatttgc caaacgataa tagtgagttt tttaccgacg
     4681 gaagtttaaa tctagtagat tggttgcgcc aatgggtaaa aacagaagaa gaaagagatg
     4741 aagtatatgt tcgtggtttt ttgggtaaaa tgattttctc gggagaagaa gctctaaaaa
     4801 aatgtacggt actttctggt ggagaaaaag tacgctgcat gttgtcaaga atgatgatga
     4861 taagagccaa tgtattaatg ctagacgaac caaccaatca cttagactta gagtcgatta
     4921 cggcttttaa taattcgtta aagaacttca aaggttcggt attgtttact actcatgacc
     4981 acgagtttgc acaaacagtt gccaatagag tagtagaact aactccaact ggtgttattg
     5041 accgttatat gacttttgat gaatatcttg atgatgaaaa agtaaaagag ttacgcaaga
     5101 agatgtattc ataatatagc ataaaaaaac atttcaattg aaatagtttt tttgtttaaa
     5161 aatcaaaaag cagcatttta ttaaaaaatg ctgcttttct tcaacctaaa caaaaaccaa
     5221 tctgctattt ttttgcttct gcattttctt ccgattttgc acccattcta ttcagtgtgt
     5281 acattggagc aaactttaat tttaatttgg caatttttaa attgtccgaa gatgttaagc
     5341 cttctttttc aacagtgttt tttcgggtgt ctaaagcttc atacattaat ttaatttcat
     5401 cccagtcttc tctactataa ctgtctttgt tttttgaagc tgttgtaaca aataggtcat
     5461 aaacagatac tatattgtct ttgtttaccc aatcaaaatt catgtcatca ccaatttctt
     5521 taccatcaaa aaatgttttt ctaaaattca tttttcgatt tgatgcttcc aatttttctg
     5581 catctatttg aaactttgct ttaaattcat cgtattttga atttacttta tttagttttt
     5641 catttaattc ggggttttca ccaagcgatt ctattgaact ttgtgcttgt agtttttttt
     5701 cagtatatgc attttctaca ttttcccaat tttcttttaa tttttctgtt gaaacaacag
     5761 aaacagaatc tacataattt gaaaattgtg ctacattttt ttctgcttct tgtttcttgc
     5821 tattgtcaca ggataagaaa gaagcactga cgaagattgc tagtattgat aattttagtg
     5881 ttttcatttt tttttatttt aaattttttt gctaattgta ttaaatcata acaaatcaat
     5941 tttatttggt atcattggta caattttaat tgtttttctg atgtttgaag ttgttaaact
     6001 tgtttattaa agtcgatatg gaatattgta aaacatttcc aataactctt tttatagtat
     6061 tatttgattt tccgactaag attttttttg ataaatagcc accaagaaaa ctagttgcca
     6121 attccaataa agtttctttt tttagcgaag tatcttgtag gctagagaaa gaatttttta
     6181 taatgtttat cggctttgcg ctttcaatta ctatattgta ttgatttttt aaatccatca
     6241 attcaaaatc ccttttttta gtaagctcta gaattttttg atcgagtagt gtcgtttgat
     6301 taatcttttt catagcaatt attttagatt attatcatta cgttcacgaa ttttctcctc
     6361 taaaagtttg cctacaatca aattgtctat aggctctttg attaattttt ttctataaat
     6421 aaacactata atggctaata gaatataaaa tcctgaaaca ataaaaaatc ctaacgagat
     6481 attatttagt gtttgcccta tccaaagtgc aattccaaca tttaaaaata atgtaaacat
     6541 tgcaacaata aatacaattg aaatcacaac agccagcgaa gatattattt cggaaatttt
     6601 atcaatcgct ttgagtctta ccaactcata agatgtttta gcataaagcg ttgctttgtc
     6661 ataaagtttt tcaatatttg ttgcaatgtt ttccattata aatgatagat tatcgtttta
     6721 attctttatt cattttttca atattgtctg aaagatcatc aactgtttct gatgcttttg
     6781 aagttagatc tttgtatttg tcttctacgt tcgatataaa atcgttaaaa ctatctttta
     6841 cattgtcttt taaatcagaa ctttttttgg caattttttt tcttgtgttg cttcctttat
     6901 caggtgcaaa caaaattcct aaaactgctc cagcggctaa tccgcctacg attcctaaaa
     6961 ctatattact tgctttcata ttaaattatt ttaattaatt agtacttatt ttttgttttt
     7021 tatacaggtt tatttccttt gattagtctt aatagtattg cgattattgc tattactaaa
     7081 agaatgtgag tgatgtttcc gaagctatag acaaaaaaac ctaatgccca aaggattact
     7141 aaaattacag ctactgtgta caataaatta ctcatgattt ctatttttta atggttaatg
     7201 attttaattt atacttgata ttgcagtgtt tccgttaaaa tttaatgatt ttagcattct
     7261 tgattttttg atatagggaa actctttttt atgttctttt attttttctt gtggattatc
     7321 tccaatataa tttctaattg gaattcttgt gtttttaaat aatgaataac caccataata
     7381 ttcgggtaac tcggatgatg ttttccactc attattttct ttatagatat atttattagt
     7441 ttctaagtca taataagcaa acatattagg aaaataatag tatctaaact catcttcttt
     7501 tgcgctgttt tcagatactt ccatcgaaac aatttgattg tcttctaaag ctacaatttg
     7561 attattttct tgaattgtat tgttggaagt ataagttgtt ttttgcgaat atgatgatac
     7621 tgtcagcaag actaaaacca tcgatgtgta taattttttc atagcaaatt ttttattggg
     7681 ttactattct atttttgata ttacaaagtt ctttcaaatt tgatagtttc atgttatatg
     7741 attttatcag gttgttatag aatttcaatt tgattcattt taaactataa attttgcgct
     7801 aactatcaat gaacattaca aagttgcgaa gttttgccac ttattttgtt acactatttt
     7861 tgggttttgt tataaaattt cgtcaaaaaa aatccattat aaaaatggaa ttagatttga
     7921 tgaatttttg aggaatttct taatcagttt tggtgttgtt ttctgcttgc tgcagtgttt
     7981 gtgcttctga ggatacttcc acttttttta ctatttctgc aaattcagag ggctttattg
     8041 ctgttgaaaa ttctatggca tagcatttgg caaaggttgc tcctaaatat agaataacag
     8101 aagagtagta cacccaaagc attaacacca ctatagatcc ggctgctcca taaatagtat
     8161 ttacattgga acttgtaatg taaaatgaaa ttaaaaattt accaattaga aataagacgg
     8221 ttgttgcaat tgaggctaat ctaacttgtc gccattttat ttttgcatca ggtagaatta
     8281 caaatatgga agcaaataag agtgaagtaa taaaaatggt aaacagtata ttgataccat
     8341 aaataacata aaggactgat tccgaataac gctcagataa gtaagcgttt aacgaatcta
     8401 ataaagttga aaacccaagt gccactaata atataaaacc taaacttcca ataacaccaa
     8461 aagacagtaa tcttgattta ataaataacc atatccctga ttgttttttg ggacgaacac
     8521 cccaaatggt atttatagaa tcttgaattt cggcaaataa ggaagtagca ccaaaaacta
     8581 aggaaactac tccaattact gctgaaaaag tagaattatt ggtaagggtt aaattttgaa
     8641 tcatctcttt taattgacca gcagttgcac ttccaacaaa atcttttgca ttatcaaaaa
     8701 ttagattttc aatcatttct tttccccaaa aaaagtcaaa aataaataat acgactaaaa
     8761 ccaaaggagc caaagaaaat actgtaatat aggctaatga agcacttatt tttaaaactt
     8821 tatattctaa aaaaccatta aaagtgtttt ttaaaacttt aataaaggat ttaaaatagg
     8881 ttttgatagc actcattttt tataaatcaa caggaatata gataataata gttgcgcctt
     8941 catttggttt tccaatggct tgaatgtaac cattatgatt gctgatgatt tttttgcaaa
     9001 tagccaaacc aacaccagtt cctgggtaat cagtttttcc atgaagacga ttgaacagca
     9061 aaaatatttt ttctgcatgt tcaggttcaa aaccaatgcc gttatcatta actataaatt
     9121 tatgatatag gctgttagaa tcatctgcaa tgttttcggt ttttgctttt actttttcat
     9181 aagcaattga tattatcgga tttacttcgg gtttcctgta ttttattgaa ttactaatta
     9241 aattaataaa caattgttga atttgaaatg gaattccttt tatagttggt agtttaggta
     9301 agtgtatagt cgtttcattt tctaaaatag tttcagacaa atcttgagta acatttgata
     9361 aagtttcgtt caaatttatt gtttcaaata gagcatctga tttattggtt cttgaatatt
     9421 ggagtaaatc gtcaataagt attctcattc tactagcagc attcactatt ctatccatgt
     9481 attcttttcc tttttcgcta agattttcaa tttcgttgtc attaattcta gaaataaagg
     9541 tttgaatttt tcgtagtggt tcttgtagat catgacttgc ggcataatta aattctatta
     9601 attctttatt tgaacgttct aattccagat ttttatattt taaatcatta gtttttagta
     9661 tttcttcggt tatatcttga gtagttccaa tcatgttttt agtgcctagc gaatcggtat
     9721 aaatttttgc agttgttcta aagtttctta gttctccatc ttttctaatg attctgtagt
     9781 taatggttgg cagctctcct ttgtcaataa tgctttgatt tccattaacg attatgtgtt
     9841 tgtcatctgg atgtacaaat tctaacaaat tctcaattga tggattaaaa gactgaggtt
     9901 cacaacccaa taatcgaaat ttattatcag aataaataaa ttctaaagtc ttaaaattta
     9961 atatccaact accataatta cctaatattt ctgcttgttt actcgattcg tcatatattt
    10021 ttaatcgttt attatattca attaattctt cattgttttt tttgattttt tcagtatata
    10081 tttggtggtc agtaacatct tgagttgttc ccacaagtat attttctcca aaattatttt
    10141 gtattaaaat tccagttgca tgaaaaaatc taatttctcc gtcttttttg ataattctat
    10201 aagtgtatcc aggtaattct tttaactcta aaatattggc tgatttagtt ttaacaaaat
    10261 ctaaatcatc aggatgaatt gtttttaaat attcttcaag tgttggttca aattcttgag
    10321 gttcatagcc aagtaatcga taaaggttat ctgaaaattg ataactattt ctttctaaat
    10381 taacttccca acttccaaat ttaccaataa tttcagcaag tttttgagat tcgttaatca
    10441 ccattaaacg gtcgttggaa cttctcaaac taattaaatc tttgttaatt cttatataag
    10501 caacaacaat tagaaagagt gttattatga tgattagtaa agcataaaat ggagtgtaaa
    10561 aggaagtgtc tttaaatgat ttctctcttt tttcaagtaa ataatcttca agttttgaca
    10621 tttcgtccgt tttttttcga acattgtcca ttgctttttt gctgttggct ataaaatatt
    10681 tttggtattt ataatcgctt ttaatctctc ggtattcgtc aaaataggaa tatcgaattg
    10741 ctatgagtcg gtacaactct cttaaatttt tttgttgggt tttattttct tttgttagtc
    10801 ttttaattct aataaatgct ttatcaacat tttctctcga attattgaaa gggttaagaa
    10861 aagctttgtc tttgtataat aaataccctc taaaaccagt ttcggcatct tttagctccg
    10921 acattaaatt ttcaatgttt agttttacct catacgattt catcaccaag tctgttgatt
    10981 tgttaagttc aacaatatgt ttgtaagtga tagatgctgt aaaaaaaata ctagcaagag
    11041 atattacaaa agcaatattt agaaaaatcc ttgatttaaa ggtgtttatt attctaat
//
LOCUS       DAOOBH010000075        11046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_7522_length_11046_cov_5.884633, whole genome shotgun sequence.
ACCESSION   DAOOBH010000075 DAOOBH010000000
VERSION     DAOOBH010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7522_length_11046_cov_5.884633"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..555
                     /gene="aroQ"
                     /locus_tag="PK218_08150"
     CDS             142..555
                     /gene="aroQ"
                     /locus_tag="PK218_08150"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPW98518.1"
                     /translation="MKLIIINGPNLNLLGKREPELYGSQTFDEYYQALKKKYNTIELV
                     YYQSNIEGELIDKIQEVGFDFDGIILNAAAYTHTSVGIADAVKAITTPVIEVHISNTF
                     ARETFRHHSFLSSNVKGIIIGFGMKSYELALQSFL"
     gene            628..3084
                     /locus_tag="PK218_08155"
     CDS             628..3084
                     /locus_tag="PK218_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPW98519.1"
                     /translation="MKKTLLFALIVFAQFSFSQIRGVVKDAEGKPLPFVNIFEENTYN
                     STTTNDLGRFELSIKTQGSHVILFQYLGYKTGKRVVEVTSTPIEIEIVLQEENISLNE
                     VVVDKGINPADEIIRNAIKNKKYNSEKAARYTADFYSRGIFRVKNLPKTILGQKLDFF
                     DEIIDSTRSGILYLSETVSKVTFQKPDKLKEVIIASKVSGNDNGFSFNNAADANFDFY
                     DNYLEFDTNVISPIADNAFNYYKYKFEGSFFTENNQQINKIKVLPRRASEPVMSGYIY
                     IVDDTYAIYATDVAFTGKQIQNPALNTLSLKQNYSYNTKTKIWAKNTQTIDFDAGMLG
                     VNISGRFTYVFSNFEFPEKFEKRTFTAEVVRFEENANKKEDAFWNTIRPVPLTLEESA
                     DYVKKDALQEKKKSEVYLDSIDTKKNKFRPMDVLNGYTYNNSFKKWRITYDGPLMNTS
                     FNTVQGWNTSAGLSFFKRNEENRTYYQFGSNVAYGFSDEAYRPTAYFSAGLNNKTKSY
                     LSVYGGNAATQFNRDEPISKIVNTVSTLFFKSNFMKLYERNYIAASFSREIINGLNMS
                     LGLEYAERKPLYNTTDYTLIKSDDLYTSNNPFLPFDNTTSAIEKHNLAKLNIGARIKF
                     GQQYITRPDGKYNLGNDKYPTLLVGYEKGFSGNESNYNYDKIAVRVTQNLSLGNKGEL
                     AINTKAGKFFNADGISFVDYKHFNGNQTHFNQGDMTNSFNLLPYYSASTNDSYFETHL
                     EHNDKGYIMNKIPLLNKLQSKLMLGFKNLAIPERSPYQEYSVGLNNLGFGKFHILRVD
                     YIRSYQGGYLGDGVMFGLSF"
     gene            complement(3163..4473)
                     /gene="murA"
                     /locus_tag="PK218_08160"
     CDS             complement(3163..4473)
                     /gene="murA"
                     /locus_tag="PK218_08160"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPW98520.1"
                     /translation="MGTFKIEGGIQLKGDVQPQGAKNEALQILCAVLLTPEKVTINNI
                     PDIIDVNKLITLLGNLGVKIKKNGHGSLTFQADDVNVGYLETEAFKKEGGSLRGSIMI
                     VGPLLARFGKGYIPKPGGDKIGRRRLDTHFEGFINLGAKFRYEREDHFYGVESKGKLK
                     GAYMLLDEASVTGTANIVMAAVLAEGTTTIYNAACEPYLQQLCKMLNSMGANIKGVGS
                     NMLTIEGVDALGGCTHRILPDMIEIGSWIGLAAMTRSEITIKNVSWDNLGVIPSVFRK
                     LGITLEQKGDDIYIPAHTNGYKIKTDIDGSILTIADAPWPGFTPDLLSIILVVATQAD
                     GDVLIHQKMFESRLFFVDKLIDMGAKIMLCDPHRAVVMGHNFKSQLKATVMSSPDIRA
                     GISLLIAALSAKGTSTIQNIEQIDRGYERIDERLRALGANIVRV"
     gene            complement(4501..5193)
                     /locus_tag="PK218_08165"
     CDS             complement(4501..5193)
                     /locus_tag="PK218_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HPW98521.1"
                     /translation="MNQKYIKETANDVVHHLEYNAERPHLIIPEYGRHLQKLIDQATA
                     INDKIERNKAANYIIQVMGSLNPHLRDVLDFQHKLWDQLFIMSDFKLDVDSPYPIPTR
                     EVINLKPDRLEYPQKNPKYRFYGNNIKYMIDVANSWDDGEMKNALIRVIANHMKKSFL
                     SWNKDTVTDAVIFEHLFELSKGRINLVQSDEELINTNDLLRTNKKTSNKTNVQNNKTK
                     IKTKKPFQRKNN"
     gene            complement(5195..5479)
                     /locus_tag="PK218_08170"
     CDS             complement(5195..5479)
                     /locus_tag="PK218_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HPW98522.1"
                     /translation="MDKKTEEFYIRLREELNNTSTWPSEYLYKFIVPTDPKKVEEVEN
                     AFDNMGAVIKTSQSKTGKYTSLSVNVTMNNPDAVVEKYIEVSNIDGIISL"
     gene            complement(5611..6903)
                     /gene="eno"
                     /locus_tag="PK218_08175"
     CDS             complement(5611..6903)
                     /gene="eno"
                     /locus_tag="PK218_08175"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPW98523.1"
                     /translation="MSIIIKVHARQILDSRGNPTIEVDVVTENGVLGRAAVPSGASTG
                     EHEAVELRDGGKAYMGKGVLKAVENVNTKIAPELLGTSVFEQNHIDQLMIDLDGTTNK
                     SNLGANAILGVSLATAKAAANELGLPLYRYVGGVSANTLPVPMMNIINGGSHSDAPIA
                     FQEFMIMPVKAQNFTHAMQMGTEIFHNLKKVLHDRNLSTAVGDEGGFAPNLAGGTEDA
                     LDTIKLAVTNAGYTLGDDVMIALDCASAEFFVNGKYDYTKFEGTTGKVRTSNEQAEYL
                     AELTKKYPIISIEDGMDENDWEGWKYLTELVGDKVQLVGDDLFVTNVTRLSTGIEKGI
                     ANSILVKVNQIGTLTETIAAVNMAHNAGYTSVMSHRSGETEDNTIADLAVALNCGQIK
                     TGSASRSDRMAKYNQLLRIEEELGDTAYFPGIKAFKQK"
     gene            complement(7060..8181)
                     /gene="carA"
                     /locus_tag="PK218_08180"
     CDS             complement(7060..8181)
                     /gene="carA"
                     /locus_tag="PK218_08180"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPW98524.1"
                     /translation="MHYTTRQKAIILLADGTIFHGKSIGISGKTFGEVCFNTGMTGYQ
                     EIFTDPSYFGQIMVATNPHIGNYGVNSEETEADKIMISGLVCKNFSFTHSRPNSESNL
                     YDYFEKENLICISDVDTRALVSYIRDNGAQNAVICTDDTSIDALKKQLASVPNMKGLE
                     LASKVSTKEPYFFGNENATFKISALDLGIKRNILRNLANRNCYIKVFPFDASFEDLKS
                     FEPDGYFLSNGPGDPEPLENAQKLAKQIIDCNFPLFGICLGHQVIGLANGISTYKMFN
                     GHRGINHPVMNMITGKGEITSQNHGFAVNREELENHPDLEITHYHLNDNTVAGMRMKS
                     KNCFSVQYHPEASPGPHDSSYLFDDFITNIKKSKSLEKI"
     gene            complement(8350..8862)
                     /gene="rplQ"
                     /locus_tag="PK218_08185"
     CDS             complement(8350..8862)
                     /gene="rplQ"
                     /locus_tag="PK218_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPW98525.1"
                     /translation="MRHGKKFNHLSRQTGHRKSMLANMACSLIEHKRINTTVAKAKAL
                     KQFVEPLITKSKEDTTHNRRVVFAYLRNKYGVTELFREVAAKVGDRPGGYTRIIKVGN
                     RLGDNADMAMIELVDFNELYNGGKKEEKKAKSRRGGKGKKAETTAPVAETSAAEAPET
                     SSEETTNSAE"
     gene            complement(8901..9893)
                     /locus_tag="PK218_08190"
     CDS             complement(8901..9893)
                     /locus_tag="PK218_08190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPW98526.1"
                     /translation="MAIFNFQKPDKVIMIDSTDFEGKFEFRPLEPGYGLTVGNALRRV
                     LLSALEGYALTSVRIEGVEHEFSTISGVVEDVTEIVLNLKQVRFKRQIEEIDNETVSI
                     SISGKNQITAGDFQKFISGFQVLNPELVICNLDSKVNLNIELTIEKGRGYVPAEENKK
                     QNAAIGTIFIDSIYTPVKNVKYSIENFRVEQKTDYEKLVFEIKTDGSINPKDALTEAA
                     KVLIHHFMLFSDERITLEADEIAQTESYDEESLHMRQLLKTKLVDMDLSVRALNCLKA
                     AEVDTLGDLVSFNKNDLMKFRNFGKKSLTELDELVANKNLTFGMDLGKYKLDKE"
     gene            complement(9915..10520)
                     /gene="rpsD"
                     /locus_tag="PK218_08195"
     CDS             complement(9915..10520)
                     /gene="rpsD"
                     /locus_tag="PK218_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPW98527.1"
                     /translation="MARYTGPSTKIARKFGEAIFGEDKAFEKRNYPPGQHGMAKRRGK
                     KSEYAIQLMEKQKAKYSYGILEKQFRNLFEKASASKGVTGEILLQLCEARLDNVVFRM
                     GIAPTRRGARQIVSHRHITVNGEIVNIPSYHLKPGDKVAVREKSKSVEAIERSLANSS
                     HVYEWITWNNDTKEGTFVSVPARLQIPENIKEQLIVELYNK"
     gene            complement(10603..10986)
                     /gene="rpsK"
                     /locus_tag="PK218_08200"
     CDS             complement(10603..10986)
                     /gene="rpsK"
                     /locus_tag="PK218_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPW98528.1"
                     /translation="MAKTTAKKRKVIVESTGEAHINATFNNIIISLTNKKGEVISWSS
                     AGKMGFRGSKKNTPYAAQMAAEDCGKVALEAGLKKVKVYVKGPGNGRESAIRSIHNSG
                     IEVTEIIDVTPMPHNGCRPPKRRRV"
BASE COUNT         3421 a         1992 c         1774 g         3859 t
ORIGIN      
        1 aggaaatata gcaggctttt tagttatcaa caaggttatt aacaattaaa aagttttatt
       61 cttgaaatta ttttgattct aatttagatt aaaatgttgt tccttacgta cttttaatta
      121 tttttacttc aaatacactc tatgaaactc atcattatca acggtcctaa cttaaaccta
      181 cttggaaaac gcgaacctga attatacggc agtcaaactt ttgacgaata ttatcaagca
      241 cttaaaaaga agtataatac aattgagtta gtctattatc aaagtaatat tgaaggcgaa
      301 ttgattgata aaattcaaga agtgggtttt gattttgatg gtattatatt aaatgctgcc
      361 gcctacacac atacttctgt tggtattgct gatgctgtaa aagcaataac aactcctgtt
      421 atagaagtgc atatctcaaa tacttttgct agagaaacgt ttagacatca ttccttttta
      481 tcttcaaatg ttaaaggaat aataattggc tttggaatga aaagttatga attagcttta
      541 caatcgtttt tataattcat ttaaaaaaac aaatcctctg tacaaccatt ttatcaaaca
      601 aaacgtctat actgaaaaac ctaatccatg aaaaaaactt tactatttgc acttattgta
      661 tttgcccaat tttcgttctc tcaaattcgg ggcgttgtaa aagatgctga aggaaagcca
      721 ctgccgtttg taaacatatt tgaagagaac acatacaatt ctacaacaac caatgatttg
      781 ggaagatttg aattaagcat caaaacacaa ggaagtcatg taatcctttt tcaatatttg
      841 ggatacaaaa ctggcaaaag agtcgtggaa gttacatcta ctcctatcga aattgagatt
      901 gttctccaag aagaaaacat ctcattaaat gaagtggtgg tagataaagg aataaatcct
      961 gcggatgaaa tcattagaaa tgccattaaa aacaaaaaat acaacagcga aaaagccgca
     1021 agatatactg ccgattttta ttcgagaggt atttttagag taaaaaattt acccaaaact
     1081 atattgggac aaaagctcga tttttttgat gaaataattg actctacaag aagtggtatt
     1141 ttgtatttat ctgaaacggt atctaaagtt acttttcaaa aacctgataa attgaaagaa
     1201 gtaattatcg cttcaaaagt gagtggaaac gataatggct ttagttttaa caatgctgct
     1261 gatgccaatt ttgattttta tgacaactat ttagagtttg acaccaatgt tatttcgcca
     1321 attgctgaca atgcttttaa ttattacaaa tacaaatttg aaggttcgtt ctttaccgaa
     1381 aacaaccaac aaattaacaa aatcaaagta ttaccacgca gagcatcgga acctgtgatg
     1441 tcgggttaca tttatattgt ggacgatact tatgccattt atgcaaccga tgtagccttt
     1501 actggaaaac aaattcagaa tcctgcgttg aacacattga gcttaaaaca aaattatagc
     1561 tacaatacaa aaactaaaat ttgggcaaaa aacacccaaa ccatagactt tgacgcggga
     1621 atgcttggtg taaacatttc ggggagattt acctatgtgt tttcaaattt tgagtttcca
     1681 gaaaaatttg aaaagcgaac ctttactgcc gaagtagttc gatttgaaga aaacgccaat
     1741 aagaaagaag acgctttttg gaacaccatc agaccagtgc cattaacgct agaagaatct
     1801 gcagattatg taaagaaaga cgctttgcaa gaaaaaaaga aatcggaagt atatcttgat
     1861 tctattgata ccaaaaaaaa taaatttcgt ccaatggatg ttttgaatgg ttacacctat
     1921 aataattcat ttaaaaaatg gcgtatcact tatgatggtc cgctaatgaa tacttccttc
     1981 aacacggtgc aaggttggaa taccagtgct ggattatcct tctttaaaag aaacgaagaa
     2041 aacagaactt attaccaatt tggctccaat gttgcctatg gtttttctga tgaagcctat
     2101 aggccaactg catatttttc ggcaggatta aacaataaaa ccaaatctta tttatcggtt
     2161 tatggtggta atgcggctac tcaatttaac cgtgacgaac ctatttctaa aatagttaac
     2221 accgtgagta cactgttttt caaaagcaat tttatgaaac tttatgaacg caactatatt
     2281 gccgcaagtt tctctagaga aatcatcaac ggtttaaaca tgagtttggg tttagaatat
     2341 gccgaacgaa aaccattgta taataccacc gattatacac ttattaaatc ggacgattta
     2401 tatacctcaa acaatccttt tttgccgttt gacaatacaa catcggctat tgaaaagcat
     2461 aatctagcta agttgaacat tggtgcacgc atcaaatttg gtcagcaata cattactaga
     2521 ccagatggca aatacaattt gggcaacgac aaatacccaa cacttttagt aggctacgaa
     2581 aaaggatttt cgggcaatga aagtaactat aactacgata aaattgcagt tcgtgttact
     2641 caaaaccttt cgttgggcaa caaaggtgaa ttagcaataa acacaaaagc tggcaaattc
     2701 tttaatgccg atggaatttc gtttgtagat tacaaacatt ttaatggcaa tcaaacccat
     2761 tttaatcaag gcgacatgac caattcgttt aacttgttgc cttattactc tgctagcacg
     2821 aatgatagtt attttgaaac ccacctagag cataatgaca aaggatatat tatgaataaa
     2881 attcctttat taaacaaact gcaatccaaa ttgatgcttg gctttaaaaa ccttgctata
     2941 ccagagcgta gcccgtatca agaatattca gttggattga ataaccttgg ttttggaaag
     3001 ttccatatcc taagagttga ctatattcgt tcctatcaag gtggttatct tggcgatggt
     3061 gttatgtttg gtttaagttt ctagttaaaa aaagaaattc caaattccaa gcatttcgat
     3121 actttggaat ttggaatttt aattattgga atttttgtcg ggttataccc taacaatatt
     3181 ggctcccaaa gcacgaagtc tttcgtctat acgctcgtag cctctatcta tttgttcaat
     3241 attttgaata gtcgaagttc cttttgcaga tagtgccgca attaataacg agatacccgc
     3301 acgaatatct ggcgaactca taacggttgc ttttaattgc gatttaaaat tgtgccccat
     3361 aaccacggct ctatgcggat cgcaaagcat tattttggca cccatatcaa tcaatttatc
     3421 cacaaagaat aatctgcttt caaacatctt ttggtgaata agtacatcgc catctgcttg
     3481 agttgctact accaaaatga tgctcaataa gtcaggggta aaacctggcc atggcgcatc
     3541 ggcaatggta agaatagaac cgtcaatgtc agttttaatt ttatatccgt tggtatgagc
     3601 aggaatatag atatcgtctc ctttttgctc taaagtaatt cctaattttc taaacacgct
     3661 aggaataact cctagattgt cccaagatac atttttgatg gtaatctcac ttcgtgtcat
     3721 cgctgcaaga ccaatccacg aaccgatttc aatcatgtcg ggcaatattc tgtgcgtgca
     3781 gccaccaagc gcatcaacac cttcaatggt tagcatgttt gacccaacac ctttaatgtt
     3841 tgctcccata gagtttaaca ttttgcaaag ttgttgcaaa taaggttcgc aagcagcgtt
     3901 gtatattgtg gttgttcctt cggcaagcac ggcagccatt actatgtttg ccgttcctgt
     3961 tactgatgct tcgtcaagca acatataagc accttttagt ttgcctttag attcaacacc
     4021 atagaaatgg tcttctcttt cgtatctaaa cttagcgcca agattgataa acccttcaaa
     4081 gtgtgtatct aatcttcttc ttccaatttt atcaccgcct ggttttggga tgtatccttt
     4141 tccgaaacgg gctaacaatg gaccaacaat catgatagag ccacgaagac tgccgccttc
     4201 ttttttgaat gcctcggttt ctaagtaacc aacgtttaca tcatcggctt gaaaagttaa
     4261 tgaaccatga ccattttttt taattttcac accaaggttt cctagtagtg tgattaattt
     4321 attgacatca ataatatcag gaatattgtt gatggttact ttttctggtg ttaataatac
     4381 ggcacataat atttgtaacg cttcgttttt agcaccttgt ggctgaacat caccttttag
     4441 ttgaatgcca ccttctattt taaatgtacc catttgatat aaataagttt cggagtttaa
     4501 ttaattgttt ttacgttgga atggtttctt ggttttgatt ttagttttat tgttttgaac
     4561 attcgtttta ttagaagttt tcttgtttgt tctaagcaaa tcgttggtat ttataagttc
     4621 ttcatcgctt tgaactaagt ttattctacc ttttgagagt tcgaatagat gctcaaaaat
     4681 caccgcatcg gtaaccgtat ctttattcca gctaaggaat gattttttca tgtgattggc
     4741 aattactctt attaaagcat ttttcatttc cccatcatcc caactgtttg ctacatcaat
     4801 catgtattta atattgttcc cataaaatcg atatttagga ttcttttgag gatactccaa
     4861 acggtcaggt tttagattaa tcacttcgcg agtcggaatt ggatatggag aatcaacatc
     4921 taatttaaaa tcagacatga tgaacaattg atcccaaagc ttgtgttgaa aatcaagcac
     4981 atcacgaaga tgaggattta aactacccat aacttgaatg atataattgg ccgctttgtt
     5041 gcgctcaatt ttatcgttga ttgcagtggc ttgatcaatc agtttttgca aatgacgccc
     5101 atattctggt ataattaaat gtggtcgttc agcattatat tctagatgat gcaccacatc
     5161 atttgctgtt tcttttatat atttttgatt cattttaaag tgagattatg ccgtcaatgt
     5221 tagaaacttc aatgtatttt tcaaccacag catctggatt attcattgtt acattgactg
     5281 aaaggcttgt gtattttcct gttttggatt ggctggtttt aataactgca cccatattgt
     5341 cgaaagcatt ttctacttct tccacttttt tgggatcggt tggaacaata aatttataca
     5401 aatattcgct tggccaagtg gaagtattgt ttaattcttc tcttaatcgt atataaaatt
     5461 cttctgtttt cttatccata ataataaata taagcaaata taagatattg agttcacaaa
     5521 atataatttt aatagcagtg ggttttgaaa aacaaaaaac ctaacagata ttaataacct
     5581 gttaggcttg atagttaaaa tataataaga ctacttctgc ttaaaagctt ttattccagg
     5641 aaaataggct gtatcaccta attcttcttc aattcgaagt agttggttgt attttgccat
     5701 acggtcggaa cgagatgctg aacccgtttt gatttgacca cagtttaagg ctactgctaa
     5761 atcggcaatc gtattgtctt ctgtttctcc agaacgatgt gacatcacag aggtatatcc
     5821 tgcattgtgc gccatgttta ctgcagcaat agtttcggtt aatgttccta tttgatttac
     5881 ttttactaag attgaattgg caattccttt ttcaattccg gttgataaac gtgttacatt
     5941 agtaacaaac aaatcatctc caactaattg cactttatca ccaactaatt cggttaaata
     6001 tttccaacct tcccagtcgt tttcatccat accatcctcg atagaaatga ttgggtattt
     6061 tttagtcaat tcagctaaat attctgcttg ctcgtttgaa gttcttactt ttccagtagt
     6121 tccttcaaat tttgtgtagt cgtatttacc attaacaaaa aattctgctg aagcacaatc
     6181 tagagcaatc attacatcgt cgccaagtgt atatcctgca ttagttactg ctagtttgat
     6241 agtatctaat gcgtcttctg ttccaccagc taaattaggc gcaaaaccac cttcatcgcc
     6301 tactgccgta gaaagatttc tatcgtgtaa aacttttttt aggttatgaa aaatttcagt
     6361 tcccatttgc attgcgtgtg taaaattttg tgcttttaca ggcataatca tgaattcttg
     6421 aaatgctatt ggcgcatcgg aatgtgaacc accattgata atgttcatca ttggaacagg
     6481 caacgtatta gcagaaacac caccaacata acgatataat ggaagaccta attcatttgc
     6541 cgcagctttt gcagtagcca atgaaacgcc caaaatagca tttgcgccaa ggtttgattt
     6601 attggtagta ccgtccaaat caatcatcaa ttgatcgata tgattttgct cgaaaacaga
     6661 agttcctaat aattcaggcg caattttagt atttacattt tcaacagctt taagcacacc
     6721 ttttcccatg tatgcttttc ctccatcacg aagttcaacc gcttcgtgtt ctccggtgga
     6781 agcaccagaa ggaacagcag cacgtcctaa aacgccgttt tctgtcacta catctacttc
     6841 gatagttgga tttcctcttg aatctaaaat ttgtcttgcg tgtactttaa taataatact
     6901 catatttatg atattaagtt tttgatatta atttttagtc ctttgactgt gcttaggata
     6961 acaacaaata tatcctatct tttattatca atttaacaat agaaacgaat gttattaacg
     7021 caaacgtttt agtgttctat ttttaatttc tattgattat taaatttttt ctagcgattt
     7081 acttttttta atatttgtta tgaaatcatc aaaaagataa gacgaatcat gtggccctgg
     7141 actcgcttct ggatggtatt gtaccgaaaa acaatttttg gatttcattc tcataccagc
     7201 aacggtattg tcattcaaat gataatgtgt tatttctaaa tcaggatgat tttcaagttc
     7261 ttctctattg accgcaaaac cgtgattttg agaggtgatt tcaccttttc cagtaatcat
     7321 attcatcact ggatgattga ttcctcggtg accgttaaac attttatagg tagaaatacc
     7381 atttgccaaa ccgattactt gatgaccaag acatattcca aataaaggaa aattacagtc
     7441 aatgatttgc ttggctagtt tttgagcatt ttctaaaggt tcaggatcgc ctggaccatt
     7501 tgataaaaaa taaccatcag gttcgaatga ctttaaatct tcaaaggaag catcaaatgg
     7561 aaagacttta atatagcaat ttctattagc taaatttcga agtatgtttc tttttattcc
     7621 taaatctaaa gcagaaatct taaacgttgc gttttcattt ccaaaaaaat aaggttcttt
     7681 agtagaaact ttggatgcca actctaaacc tttcatattt ggaacgcttg ctaattgttt
     7741 ttttaaggca tctatggatg tatcgtcggt acaaatcaca gcattttgcg caccattatc
     7801 tctgatgtaa cttactaaag cacgagtatc aacatctgaa atacagatta ggttttcttt
     7861 ttcaaaataa tcataaagat tactttcaga atttggacga gaatgtgtaa aactgaaatt
     7921 tttgcaaacc aatccagata tcatgatttt atcagcttcg gtttcctcag aattcacgcc
     7981 atagtttccg atatgtggat ttgtagccac catgatttga ccaaaatagg aaggatcagt
     8041 aaaaatttct tggtaacctg tcatccctgt attgaaacaa acttctccaa aagttttccc
     8101 ggagattccg atagatttac cgtgaaaaat agtaccgtct gccaataaaa tgattgcttt
     8161 ttgtcttgtt gtgtagtgca tttgtgtagt tgtgtttagc ttcgctctgt tcggactttg
     8221 tcctcgggtt acaaatttat ttaattagaa ttgaaaaaga aaatagaatt atccaacttt
     8281 ataaacaaaa tgaataaaaa aaaaggataa acaaaatgtt tatcctttag tattaaaata
     8341 gtgctttcat tattcagcgc tattggttgt ttcttctgaa gaagtttctg gagcttctgc
     8401 agcggatgtc tcagcaactg gagcagtagt ttctgctttt ttaccttttc caccacgacg
     8461 gcttttagct tttttctctt ctttcttacc tccgttgtat agttcgttaa aatctacaag
     8521 ctcgatcatt gccatatcag cattatctcc caaacgattt cccactttga ttatacgagt
     8581 ataacctcct ggacgatcac caacttttgc tgcaacttct ctaaaaagtt cggttacacc
     8641 atatttattt ctaaggtaag caaacactac acgacggttg tgcgtagtat cttcttttga
     8701 ttttgttata agtggctcaa caaattgttt aagcgctttt gctttagcaa cagtagtgtt
     8761 aatacgtttg tgctcaatta aagaacaagc catattagct aacatagatt ttctatgtcc
     8821 agtctgtctg cttaagtgat tgaatttttt tccgtgtctc attgctatct aaatttaacc
     8881 tcgatgttta cgagatttaa ttattcttta tctaatttat attttcctaa atccattccg
     8941 aatgttaaat tcttgttggc aactaattca tctaattcag ttaatgattt tttaccaaaa
     9001 ttacggaact tcataaggtc gttcttgttg aaagaaacta aatctccaag agtatcaacc
     9061 tctgcagctt tcaaacaatt taaagctctt acagataaat ccatatcaac taattttgtt
     9121 ttaagcaatt gtctcatgtg caaagattct tcatcataag actcagtttg agcaatttca
     9181 tccgcttcta aagtaattct ctcatcggag aacaacataa agtggtgaat taaaactttt
     9241 gcagcttctg ttaatgcatc tttaggatta atagatccat cagttttgat ttcaaaaact
     9301 aatttttcgt aatctgtttt ttgctcaaca cggaagtttt caatagaata cttaacgttt
     9361 ttaacaggag tataaattga gtcaataaaa attgtcccaa ttgcagcatt ttgctttttg
     9421 ttttcttcgg caggaacgta tccacgacct ttctcaatag tcaactcaat atttaagtta
     9481 actttactat caagattaca aattactaat tctgggttta atacttgaaa tcctgaaatg
     9541 aatttttgaa aatcaccagc agttatttga tttttaccag aaattgagat agaaacagtt
     9601 tcgttatcaa tttcttcaat ctgacgtttg aaacgtactt gtttaagatt taaaacgatt
     9661 tctgttacat cttcaacaac tcctgagata gttgagaatt catgttctac tccttctatg
     9721 cgaaccgaag ttagcgcata accttctaaa gcagaaagta gaactcttct taatgcatta
     9781 ccaactgtca atccataacc aggttctaaa ggacgaaatt caaatttgcc ttcaaaatcg
     9841 gtcgaatcga tcatgataac tttatcgggt ttttgaaaat taaatattgc cataatttag
     9901 actaatgtca atttttattt gttgtataac tctacgatta attgttcttt gatgttttca
     9961 ggtatttgta accttgcagg tactgaaaca aaagtacctt ctttagtatc gttattccaa
    10021 gtaatccatt cgtacacatg acttgaattt gctaatgaac gttcaatagc ttcaactgat
    10081 tttgattttt cacgaacagc cactttgtcg cctggtttta agtggtaaga tggaatgttt
    10141 acgatttcac cattaacagt aatgtgtctg tgagaaacaa tttgtcttgc gcctcttcta
    10201 gttggtgcaa ttcccattct gaaaactaca ttgtctaatc tagcttcaca taattgtaaa
    10261 agtatttcac ctgttacacc tttagaagca gaagcttttt cgaataagtt tctgaattgt
    10321 ttttctagaa ttccgtagga gtacttcgct ttttgctttt ccatcaactg aattgcatat
    10381 tcagattttt tacctcttct ttttgccatc ccgtgttgac caggtggata atttcttttt
    10441 tcgaaggctt tgtcttctcc gaaaattgct tcgccaaatt tacgagcaat ttttgtgctt
    10501 ggaccagtat atcttgccat ttttaattta tttaagattg cgattatgaa ttcaggtcat
    10561 atccttcgat aatcttcaag caatcttggt tatactttat aattatactc tacgtctttt
    10621 tggtggacga caaccattat gtggcattgg tgtaacgtca ataatttcag ttacttcaat
    10681 tcctgagtta tgtattgatc tgatagcaga ttctcttcca ttaccaggac cttttacata
    10741 tacttttact tttttcaaac ccgcttctaa tgcaactttt ccacaatctt cagccgccat
    10801 ttgagcagca tatggggtat tctttttaga acctctaaag cccattttac cggcagatga
    10861 ccaagatata acttcacctt tcttatttgt taaagaaata atgatgttat tgaaggtagc
    10921 attaatatgt gcttcacccg tagattctac gataacttta cgtttttttg cagtcgtttt
    10981 agccatacta tttattattt agttgctttt ttcttgttag caacagtttt tcttttacct
    11041 tttctt
//
LOCUS       DAOOBH010000076        10999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_7560_length_10999_cov_4.858553, whole genome shotgun sequence.
ACCESSION   DAOOBH010000076 DAOOBH010000000
VERSION     DAOOBH010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10999
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7560_length_10999_cov_4.858553"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..973)
                     /gene="dcm"
                     /locus_tag="PK218_08205"
                     /pseudo
     CDS             complement(1..973)
                     /gene="dcm"
                     /locus_tag="PK218_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(1206..1397)
                     /locus_tag="PK218_08210"
     CDS             complement(1206..1397)
                     /locus_tag="PK218_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPW98529.1"
                     /translation="MNEGTIKFFNEAKGFGFITPADGSSDVFVHATSLNGQVRENDKV
                     TYDLEEGRKGIVAVNVTVV"
     gene            complement(1507..2031)
                     /locus_tag="PK218_08215"
     CDS             complement(1507..2031)
                     /locus_tag="PK218_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98530.1"
                     /translation="MNLDKLLELLMYAIPSLITGAVAYYMFDRHFKDQQNTRKWLVLR
                     ENQKQALPLRLQAYERLALLLERINPSKLLIRVAPIGDDKMDYQNLLIRHIEQEYEHN
                     LTQQIYISDDAWVMILTAKNTIIQNIRKVALDESIANADELREKILSNLFDNESASTV
                     ALAYLKSEVSEVLG"
     gene            2140..4470
                     /locus_tag="PK218_08220"
     CDS             2140..4470
                     /locus_tag="PK218_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPW98531.1"
                     /translation="MTKYISQLNEAQQQPVYQKDGPMIIIAGAGSGKTRVLTVRISYL
                     MSLGVDPFNILALTFTNKAAKEMKKRISEIVGSNEAKNLWMGTFHSVFAKILRIEAEK
                     LGYPSNFSIYDSQDSVRLISSIIKEMQLDKDIYKPKQVFSRISSYKNSLITVKAYFNN
                     PELQEADAMSKKPRLGDIYANYVERCFKAGAMDFDDLLLKTNELLNRFPDVLAKYQNR
                     FRYILVDEYQDTNHSQYLIVRALSDKFQNICVVGDDAQSIYAFRGANINNILNFQKDY
                     ENVKTYRLEQNYRSTKNIVEAANSIIDKNKTKLDKVVWTANDFGPKIKVHRSITDGEE
                     GRFVASEIFEQKMRNQMLNGQFAILYRTNAQSRAMEDALRKRDIPYRIYGGLSFYQRK
                     EIKDVLCYLRLVINPKDEEALIRVINYPARGIGNTTIEKLTVTANHYKRSIFEVMENI
                     DKIDLKINSGTKQKLEDFVMMIKSFQVINESQDAFFLADHVAKKTGLLQELKKDATPE
                     GIARLENIEELLNGIKDFTDGQIEVDGARGALSEFLEDVALATDLDKDTGDDDRVALM
                     TIHLAKGLEFPYVFIVGLEEDLFPSAMTLNSRSELEEERRLFYVALTRAEHQAYLTYA
                     QSRYRWGKLTDADPSRFIEEIKDEYLEYMNPDQHSNYRYKPTIDLDIFGDIDKSKLRS
                     VKPVAGTPPKQYGEEPSSSLNIRRLKPVSGNAPRIPNATDSKVAVGNIVMHERFGKGQ
                     ILNLEGIGGDKKAEIKFDVGGIKKLLLRFAKLDVIG"
     gene            4482..4676
                     /locus_tag="PK218_08225"
     CDS             4482..4676
                     /locus_tag="PK218_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98532.1"
                     /translation="MNTFLKNINWKIVLYWTIFMSFFNVFLLPYYDGEAFLAKKVILG
                     IIVWFLTGIIFAKIIQSRSS"
     gene            4741..5361
                     /locus_tag="PK218_08230"
     CDS             4741..5361
                     /locus_tag="PK218_08230"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPW98533.1"
                     /translation="MAEFIKIYEDKPSEAAIKKVVDVLKNGGLIIYPTDTVYGLGCDI
                     TNSRALEKIAKIKGIKIEKANFSFVCSDLSNISDYVRQIDTSTFKILKRALPGPYTFI
                     LPGNNDLPREFRKKKTVGIRVPANNIALEIVKLLGNPIVSTSIHDDDEVLEYSTDPEL
                     IFEKWQNIVDLVIDGGYGDNTPSTIIDLSGYEPEIIREGKGDIDIF"
     gene            complement(5358..6410)
                     /locus_tag="PK218_08235"
     CDS             complement(5358..6410)
                     /locus_tag="PK218_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HPW98534.1"
                     /translation="MKHNYILPLFLLFQILFLKIIAFFPEIVERFYSNGIYPIISKAL
                     RTILGPIPFSVGDILYGILIVYCIYWLYKNWKTSWKQKGLAILSFLSVFYFLFHFFWA
                     MNYYREPLFEKMNIRKEYSDNDLIVFTEKLIQKTNEIQFEITKNKNNKVVNPYTQEVV
                     FDLTENGYDNLTKQYPFFKYSIPSRKKSLFSLPLTYMGFGGYLNPFTNEAQVNYKLPM
                     YGFPAVVTHEMAHQMGYGSESECNFIGFLACIKNDDLYFQYAAYSNALRYCLSNIAQK
                     NEATFDQLKKKINRGVLKNYKESEVFWEQYDTIIDKGFHAFYDQYLKANQQQEGIESY
                     SKFVDLLINYYRNRSF"
     gene            6503..7969
                     /locus_tag="PK218_08240"
     CDS             6503..7969
                     /locus_tag="PK218_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPW98535.1"
                     /translation="MSQEIRNLEPKVLWNKFADLNEVPRPSKKEERVIEFMKNFGNNL
                     GLETFEDEIRNVIIRKPATPGMENRKTIVLQGHLDMVHQKNNDTDFDFLTQGIQMYVD
                     GDWVRAKGTTLGADNGLGVAAIMAILESKDIPHPAIDALFTIDEETGMTGALNLKGGI
                     LKGEILLNLDTEEDDEIDIGCAGGIDVTANRTYNEEETPEGSVGYTITVKGLNGGHSG
                     MDIHKGLGNANKIMNRLLFDGFENFGLQVAEISGGSLRNAIPRESVAKVIVAGMYDEA
                     FVFDMQEIINDIKAEYKTMEPKLTVEIVKSDLPSKVMDLGVQEGLLRSIYAAHNGVYR
                     MSPDMADLVETSNNIARVVVANGEISIQNLTRSSVESSKMDLANSLRSAYELFGCEVT
                     FGGSYPGWTPNVNSEILDVLVPIYEKQNNGEKPSVVACHAGLECGILGTNYPGMDMIS
                     FGPTIHGAHSPDERASIKSSQKFWKFLLEILANIPEKK"
     gene            8079..8672
                     /locus_tag="PK218_08245"
     CDS             8079..8672
                     /locus_tag="PK218_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98536.1"
                     /translation="MKKTAFLLMIMLCANLSFSQKKKTTAKKTTTEKTSSIKVDNLLA
                     EVKGKTFQVTITEKGKPTDALIVKELNDNLKPTDIKLSSFTASGTKLYLLQWTEKSNT
                     KTDVKTEDITTVFSVIYEIATKKQVFSNFEKTSNIVEKVFLDKNKNASETQEKIRREG
                     FVLTLNADGTVSQKNKTQENKWVYDAAKVDFVDAKKK"
     gene            complement(8797..9795)
                     /gene="lpxD"
                     /locus_tag="PK218_08250"
     CDS             complement(8797..9795)
                     /gene="lpxD"
                     /locus_tag="PK218_08250"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPW98537.1"
                     /translation="MKSFSVEYISNILGGKITGTTTNKITAPEQLENATETEISFIGN
                     KKYEKYWKNSKASVAVVNLDISIEPGENRVFIKVKNADLAMSQVLELFAPPTPFFKVN
                     IHPTAVIDDTATIGTGTKIGAGCYVGPNVTIAENVIIYPNVTILDESSVGKNTVIWSG
                     TVIRERCHVGAFCILHPNCTIGADGFGFRPCAEKGLVKIPQIGNVIIGNGVEIGANSC
                     VDRGKFSSTVLGDGCKIDNLVQIGHNSKLGKCCIMAGNSGLAGSVTLGNGVIIGGSAS
                     IKDHTSIGDGAIIGAGSGVAADVEAGKTMLGYPAVEARDALKQWAILKRLANNQNK"
     gene            complement(9943..>10999)
                     /gene="prfB"
                     /locus_tag="PK218_08255"
     CDS             complement(join(9943..10974,10976..>10999))
                     /gene="prfB"
                     /locus_tag="PK218_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986097.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPW98538.1"
                     /translation="LGALRRYLDIDAKLIEIANEEEKTYAPDFWNNAKEAEIIVKNLR
                     SKKQWVEDFEKANNLLEELQITYEFVKEGDASEEELDSLYETTNTFIENIEFKNMLSD
                     EGDNLSAVLQITAGAGGTESCDWASMLMRMYLMWAERQGYKIKELNFQEGDVAGIKTV
                     TLEIEGDFAFGYLKGENGVHRLVRISPFDSNAKRHTSFASVYVYPLVDDTIEIEINPA
                     DIEITTARSSGAGGQNVNKVETKVQLVHKPTGIQIQCSETRSQHDNRSRALQMLKSQL
                     YEIELKKQQEQRSDIEAGKMKIEWGSQIRNYVMQPYKLVKDVRTGKETSDVDGVMNGE
                     IDEFLKAYLMMMGQKEE"
BASE COUNT         3786 a         1970 c         1790 g         3453 t
ORIGIN      
        1 gttcttcata gtttatacaa taattactta atacagcact tttatccatt gaatttgaaa
       61 cgtaaggaag cagttttaaa attactgctt taactaaatt cacaggtaca gcattaccag
      121 cttgtctttt agcttgccca tcagaaacta caattttgta gctgtctgag aatccttgta
      181 atcgaaacat ttctcttggt gttaatcttc tttcaccatt tacaagtaaa taattgtgcg
      241 aagcaccaga gcgtaaggca cacgaataag gataagaaca gatgtttcct gacttgtttt
      301 cgtgccaaat agaaagatta tatgcagatt tatgcttgct ttttcttttt tctaaaatca
      361 tttcagaagc ataatattta gcatcaactt tcttttgaag aatttcagat aatggtttaa
      421 aaggtcttat tggagaagga aaattaaata aaatgggttc tttatgtcca acaattatta
      481 tacgctctct tttttgagga agaccataat caagtgcatt taatacagca aatctaacac
      541 tataacctaa atctttttcg agaacattta caatagtttt tagtgtattt ccaccatcat
      601 gtcctaaaag ttgtttaaca ttttctaata caaatgcttt tggtttttta tttttttaaa
      661 ttctagcaat atcaaaaaac aacgtacctc ttacatcatc aaatcctttc ctcttaccaa
      721 ttatgctaaa tgattgacaa ggaaacccag caaataaaat atcatggtct ggaatatctt
      781 tttcgtcaat ttttgttata tcgccaaatg gtctttctcc aaaattagct tcataactgt
      841 cttgacaata agtgtctata tccgatgaaa aaacacactc aggatttata ttgttttcaa
      901 tacaggcttc atccattgct gttctaaatc cgcctattct acaaaataaa tcaataaatt
      961 ttatattttc cattttctca aactttaatt caaaaaataa tattttaatt tatcattaaa
     1021 tagttcacac taattactta ttgacatctt ttcaacccta tatctcattc agatagtctc
     1081 caacctcgga gcatcttaat cccaaaaaaa tccttaatca tctttaatgg taaaaaaaac
     1141 aaacccccaa aaaataacct tctcatttga gaaggttatt actttgtatg gtaaaagata
     1201 agtagttata ctaccgttac atttactgca acgatacctt ttctaccttc ttcaaggtca
     1261 taggttactt tatcattttc acgaacttga ccattaaggc tagttgcgtg aacaaatacg
     1321 tcactacttc catcagctgg agtaataaat ccgaaacctt tggcttcgtt aaaaaatttg
     1381 attgttcctt cgttcattgt aatatttatt aaaatgcaaa tatagattta aattattgat
     1441 ttgcaacagg atatttaaaa acttcacaaa aaagaagtat attttcacta tagtggtgat
     1501 tatgagctat cctaaaacct cgctaacctc cgatttcaga taggctaaag caacagtact
     1561 agccgattcg ttatcaaata aattactcaa tattttttct ctcaattcat ctgcattagc
     1621 gatgctttca tccaaagcaa cctttctaat gttttgaata atagtattct tcgccgtcaa
     1681 aatcatcacc caagcatcat ccgaaatata aatttgttgc gtcaaattgt gttcgtattc
     1741 ttgctcaata tgacgaatca gcaagttctg ataatccatt ttatcatcgc caataggtgc
     1801 tacacgaatt aataatttag aaggattaat gcgttccaat aataatgcca atcgctcata
     1861 agcttgcaat cgtaaaggca aagcttgctt ttgattttct ctaagaacca accattttcg
     1921 agtattttgt tggtctttaa agtgtctgtc aaacatataa taggctacag caccagtaat
     1981 taaagatgga atagcataca taagtaactc taataactta tcaagattca tgatttttga
     2041 tttatatttt aattgttcac aaatataata gaaatactag taaaaattat tgttaatcag
     2101 gagcagtaat attaaatttg aactttatca attgctatga ttactaaata catttcgcag
     2161 cttaacgaag cccaacaaca acctgtttat caaaaagatg gtccaatgat tatcattgct
     2221 ggtgcaggtt ccggaaaaac acgtgtgctc accgttcgca tttcctacct gatgagctta
     2281 ggcgttgacc cattcaatat tttggcgtta acgtttacca acaaagccgc caaagaaatg
     2341 aaaaaacgta tttcagaaat agtaggaagc aatgaagcca aaaacctttg gatgggaaca
     2401 tttcattccg tttttgccaa aatcctacga atagaagcag aaaaacttgg ctatccatcc
     2461 aatttttcca tctatgattc ccaagattcc gttcgattaa tttcttctat aatcaaagaa
     2521 atgcaattag acaaagacat ttacaagcca aaacaagtct ttagtagaat ttcatcttat
     2581 aaaaactcct taattaccgt aaaagcttat ttcaacaatc cagagttgca agaagccgat
     2641 gctatgagca aaaaaccgcg cttaggagat atttacgcca actatgtaga acgttgtttc
     2701 aaagctggtg cgatggattt tgacgatttg ttattaaaaa caaatgaatt actaaaccgt
     2761 tttcccgatg tcttggcaaa atatcaaaac cgttttcgat acattttggt cgatgagtac
     2821 caagatacca accattcgca gtatttaata gtacgagcgt tgagcgataa atttcagaat
     2881 atatgtgtcg taggagatga tgcgcagagt atttatgcct ttcgtggtgc caacatcaac
     2941 aacattttaa acttccaaaa agattatgaa aatgtaaaaa cgtatcgatt agaacaaaat
     3001 taccgttcta ccaaaaacat agtcgaagcc gccaattcca ttatcgataa gaataaaacc
     3061 aaactagaca aagtggtttg gacagccaat gattttggac ccaaaataaa agtgcaccgt
     3121 agtattaccg atggtgaaga agggcgtttt gtagccagcg aaatctttga acaaaagatg
     3181 cgcaaccaga tgctcaatgg gcaatttgcc attttatata gaaccaatgc acaatcacgt
     3241 gctatggaag atgcgctgcg aaaaagagat attccctacc gaatttatgg cgggttatca
     3301 ttctatcagc gcaaggaaat caaagacgta ttgtgttatt tgcgattagt catcaaccca
     3361 aaagatgaag aagcgttaat ccgtgttatc aattatccag cacgtggaat cggaaatact
     3421 accatcgaaa aactaaccgt taccgccaat cattacaagc gttccatctt tgaagtgatg
     3481 gaaaatattg ataagattga cctcaaaata aactccggaa ccaagcaaaa gctagaagac
     3541 tttgtcatga tgattaaaag ttttcaagtc atcaacgaaa gccaagatgc ttttttccta
     3601 gccgaccatg tggcaaagaa aacaggttta ttgcaagaac tcaaaaaaga tgccactccc
     3661 gaaggaatag caagacttga aaacatagaa gaattgctta acggtatcaa agatttcacc
     3721 gacggacaaa tagaagtaga tggcgcacgt ggtgctttat ccgaattctt agaagacgta
     3781 gccttagcaa ccgatttaga taaagacacc ggcgatgacg atagagtagc actaatgaca
     3841 atacatttgg caaaaggttt agaatttccc tatgtattta tcgttggtct agaagaagat
     3901 ttattcccaa gtgctatgac cttaaactct cgttccgaac tagaagaaga acgtcgtttg
     3961 ttctatgttg ccttaacgcg tgccgaacat caggcgtatt taacctatgc acaatcgcgc
     4021 taccgttggg gaaaactaac agatgccgat ccatctcgat ttatagaaga aattaaagat
     4081 gaatatttgg aatacatgaa tccagaccag cacagcaatt accgttacaa accaaccatc
     4141 gatttggata tttttggcga tattgataaa tccaaacttc gctctgtaaa accagtggca
     4201 ggaacaccac caaaacaata tggcgaagaa ccttcatcat ccctaaacat tagaagatta
     4261 aaacccgttt caggcaatgc gccaagaata ccaaatgcaa cagatagcaa agtagcggtg
     4321 ggcaatatcg taatgcacga acgcttcgga aaaggacaaa tcctaaacct agaaggcatt
     4381 ggcggcgata aaaaggccga aataaaattt gacgtcggcg gaattaaaaa gctattgttg
     4441 cgtttcgcaa aattggatgt aataggataa actaattaga tatgaatact tttttaaaaa
     4501 atatcaactg gaaaattgtt ctttattgga cgatattcat gtcttttttc aatgtttttt
     4561 tattacctta ctatgatggc gaagcatttt tagccaaaaa agtgatttta ggaattatag
     4621 tttggtttct aacaggcatt atatttgcaa aaatcattca atcaagaagt agttaacatt
     4681 aaacccttaa attacattaa actcttaaac catattaaac ccttaaacaa tctttaaaaa
     4741 atggctgaat tcatcaaaat atacgaagac aaacccagcg aagctgccat taaaaaagta
     4801 gtggacgttt tgaaaaatgg cggattgatt atttatccaa ccgataccgt atatggttta
     4861 ggttgcgata ttaccaattc aagagcttta gaaaaaatcg caaaaatcaa aggtattaaa
     4921 atagaaaaag ccaatttctc ttttgtgtgt agcgatttga gtaatatttc cgattatgtg
     4981 cgtcaaattg atacatcaac cttcaaaatc ttaaaacgcg ctttgccagg accatatacc
     5041 ttcattcttc caggaaataa tgatttgcct agagagttta gaaagaaaaa aaccgttggt
     5101 attcgtgttc ctgccaataa tattgcactc gaaatagtaa aactactagg aaacccaata
     5161 gtgtcaacat ctattcatga tgatgatgag gttttagaat attccaccga tcccgaattg
     5221 atttttgaga aatggcaaaa tatagtcgat ttggttattg acggtggtta tggcgacaat
     5281 acaccatcaa ctattatcga tttatcaggt tacgaacccg aaatcatccg agaaggaaaa
     5341 ggcgatatcg atatatttta aaagcttctg tttctataat agttaatcag taaatccaca
     5401 aatttactat agctttcaat gccttcttgt tgttgattcg cttttagata ttggtcataa
     5461 aaagcatgaa accctttgtc gattatagta tcatattgtt cccaaaaaac ttcactttct
     5521 ttatagtttt ttaaaactcc tcggtttatt tttttcttta gttgatcaaa ggttgcttca
     5581 tttttttgcg caatgtttga caaacaatag cgcaaagcat tactataggc agcatattga
     5641 aagtacaaat catcattttt aatgcaagcc aaaaagccta taaagttaca ttcactttca
     5701 ctgccatagc ccatttgatg cgccatttca tgtgtaacca cggcaggaaa tccatacatt
     5761 ggtaatttat aattcacctg tgcttcatta gtaaagggat tcaaataacc accaaaaccc
     5821 atgtaagtca atggcaagct aaacaacgat ttttttctac ttggaattga atacttaaaa
     5881 aacggatatt gttttgtcaa attatcataa ccattctcag ttaaatcaaa aacaacttct
     5941 tgcgtataag gatttactac tttattgttt ttattcttcg ttatttcaaa ttgaatttca
     6001 ttcgttttct gaatcagttt ttcagtaaaa acaattaaat cattatccga atattctttt
     6061 cgaatattca ttttctcaaa aagaggttcc cgataataat tcatcgccca aaaaaaatga
     6121 aacaaaaaat agaatacaga aagaaaactc aaaatagcta aacctttttg tttccacgat
     6181 gttttccaat ttttataaag ccagtaaatg caatatacta tcaaaattcc atataaaata
     6241 tcaccaaccg aaaaaggaat aggtcctaaa atagttctca aagcttttga aataatcgga
     6301 taaattccat tagaataaaa tcgttctaca atttcaggaa aaaaggcaat tattttcaag
     6361 aaaaggatct gaaacaaaag aaaaagcgga agaatgtagt tgtgtttcaa ttttgacatt
     6421 taaaatgtaa atataatcac aaaactattt gaaagcattg taaactttgt aactttgggt
     6481 ttttaacatt caaatatatt acatgagcca agaaattaga aacctagaac caaaagttct
     6541 ttggaataaa tttgccgatt taaatgaagt gccacgtcca tccaaaaaag aagaacgtgt
     6601 aatcgagttt atgaaaaact tcggaaataa tctaggatta gaaacttttg aagacgaaat
     6661 tagaaacgta atcatccgaa aaccagcaac tccaggaatg gaaaatcgca agacgattgt
     6721 actgcaaggt catttggata tggtacacca aaagaataat gataccgatt ttgatttcct
     6781 aacacaaggc attcaaatgt atgtcgatgg cgactgggtt cgtgctaaag gaacaactct
     6841 tggtgctgac aatgggttag gagtagcggc tatcatggca attttagaaa gcaaagatat
     6901 tccacatcct gcgattgatg cacttttcac tattgatgaa gaaaccggaa tgacaggtgc
     6961 gttgaaccta aaaggcggta ttttgaaagg tgaaattctt ttaaatttag ataccgaaga
     7021 agatgatgaa attgatattg gttgtgcagg aggaattgat gttacggcaa acagaaccta
     7081 taacgaagaa gaaacacctg aaggttctgt aggttatacc atcaccgtaa aaggcttaaa
     7141 cggtggacat tccggaatgg atattcacaa aggtttaggt aatgccaata agattatgaa
     7201 ccgtttatta tttgacggat ttgagaattt tggacttcaa gttgctgaaa ttagtggagg
     7261 aagtttgcgc aatgcgatac caagagaaag cgttgccaaa gtgattgtag ctggtatgta
     7321 tgatgaggct tttgtgtttg atatgcaaga aatcatcaac gatatcaaag ctgaatacaa
     7381 gacgatggaa cctaagttaa ctgtcgaaat tgtaaaaagc gatttaccaa gtaaagtgat
     7441 ggatttaggt gttcaagaag ggttgttacg ttctatttat gcagcacaca atggcgttta
     7501 cagaatgtcg cctgatatgg cggatttggt agagacttct aataatatcg ctagagtagt
     7561 ggttgctaat ggtgaaatct ctattcaaaa cttaacccgt tcctcagtag aaagttctaa
     7621 aatggatttg gcaaatagcc tacgttctgc ttatgaatta tttggttgcg aagttacttt
     7681 tggcggaagc tacccaggtt ggacaccaaa tgtgaactct gaaatattgg atgttttagt
     7741 gccaatctat gaaaaacaaa acaatggcga aaaaccaagt gttgtagcct gtcacgcagg
     7801 tttggaatgt ggaattcttg gaacaaatta tccaggtatg gacatgattt cttttggacc
     7861 aaccattcat ggtgcacata gtccggatga aagagcttct attaaatcgt cacaaaaatt
     7921 ctggaaattt ttattggaaa tattagcaaa tattcctgaa aagaaataaa aagaagaata
     7981 tagaaattaa atcctcgctt atcactaagc gaggtttttt atttaacatt ggtcaaatca
     8041 tttattttgt atattggcat ttcaaaaaca atactacaat gaaaaaaaca gctttccttt
     8101 tgatgattat gttatgtgca aatttgagct tttctcaaaa gaaaaaaaca actgcaaaaa
     8161 agacaacaac cgaaaaaacg tcttcaataa aagtggataa cctactagct gaagtaaaag
     8221 ggaaaacgtt tcaagttacc attactgaaa aaggaaaacc aacagatgct ttaattgtaa
     8281 aagagctaaa tgataattta aagccaacag acattaaatt atcatccttt acagcaagcg
     8341 gaacaaaatt gtatttgttg cagtggactg aaaaatcaaa tactaaaaca gatgtaaaaa
     8401 cagaggatat tactacagtt ttttcggtta tttatgaaat tgcaactaaa aaacaagtct
     8461 tttctaattt tgaaaaaacg agtaatattg tagaaaaagt atttttagat aaaaataaaa
     8521 acgcttcaga aacgcaggag aaaataagac gtgaaggatt tgtattaaca ctaaacgctg
     8581 atggaactgt ttctcaaaag aataaaacgc aagaaaataa atgggtttat gatgctgcca
     8641 aagttgactt tgttgatgct aaaaagaaat agttttaaag attagcaaac tgttaaaaaa
     8701 acaaaagtcg atctggagcg atcgactaat gaaatgaatt gtaacatcct ttcggaatac
     8761 tcatagcaaa gatatatttt ttttttgaat actgaattat ttattttgat tatttgctag
     8821 tctttttaaa attgcccatt gtttaagagc atctcgagct tcaaccgctg ggtaacccaa
     8881 cattgtttta cctgcttcaa catcagcagc aacgccagaa cctgcaccaa ttatagcacc
     8941 atctccaatt gatgtgtggt cttttatcga agcacttccg ccaataatta ctccatttcc
     9001 aagagtgaca gaacctgcta agccagaatt tcctgccatt atacaacatt ttccaagttt
     9061 agagttgtgt cctatttgaa ccaaattgtc aattttacaa ccatctccaa gaactgtaga
     9121 actgaatttc cctctatcaa cacatgaatt ggctccaatt tctacaccat ttccaatgat
     9181 tacgtttcca atttgtggaa ttttaaccaa tcctttttca gcacaaggac ggaaaccaaa
     9241 accatcggca cctatagtac aatttggatg caaaatgcaa aaagcaccaa catgacaacg
     9301 ctcacgaata acagttcctg accaaatcac ggtgtttttt ccaaccgaag attcgtcaag
     9361 aattgtaacg ttagggtaaa taataacgtt ttcagcaatg gtaacatttg gtccaacata
     9421 acaaccagct ccaatcttcg ttccagttcc aatagtagcc gtgtcatcta ttactgctgt
     9481 tgggtgaata ttgactttga aaaaaggagt tggtggcgca aacaattcta gtacttgcga
     9541 catggccaaa tcagcatttt taacttttat aaacacacga ttttcacctg gttcaataga
     9601 aatatctaga ttaactactg ctacagaagc tttagaattt ttccaatact tttcgtattt
     9661 tttatttcct ataaaagaaa tttcagtttc agtcgcattt tctaattgtt caggtgcagt
     9721 aattttattt gttgtagttc ccgtaatttt accacccaaa atgttactta tatattcaac
     9781 tgaaaaggat ttcatgtaat taatttataa ttatcttcaa attaagcgaa atccttttaa
     9841 taaacctatt tagtaggatt ttttagtgta agttaaaatg ataaattttc acctttataa
     9901 ttacaaaaat ttactatcga atgtcagact gctaactgaa aactactcct ctttttgtcc
     9961 catcatcatt aaataggctt ttaagaattc gtctatttcg ccattcataa caccatcaac
    10021 atcgctggtt tcttttccgg tgcgaacgtc ttttacaagt ttgtagggtt gcatcacata
    10081 atttctaatt tgtgaacccc attcaatctt catttttccg gcttcaatat cgctgcgttg
    10141 ttcttgctgt ttctttaatt ctatttcata taattgcgat ttcagcattt gtaaagcacg
    10201 agaacgatta tcgtgttgcg aacgagtttc tgaacattga atctgtatgc cagtaggttt
    10261 gtgaaccaat tgtactttag tttctacttt gtttacattt tgaccaccag caccactaga
    10321 acgcgcagtg gtaatttcaa tatcagcagg attgatttca atttcaatgg tgtcatcaac
    10381 tagtggataa acatatactg atgcaaaaga agtatggcgc ttggcattac tatcaaatgg
    10441 tgaaatacgc actaagcgat gtactccgtt ttctcctttt aaatagccaa aagcaaaatc
    10501 gccttcaatt tctagagtta cggtttttat tccagcaaca tcgccttcct gaaagttcaa
    10561 ttcttttatt ttataccctt gacgttctgc ccacattaaa tacatgcgca tcagcatact
    10621 tgcccaatcg caactttccg ttcctcctgc gccagcagta atttgcaaca cagcacttag
    10681 attatcccct tcatcagaaa gcatgttttt aaactcgatg ttttcgatga aagtatttgt
    10741 agtttcatat aaagaatcta attcttcctc agaagcgtcg ccttctttta caaattcgta
    10801 ggtaatttga agttcttcca acagattgtt ggctttctca aaatcttcta cccattgttt
    10861 tttagaacgc aagtttttta caatgatttc ggcttctttg gcattgttcc aaaaatcggg
    10921 tgcataggtt ttctcttctt cgttggcaat ttctataagc ttggcatcaa tgtcaaagat
    10981 acctcctcaa cgcaccaag
//
LOCUS       DAOOBH010000077        10884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_7648_length_10884_cov_4.832210, whole genome shotgun sequence.
ACCESSION   DAOOBH010000077 DAOOBH010000000
VERSION     DAOOBH010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10884
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7648_length_10884_cov_4.832210"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1101)
                     /gene="gcvT"
                     /locus_tag="PK218_08260"
     CDS             complement(19..1101)
                     /gene="gcvT"
                     /locus_tag="PK218_08260"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPW98539.1"
                     /translation="MKNTALTNVHEQLGAKLVPFAGYNMPVQYEGVNIEHETVRNGVG
                     VFDVSHMGEFLLSGENALALIQKVTSNDASVLEIGRAQYSCLPNNDGGIVDDLIIYRI
                     KEHQYLLVVNASNIEKDWNWISSHNDLDVEMRDLSEDYSLLAIQGPKAVEAMQSLTSV
                     DLSAIKYYHFEVADFAGIEHVIISATGYTGSGGFEIYCKNSEVEQVWNKVFEAGASYG
                     IKPIGLAARDTLRLEMGFCLYGNDINDTTSPLEAGLGWITKFTKEFVNSENLKKQKEA
                     GVTRKLVGFELIDRGIPRHDYEIVDADGNNIGIVTSGTQSPSLGIAIGMGYVKTEFAG
                     VDSEIYIKIRNKDIKAKVVKLPFYKK"
     gene            complement(1152..2438)
                     /gene="fahA"
                     /locus_tag="PK218_08265"
     CDS             complement(1152..2438)
                     /gene="fahA"
                     /locus_tag="PK218_08265"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HPW98540.1"
                     /translation="MPINTNDPSRKSWLDVPDNSDFPIQNIPFGVFLTKDDVITIGTR
                     IGNYAVDLGALQQLHYFDGIELTDDMFMQDTLNDFISDGKKTWRMVRNRIGDIFDINN
                     PKLRDNPKHRDIVIFNMDEVEMQLPVLIGDYTDFYSSKEHATNVGKMFRDPENALLPN
                     WLHIPVGYHGRSSTIIPSGIPVHRPMGQTLPAGETEPVFGSSRLVDFELETAFITTDA
                     NVMGENIPVNEAEDYIFGMVLLNDWSARDIQKWEYVPLGPFLAKNFASSISPWIVTMD
                     ALEPFRVKGPNQEPKPLPYLQQKGKNAFDINLEVYIDTEKSNEPTLLSKSNFKYMYWS
                     MAQQLAHHTSNGCRINSGDMMGSGTISGPTPDSYGSMLELTWGGKNPITLNDGTERKF
                     IQDNDTVIMKGFCKNNSVRIGFGEVSTKLLPPYNRK"
     gene            2549..3823
                     /gene="glyA"
                     /locus_tag="PK218_08270"
     CDS             2549..3823
                     /gene="glyA"
                     /locus_tag="PK218_08270"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPW98541.1"
                     /translation="MQRDEQIFDLILDEQERQIHGLELIASENFVSDQVMEAAGSVLT
                     NKYAEGYPGKRYYGGCEVVDVIEQIAIDRAKTLFGAEYVNVQPHSGSQANTAVYAACL
                     KPGDKILGFDLSHGGHLTHGSPVNFSGKLYNPVFYGVDQETGVLNYDKIQEIATKEQP
                     KMIIAGASAYSRDMDFARFRVIADSVGALLLADISHPAGLIAKGLMNDPIPHCHIVTT
                     TTHKTLRGPRGGMIMMGKDFPNPWGLTTPKGEIRMMSHVLDMSVFPGNQGGPLEHIIA
                     AKAVAFGEALSDEFFTYQMQVQKNAKAMAEAFVKRGYNIISGGTDNHMMLIDLRNKNI
                     SGKEAENALVKAEITVNKNMVPFDDKSPFVTSGIRVGTPAVTTRGLMEADMETIVDFI
                     DRVIMNHTNEELLESIADEVNEMMSERAIFVY"
     gene            3986..5026
                     /gene="guaC"
                     /locus_tag="PK218_08275"
     CDS             3986..5026
                     /gene="guaC"
                     /locus_tag="PK218_08275"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HPW98542.1"
                     /translation="MRIETDLKLGFKDVMIRPKRSTLKSRAEVSLERTFKFMHSTYEW
                     KGVPIMAANMDTVGTFEMALALAQKDIFTAIHKHYSVEEWNAFLKKAPAAIFNYIALS
                     TGTGRNDAKKVSDIITANPKLNFICIDVANGYSEGFVNFVKLMRKNYPDKVIIAGNVV
                     TGEMVEELLLSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAIIECADAAHGLGGQIVS
                     DGGCSTPGDVAKAFGAGADFVMLGGMLGGHTESGGDLVERNGQKYKLFYGMSSETAMQ
                     KYVGGVAEYRASEGKTVEVPFKGDVENTIQDILGGIRSTCTYVGAPRLKELTKRTTFI
                     RVTEQENRVFTK"
     gene            complement(5027..5599)
                     /locus_tag="PK218_08280"
     CDS             complement(5027..5599)
                     /locus_tag="PK218_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPW98543.1"
                     /translation="MSANFFVEEKFNGITYDDRQLEYKEFEECIFTDCDFRTCSFQSV
                     AFIDCTFFNCNFQDARINYVSLRGVWFNGCNFTSVNFAMTDQVIYEFHFKDCLLDYAK
                     FYALKLKKMQFINCSMLSVDFMSSDLTEALFDNCNLRRAVFSETNLTKADLSTSYDYA
                     LDLDKNKVKKAVFSTDGLKGLLEKYQLVIK"
     gene            5642..6166
                     /locus_tag="PK218_08285"
     CDS             5642..6166
                     /locus_tag="PK218_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPW98544.1"
                     /translation="MDFQTSFFTYCPNCQSTDFSFKNVTKFQCNACAFEYYHNIAAAV
                     AVVFTFEDKILFTVRNENPDIGMLDLPGGFIDPKETAEEAACREIKEELDLDILPTSL
                     NYITTSPNEYLYKDVPYRTMDLFYECPLTNDKLSIKAKTEIKDLVWIKRNEIDLTKIG
                     FVSIRKVIGEFYLG"
     gene            complement(6500..6892)
                     /locus_tag="PK218_08290"
     CDS             complement(6500..6892)
                     /locus_tag="PK218_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPW98545.1"
                     /translation="MNLESPKVTVEKSAQHLFDALSDVKNFEKLMPENISKFEVLGDD
                     IFNFGLKGMPEIKLRMKETTPTSKVVLAAASDKLPFTLTANIDTLSESSSAVQLHFNG
                     EFNPMMAMMIKGPISKFIETLAQNMHKL"
     gene            complement(6911..7552)
                     /gene="pyrE"
                     /locus_tag="PK218_08295"
     CDS             complement(6911..7552)
                     /gene="pyrE"
                     /locus_tag="PK218_08295"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPW98546.1"
                     /translation="MIFNKDTAEKTAELLLQINAIKLNPKNPFTWASGWKSPIYCDNR
                     LILSFPPIRNFVREEFSKHIEKEFGKPDVIAGVATGAIGIGMLVAEYMGLPFVYVRPE
                     PKKHGRQNQIEGFLQSGQNVVVVEDLISTGNSSLMAVEALKEAGAHVKGMVAIFTYGF
                     DVAKDNFKKANVDLNTLADYSHLLDLAVAKKYITEEEQITLQEWQVSPSTWNG"
     gene            7560..8159
                     /locus_tag="PK218_08300"
     CDS             7560..8159
                     /locus_tag="PK218_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPW98547.1"
                     /translation="MYKVFVNDKPLFLTNEVHKETDFQLFLLESVDIEQLIVKMYQNK
                     IQKAYLYFPDEKAILKKLKEKIPVCKAGGGLVYNKKGEVLFIKRNGKWDLPKGGKDKG
                     EMMEETAMREVEEETGVNGLEIVKKLQKTYHVFQRNGKYKLKVTHWFEMKTDFDGKTN
                     PQKEEGIKKAVWLNPKEINEAMTNSYENIKLLFEEENQL"
     gene            8229..9575
                     /locus_tag="PK218_08305"
     CDS             8229..9575
                     /locus_tag="PK218_08305"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW98548.1"
                     /translation="MTNYHHSNNLLLNLGIESLNEMQLAAQETILRDNNVLLLSPTGS
                     GKTLAFLLPIFELLEGEGAEVQCLILVPSRELGLQIEQVWKKMGTNYKVNVCYGGHSI
                     DTEIKNLSNPPAVLIGTPGRIADHIDRGTFKVDNLQMLILDEFDKSLQLGFHEQMSFI
                     ISRLPKLNKRILVSATSGIEIPRYTQVIHPTVLDFIPTEEEKINLSMKMVVSNEKDKI
                     NSLFNLICSLKSQSALIFCNHRDAAERISDLLNEKGIYTTYYHGGMDQDERERALIQF
                     RNGSVSYLVTTDLAARGLDIPEMKHVIHYHLPSKEDEFTHRNGRTARMLASGTAYIIT
                     HESEKKLDYIDYSMPVLNVENAITLPKPPEFKTIYISGGKKNKLNKIDIVGFFSQKGK
                     LEKGDLGLIEVKDFVSFAAVKAKKVKDLLNAIRDEKMKGKKFKIEVARKVIKKEEE"
     gene            complement(9588..10055)
                     /gene="coaD"
                     /locus_tag="PK218_08310"
     CDS             complement(9588..10055)
                     /gene="coaD"
                     /locus_tag="PK218_08310"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPW98549.1"
                     /translation="MNKKAVFPGSFDPITLGHYDIIKRGISLFDEVIVAIGINADKKY
                     MFSLEERKYFIEEAFKNEPKVKVITYEGLTIDLCRKLDAEFILRGLRNPADFEFEKAI
                     AHTNRKLSKIETVFLLTASKTSYISSSIVRDVIRNNGDYTVLVPDSVQVKKHK"
     gene            complement(10048..10668)
                     /locus_tag="PK218_08315"
     CDS             complement(10048..10668)
                     /locus_tag="PK218_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98550.1"
                     /translation="MFINFGTKATNLQNGQIINVDCPSCEKVTTMKYSVFGKYGYAFW
                     IPFFPIKKITVAECNSSYKTFDYPDLYESIRTKIQRETEKNPVRYPVWMLSGVFILFA
                     LMGYGIYDSKMTDLNNEVYIKNPKAGDVYYLKASNGHFTTLKIDKVSRTEVYITNNDF
                     EIDLESDIFTIDESKNYNKFKDTINVLNLQESLNNETIIKIIRNHE"
     gene            complement(10671..>10884)
                     /locus_tag="PK218_08320"
     CDS             complement(10671..>10884)
                     /locus_tag="PK218_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPW98551.1"
                     /translation="RNVAKKAYEILKMTGFSRSEFILVNDEPFMLEMNTIPGLTNESL
                     IPQQANEAGISLEELFSNAIELALKK"
BASE COUNT         3658 a         1827 c         1864 g         3535 t
ORIGIN      
        1 attaacattc gaatgttttt atttcttgta aaaaggcaat tttacgactt tagctttaat
       61 gtctttattt ctaattttaa tataaatttc actatcgaca ccagcaaatt ctgtttttac
      121 gtagcccatt ccaatggcaa ttcctaaaga tggcgattgt gttccagaag ttacgatacc
      181 aatattgtta ccatcagcat caactatttc ataatcatga cgaggaattc ctctgtcgat
      241 taattcaaaa ccaactaact ttcttgttac accagcttct ttttgcttct ttaggttttc
      301 agaattcaca aattctttag taaatttagt aatccaaccc aatccagctt ctagtggaga
      361 agtagtatca ttaatgtcat ttccatacaa acagaaaccc atttctaaac gcaaagtatc
      421 acgagctgct aatcctattg gtttaattcc ataagaagca cctgcttcaa aaactttgtt
      481 ccaaacttgt tcaacttctg aattcttgca atatatttca aatccgcctg aaccagtata
      541 tcctgttgca gaaatgatta catgttctat accagcaaaa tcggctactt caaaatgata
      601 gtattttata gctgataagt caacagaagt taaactttgc atagcttcaa ctgcttttgg
      661 accttggatt gctagtaaag aataatcttc tgacaaatca cgcatttcaa catctaaatc
      721 gttgtgcgaa gaaatccaat tccaatcttt ttcaatgttt gaggcattaa ctaccaataa
      781 atattgatgc tcttttattc tatatataat taaatcatca acaatgccac catcattatt
      841 tggtaaacat gaatattggg cacgaccaat ttcaagaaca gaagcatcgt tagacgtaac
      901 tttttgaatt aatgccaaag cattttcacc cgataaaaga aattcgccca tgtgtgatac
      961 gtcaaaaacg cctacaccat tacgtacggt ttcatgttca atattcacac cttcatattg
     1021 aacaggcata ttatagcctg caaatggaac taattttgca cctaattgtt cgtgaacatt
     1081 tgttaaagca gtatttttca ttttattgta atatagtttt tgtatttgat aaggcttttt
     1141 taattaaata tttactttct gttataaggt ggtaataatt ttgttgaaac ctcaccaaaa
     1201 ccaattctaa ctgaattgtt tttacaaaat cctttcataa taactgtatc gttatcttga
     1261 ataaatttgc gttctgttcc atcgtttaaa gtgattgggt tttttccacc ccaagttaat
     1321 tctaacattg atccataact atcaggagta ggaccagaaa ttgtacctga acccatcatg
     1381 tcgccagaat ttattctaca tccattagat gtatgatgtg ctaattgttg tgccattgac
     1441 caatacatat atttaaaatt ggattttgat agtagtgttg gctcattcga tttttcagta
     1501 tcaatataaa cttctaaatt aatgtcaaaa gcattttttc ctttttgttg taaataaggt
     1561 agtggtttcg gctcttgatt tggaccttta actcgaaacg gttccaaagc atccatggtt
     1621 actatccaag gagaaattga agaagcaaag ttttttgcta aaaatggtcc aagaggcaca
     1681 tattcccatt tttgaatgtc tctagcgctc caatcgttta ataaaaccat tccaaaaata
     1741 taatcttctg cttcgtttac aggaatgttt tcgcccatta cattggcatc agtagtaata
     1801 aaagcagttt ctaattcaaa atcaaccaat ctcgaagaac caaaaacggg ttcggtttca
     1861 ccagcaggaa gtgtttgtcc cattggtctg tgtacaggaa ttcccgaagg tataatagta
     1921 gaacttcttc catgataacc tacaggaata tgtaaccagt ttggtaataa agcattttct
     1981 ggatcacgga acatttttcc aacattcgtt gcatgttctt tactcgaata gaaatcagta
     2041 taatcaccaa ttaaaactgg taattgcatt tctacttcat ccatattgaa aatgacaata
     2101 tctctgtgtt taggattatc tctaagtttt ggattgttta tgtcaaaaat atcgccaata
     2161 cgatttctaa ccatacgcca tgtttttttc ccgtcagaaa taaagtcgtt gagcgtatct
     2221 tgcataaaca tatcatcggt caactcaatg ccatcaaaat aatgtaattg ttgaagcgca
     2281 cctaaatcga ctgcataatt acctatgcgt gttccaatgg taatcacatc atctttggtt
     2341 aaaaaaacac caaaaggaat attttgaatt ggaaaatcac tgttgtctgg aacgtctaac
     2401 catgatttac ggcttgggtc attggtattt atgggcattt tttatttttt tagttgttga
     2461 aaaatttagt aatcaaatat atcatttcct attaaagaaa caaacgattt tttgtaattt
     2521 tgaactcaat taatcgaaat actgagaaat gcaacgcgac gaacaaattt ttgaccttat
     2581 tcttgatgaa caagaaagac aaattcacgg attagaacta attgcttcag agaactttgt
     2641 tagcgaccaa gtaatggaag cagcaggttc tgtgttaacc aacaaatatg ccgaaggcta
     2701 tcctggcaaa cgttattatg gtggttgtga agtagttgat gttatagaac aaattgctat
     2761 tgatagagcc aaaacgcttt ttggtgctga atatgttaac gtgcaaccac attcgggttc
     2821 acaggcaaat actgctgtat atgcagcatg tttaaaacct ggtgataaaa tattaggttt
     2881 tgatttatcg cacggtggtc atttgacaca cggctctcct gtaaattttt ctggaaaatt
     2941 atacaatcct gttttttatg gtgttgacca agagacgggt gttttaaatt atgacaaaat
     3001 tcaagaaata gcaaccaaag aacaaccaaa aatgattatt gctggtgctt ctgcttattc
     3061 tcgggatatg gattttgctc gttttagagt catagccgat agtgttggag cattgttatt
     3121 ggcggatatt tcacatcctg cgggattgat tgcaaaaggt ttaatgaatg acccaattcc
     3181 tcattgtcat atagttacaa caactacaca caaaacgctt cgtggtccta gaggcggaat
     3241 gattatgatg ggtaaagatt ttccaaatcc atggggattg accacaccaa aaggtgaaat
     3301 aagaatgatg tcgcatgtgt tagacatgtc tgtttttcct ggaaatcaag gtgggccatt
     3361 agagcatatt attgcggcta aagcggttgc ttttggagag gctttgtctg atgaattttt
     3421 tacgtatcaa atgcaagtac agaaaaatgc aaaagcaatg gcagaagctt ttgtaaaaag
     3481 aggttataat attatttcgg gaggaacaga caatcacatg atgttgattg atttgcgaaa
     3541 caaaaatatt tctggaaaag aagcggagaa tgctttggtc aaagctgaaa ttacggtgaa
     3601 taaaaatatg gtaccatttg atgataaatc accatttgta acttcaggta ttcgtgtggg
     3661 tacaccagcc gttacaactc gtggtttgat ggaagctgat atggaaacaa tcgttgattt
     3721 tatcgataga gtgattatga atcacaccaa tgaagaactg cttgaaagta tagcggatga
     3781 agtaaacgaa atgatgagtg aaagagccat ctttgtttat taattaatag ttagctacaa
     3841 tattcaaaac ctccaaatac ttttgtttgg aggttttttt atgtgcttta tttttttgtt
     3901 aataaatcac taattacttt ttactaaaaa caacgtgttt tttacatcta tattctacat
     3961 ttgatagtat caaaatgtag tagctatgcg catagaaacc gaccttaaat tgggatttaa
     4021 agatgtaatg attagaccaa aacgttcaac actaaagagt agagcagagg tgtctttgga
     4081 acgaacgttt aaattcatgc acagcactta tgaatggaaa ggcgttccca ttatggcagc
     4141 caatatggat actgttggta cttttgaaat ggcgttggcg ttggctcaaa aggatatttt
     4201 tactgccatt cacaaacatt attctgttga ggaatggaat gcttttttaa aaaaagcacc
     4261 tgcagcaatc tttaactaca ttgccttaag cacaggaaca ggaagaaacg atgccaagaa
     4321 agtatcggat ataatcaccg caaatcctaa attgaatttt atttgcattg atgttgccaa
     4381 tggctattca gaagggtttg taaattttgt gaaactgatg cgcaaaaatt atcccgataa
     4441 agtaattatt gctggaaatg tagttacggg cgaaatggtg gaagagttac ttctttcggg
     4501 tgctgatatt gttaaagtag gcattggtcc aggttctgtt tgtactactc gtgtgaaaac
     4561 aggtgttggt tatccgcaat tgtctgccat tattgaatgt gccgatgcag ctcatggttt
     4621 gggcgggcaa attgttagcg atggtggttg ctcaacgcca ggtgatgttg caaaagcttt
     4681 tggtgctggt gccgattttg ttatgcttgg cggaatgtta ggcggacata ccgaaagcgg
     4741 tggcgatttg gtggaacgca atgggcagaa atacaaactc ttttatggta tgagttctga
     4801 aacagcaatg caaaaatatg ttggtggcgt tgcagagtat cgtgctagcg aaggaaaaac
     4861 tgtagaagtt ccttttaaag gcgatgttga aaacaccatt caggatattc ttggaggcat
     4921 tagaagtaca tgtacttatg ttggagcacc acgattaaaa gaactaacta aacgcactac
     4981 tttcattaga gtaaccgaac aggaaaatag agtatttacc aaataactat ttaatcacta
     5041 actgatattt ctccaaaagt ccttttaaac catcagtaga aaaaacggct ttttttactt
     5101 tgtttttatc caagtccaag gcataatcat aactagtaga aaggtcggct ttggttagat
     5161 tagtttcact aaaaacggct cttctcaaat tgcaattatc aaacaacgcc tctgttaaat
     5221 cagaagacat aaaatctaca gatagcatgc tgcaattgat gaattgcatt tttttaagtt
     5281 tcaaagcata aaacttggca taatctaaca aacaatcttt aaagtgaaat tcataaatca
     5341 cttgatcggt catggcaaag ttaacactgg taaagttaca cccattaaac caaacaccac
     5401 gcaaggaaac atagttaatg cgcgcatctt gaaaattaca attaaaaaaa gtacaatcaa
     5461 taaaagcaac cgattgaaag ctacaggttc taaaatcaca atcggtaaaa atgcattctt
     5521 caaattcttt gtattctaat tgtctgtcgt cgtaggttat accattaaat ttttcttcaa
     5581 caaagaaatt tgcgctcata attgtaaatt tgtgcaaata taaccaacca aaagtaaccc
     5641 tatggatttc caaacttctt tttttaccta ttgccccaat tgtcaatcaa cagattttag
     5701 ttttaaaaat gttaccaagt ttcagtgcaa cgcttgtgcc ttcgaatatt atcataacat
     5761 tgcggcagcc gtggcagttg tttttacttt tgaagataaa atattgttta ccgttcgcaa
     5821 tgaaaacccc gacattggta tgctcgattt gcctggtgga tttattgatc caaaagaaac
     5881 tgccgaagaa gccgcatgcc gagagattaa agaagaatta gatttggata ttttgccaac
     5941 cagtttaaac tacatcacta cttcgcccaa cgaatatttg tataaagatg tgccctatag
     6001 aacgatggat ttgttctacg aatgtccttt aacaaatgat aaactctcta taaaagccaa
     6061 aacagaaata aaagatttgg tttggataaa acgcaatgaa attgatttga ctaagattgg
     6121 ttttgtgtct attcgtaaag tgattggaga gttttatttg ggatagatga gtttttaacg
     6181 gttttattct ctaatcatgc aaaaccaatt tggattccta attaccttag tggtaaaaaa
     6241 aatgcaccaa taaaatgagt ctttgtatca gttacccgac atctcaaatc taatttttat
     6301 tataaagtaa tttaaaatcc tattaaaata ataagctaaa ccataaacta aagataataa
     6361 atttataaca cccaaatcac tttatattaa aaaaaatcct agaataatta cttcattaaa
     6421 acatagatgg ccaccagaat atggcttttt tgaacatgtt caaaagagag tattttagga
     6481 gttaaagatt cgggtagtcc tataacttgt gcatgttttg cgccaaagtt tcaataaact
     6541 tcgagatagg acctttaatc atcatcgcca tcattggatt gaattctcca ttaaaatgaa
     6601 gttgtactgc actagagctt tccgacaaag tatcgatatt agctgtaagc gtaaaaggta
     6661 gtttatcact cgccgctgca agaactactt tgctagtagg tgtggtttct ttcatgcgca
     6721 atttaatttc gggcattcct tttagtccaa aattaaatat atcatcgccc aatacttcaa
     6781 actttgaaat attttcgggc atcaacttct caaaattctt tacatccgac aatgcatcaa
     6841 acaaatgctg tgccgatttt tctacagtaa cttttggact ttctaaattc atttttttct
     6901 attttctatc ttaaccattc cacgttgaag ggctaacctg ccattcttgt agtgtaattt
     6961 gttcttcttc ggtaatgtat ttttttgcca ctgccaaatc caaaagatga ctataatctg
     7021 ccaaagtgtt taaatcaaca ttggctttct taaagttatc tttggcaaca tcaaaaccat
     7081 aggtaaagat tgctaccata cctttaacat gtgcgccagc ttcttttagt gcttctacag
     7141 ccatcaaact gctgtttcca gtactaatta aatcttcgac taccactacg ttttgaccgc
     7201 tttgcaaaaa gccttcaatt tggttttgtc ttccgtgttt ctttggctcc ggacgaacat
     7261 atacaaatgg cagtcccatg tattcagcaa caagcatgcc aatgccgatt gctccagtag
     7321 ctacaccggc aataacatcg ggttttccaa attctttttc aatgtgctta gaaaattctt
     7381 cacgaacaaa atttctaata ggtggaaatg agagtattaa acgattatcg caataaattg
     7441 gcgatttcca acccgaagcc catgtaaaag gatttttagg attcaattta attgcattta
     7501 tttgtaaaag caattcggct gttttttcgg ctgtgtcttt attaaaaatc atagtacaaa
     7561 tgtataaagt ttttgtcaac gataaaccac tttttttaac aaatgaagtt cataaagaaa
     7621 ctgattttca gttgttcttg ttagaaagtg ttgatattga gcaactcatc gttaagatgt
     7681 atcaaaataa aattcaaaaa gcctatttat attttcctga tgaaaaagcc attctaaaga
     7741 aactaaaaga aaaaataccc gtttgcaaag caggtggagg attagtttat aacaaaaaag
     7801 gcgaagtgct cttcattaaa cgcaatggaa aatgggattt gccgaaaggt ggaaaagata
     7861 aaggcgaaat gatggaagaa actgctatgc gtgaagtgga agaagaaacg ggtgtaaacg
     7921 gattggaaat agttaagaaa cttcaaaaaa cgtatcatgt ttttcaacgc aatggcaagt
     7981 ataaactaaa agtaacccat tggtttgaga tgaaaactga ttttgatgga aaaactaatc
     8041 ctcaaaaaga agaaggtatt aaaaaagcag tttggttaaa tccaaaagaa ataaatgaag
     8101 ccatgaccaa ctcctatgaa aatattaaac tgttatttga agaggagaat caattataat
     8161 aaagttacat ttttcgttta atttgcaatt cgtaattttt aattcataat tggctttatc
     8221 tttgccaaat gacaaattac caccattcca acaacctact tttaaattta ggcatcgaaa
     8281 gcttaaatga aatgcaactt gctgcacaag aaacaattct tagagataat aatgttttgt
     8341 tactttctcc aacgggttca ggaaaaacgc tagcattctt actaccaatt tttgaattgt
     8401 tagaaggaga aggtgcagaa gttcaatgtt taattttagt gccatcaaga gaactaggct
     8461 tgcagataga acaagtttgg aagaaaatgg gaacaaatta taaagttaat gtatgttacg
     8521 gcggacattc catcgatacc gaaattaaga atctatccaa tccaccagca gttttaatcg
     8581 gaacaccagg aagaattgct gaccatattg atagaggaac ttttaaagta gataatcttc
     8641 aaatgttgat tttggatgag tttgataaat cgttgcagtt gggttttcat gagcaaatgt
     8701 cttttatcat cagtagatta ccaaaattaa acaaacgtat cttagtatcg gcaacatctg
     8761 gtattgaaat tccaagatat acacaagtaa tacatcctac ggttttagat tttattccga
     8821 ctgaagaaga gaaaataaac ctttcgatga aaatggtggt ttctaatgaa aaagataaga
     8881 ttaacagctt gtttaacttg atttgttcgt tgaaatcgca atcagctttg atattctgca
     8941 atcaccgaga tgccgcagaa cgcattagtg accttttgaa cgaaaaagga atctacacca
     9001 cttattatca cggcggaatg gatcaagatg aaagagaacg tgctctaatt caattcagaa
     9061 acggaagtgt ttcttattta gtgaccaccg atttggcagc acgtggtttg gacattcctg
     9121 aaatgaagca tgtaattcat tatcatttgc catcaaaaga agacgaattc acccatagaa
     9181 atggtagaac agccagaatg ttagcttctg gaacggcgta tatcattaca cacgaaagcg
     9241 aaaagaaact ggactatata gattattcga tgccagtttt aaatgttgag aatgctatca
     9301 cgttgccaaa accacccgaa tttaaaacaa tttatatcag cggtggtaag aaaaacaaac
     9361 taaacaaaat agacatcgta ggcttctttt cccaaaaagg aaaactcgaa aaaggcgatt
     9421 taggattgat tgaagtaaaa gactttgtat cttttgcagc cgtaaaagcc aaaaaagtaa
     9481 aagacttact caatgctatt cgagacgaaa agatgaaagg aaagaaattc aaaattgaag
     9541 tggctcgaaa agtaataaag aaagaagaag agtagtcttt tttaagttta cttatgcttt
     9601 ttaacttgaa cagaatcggg aactaaaacc gtataatcac cgttatttct aatgacatcg
     9661 cgaacaatgc tcgaactgat ataggatgtt ttagaagccg ttaaaagaaa aacagtttct
     9721 attttagaca gctttctgtt agtatgagca atagcttttt caaattcaaa atcagcagga
     9781 ttacgcaaac cacgaagaat aaattcggca tctagttttc tgcacaaatc aatggtcaaa
     9841 ccttcgtagg taatgacttt aacttttggt tcgtttttaa acgcttcttc aataaaatac
     9901 ttgcgttctt caagagagaa catgtatttt ttatcagcat taatacctat agcaacaatc
     9961 acttcgtcaa aaagcgaaat gcctctttta ataatatcat aatgtccaag agtaattggg
    10021 tcaaacgatc cagggaaaac agctttttta ttcatggttt ctgataattt tgattatagt
    10081 ttcattattt aaagattctt gtaaatttaa tacatttata gtgtctttaa atttattata
    10141 attttttgat tcatcaatcg taaaaatatc agattctaaa tcaatttcaa aatcattgtt
    10201 ggttatgtaa acttcagttc tagatacttt atcaattttt aatgtggtga aatgaccatt
    10261 tgatgctttt aaataataaa catctccagc ttttggattt ttaatatata cttcattgtt
    10321 taagtcggtc atttttgaat cataaatgcc atatcccatt aatgcaaaaa gtataaatac
    10381 accagataac atccaaactg gatatcgtac tggattcttt tcggtttctc tttgaatttt
    10441 cgttctaata ctttcatata aatcaggata gtcaaaagtt ttataagaag aattacattc
    10501 ggctacagta atttttttta ttggaaaaaa aggtatccaa aatgcatacc catattttcc
    10561 aaaaacactg tatttcatag tggttacttt ttcgcagcta ggacaatcaa cattaataat
    10621 ttgaccgttt tgtaaattag ttgctttggt accgaaatta ataaacattt ttactttttt
    10681 aaagccaact caatagcatt actaaacaat tcttccaaag aaattccagc ttcattggct
    10741 tgttgtggaa ttaaactttc gttagttaaa cctggaatgg tattcatttc caacataaaa
    10801 ggttcgtcat tcaccaaaat aaactcacta cgagaaaaac cagtcatttt taaaatttcg
    10861 taagcttttt tggcaacatt tcta
//
LOCUS       DAOOBH010000078        10855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_7678_length_10855_cov_5.202778, whole genome shotgun sequence.
ACCESSION   DAOOBH010000078 DAOOBH010000000
VERSION     DAOOBH010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10855
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7678_length_10855_cov_5.202778"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PK218_08325"
     CDS             complement(<1..1079)
                     /locus_tag="PK218_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPW98552.1"
                     /translation="MKKIFLSLFLLIIYSISFGQVKDSIPQKKKIDSVNTVLLNQYNQ
                     KLIELKEEQSKDSLKKVALENELKGLKNTDNLKKEELLSELNRLKDSENNRLELKKAR
                     IDSLKATATGFPVKGFFNDSLFVIYSKIGSFSAKERADAISKRIAELKDNVVFDKKSL
                     SIDATESNIDIVFKDKTIISITENDAIWNGTTKQKLAEEYKKIITNSVLKYKSETSFG
                     TLLKEIGLALLVILILVLLIVYLKKFFRWTAKKIEEQEGKLIQGIKLKNYVLFDAKRE
                     ISVLLSINTLLKWVFIGICIYIVLPILFNIFPWTQDFSKTLFGYILNPLKKMTTSLWH
                     YLPNLITIIVIVVVFRYIIKGIHFL"
     gene            complement(1081..1851)
                     /locus_tag="PK218_08330"
     CDS             complement(1081..1851)
                     /locus_tag="PK218_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPW98553.1"
                     /translation="MKKILLFLLIISFSKSFSQYNETIRTGRPGQAIGAFSVGKNILQ
                     FQQGIDVYSFDNAEYKPFGIVSNNIIRFGIIEKIEISALIDYQYDQQKYDNETTFQSG
                     LSNFHLGFRIHLNNQKGWVPATAFQMRLKFPKISKDYGSNNFAPIGVFVANWNLPKNM
                     SIASNWILSYNGNDANPTGKYVLNFGFPIYQKLSGFIESYGQINNNIFQTRFDTGFAY
                     LVNNNFQLDISSGFGNNQNVTDYFISTGISWRVLNFRK"
     gene            complement(1905..4523)
                     /gene="cphA"
                     /locus_tag="PK218_08335"
     CDS             complement(1905..4523)
                     /gene="cphA"
                     /locus_tag="PK218_08335"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPW98554.1"
                     /translation="MKILKIQALRGPNIWSINRKRLIQMRLDLEEMEQFPTNKIEGFR
                     ERIEAMFPTMIEHRCSEGTRGGFFSRIERGTWMGHVIEHIALEIQTLAGMETGFGRTR
                     ETKTPGVYNVVFSYTEENVGLFAAESAVAIAEALISGAEYDVEGDIQKMREIRERVRL
                     GPSTGSIVEEAVSRDIPWIRLGTNSLVQLGYGINQMRFQATITCKTSNIAVDIACNKE
                     ETKRMLDMASIPVASGGICVDEEDLDDVIKKIGFPIVLKPLDGNHGKGASINVKNRED
                     AVEGLAYAQKYSRRVIVEKFITGFDFRVLIIDNKLVAAAKREPAHVKGDGVKTIQQLI
                     DETNLDPRRGYGHENVLTQIDVDRDTTDLLEKLEYTLETIPTKDEVVYLKSTANLSTG
                     GTSVDVTDMMHPENIFLCERISRVIGLDVCGVDIMAVNLTQPLKENGGCILEVNAAPG
                     FRMHLAPSEGLPRNVAAPVIDMLYPPGKESRIPIIAVTGTNGKTTTTRLIAHIVKNNG
                     FKVGFTTSDGIYIQNHMMEKGDTTGPISAEYILKDPTVEFAVLETARGGILRSGLGFG
                     RCDIALITNIQEDHLGLSDIHTLEDLARVKSVVVRSVKKDGWAILNAEDEQCMKIANE
                     LSCNIAYFSLDEDNPVAKKLSKEGKIVAVYENGYITIKKGEWKIRIEKATHVPLTLGG
                     KARFMIANVLAASLAGYLHGFKTEDISLSLQTFIPSAAQTPGRMNIFEFKKFKVLIDF
                     AHNPSGYRGVEDFLTSVEATKKIGIIAGVGDRRDEDIRECATIAARMFDHIIIRQEKH
                     LRGRTEEEIIGLIMEGIEASGRNVTTEIIKREVEAIKHAISNAEDGSFITALSDVVTN
                     AIEIVQEYLDKENEEV"
     gene            complement(4700..5542)
                     /locus_tag="PK218_08340"
     CDS             complement(4700..5542)
                     /locus_tag="PK218_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW98555.1"
                     /translation="MKRIQHFILVKSVGLYVNILSFVRPEKAKKLAYQLFSQPRKGRI
                     DSTKLPKTLVNTEKETFSFQNETFQTYMWRGNEEIILLIHGWESNSSRWKKLLPYLLP
                     LGKTIIAIDAPAHGLSSGKEFSAPKYAEYINVVTQKYQAKLLIGHSIGGGAIACYLNK
                     YNDLSVQKVILLGSPSDFKLLNNNYAALLSLNAKVKILLEKHIQEKFNIHLDDFKGHL
                     FAENFSQKALIAHDIDDKIVLVEEGKKYASTWKNATYIETKGLGHSLHGKDLYKTITD
                     FIKL"
     gene            complement(5576..6355)
                     /locus_tag="PK218_08345"
     CDS             complement(5576..6355)
                     /locus_tag="PK218_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98556.1"
                     /translation="MATTKELSVEDKLRALYDLQIIDSRIDEIRNVRGELPLEVEDLE
                     DEVAGLSTRLEKFKADLVSIEDQIKAKKNAIEAHQASIKRYTEQQKNVRNNREFNSLT
                     KEVEFQELEIQLAEKHIKEMKASIEHKKEVVANSKEKLDQKTTHLKHKKAELNDIMAE
                     TQKEENFLMQKSAEYEAKIEERLLAAYKRIRSSVINGLAVVSIERGASAGSFFTIPPQ
                     TQVEIASRKKILTDEHSGRILVDAALAEEEREKMEKLFAKM"
     gene            complement(6359..7474)
                     /locus_tag="PK218_08350"
     CDS             complement(6359..7474)
                     /locus_tag="PK218_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPW98557.1"
                     /translation="MKVKELFPILEELAPLAYAEEFDNVGLLVGNQEQELTGILVCHD
                     ALEAVIDEAIAKECNLVVCFHPILFSGLKKITGKNYVERAVLKAIKNDIAIYAVHTAL
                     DNHPNGVNKILSDALGLINTKVLVPKQNFIQKLVTYTIPENEVQLRNALFNAGAGKIG
                     NYENCSFNSQGIGTYMGNENSNPEIGERFEFIEAKEIKIEVTFEKQLQSKILKALFSN
                     HVYEEIAYEIYHMQNEHQNIGLGMIGELETAMNAKDFLHFVKEKMQCGNIRHSQLVEK
                     PIKKVAVLGGSGSFAIKNAILANADVFLTADLKYHNYYEAENQLVLADIGHFESERYT
                     KNYIVDFLKEKITNFAPAFSIVKIVLSEENTNPVKYL"
     gene            complement(7519..8082)
                     /locus_tag="PK218_08355"
     CDS             complement(7519..8082)
                     /locus_tag="PK218_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98558.1"
                     /translation="MSFKLKFTFLFFITIQLGFSQEIENAAILKVLLTKAYVNEKVIV
                     KNRLQLLNFYCNKAPNNEETLEVISKNDLLKKNAEIIKKQIDITINEDWSKEFNILFT
                     DQHQYLKSKVNDCLSLEDFQKVSTQYGENNQRLMIVSKPMYFSKNYCLVKVAFYRNIE
                     HNSGCFYLFEKKNTIWTIIETLNEWST"
     gene            8166..9185
                     /gene="lpxK"
                     /locus_tag="PK218_08360"
     CDS             8166..9185
                     /gene="lpxK"
                     /locus_tag="PK218_08360"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPW98559.1"
                     /translation="MIILRKLLFPFAVLYGIITSIRNFLYNQGILRSYSFSIPLIAVG
                     NLSVGGTGKTPQIEYLIRLLSKDYKVAVLSRGYKRKSEGFVLGNKNSTAEMLGDEPFQ
                     IFQKFPQVQVAVDVDRKKGIEQLLKLENKPEVILLDDAFQHRRVKAGFYVLLTAYDDL
                     YCDDFMLPTGNLRESRNGVKRSDLVVVTKCPTALSKEEMKRIIKKLGTTKKVFFSTIA
                     YDDFVYNENNSTSIYEIKSKEKLLLAGIAKPALFFDYLKQPNDDVMAFPDHHHFTEND
                     IKNINNKAKNKKIITTEKDYVRLNNHTLQSDIYYLPIKSKFLSDTDDFNKTIKNYVGT
                     STRNR"
     gene            9157..9969
                     /locus_tag="PK218_08365"
     CDS             9157..9969
                     /locus_tag="PK218_08365"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPW98560.1"
                     /translation="MWEQVQETVNYIKSKTGYTPDYGVILGSGLGSFTNDIAIEFTMP
                     YNEIPNFPVSTVEGHKGALVFGKIGDKKVVAMQGRFHYYEGYSMQEVTFPVRVMKYLG
                     INKLIVSNASGGVNPNYHVGVVAIITDHINMFPEHPLRGKNDTRFGPRFVNMSEPYDR
                     KMIVKAKELAKEHNLEVFEGVYLGLQGPTFETLAEYKMVINMGADCVGMSTVPEVIVA
                     KHMDMDCFGVSVITDMGDVDSIDSISHEEVLEAAKSAEPTVRTLIKQLILNY"
     gene            complement(9944..>10855)
                     /locus_tag="PK218_08370"
     CDS             complement(9944..>10855)
                     /locus_tag="PK218_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW98561.1"
                     /translation="KLKDLLNNIQNSLKELAKVNNNKFILDIDENVPNVVIGDPTKMS
                     QIFLNLINNALKFTKNGEVKVIVELFGIKENSATIHFSVMDTGIGIAKDKQETIFDSF
                     AQGSIEINRKFGGTGLGLAIVKKLIDLLGGEIKLESELGKGSTFYFDLAFEISENQVD
                     REKEEILLLDTDIDTMLEGKNVLIVEDNKINQMITKKMVEKKGMITKIIDNGEDAIEE
                     IRNNNVYDLILMDVHLPSINGTIATREIRKFNKSLPVIALTAISLNENRDILLGFGMD
                     DVITKPFDINTFYTVISKVLSNSKSVA"
BASE COUNT         3425 a         1789 c         1600 g         4041 t
ORIGIN      
        1 ttaagaaaat gaattccctt tatgatgtat ctaaaaacta ctacaattac aataatggta
       61 attagatttg gcaaataatg ccaaagactt gtagtcattt tttttaatgg atttaagata
      121 tagccaaata atgttttgga aaaatcttgt gtccatggaa aaatattaaa aagaataggt
      181 aaaactatat aaatacatat accaatgaaa acccatttta atagggtgtt gattgataac
      241 aatacgctaa tttctctttt agcatcaaat aaaacataat tttttaattt aattccttga
      301 attaattttc cttcttgttc ttcaattttt tttgctgtcc aacggaagaa tttttttaaa
      361 taaacaatta gtaatacaag tattaaaatt acaagtaaag ccaaaccaat ttcctttaat
      421 aaggttccaa aacttgtttc agatttgtat tttaaaacag aattagttat gatttttttg
      481 tattcttctg ctagtttttg tttggtagtc ccattccaaa tggcatcatt ttcggtaata
      541 ctaatgatgg ttttatcttt gaaaacaata tcgatattgg attctgtagc gtctatagaa
      601 agtgattttt tgtcaaaaac aacattgtct tttaattcag caattctttt tgaaatagca
      661 tctgcacgtt cttttgcaga gaaactacca attttactat aaataacaaa taaagaatca
      721 ttgaaaaaac cttttacagg aaatccagtt gcggtagctt ttaatgaatc tattcgtgct
      781 ttttttaatt caagtctgtt gttttcagaa tcttttaatc gattgagttc agataaaagt
      841 tcttcttttt ttaaattatc ggtgtttttt aaacctttta attcgttctc aagagctact
      901 ttttttaatg aatctttgct ttgttcttct tttaattcga tgagcttttg attgtattga
      961 ttcaataaaa cggtgttcac tgaatcaatt tttttctttt gaggaatact atcttttact
     1021 tgtccaaatg aaatcgaata aatgataagt aagaataagg ataaaaatat ttttttcata
     1081 ttattttcta aaatttaaaa ctctccatga aattccggta ctgataaaat aatctgttac
     1141 attttgatta tttccaaaac cgcttgaaat gtccaactga aaattattgt tgactaggta
     1201 tgcaaaacct gtgtcaaatc gagtttgaaa aatattatta ttaatttgcc catagctttc
     1261 aataaaacca cttaactttt gatagattgg aaaaccaaaa ttcagtacat attttccagt
     1321 tggatttgca tcatttccat tgtaagaaag aatccaattg gaagcaatac tcatgttttt
     1381 aggtaagttc caatttgcca caaaaactcc aataggagca aaattattgc ttccataatc
     1441 tttggagatt tttggaaatt taagtcgcat ttgaaaagct gttgctggaa cccacccctt
     1501 ttgattattc aaatgaattc taaaacccaa atgaaaatta ctcaaacctg attgaaaggt
     1561 tgtttcatta tcatattttt gttggtcgta ttggtaatca attaaggcac taatttcaat
     1621 tttttctatt atcccgaagc gaattatatt atttgaaaca attccaaacg gtttgtactc
     1681 tgcattatca aatgaatata catctattcc ttgttgaaat tgtaatatgt ttttacctac
     1741 ggaaaatgca ccaatagctt gtcctggtct tccagttcgg atggtctcat tatattgact
     1801 aaatgatttt gaaaaagaaa taattaaaag gaaaagtaaa atttttttca tcctttataa
     1861 tataagttat ttttagcaaa aaagcaataa aaaatcatac ttttttaaac ttcctcattt
     1921 tctttatcca aatattcttg tacaatttct atagcatttg tgactacatc gctcaaagca
     1981 gttatgaaac tgccgtcttc agcattgctg atagcatgtt tgatcgcttc tacttcgcgt
     2041 ttgatgattt cagtggtcac atttcttcca gaagcttcta ttccttccat gattaaacca
     2101 atgatttctt cttcggttct tccacgtaaa tgtttttctt gacggatgat aatatgatca
     2161 aacattctgg cggcaattgt agcacattct ctaatgtctt cgtcacgtct atcgccaaca
     2221 ccagcaataa tgccaatctt tttagtagct tctaccgacg ttaaaaaatc ttcaacacca
     2281 cgataaccag aaggattgtg cgcaaaatca attaatactt taaatttttt aaattcaaaa
     2341 atattcattc tacctggtgt ttgtgcagca ctaggaataa aggtttgtaa tgataagcta
     2401 atgtcttctg ttttaaatcc atgcaaataa ccagctaatg aagcggctag aacattggca
     2461 atcataaatc gagcttttcc accaagggtt aacggtacat gagtcgcttt ttcaattctg
     2521 attttccatt ctcctttttt gatggttatg tatccatttt cataaacagc tacaattttg
     2581 ccttctttgg ataatttttt agccactgga ttgtcttcgt ctaaactgaa ataagcaatg
     2641 ttacagctca attcatttgc aattttcatg cattgttcat cttctgcatt caaaattgcc
     2701 caaccatctt ttttgacact gcgaactact acacttttta ctcgtgctaa atcttctaaa
     2761 gtatgaatat cggataatcc taagtgatcc tcttgtatat tggtaatcag tgctatatcg
     2821 caacggccaa atcctaatcc agaacgtaag attccaccgc gagccgtttc taaaacagca
     2881 aattcaaccg ttggatcttt taaaatatat tcggcagaaa ttggtccagt agtatcacct
     2941 ttttccatca tgtgattttg aatgtatatt ccatcagatg tggtgaaacc aactttaaaa
     3001 ccattatttt taacaatatg cgcaatcaat cgggtagtag ttgtttttcc attggttcca
     3061 gtaacagcaa taatcggaat tctactttct tttcctggtg gataaagcat gtcaattact
     3121 ggtgctgcaa catttcttgg caatccttcg cttggcgcta agtgcattct aaatccaggc
     3181 gcggcattaa cttcaagaat acaaccacca ttttctttca atggttgcgt taagtttact
     3241 gccataatat caacaccaca aacatctaat ccaatcacac gtgaaatacg ttcgcaaagg
     3301 aaaatattct caggatgcat catatcagta acatctacgg aagttccacc agtactcaag
     3361 ttggcagtag attttaaata caccacttca tcttttgttg gaatggtttc taaggtatat
     3421 tctaattttt ctagtaaatc ggttgtatct ctatcaacat ctatttgggt taaaacattt
     3481 tcgtgtccat aaccacgtct tgggtcaaga ttggtttcgt caattaattg ttgaatggtt
     3541 ttaacaccgt cgccttttac gtgtgctggt tctcttttgg cagctgcaac taatttattg
     3601 tcaatgatta atactcggaa atcaaaacct gtaataaatt tttcaacaat aactcttcgg
     3661 ctgtattttt gagcataagc caaaccttct acagcatctt ctctattttt cacattaatg
     3721 gaagctcctt ttccgtgatt tccatctaaa ggctttaaaa caataggaaa cccaattttc
     3781 tttattacgt catctaaatc ttcttcatca acacaaattc caccgcttgc aacaggaata
     3841 gaagccatat cgagcatgcg ttttgtttct tctttattac aagctatgtc aacagcaata
     3901 ttggaagttt tacaagtaat agtggcttga aaacgcattt gatttattcc atatccaagc
     3961 tgtactaatg agtttgttcc aagtctaatc caaggaatgt cccttgaaac ggcttcttca
     4021 acgatacttc ctgttgatgg accaagtcga acacgttctc taatttcacg cattttttgg
     4081 atatcacctt caacatcata ttctgctcca gaaattagag cttctgcaat ggcaactgca
     4141 ctttcggcgg caaaaagacc aacgttttct tcggtgtaac taaaaacgac attgtaaaca
     4201 ccgggtgttt tggtttctct tgttcttcca aaaccagttt ccattccagc taatgtttga
     4261 atttctaaag caatatgttc aatgacatgt cccatccaag tgccacgttc tattcttgag
     4321 aaaaatccgc cacgtgttcc ttctgaacaa cgatgttcaa tcatagtggg aaacatagct
     4381 tcaattcgtt ctcgaaaacc ttctatttta ttggttggaa attgctccat ttcctctaag
     4441 tctaatcgca tttgaattag tctttttcta ttgatgctcc aaatatttgg tccacgtaat
     4501 gcttgtattt ttaggatttt catatctatt ttggttgtag ttggtaatat gttaatgatt
     4561 ttgttaaaaa taacaaatta ttgtttcaat gcttcttttt ttacaaagaa atattgaaaa
     4621 ttatgttttg aaataatttt tgaataaaaa gctgttgaaa atcaaaagtt tgtaaaaatt
     4681 gaaactacag ttattgtctt tacaatttaa taaaatcagt aatcgtttta tataaatctt
     4741 tgccatgaag actgtggccc aaacctttgg tttcaatata tgtagcgttt ttccatgtgg
     4801 aggcatattt ttttccttcc tcaactaaaa caattttatc atcaatatcg tgagcaataa
     4861 gtgctttttg tgaaaaattt tctgcgaaaa gatgtccttt gaaatcgtca agatgtatgt
     4921 taaatttttc ttgaatatgt ttttctaaaa gtatttttac tttagcattt aaacttaata
     4981 aagctgcata attgttgttt aataatttaa agtcagaagg tgaacccaat aaaattactt
     5041 tttgaaccga taaatcattg tacttattaa gataacaagc aatagcacca ccaccgatgg
     5101 aatgaccaat aagtaattta gcttgatatt tttgtgttac tacattgatg tattctgcat
     5161 attttggcgc gctaaactct tttccgctgc tcagtccgtg agctggagca tcaatggcaa
     5221 taattgtttt tcctaaaggg agtaaatatg gtaataattt tttccaacga gaggaattgc
     5281 tttcccaacc gtgaattaat agaattatct cttcatttcc tctccacata taggtttgaa
     5341 aagtctcatt ttgaaaagag aaagtttctt tttcggtatt gaccaatgtt ttaggtaatt
     5401 tagttgaatc aattcttcct tttcgaggtt gactaaacaa ttgatacgct agctttttgg
     5461 ctttttcagg acgaacaaag cttagtatat tgacgtataa accaactgat tttaccagta
     5521 taaagtgttg tattcttttc ataaaaaaat cccgagaaaa tcgggattta aagtattaca
     5581 tttttgcgaa taatttttcc attttttctc tttcttcttc tgccaaagct gcatcaacaa
     5641 gaattcttcc tgaatgctca tcggttaata tttttttgcg agaagcaatt tcaacttgtg
     5701 tttgtggtgg aatagtaaag aaagaaccag ccgaagcacc acgttcaatc gatacaactg
     5761 ctaaaccatt gattacgcta ctacgaattc ttttgtaggc tgctaataag cgttcttcaa
     5821 tttttgcttc atattcagct gatttctgca ttaagaaatt ttcttctttt tgagtttcag
     5881 ccataatatc atttaattcg gcttttttat gttttaaatg agttgttttt tgatctaatt
     5941 tttctttaga atttgcaaca acttcttttt tatgttcaat agaagctttc atttctttaa
     6001 tgtgcttttc ggccaattga atttccaatt cttgaaactc aacttctttg gttaaagaat
     6061 tgaactctct attgttacga acattctttt gttgctctgt atatctttta atagaagctt
     6121 ggtgtgcttc gatagcattt tttttagctt ttatttggtc ttcaatcgaa accaaatcgg
     6181 ctttaaattt ttcaagacgc gtactcaaac ctgctacttc atcttctaaa tcttccactt
     6241 ccaaaggtaa ttcgcctcta acgtttctta tttcgtcaat tcttgaatcg ataatttgta
     6301 aatcatacag cgctcttaat ttgtcttcaa cgctcaattc ttttgtagta gccatatttt
     6361 ataagtactt aactgggttt gtattttctt ctgataaaac gattttcact atggaaaatg
     6421 caggtgcaaa attagtaatt ttttctttaa gaaaatcaac aatataattt tttgtatatc
     6481 tttcgctttc aaaatgtcct atatcggcta aaacgagttg attttcagct tcgtaatagt
     6541 tgtgatattt caaatcggct gttaaaaaaa catctgcatt agctagaata gcgtttttta
     6601 tagcaaaact tcctgaacca ccaagaacgg ctactttttt aattggtttt tcaactaatt
     6661 gtgaatgtct gatgtttcca cattgcattt tttctttaac aaaatgaaga aaatcttttg
     6721 cattcatagc tgtttctagt tcgccaatca ttcctaaacc aatattttga tgttcatttt
     6781 gcatgtgata gatttcataa gcaatttctt catatacatg attgctaaaa agtgctttca
     6841 gaatttttga ttgtaactgt ttttcaaagg ttacctcaat tttaatttct ttagcttcaa
     6901 taaactcaaa acgttcacca atttcgggat tactattttc atttcccata tatgttccta
     6961 taccttgaga attgaaacta caattttcat aatttccaat tttaccagca ccagcattga
     7021 ataacgcatt tctgagttgt acttcatttt caggaatagt ataggtaacc aatttttgaa
     7081 tgaaattttg ttttgggaca agaacttttg tgttaattaa accaagtgca tcacttaaaa
     7141 ttttgttgac gccatttgga tgattgtcta aagcagtatg taccgcataa atagcaatat
     7201 catttttaat agcttttaga acagcacgtt ctacatagtt tttgcctgtg attttcttca
     7261 aaccagaaaa aagaatagga tggaagcaaa ccactaaatt acactctttg gcaattgctt
     7321 cgtcaattac agcttccaac gcatcatgac acaccaatat tcctgttaat tcttgttctt
     7381 gatttccaac cagcaaaccc acattgtcaa actcttctgc ataagctaat ggtgctaatt
     7441 cttctagtat agggaaaagt tcttttactt tcatttgttt attttacagt gttttaatat
     7501 agaaaacgga acaccttatt aagtgctcca ttcattcaga gtttctataa ttgtccatat
     7561 agtattcttt ttttcaaata agtaaaaaca accactattg tgttcaatat ttcgataaaa
     7621 agctactttg actagacagt aatttttgga aaaatacatt ggtttgctaa caatcattaa
     7681 ccgttgattg ttttcaccat attgggttga aactttctga aaatcttcta aagaaaggca
     7741 atcattgact ttgcttttta aatattggtg ttgatcggta aacaatatat tgaattcttt
     7801 gctccaatct tcatttattg taatatcgat ttgcttttta attatttcag catttttttt
     7861 aagcaaatca tttttagaaa tgacttctaa tgtttcttca ttatttggtg ctttattgca
     7921 ataaaaattt aatagttgca aacgattttt aacaattact ttttcattaa cataggcttt
     7981 ggtaagaagt acttttaata tggccgcatt ttcaatttct tgtgaaaaac caagttgtat
     8041 tgtaataaaa aagagaaaag taaattttaa tttaaaggac atagataaga aatgataatt
     8101 aattccaaag ataaaacttt aaaaaaatat tcgtgcattc cattcaaatt atctactttc
     8161 gtactatgat tatcttaaga aaactacttt tcccttttgc tgttttatat ggaataatta
     8221 cttctattag aaattttcta tataatcaag gaattcttag aagttattct ttttccattc
     8281 ctcttatagc tgttggaaac cttagtgttg gtggaacagg caaaacacca caaatagaat
     8341 atttaatacg tttactttca aaagattata aagtagcggt tttaagcaga ggttataaac
     8401 gaaaatcaga aggttttgta ttaggaaata aaaattctac agctgaaatg cttggagatg
     8461 aaccttttca aatttttcaa aaatttccac aagttcaagt agcagtagat gttgatagga
     8521 aaaaaggaat cgagcaatta ttaaaattag aaaacaagcc tgaagttatt ttattagatg
     8581 atgcttttca acatcgtcgt gtaaaagctg gattttatgt tttgttaact gcttatgatg
     8641 atttatattg tgatgatttt atgttaccaa caggaaattt gcgagaaagc cgtaatggag
     8701 ttaaacgctc tgatttggtt gtggttacaa aatgtccaac ggctctttca aaagaagaaa
     8761 tgaagcgaat tataaagaaa ttaggaacaa caaaaaaagt tttttttagt acaattgctt
     8821 atgatgattt tgtttataat gaaaataact ctacatcaat atatgaaatt aaatcaaaag
     8881 aaaagttact tttggcagga atagcgaaac ctgcgttgtt ttttgactat ttgaaacaac
     8941 ccaatgatga cgtaatggct tttcctgatc atcatcattt taccgagaat gatataaaaa
     9001 atattaataa taaagctaaa aacaaaaaaa ttattactac tgagaaagat tatgttcgat
     9061 taaataacca cactttacaa tcggatattt attatttacc tataaaaagt aaatttctct
     9121 cagatacaga tgatttcaat aaaactataa aaaattatgt gggaacaagt acaagaaacc
     9181 gttaattaca ttaagagtaa gaccggatat acaccagatt atggtgttat tttaggctca
     9241 ggattaggaa gttttaccaa tgatattgct attgaattta caatgcctta caatgaaatt
     9301 ccaaattttc cagtatctac cgtcgaagga cacaaaggtg ctttggtttt tggaaaaatt
     9361 ggagacaaaa aagtagttgc catgcaaggt cgttttcatt attatgaagg ctattccatg
     9421 caagaagtta cgtttcctgt tcgagtgatg aaatatttag gaatcaataa actgattgtt
     9481 tcaaatgctt ctggtggtgt aaatccaaat tatcatgttg gtgttgttgc tataattacg
     9541 gatcatatta atatgtttcc cgagcatccg ttacgtggga aaaatgacac tcgttttgga
     9601 ccccgatttg taaatatgag cgaaccttac gatcgtaaaa tgattgtaaa agccaaagaa
     9661 ttggccaaag aacataattt agaagttttt gaaggtgttt atctcggctt acaaggacca
     9721 acatttgaaa cattagccga atataaaatg gtcatcaata tgggtgcaga ttgtgttgga
     9781 atgtcaactg ttcccgaagt gattgttgcc aaacacatgg atatggattg ctttggtgtt
     9841 tctgtaatta ccgatatggg tgatgttgat agtatcgatt ccatttcaca tgaagaagta
     9901 cttgaagcag ccaaaagtgc cgaaccaacg gttaggacat taattaagca actgattttg
     9961 aattactaag aactttcgaa attactgtat aaaaagtatt tatatcaaat ggtttggtaa
    10021 ttacatcatc cattccaaag cctaaaagta tgtctctatt ttcatttaaa gaaatagccg
    10081 ttaatgctat tacgggaagt gatttgttga actttcttat ttctcgtgtg gctattgtac
    10141 cgttaatact tggaagatgt acgtccatta aaattaaatc atagacattg ttgtttctga
    10201 tttcttcaat tgcgtcttcg ccattgtcta ttattttagt gatcatacct ttcttttcaa
    10261 ccattttttt agtaatcatt tggttgattt tgttgtcttc tacaataagt acatttttgc
    10321 cttccaacat tgtatcaatg tctgtatcta aaagtaatat ttcttctttt tctctatcaa
    10381 cttgattttc acttatttca aaagctaaat cgaaataaaa agttgaacct ttgcctaatt
    10441 cgctttcaag ttttatttcg ccacctaata aatcgataag tttttttaca attgccaatc
    10501 ctaatcctgt tcctccaaat tttctattaa tttcgattga accttgcgca aagctgtcaa
    10561 atatagtttc ttgcttgtct tttgcaattc caattccagt atccatcact gaaaaatgaa
    10621 tagtggcact attttctttt ataccaaata attctacaat tactttaact tcaccatttt
    10681 tggtaaattt taaggcattg ttgattaaat ttaaaaagat ttgtgacatt ttggttggat
    10741 cgccaataac tacattggga acattttcgt ctatatctaa tataaatttg ttgttattga
    10801 cttttgctaa ttcttttagt gagttttgaa tattgtttaa taaatctttt agttt
//
LOCUS       DAOOBH010000079        10554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_7927_length_10554_cov_5.172493, whole genome shotgun sequence.
ACCESSION   DAOOBH010000079 DAOOBH010000000
VERSION     DAOOBH010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7927_length_10554_cov_5.172493"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PK218_08375"
     CDS             complement(<1..1419)
                     /locus_tag="PK218_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW98562.1"
                     /translation="MTSSQFYNVLKNKFPFAPTVKQDIFFQQIAVFLTNTNSNELFLL
                     KGYAGTGKTTVISELVNNLFSIKKKYVLLAPTGRAAKVIANYSNKEAFTIHKKIYFPK
                     KSSNGSVGFTLKQNKHKNTVFIIDESSMISDTESDSKLYENGSLLDDLISYVYSGTDC
                     KMILIGDTAQLPPVQLDISPALNSDTLSMHYHKEVFSIELDEVMRQAEASGILYNATQ
                     LRELLKDSFITEFQFHLRGFKDIVRLTDGFDIQDAIESAYRNYSIEDTCFIVRSNKRA
                     NQYNQQIRTKILDKESDLSVGDFLMVVKNNYFWLKDSDEAGFIANGDIVEVLEIFAFK
                     ELYGFNFAKVKIRMVDYPNQKPFETIVLLNTITSESPSLTYEESNKLYQEVMKDYEGE
                     SQYRKFMKVKSNEYFNALQVKFSYAITCHKSQGGQWNTVFIEQPYLPDGITVDYVRWL
                     YTAITRAKDKLYLIGFKEESFEE"
     gene            1587..2399
                     /locus_tag="PK218_08380"
     CDS             1587..2399
                     /locus_tag="PK218_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HPW98563.1"
                     /translation="MWQNTSIIDKMYKKLAIQVSLSGLSFACFDTLTNKVISLEKIPL
                     EKFEKNTTIEELFSNAFLKYEELKAGYDQVVVLHSNNLSTFVPTPLFDEEYIGSYLQF
                     NTKVFETDFFAFDTLENYQMHNVYIPYVNMNNFFIDQFGTFDYKHANSILVEKILDLS
                     KNSYEHKMYIHVSETHFEIIVIRNQKLELYNSFEYKTPEDFLYYVLFTAEQLHLNPES
                     FPLELLGTIDEEHPLFKIAYQYIRNVSLLEVDATNNQFSEKELREHFILLHS"
     gene            2396..2944
                     /locus_tag="PK218_08385"
     CDS             2396..2944
                     /locus_tag="PK218_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPW98564.1"
                     /translation="MRIISGKYKGRRIQAPKNLPVRPTTDMSKEALFNILNNQFNFSE
                     VTILDLFAGTGNISYEFASRGSDTIVCVDGDMGCVNFIRKTAAEFEFDIVPIKSDVFA
                     FLEKHQESYDIVFADPPYDMAQKDFEKIPELVFKNNLVANEGVLVIEHSKHTKLDHLQ
                     HFSSARNYGGSVFSFFELKSEE"
     gene            complement(2946..3614)
                     /locus_tag="PK218_08390"
     CDS             complement(2946..3614)
                     /locus_tag="PK218_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPW98565.1"
                     /translation="MSAHIVKVLEARFITHDVKRFKVEKPEGYDFIPGQATVVSINLP
                     EWKDEIRPFTFTNLRDAKYLEFMIKIYSDHNGVTKLLGKVNAGDELIIHDVFGAIHYE
                     KPGVFIAAGSGITPFISIFRDLYNKNALRGNRLIYVNKTMDDIIMGEELFRMLKKDFV
                     NVLTRQNVIGYRERRIDRNYLIENIIDFSQHFYVCGPDDFVKEITTILLDLGAKSESL
                     IFEV"
     gene            complement(3643..3952)
                     /gene="rnpB"
                     /locus_tag="PK218_08395"
     ncRNA           complement(3643..3952)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK218_08395"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4057..4353
                     /locus_tag="PK218_08400"
     CDS             4057..4353
                     /locus_tag="PK218_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98566.1"
                     /translation="MEKSYHYASVIKALEELKAKGFTLDFNLQEEVIIQNPNDYEIIH
                     IYRYEGESNPDDSAVVYGIQSKHGENGVFVAGYSANSESQAAKVLREISIKRKL"
     gene            4459..5541
                     /gene="dnaX"
                     /locus_tag="PK218_08405"
     CDS             4459..5541
                     /gene="dnaX"
                     /locus_tag="PK218_08405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPW98567.1"
                     /translation="MEQFVVSARKYRPQTFKDVVGQQAITNTLLNAIESNHLAQALLF
                     TGPRGVGKTTCARILARKINQPGYDDPLEDFAFNVFELDAASNNSVDDIRNLIDQVRI
                     PPQTGQYKVYIIDEVHMLSASAFNAFLKTLEEPPKHAIFILATTEKHKIIPTILSRCQ
                     IFDFKRITVKDAREHLAEVAKSQNVTYEEDALHIIAQKADGAMRDALSIFDRVVSYCG
                     NNLTRQAVTENLNVLDYETYIEITNLLLENKIPETLMMYNDVLAKGFDGHHFVAGLAS
                     HFRDLMVTKNPSTLSLLEVGEQAQELYRLQSQKAPQDFLLKGIELANDCDLKFKVSQN
                     QRLLVELCLMQLCSITLDGEKKKLTS"
     gene            5685..6209
                     /locus_tag="PK218_08410"
     CDS             5685..6209
                     /locus_tag="PK218_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98568.1"
                     /translation="MLKVEESATIKTETKVSDGFTSTKVSAFSLKSISIKKELAENQK
                     NISKDTSHLPSEIFTETEMQEQWFKYAQRLSDKGHMIMMSLLSINEPTLEGTTIIHEL
                     PNEGSKIDFEREMPELLGYLRGKLHNHDIKITVRINEAIEAKKAFTPQDKYNRMLEIN
                     PNLEILKKMFDLEL"
     gene            complement(6206..7096)
                     /locus_tag="PK218_08415"
     CDS             complement(6206..7096)
                     /locus_tag="PK218_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HPW98569.1"
                     /translation="MITIKKYAAIDIGSNAMRMLISNIVEQQGKETQFNKSALIRVPI
                     RLGQDAFTVGEISEENIDRMVDAMKAFKLLMKVYRIEKYKACATSAMREAFNGKEVAD
                     YIYQQSDIKIDIIDGKNEAAIIASTDLHSFIKTDQAYLYVDVGGGSTEFSLFNDGKMI
                     ASKSFKNGTVRLLNDMVNEIVWTEIEKWIKEKTTDLENVILIGSGGNINKIFKMSGKS
                     QEQPLTYLYLNAQYQYLNSLTYEQRIAELGLNTDRADVIIPATKIYLNAMKWSGATKI
                     YVPKIGLSDGIIKAMYQEII"
     gene            complement(7103..9244)
                     /gene="ppk1"
                     /locus_tag="PK218_08420"
     CDS             complement(7103..9244)
                     /gene="ppk1"
                     /locus_tag="PK218_08420"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPW98570.1"
                     /translation="MTVEKNYTYIDREKSWLAFNARVLQEAADDTVPLLERLRFLGIF
                     SNNLDEFFRVRFAAVRRLSLSGVTGEKLLGGITAQQLVKDITKIVIKQQNESLKVLGE
                     IEKQLEKQNIIIVDEKQVTQTQEEYIKDFFIQKMSQELVTIILNDLAEFPLLVDNTGY
                     LTVRLVMKNEDKPASVLDIVKKKKKEIRYALIEIPKSFNRFLVLPKKDNKQYVILLDD
                     VIRLNLSSIFNIFDYESISVHMIKITRDAQLDIDSDMSKSMLEKIATSVKDRRIGEPV
                     RFVYDQSIEKDTLNFFLTKMGIELTDSIIPGGKYHNRRDYMSFPDLGRQDLLYQQQDP
                     LPVEGLSLDGSILNRIAKKDYLIHTPYQSYSYLIKFLREAALDPKVTSIKITLYRLAK
                     NSQIISSLINAAKNGKKVTVQIELQARFDEASNINYAEVMQQEGIELIFGIKGLKVHS
                     KICVIERIEKEKIKRYGFISTGNFNESTAKIYTDLTLFTCHQQILKDVNKIFEFFDVN
                     YRIHRYKHLIVSPHYTRSKFNKLIDREIQNAQMGRPAYLNLKMNSLSDYPIIDKLYDA
                     SNAGVKVKLQVRGICSLIPGVKGMSENIEAISIVDNFLEHSRAYIFCNNDNPEVFISS
                     ADFMRRNLDGRVEVTCPIYDEDIKKAIIDTFDIGWRGNVKARIHSADLENKYKARTTN
                     ESIFRAQFETYNYFLDHNSEVLNTNCIVPEK"
     gene            complement(9248..9733)
                     /locus_tag="PK218_08425"
     CDS             complement(9248..9733)
                     /locus_tag="PK218_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPW98571.1"
                     /translation="MKQLILVRHAKSSWDLPVQDFDRPLSNRGIQDAHLISSKVINYL
                     PETFIIWSSAAKRAMETAIIFTQNFSCPIESIIFKKELYTFDLTKLETIIKNCNDQFD
                     NLILFGHNEAITHFVNKFGNVTVDNVSTSGFVSITFETNSWKAISNGKTNKIILPKDF
                     N"
     gene            10292..>10554
                     /locus_tag="PK218_08430"
     CDS             10292..>10554
                     /locus_tag="PK218_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98572.1"
                     /translation="MKKVNPNPLINRQTTYLFDKKCETMSKKVILLFMFVMFITNISA
                     QVTNDEDAPVIREISTFINQGALMRSAKPGYSDSKNLENLVTKV"
BASE COUNT         3510 a         1858 c         1683 g         3503 t
ORIGIN      
        1 ctcctcaaaa ctttcctctt taaacccaat taaatacaat ttgtctttgg cacgtgtaat
       61 ggcggtgtac aaccagcgaa cgtaatctac agtaatgcca tcgggcagat agggttgttc
      121 tataaaaacg gtgttccatt gtccgccttg gcttttgtga caggtgatgg cgtaagagaa
      181 ttttacttgc aaggcgttga agtattcgtt gctttttacc ttcataaact ttcggtattg
      241 cgattcgcct tcgtaatctt tcatcacttc ttgatagagt ttgttacttt cttcataagt
      301 taatgatggc gattcgctgg taatggtatt gagtaatacg atggtttcaa atggtttttg
      361 attgggataa tctaccattc taatttttac tttggcaaaa ttaaatccgt ataattcttt
      421 gaacgcaaag atttctaata cttctacgat gtcaccattg gcaataaacc cggcttcgtc
      481 ggagtctttg agccagaagt aattgttttt tacgaccatc aaaaaatcgc caacagagag
      541 atcactttct ttgtcaagaa ttttggttct aatttgttgg ttgtattggt tggctctttt
      601 gttagagcga acaataaagc aggtgtcttc tatgctatag tttcgatacg cactttctat
      661 ggcatcttga atatcaaaac catctgtcaa acgcactatg tctttaaagc cacgaaggtg
      721 aaactgaaac tcggtaataa aactgtcttt caacaactcg cgcaattgtg tggcattgta
      781 taaaataccc gatgcttcgg cttgtcgcat tacttcatct aactcgattg aaaacacttc
      841 tttgtgataa tgcatagaca aagtatcgct attcaatgcc ggactaatat ctaattgtac
      901 tggtggcaac tgcgccgtat cgcctatcaa aatcatcttg caatcggtgc cactatagac
      961 gtaggaaatc aaatcgtcga gcaacgaacc gttttcatac aacttcgaat cggattcggt
     1021 atccgaaatc atggacgatt catcaatgat aaatactgtg tttttgtgtt tgttttgttt
     1081 tagcgtaaaa ccaaccgaac cgttgctgct tttctttgga aaatagattt ttttgtgaat
     1141 agtaaaagct tctttgttgg aataattggc aatcactttg gccgctctac ccgttggtgc
     1201 cagcaagaca tatttttttt tgatagaaaa taaattatta accaactccg aaatgacggt
     1261 cgttttccca gttccggcat atccttttag aagaaatagt tcgttggaat tggtattggt
     1321 tagaaaaaca gctatctgct gaaaaaaaat atcttgtttt acagtaggcg caaaaggaaa
     1381 tttgtttttt aaaacattgt aaaactggct tgaagtcatt actgtattta atataaaaga
     1441 acttcaaaga tacgcataga tatgataata caaattaata caaaccattt atatactcag
     1501 tgattttaga tttgaaataa attattctat tgcaattgaa tttgaatttc gtaagtttgt
     1561 tttttttaaa aaaaggcaac caaactatgt ggcaaaacac cagcataatc gataagatgt
     1621 ataaaaagtt agcaattcaa gtctcattaa gcggtctgtc gtttgcttgt ttcgatactc
     1681 ttaccaataa agtcatttct ttagaaaaga ttcctttaga aaaatttgaa aaaaacacca
     1741 ccatagaaga actgttcagt aatgcctttc tgaagtacga agaactcaaa gcaggatacg
     1801 accaagtagt tgtgctacac agcaacaatt tatcaacctt tgttccaaca cctctttttg
     1861 acgaagaata catcggaagc tatctccaat tcaacactaa agtctttgaa accgatttct
     1921 ttgcctttga cactttagaa aactatcaaa tgcacaatgt ttatatacct tatgtaaaca
     1981 tgaataactt cttcatagac caattcggaa cctttgatta taaacacgcc aacagtattc
     2041 tagtagaaaa aatccttgat ttatcaaaga acagttacga acacaagatg tacatccatg
     2101 ttagtgaaac tcactttgaa atcatcgtaa ttagaaatca aaagctagaa ctctataatt
     2161 cttttgagta taaaaccccc gaagactttc tatattacgt cttattcacc gcagagcagt
     2221 tacatctaaa cccagaaagt ttccctttag aactcctagg aactatagac gaagagcatc
     2281 ctctattcaa aatagcatac caatacatcc gaaatgtttc actactagaa gttgatgcta
     2341 ccaacaatca attctccgaa aaagaattaa gagaacattt tatactgtta cactcatgag
     2401 aataatttct ggaaaatata aaggaagaag gattcaagca ccaaagaatt tacctgttcg
     2461 cccaacgacg gatatgagca aagaagcctt gtttaacatc ttgaacaatc aatttaactt
     2521 cagtgaagtg accattcttg atttatttgc cggaacagga aacattagtt atgagtttgc
     2581 ctcacgtggt agcgatacca ttgtttgtgt agatggtgat atgggttgtg tcaacttcat
     2641 ccgtaaaacc gctgccgaat ttgagtttga tattgttcct ataaaatcgg atgtatttgc
     2701 ctttttagaa aaacaccaag aaagttacga tattgttttt gccgatccgc cttatgatat
     2761 ggcgcaaaag gactttgaaa aaattcccga attggttttt aaaaacaacc ttgttgctaa
     2821 tgaaggtgtc ttagttattg aacactccaa acataccaag ttagaccatt tacaacattt
     2881 ttcttctgcc agaaattatg gtggttcagt atttagtttc tttgaattaa aatcagaaga
     2941 ataatttata cctcaaagat gagtgattct gattttgctc ccaagtcgag taagatagta
     3001 gtaatctctt ttacaaaatc atctggacca caaacataaa aatgttgact aaaatcaatg
     3061 atgttttcaa tcaaatagtt tcggtcaatt ctgcgttctc tatatcctat tacgttttgt
     3121 ctagtaagca cattcacaaa atctttcttg agcattcgaa ataattcttc acccatgata
     3181 atatcgtcca tggttttatt tacataaatc aaacgattac ctcgaagcgc attcttattg
     3241 tataaatctc taaagatgga tataaaaggt gttataccag aacccgcagc aatgaaaaca
     3301 cctggttttt cataatgtat tgcaccaaaa acatcatgaa taatgagttc atcacctgca
     3361 tttactttac ccaacaactt cgttacaccg ttgtggtcac tataaatctt aatcataaac
     3421 tcaagatact ttgcatctct aagattggta aaagtaaaag gtcttatttc gtctttccat
     3481 tctggaagat ttatagaaac tacagtagct tgtcctggaa taaaatcata tccttcaggc
     3541 ttttcaactt taaatcgttt tacatcatgc gttataaaac gagcttccaa tacttttaca
     3601 atgtgagcac tcatagtatt ttaattaatt ttgaatatat taaaaaaaag cagacctata
     3661 agccggattc tgtatccgcc gaggcggact cttatcattt atctagacca acaattactc
     3721 gttggttcaa gcttcctacc cttcgacaac ggacgagtaa tccttaaatg ccgatatact
     3781 tggaatttca ccgcatagag tttacctggt ttcactacag cattacctgt acctgctttc
     3841 tgttgcactt gtccttccgc tgtggcggtg acggatgtta tccgttatgc tactctgtgg
     3901 tgtccggact ttcctctccc aattactcag gaacgataag acggtctgcg gaacaaaggt
     3961 agaattttaa attttagata ttaaatattg atattagata tttcttaaga cttttgagtt
     4021 tttgattttg tatatttata aataaataat caaatcatgg aaaagagtta tcactacgca
     4081 tctgtaataa aagcattaga agaattaaaa gctaaaggat ttacgcttga cttcaatctt
     4141 caagaagaag ttatcataca aaatcctaac gattatgaaa tcatacacat atatagatat
     4201 gaaggagaat ccaatcctga cgattctgct gtagtttacg gcattcaatc taaacatggt
     4261 gaaaatggtg tctttgtagc aggttattct gccaattccg aaagtcaagc tgctaaagtt
     4321 ttgagagaaa tcagtattaa aagaaaacta taatcgttac atcttattca caaagtgctg
     4381 aaaacttttc atcaaatatg tgcttaacca atttatcaaa ccaacaaaaa ttatatattt
     4441 gtaatccaat aaaaacctat ggaacaattt gtagtatcgg cgcgcaagta tcgtccgcaa
     4501 acgtttaaag acgtagtggg acaacaagcc attaccaata cattattgaa tgctattgaa
     4561 agtaatcact tagcgcaagc attacttttt acaggtccaa gaggtgttgg aaaaactacc
     4621 tgtgcccgaa tattagcccg aaaaataaac caaccaggct atgacgatcc gctagaagac
     4681 tttgcattta acgtatttga attggatgcc gcatctaata actcagttga tgacatccga
     4741 aacctgattg accaagtacg cataccacca caaaccggac aatataaagt atatataatt
     4801 gacgaggtac acatgctgtc ggcttcggct ttcaatgctt ttttgaaaac attagaagag
     4861 ccaccaaaac atgccatctt tatattggca accaccgaaa aacataaaat cattccaact
     4921 atattgtctc gttgtcaaat atttgatttt aagagaatca ctgtaaaaga tgcgagagaa
     4981 cacttagccg aagttgctaa gagtcaaaat gtaacttatg aagaagatgc tttgcacatc
     5041 attgcccaaa aagccgatgg tgctatgcgt gatgcattat ctatttttga ccgtgtggtt
     5101 tcctattgtg gtaataactt aacccgtcaa gcggttacgg aaaacttgaa cgtgttggat
     5161 tatgaaacct atatagaaat taccaactta ctactagaga ataaaattcc tgaaaccttg
     5221 atgatgtaca acgatgtgtt ggctaaaggt tttgacggac atcacttcgt tgcaggattg
     5281 gcttctcact ttagagattt gatggttacc aaaaacccaa gcactttgtc gttgcttgaa
     5341 gttggagaac aagcccaaga actataccga ctacaatcgc aaaaggcacc acaagatttt
     5401 ttgttaaaag gcatcgaact ggctaatgat tgtgatttaa aattcaaggt gtcgcaaaac
     5461 caacgattac ttgtcgaact ttgtcttatg caactttgct ctatcacact tgatggagaa
     5521 aaaaaaaagc taacttcata attccaccta tctattttaa aaaccaaggc tattctatag
     5581 ccgaaaaggt taccaaagct gaggttacaa ttgcgattcc tgacgctaaa gtttcaacat
     5641 caaaagtaaa cgttgttagt gctgaaattg aagttcccga aaccgtgcta aaagttgaag
     5701 aaagcgctac tataaagacc gaaactaaag taagtgatgg ttttacttct accaaagttt
     5761 ctgctttttc gctgaaaagt atttcaataa aaaaagaatt agcagagaat caaaagaata
     5821 tttctaaaga taccagccac ctgcctagtg aaatttttac ggaaacagaa atgcaagaac
     5881 aatggtttaa atatgcacaa cgactaagtg acaaaggtca catgattatg atgtcattat
     5941 tgagcatcaa cgaacctact ctagaaggta caactattat tcacgaattg ccaaacgaag
     6001 gctctaaaat tgattttgaa cgcgaaatgc ccgaattact gggttacttg agaggaaaac
     6061 tacacaacca cgacatcaaa atcactgtta gaatcaacga agccatcgaa gctaaaaaag
     6121 catttacgcc gcaagacaag tacaatagaa tgcttgaaat caatcctaat ttagagatat
     6181 tgaaaaaaat gtttgattta gaactctaaa ttatctcttg atacatcgct ttaatgatac
     6241 cgtccgaaag accaattttt ggcacgtata ttttagtagc accactccat ttcatagcgt
     6301 taagatatat cttggtagcc ggaataatta catcggctct atcggtgttt agtcccaatt
     6361 cggcaatgcg ctgttcataa gttagcgagt ttagatattg gtattgcgca tttaaatata
     6421 aataagttag cggttgttct tgtgatttgc ccgacatctt aaatatcttg ttgatgtttc
     6481 cgccagaacc aatgagaata acgttttcta aatcggttgt tttttcctta atccactttt
     6541 ctatttcggt ccaaacaatt tcgtttacca tatcgtttaa caaacgaaca gttccgtttt
     6601 tgaaggattt tgaagcaatc attttgccat cattaaagag tgagaactcg gtacttccac
     6661 caccaacatc aacatagaga taggcttggt ctgttttaat aaacgaatgc aaatcggtag
     6721 aagcaatgat agcggcttca ttttttccgt caattatatc aattttaatg tctgattgtt
     6781 ggtaaatata atcagctact tctttaccat taaaggcttc gcgcattgcc gatgtggcac
     6841 aggctttgta tttttcaatt cgataaactt tcattaagag tttgaaggct ttcatagcat
     6901 caaccattct gtcgatgttt tcttcggata tttctccaac ggtaaaagca tcttgcccaa
     6961 gacggatagg cacacgaatc agtgcgctct tgttaaactg agtttccttt ccttgttgtt
     7021 ctacaatgtt cgatatgagc attcgcatgg cattggaacc aatatcgata gcagcatatt
     7081 tcttaatggt tatcatgctt atttattttt cgggtacgat acaatttgtg tttaacactt
     7141 cactattatg gtcaagaaaa taattatagg tttcaaattg agctctaaaa atagattcgt
     7201 ttgtagttct tgctttgtat ttattttcca aatccgcaga atgaatccga gctttaacgt
     7261 ttccacgcca accaatatcg aaagtatcaa taatagcttt ctttatatct tcatcataaa
     7321 tcggacaggt tacttctact ctaccgtcaa gatttcttcg cataaaatcg gctgaagaaa
     7381 tgaatacctc gggattatca ttattgcaaa aaatatatgc tctcgaatgt tccaagaaat
     7441 tatcaacaat gctaatagct tctatgtttt cgctcattcc tttaacacct gggattaatg
     7501 aacagatacc tcttacttgt agttttacct tcacaccagc gttacttgcg tcgtataatt
     7561 tatcaataat tgggtaatct gacaaactgt tcatctttaa atttaagtat gctggtcgtc
     7621 ccatttgagc attttgaatt tcacgatcta tcaatttatt gaactttgaa cgagtataat
     7681 gtggcgaaac aatgaggtgt ttgtaacgat ggatgcgata gttaacatca aagaattcga
     7741 aaattttgtt gacgtctttt aaaatctgtt gatggcaagt aaacaaagtt aaatcggtat
     7801 aaattttagc tgttgattcg ttaaagtttc ctgtagaaat aaatccgtac cgtttgattt
     7861 tttctttttc gatacgctca ataacacata ttttactgtg tacttttaat cctttgatac
     7921 caaaaatgag ttcgataccc tcttgttgca ttacttcggc ataatttata ttagaagcct
     7981 catcaaatct agcttgaagt tcaatttgta cagttacttt ttttccattt ttagctgcat
     8041 ttattaagga actaataatt tgcgagtttt tggccagtcg gtataatgtg attttaattg
     8101 aagttacctt tggatcaaga gcggcttctc gcaaaaattt tattagataa gaataagact
     8161 gatacggtgt atgtataaga taatcttttt tagcaattct attcaaaata ctaccatcaa
     8221 gagacaatcc ttctactggc aacggatctt gttgttggta taataagtct tgacgaccta
     8281 aatcgggaaa tgacatataa tcacgacggt tatggtattt tccaccaggt atgatgctat
     8341 cggtaagttc aatacccatt ttggttaaaa agaaattgag cgtatctttt tcaatcgatt
     8401 ggtcgtaaac aaatcgaaca ggttcgccaa ttcgtctatc ttttaccgat gtagctattt
     8461 tttcgagcat acttttactc atatcgctat caatgtctaa ttgtgcatcg cgagtaattt
     8521 ttatcatgtg taccgaaata ctttcgtaat caaaaatgtt aaaaatactg ctcaaattca
     8581 atcgaataac atcatcaagc agtatcacat actgtttatt atcttttttg ggaagcacta
     8641 aaaatcgatt aaaagacttt ggaatttcga ttaatgcata acgaatttct ttttttttct
     8701 tttttacaat atctaaaaca gaagctggtt tatcttcatt tttcatcacc aatcgaacgg
     8761 ttaaataacc cgtattgtca actaacaaag gaaattcagc caaatcattt aagataatag
     8821 taaccagttc ttgactcatt ttttgaataa aaaagtcttt gatatattct tcttgtgttt
     8881 gggtaacttg tttttcatca acaataatga tgttctgttt ttcgagttgt ttttcaattt
     8941 cgcccagcac ttttaaactt tcgttttgct gtttgataac gattttagta atgtctttta
     9001 ctaattgttg tgccgtaatt ccgcctaata acttttcacc agtaacaccc gaaagtgaaa
     9061 gtcttctaac cgcagcaaat ctcactctaa aaaactcatc taagttgttg gaaaatattc
     9121 ctaaaaaacg aagtctttct aacaatggaa cggtatcgtc tgcggcttct tgtaaaactc
     9181 tagcattgaa agcgagccaa cttttttctc tatctatata ggtataattt ttttctacag
     9241 tcataaatta attaaaatct tttggaagaa taattttatt tgtttttccg ttagaaattg
     9301 ctttccaaga atttgtttca aaggtaatag aaacaaagcc tgaagtagaa acattatcaa
     9361 cagttacatt accaaattta ttaacaaaat gtgtgattgc ttcgttgtgt ccaaaaagta
     9421 ttagattatc aaattgatca ttacaatttt ttattattgt ttcaagctta gttaaatcaa
     9481 aagtatataa ctctttttta aaaataatac tttcaatagg acatgaaaaa ttttgagtaa
     9541 aaataatagc cgtttccatt gctctttttg ctgcactaga ccatattata aaagtttctg
     9601 gcaaataatt tattacttta cttgaaatta aatgtgcatc ttgaattcct ctattagaca
     9661 gtggtctgtc aaaatcttga acaggcaaat cccaacttga tttggcatgt ctcactaata
     9721 ttaattgctt catgagatta ttagtaatag attataaata aatttaattt cataaatata
     9781 ggaatttgaa attgaatatt cagcaacaac ataatattta taaaatataa catcaatact
     9841 ttagaattaa attgtttgtt attaatattt ttttgtttaa aattcataga aacttacaaa
     9901 tgttataaac aatacgatgt taatatattt ttgtagcttt tttactttta taaaataaaa
     9961 aatatgcatt ttatctatgt ttttatgcat tttatcgaca tttttctttg tcaattaaaa
    10021 aagataatat actttcgacc tataatttaa ctaaaaacca agaaaaatgt ttgaaaaaaa
    10081 tttaaaaaac aaccaaaaaa aatccgatta aatgaataaa atacgtcaaa atcaacattt
    10141 catcgagttt tctagctatt gatagaaaaa attaatcatt atgagaacat gtatctaaat
    10201 tagctccaaa attaatgtta aaaataaata gcaataatga gtagaaaaaa aattacatga
    10261 ttgaaaagta atgaattaaa aaattattat catgaaaaaa gtgaacccaa accctttaat
    10321 taaccgtcaa acaacctatt tgtttgataa aaaatgcgaa actatgagta aaaaagtaat
    10381 attacttttt atgtttgtta tgtttataac aaacatatct gcacaagtta ccaatgatga
    10441 agatgctccg gttatccgag agattagtac attcattaat caaggtgcct tgatgcgttc
    10501 tgcaaaacct ggttattctg attcaaaaaa tttagaaaac ttggtaacca aagt
//
LOCUS       DAOOBH010000080        10408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_8043_length_10408_cov_5.025693, whole genome shotgun sequence.
ACCESSION   DAOOBH010000080 DAOOBH010000000
VERSION     DAOOBH010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8043_length_10408_cov_5.025693"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PK218_08435"
     CDS             <1..223
                     /locus_tag="PK218_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-binding protein"
                     /protein_id="HPW98573.1"
                     /translation="INNDGTFSATDSENILDSGTVTITEGKIEKNGAVTPGGHTVDKI
                     SFRAHYSYDAPGYDILYEGYKRTGFLEDE"
     gene            293..1027
                     /gene="rlmB"
                     /locus_tag="PK218_08440"
     CDS             293..1027
                     /gene="rlmB"
                     /locus_tag="PK218_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPW98574.1"
                     /translation="MKDEQQIFGIRAIIEAIQSGTVIDKVFIQKEVQGDLMKELMKVM
                     KQKSINYSYVPVEKLNRLTSNNHQGAVATISPIGFIEIETLIETVMENGRKPFFLILD
                     QLSDARNFGAIIRTAECTGVDGIIIQKQGSAPVNGDTVKTSAGAVFNIPICKVDHIKD
                     AIFHLQANGIKTVAATEKTEQNIYDISLNEPLAIIMGSEDRGINPSVLKIVDEKAKLP
                     MFGTIGSLNVSVACGAFLYETVRQRQ"
     gene            complement(1038..1805)
                     /locus_tag="PK218_08445"
     CDS             complement(1038..1805)
                     /locus_tag="PK218_08445"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPW98575.1"
                     /translation="MDEQNHFKFTPSVWIVPTLFLLIIWTVFLIEDTFNLKLNPYGIF
                     PRTFSGMKGIVLSPFLHGDFNHIASNSVPLFVLTLALIYFYRNLSLKVLVYGILLSGT
                     ITWIIGRNSYHIGASGLIYVLVSFIFFKGMMTKYYRLMALSLTVVMVYGGMVWYVFPE
                     VDAKISWEGHLAGLLTGFILAAYYKTPDYVKSIQYDWEKPEFVAENDAFMKRFDENGN
                     FVNPPKEEIIEVLETFSPEIKYVYEYIETPKNESSND"
     gene            1890..3167
                     /locus_tag="PK218_08450"
     CDS             1890..3167
                     /locus_tag="PK218_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPW98576.1"
                     /translation="MEIPLAERIRPQKLEEYISQLHLVGDNGSLTQQIKRGIIPSMIF
                     WGSPGTGKTTLAQIIAKQSNRPFYELSAINSGVKDIRDVIEKAKQSGGLFTAKNPILF
                     IDEIHRFSKSQQDSLLAAVEKGWITLIGATTENPSFEVIPALLSRCQVYVLNSFSKDD
                     LIALLHRAMKEDKIISNKKIKLKETEALLRLSGGDGRKLLNIFELVINASSEGQIIIT
                     NKKVMELVQQNTVLYDKTGEQHYDIVSAFIKSIRGSDPNGAVYWLARMIEGGEDVKFI
                     ARRMLILSSEDIGNANPTAFIMANNTFQAVSIIGYPESRILLSQCAIYLATSPKSNAS
                     YMAINEAQQVVKQTGDLSVPIHLRNAPTKLMKELGYGEEYQYSHNYANNFSEQEYLPD
                     EISTTAFYKPGNNARENGTREFLKNRWKDKYDY"
     gene            3236..3637
                     /locus_tag="PK218_08455"
     CDS             3236..3637
                     /locus_tag="PK218_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98577.1"
                     /translation="MKIIYNEKDKTIEINDGLKSHFLLIKILMLLILINSVINLIGIA
                     KTGLGFMEIIWLFLGTISVVVLYSLYFKESSLEKIPITKIKRLMGKTSPTKKQYYFEL
                     VDGKKRVLKELKTDKDSKELRKLLTKLGIEN"
     gene            complement(3639..4364)
                     /locus_tag="PK218_08460"
     CDS             complement(3639..4364)
                     /locus_tag="PK218_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98578.1"
                     /translation="MKKIALLFLLTVFNVFSQNINDYKFVIVPTKFDFLKEDNSFNLN
                     TSTKLLLEKYGFEVFMNNQELPTEIANNRCKTLTASLAKDNSMFQTKIKLVLKDCQDR
                     IVYETAFGTSREKDYNKAYNQAFREAAKSFETLNYKYNGEKQIAVEKIEENQEVKSVE
                     PYSLSSENSEIFYFAQPIANGFQVVNNEPRVIMRLFTTSQKGVFIGIKGDQNGVVITK
                     NGKWFFECYEGGKLTSEPINLRF"
     gene            complement(4367..5053)
                     /locus_tag="PK218_08465"
     CDS             complement(4367..5053)
                     /locus_tag="PK218_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HPW98579.1"
                     /translation="MMNNITDIFFDLDHTLWDFDKNSIQAFDKIFKKQYPTIDTVVFM
                     EKYVPINQACWKLYQFDKMTHEELRYQRLKQSFDAINFAISDAEINQISHDYIEFLPD
                     NNLLFDGAMEVLEYLKPKYTLHIITNGFAEVQTKKLNNSGINDYFKTVTNSEIAGVKK
                     PHPNIFEFALSLTSVSKENALMIGDCIDADIRGAINFGIKAILFDQANSHNTNEVQKI
                     NHLLELKKIL"
     gene            complement(5053..5745)
                     /gene="radC"
                     /locus_tag="PK218_08470"
     CDS             complement(5053..5745)
                     /gene="radC"
                     /locus_tag="PK218_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPW98580.1"
                     /translation="MEPINFSIKNWAEDDKPREKLMLKGKQALSDAELIAVLIGSGSR
                     NESAVDLSKRILKSVDNNLNALGKLSLKQLMEFKGIGEAKAISIAAAMELGRRRREEE
                     TVELKKITSSKAVFEIMQPIIGELPHEEFWVLYLNNSNKVIYKAQLSKGGITGTVVDV
                     RLIYKTALEQNATSIILSHNHPSGKLQASDADLQITKKLKAAGEQLDIKVLDHIIITE
                     NGYLSFQDEGIF"
     gene            5797..6378
                     /locus_tag="PK218_08475"
     CDS             5797..6378
                     /locus_tag="PK218_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HPW98581.1"
                     /translation="MKPISFLLLLCSFLTTITKTFEEKLSDAAISIIDSSIVYTPNYV
                     AIKYPNGDVPAKTGVCTDVVIRAYRKLGIDLQKEVHEDMKANFSKYPNFKKWGIKTCD
                     TNIDHRRVPNLEVFFERKGQKLAVTQNANDYKTGEIVTWLIANKYPHIGIITNKKSSD
                     GKRPLIVHNVGGGQILEDCLFSWEIVGHYSYEK"
     gene            6430..7926
                     /locus_tag="PK218_08480"
     CDS             6430..7926
                     /locus_tag="PK218_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98582.1"
                     /translation="MKKIYIFIFLFIVVNVFSQQAGELDLNFGNGGFFTTENNSDIDN
                     ANCLFVSESDEIYVAGGNDNFSMIKLMADGTYDSAFGNQGQVIIPFDGFRAEAKEIIV
                     RADGKIILAGNVYVSGGEYNFGIVLLNTDGSLDTSFNEIGKLIFDFDENINYLYAAAI
                     QNDGKIVVAGQCGTFANADFAVARINPDGTFDTEFGVGGKQRINIQGDDRGRCVVIQN
                     DGKIVLGGYSYPVGGNNCWFAAVRLTTTGQLDNTYSIDGKAVAKVASSYGNDTVQDMI
                     IQPDDKILMVADSYIGSSQDIGMVRFTTDGELDTTFSGDGKFNTSMIGTTDYPRAITL
                     QTDGKILISGSFYTTPQRNLALLRLTANGELDTTFSQDGKANYIVPGNNGVSVGDMKT
                     QSTGQILICGSIFNNYMVARIFSGSELNTNEYELKRLFFYPNPASKTIYFSENVKSVN
                     VFTLEGQKIMETINFPAQMDISTISKGIYILNFQTVYGKNINEKLIVE"
     gene            complement(7982..9337)
                     /locus_tag="PK218_08485"
     CDS             complement(7982..9337)
                     /locus_tag="PK218_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPW98583.1"
                     /translation="MRTHFIAIGGAAMHNLAIALHNKGYKVTGSDDAIFEPSKSRLEK
                     KGLLPIEMGWFPEKITSDIEAIILGMHAKADNPELLKAKELGLKIYSYPEFLYEQSKN
                     KTRVVIGGSHGKTTITSMILHVMHFQNIEVDYMVGAQLEGFDTMVHLTEENDFIVLEG
                     DEYLSSPIDRRPKFHLYQPNIALLSGIAWDHINVFPTFENYLEQFEIFVNQIIKGGIL
                     VYNEEDKTVKKVAEETTNTIRRLPYQTPNYKVEDGTTLLETPEGFMPIEVFGAHNLNN
                     LAGAKWICQNMGVDEAEFYEAIASFKGASKRLEKIAEGKGKVAYKDFAHSPSKVSATA
                     KAVKNQYPDRKLVACLELHTYSSLNAEFLKEYEGALDAADVAVVFYSPDAVKIKQLEE
                     ISYNQIANAFQRDDLIIYTNPAAFKEFIFNYDLNNSALLLMSSGNYGGLNFDEVKQLV
                     E"
     gene            complement(9407..10096)
                     /locus_tag="PK218_08490"
     CDS             complement(9407..10096)
                     /locus_tag="PK218_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW98584.1"
                     /translation="MKLFINIGLLILIFISNDIDFQKAENYFKAEKYSLAKPIFEKIL
                     KEEPNNLKTIEYLGDIAGIEKKWEDAITYYEKLKKSNPNNANYWYKFGGAMGMKAKSV
                     NKFKALGMIDDVENAFLKATQLDSKHIDSRWALVMLYIELPGIIGGSERKAQKYSNEL
                     MKLSKVDGYLSQGYIDEYFDRYKKAETNYLKAYQIGNSKTTFQKLYDLYQNKLKDKAK
                     AIKLKEQFEKK"
BASE COUNT         3473 a         1667 c         1694 g         3574 t
ORIGIN      
        1 aataaacaat gatggaacgt ttagtgctac tgattccgaa aatattttag actcgggtac
       61 agttacaata accgaaggga aaattgaaaa aaatggtgca gttactcctg gaggacatac
      121 tgtagataaa atttctttta gagctcatta tagttatgat gcccctggat atgatatact
      181 atatgaaggt tataaaagaa ctggtttctt agaagatgaa taataaaaaa atcattgtat
      241 atttagcaaa accatcccac tttcgggatg gtttttttta tacctttaca ccatgaaaga
      301 cgaacaacaa atatttggaa ttagagccat cattgaagcc atccaatcgg gaactgttat
      361 tgataaagta tttatccaaa aagaggttca aggagatttg atgaaagaat tgatgaaagt
      421 gatgaaacaa aaaagcatca actattccta tgttcctgtt gaaaaactca atcgtttaac
      481 ttctaataat catcaaggtg cagtggctac catctcacct attggtttta ttgaaataga
      541 aacattaatt gaaaccgtta tggaaaatgg cagaaaacct ttcttcctaa tccttgatca
      601 actttcagat gctcggaatt tcggtgctat catcagaacg gcagaatgta ctggtgttga
      661 tggtatcata attcaaaaac aaggttctgc tcctgttaat ggtgatacag ttaaaacatc
      721 tgctggcgcg gtgtttaata ttccaatatg taaagtagat catattaaag atgccatctt
      781 tcatttgcaa gctaatggca tcaaaaccgt tgctgctacc gaaaaaacag aacaaaatat
      841 ctatgacatt tctcttaacg aacctttagc cattattatg ggtagcgaag atagaggaat
      901 caatccttcg gtgttgaaaa ttgtagatga aaaagcaaaa ttaccaatgt ttggaacaat
      961 cggttcacta aacgtatctg tagcttgtgg tgccttctta tacgaaactg taagacaaag
     1021 acagtaatcg ctttgtgtta atcattagaa ctctcgttct ttggagtttc aatatattcg
     1081 taaacatatt ttatttcggg agaaaaagtt tctaaaactt ctattatttc ttcttttgga
     1141 ggatttacaa aattgccgtt ttcgtcgaaa cgtttcataa aagcatcgtt ttctgcaaca
     1201 aactctggct tttcccagtc gtattgaatg gatttaacat aatcgggcgt tttataataa
     1261 gcggctaata taaatccggt tagcaatcct gctaaatgtc cttcccaaga aatcttagcg
     1321 tcaacttctg gaaaaacata ccagaccatt ccaccataga ccatgacgac agttaaagag
     1381 agtgccatca atcgatagta tttggtcatc attcctttaa aaaaaataaa ggaaaccaac
     1441 acataaatta atccactagc accaatatga taggagtttc gtccaattat ccaagtgatt
     1501 gttcccgata acaggatgcc atagaccaaa acttttagtg ataaatttcg ataaaaataa
     1561 atcaatgcga gagttaatac aaatagcggt acactattgc tggcaatgtg attaaaatcg
     1621 ccgtgaagaa aaggacttag cactattcct ttcatccccg aaaaggttct cgggaaaatt
     1681 ccatatgggt ttagttttag attaaaagtg tcttctatca aaaaaacagt ccaaattatc
     1741 aacagaaaca atgttggaac aatccatacg gatggtgtaa atttaaagtg gttttgttcg
     1801 tccatgctgt ttgttttact ctcaataatc aaaaatacga ccatttattt tttttatgct
     1861 attttgacag aaatacttat ttttacagta tggaaatccc cttagcagag cgcatcagac
     1921 ctcaaaaact agaagaatac atcagtcaac tacacttagt aggcgacaat ggttcactta
     1981 cgcaacaaat aaaaagagga ataattccat cgatgatttt ttggggttcg cctggaacgg
     2041 ggaaaactac tttagcacag attattgcaa aacaaagtaa tcgtcctttt tatgaattga
     2101 gtgccatcaa tagtggcgtg aaagacattc gtgatgtgat agaaaaagcc aaacaaagtg
     2161 gtggattatt tactgcaaaa aatccaattt tgttcatcga cgagattcat cgttttagca
     2221 aatcgcagca agattcttta ttagccgctg ttgaaaaagg ttggattaca cttattggtg
     2281 ccaccaccga aaatccaagc tttgaagtta ttccggcatt attgtcacgt tgtcaggtgt
     2341 atgttttaaa ttcgttttca aaggatgatt tgattgcatt attacatcga gcaatgaaag
     2401 aagataaaat tatttcgaat aaaaaaatca aattaaagga aacggaagct ttactgcgac
     2461 tttctggcgg agatggacgt aaactattaa atatttttga attggtaatt aacgccagtt
     2521 ctgaaggtca aattatcatt actaataaaa aagtgatgga gttggtacaa caaaacactg
     2581 tattgtacga caaaacaggt gaacaacatt atgatattgt gtcggctttt attaaatcta
     2641 ttcgcggaag tgacccaaat ggtgctgtgt attggttggc tcgaatgatt gaaggcggcg
     2701 aagatgtaaa atttatcgca agacgaatgt taatattgtc tagcgaagat attggcaatg
     2761 ctaatccaac ggctttcatc atggcaaaca atacttttca agcggtttct ataattggtt
     2821 atcctgaaag cagaatactt ttgagccaat gtgctattta tttggcaact tcacccaaaa
     2881 gcaacgccag ttatatggcg ataaacgaag cacaacaagt agttaaacaa accggagatt
     2941 tgtcggttcc tattcattta cggaatgctc caaccaagtt gatgaaggaa ttagggtatg
     3001 gtgaagagta tcaatattct cataattatg ctaataattt ttcggagcaa gagtatttac
     3061 ccgatgaaat tagcactact gctttctata agccaggaaa taatgctaga gaaaatggca
     3121 cgagagagtt tttgaagaat cgctggaaag ataaatacga ttattaagtt cgaggtttga
     3181 aatacgagat acgatgttga agtgtttagc gatttattga aaattaaaat taaatatgaa
     3241 aattatatac aacgaaaaag acaaaaccat tgaaataaat gatggtttga aatcacactt
     3301 tttattgatt aaaatactga tgttgttaat cttaataaat tccgttataa acctaattgg
     3361 tattgcaaaa acaggtttgg gttttatgga aattatttgg ctgtttttag gaacaatctc
     3421 ggtagtggta ctttattctt tatacttcaa agaatcttct ctcgaaaaaa tcccaataac
     3481 gaagattaaa cgattgatgg gaaaaacaag tccgaccaaa aaacaatact attttgagtt
     3541 ggttgatgga aagaaacgag tattgaaaga gctcaaaacc gataaagact ccaaagaatt
     3601 acgaaaacta ttgaccaaat taggaataga aaactaagtt agaatcttaa attaattggc
     3661 tccgaagtta attttccacc ttcgtaacat tcaaaaaacc acttaccgtt tttagtaatt
     3721 acaaccccat tttgatcgcc ttttattccg ataaaaacac ctttttgtga agtagtaaac
     3781 aagcgcatga taacccttgg ctcgttgttt accacctgaa aaccattagc aattggttgt
     3841 gcaaaataaa atatttcaga attttcagaa gataacgaat agggttctac tgattttacc
     3901 tcttgatttt cttctatttt ttctacagct atttgttttt caccattata cttatagttc
     3961 aaggtttcga atgattttgc agcttcacga aaagcttgat tataagcttt gttataatcc
     4021 ttctctctgc tagttccaaa tgctgtttca taaacaattc tatcttgaca gtctttcaaa
     4081 accagtttta ttttagtctg aaacatgcta ttgtctttgg ctaaagaagc cgttaaagtt
     4141 ttacatctat tatttgctat ttctgttggt agttcttgat tgttcataaa cacctcaaat
     4201 ccatattttt ctaataacaa cttagttgat gtatttaaat tgaaactgtt atcctctttc
     4261 aaaaaatcaa actttgttgg aacaataacg aatttgtaat cgtttatatt ttgagaaaaa
     4321 acattaaata ctgtgagtag gaatagtagc gcaatttttt tcatgattat aatatttttt
     4381 ttaattctaa taaatgattt attttttgta cttcatttgt attgtgactg ttagcttgat
     4441 caaatagaat ggctttaata ccaaagttga ttgcgcctcg aatatctgca tcgatgcaat
     4501 caccaatcat taaagcattt tctttggaaa cacttgtcaa agataaggcg aattcaaaga
     4561 tatttggatg tggcttttta acacccgcga tttctgaatt agtaactgtt ttaaaataat
     4621 cattaattcc tgaattgttt aatttctttg tttgaacctc tgcaaaccca ttggttataa
     4681 tatgaagtgt gtatttgggt ttcaaatact ctaagacttc cattgcacca tcaaaaagca
     4741 aattattatc tggtaaaaac tcaatataat catgactaat ttgattgatt tcagcatcag
     4801 aaatagcaaa attaatagca tcaaatgatt gctttaatct ttggtagcga agttcctcat
     4861 gtgtcatttt atcaaactga tataacttcc aacaggcttg atttataggt acatattttt
     4921 ccataaaaac aacagtatca atagttggat attgcttttt gaatatctta tcaaaggctt
     4981 gaatggagtt tttatcaaaa tcccaaagcg tatggtcaag gtcaaaaaaa atgtcggtaa
     5041 tgttattcat cattaaaaaa ttccttcatc ttgaaaactc aaatagccgt tttcggttat
     5101 aataatatga tctaaaactt taatatctaa ttgttcacca gctgctttaa gttttttagt
     5161 tatttgcaaa tcggcatcgc ttgcttgtaa tttcccagat ggatgattat gagataaaat
     5221 tattgatgta gcattttgtt ccaaagcggt tttataaatt agacgaacat cgacaaccgt
     5281 tcccgtaatt ccgccttttg ataattgtgc tttataaatc actttattgg aattattaag
     5341 gtataacacc caaaattctt catgtggcaa ttcgccaatt atgggttgca tgatttcaaa
     5401 taccgcttta cttgaagtta ttttttttaa ttcaacagtt tcttcttccc ttcttcttct
     5461 acccaattcc atagcagcag caattgaaat ggccttcgcc tcaccaattc ctttaaactc
     5521 catcagttgt ttcaacgaca actttcctaa tgcattcaaa ttgttatcta ctgattttaa
     5581 aattcgtttg cttaaatcaa cggcactctc atttctgctt ccagaaccaa taagtacagc
     5641 tattaattcg gcatcactaa gtgcttgttt acctttcagc attagttttt cacgtggctt
     5701 gtcgtcttct gcccagtttt ttatagaaaa atttattggt tccaatttat actaaatgat
     5761 tatttttttc gttattataa aataaagtat aaatgtatga aacccatctc ttttctgcta
     5821 cttctttgct cgtttttaac aacaattacc aaaacttttg aagaaaaact ctcagatgct
     5881 gccatttcta ttattgattc ttcaatagtt tatactccaa actatgttgc tataaaatat
     5941 cctaatggtg atgttccagc aaaaacgggt gtttgcacag atgttgtaat tagagcttac
     6001 cgaaaacttg ggattgattt acaaaaagaa gtgcatgaag acatgaaagc caattttagc
     6061 aaatatccaa attttaaaaa atggggtata aagacttgtg atacgaatat tgaccataga
     6121 agagtaccaa acttagaagt tttttttgaa agaaaaggac aaaaattagc cgttactcaa
     6181 aacgcaaacg attacaaaac tggtgaaatc gtcacatggc tgattgcaaa taaatatcct
     6241 catattggta ttattaccaa taaaaaatca agcgatggaa aacgaccatt aatagttcac
     6301 aatgttggcg gaggacaaat tttagaagat tgtttgttta gctgggagat agttgggcat
     6361 tatagctatg agaagtaaac ttttttattg acatttgttt ttgattatat ttgccataaa
     6421 tatttcaaaa tgaaaaaaat atacatattt atattccttt ttatagtagt taacgttttt
     6481 tctcaacaag ctggagaatt agatttaaac tttggcaatg gtggtttttt tacaaccgaa
     6541 aacaatagcg atattgacaa tgcaaattgc ttatttgttt cagaaagcga tgaaatttat
     6601 gttgctggtg gaaacgataa tttctcaatg ataaaattaa tggctgatgg tacatatgac
     6661 tctgcttttg gcaaccaagg acaagttatc attccttttg acggatttag ggcagaagca
     6721 aaagaaatta tagttcgagc tgatggtaaa attattttag caggaaacgt ttatgtttca
     6781 ggcggagaat ataattttgg tattgtttta ttaaatacag atggctcact agatacttct
     6841 tttaatgaaa ttggtaaact tatttttgat tttgatgaaa acatcaatta tttgtatgct
     6901 gcagctattc aaaatgatgg aaagattgtc gttgctggtc aatgtggaac ttttgccaat
     6961 gcagattttg cagttgctcg aataaatcct gacggcactt ttgataccga atttggagtt
     7021 ggaggaaaac aacgaattaa cattcaaggt gatgatcgcg gtcgatgtgt tgttattcaa
     7081 aatgatggaa aaatagtttt gggaggttat tcatatcctg ttggtgggaa caattgttgg
     7141 tttgccgcgg tacgactaac tactactgga caacttgaca acacgtatag cattgatgga
     7201 aaagcagttg cgaaagtagc aagtagttat gggaatgata ctgtccaaga tatgattatt
     7261 caaccagatg acaaaatact aatggttgca gattcttata ttggaagttc acaagatatt
     7321 ggtatggtaa gatttaccac agatggagag ttagacacta cttttagtgg agatgggaaa
     7381 tttaatacct caatgattgg cacaacagat tatccgcgtg ctatcacatt acaaaccgac
     7441 ggaaagattt tgatttctgg ttctttctat acaactccac aacgaaattt agcattacta
     7501 agattgacag ctaatggaga acttgataca actttcagtc aagacggtaa agcaaattat
     7561 atagtgccag gaaataatgg tgtttctgtt ggggatatga aaacacaatc aactggacaa
     7621 atattaattt gtggaagtat ttttaacaat tacatggttg ctcgcatttt tagtggttca
     7681 gaattgaata cgaatgagta cgaattgaag cgattatttt tttatccaaa tccggcttca
     7741 aaaactattt attttagcga aaatgtaaag tctgtaaatg tatttaccct agaaggtcaa
     7801 aagataatgg aaactattaa ttttccagct caaatggata tttcaactat ttcaaaagga
     7861 atttatattc ttaattttca aacagtttat ggtaaaaaca taaatgaaaa actaattgtt
     7921 gaatgacagc aaatagtaga ttctaaaaaa ttgacttaga caataagtaa tattatatac
     7981 cttattcgac caattgtttc acctcatcaa aattcaatcc gccataattt cctgaactca
     8041 ttaataatag tgctgaatta ttcaaatcat agttaaaaat aaattcttta aatgcagctg
     8101 gattggtata aataattaag tcatcgcgct gaaaagcatt ggctatttga ttataactta
     8161 tttcttccag ttgtttgatt tttacagcat ctggcgaata gaaaactaca gcaacatcgg
     8221 cagcatcaag tgcaccttca tattctttta ggaattcagc attcaaacta ctataggtat
     8281 gtaactctaa acatgccact aattttctgt ctggatattg atttttaacc gctttggctg
     8341 tagcagaaac tttgcttggt gaatgtgcaa aatctttata ggccactttc ccttttcctt
     8401 cggcaatttt ttctaaacgc ttgctcgctc ctttaaaact agcaatggct tcataaaatt
     8461 cagcttcatc aacacccata ttttggcaaa tccatttggc tcctgctaaa ttatttagat
     8521 tgtgtgctcc aaaaacttca atcggcataa aaccttctgg agtttctagc aaagtggttc
     8581 catcttctac tttataattt ggtgtttgat atggtaatct tcggatagta ttcgtggttt
     8641 cttcagctac tttttttact gttttatctt cttcattata gactaaaatg ccacctttga
     8701 taatttgatt gacaaagatt tcaaattgtt ctagatagtt ttcaaaagtt ggaaacacat
     8761 taatatgatc ccaagcaatt cctgaaagta aggcgatatt aggttgatac aaatgaaatt
     8821 tcggtcttct atcaattggt gaggaaaggt attcatcgcc ttcaagtaca ataaaatcgt
     8881 tttcttccgt taaatgtacc atggtatcaa aaccttctaa ttgtgctcca accatataat
     8941 caacttcaat attttgaaaa tgcattacat gcaatatcat ggaagtaatc gtagtttttc
     9001 cgtgtgaacc gccaataact acgcgtgttt tatttttgga ctgttcgtat aaaaactcag
     9061 gataggaata aatttttaat cccaattctt ttgctttcaa taattctgga ttatctgctt
     9121 tggcgtgcat accaagaatt attgcttcaa tatccgaagt tattttctcg ggaaaccaac
     9181 ccatttcaat aggcaataat cctttttttt ctaatcgtga ttttgatggt tcaaaaatag
     9241 catcgtcgct acctgtaact ttgtatcctt tgttgtgtaa tgctattgct agattgtgca
     9301 tggctgctcc accaatggct atgaaatgtg tacgcatttg aaatttcaat tattaaaatc
     9361 ccaaatccca aaactattat gattggaatt tggaatttaa gttttgttat tttttttcga
     9421 attgttcttt tagtttaatt gcttttgctt tatcttttaa tttattttga tataaatcat
     9481 atagtttttg gaatgtagtt ttagaatttc cgatttggta cgctttcaaa taattagttt
     9541 cggctttttt atatcgatca aaatattcgt caatatatcc ttgagaaaga tagccatcta
     9601 cttttgaaag tttcatcaat tcattagaat atttttgagc tttacgttca cttccaccta
     9661 taattcctgg caactctata tataacataa ccaaagccca acgcgaatca atatgttttg
     9721 aatctaattg tgttgctttt agaaaggcat tttcaacatc atcaatcatt cctaatgctt
     9781 tgaatttatt tactgatttg gctttcatac ccattgcgcc tccaaattta taccaataat
     9841 tagcattatt tggattgctt ttttttaatt tttcataata agtgattgca tcctcccatt
     9901 ttttttcaat tccagctata tcacccaaat attcaatagt tttcagatta tttggttctt
     9961 ctttaagaat tttttcgaaa ataggttttg ccaatgaata tttctctgct ttgaaatagt
    10021 tttccgcttt ttgaaagtct atatcatttg aaataaagat caaaattaaa agcccaatat
    10081 tgatgaataa tttcatgttt ttaattaatg tcatttactc aatatcaaac aattaatata
    10141 atatcgatat attttagcaa acaactttat ttgaaaggta aatatacaaa tgttttaaaa
    10201 aattagactg attaaaagag ttattattag catctcaaaa aacaaattgc ctctataaaa
    10261 atgcaattca tcgattatta aagtaatgta tctataaaaa aaatcaatta ggcaaaaatc
    10321 aaactaattt tgtgtcataa ctgcctgatt tctatattca aaaacttatt ttttgattgc
    10381 taaacaaatg tctaacactc ctttttaa
//
LOCUS       DAOOBH010000081        10305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_8133_length_10305_cov_5.113854, whole genome shotgun sequence.
ACCESSION   DAOOBH010000081 DAOOBH010000000
VERSION     DAOOBH010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8133_length_10305_cov_5.113854"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PK218_08495"
     CDS             <1..244
                     /locus_tag="PK218_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98585.1"
                     /translation="TVTKSVSAFPLNDCPKDFPFLFGKEIVYLLASKGKPELEGSDWE
                     TIFANCIGADWKPSNVGLDDVVMGNTACGAKTVKAK"
     gene            440..724
                     /locus_tag="PK218_08500"
     CDS             440..724
                     /locus_tag="PK218_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98586.1"
                     /translation="MSEVAVFEFDTIRYDHELYSWEWNKNNNLTGTNKKTGEHCFTWQ
                     PHGSQFTIVAEVPEKCLIIKIKQPEKLDKDAVLKALGFDKSWVIVSQKVK"
     gene            complement(996..3014)
                     /locus_tag="PK218_08505"
     CDS             complement(996..3014)
                     /locus_tag="PK218_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPW98587.1"
                     /translation="MKKLLLILVAILTFANTNAQIVKPVKWTSKVEKISDTEVNLIMQ
                     ATIEKDWHVYSQYTPDMGPLPAEFKFENAKGNYELIGKVKESPYKKQYNDVFEVDEYY
                     FANNATFTQKVKIINPKLTTIKGSIDFQVCKEVCINDNNVFTFSIPKMVLETKEAVAE
                     APVMEVPTADTTQVETVAVDTTKVKEAVAISPTETPKKEDKKGLLTIFFLAFLGGFAA
                     LLTPCVFPMIPMTVSFFTKQSKTPAQGKRNAILYGLSIIIIYLILGSLVTGIFGAEAL
                     NELSTNVWFNIIFFVLLVVFAVSFLGAFEIMLPNSWANKVDRQADRGGVVGILFMALA
                     LAIVSFSCTGPIVGTLLVESASKGGIAPIVGMLGFSSALALPFMLFAMFPSWLSSLPK
                     SGGWLNTVKVSLGFLELALAFKFLSQADLVLQMHLLEREVFLAIWIAIFGAWALYLFG
                     KITTPHDSPLTHISVGRLSMGLIVLAFTVYMIPGLFGAPLKLISAFPPPQTYSESPRG
                     FFGNSSSTDSKVELPEGAELGEHDIITFHDYEDGFAYAKKVNKSIMLDFTGYACVNCR
                     KMEINVWSDEKVLKVLKNDVVLISLYVDDKRMLPESEQYISKETGKTVKTIGNKWSDF
                     IITRYKTNTQPYYVLTDLNETNLNQPIGYTPNIDEYLAWLKDGVGKFK"
     gene            complement(3014..4321)
                     /gene="tilS"
                     /locus_tag="PK218_08510"
     CDS             complement(3014..4321)
                     /gene="tilS"
                     /locus_tag="PK218_08510"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPW98588.1"
                     /translation="MLDKFQNNISENLPFLPESKLLLAVSGGLDSMVLLHLCQAMKLD
                     ISVAHCNFQLRGEESNNDEKFVTSYCEKNNTPLFVTHFDTEIYAKEQKQSIQVVARNL
                     RYDFFHSVLINNEYDYILTAHHLNDSLETFLINFTRGTGLEGLTGIPQQNEKVVRTLL
                     PFSRVEIEEYAVRNGIQWREDSSNQTEKYQRNKFRHSVVPILQEQNINLLQSFQNTLE
                     YLNQAQSMVEDASHLVYSKVVTEEETIKISLTKLLAFPNYKAYLHQWLAPFGFTAWDD
                     IYNLVSAQSGKQVLSENYILLKDRTYLILFPKKNEEHSDYYWIKKDQKEVKFPLKLSL
                     CNVDYISNEIPNAIFVDEEKLQFPLQIRKWKEGDVLFPFGMNGSKKLSKYFKDEKFSL
                     FDKTNTWLLCSNNQIVWVIGKRQDNRYKITEHTTRILQIKYKP"
     gene            complement(4340..5635)
                     /locus_tag="PK218_08515"
     CDS             complement(4340..5635)
                     /locus_tag="PK218_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HPW98589.1"
                     /translation="MRTHFSKTISNVATFKNQLLLWSQQFREVVFFDSNNYHQQYTSY
                     DCILAVDAFTSIKTDYHNAFDELKQYQQTTKDWLFGYLSYDLKNDVEALQSKNQDGLQ
                     FPDLYFFQPKKLFLIKGNQLEVQYLRLCDDEFVYDFEEIIQSSEIEEEVVMTSAVEVK
                     QRISKENYIQKVTKILDHIHRGDIYEANFCMEFFAENAAIEPLTVYNKLNEISTPPFA
                     TFFKNNTDYLLSASPERYMKKEGFKIISQPIKGTTKRFEEVTLDEKSKVDLENDPKER
                     SENIMIVDLVRNDLSHTASKGSVTVEELCQIYSFKQVHQMISTVVSKVDNSIAPIDIL
                     KTTFPMGSMTGAPKISAMNIIEALEETKRGLYSGSVGYFTPQNDFDFNVVIRSILYNA
                     KENYISFSVGSAITSLSQPKKEYEECLLKAKAMFSVLSE"
     gene            5812..6702
                     /locus_tag="PK218_08520"
     CDS             5812..6702
                     /locus_tag="PK218_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HPW98590.1"
                     /translation="MKNYSKLGLVLLLIGLAFACKQADHSMETESTTENKTSADMISS
                     NAAVEPKNSNRKFVRTADIKFKVKNVAQSTYAIENSIAKHGGFVTLTDLKSNINERNS
                     TKISQDSILETTKYTVDNSITFRVPNTAMDTVLKSIVNQIEFLDNRLIKADDVSLQML
                     SNKLTQKRLQKSQKRLEKGIDFKGKKIDQIVNAEDRVLDKESDADASQLNTLSLEDQV
                     NFSTVTLYLYQRETTRQELIKNETNTDAFRPSIGLQIVDSLKTGWYVLEVIIAFIVQM
                     WSILLLVFFGFIIYRNYLKK"
     gene            6796..6918
                     /locus_tag="PK218_08525"
     CDS             6796..6918
                     /locus_tag="PK218_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98591.1"
                     /translation="MKIATIIVRVLLGLVFIFASMSFFLKLAPEPVTTGDFKAF"
     gene            complement(7202..7459)
                     /locus_tag="PK218_08530"
     CDS             complement(7202..7459)
                     /locus_tag="PK218_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98592.1"
                     /translation="MKLPIIKQLTQFIEENDQDYIVETIEVLENLCEISSLKDEELDV
                     IAELISNMYGALEVHKMVKDGTDKKIALNDFMKRVLGSIDK"
     gene            complement(7555..7863)
                     /locus_tag="PK218_08535"
     CDS             complement(7555..7863)
                     /locus_tag="PK218_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPW98593.1"
                     /translation="MYIEIEQDNLAEVVANNEKVVVQFSATWCGNCRIMKPKFKLLSA
                     QNESISFVMVDAEKFPESRKMAKVDNLPTFATFKNGVLVNQTQTNKVDVLTDLVSEIL
                     "
     gene            complement(7942..8577)
                     /locus_tag="PK218_08540"
     CDS             complement(7942..8577)
                     /locus_tag="PK218_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPW98594.1"
                     /translation="MALVGKKFPNITVDAISFMGDNLRINVLEEAIENKKKVVLFWYP
                     KDFTFVCPTELHAFQAALEEFEKRNTMVIGASCDTNEVHFAWLNTAKDNGGIEGVTYP
                     IIADTTRNLSAALDILDAEWVYDEVLNDEILEGSNVTFRATYLIDEDGKIFHESVNDM
                     PLGRNVNEYLRLIDAYTHVQTKGEVCPANWEEGKDAMSADRNSTAAYLASH"
     gene            8789..>10305
                     /locus_tag="PK218_08545"
     CDS             8789..>10305
                     /locus_tag="PK218_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPW98595.1"
                     /translation="MGFSNLFRKKSVQDILDQVAKNESDGHATLGKHLTAKDLAAFGI
                     AAIIGAGIFSTIGKASADGGPAVIFLFLFTALACGFAAFAYAEFASMVPVSGSAYTYS
                     YVAFGELIAWIIGWALIMEYAIGNITVAISWSDYFTGLLESGGIYMPQWAQMDYLTAS
                     NGFNDANAFMQGGKSFDKLPEYLQQAHTAWTTAPTIGNFHFVADFPALLIIILITWLV
                     YRGMKESRNASNLMVVVKLCIILLVIAVGAFYVDTANWHPFAPNGVTGVLKGVSAVFF
                     AYIGFDAISTTAEECKNPQRDLPKGMMWAIIICTILYIVIALVLTGMVNYADLNVGDP
                     LAFVFDKLNLKWMSGIIAVSAVIAMASVLLVFQMGQPRIWMSMSRDGLLPKKFSKVHP
                     KYKTPSYATIVTGFVVAVPALFLNLTMVTDLCSIGTLFAFVLVCAGVLVLQNKKDIPR
                     GKFKTPYFNAKYVFPLLIIFGLVFAFTAKKEQTMAFFTNEKQINDPTTIVTTLTK"
BASE COUNT         3265 a         1681 c         1765 g         3594 t
ORIGIN      
        1 aacagttact aaatctgttt ccgcatttcc tcttaatgat tgtccaaaag attttccatt
       61 tctgtttgga aaagaaatag tttatttact tgcttcaaaa ggaaaacctg aattagaagg
      121 ttcagattgg gaaactattt ttgcaaattg tattggagca gattggaaac catcaaatgt
      181 tggactagat gatgttgtaa tgggtaatac tgcttgtggc gcaaaaactg ttaaagctaa
      241 ataaccatca aaacagaaaa aaattaggct gatttctgga agaaattctc caaattattc
      301 atttggagag cgtagcgatg aaaaagctga cccaaaatta attggagaac ttgtgctaga
      361 aatttggaat gagagagttt cttcaattcg tgaaaagtat aagcatttaa gaactgttgt
      421 gcttttaaaa tcaaatgata tgtcagaagt tgccgttttt gaatttgaca ctatccgata
      481 tgatcatgag ctttatagtt gggaatggaa caaaaacaat aatctaacag gaacaaataa
      541 aaagactgga gaacattgtt ttacttggca acctcacggt tcacaattta ccattgttgc
      601 agaagttcct gaaaaatgct tgattataaa aattaagcaa cctgaaaaac ttgataaaga
      661 tgctgttctt aaagctttag gatttgacaa atcttgggtt attgtttctc aaaaagtaaa
      721 ataataacta ttcattatat gattgtaaaa ttagttctta tttctcgtgt tttgctgagt
      781 tgggatgaca aatggtttgt ttagttttta agttttcaaa aaaagcccaa caaaaaacca
      841 aaactgttgg gcaaactaat taagtataaa ccaaaacaaa ttatatttta ttttctaaag
      901 actcatatcg ttttatgatg gtattcttgt ttagttgcag tggatgattt taaggcataa
      961 aaaacgctgc cgaagcaacg ttttatatgt atttgttatt taaacttccc tactccgtct
     1021 tttaaccaag cgaggtattc gtcgatgttg ggtgtatagc caataggttg gtttaaatta
     1081 gtttcattca agtcggtcaa cacataatag ggttgtgtgt tggttttata tctggtaatg
     1141 ataaaatcac tccatttgtt accgatggtt ttcaccgttt tgccggtttc tttcgaaata
     1201 tactgctcgc tttcaggaag catgcgtttg tcatctacat ataatgaaat caaaacgacg
     1261 tcgttcttta atactttcaa aactttttca tctgaccaaa cgttgatttc catttttcgg
     1321 caattaacac aagcgtatcc tgtaaaatcg agcatgattg atttgttgac tttcttggca
     1381 taggcaaatc cgtcttcgta atcgtgaaag gtgatgatgt cgtgctcacc aagctctgct
     1441 ccttctggca attctacttt tgaatcagtt gaagatgaat ttccaaagaa acctcttggg
     1501 ctttcgctat aggtttgtgg tggtggaaaa gcactgatta gttttaacgg cgcaccaaac
     1561 aatcctggaa tcatatagac cgtaaaggct aatacaatta accccataga caatcgacca
     1621 accgaaatgt gggttaatgg gctgtcgtgt ggcgtggtga tttttccaaa taaatacaat
     1681 gcccaagcac caaaaatggc tatccaaatg gctaaaaata cttctctttc taataggtgc
     1741 atttgcaata ctaaatcagc ttgagataag aatttaaaag ctaaggctaa ttctaaaaat
     1801 cctaacgaca ctttcacggt atttaaccac ccacccgatt ttggcaaaga acttaaccaa
     1861 cttgggaaca tggcgaataa cataaacggc aatgctaatg ctgaagaaaa accaagcatt
     1921 cctactatcg gtgctatacc tcctttggaa gctgactcaa ctaaaagtgt tccaacgatt
     1981 ggtcctgtac acgaaaagga aacgatggct aaagctaatg ccatgaataa aatacctacg
     2041 actccacctc tatcggcttg tctatcgact ttatttgccc aagaattagg caacatgatt
     2101 tcgaaggcac ccaaaaaaga aactgcgaaa actactaata aaacaaagaa aattatattg
     2161 aaccaaacgt tggttgagag ttcgttgagc gcttcggcac caaatattcc ggtgactagc
     2221 gaaccgagaa ttaaataaat aatgatgatg gataatccgt ataaaatggc gtttcgtttt
     2281 ccttgcgctg gtgttttact ttgttttgta aagaagctca ctgtcatggg tatcattggg
     2341 aaaacacatg gcgttagtag ggctgcaaaa ccaccaagaa atgccaaaaa gaagatggtt
     2401 aacaatcctt ttttatcttc tttttttgga gtttcggtag gtgatatagc tacggcttct
     2461 ttaacctttg ttgtgtcaac agcgacagtt tccacttgtg ttgtgtcggc tgtaggcact
     2521 tccattactg gtgcttcggc aacggcttct tttgtttcca aaaccatttt aggaattgaa
     2581 aaggtaaaca cattattatc gttgatacat acttctttgc acacttgaaa atcaatagaa
     2641 cctttgattg tagtcagttt aggattgatg atttttactt tttgggtgaa ggttgcgttg
     2701 ttggcaaaat agtattcatc tacttcaaaa acgtcattgt attgtttctt gtatgggctt
     2761 tctttgactt tccctattag ttcgtaattg cctttagcat tctcaaattt gaattcggct
     2821 ggcaacggac ccatatctgg cgtgtattgc gaataaacgt gccaatcttt ttcgatggtg
     2881 gcttgcataa taagattgac ttctgtgtcg cttatttttt caaccttact ggtccatttt
     2941 acgggcttga caatttgagc atttgtgtta gcaaaagtca aaattgctac taaaattaaa
     3001 agtaattttt tcattagggt ttgtacttta tttgtaaaat tcgtgttgtg tgttctgtta
     3061 ttttatatct attgtcttga cgttttccaa taacccaaac gatttgattg ttggaacaaa
     3121 gtagccaagt gtttgttttg tcaaaaagag aaaacttttc gtctttgaaa tatttgctca
     3181 atttcttgga accattcatc ccaaaaggaa acaaaacatc accttctttc cactttctaa
     3241 tttgcaatgg aaactgcaat ttctcttcgt ccacaaatat agcatttgga atttcatttg
     3301 aaatgtaatc tacgttacaa agcgacaatt ttaagggaaa tttaacttct ttttggtctt
     3361 tctttatcca ataataatct gaatgttctt catttttttt tggaaacaaa ataagatatg
     3421 ttctgtcttt tagtaaaatg taattttcgg aaagcacttg ttttcccgat tgtgccgaaa
     3481 ctaaattgta gatatcatcc caagcggtga acccaaatgg tgctaaccat tgatgcaaat
     3541 aggctttgta attgggaaac gccaatagtt ttgttagact aattttgatg gtttcttctt
     3601 cggtaactac tttagaatac accaaatgac tagcatcttc taccatagat tgtgcttggt
     3661 ttaggtattc taacgtgttt tgaaacgatt gtagcaagtt gatgttttgt tcttgaagaa
     3721 ttggtaccac agaatggcga aatttgtttc gttggtattt ctccgtttga ttgctgctgt
     3781 cctctcgcca ctgaattcca tttctaacag catattcttc gatttcaact ctggaaaatg
     3841 gtagcaaggt gcgcactact ttttcattct gctgtggaat tcctgtaagt ccttccaaac
     3901 ctgtaccgcg cgtgaaattg attagaaatg tttccaaact gtcgttcaaa tgatgtgctg
     3961 ttagaatgta atcgtactca ttatttatca aaacagaatg aaaaaaatcg tagcgtaagt
     4021 tacgagccac cacttgaatg gattgttttt gttctttggc ataaatctca gtatcaaaat
     4081 gagttacaaa taatggggtg ttgttttttt cgcaatagga tgtaacaaat ttttcatcgt
     4141 tgttactttc ttctccacgt aattggaagt tgcaatgtgc cacggagata tcaagtttca
     4201 ttgcttggca taaatgcaat aacaccatgc tatctaatcc accactaacc gccaatagga
     4261 gtttgctttc aggcagaaaa ggtaaatttt cagagatatt attttgaaac ttatccagca
     4321 tcgtcaaaaa taagaatttt tattcactca aaacagaaaa cattgctttg gcttttaaca
     4381 aacattcttc atattctttt ttgggttgag ataatgatgt aatggcactc ccgactgaaa
     4441 aggaaatata gttttctttg gcattataca aaatacttcg gatgacaaca ttaaaatcaa
     4501 agtcgttttg gggtgtaaaa tagccaacac taccgctgta taaaccgcgt tttgtttctt
     4561 ctagtgcttc gatgatattc atggctgaaa tttttggcgc gcctgtcatg cttcccattg
     4621 ggaaagtagt ttttagtata tcgataggag caatactgtt atcgactttg gaaacaacag
     4681 tggaaatcat ttgatgcact tgtttgaagg aataaatttg gcaaagttct tcaacagtta
     4741 ctgaaccttt tgaggctgta tgtgataaat cattgcgaac taaatcgaca atcatgatgt
     4801 tttcagaacg ctcttttggg tcgttttcta aatctacttt tgatttttcg tctaaagtta
     4861 cttcttcaaa acgtttggtg gttcctttta tgggttgcga aattattttg aagccttctt
     4921 tcttcatata acgctctggt gatgccgaaa gcaaataatc ggtgttgttt ttaaagaaag
     4981 tggcaaacgg cggcgttgaa atttcgttta gtttattata aaccgtcaat ggttcgatgg
     5041 cagcgttttc tgcaaaaaac tccatacaaa aattagcttc atatatatct ccacgatgga
     5101 tgtggtctaa tattttggtg actttctgaa tataattttc ttttgaaatg cgttgtttca
     5161 cttcaactgc acttgtcatg acaacttctt cttcaatctc agagctttga ataatttctt
     5221 cgaaatcgta cacaaattcg tcatcacata atcgtaaata ctgcacttca agttgattgc
     5281 ctttaatcaa aaaaagtttt ttgggttgaa agaaatacaa atcgggaaac tgcaaaccat
     5341 cttgattctt tgattgcaac gcttccacat cattttttaa atcataggac aagtaaccaa
     5401 acaaccaatc tttagtggtt tgctggtact gttttaattc atcaaaagca ttgtgataat
     5461 ctgttttaat tgatgtgaat gcatcaactg caagtatgca atcataggaa gtatattgtt
     5521 gatggtaatt gttgctgtcg aaaaaaacaa cttctctaaa ctgctgtgac cacagtaata
     5581 gctgattttt gaacgttgca acattcgaaa tagttttaga aaaatgagtt ctcaaagtga
     5641 tttcttttga cctcaaaatt acgataaaaa aatagtctta agcaaaaatt aagtttggca
     5701 cgctttttag tagtaattct ttttaacgaa ccttacctca ttttgcatat tgataacact
     5761 atactttttt aaacattttt gaaatactat taacaattaa aaaaaagtaa aatgaaaaac
     5821 tattcaaaac taggattggt attgcttcta atcggtttgg cttttgcatg caaacaagct
     5881 gaccactcga tggaaacaga aagtacaaca gaaaacaaga catcagcaga tatgatttct
     5941 tccaatgctg ctgtggaacc caaaaattct aatcgaaaat ttgttcgaac ggcagatatt
     6001 aaatttaaag taaagaacgt tgcacaatct acttatgcta ttgaaaatag cattgcaaaa
     6061 catggtggat ttgttacgtt aactgattta aaaagcaaca tcaacgaaag aaatagcaca
     6121 aaaatcagtc aagattctat acttgaaact acaaaatata cggttgataa ttcgattaca
     6181 tttagagttc caaacacggc aatggatact gttttaaaat cgatagtaaa ccaaattgaa
     6241 tttttagaca acagattaat taaggctgat gatgtttcat tgcaaatgtt atccaataaa
     6301 ctcactcaaa aaagattgca aaaaagtcaa aaacgattgg aaaaaggaat tgacttcaaa
     6361 ggaaaaaaaa ttgaccaaat tgtgaatgct gaagacaggg ttttagataa agaatctgat
     6421 gctgatgctt cgcaattaaa cacgttgagt ctggaagatc aggttaactt tagtacagtg
     6481 acgttgtatt tgtaccaacg agaaactaca aggcaagaac ttataaaaaa tgaaacaaat
     6541 actgatgctt ttcgtccaag tattggcttg caaatagttg atagtttaaa aacaggttgg
     6601 tatgttttgg aagtaatcat tgcatttatt gtccaaatgt ggtctattct tttattagtt
     6661 ttttttgggt ttattatcta tcgaaactat ttgaagaaat aataaaacac tgacattaaa
     6721 caaagtaaac accaatatcg tatagagttg gtgttttttt gtttaacttt gatacaatca
     6781 ataaaccaca aaaccatgaa aattgcaact attattgtta gagtattgtt aggactggta
     6841 tttatttttg cttcaatgag tttctttttg aagttagcac cagaacctgt aacgaccgga
     6901 gattttaaag ctttctaaat tgggcttgta gcttcatctt acttaatccc attagcaaaa
     6961 ggaattgaat tattatgtgg attatcgttt ttagtaggta aatatgtaac cttagccaat
     7021 attgttattc ttcctgtaac attaaacatt ttgatgatta attattttat gtcgcctgaa
     7081 actttgccta ttgccattgc tttgttttta gggaatttgt ttctaattta tagttataga
     7141 gaaaattaca aaactgtttt taaaccataa aaaaacgtcc tgaaatttca ggacgttaat
     7201 tttatttatc tattgaaccc agtacccgct tcataaaatc attcaaggct atttttttat
     7261 cagtgccgtc tttgaccatt ttatgtactt ctaacgcacc atacatatta gaaattaatt
     7321 ctgcaattac atctaattct tcatctttca aggaagaaat ttcacataaa ttttctaaaa
     7381 cctcaatagt ttcgactata taatcttgat cgttttcctc aataaattga gttaattgtt
     7441 ttattatagg taatttcatg aaaattttgt tttaaagttt aatggtttat aagtttaatg
     7501 tattaatgga aaaagtaata ttcattaatg caatttacac gaaattaaaa ttcattaaag
     7561 tatttcactt actaaatcag ttaaaacatc aactttattg gtttgtgttt gattaaccaa
     7621 aactccattt ttaaaggtgg caaaggttgg taaattatct acttttgcca tttttctaga
     7681 ttcaggaaac ttttctgcat caaccatcac aaaagaaata ctttcatttt gagcagataa
     7741 cagtttaaat tttggtttca taatacgaca attaccacac catgttgctg aaaattgtac
     7801 tacaactttt tcgttatttg ctacaacttc tgctaaatta tcttgttcta tttctatata
     7861 catacttttt ttttcaaatt tgtaaaaagc ttcaagtttt aagtataact aaataaactt
     7921 aaaacttgaa acactttttt attaatgaga agctaaataa gcagcagtac tgtttctatc
     7981 ggcactcatc gcatcttttc cttcttccca gttagcagga caaacttctc cttttgtttg
     8041 aacgtgtgta taggcatcta ttaatcgtaa atattcattt acatttctac ccaatggcat
     8101 atcattaaca ctttcatgga agatttttcc gtcttcatca attaaataag tagccctaaa
     8161 agttacattt gatccttcaa gtatttcatc gtttagaact tcatcataaa cccattctgc
     8221 atctagaata tctaatgccg cagataaatt tcttgtggta tctgcaatta taggataggt
     8281 tacgccttca attccaccat tgtctttggc tgtatttaac caagcaaagt gaacttcatt
     8341 tgtatcgcat gaagcaccaa taaccattgt atttcttttt tcaaattctt ctaaagctgc
     8401 ttgaaaagcg tgtaattctg ttggacatac aaaagtaaaa tcttttggat accaaaataa
     8461 aactactttt ttcttgtttt caattgcctc ttctagcaca ttaattctta aattgtcacc
     8521 cataaaagag attgcatcaa ctgtaatgtt tgggaatttt tttccaacta aagccatatt
     8581 gtattatttt aaattagtat tattttctat tgcaaagata ctaccaaatg caactcataa
     8641 caataagttt tgattatatt taattataaa acaatagaca tatactattc gtattgaaaa
     8701 aacaatataa ctatatcctg aaaaatttaa aaccaagcag atttaattat atttgttttt
     8761 tttaaaccat aaataaccaa agccatatat gggattttca aatcttttcc gaaaaaaatc
     8821 agttcaagat attttagacc aagttgccaa aaacgaaagt gatggtcacg ctactttagg
     8881 aaaacattta acggcaaagg atttagcagc ttttggaatt gcagctatta ttggtgctgg
     8941 aatattcagt actattggaa aagccagtgc agacggtggt ccggcagtta tttttttatt
     9001 cctatttacg gctttggctt gtggatttgc agcatttgct tatgctgaat tcgcttcaat
     9061 ggttcccgtt tctggaagtg cttataccta ttcctatgta gcttttggtg aattaattgc
     9121 ttggattatt ggttgggctt tgattatgga atatgctatt ggaaacataa ccgttgctat
     9181 ttcgtggagc gattatttta caggattgct agaaagtggt ggtatttata tgcctcaatg
     9241 ggcacaaatg gattatttaa cagcttctaa tggttttaat gatgctaatg cctttatgca
     9301 aggcggaaaa tcttttgata aattacctga atatttacaa caagcgcaca ccgcttggac
     9361 aactgcacca acaattggaa attttcattt cgtggctgat tttcctgctc tattgataat
     9421 tatattaatc acttggttgg tttatcgtgg gatgaaagaa tcacgaaatg ctagtaattt
     9481 gatggttgta gtaaagctct gcattattct tttagtaatt gccgtaggtg ctttttatgt
     9541 tgataccgct aactggcatc cttttgctcc caatggagtt acaggagttt taaaaggtgt
     9601 ttcggctgtt ttttttgctt atattggttt tgatgcgatt tcaactactg ccgaagaatg
     9661 taaaaatcct caaagagatt tacccaaagg aatgatgtgg gcaattatta tttgtacaat
     9721 attatacatt gtgattgctt tggtattgac aggaatggta aattatgcag atttaaatgt
     9781 tggtgaccca ttagcatttg tatttgataa attaaactta aaatggatgt cgggcatcat
     9841 agctgttagt gctgttattg ctatggcaag tgttttatta gtctttcaaa tgggtcaacc
     9901 acgaatatgg atgagtatga gccgtgacgg attattgcca aagaaattct caaaagtgca
     9961 tccaaaatac aaaacccctt cgtatgctac tattgtaacg ggatttgttg tggctgttcc
    10021 agccttattt ttaaacctaa cgatggttac tgatttgtgt agtattggaa ccctatttgc
    10081 ttttgtgctg gtttgtgctg gtgtattggt gttacaaaac aaaaaagaca ttcctagagg
    10141 aaaattcaaa acaccttatt ttaatgcaaa gtatgttttt cctttgctta ttatttttgg
    10201 gttggtgttt gcttttacag caaaaaaaga gcaaactatg gcttttttta ctaatgaaaa
    10261 acagataaac gatccaacaa ctattgtaac aactttaaca aaaga
//
LOCUS       DAOOBH010000082        10144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_8287_length_10144_cov_4.938150, whole genome shotgun sequence.
ACCESSION   DAOOBH010000082 DAOOBH010000000
VERSION     DAOOBH010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8287_length_10144_cov_4.938150"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..710
                     /locus_tag="PK218_08550"
     CDS             345..710
                     /locus_tag="PK218_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98596.1"
                     /translation="MKSTNFRYNINYKASDDDTKIIVLSQLPFFKGSETEIEIPLNDI
                     DNVIESLKFAKDEIKNIDLDEIREVSKVILDASIVNTLVSLFLSGIPTSVLAKQYDLD
                     ENVVKENLEEKGIILFEEI"
     gene            788..2308
                     /locus_tag="PK218_08555"
     CDS             788..2308
                     /locus_tag="PK218_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HPW98597.1"
                     /translation="MIKKIYLFFFCGFGLISNAQNGYDISVTIKNCPDTLAYLTFYQY
                     DKTLIKDTCTTIKNGKIIFKGKSKLEKGIYSIVSQQKSIYFDFFVDDATQKLVVETEY
                     GPELVKKLQSPTSQKENDFFDYIKYINKEGADFQIWKQNLNAQTKKDSLKIFDKQKEF
                     EEKIQAVEESFVIKNKGSYIADVINLKMERTLKNPPKASNGRTDSIATFNYYKKHFWD
                     DVNFKDEGIYRNPFFHLKIKKYLDQVVYPHPDTLIVAVDKIIEQTTPGSLLNKLLIGN
                     LTYTYESSKKMGFDKVFVHLSDKYFKTGKAVGIYNDDSVIQKIIKRADKLKPITVGKI
                     APDLSMIDVKNGPRMKQLGFEKANTSEEVTKLFYDNTNELNKIFVKLHDVKAEYTILI
                     FWDVDCGHCQKEIPVLLDVYHELLKEKKDVKVYSVYTLFDSEKYQKYIDEHKLDFINL
                     YDASHLNNVIEIYDVYSTPVIYILDKDKRIKAKRIGVEQIKPIITALENEAKSSKS"
     gene            complement(2311..3471)
                     /locus_tag="PK218_08560"
     CDS             complement(2311..3471)
                     /locus_tag="PK218_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPW98598.1"
                     /translation="MENFKMLAKTFFGFEEILAKELQILGAQNVEIGTRAVSFKGDKG
                     FMYKANLSLRTALKILKPIYQFRAFNDLSLYKGIQGIDWSKYLNENQTFVIDTTIHSD
                     HFNHSQFVSQKAKDAIVDQFRVKKGVRPSVDKDFPDLKINIHIDKDQCTVSLDSSGES
                     LHQRGYRTATNIAPINEVLAAGMLMLSGWDGSSDFMDPMCGSGTLLAEAAMIACNIPA
                     NINRKEFAFEKWNDWDNDLFDQIIDALLKRTKEFRYTITGYDKAPSAVQKAKDNILNA
                     NLEDYVTIHQANFFESKKENVGPLHMVFNPPYGERLDIDLERFYRELGDTLKNNYSNT
                     NAWFITANLDALKFVGLRPSRKIKLFNGSLEARLVKYEMYEGSKRAKFQTEG"
     gene            3601..4344
                     /locus_tag="PK218_08565"
     CDS             3601..4344
                     /locus_tag="PK218_08565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW98599.1"
                     /translation="MSEINKTTTKNWFVSWFDSPYYHILYKDRNYREAQLFMDTLTHY
                     LNLPEKAKVLDLACGKGRHSIYLNQLGFDVIGADLSENSIEEANKNANDNLHFLVHDM
                     RESYEEKFDAIFNLFTSFGYFENEQDDLKTLVAIKESLAEYGFAVIDFMNSYQVIENL
                     IPEETKTVDGIDFHIKRYVHDGFIIKEIDFEDKGEIFHFVEKVRGYTLEDFKRLMEEA
                     GIYLLDVFGDYKLKKFHKNTSERLIMIFK"
     gene            4452..5120
                     /locus_tag="PK218_08570"
     CDS             4452..5120
                     /locus_tag="PK218_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPW98600.1"
                     /translation="MNYILPLLSVLIGYVFAISIKPKDKKNLKLLLAFSGSFLLSLTV
                     LQLLPEVYKNPTKRIGIFIMAGILFQIILEFFSKGAEHGHVHGHDKIHQMPWLLFVSL
                     CLHALLEGFPVGHHHDLAIGIAIHHLPIAIILTTFFLNAGLNKTALLLFMITFALMTP
                     IGTFLSDTFPILNDYYREITAVVIGILFHISSTIIFESSEGHKFNIAKVSMIILGILL
                     ASFL"
     gene            5156..5968
                     /gene="murQ"
                     /locus_tag="PK218_08575"
     CDS             5156..5968
                     /gene="murQ"
                     /locus_tag="PK218_08575"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HPW98601.1"
                     /translation="MNFTKTTEQSSHYEHLEQMSVKELLTNINNEDKTVPLAVEKALP
                     QIEALVAKTVEKLKLGGRLFYIGAGTSGRLGIVDASECPPTFGVPFELVNGIIAGGDK
                     AIRKAVENAEDNRKQAWIDLQNETISANDVVIGIAASGTTPYVIAGLEKCNENNIVTG
                     CITCNEGSPLALTAKYPIVVVVGPEFVTGSSRMKAGTAQKLVLNMISTSVMIQLGKVK
                     GNKMVDMQLSNDKLVDRGVKMIMSEIPVDYETGSKLLKVHGSVRKAVDNFKK"
     gene            5973..6434
                     /locus_tag="PK218_08580"
     CDS             5973..6434
                     /locus_tag="PK218_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98602.1"
                     /translation="MKKLVLILVLLFANTVFSQQIKLEKGKYFVDGQQISTRETRQLL
                     LTDYKATRLFQKAKSKEGIGGLLLGAGIAMTVTDLAIGLFSDVKYPTAITYVGVATMA
                     ISVPILSGRRKMIKEAIDSYNQTAKQLGSNDDSFKYQIVANQNGIGIQLKF"
     gene            6437..6664
                     /locus_tag="PK218_08585"
     CDS             6437..6664
                     /locus_tag="PK218_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6095 family protein"
                     /protein_id="HPW98603.1"
                     /translation="MPTNRNLLNKGIKYLVYSIPLIFIGPTVIYNAFMNKHTNWHYLV
                     LAIGCLMCLGAMFFIFLGIKTITDSLFNDDK"
     gene            6654..7226
                     /locus_tag="PK218_08590"
     CDS             6654..7226
                     /locus_tag="PK218_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HPW98604.1"
                     /translation="MINNNYLESIKKQMLYYKTLAEKAIDQLEEAQLFVSNNDDTNSI
                     AVIVNHMSGNMLSRWTDFLTTDGEKEWRQRDAEFENSINTKETLLATWNKGWDCFFNT
                     INALEADDLEKIIYIRNEGQTVVEAINRQLAHYPYHVGQIVFYAKMLKQDSWSSLSIP
                     KNNSQSYNAEKFAKDKTIKHFTDDELTKLQ"
     gene            7237..7623
                     /locus_tag="PK218_08595"
     CDS             7237..7623
                     /locus_tag="PK218_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV"
                     /protein_id="HPW98605.1"
                     /translation="MKSFLTNYLFPTVLLLLLSSCYQQERKCADFKTGTFQFKQEIDG
                     KEHTTTFIRTNDMQIETFNGKTDTATVRWVNDCEFILEKLHPRNMQEKKAISMKILYT
                     KDNTYTFEYAFVGDEKKMKGLVTKLE"
     gene            7657..8514
                     /locus_tag="PK218_08600"
     CDS             7657..8514
                     /locus_tag="PK218_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPW98606.1"
                     /translation="MEILFTPNAIIALLTLTFLEIILGIDNIVFLSIVSGKLKEEDQP
                     KARRIGLLLAMAFRIILLFGITWVLSLQDTLLSIDWSFFSAHITGQSLIIFGGGLFLL
                     YKSVSEIHHKLEGEEESEKGKSSNTISQAIIQIALLNIVFSFDSILTAVGMVSMKEPA
                     EGGFGYEGALFIMILSVVISIIIMMVFAGPVSKFVNEHPTIQILGLSFLILIGVMLLA
                     EGSHLAHFRFGNDIEVNSIPKGYLYFAIFFSLFVEFLNLKMKKSKNKVKLHNSTIVDE
                     KLKDGDATL"
     gene            8527..8745
                     /locus_tag="PK218_08605"
     CDS             8527..8745
                     /locus_tag="PK218_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98607.1"
                     /translation="MDNKTLFTHPILRTVLIFGGLWLITTVLVIAVITNMFVEPIIQP
                     KYFVLYMLLFASTSTMVTLIKNYIKTRK"
     gene            complement(8818..>10144)
                     /gene="eptA"
                     /locus_tag="PK218_08610"
     CDS             complement(8818..>10144)
                     /gene="eptA"
                     /locus_tag="PK218_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase EptA"
                     /protein_id="HPW98608.1"
                     /translation="SLSRRVGKFLLVLFFCINAIAVYFVNTYGVIIDESMIGNVLNTK
                     YGEASSFFSLKLIAYLVLFGVIPSIYIFKVKIIKDTWKKFLITISLTLIFILALAFAN
                     ASNWLWIDKNSKTLGGLAMPWSYTVNISLFYAHEFKKNQKEILLPNAIVKNNEKSVVV
                     LVIGESARSQNFSLYGYDKNTNPLLSKITNVFHFNAISSATYTTAGVKAILDYKNTDE
                     LYEILPNYLYRNNVEVIWRTSNWGEPPVHIKNYQKEDVLRPNCKGEGCGYDEVLLTGL
                     KEQIRASTKNKILIVLHTSTSHGPTYSKKYPPQFETFKPVCNSVELGNCSKKELINAY
                     DNTIVYTDYILSKVIEDLKELKDYKSTMLFVSDHGESLGEKNLYMHGIPMSIAPKEQY
                     EIPFIVWISDNSRQLKANKELTQYHVFHSVLNFLSIESPIYDEEMNIFK"
BASE COUNT         3552 a         1602 c         1660 g         3330 t
ORIGIN      
        1 aaaaaactta ttttacactt ttttattttt tatacttaaa aaagtaattt aaaaatgtta
       61 aattacacaa acgtataggt aaaatatgag tttatttcac ttccaaaagt tactgcaaaa
      121 gtgagttaat taaatatgca tttcaacaga cttatcacac tattcatcga aaaaccaaca
      181 aattactact taaattttgc tcgcacaaaa aagttattat caattttact ttacaattgt
      241 taattaattt tggttcataa cagtgttaaa atttgaatat ttaaagactt aattccttac
      301 ttttgaatac ccaaatttat tattaactta aacactgcaa cattatgaaa tctacaaact
      361 tcaggtacaa catcaattat aaagcaagtg atgatgatac aaaaattatt gttttatctc
      421 aattaccatt ttttaaaggt tcagaaaccg agatagaaat tccacttaat gacatagata
      481 atgtaattga gtcgttgaaa tttgctaaag atgaaataaa aaacattgat ttagatgaaa
      541 taagagaagt gagtaaagta atattagatg ccagtattgt caatacatta gtgtctttat
      601 tcctttcggg cattccaact tctgttttgg caaaacaata cgatcttgac gaaaatgttg
      661 ttaaagaaaa tcttgaagaa aaaggaatta ttttgtttga agaaatataa ttaatcaaaa
      721 attaagtttt tttaacgttc gaaatgcgct atttttgtgt gtaaagcata aacatgtaca
      781 ttagataatg ataaaaaaaa tatacttatt ctttttttgt ggttttggtt taatatcaaa
      841 tgctcaaaat ggttacgata tatcagtaac tattaaaaac tgtccagata ccttagccta
      901 tttaacgttc tatcaatatg ataaaacatt aataaaagac acctgtacta ctattaaaaa
      961 tggtaaaatc atctttaaag gcaaatcaaa actagagaaa ggaatctatt ctattgttag
     1021 ccaacaaaaa tctatttatt ttgatttttt tgttgatgat gcgacgcaaa aacttgtggt
     1081 tgaaacagaa tatggtccag aattagttaa aaaattacag tcgcctactt ctcaaaaaga
     1141 aaatgatttc ttcgattaca ttaaatacat caacaaagaa ggtgccgatt ttcaaatttg
     1201 gaaacagaat ttgaatgcac aaaccaagaa agattctttg aagatttttg ataaacaaaa
     1261 agagtttgaa gaaaaaattc aagctgttga agaatccttt gttattaaaa acaaaggaag
     1321 ttatatagcc gatgttatta acttaaagat ggaacgaact ttaaaaaatc ctccaaaagc
     1381 atcaaacggt agaacggata gtatagcaac atttaactat tataaaaaac atttttggga
     1441 tgatgtaaat tttaaagatg aaggcattta tagaaatcct ttttttcacc tgaaaattaa
     1501 aaaatatctt gaccaagtag tatatccaca tccggacacc ttgatagtgg ctgtggataa
     1561 aattattgaa caaacaacac ctggaagttt attgaataaa ttgttaattg gaaacttaac
     1621 ttatacttac gaatcatcaa aaaaaatggg atttgataaa gtatttgttc atctttcaga
     1681 taaatatttc aaaacaggaa aagcagttgg gatttataat gatgattcgg ttattcaaaa
     1741 aatcatcaaa cgtgccgata agttaaaacc cataactgtt ggtaagattg cacctgactt
     1801 atcaatgatt gatgtgaaaa atggtccgag aatgaagcaa cttggctttg aaaaagcaaa
     1861 cactagtgaa gaagtgacca aattatttta tgacaatact aatgagttga ataaaatatt
     1921 tgtaaaacta catgatgtaa aagcggaata tactattctt attttttggg atgtagattg
     1981 tggtcattgt caaaaggaaa ttcctgtttt actggatgtt taccacgaat tgctgaaaga
     2041 aaagaaagat gtaaaagtat atagtgttta cacgctattt gattctgaga aatatcaaaa
     2101 atacattgac gaacacaaac ttgattttat caatctatat gatgcatcac atttgaataa
     2161 tgttatcgaa atatatgatg tttattcaac acctgtaatt tatatattgg ataaagacaa
     2221 acgcatcaaa gcaaaacgaa ttggtgttga gcaaataaaa cctataatta cagctttaga
     2281 aaacgaggca aagtcaagca aatcttaaaa ttagccttca gtttgaaact tggcacgttt
     2341 acttccttca tacatttcat atttcactaa tcgtgcttcc aaactaccgt tgaaaagttt
     2401 tatttttcgt gaaggtcgaa gtccaacaaa ttttagcgca tcaagattgg cggtaataaa
     2461 ccaagcgttt gtattagaat agttattctt taatgtatcg cccaattctc tataaaaacg
     2521 ttccaagtct atatccaatc gttcaccgta tggtggattg aaaaccatgt gtaatggtcc
     2581 aacattctct ttttttgatt caaaaaagtt ggcttggtga atggttacat aatcttcaag
     2641 attagcgttt aagatattgt cttttgcttt ttgaaccgca cttggtgctt tgtcataacc
     2701 cgttattgta tatctaaatt ctttagtacg tttcaataac gcatcgataa tttggtcaaa
     2761 caaatcattg tcccaatcgt tccatttttc gaaagcaaat tcttttctat tgatattagc
     2821 cggaatattg caagcaatca ttgctgcttc ggcaagtaag gtgccacttc cacacattgg
     2881 atccataaaa tcagaacttc catcccaacc cgatagcatc agcatacctg ctgccaaaac
     2941 ctcatttatt ggtgcaatat tagtagctgt tctataacca cgttggtgta atgattcacc
     3001 agaagaatct aatgaaacag tgcattggtc tttatcgata tggatgttaa tttttaaatc
     3061 tggaaaatct ttatcaacac taggtcgaac tccttttttg actctaaatt gatcaactat
     3121 ggcatctttt gctttttgag aaacaaattg cgaatggtta aaatgatccg aatgaatggt
     3181 cgtgtcaata acaaatgttt ggttttcatt caaatacttt gaccaatcaa ttccctgaat
     3241 gcctttgtac aagcttaaat cgttgaacgc tctgaattga tagattggtt ttaaaatctt
     3301 taatgccgtt ctaagcgata aattggcttt atacataaaa cctttatcac ctttaaaact
     3361 tacagcacgc gttccaattt ctacattttg cgcacctaaa atttgtagtt ctttggcaag
     3421 tatttcttca aaaccaaaaa aggttttagc taacatttta aaattctcca ttttcaatat
     3481 caatatcaat gacaagtcca attacaatag caaattcaat tgcaataaca ctaatcaatt
     3541 gttaaaactt gcgcaaaaat acattaaatt tgcgggttca gaattcattg aaaagaataa
     3601 atgtcagaaa taaataaaac aacaaccaaa aattggtttg tttcttggtt tgatagtcca
     3661 tattatcata tactttacaa ggatagaaat tatcgtgagg cgcaactatt catggatacg
     3721 ttgacacatt atctcaattt gcccgaaaaa gcaaaagtgc tcgatttagc atgtggcaaa
     3781 ggcagacatt ccatttattt aaatcaatta ggatttgatg tcattggtgc cgatttatcc
     3841 gaaaacagca ttgaagaagc aaataaaaat gctaatgaca atttgcattt tctagttcat
     3901 gatatgcgtg agtcgtatga agaaaagttt gatgctatct ttaatttgtt taccagtttt
     3961 gggtattttg aaaacgaaca agatgattta aaaacgctgg ttgccatcaa agaaagtctt
     4021 gctgaatatg gttttgctgt gattgatttt atgaattcct atcaagtaat tgaaaacttg
     4081 attcctgaag aaaccaaaac cgttgatggt attgattttc atatcaaaag atacgttcat
     4141 gacggtttca tcatcaaaga aattgatttt gaagataaag gagaaatatt tcattttgtt
     4201 gaaaaagtta gaggatatac cttagaagat ttcaaacgct tgatggaaga agctggaatt
     4261 taccttttgg atgtctttgg cgattataaa ttgaagaagt ttcacaagaa tacaagtgaa
     4321 cgtttaatta tgatttttaa gtaatttgga agatggaaat tgtaagttga agatacaaat
     4381 cagaactatg cacaaacaat cttaaaccga tttaaactat tttaaacaat cttaaactgt
     4441 tttagaccac aatgaactat atacttccct tactatcagt gctaattggt tatgtgtttg
     4501 ccatttccat caaaccaaaa gacaagaaga acttaaaact tcttttggcc tttagtggtt
     4561 cttttctatt atcattaacc gtattgcaat tgcttcccga agtttataaa aacccaacaa
     4621 aacgcattgg aatttttatc atggcgggaa tattattcca aattatcttg gagttttttt
     4681 ccaaaggtgc cgaacatggt cacgtacatg gtcatgacaa gattcatcaa atgccttggt
     4741 tattgtttgt cagcttgtgt cttcatgcgt tgttagaagg tttcccagtt ggtcatcatc
     4801 acgatttagc tattggtatt gccattcatc atttaccgat tgccattatc ctaacgactt
     4861 ttttcttgaa tgcaggttta aacaaaactg ctttactcct attcatgatt acatttgctt
     4921 taatgacacc aattggaacg tttctttcag atacatttcc catcttgaat gactattaca
     4981 gagaaataac agccgtagtt attggtattt tgtttcacat ttcgtcaact attatctttg
     5041 aaagtagcga aggacacaaa ttcaacattg ctaaagtttc catgattatt cttggaattt
     5101 tattggcgag ttttttgtaa aactttgtaa ctttgagatt ttgctaaaat aaactatgaa
     5161 cttcaccaaa accactgaac aatcttcgca ttatgaacat ttagaacaaa tgtcggttaa
     5221 agaattgttg accaatataa ataacgaaga taaaacagta ccacttgctg ttgaaaaagc
     5281 actgccacag atagaagcat tagtagctaa aacagttgag aaactaaaac ttggcggaag
     5341 attgttttat atcggtgctg gaacttcggg ccgactggga attgttgatg cttcggaatg
     5401 tccgccaaca tttggtgttc cgtttgaatt ggttaatgga ataattgctg gtggcgacaa
     5461 agccattaga aaagcagttg aaaatgccga agacaatagg aaacaagctt ggatagattt
     5521 gcaaaacgaa accatttctg ccaatgatgt ggtgattggc attgcagcat cgggaactac
     5581 accttatgtt attgcaggat tagaaaaatg caatgaaaat aacattgtaa caggttgtat
     5641 aacatgtaat gaaggaagtc cattggcatt gaccgcaaag tatcctatag tagtcgtggt
     5701 tggaccagag tttgtaacag gaagttcacg tatgaaagct ggaacggctc aaaaactagt
     5761 tctgaatatg atttctactt ccgtcatgat acaacttggc aaagtaaaag gcaacaaaat
     5821 ggttgatatg caattgagta atgacaaact agttgaccgt ggcgtgaaaa tgattatgag
     5881 tgaaattcca gtagattatg aaacgggaag taaattgcta aaagtacatg gaagtgttcg
     5941 aaaagctgtt gataatttta aaaaataata gtatgaaaaa gttagtattg attttagtat
     6001 tgctttttgc caatactgtt ttctcccaac aaataaagct tgagaaagga aaatactttg
     6061 tagatggaca gcaaatttct actcgcgaaa cacgacaatt attactaacc gactacaaag
     6121 caactcgatt gtttcaaaaa gccaaatcca aagaaggtat tggaggtttg ttgcttggtg
     6181 caggaattgc aatgaccgta actgatttgg caataggact gttttccgat gtgaaatatc
     6241 caactgcaat aacctatgta ggagtggcaa ccatggctat ttcagttccg atactttcgg
     6301 gaagaaggaa aatgattaaa gaagccattg atagttataa ccaaacagca aaacaacttg
     6361 gaagcaacga tgatagtttc aaatatcaaa tagttgccaa tcaaaatgga attggaatac
     6421 aattaaaatt ttaattatgc caaccaacag aaacttactc aacaaaggaa ttaaatactt
     6481 ggtctattcc attccgctca tttttattgg tccaacggta atctataatg cgtttatgaa
     6541 caagcatacc aattggcatt atttagtcct agccattgga tgcctcatgt gtttaggagc
     6601 catgttcttt atttttttag gtattaaaac cataacagat tcacttttca acgatgataa
     6661 ataacaatta cctcgaaagc atcaaaaaac aaatgcttta ttataaaaca ttagccgaaa
     6721 aggctattga ccaacttgaa gaagcacaat tatttgtttc gaataatgat gacaccaatt
     6781 ctattgcagt aattgtaaat cacatgtcgg gaaacatgct gtcaaggtgg acagatttct
     6841 tgactactga cggcgaaaaa gaatggcggc aacgcgatgc tgaatttgag aatagcatca
     6901 atactaaaga gacacttctt gctacttgga acaaaggttg ggattgcttt tttaatacta
     6961 ttaatgcttt agaagctgat gatttagaga aaatcatcta tattcgcaac gaaggacaaa
     7021 cggtggtgga agccatcaac cgacagttgg cacattatcc gtatcatgtt gggcaaattg
     7081 tcttttatgc caagatgttg aaacaggatt cgtggagttc gctttcaatt cctaaaaaca
     7141 attctcaaag ttataatgcc gaaaagtttg ccaaagacaa aaccatcaag cattttacag
     7201 atgatgaact tactaaatta caatagcaat cagaaaatga aatctttcct taccaactac
     7261 cttttcccta ccgttttatt gcttctctta tcttcttgct accaacaaga acgaaaatgt
     7321 gccgacttta aaacgggaac atttcaattt aaacaagaaa ttgatgggaa agagcacacc
     7381 actactttca ttagaaccaa cgatatgcaa attgaaacct ttaatgggaa aactgatacc
     7441 gctactgtcc gttgggtgaa tgattgtgag tttattttag aaaaattgca ccctaggaac
     7501 atgcaagaaa aaaaagccat ctctatgaag attttgtaca ccaaagacaa cacttatact
     7561 tttgaatacg cttttgtagg cgatgaaaaa aaaatgaaag gtttagtaac aaaactagaa
     7621 taattacgtt taactgttaa aattcaaacc caataaatgg aaattttatt tacaccaaat
     7681 gcaataatcg cgttattaac actaaccttt cttgaaatca ttctaggaat agacaacatc
     7741 gttttcctat ccatcgtttc tggaaaactg aaagaagaag atcaacccaa agcccgaaga
     7801 attggattat tattagccat ggcgtttcgt attatattgc tttttggaat tacttgggtg
     7861 ttgagtttac aagacacgct tttatccatt gattggagtt tcttttcggc acatattact
     7921 ggacaaagtt taatcatttt tggtggtgga ttgtttttgt tgtacaaatc ggtttcggaa
     7981 attcatcata aactcgaagg tgaagaagaa agcgaaaaag gcaaatcatc caacactatt
     8041 tcgcaagcga ttatacaaat tgcattgctg aatatcgttt tctcttttga tagtattcta
     8101 accgctgttg gtatggtaag catgaaagaa ccagccgaag gtggttttgg ttatgaaggt
     8161 gcgctgttta ttatgatttt atccgtggtc atttctatca tcatcatgat ggtttttgcc
     8221 ggaccagttt ctaagtttgt aaacgaacat ccaacgatac aaatccttgg cttatctttt
     8281 cttattctga ttggtgtgat gttacttgcc gaaggttcgc atttggcaca cttccgtttt
     8341 ggaaatgata ttgaagttaa tagtatcccg aaaggttatt tgtattttgc tattttcttc
     8401 tcattatttg tggagttctt gaatctaaaa atgaagaaaa gtaaaaacaa agttaaactg
     8461 cacaacagta ctatagtgga cgaaaaactt aaagacggcg atgctacttt gtaaaaatgg
     8521 tgactaatgg ataacaaaac gttgtttacg catcctatct tgcgcacagt ccttattttt
     8581 ggaggattgt ggctcatcac taccgtttta gtaattgcag ttattaccaa catgtttgta
     8641 gaaccgatta tccaaccgaa atactttgtg ctctacatgc ttttgtttgc ttctacgagt
     8701 acaatggtaa cattgataaa aaactatata aaaactagaa agtaaatttt tataactaga
     8761 gcagtatatc tttcaaaaat ttcttaaagt agcattttaa aaaaatgaaa aatttaatta
     8821 tttaaaaata ttcatttcct catcataaat tgggctttca atacttaaaa aattcaaaac
     8881 actatgaaaa acatgatatt gagtcaattc tttattagct ttgagttgtc ttgaattatc
     8941 agaaatccaa acgatgaaag gaatctcata ttgttctttg ggagcaatac tcataggaat
     9001 tccgtgcata tatagatttt tttctcctaa agattctcca tgatctgaaa cgaaaagcat
     9061 ggtgctttta taatccttta attctttcaa atcttcaatt acttttgata gaatataatc
     9121 tgtgtaaaca attgtattat cataagcatt gattagttcc tttttagagc aatttccaag
     9181 ctcaacacta ttacaaacgg gtttaaaagt ttcaaattga ggtggatatt tcttgctata
     9241 cgttggtccg tgactagtgc tggtgtgcaa aactattaat attttatttt ttgtactggc
     9301 gcgtatttgc tcttttaatc ctgttaaaag tacttcgtca tacccacatc cttcaccttt
     9361 acaatttggt cttaagacat cttccttctg atagtttttt atatgaactg gaggttctcc
     9421 ccaatttgaa gttctccaaa taacttcaac gttattgcga tataaataat ttggcaatat
     9481 ttcatacagc tcatctgtat ttttataatc caaaatagct ttaactccag cggttgtata
     9541 ggtagccgaa gatattgcat tgaaatgaaa tacattagta attttagaaa gcaatggatt
     9601 ggtgttttta tcatatccat ataaagaaaa attttgactt cttgccgatt cgccaataac
     9661 taaaaccaca accgattttt cgttgttttt gactatagca tttggcaaca aaatttcttt
     9721 ttgatttttc ttaaattcgt gtgcataaaa aagagaaata tttacagtat aagaccaagg
     9781 cattgctaaa ccacccaatg tttttgaatt tttatcaatc caaagccaat tgctagcatt
     9841 agcaaaagct aacgcaagaa taaagattaa ggttagcgaa attgtaatta aaaacttttt
     9901 ccaagtatct tttattattt taactttgaa aatgtatatg cttggtataa ctccaaatag
     9961 aactaagtag gctatcaact ttaaggaaaa aaagctactt gcttctccat atttagtatt
    10021 aagtacattc cctatcatac tttcatcaat aataacacca taagtattta caaaataaac
    10081 ggcaattgca ttgatgcaaa aaaatagtac taatagaaac tttccgacac gacgagataa
    10141 ggaa
//
LOCUS       DAOOBH010000083        10067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_8365_length_10067_cov_4.534359, whole genome shotgun sequence.
ACCESSION   DAOOBH010000083 DAOOBH010000000
VERSION     DAOOBH010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10067
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8365_length_10067_cov_4.534359"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..2043
                     /locus_tag="PK218_08615"
     CDS             121..2043
                     /locus_tag="PK218_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW98609.1"
                     /translation="MKKSLLVLSVLFIAFSCKKETAPIDTIQTQNQKNIQKWFNASIS
                     LSKGTPDTIAFYANKMEQAALKEPSVYKAMVALIKGIEYANTSSYAISTKYYDSALRL
                     LKTTTADTLWAKAYNGLGNNYKITGDYTAALKNLFKALAVYEKKQDKIGICNVHAGLG
                     EVYFQKDNLGDAKKHLTIAMNTLAHDKSNQAYLSAAHILANVYGMSGDFDSALKIDEV
                     GFKITDSINNPKIKSSFLDNKANCFLFSNRLDSAQYYFNECLKIDLAGGNKKQLADTY
                     SNLGELFLMKNNFAKAEEYALKSVGVLKSIDGKPNLGKSYGILTEIYTKQRKFEKALE
                     IQKLQFENKSQMINLKKEASEAEYKIVYETQKKENKIQFLQEEEKIKELKIKQQHYQI
                     ERRNFLIVFFILLIGVLIGVGYLWRSKQKLRDKIRKNKIIQKTEEQIRLKMAKDIHDD
                     LGSGLSKINFLSEIISQKTKDIPEIISSSESIRETSKSMIDNMRDLIWALNPENTTLS
                     NFIARIREYTTDYVEDYSIETQYDIPENIAPTPIKNEMHRALFLVVKETINNIAKHSK
                     ATKIEFSAHISPEYLTLTIQDNGVGFEDSNTNGNGLKNIKSRIEDIGGKLTIESTIGA
                     GTTVIIKIKLDKIYKK"
     gene            2164..2790
                     /locus_tag="PK218_08620"
     CDS             2164..2790
                     /locus_tag="PK218_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW98610.1"
                     /translation="MTIKVGIVEDEKQIRESLMVLINGSEGFSCVHTFEDAESAIASI
                     PTLDLDVVLMDIHLPVKDGIACVQELAVINSAVNYVMCTSFEDTDSVFRALKSGAKGY
                     ITKTTQPSKILDAIVEVYHGGSPMSSNIARKVVASFHKQQQETAEMKKLTEREKEILH
                     LLSEGLRYKEIADKLFLSTETVRTHIRNIYEKLQVNSRTEALNKTFPK"
     gene            2906..3655
                     /locus_tag="PK218_08625"
     CDS             2906..3655
                     /locus_tag="PK218_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98611.1"
                     /translation="MKNTKSILAVLTISVFLSIVCISFNSCSKDSSSTPSYECTTCAS
                     TPDALAENDGSAKGVYKGIVVGSSGSLYIDIQNGTDLITGTLIIDGVSVALTSSVSYV
                     SGQPYVAPFTGIYNGSPISITFSVGLSGGTPTVTSSDIPGHPNTVFTLYKETSTSLIE
                     AFEGGYTKPGKTGTFNILLSRGLNLWGGIAKGNEAGAEVDEIDGTIDSSNRLIESSNG
                     TILGTLSGDEINGSFQDGNGDTVTIHGERTL"
     gene            complement(3772..6207)
                     /locus_tag="PK218_08630"
     CDS             complement(3772..6207)
                     /locus_tag="PK218_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW98612.1"
                     /translation="MKLKDFSLLLLLFFTANTFAQFTINGVVLGADGKKVAAVAVYNK
                     TSATKTSTNSNGEFSFEVDENKNYELVFYKGNYGIVEKTVTTSDSNLQIVLEKITALS
                     EVVINKQRDKVFALNKLRDIEETAIYAGKKTEVIAVNKLTANKSTNNTRQIFAQVVGL
                     TINESSDGGLQLSIGGRGLNPNRTSNFNTRQNGYDISADVLGYPESYYATPTEALEEI
                     QIVRGAASLQYGTQFGGLVNFKIKKPPTKPFELITRNTVGSYNLVTNFTSISGTNGKF
                     SYYSFYNFKQGDGFRPNSDFNSKNYFANLNYQFTSNTSLHFDYTYFNYLAQQAGGLTD
                     VMFNENPEQSNRSRNWFAVNWNLFALRLKHKFANNADFSLQLFGLDASRKTVGYRSNR
                     VSSEDIDGTVRDLIAGDFVNYGAEARYLKRYQIKGNNSAFLIGAKYYQSRNTGIQGPG
                     SAGSDANFHLATDDFPFYANQSDYVFPNINISLFGENIIKVSPKFSITPGFRFEKIRT
                     KAEGSYRKINLDLAGNVILDETIVENNDKDRNFLLLGIGFSYKPQNRIEFYGNVSQNY
                     RSVTFNDIRTVSPSQVIANDITDEKGYTSDIGVRGQIGNKITFDSSVYALYYDDKIGE
                     YEAQNPNGAAAVVRFRDNIGTAITYGFETMIDWSLSNTFFNQNEDFSWNLFTNTAITN
                     SEYLKSNAPNIEGNKVEFVPLYNIKSGSAIGYKNFMASLQFTYVSSQFSEANNSVTDT
                     NDNTYGIFGQIPAYYVADISTSYKWKRFKLEAGINNFTNNSYFTRRATGYPGPGIIPS
                     DNRTFYATLEFTF"
     gene            complement(6188..7552)
                     /locus_tag="PK218_08635"
     CDS             complement(6188..7552)
                     /locus_tag="PK218_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HPW98613.1"
                     /translation="MKHKLKTYFNSYTSTATLAFYRLAFGLMMLFSLIRFTALGWIEK
                     LYILPTNRFTYYGFEWIPRLGDYTYLLFLLAAIAAFCVAIGYKYRIAIITFFLCFTYI
                     ELLDKTTYLNHYYFISIVSFVLIFLPANAYFSVDAKQNPKLCFQYIPKWNVDILKVFI
                     AIVYFYAGLAKINSDWLLEAMPLKIWLPTNTDLPFIKTFLNQNWVHFAFSWSGMLYDL
                     AIPFLLLYKPTRKFAFVLVVVFHVVTRMLFPIGVFPFVMIVSSLVFFEPAFHENILRK
                     IAAFFKINMERFTNALYYETNRMASKAIKGFLIVFIGFQLVSPFRYLLYPNELFWTEE
                     GFRFSWRVMLMEKAGYTQFKVVDSKTKKEIRVNNSLFLTTFQEKQMAFQPDFILEYAH
                     ILKKFYEKQGVSNPQVYVESYVALNGRLSQKYIDPNVDLAKEKESFKPKYWIVPFNDE
                     IKGL"
     gene            complement(7555..8667)
                     /locus_tag="PK218_08640"
     CDS             complement(7555..8667)
                     /locus_tag="PK218_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HPW98614.1"
                     /translation="MKKLFLFVCLVSLIYACSSSDDGGSKTDNFDRTAMLTHWADNII
                     IPSYVDYDNKVLTLSSDVTNFTSTPTEANLQLVRTSWINAYKAYQKITIFNVGKAMEI
                     NLKETTNTYPTDAAGIETNIASGNYNLELLSQYSKQGFPALDYLINGLGTTDTAILAF
                     YTSNANATNYKNYLIDVTNKLKSNSNVVVADWNSSYRTTFISSSGNTVSSSVNKMTNL
                     FVKNLEKDIRSGKIGIPAGVFSSGTLFPEKVEAFYKKDISKDLLNIATQAQQDFFNGK
                     NFNSTVTGPSLKSYLDYVNAVRNGQNLSTIINNQFSSIYTANNLLDNNFSSQITSDNS
                     KMLAAYDALQQNVVYVKLDMMQALNITIDYVDGDGD"
     gene            complement(8861..>10067)
                     /locus_tag="PK218_08645"
     CDS             complement(8861..>10067)
                     /locus_tag="PK218_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4856 domain-containing protein"
                     /protein_id="HPW98615.1"
                     /translation="KQFLALLAFSSFLISCSNDDSSSATPEYTVPATYTFERNNATSI
                     DFSGQTTRIAMLQAMGDYYKTQANAGVAIDASALSNMYSNTGNPFSDTNLNTSGKQLK
                     DKTAASVDYFSLLYGGGTTAEQVSVRSFFENQFTEGNNASQGNNASAGVAGKYIDGSS
                     NRLFAANGLEPQQVLLKGMMGASFMDQIVNNYLSVNKLDAGSNKIDNTNKVLEAGKNF
                     TTMEHNWDEAYGYIYGADDATNNVYRFWSSYINQVNADTDFNTLKADIDLAFRKGRAA
                     IVANDYTTRDAQIAIIKEKLALVPAVRAVYYLQEGKEKLVTDNGAKAFHALSEGYGFI
                     MSLRYTNKPGTNNPYFTKAEVDTMLNSLLSGPNGLWDIDNLSPKLDAISTQIATKFGF
                     TVAQAATVD"
BASE COUNT         3450 a         1600 c         1739 g         3278 t
ORIGIN      
        1 tagagctgtt agtagcaaat taaaggagac gttatatatt tttgttttta tgaagttaaa
       61 aaaaaaaaga gttatgcaaa caaaaattgg agtacattta taaaagcaaa aattgttttt
      121 atgaagaaat ctttactagt actcagcgta ttgtttattg cattttcttg taaaaaagaa
      181 acggcaccta tagacactat tcaaacccaa aatcaaaaga acattcaaaa atggtttaat
      241 gcttctatca gtttgtcaaa aggtacaccc gatacgattg ctttttatgc caataaaatg
      301 gagcaagcag cattaaaaga acctagtgtt tataaagcca tggtagcgtt gataaaagga
      361 attgagtatg ccaatacttc ttcgtatgct atttctacaa agtattatga tagtgctttg
      421 aggttgttaa aaactactac tgccgatacg ctttgggcaa aagcctacaa cggattggga
      481 aacaattata aaattacagg cgattatacg gctgcactta aaaacttgtt taaagcctta
      541 gcggtttatg aaaaaaagca ggataagatt ggtatttgta atgtgcatgc aggtttgggc
      601 gaagtttatt ttcaaaaaga caatctcggg gatgccaaaa aacatttaac gattgctatg
      661 aatacgcttg cgcatgataa gtcaaatcag gcctatttgt cggcggcaca tattttggca
      721 aatgtttatg gtatgagtgg tgattttgat agtgcgttaa aaattgatga agtaggattc
      781 aaaataaccg atagcatcaa caaccccaaa attaaatctt cttttttaga caataaagcc
      841 aattgttttt tgttttctaa cagactagac agtgctcaat attattttaa cgaatgttta
      901 aaaatagacc ttgcgggtgg caataaaaaa cagttggccg atacgtatag taatcttggt
      961 gaattgtttt tgatgaagaa taactttgcc aaagccgaag aatatgcgct aaaaagtgtt
     1021 ggtgtgctta agagtattga tggaaaacca aacttgggaa aatcgtatgg aattttgacc
     1081 gaaatttata ctaagcaaag aaaatttgag aaagcattag aaattcaaaa attgcagttt
     1141 gagaacaaat cgcaaatgat taatctcaaa aaagaagctt ctgaagccga atataaaatt
     1201 gtgtatgaaa cccaaaagaa agaaaataaa atacaatttc tccaagaaga agagaagata
     1261 aaagaattga aaataaaaca acaacactat caaatagaac gaagaaactt tttgatagta
     1321 ttttttattc tgttgattgg agttttgata ggagtaggct atttatggag aagtaagcaa
     1381 aagcttcgag acaaaattag aaagaacaaa atcattcaaa aaaccgaaga gcaaattaga
     1441 cttaaaatgg ccaaagatat tcacgatgat ttgggttcgg gcttgtctaa aattaatttt
     1501 ctgagcgaga ttatttcaca aaaaaccaaa gatattccag agattataag tagtagcgag
     1561 tctattagag aaacttccaa aagcatgatt gacaatatgc gcgacttaat ttgggcgtta
     1621 aatcctgaga acactacttt atctaatttt atagcccgaa tacgagaata caccaccgat
     1681 tatgtagaag actattcgat agaaacacag tatgatattc ctgaaaacat tgcgccaaca
     1741 ccaattaaaa acgaaatgca tcgcgcattg tttttggtgg taaaagaaac catcaataat
     1801 atcgcaaaac attcaaaagc cacaaaaatt gaattttcag cacacatttc tcccgagtat
     1861 ttaacactaa cgattcagga caatggagta ggttttgaag attcaaatac aaacggaaat
     1921 gggttgaaaa acataaaaag tagaatagaa gatattggcg gaaagctcac cattgaaagt
     1981 actattggag caggaacaac agtaattatt aaaataaaat tagataaaat atataaaaaa
     2041 taagtaaaat ttgctgtgat ttacttttgc agattactac aaaagtagta gtgttacttt
     2101 ctgtaaataa gtaactttga caatacaaat gaagttttta tttttaagtc ttaatttttt
     2161 tcaatgacta taaaagtggg catagtagaa gatgaaaagc aaatcagaga aagtttgatg
     2221 gtgcttatta atggcagtga aggattttca tgcgttcata cttttgaaga tgccgaatcg
     2281 gctattgcca gtattccaac tttagatttg gatgtggtac tgatggatat tcatttgcct
     2341 gtaaaagatg gtatagcctg tgtgcaagag ttggcggtta ttaatagtgc cgtaaactat
     2401 gtaatgtgta catcatttga agacaccgat tccgttttta gagctttaaa atcaggagca
     2461 aaaggctata ttacaaaaac cacccaacca tccaaaatac tagatgccat tgtagaagta
     2521 tatcatggtg gttcgcccat gagcagtaat attgctcgaa aagtagtggc atcttttcat
     2581 aaacagcaac aagaaactgc agaaatgaaa aagctaaccg aaagagaaaa agaaattcta
     2641 cacttattat cggaaggttt gcgctataaa gaaatagccg ataaactttt tttgagtacc
     2701 gaaacggttc gaacgcacat tagaaatatt tacgaaaaac ttcaagtaaa ttctcgtacc
     2761 gaagccttaa ataaaacatt tccaaaataa tcagtattta aaaattacta ctttttggtg
     2821 attgtagttt tttaattgat ggagtacttt tgattaagta atagcattac gcatttattt
     2881 aattaacctt aataacacaa ttaaaatgaa aaacacaaaa tcaatcttag cagtactaac
     2941 gatatcggta tttttatcga tagtatgtat tagtttcaat tcatgttcaa aagatagctc
     3001 aagcacaccg tcttacgaat gtacaacatg tgcttctaca ccagatgcct tagcagaaaa
     3061 tgacggaagt gccaaaggag tatataaagg aatagtagtt gggtcttcgg gttctttgta
     3121 tattgatatt caaaacggaa ctgatcttat aacaggaaca ttaataatcg atggtgttag
     3181 cgtggcatta acttcaagtg tttcttatgt tagcggacag ccatatgtag caccatttac
     3241 aggaatttat aatggtagtc caatatcaat tactttttca gttggtttga gtggcggtac
     3301 accaacagtt acttcttcgg atattccagg acatccaaat acggtattta cgctgtataa
     3361 agaaacatca acttcgctta ttgaagcttt tgaaggcgga tatactaaac cgggaaaaac
     3421 tggaacgttt aacattttat tgtcaagagg tttaaatctt tggggtggta ttgctaaagg
     3481 taatgaagca ggtgcagaag ttgacgaaat tgatggtact attgatagta gtaaccgatt
     3541 aattgaatcg tcgaatggaa ctattttagg cactttatca ggagatgaaa taaatggtag
     3601 ttttcaagat ggtaatggtg atactgttac tattcatgga gaaagaacat tgtaaaaaaa
     3661 aaaattttta taaaaccaaa acctcacaac gatgtgaggt ttttggtttt ataatgtgtt
     3721 tctacttaaa attttttaag gatttctata ataaaaaaag ctactaattt tttaaaaagt
     3781 aaattctaaa gtagcataaa acgttcggtt atcagaagga atgattcctg gtccgggata
     3841 gcccgttgct cttcgggtaa aatagctgtt gttggtaaaa ttgtttatgc cggcttctag
     3901 cttaaagcgt ttccatttgt aagatgtaga aatatcggca acataatagg caggtatttg
     3961 cccaaaaata ccgtaggtgt tgtcgttagt gtcggttaca ctattgtttg cttccgaaaa
     4021 ttgagaagaa acataggtaa actgaaggct cgccataaag tttttgtagc caatggcact
     4081 tcctgatttg atattataaa gcggtacaaa ctccacttta ttgccttcaa tatttggcgc
     4141 attagatttt aaatattctg aattggttat ggcagtattg gtaaaaagat tccaactaaa
     4201 atcttcattt tggttgaaaa aagtgttgct caaactccag tctatcatgg tttcaaatcc
     4261 ataggttatt gccgttccta tgttgtctct aaaccgaacc actgctgcag caccatttgg
     4321 gttttgggct tcatattcgc caattttgtc atcataatac aaggcataca cactagaatc
     4381 gaatgtaatt ttgtttccaa tttgcccacg aacgccaata tcagaagtat atcctttttc
     4441 atcggtaata tcattggcaa tcacttgact tggtgataca gttctgatat cgttgaaggt
     4501 tactgagcga tagttttgag aaacattgcc ataaaactct attcgatttt gtggtttgta
     4561 gctaaaccca atacccaaca aaaggaagtt tcggtctttg tcattgtttt ccactatagt
     4621 ttcatcaagg ataacatttc cagccaaatc gagatttatt tttcgatatg aaccttcggc
     4681 tttcgttctt attttctcaa atctaaaacc aggagtaatc gagaatttag gcgaaacctt
     4741 aatgatgttt tctccaaata aagaaatatt tatattcgga aaaacatagt cggattggtt
     4801 ggcataaaac ggaaagtcat cggttgccaa atgaaaatta gcatcgctac cagcacttcc
     4861 cggaccttga attccggtat ttcgagattg atagtatttt gcaccaatca aaaaggcgct
     4921 gttgttacct tttatttgat accttttcaa atagcgtgct tcggcaccat aatttacaaa
     4981 gtcgcccgct attaaatcgc gaacggttcc atcaatatct tcactagaaa cacgattaga
     5041 acggtatcca accgtttttc ggctggcatc caaaccaaat aattgcaaag agaaatcggc
     5101 attgttggca aatttgtgtt tcaatcgcaa cgcaaataga ttccaattta ctgcaaacca
     5161 gttgcggctt ctgttgcttt gctctgggtt ttcattaaac attacgtcgg ttaatccacc
     5221 cgcttgctgc gccagataat tgaaataggt ataatcaaaa tgcaaagagg tattgctagt
     5281 gaactgatag tttagattgg caaaataatt tttgctgttg aaatcagaat tgggtctaaa
     5341 accatcacct tgtttaaaat tataaaacga atagtagcta aactttccat ttgtgccact
     5401 tattgaagtg aaattggtaa ctaaattata agaaccaacg gtgtttcgag tgataagttc
     5461 aaaaggtttt gtaggaggtt tttttatttt aaaattaacc aaaccaccaa attgtgttcc
     5521 atattgcaag gaagcagcac cacgaacaat ttgaatttct tccaaagcct cggttggtgt
     5581 agcataataa ctttcgggat aacccaaaac atcagcactt atgtcatacc cattttgacg
     5641 agtgttaaag ttggacgttc tatttggatt caaacctcta ccgccaatgc tcaattgcaa
     5701 tccgccatcg gaactttcgt ttatggtaag tccaacaact tgcgcaaaaa tttgtcgtgt
     5761 attgttggtc gatttgtttg ccgtaagctt atttaccgct attacttcgg tttttttgcc
     5821 ggcatagatg gcagtttctt caatgtctct taatttgttt aaagcaaata ctttatcgcg
     5881 ttgcttgttg atgaccactt cggaaagtgc cgtgattttt tctaaaacta tttgcaaatt
     5941 gctatcggat gttgtgacag ttttttcgac aataccataa tttcctttat aaaacaccaa
     6001 ttcgtagttt ttattttcat ccacttcaaa tgaaaattca ccattagaat tggttgaggt
     6061 ttttgttgcc gaagttttat tgtaaacggc tacggcagct acttttttac catcagcacc
     6121 caaaacaacc ccattgattg tgaattgagc aaaagtgttt gcagtaaaaa ataatagcag
     6181 tagtaaacta aagtccttta atttcatcgt taaatggtac tatccagtat ttgggtttaa
     6241 atgattcttt ttctttggct aaatctacgt ttggatcaat atatttttga cttaaccttc
     6301 cgttaagtgc tacgtaactt tcaacatata cttgtgggtt tgaaacgcct tgcttctcgt
     6361 aaaacttttt tagaatgtgt gcgtactcta aaataaaatc aggttgaaat gccatttgtt
     6421 tttcttgaaa agtggttaga aacagcgaat tgttaactct aatttctttt ttagttttgg
     6481 aatctactac tttaaattgg gtgtaacctg ctttttccat aagcatcacg cgccaagaaa
     6541 accgaaaacc ttcttcggtc caaaacaatt cattagggta gagtaggtat cgaaaaggag
     6601 aaaccaattg gaatccgata aacactatta aaaagccttt aattgcttta gaagccattc
     6661 tgttggtttc ataatataaa gcatttgtaa aacgttccat attgatttta aaaaaagcag
     6721 caatttttcg cagtatattt tcatgaaatg caggttcaaa aaacaccaac gaactaacta
     6781 tcatcacaaa aggaaaaact ccaataggaa acaacattcg ggtgacaaca tgaaatacaa
     6841 ccactaaaac aaacgcaaac tttcgagtag gtttatacaa taacagaaat ggaatggcta
     6901 aatcatagag cattccagac caactaaacg caaaatgcac ccaattttga tttaaaaaag
     6961 tctttataaa tggcaaatca gtattggtgg gtaaccatat ttttaaaggc atggcttcta
     7021 gtaaccaatc tgagtttatc ttagctaaac ctgcataaaa atacactata gcaatgaata
     7081 ctttaagaat gtcaacattc catttaggaa tgtattgaaa acataatttt ggattttgct
     7141 tggcatccac cgaaaaataa gcatttgcag gaagaaatat caaaacaaaa ctcactattg
     7201 aaatgaagta ataatgattt agataggtgg ttttgtctaa aagttcgatg taagtaaagc
     7261 aaagaaaaaa agtaataatt gcaattctat atttgtatcc aatagcaaca caaaatgcgg
     7321 ctatggcagc aagtaaaaat agcaaatagg tgtaatctcc aagtcttgga atccattcaa
     7381 aaccataata ggtaaaacga ttagtgggca aaatataaag cttttctatc caacccaaag
     7441 cggtgaatcg aattaaactg aaaagcatca ttaaaccaaa cgctaacctg taaaaagcta
     7501 gcgttgcagt tgatgtgtat gaattaaaat atgtttttag tttgtgtttc atgactaatc
     7561 accatcacca tctacataat caatagtaat gtttaaagct tgcatcatgt ctaattttac
     7621 atatactaca ttttgttgca aagcatcata agcagcaagc attttcgaat tatcagaagt
     7681 aatttgactt gaaaaattgt tgtctaatag attgttagca gtataaatac ttgaaaattg
     7741 attgttgatg atagtactca agttttgacc gtttcgaaca gcgtttacat aatctaaata
     7801 ggactttaaa ctcggaccag taacggtgct gttgaagttt tttccgttga aaaaatcttg
     7861 ttgcgcttgg gttgcaatgt ttaataaatc tttagaaata tcttttttgt aaaaagcttc
     7921 tactttctct ggaaataaag ttccactaga aaatactcca gcaggaatac caatttttcc
     7981 gcttctgatg tctttctcta aattcttaac aaacaagttg gtcattttat ttaccgaact
     8041 gcttacagtg tttccgctac tagaaatgaa agtagttcta taagaagaat tccaatcggc
     8101 aacaacgaca ttactatttg attttaattt gtttgtaaca tcaattaaat agtttttgta
     8161 attagttgcg tttgcatttg aagtataaaa agctaaaata gcagtatcgg tagtacccaa
     8221 accattgata agataatcta aagcaggaaa gccttgtttg gaatattgtg acaataactc
     8281 taaattataa ttacccgatg caatgttagt ttcaattcct gctgcatcag taggatatgt
     8341 gtttgtagtt tcttttaaat ttatttccat tgctttacca acattgaaaa tagttatctt
     8401 ttgatacgct ttataagcat ttatccatga agttcttact aattgaagat tggcttcagt
     8461 aggagtagaa gtaaagttag taacgtcaga agataaggtt agcactttgt tgtcatagtc
     8521 aacataactt ggaatgatga tgttgtctgc ccaatgtgta agcattgctg ttctatcaaa
     8581 gttatcagtt tttgaaccac catcatcact cgaagaacaa gcatatataa gcgatacaag
     8641 acagacaaat aaaaatagtt tcttcatttt aaattttatt ttttgcaaaa atacaaatgt
     8701 ttttattctt atctataatt attctaaata aatttaaaat aattttatca acaactataa
     8761 gactttgttg atgctatttt gatttaatta ttgagtaaaa aataacctca ataaattaaa
     8821 agtatattga ggttattatg tctctatttt taagaaataa ttaatctaca gttgctgctt
     8881 gagcaacagt aaaaccaaat tttgttgcaa tttgagtaga aatggcatcc aattttggag
     8941 ataaattatc aatatcccat aaaccgtttg gtccacttaa taatgaattt aacatggtat
     9001 ctacttccgc ttttgtaaaa tatggattgt ttgttcctgg tttgttagta tatcttaaag
     9061 acataataaa tccgtatcct tcagataagg catgaaatgc ttttgcaccg ttatcagtaa
     9121 ctaatttttc ttttccttct tgtaaataat aaacggctct taccgcagga actaaagcta
     9181 gtttttcttt aataattgca atttgagcat cacgagtagt gtagtcattt gctacaatgg
     9241 cagctcttcc ttttctaaat gccaaatcaa tatctgcttt taaagtatta aagtcagtgt
     9301 cagcatttac ttgattgata taacttgacc aaaatctata cacattattt gtagcatcat
     9361 ctgctccata aatgtaaccg taagcttcat cccagttatg ctccattgtt gtgaagtttt
     9421 tgcctgcttc taaaacttta ttagtattgt caattttgtt tgagccagca tctaatttat
     9481 ttacacttaa ataattattt acgatttgat ccataaatga agcacccatc attcctttta
     9541 ataatacttg ttgtggttct aaaccattag cagcaaaaag acgattagat gacccatcta
     9601 tatatttacc tgcaacacca gccgaggcat tatttccttg acttgcattg tttccttctg
     9661 taaattgatt ttcaaaaaaa gaacgtacgc taacttgttc tgcagttgtt ccgccaccat
     9721 ataataaaga aaagtaatca acagatgctg ctgttttgtc ttttaattgt tttcctgatg
     9781 tatttaaatt tgtgtctgaa aatggatttc ctgtattaga atacatatta cttaatgcag
     9841 atgcatcgat tgcaactcct gcatttgctt gtgttttata ataatcgccc attgcttgta
     9901 acattgcaat tctagtagtt tgtccagaaa aatcaattga tgttgcgttg tttctttcaa
     9961 aagtgtatgt agctggaact gtatattctg gagtagcaga tgatgaatca tcatttgaac
    10021 aggatattaa aaaagatgaa aaggctaata aagctaaaaa ttgtttt
//
LOCUS       DAOOBH010000084        10028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_8400_length_10028_cov_5.181691, whole genome shotgun sequence.
ACCESSION   DAOOBH010000084 DAOOBH010000000
VERSION     DAOOBH010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8400_length_10028_cov_5.181691"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1485)
                     /locus_tag="PK218_08650"
     CDS             complement(232..1485)
                     /locus_tag="PK218_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HPW98616.1"
                     /translation="MNIKMRLTIMSFLQFFVWGAWLITVGNYWFGTKQWSGEQFGAIF
                     STLGLSSILMPAITGIIADKWINAEKLYGVLHILGGLALLYIPQVDNPTAFYWVIFAA
                     MLCYMPTISLSNSVAYNILKNNEFDVVKVFPPIRVWGTVGFIAAMWLTNLTGNKASVN
                     MFYISAFAAFALGFYSFSLPKCPPQKLISQEASLIEKLGLNAFKLFGTYKMALFFIFS
                     MFLGGALQLTNMYGDVYLSEFAKIPEYADSFVVKYSTIIMSISQISETLFILAIPFFL
                     KRFGIKQVMLISMFAWVLRFGLFAYGNPIDGLWMIIVSCIVYGMAFDFFNISGSLFVE
                     TTTDSSIRSSAQGLFMMMSNGFGAFFGGLLSGVVIDEFFTHDGVRDWQNIWLSFAAYA
                     LVIAVLFAIFFKHKHDPKAVENIAH"
     gene            complement(1616..2299)
                     /gene="cmk"
                     /locus_tag="PK218_08655"
     CDS             complement(1616..2299)
                     /gene="cmk"
                     /locus_tag="PK218_08655"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPW98617.1"
                     /translation="MKKITIAIDGFSSTGKSTLAKQLANHLGYVYVDTGAMYRAITFF
                     AMQNGYITSDYFDKETLINSLPFIKLQFKFNPNVGFAEMYLNGENVENQIRTMEVSGN
                     VSRVAEVSEVRAKLVEQQQDFGKEKGIVMDGRDIGTVVFPNAELKIFMNASMETKAQR
                     RFDELMTKGQEVTFDEVLKNVQERDYIDTHRKDSPLLKATDAIEIDNSNLNREEQFQI
                     VLDLVNQKM"
     gene            complement(2309..3340)
                     /gene="porQ"
                     /locus_tag="PK218_08660"
     CDS             complement(2309..3340)
                     /gene="porQ"
                     /locus_tag="PK218_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HPW98618.1"
                     /translation="MIKKIACTVILLSHLTIYAQVGGKSVYQFLNLVTSPRQAALGGK
                     TITIFDSDVNQAHFNPATINPEMDNKLSLNYGSYYGDVTYGTAAYAYTYDRHLQTFHA
                     GVNYVNYGKFDGYDENGQPTASFSGSDIALSFGYAYNIPYSNFYVGANAKLISSTLES
                     YNSFGAAVDLGALFIDEPNDVNWALVIRNIGTQITPYAETREKLPLEVLIGVSQLMEN
                     VPIRWHLTLENMQQWQVAFSNPNRAQGSLDGGSSAEKISIFNNALRHIVVGAELFPDK
                     GFNIRIGYNFRRAEELRILEQRNFSGISVGFGLKIRKLKFNYSYSRYTLAANTSLFGL
                     TIDFSDNSF"
     gene            complement(3462..5912)
                     /gene="lon"
                     /locus_tag="PK218_08665"
     CDS             complement(3462..5912)
                     /gene="lon"
                     /locus_tag="PK218_08665"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPW98619.1"
                     /translation="MSNHNIISIDNLSLQELDTDAELIPLLTPEDEEEMSKEELPDSL
                     PILSVRNTVLFPGVVIPISAGRDKSIKLINDANAGNKIIGVVAQKNEEIEDPDKNDIY
                     PIGTVAKILRVLKMPDGNITVILQGKKRFEIDSVVSELPYIKATVKEVEEVRPNSIDT
                     EFSAIVDSIRELAIEIIRESPNIPTEATFAIKNIESQSFLINFVSSNMNLSVKEKQDL
                     LMINALKERALETLRFMNVELQKLELKNDIQSKVRFDLDQQQREYFLHQQMKTIQEEL
                     GGVSNEQEIEDMRQKAKTKSWNEKVSKNFDKELSKLQRMNPQAPDFGIQRNYLELFLE
                     LPWDHFSKDNFDLKRAQKIIDRDHFGLEDVKKRIIEHLAVLKLRNDMKSPIICLTGPP
                     GVGKTSIGKSIAEALGREYVRISLGGLRDEAEIRGHRKTYIGAMPGRIIQSLKKAGTS
                     NPVFVLDEIDKLSNSHQGDPSSALLEVLDPEQNSDFYDNFLEMGYDLSKVMFIATSNT
                     MSTIQPALRDRMEVIKMSGYTIEEKTEIARQHLFPKQLKEHGLTTKDLSIGKKQLEKI
                     VEGYTRESGVRNLEQKLAQVIRNAAKSIALEEEYNKKVTDEDIVKILGAPKLERDKYE
                     NNDVAGVVTGLAWTSVGGDILFIESLISVGKGTLTMTGNLGTVMKESATIALEYIKAN
                     AGLFGLDSEVISKYNIHLHVPEGATPKDGPSAGIAMLTSLVSLLTQKKVKKSIAMTGE
                     ITLRGKVLPVGGIKEKILAAKRANIKEIILCHENKSDVDEINQEYLEGITFHYVKMMR
                     EVIDFALTDHKVKNAKKL"
     gene            complement(6131..7462)
                     /locus_tag="PK218_08670"
     CDS             complement(6131..7462)
                     /locus_tag="PK218_08670"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HPW98620.1"
                     /translation="MKNIKIIGRLLLLFTAITFMSCENEPIDPAIDLTNPGNTIGSFT
                     AKIGSEDFNANQLIDAEYTDTALGTQLTIIGVTSTGKTMSITVMNPAVGTRTASSNEA
                     SLLTFDYAASANDIYSSYNTTTSQYSGVLTITQFDLITKKISGTFSYTGYGALSSATQ
                     IQVTEGNLINITFDDSTTGGNNGENPNTTPVFKADFNNSTWSTTQYAAEVSPNFIQIA
                     GFKTNGESFLFLVNASGVGTYPANENILAYTPPGSEFGYWSSNFNNPNENTGSITITN
                     INTVTKTISGTFNFKGYWSDTDVTNIPPVQFTNGVFTNIPYTDQVESGDTFFAKVNGS
                     EFVEDDLLVVELGINGQDFYSIGAQNAALNSMTVSVRTNIGVGTYPITGNNATDVVQI
                     IYDFENVDYRGVSGNVTITEKTATRLKGTFSCVTDGATPFTISEGTFDVAY"
     gene            complement(7504..8826)
                     /locus_tag="PK218_08675"
     CDS             complement(7504..8826)
                     /locus_tag="PK218_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW98621.1"
                     /translation="MPNLPKGHPKLLNAWAFYDWANSVYSLVIASAIFPIFYEMIFKD
                     SGSNYVNFLGIKCKDTAMISFVTAFAFLIVAVFSPILSGIADYIGNKKAFLRFFCYLG
                     GISCISLYWFDLNQLFLSYTFYFFGLIGFWASLVFYNSYLPDIAFPEQQDRVSARGFS
                     LGYVGSTLLLIFNLAMVMFPHKFGITGTEKEASMFAMRISFITVGVWWIGFSHITYYF
                     LPKGNHKAKKITKDVIFNGFNELRKVWKQLQQNLALKRYLYSYFVFIMAVQTVMLVAT
                     YFGATEISWETSKQKTTGLIISILVIQLVAVVGAVVTSRASGKFGNIKTLLFLNAFWA
                     ILCAFAYFVETPNEFYITAGFVGLVMGGIQSIARSTYSKLLPETDDTASFFSFYDVTE
                     KIGIVIGMSIYGIIDQVTGSMRNAIVFLGVFFVIGFILLLRVPKKALI"
     gene            complement(8973..9794)
                     /locus_tag="PK218_08680"
     CDS             complement(8973..9794)
                     /locus_tag="PK218_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPW98622.1"
                     /translation="MKKGILFGAFVALLLVFSCAKNPFTGKSTMALVDNSQIFPSAFQ
                     QYNQFLTENKVISGTTDAKRVTDVGMKIKAAAEKWLNANGYTEYLKDYKWEYKLVDSK
                     DVNAWCMPGGKIVVYTGILPITKDDAGLATVMGHEVSHALANHGQQRMSAGMLQQAGA
                     AGVAIATGGKSEETQQLAMLAYSGVSQYGGMLPFSRSHESEADMIGLKLMAIAGYNPE
                     EAVNFWGRMSAASGGQKPAEFMSTHPSDETRIANLRRMIPEAKAEALKFGVTFKK"
BASE COUNT         3334 a         1813 c         1420 g         3461 t
ORIGIN      
        1 aacagtgttt ttatttaaat aattgatacc tagtggaaac tcttcttcgc caaaaggttt
       61 gcaaaagtaa tcataaatta ttaattacaa aaaaattata taaagaaatt gatggtgaat
      121 tgacggaaga tggaagttag gagctggaaa ttacttgttg gataatttca aataaaaaga
      181 cctaacaggt ctttaaaaac ttgttaggtc aaaatggcta taatttattt tttaatgcgc
      241 tatattttca actgctttcg ggtcatgttt atgtttgaag aaaatggcaa acaaaactgc
      301 aataactaaa gcataggctg caaaagaaag ccaaatattt tgccaatctc taactccatc
      361 atgagtaaag aattcatcta tcacaacgcc acttaaaagg ccgccaaaga aagcaccaaa
      421 accgttactc atcatcataa ataatccttg agcagaagaa cgaatacttg aatcggtagt
      481 cgtttcaaca aataacgaac cggatatatt aaagaaatca aatgccattc cgtaaacaat
      541 acaagataca attatcatcc acaaaccatc tattggatta ccataagcaa acaaaccgaa
      601 gcgcagaacc caagcaaaca tcgaaatcaa cattacttgt ttgatgccaa atcgttttaa
      661 gaaaaatgga attgctaaaa taaataaggt ttccgaaatt tgagaaatcg acataattat
      721 ggttgagtat tttaccacaa acgaatcggc atattcagga attttggcaa attcagataa
      781 ataaacatct ccatacatat tagttaattg caatgcgcca ccaagaaaca ttgaaaatat
      841 gaagaacaaa gccatcttat aagttccaaa taatttaaat gcattcaatc ctaatttttc
      901 tattaaagac gcttcttgag aaatcaattt ttgaggcgga cactttggta atgaaaaaga
      961 ataaaaaccc aatgcaaaag ctgcaaaagc tgaaatgtaa aacatattaa ctgaagcttt
     1021 atttcctgtc aaattagtaa gccacattgc tgcaataaac ccaactgttc cccaaactct
     1081 tattggtgga aaaactttaa caacatcaaa ttcattattt tttagaatat tataagccac
     1141 agagtttgac aatgaaatcg ttggcatata acaaagcatt gctgcaaaaa ttacccaata
     1201 aaatgcagta ggattatcta cttgaggtat atatagtaat gctaaaccac ctaaaatatg
     1261 aagaactcca tacaacttct cagcattaat ccatttatca gcaataattc ctgtaattgc
     1321 cggcattaaa attgaagaca aaccaagtgt tgaaaaaatt gctccaaact gttcgccact
     1381 ccattgtttt gtgccaaacc aatagtttcc aacggttatc aaccaagcac cccaaacgaa
     1441 gaattggaga aaactcataa tggtcaatcg catttttata ttcataacaa ataatttaat
     1501 agttttttgg taaaaaaata gctcgtaaat ctactattat aattttaaat tccaaatctg
     1561 gaattgaaat tacctttttt taaatcacaa attccaaata gtcaattttt aaacactaca
     1621 ttttttggtt tactaaatct aaaactatct gaaattgttc ttctcggtta agatttgaat
     1681 tgtctatttc gatggcatca gttgctttta acaatggtga atcttttcta tgagtatcaa
     1741 tataatcgcg ttcttgaaca ttttttaaaa cttcgtcaaa agtaacttct tgaccttttg
     1801 tcatgagttc atcaaatcga cgttgtgctt tagtttccat actagcattc atgaaaattt
     1861 taagttcggc attaggaaaa accacagttc caatatcgcg accatccatt acaattcctt
     1921 tttctttgcc aaaatcttgt tgctgttcta ccaatttcgc tctaacttct gaaacctctg
     1981 caacacggct tacatttcct gaaacttcca tggttcggat ttgattttcg acattttctc
     2041 cgttcaaata catttctgca aaaccaacat taggattgaa tttaaactgt aatttaatga
     2101 atggaagact attaattagt gtttctttgt caaaataatc cgaagtgata tatccatttt
     2161 gcatagcaaa aaatgttatt gcacgataca tggcaccagt atcaacataa acataaccta
     2221 aatgattcgc caattgttta gctaaagtgc tttttcctgt tgatgaaaat ccgtcaatgg
     2281 caatagtaat ttttttcaaa gtaatatatt aaaaactgtt atcagaaaag tctatggtca
     2341 gtccaaaaag acttgtattt gctgctaaag tatatcgaga gtaggaataa ttaaacttta
     2401 attttctgat ttttaatcca aaaccaacag atattcccga aaaatttcgt tgctccaaaa
     2461 tacgcaattc ttcagcacgg cggaaattat agccaattct tatattaaaa cctttgtcgg
     2521 gaaataattc ggcaccaact acaatatgtc gcaaagcatt attgaaaata gaaatttttt
     2581 cagcacttga accgccatcc aacgaacctt gtgcacgatt tggattggaa aaagctactt
     2641 gccattgttg catattttct aatgttaaat gccaacgaat tggaacgttt tccatcaatt
     2701 gtgaaacgcc tatcaatact tctaatggta atttttcgcg cgtttcagca taaggtgtaa
     2761 tttgtgttcc tatattccta ataactaaag cccaatttac atcgtttggc tcgtctataa
     2821 acagcgcacc taggtcaacg gctgcgccaa aagaattgta actctctaaa gtagaagaaa
     2881 ttaatttggc atttgcacca acataaaaat tggaatatgg aatattatag gcataaccaa
     2941 aagaaagtgc aatatcgcta cctgagaaac ttgctgttgg ttggccattt tcatcataac
     3001 catcaaattt tccataattt acatagttaa ctcctgcatg aaatgtttgc aaatgtcggt
     3061 cgtaggtata agcataagct gctgttccat aggtaacatc accataataa cttccataat
     3121 ttaaggaaag tttattgtcc atttcaggat taatagttgc tggattaaaa tgtgcctgat
     3181 tgacatcact gtcaaaaata gtaatagttt ttccacctaa agcagcttgt cgtggtgaag
     3241 ttaccagatt taaaaattga tacaccgatt tccctccaac ttgtgcataa atagtcaaat
     3301 gggaaagtaa aataacggtg caagcaattt tttttatcat aaaaaatata gtaatttata
     3361 tactataacg aaaaagcgaa gatattattt taaattataa gcacaaagtg aaaataaaaa
     3421 ggcgaaagta aaataaactc tcgccttttc ttcttattca attaaagctt tttagcattt
     3481 ttaactttat gatccgttag agcaaaatca ataacttctc gcatcatttt tacataatga
     3541 aacgtaattc cttctaaata ttcttgattt atttcgtcaa catcgctttt attttcgtga
     3601 caaagtatta tttctttaat gtttgctctt tttgccgcta atattttctc tttaatgcct
     3661 ccaacaggaa gtacttttcc acgaagtgta atttctccgg tcatggcaat acttttcttg
     3721 acttttttct gagtaagcag tgaaactaga gaagtcagca tggcaatacc tgcacttgga
     3781 ccgtcctttg gtgttgcgcc ttcaggtaca tgaagatgta tgttgtattt tgaaataact
     3841 tccgaatcca aaccaaataa accagcattt gctttgatat attccaatgc aattgttgcc
     3901 gattctttca tgactgtacc taaattccca gtcatagtca atgttccttt accaacagaa
     3961 attaaagatt ctataaataa tatatcacct ccaactgatg tccaggctaa accagtaacc
     4021 acacctgcaa catcattatt ttcatattta tcgcgttcta attttggtgc accaagaatt
     4081 tttacaatat cttcatcggt aactttttta ttgtattcct cttctaaagc aatagatttt
     4141 gctgcatttc gaattacttg tgctagtttt tgctctaaat ttctaactcc cgattctcta
     4201 gtatatcctt caacaatttt ttccaattgt tttttcccaa tacttaaatc ttttgtagtt
     4261 aaaccatgtt ctttcagttg ttttggaaat aaatgctgac gtgcaatttc ggttttttct
     4321 tcaatcgtat aaccactcat cttgattact tccatcctat cgcgtaaagc aggttgaatg
     4381 gttgacatgg tattggatgt tgcaataaac attacttttg ataaatcata acccatttct
     4441 aggaaattat cataaaaatc actgttttgt tccggatcta aaacttctaa taaagcagaa
     4501 gacggatcgc cttgatgtga attagataat ttatcaattt catccaaaac aaaaactggg
     4561 ttagaagttc ctgctttttt caaactttgg ataattcttc cgggcattgc accaatatag
     4621 gtttttctat gaccgcgaat ttcggcttca tcacgtaaac cgccaagaga aattctaacg
     4681 tattctctgc ctaaagcttc agcaattgat ttacctattg atgtttttcc aactcctgga
     4741 ggtccagtta aacaaattat tggagatttc atatcatttc ttagctttaa aaccgccaaa
     4801 tgttcaatga ttcttttctt tacatcttcc aatccaaaat gatctctatc aattattttt
     4861 tgagctcttt ttaaatcaaa attatctttt gaaaaatgat cccaaggcaa ttctaaaaat
     4921 aattcaagat aatttctttg aattccaaag tctggtgctt gcggattcat gcgttgtaac
     4981 ttcgacagtt ctttgtcaaa atttttgctg actttttcgt tccaagattt tgtttttgct
     5041 ttttgacgca tgtcttcaat ttcttgttca tttgaaacgc cacccaattc ttcttgaatg
     5101 gttttcatct gttggtgcaa gaaatattca cgttgttgct ggtctaaatc aaatcgtact
     5161 ttggattgaa tatcattctt caattctaat ttttgaagtt caacattcat aaagcgcaat
     5221 gtttctaaag ctctttcctt taatgcgtta atcatcaaca aatcttgttt ttctttaact
     5281 gaaagattca tgttggaaga aacaaaattg attagaaacg attgactttc aatatttttt
     5341 atagcaaaag tagcttctgt tggaatgttt ggactttctc taataatttc aatggccaat
     5401 tctcggatgg aatctactat agcagaaaac tcagtgtcaa tagaatttgg acgtacttct
     5461 tcaacttctt taacagttgc tttgatataa ggcaattcag aaacgacact gtctatttca
     5521 aatcgttttt taccttgaag aattacggta atatttccat ctggcatttt taaaacacgt
     5581 aaaatttttg ctactgtacc aatagggtaa atatcatttt tatcaggatc ttcaatttct
     5641 tcattttttt gcgctacaac tccaataatt ttatttccag cattggcatc gtttattaat
     5701 ttaatagatt tatcccttcc tgcggaaatt ggaatgacaa ctcctggaaa taaaacagtg
     5761 tttctaacgg ataatatagg taaagaatct ggtaattctt cttttgacat ttcctcttcg
     5821 tcctctgggg ttaataatgg aattaactcg gcatctgtat caagttcttg aagtgacaaa
     5881 ttgtcaattg agatgatatt gtggtttgac atgtattttt ttaatgaatt attatttata
     5941 tttctaataa acaccataca aatgtaatta ttagttttat tttattatag taataacgac
     6001 aaacaacaaa agttattgtc tatcgtaatt gtttttccgc tttatatgat gtactcgaat
     6061 aaatcaatga ctatgccata aaaaaaatcc gccaaattga cggatttcat tattttataa
     6121 aaaccattgt ttagtatgca acatcaaatg ttccttcaga aattgtaaat ggcgttgctc
     6181 catctgtcac acacgagaat gttcctttta atcgagtagc ggtcttttcg gtaatcgtta
     6241 catttcctga aacgcctcta taatcaacgt tttcaaaatc gtaaataatt tgaacaacat
     6301 ctgtggcatt atttccagtt atcggataag ttcccactcc tatgtttgtt ctaacagata
     6361 ctgtcattga gtttaaagcc gcattctgtg cgccaatact gtaaaaatct tgaccattaa
     6421 ttcctaattc gacaactaac aaatcatcct ctacaaattc agatccgtta accttagcaa
     6481 aaaaagtatc gccgctttcc acttgatctg tataaggtat gttggtaaat actccatttg
     6541 taaattgtac tggaggaata ttagtaacat cagtatctga ccaatatccc ttgaaattaa
     6601 aagttcccga aattgtttta gtaactgtat taatattcgt aattgttatt gaacctgtat
     6661 tttcatttgg attattgaaa tttgaagacc aatatccgaa ttcagaacca ggaggtgtat
     6721 aagctaatat attttcgttt gctgggtaag ttcctactcc actagcattt actaaaaaca
     6781 aaaagctttc accatttgtt ttaaaaccag caatttgaat gaagttggga gaaacctctg
     6841 cggcatattg tgttgtactc caagttgaat tattaaaatc ggctttaaaa actggggttg
     6901 tatttggatt ttctccattg tttccgcctg tagttgagtc gtcaaaagta atattaatca
     6961 aattaccttc ggtaacttgt atttgagtgg cacttgataa agcaccatag ccagtataac
     7021 taaatgtacc cgatattttt ttggtgatta aatcaaattg agttatcgtt aaaactccac
     7081 tgtattgcga ggttgtagta ttgtatgaac tataaatgtc atttgcactt gcagcataat
     7141 caaatgttag taaagaagct tcgttactac tagcagttct agtcccaact gctggattca
     7201 ttaccgttat agacattgtt tttcctgtag aagtaacacc aataattgtt aattgagtac
     7261 caagtgctgt atcagtatat tcggcatcaa ttaattgatt ggcattaaaa tcttcacttc
     7321 caatttttgc tgtaaaagaa cctattgtat ttcctggatt ggtcaaatca attgctggat
     7381 ctataggctc attttcacaa gacataaaag taatcgcagt aaaaagcaat agtaatcttc
     7441 caataatttt tatgtttttc ataactttaa aataattttt tccaaatata atttttttta
     7501 aatttaaatc aatgcttttt ttggaactct taataaaaga ataaatccaa ttacgaaaaa
     7561 tactcctaaa aacactatgg catttcgcat acttcctgta acttgatcta taattccata
     7621 aattgacatt cctattacaa taccaatttt ttcggttacg tcataaaaac taaaaaaaga
     7681 agctgtatca tcggtttctg gaagtaattt tgaataggtt gaacgtgcaa ttgattgaat
     7741 tccgcccata actaatccaa caaaaccagc tgttatataa aattcgtttg gtgtttcaac
     7801 aaaataggca aaagcacaaa gtattgccca aaatgcattt aaaaaaagta atgtttttat
     7861 atttccaaat tttcctgaag ctctagaagt cactactgct ccaactactg ccaccaactg
     7921 aattactaat atactaataa ttaatccagt cgttttttgt ttagaagttt cccatgagat
     7981 ttcggttgca ccaaaataag ttgctaccaa cataacagtc tgtactgcca ttataaaaac
     8041 aaaataactg tataaatatc tttttagggc taagttttgt tgtaattgtt tccaaacttt
     8101 tctcaattca ttaaaaccat taaagattac gtctttcgtt attttcttag ccttatgatt
     8161 tcctttgggt aaaaaataat acgttatatg actaaaacca atccaccaaa ctcctacagt
     8221 aataaatgat attcgcatgg caaacatact ggcttctttt tctgtaccgg taattccaaa
     8281 tttatgagga aacataacca ttgctaaatt aaaaattaac aacaaagtac ttcctacata
     8341 tcctaaagaa aaccctcttg cactaactct atcttgttgc tctggaaaag caatatctgg
     8401 taaatatgaa ttatagaaaa ccaaactagc ccaaaatcca attaatccaa aaaagtaaaa
     8461 agtatagctc aagaaaagtt ggtttaaatc gaaccaatac aaacttatac atgaaattcc
     8521 acctagataa cagaaaaaac gaagaaatgc ttttttgtta ccaatataat ctgcaattcc
     8581 tgataatatt ggagaaaaaa cagcaactat taaaaatgcg aaagcggtaa caaaactaat
     8641 cattgcggtg tctttgcatt ttattccgag aaaattaaca taattgctgc cgctatcttt
     8701 gaaaatcatt tcataaaaaa ttggaaaaat tgccgatgca atgactaaac tataaactga
     8761 atttgcccaa tcgtaaaatg cccaagcatt tagtaatttt ggatgacctt ttggtaaatt
     8821 tggcatgttt tcgatattag atattaaaat tttagacaca tcgactgtgc atagcattac
     8881 attttcaatt tttgcctttc gactatcagt ttatttcata aaaaaatccc gaaataaatc
     8941 gggattaaat atacattttt tttagtactt ttctattttt taaatgttac tccaaacttt
     9001 aaagcttctg cttttgcttc aggaatcatc cttctcaaat tggctattct ggtttcatca
     9061 ctcggatgcg tactcataaa ttccgcaggc ttttgtccac cagaagctgc tgacattctt
     9121 ccccaaaaat taacagcttc ttccggattg tatcctgcta ttgccattaa ttttaagcca
     9181 atcatatcgg cttcactttc gtgacttcta ctaaatggta acattccacc atattgtgaa
     9241 actccagaat acgctaacat agctagttgt tgtgtttctt cgctttttcc acctgtagca
     9301 atagcaacac cagcagcacc tgcttgttga agcattcctg cactcattcg ttgttgtcca
     9361 tggttggcta atgcatgtga aacttcatgt cccataacgg ttgccaaacc agcatcatct
     9421 ttagtaattg gtaaaattcc tgtgtaaaca acaatttttc cgcctggcat gcaccatgca
     9481 tttacgtctt tgctatccac cagcttatat tcccatttgt agtctttcaa atattcggta
     9541 tagccattcg cattcaacca tttttctgct gcagctttaa ttttcattcc aacatctgta
     9601 acgcgttttg catctgttgt tcctgaaatc actttgtttt cggtcaaaaa ttgattgtat
     9661 tgttggaaag cagatgggaa aatttgacta ttatctacta atgccatcgt gcttttacca
     9721 gtaaatggat tcttggcaca cgaaaaaact aaaagtaatg cgacaaatgc tccaaataat
     9781 attccttttt tcatgggtat aagtttttaa attaatacat ctcaaaaata agtagtcctt
     9841 aattcaaaag tgctacataa tttttaaaat caattacaac atatcaatgt atttagttta
     9901 caaaattaat aaactatgca ccaaatatgt gcaattctgt aaaatcttta tctattatta
     9961 agtaattttc tcctttaatt tgaagagtat ttaaatccaa tttttcttta tccacttcaa
    10021 ttttttta
//
LOCUS       DAOOBH010000085         9756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_8683_length_9756_cov_5.490465, whole genome shotgun sequence.
ACCESSION   DAOOBH010000085 DAOOBH010000000
VERSION     DAOOBH010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8683_length_9756_cov_5.490465"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PK218_08685"
     CDS             <1..950
                     /locus_tag="PK218_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW98623.1"
                     /translation="KTYTYILKKENGVVPDSLNTHTTITVPIKKIVVTSTTHIPSLEM
                     LEVEKTLVGFPNLDYISSEKVRALIDEKKIKELGANQALNIEVLIDLQPDVIIGYGID
                     NDTKSLENLENSGLKVLLNGDWNEETPLGKAEWIKLFGALYDKQDKAKKLFSIIEKDY
                     LNTIEIAKLDSVTPTILAGDMFQDKWYLPKGTSWGSILLKEANANYLWADTSGTGSLS
                     LSFETVYEKAKEADFWITSGQFSTLKEMMDANPHYEQFEAFKKGNVYSFAGKKGKTGG
                     VLYYELAPNRPDMVLKDLVKILHPDLLPGYEPFFFEKIK"
     gene            982..2007
                     /locus_tag="PK218_08690"
     CDS             982..2007
                     /locus_tag="PK218_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPW98624.1"
                     /translation="MSKNRNTLLFTSLSLVLLLTLVLNISIGQVTIPFKEVCKSLFGS
                     SASKETWEYIIINYRLPKAITAMLVGVSLSISGLLMQTFFRNPLAGPFVLGLSSGSSL
                     GVAFVVLGSSFLPIFLTDFLLSSHGIILASCAGSFLVLLLIFIVSKYLRDVMSILIVG
                     LMFSSFTSAIVSILTYFSTAAQLQKFTFWSMGSLGNLTWGNISFLTFVITIGLLISVF
                     SLKSLDALLLGENYAKSMGLNFKRFKNTIIISTSLLTGSITAFVGPIAFIGLAVPHLT
                     KLLFQTSNHKILFIGTLLIGAIIMLLCDSISQFPGMDFTLPINAITSLVGAPIVIWLI
                     IKKKNIL"
     gene            2010..2447
                     /locus_tag="PK218_08695"
     CDS             2010..2447
                     /locus_tag="PK218_08695"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HPW98625.1"
                     /translation="MKTISRNLILLLVLVSFNSCSELLDCIVRTEPVLPNKTLEIATV
                     GNNYNQYIIAEVKNDSNDDAYFYHFSINGDLPAGINYNISDRRISFYGVPTNPGTFRF
                     TVLLTIDYPNDSYYEDNDGIFIDDNRICFGDKRTSKTYEIKVQ"
     gene            2444..3235
                     /locus_tag="PK218_08700"
     CDS             2444..3235
                     /locus_tag="PK218_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW98626.1"
                     /translation="MSVNSNEILITKQLAVGYSSKKRTNVIANSINLTLQKGKLITLV
                     GANGIGKSTLLRTLTGIQKPLSGNVFLEDKNIQSLDDLTLAKNLSLVLTEKLPPSNLT
                     VFELVALGRQPYTNWLGKLSENDLKIINEVIAITQIEELLEKKHFEVSDGQLQKVMIA
                     RALAQDTSIIILDEPTTHLDLLHKVSVFKLLKKLSQETNKCILFSTHDIDLAIQLSDE
                     MIVMTQEKVYQDQPCNLISNGVFETLFKDEKISFDGTKGKFVISN"
     gene            complement(3222..3674)
                     /locus_tag="PK218_08705"
     CDS             complement(3222..3674)
                     /locus_tag="PK218_08705"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HPW98627.1"
                     /translation="MLNIVLVQPEIPNNTGNIGRLCVGTESRLHLIHPLGFHITDTNL
                     KRSGLDYWVHLDLTEYQNVDEWIAQIKDKSRVFLMSSRVDKSIYEAEFQDGDWLVFGK
                     ESIGLSEEVLDQFENHLKIPMSNLIRSYNIANSVAFVVGEAKRQINLK"
     gene            complement(3668..4249)
                     /locus_tag="PK218_08710"
     CDS             complement(3668..4249)
                     /locus_tag="PK218_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW98628.1"
                     /translation="MHRHFLFHKPHGYLSQFIYEKKRHKKLLGELYSFPEGTMAIGRL
                     DEDSEGLLLLTTDGMMSEIVRSKTVEKEYYAQVDGVITNKAIEQLKNGVEIGFKGIRY
                     TTKKCEAKIINQLPDCIGEGRRIRDERHGPTSWVSITLTEGKFRQVRKMTAAVGFPTL
                     RLVRIRVGTIHLQLKAGEVLEVASFSNNRIIKC"
     gene            4334..5698
                     /gene="rmuC"
                     /locus_tag="PK218_08715"
     CDS             4334..5698
                     /gene="rmuC"
                     /locus_tag="PK218_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPW98629.1"
                     /translation="MSDSLFIIIAFIIALAIGIYLGKMLFSANSKSEKASLEEKTNGL
                     LSQINQLKEQFQNERNQFEKQLFALNAEKETIRSEKEAFAIQLSKKETDFENLWERNK
                     EQKEEVEKLQEKFTKEFENLANKILEEKTSKFTEQNKENLKNILSPLQDKIQLFEKKV
                     EDTHKESIDYHAALRQQILGLREMNEQMSKETLNLTKALKGDSKMQGNWGELILERVL
                     EKSGLEKGREYEVQQSFTTEDGSRVFPDVVINLPDGKKMVVDSKVSLTAYEKYVNESD
                     DDEKGTHLKEHINSIKRHVEQLGNKNYHDLYQMESPDFVLLFIPIESAFALALNEDNS
                     LYNKAFEKNIVIVTPSTLLATLRTIDSMWTNQKQQENALEIARQAGALYDKFEGFVSD
                     LVKIGKKMDEAKTEYQGAMNKLVDGKGNLITSVEKLKKMGAKAKKALPENILIRAEKD
                     EQEL"
     gene            5682..6233
                     /locus_tag="PK218_08720"
     CDS             5682..6233
                     /locus_tag="PK218_08720"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPW98630.1"
                     /translation="MNKNFKTVAESQVTLSMLMLPSHSNFSGKIHGGFILSLLDQIAF
                     ASASKFSGNYCVTASVDTVDFLDPIEVGELVSLKASVNFVGRTSMIVGIRVEAENIQT
                     GKVKHCNSSYFTMVAKDDAGKPTQIPGLLIKSKQEYRRFLNALNRKKKQKEIDDYKVP
                     IDYNSEHLKKELSQYNIRIENFI"
     gene            6338..7756
                     /locus_tag="PK218_08725"
     CDS             6338..7756
                     /locus_tag="PK218_08725"
                     /inference="COORDINATES: protein motif:HMM:NF039230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98631.1"
                     /translation="MKKIYCLFIALSALIITFYACSDDNNYTPINTYPIATDDTVSNN
                     LINPVVIDVLLNDTTGDVPVASTVSIIGGTDTNSNGTLDNLVVPSEGTWSVNATSGMI
                     TFTPVPNYVGNPSPISYTVKNAQGNASNEATVTINATPIVSADIENIPYANLSEYHFF
                     VGNIANQIPSLNVIPYAPASTLFTDYAQKKRFIWMPNGETATYVNDYTILNFPVGTVL
                     LKTFYYTTIQPGNTTMLIETRVMVRKSDGWKFYEYLWNDAQTDASLVTTEDFANGSFK
                     SITFSKPNNDIVNTNYRIPSDGECLACHKINEVPTPIAVKPQNLNFNYDYTTGSMNQL
                     QKLVTQGYLDTYPSNIVSTVDYHDTSQPLGLRVRSYLDANCAHCHQDQGRCDYRNIRL
                     TFRQSSEDANIGICLTADEEVSPTLQKIISPGNYTKSIMHFRMSSNEESERMPLLGRT
                     IVHDEGVALLEQWINSLNQSCD"
     gene            7793..>9756
                     /locus_tag="PK218_08730"
     CDS             7793..>9756
                     /locus_tag="PK218_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPW98632.1"
                     /translation="MKKTVLLLILFFSTSMIVAQSDCASAIAVALNSTTTAPPFTNEN
                     GTVPPLLCGLTNGNNGVPTKGKWYKYTPTENLNITVSTVLPQNNNPDTRLFIYSGTCN
                     SLVCVGANDDFPGGVTSKVSFSATSGVTYYIAFDNRYSQSGFDFTVMESAPPPVDRLS
                     FTSTPVAGIAGTYNNCIVDMNGDYLDDIVSAVNTNQLVISYQLANGNFATSTFPLINT
                     VVSPSWSIAAGDFDNNGFNDLMYGSGSGVTFLKANNDGSAYTTDRKTQSFLVQRTNFI
                     DINNDGLLDAFACDDNAPNRLYINDGVNLNHTQGGIGDFASGGNYATNWFDFDNDGDL
                     DVYIAKCGQGGSGVGGNIDQLYRNNGDGTYTNIAAEANMANPEQTWSGAVGDFNNDGW
                     MDVIVGVNSLSDGHTNVKRNNGDGTFTSVTTGSGYDLVSSTGREYIAQDFDNDGFLDV
                     LGAGSTIMFGDGNFHFTPNPNTYPLSAVDRPVGDLNNDGFLDIQNGNNVLFNNGNSNN
                     WINVNLRGIQSNRNGIGARVEIYGAWGKQIRDVQSGTGFQNMSTITAHFGIGQETEIT
                     QIIIKWPSGTVDTIQNPSSNQSLLVVEGSTLAIADFNSATFSLYPNPAKKILNINSKN
                     GVAFKSVTIFDLSGRIVLKSAIENATVNVQT"
BASE COUNT         3404 a         1528 c         1705 g         3119 t
ORIGIN      
        1 ctaaaaccta tacttatatt ctaaaaaaag aaaacggtgt tgtacctgat agtttaaata
       61 cacacacaac cattactgtt cctataaaaa agattgtcgt tacttctaca acacatattc
      121 catcgttgga aatgcttgaa gtagaaaaaa ctctagttgg ttttccaaac ttagattata
      181 tttcttccga aaaagtgcgt gccttaattg atgaaaagaa aataaaagaa ttaggagcta
      241 atcaagcact aaacattgaa gtgctgattg atttgcaacc cgatgttatc attggatacg
      301 gcatcgacaa cgatacaaaa tcattagaga atttagaaaa tagcggttta aaagttttat
      361 taaatggcga ttggaatgaa gaaacaccat tgggaaaagc tgaatggatt aaacttttcg
      421 gtgccttgta tgacaaacaa gataaagcga aaaaactatt ttcaataatc gaaaaagact
      481 atctaaacac gattgaaata gccaagttag atagtgttac tccaactatt ttggctggtg
      541 atatgtttca agacaaatgg tatttaccaa aaggaaccag ttggggaagt attttgttga
      601 aagaagcaaa tgctaattat ttatgggcag atacttcggg aactggaagc ttgtcacttt
      661 cttttgaaac agtatatgaa aaagcaaaag aggctgattt ttggataact tctggtcagt
      721 tctcaacttt aaaagagatg atggatgcca atccgcatta tgagcaattt gaagccttca
      781 aaaaaggaaa tgtctattcc tttgctggaa aaaaaggaaa aacaggcggt gttctctatt
      841 atgaactagc acccaataga cccgatatgg tattgaaaga tttagtaaag attttgcatc
      901 ctgacttatt accaggttat gaaccttttt tctttgaaaa gattaagtag tgagaagctg
      961 gaagaaagaa gttagagtta tatgagtaaa aaccgaaata cattattatt tacttctttg
     1021 tcattggtgc tgctattgac cttagtcttg aatatttcta tcggtcaagt aaccattccg
     1081 tttaaagaag tatgtaaaag tctttttggc tcttcggcaa gcaaagagac ttgggaatat
     1141 atcatcatca attatcgttt acccaaagcc attacggcaa tgttggttgg tgtttcgctt
     1201 tctatcagtg gtttgttaat gcaaacattt ttcagaaatc cattggctgg accatttgtt
     1261 ttagggttga gttcaggctc tagtttaggg gttgcttttg tggttttagg aagtagtttt
     1321 ttgccaatct tcttaacgga ttttctgctt tcatcacatg gaatcatatt ggcttcttgc
     1381 gccgggagtt ttctggtatt actgctcatt tttattgttt ccaaatattt gcgtgatgta
     1441 atgtccatct taattgttgg gttaatgttt agtagtttta cttcagccat tgttagtatt
     1501 ttaacttatt ttagtactgc agcacaattg caaaagttca ccttttggtc aatgggaagt
     1561 ttaggtaatt tgacttgggg gaatatttca tttttaacgt ttgttattac tattggtttg
     1621 cttataagtg ttttttctct gaaatcatta gatgcattat tactcggaga aaactatgct
     1681 aaaagcatgg gattgaattt taagagattt aaaaacacca tcatcattag caccagttta
     1741 ttaacgggaa gcataaccgc ttttgttggt ccgattgctt ttattggttt ggcagttccg
     1801 catttaacaa aactactatt tcaaaccagt aatcataaaa tattatttat aggaacactt
     1861 ttaattggtg caataattat gttactttgt gatagtattt cgcaattccc tggaatggat
     1921 tttacactac caatcaatgc tataacttcg ctagttggag caccgattgt tatttggttg
     1981 ataattaaaa agaaaaatat tttatagata tgaaaacaat ttcacgaaac ctgatattac
     2041 ttttagtgct tgtaagtttt aacagctgta gcgaattatt ggattgtatt gtaagaactg
     2101 aacctgtttt acctaataaa actttagaaa ttgcaactgt tggtaacaat tacaatcaat
     2161 atattatagc cgaagttaaa aacgactcta atgatgatgc ttatttttat cattttagta
     2221 tcaatggaga tttgcccgct ggaataaatt acaacattag cgacagaaga ataagctttt
     2281 atggcgttcc aaccaatcct ggaacttttc gttttactgt tctactaacc attgattatc
     2341 ctaatgattc atattatgaa gataacgatg gtatttttat tgacgacaat cgaatttgtt
     2401 ttggtgataa aagaacatct aaaacgtatg aaattaaagt tcaatgagtg taaattcaaa
     2461 tgaaatttta attactaaac aattagcagt tggctattct tccaaaaaga gaaccaatgt
     2521 cattgcgaat agcattaatt tgaccttaca aaaaggaaaa ctcatcacct tagttggcgc
     2581 taatggtatt ggtaaatcta ctttattacg aactttaacc ggaattcaaa aaccactttc
     2641 gggcaacgtt tttctggaag ataaaaacat tcaatcttta gatgatttga ctttagccaa
     2701 aaacttaagc ttggttttaa ccgaaaaatt acctccaagt aatttgactg tttttgaatt
     2761 ggtggcactt ggaagacaac cttataccaa ttggttagga aaactttcag aaaatgactt
     2821 gaagattata aatgaagtta ttgccattac gcaaattgaa gagttactcg aaaaaaagca
     2881 ttttgaagtc agtgatggtc agttgcaaaa agtaatgatt gctcgtgctt tggcacaaga
     2941 tacgtcaata attattttag acgaaccgac tacacatttg gatttattgc acaaagtatc
     3001 ggtatttaaa ctcttgaaaa aactgtcaca agaaaccaat aaatgcatat tattttcaac
     3061 acacgatatt gatttagcaa tacaacttag cgacgaaatg attgtgatga cgcaagaaaa
     3121 agtgtatcaa gaccaaccat gtaatttgat ttcaaatgga gtttttgaaa ctttatttaa
     3181 agatgaaaaa atttcgtttg atggaacaaa agggaagttt gttatttcaa attaatttgt
     3241 cttttggctt caccaacaac aaaagccact gaattagcaa tattataact tctaatcaaa
     3301 ttagacatag gaatctttaa atgattttca aattggtcaa gcacttcttc tgataaacca
     3361 atgctttctt taccaaaaac caaccaatcg ccatcttgaa attcggcttc ataaatagat
     3421 ttatcaacac gtgaactcat caaaaacact cttgatttgt ctttaatttg agcaatccat
     3481 tcatctacat tttgatattc agtcaaatct aaatgcaccc aataatccaa acctgaacgc
     3541 tttaaattag tatcggttat atgaaagcct agtggatgaa ttaagtgtaa acgactttcg
     3601 gtgccaacgc aaagacgacc aatgtttcca gtattattag ggatttcggg ttgtactaaa
     3661 acgatgttta acatttgatt attcggttat ttgaaaaact agccacttct agaacttcac
     3721 cagctttaag ttgcaaatgt attgttccta ctcgaattct taccaatcgt aaagtaggaa
     3781 atccaacagc ggcagtcatt tttctaactt gacgaaattt gccttcggtt aaagtaatcg
     3841 aaacccaaga agtcggtccg tgacgttcat cgcgaattcg tcttccttcg ccaatacaat
     3901 caggaagttg gttgatgatt tttgcttcgc attttttggt tgtatatcga atgcctttaa
     3961 aaccaatttc tactccattt ttcaattgtt cgatagcttt attcgtaata acaccatcta
     4021 cttgagcgta atattctttt tcaaccgttt tgcttctaac gatttcactc atcattccgt
     4081 cggttgtcaa caataacaaa ccttccgagt cttcatctaa gcgaccaatt gccattgttc
     4141 cttcaggaaa agaatacaat tcaccaagca actttttatg tcgctttttt tcataaataa
     4201 attgactcaa atagccgtgt ggtttgtgaa ataggaaatg gcgatgcatg aataattatt
     4261 tgcaacaaaa atacaatttt gaaatcctac atttcaattt aatgtttaaa tttacaaaaa
     4321 tcataatttt agtatgtcag attcattatt catcattatt gctttcatta ttgcgcttgc
     4381 tattggaatt tatttaggaa aaatgctttt ttctgccaat tctaaatcag aaaaagcatc
     4441 attagaagaa aaaacaaacg gtttattatc gcaaatcaat cagttgaaag agcagtttca
     4501 aaatgaacgt aaccaatttg aaaagcaatt gtttgcatta aatgcagaaa aagaaactat
     4561 ccgcagtgaa aaagaagcct ttgctattca attgtctaaa aaagaaactg attttgaaaa
     4621 tctttgggaa cgaaataaag aacaaaaaga agaagtagaa aaattgcagg agaaattcac
     4681 caaagaattt gaaaatttag ccaataaaat actagaagaa aaaacttcaa agtttaccga
     4741 acaaaacaaa gaaaacctca agaatatttt atcgccattg caagataaaa ttcaactttt
     4801 tgaaaagaaa gtagaagaca cgcacaaaga aagtattgat tatcacgctg ctttacgtca
     4861 acaaatactt ggtttgcgtg aaatgaacga acaaatgagc aaagaaacgt tgaatttaac
     4921 caaagcattg aaaggcgaca gcaaaatgca aggaaattgg ggcgaattga ttttggaacg
     4981 cgttttagaa aaatcaggat tagaaaaagg tcgtgaatac gaagtgcaac agagttttac
     5041 taccgaagat ggaagccgag tttttcctga tgttgtcatc aatcttccgg atggaaagaa
     5101 aatggtggtc gattcaaaag tgtctttaac cgcttatgaa aaatatgtaa acgaaagtga
     5161 tgatgatgaa aaaggaacac atttaaaaga acatatcaac tccataaaac gtcacgtaga
     5221 acaattaggt aacaaaaact accatgattt atatcaaatg gaaagtcctg attttgtatt
     5281 gctttttata ccaatagaat cggcttttgc attagcattg aatgaagaca attctttata
     5341 caataaagct tttgaaaaga atattgtaat cgtcacacct tctacacttt tagcaacttt
     5401 gcgaactatt gatagcatgt ggacgaatca aaaacaacaa gaaaatgctt tggagattgc
     5461 acgtcaagca ggagcattgt atgacaagtt tgaaggtttt gttagcgatt tggtgaaaat
     5521 tggaaaaaaa atggacgaag ccaaaactga atatcaaggc gcaatgaaca aacttgtaga
     5581 tggaaaagga aacttgataa cgagtgttga aaaattgaag aaaatgggtg ccaaagcgaa
     5641 aaaagcatta cctgaaaaca tactaattag agccgaaaaa gatgaacaag aactttaaaa
     5701 ctgttgcaga atcgcaagta actctatcga tgttgatgtt gccttcccat tccaacttta
     5761 gtggaaaaat tcatggaggt tttatattgt cgcttttaga tcaaatagct tttgcaagtg
     5821 cttcaaaatt ttcgggcaat tattgcgtca ctgcttcggt agatactgtt gactttttag
     5881 acccaattga agttggagaa ttagtctctc taaaagcaag tgtcaatttt gttggaagaa
     5941 catcaatgat tgtaggcatt cgtgtagaag ctgaaaatat tcaaacagga aaagtaaagc
     6001 attgtaattc aagttatttt accatggttg ccaaagatga tgccggtaaa ccaacccaaa
     6061 ttccaggatt acttattaaa tcaaaacaag aatacagacg ttttttaaac gctttaaacc
     6121 gcaagaaaaa gcaaaaagaa atagatgatt acaaagtacc gattgattat aactcagaac
     6181 atctgaaaaa agaattaagt caatacaata ttagaattga aaattttatt tgattcagta
     6241 tttcgtaagt ttttaagtat aaatttgtca tattatgcaa ctaatgtttg atttttgatt
     6301 tttttgttta tatttggaat actttttaaa aaatacaatg aagaaaattt actgtctatt
     6361 tattgccttg tcagctctta ttattacttt ctatgcttgt tcagacgata acaattacac
     6421 tccaatcaat acttatccaa ttgcaactga tgatacggtt tcaaataatt taataaatcc
     6481 agttgtaata gatgtattat taaatgatac tactggagat gttcctgttg cttcaactgt
     6541 ttctataatt ggaggaacag acaccaattc aaacggaact ttagataatt tagtagttcc
     6601 aagtgaaggg acttggtctg ttaatgcaac ttctggaatg ataactttta caccagtacc
     6661 aaactatgtt ggaaacccat ccccaatttc atataccgta aaaaacgctc aaggaaatgc
     6721 ttccaacgaa gctacagtaa ctataaatgc tacaccaatt gtaagtgcag atatagaaaa
     6781 tattccatac gccaatttat cggagtatca tttttttgtt ggaaatatcg ccaatcaaat
     6841 tccttcatta aatgtaattc cttatgcacc tgcgagtact ttgtttactg attatgctca
     6901 gaaaaaaaga tttatttgga tgcctaatgg agaaacggct acttatgtaa acgattatac
     6961 catattaaat tttccagttg gaactgtctt gcttaaaacg ttttattata ctactattca
     7021 gccaggaaat acaaccatgt tgattgaaac cagagtaatg gttagaaaaa gcgatggttg
     7081 gaaattttat gaatatttat ggaacgatgc acaaacagat gcaagcttag taacaactga
     7141 ggattttgca aatggaagtt ttaaatccat tactttttca aaaccgaaca acgacatagt
     7201 aaatacaaat tatagaattc catcagatgg tgagtgctta gcgtgtcaca aaattaatga
     7261 agtgcctacg ccaattgctg taaaacctca aaatcttaat tttaattacg actatactac
     7321 aggaagtatg aatcaattgc aaaaactagt tacccaaggt tatcttgata cctatccttc
     7381 aaatattgtt tcaacggtcg attatcatga cacttcacaa ccattaggtt tgagagtacg
     7441 ttcttattta gatgctaatt gtgcacattg tcatcaagat caaggacgat gtgattatag
     7501 aaacatccgt ttaacattta gacaatcaag tgaagatgca aacataggaa tttgtttaac
     7561 cgctgatgaa gaagtaagcc caacattaca aaaaataatt tctccaggaa actataccaa
     7621 atcaattatg cattttagaa tgagttctaa cgaagaaagt gaaagaatgc ctttgttagg
     7681 aagaactatt gtgcatgatg aaggtgtagc acttttggaa caatggatta actccttaaa
     7741 tcaatcttgt gattaattta gatactatca atacaatatt aaaaaccaat ttatgaaaaa
     7801 aacagtactt cttttaattt tattcttcag cacttcaatg atagtggcac aatcggattg
     7861 tgccagcgca atagctgttg ctttaaactc aactacaact gcgcctccat ttaccaatga
     7921 aaatggaact gtgccacctc ttttgtgtgg attgacaaat gggaataatg gtgttcccac
     7981 aaaagggaaa tggtataaat atactccaac cgaaaattta aacattactg tttcaactgt
     8041 actacctcaa aataacaatc ccgatactag attatttata tattccggaa cgtgcaacag
     8101 tttagtttgt gttggcgcca acgatgattt tcctggcgga gtaacatcaa aagtatcttt
     8161 ttctgcaacc agtggagtta catattatat agcatttgat aacagatata gccaaagtgg
     8221 ttttgatttt acagtaatgg aatctgctcc accacccgtg gacagacttt cctttacttc
     8281 aacaccagtg gcaggaattg caggaacata taacaattgt atagtagata tgaatggtga
     8341 ttatcttgat gatatagtat cagcagttaa tacaaaccaa cttgtcattt cataccaact
     8401 agccaacgga aactttgcaa catctacttt tccattaatt aatacagttg tttctccatc
     8461 ttggagtata gcagcaggcg attttgataa taatggtttt aatgatttaa tgtatggttc
     8521 gggtagtggc gttaccttct taaaagcaaa taacgatggc tcagcataca caaccgatag
     8581 aaaaacacaa tcttttttag tgcaaagaac aaattttata gacattaata atgatggttt
     8641 gttagatgct tttgcatgtg acgataatgc tccaaaccga ttatacataa atgatggtgt
     8701 aaatttaaat catacacaag gtggtattgg tgattttgca tcgggtggaa attatgcaac
     8761 caattggttt gattttgata atgatggaga tttagatgtt tacattgcaa aatgtggtca
     8821 aggtggttct ggagtaggag gaaatattga ccaattgtat agaaataatg gtgacggaac
     8881 atataccaac attgctgctg aagcaaatat ggcaaaccct gaacaaacat ggtctggtgc
     8941 tgttggcgat ttcaataatg atggatggat ggacgttata gtcggagtaa attcattatc
     9001 cgatggacat acaaatgtta aaagaaataa tggtgatggt acgtttacta gtgttacaac
     9061 tggttctgga tacgatcttg tttcttcaac gggaagagaa tacattgcac aagattttga
     9121 caatgatggt tttttggatg tattaggtgc tggaagtaca atcatgtttg gtgatggaaa
     9181 ttttcatttt acaccaaacc caaacaccta tccattatca gcagttgata gacctgttgg
     9241 cgacttgaat aatgatgggt ttttagatat tcaaaatggt aataatgttc tttttaacaa
     9301 tggaaattct aataattgga taaatgtaaa cttgagaggt attcaaagta atagaaacgg
     9361 aattggcgca agagttgaaa tttatggtgc atggggaaaa caaattagag atgttcaaag
     9421 tggtactggt tttcaaaaca tgagcacaat aaccgctcat tttggaattg gtcaagaaac
     9481 agaaattact caaataatta taaaatggcc gtcgggaact gttgatacca ttcaaaaccc
     9541 ttctagcaac cagtcgttat tagtagttga aggttctaca ctagcaatag ccgattttaa
     9601 tagtgctacc tttagtttat acccaaaccc agctaagaaa attttaaata taaattctaa
     9661 aaatggcgtt gcttttaaat cagtaacaat atttgattta agtggaagaa ttgttcttaa
     9721 atctgctatc gaaaatgcaa ctgttaatgt tcaaac
//
LOCUS       DAOOBH010000086         9740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_8698_length_9740_cov_5.596902, whole genome shotgun sequence.
ACCESSION   DAOOBH010000086 DAOOBH010000000
VERSION     DAOOBH010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9740
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8698_length_9740_cov_5.596902"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PK218_08735"
     CDS             <1..376
                     /locus_tag="PK218_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW98633.1"
                     /translation="IVNTSVFILPLGNDLFKVGATYNWKDKTVFPTEEGKKELVERIK
                     EILSCEFQIIQHFAGVRPTVNDRRPLLGSHPKHKNLYILNGLGTRGVMLAPAMAIDLY
                     NYIENKIPLDKTIDINRYAHFL"
     gene            complement(351..746)
                     /locus_tag="PK218_08740"
     CDS             complement(351..746)
                     /locus_tag="PK218_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HPW98634.1"
                     /translation="MLKKGSKLYSILTGSCPRCHEENMYVDKNPYHLSKLYQMHETCS
                     HCKLQYKIEPSFFYGAMYVSYGLTVAIGVATFIVAKVFIGLDLFFSFMAIIAALILTT
                     PITFRLARNIYINIFVHYNPEATKNVHND"
     gene            813..2717
                     /locus_tag="PK218_08745"
     CDS             813..2717
                     /locus_tag="PK218_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPW98635.1"
                     /translation="MLNIHNLSVSFGGTFLFEEVTFRLGSGDRVGLVGKNGAGKSTML
                     KILAGDFKPDSGQIATEKEVRMGFLRQDIDFEVGRTVLEEAYEAFTEIKRVERKLEEI
                     NHQLVTRTDYESEDYSQIIEQLSDYTHQFEILGGYNYVGDTEKILLGLGFRREDFNNQ
                     TDTFSGGWRMRIELAKLLLQSNDILLLDEPTNHLDIESIIWLESFLRNFPGVVVIVSH
                     DKMFLDNVTNRTIEISLGKAYDFNKPYSQYLVLREEIREKQLATQKNQAKKIEETEKL
                     IEKFRAKASKASMAQSLIKKLDKVERIEVDEDDNSVMNISFPVSQTPGRVVIEAEHVT
                     KAYGDKVILKDISLLVERGSKIAFVGQNGQGKSTFIKAIVNEFKYDGTIKLGHNVQLG
                     YFAQNQAEYLDGEITLLETMINAATDTNRSKVRDMLGSFLFRGDDVEKKVKVLSGGER
                     NRLALCKLLLQPINVLLMDEPTNHLDIKSKNVLKAALNKFEGTLLLVSHDRDFLQGMA
                     NTVYEFKDQKIREYLGDINFFLEQRNAMNMREIEKKDVVKESPKVEKKNLSFEEQKAQ
                     KSLQNRLSKVESQIQQLEKDIQHDDRELASNYDKHIENASFFAAYEKKKVELEKLLAE
                     WEEIQLEIES"
     gene            complement(2721..3869)
                     /locus_tag="PK218_08750"
     CDS             complement(2721..3869)
                     /locus_tag="PK218_08750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW98636.1"
                     /translation="MNQDITFSIFKTVNELPKEWDIVSQSNAFLQTPYLHVLEQSAPT
                     NMECFYIGIFEHQQLIGVALAQYLDLNKLESFGERDKCLKTYIRNFIFKNFASHALFL
                     GNNMITGQNGFVFSKPMEFNCISEILLNCSQTLIAYFKTKKIKIHIVSFKDFYKKSAT
                     ELQHYGFATIYEFNTQPNMIFELDKNWKTEADYVSAFSKKYRDQYKRCRKKSEGIVTR
                     ELTTQEIIEQEKRIYELYHHVAKNAPFNTFFLAEDHFTVFKKQCGNRFVMVGYFLENE
                     LIGFHSLLLNGEVLETYFLGYDEQIQKEKMLYLNMLYNMTAFGIEHQFKKIIFGRTAL
                     EIKSSIGAEPIMMSGFIFHTNSFINKFMPKIFPKLEPTLAWQQRHPFK"
     gene            3948..4235
                     /locus_tag="PK218_08755"
     CDS             3948..4235
                     /locus_tag="PK218_08755"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HPW98637.1"
                     /translation="MKKTMKPFSNSEIEEALKKVNGWNYTEAKIQKKYQFKDFSEALS
                     FLIRVGILAEKQNHHPEIWNVYNKVTLSLNTHDANGITEKDFILAESIDKL"
     gene            complement(4275..4754)
                     /locus_tag="PK218_08760"
     CDS             complement(4275..4754)
                     /locus_tag="PK218_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HPW98638.1"
                     /translation="MEMNFLAILVAALSTLIVGFVWYGPLFGKVWMKENGFSEEEMKK
                     GNMLKIFGLTIVFSLLFAMIMQVLVIHQFGALGMIGGDVTTAKQSYVDFMADYGNAYR
                     TYKHGALHGFMTGIFLAFPMIAINGLFERKSWKYIFIHSGYWTVCMIIIGAIVCGWV"
     gene            4959..5813
                     /locus_tag="PK218_08765"
     CDS             4959..5813
                     /locus_tag="PK218_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Two component regulator three Y domain protein"
                     /protein_id="HPW98639.1"
                     /translation="MKTIFTYISVMLVSVSVFAEISKTEKNALIKLYKSTNGAKWTNT
                     WDLKAAPTTWYGVEIKDDKVVSLKLMNNNLAGNLPNELGNLTSLVEINFFRNYITGIL
                     PATLGNLKSLTHLNVAFNQVSGSLPESLGNATQLQSIELHMNRLNGQLPTSIGTLSSL
                     ETLSLFNNEIEGEIPSSYYQLKALKVLLLSSNKLVGALSPDVANLTSLENLSLFENKM
                     DGQVPFDLEKLHNLKEMNISYNMFNGLVSRNLAKLDTLNMTMINEQGVATTLKISIDK
                     NSAYAADE"
     gene            complement(5911..6363)
                     /locus_tag="PK218_08770"
     CDS             complement(5911..6363)
                     /locus_tag="PK218_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HPW98640.1"
                     /translation="MNTKKIAEYRALLEVTKTATLKELKTIYRNCVKDCHPDKFTDEA
                     EKLAAEEKSLLIIEAYHFLVSIAPETLEKTKAAYTATTTTSAIEDFYFEKGVLYVTFL
                     DGSTYEYFGIPKNTYVKMINAESPSRFARRHIYNQFLYRSASKLVAAE"
     gene            6472..6813
                     /locus_tag="PK218_08775"
     CDS             6472..6813
                     /locus_tag="PK218_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98641.1"
                     /translation="MKKAFFFIAFSAMILLLQSCKKHSEPSTETNSVYTNNINKGLEK
                     HNLQQLDSAFYYYNQASQTAYDGDSKAYALFQIATIQQQVGDFTEAKKPLLTHSNKQN
                     QTFMHPIYTIF"
     gene            6870..8114
                     /locus_tag="PK218_08780"
     CDS             6870..8114
                     /locus_tag="PK218_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW98642.1"
                     /translation="MYNDTLNNLDKAIIKNNIAVVYLEKKEFQKAIQIEEALLTNTVL
                     QNDKKNYAKVLDNLGYAYFKTNNPKALPFLNQSKFLRDSLNNDYEKIATYIHLAEYHQ
                     ATNPKLALQMATQAFTFANATNNPDDKLEALKLKVALSNPTTTKEVALQHIQLSDSIF
                     KVRQFAKNQFAKIKYDASKAVLEKDKSENQKQIILWLLFAVAIIAINIILILRYRNKQ
                     KLKASIYDTEIRISKKIHDELANDVFQAMTYAETQDLQNNTKKEILLDSLENIYSLTR
                     DISQSNSQIDTKEHYKKQLFELLNNFSNASINVIIKSDNTINWDKLHKEIKITLHRVL
                     QELMVNMKKHSNCSIVVITFFNHPKHLEIKYADNGQGINPQTFDKKGLQNVENRIQAI
                     KGTIIFDNQTNKGFKVTISIPK"
     gene            8121..8789
                     /locus_tag="PK218_08785"
     CDS             8121..8789
                     /locus_tag="PK218_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW98643.1"
                     /translation="MYKKVLISEDIDTINLAIQLTLFQLAITDIQHVKYCDDALLRVK
                     KAIRDNEPFDLFITDLSFEPDKRSVKLTSGEQAIAEIRKTQPNIKVIVYSVEDKKHRI
                     KELYNNYNINAYVHKNRNSIEQLKTAITNTYQTETAYISPELKHLLANKTNFQIDEID
                     IQILKQIAFGYTYEEIANLFKQQNIIPSSKSAIEKKVNKLKDYFKANNTIHLITITKD
                     LGII"
     gene            8868..9110
                     /locus_tag="PK218_08790"
     CDS             8868..9110
                     /locus_tag="PK218_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98644.1"
                     /translation="MKLYYIDRYPNQDNNYELHIANCNLLPNIFNRACLGNFDTQEEA
                     LLEANKKYPKSILCTNCLKAEHNKAKPFFKRWFQKK"
     gene            9393..>9740
                     /locus_tag="PK218_08795"
     CDS             9393..>9740
                     /locus_tag="PK218_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98645.1"
                     /translation="MKQIYLNILLLLIYGFGNANNFVLPPSATISGTTTVCQNAASPT
                     ITFTGSGGTAPYTFTYTVSGTAGNQTILTTSGNSVTISAPTNVVGSFTYSLVSVKDGT
                     NTTQNQSGTAIITV"
BASE COUNT         3722 a         1783 c         1420 g         2815 t
ORIGIN      
        1 tattgtaaat acaagtgtat ttattttacc tctaggaaac gatttgttca aagtaggagc
       61 aacttataat tggaaagaca aaacagtttt tccaactgaa gaaggtaaga aggaacttgt
      121 tgaacgaata aaggaaattc tttcttgcga atttcaaatt attcagcatt ttgctggagt
      181 tcgtccaaca gttaatgata gaagaccatt acttggctca catccaaaac ataaaaacct
      241 atacatttta aacggacttg gaacacgtgg agtaatgctt gctccagcta tggcaatcga
      301 tttgtataat tatatcgaaa ataaaatccc tttagataaa actattgata ttaatcgtta
      361 tgcacatttt ttgtagcttc aggattgtag tgtacaaaaa tatttatata gatatttcga
      421 gctaatctaa aagtaattgg agttgttaag attaacgcag caataattgc catgaaagaa
      481 aaaaacaaat ctaatccaat aaagactttt gcaactataa aagtagctac gccgatggca
      541 acagtcaaac catagcttac atacattgca ccataaaaaa acgatggttc tattttgtat
      601 tgcaatttac aatgactgca agtctcgtgc atttgataca atttagataa atgatacgga
      661 tttttatcaa catacatgtt ttcttcatga catctcggac aacttcctgt taaaatacta
      721 tatagtttgg atcctttttt taacatttgc aaaaatttta tacaaaggta atcttattat
      781 aaaattatca atgtaacatt tgtaacaatt atatgctaaa tattcataat ttatcggttt
      841 cttttggtgg tacttttctt tttgaagaag taactttcag acttggttct ggcgacagag
      901 ttggacttgt tgggaaaaat ggtgcgggaa aatctaccat gctaaaaatt ttagcaggtg
      961 atttcaaacc cgactcagga caaattgcta ctgaaaaaga agtgcgtatg ggttttctac
     1021 gtcaagatat cgattttgaa gtcggtagaa ctgttcttga agaagcctat gaagcattta
     1081 cagaaataaa aagagtagag cgaaaactag aagaaataaa tcatcaactt gttacacgaa
     1141 ctgattatga aagtgaagac tattcacaaa tcatagaaca attatctgat tatacacatc
     1201 aatttgaaat tttaggcggt tataattatg ttggcgatac cgaaaaaatt cttcttggat
     1261 taggttttag acgtgaagat ttcaataatc aaaccgatac attttctggc ggatggagaa
     1321 tgcgtatcga attagctaaa ttgttattgc aatcaaatga tattttacta ctcgatgagc
     1381 caaccaacca cctagatatc gaaagtatta tttggcttga aagtttcctt agaaatttcc
     1441 ctggtgttgt ggtaatcgtt tctcacgata aaatgttttt ggataatgta accaaccgaa
     1501 ctattgaaat ttcattagga aaagcttacg atttcaataa gccttattcg caatatttag
     1561 tgttacgtga agaaattcgc gaaaagcaat tggctactca aaaaaatcaa gccaaaaaaa
     1621 ttgaagaaac cgaaaagcta attgaaaaat ttcgtgccaa agccagcaaa gcttccatgg
     1681 cgcaatcatt aattaaaaaa cttgacaaag tagaacgtat agaagtagat gaagacgata
     1741 attcggttat gaatatatcc tttccagttt ctcaaacgcc tggtcgtgtt gttatagaag
     1801 ccgagcatgt aaccaaagcg tatggtgata aagtcatttt aaaagacata tcacttttgg
     1861 ttgaacgcgg aagtaaaatt gcgtttgttg gtcaaaatgg tcaaggaaaa tcaacgttta
     1921 ttaaagccat tgttaacgaa tttaaatacg acggaacaat aaaactaggt cataatgttc
     1981 aacttgggta ttttgctcaa aatcaagcag aatatttaga tggcgaaatt actttattgg
     2041 aaactatgat taatgcggca acagatacca atcgttccaa agttcgcgac atgttgggtt
     2101 cattcttgtt tcgtggtgat gatgttgaaa aaaaagtaaa agtgctttct ggtggcgaaa
     2161 gaaaccgttt ggcattatgt aaattattac tgcagccaat aaacgtttta ttgatggatg
     2221 agccaaccaa ccacttagac atcaaatcta aaaatgtgtt gaaagcggcc ttaaataaat
     2281 ttgaaggaac tttattgttg gtttctcacg acagggattt tcttcaagga atggcaaaca
     2341 cggtttatga atttaaagat caaaaaataa gagaatatct tggcgatatc aatttcttcc
     2401 ttgagcaaag aaacgctatg aatatgcgcg aaattgaaaa gaaagatgtg gtaaaagaat
     2461 ctccaaaagt tgaaaagaaa aatctttcct tcgaagaaca aaaagcgcaa aaatcgcttc
     2521 aaaatcgttt aagtaaagtt gaaagtcaaa tccaacaatt ggaaaaagac attcagcacg
     2581 acgatagaga attagcgtca aattatgaca aacacatcga aaacgctagt ttttttgcag
     2641 cctatgaaaa gaaaaaagta gagttggaaa aactacttgc ggaatgggaa gaaattcaat
     2701 tagaaatcga aagttaatat ttatttaaac ggatgtcgtt gttgccaagc caatgttggt
     2761 tctaatttcg ggaagatttt aggcataaac ttgttgatga atgaattggt gtgaaaaata
     2821 aaccccgaca tcattatcgg ttccgcacca atagaacttt tgatttccaa cgctgttctt
     2881 ccgaagatta tttttttaaa ttgatgttca ataccaaaag cggtcatgtt atacaacata
     2941 ttcaaataca acattttttc tttttgtatt tgttcgtcat aaccaagaaa atacgtttcc
     3001 aaaacttcgc cattgagtaa aagcgaatga aaacctatca attcattttc taaaaaataa
     3061 ccgaccatta caaatcgatt gccacattgt tttttaaaaa cagtaaaatg atcttcggct
     3121 aagaaaaaag tattaaaagg tgcatttttg gcaacatgat gatacaattc ataaatccgt
     3181 ttttcctgtt caatgatttc ttgggttgtc aactctctag taacaatacc ttctgatttt
     3241 tttcggcaac gtttgtattg gtctctgtat ttttttgaaa atgcgctaac ataatcagct
     3301 tcagttttcc agtttttatc caattcaaaa atcatattgg gttgcgtgtt gaactcataa
     3361 attgtggcaa aaccataatg ctgcaattcg gtagccgatt ttttatagaa atctttaaac
     3421 gatacaatat gaattttaat ctttttagtt ttaaaataag caatcaatgt ttgagagcaa
     3481 tttaagagaa tttcactaat acaattaaat tccatcggct ttgaaaacac aaacccattt
     3541 tgacccgtaa tcatattatt tccaagaaac aaagcatgag aagcgaaatt cttaaatata
     3601 aaattccgta tgtaagtttt caaacattta tcgcgttcgc caaaagattc taatttgttt
     3661 aaatccaaat attgcgccaa tgcaacacca attaattgtt ggtgttcaaa gattccaata
     3721 taaaagcatt ccatattggt aggagcagac tgttctaaaa cgtgtaagta tggagtttgt
     3781 aaaaaagcat tcgattggga aacaatatcc cattcctttg gaagttcgtt taccgtttta
     3841 aatatagaga aagtaatgtc ttggttcaaa tcaactattt atgttttcaa aagtaattaa
     3901 ataatgcagt gttttagaaa cattatttta tatttaacta attttaaatg aagaaaacca
     3961 tgaaaccatt ttcaaattca gaaatagaag aagcattaaa aaaagtaaac ggttggaact
     4021 atactgaggc taaaattcaa aaaaagtacc aattcaaaga tttcagtgaa gcattaagtt
     4081 ttttaataag ggttggaatc ttagccgaaa aacaaaacca tcatcccgaa atttggaacg
     4141 tttataataa ggtaacactc tctttaaaca cacacgatgc aaacggaatt accgaaaaag
     4201 attttatact agcggaaagc atagataagc tataaaaaaa tcgctcttaa actaagagcg
     4261 attttcttta ttaattaaac ccatccacaa acgatggcac caattataat catacaaaca
     4321 gtccaataac cagaatgaat aaagatgtat ttccacgatt tgcgttcaaa aagtccgtta
     4381 atagcaatca ttggaaaagc caagaagatt ccagtcataa atccatgcaa tgcaccgtgc
     4441 ttgtatgttc tataagcatt gccataatca gccataaaat cgacatacga ttgttttgca
     4501 gtagttacat caccaccaat catgcctaat gcgccaaatt ggtgaataac taacacttgc
     4561 ataatcattg caaatagtaa ggaaaaaaca atcgtcaatc caaagatttt caacatattt
     4621 ccttttttca tttcttcttc agaaaatcca ttttccttca tccagacttt tccaaacaaa
     4681 gggccatacc atacaaagcc aactattaat gttgacaaag cagcaactaa aattgctaaa
     4741 aaattcattt ccatatttaa tatgtttaat tggttagtaa ttcaaatgta ttaaaaaaac
     4801 attttactac aaacaaataa taagctatcc ttttttatta aaatcataag aaaaatatag
     4861 ataaaaaata ttaaaaaatg tatttcatcg attttatttg tttttaaacg atgttctgta
     4921 tagttttaca ccgtcataac aaccaaatct tatatgatat gaaaactata tttacttaca
     4981 tttccgtaat gcttgtttcg gtttctgttt ttgcagaaat atccaaaaca gaaaaaaatg
     5041 cactgattaa gttgtacaaa tcaaccaatg gtgcaaaatg gacaaacact tgggatttaa
     5101 aagcagcacc aactacatgg tatggcgttg aaattaaaga cgacaaagtg gtttcattaa
     5161 aattaatgaa taacaatcta gcaggaaatt tgcctaacga attagggaat ttaaccagtc
     5221 tagtggaaat caattttttc cgcaactaca ttactggtat tttgccagct actttaggaa
     5281 atctaaaaag cctaacgcat ttgaatgtgg cattcaatca agtatcaggt agcttgccag
     5341 aatcacttgg aaatgctaca cagcttcaat caatcgaatt gcacatgaat cgtttaaatg
     5401 gtcaattacc aacaagcatt ggaactttaa gcagtttaga aacattgtct ttattcaata
     5461 acgaaatcga aggcgaaatt ccatcaagct actatcaatt aaaagcatta aaagtacttt
     5521 tattaagcag caacaaatta gtgggtgctc taagtcccga tgtagcaaat cttacatcat
     5581 tagaaaacct tagtttgttt gaaaacaaaa tggatggtca agtgccattt gatttagaga
     5641 aattgcacaa cttaaaagaa atgaatatct cttataacat gttcaacggg ttagtatcaa
     5701 gaaatttagc caaattagat acgctaaaca tgaccatgat aaatgagcaa ggtgtagcaa
     5761 ctacactaaa aatcagcatc gataaaaact ctgcctacgc tgcagacgaa taaaaatacg
     5821 ttcttatgat tgtaattaat tttgttgggt taacaaaatg taaaaagtct cgagcaatcg
     5881 agactttttt atgtttacta cgttcagtaa ttattcagca gctaccaact ttgaagcact
     5941 tctatacaaa aactgattat aaatatgacg tctagcaaaa cgacttggcg attctgcatt
     6001 aatcattttt acataagtgt ttttaggaat tccaaaatac tcataagtac taccatccaa
     6061 aaaagtaaca tacaacacac ctttttcaaa ataaaaatct tcaatggccg aagttgtagt
     6121 agttgcagta taagccgctt tagttttctc caaagtctca ggagcaatac ttacaagaaa
     6181 gtgatacgct tctataatca ataaactttt ttcttcagca gccagttttt cagcttcatc
     6241 cgtaaattta tcaggatgac aatccttaac acaattgcga taaatcgttt ttaattcttt
     6301 caaagtagcc gttttagtta cttccaataa tgcgcggtat tctgcaattt tctttgtatt
     6361 catctttgta atgctttgcc tttcagcttt tttaaatttt cggcaaaggt aaactttctt
     6421 tttacataaa ataataaatc acaatccaat attaattaat aatttaggac tatgaaaaaa
     6481 gcattttttt ttatagcctt ttcagcaatg atattgctct tgcaatcttg taaaaaacat
     6541 tcagaaccaa gtactgagac caatagcgta tatactaaca acataaataa aggccttgaa
     6601 aaacacaacc ttcagcaact agacagtgct ttttactatt acaatcaagc aagccaaacg
     6661 gcttatgatg gcgattcaaa agcctatgcg ttatttcaaa tagcaaccat tcaacaacaa
     6721 gtaggcgact ttacggaagc gaagaaaccg ctattgacgc actcaaacaa gcaaaatcaa
     6781 acctttatgc accctattta tacaatcttt taggcattgc atttctagaa caaaacaact
     6841 tcagcgaagc aattcaatac tataataagg tgtacaacga cactttaaac aatctcgata
     6901 aagcaatcat caaaaacaac attgccgtag tgtatctcga aaaaaaggaa ttccaaaaag
     6961 caatccaaat agaagaagcg ctactcacaa acacggtatt acaaaacgac aaaaaaaact
     7021 acgccaaagt actcgataac ctaggctatg cctacttcaa aaccaacaac cccaaagcac
     7081 taccgttttt aaaccaatcc aaattccttc gcgattcact aaacaacgat tacgaaaaga
     7141 ttgccaccta tatccatcta gccgaatacc accaagcaac aaaccctaaa cttgccctgc
     7201 aaatggcaac ccaagcgttc acgtttgcca atgccacaaa caatcccgat gacaaactag
     7261 aagccttaaa actaaaagta gcactcagca atccaacaac aacaaaagaa gtagcacttc
     7321 aacacattca actttcagac agtattttca aagtaagaca atttgccaaa aaccaattcg
     7381 ccaaaatcaa atacgacgca tccaaagcag tgttagaaaa agataaatcc gaaaaccaaa
     7441 aacaaattat tttgtggcta ctattcgcag tcgccatcat cgccatcaac attatcctaa
     7501 tcctgcgcta tcgaaacaaa caaaaactaa aagcatccat ctacgacacc gaaatcagaa
     7561 tctccaaaaa aattcacgac gaactagcca acgacgtctt ccaagccatg acctatgccg
     7621 aaactcaaga cctccaaaac aacaccaaaa aagaaatctt gctagattca ttagaaaata
     7681 tctattcgct aacaagagat atttcgcaat ccaatagcca aattgatact aaagaacact
     7741 ataaaaaaca attatttgag ttactcaata attttagcaa tgcatcaatc aatgttatta
     7801 taaaatctga taacaccatc aattgggaca aacttcataa agaaatcaaa ataacacttc
     7861 atcgagtttt gcaagaactc atggtcaaca tgaaaaaaca ttccaactgc tccatcgtgg
     7921 taattacctt cttcaaccat ccaaaacacc tcgaaataaa atatgccgac aatggtcaag
     7981 gaataaatcc acaaacattt gataaaaaag gacttcaaaa tgtggaaaac cgtattcaag
     8041 ccataaaagg aaccattatt tttgacaacc aaaccaacaa aggcttcaaa gtaactattt
     8101 ccatcccaaa ataaggcacc atgtataaaa aagtcctaat ttccgaagac atcgacacca
     8161 tcaacctagc cattcagcta accctttttc aattggcaat aaccgacata caacacgtca
     8221 aatactgcga cgacgcacta ctcagagtaa aaaaagcaat ccgagacaac gaaccattcg
     8281 accttttcat caccgaccta tccttcgaac ccgacaaacg atcagtaaaa ctaacatcag
     8341 gagaacaagc tatagccgaa atccgcaaaa cacaacccaa catcaaggta attgtatatt
     8401 ccgtagaaga taaaaagcac cgcatcaaag aactctacaa caactacaac atcaatgctt
     8461 atgtgcacaa aaaccgcaac agtatagaac aacttaaaac cgccattaca aatacctatc
     8521 aaacagaaac cgcttatatt tccccagaac tcaaacacct cctagcgaat aaaaccaact
     8581 tccaaattga tgaaatagac attcaaattc taaaacaaat agcattcgga tacacctacg
     8641 aagaaattgc aaaccttttt aaacaacaaa atataatacc aagtagcaaa agcgccattg
     8701 aaaaaaaagt aaacaaactc aaagactatt tcaaagccaa caataccata catttaataa
     8761 ccattacaaa agatttaggt attatctaaa aaaaaaatcc acattacggt tttccgtaaa
     8821 atacatccaa aataccttca tacgtttgtt gcaacaaaga caaacaaatg aaactctact
     8881 atatagaccg atacccaaac caagacaaca actacgaatt gcacatagca aactgcaatc
     8941 tgctacccaa cattttcaac agagcatgcc ttggcaattt tgacacccaa gaagaagcac
     9001 ttttagaagc caataaaaaa tacccaaaat ccatcctctg cacaaattgc ctaaaagcag
     9061 aacataataa agcaaaacca ttcttcaaaa gatggttcca aaaaaaataa ttgtggaagt
     9121 gtctgccctg gagcgaataa gtaatgccaa ttcctccagt ggcactcact tatattttta
     9181 ctcctctaaa gtaaaaaata agctgatttt tcataatcaa tttaatcaac taatattcaa
     9241 caaacatcaa actttttaaa aacgattgta gtaaaataac aaaaatagtt ttacaatgtt
     9301 ttttgtatta aaaaaactat tatttttata aacttaataa gataaatagt aaactaaata
     9361 aaattagtgt aaattaaaat tctcatttaa ttatgaaaca aatatactta aacatcttac
     9421 tgctattaat ttatggattt ggtaatgcaa ataattttgt tttaccacca tcagcaacaa
     9481 tttcaggaac tacaacagtc tgtcaaaatg cggcaagtcc tacaataacc tttacgggct
     9541 caggcggaac agctccttat acttttacat atacagtttc aggtacagca ggaaaccaaa
     9601 caattttaac aacatcagga aatagtgtga ccatttctgc accaactaac gtagttggtt
     9661 cttttactta tagtctagtt agtgttaaag atggtactaa tactacacaa aatcaatcag
     9721 gaacagcaat aataacagtt
//
LOCUS       DAOOBH010000087         9590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_8874_length_9590_cov_5.890718, whole genome shotgun sequence.
ACCESSION   DAOOBH010000087 DAOOBH010000000
VERSION     DAOOBH010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8874_length_9590_cov_5.890718"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..721)
                     /gene="nth"
                     /locus_tag="PK218_08800"
     CDS             complement(68..721)
                     /gene="nth"
                     /locus_tag="PK218_08800"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPW98646.1"
                     /translation="MVSNEEQDRWASNLHPLINLYHGKKHPLEYNNLYQLMIMVILSA
                     QDSDANINKISPILFKTFPNIESIAISTPEALLPYISKVRNFETKASWIIQIAQKLKE
                     DKNIPTNLSDLIALKGIGRKSANVILREINKPAEGIIADLHVIRVAPRIGIISEVKDG
                     NKVEKQLMEKLPKSIWGEIGMSISFLGREICRPTNPKCDICVVNKCCNYFKSNGKNL"
     gene            complement(725..1588)
                     /locus_tag="PK218_08805"
     CDS             complement(725..1588)
                     /locus_tag="PK218_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW98647.1"
                     /translation="MIKKEDKTSHSLQIPKGILYTSRFLEAISTKLTTLFAAKLFATP
                     IKHKIPKREWNMNRNSIQSTIKIPKIGKEIVIYEYGKSSKKVLLVHGWSGRGTQLYKI
                     ADALIENGYSTISFDAPAHGKSKGNKTLMPEFMACILEIEKHFAPFEFAIGHSLGGMA
                     ILNALKQNFTINKAIIIGSGDVIQDIIDDFVKKIGLNHKIGIKLKEYYEKKYGEPMED
                     YGSSFVSKDISQPILIIHDKNDDDVDVKAAYQINKSLKNSQLMITEGLGHRKILGDKI
                     VIENSIKFLKE"
     gene            1665..3596
                     /locus_tag="PK218_08810"
     CDS             1665..3596
                     /locus_tag="PK218_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HPW98648.1"
                     /translation="MQNQTRIVIENVNPQLDSGAFFIKRIIGQKVIVTANVFSDGHDV
                     IASSVKYKHEKEKKWQEIRMQETGNDEWIAQFKVEKQGFYTYFVEGWVDYALNWQHGT
                     ERKINDNQHVKSELLEGAEYCQAILKEVSKDEKAFLNNAIKAFQDEKKYNEAIAIALS
                     KELHDIFYNHPTRTLANSSIELKVYVDRKKALFSTWYEFFPRSSSEVKGKHGTFKDCE
                     KLLPRVASMGFDTLYFPPIHPIGEVNRKGKNNATNAQPGDVGSPWGIGSKHGGHKSTH
                     PELGTLEDFKSLVKKAKELGIEVAMDYALQAAPDHPYVKDFPQWFKWRPDGTVQYAEN
                     PPKKYQDIQPIYFESGDWKNLWKELLDVALYWVEECDIKVFRVDNPHTKPFYFWGWLI
                     AEVKKKHPDVLFLAEAFTRPKIMNELAKQGFTQSYTYYTWRNSKAELIEYLEELTKSE
                     QKEYFRPNFWPDILPYALQSGMESVYLHKYFLAATLSSSVGLYGPVYEYMISDAMPGK
                     EEYLNSEKYEVYHWDWTIQNKLVTVISRLNRIRHEQPSLQQTNNIEFCATDNDQVIAY
                     YKYDDDKLDETLMICNIDPYYPKQAWVQLPLKSLKVQAGQPIKVVDLVTGNSYIWDKE
                     WNFVELHPSLPFHLFKIIK"
     gene            3624..5246
                     /locus_tag="PK218_08815"
     CDS             3624..5246
                     /locus_tag="PK218_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose synthase"
                     /protein_id="HPW98649.1"
                     /translation="MQTSSEETFTSPFEFKTEWKNAFDNDEFRKIFALDILENYIINK
                     RWYGGKASTLKYIEIVDHFKIASEKNTYYGVLIEVNFKEAFYQHYFMPLAFMAEEELD
                     TNTIIAPVQIGKQKGFLVDALHQEDFRKLLFENIVQAKENKALKLIFHKGLKFEDKEY
                     KSSKFMGLEQSNTSIIYNDAFVLKIFRRIYVSTNPDYEISRFLTERMNFESSPAYIGS
                     INLDLPEGNITLGLMQELVENQGDAWQFMLEELDGVFSNLKTKKIKIDKLPDIPLFKK
                     LKINEIPPEIIDWVGLSLFIKLQTLAKRTAEMHIALGSDIHDTAFTPTTYNGDYTVWL
                     KNRMLYQFQNRLNIIENSLHKLDGLALELAHQFMENKKLVRKHFVDFDWTKMKSERIR
                     IHGDYHLGQVLVNGDDFYILDFEGEPESTIRDRKVKQPPLKDVAGLFRSFHYAIYATI
                     FNNKDKYPFEQEQLFKAAELLFNYFVGAFLQTYVEKAQAGNLNIGYDHEIEFLLKYCL
                     IEKAVYELGYELNSRPRWAVIPLRGIQSIMKY"
     gene            5287..7191
                     /gene="glgB"
                     /locus_tag="PK218_08820"
     CDS             5287..7191
                     /gene="glgB"
                     /locus_tag="PK218_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPW98650.1"
                     /translation="MNQVQVHSLFTEFDINLFKAGKHFKLYEKLGAHVTEVNGVKGVY
                     FAVWAPAARSVSVVGDFNYWIQGEHILNVRWDGSGIWEGFIPGVDKGTTYKYKIQSNN
                     GGIVTEKADPFALYCEHPPQTASVIWDLEYKWKDKKWMDSRKDKNGLDKPFSVYEVHL
                     GSWRRNGDGKFLTYVEMAEQLVAYVKETGFTHVEFMPVMEYPYDPSWGYQLVGYFAPT
                     SRFGKPQDFMLLVDKLHQAGIGVILDWVPSHFPEDAHGLGFFDGSNLYEHPDRRKGFH
                     PDWKSLVFNYGRNEVRSFLISNAIFWLSQYHVDGLRVDAVASMLYLDYSRKDGEWETN
                     IYGGRENLDTISFLKDFNEAVYSNFPDVQTIAEESTSFPMVSRPTFLGGLGFGMKWMM
                     GWMHDTLQYFKKEPVYRRFHQNDLTFSMTYAFTENFMLPLSHDEVVYGKKSILGRMPG
                     DEWQRFANLRLLYGYMFTHPGTNLLFMGAEFGQSSEWNFEGSLDWHLLQFGYHEGVKK
                     CITDLNGLYKNNPAFYEKQFSAEGFEWINYQDAENAVLSYIRKGNDEKENMIVVCNFT
                     PVVRTDYKIGLATKGKLTELFNSDKVDYGGSGATNSKAIKIDKTPWNWKDYSAVITLP
                     PLGVVVFKIA"
     gene            7351..>9590
                     /locus_tag="PK218_08825"
     CDS             7351..>9590
                     /locus_tag="PK218_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPW98651.1"
                     /translation="MITNQELEFKGDQFPSKIVSFEQDVDSIFFYTDNNVILKTTILR
                     DSLIRFRYTTKGYFSNDFSYAIDKTQSHGYNQLEVIEDADSYSIKTSKVTCIITKNDL
                     KVSIYDLDGFAILEDELGFHWEECYEHGGNIVKMSKISKDGESFYGLGDKATHLNLKG
                     KRLENWATDQYAFQKDQEPLYKVVPFYIGLHHDKAYGVFFDNTFRTFFDFCHERRNIT
                     SFWAEGGEMNYYFFYGPKMSDVVKTYTHLTGKPELPPMWSLGYHQCKWSYYPESKVKE
                     VTSKFRELQIPCDAIYLDIDYMEGFRCFTWSKEYFPEPKRMVAELAEDGFKTVVIIDP
                     GIKIDKDYWVYQEALKNDYFCKRADGPFMKGKVWPGECNFPDYTNPEVREWWAGLFKE
                     LIAEIGVKGVWNDMNEPAVMDVPGKTFPNDVRHNYDGNNCSHRKAHNIYGTQMARATY
                     EGVKRFAYPKRPFIITRSAYSGAQRYTSSWTGDNVATWEHLWIANIQMQRMSISGMGF
                     TGSDIGGFAEQPSGELYARWIQLGVFHPFCRTHSSGDHGEQEPWSFGDEVIDITRKFV
                     NLRYQLLPYLYTMFWQYVNEGTPMLKPLVYFDQEDFHTHYRTDEFIFGNQILVCPILE
                     PNAQGRRMYLPKGNWYNYWTNELVEGKKELWVNTKFDEIPVFVKEGAIIPKYPVQQYV
                     GELQFEELTLDVYYKLGKEKSLVYEDANDGYDYNKGRFSLRTFTLTGKEKELIIQQHK
                     EGDFIT"
BASE COUNT         3168 a         1305 c         1905 g         3212 t
ORIGIN      
        1 tcgtcgcctt tttttgaaaa ccctattctt tatagaatag ggttttttgt ttttaacgac
       61 gtgctgatta taagttttta ccatttgatt taaaataatt acaacattta tttactacgc
      121 aaatgtcaca tttcgggttt gttggtcggc agatttctct acctagaaat gaaattgaca
      181 taccaatttc cccccaaata ctcttaggta atttttccat taattgtttt tcaactttgt
      241 ttccgtcttt gacttcggaa atgattccta ttcttggagc tacacgaata acatgtaaat
      301 ctgcaataat tccttctgcg ggtttattaa tttcccgtaa aattacattt gcagattttc
      361 tgccaattcc ttttagtgca attaagtcgg aaaggtttgt tgggatgttt ttgtcttctt
      421 ttaatttttg agcaatttgt attatccaac ttgcttttgt ctcaaagttt ctcacttttg
      481 aaatataggg tagtaaggct tcaggagttg aaatagctat gctttctata tttggaaacg
      541 ttttaaaaag aattggagaa attttgttaa tattagcatc agaatcttga gctgagagaa
      601 tgaccattat cattagttga taaagattat tgtattctaa tggatgtttt tttccatgat
      661 acaaatttat taacggatgt agattactag cccatctgtc ttgttcttca tttgaaacca
      721 taatttattc ttttaaaaat ttgatactat tttctataac tattttgtca cctagaattt
      781 ttcggtgccc aagtccttca gttatcatta attgactatt ctttagactt ttgtttattt
      841 gataagcagc tttcacatca acatcatcat catttttatc atgaattatt aaaattggtt
      901 gtgaaatatc tttagaaaca aagcttgatc cataatcttc cattggttcg ccatattttt
      961 tttcataata ttctttaagt ttgatgccta ttttatgatt taatccaatt ttttttacaa
     1021 aatcatcaat tatatcttga ataacatcgc cacttccaat tattatagct ttattaatag
     1081 taaagttttg ttttaaagcg tttaatattg ccataccgcc taaagaatgt cctatagcaa
     1141 attcaaatgg agcaaaatgt ttttctattt ctaaaataca tgccataaat tcaggcatta
     1201 gggttttatt tcctttagat tttccatgtg caggtgcgtc aaagctaata gtcgaatagc
     1261 cattttcaat taaagcatca gctattttat ataattgcgt tcctctgcct gaccaaccat
     1321 gtacaaggag tactttttta cttgatttgc catattcata tataactatt tcttttccaa
     1381 tttttggaat tttaatagtt gattgaatgc tatttctatt catattccat tctcgctttg
     1441 gaattttatg ttttattggt gtagcaaata attttgcggc aaataaagtt gttaattttg
     1501 ttgaaattgc ttctaaaaac ctactagtat atagaattcc tttcggaatt tgcaaagaat
     1561 gactggtttt atcttctttt tttatcattt caataaattt tactgcaatt tataacttat
     1621 tgtatttttt tctaacttac aactccttaa aattacaatt aaaaatgcaa aatcaaactc
     1681 gaattgttat tgaaaatgta aatccacaac tagatagtgg tgcttttttt attaaaagaa
     1741 ttataggtca aaaagtaatt gttactgcaa atgttttttc tgacggacat gatgttatag
     1801 ctagttctgt aaagtataaa catgaaaaag agaagaaatg gcaagaaatt agaatgcaag
     1861 aaaccggaaa tgatgaatgg atagctcaat ttaaagtaga aaaacaaggt ttctatactt
     1921 attttgttga aggttgggta gattatgctc taaattggca acatggcaca gagagaaaaa
     1981 ttaatgataa tcagcatgta aaatctgaat tattggaagg tgctgaatat tgtcaggcta
     2041 ttttaaaaga agtttctaaa gatgaaaaag cttttttgaa taatgcaata aaagcttttc
     2101 aagatgaaaa aaaatataat gaagccattg ctattgcttt atcaaaagag ttgcatgata
     2161 ttttttataa tcatcctacg agaactttag caaatagttc aattgaattg aaagtttatg
     2221 ttgatagaaa aaaagcgtta ttcagtacat ggtatgagtt ttttccacgt tcttcatcag
     2281 aagtaaaagg aaagcatgga acttttaaag actgtgaaaa attattacca agagttgcat
     2341 ccatgggatt tgatacattg tattttccac ctatacatcc aataggtgaa gtaaatcgaa
     2401 aagggaaaaa taatgctacg aatgctcaac ctggtgacgt aggttcgcct tggggaattg
     2461 gttcaaaaca cggtggacac aaatctactc atcctgaatt gggaacttta gaagatttta
     2521 agtcattggt aaaaaaagcc aaagaacttg gaattgaggt tgcaatggat tatgctttgc
     2581 aagccgcacc agatcatcct tatgtgaaag attttcctca gtggttcaaa tggcgaccag
     2641 atggaacagt tcaatatgct gaaaatcctc ccaaaaagta tcaagatatt caaccgatat
     2701 attttgaaag tggtgattgg aaaaaccttt ggaaagaatt actcgatgtt gctttgtatt
     2761 gggttgaaga atgtgatata aaagttttta gagtagataa tccgcataca aagccgtttt
     2821 atttttgggg ttggctgatt gctgaagtaa agaaaaagca tccagatgtg ttatttttgg
     2881 cagaagcatt tactcgtcct aagataatga atgaattggc aaaacaagga ttcacgcagt
     2941 catatactta ttacacatgg cgaaattcaa aagctgaatt gattgaatat ttagaagagt
     3001 taaccaaatc agagcaaaaa gaatatttcc gaccaaactt ctggcccgat attttgcctt
     3061 atgctttgca aagtgggatg gaaagtgttt atctgcacaa atactttttg gcagcaacat
     3121 tgagttcaag tgtgggactt tatggaccag tttatgaata tatgatttcg gatgcaatgc
     3181 cgggaaaaga agagtatttg aactctgaaa aatatgaagt gtatcattgg gattggacta
     3241 ttcaaaacaa attggtcact gtaatttctc gtttgaatag aattagacat gaacaaccat
     3301 cattgcaaca aacgaataat attgaatttt gtgcaaccga taatgatcaa gtgattgcct
     3361 attataaata tgacgatgat aaacttgacg aaacgttgat gatttgtaat attgatccct
     3421 attacccaaa acaagcttgg gtgcagttgc cattaaagtc tttaaaagtg caagcaggtc
     3481 aaccgattaa agtggtcgat ttggttactg gaaatagtta tatatgggat aaggaatgga
     3541 attttgtgga attacatcca tcattaccat tccatttgtt caagattatt aaataggtta
     3601 caataattta atgccaaact gatatgcaaa cctctagtga agaaactttt acttctccat
     3661 ttgaatttaa aaccgagtgg aaaaatgctt ttgataatga cgaatttaga aagattttcg
     3721 cattggatat tcttgaaaat tatattatca ataagcgatg gtatggcgga aaagcaagta
     3781 ctttaaaata tatagaaatt gttgatcatt ttaaaattgc ctctgaaaaa aacacttatt
     3841 atggtgtttt gattgaagtt aattttaagg aagcctttta tcagcattat tttatgccgt
     3901 tggcatttat ggcagaagaa gaattggata ctaatacgat aattgcgccg gttcagattg
     3961 gaaaacagaa aggttttttg gtagatgcgc tacaccaaga ggattttagg aaattattgt
     4021 ttgaaaatat cgttcaagca aaagaaaata aagctttgaa acttattttc cacaaaggat
     4081 tgaaatttga agacaaagaa tataaatctt cgaagtttat gggattggag cagagcaaca
     4141 cttctattat ttataatgat gcatttgtat taaagatttt tagaagaatt tatgtgagca
     4201 ccaatcctga ttatgagatt agccgatttt tgacggagcg gatgaatttt gaaagttcgc
     4261 ccgcttatat tggcagtatt aatttggatt tgcccgaggg aaatatcact ttaggtttga
     4321 tgcaagagtt ggttgaaaat cagggtgatg cttggcagtt tatgcttgag gaattggacg
     4381 gtgtttttag taatttgaaa acaaagaaaa ttaaaattga taaactaccc gatattcctt
     4441 tgtttaaaaa attaaaaata aacgaaattc ctcctgaaat tattgattgg gtaggattga
     4501 gtttgtttat taagttgcaa accttggcaa aacgtactgc tgaaatgcat attgctttgg
     4561 gaagcgacat acacgatacg gcttttacac ctacgactta taatggtgat tacacggttt
     4621 ggttgaaaaa ccgaatgttg tatcagtttc agaatagatt gaatattatt gagaacagtt
     4681 tgcataaatt ggatggattg gctttggaat tagcgcatca atttatggaa aacaagaaac
     4741 tggttcgtaa gcattttgtg gattttgatt ggaccaaaat gaagagtgaa cgcatcagaa
     4801 ttcatggcga ttatcatctt ggacaagtgt tggtgaatgg tgatgatttt tacatccttg
     4861 attttgaagg tgaacccgaa agcactattc gtgatagaaa ggtgaaacag ccgccgttga
     4921 aagatgttgc aggtttgttc cgttcgtttc attatgctat ttatgcgacg atttttaata
     4981 ataaagacaa atatcctttt gagcaagagc aattgtttaa ggcagccgaa ctattgttta
     5041 attattttgt tggtgctttt ttgcaaacgt atgtagagaa ggcgcaagca ggcaatttga
     5101 acattggcta tgaccatgag attgagtttt tattgaaata ttgtttgata gagaaagcgg
     5161 tatatgagtt gggctatgag ttgaactcga gaccacgctg ggcggttatt cctttgcgag
     5221 gaattcagag tataatgaaa tattaaaaat ataaagaaac atttatagtt tcaaaaaaca
     5281 ataaaaatga accaagtaca agtacattcg ctttttacag aatttgatat taatttattc
     5341 aaggcaggaa aacattttaa gctttatgaa aaactaggtg cgcatgttac cgaagtaaat
     5401 ggagtgaaag gagtttattt tgcggtttgg gcaccagcgg cacggtcggt ttcggtagtt
     5461 ggtgatttta attattggat acaaggcgaa catattttga atgtgcgctg ggatggttct
     5521 ggaatttggg aaggatttat tccgggtgtt gataaaggaa cgacgtataa gtataaaatt
     5581 cagtcgaata atggcgggat tgttactgag aaagccgatc cgtttgcttt gtattgtgag
     5641 catccaccac aaactgcttc ggttatttgg gatttggagt ataaatggaa ggacaaaaaa
     5701 tggatggaca gccgaaaaga taagaatggt ttggacaaac ctttttcggt atatgaagtg
     5761 catttgggtt cgtggcgcag gaatggtgat ggaaagtttt tgacgtatgt tgaaatggcg
     5821 gagcaattgg ttgcctatgt aaaggaaact ggttttacgc atgtggagtt tatgcctgtg
     5881 atggaatatc cttatgaccc atcgtggggt tatcagttgg tgggttattt tgcgccaact
     5941 tcgcgatttg gaaagccgca ggattttatg cttttggtgg ataaattaca tcaggcggga
     6001 attggagtga ttttggattg ggtgccgtcg cattttcctg aagatgcgca tggtttgggc
     6061 ttttttgatg gttcaaactt atatgaacat cccgatagac gaaaaggatt tcatccggat
     6121 tggaagagtt tagtctttaa ttatggacga aatgaagtgc gttcgttctt gataagtaat
     6181 gcgatatttt ggttaagtca atatcatgtt gatggactgc gcgttgatgc agtggcttcg
     6241 atgttgtatt tagactattc aagaaaagat ggcgaatggg aaacgaatat ttatggagga
     6301 agagaaaatt tggatacgat tagtttctta aaagatttta atgaagccgt gtattctaat
     6361 tttccagatg tgcaaactat agccgaagaa agtacaagct ttccaatggt ttctagacca
     6421 acatttcttg gaggattagg atttgggatg aaatggatga tgggttggat gcacgatacg
     6481 ttgcaatatt ttaaaaaaga accagtttac cgtcgttttc atcaaaatga tttaacgttt
     6541 tcgatgacgt atgcgtttac agagaatttt atgttgccgt tatcgcatga tgaagtagtt
     6601 tatggaaaaa aatctatttt aggaagaatg cctggcgatg aatggcagcg ttttgcaaat
     6661 cttcgtttgt tatacggtta tatgtttacg catcctggaa cgaatttgtt atttatgggc
     6721 gcagaatttg ggcaaagtag tgaatggaat tttgaaggaa gtttagattg gcatttattg
     6781 caatttggtt atcatgaagg cgtgaaaaaa tgtatcacag atttgaatgg gttgtacaaa
     6841 aacaatcctg cgttttatga aaaacaattt tcggcagaag gatttgagtg gatcaattat
     6901 caagatgctg aaaatgccgt gttaagctat atccgaaaag gaaacgatga aaaggaaaat
     6961 atgattgttg tttgtaattt tacgccagta gttcgaacag attataagat aggattggca
     7021 accaaaggaa agttaacaga actatttaac tcggataaag tagattatgg cggaagtggc
     7081 gctactaact ctaaagccat aaaaattgat aaaacacctt ggaactggaa agattattca
     7141 gccgtaatta cgttgccacc attgggtgta gtggttttta aaatagctta aattataaaa
     7201 gtaaattcaa atgaagttgg ttattttagt aaccaacttc tttttttagt tatcattata
     7261 cgaaaacgtt tgagttgaaa actacttaga tttctttttg tcatttcgtt ttcaactttg
     7321 taaatttggc aattacctaa aataatatac atgattacta accaagaact tgaatttaaa
     7381 ggcgatcaat ttccatcgaa aatagtttca tttgaacaag atgtagatag tatttttttc
     7441 tatactgaca ataatgtaat tcttaaaaca acaatacttc gcgatagttt aattcgattt
     7501 cgatatacaa ccaaaggata ttttagcaat gatttttctt atgccattga taaaacacaa
     7561 tcacacggtt acaatcaatt agaggttatt gaagatgctg atagttattc gattaaaaca
     7621 agcaaagtta cttgtatcat tactaaaaat gacttaaaag tatccattta tgatttggat
     7681 ggttttgcta ttttggaaga tgaacttggt tttcattggg aagaatgtta cgaacacggt
     7741 ggaaacatcg taaaaatgag taaaatttca aaagatggtg aaagttttta tggtttgggc
     7801 gataaagcaa cgcatctaaa cttaaaagga aaacgattgg aaaattgggc aaccgatcaa
     7861 tatgcattcc aaaaagatca agaaccattg tataaagttg ttccatttta tattggtttg
     7921 catcacgata aagcctacgg agttttcttt gataatacat ttagaacatt tttcgacttt
     7981 tgtcatgaac gtagaaacat aactagcttt tgggcagaag gaggtgaaat gaattattac
     8041 tttttctatg gaccaaagat gagtgatgtt gtaaaaacat acacacattt aacaggaaaa
     8101 ccagaattac ccccaatgtg gtcgctagga taccatcaat gtaaatggag ttattatcca
     8161 gaaagtaaag taaaagaagt taccagtaaa tttagagaat tgcaaattcc ttgtgatgcc
     8221 atctatttag atattgatta tatggaaggt tttcgctgtt ttacttggag caaagagtat
     8281 tttccagaac ccaaaagaat ggttgccgaa ttggcagaag atggttttaa aaccgtcgtc
     8341 atcattgatc caggaataaa aatcgacaaa gattattggg tttatcaaga agctttgaag
     8401 aatgattatt tttgtaaaag agccgatgga ccatttatga aaggaaaagt ttggcctggg
     8461 gaatgtaatt tcccagatta taccaatcca gaagttcgtg aatggtgggc aggattattc
     8521 aaggaattga ttgctgaaat aggagtaaaa ggtgtttgga acgatatgaa cgaacctgcg
     8581 gttatggatg ttcctggaaa aacttttcca aatgatgttc gacacaatta tgatggaaat
     8641 aattgcagtc acagaaaagc acacaacatt tatggaacgc aaatggcaag agcaacttat
     8701 gaaggtgtaa aacgatttgc atacccaaaa agaccattta ttataactcg ttcagcttat
     8761 tctggtgcgc aacgctatac ttcatcatgg acaggagata atgttgctac ttgggaacat
     8821 ttatggattg ctaatattca aatgcaaagg atgtcgattt ctggaatggg ttttacaggt
     8881 tctgatattg gcggatttgc cgaacaacca tctggtgaat tatacgctcg ttggattcag
     8941 ttaggcgttt ttcatccgtt ttgtagaacc cattcatcgg gtgatcatgg tgaacaagaa
     9001 ccttggtcgt ttggtgatga agtgattgat attactagaa aatttgtcaa cttacggtat
     9061 caacttttac cttatttata tacgatgttt tggcaatatg tcaatgaagg tacgccaatg
     9121 ttgaaaccat tagtttattt tgaccaagaa gattttcata cccattacag aacagatgaa
     9181 tttatttttg gaaatcaaat attggtttgc ccaatattag aaccgaatgc gcaaggaaga
     9241 agaatgtatt tgccaaaagg aaattggtac aactattgga caaatgaatt ggtagaagga
     9301 aaaaaagaac tttgggttaa tacaaagttt gatgaaattc cagtatttgt aaaagaaggt
     9361 gctattattc ctaaatatcc tgttcaacaa tacgttggcg aattacaatt tgaagaatta
     9421 actttggatg tatattataa actgggaaaa gaaaaatcat tagtttacga agatgctaac
     9481 gatggatatg attataacaa aggaagattt tcgttgagaa cctttacttt gacaggaaaa
     9541 gagaaagagt taattattca gcaacacaaa gaaggcgatt ttattacaca
//
LOCUS       DAOOBH010000088         9520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_8961_length_9520_cov_6.043212, whole genome shotgun sequence.
ACCESSION   DAOOBH010000088 DAOOBH010000000
VERSION     DAOOBH010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8961_length_9520_cov_6.043212"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /locus_tag="PK218_08830"
     CDS             <1..1674
                     /locus_tag="PK218_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98652.1"
                     /translation="ICPTVPGQLVTVTFTAFDTEATWDALYVFDGNSINAPQIASTNG
                     AGNVPGGLSGGYWGAVIPGPFTSTSPDGCLTFRFRSDGSVSNPGWVANVTCGNVDRVI
                     LNAFIDTNANGIKDTDEIYFNHGSFEYDTNNSGTNTEVYSPTGYYVITDDNGTNSYDI
                     GYQIQPEYTAYFSAGSTAYTDITIPIGSGTQNLNFPITITQTYTDVAVTIVPINAPRA
                     GITNYRNEVIVSNYGNETIATTTLTFTKDSNLTIVNTSDAGAISIPTGFTRTFTDLLP
                     NESRSIIINMSVPSIPTVSIGQVLTNTATVTPPTNDLLVNNNNATLSQPIVAAYDPND
                     KLESHGGRIQFDSFTQNDFLVYTIRFENTGNTSALNVKVTDILDSRIDETSLRMLSAS
                     HSYLLTRNGANLLWDFKDILLPVSIPNTQLGKGFITFKVKLKPGFAIGDIIPNTANIF
                     FDTNPAIVTNTFNTEFVQSLSNSSFADSDLVVYPNPASSYVQIALNNATQNIRSIVIY
                     DLLGKKVLETNSVTSNEITLNTSSLIKGVYLLEINANSNERLVKKLVIQ"
     gene            complement(1729..2202)
                     /locus_tag="PK218_08835"
     CDS             complement(1729..2202)
                     /locus_tag="PK218_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPW98653.1"
                     /translation="MQNILRIATVIMTCLAVGYLSSIVTQENIPTWYANINKPSFNPP
                     NWIFAPVWTMLYVLMGYAGGRIWNKIGINEAAVKKAFLFFLIQLALNALWSFLFFGLH
                     NFMLASIEIIVLWLMIYETMLQFKKIDKVAAYLLIPYIIWVSFATLLTFSIWNLN"
     gene            2277..3383
                     /locus_tag="PK218_08840"
     CDS             2277..3383
                     /locus_tag="PK218_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HPW98654.1"
                     /translation="MFSEKDFIFNTTTFEINKGSFQWSAPSNIALVKYWGKKEHQIPA
                     NPSISFTLNQCKTITKLSFERKNTSTALSETKKNFSFDLLFEGKPKVDFKPKIQKFFE
                     RIEVYCPYLKHYHFIIDTENTFPHSSGIASSASGMAALAMNIMSLEKALNPEMTKEYF
                     TQKASFLARLGSGSACRSIKGNVVVWGKHTGINGSSDLFGVEFAEKIHQNFKNYQDTI
                     LLVDKGEKQVSSTVGHDLMHNHPFAKQRFEQAHTNLSSIKKVLENGNIEEFIKIVESE
                     ALTLHAMMMTSMPYFILMKPNTLEIINRIWKFRAETKTPVCFTLDAGANVHVLYPEND
                     REHILQFIKDELIGFCQNANYICDTIGNGAIQIL"
     gene            3380..4318
                     /locus_tag="PK218_08845"
     CDS             3380..4318
                     /locus_tag="PK218_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HPW98655.1"
                     /translation="MKGPLFYSKILLFGEYGIIKDSKGLSIPYNFYNGALKVDENPSA
                     EAIKSNESLQRFVTYLEQLQNDQPELATFNLKDLKSDVESGMYFDSSIPQGYGVGSSG
                     ALVAAIYDKYAANKITVLENLTREKLLQLKSIFSSMESFFHGKSSGLDPLNSYLSIPI
                     LINSKDNIEATGIPSQTADGKGAVFLLDSGIVGETAPMVSIFMENLKDQGFRRMLKNQ
                     FVKHTDACVENFLGGDMKSLFSNTKKLSKVVLNHFKPMIPEQFHGVWQKGIDTNDYYL
                     KLCGSGGGGYILGFTENLEKAKQSLKDYKLEVVYQF"
     gene            4334..5251
                     /locus_tag="PK218_08850"
     CDS             4334..5251
                     /locus_tag="PK218_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HPW98656.1"
                     /translation="MLSRKNKLLVMKIISLFSVVRGYNIPIIVLAQYLSAIFILAPNK
                     RALEVLLDVNLFLIVFLSSLTIASGYIINNFYDNDKDLINRPNKSMLDRLVSQKTKLY
                     VYFSINFIVFLLAFFISFRAVLFFSIYIFLIWFYSHKLKKMVVIGNLFAAFLAVLPFF
                     AILLYYKNLYPQIFAHATFLYLLLLIREMIKDLENLKGDIANDYKTIPVLFGEKISKQ
                     IISFLTFGTIIPVYYLVTIFEVGYMDIYFYLSLIVLLFFLQLLWKSNDKSGYLKLHNI
                     LKFLIVSGVFCIVLIEPEVLIHGKRLLKL"
     gene            5304..6215
                     /locus_tag="PK218_08855"
     CDS             5304..6215
                     /locus_tag="PK218_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW98657.1"
                     /translation="MNKKEGNNKRNSSRPNSSRPSSNKPKPAMAKRAQGPKKAKPTTD
                     DSLPKRTPKSTKPVANNTPKPLKSDDIRLNKYISNSGMCSRRDADIYIQSGNVKVNGQ
                     VIVEMGYKVKPGDVVNFDGAVITPERKEYIVLNKPKNFTTSNEDEADHRNVMELVRGA
                     TKASIQPIGRMDKNTTGLLVFTNDTDMIRKFSLPNQKSTKIYQVSLDKNLKFEDLEKI
                     NGGVTLDGHRLYVDEISYIENEPKTEIGIKLRTSNVKVVRSIFESFNYNVLKVDRVSF
                     SGLTKKNLPRGNWRFLTEQEIINLKNV"
     gene            6274..6636
                     /locus_tag="PK218_08860"
     CDS             6274..6636
                     /locus_tag="PK218_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW98658.1"
                     /translation="MATEKNLSIAHLWFEAFNAHNLEKLLSLYDDEAQHFSPKLKIRH
                     PETYGLVVGKESLRTWWQDAFERLPSLHYKVTSLTSNSDRVFMEYIRTVTNEENMLVA
                     EVLEIKEGKIIASRVYHG"
     gene            6705..8474
                     /locus_tag="PK218_08865"
     CDS             6705..8474
                     /locus_tag="PK218_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HPW98659.1"
                     /translation="MTKTHSFFTLFLISIVMIGCKSEEKKPVDFKSITDAYFKEKNGL
                     NPLEATQNGQSDFNDQLVFEMTDSYRKHKKEVFEKVVKDLNSVDYETLSNEEKNSFDI
                     MKWEADIELDLLKQNANLTPIQQFWGTHITMAQYAAGESAQPFKTQKDYTNFLKRIDL
                     YSVWIDSAIVYMKKGIDEKVVLPKVLAEKVVPQFEALITPNVEDNLFFSSVKKFPADF
                     TETQKSDFSKKYATSITEKLVPQFEKMIRFLKNEYIPATRNTSGIGSIKGGNDLYKIY
                     VKQWTTTTETPEDIHKLGLSEVARIKTEMEKVKNQVGFKGSLIEFFEYVRNKSELMPF
                     SKPEEVIANFERIHTAIKPNVDKLFSLQPKTPFQIKRTEAFREKTASAEYNQGAADGS
                     RPGTFYVPIPDVKKYNCYGDEDLFLHEAIPGHHFQISLQQENQNLPDFRKFSWFGAYG
                     EGWALYTESLGKELGLYTDPYQYFGMLGNEMHRAVRLVVDTGIHSKGWTREHAINYSL
                     ENEAESESSIISEIERYMAIPGQALSYKIGQLKILELRKKAQNKLKDKFDIKVFHQKV
                     LESGVMPLALLEKKINHWIASGV"
     gene            complement(8501..9262)
                     /locus_tag="PK218_08870"
     CDS             complement(8501..9262)
                     /locus_tag="PK218_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98660.1"
                     /translation="MNYSLMEILLIALLGCFFIRLLFYFLSERSRKASEKVAEKTIAL
                     AKATKEIEMLNIQLEDKSSFKDSSDSNYKKVNSYESMLGNNLPIIEITDLDLSEETTV
                     DINDENSNRPAFVHQLRFYVFNVGKSSLKEVYISIKDIYNDPKDIKKRSRTIGQHDDE
                     SIDFKDYGIGTFENFEIGTLNLKSRRLVYASTLPNSFGVAEYSFSVVVEWKGGFYQMD
                     VRIEEFDGKLKYFYQYFDVSENPINFELLESNIKK"
BASE COUNT         3401 a         1527 c         1516 g         3076 t
ORIGIN      
        1 atttgcccaa ccgtacctgg acaattagta acagtaactt ttactgcctt tgacaccgaa
       61 gcaacttggg atgcgttata tgtatttgac ggaaactcta taaatgcacc acaaatagca
      121 agtacaaatg gtgctggcaa tgttcctgga ggattatcag gtggttattg gggagcagtt
      181 atccctggtc cgtttacttc tacaagtcca gatggttgtt tgacctttag atttagaagt
      241 gatggctcag tttcaaatcc aggttgggtt gccaatgtta catgtggtaa tgtagataga
      301 gttatattaa atgcttttat agatacaaat gctaatggta ttaaagacac tgatgagata
      361 tactttaatc atggttcgtt tgaatatgac actaataatt cgggcaccaa tacagaggta
      421 tattctccaa caggctatta tgtaatcact gacgataacg gtacgaattc gtatgatatt
      481 ggatatcaaa tccaaccaga atatacggca tactttagtg ctggttcgac cgcttatact
      541 gacattacta ttcctatagg cagtggaaca caaaatttaa acttcccaat aactattaca
      601 caaacttata cggatgtggc ggtaactatc gtacctataa atgctcctag agcaggaatt
      661 actaattata gaaatgaagt tatagtatca aattacggca atgaaactat tgctacgact
      721 accttgacat ttacaaaaga cagtaatctg accattgtaa acacatcaga tgctggtgcc
      781 atatcaatac ctacaggttt cactaggacg tttactgatt tgcttccaaa cgaaagtaga
      841 agcattatta ttaacatgag tgttccatca attccaacgg taagtattgg tcaggtattg
      901 acaaatactg caacagtaac gccacctacg aatgacttat tagtaaataa taataacgcc
      961 acacttagcc aacctattgt tgctgcatat gatccaaacg ataaattgga atctcatggt
     1021 ggcagaatac aatttgattc ctttactcaa aacgattttt tagtttacac tattcgattt
     1081 gaaaacactg ggaatacaag tgctctaaat gtaaaagtta cggatatttt ggatagtaga
     1141 attgatgaga cttcattgag aatgttatct gccagtcaca gttatctttt aacacgaaat
     1201 ggtgctaatt tgctatggga ttttaaagat attctcttgc ccgtttctat tcctaataca
     1261 caattgggga aaggctttat tactttcaaa gtgaaactta aaccaggttt tgctattgga
     1321 gatattattc caaatacggc taatatattc tttgatacca atccggcgat tgtaacgaat
     1381 acttttaaca cagagtttgt tcaaagttta agtaattcat catttgccga tagtgatttg
     1441 gtagtttatc caaatccagc atcaagttat gttcaaattg cattaaataa tgcaacccaa
     1501 aatatacgtt caattgtaat atacgatttg cttggcaaaa aagttttgga aaccaatagc
     1561 gtaacatcca atgaaataac attgaacaca tcatcattaa taaaaggagt ttatttgtta
     1621 gaaataaatg ctaactcgaa tgaaagattg gtgaaaaaat tagttattca ataacaccct
     1681 ataaccttat ttcataaaaa agttcgataa ttgtatcgaa cttttttatt aatttaagtt
     1741 ccatatagaa aaagttaata aagtagcaaa actgacccag atgatataag gaataagcaa
     1801 ataagctgca actttatcaa tctttttaaa ttgcaacatc gtttcataaa tcatcaacca
     1861 aagtacaatg atttcaattg aagccaacat aaaattgtgc aatccaaaga acaaaaatga
     1921 ccacaaagca ttcaaagcta attgaattaa aaaaaacaaa aaagcttttt taacggcagc
     1981 ttcatttatt cctattttat tccaaatacg tccgccagca tagcccatta atacataaag
     2041 cattgtccat acaggtgcaa aaatccaatt gggtggatta aaacttggtt tgttaatatt
     2101 ggcataccaa gtaggaatat tttcttgagt aacaatactt gataaataac ctacagcaag
     2161 acaagtcata atcactgtag ctattcgtaa aatgttttgc ataaatagtt tttatccaaa
     2221 tatactacac aatttattat tctactaaaa aaagtatctt tgtcaaaaat tcgagcatgt
     2281 tttcagaaaa ggattttatc ttcaatacaa ccacttttga aataaacaaa ggaagttttc
     2341 aatggtcagc tccaagtaat atagcattag tcaaatattg gggtaaaaaa gaacatcaaa
     2401 ttcctgccaa tccttccatt agtttcactt taaatcaatg caaaaccatt actaaattgt
     2461 cttttgaaag gaaaaacact tcgactgcgc tcagtgaaac caaaaaaaac ttttcatttg
     2521 atttattatt tgaaggaaaa cctaaagtag attttaaacc caaaattcaa aaattctttg
     2581 aacgaataga agtgtattgt ccctacttaa aacactatca ttttattatt gatacagaaa
     2641 acacttttcc tcatagttct ggaatagcgt cttcagcctc aggaatggct gctttagcaa
     2701 tgaacatcat gagtttggaa aaagcattaa atcctgaaat gaccaaagaa tactttacac
     2761 aaaaagcctc ttttttagct cgtttgggtt caggaagtgc atgcagaagt atcaaaggaa
     2821 atgttgttgt ttggggaaaa catactggaa ttaatggtag ttcggattta tttggtgttg
     2881 aatttgctga aaaaatacat caaaatttca agaattatca agatactatt ttattggttg
     2941 ataaaggcga aaaacaagtt tctagtaccg ttggtcatga tttgatgcac aatcatcctt
     3001 ttgcaaaaca acgatttgaa caagcacata ctaatttgtc ttctatcaaa aaagttttag
     3061 aaaatggaaa tatagaagaa tttatcaaaa ttgtagaaag tgaagctttg acacttcatg
     3121 ccatgatgat gacttctatg ccttatttta ttttgatgaa acctaataca ttagaaatca
     3181 taaatcgtat ttggaagttt agagccgaaa ctaaaacacc agtttgtttc acgcttgatg
     3241 caggagcaaa cgtacatgtt ttgtatcccg aaaacgatag agaacacatt ttacaattta
     3301 ttaaggacga attaattggc ttttgtcaaa atgctaacta tatttgtgat acaattggta
     3361 acggagcaat tcaaatttta tgaaaggacc attattttac tcaaaaattc ttctcttcgg
     3421 agaatatgga attattaaag attctaaggg tttgtctatt ccttataatt tctacaatgg
     3481 tgcgttaaaa gtagatgaaa atccttctgc cgaagccatc aaatctaatg aaagtttaca
     3541 acgatttgtt acttatttag agcaattaca aaacgatcaa cctgagttag caaccttcaa
     3601 tttaaaggat ttaaaatcag atgttgaaag cggaatgtat tttgactcaa gcattcctca
     3661 aggctatggt gttggaagta gtggtgcact tgttgctgct atttatgaca aatatgctgc
     3721 taataaaatc accgttttag aaaacttaac ccgtgaaaaa ctgttacaat taaaatcaat
     3781 tttttccagt atggaatcat ttttccacgg aaaaagttct ggtcttgatc ctttaaacag
     3841 ctatttgagc attcctattt taatcaactc caaagataat attgaagcaa caggaattcc
     3901 gtctcaaact gctgatggaa aaggtgctgt gtttttatta gattcaggta ttgttggcga
     3961 aactgctcca atggtaagta tttttatgga aaacttaaaa gaccaaggtt tccgcagaat
     4021 gctgaaaaac caatttgtaa aacacactga tgcttgtgta gaaaatttcc ttggtggcga
     4081 tatgaaatcg ttgttttcaa atactaagaa gctgtctaaa gtcgttttga accatttcaa
     4141 acctatgatt cctgaacagt ttcatggcgt ttggcaaaaa ggaattgaca caaacgatta
     4201 ctacttaaaa ctttgtggtt ccggtggagg tggttatatt ttaggattta cagaaaattt
     4261 agaaaaagca aaacaatcac ttaaagacta taagctagaa gtagtttatc agttttaatc
     4321 tcaaaaaaag acaatgctta gccgtaaaaa caaacttttg gtaatgaaaa ttattagttt
     4381 gttctcggta gttcgaggct ataatattcc cattattgtt ctagcgcaat atctttctgc
     4441 catttttatt ttagctccaa acaaaagagc tttagaagta ttactagatg taaatttatt
     4501 tctgattgtt tttctttcgt ctttaaccat tgcatctggc tacattatca ataattttta
     4561 cgacaacgat aaagatctaa ttaacaggcc aaacaaatca atgttagata gattagttag
     4621 tcaaaaaacg aagctttatg tttatttttc tataaatttc atcgtttttc tacttgcatt
     4681 tttcatctct tttcgagcag ttttattctt ttcaatctat atatttctaa tttggtttta
     4741 ttctcataag cttaaaaaaa tggttgtcat aggcaattta tttgctgctt ttttagccgt
     4801 attacccttt tttgctatac tgttgtatta taaaaatctc tatccacaaa tttttgccca
     4861 cgcaactttt ctatatcttt tgcttttaat ccgagaaatg attaaagatt tagaaaattt
     4921 aaaaggcgat attgccaacg attataaaac cattccagta ctttttggtg aaaaaatttc
     4981 caaacaaatt atctcttttt taacctttgg aaccatcata cctgtttatt atttagtcac
     5041 tatttttgaa gttggctata tggatatata tttttatttg agtttaatcg tattgctttt
     5101 ctttttacag ttactctgga aatccaacga caaatcaggc tatttaaaac ttcataacat
     5161 cttaaaattc ctaatagttt ctggtgtatt ttgcattgta cttatcgaac ccgaagttct
     5221 cattcatgga aaacgattat tgaaattata attttttaat tatcaactct agaaaagcta
     5281 tctttgcaca aattctagaa tttatgaaca agaaggaagg caataataaa cgaaacagtt
     5341 cacgaccaaa tagttcgaga ccaagttcga ataagccaaa acctgctatg gccaaacgtg
     5401 ctcaaggtcc aaaaaaagcg aaacccacaa cagatgattc gttgccaaag agaacgccaa
     5461 aatcaactaa accagttgca aacaatactc caaaaccatt aaaatcagat gatattcgat
     5521 tgaataaata tatttctaat tcaggaatgt gttcacgtcg tgatgcagat atttacattc
     5581 aatcgggaaa tgtaaaagtg aatggtcaag ttattgttga aatgggatac aaagtaaaac
     5641 ctggtgatgt ggttaatttt gatggagcag taattacgcc tgaacgaaaa gaatatattg
     5701 ttttaaacaa acccaaaaac ttcacaactt ctaacgaaga tgaagcagac cacagaaacg
     5761 tgatggaatt ggttcgtgga gcaactaaag ctagcatcca acctattgga agaatggata
     5821 aaaacactac gggcttattg gttttcacca acgatactga catgattaga aaattcagtt
     5881 tacctaatca aaaatcaacc aaaatttatc aagtttcttt agataaaaat ttaaaatttg
     5941 aagatttaga aaaaattaat ggtggtgtaa cccttgatgg tcatcgtctt tatgttgatg
     6001 aaattagtta tattgaaaat gaaccaaaaa ctgaaatcgg tatcaaattg agaacttcta
     6061 acgtaaaagt agttcgttca atctttgaaa gttttaacta caatgtgtta aaagttgacc
     6121 gtgtctcttt ttctggatta accaaaaaga atttaccgcg tggcaattgg cgttttctta
     6181 ccgaacaaga aataattaat ttgaaaaacg tataaaaaat tatctttact accatgaaat
     6241 cccatttgaa tgggattttt ttatttaaaa cttatggcta cagaaaaaaa tttatcgatt
     6301 gcacatcttt ggttcgaggc atttaacgca cataatcttg aaaaattatt gtcgctttat
     6361 gatgacgaag cacaacattt tagtccaaaa ttaaaaattc gccatccaga aacctatggg
     6421 ttggttgttg gcaaagagtc attacgaact tggtggcaag atgcatttga acgtttgcca
     6481 agtttgcatt ataaagtaac ttcacttact tcaaattctg atagagtttt tatggaatac
     6541 attcgaaccg taaccaatga agaaaatatg ttggtagccg aagtactcga aattaaagaa
     6601 gggaaaatca tagcttcaag agtttatcat ggataattta tatttttgtc aacggttcat
     6661 tttcaacata actcttattt ttacgcaaac ctattccatt aatcatgaca aaaacacact
     6721 cttttttcac cctcttttta atttcaatag tgatgattgg ttgtaaatct gaagaaaaaa
     6781 aaccagttga ttttaaatca attactgatg cttattttaa agaaaaaaac ggtttaaatc
     6841 cactcgaagc aacacaaaat ggccaatcag attttaatga ccaattggtt tttgaaatga
     6901 ccgattcgta tagaaaacac aaaaaagaag tttttgaaaa agtagtaaaa gatttgaata
     6961 gtgttgatta tgaaacactt tctaatgagg aaaaaaacag tttcgacatt atgaaatggg
     7021 aagctgatat tgaacttgat ttgttaaaac aaaatgccaa tttaactcca attcaacaat
     7081 tttggggaac acatataacc atggcacaat atgctgctgg agaaagtgct caacctttta
     7141 aaacccaaaa agattatact aattttttaa aaagaataga tttatattca gtttggattg
     7201 actcagcaat tgtttacatg aaaaaaggaa ttgatgagaa agtcgtttta ccaaaagttt
     7261 tggcagaaaa agtggtgcct caatttgaag cattgattac accaaatgta gaagataatt
     7321 tatttttttc aagtgtaaag aaatttccag cggattttac tgaaactcaa aaaagtgatt
     7381 tttcaaaaaa atatgctaca agtattaccg aaaaattagt gccacaattt gaaaagatga
     7441 tacgcttttt aaaaaatgaa tacattccgg caacgagaaa caccagtgga attggaagca
     7501 taaaaggcgg aaatgattta tacaaaatat atgtaaaaca atggacaact actacagaaa
     7561 ctccagaaga cattcataaa cttggtctta gcgaagttgc tcggattaaa actgaaatgg
     7621 aaaaagtaaa aaatcaagta gggttcaaag gtagcttaat cgaatttttt gaatatgtaa
     7681 gaaacaaatc tgaattaatg cccttttcta aaccagaaga agtaattgct aactttgaaa
     7741 gaatacatac cgctattaaa cccaatgttg ataaattatt ttctttgcaa cccaaaactc
     7801 cttttcaaat taaacgcacc gaagccttta gagaaaaaac agccagcgca gaatacaatc
     7861 aaggcgcagc agatggttca agaccaggga ctttttatgt gccaattcca gatgtaaaaa
     7921 aatacaattg ttatggtgac gaagatttat ttcttcatga agctattcct gggcatcatt
     7981 ttcaaatttc gttacaacaa gaaaatcaaa acctgcccga ctttagaaag ttcagttggt
     8041 ttggtgctta tggcgaaggc tgggctttat atactgagag tttaggaaaa gaattagggt
     8101 tatatactga tccttaccaa tattttggaa tgttaggaaa cgaaatgcat agagccgtca
     8161 gattagttgt tgataccgga attcacagta aaggttggac aagagaacat gccataaact
     8221 attctttaga aaatgaagcc gaaagtgaaa gcagtatcat ttcagaaatt gaacgttata
     8281 tggcaattcc aggacaagca ctatcataca aaattggtca attaaaaata ttagaattgc
     8341 gaaaaaaagc gcaaaataaa ctaaaagaca aatttgacat caaagtgttt catcaaaaag
     8401 ttttggaatc aggagtaatg ccattggcac ttttagagaa gaaaattaat cattggattg
     8461 caagtggtgt ataaagtaaa agttcctcgt ttgaggaatt ttacttttta atgttacttt
     8521 ctaataattc aaaatttatt ggattttcac tgacatcaaa atactgatag aaatacttta
     8581 atttcccatc aaattcttca attctaacat ccatttgata gaatcctcct ttccattcaa
     8641 caaccacact aaaagaatat tcggccactc caaaactgtt tggcaaagtg ctagcataaa
     8701 ccaatcgtct tgattttaaa tttaaagttc ctatttcaaa attctcaaaa gttccaattc
     8761 cataatcttt aaagtctata ctttcatcat catgttgccc aatagttcga cttcttttct
     8821 ttatgtcttt tgggtcattg taaatgtctt taattgaaat ataaacttct tttaaagaac
     8881 ttttgccaac gttaaaaaca taaaaccgta gttgatgcac aaatgctggt cgatttgaat
     8941 tttcatcatt tatatcaaca gtagtttctt cggataaatc taagtctgta atttctatta
     9001 ttggtaaatt atttccaagc atactttcat agctattaac ttttttgtag tttgaatccg
     9061 aactgtcttt aaacgacgat ttatcttcta gttggatgtt caacatctct atttctttag
     9121 tagcctttgc aagagcaatc gttttttcag ctactttttc acttgctttt ctgcttcttt
     9181 cagacaaaaa ataaaacagc aatcttatga aaaagcatcc caaaagtgct atcaacagta
     9241 tttccattaa tgaataattc ataaaactga ttttgagtta ctagattgtt tccagaagta
     9301 aaattatctc aaaagggttt aggaataaat ttttgacgat aaatagctca atttttagtt
     9361 aaaattcaaa gtagaaactt taagtgcaaa aaaaatagat gaatggtata aaaaaaagct
     9421 caaactttcg tttgagcttg tagtgcgggt aataggactc gaacctacac gccttgcggc
     9481 accagatcct aagtctggca tgtctaccaa tttcaccata
//
LOCUS       DAOOBH010000089         9296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_9195_length_9296_cov_5.595931, whole genome shotgun sequence.
ACCESSION   DAOOBH010000089 DAOOBH010000000
VERSION     DAOOBH010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9296
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9195_length_9296_cov_5.595931"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1848
                     /locus_tag="PK218_08875"
     CDS             <1..1848
                     /locus_tag="PK218_08875"
                     /inference="COORDINATES: protein motif:HMM:NF033708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS sorting signal type C domain-containing
                     protein"
                     /protein_id="HPW98661.1"
                     /translation="TGTYYIQWIMTARSGGSFYFDDVSIVSGTPEVKTPIAGSPFTIN
                     AASTLYNVTGLNPVTAYHYVVRAVLGAVISPNSNEINVTTTLGTCTWDGSAWSNTTGP
                     TVSIAAIIEGAYDTSTYGGFTAKAVTINTGGSLTVNSGDTVTIVNSLTNSLTAAAVVV
                     ENNANLLQNGSSNQNSGSITVKRYSSALKRQDYTLWSSPVAGQQLQSFSPGTLSNRFY
                     TYDTSADQYAAVATPSATNFATATGYLIRMPNVYPTTATVWTDGAFVGVPNSGDYNFT
                     MVDGGSGLRFNLVGNPYPSPIDAADFVGNTTNATNTTGTLYFWRKTNNAASPSYCTWT
                     TGGFVSNGEAEVYDPNGVIQTGQGFFVEGTGSGTTVAFNNTMRTDNHANQFFRTSSEV
                     ERHRIWLNATGADGLFSQTMVGYMSDATNGYDATIDGKYINDGSIALTSLIDATPYAI
                     QGRSMPFDAADIVPMQFKAATAGTFSIAIDHVDGLFNGSQDIYLRDTQTGMDHDLKAS
                     AYTFSSEAGTFDTRFKLVYTAALSVDHPTLDANTIVIYKNENQSFTINSGAIAMASIK
                     VFDIRGRMITTQNNINTTQATITAGQTNQVLLVQVTSVDGATVTKKVVR"
     gene            complement(2055..2264)
                     /locus_tag="PK218_08880"
     CDS             complement(2055..2264)
                     /locus_tag="PK218_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98662.1"
                     /translation="MKNIFKLYLLTFFILSDFVVFAQPGSDDGSGGLEGDDPPAAPIN
                     SKLIVLLVLGLVFAFMQIKKYKKVS"
     gene            complement(2330..3919)
                     /locus_tag="PK218_08885"
     CDS             complement(2330..3919)
                     /locus_tag="PK218_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPW98663.1"
                     /translation="MSFLKEIERRRTFGIISHPDAGKTTLTEKLLLFGGAIQEAGAVK
                     NNKIKKGATSDFMEIERQRGISVATSVLAFIYKDKKVNILDTPGHKDFAEDTFRTLTA
                     VDSVIVVIDVAKGVEEQTEKLVEVCRMRKIPIIVFINKLDREGKDAFDLMDEVEQKLK
                     LTVTPLSFPIGMGYDFQGIYNIWEQNINLFSSDSRKNIEETVSFSDINNPELEKIVGE
                     KPANKLREELELISEVYPKFDRDAYLEGTLQPVFFGSALNNFGVRELLDCFIEIAPSP
                     RPKESETRLVKPEEEKMAGFVFKIHANMDPKHRDRLAFIKIVSGTFERNKPYMHVRLN
                     KNLKFSSPNAFFAEKKEIIDISYPGDIVGLHDTGNFKIGDTLTEGEMMSFKGIPSFSP
                     EHFRYINNADPMKAKQLDKGIDQLMDEGVAQLFTLEMNNRKVIGTVGALQYEVIQYRL
                     EHEYGAKCSYENFPVHKACWVKPNDPKSEEFREFKRIKQKFLAHDKYNQLVFLADSEF
                     TIQMTQSKFPNVKLFFTSEYD"
     gene            4165..6129
                     /locus_tag="PK218_08890"
     CDS             4165..6129
                     /locus_tag="PK218_08890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW98664.1"
                     /translation="MNSKKLFLGVALLVAFIANAQTKSKEVLFTINDKPYYTDEFSRV
                     YKKNLDLVKDESQKDLNLYLDLFIGYKLKVNKAYKLGLQDGQAYKNELKSYRTQLAKN
                     YFNDTKITEELIEEGYNRLQKEIKASHILILVDENAAPQDTLKAYNKIKDIRNRIVKG
                     EKFEDLAAQLSEDPSAKENKGDLGYFTAFRMVYPFENAAFTTPKGEVSKIIRTRFGYH
                     ILKINDIRPNRGEVTVAHIMILNPKPEDEDQNKAENTINDIYKKINQGEKFEELAKQF
                     SEDKSSASKGGVLNKFSSGQLSSEEFENIAFSLKNAGDISKPFKSQFGWHIVKLIEKH
                     PVRTLEEMKPELETKIGKDERSKRIVESLQEKLRKKYKFKKETKQLATISKLITNDYY
                     ESKWQMPTTGFDGKATILTIENKKIETNAFLEYLEKQQKSASPIKPINKLVDTMYDKF
                     VDEQLNTYYDENLETEFPEFSYVMEEYRDGLLLFDLMEKEIWNRSKTDTVGLENFYKA
                     NKDKHLWKKRIDATIFSSTKKDMIEKAISMLKKGAKPQEIKDKLNTKDVVNIMINTNT
                     FEEGNETLPKGTKFEVGTSSITKDGEYYFATKVDKVMPEGVKTLEECRGKLTNEYQQY
                     LEQHWVEDLKKEFNVSVNQAVFEKVKTELK"
     gene            6133..6981
                     /locus_tag="PK218_08895"
     CDS             6133..6981
                     /locus_tag="PK218_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98665.1"
                     /translation="MNKLFLILLCLSLGSCNWFKEEQKEDSIARVGKSYLYKEDIQNL
                     VPKGASKEDSILIIRSYIDRWATQKLLIEAAERNLGEAKKKEYATLISQYKIDLYTKA
                     YIEEIVKRSVDTLINNDELKKYYDENKENFKTNGSLVRLRYINLSKDNPKLETIKGKF
                     FNFDKKDKKFWDLNSMQFKSFAFNDSVWVDMGQVYEKLPIINPDNRDELIQSGKKIQV
                     LDKNDLYLVKISNVIAKNELSPFEYIKPTLKEIIINKRKLELIKKFEKEITEDAIKNK
                     DYEIYK"
     gene            6944..8368
                     /locus_tag="PK218_08900"
     CDS             6944..8368
                     /locus_tag="PK218_08900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW98666.1"
                     /translation="MPLKTKIMKFINSRFILTMVVAICSICSINSQEIIKDSVKPKAK
                     SVLPKKIKIDGIIATVGDYNILDSDIDKTYLELSSQGYSVKDITRCQMLSNLLDDKLY
                     AHQAVQDSITIGDGEVKEKMGEQIAYMVEQLGSMDKVLQYFKKDNEDEFRAELFDIIK
                     TNKLTAEMKRKIVDGVEITPEETRTFFKSIPTKDLPVFGAEMEVAQIVVAPKVTEEEK
                     QKVIKRLKEIKAEVLAGASFKTRAVIYTDDRASAPSGGFYKINRKTQFVKEFKDVAFS
                     LAEGEISEPFETEYGYHIIMVEKIKGQEVELRHILMIPKVSAEALKEAKEKITLIKKR
                     IEDGEITFADAARTMSDEKETRANGGVLINPQTQDTHFELTKMDPTLYSEVSNLKDGA
                     ITQPILDDDPRAGKKYKIITVTNRINEHTADYAGDYTKIKDLALKEKQIKAIAKWTKE
                     KIQETYIKINADYKDCSFANNWVK"
     gene            8410..>9296
                     /locus_tag="PK218_08905"
     CDS             8410..>9296
                     /locus_tag="PK218_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPW98667.1"
                     /translation="MSDVTAIQNLVQRRIELKKEVSKIIIGQDEVVDQIILSIFSGGH
                     ALLVGVPGLAKTLMVNTISQALGLEFKRIQFTPDLMPSDILGSEILDENRQFKFIKGP
                     IFSNIILADEINRTPPKTQAALLEAMQERSVTIAGQHYKLALPYFVLATQNPIEQEGT
                     YPLPEAQLDRFMFAIKLDYPSFQEEVQVVKSTTSDEKQTINALFTAQEIIDFQHLIRR
                     IPVADNVIEYAVTLVGKTRPDSDLSNDFVKNYLDWGAGPRASQNLILAAKANAAFNGK
                     FSPDIEDVKAVATGILRHR"
BASE COUNT         3357 a         1463 c         1653 g         2823 t
ORIGIN      
        1 acaggtacct attatataca atggattatg accgctagat ctggtggtag tttctatttt
       61 gatgatgttt cgattgtttc aggaacacca gaagttaaaa ccccaattgc tggttcacct
      121 tttacaataa atgccgcttc aactttatat aacgttacag gattaaatcc agtaactgca
      181 tatcattatg tagttagagc agtgcttggt gctgttattt caccaaatag caatgaaatt
      241 aatgttacaa ctactcttgg tacctgtact tgggatggtt cagcatggtc taacactact
      301 ggtcctactg tttctatagc agctattatt gaaggtgctt atgatactag tacttatgga
      361 ggatttactg ctaaagctgt tactataaac acaggtggtt ctttgactgt aaattcaggt
      421 gatactgtta cgatagttaa tagtttaaca aattcactta cggcagcagc tgtagtagtt
      481 gagaacaacg ctaatttact acaaaacgga agttcaaacc agaattcagg atcaatcact
      541 gttaaacgat atagttcagc attgaaacgt caagattaca cattgtggag ttcgccagta
      601 gctggacaac agttacagtc tttttcgcca ggcactttgt cgaatcgttt ttatacttat
      661 gatacttcgg cagatcaata tgctgcagtt gctacaccat ctgctactaa ttttgcaact
      721 gctacgggtt atttgataag aatgccaaat gtttatccaa ctacagctac tgtttggaca
      781 gatggtgctt ttgtgggtgt tccaaatagt ggtgattata actttacaat ggttgacggc
      841 ggttcaggat tgcgttttaa tttggttgga aatccttatc catcaccgat tgatgcagca
      901 gactttgttg gaaacactac caatgcaact aatactacag gaacattata tttttggaga
      961 aaaaccaata atgccgctag tccaagttat tgtacatgga caacaggagg atttgtttca
     1021 aatggagaag cagaagttta tgatccaaat ggagttattc aaacaggaca aggtttcttt
     1081 gtagaaggaa ctggaagcgg taccacagtt gcatttaata acacaatgcg tacggataat
     1141 catgctaatc aattctttag aacctcaagt gaagttgaac gtcatagaat ttggttaaat
     1201 gctactggag ctgacggatt gttttcacaa acaatggttg gttatatgag cgatgccact
     1261 aatggatatg atgccactat cgatggaaaa tatataaacg atggatctat tgcattaacg
     1321 tcattgatag acgctacgcc atatgctatc caaggaagaa gcatgccatt tgacgctgcg
     1381 gatattgtac caatgcaatt taaggcagca acagcaggaa cttttagtat cgctatagac
     1441 catgtagatg gattgtttaa tggatctcaa gatatttatt tgagagatac tcaaacagga
     1501 atggatcatg atttaaaagc aagtgcttat acatttagta gcgaagcagg aacatttgat
     1561 actcgtttta aattagttta tacagcagct ttatcagttg atcatccaac attggatgcc
     1621 aatacaatag ttatctataa aaatgaaaac caaagtttta caatcaactc tggagctatt
     1681 gcaatggcaa gtataaaagt gtttgatatt agaggaagaa tgataactac tcaaaacaat
     1741 atcaatacaa ctcaagctac tataacagca ggtcaaacta atcaagtact attagttcaa
     1801 gtcacttctg ttgatggtgc aacagttact aagaaagtgg ttaggtagat taaaaataca
     1861 ctaaaagtaa tataagtaaa aaagccgtct aaaatttgtt agacggtttt tttagctcta
     1921 cttttttgca taaaatcaat tttcaccaac tttttttgac aattaaacct aatttgttat
     1981 aattcaaaac aaccataaaa aatctcgcta ataattcaat tattagcggg atattttata
     2041 gattaaaaag ttaactaaga aacttttttg tattttttaa tttgcatgaa tgcgaaaact
     2101 aaacctaaaa ccaataatac aatcagctta gaattgattg gtgctgctgg tggatcgtct
     2161 ccttctagac ctccactgcc atcatcagat cctggctgag caaaaacaac gaagtctgat
     2221 aaaataaaaa aagttaaaag gtataattta aaaatgtttt tcatgtcatt atattttcaa
     2281 gttggtaaag atagtaatca tttttgtact ttaaaaattt tatgttaaat taatcgtatt
     2341 cagaggtaaa gaaaagttta acatttggaa attttgattg tgtcatctga atagtaaact
     2401 cagaatcggc taaaaatacc aattgattgt atttatcgtg ggctaagaat ttttgcttga
     2461 ttctcttgaa ttccctaaat tcttcacttt taggatcgtt tggttttacc caacaagctt
     2521 tgtgaacagg aaaattttca taactacatt tagcaccgta ctcatgctct aaacgatatt
     2581 gaattacctc gtattgtaat gcacctactg tcccaattac ttttcgattg ttcatttcta
     2641 gtgtaaataa ttgagcaaca ccttcgtcca ttaattgatc aatacctttg tccaattgtt
     2701 ttgctttcat tgggtcggca ttgttgatgt atctaaaatg ttcaggagaa aagctaggaa
     2761 ttcctttgaa actcatcatt tcgccttcgg ttaatgtatc accaatttta aaattacctg
     2821 tatcgtgtaa tcctacaatg tcaccaggat aggaaatgtc tataatttct tttttctcag
     2881 caaaaaaggc atttggactc gaaaatttta agtttttatt taatctaaca tgcatgtaag
     2941 gcttatttct ttcgaaagta cccgaaacta ttttgataaa tgccaaacgg tctcggtgtt
     3001 taggatccat attagcatgg attttaaata cgaaacccgc cattttttct tcttcaggtt
     3061 ttactaaccg cgtttcggat tcttttggtc tcgggcttgg agctatttcg atgaaacaat
     3121 ctagtaattc gcgaacacca aaattgttta aagcagaacc aaaaaaaaca ggttgaagag
     3181 ttccttctaa ataggcatct ctatcgaatt tgggatacac ttcagaaatt aactctaatt
     3241 cttctctgag tttattggct ggtttttcgc caactatttt ttctaattca ggattgttga
     3301 tgtcgctaaa tgataccgtt tcttcaatat tttttcggct gtcactactg aacagattta
     3361 tgttttgttc ccagatatta taaattcctt gaaaatcgta tcccattcca ataggaaaac
     3421 ttaaaggtgt aacggttagc tttagttttt gttctacttc gtccataagg tcaaaagcat
     3481 ctttaccttc acgatcaagt ttgttaatga aaacaataat aggaatcttt ctcattctac
     3541 aaacttcgac tagcttttct gtttgttcct caaccccttt tgcaacgtca ataactacaa
     3601 taacactatc aacagccgta agtgttcgga aggtatcttc ggcaaaatct ttgtgacctg
     3661 gtgtgtcaag aatattcact tttttatcct tataaataaa agccaaaaca gaagttgcta
     3721 ccgagattcc acgttgacgt tcaatctcca taaaatcgga agttgctcct ttttttatct
     3781 tattattttt aacggcacct gcttcttgaa tagcaccacc aaaaagcagt aacttctctg
     3841 ttaaagttgt ttttccagca tcaggatgcg atatgattcc aaaagttctg cgacgttcta
     3901 tttcttttaa aaagctcatg ttttattcta aattggatgc aaaaatacta tttatttttt
     3961 catagactac tattcggtta tactgttgcc taaagttgat tcaaaaagtg ttaataaaat
     4021 tttgttaata ctaaagttga ttcttgttta atctgattga attgttactt ttgttgattg
     4081 gtatgttgtc atattgattt tttggttgca tacaacagaa aagtttaata atttgtttca
     4141 ttttttaagt tataataata agtaatgaat agtaaaaagt tgtttttagg cgtagcattg
     4201 ctggttgctt ttatagcaaa tgctcaaaca aaatcaaaag aagttttgtt tacaataaat
     4261 gacaaaccct attacaccga tgagttttca agggtttata agaagaattt agatttggta
     4321 aaagacgaat cgcagaagga tttaaacttg tatttggatt tgtttattgg ttataaatta
     4381 aaagtaaata aagcttacaa actaggattg caagatgggc aagcgtataa aaatgagttg
     4441 aaatcgtata gaacccaatt ggccaaaaac tattttaacg atactaaaat aactgaagaa
     4501 cttattgaag aaggttataa tcgactacaa aaagaaataa aagcttctca catacttatt
     4561 ttggttgatg agaatgccgc tccacaagac acattgaaag cttacaataa aataaaagat
     4621 atcagaaacc gtattgttaa aggcgaaaaa tttgaagatt tagcggcaca gctttcggaa
     4681 gatccatcgg ctaaagaaaa taaaggtgat ctaggttatt ttactgcttt tagaatggtt
     4741 tatccttttg aaaacgctgc ttttactact ccaaaaggag aagtttctaa gataataaga
     4801 actcgatttg gatatcacat acttaaaatt aacgatatta gaccaaatag aggtgaagtt
     4861 accgtggcgc atatcatgat tttaaatcca aaaccagaag atgaagacca aaataaggca
     4921 gaaaacacaa tcaatgatat ttacaaaaaa ataaatcaag gcgaaaagtt tgaagaattg
     4981 gcaaaacaat tctctgaaga caaatcttct gcgtcaaaag gtggtgtttt gaataagttt
     5041 agttcgggac aattgagttc ggaagaattt gaaaatatag ctttttcttt aaaaaatgcg
     5101 ggtgatattt caaaaccatt caaatcacaa tttggttggc atattgttaa attgattgag
     5161 aaacatccag tgcgaacatt ggaggaaatg aagccagaac tagagacaaa aatcgggaaa
     5221 gacgaacgtt ctaaaaggat tgtagaatca ttgcaagaaa agcttagaaa aaaatacaag
     5281 ttcaaaaaag aaaccaaaca attggctaca atttctaagt tgataactaa tgattattat
     5341 gagtcgaaat ggcaaatgcc gactacaggt tttgacggaa aagctacaat attgactatt
     5401 gaaaataaaa agatagaaac taatgccttc ttagaatatc ttgaaaaaca acaaaaatca
     5461 gccagtccaa taaaaccaat caataaattg gttgatacta tgtatgacaa gtttgttgac
     5521 gaacaattaa atacatatta cgatgagaat ttagaaacag agtttcctga gttttcttat
     5581 gtaatggaag aatatcgcga tggattattg ctttttgatt tgatggaaaa agaaatttgg
     5641 aatcgttcaa aaacagatac tgttggacta gagaatttct ataaagcgaa taaagacaaa
     5701 catctttgga aaaaaagaat tgatgcaacc attttttctt ccaccaagaa agacatgatt
     5761 gaaaaagcca tttcgatgct taaaaaaggt gctaaaccac aagaaataaa agataaactc
     5821 aatacgaaag atgttgttaa tattatgatt aacaccaata cttttgaaga aggaaatgaa
     5881 acattgccaa aaggaacaaa atttgaagta ggaacttctt caattacgaa agacggcgag
     5941 tattattttg ctacgaaagt agataaagtt atgccagaag gcgtaaaaac attagaggaa
     6001 tgtagaggta aattgaccaa tgaataccaa caatatttag agcaacattg ggttgaagat
     6061 ttgaaaaaag agtttaacgt ttcggtaaat caagctgttt ttgaaaaggt taaaactgaa
     6121 ttaaaataat agatgaacaa actttttttg atactacttt gtctttcact tggctcatgt
     6181 aattggttta aagaagagca gaaggaagat tcgattgcac gtgttgggaa aagttattta
     6241 tacaaagagg atattcagaa tttagtgcca aaaggcgcat caaaggaaga tagtatttta
     6301 atcatcagaa gttatataga ccgatgggca actcaaaaat tattgattga agccgcagaa
     6361 agaaatttgg gtgaagccaa gaaaaaagag tatgctactt tgataagcca gtacaaaatt
     6421 gacttataca ccaaagccta tattgaagaa attgtaaagc gttccgtaga tactttaatc
     6481 aataatgatg aattgaaaaa gtattatgat gaaaataaag agaactttaa aaccaatggt
     6541 agcttagtta gattgcgata tatcaatttg tccaaagaca acccaaagtt agaaactatt
     6601 aaagggaagt tttttaattt tgataaaaaa gacaaaaagt tttgggattt aaactcgatg
     6661 cagttcaaaa gttttgcttt taatgacagc gtttgggtgg acatgggaca agtctatgaa
     6721 aaattgccta tcatcaaccc agacaataga gatgaactaa ttcagtcggg aaaaaaaata
     6781 caagtattgg ataaaaatga tttgtattta gttaaaataa gcaatgtgat tgcaaaaaat
     6841 gagttgtcac cttttgaata tataaagcca accttaaagg aaataataat aaacaaacga
     6901 aaactcgaat taattaaaaa gtttgaaaaa gaaattacag aagatgccat taaaaacaaa
     6961 gattatgaaa tttataaata gccgatttat actaacaatg gttgtagcaa tttgttctat
     7021 ttgctccata aatagtcaag aaatcattaa agactcggtg aaacctaagg cgaaaagtgt
     7081 gttgcctaaa aagattaaaa tagacggaat tattgccacc gttggcgatt ataatatttt
     7141 agattctgat attgataaaa cttacttaga actttcaagt caaggttatt cagttaaaga
     7201 tattacccgt tgccaaatgc tttctaatct tttagacgat aaattgtatg cccatcaggc
     7261 agtacaagac agtattacaa ttggagatgg agaagtaaaa gaaaaaatgg gcgaacaaat
     7321 tgcttacatg gtagaacaat taggctctat ggataaagtg ttgcaatact ttaagaaaga
     7381 taacgaagac gagttcagag cagagctttt tgacattatt aaaaccaaca agctaacggc
     7441 agaaatgaaa cgaaaaattg tagatggagt tgaaattaca cccgaagaaa cacgtacttt
     7501 tttcaaatcc attccaacca aagatttacc cgtttttggt gccgaaatgg aagtagcaca
     7561 aattgtcgtt gcgccaaaag tcaccgaaga agaaaagcaa aaagtcatca agcgactcaa
     7621 ggaaatcaaa gccgaagtat tggcaggagc cagttttaaa actcgagccg ttatttatac
     7681 cgacgataga gcctcggcac catcgggagg tttttataaa ataaatagaa aaacccaatt
     7741 cgtaaaagag tttaaagacg tggcttttag tctcgccgaa ggtgaaatat cagaaccatt
     7801 cgaaacagaa tatggttatc atatcattat ggttgaaaaa attaaaggtc aagaagtaga
     7861 attaagacac atcttgatga taccaaaagt ctctgcagaa gcattaaaag aagctaaaga
     7921 gaaaatcacg ttaattaaga aaaggattga agatggcgaa ataacttttg ctgatgctgc
     7981 aagaacgatg tctgacgaaa aagaaacaag agccaatggc ggtgtgttaa ttaatccaca
     8041 aacccaagat acgcattttg aattaactaa aatggatcca acactataca gtgaggtgtc
     8101 taatttaaaa gacggagcta tcacacaacc cattttggat gatgacccaa gagcaggaaa
     8161 aaaatacaaa atcattaccg taacaaacag aattaacgaa catacagctg attatgctgg
     8221 agattatacc aaaataaaag atttagcctt aaaagaaaag caaatcaaag ccattgctaa
     8281 atggaccaaa gaaaaaattc aagaaacgta tattaaaata aatgcagatt ataaagattg
     8341 tagcttcgcc aataattggg taaagtaaca gcttatttat tctttgatat ttaaccaaaa
     8401 ccattccgaa tgtctgacgt aaccgcaata caaaatttag ttcaacgaag aatcgaactc
     8461 aaaaaagaag taagtaaaat tatcattggt caagacgaag tagtcgatca aattatttta
     8521 agtatttttt cgggtggtca tgcactatta gtgggtgttc caggattggc aaaaacatta
     8581 atggtcaata ccatttcgca agcgttagga ttagaattca agcgtattca attcacaccc
     8641 gatttgatgc cgtcggatat attaggaagt gaaattttag atgaaaaccg tcagttcaaa
     8701 ttcatcaaag gaccaatatt ttctaacatc atccttgccg atgaaattaa tagaacacca
     8761 ccaaaaacgc aagcggcatt actagaagcc atgcaagaac gttcggttac tattgcgggt
     8821 caacattaca aactagcatt gccttatttc gttttggcaa cacaaaaccc tattgagcaa
     8881 gaaggaacct atccgttacc cgaagcacaa ttagaccgtt ttatgtttgc gattaaacta
     8941 gattatccat cttttcaaga agaagtacag gtggtaaaaa gcaccacttc cgatgaaaaa
     9001 caaaccataa atgctttatt tacggcgcaa gaaatcatcg atttccaaca tcttattcgt
     9061 cgtattcctg ttgcagacaa cgttatagaa tatgccgtaa cattagtagg caaaacacgt
     9121 cctgatagcg atttgtctaa tgattttgtt aaaaactatt tagattgggg cgcaggacca
     9181 agagcatcac aaaacttaat ccttgctgcc aaagccaatg cagcttttaa tggaaagttc
     9241 tcgccagaca ttgaagacgt aaaagctgta gccactggaa ttcttcgtca cagaat
//
LOCUS       DAOOBH010000090         9294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_9198_length_9294_cov_5.214958, whole genome shotgun sequence.
ACCESSION   DAOOBH010000090 DAOOBH010000000
VERSION     DAOOBH010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9198_length_9294_cov_5.214958"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1394)
                     /locus_tag="PK218_08910"
     CDS             complement(141..1394)
                     /locus_tag="PK218_08910"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPW98668.1"
                     /translation="MNVIIRNAKVIDENSTFHNQTVSLKIKNGTIEAIAASIKEESGY
                     KEIEISGLHVSQGWFDSSVSLGEPGFEDREIIKNGLEVAAKSGFTGIALQPNTFPVLD
                     NQSQINFVKQKATNAVTDLFPIGALTKNSEATDLAELFDMKNAGAIAFGDYNKSISNA
                     NLLKIALQYVQDFDGLVIAFCQNQNLKGNGVANEGITSTRLGLKGIPSLAEELDVARN
                     LFLLEYTGGKMHIPTVSTAKSVALIKEAKAKGLNVTCSVAVHHLTVTDEKLESFDSRY
                     KVAPPLRTEIDRKALIKGILNDTIDCITSDHNPIDIEQKKMEFDMAKSGTIGLESAFG
                     ALNTVLPIEVIIKKLTAAKALFQIENQSIEIGNKANLTFFTTDNNWHFTKENILSKSK
                     NSAFLGQKMKGKVVGVYNNKTLILA"
     gene            complement(1391..3313)
                     /locus_tag="PK218_08915"
     CDS             complement(1391..3313)
                     /locus_tag="PK218_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HPW98669.1"
                     /translation="MQFKHPEFLYFLFLLVIPILVHLFQLRRFKREYFTNVQFLKELA
                     VQTRKSSTIKKWLLLFTRLLLLTFLILAFAQPFFSAKESPKANNEMYIVLDNSYSMQA
                     KGKQGELLKRAVQDLLEHTPENQSFSLMTNSENYWNTDIKSIQKELQNLDYSATGFEI
                     ESAIAKINARKSAFNKDIIVITDAIGIKNNQTKGFKKEDNVYFIIPEAEQKNNVAVDS
                     VYLTQTLDNFYEIGVSLSSYGINKTETPVALYNKGKLIAKTLLSIENGKKSINFTIPK
                     EEFNGYVSIEDKGLSYDNTYYFSITKSRKTNVISIGDSEKSNFLARIYTSDEFNYSNF
                     PLNSLDYNQIEKQDAIIINELVTIPQALQTTLKNFVSKGGNVIFIPSNENTNANSFLA
                     NFGAIQLQSATTNEKKVTKINFNHPLFNSVFEKKVDNFQYPMVKNSFSINSKTPSILS
                     YEDQSTFLTSIKNDLSSVYVFAAPINKSNSNFQNSPLIVPIFYNMSQNALRLGISDIK
                     IGSNQPFVVETTMGKDEIVELKNDIERFIPSQQLMASKVKMTFNENPKNAGNFGIFNG
                     NNLIENLGFNYNRSESNLSQSNSNIADDFSLINDVDSVFNTIQIDRTDTQIWKWFVML
                     ALLFLVIELLIQKFVK"
     gene            complement(3428..4774)
                     /locus_tag="PK218_08920"
     CDS             complement(3428..4774)
                     /locus_tag="PK218_08920"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW98670.1"
                     /translation="MSTFEQFNLPKSLQKAIDELGLTTPTPIQEKSFSIIMSGRDVMG
                     IAQTGTGKTYAYLLPILKQWKFIATDTPRVLILVPTRELVVQVAAEVEKLTQYMSVRT
                     LGVYGGVNINTQKKAVYQGVDVLVGTPGRIMDLALDNVIRFDSMQKLIIDEFDEMLNL
                     GFRYQVTSILTMLKNKKQSILFSATMTDQVDEMLDEYFEFPEEVSLAPSGTPLEKIEQ
                     FYYKVPNFLTKINLILELLKDESLERVLLFVNNKKTADLLAEKVEEIYPEQFGVIHSN
                     KSQNYRLQTMAQFQEGSIRGIVTTDVMARGLDISDISHVINIEFTDIPEKYIHRIGRT
                     GRADKSGIAISLIAPVEEEIMIEAEVLMEKEITFLPFPEGVEVAERLLEFEKDKKQMK
                     FLLKKPKLEGGASFHEKKDKNKKVNLGGPSKTKKKTGTSVNRNITKTRAAKRKNKK"
     gene            complement(4901..6361)
                     /locus_tag="PK218_08925"
     CDS             complement(4901..6361)
                     /locus_tag="PK218_08925"
                     /EC_number="1.14.13.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPW98671.1"
                     /translation="MNKAENKTNWTTCPECKGRGKKSQKLRKKERIRYQIALTEFENN
                     NKQGTAPIPPKAHLSSCSNCSASGLILATHPTIPDTENYPHLAIIGGGIGGIALAVAC
                     LHRGIPFTLFERDTSFDARAQGYGLTLQQASKAIQGFGIMSLKEAVISTRHVVHTTDG
                     NIIGEWGIRKWMPAVTKTTPKRTNVHIARQSLRKALLEQLGGKKAIQWGHQLVAIKVA
                     EDNTVTLNFHVDGTIKTTKADIVIGADGIRSTVRKLLIGDEVSPLRYLDCIVILGICP
                     LTALEGVESPLLDAATVFQTANGNERIYMMPYDSDSVMWQLSFPMYEKAAKTLSKKGV
                     QALKEEAFRRTQWHNPIPQIIAATKATQITGYPVYDRELLCPELLAKNEYGTLIGDAA
                     HPMSPFKGQGANQALLDALSLARTITKECRPLSNWRENGIRKTILTNFETEMLRRSAT
                     KVKDSAEAAQFLHSDVALYEGDEPRGRVLKRKENRE"
     gene            complement(6420..6608)
                     /locus_tag="PK218_08930"
     CDS             complement(6420..6608)
                     /locus_tag="PK218_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98672.1"
                     /translation="MNKRLYITLTILFGFISFGATSETHRIMTYSSIDFAPQRMYLTN
                     LALMFLISLLLLTRYFCY"
     gene            complement(6676..7797)
                     /locus_tag="PK218_08935"
     CDS             complement(6676..7797)
                     /locus_tag="PK218_08935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPW98673.1"
                     /translation="MTKSKLNQIGILIAFSIVLSSCKLGRFVFYNFADIKDYKIFPSR
                     TLVSNQTKFEFYATDEGKYPKTLKFSNNIEPVTFDKVLEDNKTVAFLIIKNDTIQYEK
                     YFKGYDKESIIPSFSMAKSVTSILIGCAIDDGLIKSVDEPITNYIPELKKNGFETVTL
                     KHLLQMTSGLDFNESYFNPFGEAASFYYGRNLKREVSKLKLKRKPGEKFEYASGNTQL
                     LGSVLQSVLKDKTITQYFQEKLWTPLEMEYDASWSIDKKKEGTEKTFCCINARARDFA
                     KIGRLYLNKGNWNGKQIISQKWVEESTKVDTTEGSDEGYQYQWWLPSNTGEYMAEGIL
                     GQFIYVNPAKNIVIVRMGKNYGDINWSDLFSLLAKYPDK"
     gene            complement(8046..9128)
                     /locus_tag="PK218_08940"
     CDS             complement(8046..9128)
                     /locus_tag="PK218_08940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HPW98674.1"
                     /translation="MIDTTRRIQKLKTELFFDISPDLFFIAGYDGYFKKVNPAVCNLF
                     GYSEEELLAKPINDFIHKDDIEKTIKSREKVISGNTLMHYENRYITKSGEIVWLAWTS
                     VPLMDENLVYAIAKDVTYKKRIEEERNLLLASLKKINNNLKNLTYTASHDMRSPVNNL
                     LTVFDLLDTSKIQDEETLEFINVLKTVSEGLKDTLNNYVDNINHNDKMSLSVDRLAFQ
                     PSLDNVLLSIKSLIKESSTSFEIDFSQAETVSFNKAYLESIFINLITNAIKYSRPEVP
                     PMITIKTKKVNGVTSLLFSDNGLGFDINKIENRIFKLNQKFHTNKDSKGIGLYLIYNH
                     VKDLGGHIEMTSQLNKGTTFTITFKE"
BASE COUNT         2647 a         1527 c         1582 g         3538 t
ORIGIN      
        1 cgagcgattt ccgaaaagaa gcaaattctg atttgttttc atcttgattc atgaaaatgg
       61 caactactga tccaatgatg gtcatatagg ctactatggc ggtattcttt cctttatctg
      121 taaaattttc cataattgat ttatgctaaa attaatgttt tgttgttata aaccccaact
      181 acttttcctt tcatcttttg acctaaaaat gcagaatttt tagatttcga taaaatattt
      241 tcttttgtaa aatgccaatt attgtcagta gtaaaaaatg ttaaattggc tttatttcct
      301 atttcaatgg attggttttc tatttgaaac aaagcttttg cagcggttaa ttttttaata
      361 attacctcga taggtaagac agtgttcaat gctccaaaag cactttctaa accgatagta
      421 ccgctttttg ccatatcaaa ttccatcttc ttctgttcaa tatctattgg attatggtct
      481 gaagtaatac aatcaatagt gtcgtttaaa atgcctttaa tcaaagcttt tcgatctatt
      541 tctgttcgta atggaggtgc cactttatat cgtgaatcaa aactttctag tttttcgtct
      601 gttactgtta aatgatgaac agcaacactg catgtaacgt ttaatccttt tgcttttgct
      661 tctttgatta atgcaaccga ttttgcagtt gatacggttg gaatgtgcat tttaccacca
      721 gtatattcta ataaaaacaa atttctagcg acatctaatt cttcggctaa acttggaatt
      781 ccttttaatc ccaatcgggt agaagtgata ccttcattgg caacaccatt tccttttagg
      841 ttttgatttt gacaaaaagc aatcactaag ccatcaaaat cttgaacgta ctgtaaagct
      901 attttgagca aattggcatt agaaatactt ttattataat caccaaatgc aatggctcca
      961 gcatttttca tgtcaaatag ttcggctaaa tcggttgctt cactattttt agtcaatgca
     1021 ccgattggaa acaaatctgt aaccgcatta gtagcttttt gttttacgaa attaatttgt
     1081 gattgattat ctaaaacagg aaaagtattg ggttgcaatg ctattccagt gaagccactt
     1141 ttagcagcaa cttctaatcc attttttata atttctctgt cttcaaatcc tggttcacct
     1201 agtgagacac tgctgtcaaa ccaaccttgt gaaacatgaa gaccgctgat ttctatctct
     1261 ttataaccac tttcttcttt gatagatgca gctattgctt caatcgttcc gtttttaatt
     1321 tttagagaaa ccgtttgatt atgaaaagta ctattctcgt caattacttt tgcatttcta
     1381 ataattacat tcattttaca aatttttgga ttagtaattc aatcactaaa aagagtagcg
     1441 ctagcatcac aaaccatttc cagatttgtg tatcggttct atcgatttga atagtgttga
     1501 atacactatc aacatcattt attaaagaaa aatcgtctgc aatattacta ttactttgcg
     1561 ataaattact ttcacttctg ttgtaattaa aacctaggtt ttctattaaa ttatttccgt
     1621 tgaatatacc gaaattacca gcattttttg ggttttcatt gaaagtcatt tttactttgg
     1681 atgccatcaa ttgttgcgat ggaatgaatc tttcgatatc attctttagt tctacaattt
     1741 cgtctttacc catcgttgtt tctactacaa aaggttgatt gctgccaatt tttatatccg
     1801 atattcccaa gcgtagtgca ttttgtgaca tattataaaa tattggaaca atcagcggtg
     1861 aattttggaa attactattg cttttattga ttggtgctgc aaaaacatat actgaagata
     1921 agtcgttttt aatagaagtt agaaacgtac tttgatcttc atagcttaat atacttggcg
     1981 ttttactgtt tatactaaat gagtttttaa ccattggata ttgaaagtta tcaactttct
     2041 tttcaaatac tgaattgaat aatggatgat tgaaatttat tttggttact ttcttttcat
     2101 ttgtagtagc cgattgcaat tgtattgctc caaaattagc taagaaactg ttggcgtttg
     2161 tgttttcatt ggaaggaata aaaattacgt tcccaccttt tgacacgaaa ttttttaacg
     2221 ttgtttgcaa agcttgtgga atagttacca actcatttat tataatagcg tcttgctttt
     2281 caatttgatt gtaatccaag ctgtttaacg ggaagttgct gtaattgaac tcatcactag
     2341 tataaattcg tgctaaaaaa ttactttttt ctgaatctcc tatgctgata acattggttt
     2401 ttcttgattt tgtgatgcta aaataatagg tgttatcgta gctcaatcct ttatcctcaa
     2461 tggaaacata tccgttgaac tcttcttttg gaatggtgaa gttgatggat ttctttccat
     2521 tttctattga caataaggtt ttggctatta gtttaccttt gttgtataaa gctacaggtg
     2581 tttccgtttt attgatgcca taagacgaaa gcgatacgcc aatttcatag aaattatcta
     2641 aggtttgtgt caaataaacg ctatcaacag ccacattgtt tttctgttcc gcttcaggaa
     2701 tgatgaaata gacgttgtct tcttttttaa atcctttggt ttggttgttt tttatgccaa
     2761 tggcatcagt aatcacgatg atatctttgt tgaaggctga tttacgagca ttaattttcg
     2821 caatagcact ttcaatttca aaaccagtgg cgctataatc taaattttgc aattcttttt
     2881 gaatggattt tatgtcggta ttccaataat tttccgaatt ggtcattaaa gaaaagcttt
     2941 gattttcggg agtgtgttct aataaatctt ggacggcacg ctttaacaat tcaccttgtt
     3001 ttccttttgc ttgcatacta taggagttat ctaaaacaat atacatttcg ttgttagctt
     3061 tcgggctttc tttagcagaa aagaacggtt gtgcaaaggc tagaatcaag aaagtaagta
     3121 gtaataaacg agtaaatagg agtaaccact tttttatggt ggaacttttt cgagtttgta
     3181 ccgctagttc ttttaggaat tgtacgttgg taaagtattc tcttttaaaa cgacgtaatt
     3241 gaaataaatg taccagaatt gggatgacca ataagaataa gaagtataga aattctggat
     3301 gtttaaattg catttttact attgtttgtc aaaaatagca aatttctcag tagaaaaaag
     3361 aatatagagg atagaaaaaa gaaaaaagaa gtgtttgttt gagtctttca ctaataatta
     3421 gtaaaaacta ttttttattc tttctcttag cagctctagt tttggtgatg tttctattta
     3481 cagatgtacc cgttttcttt ttggtttttg atggtccgcc aaggtttact ttcttgttct
     3541 tgtctttctt ttcatggaat gaggcaccgc cttctagttt tggtttcttc aacaaaaact
     3601 tcatttgttt tttgtctttt tcaaattcca ataaacgttc ggcaacttca actccttctg
     3661 gaaaaggaag gaatgtgatt tctttttcca tcaatacttc tgcttcaatc atgatttctt
     3721 cttccacagg agcaattaaa ctgatagcaa tacccgattt gtcagcacga ccagttctac
     3781 caatacggtg aatgtacttt tcgggaatat cggtaaactc tatgttgata acgtgactga
     3841 tgtctgatat atccaaacct cttgccatta cgtcggtagt tacaattcct ctgatacttc
     3901 cttcttgaaa ctgcgccatg gtttgcaaac ggtaattctg agatttgttc gaatgaataa
     3961 ctccaaactg ctctggatat atttcttcta ctttctctgc caacaaatcg gccgttttct
     4021 tgttgtttac aaacaataaa acacgttcta acgattcgtc ttttagcaat tcaaggataa
     4081 ggttaatctt ggttaaaaag ttaggtactt tataataaaa ctgttctatt ttttctaaag
     4141 gtgttccact tggtgctaat gacacttctt caggaaattc aaagtattcg tctaacattt
     4201 catctacttg atccgtcatt gttgcggaga aaaggatgct ctgctttttg ttttttaaca
     4261 tcgtcaagat agatgttact tgatatcgga accctaaatt caacatttca tcaaactcgt
     4321 cgatgattag tttttgcata ctatcaaaac gaatgacgtt gtctaacgcc aaatccatga
     4381 tgcgtcctgg tgtgccaact aatacatcaa caccttgata gacagccttt ttttgcgtat
     4441 taatgtttac accaccataa actcctaggg ttctaacaga catgtattgt gttagttttt
     4501 ctacttcggc agctacttga accaccaatt cacgtgtggg tactaagatt aaaactcttg
     4561 gggtatctgt agcaataaat ttccattgtt ttaaaatggg taatagatag gcgtatgttt
     4621 ttcctgttcc ggtttgtgca attcccatca catctctacc cgacatgatt attgaaaatg
     4681 atttttcttg aattggagta ggtgtggtta accccaactc atcaatggct ttttgaagtg
     4741 atttgggtaa gttgaactgc tcgaaagtgg acatacaatt attaattttt gcaaaggtaa
     4801 ggatttaaaa ttgctttcga gttttgtgtt tgtataaagt aattggataa cactcaataa
     4861 gagtaacact gtttttttaa ctatctaagg tttttgttga ttactctcta ttttcttttc
     4921 gctttaaaac tcttcctctg ggttcatcac cttcatatag cgcaacgtcg gaatgaagaa
     4981 attgtgcagc ttcagcagaa tctttaactt tggtagcact tcttcttaac atttcagttt
     5041 caaagtttgt tagtatcgtt tttcttattc cattttctct ccagtttgat aatggtcggc
     5101 attcttttgt gattgttctt gccaatgata atgcgtctag tagtgcttgg ttggcacctt
     5161 gtcctttaaa tggactcatt ggatgagctg catcgccaat gagtgtgccg tattcatttt
     5221 ttgctaaaag ctcggggcag agaagttcac ggtcatacac gggatagcca gtaatttgtg
     5281 ttgctttggt agctgctatt atttgaggta ttggattgtg ccactgtgtt ctacggaacg
     5341 cttcttcttt taatgcttga actccttttt tgcttaatgt ttttgctgct ttttcataca
     5401 ttggaaagct tagttgccac attactgagt cagaatcata aggcatcatg tagatgcgtt
     5461 cattgccatt ggcggtttga aatactgttg ctgcgtcaag taaaggactt tctacacctt
     5521 cgagtgctgt taatgggcaa atacctaata tgacgataca atcgaggtaa cgcaaagggg
     5581 aaacttcatc accaatgagt aactttcgaa cagtactacg aataccatca gcaccaatta
     5641 caatgtcggc ttttgtggtt tttattgttc catctacatg aaagtttagt gtaacggtgt
     5701 tgtcttcagc tacttttata gctactaatt ggtgtcccca ttgtatggct ttttttcctc
     5761 caagttgctc aagtaatgct ttacgcagcg attgtcgtgc tatgtgaaca ttggtacgtt
     5821 tgggagttgt ttttgtgacg gctggcatcc attttctaat tccccattcg ccaataatat
     5881 ttccatctgt tgtatgtact acatgtcttg ttgaaatgac agcttctttt aatgacatga
     5941 tgccaaatcc ttgtatggct ttactagctt gttgcaaggt gagaccatag ccttgtgctc
     6001 ttgcatcaaa actagtatcg cgctcaaaaa gtgtaaatgg aattccgcga tgtaaacagg
     6061 ctacggctag tgctatacca ccaataccac caccaataat ggctaaatgc ggatagtttt
     6121 cggtatcggg aatagttgga tgagttgcca gaattaagcc cgaagcagaa caatttgagc
     6181 aggaggaaag atgtgctttt ggaggaatgg gtgcagtgcc ttgtttgttg ttattttcaa
     6241 attcggttag agcaatttga tagcggattc gctctttctt gcggagcttt tggctttttt
     6301 tacctcttcc tttgcattcg gggcatgtag tccagtttgt tttattttcc gctttattca
     6361 cttgtttgtt taatataatt ttgacttatc gtgactgatt acaccaacta tctttttgtt
     6421 taataacaaa aatagcgtgt caaaagtagt aaacttatta aaaacatcaa tgccaagttg
     6481 gttagataca ttctttgtgg agcaaaatcg atacttgagt aggtcattat tctatgagtt
     6541 tcagatgttg cgccaaatga tatgaatccg aatagaatgg ttagtgttat gtatagtctt
     6601 ttattcatgg atttttttaa aattacagtg catactattc taatgcttcc aatgatgtta
     6661 aaactaataa taattttatt tgtcgggata ttttgccaat aaactaaaaa gatctgacca
     6721 attaatatct ccataatttt tacccattct cacaattaca atgtttttgg ctggatttac
     6781 atagataaat tggcccaata ttccttcagc catatattct cctgtatttg acggtaacca
     6841 ccattgatat tgatagcctt catcacttcc ttctgtggta tcaacttttg tagattcttc
     6901 tacccatttt tgagaaatta tttgcttacc attccagttt cctttgttta aatagagtcg
     6961 tccaattttg gcaaaatctc ttgctcttgc attaatacaa cagaatgttt tttcggttcc
     7021 ttcctttttt ttatcaatgc tccatgaagc atcatattcc atttcgagtg gagtccatag
     7081 tttttcttga aaatattgtg taattgtttt atcttttaaa acactttgta aaactgaacc
     7141 aagtagttga gtatttccgc tggcgtattc gaacttttct cctggttttc tttttaattt
     7201 taatttagaa acttcacgtt ttaggtttct accataatag aatgaggctg cttctccaaa
     7261 tggattaaaa taactttcat taaagtctaa tccagatgtc atttgtagca aatgttttag
     7321 agttactgtt tcaaatccat ttttttttag ttcgggaata taatttgtta ttggttcatc
     7381 gaccgatttt attaaaccat catctatggc gcaacctata agtatggatg ttactgattt
     7441 tgccattgaa aaagaaggga taatactttc tttatcatat cctttaaaat atttttcgta
     7501 ttgaatggta tcatttttga ttattagaaa tgctactgtt ttattgtctt cgagaacttt
     7561 atcgaaagtt acaggttcta tattgttact aaattttaat gtttttggat attttccttc
     7621 atcagttgcg tagaattcga atttggtttg attactaact agagttcgtg acgggaatat
     7681 tttatagtct ttaatatcgg caaagttata aaagacaaat cgaccaagtt tgcaagatga
     7741 taacacaatt gaaaaagcaa ttagaattcc aatttgattt aattttgatt tagtcatgtt
     7801 taattttttt tagcttgact ttataagtga ggttttatat tccctaaaat aattaaaaat
     7861 caatctgttt cagaaaatga tttataataa gatggtttat tttgcagggt tataattttt
     7921 taatgccgat attgaaatgc ttagtaccat atgacttgag tctatgatat gcaattgagt
     7981 tttggatgat ttacttcatg cgaccaccct tcgtcaaggt aacaaagtga atgtgaggtt
     8041 tgaagttatt ctttaaaagt tattgtaaaa gtggttcctt tattaagttg gctcgtcatt
     8101 tcgatatgtc ctcctaaatc ttttacgtgg ttgtaaataa ggtatagccc aattcctttg
     8161 ctgtctttgt ttgtatggaa cttttgattg agtttgaaga ttctattttc aattttattg
     8221 atgtcaaatc ctagcccatt atcagaaaat agtaatgagg taactccatt aacttttttg
     8281 gttttaatgg taatcattgg cggcacttca ggacgagagt attttattgc gttggttatt
     8341 aagttgatga aaatactttc aagatatgcc ttgttgaacg aaactgtttc agcttgcgag
     8401 aaatcaattt caaatgatgt actcgattct ttaattaatg atttaattga taacaaaaca
     8461 ttatcaagtg atggttgaaa ggctaaccta tctaccgata gactcatttt atcattgtgg
     8521 tttatgttgt ccacatagtt gttgagcgtg tcttttaatc cttcggatac tgttttgagt
     8581 acattaataa actctaaggt ttcttcgtct tgaatctttg aagtgtctaa caaatcaaaa
     8641 acagtaagta ggttattaac cggcgaacgc atatcgtgtg aagccgtata ggttaaattc
     8701 tttagattgt tattgatttt ttttaaactt gccaagagta ggttacgttc ttcttcaatt
     8761 cgttttttat aagtaacatc tttggcaata gcataaacta gattttcatc cattaacgga
     8821 actgaagtcc atgccaacca aactatttcg ccacttttag taatataacg gttttcatag
     8881 tgcatcaaag tgttgccaga tattactttt tctcggcttt ttattgtttt ctctatgtca
     8941 tctttgtgaa taaaatcatt aataggcttg gccaataatt cttcctctga atatccaaaa
     9001 aggttacaaa cggctggatt tactttttta aaatacccat cataaccagc aataaaaaat
     9061 aggtcagggg aaatatcaaa aaacaattcg gttttaagtt tttgtattcg gcgtgtagta
     9121 tcgatcatct taataaggat tgtaaaagtc aaatttagta aatattctag aagtaatgaa
     9181 gtataattgg acttccttta aaaaaaggat tttgtttcat tatactaggg gttctaggta
     9241 atgttgcctt atttgggtat agatgaaagt ttgtttcgtt ttatttttta taat
//
LOCUS       DAOOBH010000091         9288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_9208_length_9288_cov_6.917253, whole genome shotgun sequence.
ACCESSION   DAOOBH010000091 DAOOBH010000000
VERSION     DAOOBH010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9208_length_9288_cov_6.917253"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..343)
                     /locus_tag="PK218_08945"
     CDS             complement(2..343)
                     /locus_tag="PK218_08945"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPW98675.1"
                     /translation="MKTKERENNNFFERVYEIARLIPEGKVTSYGAIAKALGAARSAR
                     MVGWAMNACHNLDDIPAHRVVNRKGLLTGKHHFDGTNLMQQLLENDGIKVIDNQIIDF
                     EKHFWEPKMDL"
     gene            complement(344..982)
                     /locus_tag="PK218_08950"
     CDS             complement(344..982)
                     /locus_tag="PK218_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPW98676.1"
                     /translation="MTFIIPFFSGFLAAIVGIFPPGLINMTAAKVSVTDGRRRALVFV
                     FGALIVIFIQTYISVLFAQYINKNIEIVILLREIGLAIFTLLSLYFLFFAKEPKPILN
                     KNKIQLKSKRSRFFMGMLISAINFFPIPYYVLVSITLASYKVFTFTNLSIYSLVSGVV
                     IGSFSVFYCYVVFFEKLKTKTDYFIKNMNSIIGIITGIVAVITLINVLDYYY"
     gene            complement(1052..1726)
                     /gene="trmB"
                     /locus_tag="PK218_08955"
     CDS             complement(1052..1726)
                     /gene="trmB"
                     /locus_tag="PK218_08955"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPW98677.1"
                     /translation="MGSKNKLKRFKENETFENVLQPTREEVVSGEFQYKGKWNSDFFK
                     NNNPIIVELGCGKGEYTIGLAERNPNINYIGIDVKGARFWRGAKTAIETGMNNVGFVR
                     TQIELIENIFSSHEISEIWITFPDPQIKYKRTKHRMTNSEFLKMYKRILKPEGVVNLK
                     TDSEFMHGYTLGLLHGEGHEVLYSNHNVYKNEGSPSEVTEIQTFYENQYLEINKAITY
                     IRFKIK"
     gene            1824..2879
                     /locus_tag="PK218_08960"
     CDS             1824..2879
                     /locus_tag="PK218_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW98678.1"
                     /translation="MKKNILVAPLNWGLGHATRCIPIIRELEKNNFIPIIASDGNALA
                     LLKKEFPHLLHLELPSYHIEYAKKAENFKWKLIKNSPKTIAAILSEKKIVKKWCKEFG
                     LSGIISDNRLGVYNKKVPSVIITHQLTVLSGKTTWISSKMHQFFIKNFTECWVPDFDK
                     NFNFSGKLGHLKDSDLNIHYIGILSRLKKKELPIKYDLMVLLSGPEPQRSLLEDKLFD
                     ELRKFDKKVIFIKGIVEKEQKKEQLKNITIYNFMTSAELEVAINESEKILCRSGYTTI
                     MDLIQLEKKAFFIPTPGQFEQEYLAKNFKRSGILPYSKQANFKMEQLKEIELYKGFPI
                     VLEPMDWKKLFILFESE"
     gene            complement(2853..3896)
                     /locus_tag="PK218_08965"
     CDS             complement(2853..3896)
                     /locus_tag="PK218_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW98679.1"
                     /translation="MAIKFKKTYTFALISTSYITLFSTSLLSLLLWFFEKFSISITLF
                     FALSIAVFSFFVIQYRVERFIYRRVKKIYDDVSLLESTSFRNQPITTDMATLTKQVKK
                     FATDKKLEIETLKIKEEYRREFMGNVSHELKTPLFTVQGYISTLIDGAIDDKKVRKKY
                     LERAEKGVERLVYIVEDLDMIAKLEMGDVGTQKSKFDIVELIQNVFDMLELSADHKNI
                     ILMFDRKYSKGIYVYADKEKIRQVLTNLVVNSIKYGKENGTTEVAIEDLVKNKIIVRV
                     KDNGEGIEKQNLSRIFERFYRVDKSGSRSVGGSGLGLSIVKHIIEAHDEKIYLESEFK
                     KGSEFSFTLEKDK"
     gene            complement(3896..4579)
                     /locus_tag="PK218_08970"
     CDS             complement(3896..4579)
                     /locus_tag="PK218_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW98680.1"
                     /translation="MKKKDITILLVDDEQDILEIVGYNLEQEGYKVITALDGKEAISK
                     AKKAIPQLIIMDVMMPGMDGMEACEHIRRIPELSNVIITFLTARSEDYSQVAGFEAGA
                     DDYITKPIKPKLLVSKVKALLRRLKTDEKTSDTLFVAGLEINREEYKIVKNGEELILP
                     RKEFELLYLLASKPGKVFKREEILDKVWGNEVIVGGRTIDVHIRKLREKIDEELFKTI
                     KGVGYKLEA"
     gene            4771..7512
                     /locus_tag="PK218_08975"
     CDS             4771..7512
                     /locus_tag="PK218_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPW98681.1"
                     /translation="MKLQFLLISLFISALAIAQTKGTVTGIITDKDLQNETLPFANVV
                     VKGTLVAVNTDIDGKYTFKIEPGNYTIEFSFLGYESVTKTVLVKANETTTLNLALGSG
                     SYTLQDVVIQNNSNRAKETALLLDQKNAVEMKQAIGAQELSRKGVSDVANAVIKTTGI
                     TKQEGSGDIFVRGLGDRYNSTTMNGLPIPSNNPEKKNMSLEIFSTEIVEYISIDKVYS
                     SKLFGDFAGGNIDINSKDYKGNGFFKIDIGSKINTNAVSEKDFSLQKGFNSFGFENRQ
                     IPSNALTQYKFSTLNLENKNPFAGSIGVSGGNSFNIGEQGKINFFATASFDNEYSAIN
                     EGSLKSGVNGSGVINKNFQKYTLLNYATSTTGMINLGYKINTSNKLNYNSLLINNSSQ
                     TKEEGTGYIVDIANDGNGLLRFSNYNKNTILINQLLGEHKLTAASKFNWGVAYNLVKA
                     DTPDRTQNTLRKEANGYVLSSISAPDNNRYYQYLVENELVGNGSVDFKFAKDVDDSYT
                     GKITLGYSGRIKNSDFKATQFNFKANAAFANTLVDPNNLDSFYNQENFSNDYFVITTF
                     RGNFQVPNALKPQTYVGDQIINGGYVNAEYKFHKLTTILGLRAEMIGQRIKWDTQLGG
                     TGVNLLEKNTFLPSLVMKYELNDKQNLRLGFSKTYTLPQFKERAPFIFEKFGQAYIGN
                     PYLYASNNYNLDLKWEMFPKNEELLSVTAYGKYIQNPMNEITLNSSTNDISYFNTGDY
                     GYVIGGEIEYRKLLFDNDSKKLSAGLNASYLNTTQELNADKIRNENKGQEVVFNTNTD
                     KFTGASDLLLNADISFFNEWNDKKSNLNTTLAYTFFSDRVNTIGTNGKGNLVDKSFGS
                     LDLIIKSKINQHIGIGLVTKNILNPAINRIQENNTGDVNVLSYKKGLNLSLSLNYQF"
     gene            7673..8857
                     /locus_tag="PK218_08980"
     CDS             7673..8857
                     /locus_tag="PK218_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98682.1"
                     /translation="MKNSILKVASIIAITATLLTGCSLSSDNSNDSNGFVADPNNFKG
                     TISGETITLNPTLIYKLTGKLQINDGASLTIPAGTVIEGVGGTAAYIAVAQGGKLFVA
                     GTASNPVIMTSGLATKSAGDWGGLVICGKAPINRVTGGASSAQSEVADLTYGGTNPSD
                     NSGSIRYLRIEYAGAAFNSEKEFNGVSLFGVGSETVFEYVECYHGSDDGFEFFGGTVN
                     TTGLISIGNEDDQFDWTEGWSGTNTNWYGKLDFGKGNRGIEADNFELGYLNTPIANPT
                     ITNMTLIGPGSTADGTTYLENDGLKLRRGTKGMFTNIVLSNWKTGFNVEHDECIAAVA
                     AGTLKATNVNFVADVPSKSKGKDTAGASADVSAIFTEGSATGAGNGVGTPSWAAGWSV
                     GF"
     gene            8964..9278
                     /locus_tag="PK218_08985"
     CDS             8964..9278
                     /locus_tag="PK218_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98683.1"
                     /translation="MTKKYFYITLFFCIFSLMGFAQESKIQANDVLGFYPNPVSNGKI
                     YITSKTTLEKEILIFDVLGKKVLQTTLTSKELNISTIPPGVYIIKISEGDATATRKLI
                     IR"
BASE COUNT         3284 a         1405 c         1474 g         3125 t
ORIGIN      
        1 attacaaatc catcttcggc tcccaaaaat gtttttcaaa atctataatt tgattgtcaa
       61 ttacttttat gccatcattt tcgagtaatt gttgcatcaa atttgttcca tcaaaatgat
      121 gtttcccggt caataatcct tttctgttca caactcgatg tgctggtata tcatcaagat
      181 tatgacaggc attcattgcc caaccaacca ttcttgccga acgagctgca ccaagtgctt
      241 ttgcaatcgc accataagaa gttacttttc cttcgggtat taaacgagcg atttcataaa
      301 cacgttcaaa aaagttgttg ttttctcttt ctttcgtttt catttaatag taataatcta
      361 aaacatttat taatgttata acagcaacaa tcccagtgat tatcccgata atagaattca
      421 tgtttttaat aaaataatcg gtttttgttt taagtttttc aaaaaaaaca acgtaacaat
      481 aaaaaactga aaatgaaccg attacaacac cagaaaccaa actgtaaata gacaaatttg
      541 tgaaagtgaa aactttataa gaagccaaag ttatactcac taaaacataa taaggaatag
      601 ggaaaaaatt aatcgccgat atcaacatac ccataaagaa acgacttctt ttacttttta
      661 gttggatttt atttttgttg agtattggtt ttggctcttt tgcaaaaaat aaaaagtaaa
      721 gcgataataa cgtaaatatt gccaatccaa tttcacgaag taaaatgaca atttcaatat
      781 ttttattgat gtactgtgca aaaagtacag aaatataggt ttgaataaaa ataactatca
      841 aagcaccaaa aacaaaaact aaagcacgtc ttcttccgtc ggttacactc acttttgcag
      901 ctgtcatatt gattaatcca ggtggaaaaa tacctacaat agctgctaga aatcccgaaa
      961 aaaaaggaat aataaatgtc ataaattcca attacttaaa tcccaaattc caatccagaa
     1021 attttgggtt ggacaatgac atttaaaaat attatttgat tttaaaacga atatacgtaa
     1081 ttgctttgtt aatttccaaa tattgatttt cgtaaaatgt ttgaatttca gttacttcac
     1141 ttggcgaacc ttcattttta tacacattat gattggaata caaaacttca tgaccttcgc
     1201 catgtaataa tcctaaggta taaccatgca taaattcgct gtcggttttc aggtttacaa
     1261 caccttcggg ctttaaaatt cttttgtaca ttttcaaaaa ctcagaattg gtcattctgt
     1321 gttttgttcg tttgtatttg atttgaggat cgggaaaagt tatccaaatt tcagaaattt
     1381 catgagatga aaaaatattt tcaatcaatt ctatttgagt tctaacaaaa ccaacattgt
     1441 tcattccagt ttcaatcgct gtttttgcgc cacgccaaaa tcgggcgcct ttaacatcaa
     1501 ttccgatata gtttatattt ggatttcttt cggctaatcc tattgtatat tctccttttc
     1561 cacatcctag ctcaacaata ataggattat tgtttttaaa aaaatcggaa ttccattttc
     1621 ctttatattg aaattctccg cttacaactt cttctctcgt tggttgaagt acattttcaa
     1681 aagtttcgtt ttctttgaaa cgctttaatt tattcttgct tcccacaaat ttaatttttt
     1741 ggtaaaatta aggaaatata aattacataa aatctttttt actttgttaa aattaataag
     1801 cgtaacttca ctacttgaaa actattaaaa aaaacatact cgttgcgcca ctaaattggg
     1861 gtttaggtca tgccacacga tgcattccga taattagaga attggagaaa aacaatttta
     1921 ttccaatcat tgcatcagat ggaaatgctt tggcattgct aaaaaaagaa tttccgcatt
     1981 tattgcattt agaattgcct tcgtatcata ttgaatatgc caaaaaagca gaaaatttta
     2041 aatggaaatt aattaaaaat agtccaaaaa ctatagctgc tattctatct gaaaaaaaga
     2101 ttgtgaaaaa atggtgtaaa gaatttggtc ttagcggaat aatttctgat aatcgtttag
     2161 gtgtttacaa caaaaaagtt ccaagtgtaa ttatcacgca tcaacttact gttttatctg
     2221 gaaaaaccac ttggatatcg agtaaaatgc atcaattttt tattaaaaac ttcacagaat
     2281 gttgggttcc tgattttgat aaaaatttta atttttcagg aaaactaggt catttaaaag
     2341 atagcgattt aaatattcat tatattggaa ttttaagccg tttgaaaaaa aaagaactac
     2401 ctattaagta tgatttgatg gttttgctat ctggtccaga accgcagcgg agtttgctgg
     2461 aagacaaact ttttgatgag ttaagaaaat ttgataaaaa agtaattttc atcaaaggaa
     2521 ttgttgaaaa agaacaaaaa aaagaacagc taaaaaacat tacgatttat aatttcatga
     2581 cttctgctga attggaagtt gcaattaatg aaagtgaaaa aatactttgt cgttcgggtt
     2641 acacaactat tatggattta attcagttag aaaaaaaagc gtttttcatt ccaacacctg
     2701 ggcaatttga gcaggaatat ttggctaaaa attttaaaag aagtggaatt ttaccgtatt
     2761 caaaacaagc taattttaaa atggaacagt taaaagaaat tgaactttac aagggttttc
     2821 caattgtttt agagccaatg gattggaaaa aattatttat ccttttcgag agtgaatgaa
     2881 aattcagagc cttttttaaa ttcgctttct aaataaattt tttcgtcgtg tgcttcaata
     2941 atatgtttta ctattgacaa tcctaaccca gaaccaccaa cgcttctgga gccgcttttg
     3001 tcaacacgat aaaaacgttc aaaaattcga gataaatttt gtttttcaat tccttcgcca
     3061 ttatctttta ctctaacaat gattttgttt ttcactaagt cttcaatggc aacttctgtt
     3121 gtgccgtttt ctttgccata ttttatggaa tttacaacaa gatttgttag tacttgccgt
     3181 attttttctt tatcggcata gacataaatt cctttactat actttcgatc aaacatcaaa
     3241 attatgttct tatgatcggc actcaattca agcatatcaa aaacattttg aatgagttcg
     3301 acaatatcaa atttagattt ttgcgttcca acatcgccca tttctagttt ggcaatcatg
     3361 tctaaatctt ccacaatgta caccaatcgt tcaacacctt tttcggcgcg ttcgagatat
     3421 ttttttctta cttttttatc gtctattgca ccgtcaatca acgttgaaat ataaccttgt
     3481 acggtaaata atggtgtttt taattcatga gaaacatttc ccataaactc tcttctgtat
     3541 tcttctttta tttttaaagt ttctatttcg agttttttat ctgtggcaaa cttttttact
     3601 tgttttgtaa gtgttgccat gtcagtagtg attggttgat ttctgaacga agtagattct
     3661 aataaagaaa cgtcatcata tattttttta actcttctgt aaataaatcg ttctacacga
     3721 tattgaatca caaaaaaaga aaacacagca atagataacg caaaaaataa agtgatgcta
     3781 atggagaatt tttcaaaaaa ccacaataac agcgataata aacttgttga aaacaacgta
     3841 atataagaag ttgaaattaa agcgaaagtg tatgtctttt taaattttat agccattatg
     3901 cttctagttt gtagccaacg ccttttatag ttttaaataa ttcttcatct attttttctc
     3961 ttaattttcg gatatgaaca tcgatagttc taccgccaac aatgacttca tttccccaaa
     4021 ctttatctag aatttcttcc cgtttgaata cttttccagg ttttgaagct aataaataaa
     4081 gaagttcaaa ttcttttcta ggcaaaatca attcttcacc atttttgaca attttatatt
     4141 cttcacggtt aatttctaat cctgcaacaa aaagagtatc cgatgttttt tcatcagttt
     4201 ttaaacgacg aagtagcgct tttactttgc ttactaataa ttttggttta attggttttg
     4261 taatatagtc gtctgcacca gcctcaaaac ctgctacttg agaataatct tcgcttctag
     4321 ctgttagaaa agtaataata acattgctca attcaggaat tcgtctgatg tgttcacaag
     4381 cctccatgcc atccattcca ggcatcatta catccattat aatcaattgt ggaattgctt
     4441 tttttgcttt gctgatggct tcttttccgt ctaaagcggt aatcactttg taaccttctt
     4501 gttcaagatt atagccaaca atttctagta tatcttgctc gtcatctact aataagatag
     4561 taatgtcctt ttttttcata gtaataacct ttaatggttt caatgcaaaa gtaaagataa
     4621 tataaaatgt aattaaaata tgtcgatgag ttaaccatta tttaaactaa taacaatttg
     4681 gtaatcttta gagaacacaa atctaatatg caactaactt cttctttaca ttgaggcagt
     4741 tcctttgcac taaatttatt aatagaaaaa atgaaattac aatttttact tatttcctta
     4801 tttattagcg cattagcaat tgcacaaact aaaggaactg taactggaat tatcacagat
     4861 aaagatttac aaaacgaaac attgcctttt gcaaatgtgg tggttaaagg aactttagtt
     4921 gctgtaaata cagatattga cggaaaatat actttcaaaa ttgaaccagg aaattatact
     4981 attgaattta gttttttagg ttatgaaagt gttacaaaaa cagttttagt taaagctaat
     5041 gaaaccacaa ccttaaactt agcacttggt tctggaagtt atacattaca agatgttgtt
     5101 attcagaata attcaaacag agcaaaagaa acagcattgt tgttggatca aaaaaacgct
     5161 gttgaaatga aacaagctat tggagcgcaa gagctttcta gaaaaggtgt aagcgatgtt
     5221 gctaatgcgg ttataaaaac aactggtatc actaaacaag aaggttcggg tgatattttt
     5281 gttcgaggac ttggagatag atataattca accaccatga acggtttgcc aattccttca
     5341 aataatccag aaaagaaaaa tatgagtttg gaaatatttt caacagaaat tgtagaatac
     5401 atttctatcg ataaagtata tagcagtaag ttgtttggtg attttgcagg tggaaatatt
     5461 gacataaact ctaaagacta taaagggaat ggttttttta aaattgatat tggttcaaaa
     5521 ataaacacta atgccgtttc tgaaaaagat ttttcgcttc aaaaaggatt caatagtttt
     5581 ggttttgaaa acagacaaat tccttcaaat gcgttgacac aatacaagtt tagcacttta
     5641 aatctagaaa acaaaaatcc gtttgctggt tcaataggtg tttcaggcgg gaattctttt
     5701 aatattggtg agcaaggaaa gattaatttc tttgcaacag catcgtttga taatgagtat
     5761 agtgctatta atgaaggttc tttaaaaagt ggtgtaaatg gaagtggtgt aatcaataaa
     5821 aactttcaaa aatatacttt attaaattac gctaccagta caacaggaat gattaattta
     5881 ggatataaaa tcaatacttc caataaacta aattacaact cattattaat caacaattct
     5941 tcacaaacta aagaagaagg aactggttat attgtagata ttgccaatga tggaaatgga
     6001 ttgttgcgtt tttctaacta taacaaaaac acaattttaa taaaccaact tttgggtgag
     6061 cacaaattga cagccgcttc caagtttaat tggggtgttg cttataattt agtaaaagcc
     6121 gatacgccag atagaactca aaacacttta agaaaagagg ctaatggcta tgttttgtcg
     6181 agtatttcgg ctcctgataa caatcgttac taccaatatt tagttgaaaa cgaattagtg
     6241 ggtaatggtt ctgtcgattt taaatttgct aaagatgttg atgatagcta tactggtaaa
     6301 atcactttag gttacagcgg aagaattaaa aacagcgatt ttaaagcaac gcaatttaac
     6361 ttcaaagcaa atgcggcatt tgctaatact ctagttgatc caaataattt agattccttt
     6421 tataatcaag aaaatttcag taatgattat tttgtgatta ccacatttag aggaaacttt
     6481 caagttccaa atgcattaaa acctcaaacc tatgtaggtg accaaattat aaatggtggc
     6541 tatgttaatg ccgaatataa atttcataaa ctaactacca tactaggttt aagagcagaa
     6601 atgataggac aacgtataaa atgggatacg caattgggtg gaacaggtgt aaatcttttg
     6661 gaaaaaaaca cttttttacc aagtttggta atgaaatatg aattgaatga caaacagaat
     6721 ttaagattag gatttagtaa aacctatact ttacctcagt ttaaagaaag agcaccattt
     6781 atttttgaaa aatttggaca agcctatatt ggaaatccct acttgtatgc ttcaaataat
     6841 tataatttag atttaaaatg ggaaatgttt ccaaaaaacg aagagctact ttctgtaact
     6901 gcttatggaa aatacatcca aaacccaatg aatgaaatta cacttaactc ttcaactaat
     6961 gatatttctt attttaatac tggagattat ggatatgtta ttggaggaga aattgaatat
     7021 agaaagttgc tttttgataa tgattctaaa aaattatccg ccggattaaa tgcttcctac
     7081 ttgaatacca ctcaagaatt aaatgccgac aaaattagaa acgaaaataa aggtcaagaa
     7141 gttgttttta atacgaatac tgacaaattt acaggagcat cagatttgtt gttaaatgca
     7201 gatatttcgt tttttaatga atggaatgac aaaaaaagta acctaaacac aacattagct
     7261 tatacattct tttcggatag ggtaaatacc attggaacaa acggcaaagg aaatttagtt
     7321 gacaaatcat tcggctcact tgatttaatt ataaaatcta aaattaacca gcacatagga
     7381 attgggcttg taactaaaaa cattttaaac ccagcaataa atagaattca ggaaaacaat
     7441 actggagatg tgaatgttct gtcctataaa aaaggtctta atttgagttt gagtttaaat
     7501 tatcaatttt aacaatactt cattatgaaa gtggtatgtt aatttaaggt aaaaaagaag
     7561 cgttaagtta aacttaaatt gattttttat taaaacaact ataatcccaa gttaacatcc
     7621 ttttggattt caatttcaat atttgtaaaa caaaataaca aattatttat ttatgaaaaa
     7681 ttcaattctt aaagttgcaa gtatcattgc aataaccgca accttattga ctggctgttc
     7741 attaagttcg gacaattcaa atgattcaaa tggatttgtg gctgatccga ataattttaa
     7801 aggaacaatt tcgggagaaa ccattacatt aaatccaacc ttaatttaca aattaacggg
     7861 taaattacaa attaacgacg gtgcttcttt aacgatacca gcaggaactg taattgaagg
     7921 agttggcgga accgctgcgt atattgccgt tgctcaaggc ggaaaattat ttgttgcagg
     7981 aacagcttca aatcctgtta ttatgacaag tggattagct actaaaagtg ccggagattg
     8041 gggcggatta gttatttgtg gaaaagcacc tattaatcgt gttactggtg gagcaagttc
     8101 tgctcaatcg gaagttgccg atttaactta tggcggcaca aatccaagcg ataattctgg
     8161 gtctatcagg tatttaagaa tagaatatgc tggagcagct tttaacagcg aaaaagaatt
     8221 taacggtgtt tcattatttg gagtgggttc ggaaacagtg tttgagtatg tagaatgcta
     8281 tcatggctca gatgatggct tcgaattctt tggaggaacc gtaaatacta ctggacttat
     8341 ttcaattgga aatgaagacg accaatttga ctggacagaa ggttggagcg gaacaaacac
     8401 aaattggtat ggaaaattag atttcggaaa aggcaataga ggaatagaag ctgataattt
     8461 tgaattggga tatttgaata caccaattgc aaacccaaca atcactaata tgactttaat
     8521 tgggccagga agtactgcgg atggaacaac ttatttagaa aacgatggat taaaattaag
     8581 aagaggtact aaagggatgt ttacaaacat agtgttaagt aattggaaaa caggatttaa
     8641 tgtagagcat gatgaatgta ttgctgcggt tgcagcagga actttaaaag caactaatgt
     8701 taatttcgtt gctgatgttc cttcaaaatc aaaaggaaaa gatactgctg gtgcttctgc
     8761 tgatgtaagt gcaatattca cagaaggttc tgctactgga gctggaaatg gagttggaac
     8821 tccatcttgg gcagctggtt ggtcagttgg attctaataa aaaaagcaaa caaacaaaaa
     8881 tcctgaataa attattattc aggatttttt ttggcattat atttgaaata atttttatac
     8941 atttgtataa tctaaaaagt gcgatgacga aaaaatactt ttatatcact ttgttctttt
     9001 gtatattttc tttaatgggc tttgctcaag aaagtaaaat acaagctaat gatgttttag
     9061 gtttttatcc taatcccgtt agtaatggta aaatctatat cacctcaaaa accacattag
     9121 aaaaagaaat tcttattttt gatgtattgg gcaaaaaagt attgcaaact accttaactt
     9181 caaaagaatt gaacattagt accattccac ctggagttta catcataaaa ataagcgaag
     9241 gtgatgctac tgctactaga aaattaataa tcaggtagtt gtttttat
//
LOCUS       DAOOBH010000092         8905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_9665_length_8905_cov_4.898983, whole genome shotgun sequence.
ACCESSION   DAOOBH010000092 DAOOBH010000000
VERSION     DAOOBH010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9665_length_8905_cov_4.898983"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PK218_08990"
     CDS             complement(<1..190)
                     /locus_tag="PK218_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW98684.1"
                     /translation="MKFTFKIILILAILFSFVQCKKEVKTIENAIVSDEIQYAKGFSL
                     ENYEGYSVVTVKNPWPKAT"
     regulatory      241..438
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            521..2473
                     /locus_tag="PK218_08995"
     CDS             521..2473
                     /locus_tag="PK218_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPW98685.1"
                     /translation="MKKTIRISAFFALITSCALAQEKDSLAVNALKEVVVSDTKFLQN
                     REKSGKVIEVISAADLEKKSGQSLATVLSQVTGLEINGNQSANGKNLGYYIRGGKNRQ
                     VLILIDGIPVTDASGISFEYDLRLLPVEQVERIEIMKGAASTLYGTGAATGVINITLK
                     KSSKKEIEGNAYMNIGSNNTADNHHYNGQEFNQGISVNGTVSKVNYFAGLNSTETNGM
                     SQIAAPTSDTDYETDRFSRQNVLAKLGYKATEKLTLDFFGNYDRIKNDYDLSFDNTGF
                     NDTDINKTKSEQIRFGFAPKYQYNKGEFVLNSGFTKITRDYDELNTFSNTVDYSLYES
                     RSIVVDGYNKYNFSNQLAIILGATYQFNDMKSQTPFSTIEKELTKFNIVDGYTTFVYN
                     SNFGLNVNTGGRLNHHSAYGNNFVFNFNPSYNFKTSFPFKMLASYSTAYVTPSLYQLY
                     SEYGNDTLTPEKNATIEAGFETELMNKKLKLNTVAFYRDQNDAINFYFNPVTFDSFYV
                     NVDGSTKAKGVESSLSYAVLPKLHINANYTFTQVDEVLNRLIPKHKANASLEYQPSSR
                     TLFNVSYQYFDARNDAFYDANTFSVLPIKLGSYQLVNLLVKHELIKNRLTVFGAATNI
                     LNEEFVENVGYATRGRNFKIGLNIVL"
     gene            complement(2565..3119)
                     /locus_tag="PK218_09000"
     CDS             complement(2565..3119)
                     /locus_tag="PK218_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPW98686.1"
                     /translation="MRKIIAALNMTIDGFCDHTAGIPDENIHQHYTELLGQGDAILYG
                     RTTYQLMEFWQTVLDNPSEEKSMNDFAMAIDKIPKIVFSRTIKNVEWKSATIAKKNLK
                     EVVLELKQLPGKAIFVGSRSLIIQLMKLGLIDEFQLCIHPMVEGKGLPLFENINDRII
                     FKLVKNKIFTNGAIILYYQPKKNS"
     gene            3435..3971
                     /locus_tag="PK218_09005"
     CDS             3435..3971
                     /locus_tag="PK218_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016361994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW98687.1"
                     /translation="MINEIQIVGCRKKQRDAQRQVYDYMSPKLFRTCKRYLKKQEEIE
                     EVLADAFYTIFTKIDQLKENNAFEAWARKITVNQCLLHLKRNVNFNLYLEDTSIYLHP
                     ETASATQLEEEDLLKLLEVIPDGCKTIFNLFVIEGYGHKEIAEMLNISEGTSKSQLNV
                     AKTKLKELINKFYYQNAK"
     gene            3995..4930
                     /locus_tag="PK218_09010"
     CDS             3995..4930
                     /locus_tag="PK218_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98688.1"
                     /translation="MENKDDIFEQFKTAANSAEQKEFAAKDKVWNRVENKLDNKLLTK
                     ENTFWRKLAVAASVLLVASIGYQYLKEDKINITPTNDVVVIDTLKTITDSTTNNDIVT
                     VEKKQNPAINANAEMILKEQINTQSQVVVRDTLRRMMPKMIVSKSESIAESDDLMELQ
                     KPAATVANSYDSYNGIAKRKDNVRGVKQHEGYLNYSDDAKEAKQTSKKEAPLLVIDDK
                     VSDTQTLGKLDPKDLENIILLPNPLYIINGIEYTEKELFGPNPTSPYAPLSQQNIETL
                     SILQNEKATEVYGEKGKKGVVIIATKGGIPAAKKK"
     gene            4964..>5207
                     /locus_tag="PK218_09015"
     CDS             4964..>5207
                     /locus_tag="PK218_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPW98689.1"
                     /translation="MKKFKPFSTVLAMLICFVSLAQQKTITGVVSDALGPLPGTTVVV
                     KNTTRNTQTDFDGKYNIKASKGEILVFSYVGYTSKAV"
     assembly_gap    5208..5307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5509..7059
                     /locus_tag="PK218_09020"
     CDS             5509..7059
                     /locus_tag="PK218_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPW98690.1"
                     /translation="MVSQQVQGYAKGLSISQSNTNAIVIRGSRSVTGTTDIIPATNDY
                     EDYEAFAENVFESPKASPLSTFSIDVDNASYTNIRRFINNGQKVPKDAVRIEEMMNFF
                     KYKYPQPTNEHPFSINSEYSDCPWNTNNKLVRIGLQGKNIDDSKLPASNLVFLIDVSG
                     SMFAANKLPLLKESLKVLVNQLRKDDRVAIVVYAGAAGLVLPPTSGDKKMTIIDALEK
                     LQSGGSTAGGAGIQLAYQTAKEHFIKGGNNRVVLATDGDFNVGASSDNDMQRLIEEER
                     KSGVFLTCLGFGMGNYKDSKMETLANKGNGNYAYIDNIQEANRFLGKEFKGSMFAIAK
                     DVKIQIEFNPTFVQAYRLIGYENRKLRPEDFKNDEIDAGELGSGHTVTALYEIVPVNV
                     KSDYFKPIEDTKYSKVENKGVNYQNELATIKFRYKKPNGDKSIEMVSTIASASKSLNT
                     ASDDYKFTAAVAWFGLNLRDSKLVSNKSLVAIKKLAKEGLANDDEGYKAEFIRLIDGG
                     NTFIASKN"
     gene            7275..7538
                     /locus_tag="PK218_09025"
     CDS             7275..7538
                     /locus_tag="PK218_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98691.1"
                     /translation="MKNVSLILASVVMMLIASCKGETEKTTEKTVVIEKQVEATPVKE
                     VEEETDGTSVSISKDGVDFSTKDGDKKTKVEVKDGAASVSSKK"
     gene            8356..>8905
                     /locus_tag="PK218_09030"
     CDS             8356..>8905
                     /locus_tag="PK218_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW98692.1"
                     /translation="MNENHSDDQYGRARVQDSPELIAYYKELEKLGAGALWTVANDIE
                     PWEPRSSSVPMLWKYDNLRELVVKSSDLVTPEQAGRRVVYLVNDKRKDVSAAVGWLYT
                     GIQVTRPGESTSAHRHKASALRFIMEGEGGYTVVNGNKITFEVNDFVITPNSTWHEHG
                     VAEDGKTCIWQDGLDIPLVNALE"
BASE COUNT         3164 a         1513 c         1528 g         2600 t
ORIGIN      
        1 tagtcgcttt tggccaaggg tttttaacag taactaccga gtaaccttcg tagttttcga
       61 gagaaaagcc tttggcatac tgaatttcat cggacacaat tgcattttct atcgtcttta
      121 cttctttttt gcattgaaca aaagaaaaaa gaatggctag tattaaaatg attttgaatg
      181 tgaatttcat aaaatgaagt tttcacaaaa gtaaactttt gtttttaata atttaagtta
      241 catttgcacc tgaattttgg ttgttattca cttttgtgaa aaacattaaa agggaatttg
      301 gttcaaatcc aaagctgttc ccgcaactgt aagctatcaa gcttgttgtc atctacatta
      361 ccattgttcg ccacggtgaa tgagaaggtc gacaacaaga cgcaagccag gaaacctgcc
      421 tatattcatt gagtaacaaa ctttcgggaa aaaaggtttg ggtatgggta aattctatgc
      481 ttttctccca acattagtaa actattgttt aatttaaaaa atgaaaaaaa caattcgcat
      541 cagtgcattc tttgcattga taacctcttg tgcattggcg caagaaaaag attcgctagc
      601 ggtaaacgca ctaaaagaag tagtcgtatc agacactaaa tttttgcaaa acagagaaaa
      661 atcagggaaa gttatcgaag tgatttcggc tgccgattta gaaaaaaagt caggtcaaag
      721 tttggccacg gttttaagtc aggtaacagg tcttgaaatc aatggaaacc aatcggctaa
      781 tggaaaaaac ttgggctatt acattcgtgg tggaaaaaat cgccaagtct taattcttat
      841 tgatggcatt ccagttaccg acgcttcggg cattagcttt gagtacgatt tgcgtttgct
      901 tcccgtagaa caagtagaac gaatcgagat tatgaaaggt gctgcgagta cgctctacgg
      961 tacaggtgca gccacaggag taatcaacat tacgttgaaa aaatcttcca aaaaagaaat
     1021 cgaaggaaat gcctatatga acatcggttc aaacaatact gcggataatc atcattataa
     1081 cggtcaagaa tttaaccaag gtatttcggt taatggaacc gtttcgaaag taaattattt
     1141 cgctggtttg aattctaccg aaaccaacgg catgtcacaa attgctgctc caacttcgga
     1201 taccgactat gaaaccgacc gtttctctag acaaaatgtg ttagcaaaat taggatataa
     1261 agccaccgaa aaactaacgt tagatttctt tgggaattat gacagaatca aaaacgatta
     1321 cgatttatct tttgacaaca ctggttttaa tgatactgat ataaacaaaa ccaaatcaga
     1381 acaaatccgt tttgggtttg cacctaagta ccaatacaac aaaggcgaat tcgttttaaa
     1441 ctcaggtttt acaaaaataa cgcgcgatta tgatgagcta aacactttca gtaacaccgt
     1501 tgattattcg ctttatgaat cgagaagtat agtggtagat ggttataaca aatataattt
     1561 ttcaaaccaa ttggcaatta ttttaggtgc tacatatcag tttaatgaca tgaaaagcca
     1621 aacacctttt agcactatcg aaaaagagtt aaccaagttc aatattgtag atggttatac
     1681 tacatttgtc tataactcta actttggtct aaatgttaac actggcggac gattgaatca
     1741 tcacagtgct tatggcaata attttgtgtt taatttcaat ccatcttata attttaaaac
     1801 tagttttcca tttaaaatgt tggcatcata tagtactgcc tatgttacgc caagtttata
     1861 ccaactctat tctgaatatg gaaatgacac attaacaccc gaaaaaaatg ccaccatcga
     1921 agcaggtttt gaaaccgaat tgatgaacaa aaaactaaaa ttaaacaccg ttgcatttta
     1981 tagagatcaa aacgatgcca tcaatttcta tttcaatcct gtcacctttg actcttttta
     2041 tgtaaatgtg gatggaagta ccaaagccaa aggtgtcgaa agctctttga gttatgctgt
     2101 tttaccaaaa ttacatatta acgccaacta tactttcact caagtagatg aagtactaaa
     2161 tcgattgata ccaaaacaca aagccaacgc tagtttagag taccaaccat caagcagaac
     2221 actttttaac gttagttacc aatactttga tgccagaaac gatgcgtttt atgacgccaa
     2281 tacttttagc gttttaccaa tcaaactagg ttcttaccaa ttggtaaacc ttttagtaaa
     2341 acacgaacta attaaaaatc gtctaaccgt ttttggtgca gcaactaata ttttaaacga
     2401 agaatttgta gaaaatgttg gctatgcaac acgcggaaga aacttcaaaa tcgggttaaa
     2461 cattgttttg tagtttaatc aaaaaggcta gctgtgaggt tagccttttt ttatctattt
     2521 ctaattcaaa aaaaatattg ttattccttt gttactttaa gcattcatga attctttttt
     2581 ggttggtaat acagaattat ggcaccattc gtaaaaattt tattcttaac gagcttaaaa
     2641 ataatcctgt cgttaatgtt ttcgaataat ggcaacccct tgccttcaac cattggatga
     2701 atgcaaagtt gaaattcatc aatcaaaccc agtttcatca gctgtataat taaactgcga
     2761 ctaccaacaa aaatagcttt tcctggaagt tgcttgagtt ctaaaaccac ttcttttaag
     2821 ttcttttttg caattgtggc acttttccat tctacatttt ttatggtacg agagaaaact
     2881 atttttggaa ttttatcaat tgccatagca aagtcattca tcgatttttc ttcggaaggg
     2941 ttatccagca cagtttgcca aaattccatt aattgatagg tagttctgcc atatagaatt
     3001 gcatctcctt gacctaataa ttcagtataa tgctgatgaa tattttcatc aggtattcca
     3061 gcggtatggt cacaaaaacc atcaattgtc atatttaatg ctgcaattat ttttctcata
     3121 ctatttccct ttttgattta tgaatattgt ttcttcaaaa aacatttgac gcaattttga
     3181 atctctaaat taatgtaact aattggtctt tagttttggt taaaaataat aatttccaat
     3241 tttaaaaaaa agttattact attaatatct tgacaaatca ctttttttta ttctctataa
     3301 aaaagcggtt atttcattag taaacataaa gaaacaacaa aaacaataaa tcccacttaa
     3361 aaataatttt aaaaaaaatc attcccgtcc caaccttttc cattaaattg tgctctatat
     3421 aaaaaacacc ctttgtgatt aacgaaatac aaatagttgg ctgccgaaaa aaacaacgcg
     3481 atgcgcaaag gcaagtttat gactatatgt cgccaaaatt gtttcgcacc tgcaaacggt
     3541 atttaaaaaa acaagaagaa atagaagaag tattggctga tgcgttttat accatcttca
     3601 ccaagataga ccaattaaaa gaaaacaacg ccttcgaagc atgggcacga aaaataaccg
     3661 tcaatcaatg tttgttacat ctaaaacgaa acgtcaattt taacctctat ctcgaagaca
     3721 catctatata cctgcatccc gaaacggcat cagcaaccca actggaagaa gaagatttgc
     3781 taaaactact ggaagtcatc cccgatggat gcaaaaccat cttcaacctc tttgtcatcg
     3841 aaggatatgg tcacaaagag atagccgaga tgctcaatat atcggaaggc acatccaaat
     3901 cgcaattgaa tgtagccaaa acaaaactaa aggaactcat caacaagttc tattatcaaa
     3961 acgctaagtg atactgaaat aaattcagcc aaaaatggaa aacaaagacg acatattcga
     4021 acaatttaaa accgccgcca acagcgcaga gcaaaaagaa tttgctgcca aggacaaggt
     4081 ttggaatcgt gtagaaaaca aactcgacaa caaattgtta accaaagaaa ataccttttg
     4141 gagaaaacta gcagtggcag catcggtatt gttggtcgct tctattggtt atcaatatct
     4201 aaaagaagac aaaatcaaca tcactcctac caatgacgta gttgttattg acaccctaaa
     4261 aaccattacc gatagcacca caaacaatga catagtaact gttgaaaaaa aacaaaatcc
     4321 agccataaac gctaacgccg aaatgattct gaaagaacaa atcaacaccc aaagccaagt
     4381 ggttgttcgt gatacgctaa gaagaatgat gcctaaaatg attgtttcaa aaagcgaatc
     4441 catagcagaa agtgatgacc ttatggagct tcaaaaacca gccgcaacag ttgccaatag
     4501 ttatgatagc tataatggaa ttgctaaacg aaaagataat gtacgtggcg taaaacaaca
     4561 tgaaggttat ttaaactata gtgatgatgc caaagaagca aaacaaactt ctaaaaaaga
     4621 agcacctctt ttagttattg acgataaagt atcagacaca caaaccttag gaaaactaga
     4681 cccaaaagat ttagaaaaca tcattcttct tcccaatcca ttatatataa tcaatggtat
     4741 agaatatact gaaaaagaac tttttggacc aaacccaacc agtccttatg cacctttaag
     4801 ccaacaaaac attgaaaccc tttcgatact gcagaacgaa aaggcaaccg aagtttatgg
     4861 cgaaaaagga aagaaaggtg tcgtcatcat tgccaccaaa gggggaatac ccgctgcaaa
     4921 aaagaagtag caaaagatta ctaacatttt aaaaatcaac atcatgaaaa aatttaaacc
     4981 attttcaacg gttcttgcta tgcttatatg tttcgtaagc ttggcacaac aaaaaaccat
     5041 cacaggcgta gtttcggatg ctttaggacc attaccaggt accactgtag tagtaaaaaa
     5101 caccacacga aacactcaaa ccgactttga tggaaagtac aacatcaaag cctcaaaggg
     5161 cgaaatacta gtctttagtt atgttggcta tacctcaaaa gccgttannn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnntca aagggcgaaa tactagtctt tagttatgtt
     5341 ggctatacct caaaagccgt taaggttggc aacagcacaa ctattaacgt gaaacttgaa
     5401 agcgcacaaa cacttcaaga agttgtcgta gtttcctatg atgataaaaa agaatctgaa
     5461 tatgatagtt atattggtaa agctgttatg gatactaaag cacgatcaat ggtgagtcag
     5521 caagtgcaag ggtatgctaa agggttgtct atttcacaat ccaatacaaa tgcaatagta
     5581 attagaggaa gcagaagcgt tactggaaca accgacatta ttcctgctac taacgattac
     5641 gaagactatg aagcgtttgc agaaaacgtt tttgaaagtc caaaagcatc gccgctttct
     5701 accttttcta ttgatgtcga taacgcatcc tataccaaca ttcgccgttt tataaacaac
     5761 ggacaaaaag tgcctaaaga tgccgtgcgt attgaggaaa tgatgaactt ctttaaatac
     5821 aagtatccac aaccaacaaa cgagcatcca ttttcaatca attccgaata cagcgattgc
     5881 ccgtggaata caaataacaa attagtgcgc attggcttgc aaggcaaaaa cattgacgac
     5941 tcaaaactac ctgcgtctaa ccttgttttc ttaattgatg tttcgggttc gatgtttgct
     6001 gctaataaat tgccgttgct aaaggaatcg ttgaaagtat tggtcaatca gttgcgcaaa
     6061 gacgaccgag ttgccattgt ggtctatgct ggtgcagctg gattggtctt gcctcctact
     6121 tcgggcgata aaaaaatgac gattattgat gcgttagaga aattgcaatc tggtggaagc
     6181 accgctgggg gtgccggaat tcaattggca taccaaactg caaaagaaca tttcatcaaa
     6241 ggtggcaaca accgcgtagt attggcaacc gatggcgatt ttaacgtagg cgcatcgtct
     6301 gacaatgata tgcaacgctt gatagaagaa gaacgcaaat cgggcgtatt tttaacctgt
     6361 cttggattcg gaatgggtaa ttataaagac agcaaaatgg aaacgttagc taacaaaggt
     6421 aacggaaact atgcctacat tgataatatt caagaagcca atcgtttctt aggaaaagag
     6481 tttaaaggtt cgatgtttgc cattgccaaa gatgtgaaga ttcagattga attcaaccca
     6541 acctttgtac aagcctaccg attgattggt tatgaaaata gaaaactacg tccagaagac
     6601 ttcaagaatg atgaaataga tgctggcgaa ttgggtagcg gacacacagt aacggcattg
     6661 tatgaaattg ttcctgtaaa tgtaaaaagt gactatttta aaccaattga agatacgaaa
     6721 tattcaaaag tggaaaacaa aggcgtaaac tatcaaaatg aattggcaac gattaaattc
     6781 cgatacaaaa aaccaaatgg cgataagagc attgagatgg tgagtacaat tgcttcggct
     6841 tcaaaatcgt tgaatactgc atcggatgat tataaattta cggctgcagt agcttggttt
     6901 gggttgaatt taagagattc gaaactggta agcaataaat cattagtagc tattaagaaa
     6961 cttgccaaag aaggattggc caacgatgac gaaggttata aagcagaatt tattagattg
     7021 attgatggtg gaaatacttt tattgcttct aagaattaat tgtattcata gttgggtgtt
     7081 ttaatgttga aaacctaata agtgtcaaag cttgttaggt ttttttttgc ttggtggttt
     7141 gtagctactg ccgttggatt tcttttaaaa ttaaaaactc atcatggcaa ttgtataaac
     7201 aattagaaaa accatgtaat ttaaagtctt ggtgtttagt ataattttac aaaagaaatt
     7261 aaaaccaata tagtatgaaa aatgtaagcc ttattttagc cagtgtagtc atgatgttaa
     7321 ttgcatcttg taaaggagaa actgagaaaa cgactgaaaa aacggttgtt attgaaaagc
     7381 aagtagaagc aactcctgta aaagaagttg aggaagaaac cgatggtact tcagtaagta
     7441 ttagtaaaga tggtgttgat ttttctacca aagatggaga taaaaaaaca aaagtagaag
     7501 taaaagatgg tgctgctagt gtttcttcta aaaaataaaa aacaaaaaat ctaaatgttt
     7561 gtttttgttt ctgtaacgaa cttcaatgtt acaggaacta ccaaatcttt gttgaagtgc
     7621 tctcacttta acaaagattt tttttatttt tatctgcaaa aaattcaaaa tttccgatag
     7681 ttttttttag tttctcaact caattatcag aactctaatt atcctcagtt ttacaaattc
     7741 aaaagtttta gtatgctttt ttctattcat gaactattag cttataatta gaaaaaaaag
     7801 ttgtattctt attcactttt tttttaaaaa ataggcttct gacttgtata gcttttttat
     7861 tgtttttctt tagtgttttt taggtagatt tttacagttt agaaaaaaac taattgaatg
     7921 ctctgtgaca aataaaatca aacatacatt aagaagtcaa aataacaaca aacacctaca
     7981 aatcagatat ttttaacttt atataaatag ctgaattata tagtaattca caattcaaag
     8041 aacttaaaac tcttaaatat ttttttacta tatttgacat agatttagtt tagctttaag
     8101 cacttaacgt attgattttc attattttga aaaaatacaa gttgtttttt cactcttatg
     8161 atttcagtag ctttcatcaa aatattatcc caacgggtta attttaaaga aattataaat
     8221 ttaacttttg aattactcaa aataataaac cccgacaata ctcaaaacta ttttagggtt
     8281 ttgaaaaaaa aaaaaaaaaa ccccgaaaaa acacaaaaat attttattgt tttaaaaaaa
     8341 aaaaaaaaaa aaaacatgaa cgaaaaccat tcagacgatc aatacggtag agcaagagtt
     8401 caggattcac ctgaattaat tgcttattac aaagaactag aaaaattagg tgctggtgca
     8461 ttatggacgg ttgccaatga tatagaacca tgggaaccaa gaagttcgtc tgtaccaatg
     8521 ctatggaaat atgacaattt acgagaattg gtggtaaaat catccgattt agtaactcct
     8581 gagcaagcag gaagacgcgt ggtttacttg gtaaacgaca aacgaaaaga tgtttctgct
     8641 gctgtaggct ggttatatac tggaattcaa gttacacgtc caggcgaaag cacctcggct
     8701 cacagacata aagcttcggc attgcgtttc atcatggaag gcgaaggcgg atatactgtt
     8761 gtaaatggca ataaaattac gtttgaagta aatgattttg tgataacacc caattctact
     8821 tggcatgaac acggagttgc cgaagacgga aaaacgtgca tttggcaaga cggtttagat
     8881 attcctttag taaatgcgct tgaag
//
LOCUS       DAOOBH010000093         8860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_9727_length_8860_cov_5.752754, whole genome shotgun sequence.
ACCESSION   DAOOBH010000093 DAOOBH010000000
VERSION     DAOOBH010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9727_length_8860_cov_5.752754"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK218_09035"
     CDS             <1..354
                     /locus_tag="PK218_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PUR family DNA/RNA-binding protein"
                     /protein_id="HPW98693.1"
                     /translation="KDEIFSKVLRAGRRTYFFDVRATKADDYYITITESKKFTEEDGS
                     YHFKKHKIYLYKEDFTAFSEILGELTDYVLEHKGEEVISERHQKDFKREFNTETNEES
                     STSEKNFTDIDFDDI"
     gene            complement(617..958)
                     /locus_tag="PK218_09040"
     CDS             complement(617..958)
                     /locus_tag="PK218_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HPW98694.1"
                     /translation="MENSLSWQDFEKIDLRVGTIIDVNDFPNARKPAYQLTIDFGKEM
                     GLKKSSAQITKLYGKKDLIGKQIVAVVNFPKKQIGNFMSECLVLGSVGTDNDIVLLSS
                     DRKVENGLPIG"
     gene            complement(1015..1329)
                     /locus_tag="PK218_09045"
     CDS             complement(1015..1329)
                     /locus_tag="PK218_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98695.1"
                     /translation="MKYKTSLLTVFVLFLFSCATKTKIVSEVKPEVISNEFVTESSES
                     DIVKRGSLLYENNCGNCHSLPKTNEYTLEDWRPILDRMQKEAKISDQEKEDIFFYITR
                     SI"
     gene            complement(1413..2345)
                     /locus_tag="PK218_09050"
     CDS             complement(1413..2345)
                     /locus_tag="PK218_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW98696.1"
                     /translation="MENGIYAKFNTSKGAVLVKLTHDITPGTVGNFVGLAEGQLENDS
                     KAMGKPYYDGLKFHRVIPDFMIQGGCPQGIGTGGPGYKFDDEFHPTLKHDRPGVLAMA
                     NSGPGTNGSQFYITHVPTSWLDGKHTVFGHVIEGQDIVDAVAQGDVLESLEIIRVGED
                     AKNWNAIEAFRTFEGSRVKRMEEAKKQAEEAMEKLAAGFDKTESGLRYKMIQKGSGKQ
                     AEKGKTVSVHYEGSLENGKVFDSSYSRKKPIEFPLGKGHVIEGWDEGITLLQVGDKAR
                     FVIPSYLGYGSSGAGGVIPPDATLVFDVELMDVK"
     gene            2418..2861
                     /locus_tag="PK218_09055"
     CDS             2418..2861
                     /locus_tag="PK218_09055"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HPW98697.1"
                     /translation="MKSIFVITDNDEIIERINKLTPKTKAIWGKMTVDQMLKHTNEAL
                     VVAFGEKELRINFLMKFLGKMMKNKIFNSEFKKSSPTAPEFIFREKYDFDSSKKDLIR
                     NFSRFRVGEQSITLTNHPFWGKMSHEDWNKLMWNHIDHHLRQFGV"
     gene            2901..3701
                     /locus_tag="PK218_09060"
     CDS             2901..3701
                     /locus_tag="PK218_09060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW98698.1"
                     /translation="MKSTNDYLLVNKQTWNNKTEVHVNSDFYNNDAFLKGKSSLNAIE
                     VELLGDISDKKILHLQCHFGQDSLSLARLGATVTGVDLSDNAIEKATSFAKQLDLKAT
                     FICADIYSLPTILDEKFDLIFTSYGTIGWLPDLDKWAKVVSHFLKPNGKFIMADFHPV
                     VWMYDNNFNEVFYSYFNIEPIIEEETGTYAEKNANIEAKTITWNHPISEILNALLTNE
                     LSLNQFNEYDYSPYNCFNATEEFEKGKFRIKHFGNKIPMVYSILATKK"
     gene            3718..4392
                     /gene="trmD"
                     /locus_tag="PK218_09065"
     CDS             3718..4392
                     /gene="trmD"
                     /locus_tag="PK218_09065"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPW98699.1"
                     /translation="MRIDIITILPDLLRSPFEGSIMKRAIEKKLVEVHFHNLRDYTTN
                     KQKSVDDYQYGGSAGMVMNIQPIDDCISHLKSQRNYDEIIYMSPDGETLNQKMANSMS
                     LFENIIILCGHYKGVDQRVRDHFITKEISIGDYVLSGGEIGAIVFCDAIIRLIPGVLS
                     DETSALTDSFQDNLLSGPIYTRPADYKGWKVPEVLLSGNFAKIEQWREEKAFEHTKNR
                     RPDLLE"
     gene            4605..4955
                     /gene="rplS"
                     /locus_tag="PK218_09070"
     CDS             4605..4955
                     /gene="rplS"
                     /locus_tag="PK218_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPW98700.1"
                     /translation="MANLVDFVNNELSTKKDFPEFGAGDTITVYYEIKEGEKTRTQFF
                     KGVVIQRKGSGITETFTIRKMSGAVGVERIFPVNMPALQKVELNQRGKVRRARIYYFR
                     ELTGKKAKIKERRR"
     gene            5106..7328
                     /locus_tag="PK218_09075"
     CDS             5106..7328
                     /locus_tag="PK218_09075"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPW98701.1"
                     /translation="MSDKAKIIYTITDEAPMLATHSFLPIVKAFSKPANISIETRDIS
                     LAGRILSNFPEYLNENQKIADSLSELGKLATTPEANIIKLPNISASVPQLKDAIAELQ
                     SQGYAIPNFPEEATTDEEKNIKAKYAKVLGSAVNPVLREGNSDRRAPKAVKNYAKAHP
                     HSMGKWSSDSKTHVSHMESGDFYGTEQSLTVKNAGSFTIEFTDESGNTKLLKDNSPLK
                     VGEIIDSSVMNMNKLKAFIAEQVADAKEKGVLFSVHLKATMMKISDPLLFGAFVEVYF
                     KDVFTKYADLFHELGVDTKNGLGDVYAKIQGNPKQAEVAAALDEAMQNGPALAMVNSE
                     KGITNLHVPSDVIIDASMPAMIRNSGQMWNADGKSQDTKAIIPDRCYAGLYQATIDFC
                     KKNGALDPKTMGSVPNVGLMAQKAEEYGSHDKTFQLSANGVVNVKDANGTVLMSQKVE
                     AGDIFRMCQVKDAPIQDWVKLAVNRARATGVPAVFWLDDKRAHDRQLIEKVTTYLKDY
                     DTAGLDIRIMNPIDATNFSLERIVKGLDTISVTGNVLRDYLTDLFPILEVGTSAKMLS
                     IVPLMNGGGLFETGAGGSAPKHIEQFIEEGYLRWDSLGEFLALGVSYEHLGNVFHNDK
                     ALVLSETLDEATEKFLETDKSPARKIGSIDNRGSHFYLALYWAQALANQNKDAELKAI
                     FTPISEALTANETKINEELIAAQGKPQNIGGYYHPNFELTDKAMRPSTTFNSILAKLN
                     "
     gene            complement(7357..8034)
                     /locus_tag="PK218_09080"
     CDS             complement(7357..8034)
                     /locus_tag="PK218_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98702.1"
                     /translation="MKAVYALFFLLMISFYGYSQFENPKKTLKIAPVSNPTGQVAPTS
                     SKSITYPSIFDKKDKLLDGVSILNKKPDDTKSVLENDQFESQSKEYTDRMNNKKDGEF
                     HEKFKSDNFLGQFRVATKTISIACRDHEYPDGDLVRIWLNDKIVVESILLDVDFKEVY
                     LELNEGLNKIEIEALNQGDSGPNTAQFTMYDAKKGIITNNKWNLTTGAKAKLIILKEE
                     SLLEEKK"
     gene            8169..>8860
                     /locus_tag="PK218_09085"
     CDS             8169..>8860
                     /locus_tag="PK218_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW98703.1"
                     /translation="MPKKIFLIITIFFNSSLLFSQEKFTLSGIVSDAKNKETLIGVSL
                     YINETKTGISTNEYGFYSITLPKGDYTLNISYIGYQNIEEKISLTQDLKKNFLLSLSS
                     QVLDEVIITDAPKRINIRKPEMSVNRLSIQEIKEMPVILGEVDIIKSILTLPGVTNAG
                     EGQSGFNVRGGGADQNLILLDEATIYNSSHLFGLFSVFNPDAIKDLKLYKGGIPARYG
                     GRLSSVLDIYQK"
BASE COUNT         3133 a         1423 c         1387 g         2917 t
ORIGIN      
        1 aaagatgaaa ttttttctaa agttttaaga gctggaagaa gaacgtattt ctttgatgtt
       61 agagcaacaa aagctgacga ttactacatc actattacag aaagcaaaaa attcacagaa
      121 gaagatggtt cttatcattt caaaaaacac aaaatctatt tatataaaga agactttact
      181 gctttttctg aaattttagg tgaattaacc gattatgtac tagaacacaa aggcgaagaa
      241 gtaatttcag aaagacatca aaaagatttc aaaagagaat ttaacacaga aactaatgag
      301 gaatcatcaa cttctgaaaa gaattttacc gatattgatt ttgacgatat ttaattaaaa
      361 aaaaaacaaa acccaaaact ttcttttggg ttttgttgct ctatacacat gctaaaaata
      421 attgcttttc ttaacaaaca ctcaattctt ttcaattctt ttttcataat tttttggaca
      481 tatattatta tttcaaatta tataaatagt tcaaaaaacg atactgagtt tccaaataaa
      541 actatcttag ttccaataat ttttattctt ttgtcattat tcaatatcta tatggctcta
      601 aaacgccaaa aaaaaactaa ccaataggca atccattctc cacttttcta tcagaactta
      661 ataaaacgat atcgttatct gttccaactg aacccaaaac caaacattca ctcataaagt
      721 tacctatttg ctttttggga aaattaacaa cggcaacgat ttgtttacca atcaaatctt
      781 ttttaccata gagctttgta atttgtgctg aagatttttt tagtcccatt tcttttccaa
      841 aatcgatggt taattgataa gctggttttc tagcgtttgg aaaatcattt acatcaatta
      901 ttgttccaac acgcaggtct attttttcaa aatcttgcca agaaagacta ttttccataa
      961 attgataatt ttatatagtt gaaaaggaat ttcaatttca gatgattata aaatctagat
     1021 acttctagtg atgtaaaaga agatatcttc tttttcttgg tcagaaattt ttgcttcttt
     1081 ttgcattcta tcaagtatgg gtctccaatc ttctagagta tattcatttg ttttaggcaa
     1141 actatgacaa ttaccacaat tattttcata taaaagcgaa cctcttttta cgatgtcact
     1201 ttcagatgat tctgttacaa attcattcga gattacttct ggttttactt ccgaaacgat
     1261 tttagttttt gttgcacaag aaaatagaaa caatacaaaa acagtaagaa gtgatgtttt
     1321 atatttcata attacagttg gttagaaatc aaatataaaa ataaagcaca atagaaatct
     1381 attgcgcttt ataatttaag tatatcttaa tattatttta catccatcaa ttctacatca
     1441 aaaactaatg tagcatcagg aggaataaca cctccagcac ctgagcttcc atatcctaaa
     1501 taagatggaa ttacaaaacg tgctttatcg cctacttgta ataatgtaat tccttcatcc
     1561 caaccttcga taacatgacc ttttcccaaa ggaaattcaa ttggtttttt tcgagaataa
     1621 gaagaatcaa atacttttcc gttttccaaa gaaccttcat aatgaacaga aacagttttt
     1681 cctttttcgg cttgttttcc actacctttc tgaatcattt tataacgtaa gccactttca
     1741 gttttatcaa aaccagcagc taatttttcc attgcttctt cggcttgctt tttggcttct
     1801 tccattcttt ttactcttga accttcaaaa gttctaaacg cttcaatagc attccagttt
     1861 ttggcatcct ctcctactct aataatttct aatgattcta aaacatcacc ttgagcaaca
     1921 gcatctacaa tatcttgacc ttcaataacg tgtccaaaaa cagtatgctt tccatccaac
     1981 caagaagttg gcacatgtgt aatataaaat tgagaaccat ttgttccagg tccagagttt
     2041 gccatagcca aaacgcctgg tctatcatgt tttaatgttg gatgaaattc gtcgtcaaac
     2101 ttatatcctg gtccaccagt tcctattcct tgaggacaac ctccttgaat cataaaatca
     2161 ggaattactc tgtgaaattt tagtccatcg taataaggtt tacccattgc tttggaatca
     2221 ttctccaatt gaccttctgc taaacctaca aaatttccga ctgttcctgg tgttatatcg
     2281 tgtgttaatt ttactaaaac tgcacctttt gaagtgttaa atttagcata tattccattt
     2341 tccatacttt gattttttaa atgaagtgca aatttagtat tttaaattgt aaatttgttt
     2401 aacaaaaact taaaaccatg aaatccattt tcgttataac tgataatgat gaaattatcg
     2461 aacgtatcaa taaattaaca cccaaaacca aagcaatttg gggaaaaatg acagtagatc
     2521 aaatgctaaa acatacaaat gaagctcttg ttgtagcttt tggagaaaaa gaattgcgta
     2581 tcaatttttt gatgaaattc ttaggtaaaa tgatgaaaaa caaaatcttt aattctgagt
     2641 ttaaaaaaag tagtccaaca gcaccagagt ttatcttcag agaaaaatat gattttgata
     2701 gttctaaaaa agacttaatt agaaatttta gtcgctttcg tgttggcgaa caatcaatta
     2761 ctttaaccaa ccatcctttt tgggggaaaa tgtcacacga agattggaat aaattaatgt
     2821 ggaatcatat cgatcatcat ttgagacagt ttggcgtttg agagtagtaa gatttgagta
     2881 ttaagaatgt aaaactaaaa atgaaaagca ctaacgatta cttattggtc aacaaacaaa
     2941 cttggaacaa caaaacggag gttcatgtga attctgattt ttacaataac gacgcttttt
     3001 tgaaagggaa atcaagctta aatgcaattg aagtagaact tcttggagac atttctgata
     3061 aaaaaatatt acatttgcaa tgtcattttg gacaagatag tttgtcattg gcacgtttgg
     3121 gtgcaacagt aactggtgtt gatttatcgg ataacgccat tgaaaaagca acttcttttg
     3181 caaagcaatt agacctaaaa gcaactttta tttgtgctga catttatagt ttgcctacta
     3241 ttttagatga aaaatttgac cttattttca ccagttatgg tacaattggt tggttacccg
     3301 atttagataa atgggcaaaa gttgtatcgc attttctaaa acctaatgga aaatttatta
     3361 tggcagattt tcatcctgta gtttggatgt atgataataa ttttaacgaa gttttttata
     3421 gttacttcaa catcgaacca atcattgaag aagaaacagg aacgtatgcc gaaaaaaatg
     3481 ctaacataga agccaaaacc ataacttgga atcatcccat ttcggaaatc ttaaatgcgc
     3541 tgctaactaa cgaattatca ttaaatcaat ttaacgaata tgattattca ccttataatt
     3601 gtttcaatgc gactgaagaa tttgaaaaag gaaaattcag aataaaacac tttggaaata
     3661 aaattccgat ggtgtattcg attttagcaa ccaaaaagta aaacatatct ttgcactatg
     3721 cgaatagaca tcattaccat tttacctgat ttattgcgaa gtccatttga aggctcgatt
     3781 atgaaacgtg ccattgaaaa aaaattagta gaagttcatt ttcacaattt acgagattat
     3841 acaaccaaca aacagaaaag tgtggacgat tatcaatatg gcggtagcgc aggaatggtg
     3901 atgaatattc aacccattga tgattgtatt tcgcatttga aatcacaaag aaattatgac
     3961 gaaatcatct atatgtctcc agatggtgaa acattgaacc agaaaatggc gaattccatg
     4021 tcgctgtttg aaaatattat cattctttgc ggacattata aaggtgttga ccaaagggtt
     4081 cgcgaccatt ttatcaccaa agaaatttct attggtgatt atgttctttc tggtggcgaa
     4141 attggtgcta tcgttttttg tgatgctatt attcgattaa ttccaggtgt tttatcagac
     4201 gaaacttcag cattgaccga cagttttcaa gacaatttac tttcaggacc aatttataca
     4261 cgaccagcag attacaaagg ttggaaagtt ccagaagttt tattgagcgg aaactttgcc
     4321 aaaattgagc aatggcgcga agaaaaagct tttgaacaca ctaaaaatag acgaccggat
     4381 cttctcgaat aaattccaaa atattaaaat tccaaatttc aaagcagtaa atatttctat
     4441 ttatttagtt atcaaatttt tataatacta tttcttcttt aaaaaaaatt ggaacttgga
     4501 atttgaaact tgaaattttt aattatattt gcacccacat ttgactaacc tctggcgata
     4561 tgcgtgaatg ttactcagat ttaaaccata atttagcaat caaaatggca aatttagtag
     4621 atttcgttaa caacgaattg tcaacaaaaa aagatttccc tgaattcgga gctggtgata
     4681 ctatcaccgt ttattacgaa atcaaagagg gtgaaaaaac aagaactcag ttttttaaag
     4741 gagttgttat tcaaagaaaa ggttcaggaa taactgaaac ttttacaatt cgtaaaatgt
     4801 ctggtgctgt tggtgtagag cgtatcttcc cagtaaacat gccagcttta caaaaagttg
     4861 aattaaacca aagaggtaaa gttcgtagag cacgtattta ctacttcaga gaacttacag
     4921 gtaaaaaagc aaaaattaaa gaaagaagaa gataatttct ttttcaatct aaagtataaa
     4981 agagttgtag taaatgctat aactcttttt ttattgcagc tattcaacat ggaagtttta
     5041 aaatagctcg taattcttta tatttgcgag aatgtaaatt tcacaacact tttatacaaa
     5101 attatatgtc tgataaagca aaaattattt atacaattac tgatgaagct ccaatgctag
     5161 ctacacattc ctttctacca attgtaaaag cattctcaaa accagcaaac atatcaatag
     5221 aaaccagaga tatttcatta gcgggaagaa ttttatctaa ctttccagaa tatctaaatg
     5281 aaaaccaaaa aattgccgat tctttatccg agttaggaaa actagctaca actccagaag
     5341 ctaatattat aaaattacca aacatatcag catcagttcc tcagttaaaa gacgccattg
     5401 ctgaattgca atcacaaggt tacgctattc caaattttcc tgaagaagca actactgatg
     5461 aagaaaaaaa catcaaagca aaatatgcca aagtattagg ctcggctgta aatccggttt
     5521 tgcgtgaagg aaactccgac agacgcgcac caaaagcagt aaaaaattac gccaaagcac
     5581 atcctcattc tatgggcaaa tggtcttcgg attcaaaaac acatgtttct cacatggaaa
     5641 gtggtgattt ttatggcaca gaacaatctc ttactgtaaa aaatgctgga agtttcacaa
     5701 ttgaatttac agacgaaagt ggaaatacta aattattaaa agacaattct ccattaaaag
     5761 ttggagaaat cattgatagt tcagtaatga acatgaataa gttaaaagct tttattgcag
     5821 aacaagttgc cgatgcaaaa gaaaaaggtg tcttattctc agtacacttg aaagccacaa
     5881 tgatgaaaat ttcagatcca ttactttttg gtgcttttgt agaagtttat ttcaaagatg
     5941 tttttacaaa atatgctgat ttattccatg aattaggcgt tgatactaaa aatggtttgg
     6001 gtgatgttta tgccaaaatt cagggtaatc ctaaacaagc cgaagtagca gctgcattag
     6061 atgaagctat gcaaaatggt ccggctttag caatggttaa ttcagaaaaa ggaattacta
     6121 atcttcatgt gccatccgat gtcattattg atgcatcaat gccagcaatg atacgaaatt
     6181 cgggtcaaat gtggaatgcc gatgggaaat ctcaagatac caaggcaatt attcctgacc
     6241 gttgttatgc aggattatac caagcaacaa ttgatttctg taaaaagaat ggtgcattag
     6301 acccaaaaac aatgggaagc gttcctaatg ttggattgat ggcgcaaaaa gcagaagaat
     6361 atggctcaca cgataaaaca ttccaacttt cggcaaatgg agttgtaaat gttaaagatg
     6421 caaatggcac tgtattaatg tcacaaaaag tagaggctgg cgatattttt agaatgtgtc
     6481 aagtgaaaga cgctccgatt caagattggg taaaattagc tgtaaatcgc gctagagcaa
     6541 ctggtgttcc tgctgttttt tggcttgatg acaaacgagc acatgataga caattaattg
     6601 aaaaagttac tacttattta aaagattatg atacagctgg acttgatatt cgcataatga
     6661 atccaataga tgcaacaaat ttttctctag aaagaattgt aaaaggtttg gatactattt
     6721 cggtgactgg aaatgtttta cgcgactatt taacggattt attcccaatt ttagaagttg
     6781 gaacttcagc aaaaatgtta tcaattgttc ctttaatgaa tggtggtgga ttatttgaaa
     6841 ctggagctgg tggttccgct ccaaaacata ttgaacaatt tattgaagaa ggttatttgc
     6901 gttgggattc gttgggagaa tttttagcac taggtgtttc ttatgaacat ttgggtaatg
     6961 ttttccacaa tgataaagca ttggttttat cggaaacatt agatgaagcc acagaaaaat
     7021 tcttggaaac cgataaatct ccagcgcgca aaattggtag cattgacaat agaggctctc
     7081 atttttattt ggctttatat tgggcacaag cattagcaaa ccaaaataaa gatgccgagt
     7141 taaaagctat ctttactcct atttctgaag ctttaactgc aaatgaaact aaaattaacg
     7201 aagagttaat cgctgcacaa ggaaaacctc aaaacattgg tggttattat catcctaatt
     7261 ttgaattaac cgataaagcg atgcgaccaa gtacaacttt caattcgata ttagcaaagt
     7321 taaactaatt aaaaagcgac caattggtcg cttttttcac tttttttctt caagcaaact
     7381 ctcttctttt aaaattatca acttcgcttt tgcaccagtg gttaaattcc atttattatt
     7441 agtaataatg cctttttttg catcatacat agtgaattgt gcagtattag gaccagaatc
     7501 tccttgattt aacgcttcaa tttctatttt atttaaccct tcattcagtt ctaaataaac
     7561 ctctttaaag tcaacatcta ataaaattga ttccactaca attttatcat tcagccaaat
     7621 tcgaaccaaa tcaccatcag gatattcatg atcacgacag gcaatactaa tggttttagt
     7681 agcaactcta aattgtccca aaaaattatc gctcttaaat ttttcatgaa actcaccgtc
     7741 ctttttgttg ttcattctat cggtatattc tttggattga ctctcaaatt ggtcgttttc
     7801 cagtacactt ttagtatcat ctggtttctt atttaaaata gaaacaccgt ccaataattt
     7861 atctttttta tcaaaaatac ttggataagt tatcgatttt gaagaagtag gtgcaacttg
     7921 acctgttgga ttggaaacag gagcaatttt caaggttttc tttggatttt caaactgaga
     7981 atatccataa aaacttatca ttaatagaaa aaacaaagca taaactgctt tcataagtag
     8041 aaatttattt ttgtaaaagt atttaaaata gtaaacttta ctaggtttaa ttaaccaaat
     8101 attaacaact gattattagt atttaggaaa aattattaag atttttgtaa aaataaacag
     8161 caaattctat gcctaaaaaa atatttttaa tcattactat attctttaat tcttcattat
     8221 tattttcaca agagaaattt acgttaagtg gaatcgtatc tgacgcaaaa aataaagaaa
     8281 cattaattgg tgtttcgctt tatattaatg aaacaaagac tggaatttca accaatgaat
     8341 atggttttta ctcaataacc ttacccaaag gtgactatac tttaaatatc agttatattg
     8401 gttatcaaaa tattgaggaa aaaattagtt taactcaaga tttgaaaaaa aactttttac
     8461 tttcattaag tagtcaagtt ctagatgaag taattataac ggatgctcca aaaaggataa
     8521 atattcgaaa acctgaaatg agtgtgaaca gactttcgat acaagaaata aaagaaatgc
     8581 ccgttattct tggtgaagtt gatatcatca agtccatttt aactttgcca ggtgttacaa
     8641 acgctggaga agggcaatcg ggttttaatg ttcgtggtgg tggagccgat caaaatttaa
     8701 ttttgttaga tgaagctaca atttataatt cttcccattt gtttggatta ttttcggtat
     8761 ttaatcctga tgctataaaa gatttaaaat tatacaaagg tggtattcca gctcgatatg
     8821 gcggaaggtt atcttcggtt ttagacattt atcaaaaaga
//
LOCUS       DAOOBH010000094         8691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_9957_length_8691_cov_5.195229, whole genome shotgun sequence.
ACCESSION   DAOOBH010000094 DAOOBH010000000
VERSION     DAOOBH010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9957_length_8691_cov_5.195229"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..360)
                     /locus_tag="PK218_09090"
     CDS             complement(31..360)
                     /locus_tag="PK218_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98704.1"
                     /translation="MYIIVLKYLVPKKIIGLTIFPFIIVKDKKLKLDKVFINHEKIHL
                     RQQLELLILPFFVWYFLEFFFRFMICLNWSKSYRSISFEKEAYCNEMNFNYLEKRTLY
                     RFLKYII"
     gene            389..571
                     /locus_tag="PK218_09095"
     CDS             389..571
                     /locus_tag="PK218_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HPW98705.1"
                     /translation="MDDQNTENKLIEGEDYYLTSEGYRCFTEKYHLKRGYCCKSGCRH
                     CPYGFDKKTGTIKPKK"
     gene            598..2574
                     /locus_tag="PK218_09100"
     CDS             598..2574
                     /locus_tag="PK218_09100"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPW98706.1"
                     /translation="MTFKEQILQGIPSILPQPKPYEKEINHAPKRKEILSDDEKKLAL
                     QNALRYFEPKHHAELIPEFKAELETYGRIYMYRLRPDYKMYARPISEYPGKSEQAKAI
                     MLMIQNNLDYAVAQHPHELITYGGNGAVFQNWAQYLLTMQYLAEMTDEQTLVMYSGHP
                     MGLFPSHKEAPRVIVTNGMVIPNYSKPDDWEKMNALGVSQYGQMTAGSYMYIGPQGIV
                     HGTTITVLNGFRKIKKSPKGGLFVTSGLGGMSGAQPKAGNIAGCITVCAEVNPKITHI
                     RHSQGWINEIIENIDELVPRVKKALENKEVVSIAYLGNVVDVWERFDQENVHIDLGSD
                     QTSLHNPWAGGYYPIGHSFEESNEMMANNPEKFKKEVQKTLRRHATAINKHTAKGTYF
                     FDYGNAFLLEASRAGADIMAENRIDFKYPSYVQDIMGPMCFDYGFGPFRWVCATGNPE
                     DLAKTDKIACDVLEEMMKNSPEEIQQQMADNIQWIKGAQENKLVVGSQARILYADAEG
                     RIKIAEAFNQTIAKGEIGYVILGRDHHDVSGTDSPYRETSNIYDGSRFTADMAIQNVI
                     GDSFRGATWVSIHNGGGVGWGEVINGGFGMVLDGTKEASKRLESMLFWDVNNGISRRS
                     WARNEGAIFAIKRAMETQPLLKVTIPNIVDESLL"
     gene            2639..3172
                     /locus_tag="PK218_09105"
     CDS             2639..3172
                     /locus_tag="PK218_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HPW98707.1"
                     /translation="MKNIKFLPLLLLFVITSCSSIKVNQDYDKNVSFDGYKTYAYHKS
                     GIDKVEISDLDKKRILRSIDEVMANKGFTKSENPDLLINFFTKEREQVNVNQFNAGWG
                     YGWGWGWNPWMWGGNTTISRYSEGTLTIDIIDAKKKELIWQGEGEGVLTKNTEKKEEN
                     VKEFVTKILAQYPPQKK"
     gene            complement(3206..>4492)
                     /locus_tag="PK218_09110"
     CDS             complement(3206..>4492)
                     /locus_tag="PK218_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPW98708.1"
                     /translation="HTSGVRMEWYRDDLEEAQSRDPFPVLIQQLLDAGFTQQEIDHEL
                     TSASAVVKKDFEKAQQAEDPKPEDLFTHDFAPTLITKEVGERNPAREEKVVMVDCALF
                     AVEEIMKKHPESLLYGQDVGGRLGGVFREAATLAQKFGDERVFNTPIQEAFIVGSTVG
                     MSAVGLKPIVEVQFADYIWPGLNQLFTEVSRSCYLSNGKWPVSMILRVPIGAYGSGGP
                     YHSSSVESVLTNIRGIKIAYPSNGADLKGLLKAAYYDPNPVVFLEHKGLYWSKVKGTD
                     AARVNEPSEDYMLPFGKANIVQEIWEQETEETISVITYGMGVHWALNASADIKNQVEI
                     IDLRTLYPLDEEAILKSVRKTKKCLVVTEEPTNNSFARSLAGLIQEKCFRNLDAPVMV
                     IGSENMPAIPLNSTLEFTMIPNVDKVKVKIEELLNY"
     assembly_gap    4493..4592
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4797..5852)
                     /locus_tag="PK218_09115"
     CDS             complement(4797..5852)
                     /locus_tag="PK218_09115"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPW98709.1"
                     /translation="MTSEIKISKVKESKVTQVDLDNVTMGFNFTDHMFVCDYTNGEWQ
                     NPRIEALNLIPTHPAAMALHYGQAIFEGMKATKGQDGTPLLFRPDQNAKRLNFSAYRM
                     GMPQFPEDMFVEALKQFTAIEKDWIPTKEGSALYLRPFMYADEPFIGMRAATSYKFII
                     LGSPAGPFFSKKIKLFAENKYVRAVNGGTGEAKAAGNYAAAIRPTEYAKAKGYDQVLW
                     LDAQDFEYIQEVGTMNIFFKIDGKFITPDLSGSILAGITRMSVIDLLKDKGFEVTERP
                     IKLSEIKEASANGTLEEAFGVGTAVGIAMIEEIGNDDFTLKLPAENPVSNMVNDTLNA
                     IKVQKAEDKFGWIVEAK"
     gene            complement(5868..7154)
                     /locus_tag="PK218_09120"
     CDS             complement(5868..7154)
                     /locus_tag="PK218_09120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPW98710.1"
                     /translation="MITEFKMPKMGESISEATIISWLKNIGDFVDAEETILEVATDKV
                     DSEVPSPVSGTITEIRFQKDDVVAVGTVLALIDADGKQEAKSTKQEVVSTKVETQPEV
                     AKQSNIEFKQLKSNPDAFLSPLIVSIAQKENLSLEEVQSIPGSGTDGRIQKSDVFNYL
                     KNRKYPLKSIPQPTTQNLQSTTFPKPKINYVEGKDSIVEMDRMRKMIADHMVYSKQTS
                     PHVTSYIEVDVTNLVDWRNANKDKFQEKYNEKLTFTPIFVQAVAKAIEDFPLINVSVD
                     EKKIYVHKDINIGMATALPSGNLIVPVVKNANEKDILALAKDVNQLADASRNNKLKPE
                     QIQGSTFTISNVGTFGSLMGTPIINQPEVAILALGIIKKRPEVITTEKGDEIAIRSMM
                     YLSLSFDHRVVDGFLGGSFLRKVGDYLEQFDTNTTI"
     gene            complement(7202..7798)
                     /locus_tag="PK218_09125"
     CDS             complement(7202..7798)
                     /locus_tag="PK218_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HPW98711.1"
                     /translation="MIYEFKGFIPVIDESSFIHPQAAVTGNVIIGKNVYVGPGAAIRG
                     DWGEIIIEDGCNVQENCTIHMFPGKSMVLKAGAHIGHGAIIHGANIGANCLVGMNAVI
                     MDDVNVGDECIIGALAFVKAGMQIPNRKMVVGNPATIIKDVSDGMIDWKTKGTALYQK
                     LPSECYESLKAVEPLREIPSNRPTQEAFYKTLEEFKNK"
     gene            complement(7882..8634)
                     /locus_tag="PK218_09130"
     CDS             complement(7882..8634)
                     /locus_tag="PK218_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPW98712.1"
                     /translation="MSQAYVKQEIKNNIATIEFFHPEHNSLPSDILAKLANTITEVGN
                     NNEVKVIILKSGGDRTFCAGASFQELISINDAATGKVFFSGFANVINAMRKCPKFIIG
                     RIQGKTVGGGVGIAAATDYCMATKFAAIKLSELNVGIGPFVVAPAIERKMGVSAMSQI
                     AIDANTFYEANWAKQKGLFANVYESIEELDTEVQKFAEHLCTYNPEAMQEMKKVFWRG
                     TEDWDNLLTERAAISGRLVLSDFTKETLKKFK"
BASE COUNT         2652 a         1534 c         1419 g         2986 t
ORIGIN      
        1 atattcaaaa aattaatttt taaaaaaaaa tcaaattata tacttcaaaa accgatataa
       61 agttcttttt tccaaataat taaaattcat ttcattacaa tatgcttctt tttcaaagga
      121 aattgaacga tatgatttac tccaatttaa acaaatcatg aatcgaaaaa aaaattccaa
      181 aaaataccaa acaaagaaag gaagaattaa taattctaat tgttgtctca aatgaatttt
      241 ttcgtgatta ataaaaactt tgtccagctt taattttttg tctttgacta taataaatgg
      301 aaaaattgtc aaaccaataa tttttttggg tactaaatat tttaaaacaa taatatacat
      361 tcttctaaaa gtgattaaat ttgcatttat ggatgaccaa aatacagaaa ataaactaat
      421 agaaggtgaa gattattatt taacatccga gggttatcga tgttttaccg aaaaatacca
      481 tttaaaacgt ggttattgtt gcaaaagcgg atgcagacat tgtccttacg gatttgacaa
      541 aaaaactgga accataaaac caaaaaaata aagcaactaa ctataacaca cactaaaatg
      601 acatttaaag agcaaattct tcaaggaatt ccgtctattt tacctcaacc aaaaccttac
      661 gaaaaggaaa ttaaccacgc accgaaaaga aaagagatac tttctgatga cgaaaaaaaa
      721 ttagcactac aaaatgcttt acgttatttc gaaccaaaac atcacgcaga attaattcct
      781 gaatttaaag cggaacttga aacttacggt agaatttata tgtaccgttt gcgtcctgat
      841 tataaaatgt atgcaagacc aatttccgaa tatcctggaa aaagtgaaca agctaaggca
      901 atcatgctga tgattcaaaa taacttagat tatgcggttg ctcaacatcc acatgaattg
      961 attacttatg gtggaaatgg tgctgttttt caaaattggg cacaatactt attgaccatg
     1021 caatatcttg ctgaaatgac ggatgaacaa acattagtga tgtattctgg tcatccgatg
     1081 ggattatttc caagtcataa agaagctcca agagttatag ttaccaatgg aatggttatc
     1141 cctaattatt caaaacctga tgattgggaa aaaatgaacg ctttaggtgt ttcgcaatac
     1201 ggtcaaatga cagctggaag ttatatgtat attggtccgc aaggaattgt acatggaaca
     1261 actattactg ttttaaacgg attcagaaaa atcaaaaaat caccaaaagg tggattattt
     1321 gtgacttctg gattaggtgg aatgtctggt gctcaaccaa aagctggaaa tattgcaggt
     1381 tgcataactg tttgtgcaga agtaaatcca aaaattacgc atattcgtca ttcacaaggt
     1441 tggataaatg aaattataga aaacattgac gaattggttc caagagttaa aaaagcttta
     1501 gaaaataaag aagttgtgtc tatagcttat ttaggaaatg tcgttgatgt ttgggaacga
     1561 tttgaccaag aaaatgtcca tatcgattta ggttccgacc aaacatcgtt gcacaatcct
     1621 tgggctggag gttattatcc aattggacat tcttttgaag aatccaatga aatgatggcc
     1681 aataacccag agaaattcaa aaaagaagtt caaaaaacgt tacgtcgaca tgctactgca
     1741 atcaacaaac acactgcgaa aggaacttat ttcttcgatt acggaaatgc ctttttacta
     1801 gaagcttctc gtgctggagc agatattatg gcagaaaatc gtatcgattt taaatatcca
     1861 agttatgttc aagacattat gggaccaatg tgtttcgatt acggatttgg accatttcgt
     1921 tgggtttgtg ctacaggaaa tccagaagat ctagcaaaaa cagataaaat tgcttgcgat
     1981 gttttagaag aaatgatgaa aaattctcca gaagaaattc agcaacaaat ggcagataat
     2041 attcaatgga taaaaggcgc acaagaaaac aaattagtgg ttggttcgca agccagaata
     2101 ctttatgccg atgccgaagg aagaattaaa atagcagaag catttaacca aactatagca
     2161 aaaggtgaaa taggatatgt tattttaggc cgagatcatc atgacgtttc aggaacagat
     2221 tctccataca gagaaacttc aaatatctat gatggttctc gttttactgc cgatatggca
     2281 attcaaaatg tcattggcga tagttttcgt ggtgcaactt gggtttctat tcacaatggt
     2341 ggtggcgttg gttggggtga agtaattaat ggaggttttg gtatggttct tgatggaact
     2401 aaagaagctt caaaacgctt agaatcaatg ctcttttggg atgtaaacaa cggaatttcg
     2461 agaagaagtt gggcaagaaa cgaaggcgca atttttgcaa taaaaagagc gatggaaacg
     2521 caacctttgt tgaaagttac catcccaaat atagtagacg aatcactatt ataatttttt
     2581 taagttttgt agtttttgtt atatctgaat tttaaaatta taatataaat taatagctat
     2641 gaaaaacatc aaattccttc cgctactttt actttttgtt attacttctt gtagttcaat
     2701 taaagtaaat caagactatg ataaaaatgt atcctttgat gggtacaaaa cttatgctta
     2761 ccataaaagt ggtattgata aagtagaaat atcagattta gataaaaaac gaattcttcg
     2821 ctctatagat gaagttatgg caaataaagg atttacaaaa tctgaaaacc ctgatttgtt
     2881 aattaatttt ttcacgaaag aaagagagca agtcaacgtt aaccaattca acgctggttg
     2941 gggttatggc tggggttggg gttggaatcc gtggatgtgg ggtggaaaca caaccatttc
     3001 aagatatagt gaaggcactt tgaccattga tataattgat gctaagaaga aagaattgat
     3061 ttggcaaggc gaaggtgaag gtgttttaac caaaaacact gaaaagaaag aagaaaatgt
     3121 aaaagagttt gtcacaaaaa ttctggctca atatccgcca caaaagaaat aaataaaaaa
     3181 acctccaaat tggaggtttt tttatttaat agtttaacaa ctcttcaatc ttaactttta
     3241 ctttatctac atttggaatc atggtgaatt ctaaagtact gtttaaagga attgctggca
     3301 tattttcaga accgataacc atgactggtg catccaaatt tctgaagcat ttctcttgga
     3361 ttaatcctgc caaacttctg gcaaaagaat tattcgttgg ttcttcggtt accactaaac
     3421 attttttagt ttttcttact gattttaaaa tcgcttcttc gtctaaagga tacaatgttc
     3481 ttaaatcgat aatttctact tgatttttga tgtctgctga agcattcaaa gcccaatgaa
     3541 ctcccattcc gtaagtaatt acagaaatag tttcttctgt ttcttgttcc caaatttctt
     3601 gtacaatatt cgcttttccg aaaggcaaca tataatcttc gctaggttca ttgactctag
     3661 ctgcatctgt tcctttgact ttgctccaat acaacccttt gtgctctaaa aacactactg
     3721 gatttggatc gtaataagcc gctttcaaca aaccttttaa atcggcacca ttactcggat
     3781 aagcgatttt tattcctcta atattcgtta aaacactttc aaccgatgaa gaatgatatg
     3841 gcccgccact cccgtaagct ccaatgggaa cacgcaaaat catgcttact ggccattttc
     3901 cattcgataa ataacaggaa cgactgactt ctgtaaacaa ttgatttaaa ccaggccaaa
     3961 tataatcagc aaattgtacc tcaacaatag gttttaaacc aaccgctgac attccaaccg
     4021 ttgaacccac aataaatgct tcttggattg gtgtattgaa aaccctttcg tctccgaatt
     4081 tttgcgctaa agtggctgct tctctaaaaa ctccacctaa tcgtccacca acatcttgtc
     4141 cgtataataa actttctgga tgttttttca tgatttcttc gactgcaaac aaagcacaat
     4201 ctaccataac cactttttct tcacgtgctg gattacgttc tccaacttct ttcgtgatta
     4261 gtgttggagc aaaatcgtgc gtaaataaat cttctggttt tggatcttct gcttgttgag
     4321 ctttttcgaa atctttttta accacagctg aagctgaagt caattcatgg tctatttctt
     4381 gttgggtgaa acctgcatct aataattgtt ggattagaac tggaaatgga tcacgagatt
     4441 gagcttcttc caaatcatca cgataccatt ccattcgcac accagatgta tgnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcaatctg aatcgcttca tgaccacgag
     4621 aagttgcatg cacgtatttg gaaactattt tgaaattttc ttcatacaat tcggccatgg
     4681 cttttgctgt tgccatagtt gaaaatgctt tttgtaatat gtttttgtct aacattttgt
     4741 ttttatattt aacccttcaa gggtttaaaa cccttgaagg gttcttttaa tctaatctat
     4801 tttgcttcta caatccaacc gaatttatct tcagcctttt gaacttttat ggcattcaac
     4861 gtatcattca ccatatttga aacaggattt tctgctggta attttaaagt gaaatcatca
     4921 tttccgattt cttcaatcat ggcaattcca actgctgttc ccacaccaaa agcttcttct
     4981 aaagttccgt tggctgatgc ttctttgatt tcagataatt tgataggtct ttcagttact
     5041 tcaaaacctt tgtcttttaa taaatcaatt acactcattc ttgtaattcc tgctaaaatc
     5101 gaacctgata aatctggagt aatgaatttt ccatctattt tgaagaaaat attcatcgtt
     5161 cctacttctt ggatgtattc aaaatcttgt gcatctaacc acaatacttg gtcgtaccct
     5221 tttgcttttg catattctgt gggacgaatc gctgctgcat aatttcctgc tgcttttgct
     5281 tctccagttc caccgtttac agcacgaacg tatttgtttt cagcaaaaag tttaattttt
     5341 ttactgaaaa atggtcctgc aggtgaacct aaaattataa atttataact ggttgcagct
     5401 ctcattccaa taaaaggttc gtctgcatac ataaaaggtc ttaaatataa agcacttcct
     5461 tctttggttg gaatccaatc tttttcaatc gctgtaaatt gtttcaaggc ttccacaaac
     5521 atatcttctg gaaattgtgg cattcccatt ctatatgcac taaaatttaa acgttttgca
     5581 ttttggtcag gacgaaataa caatggtgtt ccgtcttgtc cttttgtagc tttcatacct
     5641 tcaaaaatag cttgtccgta atgcaaagcc attgctgctg gatgtgttgg tattaaattt
     5701 aaagcttcaa ttcttggatt ttgccattca ccattagtat aatcgcaaac aaacatatga
     5761 tctgtaaagt tgaatcccat agtaacatta tccaaatcta cttgggtaac ttttgattct
     5821 ttgactttac ttatttttat ttctgatgtc atattctctg tcatgtttta tatcgttgta
     5881 tttgtatcaa attgttctaa ataatctccc acttttcgca agaatgaacc tcctaaaaat
     5941 ccatcaacaa ctctgtggtc aaatgaaagg gaaaggtaca tcatacttct aatggcaatt
     6001 tcatctcctt tttcagttgt gataacttcg ggtctttttt tgatgattcc taaagctaaa
     6061 atggcaactt ctggttggtt gataattggt gttcccatta aacttccaaa agttcctacg
     6121 tttgaaatcg taaaagtact tccttgaatt tgttctggtt ttagtttgtt atttcttgaa
     6181 gcatcagcta gttgatttac atcttttgct aaagctagta tatctttttc gttggcattt
     6241 tttacaactg gaacaattag attgccactt ggtaaagccg ttgccattcc aatattgata
     6301 tctttatgca cataaatttt cttctcatcc acagatacat tgattaaagg aaaatcttca
     6361 attgctttgg ctactgcttg aacaaatatt ggtgtaaaag ttagtttttc gttgtatttt
     6421 tcttgaaatt tatctttgtt agcatttctc caatctacca aattcgtaac atctacttct
     6481 atgtatgaag tcacgtgtgg agaagtttgc tttgaataga ccatatgatc cgcaatcatt
     6541 ttgcgcattc tatccatttc tactatgctg tcttttcctt ctacataatt gattttaggt
     6601 tttggaaagg ttgtagattg taggttttgg gttgtaggtt gaggaatact ttttaaagga
     6661 tattttctat tttttaaata attgaaaaca tcactttttt gaattctccc atcagttcct
     6721 gaacctggaa tactctgtac ttcttctaac gaaagatttt ctttttgagc tatacttaca
     6781 atcaaaggtg aaagaaatgc gtcaggattg cttttaagtt gcttaaactc aatgtttgat
     6841 tgcttagcaa cttctggttg agtttcaact ttagtgctaa caacttcttg ctttgtactt
     6901 ttagcttctt gttttccatc agcatcaatt agagctaaaa cagttccaac cgcaacaaca
     6961 tcgtcttttt gaaaacgaat ttcggtgatt gttcctgaaa ctggtgaagg cacttcgcta
     7021 tcaactttat cggttgcaac ttctaaaatt gtttcttctg cgtccacaaa atctcctatg
     7081 tttttcaacc aagaaattat cgtagcttcg gatatacttt cacccatttt gggcattttg
     7141 aattctgtta tcatttctat aaatacctac aaggttttga aaaccttgca ggatttgtaa
     7201 attacttatt cttaaattct tctaatgttt tataaaacgc ttcttgtgtt ggacgattag
     7261 atggaatttc tcttaacggt tctactgctt ttaaactctc ataacattct gatggaagtt
     7321 tttgatacag ggctgttcct tttgttttcc aatcaatcat tccgtctgaa acatctttta
     7381 taattgtggc tggatttcca actaccattt tacgatttgg gatttgcatt cctgctttta
     7441 cgaaagctaa ggctccaatt atacattcat ctccgacatt cacatcatcc atgattactg
     7501 cgttcattcc aaccaaacaa ttagctccaa tgttagctcc gtgaatgata gctccgtgac
     7561 caatatgtgc tcctgctttt aaaaccatag atttcccagg aaacatgtga atggtacaat
     7621 tttcttgaac attgcaaccg tcttcaataa tgatttcacc ccaatctcca cgaatggctg
     7681 cacctggacc aacatatacg ttttttccaa taatcacatt acccgtaaca gctgcttgag
     7741 gatgaatgaa actactttcg tctattactg ggatgaaccc tttgaattcg tatatcattt
     7801 tttcttttag ataatagatg atagacggtt agaaaaaaga cttacctgtc tattatctct
     7861 tcgtctttta tttttttgtc tttatttaaa cttcttcaat gtttcttttg taaaatcaga
     7921 tagaactaat cttccgctga tggctgctct ttcggttaaa aggttgtccc aatcttctgt
     7981 tcctctccag aatacttttt tcatttcctg catcgcttct ggattgtatg tgcataaatg
     8041 ttcagcgaat ttttgaactt ctgtatctaa ttcttctatg ctttcataca catttgcgaa
     8101 taatcctttt tgttttgccc aatttgcttc gtaaaaagtg ttggcatcaa ttgctatttg
     8161 ggacatggct gaaactccca tttttctttc gattgctggt gctactacga atggtccaat
     8221 acctacattt agttcagata attttatggc cgcgaatttt gttgccatgc aataatcagt
     8281 tgctgctgcg attccaactc cacctccaac ggtttttcct tgaattcttc ctatgatgaa
     8341 ttttggacat tttcgcatcg cattgattac gtttgcaaat cctgaaaaga atacttttcc
     8401 ggttgctgca tcgtttatgg aaattaattc ttgaaaactt gctcctgcac agaaagttct
     8461 gtcaccacca ctttttagga tgattacttt tacttcgttg ttgtttccaa cttcggttat
     8521 tgtgttagct aatttggcta atatatcgct tggtaatgaa ttgtgttctg gatgaaagaa
     8581 ctcgattgtg gcgatgttgt tttttatttc ttgttttacg tatgcttgtg acatgttttt
     8641 aattttaacc gcaaagtgcg taaggtttta cgcaaggttc gcaatgattt t
//
LOCUS       DAOOBH010000095         8633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_10048_length_8633_cov_6.657146, whole genome shotgun sequence.
ACCESSION   DAOOBH010000095 DAOOBH010000000
VERSION     DAOOBH010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10048_length_8633_cov_6.657146"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PK218_09135"
     CDS             <1..834
                     /locus_tag="PK218_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW98713.1"
                     /translation="QLETDEIIQEKDQLEDKNRNLLYFFFGSVGLFGFLYYLRTQRAK
                     NRELIYKQAQQRANEEIFNLMMSQQAIIDESRTQEKQKLARDLHDGVLGRMFGLRLSL
                     DSVNRKNDEASLLKRLQIIDELKTIEQDIREISHDLNREKQVLINNFVSIVNNLIEEQ
                     KEKHHAKVSFFLDTSIHWDKIASAVKINLYRILQESLQNINKYANAKNINIGFVKNED
                     TIIFKISDDGIGFDANKKTKGIGTQNMVARTKECKGTIDIQSEIGEGTTITITIPTEN
                     K"
     gene            856..1524
                     /locus_tag="PK218_09140"
     CDS             856..1524
                     /locus_tag="PK218_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW98714.1"
                     /translation="MENPINVLIVDDHPFIIEAYKNALNKYSLQGYEFNVTQAADCKS
                     GYELIVSDDNNYNLAMFDVSMPEYVEKNIRSGMDLAKLMQNENPSCKVMLLTMHVELD
                     RINAIIKEINPNSLLIKNDINFDELIFALDKTLKGDKYYSETIIKLLGQGEFIKVEFD
                     AMDKRILELMAKEYETILMARHLPLTPVVIEVRKKRIREVFNLPDATDAEIVEQARAK
                     QIIE"
     gene            complement(1554..2603)
                     /gene="thiL"
                     /locus_tag="PK218_09145"
     CDS             complement(1554..2603)
                     /gene="thiL"
                     /locus_tag="PK218_09145"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPW98715.1"
                     /translation="MLEDKNPQKTSLSQLGEFGLIDHLTKDFDVKQPSTIKSIGDDAA
                     VLDFAAKKVVVSTDLLIEGVHFDLAYMPLRHLGYKAVVVNLSDIAAMNAKPTQITVSV
                     AVSNRFPLEALEELFDGITLASKAYNVDVIGGDTTSSQKGLIISITAIGEADENEIVY
                     RNGAKQGDLLVVTGDLGSAYMGLQVLEREKQVFQVNPNNQPDLDNYTYLIERQLKPEA
                     RTDIRELLEKLAVKPTAMIDISDGLSSEIIHICKKSNVGCNLYEEKLPVDPQLINVCE
                     EFNIDSTTVAINGGEDYELLFAIAIEDYDKIKANPNMTVIGHLTQESEGIHLITRANT
                     KIPLKARGWNALEAE"
     gene            2775..3650
                     /locus_tag="PK218_09150"
     CDS             2775..3650
                     /locus_tag="PK218_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW98716.1"
                     /translation="MKLQISNLSKTYPNGVKALDNLNLEIGTGMFGLLGPNGAGKSSL
                     MRTIATLQKPDSGSITFEGINILENQMELRKILGYLPQEFGVYPSMSAEKLLDYFARL
                     KGISSSKDRKIIIKEVLEVTNLYDVRMKNVSGYSGGMKQRFGIAQLLLNNPKLIIVDE
                     PTAGLDPAERHRFLNVLREIGTNHTVIFSTHIVDDVKELCTEVAILNGGKILNQNTPA
                     AAVEALENKIWTKVVSRDELEPSEKEFNVISSNYNQDNTLNIRVFSETQPNETFKKAS
                     PQLEDVYFVALKNDQ"
     gene            3652..7299
                     /locus_tag="PK218_09155"
     CDS             3652..7299
                     /locus_tag="PK218_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPW98717.1"
                     /translation="MFGTIFTFELKRWFRNPTLYIYVAIFFFLAFFIMASSLGVFDSF
                     KTTTSSRAISNSPIALNGIINGLTVFIYFLLPSIIGASVYRDFKYNMHSILFSYPFTK
                     WEYLFGKFLSSLLVVIFITLFLGLAAYLASFLPGVNQEMLGPQRIMAYLQTYLIFIIP
                     NLLFYGVIIFSVVTITRNISVGFITVILLLFIQSIVSSFTKDADNRYLVALFEPFGSD
                     ALDYYTKYWTMYEKNNNLLPFEGVIIYNRLLWFGVSVLIFGLIYRYFSFTQTALTLGR
                     SKNKERVVKNNFGGITKINLPQVSFDYSLKQNLKTTWSLSNLDFKFIVKNWVFISILI
                     VGLLFILITASVIGEVFGTNTYPVTWQMLLIPGGTFGLFINLVTFLFAGLLIHRGTTT
                     RMNHLIDSTPVPNWVLLTSKFIALIKMQLILLFVILLAGVLYQTYSGFYDYEIGHYLK
                     ELLGLKLIHFVIWAFLAIIIQTLFKNYLLGFFVLLILSIGLNFLPAIGVEQDIFIYNS
                     DSGFQYSDMNGYGHGLNLYLIYKIYWFLLGITFFCLALLFWTRGIGQSAKERFKEAGR
                     RLHSKIAISLTLSFVGFLAIGSTIYYQNNVINKTKSGKESEKEMVQWEKKYKKYEHKP
                     QPRITDIKMDMDLYPKERNFVANGTYILKNKTKVAIDTIYINHGKNTEVSFNVKNKLI
                     SKDTVFNFDIYRLEKSLIVGDSIQMKFVVKNKPNTFFRDNSPVEYNGTFINNSMFPSI
                     GYSDQSELTDDEVRKKYGLKPRDRMPKPTDSIARQNTYISNDADWVTFETTVSTSNDQ
                     TAIAPGYLIKKWTKDGRNYFHYKMDRKMLNFYAFNSARYEVKKDKWHDVNIEIYYNKG
                     HEYNIDRMISGVKKSLDYYTKNFSPYQHKQVRIIEFPRTGGGFAQSFANTIPFSEAIG
                     FIAKVDDEDQNGVDYPFSITSHEVAHQWWAHQVIGANVQGATLLSESLSEYSSLKVLE
                     HTYGKTQMRKFLKDALDSYLQGRTFENKKEQPLMYNENQQYIHYNKGSLVLYALSDYI
                     GEKNMNNALKRYIQKVAFQEAPYTNSIELVQELDAATPDSLKYLIHDMFETITLYENR
                     VEKATAKKLPNGTYEVKIDFTVSKYKANDDGKRLFKDKTGKTVTYKKKDSKYAIESYP
                     LNDYVEIGVFSEQTVKGKKKDKELYLKKVKIDKINNSVTVIVKEKPLEVGVDPYNKLI
                     DTQSDDNRIKI"
     gene            complement(7349..7828)
                     /gene="rnhA"
                     /locus_tag="PK218_09160"
     CDS             complement(7349..7828)
                     /gene="rnhA"
                     /locus_tag="PK218_09160"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HPW98718.1"
                     /translation="MQHQVHIYTDGAAKGNPGKGGYGVVMELVGTSYKKEFYEGFRLT
                     TNNRMELLAVIVGLEKITKPNMSVLVISDSKYVVDSVEKKWVFGWEKKNFSGKKNPDL
                     WIRFLKIYRKHQVDFKWIKGHNNHPQNERCDELAVYASGLPNLSIDAYYEKENDTLF"
     gene            complement(7838..8407)
                     /gene="purN"
                     /locus_tag="PK218_09165"
     CDS             complement(7838..8407)
                     /gene="purN"
                     /locus_tag="PK218_09165"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPW98719.1"
                     /translation="MKKIVLFASGNGSNVENIIHYFKNNSNVSVVGVFSNNQHAKVLE
                     RAKNHNIYSESFTNIELNDGTVFQKIAELNPDLIVLAGFLLKFPDTIINAFPNKVINI
                     HPALLPKYGGKGMYGNHVHQAILDNKEKETGITIHFVNEHYDEGEFIFQKELNIENCR
                     TSEEIATKVQELEHKHFPEIIEVLLTQNQ"
BASE COUNT         2986 a         1406 c         1456 g         2785 t
ORIGIN      
        1 caactcgaaa ccgacgaaat catccaagaa aaagaccagc ttgaagataa aaacagaaac
       61 ttactttatt tcttttttgg ttcggttggg ttgtttgggt ttttatatta cctcagaacc
      121 caacgtgcta aaaaccgaga gttgatttat aaacaggcac aacaaagagc caatgaagaa
      181 atctttaatc tcatgatgtc gcaacaagcc attattgacg aaagccgaac gcaagaaaaa
      241 caaaaattag cacgcgatct tcacgatggt gttttgggca gaatgtttgg acttcgcttg
      301 agtttagaca gtgtaaaccg aaagaatgat gaagcttcct tgttaaaacg acttcaaatt
      361 attgacgagc taaaaaccat agaacaagat attcgcgaaa tttctcatga cctaaatcga
      421 gaaaaacaag ttttaattaa taactttgtg tccattgtaa acaatttgat tgaagaacaa
      481 aaagaaaaac accatgctaa agtttcgttc tttttagaca cttccattca ttgggacaaa
      541 atagcaagtg ctgtaaaaat taatttatac aggattttac aagaaagttt gcaaaatatc
      601 aacaaatatg ccaacgcaaa aaacattaat attggttttg tcaaaaatga agacaccatt
      661 atttttaaaa ttagtgatga cggaattggt tttgacgcca acaaaaaaac aaaaggaatt
      721 ggaactcaaa atatggtagc tcgaacaaaa gaatgcaaag gtacaattga tattcaatct
      781 gaaataggag aaggaacaac cattacgatt acaattccaa cagaaaacaa ataacaaaat
      841 aatttacctg actgaatgga aaacccaatt aacgtattaa tagtagatga tcatccattt
      901 atcattgaag cttataaaaa cgccttgaat aaatacagtc ttcaaggcta tgaatttaac
      961 gttacacaag cagccgactg caaatcgggt tatgaattaa ttgtttcgga tgataataat
     1021 tataatctag ccatgtttga tgttagtatg cccgaatatg ttgagaaaaa cattcgctcc
     1081 ggaatggatt tggcaaaatt aatgcaaaac gaaaacccaa gctgcaaagt catgctacta
     1141 actatgcacg tagaattaga ccgaataaat gctattatca aagaaataaa tccaaatagt
     1201 ttattgatta aaaacgacat caattttgat gaattaattt ttgcgctaga taaaacgtta
     1261 aaaggcgaca aatattatag cgaaaccatc attaaattac taggtcaagg tgaatttatt
     1321 aaagtagaat tcgatgctat ggacaaaaga attttagagt tgatggccaa agaatacgaa
     1381 accatcttaa tggcaagaca tttaccgcta acaccagttg ttatagaagt tcgaaagaaa
     1441 agaattagag aagtgtttaa tcttcctgat gctaccgatg cagaaattgt tgaacaagct
     1501 agagcaaaac aaatcattga ataaaaaaac gtccatttgg acgtttttta tttttattca
     1561 gcttctaaag cattccaacc gcgtgctttc aatggaattt tagtgtttgc tctagtaatg
     1621 agatgaatcc cttcgctttc ttgtgttaga tgaccaatta ctgtcatatt tggatttgct
     1681 tttattttat cataatcttc aatggcaatt gcaaataata attcataatc ttctccaccg
     1741 tttatggcaa cagtagtgct atcaatatta aactcttcgc aaacattaat taattgtgga
     1801 tcaaccggca gtttttcttc gtataaattg catccaacgt tacttttttt gcaaatatga
     1861 atgatttcgg aagacaaacc atccgaaata tcaatcatcg ctgttggttt tacagctagt
     1921 ttctctaata attctctaat atccgttcgt gcttcaggtt ttagctgacg ttcaatcaaa
     1981 taggtgtaat tatccaaatc gggctggttg ttcggattga cttgaaaaac ttgtttttca
     2041 cgttccaaaa cttgcaaacc catataagcc gaacctaaat cgccagtaac tactaacaaa
     2101 tcgccttgtt tagcaccatt tctatagaca atctcatttt catctgcttc gccaatggca
     2161 gtaatggaga taatcaatcc tttttgagaa gaagtggtgt cgccaccaat aacatcaaca
     2221 ttataagctt ttgaagccaa cgtaatgccg tcaaataatt cttccagcgc ttctaaagga
     2281 aaacgattgg aaacggcaac agaaaccgtt atttgcgttg gtttggcatt catcgcagca
     2341 atatccgaaa gattaaccac aaccgcttta taacccagat ggcgtaatgg catataggct
     2401 aaatcaaaat ggacaccttc aatcaataaa tcggtagaaa caaccacttt tttggcggca
     2461 aaatctaaaa ccgcggcatc atcgccaata ctttttatgg ttgaaggttg ttttacatca
     2521 aaatctttgg tcaaatggtc aattagtcca aattcaccca attgagataa ggacgttttt
     2581 tgtgggtttt tatcttctag catggattgt ttttcaaaaa ttaatggcgc aaagttacaa
     2641 attgaagggt gtttttccac atgaatacaa ctttcgtttt caaataacgt aaaagtgtta
     2701 aaatttttgg taatctaatg taacacaatg ttaaaaacca atacctatac ataaacgata
     2761 atttttccct ttttatgaaa ctacaaataa gcaatttaag caaaacttat ccaaatggtg
     2821 tcaaagcttt ggacaacttg aatttagaaa tcggcacagg aatgtttgga ttgcttggtc
     2881 caaatggagc tggaaaatct tctttaatgc gaactattgc tacgctccaa aaaccagatt
     2941 cgggttcgat tacttttgag ggtatcaata tattggaaaa tcaaatggag ttgcgaaaaa
     3001 ttttgggtta tttgccacaa gaatttggtg tttatcccag tatgtctgcc gaaaaattat
     3061 tggattattt tgctagatta aaaggcatct cttcgtcaaa agatagaaaa ataatcatca
     3121 aagaagtgct ggaagtgacc aatctatatg atgttcgtat gaaaaacgtt agtggttatt
     3181 caggtggaat gaagcaacgt ttcggaattg cacaattatt attaaacaat ccaaaattaa
     3241 ttatcgttga tgaaccaaca gcaggacttg acccagccga aagacatcgt tttttgaatg
     3301 ttttaagaga aattggcact aatcataccg tgattttttc aacacacata gtagatgatg
     3361 taaaagaact atgtactgaa gtagccattt taaatggtgg aaaaatttta aatcaaaaca
     3421 caccagcagc agcagttgaa gcacttgaaa ataaaatttg gactaaagtt gtttctcgtg
     3481 atgaactaga accatctgaa aaagaattca atgtgatttc gtccaattac aaccaagaca
     3541 atacgctaaa tattagagtt tttagcgaaa cacaacctaa tgaaaccttc aaaaaagcat
     3601 cgccccaact agaagatgtt tattttgtag cattaaaaaa cgaccaataa tatgtttgga
     3661 acaatattta ccttcgaatt aaaaagatgg tttagaaatc caaccttata tatttatgtt
     3721 gcgattttct ttttcctagc tttttttata atggcaagca gtttgggcgt ttttgattcc
     3781 ttcaaaacga caacttcttc tagagcaatt tcaaattcac cgatagcttt aaacggaatt
     3841 atcaatggat taacggtttt catctacttc ttgttgcctt ccattattgg tgcttctgtg
     3901 tatcgcgatt ttaaatacaa tatgcactct attctctttt cctatccatt tacaaaatgg
     3961 gaatatttgt ttggaaaatt tcttagttcg ctattggtgg ttattttcat aacactattt
     4021 cttggtttag ctgcctattt ggcttctttt ttacctggtg ttaaccaaga aatgcttggg
     4081 ccgcaaagaa taatggccta cttgcaaact tatttgattt ttatcattcc aaatttactt
     4141 ttttatggtg taattatttt ttcggtggta accattactc gaaacatttc ggttggtttt
     4201 attaccgtga ttttactatt attcatccaa tccattgtca gtagctttac taaagatgct
     4261 gataatcgtt atttggtagc gctttttgaa ccgtttggtt ccgatgcatt ggattattat
     4321 accaaatatt ggacgatgta tgaaaaaaac aacaatctgt tgccttttga aggcgtgatt
     4381 atttacaaca gattgttatg gtttggtgtt tcagtattga tttttggatt aatttatcgt
     4441 tatttttcgt ttacacaaac ggcgttaacc ttgggaagaa gtaaaaacaa agaacgagtt
     4501 gtcaaaaata attttggtgg catcactaaa atcaatttac cccaagtttc ttttgattat
     4561 tctctaaaac aaaacttaaa aaccacatgg agtttgtcta atttagattt taaattcatt
     4621 gtaaaaaatt gggttttcat cagtatttta attgttggtt tattatttat attgataact
     4681 gcttcggtta ttggagaagt ttttggaacc aatacttatc ctgtaacttg gcaaatgctt
     4741 ctaattccag gcggaacttt tgggctattt atcaatttgg tgactttcct ttttgcgggt
     4801 ttactaattc atcgtgggac gactaccaga atgaaccatt taattgattc tacaccagtg
     4861 ccgaattggg ttttgttaac ttctaaattt attgcgctga taaaaatgca attgatttta
     4921 ttgtttgtaa ttcttcttgc tggtgtttta taccaaacgt attctgggtt ttatgattac
     4981 gaaattggtc attatctaaa agaattatta ggactgaagc taattcattt tgtcatttgg
     5041 gcgtttttgg ccatcattat tcaaacttta tttaagaact acctacttgg attttttgtg
     5101 ttgcttatct tatccatagg attgaatttc cttccagcta ttggtgttga gcaagatatt
     5161 tttatttaca acagcgattc tggttttcaa tattccgata tgaatgggta tggtcatggg
     5221 ttgaatttgt atttaattta taaaatctat tggtttttgc tgggtattac ttttttctgc
     5281 ttggcattat tgttttggac acgaggcatc ggtcaatcgg caaaagaacg ttttaaagaa
     5341 gctggaagac gattgcattc taaaatagcc atatcgctaa cactttcttt tgttgggttt
     5401 cttgccattg ggagcaccat ttattaccaa aataatgtaa tcaacaaaac caaatcgggt
     5461 aaagaatctg aaaaagaaat ggtacagtgg gaaaaaaaat ataaaaaata cgagcacaaa
     5521 ccgcaacctc gaattacaga cataaaaatg gacatggatt tgtatcctaa agagcgcaat
     5581 tttgttgcca atggaacgta tatcttaaaa aacaaaacca aagttgctat agacactatt
     5641 tacatcaatc acggaaaaaa cacagaagtt tcattcaatg taaaaaacaa gttgatttcg
     5701 aaagatactg tgttcaattt cgatatttat cgtttggaaa aatctttaat tgttggcgat
     5761 tctattcaaa tgaaattcgt tgtaaaaaac aagccaaata ctttttttag agataattcg
     5821 ccagtggaat acaacggaac attcatcaat aactcgatgt ttcctagcat tggctattcc
     5881 gaccaaagtg aattgactga tgatgaagtg cgtaaaaaat atggtttaaa gccaagagac
     5941 agaatgccaa aacccacaga ttctattgct aggcaaaaca catatatcag taatgatgct
     6001 gattgggtaa cttttgaaac aaccgttagc acctctaacg atcaaacagc cattgctcca
     6061 ggttatttaa tcaaaaaatg gacaaaagac ggtagaaatt attttcatta caaaatggat
     6121 agaaaaatgt tgaatttcta tgcgttcaat tcggcacgat atgaagtcaa aaaagacaaa
     6181 tggcacgatg taaacatcga aatatattat aataaaggtc atgaatataa tattgaccga
     6241 atgatttcgg gtgtaaaaaa atcgttagat tattatacta aaaattttag cccatatcag
     6301 cataaacaag ttagaattat agaatttcca agaactggcg gtggatttgc acaatcgttt
     6361 gccaatacca ttcctttttc ggaagccatc ggatttattg ccaaggtaga tgacgaagac
     6421 caaaatggcg tagattatcc tttttcgata acttcgcatg aagtggcgca tcaatggtgg
     6481 gcacatcaag tcattggtgc caatgtgcaa ggagcaacct tgctttctga gagtttatcc
     6541 gaatacagtt cgctcaaagt gttagaacat acttatggca aaacacaaat gcgaaaattc
     6601 ttaaaagatg cgcttgatag ctatttacaa ggcagaacat ttgaaaacaa aaaagaacaa
     6661 ccgctgatgt ataatgaaaa tcagcagtat attcattaca acaaaggttc gctggtattg
     6721 tatgctttga gcgattatat aggtgaaaaa aacatgaata atgccttgaa acgatatatt
     6781 caaaaagtag cttttcaaga agcaccttat accaactcga tagaattagt acaagaactt
     6841 gatgctgcaa caccagacag tttaaaatat ctcattcatg acatgtttga gaccataacc
     6901 ttgtatgaaa atcgagtaga aaaagcgacc gcaaagaaat tgccaaacgg aacgtatgaa
     6961 gtaaaaatag atttcacggt ttctaaatac aaagcaaatg atgatggaaa gcgcttattc
     7021 aaagacaaaa caggaaaaac agtgacctat aaaaagaaag acagcaagta tgccatcgaa
     7081 tcgtatccgc taaatgacta tgtagaaatt ggtgttttct cagaacaaac cgtaaaagga
     7141 aagaaaaaag acaaagaatt gtatttgaaa aaagtaaaaa ttgataaaat caataattca
     7201 gttacggtaa ttgtaaaaga aaaaccgctt gaagttggcg ttgatccata taataaattg
     7261 attgataccc aatcggatga taatcgcatc aagatttaaa aaagtaagca aagccaagaa
     7321 gttatttttg gctttgtttt ttctaatttt aaaacaacgt gtcattttcc ttttcataat
     7381 aagcatctat agaaagatta ggtaaacccg aagcataaac agcaagttca tcacaacgtt
     7441 cgttttgagg atggttatta tgacctttta tccatttgaa atctacttga tgtttgcgat
     7501 atatttttag aaaacgtatc cacaaatctg gatttttttt acccgaaaaa tttttctttt
     7561 cccatccaaa aacccatttt ttctcaacag aatcgacaac atatttagaa tccgaaataa
     7621 ctagaacgct catgttgggt ttagtaattt tttccaaacc aacaattacg gctaacaatt
     7681 ccattcggtt gttggttgtt aatcgaaaac cttcgtaaaa ttctttttta taactagtac
     7741 ctaccaattc catcaccaca ccgtaaccac ctttgccagg atttcctttg gcagcgccgt
     7801 cggtatagat gtgaacttga tgttgcatga ttgtgaatta ctggttttgt gtcaaaagaa
     7861 cttctataat ttcagggaaa tgtttgtgtt ctaactcttg aacttttgtt gcaatttctt
     7921 ctgaagttct acaattctct atgtttagtt ctttttgaaa aataaattca ccttcgtcat
     7981 aatgttcatt tacaaaatga atagtgatgc cagtttcttt ctccttatta tctaaaatag
     8041 cttgatgaac atgattgcca tacattcctt ttccgccata ttttggcaat aaagcaggat
     8101 gaatattgat aactttattg ggaaaagcgt tgattatagt gtcggggaat tttagtaaaa
     8161 aaccagccaa cacaattaaa tcaggattga gttctgctat tttttgaaaa acagtaccat
     8221 cattaagttc tatattggtg aagctttccg aataaatgtt gtgatttttt gctctctcaa
     8281 gcactttggc atgctgattg ttagagaata caccaactac agaaacgtta gaattgtttt
     8341 tgaaataatg aatgatattc tcaacattgg aaccatttcc agaagcaaac aacactattt
     8401 ttttcatggt taaaaggttt gttcctgcaa aaaaaagaat atttcataga ttttaaatca
     8461 ttttagccta agaatttgaa attatttttt tactttcgta gttatattta aaaacaaaaa
     8521 aggttgtgtt aaaataaagt tttttatttt tgccaacaat taaaattaaa aattaaaaat
     8581 tatgtcagac attgcatcaa gagttaaagc gattatcgta gacaaattag gcg
//
LOCUS       DAOOBH010000096         8565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_10131_length_8565_cov_4.831022, whole genome shotgun sequence.
ACCESSION   DAOOBH010000096 DAOOBH010000000
VERSION     DAOOBH010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8565
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10131_length_8565_cov_4.831022"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PK218_09170"
     CDS             complement(<1..573)
                     /locus_tag="PK218_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPW98720.1"
                     /translation="MKKSVFDLDHQNASVESRIVASLERVSQAFRVLLWNESKEFSLS
                     PIQIQLLIFLLHHSDEKRKVSYLADEFNMTKATISDTVKTLVQKQLISKEYEPHDTRS
                     YIIHLTDKGKDVAEKTSLFAKQIQVPIDKLHETDKENLLLSLLEIIHHLNKAGIITIQ
                     RMCFTCHFYKSNENGQAHFCSLMNTKLADTE"
     gene            complement(570..944)
                     /locus_tag="PK218_09175"
     CDS             complement(570..944)
                     /locus_tag="PK218_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HPW98721.1"
                     /translation="MKKDIEDKNDIVLLVNSFYNAVQENEILGFIFNDVAKINWDEHL
                     PRMYAFWTSMLLNEHLFSGNPMEKHITLSKITSMTEIQFNEWLLLFTTTVDKLFKGEI
                     ANEAKFRAENIARLMLLKIQSV"
     gene            complement(944..1207)
                     /locus_tag="PK218_09180"
     CDS             complement(944..1207)
                     /locus_tag="PK218_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HPW98722.1"
                     /translation="MKVAVWDTYVTKKDTTVMHFDILVPEEIKDATQIYQYGKEYLKT
                     KNQEEQTLTSKECRFCHLETVKPHWEEAINEKGYFIIEMENCN"
     gene            complement(1288..1521)
                     /locus_tag="PK218_09185"
     CDS             complement(1288..1521)
                     /locus_tag="PK218_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPW98723.1"
                     /translation="MSKSIFYHAGCSVCVSAEHDIINLLGKDKVEVVHFGNDKSRIAE
                     AEKAGVKSVPALVTPNGNVLHINFGASMADVKG"
     gene            complement(1724..2026)
                     /locus_tag="PK218_09190"
     CDS             complement(1724..2026)
                     /locus_tag="PK218_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HPW98724.1"
                     /translation="MGKYNTLSEAINDLTKIGYTFNFNIKDDCVVCAENNSQLQPDEF
                     EIDQVHRFQEMSDVDNESILYAISSTQHNLKGLLVNAYGMYADAASAKLIAKLNVR"
     gene            complement(2179..2646)
                     /locus_tag="PK218_09195"
     CDS             complement(2179..2646)
                     /locus_tag="PK218_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HPW98725.1"
                     /translation="MKNLIGIDKNKAKKLTEILNKLLADYEIFYQNLRGFHWNIKGRE
                     FFELHVKFEELYNDAFIKIDEIAERILTLEGIPMHTYTDYLKTSSIKEENNITNGVDG
                     VKIIVKNFASIITTEREILELASEANDEGTVSLMSDYITQTEKTLWMLNSYLS"
     gene            complement(2679..3602)
                     /locus_tag="PK218_09200"
     CDS             complement(2679..3602)
                     /locus_tag="PK218_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HPW98726.1"
                     /translation="MNTETSNTNPLLCDIETGMCEMTSNETEVLITKTVQSNDKLIRV
                     VYFTDPICSSCWGIEPQLRKLKLEYGQFIEIEYRMGGLLPDWSYNSGGISKPVDVAHH
                     WDEVSIHYDMPIDGDLWLEDPLNSSYPPSIAYKAALLQDEIKANLFMREIREMVFLQK
                     KNITKWEHLATAAKNVGLNVAQLKTDFEGNAKELFNEDLILAKDYGVRGFPTIFFIDN
                     KGKKETIYGSKPYAFYETAILNIQPTIVKSEYSKNWETLFSKYNSLTAKEFSVLSGTP
                     RIESEIVLNELSDAGKLEKITTKNGSMWKRK"
     gene            complement(4455..4805)
                     /locus_tag="PK218_09205"
     CDS             complement(4455..4805)
                     /locus_tag="PK218_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPW98727.1"
                     /translation="MKIDKTKFAENWIASWNSHKLEDILSHYSDDIEITTPMIKMALG
                     IDNGSLKGKKEVADYWQKALVKLPDLHFELIDVTESVNSVALYYKSVMNKMAIEVMFF
                     NEHGKVNKMYAHYS"
     gene            complement(4950..5126)
                     /locus_tag="PK218_09210"
     CDS             complement(4950..5126)
                     /locus_tag="PK218_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98728.1"
                     /translation="MDTCELAKKKYGYKFGNAISSHIIGKYPHEQPDDPSGLCFDVHP
                     DNHQDILQLDKIGN"
     gene            complement(5545..5712)
                     /locus_tag="PK218_09215"
                     /pseudo
     CDS             complement(5545..5712)
                     /locus_tag="PK218_09215"
                     /inference="COORDINATES: protein motif:HMM:NF036423.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(6237..6440)
                     /locus_tag="PK218_09220"
     CDS             complement(6237..6440)
                     /locus_tag="PK218_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98729.1"
                     /translation="MKLNNQRVCIYPKDIQRITGKSYRQSTRLMQKIKKDLNKLENEF
                     LTIEEFCTYSGIKYEQVTHLIFG"
     gene            6707..7465
                     /locus_tag="PK218_09225"
     CDS             6707..7465
                     /locus_tag="PK218_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98730.1"
                     /translation="MARQKGIIKLKGTIGDITFYKTQDGHLAREKGGIDANRIASDPA
                     FQRTRENGSEFGRAGKAGKVLRTALRALLLNSADGRMVSRLTQTMVKVIQADMVSERG
                     LRNVIDGEVDLLVGFEFNIRGKLGTSLFAPFVANIDRVAGEINVDLASFIPANMIAAP
                     NGTTHFKIISAGAEIDFEAETFIETHSETAILPWNSVATAAISQVNAVTPASTKPLFL
                     ALGVEFYQEVNGAMYPLKNGAFNPLAVVKVDGGV"
     gene            7465..7860
                     /locus_tag="PK218_09230"
     CDS             7465..7860
                     /locus_tag="PK218_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HPW98731.1"
                     /translation="MVFVLTRTYFPDGTNGKLECEGKSICKTIELPWKMNETKVSCIP
                     EGKYFIRKRYSRKFQWHIEVIDVKDRGLILFHPANNALKELNGCIAPVTKISGPGLGL
                     MSRKAFVKLKSIAYKALDKKESVELIIQS"
     gene            7871..8086
                     /locus_tag="PK218_09235"
     CDS             7871..8086
                     /locus_tag="PK218_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98732.1"
                     /translation="MKRIINRAKAPTPKFFKVLRNVGLALAAVGGTILAAPIALPVIV
                     TTIGGYVAVAGGVLSAASQLTTTDDSQ"
     gene            complement(8320..>8565)
                     /locus_tag="PK218_09240"
     CDS             complement(8320..>8565)
                     /locus_tag="PK218_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98733.1"
                     /translation="ALWKTKFWHQFLKGSVIPFLSLQDIRKEFSMKVNQEIKDHEEHV
                     KSVQALKLLEQSEKRFHENLNLINDMRRVILYRYISK"
BASE COUNT         2633 a         1298 c         1286 g         3348 t
ORIGIN      
        1 ttctgtgtct gccagttttg tattcatcaa actacaaaaa tgtgcttggc cattttcgtt
       61 tgatttgtaa aaatgacaag taaaacacat tctctgaatg gttataattc cagctttatt
      121 caaatgatgg ataatttcta acaaacttaa aagcaagttt tctttatcag tttcatgtag
      181 tttatcaatc ggaacttgaa tttgtttggc aaataaagat gttttttcag caacgtcttt
      241 tcctttatct gttagatgaa taatgtagct tcttgtatcg tgaggttcat attctttaga
      301 aattagctgt ttttgtacaa gtgtttttac cgtatcactt atggttgctt tggtcatgtt
      361 aaattcatca gccaaataac ttactttcct tttttcgtca gaatgatgta ataaaaaaat
      421 aagaagttga atttgaattg gacttaaaga aaattcttta ctttcgttcc aaagtaatac
      481 tctaaaagct tgagaaactc gttcaagtga agctacaatt ctactttcaa cacttgcatt
      541 ttgatggtct aaatcaaaca cagatttttt catacagatt gaattttgag taacattagt
      601 cgtgctatat tttcagctct aaattttgct tcattggcaa tttcaccttt aaataacttg
      661 tcaacagtag ttgtaaaaag caaaagccat tcgttaaatt gaatttctgt cattgaagtg
      721 attttgctta aagtgatatg tttttccatt ggattgcccg aaaaaagatg ctcatttaat
      781 aacatacttg tccaaaaggc atacattctt ggcaaatgtt cgtcccaatt tattttggca
      841 acatcattaa aaataaaacc taatatttca ttttcttgaa cagcgttgta aaaagaattt
      901 actagtaaaa caatatcatt tttgtcttca atgtcctttt tcattaattg caattttcca
      961 tttctatgat gaagtatcct ttttcgttga ttgcttcttc ccaatgaggt ttaacggttt
     1021 ctaaatgaca aaatctacat tcttttgagg ttagagtttg ttcttcttga tttttagttt
     1081 ttaaatactc ttttccatat tgatatattt gagttgcatc cttaatttct tcaggcacaa
     1141 gaatatcaaa atgcattact gtagtatctt ttttagttac ataggtgtcc caaacagcta
     1201 ctttcattct gttaatttta attgattatt tattgaatta aagatactcc acaaaggtag
     1261 ttatcctaac tttgtggatc aaatttttta cccttttaca tcagccattg aagcaccaaa
     1321 gttgatatgc aaaacatttc cgtttggtgt gaccaatgca ggaacagatt ttacacctgc
     1381 tttttcagct tctgcaattc tagatttgtc gttaccaaag tgaactactt ctactttgtc
     1441 ttttcctaaa agattaataa tgtcgtgttc agcacttaca catactgaac aaccagcgtg
     1501 atagaaaatt gatttactca ttttttttag atttaaatta tattgacact attgccaata
     1561 caaatatagt taggataact aacaatagca aataaaatta gtataacttt tttaaaatcg
     1621 tttacttatt tcaaaaatga attgtttgaa gcgatgatac tataaatagg tgaatgaatt
     1681 atttagtgct atgatctatt tcgaaaagct attagatata tatttatctg acgttcaatt
     1741 ttgcgattag tttagccgaa gcagcatcag cgtacattcc ataggcattt actaataatc
     1801 cttttaaatt atgttgtgtt gatgaaattg catataaaat actttcatta tcaacatcag
     1861 acatttcttg aaaacgatgt acttgatcaa tttcgaactc atccggttgt agttgcgaat
     1921 tgttttcggc acatactaca caatcatctt taatattaaa attaaaagta tatcctattt
     1981 tggtaagatc atttatagct tctgataaag tgttatattt tcccatttta ttatgtttta
     2041 ggatttagtt taaaattaat attcaataat aaattttttt gcagcgtata atagaatttt
     2101 aacgtattta aattcataac ataaaaatcc tttaatacgc tgtaataaag tataatagat
     2161 aaaagccaag atttacattt aactcaaata tgaattaagc atccaaaggg ttttctctgt
     2221 ttgagttata taatcactca ttaaacttac agttccttca tcatttgctt cacttgcaag
     2281 ttctaatatt tctctttctg tagtaataat agaggcaaaa tttttcacaa ttatcttaac
     2341 gccatccact ccattagtaa tattgttttc ttctttaatt gaagaagttt ttaggtaatc
     2401 ggtataagtg tgcataggaa ttccctctaa tgtaaggatt ctttcagcta tttcatctat
     2461 ttttataaag gcatcattgt ataattcttc aaatttaacg tgcaattcaa aaaattctct
     2521 tcctttaata ttccagtgaa atcctcttaa gttttggtaa aatatttcat aatctgctaa
     2581 tagcttattt aatatttcag taagtttttt tgctttgttt ttgtctattc ctattaaatt
     2641 tttcatgttt acctttttaa atttattaaa ttttagtttc attttcgttt ccacatagag
     2701 ccattctttg tggttatttt ttctaatttt ccagcatcgg aaagctcatt taaaacaatt
     2761 tcactttcta ttcttggagt acctgaaagc acagaaaatt cttttgcagt aagtgaattg
     2821 tattttgaaa aaagtgtttc ccaattttta ctgtattcgc ttttaacaat tgtaggctgt
     2881 atatttaata ttgcggtttc ataaaaagca taaggttttg aaccataaat ggtttctttt
     2941 ttacctttat tatctataaa aaaaattgtt ggaaatcctc ttacgccata atctttggct
     3001 aatatcaaat cttcattaaa taattctttt gcgtttcctt caaaatcagt tttcaattgt
     3061 gctacattca atcctacatt tttagcagca gttgctaaat gctcccattt agtaatattt
     3121 ttcttttgca aaaaaaccat ttctcttatt tctcgcataa ataagttggc ttttatttca
     3181 tcttgaagta gagctgcttt atatgctatt gatggtggat aagacgagtt gagaggatct
     3241 tctaaccaca aatcaccatc gataggcata tcgtaatgaa tacttacttc gtcccaatga
     3301 tgcgcaacat caactggttt actaattcca ccactattat agctccagtc tggcaacaaa
     3361 ccacccattc tatattcgat ttcaataaat tgtccatatt ctaacttgag ttttcttaac
     3421 tgtggctcaa ttccccaaca agaagaacaa attggatctg taaagtaaac tactcgtatt
     3481 agcttatcgt tagattgaac agttttggtt attaatactt ctgtttcatt gcttgtcatt
     3541 tcgcacattc cagtttcaat gtcgcataaa agcggattcg tatttgatgt ttcggtattc
     3601 atttttttgg tttttagttg tgcttgtgaa gctataaagc tcaacaataa aattagtaat
     3661 gcaaagaaat ttagttttat attcataatg caatttaatt tgcttcttct gaagttgcaa
     3721 actcctccat ttcgtaatca attgattatt gatggtatta acctgaaccc atttttgctt
     3781 taactctctg ccagaatgtt gtattttttg gtgttcttag cctaaaacca caactgtatt
     3841 tccttctaca ttggattatt gtgattcaaa gttgataatt tcagtttttt tcatattgtt
     3901 attaaaaaaa gttcttacac cttctttttt ttttcgaatc ttccagccga aattattttc
     3961 gtagcatgat aaatttatag tgtatttttg gaaactgttt caatgaattt ttctacatca
     4021 ccattactaa atgctgcgaa catttttcct actacttttt ttgcttaacc tttcattttt
     4081 acatatttat agttcacaaa tataacatct tacttatgtt tttctaaaaa aaattatatc
     4141 aataaagata atcaattcat tttattttaa ctactattta tctttagttt ttactttttt
     4201 aacaaacatt aacgtattca aattttctaa ctaaggaaaa actttgtcaa ttttagtttt
     4261 aattttttct aactcaacta atttcaaaat tttattaagc attttgatgt tttttttgag
     4321 atttatcagt agctagtatt acgtttattt tataaaaaaa agaggaactt agctttaatt
     4381 ttcctctttc tattaacttt aatagtatta gattgctttt gcaattgctt tttcagctat
     4441 tgaatttcta tttactatga atagtgcgca tacattttat ttactttccc atgttcatta
     4501 aaaaacatta cttcaattgc cattttgttc attaccgatt tatagtacaa agcaactgaa
     4561 tttacgcttt cagtaacatc aattaattca aaatgtaaat cgggtaattt tactaatgct
     4621 ttctgccagt aatcagcaac ttcttttttt ccttttagag aaccattatc aataccaagt
     4681 gccattttta tcattggagt agtaatttca atatcatcgg aatagtgtga aagtatatcc
     4741 tctaatttat gagaattcca agatgcaatc caattttctg cgaattttgt tttatctatt
     4801 ttcatgttta attttttttt ttcattaaaa tgttactgta ttaaaataaa tgggttagat
     4861 gacttttatt cattcgtcaa taattgctca aaaattacac caatattatt ttttctatct
     4921 acaaaatgta attcctacat ccgatgtctt tagtttccga ttttgtctaa ttgaagaata
     4981 tcttgatgat tgtctggatg tacatcaaag cataaaccag atgggtcgtc tggttgttcg
     5041 tgtggatatt taccaatgat atgacttgaa atggcatttc caaatttgta accatatttt
     5101 ttttttgcaa gttcacaggt gtccatttat tgaaaatgaa aaaaatagta gtatgtaaat
     5161 aatttttttc tcatcttttt tattttagaa ataataacat ctttagattc attaaagtca
     5221 ttgtagttat ttaaattaag attagtcata tattttaaaa aacacctaat ttgaaacggt
     5281 aggatttatt agtaaaaata caatgttttt tataaataat attaattaaa taatcccatt
     5341 aattttaaac tagttgcgtt taattctgaa tactatattt tattaaacgt atcttttaaa
     5401 actttcatgt catcactaac ttttctatcc aataattttg cataatgttg cgtagttctt
     5461 aaatttttgt gtccaagtat tttgcttaca ctttctattg gtacaccatt ggtatgagat
     5521 tgaatgtgct tcttccgaag tacttttcat tttagttaac tcgctagacc attttaatct
     5581 ttctacaaac tttttggtac taaattcaga acgtttcccg tcaactgtta agcgcacata
     5641 aattgggatt tgtcctgccg cattggcttt tgtgcttttg gcataaaagt gaattgtgat
     5701 ttttgttttc attgtggaga ccaattaatt attactcaat ttaaattcct aattgaaatc
     5761 aaacaagatg tttgatgagt gaacaagcca ttgaacacgt ctttaaactg ccaatttgca
     5821 atgctttcga gaatttagcg agaccctaat ttttgaattt tctagggtct cgattttatc
     5881 tcgatttaaa taagattgaa taatttggta tgtctgcaaa agcaaaaacc ccgtaaatca
     5941 taagatttac agggttttga taccgtttag gattttttca gcggagaaag agggatttga
     6001 ctacctattt caacgtattc tataacagct cgttacaatt gaatttttat ttgggtaacc
     6061 gattttaccc cctttttaat cgtaaataat tcatttcatt tgtgagttgt aaatatactc
     6121 atttgcaatg atatagcaaa ttaaaattca ataaacatta ctaaaaattg aacaagttgt
     6181 tcaagtgttt gattgtctaa atcttgatta aaatttgttt ttgtatttct taaattttaa
     6241 ccaaaaatca agtgagttac ttgttcatat tttatgccac tatatgtaca gaactcttca
     6301 atcgttagga actcattttc gagcttgttg aggtcttttt ttatcttttg cattaatcta
     6361 gtactttgac gataactctt gcctgttatt cgttgtatgt ccttcggata gatgcatacc
     6421 ctctgattat tcaatttcat actttatcta tacctttgat atagctatgc tatacttctg
     6481 acatgcagaa aatcttttgc aatttagtta taatttttca tacttattta ttgtgttttt
     6541 atgttcattt aggacacttg tgtcgatttt ggacatatgg tacacttgtt actttttatt
     6601 tagtaaaatg ctttgtaaac tttgctttaa tcattcgaaa tattgttgca actgattgaa
     6661 agaatgaagg agattttaga tgaatgttta acaaattttt gaagttatgg ctagacagaa
     6721 aggcataatt aagttgaaag gtactatcgg agatattacc ttttacaaaa cgcaagatgg
     6781 gcacttagca agagaaaaag gcggaattga cgctaataga atcgcgagtg atcctgcgtt
     6841 tcagagaact cgtgaaaatg gttccgagtt tggtagagct gggaaagccg gaaaggtttt
     6901 aagaacagct ttgagagctt tgcttttgaa ttctgccgat gggagaatgg taagccgttt
     6961 gactcagact atggtgaaag ttatccaggc tgatatggtt agcgaaagag gtttaagaaa
     7021 tgttattgat ggtgaagtag atttacttgt tggctttgaa ttcaatattc gtggtaagtt
     7081 gggaacaagt ttgtttgctc cttttgttgc aaatattgat agagttgcag gtgaaattaa
     7141 cgttgatttg gcttcattta ttcctgcgaa tatgattgct gctccaaatg gaactactca
     7201 ttttaaaatc atttctgcag gagctgagat tgatttcgaa gcagagactt ttatcgaaac
     7261 tcactctgaa actgctattt taccttggaa ttcagttgct acggcagcta taagccaagt
     7321 aaatgcagtt accccagcaa gcacaaaacc actgtttttg gctttaggtg ttgagtttta
     7381 tcaagaagtt aatggtgcta tgtatccgtt gaaaaatggt gcttttaacc cattagcagt
     7441 ggtaaaagtt gatggtggtg tgtagtggtt tttgtactca ctagaactta tttccctgat
     7501 ggaactaatg gtaaactcga atgcgaaggc aaatcgattt gtaaaacaat tgaattgcca
     7561 tggaagatga acgaaacgaa ggtttcttgc attcctgaag ggaaatattt tattagaaag
     7621 cgatatagca gaaagtttca atggcatatt gaagtcattg atgtaaaaga tagaggttta
     7681 attctatttc atcctgctaa taatgctttg aaggaattga acgggtgtat tgcacctgta
     7741 acaaagatta gtggacctgg attaggtttg atgtcgagaa aagcatttgt gaagttaaaa
     7801 agtattgctt acaaagcttt agataaaaaa gaaagtgttg aattgattat ccaatcttga
     7861 atattagatt atgaaaagaa ttattaatag agcaaaagct cccacaccga aattttttaa
     7921 agtgcttcga aatgttggtt tggcattagc tgctgttgga ggaactattt tagctgctcc
     7981 aattgcttta ccagtcattg taacaaccat tggtggttat gttgcagtag caggaggagt
     8041 tttgtcagct gcaagtcagc ttactacaac tgatgacagc caataatccc acattgatgg
     8101 gaactgctgg aggcacattt ttaagtattg taccgaatct ttcctctaat gatattgcaa
     8161 aaactgttat attagctact attggagctg ttgtcagttt tacgatttcg ttactgctta
     8221 aaagcctaag taagaagcac aaaaaataag tttagctcta gttgtggtga cctttgggtt
     8281 aaacaaagtg aaagccaagt cgtgagacct ggcttttttt tatttactta tatatctgta
     8341 taaaattact cttcgcatat cattgataag atttagattt tcatggaaac gtttttcgct
     8401 ttgttccaaa agctttaatg cttgtacgct tttaacgtgt tcttcgtggt cttttatctc
     8461 ttgattaact ttcattgaaa attcttttct aatgtcttgt aagctcaaaa atggtattac
     8521 tgaaccttta aggaattgat gccaaaattt tgttttccaa agtgc
//
LOCUS       DAOOBH010000097         8559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_10140_length_8559_cov_5.987888, whole genome shotgun sequence.
ACCESSION   DAOOBH010000097 DAOOBH010000000
VERSION     DAOOBH010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10140_length_8559_cov_5.987888"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PK218_09245"
     CDS             <1..806
                     /locus_tag="PK218_09245"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HPW98734.1"
                     /translation="GKVFIYRNIANDDYINDLLSVNVELTTEEKENPSLHFAKLETFN
                     TQLLEIVYKHREEELETDGFEYVQKVSEIIKLYELNEDKIVESIDYIGTSKNIIPAFK
                     YIAEPLKLEFYLSLILALKYGKEFAIRPNYKADYIGLPISHAPGNTGDIEVYSKKLYW
                     LIEVTLIRNKTQQLNSETTSVIRHFLEDNKINNYLSKYLSFVAPFIHQDTREFFDYSI
                     VRHKIKDQRFNLKPYSISEFIDTTLTSENFKNMESYTSKVIEDFRNNLI"
     gene            complement(1103..1621)
                     /locus_tag="PK218_09250"
     CDS             complement(1103..1621)
                     /locus_tag="PK218_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPW98735.1"
                     /translation="MKPKDINPNEHSAYFANYINQVSDEYTLVEELEISVHRLIKFVQ
                     DIPMDKFDYRYAEGKWTIKDILQHIIDAERIFAYRALRIARNDKTELPGFDEDDYANN
                     ANGSSRSIQSLLTELAVVRQATLSLFKSLSDDELKRIGTASNNPASVRAIGFIIIGHQ
                     NHHQKVFEERYL"
     gene            complement(1634..4210)
                     /locus_tag="PK218_09255"
     CDS             complement(1634..4210)
                     /locus_tag="PK218_09255"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HPW98736.1"
                     /translation="MYTLPKMERFNQNVVSKYQVYNSVFMTLPFDAIDNTGVLLPLFA
                     EICDNGYKNNWSPEQIINFFSNKYLDNATEKDKVDLMFRFIQYIERQIVLFDAIEDAA
                     FSIINNLEGRGSLRDIKEKTEAKNKREELIQFLEEFKVRTVLTAHPTQFYPGAVLGII
                     TDLTEAIRQNDLVQIKQLLAQLGKTPFFKKEKPSPFDEAISLIWYLENVFYQTIGDMM
                     QYMQKNLYDGKIIENPIISLGFWPGGDRDGNPFVTTEITLKVAERLRTSILKCYYFDV
                     RKLRRKLTFTGVETIISELENKLYRSVFYSKGEIFITCDEFKSQLLKIKEILIHNHQS
                     LYVDYVDALIQKVISFGFHFASLDIRQNSRVHGKVVDDIVNYLIEQQTSGFPKNYNEL
                     NETAKIDALLLVRSTIAPENFEDQITKLTLESILAIKTIQENNGELGCNRYIISNNES
                     ALDVMETFSMFQLCGWENPTVDMVPLFESVDDLQKAHHILEQLYTNEVYKIHLERRKN
                     IQTVMLGFSDGTKDGGYLMANWSIFEAKETITAMSRKYGIKVIFFDGRGGPPARGGGK
                     THKFYASLGSTIENQEIQLTIQGQTISSNFGTLDSCRLNLENMLSAGVTNEIFNKDEN
                     NLTVSEKQTMQKLAELGYQKYIDFKNDPKFVPYLEQMSTLCFYAKTNIGSRPAKRNAD
                     DKLNFSDLRAIPFVGSWSQMKQNVPGFYGVGTALKYFEDNNRWEEVQLLYDNSLFFKT
                     LLENSMMSLAKSFFPLTSYMKKDKEFGAFWQIIYDEFLETKRLLLKIAGHKELMDNYP
                     DGKASIQIREQIVRPLLTIQQYALSQLRRGKIDDVELKEIYEKLVVRSLFGNTNASRN
                     SA"
     gene            complement(4288..4770)
                     /locus_tag="PK218_09260"
     CDS             complement(4288..4770)
                     /locus_tag="PK218_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPW98737.1"
                     /translation="MSKFRLDEIDLQILDMLIENTRIPFTDVAKKLLISAGTVHVRVK
                     KMEEQGIIMGSSLSLDYEKLGYSFIAYVGVFLNNTSQTKFVLERINDIPYVTVAHVTT
                     GKFNIFCKIRAKDTKHAKDVIFMIDDIEGVYRTETMISLEESINDKKRLMHTIFHELD
                     "
     gene            complement(4838..5983)
                     /locus_tag="PK218_09265"
     CDS             complement(4838..5983)
                     /locus_tag="PK218_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPW98738.1"
                     /translation="MRDYTNLFQENKEESISGRYIALQHIEPLLKKYNAKIIGSSVLD
                     KPIYKLEIGKGKTKILMWSQMHGNESTTTKALFDFINYLHSNSPESKALLEYFTFCLL
                     PMLNPDGAAAYTRENANEIDLNRDAKDLSQPESKVLRSVFDVFQPDYCYNLHDQRTIY
                     GAGTTGKPATVSFLAPAFDEDRNWNVNRKKAAAIIVAMNNELQKLIPGQVGRYDDSFN
                     INCVGDTFQFLNVPTVLFEAGHFPNDYEREITRKYIFIAILSGIQYVYENDIVCNEIE
                     DYLRISQNNVNFHDFVCKNVNIDYDSNKKCLNFAVHYKEVLQQDKIKFEAYISQIDNL
                     DDYFGHKEFDANGAAYFNEQNNFPIMDEKADFSIGNHIKFVNGILIC"
     gene            complement(5983..6534)
                     /locus_tag="PK218_09270"
     CDS             complement(5983..6534)
                     /locus_tag="PK218_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPW98739.1"
                     /translation="MRKLLYKFIFFKLLGWKISGTIDEKVTKCILMVMPHTSNFDFFI
                     GIFTRGIMNMEMNFVAKKELFVFPFGYYFKNVGGAALDRSGGKNLVDTIVDIFNSRKV
                     FRLAIAPEGTRKKVTELKTGFYYIALKANVPIIPVAFDYGKKEVKIGTPFHPTGNYDQ
                     DIKELAIFFKDVKGKNPEKQIEL"
     gene            6596..7258
                     /locus_tag="PK218_09275"
     CDS             6596..7258
                     /locus_tag="PK218_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPW98740.1"
                     /translation="MLQKLFSYLIPVNIYKKKSDVSQSLEVTWNNGQLVLDSKNTNYS
                     YGSLQRILRKGLKYIGFERIKRFENVLVLGVAGGSVIKTLVEEVKFKGKITGVEIDKD
                     IVAIAEKYFGLSKISNLEIIIEDAFEFVLRTKDKYDLIIIDIFQDTTMPNFLFQDFFI
                     NHINLLLKVNGFILFNTMVINEKDNQRNAEYKSKFNGNYSLRMYPKVEVHNELFTIKK
                     LS"
     gene            complement(7250..7978)
                     /gene="kdsB"
                     /locus_tag="PK218_09280"
     CDS             complement(7250..7978)
                     /gene="kdsB"
                     /locus_tag="PK218_09280"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPW98741.1"
                     /translation="MKIIAVIPARYASTRFPAKLLQDLGGKTVIQRTYEAAKNTQLFD
                     DVFVVTDSILIYDEIVSHGGKAIMSIKEHESGSDRIAEAVENLDVDVVVNVQGDEPFI
                     NDEPLAKVLDVFRTDFDKKVDLASLMFEINDEKEIENPNNVKVVTDQNGFALYFSRSV
                     IPFPREKNVGVRYMKHIGIYAFRKQALLDFYHLPMKSLEASEKLEQLRYLEFGKRIKM
                     VETHQGSIGIDTPEDLEKARKLLG"
     gene            8235..8486
                     /locus_tag="PK218_09285"
     CDS             8235..8486
                     /locus_tag="PK218_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98742.1"
                     /translation="MFYNKKNLSNYLGSFFLMLRTFCRMTEGSFKVLRAFCQLTEGSF
                     KVLRTFQTTWEHCFDIKNFLKDEPYMIGVIGILVLSAII"
BASE COUNT         2763 a         1469 c         1304 g         3023 t
ORIGIN      
        1 gaggtaaggt tttcatttat agaaacatcg caaatgatga ctacattaac gatttacttt
       61 cagtaaatgt tgaattgaca accgaagaaa aagaaaatcc aagtttgcat tttgcaaagt
      121 tggaaacttt caacacgcaa ttacttgaaa ttgtttacaa acatcgtgaa gaagaattag
      181 aaacagacgg atttgagtat gttcaaaaag tttcagaaat catcaaattg tatgaattaa
      241 acgaggacaa aattgttgaa agtattgatt atataggaac ttcaaaaaat attattcctg
      301 cgtttaagta tattgcagaa cctttgaaat tggaatttta tttgagttta attcttgctt
      361 tgaaatacgg aaaagaattt gcaattagac caaattacaa agccgattac atcggtttgc
      421 caatttctca cgcaccagga aacacaggag atattgaagt ttattcaaag aagttgtatt
      481 ggttaattga agttacacta attcgtaaca aaacgcaaca actgaacagt gaaacaacat
      541 cagtaattcg tcacttcttg gaagacaaca aaatcaataa ttatctttca aaatatcttt
      601 cgtttgttgc acctttcata catcaagata cgagagaatt ttttgattat tcaattgtta
      661 gacacaaaat taaagaccaa cggtttaacc taaaacctta ctcaatttct gaatttattg
      721 atacaacatt aacctctgaa aacttcaaga atatggaaag ttatacaagc aaagtaattg
      781 aagattttag aaataatttg atctaataat aacttgacta acggaatgtt ttatcctgaa
      841 ataggtttac acaaaagtgt aaaaatatgg aaatagattc cgctagaaat tatgcgggtt
      901 tagattggga gattgcggta gttttgttag atttgttttg ataatgattg tgtgcacgga
      961 ttgcaaatcc gagctatcgg gttaagattg atttttcttg aaggtttaac ggcgtttcaa
     1021 taaatgaaag caagccaaaa aaggaagccc taaagcttcc ttgtctatta ttttttttct
     1081 tcttaaaagt aaaagtttct ccttaaagat agcgttcttc aaaaactttc tgatgatgat
     1141 tttgatgtcc tataataata aaaccgatgg ctcttaccga tgctgggtta ttggatgctg
     1201 ttccaattcg ttttaattca tcgtctgaca acgatttgaa aagcgacaat gtagcctgac
     1261 gaacaacagc caattctgtc aacaaggatt ggatgctcct actcgacccg ttggcattat
     1321 ttgcgtaatc atcttcatca aaacctggta attcagtttt gtcatttcta gctatgcgca
     1381 aagcacgata ggcaaaaatt cgttcggcat caatgatgtg ttgcagtata tcttttatag
     1441 tccattttcc ttcggcatac cgataatcaa atttatccat cgggatatct tgcacaaatt
     1501 ttatcaagcg atgaaccgaa atttcaagtt cttctactaa tgtatattca tccgaaactt
     1561 gattaatata gttggcaaaa tatgctgaat gttcgtttgg gtttatgtct tttggtttca
     1621 tataatttat ttattaagct gaatttctac tggcattggt gtttccaaat aatgaacgaa
     1681 cgactagttt ttcataaatt tcttttaatt ctacatcatc aatcttgcct cttcgcaatt
     1741 gtgataatgc atattgttga atggttaaca acggtcgtac aatttgttct ctaatttgaa
     1801 tagaagcttt tccatcagga taattatcca tcaattcttt gtgtccagct attttcaaca
     1861 acaaacgttt ggtttctaaa aattcatcat aaataatttg ccaaaacgcg ccaaattctt
     1921 tgtctttttt catataagat gtcaatggga aaaatgattt tgccaacgac atcatgctgt
     1981 tttccaacaa ggttttaaag aatagagagt tgtcataaag caattgcact tcttcccaac
     2041 gattgttgtc ttcaaaatat tttaaagctg ttccaacacc ataaaatcct ggaacatttt
     2101 gtttcatttg actccaagaa ccaacaaacg gtatggctct caaatcggag aaattcaatt
     2161 tatcgtctgc atttcttttt gcaggacgac taccaatatt ggtttttgca tagaaacaca
     2221 gtgtactcat ttgctccaaa taaggcacaa acttgggatc gtttttaaaa tcaatgtatt
     2281 tttgataccc caattccgcc aatttttgca tggtttgctt ttcggatacc gtcaaattgt
     2341 tttcatcttt attaaaaatc tcattggtta caccagcact tagcatgttt tctaaattta
     2401 atctacatga atctaatgtt ccaaaatttg agctaatagt ttgcccttga atggttaatt
     2461 gaatttcttg gttttcaatg gttgaaccta acgatgcata aaatttatgt gtttttccac
     2521 caccacgagc tggcggtccg cctctaccgt caaagaaaat aactttaatt ccgtattttc
     2581 tagacatggc tgtaatggtt tctttggctt caaaaatact ccagtttgcc atcaaataac
     2641 caccgtcttt tgttccgtct gaaaaaccaa gcatcacggt ttgtatattt tttctgcgtt
     2701 ctaaatgaat tttataaact tcattagtat aaagttgttc caaaatatga tgtgcttttt
     2761 gcaaatcatc caccgattca aacaaaggca ccatgtcaac tgttggattt tcccaaccac
     2821 aaagctgaaa cattgagaat gtttccatca catccaaagc actttcgttg ttgctgatga
     2881 tgtatcgatt gcaacccaat tcgccattgt tttcctgaat ggtttttatt gctaaaattg
     2941 attcaagcgt taatttcgta atttgatctt caaaattttc tggagcaatc gtacttcgaa
     3001 ccaaaagcaa agcatcaatt tttgcagttt cgttcaattc gttataattt tttggaaaac
     3061 cactggtttg ctgttcaatt aaataattga caatatcatc aactactttt ccgtgtactc
     3121 tgctgttctg acgaatgtct aaagacgcga aatggaatcc aaaactaatc actttttgaa
     3181 tcaaagcatc tacataatct acatacaatg attggtgatt gtgaatcaat atttctttaa
     3241 ttttcaataa ttgcgacttg aattcatcac aagtaatgaa gatttctcct tttgagtaaa
     3301 aaacagaacg atagagtttg ttctctaatt ctgatataat ggtttcaaca cccgtaaacg
     3361 ttaattttct tctaagtttc cgaacatcaa aatagtaaca cttcagaata gaagttcgca
     3421 atctttcggc aactttcaag gtaatttcgg tagttacaaa agggtttcca tctctatcac
     3481 caccaggcca aaaccctaaa ctaatgattg ggttttcgat tatttttcca tcataaagat
     3541 ttttttgcat gtattgcatc atgtcgccaa tggtttgata aaaaacattt tcaagatacc
     3601 aaatcaaact tatggcttca tcaaaaggcg atggtttttc ttttttgaag aatggcgttt
     3661 ttcccaattg cgccaacaat tgttttatct gaaccaaatc attttgacga atagcttccg
     3721 ttaaatctgt aattattcca agtactgcac caggataaaa ttgggttgga tgtgctgtca
     3781 aaaccgttcg gactttaaat tcttctaaaa attgaattaa ttcctcacgc ttatttttag
     3841 cttctgtttt ttcttttata tcgcgaagcg aacctctacc ttctaagtta ttgataatcg
     3901 agaaagcagc atcttcaatg gcatcaaaca aaacgatttg tcgttcgata tattgtatga
     3961 aacgaaacat caaatctact ttgtcttttt cagtagcgtt gtctaaatat ttattggaaa
     4021 agaagtttat tatttgttcg ggcgaccaat tatttttata accgttatca caaatttcgg
     4081 caaataacgg caataaaaca ccagtgttat caattgcatc aaatggtaat gtcataaaga
     4141 cactattgta cacctgatat tttgatacta cgttttgatt gaaccgctcc atttttggta
     4201 aagtgtacat gctctttttt tgtgttaagt taataaaaaa acctcaagca caaaacttga
     4261 ggttagaata tttttgaata ctatttctta atctagttcg tggaaaatag tgtgcattaa
     4321 acgtttttta tcattaatgc tttcttccaa tgaaatcatt gtttctgttc tataaacacc
     4381 ttcaatgtca tcaatcataa agattacatc tttggcatgt ttagtgtctt tagctctaat
     4441 tttacagaaa atattaaact taccagttgt tacgtgtgct actgttacat aaggaatgtc
     4501 attaattcgc tctagaacaa atttagtttg cgaagtattg tttaagaaaa caccaacgta
     4561 agcaataaac gaataaccta atttttcata gtctaatgac aaagatgaac ccataattat
     4621 cccttgttct tccatctttt taacacgaac gtgaactgtt cctgccgata tcaataattt
     4681 tttagcaaca tctgtaaaag gaattcgggt attctcaatc aacatatcca atatctgaag
     4741 atcaatttca tctaatctaa atttactcat tttttctttt ttttttatca tgtatttttt
     4801 cgacctgcaa aataaagtat tcttaacatt aaaattttta acaaatcaat attccattaa
     4861 caaatttaat gtgatttcct attgagaaat ccgctttttc atccattatt gggaaattgt
     4921 tttgttcatt gaaataagct gctccatttg catcaaattc tttgtgtcca aaataatcat
     4981 caagattatc aatttgtgaa atatatgctt caaatttaat tttatcttgc tgaagaactt
     5041 ctttatagtg cactgcaaaa ttcaaacatt ttttattaga atcataatct atattgacat
     5101 ttttgcacac aaagtcgtga aaattaacat tattttgaga aattctcaaa taatcttcaa
     5161 tttcattaca aactatatcg ttttcgtaaa catattggat tcccgaaagt attgctataa
     5221 aaatatattt tcgagtaatt tctcgttcat aatcattagg aaaatgtcct gcttcaaaca
     5281 aaacggttgg tacatttaaa aactgaaacg tatcgccaac acaatttata ttaaaagaat
     5341 catcatagcg accaacttga ccaggaataa gtttttgcaa ttcgttattc atagctacaa
     5401 tgattgctgc agcttttttt ctgttcacat tccaatttct gtcttcatca aatgctggtg
     5461 ccaaaaaaga aactgttgct ggtttgccag ttgttcccgc accataaata gtgcgctgat
     5521 cgtgtaaatt atagcagtaa tcaggttgaa agacatcaaa gacactgcgc aaaactttac
     5581 tttctggttg ggataagtct tttgcatcgc gattcaaatc gatttcattg gcattctctc
     5641 tagtataagc tgctgcgcca tcaggattga gcattggcaa caaacaaaag gtaaagtatt
     5701 ctaataaagc tttgctttct ggcgaatttg aatgcagata gtttataaaa tcaaacaatg
     5761 ctttggtagt ggtgctttcg tttccgtgca tttgcgacca cattaatatc ttggtttttc
     5821 ctttacctat ttctaattta taaataggtt tgtcaagcac agaacttcct attattttgg
     5881 cattatattt ttttaataat ggctcaatgt gttgcaaagc aatgtatcta cccgaaatac
     5941 tttcttcttt attttcttga aataaatttg tataatctct cattataatt caatttgttt
     6001 ttctggattt ttccctttta catctttaaa aaatatagct aattccttta tatcttgatc
     6061 ataattaccc gttggatgaa atggtgttcc tattttcact tctttttttc catagtcaaa
     6121 tgccactgga attataggaa cattggcttt tagagcgatg tagtaaaatc ctgttttgag
     6181 ttctgttacc ttttttcgag taccttcggg tgctatggca agtctgaaca cttttcttga
     6241 attaaaaata tcgactatgg tatcgactaa attttttcca ccacttctat ctaaagcagc
     6301 accgccaaca tttttaaaat aataaccaaa tgggaataca aacaattctt ttttggccac
     6361 aaaattcatt tccatattca taattcctct agtaaaaatt ccaataaaaa aatcgaaatt
     6421 gcttgtgtga ggcatgacca tcaaaataca tttggtaact ttttcatcaa tagttccact
     6481 gattttccat cccaagagct taaaaaaaat aaatttataa agtagttttc gcatggttgt
     6541 tattttttgg taaatttagt atttatgtag tatatttgag aaaacaagtc tattaatgct
     6601 acaaaaacta ttcagttatc ttatacctgt aaacatctac aaaaaaaaat cagatgtaag
     6661 tcaatcactt gaagtgactt ggaataatgg tcaattggtg ttagatagta aaaatacaaa
     6721 ttattcttat ggcagtttgc agcgaattct tagaaaagga ctgaaataca ttggatttga
     6781 aagaataaaa agatttgaaa atgtgttagt gcttggtgtt gctggtggaa gtgttataaa
     6841 aactctggtg gaagaagtaa aattcaaagg caaaattact ggtgttgaaa tagacaaaga
     6901 cattgtagca atagctgaga aatactttgg attatccaaa atttctaatc ttgaaatcat
     6961 aattgaagat gcttttgaat ttgttttacg cactaaagac aagtacgatt taatcatcat
     7021 tgatattttt caagacacta ctatgcccaa ctttttattt caagattttt ttatcaatca
     7081 cattaactta ctacttaaag taaatggttt tatccttttt aacactatgg ttataaatga
     7141 aaaagataat caaagaaatg ctgaatacaa atcaaaattc aatggtaact attctttaag
     7201 aatgtatcca aaagttgaag tgcataatga gttgtttacc atcaaaaagt tatcctaata
     7261 actttcttgc tttctctaaa tcttctggcg tatcaatgcc aatgctgcct tgatgggttt
     7321 caaccatttt tatgcgtttg ccaaattcaa gatagcgcag ttgttctaac ttttccgagg
     7381 cttcaagcga tttcatgggc aaatgataaa aatccaacaa tgcttgtttt ctaaacgcat
     7441 aaattccgat gtgtttcata tagcgaacgc ccacattttt ctctcttgga aacggaataa
     7501 cggaacgcga aaagtataac gcaaagccat tttgatcggt tactactttt acattattgg
     7561 ggttttcgat ttctttttca tcattaattt caaacatcaa cgaagccaaa tctacttttt
     7621 tatcaaaatc agttctaaaa acatccaaaa cttttgccaa aggttcgtca ttaataaacg
     7681 gttcgtcgcc ttgcacatta accactacat caacgtctaa attctcaact gcttcggcta
     7741 ttctatcgct gccactctcg tgttccttga tactcataat ggcttttccg ccatgcgaaa
     7801 caatttcatc atatatcaaa atactatcgg ttaccacaaa aacatcgtca aaaagttgcg
     7861 tgttttttgc cgcttcatac gtgcgttgaa taacggtttt tccacccaaa tcttgcagta
     7921 acttggcagg aaaacgtgta gaagcataac gagcaggaat aacggcgatg attttcattg
     7981 gaatacaatt ttaaagtggc taagttacaa agtttgcatc atttttttgg aagtttatca
     8041 tttggcttta tttttttgtg tggcaaaatg gtttaaaagt gaaaagaacc tttcaaactg
     8101 cttgggaagc ttcttttcaa tgttgagagc cttccaaact acttgggaag catgttttac
     8161 aataaaaaga acctttcaaa ctgcttggga agcttctttt caatgttgag agccttccaa
     8221 actacttggg aagcatgttt tacaataaaa agaacctttc aaactacttg ggaagcttct
     8281 ttttaatgct gagaaccttt tgccgaatga cggaaggttc ttttaaagtg ttgagagcct
     8341 tttgccaact gacggaaggt tcttttaaag tgttgagaac ttttcaaact acttgggaac
     8401 attgttttga tattaaaaac ttcttaaaag acgaacctta tatgattgga gttattggaa
     8461 tattagtttt aagtgcaata atataatcta ttgataaaac tttactcctc aaaactttcc
     8521 tctttaaacc caattaaata caatttgtct ttggcacgt
//
LOCUS       DAOOBH010000098         8250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_10538_length_8250_cov_5.167541, whole genome shotgun sequence.
ACCESSION   DAOOBH010000098 DAOOBH010000000
VERSION     DAOOBH010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10538_length_8250_cov_5.167541"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1766
                     /locus_tag="PK218_09290"
     CDS             267..1766
                     /locus_tag="PK218_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98743.1"
                     /translation="MKTITFASVLFFLSAISFAQVPSIEWQKSFGGTNQEYEHCIRQT
                     TDGGYIIVGETVSPVGGMITNSYGSYDCWVVKMNATGGLEWQKNLGGSGYDRANSVQL
                     TADGGYIVVGVTSSNNYDVIGHYGGSDVWVVKLSSTGVIEWQKPLGGSSEEEGFSIQQ
                     TTDGGYILCGYTFSTNGAVTTPTGGWIVKLNSTGTIQWQKGIGNYMNCIQQTTDGGYV
                     AAGYTYGPLGNTDYHIVKLNSSGVTQWQKYYGGTSIDMAEYIEQTTDGGYVVAGSSDS
                     SNTNVTNNNGSDDYWILKLNSLGELEWQKALGGSTYDYGKSVHQTSDGGFITCGYVNS
                     SNGMVTGNHGDADYWVVKLDNLGTVLWQKTLGGSGTDWGRSIQQTTDGGYIVAGTTGS
                     YSGDVTVQYGDRDFWVVKLSPDNLSTIDFSNENKVTIYPNPTKDYLKLSNYKNIQKVE
                     VYDLNGKRLILKNDFKDEKIVFQNFSKGIYTLKIYLNNGTIISDKIMKE"
     gene            complement(1860..2765)
                     /locus_tag="PK218_09295"
     CDS             complement(1860..2765)
                     /locus_tag="PK218_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPW98744.1"
                     /translation="MRALVISGGGSKGAFAGGVAQYLLQENGNKYDILIGSSTGSLLI
                     PHLALGKVEKIYNIYSNVDMRRIFNLSPFIVKNKNTDKEIVTINHLNVIKQFFNKKRT
                     FGESTNLLDYIKESFTVEEFKALQQAACDVIVTVTNFSKNEVEYKSIDDFEYDDFCEW
                     IWISCNYVPFMSLATKNGFDYGDGGFSSLVPIREAILRGAKEIDVIILETEVQVKPKS
                     ISRNPFTLMIDLFQILLTQVEKQNITIGKLAAKNKSVKLNLYYTPTQLTDNALIFNKE
                     KMKAWWQQGYDYAKNKSEIMSDNVQ"
     gene            complement(2762..4834)
                     /locus_tag="PK218_09300"
     CDS             complement(2762..4834)
                     /locus_tag="PK218_09300"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPW98745.1"
                     /translation="MRLLVFLLLSTFSFAQQIQKVDFKTAKGNIVINPKEKKVFGSVR
                     YVFTVKETIDTIKIDAVKMNFTSVMINKKEVKFTNSGKTLNLFEGFKKGKNTVTFDYE
                     AFPKQTMYFTGVEDNMQIWTQGQGKYTSHWFPSFDDVNEKVIFDMNVTFDKKYNVIAN
                     GFLKTTYTSGDSKKWYYEMKKPMSSYLLALAVGDFRNKVIASKSRTPLQFFIQPKDTA
                     KFEPTYRYSKQIFDYFEKEIGVKYPWKIYKQIPVEDFLYAGMENTTATIFAQDFVIDS
                     IGFNDRNYVNVNAHELAHQWFGDLVTAKSGKHHWLQEGFATYYALLAEREVFGEDYFY
                     HQLYRSSLQLRNAAKTDTIPVMNEKASSLSFYQKGAWALHYLRESIGAKAFQKAVKCY
                     LNKYKFKNVETDYFLAEIKKVAPTFDTANFKKTWLEDYRFQMDVANGLLRKNQFITTL
                     FETQQLRKKTFAENKSQFIEILQSDVYYPVKTEILYQIREISFEEKAPLLRLALQTND
                     IKVRQAVAEFTNDIPAEFKTEYETLLSDDSYETKEIAFMNLWKNFPENRAIYLEKAKD
                     WVGGNDKSLRILYITFALESDDLSYEDQTVYFEELLNYTSANYESSVRQNAFVSLFSM
                     NVDVEILLKNLVNATTHHKWQFTKYARDKIRNLISNEKYRKEFESILVTLNEAEKNKL
                     QNLLNEKP"
     gene            5170..5931
                     /locus_tag="PK218_09305"
     CDS             5170..5931
                     /locus_tag="PK218_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPW98746.1"
                     /translation="MITVAVIGAGNVAQHLILAFQKAKDIQLVQVLARKPKQLTHLLG
                     SHGIVTEYSQLKEVDLYIIAVSDNAIATVSSQIPFKNKLVVHTSGSMELNVLDSKNRK
                     GVFYPLQTFSKAKPLDFSQIPIALEAENESDYTVLETVANAISTQVHHINSEQRKALH
                     IAAVFVCNFTNHLYKIGNDICIENNLSFDLLKPLILETADKIITLSPTDAQTGPAKRE
                     DTVTINAHLNFLKNHTQKDIYKLLTQSIIDNGKKL"
     gene            5915..6442
                     /locus_tag="PK218_09310"
     CDS             5915..6442
                     /locus_tag="PK218_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPW98747.1"
                     /translation="MEKSYKELMNHITTFIFDIDGVLTDSSVHVTESGEMLRIMNIRD
                     GFAMKAAIESGYHVCIISGGKNEGVRIRLRNLGITDIHLGSPDKVETFKEYIELYNIK
                     PEQVVYMGDDIPDYHVMQLVGLPTCPQDASSEIKAISKYISHKNGGKGAVREVIEQVM
                     KVQGKWHMYYNGKHD"
     gene            6532..7473
                     /locus_tag="PK218_09315"
     CDS             6532..7473
                     /locus_tag="PK218_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HPW98748.1"
                     /translation="MKFLNLIRYKNLLLIALMQLLFRYGFLAQQDVPLVLKDWQYALL
                     VLATVLIAAAGYVINDIMDQETDLTNKPNDVIVGKSISEDTAYYIYFALTISGVLIGY
                     YLSNYVNKTSFFGIFIIISVLLYLYATSFKQIAVVGNLIVAFVLGLSVVIIGMFDIIP
                     LLSFVSADQSINLQTLLGIILDYAIFAFFINFIREIVKDLEDIDGDYNQGMNTLPIAI
                     GKERTAKIVSVFAAIATLVLLWYVNKNLMDSKLFWASAYCLLLVISPMLFITVKSWTA
                     KSKKEFRLLSSVLKMIIFFGILSVLIISLNIKYNVKG"
     gene            7460..8044
                     /locus_tag="PK218_09320"
     CDS             7460..8044
                     /locus_tag="PK218_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HPW98749.1"
                     /translation="MLKDKLKNYKITLASGSPRRQQFFRELGLDFEIRLKEVEEIYPE
                     NLQGVEITNFLAFLKASAFDNDINKNEIVITSDTIVWLDNKALGKPKDFNHALEMLLQ
                     MTGKTHEVITSVCFKTASKMDILNCITKVTFSQITEKEIRYYLDVFKPFDKAGSYGIQ
                     EWIGLIGISRIEGSYTNVVGLPTEMVFNYLKNLE"
     gene            8041..>8250
                     /locus_tag="PK218_09325"
     CDS             8041..>8250
                     /locus_tag="PK218_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPW98750.1"
                     /translation="MTFFENYIKQIFATGILIILMIITRLLMTRVIKNYAKNHTNLEH
                     RTNLVIKYVHLLINILTIIALIIIWG"
BASE COUNT         2808 a         1342 c         1339 g         2761 t
ORIGIN      
        1 tttttctaac ttttagttgc attagttgca gttggatggt tggttttttg acaacccaga
       61 taacgcacta atcctttcgt gtggttcaac aaccaaacct gtcaggttta caaacaaaaa
      121 gtgataatca ctgcatatta tcactcatta actcgctttt gtcccgactg cgcattctat
      181 gatgaaaaac aaaagacttg taaaggattg ttcaacttat ttaaaaaaag aatactttta
      241 acaaataaaa atttaactac atcctaatga aaacaataac ctttgcttca gtactctttt
      301 ttttatcggc aattagcttt gctcaagttc catccattga atggcaaaaa tcatttggtg
      361 gcacaaatca agagtatgaa cattgtataa gacaaactac tgatggtggt tacataattg
      421 tgggcgaaac ggtttctcct gttggtggta tgattacaaa tagctatggt agttatgatt
      481 gttgggtagt aaaaatgaat gccactggtg ggcttgaatg gcaaaaaaac ttaggcggtt
      541 ctggatatga tagagctaac tctgttcaac ttactgccga tggtggttat atagtagttg
      601 gagtaacaag ttctaataat tatgatgtaa taggccatta tggcggtagt gatgtttggg
      661 tagtaaaatt aagtagtaca ggagttatag aatggcaaaa accattaggt ggttcgagtg
      721 aagaggaagg ttttagtatt cagcaaacta ctgatggtgg gtatatttta tgtggctata
      781 ccttttctac aaatggcgcc gttactacgc caacaggtgg ttggattgta aaattaaaca
      841 gtacaggtac aattcaatgg caaaaaggaa ttggcaatta tatgaattgt attcaacaaa
      901 ctaccgatgg tgggtatgtt gctgctggct atacgtatgg tcctctaggt aataccgatt
      961 atcacatagt aaagcttaac agttctggcg ttacacaatg gcaaaaatat tatggtggta
     1021 cttcaattga tatggcagaa tacattgagc aaacaactga tggtggttat gtcgttgccg
     1081 gatcaagcga ttcaagtaac actaatgtaa ctaataataa cggaagtgat gattattgga
     1141 tactaaaatt aaacagtttg ggagagttag aatggcaaaa agcattaggc ggttcaactt
     1201 atgattatgg gaaaagtgtt catcaaactt ctgatggcgg ttttattact tgtggctatg
     1261 tcaattcgtc taacggaatg gttacaggta atcatggcga tgctgattat tgggtagtaa
     1321 agttggacaa tcttggaact gtcctgtggc aaaaaacgtt aggtggatct ggaacagatt
     1381 ggggacgaag tattcagcaa actactgatg gtggttatat agttgctggt accacaggtt
     1441 cttacagtgg agatgtcaca gtacaatatg gtgatcgcga tttttgggta gtaaaactta
     1501 gtccagataa tttaagtacc attgattttt caaatgaaaa taaagtaact atttatccca
     1561 atccaacaaa agattattta aaattatcaa attataaaaa cattcaaaag gttgaagtat
     1621 atgatctaaa tgggaaaaga ctaattttaa aaaatgattt taaagacgag aaaatagtct
     1681 tccaaaactt ttcaaaaggt atatatacac taaaaattta tttaaataac ggaactatta
     1741 tatcagacaa aatcatgaag gaataaattt aatgtttttt atctaataca aagtaaaagt
     1801 gcgaaatcgt tcgcgtagtc aaacaaaatt agtaatagag attacattcg tgggttatgt
     1861 tactgcacat tatcactcat tatttcactc ttgtttttgg catagtcata tccttgctgc
     1921 caccaggctt tcattttttc tttattaaaa ataagtgcgt tatccgttag ttgtgttggc
     1981 gtataataaa gattgagttt tacactttta tttttggcag ccaattttcc aatagtaata
     2041 ttttgtttct ctacttgtgt tagtagtatt tgaaacaaat ctatcattaa tgtaaatgga
     2101 tttctgctga tacttttggg ctttacctgt acttctgttt ccaaaataat aacatcaatt
     2161 tcttttgcac ctctaagaat ggcttctcgt attggaacca aactcgaaaa tccgccatcg
     2221 ccataatcaa aaccgttttt ggtagccaaa ctcataaaag gtacataatt gcaagatatc
     2281 caaatccact cgcaaaaatc atcgtattca aaatcatcaa ttgatttgta ttctacttca
     2341 ttttttgaaa aattagtgac tgttacaatt acatcgcatg ctgcttgttg tagtgcttta
     2401 aattcttcaa ccgtaaagct ttctttaata tagtctaaaa gatttgtgct ttcgccaaaa
     2461 gttctctttt tattaaaaaa ttgttttatt acgtttaaat gattaattgt aactatttct
     2521 ttatccgtat ttttattctt tacaataaaa ggcgacaaat tgaatatccg tcgcatatct
     2581 acgttagaat aaatattgta aattttctcc acttttccca aagctaaatg tggaatgagt
     2641 aaactaccag tagaacttcc aatcaaaata tcatatttat tgccgttttc ttgtaataaa
     2701 tattgagcaa caccaccagc aaaggcacct ttgcttccac cgcctgaaat tactaatgct
     2761 ctcatggttt ttcgtttaaa agattctgta atttattttt ttcagcttca ttcaaagtta
     2821 ctaaaatact ttcaaactct tttctgtact tttcatttga gattagattc ctaattttat
     2881 ctcttgcata tttagtaaac tgccacttgt gatgagttgt agcatttact aaatttttta
     2941 atagaatttc aacatcaaca ttcattgaaa acaaagaaac aaacgcattt tgacgcacgc
     3001 tactttcgta gtttgccgaa gtataattta gtaattcttc aaaataaaca gtttgatctt
     3061 cataactcaa atcgtcactt tcaagagcaa aggttatata taaaatgcgt aaacttttat
     3121 cgtttccacc aacccaatct tttgcttttt ccaaataaat agctctattt tctggaaaat
     3181 ttttccacaa attcataaac gctatttctt tggtttcata agaatcatcg ctaagcaatg
     3241 tttcatattc agttttaaat tctgcaggaa tatcattcgt aaactcggct acagcttgtc
     3301 gaacttttat atcattggtt tgcaatgcca atcgcaaaag tggtgctttc tcttcaaagg
     3361 aaatttctct tatttgatat aagatttccg ttttaacagg ataataaaca tctgattgaa
     3421 ggatttctat aaactgtgat ttattttcag caaatgtttt ttttcgcaat tgttgggttt
     3481 caaaaagtgt tgttataaat tgattttttc taagtaaacc attcgcaaca tccatttgaa
     3541 aacgataatc ttctaaccac gttttcttaa aattagcagt gtcaaatgtt ggtgctactt
     3601 tttttatttc agccaaaaaa taatcggttt caacattttt aaatttatat ttattcaaat
     3661 aacacttcac cgctttttga aaagcctttg ctcctattga ttctctcaaa taatgcaaag
     3721 cccaagcacc tttttgataa aacgacaacg aactcgcttt ttcattcatc accggaatgg
     3781 tatcggtttt ggcagcattt ctaagctgta aagacgaacg atacaattgg tgataaaagt
     3841 aatcttcgcc aaaaacttcc cgttcggcaa gcaaggcata ataggtcgca aaaccttctt
     3901 gcaaccaatg atgtttgccc gatttggcag taactaaatc gccaaaccac tgatgcgcca
     3961 actcgtgtgc attaacattt acgtaatttc tgtcgttaaa cccaatgctg tctatgacaa
     4021 aatcttgtgc aaaaatagtt gccgtagtat tttccattcc agcataaaga aaatcttcca
     4081 ccggaatttg cttgtagatt ttccagggat acttcactcc tatttccttt tcaaaataat
     4141 caaagatttg tttcgaatag cgatatgttg gttcaaactt cgcagtgtct ttgggctgaa
     4201 taaaaaactg caacggcgtt ctagatttag aagcaataac tttatttcta aaatcaccaa
     4261 cagccaatgc caataaatag gaactcattg gttttttcat ttcataatac cacttttttg
     4321 aatctccaga agtgtaagtc gttttcaaaa aaccattggc gatcacatta tattttttat
     4381 caaaagtaac attcatatca aaaattactt tctcattcac atcatcaaaa ctcggaaacc
     4441 aatgactggt atattttcct tgaccttgcg tccaaatctg catgttgtct tctacacctg
     4501 taaaatacat cgtttgttta gggaacgctt catagtcaaa agtcaccgtg ttctttcctt
     4561 ttttaaaacc ttcaaacagg tttagcgttt tgcccgaatt ggtaaatttg acctcttttt
     4621 tgttaatcat taccgaagta aaattcattt ttacagcatc aatcttaatc gtgtcgatag
     4681 tctcttttac ggtaaaaaca tagcgcaccg aaccaaaaac ttttttctct ttgggattaa
     4741 tgacaatgtt tccttttgcg gttttaaagt ccactttttg aatctgttgt gcaaaagaaa
     4801 aagttgagag taataaaaaa actagtaaac gcattagtga tagattatga tgttacgaat
     4861 ataaacattt tgatagtagc ctattttcta aatttaaaaa ttaacacaat acatttaaaa
     4921 tgatggaaaa aagactattc atctataaaa aatattaatt tgacaattat tcctctaaaa
     4981 aataaaaaac taaaatctat tttaatggaa tgatatttct aaaaatctta aaaatgacaa
     5041 aaaccttgaa gataaattga gagaatattg taaagatttt tttttagtga aataaaaaag
     5101 aacataaagt tagattttca ttccctaaaa ttctttttaa atacctgcta tttttagtac
     5161 ttttgctaaa tgataacagt agccgttatt ggtgcgggta atgtagccca acatttgatc
     5221 ttagcttttc aaaaagccaa agatattcag cttgttcaag tactagcacg aaaaccaaaa
     5281 caactcactc atctacttgg ttcgcatggt atcgttactg aatacagcca attgaaagaa
     5341 gtcgatttat acatcattgc cgtttcagac aatgctattg ctactgtttc aagtcaaata
     5401 ccattcaaga acaaactagt tgttcacact tcgggcagta tggaacttaa tgtattggat
     5461 agcaagaacc gtaaaggtgt tttttatccg ttgcaaacat tttctaaagc aaaaccttta
     5521 gattttagtc aaatacccat tgctttagag gcagaaaacg aaagtgatta tactgtgctt
     5581 gaaaccgtag ccaatgctat ttcaactcaa gtacatcaca tcaattccga gcaacgtaaa
     5641 gctttacaca tagccgcagt gtttgtgtgc aatttcacca accatttgta taaaatagga
     5701 aacgatatat gtatcgaaaa caacctgtcg tttgatttac taaaaccatt aatcctagaa
     5761 actgccgaca aaataataac cctatcgcct actgatgcgc aaactggacc agcaaaacga
     5821 gaagatactg ttaccataaa cgcacatcta aattttctaa aaaatcatac ccaaaaagac
     5881 atctataaac tcctaaccca atcaatcatt gacaatggaa aaaagctata aagaactgat
     5941 gaaccacatt accactttca ttttcgatat tgatggtgtg ctaaccgaca gctctgttca
     6001 tgtaaccgaa agtggagaaa tgcttcgcat catgaacata cgagatggtt ttgctatgaa
     6061 agcagctata gaaagtggct atcatgtgtg tatcatttct ggtggaaaaa acgaaggcgt
     6121 acgtattcga ttgagaaatc tcggcattac cgacatccat ttgggttcgc cagacaaggt
     6181 ggaaacattc aaagaatata tagaattata caatatcaaa cccgaacaag tagtctatat
     6241 gggtgatgac attcccgatt atcatgtaat gcaattagta ggattaccaa cctgtccgca
     6301 agatgccagt tctgaaatta aagctatctc caaatacatc tcgcataaaa atggtggaaa
     6361 aggtgctgtg agagaagtaa ttgaacaagt gatgaaagtt caaggtaaat ggcacatgta
     6421 ttataatggg aaacacgatt aagaagcagg aagttgggaa ttagaagctg gaagttttaa
     6481 accaatgcta aactcataac tttataaact ataaacttac aaccttaaaa aatgaagttc
     6541 ttaaacctaa taagatacaa aaacctactc ctaattgctt taatgcaatt attatttcga
     6601 tatggcttct tagcacaaca agacgttccg ctggtattaa aagattggca atatgcatta
     6661 ttagtattgg caacggtatt gattgcagca gcaggttatg ttattaatga cattatggac
     6721 caagaaaccg acctaacaaa caaaccaaat gatgtaattg ttggcaaatc tatttcagaa
     6781 gatacagctt attatatcta ttttgcacta acaattagtg gtgttttgat aggctattat
     6841 ctttcaaatt atgtcaacaa gacatcattt ttcggaatat ttattattat ttcagtatta
     6901 ctttatttgt atgctactag ttttaaacaa atagcagtgg taggcaattt aattgtagcc
     6961 tttgttttag gattaagtgt tgttattatc ggaatgtttg atattattcc gttattatca
     7021 tttgtaagcg ccgatcaaag tatcaatcta caaacacttt tagggattat tttagactat
     7081 gcaatttttg cttttttcat taacttcatt cgagaaatag ttaaagatct tgaagatatt
     7141 gatggtgatt ataaccaagg aatgaacacc ttacctattg ctattggaaa agaaagaacc
     7201 gccaagatag tttctgtttt tgccgctata gctacattag ttttattgtg gtatgtaaac
     7261 aaaaacttaa tggattctaa acttttttgg gcttctgcct attgtctatt actcgtaatt
     7321 agcccaatgc tatttattac agtaaaatca tggactgcaa aatctaaaaa agaatttcga
     7381 ttgttaagca gtgtcttaaa aatgataatc ttttttggaa tcctatcggt tttaataatt
     7441 tcgctaaaca ttaaatacaa tgttaaagga taaactaaaa aattacaaaa tcactttagc
     7501 ttcaggttct ccaaggagac aacagttttt tagagagtta ggcttagatt ttgaaatacg
     7561 tttaaaagag gtagaagaaa tctatcccga aaacttgcaa ggcgttgaaa taacaaattt
     7621 tttggcattt ttaaaagcaa gcgcttttga caatgatatt aataaaaatg agatagtaat
     7681 taccagcgac accattgttt ggttggataa caaagcttta ggcaaaccaa aagatttcaa
     7741 ccatgcatta gaaatgctgt tacaaatgac aggaaagact catgaagtca ttacatccgt
     7801 atgttttaaa acagcatcta agatggatat tttaaactgt atcactaaag ttaccttcag
     7861 tcaaataact gaaaaagaaa tacgctatta tttagatgta tttaagcctt ttgataaagc
     7921 tggtagctat ggtattcaag aatggattgg tttaattggt atttcaagaa tagaaggttc
     7981 ttacaccaat gtagttggat taccaacaga aatggttttt aattatttaa agaatttaga
     8041 atgacatttt ttgaaaacta tattaaacaa atatttgcga ctggaatttt aatcatcttg
     8101 atgattatta caagattatt gatgacaaga gtaataaaga attatgccaa aaatcatact
     8161 aacttggaac atcgaaccaa tttagtaatt aaatatgttc atttactgat taatatttta
     8221 acaataatag cattaataat catttgggga
//
LOCUS       DAOOBH010000099         8196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_10611_length_8196_cov_6.141383, whole genome shotgun sequence.
ACCESSION   DAOOBH010000099 DAOOBH010000000
VERSION     DAOOBH010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10611_length_8196_cov_6.141383"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="PK218_09330"
     CDS             complement(<1..1708)
                     /locus_tag="PK218_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HPW98751.1"
                     /translation="MNVNYNWRMSNRLSANQKSKQSEIECKKRSFSQPTNWLLMALLL
                     MFSFSFAQTIQLGTGTSTNSNVPINYNWGYNYTQTIYTAAEMTAAGASATGTITSIKF
                     KPTSSVSTALWKDWVVYIGNTSKDGFTSTTDWIPLASLTEVFNGTLIANTVANSWMEI
                     TLATPFNWTGGNLVVAIDENTASYGGNPNWASYTLAPTTGNKSIYYRNDSTNPSPSTP
                     PTASSRSNVVAQIQFVFPSVTVPNCAISHAPAAAATGVARNATLSWADGGGGPTSYDV
                     YFGTSTSPALVGNQVGTSYTPSLMAANTSYYWRVVPKNANGEAVGGCTERSFTTGTGY
                     NYCSVSVTNTGDYTSAFSTTLATTNISYTAGSNPADSYTDQTAQSFQVGQGTSFNFSH
                     TYVGGSNGVKAWVDYNSDGTFGAGEDVYFLADSNATKNGTITVPLGTPLGTYRMRVRS
                     QFGSTAEPTACGNLSYGTTMDYTLTVIAPPSCLAPSALASSAVTASSATLSWTASASS
                     PANGYDIYYSTTNTAPLAGTTPTVDNHTASPYNVSGLTSATTYYWWVRSDCGAETSAW
                     ASG"
     gene            complement(1943..2584)
                     /locus_tag="PK218_09335"
     CDS             complement(1943..2584)
                     /locus_tag="PK218_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98752.1"
                     /translation="MQNLKNIAVGFLVSFIGSIPLGYLNIVGYEIYNKSGIISTSLYL
                     LGVICVEFLVIFGTLFFADKLNKNEKLLKTIELFSILFMFVLAYVFYTNGNDEQTNKT
                     IFSTVSQNFFFSGILFSSLNFIQIPFWLSWNLYLVNRDYIEISKNRKYFYVFGTLVGT
                     FLGMLILILSLYYFANQVDFLSRYLMKIIIPLVFVLLGILQTVKFYKKFKKKQ"
     gene            complement(2584..3624)
                     /locus_tag="PK218_09340"
     CDS             complement(2584..3624)
                     /locus_tag="PK218_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPW98753.1"
                     /translation="MNVKLFFHKLTHWEYWPFQIVYIPIYFLWAFYALRQRTIFFFNT
                     CNPTMRNGGFIMDSKKEIYDLIPKKYYPTTEFVKENCPFDEVIAIKQNSNIQFPLIAK
                     PDVGLRGSAVQKIESKEALKSYHQKANFDYIIQDLIPYKNEVGIFYIRFPNQKKGKIT
                     GIVAKEFLIVEGDGKSTVLDLILKNPRYEMQLKALMKEYGWGLNIILEKGEKRNLVPF
                     GNHIRGAKFIDASDKISESLTKVIDEMCLQIDEFYFGRMDVMYNTWEELEQGINFQVV
                     ELNGAASEPTHIYDPMHSLFFAWKELARHITYMYRISVQNHQRGFPYLSHSEGIKQYR
                     LHLTQSKKIVNF"
     gene            complement(3636..4346)
                     /locus_tag="PK218_09345"
     CDS             complement(3636..4346)
                     /locus_tag="PK218_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HPW98754.1"
                     /translation="MCTVSFVRSNNKFIITSNRDEQVLRPSAIEPKNYCINNKNIIFP
                     KDKKAGGTWYAVDEYSNVLVLLNGANDKHLQKEDYRKSRGIIVLDLIGSNSPIAQWNA
                     IDLDNIEPFTIVLFENRRLYQLQWNEKEKSTLELDVKQSHIWSSSTLYSSEIREKRKQ
                     WFYTFLDTKPEISASEMLTFHRYTEQENTENGLVINRNDFLKTLSITQTVLENNKVEI
                     HYADLQLNKEFSTAFITV"
     gene            complement(4334..4822)
                     /locus_tag="PK218_09350"
     CDS             complement(4334..4822)
                     /locus_tag="PK218_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPW98755.1"
                     /translation="MLIPSIQKTLNELLELIHQLSDEDYTKPCEALSNATIGEHTRHI
                     IEMFQCLENQYVNGVVDYDKRERNLQIQTEAKFASNAIVTILNEIDKPNKELSLEQCV
                     DGEILLIQSNYKRELLYNLEHCIHHQALIKVALLHYGITAVDKNFGVARSTIEYRKQC
                     VQ"
     gene            complement(4889..5272)
                     /locus_tag="PK218_09355"
     CDS             complement(4889..5272)
                     /locus_tag="PK218_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPW98756.1"
                     /translation="MKVVVIIVKLLIVIIVGQTLFFKFTGASESIYIFEQLGAEPYGR
                     IGSGIVELLASILILIPRTTLLGALLAFATMLGAIASHLFVLGINVQNDGGTLFVLAL
                     VTATASLFLIYQERNKIPDLLKFKI"
     gene            complement(5306..5899)
                     /locus_tag="PK218_09360"
     CDS             complement(5306..5899)
                     /locus_tag="PK218_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HPW98757.1"
                     /translation="MKKIIILLAIAFTQLSIAQNDLPKSATDIAPLLIGEKAPNTSLK
                     TIDGKSVSFLETLDKKKTILVFYRGGWCPYCNQHLSALAEAEPELIKLGYKIIAISPD
                     SSKSLQETKSKDEVNYTLLSDSKNNFSKAFGIAFEAPKNYEKYLLKGSDNVNSSIIPV
                     PSVFILDKEGTILFEYIAPDYKHRLSNELLMAVAKQF"
     gene            complement(5986..6573)
                     /locus_tag="PK218_09365"
     CDS             complement(5986..6573)
                     /locus_tag="PK218_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPW98758.1"
                     /translation="MGFKTAKKGDVIYFSSSDVPRVFVLKKGAIKIVSLDEDGNETIK
                     DIIQKGDLFGELELESDKDSNEYAKALTSEVSICSFLLSDFENLLLKYPNLALSYTKF
                     VGLKMKRLKNSYANLMSKDARTRLLTFLKDWSEREGRKEGNKITIDNYLTQNDIAQII
                     CTSRQTATLLLKELEENGLMYYGRKEIIIEDISKL"
     gene            complement(6748..7884)
                     /locus_tag="PK218_09370"
     CDS             complement(6748..7884)
                     /locus_tag="PK218_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPW98759.1"
                     /translation="MDFNLTEEQLMIQQAAKDFAQAELLPGVIERDEHSKFPTEQVKM
                     MGELGFLGMMVDPKYGGSGLDSVSYVLAMTEIAKVDASAAVIMSVNNSLVCAGMEKYC
                     NEEQKMKYLVPLAKGEVIGAFCLSEPEAGSDATSQKTTAIDKGDHYLLNGTKNWITNG
                     ATASTYLVIAQTDVEKGHKGINAFIVEKGWKGFDIGPKEKKMGIRGSDTHSLMFSDVK
                     VPKENRIGADGFGFSFAMNVLNGGRIGIASQALGIATGAYELALKYSQERKAFGKEIF
                     KHQAIAFKLADMFVKVTAAKLLVLKAACEKDEGKDIAHSGAMAKLYASEIALEVANEA
                     VQIHGGNGYVAEYHVERMMRDSKITQIYEGTSEIQRIVISRGLI"
     gene            7991..>8196
                     /locus_tag="PK218_09375"
     CDS             7991..>8196
                     /locus_tag="PK218_09375"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPW98760.1"
                     /translation="MSKESYKVVGVMSGTSLDGIDLAHLDFSIEDGKWSFKVHECETI
                     AYPKEWLNKLKIAVSFSKDALDDL"
BASE COUNT         2567 a         1435 c         1319 g         2875 t
ORIGIN      
        1 cccctgaagc ccaagcacta gtttctgctc cacaatcaga acgtacccac cagtaataag
       61 tagttgcaga agttaatcct gaaacattat aaggtgatgc cgtatgatta tcaactgttg
      121 gtgttgttcc agctaaaggc gcagtatttg tagtactata ataaatatca taaccatttg
      181 caggactact tgcagaagcg gtccaactta aagtcgctga actagctgtt actgctgaac
      241 tagctagtgc tgatggtgct aaacaagatg gaggtgcaat aacagttaat gtataatcca
      301 ttgttgtacc ataactaaga tttccgcagg cagttggctc agccgtacta ccaaattgcg
      361 atctaacacg cattctatac gttcccagcg gcgttcccaa cggaacagta attgtgccgt
      421 tttttgttgc gttagaatcc gctaaaaaat aaacgtcttc accagctcca aatgttccgt
      481 cactattata atcaacccaa gccttaacac catttgatcc acctacataa gtatgcgaaa
      541 aattgaatga agttccttgt cctacttgaa agctttgcgc agtttggtcg gtatatgaat
      601 cagctgggtt gctacccgca gtatatgaaa tattagtagt cgctaaagtg gtactaaaag
      661 ctgaagtgta atcgccagta ttagttacac ttactgaaca ataattatat cctgtgcctg
      721 tagtaaatga gcgttctgtg cacccaccaa cggcttcacc attagcgttt tttggaacaa
      781 ctctccaata gtaagaagta ttagcagcca ttagagacgg cgtataagat gtacccactt
      841 gatttcctac taatgcagga cttgtagatg ttccaaaata aacatcataa gatgttggtc
      901 caccacctcc atctgcccaa gacagagtag catttctagc tactcctgtc gcagcagcag
      961 ctggagcatg agaaatagca cagtttggca ccgttacaga tggaaaaaca aactgaattt
     1021 gagcaacaac atttgatcta cttgaagctg ttggaggtgt actaggactt ggattagtcg
     1081 agtcattgcg ataatatatc gatttatttc ctgtcgttgg cgctagcgta taacttgccc
     1141 aatttggatt accaccataa cttgccgtat tttcgtctat agcaaccact aaattaccac
     1201 cagtccaatt gaaaggcgta gctaaagtga tttccatcca tgaattggct acagtattag
     1261 caattagagt tccattaaat acttctgtta aacttgcaag tggaatccaa tctgtagttg
     1321 aagtaaaacc atctttagac gtatttccaa tatatacaac ccagtctttc cacaatgcgg
     1381 tagaaactga cgatgtaggt ttaaatttaa ttgaagtgat tgttccagta gcagaagctc
     1441 ccgcggcagt catttcagcg gcagtgtaaa tggtttgtgt ataattataa ccccagttat
     1501 aatttattgg aacattggag tttgttgaag tccccgtacc aagttgaatc gtttgagcaa
     1561 acgaaaaaga aaacatcaaa agcaacgcca tcaacaacca atttgttggt tgtgaaaaac
     1621 ttcttttttt acattcaatt tctgattgct tacttttttg atttgcggaa agcctatttg
     1681 acatccgcca attgtagttt acattcataa tttattgttt ttataattca ctactaaagg
     1741 tacagctatt attgggttaa caataaatta acttcgtcaa atcgatgaaa aacaacaaat
     1801 catcgataaa aatcatgcct tacaggcgaa ttatcataat ttcatcctgt tttttggcaa
     1861 ttattcactt tttaggcatt ttttttaaac atgtatttta atagttttta aacctaaaaa
     1921 agaatctagc attgagaaaa tcctactgtt tttttttgaa ctttttgtag aatttaactg
     1981 tttgaagtat tcctaacaaa acaaaaacaa gaggtataat aattttcatt aaatacctgg
     2041 aaagaaaatc aacttgatta gcaaaataat ataaagacag gattaaaatc aacatcccta
     2101 aaaaagttcc aacaagtgtt ccgaaaacat aaaagtattt tctgtttttt gatatttcaa
     2161 tataatctct atttactaaa tacaaattcc agctgagcca aaaaggaatt tgaataaagt
     2221 ttaaactact aaagaggatt ccagagaaga agaagttttg agaaacagta gaaaaaattg
     2281 tcttattggt ttgctcgtca tttccattgg tgtaaaacac ataggctaaa acaaacataa
     2341 aaagtatcga aaacaattca attgttttta ggagtttttc atttttattt aatttatctg
     2401 cgaaaaacaa agttccaaaa atcactaaaa actctacgca aattacacct aataaatata
     2461 acgaagtact aatgattcca gatttgttgt aaatttcata acctacaata ttcaaatagc
     2521 ccaaaggaat agagccaatg aaactgacta aaaatccaac ggctatgttc tttaggtttt
     2581 gcattaaaag tttacaattt ttttggattg tgtaaggtgc aaacggtatt gttttattcc
     2641 ttcactatga gaaaggtacg gaaatccgcg ttggtggttt tgaacactaa ttctgtacat
     2701 ataagtaatg tgtcgtgcaa gctctttcca agcaaaaaaa agagaatgca ttggatcgta
     2761 aatatgtgtt ggttcactag ctgctccatt caattctaca acttgaaagt ttattccttg
     2821 ttccaattct tcccaagtat tgtacataac gtccatgcga ccaaaataaa actcgtcaat
     2881 ttgcaaacac atttcatcaa ttacttttgt taaggattct gaaattttat cgctggcatc
     2941 aatgaacttt gcgcctcgaa tatgatttcc aaacggcact aaattgcgtt tttccccttt
     3001 ttctaaaata atatttaatc cccaaccata ctctttcatc aatgctttta actgcatttc
     3061 atatctcgga ttttttaaaa ttaagtctaa aactgttgac tttccatctc cttcaactat
     3121 taaaaattct ttggcaacaa tacctgtaat ttttcctttt ttctggtttg gaaatcgaat
     3181 gtaaaaaatc ccaacttcat ttttatatgg aattaagtct tgaataatat aatcaaaatt
     3241 agctttttga tggtaacttt ttaacgcctc tttggattca attttttgaa ctgccgaacc
     3301 acgaagccca acatctggtt ttgctattaa tggaaactga atattgctat tttgtttaat
     3361 ggcaattacc tcatcaaatg gacaattttc ttttacaaac tcagttgttg gataatattt
     3421 tttgggaatt aaatcataaa tttctttttt gctatccatg atgaatccgc catttcgcat
     3481 cgttggatta cacgtattaa aaaagaaaat cgttcgttga cgtaaagcat aaaaagccca
     3541 aagaaaatag attggaatat aaacaatttg aaaaggccaa tattcccaat gggtaagttt
     3601 gtgaaaaaat agtttgacgt tcaatggctc aatgtttata ctgtgatgaa tgctgttgag
     3661 aattctttat tcaattgtaa atcggcatag tgaatctcta ctttgttatt ttctagaaca
     3721 gtttgtgtaa tactcaacgt ttttagaaaa tcatttctat tgatgactag tccgttttcg
     3781 gtgttttctt gttcggtgta gcgatgaaaa gtaagcattt ctgaggcact aatttcgggt
     3841 ttagtgtcta aaaaagtata gaaccactgt tttctttttt cgcgtatttc agaggaatac
     3901 aaggttgaag atgaccaaat atgagattgt ttaacatcta attccaaagt tgatttttct
     3961 ttttcattcc attgtaattg atacaatctt ctattttcaa acagcacaat agtaaatggc
     4021 tctatgttat ctaaatcaat agcattccat tgcgcaatag gcgaattact tcctattaaa
     4081 tctaaaacaa tgatgcctct actctttcga tagtcttctt tttgaagatg cttgtcattg
     4141 gcaccattaa gcaaaaccaa tacattagag tactcatcaa ctgcatacca agttccgcca
     4201 gcttttttat cttttggaaa gataatgttc ttattgttta tgcaatagtt tttgggttca
     4261 atggcacttg gtcggagtac ttgctcatcg cgattggatg taattatgaa tttattgttg
     4321 gaacggacaa aacttactgt acacattgtt ttctatattc tatggtagat cgtgctactc
     4381 caaaattttt atcaactgcc gttattccgt agtgtaacaa agcaactttt attaacgctt
     4441 gatggtgaat acaatgttct aaattgtaaa gtagctctct tttatagttg ctttgtatca
     4501 aaaggatttc gccgtcaaca cattgttcta agcttaactc tttgttgggt ttatctattt
     4561 cattcaaaat tgttactatc gcatttgatg caaacttagc ttctgtttgg atttgaagat
     4621 ttctttctct tttatcgtaa tcaactacac catttacata ttgattttcc aagcattgaa
     4681 acatttcaat gatgtgtcga gtatgttcac caatagtcgc attactaagt gcttcgcatg
     4741 gctttgtgta atcttcatct gaaagttggt ggattaattc caacaattcg tttaacgttt
     4801 tttgtataga aggtataagc attagtgtga tattttaaaa aatatacgaa actcattaag
     4861 atttagattt ggatttgaaa attagtgatt aaattttaaa ttttagtaaa tctggaattt
     4921 tatttctttc ttgatagatt aaaaatagac ttgctgttgc tgtaactaac gctaaaacaa
     4981 acaaggttcc tccatcattc tgaacattaa tacctaacac aaaaagatgt gaagctatag
     5041 caccaagcat agtagcaaaa gccaataaag ctccaagcaa agtcgttctc ggaattagta
     5101 tcaatataga agctaataat tctacaattc cagaacctat tctaccataa ggttctgcgc
     5161 ctaattgttc gaaaatgtag atgctttctg aagctccagt gaatttaaaa aacaacgttt
     5221 gtcctactat tataacaata agaagtttaa cgataataac tactactttc atggcaaacg
     5281 ctgtttttta tggttattaa tggaattaaa actgtttggc aaccgccatc aacaattcgt
     5341 tagacaatct gtgtttgtaa tcgggtgcga tatattcgaa aagaattgta ccttctttgt
     5401 ctaaaatgaa tacacttggc actggaataa tacttgaatt gacgttatca gagcctttta
     5461 ataaatattt ctcatagttt tttggagctt caaatgctat tccaaaagct ttggagaaat
     5521 tatttttgct atctgaaagt aacgtgtaat ttacttcatc ttttgattta gtttcttgta
     5581 aactttttga cgaatcagga ctaatagcta taattttata gcctaatttg attaattctg
     5641 gttctgcttc tgctaatgca cttaggtgct gattacaata cggacaccat ccgccacgat
     5701 aaaaaactag aattgttttc tttttgtcaa gcgtttcaag aaatgaaacc gattttccat
     5761 caatggtttt tagcgaagta ttgggtgctt tttctcctat taataatggc gcaatgtcgg
     5821 ttgcagattt tggtaaatcg ttttgagcta tacttaattg tgtaaaagca attgctaaaa
     5881 ggatgatgat ttttttcatg agtagtttat ttgtttttgt attacaaaga taattctact
     5941 ctaaaagtct aaatgtaagt tagtttacaa atgaagaaaa attaactaaa gttttgaaat
     6001 atcttcaatg attatctctt ttctaccata atacatcaag ccattttctt caagttcttt
     6061 tagcaataga gtagctgttt gtctagaagt acaaataatt tgagcaatat cattttgagt
     6121 taaataatta tcaatagtga ttttattccc ttcttttctt ccttctcttt ctgaccaatc
     6181 ttttaagaat gtaagtagtc gtgttcgggc atcttttgac atcaagttgg cgtaactatt
     6241 ctttaatctc ttcattttca gacctacaaa cttggtgtaa gacaacgcta aatttgggta
     6301 tttcaacaat aaattctcaa aatcagaaag taaaaagctg caaatagaaa cttcactagt
     6361 gagtgctttg gcatattcgt ttgaatcttt atcgctttct aattctagtt cgccgaacaa
     6421 atctcctttt tgaataatgt ctttaatggt ttcattgcca tcttcgtcta aggaaacaat
     6481 tttaatagca ccttttttaa gtacaaaaac ccttggaaca tcggaagaag aaaaataaat
     6541 aacatcgcct ttcttggcgg ttttaaatcc cataatcaga cacaactgct taatttgcgc
     6601 aaaacttaag gttctaaata gtttgtggtc gcgtagatac caatatttta attcttcata
     6661 catagagtaa aaatagttat ttttttaaaa caaaaagccc ttttcaacag aaagagcttt
     6721 tggtattcca ctttattttt tttgatttta aatcaatcct cttgaaataa caattctttg
     6781 aatttctgat gttccttcat aaatctgtgt gattttacta tcgcgcatca ttcgttctac
     6841 atgatattcg gctacatagc cgtttccacc atgaatttga acggcttcat ttgctacctc
     6901 aagtgcgatt tctgatgcat ataattttgc cattgcacca gaatgtgcaa tatcttttcc
     6961 ttcgtctttt tcgcaagctg cttttaacac taatagtttg gctgctgtaa cttttacaaa
     7021 catgtctgct agtttgaacg cgatggcttg gtgtttgaag atttcttttc caaatgcttt
     7081 tcgttcttga gaatatttta acgctaattc gtaagctcca gttgcaattc ctaatgcttg
     7141 tgatgcaata ccaattcttc cgccatttaa aacattcata gcaaatgaaa atccgaatcc
     7201 gtcggcaccg attctatttt cttttgggac ttttacatct gaaaacatta aagaatgtgt
     7261 atcgctacca cgaattccca ttttcttttc ttttggtcca atatcaaagc ctttccaacc
     7321 tttttctaca ataaaagcat tgattccttt gtgtcctttt tcaacatcgg tttgggcaat
     7381 tactaaatag gttgaggctg ttgctccgtt agttatccag tttttagttc cattcaataa
     7441 gtaatggtcg cctttatcga ttgctgtggt tttttgtgaa gttgcatcac ttccagcttc
     7501 tggttctgaa aggcaaaacg ctccaataac ttcacctttg gcaagtggaa cgaggtattt
     7561 cattttttgt tcttcgttgc aatatttttc cattccagca caaactaaag agttgtttac
     7621 tgacatgatt actgcggcag aagcatctac ttttgcaatc tcagtcattg ccaaaacata
     7681 agaaacacta tctaaacctg aaccaccata tttgggatca accatcattc caaggaaccc
     7741 tagttcaccc atcattttta cttgttctgt tgggaattta gagtgttcgt ctctttcaat
     7801 tacaccaggt aatagttctg cttgagcaaa atcttttgct gcttgctgaa tcattaattg
     7861 ttcttcggta agattaaaat ccatattttg tttattgtaa attgaatatt gtaaaatgta
     7921 tattgtattt ttgagtgcca aaagtacgtt ttaaaactta aaatttcaaa cgatttagta
     7981 attttagaat atgtctaagg aaagctataa agttgtaggt gtgatgagtg gaacttcgct
     8041 tgacggaatt gatttggctc atcttgattt tagtattgaa gatggaaaat ggtcgtttaa
     8101 agttcatgaa tgtgaaacaa ttgcttatcc taaagaatgg ttaaacaaac ttaaaatagc
     8161 ggtttctttt tcgaaagatg ctttagacga tttaaa
//
LOCUS       DAOOBH010000100         8082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_10791_length_8082_cov_5.443379, whole genome shotgun sequence.
ACCESSION   DAOOBH010000100 DAOOBH010000000
VERSION     DAOOBH010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10791_length_8082_cov_5.443379"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PK218_09380"
     CDS             complement(<1..162)
                     /locus_tag="PK218_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPW98761.1"
                     /translation="MSPIIKLKYFFEKHGFHVSSRLADKLGMRTSNVRIFFIYISFVT
                     AGLWFGVYLT"
     gene            complement(187..1455)
                     /locus_tag="PK218_09385"
     CDS             complement(187..1455)
                     /locus_tag="PK218_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPW98762.1"
                     /translation="MKEDFLHYVWQYKKFDFSNLKTVNGEPMHIINVGNYLQQTGPDF
                     FNGQILIDRQKWAGNIEIHIKSSDWYLHHHEKDNNYDNVILHVVWEYDTPIFRKDNSE
                     IPTLELKHHVAKEVLENYQSLLSPKSWIFCENQIASVDDFILKNWKERLFFERLERKT
                     MPIQKLLKILSYDWEAVLFCMLAKNFGLNTNGEVFLKMAQSIPFSVIRKERVDVENLE
                     ALFFDQTGLIPFEAEDNYTKELKKRVDYLTQKHQLQKTILAPVQFFKHRPDNFPTIRL
                     AQLAKVYHQQENLFSKIISLNKLTDFYSLFEVETGSYWQTHYQLDKESVKKKKKLSKS
                     FIDLIIINSIIPIQFAYAKSIGKEISETLLETMFEMQPERNTIIEKFNRFKIQSKNAF
                     DSQSLLQLKNEYCNANKCLQCSIGIQLLKS"
     gene            1514..3163
                     /locus_tag="PK218_09390"
     CDS             1514..3163
                     /locus_tag="PK218_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PNGase F N-terminal domain-containing protein"
                     /protein_id="HPW98763.1"
                     /translation="MKNFILISFLIFGKSFSQNYMITYSKSSNGKTIENQDVLMIFTN
                     ANHTLITTNNIISKKTEFPFEQNLVVKNSNEYIQIASLNDKKIIQTKDSISLNKQSFE
                     YLPDTKKILGYVCKKAKTTINSNTIELWYTNDLKLNAAPTILGQNLGLVLEMTRNGNY
                     SIIATKIEKTKSFPKLNLNSSESLDLVTYLDALLKSRFTTIKIFKDQIINFSEDSISN
                     DTILRFANGTIALRKMKFPVIKKGSQIFADITEQSNGDAYDRTGSFFIIPTLTSISFL
                     DGLKKGKAALPIYTNGNGKEYQGVVATQNYTPIVEMIRFFTPFGIKHYNTIQLKDKNW
                     QDNVYYRQDISDLRNLLNNQEVYVGIFIGNYDKGGHKVSANITIHTEEELQSKETKIL
                     PLFCTNNVMEMAGQEYGTMFNVDKGLEVKFKLDQTLKNCKLRYIVTGHGGWENGDEFV
                     PKKNSIYLDGKENFSFTPWRQDCGSYRLSNPASGNFSNGLSSSDYSRANWCPGTVTNP
                     IYIDLGNLEVGTHTIQVKIPQGLPEGNSFSSWNVSGVLIGE"
     gene            complement(3269..3385)
                     /locus_tag="PK218_09395"
                     /pseudo
     CDS             complement(3269..3385)
                     /locus_tag="PK218_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009089529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
     gene            complement(3387..4748)
                     /locus_tag="PK218_09400"
     CDS             complement(3387..4748)
                     /locus_tag="PK218_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPW98764.1"
                     /translation="MKYAENILGTIGNTPLVKLNKVVEGIDALVLAKVETFNPGNSVK
                     DRMALKMVEDAEADGRLKPGGTIIEGTSGNTGMGLALAAIVKGYKCIFVITDKQSKEK
                     MDILRAVGAKVVVCPTDVEPTDPRSYYSVSKRLSTEIPNSWYVNQYDNPSNAIAHYEQ
                     TGPEIWEQTDGKITHFVVGVGTGGTISGVAKYLKEKNPNIKIWGIDTYGSVFKKYHET
                     GIFDENEVYSYITEGIGEDILPKNVDFSLIDGFTKVTDKDAAVYTRKIALEEGIFVGN
                     SAGSCIKGLHQLKEHFMPEDVVVVLFHDSGSRYVGKMFNDDWMRERGFLEEEITKAED
                     LIKEHKDKPLVIVRTEELVSHAIDRLKKYNISQIPVMDINGFVGSIDETDLFRSYVEN
                     KNIADKPIREIMGKPYPIVKANTPIEEISKLITKDNQAVLVALENGKHHIITKHDVIS
                     SIK"
     gene            complement(4884..6842)
                     /locus_tag="PK218_09405"
     CDS             complement(4884..6842)
                     /locus_tag="PK218_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HPW98765.1"
                     /translation="MNRLIYNFILLVCFSFGFAQNAKKNLAPKDSKPTVINDGSKKSV
                     KANSPKATFDQYRIISIEKDTTYVDTSLTIQKEYKYNYLRKDIFGLLPFANEGQTYTV
                     LDYGLKTQNSFPEIGFSGKHFNYLEVNDINYYSVATPITELYFKTVMQQGQSLDAIIS
                     VNTSERFNFSIAYKGIRSLGRYINQLSSAGNFRFTSSYNTVNKRYYMNFHYTGQDLLN
                     GENGGLTTVSDFTSANPDFDNRERLAVYLRDAESFLKGKRFFVDHSFRINTNDVSNNL
                     YITHQFNFETKFFEYSQKTIASTIGTTKFNRFGDAYVFSDLKNQTHYNKMYNKVGAIY
                     ENKTLGKFQFFAEDFRYNYYYDRVLVLESGVIPNKLSQEINTVGGQYEYRKNKWNGVF
                     TASNSISKQPMSNLDAKLHYKLNEKNEFIFQYQLISKIPDVTYTLFQSDFVAYNWSNN
                     FKNEKINNFEATAKTQWLTASLQVSSLNDHLYFTNVSTIDNQQLISPKQYENTINYLS
                     IKVSREFKWWKLALDNTILYQKVDQQDPILNVPQIVTRNSLYFTNYFFKKALYLQTGF
                     TLNYFTSYYINDYNPVVAEAFVQNNTKVGDYPMLDFFINARIRQTRIFLKAEHFNSKM
                     SGNNFLTAPNYPYRDFTIRFGLVWTFFQ"
     gene            complement(6943..>8082)
                     /locus_tag="PK218_09410"
     CDS             complement(6943..>8082)
                     /locus_tag="PK218_09410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HPW98766.1"
                     /translation="DDFTIEQVGNKGGYDLLTGKSNSKNAIVVAAVYEVDNYIDATSV
                     EMSSFSNYGPTDDGRIKPDISAKGVGVSSCTSISNNSYDTYQGTSMATPAISGMILML
                     QKHYNNLNPSTFMRASTVRGLICHSAKEAGYEPGPDYEYGWGLADAEAAANIITNKNT
                     STILQENTLINNQVFTKLITLDTAQKLTATICWTDPTGSSNGAAEDNRTPRLRNNLDL
                     KILKNGSVYYPWKLDVEEPYTGATNFADNDVDNIEKVEIENAQPGVYTIQVSHKATLT
                     GGSQVFSLIANGSTGINLNTSNYDFDKNVFIYPNPTTNTLNYSINDAISIDSIRINDV
                     SGKAINNFKADLNHNNIDVSNLSSGIYFVTFVSEQSSVTKKFVKQ"
BASE COUNT         2726 a         1323 c         1171 g         2862 t
ORIGIN      
        1 ggttagatat accccaaacc aaagtccagc agttacaaaa gagatgtaaa taaaaaatat
       61 tctaacattt gaagttctca ttcccaattt gtctgccaag cgagaagaca catgaaatcc
      121 atgtttttca aaaaaatatt ttagctttat tatcggagac atcccttctt gttttgtggg
      181 ttaaaattaa gattttaata attgaattcc aatgctacat tgcaaacatt tatttgcgtt
      241 gcaatattca ttttttaatt gcaataaaga ttgcgaatcg aatgcatttt ttgattgaat
      301 tttaaaccta ttgaactttt caattattgt atttctttcg ggttgcattt caaacatggt
      361 ttccaatagt gtttctgaaa tttcttttcc tatactttta gcataggcaa attgaatcgg
      421 aattatagaa tttataataa tcaaatcgat aaaagatttc gataattttt tcttcttttt
      481 gacactttct ttatctagtt gataatgtgt ttgccaataa cttcctgttt caacttcaaa
      541 caatgaatag aaatcggtta atttgtttaa actaattatt tttgaaaaca aattttcttg
      601 ttgatgataa acctttgcca attgtgccaa tcgaatggta gggaagttat ctggtctgtg
      661 tttaaaaaac tgcacaggtg ctaaaatagt tttttgtaat tgatgttttt gagttaaata
      721 atcaactctt tttttcaatt ctttggtata attatcttcc gcttcaaatg gaatcaaacc
      781 agtttgatca aaaaacaatg cctctaagtt ctcaacatct acccgttctt ttctaatgac
      841 tgaaaatggt attgattgtg ccatttttaa aaaaacttct ccattagtgt ttaaaccaaa
      901 gttttttgcc aacatacaaa ataacacagc ttcccaatca tagcttaata tctttaacag
      961 tttctgaatt ggcattgttt ttcgttctaa ccgttcaaag aataaccttt ctttccaatt
     1021 ttttaggata aaatcatcaa ctgatgcaat ttgattttca caaaaaatcc atgattttgg
     1081 tgaaagcaat gattgataat tttctaaaac ttcttttgct acatgatgct tcaattcaag
     1141 agttggtatt tctgagttat cctttctaaa aattggtgtg tcgtattccc aaacaacatg
     1201 tagaattaca ttgtcataat tattgtcttt ttcgtgatga tgcaaatacc agtcggaaga
     1261 ttttatatga atttcaatat ttccagccca tttttgtcga tcaataagta tttgtccatt
     1321 aaaaaaatct ggacctgttt gttgtaaata attgccaaca tttattatgt gcattggttc
     1381 accattaacc gtttttaggt tagaaaaatc aaacttctta tattgccaca cgtaatgaag
     1441 aaaatcttct ttcatcaaaa aacaaattaa aacactaaag taagaaattt acttttacac
     1501 tcatccaaat actatgaaaa attttattct gatttcattc ttaattttcg gaaagtcatt
     1561 ttcacaaaat tatatgatta cttattccaa aagttcaaac ggaaaaacta tagaaaatca
     1621 agatgtttta atgattttta ctaatgcaaa ccatacttta attactacta ataacatcat
     1681 ttcaaaaaaa acagaatttc cttttgaaca aaatttagta gtcaaaaatt caaatgaata
     1741 tattcaaatt gcttctttaa atgataaaaa aatcattcaa acaaaagaca gcatttcact
     1801 aaacaaacaa agttttgaat atctacctga tactaaaaaa attcttggtt atgtatgcaa
     1861 aaaagcgaaa acaacaatca attcaaatac tattgaactt tggtacacta atgatttaaa
     1921 attaaatgct gctccaacca tcttagggca aaatttaggt ttggttttag aaatgactcg
     1981 taatggaaat tattctatta ttgcaactaa aattgaaaag acaaaatctt ttccaaaatt
     2041 aaacttaaat agctctgaaa gcttagattt agtaacttat cttgatgcat tgttgaaaag
     2101 tagatttact actattaaaa ttttcaaaga tcaaatcatt aatttttcgg aggattcaat
     2161 atcaaatgac accattcttc gttttgccaa cggaacgatt gcgcttagaa aaatgaaatt
     2221 tccagtcatt aaaaaaggaa gtcaaatttt tgcagacatc acagaacaat ctaatggaga
     2281 tgcttatgat agaacaggtt cttttttcat catcccaacc ctaacatcta tttcattttt
     2341 agacggtttg aaaaaaggaa aagcagcact gccaatttac actaatggaa atggaaaaga
     2401 atatcaaggt gttgtggcta cccaaaatta cactccaatt gttgaaatga ttcgtttctt
     2461 tacgcctttt ggaattaaac actacaatac gatacaacta aaagataaga actggcaaga
     2521 caatgtttat tatcgtcaag acatttctga tttgagaaat ctgttgaaca atcaagaagt
     2581 ttacgttggg atttttattg gtaattatga caaaggtgga cacaaagttt ctgcgaacat
     2641 cactatacat accgaagaag agttgcaaag caaagagaca aaaatacttc cgcttttttg
     2701 cactaataat gtaatggaaa tggccggtca agaatatgga acgatgttta atgtggataa
     2761 aggcttggaa gttaaattca aattagacca aactctgaag aactgtaaac tccgttatat
     2821 cgtaactggt cacggcggct gggaaaacgg agatgaattt gttccaaaga aaaactccat
     2881 ttatttagac ggaaaagaaa actttagctt tacaccgtgg cgacaagatt gcggttcata
     2941 tcgtttgtca aatccagctt cgggaaattt ttctaatgga ttgtcttctt cagattacag
     3001 tagagcaaac tggtgtccag gaacggtgac taacccaata tatatcgatt taggaaattt
     3061 ggaagttgga acacacacta ttcaagtgaa aattccacaa ggattgccag aagggaacag
     3121 cttcagctct tggaatgttt cgggagtttt gattggagaa taattatttt ttaatctcca
     3181 atgcattaat ggtaacaatt tttccttctt gcatgataac caaagattga tttttattgt
     3241 tttcttaaag agctcacgaa acttcgtttt catctgcact aacaaactct ttatattcta
     3301 acatttcagg taaaatggtg aaggaagttg ttgtttttcc tgcaccatta caacctgctg
     3361 tgatataaag atttatagct ttcactttat tttatcgaac taatcacatc atgcttagta
     3421 ataatgtgat gttttccatt ttctaaagct accaaaaccg cttgattgtc tttagtaatt
     3481 agcttggaaa tttcttcaat cggcgtattt gcttttacta ttgggtaagg tttacccatt
     3541 atttcacgaa taggtttgtc tgcaatgttt ttgttttcca cataacttct aaacaaatct
     3601 gtttcatcaa tacttcccac aaagccattt atatccatta ctgggatttg tgatatatta
     3661 tattttttta atctatcaat agcatgtgat actaattctt ctgttcttac tattactaat
     3721 ggtttgtctt tatgttcttt aattaagtct tctgctttgg taatttcttc ctccaaaaaa
     3781 cctctttcgc gcatccaatc atcgttgaac attttgccta catagcggct tccgctatcg
     3841 tgaaataaaa ccactacaac atcttcaggc atgaaatgtt cttttaattg atgcaatcct
     3901 ttgatgcaag aaccagccga attacccaca aaaattcctt cttccaaagc aatttttcga
     3961 gtataaacag cagcatcttt atcggttact ttagtaaaac catcaattaa agagaaatca
     4021 acattctttg gcaaaatatc ttcgccaatt ccttcggtaa tgtatgagta aacttcattt
     4081 tcgtcaaaaa ttccagtttc gtgatatttt ttaaaaacag aaccatacgt atcgattccc
     4141 caaattttaa tgtttgggtt tttctctttc aaatattttg caacacccga aatggttcca
     4201 ccagtgccta ctccaacaac aaagtgtgta attttgccat ctgtttgctc ccaaatttct
     4261 ggaccagttt gttcgtaatg tgctattgca ttggatggat tatcatattg attcacatac
     4321 caagaattag gaatttcggt acttaatctt tttgaaaccg agtaataaga acgaggatct
     4381 gtcggttcaa catcggttgg acaaacaact acttttgcac caacagcacg aagaatatcc
     4441 attttttctt ttgattgctt atcagtaatt acaaaaatac atttgtatcc tttaacaatc
     4501 gccgctaatg ccaaacccat tcctgtattt ccagaagttc cttcaatgat agttccacct
     4561 ggctttagtc ttccgtcagc ttcggcatct tctaccattt ttaaagccat tctatccttg
     4621 actgaatttc caggattaaa agtttctact ttggccaata ctaaagcatc aattccttca
     4681 acaactttgt taagctttac taaaggagta ttgcctatag tacctaatat gttttctgcg
     4741 tatttcattc tttttgtggt tactttttgt ggtgcaaatg tattgataat aaaaatgttt
     4801 acaaaaattc aattgaatta tcgtttgaat gtaaacttac aaagttataa cgctaaaatt
     4861 gttattattg aattttaaaa aaactattga aagaaagtcc aaaccaaccc aaaacggata
     4921 gtaaaatctc gataaggata atttggtgcg gtaaggaaat tgtttcctga catttttgaa
     4981 ttaaaatgtt ctgcttttaa gaaaatacga gtttgtcgga ttcgagcatt gatgaaaaaa
     5041 tcaagcattg gataatcacc tactttagta ttattttgca caaaagcctc cgcaacaaca
     5101 ggattatagt cattgatgta atagcttgtg aaatagttga gtgtaaaacc tgtttgcaaa
     5161 taaagtgctt tcttgaagaa ataatttgta aaatacaacg agtttcgagt aacaatttgt
     5221 ggtacgttta aaataggatc ttgttgatca actttttgat ataaaatggt attatcaaga
     5281 gctaatttcc accatttgaa ttcacgactg acttttattg ataaataatt tatggtgttt
     5341 tcatattgtt ttggagaaat gagttgttga ttatcaatag tgctaacatt ggtgaaatac
     5401 aaatggtcat ttaaagaaga aacctgtaaa gaggcggtta accattgcgt tttggctgtt
     5461 gcttcaaaat tattaatttt ttcgttttta aaattattcg accaattata agcaacaaaa
     5521 tcactttgaa aaagagtata agttacatcc ggtattttac tgattagttg gtattgaaaa
     5581 ataaattcgt ttttttcgtt taatttgtaa tgtagtttcg catcaagatt tgacataggt
     5641 tgctttgaaa tagagttaga agcggtgaaa acaccattcc atttattttt tctatattca
     5701 tattgacctc caacagtatt gatttcttga gaaagtttat ttggaatgac gccactttca
     5761 agaactaaca ctctatcata gtaataatta tatctaaaat cttcggcgaa aaattgaaac
     5821 ttacctaatg ttttgttttc atagatagca ccaactttat tgtacatctt attataatgc
     5881 gtttggttct ttaaatctga aaaaacataa gcatcaccaa atcgattaaa ctttgtagtt
     5941 ccaattgttg atgctattgt cttttgacta tattcaaaaa acttggtttc aaaattaaat
     6001 tgatgtgtga tatatagatt atttgaaaca tcatttgtat ttatacgaaa agaatgatca
     6061 acaaagaaac gtttcccttt taaaaaagat tcggcatctc tcaaatagac tgccaaacgc
     6121 tctctattgt caaaatcagg atttgcactg gtaaaatcgg aaacagtggt taaacctcca
     6181 ttttctccat ttaacaaatc ttgacctgtg taatgaaaat tcatataata gcgcttgttt
     6241 actgtattat agcttgatgt aaaacgaaaa tttccagcac ttgacaattg attaatatac
     6301 cttcccaaag accttattcc tttgtatgca atagaaaaat taaatctctc cgaagtattt
     6361 acagaaatta tagcatctaa cgactgtcct tgttgcatta cagttttaaa atacaattct
     6421 gtaattggtg ttgctaccga ataatagttg atatcattta cttctagata attaaagtgc
     6481 tttccagaaa aaccgatttc tggaaaacta ttttgagttt ttaatccata atctagcact
     6541 gtataggttt gtccttcgtt ggcaaaaggc aataaaccga atatatcttt tctaagataa
     6601 ttatacttat attctttttg aatggttaat gaagtatcaa cataagttgt atctttttct
     6661 atagaaatta ttcgatattg atcaaaagtt gcttttgggg aatttgcttt cactgacttt
     6721 ttggaaccat cattaatgac tgttggttta gagtcttttg gagctaaatt tttttttgca
     6781 ttttgagcaa acccaaaact aaaacaaacc agtaaaataa agttataaat taatctattc
     6841 attcttaaaa attacttagc aaaaatacac aaaactattt gtaataaaat cataaaaaaa
     6901 ccacctatga aggtggtttt aaaaatttaa tataaaaacg tcttattgtt ttacaaattt
     6961 cttagtaact gatgattgct cagaaacaaa agtaacgaaa taaattccag atgataagtt
     7021 tgaaacatca atattattat gattcaaatc agctttaaag ttatttatag ctttacccga
     7081 aacgtcatta attctaatag aatctattga tatagcatca ttaatagaat aattcaaagt
     7141 attagtagta ggattaggat aaataaatac atttttatca aaatcataat tactagtatt
     7201 caagtttatt ccagttgacc catttgcaat tagtgagaac acttgagaac cgcctgttaa
     7261 cgtggcttta tgagaaacct gaatagtata aacacctggt tgagcgtttt ctatttctac
     7321 tttttcaata ttatcaacat cattatcagc aaaattagta gcacctgtat atggttcttc
     7381 aacatccaac ttccatgggt aataaactga tccattttta agtattttta agtctaaatt
     7441 atttctaaga cgaggcgttc tgttatcttc agccgcacca ttagaacttc ctgttggatc
     7501 agtccaacaa attgttgcag tcagcttttg agctgtatct aaagtaatta gcttagtaaa
     7561 cacttgatta tttattagag tgttttcttg taaaatagta gatgtgtttt tatttgtaat
     7621 tatgtttgcc gcagcttctg catcagccaa accccaacca tattcataat ctggaccagg
     7681 ctcgtaacca gcttctttgg cagaatgaca aattaatcct ctaacagtcg aggctctcat
     7741 aaaagttgaa ggatttaaat tgttataatg cttttgcagc attaaaatca taccgctaat
     7801 ggcaggtgta gccattgaag taccctgata tgtatcgtaa gagttatttg aaatactagt
     7861 gcaagaacta acaccaacac ctttagcaga aatatcaggc ttgattcttc catcatctgt
     7921 tggaccgtaa ttactaaatg aactcatttc aacacttgta gcatcaatgt aattatcaac
     7981 ttcataaaca gctgcaacaa caatagcatt cttagaatta gattttcctg tcaataaatc
     8041 atacccacct ttattaccaa cttgttcaat tgtaaaatca tc
//
LOCUS       DAOOBH010000101         8070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_10811_length_8070_cov_5.214473, whole genome shotgun sequence.
ACCESSION   DAOOBH010000101 DAOOBH010000000
VERSION     DAOOBH010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10811_length_8070_cov_5.214473"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PK218_09415"
     CDS             complement(<1..1029)
                     /locus_tag="PK218_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98767.1"
                     /translation="MKKITYLVLFFLGIMTNSYAQLSEDFESGVFPPTGWTIETTNAT
                     NTWEETATGINGSSAYVSYDFLQDESLITPVFTVPAVNPKLFFNLQMSYYWAVDPNPN
                     YDLVVSVSTDGGSNWTQIWDETVLGVFPNFETINVSVDLAAYAGETNTQLKFEYIGDD
                     GADLLIDDVLVEAGPTTIPDCAANAVSTPDATCGNFATTLSWDAVAGVTGYTLTVGTT
                     TGGTDVLDAVNIGNVVSYVLPTQVAGISYFWTVIPVNDIGPATGCAENTYTTASTGCY
                     CTSEPVSFDNDGITNVTVGTTNFPTNPVSYFDHTATVADLTQGISNNIQISFATGYDY
                     NTYVLIDFN"
     gene            complement(1133..2317)
                     /locus_tag="PK218_09420"
     CDS             complement(1133..2317)
                     /locus_tag="PK218_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HPW98768.1"
                     /translation="MIIEPRMRGFICLTAHPDGCAKNVKNQIEYVKSKGKIDGAKKVL
                     VIGASTGFGLASRISSAFGCDAATIGVFFEKEPSEGKTATPGWYNTAAFETEATKAGL
                     YAKSINGDAFSDEIKQKTIDLIKKDLGQVDLVIYSLASPVRMHPKTGVLHRSSLKPIG
                     QKFTNKTVDFHTGTVSEVSIEPANQDDIDNTVVVMGGEDWTMWMNQMKEAGVLAEGAM
                     TVAYSYIGPEVTEAVYRKGTIGRAKDHLEATAFEITKDLASINGKAYVSVNKALVTQA
                     SSAIPVIPLYISLLYKTMKAKGIHEGCIEQMQRLYADRLYANTIALDENGRIRVDDWE
                     MRDDVQAEVSKLWEQATTDNLSEIGDLAGYKNDFLNLFGFGFEGVDYTAEANEMTLVP
                     SI"
     gene            complement(2450..4102)
                     /gene="recN"
                     /locus_tag="PK218_09425"
     CDS             complement(2450..4102)
                     /gene="recN"
                     /locus_tag="PK218_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPW98769.1"
                     /translation="MIASLSIENFALIEKLDINFSEGFSVITGETGAGKSILLGALGL
                     LMGKRADLSSLKNEQEKCIVEASFSIKKYNIQTLFQDNDVDYEDETIIRREILPSGKS
                     RAFINDTPVTLNVLQEISLHLIDIHSQHQNQELNDENFQIEILDVLANNMALVLEYQS
                     SLEDFNLAKGKLKQLVTQKNSASKEQDYNSFLLEELIAANLIAGEQEELEAELEKLSN
                     VEAIKENIEKSIAIATEEQIGVIQNLKEIKAAIAKIAALSNDYAVLFERISSTIIELD
                     DIISELNAEHDKLTHDPERLEFINQKLQLLYSLQKKHQVKTVEELITIKNDLDAQFSS
                     FEDIDNQIEQLTLQVEALKTKTDGIATTISSNRAGVVPSLEDQLIKILVPLGMPNARF
                     KFEINATETYFESGKDEIQLLFSANKGTNFGLLKKIASGGEMSRIMLAIKSILAQTKK
                     LPTIIFDEIDTGISGDVANKMADIMSKMSTDLQLFAITHLPQIASKGKNHYKVFKYIK
                     DENTFTEIKKLDKTERINEIAEMISGKNLTESAITHAKALLS"
     gene            complement(4126..5013)
                     /locus_tag="PK218_09430"
     CDS             complement(4126..5013)
                     /locus_tag="PK218_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPW98770.1"
                     /translation="MKKIIVFIFLCVTNFSNGQQLNCTIQINTDKVATTNVQLFKNMK
                     TSITDFVNKTDWTGETLKQHEKINCSMVIIINSFEDNNFSASIQVQSTRPIFNSTYSS
                     PIFNYNDNDFGFRYTEFENLIFNPSTFDSNLVSVLAYYSYMILGFDGDTYALNGGTKN
                     FEIAQQILTTAQQSGYKGWSQSDGTQNRFFLINDMLSGTFDSFRDTEYAYHRQGLDLM
                     TDDVKIAKENVMSAINLLAKLYSVRPNAFLTRVFFDAKADEIVSILSGGPSVTLATMV
                     DTLNRISPINSNKWATIKI"
     gene            complement(5067..6278)
                     /gene="coaBC"
                     /locus_tag="PK218_09435"
     CDS             complement(5067..6278)
                     /gene="coaBC"
                     /locus_tag="PK218_09435"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPW98771.1"
                     /translation="MSVLSGKKIVLGVSGGISAYKTATLVRLFIKAGAEIQVVMTPAS
                     KDFVTPLTLSTLSKNPVHSTFYNDEDENAQWNNHVELGLWADFMIIAPATANTLSKMA
                     TGNCDNILIATYLSAKCPVYFAPAMDLDMYKHKSTLHSFERLKEYGNIMIPAESGELA
                     SGLFGEGRMAEPENIISFIEMDIQSKLPLRGKKILITAGPTYEAIDPVRFIGNHSSGK
                     MGFDIANEAAKQGAEVILISGPTHQTLKYSSINLIKVISAEEMYQACQTYFGNVDVAI
                     CAAAVADYKPKVVADQKIKKSDAEFSIALEKTKDILASLGKEKKHQFLIGFALETENE
                     IENAKLKIQKKNLDLIVLNSLQDKGAGFGKPTNKVTFIDKNFKIEPKELKSKELVAED
                     ILEKVVLSFNN"
     gene            complement(6284..6604)
                     /locus_tag="PK218_09440"
     CDS             complement(6284..6604)
                     /locus_tag="PK218_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPW98772.1"
                     /translation="MDLKKTNAPVNTITYNKSVIEERTGNVYEAITIMAKRANQINSE
                     IKKELIEKLEEFATYNDSLEEVFENKEQIEVSKFYEKLPKPHALAVQEWLDDKIYYRD
                     TNQD"
     gene            complement(6607..7407)
                     /gene="bamD"
                     /locus_tag="PK218_09445"
     CDS             complement(6607..7407)
                     /gene="bamD"
                     /locus_tag="PK218_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPW98773.1"
                     /translation="MKKIFILFAFITLFSSCNQYQKALKSEDISVKFIEATKQYDAAK
                     YSKAIRLFEQIAPAYKGKPTAERLFYMYSQSLYKTKQYYLAGYQFESFAASYPKSEKV
                     EEASFLGAKCFSKLSPVYSLDQVDTYKAIDKLQSFIDKYPNSTYMNEANSVVKELREK
                     LEKKAFEIAKQYNTISDFKSAMVAFDNFILNYPGTPYKEKALFYKFDSAYQLAINSVP
                     AKMEERLLDAKKMYNNLIKFNNSSEFKDKADTMLARIDNDLKQFSKIN"
     gene            complement(7517..>8070)
                     /locus_tag="PK218_09450"
     CDS             complement(7517..>8070)
                     /locus_tag="PK218_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPW98774.1"
                     /translation="PTQEGIYQHYKAIAEVSPLPIILYNVPGRTASNVLPSTVIRLAN
                     DFKNIVAIKEAAGDIVQAMHLIHNKPKDFLVISGDDMITLPMILAGGSGVISVIAEGF
                     PKEFSEMVHLGLNRKVDDAYKIHYQLSKAIDMIFEQGNPAGIKEVFKHLGICENTVRL
                     PLVSVNDELSNRIKIFINNLYSA"
BASE COUNT         2493 a         1408 c         1286 g         2883 t
ORIGIN      
        1 attaaaatca attaaaacat atgtgttgta atcatatcct gttgcaaaag aaatttggat
       61 attattacta atcccttgtg ttaagtcagc tactgttgcg gtatgatcaa aatatgaaac
      121 aggatttgta gggaaatttg tagttcctac agtaacatta gtaattccat cgttatcaaa
      181 acttactggt tctgaagtac agtaacatcc tgttgaagct gtagtatatg tattctcagc
      241 acatccagtt gctggaccaa tatcgttaac aggaataaca gtccagaaat aagatatacc
      301 ggctacttgt gttggaagta cataagaaac tacatttcca atattaacag catcaagaac
      361 atctgttcct ccagtagttg ttccaactgt taaagtatat cctgtaacac cagcaactgc
      421 atcccaagaa agcgttgttg caaagttacc acaagtagca tctggagttg aaactgcgtt
      481 tgctgcacaa tctggaatag tagtcggtcc agcttcaact aaaacatcat caattaacaa
      541 atcagctcca tcatctccaa tgtattcaaa tttaagctga gtgtttgttt cacccgcata
      601 agcagctaaa tcaactgaaa cgttaatggt ttcgaagttt ggaaaaacac ctaaaacagt
      661 ttcatcccat atttgagtcc aatttgatcc accatctgta gagacagaaa caactaaatc
      721 ataatttgga tttggatcta cagcccaata ataactcatt tgtagattaa aaaaaagttt
      781 tggatttaca gcaggaactg taaaaactgg agttatcaat gattcatctt gtaaaaaatc
      841 ataagaaaca taagcacttg atccatttat accagtagca gtttcctccc aggtatttgt
      901 agcattggta gtttcaattg tccaaccagt tggtgggaaa actccactct cgaagtcttc
      961 ggacaattgc gcataactat ttgtcattat tccgagaaag aataagacta aataagtaat
     1021 ttttttcata atataaatta gtttaaaaag ttaatcctat aaaagtaaga taaaataaaa
     1081 aaactcgcaa gtaattaatg cgagtttttt ttagtcttta aatttaaaaa aattaaatac
     1141 ttggaactaa cgtcatttcg tttgcttctg ctgtatagtc aacaccttca aatccaaatc
     1201 caaatagatt taaaaagtcg tttttgtatc cggctaaatc tccaatttca gataaattat
     1261 cggtagtagc ttgttcccaa agtttagaca cttctgcttg cacatcatct ctcatctccc
     1321 aatcatcaac tctaatacgc ccgttttcat ccaaagcaat tgtatttgca tacaacctat
     1381 cggcatacaa tcgttgcatc tgctcaatgc atccttcgtg tattcctttt gctttcatgg
     1441 ttttatacaa caacgaaatg tataaaggta tcactggaat tgccgaactt gcttgggtta
     1501 ccaatgcttt atttaccgac acataagctt ttccattgat tgatgccaag tctttcgtga
     1561 tttcgaaagc tgttgcttca agatggtctt ttgcacgtcc gatggtacct tttcggtaaa
     1621 ccgcttcggt aacttctgga ccgatatacg aataagcaac ggtcattgct ccttctgcta
     1681 gcacgccagc ttctttcatt tggttcatcc acatagtcca gtcttcgcca cccatcacta
     1741 caacagtatt gtcaatatcg tcttgattag caggttcgat ggaaacttca gatacagttc
     1801 ctgtgtgaaa atctactgtc ttgttagtaa acttttgacc aattggtttc aacgaagaac
     1861 ggtgcaaaac accagttttt ggatgcatac gaactggaga tgctaagcta taaatgacta
     1921 agtcaacttg acctaagtct ttcttaatca aatcgatggt tttttgcttt atttcatcag
     1981 aaaaggcatc gccattgatg cttttggcat aaagacctgc tttagttgcc tctgtctcaa
     2041 aggcagcagt gttgtaccaa cccggagttg cggtctttcc ttcagatggt tctttttcga
     2101 agaagacacc tattgttgcg gcgtcgcatc caaatgcgct tgaaattcga gaggctaaac
     2161 caaaaccagt agaagctccg atgactaaaa ctttcttggc accgtctatc tttcctttag
     2221 atttaacgta ttcaatttga ttttttacat tcttagcaca tccatcagga tgagcagtca
     2281 aacaaataaa tccgcgcatt cttggttcta taatcataat gttgggtttt tatagttact
     2341 gtacaaaagt aatcaattta tatttttgtg tgtaaataaa aacgatacta gaggcttttt
     2401 tttgtttgat tttttaaaca aaaaaaactc aaatggctgt ttttagagac tagcttaata
     2461 aagctttggc atgagtgatt gccgattcgg ttaagttttt tccagaaatc atttctgcaa
     2521 tctcgtttat acgttcagtt ttgtcaagct ttttaatttc ggtaaaagtg ttttcgtcct
     2581 ttatatactt aaagactttg tagtggtttt tgcctttaga agcgatttgt ggcaagtgtg
     2641 ttatggcaaa aagttgcaaa tctgtactca ttttcgacat tatgtcggcc attttgttag
     2701 ctacatctcc agatattccg gtgtctattt cgtcaaatat aatggttggc aatttctttg
     2761 tttgtgccaa gatggatttt atagcaagca taattcggga catttctccg cccgaagcaa
     2821 tctttttgag aagaccaaag ttagtgcctt tattggccga aaataacagc tgtatttcat
     2881 ctttacccga ttcaaagtag gtttcggttg cgtttatttc aaacttaaac cgtgcgttgg
     2941 gcattcctaa aggtaccaaa attttgataa gttgatcttc taaactcggt actactcctg
     3001 ctctattgga tgaaatggtt gtagcaatac cgtctgtctt tgttttcaac gcctcaactt
     3061 gaagcgttag ttgctctatc tgattgtcga tatcttcaaa cgaactgaac tgagcgtcca
     3121 aatcattttt aatggtaatc aattcttcaa ccgtttttac ttgatgcttt ttttgcaacg
     3181 aatacaacag ttgcagtttt tgattgatga attccaaccg ttcaggatcg tgggttagtt
     3241 tatcgtgttc agcattcaat tccgaaataa tatcatcaag ttcaataatt gtgctcgaaa
     3301 ttctttcgaa caaaactgca taatcattag atagtgcagc aattttggca atagccgctt
     3361 taatttcttt taaattctga atgacaccta tttgttcttc ggttgcaata gcaatagatt
     3421 tttctatatt ttctttgata gcttcaacat tgcttagctt ctccaattcc gcttcaagtt
     3481 cttcttgttc gccagcaatt aagttggcag caattaattc ttctagaaga aatgagttgt
     3541 aatcttgttc cttggaagca ctattctttt gagttactag ctgcttcagt ttgcctttcg
     3601 caaggttgaa gtcttctagg ctggattgat attccaaaac caaagccata ttgttcgcta
     3661 atacatcaag gatttcaatt tggaagttct catcgttcaa ttcttggttt tgatgttgcg
     3721 aatggatgtc tattaaatga aggctaatct cttgtaaaac gtttagagta accggagtat
     3781 catttataaa agctctcgat tttcctgaag gaagaatttc tctccgaatt attgtttcgt
     3841 cttcataatc aacatcatta tcctgaaaaa gagtttgtat gttatatttt ttgatagaaa
     3901 atgaagcttc aacaatacat ttttcttgct catttttaag cgaagacaag tcagcgcgtt
     3961 ttcccattaa aagacctaaa gctccaagaa gtattgattt tccagcaccc gtttcacctg
     4021 taatcaccga aaagccttct gaaaaattaa tgtctaattt ttcaatcaaa gcaaagtttt
     4081 caattgaaag tgaagcaatc ataaaagtat ttgtaaaaaa aggaattaaa tcttaatagt
     4141 tgcccattta ttggaattga ttggcgaaat tctatttaaa gtatcaacca tagttgctaa
     4201 agtaacgctt ggtccaccag aaagtataga gacaatttca tcggctttgg catcaaaaaa
     4261 aacacgagtt agaaaggcat ttggtcttac cgaatataat tttgctaata aattaatagc
     4321 actcattaca ttttctttag caattttaac atcatcggtc attaggtcta aaccttgcct
     4381 gtgataggca tactctgtgt ctctaaaaga atcaaatgtt cctgataaca tgtcatttat
     4441 caagaaaaag cgattttgag taccgtctga ttgactccaa cctttataac cgctttgttg
     4501 tgcagtcgtt agtatttgtt gtgcaatttc aaagtttttt gtgccaccat ttaaagcata
     4561 agtgtcacca tcaaaaccta aaatcatata gctataataa gccaatactg atactaagtt
     4621 ggaatcgaag gttgaaggat tgaaaattag attttcaaat tcggtatatc taaacccaaa
     4681 gtcattgtca ttgtagttga atatgggtga tgaataagta gaattaaata ttggtcttgt
     4741 tgattgcacc tgtattgaag ccgaaaaatt attatcctca aaactattga tgataattac
     4801 catcgaacaa tttatttttt catgctgttt taatgtttca cctgtccaat cggttttatt
     4861 tacaaaatca gtaatggaag ttttcatgtt tttaaacaac tgaacatttg tagtagctac
     4921 tttatcagtg ttgatttgga tggtacaatt gagttgttgt ccatttgaaa aatttgtaac
     4981 acataaaaat ataaaaacaa taattttttt catgattcgg ttttatagta taacgaaaga
     5041 atggaattaa aagtataact tttgtttcaa ttattaaaac ttaagacaac tttttccaaa
     5101 atatcttctg caacaagttc ttttgatttc aattcttttg gctcaatttt gaaattttta
     5161 tctataaaag taactttatt agtgggtttt ccaaatcctg cgcctttatc ttgtaatgaa
     5221 tttaaaacta ttaaatccaa gtttttcttt tgaattttta atttagcatt ttcaatttca
     5281 ttttcggttt ctaaagcgaa acctattaaa aattgatgtt tcttttcttt tcctaatgat
     5341 gctaaaatat ccttagtttt ttcaagtgca atagaaaact cagcatccga ttttttaatc
     5401 ttttggtcag caacaacttt tggcttataa tcagcaactg ctgctgcaca aatagctaca
     5461 tcaacgttgc caaaataagt ttgacaagct tgatacattt cttcagctga aatgactttt
     5521 attaaattta tgcttgaata ttttaaggtt tgatgtgttg gtccagaaat tagaattact
     5581 tcagcacctt gtttggcagc ttcatttgca atatcaaaac ccattttccc agaagagtga
     5641 tttccaataa agcgaacagg atcaatagct tcatacgttg gtccagcggt aatcaagatt
     5701 tttttacctc gaagtggcaa tttactttga atatccattt caataaatga aataatattt
     5761 tcgggttctg ccattcttcc ttcgccaaaa agaccactgg ctaattcacc tgattctgct
     5821 ggaatcatga tgtttccata ctcctttaat ctctcaaaag agtgtagcgt tgacttgtgt
     5881 ttgtacatgt ccaaatccat agctggcgca aagtaaacag gacatttggc agaaagatag
     5941 gttgcaataa gtatattgtc acagttgcct gttgccattt tagaaagtgt attggctgtt
     6001 gctggtgcaa taatcataaa atctgcccaa agacctagct ctacatgatt attccattgg
     6061 gcattctcat cttcatcgtt gtaaaaagtt gaatgaacag gatttttgga aagagtagat
     6121 aatgttaacg gtgtaacaaa atctttagaa gcaggtgtca tgacaacttg tatctctgca
     6181 cctgctttta taaataatct tactaatgtt gctgttttat aggcagaaat accaccggaa
     6241 actccgagaa ctattttttt tccgcttaaa actgacattt tatttagtct tggttagtat
     6301 ctctgtaata gattttatca tctaaccatt cttgaacggc tagagcatga ggtttaggaa
     6361 gtttttcata aaacttagaa acttcaattt gttctttgtt ttcaaatact tcttctaaac
     6421 tatcattgta ggtagcaaat tcttccaatt tttcaattaa ttctttttta atttcagaat
     6481 taatttgatt ggctcttttt gccataatgg taattgcttc atacacattt cctgttcttt
     6541 cttcaattac acttttgttg taggtaattg tgtttacagg agcattcgtc ttttttaaat
     6601 ccatcactaa tttattttag aaaattgttt taaatcatta tctattcttg ccaacatcgt
     6661 atcggcttta tctttaaatt ccgaagaatt attaaattta atcaaattat tgtacatttt
     6721 ttttgcatcc aataatcgtt cttccatttt agcaggaaca ctgttaatag ctaattgata
     6781 tgccgaatca aacttataaa ataaagcttt ttctttgtaa ggtgttccag gataatttaa
     6841 aataaaatta tcaaaagcaa ccattgctga tttaaaatcg gaaatagtat tgtattgttt
     6901 tgcaatttca aatgcttttt tctctagttt ttcacgaagc tctttaacaa cactatttgc
     6961 ttcattcatg taggtagagt ttgggtattt atcaataaaa ctttgtaatt tatcaatagc
     7021 tttataggta tctacttggt ctaaactata aacaggtgac aattttgaaa aacattttgc
     7081 gccaagaaat gaagcctcct caaccttttc acttttagga taacttgcag caaaactttc
     7141 aaattggtat ccagctaaat aatattgttt tgttttgtat aatgattgtg aatacatata
     7201 aaataaacgc tcagctgttg gtttgccctt ataggctggt gcaatttgtt cgaataaacg
     7261 aatagcttta ctgtactttg cagcatcata ttgttttgta gcttcaataa atttaacgga
     7321 tatgtcttcc gattttaatg ctttttgata ctgattacaa gaagaaaaaa gcgtaataaa
     7381 agcaaaaagg ataaatattt ttttcattta ttgtttggtt actaatggtt ttattgactt
     7441 tgcacacttt taaaaccaac tgcaaattta ggtattaatt atggaataca aaatattttt
     7501 ttatgtacta aaaaatctaa gcagaataaa ggttgtttat aaatatttta atacgatttg
     7561 acaattcgtc attcacagaa accaatggca atcttaccgt gttttcgcaa attccgagat
     7621 gtttaaaaac ttctttaatt cccgctggat ttccttgttc aaaaatcatg tcgatagctt
     7681 ttgataattg ataatgaatt ttataagcat catctacttt tctgtttaaa cctaagtgaa
     7741 ccatttcgga aaattctttt ggaaaacctt cagctatcac agatataaca ccacttcctc
     7801 cagctaaaat catgggtaaa gttatcatat cgtctccaga aatcactaag aaatcttttg
     7861 gtttattgtg aatcaaatgc atggcttgta caatatcacc agcagcttct ttgatggcaa
     7921 caatattttt aaaatcattt gctaatcgaa ttaccgttga aggtaaaaca ttgcttgctg
     7981 ttcttcctgg aacattatac aaaataattg gcaaaggtga aacttcagca attgctttat
     8041 aatgttggta aattccttct tgagttggtt
//
LOCUS       DAOOBH010000102         8069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_10814_length_8069_cov_7.882081, whole genome shotgun sequence.
ACCESSION   DAOOBH010000102 DAOOBH010000000
VERSION     DAOOBH010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10814_length_8069_cov_7.882081"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..740
                     /gene="rplU"
                     /locus_tag="PK218_09455"
     CDS             96..740
                     /gene="rplU"
                     /locus_tag="PK218_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPW98775.1"
                     /translation="MYAIVEIAGQQFKVSKDQKVYVHRLASDEGSKVSFDKVLLLDDN
                     GNVTLGAPAIEGASVEAKVLQHLKGDKVIVFKKKRRKGYKKRNGHRQYLTQILVEGIS
                     ATGAKKAAPKAEKVEAPKAEKAPKASKKGDDLTLVEGVGPKAAEALVAAGVDTFAKLA
                     KTSAEDVKAILDASESKVQHLDPTTWAQQAQLAADGKMDELKKLQDELNGGRAV"
     gene            773..1033
                     /gene="rpmA"
                     /locus_tag="PK218_09460"
     CDS             773..1033
                     /gene="rpmA"
                     /locus_tag="PK218_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPW98776.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIYGGQAAIAGNIIVRQRGSK
                     HNPGENVYMGKDHTLHARVDGVVKFQKKGDDKSFVSVIPFEA"
     gene            complement(1259..2575)
                     /gene="ahcY"
                     /locus_tag="PK218_09465"
     CDS             complement(1259..2575)
                     /gene="ahcY"
                     /locus_tag="PK218_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPW98777.1"
                     /translation="MSTQTLPFVAYKVKDINLAAWGRKEIELAEAEMPGLMALRAEYG
                     ATQPLKGARIAGCLHMTIQTAVLIETLIALGAEVTWSSCNIFSTQDQAAAAIAAAGIQ
                     VYAWKGLDEESFDWCIEQTLFFGEDRKPLNMILDDGGDLTNMVFDRYPELIPGINGLS
                     EETTTGVHRLYERMKNGTLHMPAINVNDSVTKSKFDNKYGCKESAVDAVRRATDIMLA
                     GKRVVVCGYGDVGKGTAASFRGAGSIVTVTEIDPICALQAAMDGFEVKKLNTVVGNAD
                     IIITTTGNKDIVQGSHFEQMKDKTIVCNIGHFDNEIDMAWLNKNHGASKIEIKPQVDK
                     YTIKGKDIIILAEGRLVNLGCATGHPSFVMSNSFTNQTLAQIELWTNIENYKNEVYML
                     PKHLDEKVAALHLAKLGVELEELRPEQAEYIGVDVKGPFKPEYYRY"
     gene            2711..3340
                     /locus_tag="PK218_09470"
     CDS             2711..3340
                     /locus_tag="PK218_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantetheinyl transferase"
                     /protein_id="HPW98778.1"
                     /translation="MPVYQIFSIESHTKVYVWNISESFNELFRSVVLKDVSLARLEKM
                     KAESHQKGFLAVRKLLEVAGYDDFDLYYDDFGKPNLKDGKHISISHSNDFSVIAVSDC
                     NLGIDLEILKDKTLKLAPRYMDVSHLENLSKEEALEKATVIWGIKESIFKIKNIEGIS
                     FKNHIFEKKFELSVGKCSAELRFENQVEQFNIQHQKVDSYILVIAFRAN"
     gene            complement(3337..3726)
                     /locus_tag="PK218_09475"
     CDS             complement(3337..3726)
                     /locus_tag="PK218_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HPW98779.1"
                     /translation="MKREIQNRTDVSLLVHTFYDKIRANEEIGFFFNETIHDWDSHLE
                     KLTDFWENNLFAVRKYFGNPIEAHNKVDEKFNQSINPNIFGLWLNLWFETLDELFVGE
                     NVDILKRRARKMGTFLMVNIYENRKKT"
     gene            complement(3729..4016)
                     /locus_tag="PK218_09480"
     CDS             complement(3729..4016)
                     /locus_tag="PK218_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98780.1"
                     /translation="MILYNKSLQSFQKNYIGYSALVVIGQSCLGSAAAMYVLKNGVSL
                     IQIIQLGLVVTTCMLVNVSILSQMTPKFVFNFTIFSVLLSILLIALNTLVI"
     gene            complement(4089..4685)
                     /locus_tag="PK218_09485"
     CDS             complement(4089..4685)
                     /locus_tag="PK218_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPW98781.1"
                     /translation="MIANELLELYDAKRKKFQKGELIFAEGNEPHYYFQIITGEVKMC
                     NFNDVGREFIQGFFQDGQSFGEPPLFLNRAYPANAMAVTDCEIIIVPKNSFLELLKNN
                     NQVSLTIIEYLAQRLHYKSVMAAEISSQEPEHRLLKLIDYSIKFLKVQKGNDGFPIDL
                     TRQQMADLTGLRVETVIRAIKSLEKKKELKIIKRKVFR"
     gene            4785..5492
                     /locus_tag="PK218_09490"
     CDS             4785..5492
                     /locus_tag="PK218_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HPW98782.1"
                     /translation="MLYPQILVAKNQNKKQIAILLDPDKIEVNKLSSICDKINKTPAT
                     LVFIGGSSFEGKHLDLLIAELKAQIKLPIVLFPGNPAQISAEADAILFLMLLSGRNPE
                     YLIEHQVNAVPILAKTNLEIISTGYILIESGHQTAVERVSKTKPLSRTNLEYVAQTAK
                     AGEYIGNKLIYLEAGSGAKNPVPLEMIKKTSNTISIPLLVGGGIRSKKQIEEAFENGA
                     DMVVIGTAFENDLTFFD"
     gene            5492..6109
                     /gene="pnuC"
                     /locus_tag="PK218_09495"
     CDS             5492..6109
                     /gene="pnuC"
                     /locus_tag="PK218_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HPW98783.1"
                     /translation="MFDYLFAQYANYPTHEIILEITAVLFGLLSVWYAKKNNVLVFPT
                     GLISTMIYVYLLWKWSLLGDMIINGYYAIMSIYGWYHWTRKKEDVVEFPISITTKKEH
                     VWSWILFVAAMLFVIFVYNYFDKFTSWTAYIDTLTTGIFFAAMWLMAKRKIENWILWI
                     IGDIISIPLYFYKGYTFTSIQYIIFTIIAIYGYLEWKRILQQKNN"
     gene            6076..7677
                     /locus_tag="PK218_09500"
     CDS             6076..7677
                     /locus_tag="PK218_09500"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HPW98784.1"
                     /translation="MEENFTAKKQLKDNYGFTDNDFEQIKSHGIALDKIVEQLRIFED
                     GIPKIILDRPAKIDDGILKLSKEEFQKFAKYFDDQKPNLKLKKFVPASGAATRMFKFL
                     IEFLKEFDHQNESINAYINRKKDKNLPTFLAGIEKFPFYDEVKSRLKELHPNYYSLDS
                     HQKSYLFIKTMLEDQLFNFANKPKAALDFHNYQTYVAKPIEEHLNECACYASSNNASN
                     LHFTISEHHQQLFETIIDEVKPKIEKLTNTTTNISFSYQDKSTDTIAVGIENNPFRTE
                     NNQLLLRPGGHGALINNLNQLDADILFIKNIDNVIQNHIAEIALYKKGLAGKLLEIQK
                     QTHNYLKAIDANEITENQIPEIVAFMKHKLSIKLYEGFDKYTIENKIQSIKNKLNRPI
                     RVCGMVKNEGEPGGGPFWVRSPKGNLSLQIVETSQIDIENKLQAAILQSATHFNPVDL
                     VCSIKNYQGAKFDLTQFIDANTGFIVTKNRNGNDVKSYELPGLWNGAMAKWITVFVEV
                     PLITFNPVKTVNDLLKPAHQPQYEN"
     gene            7667..8068
                     /gene="arfB"
                     /locus_tag="PK218_09505"
     CDS             7667..8068
                     /gene="arfB"
                     /locus_tag="PK218_09505"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HPW98785.1"
                     /translation="MKTDILLSELSYKAIRSSGAGGQNVNKVASKVVLIFDAATTQAF
                     SEEELARIQTKLAKKISSENLLILTCDEDRSQRKNKQIVTKRFLELIEKALVVPKKRV
                     ATQVPKAVNEKRMKQKKSTAEIKQNRKKPDF"
BASE COUNT         2850 a         1390 c         1305 g         2524 t
ORIGIN      
        1 ttagtattat tggtaataat ttttctcaag atgttgtaca aaaaggaatt agttgtatat
       61 ttgcactctt aaaaaaatta attaaacaat ttgttatgta cgcaatcgta gagatagcag
      121 ggcaacaatt caaagtaagc aaagaccaaa aggtttatgt tcaccgttta gcatctgatg
      181 aaggttcaaa agtttctttt gacaaagtcc ttttattaga cgataatggt aacgtaactt
      241 taggcgcccc agctatagaa ggtgcttcag tagaagccaa agtgttacaa cacttaaaag
      301 gtgataaagt aatcgttttc aaaaagaaaa gaagaaaagg ttacaagaaa agaaatggtc
      361 acagacaata cttaacacaa attcttgtgg aaggtatatc tgcaactggt gctaagaaag
      421 cagctccaaa agcagaaaaa gttgaagctc caaaagctga gaaagctcca aaagcatcaa
      481 aaaaaggtga tgatttaaca ttagtggaag gtgttggtcc aaaagcagca gaagcattag
      541 tggctgcggg agttgatact ttcgctaaat tagctaaaac atctgctgaa gacgttaaag
      601 caattttaga tgcatctgaa tctaaagtac aacatttaga tccaacaaca tgggcacaac
      661 aagcacaact tgctgctgat ggaaaaatgg atgaattaaa aaaattacaa gatgaattaa
      721 acggcggtag agctgtttaa ttttagtatt aacttataaa aaccaaaaca tcatggctca
      781 caaaaaaggt gtcggtagtt cgaagaatgg tagagaatca gaatcaaaac gtttaggcgt
      841 taagatttat ggtggacaag ctgcaattgc tggaaatatc atcgtaagac aaagaggttc
      901 aaaacacaat ccaggagaaa acgtttacat gggtaaagat catactttgc atgcaagagt
      961 tgatggagtt gttaagtttc agaaaaaagg tgatgataaa tcatttgttt ctgtaattcc
     1021 ttttgaagca taagaattta aaaattcaaa tttaaaaacc caaattccaa aaagagtttg
     1081 ggttttttta tgtctttgac tttgcaatag tgataactgt ggtggctttt gcgaaacttt
     1141 gaaatatgga tttgaaaact ataacggata acgctctacc ttggcattag cccaaaaata
     1201 aaaccctcac ttttcagcaa gggttttagt cttaatactt aatactaaaa tcttaatact
     1261 aatacctgta atattctggt ttgaatggac ctttaacatc tacgccaata tattcagctt
     1321 gttccggtct aagttcttct aattcaacac caagtttcgc aagatgtaac gcagctactt
     1381 tttcatctaa atgttttggc aacatataaa cttcattttt atagttttct atgtttgtcc
     1441 ataattcaat ttgtgccaaa gtttggtttg taaacgaatt actcattaca aaacttggat
     1501 gacccgttgc gcaacctaag ttaaccaaac gaccttcagc caaaataata atatctttac
     1561 ctttaatagt atatttatca acttgtggtt tgatttcgat ttttgatgca ccgtggtttt
     1621 tgttcaacca tgccatatca atttcgttat caaagtgtcc aatgttacaa acaattgttt
     1681 tgtctttcat ttgctcaaag tgcgaacctt gaacaatatc tttatttccc gttgttgtaa
     1741 tgataatatc agcatttcca acaacagtat ttaatttttt aacttcaaaa ccgtccatgg
     1801 cagcctgtaa tgcacaaatt ggatcaattt cagtaacggt tacaattgaa ccagcaccac
     1861 ggaatgaagc tgcagttcct tttccaacat caccataacc gcaaacaacc actctttttc
     1921 ctgccaacat aatatctgta gcacgacgaa cagcatcaac agctgattct ttacaaccgt
     1981 atttgttgtc aaattttgat ttagtaaccg aatcgtttac gttaatagcc ggcatatgca
     2041 acgtaccatt tttcattctt tcatacaaac ggtgaactcc agtagtagtt tcttctgata
     2101 acccattaat tcctggtatt aactctgggt agcggtcaaa aaccatatta gttaaatcgc
     2161 caccatcatc caaaatcata tttaatggtt ttctgtcttc accaaagaat aaagtttgct
     2221 ctatacacca atcaaaagat tcttcatcta aacctttcca agcataaact tgaattcctg
     2281 cagcagcaat agcagcagct gcctggtctt gagtagaaaa aatgttacat gatgaccaag
     2341 taacttctgc tccaagagca atcaacgttt cgattaatac ggcagtttga atggtcatgt
     2401 gaagacatcc tgcaattctt gctcctttta atggttgtgt tgcgccatat tctgcacgaa
     2461 gtgccattaa acctggcatt tcagcttccg ctagttcaat ttcttttctt ccccaagctg
     2521 caaggttaat gtccttaact ttgtaagcta cgaatggtaa agtttgtgta ctcatctata
     2581 atttatttat attagctaaa aatttttgcg aatttacaaa ttaacctttc atcaaacaaa
     2641 gatttccaag atttttgtga tttgtttaat agtttaattt tctgaataac aattaaataa
     2701 tttcatttca atgccagttt atcaaatttt ttcaatagaa tcacatacaa aagtttatgt
     2761 ttggaacatt tccgaatcat ttaatgaact ttttcgttca gtggttttaa aagacgtttc
     2821 gttagctaga cttgaaaaaa tgaaggcaga aagtcatcaa aaagggtttt tagccgttcg
     2881 aaaattacta gaagttgcag gttatgatga ttttgatttg tattatgatg attttggaaa
     2941 gccaaatttg aaggatggaa aacacatttc aatttcacat tctaatgatt tttcggtgat
     3001 agccgtgagt gactgtaatt taggaattga tttagaaatt ttaaaagata aaaccttgaa
     3061 attagcacca cgatatatgg atgtttcgca tcttgaaaat ctttcaaaag aagaggcttt
     3121 agagaaagca acggttattt ggggtataaa ggaatctatt ttcaaaatta aaaatataga
     3181 aggaattagc ttcaaaaacc atatttttga aaagaaattc gaactttcag taggaaaatg
     3241 tagtgctgaa ctaagatttg aaaatcaagt tgaacaattt aatattcaac atcaaaaagt
     3301 tgatagttat attttagtta tagcgtttag agcaaactag gttttctttc tgttttcgta
     3361 aatattgacc ataagaaatg ttcccatttt gcgagcacgt ctttttagaa tatccacatt
     3421 ttctcccaca aatagctcgt ctaaagtctc aaaccaaaga tttaaccaca aaccaaaaat
     3481 gtttggatta atactttggt taaatttttc atcaacttta ttatgtgctt ctataggatt
     3541 tccaaaatat tttcgaacag caaacaagtt gttttcccaa aaatcggtta acttttctag
     3601 atgactatcc caatcgtgga tggtttcatt aaaaaaaaat ccaatttctt cattagctct
     3661 tattttatca taaaaagtat gaaccaacaa tgatacatcc gttcgatttt gaatttctct
     3721 tttcataatt aaatcaccaa agtgtttaaa gcaattagta aaatgctcaa aagcacacta
     3781 aatatagtaa aattaaaaac aaacttgggc gtcatttgcg aaagtatcga aacattgact
     3841 aacatgcagg tagtaacaac caatccaagt tgaataattt gtataagact tacgccattt
     3901 tttagaacat acatcgctgc agctgagcca agacaactct gcccaataac gaccaaagca
     3961 gaatatccaa tataattttt ctgaaaagat tgaagtgatt tgttgtaaag tatcataact
     4021 gtgtaatttt agtttacaaa gttactttgg ggtatttaca aatctttatg acttaaatca
     4081 caaatgggtt aacggaaaac tttccttttg atgattttta attccttttt tttctccaat
     4141 gatttgattg ctctaataac agtttccact cttaatccgg ttaaatctgc catttgctgt
     4201 cttgttaaat cgataggaaa accatcattt cctttttgaa cttttaagaa ttttattgaa
     4261 taatctatta gtttcaacaa tcgatgttcg ggttcttgtg aagaaatttc ggcggccatt
     4321 accgatttgt aatgcaatcg ttgtgctaaa tattcaataa tggttaacga aacttgattg
     4381 ttgtttttaa gtaactctaa aaaactattt tttggaacta ttattatttc acaatcggta
     4441 acagccattg cgttggcagg ataggctcta tttaaaaaca atggtggctc gccaaaactt
     4501 tgtccatctt gaaaaaatcc ttgaataaac tctctcccaa catcattaaa attacacatt
     4561 tttacttcac ctgtaataat ttgaaagtaa taatgcggct catttccttc tgcaaaaata
     4621 agttcgcctt tttgaaactt ttttcttttc gcatcatata gttctaaaag ctcatttgct
     4681 atcattgttt taaattcaac agtttcaaca aaattacaat tataatttta ggaattttaa
     4741 aaaagaacta catttacaat tctattaaat atagaagaaa aacaatgttg tatcctcaaa
     4801 ttttagtggc caaaaatcaa aataaaaagc aaattgctat tcttcttgat ccagataaga
     4861 ttgaagtaaa taaactgtca agtatctgtg ataaaatcaa caaaacacca gcaaccctag
     4921 tttttattgg tggaagttcg tttgaaggaa agcatttaga cctattaatc gccgaactta
     4981 aagcccaaat taaactacca attgtattat ttcctggtaa tcctgctcaa atttcggcag
     5041 aagctgatgc cattttattc ttaatgttac tttcaggaag aaaccccgag tatttaatcg
     5101 agcatcaggt aaacgcggtg ccaattttag caaaaaccaa tctagaaatc atctctactg
     5161 gttatatttt aattgaaagc ggccatcaaa ctgctgtcga acgtgtgagc aaaacaaaac
     5221 ctttgtcaag aacaaacctc gaatatgttg cccaaaccgc caaagctggc gaatatattg
     5281 gtaacaaact aatctatctc gaagcgggta gcggagcaaa aaacccagtt ccgctagaaa
     5341 tgattaaaaa aacttccaat actatctcca ttccgttgct tgttggagga ggaattcgct
     5401 ccaaaaaaca aatcgaggag gcctttgaaa atggagccga tatggttgtt atcggaacag
     5461 cttttgaaaa cgatttaacc tttttcgatt aatgtttgac tatttatttg cccaatatgc
     5521 caattatcca acgcatgaga ttatcttaga aatcacagcc gtgctttttg gattattaag
     5581 tgtttggtat gccaaaaaga ataatgtttt agtctttcca actggtttaa tcagcaccat
     5641 gatttacgtt tacttgttgt ggaagtggtc gttgcttgga gacatgatta taaacggata
     5701 ctatgccatt atgagtattt atggttggta tcattggaca cgcaagaaag aagatgtggt
     5761 tgaatttcct atttccataa caacaaaaaa agaacatgta tggtcctgga tactttttgt
     5821 tgccgcaatg cttttcgtga tttttgtcta taactatttt gataaattta catcttggac
     5881 ggcctatatt gacacgctaa ccactggaat tttcttcgca gcgatgtggt taatggcaaa
     5941 aaggaaaatc gaaaactgga ttttatggat tattggcgat atcatctcaa ttcctttgta
     6001 tttttacaaa ggatacacat ttactagcat tcaatacatt atttttacta ttattgccat
     6061 ttatggatat ttagaatgga agagaatttt acagcaaaaa aacaattaaa agataattac
     6121 ggatttacag acaatgactt tgaacaaatc aaaagtcacg gcattgcttt ggataaaatt
     6181 gttgaacaac taaggatatt tgaagacgga attcctaaaa taattctaga cagaccagcc
     6241 aaaattgatg atggaatttt aaaattatct aaagaagaat ttcaaaaatt tgccaagtat
     6301 tttgatgacc aaaaaccaaa tttaaaactc aaaaaatttg ttcctgcttc gggtgctgct
     6361 actcgaatgt ttaagttttt aattgaattt ttaaaagaat ttgaccatca aaacgaatcc
     6421 attaatgcct atattaatag aaaaaaagat aaaaatttac caacattctt agccggaatc
     6481 gaaaaatttc ctttttacga tgaagtaaaa agcagattaa aagaactgca tcctaattat
     6541 tattcgttag acagtcatca aaaaagctat ttgtttatca aaaccatgct tgaagaccag
     6601 ctgtttaatt ttgctaataa accaaaagca gcattagatt ttcataatta tcaaacctat
     6661 gtagcaaaac caattgaaga acatttaaac gaatgtgctt gctatgccag ttccaacaat
     6721 gcctccaatc ttcattttac gatttcagaa caccatcagc aattgtttga aacaataatc
     6781 gatgaagtga aaccaaaaat agagaaatta accaatacaa caaccaacat cagcttttcc
     6841 tatcaagata aaagcacgga tactatagct gttggtatag aaaacaatcc ttttcgcacc
     6901 gaaaataatc agttgctttt gcgtcctggc ggtcatggtg ctttgataaa taatctaaat
     6961 caacttgatg ccgatatact tttcatcaaa aacattgata atgttattca aaaccatatt
     7021 gctgaaattg ctttgtataa aaaagggtta gccggaaaat tattggaaat tcaaaagcaa
     7081 acccacaact atctaaaagc tattgatgcg aatgaaataa cagaaaatca aattcctgaa
     7141 atagttgctt ttatgaagca taaactcagt ataaaactct acgaaggatt tgataaatat
     7201 accattgaaa acaaaatcca atcaataaaa aacaaactaa accgtccaat acgagtttgc
     7261 ggaatggtaa aaaatgaagg agaacctggc ggcggaccat tttgggtgcg aagtccgaaa
     7321 ggaaatcttt cgttgcaaat tgtggaaact tcgcaaatag atattgaaaa caaacttcaa
     7381 gcagctattt tacaatctgc tactcatttt aaccccgtag atttggtgtg tagtataaaa
     7441 aattatcaag gtgctaaatt cgacttaacc cagtttatag atgctaatac aggctttatt
     7501 gtgaccaaaa accgtaatgg caacgacgtc aaatcatacg aactaccagg actgtggaac
     7561 ggtgctatgg caaaatggat taccgttttt gtagaagtgc ctctaattac gttcaaccct
     7621 gtaaaaacgg ttaacgattt gctaaaacca gctcaccaac cacaatatga aaactgatat
     7681 tttactttca gaattaagct ataaagccat ccgaagctcg ggtgctggcg gacaaaacgt
     7741 aaacaaagta gcgtctaaag tagtgctaat ctttgatgca gcaaccacgc aagcattttc
     7801 cgaagaagag ctagcccgaa tacaaaccaa actcgccaag aaaatttcgt ccgaaaacct
     7861 gttgatttta acctgcgacg aagaccgaag ccagcgcaaa aacaaacaaa ttgtaaccaa
     7921 acgttttttg gaactcatcg aaaaagcact agttgtgccc aaaaaaagag tggcaaccca
     7981 agtgccaaaa gccgtaaacg aaaaaagaat gaaacagaaa aaatccacgg cagaaataaa
     8041 acaaaaccga aagaaacccg atttttaaa
//
LOCUS       DAOOBH010000103         7975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_10945_length_7975_cov_5.387753, whole genome shotgun sequence.
ACCESSION   DAOOBH010000103 DAOOBH010000000
VERSION     DAOOBH010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10945_length_7975_cov_5.387753"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /gene="ribB"
                     /locus_tag="PK218_09510"
     CDS             complement(<1..940)
                     /gene="ribB"
                     /locus_tag="PK218_09510"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HPW98786.1"
                     /translation="MNYRKTIIMILNDTKIQLDTIEEAIEDIRQGKVIIVVDDEDREN
                     EGDFLAAAEKVTPEMINFMAKHGRGLICAPLTERRCKELELHSMVTNNTDHMETAFTV
                     SVDLKGNGVTTGISAADRAKTILALVDDTTKPFELARPGHIFPLTAKQGGVLRRTGHT
                     EAAIDFARLAGFKSAGVIVEIMNEDGSMARLPELYEVAKKFDLKLVSIEDLVAYRMQH
                     DSLIIKKEDFEIKTRFGEFRLRAYLQTTNKQVHIALTKGSWNNGDAILTRINSTQVNN
                     DILGTLTGDIDKKLDDMFSLISKEGKGAIVFINQEIQ"
     gene            complement(1356..2795)
                     /locus_tag="PK218_09515"
                     /pseudo
     CDS             complement(1356..2795)
                     /locus_tag="PK218_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
     gene            complement(2803..3444)
                     /locus_tag="PK218_09520"
     CDS             complement(2803..3444)
                     /locus_tag="PK218_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPW98787.1"
                     /translation="MKKVVSLILFLALSISMQGQDKKAKDLLDKVTAKIKSYDNIIID
                     FKYSLTNSKENINKESKGNVILDGNKYYLNFMGITKIYDGKKTYTIVPEDEEITISSL
                     DEKDENAITPSKMLTFFNTGFKYYWDIPQDVKGRKIQYIKLVPNSSKDARKEILLGID
                     SQTNIIYNVIEIGKNGTKTTLTVNSFKTNQPLSKNQFTFASSKYPNYYINKLD"
     gene            complement(3452..5887)
                     /locus_tag="PK218_09525"
     CDS             complement(3452..5887)
                     /locus_tag="PK218_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPW98788.1"
                     /translation="MAKTTKKETTTDKKNASTTKNRSKQYRVLFGFVLILFSIALLFS
                     FISFFIYGQEDQSAVTSLTDRDEIVKNWLGKFGAYLADFFIYRGFGAASFIFVKLFFL
                     SGAFFLLDLPIKKLKNSWFWDLFAIIILSITFGFFATTLPELGGTIGYEMNLFFQDYI
                     GKTGTILILVFGLLIYAIFKIKVSPEAIKTFFEKKKKDLTEDLKSIRTPSSSTDYNLE
                     EFAITEDEEEIAEPTLKTSTFEINKEALKPTIENASEINLEPTIKPTISTPTPSRELE
                     ITETTNEDFVIEKAEEEDVVEENLAAKLVADFGEFDPTLELSNYKFPTIDLLKEYKSV
                     GITINQEELEENKNRIVETLKNYKIEIAQIKATVGPSVTLYEIVPEAGIRISKIKSLE
                     DDIALSLSALGIRIIAPIPGKGTIGIEVPNKNPTMVPMRTVISATKFQEAEMELPIAL
                     GKTISNETFVVDLAKMPHLLMAGATGQGKSVGLNAVLTSLLYKKHPAEVKFVLVDPKK
                     VELTLFNKIERHYLAKLPDTEDAIITDNNKVINTLNSLCLEMDNRYNLLKDAMVRNIK
                     EYNDKFKSRKLNPENGHRFLPYIVLVVDEFADLIMTAGKEVETPIARLAQLARAIGIH
                     LIIATQRPSVNVITGMIKANFPARIAFRVTSKIDSRTILDSGGADQLIGRGDMLFSNG
                     NDLVRVQCAFVDTPEVEKIVEYIGSQKAYASAYLLPEYVGEDGSSTSLEIDISERDTL
                     FREAAEIIVTAQQGSASLLQRKLKLGYNRAGRLIDQLEAAGIVGPFEGSKARSVNITD
                     LTSLEQFLDNEQK"
     gene            complement(5933..6307)
                     /locus_tag="PK218_09530"
     CDS             complement(5933..6307)
                     /locus_tag="PK218_09530"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPW98789.1"
                     /translation="MEFKKDNSFFAGRIKSVRYTIIGAHKLITTEHSVMVQSSIAVLL
                     IFAGFYYDISREEWMMQILSTGLVLAIEGMNTAVEKICDFIHPEYHEKIGFIKDIAGG
                     AVLFAAIASIAVGLLIYVPKLM"
     gene            complement(6311..6808)
                     /gene="tpx"
                     /locus_tag="PK218_09535"
     CDS             complement(6311..6808)
                     /gene="tpx"
                     /locus_tag="PK218_09535"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPW98790.1"
                     /translation="MASITLGGNPINTNGELPKIGTKAPQFSMIKTDLSTVTLNDFAG
                     SKLVLNIFPSIDTGTCAASVRAFNTKASQLENTKVLCISRDLPFAQKRFCGAEGLENV
                     INLSDFNTGSFGKDYGLEILDSVLSGLHSRVVIVLDENGVVKYSEQVSEIADEPNYET
                     ALATL"
     gene            6930..7550
                     /locus_tag="PK218_09540"
     CDS             6930..7550
                     /locus_tag="PK218_09540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW98791.1"
                     /translation="MKVNYKEHWEKVFSTKKENEVSWYQPKPETSLNFFIDNTIPKQA
                     KIIDIGGGDSYLMDFLLELGYTNLYLLDISPKAIERIKNRLGAKAKQISFIISDVLDF
                     NSTETFDVWHDRASFHFQTSQENILNYKKKVTKYVKADGHVFIGTFSENGPKKCSGLD
                     ITQYNSKSLQTVFYPEFDLVSSFVENHKTPFDTNQNFIFAHFKKNE"
     gene            complement(7614..>7975)
                     /locus_tag="PK218_09545"
     CDS             complement(7614..>7975)
                     /locus_tag="PK218_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HPW98792.1"
                     /translation="NVTDYKNSNLENVLDFFHNDADMYKNIVDQLPLDDAIKYESGFS
                     LFKHKIPMWIFLISLLFFAVWSWKENLSLIPLLGLVSCLYMMAELSVWNWIYFTCWLL
                     IGLLIYFGFSYKNSKLN"
BASE COUNT         2647 a         1293 c         1147 g         2888 t
ORIGIN      
        1 attgaatttc ttgatttata aaaacaattg caccttttcc ttcttttgaa attaaagaaa
       61 acatatcatc aagtttttta tcaatatcac ccgttaaagt tcctaagata tcattgttaa
      121 cttgagtaga gtttattcga gtcaaaatgg catcaccatt attccaactt cctttagtaa
      181 gggctatatg aacttgttta ttcgtagttt gtaaataggc tcttagtcta aactccccaa
      241 aacgagtttt tatttcaaaa tcttcttttt taataatcaa actatcatgt tgcattctat
      301 aggcaaccaa atcttctatg gagaccaatt ttaaatcaaa ttttttggca acttcatata
      361 gttctggtaa tcgagccatt gagccatctt cattcattat ctcaacaatt actccagccg
      421 atttaaatcc agctaatcgc gcaaaatcaa ttgcagcttc agtgtgtcct gttcgtctta
      481 aaacaccacc ttgtttggcg gttaatggga aaatgtgtcc aggacgtgct aattcaaatg
      541 gtttggtggt atcatctact aatgctaata ttgttttggc tctatctgct gcagaaatac
      601 ccgttgtaac tccatttcct tttaaatcta cagaaactgt aaacgccgtt tccatatgat
      661 cagtattatt agtaaccatt gaatgtagct ctaattcttt acaacgtctt tcagttaacg
      721 gtgcacaaat taaccctctt ccatgctttg ccataaaatt tatcatttct ggcgttactt
      781 tttctgcagc ggctaaaaag tcaccctcat tttcacggtc ttcatcatca acaacaatga
      841 ttacttttcc ttgtcgaata tcttcgatgg cttcttcaat ggtgtcgagt tgtatttttg
      901 tatcatttaa aatcataatg attgtttttc tgtagttcat ttaaagcaat tctattataa
      961 atataaaaaa taaaatagaa aggtaatgca gctataagta gtatatatgt attaattggt
     1021 gttgtaatct cttttgatat aaaaatccgt ttcaagattt gctgagagta tgttaagcaa
     1081 gtaattagca atataaataa cagtgataga aggtaaatat ttttaagttc tataaataat
     1141 aataggagta aacctatatt tatgattaaa cctaaattac tataatattt gtattttttt
     1201 gaaagcttgt tgagttcggt tttttctaaa ttgaattttt tatcagttat taaaatcgtt
     1261 ttttcattta aagaattagt agaatcagca accggaactg cttctgggat agaattgcta
     1321 atttcagaaa ttaagattaa tttatttttc tttggaaata atttttcaaa ccattctaca
     1381 aatggaaata aattaattga catcattcct ttatcgtctg tcgctctttt ggttaaaaat
     1441 actgcaaatg gtaataatag taatgttgcc atccaacttc ccaaaaatgg tgtaatgcca
     1501 ttttcttgag ctaatttttt tccaaaagta ttgatgaaat gataaataat aaaaattaat
     1561 acggcaaaaa caatcggcaa tcccaaccca ccttttctga ttatagcacc cagtggagca
     1621 cctaaaaaaa acattaaaat acaggaaaat gcaattgcaa atttttcata aagtgcaatc
     1681 cagtgggcat taatgttttt tattttattt tcaaattcat agatagagtt gtctaacgaa
     1741 aaacttatac catctacagt tgcaatggca acttttaaaa tttgcagttt atttgtttta
     1801 tcaaaattgg ataaaattga attgtttact aatgaaattt tagtttttgt agaatcttga
     1861 gaattcatta ctgttcctat gccataactt gaacctgaat acgctgtacg atcatttata
     1921 ttatccacaa acgaatttaa atctttggag tagtttctag aaagtgaatc taatgtatat
     1981 tttaattcac ttaccgtaag catggtgtta gtgtttgtta tttcttcatt atcaccttct
     2041 gaggaattta attttgataa atcgatattg atgatgtatt tcttgaattc acttttggca
     2101 aaaggaactt tttttcgttc ttcatatttt tcaggcgtga tgtcttcgta ataatttccg
     2161 ttatttaata ctaattttaa aatattagat tctttgctac taactatttc accactttcg
     2221 gctttaatta ctgaattgtt tccagagcca aatctacctt ttttatgaat ggtaactttg
     2281 tctaaaaaac ggccttcatt tcctgatttt ttttcaactt ttatagtata actaccaaca
     2341 ttactaaatt gtccttcggc tatagccatt gctggtttta attgagcaat gtttcttcta
     2401 aaattcagaa atttatattc tgcataagga attacattgt tagcaaaaag aaaggctaca
     2461 atactcaata gtccaataaa aacaataaga ctgttcatgg cacgtttcaa cgaaatacca
     2521 gatgatttca ttgcagcaaa ttcatagttt tcagccaaac taccaaaagt catgatggaa
     2581 gccaaaagca cagaaagagg caaaaccaac ggaactactc taggtgttgc gaaaagtaaa
     2641 aactttataa cagaaataaa atctaaatct tttcctgcca attcagaaat aaataaccaa
     2701 accgtttgaa ggataaagat gaaaaatagt ataacaaata ccgttagaaa ggtagaagta
     2761 aataatttta ataagtactt gtcaagtatt ttcacttgtt ttttaatcta gtttattgat
     2821 gtagtaattt ggatatttac tggaggcaaa ggtaaactga ttttttgata agggttgatt
     2881 tgttttaaaa gaattaacag tcaaagtagt ttttgttcca ttttttccaa tttcaataac
     2941 gttgtaaatt atattggttt gggaatcaat acctagtaaa atttcttttc gtgcatcttt
     3001 ggaactattt ggtactaatt ttatatattg aatttttctt cctttaacat cttgaggaat
     3061 atcccaataa tacttaaaac cagtattgaa gaaagttaac atttttgaag gtgtaatggc
     3121 attttcgtct ttttcatcaa ggctcgatat ggttatttct tcatcttctg gaacaatggt
     3181 gtaggtcttt ttgccatcat atattttagt tattcccatg aaatttaggt agtatttgtt
     3241 accatctaaa attacatttc ctttactttc tttattaata ttttctttgg aatttgtaag
     3301 cgaatattta aaatcgatta tgatattgtc atacgattta atttttgcag ttactttatc
     3361 caataagtct tttgcttttt tatcttgtcc ttgcattgaa atactcaatg ctaaaaataa
     3421 aattaacgaa actacttttt tcattgttta attatttttg ttcattatct aaaaattgtt
     3481 caagtgaagt caagtctgta atgtttacac ttctagcttt acttccttca aacggaccaa
     3541 caatacctgc agcttctaat tggtctatta gcctaccagc acgattgtaa cccagtttca
     3601 actttctctg caataatgat gccgaacctt gctgtgccgt aacaattatt tcagcagctt
     3661 ctctaaataa agtgtctctt tcggaaatgt caatttctaa cgaggtacta cttccatctt
     3721 ctccaacgta ttctggaagc aaataggcac tagcataggc tttttgtgaa cctatatact
     3781 ctacaatttt ttctacctct ggtgtatcta caaaagcaca ttgtacacga accaaatcat
     3841 ttccgtttga aaacaacata tctccacgtc cgattaattg gtcagcaccg ccagaatcta
     3901 aaatagttct tgaatcaatt tttgaagtaa ctctaaaagc aattctcgct ggaaaattgg
     3961 ctttaatcat tccagtaata acatttacag atggtcgttg cgttgcgata attaaatgta
     4021 ttccaatagc acgagccaat tgtgccaaac gagcaattgg ggtttctact tctttaccag
     4081 cagtcatgat taaatcggca aattcatcta ctaccaatac gatataaggt agaaatcgat
     4141 gtccgttttc tggattcaat tttctagatt tgaacttgtc gttgtactct ttgatgttac
     4201 gaaccatagc atcttttagc aaattgtaac ggttatccat ttctaaacaa agtgaattca
     4261 aagtattgat tactttgtta ttgtcagtaa tgatagcatc ttctgtatca ggaagttttg
     4321 ctaaataatg acgttctatt ttattaaaca aggtaagttc aactttcttt ggatcaacca
     4381 aaacaaattt tacttctgct ggatgttttt tatataaaag tgatgtcaaa acagcattta
     4441 aaccaaccga ctttccttgt cctgttgcac cagccataag taagtgtggc atttttgcta
     4501 aatcgaccac aaaagtttca ttagaaattg ttttccccaa tgctattggc aattccatct
     4561 cagcttcttg aaattttgtt gcagaaataa ctgttcgcat cggaaccatt gttggatttt
     4621 tgtttggaac ttcaattcca attgtgcctt ttcctggaat tggtgcaata atacgaattc
     4681 ctaaagcaga taaggataga gcaatatcat cttctaaact tttaattttt gaaatacgaa
     4741 ttccagcttc aggaactatt tcatataatg taactgatgg tccaacagtt gctttgattt
     4801 gcgctatctc gattttgtag ttttttaaag tttctacaat tcggttttta ttttcttcta
     4861 attcttcttg gtttatagta attccaacag atttatattc ttttaataga tcaatcgttg
     4921 gaaatttata atttgacaat tctaaagttg gatcaaattc accaaaatca gcaactagtt
     4981 ttgccgctaa attttcttct acgacatctt cttcttcagc cttttcaatt acaaaatctt
     5041 cattggttgt ttctgtaatt tctaattctc tagaaggtgt tggagtagaa atggttggtt
     5101 taatggtagg ttctaagtta atctccgaag cattttcgat tgttggtttt aaagcttctt
     5161 tgttaatttc aaatgtggaa gtttttagtg ttggttccgc tatttcttct tcatcctcag
     5221 taattgcaaa ctcttctaag ttataatcgg tagaagacga aggtgttctg atacttttta
     5281 aatcttcggt taaatctttt ttcttttttt caaaaaaggt tttaatagct tctggagata
     5341 cttttatttt aaaaatagca taaattaaca atccaaagac taaaattaag atagttcccg
     5401 tttttccgat ataatcttga aaaaacaaat tcatttcata gccgattgtt ccaccaagtt
     5461 cgggcaaggt tgtagcgaaa aaaccaaaag ttatagaaag tataatgata gcaaacaaat
     5521 cccaaaacca tgagtttttt aattttttga tgggcaaatc caataaaaaa aatgcaccac
     5581 ttaaaaagaa cagttttaca aatataaatg aggcagcacc aaaaccacga tagatgaaaa
     5641 aatcagctaa ataggcacca aatttaccta accagttttt aacaatttcg tctctgtccg
     5701 ttaatgaagt aacggcactt tgatcttctt gaccatagat gaaaaaggaa atgaacgaaa
     5761 ataatagtgc gatagaaaaa agtatgagta caaaaccaaa taacactcgg tattgtttgc
     5821 ttcgattttt agtcgtcgaa gcattttttt tatcagtagt tgtttccttt tttgttgttt
     5881 tagccatagt tggtgtatcg ttgctataaa agtaaaaatt aaatctaaaa aattacatca
     5941 attttggaac atatatcaac aaaccaactg caattgatgc aatagcggca aataaaacgg
     6001 ctccaccagc aatatcttta ataaaaccaa ttttttcatg gtattctgga tgtataaaat
     6061 cacatatttt ttcaactgca gtattcattc cttcaattgc caaaaccaaa cctgtagata
     6121 atatttgcat catccattct tctcttgaaa tgtcataata aaaccctgca aaaattaaaa
     6181 gtactgcaat ggaagactga accatcacac tatgttctgt agtaatcaat ttgtgtgcgc
     6241 caataatggt atagcgaaca cttttgattc ttccagcaaa aaaagagttg tcttttttaa
     6301 attccataga ttataatgtt gctaaagctg tttcatagtt aggctcgtcg gcaatctcag
     6361 aaacttgctc agaatatttt acaacaccat tctcatctaa aacaataact actcttgaat
     6421 gcaatccaga caaaacactg tctaaaattt ctaaaccata atcttttcca aaactacccg
     6481 tgttaaagtc agaaagattg attacatttt ctaatccttc tgcgccgcag aaacgttttt
     6541 gagcaaaagg taaatctctt gaaatgcata aaactttagt gttttctaat tggcttgctt
     6601 tggtattgaa tgctctaact gaagcggcac atgtaccagt atcaatgctt ggaaaaatat
     6661 ttaacactaa cttacttcct gcaaaatcgt ttaaagtaac tgtgcttaaa tccgttttaa
     6721 tcattgagaa ttgaggtgct tttgtaccta tttttggtaa ttcaccattg gtattaatag
     6781 gatttccacc taaagttatt gatgccatat ttttttattt ttttaaatgt caaaagtaat
     6841 aaaaattaat tagctaataa agtcaattct tatctaactt gcagtgttgt taaagttaga
     6901 atttactaaa aatttaataa attcgcccaa tgaaagtaaa ttataaagaa cattgggaaa
     6961 aagtatttag cacaaaaaaa gaaaatgaag taagttggta ccaacctaag cctgaaactt
     7021 ctttaaattt ttttatagat aatacaattc ccaaacaagc taaaattatt gatattggtg
     7081 gtggcgacag ctatctaatg gattttttat tagaattggg ctatactaat ttgtatttat
     7141 tggatatttc tccaaaagct attgagcgta ttaagaatag gttaggagca aaggcaaaac
     7201 agatttcttt tataatttca gatgttttag attttaattc aacggaaact tttgatgttt
     7261 ggcatgatag agcttctttt cattttcaaa caagtcagga aaacatttta aattataaaa
     7321 agaaagtcac aaaatatgta aaagccgatg gtcatgtatt tattggaaca ttctctgaaa
     7381 atgggccaaa aaaatgcagt ggtttggaca taacacagta caattcaaaa tcgttacaaa
     7441 ctgttttcta tccagaattt gacttagttt ctagctttgt tgaaaaccat aaaactccat
     7501 ttgatactaa tcaaaatttt atattcgcac attttaagaa gaatgaataa atgatgtatc
     7561 taaaatattt attttttttg cgattaatct ttattaaata taggtgtttt tttttaattt
     7621 aatttgctat tcttgtagct aaagccaaag taaatcaata atccaattaa aagccaacaa
     7681 gtaaaatata tccagttcca aacactcagt tctgccatca tatacaaaca gctcactaac
     7741 ccaagaagcg gaataagaga aagattttct ttccatgacc aaaccgcaaa aaagagcaat
     7801 gaaataagga aaatccacat tggaattttg tgtttaaata aagagaatcc actttcatat
     7861 ttaatagcat catccagagg caattggtca acaatgtttt tatacatatc tgcatcatta
     7921 tgaaaaaagt ctaaaacatt ttctaaatta ctgtttttat aatcggttac atttt
//
LOCUS       DAOOBH010000104         7947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_11000_length_7947_cov_5.267739, whole genome shotgun sequence.
ACCESSION   DAOOBH010000104 DAOOBH010000000
VERSION     DAOOBH010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11000_length_7947_cov_5.267739"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="PK218_09550"
     CDS             complement(<1..1732)
                     /locus_tag="PK218_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98793.1"
                     /translation="MKNGLLLLLCFLFVNSLNSQNLLTNGDFENGANVGYITNGAGYN
                     ELTAPFSGTTVPGNYAVTNFPKTINTANFLFVGDKTSGNGKMLIIDGKSTTAGNTRFW
                     SAGNAGSGVTGLTIGTTYVFSYWIRSVSSLVTNAATQADISVLVTGGNLQTLVTGASL
                     APLPAVGWKQVAYTFVATATTVQIEMWNTKGSTVGNDFAIDEMMLTSDLMVTANVTNA
                     TCATANNGSMTVLGLLGVPPYINYSISGPVNQNNATGIFPNLPPGVYTVTVTDSALPT
                     ASTATLTNVVVGPQLTVSLDETICATVPITLNVGGSSSTYTWTANPADATLTTPNASS
                     ITVSPTVTTVYTVTSTVGSCAQMSKSVTVTVLPSTIPTFDPVAPICIGDTFAALPTTS
                     TNGIIGTWSPAIDNTITTTYTFTPTVPAQCLLNAQITLTVNQATVNPPIDQTICPGNV
                     ATVNLVGTAFSTVTLTNQDGATFVGNIGPAGTGTFVTPILQSTQVYTVVKVKNFFTQC
                     ERFYTGMSFTITVVPNGCATVGTVPAPGTQPLDLTLCSPGECRTIEANISDVPSTTSY
                     AASQIPYCPYP"
     gene            1889..2560
                     /gene="folE"
                     /locus_tag="PK218_09555"
     CDS             1889..2560
                     /gene="folE"
                     /locus_tag="PK218_09555"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPW98794.1"
                     /translation="MIQNREFEDEIGENHIATNATNPVRKDAFEISDDQKIELIKKDV
                     ENILQTLGMDLTDDSMKGTPNRVAKMFVKEIFGGLNPNKKPSSSTFENNYKYGEMLVE
                     KNITLYSTCEHHLLPIVGKAHIAYISSGQVVGLSKMNRIVDYFAKRPQVQERLTMQIV
                     QELQKVLNTKDVACVIDAKHLCVNSRGIRDIESSTVTSEFGGKFKEESTKREFLDYIK
                     LDTSF"
     gene            2696..4180
                     /gene="cysS"
                     /locus_tag="PK218_09560"
     CDS             2696..4180
                     /gene="cysS"
                     /locus_tag="PK218_09560"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPW98795.1"
                     /translation="MNALYKSQAVKLYNSLSGEKENFVPIDEGNIGMYVCGPTVYSNV
                     HLGNVRTFMSFDMIFRYFNHLGYNVRYVRNITDAGHLTDDGNVDNDRFVKQTRLEKLE
                     PMEVVQKYTVYFHRVLDLFNFLPPNIEPTATGHILEQIELTEKLIKDGFAYESNGSVY
                     FDVLAYNKKGLNYGELSNRNIDELLQNTRDLDGQNEKRNPQDFALWKKASPVHIMRWN
                     SPWSEGFPGWHLECTAMSTKYLGEHFDIHGGGMDLKFPHHECEIAQGKASTGKEPVKY
                     WMHTNMLTMNGQRMSKSTGNYILPMELINGENTFFEKAFNPNIVRFCFLQAHYRSVLD
                     ISNDAMIASEKGYIRLMEAVSNLNNIAPQNTSSIDIVSWKKSCYDAMNDDFNTPIVIA
                     QLFESVRFVNLLLDKKETISTIDLEDLKATLNVFLFDILGLKNETISANNSEKLSGVI
                     EMLIGMRNEARANKNFALSDQIRDNLMELGIQLKDGKEGTSFSL"
     gene            4180..4425
                     /gene="yidD"
                     /locus_tag="PK218_09565"
     CDS             4180..4425
                     /gene="yidD"
                     /locus_tag="PK218_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPW98796.1"
                     /translation="MIKKILIAPFLLLIRFYQLAISPFTPATCRFEPTCSHYYLEALK
                     IHGIFYGNYLGIKRILSCHPWGKSGYDPVPEKKSGSN"
     gene            4462..5373
                     /gene="lgt"
                     /locus_tag="PK218_09570"
     CDS             4462..5373
                     /gene="lgt"
                     /locus_tag="PK218_09570"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPW98797.1"
                     /translation="MIWNPTEGIDLGFFIIRYYSLMFVVAFGLGWYIMKHIYQREGES
                     LEKLDTLFIWTVVATLLGARLGHVFFYDWEYFRNHLSEILLPFKFDNGFEFTGFRGLA
                     SHGAAISIIIAIYFYSKKVIKRPMLWMLDRVVIPVASGAIFVRLGNFFNSEIIGKITD
                     EKFGIKFIRDFYSSADAVRLTKINNVNEAYNAIATKPEFAQLLAEVPYRYPAQLYEAI
                     CYVFVFLTLFFLYWKTNTAEKHGLLFGYFLVLLWSVRFIVEYVKESQGGIEHLFGLFS
                     TGQWLSIPFILVGIFYIFMAERPIKID"
     gene            complement(5374..6411)
                     /locus_tag="PK218_09575"
     CDS             complement(5374..6411)
                     /locus_tag="PK218_09575"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPW98798.1"
                     /translation="MNSNQDADFDEELYEHHKFVSPKGQSLLRVDKFLMQLIENATRN
                     KIQQAATDGNIWVNDIPVKSNYRVKPFDVVRVMLSHPPFENHIIPEDIPLDIVYEDDV
                     LLVINKPAGLVVHPGHGNYTGTLVNALAFHFDNLPMNSSERPGLVHRIDKNTSGLLVV
                     AKTEAAMTHLAKQFEDKSSEREYVALVWGNVKDDEGTIEGNIARHVKDRMQMAVFPDG
                     EIGKHAVTHYKVLERFGYVTLVSCKLETGRTHQIRVHMKYIGHTLFNDERYGGNLILK
                     GTTFTKYKQFIDNCFKTLPRQALHAKTLGFEHPTTKEFMSFNTDIPQDMAECIEKWRN
                     YSKSHTTDEDE"
     gene            complement(6416..7021)
                     /locus_tag="PK218_09580"
     CDS             complement(6416..7021)
                     /locus_tag="PK218_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPW98799.1"
                     /translation="MSFKNYITSKVFFTQVLIAFGIVALVAYAFFHWITYATNHGEEI
                     AVPNLAKLSAEQAGERLETLDLDYIILDTIDYNPDFPKLTVVDQEPKPGRKVKAGRKV
                     YLKINAEKFTMVVVPDLVEKTYRQALPTLNAVGLQEGSITYKPYLGKDMVLEMYSNGK
                     KLKAGDKVLKSSKIDLVLGDGNVVFDDSELDTLKVDTTDVK"
     gene            7165..>7947
                     /locus_tag="PK218_09585"
     CDS             7165..>7947
                     /locus_tag="PK218_09585"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HPW98800.1"
                     /translation="MKNIAIIMGGYSSEFEISLKSGNVVYQNLDPTKFKGYRIHVFKE
                     KWVYVDDLGFEFPIDKNDFSVIVKQTKITFDAVFNAIHGTPGEDGLMQAYFELLNLPQ
                     TSCGYYQAALTFNKRDLLSVLKPYGIKSATSYYLNQGDEINEDEILQKVGLPCFVKPN
                     KSGSSFGISKVKTKDELLIAIVNAYKEDDEIIIESFLDGTEVSVGVINYKGKVTVLPI
                     TEIVSENDFFDYEAKYLGQSQEITPARISEEVAAKVRNVAKKA"
BASE COUNT         2689 a         1280 c         1372 g         2606 t
ORIGIN      
        1 atggataagg acaatacggt atttgagaag ctgcataact cgttgttgaa ggcacatcag
       61 aaatatttgc ttcaattgtt ctacattctc caggtgaaca taatgttaag tctaagggtt
      121 gtgttcctgg tgcaggaaca gtacctactg tagcacaacc attaggtact acagttatcg
      181 taaaagacat acctgtataa aatcgttcac attgtgtaaa aaagtttttt actttaacaa
      241 cagtatatac ttgagttgac tgcaaaattg gagttacaaa tgttccagtt cctgctggtc
      301 caatgtttcc aacaaaagta gcaccgtctt gatttgtaag tgtaacagtt gaaaatgctg
      361 taccgactaa atttactgtt gccacatttc ctggacaaat agtttgatct ataggtggat
      421 taacagttgc ttgattaacg gtaagtgtaa tctgagcatt taaaagacac tgtgcaggaa
      481 cagttggtgt aaaagtatag gttgtggtta tagtgttatc tattgctggt gaccaagttc
      541 ctataattcc attcgttgaa gttgttggta atgccgcaaa agtatcacct atacagattg
      601 gagctacagg atcaaaagtt ggtatagtgc ttggcagaac cgtaacagtt actgattttg
      661 acatttgagc acaagatcca acagtggaag ttacagtata aactgtagta acagtgggag
      721 aaactgttat cgaacttgcg tttggtgtgg ttagagttgc atctgctgga tttgctgtcc
      781 aagtgtaagt actagagctt cctcctacat taagtgttat tggtacagtt gcacatatcg
      841 tttcatctaa actcacagtc aattgtggtc ctacaacaac atttgttaaa gtagcagttg
      901 aagctgttgg taaagcacta tcggtaacag taacagtata aacacctggt ggtaaatttg
      961 gaaaaatacc tgttgcatta ttttgattca caggtccaga aatactataa tttatataag
     1021 gtggtacccc taacaaacct aaaacagtca tggaaccgtt attagcagta gcacaagtag
     1081 cattagtaac attagccgtt accattaaat cactggtcag catcatttcg tcaatagcaa
     1141 aatcattacc aacagtactt cctttggtat tccacatttc tatttgcact gttgttgctg
     1201 tagctacaaa agtataagca acttgtttcc aacctacggc tggcaatggt gcaagcgaag
     1261 ctccagtgac caaggtttgt agatttccac cagtaaccaa aactgaaata tcagcttgtg
     1321 tagctgcatt ggttactaat gaagaaacag aacgaatcca ataactaaaa acataagttg
     1381 ttccaattgt taaacctgta acgccacttc cagcattacc tgcagaccaa aatcgtgtgt
     1441 taccagcagt ggtactttta ccatcgatga taagcatttt tccatttcca gaagttttat
     1501 caccaacaaa aagaaagttt gccgtgttaa ttgtttttgg aaaattagtt actgcataat
     1561 ttcctggaac tgttgtccca gaaaatggtg ccgtaagttc attgtaacca gctccattag
     1621 ttatataacc aacattggca ccgttctcaa aatcgccatt ggttaataaa ttctgtgaat
     1681 ttaagctgtt tacaaaaaga aagcataata atagtagtag tccatttttc atagcaagaa
     1741 aagtttagag gagcgaaatt tacataaatt taagaaaaaa ttatcataaa attttaattt
     1801 tttaaaactt tcgagtaatg attaattcat ttagtaatat tatttatctt tgctacctta
     1861 aaaaaaacta cacaaatatt gctattcaat gatacaaaac agagaatttg aagatgaaat
     1921 tggcgaaaat catattgcta caaatgctac aaatccagta agaaaagatg catttgaaat
     1981 ttcagacgat caaaaaattg aacttataaa aaaagatgtt gaaaacattc ttcaaacgtt
     2041 aggaatggat ttgacagacg acagtatgaa aggtactcct aacagagtgg ctaaaatgtt
     2101 tgtgaaagaa atttttgggg gattaaatcc gaataaaaaa ccaagttctt ctacatttga
     2161 aaataattac aaatatggag aaatgttggt tgaaaagaac attacactct actctacttg
     2221 tgaacatcat ttattgccaa tcgttggtaa ggctcatatt gcctatatct ctagcggtca
     2281 agttgttggt ttatcaaaaa tgaatcgaat tgtagattat tttgccaaaa gacctcaagt
     2341 tcaagaacgc ctaacgatgc aaattgttca ggaattacaa aaagttttga atacgaaaga
     2401 cgtagcttgt gtaattgacg ctaaacattt gtgtgttaac tctagaggaa ttcgagatat
     2461 agaaagcagt actgttactt cggaatttgg tggaaaattc aaagaagaaa gtaccaaacg
     2521 tgaattttta gattacatta aattagatac tagtttttaa tcatacttga tagatcaaaa
     2581 aaactaggga gacaatattt ttaatacttt gtcaccctga gcacagtcga agggcattaa
     2641 atcatatttt agaatgtgtt actccatata aaaagtagct caaaaaaaga aaaaaatgaa
     2701 tgcattatat aaaagtcaag ctgtaaaact atacaattca ctttcgggtg agaaagaaaa
     2761 ctttgttcca attgacgaag gtaacatcgg catgtatgtt tgtggaccaa cagtttacag
     2821 caatgttcac ctaggaaacg ttagaacatt catgtcgttt gatatgattt ttagatactt
     2881 caaccattta ggatataacg ttcgctatgt tagaaacatc accgatgctg ggcatctaac
     2941 cgatgatgga aatgttgata acgaccgttt tgttaaacaa acccgactag agaaattaga
     3001 acccatggaa gtggttcaaa aatatacagt ttattttcat cgagttttag atttattcaa
     3061 tttcttaccg ccaaatattg agccaactgc tacgggtcat attttggaac aaattgagct
     3121 aactgaaaag ttaattaaag acggctttgc ttatgaaagt aatggatcgg tttattttga
     3181 tgttttagcc tacaacaaaa aaggattaaa ttatggtgaa ttaagcaata gaaatattga
     3241 tgaattactt caaaatactc gtgatttaga cggacaaaat gaaaaacgaa acccacaaga
     3301 ttttgcactt tggaaaaaag catcacctgt acatatcatg cgttggaatt caccttggag
     3361 tgaaggtttt cctggttggc atttagaatg tactgccatg agcaccaaat atctaggaga
     3421 acattttgac attcacggtg gcggaatgga tttaaaattt ccgcatcacg aatgtgaaat
     3481 tgcacaagga aaagccagca ctggaaaaga accagtgaag tattggatgc ataccaatat
     3541 gcttactatg aacggacaac gcatgagtaa atcgactgga aattatattc ttcccatgga
     3601 attaatcaac ggtgaaaata ctttttttga aaaagctttt aatccaaata ttgttcgttt
     3661 ttgttttctg caagcgcatt acagaagtgt tttagatatt tctaatgatg ccatgatagc
     3721 tagcgaaaaa ggctatattc gtttaatgga agctgtttct aatttaaaca atattgcacc
     3781 acaaaacaca tcatcaattg acatcgtttc atggaaaaaa agctgttatg atgctatgaa
     3841 tgatgatttc aatacaccaa tagtaatcgc tcaactattt gaaagcgttc gttttgtgaa
     3901 tttactgtta gataaaaaag aaaccatttc cacaattgat ttagaagatt taaaagctac
     3961 tttgaatgtc ttcctatttg atattttagg attgaaaaac gaaactattt ctgccaataa
     4021 ttctgaaaaa ttatcgggtg ttatagaaat gttgattggg atgcgaaatg aagctagagc
     4081 caataaaaac tttgccttat ccgaccaaat tagagacaat cttatggaat tgggtattca
     4141 attaaaagat ggaaaagaag gaaccagttt tagtttataa tgataaaaaa gattttaata
     4201 gcaccctttc tattactaat ccgtttttat caattggcga tttcaccttt taccccagca
     4261 acatgccgat ttgagccaac atgttcgcat tattatctgg aagctctaaa aattcatggt
     4321 attttttatg gtaattatct tggaataaaa cgaattttga gttgtcatcc ttggggaaaa
     4381 tcaggttatg atccagttcc tgaaaaaaaa tcaggaagca attaataatt atccttattt
     4441 ttgaaaaata aatagaagaa aatgatttgg aatccaacag aaggaataga tttaggtttt
     4501 tttataattc gatattatag tttaatgttt gtagtagcct ttggtttagg ctggtacatc
     4561 atgaaacaca tctatcaacg agaaggagaa tcgttagaaa aattagatac tctatttatc
     4621 tggacggttg tagccacgct acttggagca cgactaggac atgttttctt ctacgattgg
     4681 gaatatttta gaaatcatct ttcagaaatt cttttacctt ttaaatttga caatgggttt
     4741 gagtttactg gatttagagg attggctagt catggtgctg ccatttcaat aatcattgct
     4801 atctattttt atagcaaaaa agttattaaa agacctatgc tttggatgtt ggacagagtt
     4861 gtgattcctg ttgcatctgg agctattttt gtgcgtttag gaaacttttt taattctgaa
     4921 atcattggaa aaattacaga tgaaaaattt ggaataaaat ttatccgaga cttctattct
     4981 agtgctgatg ctgttcgatt aacaaaaata aataatgtca atgaagcata caatgcaatt
     5041 gctacaaaac ctgaatttgc tcaactattg gctgaagttc cttatcgtta tcccgcacaa
     5101 ttgtatgaag caatttgtta tgtttttgta ttcttaaccc tattcttttt atattggaaa
     5161 acaaatactg ccgaaaaaca cggtttactt ttcggttatt ttttagtatt gctttggtct
     5221 gttaggttta ttgttgaata tgtaaaagaa agccaaggag gaatagaaca tttatttgga
     5281 cttttttcaa caggacaatg gctgagtatt ccgtttattt tagttggtat tttctatatt
     5341 ttcatggcag aaagacccat aaagatagac taattattca tcctcatccg ttgtatgtga
     5401 ttttgaataa tttcgccatt tttctataca ttctgccata tcttgaggta tatcagtatt
     5461 aaaagacatg aattctttag tagtaggatg ttcaaaacca agtgttttgg catgaagtgc
     5521 ttgtcttggc aaagttttaa aacaattatc tataaactgc ttgtattttg taaaagtagt
     5581 cccttttaaa atcaaattgc caccataacg ttcatcatta aacaaagtat gtccaatgta
     5641 tttcatgtga actctaattt ggtgtgttct tcctgtttcc aatttacaag aaactaaagt
     5701 tacataacca aaacgttcta aaaccttata atgggtaaca gcatgtttac ctatttctcc
     5761 gtcaggaaaa actgccattt gcattctatc tttcacgtga cgagcaatgt ttccttcaat
     5821 tgttccttcg tcgtctttaa catttcccca aaccaaagca acatattcac gctccgatga
     5881 tttatcttca aattgtttcg ccaaatgggt catggcggct tcggttttag caacaaccaa
     5941 aagtccagaa gtatttttat cgattcgatg cactaacccc ggtctttcac tagaattcat
     6001 cggaagatta tcaaaatgaa acgccaaagc attcactaaa gttcctgtat aatttccatg
     6061 tcctggatga acgaccaatc ctgctggctt gttaatcacc aacaaaacat catcttcata
     6121 tacaatatca agtggaatat cttctgggat aatgtgattt tcaaacggcg gatgcgaaag
     6181 cattacacgc actacatcaa aaggttttac tctataattt gatttaactg gaatatcgtt
     6241 tacccaaata ttaccgtctg tagccgcttg ttgaatttta tttcgggttg cattttcaat
     6301 taattgcatc aaaaatttat ctacacgtag taatgattgt cctttgggag aaacaaattt
     6361 atggtgttca tataactctt catcaaaatc ggcatcttga ttactattca tttcactatt
     6421 taacatctgt tgtatccact tttaaagtat ctaattccga atcgtcgaaa actacatttc
     6481 catcacctaa taccaaatcg atttttgatg atttcaatac tttatcgcca gctttaagtt
     6541 ttttaccgtt gctatacatt tctagtacca tatcttttcc taaataaggc ttataagtaa
     6601 ttgatccttc ttgtaaacca actgcattca gtgttggtaa agcttgtcga tacgttttct
     6661 caactaagtc gggtacaaca accattgtaa atttttcggc atttattttt aaatatactt
     6721 ttcgtccagc ttttactttt cgtccaggtt ttggttcttg gtcaactact gtaagttttg
     6781 gaaaatcagg attgtagtca attgtatcta aaataatata atctaaatct aaggtttcca
     6841 aacgttcgcc agcttgctca gcactcagtt ttgctaaatt tggtactgca atttcctctc
     6901 catgattggt agcataagta atccaatgaa aaaatgcata agcaaccaaa gctacaattc
     6961 cgaaagcaat taatacttgt gtaaaaaaga ctttactagt gatatagttt ttaaaactca
     7021 taaaaaattt aatttacgca aagatataaa aaacataaag cttattggtt tgaaaaaaaa
     7081 tgataatttt gtttaagcaa atttaattat caattttgaa gttatttgag caaagtaaaa
     7141 aatctactaa agaaaataaa atatatgaaa aacattgcca tcattatggg cggatattct
     7201 agcgaatttg aaatttcact aaaaagtgga aatgtggttt atcaaaatct tgacccaact
     7261 aaatttaaag gctatcgaat tcatgttttc aaagaaaaat gggtttatgt tgatgattta
     7321 ggttttgaat ttcctataga taaaaatgat ttttcggtaa ttgttaaaca aaccaaaatt
     7381 actttcgatg ctgtgtttaa cgccatacac ggaacaccag gcgaagatgg tttgatgcaa
     7441 gcttattttg aattattaaa tcttccgcaa acgtcttgtg gctattatca agcggcatta
     7501 acatttaaca aacgtgattt actttcggta ttgaaacctt atggcattaa gtcggctaca
     7561 tcctattatc ttaatcaagg tgatgaaatc aacgaagacg aaattttaca aaaagtaggc
     7621 ttgccttgtt ttgtaaaacc taataaatca ggttctagct ttggaatatc caaagtaaaa
     7681 accaaagatg aattgcttat tgccattgta aacgcttata aagaagacga tgaaattatt
     7741 attgaaagct tcttagatgg caccgaggtt tccgttggcg taattaatta caaaggaaaa
     7801 gttaccgttt tacctatcac agaaattgtt tctgaaaatg atttctttga ttatgaagcc
     7861 aaatatttgg gacaatcaca agaaataact ccagctcgaa tttcggaaga agtagctgca
     7921 aaagttagaa atgttgctaa aaaagct
//
LOCUS       DAOOBH010000105         7452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_11901_length_7452_cov_6.483980, whole genome shotgun sequence.
ACCESSION   DAOOBH010000105 DAOOBH010000000
VERSION     DAOOBH010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11901_length_7452_cov_6.483980"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PK218_09590"
     CDS             complement(<1..715)
                     /locus_tag="PK218_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW98801.1"
                     /translation="MEKELDEKKQGTSLVLEQKAENTKKLFIESYGCAMNFSDSEIVA
                     SILTEQGYNTTQNLEEADLVLVNTCSIRDKAEQTVRKRLEKYNAIKRSINPGMKVGVL
                     GCMAERLKDQFLEQEKIVDMVVGPDAYKDLPNLLKEVDEGRDAINVILSKEETYGDIA
                     PVRLNSNGVNAFVSITRGCDNMCTFCVVPFTRGRERSREPQSILEEIKDLWDRGFKEI
                     CLLGQNVDSYLWYGGGLKKD"
     gene            1352..3856
                     /gene="topA"
                     /locus_tag="PK218_09595"
     CDS             1352..3856
                     /gene="topA"
                     /locus_tag="PK218_09595"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPW98802.1"
                     /translation="MAKNLVIVESPAKAKTIEKFLGKDFQVESSYGHIADLPSKEIGV
                     DVLNGFKPKYEVSSDKKALVKKLKDLSKSAETVWLASDEDREGEAISWHLAEELKLDK
                     SKTKRIVFHEITKNAILKAIDNPREIDYNLVNAQQARRVLDRLVGYELSPVLWKKVKG
                     GLSAGRVQSVSVRLIVEREKEIQDFKPVASYSVSAEFTNADGKIVKAKLPKNFATKKE
                     AEDFLNKSIGSNYKVADLETKPTKKSPAAPFTTSTLQQEASRKLYLPVGITMQIAQRL
                     YEAGLITYMRTDSVNLSKEASEAAQAEIIRSYGKEYSKPRTFATKSKGAQEAHEAIRP
                     TDMSLHTVNIDRDQARLYDLIWKRTLASQMSDAELERTNVKIAADKHNELFNASGEVI
                     KFEGFLKVYLEGNDDEDEEQEGMLPALKVNEKLTNTVITAKERFTQAPSRYTEASLVK
                     KLEELGIGRPSTYAPTISTIIARTYVEKGSNEGSERKYNQLTLKNNEVKSQILSEMTG
                     SDKGKLVPTDIGTIVNDFLVKNFSAILDYNFTAKVEQDFDEIAEGNIDWAKMMQEFYD
                     KFHPNVKEVEENADRESGERILGTDPKSGKTILVRLGKFGPMAQIGEADDDEKQFASL
                     MPDQNISTISLEEALNLFLLPKQLGDYKGEEIEVNNGRFGPYVRFGKQFISLPKGEDP
                     MNITLERAQELINEKIKADAPIAAYKGKDVQKGVGRFGPFLKWNGIFINVSKKYNFDN
                     LTQSDINELIEDKIQKDIDKVIHNWESEGIKVEKARWGRSVITKGKIKIELSKDIDAA
                     AMTLEDVKELIEKKAPAKKTATKKKAAPKKATAKKK"
     gene            3860..5011
                     /locus_tag="PK218_09600"
     CDS             3860..5011
                     /locus_tag="PK218_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HPW98803.1"
                     /translation="MEFDFLSPVDSEIVQFVAKLSSQNLGNKVSFHTDKDFPDLDKIK
                     IALLGVPENRGDFNTYEEVNLHFIRKEFYKLYPGNWQSDIADLGDILPGNSVEDTFFA
                     LQKVVSVLLKKQIVPIILGGSQDLTYPLYRAYDKLEQMVNLVAVDSKFDIGKPDEEIS
                     SNSYLGKIIIDEPNNLFNYCNIGFQTYFNSQEEIDLIEKLYFDAYRLGEISNNITLAE
                     PVLRDADLVSIDLTTVKSSDSGNLYNFTPNGFNGKEICSLARYSGISDKVSCLGIFNH
                     NNTKEEAVLIAQMVWYFVEGVNFRSNEYPFTSKDNYSKYIVPLEEEEIIFYKSNKTER
                     WWIEIPFFSNLHNKLKKNTLLPCSQEEYLIACNQEIPERWWKAQRKNII"
     gene            5257..6663
                     /gene="gldK"
                     /locus_tag="PK218_09605"
     CDS             5257..6663
                     /gene="gldK"
                     /locus_tag="PK218_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldK"
                     /protein_id="HPW98804.1"
                     /translation="MKKFIAFASVLALLTSCGRSNDKGELVGVKGKKWNPEKPYGMTL
                     IPGGAFIMGKSDDDLANVQDAPTKTVTVRSFYMDETEITNSEYRQFVEWVKDSIIRVR
                     LAILADEVGQTAGSGGKGKSAGSIGDFAFNDSDPSKMTPYDKYMYENYYSVGTDDDPY
                     AGRRLNKKVKLITDTSKYPDEYYTEVMDTMYLPLAESFNGLRTIDVTKLKFRYSWMDI
                     QAAAKAKVGKRSSFIKTEQQQVYPDTTVWIKDFAYSYNEPMHNDYFWHQAYSEYPVVG
                     VSWKQAKAFCAWRTLNKNAYIKSKKKKQDLTNSFRLPTEAEWEYAARGGLESGTFPWG
                     GPYAKNDRGCFLANFKPNRGDYAADQALYTVEAKSYEPNGYNLYNMAGNVAEWTDSSY
                     DAAAYEYVSSMNPNVPDTKNMRKVVRGGSWKDVAYFLQVATRDFEYADTARSYIGFRT
                     VQDYMGTQVTGKAGAPRK"
     gene            6716..7345
                     /gene="gldL"
                     /locus_tag="PK218_09610"
     CDS             6716..7345
                     /gene="gldL"
                     /locus_tag="PK218_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HPW98805.1"
                     /translation="MALLSKKTMNFAYGMGAAVVIIGALFKLMHWPGASAMLIIGLGT
                     EALIFGLSAFDPVDTELDWSLVYPELAGGQAKAKDKKEDPKDAQGLLSQKLDNMLKEA
                     KIDGELMASLGNSIKNFEGAAKAISPTVDSVASTKKYAEEMTRAAEQLQTLNGLYQVQ
                     LQSAERNAKINEEVAENNLKLKDQMQSLTSNLSSLNNVYGGMLTAMSNR"
BASE COUNT         2702 a         1296 c         1310 g         2144 t
ORIGIN      
        1 aatctttctt cagtccgccg ccataccata aataactatc cacattttgc cctagtagac
       61 aaatctcctt aaatcctcta tcccacaaat ctttaatttc ttccaaaata ctctgcggtt
      121 cacggctgcg ttctcttcca cgagtaaacg gaaccacaca aaacgtacac atattatcac
      181 aaccacgcgt aatcgaaaca aatgcattca caccattact gttcaaacga accggtgcaa
      241 tgtcgccata ggtttcctct ttcgataaaa tcacgttaat cgcatctctg ccttcatcca
      301 cttccttcag caaattcggc aaatctttat acgcatccgg accaacaacc atatccacaa
      361 tcttctcttg ctccaaaaat tggtctttca atcgttctgc catacaaccc aaaacgccca
      421 ctttcatccc cggattaata ctgcgtttaa tagcgttata tttctccaaa cgttttctaa
      481 cggtttgctc agctttgtct ctaatcgagc atgtattcac caaaaccaaa tccgcttcct
      541 ccaggttttg cgtagtgtta taaccttgtt cggtcaaaat ggaagccaca atctcactgt
      601 ccgaaaaatt catcgcacag ccatagcttt ctataaaaag ttttttggtg ttttctgcct
      661 tttgttccaa aaccaagctc gtgccttgtt tcttctcgtc taattccttt tccataaaat
      721 aatttcgatt aaatcgggta gcaaagataa tactaaattt atttatatga caagatggca
      781 gacaaggttt aacaaagatt ttgataaata tttttaactg ttgttgtttt atttaaaccg
      841 ttttatcctt ggcaatccaa tttgtaactg ttataccatt cttacaatta acatctcctc
      901 ttttaattgt gttcatatta attttataat atgcagccac cattctagta acataatctt
      961 gagttatatc tgaacaatat ttttgtcttt cacaaaaatc caatatgttt tttatactta
     1021 cttctaatca ttatctgctt gatagatcaa gaatcctcta aatccgtgta atctgtggct
     1081 aataattcac ctcaatagtg agtcgcccaa gaataatttg tgcaaattgg tgtaatttgt
     1141 ggctaatcca tcccaataaa atttcaatta gtggaagtat atttttcgtc taacgaaaac
     1201 attttactag taaaacggtt ctcttgtgct catcgtgcct gctttgtacg catcattttt
     1261 atataaaatc caacctttta tcgtgaaaaa atagcaagta atcggatttt taaaaaaaac
     1321 tagtacattt gtccacacaa actacagaca aatggcaaag aatttagtaa tcgttgagtc
     1381 acccgcaaag gcaaaaacta tcgaaaaatt tctcggaaaa gacttccaag tcgaatcaag
     1441 ttatggtcac atagccgatt tgccttccaa agaaattggc gtagatgtat taaatggttt
     1501 caaaccaaaa tacgaagtct cgtctgataa aaaagcctta gtcaaaaaac tcaaagattt
     1561 atctaaatca gccgaaacag tgtggttagc ttccgatgaa gaccgcgaag gagaagccat
     1621 ttcgtggcat cttgcggaag aactaaaatt ggataagtct aaaacaaaac gtattgtttt
     1681 ccacgagatc accaagaacg cgattctaaa agccattgat aatcctcgcg agattgatta
     1741 taatttggtc aacgcacaac aggcacgacg tgtattagat aggctagtag gttacgaact
     1801 ttccccagtt ctgtggaaga aagtaaaagg tggtttgtct gccggtagag tacaatcggt
     1861 ttcagtgcgt ttaatagtag agcgcgaaaa agaaatccaa gactttaaac cagttgcatc
     1921 ctattccgtt tctgccgaat tcaccaatgc cgacggaaaa attgttaaag ccaaattacc
     1981 aaaaaacttt gccaccaaaa aagaagccga agattttcta aataaaagca tcggttccaa
     2041 ttataaagta gccgatttag aaacaaaacc aacaaaaaaa tcaccagcag caccgtttac
     2101 cacatcaacc ttacaacagg aagcctcacg taaactgtac ttacccgtag gcatcaccat
     2161 gcaaatcgca caacgcctct acgaagccgg attgattacc tatatgagaa ccgacagtgt
     2221 aaacctttcc aaagaagcgt cagaagctgc acaagccgaa atcattcgct cttacggaaa
     2281 agaatattcc aaacctagaa cctttgccac caaaagtaaa ggcgcacaag aagcacacga
     2341 agccatccgt cctaccgata tgtcattgca caccgtaaac atcgatagag accaagcacg
     2401 cttatacgat ttgatttgga aaagaacatt agcctcacaa atgagcgatg ccgaattaga
     2461 acgaaccaac gttaaaatag ctgccgataa gcacaacgaa ttatttaatg catcgggaga
     2521 agtaatcaaa ttcgaaggtt tcctaaaagt gtatcttgaa ggaaacgacg atgaagatga
     2581 agaacaagaa ggaatgttgc ccgctttgaa agtcaacgaa aagttaacca atactgttat
     2641 tactgccaaa gaacgcttta cacaagcacc aagccgctac acagaagcat ctttagtaaa
     2701 gaaattagaa gaattaggca ttggccgtcc ttctacttat gcaccaacca tatcaaccat
     2761 catcgcaaga acttatgtag aaaaaggttc caatgaaggt agcgaacgaa aatacaacca
     2821 acttactcta aagaataacg aagtaaaaag tcaaatcctt tcagaaatga caggttccga
     2881 taaagggaaa ttagttccaa ccgatattgg tactatcgtg aatgacttct tggtaaagaa
     2941 tttctcagcc attctagatt ataacttcac cgcaaaagta gaacaagatt tcgacgaaat
     3001 tgccgaagga aatattgatt gggcaaagat gatgcaggag ttctacgata agttccatcc
     3061 aaatgtaaaa gaagtagaag aaaatgccga tagagaaagc ggagaacgta ttttaggaac
     3121 tgatccaaaa tcaggaaaaa cgatattagt tcgtttagga aagtttggtc caatggcaca
     3181 aataggtgaa gctgatgatg acgaaaagca attcgctagt ttaatgcccg atcaaaatat
     3241 atccaccatt tctttagaag aagccttgaa tttattttta ttaccaaaac aattaggcga
     3301 ctataaggga gaagaaatag aagtaaacaa tggtcgtttt ggaccttatg ttcgcttcgg
     3361 aaaacaattt atatcactcc caaaaggcga agatccaatg aacataacct tggaacgagc
     3421 acaagaatta atcaacgaaa agattaaagc cgatgcgcca atagcagcat acaaaggtaa
     3481 ggatgtccaa aaaggagtag gtcgctttgg tcctttctta aaatggaatg gtatatttat
     3541 taatgttagc aaaaaataca actttgataa tttaacccaa tcagatatta acgaacttat
     3601 tgaagataaa attcaaaaag acattgataa agtcatccac aactgggaat ccgaaggaat
     3661 aaaagtagag aaagcacgat ggggaagatc tgttattact aaaggtaaaa tcaaaattga
     3721 attgagtaaa gatattgatg cagctgcaat gacacttgaa gatgtaaaag aattaatcga
     3781 aaagaaagca cccgctaaga aaacggcaac caaaaagaaa gccgcgccaa aaaaagcaac
     3841 cgctaaaaag aaataataca tggaattcga ttttttgtca cccgtagata gcgaaattgt
     3901 tcagtttgta gccaaactgt ccagtcaaaa cctaggcaat aaagttagtt ttcatacgga
     3961 taaggatttt cctgatttgg ataaaattaa aattgcccta ctaggagttc ctgaaaacag
     4021 aggcgatttt aatacttatg aagaagttaa tttacatttc attagaaaag aattttataa
     4081 actttatcca ggcaattggc aaagcgatat agctgattta ggagatattc ttcctggtaa
     4141 ttcagtagaa gatacttttt ttgcactaca aaaagtagtt tctgtattgt tgaagaaaca
     4201 aattgtccca ataatattag gtggaagcca agatttaacc tacccactat atcgtgctta
     4261 tgataaatta gaacaaatgg taaacttagt tgctgttgat tctaaatttg acataggaaa
     4321 accagatgaa gaaatatcat ctaattctta tttaggtaaa attataatcg atgaaccaaa
     4381 taatcttttc aactactgta atattgggtt tcaaacctat tttaattcac aagaagaaat
     4441 agatttaatc gaaaaactct atttcgacgc ctaccgattg ggtgaaatat cgaataacat
     4501 aacccttgca gagccagtat tgagagatgc agatttagtt tcaatagatt taacaaccgt
     4561 aaagtcatca gattcgggaa atttatataa ctttacaccc aatggattta atggaaaaga
     4621 aatatgtagc ctagctagat attcaggtat aagtgataaa gtgtcctgtt tgggtatttt
     4681 taatcacaac aatactaaag aagaagctgt cttaatcgct caaatggttt ggtattttgt
     4741 cgaaggtgtt aattttaggt caaatgaata tccatttaca tcaaaagata actattcaaa
     4801 gtatattgtt cctttagaag aagaagaaat catcttttat aagagcaata aaaccgagcg
     4861 ttggtggata gaaatacctt ttttttcaaa tttgcacaat aaattgaaaa aaaatacgtt
     4921 attaccttgt tcacaagaag aatatttaat agcgtgcaac caagaaatac ctgaaagatg
     4981 gtggaaagca caacgaaaaa atataatata gaaaatattt aagttacaaa attaataaaa
     5041 atctattttt aaatagaaaa aagattacaa aaacaacaat aaaacgcatt ttatcgatgt
     5101 ttttttgctt tttatcgata ataaattttt ttaattcaaa aattaatttt agttttgtcc
     5161 tgttaattaa tttttacatt aataattgtt tttttaaaaa atattaaata ggtttacgcc
     5221 cttaaaaaat tgagtattca aataacccca atttatatga agaagttcat cgcatttgca
     5281 tctgttttag ctttattaac aagctgtgga cgatctaacg acaaaggaga gttagtaggt
     5341 gttaaaggaa aaaaatggaa cccagagaaa ccatatggta tgactttgat acctggaggc
     5401 gcatttatca tgggtaaatc agatgatgat ttagccaacg tgcaggatgc tccaaccaaa
     5461 acagtaacag ttcgatcttt ttatatggat gaaactgaaa ttactaacag cgaataccgt
     5521 caatttgtcg aatgggttaa agactcaatt attagagttc gtttagcgat tcttgctgat
     5581 gaagttggtc aaacagctgg ttcaggagga aaaggaaaat cagcaggaag tattggcgac
     5641 tttgctttta atgattctga tccttcaaaa atgactccgt acgataaata tatgtatgag
     5701 aattattata gtgtaggaac tgatgatgat ccttatgcgg gtagaagatt aaacaaaaaa
     5761 gtaaaattaa tcacagatac tagtaaatat cctgatgagt attatacaga ggtgatggat
     5821 actatgtatc ttccattagc ggaatctttt aatggtttaa gaactattga tgttacaaaa
     5881 ttaaaattcc gttattcttg gatggatatt caagcagcgg caaaagccaa agtaggaaaa
     5941 agaagttctt ttattaaaac agagcagcaa caagtttatc cagatacaac tgtttggatt
     6001 aaggattttg cttattcata caatgaacca atgcataatg attatttttg gcatcaagca
     6061 tacagtgaat acccagttgt tggtgtttca tggaaacaag ccaaagcatt ttgtgcatgg
     6121 agaactttaa ataagaatgc atatatcaaa tctaaaaaga agaagcaaga cttgactaat
     6181 tcattccgtt tgccaactga agctgaatgg gagtacgctg ctagaggtgg attagagtca
     6241 ggtactttcc cttggggtgg tccttatgct aaaaatgata gaggttgttt cttagcaaac
     6301 tttaaaccaa atagaggaga ttatgctgcc gatcaagctt tatataccgt tgaagccaaa
     6361 tcgtatgagc caaatggata caatctttat aacatggcag gaaacgttgc ggaatggaca
     6421 gattcctctt atgatgctgc ggcttatgaa tatgtttcat caatgaatcc aaatgttcca
     6481 gacacaaaga acatgcgtaa agtggttcgt ggtggatctt ggaaagatgt tgcctatttc
     6541 ttgcaagttg ctactagaga ttttgaatat gcagataccg caagaagcta tattggtttc
     6601 agaactgttc aagattacat gggaacacaa gtgactggta aagctggcgc acctagaaaa
     6661 taatttttaa ccaactttaa atattaaaac ttaactaact aaaaaattta ttattatggc
     6721 attattaagc aaaaaaacta tgaatttcgc ttacggtatg ggagcggcag ttgtaatcat
     6781 tggagcatta ttcaaattga tgcactggcc aggagcaagt gcgatgctta ttattggatt
     6841 gggtacagaa gctctaattt ttggattgtc agcttttgat ccagtagata cagaattaga
     6901 ttggtcttta gtatatccag aattggccgg aggtcaagct aaagcaaaag acaaaaaaga
     6961 agatccaaaa gatgctcaag gactattgtc acaaaaatta gataacatgt tgaaagaagc
     7021 taaaattgac ggagagttga tggcaagttt aggaaacagt attaaaaact ttgaaggagc
     7081 agcaaaagct atttcaccaa ctgttgattc tgttgcttct acaaaaaaat atgctgaaga
     7141 aatgacaaga gctgcagagc aattacaaac attaaatggt ttataccaag tacaacttca
     7201 aagtgctgag cgaaacgcta aaatcaatga ggaagttgct gaaaataatt taaaattgaa
     7261 agatcaaatg caatcattaa catccaactt gtcttcattg aacaatgttt atggtggaat
     7321 gttaactgca atgagtaata gataattagt tttttaacta tatattttca aataaaataa
     7381 taatttaaac taattagaaa aaatggcagg aggaaaatta acccctagac agaagatgat
     7441 taacctgatg ta
//
LOCUS       DAOOBH010000106         7431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_11938_length_7431_cov_5.178010, whole genome shotgun sequence.
ACCESSION   DAOOBH010000106 DAOOBH010000000
VERSION     DAOOBH010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11938_length_7431_cov_5.178010"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PK218_09615"
     CDS             <1..421
                     /locus_tag="PK218_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HPW98806.1"
                     /translation="DAEKINLNNKLESTASIEKVNPADGKYTLAETKWKLLKLNDKIV
                     KQTNNKPNFIKMNSSDGMFSAFAGCNKMFGSYAMPSTNTISFSDIGATRMACPNMITE
                     DTFRQMLEMVTKYKLEKETLTFYGSAKTPIATFVAIK"
     gene            428..844
                     /gene="ruvX"
                     /locus_tag="PK218_09620"
     CDS             428..844
                     /gene="ruvX"
                     /locus_tag="PK218_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPW98807.1"
                     /translation="MPRILAIDYGFKRTGIAVTDELQIIASGLTTVPSNTAIAFLKDY
                     FSKEDVIKVLIGEPKQMNGQPSESTPIIEKFVADFKTAFPDMKIERVDERFTSKMAFQ
                     TMIDSGLKKKQRQNKGLVDEIAATILLQEFLTRKMI"
     gene            878..2353
                     /locus_tag="PK218_09625"
     CDS             878..2353
                     /locus_tag="PK218_09625"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HPW98808.1"
                     /translation="MEQPDVILIGAGIMSATLGVFLKELQPDCIIEIYERLDQPALES
                     SDAWNNAGTGHSAYCELNYTPLVDGKIDTKKAVHIAEQFQLSRQFWSYLIDNNKLPSP
                     DNFIHSVPHLSFVWGDENVAFLQKRYEALVKNPLFSTMQFSTDVDQLKAWMPLVMQDR
                     NEDKKFAATYMPLGTDVNFGALTRNLFAYLETLDGITIHYNHEVKKIKQKKDNTWQLK
                     VKDIVTGEKTKRKTKTVFIGAGGGSLLLLEKADIKEGEGYGGFPVSGQWLKCTNETII
                     AQHKVKVYGKATVGSPPMSVPHLDTRIINGKKELLFGPFAGFSTKFLKNGSYLDLIKS
                     LEFDNIIPMHSAGWHNLPLTKYLVEQVMQSDEERIEALKEYFPNAKLDDWQLLDAGQR
                     VQIIKKDKELGGILEFGTEVITTSDGSLACLLGASPGASTAVAIMLEIVPKCFAKEMQ
                     SEAWITKMKQIIPSFGYSLNDNPALCQAVIKKTSKTLNLEK"
     gene            2422..3009
                     /gene="def"
                     /locus_tag="PK218_09630"
     CDS             2422..3009
                     /gene="def"
                     /locus_tag="PK218_09630"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPW98809.1"
                     /translation="MIIPIYGYGEPVLRKVAMAVTPDYPNLKDIIANMYDTMYNAYGV
                     GLAAPQVGIAIRLFTIDTSPFSDDKDLSNEQQNQLKNFKKTFINAKILKEEGEEWSFN
                     EGCLSIPDVREDVFRKEQITIEYCEEDFVMKTEVYDGLIARVIQHEYDHIEGILFTDK
                     ISSLKKTLIKKKLQNIMDGKAFPDYRMKFANKKGR"
     gene            3051..3536
                     /locus_tag="PK218_09635"
     CDS             3051..3536
                     /locus_tag="PK218_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HPW98810.1"
                     /translation="MNVEKILAISGKPGLFTLKLQTRTGFVAESLIDGKKITVGLRSN
                     VSLLSEISIYTYSEEKPLVEIMRAIAIKENEGPTPVTTKDDPAKLAEYFTQILPEYDK
                     ERVYASDIKKLINWYNLLQSKGLVSKEEPKVENAETIKEQVVEQVKEEKTTKKATKKK
                     D"
     gene            complement(3598..>3854)
                     /locus_tag="PK218_09640"
     CDS             complement(3598..>3854)
                     /locus_tag="PK218_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98811.1"
                     /translation="AVGNEEIAVGNEEIAVGNGEIAVGSGEIAVGNRKIAIGSGEIAV
                     GNGEIAVGIEKVAVGSEEIAVGNGKIAVGSGEISRGKVSA"
     assembly_gap    3855..3954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3955..4166
                     /locus_tag="PK218_09645"
     CDS             <3955..4166
                     /locus_tag="PK218_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98812.1"
                     /translation="PNGNLFIPDGNLFIPDGNLFIPDGNLSTLDSNLFIPDGNLSTLD
                     GNFFIPDGNFSVPDGNRFNPNNKHF"
     gene            4310..7042
                     /locus_tag="PK218_09650"
     CDS             4310..7042
                     /locus_tag="PK218_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPW98813.1"
                     /translation="MALSWNEIKDRALRFSKEWADTTREEADAKPFLDAFFNVFGITR
                     KKIGTFEHKVKKISDADGYIDLLWKGTILIEMKSRGQNLDKAFQQAKDYTHGLKQNEL
                     PKYILVCDFDEFRLYDTEVQKTITFKLHELMDNVQHFGYIIGYQKKVYKEQDPANIKA
                     AELMGKLHDRLEEIGYVGHPLEVYLVRILFCLFAEDTTIFHKEQFADYIVQRTNEDGS
                     DLAAKIQELFQVLNTPVDKRFKNLDEQLNEFPYVNGKLFEEVLPTASFDSKMRQALLD
                     CCYIDWSKISPAIFGSMFQSVMNPKERRNLGAHYTSETNILKLIKPLFLDELWAEFES
                     IKTNKNKLAEFHKRISTLKFLDPACGCGNFLVITYRELRLLELEILRATYKSGQGVLD
                     VSQIIWLDVDMMAGIEYEEFPARIAEVAMWLIDHQMNLMISNEFGQYFARLPLKKAAK
                     IVHGNALRVDWEEVVSKKELSYIIGNPPFIGAAMKNNEQSAEMDFVFNKLSNNGKLDY
                     VAAWYLLAAKFINKTRIKAAFVSTNSVTQGEQVGILWNELIKEYNMCIHFAHRTFNWK
                     NDARGNAAVHCVIVGFANFEITNKYLFDYEDISGEATSYKVKNISPYLFDASNIIISK
                     TRIPVCKTPLMIRGNIPYDDGNLLLNEDEKNELINSDSNVSRYIKKYGGAYEMLNNAW
                     RYCLWFNDSFPPDLNKNSIIISRLKKCKEFREKSRSISVSSKSSTPYLFGDIRQPKSN
                     YILFPRVSSSKRKYLPIDYFESDIILADSGYGLPNANLYMFGVLHSIMHNSWLRNIGG
                     RLKSDYRYSNNIVYNNYPWPENPTEKQKQAIEDKAQKVLEVRASFPESSLADLYHPLT
                     MPPALVKAHNELDKAVDAAYSKQDFSSEAKRMEFLFELYEKYTAGLFVKEKKKK"
     gene            7048..>7431
                     /locus_tag="PK218_09655"
     CDS             7048..>7431
                     /locus_tag="PK218_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98814.1"
                     /translation="MTTIDTKYECVLDYLKRLKFQYESNYDKLMFFSIKPNDIIKYIT
                     NPEQCGNWPFEYVCKIEHSITQIFKKFIVDFPKIELQTEEDRVISKLLKKNPCFAFGT
                     NTFFDGTITVNQIIAYMEREEKIYQW"
BASE COUNT         2629 a         1312 c         1311 g         2079 t
ORIGIN      
        1 cgatgctgaa aaaataaatt taaataacaa actagaatca actgcttcaa tagaaaaagt
       61 aaatccagcc gatggaaaat atacgttagc cgaaaccaaa tggaagctgc ttaaattaaa
      121 tgataaaatt gttaaacaaa ctaataacaa acccaatttt atcaaaatga attcttccga
      181 tggaatgttt tctgcgtttg caggttgcaa caaaatgttt ggaagttatg ccatgccaag
      241 tacaaataca atatcttttt cggatattgg ggctactcga atggcttgtc ccaatatgat
      301 tactgaagat acctttaggc agatgcttga aatggttaca aaatataaac ttgaaaaaga
      361 aaccttaaca ttttatggtt ctgctaaaac accaattgct acctttgtag ctatcaaata
      421 agtacttatg ccaagaattc tcgccataga ttatggtttt aaaagaactg gaatagccgt
      481 tacagacgaa ttgcaaatca ttgcttcagg tttaactacc gtaccttcca acacggcaat
      541 tgctttctta aaagactatt tttcaaaaga agatgtcatt aaagtactca ttggcgaacc
      601 caaacagatg aacggacagc catcggaaag cacacctatt atagaaaagt ttgttgctga
      661 ttttaagaca gctttccccg atatgaaaat cgaacgcgtt gacgaacgat ttacttctaa
      721 aatggctttt caaactatga ttgatagcgg actaaaaaag aaacaacgcc aaaacaaagg
      781 tttggtcgat gaaattgccg caacaattct gctacaagaa ttcttaactc gcaaaatgat
      841 ataaattcat tttgaaattt tcattaataa caaaaacatg gaacaacctg atgtaattct
      901 tattggtgcc ggaataatga gtgccacttt gggtgttttt ctcaaagaac tacaacccga
      961 ttgtattatt gaaatctatg aacgcttaga ccaacccgca ctcgaaagtt ctgatgcctg
     1021 gaataatgcc ggaacaggac attcagccta ttgcgagtta aattacacac ctttagttga
     1081 tggaaaaata gacactaaga aagcagttca tattgccgaa caatttcaac tgtctcgcca
     1141 gttttggagt tatttaatag ataacaataa gctaccttct cccgataatt ttattcattc
     1201 agtgccacat cttagctttg tttggggcga tgaaaatgta gcctttctcc aaaaaagata
     1261 tgaagccttg gttaaaaatc ctcttttttc aacaatgcag tttagcaccg atgttgacca
     1321 actaaaagct tggatgccat tagtgatgca agatagaaat gaagacaaaa aatttgccgc
     1381 aacctacatg ccacttggaa cagatgtcaa ctttggtgca ttaactcgaa atctttttgc
     1441 ctatttagaa acgcttgatg gtataaccat tcactacaac cacgaagtaa aaaaaattaa
     1501 gcagaaaaaa gacaacactt ggcaattaaa agtaaaagat atagtcacag gtgaaaaaac
     1561 aaaacgaaaa actaagaccg ttttcattgg tgcaggtggc ggttcactat tacttcttga
     1621 aaaagcagac ataaaagaag gcgaaggcta tggcggattt cctgtctccg gtcagtggtt
     1681 aaaatgtacc aacgaaacca tcatcgccca gcataaagta aaagtatatg gaaaagcaac
     1741 tgtaggatca ccaccaatgt cagtgcccca tttagacacc cgaattatca acggaaagaa
     1801 agaattgctt ttcggaccat ttgcgggctt ctccaccaaa tttttaaaga acggttccta
     1861 tctcgatttg attaaatcat tagaatttga caacatcatc ccaatgcatt ctgcaggttg
     1921 gcacaatctt ccattaacaa aatatctggt agaacaagta atgcaatccg atgaagaacg
     1981 aatcgaagca ctcaaagaat atttcccaaa cgcaaaactt gacgactggc aactattaga
     2041 tgccggtcaa cgcgtacaaa tcatcaagaa agacaaagaa cttggcggca tactagagtt
     2101 tggtaccgaa gtaatcacta catccgatgg ctcattggcg tgtctattag gcgcatcgcc
     2161 gggtgcttct acagcagtag ccattatgtt agagatagta ccaaaatgtt ttgccaaaga
     2221 aatgcaatca gaagcatgga ttaccaaaat gaaacaaatc attccatcat ttggttactc
     2281 attaaatgat aacccagcat tatgccaagc agttattaag aagacaagca aaaccttaaa
     2341 tttagagaag tagatttgaa tttttgttta cttttgcact tttaataaga aatctaaaat
     2401 ctaatatcgt aattcaagaa tatgattatc ccaatatacg gctacggtga acctgtttta
     2461 agaaaagtag caatggcagt tacacccgac tatcccaacc taaaagacat cattgcaaac
     2521 atgtacgata caatgtataa cgcctatgga gtcggactag ctgcgccaca agtaggcata
     2581 gccattcgtt tgtttactat cgatacttct ccttttagtg acgacaaaga tttatccaat
     2641 gaacaacaaa accaactaaa aaacttcaaa aaaacattta ttaatgcaaa aattctaaaa
     2701 gaagaaggcg aagaatggag ttttaacgaa ggatgtttga gcattccaga tgttcgtgaa
     2761 gacgttttcc gaaaagaaca aattacgata gaatattgcg aagaagactt tgtgatgaaa
     2821 accgaagtct atgatggatt aattgctcga gttattcaac acgaatacga ccacattgaa
     2881 ggaatattgt ttaccgataa aatctcatcg ctaaaaaaaa cattaatcaa aaagaaattg
     2941 caaaacatca tggacggaaa agcttttcca gactatcgca tgaaatttgc taacaaaaaa
     3001 ggaagataat ttttgaaata ctatatattt gcaaccaata aaaaaataag atgaacgtag
     3061 agaaaatact agccatttca ggtaaacctg gtctatttac tttaaaatta caaacccgaa
     3121 caggattcgt tgcagaatca ttaatagacg gaaaaaaaat taccgtagga ttgcgtagca
     3181 acgtaagcct attgagtgaa atttccattt acacctatag cgaagaaaaa cctttagtcg
     3241 aaatcatgcg tgccatagcc atcaaagaaa acgaaggacc aacaccagta actaccaaag
     3301 acgatcccgc taaactagca gaatacttta cccaaatcct tccagaatac gacaaagaaa
     3361 gagtatatgc gtccgatata aaaaaactaa tcaattggta caacctgctt caaagcaaag
     3421 gattagtttc caaagaagaa cccaaagtag aaaacgcaga aacaattaaa gaacaagtag
     3481 tagaacaagt taaagaagaa aaaacaacta agaaagctac taagaaaaaa gattaattct
     3541 ttttcttata tttattttaa aaagtccatt gcacatttgt aatgggcttt tcctttttta
     3601 agcacttacc tttcccctac tgatttcgcc actcccgaca gcaatctttc cattcccaac
     3661 ggcaatctct tcactcccga cggcaacctt ttcaatcccg acggcaattt ctccgttccc
     3721 gacggcaatc tctccactcc cgatggcaat ctttctattc ccaacggcaa tctctccact
     3781 cccgacggca atttctccgt tcccaacggc aatctcttca ttcccgacgg caatctcttc
     3841 attcccgacg gcaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttccca
     3961 acggcaatct cttcattccc gacggcaatc tcttcattcc cgacggcaat ctcttcattc
     4021 ccgatggcaa tctctcaact ctcgacagca atctcttcat tcccgacggc aatctctcaa
     4081 ctctcgacgg caatttcttc attcccgatg gcaatttctc cgttcccgac ggcaatcgtt
     4141 tcaatccaaa taacaaacat ttttaattat taactttttc tctaaattgt ggaaaaccgt
     4201 aaaatttagg aaaaccactt atttagattt gttaataact agattacaca cttcgttata
     4261 aattgtagga aattattaaa tttgaaatta ttacatacta tagtaattca tggcattaag
     4321 ttggaatgaa ataaaagacc gagcgcttcg tttttcgaaa gaatgggcag atactacccg
     4381 agaggaagcg gatgcaaaac cttttttgga tgcttttttt aatgtgtttg gcattacccg
     4441 aaaaaagatt ggaacctttg aacataaggt aaaaaaaata tcggatgccg atgggtatat
     4501 cgatttgctt tggaaaggta ctattttgat tgaaatgaaa agccgaggtc aaaaccttga
     4561 taaagcattt caacaagcca aagattatac tcatgggctg aaacaaaatg agttgcccaa
     4621 atatatattg gtgtgcgatt ttgatgaatt caggttgtat gataccgaag tgcaaaaaac
     4681 aataactttc aagttgcacg aactgatgga taatgtgcaa cactttggat acattatagg
     4741 gtatcaaaag aaagtttata aagaacaaga cccagccaat attaaagcag ccgaactgat
     4801 gggaaaactg cacgacagat tggaagagat tgggtatgtt gggcatccgt tagaggtgta
     4861 tttggtgcgc attttgtttt gtttgtttgc cgaggatacc actatttttc ataaagaaca
     4921 atttgccgat tatatagtac aacgcactaa tgaagacggc agcgacttgg cagccaaaat
     4981 tcaagagttg tttcaagtac tgaatacgcc tgttgacaaa cgctttaaaa acctagacga
     5041 acaactcaat gagtttccgt atgtaaacgg aaaattgttt gaagaagtat tgcctaccgc
     5101 tagttttgat agcaagatgc gccaagcctt gttagattgt tgctatattg attggagtaa
     5161 aatatcaccg gctatttttg gttcaatgtt tcaaagtgtg atgaacccca aagaacgccg
     5221 caatcttgga gcacattata ccagcgagac taacatttta aaactcatta aacctttgtt
     5281 tcttgacgaa ctttgggcag agtttgaaag catcaaaacc aataaaaaca aacttgccga
     5341 gtttcataaa cgcattagta cgctgaagtt tcttgaccca gcctgtggtt gtggtaactt
     5401 cttggtgatt acctacagag agttgcgctt gctagagttg gaaatactgc gtgctaccta
     5461 taaaagcgga caaggcgtat tggatgtgag ccaaattatt tggttggatg tcgacatgat
     5521 ggcgggtata gaatacgaag aatttccggc acgtatagcc gaagtagcca tgtggttgat
     5581 agaccaccag atgaacttga tgattagcaa tgagtttggg caatattttg cacgcttgcc
     5641 attaaagaaa gcggcaaaaa ttgtacatgg gaatgcgttg cgtgtagatt gggaagaagt
     5701 tgtttctaaa aaagagttgt cttatattat tggtaatccg ccttttatag gtgcagcaat
     5761 gaaaaataat gaacagtctg ccgaaatgga ttttgttttt aataaattat caaataacgg
     5821 caaattggat tatgttgctg catggtattt attagctgca aaatttataa ataaaacaag
     5881 aataaaagct gcttttgtgt ctacaaattc agtaactcaa ggggaacaag ttggtatatt
     5941 atggaatgaa ttaattaaag aatataatat gtgtattcat tttgcacaca gaactttcaa
     6001 ttggaaaaat gatgcgagag gaaatgcagc cgttcattgt gtaattgttg gatttgcaaa
     6061 ttttgaaata actaataaat atttatttga ctatgaggat ataagtggtg aagctactag
     6121 ttataaagtt aaaaacatta gtccttattt atttgatgca tctaacataa taatttctaa
     6181 aactagaatt cctgtatgta aaacaccatt aatgattaga ggaaatattc cttatgatga
     6241 tggaaatctt ttattaaatg aagatgaaaa aaatgaatta atcaatagtg atagtaatgt
     6301 atccagatat attaaaaaat atggtggtgc atatgaaatg ttaaataatg cttggagata
     6361 ttgtttgtgg tttaatgata gttttccacc tgatttaaat aaaaattcaa taattatttc
     6421 aagactaaaa aagtgtaaag aatttagaga aaaaagtaga agcatttctg tctcaagtaa
     6481 atcttcaact ccttatttgt ttggagatat aagacaacca aaatcaaatt atattttatt
     6541 tccacgagtt tcttcaagta aaagaaaata tttgccaata gattattttg aaagcgatat
     6601 tatactagct gatagtggtt atggtctgcc aaatgcaaat ttatatatgt tcggtgtatt
     6661 acattcaata atgcataatt cttggctaag aaatatagga ggtagattaa aaagtgatta
     6721 tagatattca aacaatatcg tctacaacaa ctacccatgg cctgaaaatc caaccgaaaa
     6781 acaaaaacaa gccatagaag acaaagcgca aaaggtgtta gaggtgagag cgagttttcc
     6841 ggagagttca ctggccgatt tgtatcatcc gctaaccatg ccaccggcac tagtaaaagc
     6901 ccacaacgaa ctagacaaag ccgttgatgc cgcctatagc aaacaagact ttagcagcga
     6961 agcaaaacgc atggagtttc tctttgagtt gtatgagaag tacacggcgg ggttgtttgt
     7021 gaaggagaag aagaagaaat gaatgtaatg actacaatag ataccaaata tgaatgcgtg
     7081 ttagactatt tgaaaaggtt aaaatttcaa tatgaatcta actatgataa actcatgttt
     7141 ttttctatta aacctaatga tatcataaaa tatatcacca atcctgaaca atgtggaaat
     7201 tggccatttg aatatgtatg taaaatagaa cacagtataa cacagatttt caaaaagttt
     7261 atagttgact ttccaaaaat tgaattacaa acagaggaag accgcgtaat aagcaaacta
     7321 ttaaaaaaaa atccatgctt tgcttttggc accaatactt tttttgatgg tacaattaca
     7381 gttaatcaga ttattgccta tatggaacga gaagaaaaaa tatatcaatg g
//
LOCUS       DAOOBH010000107         7400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_12003_length_7400_cov_7.154391, whole genome shotgun sequence.
ACCESSION   DAOOBH010000107 DAOOBH010000000
VERSION     DAOOBH010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12003_length_7400_cov_7.154391"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PK218_09660"
     CDS             complement(<1..201)
                     /locus_tag="PK218_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98815.1"
                     /translation="MNDTSKNNSDNQEIDLSQVSKKIGQFFENISTSIFRGFLFLKRN
                     LIIISILFIIGVALGFYLDKTNK"
     gene            complement(210..1898)
                     /locus_tag="PK218_09665"
     CDS             complement(210..1898)
                     /locus_tag="PK218_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98816.1"
                     /translation="MKEIKNISRSKAQESSAAIERLYITMRHLFNRGFYKPMGVSGDT
                     LREALLELSPEIYGSIAEEKVELSGLLYVIERLPVGIEECRYINLTSDEGYSKSHFQA
                     IVPPKRKRNCYRIDEEQMNVEITRGRSDIYDILTHLTFIFIESHKIKDRVLEDEEGKL
                     SRDWQKLEQAVLQTKKLTQVEKEKAISHTANVLGRTFIEVLDIYDSFGTKEKPDRFLH
                     VIYWLGKLAIEEIIDNNKRTITFSPILRERLGHHIHGEIWATRIKQVLKENKLIDRPI
                     HIISANMHSVMNSLFATHVLGTKFKGKSEFDIFQELSKQGAHDVRDKVEAFAVQHGMI
                     SLPDESGTNIDVQIFDTAKIDWKKSAFPTAKIEKEFPVLIVMDYAFGEQAFETIDELL
                     KPFKNGKDKKLINVASVSIMGKAGILQGGKGDIMIPSAHINEGTADNYPFENQLTAEM
                     FEGNGIPVFAGPMITVLGTSLQNKELLKFFHESTWEVIGLEMEGAYYQKAIQSASKIR
                     KSIPENVKVRYAYYASDNPLETGSTLASGGLGTTGVKPTYLITIKILEQIFNIK"
     gene            complement(1941..2801)
                     /locus_tag="PK218_09670"
     CDS             complement(1941..2801)
                     /locus_tag="PK218_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPW98817.1"
                     /translation="MKDKRLLLLLFLITSSVFSQLKGIVVDENNTPIPFVNIWVEGEN
                     IGTSSEESGEFIIHTNNENSRLIFNVLGYEKKIILGKDCKKVILKESSIELKEVFISK
                     RLETKQKTIGKSENKIFEAFPNGPKVDCKFFPFLPEYKKTPYIKYVTLQTDSKIEDAS
                     IRLHFYSVGSDGLPDENLLKKENIVYIKNGVFKHKFDVSNYHLVMPKNGLFVVFEKLM
                     IEKNKLEVTSIDKNSNISKTERLYFPYVLYDYVQRRDKFIFFGGKWSKEENEEKMKIY
                     EPAINLVLTN"
     gene            complement(2798..4306)
                     /locus_tag="PK218_09675"
     CDS             complement(2798..4306)
                     /locus_tag="PK218_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPW98818.1"
                     /translation="MSIKAIAAKLFAKKIHLQTQKWVSKPVETQQKVFEDLIKQARET
                     AFGKDHNFSKIKTFKDFSAQVPVRDYEALKPYVDRVVKGEENILWKGKPLYFAKTSGT
                     TSGAKYIPLTAASMPYHIEAARNAILSYIHETGKADFVDGKMIFLQGSPILEEKNGIK
                     LGRLSGIVAHFVPKYLMKNRMPSWETNCIEDWETKVNAIVEETITENMSVISGIPSWV
                     QMYFEKLEQKTNKPVGDIFRNFNLFIYGGVNYEPYRAKFENLIGRKVDSIELFPASEG
                     FFAYQDSQHEKGMLLLLNSGIFYEFIKADEFYTENPKRYTIGEVVLNMNYVLIISTNA
                     GLWGYNIGDTVQFTSLKPYRVIVSGRIKHYISAFGEHVIGKEVESALQLAMQNSNISI
                     NEFTVAPQITPKEGLPYHEWFIEFENEPEKLEDFALQIDDEMRKQNAYYDDLIVGKVL
                     RKLVITKVSKNGFQDYMKSIGKLGGQNKLPRLANDRKIADYFENRAQNKENR"
     gene            complement(4309..4674)
                     /locus_tag="PK218_09680"
     CDS             complement(4309..4674)
                     /locus_tag="PK218_09680"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HPW98819.1"
                     /translation="MDIINIVSTNMMGMPFFIGFIFCVTGLIMYFFPPKKINHFYGYR
                     TSASMKSQEVWDFSQRISSIKMIQSGLLLILISGLKLFIPFDIGQELITSLVSIAIVI
                     LLLFFTVERDIKKKFTNQE"
     gene            complement(4677..5549)
                     /locus_tag="PK218_09685"
     CDS             complement(4677..5549)
                     /locus_tag="PK218_09685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW98820.1"
                     /translation="MSEKRLKRKKIKKKLFTKNRLVILNEDTFEELFSLRLTMMNVFV
                     VATLGAIIIIFVTTFIIAFTPLREYIPGYASSKLKKDATELALKSDSLTKRIQENDAY
                     IKSIKKILTGELEYAKFNKDSILAVATTEVVDEKEMLPDEEELKLREEVAQEDKYNLF
                     EKAKPKVTTVFFPPAKGLITEKFNAREKHYAIDVALAKNTPIKAILTGKVIFADWTPT
                     TGNVIIIRHNNGFISVYKHAASLTKSQGDAVRTGEVIALAGSTGQESTGIHLHFELWK
                     DGYPIDPTVFINFE"
     gene            5719..5901
                     /gene="tatA"
                     /locus_tag="PK218_09690"
     CDS             5719..5901
                     /gene="tatA"
                     /locus_tag="PK218_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPW98821.1"
                     /translation="MGRLGMPEILVILVVVLLLFGGKKIPELMKGLGTGIKEFKDAAK
                     GEDEAKATSKKEETKE"
     gene            6007..7386
                     /locus_tag="PK218_09695"
     CDS             6007..7386
                     /locus_tag="PK218_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPW98822.1"
                     /translation="MKKIILLLILISGYASVFSQEIIGSWAGELNVQGRKIPLIFNIK
                     KIGDTYESTADSPMQGAKDIPIDNTIFSNDELLLDAKKLGFQYKGKFENQTITGTFSQ
                     GGMTIDLVLSRKKENEFELKRPQEPKTPFNYIIQEVTFTNPKDNNILAGTLTLPKDKK
                     EFPIAVLITGSGQENRDSEIFGHKPFWVIADDFAKKGIGVLRIDDRGIGGSSKGNNND
                     TSENFAGDINTAVDFLSEKGYKNIGLIGHSEGGLIAPMVAAKNKNVKFLISMAGPGVS
                     IDELLLLQTKALSKSNGASEEEIIATSAFNKKLYSFIKNYDGTNLETEIRPIFIEELK
                     KSFANELPSETELNQYVDDQMQQITNPWFVYFIKSNPQDYWSKLKIPVLAINGTKDLQ
                     VIATENLAGIKKALEKAGNKKFEIKSFEGLNHLFQTATTGAVSEYVTIEETISPAVLN
                     TMSNWILKQ"
BASE COUNT         2465 a         1316 c         1029 g         2590 t
ORIGIN      
        1 tttattagtt ttatctaaat agaaaccaag agccactcca attataaata atattgaaat
       61 tataattaaa tttcttttca aaaaaagaaa tcctctaaaa atagaagtag aaatgttttc
      121 aaaaaattgt cctatttttt ttgatacttg cgataaatca atctcttgat tatccgaatt
      181 gtttttagac gtatcgttca tataaaaatt tattttatgt taaaaatttg ttctaatatt
      241 ttaatagtaa tcaaatatgt gggtttaact cccgtagttc ctaagccacc agatgctaat
      301 gtacttcctg tttccaatgg attatcagat gcataataag catatcttac ttttacattt
      361 tctggaatac tttttctaat ttttgaagct gattgaatgg ctttttgata ataagcacct
      421 tccatttcca gtccgattac ttcccaggta gattcgtgga agaattttaa taactctttg
      481 ttttgaagcg atgttccaag aacggttatc attggtccgg caaaaacagg aatgccattt
      541 ccttcaaaca tttcggctgt taattgattt tcgaatggat aattgtctgc tgttccttca
      601 tttatatgtg ctgatggaat cataatatcg ccttttccac cttgaagaat tcctgctttt
      661 cccatgatgg aaaccgacgc tacattgatt aattttttgt cttttccatt tttaaagggt
      721 tttaacaatt catctatggt ttcaaaagct tgttctccaa aagcataatc cataacgatt
      781 aataccggaa attctttctc aatttttgct gttggaaatg ccgatttttt ccaatcaatt
      841 tttgcagtat caaaaatttg tacatcaatg tttgttcccg attcgtctgg aagtgaaatc
      901 attccgtgct gaacagcaaa tgcttctact ttatctcgaa catcatgtgc gccttgtttg
      961 cttaattctt gaaaaatatc aaactctgat tttcctttaa actttgttcc taaaacatgg
     1021 gtagcaaata acgaattcat cacactatgc atattggcac ttatgatatg aattggtcta
     1081 tcaatcaatt tgttttcttt taaaacttgt ttgattcttg ttgcccaaat ttctccatga
     1141 atatgatgtc ctaatcgttc tcttaaaata ggactaaacg taatagttcg tttattattg
     1201 tctattattt cttcgatggc caattttcct aaccaataaa ttacatgtaa aaagcggtct
     1261 ggtttttctt tggttccaaa tgaatcgtag atgtctaaca cttcaataaa agttcttcct
     1321 aatacatttg ctgtatgtga aatggctttc tctttttcaa cttgtgttaa ttttttagtt
     1381 tgaagaacag cttgttctaa tttttgccaa tctcgcgata attttccttc ttcatcttct
     1441 aaaactctgt cttttatttt atgtgattct atgaaaataa aagtcaaatg cgttaaaata
     1501 tcgtagatgt ctgatcgacc acgagtgatt tcaacattca tttgttcttc atctattcgg
     1561 taacaatttc gttttctctt tggtggcact attgcctgaa aatgcgactt agaataaccc
     1621 tcatctgatg ttaaattaat ataacgacat tcttcaattc caacaggtaa tctttctata
     1681 acataaagca aaccacttag ttctactttt tcttctgcaa tacttccata aatttctgga
     1741 ctaagctcta acaaagcttc tcttaaagta tctcccgaaa cacccattgg tttatagaaa
     1801 cctctgttga ataaatgtct catggtaatg tacaatcgtt cgatagctgc cgacgattct
     1861 tgcgctttgg agcgcgaaat gtttttaatt tctttcatta aattgttttt actaacccgc
     1921 aaagatagtt tttttatagt ttagttggtt aaaacaaggt taattgctgg ttcgtatatt
     1981 ttcatttttt cttcgttttc ttctttactc cactttcctc caaaaaagat aaacttgtct
     2041 cttcgctgta cataatcata aagtacgtag ggaaaataaa gtcgttctgt ttttgaaatg
     2101 ttactattct tatctattga agtgacttcc aacttgtttt tttcaatcat cagtttctca
     2161 aaaactacaa acagtccgtt cttaggcatc actaaatgat agttggatac gtcaaactta
     2221 tgtttaaaaa cgccattttt aatataaaca atattctcct tttttaataa attctcgtct
     2281 ggcaaaccat cacttccaac gctataaaaa tgcaatctta ttgaagcatc ttctatttta
     2341 ctatctgttt gtagcgttac atatttaata taaggtgtct ttttatattc tggtaaaaaa
     2401 ggaaaaaatt tacaatctac tttgggtcca tttggaaatg cttcaaatat tttgttctca
     2461 cttttaccaa tcgttttttg tttggtttcc aatcgttttg aaataaatac ttctttcagt
     2521 tcgatggatg attcttttaa aattactttt ttacagtctt tgcctaaaat aattttcttt
     2581 tcatatccta aaacattaaa aatcaaacga ctattttcat tattggtatg aataataaac
     2641 tcgccacttt cttcgctcga agtgccaatg ttttcgcctt caacccaaat atttacaaat
     2701 ggaattggag tgttattttc gtcaacaaca atgcccttta gctgactaaa aaccgaactt
     2761 gtaattaaaa acaacagcaa tagtagtctt ttatctttca tctattttct ttgttctgtg
     2821 ctctattttc aaaataatca gcaatctttc tatcatttgc caatcgtggt aatttatttt
     2881 gtcctcctag ttttccaatc gatttcatat agtcttgaaa accattttta gaaactttag
     2941 tgatgactaa ttttcgcaac actttgccca caattaaatc atcgtaatag gcattttgtt
     3001 tgcgcatttc gtcatcaatt tgcaaggcaa aatcttctaa tttttcaggt tcattttcaa
     3061 actcaataaa ccattcatga taaggtaatc cttcttttgg cgtaatttgt ggcgcaacgg
     3121 taaattcatt aattgatatg tttgaatttt gcattgctaa ttgtaacgcg ctttctactt
     3181 ccttaccaat aacatgttcg ccaaaagctg aaatataatg cttgattctt ccagaaacga
     3241 taactcgata aggtttcagt gaagtaaact gaacggtatc tccaatatta tatccccaaa
     3301 gtccggcgtt ggtagaaatg attaaaacat aattcatgtt aagtactact tcgccaatag
     3361 tatatcgttt tggattttca gtataaaatt catcagcctt tataaattca taaaaaatac
     3421 ccgaattcaa caacaacaac attccttttt cgtgttgtga atcctgataa gcaaaaaatc
     3481 cttccgaagc cggaaacaac tcaatagaat ctacttttcg tcctattaga ttttcaaatt
     3541 tggctctata aggttcatag tttacaccgc cgtagataaa caaattaaag ttcctaaaaa
     3601 tatccccaac aggtttattg gtcttttgtt ctagcttttc aaagtacatc tgaacccacg
     3661 acggaattcc cgaaatcacc gacatgtttt cggtaatagt ttcttcaact atagcattta
     3721 ctttggtttc ccaatcttca atacaattgg tttcccaact aggcattcta tttttcatca
     3781 aatattttgg aacaaaatgt gctacaattc cagaaagtcg tccgagctta atcccgtttt
     3841 tttcttccaa aatcggacta ccttgcaaga atatcatttt gccatctaca aaatctgctt
     3901 ttcctgtttc gtgaatgtaa ctcaaaatag catttcgagc tgcttcaata tgataaggca
     3961 tcgatgctgc cgttaatgga atatattttg cacccgaagt cgtacccgaa gtttttgcaa
     4021 aatacaatgg ttttcctttc caaagtatgt tttcttcacc tttaacaaca cgatctacat
     4081 aaggctttaa tgcttcataa tctcgaactg gaacttgagc cgaaaaatct ttaaaagtct
     4141 ttatttttga aaaattgtgg tctttcccaa atgctgtttc acgcgcttgc tttatcaaat
     4201 cttcaaaaac tttttgttgt gtttccacag gttttgaaac ccatttttgg gtttgaagat
     4261 gaatcttttt agcaaataat ttagcagcaa ttgcttttat tgacatactt attcttgatt
     4321 agtaaatttt ttcttaatgt ctctttcaac cgtaaaaaac aataataata taacaatcgc
     4381 aattgaaacc aaacttgtta ttaattcttg tccaatatca aaaggaataa acaattttaa
     4441 tccagaaatc agaatcaata acaaccctga ttgtatcatt tttatagaag aaattcgttg
     4501 tgaaaaatcc caaacttctt gcgatttcat agatgcactc gttcgataac cataaaaatg
     4561 attaatcttc tttggtggaa aaaaatacat aatcaaaccc gtaacacaaa aaataaagcc
     4621 tataaaaaaa ggcattccca tcatattggt tgacactatg ttaatgatat ccataattat
     4681 tcaaaattaa taaaaaccgt tggatcaatc ggataaccgt ctttccaaag ctcaaaatgc
     4741 aaatgaattc ccgtcgattc ttgaccagtc gaaccagcaa gcgcaataac ttctccagtt
     4801 ctaacagcat ctccttgcga tttcgtcaac gaagcagcgt gtttataaac cgaaataaat
     4861 ccattattat gacgaataat aattacattt ccagtagttg gagtccaatc tgcaaaaatt
     4921 acttttcccg tcaaaattgc ttttataggt gtgttttttg ccaaagctac atcaatggca
     4981 tagtgctttt ccctagcatt aaatttttca gtaatcaatc cttttgcagg tggaaaaaat
     5041 actgtagtta ccttcggttt tgccttttca aaaagattgt acttatcttc ttgcgctact
     5101 tcttctctaa gtttcaattc ttcctcgtcg ggcaacattt ctttttcatc aacaacttct
     5161 gtcgtagcta ctgccaaaat agaatcctta ttaaacttag catattccaa ctctccagta
     5221 agtatctttt taattgattt aatataagca tcgttttctt gaattcgttt cgttaaagaa
     5281 tccgatttca aagccaattc cgttgcgtct tttttaagtt ttgaagaagc ataaccagga
     5341 atatattcgc gcaaaggtgt aaatgcaatt ataaaagtag ttacaaaaat gataataata
     5401 gcacccaaag tggccaccac aaaaacattc atcatcgtta atcgcaaaga aaacaattct
     5461 tcaaaagtat cttcattcaa aataaccaat cggtttttgg taaagagttt tttcttgatt
     5521 tttttacgtt ttaaacgttt ctcagacatg atttttgatt gtgttacaaa tatagaaaat
     5581 aatactcgat actatttcct atgaataacg aactttaaca ccatttattt tctataaatc
     5641 aaaaagaata tcaatttctt tttacctttg cctaacttta ttacaaataa aatctgcgaa
     5701 agcataaaat aaaatatcat gggaagatta ggaatgcctg aaatattagt aattctagta
     5761 gttgtattgc ttctttttgg aggtaaaaaa attccagaac taatgaaagg tttaggaaca
     5821 ggaatcaaag aatttaaaga cgctgcaaaa ggtgaagatg aagcaaaagc gacttctaaa
     5881 aaagaagaaa ctaaagagta acctttttta atttacaaaa cagtaaaccc caaaatacaa
     5941 ttcataaaaa aattgatgtt tggggttttt ttatctataa atacccactg gctaatctta
     6001 tcataaatga aaaaaataat tttactattg attttaatct caggatatgc atctgttttt
     6061 tcacaagaaa ttataggctc atgggctgga gaactaaatg ttcaaggtag aaaaatacct
     6121 ttaattttca acattaaaaa aataggcgat acctatgaat ctaccgcaga tagcccaatg
     6181 caaggtgcaa aagatattcc tatagacaac acaattttta gcaacgatga attactttta
     6241 gatgcaaaaa agttaggttt tcaatacaaa ggaaaatttg aaaaccaaac catcaccggt
     6301 actttttccc aaggcggaat gactattgat ttggttcttt ctagaaaaaa agaaaatgaa
     6361 tttgaactaa aaagacctca agaaccaaaa actcctttca actacattat tcaagaagtt
     6421 acttttacaa atccaaaaga caataatatt ctcgcaggaa cattaacact tccaaaagac
     6481 aaaaaagaat ttccaattgc tgttttaata accggctctg gtcaagaaaa tagagattca
     6541 gaaatttttg gtcataaacc attttgggta attgcagatg attttgccaa aaaaggaatc
     6601 ggtgttttac gaattgacga tagaggaatt ggcggttcaa gcaaaggaaa taacaacgat
     6661 acttccgaaa attttgcagg tgatataaac actgctgtcg attttcttag tgaaaaaggt
     6721 tacaaaaaca tcggactaat aggtcatagc gaaggcggat taattgcacc aatggtagcc
     6781 gcaaaaaaca aaaatgtaaa atttttaatt tcaatggctg gtccaggtgt ttctattgat
     6841 gaattattgc ttttacaaac taaggctctt tctaagtcaa atggtgctag tgaggaagaa
     6901 ataatagcta ccagcgcatt caataaaaaa ttatattctt ttattaaaaa ttatgacgga
     6961 actaatttag aaacagaaat tagacctata tttattgaag aattaaaaaa atcatttgca
     7021 aacgaattgc caagtgaaac tgaactcaat caatatgttg acgatcaaat gcaacaaata
     7081 accaatcctt ggtttgtcta ttttataaaa agcaatccac aagattattg gtcaaaacta
     7141 aaaatacccg tattagcaat aaacggcaca aaagatttac aagtaatcgc cactgaaaat
     7201 ttagctggaa ttaaaaaagc attagaaaaa gcaggaaaca aaaaatttga aatcaaatct
     7261 ttcgaaggtt taaatcatct atttcaaacc gcaactactg gagccgtttc agaatacgta
     7321 accatcgaag aaacaatttc tccagcagtt ctaaacacca tgagcaactg gattttaaaa
     7381 caataacaat agttataaaa
//
LOCUS       DAOOBH010000108         7262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_12267_length_7262_cov_4.628278, whole genome shotgun sequence.
ACCESSION   DAOOBH010000108 DAOOBH010000000
VERSION     DAOOBH010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12267_length_7262_cov_4.628278"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK218_09700"
     CDS             <1..258
                     /locus_tag="PK218_09700"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252349.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPW98823.1"
                     /translation="KDIELIELNEAFASQSLAVIRALNLNPEIINVNGGAIALGHPLG
                     CTGAKLSVQLFDEMKRRGNKYGMVTMCVGTGQGAAGIYELL"
     gene            279..2069
                     /locus_tag="PK218_09705"
     CDS             279..2069
                     /locus_tag="PK218_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPW98824.1"
                     /translation="MEDITRGGQFLVKETKCENVFTPEDFSEEQIMMRESVKEFVDKE
                     IWPNKNRFEAKDYAFTEEVMKKAGEMGFLSVSVPEAYGGMGMGFVDTCLVCDYISGAT
                     GSFSTAFGAHTGIGTMPITLYGTEEQKQKYVPKLASGEWFGAYCLTEPGAGSDANSGK
                     TKAVLSDDGKHYNITGGKMWISNAGFCSLFIVFARIEDDKNITGFIIENDPSNGITLG
                     EEEHKLGIRASSTRQVFFANTKVPVENMLAGRGEGFKIAMNALNVGRIKLAAACLDAQ
                     RRVTSNAVNYANERIQFNVPISSFGAIRYKLAEMATSAYAGESATYRAAKDIENRIKL
                     REAEGTSHQEAELKGVEEFAIECSILKVAVSEDVQNCADEGIQIYGGMGFSEDTPMES
                     AWRDARIARIYEGTNEINRMLSVGMLIKKAMKGHVDLLGPASKVQEELMGIPSFDTPD
                     YSELFTEEKELISKLKKAFLMVAGGAVQKYGMDLDEHQQLLMASADILIEIYMAESTI
                     LRTEKLAKKEGEAKVQEQIAMAKLYLYKAVDIIAQKGKESIISFAEGDEQRMMLMGLR
                     RFTKYTNMPNIVALRETITAKLVAENSYCF"
     gene            2194..3843
                     /locus_tag="PK218_09710"
     CDS             2194..3843
                     /locus_tag="PK218_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98825.1"
                     /translation="MLLLLVSQFALFFLLIGWGNLFEKLLPNLWNGLSTKLVTGIISI
                     SLCSSIIGFFLPLNVYYEIGIILIGIIGLVKNHRTISSDLKYLKDKTFLFFAFIVIVI
                     STFAPFINDHFGYYVSTIKWLNEFGLVKGISNISLVLGQQSTWHVFQASIDTLVDPFM
                     RVNATLLLIFILYSIENNRKELLILTPLFLLFIHSPSPDLIIYIVSLIITLELLKANP
                     NYASLWLVSLFLMTIKPIIFWLPLLIFILSLKTNIKTLFKFKNIVFSLFIVLPFFAKQ
                     LWCFGDILFPLQTNLFDLDWKPNTLLLELSNKNALLKTYDFQYTIKEITSCNALDKIF
                     KWFTLSGFKPIIHILIVISILVFGLYSFLKKKKTYILLWLCIVIKTIVIFQFSGQYRF
                     MLDGVLVLSILLIISLQIPSKYYLWISYFGCFIVFSIFLFPSLIKQNISSFYVGQLMG
                     NPELKQVYEPLNYSIADYSKNKITNFEFYTPNNYHLMLDVPIPCLIPIALDEFRYVND
                     FPVAYDKDNLQKGFYHKTLTLKQKEKLINLLKNYTVYYPLD"
     gene            3899..4423
                     /locus_tag="PK218_09715"
     CDS             3899..4423
                     /locus_tag="PK218_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HPW98826.1"
                     /translation="MKKIVLALIIASGIIVACKSTTNTSDKELTITLEPKSGSSVNGT
                     ATFTEKNGEVTLLAKLTGLTPGVHAIHIHEKADCSAADGTSTGGHWNPTFKKHGKFGI
                     GEYHRGDIGNFTADVDGNATVFFKTNEWAIGGTDETKNILGKGLIVHKDPDDFTSQPT
                     GNAGARVACSGIIK"
     gene            5026..5634
                     /locus_tag="PK218_09720"
     CDS             5026..5634
                     /locus_tag="PK218_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98827.1"
                     /translation="MKKIILFIILILNLTSNAQCPTPSNLIASGGLGQTTLSWTENGI
                     ATEWEIAVIPNYSIGDPIPNFGIITTSNPFILTGLAPVCNAFFVRSVCNSTNFSPWAV
                     IGEGCSLNTYIWLTTLSNDIFETNSEKNRLQIYPNPTKNIIHIINNSELEKIKIFDCL
                     GKVVLTQTQNNTEINVENLSKGIYLIEIQTENEKVYKKFIKE"
     gene            5884..>7262
                     /locus_tag="PK218_09725"
     CDS             5884..>7262
                     /locus_tag="PK218_09725"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPW98828.1"
                     /translation="MKKITLLLLLLSSYISLGQDFYDLNTIQEIRITFSQTNWDAVLD
                     AAEASDAYTSATSISINGAMYNNVGVKYKGNSSYNANQVKNPLHIELDTYIDQNYLGY
                     TDIKLSNVFVDPTFVRETLGYKILGNYMDVPKANYAKVYINEAYIGLYTNVESISKKF
                     VDSRFGSKTNAFFDCSPPAGAGPQTTNLPNLAYLGTSSSSYTSAYDMKSDTGWDDLIA
                     LTSTLSSTNSTNTATIEAVLDVDRALWMLAFDNVFVNLDSYIGQFKQNYYLYKSDNGQ
                     FMPIVWDLNMCFGVFGMTGSGGNLTTATKKTLSHTLHSNESAWPLVQKLLAVPSYKKK
                     YIAHYKTIVSEVVSASGSNYFLTDAQAMQTLISNAVATDVNKFSYQTTANFTNNLNST
                     DVNVSNNTAPGITGLMSARNTYLSALPDFTNTQPTISNIVLSNVAPLIGTSVNVTANV
                     SNTNYVYLG"
BASE COUNT         2542 a         1100 c         1215 g         2405 t
ORIGIN      
        1 aaagacattg aactaattga gttgaacgaa gcctttgcat cacaatcatt agctgtaatt
       61 agagctttaa acctaaatcc cgaaatcatt aacgtaaatg gtggtgcaat cgcattaggt
      121 catccattag gttgcacagg agccaaacta tcagtgcaat tatttgacga aatgaaacgc
      181 agaggaaaca aatatggaat ggtaaccatg tgcgtaggaa caggtcaagg tgccgctggt
      241 atttatgaat tactataatt aataataaaa ctataaaaat ggaagatata acaagaggtg
      301 gtcagttttt ggtaaaagaa accaagtgtg aaaatgtctt tacacctgaa gactttagcg
      361 aagagcaaat catgatgcgt gaaagcgtta aggaatttgt ggataaggaa atttggccta
      421 ataaaaatcg ttttgaggca aaagactatg cttttactga agaagtaatg aaaaaagcgg
      481 gcgaaatggg ctttttaagc gtttctgttc ctgaagctta tggcggaatg ggaatgggat
      541 ttgtggatac ttgtttagtt tgtgattata tttctggtgc aacaggctct ttttcaactg
      601 cttttggtgc gcatactgga attggaacaa tgccaattac attgtatgga accgaagaac
      661 aaaaacaaaa atacgttcca aaattagctt ctggcgaatg gtttggagct tattgtttaa
      721 cagaacctgg cgcaggaagt gatgccaatt ctggaaaaac taaagctgtt ctttcagatg
      781 atggcaaaca ttacaatatt actggaggaa aaatgtggat ttctaatgca ggtttttgtt
      841 ctttatttat agtttttgct cgtattgaag atgataaaaa tattactggt ttcattatag
      901 aaaacgatcc atcaaacgga attacacttg gtgaagaaga acacaaattg ggaattagag
      961 cttcctctac ccgacaagtt ttctttgcta acacaaaagt accagttgaa aatatgttgg
     1021 ctggacgtgg cgaaggtttt aaaatagcta tgaatgcctt aaatgttggt cgtatcaaat
     1081 tggcagccgc atgtttggac gctcaacgtc gtgttacttc aaatgcggtt aattatgcca
     1141 atgaaagaat tcaatttaat gtgcctattt caagttttgg tgctattcgt tataaattgg
     1201 ccgagatggc tacttctgct tatgctggag agagtgctac ctatcgtgct gcaaaagaca
     1261 ttgaaaatag aattaagctt cgtgaagcag aaggcacatc gcatcaagaa gcggaattaa
     1321 aaggtgtaga agaatttgcc atcgaatgtt ctatcttaaa agttgctgtt tctgaagatg
     1381 tgcaaaactg tgcagacgaa ggaattcaaa tctatggtgg aatgggattc tcagaagaca
     1441 cacctatgga aagtgcttgg agagatgcgc gtattgctag aatttatgaa ggtacaaatg
     1501 aaattaacag aatgctatcc gttggaatgt taatcaaaaa agctatgaaa ggtcacgttg
     1561 acttacttgg tcctgcttca aaagtacaag aagaattaat gggaattcca tcgtttgaca
     1621 cgcccgatta ttctgaatta ttcacagaag aaaaagaatt aattagcaaa ttgaaaaaag
     1681 cattcctaat ggttgctggt ggtgccgttc aaaaatatgg aatggattta gatgaacacc
     1741 aacaattatt aatggcttca gctgatatat tgattgaaat ttacatggct gaaagtacta
     1801 ttctaagaac agaaaaatta gccaaaaaag aaggcgaagc taaagttcag gaacaaattg
     1861 ccatggcaaa actatactta tacaaagccg ttgatataat tgctcaaaaa ggaaaagaaa
     1921 gcatcatttc ttttgccgaa ggcgatgaac aacgcatgat gttaatgggt ttacgccgat
     1981 ttacaaaata tactaatatg ccaaacattg ttgctttaag agagacaatc acagcaaaat
     2041 tagttgcaga aaatagttat tgcttttaag cattgtctat aattttaagt taagttaatt
     2101 gtttgaaacc acttgaatgt catattcagg tggttttttt attatttata gataatttat
     2161 tttagatttg aaatcaataa gtaatttata aacatgcttt tacttttagt ttctcaattt
     2221 gcattgtttt tccttttaat tggatgggga aatttatttg aaaaactcct acccaacttg
     2281 tggaatggac tatcaacaaa gcttgtaaca ggcattatat ctataagttt atgctcttca
     2341 attattggtt tttttctacc gttgaatgta tattatgaga taggaataat tctgataggt
     2401 ataattggat tagtcaaaaa tcatagaaca atttcatccg atttaaaata tctgaaagac
     2461 aaaacatttc tgttttttgc tttcattgtt attgtaatta gcacttttgc tccttttata
     2521 aacgaccatt ttggttatta tgtttccact attaaatggc tgaatgagtt tggattagtg
     2581 aaaggaattt caaatattag tttagtatta ggacaacaat ctacatggca tgtttttcaa
     2641 gcgagtattg atactcttgt tgatccattt atgagagtaa atgcaacact gctacttatt
     2701 tttattctgt acagcattga aaacaatcga aaagaattac tcattttaac acctttattt
     2761 cttttattca ttcactcgcc ttcacccgat ttaataattt acatagtatc actaataatc
     2821 accttagaac ttttaaaagc caatccaaat tatgcttctt tatggctagt tagtttattt
     2881 ttaatgacca taaaaccaat cattttttgg ttaccactac tgatttttat actttcatta
     2941 aaaacaaaca tcaagacttt atttaagttt aaaaacatag tcttttctct ttttattgtt
     3001 cttccttttt ttgcaaaaca gttatggtgt tttggcgata ttttatttcc actgcaaaca
     3061 aatttattcg atttagattg gaaaccaaac acattgcttc ttgaattatc caataaaaat
     3121 gcacttttaa aaacatacga ttttcaatat accatcaaag aaataacatc atgtaatgct
     3181 ttagataaaa tatttaaatg gtttacttta agtggattta aacccataat acatatttta
     3241 attgttattt caattttagt ttttggtttg tattcttttc ttaaaaaaaa gaaaacctat
     3301 attcttttat ggttgtgcat cgttataaaa acaattgtaa tttttcaatt ttcaggacaa
     3361 tatcgtttta tgttagatgg cgttttagtg ctgtctattt tactaatcat ttcgcttcaa
     3421 attccttcta agtattattt gtggatttct tattttggat gctttatagt gttttcaatt
     3481 ttcttattcc cttccttaat aaaacaaaac attagctctt tttatgtggg gcaacttatg
     3541 ggtaaccctg aattaaaaca ggtttatgaa ccactgaatt atagtatagc tgattattct
     3601 aaaaacaaaa tcaccaactt tgagttttat actccaaaca actatcattt aatgttagac
     3661 gtacccattc catgtcttat tccaattgct ttagatgaat ttagatatgt taatgatttt
     3721 ccagttgctt atgataaaga caatcttcaa aaagggtttt atcacaaaac attaacgcta
     3781 aaacaaaaag aaaaattgat taatctttta aaaaactata ccgtttatta tcctttagat
     3841 taacatttct ttttaattta taaggagata tttttgtaaa tttgaaaaaa tctatatcat
     3901 gaaaaaaata gttttagcat taattattgc atctggaata atagtagctt gtaaatcaac
     3961 tacaaataca agcgataaag aattaaccat taccttggag cctaaaagtg gaagttctgt
     4021 aaatggaacg gcaactttta ccgaaaaaaa tggagaagta actttgttgg ccaagctaac
     4081 aggactaaca cctggcgtac acgccattca catacatgaa aaagcagatt gctctgccgc
     4141 agatggtact tctactggtg gacactggaa cccaacgttt aaaaaacacg gaaaatttgg
     4201 cattggcgaa taccatcgtg gtgatattgg taattttaca gccgatgtag atggaaatgc
     4261 aaccgtattt tttaaaacca acgaatgggc aattggcgga accgatgaaa ctaaaaacat
     4321 tcttggcaaa ggtttaatcg ttcacaaaga cccagatgac tttacttcgc aaccaacagg
     4381 gaatgctggt gcaagagtag cttgttcggg aattatcaaa taaacgttga agaaaaacgt
     4441 tttttaaact tcatagttta aacaccacaa aaaacacaac aacaaaagta aaaaaaaggt
     4501 ctaaaaacgc attttgtttt taggcctttc tgcttgtcta tttctgcatt tattaatagc
     4561 ctacaatgat gctaacctga ttactccaca ctcctattat agtatcttta tacaaacaca
     4621 tcgctttgta ttcccaaaaa acacttccta tttgccaaaa attgaagaaa cttttaaaaa
     4681 aaatacgctt aaagtttggg atataagatt tccaaaatta attgatgaaa tacctataag
     4741 tatcgtattt actaatttaa gtaacgaatt atgcatataa attgaagaaa acaacataaa
     4801 aggttgggca aagggaaaac tagaaaactc agaatttatt gcgtgaaatt ccagtgatgt
     4861 gttgcatggt taaaaataca gaaaactgtt ttttcgtaca actttttagt atatttgttt
     4921 actgtgaaga gaaagttttt ttcacagtct gacgttggca gaaaccttag aaaaactgaa
     4981 cgataaaaaa gtaatgaata ttaataatct aaataagtaa aaattatgaa gaaaataata
     5041 ctgtttatca ttttaatatt gaatttaaca agtaatgcac aatgtccaac tccatcaaat
     5101 ttaattgctt ccggaggttt aggtcaaacc actttgagtt ggactgaaaa tggaatagct
     5161 accgaatggg aaattgctgt aataccaaat tatagtattg gagatccgat accaaatttt
     5221 ggaataatta ccacaagtaa tccatttatc ttgactggtc tagcaccagt ttgtaatgcg
     5281 ttttttgtta ggtctgtatg taattcaacc aattttagtc cttgggctgt tattggtgaa
     5341 ggttgttctc ttaatactta tatttggctt acgactttat ctaatgatat tttcgaaact
     5401 aattcagaaa aaaacagatt acaaatttat cctaatccaa caaaaaatat tattcacata
     5461 ataaacaact ccgaattaga aaaaataaaa atatttgatt gtttgggaaa agtagttttg
     5521 acgcaaactc aaaacaatac tgaaattaat gtagaaaatc tttctaaagg aatttactta
     5581 atagaaattc agacagaaaa cgaaaaagtt tataaaaaat tcataaagga ataaataaaa
     5641 attaaaaagg catctgccaa cagctaaggc ttcgtcgcgt tcgccacggc gaacgaacaa
     5701 tgaatccggt gttgaacgca acctctacca aaaaaaatta taaaaacaaa cctcgtttag
     5761 tgcattctaa gcgagttttt tttttaataa ttacactgat ttcaaaaaaa taattggaac
     5821 tgtgcaagaa ataaatttta tattcgtcta atcatataca attaatagat acataattta
     5881 aacatgaaaa aaattacatt actcctactg ttgcttagtt cttacatttc attgggacag
     5941 gatttttatg atttaaacac cattcaagaa atcagaataa ctttttcaca aaccaattgg
     6001 gatgctgttt tagatgctgc agaagcttcg gatgcttaca cttctgcaac atccatttcc
     6061 ataaacggag cgatgtataa taatgttggg gtgaaataca aaggaaatag ttcttataac
     6121 gctaatcaag ttaaaaatcc tttgcatata gaattggata cttatattga ccaaaattat
     6181 ttgggatata ccgacataaa attaagtaat gtctttgttg atcctacatt tgttagggaa
     6241 acattgggct ataagatatt aggaaattat atggatgtgc caaaagctaa ctatgcaaaa
     6301 gtatatatta acgaagctta cattggttta tatactaatg tggaatccat ttctaaaaag
     6361 tttgtagata gtagatttgg ttcaaaaacg aatgcttttt ttgattgtag tccgcctgct
     6421 ggagctggac ctcaaacaac taatctgcct aatttagcgt atttaggaac aagtagttca
     6481 agttatactt ccgcttatga tatgaaatca gatacgggtt gggatgactt aattgcttta
     6541 actagcactc tatcttctac aaactctaca aatacagcta ctattgaagc cgttttagat
     6601 gttgacagag ccttgtggat gttagccttt gataatgttt ttgtaaacct agacagctat
     6661 attggtcaat ttaaacaaaa ctattattta tataaatcag acaatggtca gtttatgcca
     6721 attgtatggg atttgaatat gtgttttggt gtttttggta tgacgggttc tggtggaaac
     6781 ttaactactg ccaccaaaaa aacgttatca catactttgc actcaaatga atctgcttgg
     6841 cctttagttc aaaaattatt agcagttccg tcctataaga aaaaatatat tgctcattat
     6901 aaaacaatag tatccgaagt agtttctgct agtggtagta attacttttt aaccgatgct
     6961 caagcgatgc aaactttaat aagtaatgct gtggctaccg acgttaataa atttagctat
     7021 caaacaacgg ctaattttac aaataaccta aattcaactg atgttaatgt atccaataat
     7081 actgctccag gaataactgg acttatgtcg gctagaaata cttatttatc tgcattgccc
     7141 gattttacaa atacgcagcc aactatatca aatattgttt tatctaatgt tgctccacta
     7201 attggaacat cagtaaatgt tactgcaaac gtttcaaata ctaactatgt ttacttagga
     7261 ta
//
LOCUS       DAOOBH010000109         7220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_12372_length_7220_cov_6.592045, whole genome shotgun sequence.
ACCESSION   DAOOBH010000109 DAOOBH010000000
VERSION     DAOOBH010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12372_length_7220_cov_6.592045"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..2991)
                     /locus_tag="PK218_09730"
     CDS             complement(313..2991)
                     /locus_tag="PK218_09730"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPW98829.1"
                     /translation="MSKTTDYISNTMSLREPLKKSLELLDSLIAKTDLIGNSKTEKKI
                     ESIKKDCSLFEEFERGFPSVCFSIATGIGKTRLMGAFVTYLVKEKGIKNFFILSPNIT
                     IYDKLKDDFGNKGAEKYVFKGLPDISNNSVIITGEDYETKGIKASEDSIQINMFNIGK
                     INADVKTTTKDGRTLPPRIKRFRETIGDSYYNYLMNLKDLVIIMDESHRYRGERGMET
                     LNELNPLLGIELTATPIDASTNQRFKNVVFEYGLADALKDGKYIKNPAVVTRADTDFK
                     GMKDDEIEKLKLKDAIQMHRDTKISIENYIADNNLNPVKPTILVSCKDTTHAKSIFDY
                     VTSDEFYDGEFKEKTLQIDSTSKTDEQVEQLLTLEKLNNSIEIVIHVDMLKEGWDVSN
                     LYTIVPLRAANALTLIEQTIGRGLRLPFGGKRTGVEKVDMLSIMMHDKFQEVVDAAKD
                     KSSILYTIGKIQLNKSDLNSKKVSVESKTKDVVELEEEEKKITQIKDKDIRAKAETKH
                     KAKVVINAVIRNKISQKVIQTPNELTTPKVQEKVIEEIKESIKTTYPEMPEKEQKEIA
                     KDIETTYQTAVQEIIKNNILIPNIKVSISENKGIIKDFDLNTKSFSENILQQEFELIR
                     ETIYDHKQDKIKIQAQTLDLPEFSILELLKKKSEIDYDNNSDVMYKLCGQAVNAIKNS
                     LKKPETLGDTILMYRELIANKIYEQVKANITIEIVAKSKPEAEPYSLILPWNYSKEKD
                     DEILNFRETVSAKLLSSKVFNGFTKSCHDKYKFDSIPEKRFAEIIENSDDVIKWLRPA
                     SKQFNIWYNNNSDRYVPDFVAETADIIYLIEIKAENELDDKIVKLKEKAAINYCKNAT
                     EINKARKEKTWKYAIIPHDKTDTNMTFKKLVTEFGK"
     gene            complement(2995..4761)
                     /locus_tag="PK218_09735"
     CDS             complement(2995..4761)
                     /locus_tag="PK218_09735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPW98830.1"
                     /translation="MNSKYKKLELCWPGKDIEVKPEPRVLIEDPEKSYGEADSENMLI
                     HADNLLALKALEQNFSGKIKCIYIDPPYNTGNAFEYYDDGLEHSLWLDLMSQRINILK
                     KLLSKDGFFCCHIDDSEGAYLKVLLDEIFGRKNYLTTFYIRVRYPDKTLKQDMNFHKE
                     IEQVHIYRKEFGAKPNLNQSEITFDKFNFFISEKVEGKEIELGGKKVIVFQKGEYEIE
                     QRDEGTEFGLKEIWATGSILDGNSSGRFFRDYLTGRYETDGLGVLYKVYGIGDDRFDY
                     RYFTGPQRVGATKGKYFQGVPVDKLESETSMKETPINNFYDLAGSFGNCRSEGGVDFR
                     SGKKPEKLLNLIINHFSNEGDWILDSFLGSGSTMAVAHKMNRKWIGIELGNHANTHCL
                     PRIKAICEGADQTGISKEVNWKGGGGFKFYNLAPSLLNKDKYNQWVINPNYNADLLAA
                     AMARQEGFTYLPDESVLWKQGFATENHFIFTTTNTITPDVVEQILSEMKPDESLRICC
                     TAFIEGCNEISNRIEIKKIPEVLLGKCEFGKEDYCTDSNIINMPADHDKPAFTPSYVK
                     PDVKPEPANIKKDTPTQTKLLF"
     gene            complement(4795..5589)
                     /locus_tag="PK218_09740"
     CDS             complement(4795..5589)
                     /locus_tag="PK218_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98831.1"
                     /translation="MSRIVPFDSNYTTVRKFEKTAFYCLLFSAIAILYLWLIPKVEPL
                     ISFKISSTTIIDLIKVLSYVSMIGYIGLSSVAKYLFYSAEKVKRGDLIDNSFGTCYSE
                     SNSEEYYNNEEIGFGIKKLAVNTYESSFHTEQTLRKMLFRYALKMLLLSIPFLISIFS
                     IGGSDIVRLLFEISIPLIMLSQFFILIVYYTGVKSVNERFKIELTNIGNKQIALEDNA
                     KLLNPVFDYYNIKSWATTNLDSKIFDNHNDQISELWQKRKEKLKLV"
     gene            complement(5582..5962)
                     /locus_tag="PK218_09745"
     CDS             complement(5582..5962)
                     /locus_tag="PK218_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98832.1"
                     /translation="MKSKYPQFKAKHLSNETVTFIGDLMIKPELPIYCVSVEYRGTLS
                     PRVRVIEPKLVENPPHFYKNTECLCLYHSVDFKWDCRKLVAKEIMSWTIAWLYFYEAW
                     LQTGKWLGPESHHTPNTIKEDKDE"
     gene            complement(6000..6974)
                     /locus_tag="PK218_09750"
     CDS             complement(6000..6974)
                     /locus_tag="PK218_09750"
                     /inference="COORDINATES: protein motif:HMM:NF037736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98833.1"
                     /translation="MTLLESIKKLVDNVNITDRQEENIKNSLSNLETHLKNTENNLYV
                     KECFTSGSWERDTIIRPLNDVDLFAVLDFEEWKDEYGNKPNPQSVLTKIKNYLNDQND
                     YKDKVKQDRPCVTIHLSDKDFDVLPSFEISGGGYYIPNYDLKSWTSSYPKMLAKDLDD
                     THRLRDYKLKQIIRVVKYWNRDFNGKTIPSYHIEEIAINIFKMNTFTNFEEGIRKWFE
                     NVEYNMQSSKFKSNDEYESSLKKAKKVKDKLKEANKFYTDKNEVEAKKIWKDVFGKEF
                     PTVDEEEAKNISKSLTEGTLKYSSTAGISTAVGSSLSASKGFYGDEVL"
     gene            complement(6971..>7220)
                     /locus_tag="PK218_09755"
     CDS             complement(6971..>7220)
                     /locus_tag="PK218_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98834.1"
                     /translation="LIYTNVVSLLRKIDVGNKPKKKDNDTMTMKFLLHKDKEENGNPA
                     HKSKSVAALPCNRPIAATHMLFLPTRSLQIMNFKTFMI"
BASE COUNT         2099 a         1290 c         1056 g         2775 t
ORIGIN      
        1 taagcttgtg cataacggtc gagtgtatgt gccgtaaggg attgcggagc agtttcctgt
       61 ccaccgacac gaaagtttga gcgggctaca acgcttgaat acccactgaa acccttatgg
      121 catatacact ttgttggcaa ctggccgttc ttgtcattcg ttaatctgtc gttaattttt
      181 gctgcacttt ctttcagtcc gtgcgttgga gtgggacggg cttgaaggct cttttggcat
      241 ttttggtctg agggcaagcc ttgtgggaat gccaaatgtg cctgaaagcg aagggacggg
      301 tgcgtttgca tattattttc caaattcagt tactaatttt ttaaaagtca tgtttgtgtc
      361 tgtcttgtca tgcggaatta ttgcgtattt ccaagtcttt tcttttctcg ctttgtttat
      421 ttcggttgcg tttttgcaat aatttatagc tgctttttct ttcaacttga caatcttgtc
      481 gtcaagctcg ttttctgctt taatttctat taagtaaatt atgtctgctg tttcagcaac
      541 aaagtcaggt acatatctgt cagaattgtt attgtaccaa atattgaatt gctttgatgc
      601 tggtcttaac catttaataa catcgtctga attttcaatt atttcagcaa aacgtttttc
      661 aggtattgaa tcaaacttgt atttatcgtg gcatgatttt gtaaaaccat taaatacctt
      721 cgaagaaagt aattttgcac ttacagtttc tctgaaattc aatatttcat catctttctc
      781 ttttgaataa ttccaaggaa gaatcaaaga atatggttct gcttcgggct tagatttagc
      841 aacaatttca atagtgatat ttgcttttac ttgctcataa attttatttg caatcaattc
      901 acgatacatt aaaattgtat cgcctaaggt ttctggcttt ttgagtgaat tttttattgc
      961 atttacagcc tgtccgcaaa gtttatacat tacatcagaa ttgttgtcat agtcaatttc
     1021 tgacttcttt tttaataact ccaagatact aaattcaggc aagtcaagtg tttgagcttg
     1081 tatttttatt ttatcctgtt tgtggtcgta aattgtttct cggattaatt caaattcttg
     1141 ttgtaaaatg ttttctgaaa aagattttgt gtttaaatca aagtctttta taattccttt
     1201 gttttctgaa atagaaactt taatgtttgg aattaaaatg ttgtttttta taatttcctg
     1261 tactgccgtt tgataagtcg tttcaatatc tttggcaatt tctttttgtt ccttttcagg
     1321 catttcagga taggtcgttt taatgctttc ctttatttct tcgataactt tttcttgcac
     1381 cttaggagtt gttaattcat tcggtgtttg aattactttt tgagaaattt tatttcttat
     1441 tacagcattg ataacaacct ttgctttatg ctttgtttct gcttttgctc taatgtcctt
     1501 gtctttaatt tgtgtgattt tcttttcttc ttcttccagt tcaacaacat ctttagtttt
     1561 tgattcaacg gatacttttt tcgagtttaa atctgactta tttagttgga ttttaccaat
     1621 tgtataaaga attgaacttt tatcttttgc agcatcaaca acctcctgaa atttatcatg
     1681 catcataatt gaaagcatat ccactttttc aacgcctgtt ctttttccac caaatggcaa
     1741 acgcaaacct cttcctatgg tttgttcaat caaagttaag gcgtttgcag ctctcagagg
     1801 aacaattgta taaagatttg aaacgtccca accttctttg agcatatcaa catgaattac
     1861 aatttcaata gaattgttaa gcttttcaag agttagtaat tgttcaactt gttcgtctgt
     1921 tttacttgtt gaatctattt gcaatgtctt ttctttaaac tcaccatcgt aaaactcatc
     1981 agaagtaaca taatcaaaga ttgattttgc atgagtagtg tctttgcaag aaactaatat
     2041 tgttggtttt acagggttca aattattgtc agcaatataa ttttcaatag aaatttttgt
     2101 atctctgtgc atttgaatgg catctttcaa ttttagcttt tcaatttcgt catctttcat
     2161 tcctttaaaa tccgtgtctg cacgagttac aacagcagga ttttttatgt atttgccatc
     2221 ttttaaagcg tcagccaaac catattcaaa aacaacattt ttaaaacgct gatttgtact
     2281 tgcatctatg ggcgttgcag ttaattctat accaagcaat ggattaagtt catttagcgt
     2341 ttccatacct ctttcaccac gataacgatg tgattcgtcc atgatgatta caaggtcttt
     2401 taggttcatc aagtaattat aataactatc tccaattgtt tctctaaagc gttttattct
     2461 tgggggtaaa gttctaccgt cctttgttgt agtcttaaca tcagcgttta ttttgccaat
     2521 attgaacatg ttaatttgta tggaatcttc tgatgctttt atgccttttg tttcgtagtc
     2581 ttcgccagta ataataactg aattgtttga aatatcaggt agtcctttaa aaacatactt
     2641 ttctgctcct ttatttccaa aatcatcttt aagtttgtcg taaatagtaa tattgggaga
     2701 taaaataaaa aagtttttga ttcctttctc ttttaccaaa tatgttacaa aagcccccat
     2761 taatcgtgtc ttaccaatac cagtagcaat cgaaaaacaa acagaaggaa aaccacgttc
     2821 aaattcctca aataaagaac aatctttttt tattgattca attttctttt ctgtctttga
     2881 atttccaata aggtcggttt ttgcaattag cgaatccaat aactcaagtg attttttaag
     2941 aggttctctt aaactcatgg tattgcttat ataatcggtt gttttgctca tattctaaaa
     3001 caataattta gtttgcgtag gtgtgtcttt tttaatattt gccggttcgg gttttacatc
     3061 aggctttaca tacgaaggag taaaagctgg tttgtcatgg tctgctggca tattgataat
     3121 gttagaatct gtgcaataat cctcttttcc aaactcacat ttccctaaaa gtacttcggg
     3181 gattttcttg atttctatac ggttgcttat ttcattacaa ccttcaataa aggcagtaca
     3241 gcaaatacgc aaactttcat caggtttcat ttcgcttaga atctgttcaa ctacatctgg
     3301 tgttatggta tttgtggttg tgaaaataaa atgattttcg gttgcaaaac cttgtttcca
     3361 taaaacactt tcgtcaggca aatatgtaaa accttcctgt cttgccattg ctgctgcaag
     3421 caaatcagcg ttgtaattag gattaataac ccattgattg tatttgtctt tattcaataa
     3481 acttggggca agattataaa atttgaagcc accgccgcct ttccaattaa cttctttaga
     3541 tatgcctgtt tggtcagcac cttcgcaaat cgcctttatt cttggcaagc aatgagtatt
     3601 tgcatgatta cctaattcta tgcctatcca tttcctattc attttatgtg cgactgccat
     3661 tgtactccca gaacccaaaa aactatcaag aatccaatct ccttcatttg aaaaatgatt
     3721 aataattaga ttcaataatt tttctggttt tttcccactt ctaaaatcaa caccaccttc
     3781 acttctacaa tttccgaaac ttcctgctaa atcataaaaa ttattgatag gtgtttcctt
     3841 cattgaagtt tcactttcaa gtttatcaac tggaactcct tgaaaatact tgccttttgt
     3901 agcaccaact ctttgaggac ctgtaaaata tctataatca aatctgtcat caccaatacc
     3961 gtaaacttta taaagaacac ccaaaccatc agtttcatat cgtccagtta agtagtccct
     4021 aaaaaatcta cccgatgaat taccatccaa aatacttcct gttgcccaaa tttcttttaa
     4081 gccaaattct gtcccttcat ctctttgttc aatctcatat tcgccctttt gaaaaactat
     4141 tacctttttt cctccaagct caatttcttt accttcaact ttctctgaaa taaaaaaatt
     4201 gaatttatca aatgttatct ctgactggtt caaattaggt ttagcgccga attctttcct
     4261 gtatatatga acttgttcaa tttctttatg aaaattcata tcctgtttta atgttttgtc
     4321 aggatagcgg acacgaatat aaaatgtggt taaataattt tttcttccaa atatttcatc
     4381 aagaagtact ttaagatatg ctccttcgct atcatcaata tggcaacaaa aaaagccatc
     4441 ttttgaaagt aactttttta aaatattaat tcgttggctc attaaatcaa gccataaact
     4501 gtgttccaaa ccgtcatcat aatactcaaa tgcattacca gtattatatg gcgggtcaat
     4561 ataaatacat ttgattttgc ccgaaaaatt ttgttctaat gcttttaatg caagaagatt
     4621 gtcggcatgt atgagcatat tttcagaatc ggcttcgcca tacgattttt cagggtcttc
     4681 aatcaatact cttggttcgg gcttaacctc aatatctttt ccaggccaac aaagttcaag
     4741 ttttttatat ttactgttca ttcaaaatat ctgtttaaat aaaaatgcaa aatactaaac
     4801 taatttcagt ttctcttttc ttttctgcca tagttctgaa atttggtcgt tatgattatc
     4861 aaaaatttta ctgtctaaat tggtagtagc ccatgattta atgttgtaat aatcaaatac
     4921 aggattcagc aatttagcat tatcttctaa tgcaatttgt ttgttaccaa tatttgtaag
     4981 ttcaatttta aaccgttcat ttacgctctt tactcctgta taataaacta tcaatatgaa
     5041 aaactgtgaa agcataatca aaggaatgct tatttcaaaa agtaatctaa caatatcgct
     5101 accacctatt gaaaaaatag aaattagaaa cggtattgat aataagagca tttttaacgc
     5161 atatcgaaac aacatttttc gcaaagtctg ttctgtatgg aaactgcttt cataagtatt
     5221 tacagcaagt ttttttattc caaacccaat ttcctcatta ttatagtatt cctctgaatt
     5281 actttcagaa tagcatgttc cgaaagaatt atcaattaaa tcaccccgtt ttactttttc
     5341 agccgaataa aacaaatact ttgcaacact agaaagacca atatatccaa tcattgaaac
     5401 gtaacttagc acttttatta aatcaataat tgttgtagag ctaattttaa aagaaattag
     5461 tggctcaact ttaggtatta accataagta taaaattgca atcgcagaaa ataaaagaca
     5521 ataaaaggca gtcttttcaa attttcttac ggttgtgtaa ttgctatcaa atggaacgat
     5581 tctactcatc tttatcttct ttaattgtat ttggcgtatg atgtgattcg ggtccaagcc
     5641 attttcctgt ttgtaaccat gcttcgtaaa agtacagcca agcaatagtc catgacatta
     5701 tttcctttgc tacaagtttt cggcaatccc atttaaaatc aactgaatgg tacaaacaaa
     5761 ggcattctgt gtttttgtaa aaatgtggag gattttccac aagttttggt tctattacac
     5821 gaactctcgg tgataatgtt cctctatatt caacacttac acaataaatg ggtaactctg
     5881 gcttaatcat taaatctcca ataaacgtaa cagtttcatt agacaaatgc tttgctttaa
     5941 attgagggta cttcgatttc atcgccccaa tttgtgcaga tacatttggt ttatgcttat
     6001 taaagaactt catccccata aaaaccttta gatgctgata aactacttcc aactgcggtc
     6061 gaaattcctg ctgtgcttga atattttaat gtgccttcgg tcaatgattt tgaaatattt
     6121 tttgcttctt cttcatccac agttggaaac tcttttccaa aaacatcttt ccatatcttt
     6181 tttgcttcaa cttcgttttt gtcggtataa aatttattcg cttctttcaa tttatctttt
     6241 accttttttg cttttttcaa agaagactca tattcatcat ttgatttgaa tttacttgat
     6301 tgcatattat attcaacatt ttcaaaccat tttcttatcc cttcttcaaa gtttgtaaag
     6361 gtgttcatct tgaagatatt tattgcaatt tcctcaatgt ggtaagatgg aatagtttta
     6421 ccattaaaat cacggttcca gtattttaca actctgatta tttgtttcag tttataatct
     6481 cttaatctat gagtatcatc aaggtctttt gctaacattt ttggataaga agaagtccaa
     6541 gattttaaat catagttagg tatataatat ccaccaccag aaatttcaaa acttggtaaa
     6601 acatcaaaat ctttatcgct taaatgaata gtaacgcatg gtctatcttg ttttactttg
     6661 tctttgtaat cattctggtc attcaaatag tttttaattt ttgttaatac actttgagga
     6721 tttggtttat tgccgtattc atctttccat tcctcaaagt ccaaaacagc aaataaatca
     6781 acatcattca acggtcgaat tattgtatct ctttcccatg aaccacttgt aaaacactct
     6841 ttaacataaa gattattttc cgtgtttttc aaatgagttt ctaaattcga taatgaattt
     6901 ttaatatttt cttcttgtct gtcagttata ttaacattat cgacaagttt tttaatggat
     6961 tcaagtaatg tcatatcata aaagttttaa agttcattat ttgcagtgag cgggtcggca
     7021 aaaaaagcat gtgtgtggct gcgatgggac ggttgcaggg aagggcagcc acactcttgc
     7081 ttttgtgggc tgggtttcca ttttcttctt tgtctttatg cagcaaaaat ttcattgtca
     7141 ttgtgtcgtt gtcttttttc ttcggcttgt tgccaacatc tattttacgc aacaaagata
     7201 caacattcgt atatattaaa
//
LOCUS       DAOOBH010000110         7126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_12550_length_7126_cov_5.669778, whole genome shotgun sequence.
ACCESSION   DAOOBH010000110 DAOOBH010000000
VERSION     DAOOBH010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12550_length_7126_cov_5.669778"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /gene="hemN"
                     /locus_tag="PK218_09760"
     CDS             complement(<1..1034)
                     /gene="hemN"
                     /locus_tag="PK218_09760"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HPW98835.1"
                     /translation="MSISLIQKYNVPGPRYTSYPTVPYWDLDTFDASKWKQTYLQSFN
                     ESNTSEGISLYIHLPFCESLCTFCGCHKRITKRHEVESPYITAVLKEWDLYCQTFSEQ
                     PIIKELHLGGGTPTFFSPENLKTLINGIFSKAERAKDHEFSFEGHPNNTSREHLQTLF
                     ELGFRRVSYGVQDYSPIVQKAIHRDQSFHNVAKVTFWAKEIGYTSISHDLIYGLPFQT
                     LEDVIDTIEKTKSLSPDRLAFYSYAHVPWIKGNGQRGFKDEDLPKDDTKRLLYEIGKQ
                     LLEKKGYHEIGMDHFALTTDSMYQAFQDKKLHRNFMGYTSSKTQLMIGLGASSISDSW
                     YGFAQNEKSI"
     gene            complement(1140..2219)
                     /locus_tag="PK218_09765"
     CDS             complement(1140..2219)
                     /locus_tag="PK218_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW98836.1"
                     /translation="MKLKLNFLFVLALSVSSLFAQTTKKVAAKPKAPSAKKVETTKPV
                     ANTTDGIFAEINTSKGMIVVQLEYVKTPITVANFVSLAEGTNPEVKTELKGKPFYDGL
                     KFHRVIDNFMIQGGDPNGNGSGTAGYAFKDEFDPSLKHDKGGILSMANSGPKTNGSQF
                     FITHKETPWLDGKHTVFGHVVTGMDVVNAIKQDDLITSVKIVRKGAAAKKFDAPKIFG
                     TYMANKAEDDKKEAEMKNAIKEKALKEFANAKTTPSGLKYIVIKEGEGEMPTANTNVK
                     VHYTGMLLDGKIFDSSVQRGEPIDFPLNQVIKGWTEGVQLMKEGAKYKFYIPSNLAYG
                     ERGAGGVIPPNADLIFEIELLKINK"
     gene            complement(2240..3358)
                     /locus_tag="PK218_09770"
     CDS             complement(2240..3358)
                     /locus_tag="PK218_09770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW98837.1"
                     /translation="MNKTRFLLSFVALVMIFTACQNKEHNDLKDGLYAQLETSRGDIL
                     LELEYKKAPVTVANFVTLAEGKNPFAKEECKGKNLFDNISFHRVIKDFMIQTGDPLGN
                     GAGDAGYTFKDEFTDLRHDKPGVLSMANSGPNTNSCQFFVTQVPTPWLDGKHTVFGHI
                     VDSTNIDVVNAVTQGDKLNKVTIIRKGEAAKKFNAVKVFSDYFKSQSEENAKAKLLEE
                     ENKKEYAKKFEKVISDKLAYFDAIKEKATKLPSGVYYKIIQDAKGEKPKNGTQVSMKY
                     SGFLTDGTLFDTSEPEVAKQFGKFDPVRAHQNGYRALPYTVGTNQMIPGFAEGIKNMK
                     LGDKVVIFIPSKMAYGEQGAGGVIPPNADLIFEVELIK"
     gene            complement(3351..3932)
                     /gene="gldI"
                     /locus_tag="PK218_09775"
     CDS             complement(3351..3932)
                     /gene="gldI"
                     /locus_tag="PK218_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated peptidyl-prolyl
                     isomerase GldI"
                     /protein_id="HPW98838.1"
                     /translation="MKLNKTIIGLLLICLAFVSCKQPQARMPVSRSSGTFMKESIVRN
                     KKLIAGEEGKIDSIIKSNPNIKYFASNKGYWYHYEVKNDKDTLRPKKGDIAFFDYEVQ
                     DLKGNVIYSEIQTKPQVYKVDKQNIMMGLRHGIKLMHKNEKVVFLFPSHMAYGYHGDN
                     KNIGTNEPLLCIVTLNDFKPENTSTPTNILKNE"
     gene            complement(3934..4941)
                     /locus_tag="PK218_09780"
     CDS             complement(3934..4941)
                     /locus_tag="PK218_09780"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPW98839.1"
                     /translation="MNEIEISTVQELLSSPKKIAIIPHRSPDGDAMGSTLALYHFLLK
                     LNHQPVVIAPNDFPNFLAWLPGAETVLIYENDKTNCATILKEAQLVFTLDFNALHRTG
                     EMEHVLNKLTVPFIMVDHHQKPDTFAMVTYSNTAFGSTCEMIYNFISFLGKEKLIDKT
                     IGTCLYTGIMTDSGSFRFPSTTSNTHRVVANLIDLGVENSEIHNQVYDANSYNRLQLL
                     GRALQNMKVFPEHKTAYISLSQKELDEFHYVKGDTEGIVNYSLTIKGIHFAAIFIEHR
                     DENIIKISFRSQGSFDVNQFARDHFQGGGHINAAGGKSYDTLAHAINKFEKIIATLEI
                     E"
     gene            complement(5003..5374)
                     /gene="crcB"
                     /locus_tag="PK218_09785"
     CDS             complement(5003..5374)
                     /gene="crcB"
                     /locus_tag="PK218_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HPW98840.1"
                     /translation="MLKTILYIAIGGAIGSVLRYLTAVLVSKFWSSSFPLATFIVNVL
                     GCFLIGFFIGYLEKNQFTNDSLKWFLITGFCGGFTTFSAFGVENQFLFQNNHSFLAIS
                     YIGLSIFLGLLAVWLGLNLAK"
     gene            complement(5378..6193)
                     /gene="rluF"
                     /locus_tag="PK218_09790"
     CDS             complement(5378..6193)
                     /gene="rluF"
                     /locus_tag="PK218_09790"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HPW98841.1"
                     /translation="MELNQTRINKFLSESGFCSRREADKLLEQGRITINGIVPELGTK
                     VSTEDEIRVDGKLICENNEKQVYLAFHKPIGIECTTNQNVKNNIVDYINYPKRIFPIG
                     RLDKASEGLIFMTNDGDIVNKILRARNNHEKEYIVTVNRPITDRFIQKMGNGIPILDT
                     VTRKCKVEQVSKNIFRIILTQGLNRQIRRMCEFLDYEVVALKRTRIINISLDIPVGRY
                     RDLTDAEIKQLIELIEPSSKTEEASLPKSNPHIPRKTNSKAKIIPNKNSKRKQ"
     gene            6333..>7126
                     /locus_tag="PK218_09795"
     CDS             6333..>7126
                     /locus_tag="PK218_09795"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HPW98842.1"
                     /translation="MKIHGKLIIIGGAVDKGSFTERDLDQNAPNNLNFFEEGILKRII
                     KESKYKEQSRIEVITTASSIPREIGPEYVKAFSHLKATNVGVLYIEKREQASEPEVIE
                     RIKNADIVMFTGGDQLRLTSILGGTEMHTIILNKYKNEDFIYAGTSAGAAAASTNMIY
                     QGSSSEALLKGEVKITSGLALIDDVIIDTHFVKRGRIGRLFQAVVSNPKILGIGLGED
                     TGLLITNGKQMEAIGSGLVILVDGREIKDTNLTEVALGQPISINHLV"
BASE COUNT         2216 a         1203 c         1246 g         2461 t
ORIGIN      
        1 tcgattgatt tttcattttg agcaaaacca taccaactgt cgctaatgga agaagcaccc
       61 aaaccaatca ttaactgagt tttggaagaa gtgtagccca taaagttgcg atgcagtttt
      121 ttatcttgaa aggcttgata catactgtcg gtagttagtg caaaatggtc cattccaatt
      181 tcgtgatagc cttttttttc taataattgc tttcctattt cgtagagtag gcgtttggta
      241 tcgtctttgg gtaggtcttc gtctttaaat ccacgttgtc cgttgccttt aatccatggt
      301 acatgagcat agctataaaa agctaatcta tcgggcgata gtgactttgt tttttcaata
      361 gtgtcaataa catcttctaa tgtttgaaac ggtagcccat aaatcaaatc gtgtgaaatg
      421 gaagtatagc caatttcttt tgcccaaaac gtaacttttg ccacattgtg aaacgattgg
      481 tcgcgatgga tggctttttg aacgattgga ctgtaatcct gcacaccata actcacacgt
      541 ctaaaaccaa gttcgaatag ggtttgcagg tgttcccgag aagtattgtt tggatgacct
      601 tcaaaactaa attcgtggtc tttggctctt tcggctttac taaatatgcc gtttattaat
      661 gtttttaaat tttctggtga aaagaaagtt ggggttccac caccaagatg gagttcttta
      721 attattggtt gctcagaaaa ggtttgacaa tacaaatccc attcttttaa aacagccgtt
      781 atatacggac tttcaacttc atgacgtttg gtaattcgtt tatgacatcc acaaaaggtg
      841 cacaggcttt cgcaaaaagg caaatgaatg tatagactaa taccttcgga agtgttactt
      901 tcattaaaag attgaagata ggtttgtttc cattttgagg catcaaaagt atctaaatcc
      961 caataaggaa ccgttggata actagtatat cttggaccag gaacattgta tttttgaatg
     1021 agcgaaatag acatgaatat ccaaattaat acttcaaaag taactagaga agtgatatta
     1081 taaaatgata attgtcatgt ataaaaaaag tcccaaaata ttcgggactt tcttaatact
     1141 tatttattga tttttaataa ttcaatttcg aatattaaat cggcatttgg aggaataact
     1201 ccaccagcac cgcgttcgcc ataggctaaa ttagatggaa tataaaattt atattttgcg
     1261 ccttctttca tcagttgaac accttcagtc caacctttaa tcacttgatt taaaggaaaa
     1321 tctattggtt cgcctcgctg aacactacta tcaaaaattt taccgtctaa taacattcct
     1381 gtgtaatgca cttttacatt tgtattggct gttggcattt ctccttcacc ttctttaatg
     1441 acaatatatt ttaatccgct aggggttgtt ttagcatttg caaattcttt taaagctttt
     1501 tcttttatcg catttttcat ttcggcttct tttttgtcat cttctgcttt gtttgccata
     1561 tatgtaccaa agatttttgg agcatcaaac ttttttgcag ctgcaccttt gcgaacaatc
     1621 tttacagagg taattaaatc gtcttgtttg atggcgttta caacatccat tccggttact
     1681 acatgtccaa aaacggtatg ttttccatcc aaccaaggcg tttctttgtg agtaataaaa
     1741 aactgagaac cattagtttt tggtcctgag tttgccatag atagtattcc gcctttgtcg
     1801 tgctttaagg atgggtcaaa ctcatcttta aacgcataac ccgccgttcc agaaccgttg
     1861 ccatttggat caccaccttg aatcataaag ttgtctataa ctctgtggaa ttttaaacca
     1921 tcataaaaag gttttccttt tagttcggtt tttacttcag gattggttcc ctctgccaat
     1981 gaaacgaagt tagccaccgt aataggtgtt tttacatatt ccaattgaac tacaatcatt
     2041 cctttagacg tgttgatttc agcaaagata ccatcagttg tattggctac tggttttgtt
     2101 gtttcaactt tttttgctga aggagctttc ggtttagcag ctactttttt ggtggtttgt
     2161 gcaaaaagcg acgaaacgct taatgctaaa acaaacaaga aatttaattt tagtttcatt
     2221 ttactattct atttattatt tatttaatta attcaacttc aaagattaaa tcggcgtttg
     2281 gcggaataac accaccagca ccttgttctc cataagccat ttttgacgga atgaagatta
     2341 cgactttgtc tcccaatttc atatttttta tgccttctgc aaaaccagga atcatttgat
     2401 ttgtaccaac ggtataaggt aacgctctgt atccgttttg atgcgctctt actggatcaa
     2461 attttccaaa ctgtttggcg acttcaggtt cacttgtgtc aaaaagtgta ccatcagtaa
     2521 gaaaaccact gtatttcatt gatacttgag taccgttttt aggtttttct ccttttgcat
     2581 cttgaatgat tttataatac acaccagacg gtagcttggt agctttttct ttgattgcat
     2641 caaagtaagc gagtttgtcc gatattactt tttcgaattt tttagcatat tcttttttat
     2701 tttcttcttc caaaagtttt gcttttgcat tttcttccga ttggcttttg aaataatcgc
     2761 taaatacttt aacggcatta aatttcttag cagcttctcc tttgcgaatg atagtaactt
     2821 tattgagttt atcgccttgc gtaacagcat taacgacatc aatattagta gaatcgacaa
     2881 tatgtccaaa aacagtgtgt tttccatcaa gccatggcgt aggaacttgg gtaacaaaaa
     2941 attgacaact gttggtgttt ggtccagagt ttgccataga tagcacacca ggtttgtcgt
     3001 gtctcaaatc ggtaaattcg tctttaaaag tataaccagc atcgccagca ccattaccta
     3061 agggatcgcc tgtttgaatc atgaagtctt taataactct gtgaaacgaa atgttgtcga
     3121 aaagattttt tcctttgcat tcttctttag caaaagggtt ttttccttcg gctaaggtta
     3181 caaaattggc tacagtaact ggtgcttttt tatattctaa ttctaataat atgtctcctc
     3241 tagaagtttc taattgagca tatagtccgt cttttaaatc gttatgttcc ttattttgac
     3301 aggcagtaaa tatcattaca agcgcaacga aagataaaag aaatctagtt ttattcattt
     3361 tttaaaatgt tggttggagt tgaagtattt tcgggtttaa agtcgtttaa agtaacaatg
     3421 caaagtaaag gttcgttggt gccaatgttt ttattatcgc catgatatcc ataagccatg
     3481 tgtgaaggaa ataggaaaac tactttttcg tttttgtgca tcaatttaat tccatgacgc
     3541 aaacccatca tgatgttttg tttgtccact ttatacactt gtggtttggt ttgaatttcg
     3601 gaataaatta cgtttccctt taaatcttgc acttcatagt caaagaaagc aatgtctcct
     3661 ttttttggac gaagagtatc tttgtcattt tttacctcat aatgatacca gtaacctttg
     3721 ttggaagcaa aatatttgat gtttggatta cttttaataa tcgaatcaat ttttccttct
     3781 tcgccagcaa tcaatttttt atttcgaaca atagattctt tcataaaagt tccagaagag
     3841 cgagaaacag gcatgcgagc ttgtggttgt ttgcagctta caaacgctag gcaaatcaat
     3901 aacaatccaa taatggtttt atttaatttc attttattca atttcaagag tggcaattat
     3961 tttttcaaat ttattgatag cgtgtgctaa agtgtcatac gattttccac cagcagcatt
     4021 gatgtgtcca ccgccttgaa aatgatctct ggcaaactga ttgacatcaa aacttccttg
     4081 cgaacggaac gaaatcttaa tgatattctc gtcgcgatgt tctataaaga tagctgcaaa
     4141 atggatgcct tttatggtta gactataatt gacaatgcct tcggtatcac cttttacata
     4201 gtgaaactca tctaattctt tttgagaaag tgagatatag gctgttttat gttctgggaa
     4261 aactttcatg ttttgcaaag ctctacccaa taactgcaaa cgattgtatg aattagcatc
     4321 atatacttga ttgtgaattt cactattttc aacgcctaaa tcaattaaat ttgccacaac
     4381 tctatgtgta ttgctagtgg tagaaggaaa acggaatgaa cccgaatcag tcataattcc
     4441 agtataaaga caagttccaa tggttttatc aatcaacttt tctttgccta aaaaagaaat
     4501 gaaattataa atcatttcgc aggtagaacc aaaagcagta tttgaataag taaccattgc
     4561 aaaggtatca ggtttttgat gatgatcaac catgataaat ggaacagtaa gcttgtttaa
     4621 aacatgttcc atttcacctg ttctatgcaa agcattaaaa tctaatgtaa agactaactg
     4681 tgcttctttt agaatagtag cacaattggt tttgtcattt tcataaatta aaacagtttc
     4741 tgcgccaggc aaccaagcta aaaagtttgg gaaatcattt ggcgcaataa caacgggttg
     4801 atggtttaat ttcagcagaa aatgatataa agctaatgta gaacccattg catcaccatc
     4861 tggacttcga tgaggaataa tagctatttt ttttggactt gaaagtaatt cttgtactgt
     4921 cgaaatttcg atttcattca tagcttgcga aaatactaaa aaatgaaaca aaccgcataa
     4981 agattagcat agaatttaga ttttatttag ctaaatttaa accaagccaa acggccaaaa
     5041 gacctaagaa tatgctcaat ccgatgtagc ttatggctag aaaggaatga ttgttttgaa
     5101 atagaaattg attttctaca ccaaaagcgg aaaaagtggt aaagccacca caaaatcctg
     5161 taattaaaaa ccattttaac gaatcattgg taaattggtt tttttctaaa taaccaataa
     5221 aaaagccaat aagaaaacaa ccaagtacat ttacgataaa agtagccaat ggaaaagagc
     5281 ttgaccaaaa ctttgaaacc aaaacggcag ttaaatagcg aagaacactt ccgatggcac
     5341 caccaatagc gatgtataaa atagttttta acatggatta ctgttttctt ttggaatttt
     5401 tattgggaat gattttagct ttcgaattgg tttttctagg aatatgtgga tttgattttg
     5461 gcaaacttgc ttcttctgtt ttactcgaag gttcaatcaa ttcaatcaat tgtttgattt
     5521 cagcgtcggt taaatctcta taacgaccca caggaatgtc tagtgaaata ttgataattc
     5581 gggttctttt taatgctaca acttcataat caagaaattc acacattcta cgaatttgac
     5641 ggttcaatcc ttgtgtcaat ataatacgga aaatgttttt gctgacttgt tctactttac
     5701 attttcgggt aaccgtatca agtattggaa ttccatttcc cattttttga ataaatcggt
     5761 cagtaattgg gcgattaacg gttacgatat attctttttc gtgattgttt cgagcacgaa
     5821 gaattttatt gacaatatcg ccatcattgg tcatgaatat caaaccttcc gaagctttgt
     5881 ctaaacgacc aattgggaat attcttttag gataattgat gtaatctaca atgttgtttt
     5941 ttacattttg attggtggta cattcaattc caataggctt atggaaagcc aaatagactt
     6001 gtttttcatt attttcacaa atcagttttc cgtccactcg aatttcatct tcagtagaaa
     6061 cttttgtacc cagttcagga acgattccgt ttatggtaat tctaccttgt tccaataatt
     6121 tatcggcttc acgacgagag caaaaccctg attcggatag aaatttattt attctggttt
     6181 ggtttaattc cattgtacaa atataaataa tttaaggata atgaaatttt aatagtggag
     6241 taaaaaaaca tcttaataac tacgttaata aataggaatc aaatttgaaa aaatatcgat
     6301 gttactatta agtttttgtt atttttacag ccatgaaaat acacggaaaa cttattataa
     6361 tcggtggagc tgttgataaa ggcagtttta ctgagcgcga tttggatcag aatgctccaa
     6421 acaacttaaa tttctttgaa gaaggaattt taaaaagaat catcaaagaa tctaaatata
     6481 aagagcaatc aagaattgag gttatcacaa cagcgtctag tattccaaga gaaattggtc
     6541 ctgagtatgt aaaggcattt agtcacttaa aggcgacgaa tgttggtgtg ctttatatag
     6601 aaaaaagaga acaagccagc gaacccgaag taattgaacg tatcaaaaac gctgatattg
     6661 ttatgtttac tggtggcgac caattgcgtt tgacttcaat acttggagga acagaaatgc
     6721 acaccatcat tttaaataag tataaaaatg aagatttcat ctatgcggga acttcagctg
     6781 gtgcagcggc agcttctact aatatgattt atcaaggaag tagctcggaa gctttgttga
     6841 aaggtgaagt aaaaattaca tcagggttag ctttgattga tgatgtaatt atagacactc
     6901 attttgttaa acgcggacgt atcggacgct tgtttcaagc agtagttagt aatcctaaaa
     6961 tactcggaat tggtcttgga gaagatactg gtttgttgat taccaatgga aaacaaatgg
     7021 aagctatagg ttctggttta gtaattttag tggatggaag agaaattaaa gatacaaatc
     7081 taacagaagt tgctttagga caaccgattt cgataaacca tttagt
//
LOCUS       DAOOBH010000111         7110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_12586_length_7110_cov_7.366690, whole genome shotgun sequence.
ACCESSION   DAOOBH010000111 DAOOBH010000000
VERSION     DAOOBH010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12586_length_7110_cov_7.366690"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /gene="rpsI"
                     /locus_tag="PK218_09800"
     CDS             complement(<1..327)
                     /gene="rpsI"
                     /locus_tag="PK218_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPW98843.1"
                     /translation="MGVIHKIGRRKCAVARVYVSEGTGKITVNKREFETYFPTATLQY
                     KVMQPMSLTENASNFDVKVNVYGGGSTGQAEAVRMAIARAMCEVEAENRAILKPEGLL
                     TRDPRMV"
     gene            complement(327..782)
                     /gene="rplM"
                     /locus_tag="PK218_09805"
     CDS             complement(327..782)
                     /gene="rplM"
                     /locus_tag="PK218_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPW98844.1"
                     /translation="MNTLSYKTVSANKATVDKQWIVVDAEGHNLGRFASKVAMLLRGK
                     YKPSYTPHVDCGDNVIVINAEKINLTGNKLDDKTYIRHTGYPGGQRSLTAKVMQQKNP
                     ALLVEKAVKGMLPKNKLGAELFRNLNVNVGSEHKHAAQQPKTVNLNDLK"
     gene            958..1980
                     /locus_tag="PK218_09810"
     CDS             958..1980
                     /locus_tag="PK218_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW98845.1"
                     /translation="MKSKATLKQIAKELGVSVSTVSKALNGSLEISEPTKQRVQEYAK
                     LKNYKPNVIGLNLKNRSTKTIGVIIPDILNSFFAKVFTGIEKVADDRGYKLITCISNE
                     SYQKEINALEMLSNGTIDGFILAIADETQKLHQFNHFKEVLNEGIPIVMFDRISDEIN
                     CDKVIVDDFESAINATNHLIERGCRKIALLSAIDNLSVGKLRAEGFYKAIKNHGHQVD
                     ENLVILTNTNEEFNDKIGGFFIKNKPDGVFALDEHASVTAMKLGIQNGYKVPNDLQII
                     GFADGLWSRRMTPSMSTVSQHAPEIGEVAASLLIDRLENKEEKTLIITRVIKTELRKR
                     DSTKKV"
     gene            2044..2577
                     /locus_tag="PK218_09815"
     CDS             2044..2577
                     /locus_tag="PK218_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPW98846.1"
                     /translation="MNSTNRLFVVFLWVISSAVFSQEKLDVFNVARNGTTEQLLSIVK
                     SNPKEINNINSDGYSPLILACYRGNNEVAKMLIENGADINYKSALGSPLMACVVKNNN
                     EIAKILISKGANVNITDANGMTAILYATMFKNYEITRLLVKNKADIQAKDNRGNSALD
                     YAILADDDTLIEILKTK"
     gene            2580..3353
                     /locus_tag="PK218_09820"
     CDS             2580..3353
                     /locus_tag="PK218_09820"
                     /inference="COORDINATES: protein motif:HMM:NF015318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98847.1"
                     /translation="MKKITLTFFIYMLSMGLFAQTYSTGMVTLNAIGGGNLGMSVQID
                     VTSDMVTLTLIGPSTGWLGIGFNNTSMADSGDVVIFDGTNLTDRNFVGFGTEPLLDGQ
                     QDWTVTSNTVDAGIRTVVGRRVRDTGDADDYVFSATAQSLNLVYARKISDFSIGYHGG
                     GSCGTVMSSFTLGSKEFDLDSFKLYPNPATNNYTSIELPQNINNGIVKIYDTLGRLVM
                     KQEVSTDASLINTSNLKSGSYLFVLRTEYGNATKNMIIN"
     gene            complement(3493..6348)
                     /gene="polA"
                     /locus_tag="PK218_09825"
     CDS             complement(3493..6348)
                     /gene="polA"
                     /locus_tag="PK218_09825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPW98848.1"
                     /translation="MMSQKRLYLLDAYALIFRGYFAFIKNPRINSKGMDTSAIMGFMN
                     ALMDVIKREKPDHLAVAFDKGGSDLRNEIFPEYKAHRDETPEAIKIAVPYIQELLKAM
                     HIPIMEKAGFEADDLIGTLAKQAEKEGFQVFMVTPDKDFAQLVSENIFMYKPARMGND
                     IEIWGIPEVQAKFEIERPEQVIDFLGMMGDSADNIPGLPGVGEKTAKKFLAEYGSLEN
                     LLANTHELKGAMKEKIEANKELGLLSKKLATILLDCPVTFDAKDFELSKPDVEKTDAL
                     FQELEFRQMKAQFDKYFGTGKEYDEIDTNGNSSETSQIVKKATAKKTNEDQMDLFGFS
                     DDDANENAFHSGYSTIENTEHFYQIVQGDLPVKLLLQNLIKQTSVCFDTETTGIDALN
                     AELVGMSFSYEKGKAFYVPFPENRDEAQILIEKFKPFFENESIEKIGQNVKYDLKILS
                     NYGVQIKGKLFDTMIAHYLINPDMRHNMDVLSETYLKYSPKSIEDLIGKKGKNQKSMR
                     DVALEEIKEYAAEDADITLQLKQHFQPILEKVGTKKLFDEIEIPLVPVLAAMELEGIN
                     LDVPFLKEMSIEMAKESGALEQKIYETAGEKFNLASPKQLGDILFDKMKIGGPKQKKT
                     KTGQYATGEEVLSYLAKDNEIVRDILEWRQMVKLQSTYIDALPNQVDAKTGRVHTDYM
                     QTVAATGRLSSNNPNLQNIPIRTERGRLIRKAFIARDENYTLLSADYSQIELRIIAAL
                     SGEDNMIKAFQNHEDIHKSTAAKVFNVPLDEVTKEQRSNAKTVNFGIIYGVSAFGLSN
                     QTDLSRSESADLIDAYYKTYPKLKSYMSNQVDFARENGFVETVLGRRRYLKDINSANA
                     VVRGAAERNAVNAPIQGSAADIIKIAMINIHKKLTSENWKSKMLLQVHDELVFDVHNS
                     ELEKIQPMIKYEMEHAFKLDVPLEVEMGMGKNWLEAH"
     gene            complement(6450..>7110)
                     /locus_tag="PK218_09830"
     CDS             complement(6450..>7110)
                     /locus_tag="PK218_09830"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HPW98849.1"
                     /translation="NTSGNYNTAIGRDALNTNSTGDGLTGLGRGTDVSVDGLTNATAI
                     GYNAVVNASNKIRIGNAGVTVIEGQVAYSFPSDARFKFNVQDNVPGLLFIKKLKPITY
                     QFDTEKFDSYVGKNNAENNTESFQKSKEIIHSGFLAQDIEKVCQELKYDFDGLHIPDT
                     TNQTDHYSLAYSQFIMPMVKAIQEQQEIIEQQNNELEQLKSEIKQLKTLEERIKLLEN
                     K"
BASE COUNT         2207 a         1190 c         1159 g         2554 t
ORIGIN      
        1 taccattctt ggatctcttg ttaataatcc ttctggtttt aagatagctc tgttttcagc
       61 ttccacttca cacattgctc ttgcaatagc cattctcacg gcttctgctt gtcctgttga
      121 accaccacca taaacattta cttttacgtc aaagtttgat gcgttttctg tcaatgacat
      181 tggttgcatt actttgtact gtaaagtagc tgttgggaag taagtttcaa attctctttt
      241 gtttacggta atttttcctg ttccttccga aacataaaca cgtgccacag cacattttct
      301 tctaccgatt ttgtgaataa ctcccattac tttaagtcgt taaggtttac ggtttttggt
      361 tgttgagcag cgtgtttgtg ctcagaacca acatttacat tcaaattgcg gaataattct
      421 gcacctaatt tgtttttagg caacattcct tttacagcct tttctactaa taatgcaggg
      481 tttttttgtt gcattacttt agcagtcaag cttctttgtc ctcctggata acctgtgtgt
      541 ctgatgtacg ttttgtcatc taacttgtta ccagtcaggt tgattttttc tgcgttgata
      601 acaattacgt tatctccaca atcaacgtgc ggtgtataac ttggcttgta cttgcctctt
      661 aaaagcattg caacttttga agcaaatcta cctaagttat gaccttcagc atctacaaca
      721 atccactgtt tatcaactgt ggctttgttt gctgaaactg tcttgtagct taatgtgttc
      781 acaataattt attttaatta aacattccat tcccaattac gggagtgcaa aagtacaatt
      841 atttattaat tatacaaaca ctcctaaaaa atcttttttg tttgcatttc agctatttat
      901 atattattac aatttgtgaa aaaatatttt cctattttta cacaataact atatataatg
      961 aaatcaaaag ccacgttaaa acaaatcgca aaagagctag gagtttccgt ttctactgtg
     1021 tctaaagcac taaatggcag tctcgaaatc agtgagccta caaaacaacg tgttcaagag
     1081 tatgctaaac ttaagaatta taaaccgaat gttattggtt taaaccttaa aaacagaagt
     1141 actaaaacta ttggtgttat tattcctgat attttaaatt ctttctttgc aaaagtattt
     1201 acaggtatag aaaaagttgc tgatgaccgt ggatacaaat tgattacttg catttctaac
     1261 gaatcctatc aaaaggaaat taatgcgtta gaaatgttaa gtaatggaac tattgatggt
     1321 tttattcttg ccattgctga cgaaacacaa aagcttcacc agtttaatca ttttaaagaa
     1381 gttttaaatg aaggtattcc aatagttatg tttgatagaa tttctgatga aataaattgt
     1441 gataaagtaa ttgttgatga ttttgagtct gccattaatg cgacgaatca tttaatagaa
     1501 agaggttgta gaaaaattgc tttgctatcg gctattgata atttgagtgt tggaaaactt
     1561 agagccgaag gtttttataa agccataaaa aatcatggtc atcaagtgga tgaaaattta
     1621 gttattttga ccaatactaa tgaagaattt aatgataaaa ttggcgggtt ttttatcaaa
     1681 aacaaacctg atggagtctt tgcgttagat gaacatgctt cggttaccgc aatgaaactt
     1741 ggtattcaaa acggatataa agttccaaat gatttgcaaa ttattggttt tgccgatggt
     1801 ctttggtcgc gaagaatgac tccaagtatg tcaaccgtta gtcagcacgc accagagata
     1861 ggcgaagttg cagcaagttt acttattgat agattagaaa ataaagaaga aaaaactcta
     1921 ataattacaa gagttattaa aacagagctt cgaaaaagag attcgactaa gaaagtttaa
     1981 aaatttatat taaattgtaa cgcaactgtt tttgtttttt tgtatctttt aataaaatat
     2041 aacatgaatt ctactaatag attgtttgtt gtttttcttt gggtcatttc aagtgctgtt
     2101 ttttctcaag aaaaactaga tgtttttaat gtagcacgga acggaacgac agaacagcta
     2161 ctttcaattg taaagagcaa tccaaaagaa ataaacaaca tcaatagtga tgggtattct
     2221 cctttgattt tagcatgcta tcgtggaaat aatgaagtag caaaaatgtt aattgaaaac
     2281 ggcgcagata taaattataa aagcgcattg ggttctcctt taatggcttg tgttgtaaaa
     2341 aacaataatg aaatagctaa gattttaatt agtaaaggcg caaatgtaaa tatcacagat
     2401 gcaaatggaa tgactgccat attatatgct acgatgttta aaaattatga aatcacccgt
     2461 ttgttggtaa aaaataaagc agatattcaa gcaaaagaca acagaggaaa ctcagcttta
     2521 gattatgcaa tcttagcaga tgatgataca ttaatcgaaa tattaaaaac caaataacta
     2581 tgaaaaaaat tactttaact ttctttattt acatgttgtc aatgggtttg tttgctcaaa
     2641 cttattcaac aggcatggtg acattaaacg caattggagg tggcaactta ggaatgtcgg
     2701 ttcaaattga cgtgacctca gatatggtaa ctctaacttt aatcggaccg tcaacaggtt
     2761 ggcttggaat tggttttaat aatacttcaa tggccgactc tggcgatgta gttatttttg
     2821 acggcactaa tcttactgat agaaattttg taggttttgg taccgaacct cttttggatg
     2881 gacaacaaga ttggacagtt acttcaaata cagtagatgc tggaataaga accgttgttg
     2941 gtagaagagt aagagatact ggcgatgcag atgattatgt ttttagtgca accgctcagt
     3001 ctttaaactt agtgtatgct agaaaaattt cggatttttc aataggttat catggtggtg
     3061 gaagttgtgg gacagttatg tcttctttta cattaggtag caaggagttc gatctagatt
     3121 cattcaaact ttatccgaat cctgcaacca ataattacac ttcaattgaa ttacctcaaa
     3181 atattaacaa cggaatagtt aaaatatatg acacattagg cagattggtg atgaaacaag
     3241 aagttagtac tgatgccagt ttaattaata cttccaattt aaaatcaggt agttatttgt
     3301 ttgtattaag aacagagtat ggtaatgcta ctaaaaatat gattatcaat taattcagta
     3361 ctaattaaaa taaaaggata gctatttata aactatcctt tttttatttg taatgatatt
     3421 aaggatttaa aatgtcttaa attattgtct aaaaaactaa tatcggaact ctaacagcta
     3481 aaattcattt tcttaatgcg cttccaacca gttttttccc attcccattt caacctccaa
     3541 aggaacatct aatttgaaag catgttccat ttcatattta atcatgggtt gaattttctc
     3601 taattcacta ttatgaacat caaaaaccaa ttcatcatgc acttgtaata acatttttga
     3661 tttccagttt tcagaagtca attttttatg aatgttaatc atcgcaattt taataatatc
     3721 agctgccgaa ccttgtattg gagcatttac tgcatttcgt tcagctgcgc cacgaacaac
     3781 ggcgttggct gaatttatgt cttttaaata acgacgtctt cctaaaacgg tttcaacaaa
     3841 accattttct ctggcaaaat caacctgatt tgacatgtat gattttaatt ttggatacgt
     3901 tttataatac gcatcaatca aatcggcact ttcagaacgt gataaatcgg tttgattgga
     3961 aagtccaaaa gccgaaacgc cataaatgat accaaagttc actgttttgg cattacttcg
     4021 ttgttctttg gttacttcgt ctaatggtac attaaacact tttgccgcag ttgatttatg
     4081 aatatcttca tgattttgaa atgctttaat catgttgtcc tctccagaaa gtgctgcaat
     4141 aattcgcaat tctatttgag aataatcggc agatagtaac gtataatttt catctcgtgc
     4201 gataaaggct tttctgatta atcgaccacg ttcagttcga ataggaatat tctgcaaatt
     4261 tggattattt gaacttaatc gtcctgttgc ggcaaccgtt tgcatataat cagtatgaac
     4321 tcttcccgtt tttgcatcga cttgatttgg taatgcatcg atataggtac tttgcaattt
     4381 taccatttgt cgccattcaa gaatatcgcg cacaatttcg ttgtcttttg ccaagtaact
     4441 cagtacttct tcgccagttg cgtattgtcc ggttttggtt ttcttttgtt ttggtccacc
     4501 aattttcatt ttatcaaaca aaatatcgcc tagttgtttt ggtgaagcta aattgaattt
     4561 ctcaccagct gtttcatata ttttttgttc caaagctcca ctttcttttg ccatttcaat
     4621 cgacatttct tttagaaatg gaacatcaag attgatgcct tctaattcca ttgcagctaa
     4681 aactggaact aaaggaattt cgatttcatc aaataatttt tttgtgccaa ctttttctaa
     4741 aataggttga aaatgttgtt tcaattgcaa ggtaatatca gcgtcttctg ctgcatattc
     4801 tttgatttct tctaaagcta catcgcgcat cgatttttga tttttacctt tttttccaat
     4861 taaatcttct atagattttg gtgaatattt taaatacgtt tcactcaaaa cgtccatatt
     4921 gtgacgcata tctggattta tcaaataatg cgcaatcatg gtgtcaaata gttttccttt
     4981 gatttgaaca ccataattag aaagaatttt caagtcatat tttacgtttt gcccaatttt
     5041 ttcaatggat tcattttcaa aaaaaggttt gaatttttcg attaaaattt gtgcttcatc
     5101 acggttttca ggaaacggaa cgtaaaacgc ctttcctttt tcataggaga acgacatgcc
     5161 tactaactct gcattcaagg catcaattcc agtagtttcg gtatcaaaac acaccgaagt
     5221 ttgctttatc agattttgta ataataattt aaccggtaaa tcaccttgaa ctatttgata
     5281 aaaatgttcg gtgttttcta tagtggaata acctgagtga aatgcgtttt cattggcgtc
     5341 atcatctgaa aaaccaaaca aatccatttg atcttcatta gttttttttg ctgtagcttt
     5401 tttaactatt tgtgaagttt cactagaatt tccattggtg tcaatttcat catattcttt
     5461 tcctgttcca aaatatttat caaactgcgc tttcatttgg cgaaactcta gttcttgaaa
     5521 caaagcatcg gttttttcga catcaggttt tgacaattca aaatcttttg catcaaaagt
     5581 caccggacaa tcgagtagaa tagtagctaa tttttttgaa agtaatccca attctttatt
     5641 agcttcaatt ttttctttca ttgcgccttt taattcatgc gtattagcta aaagattttc
     5701 taacgaaccg tattcggcca aaaatttctt ggctgttttt tcaccaacac caggcaaacc
     5761 aggtatgtta tcggcagaat cgcccatcat gccaaggaaa tcaatcactt gttctggtct
     5821 ttctatttca aatttagctt gcacttctgg aattccccaa atttcaatat cgttacccat
     5881 tcgagccggt ttatacatga aaatattttc ggaaactagt tgtgcgaaat ctttatcggg
     5941 tgtaaccata aaaacttgaa aaccttcttt ttcagcttgt tttgccaagg ttccaatcaa
     6001 atcatcagct tcaaatcctg ctttttccat tataggaata tgcattgctt taagcaattc
     6061 ttgaatatag ggaacagcaa tttttatagc ttcgggagtt tcatcacgat gagctttata
     6121 ttctggaaag atttcgtttc tcaaatcact tccgcctttg tcaaatgcca ctgctaaatg
     6181 atctggtttt tcacgtttaa tgacatccat taaagcattc ataaaaccca ttatggcaga
     6241 tgtgtccata cctttagaat tgatacgagg atttttaata aaggcaaaat aaccgcgaaa
     6301 aattagggcg taggcatcaa gaaggtaaag gcgtttttgt gacattataa tttattttct
     6361 tcaaatgtaa taaacttaaa tagcttgaaa aagaaaagga gttccaaaat tgtgaactcc
     6421 tttgctaaaa ctaaaactcc tactaacttt tatttgtttt caagcaattt tattctttct
     6481 tctaaggttt ttaattgttt aatttctgat tttaattgtt caagttcatt gttttgttgt
     6541 tcaattattt cttgttgttc ttgaatggct tttaccattg gcataataaa ttgagaataa
     6601 gcaaggctat aatgatctgt ttgatttgta gtgtcgggaa tatgtaatcc atcaaaatca
     6661 tactttagtt cttgacaaac cttctcgata tcttgtgcta aaaatccgga atgaataatt
     6721 tctttcgatt tttgaaatga ttcggtattg ttttcagcat tgttttttcc aacataagaa
     6781 tcaaattttt cggtgtcgaa ttgataggta attggtttta attttttgat aaaaagtaat
     6841 ccaggtacat tatcttgtac gttaaattta aaacgtgcgt ctgaaggaaa agagtaagca
     6901 acttgtcctt caattacagt tactccagca ttaccaatgc gtattttatt gcttgcattt
     6961 acaaccgcat tataaccaat agccgttgca ttggtaagac catcaactga aacatctgtt
     7021 cctcttccaa gaccagttaa tccatcacca gtgctattgg tgtttaatgc atcacggcca
     7081 atggcggtat tataatttcc gcttgtgttt
//
LOCUS       DAOOBH010000112         7104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_12601_length_7104_cov_5.362321, whole genome shotgun sequence.
ACCESSION   DAOOBH010000112 DAOOBH010000000
VERSION     DAOOBH010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12601_length_7104_cov_5.362321"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..313)
                     /locus_tag="PK218_09835"
                     /pseudo
     CDS             complement(38..313)
                     /locus_tag="PK218_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
     gene            complement(344..1705)
                     /locus_tag="PK218_09840"
     CDS             complement(344..1705)
                     /locus_tag="PK218_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPW98850.1"
                     /translation="MYCILDIETTGGQFNEEGITEIAIYKFDGYEVVDQFISLVNPEK
                     PIQPFVVKLTGINNAMLRSAPKFYEVAKRIIEITDGCVIVAHNSSFDYRILRTEFTRL
                     GYDFVKETLCTVELSKKLIPGQLSYSLGKLVRALGIPVTDRHRASGDAMATVKLFKML
                     LAKDSKKEIITELIKKEIKSGLTPKLLDIVESLPTKTGIYYIHNERGGLIYIGKSKNI
                     KKRVNQHFTGTSGKSKKIQREVFAVTYEVTGSELIALLKESEEIKINKPIYNRSQRKS
                     IFQWALYAEKDKKGYLNLKLLKADGRKKEITSFTSIQEGKNALFKITEKYNLCQKFTG
                     LYETQNGCFQYKIKECNGACLGNETPEDYNFRVNKFMTEMKFENDNMVIIDRGRSIEE
                     RCAVLIESGIYKGYCFYDLNYQINNVDILKNIIIPMQNNRDTRTIIQGYLKRNKVMKI
                     IKF"
     gene            complement(1739..2401)
                     /locus_tag="PK218_09845"
     CDS             complement(1739..2401)
                     /locus_tag="PK218_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPW98851.1"
                     /translation="MSIANNLQSIKSQLPTNVTLVAVSKTKPVSDLMEAYNAGQRIFG
                     ENKIQEMTEKHQQMPKDIYWHMIGHVQSNKVKYMIPYVKLIHGVDSLKLLKEINRHAL
                     RWRRKVDCLLQIHIAEEDTKFGLDENELNDILNSEEFKGFNNVKVIGLMGMATFTDNQ
                     HQIKREFEHLKSIFDKLSKQPTTQNLQLTTLSMGMSGDYQLAIECGSTMVRIGSSIFG
                     IR"
     gene            complement(2456..3682)
                     /locus_tag="PK218_09850"
     CDS             complement(2456..3682)
                     /locus_tag="PK218_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPW98852.1"
                     /translation="MSKIIPFKAVRPSPDKVALVTCRNYEDYSQAELAAWLNFNPYSF
                     LHVINPAYVNAQKITLDKRFKGVAHKYQDFKNEGIFVSEENPVFFLYEIQTKNSTYTG
                     IVAGTSIDDYKNNVIKRHEDTLQYRVELFKDYLHQTGFNTEPVLITYPDNEAINSWIA
                     NKKIERPIYNYSTTNKEKHQLWKIETDNEISWLQNQFELIPELYIADGHHRSASAELL
                     YDEDNHLGNTNLKYFMSFLIAESNVKIYEYNRIIRDLNGFSTTDFLEKLTENFIIKDK
                     EQELWKPQSKFEFGMYLDGNFYALFYKQESKSEDKNTILNNLDAQILYDKVLQPLLAI
                     DDLRNDERIDYIPGKQSVLTLKELVDEGEFAVGFMLFPSDISEIKALADNNLIMPPKS
                     TYIEPKFRSGLMVYEL"
     gene            complement(3774..4661)
                     /locus_tag="PK218_09855"
     CDS             complement(3774..4661)
                     /locus_tag="PK218_09855"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HPW98853.1"
                     /translation="MKQIAVIGAGTMGNGIAHTFAQSGFTVKLIDISEKSLEKGIATI
                     AANLDRMVTKGTITEDDKHKTISNIITYTDMKDGIVGCDLVVEAATENVGLKMNIFKQ
                     LSDVCNHNVILASNTSSISITQIAAQVVHPERVIGMHFMNPVPIMKLVEIIRGYSTSD
                     DVTKIIMDLSVKLGKTPTEVNDYPGFVANRILMPMINESIETLYNGVAGVQEIDTVMK
                     LGMAHPMGPLQLADFIGLDVCLSILNVMYDGFKNPKYAPCPLLVNMVMAGKLGVKSGE
                     GFYDYSESKKAEKVSKQFI"
     gene            complement(4723..5691)
                     /locus_tag="PK218_09860"
     CDS             complement(4723..5691)
                     /locus_tag="PK218_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW98854.1"
                     /translation="MLKVGVLGAGHLGKIHLRLLQQSTKYELVGFYDDNIENGKKVAA
                     EFGYKQFDTIARLMHAVDVIDIVTPTLSHYKCAKVAIKSGKHVFIEKPIATTVEEAEE
                     IIALAKEYKVKGQVGHVERFNPAFSAVKNQIENPMFIETHRLAEFNPRGTDVPVVLDL
                     MIHDIDAILSVVKSKVKAVHASGVSVLSQSPDIANARIEFENGCVANITSSRISMKNM
                     RKSRFFQKDAYISVDYLDKICEVVKMKDAPEIPGDFDMILQNAEGEKKQIYFNNPEVE
                     PNNAILDELETFAEAIKNNTTPIVTLEDGTNALRVAYMIIDSMNKK"
     gene            5843..6484
                     /locus_tag="PK218_09865"
     CDS             5843..6484
                     /locus_tag="PK218_09865"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPW98855.1"
                     /translation="MKDTNKHQGLRNQLTKQLEEKGITDKNVLDAIKKIPRHLFLNSS
                     FEDFAYQDKAFPIGAGQTISQPYTVAFQSQLLEVKQGQKILEIGTGSGYQTAVLCLLG
                     AKVYSVERQNELFKTTLNLLPKLGIRPKHLSFGDGYKGLPNHAPFDSIIVTAGAPTIP
                     KALMAQLKIGGKLVIPVGNTDQIMTMLIRKNATQFEKHEFGDFKFVPLLENKN"
     gene            6490..6750
                     /locus_tag="PK218_09870"
     CDS             6490..6750
                     /locus_tag="PK218_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98856.1"
                     /translation="MKTIFLIVLVSFLGYPQGYTSYFTGNPTNITTTPEFGVCMMGGA
                     TEHDNAMRWLLQKANGGDVVVLHSHQHQEQVHLLVAHWQLIK"
BASE COUNT         2230 a         1199 c         1088 g         2587 t
ORIGIN      
        1 taacgtggat atatttcgag aaacattatt gcaaaaaact aaaaatatat ttgaaaggct
       61 tgttaatagt aatgattctt aaaatatatt ccacactaaa aaaaagagta attatccatt
      121 ccattattac caattgatca tgataatgag catcaaatcc tttcacagtt tcaagcatta
      181 caaacagaac gcttagaagt attaagccta gcaatattaa atcaaacatt cttccccaaa
      241 aagtatttgt accataaatg ataatgtgcg ctttttgacg aaataaggta taagatgatt
      301 ttgctttact cattatagag ttagttaatt attctaagat atgttaaaat tttatgattt
      361 tcattacttt attccttttt aagtatcctt gaataatagt tcgcgtatcc cgattgtttt
      421 gcatcggaat gataatgttt tttagaatat caacattgtt tatttgataa tttaaatcat
      481 aaaaacaata tcctttataa atacccgatt caattaaaac agcgcaacgt tcttctatac
      541 ttctgcctct atcaataata accatattgt cattttcaaa tttcatttct gtcatgaatt
      601 tatttacacg aaagttataa tcttctggcg tttcgtttcc caaacaggca ccgttgcatt
      661 cttttatttt atattgaaaa caaccgtttt gggtttcata tagtcctgta aatttttgac
      721 ataaattgta tttctcagta attttgaata atgcattttt gccttcttga atggatgtaa
      781 aagaagtaat ttcttttttt cttccatcag cttttaataa ttttaaattc aaataaccct
      841 ttttatcttt ttcagcatat aaagcccatt gaaaaatact tttccgttgc gagcgattgt
      901 aaataggttt gttgatttta atttcttcgc tctcttttaa caacgcaatc aattcacttc
      961 cagtaacctc ataagttaca gcaaaaactt cgcgttgaat ttttttactt tttccagatg
     1021 ttcctgtaaa atgttgatta actcgttttt tgatgttttt acttttacca atatagatta
     1081 agccgcctct ttcgttatga atataatata ttccagtttt ggttggcaaa ctttcgacaa
     1141 tatctaataa tttaggagtc agtccggatt tgatttcctt tttaattagt tccgttataa
     1201 tttctttttt gctgtctttt gccaaaagca ttttaaacaa tttcacagtt gccatagcat
     1261 ctccactagc acgatgtcta tcggtaactg gaattcctaa tgcgcgaact agctttccta
     1321 agctatagga aagttgacca ggaattaatt ttttagaaag ttctaccgta caaagcgttt
     1381 ctttgacgaa atcataaccc aaacgtgtga attcggttcg caaaattcga taatcaaatg
     1441 aggaattatg tgccacaata acgcaaccat cagtaatttc gataatacgt ttggcaactt
     1501 cataaaattt tggcgcagaa cgcaacatag cattgttgat accagtcaat tttactacaa
     1561 aaggttgaat cggtttttca ggattcacca agctgatgaa ttgatcaact acttcgtaac
     1621 catcaaattt atagatagca atttccgtaa ttccttcttc gttgaattga ccgccggtgg
     1681 tttctatgtc gagtatgcaa taaatcgttt aaaagtttaa gggttaaaaa gttgttgatt
     1741 atcgtatgcc aaagatacta ctaccaattc gaaccatagt gcttccgcat tcgatagcta
     1801 gttggtaatc gccactcatt cccatggaca aggttgtaag ttgtagattt tgggttgtag
     1861 gttgctttga taatttatca aaaatagatt ttaaatgttc aaattctctt tttatttgat
     1921 gctgattatc agtgaaagta gccattccca tcaaaccaat tactttaaca ttattaaatc
     1981 ctttaaattc ttctgaattt agtatatcat ttaactcatt ctcgtctaaa ccaaattttg
     2041 tatcttcttc tgcgatgtgt atttgcaata aacaatctac tttccttcgc cacctaagag
     2101 catgacgatt tatctctttt agtaatttta aactatcaac accatgtatt aattttacat
     2161 aaggaatcat gtatttcact ttgtttgact gaacatgacc aatcatgtgc caataaatgt
     2221 cttttggcat ttgctggtgc ttttcggtca tttcttggat tttattttcg ccaaaaatgc
     2281 gttgtccagc attatatgct tccatcaaat cggaaactgg ttttgtttta gaaacagcaa
     2341 caagtgttac gttagtgggt aattgcgatt ttatagattg taaattgttt gctattgaca
     2401 ttttgtaaaa gtttcagtgt tcagtttttt ggtattcaat taaaacttca gcaatctata
     2461 gttcatacac catcaaacca cttctaaatt ttggttcgat ataagtactt tttggaggca
     2521 taattaagtt gttgtcggcc agtgctttta tttccgaaat atcacttgga aacaacataa
     2581 atcctacagc aaactcgcct tcatcaacca actcttttaa agttaaaaca gattgttttc
     2641 cgggtatata gtcaatacgc tcatcatttc ttaaatcgtc gattgctaat aatggctgca
     2701 aaactttatc gtataaaatt tgtgcgtcta aattgtttaa aattgtattt ttgtcttcgg
     2761 acttagattc ttgcttataa aatagagcat aaaaatttcc atccaaatac attccaaatt
     2821 caaatttgct ttgaggtttc cacaattctt gttccttgtc tttaataata aaattttcag
     2881 ttaatttttc aagaaaatca gttgttgaaa aaccattcaa atctctaatg attctattgt
     2941 attcatatat tttaacattg ctttcggcaa ttaaaaaact cataaagtac tttaaattag
     3001 tatttcctaa atgattgtct tcatcatata acaattcggc agaagcggat cgatggtgtc
     3061 catcggcgat atataattct ggtattaatt caaactggtt ttgtaaccaa gaaatttcat
     3121 tatcggtttc aatcttccaa agttgatgtt tctctttatt ggttgttgaa taattataaa
     3181 ttggtcgttc aatttttttg tttgcaatcc aagaattgat ggcttcatta tccggataag
     3241 ttatcagtac gggttctgtg ttaaaacctg tttggtgtaa ataatcttta aataattcta
     3301 cgcgatattg caatgtgtct tcgtgtcttt tgatgacgtt atttttataa tcatcaattg
     3361 aagttccagc cactatacca gtatatgtac tgtttttagt ttgaatctca tacagaaaaa
     3421 agacaggatt ctcttctgat acaaaaatac cttcgttttt aaaatcttgg tatttatgcg
     3481 ctactccttt aaaacgctta tctagagtga ttttttgtgc atttacataa gctggattaa
     3541 taacatgtaa aaacgaataa ggattaaagt tcaaccaagc tgctaattct gcttgcgaat
     3601 aatcttcgta atttctgcaa gtgactaatg ctactttatc tggcgatggg cgaacagctt
     3661 tgaatgggat tattttactc atgttattta accactaagg tcacaaagtt ttacacaaag
     3721 tacgcgaaga tttttgtgag ctttgcgcct tcttcgtgca ctttgatgtt attttatata
     3781 aactgcttag aaactttctc cgctttttta ctttcggaat aatcataaaa accttcaccc
     3841 gattttactc ctagttttcc tgccattacc atattgacta aaagcggaca aggtgcatat
     3901 tttgggtttt tgaaaccatc atacattaca tttaaaatag aaagacaaac atctaaacca
     3961 ataaagtctg ctaattgtaa tggtcccatt ggatgtgcca ttccgagttt catcactgta
     4021 tcaatttctt gaacgcctgc cacaccattg tataacgttt ctattgattc gttaatcatt
     4081 ggcattaaaa ttctgtttgc cacaaaacca ggataatcgt taacttctgt tggtgttttg
     4141 cctaatttta cagataaatc cataattatt ttggtaacgt catcagaagt tgaatagcca
     4201 cgaatgattt cgactaattt catgattggt actggattca taaaatgcat tcctattacg
     4261 cgttctgggt gaacaacttg cgcggcaatt tgagtaatcg aaatggaaga tgtgtttgaa
     4321 gcaagaataa cgttatggtt acaaacatcg cttaattgct taaaaatatt catttttaaa
     4381 cctacatttt cagtggcagc ttcaacaacc aaatcgcaac ctacaattcc atctttcatg
     4441 tcagtgtaag tgattatatt tgaaatggtt ttgtgcttat catcttctgt gatagttcct
     4501 tttgtgacca ttctatctag gtttgcggct attgttgcta ttcctttttc taatgatttt
     4561 tcggaaatat cgattaattt tacagtaaat cctgattgtg caaacgtgtg agcaattccg
     4621 ttgcccattg ttcctgcacc aattacagct atttgtttca ttaatattgt ttaaaaggtt
     4681 ataagtttaa aagtttatga gtttatgcgt ctaactaaca ctttacttct tgttcatcga
     4741 atcaataatc atataagcca ctcgaagtgc atttgttcca tcttctaaag taacaattgg
     4801 agtagtgttg tttttaattg cctctgcaaa agtttcaagt tcatccaaaa tggcattgtt
     4861 tggttcaact tctggattgt tgaaataaat ttgctttttt tcgccttcag cattttgtaa
     4921 aatcatatcg aaatctccgg gaatttcggg agcgtctttc atttttacaa cttcgcaaat
     4981 tttatctaaa taatcaactg atatgtaggc atctttttga aaaaaacgcg attttcgcat
     5041 atttttcatt gaaattctac tcgaagtaat attggcaaca cagccgtttt caaattcaat
     5101 ccgagcattg gcaatatctg gcgattggct tagaacggaa acgccactgg catgaacagc
     5161 tttcactttt gattttacaa cactcaaaat agcatcgata tcgtgtatca ttaaatccaa
     5221 tactacagga acatctgtgc cacgaggatt aaattctgcc aaccgatgcg tttcaataaa
     5281 catagggttc tcgatttgat ttttaaccgc cgaaaacgct ggattaaaac gctctacatg
     5341 accaacttga cctttaactt tatattcttt ggctaaggct ataatttctt ctgcttcttc
     5401 aactgttgta gcaattggtt tttcaataaa aacatgtttc cctgacttta tggcaacttt
     5461 ggcacacttg taatgcgaaa gcgtgggagt tacaatatca ataacatcaa cagcatgcat
     5521 aagtcttgcg atggtgtcaa attgtttata accaaattca gcagcaactt ttttaccgtt
     5581 ttctatgtta tcatcataaa agccaacgag ttcgtattta gtagattgtt gtaataatcg
     5641 taagtgaatt tttccgagat gaccagcacc taaaacgcca acttttagca taaaaataaa
     5701 ttttatcaaa agtactaatt ataaaataaa ttccaatatg aaaatttcaa attccaattg
     5761 tagaatagcc gaaattattt gatgtttcca atttgtttat tggatttttt aaatctattt
     5821 ttacgaaata atttttccca aattgaaaga tactaacaaa catcaaggtc tcagaaatca
     5881 attaacaaaa caattagaag aaaaaggaat tacggataaa aatgttttag acgctataaa
     5941 aaaaattcca cgtcatttgt ttttgaattc tagttttgaa gattttgcct atcaagacaa
     6001 agcatttcca attggtgctg gtcaaaccat ttcgcagcct tacactgtgg cgtttcaaag
     6061 tcaattatta gaagtgaaac aaggtcaaaa aatactagaa ataggaacag gttctggtta
     6121 tcaaacggcg gttttgtgtt tgcttggtgc caaagtatat tcggttgaaa gacaaaatga
     6181 attgttcaaa acaactttaa atttattgcc taaattaggc attcgtccaa aacatttatc
     6241 ttttggtgat ggctataaag gcttgccaaa tcatgcaccg tttgacagta tcattgtaac
     6301 tgctggtgcg ccaactattc caaaagcttt aatggcacaa ttgaaaatag gaggaaagtt
     6361 ggttattcct gttggtaata cagaccaaat tatgaccatg ttaataagaa aaaatgccac
     6421 gcaatttgaa aagcatgaat ttggtgattt taaatttgta cctttattag aaaataaaaa
     6481 ttaaagaatt tgaaaacaat tttcctaatc gttttagttt catttttagg ttatccacaa
     6541 ggttacacga gttattttac aggaaaccca actaatataa caacaactcc agaatttgga
     6601 gtttgcatga tgggtggcgc aacggaacat gataacgcta tgcggtggct gttgcaaaaa
     6661 gcaaatggtg gtgatgttgt tgtgcttcat tctcatcaac atcaggaaca ggtccatctg
     6721 ttagttgctc attggcagtt aattaaataa ttttaatgtc tttccaaacc cgttttctca
     6781 atctttaaat atagaaaacg cattaaatac aaccgtagaa atctacgata gttttgggaa
     6841 aagggttttt gttgaagaag tttataactc cagttcaatt gatacctctc atctttcaag
     6901 cggattgtac attatgaaaa ttgagaagta aggaaaaatt aacactaaaa aaatagtgaa
     6961 aaattaattc cactatttcg gtttgatgat atctaggtat cgttgaagac tttctttttc
     7021 cgtttgtgca ataaatagca aaaattcttc tttattattt ttggtttgag ctttttccaa
     7081 agtttgataa tatttcattc tagt
//
LOCUS       DAOOBH010000113         6936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_12956_length_6936_cov_7.110013, whole genome shotgun sequence.
ACCESSION   DAOOBH010000113 DAOOBH010000000
VERSION     DAOOBH010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12956_length_6936_cov_7.110013"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PK218_09875"
     CDS             complement(<1..572)
                     /locus_tag="PK218_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HPW98857.1"
                     /translation="MKKLLYIFLLFTGIANAQIVNIPDANFKAKLIQLGVDANADGEI
                     QVSEALIPTNIDVSSSNISDLTGISAFTNLTNLYCNDNTITSINASSLTHLVGLACYN
                     NQLTNLNVTGLNQLYNLYCSNNLLTSLDVSGLTNLYYLYCFQNQLTTLNVTGLPLLDT
                     IHCYSNQLTSLTLSNLPNLRFLNCEYNSLTS"
     gene            complement(663..1421)
                     /locus_tag="PK218_09880"
     CDS             complement(663..1421)
                     /locus_tag="PK218_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPW98858.1"
                     /translation="MITAILIEDDHNLRNGLKALLEKTAPDIKIIGEADSVVSGIQLL
                     SNLQPQVVFLDIHLGDGSGFDILEEINNKGKLNSQIVFITAHEQYAIKAFRFSALDFL
                     LKPVDPDELLKVIDKIKAVVGKNDTVAHIDLLLENIRKKVDNFKRIALSNADGMHLFD
                     ISDIIRCESEDNYTKFYIKNNKPILISKTLKDYEELLSEHGFERIHQSHLINLSYLKS
                     YIKKDGGYVVMADNSNLPVSQRKKERLQELLKTI"
     gene            complement(1511..3460)
                     /locus_tag="PK218_09885"
     CDS             complement(1511..3460)
                     /locus_tag="PK218_09885"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW98859.1"
                     /translation="MKIKHTLFIVLLAAMASFAQTSKLDSLMVLLPNTKNDIDKVQLY
                     NAIAEEYKTSNPNLMLQYANKAEALAKKIDYKTAEGYALLHKGNANIILGNYKEALLL
                     FSDAQHLFESLDAKSENKKGLAKALGSIGIVFSEQSNYSRALQYYLKSVKIYEALKDE
                     VSLAKLYNNIGIVYHSQGNEFKSLEYFSKAQKTQERLRDKNIGITYTNIANSYLKQNN
                     APKALEYYQLAKKALDLNQNPRALGEWYNNMGLYYQAETNNPKAIESWQNAIQTFQTI
                     DDKFGMADTYIYLAQLYLSQNELEKTKQFANQSLALATELEVLEQIMLSEKLLSDCYA
                     KQNNATLALEHFKRYSQAKDSLTNEATIRKSVEEELNFEFDKREALQQKEIEKRDLLL
                     KEESKRNTMQLIFAALFGLLLFGFIFLIYNRMQLKKNLTLQKELSEYEQKALHLQMNP
                     HFVFNCLGSISSFIVQNGTDSAIKYLSKFSKLMRLTLEYSKESLIPIDKEIEGLQNYL
                     ELEQLRFNNMFSFTITKSNAIDDDVALPPLLLQPFIENAIIHGVIPKKEMGSIAIDFI
                     IEKESLVCIIEDNGIGFEQSKIAKKDSVTAHKSMALDITKKRLELMETSTSMKANVEI
                     IELKNDKNEIQGTKVILKIPVQYIK"
     gene            complement(3802..4995)
                     /gene="sucC"
                     /locus_tag="PK218_09890"
     CDS             complement(3802..4995)
                     /gene="sucC"
                     /locus_tag="PK218_09890"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPW98860.1"
                     /translation="MNIHEYQGKEILASYGVRIQRGIVANNPVEAVAAAKQLTAETGT
                     GWHVIKAQVHAGGRGKGGGVKLAKNLEQVEQIAEQIIGMMLITPQTSAEGKRVHKILV
                     AEDVYYPGESETSEFYMSVLLNRATGRNMIMYSTEGGMDIEEVAEHTPHLIFTEEIDP
                     AVGLQGFQARRVAFNLGLSGNAFKEMVKFVDCLYKAYIGSDASMFEINPVLKTSDNKI
                     MAVDAKVNIDDNALYRQAKIAEMRDIREENPIEVEAKEVGLNYVDLDGTVGCMVNGAG
                     LAMATMDLIKYAGFEPANFLDVGGTADAKRVETAFRIILRDPNVKAILINIFGGIVRC
                     DRVAQGVVDAYKNMGDAIKVPIIVRLQGTNAEIAKELIDNSGMPILSAVQFQEAANQV
                     KAALS"
     gene            5194..6399
                     /gene="lysA"
                     /locus_tag="PK218_09895"
     CDS             5194..6399
                     /gene="lysA"
                     /locus_tag="PK218_09895"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPW98861.1"
                     /translation="MKTTDLLQLAEEFGNPLYVYNAEKIEQQYKRLTGAFAKVEKLRI
                     NYAMKALSNVSVLKLLKNLGSGLDTVSIQEVQLGLHAGFTPDKIIFTPNGVSFEEIEE
                     VAKLGVQINIDNLSVLEHFGTNHPKTPVCIRINPHVMAGGNSNISVGHIDSKFGISVH
                     QLPHLLRIVENTKMNINGIHMHTGSDILDIDVFIYAAEILFNTAKHFKDLEFLDFGSG
                     FKVPYHKGDIETNIEELGKKLTKRFLAFEKEYGRELTLAFEPGKYLVSEAGYFLAKVN
                     VVKQTTSTVFAGIDSGFNHLIRPMFYGSQHGIDNISNPKGKERFYTVVGYICETDTFA
                     NNRRIAEIHEGDVLCFRNAGAYCFSMASNYNSRFKPAEVLWKNGKGHLIRERETMDDI
                     LKNQIIVEV"
BASE COUNT         2081 a         1225 c         1160 g         2470 t
ORIGIN      
        1 gatgttaatg agttatattc acaatttaaa aaccttaaat ttggtaaatt agataaagtt
       61 aaagatgtaa gttgatttga ataacaatgg attgtgtcta gtaaaggtaa cccagttaca
      121 tttaaagtag ttaattggtt ttgaaaacaa tataagtaat agagattcgt taatcccgaa
      181 acgtctaagg atgttaagag gttatttgaa caatagagat tataaagttg gtttaatcca
      241 gttacattta aattagttaa ttgattatta taacaagcta aaccaactaa gtgagtcaat
      301 gaactagcgt ttatactggt tattgtatta tcattacaat acaaattggt caaattagta
      361 aatgcagaaa tacctgttaa atcggatatg ttagatgaac tgacatcaat atttgttgga
      421 attaatgctt ctgagacctg aatttctcca tcagcattag catcaactcc tagctgtatt
      481 agtttcgcct taaaattcgc atcaggaata ttgacaatct gtgcgtttgc aattcctgta
      541 aataatagaa atatgtataa tagttttttc ataacgtttg tttttgactt ctataaaatt
      601 atgtaatctt ttggttttta tttttgataa atgagtattc tagtgtttca aattagaatt
      661 cgttagatgg tttttaacaa ttcctgcaag cgttcttttt ttcgttgtga aaccggaagg
      721 ttactgttgt ctgccatgac cacataaccg ccgtcttttt tgatgtacga tttcaaatag
      781 gacaaattga ttaaatgact ttgatgaatg cgttcaaatc catgttcgga taagagttct
      841 tcataatctt tcaaggtttt tgaaatcaaa attggtttgt tatttttgat ataaaacttg
      901 gtataattgt cttcactttc gcaacgaatg atgtcgctaa tgtcaaaaag atgcatccca
      961 tcagcattag acaaagcaat tcgtttaaaa ttatctactt ttttccgaat gttttctaac
     1021 aataaatcaa tgtgtgctac cgtatcattt ttgccaacaa cggcttttat tttatcaatc
     1081 acttttagca attcatctgg atcaactggt ttcaatagaa aatccaacgc actaaatcgg
     1141 aatgctttga tagcatattg ctcatgcgcc gtgatgaata ctatttgcga attcaacttt
     1201 cctttgttgt taatttcttc caaaatatca aaaccagaac catcaccaag atgaatatcc
     1261 agaaaaacaa cttgcggttg caaattcgat aaaagttgaa ttcccgaaac aacgctgtct
     1321 gcttcaccaa ttattttgat atcgggtgct gttttttcaa gtaatgcttt tagtccgttt
     1381 cttaaattgt ggtcgtcttc tatgagtatt gctgtaatca ttttttttta ggtttaagtg
     1441 tttaaaaggg tataagttta tgagttaaga caatactttt aaccacataa ccttataaac
     1501 ttttaaccct ttactttata tattgcaccg gtatttttaa aattactttt gttccttgaa
     1561 tttcgttttt atcatttttc aattcaatta tttccacatt tgctttcatc gaagtagagg
     1621 tttccattaa ttctaatcgc tttttggtaa tgtccaatgc catcgatttg tgtgctgtta
     1681 cggaatcttt tttggcaatt ttcgattgtt caaaaccgat tccgttgtct tcaataatgc
     1741 agaccaaact ttctttttca ataataaaat ctatagcaat acttcccatt tctttctttg
     1801 gaatgacacc atgaatgatg gcgttttcta taaaaggttg caataacaat ggtggcaaag
     1861 ccacatcgtc atcaatagca tttgatttgg tgatggtaaa cgaaaacata ttgttgaaac
     1921 gcaattgctc caattccaaa tagttttgca aaccttcaat ttctttatca attggaatta
     1981 aactttcctt agaatattct aaagtcaatc gcattaactt agaaaacttt gataaatatt
     2041 ttatcgccga atcagtaccg ttttgcacaa taaaactcga aatcgaaccc aaacaattga
     2101 atacaaaatg tggattcatc tgcaaatgca acgctttttg ttcgtattct gataattctt
     2161 tttgcaaggt taagtttttc ttgagttgca ttctgttata gattaagaaa ataaacccaa
     2221 ataataacaa tccgaatagt gctgcaaaaa taagttgcat ggtgttgcgt ttcgattctt
     2281 ctttgagcaa taaatcgcgc ttttcgattt ctttttgttg taaagcttcg cgtttatcaa
     2341 actcaaaatt caattcttct tctacgcttt ttctgatggt tgcttcgttg gtcaaactat
     2401 ctttggcttg gctgtaacgt ttaaaatgtt ctagtgctaa agtggcgtta ttttgtttgg
     2461 cataacaatc gcttagtagt ttttcggaaa gcatgatttg ctctaaaact tccaattctg
     2521 tagctaatgc taacgattga ttggcgaatt gtttggtttt ctccaattca ttttgtgaca
     2581 aatacaattg tgccaaatag atatacgtat ctgccattcc gaatttatcg tcgatagttt
     2641 gaaaggtttg aatagcgttt tgccaacttt caatggcttt aggattgttt gtttcagctt
     2701 gataatacaa acccatattg ttgtaccatt cgcccaaagc tctaggattt tgatttaagt
     2761 ctaacgcttt ttttgccaat tgataatact ccaaagcttt tggcgcattg ttttgcttta
     2821 aataactgtt ggcaatgttg gtataggtta tcccaatatt tttatctcga agtcgttctt
     2881 gagttttttg tgctttacta aaatattcta aactcttgaa ctcattgcct tgcgaatgat
     2941 agacaatccc gatgttgttg tataatttag ctaaagaaac ctcatctttt aaagcttcat
     3001 aaattttaac tgattttaga taatactgca atgctctact ataattgctt tgctcagaaa
     3061 aaacgatacc aatacttcct aacgcttttg ccaatccttt tttgttttca gattttgcat
     3121 caagcgattc gaaaaggtgt tgcgcatctg agaacagtaa taacgcttct ttgtaattcc
     3181 ctagaatgat gttggcgttt cctttgtgca ataatgcata accttctgct gttttgtagt
     3241 caattttttt ggctaatgct tctgctttat tggcatattg aagcatcaaa tttgggttgg
     3301 acgttttgta ttcctcggca atggcattgt acaattgcac tttgtcaata tcattttttg
     3361 tatttggcaa aagcaccatc aaactgtcta atttagaagt ttgtgcaaaa cttgccatcg
     3421 ctgccaaaag aactataaaa agtgtatgtt ttattttcac gagaaacaac tttatatgtt
     3481 tctaacaaat gtaacattta cttcaattga aaaatgggca aattagaatt tgtgggtttt
     3541 gaattagaat ttaaaacaat tgatgtgatt tatttgccac agattacacg gatttttgtt
     3601 aagtattttt tttaacacct aacgaaatag atttttatta ctaaataagg agttttactt
     3661 ttttacagaa aaaacgtaga gacgtaaaaa tgcaaaaata taatccgtgt taatcagtaa
     3721 aatccgtgtc atccgtgtcc cataaaacaa aaaatcccga gccgaaactc aggattttga
     3781 tataaataat actttttctt tttatgataa agccgctttc acttggtttg ctgcttcttg
     3841 gaattgtact gccgataaaa ttggcattcc agaattgtcg attaattctt ttgcaatttc
     3901 agcgttggtt ccttgtaatc tcacaatgat tggcacttta atagcatcgc ccatgttttt
     3961 ataagcatca acaacacctt gtgctactct atcgcaacga acaattccgc caaagatatt
     4021 gataaggatt gcttttacgt ttggatctct aagaatgatt ctgaaagctg tttctacacg
     4081 ttttgcatcg gctgttccac caacgtctaa gaaatttgct ggttcaaaac cggcatattt
     4141 aatcaaatcc atcgttgcca ttgccaaacc tgcaccattt accatacaac ctactgttcc
     4201 gtcaaggtca acatagttta atcctacttc ttttgcttct acttcgattg ggttttcctc
     4261 acgaatgtca cgcatttctg caatttttgc ctgacggtac aacgcattgt cgtcaatatt
     4321 tactttagca tcaactgcca ttattttatt gtctgaagtt ttcaatacag gattgatttc
     4381 aaacatagac gcatcagaac cgatgtaggc tttgtacaaa caatctacaa atttcaccat
     4441 ttctttgaac gcatttcccg aaagccctaa attgaaagct actcttcttg cttgaaaacc
     4501 ttgcaatcca actgctggat caatttcttc agtgaaaatc aaatgtggtg tatgctcggc
     4561 tacttcttca atatccattc caccttctgt agaatacata atcatgtttc ttcctgtagc
     4621 tctgtttaaa agcaccgaca tgtagaattc tgaagtttca ctttcgccag gataataaac
     4681 atcttcggct actaatatct tgtgaacacg ctttccttct gcagaagttt gaggtgtaat
     4741 taacatcatt ccgatgattt gttctgcaat ttgttctact tgttccaagt ttttagccaa
     4801 cttcacgccg ccaccttttc cacgtccacc tgcgtgaact tgagctttaa taacgtgcca
     4861 acctgttccg gtttcagcgg ttaattgttt tgcggctgca actgcttcaa ctggattatt
     4921 ggctacaatt cctctttgaa ttctaacgcc atagcttgct aaaatttctt ttccttggta
     4981 ttcgtgaatg ttcatattga ctatttagtg tgtgtatatt atcgtttttg cactcatctt
     5041 tactaaatgc gtagcaaaag taattaatta gaatgaaacc aaaaaaagta aattctatta
     5101 gttgattaaa atttactgtt taattactaa attaatccca aataggtttg aattgcaact
     5161 tttggagtct atttttgcga aaaatttttt aaaatgaaaa caacagactt acttcaatta
     5221 gccgaagaat ttggaaatcc actttatgta tataatgctg aaaaaattga gcaacaatac
     5281 aaacgattga caggtgcttt tgctaaggtg gaaaagttgc gcatcaatta cgccatgaaa
     5341 gccttatcaa atgtttcggt acttaaacta ctgaaaaatt taggttcagg attggacacg
     5401 gtttctattc aagaagtaca actaggttta catgctgggt ttacgcccga taaaattatt
     5461 tttacgccaa acggtgtttc gtttgaagaa attgaagaag tagccaaact gggtgttcaa
     5521 atcaatattg acaacctttc ggtcttggaa cattttggca ccaatcatcc aaaaacgcct
     5581 gtttgtattc gtatcaatcc acacgttatg gcgggcggaa acagtaatat ttcggtaggt
     5641 catattgata gtaagtttgg tatttcggta catcagttgc cgcatttgtt gcgtattgtt
     5701 gaaaacacca aaatgaacat caacggtatt cacatgcata cgggttcgga tattctagac
     5761 attgatgtct tcatctatgc tgccgaaatt ttattcaaca ctgccaaaca tttcaaagat
     5821 ttagaattcc ttgattttgg ttctggattt aaagtgcctt atcacaaagg cgatatcgaa
     5881 accaacatag aagaattggg caaaaaacta accaagcgtt tcctagcgtt tgaaaaagaa
     5941 tacggtcgcg aattgacctt agctttcgaa ccgggtaaat acctggtaag tgaagcgggt
     6001 tatttccttg caaaagtaaa tgtggtaaaa caaactacct caacggtttt tgctggaatt
     6061 gatagtggtt tcaaccattt aatccgacca atgttttatg gttcgcaaca tggtatagac
     6121 aatatttcaa acccaaaagg caaagaacgt ttttataccg ttgttggtta catttgcgaa
     6181 accgacacgt ttgccaacaa ccgcagaatt gccgaaatcc acgaaggcga tgtattgtgt
     6241 ttccgcaatg ctggtgccta ttgcttctcg atggcatcaa actataactc acgtttcaaa
     6301 cctgccgaag tattgtggaa aaacggaaaa ggtcacttaa ttcgtgaacg agaaacaatg
     6361 gatgacatct tgaaaaatca aattatagtt gaagtataga ccacttagac tgtttagata
     6421 atttaaactg cttagacttt agtttaagca gttttttttt ctagcttctc gatactattt
     6481 ttaaaattac aaactagcgt ttgcattttt aaaaatcact cgaagtgacg tagagtgcgc
     6541 aacgtttttc ctcgccgtct gttcgagtgg tttttgtgga acgtagtgga gcaaaaagtg
     6601 tatcgagaac ttatctctta ccaacgcttc tcgatactat tttcaaaact acaaactagc
     6661 gtttgtattt ttaaaaatca ctcgaagtga cgtagtttgc gcaacgtttt ttcctcgccg
     6721 tctgttcgag tggtttttgt ggagcgtagt ggagcaaaaa gtgtatcgag aacttctttc
     6781 ttaacaacgc ttctcgatac tattttcaaa actacaaact agcgtttgca cttttaaaaa
     6841 tcactcgaag tgacgtagtt tgtgcaacgt tttttcctcg tcgtctgttc gagtggtttt
     6901 tgtgtaatga aatggagcaa aaattgtatc gagaac
//
LOCUS       DAOOBH010000114         6742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_13412_length_6742_cov_4.855541, whole genome shotgun sequence.
ACCESSION   DAOOBH010000114 DAOOBH010000000
VERSION     DAOOBH010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13412_length_6742_cov_4.855541"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PK218_09900"
     CDS             complement(<1..404)
                     /locus_tag="PK218_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HPW98862.1"
                     /translation="MSSKETYKHLNEYLDNHYIHKSNWLRAAVLGANDGILSTASIAI
                     GVAAASSTREPIILATLAGLVAGALSMAAGEYVSVSSQTDLETADINREREELLEMPE
                     LELKRLAQIYEQRGLKKETALQVAIEMTEHDA"
     gene            complement(401..835)
                     /locus_tag="PK218_09905"
     CDS             complement(401..835)
                     /locus_tag="PK218_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98863.1"
                     /translation="MNPILRNILGVLAGVLAGGMLNMGIILISGSIIPPPEGTDMTTT
                     EGLKAAMHLMEAKHFIMPFLAHALGSLLGAFVAAKLVATHKMKFAIGVGIWFFLGGVS
                     MVFMMPSPLWFTVLDLGVAYIPMAYFGGMLATNNQVKTLQLL"
     gene            complement(855..1298)
                     /locus_tag="PK218_09910"
     CDS             complement(855..1298)
                     /locus_tag="PK218_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPW98864.1"
                     /translation="MTTINTYLTFDGTCEAAFNFYKSVFGGEFDYIGKFKDMPADPNC
                     KVDEADHEKIMHVSLPISKETRLMGSDTGGEWASKLVTGNNFSISINTESKQDADSLF
                     NKLSKNGTITMPMEETFWGAYFGMFTDQFGIQWMVNYDDPAKGNF"
     gene            complement(1738..2088)
                     /locus_tag="PK218_09915"
     CDS             complement(1738..2088)
                     /locus_tag="PK218_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPW98865.1"
                     /translation="MKYLLFFLCVFFNLQTSNAQKKSTNQKAVIKTTIYCDHCNQCET
                     CGQNFQSNVYKIKGVKMYELNAEDMTITVYYNTKKTDLETIKTAISKLGYDADEVKAD
                     AVAYENLDNCCKKA"
     gene            2275..2748
                     /locus_tag="PK218_09920"
     CDS             2275..2748
                     /locus_tag="PK218_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98866.1"
                     /translation="MKTLLKSVYTFALLLCFLVSGNGFAQRIEHNVPIIVMDAFQKKF
                     PNKEPVWFREYQGRFDQKLVYEARFMFDNRYSKAFYENEGKMLAFATVVEKSELPEKV
                     LTYMKEKYPAFPIAESLLVTYSTNEVQFEVGIYIDNNFLVQVFSKDGDFIKMTKG"
     gene            complement(2845..3087)
                     /locus_tag="PK218_09925"
     CDS             complement(2845..3087)
                     /locus_tag="PK218_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98867.1"
                     /translation="MSNIIYLLFLKNNNTFLYFIKTIREVENYSDATGKVELVSGYNH
                     AWSRSDGSSFIMSDNPNFDPSSVFQDQRWKEMKKVE"
     gene            complement(3080..4900)
                     /locus_tag="PK218_09930"
     CDS             complement(3080..4900)
                     /locus_tag="PK218_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPW98868.1"
                     /translation="MPTKKASLAKIIVRNILRRVEDSITLLKVRFTERQFIYLASVLI
                     AISCALAVIVLKYFAHKVFLFANYVNTYLKLPYINSILPIVGILLTVFVVKHFLGGHI
                     EKGSSKILYAIAKKGGFIGKKQMYAQIVTSSLTVGLGGSAGLESPIVITGAAFGSNFA
                     QKYHLSQKERVLLLACGVAAGISAAFNAPIAGVLFAIEVVLVDVAISAFIPIMISAAT
                     GALISTIVLKEDVLLSFRQEEQYDYHNIGYYILLGILAGFASVMYSRHFRAIENYFHH
                     RKMKVYNKALLGASLLALLIFFSPTLFGEGYESIKTLTSSNPNVLLENTLFDEHKSNE
                     WLLLLFVGATIVLKAFASGLTIGAGGNGGNFAPSLFVGSYLGFFVSKFFILTGINTNL
                     PVANFTVVGMAGILSGLFHAPLTAIFLIGEITGGYDLMVPLMIVSSISYAVSKRFESH
                     SIDVKGLAAKDEAYTSDKDRNVLLNIDVNKIIATDYATLTLDQSLQKVVEIFSTSNQK
                     IIPILDENKILLGVVEYENVKAFIFNPYRIKFTPIEEIMTPPKAKINVEDGMIKIIKS
                     FDKSKAKELPVLKHGVYHGCLSKLRILEKYRVKLKEMVIE"
     gene            complement(4931..5608)
                     /locus_tag="PK218_09935"
     CDS             complement(4931..5608)
                     /locus_tag="PK218_09935"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPW98869.1"
                     /translation="MDFITELDIAFQKIASKESATPMENYMKNKFSFLGIKTEPRRTV
                     FKKLYEKHKSEIKANFRNMIWELLNKTEREFHYVALDLSIKEFKKNYLQEDINLIEKL
                     ITTNSWWDTVDAFAKYLVGGYLHQFPKETYVVIEHFSNSKNMWLNRTAILFQLSYKEK
                     TNFDLLKAECEKHKHSNEFFIQKAIGWALRDYSRFNPDGVSNYVTSTNLKPLSKREAI
                     RNLHKNL"
     gene            complement(5619..>6742)
                     /gene="ggt"
                     /locus_tag="PK218_09940"
     CDS             complement(5619..>6742)
                     /gene="ggt"
                     /locus_tag="PK218_09940"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008638463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPW98870.1"
                     /translation="TLYNQKFDVGDTIKYLALANTLKRICRKGEKEFYKGRTAKKLAA
                     FIQSKGGILSMEDLKKYKTVWRKPINFQYKDLNIISMAPPSSGGITLGQIMKMIEPFP
                     LNSYLYNDTKYIQLISEAERRAYADRNYFLGDPDFVKIPEKELLDTQYLNKRMQSFSF
                     DNPTPSSEVSHGETQIVESDETTHFSIIDAFGNAVSVTTTLNGAFGSKLYCDELGFFL
                     NNEMDDFASKPGVPNSYGLLGAEANSIAPQKRMLSSMTPTIVEQNGKLLMILGSPGGS
                     TIITSVLQTILNVYEFKMGMQDAVNQPRFHHQYLPDAIMLEPNNFSNAVKEELIEIGY
                     DINERFAPVLGKIDAILVLPNGTLEAGADGRGDDKAAGF"
BASE COUNT         2061 a         1201 c         1212 g         2268 t
ORIGIN      
        1 aaagcgtcgt gttccgtcat ttctatggct acttgcagtg cggtttcctt tttaagacct
       61 ctttgttcat agatttgtgc taggcgtttc agctctaatt cgggcatttc taggagctct
      121 tctctttccc gattgatgtc ggcagtctcc aaatcggttt gagaactcac tgaaacatac
      181 tcgcccgccg ccatagataa agcaccggca accaagccag caagtgtagc aagaataata
      241 ggttctcgag tactacttgc agcagcaacc ccaattgcaa tactggcagt agatagtatg
      301 ccgtcgtttg cgcctaaaac tgcagcacga agccaattgc ttttatggat gtaatgattg
      361 tctaaatact cgtttaaatg tttgtaagtt tcctttgaac tcataataat tgtaacgttt
      421 taacctgatt gtttgtggct aacattccac caaaatacgc cattggaata tatgctaccc
      481 ctaaatcgag cactgtaaac cacaatggag atggcatcat aaagaccatg ctcactcctc
      541 cgaggaaaaa ccaaatgcca acgccaattg caaacttcat tttgtgcgta gctactagtt
      601 tggcagctac aaaagcacct aaaagactcc cgagagcatg cgccaaaaac ggcatgataa
      661 agtgctttgc ttccattaaa tgcatggctg cttttaagcc ttcggtggtg gtcatgtctg
      721 tgccttcggg tggtggaatg atggagccac tgatgagtat aatacccata ttgagcatac
      781 cgccagctaa aacgccagcc aaaacgccaa ggatgtttcg taaaataggg ttcatatatt
      841 ttggttttta ttggttagaa atttcctttt gctgggtcgt cgtagttaac catccattgg
      901 atgccaaact ggtcggtaaa cattccaaaa taggctcccc aaaaggtttc ctccattggc
      961 atggttatcg ttccgttttt ggaaagtttg ttgaaaagcg aatcggcatc ttgcttactt
     1021 tcggtattga tggaaatcga aaaattgttt cccgtcacca gtttagaagc ccattctccg
     1081 ccagtgtcgc ttcccataag tcgggtttct ttgctgatgg gtaacgaaac gtgcattatt
     1141 ttttcatggt cggcttcgtc aactttacag ttcgggtcgg ctggcatgtc tttaaatttt
     1201 ccgatgtagt caaactcgcc accaaagacg gatttgtaga aattaaaagc ggcttcgcaa
     1261 gtgccatcaa aggtaagata ggtgtttatt gtggtcatga tgtgttggtt tttggtttta
     1321 tagtctttca aagatacaca tttgagggta caacagcatc aagaacaccg attattttta
     1381 atacttttca ggataaatct atttcatttc aatttttaaa attgacttaa aaatggattt
     1441 tacaaaaact gctaaacaac catttttgcc ctaaaaagtc catttgaaaa ctgtacgttt
     1501 ttgtatgata ggaaaaaaaa cattgatttt tctaaaccat gccctaaaac ctttctaaaa
     1561 attccaacca aaagtttttt tataggaatt aaagagaaaa atttacccga attagtattt
     1621 gaattttgat gtcgtcttat tgcgaaagaa ggaatactct cgaaatgatt tttagttgct
     1681 attttttttt gtgaaatgga cgtggtagtg tttccttcct tattttaaat aaatgagtta
     1741 agctttttta cagcaattgt ctaggttttc gtaggcaaca gcatcggctt ttacctcatc
     1801 ggcatcatag ccaagttttg aaatggcagt tttgatggtt tccaaatcgg tctttttggt
     1861 gttgtaataa acggttatag tcatgtcctc ggcattgagt tcatacatct taactccttt
     1921 tattttatag acatttgatt gaaagttttg accacaagtt tcgcattggt tacaatggtc
     1981 acagtagata gtagtcttga ttacggcttt ttgattggtg ctcttttttt gtgcgtttga
     2041 cgtttgtaaa ttaaaaaata cgcacaagaa gaataaaaga tatttcataa aataagtatt
     2101 gtttttacaa atatagatat ttttaactaa attattttca gcaaaaagtt aattttaaca
     2161 gcactttatt tttcgaaaat aatagtggtt attctagtgg aaagaggcta aaagtatgtt
     2221 aaactgcttt ctattttcat tagaaattct taattttagg gaaattaaaa aattatgaaa
     2281 actctactta aatctgttta tactttcgca ctattgcttt gttttttagt gtctggcaat
     2341 ggttttgcac agcgaataga gcacaatgtt ccgataattg ttatggatgc ttttcagaaa
     2401 aagtttccta ataaagaacc tgtttggttt agagaatacc aaggtcgttt tgaccaaaag
     2461 ttagtttatg aagcacgatt tatgtttgat aatcggtact caaaagcatt ttatgaaaac
     2521 gaaggaaaga tgttggcgtt tgcaaccgtt gttgaaaaat cagaactgcc cgaaaaagta
     2581 cttacctata tgaaagagaa gtatcctgca tttccgattg ccgaatcatt attagttact
     2641 tattctacta atgaagtgca gtttgaagtt gggatttata tagacaataa ttttttggta
     2701 caagtgtttt ctaaggatgg tgactttata aaaatgacaa aaggctaaga ttattgacgt
     2761 attgtttttg cgattcccaa tagcaaaaac taagaaaagg aggttagaat catctaatct
     2821 cctttttgtt tgttgtaaaa tgggttactc tactttcttc atttccttcc atcgttggtc
     2881 ttgaaatacc gaagaaggat cgaagttagg gttatcgctc ataataaaac tgctaccgtc
     2941 actgcggctc cacgcatgat tgtagccaga aactaactca actttgcccg tagcatcgct
     3001 ataattttcg acttcgcgaa tggttttaat gaagtacaga aaagtgttat tgttttttaa
     3061 aaataacaag tatataatat tactcaatta ccatttcttt taatttgaca cgatattttt
     3121 ctaaaatcct tagtttagaa aggcaaccat gataaacacc atgttttaat acgggcagtt
     3181 cttttgcttt agatttgtcg aacgatttga ttatttttat cataccgtct tcaacattta
     3241 tttttgcttt tggaggtgtc atgatttctt caattggagt gaattttatt cgataaggat
     3301 tgaagataaa agcttttaca ttttcatact ctacaacgcc cagtaggatt ttattttcat
     3361 caagaatggg gattattttt tgattggaag tagagaatat ttcgacaact ttttgtaaag
     3421 attggtctaa agttaacgta gcataatcgg ttgcaataat tttgtttaca tcaatgttga
     3481 gcagtacatt tcggtctttg tcactggtat aagcttcgtc ttttgcagcc aatcctttta
     3541 catcaataga gtgactttca aagcgtttgg aaacagcgta actaatcgaa gaaacaatca
     3601 tcaaaggcac cattaaatca tagccgcctg taatttcgcc aataaggaat atggctgtta
     3661 atggtgcgtg aaataatccg ctcaagatac ctgccattcc tactactgta aaattagcca
     3721 caggcaagtt ggtattaatg ccggttaata tgaaaaattt ggacacaaaa aagcctagat
     3781 aacttccaac gaaaagagat ggtgcaaagt ttcctccatt tcctccagca ccaatggtta
     3841 aaccagaggc aaaggctttg agaacaatag tggctccaac aaataataat agtaaccatt
     3901 cgttgctttt atgttcgtca aataaagtgt tttctaataa aacatttgga tttgaagatg
     3961 tcaaagtttt gatgctttca tatccctctc caaaaagagt gggtgaaaag aaaattagaa
     4021 gtgctaaaag tgaagctcct aaaagtgctt tattatacac tttcatcttc ctgtgatgga
     4081 aataattttc aatagctcta aaatgacgag aatacatcac agatgcaaat cctgctaaga
     4141 ttcctaataa gatataataa ccaatattgt gataatcata ttgttcttct tggcggaaag
     4201 ataataatac atcttctttt aaaacaatag tagaaattaa agctcccgtg gccgccgaaa
     4261 tcattatcgg aataaaagca gaaatagcaa catcgaccaa cactacttcg atggcaaata
     4321 atacacccgc aattggggca ttaaatgcag cactaatacc agctgcaaca ccgcatgcta
     4381 ggagcaatac tcgctctttt tgactaagat ggtatttctg agcaaagtta gaaccaaatg
     4441 cagctccagt gattacaatt gggctttcca gacctgccga accaccaagt ccaacggtta
     4501 acgaactagt aacaatttgc gcatacattt gttttttacc aataaaacca ccttttttgg
     4561 caatggcata caaaattttg gaacttcctt tttcgatatg accaccaaga aaatgtttga
     4621 caacaaatac ggtcaacaat atcccaacaa tgggcaaaat actgtttata taaggtaact
     4681 ttaaataggt gttaacgtag ttggcaaata aaaagacttt gtgtgcaaag tacttcaaga
     4741 caatcaccgc caaagcacaa gaaattgcta tcaatactga agccaaataa ataaattgac
     4801 gttctgtaaa acgaactttt aacaacgtaa tactgtcttc cactcttcga agtatatttc
     4861 gaacgatgat tttggctaac gatgcttttt tggttggcat tttaattatt ttacgtgcaa
     4921 aaataaggat ttataaattc ttatgtaggt ttctaatagc ttctcttttg cttaaaggtt
     4981 ttaaattggt gctagtcaca tagtttgaaa ctccatctgg attaaaccgg ctgtaatcgc
     5041 gtagcgccca accgatagct ttctgaataa aaaattcgtt ggaatgtttg tgtttttcgc
     5101 attccgcttt cagcaaatca aaattggttt tttctttata actcaattga aatagaattg
     5161 ctgttcggtt caaccacatg ttctttgaat tagaaaaatg ttcaatcaca acataggttt
     5221 ctttgggaaa ttgatgtaaa tatccaccca ctaaatattt cgcaaaagca tctacagtat
     5281 cccaccatga gttggttgta atcaactttt cgattaaatt aatatcttct tgtaggtagt
     5341 ttttcttaaa ttctttaata gataagtcta aggcaacata atgaaactca cgttcggttt
     5401 tatttagtaa ttcccaaatc atgtttctga aattagcttt gatttcggat ttgtgttttt
     5461 catataattt tttaaaaact gttcttcgtg gttctgtttt tatgcctaga aaagaaaact
     5521 tattcttcat ataattctcc atcggagtag cgctttcctt tgaagctatt ttttgaaaag
     5581 caatgtctag ttctgtgatg aaatccataa tacctatttt aaaatcctgc tgctttatca
     5641 tcgccacgac catccgcacc agcttcaagt gtgccattgg gtaatactaa tatagcgtct
     5701 atctttccaa gtacaggtgc gaatctttca ttaatatcat acccaatttc tatcaattct
     5761 tcttttacag cattggaaaa attattcggt tctagcatga tggcatcagg aagatattga
     5821 tgatgaaaac gcggttgatt gacggcatct tgcattccca ttttgaattc atatacattc
     5881 aatatcgtct gtagcaccga agtaattatg gttgaaccac ctggtgaacc cagtatcatc
     5941 aatagttttc cgttttgttc tacaatggtt ggtgtcatag aactgagcat tcttttttgt
     6001 ggcgcaatac tattggcttc tgcacctagt aatccatagg aattgggaac accaggtttt
     6061 gatgcaaaat catccatttc attattgaga aaaaaaccca attcgtcgca atacaattta
     6121 gaaccaaaag caccatttag agttgtagta accgaaacgg catttccaaa agcatcgatt
     6181 atcgaaaaat gcgtggtttc atcgctttct acaatttggg tttcgccatg actaacttcg
     6241 cttgacggtg ttggattatc aaaagagaag gattgcatgc gtttgtttag gtattgggta
     6301 tcgagtaact ctttttcggg tatttttaca aagtcgggat cgcccaagaa ataatttcta
     6361 tcggcatagg cacgacgttc ggcttcagag atgagttgga tgtatttggt atcattatac
     6421 aaataggaat ttagaggaaa aggttcaatc attttcataa tttgtcccaa agtaataccg
     6481 ccgctactcg gtggtgccat cgagatgatg tttaaatctt tatattgaaa gttaattggt
     6541 ttacgccaaa cggttttata ctttttcaaa tcttccatcg atagaatgcc acctttactt
     6601 tgaataaaag cggctagttt ctttgccgtt cgtcctttgt aaaattcttt ttcacctttt
     6661 cggcaaattc gttttaaggt attggctagt gcgagatatt taatcgtatc tccaacatca
     6721 aacttctgat tgtatagtgt tt
//
LOCUS       DAOOBH010000115         6513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_13950_length_6513_cov_7.492103, whole genome shotgun sequence.
ACCESSION   DAOOBH010000115 DAOOBH010000000
VERSION     DAOOBH010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13950_length_6513_cov_7.492103"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..993)
                     /locus_tag="PK218_09945"
     CDS             complement(130..993)
                     /locus_tag="PK218_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadS family aminoglycoside
                     6-adenylyltransferase"
                     /protein_id="HPW98871.1"
                     /translation="MNVRDEKLKSIIEWAKKNEDVRVLLLTSSLVNPLAPVDEFSDLD
                     IEFIFENNANYISDKSWTLNFGNPIAMIEEDENAFDNKHAMKMVLYEDGVKADFKLYS
                     KLNFIQETEQKELPEDWDIGYKILIDKDGITTQMLKPTYQVSIIKKPTEKEFNNLLND
                     FWWDTTYVAKCLARDEIFYAKYMSETVIRTGYLVPLIEWFIASEHNWNVTTNKYGRLF
                     KKYLNQEMWAKTENTFSGSNIEDNWTALFSMVDLVSEIGTELSEKLDYKYPDKLEKDI
                     RKYLTELKLKT"
     gene            complement(1264..1623)
                     /locus_tag="PK218_09950"
     CDS             complement(1264..1623)
                     /locus_tag="PK218_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98872.1"
                     /translation="MISKEEARKKLLIGYVFAGLGLIIGSSYDVYDNPFLSLILIGYF
                     VWSGYWGFSIVYKPINSFFSNMIVIEDNLYKLFIEFIMKKLIVLSIILAIGLIVGSIG
                     GAVYMQITLSSIAYGKN"
     gene            complement(1682..1861)
                     /locus_tag="PK218_09955"
     CDS             complement(1682..1861)
                     /locus_tag="PK218_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98873.1"
                     /translation="MAAERIGSVKSGKSRKSFDVKWDQASRDVYVSWAGWTSVGKASS
                     AREAMNKAEAYLYDK"
     gene            complement(1877..2959)
                     /locus_tag="PK218_09960"
     CDS             complement(1877..2959)
                     /locus_tag="PK218_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW98874.1"
                     /translation="MIDYYKILDVPMTAQSEEIKVAIKDKRRLWLQKTNAPQLERRQE
                     AEVKVKQIDEAEKILLDSSKRSEYDKKLKTSSQTDERTVDEKELKVADELVKQGWEAL
                     ISDNIADAIYIGTKATEKQGDNPEAWALLAQAKYRWGEIEDSIYEYKKAIKLRPNNSE
                     YYFDLGSVYESQQRWQDAVDNYQKASKIDPKVTVYRAAIGQVYLRVEMYEDAIQILES
                     CVSEEPDNTTYTWFLALAYNDLVLKKYMVESNDGSYYLVSKEASEFALKYFTKALALR
                     FEDDELKAIIRKNLDVANWSLSKHWGRSIGSTIKWVLILGIGGIGLFASGEAALILIA
                     LAVWALWIFFGFKEGYKINKKVLSNN"
     gene            complement(2998..4518)
                     /locus_tag="PK218_09965"
     CDS             complement(2998..4518)
                     /locus_tag="PK218_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPW98875.1"
                     /translation="MSKVIGIDLGTTFSAVAFINESGKPEIIPNRDGERIMPSVVFFE
                     DGSPIVGVTAKSSSIMEPLNVVQFVKRQMGNPSFKFITEKGDEYTSEEISAIILKRLK
                     EDAEEYLNDTIAGAVITVPAYFNDAQRKSTQDAGNIAGLNVLRIINEPTAAALAYSYD
                     RFNEPQTLLVYDLGGGTFDVTIMNIKSGEVNVLSTGGDKNLGGFDWDNEIMNYLNEEF
                     KNKTKTDLFDTPESEQHLRSKSEEAKKILSTRDKTNVILSYSGQTASIEITRVKFEEI
                     TSHLLSKTKHIMEFVLEDANLTWNSIDKILLIGGSSRMKAVENMILEATGKKPSKEIH
                     PDEAVALGAAFQASLVGTEKGISARNNNLPIVLIQDVNSHSMGIVAYNPENNSSYNSI
                     VLKKDTPIPNKVSDIFRTIDNEQTELFIQVTEGECADLEDVYIIGEAKLKIPPYPAGA
                     PIEVFFCYDADGIVHINVFDLTGNKSLGEIHIKRTANLDESQILIMKNKISGLSVN"
     gene            complement(4946..5359)
                     /locus_tag="PK218_09970"
     CDS             complement(4946..5359)
                     /locus_tag="PK218_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98876.1"
                     /translation="MSLRSSHTHCQSPTIQRLNQSFAKECACHTQEEKLKISHPLENK
                     KYPNAQADTRHISNLTMIRKVNNDNGLQEQKTERQLVTGGIFYCRDSAGFSVSASNKL
                     SCNLTGQCFEIGNKNIPPKRYKQAEKKRETTKYEY"
     gene            complement(5399..6061)
                     /locus_tag="PK218_09975"
     CDS             complement(5399..6061)
                     /locus_tag="PK218_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98877.1"
                     /translation="MQTLDWKPKEHTPRALLIGHDPRLQISDTQAEYVLFANYFFHNT
                     IKDRAFKSKYGLAASAFNQITHITNGKIKQEEIYITNLCNSSLPHAPKGKTVYIPKNK
                     AIAGVENIKRIIEENPSIEYIFPMSLQVNYWLQKLGMYFSSNEFVEKSTPKAIGIQNE
                     PPYYEPIGKSPFLLICGNRYRVTGGTQYVIPILHAKCFPLNKQFKAYEPAYDNIRNFF
                     RI"
     gene            complement(6073..>6513)
                     /locus_tag="PK218_09980"
     CDS             complement(6073..>6513)
                     /locus_tag="PK218_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98878.1"
                     /translation="DYKASAVYLRTEFERIVKTICDKQKIPVCYHKNQKETTSEDFWT
                     AIESQTDLDPTLIHEITIHRGVVMNPFSHYDLEKPEFVKELEDTIKSIEKLKAIDPKS
                     LKKITIEQLKKEIIELKKEPADLKIKLQEKEDLVRELGRKLKEK"
BASE COUNT         1846 a         1209 c         1016 g         2442 t
ORIGIN      
        1 ttgggtattg ccgaaggcgg ggatttttag cacaaaagtt caatcgaaga acgaaagttg
       61 aacctttcac aaatgcccaa tagaagccgt tcagccccgc ttttggcaat accatgttgt
      121 acgacgtagt tatgttttta attttagttc ggttaaatat tttcgtatgt ctttttctaa
      181 tttatcaggg tatttataat ctagtttttc agataattct gttcctattt cagaaactaa
      241 atcaaccatt gaaaatagag cagtccaatt gtcttctata ttacttcctg aaaatgtgtt
      301 ttctgttttt gcccacattt cttggtttag atactttttg aatagccttc cgtatttatt
      361 ggttgttacg ttccaattgt gttcacttgc aatgaaccat tcaattaagg gaactaaata
      421 tccggtgcga attactgttt ctgacatata ttttgcatag aatatttcgt ctctcgcaag
      481 acatttagcc acataagtcg tgtcccacca aaagtcattg agtaaattat taaattcttt
      541 ttcagtcggt tttttgataa tagaaacttg ataagtaggt ttcaacattt gggttgtaat
      601 accatctttg tcaatcagaa ttttatagcc aatgtcccaa tcttcgggta attctttttg
      661 ttcagtttct tgtatgaaat ttaatttgct ataaagttta aaatctgctt ttactccatc
      721 ctcatacaga accattttca ttgcgtgttt attatcaaat gcgttttcgt cctcttcaat
      781 catcgcaatt ggatttccga agttaagcgt ccagcttttg tctgaaatat agtttgcgtt
      841 attctcaaaa ataaactcaa tgtctaaatc gctaaattcg tcaacaggtg ctaaaggatt
      901 tacgagtgaa cttgtcaaaa gtaggactct gacatcttca ttttttttcg cccactcaat
      961 aattgatttt agtttttcgt ctctaacgtt cattttgtgc ggttcggttt tactatgtcg
     1021 tacaacggtt tcggtatatg aaaagttggg gcacttcgga gcacgtcact gtcaaaccga
     1081 cacaaagttt gaagcgagct acaacccttg atttcagtag tatctgccca attttttata
     1141 taccgtgtta caagctggcc gttcatcttc agtcatataa tttacaacga aaacattttt
     1201 caaaattaca aatctttctg aaaataaaag ttttagagcg atgatttata ttattctgag
     1261 taatcagttt tttccataag cgatacttga tagggtaatt tgcatatata cagcaccgcc
     1321 aattgaacct acaattaaac caatagctaa gataattgat aaaacaatta gttttttcat
     1381 tataaattca atgaataatt tatacaaatt gtcctcaatt acaatcatat ttgagaaaaa
     1441 cgagttaatc ggcttataaa caattgaaaa accccaatag ccactccata caaaataacc
     1501 aataagaatt aatgatagaa atggattatc atatacatcg taacttgagc ctattattaa
     1561 gcctaaacca gcgaacacat agccgattag aagttttttc cgtgcttctt cttttgaaat
     1621 catataatca ttttaaaaat taaggggcaa atcaaaaaat gaaatgcccc tttaacagaa
     1681 atcatttgtc ataaagataa gcctctgctt tgttcattgc ttcgcgagca gagcttgctt
     1741 tgcctacact tgtccagcct gcccaactaa catatacatc tctacttgct tggtcccatt
     1801 taacatcaaa tgattttcta gatttgcctg atttgactga acctattctt tcagctgcca
     1861 tttttacctc ctttttttaa ttgtttgaca ataccttttt gttaatcttg taaccttctt
     1921 tgaaaccaaa gaaaatccat aatgcccaaa ctgccaatgc tatgagtatt aacgctgctt
     1981 cccctgatgc aaaaagtccg atacctccaa taccaagtat taatacccat tttattgtag
     2041 agccaatcga acgaccccaa tgtttactta aactccaatt tgcaacgtct aggttctttc
     2101 taataatagc ttttaattcg tcatcttcaa accttaaggc aagtgctttg gtaaaatatt
     2161 tcagggcgaa ttcagatgct tccttactta ctaaataata tgaaccatca tttgactcaa
     2221 ccatatattt tttcaaaacc aaatcattat acgctaatgc taaaaaccaa gtgtaagtag
     2281 tgttatctgg ttcttcactt acacaagatt ctaatatctg aatcgcatct tcatacatct
     2341 ctacacgtaa atagacttga ccaattgctg ccctgtaaac tgtaactttg gggtcaatct
     2401 ttgatgcttt ttggtaatta tcaacagcat cttgccatct ttgttgagat tcataaaccg
     2461 aacccaagtc aaaatagtat tcagagttat ttggacgcaa cttgattgct tttttgtatt
     2521 cgtaaattga gtcctcaatt tcaccccagc gatattttgc ctgtgcaagc aaagcccatg
     2581 cctctggatt atctccttgt ttttcagtag cttttgtgcc aatataaata gcatcagcaa
     2641 tattgtcact tattaatgct tcccaacctt gtttaacaag ttcgtctgca actttcaatt
     2701 ctttctcatc aactgttcgt tcatcagttt gagaagatgt tttaagtttt ttgtcatact
     2761 ctgacctttt cgatgaatct aataaaatct tttcagcttc atcaatctgt tttactttca
     2821 cttcggcttc ctgtcttctt tcgagttggg gagcattcgt tttttgtagc caaagacgtc
     2881 ttttgtcctt tatggctacc ttaatttctt cagactgtgc agtcattggc acgtcaagaa
     2941 ttttgtaata atcaatcata atatttaaaa tttaaggtta aaaaaaaagg aaatctatta
     3001 gtttacacta agtccactaa ttttattttt cattatcaat atttgacttt cgtcaagatt
     3061 tgcagttctt tttatatgga tttctccaag tgatttatta ccagttaaat caaaaacatt
     3121 aatatgcaca ataccatcgg catcataaca aaaaaatact tctattggtg caccagcagg
     3181 atatggcgga attttcaact ttgcctcgcc aataatataa acatcttcta agtcagcaca
     3241 ttcaccttct gtaacctgaa taaatagttc tgtttgttca ttatcaatag ttctgaaaat
     3301 atcgctcact ttattaggaa tcggagtgtc tttttttagt acaattgaat tataggatga
     3361 attattttca gggttatatg caactatgcc catgctatgc gagttgacat cctgtattaa
     3421 tactatgggt agattattat tgcgtgcaga tatgcctttt tcagtaccaa ccaaactagc
     3481 ttgaaatgct gctcctaatg caacagcttc gtcagggtgt atctctttag atggtttttt
     3541 acctgttgct tctaaaatca tattttcaac tgctttcatt cgtgaagaac cacctattag
     3601 caatatttta tctatggaat tccatgttag atttgcatct tccaaaacaa attccattat
     3661 atgcttagtt ttacttaata aatgtgaagt tatttcttca aatttaaccc ttgttatttc
     3721 aatggatgct gtttgtcctg aatatgatag aataacgttt gttttatctc ttgtggataa
     3781 aattttcttt gcttcttcgg atttactcct tagatgttgc tctgattcag gagtgtcaaa
     3841 taaatcagtt tttgttttgt ttttaaactc ctcatttaag tagttcataa tttcattatc
     3901 ccagtcaaaa ccacctaagt ttttgtcacc accagttgat aaaacattaa cttctccaga
     3961 tttaatattc attattgtta catcaaaagt cccacctcct aaatcataaa caagtaatgt
     4021 ttgtggttca ttgaatctgt cgtaactgta tgcaagagct gcagccgttg gttcgtttat
     4081 gattcttaaa acatttaaac ctgcaatatt tccagcatcc tgagttgatt ttctctgagc
     4141 atcattaaaa tatgcaggta cggtaataac tgcaccagca atagtgtcgt ttaaatattc
     4201 ttctgcatcc tctttgagtc gttttaatat tattgctgaa atttcttccg atgtatattc
     4261 atctcccttt tctgtgatga atttgaatga aggatttccc atttgtctct taacaaattg
     4321 gactacattt aatggttcca ttattgagga actttttgct gtaaccccaa caattggact
     4381 cccatcttca aaaaatacaa cagaaggcat tatacgctct ccatctcgat tgggaattat
     4441 ttcaggtttt cctgactcat taataaatgc aactgctgaa aatgttgtgc ctaagtcgat
     4501 tccgattact ttactcatat ttttaatttt taagttatac aaaaaaaaca gcgtggccag
     4561 atgcctaccg ctgtcgaggt tctaccaaaa cccactgaac aagtaaagca acgaccacgc
     4621 cttgacgtgg cgttttgctt actatcctgt tcagttttag aaatttggta gatttcgaca
     4681 acagaattct gctaaacgct atattttaaa attcatttat gtttacatac tcttattcat
     4741 ttagttagat attaaaaaag gtttgactgc aaaattaaca ttattaataa tatgcaaata
     4801 cttattggta aaatattgat tcttaacgca ctttagatgt attgcaaatt gttttaaaat
     4861 tatatgcaaa ttcatctttt tataaagagc gttggctaaa acttttattt tcattatttc
     4921 tttcaaacta acatatgttt tcgtttcaat attcatattt cgttgtctct cttttttttt
     4981 cggcttgctt gtaacgtttt ggcggtatgt ttttgttgcc gatttcgaag cactgtcctg
     5041 tcaagttaca cgaaagttta ttagatgcag aaacgctgaa acccgcacta tctcggcaat
     5101 aaaatatacc gcctgttacc agctggcgtt ctgtcttctg ttcttgtaaa ccattgtcat
     5161 tattgacttt tcgtatcatt gtcaagttgc ttatatgtct tgtgtcggct tgtgcgtttg
     5221 ggtatttttt attttctaag gggtgggaga tttttaattt ttcctcttgg gtatggcagg
     5281 cacactcttt ggcaaagctt tggtttaagc gctggatagt ggggctttga caatgtgtgt
     5341 gactgctgcg taggctcatt ttaattttct ttaagttttc ttttaatctt cattcaattc
     5401 agattctaaa aaaattacgt atattatcgt atgctggttc atacgcttta aactgtttat
     5461 tgagaggaaa acattttgcg tgaagaattg ggattacata ttgtgttcct ccggtaaccc
     5521 gatacctatt tccacaaatc aacagaaaag gacttttccc tattggttcg taatagggtg
     5581 gttcgttttg aattccaatt gcttttgggg tcgatttttc gacaaattca ttactcgaaa
     5641 agtacatccc taatttttgc agccagtaat ttacctgaag cgacatcgga aaaatgtatt
     5701 caatgcttgg gttctcctca attatcctct ttatattttc aacacctgca attgccttgt
     5761 tttttgggat ataaacggtc ttaccttttg gagcatgggg caaagatgaa ttgcaaagat
     5821 ttgtaatgta tatttcttct tgtttgattt taccgtttgt aatatgagtt atctgattaa
     5881 aagccgatgc agccaaacca tatttacttt taaatgccct gtccttgata gtattgtgga
     5941 agaaataatt tgcaaacaat acatattctg cctgtgtgtc tgaaatttgc aaacgagggt
     6001 cgtggccgat tagtaaggcc cttggggtat gttcttttgg tttccagtcg agtgtttgca
     6061 tctatctttg aattatttct cttttaattt tctaccaagt tctctaacca agtcctcttt
     6121 ctcctgtagt tttattttca aatcagcagg ttctttcttt aattcaatta tttctttttt
     6181 cagttgttca attgtaatct ttttgaggct cttgggatcg attgctttca gtttctcaat
     6241 agatttaatt gtatcttcca attcctttac aaactccggt ttttcaaggt cgtaatggct
     6301 gaaaggattc attacaactc cacgatgaat tgtaatttca tgaattaatg tagggtcaag
     6361 gtcagtctgg gattctatgg ctgtccaaaa gtcttcgctt gttgtctctt tttggttttt
     6421 gtggtagcat acagggattt tctgcttatc acaaatggtc tttacaattc tttcaaattc
     6481 tgttctcaga taaacggctg atgctttata gtc
//
LOCUS       DAOOBH010000116         6490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_14009_length_6490_cov_4.440404, whole genome shotgun sequence.
ACCESSION   DAOOBH010000116 DAOOBH010000000
VERSION     DAOOBH010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14009_length_6490_cov_4.440404"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PK218_09985"
     CDS             <1..866
                     /locus_tag="PK218_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HPW98879.1"
                     /translation="FFPTGTMANQTAVKLNTNPGDQIICDKWTHIHLYEAGGASSNSG
                     VNFNLLDGNRGMITAEQVKEGINDPEFYHAPLTKMVGIENTTNKGGGACYEMEELIKI
                     KQVCIDHNLKFHLDGARLWNALIAKKQHPKQFGALFDTISVCFSKGLGCPIGSVLLSD
                     AETMKKALRIRKIFGGNMRQSGYLAAAGIYALQNNINRLEEDHNRAKELGKQLQQCSW
                     VKVVEPIETNIIVFSPIDTVSDQQIMDKLKQKGISISLMSKGKLRIVTHLDYRQIMHE
                     YVLEALSKLAF"
     gene            complement(863..1594)
                     /locus_tag="PK218_09990"
     CDS             complement(863..1594)
                     /locus_tag="PK218_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPW98880.1"
                     /translation="MNNINYLFLVFALLAEIIGTIGGFGSSVFFVPLGNFYFDFHTVL
                     GLTAVFHLSSNLSKIILFKKGLDKNLLLYIGLPSVIFVIIGGIVSRYLDTVLLELLLG
                     IFLVVLSLLFLVKQKLVIEPNKENSITGGILSGFSAGLLGTGGAIRGLTMAAYNLEKS
                     VFIATSAAIDMMIDATRTVVYYENGYITKDIIIYIPFLFVIGLVGTYLGKRILHYIPQ
                     DKFKNLSLVLILIIGLITILKQFLN"
     gene            complement(1594..1917)
                     /locus_tag="PK218_09995"
     CDS             complement(1594..1917)
                     /locus_tag="PK218_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPW98881.1"
                     /translation="MFGDMMNMMGKLKETQQKIEETKQRLDTVLIDEQSNDGLLKVTL
                     TANRKIKSISIDNNLLEDKEQLEDYLILVLNKAIEKATNVNETELAAVAKQGMPNIPG
                     MDLFK"
     gene            2046..4103
                     /locus_tag="PK218_10000"
     CDS             2046..4103
                     /locus_tag="PK218_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPW98882.1"
                     /translation="MAKKIVPPIAKIIPKTLEKHGDVRIDNYYWLNDRENPEVIDYLN
                     KENDYYQKSTAHTKQFQAILFEEMKARIKEDDQSVPYFYNGYWYITRFEIGKGYPIYA
                     RKKQSLEAEEEILFDCNEMAKDHAYFKLTGLSVSDDNKWISFGVDTVSRRQYTIQIKN
                     LETNEILPLKIENTTGGATWANDNKTFFYSRKDEQTLRPDKIFKHELDSSPENDILVY
                     FEKDETFDVSVYKSKSRKYIMINSDSTMTSEYRYLLASKPNGKFKVFQKRTRGLEYSI
                     SHFEDHFYIVTNKDKATNFKLMKTPEDATSKENWVDLIPHREQVLVEAIDIFKDYLVV
                     SERFNGLNQLKIMPWKGKEYYLPFDSETYMAYTTTNIDFDTEILRYSYQSLATPSSVI
                     DFNMRTKEKTVLKEQEVLGGTYDKSNYTEKRIWATAKDGIKVPISMIYKNGVEQNGKN
                     PTLLYAYGSYGVTMDCHFSSVRLSLLDRGFIFAIAHVRGGEDLGRNWYDNGKLLKKKN
                     TFTDFIACSEFLIKEKYTSNEHLYAEGGSAGGLLMGAIINMAPNLYKGVIAQVPFVDV
                     VTTMLDGSIPLTTSEYDEWGNPNQKKYYDYMKSYSPYDNVVAQDYPNIYISTGLHDSQ
                     VQYWEPAKWIAKLRVLKTNNNQLYLDVNMDAGHGGASGRFEALKEIAKEYCFLLDLQG
                     IQG"
     gene            4185..5225
                     /locus_tag="PK218_10005"
     CDS             4185..5225
                     /locus_tag="PK218_10005"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HPW98883.1"
                     /translation="MKEDIKAYDNVLGLIGNTPLIKLNKVTENIEGNFYAKVEAFNPG
                     HSTKDRIALYIIEEAERQGVLTPGDTIIETTSGNTGFSLAMVSIIKGYNCILAVSSKS
                     SMDKIDMLRSLGAKVYVCPAHVSADDERSYYNVAKRLHEETKGSVYINQYFNQLNIDA
                     HYKTTGPEIWEQTNGKITHLVACSGTGGTISGAAKFLKEKNPNIRILGVDAFGSVLKK
                     YHETKEFDSNEIYPYRIEGLGKNLIPTATDFDVIDHFMKVTDEESAHTTREIAKKEGL
                     FVGYTSGAVMQAIKQYAEEGEFDENSNVIAIFPDHGSRYMSKVYSDDWMNEQGFFDTK
                     NQEEAQKIEIIK"
     gene            5520..6353
                     /locus_tag="PK218_10010"
     CDS             5520..6353
                     /locus_tag="PK218_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98884.1"
                     /translation="MNVKKLIFGLTLSMFIFSCNKDDSTSSNSLDASQARTSVEIDKL
                     TDDVSYVIENQIDIQLSDSGKMTEIPESLLPLCATVTTVITGNTWTRTIDFGTEGCTM
                     PNGNVYKGVITISKILTSNPLSRTFTITFENFYSNARLIEGTKTIVRTLTTVAPIHPT
                     STMTMNITVTFPNGESYERTGNRVSEMIEGFGTAIWSDNIFSVTGNWSTTRLNWSQTT
                     TITTPLVFKMNCQYGLVQGEIQIVRNNNSAVINYGDGTCDNQATISINGSAPATFTFG
                     N"
BASE COUNT         2351 a          912 c         1112 g         2115 t
ORIGIN      
        1 tgttttttcc aacaggaact atggccaatc aaacggcggt taaattaaat accaatcctg
       61 gtgaccaaat tatttgtgat aaatggacac acattcattt gtatgaagct ggtggcgcat
      121 cgtctaatag tggtgtaaat tttaatctat tagatggaaa tagaggaatg attactgccg
      181 agcaagtaaa agaagggata aatgacccag agttttacca tgcaccttta actaaaatgg
      241 tgggaataga aaatacaaca aataaaggcg gcggtgcttg ctatgaaatg gaggaactta
      301 tcaaaataaa gcaagtatgt attgaccata atttaaaatt tcatcttgat ggtgcacgat
      361 tgtggaacgc tttgattgct aaaaaacaac atccaaaaca atttggtgca ttgtttgata
      421 ccatttcggt ttgtttctct aaaggattgg gttgtccaat tggttcggta ttattatcgg
      481 atgctgaaac gatgaaaaaa gcgttacgta tccgaaaaat atttggtgga aacatgcgcc
      541 aatcgggcta tttggctgct gcaggtatat atgcgttaca aaataacatc aataggttag
      601 aagaagatca taatcgagcc aaagaattag gcaaacaatt gcaacaatgt tcttgggtaa
      661 aagtagtgga accaatagaa acgaacatca tagtattttc gccaattgat accgtttctg
      721 accagcagat tatggataaa ttaaaacaaa aaggcatttc aatcagtttg atgtcaaaag
      781 gaaaattgcg aattgtaacg catttagact accgacaaat aatgcacgaa tatgttttag
      841 aagccttgag caagttggcg ttttaattca gaaattgttt taaaattgta attaaaccaa
      901 tgattaaaat taataccaat gataggtttt tgaatttgtc ttgaggaata taatgtagaa
      961 ttctttttcc taagtaagta cctactaatc caataacaaa caagaaagga atatatatga
     1021 taatatcttt ggtaatatat ccattttcat agtaaaccac ggttcttgta gcatctatca
     1081 tcatatcgat tgctgctgat gtagcaataa atacactttt ttcaagattg taagcagcca
     1141 tggttaatcc gcggatggca ccgccagttc cgagtaagcc agctgaaaac cctgataaaa
     1201 ttccacctgt tattgagttt tctttattgg gttcaatgac taatttttgt ttgactaaaa
     1261 acaataaact tagtacaact aaaaaaatcc ctaacaatag ttctaataat actgtatcta
     1321 agtagcgtga aactatcccg ccaattataa caaaaataac agaaggcaat cctatgtata
     1381 ataatagatt tttatcaaga ccttttttaa aaagaattat tttactcaaa ttgcttgata
     1441 agtgaaacac agcagttaat ccgagtacgg tatgaaaatc aaaataaaaa tttcctaatg
     1501 gtacaaaaaa caccgaagaa ccaaaaccac caatagttcc aataatttct gccaataaag
     1561 cgaaaactag aaaaaggtag ttgatgttat tcattattta aataaatcca ttccaggaat
     1621 atttggcatt ccttgttttg caaccgccgc aagctcagtt tcattaacat tggtagcttt
     1681 ttctatggct ttgtttagta ctaaaattag gtaatcttct agttgctctt tgtcttcaag
     1741 caagttgttg tctatagaaa ttgatttaat ctttcggttg gctgttaagg ttacttttag
     1801 taaaccgtcg ttgctttgtt cgtctattaa aacggtgtct aatcgttgct tagtttcttc
     1861 aattttttgt tgggtttctt ttaattttcc catcatgttc atcatgtctc caaacatagt
     1921 tgtatcaatt taaattagtt ggtaaaaata caaattcaac tgttaatttt tattggataa
     1981 cttaataaaa aattaactat ttcaatagag tattattcta attttacatg cttaaataaa
     2041 tcaaaatggc aaaaaagata gttccgccaa tcgctaaaat tattcctaaa acgctagaaa
     2101 aacatggtga tgttcgcata gataattact attggctgaa tgatagagaa aatccagaag
     2161 tgattgatta tttgaataaa gaaaacgact attaccaaaa atcaacagct cataccaaac
     2221 agtttcaagc tattttgttt gaagaaatga aggcaaggat taaggaagat gatcaatcgg
     2281 ttccttattt ttataatgga tattggtata tcactcgttt tgaaatagga aaaggttacc
     2341 caatttatgc tcgaaaaaag caatcattag aagctgagga agaaatactt tttgattgta
     2401 atgaaatggc caaagaccac gcttatttta aattaacagg attgagtgtt agtgatgaca
     2461 acaaatggat ttcttttggt gtcgataccg tttcaagacg tcaatatact attcaaatta
     2521 aaaatttgga aactaatgaa attcttccct taaaaattga aaacactact ggtggtgcaa
     2581 cttgggcaaa tgataataaa acgttttttt actcaagaaa agacgagcaa actcttagac
     2641 ctgataaaat attcaaacac gaattagatt caagtccaga gaatgatatt ttggtttatt
     2701 ttgaaaaaga tgaaactttt gatgtttcgg tttataaatc aaaatcgcga aagtacatta
     2761 tgattaattc cgatagtact atgactagtg aatatcggta tttattagca tcaaaaccaa
     2821 atggaaaatt caaagtgttt caaaaaagaa ctcgtggttt agaatattcg atttcgcatt
     2881 ttgaagacca tttttatatt gtaactaata aagataaagc aaccaatttt aaattgatga
     2941 aaactcctga agacgcaaca tcaaaagaaa attgggttga tttaattcca catagagaac
     3001 aagttttagt agaagcaatc gatattttta aagattattt agtggtttct gaacggttta
     3061 atggtctgaa tcaattgaaa attatgccat ggaaagggaa agaatattat ttgccatttg
     3121 atagtgaaac ttatatggca tataccacaa ccaatatcga ctttgatacc gaaattctcc
     3181 gttatagtta tcaatcatta gcaactccaa gctcagttat tgatttcaat atgcgaacca
     3241 aagaaaaaac agtattgaaa gaacaagaag ttcttggtgg aacatatgac aaatcaaatt
     3301 atacagaaaa aagaatttgg gcaacagcaa aagatggaat aaaagttcct atttcaatga
     3361 tttataaaaa tggagtagag cagaacggta aaaatccgac tcttttgtat gcttatggtt
     3421 cctatggtgt tacgatggat tgtcattttt catccgttcg attgtcattg ttagacagag
     3481 gatttatttt tgccattgct catgttcgtg gcggagaaga tttaggaaga aattggtatg
     3541 acaatggaaa attattgaaa aagaaaaaca catttactga ttttattgct tgttcggagt
     3601 ttttaataaa agaaaaatac acttcaaacg aacatcttta tgccgaaggt ggttcggctg
     3661 gaggattatt gatgggtgca attattaata tggcaccaaa tttgtataaa ggagtaattg
     3721 cgcaagtccc atttgtagat gtagtgacaa caatgcttga tggtagtatt ccgcttacaa
     3781 caagtgaata tgatgaatgg ggaaatccta atcagaaaaa atattatgat tatatgaaaa
     3841 gctactcacc gtatgataat gtagtagctc aggactatcc aaatatttat atatcaactg
     3901 gtttgcacga ttctcaagtg caatattggg aacctgcaaa atggattgct aagctaagag
     3961 ttttgaaaac taataataat caattatatt tggatgttaa tatggatgct ggtcacggtg
     4021 gtgcatctgg acggtttgaa gcattaaaag aaattgcaaa agaatattgt tttttattag
     4081 atttacaagg aatacaaggc tagttaaaat tttttttatt aaatttgcaa cgttttgggg
     4141 ttttcaaaca tgtcccaaaa tttaaagctt gactaattta ttttatgaaa gaagatataa
     4201 aagcttacga taatgtacta ggattaatag gtaatacacc attaattaag cttaacaaag
     4261 taactgaaaa tatagaagga aatttctatg ctaaagttga agcatttaat ccaggacact
     4321 ctactaaaga cagaattgca ttatacatta ttgaagaagc tgaaagacaa ggagttttaa
     4381 ctcctggaga taccattatt gaaactactt cgggtaatac tgggtttagc ttggcaatgg
     4441 taagcatcat caaaggttat aattgtattt tggcagtaag ttctaaatct tctatggata
     4501 aaatagatat gcttagaagt cttggtgcta aagtttacgt ctgtccagca catgtttctg
     4561 cagatgacga gcgttcgtat tacaatgttg ccaagcgttt gcacgaggaa accaaaggtt
     4621 ctgtttatat taatcaatat tttaatcaat taaatattga tgctcattat aaaactacag
     4681 gtccagaaat atgggaacaa actaatggaa aaataacaca tcttgtagct tgtagcggaa
     4741 caggaggaac tatttctggt gcggcaaaat tcttgaaaga aaaaaatccg aatattagaa
     4801 ttttaggcgt tgatgctttt ggttcggtgc tgaaaaaata tcatgaaaca aaagagtttg
     4861 actcaaatga aatctatcca taccgaatag aaggtttggg taaaaactta attccaacag
     4921 caaccgattt tgatgtaatt gatcatttca tgaaagttac agatgaagaa agtgcacata
     4981 caactagaga aatagctaaa aaagaaggtt tgtttgtagg ttatacttca ggtgcagtaa
     5041 tgcaagccat taaacaatat gcagaagaag gtgaatttga tgaaaacagc aacgtaatag
     5101 ctatttttcc tgatcatggc tctagatata tgagtaaagt atatagcgat gattggatga
     5161 acgaacaagg tttctttgat actaaaaatc aagaagaagc tcaaaaaata gaaatcatta
     5221 aataattttt tgaagaatta cttaagtttt agtttttttt aaaattatta attattctat
     5281 tatataatca attatttttt atagtataat tctcgccttt ataagtagtc attttttaat
     5341 agaaatgaag tatggttaat ctatacttag cttattatca aagtgtttta gttttttaac
     5401 tatttttttt ataactattt cttgctttaa tttattatat tttgaaaaaa atagtcatgt
     5461 tgattaaacc tttttattaa ttcaatatct aatttcaaaa ccataaaaaa taaatgatta
     5521 tgaatgttaa aaaattaatt tttggattga cattatctat gtttatcttt agttgtaaca
     5581 aagacgattc tacttcaagt aattctttag atgcatctca agcaagaacc agtgttgaaa
     5641 ttgacaaact aactgatgat gtttcttatg ttattgaaaa tcaaattgat attcaattaa
     5701 gtgattctgg gaaaatgact gaaattcccg aaagcctact tccactttgt gcaactgtaa
     5761 caactgtaat tactggtaat acatggacaa gaaccataga ttttggaaca gaaggttgta
     5821 ctatgccaaa tggaaatgta tataaaggtg tgattactat ttccaaaata cttactagca
     5881 atcctttgtc tagaacattt actataactt ttgaaaactt ttatagtaat gcacgattaa
     5941 tagagggtac aaaaactatt gttagaacat taacaacagt tgcaccaatt catccaacaa
     6001 gcacaatgac aatgaatatt accgttactt ttccaaatgg agaatcctat gaaagaactg
     6061 gaaatagagt ttctgaaatg attgaaggat ttggaactgc aatttggagt gataatattt
     6121 tcagtgtaac tggaaattgg tcaacaacaa gattaaattg gagccaaact acaactatta
     6181 caacaccatt agtttttaaa atgaattgtc agtatggatt agttcaagga gaaattcaaa
     6241 ttgttagaaa taacaattcg gcagttatta attacggaga tggcacttgt gataatcaag
     6301 ctacaatttc tataaacgga agtgctcctg ctactttcac tttcggaaat taatataatt
     6361 tacacactta ctagaaaacg tttcaaaaat ttgaaacgtt ttttttttat gatttatttg
     6421 ccaattgacc acaagcagca tcaatatctt tttctctact gcgtcgcact tttgccacta
     6481 tattgttttt
//
LOCUS       DAOOBH010000117         6437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_14155_length_6437_cov_4.175180, whole genome shotgun sequence.
ACCESSION   DAOOBH010000117 DAOOBH010000000
VERSION     DAOOBH010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14155_length_6437_cov_4.175180"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PK218_10015"
     CDS             <1..636
                     /locus_tag="PK218_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPW98885.1"
                     /translation="LGSSCIYPKLAPQPLQEECLLTSSLEPTNEWYAIAKISGVKSCE
                     AIRKQFGKDFVSLMPTNLYGPNDNFDLQTSHVLPAMMRKFHEAKINGNQAVTLWGSGA
                     PMREFLHVHDMADAVVHAFENPLDEHLYNIGSGVDLTIKSLAELIQKTVGHQGEIIWD
                     SSKPDGTPRKLMDVSKMKNKGWSAKISLEEGIATTYQWFLEHEHSFKEVKL"
     gene            655..1809
                     /gene="gmd"
                     /locus_tag="PK218_10020"
     CDS             655..1809
                     /gene="gmd"
                     /locus_tag="PK218_10020"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPW98886.1"
                     /translation="MKTALITGITGQDGAYLAELLLEKGYMVHGIKRRSSLFNTDRID
                     HLYQDPHVTDIRLKLHYGDMTDSMNLTRIIQEVQPDEIYNLAAMSHVKVSFDIPEYTA
                     NADGVGTLRLLEAVRLLGLQHKTKIYQASTSELYGLVQAVPQSETTPFYPRSPYAVAK
                     IYAYWITVNYREAYNMFACNGILFNHESPLRGETFVTRKITRAVAKIALGLQNDLFMG
                     NLDAQRDWGHAKDYVEAMWRILQQDVAEDYVIATGITTRVRDFIRMAFDEVGLKIKFE
                     GEGIDEKGILEAIDSEKYKNALGKDFSIENITLPKIGAEIVKVDPQYFRPTEVDLLIG
                     DPSKSKTKLGWQPKYDLPMLVEEMVAADVKLFQKDIHLVQGGHKILKQAE"
     gene            1831..2991
                     /locus_tag="PK218_10025"
     CDS             1831..2991
                     /locus_tag="PK218_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW98887.1"
                     /translation="MEKIKDRKIVVINQASNYLTVGFCNAFSAQFESVSLITGSIHIQ
                     GEELNKNVKVTPINKWVERPASKKFWSYIKACLKIYWLLLTKYRKHEVFFVSIPPMGY
                     LLNLLVSNRFSILVWDVYPDVFKITGMKENHFLYKTWASLNKKSFKKSFRLFTIGNKM
                     ADLLEVYVPRTKMIIQPIWSIFQANERVLKTENPFIKEHNLENKFIIQYSGNIGLTHK
                     VEIVVEIAELLKDNKDIIFQIIGRGPRVPALQKMVEEKNLPNCMFLPFQSDAMFPFSL
                     SAADLGIVILDELTSKGSVPSKSYNLMSYGIPSIYIAGEDSELHDYANQFKAAACFTE
                     RELQAAKNFILDLASNKQKWEMMSQNALETSKLFRRDNAYKFVELYLNSSFD"
     gene            2984..4120
                     /locus_tag="PK218_10030"
     CDS             2984..4120
                     /locus_tag="PK218_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98888.1"
                     /translation="MIKKFAHTVFSNLKNALGWKTNRKIIVFSVDDYGNVRLNSTEAR
                     KNMDEVGMKIYSRFDALDTLETKQDLEQLYEVLESVKDKNGNPAIFTPFTLPCNINFE
                     KMEAEDFQEFHFEPLPETYKKLAIQQPEAYQNAWETWQKGIEKGILKPQFHGREHLNL
                     KIFNEKLKTRDTQLLVALKNKSYTSISDDSYPTMSCFAAFDFWDVKENDGLKPLLIDG
                     LNWFEKIFGYQSTYFTPPVFNIHHSLFQTLKDKGIRFIDLALMRSEHQGFGKYKKAFN
                     YTGKKANNGLTIMVRNVVFEPTEDRGIDWVQFTMKQIDTAFRWNKPAIISSHRVNFCG
                     HIDPKNREKGLETLKKLLQEIVVKWPNVEFMSADTLGELVLNKK"
     gene            4122..5126
                     /locus_tag="PK218_10035"
     CDS             4122..5126
                     /locus_tag="PK218_10035"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HPW98889.1"
                     /translation="MKRILYLGYYLKNLDWKKLQSFLEYASKKTKKTKISLLGDAFYS
                     VFKYNISILEYFQFRFFEQNTEQRKTWAGTGYMFEYQLTMNPKKERIILDDKRRFFKE
                     YQKYFVHQVASLEEMKNNPEVIQQLLNNPSGKIVFKVSDGKCGIAVQVAKAKDFDVKS
                     IVPFMEKNQFDMIEEFIVQHSELNKLSPSGVNTVRIFTQLDKNNEVILLGCRQRISVN
                     SSVDNMAAGNMAAVIDENSGKIIDAGIYSDITKNEEIIHPITNVSIIGFQVPFWNETI
                     KMVKEAAILHPQNRSIGWDIVITENGPGFIEGNHDWCKLLWQLPAKKGLKSLLKPHSK
                     "
     gene            5126..5731
                     /locus_tag="PK218_10040"
     CDS             5126..5731
                     /locus_tag="PK218_10040"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPW98890.1"
                     /translation="MYRFFFKRILDLITAIVVLILTLPITIVVYLILFKQNNGKVFFF
                     QERPGWHQKAFNIIKFKTMTDAKDSEGKLLPDNQRITKAGKVIRKLSIDELPQLLNVL
                     KGDMSLVGPRPLLFKYMPLYSVEQLRRHEVRPGITGWAQVNGRNSIAWTKKFEFDVYY
                     VDKLSFLLDIKILWFTFLKVIKTEGVNQTEDRPMLPFDGTN"
     gene            5740..6372
                     /locus_tag="PK218_10045"
     CDS             5740..6372
                     /locus_tag="PK218_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HPW98891.1"
                     /translation="MKKKLLIVGAGSVGKFVAYNIDNFTQEFEIIGFLDDDISKQNTE
                     ISGYFVIGSVEKLSEFSGKNVAIVWGIAFPEIKKRLLNEYKHLDFEYPNFISKKAWIS
                     NQVTFGKGCIVYPGNSINYETQVEDFVVINMNCAIGHNCIIGSFTSLSPGVNLGGNTT
                     IGDDVELGIGSATVQGISIGNNTKVGGQAMVAKSIASHQIVRGIPAKSIQ"
BASE COUNT         2335 a          887 c         1147 g         2068 t
ORIGIN      
        1 ttaggaagtt cgtgtattta tccaaaatta gctccacaac cgctacaaga agaatgttta
       61 ctaacttctt cattagagcc tacaaatgaa tggtatgcta ttgcaaaaat ttcgggcgta
      121 aagtcttgtg aagccattag aaaacaattt ggcaaagatt ttgtgagctt gatgccaacc
      181 aatttatatg gtccaaacga taattttgat ttgcaaacgt cacatgtttt accagcgatg
      241 atgcgcaaat ttcacgaagc aaaaataaac ggtaatcaag ccgtaacact atggggttca
      301 ggcgcaccta tgcgcgaatt tcttcatgtt catgatatgg cagacgcagt tgttcacgca
      361 tttgaaaatc cattagacga acacttatac aatattggtt caggagttga tttaaccata
      421 aaatcattgg cagaactgat tcaaaaaaca gtaggtcatc aaggtgaaat tatttgggat
      481 tcgtctaaac ctgatggaac tccacgaaag ctgatggacg tttctaaaat gaaaaataaa
      541 ggatggagtg caaaaatttc tttagaggaa ggaattgcca caacttacca atggtttttg
      601 gaacacgaac attcgttcaa agaagtaaaa ttataattat taaaaaataa atcaatgaaa
      661 actgcattaa taacgggcat tacaggacaa gacggagctt atttagccga gttgttactt
      721 gaaaaaggat atatggttca tggaattaaa agacgctctt ctttatttaa caccgataga
      781 attgaccatt tataccaaga cccacacgtt actgatattc gtttaaaatt acattatggc
      841 gatatgaccg attctatgaa tttgactaga atcattcaag aagtgcaacc ggacgaaatt
      901 tataatttgg ctgcaatgtc gcatgtaaaa gtaagttttg atattccaga atatactgcc
      961 aatgctgatg gtgttgggac attacggctt ttagaagcag tacgattatt gggtttacaa
     1021 cataaaacta aaatctatca agcttctacg tcagaattgt atggattagt gcaagcagtt
     1081 ccacaatcgg aaacaacacc tttttatccg cgttcgcctt atgcagtagc caaaatttat
     1141 gcctattgga taacagtaaa ctatcgtgaa gcctacaata tgtttgcttg caatgggatt
     1201 ttgttcaatc atgaatcacc attaagaggc gaaacttttg ttactagaaa aattactcga
     1261 gcagttgcca agatagcttt aggtttacaa aatgatttat tcatgggaaa tcttgatgca
     1321 cagagagatt ggggacatgc aaaagactat gttgaagcca tgtggcgcat tttgcagcaa
     1381 gatgtagctg aggattacgt aattgcaaca ggaattacca caagagtgcg tgattttata
     1441 agaatggctt ttgatgaagt tggattaaaa ataaaatttg aaggagaagg aattgatgaa
     1501 aaaggaattt tagaagctat cgattctgaa aaatataaaa atgcattagg aaaagacttt
     1561 tctattgaaa atattactct gccaaaaatt ggagctgaaa tcgtaaaagt agatcctcaa
     1621 tatttccgcc caacagaagt ggacttatta attggtgacc catcaaaatc taaaactaaa
     1681 ttaggttggc aacctaaata tgatttacca atgttagtag aagaaatggt tgctgctgat
     1741 gtcaaattat tccaaaaaga cattcactta gtacaaggcg gacataaaat tttaaaacaa
     1801 gctgaataat agttttagca aaatattcgc atggagaaaa taaaagatag aaagatagtt
     1861 gtcataaatc aggcatccaa ttatctgact gttggttttt gtaatgcctt ttctgctcaa
     1921 tttgaatcgg tgagtcttat tacaggaagt attcatattc aaggagaaga attaaacaaa
     1981 aatgtaaaag ttactccaat taataaatgg gtagaacgac cagcaagcaa aaaattttgg
     2041 tcgtatatta aagcttgttt aaaaatttat tggttgcttt taactaaata tagaaagcat
     2101 gaagtgtttt ttgtatcgat tccaccaatg ggttatttgc ttaatttatt ggtttccaat
     2161 cgtttcagta ttttggtttg ggatgtttat cctgatgttt ttaaaattac aggaatgaaa
     2221 gaaaatcatt ttttgtataa aacttgggct tctttaaata aaaaatcatt taaaaaatca
     2281 tttcgccttt ttactattgg taataaaatg gctgatttgc tagaggttta tgttccaaga
     2341 actaaaatga taatccaacc catttggtca atatttcaag caaatgaacg agttctaaaa
     2401 actgaaaatc catttattaa agagcataat ttagaaaata aattcattat tcaatattcg
     2461 gggaatattg gattaacgca taaagtggag atagttgttg aaattgccga acttttaaaa
     2521 gacaataaag atattatttt tcaaattata ggtcgtggtc caagagtgcc agctctgcaa
     2581 aaaatggtag aagaaaagaa cctacctaat tgtatgtttt taccatttca aagtgatgcg
     2641 atgtttcctt tttcactttc tgctgccgat ttaggaatcg taattctaga tgaattaact
     2701 tcaaaaggaa gtgttccaag taaatcatac aatttgatga gttacggcat tccatcaata
     2761 tatattgctg gagaagattc ggaattacac gattatgcta atcaatttaa agcagcagca
     2821 tgttttacag aaagagaatt acaagccgct aaaaacttta ttttagactt agcctctaac
     2881 aaacaaaaat gggaaatgat gtctcaaaat gctttggaaa cttccaaatt attccgaaga
     2941 gataatgctt ataaatttgt tgaactttac ttaaattctt cttttgatta aaaaatttgc
     3001 tcatactgtt ttctcaaatt taaaaaatgc tttaggttgg aaaaccaata gaaaaattat
     3061 tgttttttca gtagatgatt atggcaatgt gcgattaaat tcgaccgaag cacgtaaaaa
     3121 tatggatgaa gttggcatga aaatttattc tcgatttgat gctttagaca ctttagaaac
     3181 caaacaagat ttagaacaac tttatgaagt tttagaatca gtaaaagata aaaatggaaa
     3241 tccagctatt tttacaccat ttacgttacc ttgcaatatc aattttgaaa aaatggaagc
     3301 agaagatttc caagaatttc attttgaacc gttacccgaa acgtataaaa aattagccat
     3361 tcaacaaccc gaagcctatc aaaatgcatg ggaaacatgg caaaaaggaa ttgaaaaagg
     3421 aatattaaaa ccccaatttc atggtagaga acatttgaat ttaaaaattt ttaatgaaaa
     3481 attaaaaaca cgagatactc aattgttagt ggctttaaaa aacaaatcgt acactagtat
     3541 atcggatgat agttatccaa ccatgtcatg ttttgctgct ttcgattttt gggatgttaa
     3601 agaaaacgat ggattaaaac ccctattaat tgatggttta aattggtttg aaaaaatatt
     3661 tgggtatcaa agcacctatt ttacaccacc ggtttttaat attcatcatt cattatttca
     3721 aactttaaaa gataagggaa ttcgatttat agatttagct cttatgcgaa gcgaacatca
     3781 aggttttgga aagtataaaa aagcattcaa ttatacggga aaaaaagcga ataacggcct
     3841 aactatcatg gttcgaaatg tagtttttga gcctaccgaa gatagaggaa tagattgggt
     3901 tcaatttaca atgaaacaaa ttgataccgc tttcagatgg aataagcctg caataataag
     3961 ttcgcatcga gtgaattttt gtggtcacat tgatcctaaa aacagagaaa aaggattaga
     4021 aactttaaag aaattgcttc aagaaattgt tgttaaatgg cccaatgttg agtttatgtc
     4081 agctgatact ttaggtgaat tagtattgaa taaaaaataa aatgaagaga attttatact
     4141 taggttatta tttgaaaaat ttagattgga aaaaattgca atcgttttta gaatatgctt
     4201 caaaaaaaac taaaaaaact aagatatctc ttttaggcga tgcattttat agtgttttta
     4261 agtataacat ttcaatttta gaatattttc aatttcgttt ttttgaacaa aatactgaac
     4321 aacgaaaaac ttgggctgga acaggatata tgttcgaata tcaattaacc atgaatccta
     4381 aaaaggagcg tattatttta gatgataagc gtagattttt taaagaatat caaaagtatt
     4441 ttgtacatca agttgctagc ttagaggaaa tgaaaaataa tccagaagta attcagcaat
     4501 tgttgaataa tccatctgga aaaattgttt ttaaagtttc tgatggaaaa tgtggtattg
     4561 ctgttcaagt tgccaaagca aaggattttg atgtaaaatc aattgtacca tttatggaaa
     4621 aaaatcaatt tgacatgatt gaagaattta tcgttcaaca ttctgaattg aataaattgt
     4681 cgccttcagg agttaatact gtacgaatat ttacgcaatt agataaaaat aatgaagtga
     4741 ttttattggg ttgtcgtcaa cgaatttctg taaattcttc tgttgataac atggcagcag
     4801 gtaacatggc agcagtgatt gatgaaaatt cgggtaaaat tattgacgct ggtatttata
     4861 gcgatattac aaaaaatgaa gaaataattc atccaattac taacgtttct attattggtt
     4921 ttcaagtacc attttggaat gaaacaataa aaatggtcaa agaagccgct atattacatc
     4981 ctcaaaaccg ttctattggt tgggatatag tcatcacaga aaatggtcca ggatttattg
     5041 aaggaaatca tgattggtgt aaattattgt ggcagcttcc agctaaaaaa ggattaaagt
     5101 cactactaaa acctcatagt aagtaatgta tcgctttttt tttaaaagaa ttttagattt
     5161 aatcacagca attgtggttt tgattttaac attgccaatt acaattgtag tttatttaat
     5221 tttattcaaa caaaacaatg gaaaggtttt cttctttcaa gagcgacctg gttggcatca
     5281 aaaagctttt aatattatca agtttaaaac catgacggat gcaaaagata gtgaaggaaa
     5341 attattgcca gacaatcaaa gaattacaaa agcaggcaaa gttattcgta aattatctat
     5401 tgatgaatta cctcagttgc ttaatgtttt aaaaggcgat atgagtttgg ttggtcctag
     5461 accattgttg ttcaaataca tgccgctata ttctgtggaa caattgcgtc gtcatgaagt
     5521 tcgaccagga attacaggtt gggcacaagt aaatggaaga aattcaatag cttggaccaa
     5581 aaaatttgaa tttgacgttt attatgtaga taaattgtct tttttattag atataaaaat
     5641 tctttggttc acttttttaa aagtaatcaa aacagaaggt gttaatcaaa cagaagatag
     5701 accaatgttg ccatttgatg gtacaaatta aacattttta tgaaaaaaaa attacttatt
     5761 gttggtgctg gaagtgttgg taagttcgta gcttataata ttgataattt tactcaagaa
     5821 tttgaaataa tcggtttttt ggatgatgat atttcaaaac agaataccga aatctctgga
     5881 tattttgtaa ttggttcggt tgaaaaactt tcagagtttt ctggaaaaaa tgttgccata
     5941 gtttggggta ttgcttttcc agaaattaaa aaaagattgc tcaacgaata caaacattta
     6001 gactttgaat atcctaattt tatttccaaa aaagcctgga tttctaacca agtaactttt
     6061 ggaaaaggat gcatcgttta tcctgggaat agtattaatt atgaaacaca ggttgaagat
     6121 tttgtagtta taaatatgaa ctgtgccata ggacataatt gtattatagg ttcttttaca
     6181 tcactttcgc ctggtgtaaa tttaggagga aacacaacca taggagatga tgttgaatta
     6241 ggaattggta gtgcaacagt gcaaggaatt tcgattggaa acaacacaaa agttggtggt
     6301 caagctatgg ttgcaaaatc cattgcaagt catcaaatcg ttagaggaat tccagcaaaa
     6361 tcaattcaat aacattgaaa acgaaacgaa tccttgattt tttattttcc tttattatac
     6421 ttctatttat tacttgg
//
LOCUS       DAOOBH010000118         6423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_14189_length_6423_cov_6.322393, whole genome shotgun sequence.
ACCESSION   DAOOBH010000118 DAOOBH010000000
VERSION     DAOOBH010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14189_length_6423_cov_6.322393"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2438)
                     /locus_tag="PK218_10050"
     CDS             complement(<1..2438)
                     /locus_tag="PK218_10050"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPW98892.1"
                     /translation="MSKTTDYISNTMSLREPLKQSLELLDSLIAKADLIGNLKTEKKI
                     ESIKKDCSLFEEFERGFPSICFSIATGIGKTRLMGAFVTYLVKEKGIKNFFILSPNIT
                     IYDKLKDDFGNKGAEKFVFKGLPDISNNAVIITGEDYETNGIKASIDSIQINMFNIGK
                     INSDVKTTTKDGRTLPPRIKRFRETIGDSYYNYLMNLKDLVIIMDESHRYRGERGMET
                     LNELNPLLGIELTATPIDSSTNQRFKNVVFEYGLADALKDGKYIKNPAVVTRADTDFK
                     GMKDDEIEKLKLKDAIQMHRDTKIAIENYVADNNLNPVKPTILVSCKDTTHAKSIFDY
                     VTSDEFYDGEFKEKALQIVSTSKTEEQVEQLLTLEKSHNSIEIVIHVDMLKEGWDVSN
                     LYTIVPLRAANALTLIEQTIGRGLRLPFGGKRTGAEKVDMLSIMMHDKFQEVVDAAKD
                     KNSILYSIGKIQLNKSDLNSKKVTVESKTKDVVELEEEEKKINQIKDKDIRAKAETKH
                     KAKVIINAVIRNKISQKVIQTPNELSTPKVQEKVIAEIKESIKTTYPEMPEKEQKGIA
                     KDIETTYQTAVQEIIKNNILIPNIKVSISENKGIIKDFDLNTKSFSENILQQEFELIR
                     ETIYDHKQDKIKIQAQTLDLPEFSILEFLKKKSEIDYDNNSDVLYKLCGQAVNAIKDS
                     LKKPETLGDTILMYRELIAIKIYEQVKANITIEIVAKSKPEAEPYSLILPWNYSKEKD
                     DEILHFRETVSAKLLSSKVFSGFAKSCHDKYKFDSIPEKRFAEIIENSEDVIKWIRPA
                     SKQFNIWYNNNSDR"
     gene            complement(2442..4109)
                     /locus_tag="PK218_10055"
     CDS             complement(2442..4109)
                     /locus_tag="PK218_10055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPW98893.1"
                     /translation="MNSKYKKLELCWPGKDIEVKPEPRILIEDPEKSYGVAETENMLI
                     HADNLLALKALEQNFSGKIKCVYIDPPYNTGNAFEHYDDGLEHSTWLNLMKPRLELLY
                     KLLSNDGSIWISIDDDESHYLKVICDELFGRKNFVTNVIWEKKFSPQNDAKWLSDSHD
                     HILVYAKNKDIWRPNLLPRTEEMDNRYKNPDNDPRGVWTSSDLTVKTYSESTDYPIKL
                     PSGKVVKPTQSRCWSVSREKFDDLVKENRIWFGENGNNMPRMKRFLNEVQAGAVSKTI
                     WFRTEVGDNQEAKKEVKSFENDNVFTTPKPERLLQRIIQLASNEDDIVLDSFLGSGTT
                     SAVAHKMGRKWLGIELGNHANTHCLPRLKKVCDGTDQGGISKMQKWKGGGGFKFYNLA
                     PSLLNKDKYNQWVINPNYNADLLAAAMARQEGFTYSPDESVVWKQGFATENHFIFTTT
                     NTITKDVVEQILSEMKPDESLRICCTAFIEGCNEISNRIEIKKIPEVLLGKCEFGKED
                     YCTDTNIINMPADPDKPSFIPSYVKPDVKPEPAKIKKDTPTQTKLLF"
     gene            complement(4143..4937)
                     /locus_tag="PK218_10060"
     CDS             complement(4143..4937)
                     /locus_tag="PK218_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98894.1"
                     /translation="MSRIVPFDSNYTTVRKFEKSAFYCLLFSAIAIIYLWLIPKVEPL
                     FSFKISSTTIIDLVKVLSYVSMIGYIGLSSVAKYLFYSAEKVKRGDLIDNSFGTCYSD
                     CNSAGYYNNDDIPFGVKKLAINTYESSFHTEQTLRKMLFKYAMKMLIFSIPFLVSIFS
                     IGGNDVVRLLFEISIPLIMLSQFFIIIVYYTGVKSVNECFKIELTNIGNKAIEVKDNA
                     RFLKPVLDYYNIKSWATTNLDSKIFKNHNDQISELWQKRKEKLKLV"
     gene            complement(4930..5310)
                     /locus_tag="PK218_10065"
     CDS             complement(4930..5310)
                     /locus_tag="PK218_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98895.1"
                     /translation="MKSKYPQFKAKHLANNTVTFNGDLLIKPELPIYTVSVEYRGTLS
                     PRVRVIEPKLVENPPHFYKNTECLCLYHSDDFKWDCRKLVAKEIMSWTIAWLYFYEAW
                     LQTGKWLGPESHHTTNTIKEDKDE"
     gene            complement(5348..6322)
                     /locus_tag="PK218_10070"
     CDS             complement(5348..6322)
                     /locus_tag="PK218_10070"
                     /inference="COORDINATES: protein motif:HMM:NF037736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98896.1"
                     /translation="MTLLESINKLVDNVNITDRQEENIKNSISNLETHLKDEESNLYV
                     NECFTNGSWERDTIIRPLNDVDLFAVLDFEEWKDEYGNKPNPQSVLTKIKNYLNKQND
                     YKDKVKQDRPCVTVHLSDKDFDVLPSFEISGGGYYIPKYDLQNWTESYPKMLTKDLDD
                     THRLRDYKLKQVIRVVKYWNRDFNGKTIPSYHVEEIAINIFKMNTFTNFEEGIRKWFE
                     NVEYNMQSIKFKSNDEYESSLKKAKKVKDKLKEANKFYTDKNEVEAKKIWKDIFGKEF
                     PTVDEAEAKNISKSLTEGTLKYSSTAGISTAVGSSLSASKGFYGDEVL"
BASE COUNT         1863 a         1130 c          944 g         2486 t
ORIGIN      
        1 tatctgtcag aattgttatt gtaccaaata ttgaattgct ttgatgctgg tcttatccat
       61 ttaattacat cctctgaatt ttcaattatt tcagcaaaac gtttttcagg gattgaatca
      121 aacttgtatt tatcgtggca tgatttagca aaaccactaa atacctttga agaaagtagt
      181 tttgcactta cagtttctct gaaatgcaat atttcatcat ctttctcttt cgaataattc
      241 caaggcagaa ttaaagaata tggttctgct tcgggctttg atttagcaac aatttcaata
      301 gtgatatttg cttttacttg ctcataaatt ttaattgcta tcaactcacg atacattaaa
      361 attgtgtcac ccaaagtttc gggctttttc aacgaatctt ttattgcatt tactgcttgt
      421 ccgcaaagtt tatacaatac atcggaatta ttgtcatagt caatttctga tttctttttt
      481 aaaaactcca aaatgctgaa ttcaggcaag tcaagtgttt gtgcttgaat ctttattttg
      541 tcctgcttgt ggtcgtaaat tgtttctctg attaattcaa attcttgttg taaaatattt
      601 tctgaaaacg attttgtgtt taaatcaaag tctttaataa ttcctttatt ttctgaaatc
      661 gaaactttta tgtttggaat taaaatgttg ttttttataa tttcttgtac tgccgtttga
      721 taagttgttt caatatcttt ggctattccc ttttgttcct tttcaggcat ttcaggataa
      781 gtggttttaa tgctttcttt tatttcagca ataacttttt cttgcacctt aggagttgat
      841 agttcattag gtgtttgaat tactttctgg gaaattttat ttcttattac tgcattgata
      901 ataacttttg ctttatgttt tgtttcggct tttgccctga tgtccttgtc ttttatttga
      961 tttattttct tttcttcttc ttcaagttca actacatctt tggtttttga ttcaacggtt
     1021 actttctttg agtttaaatc agatttattt agttgaattt taccgattga ataaagaatt
     1081 gaatttttat cttttgcagc atcaacaacc tcttgaaatt tgtcgtgcat cattatagaa
     1141 agcatatcca ctttttcagc gcctgttctt ttaccaccaa aaggtaaacg caaacctctt
     1201 ccaatggttt gttctattaa tgttaaagcg tttgcggctc tcagaggtac aatcgtataa
     1261 aggtttgaaa cgtcccaacc ttctttgagc atatcaacat gtattacaat ttcaatcgaa
     1321 ttatgtgact tttcaagtgt tagaagttgc tctacttgtt cttccgtttt acttgtagaa
     1381 acaatttgca atgctttttc tttaaactca ccatcataaa attcatcaga agttacatag
     1441 tcaaatattg attttgcgtg tgtagtatct ttgcaagaaa ctaatattgt cggttttaca
     1501 gggttcagat tattgtctgc aacataattt tcaatagcta tttttgtatc tcggtgcatc
     1561 tgaatagcat ctttcaattt cagcttttca atttcgtcat ctttcattcc cttaaaatcg
     1621 gtatcagcac gagtaacaac agcaggattt tttatgtatt tgccatcttt taaagcatca
     1681 gccaaaccat attcaaaaac aacattttta aaacgctggt ttgtacttga atcaatgggg
     1741 gttgccgtta attcaatacc aagtaatgga ttaagttcgt ttagtgtttc catacccctt
     1801 tcaccacgat aacgatgcga ttcgtccatt ataattacaa ggtctttcag gttcatcaag
     1861 taattataat aactgtcgcc tattgtttct ctaaagcgtt ttattcttgg gggtaaagtt
     1921 ctgccgtctt ttgttgttgt ttttacatca gaatttattt tgccaatatt gaacatatta
     1981 atttgtatgg aatctattga tgcttttata ccatttgttt cgtagtcttc gccagtaata
     2041 attactgcat tgtttgaaat atctggcagc cctttaaaaa caaacttttc tgctccttta
     2101 tttccaaaat catcttttaa tttgtcgtaa atagtaatat ttggagataa aataaaaaag
     2161 tttttgattc ctttttcttt tactaaatat gttacaaaag cccccattag ccttgtctta
     2221 ccaatacccg tagcaatcga aaaacaaata gatggaaaac cacgttcaaa ctcctcaaat
     2281 aaagaacaat ccttttttat tgattcaatt ttcttttctg tctttaaatt tccaataagg
     2341 tcggcttttg caattagcga atccaataac tcaagtgatt gtttaagagg ctctcttaaa
     2401 ctcattgtat tgcttatata gtcagttgtt ttgctcatat tctaaaacaa taatttcgtt
     2461 tgcgttggtg tgtctttctt aatttttgcc ggttcgggtt ttacatcagg ttttacatac
     2521 gatggtataa aagaaggttt atcagggtca gcaggcatat tgataatatt ggtatctgtg
     2581 caataatcct cttttccaaa ctcgcattta ccgagaagca cttctggaat ttttttgatt
     2641 tcaattctat tacttatttc attgcaacct tcaataaagg cagtacagca aatacgcaaa
     2701 ctttcgtcag gtttcatttc gctgagaatt tgctcgacta catcttttgt aattgtattt
     2761 gtggttgtga aaataaaatg attttcagtt gcaaaacctt gtttccaaac aacgctttcg
     2821 tcaggcgaat atgtaaaacc ttcctgtctt gccattgctg ctgcaagcaa atcagcgttg
     2881 taatttggat ttataaccca ttggttgtat ttgtctttgt tcaatagact tggagctaga
     2941 ttatagaact taaatccacc accacctttc catttctgca tttttgaaat tccgccttgg
     3001 tctgtaccat cacaaacttt ttttaagcga ggtaaacaat gagtatttgc atggttgcct
     3061 aattcaatac ctagccattt ccgacccatt ttatgggcta ctgcggaagt agtaccactc
     3121 ccaagaaaac tgtctaacac aatatcatcc tcgttagaag caagttgaat gatacgctgt
     3181 aatagtcttt ctggttttgg agttgtaaaa acgttatcgt tttcaaaaga tttcacttct
     3241 ttttttgctt cctgattgtc accaacttct gttctgaacc aaatagtttt actaactgcc
     3301 ccagcttgta cttcgtttaa aaatcttttc atacgaggca tgttattacc attttctcca
     3361 aaccaaatgc gattttcttt tactaaatca tcaaattttt ctcttgaaac agaccagcat
     3421 ctagattgtg tcggttttac aactttgcca cttggtagtt ttattggata gtctgtactt
     3481 tcggaataag tttttacagt taaatctgat gaagtccaaa cacctcttgg gtcgttatct
     3541 gggtttttat atctgttgtc catttcttca gtacgtggta ataaatttgg acgccatata
     3601 tccttatttt ttgcataaac aagaatatgg tcatggctat cacttaacca ttttgcatcg
     3661 ttttgaggtg aaaatttctt ttcccaaata acatttgtta caaagttttt tctaccaaaa
     3721 agttcatcac aaatcacctt taaatagtga ctctcatcat catcaataga aatccaaata
     3781 cttccatcat tcgacaataa tttataaagc aactctaaac gtggtttcat taaattaagc
     3841 caagtgctgt gttcaagtcc atcatcgtaa tgctcaaaag cgttaccagt attgtaaggt
     3901 gggtcaatat aaacacattt aattttgccg gaaaagtttt gttccaatgc tttcaatgca
     3961 agaagattat cggcatgtat gagcatgttt tcagtttcgg ctacaccata cgatttttca
     4021 gggtcttcaa tcaatattct tggttcgggc ttaacctcaa tatcctttcc tggccaacaa
     4081 agttcgagtt ttttatattt actgttcatt caaaatatct gtttaaataa aaatgcaaaa
     4141 tactaaacta atttcagttt ctcttttctt ttctgccata gttctgaaat ttggtcgtta
     4201 tgatttttaa agattttgct gtctaagttt gtggttgccc atgattttat attataataa
     4261 tctaaaacag gctttaaaaa tcttgcgtta tctttaactt caatggcttt gtttccgata
     4321 tttgttaatt caattttaaa acactcatta acgcttttaa ctcccgtata gtaaactatt
     4381 ataatgaaaa attgagataa cataatcagc ggaatactta tttcaaaaag caatctaaca
     4441 acatcattac cgccaattga aaaaatcgaa accaaaaagg gaattgaaaa aataagcatt
     4501 ttcattgcat acttaaagag catttttctt agagtctgtt ctgtatgaaa actgctttcg
     4561 taagtgttta ttgctaattt ctttactcca aaaggtatgt cgtcattatt ataatatcct
     4621 gctgaattgc aatcggaata acacgtacca aatgagttat caattaaatc accacgtttt
     4681 actttttcag ccgaataaaa taaatacttt gcaacacttg aaagaccaat atatccaatc
     4741 attgaaacgt aactaagcac ttttaccaaa tcaataattg ttgttgagct aattttaaat
     4801 gagaataatg gttcaacttt aggaattaac cacaagtaaa taattgcaat agcagaaaat
     4861 aatagacaat aaaaagcaga cttttcaaat tttctgacgg ttgtgtaatt gctatcaaat
     4921 ggtactattc tactcatctt tatcttcttt aattgtattt gttgtatgat gcgattcggg
     4981 tccaagccat ttgcctgttt gcaaccatgc ttcgtaaaag tatagccaag caatagtcca
     5041 tgacattatt tcctttgcaa caagttttct gcaatcccat ttaaaatcat cagaatgata
     5101 caaacaaagg cattctgtat ttttgtaaaa atgaggtgga ttttcaacta gtttaggttc
     5161 tattacacga actcttggtg ataacgttcc tctatactct acacttactg tataaatggg
     5221 caattccggc ttaatcaata aatctccatt aaacgtaaca gtattattag ccaaatgctt
     5281 tgctttaaat tgagggtact tcgatttcat cgccccaatt tgtgcagata catttggttt
     5341 atgtttatta aagaacttca tccccataaa aacctttaga tgctgataaa ctgcttccaa
     5401 ctgcggttga aattcctgct gtacttgaat attttaatgt tccttcagtc aatgatttag
     5461 aaatgttttt tgcttccgct tcatctacag ttggaaactc tttaccaaaa atatctttcc
     5521 agattttttt tgcttcaact tcgtttttgt cggtataaaa tttattcgct tcttttaatt
     5581 tatcttttac ctttttagct tttttcaaag aagattcata ttcatcattt gatttgaatt
     5641 taattgattg catattgtat tcaacattct caaaccattt tcttatgcct tcttcaaagt
     5701 ttgtaaaggt gttcatcttg aaaatattta ttgctatttc ttctacatga tatgaaggaa
     5761 ttgtttttcc attaaaatct cgattccagt attttactac cctgattact tgtttcaatt
     5821 tataatctct taatctatga gtatcatcaa ggtcttttgt taacattttc gggtatgatt
     5881 cagtccaatt ttgtaaatcg tattttggaa tatagtaccc acctccagaa atttcaaaac
     5941 taggtaaaac atcaaaatct ttgtcgctta aatgaacagt tacacaaggt ctgtcttgtt
     6001 ttaccttgtc tttgtaatca ttctgtttat ttaagtagtt ttttattttg gttaatacac
     6061 tttgaggatt tggtttattg ccgtattcat ctttccattc ctcaaagtcc aaaacagcaa
     6121 ataaatcaac atcattcaat ggtcggatta ttgtatctct ttcccacgag ccatttgtaa
     6181 agcactcatt aacataaagg ttgctttctt catcctttaa atgtgtttct aaattcgata
     6241 tagaattttt aatgttttct tcttgtctgt ctgtaatgtt cacattatcg acaagtttgt
     6301 taattgattc aagtagtgtc atatcattaa agttttaaga ttcattattt gcagtgagcg
     6361 ggtcggcaaa aaaagcatgt gtgtggctgc gaagggacgg ttgcagggaa gggcagccac
     6421 act
//
LOCUS       DAOOBH010000119         6388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_14278_length_6388_cov_5.451919, whole genome shotgun sequence.
ACCESSION   DAOOBH010000119 DAOOBH010000000
VERSION     DAOOBH010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14278_length_6388_cov_5.451919"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="PK218_10075"
     CDS             <1..1544
                     /locus_tag="PK218_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW98897.1"
                     /translation="FFTQSVVVKMLPFDNKNEFQVVIDLPEGTTLERTAAVTQEIAQY
                     LSTIPEVVDYQNYIGTSAPITFNGLVRHYDLRGGSNMADIQVNLLHKEERDLQSHDIA
                     KEIRSRVQTIGKKYGANVKIIEVPPGPPVLSTLVAEIYGPNYKDQIKVANQVKGILET
                     TTDIVDVDWMTEDNQVEYKLEVDKEKAMLNGIAPQQVVGNLTYLLKEYPISNLYDENS
                     NDNVGIVLSLDDKDKTSLQDIGNIKIKGSRGNVVPVSDLVKIVQDTLQKTIYRKDQKR
                     VVYVTADMAGALESPVYAILGMNEKLQKMKLPKGYKVDELYMEQPTDESDFTVKWDGE
                     WQITLEVFRDLGIAFMVVIVIIYMLIVGWFQNFKTPMVMMMAIPLSLIGIVLGHWLLG
                     AYFTATSFIGMIALAGVMVRNSVLLIDFIEIRLNDGIPLKQAIIDAGAVRTTPILLTT
                     GAVVIGASIILFDPIFQGLAISLVFGAIVSTILTLLVVPIIYYVTERKKWEKKEALTE
                     KEINN"
     gene            1562..1867
                     /locus_tag="PK218_10080"
     CDS             1562..1867
                     /locus_tag="PK218_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98898.1"
                     /translation="MKLLIITAIAEFEKEIKKILKNAKVKTFSFKEVTGYRDSTEDAL
                     ETNWFTTEMNENTSILFYAFVHDESVDSIFKSIEEHNNKQETLSHIHVAVTNIERSN"
     gene            1883..2068
                     /locus_tag="PK218_10085"
     CDS             1883..2068
                     /locus_tag="PK218_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPW98899.1"
                     /translation="MKNRIIRAVAGTFILISLVLSIYVNQNWLWFTAFVGANLLQSSI
                     TKWCLLEDILTKLGVKD"
     gene            2180..4216
                     /locus_tag="PK218_10090"
     CDS             2180..4216
                     /locus_tag="PK218_10090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HPW98900.1"
                     /translation="MTKFNFLFVLLLVITITSCSKKTTTQEDPIITHRDSTVNPADDF
                     FMYANGNWFKQNPIPASERYNGIFRTIGDTINAQIKQICEKSAKMTDAPKGSNEQKIG
                     DFYTAGMDTATIEKLGISPLTEEINKIKAVKDIPTLLASIAHLHSIGANPAFSIYVAQ
                     DDKISDKYAVFLSQGGLTLGNRDYYFNTDKETENIRNEYVKHIQAMAELAGYSKEIAQ
                     QNAAIIMKMETEMAQNSRKLEALRDPIKNYNKMSLEAFSATTPNIDWKEITTSIGLKN
                     VDSLIVGQPEFYKALNQMVKSYSIENWKTYLEWQLLDGYAGYLNSAFEKQNFYFFSTV
                     MSGVKEQKPRWKRIVEQTNSALGELIGQIYVKEYLPKGSKEKLLEIGNNIRDVYAERI
                     KKLDWMSEETKKKALFKLSKIVMKVGYPDKWKDLSGISIDRKSYIDNIMAVNKWAVND
                     MVSKYGKPVDRTEWDMQPQTYNAYYNPSNNEICVPACNILVPGFEGRMPDDAILYSII
                     GGSTFGHEITHGFDDQGSQYDEKGNLNNWWTAEDLKKFQAKTKLIVDQYNEFEALPGK
                     FVNGDATQGENIADLGGIVMGYEAFKKTAQYKNKEIISGLSPEKRFFLGYAYAWMVNY
                     KTESLAQQLLTDVHSPAKYRINGPLQNMPEFFEAFGIKPGSKMRRADDKIIVIW"
     gene            complement(4241..5023)
                     /locus_tag="PK218_10095"
     CDS             complement(4241..5023)
                     /locus_tag="PK218_10095"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPW98901.1"
                     /translation="MNTNKILTIVFLGSLLVMSCNNKKLTEVVEVPLPSVDDKITIGL
                     PEDVRVEDGAFSLTKLPDNYEAFAPSIGVLTMENHYSKHYLTYTNNLNTLIAANQALV
                     DLSIEDILKKLDINNVDLRNNAGGYYNHTLFFENLTPKSSGQPKDTLASAIARDFGSF
                     ELFKTQFTDAAEDQFGSGWAWLIVDNTGKLVITSTPNQDNPLMPKQVVSGTPILNLDV
                     WEHAYYLDYQYKRRRYIDAFYKIINWKKVGERYEEALKNKKP"
     gene            complement(5046..5312)
                     /locus_tag="PK218_10100"
     CDS             complement(5046..5312)
                     /locus_tag="PK218_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HPW98902.1"
                     /translation="MSEKDLDTRFNEAVAIASKMSQASLPQDVQLRLYAYYKQATIGT
                     LSTNSSSGLDLRNAFKTNAWMQISHLSVDQAKELYIEMIHSLEL"
     gene            complement(5322..5972)
                     /locus_tag="PK218_10105"
     CDS             complement(5322..5972)
                     /locus_tag="PK218_10105"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009419689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPW98903.1"
                     /translation="MFHKEGYKIIFITLVSFIAGILLLDKFVELAWLKMTLQLVLLFF
                     LLIVLQFFRNPKRVVDHSIHNLIAPVDGKVVVIEEVFEPEYFKDKRLQVSIFMSPINV
                     HVTRYAMSGKINYSKYHPGKYLVAWHPKASTENERTSVVIENEVFGEVLYRQIAGALA
                     KRIVNYAIEGTEVVQGEDAGFIKFGSRVDLYLPLGTEVNVKLNQKAVGGKTIIAVK"
     gene            complement(5962..>6388)
                     /locus_tag="PK218_10110"
     CDS             complement(5962..>6388)
                     /locus_tag="PK218_10110"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPW98904.1"
                     /translation="PMLIICIFILIWTNDTFAYLIGKNFGKNKLFPSVSPKKTVEGFV
                     GGLFFSIIAGVLISKFYINPKPSYLNTSILIWSISAIIISIFSTLGDLIESKLKRIVG
                     VKDSGVIMPGHGGILDRLDSIIFVIPFINLFYIILQYVS"
BASE COUNT         2305 a          999 c         1096 g         1988 t
ORIGIN      
        1 tgtttttcac ccaatcggtg gtggttaaaa tgcttccatt tgataataaa aatgaattcc
       61 aagtagtgat tgatttaccg gaaggaacta ctttagaacg aacggctgcg gttactcaag
      121 aaattgctca atatctttct actattccag aagtggttga ttaccaaaac tatattggaa
      181 cttcggcacc aattactttt aatggtttag ttcgtcatta tgatttgcgt ggcggaagta
      241 atatggcaga tattcaagtg aatttattgc acaaagaaga acgagattta caaagtcatg
      301 atattgcaaa agaaattaga agtagagtac aaactattgg gaaaaaatat ggtgccaatg
      361 tgaaaattat tgaagttccg cctggaccac cagttttatc aacattagtt gcggaaattt
      421 atggcccaaa ttacaaagac caaataaaag tagccaatca agtaaaagga attcttgaaa
      481 ctacaaccga tattgttgat gtggattgga tgacggaaga taatcaagtg gaatacaaat
      541 tggaagttga taaagaaaaa gcaatgctta atggaattgc accacaacaa gtcgttggaa
      601 atttgacgta tttattgaaa gaatatccaa tttcaaattt gtatgatgaa aattcgaatg
      661 ataatgttgg aatagtactt tcattagatg acaaagacaa aacatcattg caagacattg
      721 gaaatataaa aataaaagga agtcgaggaa atgtggtgcc agtttcagat ttagttaaaa
      781 tagtgcaaga cacattgcag aaaaccatct atagaaaaga ccaaaaacgt gtggtttatg
      841 ttacagctga tatggctgga gcattagaaa gtccggtgta tgctattttg ggaatgaatg
      901 agaaattgca aaaaatgaaa ttaccaaaag gttataaagt tgatgaattg tacatggaac
      961 aacccacaga tgaaagtgat ttcactgtaa aatgggatgg agaatggcaa attacgctgg
     1021 aagttttccg cgatttggga attgcgttta tggtggttat tgtaatcatt tatatgttga
     1081 ttgtaggttg gtttcaaaac ttcaaaacgc caatggtgat gatgatggcg attccacttt
     1141 cattaatcgg aattgtttta gggcattggt tgttaggcgc ttattttaca gcaacatcat
     1201 ttattggaat gattgcatta gctggtgtta tggtgaggaa ttcagttttg ctcatcgatt
     1261 ttatagaaat aaggttgaat gatggtattc cattaaaaca agccataatt gatgctggag
     1321 ctgtgagaac tacaccaatt ttgttaacca ctggtgctgt tgttattgga gcatcgatta
     1381 tactatttga cccaatattt caaggattag ccatatcatt agtttttggt gcaatagttt
     1441 ctaccatatt aactttgtta gttgtaccaa ttatttacta cgtaactgaa cgtaaaaaat
     1501 gggaaaagaa agaagcgtta acagaaaagg aaattaataa ttaagtaaaa aataatttaa
     1561 aatgaaacta ttaattatca ctgctatagc cgaatttgaa aaagaaataa aaaaaatatt
     1621 aaaaaatgct aaggttaaaa cattctcttt taaagaagtt acaggctatc gcgatagtac
     1681 agaagatgcg cttgaaacca attggttcac tactgaaatg aatgaaaata catcaatttt
     1741 attttatgct tttgttcacg atgaaagtgt ggattcaatt ttcaaatcta ttgaagaaca
     1801 taataataag caagaaacgt tatcgcatat acatgtggca gtaaccaata tagaaagatc
     1861 aaattaactc aaattaaaac gtatgaaaaa tagaattatt agagcagttg caggaacatt
     1921 catccttata agtttagtac tttccattta tgttaatcaa aattggcttt ggtttacagc
     1981 ctttgttgga gcaaatttgc tgcaatcatc tataacaaaa tggtgcttat tagaagatat
     2041 tctcactaag ctaggcgtaa aagactaaaa tttaaagctt cattgaaaaa tgaggctttt
     2101 tttaggactt atttttttac ttttggtaac tattagtaac aaatactact aataaattac
     2161 atttaacaaa actgtaacta tgacgaaatt caacttccta tttgttctac ttttagtaat
     2221 aacaattaca agctgttcca aaaaaacaac cactcaagaa gacccaatca ttacgcatcg
     2281 cgattcaaca gttaatcctg ctgatgattt ttttatgtat gccaatggaa attggtttaa
     2341 acaaaaccct atcccagcta gtgaaagata caatggaatt ttcagaacta ttggcgatac
     2401 tattaatgct caaataaaac aaatttgcga aaaatcagcc aaaatgacag atgctccaaa
     2461 aggtagtaac gaacaaaaaa ttggtgattt ctataccgca ggtatggata ctgcaaccat
     2521 tgaaaaactt gggatttctc cattaaccga agaaattaac aaaatcaaag cagtaaaaga
     2581 tattccaact ttattggcat cgatcgctca tttacattct attggagcaa atcctgcatt
     2641 ttcaatttat gtagctcaag atgataaaat cagtgataaa tatgctgtat ttttaagtca
     2701 aggcggacta actttgggca atcgcgatta ctattttaat accgataaag aaacggaaaa
     2761 cattagaaat gaatatgtaa aacatattca agctatggca gaacttgcgg gctattctaa
     2821 agaaatagca caacaaaatg cggcaattat catgaaaatg gaaactgaaa tggctcaaaa
     2881 ttcaagaaaa ttagaagctc ttcgcgatcc tattaaaaat tataataaga tgagtttaga
     2941 agctttttca gctactactc caaatataga ttggaaagaa attacaactt ccattggttt
     3001 aaaaaatgta gatagtttga ttgttggtca accagaattt tataaagcat taaatcaaat
     3061 ggtcaaaagt tattcaatag aaaactggaa aacttattta gaatggcaat tacttgatgg
     3121 ctatgccggg tatttgaata gtgcttttga aaaacaaaat ttctatttct tttcaaccgt
     3181 aatgagcggc gtgaaagaac aaaaaccacg ttggaaaaga attgttgaac aaaccaatag
     3241 tgctttgggt gagttaatcg gtcaaattta tgtgaaagaa tatttgccaa aaggctctaa
     3301 agaaaaacta ttagaaattg gaaataacat tcgtgatgtt tatgccgaac gcatcaagaa
     3361 attagattgg atgagtgaag aaactaagaa aaaagcactt ttcaaactaa gtaaaattgt
     3421 gatgaaagtg ggttatccag ataaatggaa agatttaagc ggaatatcta ttgatagaaa
     3481 atcatacatt gataatataa tggctgtcaa taaatgggct gttaacgata tggtttcaaa
     3541 atacggcaaa cctgtagata gaaccgaatg ggatatgcaa ccacaaacct ataatgctta
     3601 ttacaaccca agcaacaacg aaatttgtgt gccagcatgt aacattttag tgcctggttt
     3661 tgaaggcaga atgcctgatg atgctatcct ttacagtatc attggaggat caacatttgg
     3721 acatgaaatc acgcatggtt ttgacgatca aggaagtcaa tatgatgaaa aaggaaacct
     3781 aaacaattgg tggacagccg aagatttgaa aaaattccaa gccaaaacaa aactgattgt
     3841 tgatcaatac aacgaatttg aagcattgcc tggtaaattt gtaaatggtg atgccactca
     3901 aggtgaaaat attgcagacc ttggtggaat tgtcatggga tatgaagcct tcaaaaaaac
     3961 agcacagtat aaaaataaag aaattattag tggtttatca cccgaaaagc gtttcttttt
     4021 aggctatgct tatgcttgga tggttaacta caaaacagaa tctttagcac agcaattatt
     4081 gaccgatgta cactcgccag caaagtatag aataaatgga ccgttgcaaa acatgcctga
     4141 attcttcgaa gcatttggta taaaaccagg aagcaagatg cgaagagctg atgataagat
     4201 tattgttatt tggtaataaa acaagaaacc ctaacaaaag ttagggtttc ttgtttttta
     4261 gagcttcttc atagcgttca ccaacttttt tccagttaat gattttgtaa aaagcatcaa
     4321 tatagcgtcg tcgcttgtat tggtaatcta aataataggc atgttcccaa acatcaagat
     4381 ttaaaattgg tgttcccgaa accacttgtt ttggcatcaa cggattgtct tgatttggtg
     4441 tacttgtaat gactaatttt ccagtattat caacaattaa ccaagcccaa ccggaaccaa
     4501 attgatcctc tgcagcatct gtaaattgag ttttaaataa ctcaaaactt ccaaaatccc
     4561 tagcaattgc tgatgctaaa gtatctttag gttgcccgct agattttggt gtgaggtttt
     4621 caaaaaacaa ggtgtgatta taataaccac cagcattatt ccttaaatcg acattgttta
     4681 tatcaagttt ttttaaaata tcctcaatac ttaaatcaac caaagcttga ttggcagcaa
     4741 ttagtgtgtt caaattattg gtataggtca aataatgttt tgaataatga ttctccatgg
     4801 tcaatacacc aatacttggt gcaaatgctt catagttatc aggaagcttt gttaaggaaa
     4861 aagcaccatc ttcaacacga acatcttcgg gcaaaccgat agtgatttta tcatcaacag
     4921 atggcaatgg cacttccacc acttcggtta gctttttatt attacaactc ataacaagta
     4981 agcttccaag aaaaacaatg gtaagtattt tatttgtatt catttttgct aaaattattt
     5041 gaaatttata gctctaaaga atgtatcatt tcaatataca attctttggc ttggtcaaca
     5101 gaaagatgac ttatttgcat ccatgcgtta gttttgaatg catttcttaa atctaaacct
     5161 gaactcgaat tggtactcaa ggtaccaatt gtagcttgtt tgtaataggc atataatcgc
     5221 aattgtacat cttgaggcaa agatgcttga gacatttttg aagctattgc tacggcttca
     5281 ttaaatcgtg tatctaaatc tttttcagac atagaaaatt attattttac agcgatgata
     5341 gtttttccac caaccgcttt ctgattgagt tttacgttaa cttccgttcc taaaggtaaa
     5401 tataaatcaa ctcttgagcc aaatttgata aatccagcgt cttctccttg tactacttcg
     5461 gttccttcta tcgcatagtt tacaatacgt tttgccaaag cacccgctat ttgtcggtat
     5521 agaacttctc caaatacttc gttttcgata actacagaag ttcgttcgtt ttcggtactt
     5581 gcttttggat gccaagctac caagtatttc ccaggatggt atttgctata attgattttc
     5641 ccgctcattg cataacgcgt tacgtgcaca tttattggcg acataaatat cgatacttgc
     5701 agtcttttat ctttgaaata ttctggttca aaaacctctt caatcaccac tacttttcca
     5761 tcaactggag cgataagatt gtgaatagaa tgatccacca ccctttttgg attgcggaaa
     5821 aattgcaata caattagcaa gaaaaaaagc aacaccaatt gcaaagtcat ttttaaccaa
     5881 gctaactcta cgaatttatc caataaaagg atacccgcaa taaagctaac taatgtaata
     5941 aaaataatct tgtaaccttc tttatgaaac atattgtaaa attatataaa acaaattaat
     6001 aaatggaatt acaaatataa tactatctaa gcgatctaaa atacctccat gaccaggcat
     6061 aattactccg ctatccttta cacctacaat tcgctttaat ttagattcaa tcaaatctcc
     6121 taaagtactg aaaatactta tgataatagc acttattgac caaattaata ttgaagtatt
     6181 gagataacta ggttttggat taatataaaa tttagaaatt aaaaccccag caattattga
     6241 aaaaaacaat cctccaacaa aaccttctac tgtttttttt ggtgaaacag aaggaaataa
     6301 tttattcttt ccaaaattct ttcctattaa gtatgcaaaa gtatcatttg tccaaataag
     6361 aataaagata catattatta acattggt
//
LOCUS       DAOOBH010000120         6244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_14668_length_6244_cov_5.244466, whole genome shotgun sequence.
ACCESSION   DAOOBH010000120 DAOOBH010000000
VERSION     DAOOBH010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14668_length_6244_cov_5.244466"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1024
                     /locus_tag="PK218_10115"
     CDS             311..1024
                     /locus_tag="PK218_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW98905.1"
                     /translation="MGRAFEFRKGRKMKRWAAMAKTFTRIGKDIVMAVKEGGPNPEAN
                     SRLRAVIQNAKSANMPKENVERAIKKATDKDTANYKEVLFEGYAPHGIAILIETATDN
                     NNRTVANIRSYFNKCNGTMGTQGSVEFMFDHTCNFRIPKGEIDIEEMELEFIDFGAEE
                     IFVDEDDILIYAPFESFGAIQKELENRGIEILSSGFERIPQITKELKSEQEIADVEKL
                     LEKIEEDDDVMNVYHTMKE"
     gene            complement(1112..1822)
                     /gene="pepE"
                     /locus_tag="PK218_10120"
     CDS             complement(1112..1822)
                     /gene="pepE"
                     /locus_tag="PK218_10120"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HPW98906.1"
                     /translation="MKKLIIASTSTLHGGNYLEYILPELKKHFSNVSTLLFIPYAQPS
                     GISYDDYTNIAKRAFATIDVKVNGIHEFENPTEAIENAEGIFTGGGNTFLLVTKLFEN
                     KVIDTLKKVVENGTPYLGSSAGSNITGLTMQTTNDMPIIYPPSFETLGFLPFNLNPHY
                     LDPDLQSKHMGETRETRIKEYHTLNSSPVLGLREGSWLEVTGKKILLKGNLTARWFRQ
                     NEKPIELNPETDLSLLEH"
     gene            1934..2692
                     /locus_tag="PK218_10125"
     CDS             1934..2692
                     /locus_tag="PK218_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98907.1"
                     /translation="MKFKLLCLTLLYSCFALAQNDELVFLKGKIVSEIKELNEILVLN
                     SRSESTTTTDFKGDFSLFAKVGDTLKFSGLQVVSKNIVLTKNDISKTLFAVTLQPKVF
                     ELNEVKVNEYPEINAVSLGILPKAPKKYTPAERKLSAAGVFKWYSPLLIPFGGMSVDG
                     LLNSISGRTAMLKKELEIERKEMLKKKLENYFEEDYFTNTLKIPAENIDGFLYYAVDD
                     EKLVKLASENNKSLLQFYLGEIATKYIKITVPKE"
     gene            2703..3437
                     /locus_tag="PK218_10130"
     CDS             2703..3437
                     /locus_tag="PK218_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98908.1"
                     /translation="MKKKYYLIVFCLTQMCFSQSIMRKNLKGQVVNDSLKVDNVVIFN
                     INNQKGTTVYNDGSFEIEVRENDTLFFSSLSFLPEKILITQREIDKGMLTVPLEAFMN
                     KLQEVVVRTKIKPNIDTNRNIIDTQYTNDAKSSLKNPYIYTGEIQNGVDFARIFKDIG
                     RIFKKKKKEKQAQEILFTDLVMKKIDYDFYKTTLKLKDEEIRLFLYYCEKGEHDFEQF
                     NTKTNFEIMNFLIDKNKEYQEFKVKK"
     gene            3564..3887
                     /locus_tag="PK218_10135"
     CDS             3564..3887
                     /locus_tag="PK218_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPW98909.1"
                     /translation="MFGIGGGELFFILFIALMLFGSDKIPTIARTLGKAMAQLKNATN
                     EIKSEIQKSAEENGLDKSMKELTTSFTKEIDEVKAGIDPNSITKFQKEIEDIEEMTGP
                     IKRQK"
     gene            3887..4444
                     /locus_tag="PK218_10140"
     CDS             3887..4444
                     /locus_tag="PK218_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPW98910.1"
                     /translation="MLEKLIDLDHKIFIYLNGLGSETFDSLWLLITKQIYWTPFFLFI
                     FYLLQKKLGWKKFGYYILFTAILILICDQTANLFKNSFQRLRPCNVEELKGMIRIVKT
                     SNSFSFFSGHAVNSMATTVFGFMILKQFYKHSYLLFLFPFIFAYSRIYLGLHFPSDIL
                     TGYFFGATFGFLFFKLYQKYVLNKA"
     gene            complement(4454..5095)
                     /locus_tag="PK218_10145"
     CDS             complement(4454..5095)
                     /locus_tag="PK218_10145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPW98911.1"
                     /translation="MHFLSDELDHYVTVHSQDEPELLAQLNKETFQKILQPRMLSGHF
                     QGRVLSLLSKIINPKTVLEIGTYTGYATLCMAEGLDENGTIDTIDIEEELVDFQRKYF
                     DKSSWGNQITQHLGDALKIIPNLNKKYDLVFIDADKENYINYFHLIVPMMNKGGIILS
                     DNVLWSGKVIEEVKANDVTTKILLEYNKLLKEDSRVETVLLPIRDGLTVSRKL"
     gene            5156..6196
                     /gene="rlmN"
                     /locus_tag="PK218_10150"
     CDS             5156..6196
                     /gene="rlmN"
                     /locus_tag="PK218_10150"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPW98912.1"
                     /translation="MEIEKKDIRSLSKEQLRDFFVANGDKAFRGNQVYEWLWSKASHS
                     FEDMTNVSKETRTMLENNFVINHIKVDTIQRSDDGTVKNAVRLHDDLIVESVLIPTKT
                     RTTACVSSQVGCSLDCNFCATARLKRMRNLNPDEIYDQVVAIDRESRLYFNRPLSNIV
                     FMGMGEPLMNYPNVMKAIDKITSNEGLGMSAKRITVSTSGIPKMIRKMADDDVKVKLA
                     VSLHSGIDDVRSKIMPFSQNFPLKDLRESLEYWYQKTKSKITYEYVVWKGINDSKEAV
                     DALVKFCKYVPCKVNLIEYNPIDDGEFQQASDDAINLYIKELEKNNIVAKVRRSRGKD
                     IDAACGQLANKS"
BASE COUNT         2316 a          853 c         1016 g         2059 t
ORIGIN      
        1 ccaatagctg tatcaaaact ttgagaaagt aaaacctgag attgagagtt tattgaatta
       61 ataatcaaaa taaaaaagta aaattttttc atataaattt tgttaagttt tttgtaaaaa
      121 tatattattt tttaatccaa aataattata cgtcaaatat tttagctaat aattttaaat
      181 ttaatcttta agtaatgtcg tatattaata aataagtttc caattcaaaa tttaaaaaat
      241 tacattggtt gaaaaaaaca atttgaaata catataataa ttataatttt gcaaaaatta
      301 aaaacaagaa atgggaagag cattcgaatt tagaaaagga agaaaaatga agcgttgggc
      361 agcaatggcc aagactttta cacgtatagg taaagacata gtaatggctg tgaaagaagg
      421 aggtccaaat cctgaagcca attcgcgttt aagagctgtt attcaaaatg cgaaatcggc
      481 aaacatgcct aaagaaaacg tagaacgtgc catcaaaaaa gcgactgata aagatactgc
      541 caactataaa gaagtattat ttgaaggtta tgctccacac ggtatcgcta ttttaattga
      601 aaccgcaacg gataacaata acagaaccgt tgcaaatatt agaagttatt ttaataaatg
      661 taatggaacg atgggaacgc aaggttcagt tgaatttatg tttgaccata cgtgtaattt
      721 cagaattcca aaaggggaaa ttgatattga agaaatggaa ttagaattca ttgattttgg
      781 tgcagaagaa atttttgttg atgaagatga catattaatt tatgcaccat ttgaaagttt
      841 tggtgctatt caaaaagaat tagaaaaccg tggaattgaa atattatctt ctggatttga
      901 acgtattcca caaattacca aagaattaaa atccgaacaa gaaattgcag atgtcgaaaa
      961 actgttagaa aaaattgaag aagatgatga tgtaatgaat gtctatcata ctatgaaaga
     1021 ataataaata atcaaaaaat gctaaaaaac gcctagaaaa gatttctagg cgtttttaat
     1081 tagtgatgat taaaaactaa acttaataca attaatgttc tagcaaagat aaatccgttt
     1141 ctggattaag ctcaattggt ttttcgtttt gtctaaacca tcgagcagtt aagtttcctt
     1201 ttaagagtat ctttttacca gttacttcga gccaactccc ttctcgcaaa cctagaaccg
     1261 gacttgaatt tagcgtatga tattctttaa ttcttgtttc tcttgtttct cccatgtgtt
     1321 tgctttgcaa atctggatcc agataatgag gattaagatt gaaaggtaaa aaccctaaag
     1381 tttcaaagct aggcggataa ataataggca tatcattagt ggtttgcatg gttaaccctg
     1441 taatattact acctgcactt gaaccaaggt agggcgtacc gttttcaaca acttttttta
     1501 atgtatcaat aaccttgttt tcaaaaagtt ttgtaaccaa aagaaaagtg tttccaccac
     1561 cagtaaaaat cccttccgca ttttcaatag cttcagtagg gttttcaaat tcatgaattc
     1621 catttacttt aacatcaatt gttgcaaatg ctcttttagc aatattggta taatcatcat
     1681 aagaaattcc acttggttgc gcatagggaa taaaaagtag tgttgaaaca tttgaaaaat
     1741 gtttttttag ttcaggcaaa atatattcta aataatttcc gccatgaagt gtagaagtac
     1801 ttgcaattat taatttcttc ataaaaaaga tgtatttctc caaaagtaac attttccttt
     1861 tttatttaac aaaaaattac aagcacaatt gatgattttt aaacatcttt gaaaaagtat
     1921 taatcaaaca attatgaagt ttaaattact ttgcttaact ttattgtata gttgttttgc
     1981 attggctcaa aatgatgaat tggttttttt gaaaggaaaa atagtttctg aaattaaaga
     2041 attaaatgaa attttagtcc tcaattcacg atcagaatcg acaacaacaa ctgattttaa
     2101 aggtgatttt tctctttttg caaaagtagg cgatacatta aagttttcgg ggttacaggt
     2161 agtttcaaaa aatattgtat tgactaaaaa tgatatttca aaaacacttt ttgcagtaac
     2221 attacaacca aaagtatttg aattaaacga agttaaagtc aatgaatatc cagaaataaa
     2281 tgcagtttct ttaggtattc ttccaaaagc tccaaaaaaa tatactccag cagaacgcaa
     2341 acttagtgcc gcaggagtat ttaaatggta cagcccgttg ttaatacctt ttggaggaat
     2401 gagcgtggat ggcttgttga acagtatttc aggaagaact gcgatgctta aaaaagaact
     2461 cgaaattgaa aggaaagaga tgttaaaaaa gaaacttgaa aattattttg aggaagatta
     2521 ctttaccaat acactaaaaa ttccagcaga aaatattgat ggatttcttt attatgctgt
     2581 tgatgatgaa aaattagtga agcttgcatc cgaaaataac aaaagcttac tacagtttta
     2641 tttaggagaa atagctacaa aatatataaa aataacagtt ccaaaagaat aaataacatg
     2701 tcatgaaaaa aaaatattat ttaattgttt tttgtctaac gcaaatgtgt ttttcacaat
     2761 caataatgcg caaaaacctt aaaggtcaag tagtaaatga ttctttaaaa gtggataatg
     2821 ttgtgatttt taacattaat aatcagaaag gaacgactgt ttataatgat gggtcgtttg
     2881 aaattgaagt tagagaaaac gatactttgt ttttttcaag tttatctttt ttacctgaaa
     2941 aaatactgat aacacaacgc gaaattgata aagggatgtt gactgttcct ttagaagctt
     3001 ttatgaacaa acttcaagaa gttgtggtta gaactaaaat aaaaccaaac attgacacca
     3061 atagaaatat cattgatacc caatatacta acgatgctaa atcatcattg aaaaatcctt
     3121 atatctacac tggagaaatt caaaatggag ttgattttgc tagaattttc aaggacattg
     3181 gacgaatatt taagaagaaa aagaaagaaa aacaagctca agaaatattg tttaccgatt
     3241 tggtgatgaa aaaaattgat tatgattttt ataaaacaac cttaaaactg aaagatgaag
     3301 aaatacggtt atttttatat tattgtgaaa aaggcgaaca tgattttgaa caatttaaca
     3361 ccaaaactaa ttttgaaatc atgaatttct taatcgataa aaataaagaa tatcaagaat
     3421 ttaaagttaa gaaataaact ttttttatat ataagaatta taccaaaaat tgacaaatca
     3481 aaaaaatagg ttaagctttt ggttaaagtc ttatatgcta aaaaatcttt gtacatttgc
     3541 acttcaaaat taatttttaa tatatgttcg gaattggtgg tggcgaattg ttttttatac
     3601 tttttattgc tttgatgctt tttggttcgg ataaaattcc aactattgct agaactttag
     3661 gaaaagctat ggcacaatta aaaaatgcaa ccaacgaaat taaaagtgaa attcaaaaaa
     3721 gtgccgaaga aaatggtctt gataagtcta tgaaagaatt gacaacgtct ttcacaaaag
     3781 aaattgatga agtaaaagca ggaattgacc caaacagcat cacaaaattc caaaaagaaa
     3841 ttgaagacat tgaagaaatg acaggaccga ttaaacgtca aaaataatgt tggagaaact
     3901 aatagattta gaccataaaa tttttattta tctaaatgga ttaggttcgg aaacctttga
     3961 tagtttatgg cttttgatta ccaaacaaat ttactggaca cctttttttc ttttcatatt
     4021 ttatttacta caaaagaaat taggctggaa aaaatttggg tattatattc ttttcactgc
     4081 aattttaatt ttaatttgcg accaaaccgc caatcttttt aaaaactctt ttcagcgact
     4141 tcgcccgtgt aatgttgagg aattaaaagg aatgattaga attgtaaaaa ccagtaattc
     4201 gttcagtttt ttttctggtc atgctgtcaa ttctatggca actacagttt ttggatttat
     4261 gattttaaaa caattttata aacacagtta tttactattt ctttttccat ttatatttgc
     4321 ttacagtaga atttacttag ggttgcactt tccttctgat attttaacag gttatttttt
     4381 tggtgctact tttggttttc ttttttttaa attatatcaa aaatatgtct taaataaagc
     4441 gtagcacgaa attttacaac tttctcgaaa ccgtcaaacc atcgcgaatt ggtagtaaaa
     4501 cagtttctac tcttgaatct tcttttaaaa gcttgttgta ttctaaaaga atttttgtgg
     4561 taacgtcatt tgctttcact tcttctatta cttttccgct ccaaagcaca ttatccgaaa
     4621 gaataatacc gcctttattc atcattggaa ctatcagatg aaaataattg atataatttt
     4681 ctttatcggc atcgatgaaa accaaatcgt attttttatt tagattggga atgattttca
     4741 aagcgtcacc aagatgctga gtaatctgat ttccccaaga acttttgtca aaatattttc
     4801 gttgaaaatc gaccaattct tcttcaatat caatagtgtc aatagttcca ttttcgtcca
     4861 aaccttccgc catacaaagc gttgcataac ccgtataagt gcctatttct aaaacagttt
     4921 ttggattaat aatttttgaa agtaggctca aaactctacc ttgaaaatga ccgctcaaca
     4981 ttcgaggctg taatattttt tgaaaggttt ctttgtttaa ttgtgctaac aactctggtt
     5041 cgtcttgcga atgaacagta acataatggt ctaattcgtc ggaaagaaag tgcatgattt
     5101 tattttttac aaaaatacaa attaagaagt aatcaaaata atctaaattt gcaccatgga
     5161 aattgagaaa aaagacatac gaagtttatc gaaagaacag ttgcgagatt tttttgttgc
     5221 caatggtgat aaagcatttc gtggcaatca ggtttatgaa tggctttgga gcaaagcgtc
     5281 acatagtttt gaagacatga ccaatgtttc caaagaaaca agaaccatgt tagaaaataa
     5341 tttcgttata aatcatatca aagttgatac tattcaacgt agtgatgatg gaaccgtaaa
     5401 aaatgccgtt cgcttgcacg atgatttgat tgttgaaagt gttttaattc caacgaaaac
     5461 aagaactaca gcttgcgttt ccagtcaagt aggttgtagt ttggattgta atttttgtgc
     5521 tactgctcgg ttaaaacgaa tgcgaaattt aaatcctgat gaaatctacg atcaagtagt
     5581 tgccatagat agagaaagtc gtttgtattt caatcgtccg ctttccaaca ttgtttttat
     5641 gggaatgggc gaacctttga tgaattatcc taatgtgatg aaagcgattg acaaaatcac
     5701 atctaacgaa ggtttgggaa tgtcggcaaa acgaattaca gtttccactt cgggaatccc
     5761 aaaaatgatt agaaaaatgg cagatgatga tgtaaaagtc aaattagcag tttctctaca
     5821 ctctggaatt gatgacgtta gatccaagat aatgcctttt tcacaaaatt ttccattaaa
     5881 agatttacga gaaagtttgg aatattggta ccaaaaaact aaaagcaaaa ttacctacga
     5941 atatgtggtt tggaaaggaa taaatgatag taaagaagcg gttgatgctt tggtgaaatt
     6001 ttgtaaatat gttccttgca aagtaaattt aatcgaatat aatccaattg atgatggcga
     6061 atttcaacaa gcttctgatg atgcaatcaa tttatatata aaagaattgg agaaaaacaa
     6121 tatagtggca aaagtgcgac gcagtagagg aaaagatatt gatgctgctt gtggtcaatt
     6181 ggcaaataaa tcataaaaaa aacgtttcaa atttttgaaa cgttttctag taagtgtgta
     6241 aatt
//
LOCUS       DAOOBH010000121         6113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_15036_length_6113_cov_6.069330, whole genome shotgun sequence.
ACCESSION   DAOOBH010000121 DAOOBH010000000
VERSION     DAOOBH010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15036_length_6113_cov_6.069330"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PK218_10155"
     CDS             <1..498
                     /locus_tag="PK218_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPW98913.1"
                     /translation="PADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLDSTSR
                     ILTAQILGDEHYDCAQRVKEILQKYKQLQDIIAILGMEELSEEDKLSVSRARRVQRFL
                     SQPFHVAEQFTGIPGVLVDIKDTIKGFNMIIDGELDHLPEAAFNLKGTIEDVIEAGQK
                     MLAEA"
     gene            591..872
                     /locus_tag="PK218_10160"
     CDS             591..872
                     /locus_tag="PK218_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPW98914.1"
                     /translation="MILEIVSPEASLFKGEVTSVYLPGVDGSFQILNNHAPIVSILKQ
                     GTVKITANSFTFEKEVAEKFSKVNDQNYTLEINSGTIEMKDNKVIVLAD"
     gene            935..1462
                     /locus_tag="PK218_10165"
     CDS             935..1462
                     /locus_tag="PK218_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98915.1"
                     /translation="MKNNNEFELIVAQGENPWWRLLLSVAFFTLMVWVVSENIIMFYN
                     YGFHEDTFRVFSKSLFTIGYSLTGGIIFAVTKTILIDVDKDLLISRFNVGPFSKDVKS
                     TVPQLEYVSIFLDDKENYQVNLWYIGNKHYNMYQFDEKEIAMKFGNHVAIKLNLDLLD
                     ATERGDNKWVEKIKV"
     gene            1459..2613
                     /locus_tag="PK218_10170"
     CDS             1459..2613
                     /locus_tag="PK218_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPW98916.1"
                     /translation="MKLPKALQSKLKLREENNALRYLPLTNTLVDFASNDYLGFAKNE
                     LIFDVSHQLLVNQNLKINGATGSRLLSGNHSLYPLTEDFIAQFHQSEAALIFNSGYDA
                     NVGFFSTVPQRNDMILYDELCHASIRDGIQMSHAKAYKFQHNDFLDLEKLIHKITSNF
                     QLPASIYIVTESVFSMDGDSPNLEELTNLSEKYNCYLVIDEAHSLGVFGSQGEGLVQS
                     VALHEKVFARIMTFGKGLGCHGAAILGSNDLKNYLVNFARSFIYTTGLSPHSVATILM
                     SYRQLIVQEEALQKLKNNIIHFNQEKMRLGLKPLFVYSKSAIHCAIISGNQNVKATSE
                     LLQEKGFDVKPILSPTVSEGQERLRFCLHSYNSHQQITKVLELLSSCVFK"
     gene            2667..3284
                     /gene="bioD"
                     /locus_tag="PK218_10175"
     CDS             2667..3284
                     /gene="bioD"
                     /locus_tag="PK218_10175"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HPW98917.1"
                     /translation="MKLFITGIGTDVGKTIASSIVTQALEADYWKPIQAGDLENSDSH
                     KIQKYISNNKTVIHDNSYKLNTPASPHYAAEIDGITIDVKKIKEPKTANHLVIEGAGG
                     ILVPINNNDSIVNLIQPDYKVIVVSRHYLGSINHTLMTFEVLQSRNIKVAGIIFSGDE
                     NTATEAIILSKTKANFIGRIENEPYFDHNVIQYYADKFRDNLEKL"
     gene            3399..4670
                     /gene="bioA"
                     /locus_tag="PK218_10180"
     CDS             3399..4670
                     /gene="bioA"
                     /locus_tag="PK218_10180"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HPW98918.1"
                     /translation="MNYSQKDKLYNWHPYTQHKTAANFPAIVKGKDALLWDENGKEYV
                     DAIASWWVNPFGHSNQVIADAIYKQLTTLEHVLFGGFTHNKAVELAEKLMTILPTNQK
                     KLFYSDNGSTAVEVALKAALQYNYNKGIKKTKIIAFEEAFHGDTFGAMASSGISFFTE
                     AFQGSLLQVDRIPVPTTGNEEKSIFELKRLLATNDYAAFLFEPLVLGAAGMVMYEPEA
                     LDKLIAICNDNHVFTIADEVMTGFGKTGKTFATDYLQHKPDMMCLSKALTGGTIPMAI
                     TSFTQEIFDGFCDDDTNKALFHGHTFTANPTGCAAALASINLLQTDQMQNHIRRVNES
                     HLVFENKIKNHPKVKTSRVLGVIFALEIKTESEETYYGTMRNKLYNFFIEKGIILRPV
                     GNIVYILPPYIITENQLQKVYQTIEKALEIV"
     gene            4838..5992
                     /locus_tag="PK218_10185"
     CDS             4838..5992
                     /locus_tag="PK218_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPW98919.1"
                     /translation="MKQKIAITSLASISTLGNTHETIWANYLSDKSFITTKEFNGKTE
                     FAATFPASIRSELEALRQSDSKYKSLDETVLMAIAVSRTAIEKAGWKQGDDFGINIGS
                     SRGATQLIERYHKEFLETGTTATLTSPTTTLGNISTWVAHDLKSTGVEISHSITCSTA
                     LHAILNAIAWIQSGMATKFLVGGSEAPLTNFTIAQMKALKIYANQDQDYPCQAFNLEK
                     TKNTMVLGEAASVACLELGENKKAIAYITGIGYATDTLEHSISISEEAICFQKSMKMA
                     MGDLDSSEIDAIVMHAPGTIKGDLSEYKAIQKVFGKNLPLLTTNKWKIGHTFGASGML
                     SIELAVMMLQHQEFIEVPFATPQETRKNLKNILINAVGFGGNAVSILLTR"
BASE COUNT         2120 a         1053 c         1110 g         1830 t
ORIGIN      
        1 cctgcggatg acttaaccga tccagcacca gcaacaacat ttgctcactt agatgctaca
       61 acggtattgt ctcgtaaaat tgctgagtta ggtatttatc ctgcagtaga tccattagat
      121 tctacttcaa gaattcttac tgcgcaaatc ttaggtgatg agcattatga ttgtgcacaa
      181 agagtaaaag agattttaca aaaatacaag caattacaag atatcatcgc gattcttggt
      241 atggaagaat tatcagaaga agataaactt tctgtatcta gagcacgtcg tgtacaacgt
      301 ttcttgtctc aaccgttcca cgttgctgag caatttacag gtattccagg agtattagtt
      361 gatattaaag acaccatcaa aggatttaac atgatcattg atggagagtt agaccatttg
      421 ccagaagcgg ctttcaacct taaaggaacc attgaagacg ttatcgaagc aggtcaaaaa
      481 atgttagcag aagcgtaaat caagcgaagc ttgataaact tcagaaaata aaatcacaaa
      541 tcccaatttg gaatttggaa ttttaagatt tggaatttta aagaaaaatt atgattttag
      601 aaatagtatc tccagaagca agtttgttca aaggtgaagt aacttcggtt tatttgccag
      661 gtgttgatgg ttcttttcaa atattgaaca atcacgcacc gatagtttca attttaaaac
      721 agggtaccgt taaaattact gccaacagtt ttacatttga aaaagaagtt gcagaaaaat
      781 tctctaaagt aaacgatcaa aattatactt tagaaatcaa ttctggaact attgaaatga
      841 aagacaacaa agtaattgtt ttagccgact aagacaattc aaaataaacg aagaaagctc
      901 aacaattttg ttgggctttt tttatttttg gaagatgaaa aacaacaatg aatttgaatt
      961 aatagtagcg caaggggaaa atccttggtg gaggctatta ttgtctgttg ctttttttac
     1021 tttaatggtt tgggttgttt ctgaaaatat aattatgttt tataattacg gatttcatga
     1081 agatactttt agagtatttt caaaaagtct ttttacaatt ggatattctt tgacaggcgg
     1141 aataattttt gccgtaacta aaacaatatt aattgatgtg gataaagacc ttttaatttc
     1201 tagatttaat gttgggccat tttctaaaga tgttaaatca acagttcctc aacttgaata
     1261 tgtttctata tttcttgatg ataaagaaaa ctaccaagta aacctttggt atattggaaa
     1321 taaacattat aatatgtatc aatttgatga aaaggaaatt gctatgaaat ttggaaatca
     1381 tgttgctata aaactcaact tagatttatt agacgcaact gaaagaggag ataataaatg
     1441 ggtagaaaaa attaaagtat gaaactccca aaagcacttc aatccaaatt aaaactccgt
     1501 gaagaaaata atgctttgcg ataccttccg ttgacaaata ctttagtaga ttttgcatct
     1561 aatgattatt taggctttgc aaaaaacgaa ttaatttttg atgtttcaca ccaactttta
     1621 gtaaatcaaa atttaaagat aaacggcgca acaggttcaa gattactttc aggtaatcat
     1681 agtttatatc cactcacaga agatttcatt gctcaatttc atcaatcaga agcagctcta
     1741 atatttaatt cgggttatga tgctaatgtt ggttttttta gtacagtacc gcaacgaaat
     1801 gatatgattt tatacgatga attgtgtcac gcttccattc gtgacggtat tcaaatgagc
     1861 cacgccaaag cctacaagtt tcaacacaat gattttctag atttagaaaa actaattcat
     1921 aaaattactt ccaacttcca acttccagca tccatctata ttgtaactga aagcgttttc
     1981 tcaatggatg gcgattcgcc taatttggag gaattgacca atctgtctga aaagtataat
     2041 tgttatttag ttattgatga agcgcattca ttgggtgttt ttggcagtca aggtgaagga
     2101 ttagtgcaaa gtgtagcatt gcatgaaaaa gtttttgccc gaatcatgac atttggcaaa
     2161 ggactaggtt gccatggtgc tgccatttta ggaagcaatg acctaaaaaa ctatctggtc
     2221 aatttcgctc gtagttttat ttatactacg ggactttctc cacattctgt tgcaacgatt
     2281 ttgatgagtt atcgtcaact aatagtgcaa gaagaagcac tacaaaaatt aaaaaacaac
     2341 atcattcatt tcaatcaaga gaaaatgcgt ttgggtttga aaccattatt tgtttacagt
     2401 aaatcggcaa ttcattgtgc cattatttcg ggcaatcaaa acgtgaaagc tacttcggaa
     2461 ttactacaag aaaaagggtt tgacgtaaaa cctatccttt cgccaaccgt ttccgaaggt
     2521 caagaacgat tgcgtttctg cttgcatagc tataattctc atcaacaaat aacaaaagtt
     2581 ttagaacttt taagttcctg tgtttttaaa taaaaacatt gtgaactttg cgcattcttt
     2641 gcgaactttg cggttaaata tttactatga aactattcat cacaggcatt ggtaccgatg
     2701 taggtaaaac tattgcatca tctattgtta cacaagcttt agaagcagat tattggaaac
     2761 ccattcaagc aggcgattta gagaattccg acagccataa aatccaaaag tatattagca
     2821 acaacaaaac agtaatccac gacaacagtt ataaactaaa tactcccgcc agtccacact
     2881 atgcagcgga aattgacgga ataacaattg atgtaaaaaa aataaaagaa ccaaagacag
     2941 ccaatcattt agttattgaa ggcgcaggag gaattctagt tcctataaat aataatgatt
     3001 ctattgtcaa tttaattcag cctgactata aagttattgt agtttcgcgc cattacttag
     3061 gaagcatcaa tcataccttg atgacttttg aagtgttgca aagcagaaat attaaagtag
     3121 ctggaattat cttttcgggt gatgaaaata ccgcaacaga agcaatcatt ttatcaaaga
     3181 cgaaagccaa cttcatcgga agaattgaaa acgaacctta ttttgatcat aatgtaatac
     3241 aatattatgc cgataagttc agagataatt tagaaaaact ctaaaagact agagcaaaga
     3301 aacaagaaga atgagaattt cttgtcttat ctttgcaaaa cgattttata aagagcaaaa
     3361 gtcttttctc ttttctcttt cttctatttt ctttaaaaat gaactattct caaaaagata
     3421 aactatacaa ttggcatcct tatactcaac acaaaacagc tgccaatttt ccagccatag
     3481 taaaaggtaa agacgccttg ctttgggatg aaaacggcaa agaatatgta gatgctatcg
     3541 cttcttggtg ggtcaatccg tttggacatt ccaatcaagt gattgccgat gctatttaca
     3601 aacagttgac tactttggag catgtgcttt ttggtggatt tacgcataac aaagccgtag
     3661 aattggcgga gaagttaatg accattttgc caaccaatca aaagaaactt ttctattcgg
     3721 ataatggttc aacagcggta gaagtagcat tgaaagccgc attacaatac aattacaata
     3781 aaggaatcaa aaaaactaaa atcatagcct ttgaagaggc gttccatggc gatacttttg
     3841 gagcgatggc aagtagtggc atttcttttt ttaccgaagc ctttcaaggc tcgttgctac
     3901 aagtggatag aattccagta ccaactacgg gtaatgaaga aaaaagcata ttcgaattaa
     3961 aacgcttact agctactaat gactatgctg cttttctttt tgaaccttta gtactaggcg
     4021 cagcaggaat ggtcatgtat gagccagaag ctctagataa actgattgct atttgtaacg
     4081 acaaccatgt ttttactatt gccgatgaag taatgacggg ttttggaaaa acaggaaaaa
     4141 catttgctac cgattattta caacacaaac ccgatatgat gtgtttgtct aaagcgttaa
     4201 caggtggaac tatcccgatg gcaataactt catttactca agaaatattt gatggttttt
     4261 gcgacgacga caccaacaaa gcattgtttc atgggcatac ttttacagca aatccaacag
     4321 gttgtgccgc tgctttggca agtatcaatt tgttgcaaac cgaccaaatg caaaaccata
     4381 ttcgtcgtgt gaatgaaagt catttggtgt ttgaaaataa aattaaaaac catccaaaag
     4441 taaaaacttc acgggtgcta ggcgttattt ttgctttaga aattaaaacc gagagtgaag
     4501 aaacttacta tggaacaatg cgcaacaaat tgtataattt cttcatagaa aaaggaataa
     4561 ttctgcgacc agttggaaac attgtgtaca tattgccacc ttatattata acagaaaatc
     4621 aattgcaaaa agtctatcaa acgatagaaa aagctttaga aatagtttaa atttgtagtc
     4681 taaccacaaa caacaccaag acggcacaat gtgcacaaaa ggtttgagtt atttgtggtt
     4741 ttattaaatt aattaaacca aaaagaatcc gtcgagtgcg ctaaggtttg tgttctttgc
     4801 gaaaaacttt gtgtcctttg tggttaaacc ttatatattg aaacaaaaaa ttgccataac
     4861 ttcattagct tctatctcca ccttggggaa cactcacgaa acaatttggg caaattatct
     4921 ttcggataaa tcatttatta caactaaaga attcaatgga aaaactgaat ttgcagctac
     4981 ttttccagca tcaattagaa gtgaattgga agcattgcgc caatcggatt caaaatataa
     5041 gtcgttggat gaaacggttt tgatggctat agcagtttcg cgaaccgcca ttgaaaaagc
     5101 agggtggaaa caaggcgatg attttggaat caacattggt tcctcgcgcg gagctacaca
     5161 attgatagaa cgctaccata aagaatttct cgaaacggga acgacagcta cattaacttc
     5221 gcctacaacc acattaggaa acatttctac ttgggtagcg cacgatttaa aaagcacagg
     5281 tgtcgaaatt tcccattcta taacctgttc tactgcgcta catgctattt tgaatgcaat
     5341 agcttggatt cagagtggca tggcaaccaa atttttagta ggtggaagcg aagcaccatt
     5401 gaccaatttt accattgcgc aaatgaaagc cttaaaaatc tacgccaatc aagaccaaga
     5461 ctatccgtgc caagcgttta atttagaaaa aaccaaaaac actatggtgc tgggtgaagc
     5521 cgccagtgtg gcgtgtttag aacttggcga gaataaaaaa gctatcgctt atatcacagg
     5581 cattggctat gcaaccgata ccttagaaca cagtatttct atatctgaag aagctatatg
     5641 ttttcaaaaa tcgatgaaaa tggcgatggg cgatttggac tcttccgaaa tcgatgccat
     5701 agtaatgcat gcacccggaa ctattaaagg tgatttgtcc gaatacaaag ccatccaaaa
     5761 agtatttggc aaaaacctac cgttattgac taccaacaaa tggaaaattg ggcacacctt
     5821 tggtgcctcg ggcatgctga gtatagaatt ggcggtcatg atgctgcaac atcaggagtt
     5881 tattgaagtt ccttttgcca cacctcaaga aactcgaaaa aacctcaaga acatcttgat
     5941 taatgctgta ggttttggag gaaatgcagt gagtattctc ttgacacgct aagaaaaagc
     6001 cgattttcct ataggtgaaa ccaaatctcc taaaggggaa gcccattttc ttataggaga
     6061 aacccatttt cctaaaggag aaacccattt tcttatagga gaaacccatt ttc
//
LOCUS       DAOOBH010000122         5913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_15624_length_5913_cov_5.503243, whole genome shotgun sequence.
ACCESSION   DAOOBH010000122 DAOOBH010000000
VERSION     DAOOBH010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15624_length_5913_cov_5.503243"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PK218_10190"
     CDS             <1..1016
                     /locus_tag="PK218_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW98920.1"
                     /translation="HSYILYGPLLNGATTVLFEGVPSYPNYSRFWDVIEKHQVNQFYT
                     APTAIRALAKKSIDFVQKYPLQSLKVIGSVGEPINEEAWHWYNDHVGGKRCPLVDTWW
                     QTETGGIMISPIPFITPTKPTYATLPLPGIQPVLMDELRNEIEGNQVTGSLCIKFPWP
                     SMARTIWNDHQRFKETYFTAFPGKYFTGDGALRDEVGYYRITGRVDDVIIVSGHNLGT
                     APIEDAINEHPAVAESAIVGFPHDVKGSALYGYVTLKESGESRNQDNLIKEINQLISD
                     QIGPIAKLDKIQFVSGLPKTRSGKIMRRILRKIAEGDYSNFGDTSTLLNPECVDVIIS
                     GKE"
     gene            1116..2234
                     /locus_tag="PK218_10195"
     CDS             1116..2234
                     /locus_tag="PK218_10195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW98921.1"
                     /translation="MENQSEIVIVGGGLAGLTAAIHLSKHGFSVTVIEKNEFPKHKVC
                     GEYISNEVLPYCHYLALDLEGLNVAKINQLEFSIANGTFITSNLPLGGFGVSRYTLDY
                     YLYQKAIALGCKFFQEEVQEIIFSNNNFTVFTTNEQQFKSKIVLGAFGKRSTMDVTLK
                     RDFIKKKSPWLAIKAHYNCDFPDNLVGLHNFEGGYCGVSKVENNQVNICYLAQYKSFK
                     RYKNIEDFQMQVLMKNPKLKTIFEKSNLLFEKPLTISQISFEAKKIVENHILMIGDSA
                     GLIHPLCGNGMAMAMHSAKIASELSIALLQEKISRTDFEKHYQEKWNETFKSRIRLGK
                     LLSKVLLRPTLSNIIMKIMVLFPFSFPLLIKKTHGKTF"
     gene            2235..2951
                     /locus_tag="PK218_10200"
     CDS             2235..2951
                     /locus_tag="PK218_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPW98922.1"
                     /translation="MKLNTTYRSTEDEIMDDFSLEGEELRQALDKIAKINQLLGGNKV
                     ILDGIKELLRDIPKKQQITIVDVGCGNGDMLRALAKFGKNKDLNLNLIGIDANQFTID
                     YAEKLSKEYKEISYRCEDVFKADFEKLKYDIVLCTLTLHHFTEDKILDLLAVFTKKAS
                     VGIIINDLHRSKIAYRLFQLICFVFQLNTMSKEDGLTSILRGFKKEELHIYSKKMNFK
                     AYSIHWKWAFRYQWIIRSMR"
     gene            2987..4039
                     /locus_tag="PK218_10205"
     CDS             2987..4039
                     /locus_tag="PK218_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HPW98923.1"
                     /translation="MSVKIVNVATQLPDFYRETKDILPFLDVWLANHDDRFKRKVKKI
                     FEGANVDKRYSIMSPEEVFSLHSFEERNAIYSREMIVLGEKVLIKALQKANWKPETLD
                     YIITVSCTGIMIPSLDAYLINSLQLKQDIVRLPVTEMGCVGGVSGIIYAKNFLKANPN
                     KRAAVITVESPTATFQLEDYSMANIVSAAIFGDGAACVLLSSHQEDVGPTILDESMYH
                     FYDKEHMMGFELTNKGLKMVLDIEVPDTIEEHFPSILIPFLKRNQLEIKDIDHLIFHP
                     GGKKIVNLVEGLFSEFDKNIDDTKEVLRLFGNMSSATVLYVLERMMNTPKQKSEKGLM
                     LSFGPGFTAQRVLLEF"
     gene            4045..4473
                     /locus_tag="PK218_10210"
     CDS             4045..4473
                     /locus_tag="PK218_10210"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HPW98924.1"
                     /translation="MTSREILSKLPYSKPFLFVDELTVISENGAEGTFTFDEGLDFYK
                     GHFKNNPITPGVILIETMAQIGLVCLGIYLGKDNNNTETKFAFTSSEIEFLKPVFPNE
                     KVTVISEKVYYRFGKLKCKVSMRNSKNEEVCSGTISGMIL"
     gene            4561..5829
                     /locus_tag="PK218_10215"
     CDS             4561..5829
                     /locus_tag="PK218_10215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPW98925.1"
                     /translation="MKKRVVITGLGIVSPNGIGLDSFTQAIKNGISGIKHDAELERLK
                     FSCQIAGKPEVSTELSLQYFTDLELRNFNATGILYGVIAGMDAWQDAGLSIENNLEPD
                     WDSGTIFGTGTSGIDKFRESIYKIDDLQTRRLGSTAVAQTMNSGISAYLGGKLGLGNQ
                     VTTNSSACTTGTESIVMGFERIQQGKAKRMLVGSTSDSGPYIWGGFDAMKVCTFKHND
                     SPENGSRPMSASASGFVPSSGSGAMVIEELETALARGAKIYAEILGGNLNSGGQRGDG
                     TMTAPNATAVQKCIKDALLDAEIHPNEIDAINGHLTATAKDAFEIENWCKALNRSGTD
                     FPYINSLKSQVGHCLSGAGSVESVATVLQIHHGFIFPNRNCDDLNEDIVKRIAPEKIP
                     QVFMNKQINIVVKASFGFGDVNGCIVFKKY"
BASE COUNT         2020 a          947 c         1163 g         1783 t
ORIGIN      
        1 gacattccta tattctttac ggaccattat taaacggtgc tacaacagtt ctttttgaag
       61 gcgttccttc ttatcctaat tacagtcgtt tttgggatgt tatcgaaaaa catcaagtca
      121 atcagtttta tactgcgcca accgctatca gagctttggc gaaaaagagc atcgactttg
      181 ttcaaaaata tcctttacaa tcattaaaag tgattggttc cgttggtgag cctattaatg
      241 aagaagcttg gcattggtat aatgaccatg ttggcggaaa acgttgccca ttagtagata
      301 catggtggca aaccgaaact ggcggcatta tgatttctcc aattccattc attacaccaa
      361 ccaaaccaac gtatgcaacg ttgcctttac ccggaataca accagtgttg atggatgaat
      421 tgcgtaacga aatcgaaggc aaccaagtaa cgggtagttt gtgtatcaaa ttcccatggc
      481 caagtatggc acgaaccatt tggaatgacc atcaacgttt taaagaaact tattttactg
      541 cttttccagg aaaatatttt actggtgatg gtgctttgcg tgatgaagta ggctattaca
      601 gaattacagg tagagtagat gatgtcatca ttgtttcagg acataattta ggaacagcac
      661 ctattgaaga tgctatcaac gaacatccag cagtagccga aagtgctatc gttggctttc
      721 ctcacgatgt gaaagggagt gctttatacg gttatgtaac cctaaaagaa tcaggcgaaa
      781 gtagaaatca agacaatctg attaaagaaa tcaatcaact aatatcagac caaattggtc
      841 caatcgctaa actagataag atccaattcg tatcaggttt acccaaaacg cgttcgggaa
      901 aaatcatgcg acgaatatta cgaaaaattg ctgaaggtga ctatagtaac ttcggagaca
      961 cttccacatt gctcaatcca gagtgtgtag atgttataat ttcaggaaaa gaataagatt
     1021 ttaaaaactg cttcaccaag cagttttttt gattaaacaa ttgttaattc tagtttagaa
     1081 tctttttata attttaaatt gattactttg gtagggtgga aaatcaatct gaaatagtca
     1141 tcgttggtgg tgggttggct ggacttacag cggccatcca tctttctaaa catggctttt
     1201 cggttactgt tatcgaaaaa aatgaatttc caaaacacaa agtctgtggc gaatatatct
     1261 ccaatgaagt gctgccatat tgccactatt tagctctcga tttagaaggg ttaaacgttg
     1321 caaaaatcaa ccaacttgaa ttttctattg ctaacggaac tttcattact tccaatcttc
     1381 cattaggtgg atttggggta agtcgctaca ctttagatta ttatttatac caaaaagcga
     1441 ttgcacttgg ttgtaaattt tttcaagaag aagttcaaga aataattttt tcaaataata
     1501 attttaccgt ttttactact aacgaacaac aattcaaatc taaaatagtt cttggagctt
     1561 tcgggaaacg ttctacaatg gatgtaactt tgaaaagaga ttttattaaa aaaaaatcac
     1621 cctggttagc cataaaagcc cattacaatt gcgattttcc tgataattta gttggattac
     1681 ataattttga aggcggttat tgtggcgttt ctaaagtaga aaataatcag gtaaatattt
     1741 gttatctggc acaatataaa tcctttaaaa ggtataaaaa tattgaagat tttcagatgc
     1801 aagttttaat gaagaatcca aaattgaaaa ctatttttga aaagagtaac ctattatttg
     1861 aaaaaccttt aaccattagc caaatttctt ttgaagccaa aaaaatcgtg gaaaatcata
     1921 tattaatgat aggcgactcg gcgggattaa ttcatccttt gtgtggcaac ggaatggcga
     1981 tggcaatgca cagcgcaaaa attgcatccg aactttcgat agcattgctt caagaaaaga
     2041 tttcacgaac tgactttgaa aaacactatc aagaaaaatg gaatgaaacc tttaaaagca
     2101 gaattcgatt gggtaaattg ctgtcgaaag tattgttaag accaacgtta tctaatataa
     2161 taatgaaaat aatggtactt ttccctttca gtttcccttt attaatcaaa aaaactcatg
     2221 gcaaaacttt ctaaatgaaa ctaaacacaa cctaccgttc gaccgaagat gaaattatgg
     2281 atgatttttc attagaaggg gaagaattgc gtcaagcttt ggataaaatt gctaaaataa
     2341 accagctttt aggcggaaat aaagtaattt tagatggaat aaaagaattg ctaagagata
     2401 ttcctaaaaa gcaacaaatt actattgttg atgtgggttg tggaaacggc gacatgctgc
     2461 gtgctttagc aaagtttgga aaaaataagg atttgaattt aaacttaatc ggaattgatg
     2521 ccaaccaatt taccatcgat tatgccgaga aattatcaaa agaatacaag gaaataagct
     2581 atcgttgtga agatgttttt aaagctgatt ttgaaaaatt aaaatacgat attgttttat
     2641 gcacattaac tttacatcat tttaccgagg ataaaatact agatttattg gcggttttta
     2701 ctaaaaaagc aagtgttgga ataattatta atgatttgca tcgcagtaaa attgcctatc
     2761 gtttgtttca actaatttgt tttgtatttc aattgaatac gatgtcaaaa gaagatgggt
     2821 taacgtcaat tttaagagga tttaagaaag aagaattaca tatttattct aaaaaaatga
     2881 attttaaagc gtattctatt cattggaaat gggcatttcg ttatcaatgg attattcgaa
     2941 gtatgaggta agttattcga ggtaaaataa aaaaaacaaa aaataaatgt ctgttaaaat
     3001 tgtaaatgtt gccacccaat tgcctgattt ttatcgggaa acaaaagaca tcttgccgtt
     3061 tttggatgtt tggttagcca atcatgacga ccgatttaaa cgtaaagtga agaaaatatt
     3121 tgaaggcgcc aatgtggaca aacgttattc catcatgagt cctgaagaag ttttttcact
     3181 acattctttt gaagaacgaa acgccatata ttctcgagaa atgattgtac ttggcgaaaa
     3241 agtattaatc aaagcgttgc aaaaagccaa ttggaaaccc gaaacattgg attatatcat
     3301 taccgttagt tgcaccggaa ttatgatacc ctctttggat gcttatctga tcaattcatt
     3361 gcagttaaaa caagatatag tgcgacttcc cgttaccgaa atgggttgcg ttggcggtgt
     3421 ttcgggtatc atttatgcca aaaactttct aaaagccaat ccaaacaaac gcgcggcggt
     3481 gattacagtt gaaagtccaa ctgccacttt tcagttagag gattattcca tggcaaacat
     3541 tgtcagtgca gctatttttg gcgatggtgc agcctgtgtt ttattgtctt cacatcaaga
     3601 agatgttggt ccgactatcc tagacgaatc gatgtatcat ttttatgaca aggaacacat
     3661 gatgggcttt gagctaacaa acaaaggact aaaaatggtt ttggacatcg aagttcctga
     3721 tactattgaa gaacattttc catcaatttt aattccgttt ttaaaacgaa atcaattaga
     3781 gataaaagat attgaccatt tgatatttca tccaggtggt aaaaaaatcg taaatttggt
     3841 agaaggtttg ttctctgaat ttgataaaaa tatagacgat actaaagaag ttttacgctt
     3901 gttcggaaac atgtcaagtg ctaccgtgct atatgtttta gaacgcatga tgaatactcc
     3961 aaagcaaaaa agcgaaaaag gattgatgct tagttttggc ccaggtttta cagcgcaacg
     4021 tgttttatta gaattttaac aaagatgact tcaagagaga tactttcaaa attaccgtac
     4081 tccaaaccat ttttgtttgt ggatgaacta actgttattt ctgaaaacgg agccgaagga
     4141 accttcacat ttgatgaagg attggacttt tataaaggtc attttaagaa caatccaatt
     4201 actccaggcg ttattttgat agaaacgatg gcacaaatcg gattggtttg tttagggatt
     4261 tatttaggaa aagacaacaa caatactgaa acgaaatttg catttacctc aagtgaaata
     4321 gagtttctta aacctgtttt tcctaatgaa aaagtaaccg ttatttccga aaaagtatat
     4381 taccgatttg gaaagttaaa atgcaaagtg agtatgcgca acagcaaaaa tgaagaagtt
     4441 tgttcaggaa caatttcggg gatgatttta taagaattgt taaaagttta taggtttaag
     4501 agtttgaaat tgtttgaatc ctgaaataat aacttaatta acctaaatgg tttaaaaata
     4561 atgaaaaaaa gagttgtaat aacaggttta ggaatcgttt ctcccaatgg cattgggtta
     4621 gacagtttca cgcaggctat taaaaacggc atttcgggta taaaacacga tgccgaattg
     4681 gaacgcttga aattctcctg ccaaatagca gggaagcccg aagtttcaac cgaactttct
     4741 ttacaatatt tcaccgattt agaacttcga aactttaacg caactggaat tctctatggc
     4801 gttattgcag gaatggatgc ttggcaagac gcaggtttat ccattgaaaa caatcttgaa
     4861 cccgattggg atagcggcac tattttcgga acaggaacat caggaattga taagtttaga
     4921 gaaagtattt ataaaataga cgatttgcaa acccgacgat tgggaagtac tgccgtggcg
     4981 caaaccatga acagcggaat atcagcctat ctcggcggaa aactaggttt aggaaaccaa
     5041 gtaacgacca attcctctgc ttgtacaacg ggaaccgaaa gcatagtaat gggttttgag
     5101 cgcattcaac aaggcaaagc caaacgaatg ttggtaggaa gcacgagcga ttctggacct
     5161 tacatttggg gtggatttga cgctatgaaa gtgtgcactt ttaaacacaa tgattcgccc
     5221 gaaaacggct cccgaccaat gagtgcttcg gcctcggggt ttgtgcccag tagtggttca
     5281 ggtgcgatgg tgattgaaga actcgaaacg gcactagcgc gtggtgcaaa aatatatgcc
     5341 gaaattcttg gaggcaattt aaattcgggt gggcaacgtg gcgatggaac gatgaccgca
     5401 ccaaacgcaa ctgccgttca gaaatgtatt aaagacgcat tgctcgatgc cgaaattcat
     5461 cccaatgaaa ttgatgccat caacggacat ttaaccgcaa cagcaaaaga cgctttcgaa
     5521 atagagaatt ggtgcaaagc tttgaacaga agtggaacgg attttccgta tatcaattct
     5581 ttaaaatcgc aagtgggtca ttgtctttcg ggtgctggaa gtgtcgaatc ggtggcaacg
     5641 gtactgcaaa ttcatcatgg ttttattttt cccaatagaa attgtgacga cttaaatgaa
     5701 gacattgtaa aacgaattgc tcccgaaaaa attccacaag tatttatgaa taaacaaatt
     5761 aatatcgttg ttaaagcaag ttttggtttt ggcgatgtta atggatgtat tgtttttaag
     5821 aaatattgag tattaagatt ggagtattaa gtagtaagac tagagtatta agttgtaaga
     5881 ttgaagtaag tttagactaa aaataaataa atg
//
LOCUS       DAOOBH010000123         5694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_16329_length_5694_cov_4.325590, whole genome shotgun sequence.
ACCESSION   DAOOBH010000123 DAOOBH010000000
VERSION     DAOOBH010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16329_length_5694_cov_4.325590"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>531)
                     /locus_tag="PK218_10220"
     CDS             complement(<1..>531)
                     /locus_tag="PK218_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98926.1"
                     /translation="FDTDNPTNYQRYFDDFNISTLLKDKEGNHWIGTLGHGLLLVPNF
                     KTYLIPTTDTPSKLVYANKKLVFSTQNDKIYSSVDEKNPFVFRPLYEGKSNHTIEQFY
                     VDTIHQKMLFTSNTFKTTDLKGTVLKEVVLASKEFIPIDNTYYGYASSGACGLFKVSD
                     ERSSWDLIFASNEIST"
     assembly_gap    532..541
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<542..1303)
                     /locus_tag="PK218_10225"
     CDS             complement(<542..1303)
                     /locus_tag="PK218_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPW98927.1"
                     /translation="MRKIVFLFVLISFQFFGQNPHYFTIDKTKGLPSNTIYDIYQDKN
                     DMLWIATNEGICSFDGKKFTTFSSTVQVSKAGSNIVEDVYGRIWYCSFDGYIYYIENG
                     VLKSLRQQKPLGYQKFAIIKDCFVYLEENKLIFLDLKTLKPIKTIALATEKTTAMHTF
                     KGFYYLYATDFLYEISSVNDVKKIEVPEKIKKNFGVSIISNTNDKLLFVSKYSNKYCL
                     YVDGVFKEYDFKSTINFIQNTSYSSNTNWIATTKGI"
     gene            1682..3184
                     /locus_tag="PK218_10230"
     CDS             1682..3184
                     /locus_tag="PK218_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98928.1"
                     /translation="MKTKLLLLLLLAFSFANAQLMVPSVGVNTITNITQTTATVNYAI
                     RTNGTYTNARIEHSLVSNMSTLIAQSSFIGFSNNGAWQYASYNLSGLVPNTTYYVRAV
                     ASNSLGTTTSNILSFTTVGNIIPDITAVGVGVTENSATINYSLNANLSATTSIVKYGT
                     SSDALNSQVSGFSATGNTTSAGSALITGLQQNTQYFYQIEAINSFGTALSSGSFQTAL
                     PPQILANYTFDNTLSDVNGANAFELQAGMTYGTDRSGVANKALFINGTGTTVSLTNLP
                     VGNSSRTFSFWIKPTQSNGSNRIFSYGSPSGTAAYGASFDATRVYNFSWTTNLSFLQS
                     TSLNVWKHIVCTYEQSTSTASLYTDGVLRASVTANWSTANNGIFYLGSLFGETGSRYI
                     GFLDDLQIYNYALTGAEITSLYTSNTLSSLNFSQNNLEVALYPNPTKEVLNIQMTNEV
                     KTVEIFNLQGQKVVTSNQKQIIVSNLSRGLYMVKIEDIKGNTASKKVVFE"
     gene            3546..4595
                     /gene="queA"
                     /locus_tag="PK218_10235"
     CDS             3546..4595
                     /gene="queA"
                     /locus_tag="PK218_10235"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPW98929.1"
                     /translation="MKLSHFNFNLPVELLAEYPAENRDESRLMVVNRQTKTIEHRLFK
                     DVIDYFDDGDVFIINNTKVFPARMYGNKEKTGARIEVFLLRELNAEQRLWDVLVDPAR
                     KIRIGNKLYFGDDDSLVAEVIDNTTSRGRTLRFLYDGSYEDFRMKLLELGETPIPKYI
                     NRELTAEDAERYQTIYAKEEGAVAAPTAGLHFSKHLLKRLEIKGIDFAEVTLHVGLGT
                     FNPVEVEDLSKHKMDSEELKIKQEACDIVNNAKLKKKKICCVGTTSMRAIESSVSSQG
                     TLNPFEGWTNKFIFPPHDFSIADCMITNFHTPKSTLLMMVSAFCGHDLMKKAYEEAVK
                     EKYKFYSYGDAMLII"
     gene            4884..>5694
                     /gene="kynU"
                     /locus_tag="PK218_10240"
     CDS             4884..>5694
                     /gene="kynU"
                     /locus_tag="PK218_10240"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HPW98930.1"
                     /translation="MTFQNTLEFAAELDRQDELNKYRNEFIFPQHEGKDVIYFTGNSL
                     GLQPKSAKKYVNEVMDDWANLAVEGHFFAEKPWWDYHERFAEPLSKIVGAKPSEVTVM
                     NTLTVNLHLLMVSFYKPTAKRYKIICEEKAFPSDQYMFQSQVNFHGYKPEDTIVEIKR
                     RDGEHNIRLEDILTKIEEVGDELVLVLFGGVNYYTGQIFDMKTITEAAHKVGAIVGFD
                     LAHAAGNIKLELHSWNVDFAAWCSYKYMNSGPGNASGCFIHEKHHSSDLPRF"
BASE COUNT         2090 a          949 c          835 g         1810 t
ORIGIN      
        1 ttagttgaaa tttcatttga agcaaatatt aaatcccaac tacttctctc atcagaaact
       61 ttaaacaatc cacaagctcc actcgaagcg tatccataat aagtgttgtc aattggtata
      121 aactcctttg atgccaaaac aacttctttt aaaacagttc ctttcaaatc tgttgtttta
      181 aaagtgtttg aagtaaaaag cattttttga tgaatggtat caacataaaa ctgctcaata
      241 gtatgattag attttccttc atataaaggt ctgaaaacaa aaggattttt ttcatcaaca
      301 cttgaatata ttttgtcatt ctgagtgcta aaaacaagtt tcttattagc atataccaat
      361 ttagaaggtg tatctgtcgt tggaattaaa taggtcttaa aatttggaac taataataaa
      421 ccgtgtccca aagtcccaat ccaatgattg ccttctttgt cttttaataa agttgaaata
      481 ttaaaatcat caaaataacg ttgataattt gttggattgt cagtgtcaaa tnnnnnnnnn
      541 naattccttt cgtagttgca atccaattag tattactgct ataactagta ttttgtataa
      601 aattaatagt gcttttaaaa tcatattctt taaaaacacc atccacatac aaacaatatt
      661 tgttagaata cttagagaca aaaagtaatt tgtcgttcgt attacttata atactaacac
      721 caaagttctt tttaatcttc tccggaactt ctattttttt tacatcgttt acactactta
      781 tttcgtataa aaaatcggta gcatataaat aataaaaacc tttaaaagtg tgcatcgcag
      841 tagttttttc ggtagcaaga gcaatggttt taataggttt taaagttttc aaatctagaa
      901 atatcaattt gttttcctca agatatacaa aacaatcttt tataattgca aatttttgat
      961 aacccaaagg tttttgttgc cttaaagact tcaatacacc attttcaata taataaatat
     1021 acccatcaaa agagcaatac caaattcgcc cataaacatc ttccacaata tttgaacctg
     1081 cttttgaaac ctgaacagta ctagaaaaag tggtgaattt tttaccgtca aaactacaaa
     1141 taccttcatt ggtagcaatc caaagcatgt cattcttgtc ctgataaata tcatagatag
     1201 tgtttgacgg caatccttta gttttatcaa tagtaaaata atgaggattt tgcccaaaga
     1261 attgaaagga aatcagaaca aataaaaaga caatttttct caaagaataa tatttaaaac
     1321 aaatgtaatt aaaaacgaat gtaaattaat agctagttca caaaacacgt ttttcaattt
     1381 gtgaaagcta tttttacctt tcacaaactc aaaacagcct ttcataaact acaccaatat
     1441 tttaaataaa accagcctaa ttttgtttca atattaattc taacatcatg aaacacatag
     1501 catcaaaaat caacaattac cgtttagaaa ttgtaataat atcaatttat actttggctt
     1561 caatactcgt ttttaattgg gttttctagt ttcacaaacc caaaacacca gttcataaac
     1621 tcatcaacaa aataaagaca atccaaacct acttttgaac caaactttaa aactaaatat
     1681 tatgaaaaca aaattacttt tacttttatt attagctttt tcttttgcta atgcacaatt
     1741 aatggttcca agtgtaggtg taaatacaat tacaaacata acccaaacaa ctgcaacagt
     1801 aaattatgca ataagaacca atggaactta cacaaatgct agaattgaac attcactggt
     1861 ttcaaacatg agcacattaa tagcacaaag tagttttatt ggcttttcta ataatggagc
     1921 atggcagtat gcatcatata atttgtctgg gttagttcct aatacaactt attatgttcg
     1981 tgctgtagca tccaattctc ttggtactac aactagtaat attcttagct tcacaacagt
     2041 cggaaatata attccagata tcactgcagt aggtgtagga gtcacagaaa actcggcaac
     2101 tataaattat tccctaaatg caaacttatc cgcaacaaca tctattgtaa aatatggaac
     2161 tagttcagat gctctaaatt ctcaagtatc aggtttttct gctacgggaa acacaacaag
     2221 tgcaggaagt gcgttaatta ctggtcttca acaaaacaca caatattttt accaaatcga
     2281 ggcaataaat tcttttggaa ccgctttaag tagtggaagt ttccaaactg ctcttcctcc
     2341 acaaatttta gcaaattata catttgacaa tacattgagc gatgtaaatg gagccaatgc
     2401 ttttgagtta caagcgggaa tgacatacgg tacagataga tcaggtgtcg caaataaagc
     2461 attattcatt aatggtacag gaactacagt atcacttact aatcttccag ttggtaatag
     2521 ctctagaact ttttcttttt ggattaaacc tacacaaagt aatggttcta ataggatttt
     2581 ttcttatggt tcaccatcag gcaccgcagc ttatggagct tcatttgatg caactagagt
     2641 ttacaacttt agctggacaa ctaatctgtc ttttcttcaa tccactagtt taaatgtttg
     2701 gaaacacatt gtttgtactt acgagcaatc aacaagcaca gcaagtttat atacagatgg
     2761 tgttctaaga gcttcagtca ctgctaattg gagtacagca aataatggta tattttattt
     2821 aggatctcta tttggtgaaa ctggttctag atatattgga tttcttgacg atttacagat
     2881 ttataattat gctttaacag gtgcagagat aacaagttta tacacaagca acaccttatc
     2941 atcattaaat ttctctcaaa acaacctaga agttgcatta tacccaaacc caacaaaaga
     3001 agtgttaaat atacaaatga caaacgaagt taaaacagta gaaatcttta accttcaagg
     3061 acaaaaagta gtgacatcaa accaaaaaca aataatagtc tctaatttat cccgtggttt
     3121 atatatggtg aaaattgaag atataaaagg taatacagca tccaaaaaag tggtatttga
     3181 gtaaaagaaa tttttagttt tttttaaaaa gtacaatcta gaaatggatt gtactttttg
     3241 tttaaaattt atttatatgc ttagaattgt tttgttaaaa atcaaacttg aaatttcaat
     3301 tgtgataatt tatacaatta gttgtatctt gatttttagg tttttattat tatacgacgc
     3361 ggtttatttg aattcgttat gtcaatttat tgctaaacag taataaaata agagttttta
     3421 aaaataaacc ttgttttact tgacaacgcc cttctcacaa tagtatattt gcagtccaaa
     3481 gattttaggc tttttaagaa acaatcttaa acacttaaac acttaaactt ataaacacat
     3541 atttaatgaa attatcacat tttaatttta atcttcctgt cgaactatta gccgaatatc
     3601 cagccgaaaa cagagacgaa tcacgtttaa tggtagtcaa cagacaaaca aaaaccatcg
     3661 aacacagact gttcaaagac gttatagatt actttgacga tggagatgtt ttcatcataa
     3721 acaacacgaa agttttccct gctcgcatgt atggaaataa agaaaaaaca ggtgccagaa
     3781 ttgaagtgtt tttacttaga gaattaaatg cagaacaacg actttgggat gttttggttg
     3841 atccagcaag aaagatacgt atcggaaata aattatattt tggtgatgat gattcattag
     3901 tcgcagaggt aattgacaat actacatctc ggggtagaac tttacgcttt ctttacgacg
     3961 gctcatacga agacttcaga atgaaactgc ttgaacttgg agaaacacca atcccaaaat
     4021 acatcaatag agaattaaca gctgaagacg cagaacgtta ccaaacaata tatgccaaag
     4081 aagaaggagc agtagccgca ccaacagctg gtttgcattt ttctaaacat ttgttaaaaa
     4141 gacttgaaat aaaaggaatc gacttcgcag aagtaaccct acacgtagga ttaggaacct
     4201 tcaatccagt tgaggtggaa gacctctcaa aacataaaat ggattctgaa gagctaaaaa
     4261 tcaaacaaga agcctgtgat attgtaaaca acgccaaact taaaaagaaa aaaatatgtt
     4321 gcgttggcac aacatctatg cgtgctatcg aaagttcagt ttcttcccaa ggcacactaa
     4381 atccttttga aggctggacg aataaattta tttttccacc acatgatttt agtattgcag
     4441 attgcatgat taccaatttt catacaccaa aatcaaccct cttaatgatg gtctcagctt
     4501 tttgtggaca cgatttaatg aaaaaagcat acgaagaagc tgtaaaagaa aaatacaaat
     4561 tctattccta tggcgatgct atgttaatta tctaaaatag cacacacaaa aatacaataa
     4621 gatctcatca gcaatgatga gatttttttt gaagcaaaat gtcagcactt tcaataaacc
     4681 ctattctgct gtctattaca atctgttgtt tggtttttat gctaaataaa agaatttaca
     4741 cactatcagg gttaaacaac caaagtcttt agtgaaatca aaaaccaaaa acaacatcca
     4801 aacaatccac tacaactttt aaacctttaa acttttaaac ctttaaactt ttcaatactt
     4861 ttacaaatca aaataaaaac acaatgactt ttcaaaacac acttgaattt gcagcagaat
     4921 tagaccgtca agacgaatta aataagtaca gaaatgagtt tatatttcca caacacgaag
     4981 gtaaagatgt tatttatttc actggaaatt cattgggttt acagccaaaa tcagccaaaa
     5041 aatatgtgaa tgaagtaatg gatgattggg caaacctagc cgttgaaggt catttttttg
     5101 ccgaaaaacc ttggtgggat tatcacgaac gttttgctga gccattaagt aaaatagtag
     5161 gagctaagcc ttcagaagtt actgtaatga atactttaac agtaaatctt catttattaa
     5221 tggtttcatt ttataagcca actgcaaaac gatataaaat tatttgtgaa gaaaaagctt
     5281 ttccatcaga ccaatacatg tttcaatcac aagtaaattt tcatggctac aaacccgaag
     5341 atacaattgt agaaattaaa cgccgagacg gagaacataa cattcgatta gaagatattt
     5401 taacaaaaat tgaagaagta ggagacgaac ttgttctagt tctttttggc ggagtaaact
     5461 attacactgg tcaaatattt gatatgaaaa ctatcaccga agcagcacat aaagttggag
     5521 caatagttgg tttcgatttg gctcatgctg ctggaaatat taaattggaa ctacacagtt
     5581 ggaatgtaga ttttgcagct tggtgttcat ataaatacat gaattctgga ccaggaaatg
     5641 cttcgggttg ttttattcat gaaaaacatc atagttcaga tttaccaaga tttg
//
LOCUS       DAOOBH010000124         5673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_16398_length_5673_cov_5.032218, whole genome shotgun sequence.
ACCESSION   DAOOBH010000124 DAOOBH010000000
VERSION     DAOOBH010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16398_length_5673_cov_5.032218"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1133
                     /locus_tag="PK218_10245"
     CDS             129..1133
                     /locus_tag="PK218_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidylprolyl isomerase"
                     /protein_id="HPW98931.1"
                     /translation="MNKVLKSLLLLFGFIIVTSCSKSDNAALEPLRDYEVQKATDMNN
                     IETFMKTHYLTVTNSPGTEDDMDVTYTPIPSGGTQVSIWDQTDYPKQTRTVTVRQGEN
                     DVVYTIYYLLLREGVGTNSKSPCNVDNVLTGYRGEFLYTKTETVGENTTTSIAGTQFE
                     ENKNPSSFFSLTGVIRGWSEIFPKLKTGSYLENPDGTISYANFGAAVMFVPSGLAYYR
                     GSQGTIPAYSPLVFSVKLFEIQRTDQDGDGIYSYQEDINNDGYVVTLPTGVINPDDTD
                     GDGIPDFQDSDDDNDGYLTRYETRINVLETATYPFDDIPTCGTSGNGKKRYLDPTCHN
                     "
     gene            1152..1925
                     /locus_tag="PK218_10250"
     CDS             1152..1925
                     /locus_tag="PK218_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPW98932.1"
                     /translation="MELKLNRPICFFDLETTGVDVARDRIVEISILKIFPNGNKESKT
                     WLVNPTIPIPIHATLVHGISDEKVANEPTFAELSSQVYNMIKDSDLAGFNSDRFDIPL
                     LAEELLRANVDFEMKNRVSVDIQTIFHKKEERTLSAAYKFYCNESLENAHSAEADTNA
                     TYEILKAQLDRYEDLENDMNFLSKFTTRKKSVDFAGFIALNEDGNEIFNFGKHKGNLV
                     EDVFDAEPGYFGWIQNADFPLYTKKVLTAIKLRKLSTKQ"
     gene            1947..2558
                     /locus_tag="PK218_10255"
     CDS             1947..2558
                     /locus_tag="PK218_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPW98933.1"
                     /translation="MKIICIGRNYVDHIEELNNERPAEPVVFLKPDTAILPKKTPFTI
                     PEFTNEVHHELEIIVKINKVGKYINKKFAHKYYDEIGLGIDFTARDIQNSLKEKGLSW
                     EKSKAFDGSAIIGDFIPKNSLDSLENIKFELTNNQNIVQEGNTNLMIWSIDELIEHVS
                     QYFTLKKGDIIFTGTPKGVAKVAPNDVLEGFLNNKKLLRLQIK"
     gene            2558..2902
                     /locus_tag="PK218_10260"
     CDS             2558..2902
                     /locus_tag="PK218_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPW98934.1"
                     /translation="MAINYNLAKVYELSDNDPEFVMQIISLFVTEVPEDMKYIEQGIE
                     IKDHKLAYSYAHKIKPTLDLLGMNQAFEEILQVEAWTKREGKRKEIDATYDSIKDQVE
                     KAIKEIKKDFEI"
     gene            3074..4537
                     /locus_tag="PK218_10265"
     CDS             3074..4537
                     /locus_tag="PK218_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW98935.1"
                     /translation="MDKTQKSSLFKVISASSMGTLIEWYDFYIFGSLAIVISTKFFPS
                     ENPTAAFLSTLATFAAGFVVRPFGALFFGRLGDLIGRKYTFMATLLLMGGATFLIGCV
                     PSFETIGYAAPILVLILRLLQGLALGGEYGGAATYVAEHAPKGQRGYFTSWIQTTATV
                     GLFISLMVILITKNVLSDSQFDAWGWRVPFWVSIVMVFVSYMIRKKMHESPEFAKAKA
                     EGNISKNPLKDSFGNKYNLKFVLLALFGATMGQGVIWYTGQFYAMSFMKIVLAIDSSQ
                     VDTLLGIALLFGTPFFVVFGWLSDKIGRKSIMMFGMLLAILLYRPIYKTMYETVNVAH
                     KELVSKKLITEGNNVITYKVYADKTEVKEVQNQTDLTIKTQMTISNHDRWMLVFLVFI
                     QVIFVTMVYGPIAAFLVEMFPTKIRYTSMSLPYHVGNGIFGGLLPAISTYFVTNAKTN
                     GNPEYYLEGLWYPIIIASVCFVIGMIYLSWKNETTND"
     gene            4547..4765
                     /locus_tag="PK218_10270"
     CDS             4547..4765
                     /locus_tag="PK218_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98936.1"
                     /translation="MNTIKRILGILWLVLAVASAYFCIFVFGLPKLTTGKTEDIVFGG
                     IIVFILAPLIVFGLSLFGYYSIKGEYDN"
     gene            4786..>5673
                     /locus_tag="PK218_10275"
     CDS             4786..>5673
                     /locus_tag="PK218_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW98937.1"
                     /translation="MSYYKIHDLENYFKMYKKSVREPKKFWDKIADENFSWYQKWEKV
                     FDFDFEQAKFRWFIDAKLNITKNCIDRHLARRGDKTAIIFEPNNPEEEAQHITYTELY
                     ARVAKMANVLTDQGIKKGDRVCIYLPMIPELAVSILACARIGAIHSVIFAGFSASAVS
                     SRINDSDCKLVITSDGGYRGDKTIDLKGIIDEALQKTPGVERVLVVKRTHANITMKEG
                     RDVWLQPLLDTAIPNNVAAIMDAEDPLFILYTSGSTGKPKGMVHTTAGYMVYTAYTFK
                     NIFNYEENDVYWCTADIGWI"
BASE COUNT         1992 a          934 c         1044 g         1703 t
ORIGIN      
        1 ttcgggtttt gtaataacga atacaccaca agtatttatc aactctcatc ttttttctaa
       61 aatcaacgtt aattagttta caaaaataaa tcaatattgt atcttgcaca cctaaaaaaa
      121 aaattaaaat gaataaagtt ttaaaatcat tactattact tttcggattt ataatagtta
      181 cttcttgttc aaaaagtgat aatgctgctt tggagccatt aagagattat gaggtgcaaa
      241 aagctactga tatgaacaat attgaaactt ttatgaaaac acattatttg acggttacca
      301 acagtccagg taccgaagat gatatggatg ttacttatac accaattcct tctggcggaa
      361 cgcaagtatc aatttgggat cagactgatt acccaaagca aacaagaaca gtgaccgtta
      421 gacagggtga aaatgatgta gtttatacga tttattatct actcttaaga gaaggagtag
      481 gaacaaatag taaatcacca tgcaatgtag ataatgtatt aacaggctat agaggtgaat
      541 tcttatatac aaaaacagaa actgttggtg aaaatacaac caccagtata gcaggaacac
      601 aatttgaaga aaataaaaat ccatcatcat tcttttcttt aacaggagtt atcagaggat
      661 ggagcgagat tttcccaaaa ttaaaaaccg gaagctattt agaaaatccc gatggaacta
      721 ttagctatgc taactttggt gctgcagtaa tgtttgttcc ttctggatta gcatattata
      781 gaggttcaca aggaacaatt ccggcttatt ctcctttggt ttttagtgta aaattatttg
      841 aaatacaaag aacagaccaa gatggagatg gaatttattc ttatcaagaa gatataaata
      901 acgatggtta tgtagtaaca cttccaacag gagtaataaa tccagatgat acagatggtg
      961 atggtattcc agactttcag gattcagacg atgataatga tggttatcta acacgttacg
     1021 aaacaagaat aaatgtactt gaaacggcta catatccttt tgatgatatt ccaacatgcg
     1081 gaacatcagg aaatggaaag aaaagatatt tagacccaac atgtcataat taaaaaacaa
     1141 acaactaaca gatggaattg aaattaaacc gacctatatg cttttttgat ctcgaaacta
     1201 cgggtgtaga tgtagcaaga gatagaattg tagagatttc gatactgaaa atctttccaa
     1261 acggaaacaa agaaagcaaa acatggttgg tcaatccaac aattcctatt cctattcatg
     1321 ccacactggt tcacggtatt tctgatgaaa aagtagccaa cgaacctaca tttgcggaac
     1381 tatcaagtca agtttacaac atgattaaag actctgactt ggctgggttt aattcagatc
     1441 gatttgacat accgctacta gcagaagaat tacttcgagc taacgttgat tttgaaatga
     1501 aaaatagagt ttcggtagat attcaaacta tttttcacaa aaaagaagaa cgaactcttt
     1561 ctgctgctta taaattctac tgcaatgaaa gtctagaaaa tgcacattcc gccgaagcag
     1621 ataccaatgc tacttatgaa atattaaaag cacaactaga ccgttacgaa gatttagaaa
     1681 acgacatgaa ttttctatct aagtttacaa caagaaaaaa atcagttgat tttgcgggtt
     1741 tcattgcctt aaacgaagat ggaaatgaaa tattcaactt tggaaagcac aaaggaaatc
     1801 ttgttgaaga tgtatttgat gctgaaccag gatattttgg ttggatacaa aatgccgatt
     1861 tcccattata tactaagaaa gtactaacag caatcaaact aagaaagtta agtacaaaac
     1921 aataacaatt tttagcgcat tagataatga aaattatttg tatcggaaga aattatgttg
     1981 accacattga ggaattgaat aacgaaagac cagccgaacc agttgttttt ttaaagcctg
     2041 atacagcaat attaccaaag aaaacacctt ttacgattcc cgaatttacc aacgaagtgc
     2101 atcatgaact tgaaataata gttaagatta ataaagtagg gaagtacatc aacaaaaaat
     2161 ttgctcacaa atattatgat gaaatcggtt tgggaatcga ttttactgct cgcgacatac
     2221 aaaatagcct caaagaaaaa ggattatctt gggaaaaatc caaagctttt gacggttcag
     2281 cgattattgg tgactttata ccgaaaaatt ctttagattc attagaaaac attaaatttg
     2341 aattaacaaa caatcaaaac atcgttcaag aaggaaatac taatttgatg atttggtcaa
     2401 ttgatgaatt gattgaacac gtttctcagt atttcacgct aaaaaaaggc gacattattt
     2461 ttactggaac cccaaaaggg gtagcaaaag ttgctccaaa tgatgttctt gaaggatttt
     2521 taaacaataa aaaattatta agattacaaa ttaaataatg gcaataaatt acaacctagc
     2581 aaaagtttac gaactttcag ataacgatcc tgaattcgta atgcaaatta tctctctatt
     2641 tgttacagaa gtaccagaag atatgaaata tatcgagcaa ggaattgaga taaaagatca
     2701 taaattggcc tacagctatg cacataaaat taaaccaacc cttgatttac ttggaatgaa
     2761 tcaagctttc gaagaaatac ttcaagtaga agcttggaca aaacgtgaag gaaaaagaaa
     2821 agaaattgat gcaacctatg acagtataaa agatcaagta gaaaaagcaa tcaaagaaat
     2881 aaaaaaagat ttcgaaattt aaagaaactt ttaaatacaa atcaaaagga actatcacac
     2941 aatagttcct ttttttatta attaaaacat taaaataaag tttgttttat agttcttaag
     3001 agaaaaggga ttcaacttta gagtatattt ttaattattt tttctatttt tataaaacca
     3061 aaccaaccac caaatggata aaacacaaaa aagttccttg ttcaaggtta tttccgcatc
     3121 ttcgatggga acgttaattg aatggtatga tttttacatt tttggcagtt tagccatcgt
     3181 aatttctacc aaattttttc caagcgaaaa tccaaccgcc gcttttttat ctacattagc
     3241 tacctttgcc gccggatttg tagtgcgacc atttggtgct ttgttcttcg gaagactagg
     3301 cgatttgatt ggtagaaaat atacgttcat ggctaccttg ctactaatgg gtggcgcaac
     3361 attcttaata ggatgtgtgc caagctttga aaccattggt tatgctgcgc caatattggt
     3421 gctaatcttg cgattattgc aagggttggc acttggcgga gaatatggtg gagccgccac
     3481 ttatgtagcc gagcacgctc caaaaggaca gcgcggttat tttacttcgt ggatacaaac
     3541 cactgcaacc gttggattat tcatttcctt gatggtgata ttgataacca aaaacgtttt
     3601 gtccgatagt caatttgacg cttggggttg gcgtgtgcct ttttgggttt cgatagtgat
     3661 ggtttttgtg tcctatatga ttcgaaaaaa aatgcacgaa tcgccagagt ttgccaaagc
     3721 caaagccgaa ggcaacatca gcaagaaccc actaaaagat agctttggaa ataaatacaa
     3781 tttaaagttt gtgttgcttg cgctctttgg tgcaacgatg ggacaaggcg tcatctggta
     3841 cactggacaa ttctatgcca tgagttttat gaaaatcgtg ctcgccatcg acagttcgca
     3901 agtagatacg ttgcttggaa ttgcactctt gtttggcaca ccattttttg tagtgtttgg
     3961 ttggttgagt gataaaattg ggcgaaagag tatcatgatg ttcgggatgt tgttggcaat
     4021 tttgctctat cgtcccatct ataaaacgat gtatgaaacg gtaaatgttg cacacaaaga
     4081 acttgtttct aaaaaattaa taactgaagg taacaatgta ataacctata aagtatatgc
     4141 agataaaaca gaagtaaaag aagttcaaaa ccaaaccgat ttgactataa aaactcaaat
     4201 gaccattagt aaccacgacc gttggatgtt agtctttcta gtcttcattc aagtgatttt
     4261 tgtaaccatg gtttacggac caatcgcggc ctttttggtc gaaatgtttc caactaaaat
     4321 tcgctatacg tcgatgtcgt taccatatca cgtgggcaac ggaatcttcg gaggattact
     4381 gcccgccatt tccacctatt ttgtaaccaa tgccaaaacc aacggcaatc cagagtatta
     4441 tctcgaaggt ttgtggtatc ctatcatcat tgcttcggtc tgctttgtca tcggaatgat
     4501 ttatttaagt tggaaaaatg aaactactaa cgattaattg aagcttatga acacaataaa
     4561 aagaatatta ggaatacttt ggttggtctt agctgttgct tcggcatact tttgtatttt
     4621 tgtttttgga ttgccaaagt tgacaaccgg aaaaacagaa gatatagtat ttggaggcat
     4681 aatcgttttc attttggcac ctctaattgt ttttggatta tctttatttg gctattattc
     4741 aatcaaagga gaatatgata attaattttt ttaaagtaac attcgatgag ctattataaa
     4801 atacacgatt tagagaatta cttcaaaatg tataaaaaat cggttcgcga accgaaaaag
     4861 ttttgggata aaattgccga cgaaaatttc tcgtggtacc aaaaatggga gaaagttttc
     4921 gattttgatt ttgagcaagc gaagttccgt tggtttattg atgccaaatt gaatattact
     4981 aaaaactgta tcgataggca tttggcacgt cgtggcgata aaacagctat catctttgaa
     5041 cctaacaatc cagaagaaga agcacaacac atcacttata ccgaattgta tgcaagagta
     5101 gccaaaatgg caaatgtgct aaccgatcaa ggaattaaaa aaggtgatcg agtttgcatt
     5161 tatcttccaa tgattccaga actagccgta tccattttgg cttgcgcacg aataggagcg
     5221 atacattccg taatctttgc tggtttctcg gcatcagccg tttccagtcg tattaatgat
     5281 tccgattgca aacttgtcat tacttccgat ggtggctatc gaggcgataa aaccatcgat
     5341 ttaaaaggta tcattgacga agctctacaa aaaacacccg gtgttgaacg agtattggtt
     5401 gtcaaaagaa ctcatgccaa catcacgatg aaagaaggaa gagatgtttg gttacaacct
     5461 ttattagata ctgctattcc taataatgta gcggcaataa tggatgccga agatccgttg
     5521 ttcattcttt atacatcagg ttctactgga aaaccaaaag gaatggtgca taccaccgca
     5581 ggttatatgg tttatacagc ttatactttt aaaaatatct tcaactacga agagaacgat
     5641 gtctattggt gtaccgccga tattggctgg ata
//
LOCUS       DAOOBH010000125         5649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_16485_length_5649_cov_5.439578, whole genome shotgun sequence.
ACCESSION   DAOOBH010000125 DAOOBH010000000
VERSION     DAOOBH010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16485_length_5649_cov_5.439578"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="PK218_10280"
     CDS             <1..1823
                     /locus_tag="PK218_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW98938.1"
                     /translation="LQKSTKVDLIKRGAYAWEPIINGMTTERTVVTIDGMRIFGACTD
                     KMDPVTSYVEVSNLSEATICSGQEGACFGTTIGGSIDLKRMKNTFRDTTWNFNLNTGF
                     ETNNAQKIAGLAIDYADSLFYVATDFMFRDADNYRAGNNKEVQFSQFRKLNLSQTIGY
                     KTSELSFLEASVIYDKATNVGYPALPMDVSLAQALITSLKYEYKPVDNLIKNWETKIY
                     FNTITHKMDDTKRPNVAIHMDMPGWSKTYGYYSKVEAQKGNHHFLVNLNSFLNNSLAE
                     MTMYPNNPNENPMFMLTWPDVDTFYNGIFLEDNYAFNCHSSLRVSATFGVHSNKIKSQ
                     FGLESLQIFYPEMKDTRTRFLKNVSTNYTITHGKFQCGVGAGYGERAPSVSEAYGFYL
                     FNSFDGFDQIGNPNLKNEKALEGNAFVSFKNEKWTLKAETYYFHIMDYIISRPNSSFV
                     PMTIGANGVKVYTALDYATIYNVSLSNDYRINDKINWNMKFIITKGIDSDKMNLPFMS
                     PFSYSTNVSYFDKKITADLTLLGNGIQNDFSPFYGEDKTPDFAVLNANFGYKFKWNRS
                     KLLTKIGVENILDTNYSTYLDWNNLPQKGRNFFVNINYML"
     gene            complement(1874..3571)
                     /gene="lysS"
                     /locus_tag="PK218_10285"
     CDS             complement(1874..3571)
                     /gene="lysS"
                     /locus_tag="PK218_10285"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPW98939.1"
                     /translation="MQLSEQEIIRREKLQALRDLTINPYPADLFPVNHTSKQVKENFS
                     EGKKVIVAGRLMSVRDQGKAAFAELQDSEGRIQLYFNRDTICPDDDKTLYNQVFKKLT
                     DLGDFIGVEGETFLTKVGAMCLRVEKFTFLSKTLRPLPLPKTDEEGNVHDAFVDPELR
                     YRMRYVDLVVNPQVKEVFIKRTKLFNAMRNFFNAAGYMEVETPILQAIPGGAAARPFI
                     THHNSLDIPLYMRIANELYLKRLIVGGFDGVYEFSKNFRNEGMDRTHNPEFTAMEIYV
                     AYKDYNWMMEFTENLLEYCAKEVNGTTETTFGQHTVNFKAPYARVTMTDAIKQFTGFD
                     ISGKTEAELFDAAKSMGIEVDETMGKGKLIDEIFGAKCEGNFIQPTFITDYPKEMSPL
                     CKEHRDNPELTERFELMVCGKEIANAYSELNDPIDQRERFENQMKLAEKGDDEANGII
                     DEDFLRALEFGMPPTSGLGIGMDRLMMFLTNNASIQEVLFFPQMKPEKKGPELTEDEK
                     HIIEILKANEGITIGDLKEKAAMSGKKWDAASKGISKHGLMKIVVDGDAKIVEYLGK"
     gene            3770..4474
                     /gene="lipB"
                     /locus_tag="PK218_10290"
     CDS             3770..4474
                     /gene="lipB"
                     /locus_tag="PK218_10290"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPW98940.1"
                     /translation="MNKKIILQELGTKDYKETWAYQEELFKEIVDVKIKNRREESNLP
                     TPNYFLFVEHPHVYTLGKSGDLSNLLLSEKQLEQKGASFYKINRGGDITYHGPGQIVG
                     YPILDLENFFTDIHKYLRFLEEVIIHTLAEYNIIGTRSEGETGVWIDVGTPFARKICA
                     MGVRASRWVTMHGFALNVNTDLGYFDNIIPCGIKGKAVTSLNNELGVEKMDREEVKQK
                     ILKHFQKLFEAEFKTS"
     gene            complement(4486..5145)
                     /locus_tag="PK218_10295"
     CDS             complement(4486..5145)
                     /locus_tag="PK218_10295"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPW98941.1"
                     /translation="MLLSNYSGLNIECGTDEAGRGCLAGPVTAAAVILPDSFEIDYLN
                     DSKQLSEKVREKLKPLLIKEAISFSVTHIYNVEIDEINILNASMKAMQESILKLNRTP
                     NYVIVDGNRSLNAKLGLKSTTGKNFSVDEIELLKSIPNKSIIKGDSKYLSIAAASVLA
                     KTFRDEYMNKIHEEFPMYNWKKNKGYPTKEHRDAIKKYGITKYHRKSFRLLPEQLLID
                     F"
     gene            5342..>5649
                     /locus_tag="PK218_10300"
     CDS             5342..>5649
                     /locus_tag="PK218_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPW98942.1"
                     /translation="MRTKFKWIFTLLLAFSMQFSFAQEKTVTGVVSDKTGPLPGANIV
                     VKGTTRSAQADFDGKFSIKAKAGEVLEVSFTGYNISTVTVGASNNYNVTLKEGVALEE
                     V"
BASE COUNT         1780 a          921 c          972 g         1976 t
ORIGIN      
        1 ttttgcaaaa gtctacaaaa gtagatttga ttaaaagagg tgcctatgca tgggaaccaa
       61 ttattaatgg catgactact gaacgaactg ttgtaacaat tgatggaatg cgaatttttg
      121 gtgcttgtac cgataaaatg gatccagtta catcctatgt agaggtttcc aatctttcgg
      181 aagctaccat ttgttcgggt caagaaggtg cttgttttgg tactactatt ggcggttcta
      241 ttgatttaaa aagaatgaaa aatacattta gagatactac ttggaatttc aatttaaata
      301 caggttttga aaccaataat gctcaaaaaa ttgctggttt agcaatagat tatgccgata
      361 gtttgtttta tgttgctaca gattttatgt ttcgcgatgc cgataattat agagcaggaa
      421 acaataaaga agtacaattt tcgcaattca gaaagttgaa tttgtcgcaa actattggtt
      481 ataaaacaag tgaattgagc tttttggaag cttcggttat ttatgataaa gctacaaatg
      541 ttggttaccc ggcattgccg atggatgttt ctttggcaca agcactaata acgtcactaa
      601 aatatgaata taaaccagta gataatttga ttaaaaattg ggaaactaaa atctatttca
      661 ataccattac gcataaaatg gatgatacca aaagaccgaa tgttgccatt cacatggata
      721 tgccaggttg gagtaaaacc tatgggtatt attctaaagt tgaagctcaa aaaggtaacc
      781 atcatttttt agtaaatcta aattcatttc tcaataattc attagcagaa atgacgatgt
      841 atccaaataa tccaaatgaa aacccaatgt ttatgctcac ttggcccgat gttgatactt
      901 tttataacgg catctttttg gaagataatt atgcgttcaa ttgtcactct tctttgcgtg
      961 tttctgctac ttttggggtt catagcaata agattaagag tcaatttgga ttagaaagtt
     1021 tgcagatttt ttacccagaa atgaaagata caagaacacg ttttcttaaa aatgtttcta
     1081 caaattatac tattactcac ggaaaattcc aatgtggagt tggcgctggt tatggcgaac
     1141 gagcaccatc tgtttcagaa gcctatggat tttatttgtt taatagtttt gatggttttg
     1201 accaaatcgg aaacccaaat ctaaaaaatg aaaaggcctt agagggaaat gcttttgtta
     1261 gttttaaaaa tgaaaaatgg acactgaaag ccgaaacata ctattttcat ataatggatt
     1321 atattatttc acgaccaaat agctcttttg ttccaatgac tattggtgcc aatggggtta
     1381 aggtttatac ggctttagat tatgctacaa tttacaatgt tagtctgtca aatgattatc
     1441 gaattaatga taaaatcaac tggaatatga aatttataat tactaaagga attgattcgg
     1501 ataaaatgaa tttgccgttc atgagtccgt ttagttattc taccaatgtg agttattttg
     1561 ataaaaaaat cacggcagat ttgacgttgc tgggcaatgg aattcaaaat gattttagtc
     1621 cgttttacgg tgaagataaa acgcctgatt ttgctgtttt aaatgctaat tttggctata
     1681 aattcaaatg gaatagaagt aagttattaa ccaaaatagg agtagaaaat attttggata
     1741 ctaattattc tacttatctt gattggaata atttgccgca aaaaggaaga aattttttcg
     1801 tgaatattaa ttatatgttg taattaagaa aggaactcat ttgagttcct tttttaatct
     1861 attttgagta atattatttt cctaaatatt caacaatttt cgcatctcca tcaaccacaa
     1921 ttttcatcaa tccgtgttta gatattcctt tgctggcggc atcccatttt ttaccactca
     1981 ttgctgcttt ttcttttaaa tctccaatag tgattccttc attggctttc aaaatttcga
     2041 taatgtgttt ttcgtcttct gttaattctg gtcctttttt ctctggtttc atctgaggaa
     2101 aaaacaacac ttcttgaatt gatgcattgt ttgttaaaaa catcatcaat cggtccatgc
     2161 ctattcctaa tccagatgtt ggaggcattc caaattctaa agcacgtaaa aaatcttcat
     2221 cgataattcc gtttgcttcg tcatcgcctt tttcggctaa tttcatttga ttttcaaagc
     2281 gttccctttg gtctattggg tcgtttaatt ctgaataagc gttggctatt tcttttccac
     2341 aaaccatcag ttcaaaacgc tctgtcaatt caggattatc tcggtgttct ttacacaacg
     2401 gcgacatttc ttttgggtaa tcggtaataa acgttggttg aatgaagttg ccttcgcact
     2461 ttgctccaaa tatctcatca atcaattttc ctttacccat ggtttcgtca acctcaattc
     2521 ccatagattt ggcagcgtca aaaagctcgg cttctgtttt acctgaaata tcaaaacctg
     2581 taaattgttt tattgcatcc gtcatggtaa ctcgtgcata tggtgcttta aagtttacgg
     2641 tatgttgccc aaaagtagtt tcggtagttc cattcacttc tttagcacaa tattcaagca
     2701 agttttcggt aaattccatc atccagttgt agtctttgta cgctacataa atttccattg
     2761 cagtaaactc tgggttgtgg gttctgtcca ttccttcgtt gcggaagttt ttagaaaact
     2821 catacacacc atcaaaaccg ccaacaataa gtctttttaa atacaattcg ttagcaatcc
     2881 tcatgtataa tggaatatcc aggctgttat gatgagtgat aaacggtctt gccgctgcac
     2941 ctccaggaat agcctgtaag attggtgttt caacttccat ataacctgct gcgttgaaaa
     3001 aattacgcat agcgttgaac aactttgttc ttttgatgaa aacttctttc acttgcggat
     3061 ttacaactaa gtcaacataa cgcattctat atcgcaattc tgggtcaaca aaagcatcgt
     3121 gcacattgcc ctcttcatcc gtttttggta acggtaatgg tcgtaatgtt ttgcttaaaa
     3181 aagtaaattt ttctacacgg aggcacattg cgccaacttt ggttagaaaa gtttcgcctt
     3241 caaccccaat aaaatctcct aaatcggtta attttttaaa aacttggttg tacaacgttt
     3301 tatcgtcatc aggacaaatg gtgtcacgat taaaatacaa ctgtattctt ccttcgctgt
     3361 cttgtaattc tgcgaaagcc gcttttcctt ggtcgcgaac actcattaaa cgcccagcaa
     3421 caataacctt cttaccttcg ctaaaattct ccttaacctg ttttgaagtg tgatttacag
     3481 gaaataaatc tgctggataa ggattgatgg ttaggtcacg caaagcctgt agcttttctc
     3541 gtctaatgat ttcttgttcc gaaagttgca tattcatgtg ttttaagtgt gcaaagatag
     3601 ttattagtag ccaagtttca aatacgccaa gattcaaagt ttttagtaat gtttactatg
     3661 ttttccatac actactcata cactatctaa gctttatcca tgtactattt atagtttatc
     3721 cctgaaggtt tcgtattttt gcaattcctt attttaattt tctattagca tgaacaaaaa
     3781 aataatcctt caagagctag gaactaaaga ttacaaagaa acttgggctt atcaagagga
     3841 gctttttaag gaaatagtag atgtaaaaat taaaaatagg agagaagaaa gtaatttacc
     3901 aacgccaaat tattttcttt ttgttgaaca tccacacgtt tatactttag ggaaaagtgg
     3961 cgatttgtct aatttacttt tatccgaaaa acagcttgaa caaaaaggtg cttcttttta
     4021 caagataaac cgtggtggcg atattaccta tcatggacca gggcaaattg ttggataccc
     4081 cattttagat ttagaaaatt ttttcacaga tattcataag tatcttcgct ttttagaaga
     4141 agtaattatc cacactttag cagagtacaa tataatagga acaagaagcg aaggtgaaac
     4201 tggtgtttgg attgatgtcg gaactccatt tgctagaaag atttgtgcca tgggtgttcg
     4261 tgcatctcga tgggttacaa tgcatggatt tgccttgaat gtgaatactg atttaggtta
     4321 ttttgataat attatacctt gtggtataaa aggaaaagca gttaccagtt taaataatga
     4381 acttggtgtt gaaaaaatgg atagggaaga agtaaaacaa aaaatcctaa aacattttca
     4441 aaaactattt gaagccgagt ttaaaacttc ttaaatcatc ttcaatcaaa aatctatcaa
     4501 aagttgctct ggtaataatc taaagctttt tctatggtat tttgttatac catatttttt
     4561 aatagcatct cggtgttctt tggttggata gcctttgttc tttttccaat tgtacatagg
     4621 gaattcttca tgtattttat tcatatattc atcgcgaaat gtttttgcta aaacagaagc
     4681 cgctgcaatg ctcaaatatt tactatcacc tttaataatg cttttattcg gtatcgactt
     4741 taagagctca atttcatcta cagaaaagtt tttgccagtt gttgatttta atccaagttt
     4801 ggcgtttaaa cttctgtttc catcaacaat cacataattt ggtgtacgat ttagttttaa
     4861 aatactttct tgcatggctt tcatagatgc gtttaaaata tttatttcat ctatttctac
     4921 attatatata tgtgtaaccg aaaaagaaat agcttcctta ataagtaacg gttttaattt
     4981 ttcgcggact ttttcagata attgtttaga gtcattgaga taatctattt caaaactatc
     5041 aggaagtata acagctgcag ctgtcactgg tccggccaga cagcctctgc cagcttcatc
     5101 agtgccacat tcgatgttta aaccagaata attggaaagt aacataattt ttttataaaa
     5161 atataaatta tacgcaacca attcaataga taatcgtctt tattttgaat gaatttgttt
     5221 tcttgaaaaa catacagttg caaagtttaa caaatttaat ttgtgttatt aaatggtaaa
     5281 atttgttttt ttaagaaata attaagaagt ttgcggaata actaactcaa ataaaattaa
     5341 tatgagaacg aagttcaaat ggatttttac gctattattg gcgttttcta tgcagttttc
     5401 ctttgcacaa gagaaaactg tgacaggagt cgtttcagat aaaactggac cgcttcctgg
     5461 agcaaatatt gttgttaaag gaacaactag aagtgcacaa gcagattttg atggtaaatt
     5521 tagtattaaa gctaaagccg gagaagtttt agaagtttca tttacaggtt acaatatttc
     5581 aacagtgacg gtaggagctt caaataatta taatgttacc cttaaggaag gtgttgcttt
     5641 ggaggaagt
//
LOCUS       DAOOBH010000126         5615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_16612_length_5615_cov_4.058993, whole genome shotgun sequence.
ACCESSION   DAOOBH010000126 DAOOBH010000000
VERSION     DAOOBH010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16612_length_5615_cov_4.058993"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1746
                     /locus_tag="PK218_10305"
     CDS             136..1746
                     /locus_tag="PK218_10305"
                     /inference="COORDINATES: protein motif:HMM:NF025627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HPW98943.1"
                     /translation="MKKNTLLYVLLVFNLFAKAQTIGLLYHDSGASDGYTLFSPLKNN
                     EVFLINNCGEKVNQWTFTELPGATCYLLANGNLLRAGKDHIEIRDWSNTIIWSYATTA
                     NGIPQHHDIEPLPNGNVLCLVSESYTLAQMTAMGRNPANTNASFKSEKIVELHPTGLN
                     TASIVWEWKFMDHIIQDFDDTKSNYGIVSDHPELLDINYEGNPVDYIHTNGLDYNADL
                     DQILVSARHLNEIYIIDHSTTTQEAMGHTGGNLNHGGDFIWRWGNPEVYGQGTSADRK
                     LFLQHDPKWVENDYLDQGKITVFNNGDATTNQNFTSVCLIAPEIVNNIYTKTNNKFNP
                     ATYDWSWNGSILGIEVHENKQSGTHSLPNGNIILAETSTGRVSEITKSGTLVWSYKNP
                     TGSIVNSNPTYYQQNSTISGSDNSFFRAEKYPANFIGFTGQNMTSTTGIIENENALSS
                     VCLTLDNPDFSNQNIFVLNPSSNNSIQFNTTIAADSVLIFDINGRQVYAQNEFNGNKL
                     DVALTPSLYFIQIQNGNLIKKLKVIVTK"
     gene            1765..2355
                     /locus_tag="PK218_10310"
     CDS             1765..2355
                     /locus_tag="PK218_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPW98944.1"
                     /translation="MKNYFFIALCLNTLITFGQKTKFSIEASYPLPIDNNFIGEYKGI
                     ADFGIKYKIKNLEQFNFGVSLNSAYYTFEATRFFPAFDENLKFNTSLYTIQPRFFCEM
                     NLKKVTKLHPYAGIGYTFLIFKTKFSSPEVTNPNSSDNQSGLNINLGANYDLTNRFYV
                     NVAYDYLKILELESNIPKSSYNTNASIVKLGVGFRI"
     gene            2361..2942
                     /locus_tag="PK218_10315"
     CDS             2361..2942
                     /locus_tag="PK218_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98945.1"
                     /translation="MKNYILLFIIIPFTTFSQSKSELQNAITKLHQANFLMEFEDIAQ
                     LTYPKVYEADGKSAFLEKLDTDFQNDDYRMRLQLEKVPFLFSEFKTIGAQTFCVVRSR
                     NPKRYTFEKKLTATKAEEKKTWLQEKEKTQNITFEPNRNSFNVRSESIYVAVFDAETM
                     GEWKFFNLDDAFQSNAFNTLFDNNIKITLGLNN"
     gene            complement(3136..4539)
                     /locus_tag="PK218_10320"
     CDS             complement(3136..4539)
                     /locus_tag="PK218_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPW98946.1"
                     /translation="MLLAKEILDKLTAIVGENYIFTDQETRNHYGHDETEDYVFPPSV
                     VVKPANTQEISEIIKLANDYKIPTTPIGARTGLSGGALSIYEGIGLSMERLNKIIEID
                     EQNLQVTVEPAVITQVLRETIAEKGLFYPVDPSSMGSCWIGGNVAENSGGARAVKYGV
                     TKDYVLNLEVVLPSGEIIWTGANTLKNSTGYNLTQLMVGSEGTLGVITKIVLKLIPKN
                     NHNVLMMVPFYKAHEACEAVSAIFRAGITPSALEFMERDAIDWTMKYVEGINITIKPE
                     HQAHLLIEVDGNYPDILMQEAEKILSVVEAFEIDEVLFADTEDQKNMLWKMRRAVGEA
                     VKSNSIYKEEDTVVPRYMLPELLKGIKEIGAKHGFKSVCYGHAGDGNLHVNIIKGNMT
                     DDNWNTQVPIGIREIFQLTVALKGTLSGEHGIGYVQKNYMNIAFSKANLELMESIKRV
                     FDPNNVLNPGKIFPDSN"
     gene            4607..>5615
                     /gene="meaB"
                     /locus_tag="PK218_10325"
     CDS             4607..>5615
                     /gene="meaB"
                     /locus_tag="PK218_10325"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPW98947.1"
                     /translation="MKKNNPKSALTENDGIPQPESISAFSLKKVIAKRKIQPSADDLI
                     SGILNHDKVALSRAITLVESTNPTHLEKANQIINGCLPHANNSIRIGITGVPGVGKST
                     FIEAFGTYLTSIGKKVAVLAVDPSSTISHGSILGDKTRMEELVKDENAFIRPSASGDT
                     LGGVARKTRETIILCEAAGFDTIIIETVGVGQSETAVHSMVDFFLLLKIAGAGDELQG
                     IKRGIMEMADTIVINKADGDNVAKAKLAKTEFNRALHLFPAKNSGWIPKVTTCSAFEK
                     TGIDGVWKIISEYFELVKTNDYFNAKRQSQNQYWMLETINEQLKNRFYTHPEIASLLE
                     TH"
BASE COUNT         1907 a         1022 c          941 g         1745 t
ORIGIN      
        1 gggttttagt ttctgaataa tatttaatta actataattt caatcctcaa tggttttact
       61 attgaggatt ttttaattaa aatatgttga agcaacattt ataaaaacat tttttttaaa
      121 tttgaaaaaa aagatatgaa aaaaaatacg ctattatatg tcttattagt attcaattta
      181 tttgcaaaag ctcaaactat tggcttgtta tatcatgata gcggagcttc tgatggttat
      241 acattatttt ctccactaaa aaacaacgaa gtatttctaa tcaataattg tggtgaaaag
      301 gtaaatcaat ggacatttac tgaacttcct ggagcaacat gttatttact tgcaaatgga
      361 aatttactac gagctggcaa agaccatata gaaataagag attggagtaa caccattatt
      421 tggagttatg ccacgacagc caatggaatt cctcaacatc acgatataga acctttaccc
      481 aatggaaatg ttttgtgtct agtgtctgaa tcctatactt tggcacaaat gactgccatg
      541 ggtagaaatc cagcaaatac aaatgccagc tttaaatctg aaaaaatagt tgaattgcat
      601 ccaacgggat taaacacagc ctcaatagtt tgggagtgga aatttatgga tcatataatt
      661 caagattttg atgacactaa gtctaactat ggtattgtaa gcgatcatcc cgaattactt
      721 gatattaatt atgaaggtaa tccagttgat tatattcaca ccaatggttt ggattacaat
      781 gccgatttag atcaaattct ggtttcagca cgacatttga atgaaattta tattattgat
      841 catagtacaa caacacaaga agctatggga catactggag gaaacttaaa tcatggtggc
      901 gattttattt ggcgttgggg aaatcccgaa gtgtatggac aaggaactag tgcagacaga
      961 aaacttttcc tccaacatga tccaaaatgg gtagaaaatg actatttaga ccaaggaaaa
     1021 attaccgtct ttaacaatgg tgatgctact accaatcaaa attttacttc ggtatgcttg
     1081 attgcaccag aaattgtaaa taacatctat accaaaacca acaataaatt caatcctgca
     1141 acttatgatt ggagttggaa tggttcaatt ttgggaatag aagtacatga aaataaacaa
     1201 tctggcacac attcgttacc caatggaaac atcatacttg ctgaaacttc tacaggaaga
     1261 gtatccgaaa taacaaaatc aggaactcta gtatggtctt acaaaaaccc tactggaagc
     1321 attgttaact caaatccaac ctattatcaa caaaacagta ctatttcagg aagtgacaat
     1381 tcttttttta gagcagaaaa ataccctgcc aattttattg gatttacagg tcaaaacatg
     1441 acatcaacaa caggaattat tgaaaatgaa aatgctttgt ctagtgtatg tcttacatta
     1501 gacaatcctg attttagtaa ccaaaacatt tttgtcttaa atccttcatc aaacaattct
     1561 attcaattta acactactat cgcggcagat tctgttctaa tttttgatat taatggaaga
     1621 caagtttatg cacaaaatga gttcaatgga aataaattag acgttgcttt aactccatca
     1681 ctatatttta tccaaattca aaatggaaat cttattaaaa aacttaaagt aatcgttact
     1741 aaataactaa atcattacta ttttatgaag aactattttt ttattgcact gtgcttgaac
     1801 actcttatta cttttggaca aaaaactaag tttagcatag aagctagtta tccattgcca
     1861 attgataata attttatcgg tgaatataag ggaattgcag attttggtat taaatataaa
     1921 atcaaaaatc tggagcaatt caattttggt gtttccttga atagtgctta ttatactttt
     1981 gaagctactc gttttttccc agcttttgat gaaaacttaa aatttaatac ctcattatac
     2041 acaatacaac caagattttt ttgtgaaatg aatttaaaaa aggtaacaaa acttcatcct
     2101 tatgcaggaa ttgggtacac ttttttaatt tttaaaacaa aattctcaag tcctgaagtt
     2161 actaatccaa atagtagtga taatcaaagt ggtttaaata taaatcttgg tgcgaattat
     2221 gatttgacaa accgttttta tgtaaatgtt gcttatgatt atctcaaaat ccttgagtta
     2281 gaaagtaaca ttcctaaaag tagttacaac acaaatgcaa gtatagtaaa acttggtgtt
     2341 ggatttagaa tttaaatagc atgaaaaact acattttact attcatcatt attcctttta
     2401 caacgttctc ccaatcaaaa tcggagttac aaaacgctat cacaaaattg catcaagcca
     2461 actttttaat ggagtttgaa gatatcgctc aattgacgta tcctaaagtg tatgaagctg
     2521 atggaaagtc tgcttttctt gaaaaattag ataccgattt tcaaaatgat gactaccgaa
     2581 tgcgtttaca actagaaaaa gttccttttc tatttagtga attcaaaacc ataggtgctc
     2641 aaaccttttg tgtagtcaga agtcgaaacc caaaacgata cacatttgaa aagaaattaa
     2701 ccgcaaccaa agccgaagaa aagaaaacgt ggttgcaaga aaaagagaaa acacaaaaca
     2761 taacttttga accaaataga aacagtttta atgtgcgaag cgaaagcatt tatgtggctg
     2821 tttttgatgc agaaacaatg ggtgaatgga agtttttcaa tctagatgat gcatttcaat
     2881 caaatgcttt taatacttta tttgataata atataaagat tactcttggt ttaaataatt
     2941 agtaggtatc tttactttaa ctaatcaata aacccaaata gttatggcaa aaggtaaaga
     3001 cattcaaaaa tcggctaaaa aagaaccgct taaaactgca aaagaaaaga aagaagaaaa
     3061 gcgaaataaa aagaatgctc ctaaaagaga ttaaaaaaat atccgtcaac attgacggat
     3121 attttttttg ttttattaat tactatccgg aaaaatcttt ccaggattta gcacattgtt
     3181 tgggtcaaaa acacgtttaa tgctttccat taattctaaa tttgctttag aaaaagcaat
     3241 gttcatataa ttcttttgca cataaccaat gccatgttcg cccgaaagtg ttccttttag
     3301 agcaacggtt agctgaaata tttctctaat tccaatagga acttgcgtat tccaattatc
     3361 atctgtcatg ttgcccttaa tgatgtttac gtgtagattt ccatcgcctg catgaccata
     3421 acacaccgat ttgaaaccat gcttcgctcc tatttctttg atgcctttca acaattcggg
     3481 caacatatat cgtggtacaa ccgtatcttc ttccttataa atagaattcg attttacggc
     3541 ttcacccact gcacggcgca ttttccaaag catgttcttc tggtcttcgg tgtcggcaaa
     3601 taatacctca tctatttcaa aagcttctac taccgaaaga attttttcgg cttcttgcat
     3661 taaaatatct ggataatttc catccacttc tatcaataaa tgcgcttgat gttctggctt
     3721 tatggtgatg tttataccct caacatactt catcgtccaa tcaatagcat cacgctccat
     3781 aaactccaaa gcacttggcg taattcctgc gcggaagatt gccgaaacag cttcgcatgc
     3841 ttcgtgtgct ttataaaaag gcaccatcat taatacatta tggttatttt ttggaattaa
     3901 tttgagaaca attttggtaa taacacccaa tgttccttcg ctgcctacca tcaactgggt
     3961 taaattatat ccggttgagt tttttaacgt atttgcaccc gtccaaatga tttcaccact
     4021 aggcaaaacg acttccaaat tgagtacata atctttggtt acaccatatt ttaccgctct
     4081 tgcaccacct gagttttcgg ctacattgcc accaatccaa caacttccca tactgcttgg
     4141 gtcaacagga tagaacaatc ctttttcggc aatggtttct cttagtactt gtgtgataac
     4201 ggctggttca acggtaactt gaagattttg ctcatcaatt tcgatgattt tattcaaacg
     4261 ctccattgac aacccaattc cttcataaat tgaaagagca ccaccactca agccggttcg
     4321 tgcacctatg ggtgtggtag gtattttata atcgttggct aatttgataa tttccgaaat
     4381 ctcttgggta ttggctggtt ttactacaac acttggcgga aaaacatagt cttccgtttc
     4441 gtcatgtcca tagtgattgc gggtttcttg gtccgtaaaa atatagtttt cgccaactat
     4501 agcggttaat ttatcgagaa tttctttggc taaaagcatg tgtctaattt tacacaaaaa
     4561 taaacattat ctacaaaaag ttgtattttt accaaaagca tttttcttga aaaaaaataa
     4621 cccaaaaagc gcacttacag aaaacgatgg cattccgcaa ccggaaagca tcagtgcctt
     4681 ttcattaaaa aaagtaattg caaagcgcaa aatacaacct agtgctgacg acctgatttc
     4741 gggcattcta aaccacgaca aagtagccct gtcgcgcgcc attactctag tagaaagcac
     4801 caacccaacg catttagaga aagccaatca aatcattaac ggctgtttgc cccatgccaa
     4861 taactctatt cgcattggta ttacgggcgt tccgggtgtg ggaaaatcta cgtttattga
     4921 agcctttggt acttacttga cttctattgg gaagaaagtg gccgtgctag ccgttgaccc
     4981 aagttcgacc atcagtcatg gtagtatttt gggcgataaa acccgtatgg aagaattggt
     5041 caaagacgaa aacgctttca tcagaccatc ggcatcggga gatacacttg gtggcgtggc
     5101 gcgaaaaacc agagaaacta ttattctctg tgaagcggca ggttttgata cgattattat
     5161 agaaacggtt ggcgttgggc aaagtgaaac tgccgtacac agtatggtcg atttctttct
     5221 actgcttaaa attgccggtg ctggcgatga actgcaaggc atcaaacgcg gtattatgga
     5281 aatggccgat actattgtta ttaataaagc cgatggcgat aacgtagcca aagccaagct
     5341 agccaaaacc gaatttaacc gagcgttgca tttgtttcct gcaaaaaaca gcggttggat
     5401 acccaaagta actacctgta gtgcttttga aaagacaggc attgatggtg tttggaagat
     5461 tatttcagag tattttgaac tggtaaaaac caacgattac tttaatgcca aacgccaaag
     5521 tcaaaaccaa tactggatgc tcgaaaccat caacgagcaa ctgaaaaacc gcttctacac
     5581 gcatcctgaa attgcatcgc tactagaaac ccaca
//
LOCUS       DAOOBH010000127         5310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_17787_length_5310_cov_5.417507, whole genome shotgun sequence.
ACCESSION   DAOOBH010000127 DAOOBH010000000
VERSION     DAOOBH010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17787_length_5310_cov_5.417507"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PK218_10330"
     CDS             complement(<1..823)
                     /locus_tag="PK218_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HPW98948.1"
                     /translation="MVKAKVKKPSFRRKNAIKPKAKFPLGKSILVLVLILFSIMAYHY
                     RDAISFYINSKRNSGTTIDKVAQARIHQVLTKHEDKTFGFDVSEYQGEINWKEIDSVE
                     HKFPLHFVFIRATAGKDKVDSRFKTNWNSTKKHHLIRGAYHYYRPNENSIAQADNFIR
                     TVSLQKGDLPPILDIEQMPESQSIAKLKIGLKRWLDRVESHYKIKPIIYSGERYYSDF
                     LRDEFSEYSFWIANYNFFEENIKDDWLFWQFTEKAVVEGIYENVDLNIFNGTPIML"
     gene            complement(834..1547)
                     /gene="pssA"
                     /locus_tag="PK218_10335"
     CDS             complement(834..1547)
                     /gene="pssA"
                     /locus_tag="PK218_10335"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPW98949.1"
                     /translation="MNIKAQIPNMFTMLNLFSGCVALVFVAEFNFELAFYFVCLGIFF
                     DFFDGFFARKFDVAGPLGVQLDSLADMVTSGVVPGFVMFQMIKKYNSIGFEDYLPFLG
                     FIITLGACYRLAKFNIDTRQSDSFIGLPTPANALFITSLPLVFKNYTNNSIVELLHNE
                     WFLLMITFISAYIMNAEIPLFSLKIKNFSVAKYKFQLFFLLFSVLLLVFLQILAVPLI
                     IISYVLLSVVNNLISKESK"
     gene            1744..2727
                     /locus_tag="PK218_10340"
     CDS             1744..2727
                     /locus_tag="PK218_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPW98950.1"
                     /translation="MNIGVDAAALGMSNAVTASTNNVNSGYWNPAGLLSIEDSEASLM
                     HASYFANIAQYDYAAYAKKIDDRSAWGISLIRFGVDDILNTTQLIDSQGNIDYNRISL
                     FSTADYGMTFSYARKSQLKEIQYGINAKVIRRIIGDFASSWGFGIDAGIKFERDSWKF
                     GVMLRDITTTYNIWNIDEEKYNQIKDAVAGQNQEAPESTEITAPKAQLGIAKLFEFHY
                     DYTLLAAVNLNMQFARTNDVFASEAVSIDPAVGFEFGYTDLVFLRAGVGNFQNVEQID
                     GSKKINFQPNIGLGFKYKGIQIDYALTDLGDQSAALYSNIFSVKVDLSIFR"
     gene            2735..3865
                     /locus_tag="PK218_10345"
     CDS             2735..3865
                     /locus_tag="PK218_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HPW98951.1"
                     /translation="MKKKIFLLFLFLCSAFSFSQNPQLSENTQISILTCGRGEELYST
                     FGHTALRIKDETNQLDIVYNYGMFDFRTENFYLKFVKGDLQYFMNVTSFEDFILEYQM
                     DKREVVEQTLDLSITKKQELFNLLNNNLFSTEKYYTYKFIDRNCTTMVANKLNEIYKE
                     IHFEKVDDKTISYRKLLYPYFDNYFWYKLGINIVFGLKTDTDAEKLFLPKELLNSIDK
                     AKINGKLIVLSKNTIVKGETVQPKFSFLNSIYVIIISLLILILLNSKILFKTYLFING
                     LLGLLLCLIGFYSQHQEVLWNYNALLFNPLFILIPFINRNLSRKITLTSLLLLLIYCM
                     AMLTKPHLIIMLPFIITNLYILIKLNGRNPIQLLTSIKKNRA"
     gene            complement(3833..5221)
                     /locus_tag="PK218_10350"
     CDS             complement(3833..5221)
                     /locus_tag="PK218_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HPW98952.1"
                     /translation="MKKKEIHFEISERKILLRLFDVLSVFLSLYFVSLIFKFDYFNIS
                     TTNFYWTIVLGIYLLFIGSVFEMYNLQVASNQFQIIKSSILTSSTTVLLYLLTPIYTP
                     VLPSNRIQILIFFLAIFGALFIWRMIYVRFFASNRFVKKVILVCDQDQVQELVNGLES
                     VDPHYKVLGFVNSDSNSDRNDTILIKNIPISNLRNFVSKNSISEIVVASQKTDGITVE
                     LYNQLITILENGFIIREYTQVYESITQRIPVQYVSRDFYRYFPFSRSNQNHLYLLVVR
                     FLEILLSLIGLAVGIALIPFVIVCNAFWNKGKLFYKQERVGKNGKVFNIVKFRTMVKN
                     AEQQGAVFATSNDSRITPFGKILRKSRIDEFPQFINIFKGEMAVIGPRPERPIFVKEI
                     AERMPFYETRHVIKPGLTGWAQVNYSYGETIDDSLIKLQYDLYYIKHRSLFLDFNIIF
                     KTLSTVLFYRGQ"
BASE COUNT         1908 a          802 c          758 g         1842 t
ORIGIN      
        1 tgagcattat tggtgttcca ttgaaaatat tcaaatccac attttcataa atcccttcca
       61 caactgcttt ctctgtaaac tgccaaaaca accaatcgtc tttaatgttt tcttcaaaaa
      121 aattataatt ggcaatccaa aaagaatatt ctgaaaattc atctctcaaa aaatcagaat
      181 aataacgctc accagaataa attataggct taattttata atgtgattct actctatcca
      241 accaacgctt taaaccaatt tttaattttg caatagattg actttcgggc atttgctcaa
      301 tatcaagaat tggtggtaaa tcgccttttt gaagcgaaac ggttctgata aaattatcag
      361 cttgtgcaat cgaattctca tttggacgat aataatgata agcaccacga attaaatgat
      421 gtttcttggt agaattccag ttggttttaa accgagaatc tactttgtct tttccagctg
      481 tagccctaat aaacacaaaa tgtagcggaa acttatgttc cactgaatca atttctttcc
      541 agtttatttc tccttggtat tcggatacat caaagccaaa agttttatcc tcatgttttg
      601 tcaatacctg atgaattctg gcttgagcaa ctttatcaat ggttgttcca gagtttcttt
      661 ttgaatttat ataaaaagaa atagcatctc gataatggta agccattata gaaaataaaa
      721 tcaaaacgag tactaaaatt gattttccta aaggaaattt tgctttaggt ttgattgcat
      781 ttttacgtct aaaagatggc tttttgactt tagcttttac cattgaatta tttctattta
      841 ctttctttag aaattaaatt attcacaact gataataata cgtaactaat aattatcaac
      901 ggaacagcta gaatttgtaa aaacactagt aacaaaacag aaaacaacaa aaagaaaagt
      961 tggaatttat atttagcaac actaaagttt tttattttta aagagaataa tgggatttca
     1021 gcattcataa tatatgcact aataaaagtt atcatcaata aaaaccattc gttatgcaat
     1081 aattccacaa tagaattatt tgtatagttt ttaaaaacca acggtaaact agtaataaac
     1141 aatgcatttg caggcgttgg caaacctata aaagaatcag actgtcgagt atcaatattg
     1201 aattttgcca atcggtaaca agcaccaagt gtaatgataa atcctaaaaa aggtaaataa
     1261 tcttcaaaac ctatggaatt gtattttttt atcatttgaa acataacaaa acctggaaca
     1321 acaccacttg ttaccatatc tgcaagtgaa tccagttgaa cgcctaatgg accagctaca
     1381 tcgaatttac gagcaaaaaa accatcaaaa aaatcaaaga aaatacctaa acaaacaaag
     1441 taaaatgcta attcgaagtt aaattctgct acaaaaacca aagcaacgca accactaaac
     1501 aaattaagca tcgtaaacat attgggaatc tgcgctttga tgttcatatt ttattggtgt
     1561 ttatttttaa aatttttaca aatttaatac aatatgttta ccctttctgc gttttatatt
     1621 agagattttt atatgatatt tgtaaaaaga aaaattgcat tgaaaaaaag acttccatta
     1681 ttgctattgt tttttagcgt attaactcat gctcagtcta ttagaaagta ttctaatgaa
     1741 tttatgaata taggagttga tgctgctgcg cttggaatgt caaatgcagt tacagcttct
     1801 actaataatg taaattcagg atattggaat ccagcgggtt tactttccat tgaagattcg
     1861 gaagcatcgt tgatgcacgc aagttatttt gccaatattg cacaatatga ctatgctgct
     1921 tatgctaaga aaattgacga tagaagtgct tggggaattt ctctaattcg ttttggtgta
     1981 gatgatattt taaacactac ccaacttata gacagccagg gtaatattga ttacaacaga
     2041 atcagccttt tttcaacggc cgattatgga atgacttttt cttatgccag aaaatcacaa
     2101 ttaaaagaaa ttcaatatgg tataaatgct aaagttattc gaagaataat tggagatttt
     2161 gccagttctt ggggatttgg aattgatgct ggaattaaat ttgaacgcga tagttggaag
     2221 tttggtgtga tgcttcgcga tataacgacg acctacaata tttggaatat tgatgaagag
     2281 aaatacaatc aaatcaaaga tgcagtagca ggtcaaaatc aggaagctcc cgaaagtact
     2341 gaaattacag ctccaaaagc acaacttggt attgcgaaat tatttgaatt tcattatgat
     2401 tatactcttt tggccgcggt aaatcttaac atgcaatttg cccgaaccaa tgatgttttc
     2461 gcttcagaag cagttagtat tgatccagcc gttggttttg aatttggtta cacggatttg
     2521 gtttttcttc gtgctggagt tggaaatttt caaaacgtgg aacaaattga tggttctaaa
     2581 aaaataaatt ttcaacctaa cattggatta ggttttaaat ataaaggcat tcaaattgat
     2641 tatgccttaa ccgatttagg cgaccaaagt gctgctttat attctaatat cttttcagta
     2701 aaagtagatt taagtatttt tagataactt cattatgaaa aaaaaaatat ttcttttatt
     2761 tctattttta tgtagcgcat tttctttttc gcaaaatcct caactttctg aaaatactca
     2821 aatcagcatt ttaacatgtg gtcgtggaga agaattatat tcaacgtttg gtcatactgc
     2881 attgcgaatt aaagacgaaa caaatcaatt ggatattgtt tacaactacg gaatgtttga
     2941 ttttaggact gaaaattttt atttaaaatt tgtaaaaggt gatttacaat actttatgaa
     3001 cgttacatcg tttgaagatt ttatccttga atatcaaatg gataaaagag aagttgttga
     3061 acaaacatta gatttatcga ttacaaaaaa acaagaactt ttcaatctat taaataataa
     3121 tttattttca accgaaaaat attataccta caaatttatt gatagaaact gtactacgat
     3181 ggttgccaat aaattaaatg aaatttataa agaaatccat tttgaaaaag tagatgacaa
     3241 aactattagc tatcgtaaac ttttgtaccc ctattttgat aattattttt ggtataaact
     3301 agggattaat atcgtttttg ggttgaaaac agacaccgac gctgagaaac tttttttacc
     3361 aaaagaactt ttaaacagta ttgataaagc taaaattaat ggaaaattga tagttttatc
     3421 taaaaacact atcgttaagg gagaaaccgt tcaaccaaaa ttttcttttt tgaatagtat
     3481 ctatgttatt attataagtt tattaatttt aatattacta aactctaaaa ttctatttaa
     3541 gacttaccta tttataaatg gtttattggg attacttctt tgtttaattg gtttctattc
     3601 gcaacatcaa gaagtattat ggaattacaa tgccttgtta tttaaccctt tattcatttt
     3661 aatacctttc attaatagaa atttatcgag aaaaatcaca ttaacttctt tattattgtt
     3721 gcttatttat tgcatggcaa tgcttactaa gccacattta ataattatgt tgccttttat
     3781 tattacaaac ttatatattt tgataaaatt gaacggaaga aaccctattc aattattgac
     3841 ctctataaaa aagaaccgtg cttaaggttt taaagataat gttgaaatct agaaataaac
     3901 ttcggtgttt gatataatat aaatcatatt gaagttttat caaactatca tcaatggtct
     3961 caccataaga ataatttact tgtgcccatc cagttaaccc tggttttatt acatgtctag
     4021 tttcataaaa aggcattctt tctgcaattt ctttcacaaa tattggtcgt tcaggtcgag
     4081 gtccaatgac tgccatttcg cctttaaaga tattgataaa ttgtggaaat tcatcaattc
     4141 ttgattttct aagtattttt ccaaatggtg taattctcga gtcattggaa gttgcaaaaa
     4201 cagcaccttg ttgttctgcg tttttaacca tcgttctaaa ttttacaata ttgaacacct
     4261 ttccattttt tccgacacgc tcttgcttgt aaaaaagttt tcctttattc caaaaagcat
     4321 tacaaacaat aacgaaaggt attaaagcta tcccaactgc caatcctatt agtgatagca
     4381 gtatttctaa gaacctaaca accaataagt aaaggtgatt ttgattgctt ctgctaaatg
     4441 gaaaatacct atagaaatct ctagaaacat attgcacagg aattcgttgc gtaatacttt
     4501 cataaacttg agtatattcc ctaattataa atccattttc taaaattgta atcaattgat
     4561 tgtaaagttc aactgttatg ccgtctgttt tttgagaagc aactacaatt tctgaaattg
     4621 aatttttact aacaaagttt ctaagattac tgattggaat attttttatc aaaatagtgt
     4681 catttctatc ggaattacta tcagaattta caaatcctaa aactttataa tgaggatcaa
     4741 cactttctaa tccgtttacc aattcttgta cttggtcttg atcacaaacc aaaattactt
     4801 ttttaacaaa acgattggag gcaaaaaaac gaacataaat cattcgccaa ataaaaagtg
     4861 cgccaaatat agctaagaaa aaaattaaaa tctgaatacg atttgatggt agaacaggtg
     4921 tataaattgg tgttaataaa tacaataaaa ccgtggtaga acttgtgaga atgctacttt
     4981 taataatttg aaattgatta ctggctactt gtagattata catttcaaaa acagatccaa
     5041 taaaaagtag gtatattcca agaacaatcg tccaatagaa atttgtagtt gaaatgttga
     5101 aataatcaaa tttaaaaatt aagctaacga agtacaaaga caaaaagaca gaaagcacat
     5161 caaacaatct taataatatt tttcgttcag aaatttcaaa atgtatctct ttttttttca
     5221 tattaaaaat attattcgca agttagcaaa tattgttatt tattctaata attccatcca
     5281 atccttttta attatttccc aatcaaaggt
//
LOCUS       DAOOBH010000128         5287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_17877_length_5287_cov_5.127102, whole genome shotgun sequence.
ACCESSION   DAOOBH010000128 DAOOBH010000000
VERSION     DAOOBH010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17877_length_5287_cov_5.127102"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1148
                     /gene="rocD"
                     /locus_tag="PK218_10355"
                     /pseudo
     CDS             51..1148
                     /gene="rocD"
                     /locus_tag="PK218_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
     gene            1188..1655
                     /locus_tag="PK218_10360"
     CDS             1188..1655
                     /locus_tag="PK218_10360"
                     /inference="COORDINATES: protein motif:HMM:NF036614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HPW98953.1"
                     /translation="MDELNKLELNELAIDSLRVSAKWSTFLSIIGFIGIGFMLIAALF
                     IGTAFSAMSGQSELQGMQTMNPMAGVLKFMPLLYVLIAVIYFFPVYYLFKYASGMKKA
                     LNFRNSEMVAESLVYLKSHHKFLGIMTIVMISLYIVIIIGAMIFAATMASSMM"
     gene            1728..1925
                     /locus_tag="PK218_10365"
     CDS             1728..1925
                     /locus_tag="PK218_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98954.1"
                     /translation="MKKLALPFIIVALIIGTYEYTKEKPNIFIVCLVIILFMVGMFQL
                     NVKIPSKFSDEDQENEIKDND"
     gene            1918..2454
                     /locus_tag="PK218_10370"
     CDS             1918..2454
                     /locus_tag="PK218_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPW98955.1"
                     /translation="MIKIGDNVSVLDDAIDGVVKRITGKTITVETTDGFDLNFQENEL
                     IIIGSNKLNISFNKSEIVNQKEIAKPNYVNREKKSKKEIPFPDFDLHIEKLVKNYKSL
                     SNHEILTIQSETAKRHIDFAIRNRIPKIVLIHGVGEGILKSELDFLLGRYEGVTFREA
                     NYQKYGQGATEIYFKQNK"
     gene            complement(2537..2881)
                     /locus_tag="PK218_10375"
     CDS             complement(2537..2881)
                     /locus_tag="PK218_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98956.1"
                     /translation="MKTSNTTTQKNSNIKMFVLFVVLFLSSSTMFAQETKETVSIVET
                     TTIVSTENTVASTSTEFAIWFVGSTTSTPEAKTSTLSFGKKHLINSGIPTNKILLKKV
                     LKTIVGQESNIA"
     gene            3182..3514
                     /locus_tag="PK218_10380"
     CDS             3182..3514
                     /locus_tag="PK218_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPW98957.1"
                     /translation="MKVEQKGNNTIIKNTQYKTAEFLSKLSQEYTTFKDKNLIVDISF
                     DAGLKLVDVKSFSDLIKKHKKNKKSFVLVANDFDFNTVPASITLVPSILEAHDIIEME
                     EIERDLGF"
     gene            3533..4444
                     /locus_tag="PK218_10385"
     CDS             3533..4444
                     /locus_tag="PK218_10385"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPW98958.1"
                     /translation="MKLTILGCYAATPRTITNPTSQVLEIRNRMFLIDCGEGTQVQLR
                     KNKIKFSKINHIFISHLHGDHFYGLVGLISTFMLLNRQTDLHVYGPKGIKEIILLQLK
                     YSNSYTGYNLYFHELDSKESEVVYEDDKVLVKTIPLKHRIYTNGYLFEEKPRERKLNI
                     AAVEELEIDRCYYQNIKNGKDFLLESGKLIPNDQLTFDAVAPMKYAFCSDTMYDESII
                     SLIHEVDVLYHETTFLEAEADKAEKTMHSTAIQAATIAKKANVKNLILGHYSTRYSSI
                     EFFKNEAETVFSKVLLADDGKSFDFEN"
     gene            4457..5101
                     /gene="pdxH"
                     /locus_tag="PK218_10390"
     CDS             4457..5101
                     /gene="pdxH"
                     /locus_tag="PK218_10390"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HPW98959.1"
                     /translation="MKDLSNYRKSYEKSELLETNIPEDPINLFHKWFYETEEFGNIEE
                     VNAMTVATIGLDGFPKSRVVLLKKFNEEGFIFYTNYSSEKGKAILNNPNICLSFFWHS
                     MERQVIIKGMAEKTSEIISDNYFQSRPDGSKLGAIVSSQSDVIPSRNYLDEKLKQLEK
                     QFDGKEIPRPDFWGGFLVRPVEVEFWQGRANRLHDRIRYKIQEDFTWKIERLSP"
BASE COUNT         1997 a          719 c          879 g         1692 t
ORIGIN      
        1 ggcaatgacc ttgattcaca gctgaataag ccgataaaaa atcataatat tttttatcgg
       61 cttattcagc tgtgaatcaa ggtcattgcc acccgaaaat tgttaatgca atgattaacc
      121 aagcgcaaac tttgacactt acatcaagag ctttttataa cgacaaactt ggagtttatg
      181 aagaatttgt aactaattat tttggttttg ataaagtatt gcccatgaat actggagcag
      241 aagctgtaga aactgccata aagatttgca gaaaatgggc ctacgagaaa aaaggaattg
      301 cagaaaacga agcacaaatt atagtttgtg atggtaattt tcatggaaga actaccacaa
      361 taatttcgtt ctcaaatgat gaaaatgcca gaaagaattt tggaccatac acttcaggat
      421 tcataaaaat atcttatgat gatattgatg ctttagaaaa aacaattaaa tcgtcaaaaa
      481 atattgcagg atttctagta gaacccattc aaggtgaggc aggagtttat gtaccaagtg
      541 aaggctattt aaccaaagca aaggctttgt gtgaagctaa caatgtactg tttattgctg
      601 atgaagtaca aactggaata gcaagaacag gaaaattatt agcagttaat catgaaaatg
      661 tgcagcctga tattttaata ctaggaaaag caatttctgg tggtgcttat ccagtttcag
      721 cggttttggc aaacgatgct attatgaatg tgatcaaacc aggacaacat ggttctactt
      781 ttggcggaaa cccagttgca gctgcagttg ccatcgcagc attagaagtg gtaagagatg
      841 agaagctttc ggaaaatgca gaacatttgg gaaaattgtt tcgttcagaa attgctaact
      901 atatcgaaaa atctacaata gcaactctag ttcgtggaaa aggtttacta aatgcaatag
      961 tgattaatga tactgaggaa agtgataccg catggaatat ttgtatagca ttgcgagaca
     1021 atggtttatt ggcaaaacct actcacggaa acattattcg ttttgcgcca ccattagtaa
     1081 tgaatgaaga acaattatta gattgtgttt ctattattat caatacattg aagcaatttg
     1141 aaaagtaatc aattaacctt tttataacca aacaaaaacc aaattttatg gatgaactaa
     1201 acaaattaga attgaatgaa ttagctattg attcattaag agtaagtgca aaatggagta
     1261 cttttttatc cataattgga tttataggta ttggatttat gcttattgca gctctattca
     1321 ttggcacagc ttttagtgca atgtctggtc aatcggaact acaaggcatg caaacaatga
     1381 atccaatggc aggtgtactg aaatttatgc ctttgcttta tgttttaatt gcagtaattt
     1441 acttttttcc agtatattat ttattcaaat atgcttctgg aatgaaaaaa gcattgaatt
     1501 ttagaaacag cgagatggta gccgaatcat tggtatattt aaaatcacat cacaaatttt
     1561 taggaattat gaccattgta atgatttcat tatacatagt tataattatt ggtgcaatga
     1621 tttttgcggc aactatggct tcaagcatga tgtaatacta taaaattaag ttcaaattaa
     1681 accagctttt tcagctggtt tttttatttt tgttaaaatt aaattccatg aaaaaattag
     1741 cgttaccttt tattattgtt gccttaatta ttggtacata tgaatacaca aaagagaaac
     1801 caaatatttt tatagtttgt ttagtaatca tactattcat ggttggaatg tttcagttga
     1861 atgtcaaaat tccaagtaaa tttagtgacg aagaccaaga aaacgaaatt aaagacaatg
     1921 attaaaatag gtgataacgt ttcagttctc gatgacgcca tagatggtgt tgtaaaaaga
     1981 attactggta aaacaattac ggttgaaaca acagatggat ttgatttaaa ttttcaagaa
     2041 aacgaattaa tcataatagg aagcaataaa ttgaacatca gttttaataa aagtgaaatt
     2101 gttaatcaaa aagaaatagc aaaaccaaac tatgtaaatc gagaaaagaa atctaaaaaa
     2161 gaaatacctt ttccagattt tgatttgcat attgaaaaac ttgtaaaaaa ctataaatca
     2221 ttatctaatc acgaaatctt aaccattcag tcggaaacgg caaaacgaca tattgacttt
     2281 gctatcagaa atagaattcc aaaaattgta ttgattcatg gtgtaggtga aggcattcta
     2341 aaatccgaac ttgattttct tttaggtaga tatgaaggtg ttacctttcg agaagctaat
     2401 tatcaaaaat acggacaagg tgcaactgaa atctatttta aacaaaataa ataaacaaaa
     2461 aagtctcgat aaatcaagac tttttaactt ttaactaaaa ctaaccaatt tatttttaaa
     2521 aaaaactaaa aatttattaa gcgatattac tttcttgtcc aacgatagtt tttaaaactt
     2581 ttttaagtaa aattttgttt gttggaattc cagagttaat taaatgtttt ttaccgaaag
     2641 ataaagttga agttttagct tcaggagttg aagttgtact tccaacaaac caaattgcaa
     2701 attcagtaga agtagaagca actgtatttt cggtagaaac aattgttgta gtttcaacga
     2761 tagaaactgt ttctttagtt tcttgagcaa acattgtaga agatgataaa aacaatacta
     2821 caaataaaac aaacatttta atgttagaat ttttttgagt tgtagtgtta gatgttttca
     2881 taatttctgt gtttttcgtt ttaattacat gtcaaagata ggttgacacc tttctgaagc
     2941 acaatttttt cgacaactaa cccagattgt cgtttaatcg acaaaaccaa tgacatcaag
     3001 ggttttcagc gtattaaatt tgcacttcat ctataaaatg ttaaaaaaac gtgtatttca
     3061 tcgatgaaat tccaaaaaat tctatgaact acatccttaa aaaggaaaac agaatcagaa
     3121 taaccgaaaa ctcgtaaaat cttagtaaat tagcatacta ttataagtaa agattaagaa
     3181 aatgaaagtg gaacaaaaag gaaacaacac gattattaaa aatacgcaat ataaaacagc
     3241 tgagtttttg tctaaactat cgcaggaata cactactttt aaagataaaa acctgatcgt
     3301 tgatatttcg tttgatgctg gtttaaaact tgttgatgta aagtcttttt cagatttaat
     3361 taaaaaacat aaaaaaaaca aaaaatcatt cgttctggtt gcaaatgatt ttgattttaa
     3421 taccgttcca gctagtatta ctttagttcc ttctatactc gaagcacatg atatcatcga
     3481 aatggaagaa atagaaagag atttaggctt ttaaaaacaa tccaatcaat taatgaaatt
     3541 aaccattttg ggctgttatg ccgctacacc aagaacgata accaatccaa cttcacaagt
     3601 tttagaaatt agaaatcgaa tgtttctgat agactgtgga gagggaacac aggtgcaact
     3661 tcgaaaaaat aaaattaaat tttccaaaat caatcatatt tttatttctc atttgcatgg
     3721 cgatcatttt tatggattgg taggattgat ttcaaccttt atgttgttga accggcaaac
     3781 ggatttacat gtgtatggtc caaaaggaat taaagagatt atactactcc agttaaaata
     3841 ttccaattca tacacaggct ataatttata ttttcacgaa ttagattcaa aagaaagtga
     3901 agtagtttat gaagacgata aagtactcgt taaaacaatt ccattaaaac acaggattta
     3961 taccaatggc tatctttttg aagaaaagcc aagagaacga aaattaaaca ttgctgcagt
     4021 agaagaactc gaaatagaca gatgctatta tcagaatatt aaaaacggaa aagatttttt
     4081 gttagaaagt ggaaaattaa ttccaaatga ccaattaact tttgacgccg ttgctccaat
     4141 gaaatatgct ttttgtagcg atactatgta tgatgaatcg ataatttcgt taatacatga
     4201 agttgatgtt ttgtatcatg aaactacatt tttagaagca gaagctgata aagcagagaa
     4261 aacaatgcat agtacagcga ttcaagcggc aaccattgct aaaaaagcta atgtgaaaaa
     4321 tttaattctt ggacattata gtactagata ttcatcgatt gaatttttta agaatgaagc
     4381 cgaaactgtt ttctcaaaag ttttattagc cgacgatgga aaatcatttg attttgaaaa
     4441 ttaattccca atagaaatga aagatttaag caattataga aaatcttatg aaaaaagtga
     4501 acttttggaa accaatattc ccgaagatcc aatcaattta tttcacaaat ggttttacga
     4561 aactgaagag tttggaaata ttgaagaagt aaatgccatg acagtagcaa caattggatt
     4621 agatggtttt ccaaaaagta gagtagtact tttgaaaaaa tttaacgaag aaggatttat
     4681 tttctacacc aattacagtt cagaaaaagg aaaagccatt ttaaacaatc cgaatatatg
     4741 tttatcattc ttttggcaca gcatggaaag acaagttatt ataaaaggaa tggcagagaa
     4801 aacttctgaa atcatatcgg ataattattt tcaatcaaga ccagatggaa gtaagcttgg
     4861 cgcaatagtt tcatcgcaaa gcgacgtgat tccgtctcga aattatttgg atgaaaagtt
     4921 aaaacaattg gaaaagcaat ttgacggaaa agaaattcca cgacctgatt tttggggagg
     4981 ttttttagta agaccagttg aagttgagtt ttggcaagga agagccaata gactacatga
     5041 tagaattcgt tacaaaattc aggaggattt tacttggaaa attgaacgat tgtcaccatg
     5101 aatttactat tcaattacta taaaaaccac ttttaagtgg ttttttttat tttaatattt
     5161 aaattgtcac tatttaaaaa atattctttt gtaagattaa tttattaata aaataaataa
     5221 ttatgcattt catcgatttt atttgttatt taaacgatga ttgatgtatt attgctgaac
     5281 caaatca
//
LOCUS       DAOOBH010000129         5221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_18164_length_5221_cov_6.372822, whole genome shotgun sequence.
ACCESSION   DAOOBH010000129 DAOOBH010000000
VERSION     DAOOBH010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18164_length_5221_cov_6.372822"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PK218_10395"
     CDS             <1..161
                     /locus_tag="PK218_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPW98960.1"
                     /translation="FQEKGIFDKEVATKFKDNILSKGGTELPMELYKKFRGKEPKPDA
                     LLKRAGLI"
     gene            complement(213..1328)
                     /locus_tag="PK218_10400"
     CDS             complement(213..1328)
                     /locus_tag="PK218_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW98961.1"
                     /translation="MISRDLTSVLATKLHKGKVIVLIGPRQVGKTTMIKTVLADNDYL
                     FLDGDDHSVAEIISNANTEALRSIIGTYKYVFIDEVQRIPNIGLKLKIIVDQIKNVQV
                     IVSGSSAFDINNATQEPLTGRKFEYNLYPISWNEFEKNVGYIKAQQQLELRLLYGMYP
                     DVINNFGEEHEILKNLVSSYLYKDILALAGIRKSEYLEKVVQALAFQVGNEVSYNEIA
                     QLVGIDKNTVSNYIDLLEKSFVVFRINSFSKNLRNEIKANKKIYFYDNGVRNMIIGNF
                     NNLALRQDKGALWENFMMAERMKFLSYSQSNAKSYFWRTTTQQEIDYVEINADEVAAF
                     EFKWATNKKVKLPKSFATAYEPNFMVVNKDNFREFLK"
     gene            1520..2023
                     /locus_tag="PK218_10405"
     CDS             1520..2023
                     /locus_tag="PK218_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HPW98962.1"
                     /translation="MEFKDAKNKFVQTWGALGSQWGINKTMAQIHALLMVSIEPVSME
                     DIMEELQISRGNASMNLRALMDWGIVYKEYKAGERREFFTAEKDLDELAIKIAKERSK
                     REIKPALKVLKEVSTIDSNSTEEEQHFKNQTTQLYDFVLKTDNILEKITEYKDNWLAQ
                     LLVRFMK"
     gene            2077..2790
                     /locus_tag="PK218_10410"
     CDS             2077..2790
                     /locus_tag="PK218_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HPW98963.1"
                     /translation="MSFLKAQWENLALINYEIDHEILKNYIPKGTEIELWNGKCYVSL
                     VGFMFKNVKLLGLKIPFHINFEEVNLRFYVKSFENNEWKRGVVFIKEIVPKQALTFVA
                     NTIYKEHYQTLSMKHSIQESTDSTEFTYEWKTANKWNSILIETEKNETEIEINSEAEF
                     ITEHYFGYTKINENKTFEYEVKHPKWQQLKVINTKIDVDFKATYGKEFEFLENQKPVS
                     VLLAIGSEISVENKKTKKL"
     gene            2787..3227
                     /locus_tag="PK218_10415"
     CDS             2787..3227
                     /locus_tag="PK218_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98964.1"
                     /translation="MNLNIPNWLIITSGIGQIFTALIYPYIRHQVFDWYNDVKQLKPL
                     NQEIAKTYGRYIQGLNFSFGLIAILLPNDLKNQSQLAIALTGLIAAYWVGKVATQIAY
                     YPMYDIPKRSLFKVGSYFMNSLFVLFAIVNTLLFIYNLIGYYKF"
     gene            3597..4049
                     /locus_tag="PK218_10420"
     CDS             3597..4049
                     /locus_tag="PK218_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPW98965.1"
                     /translation="MTKIHLITKINAPINQVFDLARNIDVHQQSTASSYEKSIDGITS
                     GLINLNETVTWRGKHFGIYLKHKSLISEMEFPTYFVDEMLEGHFKSFRHEHNFVEKNE
                     KTVMIDTIEYQTPFGMLGRIFDQLVLKNHLTQFITERNKFIKKLAESH"
     gene            complement(4032..4847)
                     /locus_tag="PK218_10425"
     CDS             complement(4032..4847)
                     /locus_tag="PK218_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98966.1"
                     /translation="MNFFKKILDFYIKSSLHVAFSVFALVHMTGFLFRIINIESCAYF
                     AFFGTIVGYNFVKYDALARTQKVNMSLSLKVIAFLSFISLVATMFFFLKLETITKILG
                     LVFLILTLLYTLPFFPNKRNARNWAGIKIYIVTLCWVGVTLVLPIINAQVPIDNYVFL
                     VAMQRFVFVFVLVLIFEIIDLRQDDPHLQTVPQKIGVKNTKIIGVSLMIVFVFLEYFD
                     SNFKQEILLLKLGIAITTSLFLLFANEKKSKYYSSFWVESIPIFWWVLLVTFS"
     gene            complement(4884..>5221)
                     /locus_tag="PK218_10430"
     CDS             complement(4884..>5221)
                     /locus_tag="PK218_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98967.1"
                     /translation="VLQTATNYEVTATNSGGSVSFTLSIAVEEELGIDDNIKLDFKVY
                     PNPFLDEINVIHAFNEVKFEIYSLEGKEIQKGMLQNSKIVVNQLPSSVYLLKLTSEKA
                     YQIIKIVKK"
BASE COUNT         1932 a          776 c          715 g         1798 t
ORIGIN      
        1 tcttccaaga aaaaggaatt tttgacaaag aagttgccac aaaattcaaa gacaatatct
       61 tatctaaagg cggaaccgaa ttgcctatgg aattgtataa aaaattccga ggaaaagaac
      121 caaaacccga tgcattgttg aaacgtgctg ggttgatata atttttaagc ctaacaggat
      181 ttaaaaacct gttagtctta aattattgct atttacttca aaaactctct aaaattatct
      241 ttattaacaa ccataaaatt gggttcgtaa gcggttgcga atgattttgg taattttact
      301 tttttgttag ttgcccattt aaattcaaaa gcagcaactt catcggcatt aatttccaca
      361 taatcaattt cttgttgagt tgtagttcgc caaaaatagg attttgcatt actttgtgaa
      421 taagatagaa acttcatacg ttctgccatc ataaagtttt cccataaagc acctttatct
      481 tgtcgaagtg ctaaattatt gaagttaccg ataatcatat ttcgaacacc attatcataa
      541 aaataaattt tcttgttggc ttttatctca tttcgaaggt ttttactaaa actattgatc
      601 ctaaaaacca cgaaactttt ttctaacaaa tcaatataat tactaaccgt atttttatca
      661 atacctacca attgagctat ttcattataa ctgacttcgt ttccaacctg aaaagctaat
      721 gcctgaacta ctttttccaa atattctgat tttctgattc ctgccaatgc cagaatatct
      781 ttgtacaaat aactcgaaac caagtttttc aagatttcat gttcttctcc aaaattattg
      841 atgacatcag gatacattcc atataataat cgcaattcta gttgttgttg tgctttgatg
      901 tagcctacat ttttttcaaa ttcattccaa gaaataggat ataaattgta ctcaaatttt
      961 cttccagtta aaggttcttg tgtagcatta tttatatcaa aagctgatga accactaaca
     1021 ataacttgaa cattttttat ttgatcaaca atgattttca actttaaacc tatatttgga
     1081 atacgttgta cttcatcaat aaacacatat ttatacgttc caatgatgct tcgcaaagct
     1141 tccgtattag cattactgat tatttcagcc acagaatgat catcaccatc taaaaaaaga
     1201 taatcgttat cagccaaaac tgttttaatc atcgttgttt ttccaacttg tcgtggtcca
     1261 atcaacacaa ttactttgcc tttatgtagt ttcgttgcta aaaccgaagt taaatctcta
     1321 gaaatcatct tgtaaaattt taacaaaaat ataaaatatt atttctattc accaatttta
     1381 taagtttttt agtgaacgta ttcaccaatt ttatatattt tttagtgaac gtattcacca
     1441 atattacatt tatcaatata aattgaaata actttcattt ttttttgaaa gttcaaaaac
     1501 tttgtagtac atttgcttca tggaattcaa agacgcaaaa aataaattcg ttcaaacttg
     1561 gggtgcattg ggtagtcaat ggggaattaa taaaactatg gctcaaattc acgctctatt
     1621 aatggtttca atagaacctg tttctatgga agacattatg gaagaattgc aaatttctcg
     1681 tggaaatgct tctatgaatt tacgtgcttt gatggactgg ggaattgttt acaaagaata
     1741 taaggctggt gaacgaagag agttttttac tgctgaaaaa gatttggatg aactagccat
     1801 aaaaattgca aaagaaagaa gcaaaagaga aataaagcct gctttgaaag ttttaaaaga
     1861 agtttctact atagattcta actcaaccga agaggaacaa cattttaaaa atcaaacaac
     1921 acaattgtat gattttgttt taaaaacaga taatatactc gaaaaaatta ccgaatacaa
     1981 ggacaattgg ctagctcaat tgcttgttcg atttatgaag taaaaaaatt taattaaaac
     2041 tttcaatttt ttttgaaagt ttagaaataa aacattatga gtttcttaaa agcacaatgg
     2101 gaaaatttag ctttaatcaa ttatgaaatt gaccatgaaa tcttaaaaaa ctatattcca
     2161 aaaggaaccg aaatagagtt atggaatgga aaatgttatg ttagtttagt aggatttatg
     2221 tttaaaaacg taaaattatt aggattaaaa attccgtttc atattaattt tgaagaagta
     2281 aatcttcggt tttatgtaaa aagttttgaa aataatgaat ggaaacgtgg tgttgttttc
     2341 attaaagaaa ttgttccaaa acaagccttg acttttgtcg caaatacaat ttataaagaa
     2401 cattatcaaa cactttcaat gaaacattct attcaagaaa gcacagattc aactgaattt
     2461 acttatgaat ggaaaactgc aaacaaatgg aactctattt taattgaaac agaaaaaaac
     2521 gaaacagaaa ttgaaataaa ctctgaagct gaatttatta ctgaacatta ttttggttac
     2581 acaaaaataa acgaaaataa aacatttgaa tatgaagtaa aacatcccaa atggcaacaa
     2641 ttaaaagtca ttaatactaa aatagatgta gactttaaag caacttatgg gaaagaattt
     2701 gaatttttag aaaatcaaaa accagtttct gttttattag caattggttc agaaattagt
     2761 gtagaaaata aaaaaactaa aaaattatga acttaaacat tccaaactgg ttaattatca
     2821 caagtggtat tggtcaaatt tttacagctt taatatatcc ttacattcgt catcaagttt
     2881 ttgattggta taacgatgta aaacaattaa aacctttaaa tcaagaaatc gcaaaaactt
     2941 atggaagata tattcaagga ttaaacttct cttttggatt aatagcaatt cttttaccaa
     3001 atgatttgaa aaatcaatct caattagcca ttgcattaac tggattaatt gcagcttatt
     3061 gggttggaaa agttgctaca caaattgcct attatccaat gtatgatatt ccgaaaagat
     3121 cactttttaa ggtaggaagc tattttatga atagtttgtt tgtccttttt gcaattgtta
     3181 atactttact tttcatctat aacttaattg gctattataa attttaattt tatgaattgg
     3241 agttatttat ttaagcattg gactttcaga atactacttt caccatttgt gtatgatcta
     3301 attcaatatt ttgttttaaa atctaatggt gtttaaggat ttggtgtaat ttatccaata
     3361 gtaattattt ttagcttaat tttttcttct cctacccatg ttgtttatgc tttcttattt
     3421 tattttctaa agaagaagaa aatcaattta gttttatcaa aaataatact cattacattc
     3481 tatgctttag gagtgattgc ttctttttta ataatatatg actcagatga ataagagttg
     3541 actattccat atgtgataac ttctataatt tcaggtttgt actttaaatt aaaaaaatga
     3601 ccaaaatcca tctcatcaca aaaataaatg ctcccataaa tcaagtcttc gatttagccc
     3661 gaaacattga tgttcatcaa caatctacgg cgagttcata tgaaaagtca attgatggca
     3721 ttacttcggg attaattaat ctaaatgaaa ccgttacatg gcgtgggaaa cattttggaa
     3781 tttatttaaa acataaaagt ttaatttctg aaatggaatt tccaacttat tttgttgatg
     3841 aaatgttaga aggtcatttt aaaagtttta gacatgaaca taacttcgtt gaaaaaaatg
     3901 aaaaaacagt gatgattgat accatagaat atcaaactcc ttttggaatg ttaggcagaa
     3961 tttttgacca attagtatta aaaaatcatt tgactcaatt tataacagaa cgaaacaaat
     4021 tcattaaaaa attagctgaa agtcactaac aaaacccacc aaaaaatcgg aatactttct
     4081 acccaaaagc tagaatagta tttcgatttt ttttcgttag caaataacag aaaaagcgac
     4141 gttgttatag caattccaag ttttaataaa agaatttctt gcttgaaatt agaatcaaaa
     4201 tattcaagaa agacaaatac aatcatcaat gaaaccccaa ttattttggt gttttttact
     4261 cctatttttt ggggtactgt ttgcaaatgc ggatcgtcct gacgtaaatc gatgatttcg
     4321 aaaattaaaa ctaaaacaaa gacaaaaacg aatcgctgca ttgcaaccag aaaaacataa
     4381 ttatcaattg gaacttgagc attgataata ggcaaaacta gtgtaactcc aacccaacag
     4441 agcgtaacga tatagatttt tattcctgcc caattccgag catttctttt attaggaaag
     4501 aaaggcaacg tatataacag tgtcaaaatc aggaaaacca atccaagtat tttggttatc
     4561 gtttctagtt tcaagaaaaa aaacattgtt gctactaaag aaataaaact caagaatgct
     4621 attactttca agctcaaact catgtttact ttttgagttc gggctaaggc atcatatttc
     4681 acaaaattgt aacctacaat agttccaaag aaagcaaaat aagcacagct ttcaatgttg
     4741 attattcgaa acaaaaaacc agtcatgtgc actaatgcaa aaactgaaaa agcaacatga
     4801 agactgcttt taatataaaa atcaagtatt tttttaaaaa aattcataaa aaaaaggagt
     4861 ttgactagca aactcccttt aaattatttt ttaacaattt taataatttg atatgctttt
     4921 tcggaagtta gctttaacaa atatacgctt gatggtaatt gattcactac tatttttgaa
     4981 ttttgcaaca ttcctttttg aatttccttt ccttccaaac tataaatttc aaatttaact
     5041 tcgttaaaag cgtgaataac attaatttca tccaaaaatg gatttggata aactttaaaa
     5101 tcaagcttga tattatcatc aattcctaat tcttcttcaa ctgcaattga taacgtaaag
     5161 gaaacacttc cgcctgaatt ggttgccgtt acttcataat tggttgccgt ttgtagaact
     5221 g
//
LOCUS       DAOOBH010000130         5143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_18464_length_5143_cov_7.106918, whole genome shotgun sequence.
ACCESSION   DAOOBH010000130 DAOOBH010000000
VERSION     DAOOBH010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18464_length_5143_cov_7.106918"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..349
                     /locus_tag="PK218_10435"
     CDS             152..349
                     /locus_tag="PK218_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98968.1"
                     /translation="MTKKFLLHKDKEEEGKASPQKQECGCPSLQPSLRSHTHAFLAAH
                     GRHEHKTVKATTINGAKTINL"
     gene            354..1094
                     /locus_tag="PK218_10440"
     CDS             354..1094
                     /locus_tag="PK218_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98969.1"
                     /translation="MYNEQLEKLIEMALMDGELTEKEKQVLFKKAEAMGVDLDEFEMV
                     LEAKLYEKTQAMNQETEAKAAPKSDKFGDVKKCPACGAMVQSFQSKCPDCGHDYSNIE
                     SNASIQKLFKMLDDVESGRKDDEGMDPLKAFGGAMAKGFGMGGVGDKINNRKKEIIKN
                     FPIPTTKDDILEFLSLAIPNARKQGNFFTSGAFNSPANERNKLHNDFVPIWKAKCEQI
                     VLKARFSMKEDKSILEEINNYARELGIK"
     gene            1104..1646
                     /locus_tag="PK218_10445"
     CDS             1104..1646
                     /locus_tag="PK218_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98970.1"
                     /translation="MNEKIIKLVEYALADGEISDKELQVLYKKCDENGIKHDELENYI
                     STHLKGKSEVFTPNDSKISDELETAIQRLDAITVSIKTAPKASRFSIQFRIVAILMTF
                     GIYHIISMLSKKSSLGFHGEKVYYDNINEIKTIKEKYGNHELIISQFKVLNKVTLKYK
                     IINYMILSVIVIAFLSLFFS"
     gene            1665..3020
                     /locus_tag="PK218_10450"
     CDS             1665..3020
                     /locus_tag="PK218_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPW98971.1"
                     /translation="MGLFGNKSEGGLMDVIRCDEQEYLVWKWRPSGEANSTKKENSIR
                     YGSSLRVKDGELAVFVYQQKDGTMQDFIIGPYDQTIKTANFPVLTSIVGAAFGGSSPF
                     QAEIYFINLSGNVQIKFGIPYFDVFDPRFLDFAVPMAVRGTITFNITDYKGFIKLNRL
                     INFELDDFKKQIKDAVTKYIKGVVTNIPADNGIPVLQMERKILEINDLVAKYLGSRLE
                     TDFGVNMKGLDIAALEVDKEAEGYAELRKVTAEQTTKTVGAQTDVNIKNLADTQEINA
                     KNMEETLRIQREEAQRAQKLQTETQNISAHQLNQQTEVAKTAAESMGKMGSGGSGGSG
                     GGGGMMDPGSMMASMAMGGAVGSGMAGAVGGMMQGMNEQKQTPPPPPQVQYNISVNGQ
                     QSGPFGWQQLQQMVQSGQITKDTYVWKQGMANWELAGNVQELSSLFGAVPPPPPPPPA
                     V"
     gene            3061..4077
                     /locus_tag="PK218_10455"
     CDS             3061..4077
                     /locus_tag="PK218_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98972.1"
                     /translation="MENLNFKSARFWPLFSNIFFPAELTLDNEFAYYRKKRLFFKPSQ
                     VSVNIKEIAFFDTSGIFNKTLEFGYTDQILMKGISSKNCNIIKKHIIANGAKIGKEGF
                     VVKSLPFTGLSDLLKPLKWFLRERLILTDDAVLFIKRKLFSNKTTYLPFGKISFGYID
                     GWLSKKIYIIGEQNIMPSYSFPSKPISTLKAKLKEIGLELKKGKSFRPFWFSSGRNLV
                     NPPRIFCTEKEIAYIDVLPFKDTVIKVLRYNEIETFKKRKWYSLLGSIVISGNVENIR
                     GQSTIEYSREIKKEMESEKPVIKDDEFKNNIVILLKNVWFYRWRVFFISTGIRAYIKN
                     HKNQ"
     gene            4079..4432
                     /locus_tag="PK218_10460"
     CDS             4079..4432
                     /locus_tag="PK218_10460"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HPW98973.1"
                     /translation="MDDNSFFSIDRLVEFGMGMAVAQQMVKTMNESMTNMHIPGAMNS
                     MEKPKEKFFYAMIEGNQAGPFSEQELARLIAEKKVSKETYIWMPSLPNWKMAEQIPEV
                     LKLVALTPPPFQQNK"
     gene            4436..4780
                     /locus_tag="PK218_10465"
     CDS             4436..4780
                     /locus_tag="PK218_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98974.1"
                     /translation="MKINLVQTIIAIAVSALIAYGLYNFHDGENQILLSVGSFVFLAS
                     TLVLTIGTSFQLPRTTTNVRVVSGIFFAVALISNLIFTFVEFSVPSYVITNGILLLVF
                     ILIAYSINRAKQ"
BASE COUNT         1856 a          820 c          972 g         1495 t
ORIGIN      
        1 cacgcggtat agttaattgc cggtgcagtg ggtattcgag cggcacagct cgtattaaaa
       61 gtagttgtaa cttgatagga aagtgcttcg aaatcggcaa ctaaccatac cgccaaacgt
      121 tacaagcaag ccgaaaaaag acacagacac aatgacaaag aaatttttgc tgcataaaga
      181 caaagaagaa gaaggaaaag ccagcccaca aaagcaagag tgtggctgcc cttccctgca
      241 accgtccctt cgcagccaca cacatgcttt tttagcagcc cacgggcgac atgaacataa
      301 aacggtaaag gcgaccacca ttaacggagc caaaacaatt aatttataag aatatgtata
      361 acgaacaatt agaaaaattg attgagatgg ctcttatgga tggagagtta actgaaaaag
      421 aaaaacaagt gctttttaaa aaagctgaag caatgggtgt tgaccttgat gagtttgaaa
      481 tggttctgga agcaaagctc tatgagaaaa ctcaagcaat gaatcaagaa acagaagcga
      541 aagcagcacc aaaatctgat aaatttggtg atgtaaaaaa atgtccggca tgtggtgcaa
      601 tggttcagtc atttcaatct aaatgtcctg attgtggtca tgattactca aatatcgaat
      661 caaatgcttc aattcaaaaa ttatttaaaa tgctagatga tgttgaatct ggtagaaaag
      721 atgatgaagg tatggatcca ctaaaagctt ttggcggtgc tatggctaaa ggttttggca
      781 tgggtggtgt tggtgataaa ataaacaatc gtaaaaaaga aataattaaa aactttccta
      841 ttccaactac aaaagatgat atccttgaat ttctatcact cgcaatcccg aatgctagaa
      901 aacaaggaaa tttctttact tctggtgctt ttaactctcc tgccaatgaa agaaataaat
      961 tacataatga tttcgtgcct atttggaaag ctaagtgcga gcaaattgtc ctgaaagcaa
     1021 gattctctat gaaagaagat aaaagtatac ttgaagaaat taataattat gccagagaat
     1081 taggcatcaa ataatgtgaa attatgaatg agaaaataat aaaattagtt gaatatgctc
     1141 ttgctgatgg ggaaatatct gataaagaat tacaagttct ttataaaaag tgtgatgaaa
     1201 atggaataaa acatgatgag ttagaaaatt atatttcaac ccatttaaaa ggcaagtctg
     1261 aagtgtttac gcctaatgat tctaaaattt cggacgaact tgaaactgct attcaaagat
     1321 tagatgcaat tactgtttca ataaagacag caccaaaagc atctcgtttt tctattcaat
     1381 ttagaattgt cgcaatttta atgacttttg gtatttatca tataatatca atgctttcaa
     1441 aaaaatcatc tttaggcttt catggtgaaa aagtatacta tgataacata aatgagatta
     1501 aaacaattaa agaaaagtac ggaaatcacg aacttattat ttctcagttt aaggtactta
     1561 acaaagttac tcttaaatac aaaattatta actatatgat tttatctgtg attgttattg
     1621 cattcttatc tctattcttt tcgtaaaact ttaaaaaata atatatggga ctattcggaa
     1681 acaaatcaga aggcggatta atggatgtaa tccgctgtga cgaacaagaa tatcttgttt
     1741 ggaagtggcg accttcggga gaagccaata gcacaaaaaa agaaaactca atccgttacg
     1801 gaagtagttt acgggttaag gatggtgaat tagccgtttt tgtttatcaa caaaaagacg
     1861 gcacaatgca agattttatt ataggacctt atgaccaaac aataaaaact gccaactttc
     1921 ctgttttaac aagcattgta ggtgccgctt ttggtggttc atcaccattt caggcagaaa
     1981 tctattttat aaatttatct gggaacgtac aaatcaagtt tggcatccca tactttgatg
     2041 tgtttgaccc aaggttttta gactttgcag ttccaatggc tgtacgcggt actataactt
     2101 ttaatatcac agactacaaa ggatttataa agctaaaccg tttaattaac tttgaattag
     2161 acgactttaa aaagcaaatt aaagatgctg taactaaata tattaaaggt gttgtaacta
     2221 acataccagc agacaatgga atacctgttt tacaaatgga aaggaaaata cttgaaatta
     2281 atgatttagt tgcaaagtat ttgggtagca gacttgaaac ggattttggt gtaaatatga
     2341 aaggtttgga tattgcagca cttgaagttg acaaagaagc tgaaggttat gctgaattaa
     2401 gaaaagttac agcagaacaa acaacaaaaa ctgttggtgc tcaaactgac gtaaatatta
     2461 aaaatctcgc agatacgcag gaaattaatg ccaagaacat ggaagaaact cttcgtatcc
     2521 aaagagaaga agcacaaaga gctcaaaaac tacaaacaga aactcagaat atttctgctc
     2581 atcaattaaa tcaacaaaca gaagttgcta aaacagcagc agaaagcatg ggaaaaatgg
     2641 gttctggtgg tagtggtggc tctggcggcg gaggcggtat gatggatccc ggctcaatga
     2701 tggcaagtat ggcaatgggc ggtgcagttg gctctggcat ggcaggtgct gtcggtggaa
     2761 tgatgcaagg aatgaacgag caaaaacaaa caccaccacc tcctccacaa gttcaatata
     2821 atatttcagt aaatggtcaa caaagtggcc cgtttggttg gcaacaattg caacagatgg
     2881 tacaaagtgg acaaattacc aaagacactt atgtatggaa acagggtatg gcaaattggg
     2941 aattagcagg aaatgttcag gaattatcat cattatttgg ggcagttcct ccacctcctc
     3001 cgccaccacc tgctgtctaa attatattat tactaagtag caaacttaat tattaaacat
     3061 atggaaaact taaattttaa atcagcacgt ttttggcctc tttttagcaa tatctttttc
     3121 cctgcagaac ttacgctgga taatgaattt gcatattaca gaaaaaaaag gctctttttc
     3181 aaacccagcc aagtttcagt aaacattaaa gagattgctt ttttcgatac cagcggtatt
     3241 tttaataaaa ccttagaatt tggctatacc gaccaaatat taatgaaagg gattagtagc
     3301 aagaattgta atattattaa aaagcatatt attgcaaacg gggctaaaat aggaaaagaa
     3361 ggatttgttg ttaaatcatt accttttaca ggtctttcag atttactcaa accattgaaa
     3421 tggtttttaa gagaaaggtt aattttaacc gatgacgctg ttttgtttat aaaaaggaaa
     3481 ctgttctcaa ataaaacaac ctatttgcct tttgggaaaa tttcatttgg ttacattgat
     3541 ggttggctat caaaaaagat ttatataatt ggtgaacaaa atataatgcc ttcatattct
     3601 ttcccaagta aacctattag tacattaaag gcaaaattaa aggaaatagg tttagaactg
     3661 aaaaagggta agtcttttag accattctgg ttttcttctg ggagaaatct tgtaaatcca
     3721 ccccgaatct tctgcacaga aaaagaaatt gcatacatag atgttttacc ttttaaagat
     3781 accgtgataa aggttttgcg ttataacgaa attgaaacct ttaaaaaacg taaatggtat
     3841 tctttgcttg gcagtattgt tatctctggt aatgttgaaa atattcgtgg acaatcaact
     3901 attgaatatt ctcgtgaaat aaagaaggaa atggaaagcg agaaaccagt gattaaagat
     3961 gatgaattca aaaacaacat cgtgatttta ctaaaaaatg tttggtttta tagatggcgt
     4021 gtgtttttca tttcaactgg cataagagct tacattaaaa accataaaaa tcaataatat
     4081 ggatgataat agttttttct caatagacag attagttgaa ttcggtatgg gtatggctgt
     4141 tgctcagcaa atggttaaaa ccatgaatga atcaatgact aatatgcaca ttccaggtgc
     4201 aatgaactca atggagaaac caaaagaaaa gtttttctat gctatgattg aaggcaatca
     4261 agcaggccca ttttctgaac aagaattggc tcgcttgata gcagaaaaaa aagtttctaa
     4321 agaaacttat atctggatgc cttctttgcc aaattggaaa atggcagaac aaataccaga
     4381 agttttaaaa ttagttgcgt taactcctcc accatttcaa caaaataaat agcttatgaa
     4441 aataaatctt gtacaaacaa taatagccat agcggtaagt gctcttatcg cttatggctt
     4501 gtataatttt cacgatggtg aaaaccaaat acttttaagt gttggcagtt ttgtgtttct
     4561 tgcgtcaact ttggtgctta caatcggaac aagttttcaa ttgccacgaa caacaacaaa
     4621 tgtcagagtt gtatcgggga ttttctttgc tgtggctttg attagcaatt tgattttcac
     4681 gtttgttgaa ttttcagtcc caagttatgt gattacaaat ggtattttac tattagtttt
     4741 tattttgatt gcatactcaa taaacagagc aaaacaatag ccaacgcttc gtagtcaagc
     4801 acatttgcag gtcgcacaag ccgacgcact tccaaaacct gcaaaagagc ttgcctaccc
     4861 aaacgcactg atagaaaaat atgcagcaaa aatgaaagac agaataacga atgacaagaa
     4921 cggccagctt gtaacatgcg gtatatttta ttgccgagat agtgctgata tcaacggctg
     4981 tggctcgctt caaactttat cgtggtttga cagtgacgtg ctccgtaatc ggcaacaaaa
     5041 acataccgct atccgttaga ggtaataagg gctttgttaa aatatgaaaa ataaaagctt
     5101 agaatcaata acttcaatac ataatttaag tactgcctgt tcg
//
LOCUS       DAOOBH010000131         4901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_19594_length_4901_cov_5.375980, whole genome shotgun sequence.
ACCESSION   DAOOBH010000131 DAOOBH010000000
VERSION     DAOOBH010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19594_length_4901_cov_5.375980"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1337)
                     /gene="paaK"
                     /locus_tag="PK218_10470"
     CDS             complement(264..1337)
                     /gene="paaK"
                     /locus_tag="PK218_10470"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HPW98975.1"
                     /translation="MAKFHNIQVQDIHKETRDCSVITFKIPEELAEEFQYKQGQHLTL
                     KSIIDGQDVRRSYSLCSSPVENKWQVAVKKINGGLFSSFVNEKLKAGDSIEIMAPSGK
                     FNVEIDSEKSKNYILFAAGSGITPILSILKTHLAKEPNSTFKLFYLNRSVKSIIFKEE
                     IEQLKNSYFGRFEIFYFLTKEHRNIEWLNGRFTKEKLEILTSKIIDIPSVDECFICGP
                     EEMIFLIRDELVAAGLEKDKIHYELFTSGISDEDKERIKRIVEHKAEGTDVTIIDGGK
                     EFHFVMDEAYDNLLDGALAAGADLPFACKGGVCSTCKCMVIEGEVEMKVNYALDAHEV
                     AKGLVLSCQAVPTTDKVVVDFGY"
     assembly_gap    1422..1521
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1594..2166)
                     /locus_tag="PK218_10475"
     CDS             complement(1594..2166)
                     /locus_tag="PK218_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPW98976.1"
                     /translation="MKIIDRKTEIINCAAKLFKEKGYSAVTMRDIAQALDIKAASLYN
                     HIKSKQEILVLIIIEIAEEFTLTMNSIVNSEISTIQKLEKVIQLHIEITIRNPEALAC
                     LNNDWMHLTDNELIYFIKMREDYEENFRNIIKSGIAANEIKNLNQEVIIFSMLSTLRT
                     LYLWYGKKKGFTETSLKNNLKEVLLNGIVN"
     gene            2263..2571
                     /locus_tag="PK218_10480"
     CDS             2263..2571
                     /locus_tag="PK218_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPW98977.1"
                     /translation="MGILSMIGLSGKSESVQEFMNKGAVIIDVRTVGEFREGHIKNSK
                     NIPLDTIFSKVEEIKKLNKPVIVCCRSGMRSGQANSILKNSGIESMNGGGWQSLEGKL
                     "
     gene            complement(2610..3140)
                     /locus_tag="PK218_10485"
     CDS             complement(2610..3140)
                     /locus_tag="PK218_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98978.1"
                     /translation="MKTINKFGLILLTSISVLLSSCSKSDSGGSSIPETGSYIQANVA
                     GSNFQSSGDFFSGTFSSSSMNISGTSTDGKSLNIQLYPLSGTTLTTGTYNINSTSQNG
                     GGLTFIDVNTTTFSVVTFSSVNCDNSIGTLEITYIDSAKIEGTFSCAAKEVKADESCN
                     GDTKNITNGSFRLLLN"
     gene            complement(3215..3865)
                     /locus_tag="PK218_10490"
     CDS             complement(3215..3865)
                     /locus_tag="PK218_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW98979.1"
                     /translation="MMIKLAIVDDNSFLIQATRQKLALFSDFIIKCTAINGIEIIREL
                     EKNSNYDLILMDIEMPKLDGIGATEIIKQKFPHIKIIMLTVFDNDANIFNAIKAGADG
                     YLLKEVSPDELKKGILETLNGGATMTPSIAMKTLNLFRNPTLIEPSTIDENIKLTTRE
                     IEVLEQLSKGLKYDAIANNLILSSGTIRKHVENIYNKLKVHNKLEAIQKAKNNNLI"
     gene            complement(3859..>4901)
                     /locus_tag="PK218_10495"
     CDS             complement(3859..>4901)
                     /locus_tag="PK218_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPW98980.1"
                     /translation="GVKNNSRSQVVKKEYSNEPAYFEDLIQTYNYLIQTSSQLNQKDS
                     VSFYSNAIMRLQDKIIEQTVRKQTNEFETKYQTVKKEKLIAQQKEEVKYKNILLLGLT
                     IIVFFIALFGFLIFRQQKLKNKQQKQEFELKSAITQIEKQNELQQQRLSISKDLHDNI
                     GAQLTFIISSVETAKYVADIKNTTLESKLSNISNFTKDTIVELRDTIWAMNSETINFE
                     DLRLRILNFIEKATTATTDISFNFMVDNALKNTHLSSVQGMNVYRCIQEAINNAIKYS
                     EANNILVEVILDKNEIKIIIKDNGKGFDVETISNGNGLQNMQKRMEEIGGTFQIVSEP
                     TKGTQIKLNFKS"
BASE COUNT         1492 a          731 c          712 g         1866 t
ORIGIN      
        1 tcttttggtt cgattttttc gtcgcgtgca atttttgcat cgaaaatttc ttctaggttt
       61 tttacttctt tttctgacat aacttttttt tattagttat tattttagcc acagattctt
      121 tactataaat acgtggatta ttttattttt aattcaatac atttcccaat tagccctgat
      181 tgcagtgaaa atcctttttt gtaaaaaaag attgtagcgg aaagcaggaa aatggacaac
      241 taaaatgccc aagctaatcg ctactaataa ccgaaatcta ctactacttt atcggttgtt
      301 ggcacagctt gacagcttaa gaccaatcct tttgccactt cgtgagcatc gagagcatag
      361 tttactttca tttctacttc gccttcaatc accatgcact tgcatgtgct acaaacgcca
      421 cctttgcaag caaaaggcaa atcagcacca gcagctaaag caccatcgag taaattgtcg
      481 tacgcttcat ccatgacgaa atggaattct tttccgccat cgattatggt aacatcggtt
      541 ccttcggctt tgtgctcaac aattcgtttt atgcgttctt tgtcttcatc tgaaattcca
      601 gatgtaaaca attcgtaatg tattttatct ttttctaaac cagcagcaac taattcatca
      661 cgaattagaa atatcatctc ttctggtccg caaatgaaac attcatctac tgaaggaata
      721 tctataattt tactggttag aatttctagc ttttctttag taaatctgcc gttcaaccat
      781 tcaatatttc tatgctcttt tgttaagaaa taaaatattt caaaacgacc gaagtagctg
      841 tttttgagtt gttctatttc ttctttaaag ataattgatt ttacacttct attcaaataa
      901 aacaacttaa aagtactgtt tggttctttt gctaagtgtg tttttaggat agaaagtatt
      961 ggtgtgattc cgcttcctgc tgcaaaaaga atataatttt tagatttttc agaatcaatt
     1021 tcaacattga atttgccact tggagccata atttcgattg aatctccagc ttttaatttt
     1081 tcgtttacaa aacttgaaaa caaaccacca tttatttttt taacggcaac ttgccattta
     1141 ttttcaacag gacttgaaca taatgaatac gaacgacgaa catcttgacc gtcaattatt
     1201 gattttaatg ttaaatgttg cccttgtttg tattgaaatt cctcagctaa ttcttctgga
     1261 attttaaagg taataacaga acaatctctt gtctctttat gaatatcttg gacttgtatg
     1321 ttgtgaaatt ttgccattca aatttgtaaa ataatttaac taacacttgt tagtttacaa
     1381 tgcaaattta ctgaaaatat taattagtta cgaaaacgtt gnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nttgttagtt tacaatgcaa atttactgaa aatattaatt
     1561 agttacgaaa acgttgtaaa tttttaactt ttgttaattt acaattccat taagtaaaac
     1621 ttcttttaaa ttatttttca aagaggtttc cgtaaagcct tttttctttc cataccataa
     1681 ataaagtgtt ctaagtgttg aaagcattga aaatattata acttcttgat ttaggttttt
     1741 aatttcgttt gcagcgattc cggatttgat aatatttctg aaattttctt cataatcctc
     1801 acgcattttt ataaaataaa tcagttcatt atctgttaaa tgcatccagt cgttgtttaa
     1861 acaagccaat gcttctgggt ttcgtattgt aatttcaata tgtaactgga ttactttttc
     1921 taatttttga atggtggaaa tttcagaatt aactatggaa ttcattgtta aagtaaattc
     1981 ttcggcaatt tcaataatga ttaataccag aatctcttgt tttgacttga tgtgattgta
     2041 caaacttgcg gctttaatat ctaaagcttg cgcaatatct ctcatcgtta cggcactata
     2101 tcctttttct ttaaaaagtt ttgcagcgca atttataatt tctgtttttc tatcgataat
     2161 cttcatactt gtcaaatata tagattttat agttacgtaa cttttgttac ttcataactt
     2221 agtaaggcat tgtatttttg tcaaaaatta aaaattaaaa aaatgggaat attaagcatg
     2281 ataggattaa gcggaaaatc ggaaagcgtt caagagttta tgaacaaagg agctgtaatt
     2341 attgatgtta gaacagtggg agaatttaga gaaggtcata ttaagaattc taaaaacatt
     2401 cctttagaca ctattttttc taaagttgaa gaaataaaaa aattaaataa acctgtaatt
     2461 gtgtgttgta gaagcggtat gagaagtggg caagcgaatt ctattttaaa aaatagtggg
     2521 attgagtcta tgaatggtgg cggttggcag agtttggaag gaaaattata aaaaatggct
     2581 tccgatattt ggaagccatt tacagtgttt tagtttaaca acaatctaaa agaaccattg
     2641 gtaatgtttt ttgtgtcacc attgcaactt tcatcagctt taacctcttt tgcagcacag
     2701 gaaaaagtgc cttcaatttt agctgagtcg atataggtaa tttctaatgt tcctatagag
     2761 ttatcacaat ttactgaact gaatgttact actgaaaaag tagttgtatt aacatcaata
     2821 aaagtaagtc cgccaccgtt ttgacttgtt gaatttatat tatatgttcc tgtagttaat
     2881 gttgttcctg ataatgggta cagttggata tttaaagatt ttccatctgt tgaagttccc
     2941 gatatattca tagaagaact actaaaagta ccactaaaaa aatctccaga agactggaaa
     3001 ttactacctg cgacatttgc ctgtatgtaa ctgcctgttt caggaattga acttccacca
     3061 ctgtcacttt tactacatga acttaacaag actgaaatgc ttgttaaaag aattaatcca
     3121 aatttgttga ttgttttcat attgatattt gtttaagatt atgaggcaaa gatttaattt
     3181 ttcaaacctt aaaaccatac gaaaaaattc gtatttatat caaattatta ttttttgctt
     3241 tttgaatagc ttctaattta ttatgcactt ttagcttgtt ataaatattt tcaacatgtt
     3301 ttctaatcgt tccagatgac aaaattaaat tgttagcaat agcgtcatat tttagacctt
     3361 tgctcaactg ttctaaaact tcaatttctc tagtggtaag ttttatattt tcatctattg
     3421 ttgaaggttc gattaatgtt gggtttctga ataaatttag cgttttcatg gcaatgcttg
     3481 gtgtcatggt tgcgccacca tttagtgttt ctagaattcc ttttttcaat tcgtcaggag
     3541 aaacctcttt aagcaaatag ccatcagcac cagcttttat agcattaaaa atattggcat
     3601 cattgtcaaa aaccgtaagc ataattatct taatatgtgg aaatttttgc ttaataattt
     3661 ctgtagcacc aatcccatcc aatttaggca tttcaatatc cattaatatc aaatcgtaat
     3721 tactgttttt ttctaattct cttattattt ctatgccatt tattgcagta cattttataa
     3781 taaaatctga aaaaagtgct agtttttgtc ttgttgcttg aataagaaat gagttgtcat
     3841 ctacaatagc taactttatc atgatttaaa atttaatttt atttgtgttc cttttgttgg
     3901 ttcagaaact atttggaatg ttcctcctat ttcttccatt cgcttttgca tattttgcaa
     3961 tccgtttcca ttagaaattg tttcaacatc aaatcctttt ccattgtctt ttataatgat
     4021 ttttatttca tttttgtcta aaataacttc tactaaaatg ttattggctt cactatattt
     4081 tatggcatta ttgatagctt cttgaataca tcgatagaca ttcattcctt gtaccgaact
     4141 aagatgtgta tttttcaagg cattgtcaac cataaaatta aaagaaatat ctgtagttgc
     4201 agttgttgcc ttttctataa aatttaaaat tcgcaatcgc aaatcttcaa aatttatggt
     4261 ttcagaattc attgcccaaa tggtatcgcg aagttctaca atagtatctt ttgtgaaatt
     4321 ggaaatattt gaaagtttgc tttctagagt agtgttttta atatcagcaa catattttgc
     4381 agtttctacg gatgagataa taaaagtcaa ttgtgcacca atattatcgt gtaaatcttt
     4441 agaaattgaa agtcgttgtt gttgtaattc attttgtttt tcaatttgag taattgccga
     4501 tttgagttca aattcttgtt tttgttgttt gttttttagt ttttgttgac ggaaaattaa
     4561 aaacccaaaa agtgcgataa aaaaaacaat tattgtcaaa cctaaaagca aaatgttttt
     4621 atatttaact tcttcttttt gttgcgcaat caatttctct tttttaacag tttgatattt
     4681 agtttcaaat tcattggttt gttttctaac agtttgttca ataattttat cttgtaaacg
     4741 cataattgca ttactataga aagaaacact gtctttttga ttaagttgtg aagatgtttg
     4801 aattaaatag ttataggttt gaattaaatc ttcaaaatag gctggctcat tagagtattc
     4861 ctttttaacg acttgagatc gggagttgtt tttgacgccg a
//
LOCUS       DAOOBH010000132         4760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_20302_length_4760_cov_6.484803, whole genome shotgun sequence.
ACCESSION   DAOOBH010000132 DAOOBH010000000
VERSION     DAOOBH010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20302_length_4760_cov_6.484803"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..558)
                     /locus_tag="PK218_10500"
     CDS             complement(193..558)
                     /locus_tag="PK218_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPW98981.1"
                     /translation="MNNFKRHNFKKLKIWQMAMELAMQIFDLTKDFPNDEKFGLISQI
                     NRCAVSIPSNVAEGSSRTNKSFSHFIDISLGSSFELQTQLILAHSKKLISIENLTYLE
                     NKLEEFQKATMTFQNTLNK"
     gene            complement(622..3636)
                     /locus_tag="PK218_10505"
     CDS             complement(622..3636)
                     /locus_tag="PK218_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW98982.1"
                     /translation="MQKKVILYFLVFILGVCQLSAQQSEIYTHNLSEFDKAIELFNNK
                     QYQSAQIIFEKVKQENKNIDTQADCAYYIATCAIRLDQSNADELMESFVSNYPTSSKQ
                     NQAYKEVAQYYFEGGKYPQALEYFNKVDESSLTYSEQEKFNFQKGYAFFTAKNKKEAT
                     AYFNKVVNSKEYGSQAKYYLGFMAYEGDNYQEANKYFDQVSTEEKYKEKLSYFKADMH
                     FKLGEFQKAIDAGTIAMAKSNAMEKSELNKIIGESYFNLKQYDKALPYLKEYKGKKGK
                     WNNTDYYQLGYTYYQQKDYENAISQFNKIIDGKDFVAQNGYYHLGESYLKLDKKQQAL
                     NAFKNASEMDFDLKIQEDANLNYAKLSYEIGNSYQSVPDVLSSFLAKYPNTPNKLEIE
                     TLLINSYITSKNYKDALVLLEKNKSTANKEAYQKVTFYRGLELYTDGNYSEALLMFKK
                     SVAEQKVAKFTARATFWKGETEYVLDNFNEALLSFKQFENYSDAKNTPEYKNINYNIA
                     YSYFKKKDYEQAGDYFQKQIDQSKSDKVRLNDAYLRLGDSRFVTSKYWPAMEAYNKAI
                     EMNGIDADYAAFQKALSYGFVSRNEKKIEDFNKFLQTFKTSQYRDDALYELANTYVNE
                     GKTDLAIKTYDQLLSESKNGTYSAKSMLKQGLVYYNNQKDDLALAKFKKVAADYPRTP
                     EAIEAVSTARLIYMDKGKVDDYASWVRTLDFIEVSDADLDNDTYEAAEKQFLLNNTKP
                     AIASLSNYVTKFPNGLHSLKANFYLAQSYYNDGLPANAVPNYEFVISKQRSEYTEQSL
                     ARLCEIHLKKEDFAKAIPVLKRLETEAEFPQNVTFAQANLMRAYFDQKDYANAVTYAD
                     KVLANPKTDDKVKSDAQIIVARSAIKANDEPKARLAYAKLLTIAKGELAAEALYYDAY
                     FKNKDGKFEDSNKAVQKLAKDYSGYKYFGSKGLILMAKNFYALKDSYQATVILDAVIK
                     NFSQFQDIVDEATQELQRIKSEEAKTNSSITN"
     gene            3823..4506
                     /locus_tag="PK218_10510"
     CDS             3823..4506
                     /locus_tag="PK218_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW98983.1"
                     /translation="MSDSVLSLKNVTIYQSNKVILSQVNLEVTKGEFLYIIGKTGTGK
                     SSFMKTLYGDLPLNEGEGNIVGYDLRKLKENDIPFLRRKIGIVFQDFQLLPDRNINEN
                     LLFVLKATGWKHMKDMQDKIDSVLNSVGMQSFANKMPHQLSGGEQQRVAIARALLNDP
                     ELILADEPTGNLDPQTSVEVMAVLKKINDQGKTIIMSTHDYALIMKFPTKTLKCEDST
                     VFEVVQKTV"
BASE COUNT         1394 a          791 c          849 g         1726 t
ORIGIN      
        1 tagataatag acagatagat gatagacgaa tagacatttt attggggatt agaccgatgg
       61 atgctagacg aatagacgtt ttattgagaa atagacagat agatgataga cgaatagacg
      121 ttttattggg gattagaccg atggatgcta gacgaataga cgaaagactt ttagttgcta
      181 taaaattcat tcttatttat ttaatgtgtt ttgaaatgtc attgttgctt tttgaaattc
      241 ttctaattta ttttctagat aagttagatt ctctattgaa attagttttt ttgaatgcgc
      301 gaggattaac tgcgtttgca attcgaaaga cgaaccgagc gatatatcta taaaatgtga
      361 gaatgattta tttgttcggc tagaaccttc ggctacattg gatggaatag aaacagcgca
      421 tctatttatt tgagaaatca acccaaactt ttcgtcgttt ggaaaatcct ttgtcaaatc
      481 aaatatttgc atggctaatt ccattgccat ttgccaaatt tttagctttt taaagttatg
      541 tcgtttaaaa ttgttcatag tttatactca atattctatc cgtctattcg tctcttatct
      601 atcatccaaa aaaaataact attaattagt gatggatgag ttggttttgg cttcttccga
      661 tttgattcgt tgcaattctt gcgtagcttc gtccacaatg tcttgaaatt gggagaagtt
      721 tttaatcacg gcatcaagaa ttacggtggc ttgatagcta tcttttagcg cgtagaagtt
      781 ttttgccatc aaaatcaatc cttttgaacc aaaatattta tatcccgaat agtctttggc
      841 caatttctgc acggctttgt tggaatcttc aaactttccg tctttgtttt tgaaataggc
      901 atcataatat agtgcttcgg ctgccaattc tcctttggcg atggtcaaca atttggcata
      961 agcaagtcta gcctttggtt cgtcgttggc ttttatagcc gaacgcgcca cgatgatttg
     1021 agcatcgctc tttactttgt cgtctgtttt tgggtttgcc aaaactttgt cggcataagt
     1081 cacggcgttt gcataatcct tttggtcaaa gtaggcacgc atcaagttgg cttgtgcaaa
     1141 ggttacgttt tgtggaaatt ctgcttcggt ttccaagcgt ttcaacaccg gaattgcctt
     1201 tgcaaagtct tcttttttca ggtggatttc gcatagacgt gcaagcgatt gttcggtata
     1261 ttcgcttctt tgtttgctga tgacgaactc ataatttgga acggcgttgg ctggcaaccc
     1321 atcattatag tatgactgtg ccaaatagaa gttggctttc aaggaatgca agccgtttgg
     1381 aaacttcgtt acataattgc tcaatgaagc aatggcaggc ttggtattgt ttaacaaaaa
     1441 ttgtttctcg gcagcttcat acgtgtcgtt atctaggtcg gcatccgaaa cttctatgaa
     1501 atctagagtt ctaacccacg aagcgtagtc gtcaactttg cctttgtcca tataaatcag
     1561 cctagcagtt gaaacggctt cgatggcttc gggtgttctt gggtaatctg cagctacttt
     1621 tttgaacttg gctaacgcca aatcgtcttt ttggttgttg taatacacca aaccttgttt
     1681 gagcatcgat tttgccgaat aggttccgtt ctttgattcg ctcaacagct ggtcgtaggt
     1741 cttgattgct aggtctgttt ttccttcgtt gacataagtg tttgctaatt cgtacaaagc
     1801 atcatcacga tactgagacg ttttgaatgt ttgcaagaac ttattaaaat cctctatttt
     1861 tttctcattt cttgatacga aaccgtagct caatgccttt tgaaacgctg cataatcggc
     1921 gtcaatgcca ttcatttcga tagctttgtt gtaggcttcc atggctggcc agtattttga
     1981 agtaacaaaa cggctgtcgc ctaatcttag ataagcatcg ttcaaacgta ctttatcgct
     2041 ttttgattgg tcaatttgtt tctggaaata atcgcctgct tgttcgtagt ctttcttttt
     2101 aaaataggaa tacgcaatgt tgtaattgat gtttttgtat tctggagtgt ttttggcatc
     2161 cgaataattc tcaaactgct tgaaacttag caaagcttcg ttaaaattat ctaagacata
     2221 ctcggtttct cctttccaaa aggttgctct tgcggtaaac ttggctactt tttgctctgc
     2281 cacagacttt ttgaacataa gtaaagcttc ggaatagttg ccatcggtgt ataattctaa
     2341 acctcgataa aaagttacct tttgataggc ttctttattg gcggttgatt tgtttttttc
     2401 tagtaaaacc aatgcgtctt tgtagttttt agaggtgata taggaattaa tcaacaacgt
     2461 ttctatctct agtttgtttg gcgtattagg atattttgct aaaaaggaag acaaaacatc
     2521 aggcacactt tggtacgagt ttcctatttc gtaactcaac ttggcgtaat tcaaattcgc
     2581 atcttcttga atctttaaat caaaatccat ctcagaagcg ttcttaaaag cgtttaaggc
     2641 ttgttgtttc ttgtctaatt ttaaataact ttcacccaaa tgatagtaac cgttttgagc
     2701 cacaaagtct tttccgtcaa tgattttatt gaattgcgaa atcgcattct cataatcttt
     2761 ctgctgataa taagtgtacc ctaattggta gtaatcggtg ttgttccatt ttcctttttt
     2821 gcctttgtat tcttttaaat agggcaatgc tttgtcgtat tgctttaaat tgaaatagct
     2881 ttcgccaata attttattca attcggattt ctccattgcg tttgattttg ccatagcaat
     2941 agttcctgca tcaattgctt tttggaactc gcccaatttg aaatgcatgt cggctttgaa
     3001 gtaagacaac ttctctttgt atttttcttc ggtagaaact tggtcaaaat acttgttggc
     3061 ttcttgataa ttatcgcctt cgtacgccat aaaccctaaa taatacttgg cttgcgagcc
     3121 gtattctttg gaattcacga ctttattgaa gtaggctgta gcttcttttt tgtttttagc
     3181 ggtgaaaaaa gcatatcctt tttggaaatt aaacttttct tgctcactat aagtcaatga
     3241 actttcatct actttattga aatattctaa ggcttgtgga tattttccgc cttcaaaata
     3301 atattgcgct acttctttat aggcttgatt ttgcttagaa cttgttggat aattagaaac
     3361 aaaactttcc atcaactcat ccgcattcga ttggtctaaa cgaatagcac acgttgcaat
     3421 gtagtaagca caatcggctt gtgtatcaat attcttgttt tcctgtttga ctttttcaaa
     3481 aataatttgt gctgattgat actgtttatt attgaacaac tcaatggctt tgtcaaattc
     3541 acttaggtta tgtgtgtaaa tttcagactg ttgtgccgac aattgacaaa cacccaaaat
     3601 aaatactagg aaataaagga ttactttttt ttgcattgct tataatttta cttttttcaa
     3661 aaatagaact ctatatacta taacgtaaaa acataccatt ttattataaa cattctttta
     3721 aacaattcat tttttaactt tttgatagga attttagtta taaaaaagat tttggagttt
     3781 ggtagtaata tttattactt ttaccaaata aaattcttca aaatgtctga ttcagtttta
     3841 tcgttaaaaa atgttaccat ttatcaaagc aacaaagtta ttttgtcgca agtaaatctt
     3901 gaagtaacca aaggcgaatt tctttatatt attggaaaaa ccggaactgg aaaaagtagt
     3961 tttatgaaaa ctttgtatgg cgatttgcct ttaaacgaag gcgaaggaaa cattgttggc
     4021 tatgatttga gaaagctaaa agagaatgat attccttttt tgagaagaaa aattgggatt
     4081 gtttttcaag attttcaatt attacccgat agaaacatta atgaaaatct attgtttgta
     4141 ttgaaagcaa ctggctggaa acatatgaaa gacatgcaag ataaaataga cagcgtgctg
     4201 aatagtgttg gaatgcaatc ttttgccaac aaaatgcctc atcaactctc tggcggcgaa
     4261 caacaacgtg ttgccattgc tagagcttta ttgaatgatc ctgaattgat attggcagat
     4321 gaaccaactg gaaatcttga cccacaaaca agtgttgaag taatggcggt tttgaaaaaa
     4381 ataaacgatc aaggaaaaac gattattatg tcaacacatg attatgcatt aatcatgaaa
     4441 ttcccaacga agacattgaa atgcgaagac agtactgttt ttgaagtagt tcaaaaaacg
     4501 gtttaaacta tcaaaaatat tcatttaatt taaaaaatta gagcaataaa ttaatcgctt
     4561 ttttggtgaa gctcaataat ttttatacca tttactaaca tcatttttct gaataaggaa
     4621 aatccattga acttactaaa gaaaaatcaa tgatgtaatt aagtttaatc aattctggtt
     4681 tttttgaatt acctagtaac gattttttat ttgaaattaa gaaaaattaa aatcatttct
     4741 agtaataaca caaattttat
//
LOCUS       DAOOBH010000133         4643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_20934_length_4643_cov_4.423932, whole genome shotgun sequence.
ACCESSION   DAOOBH010000133 DAOOBH010000000
VERSION     DAOOBH010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20934_length_4643_cov_4.423932"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PK218_10515"
     CDS             <1..426
                     /locus_tag="PK218_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW98984.1"
                     /translation="AGNYTIAIDELDGIFSGNQNVYLRDNFLGIEHDIKSSAYNFITN
                     VGTFDNRFEIVYRITTLGFSEVKDNTTFGMINDQLLTINSSEIIKSITVFDIAGKMIL
                     NSDTVSAKQFTQSFNHPNGVYIAKVKLNNGFVANVKLIK"
     gene            complement(518..2668)
                     /locus_tag="PK218_10520"
     CDS             complement(518..2668)
                     /locus_tag="PK218_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPW98985.1"
                     /translation="MKKIILSFIAALLLVPTSVKADEGMWFLMFIERLNHRDMQKMGL
                     QLTAEEIYSINHHSLKDAIVQFNGGCTAEIVSKDGLVLTNHHCGYDAIAELSSEEQNY
                     LKNGFWAKNRKDEMKPSSLYVRFFVRMDDVSKRILSKVNDKMSEADREKAINQEIAAI
                     EKENNEGGKYTVSVRPFFQGNEYYYFVYQDYKDVRLVGTPPESLGKFGGDTDNWEWPR
                     HTADFSMFRVYADANGNPADYSPENVPLQPKHYLPVNIGGVQEKDFAMILGYPGRTNR
                     WMPAGGIEQNVKFAYPAWVEGAKVGMDNMKKYMNESEAINLKYASKFARTANYWKNRQ
                     GMIDALTKFGTAKSKASQEAKFDKWANKSANKSKYGHVISDINNFYALTNEKSKHDNY
                     MQQLFRTSSFGSISRSMGRYLDAYVKGDAVKRAQIAPAIEEMANEMFKEIYIPAEKDI
                     LAAQLNTYATKATAYGIAPMVKQIGDANNNDFSKYVNAAFDLSILTSVEKIKAFLEIP
                     SQEMLDNDPIYKLSSDLMTHYYKKSDEIAAAEDKFSAAFRLLVEGLRESKIGTIKYPD
                     ANSTLRLTYGKVRALPADKRNDATINNYTTLAGQVKKYKKGDAEFDLPTRVLEMQKNK
                     DYGRFADKDGSLHVNFLTDNDITGGNSGSPVLNGKGELIGLAFDGNIEAMAGDVIFDK
                     NLQRTINVDIRYVLWVIENFSGAKHIVDEMTLIK"
     gene            2893..3357
                     /locus_tag="PK218_10525"
     CDS             2893..3357
                     /locus_tag="PK218_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98986.1"
                     /translation="MKYIIIPIIALGFTLCSLFGVEYSCIGPEMFPHFYGSPFVYMQN
                     NLGSSMEYFYSISGLVLNVIVWSIPLASIHCLLEYLINRTPKPKLARVFYYSVVDVLV
                     FFSFLNLNMAYITLGRGFSENTNYWYWNMDKESASWGMKCHGEWSFIQFYYQ"
     gene            3419..3913
                     /locus_tag="PK218_10530"
     CDS             3419..3913
                     /locus_tag="PK218_10530"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW98987.1"
                     /translation="MLSKNSLCVLTLLLISTITHAQKEKTEYTPVTPPDLVVKETAIE
                     YNEPHPFMVLDEIPQFKECKEVSKEEAKACFEEQLKKHIRKNLKYPAEAKKNNIQAKV
                     IVQFIINQEGKVTDLRAKSPKGAELLEAEAIRVIEKLSKFIPGKQRGRVVNTSYAVPI
                     LFKM"
     gene            4142..4267
                     /locus_tag="PK218_10535"
     CDS             4142..4267
                     /locus_tag="PK218_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98988.1"
                     /translation="MYKIESKENDVFEYEMTLHEDGTFDLYYHSYINRGILPEKD"
BASE COUNT         1452 a          865 c          766 g         1560 t
ORIGIN      
        1 gccggaaact atactatcgc tattgatgaa ttggacggta ttttctctgg caatcaaaat
       61 gtgtacttaa gagacaattt tcttgggatt gaacacgata taaaatcaag tgcttacaac
      121 tttattacca atgttggaac gtttgacaat cgatttgaaa ttgtttatag aataactact
      181 ttgggctttt ctgaagtgaa agacaacact actttcggaa tgattaatga ccaattacta
      241 accatcaact caagtgaaat tattaaaagt attacggttt ttgacatagc tggaaaaatg
      301 attttaaaca gtgatactgt atcggcaaaa caatttacac aatcctttaa ccatccaaat
      361 ggtgtttata ttgcaaaagt aaaactaaac aatggttttg tggctaatgt gaagttgatt
      421 aaataattcg cttattatta aaccaaaaaa aactccggtt gataggtcaa ccggagtttt
      481 tttttgataa tccttcttag gggattgaat gtctagttta ttttattaac gtcatttcgt
      541 caacaatgtg ttttgcacca gagaaattct caattaccca aagtacataa cgaatatcta
      601 cgttgatggt tctttgtagg tttttgtcaa aaataacatc accagccata gcttcaatgt
      661 taccatcaaa tgctaaaccg attagttctc ctttaccatt taatactggc gaacctgaat
      721 ttccacctgt gatgtcatta tccgtaagga aatttacgtg taacgaacca tctttatcag
      781 caaatctacc gtagtctttg tttttttgca tttcaagaac acgagttggt aaatcaaatt
      841 cggcatcgcc ttttttatat ttcttaacct gacctgcaag tgttgtataa ttattaatgg
      901 tagcatcatt gcgcttatct gctggcaaag cgcgaacttt accataggtt aatcgcaatg
      961 ttgagttagc atctggatac ttgattgtac caatttttga ttctctcaaa ccttctacca
     1021 acaaacggaa agctgcacta aatttatctt ctgctgctgc aatttcatcc gattttttat
     1081 aatagtgcgt catcaaatct gaagacaatt tgtatattgg atcattgtcc aacatttctt
     1141 gacttgggat ttctaagaat gctttgattt tttctaccga tgttaagatg ctcaaatcaa
     1201 aagctgcgtt tacatattta gaaaaatcat tgttgttagc atcaccaatt tgcttcacca
     1261 ttggagcaat tccataggca gttgctttgg ttgcgtaagt gtttaattgt gccgctaaga
     1321 tgtctttttc ggcaggaata tatatttctt taaacatctc gtttgccatt tcttctatag
     1381 ctggggcaat ttgtgcgcgt tttacagcat ctccttttac ataagcatct aaatatcttc
     1441 ccatcgaacg acttatagaa ccaaaagaac ttgttctaaa caattgttgc atgtaattgt
     1501 catgttttga cttttcatta gtcaacgcat agaagttatt gatgtctgaa attacgtgtc
     1561 catattttga tttgttggct gatttgtttg cccatttgtc aaatttagct tcttgcgatg
     1621 cttttgattt tgcggttcca aattttgtta aagcatctat cattccctga cggtttttcc
     1681 aataattggc cgttctggca aacttagaag catattttaa gttgattgct tcgctttcgt
     1741 tcatgtattt tttcatgttg tccataccta ctttggcacc ttcaacccat gcaggataag
     1801 caaacttaac attttgttct ataccacctg ctggcatcca acggttggtt cttcccggat
     1861 aaccaagaat cattgcaaaa tctttctctt gaacacctcc aatatttact ggtaaatagt
     1921 gctttggttg caacggaaca ttctctggag aatagtctgc tggattacca tttgcatctg
     1981 catatactct aaacattgag aaatcggcag tgtgacgtgg ccattcccaa ttgtcagtgt
     2041 ctccaccaaa ttttcctaag ctttctggcg gtgttcctac taaacgaaca tctttgtaat
     2101 cttgatatac aaagtagtag tactcatttc cttgaaaaaa aggacgtacc gaaacggtat
     2161 attttccgcc ttcgttgttc tctttctcaa tagccgcaat ttcttggttg atggctttct
     2221 ctctatcagc ttcagacatt ttgtcgttta ctttagaaag tattctttta gaaacatcgt
     2281 ccatgcgaac aaaaaatcga acgtataacg acgatggttt catctcgtct tttctgtttt
     2341 ttgcccaaaa accatttttc aaatagttct gctcttcaga tgataattcc gcaatggcat
     2401 cgtagccaca gtggtgattt gttaaaacca aaccgtcttt tgaaacgatt tcggcagtac
     2461 aacctccgtt gaattgtaca atagcatctt tcaaactatg atgattgatg ctgtaaattt
     2521 cttcggctgt cagttgcaag cccatttttt gcatgtcgcg gtggtttaat ctttcgatga
     2581 acatcaaaaa ccacattcct tcgtcagctt ttacacttgt tggcacaagt agcaacgccg
     2641 cgataaagga taaaataatt tttttcattg taattaaaat aagtgttatt agtttactat
     2701 agtagtagtc gttgttattt caaatttgtt acaaacctct ccacaccaac aaacagttgg
     2761 cgaatatact tttttttgag taaagataaa agcattaaca tcttagcatt ctcttaaaga
     2821 aacagatttt tagtcctcca cccttttatt tccaaaaatt tcatacattc gtgattgtca
     2881 attatcaaac taatgaaata catcatcatc ccaatcattg cattaggatt tactttgtgt
     2941 tccctttttg gggtggaata tagttgcata ggacccgaaa tgtttcctca tttctatgga
     3001 agtccttttg tgtatatgca aaataactta ggcagctcta tggaatattt ctatagcatt
     3061 tcgggattgg tattgaacgt aattgtttgg agtatcccac tcgctagtat ccattgcttg
     3121 ctcgaatatt tgataaaccg cacaccgaaa ccaaaattgg cgcgcgtttt ttattattcc
     3181 gtagttgatg ttcttgtgtt tttttcgttc ctaaatctaa acatggccta cataactttg
     3241 ggacgcggat ttagtgaaaa caccaattat tggtattgga acatggacaa agaatcagcc
     3301 agttggggaa tgaaatgtca tggcgaatgg tcgtttattc aattctacta tcaataatat
     3361 tagcctaaaa aacagtaact ttgccacccc taattttcga caacgctatt ttatatctat
     3421 gctttcaaaa aacagcctct gtgtcttaac cctgctttta atcagcacaa ttacgcatgc
     3481 tcaaaaagaa aaaacggaat acacacctgt tactccgccc gatttggttg ttaaagaaac
     3541 agctattgaa tataacgaac ctcatccgtt tatggtttta gacgagattc ctcagtttaa
     3601 agaatgtaag gaggtgtcaa aagaagaagc taaagcttgt tttgaagaac aattgaagaa
     3661 acacatcaga aaaaatctaa aatacccagc agaggctaaa aagaacaaca tccaagctaa
     3721 agttattgtt caattcatca taaaccaaga aggaaaagtg accgatttga gagcaaaaag
     3781 cccaaaaggt gctgagttac tagaagccga agccattagg gttatcgaaa aactatcaaa
     3841 atttattccc ggaaaacaac gaggtagggt tgtaaacaca tcctatgctg ttccaatcct
     3901 ttttaagatg taaggactca gtaacaatct atatcaaaag atgattttgt tctttggaca
     3961 tttcatcaca cattttaaaa gagactagaa ttagcaacta tataattcaa attaaaaaaa
     4021 attaccaaca actaataaaa aaaaaatcaa ctcaataaaa aaaataattg tctttaccgt
     4081 atttcttttt gtctctagcc tatctttgtt ttcgcaatcg gaaaaagtgg cgggcaagta
     4141 catgtataaa attgaatcta aagaaaatga cgtatttgaa tatgaaatga cgctgcacga
     4201 agacggaacg tttgatttgt attatcactc atacatcaat cgaggcatcc tgccagagaa
     4261 agactaatca gacaaaaaca tggtgtagaa aactttgcag cttccttcac caacgttgcc
     4321 cagaaagata tagtcgaaaa atatacgtta gacttcacca acactaaagc acgctttgtt
     4381 accaaatcag ttagagacaa aaccgataaa gttgtagcaa ccaaacttca tttcttaaag
     4441 tcggatatta cttggataag cagaattgat ttgcttaaga attgacttgt cctaaataat
     4501 ctgtatcgat tatttaggac aagtcacata aaaaaaacgg aacagaataa attctgttcc
     4561 gttttctatt atagaataat taaagtttaa ttatcttttt acaactctaa cggttcgtgt
     4621 ttcgtcagct tgtgttacaa tta
//
LOCUS       DAOOBH010000134         4579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_21270_length_4579_cov_8.359416, whole genome shotgun sequence.
ACCESSION   DAOOBH010000134 DAOOBH010000000
VERSION     DAOOBH010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21270_length_4579_cov_8.359416"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..3221
                     /locus_tag="PK218_10540"
     CDS             126..3221
                     /locus_tag="PK218_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPW98989.1"
                     /translation="MSSENPILNSPYDEPKLHYATDADGSLNYSDIREGRRIFSPDIQ
                     VIPTRQGQQGSIFEVNDFAAEYGTHIINLCRKEVGKWREEKYPNTTRVTKELLLFWFL
                     NPDRHATKQLFFAQREAVETAIWLNEVAEKSNAGQNILNKIREGQQTVSEDSADQLPR
                     IAYKMATGSGKTVVMACFILYHYFNRQEYRNDTRFADYFLIVAPGVTIKDRLGVLFVD
                     TKTKNPAEIQDYYHVRALVPQNMEHRLDNLNARLVITNYHTFEPKTLKGNKRSPFDGK
                     VDLEGNKIDTGNLEDFSQVIKRTLSKFKSGTRLLVMNDEAHHCYLPKSKGKTSDNEEA
                     DENQRAAVWFTGLKEISKRFKLQNIYDLSATPYYLTGSGYTPYSLFPWVVSDFGLIEA
                     IESGLVKIPFLPESDNTQELTMPVLRNLYEHVKDELPRKGQRKQKSEAAKEGQKIKEQ
                     PPRLPALVKAALDQFYGHYVEYYNGVRKNQEQKANLFSSPPVFILVCNNTSVSKEVYK
                     YIAGYEYLDENEKLVTVSGAKELFSNYDPVTKKPLKRPPTLLIDSDALDNSDQINEDF
                     KKIFASEIEEFKKDYARLHGQGSAENISDGEILREVVNTVGKQNKLGSHIRCVVSVSM
                     LTEGWDANTVTHIMGLRAFGSQLLCEQVAGRALRRMNYFLQGYDKDGNPTEDKRKIVV
                     YKFPPEYAHIIGVPFKMFKGGKTEPPPQPVDLTHIAALPERQEKMEIEFPNVVGYRIE
                     NYDGEIKYDFSNIENYEIRGDITPIETQLASAFSKEEIQIKIQSVLEKRDQEVIFWLT
                     KELIKFHFSDDEGSPNFQKFNKLKSIVAEWYDKKVILLNIQDQRYKRLLFFEEPKKMV
                     DHIARGINPHINTTEHVRPVFNYYNKFSSTKYVNGNTIKEVYATQKSHVNYVVMDSDW
                     EGIAAKTLEELPQVISYVKNQFLGFAIPYVKEGKDKNYFTDFIVQCKGKDGTIKNLMI
                     EISGFSKDKAEKKWFVENRWIPAVNALKDKYEYPEWHFIEIANDIRNIKNQLIEKISS
                     I"
     gene            3225..>4579
                     /locus_tag="PK218_10545"
     CDS             3225..>4579
                     /locus_tag="PK218_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPW98990.1"
                     /translation="MAKKIDSIKHSKDTRVHIPSKEEAGYEEANSKVQSGKKILEIPK
                     NPVVHRGQDPELFWLNKYGNDDRDDLLRVDIRSLYRHEHIAPETLIKNLYKLTETKID
                     QLDLFSINELFGNALEKDELEKVGEYYHHQDGWTNRLIQGDSHLVMASLLEREGMAGQ
                     VQMMYFDPPYGINYSSNWQVKITNTNVKESSDDDLSGEPEVIKAFRDTWEYGIHSYLS
                     YLRDRLLIALELLHETGSCFVQISSQNVHLVRSLMDEVFGSDNFVSQIAYRTKNMTID
                     TKLLGSVNDFIVWYAKDKEKVKYRKLYQNTTVEGDSHWNLYQLPDGSVHKMTSDEINN
                     HKLLPKGADPCQLIALYPIGYSPDRDFEVTFKGKKYKPANNRSWKFDADGFKKLVAAN
                     RIVPYSSGDTIRYLYKLSDYPVSPMHSLWHETAPASSKEYVVQTSTTPIQRCIQMTTD
                     PG"
BASE COUNT         1719 a          747 c          832 g         1281 t
ORIGIN      
        1 cacaaatggc acatttgctt ttgccccgac acacgaagcc aacccttcgg caaaatcaaa
       61 agagccattt tttgccatcg caccaagaaa aatatgtact ttgcatttaa aatattagac
      121 ttaaaatgag ttcagaaaat ccgattttaa acagcccata cgatgaacca aaactgcatt
      181 atgcgacaga tgcggatggt tctttgaatt atagcgacat cagagaaggt aggagaatat
      241 tctcaccaga cattcaagtt atcccaacaa gacaaggaca acaaggttct atttttgaag
      301 taaatgattt tgctgctgaa tatggcactc acataattaa tctttgtcgc aaagaagttg
      361 gaaaatggag agaagaaaaa tatccaaata ctactagagt aacaaaagaa ctgttacttt
      421 tttggtttct aaatcctgac agacacgcaa caaaacaatt attctttgca caaagagaag
      481 ctgttgaaac tgcaatctgg ttaaatgaag tcgctgaaaa atcaaatgca gggcaaaaca
      541 tattaaataa aataagagaa gggcaacaaa ctgtaagtga agactcagca gaccaattgc
      601 cgagaattgc ttataaaatg gcaacaggtt caggtaaaac tgttgtaatg gcttgtttca
      661 ttttatatca ttatttcaac agacaagaat atagaaacga tactcgtttt gctgactact
      721 tcttaatagt tgcacctgga gttacaatca aagaccgatt aggagtttta tttgttgaca
      781 caaaaacaaa aaatccagca gaaattcaag attactatca tgtaagggct cttgttcctc
      841 aaaacatgga gcatcgatta gacaatttaa atgcaagatt agttattaca aattatcaca
      901 cctttgaacc aaaaacctta aaaggaaata aaaggagtcc atttgatggt aaagtagatt
      961 tagaaggaaa taaaattgat actggaaacc ttgaagattt ttctcaagtt ataaaacgaa
     1021 ctttaagcaa atttaagtca ggtactcgtt tgcttgtaat gaatgatgaa gcacaccatt
     1081 gctatttgcc taaatcaaaa ggaaaaacct cagacaatga agaagcagac gagaaccaaa
     1141 gagcagctgt ttggtttaca ggattaaaag aaatctcaaa gcgttttaaa cttcaaaata
     1201 tttatgacct ttccgctaca ccgtattatt taactggttc gggatatact ccttacagtc
     1261 tttttccttg ggtcgtatct gattttggct taattgaagc aattgaatca ggtttagtaa
     1321 aaattccatt tttgcccgaa agtgataata cgcaagaatt aacaatgcct gttctgagaa
     1381 atttatacga gcacgtaaag gatgaattgc caagaaaagg tcaacgaaaa caaaaatcag
     1441 aagctgctaa agaaggtcaa aaaattaaag aacagccgcc aagattacca gcattagtga
     1501 aagcagctct cgaccaattc tacggacact atgttgaata ttacaacgga gtaagaaaga
     1561 atcaggaaca aaaggcaaat cttttttctt ctccaccggt ttttatttta gtctgcaata
     1621 acacatcagt ttctaaagaa gtttacaaat acattgctgg atatgaatat ttggatgaaa
     1681 atgaaaaatt agtaactgtt tctggagcaa aagaattatt cagcaattat gaccctgtta
     1741 caaagaaacc actaaaaaga ccgcccaccc tattaattga cagtgatgca ttagacaata
     1801 gcgaccagat aaatgaagat ttcaagaaaa tatttgcttc cgaaatagaa gaatttaaaa
     1861 aagattatgc acgcctacat ggacaaggaa gtgcggaaaa catttcagat ggtgaaattt
     1921 tacgtgaagt agtaaatact gttggcaaac aaaataaatt gggttctcat attcgttgcg
     1981 ttgtttctgt ttcaatgctt actgaaggtt gggacgctaa tacagttact catattatgg
     2041 gtttacgtgc gtttggttct caactacttt gcgaacaggt tgcaggtcgt gctttgcgaa
     2101 gaatgaatta ttttttacag ggctatgaca aagatggaaa tccaacggaa gacaaaagaa
     2161 aaattgttgt gtataaattt ccacctgaat atgcacacat tattggagta ccatttaaaa
     2221 tgtttaaagg tggaaaaacc gagccaccac cacaacctgt tgatttaaca catattgcgg
     2281 cattacctga acgacaggag aaaatggaaa tagaatttcc taatgttgta ggttaccgga
     2341 ttgagaacta cgatggtgaa ataaaatatg atttcagcaa tattgaaaac tacgaaatac
     2401 gtggtgatat tacacctatt gaaactcaac tagcaagtgc cttttcaaaa gaagaaatac
     2461 aaattaaaat acaatctgta cttgaaaaaa gagaccaaga agttattttt tggctaacaa
     2521 aagaacttat taaatttcat ttctccgatg atgaaggaag cccgaacttt caaaagttta
     2581 acaagttgaa atccattgta gcagaatggt acgataaaaa agtaatacta ctaaacattc
     2641 aagaccaacg atataaaaga ctattatttt ttgaagaacc taaaaaaatg gttgaccata
     2701 ttgcaagagg cataaatccg catatcaaca caacagaaca tgtacgacct gtttttaact
     2761 attacaacaa atttagcagt acaaaatatg tgaacggcaa tactataaaa gaagtttatg
     2821 ccacacaaaa aagtcatgta aattatgttg taatggatag cgactgggaa ggtattgcag
     2881 ctaaaaccct tgaagaattg ccccaagtaa ttagttatgt caaaaatcaa tttttgggtt
     2941 ttgcaatccc ttatgtgaaa gaaggtaaag acaaaaatta tttcacagac tttattgtgc
     3001 aatgcaaagg caaggacggt actattaaaa atctgatgat tgagataagc ggttttagta
     3061 aggataaagc cgaaaaaaaa tggtttgttg aaaatcgttg gattccagct gtaaatgcac
     3121 tcaaagataa atacgaatat cctgaatggc atttcattga aattgctaat gacattcgca
     3181 atataaagaa tcaactaatt gaaaaaattt cgagtattta agatatggca aagaaaatag
     3241 acagcataaa gcacagcaaa gatacacggg ttcatatacc aagtaaggaa gaagccggtt
     3301 acgaagaagc aaactctaaa gtacaatccg gtaaaaaaat attggaaatt cccaaaaacc
     3361 ccgttgtaca tcgtggacaa gaccccgagt tattttggct taataaatac ggaaatgacg
     3421 accgtgatga tttattacgg gttgatataa ggagtttgta ccgccatgag catattgcac
     3481 cagaaacgct tattaaaaac ctttacaagc taacagaaac aaaaatcgac cagttagatt
     3541 tgttcagtat taatgaactt tttggtaatg cattagaaaa agacgaactc gaaaaagtag
     3601 gtgaatatta tcaccatcag gacggctgga caaaccgcct gatacaaggc gacagccatt
     3661 tggttatggc aagtttgctg gaacgcgaag gaatggcagg tcaggttcaa atgatgtatt
     3721 ttgacccacc ttatggtata aattacagtt caaattggca ggtgaaaatt accaatacga
     3781 atgttaaaga aagtagcgat gatgatttgt ctggtgaacc cgaagttata aaagcttttc
     3841 gtgatacatg ggaatatggt attcactcat atttgagtta tttgcgagat agattattaa
     3901 ttgctttgga attgcttcac gaaacaggtt cttgctttgt tcaaatctca tcacaaaatg
     3961 tccatttagt aaggtcatta atggatgaag tttttggcag tgataatttt gttagccaaa
     4021 tagcatatag aaccaagaat atgacaattg atacaaaact tttaggttca gtaaatgatt
     4081 ttattgtttg gtatgcaaaa gataaagaaa aagtaaaata ccgcaaatta taccaaaaca
     4141 caacagttga aggtgatagt cactggaatt tatatcaatt acctgatggt tctgttcata
     4201 aaatgacttc tgatgaaata aataatcata agttattacc caaaggagca gacccctgcc
     4261 aactaattgc cctttatcct attggttata gtcctgatag agattttgaa gttacgttta
     4321 aaggcaaaaa atataaacca gctaataatc gctcatggaa atttgatgct gatggtttta
     4381 aaaaacttgt tgctgcaaat agaattgttc cttattcatc tggtgataca ataagatatt
     4441 tatacaaatt atcagactat cctgtttcac ccatgcattc attatggcat gaaacagcac
     4501 cagcatcatc caaagaatat gttgttcaaa catcaaccac gccaatacaa cgttgtattc
     4561 aaatgacaac tgacccagg
//
LOCUS       DAOOBH010000135         4374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_22534_length_4374_cov_5.834684, whole genome shotgun sequence.
ACCESSION   DAOOBH010000135 DAOOBH010000000
VERSION     DAOOBH010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22534_length_4374_cov_5.834684"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PK218_10550"
     CDS             <1..1236
                     /locus_tag="PK218_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HPW98991.1"
                     /translation="MKLIAYLKELLASKEMRMQVIKDELIEIRDKYGDERRSQIEYSG
                     GDVSIEDLIADENVVITISHAGYIKRTNLSEYKTQNRGGVGQKSAGTRDQDFLEHLFV
                     ATNHQYLMFFTQKGKCFWMRVYEIPEGSKTSKGRAIQNLINIESDDKVKAFICTQDLK
                     DEEYINNHYVIMATKQGQVKKTSLEQYSRPRVNGINAITIREDDELLEAKLTNGESQV
                     LIAVKSGKLVRFEEEKTRPMGRTASGVRGITLANDKDEVIGMVSVNKDDITDTQLLVV
                     TENGYGKRTKLVDDDGEDVYRITNRGGKGVKTLNITEKTGALISINAVTDEDDLMIIN
                     KSGLTIRMEVSDLRVMGRATQGVRLINIKGNDSIAAVTKVMKEDEIEETELEGTEINE
                     NDVESSQNETSTENTETED"
     gene            1253..2572
                     /locus_tag="PK218_10555"
     CDS             1253..2572
                     /locus_tag="PK218_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW98992.1"
                     /translation="MKIRQIILASALLVSVSNFAQKDELKKLKKIYDKDVPSAKEVEE
                     YKMNVASLEKVASDEADKMALNYYKVNIPQMELASKGQVPNMMDLQRVYTPKYISELA
                     KTYSDVIDFEKKSGKKTFTDDINEEFAQSKPIILNLAIEMGNQKKFKESASIFHSLYL
                     MDKKDVDQLYYAASYATNGQDYDTALEYYNELIRLNYIGDGMIYYAVNKTNKQEESFG
                     NNKSTRDTFVKVGTYEKPRDEKIESKKAEIYKNVALILVQQGKTDEAMKAVSQARIEN
                     PDDANLILTEADLYLKLKDITTYKKLINEALDKDPNNADLVYNLGVISSQADQLEDAI
                     KYYKKAISIKPDYFNAYLNLSEVMLRGDEKYVEEMNKLTTSDKDNKRYAVIKAEREKN
                     FQAVLPYLEKAVELKPDNEPAAKTLMSVYNALEMTDKYKALKAKMNN"
     gene            complement(2637..4025)
                     /gene="lpdA"
                     /locus_tag="PK218_10560"
     CDS             complement(2637..4025)
                     /gene="lpdA"
                     /locus_tag="PK218_10560"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPW98993.1"
                     /translation="MKYDIIVLGSGPGGYVTAIRASQLGFKVAIIEKENLGGICLNWG
                     CIPTKALLKSAQVFDYLKHASDYGLTVKEFDKDFSAVVKRSRDVADGMSKGVQFLMKK
                     NKIDVIDGFGKIKPGKKIDVTAADGKVTEYSADHIIIATGARSRELPNLPQDGKKVIG
                     YRQAMTLPEQPKSMIVVGSGAIGVEFAHFYNSMGTQVTIVEFMPNVVPVEDEDVSKQM
                     ERSMKKAGITIMTSSSVEKIDTSGNGVKAYVKTAKGEEIIEADILLSAVGIKTNIENI
                     GLEEVGIATDKDKILVNAYNQTNVPGYYAIGDVTPGQALAHVASAEGINCVEKIAGMH
                     VDPIDYGNVPGCTYATPEIASVGLTEKQAKEKGYELKIGKFPFSASGKASASGAKDGF
                     VKVIFDAKYGEWLGCHMIGAGVTDMIAEAVVARKLETTGHEILKAIHPHPTMSEAVME
                     AVADAYGEVIHL"
     gene            complement(4070..>4374)
                     /locus_tag="PK218_10565"
     CDS             complement(4070..>4374)
                     /locus_tag="PK218_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98994.1"
                     /translation="QNETHKITDALEELKLLCKENNILLICIQTPVYKSTYDTKAFEI
                     PKTICKKLNIPFIDLSNSYIKDKMNYFYNDIHLNKNGVTEMNKLLKNEKELTNFLK"
BASE COUNT         1598 a          643 c          745 g         1388 t
ORIGIN      
        1 atgaaattga ttgcgtattt aaaagaatta cttgcaagta aagaaatgag aatgcaagtt
       61 atcaaagatg aattaattga gattagagat aaatacggag atgagcgtcg ttctcaaatc
      121 gaatactctg gtggcgatgt tagtattgaa gatttaattg ctgatgaaaa tgttgtaatt
      181 acaatttctc acgctggata cattaagcga actaatttat cagaatacaa aactcaaaac
      241 agaggtggtg ttggacaaaa aagtgcagga acaagagatc aagatttctt agaacattta
      301 tttgttgcta caaatcacca atacttgatg ttctttacac aaaaaggaaa atgtttttgg
      361 atgcgtgtat atgaaattcc agaaggaagc aaaacgagca aaggaagagc gattcaaaat
      421 ttaattaata ttgaaagtga tgataaagta aaagcattca tttgtactca agatttaaaa
      481 gacgaagaat acatcaataa tcattatgta atcatggcga ccaaacaagg tcaagtgaaa
      541 aaaacatctt tagaacaata ttcacgtcca agagttaatg gaattaatgc aataacaata
      601 cgtgaagacg acgaattact agaagcaaaa ttaactaatg gagaaagcca agtattgatt
      661 gcagttaaat ctggaaaatt ggttcgtttt gaagaagaaa aaacacgacc aatgggaaga
      721 acagcttctg gagttcgtgg aattacttta gcaaatgata aagacgaagt gattggcatg
      781 gtttctgtta ataaagatga cattacagac acacaattat tagtagtaac cgaaaatggt
      841 tatggaaaaa gaacaaaatt agttgatgac gatggagaag acgtttatcg aattactaat
      901 agaggtggaa aaggtgtaaa aacattgaac atcactgaaa aaacaggagc tttaatttct
      961 ataaatgcag taaccgatga agatgattta atgattatta ataaatctgg tttgactatt
     1021 agaatggaag tttctgattt aagagttatg ggaagagcaa cgcaaggtgt tcgtttaatt
     1081 aatataaaag gaaacgattc tattgccgca gtaaccaaag taatgaaaga agatgaaatt
     1141 gaagaaactg aattagaagg tacagaaata aatgaaaacg atgttgagtc aagtcaaaat
     1201 gaaacatcaa ctgaaaatac agaaaccgaa gattaactaa aattttagaa atatgaaaat
     1261 tagacaaatt atattagcat cagcattgct agtttcagtt tcaaatttcg ctcagaaaga
     1321 cgaattaaaa aaactgaaaa agatttatga taaagacgtt ccatctgcaa aagaagttga
     1381 ggaatacaaa atgaatgttg ctagtttgga aaaagtagct tctgacgaag ctgataaaat
     1441 ggcacttaat tattataaag ttaacattcc acaaatggaa cttgcatcta aagggcaagt
     1501 accaaacatg atggatttgc aaagagttta tacgccaaaa tatatttctg aattagcgaa
     1561 aacttatagt gatgttattg attttgaaaa aaaatcagga aaaaaaacat ttacagatga
     1621 tattaatgaa gaatttgctc aatctaagcc tattatatta aaccttgcca ttgaaatggg
     1681 aaatcaaaag aagttcaaag aaagcgcaag tatatttcat tctttgtatt taatggataa
     1741 aaaagacgtt gaccaacttt attatgctgc cagctatgct actaatggtc aagattatga
     1801 cacggcttta gaatattata atgaattaat tcgtcttaat tatatagggg atggcatgat
     1861 ttattatgca gttaataaaa caaacaagca agaagaatct tttggaaata ataaaagtac
     1921 acgagataca tttgttaaag taggtactta tgaaaaacct agagatgaga aaattgagtc
     1981 taaaaaagct gaaatttata aaaacgttgc tttgatatta gtacaacaag gtaaaacgga
     2041 cgaagcaatg aaggcagttt cacaagcaag aattgaaaat ccagatgatg caaatctaat
     2101 tttgacagaa gctgatttat atttaaaatt gaaagacatt actacttata aaaaattaat
     2161 aaatgaagcc ttagataaag atcctaataa tgccgattta gtatataatt taggagttat
     2221 aagttctcaa gcagatcagc tggaagacgc tatcaaatac tataaaaaag ctatttcaat
     2281 taaaccagat tattttaatg catatttaaa tttatcagaa gtcatgcttc gtggcgacga
     2341 aaaatatgtt gaagaaatga acaaactaac tacatcagat aaagataata agcgatatgc
     2401 ggtaattaaa gccgaacgcg agaaaaattt ccaagcagta ttaccttatt tggagaaagc
     2461 agtagaatta aaacctgaca atgaaccggc tgcaaaaaca ttaatgagtg tttataatgc
     2521 acttgaaatg acagataaat ataaagcttt aaaagctaaa atgaataatt aattcagaat
     2581 attaaataaa aaaaggctca caatttgtga gccttttttt atataaaatt tatcatttat
     2641 aaatgtatta cttcaccgta agcatcagca acagcttcca taacagcttc gctcattgtt
     2701 ggatgtggat gaatagcttt taagatttcg tgtcctgtgg tctctagttt tcttgctaca
     2761 actgcttctg caatcatatc agtaacacca gcaccaatca tgtgacaacc taaccattca
     2821 ccatatttag catcgaaaat tacttttaca aaaccgtctt ttgcaccaga agcactagct
     2881 ttgcctgaag cagaaaacgg aaatttacca atttttagtt cgtatccttt ttctttggct
     2941 tgtttttctg ttagtccaac agatgcaatt tcgggtgtgg catatgtaca acctggaaca
     3001 ttgccataat caattgggtc aacatgcatt ccagctattt tctctacaca attgattcct
     3061 tcggcagaag caacgtgagc taatgcttgt cctggcgtaa catctccaat ggcataatat
     3121 cctggtacat ttgtttggtt gtaggcattt accaaaattt tatctttatc agttgcaatt
     3181 ccaacttctt ctaaacctat attttcaata tttgttttaa ttccaacggc agaaagtaaa
     3241 atgtcagctt caataatttc ttcaccttta gctgttttta cataagcttt tactccattt
     3301 ccagaagtgt caattttttc aacagatgaa ctagtcataa tggtaatgcc agcttttttc
     3361 atcgaacgtt ccatttgttt ggatacgtct tcatcttcaa ctggaacaac atttggcata
     3421 aattctacaa tagttacttg tgttcccata gaattataaa aatgtgcaaa ttcaacacca
     3481 atagctccag aacccactac tatcatagat ttaggttgtt cgggtagagt cattgcttgt
     3541 ctgtatccaa ttactttttt gccatcttgt ggtaaatttg gcaattcacg agagcgtgcg
     3601 ccagtagcaa taatgatgtg atcggcagaa tattcagtta cttttccgtc agctgcggtt
     3661 acgtctattt tttttcctgg tttaattttg ccaaaaccat caataacatc aattttgttt
     3721 ttcttcatca agaactgaac tcctttgctc attccgtctg ctacatcgcg agaacgtttt
     3781 accacagcag aaaagtcttt atcgaattct tttacggtta atccataatc tgaagcatgt
     3841 tttagataat caaatacttg agctgatttt aaaagtgctt tggttggaat acaaccccaa
     3901 ttaaggcaaa ttccacctaa attttctttt tctattatgg caactttaaa ccctaattgt
     3961 gaggcgcgaa ttgcagttac atatccacct ggaccacttc ctaaaacaat aatatcgtat
     4021 ttcatactgt atgattttct aaattaattg agattgcgaa tttaaggatt tattttaaaa
     4081 aattagttaa ttccttttca ttcttaagaa gtttgttcat ttcagtaaca ccatttttat
     4141 ttagatgaat gtcgttgtaa aaataattca ttttatcttt aatataggaa ttgcttaggt
     4201 ctataaatgg tatgtttagt tttttacaga ttgttttagg aatttcaaat gcttttgtat
     4261 cataggttga tttgtaaact ggagtttgta tgcaaatgag tagaatgtta ttttctttgc
     4321 ataacagttt taattcttct aaagcatcag tgattttatg tgtttcattt tgtt
//
LOCUS       DAOOBH010000136         4342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_22757_length_4342_cov_5.814089, whole genome shotgun sequence.
ACCESSION   DAOOBH010000136 DAOOBH010000000
VERSION     DAOOBH010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22757_length_4342_cov_5.814089"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PK218_10570"
     CDS             complement(<1..1039)
                     /locus_tag="PK218_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPW98995.1"
                     /translation="MKNITLVLFAVISVNHSISAQDDKYQWLEDVDGLKALEFVNIQN
                     KATEAILTSQKDYQNIYNKSLEIYNSAQKIAYPDIRGRYVYNFWKDKDHVRGIWRRCL
                     LADYTANKLNWETVLDIDALSEKDGKKWVFKGASGLYPNYNKFLIQLSDGGGDAVYVK
                     EFDAITKQFITNGFSIDESKGSVSYLDENTLVVSTDFGEGTMTTSGYPNQVRLWKRGT
                     QLKDAQLIFEGESTDVSSSGYVVHDGATSYLFIGRGITFFTSQKMIWKDNKLIILDLP
                     EDCSPSGILKNQLLVSLKSDWIVDNITYKNGTLISLNFTDLVKGKKEIKVIVAPDAFS
                     SIADVSTTKNNL"
     gene            1367..1990
                     /locus_tag="PK218_10575"
     CDS             1367..1990
                     /locus_tag="PK218_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW98996.1"
                     /translation="MTKIITTIALFICTFFFAQNKYEEGMAKSLGLWGEGKPTEASAL
                     MERIASAEKNNWLPNYYVALINTTEGFNPANKDKVNALLEKAQNALDQAMLISPNNPE
                     IMVVQGLIYTVMIVQDPMTNGMKYSPKVMEVYTKAKMIAPENPRVAFSKADFEINGAR
                     WTGADVNKLCEEIERSITLFENFKSDVPFYPNWGLDRAKESLKNCKK"
     gene            2027..3361
                     /locus_tag="PK218_10580"
     CDS             2027..3361
                     /locus_tag="PK218_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HPW98997.1"
                     /translation="MNIKKDIALLFRITIIVFIAIQLLNVVLGNKIIFDEHLLVAFGF
                     TFMYSFSIGFANTYLNYKLDTIFVKDKFSRVRIVLNIFISFIITIAIIFLLRVFEEVL
                     VNKRTFEDYISHEKPSNFIVATVLTFIIILSIYLFHFYKAYKENQLKEQKVIAGTASA
                     QFESLKNQIDPHFLFNSLNVLSSLIEENPENAQKFTTSLSKIYRYVLEQKDKELVSVE
                     EELHFAKTYMNLLKMRFENSITYEIPTDFINPEAKVVPLSLQLLLENTIKHNVVSENK
                     PLKIKIYIEDNYLIVENNLQKKEVLQERKGVGLQNIVNRYGLLSERKVLVEETTDFFR
                     IKIPILTKQVVIMETQNIYNENMAYMKAKERVEKLKGFYSNLISYCCVIPLLIFINLR
                     TSSFQWFWFPMLGWGMGLSFHAFETFGFGKSWEEKKIQELMNKNTKSNQKWN"
     gene            3364..3690
                     /locus_tag="PK218_10585"
     CDS             3364..3690
                     /locus_tag="PK218_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HPW98998.1"
                     /translation="MRKRFKKSGNLELDLIKKRVHELKNFYVHLFIYCIGVLLYIGKR
                     YFDLPINFWPINFINDFFMWVWTFIIAVQGIKLFFKEMLFGTKWEQQKIKKLMEEEKS
                     ETTKWN"
     gene            3693..3998
                     /locus_tag="PK218_10590"
     CDS             3693..3998
                     /locus_tag="PK218_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HPW98999.1"
                     /translation="MEQHTNDYEKYKKAQKQVREIKGFYTHLFIYIIVMIGLVYINLR
                     FSPQYLWFIWSMSGWGIGVIFHGMKAFNYTPFLGKKWEEQKIKEFMEEEANNKKKYE"
     gene            4001..4309
                     /locus_tag="PK218_10595"
     CDS             4001..4309
                     /locus_tag="PK218_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HPW99000.1"
                     /translation="MENLEQLKYDQALKRVKRIKGFYTHLIVYIVINIAVFISNYQQL
                     EPNEPFFGIKLFSTPLFWGIGLLAHGLSVFMPTWFLGKDWEERKIKELMEKQKNNKWE
                     "
BASE COUNT         1553 a          655 c          683 g         1451 t
ORIGIN      
        1 gcaaattgtt ctttgttgta gaaacatctg caatactcga aaaagcatcg ggtgcaacaa
       61 ttactttaat ttcttttttt cctttaacta aatcggtaaa atttaaactt attaatgttc
      121 cgtttttata ggttatgtta tctactatcc aatctgattt taaagatact aacagttgat
      181 tctttaagat tccgcttggg ctacaatctt cgggcaaatc taatatgatt aatttattat
      241 ctttccaaat catcttttga gaagtaaaga aagtgatgcc acgaccaata aaaagataac
      301 tagtagcacc atcatgaaca acatatcccg atgaacttac atcagttgat tcgccttcaa
      361 aaattaattg tgcatcctta agctgtgttc ctcttttcca taatctcact tgattaggat
      421 agcccgaagt cgtcatcgtg ccttctccaa agtcggtaga aacaactagt gtgttctcgt
      481 ctaaataact cacacttcct tttgattcat ctattgaaaa accgtttgtt ataaattgtt
      541 ttgtgatggc atcaaactct tttacataaa cggcatcacc tccaccatcc gaaagttgaa
      601 ttaaaaactt attgtaattt gggtacaatc cggaagcacc tttgaacacc cattttttgc
      661 catctttttc agataaagca tcaatatcaa gaacagtttc ccagttaagt ttattggcag
      721 tataatcggc tagcaaacat ctacgccaaa tgcctcttac atggtcttta tctttccaaa
      781 aattataaac atatcttcct cttatatcag gataagctat tttttgtgca gaattgtata
      841 tttctaaact tttgttgtaa atgttttgat aatctttttg actcgttagt atagcttcgg
      901 ttgctttgtt ttgaatattt acaaattcta atgcttttaa tccatctacg tcctccaacc
      961 attggtattt gtcgtcttgt gccgagatgg aatgattaac actaattact gcaaatagca
     1021 ctaaagtaat attcttcatg atgcttttat atttttttta tgtgtttcaa atatagtgat
     1081 tttgtttatt cggatatggt attggtttat cataattttt gacacttgtt ccaattttat
     1141 ttaatttgta tttttcatct actttatcta tcaacttaaa ctgatttttt actcattatc
     1201 aaaaaattta attgatattc tataaattta caattcatcc taaaaaaaca acagttcggt
     1261 tagtaaatgt tttataagag tgtaaaccga agtagtttta caccatcaaa aagaaacaag
     1321 ttttaatcat caaacaaatc taataacatt taaaaaccaa aacatcatga caaaaattat
     1381 cacaaccatc gcattattta tttgtacttt cttttttgca caaaacaaat atgaagaagg
     1441 aatggcaaaa tcactcggtc tttggggcga aggaaaacca acagaagctt ctgctttaat
     1501 ggaacgaatt gcttcggctg aaaaaaacaa ttggcttcca aattattatg tagcattaat
     1561 caacacaacc gaaggtttta atcctgcaaa caaagacaag gtaaacgcat tattggaaaa
     1621 agctcaaaat gctttagacc aagccatgct aatcagtcct aacaatcctg agattatggt
     1681 tgttcaagga ttgatttata cggtaatgat tgtacaagac ccgatgacta atggaatgaa
     1741 atattcgcct aaagtgatgg aagtatatac taaagccaaa atgattgcac cagaaaaccc
     1801 aagagttgct ttctcaaaag ctgattttga gattaatggc gcaagatgga caggcgctga
     1861 tgtaaacaag ttgtgtgaag aaattgaacg ttcaataacc ttgtttgaaa actttaaatc
     1921 ggatgttccg ttttacccaa attggggatt ggatagagct aaagaaagcc taaaaaactg
     1981 taaaaagtaa attttagtac tctaaatata agtaaagaca ccacaaatga atattaaaaa
     2041 agacattgca cttcttttta gaataacaat tattgttttt atagcgatac aacttctgaa
     2101 tgtagttttg ggcaacaaaa taatttttga cgagcatttg ttggttgctt ttggctttac
     2161 ttttatgtat agttttagta ttggttttgc caatacatac ctaaattata agttggatac
     2221 gatttttgta aaagataagt tttcaagagt tcgcattgtt ttaaacatct ttatttcttt
     2281 tataataacg atagccatta tattcttatt gcgtgtattt gaagaggttt tggttaataa
     2341 aagaactttt gaggactata tatctcatga aaaaccgtca aactttattg ttgcaacagt
     2401 acttacattc attattattt taagtattta tctgtttcat ttttacaaag cgtataaaga
     2461 aaatcagtta aaagaacaaa aagtaattgc aggaacggca tcggctcagt ttgagagttt
     2521 aaaaaaccaa atagatccac attttctttt caatagtctg aatgtgttga gttcgcttat
     2581 agaagaaaac cccgagaacg ctcagaagtt taccacttcg ttgtctaaaa tatatcgtta
     2641 tgtgttagaa caaaaagaca aagaactggt ttcggtagaa gaagaattac attttgccaa
     2701 aacctacatg aacttattaa aaatgcgttt tgaaaacagt ataacatacg aaataccaac
     2761 cgatttcatt aatccagaag caaaagtagt tccgttatcg ttgcaattat tattagaaaa
     2821 tacaataaaa cacaacgtag ttagtgaaaa caaacctttg aaaattaaga tatacattga
     2881 agataattat ttaattgtag aaaataattt gcagaaaaaa gaagttcttc aagagcgaaa
     2941 aggtgttgga ttgcaaaata tagttaaccg ttatggctta ctatccgaac gaaaagtatt
     3001 agtagaagaa acaactgatt tcttccgaat taaaattcca atattaacca aacaagtcgt
     3061 cattatggaa acacaaaata tatacaacga aaacatggct tatatgaaag ccaaagaacg
     3121 cgtagaaaaa ctaaaaggat tttatagcaa tttgatttct tattgctgtg tcattccgct
     3181 cttaatcttc atcaacctaa gaacttcaag ctttcagtgg ttttggtttc caatgttggg
     3241 ttggggaatg ggactttctt ttcatgcttt tgaaactttt ggtttcggaa aatcttggga
     3301 agaaaaaaag atacaagagc ttatgaataa aaacacaaag tctaatcaaa aatggaatta
     3361 atcatgagaa aaagatttaa aaaatcagga aatcttgagc tagacctcat aaaaaagcgt
     3421 gttcatgagt taaagaattt ttatgttcat ttgttcattt attgcattgg agtactactt
     3481 tatattggaa aaagatattt tgatttacca atcaattttt ggccaatcaa ttttattaat
     3541 gattttttta tgtgggtttg gacttttatc attgcagtac aaggcataaa actttttttt
     3601 aaagaaatgc tttttggtac aaaatgggaa caacaaaaaa tcaagaaatt aatggaggaa
     3661 gaaaaatcag aaactactaa atggaactaa tcatggaaca gcatactaac gattacgaaa
     3721 aatataaaaa agcacaaaaa caagtgcgag aaatcaaagg gttttatacc cacttgttca
     3781 tctatatcat tgtaatgatt gggttagttt atatcaactt gcgtttttca ccacaatatt
     3841 tatggtttat ttggtcaatg tctggttggg gaattggagt catttttcac ggcatgaaag
     3901 cctttaatta tacccctttt ttaggtaaaa aatgggaaga gcaaaagatt aaagaattta
     3961 tggaagaaga agctaataat aaaaagaaat acgaatgatt atggaaaact tagaacaatt
     4021 aaaatacgac caagcactca aaagagtgaa acgcatcaaa ggtttttaca cccatttaat
     4081 tgtgtatata gtaattaaca ttgctgtttt tatttcaaat tatcaacaat tggaaccaaa
     4141 cgagcctttt tttggtatca aactttttag cacaccactt ttttggggaa ttggtttatt
     4201 agcacatggc ctatccgttt ttatgccaac ttggtttttg ggtaaagatt gggaagaaag
     4261 aaagataaaa gaactgatgg aaaagcaaaa gaataataaa tgggaatagt ataaatcaca
     4321 attatggaaa tacttttcaa aa
//
LOCUS       DAOOBH010000137         4329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_22838_length_4329_cov_7.534628, whole genome shotgun sequence.
ACCESSION   DAOOBH010000137 DAOOBH010000000
VERSION     DAOOBH010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22838_length_4329_cov_7.534628"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..860)
                     /gene="pyrH"
                     /locus_tag="PK218_10600"
     CDS             complement(153..860)
                     /gene="pyrH"
                     /locus_tag="PK218_10600"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPW99001.1"
                     /translation="MKYKRILLKLSGEALMGDRQYGIDPKRLAEYAEEIKEIHSQGIE
                     IAIVIGGGNIFRGVAGASNGMDRVQGDYMGMLATVINGMALQGALEDKGMLTRLQTAL
                     KIEAIAEPYIKRRAVRHLEKGRIVIFGAGTGNPYFTTDTAAVLRGIEVHADVILKGTR
                     VDGIYDADPEKNPNAVKFEEITFDDVIKKGLNVMDSTAFTLSQENALPIVVFDMNKSG
                     NLLKICNGENIGTTVRI"
     gene            1025..1621
                     /locus_tag="PK218_10605"
     CDS             1025..1621
                     /locus_tag="PK218_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPW99002.1"
                     /translation="MKTIIQNSLANSHTYSEYRTLVSSLLKEEKSTGNEQSEDLTHYS
                     QLNETRMNRLDKTIKVTEENNQKLQNLDKEYLWLVISEGWCGDAAQIVPVIHKMAELS
                     NKIELKIVLRDENEALMNLFLTNGSKSIPKLIIIDKATSEVIGDFGPRPTGAKQLILD
                     YKKEHGVIDETAKTELQLWYLHDKGVSTQNEIMEVLGV"
     gene            complement(1610..2302)
                     /gene="truB"
                     /locus_tag="PK218_10610"
     CDS             complement(1610..2302)
                     /gene="truB"
                     /locus_tag="PK218_10610"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPW99003.1"
                     /translation="MTGEDFQNGQILLIDKPLTWSSFQAVNKLKYLLKRKYNLPKSFK
                     IGHAGTLDPLATGLLIVCTGKFTKKITEIQGQAKEYTGTITVGATTPSYDLETEVDET
                     FPIVHISEELIQETTKQFLGEINQKPPIFSAIKKDGIRLYEHARAGETVEIPFRKTTI
                     HEFEITRVALPEIDFRVQCSKGTYIRSLAFDFGNALQSGAHLSALRRTKIGDYSVENG
                     FTPEDFEKQLNS"
     gene            complement(2303..3100)
                     /locus_tag="PK218_10615"
     CDS             complement(2303..3100)
                     /locus_tag="PK218_10615"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPW99004.1"
                     /translation="MDIIQAIILAVIEGITEFLPVSSTGHMIIASSFFGIAHDDFTKL
                     FTIVIQLGTILSVVVLYFKRFFQTLDFYFKLFVAFIPAVIFGLLFSDKIDALLENPIT
                     VAVSLVVGGIILLKVDEWFAPKEELQATENISYLTAFKIGMFQCLAMIPGVSRSGASI
                     VGGMSQKLSRTTAAEFSFFLAVPTMLGATVKKCYDYYKDGYVLTDDQVNLLIIGNVVG
                     FIVALLAIKSFIGYLTKHGFKIFGYYRIIAGLTILFIHFFILKLTTI"
     gene            complement(3100..3345)
                     /locus_tag="PK218_10620"
     CDS             complement(3100..3345)
                     /locus_tag="PK218_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HPW99005.1"
                     /translation="MENNSEHKPDFLFEKINYKILLIGVAVIMLGFILMSGGGSDNPN
                     VWSDAIFSFRRIRLAPTVVLIGFGITIYSIFKNPTKK"
     gene            complement(3386..4261)
                     /locus_tag="PK218_10625"
     CDS             complement(3386..4261)
                     /locus_tag="PK218_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPW99006.1"
                     /translation="MSSSFEKFQKRRVVTSYFSVVLSVFLVLFLLGILGLFVINSKRI
                     SDNFKEDIVMTVFLKSDVNDSIVKAFDDELRLASFAKDFKYVSKEEAAEKHKEVIGED
                     FMTFLGVNPLQNSYDIHLKADFVTNDSFGKIEKRLRKNEAIADIVYDKQLVTLVNDNV
                     KKISMWILIISGIFAFVSVLLINSSMRLSIYSNRFIIKTMQLVGATKSFIRKPFITRS
                     IILGMIGAVLAVAALVGVLAYIELNYPDLEILKDSAQTGIVLVAVLILGALIPWISTF
                     FATQRFLNLRTDDLY"
BASE COUNT         1486 a          756 c          641 g         1446 t
ORIGIN      
        1 tttctaaatg cgcaattgtt ccactcatgg attcttttgt gctgtctaaa ataaattcta
       61 tttcttccat ttttataaag tttaaacatt taaggattta aaagtttaag gattatgaaa
      121 ttataaactc ataaccatat atacctctaa tcttaaattc ttactgttgt tcctatattt
      181 tctccgttac agatttttaa taaattaccc gatttattca tgtcaaaaac cactattggt
      241 aaagcatttt cttgacttaa tgtaaatgct gttgaatcca taacattcaa tcctttttta
      301 ataacatcat caaaggtaat ttcttcaaat tttacagcat ttgggttttt ctcaggatca
      361 gcatcataaa ttccatcaac acgtgttcct tttaatatta catcagcatg aacttcgatt
      421 ccgcgtaaaa cagctgccgt atctgttgta aaatagggat ttcctgttcc tgctccaaaa
      481 atcacaattc ttcctttttc aagatgacgc acggctctac gcttaatgta aggttcagca
      541 attgcttcaa tctttaaagc cgtttgcaat cttgtcaaca ttcctttatc ttccaacgca
      601 ccttgcaaag ccattccgtt gattacagta gcaagcattc ccatataatc accttgaact
      661 ctgtccattc cattacttgc accggctact cctctaaaaa tatttccgcc accaattaca
      721 atagcaattt caataccttg actgtgaatt tccttgattt cttctgcata ttcggccaat
      781 cgttttgggt caattccata ttgtctatca cccattaaag cttcaccgct taattttagt
      841 agaattcttt tgtatttcat tttattctga atttatttca gaatctcata atcaagattc
      901 agatttgtga tgcaaatata aacatagttc tgtttttttt ataattcaat aaaagaatgt
      961 tttgtaataa atgtaacaaa ttataagttt tgtgtttcgt agatttgcat ttcaaattta
     1021 aattatgaaa acaattatac aaaatagctt ggcaaatagt catacttatt cggaatacag
     1081 aaccttagtt agttcactgc taaaagaaga aaaatcaaca ggaaatgagc agtcagaaga
     1141 tttaacacat tattctcagc tcaatgaaac tagaatgaat cggttggata aaactataaa
     1201 agtaacagaa gaaaataacc aaaaacttca aaacttggat aaagaatatc tttggttggt
     1261 tatttctgaa ggttggtgtg gcgatgcagc acaaattgtt ccagtaattc ataaaatggc
     1321 ggaattgtca aataaaatag aattaaaaat tgtgcttcgt gatgaaaatg aagccttaat
     1381 gaatttgttt ttaacgaacg gctccaagtc aattcctaaa ttaattatta ttgataaagc
     1441 tacaagcgaa gtgattggag attttggacc aagaccaacg ggtgcaaagc aattaatttt
     1501 agactataaa aaagaacacg gtgtcattga tgaaactgca aaaaccgaat tgcaactttg
     1561 gtatttgcac gacaaaggcg tttctacaca aaatgaaata atggaagttt taggagttta
     1621 attgcttctc aaaatcttct ggcgtaaatc cgttttcaac ggagtaatca ccaattttag
     1681 ttcttcgcaa tgcactaagg tgtgcgccag attgcaaagc atttccaaaa tcaaaagcca
     1741 aagaacgtat gtaagttcct ttgctgcatt gtactctaaa atcaatttca ggcaatgcaa
     1801 ctctagtgat ttcaaactcg tgaatcgttg tttttctaaa tggaatttca acggtttcgc
     1861 cagcacgtgc atgttcatac agtcggatac catctttttt aatggctgaa aagataggtg
     1921 gcttttggtt tatttctcct aaaaattgtt tggtagtttc ttgaattaat tcttcagaaa
     1981 tatgcacaat tggaaacgtt tcatcaactt cagtttctaa atcataactc ggtgtagtag
     2041 caccaacagt tatcgttccg gtatattctt tggcttgacc ttgaatttca gtaatttttt
     2101 tggtgaactt accagtacat acaattaata aaccggttgc caatggatct aaagttcctg
     2161 catgaccaat tttaaaactt ttgggcaaat tgtattttcg cttaagaaga tatttcaatt
     2221 tattcactgc ttgaaatgaa gaccaagtta atggtttgtc aattaaaaga atttgaccgt
     2281 tttgaaaatc ttcacctgtc atctatattg tggtcaattt tagtataaag aaatgaataa
     2341 acaatatggt taaacctgca atgattctat agtaaccaaa gattttaaaa ccgtgttttg
     2401 ttaaatatcc aataaacgat ttgatggcta ataaagcaac gataaaacca acaacattgc
     2461 caataatcaa taaatttact tgatcgtctg tcaatacata tccgtcttta tagtagtcat
     2521 aacatttttt taccgttgcg ccaagcatgg taggaacagc tagaaaaaaa gaaaattcgg
     2581 cagctgtagt tcttgataat ttttgtgaca ttccaccaac aatacttgcg ccacttcgtg
     2641 aaacgccggg tatcattgct aagcattgaa acattcctat tttaaaagct gttaaataac
     2701 taatgttttc ggttgcttgt aattcttctt ttggtgcaaa ccattcgtca actttcaaca
     2761 gaataattcc gccgacaact aaagaaacag caacggttat tgggttttct aataaagcat
     2821 caattttatc actaaacaat aatccaaaaa ttaccgccgg aataaacgca acaaatagtt
     2881 taaagtaaaa atctaaggtt tgaaagaagc gtttgaaata taaaacaact actgataaaa
     2941 tagttcctaa ttgaattaca atcgtaaata atttggtaaa atcatcatgt gcaatgccaa
     3001 aaaatgaaga ggcaataatc atatgaccag tagaagaaac aggaagaaac tctgtaatac
     3061 cttcgataac cgcaaggata atggcttgaa taatatccat tattttttgg ttggattttt
     3121 aaatatagaa taaattgtaa ttccaaaacc gattaaaaca acagtcggtg ccaatcgaat
     3181 tcgtctaaaa ctaaaaatag catcactcca aacatttgga ttatcgcttc caccgcctga
     3241 cattagaatg aatcctagca taattacggc aacaccgatt aataaaattt tataatttat
     3301 tttttcaaaa agaaaatcgg gtttgtgttc gctattattt tccatgtcta ttttttttaa
     3361 aatttatttt tccaacttca aaattctaat aaaggtcgtc tgttcttaaa tttaagaaac
     3421 gttgtgtggc aaagaaggtg ctaatccaag gaattaatgc cccaagtatt aatacagcaa
     3481 ccaatacaat tccagtttgt gcactgtctt ttagaatttc taaatcagga taatttaatt
     3541 cgatataagc caaaacgccg accaaagctg ccacagctaa gacggcacca atcattccta
     3601 aaatgatgct tcgggtaata aaaggttttc tgatgaatga ttttgtagca ccaaccaatt
     3661 gcatggtttt gatgataaat cgattggaat atatcgacaa gcgcatcgaa ctattgatta
     3721 ataaaacgga tacaaaagca aatattccac tgattattaa aatccacata ctaatttttt
     3781 tcacgttatc gtttaccaaa gtaaccaatt gtttgtcgta aacaatgtcg gctattgctt
     3841 cgtttttgcg caatctcttt tcaattttgc caaagctatc attggtcaca aaatctgctt
     3901 ttaagtgaat atcgtatgag ttttgaagcg gattaacccc taaaaatgtc ataaagtctt
     3961 cgccaattac ttctttatgt ttctcagctg cttcttcttt tgaaacatat ttaaaatctt
     4021 ttgcaaaact ggctaaacgc aattcatcat caaacgcttt tacgatagaa tcattgacat
     4081 ccgattttaa aaaaaccgtc attacaatat cttctttaaa attatctgag atacgtttag
     4141 agttgattac aaataatccc aagataccta aaaggaaaag cactaaaaat acacttagta
     4201 ccaccgaaaa ataggaggta accactctgc gtttctgaaa cttttcgaaa gatgacgaca
     4261 tatataataa ttttatggtg taaaaataat aaagaaaatt gtttttatcc tttataaggt
     4321 acaaagttt
//
LOCUS       DAOOBH010000138         4265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_23275_length_4265_cov_4.556057, whole genome shotgun sequence.
ACCESSION   DAOOBH010000138 DAOOBH010000000
VERSION     DAOOBH010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23275_length_4265_cov_4.556057"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="PK218_10630"
     CDS             <1..1455
                     /locus_tag="PK218_10630"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPW99007.1"
                     /translation="ILDTYNTLWFGSKNGELIYYNGKNATDYDEKNFISIKHPLFENG
                     KTITFIHQWHDYLILGASNKILLFDLKKWYANKTVSVRYLNSMEINLTSETEQNTILT
                     DKRDNSIWFATSDMVYQWDIKKWLSLPTFKVVPTIVVKKDSTQIEFSRNRTINFKPTE
                     NSFDIQINYQTKDNMPRFINCVLVKKGDKLMFGQPNLQTKYQYMNLSSGDYIFYIRVC
                     QQDGSFEVFEYPICIDSFLWQKWWFWLLILLSIVGVLFYFFLKRNEIEKQKKKLSQLN
                     LSSLSNQFRPHFMLNALNSIGSQMQGKPHAEKVISRLGESINILYGFTQKNEFTLPFK
                     NEWKLVENSIEIQKLLFIPDLQFTVTNENLVPVDYKIPVGLLQIPVENALLHGLRNKI
                     DGNCVLEIDFKEDETHYFVTITDNGVGREEAIKINNFKKNGNGLKTIFEMITIINQHQ
                     KNAIVFEIIDQEAPTGTIVKITLHKNIDYEKIKI"
     gene            1436..2170
                     /locus_tag="PK218_10635"
     CDS             1436..2170
                     /locus_tag="PK218_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99008.1"
                     /translation="MKKSRYSYVVIEDDLNVCEGVKERMDAFTNWDCLGLIPVFDVAL
                     QAIQKDKPNLLFLDYSILGGNTFNLLEQIKSIDNYNPFIIYFTAYGSDNTFISEDAIN
                     KYKVNKFLNKPIWEKLTDYLVDFINEAEAWVLKNENSELWLITSDKIKVKIEPYKIIC
                     INQPENNPRHKTIRTIDHKIFDIKASWEECETIAKDFSINYCYSKSRDTIINKKYITK
                     IQKPYIWLNDNLKITVTKERWKDIDV"
     gene            2339..2821
                     /locus_tag="PK218_10640"
     CDS             2339..2821
                     /locus_tag="PK218_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99009.1"
                     /translation="MEASFETLIASYIQNNVGIATDFLSVALCEHLAANILTLEANEL
                     LQQAGIGNDNKLQQNALVRRDTIYWLDKKHNNIHEDAFFAKIDAFVLYLNQSCYAGIT
                     DYEFHYSLYETDDFYKKHLSIQDLFYVFYFKLIISSQFYNPIININFISIPDDITIIR
                     "
     gene            complement(2738..4081)
                     /locus_tag="PK218_10645"
     CDS             complement(2738..4081)
                     /locus_tag="PK218_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HPW99010.1"
                     /translation="MRTTNFMSLLFLLTIGLTSSCGSDGGSGDGNNPPGNPPSSIEWQ
                     KAIGGTQSDYGQTVTQTNDGGYIVAGYSQSTNGDVEGHHGPYYFNDIWVVKFNGDGLV
                     QWKKSIGGSGEDRAYAIKQTTDGGYIIVGTANSSDGDVLGGQVTGASGWVVKLTSSGN
                     IDWQKTYDYDGGSNEILYAVVQTSDGGYTVAGYSEHGNGIGASFWILKLNATGVTVWE
                     KFYGGGNDDRAFSLNKCLDGGYIVVGRTSSNDGNVTGNHGDFDWWVIKLSSGGDLEWQ
                     KAFGGSGMDEAYAVDQTSDGGYIIGGWTGSADGNVTDYNGNGDFWIVKLNSSGSMVWQ
                     KAYGGTSVEKAYAIQQTSDDKYIVAGYASSSDGFVTGNHGSDDFWLVKLDGSGDLIWQ
                     KTLGGSGLDQAKSIEQTNNGGYIIAGVTSSDDGDVIGNTDEVNVDYWVVKLTGNN"
BASE COUNT         1498 a          778 c          600 g         1389 t
ORIGIN      
        1 attcttgata cttataacac cttatggttt ggctcaaaaa atggagaatt aatatattac
       61 aatggtaaaa atgctactga ttatgatgag aaaaacttca tttcaataaa acatccttta
      121 tttgaaaacg gaaaaacaat tacgtttatt catcaatggc atgattattt aatacttggt
      181 gcaagtaata aaatcttact ttttgatttg aaaaaatggt atgctaacaa aactgtttca
      241 gttcgttatt tgaattcaat ggaaattaat ttaaccagtg aaactgagca aaatactatt
      301 ttaacagaca aaagagataa tagtatttgg tttgcaactt ctgatatggt ttatcaatgg
      361 gatattaaaa aatggttatc gctgccaact tttaaagtag ttcccactat tgttgtaaaa
      421 aaagacagta ctcaaattga attctcaaga aatagaacta tcaattttaa acccacggaa
      481 aatagttttg atattcaaat aaattatcaa actaaagata atatgcctcg atttattaat
      541 tgtgtattag taaaaaaagg agataagcta atgtttggtc aacctaattt gcaaacaaaa
      601 tatcaatata tgaatttatc ttcaggtgat tatatttttt atatcagagt ttgccaacaa
      661 gatggtagtt ttgaggtgtt tgaatatcct atttgtattg atagtttttt atggcaaaaa
      721 tggtggtttt ggttgttaat tttactttca atagttggtg ttttgtttta ttttttttta
      781 aaaagaaatg aaattgaaaa acaaaaaaag aagttgtcgc aattaaatct ttctagcttg
      841 agcaatcagt tccgacctca ttttatgcta aacgccttga atagtattgg ttcgcaaatg
      901 caaggcaaac cacatgcaga aaaagtgatt agtcgcttgg gtgaaagcat taatatatta
      961 tatggattta cacaaaaaaa tgaattcaca cttccattta aaaacgaatg gaaattggtt
     1021 gaaaacagta tagaaataca aaagttactg tttattcctg atttgcaatt tactgtaact
     1081 aatgaaaatt tagtcccagt tgattataaa attccagttg gcttactaca aatccccgtt
     1141 gaaaatgctt tgcttcatgg tttgcgaaat aagattgatg gaaattgtgt tctagaaatt
     1201 gattttaaag aagatgaaac ccattacttt gttacgatta ccgataatgg ggtaggacgt
     1261 gaagaagcta ttaagattaa taatttcaag aaaaatggta acggtttaaa aactattttt
     1321 gaaatgatta cgattattaa ccaacatcaa aaaaacgcta ttgtttttga aattatcgat
     1381 caagaagcac ccactggaac aatagtaaaa attacattgc ataaaaatat agattatgaa
     1441 aaaatcaaga tatagttatg ttgttattga agatgattta aatgtttgtg aaggtgtcaa
     1501 agagagaatg gatgccttta cgaattggga ttgtttgggg ttaattcctg tcttcgatgt
     1561 ggcattacaa gcaatccaaa aagacaagcc taacttgtta tttcttgatt attccatact
     1621 gggtgggaac acttttaact tattggaaca aattaaatcc attgataatt acaacccatt
     1681 cattatttat tttacagctt atggtagtga taatacattt atttcagaag atgctattaa
     1741 taaatataag gtcaataagt tcttaaacaa acccatttgg gaaaaactaa ccgactattt
     1801 agttgatttt attaatgaag ccgaagcatg ggttttaaaa aatgaaaaca gtgagttatg
     1861 gttaattact agtgataaaa taaaagtaaa aattgaacca tacaaaatta tctgtatcaa
     1921 ccaacctgaa aacaatccaa gacataaaac cattagaact attgatcata aaatatttga
     1981 tattaaagca agctgggaag aatgtgaaac tattgctaaa gatttttcca taaattattg
     2041 ttattctaaa tctcgcgata ctattataaa caaaaaatac attaccaaaa ttcagaaacc
     2101 ttacatttgg ttaaatgata atttaaaaat taccgttact aaagagcgat ggaaggatat
     2161 tgatgtttaa caaaaattga atgctatttg ttattgtagt tccgtttgtc caactacgtt
     2221 tagaaattct caaaaaggta aaattcataa atcattcttt tttacaaata aaaaccactt
     2281 cctaacgttt attcatctac cttcacccaa tacttgtcat atcaggtaat gaagtaggat
     2341 ggaagccagt ttcgaaacct tgattgccag ttatattcaa aacaatgtag gcatcgctac
     2401 cgattttttg agtgttgctt tgtgcgagca tttagcagcc aacatcctta cactcgaagc
     2461 caacgaactg ctccaacaag ctggtattgg caacgacaac aagcttcaac aaaacgcctt
     2521 ggtgcgccgt gatactattt attggctcga taaaaaacac aacaacattc atgaagatgc
     2581 tttttttgca aagattgatg cctttgtgct atatctaaac caaagctgct atgcgggcat
     2641 caccgactac gaattccatt actcgctcta cgaaacagac gatttctaca aaaaacacct
     2701 ttccatacaa gatctttttt atgttttcta ttttaaacta attatttcca gtcaatttta
     2761 caacccaata atcaacatta acttcatcag tattcccgat gacatcacca tcatcagatg
     2821 atgtaacacc agcaataata taaccgccat tgtttgtttg ctcaattgat ttggcttggt
     2881 ctaatccaga accacctagc gttttttgcc aaattaaatc tccagaacca tctaatttaa
     2941 caagccaaaa atcatcactt ccatgatttc ctgtaacaaa tccatcacta gaagaagcat
     3001 acccagccac gatatattta tcatcagagg tttgttgaat agcataggct ttttccacgc
     3061 ttgttccacc ataggctttc tgccaaacca tgctcccaga actatttaac ttaacaatcc
     3121 aaaaatctcc atttccatta taatccgtca catttccatc agccgagcca gtccatccac
     3181 caatgatata gccaccgtct gaagtttgat caacagcata agcctcatcc attccagaac
     3241 ctccaaatgc tttttgccat tccaaatcac caccactgct taattttatt acccaccaat
     3301 caaagtcacc atgattccca gtaacatttc cgtcatttga acttgttctg cctactacaa
     3361 tatatccacc atctaaacat ttattaagtg aaaaagctct atcatcattt cctccaccat
     3421 aaaatttctc ccatacggtt actccagttg catttaattt aagtatccaa aaactagcac
     3481 ctattccgtt cccatgttct gaatatcctg caacggtata tccaccatca gatgtttgaa
     3541 ctacagcata taatatttca ttactaccgc catcataatc atatgttttt tgccaatcaa
     3601 tgtttccgga actagtaagt tttacaaccc atccacttgc tccagtaact tgaccaccca
     3661 aaacatcacc atctgacgag ttagccgttc caacaattat atacccacca tcagtggttt
     3721 gctttatagc gtaagctcta tcttctccag aacctccaat tgactttttc cactgaacta
     3781 aaccatctcc attaaatttc actacccata tatcattaaa ataatatggc ccatgatgcc
     3841 cttcaacatc accattagta gattgtgaat accctgcaac gatgtaacca ccatcattcg
     3901 tttgggttac agtttgtcca tagtcacttt gggttccacc tatagctttt tgccattcaa
     3961 tagatgaagg tggattacca ggaggattat taccatcacc actcccgcca tcacttccgc
     4021 aactacttgt taaaccaata gtaagtagaa ataataaaga cataaaatta gttgttctca
     4081 tacgttctct tttattttaa gttttagttc gtttcacaaa ggtattattt ttcaatatta
     4141 gaaaaaacaa aatattttga atgaaatgaa aaattaacac aaacaatgag gttgtcaaaa
     4201 agttttagta ttttacaaat aaaaaccact tcctaacgtt tattcatcta ccttcaccca
     4261 atact
//
LOCUS       DAOOBH010000139         4264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_23284_length_4264_cov_4.150867, whole genome shotgun sequence.
ACCESSION   DAOOBH010000139 DAOOBH010000000
VERSION     DAOOBH010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23284_length_4264_cov_4.150867"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PK218_10650"
     CDS             complement(<1..1547)
                     /locus_tag="PK218_10650"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99011.1"
                     /translation="MKMNTFLYKLLFIFITMTSFQGFSQCFEIQTILVDACSATSPSN
                     DEGFNEMVRFKIGPTALNTATLSVNWPSNAWTGLIQNATTASKVSTINNAIVAAGNCG
                     QVLEPTGGVLPANSEVLLITSQNFTVNFNSFSSLNSTLYIIFQNNTTTTGGHFGNYNA
                     TPGTRTLSMTFGGGCSDTVTYERSNLINSLGGSGGTSAEQDGAAVNFTPAGVPSYINN
                     GCTAPVTPFTVSVTANPNNCVAPGTVINLTGVAEGYQSLAWTTSNGTVTNPTNLNATY
                     TVPTNAVGTITFTLTATNACNATTSATVSISIGPAKPTVISPITYCQNAVATPLMATP
                     STGGTLNWYGTNATGGTASATAPTPSTAALGSTTYYVSQTVGGCESTRAAIVVIVTNQ
                     PPTATPSLFCDTANSGPNSVAFDFNNAGQTNFTYSYTIDGGLPVTGIHTSPTNFTVNG
                     VLPGQVVTFTLTWNGVCTPTQTASTTVPSFNQVAPICSGQTFTLPTTSINGKIGTWSP
                     AINNTATT"
     gene            complement(1679..2851)
                     /locus_tag="PK218_10655"
     CDS             complement(1679..2851)
                     /locus_tag="PK218_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPW99012.1"
                     /translation="MEKIFNNTETAFALKTDTELDRAYFLFKMIANEPLVRIGTAVTN
                     FAIKAHLPVEGLIRATVFDHFCGGVNEEDCLSVVDKMYTKGVSSVLDYSVEGKEEESQ
                     FDIALQMTLKTIEFAKERQAIPFAVFKPTGIGRFDLYVKIGEKKTLTDSEQIEWNRVV
                     ERFDIICKTAHEKDVALLIDAEESWMQDAADAIVTDMMRKYNKQKIIVFNTLQMYRWD
                     RLDYLKNLHQLAKSEGFLIGMKLVRGAYMEKENKRAEEKGYKSPICISKQATDDNYDA
                     SVLYMMEHLEIMSIFAGTHNEESSYQLMKMMQQKSITPSDSRIWFGQLYGMSDNISYN
                     LAEKGYNVAKYLPFGPVRDVMPYLIRRAEENTSVAGQTNRELNLLKTEKERRKANR"
     gene            2937..4004
                     /gene="aroB"
                     /locus_tag="PK218_10660"
     CDS             2937..4004
                     /gene="aroB"
                     /locus_tag="PK218_10660"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPW99013.1"
                     /translation="MQSINANEYSIHFSQNAYQFLSEIIENENYSNLFILVDSNTNEF
                     CLPHFLPNLATTKTIEIIEIEAGEEQKNINTCVEIWKILTELGGDRKSLLINLGGGVV
                     TDIGGFVASTFKRGIDFINIPTSLLAMVDASIGGKNGVDLGTLKNQIGVINVPKMVII
                     DTEYLADLPQNQMRSGLAEMLKHGLIYDKTYWEKFLDLSQSDGSDIDTLIHRSVAIKN
                     EIVSQDLTENGIRKSLNFGHTLGHAIESYFLENDAKNTLLHGEAIAVGMIMESYISFE
                     KKLISKTELELIKSILLHYFEKVIFIENDIEPIVNLLIHDKKNEYGKVQFCLLDGIGK
                     TIYNQEADNELINRAFNYYNS"
     gene            4061..4240
                     /locus_tag="PK218_10665"
     CDS             4061..4240
                     /locus_tag="PK218_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99014.1"
                     /translation="MKKHKNITIFNVSSQENIFLQTDLHLVYSSLIKNLTALVNPFEL
                     LFKEKLPIIYANIRV"
BASE COUNT         1361 a          739 c          700 g         1464 t
ORIGIN      
        1 gtagtagctg tattattaat agctggcgac catgttccaa tcttgccatt tattgatgta
       61 gttggtaatg taaaagtttg tcctgaacat attggagcta cttggttaaa gctaggaaca
      121 gtagttgagg cggtttgtgt cggagtacaa acaccattcc atgttaatgt aaaagtaaca
      181 acttgtcctg gtaaaactcc attgacggta aaattagttg gtgaggtatg aattccagtt
      241 acaggcagtc caccatcaat tgtataactg taggtaaaat tagtttgacc agcattattg
      301 aaatcaaaag ctacagagtt tggtcctgaa tttgccgtgt cacaaaacaa actaggtgtt
      361 gctgtgggag gttgatttgt aacaataaca actatagcag ccctagtgct ttcacatcca
      421 ccaaccgttt gacttacata ataggtagtt gatcctaatg ccgcggtaga tggagttggt
      481 gctgttgcac tagcagttcc tccagttgca tttgtaccat accaatttaa agttcctcca
      541 gtacttggtg tagccattaa tggagttgca actgcatttt gacaataggt tatcggcgaa
      601 ataactgttg gtttagcagg cccaatagat atagaaacgg ttgcgctagt ggtagcattg
      661 catgcatttg ttgcagtaag cgtaaaagtt attgttccta ctgcatttgt tggaaccgtg
      721 taggttgcat ttagattagt tggattcgta acagttccat tagaagttgt ccatgctaat
      781 gattgatatc cttctgccac tcctgttaag tttatgaccg tacctggtgc tacacaattg
      841 tttgggtttg cagttacact aactgtaaaa ggtgttacag gagcagtaca accattattt
      901 atataggaag gaactcctgc aggtgtaaaa tttactgcag caccatcttg ttccgctgat
      961 gttccacctg aacctcctag actatttatt aagttgcttc gttcataagt cacggtatct
     1021 gaacaacctc cgccaaaagt cattgacaaa gttctagttc ctggagttgc attgtaattt
     1081 ccaaaatgac cgcctgttgt cgtcgtatta ttttgaaaaa taatatataa tgtactattt
     1141 aaggatgaga atgaattaaa attaactgta aaattttgac tggttataag taatacttct
     1201 gaattggctg gcaaaacacc tccagttggt tctagtactt gaccacaatt accagcggca
     1261 acaatagcat tatttatcgt tgaaactttg gaagctgttg tagcattttg aattaaccct
     1321 gtccatgcat ttgaaggcca gtttacggat aaagtagccg tgtttaaagc cgttggtcca
     1381 attttaaacc gaaccatttc gttaaaccct tcatcatttg aaggagacgt tgcagaacaa
     1441 gcatcgacta aaattgtttg aatttcaaaa cattgtgaaa atccttgaaa ggaagtcatt
     1501 gtaataaaaa taaacagaag tttatataga aaagtattca ttttcatttt ttaaaattgc
     1561 ttttatttcc tcttttttat tttacaaaaa ggagccaaat atattgaatt caaatgcaac
     1621 atataattat tttgcagtaa ataattatat gaaatcttga aattgaacaa gtttaaagct
     1681 atctattcgc ttttcttctt tctttttctg ttttcaataa attcaattct ctattcgttt
     1741 gtcctgctac tgatgtattt tcttctgctc tgcgaattaa atatggcatt acatccctaa
     1801 cgggtccaaa tggtaaatac ttggctacgt tatacccttt ttcggctaaa ttataactga
     1861 tgttatcgct cataccatat aattgtccaa accaaattcg agaatcactt ggcgtgatac
     1921 ttttttgctg catcattttc atcaattgat acgaactttc ttcattatgt gttcctgcaa
     1981 aaatggacat tatctctaaa tgctccatca tataaagaac agatgcatca taattgtcat
     2041 ccgtggcttg ttttgaaatg catattggtg atttatatcc tttttcttcg gcacgtttat
     2101 tttctttttc catatatgcg ccacgaacca acttcatgcc aattaaaaac ccttctgact
     2161 tggctaactg atgcagattt ttcaagtaat caagtctgtc ccaacggtac atttgcaagg
     2221 tattgaacac aataattttt tgtttattgt attttcgcat catatcagta acaatggcat
     2281 ctgcagcatc ttgcatccaa ctttcttcgg catcaatcaa tagagcaacg tctttttcat
     2341 gtgctgtttt acagataata tcaaatcgtt ccactaccct attccattca atttgttcag
     2401 aatcggttag cgtttttttc tctcctattt taacatataa atcaaatcgt ccaattcctg
     2461 ttggtttaaa aacagcaaac ggaatagctt gacgttcttt tgcaaattca atagtcttta
     2521 aagtcatttg caaagcaata tcaaattgtg actcttcttc ttttccttct accgaataat
     2581 ccaacacaga tgaaacacct ttagtgtaca ttttgtcaac aactgaaaga caatcttctt
     2641 cattcactcc gccacaaaaa tgatcaaaaa ccgtggctct tatcaatcct tcaactggca
     2701 aatgtgcttt gattgcaaaa ttagtaaccg ctgtaccaat tcgaactaat ggttcgttag
     2761 ctatcatttt aaataaaaaa tatgctctat ctaattctgt atcagttttt agagcgaaag
     2821 cggtttcggt attgttgaat attttttcca tatcttaaaa taattacgca aatataatca
     2881 gactttataa ttattaagca aaatttacct tattttgcct tttaaattaa tcaagaatgc
     2941 aatctataaa tgctaacgaa tattccatcc atttttcaca aaatgcctat caatttttaa
     3001 gtgaaataat tgaaaacgaa aattattcca atctatttat attggtcgat agcaatacta
     3061 atgaattttg tttacctcat ttcttgccca atctggctac aacaaagaca attgaaatca
     3121 ttgaaattga agctggtgaa gaacaaaaaa acattaatac ttgtgttgaa atatggaaga
     3181 tactaaccga acttggtggc gatagaaaaa gcttactaat caatcttggt ggtggcgtag
     3241 ttacagatat tggaggtttt gtagcttcta cttttaaaag aggcattgat ttcattaata
     3301 ttccaacaag tttattggca atggttgacg catcgattgg cggcaaaaac ggtgtcgatt
     3361 tgggaactct aaaaaatcaa attggcgtta tcaatgttcc aaaaatggta attatagata
     3421 ccgaatattt agccgattta cctcaaaacc aaatgcgctc tggacttgca gaaatgttga
     3481 aacacggctt gatttatgac aaaacgtatt gggaaaaatt ccttgattta tctcaatctg
     3541 atggttcgga tatcgacacg cttatccatc gttctgttgc cattaaaaat gaaattgttt
     3601 ctcaagattt aaccgaaaat ggtattcgaa aatcattaaa tttcggtcat actcttggac
     3661 atgctattga aagttatttt ctagaaaacg acgctaagaa caccttgcta catggagaag
     3721 caatcgctgt tggaatgatt atggaaagtt atatttcttt tgaaaaaaaa ttgatctcta
     3781 aaaccgaact ggaacttatt aaatcaatac ttttacatta ttttgaaaaa gtaattttta
     3841 ttgaaaatga catagaacca atcgtgaatt tactcattca tgacaaaaaa aatgaatatg
     3901 gaaaagtgca attttgtttg cttgatggaa ttggaaaaac aatttacaat caagaagcag
     3961 acaatgaact gataaacaga gcattcaatt attacaactc ttaacttttt tttaatcatt
     4021 ttattggatt taggaaatat tattttttaa atttgcccta atgaaaaaac acaaaaacat
     4081 taccattttt aatgtttcaa gtcaagaaaa tatttttctt caaactgatt tgcacttagt
     4141 atattcaagt ttaataaaga atttgactgc cttggtaaat ccatttgaat tacttttcaa
     4201 agagaagcta cccattattt atgccaacat tagggtttga attgcaatag attttagtct
     4261 caaa
//
LOCUS       DAOOBH010000140         4180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_23866_length_4180_cov_6.350061, whole genome shotgun sequence.
ACCESSION   DAOOBH010000140 DAOOBH010000000
VERSION     DAOOBH010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23866_length_4180_cov_6.350061"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(634..1866)
                     /locus_tag="PK218_10670"
     CDS             complement(634..1866)
                     /locus_tag="PK218_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW99015.1"
                     /translation="MNLEYFTAKKLVSAKDHKSSISAPIIKIAITAIALGMIMMIISV
                     ATGIGLQQKIRQKVAAFNGHVIISSYNDNQSDVSTEPISIKQSFYPEFKNIDGINHVQ
                     AVASKAGMIRTENAFEGIIFKGIGTDYKSDNLSEYITEGRLPVLNKNLNNEVMISQYL
                     ANRLGLKLGDKFVTYFMKETSEGYNLRNFKIVGIYNSGFQEFDATYILGDIRHVQRIN
                     KWKPNQIGSFEVYIKDFSEIEYKGKEIYEETSSTLDSKTIVEKYYYIFEWLKLFDFNI
                     LIILIVMIAVSTINMVVALLVLILERTQMIGILKSLGANNWSIRKIFIYNATYLIGRG
                     LFYGNLIGIGLLLIQKYFGVIKLNPESYYVNEAPVDINLGYILLLNIGTVLVCLLVLL
                     IPSYLITKISPSKSIKFE"
     gene            1873..3090
                     /locus_tag="PK218_10675"
     CDS             1873..3090
                     /locus_tag="PK218_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPW99016.1"
                     /translation="MISKLVFKNTVLLFVLLILSCGSSIKKIENKERKEVVSFEKKFQ
                     EIKTGADNFTYYLPLLKGKKVGIVTNQTGILSNKTHLVDFLLEKNINLQKIFAPEHGF
                     RGTADAGEHVIDGKDSKTGLPIVSLYGDNKKPKPEQLKGIDVMLFDLQDVGVRFYTYI
                     SSLHYIMEACAENNVELIVLDRPNPNGSIVDGPILEKENSSFVGMHVIPLLHGMTIGE
                     YAQMIDGEKWLKNGIQCSLKVIPCLNYKREMSYSLPVKPSPNLPNDQSINLYASLCLF
                     EGTTISVGRGTEMQFQIYGSPYLPKSSFSFTPKPNFGAKDPVYNGKLCYGENLTTIPK
                     VSNLELKWLMKAYNETPDKTKFFNPFFTKLAGTKKLQQQIEAGIPEFEIRKSWEKGLN
                     DFKTMRKKYLIYD"
     gene            complement(3087..3488)
                     /locus_tag="PK218_10680"
     CDS             complement(3087..3488)
                     /locus_tag="PK218_10680"
                     /inference="COORDINATES: protein motif:HMM:NF036413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPW99017.1"
                     /translation="MKIYNSKIDWWLIIPFLIPIYFGIVEVLNHNKSGWIVIVVTVAF
                     VVFMYRSTYYCIENNTLTVRSMFIVHKKIEISSIRKIYKTRNPLSSPALSLDRIAIVY
                     NKHDEIMISPKDKIQFIEEMLRLNPGIEVKN"
     gene            complement(3490..3984)
                     /locus_tag="PK218_10685"
     CDS             complement(3490..3984)
                     /locus_tag="PK218_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPW99018.1"
                     /translation="MKPNLDELKKLAKDKHIENKKYFDKLKKRTPKNLDYVMQDLHDA
                     EFKKSNCLECANCCKTTGPLFTTADIERISKHFKQKPQQFIDQYLRIDEDKDYVLQNV
                     PCSFLDNENYCMIYDVRPKACREFPHTDRKKFQQISDLTLKNVSICPAAYNIVEEMKK
                     KMPF"
BASE COUNT         1415 a          644 c          580 g         1541 t
ORIGIN      
        1 tgcatgtgtt aggcctgccg ctagcgttca tcctgagcca ggatcaaact cttcatcgta
       61 tgttgttgat tctaacttta cgttagtctc gtttttttag acaaaggcta gtgtaattct
      121 ttacaattta cttactctct tattttgtct taatgtctcc attaaaacgg ctgtcaattc
      181 aatatgtcta tgaacgtttt cttttatatt ttcgccattc tttcggttag cgggtgcaaa
      241 agtacaactc ttttttaatc ctgcaagaaa aaattaaaat atttttttat tttttttctc
      301 ctcattaaat ctctaaaaaa ttaaccgttt tgcggacggc aaaggtaaaa ccttttttaa
      361 tcctaaacaa aacattttaa ttgttttttt taatttattt ctcagtgtat ttatagaacg
      421 tctgccttaa tgcgggtgca aaagtaagct tcttttccaa tctcacaaac ttattttaac
      481 ccttttttta aagtattttt taatgccctc attgtgcgta ctttattctt gaatttattt
      541 tgaataagca gaaaaacata gtagctttgt tgttactttg attttaaaat atcactaaat
      601 atactgtttt gctaatgttt caaaaataaa aatttattcg aatttgatag attttgaagg
      661 agatattttt gtaattagat aggaagggat taacaaaact aataaacaaa caagcactgt
      721 acctatatta agtaataata tgtagcctaa attaatatca actggtgctt catttacata
      781 atagctttcg ggatttaatt taattactcc aaaatacttc tgaattagca acaacccaat
      841 tccaattaaa tttccataaa acaatcctct tcctatcaaa taagtagcgt tatagataaa
      901 tatttttcta atactccaat tatttgctcc taatgattta agtattccta tcatttgggt
      961 tctttctaat attaaaacta gcaaagcaac aaccatatta attgtagaaa ctgctatcat
     1021 cacaattaaa attatcaaaa tattaaagtc gaaaagtttt aaccattcaa atatatagta
     1081 gtacttctca acgatagttt ttgaatctaa cgttgatgaa gtttcttcgt atatttcttt
     1141 acctttatat tcaatctctg aaaagtcttt tatataaact tcgaaagagc caatttgatt
     1201 aggcttccat ttgttgattc tttgtacgtg ccgaatatct cctaatatat atgtagcatc
     1261 aaattcttga aaacctgagt tgtaaattcc aacaatttta aagtttctaa gattatagcc
     1321 ttcgctggtt tctttcatga aataagtgac aaatttatca cctaacttca atcctagtct
     1381 attagcaagg tattgcgaaa tcattacttc attattcaaa tttttattta aaacaggtaa
     1441 tcttccttcg gtaatatatt ccgaaagatt gtctgactta taatcggttc caattccttt
     1501 aaaaatgatt ccttcaaatg cattttcggt tctaatcatt cctgctttac ttgcaactgc
     1561 ttgaacatga ttaattccat ctatattttt aaattcagga taaaagcttt gttttataga
     1621 aattggctcg gtactaacat cggattgatt atcattatag gaagaaataa taacatgtcc
     1681 gttgaacgcg gctacttttt ggcgtatttt ttgctgtaaa ccaatacctg tagctactga
     1741 aattatcatc ataatcattc ccaatgcaat tgcagttatg gcaattttta taattggcgc
     1801 agatatgcta cttttatggt ctttagccga aactaatttt tttgctgtaa aatattctaa
     1861 attcaactta aaatgatttc aaaattggtg ttcaaaaata cagttttatt atttgtcttg
     1921 ctaattcttt cttgcggtag ttcgattaag aaaatagaaa ataaagaaag gaaagaagtt
     1981 gtttcctttg aaaaaaaatt tcaagaaata aaaactggcg cagataactt cacttattat
     2041 ttaccattat taaaaggaaa aaaagttgga attgtaacca atcaaactgg gatattaagt
     2101 aataaaacac acttagttga ttttctattg gaaaaaaata taaatcttca aaaaatattc
     2161 gctccagaac atggttttcg tggcactgca gatgctggtg aacatgtaat cgatggaaaa
     2221 gacagcaaaa ctggtttacc tattgtttct ctttatggtg ataataaaaa accaaaaccc
     2281 gaacagttga aaggcattga tgttatgctt tttgatttac aagatgtagg cgtacgtttt
     2341 tacacttata tttcttcgct acattatatt atggaagctt gtgcagaaaa caatgttgaa
     2401 ttgattgttt tagacagacc aaatccaaat ggaagtattg ttgatggccc tattttagaa
     2461 aaagaaaatt ctagttttgt tggaatgcat gttattcctt tacttcatgg aatgacaatt
     2521 ggtgaatatg cacaaatgat tgatggcgaa aaatggttga aaaatggaat tcaatgcagc
     2581 ctaaaagtga ttccatgttt aaactataaa agagaaatgt catacagttt acctgtaaaa
     2641 ccttcaccaa atttaccaaa cgaccaatcc ataaatcttt acgctagttt gtgtttgttt
     2701 gaaggaacta ctataagtgt tggtcgtgga actgaaatgc agtttcaaat ttatggttcg
     2761 ccctatttac caaaaagtag ttttagcttt acaccaaaac caaattttgg tgcaaaagat
     2821 ccagtttata atggtaaact ttgctacgga gaaaacctga caactattcc aaaagtttca
     2881 aatcttgaat tgaaatggtt gatgaaagca tacaatgaaa ctcctgacaa aacaaaattt
     2941 ttcaatccgt ttttcacaaa gttagcagga actaaaaaac tgcaacagca aattgaagca
     3001 ggtattcctg aatttgaaat tagaaaatca tgggaaaaag gattgaatga ttttaaaacg
     3061 atgcgtaaaa aatatttgat ttacgattaa ttctttactt ctatacctgg atttaaccta
     3121 agcatttctt caataaattg aattttatct tttggagaaa tcattatttc atcgtgttta
     3181 ttataaacaa tagcaattct atctaatgat aatgctggtg aactcaaagg atttctagtt
     3241 ttatatattt ttctaattga tgaaatttct atttttttat ggacaataaa catgcttctt
     3301 actgttaagg tattgttttc aatacaataa taggtacttc tatacataaa aaccacaaaa
     3361 gctacagtta caacaataac tatccaacct gatttgttat gatttagtac ttcaacaatt
     3421 ccgaaataaa ttggaattaa aaacggaata atcaaccacc aatcgatttt tgaattatag
     3481 attttcattt tagaaaggca tttttttctt catttcttct acaatattat atgccgcagg
     3541 acaaattgaa acatttttaa gtgttaaatc agaaatttgc tgaaactttt ttctatctgt
     3601 atgtggaaat tctcggcacg cttttggacg aacatcatat atcatgcagt agttttcatt
     3661 gtctaaaaaa ctacacggta cattttgcaa cacataatct ttgtcttcat ctattcgaag
     3721 atattggtca ataaattgtt gtggtttttg tttaaaatgt ttagaaattc gctcaatatc
     3781 tgctgttgta aaaagtggtc cagttgtttt acaacaatta gcacattcca aacaattgga
     3841 ttttttaaac tctgcatcgt gcaaatcttg cattacataa tccaaattct tgggtgtcct
     3901 tttttttagt ttatcgaaat attttttgtt ttcgatatgc ttatctttgg ctagtttttt
     3961 taattcgtct aaatttggct ttatcatcaa tttttattct aattacaaat ttaaacaact
     4021 ttaaacttga aacctgaaac tatttaaaaa tgttagattt atttggtaaa gccattctcg
     4081 attttcaaac aaataatacg cccgaagata ttataactga aacatctatt tctgaagctg
     4141 atgaaataaa attacttcaa aagttagtaa aaacccgtaa
//
LOCUS       DAOOBH010000141         4130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_24213_length_4130_cov_5.191902, whole genome shotgun sequence.
ACCESSION   DAOOBH010000141 DAOOBH010000000
VERSION     DAOOBH010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24213_length_4130_cov_5.191902"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PK218_10690"
     CDS             complement(<1..1181)
                     /locus_tag="PK218_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPW99019.1"
                     /translation="MADIYKQKNNYKFAIANSNLAYKYATANNDVLNKSIALTSLGTI
                     YFELKKIPDAKDAFIKSINELNTLPLTSQLALNYYKLGLCAMTLEEYTLAEKYFSKAE
                     KIYRTLKIENTQLLLLQKGILYNAKGKKIEAAQIFNELISKPDDEDSYNTKAEALYQL
                     GSIEYNKNRSNLALNYLNRALALNTSSNNNIEQRIKILFLLSMTNDNLLNTSLAHTYL
                     KEHVLLRDSLLKLNNKRLAANDYISLKESERIDLFEQLSKENETQQRTNKFAKLISVL
                     AIALISILSLLSLSLYKNNIIRTQSNKLLQEKNSELEIAKEKAEKASQARADFLSTVS
                     HELRTPLNAINGITHLLIEENPKESQVHYLNSLKFSGNYLITFINEILEINRIESNNI
                     E"
     gene            complement(1311..2306)
                     /gene="gap"
                     /locus_tag="PK218_10695"
     CDS             complement(1311..2306)
                     /gene="gap"
                     /locus_tag="PK218_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPW99020.1"
                     /translation="MKQKTKIAINGFGRIGRNLFRLLLNHPTIEVVAINDLADNRTMA
                     HLIKYDSIHGILPYDTSFDENHIIVDGKSYLFFHEKEISNLKWINIDFVVESTGKFRT
                     FDEINKHIIAGAKKVILSAPSEVDEIKTVVLGVNEHILDGSEKIISNASCTTNNAAPM
                     IKVINELCGIEQAYITTVHSYTTDQSLHDQPHKDLRRARGASQSIVPTTTGAAKALTK
                     IFPEMDGKIGGSGIRVPVPDGSLTDITCYVKKQVTIEEINLAFKNASEKELKGIVAYT
                     EDPIVSVDILGNRNSCLFDAQLTSVIDKMVKVVGWYDNEIGYSSRIIDLISLLSK"
     gene            complement(2381..3250)
                     /gene="lipA"
                     /locus_tag="PK218_10700"
     CDS             complement(2381..3250)
                     /gene="lipA"
                     /locus_tag="PK218_10700"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPW99021.1"
                     /translation="MDSVLENNLPIGKPKWLKVKLPIGQKYTELRSLVDKYNLNTICA
                     SGSCPNMGECWGEGTATFMILGNICTRSCGFCGVKTGRPETVDWDEPEKVARSIKLMK
                     IKHAVITSVDRDDLKDMGSIIWFETVQAIRRMNPKTTLETLIPDFQGNEKIIDRIVAA
                     NPEVVSHNVETVRRLTREVRIQAKYDRSLQVLRYLKQQGIKRTKSGIMLGLGEKEEEV
                     IQTMQDLRNNNVDIVTIGQYLQPSKKHLPVKEYITPEQFEKYEKIGKELGFRHVESGA
                     LVRSSYHAEKHIL"
     gene            complement(3361..3564)
                     /locus_tag="PK218_10705"
     CDS             complement(3361..3564)
                     /locus_tag="PK218_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW99022.1"
                     /translation="MAIIVNLDVVMAKRKMSLNELSEKVDLTLSNLSILKTGKAKAIR
                     FSTLEAICKALDCQPGDILEFKN"
     gene            complement(3568..4104)
                     /locus_tag="PK218_10710"
     CDS             complement(3568..4104)
                     /locus_tag="PK218_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HPW99023.1"
                     /translation="MSKMNNLVFKILHVIAWIIFVGLSIEAGGLIVNFVFSVFKPEFV
                     GNLYQKLDLSKLHQQSEMVFFGMYSFILFISILKAILFYVVIQLLIKLDINKPFSVFV
                     SSKISQISQFTFWIGILSFIASQIAKSLQLQGYYIDKLGQFWVDSSAFILMAAIIYII
                     SLIFKRGIELQEENELTI"
BASE COUNT         1291 a          670 c          598 g         1571 t
ORIGIN      
        1 atttcaatat tattggattc aattcggtta atttctaata tttcattaat aaaagtgatt
       61 aaataatttc cggaaaactt caaagaattt agataatgta cttgactttc ttttgggttt
      121 tcttcaatca ataaatgtgt aattccgttg atagcattta aaggtgttct aagttcgtga
      181 ctaacagttg aaagaaaatc tgctctagct tgagatgctt tttcagcttt ttctttagca
      241 atttcaagtt cagaattttt ttcttgcaat aatttattgg attgggttct aataatattg
      301 ttcttataca aggataaact gagtaatgaa aggatggaaa ttaaagcaat tgcaagtaca
      361 ctaatcaatt tagcaaattt gttagtcctt tgttgcgttt cattttcttt acttaattgt
      421 tcaaataagt caatgcgttc gctttctttt aaggatatat aatcgtttgc tgcaagacgt
      481 ttattgttca atttcaacaa actatctcta agtaatacat gttcttttaa ataagtatgc
      541 gctaatgatg tatttaataa attatcattt gtcatactta ataaaaacaa tatttttatg
      601 cgttgttcaa tattattgtt actactagta ttaagtgcta aggctctgtt tagataattt
      661 aaagccaaat tactcctgtt tttattgtat tctattgatc ctaattgata taaagcttca
      721 gctttagtat tgtaagagtc ttcatcatca ggtttagaaa tgagttcgtt aaatatttgt
      781 gctgcttcaa tttttttgcc tttagcatta tataaaatac ctttttgcaa aagtaacaac
      841 tgtgtatttt caatttttaa cgttctgtaa attttttcag ctttgctaaa gtatttttct
      901 gccaaagtgt attcttccaa agtcatggca caaagaccaa gtttgtagta gtttaaagcg
      961 agttgtgatg tcagaggcag tgtgttaagt tcattaatac tttttatgaa tgcatctttg
     1021 gcatctggaa ttttctttaa ctcaaaataa atggttccaa gtgaagttaa ggcaatagat
     1081 ttattaagaa catcattatt tgcagtagca tatttataag ccaaattgga gttagctatg
     1141 gcaaatttgt aattgttttt ttgtttatat atatctgcca attttatata ataagcaacg
     1201 ctatcgattt tttcgacagt ttttttttga ccaaaagcta tggtcgtaac tagtaaaaaa
     1261 ataaaaattc ctctcaactc attatagttt aagagaataa atatatatga ttatttactt
     1321 aaaagcgaaa ttaaatcgat tattctcgat gaataaccaa tttcattgtc gtaccaacct
     1381 acaaccttaa ccattttgtc aattaccgat gttaattgtg catcaaataa acatgaattt
     1441 ctgttcccta aaatgtcaac ggatacaatt ggatcttcgg tataagcaac aattcctttc
     1501 aattcctttt cagaagcatt cttaaaagca agattgattt cttcgatagt aacttgtttt
     1561 tttacataac aagtaatatc ggttaatgaa ccatcaggaa ctggaacacg aatgccgctt
     1621 ccaccaattt ttccgtccat ttctggaaat attttggtca gcgcttttgc tgctccagtc
     1681 gtagtgggta caatggattg acttgcccca cgagctcttc tcaaatcttt atgtggttgg
     1741 tcgtgtaaac tttggtcagt agtgtaggag tgaaccgtgg taatataggc ttgttctata
     1801 ccgcaaagtt cattgatgac ttttatcatc ggtgcggcat tatttgtagt acaactagca
     1861 tttgagatta ttttttccga gccatcaaga atatgctcat taacacccaa aactactgtt
     1921 tttatttcat ctacttcact aggtgccgat agaattactt ttttggcacc agcaataatg
     1981 tgtttattaa tttcatcaaa agttctgaat tttccagtcg attctactac aaaatcgatg
     2041 ttaatccatt ttagattaga aatttctttc tcatgaaaaa aaagatatga ttttccatct
     2101 acaataatgt gattctcatc aaacgaagta tcataaggta aaataccatg aatactgtcg
     2161 tatttaatca aatgtgccat tgttcggttg tcggcaagat cattgatggc aacaacttca
     2221 atagtagggt gatttaataa caatcggaat aaatttcttc caattcgtcc aaaaccatta
     2281 atggcaattt ttgttttttg tttcaagttt aaggttgttt aatttggctt aaaatttcca
     2341 ttttccattt tttttcactt cgtacctcga acctaatagc ttataaaata tgtttttctg
     2401 catgatacga tgaacgaacc aatgcaccac tttcaacatg tcggaagccc aactctttgc
     2461 ctattttttc atatttctca aactgttctg gagtaatata ttcttttaca ggtaaatgtt
     2521 ttttactagg ttgcaaatat tgacctatag ttacaatatc aacattatta ttgcgcaagt
     2581 cttgcatggt ttgaataact tcttcttctt tttcgcccaa tccaagcatt attcccgact
     2641 tagttctttt aattccttgt tgttttaaat agcgcaaaac ttggagactt ctatcatatt
     2701 tagcttgaat acgaacttct cttgtcaatc ttcgtacggt ttctacatta tgcgaaacta
     2761 cttcaggatt tgcagcaaca attctatcta tgattttttc gtttccttga aaatctggaa
     2821 ttaaagtttc taaagtggtt ttaggattca ttcttcggat ggcttgcaca gtttcaaacc
     2881 agataatcga acccatatct ttcagatcat ctctatcaac actggtaata acagcgtgtt
     2941 tgattttcat taatttaatc gaacgtgcta ctttttcggg ttcgtcccaa tctactgttt
     3001 ctggtcttcc cgtttttact ccacaaaatc cacatgaacg tgtgcaaata tttcccaaaa
     3061 tcataaacgt agctgttcct tctccccaac attcacccat gtttgggcaa cttcccgaag
     3121 cacaaattgt gtttaggtta tatttgtcaa ctaaagagcg aagttcggta tatttttgtc
     3181 caataggcag ttttactttc agccactttg gttttccaat aggtaaatta ttttcgagta
     3241 ctgaatccat aatctttttt taacagtgca aagataaaga atgtagttag aagtatagtt
     3301 gatttttaaa ttttgtgatt attatttaga cctagaggtt tcctaatgca attatttttt
     3361 ctaatttttg aattccaaaa tatcgcctgg ctgacaatct aatgctttgc aaattgcttc
     3421 taaagtgcta aatctaatag cttttgcttt accagttttt aaaatagaaa gattcgataa
     3481 agttaaatct actttttctg aaagttcgtt taatgacatt tttcgtttgg ccatcacaac
     3541 atcaaggttt actattatcg ccatggctta tattgttaat tcgttttctt cttgcagttc
     3601 aattcctctt ttaaaaatga gagaaataat ataaataata gctgccatta aaataaatgc
     3661 agaactatct acccaaaatt gacctaattt atcaatataa tagccttgaa gttgtaaact
     3721 tttggcaatt tgacttgcta tgaaactcaa tattccaatc caaaatgtaa actggctaat
     3781 ttgtgaaatt ttagatgata caaatacgct gaatggtttg tttatatcta acttgataag
     3841 taattgtatt acaacataga aaagtatagc ttttaagatt gatataaaga gtataaaact
     3901 atacataccg aaaaaaacca tttcactttg ttgatgcaat ttacttaaat caagtttttg
     3961 atatagattg cctacaaatt ctggtttaaa aacactaaaa acaaagttaa caattagacc
     4021 acctgcttca atacttaaac cgacaaagat tatccatgct attacatgta aaattttaaa
     4081 tactaagttg ttcatttttg acataattat cttttttaaa atttacaaag
//
LOCUS       DAOOBH010000142         4122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_24273_length_4122_cov_4.817802, whole genome shotgun sequence.
ACCESSION   DAOOBH010000142 DAOOBH010000000
VERSION     DAOOBH010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24273_length_4122_cov_4.817802"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..260)
                     /locus_tag="PK218_10715"
     CDS             complement(30..260)
                     /locus_tag="PK218_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99024.1"
                     /translation="MSKKTQAFLYNLICFAILFILFRFLIEQYTHLTGYFIPITAFVV
                     GTLLAPKFQAAKYQGEEKIFIRWIFMKGVKQF"
     gene            complement(282..1016)
                     /locus_tag="PK218_10720"
     CDS             complement(282..1016)
                     /locus_tag="PK218_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HPW99025.1"
                     /translation="MKHILKYTFSLVLMLISFVTLAQEQTPKTDSIVPKKERYGIRLG
                     VDLYKLTRSIYDKDYQGVEFVGDYRLTRKHYLAAEIGNENKTVDEDQLNFTTKGSYIK
                     VGFDYNSYENWLTMENQIYVGLRYGFSTFNQTLNSYSIYNNNQYFGPSELTVSGEKFD
                     GLSAQWLEVVAGVKAEVFKNVFVGFSLRINRLVNQKQPNNFENLYIPGFNRTYNGDFG
                     VGFNYTVSYFIPLYKSTVKTKKKKNN"
     gene            complement(988..1485)
                     /locus_tag="PK218_10725"
     CDS             complement(988..1485)
                     /locus_tag="PK218_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HPW99026.1"
                     /translation="MKKIILLLFIAISFSSCEKDDVCADDTTPKLVIDFFDISNPSNP
                     KNFINLKVTAVGQTEPFGTFTSGNTIVIPLRISENSTKYSFILNSNDTVNLNEDFLEF
                     NYTTQNIFVSRACGYKTIFEFNNDGTGVIKTDAAIPDGIWMQNVVKVTNSITTENETH
                     LKIYF"
     gene            complement(1510..2919)
                     /gene="rlmD"
                     /locus_tag="PK218_10730"
     CDS             complement(1510..2919)
                     /gene="rlmD"
                     /locus_tag="PK218_10730"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPW99027.1"
                     /translation="MRRKETKKIIFENITVLDAGAKGVSVAKAPEGQVIFIPNVVPGD
                     VIDVQTLKKRKSYYEGRAIKFHKLSVHRTEPVCQHFGTCGGCKWQNMKYEQQLFYKNQ
                     EVYNNLKRIGKIELPDFEPILGSEKQFFYRNKMEFGFSDSRWLTNEEIQSGESFQKNA
                     LGFHIPRMWDKILDIEKCHLQEDPSNALRNSIRDFATEKGMTFFNARNHTGLLRTLMM
                     RTASTGEIMVLIQFFEEDKTNRELLLDFIYEKFPNITSLQYVINSKANDTLYDQDIIL
                     YKGRDYILEEMEGLHFSINAKSFYQTNSDQAYELYKITREFAGLTGNELVYDLYTGTG
                     TIAQFVSKLAKKVIGVEAVPEAIFDAKENAKRNEITNCEFYVGDMKNVFNEDFIASHG
                     KPDVIITDPPRDGMHKDVVQQILNIAPERIVYVSCNSATQARDLALLDEKYKVTRVRP
                     VDMFPQTHHVENVVLLEKK"
     gene            complement(3103..>4122)
                     /locus_tag="PK218_10735"
     CDS             complement(3103..>4122)
                     /locus_tag="PK218_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW99028.1"
                     /translation="KRRTSKNSITVHGARENNLQNIDVTFPLESLTVITGVSGSGKST
                     LVKKILYPAMQKKITSMSDKAGQFSDLSGSFSHVKHIEYVDQNPIGRSSRSNPVTYIK
                     AYDDIRDLYSKQKLSKIRGYQAKHFSFNVDGGRCETCKGDGSINVEMVFMADVELPCE
                     TCNGKRFKKEVLEVTFENKNIDDILTMTVDDAISFFSEYKQTKIIQKLQPLQDVGLGY
                     VQLGQSSSTLSGGEAQRIKLASFLVKGTTKDKALFIFDEPTTGLHFHDIKKLLASFDA
                     LIDKGHSIIVIEHNLDLIKCADWIIDLGPEGGENGGKLLAIGTPEEIVKNKKSITGSY
                     LKEKL"
BASE COUNT         1299 a          733 c          635 g         1455 t
ORIGIN      
        1 atttactaaa aattcaacaa atacttgctc taaaattgtt ttacgccttt cataaaaatc
       61 caacgtataa atattttttc ttctccttga tactttgccg cctgaaattt tggtgctaat
      121 aaagtaccca caacaaaagc agttatcgga ataaaatatc ctgttaaatg cgtatattgt
      181 tcaatcaaaa aacgaaacaa aataaataaa atggcgaaac aaattaagtt atataggaag
      241 gcttgtgttt tttttgacat gattatttaa ttacgattta attaattatt ttttttcttt
      301 tttgttttga ctgtcgattt atacaagggt ataaaatatg aaaccgtata attaaatcca
      361 actccaaaat caccattata agttctattg aatccaggaa tgtaaaggtt ttcaaagtta
      421 ttaggttgtt tttgattaac caatcgattg attcttaaac taaaaccaac aaaaacattt
      481 ttgaagactt ccgctttcac tccagctaca acttcaagcc attgagctga tagaccatca
      541 aatttctctc ctgatactgt caattcactt ggtccaaaat actgattgtt gttataaata
      601 gagtaggagt tgagtgtctg attgaaagtg ctaaacccat aacgcaaacc tacatagatt
      661 tggttttcca tagtaagcca attttcataa ctattatagt caaaacctac tttaatatat
      721 gaacctttgg tagtaaaatt taattgatct tcatcaactg ttttattttc gtttcctatt
      781 tcagcagcca agtaatgttt tctcgtcaat ctataatctc caacaaattc aactccttga
      841 tagtctttat cataaataga gcgtgtcaat ttatacaaat caactccaag acgaatacca
      901 tagcgttctt tcttgggtac aatcgaatct gttttcggag tttgttcttg tgccaatgtt
      961 acgaatgaaa tcagcatcaa aaccaaacta aaagtatatt ttaagatgtg tttcattttc
     1021 tgttgtaata gagttggtta ctttcactac gttttgcatc caaattccat ccggaatagc
     1081 ggcatctgtt ttaattacgc cagtgccatc gttattaaat tcaaatatgg ttttatagcc
     1141 acaagctctc gaaacaaaaa tgttttgagt ggtatagttg aattctaaaa agtcctcatt
     1201 caaattgacg gtatcattac tgtttaatat gaaactatat ttagtagagt tttcagaaat
     1261 tctaagtgga attacaattg tgttcccaga agtaaaagtg ccaaatggtt ctgtttgacc
     1321 aacagctgta acttttaaat tgatgaaatt ctttgggtta gaaggatttg aaatgtcaaa
     1381 aaaatcgata accaattttg gagttgtatc atctgcacaa acatcatctt tttcacaact
     1441 tgaaaaggaa attgcaatga ataatagaag aatgattttt ttcataaatt ctttgtactt
     1501 gatatttagt tacttttttt ccaaaagtac aacattttct acatgatgtg tttgtggaaa
     1561 catatccact ggtcgaacac gagttacttt atatttttcg tctaataaag ccaaatctct
     1621 tgcttgtgtt gctgaattac aacttacata aactattctc tcaggtgcaa tattcaatat
     1681 ttgttgcact acatctttat gcattccatc tcttggagga tcagtaataa taacatctgg
     1741 ttttccgtgt gacgcaataa aatcttcgtt gaaaacgttt ttcatatcac ctacatagaa
     1801 ctcacaatta gtgatttcat tacgttttgc attttcttta gcgtcaaata ttgcttctgg
     1861 aactgcttct acaccaatta cttttttggc caacttcgat acaaattgtg caatcgttcc
     1921 tgtgccagta tataaatcat aaactaattc attgcctgtt aacccagcaa attctctagt
     1981 aattttatac aattcatacg cttggtccga attggtttgg taaaacgatt ttgcattgat
     2041 gctaaagtgt aaaccttcca tttcttccaa tatgtagtca cgacctttat ataatattat
     2101 atcttggtca tatagtgtat cgttcgcttt actattaatt acatattgta atgaggttat
     2161 gtttggaaac ttttcgtaga taaaatccaa aagtaattct ctattggttt tatcttcttc
     2221 aaagaattga atcaatacca tgatttcacc agttgaagct gttcgcatca ttaaggtgcg
     2281 taataaacct gtatgatttc gagcattaaa aaaagtcatt cctttttcag tagcaaaatc
     2341 acgaattgaa tttcttaagg catttgatgg atcttcttgc aagtggcatt tttcaatatc
     2401 aagaatttta tcccacattc ttggaatatg aaaccctaga gcattcttct gaaacgattc
     2461 accagattga atttcttcat tggtcaacca acgagagtct gaaaaaccaa actccatttt
     2521 gtttctataa aaaaactgtt tttccgaacc caatattggt tcaaagtcgg gcaattcaat
     2581 tttgccaatg cgtttcaggt tattatatac ttcctgattt ttataaaaca actgttgctc
     2641 atacttcatg ttttgccatt tgcaaccgcc acatgtgcca aaatgttggc aaacaggctc
     2701 ggtgcgatgt actgataatt tatgaaattt tatggctctt ccttcataat acgatttgcg
     2761 tttcttcaat gtttgcacat ctataacatc gcccggaacc acattaggaa taaatataac
     2821 ttgaccttct ggtgctttgg ctaccgaaac tcctttagca cctgcatcaa gtacggtaat
     2881 attttcaaag attattttct tcgtttcttt tcttctcata gcgcaaagat ataaaatgaa
     2941 gtatgaaatt ataggcaaca aatacgaaat aaaatgataa ttttaatgaa tttgaaaatg
     3001 aatataatag tttgtctaag tgaaaataag acgaaaaaac attattttat tttttgcact
     3061 ataaattttt gataagttaa taaaatgagt ctaggctgat aattacagtt tctcttttaa
     3121 ataacttcca gtaatcgact ttttattctt cacaatctct tcgggagttc caatggcaag
     3181 tagctttcca ccattttcac cgccttcagg accaaggtcg ataatccaat cggcacattt
     3241 aattaaatcg aggttgtgct caatgacaat aatcgaatgt cctttgtcta tcaaagcatc
     3301 aaaggaagct aacagtttct tgatgtcgtg aaaatgcaac ccagtagttg gttcgtcaaa
     3361 aataaaaagg gctttgtctt tagtagttcc tttaactaag aaggaagcca acttgattcg
     3421 ttgtgcttcg ccgccagaaa gagttgagga ggattgtccg agttgcacat agcccaaacc
     3481 aacgtcttgc aatggttgta gtttttggat aatcttggtt tgtttgtact ccgagaagaa
     3541 agaaatggca tcatcaacgg tcatggtcaa gatatcatcg atatttttgt tttcaaaagt
     3601 aacttcgagc acttcttttt tgaagcgttt tccgttgcag gtttcacaag gcaattcaac
     3661 atcggccata aagaccattt caacattgat acttccatca cctttgcagg tttcacagcg
     3721 accaccatct acattaaaag aaaaatgttt ggcttggtat cctcgtattt tagaaagctt
     3781 ttgcttggaa tacaaatcgc gaatatcatc atatgcctta atatatgtaa ctggatttga
     3841 tcgtgagctt ctaccgatag gattttggtc aacatattcg atgtgtttta catgagaaaa
     3901 acttccagac aagtctgaaa attgtcccgc tttatcactc atactcgtga tttttttctg
     3961 catggcggga tacaatatct ttttgaccaa agtgcttttg ccactacccg aaacaccagt
     4021 aatgacagtt aagctttcta gaggaaaagt tacatctata ttctgaagat tattttctct
     4081 agctccatga acggtaatgc tgtttttgga agtcctacgt tt
//
LOCUS       DAOOBH010000143         4114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_24331_length_4114_cov_3.858832, whole genome shotgun sequence.
ACCESSION   DAOOBH010000143 DAOOBH010000000
VERSION     DAOOBH010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24331_length_4114_cov_3.858832"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2752
                     /gene="ccsA"
                     /locus_tag="PK218_10740"
     CDS             <1..2752
                     /gene="ccsA"
                     /locus_tag="PK218_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPW99029.1"
                     /translation="YVDGDYEGGIKRRTVEKSLLLSPVADNYFSINKKFGTIPFEVEY
                     KDYVLDAKETIKADDKGVLYLKLVESGDGTRHEHYLKEGEIQNIHNVLFALNKPTNGA
                     VNINTINNTIVTPFDGQFMRMADKLEGKVIKDSVQPLMYRSLYNVGGSQFVLPEAAIK
                     GVKSFEASGNYKPKQHDDALTVVVKSGGETKEVTLIGAKGKQGEPVVVKLGKLDFTLM
                     FGSKVYELPFKIKLNDFIADKYPGTEKSYSAFESQVTVIDSAKKFDARIYMNHILDYK
                     GFRFFQASFSPDEKGTVLSVNHDFWGTTITYIGYFLLFFCMMAILFVKNTRFAELKRK
                     LETVKKKKAKLLSILLLMMSCTIGFSQENHQHKQTNFNYKDSILKYKVPLIQADRFGR
                     LVIQDDGGRMKPINTFSSELLRKVSHSDTYEDMNADQVFISMKQFPFAWMEIPLVYIK
                     KDNDSIRKILGVEAKQKLIPFAAFFDKEGNYKLSPYLDEAYKTANPNQFEKDFVETDK
                     KINLLNSALFGSILKIYPIPGDKNNKWVSNLELNQPTGTALDTIKAAFPLYLNYLAEA
                     NSSKNYTMANTMLTGLEKFQLKYGSKVMPSQEKINYEVMYNKYDVFKKLPYWYISLAI
                     LMFVLVIIQIFKDRKVLRISINVLHLLIGLCFALHTVGLIARWYISGHAPWSNAYEAI
                     VYVAWATMFFGLAFDIKSKLTVASSAFVTAMILTAAYANWIDPEIANLQPVLNSYWLM
                     IHVAVIVASYGPFALGFILGFVSLLLMFFTTEKNKVKMDLNIKELTYINEMALTVGLV
                     MLTIGNFLGGQWANESWGRYWGWDPKETWALISIMVYAFVIHARFVPGMKNRWIFNVM
                     AMFAFISILFTYYGVNFHLVGLHSYASGEAKSLDWIYEMILGVSIVGAISYPKFRKYY
                     KN"
     gene            complement(2812..3279)
                     /locus_tag="PK218_10745"
     CDS             complement(2812..3279)
                     /locus_tag="PK218_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99030.1"
                     /translation="MKKLLLLTVIVLAISACKNKTEVKNNTVAAPKTVTLNPNTSIEK
                     GCYRYSNGGSVVAMEITENENQVAGTLEIAYAEKDASKGTFAGTMNGDKLMATYTFLS
                     EGKVNTREIAYLLKNNQLIEGYAEMDEKGTKFKDTNAIEYTSTMPLSRVDCNQ"
     gene            complement(3295..4053)
                     /locus_tag="PK218_10750"
     CDS             complement(3295..4053)
                     /locus_tag="PK218_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HPW99031.1"
                     /translation="MQNSIKTSMLILVATTISCNTMKSTPSEVKKQQDDSQVYFKANG
                     TEPFWGLTISEKQIKLFTVQDTIVMPHQKPIQAMDSNVKLYKLKSDTDEMSVQIIQTE
                     CTNDMSGEKFPYTISINYKKNKELVMHQLKGCGKYNTDYRLNDLWVLEKLNGKEMEKS
                     FFKDNLPSLEINSETNSFSGFAGCNGMSGQLFYEKELLRFTKIITTMMYCGDTNKERE
                     FLKALNSATKYTIENNRLTLSNPSKELMVFKKID"
BASE COUNT         1268 a          696 c          766 g         1384 t
ORIGIN      
        1 ttatgttgat ggagattatg aaggcggtat taaaagaaga accgttgaga aatcattatt
       61 gctttcgcca gttgcagaca attacttttc tattaataaa aaatttggca caataccttt
      121 cgaagttgaa tataaagact atgttttaga tgccaaagaa accattaagg cagacgacaa
      181 aggtgtgctg tatttgaagc tagttgaatc aggtgatggt actcgacatg aacattatct
      241 taaagaaggc gaaattcaga acatccacaa tgtacttttt gctctaaaca aaccaaccaa
      301 tggtgccgta aacattaata ctatcaacaa taccatcgtt actccatttg acggacagtt
      361 tatgcgaatg gcagataaat tggaaggaaa agttatcaaa gattctgtgc aaccattgat
      421 gtatcgctcg ctgtacaatg ttggcggttc ccaatttgtg ttaccagaag cggcaataaa
      481 aggtgtaaaa tcctttgaag cttcaggtaa ttataaacca aaacaacatg acgatgcact
      541 aaccgttgtt gtaaaatcgg gtggcgaaac gaaagaagta accttaattg gtgcaaaagg
      601 taagcaaggt gagcctgtag ttgtcaaact tggtaaactc gatttcactt taatgtttgg
      661 cagcaaagtc tatgagcttc cattcaagat aaaactaaat gattttattg ccgacaaata
      721 tcctggtacc gaaaaaagct attctgcttt cgaaagccaa gtaacggtaa ttgattctgc
      781 caaaaaattt gatgcacgca tctatatgaa ccacattcta gactacaaag gatttcgttt
      841 ttttcaagca tcgttttctc ccgatgaaaa aggaacagta ttgtcggtaa accacgattt
      901 ttggggaaca accatcactt acattggcta tttcttgtta tttttctgca tgatggcaat
      961 cctttttgta aaaaacacac gctttgccga gctaaaacga aaactagaaa ccgttaaaaa
     1021 gaaaaaagcc aaattgcttt ccattctttt gttgatgatg agttgcacaa ttgggttttc
     1081 gcaagaaaat catcagcata aacaaactaa ttttaactac aaagattcga ttttaaagta
     1141 taaagtaccg ctgatacaag ccgacaggtt cggacgattg gtcattcaag acgatggtgg
     1201 aagaatgaaa cctatcaata ctttttcttc cgaattattg cgaaaagtga gtcatagtga
     1261 tacttacgaa gatatgaatg cagaccaagt attcatttcg atgaaacaat ttccgtttgc
     1321 ttggatggaa attccgttag tatatattaa aaaggacaac gacagcatcc gaaaaattct
     1381 tggtgtagaa gcaaaacaaa aactcattcc gtttgcagca ttttttgata aagaaggcaa
     1441 ctataaattg tcgccctact tggatgaagc ctacaaaacc gccaatccaa atcaatttga
     1501 aaaagatttt gtagaaaccg acaagaaaat caacctatta aattcggctt tgttcggaag
     1561 tattttgaaa atctatccaa ttcctggcga taagaacaac aaatgggttt ccaacctcga
     1621 actgaaccaa cctacaggaa ctgcattaga cactatcaaa gctgcctttc cgttgtatct
     1681 aaattatttg gcggaagcta acagttctaa aaattatacc atggcaaaca ccatgcttac
     1741 tggattagag aaattccaat tgaagtatgg aagcaaagta atgccaagtc aagaaaaaat
     1801 caactatgaa gtgatgtaca acaagtatga tgtgtttaag aaattacctt attggtatat
     1861 ttctctagcc attttaatgt tcgtcttggt aatcattcaa atttttaaag accgcaaagt
     1921 gttgagaatc tcaattaatg ttttgcacct tctaattggg ttgtgttttg ccttgcacac
     1981 cgtcgggtta atcgcccgtt ggtacatttc gggtcatgcg ccatggagca acgcctacga
     2041 agccatcgtt tatgtagctt gggcaaccat gttcttcgga ttggcattcg acataaaatc
     2101 aaaactaacc gtagcatcat cggcatttgt aacggcaatg atattaaccg cagcatatgc
     2161 caattggatt gatcctgaaa tagccaattt gcaaccagtg ttaaattcct attggttgat
     2221 gattcacgta gctgtgattg tggcaagtta tggtccgttt gcacttggat ttattcttgg
     2281 ttttgtatcg cttttgctga tgttctttac caccgaaaag aacaaagtaa aaatggattt
     2341 gaacatcaaa gaattgacct atatcaacga aatggcgcta acagtgggct tggtgatgct
     2401 taccattggt aattttcttg gtggacaatg ggcaaacgaa agctggggaa gatactgggg
     2461 atgggatcca aaagaaacct gggcactaat tagtatcatg gtatatgcgt tcgtaattca
     2521 tgcgcgtttt gtgcctggaa tgaaaaaccg ttggattttt aacgtaatgg caatgtttgc
     2581 atttatctcc attttgttta cctattatgg tgtaaacttt catttggttg ggttgcactc
     2641 ctatgctagt ggtgaagcga aatccttaga ttggatttat gaaatgatac ttggtgtttc
     2701 aattgttggt gctatatcct atccaaaatt caggaaatat tataaaaatt aaagatactt
     2761 gacggactta aacacaattt gtcaaatatc ttaaattgta ttttttagcg attattggtt
     2821 acaatctact cttgataaag gcatagtaga agtatattct atagcgttgg tatctttaaa
     2881 cttagtgcct ttttcatcca tttcagcata gccttctatg agttggttgt tttttagaag
     2941 ataggcaatt tctcttgtat ttacttttcc ttccgacaaa aaagtataag ttgccattag
     3001 tttatcacca ttcatagttc ccgcaaatgt tcctttggaa gcatcttttt cggcgtaggc
     3061 aatctctaat gtacctgcca cttgattttc gttttcagtt atctccattg ctactacgga
     3121 accgccatta ctataacgat agcatccttt ttcgatactt gtatttgggt ttagtgtaac
     3181 tgttttaggt gcagctacag tattgttttt aacttcagtt ttatttttac aggcggatat
     3241 tgcaagaacg attacggtta gtaggagtag ctttttcata atagtcatat tttgttagtc
     3301 aattttttta aaaaccatta attcttttga aggattggat agtgttaatc gattgttctc
     3361 aatggtatat tttgttgcac tgtttagtgc ttttaaaaat tctctttctt tattggtatc
     3421 gccacagtac atcatggttg taataatttt ggtaaaacga agtagttctt tttcataaaa
     3481 tagttgtcct gacatgccat tgcaaccagc aaaaccagaa aaggaattgg tttcgctatt
     3541 gatttctagc gatggtagat tgtctttaaa gaaagatttt tccatttctt ttccgtttaa
     3601 tttctccaaa acccaaaggt cgtttagtct ataatcggta ttgtattttc cacaaccttt
     3661 aagttgatgc atcactaatt ctttattttt tttataatta atagatattg tgtatggaaa
     3721 tttttcgcct gacatgtcat tggtacattc ggtttgaatg atttggacag acatttcatc
     3781 ggtatccgat tttagtttat acaactttac attgctgtcc atggcttgta taggtttttg
     3841 atgtggcata acgatggtgt cttgcacggt gaaaagcttt atttgttttt cggaaatggt
     3901 taaaccccaa aaaggttcag tcccattggc tttaaaatac acttgactat catcttgttg
     3961 ctttttaact tcacttggtg ttgatttcat agtgttacaa gaaatagttg tcgctactaa
     4021 gattagcatg cttgttttga tactgttttg cataatttat taatttttta gcaatatgca
     4081 caaagatact ttttttattt ttttaaagaa taca
//
LOCUS       DAOOBH010000144         4080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_24580_length_4080_cov_4.660870, whole genome shotgun sequence.
ACCESSION   DAOOBH010000144 DAOOBH010000000
VERSION     DAOOBH010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24580_length_4080_cov_4.660870"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1848
                     /locus_tag="PK218_10755"
     CDS             <1..1848
                     /locus_tag="PK218_10755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPW99032.1"
                     /translation="AEQDFIKAGFTVVGSENNENKLLSFLKENYPSVIKDNEIDLQEL
                     KAIAGLPIDEKVNGYGLNFVGRNFARAKYAQKTEKELRLNNTLSKNIDTTENLVLKGD
                     NLDSLKILKNHYSGKIKCIYIDPPYNTDKDEFVYPDKFDKEEAEVLGLANLSESDFAR
                     MDFSFKTKKSHNGWLAFMYPRLLLARDLLSNDGAIFISIDDNEQANLKLLCDDVFGEE
                     NTEVMIWRKSGIGRDGKMKNTTTFRNDHEYVIVAHKLNKNLNKSYEKPQWENEYGNPD
                     NDPRGNYKTGSISRKEEASNPNSENYYSVTSPTGKVFLRQFEISINDFQKLVEDNRIY
                     WGKNGDAVPSQKIFVDEKRQVTTSSLIDNKTMTTTIGSKEIEELLNTENLGADIRPKP
                     SDLIVKLLQIGTEENDIILDFFAGSGTTGHAIMQLNAEDGGNRKFILCQIDEPIKEDK
                     PAYKFCIENNLPPVISSITIERLKRAGEKIANAIEADNSKTGLFEEDKKQVPDIGFKV
                     FDSVEAPKLKVDDKGQISITENEMDTLSRIYNMIFTVGLDEPTQVPEEVVKDCIYRIG
                     NNYYITNSEKITGDDFANAIKNGKVFVDGWTASLNGTLQSYKEDVKIVF"
     gene            2163..3038
                     /locus_tag="PK218_10760"
     CDS             2163..3038
                     /locus_tag="PK218_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HPW99033.1"
                     /translation="MAKKNTQQPELIITKLTIDKCEFKNALNERIILGEEIYNRTIQT
                     SSEFKKMMSDFSAWTDYNTEYLKQSFNNPHNEYKRAYEDAGHSFFVSLGGNNKNPLQK
                     KKEFIFAKIENLKKLVAKADLLKTSLTSTVSSTPQQAKVAVAMNEVFIVHGQDETAKT
                     KTARFIEKLGLKPIILHEQASGSKTVIEKIEAYSNVGFGIVLYTPCDIGAKNEKEPNF
                     KNRARQNVVFEHGFLIGKIGRENVCALVKGEIETPNDISGVVYVEMDDGEAWHVKIAR
                     ELRNSGYEIDMNKVI"
     gene            3044..3745
                     /locus_tag="PK218_10765"
     CDS             3044..3745
                     /locus_tag="PK218_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99034.1"
                     /translation="MGKFDWTYIGTLLSVTFGWFLNELSQWFRTRKEDRKIKKQVLFY
                     LLETHFTFNKLDTSEITNLLSQKVLERISKNEQPDEAKQFINQLYAGIISGFVEDNVA
                     ESLEETEEHYTKSISELSKLDPITAYQLNGKNKIFQVFDLLHDYYDKVKETLPNEPGL
                     NEQIKLTIETIKPEIIKDAISDLEDKIRDIALSINLWTLYRARRAVNNTKTKIRKDDE
                     KKIDELLNMVMPKMT"
BASE COUNT         1596 a          643 c          742 g         1099 t
ORIGIN      
        1 gcagaacaag attttataaa agcaggattt acagtggttg gctcagaaaa caacgaaaac
       61 aaattattaa gttttttaaa agaaaactac ccaagcgtaa ttaaggataa cgaaattgac
      121 ttgcaagagc tcaaagctat tgcaggttta cctattgatg aaaaggtaaa cggttacggg
      181 ttaaactttg taggtaggaa ctttgcccga gccaaatatg ctcaaaagac agaaaaggag
      241 ttacgcctga ataatacatt aagtaaaaat attgacacaa ccgaaaactt agtattgaaa
      301 ggcgacaacc tcgacagctt aaaaattcta aaaaatcatt acagtggaaa aataaagtgt
      361 atttacatag acccacccta taatactgat aaagatgaat ttgtttatcc cgacaaattt
      421 gacaaagaag aagcggaagt tttgggattg gcaaacttga gcgaaagcga ttttgcacga
      481 atggatttta gcttcaaaac gaaaaagagc cacaacggtt ggttggcatt tatgtatcca
      541 cgcctattat tggcaagaga ccttttgagc aatgatggag caattttcat aagcattgat
      601 gacaatgaac aagcaaacct aaaactgctt tgtgatgatg tttttgggga agaaaatacc
      661 gaagtaatga tttggcgtaa gagcggcatt ggtagagatg gcaaaatgaa gaatacaaca
      721 acttttagaa acgaccacga atatgtgatt gtagcacata aattaaataa aaacctaaat
      781 aaatcttatg agaaacctca atgggaaaat gaatatggca atcctgacaa tgacccaaga
      841 ggtaattata aaacaggttc tatttcacgt aaagaagaag cctcaaaccc aaattcagaa
      901 aactattatt cagttacttc tccaactggt aaagtatttt taagacagtt tgaaatttct
      961 ataaatgatt ttcaaaaatt agttgaagat aatagaattt attgggggaa aaatggtgat
     1021 gcagtgccat ctcaaaaaat atttgtagat gaaaaaagac aagtaacaac atcatcttta
     1081 attgataata aaacaatgac taccacaata ggtagtaaag aaattgaaga attattaaat
     1141 acagaaaact tgggtgccga tattagacct aaaccatctg acttaattgt aaaattattg
     1201 caaattggaa cagaagaaaa cgacatcatc ctcgacttct tcgcaggtag cggcaccaca
     1261 gggcacgcca ttatgcaact aaatgcagaa gatggtggca atagaaaatt tatcttgtgc
     1321 caaatagatg aaccaataaa agaagacaaa cctgcttaca agttttgcat tgagaacaat
     1381 ttgcctcccg taatttcaag cattacaata gaacgcttga aaagagcagg agaaaaaatt
     1441 gccaatgcca tagaagctga caacagcaag acaggattgt ttgaggaaga caaaaaacaa
     1501 gttcctgata ttggttttaa agtgtttgac agtgtagaag caccaaaact gaaagttgac
     1561 gacaaagggc aaatttcaat aactgaaaat gaaatggata cgttaagtcg catttacaat
     1621 atgattttta ccgttggttt agacgaaccg acacaagttc ccgaagaagt agtgaaagat
     1681 tgcatttacc gaattggaaa caactactac atcaccaaca gcgaaaaaat aaccggcgat
     1741 gattttgcaa acgcaataaa aaacggcaaa gtgtttgttg atggttggac tgccagccta
     1801 aacggaactt tacagagcta taaagaagat gtgaagattg tattttaaaa aaaatgaaca
     1861 taaaagccca cgcttcacag ccacacacat tgccaagtcg cacgaaaagt ccaacgcacg
     1921 accaaaactt gtcaaagtgt gtgtctgtcc gaacgcacaa ggtattgcaa aattgataac
     1981 taatttgatt gaaaatgaat aaagtccagc tgataaaatt gtatataaaa cattgggatt
     2041 taggtagtta tttaaacttt tgttctcgca tcaaagttag ttatattttg atagataact
     2101 gctccggaat ccccaacgtt tcatatactt agccgttagg cataattaat tataaaagac
     2161 acatggcaaa aaaaaacacc caacaacctg aactaataat tacaaaattg actattgaca
     2221 agtgtgaatt taaaaatgct ttaaatgaac gtattatttt aggcgaggaa atatataatc
     2281 ggacaattca aacaagttca gagtttaaga aaatgatgtc ggacttttca gcatggacag
     2341 actacaatac tgaatatctt aaacaatcct ttaataatcc tcataatgaa tataaaagag
     2401 cttatgaaga tgcaggacat tcattttttg tttcactagg tggcaataat aaaaatcctc
     2461 ttcagaaaaa aaaggaattc atatttgcta aaattgaaaa tcttaaaaag ttagttgcaa
     2521 aagccgacct acttaaaact tctttaacat cgacagttag ttcaactcct caacaagcca
     2581 aagttgcagt agcaatgaat gaagtcttta ttgttcacgg acaagacgag accgcaaaaa
     2641 ccaaaacagc tcgctttatt gaaaaattag gcttaaaacc aataatactt catgaacaag
     2701 caagtggaag caagactgta attgaaaaga ttgaagctta ttcaaatgta ggttttggaa
     2761 ttgtactata cacaccttgc gacatcggtg caaaaaatga aaaagaacct aattttaaaa
     2821 atcgcgcaag acaaaatgtt gtatttgaac acggtttttt aattggtaaa attggaagag
     2881 aaaatgtttg tgctttagtt aaaggtgaga ttgaaacacc aaatgacata tcaggcgttg
     2941 tgtatgtgga aatggatgat ggagaggctt ggcatgtcaa aattgcacga gaactaagaa
     3001 attcaggtta tgaaattgac atgaataaag taatttaata aaattgggaa aatttgactg
     3061 gacatatatt ggtacgcttt taagtgttac ctttggttgg ttcttaaatg agcttagtca
     3121 gtggtttaga acacgtaaag aagacaggaa aataaaaaag caagttttat tctatcttct
     3181 agaaacacat tttacattca ataaacttga tacaagtgaa ataacaaatc ttctttctca
     3241 aaaagtttta gaacgaattt ctaaaaacga acagcctgat gaagctaaac aatttattaa
     3301 ccaactctat gcggggatta ttagtggttt tgttgaagac aacgttgctg aatcactcga
     3361 agaaacagag gaacactaca caaaatcaat tagcgaactt tcaaaacttg acccaattac
     3421 agcctaccaa ttaaatggta aaaataaaat atttcaagtg tttgacttat tgcacgacta
     3481 ctatgataaa gtcaaagaaa ctcttccaaa tgaaccaggg ttaaatgaac aaattaaatt
     3541 gactattgaa acaatcaaac cagaaataat aaaagatgca atatctgatt tagaggacaa
     3601 aataagagat attgccctat caattaatct ttggactttg tatcgtgcaa gaagggcggt
     3661 gaacaacaca aagacaaaaa tacgcaagga cgatgaaaag aaaatagacg aattattgaa
     3721 tatggtaatg cccaaaatga cataataact gtgcataaca tgagttttgc gtcaggcggg
     3781 gtgaaggcaa attgaacctt tttcgctcta acgcaaagcc ccaaccacca gcagtacccc
     3841 tacaaagaaa tcgcaacaag gaaaaataaa gcaaatatat tgcacttgtt gtacttgttc
     3901 gggggttgtt gattttataa aatttggctt aacactatat caggattata ttgcaaccgt
     3961 aacaaaaaat tgtattggtt taacttgtat tatccgtaca atcgggcgga attttattaa
     4021 ttcatttctt gctttaaaaa agtgggctga aaacttctga aatcgtttct attactgctt
//
LOCUS       DAOOBH010000145         4009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_25131_length_4009_cov_7.058422, whole genome shotgun sequence.
ACCESSION   DAOOBH010000145 DAOOBH010000000
VERSION     DAOOBH010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25131_length_4009_cov_7.058422"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..485
                     /locus_tag="PK218_10770"
     CDS             120..485
                     /locus_tag="PK218_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99035.1"
                     /translation="MRRNVIRKIVGGLSLTSAMFVFQACYGTPQDFGLDLLIEGQVKS
                     KATGLPIKGIKVSVADNMQYELTDEEGMFSFYTEMLEGLKLQFQDIDSTQNGLYANKD
                     TVLTDLSKKMYLDIVLEEK"
     gene            486..1562
                     /locus_tag="PK218_10775"
     CDS             486..1562
                     /locus_tag="PK218_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HPW99036.1"
                     /translation="MFSSIKKYSFRKFRDSETKRHELNYLFWECTTRCNLNCLHCGSD
                     CSKDSSHQDMPAEDFFHAIDTIKEISQNFTVVFTGGEPLLRKDLELCGKELRKRGFKW
                     SIVTNGHLYDKQRHISLLNAGLGALTISLDGTNKSHNWLRNNEKSFDKVINAIELASS
                     SNRINFDIVTCVNQKNINELEQIKELLISKNVKAWRLFTIIPIGRASHNPDLTLTNNQ
                     FVQLMDFIVECRKSKEIDVKFSCEGYVGKYEPLVRDSYFFCRAGINIGSILIDGSISA
                     CPNIDRTFSQGNIYTDNFYSVWENKFQPFRDRKWTKLGQCESCKDFKDCQGNGFHNWH
                     GDKKNVLVCHNEKIEKTTKCQHRV"
     gene            1877..2083
                     /locus_tag="PK218_10780"
     CDS             1877..2083
                     /locus_tag="PK218_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW99037.1"
                     /translation="MNRIKEVLDEKGIKQTWLAEQLGKSYNMVNAYVQNRQQPRIEVL
                     YDIAKILGVNVKDLLTDNDIKKDN"
     gene            2083..>4009
                     /locus_tag="PK218_10785"
     CDS             2083..>4009
                     /locus_tag="PK218_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPW99038.1"
                     /translation="MSKEQKITTDIKVPNKDLEALRINFPHCFDKDGNFQLEKFKANL
                     TEKEINFSTESYGLDWLGKSYARLLASDPATTLLKEDETHNSKPENTNSENLLIKGDN
                     LEVLKHLVNAYYEQVKMIYIDPPYNTGSDGFVYEDDRKFTVRELQNLIGVDAEKAKRI
                     LDFTQQKSNSHSAWLTFMYPRLYIAKQLLKDEGIIIVSIDDNEVVQIRILLDEIFGED
                     NFVAQIIIDGTPKNDPYIVSTSHEYAVVYTKNKERAKNANYGNNDPIYAELISIFENN
                     NSDYEKTKIELDTFYKERNLKKDNISNYKYADEDGIFRIGPIDDPQNSGPKDERINPK
                     TGNPCKIPNRGWSCTVDTWNEWKEDNLIDFPNNDEVCPSKKTYIHKDRTDVMRSYFKI
                     QTRKDSTYLKTLFNTPFPPFANPKPVELLKQFILNTNDKNGIYLDFFAGSGSLGDAVN
                     QCNVLDNGERKFFLVQWPEPIIIKGNGKAKKIAKSAIDFIESKGISQPTIFEITKYRL
                     ILSSELLKNNNPLFTEKLGFKIFETTPIWEDYDFEAEQFDSTQTLFDIGKLSDDDIKA
                     LLTTWKTYDGIALTQELEKVDLNGYTAYYGIGKLYLMNKGFTTDHLKNLLEKIDSDKK
                     FNPTSIIAFGYHFESKSL"
BASE COUNT         1512 a          621 c          731 g         1145 t
ORIGIN      
        1 cacggtttga cagttgaatc gtccgcaatc cgccactagg catatgcgta acgttggcat
       61 tcattgtaag aaagcaacca gcaaacccta aaattcatca tagattaaaa atgattaata
      121 tgagaagaaa tgtgattaga aaaatagttg gaggattaag tttaacatca gcaatgtttg
      181 tttttcaagc gtgctatgga acacctcaag attttggact tgatttatta atcgaaggac
      241 aagttaaatc taaggcaacc ggattaccaa ttaaaggaat taaagtttcg gttgccgaca
      301 atatgcaata tgagctgact gatgaagaag gcatgttttc gttttacaca gaaatgcttg
      361 aaggtttaaa acttcaattt caagatattg attcaactca aaatggacta tatgctaaca
      421 aggatactgt attgacagat ttgagtaaaa aaatgtattt agatatagtt ttggaagaaa
      481 aataaatgtt ttcatcaatc aaaaaataca gtttcaggaa attcagagat tcagaaacaa
      541 aacggcacga actgaattat ttgttttggg aatgcactac aagatgtaat ttaaattgct
      601 tgcattgcgg aagtgattgc tcaaaagata gttcgcatca agatatgcct gcagaggatt
      661 tctttcatgc aattgataca ataaaagaaa tctcccaaaa ttttactgtt gtatttacgg
      721 gtggagaacc tttattacga aaggacttag aactttgtgg aaaagaactg cggaaacgag
      781 gttttaaatg gtcgatagtt acaaatggtc atttatacga caaacaaaga catatttcat
      841 tactcaacgc tggacttggg gcattaacaa taagtttaga cgggaccaat aaatctcata
      901 attggttgag aaataatgaa aagagcttcg ataaagtaat aaatgcaatt gaattggcat
      961 catcttctaa tcgtattaac ttcgatattg taacttgtgt aaatcaaaag aatatcaatg
     1021 aacttgaaca aataaaggaa ttgttaattt cgaaaaatgt caaagcctgg agattattta
     1081 caattattcc aatcgggaga gcctctcata atccagattt gacgttaaca aacaatcaat
     1141 ttgttcaatt aatggatttt attgttgagt gtcgtaaatc caaagaaatt gatgtgaaat
     1201 ttagttgtga aggttatgtt ggaaaatatg aaccattggt aagggattcg tatttctttt
     1261 gtagagcagg gattaatatt ggttcgattc ttattgatgg ttcgatttca gcttgtccaa
     1321 acattgatag gactttttct caaggaaata tttatacaga caatttttat tcggtttggg
     1381 aaaacaaatt tcaaccgttt cgagacagga aatggacaaa acttggtcaa tgtgaatctt
     1441 gtaaagattt taaagattgt caaggtaatg gatttcacaa ctggcatggt gataagaaaa
     1501 atgttttagt ctgtcataat gagaaaatag aaaaaacaac gaaatgccaa catcgtgtat
     1561 aaaacattgg ggtttaagtg gttattcgag cgttctgccc cgcatcggcg tttttgtcgg
     1621 cggataggta cgaagcccgc aatccccaac gtttcataca cgcgaccgtt atcggtcagt
     1681 gtaaaaagac agccaaccca caaatggcac atttggtttt gcccaacaca caagccgacc
     1741 cttcgcaaaa ccaaaagagc cattttttgc caacgcaccg caggacgact aaaaggaaat
     1801 tgattgcttt ttatcttgca aaatgagata caaataaaaa taaaatctat ctttgcgact
     1861 tgaatcattt gaacaaatga acagaataaa agaagtgctg gacgaaaaag gaattaagca
     1921 aacttggctt gccgaacaat tgggtaaaag ctacaatatg gttaatgctt atgtgcaaaa
     1981 cagacagcaa ccacgtattg aagtgcttta cgacatagct aaaattcttg gcgtgaacgt
     2041 gaaagaccta ttgactgaca acgatattaa aaaggacaac taatgagtaa agaacaaaaa
     2101 ataacgactg acataaaagt gccgaacaaa gatttggaag cattgagaat aaactttcct
     2161 cattgctttg acaaagacgg aaactttcaa cttgaaaagt ttaaagccaa tctgaccgaa
     2221 aaagaaatca acttttctac cgagagttac ggtttagact ggcttggaaa atcttatgca
     2281 agacttttgg caagcgaccc agcgaccact ttgctgaaag aagacgaaac tcacaacagc
     2341 aaaccagaaa acacaaattc agaaaacttg ctgataaaag gcgacaactt ggaagtatta
     2401 aaacaccttg tcaatgccta ctacgaacaa gtgaaaatga tttacataga cccgccttac
     2461 aacacaggaa gtgatgggtt tgtttatgaa gacgatagaa aattcacagt aagagaatta
     2521 caaaatttga ttggcgttga tgcagaaaaa gcaaaacgaa ttttagattt tacacaacaa
     2581 aaaagtaaca gtcattcggc ttggcttaca tttatgtatc cacgcttata cattgcaaaa
     2641 caattgctta aagatgaagg aataattata gtatcaattg atgataatga ggttgtgcaa
     2701 atcagaatat tattagatga aatattcggt gaagataatt ttgtagcaca aataattatt
     2761 gatggaacgc caaaaaatga cccttatata gtttcaacat cacacgaata cgcagtcgta
     2821 tacacgaaaa ataaagaaag ggctaaaaat gcaaattatg gaaacaatga cccaatttat
     2881 gccgaactaa tttcaatttt tgaaaacaat aatagtgatt acgaaaaaac aaaaattgaa
     2941 ttagacacat tttataaaga acgtaattta aaaaaagata atatttctaa ttacaagtat
     3001 gctgacgagg acggaatatt cagaattggt ccaattgatg accctcaaaa tagtggacct
     3061 aaagatgaaa ggataaatcc taaaacaggc aatccttgta aaatacctaa tagaggttgg
     3121 tcgtgtacgg tagatacttg gaatgaatgg aaagaagata atttaattga tttccctaat
     3181 aatgacgagg tttgcccgag caagaaaaca tatattcata aagacagaac tgatgtaatg
     3241 cggtcttatt ttaaaatcca aaccagaaag gactcaacat atctaaaaac actctttaac
     3301 acaccatttc caccttttgc aaatcctaaa cctgttgaac tattaaagca atttatttta
     3361 aatactaatg ataaaaatgg aatctatctt gatttttttg ctggttctgg tagtttggga
     3421 gatgccgtta atcaatgtaa tgtcttggat aatggtgaaa gaaaattttt tcttgttcaa
     3481 tggcctgagc caataataat caagggaaat ggaaaagcta aaaaaatagc aaaatctgca
     3541 attgatttta tcgaatcaaa gggtatctca caaccaacca tatttgaaat tactaagtat
     3601 cgattaattc tttcatccga actactaaaa aataataatc ccctttttac agaaaaactt
     3661 ggttttaaaa tatttgaaac gacaccaatt tgggaagact atgattttga agccgaacaa
     3721 tttgattcaa ctcaaacctt attcgacata ggaaaactct ccgatgatga tattaaagca
     3781 ttgctaacaa cttggaaaac gtatgatgga attgcgttga cacaagaatt agaaaaggtt
     3841 gatttaaacg gatacaccgc ttattatggc attgggaaat tgtatttgat gaacaaaggt
     3901 tttacgactg atcatctgaa aaatctttta gaaaaaattg attcagacaa gaaattcaat
     3961 cctacttcta tcattgcatt tggttatcac tttgaaagca aaagtttgc
//
LOCUS       DAOOBH010000146         3723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_27642_length_3723_cov_5.780534, whole genome shotgun sequence.
ACCESSION   DAOOBH010000146 DAOOBH010000000
VERSION     DAOOBH010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27642_length_3723_cov_5.780534"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(833..1255)
                     /locus_tag="PK218_10790"
     CDS             complement(833..1255)
                     /locus_tag="PK218_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPW99039.1"
                     /translation="MKRIIVDYAKLTGDILNLLVEKFPEGYDDSDIIRFRNAQNELIE
                     AVEVRTEDTIYLVKVSTKLASRMEKYDEDDDIDSVIEPIAPGKGIDLDDDDLSSDDED
                     DEVDLLKDKGGSDDDDDADDDDDFSDSDNFDEDDDGEE"
     gene            complement(1259..2233)
                     /locus_tag="PK218_10795"
     CDS             complement(1259..2233)
                     /locus_tag="PK218_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HPW99040.1"
                     /translation="MSKGPISQFIEKNYLHFNAAALVDAAKGYEEHLLDNGKMMITLA
                     GAMSTAELGKSLAEMIRQDKVHIISCTGANLEEDIMNLVAHNSYKRVPNYRDLSPQEE
                     WDLLENHYNRVTDTCIPEEEAFRRLQSHLFDIWNNADSKGERYFPHEFMYQMINSGVL
                     QQYYEIDPKDSWMVAAAEKNLPIVVPGWEDSTMGNIFASYCIKGTFKPTTMKSGIEYM
                     MWLADWYTKNCEGKGIGFFQIGGGIAGDFPICVVPMLYQDMEMHDVPFWSYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDITTPKFIVESDATIVAPLIFAYILDL"
     gene            complement(2329..>3723)
                     /locus_tag="PK218_10800"
     CDS             complement(2329..>3723)
                     /locus_tag="PK218_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HPW99041.1"
                     /translation="KYFDLINQTYYFPQEEFTLNKDSALQFHNIDLMKLVEQYGTPLK
                     FTYLPQISNNIKRAKNWFRNAMEKNKYEGKYFYCYCTKSSHFEYIMNEAFKNNIHIET
                     SSAFDINIVENLLENGKINKSTHIICNGFKRDQYITNIARLINNGHKNAIPIIDNYEE
                     LDLLQAEIKGKFKIGIRIAAEEEPKFEFYTSRLGIGYKNIVQFYKKQIQENDNLELKM
                     LHFFINTGINDTAYYWNELVKCIKVYIQLKKECPSLDGLNIGGGFPIKNSLAFDYDYQ
                     YMINEIINQIKIACDEAEVDVPNIFTEFGSFTVGESGGAIYQVLYQKQQNDREAWNMI
                     DSSFITTLPDTWAINKRFIMLAINRWNDTYERVLLGGMTCDSDDYYNSEQNMNAIYLP
                     KYNKEKPLYIGFFNTGAYQETIGGFGGLHHCLIPQPKHILIDRDENGILATEVFSEQQ
                     TADDVLKILGYTKK"
BASE COUNT         1126 a          655 c          588 g         1354 t
ORIGIN      
        1 agtgaacaca tttcatctaa aatgtaacac atttttaccc aaagtgaaca cattttaccc
       61 aaaatgtaac gcattttgac ccaaagtgaa cacattttat ctaaaatgta acacatttta
      121 cccaaaatgt aacacatttt tgggttttgg ctcaaatttt tgacctgaca gctctaattt
      181 ttgaccttaa atgttaaatt ttatataaat atttactttt ttataccaat tgatataata
      241 aacgcaatag gttttacgtt atatagttga aataggattt gagatttttc tataatttac
      301 taactaaata atttatacta tgagaatcag attttgaagg ttaaatttca ggaagttttc
      361 tgccacagcc tagatgcttt ttcgcagctg ttgctggcga aattattgta actattgctc
      421 cagggattaa ttttggtgtt gtaaattatg ggtgcatttg cgtacaagga aaacccttgg
      481 aagatgtaag ctttattaat gggcaattgg tgttaccaga aggtacagct acgattcggc
      541 atgatgtaaa caaatcgcgc cgaaaagtat ttagcgggtt gcacattact gaaacttact
      601 tcttttatgt ttatgttatc aattcagcga gtgtttcgcc tttgagcgac gttcgcaaat
      661 tgatggcggc ataaatttta attccgtttt ttttgtaaaa acccttttgg attgcgactg
      721 aagggttttt ttgtgctttg tcaaaaagta gttggctgtt ttgtttaaaa aaaaaccaaa
      781 tttcaattta ttcaattgga atttggaatt tttatattgg attttaggct atttattctt
      841 cgccgtcgtc atcttcgtca aagttatccg aatctgaaaa atcatcgtca tcatcagcgt
      901 catcgtcatc atcggaaccg cctttgtctt ttagtaaatc aacttcatcg tcttcatcat
      961 cacttgacaa atcatcatca tcaagatcaa ttccttttcc aggcgcaatt ggctcaatta
     1021 cagagtcaat atcatcatct tcatcgtatt tctccattct gctagccagt ttggtactca
     1081 cttttactaa ataaatagtg tcttcagttc gtacttctac tgcttcaatc aattcgtttt
     1141 gtgcatttct aaaacgaata atatcggagt cgtcatatcc ttccggaaat ttttctacta
     1201 gtaaatttaa gatgtcacca gtaagttttg catagtcaac gataattctt ttcataaatt
     1261 ataaatctag tatgtaagca aatattagtg gagcaacaat ggttgcatcc gattcaacaa
     1321 taaatttagg ggtagtaata tcaagttttc cccaagtgat tttctcattg ggtactgctc
     1381 cagaatagga gccataactt gttgtagagt ctgaaatttg acaaaagtag ctccaaaaag
     1441 gaacatcgtg catttccata tcttgataaa gcatcggtac tacacaaatt gggaaatcgc
     1501 cagcgattcc accaccaatt tggaagaaac caattccttt tccttcacaa tttttagtgt
     1561 accaatctgc caaccacatc atgtattcga tacctgattt catggtagtt ggtttgaacg
     1621 ttcctttaat acaataagaa gcaaaaatat ttcccattgt actatcttcc caacctggca
     1681 caacgattgg taaatttttc tcggcggcag caaccatcca agaatctttt ggatcgattt
     1741 cgtagtattg ttgcaacaca cccgaattaa tcatttgata catgaattca tgcggaaaat
     1801 aacgttctcc ttttgaatcg gcattattcc aaatatcaaa taaatgcgat tgtaatctac
     1861 ggaaagcttc ttcttcggga atacaagtat ccgttacacg attatagtgg ttttctaata
     1921 aatcccattc ttcttgaggc gataaatctc tatagtttgg cactctttta taggagttgt
     1981 gcgccaccaa gttcataata tcttcttcaa gattggcacc agtacacgaa ataatatgca
     2041 ctttgtcttg acgaatcatt tcggctaaag attttcctaa ttcggctgta ctcatagcac
     2101 cagccaatgt tatcatcatt ttaccattgt caagcaaatg ttcttcatag ccttttgcag
     2161 cgtcaactaa agcagctgca ttgaaatgaa ggtaattttt ttctatgaat tgactaattg
     2221 gtcctttgct cattttgttt tttattacga atagttcgtg cttcaaagaa aaactttttt
     2281 tctaaaacca caaacagtta ttactatttt attttgttaa tttttatact attttttagt
     2341 gtagcctaat atttttaata catcatccgc cgtttgttgc tctgaaaaaa cttcggtagc
     2401 caaaatgcca ttttcatctc tatcgattaa aatatgtttt ggttgcggaa tcaaacagtg
     2461 atgcaaacca ccaaatcctc caatagtttc ttgataagca ccagtgttga agaatccaat
     2521 atacaatggt ttttctttat tgtattttgg caaataaatg gcattcatgt tttgctcgga
     2581 attgtaataa tcatcgctat cacaagtcat tccgcccaat aaaactcttt cgtaagtatc
     2641 attccagcgg tttattgcca acatgataaa tcgtttgttg attgcccaag tatcgggtag
     2701 agtagtgatg aatgaactgt caatcatgtt ccaagcttca cggtcatttt gttgtttttg
     2761 gtacagaact tgataaatag caccacccga ttcgccaacg gtaaacgaac caaactcggt
     2821 gaaaatattt ggaacatcca cttcggcttc gtcacatgca atcttgattt gattgatgat
     2881 ttcattaatc atgtattggt aatcgtaatc aaaagctaaa gaatttttta ttggaaaacc
     2941 gccaccaata tttaatccgt ccaacgaagg acattctttt ttaagttgaa tataaacctt
     3001 aatacatttt accaattcat tccaataata tgcggtatcg ttgataccag tattaataaa
     3061 gaagtgaagc atttttaatt caagattgtc attttcttga atttgttttt tgtaaaattg
     3121 aacaatgttt ttgtatccaa tacctaagcg tgaagtataa aactcgaatt ttggttcttc
     3181 ttcggcagca attctaattc caattttgaa ttttccttta atttctgctt gaagcaaatc
     3241 taattcttca taattatcaa taattggaat agcgttcttg tgtccgttat tgattaatcg
     3301 tgcaatatta gtaatgtatt ggtctctttt aaaaccattg caaatgatgt gcgtgctttt
     3361 gttgattttg ccgttttcaa gcaaattttc tacaatatta atgtcaaacg cagaagaagt
     3421 ttcaatatga atgttgtttt taaaagcttc attcattatg tattcaaagt gtgagctttt
     3481 tgtgcaataa caatagaaat actttccttc atacttgttt ttttccatgg catttctaaa
     3541 ccaatttttg gctcttttaa tgttgttgga aatttgaggt aaataggtaa actttaatgg
     3601 tgttccatat tgctctacca atttcattaa atcaatgtta tgaaattgaa gcgcactgtc
     3661 tttgtttaaa gtaaattctt cttgagggaa atagtaagtt tgatttatta agtcgaaata
     3721 ttt
//
LOCUS       DAOOBH010000147         3681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_28045_length_3681_cov_6.020132, whole genome shotgun sequence.
ACCESSION   DAOOBH010000147 DAOOBH010000000
VERSION     DAOOBH010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28045_length_3681_cov_6.020132"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..965)
                     /locus_tag="PK218_10805"
     CDS             complement(171..965)
                     /locus_tag="PK218_10805"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPW99042.1"
                     /translation="MGQTPNETAHIFYYLTFLNNFDFIKNGLPDSSNLGVLWSIAIEE
                     QFYFIWPIILSFIPVKKMWIIFITIILGSITFRFLYQSPILYEHHTLSCIGDMAVGAL
                     GSWLVLQKSIFLEKIMNLKKAQIFLIYFVFILIFFFRKELFYWNEALKITERIIIAFI
                     ILLIILEQNYSTNSFFKMSKFKRISYLGTISYGLYCLHFIGILVTINATKILNINNHF
                     WQVMILETSIALFLTILISKLSFKFIEKPFLNLKNKFSFITKKASH"
     gene            complement(1609..>3681)
                     /locus_tag="PK218_10810"
     CDS             complement(1609..>3681)
                     /locus_tag="PK218_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPW99043.1"
                     /translation="SKTKIKVDVQGKKIEQEVHKVQILDPATGTGTFLAEVIKLIHKK
                     FEGQQGIWSNYVETHLLPRLNGFELLMASYAMAHLKLDLLLTETGYKPTINQRFKVYL
                     TNSLEEHHQDTGTLFANWLSTEANEANHIKRDTPVMCVIGNPPYAVSSTNKNDWIQDL
                     ISDYKKNLNERKINLDDDYIKFIRYGQHYIDKNGSGVLAYISNNSFIDGITHRQMRKH
                     LLETFDKIYILDLHGNAKKKEVCPDGSPDQNVFDIMQGVSINIFVKTSNKKKTELGEV
                     YHYDLQGKRNHKYETLNNSTISEIKWSKLEFKKPYYFFVPKDFESEEKYKNGFKVDEL
                     FIEKVPGIETIRDAITIHFKQDTLKNVVNDFLTLEENEIASKYNTKDARDWKISRAKE
                     DVKLNIDNKNVWQNIYYRPFDTRKTFYSGKQNGFVCNGRYNVMKHLLADNLALIVPRQ
                     TTQDYRHNFITNKIFEGNITASAKLFGTGNGFPLYIYPETNGQQTIEQTEERTPNLNA
                     EIVNQIAEELGLTFTNEKETTENTFSPIDILDYIYAVLHSPAYREKYKEFLKIDFPRV
                     PYPKDKNTFWQLVKLGGEIRQIHLLESSTVEKYITQYPIDGDNVVIKPNYQNGKVYIN
                     DTQYFDNVPETAWNFYIGGYQPAQKWLKDRKDRKLEFDDILHYQKIIVALTETDRLMK
                     EIDKIEIE"
BASE COUNT         1091 a          584 c          624 g         1382 t
ORIGIN      
        1 tgtcaagatg atttttttgg tttggtgtcg tcttttaagc tgccgcataa cggtcaggcg
       61 ctaaaagcag gccgaatctt tgcgttaaac tttatacgaa tatagaaatt aaaattcaat
      121 caaaaaactc aagtacgaag aatttatttc ggcttgcttt taacgacttg ttaatggctg
      181 gctttttttg ttataaatga gaatttattt ttcagattaa ggaatggttt ttcaataaat
      241 ttgaaactta atttactgat tagtatagtt agaaataagg cgatacttgt ttccaaaatc
      301 atgacttgcc agaaatgatt atttatgttc aatatttttg ttgcgtttat tgtgacaaga
      361 ataccaataa aatgaaggca atataatccg tacgaaatgg ttcctaaata gcttattctt
      421 ttgaatttag acattttaaa aaatgaatta gtggaataat tttgttctag aatgataagt
      481 aagataatga atgcgattat tattctttcg gttattttta aggcttcatt ccaataaaat
      541 agttcttttc taaaaaagaa gattagaatg aaaacaaaat aaataaggaa gatttgggct
      601 ttttttaggt tcattatttt ttccaaaaat atcgattttt gtaataccaa ccagctacca
      661 agagctccga cagccatatc accaatacaa gatagtgtgt ggtgttcata aagaatagga
      721 gattggtaga gaaatctaaa agtaatcgaa cctaaaataa ttgtaatgaa gataatccac
      781 atttttttga caggaataaa agataagatt attggccaaa taaaataaaa ttgttcttcg
      841 attgcaatac tccaaagtac acctaaatta gaagaatcgg gtagtccgtt tttaatgaaa
      901 tcaaaattat tcagaaaagt gagataatag aatatgtgag ctgtttcatt tggtgtttgc
      961 cccatgaaag atttgattag tgggaatatg aaaaaaccaa aaaaaaacgc ataaaaaata
     1021 tagtggccaa attcttagaa tccgcctcat ccaaaagaac ggaatattta ttttatgtcg
     1081 gatttccttt tctttaatca aaagataggt tattaaaaag ccacttagga cgaagaaaaa
     1141 attcacgcct aagtttccat ttccaaaaat tccctttttg attaatgtgt aatattcatc
     1201 gcttttaatt tcctgaaatt cagtatgaaa gctatgaaaa aagaaaactg ccaagaaaca
     1261 taagaatctt aatccatcta agttttcgaa aaatatttgc tgtggtttgt cctgtccgct
     1321 tgccattaac ggttgcgtgt atgaaacgtt tggcatttcg aagcgatttt ctatcaagtt
     1381 acaactactt ttgatacgag ctgaaacgct tgataaacca ctgactgcca aatgttttat
     1441 acacgttgtt gggtgctggt gttcattgtt tcctgtcatt tttcttacaa tttgctaatt
     1501 cgtttcggtc tgccgtgcgt tgggacagac acactctttg acaagctttg gttttagcgt
     1561 tggctcgtgg ggcttggcaa tgtgtgtggc tgtgaagcga gggctttttc attctatttc
     1621 aattttatca atttctttca ttaatctgtc tgtttcagtc aatgcaacaa ttattttttg
     1681 ataatgcaaa atatcatcaa attcaagttt gcggtcttta cggtctttga gccatttttg
     1741 ggcaggttgg tagccgccaa tgtaaaagtt ccatgccgtt tcgggtacat tatcaaaata
     1801 ctgcgtgtca ttgatgtaaa cctttccgtt ttgataattt ggtttgatta ccacattatc
     1861 gccatcaatt gggtattgtg ttatatattt ctcaactgtt gagctttcta ataaatgtat
     1921 ttgacgaatt tctccgccca gttttaccaa ttgccaaaac gtatttttgt cttttgggta
     1981 cggcactctt gggaagtcta tttttaaaaa ttctttgtat ttttctctgt aagcaggcga
     2041 atgcaaaacc gcatagatgt aatctaaaat gtcaattggg gaaaaggtgt tttccgtagt
     2101 ttccttttcg ttggtaaacg ttaaccctaa ttcttccgca atttggttta caatttcagc
     2161 gtttagattt ggtgttcttt cttctgtttg ttcaattgtt tgttgtccgt tggtttctgg
     2221 atagatataa agcggaaaac catttcctgt accgaacaat ttggcacttg ccgtaatatt
     2281 tccttcaaaa atcttattag ttataaagtt atgtctgtaa tcttgtgttg tttgtctagg
     2341 aactatcaaa gctaaattgt ctgccaataa atgtttcata acattatatc taccattaca
     2401 aacaaacccg ttttgtttcc ctgaatagaa agtttttcta gtgtcgaatg gtctgtaata
     2461 aatattttgc caaacatttt tattgtcaat atttagtttt acatcttctt ttgctctgct
     2521 aattttccaa tctctcgcgt ctttagtgtt atattttgat gcaatttcat tttcttccaa
     2581 agtgagaaaa tcatttacta cattttttaa agtgtcttgt ttaaaatgta tggtaattgc
     2641 gtcacgaatt gtttctatac ctggcacttt ttcaataaat aattcatcca ctttaaatcc
     2701 attcttatac ttctcttcag attcaaaatc tttcggaaca aaaaagtaat aaggtttttt
     2761 gaattcaagt tttgaccatt ttatttcaga aatagttgag ttattaagag tttcatattt
     2821 gtggttcctt ttaccctgca aatcataatg ataaacttct cctaattccg ttttcttttt
     2881 gttgcttgtt tttacaaaaa tatttatcga aacaccttgc ataatatcaa aaacattttg
     2941 gtcagggctt ccatcagggc aaacttcttt tttcttggca tttccgtgta aatccaagat
     3001 gtaaatttta tcaaaagtct ctaaaaggtg tttacgcatt tgacggtgtg taattccgtc
     3061 aataaaactg ttattagaaa tgtaagccaa aacgccactt ccatttttgt cgatataatg
     3121 ttgtccgtag cgaatgaatt tgatgtaatc atcatccaaa ttgattttac gttcattgag
     3181 atttttcttg tagtcagaaa ttaaatcttg aatccaatca tttttattgg tgctacttac
     3241 cgcataaggt ggatttccaa tcacacacat tactggcgta tctcgtttta tgtggttggc
     3301 ttcattggct tcggtgctta accaattggc aaataaagtt cctgtgtctt ggtggtgttc
     3361 ttctaaactg ttggtaaggt aaactttgaa tctttggttt attgttggtt tgtagcctgt
     3421 ttcggttaaa agcaaatcta attttaaatg agccatagca taacttgcca ttagcaactc
     3481 aaaaccattg aggcgtggca ataaatgggt ttctacataa ttactccaaa tgccttgctg
     3541 tccttcaaat tttttgtgga tgagtttaat aacttctgct aaaaatgttc cagtgcctgt
     3601 ggctgggtca agtatttgaa ctttgtggac ttcctgttct atcttttttc cttgtacgtc
     3661 aacttttatt ttggttttgc t
//
LOCUS       DAOOBH010000148         3630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_28565_length_3630_cov_6.446154, whole genome shotgun sequence.
ACCESSION   DAOOBH010000148 DAOOBH010000000
VERSION     DAOOBH010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28565_length_3630_cov_6.446154"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PK218_10815"
     CDS             complement(<1..413)
                     /locus_tag="PK218_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99044.1"
                     /translation="MEKRITETELILPSLYLMSLNNGVISTSELIQKLRDIMKPTGED
                     LKILAGRSDDKFSQKVRNLIAHRTFERFGYAEYDAGQIGITKEGKAHLENNQDILKYL
                     LINDFSYTDLTESLRKVEKNQNKKKIETFDENIII"
     gene            complement(417..1616)
                     /locus_tag="PK218_10820"
     CDS             complement(417..1616)
                     /locus_tag="PK218_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HPW99045.1"
                     /translation="MKNSVKEPIATYNQIGLDFGNYVESRKLYPILKWAGGKEQELKY
                     IVPNLPEKFKNYYEPFVGGGAVYTALQADKYFVNDKSEELISLYRSIINSDRTLFFKA
                     LDEIIHNWDLLTLVVKKNDVFFVETYKKYSSNKIEETKLKDILFEFILKHADQFNGMF
                     SSMFNFNIENFIKEIKINLIRKIKRMKQLEKEKYLLPDNDILDNIETALKSAFYMHFR
                     HIYNNTEKYKINSAFKSAIFLFIRNFAYSGMFRYNSKGDFNVPYGGIAYNNKNFSKKV
                     EYLKTKELKSLLDNTVIENLDFEDFFKQHTPENNDFVFLDPPYDSEFSTYAKNEFTKK
                     DQIRLANYLIKKCKGKWMMIIKNTDFIFELYKNKGLNIQSFDKKYLVSFMNRNDKNVE
                     HLLITNY"
     gene            complement(1725..2177)
                     /locus_tag="PK218_10825"
     CDS             complement(1725..2177)
                     /locus_tag="PK218_10825"
                     /inference="COORDINATES: protein motif:HMM:NF036614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HPW99046.1"
                     /translation="MENFENIQSVNQTLTDDSKIKTYLLEISKWGKFLAIVGYIGIAV
                     LAIVAIALIVISFITSIKLDVGLSLGTFGVIYFALAILYYFPVKFLYKFSIQIKKGLE
                     SDNNQDITLGFENLKSLFKFTGILTIVILSIYALLLVIGLPIILLIAN"
     gene            complement(2208..2369)
                     /locus_tag="PK218_10830"
     CDS             complement(2208..2369)
                     /locus_tag="PK218_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99047.1"
                     /translation="MNKRLNRELQIANLLAERQKLIELMQKLKQKTYELAQQEAEAKA
                     ETENNAKID"
     gene            complement(2543..3517)
                     /locus_tag="PK218_10835"
     CDS             complement(2543..3517)
                     /locus_tag="PK218_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HPW99048.1"
                     /translation="MKKILGIGNALVDILMILRSDRALDKLQLPKGSMQLIDETTLEK
                     IQDETLLLNKHIATGGSAANTIISLARLGVNVGFIGKIGNDSVGEFFNGDLFENGVLT
                     FLSYSMLPSGRCHVLISPDSERTMCTYLGAAANLQANDLKINMFKGFDILHIEGYLVN
                     DQNLLLAAARLAKRAKMKISIDLASYNVVEDNLSFLTDFVKQYVDIVFANEKEAFVFT
                     KQRNEKAAEKIGNFCEIAIVKVGRRGSYIYSKGHCTHIPSVKVNSIDTTGAGDLYAAG
                     FLYGLSHELPLDQCGKIGTICAGHVIRCVGAKMDDRTWKIINETVNTF"
BASE COUNT         1105 a          591 c          589 g         1345 t
ORIGIN      
        1 tgtataatta tattttcatc aaatgtttca atcttttttt tgttttgatt tttttcgact
       61 tttctaagac tttctgttaa atctgtatat gaaaaatcat tgattaataa atatttcaaa
      121 atatcttgat tgttttctaa atgtgcttta ccttcttttg tgattcctat ttgtccggca
      181 tcatattcag cgtagccaaa tcgctcaaaa gttctatgtg caattaaatt tctaactttt
      241 tgagaaaatt tatcatcact tcttccggct aaaattttta aatcttcccc tgttggtttc
      301 attatatctc taagtttttg aattaattca cttgttgaaa ttaccccgtt gtttaaactc
      361 atcaaatata aagaaggaag aatcaactcg gtttctgtaa ttctcttttc catattttaa
      421 taattagtga ttaataaatg ctctacattt ttgtcattcc tattcataaa actaacaaga
      481 tattttttgt caaaagattg aatatttagt cctttatttt tataaagctc aaaaatgaaa
      541 tctgtgtttt ttattatcat catccatttt cccttacatt tttttattaa ataatttgca
      601 agtctgattt ggtctttttt tgtaaattca tttttagcat aggtgctaaa ttcactatca
      661 taaggagggt ctaaaaaaac aaagtcattg ttttcaggtg tgtgttgctt aaaaaaatct
      721 tcaaagtcta aattttctat cacagtattg tcaagcaatg attttaattc tttagttttt
      781 aaatattcaa cttttttaga aaagttttta ttgttgtatg caatgccacc gtaaggaaca
      841 ttaaaatctc cttttgaatt ataacgaaac ataccactgt atgcaaaatt ccttataaat
      901 aaaaaaatgg cagatttaaa agcactatta attttgtatt tttcagtatt gttgtaaata
      961 tggcgaaaat gcatataaaa tgcactttta agagctgttt ctatattgtc taaaatatca
     1021 ttatcaggta aaagatattt ttctttttcc aattgtttca ttctcttaat ctttcgaata
     1081 agattgattt ttatttcttt aatgaagttt tcaatattaa aattaaacat tgatgagaac
     1141 attccattga attggtctgc atgttttaat ataaactcaa ataaaatgtc cttaagtttc
     1201 gtttcttcaa ttttgttact tgaatatttt ttgtaagttt caacaaaaaa aacatcattt
     1261 ttctttacaa ccaaagttaa taaatcccaa ttgtgtatta tttcatctaa tgctttaaaa
     1321 aataaagttc tatcactatt tattatgcta cgatataaag aaataagttc ttcggattta
     1381 tcattaacaa aatatttatc agcttgtagt gctgtataaa cagcaccacc accgacaaat
     1441 ggctcataat aatttttgaa tttttcaggc agattgggta ctatgtattt taattcttgt
     1501 tctttaccac cagcccattt taaaataggg tataattttc tagattcaac ataattacca
     1561 aaatcaagtc caatttggtt atatgttgca ataggttctt ttactgaatt tttcatacta
     1621 caaatttaat caaattttcg ataataataa attttagcat gaatggtaac gaatttgact
     1681 taattagttt tggtgttttc gctggcgtgg ttttcccgtt tttattaatt cgcaattaat
     1741 aagattatcg gtaatccaat aaccaaaagt agagcataaa ttgataaaat tactattgtt
     1801 aaaattcctg taaacttaaa cagcgatttt aagttttcaa atcctaaagt aatgtcttgg
     1861 ttattatccg attctaagcc ttttttaatt tgaattgaga atttgtacag aaattttacc
     1921 ggaaaatagt ataaaattgc gagggcaaag tagattacgc caaatgttcc caaagacaag
     1981 cctacatcta atttaatact ggtaatgaaa gatataacta ttaaagcaat ggcgacaata
     2041 gccaaaactg caattcctat atatccgaca atagccaaga attttcccca tttggagatt
     2101 tctaaaagat acgttttaat tttactgtca tcggttaggg tttgatttac cgattggatg
     2161 ttttcaaagt tttccatgtg gtttgttttt agtggttaat atttaattta atcaattttg
     2221 gcgttgtttt cggtttcagc ttttgcctct gcctcttgct gtgccagttc gtaggttttc
     2281 tgtttcagct tttgcattaa ttcaataagt ttttgccttt ctgctaatag atttgctatt
     2341 tgcaactctc tgtttaatcg tttgttcatg gtaattaatg tttaaaaatt aagtagaata
     2401 aagaactttg gacaaaggta caaattattt acacaattgt tacaaaatat tggttacatt
     2461 tttgcccgaa aatgcaaaaa aacagaattg caagaagtgc aaagtgcagg agtaaatttg
     2521 tgtgcgtcgt agaagcttaa ttctaaaaag tattgacggt ttcgttgata attttccacg
     2581 tacggtcgtc catttttgca cccacgcagc gaataacgtg tccggcgcag atggtgccta
     2641 ttttaccgca ttggtcaagc ggtagttcgt gcgagaggcc atagagaaat ccggcagcgt
     2701 aaaggtcgcc tgcgccggta gtgtcgatgc tgtttacttt cacagacgga atgtgtgtgc
     2761 aatgcccttt tgaatagatg tacgacccgc gccgtcctac ttttactatg gcaatttcgc
     2821 aaaaattgcc aattttttca gcggcttttt cgttgcgttg tttggtgaag acgaacgctt
     2881 ctttttcgtt ggcaaaaaca atatcaacgt attgttttac aaaatcggtg agaaacgaca
     2941 gattgtcttc taccacatta tagctggcaa ggtcgataga tattttcatt ttcgcccgtt
     3001 ttgctaatct tgctgcggca agcagtaggt tttggtcatt gacaaggtag ccttcgatgt
     3061 gtaaaatatc aaaaccttta aacatgttaa ttttaaggtc gttagcttgc aaattggcag
     3121 ccgctccgag ataagtacac atagtgcgtt ccgagtccgg cgaaatgagc acgtggcagc
     3181 gtcccgaagg taacatcgag taggagagaa aggtgagtac accattttca aataaatcgc
     3241 cattgaaaaa ctcgcccacg gaatcgttgc ctattttgcc tatgaagccg acgtttacgc
     3301 ccaaacgtgc caaactgata atggtgttgg ctgccgaacc gccagtagca atgtgtttgt
     3361 tgagaagtag ggtttcgtct tgaatttttt ccagagtagt ttcatctatt aactgcatac
     3421 ttcctttggg aagttggagt ttgtccaatg ctctatcgga tcggagaatc attaaaatat
     3481 cgacgagagc gttgcctatg cctaatattt ttttcatacc gttggcgata aaaagaaaaa
     3541 aagaagccgc ttctcaatac ataagttgcg gctgtttttt gctctccctc ttggacttga
     3601 accaaggacc ctctgattaa cagtcagatg
//
LOCUS       DAOOBH010000149         3620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_28673_length_3620_cov_5.858626, whole genome shotgun sequence.
ACCESSION   DAOOBH010000149 DAOOBH010000000
VERSION     DAOOBH010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28673_length_3620_cov_5.858626"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..509)
                     /gene="rplT"
                     /locus_tag="PK218_10840"
     CDS             complement(165..509)
                     /gene="rplT"
                     /locus_tag="PK218_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPW99049.1"
                     /translation="MPRSVNSVAKRARRKKIMKQAKGFFGRRKNVWTVAKNAVEKAMV
                     YAYRDRKQNKRNFRALWIVRINAGARLHGMSYSQFMGLVKKNGIELNRKVLADLAMNH
                     PEAFKAIVNKVK"
     gene            complement(610..807)
                     /gene="rpmI"
                     /locus_tag="PK218_10845"
     CDS             complement(610..807)
                     /gene="rpmI"
                     /locus_tag="PK218_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPW99050.1"
                     /translation="MPKMKTKSSAKKRFKVTGSGKIKRKHAFKSHILTKKSKKRKLAL
                     THSALVHKTDEKSIKQQLRII"
     gene            complement(911..1408)
                     /gene="infC"
                     /locus_tag="PK218_10850"
     CDS             complement(911..1408)
                     /gene="infC"
                     /locus_tag="PK218_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPW99051.1"
                     /translation="MNKLIRATEVRLVGENVEPGIFKIADALRMAEELEVDLVEISPN
                     AEPPVCKLMDYGKFLYQQKKREKELKAKSTQITVKEIRFGPQTDEHDYEFKRKNAEKF
                     LKEGSKLKAFVFFKGRSIIYKEQGQILLLRLATDLEEYGKVEAMPVLEGKRMIMFIAP
                     KKKSK"
     gene            complement(1485..3431)
                     /gene="thrS"
                     /locus_tag="PK218_10855"
     CDS             complement(1485..3431)
                     /gene="thrS"
                     /locus_tag="PK218_10855"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPW99052.1"
                     /translation="MIKITLPDGSVKDFENGITSMDVAKSISEGLARNVISASFNGVA
                     IETSTELNTNGALTLYTWNDKDGKKAFWHSTSHVMAQVLEEFYPGIKLTLGPAIDNGF
                     YYDVDFGDQKITDADFKKIEDRVLEISREKHEFRMRSVSKEEALEIYKDNEYKTELIS
                     NLEDGTITFCDHANFFDLCRGGHIPNTGIIKAMKIMSVAGAYWRGDEKNKQLTRVYGI
                     SFPKQKDLTEYLELLEEAKRRDHRKLGKELDLFTFSQKVGQGLPLWLPKGAALRDRLE
                     QFLKKAQKKAGYEQVVTPHIGQKELYVTSGHYAKYGADSFQPIHTPAEGEEFLLKPMN
                     CPHHCEIYNTKPWSYKDLPKRYAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCTPD
                     QLDSEFKKVIDLVLYVFGSLGFENFTAQISLRDQENREKYIGSDENWEKAENAIINAA
                     ADKGLNTVVEYGEAAFYGPKLDFMVKDALGRSWQLGTIQVDYNLPERFELTYKGADDK
                     LHRPVMIHRAPFGSMERFIAILLEHTVGNFPLWLMPEQAIILSLSEKYENYAKKVLEL
                     LENHEIRALIDNRNETIGKKIRDAEMQKLPFMLIVGEEEEKNGTISVRRHGQEGKGNS
                     TVTIEEFVSIVNVEISKTLKSFEV"
BASE COUNT         1098 a          673 c          488 g         1361 t
ORIGIN      
        1 ttagtaaagg tattaatttt aaatggataa tttaacattt cgttttcttt tgttaaagaa
       61 agttaataca aaatgaagaa atattttagg tataacaaaa aaaatcccaa tctttcgatt
      121 aggatttctt aatataagta agttaaataa ggttgtacaa acgtttattt tactttattc
      181 acaatagctt tgaaagcttc tggatgattc attgctaaat cagctaatac tttacggttc
      241 aattcgatac cgtttttctt aactaatccc atgaattgag aatatgacat tccgtgtaat
      301 ctagcaccag cgttaatacg cacaatccat aaagcacgga agtttctttt attctgtttt
      361 ctatcgcgat aagcataaac catcgctttt tcaactgcgt ttttagcaac tgtccaaacg
      421 tttttacgac gaccaaagaa acctttggct tgcttcatta tcttttttct tcttgctctt
      481 ttagcaactg aatttactga tcttggcata atttaattgt tttttgtagc aggcgaaccg
      541 aatttaatca aagttactta aagccatact ccaaggttat ttaaaattga tttttaacct
      601 aaagaattat tagataattc ttaattgttg tttaatactt ttctcatctg ttttgtgtac
      661 caaagcagag tgtgtcaaag ctagtttacg cttcttagac tttttagtca agatgtggct
      721 tttgaaagcg tgttttcttt taatttttcc agaaccagta actttaaatc gtttcttggc
      781 actagatttt gttttcattt taggcataat ttccctaatt tatttaaatt taacttactt
      841 acttttattt tgattttgaa aaaatcaatc tcattatttt tattttccac tgctttttgc
      901 aaacggaata ttatttactt tttttcttcg gagcaatgaa cataatcatt cttttacctt
      961 ccaaaacagg cattgcttca acttttccat attcttccaa gtcagtagct aaacgcaaca
     1021 ataagatttg accttgctct ttataaataa tcgaacgacc tttaaagaat acaaaagctt
     1081 ttaactttga accttcttta aggaattttt cggcattctt tcttttaaac tcataatcat
     1141 gctcatccgt ttgaggacca aaacgaattt ccttaactgt aatttgagta gattttgctt
     1201 ttaattcttt ttcacgtttc ttttgttggt atagaaattt tccataatcc attaatttac
     1261 aaactggtgg ttcagcattt ggagaaatct caaccaaatc tacttcaagt tcttcagcca
     1321 ttcgtaaagc atcggctatt ttgaaaatcc ctggttctac attttctcca actaaacgaa
     1381 cttctgtagc acgtatcaac ttattgatgt tatgtgctgc tgttttttct actcgaggtt
     1441 tgtagcctct attgtttctt attgctatgg cttaaaaatt taaattatac ttcaaatgat
     1501 tttaatgtct tacttatttc tacgttaact attgaaacaa attcttcaat tgtaacggta
     1561 ctatttcctt ttccttcttg accatgacga cgaacagaaa ttgttccatt tttttcttct
     1621 tcctcgccaa caatcagcat aaatggcaat ttttgcattt ccgcatctct aatttttttg
     1681 ccaattgtct cgttcctgtt gtcaattagg gcgcgaattt cgtgattttc tagcaattct
     1741 aaaacttttt tcgcataatt ttcatatttt tcactcagtg acaagattat agcttgttca
     1801 ggcatcagcc acagtgggaa atttcctacc gtatgttcta gtaatattgc aataaaacgc
     1861 tccattgagc caaatggagc acgatgaatc attactggtc gatgtaattt gtcatctgct
     1921 cctttataag ttaattcaaa acgttcgggt aaattataat ccacctgaat agttccaagt
     1981 tgccaacttc tacctaaagc atctttaacc ataaaatcta attttggacc ataaaaagca
     2041 gcttcaccat actcaacaac ggtatttaaa cctttatctg cggctgcatt gataatggcg
     2101 ttttcggctt tctcccaatt ttcatcgcta ccgatatatt tttcacgatt ttcttgatct
     2161 cgaagtgaaa tttgagcagt aaaattctca aatcctaaag aaccaaaaac atacaaaact
     2221 aaatcaataa cttttttaaa ttcagaatct aattgatcag gtgtacaaaa tatatgggca
     2281 tcatcttgag taaaacctct aacacgagtt aaaccatgta attcaccact ttgttcatag
     2341 cgatacaccg ttccaaattc agcataacgt tttggtaaat ctttatatga ccaaggtttt
     2401 gtattgtaaa tttcacaatg atgtggacaa ttcatcggtt ttaataaaaa ctcttctcct
     2461 tctgcaggtg tatgaatagg ctggaaacta tcagcaccat atttagcata atgtcccgag
     2521 gtaacataca attctttctg acctatatgt ggtgtaacta cttgctcgta tcctgctttc
     2581 ttttgtgctt tttttaggaa ttgttcaagt ctatctctta aagcagctcc ttttggtaac
     2641 cataatggta atccttgacc aactttttga gaaaaagtaa ataaatccaa ttctttacct
     2701 agttttctat ggtctcttct ttttgcttct tctaataact ctaaatattc agttaagtct
     2761 ttttgttttg gaaatgaaat tccataaaca cgtgttaact gcttattctt ttcatcgcca
     2821 cgccaataag ctccagcaac actcatgatc ttcatggctt tgataattcc agtattcgga
     2881 atatgtccac cgcgacacaa gtcaaagaaa tttgcatgat cacaaaaagt aatggtgccg
     2941 tcttctaaat tcgaaatcaa ttcagttttg tattcattgt ctttatatat ctctagagct
     3001 tcctccttag aaacagatcg cattctaaat tcatgtttct ctcttgatat ttctaacact
     3061 ctatcttcta tttttttgaa atcagcatct gtaatttttt gatcaccaaa atcaacatca
     3121 tagtaaaaac cattatcaat cgcagggcca agtgttaatt taataccagg ataaaattcc
     3181 tcaagcactt gtgccataac gtgtgatgta gaatgccaaa atgctttctt gccatctttg
     3241 tcattccaag tgtataatgt taacgcacca ttagtattta actccgttga tgtttcaatt
     3301 gcgacaccat taaaagaagc tgaaataacg tttctcgcca atccttcact aatgctttta
     3361 gcaacatcca tagaagtgat gccgttttca aaatctttaa ccgagccatc tggcaatgta
     3421 atttttatca ttcctaaata tattatggga tgcaaatata gacgattaac aaaatagata
     3481 caatatctgt ttctatattt ttaataataa taaaaaaggc ataattatta cttagttttt
     3541 ttttagcgat tttatctatg tctttctttt taaaatatta taacaataaa ttcaagaata
     3601 aagtacgcac aatgagggca
//
LOCUS       DAOOBH010000150         3521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_29660_length_3521_cov_6.599827, whole genome shotgun sequence.
ACCESSION   DAOOBH010000150 DAOOBH010000000
VERSION     DAOOBH010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29660_length_3521_cov_6.599827"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PK218_10860"
     CDS             <1..225
                     /locus_tag="PK218_10860"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPW99053.1"
                     /translation="PVTIKFMGKETVSAGNLGKKECYKISIGAKTDALRGKDKNLIWL
                     TADAKKIPALLEFSIPVGTGKLVLTSATGI"
     gene            228..554
                     /locus_tag="PK218_10865"
     CDS             228..554
                     /locus_tag="PK218_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99054.1"
                     /translation="MRKLFLILAILFLVLSILFTLLPMGTIAFLPIGLALISSLVLLK
                     KSAENQRRLPNWLLTLSAICSFVVVLKLVFIKDKVEVDKKFEQQKIETKIEAKKELEE
                     LENDLE"
     gene            646..2019
                     /locus_tag="PK218_10870"
     CDS             646..2019
                     /locus_tag="PK218_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPW99055.1"
                     /translation="MRNILIIGAGRSASSLIKYLLNKSQEENLQITIADLSLELAQKK
                     TNNHPNAKAIALDINNIEQRQTEVQKADIVISMLPAHMHIEVAKDCVIFKKNMVTASY
                     ISDAMQSLDAEVKANGLIFMNEIGLDPGVDHMSAMKVIDEIRAKGGKMLLFESFCGGL
                     VAPESDTNLWNYKFTWAPRNVVLAGQGGAAKFIQEGKYKYIPYQKLFRRTEFLHVEGY
                     GRFEGYANRDSLKYRTVYGLDDVLTLYRGTIRRVGFSKAWNMFVQLGMTDDTYIIDDS
                     EHISYRDFINLFLPYSPTDSVEIKTRLALGIEQDDIMWDKLLELDLFNPNKIVGLKDA
                     TPAQILEHILNASWTLQPEDKDMIVMYHKFGYELNGKQEQIDSKMVCIGDDQTYTAMA
                     KTVGLPVAIAALQILNGKIKTPGVQLPITKEVYEPILKELETFGVIFNETKMPYIGYN
                     PDRLIGN"
     gene            2122..2592
                     /locus_tag="PK218_10875"
     CDS             2122..2592
                     /locus_tag="PK218_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HPW99056.1"
                     /translation="MKINQLQIEIDGIDKEILRDLMEDARKPILQIANKIGISGAAIH
                     QRLRKLEDAGVIAGSKFIIDYKVLGYKTMAFIGVYLDKASSNSDAVRELKKIPEVLEC
                     HYTTGNWSILVKIICKDNEHLMQLLNKKIQPIDGVSRTETFISLEQQIERQIQI"
     gene            complement(2690..3385)
                     /locus_tag="PK218_10880"
     CDS             complement(2690..3385)
                     /locus_tag="PK218_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPW99057.1"
                     /translation="MIGYYILLGLIALVSWAISSKLKSKFEEYSKVHLRNGMSGAEIA
                     EKMLADNGIYDVRVISTPGRLTDHYNPADKTVNLSEAVYNQRNAAAAAVAAHECGHAV
                     QHATAYAMLTMRSKLVPLVNVSSTLSQWLIFGGLILGAAAGLGFGFYVAVIGLVLMAV
                     TTAFSFITLPVEYDASNRALAWLKNKNMLSQQEYAGAEDALKWAARTYVVAAIGALAS
                     LLYWAFQVFGRRD"
BASE COUNT         1164 a          655 c          662 g         1040 t
ORIGIN      
        1 ccggtgacta taaaatttat gggtaaagaa acggttagcg caggaaatct tggcaaaaaa
       61 gaatgctaca aaatttctat tggtgctaaa actgatgcct tgcgcggaaa agataaaaac
      121 ctaatttggc tgactgcaga cgctaaaaag attcctgctt tattagagtt cagcattcct
      181 gttggaaccg ggaaattggt attaacctca gcaactggta tataattatg agaaaacttt
      241 ttttaatctt agccatttta tttttagtat tatctatctt gtttacacta cttcccatgg
      301 gaactattgc atttcttcct attggtttag ccttaatttc gagtttagtc cttttaaaaa
      361 aatcagccga aaaccaacgc agattaccca attggttgct aacattaagt gctatttgtt
      421 cttttgtggt agtgctaaaa ttagtgttta taaaagacaa agtggaagta gataaaaagt
      481 ttgaacaaca aaagatagaa actaaaatag aagccaaaaa agaattggaa gaactagaaa
      541 acgacttaga ataaacacac aaagcacttc agattttcgg agtgcttttt tgtttctatc
      601 atttaatccc aatttcatac atttgccaca acaacaaatt aaagcatgag aaacatactt
      661 atcattggtg ctggtcgctc ggcatcatct cttataaaat atcttttaaa taaatctcaa
      721 gaggaaaatc ttcaaattac cattgccgat ttatcgttag aattggctca aaaaaagacc
      781 aacaatcatc ctaatgcaaa agccatcgca ttagacatca ataatattga acaacgtcaa
      841 actgaagttc aaaaagcaga cattgtcatc tcaatgttgc cggcgcacat gcatattgaa
      901 gtggcgaaag attgtgtgat tttcaagaaa aacatggtga cggcttccta tatttcagac
      961 gctatgcaaa gtttggatgc cgaagtaaaa gccaatgggt taatcttcat gaatgaaata
     1021 ggtcttgacc caggtgttga tcacatgagt gccatgaaag taattgacga gattagagct
     1081 aaaggcggaa agatgctgct tttcgaatct ttttgtggcg gattagtagc tcccgaaagt
     1141 gatacgaatc tttggaacta caagtttact tgggcaccaa gaaatgtagt attggcaggt
     1201 caaggtggtg cagcaaagtt tattcaagaa ggaaaataca aatacattcc ctatcaaaaa
     1261 ttattccgta gaaccgagtt ccttcatgtg gaaggttatg gcagatttga aggttatgcc
     1321 aatcgtgatt cgttaaaata cagaacggtt tatggattgg atgatgtgct tactttatac
     1381 cgtggcacca ttcgtagagt tggattctct aaagcctgga acatgtttgt gcaacttggc
     1441 atgaccgacg acacttatat tatcgacgat tcggaacaca tcagttaccg tgattttatc
     1501 aatttatttt tgccctattc gccaacagat tcggtggaaa ttaaaacccg tttggctctt
     1561 ggcatagagc aagacgatat tatgtgggac aaactcttgg aactcgattt attcaaccca
     1621 aataaaattg ttggtttaaa ggatgctact ccagctcaaa ttcttgaaca tattttgaac
     1681 gctagttgga ccttgcaacc cgaagacaaa gacatgattg taatgtatca caagtttggc
     1741 tacgaactaa atggcaaaca agaacaaata gactctaaaa tggtgtgtat tggcgatgac
     1801 caaacctata ccgccatggc aaaaaccgta ggcttacctg tggcaattgc agctcttcaa
     1861 atattgaatg gaaaaattaa aaccccaggc gtgcaattgc caattaccaa agaagtttat
     1921 gaaccgattc ttaaagaatt agaaactttc ggcgtaattt ttaatgaaac caaaatgcct
     1981 tatataggat acaatccaga tagattgatt ggaaattaac tcaatgtctg ctacaacact
     2041 caaaccaaaa tcgtaactga ttttggtttt ttttgtgcaa tttgttactt attccttaat
     2101 tttactttca aataagtaga aatgaaaatt aatcaacttc aaatagaaat tgacggcatc
     2161 gacaaagaaa ttcttcgcga cttgatggaa gatgcacgca aacccattct tcaaatagcc
     2221 aacaaaatag gaatttcggg tgctgccatt caccagcggt tgcgaaaact agaagatgca
     2281 ggtgttattg ctggttcaaa atttatcatc gactacaaag tcttgggcta taaaaccatg
     2341 gcgtttattg gcgtttattt agacaaagca tcgagcaatt cggatgccgt tcgcgagtta
     2401 aagaaaattc ccgaagtgct agaatgtcat tatactactg gaaactggag cattctagtg
     2461 aaaattatct gcaaagacaa cgagcacttg atgcaactgc tgaacaaaaa aattcaaccg
     2521 atagacggtg tatcaagaac ggaaacgttt atttcgttgg aacaacaaat tgaaagacaa
     2581 attcagatat aagtgggaag ttggaaacag gaagttagga gttggagtca aacaaaaaac
     2641 ccaaactcct tattggaatt tggattttat atttttgaaa tttatttact taatctcttc
     2701 tgccaaatac ctgaaatgcc caatacaaca aggaagccaa agcaccaata gcggctacta
     2761 cataggttcg tgctgcccat ttcaaagcat cttcggcacc tgcatattct tgttggctta
     2821 gcatattttt gtttttcaac catgccaatg cgcgattact cgcatcatat tctaccggaa
     2881 gggttataaa actaaaggcg gtcgttactg ccattaacac caatcctata acagcaacat
     2941 aaaaaccaaa gcccaatccg gctgcggctc caagaattaa tcctccaaag attaaccatt
     3001 gcgaaagtgt ggaagaaaca ttgactaacg gcactaattt agaacgcatg gtcaacatgg
     3061 cataggctgt ggcgtgttgt actgcgtgtc cacattcgtg agcggcaacg gctgctgctg
     3121 ctgcgtttct ttggttataa accgcctcac tcaggttgac tgttttgtct gccgggttat
     3181 aatggtcggt caatcttccg ggtgttgaaa tgactctaac atcataaata ccgttgtcgg
     3241 ctagcatttt ctctgcaatt tcggcaccac tcattccgtt gcgcaaatgt acttttgaat
     3301 attcttcaaa tttgctcttc agtttggaac ttatagccca acttactaaa gcgattagtc
     3361 ctaaaagtat ataatatcct atcataattt aatttttaat aaatatagca aattgtattc
     3421 catttacaat agctgtttaa aaaatgtcaa cttgtcatct tatctacaaa ttgtaataaa
     3481 ggcaacccac tcacttgtct gataaggctt gtagttcgtg t
//
LOCUS       DAOOBH010000151         3274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_32520_length_3274_cov_4.390805, whole genome shotgun sequence.
ACCESSION   DAOOBH010000151 DAOOBH010000000
VERSION     DAOOBH010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32520_length_3274_cov_4.390805"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..393
                     /locus_tag="PK218_10885"
     CDS             136..393
                     /locus_tag="PK218_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 domain-containing protein"
                     /protein_id="HPW99058.1"
                     /translation="METASFNGLLKAILYILAFYYIFKFLARLFLPLLAKKVVEKASK
                     QFEQQQQQYQQQNQSQAQNNTSEKPKSKKVVGEYVDFEEID"
     gene            470..2917
                     /locus_tag="PK218_10890"
     CDS             470..2917
                     /locus_tag="PK218_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPW99059.1"
                     /translation="MKQLQKFYPHLLAILGFILISLVYFSPVLQGKTIYQSDIVQYTG
                     MAKEQNDFRAENHTEPYWTNSAFGGMPTYQLGAKYPNDYIGMLDDALRFLPRPADYLF
                     LYFLSFYGLLLVFRVDPLKAFFGALAFGFSTYLIIILGVGHNAKAHAIAYMPMVIAGF
                     ILVFRKRYLHGGILTMLAVALEINANHFQMTYYLFLLLLVFSVYFTYRLYQDKELKEL
                     PKIIGTFAIAVIFAIGANATNLLATKEYASFSTRSKNELTFNPNGSKVTTDNALSREY
                     ITEYSYGIAESLNLIAPRLFGGSNAENVGTDSKLYEFIINQGASPSEAEEFAENVPTY
                     WGNQPIVAAPAYIGAIVFFLAILGLFHDKRKIKYAFLTGAIFSLFLSWGKNLGFLTDF
                     FIDFVPMYDKFRAVSSIQVLLELCMPVLAIMGLQSFFIEKEDQWKSLKYTGIVTLGLV
                     VLLFLAKGMFDFTTANDNYYIKAYGEEMGSAFVNALKADRASLYAADLLRSGFFMLVA
                     FGVLWSYHKGKLVQNTAIIIIGVFMVGDLVFIDKNYVNNKDFVSKREMAEPFEATESD
                     MQILQDTTHYRVFEVSGNMSSARASFFHKSIGGYHAAKPRRMQQLFDYQIAKNNKEVL
                     DMLNIKYIIQTDEKGQEFPTMNPDANGNAWFVKELKMVNSADEEMKALDKLDTKNVAV
                     INTTEFGVWLKTEPKSFAKDSLATIKLDLYRPNHLVYTSNNTNDGFAVFSEVYYENGW
                     IATIDGKETPIHRVDYTLRGLHIPAGKHTIEFKFEPQVVKTGSMITLFSSIGMVLLII
                     GGVYFERKREKSMDNRK"
     gene            2959..>3274
                     /locus_tag="PK218_10895"
     CDS             2959..>3274
                     /locus_tag="PK218_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HPW99060.1"
                     /translation="MKPEPKKLLIITYYWPPAGGPGVQRWLKFVKYLPEFNIQPIVYI
                     PENPTYPIIDKGLESEVSDKAIILKNKITEPYGFASFFGKNKTKKISSGIIPNQKKQS
                     FLE"
BASE COUNT         1114 a          526 c          577 g         1057 t
ORIGIN      
        1 ttttaacttg aagcatgctg atttaagtat ttatttcatt cctccaaaag taatacattt
       61 tatcatttat ttaagaagac aaaccaattt ttacatcatt gaattttgta tttttgacaa
      121 aaaattattt ataaaatgga aactgcatca tttaacggat tacttaaggc aattctttac
      181 atcttagctt tttactatat tttcaagttt ttagcgcgat tatttttgcc tttattagcc
      241 aaaaaagtag tcgaaaaagc cagtaaacaa tttgaacagc aacagcaaca ataccaacaa
      301 caaaatcaat ctcaagcaca aaataacacc tcagaaaaac caaaatccaa gaaagtagtt
      361 ggagagtatg tcgattttga agaaattgat taagctgggc gttagaagac agaatctatt
      421 tatcagaaaa aaaccattaa caaaaaacta taaaccatta acttattaaa tgaaacaact
      481 tcaaaaattt tatccgcact tattagcaat actcggattt atactcattt cattagttta
      541 tttttcacct gtacttcaag gaaaaaccat ttatcaatca gatattgttc aatatactgg
      601 aatggcaaag gaacaaaacg atttcagagc cgaaaatcac accgaaccgt attggactaa
      661 ttcagcattt ggaggaatgc caacttatca attaggtgca aaatacccaa atgattatat
      721 tggaatgttg gatgatgcct tgcgttttct tcctagacct gctgattatt tgtttttata
      781 tttcttgagt ttttatgggt tacttttagt ttttagagta gatccactca aagcattttt
      841 tggagcatta gccttcggtt tctctaccta tttaatcatt attctcggtg ttgggcataa
      901 tgccaaagca catgctattg cctatatgcc aatggttata gccggattta tattggtttt
      961 caggaaacgc tatttgcatg gtggtatttt aacaatgctt gccgttgcac ttgaaataaa
     1021 tgccaatcac tttcagatga cgtattattt gttcttgctt ttgttggtgt tttcagttta
     1081 ttttacttat cgtttatatc aagacaaaga actcaaagaa ttaccaaaaa tcattggaac
     1141 atttgccata gcggtaattt ttgctatcgg agccaatgcc actaatttat tagctaccaa
     1201 agaatatgct tcttttagta cgagaagtaa aaacgaatta acttttaatc caaatggttc
     1261 aaaagttacg acggataatg ccctatcacg agaatacatc acggaataca gttatggaat
     1321 tgccgaaagt ttaaacctta ttgcgccgcg tctttttggt ggttcgaatg cagagaatgt
     1381 gggaactgac agtaaactct atgaattcat catcaatcag ggtgcatcgc caagtgaagc
     1441 agaagaattt gcagaaaatg tgccaactta ttggggaaac cagcctattg tagctgcgcc
     1501 agcctatatt ggtgcgattg ttttctttct agcaattttg ggtttatttc atgacaaacg
     1561 aaaaattaaa tatgcattcc tcacaggagc tattttctct ttattccttt cttggggaaa
     1621 aaatctcggt ttcttaactg atttcttcat tgattttgta ccaatgtatg ataagttcag
     1681 agctgtttct tccattcaag tgttactaga attatgcatg ccagttttag cgattatggg
     1741 attacaatcg ttctttatcg aaaaagaaga ccaatggaaa tccttgaaat atacaggaat
     1801 tgttacttta ggtttagtag ttttattatt tctagccaaa ggaatgttcg acttcaccac
     1861 cgccaatgac aattactata ttaaagcgta tggcgaagaa atgggaagtg cttttgtaaa
     1921 tgcgttgaaa gccgacagag ccagtttgta tgctgctgat ttattacgct ctggattttt
     1981 tatgttggtt gcttttggcg ttttgtggag ttatcacaaa ggaaaattag ttcaaaacac
     2041 agccatcatt attattggag tatttatggt tggtgattta gtttttattg acaaaaacta
     2101 tgtaaacaac aaagatttcg tgagcaaaag agaaatggca gaaccatttg aagctacaga
     2161 atccgatatg caaattttgc aagacacaac gcattaccgt gtgtttgaag tttcaggaaa
     2221 tatgagcagt gcacgagctt cgtttttcca caaatcaatt ggtggttatc atgcggcaaa
     2281 acctagaaga atgcagcaat tgtttgacta tcaaattgcc aaaaacaata aagaagtttt
     2341 ggatatgctg aacattaaat atatcattca aaccgacgaa aaaggacaag aattcccgac
     2401 gatgaaccca gatgcgaatg gtaatgcttg gtttgtaaaa gagttgaaaa tggtaaattc
     2461 tgcggatgaa gaaatgaaag ctttggataa attagataca aaaaatgtgg ctgtaataaa
     2521 taccacagaa tttggtgttt ggttaaaaac tgaaccaaaa tcatttgcaa aagacagttt
     2581 agccactata aaacttgact tataccgacc taatcattta gtatatactt caaacaatac
     2641 caatgacggt tttgcagttt tctctgaagt ttactatgaa aatggttgga tagcaactat
     2701 cgacggaaaa gaaactccaa ttcatagagt tgactacacc ttgcgtggat tacatattcc
     2761 agctggaaaa catactatcg aattcaaatt tgaaccacaa gtggtaaaaa caggaagcat
     2821 gattacattg tttagctcta tcggaatggt gttgttgatt attggtggcg tttattttga
     2881 gagaaaaaga gagaagagta tggataatag aaaatagact aaaaaattta ttttattaaa
     2941 atggtataaa cctgaaactt gaaacctgaa ccaaaaaaac ttttaatcat cacctattat
     3001 tggccaccag ctggtggacc aggcgtgcaa cgttggttaa aatttgtcaa gtatttacca
     3061 gaatttaata ttcaaccgat agtttatatt cccgaaaatc cgacttatcc gattattgat
     3121 aaaggtttgg aaagtgaagt ttcggataaa gccattattc taaaaaacaa aatcaccgaa
     3181 ccgtatggtt ttgcttcttt ttttgggaaa aataaaacta agaaaatcag ttcgggaatt
     3241 attcccaatc aaaagaaaca atctttttta gaga
//
LOCUS       DAOOBH010000152         3209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_33375_length_3209_cov_4.398224, whole genome shotgun sequence.
ACCESSION   DAOOBH010000152 DAOOBH010000000
VERSION     DAOOBH010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33375_length_3209_cov_4.398224"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /gene="creD"
                     /locus_tag="PK218_10900"
     CDS             <1..906
                     /gene="creD"
                     /locus_tag="PK218_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HPW99061.1"
                     /translation="ISEENIQWDKASIVLKTTNLKSIKSELKMQVNQEKLVFEPQNSD
                     KSKYSILATTPFDYNALLVNNKLAFNFDITYNGSNSIDFVPVGKVTTVSIDSDWKSPS
                     FDGSFAANDTSKVINDKGFHVDWKILDINRTFTQQYDDVIPNLDDYLFGVKLIETVDE
                     YQQNERVSKYGFLVIGLTFLIFFLIQSISKINIHIFQYSMIGIALIMFYTLLISITEH
                     SSFSLAYAIAGTSVVAMITLYSISILKNRKFPLFIGAALSVLYTFIFVIIQMEDYALL
                     VGSIGLFLILGAVMYFSRKIDWSKS"
     gene            complement(1040..2077)
                     /locus_tag="PK218_10905"
     CDS             complement(1040..2077)
                     /locus_tag="PK218_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HPW99062.1"
                     /translation="MKATYHKYILNFKKPSGTSRGILTEKETWFIVLEQDGKKGIGEC
                     GILRGLSIDDRNDYEEKLKWTCENIHLGEEKLWELLVEFPSIQFGIEMAFLSLRSENP
                     FELFPSKFTSGEKSIEINGLIWMGNHEEMKAQIEEKIATGFRCVKLKIGAINFEEEVA
                     LLRFIRQHFDENKIEIRVDANGGFSENEALDKINQISGFKIHSIEQPIKINQHDTMAV
                     LCEMSNLPIALDEELIGVFSTQEKEQLLDKIKPQYIILKPSFIGGFRGTLEWIALAKK
                     RNIGWWMTSALESNIGLNAIAQFTFTLNTKMPQGLGTGSLYTNNFDCPLDVENGQLWY
                     RNNLEWKVELM"
     gene            complement(2098..>3209)
                     /locus_tag="PK218_10910"
     CDS             complement(2098..>3209)
                     /locus_tag="PK218_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99063.1"
                     /translation="TNETITLKAGEKKYIKSVDFPEGTTELVLRISILDDNQKLTSSL
                     VSLLKAIPDPSGISQGTAGALFLATTITGSDKCKYAIFNNENDAKNYVYKKTIKNACI
                     IQDTPINKDAKLLNQSNSCLASNSKNVWIAFESDNWVMNQNIILEIVPWVDYNASTGW
                     TNQNKKDLINELQKQSFIKKLDNKDLFLGTFIDTFSKKYRFSEYSKLLKVEKITVIEA
                     AIKESLKASGQIASYLNFIREEAILLNSNKKPDEAIAKMQNEIITTNMATDKDFGFLG
                     NLYLSTKQFLKAEENLKKAIELNPTELTYQLKLAHVYMFTDRIQEAKDIHKKYQINSL
                     GNLKTWKEQTQFDFQQFEENGFDTSNFKKILRLLD"
BASE COUNT          961 a          526 c          519 g         1203 t
ORIGIN      
        1 atttcagaag aaaatattca gtgggacaaa gcttcaattg tattgaaaac aaccaatttg
       61 aaaagcatta aaagtgagtt gaaaatgcaa gtcaatcaag agaagttagt gtttgaacca
      121 cagaatagtg acaaatcaaa atacagtatt ttggcaacta ctccatttga ttataatgct
      181 ttactggtaa acaacaaatt ggcattcaat tttgacatca cttataacgg aagtaatagt
      241 atcgattttg taccggttgg aaaagtaact actgtttcta ttgattcgga ttggaaatcg
      301 cctagttttg atggttcttt tgcggcaaat gatacttcaa aagtgattaa cgacaaaggt
      361 tttcatgtag attggaagat tttagacatc aaccgcacgt ttacgcaaca atacgacgat
      421 gttattccca atttggatga ttaccttttt ggagtaaaat taattgaaac tgtggacgaa
      481 tatcagcaaa acgaacgcgt ttctaagtat ggtttcctag taataggttt aacattcttg
      541 attttctttt taattcaatc gattagtaaa atcaatatac atatttttca atattctatg
      601 attggtattg cgttaatcat gttttatacg ttactgattt ccataacaga gcacagtagt
      661 tttagtttgg cgtatgccat tgctggtaca tctgtcgtgg caatgattac cttgtattct
      721 atttcaatac tgaagaatcg aaagtttcca ctatttattg gtgcagcact atcggttttg
      781 tacacgttta tttttgtaat tattcaaatg gaagattatg cgctgttggt tggaagtatt
      841 ggtttgttcc taattcttgg agccgtgatg tatttctcaa gaaaaattga ttggagtaaa
      901 tcatagaaac aaccgcaaat tcgcaaatta tttcactaac cattaagttc attaaggaaa
      961 tcaagttaca aattctaaat agacttaatg gttttaacaa tagacaaact actaatttgc
     1021 gaatttgcgg tttaatatat tacatcaatt caaccttcca ctcaagatta tttcggtacc
     1081 aaagttggcc attttctaca tctaatggac aatcaaaatt gttggtatat aaacttcctg
     1141 ttcctaaacc ttgaggcatt tttgtgttga gtgtaaacgt aaattgagca atggcattta
     1201 acccaatatt gctttctaaa gcggaagtca tccaccaacc aatatttcgt tttttggcta
     1261 atgcaatcca ttctaacgta ccacggaatc cacctataaa acttggcttt aaaatgatgt
     1321 attgtggttt gattttatct aacaattgct ctttttcttg tgtggaaaac acgcctatta
     1381 gctcttcatc caaagcaatg ggcaaattgg acatttcaca cagtactgcc atcgtgtcat
     1441 gctgattaat tttaattggc tgttctatac tatgaatctt aaaaccagaa atttgattta
     1501 ttttatctaa agcttcattt tcactaaaac caccattagc atcaactcta atttcgattt
     1561 tattttcatc gaaatgttgt cgaataaatc gcaataaagc tacttcttct tcaaaattta
     1621 tagcaccaat cttcagtttt acacatcgaa aaccagttgc gattttctct tcgatttggg
     1681 ctttcatttc ttcgtgatta cccatccaaa ttaagccatt tatttcaatt gatttttcgc
     1741 cagaagtaaa ttttgaagga aacaattcaa atggattttc gcttcgtaga gacaaaaaag
     1801 ccatctcaat tccgaactga atagatggaa attcgactaa aagttcccag agtttttctt
     1861 ctcctaaatg gatattttca cacgtccatt ttagtttttc ttcgtagtca tttctatcat
     1921 caatagaaag tcctcgaaga ataccgcact cacctattcc tttttttcca tcttgctcca
     1981 aaacgataaa ccaagtttcc ttctcggtca aaataccacg agaagttccg gatggttttt
     2041 tgaagttaag aatgtattta tgataggttg ctttcattat tacttcaggt ttcagggtta
     2101 atctaaaagt cttagaattt ttttaaaatt actggtatca aagccgtttt cttcaaattg
     2161 ttggaagtca aattgggttt gttctttcca tgtttttaga tttccaagac tgttgatttg
     2221 gtacttttta tgaatgtctt tggcttcctg aattctatct gtaaacatat aaacatgcgc
     2281 caattttagc tgataggtta attcggttgg atttaattct atggctttct taaggttttc
     2341 ttctgctttt agaaattgtt tggttgacaa atatagattt cccaaaaagc caaaatcttt
     2401 atctgttgcc atgtttgtgg tgataatttc attttgcatt ttagcaatag cttcatctgg
     2461 ttttttatta gaattcaaca gtatagcttc ttctctaatg aaatttagat aggatgctat
     2521 ttgtcctgaa gcttttaaac tttctttgat ggctgcttca attactgtta ttttttcgac
     2581 ttttaataat tttgaatatt cgctgaagcg gtatttttta ctaaaggtat caataaaggt
     2641 tcctagaaat aagtctttgt tgtctaattt tttaataaat gattgtttct gtagctcatt
     2701 tattaaatct tttttatttt gattggtcca acctgtactt gcattgtaat ctacccatgg
     2761 cacaatttct agaataatat tttggttcat tacccaatta tcactttcaa aagcaatcca
     2821 aacattcttg gaattactag caaggcaaga atttgactga tttaaaagct ttgcatcttt
     2881 gttaataggt gtgtcctgaa tgatgcaagc gtttttaata gttttcttat agacataatt
     2941 ctttgcgtca ttttcgttgt taaaaatggc atatttgcat ttatcgcttc ctgttattgt
     3001 agttgccaaa aaaagtgctc cagctgtacc ttgactaata cctgatggat cggggatagc
     3061 ttttagaaga gatactaaac ttgatgttag tttttgattg tcgtctagaa tagagatgcg
     3121 taaaaccaac tcggtagttc cttcgggaaa atcgactgat ttgatgtatt ttttttcacc
     3181 tgcttttaag gtaattgttt cattagtcg
//
LOCUS       DAOOBH010000153         3170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_33865_length_3170_cov_9.025361, whole genome shotgun sequence.
ACCESSION   DAOOBH010000153 DAOOBH010000000
VERSION     DAOOBH010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33865_length_3170_cov_9.025361"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1000)
                     /locus_tag="PK218_10915"
     CDS             complement(104..1000)
                     /locus_tag="PK218_10915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPW99064.1"
                     /translation="MIPFFKSNDKSFTLLKGDCVELLNQFDFKFDMIFADPPYFLSND
                     GISVQSGKMVSVNKGEWDRSNGFEKDNEFNLNWLKACKEHLTENGTIWISGTFHNIFS
                     VAQMLTELDYKILNCITWAKTNPPPNLSCRYFTHSTEFIIWARKSKKSSHFYNYELMK
                     TINGGTQMKDVWNLPAIAPWEKSCGKHPTQKPLALLTRIILASTEKNGWILDPFTGSS
                     TTGIAANLANRKFLGIDKVDEFLSISKNRKLEIENPQIFGTYRKKLGGFEDKNQLELF
                     LAKEPEIEYERSLNLDKTTASP"
     gene            complement(1009..1278)
                     /locus_tag="PK218_10920"
     CDS             complement(1009..1278)
                     /locus_tag="PK218_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99065.1"
                     /translation="MACKKDHSRIQTIMANLPIDQGGIGRHKCAACAYDEGFNAGYNL
                     NEQINLNNLLDSLEESQAKEQRHKSPHAAYAQGYLDGVNQYYSER"
     gene            complement(1288..2127)
                     /locus_tag="PK218_10925"
     CDS             complement(1288..2127)
                     /locus_tag="PK218_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HPW99066.1"
                     /translation="MSEQFETFISQLSETNATLDYFTDFGKIKTNLSKISIKLNQLNY
                     LIGKEDLKLAIEELFEENAKVFEVLDILIAVRKNKNAKTFNREGEIVQLDTYFETSDK
                     IYEFIEDTGLGEVFRNKNITNLVDYVFGVEVGLDTNARKNRGGENMSKAVSLIFDKAK
                     IFYKKEVNSTEFPEITSLGADLKRFDFVIKTKVKTYLIETNFYNGGGSKLNEVARAYS
                     DVAPKINQYENFEFVWITDGQGWLSAKNKLEEAFSIIPSVYNLSNLDSFTQKITKEVV
                     LTF"
     gene            complement(2111..3031)
                     /locus_tag="PK218_10930"
     CDS             complement(2111..3031)
                     /locus_tag="PK218_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HPW99067.1"
                     /translation="MTIKAKPFIKWVGGKTQLIESIEKALPENFAKNKNVTYIEPFVG
                     GGAILFWILQQYPNITKAVINDINPDLTTAYKTIKETPTELVKYLQIIQDEYLPLDEE
                     KRKEYYLNKRERFNTKSLDSIENTALFIFLNRTCFNGLYRVNSKGLFNVPFGRYANPK
                     ICDKQTIFADSELLQKVEILTGDFEDTLKYATNNSFFYFDPPYKPLSETSSFNSYAKE
                     DFNDLEQIRLANFCKKIDLLGHDFILSNSDVKGKNPNDDFFDDLYEQFSIKRVFATRM
                     VNANAEKRGKLTELLITNQPSEELEYVRAI"
BASE COUNT          963 a          554 c          446 g         1207 t
ORIGIN      
        1 acgtttggca tttcgaagcg atttcctatc aagttacaac tacttttgat acgagcagta
       61 ccgcttgaaa acccactgac tgccaaatgt tttatacacg ttgttatggg ctggctgttg
      121 ttttgtctaa atttaaactt ctttcatatt caatttctgg ttctttcgct aaaaacaatt
      181 ctagttgatt tttatcctca aatccaccaa gtttttttct ataagtacca aatatttgtg
      241 gattctcaat ttcaagtttt ctgtttttag aaatactcaa aaattcatcg actttatcaa
      301 ttcctagaaa ttttctattt gcaagatttg cagcaattcc tgttgtcgaa ctacctgtaa
      361 atgggtctaa aatccaacca tttttttcag tagatgctaa aataattctt gtcagcaatg
      421 ctaaaggttt ttgtgttgga tgttttccac aagatttttc ccaaggagca attgcgggca
      481 aattccaaac atctttcatt tgagttccac cgttaatggt tttcattaac tcataattgt
      541 aaaaatgaga ggattttttg ctttttctcg cccaaattat gaactctgta gaatgagtaa
      601 aatagcgaca agaaagatta ggcggaggat ttgtctttgc ccatgtaatg caattcagaa
      661 ttttataatc caattctgta agcatttgag ctacggaaaa aatattatga aatgtaccac
      721 ttatccaaat agtaccgttt tcagttaaat gctctttaca agctttcagc caatttaaat
      781 taaattcatt atccttttca aatccattcg atcgatccca ttcaccttta tttacactta
      841 ccattttgcc gctttgaaca gaaattccgt catttgatag aaaatatgga ggatcagcaa
      901 atatcatatc aaacttaaaa tcaaactgat taagaagttc aacacaatcg ccttttaaca
      961 gtgtaaaaga tttatcatta gatttgaaaa atggaataat agccacgatt atcgctcaga
     1021 ataatattga tttacaccgt caagataacc ttgtgcgtat gcagcatgag ggcttttatg
     1081 tctttgttct tttgcttgac tttcttctaa agaatctaat aaattattta aatttatttg
     1141 ttcattaaga ttatagccag cattgaaacc ttcgtcataa gcacatgcag cacatttatg
     1201 acgaccaata ccaccttgat caattggcaa atttgccatt atagtttgaa ttcttgaatg
     1261 atcttttttg caagccataa tttttcgcta aaaagttaat actacttctt ttgtaatttt
     1321 ctgagtaaat gaatctaagt ttgaaagatt atacacactt ggtataattg aaaatgcttc
     1381 ttctaatttg ttttttgcag ataaccagcc ttgtccgtct gtaatccaca caaattcaaa
     1441 attttcgtat tgatttattt tgggggcaac atcagaatac gcgcgtgcaa cttcattaag
     1501 tttagaaccg ccgccattat aaaaatttgt ttcaatcaaa taggttttaa ccttagtttt
     1561 tatcacaaaa tcgaatcttt ttaaatctgc acccaagctt gttatctcag gaaactcagt
     1621 gctgtttact tcctttttgt agaagatttt cgctttatca aaaattaatg aaacagcttt
     1681 tgacatattt tcaccgcctc tgttttttct tgcatttgtg tcaagtccta cctcaacacc
     1741 aaaaacataa tcaacaagat ttgtaatatt tttatttctg aatacctcac ctaatccggt
     1801 atcttcaata aattcataga ttttatctga agtttcaaag tacgtgtcaa gctgaacaat
     1861 ttcaccttct ctattgaatg tttttgcatt cttgtttttt ctaacagcaa tcaaaatatc
     1921 taagacctca aaaaccttcg cattttcttc aaataattct tcaattgcaa gttttaaatc
     1981 ctcttttcct atcaaatagt tgagctgatt tagtttaatt gaaattttac ttaaattggt
     2041 tttaattttt ccaaaatcgg taaaataatc aagggttgcg ttggtttcac ttaattgtga
     2101 aataaaagtc tcaaattgct ctgacatatt ctaattcttc tgatggttgg ttggtaatta
     2161 ataattctgt tagttttcct cgtttttctg catttgcatt caccatccta gttgcaaaaa
     2221 cccttttgat gctaaattgc tcatataaat cgtcaaaaaa atcatcgtta ggatttttac
     2281 cttttacatc agaattactt aaaataaaat catgtccaag taagtcaatt tttttacaaa
     2341 aattagctaa tcgaatttgt tctaagtcat tgaaatcttc ttttgcatat gaattgaaac
     2401 ttgaagtttc acttaacggt ttatagggag gatcaaaata aaagaaacta ttatttgtag
     2461 catattttaa tgtgtcctca aaatctccag tcaatatttc tactttttgc agaagttcgc
     2521 tatctgcaaa aattgtttgt ttgtcgcaaa ttttaggatt agcgtatcgt ccaaatggaa
     2581 cattgaaaag tcctttactg tttactcgat ataagccatt gaaacaagta cggtttaaaa
     2641 agataaataa tgccgtattc tcaatggagt caagtgattt tgtgttaaac cgttcccttt
     2701 tgttcagata gtattctttc cgtttttctt catccaatgg caaatactca tcttgaatta
     2761 tttgaagata tttaactaat tcggtcggtg tttctttaat tgttttataa gcagttgtta
     2821 aatctggatt tatgtcatta ataaccgctt ttgtaatatt cgggtattgt tgtaaaatcc
     2881 agaataaaat agctcctcca ccaacaaaag gctctatata agtaacattt ttatttttag
     2941 caaaattctc aggcaaagcc ttttcaatgc tttcaattag ttgggttttt ccgccaaccc
     3001 atttaatgaa tggttttgct tttatcgtca tatttatttt ctttcttgca aatttacaat
     3061 tttattttcg gtttgcagta tgctgtctgc gtgcgttttc tcagcttgcc cataactgat
     3121 gtctgtatgc gcctgattgc ttcatattct tttgttacga tcaaataatc
//
LOCUS       DAOOBH010000154         3083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_35133_length_3083_cov_4.642668, whole genome shotgun sequence.
ACCESSION   DAOOBH010000154 DAOOBH010000000
VERSION     DAOOBH010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35133_length_3083_cov_4.642668"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1397
                     /locus_tag="PK218_10935"
     CDS             3..1397
                     /locus_tag="PK218_10935"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPW99068.1"
                     /translation="MNMFLKSTTSINLSYHNSIKLVLFRKQLMDRNVSDIINFHEYAV
                     EFVKKPINIFDSVEIARAIQNLDLPPSKIDIGKLTYEYHLEDNKYNDSRAFVIDKLKN
                     AKKFNDIKPKDVVLYGFGRIGRLLAREMMSKMGKGEQLRLRAIVTRDKNDAVLLEKRA
                     SLLRYDSVHGDFEGSVQADIENNALIINGTTVHIITANTPEEIDYTKYGIDDALLIDN
                     TGAFTTEEALKRHLVSKGVEKVLLTAPGKGVPNIVYGVNHTEYNPDEVTIYSAASCTT
                     NAITPVLAVIEETLGVVKGHIETIHAYTNDQNLVDNMHKKYRRGRSAALNMVITETGA
                     GSAVAKALPSLAGKLTSNAIRVPVPNGSLVVLNLDIAKETSFDGINSIMKKYALEGNL
                     VEQIRYSLNNELVSSDIVGSAQPSIYDSNATIVSADGKSIVLYVWYDNEYGYSHQVIR
                     LAKYIAKVRRYTYY"
     gene            1747..2121
                     /locus_tag="PK218_10940"
     CDS             1747..2121
                     /locus_tag="PK218_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99069.1"
                     /translation="MKKIIFALFLLSQVSFSQHKSNVESYSFGQNGMEIIAKSKQSIV
                     IVSTYNAKMTIREEIARKIYSMYAENKLEDNKKYTIAGNEANVTGNCIIRKKNNLIAI
                     DFYYEKVEWYSGLVEIYKKFIG"
     gene            complement(2175..>3083)
                     /locus_tag="PK218_10945"
     CDS             complement(2175..>3083)
                     /locus_tag="PK218_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPW99070.1"
                     /translation="RADGNPNTHAFVTSPEMVAALAISGRLDFNPLTDALINDNGEEV
                     MLKTPYGDELPKKGFDVEDAGFQAPAEDGSSVQVVVSPTSDRLQLLAPFAPWDGKNIS
                     GAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNMLIGAVNAFGQATNKVKNQLTG
                     EFDTVPAVARAYKAAGVPSIVVGDHNYGEGSSREHAAMEPRFLGVKAVLVKSFARIHE
                     TNLKKQGMLALTFTNEADYDKIQEDDTINFLDLTEFAPGKPLSLEFVHADGSKDIIKA
                     NHTYNEGQIGWFVAGSALNLIAAGKA"
BASE COUNT         1042 a          524 c          503 g         1014 t
ORIGIN      
        1 cgattaatat gtttctaaaa agcactactt caattaattt atcatatcat aattcaatta
       61 aattagtcct tttcagaaaa caattaatgg acagaaacgt aagtgatatc attaattttc
      121 acgaatatgc ggttgaattt gtcaaaaaac caattaatat ttttgactca gtagaaattg
      181 ctcgcgctat tcaaaaccta gatttaccac catcaaaaat agacattggc aaattaactt
      241 acgagtatca tcttgaagat aataaataca atgacagtag agcttttgtt attgacaaat
      301 taaaaaatgc taaaaaattc aatgacataa aaccaaaaga tgtagtgctt tatgggtttg
      361 gtagaatagg aagactgttg gctcgcgaaa tgatgagtaa aatgggaaaa ggcgaacaat
      421 tacgtcttcg tgctatcgtt actagagata aaaacgatgc tgttttatta gaaaaaagag
      481 cttccttact acgttacgat tcagttcatg gagattttga aggttccgtt caagccgaca
      541 ttgaaaacaa tgctttaatt attaatggca caactgttca cattattact gccaatactc
      601 cagaagaaat tgattataca aaatacggta ttgatgatgc cttattaatt gataacaccg
      661 gtgcatttac aaccgaagaa gctttgaaga ggcatttagt ttcaaaagga gttgaaaaag
      721 tattacttac tgcgccagga aaaggcgttc ctaacatcgt ttatggagtt aatcataccg
      781 agtacaatcc tgatgaagtc actatttatt cagcggcatc ttgtactacc aatgcgataa
      841 ctccagtttt agcagtaatt gaagaaacac ttggtgtagt aaaaggtcat attgaaacga
      901 ttcatgctta taccaatgac caaaacttgg tggacaatat gcataaaaaa taccgtcgtg
      961 gtcgttcggc tgcattaaac atggtcatta ccgaaactgg tgctggtagt gcagtggcaa
     1021 aagctttacc tagtcttgcc ggaaaactaa catcaaacgc cattcgtgtt cctgttccaa
     1081 atggatcttt agtcgtttta aatctagata tcgcaaaaga aacatccttt gatggtatta
     1141 attcaattat gaaaaaatat gctttagaag gcaatcttgt agagcaaatt cgttattctt
     1201 taaacaacga attggtttct tctgacatcg ttggttctgc tcaaccttct atctatgata
     1261 gtaatgcaac tatcgtttct gctgatggaa aaagtatcgt tttatatgtt tggtatgata
     1321 atgaatacgg ctacagtcat caagtaattc gactggcaaa atatattgct aaagtaagaa
     1381 gatatactta ttattagtag gtaaaaatta ttaattcata ttttaaaaaa tccgtttcga
     1441 ttgataattg aaacggattt cttttgtgtt cctttttaat caaattgctg ttttcaattt
     1501 ttttttcttc aaaataaccg ataataatac tttatttatc gataaaatgc acttttttgc
     1561 tcaaactaga agctttaaat cacaggaaaa caaaatctta atcctacaaa aaacgataat
     1621 agcacatcaa atatcgataa aaagcatgtt tttgagaaaa aagtaagtgt ttattttgat
     1681 taaagtattt tttaagcaaa attatttcta ttttgcatcg ccttttaaat ccaacacatc
     1741 tactatatga aaaaaatcat cttcgcatta ttcttgttat cacaagttag tttttctcaa
     1801 cacaagtcaa atgttgaaag ttatagtttc ggtcaaaacg gaatggaaat tatagcaaag
     1861 tcaaagcaaa gtattgtaat cgttagcacc tataatgcta aaatgacaat tcgtgaagaa
     1921 attgcaagaa aaatttattc aatgtatgct gaaaacaaat tagaagacaa taaaaaatac
     1981 accattgctg gaaatgaggc caatgttact ggtaattgta ttatccgtaa aaaaaacaac
     2041 ctcatcgcta ttgattttta ttatgaaaaa gttgaatggt attctggctt agttgaaatt
     2101 tacaaaaaat ttattggata aaaaaaatgc ctcgttactc acgaggcatt ttcatttata
     2161 agtattttag taaattacgc ttttccagca gcaatcaagt ttaaagcgga acccgcaaca
     2221 aaccaaccaa tttgtccttc gttataggtg tgatttgcct taatgatgtc ttttgaacca
     2281 tcagcatgaa caaactctaa gcttaatggt tttcctggtg caaattcggt taaatctaag
     2341 aaattgattg tatcgtcttc ttgaatttta tcataatctg cttcgttggt gaaagttaaa
     2401 gctaacattc cttgtttttt caaattcgtt tcgtgaatac gagcaaatga ttttactaat
     2461 actgctttta cgcctaaaaa tcttggttcc attgctgcgt gttctctcga agaaccttca
     2521 ccataattat gatcacccac aactattgaa ggaacgccag cagctttgta tgctcttgca
     2581 actgcaggaa ctgtatcaaa ttcgccagtt aattgatttt taactttatt tgtcgcttga
     2641 ccaaaagcat taactgctcc aatcaacata ttgttagaaa tattatctaa atgtccacgg
     2701 aaacgcaacc aaggtccggc cattgagata tggtcggttg tacattttcc aaaagctttg
     2761 attaataatt ttgcaccaga aatgtttttc ccgtcccaag gtgcaaatgg tgctaacaat
     2821 tgtaatctat ccgatgttgg agaaactaca acttgcacgc tgcttccatc ttctgctggt
     2881 gcttgaaaac ccgcatcttc tacatcgaac ccttttttag gtaattcgtc accataaggt
     2941 gtttttaaca ttacttcttc gccgttatca ttgattaatg catcggttaa tggattgaaa
     3001 tctaaccttc ctgaaattgc taatgctgcc accatttctg gcgatgttac aaaagcatgc
     3061 gtatttggat ttccatcggc acg
//
LOCUS       DAOOBH010000155         2991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_36567_length_2991_cov_7.034741, whole genome shotgun sequence.
ACCESSION   DAOOBH010000155 DAOOBH010000000
VERSION     DAOOBH010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36567_length_2991_cov_7.034741"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..>2991)
                     /locus_tag="PK218_10950"
     CDS             complement(328..>2991)
                     /locus_tag="PK218_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPW99071.1"
                     /translation="LLSEVIEKALTSDEENSDDSTLKDQMNAFKNILIHDISPKGFAD
                     VYAQTIAYGMFAARYHDPTLPTFSRQEAYELIPKSNPFLKKLFGYIAGLEVDDRIKWI
                     VDDLVNIFLACNVEEMLKNYGKSTKMEDPIIHFYEDFLSEYDPKLRKARGVWYTPQPV
                     VNFIVRAVDDILKTEFDLPQGLADTSKTKIKVDMQGKKVEQEVHRVQILDPATGTGTF
                     LAEVIKHVHTKFKGQQGIWSNYVETHLLPRLNGFELLMASYAMAHLKLDLLLSETGYK
                     PTKDQRFRVYLTNSLEEYHQDTGTLFANWLSIEANEANHIKRDTPVMCVIGNPPYSGI
                     SSNNGEWISKLIEDYKYVDGVHFNERKHWLNDDYVKFLRYGQHFIEKNGSGVLAFINP
                     HGFLDNPTFRGMRWHLLNTYDKIYTIDLHGNSKKKETAPDGSADVNVFDIMQGVSINI
                     FIKTGKKKNKELGKVFHFDLYGKRETKYDFLYDNSLKDVAFQELKPEKPFLFFVPKNQ
                     KGLKQYDNGFRVDELFSLNVTGIVTARDGFVIDFEKSVLKNRIEDFADQNQSDDFIRR
                     KYFGNKKDGKYLAGDSRGWQMTEARKKINKFNHLEIIKKVAYRIFDDRYIYYHNSMVD
                     WGREKYMYHFIEKENLGIDLCRQLVSDNYTHIFVTNKIVDDSFVSNKSRERGYVFPLY
                     IYPENKGQQTIGESTERQPNLNTEIVKQIADKLGLTFTNEKETTKDKFAPIDILDYIY
                     AVLHSPTYRVKYKEFLKIDFPRVPYPKDANTFWQLVKLGGEIRQIHLLESPTVEKYIT
                     QYPIDGDNVVTKPNYQNGKVYINDTQYFDNVPETAWNFYIGGYQPAQKWLKDRKDRKL
                     EFEDILHYQKIIVALSETDRLMKEIDKIEIE"
BASE COUNT          795 a          560 c          541 g         1095 t
ORIGIN      
        1 ttgtcaagat gatttttttg gtttggtgtc gtcttttaag ctgccgcata acggatggca
       61 gtatggttag ttgccgattt cgaagcactt tcctatcaag ttacgactac ttttgatacg
      121 ggctgtgccg ctcgaatacc cactgcaccg gcaattaact ataccgcttg ttagccgttc
      181 ggtgtttatt ttttctatca tttttctttc aatttgctac ttcgtttttg gctgccgtgc
      241 gtttgggtag gcaagctctt tggctggttt tggtcgtgtg ttggcttttc ttgcgaccag
      301 ccaatgtgct tgactacgaa gcgtgggcta ctcaatttca attttatcaa tctctttcat
      361 taacctgtct gtttccgaaa gggcaacaat tattttctga taatgcaaaa tatcctcaaa
      421 ttccagtttg cggtctttac ggtctttgag ccatttttgg gcaggttggt agccgccaat
      481 gtaaaagttc catgccgttt cgggtacatt atcaaaatac tgcgtgtcat tgatgtaaac
      541 ttttccgttt tgataatttg gtttggttac cacattatcg ccgtcaatcg ggtattgagt
      601 aatatatttt tctaccgttg ggctttccaa taaatgaatt tgccgaattt ctccacccag
      661 ttttaccaat tgccaaaagg tgtttgcgtc ttttggataa ggcactcttg ggaaatctat
      721 ttttaaaaat tccttgtatt ttactcggta tgttggcgaa tgtaaaaccg catagatgta
      781 gtctaaaatg tcaatgggtg caaacttgtc tttggttgtt tctttctcgt ttgtgaaagt
      841 taaacctaac ttatctgcta tttgcttaac aatttctgtg tttaagttgg gttgtctttc
      901 tgttgattct ccgattgttt gttgtccttt gttttcggga taaatgtata aaggaaaaac
      961 atagcccctt tctcttgact tgtttgaaac aaaagagtcg tctacaattt tatttgttac
     1021 aaaaatatgg gtgtaattat ctgaaacaag ttggcggcat aaatcaattc ctagattttc
     1081 tttttctatg aaatgataca tatatttttc tcgcccccaa tccaccattg aattatggta
     1141 gtaaatgtac ctgtcatcaa aaattctgta tgctactttt ttaataattt caagatgatt
     1201 gaatttatta attttttttc ttgcttcggt catttgccag ccacgagaat cacccgctaa
     1261 gtatttgccg tccttcttat ttccaaaata tttccttcta ataaaatcgt cactttggtt
     1321 ttgatcagca aaatcttcaa tacgattttt taaaacagac ttttcaaaat ctattacaaa
     1381 gccatctcta gcagtaacaa tacctgttac attcaatgaa aataactcat caactctgaa
     1441 cccattgtcg tattgtttca aacctttttg atttttcggt acaaaaaaca aaaatggttt
     1501 ttctggtttc aattcttgaa acgcaacatc tttcaatgag ttgtcataga gaaaatcata
     1561 tttagtttct cttttgccgt aaaggtcaaa atgaaatact ttgccaagtt ctttgttttt
     1621 ctttttaccc gtttttatga agatgttaat tgaaactcct tgcataatgt cgaatacatt
     1681 aacatctgca cttccgtcag gtgctgtttc ttttttctta ctattcccgt gcaagtcaat
     1741 agtgtaaatc ttatcataag tgtttagcaa atgccaacgc attcccctga atgtaggatt
     1801 gtccaggaaa ccgtgaggat tgatgaatgc caaaacaccg cttccatttt tttcaatgaa
     1861 atgctgtccg taacgtaaga atttaacgta atcatcatta agccaatgtt tacgttcatt
     1921 aaagtgaacg ccatcaacat atttataatc ttcaatcaat ttactaatcc attcgccatt
     1981 gtttgaagat attccgctgt atggtggatt tccaatcaca cacattacag gcgtatcacg
     2041 tttaatgtgg ttggcttcgt ttgcttcgat gcttaaccag tttgcaaata acgttcctgt
     2101 gtcttggtgg tattcttcta agctgttggt aagataaact ctaaaacgtt ggtctttggt
     2161 tggtttgtag ccagtttcgc taagtaacaa atcaagtttc aaatgtgcca ttgcataact
     2221 tgccataagc aattcaaaac cattgaggcg tggcaacagg tgcgtttcta cataattgct
     2281 ccaaattcct tgttgccctt taaatttggt gtgtacgtgt tttataactt cggcaaggaa
     2341 tgttcctgtg cctgtggcag ggtcgagaat ttgtacacgg tgaacttctt gctcaacctt
     2401 tttaccttgc atatcaactt taatttttgt tttagaagta tcggcaagtc cttgtggtaa
     2461 atcaaattca gttttaagaa tatcatcaac tgcacgaaca ataaaattta ccactggttg
     2521 cggtgtgtac cacacgccac gagctttacg taatttaggg tcgtactcgc tcaaaaaatc
     2581 ttcgtaaaaa tggataattg ggtcttccat tttagtagac ttaccgtagt ttttaagcat
     2641 ttcttcgaca ttacacgcaa ggaatatgtt tactaaatca tcaacaatcc attttatgcg
     2701 gtcgtcaact tctaaacctg caatatatcc gaatagtttt ttaagaaaag ggtttgattt
     2761 tggaatgagt tcgtaggctt cttgtcggct aaatgtaggt aatgttgggt cgtgataacg
     2821 agcggcaaac ataccatacg caatagtttg agcatataca tcggcaaaac ctttgggaga
     2881 aatatcgtga ataagaatat tcttaaaggc attcatttgg tctttaagag tactgtcgtc
     2941 gctgttttct tcgtcgcttg taagtgcttt ttctataact tcggaaagaa g
//
LOCUS       DAOOBH010000156         2924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_37637_length_2924_cov_6.226908, whole genome shotgun sequence.
ACCESSION   DAOOBH010000156 DAOOBH010000000
VERSION     DAOOBH010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37637_length_2924_cov_6.226908"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PK218_10955"
     CDS             complement(<1..1051)
                     /locus_tag="PK218_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPW99072.1"
                     /translation="MEELIQFRHELHKHPEVSGNEKQTAQRVLAFLSNYPPSEMITQL
                     GGEGILAIYNGKQTGTSILFRCELDALPIQEINTFGYKSVSHGVSHKCGHDGHTAILC
                     GMAKTLYEKPLEKGTVMLLFQPAEEDGNGAKSVLNDSKFNTIKPDFVFALHNLPGFKK
                     HQIIVKENTFTCAVNSMIIQLNGKTSHAGEPENGINPALAIAEIIQAFNSKINTDVSS
                     KKYCLITPIHINMGEKAYGVSAGYGEIHFTIRSNSNAQMRIIESNLEEKVNEIASKYN
                     LKTKIDWTESFQANENNSEAVKYIRKATLVQNLEILEKNLPFTFGEDFGLFTQQFKGA
                     MFGIGAGKNTPALHNP"
     gene            complement(1057..1491)
                     /locus_tag="PK218_10960"
     CDS             complement(1057..1491)
                     /locus_tag="PK218_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW99073.1"
                     /translation="MEFKIINPETGENSTYTHQIIAEFLYTHLEQYGDKIEDILKCIN
                     YAMNPEKGGSIIVGLDDSKIVGVVILNNTGMSGFIPENILVYIAVDNSQRGKGFGKKL
                     MQKAIDTVDGNIALHVEPDNPAKGLYEKLGFTNKYLEMRLTK"
     gene            1768..>2924
                     /locus_tag="PK218_10965"
     CDS             1768..>2924
                     /locus_tag="PK218_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPW99074.1"
                     /translation="MLIKVFGSAVFGVEATTITVEVNIDKGIGYHLVGLPDNAIKESS
                     YRIAAALKNNGYELPGKKITINMAPADLRKEGSAYDLTLAIGILAASGQIKADEVDKY
                     LIMGELSLDGGLQPIKGALSIAIKAKEEGFKGFFLPIQNVKEAAIVSGLDVYGVENVR
                     QVIDFFDGKETLEPTQINTREEFYKTLDFPEFDFSDVKGQESIKRCMEIAAAGGHNII
                     LIGPPGSGKTMLAKRLPSILPPMTLHEALETTKIHSVAGKVKDTGLMNQRPFRNPHHT
                     ASSVSLVGGGSYPQPGEISLAHNGVLFLDELPEFKREVLEVMRQPLEDREVTISRAKF
                     TITYPSSFMLVASMNPSPGGYFNDPDAPVSSSPAEMQRYLSKISGPLLDRI"
BASE COUNT          948 a          491 c          525 g          960 t
ORIGIN      
        1 cgggattgtg taaagccgga gtatttttgc cagcaccaat accgaacatt gcgcctttga
       61 attgttgggt aaacaatcca aaatcttcac cgaaagtaaa aggaaggttt ttttctaaaa
      121 tttctagatt ctgaactaaa gttgcttttc ttatgtattt aacagcttca ctattatttt
      181 cattcgcttg aaaactttct gtccaatcga tttttgtttt caaattatat ttagaagcaa
      241 tttcgttaac tttttcttct aaatttgatt ctataattct catttgggca ttgctattgc
      301 ttctgatggt aaaatgaatt tcgccataac cagcagaaac accataggct ttttcgccca
      361 tattaatatg aataggagtg atgagacaat actttttaga agaaacgtca gtattgattt
      421 tggaattaaa agcctgaata atttcggcaa tagccaacgc aggattgatt ccgttttcgg
      481 gttcgccagc gtgagaagtt tttccgttta attgaataat catactgttg acagcacaag
      541 taaatgtatt ttcttttaca atgatttgat gttttttaaa tcccggaagg ttatgcaaag
      601 caaaaacaaa atcaggtttt atagtattga atttactatc gttcaaaaca ctttttgctc
      661 catttccatc ttcttctgca ggttgaaata aaagcataac agttcctttt tctagaggtt
      721 tttcataaag ggttttcgcc attccacata aaatagccgt atgaccatcg tgaccacatt
      781 tatgggaaac accatgagaa actgatttat aaccaaacgt attaatttct tgaataggca
      841 aagcatccaa ttcacagcga aatagaattg aagttcctgt ttgttttccg ttataaatgg
      901 ccaaaattcc ttcgccacca agttgagtaa tcatttcact aggcggataa ttagaaagaa
      961 aagctaaaac gcgttgagcc gtttgttttt catttccaga aacttcagga tgtttgtgta
     1021 gttcgtgtct gaattgaata agttcttcca aaacgattat tttgttaaac gcatttctaa
     1081 atatttgtta gtaaaaccaa gcttttcgta taatcctttt gcaggattat ctggttctac
     1141 gtgtagcgca atatttccat caacggtgtc aattgctttt tgcattagtt ttttaccaaa
     1201 accttttcca cgctggctgt tatcaaccgc aatgtaaacc aagatgtttt cgggaataaa
     1261 tccgctcatc ccagtgttgt ttaaaattac cacaccaact atttttgaat catctaaacc
     1321 tacaattatg cttccgcctt tttcgggatt catggcatag ttgatgcatt ttaaaatgtc
     1381 ttctatttta tcgccatatt gttctaaatg agtgtataag aattcagcaa taatttgatg
     1441 tgtataagtc gaattttctc cagtttccgg attaatgatt ttaaattcca ttttgattta
     1501 ataaatttta aagatgttaa ccgatatgga ttttccaaag tggttgtaga tgcaacgtgt
     1561 tgcaaaagat ggaaacaaga ttgatttaag attaaaatca aagaaaaatt ctaaaaaaga
     1621 attttcttgt tttcaaaaaa tatttttgca aagataagga ttttaaaata aaattatttt
     1681 ttgtactaaa aaagctaaac taaagcttaa aaaatcattt aatttttatg atatttgttt
     1741 taaaaatata ccaaaaaata aaatgctatg ctaataaaag tatttggaag tgcggttttt
     1801 ggagtggaag ccacaactat taccgttgaa gtaaatattg ataaaggaat tggttatcat
     1861 ttagtaggat tacccgataa tgccattaaa gaaagcagct atcgaattgc agcagcatta
     1921 aaaaataacg gctacgaact gcctggaaaa aaaattacca taaacatggc accagccgat
     1981 ttgcgcaagg aaggttcggc ctacgattta accctagcca tcggaatttt agccgcatct
     2041 ggacaaataa aagccgatga agttgataaa tacttaatca tgggcgaatt gtctcttgat
     2101 ggtggtttgc aaccgataaa aggtgctttg tctatcgcca tcaaagccaa agaagaaggc
     2161 ttcaaaggat tttttctacc cattcagaat gtaaaagaag ccgctatagt ttcgggtttg
     2221 gatgtttacg gtgttgaaaa tgtacgccaa gtgattgatt tttttgatgg aaaagaaact
     2281 ttagagccaa cacaaattaa tactagagaa gagttttata aaaccttaga ttttcctgag
     2341 tttgattttt cagatgtaaa aggacaggaa agtattaaac gatgtatgga aattgctgct
     2401 gctggtggtc ataatatcat tttaattggt cctccaggtt caggaaaaac gatgcttgcc
     2461 aagcgattgc caagtattct gccaccaatg acacttcatg aagctttaga aaccaccaaa
     2521 attcatagtg tagctggaaa agtaaaagat accggtttaa tgaaccaacg accgtttaga
     2581 aatccacatc acacggcaag ttcagtttcc ttagttggcg gtggaagtta tccgcaacct
     2641 ggagaaattt cgttggcaca caatggcgtt ttatttttag acgagttacc agaatttaaa
     2701 cgagaagttc tcgaagtaat gcgccaacca ttggaagata gagaagtaac catttcgaga
     2761 gcaaaattca ccattactta tccatcgtca tttatgttgg tggcgagtat gaacccaagt
     2821 ccaggtggtt atttcaacga tcctgatgcg ccagttagtt catctccagc agaaatgcag
     2881 cgctatttga gtaaaatttc gggaccatta ttagaccgaa ttga
//
LOCUS       DAOOBH010000157         2834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_39242_length_2834_cov_5.598417, whole genome shotgun sequence.
ACCESSION   DAOOBH010000157 DAOOBH010000000
VERSION     DAOOBH010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39242_length_2834_cov_5.598417"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PK218_10970"
     CDS             <1..301
                     /locus_tag="PK218_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HPW99075.1"
                     /translation="YAGITDYEFHYSLYETGDFYKKHLDQFKDNASRQFSMISYLNPN
                     WETQHGGELQIYQTDANQTISPTQGKMVFFKSDQLEHEVLVTHARRMSVTGWLKR"
     gene            complement(398..811)
                     /locus_tag="PK218_10975"
     CDS             complement(398..811)
                     /locus_tag="PK218_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HPW99076.1"
                     /translation="MKRTATAVWSGTGKDGNGNLTTQSGVLKATQYSFGSRFEEGIGT
                     NPEELIAAAHAGCFTMKLAFNLQTEGFAATELKTIGQIVLEDGRITQSNLTLTARVDG
                     ISDAQFADLVKDAEENCPISKLLNTTISVTHTLNA"
     gene            948..2702
                     /locus_tag="PK218_10980"
     CDS             948..2702
                     /locus_tag="PK218_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPW99077.1"
                     /translation="MPHTLSSMLLKTTTLFLLIFQFCVGTFVAQTPKEEFDKKNKEFF
                     QLVWNDTKQAKTTLKWLEEFAKEKNKPKFYSKYFNAKATLFFANSNYTDAVTNYLKAY
                     DYAKKSKDKWQQGIVLNNLGGCLYQSQEYKKAKEYFDIAYQLFVEIDDKDWQTNVLYN
                     LAIINFDLENYTEAEKQFLLLEKTYLQDNNPSYAGYCNRVIGTIYIYTNRLDEAIKQL
                     ELSLKRSIPEDDLDSYSLSLQKMGHALLETGKNQEALSYLHKSLVITKANQFNKWTLI
                     NYELMAQTFWKSKIPDSAYFYYEKSLAYKDTVFNETKQAEFATLETQFKTKLKEEKII
                     QQKTTIDHKNQIIYGLVFFIIIIIVLLLLFIITRLKLLKSRRKLQKSLDEKESLLREI
                     HHRVKNNLQVVSSLLNMHVRKVTDPDSKKILEEGSDRLIAMSLIHNNLYPHANLKNIS
                     LDEYLFKLSHQLFDNYKLNYATVKLNTELEKIEVDIDKLIPIGLIVNELISNAMKHAF
                     HEASEAEISITLKYANTNEIELEVADNGQGIASNWHEKETDSIGMKLITIFSEKLKSK
                     LSIENTNGTKIKLIIPTL"
BASE COUNT         1043 a          512 c          444 g          835 t
ORIGIN      
        1 ctatgcgggc atcaccgact acgaattcca ttactcgctc tacgaaacag gcgatttcta
       61 caaaaaacat ttagaccaat tcaaagacaa cgccagccga caattctcaa tgattagcta
      121 cctaaaccct aattgggaaa cccaacacgg tggcgaacta cagatatatc aaacggatgc
      181 caaccaaacc atttcgccta ctcaagggaa aatggtgttc tttaaaagtg accaactcga
      241 acatgaagta ttggtaaccc atgcgcgtag aatgagtgtt accggatggt taaagcgatg
      301 acactttaat taggtattaa taaataaatt cacaaaaaaa aacagcaaac ccaaatgact
      361 tgctgttttt catataacgt aaaaaccgaa accttactca ggcattcaaa gtatgcgtaa
      421 ccgaaatagt agtgttcaat aatttcgaga tgggacaatt ttcttcagca tcttttacta
      481 aatcggcaaa ctgcgcatcc gaaatgccat ctactcttgc tgttaacgtt aagttagatt
      541 gggtgatcct gccatcttct aaaactattt gtccaatagt tttcaattcc gtagccgcaa
      601 aaccttccgt ttgaagatta aacgccaact tcatcgtgaa acaaccggca tgtgctgctg
      661 ctattagttc ctcaggattc gtaccaatgc cttcttcaaa tcgggaacca aacgaatatt
      721 gtgtcgcttt taatacgccg ctttgtgtgg ttaagtttcc gtttccgtct tttccagtgc
      781 cgctccaaac ggcagttgct gttcttttca tactaaagtc ttttttaatt tctataaaat
      841 taagaaagtt tcaatgtttt tatattagga ttttgttaaa tgtaggttta ttattgagac
      901 tatttaaaat taaaataaaa atgtaacttt actttttgat tctaaaaatg ccacacacac
      961 tatcatccat gctattaaaa acaacaaccc tctttttatt gatatttcag ttttgtgttg
     1021 gtacatttgt cgcacaaacg ccaaaagaag aatttgataa gaaaaacaaa gaattttttc
     1081 aattggtttg gaatgataca aaacaagcaa aaaccacatt aaagtggttg gaagaatttg
     1141 ccaaagaaaa aaacaaaccc aagttttatt caaaatattt taatgcaaaa gctacgctat
     1201 tttttgcaaa ctcaaattat accgatgccg tcaccaacta tttaaaagcc tatgattatg
     1261 ccaaaaaatc aaaagacaaa tggcaacaag gtattgtttt aaataatcta ggcggttgtc
     1321 tttatcaatc ccaagaatac aaaaaagcga aagagtattt tgatattgct tatcagttat
     1381 ttgtagaaat tgacgataaa gattggcaga ccaatgtttt gtacaatttg gcaatcatta
     1441 attttgattt agaaaattat actgaagccg aaaaacaatt cctattatta gaaaaaacct
     1501 atcttcaaga caataaccca agttacgctg gatactgtaa cagagttata ggaaccatct
     1561 acatttacac caacagattg gatgaagcaa taaaacaatt agagctttct ttaaagagaa
     1621 gtattccaga ggatgattta gattcgtatt cactttcgtt acaaaaaatg ggacatgcct
     1681 tgttggaaac gggtaaaaac caagaagcac tatcctatct tcacaaaagt ttagtcatta
     1741 ccaaagccaa tcaattcaac aaatggacac ttatcaatta tgagttaatg gcgcaaacgt
     1801 tctggaaaag taaaattccc gatagtgcct atttttacta cgaaaagagt ttggcctaca
     1861 aagacaccgt ttttaatgag accaagcaag ccgaatttgc taccttagaa acacaattta
     1921 aaaccaaact caaagaagaa aaaataatac aacaaaaaac taccattgac cataaaaacc
     1981 aaattattta tggattggtt ttttttatca tcattatcat agtattgttg ttactattca
     2041 ttatcacccg tttaaaactt ttaaaaagca gacgtaaact acaaaaatcg cttgatgaaa
     2101 aagaatctct acttcgcgaa attcatcatc gagtaaaaaa caatctacag gtggtttcta
     2161 gtttgctcaa catgcatgtt agaaaagtaa ctgacccaga ctctaagaaa atattagaag
     2221 aaggttcgga tagactaata gcaatgtcac tcattcataa taatttatat ccacacgcca
     2281 acctaaaaaa catttctttg gatgagtatt tattcaaatt aagccatcaa ctttttgata
     2341 attataaact aaactatgct acggttaaat taaacaccga attagaaaaa attgaagttg
     2401 acattgataa gcttatccct attggtctta tcgtaaacga acttatttca aatgccatga
     2461 aacatgcctt tcatgaagcg tctgaggctg agataagcat tacgcttaaa tatgctaaca
     2521 cgaatgaaat tgaacttgaa gttgccgata atggtcaagg gattgctagt aattggcacg
     2581 aaaaagaaac cgattcaatt ggcatgaagt taataacaat attcagtgaa aaattaaaaa
     2641 gtaaactctc catagagaat acaaatggta ctaaaatcaa actcataatt ccaactttat
     2701 gatagccgtt ttaattgtag aagatgagcc attaattgcc gaagacatta agcaaacctg
     2761 tttggaaaac aaaatagagg tagccggtat agcttattct ttagaggatg ccattactgt
     2821 tttagaggaa aaaa
//
LOCUS       DAOOBH010000158         2721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_41342_length_2721_cov_5.955739, whole genome shotgun sequence.
ACCESSION   DAOOBH010000158 DAOOBH010000000
VERSION     DAOOBH010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41342_length_2721_cov_5.955739"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..876)
                     /locus_tag="PK218_10985"
     CDS             complement(175..876)
                     /locus_tag="PK218_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HPW99078.1"
                     /translation="METEIPAIFRARETILPERFTFHSSRMRMKKVTKEELEAMDAMS
                     LKCAQELSDAHVDVMGYACLVAIMSMGRGYHCVSEVNLHQETVANDFPTPIVTSAGAL
                     INGLNVLGAKQISIITPYMRPLTDMVVDYIEHQGIKVKESIALEIPDNLEVAAQNPMN
                     LLEIYKQLDLKDVDVLVVSACVQMPSLEAIDLIQAECGIPVTSAAVCTTYEMMKKLSI
                     EAKSPIGGELLNGKY"
     gene            complement(1025..1939)
                     /locus_tag="PK218_10990"
     CDS             complement(1025..1939)
                     /locus_tag="PK218_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPW99079.1"
                     /translation="MKLLTYKTNETESRLGFLHNNQVIDMEDFGEISNFPLPSTMLEL
                     IDMGYELVEELNDMVAETDQKLLDEIAYEIGEVIFLAPIPKPRKNIIGIGLNYTEHVA
                     ESARTLDTSNELPQQPIIFSKPPTTVTATNTEILLNRKLTNQLDWEVELAVVISKKGK
                     YVSKADAMDYVFGYTIINDISARDCRRSGQWIVSKGQDTFAPMGPILVTKDEIENPHN
                     LNLSLTVNGIEKQNSNTKLMLFNINDLIEDLSTVFTLEAGDIIATGTPAGVGAGRNPQ
                     EWLKDGDVVKCTVEGIGSIVNTVKEIAP"
     gene            complement(2042..>2721)
                     /locus_tag="PK218_10995"
     CDS             complement(2042..>2721)
                     /locus_tag="PK218_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW99080.1"
                     /translation="VNDFVITPNSTWHEHGVDANGKMCIWQDGLDIPLVNALEANDYA
                     VYDGKQPLEQEINYSPLAYGCAGLLPADVTWNKPYSPLFKYSWKEVYPTLLETLKVKP
                     INPIDGIIMQYSNPLNGGHVMQTMGASMQLLPAGFKGKAHKHTGSIVYQCAKGSGYSI
                     INGKRFEWKERDIFCVPSWAWHEHHNASENEDACLFSFNDLPVIEKLGLYQEKELTEN
                     KGHQQVQ"
BASE COUNT          788 a          510 c          447 g          976 t
ORIGIN      
        1 aataatctcg aaatttagtt ttcgagtttt tgtgtgtgat tgttttttta attatgatgt
       61 aaactagcat gataatcggg acatgcaatg caaaatatac tccatgaaga cctactagcg
      121 ttacaccaat tgcaataaat aaaagaaatg caattttatg tttaattccg tttatcaata
      181 ttttccattt aacaattcac caccaatagg agattttgct tcgatactga gttttttcat
      241 catttcataa gtggtgcaaa ctgctgctga agtcacagga attccgcatt ctgcctgaat
      301 taagtcaatc gcttccaaag atggcatttg tacacaagca gaaacgacta aaacatccac
      361 atctttcaaa tccaattgct tatagatttc tagtaaattc attgggtttt gtgcagcaac
      421 ttctagatta tcaggaattt caagtgcaat actttctttt actttaatgc cttgatgctc
      481 aatataatcc acaaccatat ccgttaaagg tcgcatatac ggagtgatta ttgaaatttg
      541 ttttgcacct aaaacattta aaccatttat taatgctcca gcagaagtta caattggagt
      601 agggaagtcg ttggcaaccg tttcttggtg tagattcact tctgaaacac aatgataacc
      661 tcgacccata ctcataatag caaccaaaca agcataaccc ataacatcaa catgcgcatc
      721 agaaagttct tgagcacatt tcaaactcat ggcatccatg gcttcgagtt cttctttggt
      781 tacttttttc attcgcattc tactgctgtg aaacgtaaaa cgttctggca aaatggtttc
      841 tctagctcta aaaatagctg gtatttcggt ttccatcgtg acgtttgatg atggaactat
      901 ttggcctatt ctgtatttaa gcctccccaa cccctccaaa ggagggactt tttgtgtttc
      961 atttatcatt ttttttttaa atcaaattat tttttttaaa agctagtaat tccccctttg
     1021 ggggttaggg ggctatttct ttaactgtat tcacaatcga gccaattcct tcaacagtac
     1081 attttacaac atcaccgtct tttaaccatt cttgagggtt tcttcctgct ccaactccag
     1141 ctggagttcc agtggcaata atatcgccag cttccaaagt aaaaacggta cttaagtctt
     1201 caatcaaatc gttgatgttg aataacatta atttggtatt ggaattttgt ttttcaatac
     1261 cgttcactgt taatgataaa tttaaattat gtggattttc aatttcgtcc ttggtgacta
     1321 aaattggtcc cattggtgca aaagtatctt gtccttttga aacaatccat tgtccagaac
     1381 gacgacaatc tcttgctgaa atgtcattga ttatagtata tccaaaaaca taatccattg
     1441 cgtctgcttt agaaacgtat ttcccttttt tgctaatcac tacagccaat tcaacttccc
     1501 aatccaattg attagttaat tttctattca atagaatttc agtgtttgtt gctgtcacag
     1561 ttgtaggtgg ttttgaaaaa ataatgggtt gttgtggtaa ttcatttgat gtgtctaatg
     1621 ttctcgcgct ttcagcaacg tgttcggtat aattcaaacc aattccgatg atgttttttc
     1681 gcggtttagg aataggagca aggaagatta cttcgcctat ttcgtaagca atttcatcta
     1741 ataatttttg gtctgtttca gcaaccatgt cgtttaattc ctcgaccaat tcatagccca
     1801 tatcgattaa ttctaacata gttgatggta atggaaaatt agatatttca ccgaagtctt
     1861 ccatgtcgat tacttgattg ttgtgaagga atcctaagcg tgattctgtt tcgtttgttt
     1921 tgtaagtaag tagtttcatt ttatttgttt aaccgcgaag aacgcaaaga tttacgcaaa
     1981 gttcgcaaag ttttttattg aatttgattt tagaacatgt acttttcttg cactataaaa
     2041 gttattgtac ttgttgatgt cccttattct ctgttaattc cttttcttga tacaaaccta
     2101 gtttttctat tacaggtaaa tcattaaacg aaaataaaca ggcatcttca ttttctgatg
     2161 cattatgatg ttcgtgccaa gcccaacttg gaacacagaa aatatcgcgt tctttccatt
     2221 caaaacgttt tccgttgatg atggaatagc cgcttccttt ggcacattgg taaacaattg
     2281 aaccagtatg tttgtgtgct tttcctttga atcctgcggg taataattgc atacttgcac
     2341 ccatagtttg catgacatgt ccgccattta atggattgga atactgcatt ataattccat
     2401 ctattggatt gataggttta acttttaatg tttctaataa agttggatac acttctttcc
     2461 atgaatattt gaaaagtggc gaataaggtt tgttccaagt tacatctgca ggtagtaaac
     2521 cagcgcaacc ataagctaat ggagaataat taatttcttg ttccaatggt tgttttccat
     2581 cataaactgc gtagtcattt gcttctaaag cattcaccaa tggaatatcc aaaccatctt
     2641 gccaaataca catttttccg ttagcatcaa ctccgtgttc atgccaagta gaattgggtg
     2701 ttatcacaaa atcatttact t
//
LOCUS       DAOOBH010000159         2696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_41882_length_2696_cov_5.861416, whole genome shotgun sequence.
ACCESSION   DAOOBH010000159 DAOOBH010000000
VERSION     DAOOBH010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41882_length_2696_cov_5.861416"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1130
                     /locus_tag="PK218_11000"
     CDS             54..1130
                     /locus_tag="PK218_11000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPW99081.1"
                     /translation="MRAAAILMVLFGHCLWIFPQSNNTISQILQLFGFFGVEVFFVLS
                     GFLIGNILLKLFIQNTFTFKTALHFLKRRWFRTLPNYFLILIINVIIAFLIGYQIPQT
                     WHYFLFLQNFNTTMQPFFPESWSLSVEEFAYIFLPILLVITNSLAKKNKSTLYIISVL
                     ILIIASFLAKINYTQTTTNISLDQWNLSLKSVVIYRLDSIYIGVLASWLYNKYSNNWE
                     KLKYTSMILGVLLFFFLFFGLGKLGLLIETHHYFWNVLYLPLTSICVALFLPLLSLWK
                     TEITTIQRLIEFISKISYSIYLLHYSLILYLMKEFIDTTSLSSINLYLFTALYLTIVF
                     ILSALLYQFYEKPMMDIRDKINNS"
     gene            1286..>2696
                     /locus_tag="PK218_11005"
     CDS             1286..>2696
                     /locus_tag="PK218_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HPW99082.1"
                     /translation="MAFDIEMIEKVYANLATRVDKARELVGRPLTLSEKILYSHLWEG
                     MPSQAFTRGKDYVDFAPDRVACQDATAQMALLQFMHAGKKTVAVPTTVHCDHLILAKD
                     GAKKDLALANDQSKEVFDFLSSVSDKYGIGFWKPGSGIIHQIVLENYAFPGGMMIGTD
                     SHTVNAGGLGMLAIGVGGADAVDVMSGMGWELKFPKLIGVKLTGKLSGWTAPKDVILK
                     VADILTVKGGTGAIVEYFGEGATSMSCTGKGTICNMGAEIGATTSTFGYDDSMRRYLS
                     ATGRQDVVDAADKVASYLTADAEVYANPEQYFDQLIEINLSELEPHINGPFTPDRGTP
                     VSQMKEEAAKNEWPIKVEWGLIGSCTNSSYEDMSRAASIVRQAVEHGITPKAEFGINP
                     GSEQIRFTIERDGIIADFEKMGTKVFTNACGPCIGQWDRAGADKQEKNTIVHSFNRNF
                     SKRADGNPNTHAFVTSPEMV"
BASE COUNT          907 a          500 c          453 g          836 t
ORIGIN      
        1 actaaccgat gcaaaaccaa acaaaatcct ccagaatctt cggtctcgat ctaatgcgag
       61 ccgccgcaat tcttatggta ttgttcgggc attgcctttg gatattcccc caaagcaaca
      121 atacaatttc gcaaatacta caacttttcg gcttctttgg cgtcgaagta ttctttgtac
      181 taagcggttt cttaatcgga aacatactgc tcaaactttt tattcaaaat acattcacgt
      241 tcaaaacagc cttacacttt ctaaaaagac gttggtttag aaccttgccc aattattttt
      301 taatattaat tatcaacgta atcatagcat ttctaatagg ctatcaaatt ccccaaacct
      361 ggcactattt tctctttctg caaaacttca acaccaccat gcaacccttc tttcccgaat
      421 catggagttt atccgtagaa gagtttgcct atatttttct gccaatacta ttagtgataa
      481 ccaacagtct cgccaaaaaa aataaaagta cattatatat aatttccgtt ttaattctaa
      541 ttatagcctc ctttttagcc aaaataaact atactcaaac tacaacaaat atatccttag
      601 accaatggaa cctttcgcta aaatcagtag tcatctatcg gttagattcc atttatattg
      661 gcgttttagc aagctggttg tataataaat attccaacaa ttgggaaaaa ctaaaatata
      721 catccatgat tctcggagtg ctattgtttt tcttcctctt tttcggttta ggaaaattag
      781 gtctattaat tgaaacacac cactattttt ggaatgtact ctatttacca ctaacctcca
      841 tttgtgttgc attatttttg ccattgcttt ccctttggaa aacagaaata acaaccattc
      901 aaagactaat tgaattcatt agcaagattt cctactccat ttatttactt cattacagcc
      961 tgattctcta tcttatgaag gaatttatag atacaacttc cctttcttca atcaatttat
     1021 acctattcac agcactttat ttaacaattg tattcatttt gagtgcatta ttatatcaat
     1081 tttacgaaaa accaatgatg gatattagag ataaaataaa taattcttaa tttttaggtc
     1141 tttaaatccg atattgacat aaaagcaata cccaaattca ttttttcaca aatcatcatc
     1201 tttttttaag ataatctata tttatttttt atttggtaca tcgatttcgt aaatttgcaa
     1261 cacaaataaa taaacctatt ttattatggc atttgatatt gaaatgattg aaaaagtgta
     1321 tgctaaccta gcaacaagag ttgacaaagc tcgcgaacta gttggtcgtc cacttacctt
     1381 atctgaaaag attttatatt cacacctttg ggaaggaatg ccttctcaag cgttcacaag
     1441 aggaaaagat tatgttgact tcgctccaga tagagttgct tgtcaagatg caaccgctca
     1501 aatggcatta ttgcaattca tgcacgcagg aaaaaaaact gtagcagtac caacaacagt
     1561 acattgcgac catttaattc tagcaaaaga cggtgcaaaa aaagatttag cactagccaa
     1621 tgaccaatca aaagaagtat ttgattttct ttcttccgtt tccgataaat atggaattgg
     1681 attctggaaa ccaggttcag gaattattca tcaaattgtt ttagaaaatt atgctttccc
     1741 aggcggaatg atgattggaa ccgattcaca tactgtaaat gcaggtggat taggaatgtt
     1801 ggcaattggt gtaggtggag cagatgctgt agatgtaatg tcaggaatgg gttgggaatt
     1861 aaaatttcca aaactaatag gagtaaaatt aacaggtaaa ctttcgggtt ggacagcacc
     1921 aaaagatgta attctaaaag ttgccgatat tcttactgtt aaaggtggaa caggtgccat
     1981 tgttgaatat tttggagaag gtgctacgtc aatgtcgtgt acaggaaaag gaactatttg
     2041 taacatgggt gccgaaattg gagcaaccac ttcaaccttt ggttatgatg attcgatgag
     2101 aagatattta tcagctacag gaagacaaga tgttgttgat gcagccgata aagtagcgag
     2161 ttacttaacc gccgatgccg aagtatatgc aaatccagaa caatatttcg accaattaat
     2221 agaaatcaat ttatcagaat tagaaccaca tatcaacgga ccatttacgc cagatagagg
     2281 aacacctgtt tctcaaatga aagaagaagc agctaaaaat gaatggccaa taaaagtaga
     2341 atggggatta atcgggtctt gtacaaactc ttcttatgaa gacatgtcga gagcagcctc
     2401 tattgttcga caagcagttg agcacggaat tactcctaaa gctgaattcg gaatcaatcc
     2461 tggttctgag caaattcgtt tcactattga aagagacgga atcattgccg attttgaaaa
     2521 aatgggaaca aaagtattta ccaatgcttg tggaccatgt atcgggcaat gggatagagc
     2581 aggagcagac aaacaagaaa aaaacaccat cgtacactca tttaacagaa acttctcaaa
     2641 gcgtgccgat ggaaatccaa atacgcatgc ttttgtaaca tcgccagaaa tggtgg
//
LOCUS       DAOOBH010000160         2667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_42459_length_2667_cov_7.333461, whole genome shotgun sequence.
ACCESSION   DAOOBH010000160 DAOOBH010000000
VERSION     DAOOBH010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42459_length_2667_cov_7.333461"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PK218_11010"
     CDS             <1..793
                     /locus_tag="PK218_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99083.1"
                     /translation="LIDIRQKINGLIIMLLKRKTTKCDHVEKFTKYLINKKEEQKKDS
                     FAIISTNWDILIDNSFKRNSKGVIDYCCHTTPYSGQNMPALVALTKNLFTIKLLKLHG
                     SMNWLQCKRCQRLFVTFFEKIAIDEYINKPICKFCKENFKDENANGGALLSSQLIMPT
                     FLKDLNNVQLKLIWQNAGIELSEASSIVFMGYSFPTADFELRQLLARNVRHSAKIEVV
                     LCPSDKPTNDKDTNSPENRFKSFFGKREVNFYYEGVEEYIKTNYT"
     gene            900..1652
                     /locus_tag="PK218_11015"
     CDS             900..1652
                     /locus_tag="PK218_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPW99084.1"
                     /translation="MEAKQDLKLAVIIDADNVPSENVKGMMEEIAKYGIPTFKRIYGD
                     WTKPNLASWKKVLLENAITPIQQYEYTTGKNSTDSAMIIDAMDILYTGKVDGFCIVSS
                     DSDFTRLATRLRESGMKVLGFGEKKTPNPFIVACDKFIYLEILKRPISAGKQKTENSQ
                     KAKHETIKQIDLEIIFLIASTVNDVADEDGWAFLGDVGNLLIKKMPDFDPRNYGFEKL
                     TPLIKSLKSNFDIDERTTERASIKLVYIKTKK"
     gene            1748..>2667
                     /locus_tag="PK218_11020"
     CDS             1748..>2667
                     /locus_tag="PK218_11020"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HPW99085.1"
                     /translation="MKYFVVTFILAVTFLSCSKEKLSDKAAEKMQDFVVSISNYARSF
                     DSDFIIIPQNGIELAFKNTDPSEGEHPLYMAAVNGFGVEELFYNSNYSPDNERLEILR
                     QLKASKKIMVSEYVIDSTNIADALNRNYSEGFICFPRTSSNYHYLQIPDSVPHSNTAN
                     ITNLSDAQNYLYLISTDNYSSKQEMIHAISATNFDVVLVDLFFNETEFTVSEVNQLKT
                     KANGGKRLVISYISIGSAEKYRYYWKKDWGLHHPLWLKKKYDGYTDEFWVNFWEKEWQ
                     TIIYGNDNSYMKKIITAGFDGAYLDNVEAY"
BASE COUNT          960 a          393 c          508 g          806 t
ORIGIN      
        1 cttaatagac attaggcaaa aaataaatgg tttaataata atgctactta agcgtaaaac
       61 caccaaatgt gaccatgtcg aaaagtttac aaagtattta ataaacaaaa aagaagaaca
      121 aaaaaaagac tcttttgcaa taatttcaac aaattgggat attctaatag acaattcttt
      181 taagagaaat tcaaagggag ttattgatta ttgttgtcat acaactccat atagtggtca
      241 aaatatgcca gcattagttg ctttaactaa gaatttattt actatcaaat tgcttaaact
      301 tcatggttca atgaattggt tacagtgtaa gcgatgtcaa agattatttg taacattttt
      361 tgagaaaata gcgattgatg aatatataaa taaacctatc tgtaaatttt gtaaggaaaa
      421 ttttaaagac gaaaacgcaa atggtggtgc tttattaagc agtcagctta taatgccaac
      481 atttttaaag gatttgaaca atgttcaatt gaaattaatt tggcaaaatg cagggattga
      541 actttctgaa gcttcaagta ttgtatttat gggatattct ttccctacgg ctgactttga
      601 attaagacag ctattagcca gaaatgttag acatagtgct aaaattgaag ttgttttatg
      661 tccatctgat aaaccgacta atgataaaga tacaaactcg cctgaaaata gattcaaatc
      721 attttttggg aaaagagaag ttaatttcta ttatgaagga gttgaggaat atataaaaac
      781 caattacacg taataaagtg ttgaaaaagt gagtcgttca aagattgttt ttcattttaa
      841 tgtttcgtat aatttgacaa aatgtataaa agtttcagta gataaaagat aaattgatta
      901 tggaagcaaa acaggattta aaattggcag ttattataga tgccgacaat gtaccttctg
      961 aaaatgtgaa aggtatgatg gaagagattg caaaatatgg aattcccaca tttaaaagaa
     1021 tttacggcga ttggactaaa ccaaatttag caagttggaa aaaggtgttg ttggaaaatg
     1081 ccattacacc cattcagcag tacgaataca ccacaggcaa aaattccaca gattctgcga
     1141 tgattattga tgctatggac attttgtaca ccggcaaagt ggacggattt tgtattgtgt
     1201 caagcgatag cgattttaca cgattggcga cacgacttag agaatcgggc atgaaagtgt
     1261 tgggatttgg agaaaaaaag acccccaatc cgtttattgt ggcatgcgat aagtttattt
     1321 accttgaaat tttaaagcga ccgatttcgg cagggaaaca gaaaacagaa aattcgcaaa
     1381 aggctaaaca cgagacaatc aagcagatag atttggaaat tatattttta atagcttcga
     1441 cagtaaacga tgttgccgac gaagacggat gggcgttttt gggcgatgtg ggaaatctgt
     1501 taattaagaa aatgcccgat tttgacccgc gaaattatgg ttttgaaaaa ctcactccgt
     1561 tgattaaatc attgaaaagc aattttgata ttgacgaacg aaccacagaa agagcatcaa
     1621 taaaattggt ttatataaag acaaaaaaat agtagtgcaa cactgttgtt tcgttccgat
     1681 gtttggtgta atttgacaag aaaatattcg taaattggaa tttttcacat aaataaggca
     1741 ctgacaaatg aaatactttg tagttacatt cattttggcg gtaacttttt tgagttgctc
     1801 aaaagagaaa ctatcggaca aagctgcaga aaaaatgcaa gattttgtcg ttagtatttc
     1861 caattatgcc agaagtttcg attccgattt tattattatt ccacaaaacg gcattgaact
     1921 tgcctttaag aataccgacc cgagtgaagg agaacatccg ctatatatgg cggctgtaaa
     1981 tggttttgga gttgaagaac tgttttataa cagcaactat tcgcctgaca atgagcgtct
     2041 tgaaatactc agacagctta aggcttcgaa aaaaataatg gtttcggaat atgttatcga
     2101 cagcaccaat attgcagatg ctttaaatcg aaattatagt gaaggattta tttgctttcc
     2161 tcgaacaagc agtaattatc attatttaca aattcccgac agcgttcctc attccaatac
     2221 ggcaaatatt accaatctgt ccgatgctca aaactactta taccttatca gcacagacaa
     2281 ctattcctca aaacaagaga tgattcatgc aatttcggca accaatttcg atgtggtttt
     2341 ggtggattta ttttttaacg aaacagagtt tacagtttcg gaagtcaatc aattaaaaac
     2401 aaaagcaaac ggtggtaaac ggttggttat ttcatacata agtattggct cggcagaaaa
     2461 ataccgctac tattggaaaa aagattgggg gttgcatcat ccgctttggc tcaagaaaaa
     2521 atatgacggc tacactgacg aattttgggt taatttttgg gaaaaagaat ggcagactat
     2581 catttacggg aatgataact cgtatatgaa aaaaattatt actgccggat ttgacggagc
     2641 ttatcttgac aatgtcgaag cctatta
//
LOCUS       DAOOBH010000161         2655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_42710_length_2655_cov_6.113462, whole genome shotgun sequence.
ACCESSION   DAOOBH010000161 DAOOBH010000000
VERSION     DAOOBH010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42710_length_2655_cov_6.113462"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK218_11025"
     CDS             <1..308
                     /locus_tag="PK218_11025"
                     /inference="COORDINATES: protein motif:HMM:NF040945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CCC motif membrane protein"
                     /protein_id="HPW99086.1"
                     /translation="MESKQTLPNSTATLVLGICSLVFGCLFVGLILGIIGLAISGKSK
                     RLYLQNPNDYVGYGALNAGRITSVIGVILGSLSLLYYIIVVVIIGAASLPWLEFLDL"
     gene            631..1467
                     /locus_tag="PK218_11030"
     CDS             631..1467
                     /locus_tag="PK218_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99087.1"
                     /translation="MKKISILPMIALFAIILSSCGGGSKEFTVKNNSVNFAGDLQGYL
                     EVVDGNYTVSKPGSELILTIKLKAIKQLESTKEFDEIRLELTDESGMPISGMSTFTIS
                     KSGWCTTESENTKVDNALKSGSGDFAVQFVYDTYSTGGGSLSRTDALKIASKKAKGFS
                     ITNSKFKEESVSSSESSSSSYSSTTDDEDDEEVVTKKSGSTNWDKVLADYEAYTDKYI
                     KLLKKANAGDASAMTEYMEMLTKAQEFQESLADADDDLTPAQLQKFTKIQMKLANAAA
                     GL"
     gene            1522..2025
                     /locus_tag="PK218_11035"
     CDS             1522..2025
                     /locus_tag="PK218_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99088.1"
                     /translation="MRKLFLFIIILSFTTTIKAQNCSDWVITEYDEVTDITVISMKDR
                     MYIDKDGQRKVSIHVAQKIENGEKETVLTIDIKDGGCISNQNGIIFLFTDGTRLKELN
                     HGADNCVGFAMLNIGIPDYEGNTIKSYLEQKKIKTLRVYTNDSFVQVDFTESNQNKLI
                     ETLKCID"
     gene            2036..>2655
                     /locus_tag="PK218_11040"
     CDS             2036..>2655
                     /locus_tag="PK218_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99089.1"
                     /translation="METSNSVNMETKEKILENLHSQFAENQNHHQGLFIQFLVALLAL
                     FAGFGYVYTHTNPNIQYTQSFVQQVNEVDYFSNQTLLATSVVVLAVLLLLNGILINQG
                     YGFRRDQHLNMVIRKKYLGDDYENVFGKLYNPDKKGFLDYLPNFYIIFFWFILGFQIS
                     VFIAICSKENLVNFADKCFSLTLFITDIILIILSLAFYSKTYCKYR"
BASE COUNT          946 a          390 c          441 g          878 t
ORIGIN      
        1 aaatggaaag taaacaaacg ttaccaaatt caacagcgac tttagtttta gggatttgtt
       61 ctctagtttt cggttgctta tttgtcggac taatacttgg tataatcggc cttgcaatat
      121 ctggaaagag caagagatta tatcttcaaa atccgaatga ttatgtaggc tatggtgctc
      181 taaatgctgg cagaataacc tccgttattg gagtaattct aggttcatta tcattattat
      241 actacataat tgttgtagta ataataggag cagcttcgct tccatggctt gaattcttgg
      301 atttgtagta catatgcttg aaaatttaat acaatggtta caagagcata tgtttccttg
      361 cttttataaa caaacatttg gaattagttg tcctatgtgc ggtttccagc gttcgttaat
      421 agagctgtta aaggggaatt ttatggaaag ttttttacaa ttccctgcat tattacctct
      481 tttattaacc tcatttatct tcattgtatt aaaattactc aaaagtccaa aagcaaaagt
      541 aatagtgcag gttatattac tttttgacct tataattatg attataacct gtttgtacaa
      601 aaacatttat taacaattaa atttttgaaa atgaaaaaaa tatcaatttt accaatgatt
      661 gcattatttg caataattct ttcttcttgt ggtggtggca gcaaagaatt cactgtcaaa
      721 aacaattcag taaattttgc aggagacttg caaggttatt tggaagttgt tgatggtaat
      781 tatactgttt caaaaccagg ctctgaacta atattaacaa ttaagttaaa agcaatcaaa
      841 caattagaaa gtaccaaaga atttgatgaa atacgattag aattaactga tgaaagtgga
      901 atgccgattt ctggcatgag tacatttaca atttctaagt caggttggtg cactactgaa
      961 agcgaaaaca caaaagttga taatgcttta aaaagtggta gtggagactt tgctgttcaa
     1021 tttgtttatg atacatattc aacaggtgga ggttcattaa gtagaactga tgctttaaaa
     1081 attgcttcta aaaaggcaaa aggcttttca attacaaact ccaaatttaa agaagagtct
     1141 gtcagttctt ccgaaagctc gtcaagttct tattcttcca ctactgatga tgaagatgac
     1201 gaagaagttg taacaaaaaa atcaggtagt acaaattggg acaaagtact tgcagattat
     1261 gaagcatata ctgacaaata cattaagttg ctaaaaaaag caaatgctgg tgacgcttca
     1321 gcaatgactg aatatatgga aatgttaaca aaagcccaag aatttcagga aagtttagct
     1381 gatgcagatg atgatttaac acctgcacaa ttgcaaaagt tcactaaaat tcaaatgaaa
     1441 ttagcaaatg ccgctgctgg cttataaact taataacaag ctggggcata gccccagctt
     1501 gttattttaa ctgatttcaa tatgagaaaa ctatttcttt tcatcataat cttatcattc
     1561 actacaacta tcaaagctca aaactgttca gattgggtta taactgaata tgacgaagtt
     1621 actgacatta ctgtaatttc catgaaagat agaatgtata ttgataaaga tgggcaacga
     1681 aaagtatcaa tacatgttgc tcaaaaaatt gaaaatggag aaaaagaaac cgtacttaca
     1741 atcgatataa aagatggcgg ttgtattagc aatcaaaatg gcataatatt tctttttacc
     1801 gatggcacga gattaaagga actaaatcat ggtgcagata attgtgtggg ttttgcaatg
     1861 ctaaatattg gtattccaga ttatgaagga aacacaataa aatcctactt agaacaaaag
     1921 aaaataaaaa cgctcagggt ttatacaaat gattcttttg ttcaagttga ttttactgaa
     1981 tccaatcaaa ataaattaat agaaaccctt aaatgtattg attaactcta aaactatgga
     2041 aacttctaat tcagttaata tggaaacaaa agaaaaaata ttagaaaacc tgcatagcca
     2101 atttgcagag aatcaaaacc accatcaagg cttatttata cagttcttag tagcactttt
     2161 agccctattt gcaggctttg ggtatgttta tacgcataca aatccaaata tacaatatac
     2221 tcaatccttt gttcaacaag taaatgaagt tgactatttc tcgaatcaga cattacttgc
     2281 aacctcagta gttgttttag ctgtattgtt gttactaaat ggaatattaa ttaatcaagg
     2341 ctatggtttt agaagagacc aacatttgaa tatggtaatc cgcaaaaaat acctcggcga
     2401 tgattatgaa aatgtatttg gcaaattata taatcccgat aaaaaagggt ttttggacta
     2461 tttaccaaat ttctatatca tcttcttttg gtttatttta ggttttcaga tttctgtttt
     2521 tatagcaatt tgctctaaag aaaatcttgt caattttgca gacaaatgtt tttcattgac
     2581 cctattcatt acagacataa tattgattat tctttctttg gcattttata gtaagacata
     2641 ttgcaaatac agatt
//
LOCUS       DAOOBH010000162         2593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_44032_length_2593_cov_4.702128, whole genome shotgun sequence.
ACCESSION   DAOOBH010000162 DAOOBH010000000
VERSION     DAOOBH010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44032_length_2593_cov_4.702128"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="PK218_11045"
     CDS             <1..1075
                     /locus_tag="PK218_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW99090.1"
                     /translation="ELLMNTEMESIQKKYMDTINQSANSLMEVINNILDFSKIESGKI
                     DLVIEKHKPEDIANQVIELIQHEANRKNLDLNLIIDKNVPKYIWIDYIRLKQILINLL
                     SNAVKFTEKGSITLEISREKNENEKIAKLKFLVKDTGIGIKAKNQTKIFEAFSQEDAS
                     TTKRFGGTGLGLSISNQLLALMNSKLILNSKLGVGSEFSFDLEVKFTDEEKKNENRTH
                     KTENLKSKSKIITSEPKEIIIAEDNKINMLLAKTLITQILPNATIIEFQNGKELIDEI
                     SKHNPDIILMDIQMPEINGYEATIEIRKNKKLIKTPIIALTAGTVVGEKEKCIEIGMN
                     DYIPKPIDRENLKNIISNWITKS"
     gene            1114..1968
                     /locus_tag="PK218_11050"
     CDS             1114..1968
                     /locus_tag="PK218_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPW99091.1"
                     /translation="MKWERKKSDTFEDRRGMSGGGKALLGGGAIGIVILLLNMFGGET
                     GQTIGNILQQTQGQQQTEQTQNRELTAEEVELGEFSKACFAYNNETWEKVFQENGMQY
                     EVPGMVLFYDGVNTACGSATSASGPFYCPGDKKVYMDLRFFEELKTRFGAQGGDFAIA
                     YVIAHEVGHHLQTILGTSGKVRQLQANKSEAEANKLSVCLELQADFYAGVWAHYNKNL
                     LEEGDIEEALSAAHAVGDDAIQSKMQGHVNQESFTHGTSEQRMEWFMKGYKTGDIKQG
                     DTFSALLK"
     gene            complement(2160..>2593)
                     /locus_tag="PK218_11055"
     CDS             complement(2160..>2593)
                     /locus_tag="PK218_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW99092.1"
                     /translation="EMQRYLSKISGPLLDRIDIHIEVTPVPFEKLTETRPAESSVEIR
                     KRVTKAREIQSQRFEAFENIHYNAQMSTKQIRQYCALDETSLQLLKTAMERLNLSARA
                     YDRILKVSRTIADLEESEIVNSNHIAEAIQYRSLDRDGWLG"
BASE COUNT          994 a          405 c          435 g          759 t
ORIGIN      
        1 cgaactttta atgaataccg aaatggaaag tattcaaaaa aaatacatgg ataccattaa
       61 ccaatccgca aattcgttaa tggaagttat taataatatc ttagatttct caaaaattga
      121 atctggaaaa attgatttag taatcgaaaa acacaaacca gaagacattg ctaatcaagt
      181 tatcgaatta attcaacacg aagcaaaccg aaaaaatttg gaccttaatt taatcattga
      241 caaaaatgtt ccaaaataca tttggataga ctacattcga ttaaagcaaa ttctaatcaa
      301 cctgttgagt aatgctgtaa aatttactga aaaaggaagt attacacttg aaatttctag
      361 agaaaaaaac gaaaatgaaa aaattgcaaa actaaaattc ttagtaaaag atactggaat
      421 tggtattaaa gcaaaaaatc aaaccaaaat ttttgaagct ttttcccaag aagatgcatc
      481 tacaacaaaa cgttttggag gaacaggatt aggtttatcc atttccaatc agcttttagc
      541 attaatgaac agtaaattaa tcctaaacag taaacttggt gtcggtagcg aattttcatt
      601 tgaccttgaa gtcaaattca ctgatgaaga aaagaaaaac gaaaatagaa cacataaaac
      661 cgaaaattta aaatctaaat ctaaaataat aacaagcgaa ccaaaagaga ttattattgc
      721 ggaagataac aaaataaata tgttattagc aaaaacatta attactcaaa tacttccaaa
      781 cgctacaata attgagtttc aaaatggaaa agaactcatc gatgaaattt ctaaacacaa
      841 tcctgacata atattaatgg atattcaaat gccagaaata aatggatatg aagcaacaat
      901 tgaaattaga aaaaataaaa aattaataaa aacacctata attgcattaa ctgctggtac
      961 ggttgttggt gaaaaagaaa aatgcattga aattggaatg aatgattata tacctaaacc
     1021 aattgatcgg gaaaatctga aaaatattat ctcaaattgg attacaaaat cataaattca
     1081 taactttaca taacgactta aatcaattaa actatgaaat gggaaagaaa aaaaagtgac
     1141 acttttgaag atagacgtgg aatgtctggc ggtggaaaag cattacttgg tggtggagca
     1201 attggaatag taattttatt actaaatatg tttggtggcg aaacgggaca aaccattgga
     1261 aatatacttc aacaaactca aggtcaacaa caaactgaac aaactcaaaa tcgtgaactt
     1321 actgctgaag aagttgaatt aggagaattt tcaaaagcat gttttgcata taataatgaa
     1381 acttgggaaa aagtttttca agaaaacgga atgcaatatg aagttcctgg aatggttctt
     1441 ttctatgatg gtgtaaatac ggcttgtgga agtgcaacct cagcttctgg accattttat
     1501 tgccctggcg ataaaaaagt ctatatggat ttacgttttt ttgaagaatt aaaaactcgc
     1561 tttggtgctc aaggaggaga ttttgctatt gcttacgtaa ttgctcacga agttggacat
     1621 catttgcaaa ccattctggg aacatcagga aaagtacgtc aattacaagc caataaaagc
     1681 gaagccgaag ccaataaact atccgtttgt ttagaacttc aagccgattt ttatgctgga
     1741 gtttgggctc attataataa aaatcttttg gaagaaggcg atatcgaaga agctttaagt
     1801 gcggcacacg ctgttggcga cgatgccatt caaagtaaaa tgcaaggtca tgtaaatcaa
     1861 gaatcattca ctcacggaac atctgagcaa cgtatggaat ggtttatgaa aggatataaa
     1921 acaggcgaca tcaaacaagg cgatacattt tcggctttgt taaaataaaa tttctaaaac
     1981 tgcaaaactc acctttaaca atagaagcac ccgaaagcga ttatctctat atcgaaacgt
     2041 ctcaaattcc taaagccgga aaaggattgt ttacagcaat taaaatcata aagatggatt
     2101 ttttagggct acagtaaaga aaacatttac ctatttcaaa ctaaatactg aatattgact
     2161 taccccaacc atccatctct atccaaactt ctgtattgta tggcttcggc aatatgatta
     2221 gaatttacga tttcggattc ttctaagtca gcaatagttc ttgaaacttt taggattcta
     2281 tcataagcac gagctgaaag gttcaaacgc tccatagctg tttttaaaag ttgaagtgat
     2341 gtttcatcca aagcacaata ctgccgaatt tgtttcgtac tcatttgtgc attgtaatgt
     2401 atgttttcaa atgcttcaaa acgttgcgat tgaatttctc tagctttagt aactcgtttt
     2461 cttatttcta cactgctttc tgctggtcgt gtttcggtta gtttttcaaa cggaacaggt
     2521 gttacttcta tgtgaatatc aattcggtct aataatggtc ccgaaatttt actcaaatag
     2581 cgctgcattt ctg
//
LOCUS       DAOOBH010000163         2569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_44570_length_2569_cov_5.985282, whole genome shotgun sequence.
ACCESSION   DAOOBH010000163 DAOOBH010000000
VERSION     DAOOBH010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44570_length_2569_cov_5.985282"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="PK218_11060"
     CDS             <1..1592
                     /locus_tag="PK218_11060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPW99093.1"
                     /translation="KVLTNAKRIDKDGEKPLDQFNRNENGTITDNLIIKGNNLLALHT
                     LKKEFAGKVKLIYIDPPYNTGNDSFQYNDSFNHSTWLTFMQNRLSIAKSLLSESGIIF
                     VHCDNNEQAVLKILLEEIFGKEQFVETITVVNNPRGRDYGGIANMHEFIHIFSKNQFK
                     YKMFRIKDEEKEFPFMDKIGGFEIRELRNRNTAFHIKNRPNLCYPFYVNPQKELDNGF
                     LELSLEPQKGFIEVLPAKSQGIQTVWRWGKEKAAENLNLNIAGKAMQEEGRFMIIEKY
                     RDNSRLARSVWWDKEVNSERGTVHLRELFNNKVFSFPKPETLLARIIEISTNEKDIVL
                     DYHLGSGTTASTAHKMNRQYIGVEQMDYIETVAVERLKKVIGEKVVLKTEEKTQSKLS
                     IASESDAEYYKLEYDKGGVSKSVNWQGGGSFVYLELKKHNQTFIEWIEDAKDTETLLH
                     IWELMKEKSFFKYSIDLKEFDKSISEFKQFTLKMQKEILVKLLDKNQLYVNLSSLNDN
                     DFACTEEEKKVTKDFYEERGK"
     gene            1573..>2569
                     /locus_tag="PK218_11065"
     CDS             1573..>2569
                     /locus_tag="PK218_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HPW99094.1"
                     /translation="MKKEENKMNKNELQRVDNFVIFQTGKGKVNVEVYFHENSLWLTQ
                     KAMAELFEKGRTTITEHLRNIFADNELDENSVCRDFRHTAPDGKEYTTKFYNLRAVTA
                     VGYRANSQRAVVFRRWASEILHEYIVKGFAMDDERLKQMQHFGKDYFDEMLERIREIR
                     LSERRLYQKITDIYTLSADYDSQAKITQQFFSTVQNKLHWAITGKTVAEIIYSEADAK
                     KINMGLKTWKYAPDGKILKSDVSIAKNYLNEEHIKALERIVTSYLDLAENRARNMQIM
                     NMKDWDTFLTMFLELADYPILTHKGKISMLEAKLKAESEFDTYRVIQDKDYISDFD"
BASE COUNT         1026 a          362 c          462 g          719 t
ORIGIN      
        1 caaaagtctt aactaatgcc aaacgtattg acaaagacgg agaaaaaccg cttgaccaat
       61 ttaatagaaa cgaaaacgga actattaccg acaacctaat aattaaagga aataacttgc
      121 ttgctttgca tacactaaaa aaagaatttg caggaaaagt aaaactaatc tatatagacc
      181 cgccgtataa tactgggaat gatagttttc aatataatga tagttttaat cattctactt
      241 ggttgacttt tatgcaaaat agattatcaa tagcaaaatc acttttgtct gaatcaggta
      301 taatttttgt acattgtgat aataatgaac aagctgtttt aaagattcta ttagaggaaa
      361 tttttggtaa agaacaattt gttgaaacaa taactgttgt aaacaatccg cgaggtcgag
      421 attatggcgg aattgcaaac atgcatgaat ttattcacat tttctcaaaa aaccaattta
      481 agtataaaat gtttcgaatt aaagatgaag aaaaagaatt cccatttatg gataaaattg
      541 gtgggtttga aataagagaa ctaagaaaca ggaatacagc atttcatatt aaaaatcgtc
      601 caaacctttg ctatccattt tatgtaaatc cacagaaaga gttagacaat ggctttcttg
      661 aattatcact tgaaccacaa aaaggcttca tagaagtatt gccagcaaaa tcacaaggaa
      721 ttcaaactgt ctggcgatgg ggtaaagaaa aagcagccga aaatctaaat ctaaatattg
      781 ctggtaaagc aatgcaggaa gaaggcagat ttatgataat tgaaaaatat cgtgataatt
      841 caagattagc acgttctgtt tggtgggata aagaagttaa ctctgaacgt ggaacagtcc
      901 atttacgtga actatttaat aataaagtct ttagttttcc aaaaccagaa acattattag
      961 caagaataat agagatatca actaacgaaa aagacattgt tttagattat catttaggca
     1021 gtggaacaac agcatcaaca gctcataaaa tgaaccgtca atacattggt gtagaacaaa
     1081 tggattacat tgaaacggtt gcagtggagc gacttaaaaa agtgataggc gaaaaagtag
     1141 ttttaaaaac agaagaaaaa acacaatcaa aattgtccat agcaagcgaa tctgatgctg
     1201 agtattacaa attagaatac gataaaggtg gtgtttcaaa atccgttaat tggcaaggcg
     1261 gtggttcatt tgtctatcta gaacttaaaa agcacaatca aacatttatt gagtggattg
     1321 aagacgctaa agatacagaa acgcttttac atatttggga gttgatgaaa gaaaaatctt
     1381 tctttaagta tagtattgat ttaaaagagt ttgataagtc catttcggag tttaaacaat
     1441 ttacactaaa aatgcaaaag gaaatactgg taaaattatt agacaaaaat caattatatg
     1501 taaatctttc ttcgctaaac gataatgatt ttgcctgcac tgaagaagaa aagaaagtaa
     1561 ccaaagattt ttatgaagaa agaggaaaat aaaatgaata aaaatgagtt gcaacgtgtt
     1621 gataactttg ttattttcca aactggaaaa ggaaaagtga atgtagaagt atattttcat
     1681 gaaaacagtt tgtggcttac ccaaaaagca atggctgaat tatttgaaaa aggcagaaca
     1741 accattacag aacatttgcg taacatattt gccgacaatg agttagacga aaattcagta
     1801 tgtcgggatt tccgacacac tgcccctgac ggaaaagaat atactacaaa attttataat
     1861 ttgcgtgctg ttacagctgt gggttatagg gcaaactcac aacgtgcagt tgtttttaga
     1921 cgatgggcaa gcgaaatttt acacgaatat atcgtcaaag gctttgcaat ggacgatgaa
     1981 agattgaaac aaatgcaaca ttttggcaaa gattacttcg acgagatgtt agagcgaatc
     2041 cgtgaaatac gcctcagtga aagaagactt taccaaaaaa taacagatat ttacactcta
     2101 tcagctgatt atgacagtca agccaaaatt acacaacaat ttttttcaac tgttcaaaac
     2161 aagttgcatt gggctattac aggtaaaacc gtagctgaaa taatttacag tgaagctgat
     2221 gcaaaaaaaa taaatatggg gctgaaaact tggaaatatg ctccagatgg aaaaatacta
     2281 aaaagcgatg tctcaatagc aaaaaactat ctaaatgaag aacatattaa agctttagaa
     2341 agaattgtaa cttcctattt agatttagca gaaaatcgtg caagaaatat gcaaatcatg
     2401 aacatgaaag actgggacac ttttttaact atgtttttag aactggctga ttacccaatt
     2461 ttaacacata aaggaaaaat atcaatgctc gaagcaaagt taaaagctga aagtgaattt
     2521 gatacatata gggtaattca agataaagat tatatttcag attttgaca
//
LOCUS       DAOOBH010000164         2558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_44821_length_2558_cov_5.405114, whole genome shotgun sequence.
ACCESSION   DAOOBH010000164 DAOOBH010000000
VERSION     DAOOBH010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44821_length_2558_cov_5.405114"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1902
                     /locus_tag="PK218_11070"
     CDS             1..1902
                     /locus_tag="PK218_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HPW99095.1"
                     /translation="MISNIAHFLKEHEPFTYLTSDELNSIASNIGIINLEKNKTLFQM
                     EDALHDTFYVVASGVINLTFIADGEEIILNKCYAGDVFGLRPFFAKNNYMMTAKSRED
                     SIVYAIPIAIFKPFVSQNSSILDFLLENFATNTRSSLDQENGTTASDGIYSNKTGEIQ
                     YFQSLSYNKTPLKINQKQLAKDVAQLMSDNLIDSAIIVDDNHFPIGIATDTDFRSKIA
                     NGRFPLNSEIYKIMSSPVFTVPENISVAEAQLLLLKYNVSHLCVTKDGSDKSSISGII
                     SEHDIVVAQSNNPGVLIKQIKKALTPKELKQVREKLTELVQSSISKNIPIPHIFNITG
                     EINFAIIKRAIEIAILDLGSPPARFCWISIGSQARKEQLLLTDQDSILVFEDVAQEKY
                     RDVKDYFVKLAKRATTILEKTGFYYCPNNHMASNLIWCKSLTEWIKQYDNWMKTPGEK
                     SNEIGSIFFDIEMVIGENKIEDTIVETIISNSKNNTLFFDYLGNNALHKEPSLTFFKK
                     FNVEEEGQHKGKFDIKNKAIMPLVDGARLFALSMQLKGVNNTYLRFKQLAIVDPKFAE
                     IYLNCAESFLILTNFRTLEGLKNDTSGEFINLEELTKIDKEKLKNALLPMKELEDLIK
                     DKFQLTQFS"
     gene            1902..2501
                     /locus_tag="PK218_11075"
     CDS             1902..2501
                     /locus_tag="PK218_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPW99096.1"
                     /translation="MFDWIKNINKEHPEFWKNYLEKFESKTKTKRYVILNTETSGLNP
                     KKDVILSFGAIGIENDIIKVNDALELVILQYKYLHDNGLSNEFIIESSLPKFAEPTGI
                     EELINYIGNATLVGHRIHFDIEMINEALDRIDCGKIKNEALDIEIMHQKLYEEINKPY
                     SLDDLVKTYKIPVSEKNFALDDAYSMALLFLKLKSRLGF"
BASE COUNT          976 a          356 c          399 g          827 t
ORIGIN      
        1 attatttcaa atattgctca ctttttaaaa gaacacgaac cttttacata tttaactagt
       61 gatgaattaa attcaattgc ttcaaatatt ggcattataa atcttgaaaa aaataaaact
      121 ttatttcaaa tggaagatgc tttgcatgac acgttttatg ttgttgcttc tggagtaata
      181 aatttaacct ttattgctga tggtgaagag attattttaa acaaatgcta cgctggtgat
      241 gtttttggac taaggccgtt ttttgccaaa aacaattata tgatgacagc taaatctaga
      301 gaggattcaa ttgtttatgc aatacctatt gctatattca aaccttttgt ttctcaaaat
      361 tcgagtattc ttgattttct tttagaaaat tttgccacaa atacacgaag ttcacttgat
      421 caagaaaatg gaacaacagc atcggatggt atttatagta acaaaacggg agaaattcaa
      481 tactttcaat cattaagtta taataaaact ccattaaaaa tcaaccaaaa acaactcgct
      541 aaagatgttg ctcaacttat gtctgataat ttgattgaca gtgcaataat agttgacgat
      601 aaccactttc ctattggaat tgctacagat actgacttta gaagtaaaat tgcaaatggt
      661 agatttccac taaattctga aatatacaaa atcatgtctt ctccagtatt tactgttcca
      721 gaaaacattt ctgtagccga agcacaactt ttgcttctaa aatacaatgt tagtcatctt
      781 tgtgtgacta aagacggctc tgataaatca tcaatttcag gaatcatctc cgaacatgac
      841 attgtggtag cacaatcaaa taatccaggc gtgttaataa aacaaattaa aaaagcatta
      901 actcctaaag aattaaagca agttagagaa aaattgaccg aattggtgca atcctctatc
      961 tcaaagaata tcccaattcc tcatatattt aacattacag gcgaaataaa ttttgctatt
     1021 attaaacgtg caatcgaaat cgccattctc gatttaggat cacctccagc tagattttgt
     1081 tggattagta ttggtagtca agctcgtaaa gaacaactgc tattaaccga tcaagatagc
     1141 attttagttt ttgaagatgt tgcgcaagaa aagtaccgtg atgttaaaga ttattttgta
     1201 aaactagcaa aaagagccac aacaatttta gaaaaaacag gtttttatta ttgcccaaat
     1261 aatcacatgg caagtaatct tatttggtgt aaatcgttga cagaatggat taaacaatat
     1321 gacaattgga tgaaaacacc aggagaaaaa tcaaatgaaa ttggaagcat attttttgat
     1381 attgaaatgg ttattggtga aaacaaaata gaagatacta tagtggaaac aattattagt
     1441 aattctaaaa acaatacttt gttttttgat tatttgggaa ataatgcgct gcacaaagag
     1501 ccatcattaa cttttttcaa aaaatttaat gtcgaagaag aaggtcaaca taaaggtaaa
     1561 tttgacataa aaaacaaagc cataatgcca ttagttgatg gtgctcgatt atttgcttta
     1621 agtatgcagt taaaaggtgt aaataataca tacttgcgtt ttaaacaatt agctattgtt
     1681 gatccgaaat ttgctgaaat ctatttaaat tgcgccgaat cttttttaat tttaacaaat
     1741 tttagaacat tagaaggttt aaagaatgac acttcgggtg aatttattaa tttggaagaa
     1801 ctcaccaaaa ttgataaaga aaaattaaaa aatgctttac tgccaatgaa agaattggaa
     1861 gatttaataa aagataaatt tcaactaact cagttttcct aatgtttgat tggataaaaa
     1921 atattaataa agaacatcct gaattttgga aaaactatct tgaaaaattt gaatcgaaaa
     1981 caaaaacaaa aagatatgtt attctgaata cagaaactag tggtttgaat cccaaaaaag
     2041 atgttatatt atcttttggt gctattggta ttgaaaatga tattattaaa gtaaatgatg
     2101 ctttggaatt ggttatttta caatacaaat acttacatga caatggatta tcaaacgaat
     2161 ttatcatcga aagtagtttg ccgaaatttg cagaaccaac aggtatagaa gaattgataa
     2221 attatattgg caatgcaaca ttggttggac atagaattca ttttgatatt gaaatgatta
     2281 acgaggcttt agatagaata gattgtggca aaattaaaaa cgaagcatta gatattgaaa
     2341 ttatgcacca aaagctttat gaagaaatca ataaacctta ttcgttagac gatttagtaa
     2401 aaacatacaa aattcccgtt tccgagaaaa attttgcttt agacgatgcc tattctatgg
     2461 cattgctttt tttgaagtta aaatctagac tcggatttta atcaaataaa aacccattca
     2521 cgcggaatgg gttcttaaag atctgtcaaa atcaaaca
//
LOCUS       DAOOBH010000165         2541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_45193_length_2541_cov_7.459372, whole genome shotgun sequence.
ACCESSION   DAOOBH010000165 DAOOBH010000000
VERSION     DAOOBH010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45193_length_2541_cov_7.459372"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK218_11080"
     CDS             complement(<1..222)
                     /locus_tag="PK218_11080"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HPW99097.1"
                     /translation="MALTQLQIQQVETVLRNSLRYKFQNYKPEPASMPFHTRLLGKDR
                     MALFSFIHSLNTNFGTSIFEPVALAIASMN"
     gene            complement(188..1921)
                     /locus_tag="PK218_11085"
     CDS             complement(188..1921)
                     /locus_tag="PK218_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HPW99098.1"
                     /translation="MAQKFDIGLNAKINEFAKKFSIARTATNDSDVFEDFGNYVIASN
                     LLEEEIENIKSVSTNKAQGIDGVVIIINNILVSEEADLEKFGPTESIKFKIGFIQSTI
                     ENSFNEQKLSAFTDEVVKFLIGEIAIEPYSTIYKKLLDETGNFIDRIEETPHVSLFFL
                     SARTSHNIDDDKIKSEENKITSRNEIKNKCILDKFFVLQNEDIKNEYEKISKFHTVQL
                     KLENSITLSPVKNVEISLLATIKFLELKKLILTSDNNLKEKLFVENVRNYIGNTPVNT
                     DIKKTLDDTSKRSYFPFLNNGLAIICDKIERHPVKDKVFLLTFPRIINGCQTTNELFK
                     KYKDSSDEKSIDEIEVVAKIISTEDNDLKKMIIYAANNQNSIDRDLQSLNEFHEKIET
                     YFLGKEEKNFHLYFERLRGQHANIIPPYSKINIETLARVFISVFLKKPHEMKSNAISV
                     IEKFQKEGKIFNSSHDKPNQYYYCSVLWYWFNYLLINRNFALNTKSMDMHVLMACDIL
                     LEKKNYSSIDEKINYLGETSNAMSIFSETVTLLNEKSYLFERRGFYSNPKTERLINEI
                     NGINTITNTAS"
     gene            complement(1926..>2541)
                     /locus_tag="PK218_11090"
     CDS             complement(1926..>2541)
                     /locus_tag="PK218_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009422404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPW99099.1"
                     /translation="EPVTTTYYCAKHGKICKPLFSILKWWETYSKDTVKRLAQFDKLR
                     TQTFQTCLTGDSRTIDIFAELKKKNPAFGELAEKQKIKGIFSSPPYVGLIDYHEQHAY
                     AYDLFGFERRDELEIGPLFKGQGKEAKQSYIQGISDVLNNCKKYLADDYNIFLVANDK
                     YNMYPTIAENAGMKIVKQFKRPVLNRTEKDKGAYSEIIFHLKCK"
BASE COUNT          770 a          401 c          360 g         1010 t
ORIGIN      
        1 attcattgaa gctattgcta aagcaacagg ttcaaaaatg cttgttccga aatttgtgtt
       61 taacgaatgt atgaacgaaa acaaagccat tcggtctttc cctaagagcc gtgtgtggaa
      121 aggcattgaa gcaggttcag gcttataatt ctgaaattta tagcgtaagc tgtttcttaa
      181 aactgtttca acttgctgta tttgtaattg tgttaatgcc atttatttcg tttattaatc
      241 gttcagtttt gggatttgaa taaaaacctc ttctttcaaa taagtaactt ttttcattta
      301 aaagagttac tgtttctgaa aagatagaca ttgcattgct ggtttctcct aaataattta
      361 ttttttcatc aatggatgaa tagttttttt tctctaaaag aatatcacaa gccatgagta
      421 cgtgcatatc cattgatttc gtatttagtg cgaaatttct attgattaat aaataattaa
      481 accaatacca caatacgcta caatagtaat attgatttgg tttgtcatga gagctattaa
      541 aaattttgcc ttctttttga aatttctcta tcactgaaat agcattgctt ttcatttcat
      601 gaggtttttt cagaaaaacg gaaatgaata ccctagctaa tgtttcaata tttattttgc
      661 tatatggtgg aatgatattt gcatgttgtc ctcttagtct ttcaaaatat aaatgaaaat
      721 ttttttcttc tttcccaaga aaataagttt caattttttc atgaaattca ttaagtgatt
      781 gtaggtctct atcaatggaa ttctgattat ttgcagcata aataatcatt ttctttagat
      841 cattgtcctc agttgaaatt attttagcaa caacttcaat ttcatcaatg ctcttttcat
      901 ctgatgaatc cttatatttt ttgaatagtt cattagtagt ttggcatccg tttataattc
      961 tcggaaatgt taataaaaac actttatctt ttacagggtg tcgctctatt ttatcgcaga
     1021 taattgccaa tccattattt aaaaaaggga aataacttct tttagaagta tcatccaaag
     1081 tcttttttat atcagtattt actggagtgt ttccaatgta atttcgtaca ttttcaacaa
     1141 ataatttttc tttcaaattg ttatcagaag tcaaaattaa tttttttaat tctagaaatt
     1201 ttattgttgc taaaagactt atctctacat ttttcacagg cgaaagtgta atgctatttt
     1261 ctaattttag ttgtactgta tgaaacttgg aaattttttc atattcgttt tttatatctt
     1321 cattttgcaa aacaaaaaac ttgtctaaaa tgcatttatt ttttatttca tttcttgagg
     1381 taatcttatt ttcctcagat tttattttat catcgtcaat attatgtgaa gtcctagctg
     1441 agaggaaaaa taaagataca tgtggcgttt cttctattct gtctatgaaa tttcctgttt
     1501 cgtccagcaa ctttttatat atagttgaat aaggttcaat agcaatttca cctataagaa
     1561 actttaccac ttcatccgta aaagcactta atttttgttc attaaaacta ttttcaattg
     1621 tagattgaat aaaaccgatt ttaaatttaa ttgattccgt tggaccaaat ttttctaaat
     1681 cggcttcttc agaaaccaat atgttattaa taataataac aacaccatcg ataccttgtg
     1741 ctttgtttgt tgaaacggat ttaatatttt ctatttcttc ctctaacaaa ttagaagcta
     1801 tcacataatt gccaaaatct tcaaaaacat cactatcatt tgttgcagtt cttgctatgc
     1861 taaacttttt tgcaaactca ttaatcttag catttagtcc tatatcaaat ttttgtgcca
     1921 taatgttatt tacactttaa atgaaaaatt atttccgaat atgctccttt gtccttttca
     1981 gttcggttca aaacaggtcg tttaaattgt ttcacaattt tcattcctgc attttccgca
     2041 atcgtaggat acatattgta tttgtcattt gctacaagaa aaatattgta gtcgtctgca
     2101 aggtattttt tacaattgtt caaaacgtct gaaattcctt gtatgtagct ttgttttgct
     2161 tctttacctt gtcctttgaa aagcggacca atttctaatt cgtctctacg ttcaaatcca
     2221 aacaaatcgt aagcataggc atgctgttcg tggtaatcaa ttaaaccaac gtaaggcggc
     2281 gatgaaaaaa tacctttgat tttttgcttt tctgccaatt ctccaaaggc aggatttttc
     2341 tttttgagtt cagcaaaaat atcaattgtt ctgctgtcgc ccgtcaaaca ggtctgaaag
     2401 gtttgagttc ttaatttgtc aaattgagca aggcgtttta cagtgtcttt actgtatgtt
     2461 tcccaccatt taagaatgga aaaaagtggt ttgcatattt ttccgtgctt tgcacagtag
     2521 taagtcgtag taacaggttc a
//
LOCUS       DAOOBH010000166         2473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_46856_length_2473_cov_6.093466, whole genome shotgun sequence.
ACCESSION   DAOOBH010000166 DAOOBH010000000
VERSION     DAOOBH010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46856_length_2473_cov_6.093466"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PK218_11095"
     CDS             <1..446
                     /locus_tag="PK218_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW99100.1"
                     /translation="TVNPILKYLIDSEFYSKIQKQYIDNISIKIIENDSIINQINSFL
                     NTFSNTVNGSQKSDKLVYYNENSQLNDVIKTKDDLVREQGIHRIELINLNKIIKNSAE
                     VLNIKYTETINGKLKFILPLILVALFILVGLIKAYYKRQMTKLNS"
     gene            449..1312
                     /gene="rfbA"
                     /locus_tag="PK218_11100"
     CDS             449..1312
                     /gene="rfbA"
                     /locus_tag="PK218_11100"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPW99101.1"
                     /translation="MKGIILAGGSGTRLHPLTLAVSKQLMPIYDKPMIYYPLSTLMWA
                     GINEILIISTPHDLPLFQQLLGDGKKFGCRFEYAVQENPNGLAEAFIIGKEFVANDKV
                     ALILGDNIFYGTGLAELLQANNNPDGGIIYAYHVHDPERYGVVDFDKQGNVLSIEEKP
                     EKPKSNYAVPGIYFYDNSVIEVAANIKPSHRGELEITDVNKEYLKRGNLKVSILDRGT
                     AWLDTGTFQSLMQAGQFVQVIEERQGLKIGAIEEAAYKMGFIDKNQLKILAEPLLKSG
                     YGVHLMALLEE"
     gene            1372..1923
                     /gene="rfbC"
                     /locus_tag="PK218_11105"
     CDS             1372..1923
                     /gene="rfbC"
                     /locus_tag="PK218_11105"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPW99102.1"
                     /translation="MIFTETKLKGCFIIEPKVILDERGYFMESFNEETFQKGIGQTVH
                     FVQDNQSFSTKGVLRGLHYQTGEHAQAKLVRVLQGEVLDVAVDIRPNSKTFGQYEAIV
                     LSSENQKQFFVPRGFAHGFLVLSETAVFFYKCDNFYNKESEGGIDFNDKTIGIDWQFP
                     LENLIISEKDKVQPNLENAKKAW"
     gene            1917..>2473
                     /locus_tag="PK218_11110"
     CDS             1917..>2473
                     /locus_tag="PK218_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW99103.1"
                     /translation="MVVLVTGSSGQLGQSLQFIAPNYSEIEFVFCDSATINITDLNSI
                     KQAFKQFKPNFCINAAAYTAVDKAESEPEKAFDINVNGSKNLAIVSKEFDTVLLHIST
                     DFVFDGTLSSRAQSKGYLETDIPNPTGVYGQTKLEGEIAIQNTWKKHFIIRTSWVYSQ
                     FGNNFMKTMLRLASERDTISVVNDQ"
BASE COUNT          916 a          351 c          429 g          777 t
ORIGIN      
        1 aaactgtaaa tccaatttta aaatacttaa ttgattctga gttttatagt aaaatccaaa
       61 agcagtatat tgacaacatt agcattaaaa taattgaaaa tgattctatt atcaatcaga
      121 ttaatagttt tttaaataca ttttcaaata ctgttaatgg ctctcaaaaa agtgataaat
      181 tggtttatta caatgaaaat tcacaattaa atgatgtaat caaaacaaaa gatgatttgg
      241 ttagagagca aggaattcat agaattgaat taattaattt aaataaaatt attaaaaata
      301 gtgccgaagt acttaatatc aaatatacag aaactataaa tggtaaatta aaatttattc
      361 taccattaat tttagtggca ttattcattt tagtaggact aatcaaggct tactataaaa
      421 ggcaaatgac taaattaaac tcttaaacat gaaaggaatt attctagctg gcggttcagg
      481 aacaagattg cacccattaa cattagcagt aagcaagcaa ttaatgccta tttatgacaa
      541 accgatgatt tattatccgt tgtcaacttt aatgtgggca ggaatcaatg aaattttaat
      601 tatctcaaca cctcacgatt taccactttt ccaacaatta ttaggtgatg ggaaaaagtt
      661 tgggtgccgt tttgaatatg ctgttcaaga aaaccctaac ggattggcgg aagcatttat
      721 tattgggaaa gagtttgtag ctaatgataa agttgccttg attcttggtg ataatatatt
      781 ttacggaaca ggtttagcgg agttattaca agcaaacaac aatccagatg gcggaataat
      841 ttatgcttat catgttcacg atcccgaacg atatggtgtg gttgattttg ataaacaagg
      901 aaacgttttg tccattgaag agaaaccaga aaaaccaaaa tctaattacg cagtacctgg
      961 aatttatttt tatgataatt cggttattga agttgctgcc aacataaaac caagtcatcg
     1021 aggagagctt gaaattaccg atgttaacaa ggaatatcta aaaagaggaa atctaaaagt
     1081 ttctattttg gatagaggaa cagcttggct tgatacagga acattccaat cgttgatgca
     1141 agcaggtcaa tttgttcaag ttatagaaga acgtcaaggt ttaaaaattg gtgctatcga
     1201 agaagctgct tataaaatgg ggtttataga taaaaatcaa ttaaaaatat tagcagaacc
     1261 attactaaaa agtggttatg gtgtccattt gatggcattg ttagaagaat aataagggtt
     1321 taaccacaat gttattgaaa ttgattttta acattgatat tgatattgag catgattttt
     1381 acagaaacca aacttaaagg ttgttttatc attgaaccca aagttatttt ggacgaacga
     1441 ggttatttta tggaaagttt caatgaagaa acattccaaa aaggaatagg acaaacggtt
     1501 catttcgttc aagataatca atctttttct actaaaggcg ttttacgcgg tttacattat
     1561 caaactggtg aacatgctca agccaaatta gtgcgagtat tgcaaggaga agtgcttgat
     1621 gtagctgtcg atattcgtcc taattctaaa acatttggtc aatatgaagc tatagtttta
     1681 tctagtgaaa atcaaaaaca attttttgtt ccaagaggat ttgctcacgg atttttagta
     1741 ttgagcgaaa ccgctgtttt tttctacaaa tgcgacaatt tttacaataa agaatccgaa
     1801 ggaggaattg actttaatga taaaaccatt ggaattgatt ggcaatttcc attagaaaat
     1861 ttaatcattt ccgaaaaaga taaagtacaa cctaaccttg aaaatgccaa aaaagcatgg
     1921 tagttttagt aacaggaagt tcaggacaat taggacaaag tttgcaattt attgctccaa
     1981 attattccga aattgaattt gttttttgcg attcggctac aataaatata acagatttaa
     2041 acagcatcaa acaagctttt aaacaattta aaccaaactt ttgtataaat gcagcagcat
     2101 acactgcggt ggataaagct gaaagcgaac cagaaaaagc atttgatatt aacgtaaatg
     2161 gatcaaaaaa cttagcaatt gtctccaaag aatttgacac agtcctatta catatttcta
     2221 ctgattttgt ttttgacggt actttgtcat ctcgagcgca gtcgaaaggc tatctagaaa
     2281 cggatattcc taacccaact ggagtttatg gacaaacgaa actagaaggt gaaattgcaa
     2341 ttcaaaatac ttggaaaaaa cattttatca tccgtacatc ttgggtctat tctcaatttg
     2401 gaaataattt catgaaaacc atgttgcgat tggcatccga aagagatact atttcagtag
     2461 taaacgacca aat
//
LOCUS       DAOOBH010000167         2190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium sp. isolate AMR_MDS_4049
            NODE_54899_length_2190_cov_8.102108, whole genome shotgun sequence.
ACCESSION   DAOOBH010000167 DAOOBH010000000
VERSION     DAOOBH010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866020
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium sp. (wastewater metagenome)
  ORGANISM  Flavobacterium sp.
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:11:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,223
            CDSs (total)                      :: 2,196
            Genes (coding)                    :: 2,180
            CDSs (with protein)               :: 2,180
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 7 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Flavobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54899_length_2190_cov_8.102108"
                     /isolate="AMR_MDS_4049"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:239"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..>2190)
                     /locus_tag="PK218_11115"
     CDS             complement(20..>2190)
                     /locus_tag="PK218_11115"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPW99104.1"
                     /translation="KCIDEYGSDLHPNIVFENTGIDQSVFDKKGDQRKCLYIENLDDA
                     LESVKVEYSINDLSLMLQYLHKNGIILYEEDLKKIFSIYKRQNEIDYFIHKDAEGFLK
                     EQFDIYVYNWLFNDLDTDFDAATVKRIQNIKKIAHKVIEYIARFEDELKAIWEKPKFV
                     RNCNYVLTLDRLSEHTDLIEKLIKSPGWEKQVEEWQKLSEAWIDEKTNEVLKKAFDDF
                     IGANLNAEKIIEDNKLSSHYQFLPINTIHFEDLKFDILKSIPDLDNSIDGILIKSDNY
                     TALNTIKVKYKNKIDFTYIDPPFNLGHNADFKYKVNYKDSTWLTLLENRITLNYDLLS
                     DNGVFLLRNDYNGNYLTRQLLNSIFQVVNFRNELQVNRIFKNKTDNKIKSLPFSLDTV
                     HVYAKTEAFYYKNPFVERTEERAAFWRHMNDSAGQGSAKLFFGLSLEPPIGKHWKYSQ
                     ENIDNKIKNKLLKLFCKNCGYTHSEGDVYNCPECGSFDWNPKYLVKASNTKILGGDWT
                     DIGVNSNYLELHNNWNDIPGYSNQTAFSTENSLALLERAVKICTNEGDLVLDYFLGSG
                     STIVAAHRLNRKWIGIEIDKQYDNVAFPRMYEILSKHGGVFKYYELEQYEEVLAKAQY
                     QWQGKKGENQVEQYSFLQDQKLLEAIEIDYEKKNAKVVFEKLYPDVDIAETLSNLSGK
                     HIKQLFEDKVVFEDGSEVIYDEMTFEKYPWIKPLIWWNSK"
BASE COUNT          656 a          382 c          303 g          849 t
ORIGIN      
        1 ttagccatag tttgtcagtt tatttgctat tccaccaaat caatggttta atccaaggat
       61 atttttcaaa agtcatttcg tcataaatta cttcactgcc atcttcaaac actaccttat
      121 cttcaaatag ttgcttgatg tgcttgcctg aaaggttaga aagcgtttcg gcaatgtcca
      181 catctggata gagcttttca aacactactt tggcattttt cttttcgtaa tcaatctcaa
      241 tagcttctaa taacttttgg tcttgtaaga agctatattg ttctacctga ttttcccctt
      301 ttttgccttg ccattggtat tgagctttag caagcacttc ttcgtattgt tcgagctcgt
      361 agtatttaaa aacaccacca tgttttgata ggatttcgta cattcttgga aaagcaacat
      421 tgtcatattg tttatctatt tcgattccaa tccattttct atttaatcta tgagctgcaa
      481 ctattgtgga accgctgcct aaaaaataat ctaacactaa atcaccttcg ttagtacaaa
      541 tttttacagc tctttctaat agagccaaag aattttcagt tgaaaatgca gtttggtttg
      601 aataacctgg tatgtcattc caattattat gtaattctaa ataattggaa ttaactccaa
      661 tatctgtcca atcaccgccc aaaattttgg tgtttgaagc ttttactaaa tattttggat
      721 tccaatcaaa cgaaccacat tctggacaat tataaacatc gccttcacta tgagtgtatc
      781 cgcaattttt acagaataat tttagtaatt tgtttttaat tttattgtca atattttctt
      841 gagaatattt ccagtgtttt ccaattggag gttcgagaga taatccaaag aaaagttttg
      901 cagatccctg tcctgcacta tcattcatat gtctccaaaa agcggctctt tcttctgttc
      961 tttcaacaaa agggtttttg taataaaaag cttcagtttt agcgtataca tggacagtat
     1021 ccaaactaaa tggtaaagat tttattttgt tatctgtttt gttcttaaaa atcctattta
     1081 cttgtaattc atttctaaaa tttacaacct gaaaaattga attcaacaat tgtctagtta
     1141 gatagttgcc attataatcg ttccttaaaa gaaacactcc attatcagat agtaaatcat
     1201 aatttagagt tattctgttt tccaataatg tgagccatgt tgaatctttg taattaactt
     1261 tatatttaaa atctgcatta tgccctaaat tgaaaggtgg gtcgatatat gtgaagtcta
     1321 ttttattttt atactttact ttaatagtat ttaaggcagt atagttatct gattttatta
     1381 aaataccatc aatagaattg tctaaatcgg gtatagattt taatatatca aatttcaagt
     1441 cttcaaaatg aattgtatta atcgggagga attgataatg tgatgataat ttgttgtctt
     1501 caattatttt ttctgcattt aaattagcac cgataaagtc gtcaaatgct ttttttaaaa
     1561 cttcattagt tttttcatct atccatgctt cagataattt ttgccattct tcaacttgct
     1621 tttcccaacc tgggctttta atcaatttct ctatcaggtc ggtatgttca cttaatctat
     1681 caagtgtcaa tacataatta cagtttctca caaactttgg tttctcccaa atcgctttta
     1741 gttcatcttc aaatcgggca atgtattcaa taactttgtg tgcaattttt tttatatttt
     1801 gtattcgctt cacggtcgct gcatcaaaat cagtatctaa atcattgaat agccaattgt
     1861 aaacgtaaat atcaaactgc tctttcaaaa acccttcggc atccttgtgt atgaaatagt
     1921 ctatttcgtt ttggcgtttg taaatgctga agattttctt caaatcttct tcatacaaaa
     1981 tgattccatt tttgtgcagg tattgaagca ttaacgataa gtcattaata ctgtactcaa
     2041 ccttaacaga ttcaagtgca tcatctaaat tttcaatgta taggcatttc ctttggtcgc
     2101 cttttttgtc aaataccgat tggtcaatac ctgtgttctc aaaaacaata tttgggtgta
     2161 aatcagaacc atattcatca atacacttgt
//
