LOCUS       DAOOBX010000001        11960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_6831_length_11960_cov_4.721714, whole genome shotgun sequence.
ACCESSION   DAOOBX010000001 DAOOBX010000000
VERSION     DAOOBX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6831_length_11960_cov_4.721714"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..>11960
                     /locus_tag="PLY30_00005"
     CDS             10..>11960
                     /locus_tag="PLY30_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64511.1"
                     /translation="MTVSTYEVTGDGKVLRDVMTWKLGADGRFGTADDVLTFVESYDP
                     VTKVRFQQNYDDQGRVILYRNLGTGDELRYTFDDAAGKVTLTSSGNYFKQVYKFDEAH
                     PGDLSFARLVEEERDGVKRIWDEDEQLVSETDLATGIETKVASGHFVTSVTDKDGVTT
                     FFRDGVILSVIKRDGTVLRTYRHIVDRGTGYLTDVDFDGKSSLAKVSICLDGAGAGCS
                     NYRVIYENGVMLEYEMKGDRPVLVWVGDPLGTEFTQSTDAATGELTIRYNDGRKSVYS
                     QVAGFYEIRRSYSLRGELETYCYFGSDMDGACASVDIDAAKAQMRAAVGALPGTGIDT
                     GTAKFEVPELLAGDPGLDFSVKADGTLVFYAKRSAEEWQDIPGSLFTIGEFGRVTVSQ
                     YSGTDLQQTIELRGDLTLANVTKYDEDSRVAASYTYNAGGTKLLTRSVYTYVSTDKED
                     KDYRLLKTVETYNVGDLNLRDIAKLPEDAGTLQSRSHYLARHAASKAAEGESSAVTES
                     LPGRFDYLGLGLVDYTENYLKPLEAGGAPLFQSIVRHEYDVFRLVRSVTYKNLTGTSV
                     CGDGFSGCEMTSMDEFTYYAGTDDIDFTTRWSITNNSLDHKVKQSVFRTEDVADEVTV
                     THDFGADFVEDSEDDEFTATISREVGDDLDGNASMENWGSEVYTYTGISDYSFTVKGG
                     YKRAYAGGLFTGLKSHSIQYDVYGDGTLTRSKNFEFIQGIETGKVTFSESRRMTGAMR
                     GYEGTLGDTLLGDLSGDQLEQFLGQDFTFTRSWQFGFDRPEEDRIFRSESYSVTSRNE
                     KMTRTVSRDVITDLLTYTHQEEISDSAVRSELVGLGAIAVTSGESLSMSWSYAGAFNS
                     DLRFLLDRKISLDVYGEGSLIRSKNFVVENGAETGEINFSETKNIPHNEMSYDGTHTV
                     SELLLGVDAGKVEWSDPVSRTQTWTHGFSTQVSDRYFEGESYSLTSVDGKITRSANWD
                     AIKGEVHYNYVEQVKVPYDTYTVGGVHVGETLIGDVKSSSNWNPKTILYMTSSRDSFY
                     VSNRFIDSESYQITSRDRMETRTLSIDRYHPDGPKVSYSGSVRVTRETVTALIADEKN
                     TILPGDAKAFLDVFSDPEFANNPFYLVTGSTDGFNDPGRVITSKMCQVSARDGDTTHS
                     LAIDDRVPEGYPVLTYQKQERIRLADILGGDVEIFDRLGGTKAMAPIEGLLNRAGLLF
                     VPPVLDSGKTLAESIRDVLASNPEVTYMMTSSNYILDGDGTAVSTNSAYSWSSRDGNR
                     SWNLSVNEAAPDGPELSFASTERIGHAAAVAAANGIADPVKRAEALAALADPLVQISM
                     SVTGDGDPVYQWTSSRDPNESFSISVNTKVPENVNNTPTGTEWTFVRSMRVSRASVLG
                     QMQYLQDPALKQKTLAALDACRNCVFFNEQRSGNDDPVYQWSELGAPNISYSLSMNTG
                     VPSGIPAANGIYPSVKTEATYSVNTRVSRAEVLSRINGDAAGEWIANPEDRAAALAAY
                     NAQVAMNGAFYLARSGTGEEVYQWTVSSDPNTSYSLSFNTAFHSTLYSSSRRVSKEHV
                     AEQYKNLADTDSRKARLAAALKTATVFYVSEGDKGPTYQWTDGSDKNTSYVLSLDRIS
                     PDATAAAPLLTFTVTARESLEQAKARAALIADPVKRAEAMAALEKAGVTVSRSLSDGP
                     AKIDGVDAVYQISDPADANTNYTITMKSGIPVGDPARNTGVEGVWGINTRVSAAEVKS
                     WLENDTLSLIATGVTVAEALAHGESFYLSRGSLAGNEEGDFTDPGQYQWTSFMDPNAS
                     YSLQVREDLKQQTLTKTTQVSAGTVAAELAKAVAAGTITDGDSRKATIEAALADGFSF
                     SMTEGETGPSFQWSSDSDGNKNYNLAVNRKFPDGTTTSGFRTEVSITTSVRASIEEVK
                     NKLALIADESDRAEALAQLEKAGMTVTRSVTDGVGAADGVDPVYQFADLRDPGTNYSI
                     TLKSGIPLGLPDELGNYSKFGSEALWNISIRVGRSEVMSWTEKAGYDLINDAGVDVSS
                     ALSSGALYYFSRSGLDDEGQYQWTSAQDTNVSYSLQPKRDYSQVVLNAATQISREVVR
                     AELDAAVAGGTMTPSDSRYQKILDALAAGVIFTKTTGEKGPAYQWADASDPNTSYGLS
                     VDEHFPTDAGPVRQLSITVSERVSAAVVYAFTDDAASLRITDPEDRREARDLGILAAA
                     FAAEAKGISKLAVTRSLTDGVSAQDGIDPTYQFSDPEAGDVNYSISVKTGLPVDDPAV
                     DRAEEVTWAVNVRVTRKRVEALIDDYIARAEDKNLTAKVEELRTALDLLRKGREIYWS
                     RNGSDDEMGRYQWTNSGDPNVSYTLQAKPGFEDATVAVTTQVSRSAAEAQIALLAAGF
                     KARLDEVEAEMATASAERKVKLQAVLDALTAQRDAEAALIEQALDDADGGIFFRNQQV
                     GSADFSFGWTSLQNRNLSYSLSANTSVPFGRRNEGGGYSNPLTEVTFSRSERVSYSSM
                     MAKARLVTDARKKQEALAAIDSVNDQGGAFYRATTGLKDPVYQWSNPDDSDENWSISV
                     NDRVPQGIADANGVYPSYGTEYSFSHNVRVMESEVLAAIDAIRDAVKKAEARAAFQAA
                     LDENTSFFKSRAGDDDPVYQWSNKSDPNTNYTITFNDHVPSGVRTESGAYPAYTTEAV
                     YSVNRRVSLQDTLALISGIPDAVKRDQAYLALFTADAIYRVQAGNDDPVYQWTNRFDP
                     NESFSMTPNDRLGFTTFSRTARVSREEVAARYARLKDTGDSRYAGLEAALADARDNAF
                     HVSRAGGDDPTYQWTSKTDKNLTYSLSLVKVPVGEALADGSYASYRVEVTFVRNTRAS
                     EADVRAAIALIKDQKGDGAEAAAVLGDLLTLGASVYRSLSDGESPLDGTDPVFQFTGL
                     PDPDQKYSPDKSYSITVNSAAPDGPEATWNISTRVRRTEVARYITDGWFAGDDKDPKL
                     LDALAALADSDDDAFYMSQAGTNDNVQFQWTSKSDPTVSNSIAVKAAWNETTFTKTRR
                     VSKEDVSRAYQYLKDTGDSRYTNLEAALADGELFYMSRAGNDDPGFSWSSRTDRNVSY
                     AMSLNSKVPVGVPETFKSSPEVTFTKSERVKVSEARARARDLLYNTLDREEALAALGP
                     DVTVTKVMTDGEEGQDYIDPVYQFVNTANPDISYSLSIRSGLPVIDSLVDKAVEASWS
                     VAERVGRSDVLSYIEAGADRKEWITDAAKRSAALAALGDGETFYRTRTRSNGEEGATL
                     SYQYQWTSRTNQNVNYSLTVKTGADLADANVLWHESIFSMTRRVSAETVAANYAYLRN
                     EDGTCTSDSRCSNLDAALADGAVFYMSKSGADDASYQWTSRTDRNINYSLSLNSHVGV
                     GLRDSVTGKYPDYKTEVTFSKSERVALSEARAQLSGIGDVSDREEARVALDAGGVVVT
                     RTMTDGEDAQDFVDPVYQFTDVTNPDKSYSITIKSGIPVGLRNGENAYTQFTTEATWG
                     VSQRISKDQVRRHIEKDTAANKEWIADPKKRDEALNALEAGDIFYMSFGGTNDNVTYQ
                     WTDRSDPNVSYSLSVKDRVNPVTGINWHEAVFAKNRRVSEDEVRLELGKLAPSDSRHA
                     ALTAAIADGKIFYMSGAGDDPASYSWTSSSDRNRNYSLSQSVFNDPAGPGGKRIELTL
                     TRSERVSLEEALQMAALIRDESDAEEAGIALRTGGVTVTRAVSDGEGVHDGDDDNPTY
                     QFTDVNNPDRNYSMSINSDAPDGREAVWNISERISREQALQAIDWIVDATRKQQALAA
                     AGGADMFQMSRGGTDDTAIYQWTNRLEDGVNYSLQVEAEWEQAVFNRSERVLVGEFAS
                     HLEALEASYSARRSEIEADILADPRNAQLQLALTALETEYKTQTALIAAALDDGSIFY
                     RSQAGTDDPSFQWTSDSDPNKNYTLGVNTHVPVGEELEDGSYASFRVELNFSENQRIS
                     RNDLAAALTDASGKNLIADLRQKEQADEALADPGAAFFRNTANGKPPTYQWSSS"
BASE COUNT         2486 a         3210 c         3930 g         2334 t
ORIGIN      
        1 gatcgggcgg tgacggtttc gacgtacgag gtgacggggg acgggaaggt gcttcgtgac
       61 gtgatgacct ggaagctcgg cgcggacggt cgattcggga cggcggatga cgttctgacg
      121 ttcgtggagt cgtatgatcc cgtgacgaag gtccgttttc agcagaacta tgacgatcag
      181 ggtcgcgtga tcctttaccg gaacctcggg acaggcgatg aactccgtta tacgttcgac
      241 gacgcggcgg ggaaggtgac gctcacgtcg agcggcaatt atttcaagca ggtctataag
      301 tttgatgagg ctcaccccgg ggatctttcg ttcgcgcgtc tcgtggaaga ggagcgtgac
      361 ggcgtgaaac ggatctggga cgaggacgag cagcttgttt cggagacgga cctcgcgacc
      421 ggcatcgaga cgaaggttgc gagcggtcat tttgtgacga gcgtgacgga taaggacggc
      481 gtgacgacct tttttcggga cggcgtgatc ctttccgtga tcaagcggga cgggacagtg
      541 ctcaggactt accgtcatat cgtggaccgc gggacggggt atctgacgga cgtggatttt
      601 gacgggaagt catcgcttgc gaaagtgtcg atctgtctcg atggggctgg cgccggctgt
      661 tcgaattatc gcgtgatcta cgagaacggc gtgatgctgg agtacgagat gaaaggggac
      721 cgtcctgtgc tcgtttgggt cggtgatccg ctggggacgg aatttacgca gtcgacggac
      781 gcggcgacgg gggaacttac gatccgctat aacgacggtc gcaagagcgt gtactcgcag
      841 gtggcgggtt tttacgagat ccgtcgttcg tactcgttgc gcggggagct tgagacttac
      901 tgttatttcg ggagcgatat ggacggggcg tgcgcctcgg tggatatcga cgcggcgaag
      961 gcgcagatgc gtgccgcggt cggggcgttg ccgggcaccg ggatcgacac gggtacggcg
     1021 aagttcgagg tcccggagct tctcgcgggg gatccgggtc ttgatttttc cgtgaaagcg
     1081 gacgggacgc ttgtgtttta cgcgaagcgg agcgcggagg aatggcagga tattcccggc
     1141 tcgcttttca cgatcggcga gttcggtcgc gtgacggtgt cgcagtatag cggcacggac
     1201 cttcagcaga cgatcgaact gcgcggggat ctgacgctcg cgaacgtcac gaaatatgat
     1261 gaggacagcc gcgtggcggc gtcctacacg tacaacgcgg gcggtacgaa gctcctgacg
     1321 cggagcgtgt acacgtacgt ttccacggac aaggaggaca aggactatcg tctgctgaag
     1381 acggttgaga cctataacgt cggtgatctg aatctccggg atatcgcgaa gcttccggag
     1441 gacgccggga cccttcagtc gcggagtcat tatctcgctc gtcatgcggc gtcgaaagcc
     1501 gcggagggcg aatcgtccgc agtgacggaa tccctgcctg gccgttttga ttatctgggt
     1561 ctcggactgg tggattacac ggaaaactat ctgaaaccgc tcgaggctgg aggggcgccc
     1621 ttgttccagt cgatcgtgcg tcatgagtat gacgtgttcc gtctggtgag gtccgtgacg
     1681 tacaagaacc tgacggggac ctcggtgtgc ggggatgggt tctcggggtg cgaaatgacg
     1741 tcgatggacg agttcacgta ttacgcgggg acggacgaca tcgatttcac gacccgctgg
     1801 tcgatcacga ataacagcct cgatcataaa gtgaagcaga gcgtgttccg caccgaggat
     1861 gtggccgacg aggtgacggt gacgcatgat ttcggcgccg atttcgtcga ggatagtgag
     1921 gacgacgaat tcacggccac gatttcccgc gaggtcggcg acgatctgga cgggaatgct
     1981 tcgatggaga actggggttc tgaggtttac acctacacgg gtatttcgga ttattcattc
     2041 acggttaagg gggggtacaa gcgcgcttac gctggcggtc ttttcacggg tctgaagagt
     2101 cactcgatcc agtatgacgt gtacggtgac ggcacgctga cgcgttcgaa gaacttcgaa
     2161 ttcatccagg ggatcgaaac cgggaaggtg acgttcagcg aatcgcggag gatgacgggc
     2221 gcgatgcgtg ggtatgaagg cacgctcggg gacacgcttc tcggggacct ttcgggggat
     2281 cagcttgagc agtttctcgg tcaggatttc acgttcacgc ggtcgtggca gttcgggttc
     2341 gatagaccgg aagaggaccg gatcttccgt tccgagagtt attccgtgac gagccgcaac
     2401 gagaagatga cgcggacggt ctcgcgcgac gtgatcacgg atcttttgac atacacgcat
     2461 caggaggaga tctcggattc cgcggtccgg tccgagctgg tcgggctcgg agctatcgcg
     2521 gtcacgagcg gggaatccct ttccatgtcg tggagctacg cgggggcctt taacagcgat
     2581 ctccggtttc ttctcgacag gaagatttcg ctggatgttt atggggaagg ttctctcatt
     2641 cgctcgaaga attttgtggt cgagaacggc gctgaaacgg gagagatcaa tttttcggag
     2701 acgaagaaca tcccgcataa cgaaatgtcg tatgacggca cgcataccgt gagtgagctg
     2761 cttctcgggg tggatgccgg gaaggtcgaa tggtcggacc ccgtgtcccg gacgcagacg
     2821 tggacccatg gtttttcgac gcaggtttcg gaccggtatt ttgaagggga gtcctacagc
     2881 ctgacgtccg tggacgggaa gatcacgcgt tccgcgaact gggacgcgat caagggggag
     2941 gttcattaca actatgtgga gcaggtcaag gttccgtacg acacctacac ggtcggcggc
     3001 gtccacgtcg gcgaaaccct catcggggac gtgaaatcga gctcgaactg gaacccgaag
     3061 acgatccttt acatgaccag ttcccgggac tcgttttatg tttcgaaccg gttcattgat
     3121 tccgaatcct atcagatcac gtcccgcgac cgcatggaaa cgcggaccct gagcattgac
     3181 cgctatcatc cggacggtcc caaggtgagt tattccggtt ccgtccgcgt gacgcgcgag
     3241 acggtgacgg ccctgatcgc ggacgaaaag aacacgatcc ttcccggtga cgcgaaagcc
     3301 ttcctggatg tgttcagcga tccggagttc gcgaacaatc ctttttatct tgtgaccggt
     3361 tcgacggacg gtttcaatga tccgggccgc gtgattacga gcaagatgtg tcaggtgtcg
     3421 gcgcgtgacg gtgacacgac tcattctctc gcgatcgacg accgggtacc ggaaggttat
     3481 ccggtgctga cgtatcagaa gcaggagcgg attcgcctcg cggacattct cggcggggac
     3541 gtggagattt ttgaccggct tggcggcacg aaggccatgg cgcctatcga aggtctcctg
     3601 aaccgtgccg gcctgctttt tgtccctccc gttctggact ccggcaaaac cctcgcggaa
     3661 tccatccggg acgttctcgc gtcaaacccc gaagtgacgt acatgatgac ctcgtcgaat
     3721 tacattctgg atggggacgg gaccgcggtc tcgacgaatt ccgcgtattc gtggagttcc
     3781 cgcgacggga acaggagctg gaatttgagc gtcaacgaag ccgctccgga tggtccggag
     3841 ctttccttcg cctcgaccga gcgcatcggt cacgcggcgg cggtggcggc ggcgaacggg
     3901 atcgcggatc ccgtgaaacg cgcggaagcg cttgcggcgc tggcggatcc tttggtgcag
     3961 atttcgatga gtgtgacggg ggacggcgat cctgtctatc agtggacgag ttcaagggac
     4021 ccgaacgaga gtttctcgat ctccgtgaac acgaaagtcc cggaaaacgt caataacacc
     4081 ccgaccggta ccgaatggac ttttgtgcga agcatgcgtg tgagtcgtgc ctccgttctc
     4141 ggacagatgc aatatctcca ggacccggcg ctgaagcaga aaacgctcgc cgcgcttgac
     4201 gcgtgcagaa attgcgtctt tttcaatgaa cagcgctccg ggaacgatga tccggtgtat
     4261 caatggtcgg aactcggcgc tccgaatatt tcgtactcgc tttccatgaa caccggggtt
     4321 ccttcgggga ttccggccgc gaacgggatt tatccgtcgg tcaagaccga ggcgacgtac
     4381 agcgtgaaca cgcgcgtttc gagggccgag gtgctctcgc gcatcaacgg ggacgctgcc
     4441 ggcgaatgga tcgcgaaccc tgaggacagg gccgcggcgc tcgccgcgta caacgcccag
     4501 gtggcgatga atggcgcgtt ctatctggcc cggagcggta cgggtgaaga ggtctatcaa
     4561 tggaccgtga gttcggatcc gaacacgagc tacagtcttt cgttcaatac ggcgttccat
     4621 agcacccttt acagctcaag caggcgcgtc agcaaagaac atgtcgcgga gcaatacaag
     4681 aacctcgcgg atacggattc ccggaaggcc aggctcgccg cggctctgaa gaccgccacg
     4741 gtcttttacg tgtcggaggg ggacaagggc cccacctatc agtggacgga tggatcggac
     4801 aagaacacga gctacgtcct ttctctcgat cgcatatctc ccgatgcgac ggccgcggct
     4861 ccgctcctga catttaccgt gaccgcccgc gagagtctgg agcaggcgaa ggcgcgcgcg
     4921 gcgctgatcg cggacccggt caaacgcgct gaagcgatgg ctgcgcttga aaaggcgggt
     4981 gtgacggtct cccggtcatt gtcggacggt ccggcgaaga tcgatggcgt ggacgcggtt
     5041 tatcagatca gtgatccggc ggatgcgaac acgaattaca cgatcaccat gaaaagcggc
     5101 attcccgtcg gcgatcccgc ccggaatacc ggcgttgagg gggtgtgggg tatcaatacg
     5161 cgcgtgagcg ccgcggaggt caagtcctgg ctggaaaatg ataccctgtc tttaatcgcg
     5221 acgggcgtga cggtcgccga agctctcgcg catggtgaga gtttttatct ttcccgcggg
     5281 agtcttgccg ggaatgagga gggtgatttt acggatcccg ggcagtatca atggacgagt
     5341 ttcatggatc cgaacgcaag ttacagcctt caggtccggg aagacctgaa gcagcagacg
     5401 ctcacgaaga cgacgcaggt gagtgccggg accgttgccg ctgaacttgc gaaagcggtc
     5461 gcggcgggga cgatcacgga cggcgattcc cggaaagcca cgatcgaagc ggcgctcgcc
     5521 gatggttttt cgttttccat gacggaaggc gagaccggtc cgagttttca gtggtcgagt
     5581 gattctgacg ggaacaagaa ctacaatctc gcggtcaatc ggaagtttcc cgacgggacg
     5641 acgacttcgg gtttccgcac ggaagtctcc atcacgacga gcgtgcgtgc cagtatcgag
     5701 gaagtgaaaa acaagctcgc tctgatcgcg gacgagtcag accgcgcgga agcgctcgct
     5761 cagctcgaaa aggcggggat gaccgtgacc cggtccgtca cggacggggt gggcgccgcc
     5821 gacggcgtgg atccggttta tcaattcgcc gatctcaggg acccgggcac gaactacagc
     5881 atcactctca agagcggtat tcccctcggc ctgccggacg aattggggaa ttactcgaag
     5941 tttggtagcg aggctctctg gaacatcagc atccgggtcg ggagatccga agtcatgtcc
     6001 tggacagaga aggccggcta tgatctgatc aatgacgcgg gggttgatgt ttcatcggcg
     6061 ctttcgtccg gagcgctcta ttacttctcc cgttccgggc tggatgatga aggccagtat
     6121 cagtggacga gcgcgcaaga cacaaatgtc agttacagcc ttcagcccaa aagagattac
     6181 agccaagtgg tattgaatgc cgcgacgcag atcagccggg aggtggtccg cgccgaattg
     6241 gatgcggcgg tcgcgggcgg cacaatgacg ccctcggatt cccgctacca gaagatcctt
     6301 gacgctctcg ccgcgggcgt catattcacg aaaacgacgg gtgagaaggg gccggcttat
     6361 caatgggccg atgcttcgga tccgaacacg agctacggtc tttccgtgga tgagcatttc
     6421 ccgaccgatg ccggcccggt caggcagctt tcgatcacgg tgagcgaacg cgtaagcgcc
     6481 gcggttgtct acgcgttcac ggatgacgcg gcctccctca ggatcaccga tcccgaggat
     6541 cgccgcgaag cgcgggacct cggaattctc gcggccgctt tcgcggctga agcgaaaggc
     6601 atttcgaaac tcgcggtgac gcgctccctg accgacgggg tatccgcgca ggacgggatc
     6661 gatccgacgt atcaattttc ggatccggag gccggggatg tcaattactc gatctccgtc
     6721 aagacgggtc tgccggtgga tgatccggcg gttgaccgtg ccgaggaagt gacctgggcg
     6781 gtcaatgttc gtgtgacccg gaagcgggtg gaggccctga tcgacgacta cattgcccgg
     6841 gctgaagaca aaaatctgac cgcgaaggtt gaagaactca ggacggcatt ggaccttctc
     6901 cggaaaggcc gggagattta ttggtcgagg aacggatccg acgatgaaat ggggcgttat
     6961 caatggacga acagcggtga cccgaacgtc agctacacgc ttcaggcgaa acccgggttt
     7021 gaggacgcga ccgttgcggt gacgacccag gtgagccgtt ccgcggcgga ggcccagatc
     7081 gcgcttttgg cggcgggctt caaggcccgc ctggacgagg ttgaagccga gatggcaacg
     7141 gcgagcgcgg agcggaaggt caagcttcag gccgttctcg atgctctgac cgcccagcgc
     7201 gacgcggaag ccgccttgat tgaacaagcc cttgatgatg cggacggcgg gatatttttc
     7261 cgtaaccagc aggtcggttc ggcggacttc tcgttcggct ggacaagcct tcagaaccgg
     7321 aacctgagct atagtctgtc cgccaatacc agcgttcctt tcggccgcag gaacgaaggc
     7381 ggcggttatt ccaatcctct tacggaagtg acgttctcgc gaagcgagcg tgtcagttat
     7441 tcgtcgatga tggcgaaggc ccgtcttgtt actgacgcac gcaaaaagca ggaagcgctt
     7501 gccgcgatcg attcggttaa tgaccagggc ggcgcgttct accgggccac gaccggtctt
     7561 aaggacccgg tctatcaatg gtccaacccg gatgactccg atgagaactg gtcgatcagt
     7621 gtgaacgatc gggtgccgca gggcatcgcg gacgcgaacg gtgtttatcc ctcgtacgga
     7681 acggaataca gctttagtca taacgtgcgg gtgatggaat cggaggtcct ggcggccatc
     7741 gatgcgatcc gggatgccgt caagaaggcc gaggcccggg cggcgttcca ggcggccctt
     7801 gacgagaaca cctcgttttt caaatcccgg gccggggacg acgatcctgt gtatcagtgg
     7861 tcgaacaagt ccgatccgaa cacgaattac acgatcacgt tcaacgatca tgtcccgtcc
     7921 ggtgtccgga ccgagtccgg ggcttatccc gcatatacga ccgaggccgt ttattcggtc
     7981 aaccgccggg taagcctgca ggacacgctc gcattgattt ccgggatccc ggatgcggtg
     8041 aaaagggatc aagcgtatct tgcgcttttt accgcggacg cgatctaccg ggttcaggcg
     8101 gggaatgatg atcccgtcta tcaatggacc aatcgttttg atccgaacga aagtttttcc
     8161 atgaccccga atgatcgtct tggattcacg acgttctccc ggacggctcg ggtgagccgt
     8221 gaggaagtgg ccgcccgata tgcccggctc aaggacaccg gggacagccg gtatgccggg
     8281 cttgaggccg cgctcgcgga tgccagggat aatgcgttcc acgtttcccg ggccggcggt
     8341 gacgatccca cgtatcagtg gacgagcaag acggacaaga acctgaccta cagtctttcg
     8401 ctcgtcaagg tcccggtcgg ggaagcgctc gcggacggga gttacgcgag ctaccgtgtg
     8461 gaggtgacgt ttgtccggaa cacgcgcgcg agcgaggcgg acgtgcgtgc cgcgatcgcc
     8521 ttgatcaagg atcagaaagg cgacggcgcc gaagcggccg cggtcctcgg ggatcttctc
     8581 acgttgggcg cgtcggttta tcgatcgctt tcggacggag agtccccgct tgacgggacg
     8641 gatcctgtgt tccagttcac gggactcccg gatcccgacc agaaatattc cccggacaaa
     8701 agttattcga tcacggtgaa ttccgcggcg ccggacggtc cggaggcgac ctggaatatt
     8761 tcgacgcggg tccgcaggac tgaggttgcc cgatatatca cggacggctg gttcgcgggc
     8821 gacgacaagg atccgaagct gctggacgcg cttgccgcgc tcgcggactc ggacgacgac
     8881 gctttttaca tgtcgcaggc gggtacgaac gacaacgttc agttccagtg gacgagcaag
     8941 agcgatccga cggtcagtaa cagcatcgcg gtcaaggcgg cctggaatga aacgacgttc
     9001 acgaagacgc gccgggtgag caaggaggat gtctccaggg cgtatcagta tctcaaggat
     9061 acgggcgaca gccggtacac gaacctcgaa gccgcgctcg cggacggtga gcttttctat
     9121 atgtcccggg cgggtaatga tgaccccggt ttttcctggt ccagccggac ggacaggaat
     9181 gtaagctacg cgatgtccct gaacagcaaa gtccctgtcg gggtgcccga aaccttcaag
     9241 tcttcgccgg aagtcacgtt cacgaagagc gagcgcgtca aggtgagtga agcgagagcc
     9301 cgggcccggg accttcttta taacacgctc gacagggagg aagcgcttgc cgccctcggc
     9361 ccggatgtga cggtcacaaa agtcatgacg gacggcgagg aaggccagga ttacattgat
     9421 ccggtgtacc agtttgtgaa cacagcgaac cccgacataa gctacagcct ctcgatccgg
     9481 agcgggctcc ccgtcatcga ctccctggtg gacaaggcgg ttgaagcttc gtggagcgtt
     9541 gcggagcgcg tgggccgctc ggacgttttg agctatatcg aagcgggagc ggaccggaag
     9601 gagtggatca cggacgccgc gaaacggtct gccgcgctcg ccgcgctcgg ggacggcgag
     9661 actttttacc ggacgcgaac ccgctcgaac ggtgaggaag gtgcgacgct ttcctatcag
     9721 tatcagtgga cgagccgcac gaaccagaat gtgaactaca gcctcaccgt gaagacaggg
     9781 gccgaccttg ccgacgcgaa tgtcctttgg cacgagtcga tcttctccat gacgcggcgg
     9841 gtcagcgctg agaccgtcgc cgcgaattac gcgtatttaa ggaatgaaga cggcacctgc
     9901 acgagtgaca gccgatgctc gaaccttgac gccgcgcttg cggacggcgc ggtcttttat
     9961 atgtcgaagt cgggtgccga tgacgcgagc tatcagtgga cgagccgcac ggaccggaac
    10021 atcaattaca gtctttcgct gaattcgcac gtcggggtcg ggttgaggga ttctgtgacc
    10081 gggaaatatc cggattataa gaccgaggtg actttttcaa agagcgagcg tgtggcgctt
    10141 tctgaggccc gcgcgcagct ttccgggatc ggggatgtgt cggatcgtga ggaagcccgc
    10201 gtggcgcttg atgccggcgg cgtcgtggtc accaggacca tgaccgacgg cgaggacgcg
    10261 caggatttcg tggatcccgt ctaccagttc acggatgtga ccaatccgga caagagctac
    10321 agcattacga tcaaaagcgg aattcccgtc ggtctccgta atggcgaaaa cgcgtataca
    10381 cagtttacga cggaggcgac ctggggtgtg tcgcagagga tcagcaagga ccaggttcgc
    10441 cgtcatatcg agaaggatac ggccgcgaac aaggagtgga tcgcggatcc gaagaagcgg
    10501 gatgaggcgc ttaacgcgct tgaagccggg gacatttttt atatgtcttt tggcggaacg
    10561 aatgacaatg tcacctatca gtggacggac cgctccgatc cgaacgtgag ctacagcctg
    10621 tccgtaaaag acagggtcaa ccccgtgacg gggatcaact ggcatgaggc ggttttcgcg
    10681 aagaaccgcc gcgtgagcga agatgaggtc cgtttggagc ttggcaagct cgcgccgtcc
    10741 gacagccggc atgcggcctt gaccgcggcg atcgccgacg ggaagatctt ctatatgtcc
    10801 ggggccggcg atgatccggc ctcttactcc tggacaagct cctcggaccg caacaggaat
    10861 tacagcctga gccaatccgt gttcaatgat cctgccggac cgggcgggaa gcggattgaa
    10921 ctgaccctga cccgcagtga acgggtgagt cttgaggagg cgctccagat ggccgccctt
    10981 atccgggatg agtccgatgc cgaagaagcg ggtatcgcgc tgcgaacggg cggggtcacg
    11041 gtcacccgcg cggtttcgga cggagagggc gttcacgacg gcgatgatga caatcccacg
    11101 tatcagttca cggatgtcaa caatccggac cggaattaca gcatgagcat caactcggac
    11161 gctcctgacg gccgggaagc ggtctggaat atttcggagc ggatcagccg ggaacaggcc
    11221 ctgcaggcga tcgactggat cgtggacgcg accaggaaac agcaggcgct cgcggcggcc
    11281 gggggtgcgg atatgttcca gatgtcgcgc ggcgggaccg acgataccgc gatctatcag
    11341 tggaccaacc gcctggagga cggcgtcaac tacagcctgc aggttgaggc ggaatgggag
    11401 caggcggtct ttaacagaag cgaacgggtg cttgtcggcg agtttgcctc ccacctggaa
    11461 gcgttggaag cctcttattc agcgcgtcgc tcggaaattg aggcggatat tctggccgat
    11521 ccccggaacg cccagcttca gttggcgctg accgcgcttg aaacggaata taaaacgcag
    11581 acggcgctga tcgcggcggc gctcgatgac ggaagtatct tttacagatc tcaagccggg
    11641 accgatgatc cgagtttcca gtggacaagc gattcggacc cgaacaagaa ctacacgctc
    11701 ggtgtgaaca ctcacgtgcc ggtcggcgag gagctcgagg atggaagcta cgcgagcttc
    11761 agggtggagc ttaatttttc ggagaatcag cgtatttccc ggaacgatct tgccgccgcg
    11821 ctcacggacg cttccggaaa aaatctcatc gcggacctgc ggcagaagga gcaggcggat
    11881 gaggcgctcg cggatcccgg cgccgctttc ttcaggaata ccgcgaacgg gaaacccccg
    11941 acgtatcagt ggtccagctc
//
LOCUS       DAOOBX010000002        10204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_8223_length_10204_cov_4.248005, whole genome shotgun sequence.
ACCESSION   DAOOBX010000002 DAOOBX010000000
VERSION     DAOOBX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8223_length_10204_cov_4.248005"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /gene="polA"
                     /locus_tag="PLY30_00010"
     CDS             <1..1701
                     /gene="polA"
                     /locus_tag="PLY30_00010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPW64512.1"
                     /translation="STDPMRAHLVGMSFAIEPHRAFYVPTASKEHPGPGLPLAEVLRG
                     LKLVLEDAKIGKCGQNIKYDAMVMARHGIELRGVRFDTMIASYLIDPIKRNHNLDDIS
                     LEHLNVKKIPVETLIGKGKTRISMAEVPLEKITEYACEDADCVLRLAPVLKRKLAELD
                     LAGLYEKVELPLAGILGKMETNGVALDTVLLGQLSRKTGEEIDGLLQKIHAEAGEEFN
                     VDSPKQLAEILFVKKKIPSMKKTKTGYSTDASVLEKLALSHELPRLVLEYRERAKLRS
                     TYLDALPEMVNPETGCVHTSYHQTTTDTGRLSSSEPNLQNIPVKTEAGRLIRKAFVSR
                     RKTGKILSADYSQIELRILAHFCEEPNLVKAFEEDRDIHAFTATLLYDVREKDVTREM
                     RNVAKTINFSVLYGKTSFGLSQDLQISIQEADAFIRNYFGRYAKVRDFLESQKEQARK
                     AGYLTTILGRRSYFPNLRSSNVALRNYAERAAINAPLQGSAADLIKLAMIRVQGLLES
                     EKSEALMIMQVHDELVFDVPAAGVEALGRMVKKEMEGAYALKVPLKADVAAGDSWYKN
                     "
     gene            1686..2312
                     /gene="coaE"
                     /locus_tag="PLY30_00015"
     CDS             1686..2312
                     /gene="coaE"
                     /locus_tag="PLY30_00015"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPW64513.1"
                     /translation="MVQKLIVGLTGGFGSGKSTVAKAFRGLGAATIDADKVAHAALRK
                     SSPLFKKIAALFPEALSGCGRYFHREKIAEKVFADPAKRERLEALVHPYVYREIRKMA
                     AAAKRKVVIVEVPLLFEAGFEGLCDWTVVVTCKPSTRMARLKSKGFSPVQIKAREKAQ
                     MKESLKAAKADFVIDNSGSESKTGKSAKRLWERLALLRRQKPARFYDR"
     gene            2534..3820
                     /gene="rho"
                     /locus_tag="PLY30_00020"
     CDS             2534..3820
                     /gene="rho"
                     /locus_tag="PLY30_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPW64514.1"
                     /translation="MRQQKAGEKPLDVVGLRKAKIAKLQEMAHKLNVENASGLKKQDL
                     IFKILQAQAQQSGLMFGEGVLEILEDGFGFLRSPNYNYLPSPDDIYVSPSQIRRFNLR
                     TGDVVSGLIRPPKEGERYFALLKVELINFEETDNAKDKIAFDNLTPLYPNKRIILETV
                     TEEVSTRVMDLLTPVGFGQRGMIVAQPYSGKTILLQKIANSITTNHPDAYLIVLLIDE
                     RPEEVTDMQRSVKGEVIASTFDEPADRHVQVAEIVLEKAKRLVEHKRDVIILLDSITR
                     LARAYNTVAPHSGKILSGGVDSNALHKPKRFFGSARNVEEGGSLTVIATALVDTGSRM
                     DEVIFEEFKGTGNMELQLDRNLFQRRIYPAIDVKKSNTRKEDLLMPADELNRVWILRK
                     VLNELNPTEAMELLVDRLRKTKSNAEFLLKMNQAEK"
     gene            3823..4143
                     /gene="rpmE"
                     /locus_tag="PLY30_00025"
     CDS             3823..4143
                     /gene="rpmE"
                     /locus_tag="PLY30_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPW64515.1"
                     /translation="MKKDIHPNYQPSTIDCACGNSIRTRSVMPRIQVDVCSACHPFFT
                     GKQKLMDTAGRIDRFKKKFQDKIVGAKKSTKAAPVSALRAKAADMRKANLQALRDKVK
                     AEGK"
     gene            4223..5299
                     /gene="prfA"
                     /locus_tag="PLY30_00030"
     CDS             4223..5299
                     /gene="prfA"
                     /locus_tag="PLY30_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPW64516.1"
                     /translation="MRIRERLEKAEARFLAVEALLAQPETARDTAQLQKLSKELAQLR
                     PIVEGFRKYRKLEAEQAELRTNLERGQEDPAMRGLIEEEVVSLDGQIEKTNLELENLI
                     LEGCEPDAARSVIIEIRAGTGGEEAALFVADLYRMYSQFAAGHGLKTEVMDLSPTGIG
                     GLKEVVFGVTGEHAYSLFKYESGIHRVQRVPKTEASGRVHTSAVTVAVLPEADETEID
                     IQPNDLKIDVYRASGAGGQHVNKTESAVRITHIPTGFVVACQDERSQHKNKAKAMRVL
                     RAKIYEFQKEKQEAEIAKNRKDQVGSGDRSGKIRTYNYPDQRLTDHRIGLTVHNLPVI
                     MEGKLEEVVKALAEDEKVRKLTNG"
     gene            5301..6179
                     /gene="prmC"
                     /locus_tag="PLY30_00035"
     CDS             5301..6179
                     /gene="prmC"
                     /locus_tag="PLY30_00035"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPW64517.1"
                     /translation="MAMTRAALLAAGRDALARAGIGEAKEEAERILENVLKASKAALF
                     LDAAKEVAPEAERHFLRIVEERKTRKPLAYLLGEACFGEEILEVSDRCLIPRPETEIL
                     VEALAKQLAPEREFSFLDLGTGSGAIAIALMRKFPLSKGTLSDISEEALGIARRNLGR
                     YGLENRALLKRGDLFGAFAGEEPWDVVVSNPPYLSEGDFRNLQPELAFEPRLALDGGT
                     DGFDFYRRIIPEARRYLKPGGFLALEAGAGQADQVSKWLQGAGYDNILRLKDYLGFDR
                     VVMARINEGLNGKTYH"
     gene            6160..7425
                     /gene="murA"
                     /locus_tag="PLY30_00040"
     CDS             6160..7425
                     /gene="murA"
                     /locus_tag="PLY30_00040"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPW64518.1"
                     /translation="MEKLIIKGGARLIGEVEVSGSKNAVLPIMAAALLSEGESIIENV
                     PDLWDVQTMVRLIRSLGAKAILENGVLKIRPGNNLKPFAPYKLVKTMRASVCVLGPLL
                     ARLGEAEVSLPGGCAIGPRPIDLHLKGLEALGAEIEIDHGYVRTKAKKSLRGTEIYLG
                     GGYGSSVLATDNVLMAAVLAAGRTVIENAACEPEVVDLANFLRKMGAKIQGDGSPRIE
                     VEGVKKLRGVSYRVIPDRIEAGTYMFGAAITNGDVLIRKMEPAHLNAVIDKLHQAGMK
                     VEKYKNAVRVKRTGKLRPVDVTTLPYPAFPTDLQAQAMALMTVTPGISIITEKIYPNR
                     FMHVPELGRMGAKIFLEGASAIVHGVKKLSGAPVMASDLRASAALVLAALVAQGETEV
                     QRIYHLDRGYENLEMKLNSLGAQVRREKE"
     gene            7506..7763
                     /gene="rpsP"
                     /locus_tag="PLY30_00045"
     CDS             7506..7763
                     /gene="rpsP"
                     /locus_tag="PLY30_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPW64519.1"
                     /translation="MSVKIRMKRFGTKDKAKWRVVVCDSKSPRDGRFIEEIGYYDPLP
                     KQEKLSLKEARLEYWVKNGAQVSEALKSLIRRQKKKAKAAK"
     gene            7783..8478
                     /gene="trmD"
                     /locus_tag="PLY30_00050"
     CDS             7783..8478
                     /gene="trmD"
                     /locus_tag="PLY30_00050"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPW64520.1"
                     /translation="MLSIDVVTLFPEFFKSPLGESLLKKAQDRGLVKVRVHDLRDWTH
                     DRHKTADDKPFGGGAGMVMKPEPIFECVEAVAPKGFVVLLDPQGEPMSQKLARKLAKK
                     KHLVLIAGHYEGVDQRVKDHLVDQQISVGDFITMGGEAPALCVIEAVARLVPGVIGNE
                     ESLARESFECGMLEHPQYTRPREYRGWKVPNVLVSGYHKEVEAWRKKEAEKVTKERRP
                     DLLTSSRRKSWAK"
     gene            8463..8777
                     /gene="rplS"
                     /locus_tag="PLY30_00055"
     CDS             8463..8777
                     /gene="rplS"
                     /locus_tag="PLY30_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPW64521.1"
                     /translation="MMGKIKLIEKELESKKIPEFRVGDTLKVHIKVKEGDKSRLQLFE
                     GICIRKKGRGANASFSVVKESYGDMVEKLFPLYSPSIEKITVASKGKARRAKLYHFKK
                     KK"
     gene            8842..9582
                     /locus_tag="PLY30_00060"
     CDS             8842..9582
                     /locus_tag="PLY30_00060"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPW64522.1"
                     /translation="MKRVKDLPVNEYERDGSANEAPSFSKGESFSRETARRECLGRLL
                     EFDRDILASGWMAGVDEAGRGPLAGPVVAAAVILERADELAGINDSKKLSPVSREKLF
                     LKIVRHALVGVGTADERTIDTINIYEATRLAMKRAVLALPHTPAFVLIDGPLRLDLPL
                     PQRGIVSGDQKSASVAAASIVAKVFRDRWMKHLHGLYPAYAFHAHKGYGTAEHLEALQ
                     AFGPCPVHRRSFAPVAFSEKGPFEKALT"
     gene            9579..9974
                     /locus_tag="PLY30_00065"
     CDS             9579..9974
                     /locus_tag="PLY30_00065"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPW64523.1"
                     /translation="MKIAPNPKPFRYSVGDRGEVCAAEYLRKKGFKVLQANYRCALGE
                     IDLVAEKAGRIHFVEVKTRSGKGFGSPEAAVDLRKQVKLVKLAAWYLKDKRLQDPPVS
                     FDVMGITLEEGLAPEIRFFEGAFEKGEVW"
     gene            9971..>10204
                     /locus_tag="PLY30_00070"
     CDS             9971..>10204
                     /locus_tag="PLY30_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64524.1"
                     /translation="MSGFFSRVFSRLVRPFAEVHPGERTKTFLMFCYFLLTISTIYIL
                     KPVRSSLFLSEFSADKLRYVNIGEGLFLIAVVWL"
BASE COUNT         2392 a         2700 c         3202 g         1910 t
ORIGIN      
        1 tcgacggacc ccatgcgcgc gcaccttgtc gggatgagct tcgcgatcga gccgcacagg
       61 gccttctatg tcccgacagc aagcaaagag catccgggcc ccggtcttcc cttggcggaa
      121 gtgctccggg ggctgaagct tgtgctcgag gacgcgaaga tcggaaagtg cgggcagaac
      181 atcaaatacg acgccatggt catggcgcgc cacggcatcg agctccgcgg cgtccggttc
      241 gacacgatga tcgcgagcta tctgatcgat ccgatcaagc gcaatcataa cctggatgac
      301 atttcactcg aacacctgaa cgtcaaaaag attccggtcg agacgttgat cggaaagggg
      361 aagacccgga tcagcatggc cgaagtcccg ctcgaaaaga tcacggaata cgcctgcgag
      421 gacgccgact gcgttctgcg gctcgcgccg gtcctcaaaa ggaaactcgc ggagctggat
      481 ttggccggac tttatgaaaa agtcgaactg cccctcgcgg gtatcctcgg gaagatggag
      541 acaaacggcg tcgcgcttga caccgtcctt ctcggacagc tttcccggaa aacgggcgag
      601 gagatagacg gtcttctgca gaaaatccac gccgaggcgg gcgaagaatt caatgtcgat
      661 tcgccgaaac agctggcgga gattcttttc gttaaaaaga agattccgtc gatgaaaaag
      721 acgaagaccg gctactcaac ggatgcgagc gtgctcgaaa agctcgcgct ttcacatgag
      781 cttccgcgtc tcgttctgga ataccgggaa cgcgcgaagc tgcggtccac gtatcttgac
      841 gcgcttccgg aaatggtgaa tccggagacg gggtgcgtgc acacgtccta tcaccagacc
      901 acgaccgaca cggggcgtct gtcgagttcg gagccgaatc ttcagaatat cccggtcaag
      961 accgaggccg ggcgcctgat ccggaaagct ttcgtctccc gtcgcaagac ggggaagatt
     1021 ctctccgccg actattcaca gatcgagctc cggatcctcg cgcatttttg cgaggagccg
     1081 aatcttgtga aggcgttcga ggaggatcgc gacatccacg cgttcacggc gacgcttctg
     1141 tacgatgtga gggaaaagga cgtgacgcgc gaaatgcgca acgtcgcgaa gacgatcaac
     1201 ttcagcgtcc tttacgggaa aacgagcttc gggctttccc aggacctcca gatttcaatc
     1261 caggaagccg atgccttcat ccggaattat ttcggacgct acgcgaaggt gagggatttc
     1321 ctcgaatcgc agaaggaaca ggccaggaag gctggttacc ttacgacgat cctcggccgg
     1381 aggtcctatt tcccgaatct ccgttcgtcg aacgtcgcac tcaggaatta tgccgagcgc
     1441 gccgcgatca acgcacccct gcaaggttca gcggcggatc tgatcaagct cgcgatgatc
     1501 agggtccagg gccttctgga gagcgaaaaa agcgaagcat tgatgatcat gcaggtccat
     1561 gacgaacttg tctttgatgt gccagccgcc ggcgttgagg cgctcggacg gatggtcaaa
     1621 aaagagatgg agggcgctta cgcgctgaag gtgccgctca aggcagacgt ggccgcggga
     1681 gattcatggt acaaaaactg atcgttggtc tgaccggcgg gttcgggagc gggaaaagca
     1741 cggtcgcgaa ggctttccgg ggtctcgggg cggcgacgat cgatgccgac aaggttgctc
     1801 acgcggctct caggaaaagc agtccgctct ttaaaaagat cgccgcgctt ttcccggagg
     1861 cgctcagcgg atgcgggaga tattttcacc gggaaaagat cgccgaaaag gtttttgccg
     1921 atccggcaaa gagggagcgg ctggaagccc tggtccaccc gtacgtgtac cgggagatca
     1981 ggaaaatggc cgctgccgcg aaaaggaaag ttgtgatcgt ggaagtgccg cttcttttcg
     2041 aagcgggttt tgaaggcctc tgcgactgga cggtcgtcgt gacctgcaag ccgtcgacca
     2101 ggatggcccg gctgaaaagc aaaggtttct caccggtcca gatcaaggcg cgcgaaaagg
     2161 cccagatgaa agaatccctg aaggccgcga aagcggattt tgtgatcgat aactcaggtt
     2221 ctgaatcaaa aaccggaaaa agcgcgaagc gtttatggga acgtctcgcg cttttaaggc
     2281 gccagaagcc ggcccgtttt tacgaccggt gacctgaagg tctatttaaa aagtaactaa
     2341 cagaagtaca accaaccgtt agatgcattt gggcttctga cggggcaagg ggagtgaccc
     2401 aatgatgcaa aaagaacgga atcaccatcc acacaatcgg agaagtgacg atcaccacgg
     2461 cgctccgagg cagccgaggc cgcagatgcg cgacgatgac gcgcagatca acggcgaggc
     2521 gctcgaaaag gcgatgcggc agcaaaaggc cggcgagaaa ccactcgacg tggtcgggct
     2581 gaggaaggcg aagatcgcga aactccagga gatggcccat aagctgaacg tcgagaatgc
     2641 ctcgggactc aagaagcagg atctgatctt caagattctc caggcgcagg cccagcaatc
     2701 ggggctcatg ttcggagagg gcgtcctgga gattctcgag gacggcttcg ggttcctccg
     2761 ttccccgaac tataattacc tgccgtcgcc ggatgatatc tacgtcagcc cgagccagat
     2821 ccgccgtttc aacctgcgga ccggcgatgt cgtgagcggc ctgatccgtc ctccgaaaga
     2881 aggagaacgg tatttcgccc tgctcaaggt cgagctgatc aatttcgagg agacggacaa
     2941 cgcgaaggac aagatcgcct tcgacaacct gactccgctc tatccgaaca aacggatcat
     3001 actcgagacg gtgacagaag aggtctcgac gcgcgtgatg gacctcctca cacccgtcgg
     3061 tttcggtcag cgcgggatga tcgtcgcgca gccctacagc ggcaagacaa tcctcctcca
     3121 gaagatcgcc aactccatca cgacgaacca tccggacgcg tacctgatcg tgcttttgat
     3181 cgatgaacgt cccgaggaag tgacggacat gcagcgctcc gtgaagggcg aggtcatcgc
     3241 ttcgaccttt gacgagcccg cggaccgcca cgtgcaggtc gccgaaatcg tgctcgagaa
     3301 ggcgaaacgt ctcgtggaac acaaacgcga tgtgattatc ctgctcgact cgatcacccg
     3361 tctggcgcgc gcctataaca cggtcgcgcc gcactccggg aagatcctct cgggcggtgt
     3421 ggactccaac gcgctccata aaccgaaacg ctttttcggt tcggcgcgta atgtcgagga
     3481 aggcggcagc ttgaccgtca tcgcgacggc gctcgtcgac acggggtccc gcatggacga
     3541 ggttattttc gaagagttca agggcaccgg aaacatggag ctccagcttg accggaacct
     3601 cttccagcgc cggatttatc ccgcgatcga cgtcaaaaag tcgaacacgc gtaaggaaga
     3661 cctcctgatg ccagccgacg agctcaaccg cgtctggatc ctcaggaaag tcttgaacga
     3721 gcttaatccg accgaggcca tggagcttct ggtcgaccgt ctgcgcaaga ccaaatcgaa
     3781 cgcggaattc cttcttaaaa tgaaccaagc ggagaaataa aaatgaaaaa agacattcac
     3841 ccgaactatc aacccagcac catcgactgc gcctgcggca actccatcag gacgcggagc
     3901 gtgatgccgc gtatccaggt cgatgtttgc tcggcctgcc atccgttctt taccggtaaa
     3961 cagaagctga tggacaccgc cggtcgcatt gaccgtttca agaagaagtt ccaggacaag
     4021 atcgtcggcg ccaagaaatc gacgaaggcc gcccccgtgt cggcactgcg cgcgaaagcg
     4081 gcggacatga ggaaggcgaa tctccaggcc ctcagggaca aggtcaaggc ggaagggaag
     4141 taagaacaca agccgcaagg ccgcgaagcc acaagaatgg atttttatgt ggcgcgcgtc
     4201 ttcgtgacgg gtggtgaccg ccatgcgaat ccgtgaacga cttgaaaaag ccgaggcccg
     4261 tttcctggcg gtcgaggccc tgctcgcaca gccggaaacg gcccgcgata ccgcgcagct
     4321 gcagaagctg agcaaggagc tcgcgcagct tcgtccgatc gtcgaaggct tccggaaata
     4381 ccggaagctt gaggcggaac aggccgaact gagaacgaac cttgagcgcg gccaggaaga
     4441 cccggccatg cgagggctga tcgaggaaga ggttgtcagt cttgacggcc agatcgagaa
     4501 gaccaacctc gagctggaga atcttatcct agaaggatgc gagccggatg ccgcgcgaag
     4561 cgtgatcatc gaaatccgcg ccggaacggg cggggaagag gccgctcttt tcgtggcgga
     4621 cctctatcgt atgtattcgc agttcgcggc cgggcacggg ctgaagaccg aagtgatgga
     4681 tctgagcccg accggaatcg gaggattgaa ggaagttgtt tttggcgtga cgggcgaaca
     4741 cgcttacagc cttttcaagt acgaaagcgg aatccaccgg gtgcagcgtg ttcccaaaac
     4801 cgaagcgagc ggacgagtcc acacttcggc cgtgacggtc gcggtgttgc cggaagcgga
     4861 cgagaccgag atcgacatcc agccgaacga tctcaagatc gacgtgtatc gcgcgtcggg
     4921 ggccggcgga cagcacgtga acaagaccga atcggccgtg aggatcacgc atatcccgac
     4981 ggggttcgtt gtcgcgtgcc aggacgagcg gtcccagcac aagaacaagg ccaaggcaat
     5041 gcgcgtgctg cgtgcgaaga tttacgagtt ccagaaggaa aaacaggaag ccgagatcgc
     5101 gaaaaaccgc aaggaccagg tcggctcggg cgaccggtca ggcaagatac ggacctataa
     5161 ttatccggac cagcgcctga cggatcaccg gatcgggctc acggttcaca atcttcccgt
     5221 gatcatggaa gggaagctgg aagaggttgt gaaagcgctc gcggaagatg aaaaggtccg
     5281 caaactgacg aacggatgat atggcgatga caagagcggc gctccttgcc gcgggacgcg
     5341 acgcgttggc gcgcgccggg atcggggaag cgaaagagga agcggaacgg attctggaaa
     5401 atgtcctgaa ggcttccaaa gccgctttgt ttctggacgc ggcaaaagaa gtcgctccgg
     5461 aagcagagcg gcattttttg aggattgtcg aagaacgcaa gactcgtaag ccgctcgcgt
     5521 atcttctcgg cgaagcctgt ttcggagagg aaattctcga ggtcagtgac cgttgcctga
     5581 taccgagacc cgagaccgag attctcgttg aagcgcttgc gaaacaactt gcgcccgagc
     5641 gggagttttc gttcctggac ctcgggaccg ggtccggagc gatcgcgatt gcccttatga
     5701 ggaagttccc cctttcaaaa gggacgttgt cggatatttc ggaggaggct ctcgggattg
     5761 cccgaaggaa cctggggcgt tacggtctgg agaacagggc gctactgaag cggggggatc
     5821 ttttcggggc gtttgccggt gaagagccct gggatgtggt agtttcgaac ccgccgtacc
     5881 tgagtgaggg cgatttccgg aatctccagc cggagcttgc gtttgagccc cggctggccc
     5941 ttgacggcgg gaccgatggt tttgatttct accggagaat cattcctgag gccaggagat
     6001 atctgaaacc gggcgggttt ttagccctcg aagcgggggc ggggcaggcg gatcaggttt
     6061 caaagtggct tcagggcgcg ggttatgata atattctgcg cttaaaagac tatttaggct
     6121 ttgacagggt tgtcatggcc cggattaatg aaggactgaa tggaaaaact tatcattaaa
     6181 ggcggcgccc ggctgatcgg cgaggtcgaa gtttcggggt ccaagaacgc ggttctgccg
     6241 atcatggcgg cagcgcttct gtccgaaggg gagtcgataa tcgagaacgt cccggacctg
     6301 tgggacgtcc agaccatggt gaggctcatc cggtctctcg gggccaaagc gatcctcgag
     6361 aacggcgttc tcaagatcag gccggggaac aatctgaaac cgttcgcgcc ttataaactg
     6421 gtcaagacca tgcgcgcgtc agtttgtgtg ctgggtccgt tgctcgcgag gctcggggag
     6481 gccgaggttt cgctcccggg gggatgcgcg atcggcccgc ggccgatcga tcttcacctg
     6541 aaaggtctcg aagcccttgg agccgagata gagattgatc acggttatgt gcggacaaaa
     6601 gcgaagaaat cgcttcgcgg gaccgaaatc tatctcggcg gcgggtacgg atcttcggtg
     6661 ctcgcgacgg acaacgtgct gatggctgcc gtgctcgcgg ccgggcggac ggtgatcgag
     6721 aatgcggcct gcgaacccga agtggtcgat ctggcgaatt ttttgagaaa gatgggcgca
     6781 aagatacagg gggatggttc gccgaggatc gaggttgaag gtgtgaaaaa attgcgcggt
     6841 gtgagctatc gcgtgattcc ggacaggatc gaggcgggga cctatatgtt cggcgcggcg
     6901 atcacgaacg gcgatgttct gatccggaag atggagccgg cgcatctgaa cgccgtgatc
     6961 gacaagctcc atcaggccgg catgaaggtc gagaaataca agaacgcagt acgcgtgaaa
     7021 cggacgggga agctccggcc ggtcgatgtg acgaccctgc cctatccggc gttcccgacg
     7081 gatttgcagg cccaggcaat ggcgcttatg accgtgacgc cggggatcag cattatcacg
     7141 gaaaaaatct atccgaaccg gttcatgcac gtcccggaac tcggtcgtat gggcgccaag
     7201 atttttctgg agggtgcgag cgcgattgtg cacggcgtga aaaaactgag cggggcgccc
     7261 gtgatggcgt ccgaccttcg cgcaagcgcg gcgctcgttc tggcggccct cgtggcgcaa
     7321 ggggagacag aagtccagcg catctatcat ctggatcgcg ggtatgagaa tctggagatg
     7381 aaactgaact cgctcggggc tcaggtccgg agagaaaaag aataatcaaa agaatcgcag
     7441 agcgaaaagc ggatggagta aaaatcaaac gctggacgtt ccgcgttaaa aaccaaggag
     7501 cagaaatgtc agtcaagatc agaatgaaac ggttcggcac gaaggacaag gccaagtggc
     7561 gtgtggtggt gtgcgactcc aaatcgcccc gcgacgggcg ctttatcgag gagatcggtt
     7621 attacgatcc gcttccgaag caggagaaac tgtcgctcaa ggaagcgcgt ctcgagtatt
     7681 gggtcaagaa cggcgcccag gtaagcgagg ccctgaagag ccttatccgc cggcagaaga
     7741 aaaaagccaa agccgcgaaa taaccgcctc cgggatcgca gcgtgctatc catagatgtg
     7801 gtgacgcttt tcccggagtt ctttaaaagc ccgctgggcg agtccttgtt gaaaaaggca
     7861 caggatcgcg ggcttgtgaa ggttcgggtc cacgatctcc gggattggac gcatgaccgg
     7921 cacaagaccg cggacgacaa accgttcggg ggaggggccg ggatggtcat gaagcccgag
     7981 ccgatctttg aatgcgtgga agcggtcgcc ccgaaaggat ttgtagtgct tctggacccg
     8041 cagggcgaac cgatgagcca gaagctggcg cgcaagctcg cgaagaagaa gcatcttgtg
     8101 ctgatcgcgg gccactacga aggcgtcgac cagcgcgtga aggaccacct ggtcgaccag
     8161 caaatctcgg tcggggattt tatcacgatg ggcggcgagg cgccggccct ttgcgtgatc
     8221 gaggcggtcg cgaggctggt cccgggggtt atcgggaacg aagaatcgct cgctcgcgaa
     8281 agttttgaat gcggcatgct ggagcatccg cagtacacac ggccccggga ataccggggg
     8341 tggaaggtgc ccaacgtgct ggtgtcgggg tatcacaaag aagtcgaagc ctggcgaaaa
     8401 aaagaggccg agaaggtcac gaaggaaaga cggcctgatc tgttaacatc ttcaaggaga
     8461 aaatcatggg caaaataaaa ttgatcgaga aagagcttga gtcaaagaag attcccgagt
     8521 tccgcgtcgg ggacacgctg aaggtccata tcaaggtcaa ggaaggcgac aagtcccgcc
     8581 ttcagctttt tgagggtatc tgcatccgca aaaagggccg cggcgccaac gcgagttttt
     8641 cggtcgtcaa ggaaagctac ggagacatgg tcgagaagct tttcccgctc tactcgccgt
     8701 cgatcgagaa gatcacggtc gcttcgaagg gaaaggcccg ccgcgcaaag ctctatcact
     8761 tcaagaagaa aaagtaatcg cttcggggtt cgttatgacg aaccccgcga atgagccgtg
     8821 aacggatgtc gcggttttgg ggtgaaaaga gtgaaagacc tgccggtcaa tgagtatgag
     8881 agagacggga gcgcgaatga ggcgccctct ttctccaagg gagaaagttt ttcaagggaa
     8941 acggcccgtc gcgagtgtct cggaaggctc ctcgagtttg accgggatat ccttgcttcg
     9001 ggatggatgg ccggagtgga tgaggccggc cggggaccgc ttgcggggcc ggttgtcgcg
     9061 gcggctgtga tcctggagag ggccgatgag ctcgcgggaa tcaatgactc caagaaactc
     9121 tcacccgtct cccgcgaaaa actgtttctc aagatcgtgc gtcatgccct tgtcggtgtg
     9181 ggcacggcgg acgaaagaac gatcgataca atcaatatct atgaagccac gcgccttgcc
     9241 atgaagcggg ccgtccttgc gcttccccat accccggcgt ttgtccttat cgacggtccg
     9301 ctccgcctgg atctccccct tccgcaacgt ggcattgtca gcggggatca aaaatcggcc
     9361 tcggtcgcgg ccgcttcgat tgtcgcaaag gtgttccgtg accggtggat gaagcatctc
     9421 cacggtcttt atcccgctta tgcattccac gcgcacaagg gttatgggac cgccgagcat
     9481 ctggaagccc tgcaggcatt cggcccctgt cccgtccacc ggcgtagttt tgcgccggtc
     9541 gcgttctcgg aaaaagggcc gtttgaaaaa gctctcacat gaagatcgcc ccgaacccga
     9601 aaccgttccg ttattccgtc ggagatcgag gggaggtctg tgcggcggaa tatctcagga
     9661 aaaaaggttt taaggtcctt caggcgaact atcggtgcgc cctcggagag atcgatctcg
     9721 ttgccgaaaa ggcgggacgg attcattttg tggaggtgaa gacccggagc ggaaaaggat
     9781 tcggatcacc cgaggcagcc gtggatctcc ggaaacaggt gaagctcgtg aagcttgccg
     9841 cctggtacct caaggacaag aggctccagg accccccggt ctcttttgat gttatgggta
     9901 tcacgttgga ggagggcctc gcgcccgaga tcaggttctt tgagggtgcc tttgaaaaag
     9961 gagaggtgtg gtgagcggat ttttttcaag ggttttttcc cggcttgtca ggccctttgc
    10021 agaggtccat cccggggagc gcacaaagac cttcctgatg ttctgctatt ttctcctcac
    10081 gatttcgacg atctatatcc tgaagccggt ccgaagctct ctctttcttt ccgagttcag
    10141 cgccgacaag ctccgctacg tgaatatcgg agaagggctc ttccttatcg ccgtcgtctg
    10201 gctt
//
LOCUS       DAOOBX010000003         9037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_9502_length_9037_cov_4.483523, whole genome shotgun sequence.
ACCESSION   DAOOBX010000003 DAOOBX010000000
VERSION     DAOOBX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9502_length_9037_cov_4.483523"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLY30_00075"
     CDS             complement(<1..475)
                     /locus_tag="PLY30_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW64525.1"
                     /translation="MTPPLAIEPASRLQKLPPYLFAEIDRKKRELVAKGKDVIDLGVG
                     DPDIPTPDFIIRALQAAAENPRNHRYALDQGMPEFRQTIQEWFAKRFHVKLDPDKEIL
                     PLIGSKEGIGHLPLAIINPGQVCLIPDPGYPVYRSGTQLAGGEPFYMPLTEEGGYL"
     gene            complement(618..1409)
                     /locus_tag="PLY30_00080"
     CDS             complement(618..1409)
                     /locus_tag="PLY30_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64526.1"
                     /translation="MENKSAEKNTIGSGFLFFQLTNDERESAIYYLLEAILKARDSEP
                     DLNLPGYFDEDVNVYLAHLLFAMSLPEYHDMADPFLSDEPKEIVEWVRQTEDPMLKYF
                     IYKVNADNMLVRSTIFSEKPPEIKRVVFRKHGNGEGRLAVAYYDHAARCHKGIYQKRT
                     GVGEVLDKISDRFEVYQKILFRIKNDYFRFIDCFRDQAFQHFFLKMRQYERHARKDIV
                     LDQFLAAYHQWLVSRQPEVRGRVVSLVNELKAIDPGFCFDLTRLS"
     gene            complement(1571..2329)
                     /gene="recO"
                     /locus_tag="PLY30_00085"
     CDS             complement(1571..2329)
                     /gene="recO"
                     /locus_tag="PLY30_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPW64527.1"
                     /translation="MSIHKVEGIVLKTQPFRSSSLIVTFFTKEAGKIRGVVKGVHREG
                     EIRQAGFEIFTRAEIIFYEKRRSDLHLISDAAILESNDPIRNRLESIVYGSYFCELVD
                     ELTEVQDPHPEIFELLRTAFRFLSVVPPEKLTTVFTVKLLGAMGWIPYTDGCVSCGAR
                     PLEKGYFSVKQGALLCEACRSKDGGAPAITPVALALLRVFIRNEMRECLEHSHFSYAL
                     NEVQRLLTQFLNFRIGKVLRTKRFIDSVRSALVK"
     gene            complement(2337..2861)
                     /gene="ybeY"
                     /locus_tag="PLY30_00090"
     CDS             complement(2337..2861)
                     /gene="ybeY"
                     /locus_tag="PLY30_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPW64528.1"
                     /translation="MTMKKDAGKLSVQILNECPHPEVSESRLKKVAEKILRDLGFRQA
                     EVSILLVGDKKMRAMNRQFLRHAWSTDVLAFGRGKIVKITRVKEPVRQGKPGNFLGDI
                     ALCIPTIEGQAEEYGHTFIEELDYCLCHGILHLLGHKDKSREDHRRMHARQKQILGRV
                     LPRKVSPLRSGAGR"
     gene            complement(2858..5095)
                     /locus_tag="PLY30_00095"
     CDS             complement(2858..5095)
                     /locus_tag="PLY30_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPW64529.1"
                     /translation="MSAEKRRPSPEKVLPKTPSSRLIPAAILLASCVLACVILFWNRA
                     WDLERQEFMPGEPALRSYFSPVSFTYVDEVRTDASRLEKLKTVPPVYRIDPEMTAKTR
                     ARAESFFKILGEARIEKDGRGETRWKELPFRVPTSSLDTLLSAEDPEQLRLVTLAAID
                     AYLSRGILTETEKATLSRTNGGMVLLVSEDGQQEAVVSAERIVTLEDISKDAASALPS
                     EMFKKKGLRNATGDIIRGALLVNLGYDEVRTAELRKKEGASVPPALVMIKKNELIVQR
                     GMLVTDEIKQKLDQVLRKVSTRKQQVQIFTSSFLVLLLYVLTAIYFAAFEPAVFRSRS
                     LWLLFHTVVLLALVISKVVAVLPDASIYLMPTALAALLLTLLIHSRAGVMGGIMMTVM
                     SGFLSGFRMDLMIATLFASLAATFLSHRVRKRSHFLRIGLGIGGVFFLVIAGCQVMQN
                     TPWRDAAQFGLFGFLNALMTAVGSYLLLPFLEHFFDALTDVTLLELSDLNHPLIRKMM
                     VEAPGTYHHSLVVSSLAEDACEKIGAHSLLARVGCYFHDIGKLEKPEFFTENQGHLYA
                     NHHDVLPPRISFEIIINHVRDGVRIARKYRLKRAIVDFIREHQGTGVVYYFFKKAMDM
                     AAPAEQVLADDFRYPGPKPQTRETAVALLADSAEAASRSLVNPTPDSIQQLVRKIIND
                     KFIDGQLDECELTLRDLHKIQASFVRNLTAMFHTRMKYPLVENPLSAPDLFGTNQFSK
                     FRADK"
     gene            complement(5064..6026)
                     /locus_tag="PLY30_00100"
     CDS             complement(5064..6026)
                     /locus_tag="PLY30_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPW64530.1"
                     /translation="MLQKIIKIRSNDEAAAIYGNLDRNVRQAEKDFHVRLIAKNDRLK
                     IIGDKKDVEGAYDHFMRMKDNYRKGERNVERPAASDAKGTPPLRAEVSSKGAIHFFHR
                     GKVVQAKSENQLAYLKAIQSKDIVISIGPAGTGKTYLAMAAAVEALKKKYVGRIILTR
                     PAVEAGESLGFLPGDLAAKVNPYLRPLYDALYDMLEYEEVARYTERGAIEIAPIAYMR
                     GRTLNDSFVILDEAQNCTHSQMKMFLTRMGQTSKVVITGDITQVDLPAGKKSGLIEIR
                     QILSDIPDIQFIYLNETDVVRHPLVQEIIKAYEKHERRKAATES"
     gene            complement(6088..7878)
                     /gene="aspS"
                     /locus_tag="PLY30_00105"
     CDS             complement(6088..7878)
                     /gene="aspS"
                     /locus_tag="PLY30_00105"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPW64531.1"
                     /translation="MLRTVTCGELNKNSVGKTETLTGWVDTRRDHGNLIFVDLRDRWG
                     VTQVVFNPQTNATMHKTAETLRAEYVIRVKGKVEARPEGTVNAKIPTGEIELQAQEVE
                     VLNTCQPLPFEMGETNVNEELRLQYRFLDLRRRDMVESLTLRHRVTKLVHEYLDKQCF
                     IEVETPYLTKSTPEGARDFLVPSRLTHGAFYALPQSPQIFKQILMVSGYDRYYQFARC
                     FRDEDLRKDRQPEHTQIDLEMSFIDEEDIMGVVEGMVAHVFKHACGVDLKLPLRRMSY
                     KEAMEKYGSDKPDLRYGMEMKDLSGIFASSGFKVFDEVLAKKGSIRGLCFTPPAGVEF
                     SRKDFDDLTQWIKNFGAKGLAWFKVTGPETVESPIQKFFDAARIRKAIETTGAKAGDI
                     VFLVADTFKVACVALGALRCFLAEKHKMIPEGKFEMLWVVDFPLFEWNEEEKRCDACH
                     HPFTSPKPEDVPLLDTEPLKVRARAYDIICNGTEMGGGSIRIHDRKVQSKIFELLQLT
                     PEQQEAQFGFLLKALTFGAPPHGGLAIGLDRFVTLLLSRDSIREVIAFPKTQKGTCLM
                     TDAPSPATERQLRELNLDAKRMWIEKPNRT"
     gene            complement(7962..>9037)
                     /gene="hisS"
                     /locus_tag="PLY30_00110"
     CDS             complement(7962..>9037)
                     /gene="hisS"
                     /locus_tag="PLY30_00110"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPW64532.1"
                     /translation="DIVHKEMFSFKDRGERDMTMRPEMTASVARSVLEKGLLTQAKSL
                     RLYYIGPMFRAERPQAGRKRQFHQIGAEIVNEDHASDFDAIALAHGFLKYAGLKKFTL
                     NLNDLGDEESRARVRVALTDYFSKEKTKLCKDCQWRLDKNVLRIFDCKAHDCQPVIEK
                     APWEQFAPLSERFRTLAARLTEHGISYSINRRLVRGLDYYNGVVFEITAGGLGAQNAV
                     AGGGRYDNLYQDLGGAKTPCTGFSIGFERLLTALQAQDEKIFDELKTRRVYLAPLEDN
                     AAIATVIRDAALALRERGFRIETSPGVFSLSQHLKKADKVSARYALIAGAAELAKGVL
                     IVKDLAKREQSEVAFDQVSAFFK"
BASE COUNT         2003 a         2593 c         2465 g         1976 t
ORIGIN      
        1 gaagataacc gccttcctct gttaacggca tataaaaggg ttcccccccc gccagctggg
       61 tgccggaacg gtaaaccgga taacccggat cggggatgag acagacctgc ccgggattga
      121 tgatcgcgag cggcagatgg ccgatccctt ctttagaacc gatgagagga agaatctctt
      181 tatccgggtc cagtttgacg tgaaaacgtt tggcgaacca ttcctggatc gtctggcgga
      241 attccggcat cccctgatca agcgcgtacc ggtggttccg cggattctca gcggctgcct
      301 gaagcgcgcg aatgatgaaa tccggcgtcg ggatgtccgg gtcccccacg ccgaggtcga
      361 tcacgtcttt tcctttggcg acaagctccc gctttttccg gtcgatctcg gcaaaaagat
      421 aaggcggaag cttttgaaga cggctggcag gttcgatagc aagcggtggt gtcatcacag
      481 gatcctttcg atagcgaaga taacgcggct gagacttaaa aaccgagagc ggattatagc
      541 agactcggaa gaggttggaa atacacacaa agccggatca aggggactct gttacgcgaa
      601 aagagagcgg aaaacgccta ggacaacctg gtaaggtcga aacaaaaacc gggatcaata
      661 gccttcagct cattcacaag agaaaccact ctcccccgga cttccggctg acgagaaacg
      721 agccattgat gataagccgc aaggaactgg tcgagcacga tatcctttcg tgcgtgcctt
      781 tcatactgcc gcatcttgag gaagaaatgc tggaacgcct ggtcacggaa gcagtcaatg
      841 aaacggaagt agtcgttttt gatccggaac aggatctttt gataaacctc aaaccggtcc
      901 gaaatcttgt cgagaacttc gcccaccccg gtacgtttct gataaatccc cttgtgacac
      961 cgcgcagcat gatcgtaata ggccacggca agacggcctt ctccgttccc gtgctttctg
     1021 aaaacaacac gcttgatttc gggaggcttc tcggaaaaga tcgtggaccg gaccagcatg
     1081 ttatcggcat tcaccttata gatgaaatat ttcagcatcg ggtcttcggt ctgtcggacc
     1141 cattcaacga tctctttcgg ttcatccgaa aggaacggat cggccatatc atggtattcg
     1201 ggaagcgaca tcgcaaaaag aagatgcgcg aggtagacgt tcacatcctc atcaaaatag
     1261 ccggggaggt tgagatccgg ctcgctatca cgcgccttca ggatcgcctc caggagatag
     1321 taaatagccg attccctctc atcgttggta agctgaaaga aaaggaaccc gctgccaata
     1381 gtattttttt cggctgattt attttccata gtttatttca aaacataaca agcgcataca
     1441 actgattact tttgcgttct gctttaaaca taacataaca ttattatccc gtcaaatctc
     1501 acacacttgt tgacaatatc ataatctatt gcaaacaaac aacttgcgga tcttgttata
     1561 ggaatgagta ttattttacg agggcagaac gaaccgaatc aatgaaccgc tttgttcgga
     1621 gaacttttcc gatacgaaaa ttgaggaatt gggtaagcag gcgctgcacc tcgttcaagg
     1681 cgtaagagaa atgagaatgc tcaagacatt ccctcatctc gttacggatg aacacacgca
     1741 gcaaggcgag tgcgacggga gtgatcgcgg gagctcctcc gtctttcgag cggcaggctt
     1801 cgcacaaaag ggcgccctgt tttacggaaa aatagccttt ttcgagaggt cgggcaccgc
     1861 acgaaacaca gccgtccgtg tacgggatcc agcccatcgc tccgaggagt ttaacggtga
     1921 acacggtcgt cagtttctcc ggagggacca ctgacaagaa cctgaaggca gtccgaagca
     1981 gttcgaagat ctccggatgc gggtcctgta cttcggtgag ttcgtccaca agctcgcaga
     2041 aataactccc gtagacaatg ctttcaagac ggttacggat cgggtcgttg ctttcgagga
     2101 tcgcggcatc ggagatcaaa tggaggtccg agcgcctctt ctcgtagaaa atgatctcgg
     2161 ccctcgtaaa aatctcgaat ccggcttgcc ggatctcccc ttcgcggtga acgcctttga
     2221 cgaccccgcg aatcttcccg gcctcttttg taaagaaggt aacgatcagc gagctcgacc
     2281 ggaacggctg ggtcttgaga acgatcccct caaccttgtg gatgctcatg aaaggatcag
     2341 cgtcccgcgc cggaacggag aggagagacc tttcggggaa ggacccggcc gagaatctgc
     2401 ttttgccttg catgcatgcg ccggtgatct tcgcgggatt tatccttgtg tcccagcaag
     2461 tggaggatgc cgtggcaaag acagtaatca agctcctcga tgaacgtatg cccgtattcc
     2521 tcggcctgcc cttcgatcgt cgggatacaa agcgcgatat ctcccaggaa attccctggc
     2581 ttcccttgcc gcacgggttc cttgacccgg gtgatcttga cgatcttgcc ccgaccgaac
     2641 gcgaggacat ccgtcgacca ggcgtgcctc aggaactgcc ggttcatcgc ccgcatcttc
     2701 ttatctccca cgagaaggat gctgacctcg gcctgcctga aaccgaggtc gcgcaggatt
     2761 ttttccgcga ccttttttaa ccgggattcg gaaacctccg gatgcgggca ttcgttcagt
     2821 atctggaccg acagtttccc ggcatccttc ttcatggtca tttgtctgcc cggaacttcg
     2881 aaaactggtt ggtgccgaaa agatcgggag ccgaaagcgg gttttccacc aacggatatt
     2941 tcatgcgcgt gtggaacatg gcggtcaggt tcctgacaaa gctcgcctgg attttgtgga
     3001 gatcccgcag cgtcagctca cattcatcga gctgaccgtc gataaacttg tcattgatga
     3061 tcttgcgaac aagctgctgg atcgagtccg gcgtgggatt cacgagagat cgggatgccg
     3121 cttcggcgga atccgcaagg agcgccacgg ccgtctcccg ggtttgcggc ttcggacccg
     3181 gatagcggaa atcgtccgcc aggacctgct ctgccggagc ggccatatcc atcgcttttt
     3241 tgaaaaaata ataaaccacg ccggtcccct gatgctcacg gataaaatcc acgatcgccc
     3301 gcttcaaacg gtatttgcgg gcgatccgga cgccgtcccg gacatggttg atgatgatct
     3361 cgaagctgat acgcggcgga aggacgtcgt ggtgattcgc gtataggtgt ccctggtttt
     3421 cggtaaaaaa ctccggtttc tcgagcttgc cgatgtcgtg gaaatagcaa ccgaccctcg
     3481 caaggagcga atgcgccccg attttttcgc aggcgtcctc ggcaagcgaa ctcacgacca
     3541 gactgtggtg ataggtcccg ggcgcctcca ccatcatttt ccgtatcagc ggatgattga
     3601 ggtccgacag ttcgagcagc gtcacatccg tcagggcatc gaaaaaatgt tccaggaaag
     3661 gcagaagcag atagctcccc accgcggtca tcagcgcgtt caggaatccg aaaagcccga
     3721 actgcgcggc atcccgccat ggcgtatttt gcatcacttg gcaacccgcg atcaccagga
     3781 aaaagacgcc tccgatgccg agcccgatcc tcagaaaatg agatcgcttg cggacccggt
     3841 gcgacaggaa ggtcgccgca agggacgcga acagggtcgc gatcatcaaa tccatacgga
     3901 aaccggaaag aaaaccgctc attaccgtca tcatgatccc gcccatgacc ccggcgcggc
     3961 tgtgaatcaa caaggtcagg aggagcgccg ccagagccgt cggcatcaga tagatcgaag
     4021 catccggcag taccgcgaca accttggaaa tgaccagcgc caggaggacc accgtgtgga
     4081 acaggagcca aagggaacgg gagcggaaaa ccgcgggttc aaaagccgcg aaatagatcg
     4141 ccgtgaggac gtaaagaagg agaaccagga acgagctcgt aaagatctga acctgctgct
     4201 tgcgcgtgga aaccttgcgc aggacctggt ccagtttctg cttgatctcg tcggtcacca
     4261 gcattccccg ctggacgatc agctcgttct tcttgatcat gaccagggcc ggaggaacgg
     4321 acgccccctc ttttttcctg agttccgccg tccggacctc gtcataaccc aggttcacga
     4381 gaagcgcgcc gcggatgata tcgccggtcg cattccggag ccctttcttt ttgaacattt
     4441 cggaaggaag ggccgacgcg gcatccttcg agatatcctc cagcgtcact atccgctccg
     4501 ccgacacaac cgcttcctgc tgcccgtctt cggacacaag aaggaccatt ccgccgttgg
     4561 tgcgggaaag ggtcgccttc tccgtttccg taagaatccc gcgggaaaga taggcgtcta
     4621 tggcagcaag ggtcaccagg cgcagctgct cgggatcttc tgccgataac aatgtgtcga
     4681 gcgatgaagt cggaacacgg aacggaagtt ctttccagcg cgtctctccc cttccgtctt
     4741 tttcgatccg ggcttccccg aggatcttaa aaaaactctc cgcccgggcg cgagtcttcg
     4801 cggtcatctc cggatcgata cggtagaccg gagggacggt cttcagtttt tcgagacggc
     4861 ttgcatcagt cctcacttca tcgacatagg tgaaagagac cggggagaaa taagaccgca
     4921 gggccggctc tccgggcatg aactcctgac gttcgaggtc ccaggcccgg ttccaaaaaa
     4981 gaatgacaca ggccaggaca cacgacgcta aaaggatcgc cgcgggaatc aaccgcgaag
     5041 acggggtctt gggaaggact ttttcaggac tcggtcgccg cttttctgcg ctcatgtttt
     5101 tcataggcct tgatgatctc ctgcaccaag ggatgacgga ccacgtcggt ttcgttcaga
     5161 tagataaact ggatatcggg aatgtccgag agaatctgcc ggatctcgat caggccggat
     5221 ttcttcccgg ccgggagatc gacctgcgtg atatcgcccg tgatcacgac cttggaggtc
     5281 tgtcccatac gcgtcaggaa catcttcatc tgactgtgcg tgcagttctg ggcctcgtcc
     5341 aggatcacga acgaatcgtt caacgtccgg cctctcatgt aggcgatcgg ggcgatctcg
     5401 atcgccccgc gctcggtgta gcgggcgacc tcttcgtact caagcatgtc ataaagagca
     5461 tcatacagag ggcggagata gggattcact ttcgcggcaa gatcgcccgg aaggaatccg
     5521 agactctccc cggcctcgac cgcgggacgg gtcaggatga tccttccgac gtacttcttc
     5581 ttcaaggctt cgaccgcggc cgccatcgcg aggtacgttt tgccggtacc ggcagggccg
     5641 atcgagatca cgatatcttt cgactggatg gccttgaggt aggcgagctg gttctcgctc
     5701 ttcgcctgaa ccactttccc gcgatggaaa aaatgaatcg cgcccttcga cgacacctca
     5761 gccctgagag gcggcgttcc tttcgcatcc gacgcggccg ggcgttccac gttccgctcc
     5821 cctttccggt aattgtcctt catcctcatg aaatgatcgt aggcgccctc gacgtccttc
     5881 ttgtcgccga tgatcttcag ccggtcattc ttcgcgatca gccggacatg aaaatccttt
     5941 tccgcctggc ggacattccg gtccagattg ccgtagattg ccgccgcttc atcgttcgaa
     6001 cggatcttga tgatcttttg caagatgtaa tcctttcgtt agggaacagg agatagggtt
     6061 tggttctcta tttcaatact ctatacgcta tgtcctgttt ggtttttcaa tccacatccg
     6121 tttcgcatcg agattcagtt ccctgagttg ccgctccgtc gcgggcgacg gcgcgtccgt
     6181 catcagacag gttcctttct gcgtcttcgg aaacgctatc acctcgcgga tcgaatcccg
     6241 ggaaagcaga agcgtcacga accggtcaag accgatcgcg agccccccgt gcggaggcgc
     6301 gccgaaggtc agggctttca ggaggaaacc gaattgcgct tcctgctgct ccggcgtcaa
     6361 ctgcagaagc tcgaatattt tcgactgcac tttacggtca tggatacgga tgcttccgcc
     6421 ccccatttcc gtgccattac agatgatgtc atacgcccgc gcgcggacct tgagcggctc
     6481 cgtatcgagc agcggaacat cttcgggctt cggcgacgtg aacggatggt ggcacgcatc
     6541 gcagcgtttt tcttcctcgt tccattcaaa gagcgggaaa tcaacgaccc agagcatctc
     6601 gaatttcccc tcggggatca tcttgtgttt ttccgcgaga aagcagcgaa gcgcgccaag
     6661 cgcgacacaa gcgaccttga acgtgtccgc aacgaggaac acgatgtccc ccgccttcgc
     6721 gcctgtcgtc tcgatcgcct ttctgatccg cgcggcgtcg aagaacttct ggatcggcga
     6781 ttcgaccgtc tccggcccgg tcaccttgaa ccacgcgagc ccctttgcgc cgaaattttt
     6841 gatccactgc gtgagatcat cgaaatcctt ccgggaaaac tcaactccgg caggcggtgt
     6901 aaagcagagg ccgcggatcg accccttctt cgccagcact tcatcgaaca ccttgaaccc
     6961 gctcgatgca aagattccgg agagatcctt catctccatc ccgtagcgaa ggtctggctt
     7021 gtccgaaccg tatttctcca tcgcttcctt gtagctcatg cggcggagcg ggagcttgag
     7081 gtcgaccccg cacgcatgtt taaaaacatg cgcaaccatt ccttcaacca cccccatgat
     7141 gtcttcttcg tcgatgaagg acatttcgag gtcgatctgc gtgtgctcgg gctggcgatc
     7201 cttacggagg tcctcgtcgc ggaaacagcg cgcgaactga taatagcggt catagcccga
     7261 gaccatcagg atctgcttga aaatctgcgg ggactgcgga agcgcgtaaa aggcgccgtg
     7321 cgtgaggcgc gagggcacga gaaaatcgcg tgcgccctcg ggggtgctct tcgtcagata
     7381 cggcgtttcg acctcgataa aacactgttt gtcgagatac tcgtgcacga gtttcgttac
     7441 gcggtggcgg agcgtcaggc tctccaccat atcgcggcgg cgcaggtcga ggaaacggta
     7501 ttggagccgg agctcttcat taacgttcgt ctcgcccatt tcgaaaggca agggctggca
     7561 ggtgttcaga acctcgacct cctgggcctg aagctcgatc tcgcccgtcg gaatcttcgc
     7621 gttcacggtc ccctccggcc gggcctcgac cttccccttg acgcggatca catactccgc
     7681 gcgaagcgtc tccgccgtct tgtgcatcgt ggcgttcgtc tgcggattga aaacgacctg
     7741 tgtcacgccc caacggtcgc ggagatccac aaagatcaga ttcccgtgat cgcggcgggt
     7801 gtccacccag cctgtcaggg tctccgtctt cccaaccgaa tttttgttca attcaccgca
     7861 ggtcactgtc cttagcataa atcaatcacc tttcgacgtt tccccccggg agcagggaga
     7921 tttaaatccc ccggctcccc tttttgcaaa gggggaccat gttatttaaa aaacgcggac
     7981 acctgatcga acgcgacttc cgactgttcc cgtttcgcga ggtccttgac gatcagcacg
     8041 cctttcgcca gctcggccgc acccgcgatc aaggcgtagc gcgccgagac cttgtccgct
     8101 tttttgagat gctggctgag cgaaaaaacg ccgggcgagg tttcaatccg gaatccccgt
     8161 tcgcggagcg ccaaggcagc gtcacggatc accgtggcga tcgcggcgtt gtcttcgagc
     8221 ggtgccagat agacacgcct cgttttcagt tcatcaaaga ttttttcatc ctgggcctgc
     8281 aaagccgtga gcaaccgttc gaaaccgatg ctgaaccccg tgcagggcgt cttcgcgccg
     8341 ccaaggtcct gataaaggtt gtcatagcgc ccgccgcccg caacggcgtt ctgggcgccg
     8401 agcccgcccg ctgtgatctc gaaaacaaca ccgttgtaat aatccaggcc gcgcacgagc
     8461 ctccggttga tcgagtacga aataccgtgc tcagtgagcc tggccgcaag cgtccggaac
     8521 cgttcggaaa gcggggcgaa ctgttcccag ggggctttct cgatcacggg ctgacagtca
     8581 tgcgctttgc agtcaaagat cctgagaaca ttcttatcga gccgccactg gcaatccttg
     8641 caaagttttg tcttttcttt tgagaaataa tccgtgaggg ccacgcggac cctcgcgcgg
     8701 ctttcctcat cgccgagatc attcaggttc agggtaaatt tcttcaggcc ggcatacttg
     8761 agaaaaccat gggcaagggc gatcgcgtca aagtccgagg catgatcctc gttgacgatc
     8821 tcggccccga tctggtggaa ctggcgtttg cggcccgcct gggggcgttc ggcgcggaac
     8881 atcgggccga tataataaag acggagagat ttcgcctggg tcaggaggcc cttctcaagc
     8941 accgaacgcg cgacggaggc ggtcatctcg ggacgcatcg tcatgtcccg ctcaccacgg
     9001 tctttaaaag agaacatctc cttatggacg atgtcgc
//
LOCUS       DAOOBX010000004         6704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_13503_length_6704_cov_5.521582, whole genome shotgun sequence.
ACCESSION   DAOOBX010000004 DAOOBX010000000
VERSION     DAOOBX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13503_length_6704_cov_5.521582"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PLY30_00115"
     CDS             complement(<1..1133)
                     /locus_tag="PLY30_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HPW64533.1"
                     /translation="MASKILKNPFVILLSIALGVAFGFYNKGLAIQLGVIGDMYLSFF
                     QMSVIPILITAIVSSLAQLMKDKNVQKYLIRIVFVFLAVLALTAAFGTALGLVGKPGS
                     DLGDKTTATLNRIIKTSSQNQDQEMALRNPAGDSVAKKTSLFDFFLHIIPSNIFTSLS
                     SGRILEIVFFSIIFGIAVGFLKDSSSNFLLDLSKSLLEAFQKLINWTMYGLPFGLFFL
                     IGKQIAEVGVDIFVAMIKFIVVFYAGGGILFLACTIIVWVRSGERNPFKVLHELIDPI
                     VISFATRNSFASLPASIQSLTALRFDPKTTNLVFPLGLTILRFGNILYFALGACFVAQ
                     IYGAVLPTQAILVIVVGSLFAGTATAGSSGLLTLPMISLILDPL"
     gene            complement(1145..1873)
                     /locus_tag="PLY30_00120"
     CDS             complement(1145..1873)
                     /locus_tag="PLY30_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW64534.1"
                     /translation="MKAILSEKTLVVAMTATDQPPFYFINDEGNLVGFDVDIARKMAK
                     ELGITRLVINREAKSFNDVVSMVASGKADLAISKLSRTLMRARYVKFSTPYIVLTQGL
                     LFNRLQLARYTSEENVRSFIRGFEGKIGVIQSSSYVNYAKENFPKAQIVEYPTWDAVV
                     AAVVAGDVLAAYRDDLEIRKIMDSLPNSALTLKPMYFTDLTDPIAIAVKSDNTQLLSW
                     VNTFLEIQAYNLTSDELIKKYLIK"
     gene            complement(2061..2516)
                     /gene="dtd"
                     /locus_tag="PLY30_00125"
     CDS             complement(2061..2516)
                     /gene="dtd"
                     /locus_tag="PLY30_00125"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPW64535.1"
                     /translation="MRAVIQRVARSSVSVDGAIVSETGRGFMVLLGITEGDGEKDIAY
                     ICDKLLNVRVFPDDNYKMNLSLSDIGGDILIVSQFTLYADARNGRRPDYALAAKGDIA
                     RPVYESVVTRLKESYVPDRIKTGVFGAMMEVSLVNEGPVTILLDSNKTF"
     gene            2586..4226
                     /locus_tag="PLY30_00130"
     CDS             2586..4226
                     /locus_tag="PLY30_00130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPW64536.1"
                     /translation="MHRMKVDTTSGCGDNSVFTDASAVADPVAEMATSAFGIKYLFPW
                     QRLVIANILDAVQASNDARDTPDARNAVAGDTPDILAGDELHDEDGALRGQQIVLLPT
                     GAGKSLCFQIPALFLDKPTLVIYPLLALMSDQMRRMNESALEAVIFRGGQSPEERKAQ
                     FARLDGSDGKPPARLVIANPEVLVASEAVIGRLEKRGISHFAIDEAHCVAEWGDSFRP
                     SYLELGGVIERLRPSAVTAFTATASPPVLARIADALFGGQAHVVRGDTDRPNIKYRVV
                     ACRAKAPALVREVASRERPMVIFCATRGGTEKTARMLREILHDEDILFYHAGLRKEEK
                     QAVEAWFHGHARAILCCTCAWGMGVDKKDIRTVIHKDAPPTVEAYVQEAGRGGRDGLD
                     AEAVLLWSMDDATKIAKKADRSSIFLKYAECSGCRRDVILRALGDPGAGADAPEGKTV
                     ACSGCDVCDGTAIRHERDAEIVVEAVRKNSHAYTKAELAEYLYERGNALSRERHGFAQ
                     WRLADFSLMIDSLLKDGSLRELDRWPFRGKLALTPVRG"
     gene            complement(4262..4591)
                     /locus_tag="PLY30_00135"
     CDS             complement(4262..4591)
                     /locus_tag="PLY30_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64537.1"
                     /translation="MDISRLLELLKRVLLTPEVIGVTIVMIVFFSLVNFVVYYRKRPP
                     TPKPKKVEAAPVAAEGASKSGGKGAGRPSDEDEDEEEEEADAKKKKGGKRGKKADKGT
                     KESAEEE"
     gene            4658..5338
                     /gene="hisH"
                     /locus_tag="PLY30_00140"
     CDS             4658..5338
                     /gene="hisH"
                     /locus_tag="PLY30_00140"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPW64538.1"
                     /translation="MTGIVDYNAGNIRSVELALQSLGVSYRISKRPAELEGMDRVIFP
                     GVGEARFAMKELRSSGFDGFLRDWAASGKPLLGVCLGSQIIFGHSEENDTPCLGIVPG
                     SVRRFPADFRARSLKVPHMGWNDIRYRNGGSPIFDGVREGSDFYFVHSYYVDPADPSV
                     VSATANYGIDFACAVKMGNVEAVQFHPEKSGSAGLAVLSNFCRADIANNADIAGAGIA
                     GAKGESRA"
     gene            5331..6095
                     /gene="hisF"
                     /locus_tag="PLY30_00145"
     CDS             5331..6095
                     /gene="hisF"
                     /locus_tag="PLY30_00145"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPW64539.1"
                     /translation="MLKKRIIVCLDVKDGRTTKGVKFLDNVDIGDPVVMATEYYRQGV
                     DELVFYDIMASARDRGPTLELIARVAEAIHIPFCVGGGISSVDDIRNTILAGAEKVSI
                     NSPAVRNPELLTAGANIFGAQCIVLGMDAKRDASMKSGYRVFVNGGRVATELDALEWA
                     IRAESLGAGEIVLNSIDADGTRDGYEINLTRMISENVGIPVVASGGAGTVDHMARVLG
                     EGKADAALIASMVHSGDYTIGAIKDALASKGVPVRR"
     gene            complement(6102..6620)
                     /locus_tag="PLY30_00150"
     CDS             complement(6102..6620)
                     /locus_tag="PLY30_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64540.1"
                     /translation="MEENIVNAILLWTGYKNAEGLRDKAVWTTPREGITRIEATGTAK
                     DCTFIVAKAGDPASWQIAAPESAKSVALDGTKADSFALLFSSIVADSYASDGTTLPET
                     PVARFVVQGTGKQYTLTIGSKVGGSGDKAADANNAADAKYLCSSSETPYAFYMTASIT
                     ERLLAPYTSLKK"
BASE COUNT         1390 a         1932 c         2050 g         1332 t
ORIGIN      
        1 agcgggtcga ggatgaggct gatcatcggg agggtcagga ggccggagga gcccgcggtc
       61 gccgttcccg cgaagagcga gccgacgacg atgacgagga tcgcctgcgt cggaaggacg
      121 gcgccgtaaa tttgcgcgac gaagcacgcg ccgagcgcga aatacaggat gttgccgaaa
      181 cgaagaatcg tcaggccgag cggaaagacg aggttcgtcg tcttcgggtc aaaccggagc
      241 gcggtaagcg actggatgga agccggcaac gaggcgaaac tgtttctcgt cgcgaacgag
      301 atgacgatcg ggtcgatgag ctcatgcagg accttgaacg ggttccgctc gcccgagcgt
      361 acccacacga tgatagtgca cgcgagaaag agaattcccc cgcccgcgta gaacacgacg
      421 atgaacttga tcatcgcgac aaatatatcg acgccgacct ccgcgatctg cttgccgatg
      481 aggaagaaaa gcccgaaggg aaggccgtac atcgtccagt tgatgagttt ctggaacgcc
      541 tcgagaaggg attttgacag atcaagcagg aaattcgacg agctgtcttt caggaagccc
      601 accgcgatgc cgaatataat gctgaaaaat acgatctcga gaatgcgtcc cgaactcagg
      661 gacgtgaaaa tgttcgacgg aatgatatgc aggaaaaagt cgaaaagaga ggtttttttc
      721 gcgaccgagt ctccggccgg attgcggagg gccatttcct ggtcctggtt ctggctcgac
      781 gtcttgatga tccggttcaa cgtcgccgtc gtcttgtcgc caaggtcgga gccgggcttg
      841 cccacgaggc cgagcgcggt accgaaggcc gcggtcaacg cgagcacggc cagaaagacg
      901 aatacgatgc ggatgaggta tttctgtacg ttcttgtcct tcatgagctg ggcgaggctc
      961 gaaacgatcg ccgtgatgag gatcgggatg acgctcatct ggaaaaagct caggtacatg
     1021 tcgccgatca cgccgagttg gatcgcgagt cccttgttat agaagccgaa cgcgacgccg
     1081 agcgcgatcg agagcaatat gacaaacggg tttttcagaa ttttggaagc catcggaaat
     1141 ctccttattt aatcaggtat tttttgatca attcgtcgga cgtgaggtta tacgcctgga
     1201 tctcgaggaa cgtattcacc cagctcagga gctgcgtatt gtccgacttg accgcgatgg
     1261 cgatcgggtc cgtaaggtcc gtgaagtaca tcggtttgag cgtaagggcg ctgttcggaa
     1321 gcgaatccat gatcttgcgg atctccaggt cgtcgcgata cgccgcgagg acatcgcccg
     1381 cgacgacggc cgcaacgacg gcgtcccagg tcgggtactc gacgatctgc gccttcggga
     1441 aattttcttt cgcgtaattt acataggacg aactctggat gacgccgatc ttgccctcga
     1501 agcccctgat gaaactccgg acgttttcct ccgacgtata ccgggcaagt tgaagacggt
     1561 taaaaagaag accctgcgta aggacgatgt acggcgtcga gaacttgacg taccgggccc
     1621 gcatgagcgt cctgctcagt tttgatatcg cgaggtcagc cttccccgac gcgaccatcg
     1681 aaacgacgtc gttgaaagat ttcgcctcgc gattgatgac caggcgggta atccccagtt
     1741 cttttgccat cttgcgcgcg atatcgacgt cgaaaccgac caggttccct tcatcattga
     1801 taaaatagaa aggaggctgg tcggttgcgg tcatggcgac gacaagggtt ttttccgata
     1861 aaatcgcctt catgtcggcg ggaatgtcct tgtcttcgac gatttgagaa cacagaaaca
     1921 tcgacacgac acacaggaac actccgactg atgcgaattt cttcactttt ttccctccga
     1981 aaataaactc caatagagta attctatcat ttatttcctt attatcggag aaaagtttca
     2041 aaaaacaggg aaaattttca ttaaaacgtc ttgttcgagt cgaggaggat cgttacgggg
     2101 ccctcattaa cgagcgaaac ttccatcatg gcgccaaaaa cgccggtctt gatccggtcg
     2161 ggaacatacg actccttgag ccgcgtgacc acgctttcgt agacggggcg cgcgatgtcg
     2221 cccttcgcgg cgagcgcata atcgggacgc ctgccgttcc gcgcgtccgc atacaacgtg
     2281 aattgagaga ctatcaggat atcccccccg atgtcggaga ggctcaggtt catcttgtag
     2341 ttgtcgtcgg gaaacactcg cacgttcagg agcttgtcgc agatatacgc gatgtccttt
     2401 tccccgtcgc cctcggtaat gcccaaaaga accatgaacc cacgccccgt ttcggaaacg
     2461 atcgccccat cgacggagac cgatgatcgc gctacacgtt gtatgactgc ccgcatgctc
     2521 ccgtagtagc ccattcatcg cgcgatgtca agcaaatccg ggcaaacaaa tacattgcgg
     2581 actatatgca tcgcatgaag gtcgatacta caagcggctg cggcgacaac agtgtgttta
     2641 ccgatgcgtc agcagtcgcc gatcccgtcg cggaaatggc gacctccgcg ttcggcataa
     2701 aatatctctt tccctggcag agactcgtga tcgcgaacat cctcgatgcc gtgcaagcgt
     2761 cgaacgacgc gcgtgacaca ccggacgcga ggaacgcggt tgccggggac acgccggaca
     2821 tacttgccgg cgacgaactc cacgacgagg acggcgcgct tcggggacag caaatcgtgc
     2881 ttctcccgac gggcgcggga aaatccctgt gcttccagat accggcgctt ttcctcgaca
     2941 agccgacgct cgtgatatat ccattgctcg cgttgatgag cgatcaaatg cgccgcatga
     3001 acgagagcgc gctcgaggcg gtcatctttc gcggcggaca aagcccggag gagcggaagg
     3061 cgcaattcgc gcggcttgac gggagcgacg gaaagccgcc ggcgcggctc gtcatcgcga
     3121 acccggaagt cctggtcgcg tcggaggcgg tcatcggccg actcgaaaaa cgcggcatct
     3181 cgcacttcgc gatcgacgag gcgcactgcg tcgccgagtg gggcgacagt ttccggccgt
     3241 cgtatctcga gttgggcgga gtcatcgaac ggctcaggcc gagcgccgtc accgcgttca
     3301 ccgcgacggc ctcccctccc gtgctcgcgc gcatcgcgga cgccctcttc ggcgggcagg
     3361 cgcacgtcgt gcggggcgac acggatcggc cgaacatcaa gtaccgcgtt gtcgcatgcc
     3421 gggcaaaggc gccggctctc gttcgcgagg tcgcttcgcg cgagcggccc atggttatct
     3481 tctgcgcgac gcgcggcggc acggaaaaga ccgcgcggat gctgcgtgaa attctccatg
     3541 acgaagatat cctcttttac cacgcgggtc tccggaagga agaaaaacaa gcggtcgagg
     3601 catggttcca tgggcacgcg cgggcaatac tgtgctgcac atgcgcatgg ggaatgggcg
     3661 tcgataaaaa agacatcagg acggtgatac acaaagacgc gcctccgacg gtcgaggcgt
     3721 acgtgcagga agcgggccgg ggcgggcgcg acgggctcga cgcggaggcg gtacttctat
     3781 ggagcatgga cgacgcgacg aaaatcgcga aaaaggcgga ccggtcatcg atatttctga
     3841 aatacgcgga gtgttccgga tgccgacggg acgtgatcct tcgcgcgctc ggggaccccg
     3901 gggccggagc ggacgccccc gaagggaaaa ccgtcgcgtg ctcgggatgc gacgtatgcg
     3961 acgggacggc cattcgtcat gaacgcgacg ccgaaatcgt cgttgaggcg gtacgaaaga
     4021 acagccatgc gtacacgaag gccgagctcg ccgagtattt gtatgagcgg ggaaacgcgc
     4081 tgtcgaggga acgtcacggg ttcgcgcaat ggcggctcgc cgacttttcg ctgatgatcg
     4141 actcgctttt gaaagatggt tctctcaggg aactggatcg atggcctttc aggggaaaac
     4201 ttgccttgac gccggtacgc gggtaagcgc gggtagtcgc gggtcatcac gggtagtcgg
     4261 tctactcttc ctcggccgat tccttcgtgc ccttgtcggc ttttttcccg cgctttccac
     4321 ccttcttctt tttggcgtct gcctcttctt cttcctcatc ctcgtcttcg tcgctcggtc
     4381 gtccggcgcc tttaccgcct gatttcgatg cgccttcggc cgcgacggga gcggcctcta
     4441 cttttttcgg cttgggcgtc ggcggtcgtt tccggtaata cacgacgaaa ttgacaaggc
     4501 tgaaaaaaac gatcatgacg atcgtgacgc cgatgacttc gggggtcagg agaacgcgtt
     4561 tgaggagttc gagcagccgg gaaatatcca taagccgatt atcggcacga gcgggctgcg
     4621 ccttgaccag cgtaagggcg gcgggtagga tgatttcatg acaggaatag ttgactacaa
     4681 cgcgggaaat atccgcagcg tggaactcgc gctccagtcg ctcggcgtct cgtaccggat
     4741 ctcgaaacgg ccggcggagc tcgagggcat ggaccgggtc attttcccgg gcgtcggcga
     4801 ggcgcggttc gcgatgaagg aattgcgttc gtcgggcttt gacggatttc tccgcgattg
     4861 ggccgcttcg ggcaagcccc ttctcggcgt gtgtctcggt tcgcagatca ttttcgggca
     4921 ttcggaggaa aacgacacgc catgcctcgg catcgtgccg ggtagcgttc gccgctttcc
     4981 cgccgacttc cgcgcgcgat cactcaaggt cccgcacatg ggctggaacg acatccggta
     5041 ccggaatggc ggctcgccga tattcgacgg cgttcgcgag ggctcggact tttatttcgt
     5101 gcattcctac tacgtcgatc ccgcggatcc gtcggtcgtc tcggcgacgg ctaactatgg
     5161 catcgacttc gcgtgcgccg tcaagatggg caacgtcgag gcggtgcagt tccaccccga
     5221 aaaatcaggt tccgcaggac tcgcggtcct ctcgaacttt tgccgcgccg atatcgcgaa
     5281 caacgccgac atcgcgggcg ctggcatcgc gggcgcgaag ggggaaagcc gtgcttaaga
     5341 aacggatcat cgtctgcctc gacgtcaagg acggccggac gaccaagggc gtgaagtttc
     5401 tcgacaacgt cgacatcggc gacccggtcg tcatggccac cgaatattac cggcagggcg
     5461 tcgacgagct cgtgttctac gacatcatgg cgtcggcgcg cgaccggggc ccgacgctcg
     5521 agctcatcgc gcgcgtcgcc gaggcgatcc acataccgtt ctgcgttggc gggggcatct
     5581 cgtcggtcga tgacatcaga aacaccatcc tcgccggcgc cgagaaagtc agcatcaact
     5641 cgccggccgt gcgaaatccg gagcttctga ccgcgggcgc gaacatcttc ggagcgcagt
     5701 gcatcgttct cggcatggac gccaagcggg acgcatcgat gaaatcggga taccgcgtat
     5761 tcgtaaacgg cggccgcgtc gcgaccgaac ttgacgcgct tgagtgggca atccgggcgg
     5821 aatcgctcgg cgcgggggaa atcgtcctca actcgataga cgcggacgga acgcgcgacg
     5881 gctacgagat caacctcacg aggatgatca gcgaaaacgt cggcattccc gtcgtcgcct
     5941 cgggcggcgc gggcacggtg gaccacatgg cccgcgtgct cggcgagggc aaggctgacg
     6001 ccgctctcat cgcgtcgatg gtacattccg gcgactacac gatcggcgcg atcaaggacg
     6061 ccctcgcctc gaagggcgtc ccggtgagaa gatagcgcgc gttatttctt gagcgacgtg
     6121 tacggcgcga ggagccgctc ggtaatcgac gcggtcatgt agaacgcgta cggcgtttca
     6181 gacgacgagc acaggtactt cgcgtctgcg gcgttattcg cgtcagccgc cttgtccccg
     6241 ctcccgccga ccttcgatcc gatcgtcaga gtatactgtt tccccgttcc ctgcacgacg
     6301 aagcgggcga ccggggtttc cggaagcgtc gtcccgtcgc ttgcgtacga atccgcgacg
     6361 atcgacgaga acaagagggc gaacgagtcg gccttcgtgc cgtccagcgc gacagacttc
     6421 gccgactcgg gagcggctat ctgccacgac gcgggatctc ccgccttcgc gacgatgaac
     6481 gtgcaatcct tcgccgttcc cgtcgcctcg atccgggtga tgccctcgcg cggggtcgtc
     6541 cagacggcct tgtccctgag tccttccgcg tttttatatc cagtccataa taatattgca
     6601 tttacaatgt tctcttccat ttattcctcc gcccgcgcag cgtccatcca acatttgctt
     6661 aacctgtggt ttcgtgcccg aagggcttgg cgcattttgt gcgt
//
LOCUS       DAOOBX010000005         6657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_13625_length_6657_cov_3.757195, whole genome shotgun sequence.
ACCESSION   DAOOBX010000005 DAOOBX010000000
VERSION     DAOOBX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13625_length_6657_cov_3.757195"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..634)
                     /gene="ruvC"
                     /locus_tag="PLY30_00155"
     CDS             complement(134..634)
                     /gene="ruvC"
                     /locus_tag="PLY30_00155"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPW64541.1"
                     /translation="MRVMGVDPGTHRMGFGVLETSGRDVKVVICGTIRANSRDPIAKR
                     LLHMFESLQKNIQSYKPDALAIESLFFAKDVRAVERIGEARACAMLAASKEGIEVIEY
                     APTAVKRSVTGNGRGSKEQVQFMVKRLLNLKEAPALDASDALAIALCHAHSARQVKCI
                     NTLQVK"
     gene            complement(681..1430)
                     /locus_tag="PLY30_00160"
     CDS             complement(681..1430)
                     /locus_tag="PLY30_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW64542.1"
                     /translation="MSGHNKWASIKHKKAAADAKRGAAFTKIIREITVAAKTGGGDPS
                     MNPRLRTAIQAGKDANMPADNIERAIKKGTGELEGVTYEEVTYEGFGPGGAAFLVACL
                     TDNKNRSASEIRSIFGKNGGNMGSAGGVAWMFEKKGLIVVSTSTTTEDKLMETALNAG
                     AEDMAAVGETFQVTTAPADLWTVRDAIEKSGIKIESANLTMNAKNMTTVDVAAGRTVV
                     KLIEALEEHDDVQNVYTNADIPDEVMKEITG"
     gene            complement(1544..2377)
                     /gene="folP"
                     /locus_tag="PLY30_00165"
     CDS             complement(1544..2377)
                     /gene="folP"
                     /locus_tag="PLY30_00165"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPW64543.1"
                     /translation="MIWKVREKIVAFDRTLLAGIVNVTHDSFSDGGKFLDPEKAAKHA
                     LRLVADGAEIIDLGAESTRPGSKSVAAAEEIKRLVPVIRAIRKKTDAILTIDTVKADV
                     AEKCLKEGADAVNDTSGLRAFGKKMAGVVKSYKAGLILMHSRGTPETMREMAEYRDLT
                     GEILKEIAGGVELALKCGVERERIMIDPGLGFAKNTEQNLAILRDPKPFLDTGYPVMV
                     GPSRKSFIGKVTGREPPDRDFGTAACVAACVAQGVQVVRVHEVKGMRDVILTTEAILK
                     A"
     gene            complement(2374..4299)
                     /gene="ftsH"
                     /locus_tag="PLY30_00170"
     CDS             complement(2374..4299)
                     /gene="ftsH"
                     /locus_tag="PLY30_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPW64544.1"
                     /translation="MPNGISNFKKQKKNGKLSKKKPEPEKNRVLWLFLVPLSILILLQ
                     VFVFPGLDTKEMSYSEFYRLLMANPENGQIFSCELVEDVVHGKLITGSYFQVHIPMND
                     LEIIPLMRKNIPNFSVSPPQTFWRNLVYSVMPVLLLIGFFWFFVYRGMQQGGSRVFSF
                     GKSRAKLAGKEKSPVTFKDVAGVDEAKEELQEIIEFLKDPHRFTKLGGKIPKGVLLMG
                     PPGTGKTLLAKAVAGEANVPFFSISGSDFVEMFVGVGAARVRDLFEQAKRAAKTGGKG
                     AIIFVDEIDAVGRQRFAGIGGGHDEREQTLNALLVEMDGFDTHAGVILIASTNRPDVL
                     DPALLRPGRFDRQVVVPLPDLIGREAILKVHAAKVKLAPGVDLKTIAKQTTYFSGADL
                     ANLVNEGALLAARMNKDAIGMTELQESIERVMAGPQRKSKKISDKERKIVAYHESGHA
                     LVALLTPGADPVHKVTIIPRGMAGGYTVTLPEEDRTLQSLSFFLAELPVILGGRVAEQ
                     VIFGDITTGAHNDLQKATQIARQMVCRFGMSEKLGPMIFGKENGMVFLGRDLMEERNY
                     SEETARLIDSEVKRIIEEAYAKAQKLLTENRDKLELLAKMLLEKETLDADEIRHIVGI
                     PLPSSGTQAPEGGTLAT"
     gene            complement(4447..5478)
                     /gene="tilS"
                     /locus_tag="PLY30_00175"
     CDS             complement(4447..5478)
                     /gene="tilS"
                     /locus_tag="PLY30_00175"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPW64545.1"
                     /translation="MRDLRSDLKARLEDAAFFRKNDRLLIACSGGPDSVALVHLFALI
                     APGNRWKLGILHYDHRLRSASKADAMFVRRLARGLGLAFYGGAGDVKRAALRTKTSLE
                     EAARKMRYDFFLRTAKRLKVKKVVLAHTLDDQAETVLMRILQGTGLRGLRGIREVNRM
                     EGVLFIRPLLAYAKSDLLDFLRTAKIGFRRDESNQSRRFLRNRIRLKLLPALRRDFNP
                     RIVESLARIPVIASREIALLDELEAGAWKKTFKKHERHKVELRRGDFLGLPVPIQFRV
                     IERALKKIDPRSGLSYEAWERIQKGLSRPRNRWSLPKHIDLTLTPKTMVLYKRNIPGK
                     QDHGEGNEV"
     gene            complement(5505..5577)
                     /locus_tag="PLY30_00180"
     tRNA            complement(5505..5577)
                     /locus_tag="PLY30_00180"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5542..5544),aa:Met,seq:cat)
     gene            5736..>6657
                     /locus_tag="PLY30_00185"
     CDS             5736..>6657
                     /locus_tag="PLY30_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPW64546.1"
                     /translation="MASKIHPKYASRTRPPVEPRKFDAVLFDIDNVLVDTRRSYLEAI
                     RYTVEIFLTHGDVPFFSKSSSGKMPELLSTHDVEKFKMLGGFNDDWDCCYGILVYLLS
                     LPVTNRTIEALKKASNFDKLVKSVKERPLKVSGITAIYGRPSTLVIEKVARIFQEVYL
                     GKDIFPRLTHNKMRYWKKRGLIDREKPIFKSPLLQKIKDAGMKLGICTGRSSYEAAYA
                     LKAFGMDKLFDAITTMDDVKQEERLKRESLRKPHPFSLLKTTEKLGKKLHYLYVGDLP
                     DDVLAANRAKKSVSICSAAFPMMAADPFRAQ"
BASE COUNT         1400 a         2033 c         1721 g         1503 t
ORIGIN      
        1 cggaccacaa aatgcgtcaa cagcttcatg gactgtcccg gatcagggag cgcggagaac
       61 gtattcaccg aaatcctgac ctcgtagccg atgccattca catccagcac cgccgcggcc
      121 ggcgtcttct cggttatttt acctgcaagg tattgataca tttcacttgc ctcgccgaat
      181 gggcatggca taaggctatc gccaacgcat cggaagcgtc aagcgcgggc gcttctttca
      241 aattcaaaag tcgtttgacc atgaactgca cctgctcctt cgatccgcga ccgttacccg
      301 tcacggagcg tttcacggct gtcggggcgt attctatcac ttcgatccct tccttagaag
      361 ccgcgagcat cgcgcaggcg cgcgcttcgc cgatcctttc aacagcgcga acgtccttcg
      421 cgaaaaaaag cgactcgatc gccaatgcgt cgggcttgta gctctggata ttcttctgga
      481 gggattcgaa catatggaga agacgtttcg cgatcgggtc cctggagttc gcgcggatcg
      541 tgccgcagat cacaaccttc acgtcccggc cggaggtctc gaggacccca aaacccatcc
      601 ggtgcgtacc cgggtcaaca cccatcacgc gcatggatca aacctcctga ccccatccaa
      661 cctacgaggt tggatacggg ttaacccgtg atctccttca ttacttcatc cggaatatcc
      721 gcgttcgtgt aaacgttctg gacgtcatcg tgttcttcga gggcctcgat cagtttcacg
      781 accgtgcgcc cggccgccac atccaccgtt gtcatattct tcgcgttcat ggtcagattc
      841 gcgctttcga tcttgatccc cgacttctcg atcgcatccc ggacagtcca aagatcggca
      901 ggcgccgtcg tcacctggaa cgtctctccg acggcggcca tgtcctcagc ccccgcgttc
      961 agcgccgttt ccatgagctt gtcttcggtc gtcgtcgagg tcgacacgac gatcaacccc
     1021 ttcttctcga acatccatgc cacgccgccg gcactgccca tgttcccgcc gttcttcccg
     1081 aaaatactgc ggatctcgga agcgcttcga ttcttgttat ccgtcaggca cgccacaagg
     1141 aacgcagcgc cacccggacc gaacccttcg taagtcactt cttcataagt aacgccttcg
     1201 agttcgccgg ttcccttctt gatcgcgcgc tcgatgttgt ccgccggcat gttcgcgtcc
     1261 ttgccggcct ggatcgccgt cctgagacgc gggttcattg aaggatcgcc gccgcccgtt
     1321 ttcgcggcaa cagtgatctc gcggatgatc ttcgtaaagg ccgcgccgcg tttcgcgtcc
     1381 gctgcggctt tcttgtgctt gatgctcgcc cacttattat gaccggacat gcctcacctc
     1441 gttagtgttc gtgctgtact ttttgtgttt aaaaatacga atttctgaat ccggatgaca
     1501 accgctcgac cgttcccggc attcgggcct catcatccgg gcctcaggcc ttcaggatcg
     1561 cttcggtcgt caagatcacg tcccgcatcc ctttcacctc atggacgcgc accacctgga
     1621 ccccctgcgc cacgcaggcc gccacgcagg ccgccgtccc gaaatccctg tccggcggct
     1681 cccggccggt cactttcccg atgaatgact ttctcgacgg ccccaccatc acgggatatc
     1741 ccgtatccaa aaatggcttc ggatcccgga gaatcgcgag gttctgttcc gtgttcttcg
     1801 cgaaacccag ccccgggtcg atcatgatcc gttcgcgttc cacaccgcac ttgagcgcca
     1861 attccacgcc ccccgcgatc tctttcagaa tctcgcccgt gagatcccgg tattcggcca
     1921 tttcccgcat cgtctccggc gtcccgcggc tgtgcatcag gatgaggccg gccttgtagc
     1981 ttttcacgac gccggccatc ttcttgccaa aggcccggag gccgctcgta tcgttgaccg
     2041 cgtccgcgcc ttccttgagg cacttttccg caacatcggc ttttaccgta tcgattgtaa
     2101 ggattgcgtc cgtttttttc cggattgccc ggataacagg cacaaggcgc ttgatttctt
     2161 cagcggccgc cacggatttc gatcccggtc gcgtggattc cgcgccgaga tcgattattt
     2221 ccgctccatc cgcaacaagc cggagcgcgt gtttcgccgc tttctccgga tcgaggaatt
     2281 tgccgccgtc cgagaacgaa tcgtgtgtca cgttcacgat ccccgcgaga agcgtccggt
     2341 caaatgcaac gatcttttcg cggactttcc agatcatgtt gcgagggtcc cgccctcggg
     2401 agcctgggtg ccggatgacg gcaacggaat cccgactatg tgccgtatct cgtcggcgtc
     2461 aagcgtttcc ttttcaagaa gcatcttggc aaggagttcc agcttatcgc ggttctccgt
     2521 caggagtttt tgggcctttg cgtaggcttc ttcgataatc cgtttgactt cgctgtcgat
     2581 gagcctcgcc gtctcttccg aataattccg ttcttccatg agatcgcgac cgaggaagac
     2641 cattccgttc tctttgccga agatcatggg gccgagcttc tcgctcatcc cgaaacggca
     2701 caccatctgg cgggcgatct gcgtcgcctt ctggagatcg ttgtgggctc ccgtcgtaat
     2761 atcgccgaaa ataacctgtt ccgcaacgcg accgcccagg atcaccggca gctccgcgag
     2821 aaagaaggaa aggctctgga gcgtccggtc ctcttccggc aacgtcacgg tgtaaccgcc
     2881 ggccatacca cgcggaataa tcgtgacctt gtgcacggga tcggcgcccg gcgtgagaag
     2941 cgccacaagc gcgtgccccg actcgtgata ggcgacaatc ttgcgctcct tgtccgaaat
     3001 cttcttgctc ttgcgctggg gacccgccat cacgcgctcg atggattcct gaagctccgt
     3061 catgccgatc gcgtccttgt tcatgcgcgc ggcaagcagc gcgccttcat tcacaaggtt
     3121 cgcaaggtcc gcgcccgaaa aataagtagt ctgtttggcg atggtcttga ggtccacacc
     3181 cggagcgagc tttaccttgg ccgcatggac cttgaggatc gcctcacggc cgatgagatc
     3241 cggaagcggc accacgacct ggcggtcgaa gcgacccggc cgcagaagcg ccggatcaag
     3301 aacgtccggc cggttcgtcg aagcgatcag gataacgccc gcgtgcgtgt cgaacccgtc
     3361 catttcgacc agaagcgcgt tcagcgtctg ttcgcgctcg tcatgcccac cgccgatccc
     3421 ggcaaatcgc tggcggccaa ccgcgtcgat ctcatcgaca aaaataatgg cgcccttgcc
     3481 gccggtcttc gcagcgcgtt tggcctgttc aaaaaggtca cggacacgcg cggcgccgac
     3541 accgacaaac atctcgacaa aatccgatcc gctgatcgaa aagaaaggca cgttggcctc
     3601 gcccgcgacc gctttcgcga gaagggtttt ccccgtaccc ggaggtccca tcaggaggac
     3661 ccctttcgga atcttgccgc cgagcttcgt gaaacggtgc ggatctttca ggaactcgat
     3721 gatctcctgc agttcttctt tggcctcgtc cacgcctgcc acatccttga aggtcacggg
     3781 gctcttttcc ttgccggcga gcttcgcgcg cgattttccg aaggagaaca cgcggctccc
     3841 cccctgctgc ataccgcggt aaacgaagaa ccagaagaaa ccgatcagaa ggagaacggg
     3901 catcacggaa taaacgaggt tcctccagaa cgtctgcggc gggctcaccg aaaaattcgg
     3961 gatattcttc cgcatcagcg ggatgatttc aagatcgttc atcgggatgt ggacctggaa
     4021 gtaggaccct gtgatgagtt tgccgtgcac aacgtcttcg accagttcgc aggagaaaat
     4081 ctgaccgttc tccgggttcg ccatcaggag acgatagaac tcggagtaac tcatttcctt
     4141 ggtatcgaga ccggggaaca cgaagacctg caacaggatc agaatggaga gagggacgag
     4201 gaacagccac agaacccggt tcttctcggg ctccggtttt tttttggaca atttgccgtt
     4261 ctttttttgc tttttgaaat tactgattcc gttaggcata cccattgcat cttccgatta
     4321 ttttattgat ggccccgcaa gctgacaaat cccgaaggga cttccgtttc tcagggcgta
     4381 ttcgagcgga cccatggaca ttttcaagat tgcgagcgcg tagtctagca cggccctttc
     4441 gagatatcaa acctcattac cctcaccatg atcttgtttc cccgggatat ttctcttata
     4501 cagcaccatc gttttggggg tcagcgtgag atcgatatgt ttcgggagcg accagcggtt
     4561 cctgggccgc gaaagacctt tttggatacg ctcccacgct tcataggaaa gcccgctccg
     4621 cggatcgatc tttttgagcg cccgttcgat aacgcggaac tgaatcggca cgggaagacc
     4681 aagaaaatcc ccgcgacgaa gctcgacctt atgccgttca tgctttttga aggtcttctt
     4741 ccatgctccg gcttccagct catcgaggag agcgatctcc cgcgacgcga tcaccgggat
     4801 tcttgcgaga gattccacaa tgcgcggatt gaaatcgcgg cgaagcgccg gcaacagttt
     4861 caggcgtatc cggttccgga gaaaccggcg cgattggttg ctttcatccc ggcggaatcc
     4921 gattttcgcc gtgcgaagaa agtcgaggag atcgcttttt gcataagcca gaagcggccg
     4981 gatgaataac accccctcca tccggttcac ttcgcggatt ccccggagac cgcgcaggcc
     5041 ggtcccctgc agaatacgca tcaacaccgt ttcagcctgg tcgtccagcg tgtgcgcaag
     5101 cacgactttt ttcaccttga ggcgctttgc ggtcctgaga aaaaaatcat agcgcatctt
     5161 ccgcgcggcc tcttcaagcg atgtcttcgt gcgcagcgcc gcgcgtttca cgtcccctgc
     5221 cccgccgtag aatgccaggc cgagcccccg ggcaagacgg cggacaaaca ttgcgtcagc
     5281 cttcgacgcg gaccgcaaac ggtgatcgta atgaaggata ccgagcttcc agcggtttcc
     5341 gggggcgatg agcgcaaaca aatggacaag cgcgacggaa tcgggacctc cggaacaggc
     5401 gataaggagc cgatcatttt tacggaaaaa agcagcatct tccaggcggg ccttgaggtc
     5461 ggaacggaga tcgcgcatga cgctccattt gttaaaatga aaaatgccgg agggcgggat
     5521 cgaaccgccg acacgaggct tatgagtcct ctgctctacc aactgagcta ccccggcaag
     5581 acagatcact gacaagggaa ataaaaccgc cgcatccttc tcaagcggtg gcgggtatac
     5641 tagcacaggc gccttcattc gccaagagcg gcccgtttca aaaaattaat gagaagtttc
     5701 tttacttacc gaaaagttgg gcgtaaactt ggttcatggc ttccaagatt catcccaaat
     5761 acgcatctcg tactcgccct ccggttgagc cgcgcaaatt cgatgctgtt cttttcgata
     5821 tcgacaatgt ccttgttgac acgcgccgct cttacctgga agctattcgg tataccgtgg
     5881 agatcttcct cacccacggc gacgtccctt ttttcagtaa atcttccagc ggtaaaatgc
     5941 ccgagctttt gtcgactcat gacgtggaaa aattcaagat gctcggcgga tttaacgatg
     6001 actgggattg ctgttacgga atactggtct atctcctgtc acttccagtt accaaccgga
     6061 cgattgaggc tttaaaaaaa gcttccaatt tcgacaaact cgtaaaatca gtcaaggaac
     6121 gacctctgaa ggtttccggc attaccgcga tttacggccg gccaagcacc ctcgtgattg
     6181 aaaaagtggc aagaatcttc caggaagtct acctgggcaa ggacattttc ccccgcttga
     6241 cccataacaa gatgagatac tggaaaaaac gcggactgat cgaccgggaa aaacctattt
     6301 tcaaaagccc cctcctccag aaaatcaagg atgctggaat gaaactcgga atttgcacgg
     6361 gccgatccag ctacgaagcg gcctacgcgt tgaaagcctt cgggatggac aaactttttg
     6421 atgccataac gaccatggat gacgtcaaac aggaagagcg cctcaaaagg gaatcgttaa
     6481 gaaaacctca tccgttttcg ctcctcaaga ccacggagaa actggggaaa aaacttcact
     6541 acctttatgt cggcgacctg cctgacgatg tcttggccgc aaatcgcgcg aaaaaatccg
     6601 tttcgatttg ttccgccgcc ttccccatga tggcggccga tccgtttcgc gcccaaa
//
LOCUS       DAOOBX010000006         6569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_13816_length_6569_cov_2.932607, whole genome shotgun sequence.
ACCESSION   DAOOBX010000006 DAOOBX010000000
VERSION     DAOOBX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13816_length_6569_cov_2.932607"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLY30_00190"
     CDS             complement(<1..485)
                     /locus_tag="PLY30_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64547.1"
                     /translation="MIWQLLPFISAPMSFQMAFDSVLFGKHLGNSRRETVLRFYLSSG
                     PWITAGFSFRSGDDLERSPLVKNHPAVPVARRITGGGCVLHGGDLVFTLIAPAAGRLE
                     TVKESYRDIHEAVREAFAGLGVDPRFYEETEDLPKGNDCFSYPVARDLAWKGRKIAGG
                     A"
     gene            complement(523..1947)
                     /gene="gcvPB"
                     /locus_tag="PLY30_00195"
     CDS             complement(523..1947)
                     /gene="gcvPB"
                     /locus_tag="PLY30_00195"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPW64548.1"
                     /translation="MPKERLAKASVPAGLARRDPLLFPELAELDAIRHFINLSRKNFS
                     VDTHFYPLGSCTMKYNPKINEWAARREKFAALHPLQKPETVQGMMRMLAELEDGLREI
                     TGMDRFTLQPAAGAHGELTGLMLVRAYHASRGDSKRMKVLVPDSSHGTNPATAALVGY
                     RVIEVKSNARGRVDLESLKAVLDDHVACLMLTNPNTLGVFEENILEIARLVHEKGGLL
                     YYDGANLNPLLGIARPGDMGFDIVHVNLHKTFSTPHGGGGPGAGPVGVKKHLVPFMPY
                     PLVEKKGDGVQWAPKPQRSIGRVKSFHGNIGVLLRAYVYLKMIGRDGLLRVSEGAILN
                     ANYLREKLRTVFPVAVDEPCLHEFVLSLKQAAAKGVHAGDVGKRLLDFGFHAPTVHFP
                     LVVSAALMIEPTETESRETMDLFIEAMKRIAFEIETEPAKVKNAPYTLPVRRPNDVLA
                     ARKPILTYRDLLREEAVAAEARVG"
     assembly_gap    1959..1968
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2014..3351)
                     /gene="gcvPA"
                     /locus_tag="PLY30_00200"
     CDS             complement(2014..3351)
                     /gene="gcvPA"
                     /locus_tag="PLY30_00200"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPW64549.1"
                     /translation="MPYIPLTGPEKRGLLREIGVSSLDDLIRAIPSALRGPEMSLPEG
                     MSEPEVQHLVRDLGALNTSGLDFLSFLGAGSYDHFIPSAVQEVISRQEFSTAYTPYQP
                     EASQGTLQAIFEYQSLMAELTGLAASNASHYDGATSLAEAALVALRHTERPKILIARS
                     VHPHHREVIRTYLAESTCRVEEFGFTAGGGFDRQSLAAKLDADTAGVIFQTPNFFGIV
                     EDLAGIADQVHANKSLLILSGHPASFALFRSPGEWGADIAAGEGQPLGMPVSFGGPYL
                     GYFVTTQALLRRMPGRIAGATEDADGRRAFCLTLQAREQHIRRERAGSNICSNQALCA
                     LAACAYMTFLGRQGMREVAELNLDRATYLREKIAGIKGFEVAVSQPIFNEFVVKSPVP
                     FAQIEKKLAAERIFPGVALETYYPELRNRFLVCATETKTREDIDRFAEALAKC"
     gene            complement(3365..3757)
                     /gene="gcvH"
                     /locus_tag="PLY30_00205"
     CDS             complement(3365..3757)
                     /gene="gcvH"
                     /locus_tag="PLY30_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPW64550.1"
                     /translation="MNFPENLRYTRTHEWIALTGNKGKVGVTEYAQKEISDVVFVELA
                     RKGHAVAQGKAASVVESVKAAFDIYAPLAGVISDFNQALESNPGLVNQDPYGAGWLFE
                     LEVTDPSALNTLMTAAQYAEFIQSGAGH"
     gene            complement(3809..4909)
                     /gene="gcvT"
                     /locus_tag="PLY30_00210"
     CDS             complement(3809..4909)
                     /gene="gcvT"
                     /locus_tag="PLY30_00210"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPW64551.1"
                     /translation="MTLETVSKKLPLYAEHVALGARMGEFGGWEVPLYYSSVIEEHKA
                     VRAAAGIFDISHMGEILVEGPRALEFLERNLPRRIAPVKLQQAIYTPLLNDRGGMVDD
                     IIVYRVREDRFLIIVNAGNIAKDFEWFCARAPKGAVVTDLSPQYGLFAVQGPEAAAIV
                     SAAFGVFFTQLKRFQYRDFEGGIIARTGYTGEDGFEIMVPLERLKPVWDVLLEVGGRK
                     GLKPVGFGARDTLRLEAAMPLYGHELSDEITPLEAGLEWAIDLTKDEFPGLEVLKVQK
                     AAGIPKKRVGFEMTGRGIPRHECEVRQNGRKVGWVTSGSFIPAHGSFAGRNIGMAFVE
                     TAQAETGTEIDVMIRGAAAGARVVSLPFYKRK"
     gene            complement(5019..6272)
                     /locus_tag="PLY30_00215"
     CDS             complement(5019..6272)
                     /locus_tag="PLY30_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64552.1"
                     /translation="MTQPAPDLKQKCAVIYIGTRKLVAVLADLREVSARVLHKEEVIF
                     PDGFEKGFVCNLQNAAATVEKLLGALLPAEAWEHVPVYVVLGNKGFRMRRHSSCEYYT
                     PEKRTISPNEVQSVVQQTRSVAMLPLTEAILQAVPESFLVNDMPAVRNPVGLEAERLG
                     VTLQLYTMEFQSFRNIMKVFEAIDVDVQGFFPKTLTIPEAVLTDAEKQEGVLVIDVAD
                     DATQFMLWKNGYLADIRSFDHGGHYLTECIARDYHIEERDADKVKSKFATLDEGDRLS
                     EELVPLVTRNEKVQQSVRRRELVDHFFLHAKTWMNGILEDSGKFIQDEKVRHPHIIFT
                     GGSVMIDGFLEWLQKEFGVEARLGVTRQVEAAQEIMRDPSLTPVTGMLRWIKTAKREN
                     EALFTSRSLLTRLAAAIRRWFSDYF"
     gene            complement(6269..>6569)
                     /locus_tag="PLY30_00220"
     CDS             complement(6269..>6569)
                     /locus_tag="PLY30_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64553.1"
                     /translation="RIGSRILNKGFFEALKFLKAFERHELSRKEKITKISLDPYGNVA
                     VRLGEGPDFLMGRRPTEKFAQLSKAMYLFKTEPRENFEYVDLQYDRVVGNPRKQT"
BASE COUNT         1404 a         2020 c         1844 g         1291 t
ORIGIN      
        1 tgggcgcccc ccgcgatctt ccgccccttc cacgcgagat cacgcgccac gggatacgag
       61 aaacaatcgt tccctttcgg aaggtcctcg gtctcctcat agaatcgcgg atcaaccccc
      121 aagcccgcga acgcctccct gacggcttca tggatatcgc ggtagctctc cttgaccgtt
      181 tcgagacgtc ccgccgccgg cgcgataagc gtaaaaacaa ggtccccgcc gtgaaggaca
      241 caacccccgc ccgtgatccg ccgcgcgacc gggaccgcag gatggttctt cacaagcggg
      301 gaacgctcaa gatcgtcccc ggagcgaaat gaaaaaccgg cggtaatcca gggcccggag
      361 gaaagataga acctgaggac ggtttcgcgg cgggaattcc cgaggtgctt tccgaacagg
      421 acactatcga acgccatctg aaaggacatc ggcgcgctga tgaatggaag aagttgccag
      481 atcatgccgt gaaggaatcg accgtgccgg agcacgtaac gcttagccca cccgggcttc
      541 tgctgcgacg gcttcctcgc ggagcagatc acggtaggtc aggatgggtt tacgcgcggc
      601 aagaacgtcg ttcggccgtc tcaccggaag cgtgtacggg gcgttcttga ccttggccgg
      661 ctccgtctcg atctcgaaag cgatccgctt catggcctcg ataaagaggt ccatcgtttc
      721 ccggctttcg gtctcggtcg gctcgatcat gagggccgcg gagaccacca gcgggaaatg
      781 gaccgtcggg gcatgaaaac cgaaatcaag aagacgcttg ccgacatcgc cggcatgaac
      841 gcctttcgcg gcggcctgct tgagcgacag cacgaactcg tgaaggcagg gctcatccac
      901 cgcgaccggg aacaccgtcc tgagtttttc gcggagataa ttcgcgttca ggatggcgcc
      961 ctcgctcacg cggagcaggc cgtcgcggcc gatcatcttc agatacacat acgcgcggag
     1021 cagcacgccg atattcccgt gaaaggattt cacgcgcccg atgctccgct gcggcttcgg
     1081 cgcccactgg acgccgtccc ctttcttctc gacaagcgga tacggcatga acggaacaag
     1141 atgctttttc actccgaccg gtccggcccc cggaccgccg ccgccgtgag gcgtggaaaa
     1201 agtcttatgg agattcacgt gcacgatatc gaaccccatg tcgcccggcc gcgcgatgcc
     1261 aagcagcggg ttcaggttcg ctccgtcata atagagcagg ccgccttttt catggacgag
     1321 ccgggcgatc tcaaggatgt tttcctcgaa cacacccagt gtattcgggt tcgtgagcat
     1381 cagacatgcc acatggtcat cgagcaccgc cttcaggctt tcgaggtcca cgcggccgcg
     1441 ggcgttcgat ttcacttcga tcacacgata accgaccaac gcggcagtcg ctggattcgt
     1501 cccgtggctt gaatccggca ccagcacttt catgcgcttt gagtccccgc gcgacgcgtg
     1561 ataggcccgg accagcatca ggccggtcaa ctcaccgtgc gcgccggcgg cgggctggag
     1621 cgtgaagcga tccatgccgg tgatctccct gagcccgtct tcgagttcgg caagcattcg
     1681 catcattccc tgcaccgtct ccggcttctg caacgggtgg agcgcggcga acttttcccg
     1741 gcgcgcggcc cattcgttga tcttcgggtt gtacttcatc gtgcagctgc caagcggata
     1801 aaaatgggta tcgaccgaaa aatttttccg ggacagattg ataaaatggc gtatggcgtc
     1861 aagctcggca agctccggga aaagaagcgg atcccgccgc gcgagaccgg cgggcacgga
     1921 ggccttcgcg agcctttcct tcggcatcgt gacacagcnn nnnnnnnngt cccgggttgc
     1981 tcttttcaaa actgagacgg tcatccctgg agatcagcat ttggccaacg cctccgcgaa
     2041 ccggtcaata tcctcgcgcg tcttcgtctc ggtcgcgcac acgagaaagc ggttcctgag
     2101 ctcggggtaa taggtttcaa gcgcgactcc cgggaagatc cgttccgcag ccagtttctt
     2161 ttcgatttgc gcgaacggca cgggactttt cacgacgaat tcattgaaga tcggctgcga
     2221 tacggcgacc tcgaagccct tgattccggc gatcttttct cgcaggtaag tggcccggtc
     2281 caggttgagt tccgcgacct cccgcatgcc ctgcctgcct agaaacgtca tgtaggcgca
     2341 ggccgccagc gcgcaaaggg cctggttgga acaaatattg gatcccgccc gctcacggcg
     2401 aatatgctgt tctcgggcct ggagcgtgag gcagaacgcc cgtcttccgt cggcgtcttc
     2461 cgtggctccc gcaatacggc ccggcatcct gcgaagaagc gcctgggtcg tgacaaagta
     2521 tccgagatag ggaccgccga acgacaccgg catcccgaga ggctgtcctt cccccgccgc
     2581 gatgtccgca ccccattccc cgggggaacg aaagagggca aaagaggcag gatgtccgga
     2641 aaggatcaga agggatttgt tggcgtggac ctgatccgcg atccccgcaa ggtcctcgac
     2701 gatcccgaag aaattcgggg tctgaaagat cacgcccgcc gtatcggcat ccagcttcgc
     2761 ggcgagactc tggcggtcaa aaccaccgcc ggccgtgaac ccgaactcct cgacgcgaca
     2821 ggtgctttcc gcaaggtagg tgcggatgac ttcccggtga tgcggatgaa cggaccgggc
     2881 aatgaggatc ttgggacgct ccgtgtgacg caacgcgaca agcgccgctt cggcaaggct
     2941 cgtcgcgccg tcataatgag aagcgttcga cgccgcaagg cccgtcagtt cagccatgag
     3001 gctctgatac tcaaagatcg cctggagggt tccctgcgaa gcctcgggct gatacggcgt
     3061 gtaagcggtg ctgaactcct ggcgcgagat gacctcctgg accgcggaag ggatgaagtg
     3121 gtcataactt cccgcaccca gaaagctgag aaaatcaaga ccggaggtat tgagagctcc
     3181 gagatcccgt acgagatgct gaacctcagg ttcgctcata ccttccggaa gcgacatctc
     3241 cggaccgcgg agcgccgacg ggatagcgcg gatcagatca tcaagtgaag ataccccgat
     3301 ttcccgaaga agaccccgtt tctcgggccc ggtcagtgga atgtacggca tcgcggtccg
     3361 ggtatcagtg gccggcgccg gactggataa actcggcgta ctgcgcggct gtcatgaggg
     3421 tgttcagagc ggaggggtcc gttacctcca gctcaaagag ccatcccgcc ccatacgggt
     3481 cctggttcac gagacccgga ttggattcaa gcgcctggtt gaaatccgag atgacccccg
     3541 ccagaggagc ataaatgtca aacgccgcct tgaccgattc gacgacagaa gccgccttgc
     3601 cctgcgcgac cgcgtggcct ttcctggcaa gctcaacgaa caccacatcc gaaatctcct
     3661 tctgggcgta ctcggtcacg ccaaccttcc ccttgtttcc ggtcaacgcg atccattcgt
     3721 gggttctggt gtaacgcagg ttctcgggaa aattcatgca atcctccggt aaaatttgga
     3781 agttcaaaca aaatgacgcg gcattctact attttcgctt gtaaaaaggt agggaaacca
     3841 cgcgcgcccc ggccgcggcg ccgcggatca tgacatcgat ctcagttccc gtctcggctt
     3901 gcgccgtttc aacaaaggcc atgccgatat tccggcccgc gaagctcccg tgcgcaggga
     3961 taaaactccc gctcgtgacc catccgacct tccgtccgtt ctgccgaacc tcacattcat
     4021 ggcgcggaat cccccggccc gtcatctcga atccgacgcg ttttttcgga ataccggcgg
     4081 ccttctgcac cttcaacacc tccagccccg ggaattcatc cttggtcaga tcgatggccc
     4141 attcaaggcc ggcttcaaga ggagtgatct catcacttaa ttcatggcca taaagaggca
     4201 tcgcagcctc aaggcgcagg gtatcgcgcg cgccaaagcc aacgggtttg agcccctttc
     4261 ttcctccgac ctccagaagt acatcccaga cgggcttgag acgctcgaga ggcaccatga
     4321 tctcgaaacc atcctcgccc gtgtagcccg ttcgggcgat gatcccgccc tcgaaatcgc
     4381 ggtactggaa tcgcttcagc tgcgtaaaga aaacgccaaa cgctgcggag acgatcgcgg
     4441 ccgcctcggg accctggacc gcgaagagac cgtactgggg cgaaaggtcc gtcacgaccg
     4501 cgcctttcgg agcgcgggcg cagaaccatt cgaaatcctt cgcgatattt ccggcattga
     4561 caatgatgag gaaccggtcc tccctgacgc gatagacaat aatgtcatcc accatccctc
     4621 cgcgatcatt caggaggggc gtgtaaatgg cctgctggag cttgacggga gcaatgcgcc
     4681 gcggaagatt ccgctcgagg aattccaggg cccggggccc ttcgacgaga atctccccca
     4741 tatgggaaat atcgaaaata ccggccgcgg cacgaaccgc cttgtgctct tcgatgacgc
     4801 tcgagtaata gagaggcact tcccacccgc caaattcacc catgcgggca ccgagagcga
     4861 cgtgctcggc atacaaagga agttttttcg aaacggtctc cagggtcatc ggtttctctg
     4921 gggtttaaca aggataaaaa atatcgaaat gaacggcgcg gattatagcg aaaaaagcgc
     4981 cgaaacaaaa gatggatttt ccggacggag aggaaaaatc agaaataatc cgagaaccag
     5041 cgacggatcg ccgccgccaa acgggtcaga aggctgcggg aggtaaaaag cgcctcattt
     5101 tcccgcttcg cggtcttgat ccagcggagc atgcccgtaa cgggggtcag cgaaggatcc
     5161 cgcatgatct cctgcgccgc ctcgacctgc cgggtcactc ccaaccgggc ctcgaccccg
     5221 aactcttttt gcagccattc gaggaatccg tcgatcatga ccgaaccgcc cgtgaagatg
     5281 atgtgcgggt ggcggacctt ttcatcctga atgaatttcc cggaatcctc gagaatcccg
     5341 ttcatccagg ttttcgcgtg taaaaaaaag tgatcgacaa gctcccggcg acgcaccgac
     5401 tgctgaactt tctcattacg ggtgacaagc ggcacaagct cctcgctcag ccgatcccct
     5461 tcatccaggg tagcgaattt ggatttcacc ttatcggcgt ccctctcctc gatgtgataa
     5521 tccctcgcga tgcattcggt gaggtaatga ccgccatggt caaaactccg gatatcggcc
     5581 agataaccgt tcttccagag catgaactgc gtggcgtcat ccgcgacatc gatcacgaga
     5641 acaccctcct gcttttccgc gtcggtcagc accgcttccg ggatcgtcag tgttttcggg
     5701 aaaaacccct ggacatccac atcgatcgct tcaaacacct tcatgatatt ccggaagctc
     5761 tggaactcca tcgtgtacag ttgcagcgtg acaccgaggc gctccgcttc gagtccgacg
     5821 ggattacgaa cggccggcat atcattgaca aggaacgatt cgggtaccgc ctgaaggatc
     5881 gcttccgtca aaggcaacat ggcaacgcta cgcgtctgct ggacgaccga ttgcacctca
     5941 ttcgggctga tggtccgttt ctccggcgtg tagtactcgc agctcgaatg gcggcgcatc
     6001 ctgaaccctt tattgccgag caccacatag accggcacat gttcccaggc ctcggccggg
     6061 agaagcgctc caagaagttt ttcaaccgtt gcggcggcgt tctggagatt gcaaacaaaa
     6121 cccttttcaa acccgtccgg gaagatcacc tcttccttgt ggagcacgcg ggccgagacc
     6181 tcccgaaggt ccgcgagcac cgcgaccagt ttccgggtcc cgatatagat tacggcacat
     6241 ttttgtttga ggtccggagc cggctgtgtc atgtttgttt tctcgggtta ccgaccacac
     6301 ggtcgtactg gagatccacg tactcaaaat tctcccgcgg ctccgtcttg aacaggtaca
     6361 tcgccttcga gagctgggcg aacttttcag tcgggcggcg ccccatcaaa aagtccggcc
     6421 cctcgcccag acgaaccgcg acgttcccgt acggatcgag ggatatcttc gtgatttttt
     6481 ctttacggga aagctcgtgc cgctcaaacg cttttagaaa tttgagcgct tcgaagaatc
     6541 ccttgttcag tatgcgagaa ccgatcctg
//
LOCUS       DAOOBX010000007         6466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_14074_length_6466_cov_3.254874, whole genome shotgun sequence.
ACCESSION   DAOOBX010000007 DAOOBX010000000
VERSION     DAOOBX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14074_length_6466_cov_3.254874"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1082
                     /gene="gpmA"
                     /locus_tag="PLY30_00225"
     CDS             333..1082
                     /gene="gpmA"
                     /locus_tag="PLY30_00225"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPW64554.1"
                     /translation="MYKLVLIRHGESIWNKENRFTGWYDVDLSEKGLQEARDGGALLK
                     KEGYTFDVAFTSVLKRAIRTLWNVLDQMDLMWIPVIRDWRLNERHYGALQGLNKSETA
                     VKYGEAQVKIWRRSYDTPPPKLEPNDPRSPAFDPRYKNLKPNELPLTECLKDTVARVI
                     PFWNDTIAPAIKSGKKVLVAAHGNSLRAMVKFLDNIPESEIVELNIPTGMPLVYELDK
                     DLKPIRHYYLGDPEKIKAAMEAVANQGKAKK"
     gene            complement(1278..2405)
                     /gene="hemW"
                     /locus_tag="PLY30_00230"
     CDS             complement(1278..2405)
                     /gene="hemW"
                     /locus_tag="PLY30_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPW64555.1"
                     /translation="MKKGLYIHIPFCKSRCHYCHFISIADPGPDVRRRFFNCLFRELD
                     HARAEHGAIKFDTIYFGGGTPSLLTEDEMTEIVNRIRAAFGVRPGAEITCEWNPGDGD
                     EEKLRAFSALGANRISLGAQSFRDDILQRLGRRHTAHDTIVTLDRIRGAGITNVSLDL
                     MLRLPGQTVVDFRDSLCRAVGLGVSQVSLYDLEIHEETVFGRMQREQKLDLPAEQVHA
                     AMYEAAIRTLTGAGYEHYEISNFARPGFASRHNLIYWRNEEYLGLGPGSFSYLAGVRS
                     GFAHDLEGYFRKCETGEWTNDVEDVLSEEDKETESFTMRLRLCGGAAPSRFPVLYERI
                     KDRARELLDGGFLEWAGDSLRLTTRGKFLSEDVFGFLLRKE"
     gene            complement(2481..3158)
                     /locus_tag="PLY30_00235"
     CDS             complement(2481..3158)
                     /locus_tag="PLY30_00235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPW64556.1"
                     /translation="MLKILMTRFRQGCRTMAFPKRKPPVLPARYRGRPLVAGPEEEID
                     LGKITFVPDSSIRHSNDFRMATCRRQDLVLQGETYALARAVNRKIVKLFGRSLKLRQV
                     SAGGCNACEADTNVLNTPVFDLSRFGIRFVASPRHADGVLVTGPVTRNMKEALLKTYE
                     AVPDPKIVIAVGACAISGGIFSRSPEVSRGLDTIIPVDLYIPGCPPHPMTILDGFLRL
                     LGRLKKE"
     gene            complement(3190..4701)
                     /locus_tag="PLY30_00240"
     CDS             complement(3190..4701)
                     /locus_tag="PLY30_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HPW64557.1"
                     /translation="MELFNGVRSFWKDVPLLAEKEFRETVILRCREGARLANLFGIPE
                     EGGDIRLAAILAADENPELEVLSMRVSPGSPRYVALTPELPEAHLFEREIAEQWAVLP
                     EGHPWLKPVRYHVNYRGGPDLWDEIAKRTIPGVYPFYAVDGEEVHEVGVGPVHAGIIE
                     PGHFRFQCHGEEVMHLEIQLGYQHRGIEPMMCGRDLNRSVLLSESVAGDTSIGHSLAF
                     CEVIESLAGCAVPLKAQAIRAIALELERLTNHVGDLGALGMDAGFLPTSAYFGRMRGD
                     YLNLLLELTGNRFGKGLCRPGGVLFDMEASMAEDFRRKLRRYQKEFEEVSDLLFSRPS
                     VLARFEGIGTVSPDDAKKMGMVGPAARASQVERDARVDHSSGIWRFYHVPISLAVTGD
                     VYARALVRRQEVMRSLEFLQRILASLPQGAIFSAPGPLAPGALAVSMVEGWRGEIMHV
                     AFTDGRGGLRRYKIKDPSFHNWFALTLAMRGQEISDFPLCNKSFNLSYSGHDL"
     gene            complement(4710..6122)
                     /locus_tag="PLY30_00245"
     CDS             complement(4710..6122)
                     /locus_tag="PLY30_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPW64558.1"
                     /translation="MTLILLIAVPLIAALIVLLPAKNIFRVRLLCAVSLFHASVVASF
                     WWRRPGPELQGYFVLDPLGLLLLSILDLLFLATSFFMIGYFEREHRNNRTFTACILCL
                     LSMTTMVSVSYHLGLMWVAIETTTLFSAPLISFHRNWKSLEATWKYLLVCSVGIALAL
                     MGTFFLAIAAHETRTLLLPAILNNAPLLSTSWLKLSVIFLFVGYGCKMGLAPMHSWKP
                     EAYGQATGMVSVLMAGGLTQCSFLALMRVSQICHKTGLGDFYGSILIVAGVLSLVVPA
                     VFLLDEKSLKRSFAFSSVEHVGLLVLGLGMGGVGVFGALFHMINNALAKVVMFLTAGS
                     LAQKYGSSRAPEVRGVIHLYPVTGVFLLVALLAGTGMFPFATFHSELLIINASVISEH
                     YVLAGIIIVCLAVIFMGISRLVLDLVYGKPVEPAPAPRENLFMNITIAAAAALLVGLG
                     LWMPAPLSGLLHEAAALIGA"
     gene            complement(6122..>6466)
                     /locus_tag="PLY30_00250"
     CDS             complement(6122..>6466)
                     /locus_tag="PLY30_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64559.1"
                     /translation="LIAVAFWISLAMKLPGKLESSLVLPCSLATILLGFMTMVTRTRA
                     VTQVLGYMVIENGIFLFALSLFDTMPVPIEMGILLDIFVAVFVMAIVLNHINEEFERT
                     RSTGRRPRWGES"
BASE COUNT         1480 a         2008 c         1828 g         1150 t
ORIGIN      
        1 cgtcgctccc gcggattcat ctcctccgct tttcgcaggg cccggtaaaa tcaaatccgc
       61 ggacgctcac cgataaacac aaaacaagtg tccctgtttc tggccggcga aactccgctc
      121 tcttcgagga acagaaaggt tggaggtcat gtgtcagtcc cgcagaaaag aaaggctgaa
      181 acgcgggtcg tgcggttttt gcagacggat tttttatttc cctgccagaa ggatagtgtt
      241 acactatcgc ccttccatca taaaaagaac gggggcactc gttttttgcc catagcagga
      301 gcggccccgt attccccaag gaggatttgg taatgtacaa gctcgttctg attcgtcacg
      361 gcgaaagcat ctggaacaag gagaaccggt tcaccggctg gtatgacgtg gacctctccg
      421 aaaaaggtct tcaggaggcc agggacggcg gcgcccttct caaaaaagaa ggctacacct
      481 tcgacgtcgc cttcacctcc gttctcaaac gcgcgatccg caccctgtgg aacgtcctcg
      541 atcagatgga cctgatgtgg atccctgtca tccgggactg gcggctgaac gagcgtcatt
      601 acggggcgct gcagggtctc aacaaatccg aaacggccgt caagtacggc gaggcgcagg
      661 tcaagatctg gcgccgcagt tacgacacgc cgcccccaaa gcttgaaccg aacgatccac
      721 ggagcccggc gttcgacccg cgctacaaga acctcaagcc gaacgaactg ccgctcacgg
      781 aatgcctcaa ggacaccgtc gcgcgcgtga tccccttctg gaacgatacg atcgcgccgg
      841 ccatcaaatc cggcaagaag gtcctcgtcg cggcgcatgg caacagcctc cgcgcgatgg
      901 taaaattcct cgataatatc cccgaaagcg agatcgtgga gctcaacatc ccgaccggca
      961 tgccgctcgt ctacgagctc gacaaggacc tgaagccgat cagacattac tatctcggcg
     1021 atccggaaaa gatcaaggcg gcgatggaag ccgtcgcgaa ccaggggaag gcgaagaaat
     1081 aatcgccacg cgtgccgcgc cgcacgaaga agatccttct ctcgcggcat gtgctgtgcg
     1141 gctttttgtc cggtttgaac agaaaggaaa aataacgcgt ctcgggttca tggcgacccg
     1201 ccggctcttc atcttcggcg tgagttttcc gcttgccggc atcgccgccg cttgagcctg
     1261 ttcgccgaag aaccgtctta ttctttccga agcaggaacc cgaaaacgtc ttccgagaga
     1321 aatttccccc gcgtcgtgag tctcagcgaa tcaccggccc attccagaaa tcccccgtcg
     1381 agaagttccc gggccctgtc cttgatcctc tcgtagagaa ccggaaaacg ggatggggcc
     1441 gcacctccgc aaagcctcag ccgcatcgtg aaactttcgg tctccttgtc ttcctcgctc
     1501 aagacatctt cgacatcgtt cgtccactca ccggtctcgc acttcctgaa atagccttcg
     1561 aggtcatgcg cgaatccgga acggacaccg gcaagataag agaatgaacc gggccccagg
     1621 ccgagatatt cctcgttgcg ccagtagatg aggttgtgcc gcgaggcgaa acccggccgt
     1681 gcgaagttcg agatctcata atgttcgtac ccggcgccgg tgagcgtgcg gatcgcggct
     1741 tcatacatcg ccgcgtgtac ctgttcggcc ggtaaatcga gcttttgctc gcgttgcatg
     1801 cgcccgaaga ccgtctcctc gtggatttcg aggtcgtaaa gggaaacctg ggaaacgccg
     1861 agcccgaccg cgcggcagag cgaatcccgg aaatccacga ccgtttgtcc cgggaggcgc
     1921 aacatgagat ccaggctgac attggtgatc ccggcgccgc ggatccggtc gagcgtcacg
     1981 atcgtgtcat gggccgtatg ccgtcgtccg agcctttgga ggatatcatc ccggaatgac
     2041 tgggcaccga ggctgatccg gttcgcaccg agcgccgaaa atgccctcag tttttcttca
     2101 tcgccatcgc cggggttcca ttcgcaggtg atctccgccc ccggccggac cccgaacgcc
     2161 gcgcggatcc tgtttacaat ctccgtcatt tcgtcctcgg tcaaaagcga gggcgtgccg
     2221 cctccgaaat agatcgtatc aaatttaatc gcgccgtgtt ccgcgcgcgc gtggtcaagc
     2281 tccctgaaaa gacagttaaa gaatctccgc cgcacatccg gcccggggtc cgcgatcgag
     2341 ataaaatggc agtaatggca cctcgacttg cagaatggga tgtggatgta taaacctttt
     2401 ttcatgatgg attatagccc cttctccggc cgcaggggcg atatcttgcg ggaaggcgcc
     2461 tgtgggattg ccggatccgg ctattccttt ttcagccgcc cgagcaatct caagaatccg
     2521 tcgaggatgg tcatcggatg cggcgggcag ccgggaatat aaaggtccac cgggattatc
     2581 gtgtccaggc cccgggacac ttcgggactc cgggaaaaga tcccccccga gatcgcgcag
     2641 gcgcccacgg cgatcacgat tttggggtcc ggcaccgcct cataggtctt gaggagcgcc
     2701 tctttcatat tacgcgtcac ggggcccgtg acgaggactc cgtcggcatg acgcggcgag
     2761 gccacgaacc ggataccgaa gcggctgagg tcgaaaacgg gggtgttcag gacgttcgta
     2821 tccgcctcgc aggcattaca cccccccgct gaaacctgcc tcaatttgag agacctgccg
     2881 aacaacttca cgatctttcg attcaccgcg cgcgccaagg catacgtttc gccctgaagg
     2941 acgagatcct gccggcggca tgtcgccata cggaagtcgt tggaatgacg gatgctgctg
     3001 tccggaacaa aggttatctt tccgagatcg atctcctcct ccgggcctgc cacgagggga
     3061 cgaccgcggt agcgggcggg cagaaccggc ggttttcgtt tgggaaaagc catcgtccgg
     3121 cacccctgcc tgaaacgcgt catcaagatc ttcagcatac cgggacctcg cttttattcg
     3181 cggcgcggat catagatcgt gcccgctgta ggacaaatta aagctcttgt tgcacaatgg
     3241 aaaatccgaa atctcctgac cgcgcatggc aagagtcagc gcgaaccagt tatggaacga
     3301 ggggtccttg atcttgtaac ggcgcaggcc tccccggccg tcggtgaacg ccacatgcat
     3361 gatttcacca cgccatcctt cgaccatgga aacagccagc gcgcccggcg cgagaggccc
     3421 gggggcggaa aaaatcgcac cctggggcaa agaggcgaga atcctctgca ggaattccag
     3481 ggagcgcatc acctcctgcc gtcgcaccag cgcccgggcg taaacgtccc cggtgaccgc
     3541 gagcgaaatg ggaacatgat aaaaccgcca gatcccggaa gaatgatcga cgcgcgcatc
     3601 acgctccacc tgactcgcgc gcgcggcggg cccgaccatc cccatctttt tcgcgtcatc
     3661 gggggaaacg gttccaatgc cttcaaaccg cgcgaggacc gagggccggc taaaaagcag
     3721 atcgctcacc tcttcaaact ccttttgata acgtcgaagc ttccggcgaa aatcttcggc
     3781 catggaggcc tccatatcaa aaaggacccc gccggggcgg cataaccctt taccgaagcg
     3841 gttccccgtc aactcaagca gcagattcag ataatcaccc cgcatgcggc caaaatacgc
     3901 gctggtcgga agaaaccccg cgtccatccc gagcgcgccc aaatccccca cgtggtttgt
     3961 gagccgctcc agttcgagcg cgatcgcgcg gatggcctgc gccttgagcg gcaccgcgca
     4021 gcccgcaagg gattcgatga cctcgcagaa ggccagggaa tgtccgatgg aggtatcccc
     4081 cgcgacggat tcgctcaaaa gcacactccg gttcaggtcc cgcccgcaca tcatcggttc
     4141 gatcccgcgg tgctggtagc ccaactggat ctcaagatgc atcacctctt cgccgtgaca
     4201 ctggaaacgg aaatgccccg gttcgataat gccggcatgc acggggccca cacccacttc
     4261 atggacctct tcgccgtcca ccgcataaaa aggatagacc ccggggatcg tccgcttcgc
     4321 gatctcatcc cagaggtcgg ggccgccccg gtaattcaca tgatagcgga cgggtttgag
     4381 ccaggggtgt ccttccggga gaaccgccca ttgttccgcg atctcgcgct cgaaaagatg
     4441 cgcttcggga agctccggcg tcaacgcgac ataccgggga gatcccgggg agaccctcat
     4501 cgacaacacc tcaagctccg ggttctcgtc cgccgcgaga atcgcggcca aacgaatatc
     4561 ccctccctct tccgggatcc cgaacagatt ggcaagccgc gcgccctcac gacagcgaag
     4621 gatcactgtc tcgcgaaact ctttttccgc gagaagcggg acatccttcc agaaactacg
     4681 cacaccgtta aacagttcca tcgatcctct caggccccga tcagggccgc cgcttcatgc
     4741 aaaagaccgc tcaagggggc cggcatccag agtcccagcc cgacaagcag cgcggcggcg
     4801 gccgctatgg tgatattcat gaataaattc tcgcgcgggg ccggggccgg ttctaccggt
     4861 ttgccgtaga cgagatccag cacgagcctc gaaatcccca tgaaaatgac ggccagacac
     4921 acaatgatga tcccggccag gacgtaatgc tccgagataa cggaggcgtt gataatcaaa
     4981 agttcgctgt gaaaggtcgc aaacgggaac atcccggtcc ccgccagaag cgccacgagc
     5041 agaaaaacgc ctgtcacggg atagagatga atgacccccc ggacctccgg ggcgcgggac
     5101 gatccgtatt tctgggcaag ggaccccgcc gtgaggaaca tcaccacctt ggcaagggca
     5161 ttattgatca tgtggaacaa ggcgccgaag accccgactc cgcccatgcc gagcccgagg
     5221 accagcaatc ccacatgctc gaccgaggag aacgcgaagg accgcttgag gctcttctca
     5281 tccaggagaa ataccgcggg aaccacgagc gacagaacac cggccacgat caagatcgat
     5341 ccgtagaaat cgccgagccc tgttttgtga cagatctggc tcacgcgcat caacgcgagg
     5401 aaagagcact gggtcaatcc ccccgccatc aggaccgaca ccatacccgt cgcctggcca
     5461 taggcctccg gcttccagct gtgcattggg gcaagcccca tcttgcaacc gtatcccacg
     5521 aacaggaaaa taacgctcaa tttcagccag ctggttgaaa gcaggggcgc gttgttcagg
     5581 atcgcgggca ggagaagcgt acgcgtctcg tgggccgcga tcgcgaggaa gaacgttccc
     5641 atgagcgcga gcgcgatccc gacggagcaa accagcagat atttccaggt agcctccagg
     5701 cttttccagt tccggtgaaa gctgatcaaa ggcgcgctga aaagcgtggt cgtttcgatc
     5761 gccacccaca tcagcccgag atgatagctc accgacacca tcgtcgtcat cgaaagcagg
     5821 cacaggatgc aggccgtgaa cgtgcggttg ttccggtgct cgcgttcgaa ataaccaatc
     5881 atgaagaaag acgtcgccag gaaaagaagg tccaggatcg aaaggaggag gaggccgaga
     5941 ggatcgagca cgaaatagcc ctgaagttcc ggacccggcc gccgccacca gaaggacgcc
     6001 acgactgagg cgtggaagag cgaaaccgcg cacaggagcc ggaccctgaa gatgtttttc
     6061 gccggcaaca ggacaatcag agcggcgatc agcgggaccg cgatcagaag gatcagcgtc
     6121 atcagctctc cccccacctg ggccgccgcc ccgtggaacg ggtgcgttcg aactcctcgt
     6181 tgatatggtt gaggacgatg gccatgacaa agaccgcgac aaagatatcc agcaggatgc
     6241 ccatttcgat cggcaccggc atcgtgtcaa agagcgacag cgcgaaaaga aaaatgccgt
     6301 tttcgatcac catgtagccc aggacctgcg tgaccgcgcg ggtccgcgtc accatcgtca
     6361 tgaaaccaag aaggatcgtc gccagcgaac aaggcaggac caggctcgac tcaagcttcc
     6421 cgggaagttt catcgcgagc gagatccaga aggccacggc gatcag
//
LOCUS       DAOOBX010000008         6065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_15181_length_6065_cov_3.092346, whole genome shotgun sequence.
ACCESSION   DAOOBX010000008 DAOOBX010000000
VERSION     DAOOBX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15181_length_6065_cov_3.092346"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PLY30_00255"
     CDS             <1..716
                     /locus_tag="PLY30_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPW64560.1"
                     /translation="PYFLKYCWNTVYLMLSIPLSMAGSLLLAIALNQKLKGTVIFRTV
                     YFLPTICSGVAIFMLWRLIYNANSGLLNIMIAQMGNFLGLKLVGPNWLTDEAWAKPAF
                     IIMSVWQGIGGYNMILYLAALQGVPRELYDAAEVDGANSWQKFWNVTWPQISPTTFFI
                     VIMSLIGGFQAGFDQAYIMTGGGPNGSTTTIIYYIYNNAFEWYRMGYAAAISWVLFLI
                     IFMITLLKWRFYGRNIHYS"
     gene            740..1663
                     /locus_tag="PLY30_00260"
     CDS             740..1663
                     /locus_tag="PLY30_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPW64561.1"
                     /translation="MKQKSYIARNPALLKKGTPFIEVKLNYPLKRLFQRSFIYVALLI
                     GAVSMIAPFLWMLSTSLKNPNDVLAFDKVWWKEWLPMSFVWQNYVKVWQMKTVPFAMF
                     YLNSLFVSICITVGQVATSAMAAYAFARLSFPGRDKLFFGYLATMMIPGTVTMIPVFI
                     LLRYLGWIDTYKALILPGIFSAYGTFMLRQFFLTLPKDLEDAAKIDGCNYFRIFWHII
                     IPLSKPALATLTTFTFMGSWTTLMWPLIVVNTHTKYTLPVGLAYLQGVHNTDWTLLMA
                     GSMMMILPILVVFLFNQRFFVEGIKLTGIKG"
     gene            1667..2917
                     /locus_tag="PLY30_00265"
     CDS             1667..2917
                     /locus_tag="PLY30_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPW64562.1"
                     /translation="MDAVVLAAGKGTRMRTELPKVLHTAFGKPVLGYVLDTLAAAGIR
                     KPLVVVGYKAGLVKSFLKNYDARPVLQTEQKGTGHAVMMTRSLLKGSKGPVLIWPGDM
                     PLVETGTIERFVAANARSRAHASVLSSVVECPKGYGRIVREDGKFVAIREELDATDRE
                     RSLREVNTGIYLFDRELLFRALARIGSNNRKNEYYLTDTIEVLREEGCKVEAFPFAAA
                     AEGTGINSQKDLATVTERINQREIERHMERGVTFVSPARTFVAPGVVIGAGTVVNPWC
                     WVETGVTIGKNCKIGPFAKLRPGTKVGDGSEIGSFVEVNRSTVGKGVCAKHLSYLGDA
                     VIGDGVNIGAGAITANYDGKNKHVTKIGKNALIGSNTVLVAPLVVPERVKTGAGAVVT
                     RGTRMKKGGVVVGIPARQISKKKR"
     gene            2925..3914
                     /locus_tag="PLY30_00270"
     CDS             2925..3914
                     /locus_tag="PLY30_00270"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPW64563.1"
                     /translation="MTKIRVHKKNNGSMVLIGGSSNPALTAEIAKLLGIPVGDVVIGR
                     FPEGEIQVQIRDNIRGKDVFIVQPTCTPPNESLMELLILIDAARRASAKRITAVLPFF
                     GYARQDRKDRPRVPITAKLVANLIVSAGANRVLTVDLHAGQIQGFFDIPVDHLYSIAV
                     LGNYFKKKKLKNLVVVSPDVGGIKMARGYAKLFGCDLAIVDKRREGAATTHVMHIIGS
                     VTGKNVVLIDDLISTGGSIVEAAKALKARGVKDIYACIVHPVLAGSAVEKIRNSPIKE
                     LIVTNSIPLSEEKRIPKIKQLSIAPLLADAIWRIHNDVSISTLFFNSSLGVEA"
     gene            4012..4710
                     /locus_tag="PLY30_00275"
     CDS             4012..4710
                     /locus_tag="PLY30_00275"
                     /inference="COORDINATES: protein motif:HMM:NF004128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPW64564.1"
                     /translation="MQTYELKVTVRQVEGSRKARSVRRNEMIPAIVYGHGMKPLALEL
                     PEREFLKAIRTKAGENVMISLKVEGVQLKESICLIKDMQRNPMTEKIDHVDLMVISMT
                     EKITVKVPVVVVHADLAPGVKEGGVLDVVHHEVEVECLPTQIPEKIEIDAKEMKINDA
                     IHVSALALAEGVECLLSPDDVVVTVHPPREEEVAQPAEEAATQPEVIEKGKKPAEGEE
                     GAAPAAKPGAAPAK"
     gene            4767..5369
                     /gene="pth"
                     /locus_tag="PLY30_00280"
     CDS             4767..5369
                     /gene="pth"
                     /locus_tag="PLY30_00280"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPW64565.1"
                     /translation="MKLIVGLGNPGTSYAGTRHNIGRRLIEHIAAREGGSFARKPSLR
                     ACAAEVSWDCEPVVIAYPESYMNLSGSPVKLLAEHFGVRDYKNLLIVVDDIALPFGRF
                     RLRGDGSPGGHNGLASIEDTLRSAAYARLRVGIGVRREDDPNRSAGADEPLRDYVLAA
                     FSRAEEKEIPGLLEQGAEACRRWAVEPFAKAAHWVNSTKL"
     gene            5511..5858
                     /gene="rpsF"
                     /locus_tag="PLY30_00285"
     CDS             5511..5858
                     /gene="rpsF"
                     /locus_tag="PLY30_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPW64566.1"
                     /translation="MRKYEGLFIFPTEATGETHKDGSAEVARWIEKFQGRVLQKTDLG
                     KKTIGHPVGKHKDGKVLVVQFEMDPAKMQDFHKELEFQENLLKYMITVPDERKAKTPV
                     AAAPVAQPVTPQT"
     gene            5876..>6065
                     /gene="ssb"
                     /locus_tag="PLY30_00290"
     CDS             5876..>6065
                     /gene="ssb"
                     /locus_tag="PLY30_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPW64567.1"
                     /translation="MAANLNKVMLIGNLTRDPELRYLPSGQAVATFTIACGRTYMART
                     GEKKEEVSFIRIVVWARLA"
BASE COUNT         1278 a         1715 c         1888 g         1184 t
ORIGIN      
        1 atccttactt tctgaaatat tgctggaaca cggtctacct gatgctctcg atcccgctca
       61 gcatggccgg ttcgcttctt ctcgcgatcg cgctcaacca gaaactcaag gggacggtga
      121 tcttccgcac ggtctatttc ctgccgacga tctgctccgg cgtcgcgatc ttcatgctct
      181 ggcgactgat ctacaacgcc aactcgggtc ttctcaatat catgatcgcg cagatgggga
      241 acttcctcgg cctcaagctc gttgggccca actggctgac ggacgaggcc tgggccaagc
      301 cggcgttcat catcatgagc gtctggcagg gaatcggcgg ttacaacatg atcctgtacc
      361 ttgcggccct gcagggtgtc cccagggagc tctacgacgc ggccgaggtg gacggcgcga
      421 acagctggca gaagttctgg aacgtgacct ggccgcagat ttccccgacg acgttcttca
      481 tcgtgattat gagcctgatc ggcgggttcc aggcggggtt cgaccaggcc tatatcatga
      541 caggcggcgg gccgaacggt tccacgacga cgatcattta ctacatttac aacaacgcgt
      601 tcgaatggta ccgcatggga tacgccgcag cgatctcgtg ggtgctcttc ctgatcatct
      661 tcatgattac gcttctgaaa tggcgttttt acggacggaa catccactac tcctgaggcc
      721 gcggaaactt atgcccggaa tgaaacaaaa atcatacatc gccaggaatc cggcgctcct
      781 gaaaaaggga acgccgttca tcgaggtcaa gctgaactat ccgctcaagc ggctctttca
      841 gcggtccttc atctatgtcg cgttgctgat cggcgccgtc agcatgatcg cccctttcct
      901 ctggatgctt tcgacctcgc tcaagaatcc gaacgatgtg ctggccttcg acaaggtgtg
      961 gtggaaggaa tggctcccca tgagcttcgt ctggcagaat tacgtgaagg tctggcagat
     1021 gaagaccgtg ccgttcgcga tgttctacct gaactcgctt tttgtgagca tctgcatcac
     1081 ggttggccag gtggcgacga gtgcgatggc ggcctacgcg ttcgcgcggc tgagttttcc
     1141 cgggcgcgac aaactgttct tcggttatct cgcgacgatg atgatccccg ggacggtgac
     1201 gatgatcccc gtgttcatcc tgctgaggta cctcggctgg attgacacgt acaaggcgct
     1261 gatccttccg ggtatttttt cggcgtacgg aacattcatg ctccgccagt tcttcctgac
     1321 gctgccgaag gacctcgagg acgcagcgaa gatcgacggt tgcaactatt tcaggatctt
     1381 ctggcacatc atcatcccgc tctcgaagcc cgcgctcgcg acgctgacga ccttcacgtt
     1441 catggggtcc tggacgacgc tgatgtggcc cctgatcgtt gtgaacacgc acacgaaata
     1501 caccctgccg gtcggactgg cctacctgca gggggtccac aacacggact ggacgctcct
     1561 gatggcgggc tcgatgatga tgatcctgcc gatcctcgtg gtgttcctgt tcaatcaacg
     1621 gttcttcgtc gagggcatca agctgacggg gatcaagggc tgagccatgg atgcggtcgt
     1681 gctggcggcg ggaaaaggga cgcggatgcg gacagagctt cccaaggtgc tgcatacggc
     1741 cttcgggaaa ccggtgttgg gatatgtgct ggacacgctg gcggcggccg gaatccggaa
     1801 gccgcttgtg gtggtcggat acaaggccgg ccttgtcaaa agcttcctga aaaactatga
     1861 tgcacggccc gtcctgcaga ccgagcagaa ggggaccgga cacgcggtga tgatgacgcg
     1921 ttcccttttg aagggttcga agggccccgt gctcatctgg ccgggggata tgccgctggt
     1981 cgagaccggg acgatcgaaa ggttcgtcgc ggcgaacgca cggagccggg cccacgcgag
     2041 cgtgctgagt tccgtcgtgg aatgcccgaa gggttacgga cggatcgtcc gcgaggacgg
     2101 gaagtttgtc gcgatccgcg aggagttgga cgctacggac agggaacggt ccctccgcga
     2161 ggtcaacacg gggatttacc ttttcgaccg ggagctcctt ttcagggccc tcgcgcggat
     2221 cggcagtaat aacaggaaga acgaatacta tcttacggac acgatcgaag tcctccgcga
     2281 ggaaggctgc aaggtggagg cgttcccgtt cgccgcggcg gcggaaggga cggggatcaa
     2341 ctcccagaag gacctggcga cggtaacgga aaggatcaat cagcgcgaga tcgaacgcca
     2401 catggaacgc ggcgtgacct tcgtttcccc ggcccggacg ttcgtggcgc cgggggtcgt
     2461 gatcggcgcg gggaccgtcg ttaatccgtg gtgctgggtc gagaccggcg tgacgatcgg
     2521 aaaaaactgc aagatcggtc cgttcgcgaa gctccgcccg ggcacgaagg tcggggacgg
     2581 ttccgagatc ggcagtttcg tcgaggtaaa ccgctcgacg gtcggcaagg gcgtgtgcgc
     2641 gaaacatctg tcgtatctcg gcgacgcggt tatcggcgac ggtgtgaaca tcggggccgg
     2701 tgcgatcacg gccaactatg acggaaagaa caaacatgtc acgaagatcg ggaagaacgc
     2761 gctgatcggc tcgaataccg ttctcgtggc gcccctggtt gttcccgagc gggtaaagac
     2821 cggcgccggg gccgtcgtga cacggggcac acgcatgaaa aaaggtgggg tcgtggtcgg
     2881 gatccccgcg aggcagatct caaaaaagaa aaggtgatga ctttatgacg aagatcagag
     2941 tacacaaaaa gaacaacggt tccatggtcc tcatcggggg aagctccaac cccgcgctga
     3001 cggccgagat cgcgaagctc ctcgggattc ccgtgggcga tgtggtgatc ggaaggttcc
     3061 cggaaggcga gatccaggtc cagattcgcg acaatatacg cggcaaggat gtctttatcg
     3121 tgcagccgac ctgcacccct ccgaacgaga gtctgatgga gcttctgatc ctgatcgacg
     3181 ccgcccgccg cgcgtccgcg aagcgcatta cggcggtgct gccgttcttc ggttacgccc
     3241 gccaggaccg caaggaccgg ccccgcgttc cgatcacggc gaagctggtc gcgaacctga
     3301 tcgtgagcgc cggcgcgaat cgcgtcctca cggtcgatct ccatgccgga cagatccagg
     3361 gcttcttcga tatccctgtc gatcaccttt attcgatcgc cgtgctcggc aactacttca
     3421 agaaaaagaa gctgaagaac ctcgtggtcg tgtcacccga cgtcggcggc atcaagatgg
     3481 cccgcggtta tgcgaagctt ttcggctgcg acctcgcgat cgtggacaaa cgccgcgaag
     3541 gcgccgcgac gacgcatgtc atgcacatca tcggttccgt gacgggcaag aacgtcgtcc
     3601 tgatcgatga cctgatttcg accggcggtt cgatcgtgga ggccgcgaag gcgctgaagg
     3661 cgcgcggggt caaggatatc tacgcgtgca tcgtgcatcc cgtgctcgcg ggaagcgccg
     3721 tggagaagat caggaactcc ccgatcaagg aactgatcgt gacgaacagc atccctctct
     3781 ctgaagagaa gaggattccg aagatcaagc agctttcgat cgccccgctt ctcgcggacg
     3841 cgatctggag aatccataac gacgtttcga tcagcacgct ttttttcaac agttctctcg
     3901 gcgtcgaggc ctgagcgggg ccccgatagc ggagtttgac cgttttagac cctaaaaaca
     3961 aaccaccggc agaggcagcg aagtcttcgc cggttcaagg aggatccatc catgcaaacg
     4021 tatgaactga aagttaccgt ccgtcaggtc gagggcagcc ggaaggcgcg tagcgtccgc
     4081 cgcaacgaaa tgatcccggc cattgtctac ggccacggca tgaagccgct cgcgctggag
     4141 cttccggaac gcgagttcct gaaggcgatc cgtaccaagg cgggcgagaa cgtcatgatc
     4201 tcgcttaagg tcgagggtgt ccagctcaag gaatcgatct gcctgatcaa ggacatgcag
     4261 cgcaatccca tgaccgaaaa gatcgaccac gtcgatctga tggttatttc gatgaccgag
     4321 aagatcacgg tcaaggtgcc ggtcgtcgtg gttcacgcgg acctcgctcc cggcgtcaag
     4381 gaaggcggcg tgctcgatgt ggtccaccat gaagtcgagg tcgagtgttt gccgacgcag
     4441 attccggaga agatcgagat cgacgcgaaa gaaatgaaga tcaatgacgc gatccacgtc
     4501 agcgcgctcg cgctcgccga aggcgtcgaa tgtctcctgt ctcccgacga tgtcgtcgtg
     4561 accgttcatc ctccccgcga ggaagaggtc gcgcaaccgg cggaagaagc cgcgacgcag
     4621 ccggaagtca tcgaaaaggg caagaagcct gcggagggcg aggaaggcgc cgctcccgcg
     4681 gcgaaacccg gcgcggcccc ggcaaaataa ggaaagtccc ggagaccggc ggacgccttg
     4741 cgcgttcgcc ggtctttttc ctgtctatga aacttatcgt cggcctcggc aaccccggaa
     4801 cctcctatgc gggaacgcgg cacaatatcg gacgccgttt gatcgagcat atcgctgccc
     4861 gggaaggcgg gtctttcgcg aggaaacctt ccctgagggc ctgcgccgcc gaagtttcct
     4921 gggactgcga acccgttgtg atcgcctatc ccgaaagtta tatgaacctc tcgggtagtc
     4981 cggtgaaatt gcttgcggag catttcgggg tgcgggacta taaaaatctt ttgatcgtgg
     5041 tagacgatat cgcgcttccg ttcgggcggt tccgtctgag aggggacggc agccccggtg
     5101 gccataatgg cctcgcgtcc atcgaggaca ctctccggag cgccgcctac gcgcgattga
     5161 gggttgggat cggcgttcgg cgcgaggacg acccgaaccg ctccgcaggg gcggacgaac
     5221 ctctccgtga ctatgtcctg gccgcattca gtcgcgcgga ggaaaaagag attcccgggc
     5281 tcctcgaaca gggggccgag gcctgccggc gatgggccgt cgagccgttc gcgaaggcag
     5341 cccactgggt caattcgaca aaattgtagg ttatcttttg ttgcggaaca gagggcttta
     5401 tgttacattg tcactccttt cagggccttc tggaggttcc cagctgattt gccgcacgga
     5461 acgccttagc taaggtaacc gatcgtctac aggaataaga ggagtcaaga atgagaaagt
     5521 atgagggttt gtttattttt ccgacggaag ccaccgggga gacccataag gacggctctg
     5581 cggaagtggc caggtggatt gaaaagttcc aggggagggt cctccagaag accgatcttg
     5641 gaaagaaaac catcggtcat cccgtcggga agcacaagga cgggaaggtg ttggtggtcc
     5701 agttcgagat ggaccccgcg aagatgcagg atttccacaa ggaactcgaa ttccaggaaa
     5761 acctcctgaa gtacatgatc acggtccccg acgaaaggaa ggccaaaacg cctgtcgcgg
     5821 cggccccggt cgcgcaaccg gtcactcccc aaacctgaac acctgaggtg atgtgatggc
     5881 ggcaaatctg aacaaggtca tgctgatcgg gaatcttacc cgtgacccgg aactccggta
     5941 tcttccttcc gggcaggccg tggccacctt cacgatcgcc tgcgggcgga cctatatggc
     6001 caggaccggc gaaaagaagg aagaggtcag tttcattcgc atcgtggtct gggcgcggct
     6061 ggccg
//
LOCUS       DAOOBX010000009         6052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_15219_length_6052_cov_3.355845, whole genome shotgun sequence.
ACCESSION   DAOOBX010000009 DAOOBX010000000
VERSION     DAOOBX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15219_length_6052_cov_3.355845"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PLY30_00295"
     CDS             <1..1062
                     /locus_tag="PLY30_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64568.1"
                     /translation="YANLRSIDRGIGSRTLSTAIGSSFSLTEKSRIYSEREYSSYQGV
                     DGFADILGYEGALNDHWDYGAKYERRHLDSSMARNLLTLVENSLAQNNSSNVYGGYLA
                     YADGRKLRARTYAELRSNGAYPKGNQLVLRNSFQYQIHRELTFLTKFDYGATYLFDLV
                     DESKQTNLGNFADFTVAFAYRPIGLDHLNFLFKYSYLRDIGNDFQYVNSYYNENMFHE
                     IAHVISMDAIYDVNRFFGLAQKLAWKRATLITQDRDLFTLARNTLDETDTGNFLSVSR
                     INFHVTRKWDIAAEYRLLWQSSALDTIRHGMLFEVDREIYDYVRIGIGYNFTDFTDDL
                     RDANNYHNSGPFVRMTGKF"
     gene            1129..1518
                     /locus_tag="PLY30_00300"
     CDS             1129..1518
                     /locus_tag="PLY30_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64569.1"
                     /translation="MMKKILMCVAVLACLYLPGCSSVHKVLPVHDEVLLYNKPYDLTY
                     MNTLMALNAKPGWELESTDKEKGLITVRNTEYTRFDDADKRVISILVKRVDREQTSVS
                     LAPDSRQRLSSGEIIDWIGDRLPSDAR"
     gene            1535..4336
                     /gene="uvrA"
                     /locus_tag="PLY30_00305"
     CDS             1535..4336
                     /gene="uvrA"
                     /locus_tag="PLY30_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPW64570.1"
                     /translation="MDSIELRGVRTHNLKNLHLRLPHRKFYVVTGVSGSGKSSLAFDT
                     LYAEGQRRYLESVSAYARQFLERMPKPDLESATGIPPAIAIQAKNIVTNARSTVGTQT
                     EINDYLRVLFARAGRLTCPDCGKEVESHDPAGTADRLIADASGRKFRIVIRIAVGRSG
                     KKYLKENIAELERQGFGPFFHKGEWREGSAVVAAKPAPEELFVGIDELIPAANERERL
                     IDSLETGFRYGQGEVQAWTDGERLTFSEKLRCISCDRTFPEPTSNLFSFNSPLGACPV
                     CQGFGRVITIDRDLVIPDPSRSISGGAIEPWTKASTRWEQGQLLDFCSRKRIPVKKPW
                     AKLKKEHQEWIFNGREGDEYFSVRDFFDYLAKKIYKMHVRIFLNRYRGYIPCSACGTA
                     RLKPEAMNYRVGGKTIAEIQAMPVDRLLEFLRGIEFRPAEREKTGPVHEELLARVGFL
                     KEVGLGYLTLDRMSRTLSGGEVERIHLASSLGSALVDTLYVLDEPSVGLHERDNEMLI
                     NLLKKLRDLGNTVVVVEHDRTMIEAADEVIDLGPDGGAGGGRLIYQGPVRGLPGVKDS
                     HTARYLSGREKLTRAFRVPESGRAARNKTAAITIINARAHNLKNISVTVPLGRLTVLT
                     GVSGSGKSTLLYDVLYHHYLKSRGEPVSREELGEAGRVTGFEHIAKMIVIDQSPIGRS
                     PRSNPATYTKAFEEIRKLFAATQAARRAGFDAGHFSFNSEKGRCPACKGDGAVKVEMH
                     FLADVFVPCEACRGKRFKGEILEIEYQGKNIDQVLNMTVDEAAVFFADRKGLIEKLDI
                     LRRIGLGYLRLGQPTLTLSGGEAQRLKLASELTELGPEPSLYLLDEPTTGLHERDIHH
                     LLKAFDELLARGHSLFVIEHHMGLISLADHVIDLGPEGGDRGGEVLYAGDVPGLLACE
                     RSYTGRCLKKYNTGQGINV"
     gene            4407..4916
                     /locus_tag="PLY30_00310"
     CDS             4407..4916
                     /locus_tag="PLY30_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64571.1"
                     /translation="MSRKCLFLTLVIVLAVPNLAAIRRSEDPIRDTQTDGILYKQKME
                     EEKDGKKGALLYSVKYNPKDTFLTAPPFAPEAEKPAAREAMPGETAGLNWWDEPAVPA
                     DALAAPEEPVSPEQPAEGYWEQETAAGTEVLPGFDAAAEMTDAGGVSAGISPETAATN
                     AAPSEDYWW"
     gene            5078..>6052
                     /locus_tag="PLY30_00315"
     CDS             5078..>6052
                     /locus_tag="PLY30_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW64572.1"
                     /translation="MKSYRRWKLFWMDHWIKIAIVAASLILILLSIWGMASLESYYRN
                     ITLAQLPLQMIIMVINAVVFAYIYMVLFRGNLGNLEKKKNIAAKDVNVKFSDVIGIDE
                     AKEECWEVVQLIKDHARLKKIGGKILRGILMMGPPGCGKTYLAKAIATEAGLPFLSMA
                     ASEFNEIFVGVGASRVRQLFKKAARMAYGFGGCIVFIDELDAMGHRRTFSQLGSGEGN
                     TTQNQLLVAMDGLGEKTENIVVIGATNAAEDVLDPALLRPGRFDRKVYIKKPNHEGRE
                     ALFKFYLGKVKHEASVDCKRLARKAIDKSPADIENIVKTFDGLTVTKGI"
BASE COUNT         1335 a         1698 c         1763 g         1256 t
ORIGIN      
        1 tatgcgaacc tccgctcgat cgaccgcggc attggttcga ggacactctc aaccgcgatc
       61 gggagctctt tttcgcttac cgagaaatcg cgaatttatt cggaacgcga gtactcctcc
      121 taccagggcg tggatggttt tgcggatatt ctcggctatg agggcgcgct gaacgaccac
      181 tgggattacg gcgcgaagta tgagcggcgg cacttggaca gttcgatggc ccggaatctg
      241 ctgacattgg ttgaaaatag cctggcgcag aataacagct ccaacgttta tggaggctat
      301 ctggcctacg cggacggcag aaaactccgt gcgcggactt atgcggaact gagaagcaat
      361 ggtgcgtatc ccaagggtaa tcagctggtc ttgcgcaatt catttcaata tcaaattcat
      421 cgcgagctga cttttctgac caaatttgat tatggcgcca cctatctttt cgatctcgtg
      481 gatgaaagca agcaaacaaa tcttgggaat tttgcggatt ttaccgtggc ttttgcctat
      541 cgccctattg gtcttgacca cctgaatttt ctctttaagt atagctattt gagagacatc
      601 ggaaatgatt ttcagtacgt gaattcttat tacaacgaaa acatgttcca tgagatcgcg
      661 catgttatct caatggacgc gatctatgac gtgaatcggt tcttcggtct cgcacagaaa
      721 ctggcgtgga aacgggcaac gctgatcacc caggatcgcg acttgttcac tttggcccgt
      781 aacacgcttg atgagacgga taccggtaat ttcttgtctg tcagccgcat taatttccat
      841 gtgacccgga aatgggatat cgcggccgaa tatcggctct tgtggcaatc ctccgcgctc
      901 gatacgatac ggcacgggat gcttttcgag gttgatcgtg aaatctatga ttacgtgcgg
      961 atcgggatcg gttataattt taccgatttt acggatgacc tccgggacgc gaacaattat
     1021 cataacagcg gtccgttcgt gcggatgacg gggaagttct agccctgtcc ggtgacggac
     1081 aaaaaagccg gcttcaaatc gagaccctgc ggggttaagg agatatccat gatgaaaaaa
     1141 atcctgatgt gtgttgccgt tctcgcgtgc ctctatcttc ccggctgttc gtcggtccac
     1201 aaggtcctcc cggtccacga cgaggtcctt ctttataaca agccttatga cctgacctac
     1261 atgaacaccc tgatggctct gaacgcgaag cccggatggg aactcgagtc caccgacaag
     1321 gaaaaaggcc tcatcacggt ccgcaacacc gagtacaccc gctttgacga cgctgacaaa
     1381 cgcgttatct cgatcctcgt caaacgggtc gaccgggaac agacctcggt gtctctcgcg
     1441 cccgactccc ggcagcggct cagcagcggc gagatcatcg actggatcgg cgacaggctc
     1501 ccgagcgacg ccaggtagtt cgtccgggat ttccttggat tccatcgagc tccgcggggt
     1561 caggacccac aatctcaaga acctccacct caggcttccg caccggaagt tctatgtcgt
     1621 aacgggtgtc tccggatcgg gaaaatcatc ccttgccttt gacaccctgt atgcggaggg
     1681 acagcgccgc tatctcgagt ccgtttccgc gtatgcccgc caattcctcg agcggatgcc
     1741 gaagccggat ctcgaaagcg ccaccggaat ccctcccgcg atcgcgatcc aggccaagaa
     1801 tatcgtcaca aatgcccgct cgacggttgg cacccagacc gagatcaacg actatctccg
     1861 ggtccttttc gcccgggcgg ggcgcctgac ttgtccggat tgcgggaaag aggttgagag
     1921 ccacgacccg gcgggcacgg cggaccggct gatcgcggac gcttcgggcc gaaagttccg
     1981 gatcgtgatc cggatcgcgg tcgggagatc cgggaaaaaa tacctgaagg agaatatcgc
     2041 cgaactcgag cggcaagggt ttggcccgtt ctttcataaa ggcgaatggc gcgagggaag
     2101 tgcggtcgtc gcggccaagc cggcgcctga ggaacttttc gtggggattg acgaactgat
     2161 ccccgccgcg aatgaacggg aacgtctgat cgattcgctc gagaccgggt tccgttacgg
     2221 gcagggcgag gtccaggcat ggaccgatgg cgagcgattg accttttccg agaagctccg
     2281 ctgtatttcc tgcgaccgga cgttccccga acccacgtcc aaccttttct cgttcaacag
     2341 cccgctcggc gcctgccccg tctgccaggg gttcggacgc gtcatcacga tcgaccggga
     2401 cctcgtcatt cccgatcctt cgcgttcgat cagcggcggc gcgatcgagc cgtggacgaa
     2461 ggcgagcacc cgctgggagc aggggcaact gctcgatttc tgttcgagga aacgcattcc
     2521 tgtcaaaaag ccctgggcga aactcaaaaa agagcaccag gaatggattt ttaacggacg
     2581 cgaaggagac gaatacttca gcgtgcggga tttcttcgat tacctcgcga agaagatcta
     2641 caagatgcac gttcggattt tcctgaaccg ctaccgcggc tacattccgt gttcggcctg
     2701 cgggaccgcc cgtctgaaac cggaggcgat gaattaccgg gtcggcggca agacgatcgc
     2761 cgaaatccag gcgatgccgg tcgaccggct gctcgaattc cttcgcggaa tcgaattccg
     2821 gcctgcggaa agggagaaga ccggccccgt gcacgaggaa cttcttgcgc gtgtcggctt
     2881 tctgaaagag gtcggcctcg gctacctcac gcttgaccgg atgtcccgca ccctttcggg
     2941 cggggaagtg gagcggatcc atctcgcgag ctcgctcggt tcggcgcttg tcgacacgct
     3001 gtatgtgctg gacgagccga gcgtcgggtt gcacgagcgc gacaacgaaa tgctgatcaa
     3061 tctcctcaaa aaactgcgcg acctcgggaa tacggttgtt gtggtcgaac acgaccggac
     3121 gatgattgaa gccgccgacg aagtgatcga cctcggtccg gacggcggcg ccggaggagg
     3181 acggctcatt tatcagggac ctgtccgggg actccctggc gtgaaggact cccacacggc
     3241 ccgctatctt tcgggacggg aaaaactgac acgggcgttc cgcgttccgg aatccggccg
     3301 cgcggcccgg aataaaacag cggcgatcac gatcataaac gcgcgggcgc ataacctgaa
     3361 aaatatcagt gtgacggtcc cgctcggacg gctgacggtc ctgaccggcg tttcaggttc
     3421 cgggaaaagc acgctgctct atgatgttct ctatcatcat tatctgaaat cccgcggcga
     3481 acctgtttcc cgcgaagaac ttggcgaagc gggccgtgtc acgggattcg agcacatcgc
     3541 gaagatgatc gtgatcgacc agtcaccgat cggccgctct ccccggtcca atccagcgac
     3601 ctatacgaag gcgttcgaag agatccgcaa actctttgcc gcgacccagg cggcccggcg
     3661 cgcgggattc gatgccggtc acttttcctt caattccgag aaaggccgct gtccggcctg
     3721 caagggggat ggtgccgtga aggtggagat gcattttctc gcggatgtct ttgtcccctg
     3781 tgaagcctgc cgcgggaaac gtttcaaggg cgagatcctc gagatcgaat atcagggaaa
     3841 gaacatcgat caggtcctca atatgaccgt ggatgaagcg gccgtcttct tcgcggatcg
     3901 caagggcctc atcgagaaac tggatatcct ccgcaggatc ggcctcggct atctccggct
     3961 cggacagccg acgctcacgt tgtcgggagg ggaggcgcaa cgcctgaagc tcgcgtcgga
     4021 gcttaccgaa ttgggaccgg aaccatccct ctatctcctc gacgaaccga ccaccggtct
     4081 tcatgagcgg gatatccatc atttgctgaa ggcttttgac gagctcctcg cgcgcgggca
     4141 ttcgctcttt gtgatcgaac accatatggg actgatcagt ctcgcggacc atgtgatcga
     4201 tctcggaccc gaggggggcg acaggggtgg cgaggtcctc tacgcagggg atgtcccggg
     4261 tcttctcgcg tgcgagcggt cttatacggg gcggtgcctc aagaaataca acacagggca
     4321 gggtataaat gtttaagctc cgtattccgg acgccgcgcg atttgccagg accggttttt
     4381 tcagatactc tttacgtggt gcatccatga gcagaaaatg tctgttttta accttggtga
     4441 tcgtgcttgc cgtgccgaat cttgccgcga tccggaggag cgaggatccg atccgggaca
     4501 cgcaaacgga cggcattctc tataaacaga agatggaaga ggaaaaagac ggaaaaaaag
     4561 gagctcttct ttatagtgtt aaatacaatc ccaaagatac cttcctgacg gcgccgccgt
     4621 tcgctcccga ggccgaaaaa ccagcggcca gggaagccat gcccggggaa acggccggac
     4681 ttaactggtg ggatgaaccc gcggttcccg cggatgccct tgccgcgccg gaagagccgg
     4741 tttcccccga gcagcctgcg gaaggctatt gggaacagga gacagccgct ggaacggaag
     4801 ttctccccgg attcgatgcc gcagccgaga tgaccgatgc aggcggagtc tctgcgggga
     4861 tctccccgga aaccgcggcg acgaatgccg ctccctcgga agactactgg tggtgagact
     4921 gtcctttcct cccggagttt cgggttaaga caggggccaa agggcaaaat acctctataa
     4981 aacggaaata cttacggata taatacccca actatccgcc ttcccaggcc gctttcccaa
     5041 gtttgaaggc caagaatgaa aaggagaatt gtctgacatg aaatcatacc gtcgctggaa
     5101 gcttttttgg atggatcact ggatcaagat cgcgatcgtg gccgcctccc tcatcctgat
     5161 cctcctctcg atctggggga tggcgtcgct tgaaagctac taccgcaata tcacgctggc
     5221 gcagttgccg ctccagatga tcatcatggt catcaatgcg gtggtcttcg cctatattta
     5281 tatggtgctg ttccgcggca acctcgggaa cctcgagaaa aagaagaata tcgcggccaa
     5341 ggacgtgaac gtgaagtttt cggacgtgat cgggatcgac gaggccaaag aggagtgctg
     5401 ggaggtcgtt cagcttatca aggaccatgc gcgcctcaag aagatcggcg gcaagattct
     5461 tcgcggcatc ctgatgatgg ggcctcccgg gtgcggcaaa acatatctcg cgaaggctat
     5521 cgcgacggaa gcggggctgc cgtttctttc gatggccgcg agcgaattca acgagatttt
     5581 cgtcggcgtc ggcgcaagcc gcgttcgcca actcttcaag aaagccgccc gcatggccta
     5641 cgggttcggc ggctgcatcg tgtttattga tgagcttgac gcgatggggc accgccgcac
     5701 cttcagccag ttagggagcg gggaaggaaa caccacccaa aaccagctac tcgtcgcgat
     5761 ggatggtctc ggggaaaaga ccgagaacat cgtcgtgatc ggggccacga acgcggccga
     5821 ggacgtactc gatcccgcgc tgctccgtcc cggacgtttc gaccggaagg tctatatcaa
     5881 gaaaccgaat cacgagggcc gcgaggcgct cttcaagttc tatctgggca aggtcaaaca
     5941 cgaggcctcg gttgactgca agcgtctcgc gcgcaaggcg atcgacaagt cgccggccga
     6001 cattgagaac atcgtcaaga catttgatgg cctcaccgtc accaagggca tc
//
LOCUS       DAOOBX010000010         5956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_15493_length_5956_cov_2.666836, whole genome shotgun sequence.
ACCESSION   DAOOBX010000010 DAOOBX010000000
VERSION     DAOOBX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15493_length_5956_cov_2.666836"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLY30_00320"
     CDS             <1..265
                     /locus_tag="PLY30_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPW64573.1"
                     /translation="FATVADEEGFPEVARSFKLVAMVEKRHEDRYVKLADALEHHKIF
                     VKDGKVFWKCLNCGYIVEATRAPERCPTCDHAQEFFEMWVENY"
     gene            305..1312
                     /locus_tag="PLY30_00325"
     CDS             305..1312
                     /locus_tag="PLY30_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPW64574.1"
                     /translation="MPSYKELGLVNTREMFANAMKGGYAIPAYNFNNMEQIQAIVMSC
                     VETQSPVILQVSSGARKYANQTLLEYMGKGAVEMMKAYAKEKGLKPIPMALHLDHGDT
                     FELAKSCIESGFSSVMIDGSHHPFEDNIRLTRQVVEFAHRHDVTVEGELGVLAGIEDD
                     VSAEHSTYTDPNDVVKFVKETGVDSLAISIGTSHGANKFKPAQCTRNADGVLIPPPLR
                     FDILREIEQKIPGFPIVLHGASSVPQDAVRIINENGGALKDSIGIPEEQLRQAAKSAV
                     CKVNIDSDGRLWMTAAIRKVFREKPAEFDPRKYLGPARDALVAMYKHKNEKVLGSAGK
                     A"
     gene            1334..1660
                     /locus_tag="PLY30_00330"
     CDS             1334..1660
                     /locus_tag="PLY30_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64575.1"
                     /translation="MKKVLLLSLAVLLVGISPVRAEETGEVAGDMPGVSSLARMCAEG
                     GAGCCSAGSSMVAAQDGGVIVLSGEKLFKYDNALNLVKEVELPKPLQPEAPEAVNPDI
                     RASEPL"
     gene            1721..2965
                     /locus_tag="PLY30_00335"
     CDS             1721..2965
                     /locus_tag="PLY30_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64576.1"
                     /translation="MMKRVAVLSLCLILACPGIVLAELSWKDLGLPATRFPQTTQYYQ
                     VHTVDEDPSAPGFLIFSVSSEKVKTRVEGLADLKKWSHEAAVVAELDKDKNAAGGIAD
                     GAVESVKGTGRGLKNLVLHPVNSVKGIGTAAGRVGTKVGDAFREKEEGEKGDSFLSSG
                     KRQIAKKLGVDVYSRNEVLQEKLSTLAKQEMGGRGIVMVAKFFIPVGLVASAVMTASN
                     INNAADGLVNDRDRADLYSLNKKALIECGFEPGKAVAFLNHGYYTPREITYFRFYFEK
                     LSRLRGYKALLDEATRLSDAVPARKFLHEVQIAADAADTMPDAVAVDIVPEGLLIERK
                     NAAVLICGYDYLDASPLGDRIIKKADEYKWKLKKSSAEIWNAGIVTPRFGASLLVTGI
                     KARRMCLFRDNNNNATVDGPAI"
     gene            2983..3936
                     /locus_tag="PLY30_00340"
     CDS             2983..3936
                     /locus_tag="PLY30_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW64577.1"
                     /translation="MKHQTCLVTGGAGFIGSHIVDALVRKGFRVRVLDDFSRGKMENL
                     ELSRRHVKVIEGDVRSLKTVSAAVKGAACVFHLAAIASVPQSCADPLEAHEVNVTGTL
                     NVLLAARDAGVKRVVFTSSSAIYGDTRKFPTREDERPMPESPYAASKIMGEYYCRNFT
                     RLYGLETVSLRYFNVFGPRQDPKSQYSNVIPIFIRKMKRGETVTVHWDGKQSRDFVHI
                     DNVVSANLIAMRKPGVAGESFNVGCFEEKSILEIVRDLKACLGIRNVVTEFGPKRAGD
                     VRRTLADISKAKKKLGYRPVMFFKKGLQSTVRWFLDHPEAL"
     gene            4110..4691
                     /locus_tag="PLY30_00345"
     CDS             4110..4691
                     /locus_tag="PLY30_00345"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPW64578.1"
                     /translation="MNYLIVYSHPNPASFNHAIKETVVKALKGKGKNVRVRDVYSMRF
                     DPVLKADDFETFQQERTPRDIEIEQEHVRWADVLIFIYPVWWAGMPAIARGYIDRVFS
                     IGFAYENTPEGPKGLLTGKKVYVFNTMGAPVAAYEQSGIFKAIELLTDQETFRFCGLE
                     VLGHKYFGSVTTVTDDDRKAMLGEVERVAASLE"
     gene            4703..>5956
                     /locus_tag="PLY30_00350"
     CDS             4703..>5956
                     /locus_tag="PLY30_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64579.1"
                     /translation="MGPASFVGSYRITGNKVIIRLRNQICDSAEELLESGLCLEIVKR
                     AVAALQKRGSILLGIFGGRVVDDAGISTLMQVLQFLEKMDADRVACVVPGSEDFFRDR
                     NLLNDFVEYLYNFWRGFERFIVCDSEGDQLDRRPYRTFNRTIENLTSLVRHTYREIQE
                     NISGTHPRIYRQIAAGAEMATIATPKAIPYPGGAYQKLAPLSVVRQILFYPPLVLDPP
                     NNKRTGKFERIQENPLEWCNFSRDEWLCYPVKAGDTLVHVYFSEKFFELGLSMANLFE
                     LASDEDLKRKPDAVYLYGLSDDQFRAPEDVFPTVFFDDEANGILVGAVPARDEFGYFG
                     YLKKMIMTLHNIKKIQSGRMPFHGAFMKIHLKGKKPATLLLMGDTGAGKSETVEAMRG
                     FGADLIQDAIVIADDMGSLELKEDGT"
BASE COUNT         1275 a         1686 c         1801 g         1194 t
ORIGIN      
        1 gttcgcgacg gtcgcggacg aggaagggtt cccggaggtc gcaaggagct tcaaactggt
       61 ggccatggtc gagaaacgcc acgaggaccg ctacgtcaag cttgccgacg ccctcgaaca
      121 tcacaagatc ttcgtcaagg acggcaaggt tttctggaag tgcctgaact gcggttacat
      181 cgtggaggcg accagggccc ccgaacggtg tcccacctgc gaccacgccc aggaattctt
      241 cgaaatgtgg gtcgaaaatt actgatcttt caatcctcta actcattccc aaggagaaac
      301 ggcaatgccc agctataagg aactcggttt agttaataca cgtgagatgt tcgcgaacgc
      361 catgaagggc ggctacgcca ttcccgccta caatttcaac aacatggaac agatccaggc
      421 gatcgtgatg tcctgtgtcg aaacccagtc tcccgtgatc ctccaggtct ccagcggcgc
      481 gcgcaagtat gcgaaccaga ccctccttga gtacatgggc aaaggcgcgg tcgaaatgat
      541 gaaggcctac gcgaaggaaa agggcctcaa gccgatcccg atggcgctgc accttgacca
      601 cggtgacacc ttcgagctcg cgaaatcctg catcgaatcc ggtttttcgt ccgtcatgat
      661 cgatggttcc caccatccct ttgaagacaa tatcaggctg acccgtcagg tcgtcgagtt
      721 cgcgcacagg catgacgtga cggtggaagg cgaactcggc gtgctggccg ggatcgagga
      781 tgatgtgagc gccgagcact cgacctatac ggacccgaat gacgtcgtga agttcgtgaa
      841 ggagaccggc gtggattctc tcgcgatctc catcgggact tcgcatggcg ccaacaagtt
      901 caagccggcc cagtgcacga ggaacgctga cggcgttctg atcccgccgc cgctgcgttt
      961 tgacatcctg agagagatcg agcagaaaat ccccggtttt ccgatcgtgc tgcacggcgc
     1021 ttcctcggtg ccgcaggacg ccgtaaggat catcaacgag aacggcggcg cgctcaagga
     1081 ttccatcggg attccggagg aacagctccg ccaggccgcg aaatcagccg tctgcaaggt
     1141 caatatcgat tccgacggac gtctctggat gaccgccgcg atccgcaagg ttttcaggga
     1201 aaagcctgcg gaatttgatc cccgtaaata cctcggcccg gcgcgcgacg cgctggtggc
     1261 gatgtacaaa cacaagaacg aaaaggtgct tggctcggcc ggcaaggcct gactccccga
     1321 agggagaccc ggtatgaaga aggtcctttt gctgtcgttg gcggtgttgc tggtgggtat
     1381 atcaccagtc cgcgcggagg agaccgggga ggttgccggc gatatgccgg gtgtgtcctc
     1441 gcttgccagg atgtgcgcgg agggtggtgc gggatgctgc tctgcggggt cttccatggt
     1501 cgcggcacag gatggcggcg tgatcgtgct ttcgggtgaa aaacttttca agtatgacaa
     1561 cgcgctcaac ctggtcaagg aagtcgagtt gccgaagcct cttcagccgg aagcgcccga
     1621 agccgtgaac cccgacatcc gggcttcgga accgctttag gccggataca ttgaaccggg
     1681 aacccgattc ctgtaacgga aatcgcggga ggttgtcacg atgatgaaac gcgttgcggt
     1741 cctgtccctt tgtttgatcc ttgcatgccc cggcattgtc ctcgcggaac tcagctggaa
     1801 agatctcggc cttccggcga cccggttccc gcaaaccacc cagtattatc aggtccatac
     1861 cgttgatgag gatccgtccg ctcccggctt cctgatcttt tccgtctctt ccgaaaaggt
     1921 caagacccgc gtggaagggc tcgcggacct caagaaatgg tcgcacgaag ccgccgtggt
     1981 ggccgagctt gacaaggaca agaacgcggc cggcggcatc gcggacggag cggtcgaatc
     2041 cgtcaaagga acgggccggg ggctaaagaa cctggttctg caccccgtca attccgtgaa
     2101 gggtatcgga acggcggcgg gcagggtcgg caccaaggtg ggggacgctt ttcgcgaaaa
     2161 ggaagaaggc gaaaaagggg acagctttct cagctccggg aagcggcaaa tcgcgaagaa
     2221 gctcggcgtc gacgtctatt cccgcaacga ggtccttcag gaaaaacttt caaccctcgc
     2281 caaacaggag atgggcgggc gcggtatcgt gatggtggcc aagtttttca taccggtcgg
     2341 attggtcgct tcggccgtca tgacggcgag caatatcaac aacgcggcag acggactcgt
     2401 gaatgacagg gaccgggccg acctctattc tctcaacaaa aaagccctga tcgaatgcgg
     2461 tttcgaaccc ggaaaggccg tcgcgttcct gaatcacggt tactatacgc cgcgtgagat
     2521 cacttatttc cggttttatt tcgagaaact ttcgcggctg cggggctaca aggcccttct
     2581 cgatgaagcg acccgtttga gcgacgcggt cccggcccgg aagttccttc atgaagtcca
     2641 gatcgccgcg gacgcggcgg acaccatgcc cgacgcggtc gccgtggata ttgttccgga
     2701 agggcttctg atcgaacgaa agaacgcggc ggtccttatt tgtggttatg actatcttga
     2761 cgcgtcaccg ctcggggaca ggattatcaa aaaagcggat gagtacaagt ggaagctcaa
     2821 aaaatcctcc gcggagatat ggaacgccgg catcgtgacc ccgcggttcg gagcgtcact
     2881 gcttgtgacc ggcatcaagg cccggcggat gtgtcttttc agggacaaca acaataacgc
     2941 gacggtcgac ggcccggcga tttgaagcct caaaggagat ccatgaaaca ccagacatgt
     3001 cttgtcaccg ggggcgcggg gttcatcggc tcccatatcg ttgacgccct tgtccggaaa
     3061 ggatttcgcg ttcgcgtgct ggacgatttc tcccggggta aaatggagaa cctggagctt
     3121 tcgagacgtc atgtcaaggt gatcgagggc gatgtccgct ccctcaaaac ggtttccgct
     3181 gcggtaaaag gggccgcctg tgttttccac ctggccgcga tcgcgtcggt gccccagtcc
     3241 tgcgcggatc cccttgaggc ccatgaggtc aacgtgaccg ggaccctgaa cgtgctcctg
     3301 gccgcgcgcg acgccggggt gaagcgggtg gtcttcacgt cttcctccgc catttacggc
     3361 gacacccgga agtttccgac gcgcgaggac gaacggccga tgccggaatc cccgtacgcc
     3421 gcctccaaga tcatggggga atattactgc cggaatttca cgcggctgta cggcctcgaa
     3481 accgtgtcgc tccggtattt caacgtgttc ggtccgcgcc aggacccgaa atcccaatac
     3541 tcgaacgtga ttccgatctt catccggaag atgaagcgtg gcgagacggt tacggttcat
     3601 tgggacggga agcagtcccg ggatttcgtc catatcgaca acgttgtgag cgccaatctt
     3661 atcgccatgc gcaaacccgg tgtcgcgggg gagtcgttca atgtcggttg ttttgaggaa
     3721 aaaagcatcc tcgagatcgt aagggacctc aaggcctgcc tcgggatcag gaatgtcgtg
     3781 acggaattcg gaccgaaacg tgcgggcgat gtgcggcgga cgctggccga tatctcgaaa
     3841 gcgaagaaga aacttggtta ccggcccgtg atgttcttca agaaagggct tcagtccacg
     3901 gtccgctggt tcctggacca tcccgaagcg ctctgaccgg gagccggccg ccgggacttc
     3961 cgctttggct atccaatact tctaaaatcg gttataattc cctaactccc cgaagcccgg
     4021 aagattttcc gggatcccaa ggaaatgttc atcggagaag tgttccccgg aacgttttga
     4081 ttttcacacg gacccgaaag gagtcaaaaa tgaactattt gatcgtctac agtcatccga
     4141 atcccgccag ttttaatcac gcgatcaagg aaaccgtcgt caaggcgctc aagggcaagg
     4201 gaaagaacgt ccgcgtccgg gatgtttact cgatgcgttt cgatcccgtc ctgaaggcgg
     4261 atgatttcga gaccttccag caggaacgga caccgcgcga tatcgagatc gaacaggaac
     4321 acgtccgctg ggcggacgtc ctgatcttca tctatcccgt ctggtgggcg ggaatgcccg
     4381 cgatcgcgcg cggctacatt gaccgcgttt tcagcatcgg gttcgcgtat gagaacaccc
     4441 cggaaggacc gaaggggctt ttgaccggga agaaggtcta cgtcttcaac acgatgggcg
     4501 cgccggtcgc ggcgtacgag caaagcggca tcttcaaggc gatcgaactg ctgaccgacc
     4561 aggagacctt caggttctgc gggctcgagg tcctcggcca caaatatttc ggttccgtga
     4621 cgaccgtcac cgatgacgac cgcaaggcga tgctcggcga agtggaacgc gttgcggcgt
     4681 cgctcgagta aaggggattt ccatggggcc agcctctttt gttggttctt accggatcac
     4741 cggtaacaag gtcatcatcc ggctccgcaa ccagatttgt gactcggccg aagaacttct
     4801 cgagagcggc ctgtgcctcg agatcgtgaa acgggccgtt gcggcgctcc agaaacgcgg
     4861 ttcgatcctt ctcggtattt ttggggggcg tgtggtcgat gacgccggga tttcaactct
     4921 gatgcaagtc ctccagttcc tcgaaaaaat ggacgccgac cgtgtcgcgt gtgtggtgcc
     4981 gggctcggaa gactttttcc gggaccggaa cctcctgaac gattttgttg aataccttta
     5041 caatttctgg cgcggtttcg agcggttcat cgtctgtgat tcggaaggcg accagctgga
     5101 caggcgcccg taccggacct tcaaccggac gatcgagaat ctgacgtcgc ttgtccgcca
     5161 tacctaccgt gagatccagg agaatatcag cggcacgcac ccgcgcattt accgtcagat
     5221 cgccgccggc gcggagatgg ccacgatcgc gacgcccaag gcgatcccgt acccgggggg
     5281 cgcgtatcag aagctcgcgc cgctcagcgt ggtccggcag attcttttct atccgccgct
     5341 cgtgctcgat ccgccgaata acaagcgcac cgggaagttc gagcggatcc aggagaaccc
     5401 gctcgagtgg tgcaatttct cccgcgacga gtggctctgc tatcccgtga aggcggggga
     5461 cacgctggtt cacgtctatt tcagcgaaaa attcttcgag ctcggccttt cgatggcaaa
     5521 ccttttcgag ctcgcgtccg atgaggacct gaagcgcaag cccgacgccg tctacctcta
     5581 cgggctttcg gacgaccagt tccgggcccc ggaggacgtt ttcccgacgg tcttcttcga
     5641 tgacgaggcg aacgggattc tggtcggcgc ggtcccggcc cgggacgagt tcggctattt
     5701 tggttacctg aaaaagatga ttatgaccct tcacaacatc aagaaaatcc agtcgggacg
     5761 gatgcccttt cacggcgcgt tcatgaagat ccatctcaaa gggaaaaagc ccgcgacgct
     5821 tctcctgatg ggggatacgg gcgccgggaa atccgaaacg gtcgaggcga tgcgcggttt
     5881 cggcgccgac ctgatccagg acgcgatcgt gatcgcggat gacatggggt cgctggaact
     5941 gaaagaagac gggacg
//
LOCUS       DAOOBX010000011         5941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_15545_length_5941_cov_2.504077, whole genome shotgun sequence.
ACCESSION   DAOOBX010000011 DAOOBX010000000
VERSION     DAOOBX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15545_length_5941_cov_2.504077"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLY30_00355"
     CDS             complement(<1..741)
                     /locus_tag="PLY30_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HPW64580.1"
                     /translation="MCYTSGMKKFPDGFLWGAATAAHQVEGGNIGSDWWEWEQTRGRE
                     RSGDACRHYELYERDFDLARSLHHNAHRLSVEWARVEPQEGRFSDDALKHYAEVVLAL
                     KDRGIEPVVTLHHFTNPLWFARDGGWEDRRAPGRFLRYCDAVVRILGKHVRYWITINE
                     PTIYVSHSFLFGVWPPQARSLWRMWRVSRNMVRAHVAVFRMIHQVCRDFGLREPAVSI
                     AQHMTALVPCGSGLRCRFASWLRHKIFNE"
     gene            787..2073
                     /locus_tag="PLY30_00360"
     CDS             787..2073
                     /locus_tag="PLY30_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPW64581.1"
                     /translation="MRSLIGQIKKTYRMAGRHERRTVLENFASLSILQAITYILPVII
                     IPYLFRVIGPDKFGLIAFAQSFIQYFMILTDYGFGISATREISLYQDRRARISEIFSA
                     VITAKIVLAALSLMILGAIVYLVPRFRADWMVYLFSFGAVIGNTIFPVWLFQGTEKMK
                     HIADLNILGGVLFALFVFMFVKSPSDYLIVPLVYSVVSIMTGLWALFIGFTKLGISFR
                     FPGYTSMQQQMKAGWDIFISNVAINAYTTTRVFTVGLLTNNTLTGYYSIAERIAGFCQ
                     QFPLSSFSQALFPRLSKIFQKNQRRAYEFMRKIQQITVSISLICLPLIFLFAGPIVRI
                     ICGGDSPQAVLTLRILLCAVFIVGANAFRIQFLLICGKTSIYSRIHIVMAMVGLPLIF
                     LLIYSFSYAGAAIATVIIEVGIFTITYFTVKQLRLK"
     gene            2095..2970
                     /locus_tag="PLY30_00365"
     CDS             2095..2970
                     /locus_tag="PLY30_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64582.1"
                     /translation="MGKTIFPAMGISHEKHPLLFRSRRIFAAPVWPVAGTSGNAGVTI
                     AKNKRHSNVPDSPLRLGYNRSAMLTTSNLKIHPQVLRQAPARAIQFWGSFGSSVGNST
                     GISAGGSGSCHPPPLPGPFHIGDSLWANNIDLPLAFAKDRQPDVVSVRRNQITRLLSP
                     FDTANRSRIRRKIPHSDIGKFVDRIDPVTVKMIQGNLCPVHMHEDKGRALYLFGTWKP
                     KTCRDSLDKRCFPASEIPFEAKNTSRFQGQRKPFRERDRFIGRRCNGRQFLHDLKMPE
                     SFPRQPPPHPDPLVI"
     gene            complement(2908..3837)
                     /locus_tag="PLY30_00370"
     CDS             complement(2908..3837)
                     /locus_tag="PLY30_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPW64583.1"
                     /translation="MDEFTFIERLKKKIPPAKNAVLGIGDDAAVLRFSGPGRLVVSTD
                     MIVEKVDFVLNRSEPEQIGRKALAVNLSDLAAMGAKPLAFVISIGKPAYVDARWLDRF
                     YDGLLRLARRYKVDCVGGDFSAAKEFSASVTIFGTARKPILRSGAKAGDWIAVTGRLG
                     GSILRHHFDFTPRVREGLALARSGLVHSMIDISDGLCQDLHHILNASCVAAELDPRRI
                     PVSSDAIHLGRKNSLAALERALTDGEDFELLFTVPGPAKSVLERRWKKECRGVSLNWI
                     GRIVKGKPGIRWISNDKRVRMRRGLSRKGFRHF"
     gene            complement(3880..4944)
                     /gene="mtnA"
                     /locus_tag="PLY30_00375"
     CDS             complement(3880..4944)
                     /gene="mtnA"
                     /locus_tag="PLY30_00375"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPW64584.1"
                     /translation="MKNPKVPFSPVEFRKETLYLLDQRKLPNREVWVACRTSRAVWKA
                     IRHMIVRGAPAIGIAAGYGLYLGVRGFRGGRYEFMQELSRQGRYLDSSRPTARNLAYA
                     LERILNRVCLSRQDHIVDLKRLVLEEARQIHLDDIVLCRKIGQYGSRLFKRGDTILTH
                     CNAGGLATSGYGTALAVFYALKEKKIPFSVFVDETRPLLQGARLTAWELSRSKIPATL
                     ICDNMAASLMRARKIQGVVVGADRIAMNGDTANKIGTYGVAVLAHVHRIPFYIAAPST
                     TFDLKAKTGKDIPIEERPHDEVRKLHGKQLAPKGIKVFNPSFDVTPAKYISGIITEAG
                     VLRPPFARSIGKVKRLLEHA"
     gene            complement(4968..>5941)
                     /locus_tag="PLY30_00380"
     CDS             complement(4968..>5941)
                     /locus_tag="PLY30_00380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPW64585.1"
                     /translation="AQSSLSGLEQFRAAERKFSDETLSEPAFGNFARKEPLLAAGFGA
                     YTALLGLALTVGFVINFLAVFRPSFREKILPRLPPPSETRVWPFSILFKTVVLFVVWS
                     ILISFLMGVVSAIFPVEGADNAYMVLHTFILNVLCVMFMVRFLKQQGSSWRDLGFNVP
                     QGKPFREMMIGWTGYLGVLPIFALVLAVLLFLANLVRYEPPPHPLVNVFIEEEKRLPF
                     LVAFSAFLGAVIGPVFEEVFFRGFCYPILKGKVGKVWAMVISAAFFASIHHTGFVFWP
                     IFVLGLALAYLYESRRSLLAPITLHVTHNTLFISYFFLVKQMISSSA"
BASE COUNT         1340 a         1835 c         1535 g         1231 t
ORIGIN      
        1 ttcgttgaaa atcttgtgcc ggagccagga cgcgaatcgg catctcagac cgctcccgca
       61 cggtaccagc gccgtcatgt gctgggcgat gctgacggca ggctcccgga ggccaaaatc
      121 tctgcagacc tgatggatca tgcgaaaaac cgcaacatgc gcgcgaacca tgttccggct
      181 gacccgccac atcctccaaa gcgacctggc ttgaggaggc caaacgccaa agagaaacga
      241 gtgagagaca tagatcgtcg gctcgttgat cgtaatccag taccggacat gcttgccgag
      301 aatcctcacc acagcgtcac agtaccggag aaatctcccg ggggcgcgtc gatcttccca
      361 gccgccgtcc cgcgcgaacc agagagggtt tgtgaaatga tgcaaggtca cgaccggctc
      421 aatgccccgg tctttgaggg caagcacgac ctccgcgtaa tgtttcaggg catcgtcact
      481 gaatcttccc tcttgaggct cgacgcgggc ccactcaacc gaaaggcgat gggcgttgtg
      541 gtggaggctt ctcgcaagat caaaatcccg ctcatacaac tcataatgac gacacgcatc
      601 gccggaccgc tccctccccc gtgtctgttc ccactcccac cagtcggaac cgatgttgcc
      661 cccctccacc tgatgagcgg ccgtcgccgc gccccacagg aatccgtccg gaaatttttt
      721 catgccggaa gtatagcaca ctcggcatcc ttgttttata ccctcgaaac agatagatta
      781 cacgccatgc gctccctcat cggacagatc aaaaaaactt atcgcatggc cggccggcac
      841 gaacgccgga ccgtgcttga gaatttcgcc tcgctttcga tccttcaagc gatcacgtac
      901 atccttccgg tcatcatcat cccttacctg ttccgggtca tcggtcctga caagttcggc
      961 ctgatcgctt tcgcgcagtc cttcatccag tatttcatga tcctgaccga ctacggattc
     1021 ggcatctcgg ccaccaggga gatctctctc taccaggatc gccgcgccag gatcagcgaa
     1081 atcttttccg cggttataac ggccaagatc gtcctggccg cgttgagcct gatgatcctg
     1141 ggcgccattg tctacctggt cccgcgcttc cgcgcggact ggatggtcta ccttttcagt
     1201 ttcggagcgg tcatcggaaa cacaattttc ccggtgtggc tgttccaggg aaccgagaaa
     1261 atgaagcaca tcgcggatct caacatcctc ggcggagtcc tcttcgcgct tttcgtgttc
     1321 atgtttgtca agagcccgtc cgactacctc atagtaccgc tcgtctattc cgttgtctct
     1381 atcatgacgg gcctttgggc cctcttcatc ggctttacga aactcggaat ctccttccgg
     1441 ttcccgggct acacctccat gcagcagcag atgaaggcgg gttgggacat tttcatatcc
     1501 aacgtcgcga tcaacgccta tacgaccacg cgcgtgttca cggtcgggct tctcacgaac
     1561 aacaccctca cgggatatta ttcgatcgcg gaaaggatcg ccgggttctg ccagcagttc
     1621 ccgctctcat cgttttcaca ggcactcttc ccgaggctca gcaagatctt ccagaaaaac
     1681 cagcggcgcg cctatgaatt catgagaaaa atccagcaga tcacggtctc gatctccctg
     1741 atctgtcttc cgctgatctt cctcttcgcc gggccgatcg tccgcatcat ctgcggcggc
     1801 gattcacccc aggccgtcct gaccctccgg atccttcttt gcgcggtctt tatcgtcggc
     1861 gcaaacgcct tccggatcca gttcctgctg atctgcggca agacctccat ttactccaga
     1921 atccacatcg tcatggcgat ggtgggtctc ccgttgatct ttctcttgat ctattctttt
     1981 tcctatgccg gagccgccat cgcaaccgtc atcatcgaag tcgggatctt cacgatcacg
     2041 tacttcacag tcaaacagtt aagactaaaa tgaacgcccg cccggaagga cacaatggga
     2101 aaaacgatat ttcccgccat gggaatatcg catgaaaaac atccccttct tttcaggagc
     2161 cgacgcatct ttgctgcccc tgtttggccg gtggcaggaa cttccggaaa cgcaggtgta
     2221 acgatcgcta aaaataaaag gcacagcaac gttcctgact cgccgctgcg ccttggctat
     2281 aaccgttctg cgatgcttac aacatcgaat ttaaagattc atcctcaggt tcttcgtcag
     2341 gccccggctc gggcaattca attttggggg tcgtttggtt cgtcggtcgg caactccacc
     2401 ggcatcagcg caggcggttc cggatcatgt catcctccgc cccttcccgg accttttcac
     2461 atcggcgaca gcctttgggc aaacaatatc gatcttccgc tcgcattcgc caaggatcgc
     2521 caaccggacg tggtaagcgt gcggcggaat cagatcaccc gccttctcag cccattcgat
     2581 acagcaaatc gctcccggat ccgccgcaaa atcccccact ccgatattgg caagttcgtc
     2641 gaccgaatcg atccggtaac agtcaaaatg atacaaggga atctttgtcc ggtacacatg
     2701 catgaggaca aaggtcgggc tctttacctg ttcggaacgt ggaagcccaa gacctgccgc
     2761 gactcccttg ataaacgttg ttttcccgct tccgagatcc ccttcgaggc aaagaacact
     2821 tcccggtttc agggtcaacg caaacctttc cgcgaacgcg atcgtttcat cggaagacgt
     2881 tgtaatggtc gacaatttct ccatgatcta aaaatgcctg aatcctttcc gcgacaaccc
     2941 ccgccgcatc cggacccgct tgtcatttga gatccagcga ataccgggct ttcccttgac
     3001 gatccggccg atccagttca gcgaaacgcc ccggcactcc tttttccagc gtcgttccag
     3061 aacggatttc gcgggtccgg gcacggtgaa caaaagctcg aaatcctcgc catcggtcaa
     3121 ggccctctcc agagcggcca ggctgttctt gcgaccgaga tggatcgcgt cggacgaaac
     3181 gggaattcgc cgcggatcaa gctcggcggc cacacaagac gcgttcagaa tatgatgcag
     3241 gtcctggcaa aggccatccg agatatcaat catcgaatgg acaagaccgc ttcgggcgag
     3301 cgcaaggcct tcgcggacac gcggggtgaa atcaaaatga tgacgcagga ttgaacctcc
     3361 caggcgtccc gtcaccgcga tccagtcgcc cgctttcgcg ccggagcgga gaatcggttt
     3421 tcgggctgtc ccaaagatcg taacgcttgc ggaaaactct tttgcggccg agaaatcccc
     3481 gcccacgcaa tcgactttgt accggcgagc cagtctcaga agtccgtcat agaaacggtc
     3541 aagccaccgg gcatcaacgt aagcgggttt gccgatacta atgacgaacg cgagcggttt
     3601 ggcgcccatt gccgcgagat cgctcaagtt cacggccaat gccttgcgcc cgatctgttc
     3661 cggctcggac cggttcagga caaaatcaac cttttcgacg atcatgtccg ttgaaaccac
     3721 aagtcgcccg ggaccggaaa accttaaaac cgccgcatca tctccgatgc caaggacagc
     3781 gttcttcgcg ggcgggatct tttttttcaa tctttcgata aaagtgaact catccatcac
     3841 ggcctgctcc ccagtcccct gcgtaacagg gtcttgtgat catgcgtgct caagaagccg
     3901 tttcaccttc ccgatcgacc tggcaaaagg aggccggagg acccccgcct cggtaatgat
     3961 gccggagatg tatttcgcgg gtgtcacgtc gaacgacgga ttaaagacct ttatcccctt
     4021 cggcgcgagc tgcttgccgt ggagcttccg gacctcgtca tgcggacgct cctcgatcgg
     4081 gatatccttc ccggtcttcg ccttgaggtc gaatgtcgtg ctcggggcgg caatatagaa
     4141 cgggattcgg tgaacatggg cgaggaccgc cacgccgtag gtcccgatct tgttggccgt
     4201 gtcaccgttc atcgcgatcc ggtccgcgcc cacgaccacg ccctggatct tgcgagcccg
     4261 catcagactc gcggccatat tatcgcaaat aagcgtcgcc ggaatcttgc tcctgctcag
     4321 ctcccacgcc gtaagccgcg cgccctgaag gagcgggcgg gtttcatcaa cgaagaccga
     4381 gaacggaatc ttcttttctt tcagggcata gaaaaccgcg agcgccgtcc cgtagccaga
     4441 ggttgcgagc ccccccgcgt tgcaatgcgt gaggatcgtg tcaccccgtt tgaagagcct
     4501 tgaaccatac tgaccgatct tccggcaaag cacgatatca tccagatgga tctgccgtgc
     4561 ctcttcgagc acaagacgct tgaggtccac aatgtgatcc tgacgactca ggcatacccg
     4621 gttcaagata cgctcgagcg cataggcaag atttcgagcg gtcggacgcg aagaatcaag
     4681 gtaccggccc tgacggctca gctcttgcat gaattcatac cgtccgccgc gaaatccgcg
     4741 gacgccgagg tagagaccgt atcccgccgc gatcccgatc gccggggcgc cccgcacgat
     4801 catgtggcgg atcgccttcc aaacggcccg ggaggtccgg caagcgaccc aaacctcccg
     4861 gttcgggagt tttctctggt caagcagata aagcgtttcc ttgcggaatt caaccggaga
     4921 gaacgggact ttgggatttt tcatgggcac attctacctc gtttcgatca ggcgctcgag
     4981 ctgatcattt gtttgaccag gaaaaaatag ctgataaaaa gcgtattgtg cgtgacgtgc
     5041 agggtaatgg gagcgaggag cgacctccgg gattcgtaga gatacgcgag ggcaaggccg
     5101 agcacaaaga tcggccagaa cacgaaaccc gtgtgatgga tcgaggcaaa aaacgcggcg
     5161 ctgatgacca tcgcccaaac cttcccgacc ttccctttga ggatcgggta acagaacccc
     5221 cgaaaaaaga cctcctcgaa gacaggaccg atcacggcgc caaggaatgc ggagaacgcc
     5281 acgagaaacg gcagacgttt ctcctcttcg atgaaaacgt tcacgagcgg atgcggcggc
     5341 ggctcatagc gcaccaggtt cgccagaaaa agcaacactg cgagcaccag ggcgaagatc
     5401 ggcagcaccc cgagatatcc cgtccacccg atcatcatct cccggaacgg cttcccctgg
     5461 ggaacgttga atcccaggtc ccgccaggaa cttccctgct gcttgagaaa ccggaccatg
     5521 aacatcacgc aaaggacatt caggatgaac gtgtgaagaa ccatgtaggc attgtcggcg
     5581 ccctcgaccg gaaaaatggc gctgaccact cccatcaaaa agctgatcag gatactccac
     5641 accacaaaaa gaaccacggt cttgaagagg atcgagaaag gccagacgcg ggtctcggaa
     5701 gggggaggaa gccgcggcag tatcttttcc ctgaacgagg gacggaacac ggccaaaaaa
     5761 ttaatgacga accccacagt gagcgccaga ccgagcaatg ccgtgtaagc gccgaacccg
     5821 gccgcgagaa gcggctcctt cctggcgaaa tttccgaatg ccggttcgga caatgtctca
     5881 tcgctgaatt tccgttcggc cgccctgaac tgttcgagac ccgacaaaga tgattgcgcc
     5941 c
//
LOCUS       DAOOBX010000012         5894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_15684_length_5894_cov_3.728036, whole genome shotgun sequence.
ACCESSION   DAOOBX010000012 DAOOBX010000000
VERSION     DAOOBX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15684_length_5894_cov_3.728036"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PLY30_00385"
     CDS             <1..892
                     /locus_tag="PLY30_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HPW64586.1"
                     /translation="GGVPGFETTRFSITSHRGCSAECSFCSLHAHQGRIIQSRSKGSI
                     LAEARKLAARPDFKGTITDIGGPTANLYGADCEFWTRQGACRAKQCMMPGKCGNLRLG
                     YEKAVDLWRDVMKVPGVKHLFIQSGLRYDLLIDQASDDYLRALCAEHVSGRLKVAPEH
                     CDPRVLALMNKPELGKYAKFEERFVRFSREAGKKQYLVNYWISAHPGAGISEAEDLAE
                     YFHERNIRPEQIQDFLPLPMTVSGAMYWTGRHPFTGEPVRVARTHKEREAQRLKVQPQ
                     SPSRSLLKGGKERVSGKRKS"
     gene            889..2088
                     /locus_tag="PLY30_00390"
     CDS             889..2088
                     /locus_tag="PLY30_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HPW64587.1"
                     /translation="MNYYDMLVIGGGPAGMMAAIFARRNGKSPVILEKMPKAGKKLLV
                     TGAGRCNLLNERLDASFYKPEARPLVEEVFRKSGKEKILGFFEDLGLRVFSDDTGRIF
                     PVTQQASSVLRVLELELARLKVPVECGCGVKELRAVAGGFEARCASGAKYTGATAVIC
                     GGGKSYPALGADGDAYGLAKKTGHSITEPVPSTVPLVVKDPWCHHLQGQKLRAVVRVL
                     DGDRILKEAAGELLFTQYGLSGTAVLDASDPVSIAINRSHQKEMTIEADLLPFLGEEE
                     LEKELGRRIGRGFPREALLEGLLPSRVAAAVLRSLEPPDARGLKELVRIVKHRKFKVQ
                     GTRGWNEAEFTAGGVPAAELDGNLGSKIVKGLSFAGEIVDVQAARGGYQLAWAWASGA
                     VAGSVRL"
     gene            2085..2561
                     /locus_tag="PLY30_00395"
     CDS             2085..2561
                     /locus_tag="PLY30_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HPW64588.1"
                     /translation="MKKRHAWSLGICAALALFLFAVFLSNPLKNGIVSFMKLEEGVVG
                     GGLVSEAAFRAAAAAGYRTVIDIRTREEGIEKEKQFVESLGLRYVNIPFHRGKAGSEE
                     ALRLDEVLSEKGAKPAILHCRSGNRVKMFWAMYQKMKRDGEPDRAPETASPERRSA"
     gene            2815..3663
                     /locus_tag="PLY30_00400"
     CDS             2815..3663
                     /locus_tag="PLY30_00400"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW64589.1"
                     /translation="MKIAVLGLGIIGSIWARHLAADGHEMKCWNRTPRKDFPGVSATA
                     AEAVESAEAILLVVADPPAVRTVIDQIAPALERGQMLIQASTISAEWTLKFAREIRKT
                     GVLFIEAPFTGSKPAAEQRKTVFYLGGDPGVAEKAKPVLGPLSSVLFYVGELGSASTL
                     KLAMNLNIAQVGQALCESLAMCRAAHIPDDVYFKALSLNASRSGLSDFKGPKLKEQDY
                     SPQFSLKHMDKDLRLALETAAALGLTLEQTARLKKLYDLGMARGWQEEDFIGLIRLLE
                     GRGGRE"
     gene            3660..4445
                     /locus_tag="PLY30_00405"
     CDS             3660..4445
                     /locus_tag="PLY30_00405"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPW64590.1"
                     /translation="MMESRKLKPEEDLYLETGYRILHSENAFLVIEKPAPLPVHPVGR
                     FRKRNLLSFLKKDFGSEGASLRIVNRLDSETSGIVLVSRLPEMAGPLGLLFERRRIRK
                     EYHAVVLGAPAKDHGTIDVPLGLRPGGVHNIRVPDPDGQEARTDYEVLSRAGDYSLLK
                     LVPHTGRTHQIRVHLAHLGHPVAGDKIYIDPAVFERYIHEGWQEDMRSVVKAERLLLH
                     ASGLEFIHPVTEEKLKFRSALPECFSEFLVAGDRAEEKKERER"
     gene            4442..4846
                     /locus_tag="PLY30_00410"
     CDS             4442..4846
                     /locus_tag="PLY30_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPW64591.1"
                     /translation="MKRKEVPEKSRKEEMTSAEFEALVRETVKGLPAEFARRLENVAV
                     VIRAEPSARQKKESGGEEELLGLYEGVPYGERTHHDGPVFPDRITIFRGPILRSCQDR
                     REIRRAVRDTVLHEFAHYFGLSDEHMIAEDIY"
     gene            complement(4889..>5894)
                     /locus_tag="PLY30_00415"
     CDS             complement(4889..>5894)
                     /locus_tag="PLY30_00415"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64592.1"
                     /translation="RILLPSPERVEPPCPYVRACGGCQYQHLRYEEELRWKELQVRES
                     FAKELRADAPFIQPIRRGPKEYGYRQSITLHRTVKTNQKPHPLAFIGRDNHSFVFIEN
                     CMLADENLKSVFAAPHALGKYEIKRVFKIGQDKGIVSSEEDRLYRVPLGGEVFWAHSR
                     GFFQNNLDVTELVAEEVAEIVRETAPSRFIDLYSGVGTFTVLSAPKIPELCCFEQSPY
                     AIDCLRRNLNERGLPGAIFRGKVEQMFPNFWLGNRKEDSFILLDPPRLGITPQLAEFF
                     SKADMITNLVYLACDLQILLRDLRIILSKGNYRIRTVVPFDMFPRTKHLEVLAWLERA
                     "
BASE COUNT         1205 a         1658 c         1927 g         1104 t
ORIGIN      
        1 cggtggggtc ccgggattcg agacgacccg cttttcgatc acgtctcacc gcggctgctc
       61 ggccgaatgc tccttttgtt cgctccacgc gcatcaggga cggatcatcc agagccgcag
      121 caaagggtcg atcctcgcgg aggcgcggaa gctcgccgcc cgtccggact tcaagggaac
      181 gatcaccgac atcggaggcc cgaccgcgaa cctttacggc gccgactgcg aattctggac
      241 gcggcaggga gcgtgccgcg cgaaacagtg catgatgccc ggcaagtgcg ggaacctgag
      301 gctcggctac gaaaaggccg tggacctctg gcgcgatgtc atgaaagtgc cgggcgtaaa
      361 gcatcttttc atccagagcg gcctgcgcta cgatctcctg atcgatcagg cttcggacga
      421 ttacctgcgc gcgctttgcg cggaacacgt gagcggccgg ctcaaggtgg cgccggagca
      481 ctgtgatccg cgggtcctcg cgctcatgaa caagccggag ctcgggaagt acgcgaaatt
      541 cgaggaacga tttgtccggt tcagccgcga ggccgggaaa aaacagtacc tcgtcaatta
      601 ctggatcagc gcacatcccg gcgcgggcat cagcgaggcg gaggatctcg cggaatattt
      661 ccacgaacgg aacatccggc cggagcagat ccaggatttt ctgccgctcc cgatgactgt
      721 ttccggcgcg atgtactgga cgggcaggca tccgttcacg ggcgaacccg tgcgcgtcgc
      781 gcggacccac aaggaacgcg aggcgcagcg gctgaaggtg caaccccaat ccccttcccg
      841 ctccctttta aaagggggga aggagagggt ttcaggaaag cgaaaatcat gaactattac
      901 gatatgctgg tgatcggcgg cggtcccgcg ggcatgatgg cggcgatctt cgcgagacgg
      961 aacgggaaat cccccgtgat cctcgagaag atgccgaagg ccggcaaaaa actcctcgtc
     1021 acgggggccg gccgctgcaa cctcctcaat gaaagactcg acgcctcttt ctacaagccg
     1081 gaggcccggc cgcttgtcga agaggtcttc cggaaatccg gaaaggagaa gattctcggg
     1141 tttttcgaag acctcggact gcgcgttttt tccgatgaca ccggacggat ctttcccgtg
     1201 acccagcagg cctcctcggt cctcagggtc ctcgaactcg aacttgcgcg gctcaaggtc
     1261 cccgtcgagt gcggctgcgg cgtgaaagaa cttcgcgcgg tagcgggtgg tttcgaggca
     1321 cgctgcgcgt cgggcgcgaa gtataccggt gcgacggccg tgatctgcgg cggaggaaag
     1381 tcctacccgg cgctcggcgc ggatggtgac gcatacggtc tcgcgaaaaa aacgggacat
     1441 tcgatcacgg aaccggtgcc gagcacagtg ccgctcgtcg tgaaagatcc atggtgccat
     1501 cacctgcagg gtcagaagct tcgcgcggtc gtaagagttc tcgatggcga caggatcctg
     1561 aaagaggccg cgggcgaact gctttttacg caatacgggc tttcggggac ggcggtgctc
     1621 gacgcgagcg atcccgtttc gatcgcgata aaccgcagcc atcaaaaaga aatgacgatc
     1681 gaggcggatc tcctgccttt cctcggggaa gaggagctcg aaaaagagct cggccgccgg
     1741 atcggccggg gattcccccg ggaagccctt ttggaaggtc tcttgccctc cagggtggcc
     1801 gcggcggtct tgcgttctct cgagcccccg gatgccaggg gtttgaagga acttgtccgg
     1861 atcgttaaac accggaaatt caaagttcag ggcacgcgcg gctggaacga ggccgaattc
     1921 acggcgggcg gtgttcccgc cgcggaactg gacgggaacc tcgggtcgaa gatcgtaaag
     1981 ggactttctt tcgcgggcga gatcgtggac gttcaggccg cgcgcggagg ctatcagctt
     2041 gcgtgggcgt gggcgagcgg cgctgtcgca gggtcggtac ggctatgaaa aaacgccatg
     2101 cctggtccct cgggatttgc gccgccttgg cgctattcct gttcgccgtg tttttgagca
     2161 atccgttgaa aaacggcatt gtcagcttta tgaagctgga agaaggagtt gtcgggggag
     2221 gtcttgtcag cgaagcggcg tttcgggcgg ccgcggcggc tggctaccgg accgtgatcg
     2281 atatcaggac ccgcgaagag ggtatcgaaa aagaaaaaca gtttgtggaa tctctcggcc
     2341 ttcggtacgt caacatcccg ttccatcgcg gaaaagccgg ttccgaggaa gcgctccggc
     2401 ttgacgaagt tctttcggag aaaggggcca agcccgcgat tctgcattgc cggtcgggaa
     2461 accgggtcaa gatgttctgg gccatgtatc aaaagatgaa gcgtgacggg gaaccggaca
     2521 gggctccgga gaccgcgtcc cccgagagga ggagcgcgtg atggtcgatt acgtgacatg
     2581 gacgctggcg aacgtagccg ccggattttt tgtgctgtca gggttccttg cgagcaaaga
     2641 gggaggcccc ggggcaggct tgggcgtccg tgttcgctgc gacagggctg acgctcgggg
     2701 cggcatggcc gctcgcgcga gggctcgacc tgagatctcc tgtatggcct ggcggaatca
     2761 ccctgaaaat gttcataaag cgatcgacat gacggagaag gcgggagcgc ccggatgaag
     2821 atagcggtat tagggctcgg gatcatcggc agcatatggg ccaggcatct ggccgcggac
     2881 gggcatgaaa tgaaatgctg gaaccggacg ccccggaaag atttccccgg ggtcagcgcg
     2941 accgccgctg aagcggtgga aagcgctgag gccatcctgc tggttgtcgc ggacccgccg
     3001 gctgtccgga cagtgatcga ccagatcgcg ccggcgctgg aacggggaca gatgctgatc
     3061 caggcgagca cgatctcggc ggaatggacg ctgaagttcg cgcgggagat tcggaagaca
     3121 ggggttcttt tcattgaagc gccgttcacg ggaagcaagc ccgcggccga acagcgaaag
     3181 actgtttttt atctcggcgg tgatcctggt gttgcggaaa aagcgaagcc ggtcctcgga
     3241 cctctttcga gcgtgctttt ttatgtcggg gaactgggct ctgcttccac gctgaagctc
     3301 gcgatgaacc tgaacatcgc ccaggtcggc caggccctct gcgaaagtct cgcgatgtgc
     3361 cgcgcggcac atattcccga cgacgtttat ttcaaggcgc tttcactgaa cgcctcacgg
     3421 tccggcctct ccgatttcaa ggggccgaag ctgaaagaac aagattacag ccctcagttc
     3481 tcgctcaagc acatggacaa ggatctccgc ctcgccctcg aaacagcggc ggctctcggc
     3541 ctgacgctcg aacagaccgc gcgtctcaag aagctctacg atctcggaat ggcacgcgga
     3601 tggcaggaag aggattttat cgggctcatc cggcttttgg aagggcgcgg cgggcgggaa
     3661 tgatggaaag ccgcaagttg aagcccgaag aagatctcta tctggagacg ggctacagga
     3721 tcctgcatag cgaaaacgcg tttctcgtga tcgagaagcc cgcgccgctt cccgtgcatc
     3781 cggtcggacg gttcaggaaa agaaaccttc tgagttttct caaaaaagat ttcggatccg
     3841 aaggggccag cctcaggatc gtcaaccggc tcgattccga aacgagcggg atcgtgctgg
     3901 tttcgcgctt gccggagatg gccgggcccc tcggactttt gtttgagaga cgccggattc
     3961 gcaaggaata tcacgcggtc gtgcttggag cgccggcgaa ggatcacggt acgattgacg
     4021 tcccgctcgg tctcaggccc ggcggtgttc acaatatccg cgtccccgat ccggacggcc
     4081 aggaagcccg gacggattat gaagtcctga gccgcgccgg ggattattcg ctcctcaagc
     4141 tggtcccgca tacgggacgc acgcatcaga tccgcgttca cctcgcgcac ctcggccatc
     4201 cggtcgcggg ggacaagatc tatatcgacc ccgccgtgtt cgagcgctac atccacgaag
     4261 gctggcagga agacatgcgt tccgtcgtaa aagccgagcg cctgcttctg cacgcgtcag
     4321 gtctcgaatt catccatccc gtgacggaag agaaactgaa attcagatcc gcgctcccgg
     4381 aatgtttcag cgagttcctg gtcgcgggtg atcgcgcaga ggaaaagaag gagcgggaaa
     4441 gatgaaaaga aaagaggttc cggaaaagag ccgcaaggaa gagatgacgt ccgccgagtt
     4501 cgaggcgctc gtccgggaaa cggtgaaggg tctccccgcg gaattcgcgc gaaggcttga
     4561 gaatgtcgcg gtcgtgattc gcgctgaacc gtcggcccgg cagaagaagg agagcggcgg
     4621 agaagaagag ctcctgggcc tctacgaagg tgtaccgtat ggcgaaagga cccaccatga
     4681 cggtcccgtt ttcccggacc ggatcacgat cttccggggc ccgatcctgc gttcctgcca
     4741 agatcgccgt gagatccgtc gagcggtacg ggacacggtc ctgcatgagt tcgcgcacta
     4801 cttcggcctc tcggacgaac acatgattgc ggaagacatc tactgagcgg ccgggctgtc
     4861 gttttttgtc aaaaacggct gtccccggtc acgcgcgctc gagccaggcg agaacctcga
     4921 ggtgtttcgt gcgcgggaac atgtcgaagg ggactacggt ccggatccgg taattccctt
     4981 tcgagagaat gatccggagg tccctcaaga ggatctggag gtcgcaggcg aggtagacga
     5041 ggttcgtgat catgtccgcc ttcgagaaga actcggcgag ctgcggcgtg atcccgagac
     5101 gcggcgggtc gagcagaatg aaggagtctt ctttccggtt ccccagccag aagttcggga
     5161 acatttgttc gacctttccg cggaagatcg cgcccggaag gccacgttcg ttcagattgc
     5221 gcctgaggca gtcgatggcg tacggacttt gctcaaagca gcagagctcg gggatcttcg
     5281 gggccgagag gaccgtgaag gtgccgacgc cggaatagag atcgatgaaa cgcgaggggg
     5341 ctgtctcgcg gacgatttcc gcgacctctt ccgcgaccag ttccgtcaca tcgaggttgt
     5401 tctgaaagaa tccgcgcgaa tgagcccaga acacttcacc cccgagcgga acgcggtaaa
     5461 gcctgtcctc ctcgcttgag acgatcccct tgtcctggcc gatcttgaag acgcgtttga
     5521 tctcgtattt accgagcgcg tgcggcgccg cgaaaacgct tttaaggttc tcgtccgcaa
     5581 gcatacagtt ctcgatgaaa acgaatgaat ggttgtcgcg gccgatgaac gcgagtgggt
     5641 ggggtttctg gttggtcttg accgtgcggt gcagcgtgat gctctgccgg tatccgtatt
     5701 ccttcggccc ccgacggatc ggctggatga agggcgcgtc cgcgcgcagt tccttcgcga
     5761 aactttcacg cacctgcagc tccttccagc ggagctcttc ctcgtagcgg agatgctggt
     5821 attggcatcc gccgcaggcc cgaacgtagg gacagggtgg ttcgacgcgt tcgggggaag
     5881 ggagcaggat cctg
//
LOCUS       DAOOBX010000013         5852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_15807_length_5852_cov_3.577540, whole genome shotgun sequence.
ACCESSION   DAOOBX010000013 DAOOBX010000000
VERSION     DAOOBX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15807_length_5852_cov_3.577540"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLY30_00420"
     CDS             complement(<1..455)
                     /locus_tag="PLY30_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW64593.1"
                     /translation="MAELLKLKSPADFIGKQGHGYISFGSGQPDLPPPKEAFEGINLT
                     RDLRYGLIQGEFALRDALTHEYPGSTAENFVITNGASEALDLIFRIWGKGEKVLLPRP
                     YYYSYPPIIEMAGMVPVYTDMVEGRIDLEDFRKKVTGCRAVLINSPSNPT"
     gene            complement(569..907)
                     /locus_tag="PLY30_00425"
     CDS             complement(569..907)
                     /locus_tag="PLY30_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64594.1"
                     /translation="MREVLVKGLAGNGDEGTIKCWYFEEGDRVTKGDDLLELVTADES
                     VVIIPVPVNGILAEVYFGEGETVQRDEVICTIDDESKELRDEDEDDPDDEEDETDGRD
                     GDDEESEEDY"
     gene            complement(900..1829)
                     /gene="lipA"
                     /locus_tag="PLY30_00430"
     CDS             complement(900..1829)
                     /gene="lipA"
                     /locus_tag="PLY30_00430"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPW64595.1"
                     /translation="MAQYYRRFPDWLRRSLPESSTVRTHAILKKYQLNTVCRSAMCPN
                     SHECFAEDTATFMILGKVCTRHCGFCAIASNSQGEVLRADEPERVAFAAKELGLKYVV
                     ITSVTRDDLEDEGAAHYARTIRAVQNLIPGVKVEILTPDFHAREDLIARVLEAEPDVF
                     NHNLETVERLQRVVRPQADYLRSLNVLKTAKRIEPKCVTKSGLILGLGETEAEVLETG
                     RQLLASGVSILTLGQYLRPTSNHIEVKEFVPPRKFEELSKRLEAMGFSRVIAGAYVRS
                     SYHAAEAFAGGGRVHGKYADGIVTEEKGVDRNA"
     gene            complement(1951..2619)
                     /locus_tag="PLY30_00435"
     CDS             complement(1951..2619)
                     /locus_tag="PLY30_00435"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HPW64596.1"
                     /translation="MRPVTSARMREIDRISSEKFGIPGRTLMENAGRALARIIYLDPT
                     CRKVLIICGKGNNAGDGLAAARYLLERDIEVSILLIDPPSVFSPEALLQYNLLKGSRA
                     AFYRLRDHDAESIMAAILPESQVVLDAIFGFGFRGRISGLHERVVRAINASGKTVYSA
                     DLPSGLNADDGTVAGTSVKATVTVTFGLPKAGLFLAEGPALAGRVEVVDIGHPPELLA
                     PYRD"
     gene            complement(2641..4422)
                     /locus_tag="PLY30_00440"
     CDS             complement(2641..4422)
                     /locus_tag="PLY30_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64597.1"
                     /translation="MNFDHMKGVSSNENWCVIIQSFPSKLRKELVKQVADIFGLDKKD
                     AEQAFRNTPLILLDGLSFSTAARIKNFFAKVGANVETTSHEMIKKNCYQILWSEMPDL
                     SFFLKDEAPAAGARPAKPAPVLPATPEKATKPEESSPAEKPPVPAKHAFSFLRQQTPE
                     PAPATAETPARSAPKPAAAPEPAPEPKADTQEQAVTPEPASLWEKRAKELSSRLAKIQ
                     DEKQQQQQQQQIQASREQDRLKEDMQAKLAAEKQQAEQPAQEREQAQSSPGATEESAV
                     REAERAKEQNEALLQTIKDLETRLGQKDSDLRTAQQRLGEFTQKEVGATARADQLQKT
                     LDEKNAAITEGENLRAALQQEISQLTGKVGDLDGIAKHRQAELESMQAEVEKLCAKHD
                     EERRTLQSQTGDLNKKIEELEAVIREKQNSSDEIQRTIDELRSREAELQQQNGSLVHA
                     LNRADEMLRARESEMKERDRMLDEFEKKIGELIQQNQEVEAMRRENARFDAERSALLQ
                     EFESRFAEQEARILKVEEESRRARSRVERKLTAANRELGEWARGVEALKAGLQKLALF
                     LVTDAPEVEKKSPLRASAPSKLQFPRT"
     gene            4527..5501
                     /gene="dusB"
                     /locus_tag="PLY30_00445"
     CDS             4527..5501
                     /gene="dusB"
                     /locus_tag="PLY30_00445"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPW64598.1"
                     /translation="MLTIKDRTFKTAVIQTPLAGCTDLAFRLIARDHGMELAFTEMIS
                     SEALVRAHKKTYTLLKRAREDSPTGCQIVGPDPDSMGRSAAILEPLGFDILDINCGCP
                     VRKITGQEAGAALLKDPKRAGKIFRSVIRSVKAVPVTVKMRTGYTDPSGKEAVRLARI
                     AEDCGLSAVTVHGRTKAQGYSGKADWRIIESVKKVVTIPVIGNGDVFSAADALRMRSE
                     TGCDGIAIGRGSLGNPWIFGQIRAAFGHNKRSVPSFREVKQTALRHMDLTALWDGEKT
                     AHLKSRHIACWYFKGCPGSSRLRERINRSRSFEDIVSIVKAYEPHRPV"
     gene            complement(5520..>5852)
                     /locus_tag="PLY30_00450"
     CDS             complement(5520..>5852)
                     /locus_tag="PLY30_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HPW64599.1"
                     /translation="GINEHMPEVKRRVPFSNMIYIGDGITDVPSMTVMNKSGGHAIAV
                     YDPSGEVPREVKEMIAEKRAEHFAPADFRPNALLIKILHRTLKQIIHSISFRLSSKRS
                     TDWVRRHW"
BASE COUNT         1141 a         1804 c         1590 g         1317 t
ORIGIN      
        1 gtggggttcg acggcgaatt gatgagcacc gccctgcatc ccgtcacctt cttcctgaaa
       61 tcctcaagat caatgcgccc ctccaccata tccgtgtaaa caggaaccat ccccgccatt
      121 tcaataatcg gcggatacga gtagtaatac ggcctgggca aaaggacctt ctcacccttc
      181 ccccagatgc ggaagataag gtccagtgct tctgacgcgc cgttcgtgat cacaaaattc
      241 tcggccgtcg atccgggata ctcgtgggtc agggcatccc gaagcgcaaa ctcaccctgg
      301 atcagtccgt agcgcaaatc ccgcgtcaaa ttgatacctt cgaacgcttc cttgggcggc
      361 ggaaggtccg gctggcccga accaaaactg atgtatccgt gcccctgctt gccgataaaa
      421 tcggccgggc ttttgagctt cagcaattca gccacgacaa caccactcgc attccgtcaa
      481 aggggaatcc gccgccggca tgatccgttc ccttccgtct cgcaacatca tctcgcgtct
      541 cccggttcaa ttcataaaac aagttcgttc agtaatcctc ttcggactct tcgtcatcgc
      601 cgtcgcgccc atccgtttca tcctcttcat cgtccggatc atcctcgtcc tcatcccgca
      661 gttctttcga ttcatcgtcg atcgtacaga taacctcatc gcgctgcacc gtttcaccct
      721 cgccaaaata aacctctgca agaatcccgt tcacggggac cggaatgatc acgaccgatt
      781 catcggctgt gacaagttcc agaaggtcgt cgcctttggt caccctgtcc ccttcttcaa
      841 aataccagca tttgatcgtt ccctcatcac cgttgccggc gagccctttc accagtacct
      901 cacgcattcc gatcgactcc tttttcttcg gtcacaatcc catccgcgta ttttccatgg
      961 acgcgcccgc cacccgcgaa agcttcggcc gcgtgatatg aacttcgaac gtaggccccc
     1021 gcgatcacgc gggagaaccc catcgcctcg aggcgcttcg acaattcttc aaacttgcgc
     1081 ggaggaacga actccttgac ctcgatatga ttcgaagtcg gcctgagata ttgtcccagc
     1141 gtcagaatcg ataccccgct cgccagcaat tgccgtcccg tctccagaac ctccgcttcc
     1201 gtttccccca atccgaggat gaggccgctt tttgtcacgc atttcggctc gatccgtttg
     1261 gcggttttca gaacattcag cgagcgcaga taatccgcct gtggccgcac cacccgctgc
     1321 agacgctcta ccgtctcgag gttatgatta aacacatccg gctccgcctc caggacccgg
     1381 gcaatcagat cttcccgcgc gtgaaaatcg ggcgtcagga tctcaacctt gacgcccggg
     1441 atcagatttt ggacggccct gatggtccgc gcgtaatgag cggcgccttc atcctcaagg
     1501 tcatcccgcg tcacggaagt aatgacgaca tacttcaagc cgagctcttt ggccgcgaac
     1561 gccacacgtt ccggctcgtc ggcgcgcaat acctctccct ggctattact cgcgatggcg
     1621 caaaacccgc aatgccgcgt acagaccttg ccgagaatca tgaaggtggc ggtgtcttcc
     1681 gcgaagcatt cgtgactgtt cgggcacatc gcggacctgc agaccgtatt aagctgatac
     1741 tttttcagga tcgcatgagt gcggaccgtg gaactctccg gcaaggaacg cctgagccaa
     1801 tcaggaaacc tgcggtaata ctgcgccacg aactagggtc ctctcgggtg gatttcgatg
     1861 tgaaaaagag aagaaagttt aataaattat agcggaacgt gtcaaggagt atggcgctta
     1921 cgcgcctcga aacttttcgg cagcttccgg ttaatcacga tacggcgcca aaagctcggg
     1981 agggtgaccg atatcgacga cttcaacccg tccggcaagc gcgggcccct cggccaggaa
     2041 caggcccgct ttgggcagac cgaaggtgac cgtcaccgtt gccttgaccg acgtcccagc
     2101 gacggttccg tcatcagcgt tcagcccgga cggaagatcg gcgctgtaaa cggtttttcc
     2161 cgaagcgttg atcgcacgca cgaccctttc atgaagcccc gaaatcctgc cgcgaaaccc
     2221 gaaaccaaag atcgcgtcca gaacgacctg actctccgga aggatcgcgg ccatgatcga
     2281 ttctgcgtcg tgatcccgca gccgataaaa cgccgcacgc gatcccttca acagattgta
     2341 ttgcagaagg gcctcggggg aaaacaccga cggcggatcg atgagaagaa tactgacttc
     2401 gatatcgcgt tcgaggagat accgggccgc ggcgagaccg tcgccggcgt tgttcccctt
     2461 tccgcaaatg atcagcacct tgcggcatgt cggatcgaga taaatgatcc ttgcgagcgc
     2521 gcggcctgca ttttccatca acgttcggcc ggggatgccg aacttttcgg agctgatgcg
     2581 atcgatctca cgcatccggg ccgacgtgac aggccgcatg gaaggtccgg gacggccgcc
     2641 tcaggtccgc ggaaattgca atttggaagg tgcggacgcc cgcaaggggg attttttctc
     2701 cacctcggga gcatcagtca cgaggaacag cgcgagcttc tgtaatccgg ccttaagggc
     2761 ctcaacaccc cgggcccatt cgccaagctc ccggttcgcg gcggtcagct tgcgttcgac
     2821 acggctgcgc gcccggcggc tttcttcctc gaccttcagg atccgcgcct cttgctcggc
     2881 aaagcggctt tcaaactcct gcagcaatgc ggaacgttcg gcgtcaaatc gggcgttttc
     2941 gcgccgcatt gcttccacct cctgattctg ctgtatcagc tcgccgatct tcttttcgaa
     3001 ttcgtcaagc atccggtcgc gttctttcat ctcggattcc ctcgcgcgga gcatttcatc
     3061 ggcgcggttc agcgcgtgca ccagagagcc gttctgctgc tgaagctcgg cctcccggct
     3121 tcgaagttcg tcgatggtgc gctggatctc atccgaagaa ttctgcttct cccggataac
     3181 ggcctcaagc tcttcgatct ttttgttcag atcacccgtc tgggattgaa gcgtgcggcg
     3241 ttcttcatcg tgtttcgcgc aaagcttttc cacctcagcc tgcatcgact cgagttcggc
     3301 ctggcgatgc ttcgcgatcc cgtccaggtc tccgaccttt ccggtcagct ggctgatctc
     3361 ctgctgaagc gctgcgcgga gattttcgcc ttcggtgatc gcggcatttt tttcatccag
     3421 cgttttttga agctggtctg cccgagcggt cgcgccaact tctttttggg taaactcccc
     3481 gagtcgttgc tgtgcggtcc gaagatccga atctttttgg ccaaggcggg tttccaggtc
     3541 cttgatcgtc tgaaggagag cttcgttttg ctcttttgcc cgctcggcct cccggacggc
     3601 agattcttcg gtcgcgccgg gagaactttg cgcctgctcg cgctcttgcg cgggctgttc
     3661 cgcttgctgc ttctccgctg caagcttcgc ctgcatatcc tccttcaggc gatcctgttc
     3721 ccgggaagct tgtatctgct gctgctgttg ttgttgttgt ttttcatcct ggatcttcgc
     3781 aagccggctc gatagctctt tcgcccgttt ttcccaaagg gaggctggtt ccggcgtcac
     3841 ggcctgttcc tgtgtatccg ccttcggctc cggggccggt tccggtgcgg cggccggctt
     3901 cggagcgctc ctggccggag tttcagccgt cgcgggcgcc ggttccggtg tttgctgccg
     3961 caagaaagag aaagcatgtt ttgcgggcac cggaggtttt tctgccggcg aggactcttc
     4021 gggcttcgtc gctttttccg gagtcgcggg taacaccggg gccggcttgg cgggacgcgc
     4081 gccagccgca ggggcctcgt ccttcaggaa gaacgagagg tccggcatct cggaccagag
     4141 gatctgatag cagttctttt ttatcatttc gtgggaagtc gtctcgacgt tcgcgccgac
     4201 tttggcgaaa aaattcttga ttcgcgcggc agtcgagaac gaaagtccgt ccagaaggat
     4261 caggggggtg ttccggaagg cctgttcagc atctttttta tcaagaccaa aaatgtccgc
     4321 gacctgtttc accagttctt ttctgagttt ggacgggaaa gactggataa tgacacacca
     4381 attctcgttg gaggatacgc ccttcatgtg atcgaaattc atgccggatc cttctgttag
     4441 ttcggtttgc gctgcaagga aagtacggta ttataccgtt tcaaacgaat atttccagca
     4501 gtacctagac ttaaaaacac tcacgtatgc tgacgatcaa agaccgaacc ttcaaaaccg
     4561 cggtcatcca gactccgctc gccggatgca ccgacctcgc cttccgcctc atcgcgcgcg
     4621 atcacgggat ggaactcgct ttcacggaaa tgatttcgtc cgaagcgctt gtccgcgctc
     4681 acaaaaagac ctataccctt ctcaagcggg ccagggaaga cagtcccacg ggctgtcaga
     4741 tcgtggggcc ggaccctgat tccatgggca ggtccgccgc gatcctcgaa ccgctcgggt
     4801 tcgatatcct cgacatcaac tgcggttgcc cggtccgcaa gatcaccggg caggaggcgg
     4861 gcgcggcact cctgaaagac ccgaaacgcg cgggaaagat cttccggagc gtcatccgtt
     4921 cggtcaaagc tgttccggtc accgtcaaga tgcgcaccgg gtacacggac ccgagcggga
     4981 aagaggccgt tcgactcgcc agaatcgcgg aggattgcgg tctgagcgcc gtgacggtcc
     5041 acggacgcac gaaggcgcag ggatattcgg gaaaagcgga ctggcggatc atcgagagcg
     5101 tcaaaaaagt tgtcacgatt cccgtgatcg ggaacggcga cgttttttcg gcggcggacg
     5161 ctcttcggat gcgttcggaa acggggtgcg acgggatcgc gatcggccgc ggctctctcg
     5221 gcaacccgtg gatcttcggg cagatccgcg cggccttcgg tcacaacaag cggtccgtcc
     5281 cgtccttccg ggaggtcaaa cagaccgccc tccggcacat ggacctcacg gcactttggg
     5341 acggagagaa aacggctcac ctgaaatcac ggcatatcgc ctgctggtac ttcaagggtt
     5401 gccccggatc ttcccgcctg cgggaacgca tcaaccggag ccgctccttc gaagacattg
     5461 tatcgatcgt gaaggcctac gagccgcatc gccccgtttg atcccgcgat ccggcggatt
     5521 taccagtgac gccggaccca atcggtagag cgctttgaag acagccggaa cgagatcgag
     5581 tgaatgatct gtttgagcgt ccggtggaga atcttgatca gaagcgcgtt gggccggaaa
     5641 tccgcgggag cgaaatgttc ggcccttttt tccgctatca tctccttgac ttccctcggg
     5701 acctcgcccg acggatcata aacggcgatg gcgtgaccgc cgcttttgtt catcaccgtc
     5761 atggacggca cgtccgtgat cccgtcgccg atgtagatca tgttgctgaa cggcacccgg
     5821 cgtttcacct caggcatatg ctcgttgata cc
//
LOCUS       DAOOBX010000014         5769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_16074_length_5769_cov_2.693385, whole genome shotgun sequence.
ACCESSION   DAOOBX010000014 DAOOBX010000000
VERSION     DAOOBX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16074_length_5769_cov_2.693385"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLY30_00455"
     CDS             complement(<1..581)
                     /locus_tag="PLY30_00455"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64600.1"
                     /translation="MMKFPRLHFLQIAVFAVLAILAVSLFHVQVLQGSYYRGLSTRNH
                     IRLIPMEASRGRVFDRAGRLLATNRAAYDVVAFPEDVTPEVYPRLAKLLGMKEKEVRH
                     QMSAGREYPFAPAVIKKDVAKSLAILIEERKPELPGVAIRVDGVRYYPYGETASHVIG
                     YIGAINMAEYDRLDRDRFGINSMIGRAGIEKFF"
     gene            complement(578..1096)
                     /gene="mreD"
                     /locus_tag="PLY30_00460"
     CDS             complement(578..1096)
                     /gene="mreD"
                     /locus_tag="PLY30_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPW64601.1"
                     /translation="MSRLPTFKIAFFFLFLAILDHSVMPAFRIGNACPSFLYIWICYV
                     SFEWEARKTIPVAFWAGLLKDLMSGGPLGFQTGILVAVAFLLDQTVQRIEREFPGIYF
                     LLTFLFVLLCEASKLVLEIFVGRIHGFVPVHLGVIFLMALYTALLLPVFDILAGFWLG
                     RRSLTRQYELFR"
     gene            complement(1069..1839)
                     /gene="mreC"
                     /locus_tag="PLY30_00465"
     CDS             complement(1069..1839)
                     /gene="mreC"
                     /locus_tag="PLY30_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPW64602.1"
                     /translation="MAAIVILIISFQFPGLNRGVHNLFDSISKPFLAAGSGIRDMATG
                     LRFNFGKFWNAVAREREYQTRILELESKLLGYDELDRENQRLKKLLNFSLTIPCKTIG
                     VRLIGEGSTPWRSVVLLDKGARRGIKADMALVAPEGLAGRVLEAGPLTSRAILLPDPD
                     CRVSALTGTGRVQGVVTGTGTGLLRMKYLALDAEIAIGEEVITSGIESIFPKGIRIGK
                     IESVEKDPDGLHLLATVRPSAPFSKLEEVLCLVSQPSK"
     gene            complement(2015..3067)
                     /locus_tag="PLY30_00470"
     CDS             complement(2015..3067)
                     /locus_tag="PLY30_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPW64603.1"
                     /translation="MLLLDRLLGFFSSDIGIDLGTANTLVYVKGQGVVLCEPSVVAVE
                     RATHQVLAVGEDAKRMLGRTPGNITAIRPMKDGVIADFDITEAMLRYFIRKVHRRHSP
                     VGPRVIVAVPSGITEVEKRAVKDSAERAGARMPVFLVEEPIAAAIGVGLPIHEPAGNM
                     IIDIGGGTTEIAVISLSGIVFAKSIRIGGDEFDDSIINHLKKAYNLMIGERTAEEIKI
                     RIGSAYPLEEETMLDVKGRDILAGLPKTISITSEEVREALSEPLSSILEAVRIALERT
                     PPELSADLIDRGLILAGGGSLLRGLDKLISEETGLPVHIADDPLTAVALGTGRYLSDF
                     SLLKRLSVNSSASREY"
     gene            complement(3158..4453)
                     /gene="aroA"
                     /locus_tag="PLY30_00475"
     CDS             complement(3158..4453)
                     /gene="aroA"
                     /locus_tag="PLY30_00475"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPW64604.1"
                     /translation="MSFLTFNGPGKFSGTVMPPGDKSVSHRALLFGSLSDGESRYENF
                     LEGEDCLRTLEALRMMGISIDHVPGSGVITVRGNGVRGLKAPAGEIYLGNSGTSIRLL
                     LGILAGQEFETTLSGDPSLSLRPMRRVTDPLKRMGAQIKGKDNANFAPLTIRGGNLRG
                     IDFDNRLSSAQVKSALLFAGMYAGGPTVIRETIASRDHTERFLKAAGANFTREGGLLT
                     IGRTDNLRPFRAKIPGDISSAAFFLAGAAMVPGSEILVRDVGLNPTRTGILDVLKRMG
                     AGIEVRVTQEVPEPLGEIRVRGARRRGAGVGRSEIPSLVDELPVLMVAMSLAEGESLV
                     SGAEELRVKETDRIRSMVRNLKAVGASIEELPDGCFIRGVEALRGGAIDSFADHRTAM
                     SLAIASLASNAPIVVNGTDCIATSYPDFERHFYSVFKPR"
     gene            complement(4466..4705)
                     /locus_tag="PLY30_00480"
     CDS             complement(4466..4705)
                     /locus_tag="PLY30_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64605.1"
                     /translation="MMKKILTCIKYAVIVTAIHSLGVSAIAFAEDVPWTDRFMNEFKE
                     IKERIASLEKQQKEILAKEDEILEEIARVRVWARR"
     gene            complement(4710..4961)
                     /locus_tag="PLY30_00485"
     CDS             complement(4710..4961)
                     /locus_tag="PLY30_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64606.1"
                     /translation="MRSGSFKSLFPVMLVFSGVVVCPVAMAAETPWTDRFFQEFDQVK
                     SRLIVAENQRAGILARQEQILKELDSLRIQLRRKPRKKA"
     gene            complement(4942..>5769)
                     /locus_tag="PLY30_00490"
     CDS             complement(4942..>5769)
                     /locus_tag="PLY30_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64607.1"
                     /translation="GIILAFILSLAAAGGAYFLYQNLGAERSARLDLEEKYDASKERV
                     LTLQSEKEQIKAAKEQFEAESEEYQRKAQTFESQVSQLKKQSSQIADAKVTLEEQLRE
                     KEEKLAAMEKRITELQRQAEEAMKACKVTPSDVKATLSGMTAPGTGTTHGGLVFSSPE
                     AAGGSTSSVVAAPAAVTPSVGISSAPAVSSVPASAPVDLSRGTRILTVNRKFNFVVVN
                     MGLKDGLKMGDQLKVVKGGVPSATLQIEKLYDKFAAATILNENAQNQVAEGDEVRKF"
BASE COUNT         1252 a         1743 c         1672 g         1102 t
ORIGIN      
        1 tcaaagaatt tctcgatccc cgcacgtccg atcatcgagt taatgccgaa ccggtcgcga
       61 tcgaggcgat catattcggc catgttgatc gcgccgatat agccgatcac atgggacgcc
      121 gtctccccgt aaggataata ccggacaccg tcgacacgga tcgcgacgcc cggaagttcg
      181 ggcttgcgct cctcgatcag gatcgcgagt gatttcgcca cgtccttttt gatcaccgcg
      241 ggagcgaacg gatattcccg gccggcgctc atctggtggc gaacctcttt ttctttcatg
      301 cccaggagct ttgcgaggcg cgggtagacc tcgggcgtca catcctccgg aaaagcgacc
      361 acgtcatagg ccgcgcgatt cgtcgcgagc aatcgtcccg cgcggtcgaa gacgcgcccc
      421 cgggacgctt ccatcgggat cagacgaata tggttgcgag tgctcagtcc ccggtaataa
      481 ctgccctgaa gcacctggac atgaaagaga ctgacggcca ggatcgcaag caccgcgaac
      541 accgctatct ggaggaaatg caggcgcggg aatttcatca tcggaaaagc tcgtattgtc
      601 ttgtcagcga acggcggccg agccagaaac cggccagtat atcgaacacc gggagaagaa
      661 gcgcggtgta gagcgccatg aggaatatca cgcccagatg gaccggaaca aacccatgga
      721 tccggccgac gaagatctcc aggacaagtt tggaggcctc acagagaagg acaaacagga
      781 acgtgagaag gaaatagatt cccgggaact cgcgctcgat ccgctggacg gtctggtcca
      841 gcaggaacgc gaccgccacg aggatcccgg tctggaaccc gagaggcccg ccgctcatga
      901 ggtccttgag cagccccgcc cagaacgcca cggggatggt cttcctggcc tcccactcga
      961 aagaaacgta gcagatccag atgtaaagga acgaggggca ggcgttcccg atgcggaagg
     1021 ccggcatgac gctgtggtcc aggatcgcga gaaaaaggaa aaagaaagct attttgaagg
     1081 ttgggagacg agacacagga cctcctcaag cttggaaaac ggcgcagacg ggcggacggt
     1141 cgcgagcaga tgcaggccat cgggatcctt ttccacggat tcgatcttgc cgatccggat
     1201 gcctttcgga aagatacttt cgatcccgga ggtgattacc tcttccccga tagcgatctc
     1261 ggcatcaagg gcgaggtatt tcatccggag cagtcccgtg cctgtccccg tcacaacccc
     1321 ctggacccgc cccgtgcccg ttagcgcgct cacgcggcaa tcgggatccg gcagaaggat
     1381 cgcgcgcgat gtcagcgggc cggcctccag gacgcggccg gccagccctt cgggagcgac
     1441 aagagccatg tcggccttga ttccccgcct ggcgccttta tcgagcagca ccacgctcct
     1501 ccacggcgta ctgccttcgc cgatgagacg gactccgatg gtcttgcaag ggatcgtgag
     1561 cgaaaaattg aggagttttt tcagccgctg gttctcgcga tcgagctcat catagccgag
     1621 caatttactt tcaagctcga ggattcgggt ctgatattca cgttcacgcg cgacagcatt
     1681 ccagaatttc ccgaaattaa aacggaggcc ggtggccata tcacggatcc cggaaccggc
     1741 ggcgaggaac ggtttggaga tggaatcgaa aaggttatgg accccgcgat tgagaccggg
     1801 gaactggaag gaaataatga ggatgacaat ggcagccacg aaccaaccca acgaacgcca
     1861 gaagcgcatt gaaacaaatc tcccgggaat gagaggccga aactaaaaag ttatgaacga
     1921 aggacgaaac agagggagac cgttcggcga gacccgaaaa agccttgaga ccgacccgcg
     1981 tgtcagccgc gcagggtgcg gagtggccga ccgatcagta ttcccggctc gcactgctgt
     2041 tgaccgacaa ccgcttcagg agactgaagt cgctcagata gcgccccgta ccaagcgcta
     2101 ccgcggtcag gggatcatcc gcaatatgaa cgggaagacc cgtttcttca ctgatcagtt
     2161 tatcaaggcc ccggagaagc gagccgcccc cagccagaat caggccccgg tcgatcagat
     2221 ccgcggaaag ttccggcggc gtgcgttcca gggcgatccg gacagcttca aggatcgagg
     2281 aaagcggttc ggaaagcgcc tcacgaactt cttcgctcgt gatactgatc gttttcggaa
     2341 ggcccgcgag aatatcgcgg ccttttacat ccagcatcgt ctcttcttca agcggatacg
     2401 cggacccgat ccggatcttg atctcttctg cggtccgttc accgatcatg agattatagg
     2461 cttttttcag atgattgatg atcgagtcat cgaactcgtc gccgccgatc cggatacttt
     2521 tcgcgaacac gatcccggaa agcgagatca cggcgatctc ggttgtgccg ccgccgatat
     2581 cgatgatcat gttgccggcc ggctcatgga tcggaagacc gacgccgatg gcggccgcga
     2641 tgggttcttc gacgaggaaa acgggcatcc gcgcgccggc gcgttcggcg gaatccttca
     2701 cggcacgctt ttcgacttcc gtgataccgc tcggaacggc cacaataaca cggggaccga
     2761 cggggctgtg acggcggtgg accttgcgga taaaataccg gagcatcgct tcggtgatgt
     2821 cgaaatcggc gatcacgccg tccttcatcg ggcgaatggc ggtgatattg cccggcgtgc
     2881 ggccaagcat gcgtttggcg tcttcgccga cggcgagaac ctggtgggtc gcccgctcga
     2941 ccgcaacgac ggaaggttcg caaaggacca cgccctgccc cttgacataa acgagcgtat
     3001 tggcggtgcc gagatcaatg ccgatgtctg aagaaaagaa ccctaagagt ctgtccagca
     3061 ataacatgtg agcgcctgtt tccttcgttt gagggaccaa gttttacagg gaggtagtat
     3121 atgtcaaagc cgtcagccgt gtcaaaccaa aacccgttca gcgcggcttg aaaacagaat
     3181 aaaaatggcg ttcgaaatcc gggtacgacg tcgcgataca atcagtgccg ttcacaacga
     3241 tcggagcgtt cgaagcaagg ctcgcgatgg ccagactcat cgcggtccgg tgatccgcaa
     3301 agctgtcgat cgccccccct ctgagcgctt cgacgccccg gatgaaacac ccatccggaa
     3361 gctcctcgat cgaggccccg acggccttca gattccggac catggaccgg atccggtccg
     3421 tttccttgac gcgcagctct tcggcgccgg aaacgaggct ctcgccttcg gcaagagaca
     3481 tcgcaaccat cagcacggga agttcatcga caagcgacgg gatttccgag cggcccaccc
     3541 cggcccccct gcgccgcgcc cccctgacgc ggatttcgcc caaaggctcc ggaacttcct
     3601 gcgtaacgcg gacctcgatc ccggcgccca tccgcttcaa cacatcgagg atccccgtgc
     3661 gcgtgggatt gagcccgaca tccctgacaa ggatttctga accgggaacc atcgccgccc
     3721 ccgcgagaaa gaacgccgcc gaggaaatat cgccgggaat cttcgcgcga aacggccgga
     3781 ggttgtccgt gcgtccgatc gtgaggagac cgccctctcg cgtgaagttc gcgcccgcgg
     3841 ctttcaaaaa acgttccgtg tggtctcgcg acgcgatcgt ttcgcgaata accgtcgggc
     3901 caccggcata catcccggcg aagagcagtg cggatttgac ctgggcgctc gacaacctgt
     3961 tatcaaaatc gatcccccgg agattacccc cgcggatcgt gagaggagcg aaattcgcgt
     4021 tatccttccc cttgatctgc gcgcccatcc gtttgagcgg atcggtcaca cgacgcatcg
     4081 gccgcaacga aagcgacggg tcccccgaaa gggtcgtttc aaattcttgt ccggcaagaa
     4141 tccccagaag caggcggatc gatgtccccg aattgccgag ataaatctcc ccggccgggg
     4201 ccttcaagcc gcggaccccg ttcccgcgaa ccgtgatgac gccgctgccg ggaacgtgat
     4261 cgatagagat ccccatcatc cggagggctt cgagcgtgcg caggcagtct tcaccttcca
     4321 ggaaattttc atagcggctt tcaccgtcgg aaagggagcc gaaaagcagg gctcggtggg
     4381 atacggactt gtcgccgggc ggcatcacgg tccccgaaaa cttcccgggg ccgttaaagg
     4441 tgaggaagct catcggaacg cgggtttagc ggcgcgccca gacacggaca cgcgcgatct
     4501 cctcgagaat ctcgtcttcc ttggccagaa tctctttctg ctgcttctca agggaggcaa
     4561 tgcgttcctt gatctccttg aactcgttca tgaaccgatc cgtccacggg acatcctccg
     4621 cgaaagcgat cgcggacacg ccgagcgagt ggatcgccgt gacaatcacg gcgtatttga
     4681 tacaggtcag gatcttcttc atcataaggt catgccttct tccggggttt acgacgcaac
     4741 tgaattctga gactgtccag ttctttgaga atctgctcct gtctggcaag aatcccggcc
     4801 cgctgattct cggcaacgat cagccgggac ttgacctgat cgaactcctg gaaaaaccgg
     4861 tcggtccagg gcgtctctgc ggccatcgcg acgggacaaa ccaccacgcc ggagaacacc
     4921 agcatcacgg gaaaaaggct cttaaaactt ccggacctca tcgccttcgg ccacctggtt
     4981 ctgggcattt tcattgagga tcgtggccgc cgcgaacttg tcataaagtt tttcgatctg
     5041 cagggtcgcg ctgggcactc cacccttcac aaccttcagc tggtctccca tcttgagacc
     5101 gtccttgagc cccatgttca cgacgacgaa attaaacttc cggttcacgg tcaggatacg
     5161 cgttcccctg gagagatcca cgggggccga agccggcacg gaggaaaccg cgggggccga
     5221 cgagattccg accgaaggcg ttacggccgc gggcgccgcc acaacggaag aggtgctccc
     5281 ccccgcagcc tcgggagaac tgaagacgag tccgccatgc gtcgttcccg taccgggcgc
     5341 ggtcatccct gaaagcgtcg ccttcacatc ggacggcgtg accttgcagg ccttcatggc
     5401 ctcctcggcc tgacgctgga gttcggtgat ccttttttcc atcgcggcga gtttttcttc
     5461 tttttcacgc agttgttctt cgagcgtgac tttcgcgtcc gcgatttgcg aactctgttt
     5521 tttcagctgg ctcacctggg attcgaaagt ctgggccttg cgctggtact cctcgctctc
     5581 ggcctcgaat tgttccttgg cggccttgat ctgctctttt tcggactgga gcgtcaggac
     5641 cctctccttg gacgcgtcat atttttcttc gaggtccagg cgagcgctcc gctcggcgcc
     5701 caggttctga tagaggaaat aggcgccgcc ggcagcggca agagaaagga taaatgccag
     5761 gatgatacc
//
LOCUS       DAOOBX010000015         5673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_16402_length_5673_cov_3.184407, whole genome shotgun sequence.
ACCESSION   DAOOBX010000015 DAOOBX010000000
VERSION     DAOOBX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16402_length_5673_cov_3.184407"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..619
                     /locus_tag="PLY30_00495"
     CDS             308..619
                     /locus_tag="PLY30_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW64608.1"
                     /translation="MAYVITEKCHGERYGICATVCPVEAISPGTYQGKDFMVIDPTVC
                     ISCGACAAECPVGSIVESEDMDPTWARINAELAPQFKANPKVTPRSRDEAPKNATNKL
                     K"
     gene            694..1281
                     /locus_tag="PLY30_00500"
     CDS             694..1281
                     /locus_tag="PLY30_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64609.1"
                     /translation="MSRAVSHPDIGELERILKKYAQDFAVSNPAAKAIFRGITVIGVG
                     VRPILDHFLFRTRQPAERVLEFTALGYEKDSSAIVLGHPKNAVSVYRCRCLPAVLIEE
                     AQDRNSLEWVEEFGDSAPYVMAVRVDDIEESTFHLEKQAVQFLRPGAGKSGEEVRAIA
                     AMPFPRNGRASTVLVLAERHAGNMSYYAPDFWNKA"
     gene            1313..1672
                     /locus_tag="PLY30_00505"
                     /pseudo
     CDS             1313..1672
                     /locus_tag="PLY30_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
     gene            1850..2311
                     /locus_tag="PLY30_00510"
     CDS             1850..2311
                     /locus_tag="PLY30_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPW64610.1"
                     /translation="MAQSQPRPGWDEYFMNIAKVAALRSNCMKRKVAAVIVKDKRIIS
                     TGYNGTPRGITNCNEGGCPRCLSIETSGKDLGECLCSHGEENAIVQAAYHGVALKGAT
                     LYTTFSPCLMCTKMIINAGISEVVYQATYPLSDISFKLLQQAGVTIRQLAA"
     gene            2423..3565
                     /locus_tag="PLY30_00515"
     CDS             2423..3565
                     /locus_tag="PLY30_00515"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPW64611.1"
                     /translation="MRDKGKATGNEHPEVNNASLLIERIRGNDFRIKQGRVEIFLARE
                     FGFCYGVERAVTYAYETRAKFPDRRIFLTDEIIHNPRVNKELRELGIQFLSGAAACGV
                     STADLTAKDIVVMPAFGLPVEALKKYQERGCVIVDTTCGSVMSVWRRVESYARDGFTS
                     VIHGKYDHEETRATCSRAGKYIVIKDTEQAKLVCEQIRGPGNRDSFPGKFGDVCSAGF
                     DPEQDLGKIGFANQTTMLMSESVEISGMLRAALATRYGEAEAEDRFRHFDTICSATQD
                     RQDAIRELGEKGMDLILVIGGYKSSNTGHLAGIASGYARSFHIEDADCLLSAEKISHR
                     GIDTHVITETVDWLPAGPVRIGITAGASTPNRVVEEVIERLVKITG"
     gene            3646..4065
                     /locus_tag="PLY30_00520"
     CDS             3646..4065
                     /locus_tag="PLY30_00520"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPW64612.1"
                     /translation="MEKLKDSWDWRWQPFRGRKRRQHKCSHLHGHSAKVRIVMQTPKL
                     NRRGMVVDFYDIKRTIGDWIDRTLDHKMILNSRDPLAPILKKAGEPVILFKGNPTAEA
                     IAEWIFREARRMKLPVVEVALWESGDSMAVYSANPKS"
     gene            4191..>5673
                     /locus_tag="PLY30_00525"
     CDS             4191..>5673
                     /locus_tag="PLY30_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64613.1"
                     /translation="MTKTLLIGPAGCGKTRALLEAFEKSLRTSNPLEPDTFLIVPSAE
                     HTERVVSLLIQRGLPGFFHKRVTTLSRLITDVFRVPEIPVVSSLTRAIIVRDLLGKND
                     WEYFRDVKDLPGFVGLLLGGISELKEACVTPAEFRERMNALKKYEPAFGPKYEALAAV
                     YEGYESELKERGLRDLQDTLRIWRERTRKGGQPPVRFKALWLDGFFDFSNLQAEYVRA
                     LAALSDEMIVTLTAEEGPVREDAFETIRSTRAMLEEAGFKVEKMKPVCHRTREPALLH
                     LQRNLFGQNIKTGSGTISGDRNGARPPEGITFLDAVGIEGEIELIAREIHGLYASGNY
                     RYSDFAVLFRQVRDYGRVVPPIFERYGIPVEVHERDRLKLSPWIGAVAALLSIFRNGW
                     RREDVLAFVKSGYVRKFGDLARDNDWLSKFEAGSFENGVLEGREAWTAPWVNETALAE
                     NGGAGKDKFLGVLLELEAAFLAAETAADHIRLLTHAVGHTFGIL"
BASE COUNT         1208 a         1593 c         1757 g         1115 t
ORIGIN      
        1 agcccgccac ggcgcatctt ttcatcatga acccgctttc gggaaaggga tggtcggccc
       61 ttttcagcac gcatccgccg gtcggggacc gtattgcccg tctggaggat cagtcccggt
      121 gttccataag aaaacaatgt ccagaggggt gattttttta gcgcataccc gaaagatttg
      181 agtcagacgt gtctcgattc cttgtgttcc cgcgaatgaa cggccggccg aaaacattga
      241 cgatggaggg ggatgaaagc tagaatacgc cccattcggg aaactaacca agaggagaaa
      301 atgaacaatg gcttacgtca tcactgagaa gtgtcacggc gaacggtatg gtatctgcgc
      361 gaccgtttgt ccggtcgagg cgatttcccc ggggacctac caggggaagg atttcatggt
      421 catcgacccg acggtctgta tttcgtgcgg cgcgtgtgcg gcggaatgcc cggtcggttc
      481 gatcgtggag tcggaggata tggaccccac ctgggccagg atcaacgcgg agcttgcgcc
      541 ccagttcaag gcgaatccga aggtcacgcc gcgatccagg gatgaagctc cgaaaaacgc
      601 cacgaacaag ctgaaataag cggttgtccg aatgaaggcc cgcccgggaa ccgccgggcg
      661 ggcttcttgt tttccaatgt gaaaggtccc ggcatgtcgc gagcggtgtc ccatcccgat
      721 atcggtgagc ttgaacggat cctcaaaaag tacgcgcagg attttgccgt cagcaaccct
      781 gccgcgaaag cgatttttcg cgggatcacg gtcatcgggg tcggcgtcag gcccatcctg
      841 gaccatttcc tcttccggac ccggcagccc gcggagcgtg tcctggagtt caccgcgctc
      901 ggttacgaga aagattcctc cgccatagtt ctcggtcatc cgaaaaacgc cgtttccgtc
      961 tatcgttgcc gatgtcttcc cgccgttctc attgaggagg cgcaagacag gaattccctg
     1021 gagtgggtcg aggaattcgg ggacagcgcg ccttacgtga tggcggtccg ggtcgacgat
     1081 atcgaggagt cgacctttca tctcgagaag caagctgtgc agttcctccg gccgggcgcg
     1141 ggcaagtccg gagaagaggt ccgcgcgatc gcggcgatgc cttttccccg gaatggccgc
     1201 gcttcaacgg tgctggtcct ggcggagcgc catgccggga atatgagtta ttacgccccg
     1261 gatttctgga acaaggcctg aagcgtgtca ctttagcatt tggagatttc acatgatcat
     1321 cggactgacg ggaaagaatg gctcgggaaa aggcgaggtc gcgaattttc tgaaagagcg
     1381 cggcttccat tactattcgc tttcggacgc cattcgcgag gaggcgaaaa aacgcggggt
     1441 tgagatcagt cgcgataccc tgatcgcgct cggcaacgag ctgcgcgaaa aagaaggccc
     1501 cggatgcctg gccgagcgca tcttcgcgaa gctggatccc gaaaaacatt acgtgatcga
     1561 ttccatccgc catccttccg aggtccgggt gttccgccgc cgtccgaatt tcacatttct
     1621 ttgcgtccgg gcgcccgaac ggcttcgttt cgagcggctc cgtcagcgcg gccgcgagaa
     1681 cgatcccggg gtctgggagg attttcaggc catcgaggcg aaagaggcca aaagcgagaa
     1741 ccggaccgac cagcagctcg accaggcgct gatgctcgcg gatatccagg tcgacaataa
     1801 cggccccctg aaagagctgc atgataaggt gaaacagatt ctcgtgaaac tggcgcaaag
     1861 ccagccgcgc cccggttggg acgagtattt catgaatatc gcgaaggtcg cggcgcttcg
     1921 gtcgaactgc atgaaacgca aggtcgcggc cgtgatcgtg aaggacaagc ggatcatttc
     1981 caccggttac aacggcacgc cgcgcggtat tacaaattgt aatgaaggcg gctgtccgcg
     2041 gtgcctgagc atcgagacat cggggaagga tctgggagaa tgcctctgtt ctcacggcga
     2101 ggagaacgcg atcgtccagg cggcctatca tggtgtcgcg ctgaagggag cgaccctgta
     2161 cacgaccttt tcaccgtgcc tgatgtgcac gaagatgatc atcaacgccg ggatcagcga
     2221 ggtcgtttac caggcgactt atccgctctc cgatatttcc ttcaagcttc tccagcaggc
     2281 cggggttacg atccgtcagc tcgccgcctg atcccgatgg ggtcttcggt gccccggacg
     2341 tggagagcgg gtgttttcgg ccgacctcgt gacaagcatt caacaaacga tcctgggcca
     2401 cgattgcaac gggcgaacca ttatgagaga taaagggaaa gcgaccggga acgaacatcc
     2461 tgaggtgaat aacgcgagcc ttctgatcga gcggatccgg gggaatgatt tccggatcaa
     2521 gcaggggcgg gtcgagatct tcctcgcgcg cgagttcggg ttctgctacg gtgtggagcg
     2581 cgccgtgacc tatgcttatg agacccgcgc aaaatttccg gaccggcgta tctttctcac
     2641 cgatgagatc atccacaacc cgcgcgtgaa caaggaactt cgcgagctcg gcattcagtt
     2701 cctgtcgggc gcggcggctt gcggagtttc gacggcggac ctgacggcga aggacatcgt
     2761 ggtcatgccg gccttcgggc ttccggtcga agcgctcaag aaatatcagg aacgcggctg
     2821 tgtgatcgtg gacacgacct gcgggtcggt catgagcgtc tggcgccgcg tggaatccta
     2881 tgcgcgggac gggttcacct ccgtgatcca cggtaaatac gatcatgaag agacgcgcgc
     2941 gacgtgctcg cgggcgggga agtacatcgt tatcaaggac acggaacagg cgaaactggt
     3001 ttgcgaacag atccggggac cggggaaccg ggattcgttt cccggcaagt tcggggacgt
     3061 ttgctcggcg gggttcgatc ccgaacagga tctcgggaag atcggtttcg cgaaccagac
     3121 gacgatgctg atgagcgagt ccgtcgagat ctcgggcatg ctgcgggccg cgctcgcgac
     3181 ccggtatggg gaagccgaag cggaggacag gttccgccat ttcgacacga tctgttccgc
     3241 cacgcaggac cgccaggacg cgatccgcga gctcggcgag aagggaatgg acctgatcct
     3301 cgtgatcggc ggttataaat cgagcaacac gggccatctt gcggggatcg cttccgggta
     3361 cgccaggtcc ttccatatcg aggatgccga ctgtcttctt tccgcggaga agatcagcca
     3421 ccgcggcatt gacacgcacg tcatcaccga gacggtggac tggcttccgg cgggaccggt
     3481 gaggatcggc atcaccgcgg gggcctcgac ccccaaccgg gtcgtcgagg aagtcattga
     3541 acgcttggtc aagataacgg ggtaaatcat gtttaaagtc gcaaaaacac tttatttcaa
     3601 ttacggacac cggttgctcg ggggccatga caagtcccgg tctccatcga aaaactcaaa
     3661 gattcgtggg actggcgctg gcaacctttt cgcggaagaa aacggagaca gcacaagtgc
     3721 tcgcaccttc acggccacag cgccaaggtc cgcatcgtga tgcagacgcc gaagctgaac
     3781 cggcggggta tggtcgtgga tttttacgat atcaaacgca cgatcggcga ctggatcgac
     3841 cgcacgctgg accacaagat gatcctgaac tccagggatc cgctcgcccc gatcctcaaa
     3901 aaagcggggg aaccggtcat tctcttcaag gggaacccga ccgcggaagc cattgccgaa
     3961 tggattttca gggaagcccg ccgcatgaaa ctgccggttg tggaggttgc cctctgggag
     4021 agcggcgaca gtatggcggt ttattcggca aatccgaaat cctgatttca gatgacaaaa
     4081 caatgatcca ggcccggacg tttaaaaaac aaaatcgatc cctgtccgaa attctcccgg
     4141 agcccggcat ccggactccg gatccggaaa ttcgggtttg agcttttgcc atgacgaaga
     4201 ctctcctgat cggcccggcg ggatgcggga agacccgcgc gttgctggag gccttcgaaa
     4261 aaagcctccg gacgtcgaat ccgctcgagc ccgacacttt cctgatcgtc ccctctgccg
     4321 agcacaccga acgggtggtt tcactcctca tccagcgggg acttccgggt ttttttcata
     4381 aacgcgtcac gaccctttcc cgccttatca ccgatgtctt ccgggtcccg gagattccgg
     4441 tcgtttcaag ccttacgcgg gccattatcg tccgcgatct cctcggcaag aacgactggg
     4501 aatattttcg ggacgtgaaa gacctgccgg gattcgtcgg acttctcctg ggcgggattt
     4561 cggagctcaa ggaagcctgc gtgacgcccg cggaattccg cgagaggatg aacgccctca
     4621 aaaaatatga accagccttc ggccccaaat acgaggcgct tgccgcggtc tatgaaggat
     4681 atgagtcgga gctgaaggag cgcggtctca gggatctcca ggacacgctc cggatctggc
     4741 gggagcggac ccgcaaaggc gggcagccgc cggtccggtt caaagcgctc tggctcgatg
     4801 gctttttcga tttctccaac ctccaggccg aatatgtccg ggcactcgcc gccctgtcgg
     4861 atgagatgat cgtgacactg acggcggaag agggcccggt tcgtgaggac gctttcgaga
     4921 ccatccgttc cacccgtgcg atgctcgagg aagccggttt caaggtggaa aagatgaaac
     4981 ccgtttgtca ccggacgagg gaaccggcgc ttttacatct tcagcggaat ctgttcggcc
     5041 aaaatataaa aacggggtca ggcaccattt caggggaccg caacggagcc agacccccgg
     5101 aggggatcac ttttcttgat gcagtcggaa tcgaaggcga gatcgagctg atcgcacgcg
     5161 agatccacgg gctttacgcg tcggggaact accgttacag cgatttcgcg gtgctgtttc
     5221 gtcaggtccg cgattacgga cgggttgttc ccccgatctt cgaacgctac gggattccgg
     5281 tcgaggtcca tgaacgcgac cgcctcaagc tctcgccctg gatcggtgcg gtggcggcgc
     5341 ttctttcgat attccggaac ggttggagac gggaggatgt tctcgcgttc gtcaaatccg
     5401 gatacgtgcg gaaattcggc gatctcgcga gggacaacga ctggctctcg aaattcgagg
     5461 cggggagctt cgagaacggt gtcctcgaag gccgcgaagc gtggacagcc ccctgggtta
     5521 atgagacggc gcttgcggag aacggagggg cgggcaagga caaattcctc ggggtcctgc
     5581 ttgagctaga ggccgctttt ctcgcggcgg aaaccgcggc cgatcatatc cgtcttctca
     5641 cgcacgcggt cggccacacc ttcggcatcc tcg
//
LOCUS       DAOOBX010000016         5459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_17154_length_5459_cov_3.578830, whole genome shotgun sequence.
ACCESSION   DAOOBX010000016 DAOOBX010000000
VERSION     DAOOBX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17154_length_5459_cov_3.578830"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLY30_00530"
     CDS             <1..835
                     /locus_tag="PLY30_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPW64614.1"
                     /translation="ADLASGRDVLDHVKTSIGDLFPSGQTLKDFSAELAYSDGQWDVP
                     KFHAQVYGGMLDLAGSLDLSAKPIGYLCEGEAKGLDFASFLTRSGQEQPPMTGSLTLK
                     FSLHGNGWGKDNWAGLLGGKGRFTLSNGRVNFIDLPGAVATITPFSGIRERLKDPGAF
                     SELDFNWSLFDGKVMTDNFLTRGSDYVVAGEGTLGFDGLTNFRWDVFFPTTLAAEIFP
                     EMAGTFRRETRAHLGPITMLVTGPLSAPEIKPDPAQVEELVEKIRNKKARALLFELVL
                     D"
     gene            complement(882..1592)
                     /locus_tag="PLY30_00535"
     CDS             complement(882..1592)
                     /locus_tag="PLY30_00535"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HPW64615.1"
                     /translation="MKSMKKTPQITKSLLKKSFGLTVKKVPLYEAALTHPSFRHENQI
                     KAKLEDFDRLEFLGDSILNEVICQRIYADFPDADEGMLSKLRSILVSQKILSRIAAKF
                     KLHNLIRLGNSLGQAMKTPYKFKLMTDSMESIFAAVFFDLGRKKTEAFILKHYAEYID
                     AKRLFRLDPNPKSTLQELTLKKWQKLPQYTHKFGPKGVTTTVSIDRRRKASGFHKIKK
                     ESELKAARALLKLLRQEQ"
     gene            1795..2820
                     /gene="argC"
                     /locus_tag="PLY30_00540"
     CDS             1795..2820
                     /gene="argC"
                     /locus_tag="PLY30_00540"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPW64616.1"
                     /translation="MLNCAVLGATGYTGAELTRLLLSHPDVRITHLTTRQEKPIPLSE
                     VLPSVPRGVQLQITPYDFEDLRKKADMVFLCLPHTEAMTTGDLFLQAGKVVIDLSADF
                     RLKDATLYPKWYGIDHAFPARLRQAVYGLCEVNRKKIRKAELISNPGCYPTGPSLALI
                     PLLEKKLIKHDPVIINAASGVSGAGKKLVTSTQFCEVDGNYNAYKVNKHQHIPEIEQN
                     LSLAAGREVAITFIPHLLPVYRGILATIYVEPAKKTVTEDEVRRALVTAYGKDPFVRV
                     KPAGQFPSLKDVRNTNFCDIGVSLDARTGRLVIITAIDNLLKGASGQAVQNMNIRFGF
                     PEGAGLI"
     gene            2899..4119
                     /gene="argJ"
                     /locus_tag="PLY30_00545"
     CDS             2899..4119
                     /gene="argJ"
                     /locus_tag="PLY30_00545"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPW64617.1"
                     /translation="MKKPFWKREKNGSVTSPRGFVAGGIHAGIKKKKKKYDMSLIFSK
                     IPCVAAGTFTTNRAKAWPVLYSMKVINNARHHAILGTSGNANCLNGAIGKKAVDEITW
                     ETAKALEVLPNEVLIAQTGIIGVPFPVTRVKRAIPKLVAKLSDTGGHGAAKGILTTDL
                     SNKEIALSIELGGKKVTIAACAKGAGMVHPNMATMLCFITTDCAITKPMLRRAVRHAA
                     DDTFNSMAIDNDMSTNDMVLCLANGLAGNPTIAKVDRDYWMFREALEEVMRYIAYEMV
                     KDGEGVTHVCKLRLSGAKTPTDAERAARQIANSMLFKTMLAGSNPNWGRIAAAVGASG
                     VDFNPNHLSISFGDAEVVHLGKLRVLNIPKARRILQKKEYTIDVKIGKGRGRAEFITS
                     DLTTKYVLINAAYS"
     gene            4194..5048
                     /gene="argB"
                     /locus_tag="PLY30_00550"
     CDS             4194..5048
                     /gene="argB"
                     /locus_tag="PLY30_00550"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPW64618.1"
                     /translation="MQELIKKAGVLIEALPYIQKFRGKEVLIKYGGAALTYQEVRDMV
                     LQDLVFMSYVGIRPVLIHGGGKYITENMKKKGIQSQFINGLRVTDKPAISVVNETLAE
                     VNRGIVHDIERLGGSAVGLSGYDAKVLSVVKNTEHGDIGFVGDVSSVNTVPIKELTGF
                     GKIPVIFPVGKGPDGEIYNVNADEAAANIAAAMQVQKFMVLTNVRGILRDFKDESTLI
                     PSLCVGDTQRLIDQQIISGGMIPKVKACIDALEGGVPKAHIIDGRIPHALLLEIFTDQ
                     GIGTEITL"
     gene            5118..>5459
                     /locus_tag="PLY30_00555"
     CDS             5118..>5459
                     /locus_tag="PLY30_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW64619.1"
                     /translation="MKTTDVQKLYNEYVMPTYTRLPVCLVKGKGSRVWDLEGKEYLDF
                     FPGWGVSCLGHCHPAVINAIKHQSRTMLHIPNNFLNIKQSELASEIVKASFPGRVFFG
                     NSGAEANEGAIK"
BASE COUNT         1215 a         1469 c         1624 g         1151 t
ORIGIN      
        1 ggccgatctc gcctccggca gggacgtgct cgaccatgtg aagacctcga tcggagactt
       61 gttcccctcg gggcagacgc tcaaggattt ttcggcggaa ctggcttatt cggacgggca
      121 atgggatgtt cccaagttcc acgcccaggt ttatggcgga atgctggatc tggcggggtc
      181 gctggacctt tcagccaaac cgatcgggta cctttgtgaa ggcgaggcca aggggctgga
      241 ctttgcatcc tttctgaccc gctcgggcca ggaacagccg ccgatgacgg gatcgcttac
      301 cctgaagttc tcattgcatg gaaatggctg ggggaaagac aactgggctg gtttgctcgg
      361 cggaaagggt cgttttactc tttcgaacgg ccgggtcaat ttcattgatc tgcccggcgc
      421 tgtggcgact attacgcctt tctccggtat ccgtgaacgg ttgaaagacc cgggcgcgtt
      481 cagcgaactg gatttcaatt ggagtctgtt cgacggcaag gtcatgacgg ataacttcct
      541 gaccaggggt tccgattatg tcgtggctgg ggaaggtacg cttggtttcg atggcctgac
      601 gaatttccgg tgggatgttt tttttccgac gacccttgcc gcggagattt ttccggagat
      661 ggctggaacc tttcggaggg aaacgcgtgc ccatctgggg ccgatcacga tgctggtcac
      721 gggcccctta tccgctcccg aaatcaaacc ggaccccgcg caagtcgaag aactcgtcga
      781 aaagatccgg aacaaaaaag cccgcgctct ccttttcgaa ctcgttctgg attagaataa
      841 tggggtcagt caccgtttta gggaaacggt gactgacccc attattgttc ctgccggaga
      901 agtttcagaa gggcccgtgc cgccttcagc tccgattcct tcttgatctt gtgaaatccc
      961 gaagccttgc gccggcggtc tatggacact gtcgtagtca cgcctttcgg accgaacttg
     1021 tgggtgtatt ggggaagttt ctgccatttc ttgagcgtca gttcctggag cgtgctcttc
     1081 gggttgggat caaggcggaa tagccgtttg gcgtcgatat attccgcgta atgtttcagg
     1141 atgaatgcct ccgttttttt ccggccgaga tcgaaaaaga ccgccgcaaa gatcgattcc
     1201 atcgagtctg tcatcagttt gaatttatac ggcgttttca tggcctggcc caggctgttc
     1261 ccgagccgga tcagattgtg aagcttgaat tttgctgcga tgcggctgag gatcttctgc
     1321 gaaacgagta tcgaccggag cttcgaaagc atgccctcgt cggcgtccgg aaaatccgcg
     1381 tagatcctct ggcagatcac ttcgttcagg atcgagtccc cgagaaattc cagacggtcg
     1441 aaatcctcga gtttggcctt gatctggttt tcatgccgga aggaagggtg tgtcagcgcg
     1501 gcttcgtaaa gagggacttt tttgaccgtc aggccgaatg acttcttgag aagggacttg
     1561 gtgatctgag gtgtcttttt catggatttc ataagataga gatttaacct ctgaatgtca
     1621 atcaaaatac acgtaacctg attcgtggta tggagttatg agtgttttga gccgcggtcc
     1681 atccccggtt tttgacgtaa cttcttaaat tgacaaagga tagagctatt cctataataa
     1741 cgcgttctca aggaggctgt ccctttattc ccgcaaagga gaaaccgacg tcagatgttg
     1801 aactgcgctg tcttgggcgc tacgggttac acaggcgctg aactcacgag gttgttgctt
     1861 tcccatccgg atgttcggat cactcatctc accactcggc aggaaaagcc gatcccgctt
     1921 tctgaggtcc ttccgtccgt tcccaggggc gtgcaactcc agatcacccc gtatgatttt
     1981 gaagatctca ggaagaaggc cgacatggtt tttctctgcc tgccccacac ggaggcgatg
     2041 acgaccgggg acctgtttct gcaggcggga aaggttgtga ttgatctctc cgcggatttc
     2101 cggctgaaag acgcgacgct ttatccgaag tggtacggaa tcgaccatgc gttcccggcg
     2161 cggctccggc aggccgttta cggactttgc gaagtgaacc ggaagaagat caggaaggcc
     2221 gagcttattt ccaatcccgg gtgttatccg acaggccctt cgctcgcgct cattccgctc
     2281 ttggagaaga agcttatcaa gcatgatccc gtcatcatca atgccgcgtc cggcgtgagc
     2341 ggcgcgggga agaagcttgt cacctcgaca cagttctgcg aagtggacgg gaactacaac
     2401 gcgtacaagg tcaataaaca ccagcatatt cccgagatcg agcagaatct ttcgttggcg
     2461 gccggccgcg aggttgcgat cacgttcatc ccgcatcttc ttcccgtgta ccgcggtatt
     2521 ctcgcgacga tctatgtcga gcccgccaaa aagacggtga cggaggatga agtgcggcgg
     2581 gcgctcgtga ccgcgtatgg caaggacccg ttcgtgcggg tcaagcctgc gggacagttc
     2641 ccgagcctga aggacgtccg gaacacgaat ttctgcgaca tcggcgtttc gctcgacgcc
     2701 cgcacggggc ggcttgtcat cattacggcc atcgacaatc ttttaaaggg cgcctccggt
     2761 caggcggtcc agaacatgaa catccggttc ggtttccccg agggtgcggg gctcatctga
     2821 aaccgcttca cgatatgatc gctgtgaacg aagtgcgaaa accgctatgc aagtcaatct
     2881 aagggagagc agttctcgat gaaaaaacct ttttggaaga gagaaaaaaa cggttcggtg
     2941 acttccccgc gcggtttcgt ggcgggcggc attcacgcgg gcatcaaaaa gaagaaaaaa
     3001 aaatatgaca tgtcgctgat cttctcgaag attccgtgtg tcgcggcggg aacgttcaca
     3061 accaaccgcg cgaaggcgtg gcccgtgctg tattccatga aggttatcaa caatgcgagg
     3121 caccacgcca ttctcgggac gagcgggaac gcaaattgcc tgaatggggc gatcggcaag
     3181 aaggcggttg acgaaatcac atgggagacc gcgaaggccc tcgaggtgct cccgaacgag
     3241 gtcctcatcg cccagacggg aattatcggc gtgccgttcc cggtcacgcg cgtcaaacgg
     3301 gcgatcccga aactggtcgc gaagctctcc gacacgggcg gtcacggcgc ggcgaaaggg
     3361 attctgacaa cggacctttc caacaaggag atcgcgcttt cgatcgagct cggcgggaag
     3421 aaagtgacga tcgcggcctg cgcgaagggc gcgggtatgg tgcacccgaa tatggcgacg
     3481 atgctttgtt tcatcacgac cgattgcgcg atcacgaaac cgatgctgcg gcgtgcggtg
     3541 cgccacgcgg cggacgacac gttcaacagc atggcgatcg acaacgatat gagcaccaac
     3601 gacatggtgc tttgcctcgc gaacggcctg gccggcaacc caacgatcgc gaaggtcgac
     3661 cgcgactact ggatgttccg tgaggccctc gaagaggtta tgcggtacat cgcctatgaa
     3721 atggtgaagg acggcgaggg cgtgacgcac gtctgcaaac tgcgtctctc gggggcgaaa
     3781 accccgacgg acgccgagcg cgcggcgcgg cagatcgcca actcgatgct tttcaagacc
     3841 atgctcgcgg gttcgaaccc gaactggggg cgcatcgcgg ccgcggttgg cgcgagcggc
     3901 gtggatttca acccgaacca cctgagtatc tcgttcggtg acgccgaagt cgtgcacctc
     3961 gggaagcttc gcgtcctcaa catcccgaag gcgcggcgga tcctccagaa aaaagagtac
     4021 acgatcgatg tcaagatcgg caaaggccgc ggccgggcgg agtttatcac gtccgacctc
     4081 acgacgaagt acgttctcat taacgcggca tattcctaga gaaagggaag agcgtgcagt
     4141 gtagagcgta ggggaaaact ccacgccgaa cacgctgcgc tccacacgaa aaaatgcagg
     4201 aactgatcaa aaaggccggc gtcctcatcg aagcgcttcc gtacattcag aaattccgcg
     4261 gcaaggaagt tctcatcaag tatggcggtg ccgcgctcac gtaccaggaa gtgcgggaca
     4321 tggtgctcca ggatctcgtg ttcatgagct acgtcggtat ccgtccggtc ctgattcacg
     4381 gcggcggcaa gtacatcacc gagaacatga agaaaaaggg catccagagc cagtttatca
     4441 acggactgcg cgtgacggac aaacccgcga tttcggttgt caacgaaacg ctggccgagg
     4501 tcaaccgcgg catcgttcac gacatcgaac ggctcggcgg ttcggcggtc ggtctttccg
     4561 gttacgacgc aaaggtcctg agtgtcgtga agaacacgga acacggggac atcggtttcg
     4621 tcggcgacgt gtcctcggtc aacacggtcc cgatcaagga actgacgggg ttcggaaaga
     4681 ttcccgtgat ctttccggtc ggtaaggggc cggacggcga aatatacaac gtgaacgcgg
     4741 acgaggcggc ggccaacatc gcggccgcca tgcaagtcca gaagttcatg gtgcttacga
     4801 acgtgagggg catcctgcgc gatttcaagg atgaaagcac cctgatcccg tcactgtgcg
     4861 tgggcgacac gcagaggctg atcgaccagc agatcatctc gggaggcatg atcccgaagg
     4921 tgaaggcctg catcgacgcg cttgagggcg gtgtccccaa agcgcacatc atcgacgggc
     4981 gcatcccgca cgcgttgcta ctcgagattt ttaccgatca gggaatcgga acggaaataa
     5041 ctctataggg atcagccatc agcgatcagc tatcagtaaa ggctgaatgc cgagggctga
     5101 gaactgaaca cagggctatg aaaaccacag acgtccagaa actttataac gaatacgtga
     5161 tgcccactta cacgcggctg ccggtctgtc tcgtgaaggg gaaaggatcg cgggtctggg
     5221 acctggaagg caaggagtat cttgatttct tcccgggctg gggcgtgtcg tgcctggggc
     5281 actgccatcc ggccgtgatc aacgcgatca aacaccagtc ccggacgatg ctccacatcc
     5341 cgaacaactt cctcaacatc aagcagtcgg agctcgccag cgagattgtg aaagcctcct
     5401 tcccgggacg cgtattcttc ggcaattccg gcgccgaggc gaacgagggt gccatcaag
//
LOCUS       DAOOBX010000017         5350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_17623_length_5350_cov_2.839282, whole genome shotgun sequence.
ACCESSION   DAOOBX010000017 DAOOBX010000000
VERSION     DAOOBX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17623_length_5350_cov_2.839282"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1639
                     /gene="rimO"
                     /locus_tag="PLY30_00560"
     CDS             305..1639
                     /gene="rimO"
                     /locus_tag="PLY30_00560"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPW64620.1"
                     /translation="MKARKIRIGIVSLGCPKTLVDSEIVLGKLKDPRYALAGNVDESD
                     VVLVNTCGFIRDAKDESIDVILRLIEYRRTGRIRALVVMGCLVQRYAAELAGNFPEVD
                     AFLGSGDYNRITAILRSVGEGERSVILGHPGYLGKAREERIPLTPRYSRYLKISEGCD
                     HTCSFCVIPKIRGKFRSRSIPDVVREACALVRTGAKELILIGQDITKFGCDYARKPLL
                     PELLDALEPIPGLEWIRLLYAYPNSLNARMIAKIAGSGKICHYLDLPLQHISDKILTA
                     MRRGGGRHRTVELIKKLRGEIPDLALRTSFIVGFPGETERDFGELLDFMAWAKFERAG
                     VFRYSREEGSAAAAFPAQVSERVKDLRFHKAMSLQKKVAKNVSRRQTGCELRVLIEGK
                     LAGGNLWKGRSYMDAPEIDPHVIVESSHPLKTGAFYPVMITGAKGYDLVGKI"
     gene            1626..2633
                     /locus_tag="PLY30_00565"
     CDS             1626..2633
                     /locus_tag="PLY30_00565"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPW64621.1"
                     /translation="MEKFKFAVLHPNHLSFFRGIIGVLLPFLILSRSPAAHLAAAALF
                     ILGAVTDYWDGWLARQYQLESKFGKWIDPFMDKILILAPLAAFANIGFFSLWWLVPIF
                     FREILVTFCRTAWLLEGKSFGAEKLGKLKFVFQTGSVLLAFAVFILWDYAGTEAVARW
                     LAPAVVPVLGIAVLLTLLSGFFFLWNQRECFASRHFSRFTLATGVGLLPFAPGTWGSL
                     VGLLVVLLTAWNFYLYAAVLAGLIVVGDVAFRKLEDRSDPDPQFIVIDEAVGIMVTFL
                     LVPLTWITVPLGFLLFRLFDVKKPFPIRKLERIPGYWGIMADDIGAGIYAWFPLFLFF
                     A"
     gene            2645..3865
                     /locus_tag="PLY30_00570"
     CDS             2645..3865
                     /locus_tag="PLY30_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HPW64622.1"
                     /translation="MRPRAAVFSVGNEVLKGSVVNSNAAFLGRELTAAGFEVIAHAVC
                     TDSEESIRFQLGDLTRRADLIVMCGGLGPTPDDVTREGIAAFFNVPLVFFKDQFARIV
                     KLYRRFGKSVPPLVRREALYPANAKPLVNRFGVALGFAVEQSGKVLVALPGVPAELEN
                     MYADVVLPFLKKRFPAAAPSRRLVVRMTGISEPDVMERLGKGLFEDPFDFGIYPSAGE
                     VVLRIYSESPGVHARLKKRIGARLRDFIYSWEERPLGFVIGELLRKKKRTLAVAESCT
                     GGLLAAEIVSQPGSSRFFRGGTIAYHHEIKEKLGVDPALITRHGEVSAAVAGALASSV
                     KDRMKTSYGLGITGIAGPSGGTPKKPVGLVYIGLAGPSGRVNVWQHHFGGDRDQVCRR
                     TVVKALEYLWRAVR"
     gene            3862..4443
                     /gene="thpR"
                     /locus_tag="PLY30_00575"
     CDS             3862..4443
                     /gene="thpR"
                     /locus_tag="PLY30_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPW64623.1"
                     /translation="MSGEVLRLFLALPLSVFRDEIERFLVLLKRNVPGIKWVLPEQVH
                     LTLHFFGDTPSSGLDRLDEALRRVVGDFPVMELRLTGMGGFPDLKRPNVLWAGVEDRE
                     GKLPEFENSVRKGIAGLGYPVETRPFRPHATLGRTKSVPPGHAIHETVSRLPLQLPTA
                     FKPVDRLILYKSELRSSGAVHEIVREFPFNGAA"
     gene            4403..5167
                     /locus_tag="PLY30_00580"
     CDS             4403..5167
                     /locus_tag="PLY30_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HPW64624.1"
                     /translation="MKSSGNSRLTALPRFTPAELHGVYQRLRDRFGHQRWWPADTPFE
                     VMIGAILTQNTAWTNVEKAILNLKRAGRLSFRGINHMTRRQLARLIRPAGYFNIKADR
                     IKHFVRFLGREYGGNFRKMRREPVAVLREKLLRVKGIGPETADSILLYALGKRSFVID
                     AYTRRIFSRHGLMAGDRDYHEWKDLFEKALPPSRAVYNDFHAQIVYLAKHFCRTVPVC
                     GGCPLQGLHRRKKMQNSFNILPLSRVRYNAGKVIHG"
     gene            5160..>5350
                     /locus_tag="PLY30_00585"
     CDS             5160..>5350
                     /locus_tag="PLY30_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64625.1"
                     /translation="MAEKVTKKLGEILVEDGLIRSPQLTEALAHQREHGSLLGQILVE
                     KGFIDEDSLVSTLGKQFRIP"
BASE COUNT          988 a         1496 c         1619 g         1247 t
ORIGIN      
        1 ctccggactg tcgatccgga ggttgcgggt tcgagccccg tcgctcccgc catttcttcc
       61 gtaaaattcg ggagagggtc gaaatgaccg cacaggccgc tgccgtttct tccttctgtc
      121 ccccaaatgg tcgaagtgac tgcgcaggtc gccgccattt ctcctgttaa tcaagcagat
      181 ggccggcatg actgcctcgg acgaagtgtt tttttgtgat agccggggtt atagaagagg
      241 ccgtctatgt agttgaagtt acttcgttga ttcctgagac ccattccctt agaatccccc
      301 ctctatgaaa gcgcgaaaga ttcgcatcgg cattgtgtct ctcggttgtc ccaaaactct
      361 cgtggacagc gagatcgttc tcggcaagct caaggacccc cgctacgcgc ttgcgggaaa
      421 tgtcgatgaa agcgatgtgg tgctcgtcaa tacctgtggt tttattcgtg acgccaagga
      481 cgaatcgatc gacgtgatcc ttcgcctgat tgaataccgg agaacaggca ggattcgtgc
      541 gcttgtcgtg atgggatgcc tggtccagag gtacgcggcc gaactcgccg ggaatttccc
      601 ggaggtggat gcctttctgg gctccggcga ttacaacagg atcactgcga ttctccggtc
      661 ggtcggggaa ggtgagcgtt ccgttatcct gggccatccc ggttatctcg ggaaagcacg
      721 ggaggaacgg attcccctga ccccccggta ttcccgctac ctcaagatct cggaaggttg
      781 cgaccacacc tgctcgttct gtgtgatccc gaagattcgc gggaaattcc ggtcccgttc
      841 catcccggac gtcgttcgtg aggcttgtgc gctcgtccgg acaggcgcga aggaactgat
      901 cctcatcggt caggacatta cgaagttcgg ctgtgattat gcccgcaagc cgctactccc
      961 cgaacttctc gacgcgcttg aaccgattcc cgggctggaa tggatccggc ttctttacgc
     1021 atatccgaat tcgctgaatg cccggatgat cgcgaagatc gcgggctccg ggaagatttg
     1081 ccattacctt gatttaccgc tccagcatat tagtgataag atactcaccg caatgcgccg
     1141 ggggggcggg cggcaccgga cggtcgagtt gatcaagaag ctccgcggcg agatcccgga
     1201 tctcgcgtta cggaccagtt tcatcgtggg atttcccgga gagaccgaac gcgatttcgg
     1261 cgagcttctg gatttcatgg cgtgggcgaa attcgagcga gccggcgttt tcaggtattc
     1321 acgggaggaa ggttcggcgg ccgccgcgtt tcccgcccag gtcagcgaaa gggtgaagga
     1381 tctccgtttt cataaagcga tgtcgctcca gaaaaaagtg gcaaaaaacg tgagccgccg
     1441 ccagacaggg tgcgagttgc gcgtcctgat cgaaggaaaa ctcgcgggcg ggaacctctg
     1501 gaaaggccgt tcctacatgg atgcgccgga gattgatccg cacgtgattg ttgaaagttc
     1561 ccatcccctg aaaacggggg cgttttatcc ggtcatgatc acaggagcca aaggctatga
     1621 tctcgttgga aaaatttaaa ttcgcggtcc ttcatcccaa tcacctcagt ttttttcgcg
     1681 ggatcatcgg tgttttgctt cccttcctga tcctgtcgcg atcgcccgcc gcgcatctcg
     1741 cggcggccgc gctttttatt ctcggtgccg tgaccgatta ctgggatggc tggcttgccc
     1801 gtcaatatca gctcgagtcc aagttcggga aatggatcga tccgttcatg gacaagattc
     1861 tcatcctggc gccgctcgcg gctttcgcga atatcggttt tttttcgctt tggtggctgg
     1921 tgccgatctt tttccgcgag attctcgtga ccttctgccg cacggcctgg cttctggagg
     1981 gaaaatcgtt cggagcggaa aagctcggca aactgaaatt tgtctttcag acgggcagtg
     2041 tccttctcgc gttcgccgtt ttcatcctct gggattacgc ggggacggaa gcggtcgcca
     2101 ggtggctggc ccccgcggtg gtcccggttc tcggcatcgc ggttctcttg acgcttcttt
     2161 cggggttttt tttcctgtgg aaccagcgcg agtgtttcgc atcccggcac ttttcacgtt
     2221 tcacgctcgc gaccggcgtc ggcctgttgc cgttcgcgcc cggaacgtgg ggaagtctcg
     2281 tggggcttct tgtggtgctc ctcacggcct ggaactttta tctctacgcc gccgtcttgg
     2341 ccgggttgat tgttgtgggg gacgttgcct tccgaaaact ggaggaccgg agcgatccgg
     2401 acccgcagtt catcgtcatc gacgaggccg tcgggatcat ggtgaccttt cttctggttc
     2461 cgttgacctg gattaccgtg ccgctcgggt ttctgctctt ccgcctgttt gacgtcaaaa
     2521 agccattccc gatacggaaa ctcgaacgga tccccggcta ctgggggatc atggccgatg
     2581 atatcggcgc gggcatttac gcctggttcc ctctcttcct tttcttcgcc tgagggtgtt
     2641 tgcgatgcgg cctcgcgcag cggttttttc tgtcggtaac gaagtcctga aagggagcgt
     2701 ggtcaactcg aatgccgcgt tcctgggacg ggagctgacc gccgccggtt tcgaggtcat
     2761 cgcgcatgcg gtctgcactg attccgaaga atccatccgg ttccagctcg gggatctgac
     2821 ccggcgcgcg gacctgatcg tcatgtgcgg cggtctcggg ccgaccccgg acgatgtgac
     2881 ccgggagggc atcgcggcgt ttttcaatgt cccgcttgtt ttcttcaagg accagtttgc
     2941 gaggatcgtc aaactttacc ggcgtttcgg aaagtccgtg ccgccgcttg tccggcgcga
     3001 ggccctgtat ccggcgaacg caaagccgct cgtgaaccgt ttcggtgtgg cgctcgggtt
     3061 cgccgtggaa cagagcggaa aagtgctcgt ggcgcttccg ggcgtcccgg ctgaactgga
     3121 gaacatgtat gccgacgtcg tccttccttt cctgaaaaaa cgattcccgg cggccgcgcc
     3181 gtcccgacgt cttgtcgtcc ggatgaccgg gatctccgaa ccggatgtga tggaacggct
     3241 ggggaagggc ctcttcgagg atccctttga tttcgggatc tatccctcgg ccggagaggt
     3301 ggtactccgg atttattccg aatcacccgg cgtgcacgcg aggttgaaaa agaggatcgg
     3361 ggcacggctc cgcgatttca tctactcgtg ggaggagcgg ccgctcggct tcgtgatcgg
     3421 cgaactcttg aggaaaaaga agaggacgct tgctgtcgcc gagagttgca cgggaggcct
     3481 tcttgcggcc gagatcgtgt cccagccggg atcgagtcgt ttcttcaggg gagggaccat
     3541 cgcgtatcat cacgagatca aagaaaagct cggtgttgat ccggcgctga tcacgagaca
     3601 cggcgaggtc tctgccgcgg tggccggggc gctggcttct tccgttaagg atcgtatgaa
     3661 aacgagctac ggtctgggga ttacggggat cgcgggaccc tccggcggga ctccgaaaaa
     3721 acccgtcggc ctggtgtata tcggtctggc cggtccgtcg ggccgggtga atgtgtggca
     3781 gcatcatttt gggggcgacc gggaccaggt ttgccgccgg accgttgtga aagcccttga
     3841 atatctgtgg cgtgcggtcc gatgagcggg gaggttcttc gccttttcct cgccctgccg
     3901 ctgtcagttt ttcgagatga aatcgaaagg ttcctcgtcc tcctcaagcg caatgtcccc
     3961 ggcatcaagt gggtgctccc ggaacaggtc catctgacgc tccatttttt cggcgatacg
     4021 ccttcttccg ggctcgatcg cctcgatgag gcgcttcgcc gggtcgttgg ggattttccg
     4081 gtgatggaat tgcggctgac cggcatgggg ggattcccgg acctgaaacg cccgaacgtg
     4141 ctttgggccg gcgtggagga cagggaagga aaacttcccg agtttgaaaa cagcgttcgg
     4201 aaaggaatcg cggggctcgg ctatcctgtt gaaacgcggc cgttcaggcc ccacgcgacc
     4261 ctgggacgca cgaagagtgt cccgccggga catgcgatcc atgaaacggt ttcgaggctt
     4321 cctcttcagc tcccgacagc cttcaaaccg gttgaccgtc ttatccttta taaaagtgaa
     4381 ctgcgttcct caggagccgt ccatgaaatc gtcagggaat tcccgtttaa cggcgctgcc
     4441 taggttcacg cccgcggaac tccacggcgt ctatcagcgg ctgagagacc gtttcggaca
     4501 tcagcgctgg tggcccgctg atacgccctt tgaggtcatg atcggcgcga tcctgaccca
     4561 gaacaccgcg tggacgaacg ttgaaaaagc gatccttaac ctgaagcggg ccggccggct
     4621 ttcctttcgc ggcatcaatc acatgacccg tcggcagctg gcccggttga tccgccccgc
     4681 cgggtacttc aatataaagg ccgatcgcat caaacatttc gtccgttttc tcggtcggga
     4741 atacggcggg aacttcagga agatgcgccg ggaaccggtg gccgtgcttc gcgagaaact
     4801 tctccgtgtg aaaggaatcg gtcccgagac ggccgactcg atcctcctgt atgctctcgg
     4861 caaacgttcg ttcgtcatcg acgcttatac acggcgcatt ttttcgcggc acgggctcat
     4921 ggccggggac cgggactatc acgaatggaa agacctgttc gaaaaggcgc ttccgccgag
     4981 tcgcgcggtg tataatgatt tccacgccca gattgtgtat ctggccaagc atttttgccg
     5041 gacggtaccg gtttgcggag gatgtccgct tcaaggtctg cacaggcgca aaaagatgca
     5101 aaactctttc aatatcctgc cgttatctag ggtgcggtat aacgcgggga aagtcatcca
     5161 tggctgaaaa agtcacaaaa aaactgggag agattcttgt cgaagacggt cttatcaggt
     5221 cgcctcagct tacggaggcg ctcgctcatc agcgtgagca tggcagcctg ctcgggcaga
     5281 ttctcgtgga aaaaggtttc atcgacgagg acagtctcgt aagcacgctc gggaaacagt
     5341 tccggattcc
//
LOCUS       DAOOBX010000018         5302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_17817_length_5302_cov_4.227368, whole genome shotgun sequence.
ACCESSION   DAOOBX010000018 DAOOBX010000000
VERSION     DAOOBX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17817_length_5302_cov_4.227368"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5302
                     /locus_tag="PLY30_00590"
     CDS             <1..>5302
                     /locus_tag="PLY30_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64626.1"
                     /translation="DVSCTWQSADRKTSYSLSESLVKLKQTDGRFTEGREYSFSAQVR
                     VSAALMEARKTELFSRLSAAEQEQLLVALGSVDSDGFSMIVTGTDVSYTWQSPDRTRS
                     YNLNQQKVYFTDGSEGWDVSFSTQYRVSYELFLTENPASALEDIKEGPLAEADQLAAY
                     NAALPHARSSLEGISLSVSGNSSTWSWRDGDRNYNYSLSRDRSYFSNDAGADAEYTVK
                     TTMTAQYYAGRALMEAFLESLPVREREGFQNGLNDRSGNYSVTAIGGNETYGWKSADQ
                     KTDYSVSKTLALLADGTRAPAFSVSYEYHFSDARRSSSAEMKNLLEADVPPVLMTAAE
                     KGALRAVLEDPSLKITRTRQGAETTYTISTSSHPGHAYSLTKNSALPDAKWDYSVRTR
                     LTVAELKSAMSGILDPEMLAQFKAVTGYNEATGELSAMNDSIEVYRSASGYNNALSYS
                     WDMPGTGFSYSVNIDTRVPTGTSGGYMTVSTFTRQAQVLRSVIDAQIGLIKDPAQRPV
                     DLPPADERTVYTWATSGISDSFSWSSGQGVVSHSISLDKSFKKADGSSAPVTVANYSR
                     TTRVRLSDVTAMAESVLDPNERAQLLSALYKDPDAQAREINDGLEKASFALTETGNSR
                     TYTWSSPDGNVTYNLGVETKVPSGIAGGFTSVASFSVQTRVSVEVLRGLIALVRDSSE
                     KSLLEAALSSANSELGYTSFSMTVAGKQTSYSWGSRDGNTSHTVSVERDPDAASGVKR
                     ASYSRSERVARSEVGSTVSTIYDAGQCSLLTADFAATSAMEDYLSFYRQTTGNRISYT
                     WSAPDGNATHTVTTDSSSAEPRPGYVRQIRVSREQMIKDLISEGTITGTITETGTGEF
                     EVTGEAKTLYDAILTNIQDAFAAVGIATGWCAAFFYKSFSGLDLTDISYSWSLAGVSA
                     LGLAVSHSLTVLLGGGYRWDVQQTVSRTDGTVEAFGGFGTLTGAQWDNQDVTHTTSYR
                     SSPGGVVRDTESYTITSADGLETRTASRDHARAPFTRTITVQQRGAEYEGKVGDWTVT
                     TSNLGVKIDETTGQITEGFVRIEGRTETVAQMAVRELAPTGGQRETGVTYIINAGIDA
                     VSVDDRGFVVFNETGSHYERSMTIEERDPRTRQRLFRTVQGQEGCWTRTLSNLGAGFI
                     EVTTGSADLTKYSLSMDKYTGGRQVTYFISGYDDASFTTGEFDLSTQTRDWGIHGGDA
                     SAVPPSEGKEGVWVDAVSDYGENFSVLDSTSFSRSLVEIDKQGLTTGVTYMIDEYGRD
                     FEEVDTLKYSRSRVEDTLRDQYGQPCQTAGGKCGLWTRVSSNLTAAFDEDSGSKFSRQ
                     LTDSADEDITYTVSGYDNTFTTPPAVAISPVTGTTYDPAAVTTLPGSPVIVYDTTTMG
                     GADNATVTVTRTAAGFSYSYTLADNDDAVSAAIRWASFNPETGLADGPGTALGASVVL
                     ALSGPDGSVAHVSIVDIKGRSAVFDVTLAGELRNYELQLSGENIPEGFDREHIAGIYF
                     PADKQSMGASGTLTIQAGGLNDSVPPAPVDGFWHRYVRLEYSVTEKEHNAVYGNAVPE
                     IRGNGIRVSSRLRSDFTDDEGRSEYTRQYEERGANGLKTGVTYSISGYLHDFSAAPRG
                     RELEYSLTIKKAGTPWVQSITNIGADFVTEKDSTRYSMHCSEGVEACGVTTGMDLDLG
                     PGDEVTFTIANFSKDFDPGTGEDLETSIQVKESGVHFGVWADWTRTYSNLAVRWGEDG
                     KPENFSAIEGETEYSLTREVKDYSYLGKRGDWNYVYGGLSS"
BASE COUNT         1112 a         1478 c         1701 g         1011 t
ORIGIN      
        1 aggacgtgag ctgcacgtgg cagtccgcgg accgcaagac gagttattct ctgtccgaaa
       61 gtctcgtgaa gttaaaacag accgacggca ggttcaccga gggccgcgag tatagctttt
      121 cggcgcaggt gagagtgtcc gcggctttga tggaggcgcg gaagaccgag ctcttttcac
      181 ggttgagcgc cgccgaacag gaacagcttc tcgttgctct cgggtcggtt gattctgacg
      241 gattttcgat gatagtgaca ggaacggacg tgagctatac gtggcagtct ccggacagga
      301 cccgatccta taatctgaat cagcaaaaag tttattttac tgacggctcc gaaggctggg
      361 acgtgtcttt ttcgacgcag taccgggtaa gctatgaact ctttctgacc gagaatcccg
      421 cgtccgctct tgaagacatc aaggaaggtc cgctcgcgga agccgaccag ctggcggctt
      481 ataacgcggc gctgccgcac gcccgttcgt ccctggaagg gatcagcctg agcgtttcgg
      541 ggaactcctc tacctggtcc tggcgcgacg gtgacaggaa ctacaattac tcgctcagcc
      601 gtgaccggtc gtatttttcc aatgatgccg gcgccgatgc cgagtatacg gtcaagacga
      661 cgatgacggc gcagtattat gccggcaggg cccttatgga ggcgtttctg gagagcctgc
      721 cggtccggga gcgggagggc tttcagaacg gactcaatga ccgaagcggt aattattcgg
      781 tcacggcgat cggcgggaat gaaacgtacg gctggaaatc cgcggatcaa aagaccgatt
      841 acagtgtgtc gaagacgctc gcgctgcttg cggacgggac ccgggcgccc gctttctctg
      901 tatcttatga atatcatttt tccgacgcgc gccggtcttc gtccgcggaa atgaaaaatt
      961 tgctggaggc ggatgttccg ccggtattga tgacggccgc ggaaaagggt gccttgcgtg
     1021 ctgttctgga agatccgtcg cttaagatca cccggacgcg ccagggagcg gaaacgactt
     1081 atacgatcag cacgagttca catccgggac acgcctacag cctgacaaaa aattcagcgt
     1141 tgcccgatgc caaatgggac tattcggtca ggacgcggct gacggtagcg gaactcaaga
     1201 gcgccatgtc cgggattctg gatccagaaa tgctcgctca gttcaaggcc gtgaccggtt
     1261 ataacgaggc gaccggggaa ctgagcgcga tgaatgactc tatcgaggtc taccggtcgg
     1321 cgagcggata caacaacgct ctgtcctaca gctgggatat gcccgggacc gggttcagtt
     1381 attccgtgaa tatcgacacg cgtgttccga cggggacgtc cggaggctat atgacggtgt
     1441 cgacgttcac ccgccaggcg caggtcctga ggtccgtgat cgacgcgcag atcggtctga
     1501 tcaaggaccc ggcacaaagg cccgtagacc tgcctcccgc cgatgagcgg acggtttaca
     1561 catgggcgac gagcggtatt tcggacagtt tctcctggtc ttccgggcaa ggggtcgtgt
     1621 cgcattcgat ctccctcgac aaatccttca aaaaagccga cggatcatcc gcgcccgtga
     1681 cggtcgcgaa ttactccagg accacccggg tccggctgtc ggatgtcacg gcgatggctg
     1741 aaagcgtact ggatccgaac gagcgcgccc agctcctgag cgcgctctac aaggatcctg
     1801 acgcgcaggc gcgggagatc aatgacgggc ttgaaaaagc gagcttcgcg ctgaccgaaa
     1861 ccggaaattc caggacctat acctggagct cgccggacgg taacgtcaca tataacctcg
     1921 gcgtcgaaac aaaggtgccc tcgggtatcg cgggcggttt cacgagtgtg gcttccttca
     1981 gcgttcagac gagggtcagc gtcgaggtct tgagaggtct gatcgcgctt gtcagggact
     2041 cgtcggagaa gtccctgctt gaggccgctc tctcttccgc caacagcgaa ctcggctaca
     2101 cgtcgttctc gatgacggtc gcgggaaaac agacctcgta ttcatggggt tcgcgcgacg
     2161 ggaacacgag ccacacggtg tcggtcgaaa gagaccccga cgcggcctcc ggtgtgaaac
     2221 gcgcgtcgta cagccgctcg gagcgtgtcg cgcgcagcga ggtcggctcg acggtttcaa
     2281 cgatctacga cgccgggcaa tgttcgctgc tcaccgcgga tttcgcggcc acaagcgcga
     2341 tggaggatta tctgtctttt taccggcaga cgaccggaaa caggatttcc tacacgtggt
     2401 ccgctccgga cggtaacgcc acgcacaccg tcaccaccga ttcgtcgagc gccgagccgc
     2461 gaccgggcta cgtcagacag atccgcgtga gccgtgagca gatgatcaag gaccttatca
     2521 gcgaagggac tatcaccggg acgatcacgg agaccgggac gggcgaattc gaagtgaccg
     2581 gcgaggccaa aacgctctac gatgcgatcc tgacaaatat ccaagatgcc tttgccgcgg
     2641 tcggcatcgc cacgggctgg tgtgcggcct tcttctataa gtctttttcg gggctggatt
     2701 tgacggatat ctcctactcg tggtcgctgg ccggcgtttc agcgctcggt ctggccgttt
     2761 cgcactccct gaccgtgctc ctcggcggcg gttaccgttg ggacgtgcaa cagacggtat
     2821 cgaggacgga cggcacggtt gaagcttttg gcgggttcgg cacgctgacc ggagcgcagt
     2881 gggataacca ggacgtgacg cacaccactt cgtaccgctc gtcgcccggc ggagttgtcc
     2941 gcgacacgga gagttacacg atcacttccg cggacggcct tgaaacccgg acggccagcc
     3001 gtgaccacgc ccgggcgccg ttcacccgaa cgatcaccgt ccagcagcgc ggggcggagt
     3061 atgaaggcaa ggtcggcgat tggaccgtga cgacgagcaa cctcggggtc aagatcgatg
     3121 agaccaccgg tcagatcacc gaggggtttg tccggatcga aggtaggacc gagaccgtcg
     3181 cgcagatggc cgtgcgtgaa ttagcgccga ccggcgggca gagggagacc ggtgtgacat
     3241 atatcatcaa cgcggggatc gatgcggttt ccgttgatga caggggattt gtcgtgttca
     3301 acgaaaccgg ctcccactac gaacgctcca tgacgatcga ggagcgcgat ccccggaccc
     3361 gccagaggct cttccggacg gtgcagggac aggagggctg ctggacaagg acgctgagca
     3421 acctcggcgc agggttcatc gaagtgacca ccggcagcgc cgacctgacg aaatacagtc
     3481 tgtcgatgga taaatacacg ggtggcaggc aagtgaccta cttcatcagc ggctatgatg
     3541 acgcctcttt cacgaccggg gaattcgatc tctcgacgca gacgcgcgat tgggggatcc
     3601 acggcgggga cgcgtcggcg gttcctccgt ccgagggcaa ggaaggcgtc tgggtcgatg
     3661 cggtcagcga ctacggcgaa aacttttcgg tcctcgactc cacttcgttc agccggagcc
     3721 ttgtcgagat cgacaagcag gggctcacga ccggcgtaac ctacatgatc gacgagtacg
     3781 gcagggattt tgaagaagtg gataccctca aatattccag gtcgcgcgtt gaggacactc
     3841 tcagggacca gtacggccag ccatgtcaaa ctgccggcgg gaaatgcggg ttatggaccc
     3901 gggtctccag caaccttacg gcggcttttg acgaagattc ggggagcaag ttcagccgtc
     3961 agcttacgga ctccgcggat gaggacatca cttacacggt cagcggttac gataacacct
     4021 tcacaacgcc tcccgctgtg gcgatcagtc cggtcacggg aaccacgtat gatcctgccg
     4081 cggtgacgac actgcccggc tcgccggtca tcgtttatga cacgaccacg atgggggggg
     4141 cggacaacgc taccgtgacg gtcacccgga cggccgcagg tttttcttac agctacaccc
     4201 tggctgacaa tgatgacgcc gtcagcgccg cgatccgctg ggcgtcgttc aatccggaaa
     4261 ccggtctcgc ggacggaccg ggcacggcgc tgggtgcgag cgtggtcctt gcgctgagcg
     4321 gcccggacgg cagtgtggcg catgtcagta tcgtggatat caaggggcgc tcggcggtct
     4381 ttgatgtgac cctcgcgggc gagcttcgga actatgaact gcagctgagc ggagaaaata
     4441 tcccggaggg tttcgaccgc gaacatatcg ccggtattta ttttcccgcc gacaagcaaa
     4501 gcatgggagc ttcgggaaca ctgacgatcc aggctggcgg cctgaatgat tcggttcctc
     4561 ccgcgcccgt tgacggattt tggcaccggt acgtccgtct tgagtattcc gtgacggaaa
     4621 aggagcacaa cgcggtatac gggaacgccg tgcctgagat tcgcgggaac ggaatccggg
     4681 tctcgagccg tttgcggagc gattttacgg acgatgaagg gcgttcggag tacacgcgtc
     4741 agtacgagga gcgcggtgcg aacgggttga agaccggcgt cacctacagt atttccggat
     4801 acctgcatga tttctccgcc gctccgcggg gccgggagct ggaatattct ttgacgatca
     4861 aaaaggcggg gacgccctgg gttcagagca tcacgaacat cggagctgat ttcgttacgg
     4921 aaaaagacag caccagatac agcatgcact gcagcgaggg ggtggaggcc tgcggggtga
     4981 cgacggggat ggacctggac ctcgggcccg gggacgaagt cacgttcacc atcgccaatt
     5041 tctcgaaaga ttttgatccc gggacggggg aggacctcga gacctcaatc caggtgaagg
     5101 aaagcggcgt tcatttcggg gtctgggcgg actggacgag gacctactcc aatctggcgg
     5161 tgcgctgggg tgaagacggg aagcctgaga atttttccgc gatcgaggga gagacggagt
     5221 acagcctgac gcgggaagtg aaggattatt cgtacctcgg aaagcggggc gactggaatt
     5281 atgtgtacgg cggtctgtcc tc
//
LOCUS       DAOOBX010000019         5186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_18297_length_5186_cov_3.289027, whole genome shotgun sequence.
ACCESSION   DAOOBX010000019 DAOOBX010000000
VERSION     DAOOBX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18297_length_5186_cov_3.289027"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="PLY30_00595"
     CDS             complement(<1..136)
                     /locus_tag="PLY30_00595"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HPW64627.1"
                     /translation="MNRILIGAQWGDEGKGKVIDILAAKSDVVVRYQGGPNAGHTIKV
                     H"
     gene            complement(251..1846)
                     /gene="serA"
                     /locus_tag="PLY30_00600"
     CDS             complement(251..1846)
                     /gene="serA"
                     /locus_tag="PLY30_00600"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPW64628.1"
                     /translation="MKMMKVLVSDSLGQGGMAVLRREKNLQVDEKVGLKPEELKKIIG
                     DYDAILVRSATKVTKEIIEAGTKLRVIGRAGVGVDNVDLEAATKRGIIVMNTPEGNTI
                     STAELSFSMLMAVARNIPQADTHVKKGEWKRNKFKGSELNGKTLGIVGFGRIGREVAK
                     RARAFNMKIMVFDPFISKESVKEWPVEFVDIPTLLRNSDFLTFHTPLTPETKNLLDKE
                     TFKLCKQGMRIVNCARGGIVDEAALAEAIQTGQIAGAAFDVFTSEPPPADHPLLKLPQ
                     FICTPHLGAVTDEAQENVALDVAVQVIDALNDRAIRNAVNMPNLDPDTLKGLKPWLVL
                     AERIGALYPQLFDGSIKKVEIRYGGDPTRFKTASLTVAILKGLLTPICGDTVNFVNAP
                     AIARERGITISEGVSDETVDFSSFIDVRVAQGKEVHRIMGTLFNQQPRIVRINDFLID
                     LVPEGWVIFVRNEDLPGVVGHIGTILGKNGINIAEMSLGRLGKGKKAYAMTAINTDSE
                     VPMKVVSRFKTLKSVMDVKVVKL"
     gene            complement(1894..3009)
                     /locus_tag="PLY30_00605"
     CDS             complement(1894..3009)
                     /locus_tag="PLY30_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPW64629.1"
                     /translation="MINPTILLTPGPTPVPEAVRKLMAEPIFHHRTPRYRKIFGDVSE
                     RLKKVFMTRNPVYTFAGSGSLAMEAAIVNFHSTGDKILIGENGKFAERFTAIARAFGL
                     NPIVIKMPLGEAVPPAMIEEALKKDPDIKAVCVQLCETSTAVLNDIKAIGAVVAKTNA
                     LLIVDAISGLGADRIETDNWNVDLVIAGSQKALMLPPGLAFLSVSEKARARMATAKLP
                     RYYYDLKYYEKSLRDSDTPWTPALTLVIGLQKSLEMIEAEGLENVFKRCSENAQFTRD
                     TLRKMGLNIFSKAPSSTVTAACMPEGVDGEKLTQYMRDEKGIAMAGGQGDQMKGKVVR
                     ICHMGAITRKDLETGLKVLDESLKEKKSSSGSCCCCK"
     gene            complement(3011..3607)
                     /gene="clpP"
                     /locus_tag="PLY30_00610"
     CDS             complement(3011..3607)
                     /gene="clpP"
                     /locus_tag="PLY30_00610"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPW64630.1"
                     /translation="MSYLVPMVIETTGRGERAYDIYSRLLKDRIVFIGTPIDDLIANM
                     IIAQILFLNMEDPGKDINVYINSPGGSVTAGLAIYDTMQFVKCDVATFCLGQAASMGA
                     FLLAAGTRGKRYALPNARVMIHQPWGGAQGTASDIHIQAREILKLKDQLNTLLAHHTG
                     KPREQIEKDTDRDYFMSAEESRSYGIVDQVITSKTEIK"
     gene            complement(3695..5035)
                     /gene="tig"
                     /locus_tag="PLY30_00615"
     CDS             complement(3695..5035)
                     /gene="tig"
                     /locus_tag="PLY30_00615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPW64631.1"
                     /translation="MDFFKKKKNDPNQDSTATEPTEASPFRIKLQDGKVCEKILTIEV
                     AKDRIAEEYRNYYAAIAPKAKVPGFRPGKAPRHVLEMHYEKNANDAVLEQLLSESLPY
                     AIREKGLSPLAAPELHDVQFTREKLSFKAHVEIRPKVKLSRVEGLNAKREKAEVKPDE
                     LEKAVENLRERHCRYKAVERPAAMGDFMVMDYVCVIEGKELEKRQDDWVELKASEYLK
                     GFSEQLVGLKAGDAKDIQLNMPENMQNRNMAGKPATFKVTVKEVKEKFLPAVDDELAK
                     QAGDYKDLADLREKLRQDLEKRKADEKEAEFERALFDELLKHNKIDIPQGLLARRTGN
                     LVEQSKQRFIYQGGTAELFDQEKDKVCKEFEAEAKRQIHLAFLLDEIAGTKQIKVEEA
                     DLKQKYNDLAARYRQSAETIEKYYQEHKEALESLLDQVRNEKVIEYIKANAKQS"
BASE COUNT          974 a         1533 c         1416 g         1263 t
ORIGIN      
        1 cgtggacctt gatcgtatgc cccgcgttcg gtccgccctg ataacggacc accacatcac
       61 ttttcgcggc aaggatatca ataaccttgc cttttccctc atcgccccac tgggcaccga
      121 tcagaatcct gttcacccgt caaaccctca cgttaaaaga tgcgcatatg ccttcagaca
      181 aaattcaacc cccgaagggg aatcccggca gaacgggatt ctaacccccg gggggttgaa
      241 taagatcaaa tcacaatttt acgactttca cgtccatgac ggacttcagc gtcttgaaac
      301 gcgacacgac cttcatcggc acttcgctat cggtgttgat tgccgtcatc gcgtaggcct
      361 tcttgccctt gccgaggcga ccgagagaca tttcggcaat gttgatcccg ttcttcccga
      421 ggatcgttcc gatgtgcccc acgacgccgg gcaggtcctc gttcctcacg aaaatgaccc
      481 agccttccgg cacaagatcg atgaggaaat cgttgatcct gacaatacgc ggctgctggt
      541 tgaaaagggt tcccatgatc cggtggactt cctttccctg ggccacccgg acatcgatga
      601 aactcgaaaa atccaccgtc tcgtcgctga cgccttccga gatcgtgatg ccgcgttccc
      661 tggctatcgc gggcgcgttc acaaagttca cggtgtcacc gcagatcggg gtcaggagac
      721 ctttgaggat tgcgaccgtg agcgacgcgg tcttgaaacg cgtcgggtcg ccgccgtagc
      781 gaatctcgac cttcttgata ctgccgtcaa aaagctgcgg atagagcgcg ccgatccgtt
      841 ccgcgagcac gagccagggt ttcaggccct tgagcgtgtc ggggtcaagg ttcggcatgt
      901 tcacggcgtt cctgatcgcg cggtcgttca gcgcgtcgat cacctggacc gcgacatcaa
      961 gggcgacatt ctcctgggcc tcgtcggtca cggcgccgag gtgcggcgtg cagatgaact
     1021 gcgggagttt gagcagcggg tgatccgccg gaggcggctc gctcgtaaaa acgtcgaacg
     1081 cggcgccggc gatctggccg gtctggatgg cctcggcgag cgcggcctcg tccacgatcc
     1141 cgccccgggc gcagttaaca atcctcatcc cctgcttgca gagcttgaag gtctccttgt
     1201 caagaaggtt cttcgtctcg ggggtgagcg gtgtgtggaa cgtgagaaaa tccgaattcc
     1261 tgaggagagt cggaatatcc acaaactcga ccggccattc cttgacggac tctttcgaga
     1321 taaacggatc gaacaccatg atcttcatgt tgaaggcgcg cgcgcgtttc gcgacctcgc
     1381 ggccgatacg gccgaagccg acaatgccga gcgttttccc gtttagttcg ctgcccttga
     1441 acttgttccg cttccactcc ccttttttga catgcgtgtc ggcctgcggg atgttgcggg
     1501 cgaccgccat cagcatcgag aaactgagtt ctgccgtcga gatcgtgttc ccttcgggcg
     1561 tgttcatgac gatgatcccg cgcttcgtcg cggcttcaag gtccacgttg tccacgccga
     1621 cgccggcgcg gccgatcacg cggagcttcg tgcccgcttc gatgatctct ttcgtgacct
     1681 tggtcgcgct gcggaccaga atcgcgtcgt aatcgccgat gatcttttta agctcttcgg
     1741 gtttgagacc gaccttttcg tcgacctgga ggttcttctc ccggcggagg acggccatgc
     1801 ctccttgtcc cagcgaatcc gatacgagca ccttcatcat cttcattttt cagtctcctc
     1861 ttggaatatt cccgttaaac cgcactcaaa aagttatttg cagcagcaac aggaaccgga
     1921 tgaggacttc ttctctttca gggattcatc gaggaccttg agaccggtct cgagatcttt
     1981 tctcgtaatg gcgcccatat ggcagatccg gacgaccttc cccttcatct gatcgccctg
     2041 cccgccggcc atcgcgatac ctttttcgtc gcgcatgtac tgcgtgagct tttcgccgtc
     2101 cacgccttcc ggcatgcagg cggcggtcac ggtcgaagaa ggcgctttcg agaagatatt
     2161 gagccccatc ttccggagcg tgtcacgcgt gaattgcgcg ttctccgaac agcgtttgaa
     2221 aacgttctcg agcccttccg cttcgatcat ctcgagcgac ttctggagtc cgatcacgag
     2281 cgtgagcgcg ggcgtccacg gcgtgtcgct gtccctgagg gacttttcat agtacttcag
     2341 atcatagtag taacgcggca gtttggcggt cgccatgcgc gccctcgcct tttcgctgac
     2401 gctcaggaac gcaagccccg gaggcagcat gagcgccttc tgggaaccgg cgatcacgag
     2461 gtcgacgttc cagttatcgg tctcgatacg gtcggcgccg agcccggaaa tggcgtccac
     2521 gatcagaagc gcgttcgttt tcgcgacgac cgctccgatc gccttgatgt cgttcaggac
     2581 cgccgtcgaa gtctcgcaaa gctgaacaca gaccgccttg atgtccgggt ccttcttcag
     2641 cgcttcctcg atcatcgccg gaggcacagc ttccccgagc ggcattttga tcacgatcgg
     2701 gttcaggccg aacgccctgg cgatcgcggt gaaacgttcc gcgaattttc cgttctcgcc
     2761 aatgagaatc ttgtcccccg tcgaatggaa attcacgatc gcggcttcca tcgcgagcga
     2821 tcccgacccg gcgaacgtgt agacgggatt ccgggtcatg aacaccttct tcaaccgctc
     2881 ggagacgtcc ccgaaaatct tgcggtagcg gggcgtgcgg tggtggaaga tgggttccgc
     2941 catcaacttg cggacggctt cggggaccgg cgtggggccc ggggtcaaaa ggatcgtggg
     3001 attaatcatt ttattttatc tccgttttgg aagtaataac ctggtcaacg atgccgtaac
     3061 tcctggactc ttcggcggac atgaaataat cgcggtccgt gtccttttcg atctgctctc
     3121 tgggtttacc ggtatggtgg gccaaaagag tgttcagctg gtcctttaat ttcaggatct
     3181 ccctggcctg gatgtggatg tcggacgccg tgccctgcgc gccgccccag ggctggtgga
     3241 tcatgacacg cgcattcggc agcgcgtaac gcttgcccct ggttccggcg gccagaagga
     3301 acgcccccat gctcgccgcc tgcccgaggc agaacgtcgc gacgtcgcat ttgacaaact
     3361 gcatcgtatc gtagatcgcg agacccgccg tcacggagcc gcccggtgaa ttgatgtaga
     3421 cattgatgtc tttgccggga tcttccatat tcaggaagag aatctgcgcg ataatcatgt
     3481 tggcgatcag gtcgtcgatc ggcgtgccga taaaaacgat ccggtccttc aacagccggg
     3541 agtagatatc ataggccctt tcgcctcggc cggtcgtttc aatgaccatc ggaacgagat
     3601 aactcataat tttcaacctt cctttcctgg gcgtggagcg cggagcggac agaaaaaccc
     3661 ggtttcccat acgctgaacg ctacgcgctg aacgttatga ctgtttggcg ttcgccttga
     3721 tgtattcgat gaccttctcg ttgcggacct gatcgagcag ggattctagc gcttccttgt
     3781 gctcttgata atacttttct atggtctcgg ccgactggcg gtaacgggcg gcaagatcgt
     3841 tgtatttttg cttgagatcg gcttcttcaa ccttgatctg tttcgtcccg gcgatctcat
     3901 caagcaaaaa ggcgagatgg atctgccgtt tcgcttccgc ttcgaactcc ttacacacct
     3961 tgtctttttc ctggtcgaag agttcggcgg tcccgccctg atagatgaac cgctgcttcg
     4021 attgttcgac cagattcccg gtcctccggg ccaaaagccc ctggggaata tcgatcttgt
     4081 tatgtttcag caactcgtca aaaagggccc tttcaaattc cgcttctttc tcgtccgcct
     4141 tgcgcttctc gaggtcctgc cgcagtttct cacgaagatc ggcaaggtcc ttgtaatccc
     4201 ccgcctgttt ggcgagctca tcatcgaccg ccggaagaaa cttctccttc acttctttca
     4261 cggtcacctt gaaggtcgcg ggtttgccgg ccatgttcct gttctgcata ttctccggca
     4321 tattcagctg aatatccttt gcgtcgccgg ccttcaggcc gacaagctgt tcggaaaatc
     4381 ccttgaggta ttcggatgcc ttcagctcga cccaatcatc ctggcgcttc tcaagctcct
     4441 tgccttcgat gacgcagacg taatccatga ccatgaaatc gcccatcgct gcggggcgtt
     4501 cgacggcttt gtaccggcaa tgccgttcgc ggagattctc aacggctttt tcgagctcgt
     4561 cgggcttcac ttccgccttt tcccttttcg cgttaagccc ttcgaccctg gaaagcttca
     4621 ccttgggacg aatctccacg tgagccttga aactcagttt ttcgcgggtg aactgcacat
     4681 catgaagctc cggtgcggca agcgggctca gacctttctc gcggatggca tacgggagag
     4741 attccgaaag gagctgttcg agcaccgcgt cattggcgtt cttctcataa tgcatctcga
     4801 gcacatggcg cggcgccttg ccgggacgga acccggggac tttggccttg ggtgcgatcg
     4861 cggcgtaata attccggtac tcttcggcga tccgatcctt cgcgacctcg atcgtgagga
     4921 tcttttcgca gactttcccg tcctggagtt ttatcctgaa gggggacgct tcggtgggtt
     4981 ccgttgcggt gctatcctga ttcgggtcgt ttttcttctt cttgaaaaaa tccattctga
     5041 ataccattcc ttccaggttg tcagatcaaa tccggtgttc ctgtccggca tggagagaaa
     5101 cgcggagaat tgatcttgtc tctccaaaaa aatggagcga gtggagggaa tcgaaccctc
     5161 gtagctgcct tggcaaggca gtgcat
//
LOCUS       DAOOBX010000020         5143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_18468_length_5143_cov_3.109866, whole genome shotgun sequence.
ACCESSION   DAOOBX010000020 DAOOBX010000000
VERSION     DAOOBX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18468_length_5143_cov_3.109866"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="PLY30_00620"
     CDS             <1..1413
                     /locus_tag="PLY30_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW64632.1"
                     /translation="ADLVAVSAFNVQYRRAIEIAREFRSRGRKVAFGGPYCSLFPEAF
                     EGKADHRIVGEAEDIWPLFLRDFEEGKALELYVAPKASVDIRKSPVPRFDLIKGDRYN
                     MFSLQTTRGCPYNCEFCDIIIMDGRKPRTKTVQQVLAEVDHCVQQGAHYIVFGDANFI
                     GNIGFARELLTALADYSKKNGYPIEFSCELTINVAHHPDLLELLQEANFYAVFVGIES
                     PRKQSLLEAGKYQNTREDIIEDIKRIQSYHISVVAGMIVGFDSDDKGIFKETYDFLQE
                     LGIAFTTCGTLMALPHTPLLKRLKAEGRLLDLEWTGMNGHGASDCNFIPKLMTLDELR
                     KGYNWLSRCLYRYDSYADRLVATLNRFRNRNKEHKRASLDTKFLSVLVKVFAYYTLTF
                     DLKRMSFFYGTMWRVAVGGPFSVGKWLEFFRWIATHRAFRQYVMETQGDPEEMDPNSP
                     PFDVECAPVTDDSPREEVLV"
     gene            complement(1887..2078)
                     /locus_tag="PLY30_00625"
     CDS             complement(1887..2078)
                     /locus_tag="PLY30_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64633.1"
                     /translation="MKPIPVGPELMRMFHELHIEQPVPKDGPETSRSMMIKILDHDHP
                     RARHARHLPHQRLRVRHMM"
     gene            2234..2644
                     /locus_tag="PLY30_00630"
     CDS             2234..2644
                     /locus_tag="PLY30_00630"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FabA/FabZ family ACP-dehydratase"
                     /protein_id="HPW64634.1"
                     /translation="MSTVSIEKVLWIREGEGIAACGRIPSDSEFFRDHFPEFPVLPGV
                     LALEMLKQTAEKYLASLGNGAGGRYFLKHIRATRFTKYLRPGDEWESRLTLTQSSDEE
                     TAWDARLYYHGEVAVSARLTLARVKNPQSSATSL"
     gene            2664..3074
                     /locus_tag="PLY30_00635"
     CDS             2664..3074
                     /locus_tag="PLY30_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPW64635.1"
                     /translation="MNNEIFKKVQETLAGALGVDASDVTPDASLKRDLGAESIDFIDI
                     IFRLEKSFDLKIPANDLFPSHIFTNERFVKEGMVTPEGIHELRTKLPYLDLGAFDKDP
                     QVVKLADYFTVKMILRYLEERLGKSSGCSGTCKS"
     gene            3071..3445
                     /locus_tag="PLY30_00640"
     CDS             3071..3445
                     /locus_tag="PLY30_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPW64636.1"
                     /translation="MTVKTRRLHGVGVDLVSRSRMKMFLSRHRDMITRRFLNDSERRR
                     RTNIGLTELAELFTAKEAFFKACNEPWMGLGGFRGMRITERRGDGFTMEWKVAGTRRC
                     YRGEGVFFRDGDLVGAHTMIWS"
     gene            3448..3882
                     /locus_tag="PLY30_00645"
     CDS             3448..3882
                     /locus_tag="PLY30_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64637.1"
                     /translation="MRWLLLDKVLSIHRGKRAVTESRVPCAPYSSEVLFVEMMAQTGG
                     LLVGAESDYRNDVIFAKIESASFAGVPVPGAPITVEAFADGLAPEGGWIEGVITMAGV
                     VIAKAKLLLMSVGRLVPGHSQPITFHDNFMEHFKVREKVSVS"
     gene            3879..>5143
                     /locus_tag="PLY30_00650"
     CDS             3879..>5143
                     /locus_tag="PLY30_00650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPW64638.1"
                     /translation="MTRHRVVVTGFGVISACGNGWEPYWDSALQGRSAVREVERLKLN
                     GFSFKYAGTIRDFDPKQYVENRKSLKVMSREIQLAVGAAKLALQDAGLFGTAYDQSRF
                     GVTLGTGIINNDLDEIGVGFKNAIDGSGRFSMAKFGQEGVRSLFPLWFLKYLPNMPAC
                     HVSIAYHLRGPSNTITTSSAAGAQAIGEAYRVIERGDADIMLAGGTDSKLNAMGISRF
                     HLLGLLSKRAGLPEHVYTPFDERHDGIVLGEGAGILVLESLENAKRRGAPIYAEITGY
                     GSSSDFNFDPRDEDDFTGKRVAMRRALEDAGIEHHDVDVIVANGSGVPQGDIQEALAI
                     HSFCMGSFDKQSVTALKSVTGDLVYGSGGVEAVIAALIANKRVVPPVTNLKKPYPECP
                     LPLVRDKAKSADVTNVMFNNFGFGGQNASL"
BASE COUNT         1078 a         1365 c         1520 g         1180 t
ORIGIN      
        1 gccgatctcg ttgccgtcag cgccttcaat gtccaatacc gtcgcgcgat cgagatcgcg
       61 cgcgaattca gatcccgcgg ccggaaagtg gcgtttggcg gaccgtattg cagccttttt
      121 ccggaggcgt tcgagggcaa ggccgaccac cgcatcgtgg gggaggccga ggacatttgg
      181 ccgctttttc ttcgtgattt cgaagaaggc aaggcgcttg agctttatgt ggctccgaaa
      241 gcgagcgtgg atatccggaa gagccctgtc ccgcgttttg atcttattaa gggcgatcga
      301 tacaatatgt tttcccttca gacgactcga ggttgtccct ataactgtga attttgcgat
      361 atcatcatca tggacggccg gaaaccccgt acgaagaccg ttcagcaggt gcttgccgaa
      421 gtggaccatt gcgttcagca gggggcccat tacatcgtgt tcggtgacgc gaattttatc
      481 ggcaatatcg gctttgccag ggaactgctc acggccctcg cggattattc gaaaaagaac
      541 ggctacccga tcgagttctc atgcgaattg acgatcaatg tcgcccatca ccccgatctt
      601 ctggagcttc ttcaggaggc gaacttctac gccgttttcg tggggatcga atcgccgagg
      661 aagcaaagcc tcctcgaagc cggaaagtat cagaacacgc gcgaggacat catcgaagac
      721 atcaagcgta tccagtcgta tcacatttcg gttgttgccg gcatgatcgt cggctttgat
      781 tccgacgaca agggaatctt caaggagacc tacgattttc tccaggaact cggcattgcg
      841 tttacgacct gcgggacgct gatggccctg cctcacacgc cgctccttaa acgccttaag
      901 gccgaagggc ggcttctcga tctcgagtgg acgggcatga acggtcacgg cgcttcggac
      961 tgcaatttca tcccgaagct catgacgctc gacgaactgc gtaaaggcta taattggctt
     1021 agccgctgcc tctatcgtta cgatagttac gctgaccgcc ttgtcgccac gctgaaccgt
     1081 ttcaggaacc gtaataaaga acataaaaga gcgagtctcg acacgaaatt cctgagcgtt
     1141 ctcgtcaagg tcttcgccta ttacacgctg accttcgatc tcaaaaggat gtcgtttttt
     1201 tacggaacca tgtggcgtgt cgcggtgggc gggccctttt ctgtcgggaa atggctggaa
     1261 ttcttccgct ggatcgcgac gcatcgcgct tttcgccaat atgtgatgga aacccaggga
     1321 gatccggaag aaatggatcc caactcgcct ccgttcgatg tcgagtgtgc cccggtcacg
     1381 gacgacagtc cccgggaaga ggtgttggtt taaaagatgc gggattgtct cgggattgtc
     1441 gaggacccgg atccgcgggc gaaccggccg aatgctcctc tcttataaaa agatgcttgg
     1501 gagctaaaaa gtccctgtgt tataattccg aacccttgaa cagggcaggg accatttcta
     1561 ttgtggaatc gctctcaaat acacttcgga cataccagcg aaaactgttg gatacgttag
     1621 tcaggtgtca ttttcgtccg acgctcgaag cgagggcgga atcattcgtc aggctctttg
     1681 aaacgtttct cccgaaaggg agccggattc tggacctcgg cggcggctgg gggttctata
     1741 gcgaaccgct tgcccggcgc gggcacgatt cgctcgttct tgatgtcgta aagccgggat
     1801 atcaaaaggc accggttgtc atttacgacg ggagcaagat cccgttcggg gacaaaagct
     1861 ttgatgtaac cctccttgtg accatgttac atcatgtgcc ggacccggag gcgctgatgc
     1921 gggaggtgac gcgcgtgacg cgctcgcggg tggtcgtggt cgaggatctt tatcatcatg
     1981 gagcgggacg tttctggacc atccttcggg accggttgct caatatggag ttcgtggaac
     2041 atccgcatca gttccggacc cacggggatt ggcttcattt tttttgtagt aaggggttcc
     2101 ggcttgagaa gttcgcgcgg gtctatactt ggctcgcggg attccgtatt ctgaacggtg
     2161 tttatgtttg ggagtgtgaa ccatcgctcc gccattgtga acgatacgcg gggaatgaca
     2221 tcaagggcag gacatgtcca ctgtttcgat cgaaaaagtg ctctggatca gggagggaga
     2281 agggatcgcc gcttgcggcc gcatcccgag tgactcggag ttttttcgcg atcatttccc
     2341 cgagttcccg gtcctgccgg gcgtgctggc gcttgagatg ctcaaacaga cagccgagaa
     2401 gtacttggcg tcgcttggga acggcgcggg gggccgttat ttcctgaagc atatccgcgc
     2461 gacgcgtttc acgaaatatc tgcgtcccgg ggacgaatgg gaaagccggc tcacgctgac
     2521 gcagagttcg gacgaagaaa cggcttggga cgccaggctt tattatcacg gtgaggtcgc
     2581 ggtatccgcc agactgacgc tcgccagggt caagaatcct caatcatcgg cgacatcgtt
     2641 ataacgcaag gaaaaggaga atcatgaata acgagatttt caagaaagtg caggaaacat
     2701 tagccggcgc gcttggtgtt gacgcctctg acgtcacgcc cgacgcctcc ctgaagagag
     2761 accttggcgc cgaatcgatc gattttatcg acatcatctt ccgcctggaa aaaagtttcg
     2821 acctcaagat tcccgcgaac gaccttttcc cgagccatat cttcacgaac gaacgtttcg
     2881 tgaaagaggg aatggtcact ccggaaggga ttcatgaact ccgcacgaaa cttccgtacc
     2941 ttgacctcgg ggctttcgac aaggacccgc aggttgtgaa gctggccgat tatttcaccg
     3001 tcaagatgat cctccgttat ctggaagagc ggctgggaaa atcctcgggt tgttccggca
     3061 cctgcaaatc gtgacggtca aaacgcgccg tcttcatggg gtcggggtcg atctcgtatc
     3121 ccgttcgcgc atgaaaatgt ttctgtcccg ccatcgcgat atgatcacgc gcaggttcct
     3181 gaatgacagt gagcgccggc gccgcaccaa catcggtctc acggaacttg cggaactttt
     3241 caccgccaag gaagctttct tcaaggcctg taatgagccc tggatggggt taggaggttt
     3301 tcggggaatg cggatcacgg aacgtcgcgg cgacggattc accatggaat ggaaggtagc
     3361 tggcacccgg cgatgttatc gcggtgaggg ggtttttttc cgtgacggtg atctggtcgg
     3421 tgcccacacg atgatctgga gttgagtatg cgttggcttc ttcttgacaa agttctctcg
     3481 atccaccgtg gcaaacgagc cgtgaccgaa tcccgtgttc cctgcgcgcc ctactcaagc
     3541 gaagtccttt ttgtcgaaat gatggcccag accggaggtc ttctggtcgg agcggaaagc
     3601 gattaccgga acgacgtgat cttcgcgaag atcgagtctg cttcgttcgc cggggttccg
     3661 gtcccaggtg ctccgatcac ggttgaggcg ttcgcggacg ggctcgctcc cgagggtggg
     3721 tggatcgaag gtgtgataac aatggccggc gtggtcatcg cgaaggcaaa actgcttctt
     3781 atgtctgtcg ggcggctcgt cccggggcat tctcaaccca tcaccttcca tgataatttc
     3841 atggagcatt tcaaggtccg ggagaaggtg tcggtatcat gaccaggcac cgggtggtcg
     3901 tcacggggtt tggtgttatc agcgcctgcg ggaacggatg ggaaccctat tgggacagcg
     3961 ctctccaggg acgctcggcg gttcgtgaag tcgaacgtct caaactgaac ggattttctt
     4021 tcaaatatgc cggaacgatt cgggattttg accccaaaca atacgtggag aaccgaaaat
     4081 ccctcaaggt catgtcccgc gagatccagc tcgccgtggg ggccgcgaag cttgccctcc
     4141 aggatgccgg tcttttcggg accgcttatg accagtcccg cttcggtgtg acgctcggga
     4201 ccggtatcat caataacgat cttgacgaga tcggtgtggg tttcaagaac gcgatcgatg
     4261 gctcgggacg tttttcgatg gcgaagttcg gccaggaagg tgtccgctct ctttttccgc
     4321 tctggttcct gaaatacctt ccgaatatgc ctgcctgtca cgtttcgatc gcttaccatc
     4381 tccggggacc cagcaacacg atcacgacat cctcggcggc gggggcccag gcgatcgggg
     4441 aggcgtatcg ggtgatcgag cgcggcgacg cggatatcat gctggccggc gggaccgatt
     4501 caaaattgaa tgcgatgggg atctcccgtt tccatctgct cggtcttctc tcaaaacggg
     4561 ccggattgcc ggagcatgtc tacacgccgt tcgatgagcg tcacgacggg atcgtgctcg
     4621 gggaaggagc cggcattctt gttctggaat cgctggagaa cgcgaaacgg cgcggggctc
     4681 ccatctatgc cgagatcacg ggttatggtt cctcttcgga tttcaatttc gatccgcgtg
     4741 acgaggatga tttcaccggc aagcgggtcg cgatgcggcg agcgctcgaa gacgcgggga
     4801 tcgaacatca cgacgtcgat gtgatcgtcg caaacgggag cggcgttccc cagggggata
     4861 tccaggaagc ccttgccatc cactcgttct gtatggggtc gttcgacaaa cagagcgtga
     4921 ccgcgctcaa atccgtgacg ggagatctcg tctacggctc gggtggtgtc gaggctgtga
     4981 tcgccgcgtt gatcgccaac aagcgggtcg taccgcccgt gaccaatctt aaaaaacctt
     5041 atccggaatg tccgctgccg cttgtccggg acaaagccaa gtccgccgac gtcacgaacg
     5101 tcatgttcaa caacttcggt ttcggcggac agaacgcgtc act
//
LOCUS       DAOOBX010000021         5062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_18815_length_5062_cov_3.827242, whole genome shotgun sequence.
ACCESSION   DAOOBX010000021 DAOOBX010000000
VERSION     DAOOBX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18815_length_5062_cov_3.827242"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..954)
                     /gene="atpG"
                     /locus_tag="PLY30_00655"
     CDS             complement(94..954)
                     /gene="atpG"
                     /locus_tag="PLY30_00655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPW64639.1"
                     /translation="MSGQLRTIKNRIRSVESTKKITRAMEMVSAAKLKRFQKVLDQAA
                     PYTTALEGLLGRLIEAKTPFRHPLLTPRPEKKRALMVVTSDTDLCGSFNMDLVDEATR
                     FLGTLPETPVLVGFGKSGVNALGRMGLTCHTVFKDTKTADLEHAVKKTGLLLETLFRE
                     HKVDAVYVTYPRFMTKASFKAVTEKLLPLTLEAENAAVSSVEYIFEPGAEFLFTKLVP
                     MIFEAKVRELFMGSFVAEQTARMNAMHQATKNAGELIESLTLTRNKIRQAAITKELIE
                     IVSGSKAAKQ"
     gene            complement(1030..2535)
                     /gene="atpA"
                     /locus_tag="PLY30_00660"
     CDS             complement(1030..2535)
                     /gene="atpA"
                     /locus_tag="PLY30_00660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPW64640.1"
                     /translation="MLKPEEVTQVIEKEIRKYQTRIELESVGYVLQVGDGIARVYGLE
                     EAMAGELVIFHDGTPGMVLNLEHHHVGVVIFGADRQIKEGDQVKRTGKIAQVPVGDAL
                     KGRVVNPLGEPLDGKGPIASNKMRPVEAIPPSVVKRQPVKEPLQTGIKAIDAMIPIGR
                     GQRELVIGDRQTGKTAILLDTILHQKTTGVQCIYVAIGQKLSTVVTLAETLKKYGAME
                     YTTIVSASSEDPAPLQYLAPYAGTAIAEESMYSGKHALVVYDDLSKHAVAYRQMSLLL
                     QRPPGREAYPGDVFYLHSRLLERAAKLRDDLGGGSLTALPVIETQLGDISAYIPTNVI
                     SITDGQIYLESDLFYGGVRPAINVGLSVSRVGGNAQTKAMKQVAGMLRLSLAQYRELQ
                     AFAQLSTDLDKATQDQLARGERMTEILKQDDLSPMPLEHQVVIIFAGNEGLLDDIPVG
                     QISQFETRLLDFIDQNYLDIFHEISQKKVISDPLKEKMKQAVLKFKQTFKV"
     gene            complement(2547..3110)
                     /gene="atpH"
                     /locus_tag="PLY30_00665"
     CDS             complement(2547..3110)
                     /gene="atpH"
                     /locus_tag="PLY30_00665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPW64641.1"
                     /translation="MSETLASIRYARALFEISRKEDCLAETEAWLSLFEENAKRHPRI
                     NALMQNPIVSDAEKFTFLDKILPGDTLTILKNFLRVLIEKKRFALLGEIRQTFHALYD
                     RHRGSLEVVLISAVPLSEAVRNKLKETLAAKLGSSGGTGSPKEIHLVQKTEPSLIGGF
                     ILRFDGKEIDCSFKKRFREIEQQLLAQ"
     gene            complement(3107..3607)
                     /gene="atpF"
                     /locus_tag="PLY30_00670"
     CDS             complement(3107..3607)
                     /gene="atpF"
                     /locus_tag="PLY30_00670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPW64642.1"
                     /translation="MEYFQENIRLGEVFVQLLAFLIVFFTLRKFAWKPLTSVLKARRE
                     RIEHEWTGIEQMKAEVARLEKEYKEHLQKIDEEARVKMQEAIQEGRRVAREIQEKARG
                     EFQTSLEKTRANIEIEFEKARIELRREVAKLSLQVAEKVIREKMDAAHHEKKAMELIE
                     ELGKKP"
     gene            complement(3623..3853)
                     /locus_tag="PLY30_00675"
     CDS             complement(3623..3853)
                     /locus_tag="PLY30_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPW64643.1"
                     /translation="MNVQIVLGFTLPLGLAITAFGSAIALGKAVAAAMDATSRQPEAS
                     GKILVNMMAGCALIEALTLYALVFGFVLMGKI"
     gene            complement(3870..4718)
                     /gene="atpB"
                     /locus_tag="PLY30_00680"
     CDS             complement(3870..4718)
                     /gene="atpB"
                     /locus_tag="PLY30_00680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPW64644.1"
                     /translation="MDQTGHDIAQTAHATAQAVGEAHELPNFLTVLAKLFPENPVFDF
                     LHRWENIVFSLLVIGFIGWRAIAAARTGRFIPKGAQNAWEAVTEGLTSFVTGILGPRG
                     VAHVPFLGTLFIYILFDNWLGLVPFLKSPTSGWSTTIALAIVTIVYVHATGIREQGPL
                     NYAKHMMGSPQGIVGIALAPLMLVINITVDYLAVPLSLSLRLFANISSEDRLLYKFAE
                     LNVMYKMLPFFFQLFANVLVIIFSLVQAFVFLLLSTVYISLIMPREDHDQDGKAHEKK
                     AIAIHH"
     gene            4761..>5062
                     /locus_tag="PLY30_00685"
     CDS             4761..>5062
                     /locus_tag="PLY30_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64645.1"
                     /translation="MVWIEDPRRPKSTDIIINFGLKFHCLINQWASRNPAKGPEIRIA
                     GIVTATPSILKSLGRSFVPAMTFHYPIVSESFNRNADTRRQNKGEVMITAFIKLVF"
BASE COUNT         1001 a         1361 c         1553 g         1147 t
ORIGIN      
        1 tgcctgatga cataagctct ccttggtacg aagcatggga tgcgaagcac cgagcccttt
       61 gcttgcgcaa gactccgtac tccgtacgcc gtactattgt ttagctgctt tcgatcccga
      121 aacgatctcg atcagttctt tggtgatggc ggcctgacgg atcttgttcc gcgtgagcgt
      181 gagcgattcg atcagctcgc cggcgttctt cgtggcctga tgcatcgcgt tcatgcgagc
      241 ggtctgttcg gccacaaacg atcccatgaa aagttcccgg acctttgcct cgaagatcat
      301 ggggaccagt tttgtgaaaa ggaactccgc gcccggttcg aagatgtatt cgaccgagga
      361 gacggccgcg ttttcggctt ccaacgtcaa ggggaggagc ttttccgtga cggccttgaa
      421 cgaggccttt gtcataaagc gcgggtaagt gacatagacc gcatcgacct tgtgttcgcg
      481 gaaaagggtt tcaagcagca acccggtttt cttgaccgcg tgttccaggt ccgctgtttt
      541 cgtgtccttg aaaacggtgt ggcacgtcag gcccatgcgg ccgagggcgt ttacgccgct
      601 cttcccgaac ccgacgagga ccggcgtttc cggtaaagtc ccgaggaaac gcgtcgcctc
      661 atcgacgagg tccatgttga acgatccgca aaggtccgtg tccgatgtta cgaccatgag
      721 cgcacgtttc ttttccggcc gcggggtgag caggggatga cgaaagggcg ttttcgcttc
      781 gatgagcctg ccgaggaggc cctcgagcgc cgtggtgtaa ggcgcggcct gatcgaggac
      841 cttctggaag cgcttcagct tcgcggcgga gaccatctcc atggcacgcg tgatcttttt
      901 cgtgctctcg acgctgcgga ttctgttttt aatcgttcgt agttgaccgc tcataatcct
      961 tgtgcggagc gcgggggccg gagcacggag tttcgttttt ttacagaact ccgtacgccg
     1021 tacaccgcac tagactttaa aggtttgttt gaacttcagg accgcctgct tcattttctc
     1081 tttcagcggg tccgaaatga cttttttctg tgagatttca tgaaagatat caaggtagtt
     1141 ctggtcgatg aaatcaagga gccgggtttc gaactggctg atctgcccga cgggaatatc
     1201 gtcgaggagt ccttcgttgc cggcgaaaat gatgaccacc tgatgttcca gcggcatggg
     1261 agagagatcg tcctgcttca gaatctcggt catgcgctcc ccccgcgcga gctggtcttg
     1321 cgtagccttg tcgaggtcgg tggaaagctg cgcgaaggcc tggagctcgc ggtactgggc
     1381 aagcgaaaga cgcagcatgc cggccacctg tttcatggcc ttggtctggg cgttcccgcc
     1441 gacgcgcgac acggaaagac cgacattgat cgcggggcgg actccgccgt aaaagaggtc
     1501 gctttccaga taaatctggc cgtccgtgat cgaaatgacg tttgtcggga tgtaggccga
     1561 gatgtcaccg agttgggtct cgatcacggg cagcgcggtg agcgagcctc cgccgaggtc
     1621 gtcgcggagc ttcgcggcgc gctcgagcag gcgggaatgg agataaaaga catcgccggg
     1681 atatgcctcg cggcccggag gacgttgcag aaggagcgac atttgccggt aggcgaccgc
     1741 gtgttttgaa aggtcgtcat agaccacgag ggcgtgtttg ccggagtaca tggattcctc
     1801 ggcgatcgcg gtgccggcgt acggcgcgag gtactggagc ggcgcgggat cctccgagga
     1861 ggccgacacg atcgtcgtgt attccatcgc gccgtatttt ttgagcgttt cggcaagcgt
     1921 gacgacggtc gaaagcttct ggccgatcgc gacgtagata cactgcactc cggtcgtctt
     1981 ctggtgaagg atcgtgtcga ggaggatagc ggtctttccc gtctggcggt caccgatcac
     2041 gagctcgcgc tggccgcggc cgatcgggat catcgcgtcg atcgccttga tgccggtctg
     2101 gagcggttcc ttgacgggct ggcgtttgac gaccgagggc gggatcgcct cgaccgggcg
     2161 catcttgttt gacgcgatcg ggcccttgcc gtcgagcggt tcgccgagcg ggttgacgac
     2221 gcggcccttc agggcgtcgc cgaccgggac ctgggcgatc ttgcccgtgc gcttgacctg
     2281 atcaccctct ttgatctgac ggtccgcgcc gaagatcacg acaccgacat gatggtgctc
     2341 gagattcagc accatgccgg gggtcccgtc atgaaaaatc acgagttcgc cggccatggc
     2401 ctcttcgagt ccgtagacgc gtgcgattcc gtcgccgacc tgaaggacgt aaccgacaga
     2461 ctcgagctcg atcctggtct ggtattttcg gatctctttt tcgatgacct gcgtgacttc
     2521 ttcgggttta agcatcggga ccttccttat tgagcaagta gctgttgttc gatctcacga
     2581 aaacgtttct tgaacgagca gtcgatctct ttgccgtcaa agcggaggat gaacccgccg
     2641 atcagggacg gctcggtctt ctgcaccaga tgaatctcct tcgggcttcc ggttccgccg
     2701 gaggaaccga gcttcgcggc gagagtttct ttgagcttgt tccggaccgc ctccgaaaga
     2761 gggaccgcgg agatcaatac cacttcgagc gagcctcggt ggcggtcgta cagggcatga
     2821 aaggtctgcc ggatctcacc gaggagcgcg aaccgttttt tctcgatcag gacgcgcagg
     2881 aaatttttga ggatggtgag cgtgtcaccc gggaggatct tatcgaggaa cgtgaatttt
     2941 tcagcatcgc tgacgatcgg gttctgcatc agggcattga tccggggatg ccgtttcgca
     3001 ttctcctcaa aaagggaaag ccacgcctcg gtttcggcca ggcagtcctc cttccgggaa
     3061 atctcgaaga gggcgcgggc gtagcggatg ctcgcaaggg tctcgctcat ggtttcttcc
     3121 cgagttcttc gataagctcc atcgcttttt tctcgtgatg ggccgcgtcc attttttcgc
     3181 ggatcacctt ttcggcgacc tggagcgaaa gcttcgcgac ctcgcggcgg agttcgatcc
     3241 gggccttctc gaactcgatc tcgatgttgg ccctggtttt ttcgagagag gtctggaatt
     3301 ccccccgcgc cttctcctgg atctcgcgcg caacgcggcg gccttcctgg atggcctcct
     3361 gcatcttcac gcgtgcctcc tcgtcgattt tctgaagatg ttccttgtat tccttctcga
     3421 gtcttgcgac ctcggccttc atttgctcga tacccgtcca ctcatgttcg atccgctcgc
     3481 ggcgcgcctt gaggacgctg gtgagcggtt tccaggcgaa cttcctgagc gtgaaaaaca
     3541 cgatcagaaa ggcgagcagc tggacgaaga cctcacctaa acggatgttc tcctgaaaat
     3601 attccatgga gcctttcggg ggttaaatct tgcccatgag cacgaagccg aatacgagcg
     3661 cgtaaagcgt caaggcttcg atcagggcgc atccggccat catattgacg agaatcttgc
     3721 ccgacgcttc aggctggcgg ctggttgcat ccatggcggc ggccacggcc ttcccgagcg
     3781 cgatcgcgga accgaatgca gtaatggcga gtccgagagg taatgtgaaa cctaaaacga
     3841 tctgaacgtt catgaacagt ctcctttggt tagtgatgaa tagcgattgc ctttttttcg
     3901 tgggccttcc cgtcctggtc atgatcctca cggggcatga tcagggaaat gtacacggtc
     3961 gagagcagca ggaacacgaa cgcctggacc agactgaaaa tgatcacgag cacgttcgcg
     4021 aaaagctgga agaagaaggg gagcatcttg tacatcacgt tgagctcggc gaacttgtaa
     4081 agcagacggt cctcgctcga gatattggcg aaaagacgga gcgagagcga aagcggcacg
     4141 gcgaggtaat cgacggtgat gttgatgacc agcatgagcg gtgcgagcgc gatccccacg
     4201 atgccctggg ggcttcccat catgtgtttg gcataattga gagggccttg ctcccggatt
     4261 cccgttgcgt ggacgtagac gatcgtcacg atggcgagcg cgatcgtggt gctccatccg
     4321 cttgtagggg atttcagaaa agggacgagg ccgagccagt tgtcaaacag tatatatatg
     4381 aagagggtgc caaggaacgg gacgtgcgcg acccccctgg ggccgaggat gccggtcacg
     4441 aaggatgtga ggccctcggt aacggcttcc cacgcattct gtgcgccctt cgggatgaac
     4501 cgcccggtcc tggccgcggc gatcgctcgc cagccgatga atccgattac gagcagggag
     4561 aagacgatat tctcccagcg atggagaaaa tcgaagaccg gattttcggg gaagagtttg
     4621 gccaggaccg tcaggaagtt gggaagctcg tgagcctccc cgacggcttg tgcggtcgca
     4681 tgagcggtct gggcaatatc gtgtccggtc tggtccatgg ctatgggctg atgtccttgt
     4741 agaggtgttt tatgagccgt atggtctgga tcgaggatcc gagaagaccc aaaagcaccg
     4801 atatcataat caatttcgga cttaaattcc actgcttgat taaccaatgg gcgagcagga
     4861 acccggccaa aggaccggaa ataaggattg ccgggattgt gaccgcgact ccgagcatcc
     4921 tgaagagcct ggggaggtcg tttgtgcccg ccatgacgtt tcattatccg attgtttctg
     4981 aaagtttcaa cagaaatgcc gatactagga gacagaacaa gggggaagtc atgatcaccg
     5041 cattcatcaa attggtgttc ca
//
LOCUS       DAOOBX010000022         4996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_19125_length_4996_cov_8.352560, whole genome shotgun sequence.
ACCESSION   DAOOBX010000022 DAOOBX010000000
VERSION     DAOOBX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19125_length_4996_cov_8.352560"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1085)
                     /locus_tag="PLY30_00690"
     CDS             complement(102..1085)
                     /locus_tag="PLY30_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HPW64646.1"
                     /translation="MPQRRLKPILDKDRYSDEIIKELDFHLYDLIYRPLLELLEDETG
                     LEVKWNTKKGLIAALHGGKIQYRDGYFTGQFSSSISKTIRDFGGTWDKHRKSFKIDLA
                     ALPIDIKAAVSASKLKTERVQKQLELELDRIEAANDQLETYQIPFGPKVEKILQELDS
                     QFQKVTPKDIMIEPQLDGGVAERLQEQYNKNLNYYIKGWRKDAIYRLREKMDENVFSG
                     FRAKRMVDTIQSEYGVTKNKAKFLARQETSLLLSKYRQERYEKIGIGRYVWVTSHDAR
                     VRHRHKDLDGRIFSWDNPPIVDQATGRRANPGEDFNCRCVAQPVFDDDELN"
     gene            complement(1206..2624)
                     /locus_tag="PLY30_00695"
     CDS             complement(1206..2624)
                     /locus_tag="PLY30_00695"
                     /inference="COORDINATES: protein motif:HMM:NF018125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HPW64647.1"
                     /translation="MKKIRRAKNAKVKAVKGANRVRALRNAYSPSPANDMLSGVFGGF
                     MGGNQAPLSNPYGMSYDANFSPLSLNRILLSYTYFTHGPIQTAIDQPVEDAFRGGLEL
                     TCDELDSEDLKRLNDYMNRHIIPVLKDGMRWAKLFGGAGLILNTDQDPETEFKPDSLG
                     QNDLFALIAADRWELTLNYAAISKSETPYNYYGQKMHKSRVLKINGKEAPSFIRARLQ
                     GWGMSEIERMIRPAQAFLKNEDLIYELVDEAKIDVVKIQGFNDQQLSGLAQTMTSEFL
                     RVAAMTKNFHNMLAIDKEDDYDQKQISFSGLPEILNQNRISMAAAARMPVTKLFGLSA
                     AGFNSGEDDIENYNALIESEVRGQATEILLELLPIACRVLFGFAPDVEFKFKPLRILS
                     TEQEETVKTNKFNRLSALYSQGILNPVEYCEALKKEGIFVMETEVSKGIKDPEVPMSS
                     MSTDQPQDSAREEAPKGNSKRR"
     assembly_gap    2673..2772
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2773..3929)
                     /locus_tag="PLY30_00700"
     CDS             complement(<2773..3929)
                     /locus_tag="PLY30_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HPW64648.1"
                     /translation="MIPEKIECEYPEIFHPFFAEPKRYNVAEGGRGRGASWTIARKLL
                     IEASRSRQRVLCTREYQNSIRDSVHRLLVDQIYALHLEDFFTIFKDSIICPIFRSEFI
                     FKGLHTAIQEIKSLEGITRCWVEEAEAVSEYSWTVLIPTIRAQGSQFYVSYNSESENS
                     ATYIRFHKNPPPDSNIVFSTYADNPWFPETLRREMEYDKRVDYEKYEHVWLGLPKTYG
                     EALIMRGKIKVEAFETPPDAQLLFGADFGFSVDPTVLLRCFIKGTKLFIDYEAYGHGI
                     ELMELENFFLTVPESRRTLGGPKWEIRADSARPETISLLKRQGFNIVGAEKGKGSVED
                     GIQFLRSFEEVIIHPRCKGTISDFKNWRWKQDRITGRVIPIPAEGSDHSPDA"
     gene            complement(3919..4374)
                     /locus_tag="PLY30_00705"
     CDS             complement(3919..4374)
                     /locus_tag="PLY30_00705"
                     /inference="COORDINATES: protein motif:HMM:NF039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HPW64649.1"
                     /translation="MPGPNSQKNSRETVMRENPAEHLKPYQFKPGQSGNPTGRPPEAP
                     AELKNYTKKEIAEVMCRMMKMTVDELVRFSKRPEATALEMIIASVIRECMKKGDFHTL
                     DKMLERVIGKVPQKTELTGNEGEPFFIPPAVFAPVRPSKAAEPEKPNDP"
     gene            complement(4340..>4996)
                     /locus_tag="PLY30_00710"
     CDS             complement(4340..>4996)
                     /locus_tag="PLY30_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPW64650.1"
                     /translation="MSEASFREFLKKTMENYRDFSNPNAALYCCYADRTHREFEDALN
                     HAGWEVKNQIIWVKAVASMGWGDYRLKHEPMFYAHQKGSKLNFYGDWCQYTEWTEKKT
                     DAELLDLFKKLIEKDEKGGSTIWRFSREHNYKHPTQKPVELVKKAISNSSQRGEIVLD
                     LFGGSGSTLLACDLLKRQCRTMELDPKYVDVIIERWCTFTGNDREAVYARAQQPEKQP
                     "
BASE COUNT          839 a         1381 c         1415 g         1261 t
ORIGIN      
        1 ccaaaactgg caggtgtatt ccttcgggcc gtcgtttttc ttcgcgttcg ttttttggga
       61 tttcagaata atgcggtatg tcattttagg ttccctcgtt tttagtttaa ctcatcatcg
      121 tcaaagactg gctgggcgac gcaccggcag ttgaagtcct cgccggggtt tgcgcgtcgg
      181 ccagtggctt ggtccacgat cggcggattg tcccaggaaa aaatgcggcc gtccaggtcc
      241 ttgtgtcggt gccggacgcg ggcgtcgtgg ctcgtcaccc acacgtatcg gccaatcccg
      301 atcttctcgt agcgttcctg gcggtatttc gatagcaaca gcgacgtttc ctgccttgcg
      361 aggaacttcg ccttgttctt ggtcactccg tactcgctct ggatcgtgtc gaccattcgc
      421 tttgcgcgga acccggagaa cacgttctcg tccatcttct cgcggagccg gtagatcgcg
      481 tccttgcgcc agcctttgat gtaataattc aggttcttgt tgtattgctc ttgcaagcgt
      541 tccgcgacgc cgccgtcaag ctggggctcg atcatgatgt ctttgggcgt caccttctgg
      601 aactggctgt ccagttcctg caggatcttc tcgactttgg gcccgaacgg gatctgatag
      661 gtctcgagct ggtcgttggc ggcctcgatc ctgtcgagct cgagctcaag ctgtttttga
      721 acgcgctcgg tcttgagctt gctcgctgaa acggcggctt tgatgtcaat ggggagagcg
      781 gctaggtcaa ttttaaagga ttttctgtgt ttgtcccagg tccccccaaa atcacgaatg
      841 gtctttgaga tgctcgagct gaattgtccg gtgaagtatc cgtcacggta ttgaatcttc
      901 ccgccgtgga gcgcggcgat caggcccttc ttggtgttcc acttcacttc aagcccggtc
      961 tcgtcctcca aaagctcaag cagtgggcgg tagatcaaat cgtaaaggtg gaagtcgagc
     1021 tccttgatga tttcgtcgga gtagcggtcc ttgtcgagga ttggcttcag gcggcgttgt
     1081 ggcatgggtt agcctacccg catgattcgg agttcgcctc gggcctcgta taaaaaatag
     1141 ctggcgcggt tgtggtaccc gaggaagtaa aacgtcataa atcccgctga gtgtgggggc
     1201 atcggttacc tccgcttgct gttgcctttg ggcgcttcct cgcgtgcgga gtcctgaggc
     1261 tggtcggtgc tcatggagct catgggaacc tcggggtctt tgatcccctt cgagacttcc
     1321 gtttccatga caaaaatgcc ctctttcttc agggcttcgc agtattcgac agggttcaga
     1381 atgccctggc tgtaaagcgc agacaggcgg ttgaacttgt tcgtcttgac cgtttcctct
     1441 tgctcggtcg acaggatccg gaggggcttg aatttaaact ccacgtctgg cgcgaagccg
     1501 aaaagcaccc ggcaagcgat cggaagaagc tcgagcagga tctcggtcgc ctggccccgg
     1561 acctcgcttt cgatcagcgc gttgtagttc tcgatgtcgt cctcgccgga gttgaagccg
     1621 gcggcggaaa gcccgaagag tttcgtcacg ggcattcttg cggccgcggc catgcttatc
     1681 ctgttctggt ttaggatctc tggcaagccg gagaagctga tctgcttctg gtcgtagtcg
     1741 tcctctttgt cgatcgcgag catgttgtgg aagttcttcg tcatggcggc cacgcgcagg
     1801 aattcgctgg tcatggtctg cgcgagcccg gagagttgct ggtcgttgaa cccctggatc
     1861 ttcaccacgt cgatcttggc ttcgtcgacg agctcgtaga tcaggtcctc gttcttgaga
     1921 aacgcctgtg ccgggcggat catgcgctcg atctcggaca tgccccagcc ctggagccgg
     1981 gcccggatga agctaggagc ttctttcccg ttgatcttga gcacccggct cttgtgcatc
     2041 ttctggccgt agtagttgta cggggtctcg ctcttggaaa tggcggcata gttcagcgtc
     2101 agctcccagc ggtccgcggc gatcaacgca aagagatcat tctgcccgag gctgtccggc
     2161 ttgaattcgg tctcggggtc ctggtccgtg ttcaggatca gtccggcccc gccgaataac
     2221 ttcgcccagc gcatgccatc cttcagcacg ggaatgatgt gccggttcat gtaatcattc
     2281 aggcgcttca ggtcctcgga gtccagctcg tcacatgtca gttctagtcc acctctgaac
     2341 gcgtcctcga ccggctgatc gatcgcggtc tggatcgggc cgtgagtaaa ataggtatag
     2401 ctcaggagga tccggttcag ggagagcggc gagaagttcg cgtcatacga catgccgtaa
     2461 gggtttgaaa gcggcgcctg gttcccgccc atgaatccgc cgaacacgcc ggagagcatg
     2521 tcgttggccg ggcttgggct gtaggcgttt cggagcgccc tcactcggtt cgcgcccttt
     2581 actgccttca ccttcgcgtt ctttgctcgt cggatctttt tcatgatttc ctcgctagtg
     2641 cgtcgtagat cgtggttttc tttttcatgt atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nngcgtcagg gctatggtcc gagccctctg ctgggatcgg gataactcgc
     2821 cccgtgatcc ggtcctgctt ccagcgccag ttcttgaaat cactaatggt tcccttgcac
     2881 ctggggtgga tgataacttc ctcaaaagag cgcaaaaact gaatgccatc ctctacggat
     2941 cctttccctt tctccgcgcc gacaatgtta aagccctggc gctttaggag cgatatagtc
     3001 tcgggccgtg cgctgtccgc ccggatctcc catttcggtc cgccgagcgt cctccggctt
     3061 tccggcacgg tcaggaagaa gttctcgagc tccatcagct cgatgccgtg cccgtatgcc
     3121 tcgtaatcga taaagagctt ggtccccttg ataaaacaac ggaggagcac ggtcgggtcg
     3181 accgagaagc caaagtccgc gccgaagagc agttgcgcgt ccggcggggt ctcgaacgct
     3241 tccaccttta ttttacctcg cataataagc gcttctccgt aggtcttggg gagcccgagc
     3301 cagacgtgct cgtatttctc gtaatcgacg cgtttgtcgt actccatctc ccggcgcagg
     3361 gtttcaggga accacgggtt gtcggcgtaa gtcgagaaca cgatgttgct gtccggcggc
     3421 gggtttttat ggaaccggat gtaagtcgcg ctgttctcgc tctccgagtt gtagctcacg
     3481 taaaactgcg agccctgggc gcggatcgtt gggatcagca cagtccacga gtattcgctc
     3541 acggcctcgg cttcctccac ccagcaccgg gtgattcctt cgagggattt gatctcctgg
     3601 atcgcggtgt gcaggccctt aaaaataaac tcggagcgga agatcgggca aatgatcgag
     3661 tccttgaaga tcgtgaagaa gtcctcaagg tgcagggcgt agatctggtc cacgagcagg
     3721 cggtgcacgg aatcgcggat tgagttctgg tattccctgg tgcagaggac gcgctggcgg
     3781 gatctcgacg cttcgatcag gagcttacgg gcaatggtcc agctcgcgcc tctcccgcgg
     3841 ccgccttccg cgacgttgta gcgcttgggc tcagcgaaga acgggtggaa gatctccggg
     3901 tattcgcatt cgatcttttc agggatcatt cggtttctcc ggctccgccg ctttcgacgg
     3961 gcgcaccgga gcgaacacgg caggcgggat gaagaacggc tccccttcgt tcccggtgag
     4021 ctctgtcttt tgcgggacct tcccgatcac gcgctcgagc atcttgtcta aggtgtggaa
     4081 gtcccccttc ttcatgcatt cccggatcac gctggcaatg atcatttcga gggctgtggc
     4141 ttccgggcgc ttgctgaagc ggacgagctc gtcgaccgtc attttcatca tccggcacat
     4201 aacctccgcg atctcttttt tcgtgtagtt cttgagctcg gcaggcgctt ccggcggccg
     4261 gccggttggg tttccgctct gccctggctt gaattggtac ggtttcaggt gctctgctgg
     4321 gttctctctc ataactgttt cacggctgtt tttctggctg ttgggccctg gcatagaccg
     4381 cttcccggtc gttcccggta aaggtgcacc agcgttcgat gatcacgtcg acgtattttg
     4441 ggtccagctc cattgtccgg cattgccgtt tcaagaggtc acaggctagg agcgtggagc
     4501 cggagcctcc aaagagatca agaacgatct ccccgcgctg ggatgagttc gagattgctt
     4561 ttttgacgag ctcgacgggc ttctgcgtcg ggtgcttata attatgctca cggctgaagc
     4621 gccagatcgt ggagccgcct ttctcgtctt tctcgatcag cttcttgaaa aggtccagga
     4681 gctccgcgtc cgtctttttc tccgtccatt ccgtgtattg acaccagtcc ccgtagaagt
     4741 tcagcttcga gcctttctga tgcgcgtaaa acatcggctc atgcttcaag cggtaatctc
     4801 cccaccccat acttgccacg gccttcaccc atatgatctg gtttttcact tcccacccgg
     4861 cgtggttcag cgcgtcctca aactcgcggt gcgtgcggtc ggcgtagcaa cagtaaagcg
     4921 cggcgttcgg gtttgaaaaa tcccggtaat tctccatcgt ttttttgaga aactcgcgga
     4981 acgaggcctc gctcat
//
LOCUS       DAOOBX010000023         4995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_19135_length_4995_cov_3.410324, whole genome shotgun sequence.
ACCESSION   DAOOBX010000023 DAOOBX010000000
VERSION     DAOOBX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19135_length_4995_cov_3.410324"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLY30_00715"
     CDS             <1..846
                     /locus_tag="PLY30_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPW64651.1"
                     /translation="LAKKLRRPATVHKGDMGRVFILAGSRGMIGAADLAGMAALRSGA
                     GLVTLGIPDAIYLIIARRNSELMTRPLPSTSKGTLALSAFPEIRKFCRTQDVMAIGPG
                     LSQHVTTQKLVRRVSADTALPLVIDADGLNALQGHLDLFKKRTAPSVLTPHPGEFVRL
                     FDGKLDTSDAVRKKRAAEAAKEHGVVIVLKGHHTVVADPAGEIYVNKTGNPGMATAGS
                     GDVLTGVIAALAGQGFSLYEAACLGVHVHGLAGDLAAKAVGKTSLIAGDILMSLPDAF
                     KEVVP"
     gene            928..1863
                     /locus_tag="PLY30_00720"
     CDS             928..1863
                     /locus_tag="PLY30_00720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW64652.1"
                     /translation="MNTDLYGHCQLCPRDCGVDRTRGRMGVCGETAELRISHIGPHTG
                     EEPSFSGTRGSGTIFFCGCSSHCFFCQNHQISTNHRGTVYSAEQFFREVKTLIESKVH
                     NLNFVTPDHFWPHIKELCFRLREEEILIPKLFNSSGYQKEALIPEYADFMDIFLPDMK
                     FADGALAKLCMNDENYPGIAMAAIRRMVRLKGFLDLWDPEGNQTARRGVLVRHLVLPG
                     HVENSLKVLDLLYGEFGPGIPISVMSQFCPVPACSRMRLMTRGLLPEEYGRVLEHIDK
                     LGFEKVYTQVLSEKTEYLPDFNDPDDPFPGHRKKA"
     gene            1973..2668
                     /locus_tag="PLY30_00725"
     CDS             1973..2668
                     /locus_tag="PLY30_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPW64653.1"
                     /translation="MPLMMSPMTKSFQVSPSGLSLFKDCPRCFWLEKVRYIKRPRGIF
                     PSLPGGMDRVIKLYFDGFRSKGTLPPELASREEFRGLCLYKDLAQLERWRDWKTGLEY
                     FENGGRLFGALDDLLVAGDVCVPFDYKTKGLPTSEEDATKYYQTQLDCYALMLEANGM
                     KTAGYGFLLYYSPREVEENGRVIFEIQPIRIGTDIERARSYFRRSAAVCEGPAPAASA
                     NCEYCGWLNKFRK"
     gene            2746..3030
                     /locus_tag="PLY30_00730"
     CDS             2746..3030
                     /locus_tag="PLY30_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010471158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW64654.1"
                     /translation="MTEKTEWKVLVEKPAPEKLKSLGVMNWPIWMKEVSVFDWSYDKT
                     EVCYFLEGDVTVTIPGGEVTIGKGDLVTFPEGLHCTWRIKKAVKKHYKFG"
     gene            3084..3506
                     /locus_tag="PLY30_00735"
     CDS             3084..3506
                     /locus_tag="PLY30_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64655.1"
                     /translation="MVLPVPKTNAMLICDYVITERGTNKKSLIGVFENIGAMKFPCIH
                     AAMSIYVKLTDAQGSYRVRLDLVDLGEDKTIGQNEIPNDVQVPSPLVSHELVFNLRDI
                     HFTHPGEYEFRIFANGSIFGQKKFTVEQARRNNAEGVP"
     gene            3506..4060
                     /locus_tag="PLY30_00740"
     CDS             3506..4060
                     /locus_tag="PLY30_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPW64656.1"
                     /translation="MPKTAVVILAEGFEEIEAIGPVDVLRRAGVRAILAGVDGLTVKS
                     SRGVPVLAEILLKDVKGLPDAAIVPGGLPGATHLAKSAVVGEFLKKMNSDKRLIAAIC
                     AAPSTVLSPLGILDGKKATCYPGCETDFAPGVRHSADRVVVDGHIITSQGPGTALEFA
                     IAVTEALAGKEMAGTVRAKMLIGA"
     gene            4066..4572
                     /gene="cyaB"
                     /locus_tag="PLY30_00745"
     CDS             4066..4572
                     /gene="cyaB"
                     /locus_tag="PLY30_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HPW64657.1"
                     /translation="MTVYEIEQKYRLKDLAAVRRGLRALGAKKIASGRETNEFFDRNG
                     ELRKRKIAMRLRRYDGQSTLTLKGPKLRSRFTKRVELEMPVEHSPLRSILLLSGFRPV
                     MKYTKNRELYGLGKARVTIDRLRGFGCFLEIEGTVSVIARIASALGLGDGDREPRSYL
                     QMMFGWKH"
     gene            4586..>4995
                     /locus_tag="PLY30_00750"
     CDS             4586..>4995
                     /locus_tag="PLY30_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPW64658.1"
                     /translation="MIIATELRVGNVFKSDGKLFKVITQEVKGTGKFGKSVHCKVRSI
                     EDGNVAEKSFRAEDKIEDVPVRIVKMQYLYRESDQFVFMNMENYEQFNITARAVGRHE
                     VFLKENEAIEVLFGEGRVLSIMFPKIVELKVTSTP"
BASE COUNT         1110 a         1302 c         1513 g         1070 t
ORIGIN      
        1 ctcgcaaaaa agctcagacg tccggccacg gttcataaag gcgatatggg gcgggtcttc
       61 attcttgccg gttcccgcgg catgatcgga gccgcggatc tcgcgggcat ggcggctcta
      121 agatcgggcg cggggcttgt gacgctcgga atccccgatg cgatctatct gatcatcgca
      181 cgccgcaatt ccgaacttat gacgcgtccg ctcccgtcca cctccaaagg gacgctcgcg
      241 ctttcggctt ttccggaaat ccggaaattc tgccgcacgc aggacgtgat ggcgatcggt
      301 cccggacttt cacagcatgt gacgacgcag aagctggtcc gtcgggtgag cgcggacacg
      361 gcgttgccgc ttgtgatcga cgctgatggt ttgaacgccc ttcaagggca tctggatctt
      421 tttaaaaaaa ggacggcgcc cagtgtcctg acgcctcatc ccggagaatt cgttcgtttg
      481 ttcgacggga aactcgatac ctcggacgcc gtgagaaaaa aacgcgccgc cgaagccgcg
      541 aaagagcacg gcgttgtgat cgttctcaaa ggtcatcaca cggtcgtggc ggatcctgcc
      601 ggagagattt atgtgaacaa gacagggaat cccggcatgg caacggcagg ttccggcgat
      661 gtgctcaccg gagtgatcgc cgctttggcg ggtcagggtt tttcccttta tgaagcagcc
      721 tgcctcgggg ttcatgtcca cggccttgcg ggcgatctcg cggcgaaagc ggtcggcaag
      781 acaagcctga tcgcaggcga tatcctgatg tccctgccgg atgcctttaa agaagtcgtt
      841 ccctgatccc gggtagcgga aataactgaa cctgatgatc tcatccagcg ccgtatccaa
      901 tgatcttccg accgggtctt tgaaacgatg aatacggatc tttacggcca ttgtcagctt
      961 tgcccgcgcg attgcggggt tgaccggacc cgcggccgga tgggtgtttg cggcgagacc
     1021 gcggaactcc ggatttccca tatcggacct cacaccggcg aggaaccgtc cttttccggg
     1081 acgcgcggtt cggggaccat ttttttttgc ggctgctcaa gtcactgttt tttttgccag
     1141 aaccatcaga tctccacgaa tcaccggggg acggtctatt cagcggagca attcttccgc
     1201 gaggtcaaga cgctcatcga aagcaaggtc cacaatctca acttcgtgac cccggaccat
     1261 ttctggcccc atatcaagga actttgtttc cggctcaggg aagaggagat cctgatcccc
     1321 aagctcttta acagctcggg gtatcaaaag gaggcgttga tcccggagta tgcggatttc
     1381 atggatatct tccttccgga tatgaaattc gcggacggcg cgctcgcgaa actctgcatg
     1441 aatgatgaaa attatcccgg gatcgcgatg gcggcgatcc ggcggatggt acggttaaaa
     1501 ggattcctcg acctctggga ccccgaggga aaccagaccg cgcgccgcgg ggtcctggtt
     1561 cgccatcttg tccttcccgg ccacgttgag aacagcctga aggtcctgga ccttctttac
     1621 ggcgaattcg ggcccgggat cccgatttcc gttatgagcc agttttgtcc ggtacccgca
     1681 tgctcacgga tgcgtctgat gacgcgcgga cttcttccgg aagagtacgg ccgggtgctc
     1741 gaacatatcg acaagctcgg tttcgaaaag gtctacacgc aggtcctgag cgaaaagaca
     1801 gagtatctgc ccgactttaa cgatccggac gatccctttc cgggacatcg gaaaaaggca
     1861 taggggactc gctacccgga agcagggacc cgaaaacagt tttcgggcga tcagccccga
     1921 aggactggtt tttgctgttg agcgttgtgt attgagcata aaaagatata taatgcccct
     1981 gatgatgagc ccgatgacaa agagtttcca ggtgtccccg tctggtctta gtctcttcaa
     2041 ggactgtccc cgctgtttct ggctcgagaa ggtccgttac atcaaacgtc cgcgtggcat
     2101 tttcccttcg ctgccgggcg gtatggaccg ggtcatcaaa ctgtactttg acgggtttcg
     2161 ttccaaagga accctccctc ccgaacttgc ctcgcgcgaa gagtttcggg gactgtgtct
     2221 ctataaggat ctcgcgcagc ttgaacgctg gcgcgactgg aagaccgggc tcgagtactt
     2281 cgagaacggg ggccggcttt tcggagcgct cgatgatctc ctggtggcgg gagatgtttg
     2341 cgtgcccttc gattacaaga ccaagggatt gccgaccagc gaagaggacg cgacgaagta
     2401 ctaccagact caactggatt gttacgcatt gatgctggaa gcgaacggca tgaagaccgc
     2461 cggctatggt tttcttcttt attattctcc cagggaagtg gaggagaacg gtcgcgtgat
     2521 atttgagatt cagccgatcc gtatcggaac ggatattgaa cgcgcgagat cgtacttccg
     2581 gaggtcggcc gccgtttgcg aaggcccggc gccggccgcg tccgcgaatt gtgaatactg
     2641 cggttggctc aataagttca ggaaataaaa catggcggcc ggccggggga tagaggaaag
     2701 ctttttaagc gccctgcgtt ccgcgctgga atcgaggagt caagagtgac ggaaaaaaca
     2761 gaatggaaag tgctggttga aaaacccgcg cctgaaaaat taaaaagtct cggcgtgatg
     2821 aactggccga tctggatgaa ggaggtttcg gtctttgact ggtcctatga caaaaccgag
     2881 gtctgctatt ttctcgaagg cgatgtcacc gtcacgatcc cgggcgggga ggtgacgatc
     2941 gggaagggtg atctcgtgac cttccccgaa ggccttcact gtacgtggcg gatcaaaaaa
     3001 gccgtcaaaa agcattacaa gttcggctga acgacaacct gaaccgtaat atgaacgcgt
     3061 ttttcatgaa aggacttgat taaatggtgc tgcctgtccc aaaaacgaac gcaatgctga
     3121 tctgtgacta tgtgatcacg gagcgcggca cgaacaaaaa aagcctgatc ggcgttttcg
     3181 aaaatatcgg ggcgatgaaa tttccgtgta tccacgcggc aatgtcaatc tacgtgaagc
     3241 tgaccgacgc ccagggtagt taccgggtcc gtctggacct ggtcgatctc ggtgaggaca
     3301 agaccatcgg gcagaacgag attccgaacg atgtccaggt cccgagcccg ctcgtttcgc
     3361 acgagcttgt ttttaacctc cgcgatatcc atttcacgca tccgggcgaa tacgagttca
     3421 ggattttcgc gaacgggtcc atttttggac agaaaaaatt cactgttgaa caggcgagac
     3481 gcaataatgc cgaaggagtt ccctgatgcc caagacagcc gtggtgatcc tcgcggaagg
     3541 gtttgaagag atcgaggcga tcggtccggt cgatgtcctc cgccgggcgg gggttcgtgc
     3601 gatccttgcc ggcgtggacg gactgaccgt caagagttca aggggggttc ctgttctggc
     3661 ggaaattctc ctgaaagacg tgaaaggcct tcccgacgcc gcgatcgttc ccggaggcct
     3721 gccgggggcg acccatctcg caaagtcggc ggtggtagga gaattcctga agaaaatgaa
     3781 ctcggacaag cggctgatcg cggcgatttg tgccgccccg tccaccgtgc tttctccgct
     3841 tggtattctg gacgggaaaa aggcgacgtg ctatcccggc tgcgagacgg attttgcccc
     3901 gggggtcagg cattccgcgg accgtgtcgt ggttgatggg catatcatca caagccaggg
     3961 accgggcacc gcgctcgagt tcgcgatcgc cgtcacggag gcgctcgcgg gcaaggaaat
     4021 ggccggtacg gtgcgcgcga agatgctcat cggcgcttga cgcgaatgac ggtttacgag
     4081 atcgagcaaa aataccgttt gaaagacctt gccgcggttc gtcgcggtct gagggcgctt
     4141 ggcgcgaaaa agatcgcttc cggacgggaa acgaacgaat tctttgacag gaacggagag
     4201 ctcaggaaac ggaagatcgc gatgcggctc cggcgttacg acgggcagtc gacgttgacg
     4261 ctgaagggtc cgaaactccg gtcccgattc accaaaagag tggagcttga aatgccggtc
     4321 gagcacagtc cgctccgttc gatccttttg ctctcgggct tcaggccggt catgaaatac
     4381 acgaagaatc gcgagcttta cggtctcggg aaagcccgtg tcacgatcga ccggctccgg
     4441 ggcttcggct gtttccttga gatcgaggga acggtgtctg tgatcgcgcg gatcgcttcc
     4501 gcgctcggtc tcggggacgg tgaccgcgaa ccgcgttctt atttgcagat gatgttcgga
     4561 tggaaacatt gaacgggagg gagatatgat catcgcgacg gaactcaggg tcgggaacgt
     4621 cttcaaatcc gacgggaaac ttttcaaggt catcacgcag gaagtgaaag ggacgggaaa
     4681 gttcggcaaa agcgttcact gcaaggtccg gagcatcgag gacgggaatg tggccgaaaa
     4741 aagtttccgc gcggaggaca agattgagga cgtgccggtt cgtatcgtca aaatgcaata
     4801 cctctaccgg gaaagcgatc agttcgtttt tatgaacatg gaaaattatg agcagttcaa
     4861 tatcacggcc agggcggtgg gacggcacga ggtgttcctg aaggagaatg aagcgatcga
     4921 ggtcctcttc ggggaagggc gggttctgag catcatgttc ccgaagatcg tggaattgaa
     4981 ggtcacaagc acccc
//
LOCUS       DAOOBX010000024         4714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_20532_length_4714_cov_2.731917, whole genome shotgun sequence.
ACCESSION   DAOOBX010000024 DAOOBX010000000
VERSION     DAOOBX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20532_length_4714_cov_2.731917"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4455
                     /locus_tag="PLY30_00755"
     CDS             <1..4455
                     /locus_tag="PLY30_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64659.1"
                     /translation="DDFLKRFELQSEQERRFDLLGQLFPSLSAEGRTRLSEQPAEAVT
                     ILRGLRDYNIIQVQLQSWTAKTLRVKGISAAALANLNVFNVWLAARNASIHAKGRSSK
                     DQSEMLRAYLAAAVVSSNKINPSIRSLIAVAVFHVSKQDTEPFSGVSELWSDVLAFAG
                     ARGILSNDPMLIAAQLRTIRDVLGSAEWAGISGKPAVSELIKLCVVQGESSATLKAAV
                     QLYASSADKFRIDSLDEAAALARRGVTLTSIEGLKKALAGHGFIVRNARELLEVGDVL
                     GVFDAQLKFDFMAGLDAVGALLAKDGTAWILKDVYGLPRHRFAGSEGDQVTVGYSDGA
                     WQIKVTAQNGIRHAIEMRGDRIDHKCTTDGEESLFRLVRTGDRVRFEEMPGIDGEVPG
                     LDLLNLDSVLEVSARPDAGRFVFSVDVIGRDGARESIFEAGAMQAVTDARGSAEVSLV
                     SNPVLSGQHKNASTRLLVRGRPVMIAEQFTDVDGSTKTREQELMLPPNVRVGTVQGSV
                     AVIEILDFAKKAAVGSFSVDLLTGKVVDEGSLLKGLMSIASYQDLEPEMAPVDRDLRI
                     LEHSTGYKLLLGQTVYAELAKDPEKGKAFISWWNRNAGGLSEPVLVEAVQAEFAGLEA
                     ILQGFKALFDETDPKMLAYYASKSDLQVAQRRLRDARFALDSEEAIALMDALHAAFAA
                     SDRASAEAVMKDSPDRIVRRLMAKIDDPAMRKQVNRVLSMMAASKEKQGLEVSLKDLR
                     DIFQKAKLDAELLKALDPFIAFGDFVSAAVGNNEILRAATTSLGGRADPKKLGYYLGY
                     LASHAQAWDLLQYKRREHFIKRSETEKNGLWTMYEEAQRTGDYTVVAEAFADQFMNLN
                     RAQLLAPQRGAFIKTLAELFASDGYDRVTFSLQAGSEVAPLRVTEARVKELVEQGKSR
                     EEAQAEANREWRGSRGYSDPKVPTLIFHGDAAKKLGRYWYFVGKEWAQTTGAESFVDS
                     DFDTGKAGVAEMFAHTGLVVIDDRWLEQSYFHEEQHIVFQRPYVVRAKGDPRVEEVYN
                     YFAQILSRTIDLETGRAYDVQMIYNKMKSYAKNQGFDYDVMKGAFDAILYLYQNTDEA
                     GKVTIMNLIRHTQKVEELLAWHAVQPMDIKKEFDAIYEQAFEPRTELKEEVPEPAEVL
                     PEADLGVAGLAADVPATPEAFVDAVKANLLAAAPGAGIELSEAAKRLQMMTPVQDPRL
                     QGKLGFYTDPVGADAFGIMALNSQKAEAFYQKIQGKTVLTLEERGELQDIMAELLVAL
                     AHENEHAKGRAEVRAYQKSIDVAKLVKMPKFDLLYILLRQARAEETELKRVQDALQLV
                     DQNGMMVRRDALDVLSNLNNSRTVAIMSPELAAINGVESVGDVAGLGKYLKDTKYTAS
                     ANHTVFFIEDAKLKMPGMPEALERLRKAGFPIFVYSESDIGGGADRVFYLGQFAAWVG
                     VEEFRRLMPEMYHENRNGYFSVTAYLSNALIRTLLNEIMAEQQVAQAA"
BASE COUNT         1018 a         1179 c         1480 g         1037 t
ORIGIN      
        1 gatgacttcc tgaagcgttt tgaactacag agtgagcagg aacggcgctt tgatctgctc
       61 ggccagttgt tcccgtcgct cagcgcggaa ggccgcaccc gcctttcgga gcagccggcg
      121 gaagcggtca ccatcctgcg tggtctccgc gattacaata tcatccaagt ccagctccaa
      181 agctggaccg cgaagacgct cagggtgaaa gggatcagcg ctgctgcttt ggcgaacctg
      241 aacgtgttca atgtctggct tgccgccagg aatgcttcga tccacgctaa gggcaggtcc
      301 tcgaaagacc aatccgaaat gctcagagcc tatctggccg cggcggtcgt ttcttccaac
      361 aagatcaatc catcgatccg ttcgctgatc gctgtggcgg tgtttcacgt ttcaaaacag
      421 gacacggaac cgttcagcgg cgtctccgag ctctggagcg atgttctcgc gttcgcagga
      481 gctcgaggca tcttgagcaa cgatccgatg ttgatcgccg cgcagttaag aacgatccgt
      541 gatgttctcg gctctgcgga gtgggcgggg atcagtggga aacccgcggt ctcagaactc
      601 atcaaacttt gcgtggtcca gggggaatca tccgcgacgc tcaaggcggc agtacagctt
      661 tatgcgtcct cggcggataa gttccgcatc gattccctgg atgaagcggc ggctcttgcc
      721 cgtcgcggcg tgacgctgac ctcgatcgag ggtctgaaaa aggctcttgc gggccatgga
      781 tttattgtcc ggaacgcccg cgaattgctt gaggtgggag atgttctcgg cgtttttgac
      841 gcgcagctga aattcgattt tatggccggt ctcgatgcgg tcggagcgct tcttgccaaa
      901 gacggaacgg cgtggatcct taaggatgtt tacgggttgc cgcgccaccg gtttgccggt
      961 tctgaagggg atcaggtgac ggtagggtac tcggacggtg cctggcagat caaggtaacg
     1021 gctcaaaacg ggatccgcca cgcgatcgag atgcgcggcg atcgtatcga tcacaaatgc
     1081 acgacggacg gcgaggaatc gctgttccgg ttggtccgga ccggcgatcg ggtccggttt
     1141 gaggaaatgc ccggtattga tggcgaagtc cctggacttg atcttttgaa cctcgacagt
     1201 gtccttgagg tcagtgcccg tccggatgcg gggcggtttg ttttctccgt ggatgtgatc
     1261 gggcgggacg gtgcccgcga gagcattttt gaggccggag ccatgcaggc ggtcacggac
     1321 gcgcgcggga gcgcggaggt gtcccttgtt tccaatcccg tgctgagcgg gcagcataag
     1381 aacgcttcga cccgtttgct cgtgcgggga cgtccggtca tgatcgctga acaattcacc
     1441 gatgtggacg gatcgacgaa aacgagagag caagagctga tgctccctcc gaacgttcgc
     1501 gtcggtacgg tccaggggag cgtcgcggtt attgagatcc tggattttgc caaaaaggca
     1561 gctgtcggct cattctcggt ggatctcctg acagggaaag tggttgacga gggatctttg
     1621 cttaaaggtc ttatgtcgat cgcttcctat caggaccttg aacccgagat ggcgcccgtg
     1681 gacagggacc ttcggattct tgagcattcg acgggttata agctgcttct cggacaaacc
     1741 gtttatgcgg agcttgcgaa agatccggag aaggggaagg cgttcatttc atggtggaat
     1801 cgtaacgctg gcggactttc ggagcctgtg cttgttgagg cggttcaggc cgagtttgcc
     1861 ggtctcgaag cgatccttca gggctttaaa gcgctctttg atgaaacgga tccgaaaatg
     1921 ctcgcttatt atgcttcaaa gagcgacttg caagtcgcgc aacgacggct ccgcgacgcg
     1981 agatttgccc ttgatagcga ggaggcgatt gcgttgatgg atgcccttca cgccgctttt
     2041 gccgcctccg atcgcgcttc cgcggaagcg gtcatgaagg attctccgga ccgcattgtg
     2101 cggcgtctga tggctaagat cgatgatccc gcaatgagga aacaggttaa tagggtgctt
     2161 tcgatgatgg ccgctagtaa agaaaagcaa gggcttgaag tgagcttgaa agatctccgc
     2221 gatatcttcc agaaagcgaa gctcgatgcc gaactattaa aagccctcga tcctttcatc
     2281 gcgttcggtg atttcgtttc ggccgcggtc ggaaataatg agatcctccg ggctgcgacg
     2341 acgtctcttg gcgggagagc tgacccgaaa aagctgggtt attatctcgg ctatctcgcg
     2401 tctcacgcgc aagcgtggga ccttctgcag tacaaacgga gagagcattt tatcaagcgg
     2461 agtgaaaccg aaaagaacgg actctggacg atgtatgaag aagcccagcg taccggcgat
     2521 tatacggtgg tagcggaggc gttcgcggat cagttcatga acctcaaccg cgcgcagttg
     2581 ctcgctcctc agcgcggagc cttcatcaag acgctcgccg aattgttcgc gagcgatggt
     2641 tacgatcgcg tgacgttcag tctccaggcc ggctctgagg tggcaccgct gcgtgtgaca
     2701 gaagcccgtg tgaaggaact tgtggaacag gggaagagcc gcgaagaagc gcaggcggag
     2761 gccaatcggg agtggcgagg atcgcggggc tatagtgatc cgaaggtccc gacgcttatc
     2821 ttccacggtg acgccgcgaa gaagctgggc cgttactggt attttgtcgg aaaagaatgg
     2881 gcgcaaacga ccggcgcgga gtcgttcgtt gatagcgatt ttgataccgg aaaagctggc
     2941 gtggctgaaa tgttcgcgca taccgggctt gtcgtgatcg atgatcggtg gctcgagcag
     3001 tcgtatttcc atgaagaaca gcacattgtg ttccagcggc cttatgtcgt tcgtgccaaa
     3061 ggagatcctc gcgttgaaga agtgtataac tattttgccc agatattgtc ccgcacgatc
     3121 gatcttgaaa cggggcgcgc ctatgacgtt caaatgatct acaacaagat gaaatcctac
     3181 gcgaagaacc aaggctttga ttatgacgtc atgaaaggcg cttttgacgc tatcctgtat
     3241 ctttatcaga acaccgatga ggccggtaaa gtcacgatca tgaacctcat tcggcatacc
     3301 cagaaagtgg aagagcttct tgcctggcat gctgtccagc ccatggacat caaaaaagaa
     3361 tttgacgcta tctatgagca ggcctttgag cctcggacag agctgaagga agaagtccct
     3421 gaaccggcgg aagtgctgcc ggaagcggac ttgggtgtcg cggggctcgc ggcggatgtg
     3481 ccggcgacgc cggaggcttt cgtggacgcg gtaaaggcga atctcctggc ggcggcgccg
     3541 ggtgccggca ttgaactttc cgaggccgcg aagcggcttc aaatgatgac gccggtccag
     3601 gatccgagat tgcagggcaa gctcggtttc tacacggatc ctgtcggcgc ggacgcgttc
     3661 ggcattatgg ccctgaacag ccaaaaagca gaggccttct atcagaagat ccaggggaaa
     3721 acagtgctga ccctcgaaga gcgcggcgag cttcaggata tcatggcgga gctcctggtc
     3781 gctctcgctc acgagaatga gcacgcgaag ggccgcgccg aggtccgcgc ttaccagaag
     3841 tcgatcgatg tggcgaagct cgtgaagatg ccgaaattcg atcttctgta tatcctcctg
     3901 aggcaggcaa gggccgagga gacggaattg aagcgcgttc aagatgccct ccagcttgtg
     3961 gaccagaacg gtatgatggt gcgccgcgat gcgctcgatg tcctgagcaa cctgaataat
     4021 tccaggacgg tggcgatcat gagccccgaa ctcgcggcga tcaacggggt cgagtcggtc
     4081 ggggatgtcg cggggcttgg aaaatacctc aaggatacga aatataccgc gagcgcgaat
     4141 cacaccgtgt tctttatcga ggacgcgaag ctgaagatgc cgggaatgcc ggaagcgctg
     4201 gagcgtttgc ggaaggccgg gttcccgatc ttcgtttaca gtgagagcga tatcgggggc
     4261 ggcgcggatc gcgtcttcta tctcgggcaa ttcgcggcgt gggtgggcgt tgaagagttc
     4321 aggagactga tgcctgaaat gtatcacgaa aaccgcaacg gctatttctc ggtgaccgcg
     4381 tatttgagca acgcgctgat ccggaccttg ttgaacgaaa tcatggccga acagcaggtc
     4441 gcccaggcgg cttaagaaac ggcaattccg acctattttg aattagtaga tggcgtgttc
     4501 ttgtagggtc ttgttgtctt ggggacgttt tggggcttcg gtgaagggct cctgccttag
     4561 gttcatccta ttctggcaac ccaaaagaaa ccttttgtca gaatatataa cgtccgataa
     4621 tgtacgttat gtaaagtaac accaagggag cgtggcgccg ttgcgaatag gaccggatcc
     4681 ccgagtgttt tagtgtttgg gtatgatggg cctg
//
LOCUS       DAOOBX010000025         4699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_20618_length_4699_cov_3.906977, whole genome shotgun sequence.
ACCESSION   DAOOBX010000025 DAOOBX010000000
VERSION     DAOOBX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20618_length_4699_cov_3.906977"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="PLY30_00760"
     CDS             complement(<1..1794)
                     /locus_tag="PLY30_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPW64660.1"
                     /translation="MNDQKLAPISLTPTQKKAVQHLSTDLIVSAGAGTGKTRVLVERI
                     LHILKNGKAGIGELLILTFTDKAANEIKTRLSAGLRNLGLEQIRRELEQAAIGTFHAF
                     AARLLKEHPLEARVDPDFRVLESEQADLLQDEAATRVLETTFRDNDPSFDAVRVYGEN
                     AIRSGFYQVFAAARHEGRTLTEFFSESREKKEAVLKEAGSRLLREAEAQAAKLGDDFD
                     KASWVRFKDQPFWDWQTLGDFREWRKQYSCKHKDGWKEWRNLADALAAVKIEPLAEPW
                     AEKFEALALKFERTYEAAKKEENRLDFDDLQSRAVALFADSRPACQKMRERYRRRFKF
                     ILVDEFQDTNYLQMRFIGLLSSGSNVFTVGDYKQSIYGFRGAEPRIFLELDEKFRSGK
                     AGVRIPLVENFRSGARLLEKTNAIFRELWREDRDFPFEELEARAEEGPGPVPAEPVEM
                     LVTNLGEDEGGKEHAKIREAIAVAARIRSLREREGVPYGNIAVLFRGMKQSGIYEHAM
                     KVFDIPYFIMSGRGFYHQPEIRDIVSYLTHLEKPLLDIPLAAALRSPLFHITDSTLFW
                     LSKRAKSESKTAPLRRALKEACNIHSITEDQQ"
     gene            1896..2330
                     /locus_tag="PLY30_00765"
     CDS             1896..2330
                     /locus_tag="PLY30_00765"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPW64661.1"
                     /translation="MENNKRKFYRHPIKVPIRLTQIKATVAAPSKSEDLSEGGLSFYW
                     PDALPEGAHLELAIPVEKHLFRMEAHVAYSKRDNAMGLYKTGVCFKDPTSAFRAKLAE
                     EIIKIRAYREKMETLRGQAFSEEEAAARWISRNAEAFAKLFG"
     gene            complement(2439..3134)
                     /locus_tag="PLY30_00770"
     CDS             complement(2439..3134)
                     /locus_tag="PLY30_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW64662.1"
                     /translation="MVYQPIIDVATWDVIGHEALARSEKETPVEMFHKSYKKGTIVSS
                     DFYCMEAAVKALPTFPEGQYLFLNIEPPTLKEAFAAGKEGEMFLKHFRAYSDRIVFEL
                     TESVKRSDFEFIKKGVRFIRALGYRLALDDLTEIGAKTLQLASLRPEFLKIDLHLIRG
                     IANNNLNQQIVREMVHLGRMNGSEMIAEGVETREDCDFVRNMGIRYSQGYYFAKPGRN
                     PVSFLQPPKDTQV"
     gene            complement(3268..3444)
                     /locus_tag="PLY30_00775"
     CDS             complement(3268..3444)
                     /locus_tag="PLY30_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HPW64663.1"
                     /translation="MGRPSRLKVLPVAVKLLPGEMAEILPPGKKVVPEKLVRSGFGFK
                     CFSPDQALKAIFPE"
     gene            complement(3523..3978)
                     /locus_tag="PLY30_00780"
     CDS             complement(3523..3978)
                     /locus_tag="PLY30_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPW64664.1"
                     /translation="MKLAIRTAAQGMKKNDGGPFGACIVRNGRVIATAHNTVLLKKDA
                     TCHAEVNAIREASRKLRRFDLSDCVIYSTTEPCPMCFSAIHWARIPLVIWGTAIADVA
                     RLGFNELSLPNSRMKRYGKSPVKSRGKFLLKECRALLEKWERMDSKKIY"
     gene            complement(4017..>4699)
                     /locus_tag="PLY30_00785"
     CDS             complement(4017..>4699)
                     /locus_tag="PLY30_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPW64665.1"
                     /translation="IDRGTSKAAEVFWTLDPIDGTKGYLRGGQYVTALALIRNGKVEF
                     SALGCPELALPGHEKPGAGVILLAIRERGCWASPLCQDGPWERLRVSSCRDITRARIL
                     DSCDPGHKNAEKNAFIRNTLGIVAEPVAMDSQAKHATLASGGAEIFSRTPPRRDPAYC
                     EKIWDVAPGAFVIEEAGGCVTDLSGDPIDYGTGNILKNNHGFVATNGVFHGQMLEAIR
                     EAVAALRE"
BASE COUNT          999 a         1373 c         1265 g         1062 t
ORIGIN      
        1 ctgttggtct tccgtgatgc tgtgaatgtt acaggcttct ttcagggcgc ggcgaagcgg
       61 cgccgtttta ctttcggatt tcgcgcgctt cgaaagccag aaaagagtgc tgtccgttat
      121 atggaaaagg ggcgagcgga gcgccgccgc gagagggata tccaggagcg gcttttcgag
      181 atgcgtgaga tagctcacga tgtcccggat ttcgggctgg tgatagaagc cgcggccgga
      241 catgatgaaa tacgggatgt cgaagacctt catcgcgtgt tcatagattc cgctttgttt
      301 catcccgcgg aaaagcaccg cgatgttccc gtaagggacg ccctctcttt cgcggaggct
      361 cctgatccgc gccgcgaccg cgatcgcctc gcggatcttc gcgtgttctt tccctccttc
      421 atcttccccg aggttcgtca cgagcatctc cacgggctcc gcgggcaccg gtcccggccc
      481 ttcttccgcc ctggcctcca gctcctcgaa agggaaatcc cggtcctccc tccaaagctc
      541 gcgaaagatc gcgtttgttt tttcgaggag gcgggcaccg cttctgaagt tctcgacaag
      601 cgggattctc acacccgctt ttccggaacg gaatttttcg tcgagttcaa ggaagatccg
      661 cggctcagcg ccccgaaaac cgtagatcga ctgtttgtaa tcccctaccg tgaacacatt
      721 cgatcccgag gagagaagtc cgataaatcg catctgcagg taattcgtat cctggaactc
      781 atcgaccagg atgaacttga acctccggcg gtagcgttcc cgcatcttct ggcacgcggg
      841 acgggaatcc gcaaaaaggg cgacagcacg gctctggaga tcgtcaaaat caaggcggtt
      901 ctcttctttc ttcgccgcct cataggtcct ctcgaattta agggccagcg cttcaaattt
      961 ctcggcccac ggttcggcga gcggttcgat cttcacggcc gccagcgcgt ccgcgagatt
     1021 gcgccactct ttccatccgt ctttgtgttt gcaggaatat tgttttctcc actcacggaa
     1081 atcaccgagg gtctgccagt cccaaaaggg ctgatccttg aaacggaccc atgaagcctt
     1141 gtcgaaatcg tcaccgagtt tcgccgcctg cgcctcagcc tcgcggagaa gccttgatcc
     1201 ggcttccttc aaaaccgctt cctttttttc gcggctctcg gaaaagaatt ccgtcagcgt
     1261 tctcccttca tggcgtgcgg cggcaaacac ctgatagaaa ccggaacgga tggcgttctc
     1321 gccgtagacg cgcaccgcat caaaggaagg atcattgtcg cggaaggtcg tctcaaggac
     1381 ccgggtcgcc gcctcatcct gaagaagatc ggcctgctcg gattccagca cccggaaatc
     1441 gggatcgacc cgcgcttcga gcggatgctc cttcaaaagc cttgccgcga aagcgtgaaa
     1501 cgttccgatc gcggcctgct cgagttcacg gcgtatttgc tcgaggccga gattccgaag
     1561 tcccgcggaa agtcgggtct tgatctcatt ggccgcttta tcggtgaagg taaggatcag
     1621 cagctcgccg atcccggctt tgccgttctt gaggatatgg aggatgcgtt caaccaggac
     1681 tctcgtttta ccggtccctg cgccggctga gacaataagg tccgtggaca ggtgttggac
     1741 cgcttttttt tgtgtcggcg tcagtgatat gggtgctagt ttttgatcgt tcatgtttcc
     1801 ataaatacca tgggtgcaag atgcgtgggt ccgtattata caaaaaaatc aaccgtccta
     1861 tggaaaaaat ccctccggaa gttatacttc aaccgatgga gaacaataaa agaaaatttt
     1921 acagacaccc gatcaaagta ccgattcggc tgacacagat caaagcgacg gtcgcggcac
     1981 cttcgaagtc tgaggatctc tcggaaggcg ggctttcgtt ctactggccc gatgcccttc
     2041 ccgagggcgc tcacctcgaa ctggcgatcc cggttgaaaa acacctgttc aggatggaag
     2101 cccatgtcgc ttactccaaa agggacaatg cgatggggct ttacaagacc ggcgtctgct
     2161 tcaaggaccc cacaagcgct ttccgcgcga aactcgccga agaaatcatc aagattcgcg
     2221 cgtaccggga aaaaatggag acactgcggg gacaggcgtt ttccgaagaa gaagccgccg
     2281 cccggtggat cagccgcaac gccgaggctt tcgcgaagct tttcggttaa tggggccgtc
     2341 acacccgggt cgtcagagtc gttgagagat ttttgatccc accctcatga tccgaaaaat
     2401 ttttgggcag gaatcttccc caagggcaca ggctgcgatc agacctgagt gtctttggga
     2461 ggctgcagga aagaaaccgg gttccggccg ggttttgcga aataatatcc ctgactgtag
     2521 cggatcccca tattgcggac aaagtcacag tcttccctgg tctccacgcc ttcggcgatc
     2581 atttccgaac cgttcatcct gccgaggtgg accatttcgc ggacaatctg ctggttgaga
     2641 ttattgttcg cgatcccccg gatcagatgg aggtcgatct tcaggaactc cggcctgagg
     2701 gaagcaagct gaagggtctt cgcgccgatc tccgtcaggt cgtccagcgc aagccggtaa
     2761 ccgagggcac ggatgaacct gacgcctttt ttgatgaact caaaatccga ccgtttgacg
     2821 ctttccgtca gttcaaaaac gatcctgtcg gaatatgccc tgaagtgctt cagaaacatt
     2881 tcaccttctt tgccggcggc gaaggcctct ttgagcgtcg gcggttcgat attgagaaag
     2941 aggtattgcc cctccgggaa cgtggggagc gctttgacgg ccgcttccat gcaataaaaa
     3001 tcagaagaaa cgatcgtccc ttttttatag gacttgtgga acatctcgac cggcgtttct
     3061 ttttcgctac gggcaagcgc ttcatggccg atcacgtccc aggtcgcgac atcgatgatg
     3121 ggctggtaga caatacacgc ccgaaggcat gtcgggcttg tgttttttga ataaattcac
     3181 gaaaaaatcc acgtcgttac tcctcgatta aaaaaacgca gtaagtgtac acgatgtctg
     3241 gtggatttac cagaccgcga gcccttctca ttccgggaaa atcgccttca gggcctgatc
     3301 gggggagaaa catttgaatc cgaaaccgga gcgcacgagt ttttcgggaa caaccttctt
     3361 ccccggcgga agtatctccg ccatctcgcc ggggagcagt ttgaccgcga ccggaaggac
     3421 cttcagccgg gatggtcgtc ccatggcgcg gccacgggca ttttacagaa aacctccatc
     3481 ccctgaggcg ccggacgcac gatcgctcta atcatcttta ccttaataga tctttttact
     3541 gtccatccgc tcccattttt ccaaaagcgc ccggcattcc ttcaaaagaa acttcccccg
     3601 gctcttgacg ggactttttc cataccgttt catgcgggaa ttcggaagag acaattcatt
     3661 gaaaccgaga cgggcgacgt cagcgatcgc cgtaccccag atcacgagcg ggatacgcgc
     3721 ccagtggatc gcggaaaaac acatcggaca gggttcggtc gtggagtaga tcacgcagtc
     3781 gctcagatca aaccggcgga gcttacgcga ggcttcgcgg atcgcgttga cctcggcatg
     3841 gcaggtcgcg tcctttttca gcagaacagt gttatgggca gtcgcaatga ccctgccgtt
     3901 acggacaata caggcgccaa aaggccctcc gtcgtttttt ttcattcctt gcgcggctgt
     3961 ccggatcgca agtttcatcc attttgacaa cggttgatgt gtcatcttct tccctcctat
     4021 tcgcgcaaag cggcaaccgc ctcacggatc gcctcgagca tttgcccgtg aaaaacgccg
     4081 ttcgttgcca cgaagccgtg gttgttcttc aggatatttc ccgtgccgta atcgatggga
     4141 tccccactca ggtccgtcac gcatcctccc gcttcttcga tcacgaacgc ccccggcgcc
     4201 acatcccaga tcttttcgca gtaggccgga tcgcgacgcg gcggagtccg ggaaaagatc
     4261 tccgcgcctc ccgatgcgag ggtcgcatgc ttcgcctggc tgtccatcgc gaccggttcg
     4321 gcgacgatcc cgagcgtgtt cctgatgaaa gcgttctttt ccgcgttctt gtgccccggg
     4381 tcacaagaat ccaggatgcg tgcccgtgtg atatcacggc aggaagaaac ccgcagccgt
     4441 tcccatggac cgtcctgaca taaaggagaa gcccagcaac cccgttcccg gatcgcgagc
     4501 aggatcacgc ccgccccagg cttttcatga ccgggaagag cgagttccgg acagcccagc
     4561 gcggagaact ccactttgcc gttccggatc agcgcgagcg ccgtcacata ttgccctccg
     4621 cgcaaataac ctttcgtgcc gtcgatcggg tccagcgtcc agaaaacctc cgcggccttc
     4681 gaagtccccc ggtcgatcc
//
LOCUS       DAOOBX010000026         4664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_20795_length_4664_cov_4.353656, whole genome shotgun sequence.
ACCESSION   DAOOBX010000026 DAOOBX010000000
VERSION     DAOOBX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20795_length_4664_cov_4.353656"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="PLY30_00790"
     CDS             <1..2010
                     /locus_tag="PLY30_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPW64666.1"
                     /translation="VQAGETELTVACSEVVANAMKDWAIEKGATHYTHWFQPLTGRTA
                     EKHDSFISPTDDGSVIMEFSGKQLTQGEPDASSFPSGGLRSTFEARGYTAWDCTSPAF
                     LKTDASGNVTLCIPTAFCSYTSEALDEKTPLLRSMEAVSKQAIRVVKALGNKTSKRVT
                     STVGPEQEYFLIDKTYYDKRLDLIVSGRTLFGAPAPKGQELEDQYFGAIKDRIAAFMR
                     DLDVELWKMGISSKTKHNEVAPAQFEMAPIFSTANIAADHNQLVMETMQKVALRHGLV
                     CLLHEKPFAGMNGSGKHNNWSLSTDDGINLFEPGRTPAENQQFLIFISAVIMAVDRHA
                     GKLRASCGNPGNDRRLGTNEAPPTIISIFMGEEFTEILRGIAQGKKVVNRGQNFLQVG
                     VDSLPQLPKDNTDRNRTSPFAFTGNKFEFRMVGSSASISGPNTVINTIVADALAEIAT
                     RLEKAGKGKLNREAAAIVRDAMKKHGRVIFNGNNYSDAWIAEARKRGLPNVRTGVNAF
                     AAMASPEAVKLFEKHKVLSGGELHARHEIYLDGYVKHVNIEAQASVQMAKKLYIPSVI
                     KFTDCLAGTIGRLRAVTASARVQKELLASVTELLESANDKLATLETAIVKAHHIEGLE
                     ARAFAFRDKVVTAMNALRKDIDALENIVPSELWPVPGYADMLFKL"
     gene            2236..2565
                     /locus_tag="PLY30_00795"
     CDS             2236..2565
                     /locus_tag="PLY30_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HPW64667.1"
                     /translation="MFVLLTSSTVLQATEIEREGTMCRKLQRGFLNVALSPIELSNEL
                     AKEKLTNTLPPSWFAGLGRGAWFTLGRALTGVYEMVTFPIPYPANYRPILKPEFAWQH
                     LPSADEN"
     gene            complement(2753..3895)
                     /gene="lpxB"
                     /locus_tag="PLY30_00800"
     CDS             complement(2753..3895)
                     /gene="lpxB"
                     /locus_tag="PLY30_00800"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPW64668.1"
                     /translation="MPKKIFIIACEASADRHGAHLVAELRKFLPDTEFCGHGGSKMAA
                     AGVRVFHDMTKISALGFGDVIRNYLTYRKIFHDTIALIGKERPDILVTVDAPAFNLRL
                     AKKVSKLVPIFYFISPQLWAWGKRRIHIIKKHVREMLVIFPFEKDFYEKEGVAVKFVG
                     HPLLDALPGLPDSSTLRSRFGIPSRTKVIGLFSGSREKEVKRILPVMLQSAALIRKEM
                     SAVSFIVSKSPNVRPQVYDEILKASSLPVLTPPGSFYELVKCADFALVTSGTATLETA
                     LLDTPFFLLYKTGAVTYFLGKRLIKVPYLGIVNLLANNRVVPEFIQQDARPETIARET
                     RVFLNDPELQARMKEEFRRVRESLGEKGASRRAAKEICDFLATRPN"
     gene            complement(3897..>4664)
                     /locus_tag="PLY30_00805"
     CDS             complement(3897..>4664)
                     /locus_tag="PLY30_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW64669.1"
                     /translation="DLLGKVDAVSIATPTSTHYTVAREFLEKGVHVLVEKPITLRLRE
                     ADELIRISRKRRCTLQVGHIERHNPAFKQVREIAKKIRFLEIHRLGPFTGRINDCGVV
                     LDLMIHDLDIVLGLVDSPFEHFDAIGVNVLTPFEDIANVRLKFRNGAIANITASRLTF
                     EKQRKIRIFQEDAYISLDYGAQTGKIIRKSGNEILQEDLLIEKAEPLKEELKFFLEGI
                     RTGRGPGKPDIAARNALAIALKIAEVIQKNHPKPGAA"
BASE COUNT          976 a         1305 c         1422 g          961 t
ORIGIN      
        1 gttcaggccg gcgagaccga gttgacggtc gcctgttccg aagtcgtggc gaacgccatg
       61 aaggactggg cgatcgaaaa aggcgcaacc cactacacgc actggttcca gccgctcacg
      121 ggccgcaccg cggaaaaaca cgattctttc atctctccta cggatgacgg cagcgtaatc
      181 atggagttct ccggcaagca gcttacgcag ggggaaccgg acgcctcctc attcccgagc
      241 ggcggtctcc gctctacctt cgaggcgcgc ggttacacgg cctgggattg tacgtcaccc
      301 gcgttcctga agacggacgc ttccggaaat gtgacgcttt gcatcccgac ggcgttctgt
      361 tcctatacga gcgaggcgct ggacgagaag accccgttgc tccgttccat ggaggctgtt
      421 tccaagcagg ccatccgggt ggtgaaggct ctcggcaaca agacctcgaa acgggtgact
      481 tcaaccgtcg gtcccgagca ggaatacttc ctgatcgaca agacctacta cgacaagcgc
      541 ctcgatctga tcgtctccgg ccgtacgctt ttcggagccc cggccccgaa agggcaggaa
      601 cttgaagacc agtatttcgg agcgatcaag gatcgtattg ccgcctttat gcgggacctg
      661 gacgtcgaac tctggaagat ggggatctcc tccaagacaa agcacaacga ggtcgcgccc
      721 gcgcagttcg aaatggcgcc gatcttttcg acggccaata tcgcggcgga tcacaaccag
      781 cttgtgatgg agaccatgca gaaggtggcg ctccgccacg ggctcgtttg cctcctgcac
      841 gagaaaccgt tcgcgggcat gaacggctcc ggcaaacaca acaactggtc cctttcgacc
      901 gatgacggca tcaatctgtt tgaacccggg aggacccccg cggagaacca gcagttcctg
      961 atcttcatca gcgcggtgat catggccgtc gaccgtcacg ccgggaaact gcgcgccagc
     1021 tgcgggaacc ccggcaacga tcgtcgtctc ggcacgaacg aagcgcctcc gacgatcatt
     1081 tcgatcttca tgggcgaaga gttcaccgag attctccggg gcatcgccca ggggaaaaag
     1141 gttgtgaacc gcgggcagaa tttcctgcag gtcggcgtcg attccctgcc gcagttgccg
     1201 aaggacaaca ccgaccggaa ccgcacgtcg cctttcgcct ttacgggcaa taaattcgaa
     1261 ttccgcatgg tgggttcttc ggcgtcgatc tcgggaccga acaccgtgat caatacgatt
     1321 gtggcggacg ccctcgccga gatcgcgacc cgtctcgaaa aagcggggaa ggggaagttg
     1381 aacagggaag cggccgcgat cgtccgggac gcgatgaaaa aacacggccg ggtcattttc
     1441 aacggtaaca attattcgga cgcgtggatc gcggaggcca ggaaacgagg tctccctaac
     1501 gtccggacgg gtgtcaacgc gttcgcggcg atggcttcgc cggaagccgt gaagttgttc
     1561 gaaaaacaca aggtgctatc gggaggcgag ctccacgccc gccacgagat ctatcttgat
     1621 gggtacgtga aacacgtgaa tatcgaggcc caggcctcgg tccagatggc gaagaaactt
     1681 tatatcccgt ccgtgatcaa gttcacggac tgcctggcgg gcaccatcgg ccggctcaga
     1741 gccgtgacgg cttccgccag ggtccagaag gaacttctcg caagcgtgac cgagcttctc
     1801 gaatccgcga acgacaaact cgcgacgctc gaaactgcga tcgtgaaagc gcatcatatc
     1861 gaggggcttg aggcgagggc atttgctttt cgcgacaagg tcgtgaccgc catgaacgcg
     1921 ctccgcaagg atatcgacgc cctggagaac atcgtcccct cggaactctg gccggtgccg
     1981 gggtacgcgg acatgctctt taaactgtaa tccgggaaaa atccctggtt caaaagaaaa
     2041 gttgcctgac tccgttggca aatgataaat tactccgtca tgagcctcat gggaggcgga
     2101 aggcgtttca ggaacgtttt cgggatgtct tccgggttgc gggatgcttt taaacggata
     2161 tttttgtcga gggaaaacac gatgacttta ccggtctctg gcggggcgag gaacacattg
     2221 aagagccttg ccgttatgtt cgttctcctg acgagctcga cggtcctgca ggccacggag
     2281 atcgaaaggg aagggacgat gtgccgcaag ctccagcgcg gtttcctgaa cgtcgcgctt
     2341 tccccgatcg agctttccaa tgaactcgcc aaagaaaaac tgaccaacac ccttcccccg
     2401 agctggttcg cgggactcgg ccgcggagcg tggttcacgt tggggcgggc cctgacgggc
     2461 gtgtatgaaa tggtgacctt cccgatccct tacccggcga attaccggcc cattctcaag
     2521 cccgaattcg cgtggcagca tttaccatcc gcggatgaga attaaataga ggtaaacgca
     2581 gtcctgatct ccggtttccc ccggggaaaa gttgtccttc cttccatccg tcccgattgc
     2641 gtcacggatc gtcggggcgg tctcaaaggt cttacagcgg tcctcacggt agaatcgtca
     2701 ggttcgcgaa gaccggatgg cgagcgcggt agagaagcgg aaaatccgga agctagttcg
     2761 gccgggtggc cagaaaatcg cagatttcct tcgcggcgcg acggctcgcg cctttttctc
     2821 ccagagattc cctgactcgt cggaactctt ctttcatcct ggcttgaagc tccggatcgt
     2881 tcagaaagac gcgcgtttcg cgggcgatcg tttcggggcg ggcgtcctgc tgtataaatt
     2941 ccgggacgac ccggttgttc gcgagaaggt tcacaatgcc gaggtagggg accttgatca
     3001 gtcgtttccc caggaaatag gtgaccgctc ccgttttata gagcaggaaa aaaggcgtgt
     3061 cgagaagcgc cgtttcgagt gtcgccgttc cggaggtcac gagcgcgaag tccgcgcatt
     3121 tgacgagttc ataaaaagac ccgggagggg tgaggaccgg caggctcgaa gccttcagga
     3181 tctcatcgta cacctgcggc cgcacattcg gggactttga cacgatgaaa gagacggccg
     3241 acatctcttt tcggatgagc gccgccgact gcagcatgac gggcaggatc cgcttcactt
     3301 ccttctccct cgaaccggaa aaaagtccga tgaccttcgt ccgggaaggg atcccgaagc
     3361 gggaccggag cgttgaactg tccggaagtc cgggaagcgc gtcgaggagg gggtgcccga
     3421 caaatttgac ggcgacgcct tctttttcgt agaaatcctt ttcgaaagga aagatcacga
     3481 gcatttcacg gacatgtttt ttgatgatat ggatgcggcg tttgccccag gcccacagct
     3541 gcggcgagat gaaatagaag atcggaacga gcttcgagac ttttttcgcg agccggaggt
     3601 tgaatgccgg ggcatcgacc gtgacgagga tgtccggcct ctccttcccg atcagcgcga
     3661 tcgtgtcatg aaagatcttc cggtaggtga ggtaattcct gatgacatcg ccgaagccga
     3721 gcgcggagat ttttgtcatg tcgtggaaga cccgcacgcc cgcggcggcc attttgctcc
     3781 cgccgtggcc gcagaactcc gtgtccggga ggaatttacg gagctccgcc acgagatgag
     3841 cgccgtgacg gtcggcggag gcctcgcagg cgatgataaa gatctttttc ggcatttcag
     3901 gcggcgccgg gcttgggatg gtttttctgg atgacttcgg cgatcttgag ggcgatcgcg
     3961 agggcgttgc gggccgcgat atcgggtttc ccggggccgc gccccgtgcg gatgccttcc
     4021 aggaaaaact tcagttcttc cttgaggggc tcggcctttt cgatcaagag atcttcctgg
     4081 aggatttcgt tgccgctctt gcggatgatc ttcccggttt gtgccccgta atccagggag
     4141 atataggcgt cttcctggaa gatgcggatc ttgcgctgtt tttcgaatgt gagccgcgac
     4201 gcggtgatgt tcgcgatcgc tccgttcctg aatttgaggc ggacgttcgc aatatcttca
     4261 aaaggggtca ggacattcac gccgatcgcg tcaaaatgct cgaaggggga gtccacgagc
     4321 cccagcacga tatcaagatc atggatcatg aggtccagca cgactccgca gtcgttgatc
     4381 cttcccgtga agggcccgag gcggtggatc tcgaggaacc ggatcttctt tgcgatctcc
     4441 cggacctgtt tgaaggcggg attgtggcgc tcgatatggc cgacctggag ggtacagcga
     4501 cgcttccggg aaatcctgat cagttcgtcc gcttcacgga gtcgcaacgt gatgggtttt
     4561 tcgaccagga cgtggacacc tttttccagg aattcccttg caacggtata gtgggtggag
     4621 gtcggggtgg cgatgctgac ggcgtcgacc ttcccgagga ggtc
//
LOCUS       DAOOBX010000027         4479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_21867_length_4479_cov_3.388110, whole genome shotgun sequence.
ACCESSION   DAOOBX010000027 DAOOBX010000000
VERSION     DAOOBX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21867_length_4479_cov_3.388110"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLY30_00810"
     CDS             <1..988
                     /locus_tag="PLY30_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64670.1"
                     /translation="DELDGVIHGDRRSPRNAVTRVVTAPVYLTRSFLFGLPNKDEEVN
                     KEGKKSVEAAAQDLQQQIELESLMIEAQNLEISRLEKEHEILTAKASLAGGYKFRGAF
                     VKVPYVFIDEAVQSAKRIVPKKQRKDVLLNRMDKESHKLEGTKKRLADVEAAIAAKSA
                     PSASVVPVAESVSADAKPAGTAPVAASAPDEKALREEITGLFERLEIQYSMYAEEQGL
                     LSEEYRNKLDVEMKASGADKEKVRRYKEFRELEASLAGVIKKEIEITSEESGILQKRL
                     AEIERLLPEVKSKAMTQDMLTEKTRTEQRLTDLDVRRDFLTKEMTRYERKSL"
     gene            999..1163
                     /locus_tag="PLY30_00815"
     CDS             999..1163
                     /locus_tag="PLY30_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64671.1"
                     /translation="MDLIKRLCFCLLLAVLVPSLSGCGYTTKTVLPRGIKTIFVETVK
                     NKIPISDLRS"
     gene            1173..1529
                     /gene="lptE"
                     /locus_tag="PLY30_00820"
     CDS             1173..1529
                     /gene="lptE"
                     /locus_tag="PLY30_00820"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HPW64672.1"
                     /translation="MEMDITNAVIARLQVDGNLQVVSEGRADAILQMDLTAYEQEGLR
                     FNQLESVEEYRMFIVMAIRLVDGKNREIIWEEPDFSGDAEYYVTDIKQIGEQAAVEQA
                     YGKLAKNIVDRIVEDW"
     gene            1592..2545
                     /gene="holA"
                     /locus_tag="PLY30_00825"
     CDS             1592..2545
                     /gene="holA"
                     /locus_tag="PLY30_00825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPW64673.1"
                     /translation="MKTNLFLIIGDPFLRSRRVKALISGLEKEAGTNLSQQTFRLSEI
                     SFETVLASARTLPFLAAGQVLLVREAEALKDSGAELLTNYVKTPAKDTILILEADASE
                     GLAEVIKTVKAAGQVITLAKEESRTVAQGYLQQKLARFKKSMTPGAKRKLLEMCGDAV
                     GFLDTMLDRVIQYAGEKTVIDEDMVSEFGENWREANVFQLTNAILARDRVQALRMFRE
                     LAGSGDSDPQSLVGIIHWQMRQLWHAAVLREAGIPDVDVLSRVKVPAFRKAPFLASVS
                     RYGLPRLEAAIEALYQLDKKAKTGLAKAVPGIESWLLEFVA"
     gene            complement(2554..2820)
                     /gene="rpsT"
                     /locus_tag="PLY30_00830"
     CDS             complement(2554..2820)
                     /gene="rpsT"
                     /locus_tag="PLY30_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPW64674.1"
                     /translation="MPNIASAAKRMRSDAKKRARNQAVLSELKTLSKKLSQLASNPEK
                     AKTTAAELVTRYDRAVSKGVIPRGRADRRKSRIAAFLSKLSKKQ"
     gene            2921..>4479
                     /gene="murJ"
                     /locus_tag="PLY30_00835"
     CDS             2921..>4479
                     /gene="murJ"
                     /locus_tag="PLY30_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPW64675.1"
                     /translation="MIKAAAVVSAMILLGRILGLIRDIVSAERFGTGWQWDAFIYAFM
                     IPNLFRRVVGEGGLTSAFIPVYNDIRTKQGIPEAFRFANITLCILATGLLIFVLAVQG
                     GVTLLLNAGTYNPTITLMLQLLQVLFPYLWFLSLYALGMGILNSHGRFFAGSLSSVIL
                     DGVWIAAVLWVPAWAGTDIVARLRWLAYAILFGGFLHIAAEYPSLHQIGFRIRWIWNT
                     AHEGLRRVWKLLIPVVLGFAVVQINITVDSTIGLLIGPGANSSLWYGNRLMQFPLGIF
                     ALAMGTALLPMMSRQIAEGRKEAAKRSLSFALRSIFFIIIPSSVGLIAFRNEIMRFLF
                     ERGEFDAVSTARSAAVLLGYTIGLFAYSGQKIMATGFYAAHDSKTPMKTAVISLVVNI
                     TFNLILMVPFREAGLALATSISGIIQFLQLVYYYPKKVGEFPAREVLLSFLRILVAGL
                     LMGVVCRVSLGVFRHFYPGTGTFAQGIQLFGSMGVAAVSYFVFCFFGRVPEMHEAVDW
                     MRRRKKKSALDV"
BASE COUNT          882 a         1226 c         1391 g          980 t
ORIGIN      
        1 cgatgagctc gatggcgtca tacatggcga ccgccgctcg ccccgtaatg ccgtgacccg
       61 ggtcgtgacc gcgcctgttt atctcacacg atcattcctg ttcgggttgc cgaacaagga
      121 tgaggaagtg aacaaggaag gcaagaagag tgtcgaggcc gcggcccagg accttcagca
      181 gcagatcgag ctcgagagtc tgatgatcga agctcagaac cttgagattt cccgccttga
      241 gaaggagcat gagatcctga ccgccaaggc gtcattggcc ggcggctaca agttccgcgg
      301 ggccttcgtc aaggtcccgt atgtttttat tgatgaggct gtgcagagcg cgaaacgcat
      361 cgtgccgaag aagcagcgca aggacgtgct gttgaaccgc atggataagg aaagtcacaa
      421 gcttgaaggc acgaaaaaac ggctcgctga cgtggaagcc gcgattgccg ccaagtccgc
      481 tccgtcggct tcggtcgtgc ccgtggccga atccgtttcc gcggacgcga agcccgccgg
      541 gacggcgccc gttgccgcgt cggccccgga tgaaaaggct ttgcgtgagg agatcaccgg
      601 actttttgaa cggctcgaaa ttcaatacag catgtatgcc gaggaacagg ggcttctgag
      661 cgaggagtac cgcaataaac tggatgtgga gatgaaggcg tccggggctg acaaggagaa
      721 ggtcaggcgg tacaaggaat tccgcgagct tgaggcctct ctggccggtg tcatcaaaaa
      781 ggagattgag atcacttccg aggaaagcgg tattctccaa aagcgcctgg ccgagatcga
      841 gaggctcttg cccgaagtaa aatcgaaggc catgacgcag gatatgctca cggaaaaaac
      901 gcgcacggag cagcgtctca cggacctcga cgtgcggcgt gattttttga cgaaagaaat
      961 gacgcgttac gaaagaaagt ccttatagga gttgccccat ggatctgatc aagcgtttgt
     1021 gtttctgtct tcttttggcc gtcctcgttc cgtcgctgtc gggttgcggt tacacgacca
     1081 agaccgttct ccctcggggc atcaagacga tttttgtgga aacggtcaag aacaagattc
     1141 cgatctcgga cctgcgttcc taacagcagg gcatggaaat ggacattacg aacgccgtga
     1201 tcgcgcgtct gcaggtagac ggcaatcttc aggtggtctc ggaaggccgg gcggatgcga
     1261 ttctgcagat ggacctcacc gcgtatgaac aggaagggct ccgttttaat cagcttgagt
     1321 cggtcgagga ataccgcatg ttcattgtta tggccatacg cctcgtggat ggcaagaacc
     1381 gcgagatcat ctgggaagag cctgattttt cgggcgatgc ggaatactat gtgacggaca
     1441 tcaaacagat cggcgagcag gccgctgtcg agcaagccta tggcaagctt gcgaagaata
     1501 tcgtcgaccg gatcgtcgaa gactggtgac cgggccccgt tcccgatccc gggggcgtac
     1561 ggtttccgac attgacccca cgcgagacgg gatgaaaacg aacctctttc tgatcatcgg
     1621 tgaccccttt cttcgctccc ggagggtcaa ggccctcatt tccgggctcg aaaaagaagc
     1681 cggtacgaac ctgtcgcagc agacattccg ccttagtgaa atctcctttg agaccgtact
     1741 cgcaagcgcc cgaacccttc cgttccttgc ggcagggcag gtccttttgg tccgcgaagc
     1801 ggaagcactg aaggattccg gcgcggagct cctcacgaat tacgtcaaga cccccgcgaa
     1861 agatacgatc ctcatcctgg aagcggacgc ttcggaaggc ctggccgagg tcattaaaac
     1921 ggtcaaggcc gcggggcagg tgatcacgct cgcgaaagag gaatcccgca cggtcgccca
     1981 gggctatctt caacagaagc ttgcccggtt caagaagtcc atgacgcccg gggcgaagcg
     2041 gaaattgctg gagatgtgcg gtgatgccgt ggggttcctt gacacgatgc tcgaccgcgt
     2101 gatccagtac gccggtgaaa agaccgtgat cgatgaggac atggtctcag agtttgggga
     2161 gaactggaga gaggcgaacg tcttccagct gacgaacgcg atcctcgcgc gggaccgcgt
     2221 ccaggcgctc aggatgttcc gcgagctcgc ggggagcgga gattcggacc cgcagtcact
     2281 ggtcgggatc atccattggc agatgcggca attgtggcat gcggccgtgc tccgggaagc
     2341 ggggatcccc gatgtcgatg tgctctcgcg cgtgaaggtc ccggcgttca ggaaagcgcc
     2401 ttttcttgcg tcggtgtcac gatacggtct gccgcggctt gaggcggcaa tcgaggcgtt
     2461 gtatcaactc gacaaaaaag ccaagaccgg cctggcgaag gcggtgccgg gcatcgagtc
     2521 ctggcttctg gaatttgtgg cgtgacgaac agcttattgc tttttcgaaa gcttgctgag
     2581 gaaagccgcg atccgggatt tcctgcggtc agcgcgaccg cgcgggatga cgcctttgga
     2641 aacggcgcgg tcatagcggg tgacaagctc cgcggccgtc gttttcgcct tttcagggtt
     2701 cgaggcgagc tgggagagct ttttggaaag cgtcttgagc tcggaaagga ccgcctggtt
     2761 cctggcgcgt ttcttggcat cactgcgcat ccgttttgct gctgaagcga tgttcggcat
     2821 tgaaaggact cctttgggtg aaaaaagtgg cgatagggta gcacagggta agccgtctgt
     2881 caatggacat ccccgcggaa acccttcggg ggaacgccat ttgatcaagg ctgccgccgt
     2941 tgtcagcgcg atgatcctgc ttggccgcat cctcgggctc atccgcgaca tcgtcagcgc
     3001 ggaacggttt ggcaccggct ggcaatggga cgccttcatc tacgcgttca tgatcccgaa
     3061 cctcttccgc cgggtcgtgg gagaaggagg cctcacaagt gccttcattc ccgtgtataa
     3121 cgacatcagg acgaaacagg ggatcccgga agccttccgg ttcgcgaata tcacgctttg
     3181 tatccttgcg accggtctcc tgatcttcgt ccttgccgtt caggggggcg tgaccctgct
     3241 tttgaacgcc ggaacgtaca atcccaccat cacgttgatg ctccagcttc ttcaggtgct
     3301 cttcccgtat ctgtggttcc tttcgcttta cgccctcggt atgggaatcc tgaacagtca
     3361 tgggcgtttc ttcgcgggct ctctcagctc cgtcattctc gacggggtgt ggatcgccgc
     3421 ggtgctttgg gttccggcct gggcgggcac ggacattgtg gcgcgcctgc ggtggctcgc
     3481 gtacgcgatc ctgttcggcg gattcctgca catcgcggcg gaatacccgt cgctccatca
     3541 gatcgggttc cggatccgct ggatatggaa taccgcccat gaggggctcc gccgggtctg
     3601 gaagctgctg atccccgttg tgctcggttt tgcggtcgtc cagatcaata tcacggtcga
     3661 ctcgacgatc ggtcttttga tcggaccggg cgcgaactcg agcctctggt acggaaaccg
     3721 tctcatgcag ttcccgctcg gcattttcgc gctcgcgatg gggaccgcac tgctcccgat
     3781 gatgagccgt cagatcgccg agggccggaa agaggccgcg aagcggtcgc tctcctttgc
     3841 ccttcgcagt atttttttca tcatcatccc gtcctcggtc ggattgatcg cgttccggaa
     3901 cgagattatg agattccttt ttgagcgcgg agagttcgac gctgtatcga cggcgcgaag
     3961 cgccgcggtc ctgctcggtt acacgatcgg tctcttcgcg tattccggcc agaagatcat
     4021 ggcgacgggg ttttacgcgg cccatgattc gaagaccccg atgaaaacgg ccgtgatctc
     4081 gcttgtcgtc aatattacgt tcaacctcat cctgatggtg ccgttcaggg aggcgggcct
     4141 tgcgctcgcg acctcgatct cggggatcat ccagttcctg cagcttgtct attattatcc
     4201 gaaaaaggtg ggagagtttc ccgcccggga ggtcctcctg tctttcctgc gtatcctggt
     4261 cgcgggcctt ctgatggggg tcgtctgccg cgtgagtctc ggggtttttc gtcattttta
     4321 tcccgggacc ggcactttcg cgcagggaat ccagctgttc ggaagtatgg gggtcgcggc
     4381 cgtttcctat tttgtgttct gcttctttgg gcgtgtcccg gagatgcatg aagcggtgga
     4441 ttggatgcgc cgacggaaga aaaagtccgc gctggatgt
//
LOCUS       DAOOBX010000028         4425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_22169_length_4425_cov_3.261098, whole genome shotgun sequence.
ACCESSION   DAOOBX010000028 DAOOBX010000000
VERSION     DAOOBX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22169_length_4425_cov_3.261098"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4425)
                     /locus_tag="PLY30_00840"
     CDS             complement(<1..>4425)
                     /locus_tag="PLY30_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64676.1"
                     /translation="LPSITNVANHSGRVLGEELRPFEIRDNYDWVELEKAGVVKVRET
                     IPELRLAERQEELARIARIAADLKARGIKTTVPRIASNSKRVTGRALSEVQIRKYDWK
                     ELKSAGVKPPEDMAKRAGTLRRAVAAILQESPLGPITYGVIARKMAEDSEYPVTINWK
                     ALTKYVSGRPEFSRIARIVKASPRAVPQPARPAEERSDTAIPESAVAPVNFYLEVRGF
                     GKIRSYLRHFDPANPGVVSGLRKVSPGRYKGEYLIEWDKKGAGATLLDLLTQFRKVQP
                     ISPRDEFLIAGMVYRDPLGKRSIRAGESYILQPERNRIVFADDGESGELEGDLDLDVL
                     TLRMIAAGARAVREKTEDASREFYNAASRLLTGSALAVGEVDDAWFARFETIYQRYAG
                     PLGDSKGNRQVLAKLKDLRGVVSEKAVRRPEARAGGEAVKEMPGKGPRAELRSSRKRF
                     EASEKGKNRRGVGETELYEILKAGLSAVPPAIVDELRKIRDVGAWRNILRTAGLDVVD
                     LIRGKFLFPIVERFLQSQEQSAAQRVSNRLRFGGQGRYLIAYLGDDFDFIVKMPVTPE
                     KYDVVRRIWLGHGFKVARQRLNGFAVPTLVLDAAGGNKKEFVYHADGTERRKEELAIV
                     QFKVERLMDKLKSLARGGDLEGAKTLIGKFRECLIGIFRRGVIDADFDAVLYNFGVDG
                     KSGNILIFDFGDLTTGYDMENMFYERLWGMNKSIEADLRELNGELGDYFRDRGRFVLE
                     DFFDENGESLYGVDYKPGGSGVEGESSGKASDLEMTFPYPEEDIRAMFASHTVHEASR
                     SEMRDGREEAAATPDEPLYERKLSSEEYTAYFEEVMDALTAVNFGVAAAVQVMTSYLR
                     RQRKVESQRRRRFGEIGRFQKDHSRIFAPLQLLLGHLGDPPVGRSLWFIARFLLSLKD
                     AGLFSWSLSVLLATSRDPRRDPAVAGNAEALLRMVRELGLNPGVPVSWAARAVLVAWE
                     RQPLINPLDLYMKKFDSEKKFRVADEMVYVRGKRGRNERDMDTARARKQSIGRMREQE
                     LDEEMKRALGRSATRAELREAEGYENVHGPVHLTEEIAIAIRTADDLADYAGKLIAQI
                     NRNNPKQEPLTLHSIWQDPAKYENVFYRPFYELFFMKLQMDFEGAVPFVEAARDVMRT
                     RGITGLRKIMDSDGGSLANLFSKIRHDGKSAASAIDFYGQLCNFLTGNLDGYFGHIYD
                     ELMRTRPPIAIARPGLSYHSRADSTIRLRDRALLFGSDRAIAKYSQIRGSVIEMTRTD
                     MQTRKNSAHAFKIQQAIDSWEKRAEELNSEMLKERIARFKSSGLVIEYDSDLPLIIAD
                     AEQNVARVVHTERLTHALNMSSAFIENAAPEEIAAYINTAQAWLDIYTEQRGAEASGA
                     DGQDVAASDPVIVRVEAERLRFNLEFFDREDSGLVPVEARRDEWPAGTQGPTLEGLLA
                     ARMKEHVLKRYKGFLESVIKQEAEAAKILKTAREIKRE"
BASE COUNT          885 a         1434 c         1106 g         1000 t
ORIGIN      
        1 tcctcccgct tgatctcccg cgctgtcttg agaattttcg cggcttccgc ttcctgcttg
       61 atgacagatt ccagaaatcc cttgtaacgt ttcagcacat gttctttcat gcgcgcggca
      121 agcaatccct cgagcgtggg gccttgcgtc ccggccggcc attcgtcccg tcgggcctcc
      181 acggggacca ggccgctgtc ttcccgatca aaaaattcga gattgaaccg aaggcgctcc
      241 gcctccaccc ggactatgac cgggtcgctg gccgcgacgt cctgaccatc ggctccggaa
      301 gcctccgctc ccctttgttc cgtgtaaata tccagccatg cctgagcggt attgatatag
      361 gccgcgatct cctccggcgc agcgttctcg atgaacgccg aagacatgtt gagggcgtgc
      421 gtcagacgtt ccgtatggac gacacgcgca acgttctgct ccgcatcggc aatgataagc
      481 ggaaggtccg aatcatactc gatcacgagg ccggaactct tgaacctggc gatcctctcc
      541 ttcagcattt cggaattgag ctcctcagcc cgcttttccc aggaatcgat cgcctgctga
      601 atcttgaacg cgtgcgcgct gttcttgcgt gtttgcatat ccgtcctggt catttcgatc
      661 acactgccgc gtatttgcga gtattttgcg atggcacggt cactgccgaa cagaagcgcc
      721 cggtccctga ggcgaatcgt gctatcggcg cggctgtggt atgagagacc cggtcgagcg
      781 attgcgatcg gagggcgtgt tcgcatcagc tcgtcataga tatgaccgaa ataaccgtca
      841 agattcccgg tcagaaaatt gcacagttgc ccgtaaaaat cgatggcgct ggccgcgctc
      901 ttgccatcat gccggatctt gctgaacaga ttcgcaagag aaccgccgtc tgaatccatg
      961 attttccgca atcccgtaat cccccgggtt cgcatcacat cccgggcggc ctcaacaaat
     1021 ggcacggccc cttcaaaatc catctggagt ttcatgaaga aaagctcata gaacggcctg
     1081 taaaaaacat tttcatattt tgccgggtcc tgccagatcg aatggagggt taagggttcc
     1141 tgcttgggat tgttgcggtt gatctgcgcg atcaatttcc ccgcgtaatc cgcaaggtcg
     1201 tccgccgtac ggatcgcaat cgcgatctct tccgtcagat ggaccgggcc atggacgttt
     1261 tcatatccct ccgcctcgcg aagttccgcg cgcgtggcgc ttcgaccaag cgctctcttc
     1321 atctcctcat caagttcttg ttccctcatc cgtccaatgg attgcttcct cgcccgagcg
     1381 gtatccatat cacgctcatt ccggccgcgt ttaccgcgaa cgtagaccat ctcatccgcc
     1441 accctgaatt tcttttcgct gtcgaatttt ttcatgtaaa ggtccagggg gtttatgagc
     1501 ggctgccttt cccacgcgac caggacagcc cgggcggccc acgataccgg cacaccggga
     1561 ttcagtccga gctcgcggac catccggagc agggcttccg cgttcccggc cacggccgga
     1621 tcacgtctcg ggtcccggct tgtcgccaga agcaccgaca ggctccagct gaaaagaccc
     1681 gcgtccttca gggaaagcag gaaccgggcg atgaaccaga gtgatctacc gaccggcgga
     1741 tcgccaagat gtcccaaaag aagctggagc ggcgcgaata tccgggaatg atctttctgg
     1801 aatcttccga tctcgccgaa ccgcctccgc ctttgagatt ccacttttct ctgcctgcga
     1861 aggtaggacg tcatcacctg aacagccgcg gcaaccccga aattaacggc ggtgagagcg
     1921 tccatgactt cttcaaaata cgccgtatat tcctcgctgc tcagcttacg ttcataaagc
     1981 ggctcatcgg gagttgccgc ggcctcttcg cgcccgtcgc gcatctcaga ccgggaggct
     2041 tcatgcacag tgtgactcgc aaacatggcg cgaatatcct cttcgggata cgggaaggtc
     2101 atctcaagat ctgaagcttt tccggaagat tccccctcca ccccggaacc cccgggcttg
     2161 taatcgaccc cataaaggct ttcgccgttt tcatcgaaaa agtcctccag gacgaacctt
     2221 ccccgatcgc ggaaataatc ccccagctcc ccgttaagtt cccgaagatc ggcctcaata
     2281 ctcttgttca tgccccacag gcgttcgtag aacatgtttt ccatatcgta gcctgtcgtc
     2341 aggtccccga aatcgaagat gagaatgttc ccgcttttgc catccacacc aaagttatag
     2401 agcaccgcgt caaagtccgc atcgatcacg ccacggcgga agataccaat gagacattcc
     2461 ctgaattttc caatcagggt cttcgccccc tcaagatcgc ccccgcgggc cagggatttc
     2521 agcttatcca ttaatcgctc gaccttgaac tgaacgatcg ccaattcttc tttcctgcgc
     2581 tccgttccgt ccgcgtggta gacaaactct tttttatttc cgcccgccgc gtccagcaca
     2641 agggtcggca ccgcaaaacc gttgagtctc tgccgggcca ctttaaatcc atgcccaagc
     2701 caaatgcgcc gcaccacatc gtatttttcc ggggtcacgg gcatcttcac gatgaaatca
     2761 aaatcatcgc ccagataagc tatcagatat ctcccttggc cgccaaagcg caggcggttg
     2821 ctgacccgtt gcgccgcgga ttgttcctgg ctttgcagaa atctctccac gatggggaaa
     2881 aggaacttcc cgcggatcag gtccacaacg tcgaggccgg cggtccgcag aatatttctc
     2941 caggcgccaa catcccggat cttccgaagt tcatccacaa tcgccggcgg cactgcggac
     3001 aatccggctt tcagtatctc atagagttcc gtctcaccca ctccgcgccg gtttttgcct
     3061 ttttcagaag cctcgaagcg cttccgcgag ctccgtagtt ccgcgcgcgg ccccttcccg
     3121 ggcatttctt taacagcctc tccgccggcg cgggcttccg ggcggcggac ggccttttcc
     3181 gataccacgc cgcgaaggtc cttcaacttc gcgaggacct gcctgttccc tttggaatct
     3241 ccgagcggtc ccgcgtaccg ctgatagatc gtctcgaacc tggcgaacca cgcgtcatca
     3301 acctcaccca cggccaacgc ggaacccgtc aggagcctgg aagccgcgtt ataaaattca
     3361 cgggatgcgt cctccgtctt ctcccggacc gcgcgggccc ccgcggcaat catccggagc
     3421 gtgagtacat cgagatccag atccccctcg agttctccgg attccccgtc atcggcaaaa
     3481 acgatcctgt tacgctccgg ctgcaggata taggactcac ccgcgcggat cgacctcttc
     3541 ccgagagggt cccggtagac cattcccgcg atcaggaatt catcccgcgg ggagatcggc
     3601 tgaaccttcc tgaactgcgt cagaagatca agaagggtcg cgccagcccc ctttttgtcc
     3661 cattcaatca gatactctcc cttgtaacgc cccggcgaca ccttgcggag gccgctcacc
     3721 actccgggat tcgccggatc aaaatgcctg agatagctgc ggatcttccc gaatccccgg
     3781 acctcgaggt aaaaattcac cggcgcaacg gcagattccg gaatggccgt atcggatctt
     3841 tcttccgccg gcctggcggg ctgaggcacg gcacgcggcg atgctttcac aatcctggca
     3901 atccgggaaa attccggacg ccctgaaaca tatttcgtga gcgccttcca gttgatcgtc
     3961 actggatact cggagtcctc ggccattttt ctcgcaatca ccccataagt aatcggccca
     4021 agaggactct cctggaggat cgccgccaca gcccggcgaa gggtcccggc tctttttgcc
     4081 atatcctccg gcggttttac tccggcagat ttgagttctt tccagtcgta cttgcggatc
     4141 tgcacctcgg ataatgcccg tccggttacc cttttagaat ttgacgcgat cctcggaacg
     4201 gtcgttttga taccccttgc cttcagatcg gccgctatcc tcgcgatcct ggcaagctcc
     4261 tcctgccgtt ccgccaggcg aagctccggg atcgtttcgc ggaccttgac cacccctgcc
     4321 ttttcaagct ccacccaatc ataattgtca cgaatttcaa acggcctcag ttcttcgcca
     4381 aggaccctcc cggaatgatt cgcgacattg gtgatgctgg ggaga
//
LOCUS       DAOOBX010000029         4392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_22390_length_4392_cov_4.683422, whole genome shotgun sequence.
ACCESSION   DAOOBX010000029 DAOOBX010000000
VERSION     DAOOBX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22390_length_4392_cov_4.683422"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4392)
                     /locus_tag="PLY30_00845"
     CDS             complement(<1..>4392)
                     /locus_tag="PLY30_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64677.1"
                     /translation="SYDARVSYSVSVDEKVPVGEGETAGYATVAKFTLVERVSRAHML
                     KLLDQIPSAFDEIEKESDERRNARAAIADGESFTMSRSGGDDITCSWASVSDPTVSYT
                     ITEDVHSLLGKANASFKKSTRISREEVATLPEGLAAVEEAPAGLTDSRFGAAPIRELL
                     LGGLASDPNWDRAVVRLVEGAAEDGTLKWTGYVIVSEDGKSMRSAILSLELDPVTGDY
                     RDLDPSTPDLIDRVQYIETISVGADDAVTPKGKTAAEVFGDRVAAGDWPVSRVRISTG
                     SFAAAAWTPESDFYDLAGYDSEQLRIAFFEGAGDPEQPQFMTRVEKAEVLDVVPLMRD
                     EGMRRAALAALNDPNAVFYRIEGSGKDKSYQWTSSFDSNVSYTITVDIKVAVGDAENF
                     TQEATFTTQTRVSRAVIATAYLDQLARVLVPLVDGASGVTIMGLLNAASLADFKALVA
                     GSSLPDELKDAIAGPLDTATRVRSGGLSEKDWQAVVSVMRDPLVITNALISAVYPLDE
                     AAALTGALRNAPDLAAFKARVASAGLSADIKAYVATLNAADWPVVNAEIDRPKGAASI
                     TPREIEFFRLALAESTELVTSFTKTETIDTRGGNQVSYNWSSADGIKNYSLACSSVAI
                     TEMDGTITYEAETAFSVSQRYARIDADYIKQLVPGGESFTSLPGDRGDADSQYARFLA
                     ALEILQDPDNMILITMTMNKEGFSYSWELPDRSRTFTLNQERARVLVSQDPDVYGDNW
                     TFTFSVAERVSQTAVSGKEDYLTPEKKTQYLKALADMDHGTDLMMTQTGKDVSYSWAS
                     PDRARTYTLTKTFAKVSGAAEAVGKWDFNIQTRVRRSEVELYAENLPAGPSREQYLRA
                     IRDTASWTKYTKTESGTDTSYKWTGRNGAVTYSLSGTRSLMEHIEDGVLSLRNTYSYS
                     FSAMDRVAKEFVNGFSDKLPDPVQKEHYLSALGFTDESTIYNRTISGDDVSYRWTDPD
                     ELTTYVVGASKTYELIPESVDANGKVVYAMEGGNYKYNVSEKWSFRSEVIVLLEEVTG
                     AGFANEAFLETKLLRKQFHDAVTGKAAGLATWYTKTVTGDTVTYSWEDEATGRMHTLS
                     REPAVVTGVQGPVFQWSFKTDTMADATVLNAYAGFLDDDMRSDFDVAILDTAKDIFTK
                     STIGAEESFSWQSRDRGRSYVMSASRTVDPATGAPAYAKWGFSTQMTLSRAALARYVP
                     SFEDPEMAAALSEALRLDDFDPLGSVTLSLTGRDISISWRSQDLAKSCTLGLTTAKTL
                     KTETRDADGNVTDRTYEERPKWSFAVSTKVLKAAVSGSGAEFETDAAERQYLDALDIL
                     ADDLTDSVDNATVFTKVVSGRSVSYSWQDLDGYTTHRLSPSRQKIYDPETETVNGVER
                     VRCDAAGKVIYKSTYKEVEKWDISSETLVLRDEIARHADADGAMVGRELLLETRELRE
                     HYLKVLAETPASTAFTKSVSGGDVSYSW"
BASE COUNT          809 a         1428 c         1249 g          906 t
ORIGIN      
        1 ccaggaataa ctgacatcac cgccgctcac gctcttcgta aaggccgtcg acgccggcgt
       61 ttcggccaga actttcaggt agtgctcgcg gagctcccgg gtttccagca ataactccct
      121 gcccaccatc gcgccgtccg cgtccgcgtg cctggcgatc tcatcgcgaa gcaccagcgt
      181 ctcggaactg atgtcccact tttcaacttc cttgtacgtg gatttgtaaa tgaccttgcc
      241 ggcggcatca caacggacgc gctccacccc gttgacggtt tcggtttcag ggtcatagat
      301 cttctgccgc gacggggaaa gacggtgcgt cgtgtacccg tccaggtcct gccagctgta
      361 ggagaccgag cggccgctca ccactttcgt aaacaccgtg gcgttgtcga cgctgtcggt
      421 aaggtcatcc gccaggatgt cgagggcatc caggtactgc cgttccgcgg cgtccgtctc
      481 gaattcggcg ccgctccccg acaccgcggc cttcaaaact ttcgtgctga ccgcaaaact
      541 ccacttcgga cgttcctcat aagtccggtc cgtcacgtta ccgtccgcat cccttgtttc
      601 ggtcttcagc gtcttcgcgg tcgtgagccc aagcgtgcag gacttcgcga ggtcctgact
      661 ccgccagctg atgctgatat cccggccggt cagggacagc gtcaccgatc cgagcggatc
      721 aaaatcgtcc agccggagcg cctcgctgag agcggcggcc atttcggggt cctcaaaaga
      781 agggacgtac cgcgcgagcg cggcccgcga aagcgtcatc tgcgtgctga atccccactt
      841 cgcgtaggcg ggcgcgccgg tcgcgggatc gaccgtcctg gacgcgctca tgacgtagct
      901 gcgcccccga tccctgctct gccaggaaaa actctcttcg gcgccgatcg tggacttggt
      961 gaaaatatct tttgcggtat caagaatggc aacgtcaaaa tcactgcgca tatcatcatc
     1021 cagaaaaccg gcgtacgcgt tcaggaccgt cgcgtcggcc atcgtatcgg tcttgaaact
     1081 ccactgaaag accggcccct gaaccccggt caccaccgcc ggctcgcggc tgagggtgtg
     1141 cattctcccg gtcgcctcat cttcccacga gtaggtcacg gtatcacccg taaccgtttt
     1201 ggtataccag gtcgcgagtc ccgccgcctt accggtcacg gcatcatgaa actgcttgcg
     1261 aagcagtttt gtctcgagaa aagcctcgtt agcgaatccc gcaccggtca cctcctccag
     1321 caggacgatg acctcggaac ggaaagacca tttttcggaa acgttgtact tgtagttccc
     1381 gccttccatc gcgtagacca ccttcccgtt cgcgtcaacg gactcgggga tcagctcata
     1441 ggtcttgctc gctccgacca cgtaagtcgt caactcgtcc ggatcggtcc agcggtagct
     1501 cacatcgtcc cccgagatcg tccggttgta gatcgtcgat tcgtccgtaa atccgagggc
     1561 ggacagataa tgctctttct ggaccggatc aggaagcttg tccgaaaaac cgttcacaaa
     1621 ctccttcgcg acgcggtcca tcgcggagaa cgagtaactg taagtgttcc gcagggacag
     1681 aacaccgtcc tcgatgtgtt ccatgaggct cctggtcccg ctcaggctgt acgtgacggc
     1741 accgttgcgg cccgtccact tgtagctcgt gtccgtcccg ctctcggtct tcgtgtactt
     1801 cgtccagctc gccgtgtcgc ggatggcgcg aaggtattgt tccctcgatg gaccggcggg
     1861 caggttctcc gcgtagagct cgacctcgct tctccggacg cgcgtctgga tattgaaatc
     1921 ccatttaccg accgcctcgg cggcgcccga aaccttcgcg aaagtcttgg tcagggtata
     1981 cgtcctcgcg cggtcagggc ttgcccagga gtagctcaca tccttcccgg tctgcgtcat
     2041 catcagatcg gtgccgtggt ccatatccgc gagcgctttc aggtactgcg ttttcttttc
     2101 gggcgtcaga taatcttctt ttcccgacac ggccgtctgg gaaacgcgtt ccgcaacgga
     2161 gaacgtgaat gtccagttat ccccgtaaac atcgggatct tgcgatacga ggaccctggc
     2221 gcgctcctgg ttcagcgtga aggtcctgga tcggtcggga agttcccacg aataactgaa
     2281 cccctccttg ttcatcgtca tcgtaatcag gatcatgttg tcgggatcct gcaggatctc
     2341 aagcgccgcg agaaaccggg cgtactggga gtccgcatca ccccggtctc cgggaaggct
     2401 cgtgaacgac tcgccgccgg ggacaagctg cttgatgtaa tccgcatcga tcctcgcgta
     2461 acgctgggaa actgaaaaag ccgtctcggc ttcataggtg atggtcccgt ccatttccgt
     2521 gatcgccacg gaactgcagg cgagagaata attcttgatg ccgtccgcgc tcgaccagtt
     2581 gtagctgacc tgattcccgc cccgggtgtc gatcgtctcg gttttcgtga acgaggtcac
     2641 aagttcggtg ctctccgcca gcgcgagcct gaaaaattcg atctcacggg gcgtgatcga
     2701 ggccgcgccc ttcggacggt cgatctccgc gttaacgacc ggccagtccg cggcgttcag
     2761 cgtggcgacg taagccttga tgtcggcgga aaggccggcg gacgcgactc gcgccttgaa
     2821 cgccgcaagg tcgggggcat ttctgagcgc cccggtcaag gccgccgcct catcaagagg
     2881 atacaccgcc gagatcagcg cgttcgtgat aacgagagga tcgcgcatga cggaaacgac
     2941 cgcctgccag tccttttcgg ataaaccgcc gcttcgcacc cgggtggcag tgtcaagcgg
     3001 cccggcgatc gcgtctttca gttcatcagg cagggatgaa ccggccacaa gcgccttgaa
     3061 atcggcgagc gatgcggcgt taagaagacc catgatcgtc acacccgagg ccccatcgac
     3121 cagcggcacc agcacgcgcg cgagctggtc aagataggcc gtcgcgatca ccgcgcggct
     3181 gacccgcgtc tgcgtcgtga acgtcgcttc ctgcgtgaag ttctccgcgt cgccgaccgc
     3241 caccttgatg tcgaccgtga tggtgtacga aacattcgaa tcgaacgacg atgtccactg
     3301 ataactcttg tccttgccgc tgccctcgat gcggtagaag accgcgttcg gatcgttcag
     3361 ggcggcgagc gcggcgcggc gcattccctc gtcgcgcatc aggggaacaa catccagcac
     3421 ctcggctttt tcaacccggg tcatgaactg cggctgttcc gggtccccgg cgccttcaaa
     3481 aaaagcgatc cggagctgtt cggaatcgta gccggccaga tcataaaaat cgctttcggg
     3541 cgtccacgcg gcggccgcga atgatccggt cgagatcctg acacgcgaaa caggccagtc
     3601 ccccgccgcc acgcgatctc cgaacacttc cgcggccgtc ttgcctttgg gagtcacggc
     3661 gtcgtccgca ccgactgaaa ttgtctcgat gtactggacc cgatcgatca ggtccggcgt
     3721 ggaaggatcg aggtcccgat agtctcccgt cacggggtcc aattcaaggc tgaggatcgc
     3781 tgagcgcatg gacttcccgt cttcggaaac gatcacgtag ccggtccact tcagcgttcc
     3841 gtcctccgcc gcgccctcaa caagccggac caccgcgcgg tcccagttcg ggtcactggc
     3901 aagccctccc agcagcagct cgcggatcgg cgcggcgccg aagcggctgt ccgtaagccc
     3961 ggccggagct tcctccacgg cggccagacc ttcgggaagg gtcgccactt cctcccggct
     4021 gatccgggtg ctctttttaa aactcgcgtt cgcctttcca agcagagaat gaacgtcctc
     4081 cgtgatcgtg tagctcaccg tcggatccga aacgctcgcc caggaacagg ttatgtcgtc
     4141 ccccccgctc cgggacatgg tgaagctctc tccgtcggcg atcgcggcgc gggcgttcct
     4201 ccgttcatcg ctttcctttt cgatctcgtc aaaggcactg ggaatctggt cgagaagctt
     4261 gagcatgtga gcgcggctca cgcgctcgac aagcgtgaat ttcgcgacgg tcgcgtaccc
     4321 cgccgtttca ccttccccga cggggacttt ctcatccacg gaaacactgt agctgaccct
     4381 ggcgtcatac ga
//
LOCUS       DAOOBX010000030         4291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_23073_length_4291_cov_3.430595, whole genome shotgun sequence.
ACCESSION   DAOOBX010000030 DAOOBX010000000
VERSION     DAOOBX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23073_length_4291_cov_3.430595"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..305)
                     /gene="rpsO"
                     /locus_tag="PLY30_00850"
     CDS             complement(36..305)
                     /gene="rpsO"
                     /locus_tag="PLY30_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPW64678.1"
                     /translation="MALAKTKKKEIISRNKKTKNDTGSSEVQIALLTERINGLSPHFG
                     KHVKDHHSRYGLIKMVSRRKRLLAYLKREDPEKYQKLIASLDLRK"
     gene            complement(351..1358)
                     /gene="ribF"
                     /locus_tag="PLY30_00855"
     CDS             complement(351..1358)
                     /gene="ribF"
                     /locus_tag="PLY30_00855"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HPW64679.1"
                     /translation="MRTVTDLHKFKAGRGRRLVLALGNFDGFHLGHQKLIRYVTTQAR
                     RHDALAAALTFRNHPQIVLHPEKEQRLLFSVGQKLFYLKQAGIDLCFAPAFTKAFSKM
                     PPEVFVEKVLVRKLRVFEVCMGYDARFGYRRCGTTDLMRDLAGKHGFLFRKMEPVMIG
                     GRPVSSSGLRELVSEGKVEKVSHCLGRPFSLFGKVVRGKGRGARLGAPTANVEVHANI
                     QLPVGVYVASARVIPETRWFPGVLNYGNRPTYPAFETPNPVLEMHMFDFKRKVYGKEM
                     EIAFHHFIRPERQFGSEEALKVRIRRDIETAKRWHLGHVLGKKPFTNCGGQGILPQPQ
                     R"
     gene            complement(1333..2070)
                     /gene="truB"
                     /locus_tag="PLY30_00860"
     CDS             complement(1333..2070)
                     /gene="truB"
                     /locus_tag="PLY30_00860"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPW64680.1"
                     /translation="MPIRPEFVDGVLLADKPETWTSHDVCAFIRKRFRFRKVGHAGTL
                     DPLATGLLVILIGKATKYSMALSACDKEYYGTMELGVQTDSHDRQGKVIAEAPWEHVT
                     IDLIREKARDFTGEIVQVPPMVSALKHEGTRLYKLARQGKTVPREGRRVTVHEFRFDK
                     MEGRFADFSAHVPKGTYLRTLVNDLGEVLGCHACLARLRRLCSGNFDITKSVTIDTLR
                     TMSLEELLPKMLPLGAVPQYANSHRPS"
     gene            complement(2074..2430)
                     /gene="rbfA"
                     /locus_tag="PLY30_00865"
     CDS             complement(2074..2430)
                     /gene="rbfA"
                     /locus_tag="PLY30_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPW64681.1"
                     /translation="MQGKRTERVGSLLQMELGKLILERVKDPRLGFVTLTHVKVTADL
                     KSAVVFFTVMGDAAKRKDSLDVLRRAAGFLQREVGKTLTMRYTPHLQFELDDMVDKSF
                     EIEALIRKIENPPTGQ"
     gene            complement(2453..>4291)
                     /gene="infB"
                     /locus_tag="PLY30_00870"
     CDS             complement(2453..>4291)
                     /gene="infB"
                     /locus_tag="PLY30_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPW64682.1"
                     /translation="KPKAQAPESPKVVPAPAPKPEPVKVEPPRIEVRFPITVGDLAQR
                     MNKRVPEVIKTLMGLGIFANVNQLLNEEIVFALADAVGVEIRKMEDEVVEKVQKAEAA
                     EDKSRLKLRPPVVTMMGHVDHGKTSLLDAIRSTNVAEREKGFITQHIGAYGVDIPGKG
                     HVTFLDTPGHEAFTAMRARGANVTDIVVLVVAGDDGVMPQTIEAIHHAREAGCPIVVA
                     INKCDLPVANPQQVMGGLQKQDLMPEEWGGKTICMKVSAKTGKGVPELLDMLLLQAEV
                     MELKANPDCPAVGTVVESHLSKGSGPIATVIVKKGTLRTGDIVVAGSYTGRVRVMKND
                     RAKTVHEAGPAYAVELSGLNGVPDAGEMLYAVEDEKTAKRITEQRILEIRERQMRGVS
                     KHMSLESLYSKISEGSFKELKLIVKADAQGSVEALTQSLEKLTTEKCRLHVIHGAVGG
                     INESDVMLAAASDAIVIGFHVKADPKALGIAEKEGVDVRFYGIIYDAVEKIRKAMEGI
                     LEPELEEVIEGKAQIRQIFTSSKVGTIGGARVIKGRLVRHHGVRVVRNNVVVFDGRVS
                     ALKHFKDDVKEVAEGHECGISLVGFNDLREGDIIESYRVDKIAATL"
BASE COUNT          829 a         1300 c         1197 g          965 t
ORIGIN      
        1 catggattct cagacaaccg gaaaagcagg agaaattact tcctgaggtc cagactggcg
       61 atcagcttct ggtatttttc cggatcttca cgcttcagat aggcgagcag ccttttgcgg
      121 cggctgacca tcttgataag accgtagcgg gaatgatggt ccttgacgtg ttttccaaaa
      181 tgaggggaaa gcccgttgat gcgctcggtg agaagagcga tctggacttc ggaggacccg
      241 gtgtcgttct tcgtcttctt gttcctggaa atgatctctt tcttcttcgt ctttgctaat
      301 gccatgcgtc tctgtaactc cttctctctc tgtcagttat gtcgtttttc tcatctctga
      361 ggttgcggga gtataccctg gcccccacag tttgtaaagg gcttcttgcc caaaacatgt
      421 cccagatgcc atcgttttgc cgtctcgata tcccttcgaa tccgcacttt cagcgcctcc
      481 tcggatccga actgcctctc cggcctgata aaatgatgaa aggcgatttc catttctttg
      541 ccgtagacct tccttttgaa atcaaacata tgcatctcaa ggacagggtt cggcgtttca
      601 aaggcaggat aggtgggccg gttcccgtaa ttcaggacgc ccgggaacca gcgggtctcc
      661 gggatcaccc gggccgaagc aacatagaca ccaaccggca actggatatt cgcgtgcacc
      721 tcgacattgg ctgtcggagc gcccagacgg gccccacggc ccttcccgcg gaccactttc
      781 ccgaaaaggc tgaacggacg accgagacaa tgggacactt tttcgacctt tccctccgaa
      841 acaagttccc ggaggccgga gctcgaaacg gggcggccgc cgatcatgac cggctccatc
      901 ttccggaaca ggaaaccgtg ttttccggca aggtcgcgca tgagatccgt cgtcccgcag
      961 cggcgataac cgaaacgggc gtcatacccc atgcaaacct cgaaaacgcg aagcttcctg
     1021 acaagcacct tctccacaaa gacctcgggc ggcattttcg agaaggcctt cgtgaaggcc
     1081 ggcgcaaaac aaaggtcgat ccccgcctgt ttcagataaa aaagtttctg cccgaccgaa
     1141 aaaaggagcc tttgttcctt ttcgggatgg agtacgatct ggggatggtt acggaacgtc
     1201 agcgccgcgg ctaacgcgtc gtgccgacgg gcctgcgtgg tcacataacg gatcagcttt
     1261 tgatgcccga ggtggaaccc gtcgaagttc ccgagagcca ggaccagccg cctgccgcgg
     1321 ccggccttga acttatgaag gtcggtgact gttcgcatat tgcggcaccg cgccaagcgg
     1381 aagcattttg gggagaagct cttcgaggga catcgtccgg agcgtatcga tcgtcacgct
     1441 tttcgtgatg tcaaaattcc cgctgcagag ccgcctcagt cttgccagac aggcatggca
     1501 gccgaggacc tcgccgagat cgttgacgag ggtccgaaga tacgtgcctt ttggaacatg
     1561 ggctgaaaaa tcggcgaacc gcccctccat tttatcaaaa cggaactcat gaaccgtaac
     1621 gcgacgccct tccctcggaa cggtctttcc ttgccgcgcg agtttgtaga gccgcgtgcc
     1681 ttcgtgcttg agcgcggaga ccatcggcgg aacctggacg atctcgcccg tgaaatcccg
     1741 ggccttttcg cggatcaggt cgatcgtcac gtgttcccag ggcgcttcgg cgatgacctt
     1801 cccctgccgg tcatgggaat ccgtctgtac gccgagctcc atcgttccgt agtattcctt
     1861 gtcgcaagcg ctcagggcca tcgaatattt cgtggccttc ccgatcagga tgacaagaag
     1921 gcccgtggca agcggatcga gcgtgccggc atgaccgact ttcctgaacc tgaaacgttt
     1981 gcggatgaag gcacagacat catgggacgt ccacgtctcg ggcttgtccg caagcaggac
     2041 cccgtcaacg aattccggtc ggataggcat aagctactgt ccggtgggag ggttctcgat
     2101 cttcctgatg agcgcttcga tctcgaaact tttgtcgacc atatcatcga gctcgaactg
     2161 aagatgcggg gtgtaacgca ttgtgagggt cttcccgacc tcccgctgga gaaatccggc
     2221 ggcgcggcgc aggacatcga gggaatcttt cctcttggcg gcatccccca tcacagtgaa
     2281 gaacacgacg gcggatttaa ggtcggccgt taccttcacg tgcgtcagag tcacgaaacc
     2341 gaggcggggg tccttgacgc gctcgaggat gagcttgccg agttccatct ggagaagaga
     2401 gccgacgcgt tctgttcgtt ttccctgcat ggtaggacct gttttttccg gatcagagcg
     2461 tcgcggcgat cttgtcgacg cggtacgact cgatgatgtc gccttcgcga aggtcgttga
     2521 aacccacgag cgaaataccg cactcgtggc cttccgcgac ctctttgacg tcatccttga
     2581 aatgtttgag cgcggagacc cggccgtcaa agacaacgac gttattgcgg acgacccgga
     2641 caccatgatg gcggaccagc cggcccttga tcacccgggc accgccgatc gtgccgacct
     2701 tggaggacgt gaagatctgc cggatctgcg ccttgccttc gatgacctcc tcgagttcgg
     2761 gctcgaggat accttccatg gccttgcgga tcttttccac agcgtcatag atgatgccgt
     2821 aaaaacgtac atccacgccc tctttttcgg caatgccgag ggccttggga tcggccttta
     2881 cgtggaatcc gatcacgatc gcgtccgagg cggcggccaa catgacatcg gactcattga
     2941 tcccgccgac cgcgccatga atgacgtgga gacggcattt ttcggtcgtg agtttttcca
     3001 gcgattgcgt gagggcctcg accgaacctt gggcgtccgc tttcacgatc agcttgagct
     3061 ccttaaagct cccttcggag attttggagt acaggctttc cagggacatg tgcttgctga
     3121 cgccccgcat ctgccgttcc cggatctcga ggatccgttg ttcggtgatc cgcttcgccg
     3181 ttttctcgtc ctcgacagca tacaacatct caccggcgtc aggaacgccg ttcaaaccgc
     3241 tcaattccac cgcgtaggcg ggccccgcct cgtggacggt cttggcgcgg tcattcttca
     3301 tcacacgcac cctgcccgtg taggatccgg ccaccacgat atcgccggtg cgcagggtac
     3361 ccttcttgac gatcacagtc gcgatcggac cgcttccctt ggaaagatga ctttcgacca
     3421 ctgtcccgac cgccgggcaa tcggggttcg ccttcagttc catgacctct gcctggagga
     3481 gaagcatatc gagaagctcg gggacgcctt taccggtctt cgcggaaacc ttcatgcaga
     3541 tcgttttccc gccccactct tccggcatca ggtcctgttt ctggagtccc cccatgacct
     3601 gttgcgggtt cgcgaccgga aggtcgcatt tgttgatcgc aaccacaatc ggacatcccg
     3661 cctcgcgggc gtgatggatc gcttcgatcg tctgcggcat gacaccgtca tcgccggcaa
     3721 cgaccaggac cacgatatcc gtgacgttcg cgccgcgggc ccgcatcgcc gtgaaggcct
     3781 cgtgaccggg cgtatcaagg aacgtgacat gtcctttccc gggaatatcc acgccatagg
     3841 cgccgatatg ctgggtgatg aaaccctttt cccgctccgc gacgttcgtg ctgcggatcg
     3901 cgtccagcag cgacgttttt ccgtggtcga catggcccat catcgtcaca accggcgggc
     3961 ggagcttcag cctggacttg tcctcggccg cttccgcctt ctggaccttc tcgaccacct
     4021 cgtcttccat cttccggatt tcgacaccca ccgcgtcagc aagggcgaag acgatctcct
     4081 cattgagaag ctggttgacg ttcgcgaaaa taccgagtcc catcagggtc ttgatgacct
     4141 ccgggaccct tttgttcatc ctttgcgcaa ggtcgcccac cgtgatcggg aaccggactt
     4201 cgatcctggg cggttcgacc ttcacgggtt ccggtttcgg cgcgggcgcc ggaaccactt
     4261 tcggggattc gggggcctgg gccttcggtt t
//
LOCUS       DAOOBX010000031         4252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_23381_length_4252_cov_3.577555, whole genome shotgun sequence.
ACCESSION   DAOOBX010000031 DAOOBX010000000
VERSION     DAOOBX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23381_length_4252_cov_3.577555"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLY30_00875"
     CDS             <1..185
                     /locus_tag="PLY30_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64683.1"
                     /translation="PERSSGGFEALKNFCKKTPKNIKEPNRAGGILAPEKAKSIANSK
                     KNFRRKIILARKIRGP"
     gene            260..979
                     /locus_tag="PLY30_00880"
     CDS             260..979
                     /locus_tag="PLY30_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW64684.1"
                     /translation="MKEMNPGVRAYAVTIAFLFFIAVLSVAILVFPDHPAVRVFWGGT
                     YASLIRGALGVTLVLWIVGRFCGRAIPRRWLQWTGGLLFLPVAFFPLLRCYFKVPYVF
                     CRACPHPCPWGISRVFLFNGFLLLNLAGRSWCDLLCPFGTFQEALSRITRRHWRFSAW
                     FAGTAYAALFAALGFYLATLFRWPWLNFFDVGRYDFLKVTALFAVACVVAAFFVPKFW
                     CRYLCPVGTIDSLVSSFRKKA"
     gene            complement(1026..2657)
                     /gene="groL"
                     /locus_tag="PLY30_00885"
     CDS             complement(1026..2657)
                     /gene="groL"
                     /locus_tag="PLY30_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857353.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPW64685.1"
                     /translation="MAAKSLSYSEEARQKIKRGIDILARAVAVTLGPRGRNVVIDRKF
                     GAPLVTKDGVTVAKEIDLKDPYENMGCQMVREVAEKTSDLAGDGTTTATVLAQAIVTE
                     GLKNVTAGANPMALKRGIDKATEKIVESVKKLSKTVKGKEDVKAVGTISANNDASIGE
                     ILADAIEKVGKDGVITVEEAKGMKTELEIVEGMQFDRGYSSPYFATDTERMEVVLKEP
                     YILLYEKKIASVKDLVPMLEKVARLGKPLLIISEDIEGEALATLVVNKLRGVLQIAAV
                     KAPGFGDRRKAMMEDIAILTKGKFISEDLGVKLESVELDMLGTAKRVVIDKDDTTIVG
                     GAGSDKDIKARCEQIKVQIEKSDSDYDKEKLLERIAKLSGGVAVVKVGAATEVEMKET
                     KARVEDAHHATRAALQEGIVPGGGVALLRAIESLDTLKLKGDEATGVQIVRRAIEEPA
                     RKIASNAGFDGSVVVKKILEGKNGFGFNAQTGKFSDLLEDAVIDPAKVVRVAIENATS
                     IAGLILTTECVVAEKPREKKEDEKKPASNYSGSIR"
     gene            complement(2773..3069)
                     /locus_tag="PLY30_00890"
     CDS             complement(2773..3069)
                     /locus_tag="PLY30_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPW64686.1"
                     /translation="MKVKPLADRVLVEILEAEEKTKGGIILPDTAKEEKTEGKVIAVG
                     AGKMLESGKIQPVEVKKGDRVLFGKYSGDDIIIDGTKHKILKESEILAVYEGDK"
     gene            complement(3153..>4252)
                     /gene="groEL"
                     /locus_tag="PLY30_00895"
     CDS             complement(3153..>4252)
                     /gene="groEL"
                     /locus_tag="PLY30_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPW64687.1"
                     /translation="LDVVEGMQFDQGYLSPYFVTDAERMEVVLEEPYILINEKKISAV
                     KDLLPILEATAKTGKPLLIIAEEVDGEALATLVVNKIRGTLTVCAVKAPGYGDRRKAM
                     LEDIAILTGGRAITEDLGIKLENVGVKELGRAKRVTVDKENTTIIEGAGKSADIAGRI
                     GQIRKQIEETDSDYDREKLQERLAKLAGGVAVIHVGAATETEMKEKKARVEDSLHATR
                     AAVEEGIVPGGGVALLRAKTVLDTLKLKGDEAIGADIIRRSLEEPLRQLAHNAGDEGS
                     VVVQRVADEKGSTGYNAATGEFENLVEAGIIDPKKVTRSALQNASSVAGLLLTTETLI
                     TDLPEEEKMPPMPGGGMGGMGGMGGMGGMDY"
BASE COUNT          688 a         1245 c         1256 g         1063 t
ORIGIN      
        1 atccggagcg aagttccggc ggttttgaag ctctgaaaaa tttttgtaaa aaaactccga
       61 agaatattaa agaaccgaat cgagcgggtg ggattttagc acccgagaaa gcgaagtcaa
      121 tcgctaattc aaagaaaaat ttccggcgaa aaataatcct tgcgcgaaag atccgcggac
      181 cgtgaaaatc cggaaaaatc gtgatatgct tggcgtgccg gcgaagattc ttcgggaaaa
      241 aacggcaggg ggaaggaaaa tgaaagagat gaatccgggg gtgcgcgctt acgcggtcac
      301 tatcgccttt ctttttttta tcgcggtcct ttccgtggcg atcctcgtct ttcccgatca
      361 ccccgcggtt cgtgttttct ggggcgggac gtacgcttcc cttatccggg gggctttggg
      421 cgtcacgctt gtcctctgga ttgtcggaag gttctgcgga agagcgatcc cgcgccgctg
      481 gctgcaatgg accggcggtc ttcttttcct gccggtcgct ttcttcccgc ttctcaggtg
      541 ttatttcaag gtcccctacg ttttttgccg cgcgtgtcct catccgtgcc cgtgggggat
      601 ctcaagggtg ttcctgttca acggtttcct gctgctgaat cttgccggga ggtcgtggtg
      661 cgatttgctc tgcccgttcg gcactttcca ggaggcgttg tccaggatca cgcggcggca
      721 ttggcggttt tccgcgtggt tcgccgggac ggcgtatgcc gcgctctttg cggcgctcgg
      781 gttttatctc gcgacgcttt tccggtggcc gtggttgaat tttttcgatg tggggcgtta
      841 cgattttctc aaggtaacgg ccttgttcgc ggtcgcatgt gttgtcgcgg cgttctttgt
      901 gccgaaattc tggtgccgtt atctttgtcc ggtcggcacg atcgattcgc tggtctcaag
      961 tttccggaaa aaggcataaa aaaaccccgc tcccgcttgc gcgggaacgg ggtttttcga
     1021 accgcttacc ggatgctgcc ggaatagttc gaggccggtt ttttctcgtc ctctttcttt
     1081 tccctgggtt tctccgcgac cacgcattcg gtcgtgagaa tgagaccggc gatcgaggtc
     1141 gcgttctcga tcgcgacgcg aacgaccttg gccgggtcga tgaccgcgtc ctcaagaaga
     1201 tccgagaact tgccggtctg ggcgttgaaa ccgaaaccgt tcttgccctc gagaatcttc
     1261 ttcacgacaa ccgagccgtc aaaaccggcg ttagaagcga tcttgcgggc cggttcctcg
     1321 atcgcgcgcc gcacgatctg cacgcccgtt gcctcgtcgc ctttcagctt gagggtgtcg
     1381 agcgattcga tggcgcggag cagcgcgacg ccgccgccgg ggacgatccc ttcctggagc
     1441 gccgcgcggg tggcgtgatg agcgtcctca acgcgggcct tggtctcttt catctcaacc
     1501 tcggtggccg cgccgacctt cacgacggcg acaccgccgg aaagtttcgc gatgcgttcc
     1561 aggagcttct ccttgtcgta gtccgaatcg gatttctcga tctggacctt gatctgttcg
     1621 cagcgggcct tgatgtcctt gtcggaacct gcgccaccga cgatcgtggt gtcgtcctta
     1681 tcgatcacga cccgtttcgc ggtgccgagc atatcgagct ccacgctctc gagctttacg
     1741 ccgagatcct cggagatgaa tttccccttg gtcaggatcg cgatatcctc catcatcgcc
     1801 ttgcggcggt cgccgaaacc gggtgccttg acggctgcga tctggagcac gccgcggagc
     1861 ttgttgacca cgagtgtcgc gagcgcctca ccctcgatgt cctcggagat gatgagaagc
     1921 ggtttgccga gacgcgcgac cttctcgagc atcggcacga ggtccttgac ggaggcgatc
     1981 ttcttttcat aaagaaggat gtatggctcc ttcaggacga cctccatccg ctcggtgtcc
     2041 gtcgcgaaat acggggacga ataaccgcgg tcgaactgca tgccttcgac gatctcaagt
     2101 tcggtcttca tgccttttgc ctcttcgacc gtgatgacgc cgtccttgcc gaccttctcg
     2161 atcgcgtcgg cgagaatctc accgatcgaa gcgtcattgt tggcgctgat cgtgccgacg
     2221 gctttgacgt cttccttgcc cttgacggtc ttggagagct tcttcacgga ttcgacgatc
     2281 ttctcggtgg ctttgtcgat gccgcgcttg agagccatcg ggttcgcgcc ggccgtgacg
     2341 ttcttcaggc cttcagtcac gatggcctgg gcgagtaccg tcgcggtcgt cgttccgtcg
     2401 ccggcgagat cggaggtctt ttcggcgacc tcgcggacca tctggcatcc catattctcg
     2461 tacgggtcct tcaggtctat ctccttggcg accgtgacgc cgtcctttgt gacgagcggg
     2521 gcgccgaact tgcggtcaat gacgacgttc cggccgcgcg gaccgagagt gacggcgacg
     2581 gcccttgcga gaatgtcgat gccacgcttg atcttctgac gcgcttcttc ggaataggaa
     2641 agtgatttag ctgccataga ttgactcctt ttttagactg cccaatttct aaatccgaat
     2701 gacgaaacaa tacctgaaga cctgatcccg gacaaaccct cggtccggca ttcgatattc
     2761 ggatttggaa ttttacttgt caccctcgta aaccgcgagg atctcgcttt ctttcaggat
     2821 tttgtgtttc gtgccgtcga tgatgatatc gtcgccggaa tatttcccga aaaggacgcg
     2881 gtcgcccttc ttcacttcga cgggctggat cttgccggat tcgagcattt ttccggcgcc
     2941 gacggcgatg accttccctt cggtcttttc ctcctttgcc gtgtccggga ggataatacc
     3001 gcccttggtc ttttcctcgg cttcgaggat ttccacgaga acgcgatctg ctaacggctt
     3061 tactttcatt gtctgatctc cttatgattg atgtggttaa gaaccagagt caacccgcgc
     3121 ggttgagttt ttaaccgcgc gggttgggga ggttaatagt ccatcccgcc catgccgccc
     3181 attccaccca tcccgcccat gccgccaccg ggcatcgggg gcatcttttc ctcttcgggg
     3241 agatcggtga tcagggtttc ggtggtgaga aggagtcccg cgacgcttga ggcgttctgg
     3301 agcgcgctgc gggtgacctt cttcgggtcg atgatgccgg cctcgacgag gttctcgaac
     3361 tcaccggtcg cggcgttgta gcccgtgctg cctttttcgt ccgcgacgcg ctgaacgacg
     3421 accgaccctt cgtcacccgc gttgtgcgcg agctggcgga ggggttcctc gagcgagcgg
     3481 cggatgatat cggcgccgat cgcctcatcg cccttgagtt tcagggtgtc gagaacggtt
     3541 ttggcgcgca agagcgccac gccgccgccg gggacgatgc cttcttccac ggccgcgcgg
     3601 gtcgcgtgca gggaatcctc gacgcgcgct ttcttttcct tcatttcggt ttcggtcgcg
     3661 gctccgacat ggatgactgc cacgccgccc gcgagtttcg cgaggcgctc ctggagcttc
     3721 tcccggtcgt aatcggagtc cgtttcctcg atctgtttgc gaatctggcc gatgcggccc
     3781 gcgatgtccg cgctcttgcc ggcgccttcg atgatcgtgg tgttctcttt gtccacggtg
     3841 acgcgtttcg cacggccgag ttccttcacg ccgacgttct cgagcttgat accgaggtcc
     3901 tcggtgatcg cccgtccgcc cgtgaggatc gcgatgtcct cgagcatggc cttgcggcga
     3961 tcgccgtagc cgggggcctt cacggcgcag accgtaagcg tcccgcggat cttgttgacc
     4021 acgagggtcg cgagcgcttc gccgtcaact tcttcggcga tgatgaggag gggtttgccg
     4081 gtcttcgcgg tcgcttcaag gatcggaagc aggtccttca ccgcggagat cttcttttca
     4141 ttgatgagga tataaggttc ttcaagcacg acctccatgc gctccgcgtc ggtgacgaag
     4201 tagggggaga gatagccctg gtcgaactgc atgccctcga ccacatccag cg
//
LOCUS       DAOOBX010000032         4224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_23559_length_4224_cov_2.482850, whole genome shotgun sequence.
ACCESSION   DAOOBX010000032 DAOOBX010000000
VERSION     DAOOBX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23559_length_4224_cov_2.482850"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..250
                     /locus_tag="PLY30_00900"
     CDS             26..250
                     /locus_tag="PLY30_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64688.1"
                     /translation="MEKKKESHKSPCCCCSALAGVLVIVFAWWNVSWAPIALTVLGVA
                     VIVKELTGCICKRDVCKKLDKTNPESDNQT"
     gene            410..1222
                     /gene="dapF"
                     /locus_tag="PLY30_00905"
     CDS             410..1222
                     /gene="dapF"
                     /locus_tag="PLY30_00905"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPW64689.1"
                     /translation="MRKTIPFYKMVASGNDFVVVDNRRGVVKNAAAFSKRVCELHQGV
                     GADGVLLYEKSAKADFRMRIINLDGSEAAACGNGFRCIALYAREVIKLPPKMIFESGS
                     GLIEAAVGQSGDVKVQLVKPRIFEMSGMLEVSGHRLHYAFVDTGVPHIVIFVEGLGKI
                     DVATVGRAVRTHAKFKPAGTNVNFVEVKSKREIMVRTYERGVENETLACGTGSTASSV
                     VSVLKGYVATPVTVHTRGGEKLVIDLERKGTVVTKAFLQGKAQVVYRGEYFL"
     gene            1247..2131
                     /gene="dapA"
                     /locus_tag="PLY30_00910"
     CDS             1247..2131
                     /gene="dapA"
                     /locus_tag="PLY30_00910"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPW64690.1"
                     /translation="MAKKKIRFAGSMVALVTPFRSGKVDRKAMAGLIDFHLKAGTDVI
                     IPCGTTGESATMSHEEHRDTMSFVVDYVNGRVPVIAGCGSNNTAEAFGLVQHAKRTGA
                     DAALIVTPYYNKPTQEGLYRHYEYLAGRVDIPIVLYNVPGRTGVSIAPETVARLSKID
                     NIVAIKEASGSMDQADQIMQLCDLPVISGEDSLTFPLIAAGAVGVISVSANVAPKLVH
                     DMVWAALDGNMAKARELHKKHYPLSKAVFIETNPIPAKTALGMMGMIKPELRLPLCEM
                     KPENVKKLRAVLKTLKIV"
     gene            2176..2979
                     /gene="dapB"
                     /locus_tag="PLY30_00915"
     CDS             2176..2979
                     /gene="dapB"
                     /locus_tag="PLY30_00915"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPW64691.1"
                     /translation="MIRLAVAGAAGRMGKIILKLAAKDRAFRITGGIEQRGHATLDMD
                     IGSLIGGDLLNVRIDSSPVAVMKTSDVLIDFSHFSALPDNLAACVAAKKKYVIGTTAI
                     PPGMMKKIALASKKIPIVQSPNMSVGVNLLFRLAAFAGKVLDESYDLEIMEIHHRMKK
                     DAPSGTALKLAEILADARGRDPAKDLVCGREGDTGARQQGKIGVFAMRGGDVVGEHTV
                     SFFGDGERVELVHKATSREALARGALLAAKFLAKKKTGLYHMGHVLGMM"
     gene            3045..>4224
                     /locus_tag="PLY30_00920"
     CDS             3045..>4224
                     /locus_tag="PLY30_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64692.1"
                     /translation="MILKNFIPPVLASAVLFCSSPAFAGILTTEPEAAPAETPGRNLY
                     AEHFYAPSGQKLDLKVSGVRGFPVIKTRSRPQNAEYSIVTDFGGGALRSRFLPGLKDI
                     KAIDEYIKKNLQVANFEGVEIRKLPLPLAGGGTSDLFWVGDKAFPTAAQATAEVTRVK
                     SRIESQGGNFAKSVQAVPVFVAPEKPEPVKTKAQYQKEEELVLKFADQLDLGEKMWGP
                     FQGEPAGEPVLWQSFGETTYRMTNLENRNFNSQVGYWTNRIVLKGIRAPLSTIDPFFE
                     ETTTIEAVGADYKSHFDIFAGFEWRPFARNPWLYNFRPFGWDIPLLEWVRNYKFYIKY
                     GNRYNIKDEIQNAKDYDLVAGVQIFYEWGTELPPLDEGKPERFADYLRQYVWGEYFGD
                     Y"
BASE COUNT          935 a         1134 c         1314 g          841 t
ORIGIN      
        1 gcttaaccga gaaagggagg gggtcatgga aaaaaagaaa gaaagtcaca agagtccgtg
       61 ttgttgctgt tccgcgctcg ccggagtgct tgtgatcgtg ttcgcctggt ggaatgtctc
      121 ctgggccccg attgccctga ccgtgctagg tgtggccgtc attgtgaagg agttgacggg
      181 atgcatctgt aagagggacg tgtgcaagaa actcgataaa acgaatcccg aaagcgacaa
      241 tcagacctga tttgtcccgc cttcgcgaag ttttgatccg ggggcccgga aagaggccgc
      301 aaaactcttc gtgcggttcc ccggctcctt tccaatcaat ccttttggat tcccgggggg
      361 gatttgatag aatacgcccg cctaaccgca ggtttatcaa gacgttatca tgagaaaaac
      421 aatacccttc tataaaatgg tcgcctcggg gaacgatttc gtggttgtgg acaaccgacg
      481 gggggtcgta aagaacgccg ccgctttctc gaagcgggtc tgcgagcttc atcagggagt
      541 gggcgcggac ggcgtcctgc tttatgaaaa gtccgcgaag gcggatttta ggatgcggat
      601 catcaacctg gacggttccg aggccgcggc ctgcgggaac ggtttccgtt gtattgccct
      661 gtatgcgcgc gaagttatca aactgccccc aaagatgatc tttgagtcgg gctccggact
      721 cattgaggcc gcagtcggac aaagcggtga cgtgaaggtc cagcttgtga agccgaggat
      781 ttttgagatg agcggcatgc ttgaggtctc cggccaccgg ctccactacg cgttcgtcga
      841 caccggtgtc ccgcatatcg tgatctttgt ggaggggctc gggaagatcg atgttgccac
      901 cgtcggacgg gcagtccgca cgcatgcgaa gttcaagccg gcgggaacga acgtgaattt
      961 tgtcgaggtg aagagcaagc gcgagatcat ggtccggacg tacgagcgcg gtgtcgagaa
     1021 cgagacgctc gcgtgcggga cgggatcgac ggcttcttcc gtcgtgagcg tgctcaaggg
     1081 gtatgtcgcg acaccggtca cggtccacac gaggggcggc gagaagcttg tgatcgatct
     1141 ggagcgcaaa ggtaccgtcg tcacaaaggc cttccttcag gggaaggcgc aggttgtcta
     1201 tcggggcgaa tattttcttt aatcaggcag ggagaaggaa gagaacatgg ctaaaaagaa
     1261 aataaggttc gcgggttcca tggttgcgct tgtcacgccg ttcaggagcg gtaaggtcga
     1321 tcgcaaggcg atggcggggc tcatcgattt ccacctgaag gccgggacgg acgtgatcat
     1381 tccctgcggc acgacggggg agtccgcaac gatgtcgcat gaggaacacc gtgacacaat
     1441 gtcgttcgtc gtcgattacg tgaacgggcg cgtccccgtg attgcggggt gcgggtcgaa
     1501 caataccgcg gaggcgttcg gccttgtcca gcatgcgaag cgaaccggcg cggacgcggc
     1561 gctgatcgtg acgccgtact ataacaagcc gacgcaggaa ggtctctacc gccactacga
     1621 atacctcgcg ggccgggttg acatcccgat cgtcctttat aacgtgccgg ggcgtaccgg
     1681 agtgtcgatc gcgcctgaga ccgtggcgcg gctttcgaag atcgacaata ttgtcgcgat
     1741 caaggaagcg agcgggagca tggaccaggc ggaccagatc atgcagctct gcgaccttcc
     1801 cgtcatttcg ggcgaggaca gcctgacctt cccgctgatc gcggccgggg cggtgggtgt
     1861 gatctcggtg tcggcgaacg tcgcgccgaa gctcgttcat gacatggtgt gggcggcgct
     1921 cgacggcaat atggcgaagg cgcgggaact ccacaagaaa cactatcctc tttccaaagc
     1981 ggtcttcatc gagacgaatc ccattccggc caagacggcg ctcgggatga tggggatgat
     2041 caagccggag ctgcggcttc cgctctgcga gatgaaaccg gagaacgtga agaaactgcg
     2101 ggcagttctc aaaacgttga aaatcgttta accaaaatcc aacctcgtag gttggagtgg
     2161 tataaggaga agggtatgat cagattggcg gtggcgggcg cggcgggcag gatgggaaag
     2221 atcatcctga agctcgctgc gaaggacagg gcgttcagga tcacgggcgg catcgaacag
     2281 cgcggccacg cgacactgga catggatatc ggttccttga tcggcggcga tctccttaat
     2341 gtccggattg attccagtcc ggtcgcggtc atgaagacgt cggatgtcct gatcgatttc
     2401 agtcattttt cggcgctgcc ggataacctt gcggcctgtg tcgcggcgaa gaagaagtat
     2461 gtgatcggga ccaccgcgat tcctcccggg atgatgaaaa aaatcgccct cgcttcaaag
     2521 aagataccga tcgtccagtc gcccaacatg agcgtggggg tgaacctcct gttccgcctc
     2581 gcggcgttcg cgggcaaggt cctcgacgaa agctacgacc tcgagatcat ggaaatccac
     2641 caccggatga aaaaggacgc gccgagcggc accgctctta aactggccga aattctggcc
     2701 gatgcaagag gcagggaccc ggcgaaggat ctggtctgtg gccgcgaagg ggacaccggc
     2761 gcccggcagc aagggaagat cggcgtattc gccatgcggg gcggcgacgt ggtcggtgaa
     2821 catacggtgt cgtttttcgg ggatggggag cgtgtcgaac tggtccacaa ggctacttcc
     2881 cgcgaagctt tggcccgggg cgcgcttctg gcggcaaaat tccttgcgaa aaagaagacg
     2941 ggtctctatc atatgggcca tgttctcgga atgatgtaag ttttggcggg accttgttca
     3001 ttaaagtttt gcaaaagccg aaagccaaaa actaaaaatt tttaatgata cttaaaaatt
     3061 tcataccccc tgttttggca agcgcggttc ttttctgttc ctcccctgcg tttgcgggca
     3121 tcctgacgac cgagcctgaa gccgctccgg ccgaaactcc cgggagaaat ctctacgccg
     3181 agcatttcta cgccccgtcc ggccagaaac tcgacctgaa ggtctccggg gtccgcggct
     3241 ttcccgtgat caagacccgc tcgcggccgc agaacgccga atattccatc gtgacagatt
     3301 tcgggggcgg cgcgctccga tcgcgtttcc ttcccggcct gaaagacatc aaggcgatcg
     3361 acgaatacat caagaagaac ctgcaggtcg cgaatttcga aggcgtagag atccgcaaac
     3421 ttcccctccc gctcgcgggc gggggaacgt ccgatctttt ctgggtcggg gacaaggcct
     3481 tcccgacggc cgcccaggcg accgcggaag tgacccgcgt caaatcgagg atcgaatccc
     3541 agggcgggaa tttcgcaaag agcgtccagg ccgtgccggt gtttgtcgcg cctgaaaaac
     3601 cggagcccgt gaagacgaag gcccagtacc agaaggaaga agagctcgtt cttaaattcg
     3661 cggaccagct cgatctcggg gaaaagatgt ggggcccctt ccagggcgag ccggccggcg
     3721 aaccggtgct gtggcagtcc ttcggcgaaa caacctaccg catgaccaac ctcgagaaca
     3781 ggaacttcaa cagccaggtc ggctactgga cgaaccgcat cgtccttaaa ggcatccgcg
     3841 ccccgctcag caccatcgat cccttcttcg aggagacgac aacgatcgaa gcggtcgggg
     3901 cggattacaa gagccacttc gatatcttcg ccggattcga atggcgtccc ttcgcgcgca
     3961 atccctggct ttacaacttc cgcccgttcg gctgggacat cccgctcctt gaatgggtcc
     4021 ggaattacaa gttctatatc aaatacggca accgttacaa catcaaggac gagatccaga
     4081 acgcgaaaga ctacgatctg gttgccgggg ttcagatttt ctatgaatgg ggcacggagt
     4141 tgccgcctct ggacgaagga aagcccgaac gttttgccga ttatctccgc caatatgtgt
     4201 ggggcgaata tttcggcgac tacc
//
LOCUS       DAOOBX010000033         4210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_23656_length_4210_cov_3.392780, whole genome shotgun sequence.
ACCESSION   DAOOBX010000033 DAOOBX010000000
VERSION     DAOOBX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23656_length_4210_cov_3.392780"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /gene="hisC"
                     /locus_tag="PLY30_00925"
     CDS             complement(<1..990)
                     /gene="hisC"
                     /locus_tag="PLY30_00925"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPW64693.1"
                     /translation="MKMIPFKPNIQSIRPYEPGKPIKELQREYGIRNVTKLASNENPL
                     GRPSTDVLRAMRKAIREVHLYPDGSCYYLLERLAGEIDIPKDQIIFGNGTNEIIELVA
                     RGFLSEGDEVISSEMSFLVYPILTQVCGGRFKAVPMTKDYRYDLDGILAQITERTKLV
                     FIVNPNNPTGTYLTAREMDAFLAKVPPRVIVCFDEAYVDFVEAADFPRSLSYIREGRT
                     NVIVLRTFSKAYGLAGLRVGYAVTSREMTEYLHKVRQPFNVNLVAQAAAVAAIEDKFY
                     LWKTKRLVKTGRKYFYKKFKQLGLEYLPSQANFVLVNVKRSGSEIFQELLRSGM"
     gene            complement(1052..2152)
                     /gene="pheA"
                     /locus_tag="PLY30_00930"
     CDS             complement(1052..2152)
                     /gene="pheA"
                     /locus_tag="PLY30_00930"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPW64694.1"
                     /translation="MTRQRKIMDLTHLRKRIDEIDQKIIGLLNDRTKFVREVGAKKIA
                     AGKAVYAPERESEIYRKIDQLAETKGLPKDALKAIYREIMSAALAMEKPLTIAYLGPE
                     ATFTHLAALSKFGSSVEYRPCISFTEVFREVEMGRADYGVVPIENSIEGAVNHTLDMF
                     IDSDLKICSEIFYEISHSLMTNAPDLKRVRRVYSKAEVFGQCRMWLETNLHHAGLVEA
                     PSTAAAAQRAVQEDGAAAIGSKLAATINNLPVLAEAIQDIANNVTRFLVISKQLPAMT
                     KHDKTSVVVSIRDKVGALYEMLLPIKKHKINMTKIESRPSKKKAWDYYFFIDFEGHVE
                     NPRVRKMLSEMERKVKFVKVLGSYPATGEPKD"
     gene            complement(2100..3026)
                     /gene="scpB"
                     /locus_tag="PLY30_00935"
     CDS             complement(2100..3026)
                     /gene="scpB"
                     /locus_tag="PLY30_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPW64695.1"
                     /translation="MSTAAKEREKQAKMKALRKELDREIQDQLEESGVLETLVDPSRY
                     RVESLREEDLKDPASAKKVVEALIFASPKPLTPAEIRKVTKVLTAAQIDQIVTELKEE
                     YKQQGRPFEIVEIAGGYELSTKKEFAPWILRIELQRKARQATQSALETLAILAYRQPL
                     TRAEIEALRGVDVSGVVSTLMEKGFIRIVGKKEVPGRPFLYGTTEKFLEHFGLKSLQE
                     LPSLSEIQQMVESSVKKEQLIGTTRIVDVPQEGVSREGASRDASESAPAHGEISPEEA
                     SESDPGDENAAPVDNNDEATKDHGSDAPAQEN"
     gene            complement(3023..>4210)
                     /locus_tag="PLY30_00940"
     CDS             complement(3023..>4210)
                     /locus_tag="PLY30_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPW64696.1"
                     /translation="LPGDGTPAVNAAGSLPNVSDPDPLPCETGGAEPSGENLSDPSLP
                     KEEESRDPLHPVVDSGDQSSANEQDHGDEVAPDGSTAGDVSPEMESSPGPSVTREEGT
                     PEPAPVAGTTDSAFDPLRVRLPVYDGPLDLLLDLIKKNEMNIYDIPIAEITKQYLDYL
                     HRMKQLDLEVAGEFVVMAATLIYIKSRTLLPTDKEVEENEGEDPRAELVKKLLEYQAF
                     KEAARELGLLQTERGKMFTRQISDYYLSEMKPEDAGIDTFSANFFDLVVAFQNVLAKK
                     EREKPHEVFEEVISIEEKMVEIQAVLIEKNKMRFTELFSGRWSRNELVATFLAILEIV
                     RTRFARVFQDKTFGEIVIEKRDESGYKSTSQNKPAAENAEPPVAGTDEPQPTDTGNHT
                     IGS"
BASE COUNT          798 a         1267 c         1151 g          994 t
ORIGIN      
        1 catgccgctg cggagcagtt cctggaatat ctcactgccg cttcttttca cgttgacgag
       61 gacgaaattc gcctggctcg gcaggtattc aagtccgagc tgtttgaatt tcttgtaaaa
      121 atatttccgt ccggtcttga cgagccgttt ggtcttccag aggtagaact tgtcctcgat
      181 tgccgccacc gcggcagcct gcgcgacgag attcacattg aagggctggc gaaccttatg
      241 gaggtattcc gtcatctccc ttgatgtcac ggcataaccc acgcggagcc ccgcaaggcc
      301 gtaggccttc gagaaggtcc tgaggacgat cacgttcgtc cggccctccc tgatgtagga
      361 aagcgacctc ggaaaatcag cggcctcgac aaaatccaca taggcctcgt caaaacagac
      421 gatgacacgc ggagggacct tcgcgagaaa agcgtccatc tcccgggcgg tcagataggt
      481 tcccgtcggg ttgttcgggt tcacgataaa gacaagcttc gtccgttccg tgatctgggc
      541 caggatgccg tccaggtcgt aacggtagtc tttcgtcatc ggaacggcct tgaaccggcc
      601 gccgcaaacc tgcgtcagga tcggatagac caggaacgac atctcggagg aaatgacctc
      661 atccccttcc gacagaaaac cgcgggccac cagttcgatg atctcgttgg tgccgttgcc
      721 gaaaatgatc tggtccttcg ggatatcgat ttccccggca agccgttcga gcaggtaata
      781 gcagcttccg tccggataaa gatgaacctc gcggatcgcc ttgcgcatgg cgcgcaggac
      841 gtcggttgag ggacgcccga gcgggttctc gttcgaggcc agcttcgtga cgttgcgaat
      901 cccgtactca cgttgaagct ccttgatggg cttccccggt tcgtagggac ggatactctg
      961 gatattcggc ttaaagggta tcattttcat atcacacatc acacgccaca aggccacatg
     1021 catcgcgcgg cggacggcgt gcgccttatt tttagtcttt cggttctccc gtcgcgggat
     1081 aggagccgag cactttcacg aacttgactt tccgctccat ctccgaaagc atcttccgga
     1141 cccgcgggtt ctcaacgtgg ccttcaaaat cgataaaaaa gtaataatcc cacgcctttt
     1201 tcttcgaagg gcgggactcg atctttgtca tgttgatctt gtgtttcttg atcgggagca
     1261 gcatctcgta aagcgccccc accttgtcgc ggatcgacac gacaaccgaa gtcttgtcgt
     1321 gcttcgtcat cgccggaagc tgcttcgaga tcacgaggaa ccgcgtcaca ttgttcgcga
     1381 tatcctggat cgcctcggca aggacgggca ggttgttgat cgtcgccgcg agttttgacc
     1441 cgatggcggc cgccccatct tcctgtaccg cgcgctgcgc cgccgcggcc gtgctcggag
     1501 cttccacgag ccccgcgtga tgcaggttcg tctccagcca catccggcac tggccgaaca
     1561 cctcggcctt ggagtagacg cgccggacgc gtttgagatc cggggcattc gtcatgaggc
     1621 tgtgcgaaat ctcgtagaag atctcggagc agatcttcag atcggaatcg atgaacatat
     1681 cgagcgtgtg gttcaccgcg ccttcgattg aattctcgat cgggacaacc ccgtaatccg
     1741 cgcggcccat ctcgacctcg cggaagactt ccgtgaagct gatacagggc cgatactcaa
     1801 cgctcgaacc gaacttggaa agggcagcca gatgcgtgaa ggtcgcctcg ggtccgaggt
     1861 aggcgatcgt gagcggcttt tccatcgcga gcgccgccga catgatctcc cggtagatgg
     1921 ccttcagggc atccttgggg agcccctttg tttctgccag ctggtcaatc ttgcgataga
     1981 tctcggactc gcgttcaggc gcgtaaaccg ccttccctgc cgcgatcttt ttcgcgccga
     2041 cttcccggac aaatttcgtg cggtcgttca gaagaccgat gatcttctgg tcgatctcgt
     2101 caattctctt gcgcaggtgc gtcagatcca tgatctttcg ttgcctcgtc attgttgtcg
     2161 accggtgccg cattctcgtc cccgggatca gattccgatg cttcctcagg ggatatttct
     2221 ccatgagccg gcgcgctttc ggaagcatcc cgggaagctc cctcccgaga cacaccttcc
     2281 tggggaacgt ccacaatcct ggtcgtgccg atcagctgtt cttttttcac cgaactttcc
     2341 accatctgct ggatctcgct caggctcgga agctcctgta gtgatttgag cccgaagtgt
     2401 tcgagaaact tttcggtcgt gccgtaaagg aatggccggc ccgggacttc cttcttgccg
     2461 acgatccgga tgaatccctt ttccatcagc gtgctgacca cgccgctcac atccacgccg
     2521 cgaagcgctt cgatctcggc ccgggtcaac ggctggcggt acgcgaggat cgcgagcgtc
     2581 tcgagcgccg actgggtcgc ctgtctggcc ttgcgctgga gctcgatgcg gagaatccac
     2641 ggcgcgaatt ctttcttcgt ggaaagctcg taacctcccg cgatttcaac gatctcgaac
     2701 gggcgaccct gctgtttgta ttcttccttc aactcagtca cgatctggtc gatctgcgcg
     2761 gcggtcagca ccttcgtcac cttgcggatt tcggcgggcg tcaggggttt tggcgaagcg
     2821 aagatcaagg cctccaccac ctttttcgcg gatgcgggat ctttcaggtc ctcctcgcga
     2881 agcgactcga cccgatacct ggatggatcc acgagggtct caaggacccc gctttcctca
     2941 agctgatcct ggatctccct gtcgagctcc ttgcggagcg ctttcatttt cgcctgcttt
     3001 tcgcgttctt tggccgcagt gctcatgacc cgatcgtatg attccccgtg tctgtcggct
     3061 gcggttcatc cgtccccgca acgggaggct cggcgttctc cgcggccggt ttattttgtg
     3121 aagtgctttt atacccggac tcgtcgcgct tttcaatcac gatctcacca aaggtcttgt
     3181 cctggaacac tctggcgaac cgtgtcctga caatttcgag gatggcaaga aacgtcgcaa
     3241 ccagttcatt ccggctccat ctgccggaaa aaagttccgt gaaccgcatc ttgttctttt
     3301 cgatcagaac agcctggatc tcgaccattt tctcctcgat cgagatgacc tcctcgaaaa
     3361 cttcatgcgg cttttcgcgc tcctttttgg cgagcacgtt ctggaacgcc acgaccaggt
     3421 caaaaaagtt cgcgctgaac gtatcgatgc cggcgtcctc gggtttcatc tcgctgagat
     3481 aataatccga aatctgacgc gtgaacatct tgccgcgctc cgtctggagg aggccgagct
     3541 ccctggccgc ttccttgaaa gcctgatact cgagaagctt cttcacaagt tcggcgcgcg
     3601 ggtcctcgcc ctcgttctcc tccacttcct tgtcggtcgg caggagcgtc ctcgatttga
     3661 tgtaaatgag cgttgccgcc atcacgacaa actcccccgc gacttcgagg tcgagttgct
     3721 tcatccggtg gagataatcg agatactgtt tcgtgatttc cgcgatcggg atatcgtaaa
     3781 tattcatctc gttcttcttg atcagatcaa gaagaaggtc aagcggtccg tcatagaccg
     3841 gaagtctgac ccggagcgga tcgaacgcgc tgtctgtggt cccggccacc ggcgcaggtt
     3901 ccggcgttcc ttcttcccgg gttacggaag ggccgggaga gctttccatc tccggcgaga
     3961 catccccggc agtcgaaccg tcaggagcca cctcatcccc atgatcctgt tcattggcag
     4021 aagactgatc gccagaatcg acgaccggat gtagcggatc acgagattcc tcttctttcg
     4081 gcaaggaagg atcggaaagg ttctcacccg aaggctccgc tccccccgtt tcgcaaggga
     4141 gagggtcagg atcggaaacg ttcggcaacg atccggccgc gtttacggca ggagtcccat
     4201 cgcccgggag
//
LOCUS       DAOOBX010000034         4176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_23898_length_4176_cov_3.114778, whole genome shotgun sequence.
ACCESSION   DAOOBX010000034 DAOOBX010000000
VERSION     DAOOBX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23898_length_4176_cov_3.114778"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /gene="lpxC"
                     /locus_tag="PLY30_00945"
     CDS             complement(<1..1182)
                     /gene="lpxC"
                     /locus_tag="PLY30_00945"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPW64697.1"
                     /translation="MKQRTLKSSFGFEGIGLHTGRKVTVKVNPAPANSGILFSRVDLD
                     PALKVRASHENVRSDEMRQTALVVGTGLVRTIEHLMSAFHALGVDNAWIEIDGEEVPG
                     MDGSAQEFTRLILDAGFEEQNAERNFLEIKEPVFVDHGDQSIMIVPAPDLQVSYTLSY
                     HDADLEDQYMTVTILPEIYERELAPARTFCLKKEAEQLLAMGFGKGANPSNTLIFERN
                     RPVQNELRFANEACRHKIVDILGDLFLSGKFIRGHVIAQRTGHSLNLELVRKLAMTLD
                     PNADLMSKKTLTVEEIQKIIPHRYPFLFVDRIENFEPGLRATGWKQVTMNDYFFQWHF
                     PGHPVMPGVLIVEAMAQVGGVIMLGEPENRGKIAYFMSVDQVKWRSPVKPGDSLRMEV
                     EV"
     gene            complement(1192..1704)
                     /locus_tag="PLY30_00950"
     CDS             complement(1192..1704)
                     /locus_tag="PLY30_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPW64698.1"
                     /translation="MKKIFCAFVILTAMFAAMAAPAFAAEMKLAYVDMEEVFDGYQKT
                     KDQDLVLKTAGEAKEKERNDMMASLRSMEDEIALLSADARAKKQEQLIEKKRQLEDFD
                     RNVRQQLAEQRNKMIREIITEIEEAVQAVGQRGGYDMVFNRRMLNYQKKEYDITAQIS
                     AELAKKYKKA"
     gene            complement(1820..4123)
                     /gene="bamA"
                     /locus_tag="PLY30_00955"
     CDS             complement(1820..4123)
                     /gene="bamA"
                     /locus_tag="PLY30_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPW64699.1"
                     /translation="MKKFLSVLLAICLFATGVPSVFAEDAAQTGQSQKKIASVEVRGN
                     KIVSTATILSKIQSQQGAPLRQQQTNEDIKRLYDTGFFQDVRFDVEEISEGYKLIVRV
                     DEKPIIRQIQIQGNTVFKEDKIRKTLGVMEGQILDRKALKKGEEEVRQLYGNKGYRFI
                     DIQTDVSTDAQTKESIVTMKIFEGKKFKIKEIRFEGNQSFQAKQLSKLMKTKAQWWFL
                     FGTFKEEVFEKDIERISFYYQKEGYLDVKVVPEFEYLKDQNQIKILIKIEEGQHYVTG
                     EVKFKGNTLFPESELWQSLEMLPGLTYSQFYVSQDMESIRNFYASEGYIEARVAPDVN
                     LNKETGKVDVVYEITEGELFFVEKIQIRGNTKTRDKVIRRELRIRPGGRFDGKKIEKS
                     KQRLENLGYFEEITYDTEPSDTATNRRDLIFRVKEKRTGELSFGGGVSSIDTFMGFAE
                     ISQRNFDLFNWPRFTGGGQTLALRGQIGTINQQYDFNFVEPYLFDKPVTMDFNTYYTV
                     RDDYNVDFKEKRLGFSDIFSRLFKDVFRVGGGYVLERVNLTDVAGDAPKDVTDFEGVN
                     WLSRIRAFTSFDNRDNVFNPTKGRTINFGGDMVGGFLGGDQDYYILRAAYTEYLSFRK
                     KHMIEFQARVSTAQEFGSSPSVPVFDRFYAGGLGTIRGYGYRRVSPKDNDNPVGGQTL
                     GIVNIDYSFPVPKLDMFRGVVFVDTGFVNPDAYDFGFGDVVVSVGPGIKVKTPIGPLA
                     FYYGFPLVNKDTENENGRFEFSLSRSF"
BASE COUNT          839 a         1221 c         1122 g          994 t
ORIGIN      
        1 cacttcgact tccatgcgga gcgaatcgcc cggtttgacg ggactgcgcc atttcacctg
       61 gtccacgctc atgaaatagg caatcttccc gcggttctcg ggttccccca gcatgattac
      121 gccgccaacc tgcgccatcg cttcaacgat caggacgccc ggcatcacgg gatgtcccgg
      181 gaaatgccac tggaagaaat aatcgttcat cgtgacctgt ttccagcccg tcgcgcgaag
      241 cccgggctcg aaattctcga tccggtcgac aaagaggaac gggtagcggt gcggaatgat
      301 cttctggatc tcctcgaccg tcagggtttt cttggacatc aaatccgcgt tgggatcaag
      361 agtcatggct aatttcctca caagttcaag gttcagggaa tgtccggtcc tctgggcgat
      421 cacatgcccc cggataaatt taccgctcag gaaaagatcg ccgaggatgt ccacgatctt
      481 gtgccggcaa gcctcgttcg cgaagcgcag ttcattctgg accggccggt tacgttcaaa
      541 gatcagcgtg tttgaagggt tcgccccttt cccgaaaccc atcgcgagaa gctgttcggc
      601 ctcctttttc aggcagaagg tcctcgccgg cgcgagttcg cgctcgtaga tctcgggaag
      661 gatggtgacc gtcatatact ggtcttccag gtccgcgtcg tgatagctca gcgtgtagga
      721 aacctgaagg tcgggagccg gcacgatcat gatcgactga tcgccgtggt ccacaaaaac
      781 aggctccttg atctcgagga agttccgttc ggcgttttgc tcttcaaatc cggcgtccag
      841 aatcaaccgc gtaaattcct gcgcgctgcc gtccatgccg ggaacctctt ccccgtcgat
      901 ctcgatccac gcgttatcaa cgccgagggc atggaaagcc gacatcagat gttcgatggt
      961 ccggacaagc ccggtgccga ccacgagcgc cgtctggcgc atttcgtcgg aacggacgtt
     1021 ctcgtgggaa gcccggacct tcagcgcggg gtcaagatcc acgcgcgaaa aaaggattcc
     1081 gctgttggcc ggcgcggggt tgaccttgac cgtgaccttc cgtcccgtgt ggagaccgat
     1141 cccctcgaag ccaaaactgc ttttgagcgt tctttgcttc atggactgcg atcaggcctt
     1201 tttatatttc ttcgcgagtt cagccgaaat ctgggccgtg atgtcgtact ctttcttctg
     1261 gtaattaagc atgcgcctgt tgaacaccat gtcataaccg ccgcgctgac cgaccgcctg
     1321 gaccgcctct tcgatctccg tgatgatctc ccggatcatc ttgttgcgct gttcggcaag
     1381 ctgctggcgg acattgcggt cgaagtcctc gagctgacgt tttttctcga tcagttgctc
     1441 ctgcttcttg gcgcgggcat ccgcggacag gagagcgatc tcgtcttcca tcgaacggag
     1501 gctcgccatc atgtcgttcc gttctttttc cttggcttcg ccggccgtct tcaggaccag
     1561 atcctggtcc ttggtcttct ggtatccgtc aaagacctct tccatatcca cataagcgag
     1621 cttcatttcg gcggcaaacg ccggggccgc catcgccgcg aacatcgccg tcaggatcac
     1681 gaacgcacag aaaatctttt tcattttttc ctcctggtaa gtttatcatt gccactgatt
     1741 gtccggacag gacaagcggt catgccggaa agcgcgtacc gcaccgcggg acggtgtccg
     1801 gaccgtctgc cgcgggatct tagaagcttc ggctaaggct gaactcgaaa cgaccgttct
     1861 cgttctcggt atccttgttt accagcggga aaccgtaata gaaggccagg ggtccgatcg
     1921 gcgtcttgac cttgatacct gggccgacgc tgaccacgac atcgccgaat ccgaaatcat
     1981 acgcatcggg gttcacgaaa ccagtgtcca cgaagaccac accccggaac atgtccagtt
     2041 tcgggaccgg aaagctgtaa tcgatattga cgatccccag cgtctggccg ccgaccgggt
     2101 tgtcgttatc cttggggctg acgcggcgat agccgtaacc gcgaatcgtg ccgaggccgc
     2161 cggcgtaaaa ccggtcaaag accggcacag acggcgaact gccgaattcc tgggccgtgg
     2221 aaacgcgcgc ctggaactcg atcatgtgct tcttcctgaa gctcagatat tccgtatacg
     2281 ccgcgcggag gatgtaataa tcctggtcgc cgccgaggaa gccccccacc atgtctccgc
     2341 cgaaattgat ggtgcgaccc ttcgtgggat tgaaaacgtt atcgcggtta tcaaaactcg
     2401 tgaaggcccg gatgcgggaa agccagttca cgccttcaaa atccgtcaca tccttcgggg
     2461 catcgccggc cacatccgtc aggttgacgc gctcaaggac ataaccgccg ccgacgcgga
     2521 acacatcctt gaaaagccgc gagaagatgt cgctgaaacc gaggcgtttt tccttgaagt
     2581 ccacgttgta atcgtcgcga accgtgtagt aggtgttgaa atccatcgtg accggcttgt
     2641 caaaaaggta cggttcgaca aaattgaaat cgtactgctg gttgatcgtg ccgatctgcc
     2701 cgcgaagcgc gagcgtctgc ccgccgcccg tgaagcgcgg ccagttaaag agatcgaagt
     2761 tcctctgcga aatctcggcg aaccccatga atgtgtcgat cgagctgacg ccgccgccga
     2821 acgagagctc gccggtcctc ttctccttga cgcggaaaat caggtcccgg cgattcgtgg
     2881 cggtatccga cggttcggtg tcatacgtga tttcctcgaa atagccgagg ttttcgagac
     2941 gttgcttgga cttttcgatc tttttcccgt caaaacgccc gcccgggcgg atcctgagct
     3001 cgcggcggat caccttgtca cgggtcttgg tgttgccccg gatctggatc ttctcgacga
     3061 agaaaagctc cccttccgtg atctcgtaga cgacgtcgac cttgccggtc tccttgttca
     3121 ggttcacgtc gggcgcgaca cgggcttcga tatacccttc cgacgcgtaa aagttccgga
     3181 tgctttccat gtcctgcgaa acatagaatt gcgagtaggt caggccgggg agcatttcaa
     3241 gtgactgcca gagctcggac tcggggaaca gcgtgttgcc cttgaacttc acttccccgg
     3301 tcacataatg ctgtccttcc tcgatcttga tgaggatctt gatctggttc tggtctttca
     3361 ggtactcaaa ttccgggacc accttcacgt ccagataacc ttccttctga taatagaagc
     3421 tgatgcgctc gatgtccttt tcgaagacct cttccttgaa cgtcccgaag aggaaccacc
     3481 actgggcctt ggttttcatg agcttggaga gctgtttcgc ctggaaggac tggttgccct
     3541 cgaagcggat ttccttgatc ttaaacttct tgccctcgaa gatcttcatc gtgacgatcg
     3601 attctttcgt ctgggcatcg gtggaaacgt ccgtctggat atcgataaaa cggtacccct
     3661 tgttcccgta aagctggcgg acctcttctt cccctttctt cagggccttg cggtcaagga
     3721 tttgtccttc catcacgccg agcgtcttcc ggatcttgtc ctccttgaaa acggtatttc
     3781 cctgaatctg gatctgacgg atgatgggct tttcatccac ccggacgatc agcttgtaac
     3841 cttcggagat ttcctccaca tcaaaacgga catcctggaa gaagcccgta tcataaaggc
     3901 gtttgatatc ctcgttggtc tgttgctggc ggaggggtgc cccctgctgg gactggatct
     3961 tgctgaggat agtcgcggta ctgacgatct tgttaccgcg aacctcgacc gaggcgatct
     4021 ttttctgact ctgtccggtc tgcgcagcgt cctccgcgaa aacagacggg acaccggtcg
     4081 cgaataaaca gatcgccaac aacacgctca aaaacttctt cattgggctc cttcgttggt
     4141 acggtagagt tccgggttat caaaccgggt ccattc
//
LOCUS       DAOOBX010000035         4174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_23911_length_4174_cov_3.402039, whole genome shotgun sequence.
ACCESSION   DAOOBX010000035 DAOOBX010000000
VERSION     DAOOBX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23911_length_4174_cov_3.402039"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PLY30_00960"
     CDS             complement(<1..1316)
                     /locus_tag="PLY30_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW64700.1"
                     /translation="MTNENPQYPQSGSGPKGGDGLFENTHDLIQLFSPDGILLDVNPS
                     WRRVLERPDDDITQLSVFSVVHPRDHDGFRKTVADVLKGQSPRKIGITMVSQSNREII
                     AEGSVDLIGGHQGSKVLRCILHDITKHRKYDELKDEFVGTISHELRTPLTVVREGMAQ
                     IRDGLLGGVTPAQREVLDMVLQSIDRLGRIINDLLDVSKLESGQVHLKRTLCNMTGIA
                     REILANFEGLASQKGIELRLEAASDVIEVYLDRDKFIQVLTNLVGNAIKFTSVGYVRI
                     SLRELEGFAECKVSDTGRGISGEDLSRVFEKFRQFGRAVGPGIGGTGLGLSICKKLIE
                     LHHGKISIDSYPLKGTSVTVLLPKYTSREIFREHIAQALNRCAEEGGTLSLIIFDIVD
                     FETVKRTLGEGHVREIVAKIDKLMNDSLRRAADVAIRDTKALLILLP"
     gene            complement(1394..2638)
                     /locus_tag="PLY30_00965"
     CDS             complement(1394..2638)
                     /locus_tag="PLY30_00965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW64701.1"
                     /translation="MNYDEYKRKTCRYCETPLGTAFLDLGAQPLANNLPGIENARKPE
                     FRCPLALVKCPECHLVQLTHSVPANLMFDEYLYVSSTTQTFRTHFAEYARDVRERIGP
                     KPGALAVDIGSNDGLLVSSYEKEGLRGVGVEPAKNLAADANTRGVTTLNRYFGEEAVA
                     EIIARYGKADAISANNVFAHIDDIRSVLRNVRELLADDGFFVIEFAYLVTMLEKMYFD
                     MVYHEHVCYIGVTALDLFTTRFGMEIFDIKPVDTHGGSLRVFIQKKGGPRARAGVVGR
                     YLADEKQKGCCEDRVYGEFAERVLGFRSELNGMLDGIKKEGKSVCGYGAPAKATTIVN
                     FCGLTPREIEFIVDDNPLKQGRLVPGAGIPIVPSARLQDVPPDYVLIFAWNFAPEIMK
                     KIGFLRDRGVRFIVPLPRPVLV"
     gene            complement(2705..3433)
                     /locus_tag="PLY30_00970"
                     /pseudo
     CDS             complement(2705..3433)
                     /locus_tag="PLY30_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(3496..>4174)
                     /locus_tag="PLY30_00975"
     CDS             complement(3496..>4174)
                     /locus_tag="PLY30_00975"
                     /inference="COORDINATES: protein motif:HMM:NF016571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPW64702.1"
                     /translation="FCWAIRGYRPKGTCRAGTFKSKVQLAVEILTEAIQALPVKPTVL
                     MDAWYSCAAILNLIIQAGWTFVAAVKRNRLVEINGKKISLSSLAKGPRKYKTIRVSRK
                     KCFRVAKLQVQLPKIGAVLLFISKSRKDGIRFFITNDLKMTESQMVALYLQRVWIETF
                     HQDIKQHLGFGEVFMHSWDGVQAHWALVGIAYNLIALWNGNKSRSFRQMIRHFRDSVD
                     PNTVLGL"
BASE COUNT          862 a         1214 c         1184 g          914 t
ORIGIN      
        1 ggaaggagga tgaggagcgc tttggtgtcc cggatcgcga catccgcggc ccggcgcagg
       61 ctgtcattca tgagcttgtc gatcttcgcg acgatctccc tgacgtgccc ctcgccgagc
      121 gttcgcttga ccgtttcaaa atccacgata tcgaaaatga tgagcgacaa ggtaccgccc
      181 tcttccgcac accgattgag ggcttgagcg atgtgctcgc gaaagatttc gcgcgacgta
      241 tacttgggaa ggagcacggt gacggacgtg cctttcagcg ggtagctgtc gatcgatatc
      301 ttcccatgat ggagttcgat gagttttttg cagatcgaaa gcccgagccc cgtgccgccg
      361 attccgggcc ccacggcgcg tccgaactgc cggaatttct cgaagaccct tgacaggtcc
      421 tcgcccgaaa tgccgcgacc ggtgtccgag accttgcatt ccgcaaaccc ttcgagctcc
      481 cgcagcgaga tcctgacgta tcccacggac gtgaacttga tcgcgttgcc gacgagattg
      541 gtcaggacct ggatgaactt gtcgcggtcg agatagacct cgatcacgtc gcttgcggcc
      601 tcaagcctga gctcgatccc cttctgcgag gcaagccctt cgaagttggc cagaatctcc
      661 cgcgcgattc ccgtcatgtt gcagagggtc ctcttcagat gcacctgacc cgactccagt
      721 ttggaaacat ccagcaggtc attgatgatc cgcccgagac ggtcgatact ctggaggacc
      781 atgtcgagga cctccctctg ggccggcgtc acgccgccga gaaggccgtc ccgaatctgg
      841 gccattcctt ccctcacaac cgtcagaggg gtccgcagtt cgtgggagat ggtcccgacg
      901 aattcatcct tcagctcgtc gtatttccgg tgtttcgtga tatcgtgaag aatacaccgc
      961 aggaccttgc tcccctgatg gccgccaatc aggtcgacgc ttccctccgc gatgatctcc
     1021 cggttggact gggagaccat cgtgatcccg atcttccggg gggattgtcc tttgagtacg
     1081 tctgccaccg ttttccggaa tccgtcatgg tcccgggggt gaaccacgct gaaaacggaa
     1141 agctgagtga tgtcatcgtc cgggcgttcc aggacgcggc gccaggacgg gttcacgtca
     1201 aggaggatgc cgtcggggct aaagagctgg atcaggtcgt gcgtattctc aaaaagaccg
     1261 tccccgcctt tcggacccga accggattgt ggatattgag gattttcgtt cgtcataatt
     1321 tcatgcgtcc cgccccggca ttatcggccc gtccggctcc cttttaaagc gtttcctccg
     1381 ggtaaaacga ccctcagacg agaaccggcc tgggaagagg gacaatgaaa cggacgcccc
     1441 ggtcgcggag gaaaccgatc tttttcatga tctccggcgc gaagttccag gcaaagatca
     1501 ggacatagtc gggggggaca tcctgcaggc gggcgctcgg cacgatcggg attcccgctc
     1561 cgggcacaag ccgtccctgc ttcagggggt tgtcatcgac gatgaattcg atctcccggg
     1621 gcgtcaggcc gcaaaaattg acgatcgtgg tggccttggc gggcgcgccg tagccgcaga
     1681 cggattttcc ttcctttttg atcccatcca gcatgccgtt cagttcggac ctgaacccga
     1741 gaacgcgttc cgcaaactcc ccgtagacgc ggtcctcgca gcagcctttt tgtttctcgt
     1801 ccgcgaggta ccgtccgaca acccccgcgc gcgcgcgggg gccgcctttt ttctggataa
     1861 aaaccctgag ggaaccgccg tgggtgtcga ccggcttgat gtcgaaaatt tccatcccga
     1921 agcgggtcgt aaaaagatcg agggccgtca ccccgatata gcagacatgc tcgtgataga
     1981 ccatgtcgaa atacattttc tcgagcatcg tcacgaggta cgcgaactcg atgacgaaga
     2041 acccgtcatc cgcaagcaac tcccggacgt tccggagcac cgaacggata tcgtcgatgt
     2101 gagcgaagac attattcgcg ctgatcgcgt cggccttccc gtatcgggcg atgatctccg
     2161 cgaccgcttc ctcgccaaaa taacggttga gggtcgtaac gccgcgggtg ttcgcgtcgg
     2221 ccgcgagatt ctttgcgggc tccacgccga cgccgcgcag gccttctttt tcatacgagg
     2281 aaacgagcag cccgtcgttg ctcccgatat cgacggcgag ggcccccggc ttcgggccga
     2341 tccgttcccg gacgtcccgc gcgtattccg cgaaatgcgt ccggaacgtc tgtgtcgtcg
     2401 aactcacgta gagatattcg tcgaacatga gattcgcggg gaccgaatgg gtcaactgca
     2461 ccagatgaca ttccggacat ttgaccaggg cgagcgggca gcggaactcg ggctttcggg
     2521 cgttttcgat cccgggcaga ttattcgcga gcggctgcgc gccgaggtcc aggaacgcgg
     2581 tcccgagggg cgtttcgcag tagcggcagg tttttcgttt gtattcgtcg tagttcattc
     2641 gtcagttcct gacgcgggaa ccttctccgt tcaaccggaa cggaaagtct cccggggttg
     2701 tggttcaggc ggcgggcggc ggatagatcc ccgtaatgcg gccgtggcga atgacgatat
     2761 gaccgcccga aagaacatcg atcttcccct gaacgacacg gtcgatcacg tccgcgacaa
     2821 aatccgcgcc ctgttgccgc tcggcgtctt ctttcccgaa aagatcctga cgcatctgcg
     2881 tattcatccc tccgggacaa acagtgatgc actttaacgc ggctccttcg ttttccttgg
     2941 cgatcgcctg cgaaagagcg agaacgccgg acttggccgc ggaatacgca aaaagccgcg
     3001 gcaccgcgcg ggtgccggcc atcgaggaaa cattgaggac cagcggcttg ccgctggtcc
     3061 tcataaacgg aatcgcatac ttgcacatca ggaacgccga gacgaggttc tgccggagat
     3121 gcttctcgag ctcgtggacg ggggtatctt cgacccggac caggcggccg caataacccg
     3181 cgttgttcac gaggatatcg atccgtttat gcgtcttgtg gatcttctaa aggacggacg
     3241 aaacgctccg ttgggaagtg atatcacacc ggatcagtgt gaacgccggg gacggcagac
     3301 cggaccgtgc ctcatcccag tgtttcctgg tccggctgat cccataaacc gtgtccacct
     3361 cattgatgaa tcttttcgcc agctcaaagc ccaacccgcg actggctccg gtgattacaa
     3421 cgatacggct catgggaatt caggcctccc ggaatcttat cgggtcttca gttctttact
     3481 cgagtttggc acttttcata aaccaagaac agtgtttgga tcaacagaat ctctaaaatg
     3541 acgaatcatc tgacgaaagc ttctggattt gtttccgttc cacagggcaa tcaagttgta
     3601 cgcgattccg acaagagccc agtgtgcttg gactccatcc caggaatgca taaagacctc
     3661 gccaaacccg aggtgctgct tgatgtcttg gtggaaggtt tcgatccaga cccgctgaag
     3721 gtaaagagcg accatctgcg attcggtcat cttgagatcg ttggtgataa agaaccggat
     3781 accatctttc ctggatttgg aaataaacag aagcacagct ccgatcttgg gcaattggac
     3841 ttggagcttg gccacacgaa aacatttctt tctggaaaca cggattgttt tgtattttct
     3901 cggtcccttg gccagggagc tgagggagat cttctttccg ttgatttcca caagacgatt
     3961 tcgtttgacc gcggccacga atgtccaccc ggcttgaata ataagattga gaatcgcagc
     4021 gcaggaatac caggcatcca tcaaaaccgt gggcttgacc ggcaaagcct gaatggcttc
     4081 ggtaagaatc tcgacggcca gttggacttt gctcttgaaa gttccggctc tgcaagttcc
     4141 tttggggcga tatccccgga ttgcccagca aaag
//
LOCUS       DAOOBX010000036         4120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_24295_length_4120_cov_3.566052, whole genome shotgun sequence.
ACCESSION   DAOOBX010000036 DAOOBX010000000
VERSION     DAOOBX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24295_length_4120_cov_3.566052"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLY30_00980"
     CDS             <1..308
                     /locus_tag="PLY30_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64703.1"
                     /translation="GEIPAGYHVESGKNQGEFQERLWRDFWTYALDPAKRDRAGIKNA
                     QIEAPGSMFLPGTIYTLRIEHDGGIRIDAQPVPEILKGEKVASGTLTPDDGIRKVDG"
     gene            301..858
                     /locus_tag="PLY30_00985"
     CDS             301..858
                     /locus_tag="PLY30_00985"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HPW64704.1"
                     /translation="MGKIRRVSVFGSGTIREGSPVYRQAVKLGLALARAGFSVYHGGY
                     GGVMEAVAKGCRAAGGTNTGITVEARKMPGFKSYPGRALTRVNQWADAEIRMPSWEGR
                     LLKLIETGEAYVFLDGATGTLNELFLVWEMANRKLHTKPVLLLGKRLQKLVKFLKKDP
                     ALKIPESFYLVSSVDQTIKLLGAGR"
     gene            897..1970
                     /gene="queA"
                     /locus_tag="PLY30_00990"
     CDS             897..1970
                     /gene="queA"
                     /locus_tag="PLY30_00990"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPW64705.1"
                     /translation="MKLSDFDYPLPKEKIALFPTKERADAKLLCVDRGTGAIVHRIFR
                     DLPDLLRPTDLLVLNNAKVLPARLFGKKATGGKVEALLLKEVDANIWECLLKPGGRVK
                     EGQIVDFGVNGIELRAEVIDRPVPDSGQRFIRFPGEGIKEKIRILGHIPLPPYIDRPD
                     QTIDRSMYQTVFAENEGAVASPTAGLHFDEALLEGVRAKGVEVVPITLHVSYGTFQPI
                     AVENIGDHRMFEEVYEITEDAATRINRARREGRRIVACGTTCVRALESAVIHSTHRVG
                     GEETDPTYEVGSGVRKTSIFIHPPYEFKVVDALITNFHMPGSTLLVMIAAFLGYDKMM
                     AAYRIALEGDYRFASYGDAMFIG"
     gene            complement(2106..3428)
                     /locus_tag="PLY30_00995"
     CDS             complement(2106..3428)
                     /locus_tag="PLY30_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPW64706.1"
                     /translation="MISKVRKFLQHDIWRIRAREAKGFRGKGIRLLRIFLLSFRQFSG
                     DMCSFRASALTFYSLLSVVPVLAMSFGIAKGFGLDRLLREKLMEGIQGQQEILTRLIT
                     FAETLLENTKGGVIAGVGVAVLLWSVIGVLGNIETSFNNIWGIKKQRSLGRKFTDYLS
                     MMLIAPVLYVVAASATVFAAAKITAVARKIAILGAVAPLIAAGLKLLPFLVFAALLTY
                     LYIAMPNGKIHFRSALLGGIVAGALYQIVQWVYIRFQIGVSNAGAIYGSFAALPLFLI
                     WLQMSWRIVLYGAELAFSHQNELTFEFEHDCLNANYSVKKLLALRITHLCVSRFAAGK
                     PPLSAEEIAAQLEMPIRLARDLIFRLTAANILAIAQGENDRDRLYQPARDVGDMTVQF
                     VIDQMEKAGSSEVPSAKSPVLEKLRDSLAAFDQALRKLPENRLLKDLS"
     gene            3531..>4120
                     /locus_tag="PLY30_01000"
     CDS             3531..>4120
                     /locus_tag="PLY30_01000"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPW64707.1"
                     /translation="MKKFGFKLLGTDKKCNARVGHITTPHGKIETPVFMPVGTQGSVK
                     ALTPRDVKEAGSEIILSNAYHLYIRPGTEVIRKLGGLHKFMGWDRPILTDSGGYQVFS
                     LSKLRKISEDGVTFNSHFDGREIHLTPEKVVEIQETLGSDIAMIFDECPAASKDKHLI
                     EKAVNITLRWAKRAKKHHKRRSQALVMLFRAFRPAQG"
BASE COUNT          920 a         1129 c         1259 g          812 t
ORIGIN      
        1 caggcgagat tcccgccggc tatcacgtcg agtccgggaa aaaccagggc gaatttcagg
       61 aaaggctatg gcgggatttc tggacgtacg ccctcgatcc cgcaaagcgt gaccgggccg
      121 gtatcaagaa cgcgcagatc gaagcgcccg gttccatgtt tcttcccgga acgatctaca
      181 ccctcaggat cgagcacgac ggggggattc gcatcgacgc gcagcccgtt cccgagatcc
      241 tgaaagggga gaaagtcgcg tccgggactt tgacgcccga tgacgggatc cgtaaggtcg
      301 atgggtaaga tcaggagagt ttcggtcttt ggcagcggga cgatccggga aggttctccg
      361 gtctaccggc aggccgtaaa actcggtctg gcgctcgcgc gggcgggttt ttcagtctat
      421 catggcggtt acggcggggt gatggaagcg gtcgcgaagg ggtgccgggc tgcgggcggg
      481 acgaatacgg gtattacggt cgaggcccgg aaaatgccgg gtttcaaaag ttatccaggc
      541 cgggctttga cccgggtgaa tcagtgggcc gatgccgaga tcagaatgcc ttcctgggaa
      601 gggaggctcc tgaagttgat cgagacgggc gaggcctatg ttttcctcga cggggccacg
      661 gggacgctga atgagctttt tcttgtttgg gaaatggcga acaggaaact ccacacgaag
      721 cccgtccttc ttcttggcaa gcggctccag aaactcgtga aatttttaaa gaaagatccg
      781 gccctcaaga ttccggagag cttttacctg gtttcttcgg ttgaccaaac cattaaactg
      841 cttggcgcag ggcgctgaga ggggccttgc gccaaacgct tcgcgcgctc cacgccatga
      901 aactttccga tttcgactat ccgcttccca aagaaaaaat cgctctcttc ccgacgaaag
      961 agcgggccga cgcgaagctg ctttgcgtcg accgtgggac gggcgcgatc gtccaccgta
     1021 ttttccgtga tcttcccgat ctcctcagac cgaccgacct cctcgttctg aacaatgcca
     1081 aggtcttgcc cgcgcggctc ttcgggaaaa aagcgacggg cggaaaagtc gaagcgcttc
     1141 ttttaaaaga ggtcgatgcg aatatctggg aatgtcttct caaaccgggc gggcgggtga
     1201 aggaagggca gatcgtcgat ttcggcgtca acgggatcga actgcgggcg gaggtgattg
     1261 accggccggt tcccgattcg ggccagcggt tcatccggtt ccctggcgaa ggcatcaaag
     1321 agaagatcag gattctcggg catatcccgc ttccgcccta tatcgaccgt ccggaccaga
     1381 cgatcgaccg gagcatgtac caaacggtct tcgccgagaa cgaaggcgcg gtggcgtcac
     1441 cgaccgccgg gctccatttt gacgaagcgc tgctcgaagg ggttcgcgcg aaaggcgttg
     1501 aagtggtccc gatcacgctt catgtcagct acgggacctt ccagccgatc gcggtcgaga
     1561 atatcgggga tcaccggatg ttcgaagagg tctacgagat cacggaagat gccgccacaa
     1621 gaatcaaccg ggccagacgg gaaggtcggc ggatcgtggc ctgcggcacg acttgcgttc
     1681 gcgcgcttga atctgctgtc attcattcaa cacaccgcgt cggaggtgag gagaccgatc
     1741 caacctacga ggttggatcg ggtgtgcgga aaacctcgat cttcatccat ccgccgtatg
     1801 agtttaaagt agtggacgcg ttgatcacaa actttcatat gccgggaagt acactgcttg
     1861 tcatgatcgc ggcttttctc ggatatgaca agatgatggc cgcgtaccgg attgcgctgg
     1921 aaggggatta ccgtttcgcg agttacggtg acgccatgtt catcggttga acatggcgag
     1981 cggttccggt ttccctgaaa tgcaatcagg atcaggaagg cgatgccagg tttgtttttt
     2041 agcgcagggc ggatagcgtc gggaaaacca aaaatttcac aacctgtttt tgcccgcgct
     2101 ctacgctagg acaggtcctt gaggagcctg ttctcgggga gtttgcggag ggcctggtca
     2161 aaagccgcga gcgaatcccg cagtttttcg agcaccggcg atttcgctga aggaacttcg
     2221 ctcgacccgg ctttttccat ctggtcgatc acgaactgca ccgtcatgtc cccaacgtcg
     2281 cgggctggct gataaagacg gtcccggtcg ttctcgccct gggcgatggc caggatgttc
     2341 gctgccgtca gacggaagat cagatcccgc gcgaggcgga tcggcatctc aagctgggcc
     2401 gcgatctcct cggcggagag gggcggtttc ccggcggcga atcgtgacac gcatagatgg
     2461 gtgatccgga gagcgaggag ttttttaacc gaataattgg cgttaaggca atcgtgttcg
     2521 aattcgaatg tgagttcgtt ctgatgcgaa aaggcgagct ccgcgccgta aagcacgatg
     2581 cgccagctca tctgcagcca gatcagaaaa agcggaaggg ccgcgaaact tccgtagatc
     2641 gcgcccgcgt ttgaaacgcc gatctgaaac cggatataaa cccattgaac gatctggtac
     2701 agcgcgcccg cgacgatacc gccaagcaac gcggacctga aatggatttt tccgttcggc
     2761 atcgcgatgt aaaggtaggt caggagagcc gcgaatacca ggaaggggag gagtttgagc
     2821 cccgcggcga tgagcggcgc cacggcgccc aggatcgcga tcttccgggc gaccgccgtg
     2881 atcttggcgg ccgcgaagac ggtcgcgctg gccgcgacga cgtagaggac cggggcgatc
     2941 agcatcatcg agaggtaatc cgtgaacttg cgccccaggc tccgctgttt cttgatcccc
     3001 cagatgttgt tgaacgacgt ttcgatgttc ccgaggaccc cgattaccga ccagagcagg
     3061 actgcgacgc cgacccccgc gatgacaccc cccttcgtgt tctcaagcag ggtctccgcg
     3121 aacgtgatca gccgcgtcag gatttcctgc tgcccctgta tgccctccat gagcttttca
     3181 cgcaaaagcc tgtcgaggcc gaaacccttt gcgatcccga atgacatcgc gagcacggga
     3241 acgaccgaaa gaagagaata gaaggtgaga gccgacgccc tgaacgaaca catgtcgcct
     3301 gaaaactggc ggaaggaaag caggaaaatc cgaaggaggc gaatgccttt cccgcggaag
     3361 ccctttgctt cccgcgcccg gatgcgccag atgtcgtgct gaaggaattt ccgaactttc
     3421 gaaatcatga cgggaattgt atcacaccgt gacccgggag cgtccagttt ttatgttccg
     3481 aagaaagccc aaatacatat aatacgccgc tgacctggga gcggctttca atgaagaagt
     3541 tcggatttaa actgctggga acggacaaaa agtgcaacgc gagggtcggg catatcacga
     3601 cgccgcacgg gaagatcgag acgcccgttt ttatgccggt cggcacgcag gggagtgtga
     3661 aagcgcttac gccgcgcgat gtcaaggaag cgggaagcga gatcatcctt tcgaacgcct
     3721 accatctcta catccgtccg ggaacggagg tcatcaggaa actcggcggg ctccacaagt
     3781 tcatgggctg ggaccggccg atcctcaccg acagcggtgg ctaccaggtg ttttccctga
     3841 gtaaactccg gaagatttcg gaagatggcg tcacgttcaa ttcccacttc gacggccgcg
     3901 agatccatct cacaccggaa aaagtggtcg aaatccagga aaccctcgga agcgacatcg
     3961 cgatgatctt cgatgaatgc ccggccgcgt ccaaggataa acatctgatc gaaaaggccg
     4021 ttaatataac cctgcgctgg gcgaaacgcg cgaaaaagca tcacaagcgc cggtcccagg
     4081 cgcttgtgat gctttttcgc gcgtttcgcc cagcgcaggg
//
LOCUS       DAOOBX010000037         4084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_24551_length_4084_cov_2.999255, whole genome shotgun sequence.
ACCESSION   DAOOBX010000037 DAOOBX010000000
VERSION     DAOOBX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24551_length_4084_cov_2.999255"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="PLY30_01005"
     CDS             complement(<1..1683)
                     /locus_tag="PLY30_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW64708.1"
                     /translation="MGLPSVSLKRPVFTAIIFAAVLVLGMISLMRLKVELYQGQSQGI
                     VSIVVRARGGLPSQDVEKMITKPIEEAVGTVSKMKNLYSSSREAESRVTMEFEPGTNM
                     NFAALEIREKFARVRPLLPKEIEKPVIANYDDAQAAILVFAVTSDSMTPEQIREMVDI
                     KLKPILSRVDGVASVEVYGGRERKILVELDRDKLVAYNISVERVMDVLGQSNINLLAG
                     NVETGKYEYAIRSMGSFLTVDEIGELGVKATRQGSIISLKEIATVKDSYLEATDQARL
                     NLEQNVTVYVKKASLANTLDVADDLTRVLDAFEADHKDSIKIVFVNDRADAIKRAIDD
                     VKLALLIGLVLTMGVIYFYIMRWRLSAIILVVIPASLMATFICMAAAGISINVMTLSG
                     LSLAVGLLVDTAVVIMENIFSKREGGASAAHAIRDGSEEVWLPLLASMTTTLVVFLPI
                     VFIDKTIQLTYGGFAFTICVALIASFFSALMLIPMLIKQFAVAELDARTQTLSDRFIH
                     KMKDLYQGALHGVFRARWLVVGIMVVACCLGIFGIAKTPNDWPSSFEENEFAV"
     gene            complement(1687..>4084)
                     /locus_tag="PLY30_01010"
     CDS             complement(1687..>4084)
                     /locus_tag="PLY30_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW64709.1"
                     /translation="VKDIGSVTDGLADKTSISRFNGADNITLSIQKQSNANTIQTVDR
                     LRKELDFLEEDLVSRGVKVDIIYDHSVFIRNTIKNLAHEATQGGILALITLWAYLKSF
                     LMSVLVVVSLPLTILGVFLLMALSNVTVNIMSLGGLAIAIGMITDTSIVVLENIFRKR
                     QAGMNAEQGAIEGAAEVVWPVISSNLTTIAVFFPLIVFVPGVAGQIFKDLAWSIIFSQ
                     IISTFIPLTLVALLSTYVMIKQQDYQPIDLLAPLERSVRSQPTHGAQNGIMGSVLIIV
                     FVILASTFFVVPTMDREVLPKTDQGQFYVKVSMPLGTRLGVTDRVCMRVEEVLREEED
                     VKDISVAIGSEKTSRGEIKIETLRPSQGQILVTLNQNRKRTSSAVVASLKKKTDALGL
                     ERESAKVEYVLQESEFAVGTQAVKPILIEVKGYDFRIMDEFVSRLKSKLSGIPGIIHI
                     EDDIGEKSPETKLDINKKRAALYGISALDISLTAKAAIEGVVATQFREGGREFDVRVR
                     LSEKDRSDIEGLGDLLLYSKILDVHVPMKELVVIERGQGPSEIKRADQERTVTISADI
                     DKSRQDKDVLADLQRFLAGLKVPDDIPEDFQVKLSGKAKEIKENFGGVTFAFMLAVML
                     VYMIMAAQFESFLQPLIIMVTVPLSFGGAILALKLTGNTLNVISLLGLVLLGGVVVAN
                     GIVLMEYINQLRAEGKEMVDAVWEAVKTRTRPILMSATTTILAMLPVALALSGEGADL
                     LSPLAVTAMGGLFSSTLLTLVVLPCLYILVNRGFERFLGYEEELSPAAGAEEESLPGD
                     "
BASE COUNT          909 a         1202 c         1135 g          838 t
ORIGIN      
        1 aaccgcgaac tcgttttcct cgaaggaaga cggccagtcg ttcggcgtct tggcaatccc
       61 gaaaatcccg aggcaacagg cgaccaccat aatccccacc acaagccagc gggcgcggaa
      121 gaccccgtgg agcgctcctt gataaagatc tttcatctta tggatgaagc gatccgaaag
      181 agtctgggta cgggcatcga gctcggccac agcgaactgc tttatcagca tcgggatcag
      241 catcaaggcc gagaaaaaag aggcgatcag cgccacgcag attgtgaacg caaaacctcc
      301 gtaggtcagc tgaatggtct tgtcgataaa aacgatcggc agaaacacga ccagcgttgt
      361 cgtcatggac gccaaaagcg ggagccagac ctcttcgctt ccgtcacgga tcgcatgcgc
      421 cgcggaggcc ccgccttccc ttttggaaaa tatgttctcc ataatgacca ccgcggtatc
      481 caccaggaga ccgaccgcca gtgaaagacc ggaaagcgtc atcacgttga tactgatgcc
      541 cgccgccgcc atgcagatga atgtggccat gagagaggca gggatcacaa caaggatgat
      601 cgcggaaaga cgccagcgca tgatgtaaaa atagatcacc cccatggtca ggaccaaccc
      661 aatgagcagt gccagtttga catcatcgat cgcccgtttg atcgcatccg cccggtcgtt
      721 gacgaagacg attttgatcg aatccttgtg atcggcctcg aaagcgtcca gaacccgcgt
      781 caggtcgtcg gccacatcca gcgtgttcgc gagactcgcc tttttgacgt agaccgtaac
      841 gttctgttcc aggttgaggc gggcctggtc cgtcgcctcg aggtaggaat ccttgaccgt
      901 cgcgatctct ttcagggaaa tgatcgatcc ctgtcgcgtc gctttcacgc cgagctcccc
      961 gatctcgtcc acggtcagga acgaccccat gctccggatc gcgtactcgt attttcccgt
     1021 ctcgacgttc ccggcgagga gattgatgtt gctctgaccg agtacgtcca tgacgcgctc
     1081 caccgaaatg ttataggcca ccagtttgtc ccggtcgagt tcgacaagga tcttccgttc
     1141 gcgtcctccg tagacctcga cgctcgcgac gccgtccacg cggctcagga tcggcttcag
     1201 cttgatatcc accatctcac ggatctgttc gggcgtcatg gaatccgagg tgactgcgaa
     1261 cacgagaatg gccgcctgcg cgtcgtcata gttggcaatg accggctttt cgatctcctt
     1321 cgggagaagt ggccgcaccc gcgcgaactt ctcgcggatt tcgagcgccg cgaagttcat
     1381 gttggttccc ggttcgaact ccatcgtcac gcgggattcg gcctcgcggg aactcgaata
     1441 gaggttcttc attttactga cggtcccgac ggcttcctcg atgggcttcg tgatcatttt
     1501 ctcaacgtcc tgggaaggca gaccgccgcg ggcgcggacc acgatgctga cgatcccctg
     1561 gctctggccc tgataaagct ccaccttgag acgcatcagg gagatcatcc cgaggacaag
     1621 gacagcggca aagatgatgg ccgtaaaaac cggccgcttg agggagacgc tcgggaggcc
     1681 catgcgctag tccccgggaa gggactcttc ttcggctccg gccgcagggg acagctcctc
     1741 ctcatagccg aggaaccttt caaagcccct gtttacgagg atataaaggc acggcaggac
     1801 gaccagagtg aggagcgtgg aagaaaaaag gccgcccatg gcggtcacag ccagcggcga
     1861 cagaaggtcg gcaccttcac ccgacagagc gagcgcgacc ggcaacatgg caaggatggt
     1921 cgtcgttgcc gacatcagaa tgggacgcgt acgggtcttc acggcctccc acacggcatc
     1981 gaccatttcc ttcccttcgg cacgtagctg attgatgtat tccatgagca cgataccgtt
     2041 ggccacgacg accccgccaa gcaacacgag cccgagaaga gaaatgacgt tcagcgtgtt
     2101 ccccgtcagc ttgagagcca gaatcgcgcc gccgaaagaa agaggcacgg tgaccatgat
     2161 gatgagcggt tggaggaacg attcgaactg ggccgccatg atcatgtaga cgagcatgac
     2221 cgcgagcatg aacgcgaacg tcactccgcc gaagttctcc ttgatctcct tcgccttgcc
     2281 ggacagcttg acctggaaat cctcggggat atcgtccggc accttgagac cggcgaggaa
     2341 ccgctggaga tccgccagga cgtccttgtc ctggcgcgat ttgtcgatat cggctgaaat
     2401 ggtcacggtg cgctcctggt cggcacgctt gatctcactc ggcccctggc cacgctcgat
     2461 cacgacgagc tctttcatcg ggacatggac atcgagaatc ttggaataaa gaagcaggtc
     2521 cccgagacct tcgatatcgg agcggtcctt ctcactcaac cggacgcgga catcgaactc
     2581 ccgccctccc tcgcgaaact gggtggccac cacgccctcg atcgcggcct tcgccgtcag
     2641 ggagatatca agggcagaga taccgtaaag cgccgcgcgt tttttattga tatccagctt
     2701 ggtctcgggg gatttttcgc cgatgtcatc ctcgatatgg atgatccccg gaatacccga
     2761 cagctttgat ttcagccgcg aaacaaattc atccatgatc ctgaaatcat accccttgac
     2821 ctcgatcagg atgggcttga ccgcctgcgt gccaaccgcg aactcgcttt cctggaggac
     2881 atattcgacc ttggcgctct ctctttcgag accaagggca tccgtttttt tcttcaggga
     2941 tgcgaccacg gcggatgatg tccttttccg gttctgattg agggtcacaa gaatctgccc
     3001 ctgggacggg cgaagcgttt cgatcttgat ctcgcctcgg ctcgtttttt cgctaccgat
     3061 cgcaaccgaa atgtccttca cgtcctcttc ttcacggagg acttcctcga cccgcatgca
     3121 aaccctgtcg gtgactccga gacgtgtccc cagcggcatg ctcaccttaa catagaattg
     3181 tccctggtcg gttttcggga gtacttcgcg gtccatcgtg ggaacaacaa agaatgtcga
     3241 cgcaaggatc acaaaaacga ttatcaggac gctacccatg atcccgttct gggccccgtg
     3301 agtcggctgg cttcgaacgc tccgttcaag cggggccaga agatcgatcg gctgatagtc
     3361 ctgctgtttg atcataacgt aagtggaaag cagcgccaca agggtaagcg ggatgaacgt
     3421 tgagatgatc tgcgaaaaaa tgatgctcca ggcaaggtcc ttgaagatct gacctgctac
     3481 gccgggaacg aaaacgatca gggggaaaaa gacagcgatc gtcgtgaggt tcgaggagat
     3541 cacgggccag acgacctccg cggcgccttc aatcgcgccc tgttccgcgt tcatgccggc
     3601 ctgacgtttt cggaaaatgt tctccaggac aacgatactc gtgtccgtga tcatgccgat
     3661 cgcgatcgcg agcccgccaa gggacatgat gttgaccgtc acattcgaca gcgccatcag
     3721 caaaaagacc ccgagaatgg tcagcggcag ggatacaacg accagcaccg acatcaggaa
     3781 gcttttgaga tacgcccaca gggtgatcaa cgcgagaata ccgccctggg tggcctcgtg
     3841 ggcgaggttc ttgatcgtat tgcggataaa gaccgaatgg tcgtagataa tgtccacctt
     3901 gacgccgcgg ctcacaagat cctcttcgag gaaatcgagc tccttgcgaa ggcgatccac
     3961 ggtctggatt gtgttcgcgt ttgactgttt ctggatggag agcgtgatgt tatccgcccc
     4021 gttgaaccgg gaaatactcg tcttgtccgc gagcccgtca gtcaccgatc cgatatcctt
     4081 gaca
//
LOCUS       DAOOBX010000038         4054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_24805_length_4054_cov_3.988997, whole genome shotgun sequence.
ACCESSION   DAOOBX010000038 DAOOBX010000000
VERSION     DAOOBX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24805_length_4054_cov_3.988997"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLY30_01015"
     CDS             <1..234
                     /locus_tag="PLY30_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64710.1"
                     /translation="ILPLFNIPLIMRVVKRKSADDISLVWLFGVWICILLMSPAAFVS
                     NDTVFRLFGYANLLFFTGVVFVTVKYHRKFKRN"
     gene            320..625
                     /locus_tag="PLY30_01020"
     CDS             320..625
                     /locus_tag="PLY30_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPW64711.1"
                     /translation="MLEGLAKKETIKKKKRVGDKWSLYILKCGDGSLYTGITNDIERR
                     FRMHQNGKAARYTRTRRPVELVYQETCGTRSRALIRECEIKEWPRKKKEKLFSGTRD"
     gene            complement(716..1315)
                     /locus_tag="PLY30_01025"
     CDS             complement(716..1315)
                     /locus_tag="PLY30_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64712.1"
                     /translation="MSNDELRGKVVEVARKHKASWIELGQYLFSISKDRIFREWGYNT
                     FEAYCVRELKIREATASKLLKSYSFLEREEPRMVKPEYTTEEEPRKIPDYEAVNLLRL
                     AKQNKNIPVQEFAELRHEVLNEAREFRDVRAKVKAIVAERKPKDTPEAREAKRSSAIR
                     RLIGFLNGAKKQLKDEDLVPDHLLRQIEALAAKLEDQLH"
     gene            complement(1413..2300)
                     /locus_tag="PLY30_01030"
     CDS             complement(1413..2300)
                     /locus_tag="PLY30_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW64713.1"
                     /translation="MKTSPQLIRVGHSPDPDDAFMFYALAHEKIDMRGFRVEHVIEDI
                     ESLNQRALKGELEVTAVSCHAFAYLSERYAVMRSGASVGDDYGPILVKKPGSGGRGSG
                     PDFENKKIAVPGKLTTASLVLQLYAKTGSNSPHSALRMPYKAVFTPFDKIFDAVKNGE
                     ADYGLIIHEGQITYEKHGFELAADLGKWWLAHTGLPLPLGIDIIRKDLGPETMRRFAE
                     LFRESIQYALNHRKEALKYASRFGRGLADDLNDRFVGMYVNDYTVELGANGEAGFRRL
                     MDEAYAAKIIPHQVKPEFI"
     gene            complement(2409..>4054)
                     /locus_tag="PLY30_01035"
     CDS             complement(2409..>4054)
                     /locus_tag="PLY30_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64714.1"
                     /translation="SHPDEGVRKTAKLFLGHELVSGGRKGFEGLFRASRFLESLKPPV
                     TVRAEARTSVKKAKGPSSAEKSRAALVTALEQKPDVELIRRYGAMNKRAIPEVVQDEI
                     AAIVDRFETQFTLSKDDRAFLIVTIGEYAAAVEWAKKDEAAGETATLEKLYADSLVIK
                     RGGREYPRDTEIGITVRGTKRVVRVSGRFIKENQDGIRTILEALVPPPAKTVQGMPPV
                     ISLKDLFGHNTERPLRPQEEIALALGVKLGLWKLEREGTGITVIPSVKTENESAAEAP
                     LDGSAAPRAETRSTETRPQKIAGELRGLVTASGPVLTYKEFLLDHERLSGASERAMRA
                     VTEKIMDGIVPLLAYSYEIKNIILENLAPVARVTAPVTVASWPEKFQSEFTVRMLRRS
                     LGVKTLQANDALVLDPETAFKRGLVYVIRNVFGNIRTVVLTEDPADLGFLEKINARLR
                     QANRPPILPAADLDEATRKLLDADRETGARGTSLNLKAMIFAGANNPQAALLAEQLKD
                     NVVTIDQKMFRNFLNLAGLGVSQMVEQMQAEYLATARSA"
BASE COUNT          813 a         1193 c         1115 g          933 t
ORIGIN      
        1 atcctccccc tgttcaacat cccgcttatc atgagggtcg tgaagcgaaa gtccgccgac
       61 gacatcagcc tggtctggct cttcggcgtc tggatctgta tccttctcat gtcacccgcg
      121 gccttcgtat cgaacgatac tgtgttccgg ctcttcggct acgcgaacct tctttttttc
      181 acgggcgtcg tttttgtgac tgtgaaatat caccgtaaat tcaaaagaaa ctaggcgccg
      241 ggacccggac ccgcagagtc catggaaaca aacgggttcc tgatcacgga tttttaccat
      301 gcatcccaaa tacaaacgca tgctggaagg cctcgcaaaa aaagagacga tcaaaaagaa
      361 aaagcgcgtc ggggacaaat ggtcgcttta tatcctgaag tgcggggacg ggtctctcta
      421 cacggggatc acgaatgata tcgaacggcg cttccggatg caccagaacg ggaaggcggc
      481 ccgctacaca cggacgcggc gaccggtcga actcgtttat caggaaacct gcggcacgcg
      541 ttcccgggca ctgatccggg agtgcgagat caaggaatgg ccgcgaaaaa agaaagagaa
      601 actgttttca ggaactcggg actagctcgc cgcaaggcat cggatgaatc cgcccgggag
      661 gtttggcgga tgcccggaag ctcgtaaaat ccggggacac aaacaaattc cggtcttagt
      721 ggagttgatc ttcgagcttt gctgccaggg cctcgatctg cctgaggaga tgatcgggaa
      781 cgaggtcttc gtctttcagc tgttttttag cgccgttcag aaacccgatc aaacgccgga
      841 tcgcggaact acgctttgct tccctggcct cgggcgtgtc cttcggcttc cgttccgcga
      901 caatcgcttt taccttcgcg cggacatccc tgaattccct ggcttcgttg agaacctcat
      961 ggcggagttc cgcaaactcc tggaccggta tgttcttgtt ctgtttcgcg agacgcagca
     1021 ggttcacggc ctcgtaatcc gggatcttcc ggggctcctc ttcggtcgtg tattccggtt
     1081 tcaccatgcg cggctcctcc ctttcgagaa acgagtaaga tttgaggagt tttgaagccg
     1141 tcgcctcacg gatcttgagc tccctgacgc agtacgcttc gaaggtgttg tacccccatt
     1201 ccctgaaaat cctgtccttt gagatggaga aaaggtactg tccgagctcg atccagctcg
     1261 ccttgtgttt ccgtgccact tccaccactt taccgcgcaa ttcgtcgttg ctcataaatt
     1321 ccttctatat ggagtgagaa gaggtgcggg gcacggagcc cgctttgacg gattttattc
     1381 cgcgcctgaa actccgcatt ccgtaccggt atttaaatga attccggttt cacttgatga
     1441 gggatgatct tcgcggcgta cgcttcgtcc atcaacctgc gaaaccccgc ttcgccgttc
     1501 gcgccgagtt caaccgtgta atcgttcaca tacatcccga caaaccggtc gtttaagtcg
     1561 tcggcaagac cccggccgaa tcggctcgcg tattttaatg cttctttccg gtgattcaag
     1621 gcatattgaa tgctttccct gaagagctcc gcgaaccgcc gcatcgtctc cggcccgagg
     1681 tcctttcgga tgatgtcgat tccgagggga agcggcagcc ccgtgtgagc cagccaccat
     1741 tttccgagat cggcggcaag ctcgaagccg tgtttttcat aggtgatctg gccctcgtgg
     1801 atgatgagac cgtagtccgc ctcgccgttt tttaccgcat caaaaatctt gtcaaatggc
     1861 gtaaaaaccg ctttgtacgg catgcggagt gcggagtgcg gggaattgct tccggttttt
     1921 gcgtacagct ggaggaccag actggctgtt gtcagttttc ccggaaccgc gattttttta
     1981 ttttcaaaat cggggcccga accccggccc ccggacccgg gctttttaac aaggatcggc
     2041 ccgtaatcgt caccgacgct cgcacccgag cgcatgacgg catatctttc ggaaagatac
     2101 gcgaaggcgt gacaggagac ggccgtgact tcgagctccc ctttcagcgc ccgctgattg
     2161 agggactcta tgtcttcgat cacgtgctcc acgcggaacc cgcgcatatc gatcttctcg
     2221 tgcgcgagcg cgtagaacat gaacgcgtcg tcgggatcgg ggctgtggcc gacccggatc
     2281 agttgcgggg aagttttcat aggagcgata ttatatgcca cacaccgcat aaatcgccac
     2341 aagtactctg ccgcaccccg catgaaaacc tttcagaaag ggcggcgatc ttgcggccct
     2401 gtgacagctt acgcgcttct cgccgtcgcg aggtactcgg cctgcatctg ttcgaccatc
     2461 tgactcacgc cgagaccggc caggttgagg aagttccgga acatcttctg gtcgatcgtg
     2521 acaacattgt ccttcaactg ctcggccaga agcgccgcct gcggattgtt cgctcccgcg
     2581 aaaatcatgg ccttcagatt cagggaagtg ccgcgggccc cggtttcccg atcggcgtcc
     2641 aggagttttc tcgtcgcttc atccagatca gcggccggca ggatgggagg ccggttggcc
     2701 tggcggagcc gggcgttgat cttttcaagg aaaccaaggt ccgcaggatc ttccgtcagg
     2761 acaaccgtgc ggatattgcc aaacacattg cggatcacat aaacaagacc gcgcttgaaa
     2821 gcggtttcgg ggtcaagcac aagcgcgtcg ttcgcctgga gcgttttgac cccaagcgac
     2881 cgccgaagca tccggacggt gaattcgctc tggaatttct ccggccagct cgccaccgtg
     2941 acgggcgcgg tcaccctggc gaccggggcc agattttcaa ggatgatgtt tttgatttca
     3001 taggaatacg cgagaagcgg tacgatcccg tccatgatct tttccgtcac ggcgcgcatc
     3061 gcgcgttcgg acgcaccact caggcgttca tgatccagga gaaactcctt ataggtcaac
     3121 accgggccgg aggccgtgac aagacctctc aattcgcccg cgatcttctg aggccgtgtt
     3181 tctgtcgaac gcgtttccgc gcggggggca gcagagccgt ccaaaggcgc ctcagccgcg
     3241 gactcgtttt ccgttttaac ggacgggatg acggtaatgc cggtcccttc gcgctcgagc
     3301 ttccagagac cgagcttcac acccagggca agcgcgatct cttcctgcgg ccgcagcggt
     3361 ctttcggtgt tgtgcccgaa aaggtccttc agggaaatga ccggcggcat tccttgaacc
     3421 gttttggccg gcggcggcac cagggcctcc aggatcgtcc ggatcccgtc ctggttttct
     3481 ttaataaacc ttccgctaac acggacaacg cgtttcgttc cgcggaccgt tatcccgatc
     3541 tccgtatctc tcggatattc cctgccgcca cgcttgatca cgagagaatc cgcatagagc
     3601 ttttcaaggg tcgccgtttc cccggccgct tcgtcttttt tagcccactc cacggcagcg
     3661 gcgtattcgc cgatcgtcac gatcagaaat gcccggtcgt ccttcgaaag cgtgaactgt
     3721 gtttcaaaac ggtcaacaat cgccgcaatc tcatcctgta ccacctcagg gatcgcccgt
     3781 ttattcatcg cgccgtagcg gcggataagt tcgacatccg gtttctgttc gagggcagtc
     3841 acgagggcgg cccggctctt ttcggcggag gacggtcctt tcgccttttt cacggaagtc
     3901 ctggcctcgg cgcggaccgt caccgggggt ttcagacttt caagaaagcg gctcgcgcga
     3961 aacagccctt caaaaccttt gcggccaccc gaaaccagtt catgcccgag gaacaatttt
     4021 gcggtcttgc gaacgccttc gtcaggatgg gagg
//
LOCUS       DAOOBX010000039         4029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_24990_length_4029_cov_3.182436, whole genome shotgun sequence.
ACCESSION   DAOOBX010000039 DAOOBX010000000
VERSION     DAOOBX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24990_length_4029_cov_3.182436"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLY30_01040"
     CDS             complement(<1..263)
                     /locus_tag="PLY30_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64715.1"
                     /translation="MMPSFSYLVKDKSGKDVTGVEDAPDTGELVRKLKAQGFMVIRIE
                     EVKRTQPIFKVTKGAAAASPVKKQKPGKSGGVKIDDMVVFARQ"
     gene            complement(265..1965)
                     /locus_tag="PLY30_01045"
     CDS             complement(265..1965)
                     /locus_tag="PLY30_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPW64716.1"
                     /translation="MKIEKTGWVEWLKEMGVAEDQIARLEQTAERQDISMVDAIIREK
                     AVTEDVLIDFMSEKLSIPPLNIQAVQVDQDLVRMFTKKFIEQHRVIPVARIGKLVTVA
                     TGDPFKLRVFDDIKRTTGCDVVLTLVLPSKLAEVIDNFFSETKNFDEFIGGEDADNVE
                     VIQENEEPDSRDTAQVGVEDAPVIKMVNFILEKAIKQRASDIHFEPYEKEFRIRYRID
                     GALRESFSHALDFYPALVARTKIISKLDITEKRIPQDGRFRLFLKDRQIDFRVSVLPT
                     YFGEKMVLRVLDRSGIRSGLDKLGFTEKPTARLADAIKRPYGMILVTGPTGSGKSTTL
                     YSILNSLNTPEKNLMTVEDPVEYQVEGITQTQVIPEIGLTFASGLRALLRQSPDIVLV
                     GEIRDGETADIAIKAALTGHLVFSTLHTNSAAGAMTRLMDMGIEPFLIASSVVCVAAQ
                     RLLRGICPYCKAPATISPDVLRRCKIPEAELRSITPFSGKGCAKCNNTGYFGRLGTAE
                     VLTIDPEIRNLVIQKKSSNVIHEVAVQKGMETLFENALGLFKAGRTTLEEVLRVTSAG
                     "
     gene            complement(1962..3758)
                     /locus_tag="PLY30_01050"
     CDS             complement(1962..3758)
                     /locus_tag="PLY30_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPW64717.1"
                     /translation="MTEKPDFGIDIKALLAREKIATAEDIEWAEKECAKNPRKQVIDI
                     LLDAGKCDEKKLVSFLSALYGYRPVSLNMLVIHEEVLALIPQKSAEEHRCLPVSLLDK
                     TLTVAFANPTNLKAIDDIQVVSGKRIRATVAECSVIKELIAKCYQGGSQAGAVPADVS
                     KLPGVATSEPETDELIALARQAGEESPGVKQEQTADLMQLASQAPVVKLVNMMLLEGV
                     RRRASDIFIEPWENHVRVRMRVDGILEEIVRPQRGYASAIVSRIKIMSQLNIAEHRVP
                     QDGRFKVRTQGREVDMRVSILPSSFGEKVCLRILDTGSQAHDISKLGFTEKEQQIIRD
                     SAKKPHGMILVTGPTGSGKTTTLYSILKYLDSPQVNITTVEDPVEYQIPGINQVNVRE
                     AIGLTFPAALRSILRQDPDICLIGEIRDAETLDIAIKAALTGHLVLSTLHTNDAVSSV
                     TRMVNLGLEPFLIASTVLMISAQRLVRKLCPRCRYAYDLEPEMYPLLNLDPARKVQFW
                     RAKGCSQCRQMGYQGRSVITEILEMSPGILDLIMQGASADDIKQRARELGMNTLRESA
                     VRKAFAGETSIEEVFRVSTEDQALKKGTGVRP"
     gene            complement(3775..>4029)
                     /locus_tag="PLY30_01055"
     CDS             complement(3775..>4029)
                     /locus_tag="PLY30_01055"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64718.1"
                     /translation="PYIPLARYALNPDMASLLEADFCGMHHVVAFDGDARSVFLAIAN
                     PLADEAVSKVRELTGREPKVFISRISEILNAIFYLYHEKK"
BASE COUNT          771 a         1215 c         1153 g          890 t
ORIGIN      
        1 atctgacgcg caaagaccac catatcgtca atcttgaccc cgcccgactt gcccggcttc
       61 tgttttttaa caggcgacgc ggcggccgca cccttcgtga ccttgaagat cggctgggtc
      121 ctcttgacct cctcgatccg gatcaccatg aaaccctgag ccttgagctt gcggacgagt
      181 tccccggtat ccggggcgtc ctccaccccg gtcacgtcct taccactttt atccttcacc
      241 agatacgaaa atgacggcat catttcaccc cgcgcttgtg actcgcagga cctcttcaag
      301 cgtggtcctc cccgccttga acagtcccaa tgcgttctca aaaagcgttt ccattccctt
      361 ttgaacggca acttcgtgaa taacattact ggacttcttc tggatgacca ggttccggat
      421 ctcgggatcg atcgtcagga cctcggcggt cccgaggcgg ccaaaataac ctgtgttgtt
      481 gcacttggcg caccccttgc cgctgaacgg cgtgatcgac ctgagttcgg cctccgggat
      541 cttgcaacgt ctcagcacat cgggcgagat cgtcgcgggc gccttgcaat acgggcagat
      601 cccgcgaaga agccgctgcg ccgcgacaca gactacggaa gacgcgatca ggaacggttc
      661 gatccccata tccatcagac gcgtcatggc accggcggcg ctgttggtgt ggagtgtgct
      721 gaagaccagg tggccggtca gggccgcctt aatcgcgatg tcggccgttt cgccgtcgcg
      781 aatctctccc accagtacaa tatcggggct ctggcggagg agggcccgca ggccgctcgc
      841 gaacgtcagc ccgatctccg gaatcacctg tgtctgcgta atcccttcca cctggtactc
      901 gaccgggtct tcgaccgtca tcaggttctt ctcgggcgtg ttcagggaat tcaggatcga
      961 ataaagcgtc gtggacttcc cgcttcccgt cggtcccgtc acgaggatca tgccgtacgg
     1021 ccgcttgatc gcgtcggcga gccgcgcggt cggtttttcc gtgaaaccga gtttgtcgag
     1081 cccggaccgg atgccgctgc gatcaagaac acgaagcacc atcttctccc cgaaataggt
     1141 cggcaggaca gacacccgga aatcgatctg ccggtccttc aggaaaaggc ggaaccggcc
     1201 gtcctgcggg atgcgctttt cagtgatatc cagtttggag atgatcttgg tcctcgcgac
     1261 aagcgccgga taaaaatcca gggcatgtga aaaggattcg cggagcgctc catcgatacg
     1321 gtaccggatc cggaattcct tctcgtaggg ttcgaagtgg atgtccgagg cgcgctgttt
     1381 gatggccttc tcgaggatga agttcaccat cttgatgacc ggcgcgtcct cgacgccaac
     1441 ctgcgccgtg tcgcgggaat ccggttcctc attctcctga atgacctcga cgttatcggc
     1501 gtcctcgcca ccgataaact cgtcgaaatt cttcgtctcg gaaaagaaat tgtcgatgac
     1561 ttcggcgagt ttcgagggaa ggaccagcgt cagcaccaca tcgcagcccg tcgtccgttt
     1621 gatatcatcg aagaccctca gcttgaaggg atcccccgtc gcgaccgtga ccagtttccc
     1681 gattctggcg acagggatca cgcggtgctg ttcgatgaac tttttcgtga acatacggac
     1741 cagatcctgg tccacctgga ccgcctggat gttgagcggc ggaatggaaa gcttttcgct
     1801 catgaaatcg atgagcacat cttccgtcac cgctttttca cggatgatcg cgtccaccat
     1861 gctgatatcc tgacgctcgg cagtctgttc gaggcgggca atctggtcct cggccactcc
     1921 catctctttc agccattcca cccagccggt cttttcgatc ttcatgggcg gacccccgtt
     1981 ccttttttga gggcctgatc ctcggtcgac acgcggaaaa cctcttcgat cgaggtctcg
     2041 cccgcgaagg cctttctcac ggccgattca cggagcgtgt tcatcccaag ctcccgggcc
     2101 ctttgtttga tgtcgtccgc ggacgcgccc tgcatgatga ggtcgagaat cccgggagac
     2161 atctcgagaa tctccgtaat cacgctgcgt ccctgatacc ccatttgccg gcactggctg
     2221 cagcctttgg ctctccagaa ctggaccttg cgggcgggat ccaggttcag aagcggatac
     2281 atctccggtt ccagatcgta ggcgtagcgg caacgcgggc aaagtttcct gacgagacgc
     2341 tgggccgaga tcatgagcac cgtggacgcg atcaggaacg gctcaagccc gaggttgacc
     2401 atacgcgtga cggagctcac cgcgtcgttc gtgtggagcg tgctgagcac gaggtgcccc
     2461 gtcagggccg ccttaatggc gatatcgagc gtttccgcgt cgcgtatctc gccgatcagg
     2521 cagatgtccg ggtcctgtcg caggattgat cgcagcgccg ccggaaaggt caggccgatc
     2581 gcctcgcgga cgttcacctg gttgatgccg ggaatctggt attccaccgg atcctcgacg
     2641 gtcgtgatgt tgacctgggg cgagtcgaga tatttcagga tggaatagag tgtggtggtc
     2701 ttgccgcttc ccgtgggacc ggtgacgagg atcattccgt gcggcttttt cgccgaatcg
     2761 cggatgatct gctgttcctt ttcggtgaac ccaagctttg aaatatcatg cgcctgagaa
     2821 cccgtgtcca ggatgcggag acagaccttt tcgccaaaac tggatggaag gatggagacg
     2881 cgcatgtcga cttcgcggcc ctgcgtccgg accttgaaac ggccgtcctg cgggacgcgg
     2941 tgctcggcga tgttgagctg gctcatgatc ttgatacggg agacgatcgc ggacgcgtac
     3001 cctcgctggg gccgcacgat ctcctcaaga atgccgtcga cacgcatccg gacccggacg
     3061 tgattttccc agggttcaat aaaaatatcg ctcgcgcgcc gccgcacacc ttccagaagc
     3121 atcatgttca cgagctttac gaccggcgcc tggctcgcaa gttgcatgag atcggcggtc
     3181 tgttcctgct tcacgccggg agactcctcc cccgcctgtc gcgcaagggc gatcaactca
     3241 tcggtctcgg gttcggaggt tgcaaccccg gggagcttgc tgacgtcagc ggggacagcg
     3301 ccagcctggg agccgccctg atagcacttc gcgataagct ccttgatgac cgaacattcc
     3361 gccaccgtcg cgcgaatccg cttgccggac accacctgga tatcatcgat cgccttgagg
     3421 ttcgtcgggt tcgcgaatgc gacggtcagc gttttatcga gaagcgacac aggaagacag
     3481 cggtgttcct cggcggattt ctgcggaata agcgcaagaa cctcttcgtg gattacgagc
     3541 atattgaggc tgacgggacg atagccgtag agggcgctga gaaaactcac gagttttttc
     3601 tcgtcgcatt ttcccgcgtc gaggagaatg tcgatgacct gcttgcgcgg gttcttcgcg
     3661 cattccttct cggcccattc gatatcctcg gccgtcgcga tcttctcgcg cgccaggagc
     3721 gctttgatgt cgatcccaaa atccggcttt tcagtcatga cagttcccgg cgtctcattt
     3781 cttttcgtga taaagataga agatggcgtt caggatttcg gagatcctcg agataaagac
     3841 cttgggctcg cgccccgtta gctcccgcac cttcgagacg gcctcgtcgg caagggggtt
     3901 tgcgatcgcc aaaaagacac tcctggcatc cccgtcaaac gctacaacat ggtgcatgcc
     3961 acagaaatcc gcttccagga gtgacgccat gtctggattg agggcataac gcgcgagagg
     4021 gatataggg
//
LOCUS       DAOOBX010000040         4016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_25083_length_4016_cov_2.771017, whole genome shotgun sequence.
ACCESSION   DAOOBX010000040 DAOOBX010000000
VERSION     DAOOBX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25083_length_4016_cov_2.771017"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /locus_tag="PLY30_01060"
     CDS             complement(<1..1615)
                     /locus_tag="PLY30_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64719.1"
                     /translation="MRSSRRFLRCVSLVTVSCFLFTQAGFAAPIDGISVSGGAAGPEL
                     PFAGHFQVPENLGVVSETYAVTSADRSLRERAIVLVQNGHGSYDAQRKVRELLRYLDT
                     TYGIKTVFVEGAAEKLDASKIEFFADEERNRKVAEYLAEQGILNGAGLYLAEHPKGVE
                     GLGLEDVKLYRANYEALKTVFEASARVEKILSRIETRLFSTYSRYASPRLREAVADWQ
                     RFENGRREFMPFVNRLLEQVREVLGLDMRHAFSQLEWPQTVRLMALQEIEKSLDPEKA
                     AEERSSLMEFLRSRHVAGETIRTVETVFGETEPEALVDCRAVFETLLRDARPKGLTIG
                     DWPHFTAFAARRTLKRELDAPALFREIEKLFRMLTDSLAGTPEEKRFVGLQRDLALIS
                     KLLRLELTRDEYTDLRERRVSLKSLMGDIGMNVKTPARRTERGVRGKEQSGDAGGMKE
                     VFTAALRFYAFANERERVFYENIDREMRERKLTKAAVLTGGFHTDGLRGLLKESRASY
                     AVVTPHLSEASDRRLYKDAMLGKSTGGIVN"
     gene            complement(2032..>4016)
                     /locus_tag="PLY30_01065"
     CDS             complement(2032..>4016)
                     /locus_tag="PLY30_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64720.1"
                     /translation="EYWRIEQGLLSNAMAQKIFPSLIEAAKRQGSERLDDLARLMEHF
                     KRVVNPGGIVIPEGANDRDETFPYLKSRVREKTQPSEFLKRISRVERMIANARAETRM
                     SLAERIREEWPKGLNTVDRYNFFVEAGEKTIPGPNQFTYVTLPSRNEIEKGHQKPLCD
                     WTPVDLPRAESELSFWKIITKRPEQTLFKIKSSNGTDVYFQRNHAPLYPNHFLIVVDP
                     MSGRNQTVDESSLLVLLESLELIGDRDVFAGMNLGKAAGASVNSQHFQSGFMQTGRLE
                     RLEASSGVTRTPLMKVADASVTQLGGAMEPVYVVRAASKEMLARYGVLVAKSLTELDV
                     PSTLFIRPAVKGREWTMYIEPRSRVRPELFPGNAAFSEIAMGRIYGDGSVTVTDEMVA
                     EALRMAGVTSKESEILSANLKKYADADESERPSIVKAAAAEIKPFQRTSEQVTRSEVR
                     FAMTGEDGMTRQSAMNAALMEAAGAVQEAGFEGRVPLAPGARSAEIHLTELIMSLRGQ
                     GVAVPDSVSPDKGIYIAQYNKALIEQAIARRNAGIISKDVVIVALVRDRAEADFVRKK
                     FAGRIEVAVGDVNEAVATQIKLIGLLTLGNSLVQVKPGAKSADNELAYLTAALLANGR
                     IVVVGTEQTVTGAVQITVAQMVQAQLEATQSIARSA"
BASE COUNT          906 a         1236 c         1028 g          846 t
ORIGIN      
        1 ggttcacaat accgccggtg cttttgccga gcatggcgtc cttgtaaagg cggcggtcgg
       61 aagcctcgga aagatgcggc gtcacaacgg cgtagctcgc gcggctctcc tttaaaagac
      121 cccggagccc gtccgtatgg aatccgcccg tcagcacggc ggccttcgtg agtttgcgtt
      181 cgcgcatctc gcggtcgatg ttttcataaa aaacacgttc gcgttcgttc gcgaaagcgt
      241 agaaacgaag cgccgcggta aatacctcct tcatcccgcc ggcatccccg gattgttcct
      301 tcccccgtac cccgcgctcc gtcctccgcg cgggagtctt cacattcatt ccaatatccc
      361 ccatcaggga tttgagggaa acacgacgct cgcgaaggtc ggtatattcg tcacgggtta
      421 attcaagccg aagcagtttc gaaatgagcg cgaggtcccg ctgcaacccg acaaaccgct
      481 tttcctcggg cgtcccggca agcgaatcgg tcagcatgcg gaatagcttt tcgatctcac
      541 ggaaaagcgc cggcgcgtcg agttcccgtt tcagggtcct ccgcgcggca aaagccgtaa
      601 aatgcggcca atcgccgatc gtaagacctt tcggacgggc gtcgcgcaga agcgtttcaa
      661 aaaccgcgcg gcaatccacc aaagcctccg gctcggtttc accgaacacg gtctccacgg
      721 tccggatggt ttccccggca acgtgccggg aacggagaaa ctccataagc gaggatcgct
      781 cttccgcggc cttttcggga tcgagcgact tttcgatctc ctgaagcgcc atcaaccgca
      841 ccgtctgcgg ccattcaagc tggctgaacg cgtgtctcat atcgagtccc aggacttccc
      901 ggacctgctc aaggagccgg ttcacgaacg gcatgaactc gcggcggccg ttctcgaaac
      961 gctgccagtc cgcgaccgcc tcgcgaagcc ggggtgacgc gtaccgggaa taggtcgaaa
     1021 aaagacgggt ttcgatccgg gagagaatct tttcaacgcg cgcggaggcc tcgaagaccg
     1081 tcttcagggc ttcataattc gcgcggtaga gtttcacatc ttcaagaccc agaccttcga
     1141 cgcccttcgg atgctcggcg aggtaaagcc ccgcgccgtt caatattccc tgttccgcga
     1201 ggtattcggc aactttccgg ttgcgctctt catccgcgaa aaattcgatt ttcgaagcat
     1261 caagcttctc ggcggcgcct tccacgaaca ccgttttgat accgtaggtc gtatcgaggt
     1321 accggagaag ctcacggact ttccgctggg catcgtaact accgtgcccg ttctggacga
     1381 ggacgatcgc gcgttcgcgc agggaccggt cggcggaggt cacggcatat gtttcactga
     1441 ccacccccag attctccgga acctggaaat gaccggcaaa cggaagctcc gggccggcgg
     1501 cgcctccgga cacactgatc ccgtcgatcg gggcggcaaa gcctgcctgc gtgaaaagaa
     1561 aacaggaaac agtgacaaga cttacgcagc gcaggaatct acgtgatgat cgcatgggcg
     1621 attttctcct ttgaaaaacc agttttatca aaaccatcgg atgacataaa attccggact
     1681 tctaatttcc agacaggaca agcgcttccg aaaggtaacc tgcgttttga gttaccacaa
     1741 ggggcaaacg caaataaatg ctttataaat ctattaaggg cgggactgct tttgccgggg
     1801 agtgaatcgg acattcctga aacattcctc gggttattca tatgagaaca atttataaca
     1861 gatcaaaact tcttaaaacc cgctgcggaa ctccccacaa aaccacccac tccaaggccg
     1921 ggttccggaa cccgacttta gagtgggtaa aaatccaaat ctcctagatt acgtcgaccg
     1981 caccgcgaac tcaatgcccg ggtactgccg cgtacggatc aataaaatcc gttacgcgga
     2041 ccgcgcgata gattgcgtcg cctcgagctg cgcctgaacc atttgcgcca cagtgatctg
     2101 cactgcgcct gttacggtct gctccgtacc cacgacaaca atcctgccat tggcaagcag
     2161 cgccgccgtg agataggcca gctcattgtc agccgatttc gctccgggct tgacctgcac
     2221 aaggctgtta ccgagcgtca ggagaccgat cagtttgatc tgagtggcca cagcctcatt
     2281 cacatcgccc accgcgacct cgatacggcc cgcgaacttc ttacggacaa aatccgcttc
     2341 tgcccgatca cgcacaagcg caacgatcac aacatccttc gagatgatcc cggcattccg
     2401 gcgcgcgatc gcttgctcga tcaacgcctt gttatactgc gcgatataga tacccttgtc
     2461 aggactgaca gaatcaggca ccgcgacccc ctgcccccgg agactcataa tcagttccgt
     2521 cagatgaatc tcagcactac gggcgccagg agccaaagga acccgcccct cgaatccggc
     2581 ttcctgcacg gcacccgccg cctccataag tgcggcattc atcgctgatt gacgcgtcat
     2641 tccatcttca ccggtcatcg cgaatcgcac ttccgaacgg gtcacctgct cagaagtacg
     2701 ctggaacggc ttgatctcgg ccgccgcagc tttaacaatc gagggtcttt cgctctcgtc
     2761 cgcgtcggca tatttcttca gatttgcaga aagaatctcg gattctttcg aagtaacacc
     2821 ggccatccgg agggcttctg cgaccatctc atccgtgacc gtcacgcttc catccccgta
     2881 aatgcgtccc atcgctatct cggaaaacgc cgcattgccc ggaaaaagct ccgggcgaac
     2941 acgtgaacgg ggctcgatat acatcgtcca ttcacgacct ttgaccgcag gacggataaa
     3001 gagcgtcgaa gggacgtcaa gttctgtcag agacttcgcg acaaggactc catagcgggc
     3061 caacatttct ttggaagccg cgcgaaccac ataaacaggt tccatggcgc ctccaagctg
     3121 tgtgaccgaa gcatccgcga ccttcataag aggcgtgcgc gtaacgcccg acgaagcttc
     3181 cagacgttcc agccgaccgg tctgcataaa cccgctctgg aaatgctgtg aattcaccga
     3241 tgcgcccgcc gccttgccaa gattcattcc agcaaacaca tcgcggtccc cgataagctc
     3301 gagcgattcc agcagaacca gaagcgacga ctcgtcaaca gtttggttcc ggcccgacat
     3361 cggatcgacc acgatcaaaa agtgattcgg ataaagcggc gcgtggttac gctgaaagta
     3421 cacatccgtg ccgttagagc tcttgatttt gaacaaggtc tgctcgggcc gcttggtgat
     3481 gatcttccaa aagctcagct ccgactctgc ccgcggcaga tcgaccggcg tccagtcaca
     3541 gagaggcttt tggtgtcctt tttcgatctc attccgtgaa ggcaatgtca catacgtaaa
     3601 ctggttggga ccggggatag tcttctcccc cgcctccaca aagaagttat atcggtcaac
     3661 cgtattaagg cccttcggcc attcctcgcg aattctctca gcaagcgaca tccgcgtttc
     3721 ggcgcgcgcg ttggcgatca ttcgctcaac tcggctgatg cgcttcaaaa actcactcgg
     3781 ctgagtcttt tcacggaccc tggacttcaa gtacgggaag gtttcatccc tgtcatttgc
     3841 gccttcgggt atgacaatcc ccccgggatt cacgacacgc ttgaaatgct ccatcaacct
     3901 ggcaagatcg tcgagccgtt cggagccctg ccgcttggcc gcttcaatca gggacgggaa
     3961 gatcttttgg gccattgcat tactgagaag accttgttcg attcgccaat attcat
//
LOCUS       DAOOBX010000041         4007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_25150_length_4007_cov_3.199140, whole genome shotgun sequence.
ACCESSION   DAOOBX010000041 DAOOBX010000000
VERSION     DAOOBX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25150_length_4007_cov_3.199140"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLY30_01070"
     CDS             complement(<1..696)
                     /locus_tag="PLY30_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64721.1"
                     /translation="MGSAAHRQLSFAAGMTVAALLVCGIFHALGELRTTFQPEIQLQR
                     IAIMFAGLMIPVLTTWWRFKWVGVNCMAVLLLVPLVFFVVSVTKIPVFLWSFFAYAAL
                     MLVLCRAEQKSDTQLSMILIDEEKVQDEINNLEIVFKAKGEGISTFFEKYSTYYNLRK
                     LAEELALSLAWTDLAQKTVDRAMDFIPRGDYAKVTLARGDERNLPIIARRRMRPVGHW
                     TAKQGDLFDFWCIK"
     gene            complement(713..1819)
                     /gene="ugpC"
                     /locus_tag="PLY30_01075"
     CDS             complement(713..1819)
                     /gene="ugpC"
                     /locus_tag="PLY30_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPW64722.1"
                     /translation="MANVTLKNISKIYKGGVRAVNNISLEIKDREFVVLVGPSGCGKS
                     TTLRMIAGLEAISEGEIFIDNILVNDVPAKNRDIAMVFQNYALYPHMTVYENLAFGLK
                     LRRYPRREIERRVHEAAEILGLSLDMLQRRPKELSGGERQRVAVGRAIVRKPKVFLFD
                     EPLSNLDAKLRVQMRMELQKLHNRLQSTMIYVTHDQIEALTLGDRIVVMHKGIVQQVA
                     DPVSLYERPYNRFVGGFIGSPPMNFLKGTIMAENGDFYFYDKSIKLRILPHYYPRMST
                     YKGQPVLMGIRPEDIYDKIFAQDARPEFTITATVDVVEPMGAEIFLYLTSGNNNFVVR
                     VSNQDTAAMNQDLQVVFDMSKAHFFDPQTGVTIF"
     gene            complement(1824..2684)
                     /gene="accD"
                     /locus_tag="PLY30_01080"
     CDS             complement(1824..2684)
                     /gene="accD"
                     /locus_tag="PLY30_01080"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPW64723.1"
                     /translation="MTDKKEKPMAQAPALLVTKGLRRKSIPEGLWTKCPSCQTVIFNK
                     TLKDNLLVCTKCDYHFIMGAWDRIHCIVDDKTFTESDANLKPGDPLKFSAVKSYPEKV
                     KQDQKKTGLREACITGQGKINGHSIAIGVTDSRFIMGSMGSVVGEKITRLTEYALEHK
                     LPLIVISGSGGGARMYEGALSLMQMAKTSVALARYQKGRKPFISILTNPTMGGVMASY
                     ASLGDIIIAEPRALLGFAGPRVIEQTIRQKLPADFQTSEFLVDHGMVDMVVRRCDLKQ
                     KLTTLLEILC"
     gene            2872..>4007
                     /locus_tag="PLY30_01085"
     CDS             2872..>4007
                     /locus_tag="PLY30_01085"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64724.1"
                     /translation="MKTNMKLSQQQRLVLSPQMREYLRLLHLPVAELRQAVDKAIEEN
                     PMLEEAASPETAEAPVPSMESTREDEGNLSRAVTGDWDDAESINFFKSPTDFSKRPAA
                     DMQHLKDFQDSLLTRPESLFNFLEWQIQFLAFTGNDKTIAGQIMGNINEDGYLTATIG
                     EIARAAEALPEDVERVLAEIQKLDPPGVGARDLKEALLIQLSRRGPGAGLAREIVLHH
                     LDLVAKKDWRSLARIFRQSEAAIRSAVEMISRLEPRPGRSFYAEEAITVTPDATISIR
                     DGSDNDLEIRVNDEFIPRLRINAEYRKMLRDRGTDRSTRDFLKTRLSNGMDFIKALEL
                     RKSTIRSITEEIVKAQPLFFTRGFSHLRPLRMKDIAERLDLHEST"
BASE COUNT          877 a         1131 c         1163 g          836 t
ORIGIN      
        1 cttgatgcac cagaaatcga aaaggtcccc ctgcttggcg gtccagtggc cgacgggccg
       61 catgcggcgg cgcgcgatga tcggcaagtt ccgttcgtcg ccgcgggcga gcgtgacctt
      121 ggcgtagtcg ccgcgcggga tgaaatccat cgcccggtcc acggtcttct gcgcaagatc
      181 ggtccaggcc aggctgagcg caagctcctc ggcgagtttg cggaggttgt agtaggtcga
      241 atatttttcg aagaaggtgc tgataccttc acctttcgcc ttgaacacga tctcgagatt
      301 attgatctcg tcctggactt tctcctcgtc gatcaggatc atggaaagct gggtgtcgga
      361 tttctgttcg gcgcggcaaa ggacgagcat gagggccgca taggcgaaaa aggaccagag
      421 gaacacggga atcttcgtca ccgagacgac gaagaacacg agcggcacaa ggaggaggac
      481 ggccatgcaa ttgacgccca cccacttaaa ccgccaccag gttgtgagca ccggtatcat
      541 caaacccgcg aacatgatag cgatacgctg gagttgaatc tcgggctgga acgtggtgcg
      601 aagctcgccg agcgcgtgga agatgccgca gactaacagc gccgcaacgg tcatccccgc
      661 tgcgaacgat agctgacgat gcgcggctga gcccataaga ggtcagaacc cctcagaaga
      721 tcgtgacgcc cgtttgagga tcgaaaaagt gcgctttgct catatcgaac accacctgca
      781 ggtcctggtt catcgcggcg gtgtcctggt tcgagacgcg gacgacaaag ttgttattcc
      841 cgctcgtcag gtacaggaaa atctcggcgc ccatcggctc cacaacgtcg accgtcgcgg
      901 tgatcgtgaa ctcggggcgc gcgtcctgag cgaaaatctt gtcgtagata tcctcaggac
      961 ggattcccat cagcacgggc tgccccttgt aggtcgacat gcgggggtaa taatgcggca
     1021 ggatccggag cttgatactc ttgtcgtaga aataaaaatc cccgttctcg gccatgatcg
     1081 ttcccttgag aaaattcatc ggaggcgatc cgatgaatcc gccgacgaac ctgttgtagg
     1141 gacgctcgta cagactcacg ggatccgcga cctgctggac aatccctttg tgcatgacaa
     1201 cgatacgatc cccgagggtc aacgcctcga tctggtcgtg cgtgacgtag atcatggtcg
     1261 attgaagccg gttgtggagc ttctgaagct ccatgcgcat ctgcacgcgc agttttgcgt
     1321 caagattgct cagcggttca tcgaacagaa agaccttggg tttgcggacg atcgcacgtc
     1381 cgaccgcaac gcgctggcgc tcgccgcccg aaagttcctt gggacgccgt tgcagcatat
     1441 ccagcgagag gccgagaatc tcggccgctt catggacgcg gcgctcgatc tcgcgtctgg
     1501 ggtaacggcg cagtttgagc ccgaacgcga ggttctcata gaccgtcatg tgcggataga
     1561 gcgcgtaatt ctggaacacc atcgcgatat cgcggttctt cgcggggaca tcgttgacga
     1621 ggatgttgtc gatgaaaatc tcgccttccg agatcgcttc gagccccgcg atcatacgga
     1681 gcgtcgtgga cttgccgcag ccggaggggc cgacgagaac cacgaactcg cggtccttga
     1741 tctcgaggct gatattgtta acggctcgca cgcccccctt ataaatcttt gagatgttct
     1801 tgagggtgac gttcgccatg atgtcagcag agaatctcga ggagcgtggt caatttctgc
     1861 ttcaggtcgc aacgacggac gaccatgtcc accatgccgt ggtccacaag gaattcggag
     1921 gtctggaaat ccgcgggcag tttctggcga atcgtctgct cgatcacgcg ggggcccgca
     1981 aaaccgagca gcgcgcgcgg ttccgcgatg atgatgtctc cgagggacgc gtagctcgcc
     2041 atgacgccgc ccatggtcgg gttcgtcagg atcgagatga aaggtttccg tcccttttga
     2101 tagcgtgcaa gggccaccga ggtcttggcc atctgcatga ggctgagggc cccttcgtac
     2161 atgcgtgcgc caccgcctga gcccgaaatg acgatcagcg gcagtttgtg ctcaagggca
     2221 tactcggtca gccgggtgat cttctccccg accacggaac ccatggaacc catgatgaac
     2281 cgggaatccg tgacaccgat cgcgatgctg tggccgttga tcttcccctg gccggtaatg
     2341 caggcttccc tgagtccggt cttcttctgg tcctgcttga ccttttcggg ataggatttg
     2401 acggccgaga atttcagcgg atcaccgggc ttgaggttgg catcggattc ggtgaaggtc
     2461 ttgtcgtcga cgatgcaatg aatgcggtcc cacgcgccca tgatgaagtg ataatcgcat
     2521 ttggtgcaaa cgaggagatt atccttgagc gtcttgttga aaatgacggt ctggcagctg
     2581 ggacatttgg tccaaagtcc ttcggggata cttttccgtc tcaatccttt tgtaacgagt
     2641 aatgcggggg cctgcgccat cggtttttcc tttttatcgg tcacatgatt ccggacggtt
     2701 ggaccataca aaaaagccac gagagagagc gacttgcctg tcccgcggcc gttcccggta
     2761 agcgaatggc tacatgataa aaaaatattt ttacagtgtc aatcggtttc tccctctaac
     2821 ctttgaaatc tcatggcttt acaacatttt atcaccatgc tattttgcag catgaaaacg
     2881 aacatgaagc tttcacagca gcaaaggctc gtcctttccc cgcaaatgcg ggaatatctg
     2941 agacttttgc atctccccgt ggccgaactc cggcaggccg tggataaagc gatcgaagag
     3001 aatcccatgc ttgaagaagc cgcgtcgccc gaaacagcgg aagcgccggt cccctctatg
     3061 gaaagcaccc gggaggacga agggaatctc tcgcgggcgg tcacgggtga ctgggacgat
     3121 gcggaatcaa tcaatttctt caaatctccg acggattttt ccaaacgccc ggcagcagac
     3181 atgcaacatc tcaaagactt ccaggatagc ctgctgaccc gccccgaaag cctcttcaat
     3241 ttcctggaat ggcagatcca gttccttgcg ttcacgggta acgacaagac gatcgccggt
     3301 cagatcatgg gaaatatcaa cgaggatggc tatctgaccg cgaccatcgg cgagatcgcg
     3361 agagcggcag aagctttgcc cgaggatgtc gaacgcgtgc tggcggagat acagaaactt
     3421 gatcctccgg gcgtcggcgc ccgcgatctg aaggaggcgc ttttgatcca gctaagccgc
     3481 cgcgggcccg gcgcggggct tgcccgcgag atcgtcctgc atcacctgga tctcgtcgca
     3541 aaaaaagact ggcgctcgct cgcgaggatc tttcgccaga gcgaggccgc gatcaggagc
     3601 gccgttgaaa tgatctcgag gcttgagccc cgtccggggc gcagctttta cgcggaagaa
     3661 gcgatcacgg tcaccccgga cgccaccatc tcgatcaggg acggtagcga caacgatctc
     3721 gaaatccggg tgaacgatga gttcatcccc cgtcttcgca tcaacgcgga ataccggaaa
     3781 atgctccgtg accgcggcac cgatcgttcc acgcgggatt tcctgaagac gcggctttca
     3841 aacggcatgg atttcatcaa ggcgctggaa ctccgcaaga gcacgatccg cagcatcacc
     3901 gaagaaatcg tgaaggccca gccgcttttc ttcacgcgcg gattctccca tctccggccg
     3961 cttcgcatga aggatatcgc cgaaaggctc gatctccacg aatcaac
//
LOCUS       DAOOBX010000042         3993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_25271_length_3993_cov_3.385221, whole genome shotgun sequence.
ACCESSION   DAOOBX010000042 DAOOBX010000000
VERSION     DAOOBX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25271_length_3993_cov_3.385221"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1335
                     /locus_tag="PLY30_01090"
     CDS             253..1335
                     /locus_tag="PLY30_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HPW64725.1"
                     /translation="MKSYRVTLIPGDGVGPELAEAAKACLEATGVKFDWDIQTAGVDI
                     MEKEGTPLPGRVIESIRRNKIAIKAPITTPVGKGFRSVNVALRKELDLYACLRPCKSY
                     EGVRSRYRNIDLVIVRENTEDLYAGIEFRKGTSEALRLIDKIEALSGRKIRPDSGVSI
                     KPISVSGTERIVKFAFDYARQYKRKKVTAVHKANIMKFSDGLFLEVAREVAKQYSDIE
                     FEDRIVDNMCMQLVQKPELYDVLVLPNLYGDILSDLCAGLVGGLGVAPGANIGTNGAL
                     FEATHGSAPKYKGQNKLNPTAVILSGVLMLRHLGETEAADRLENAVAAVIREGKDVTY
                     DMKADRNDPAAVGTREMGEAIIRKMK"
     gene            1347..>3993
                     /gene="acnA"
                     /locus_tag="PLY30_01095"
     CDS             1347..>3993
                     /gene="acnA"
                     /locus_tag="PLY30_01095"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HPW64726.1"
                     /translation="MSLLNSFGSKVTLNIEDRDYTIYRVRALQECGVAKVLKLPYAIR
                     ILLENLLRNEDGKNVTRGDIENLANWSPSKKSAKEIAFTPARVLLQDFTGVPAVVDLA
                     AMREAMVKMNGDPKKINPLQPVDLVIDHSVQTDFFGTPDSFRKNGEKEFERNVERYQF
                     LKWGQKAFNNFRVVPPATGIIHQVNLEHLSPLVFRRKEGGKTYLYPDTLVGTDSHTTM
                     INGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLTGKTREGVTITDVVLTVTQML
                     RKKGVVGKIVEYFGDGVGALSLADRAVISNMAPEYGATAGYFPVDEETIRFLRLTGRN
                     HEAVDLAEVYFKEQGLFRENPAEAVQYSDILELDLKTVESSLAGPRRPQDRIALKEVK
                     ASFEKVLPDLLKGKGVLDRKMSIEIEGQKCELENGAVVIASITSCTNTSAPSAMLTAG
                     LVAEKAILKGLMVKPWVKTSLAPGSQVVTDYLRDSGLLYYFEKLRFHLTGYGCMTCIG
                     NSGPLPEPVSWAIKEGDLVAASVLSGNRNFEGRVHPQVKANYLASPALVVAYAIAGTV
                     LIDMEKDPLGKDKDGKPVFLKDIWPTAEELREAMNSVKPEMYKNRYKNAFEGNEAWKA
                     LVASSGDTFEWDRKSLYVKHPPYFQKMPARPSDIRDIRDARVLAVLGDSITTDHISPA
                     GSIKTDSPAGEYLIENGVDPKDFNSYGARRGNHEVMVRGTFANIRLKNLMIPGVEGGM
                     TKYFPERGRPSDVTIYDASVKYQERKVPLVVIAGKEYGSGSSRDWAAKGPALLGVKAV
                     IAESFERIHRSNLIGMGILPLQFIEGESAASLKLSGEEVFAIEGIADQLSVGKILSCT
                     ADRKTFKVICRIDTPDAAVGDGLEDK"
BASE COUNT          883 a         1107 c         1209 g          794 t
ORIGIN      
        1 cgttgttctt taccgaaggc ggcccggacg accttgcccg ccttaccgat cgcttccaat
       61 agggtgcttt taattttggt gctattcata tagaatacgg gtcctttcag tgggtttttc
      121 agcaggagat ttgtatttta gccgaatggc tggggcgctg caactcattg aacggcacaa
      181 ccttgataag cgaaagcgga actactttca tcctgcgggg cgaagcggtt cggcggaaat
      241 cgggagcaac aaatgaaatc ttaccgtgtg acactgatcc ccggcgacgg cgtcggtccg
      301 gagctcgcgg aagcggcaaa agcgtgtctt gaggctaccg gcgtcaagtt cgactgggat
      361 atccagacgg caggcgtcga tatcatggaa aaggaaggaa ccccgcttcc tggccgcgtc
      421 atcgagtcga tccgccggaa caagatcgcg atcaaggcgc cgatcaccac acctgttggc
      481 aaggggttcc gttccgtgaa tgtcgcgctt cgcaaggaac tcgatctcta cgcgtgtctc
      541 cgtccatgca aatcttacga gggcgtccgt tcgcgctaca ggaacatcga tctcgtgatt
      601 gtccgcgaga ataccgaaga cctttacgcc ggcatcgaat tccggaaagg aacctccgag
      661 gcgctccggc tgatcgacaa gatcgaagcg ctttccgggc gcaagatccg tcccgattcc
      721 ggtgtcagca tcaagccgat ctccgtttcc gggaccgagc gcatcgtgaa gttcgcattc
      781 gattatgccc gtcagtacaa acggaagaag gtgaccgcgg tccataaggc aaacatcatg
      841 aaattctcgg acgggctctt cctcgaggtc gcccgtgagg tcgcgaaaca atattctgat
      901 atcgagttcg aggaccgcat cgtcgacaat atgtgcatgc agcttgtgca gaaacccgaa
      961 ctttacgatg tccttgtgct tccgaatctc tacggcgata tcctttccga cctctgcgcg
     1021 gggctcgtcg gcggtctcgg cgtggcgccg ggagccaata tcggtacgaa cggcgcgctc
     1081 ttcgaggcga cgcacggttc ggcgccgaaa tacaaaggtc agaacaagct gaacccgacc
     1141 gccgtgatcc tctcgggcgt cctgatgctg cggcatctcg gtgagaccga agcggctgat
     1201 cgcctcgaga acgcggtcgc ggccgtgatc cgcgaaggca aagacgtgac gtatgacatg
     1261 aaggcggacc ggaacgatcc ggccgccgtc ggaactcgcg agatgggcga agcgattatc
     1321 aggaaaatga aataaccggg aaagccatga gccttcttaa cagtttcgga agcaaagtca
     1381 cgctgaacat cgaagacagg gattacacga tttaccgggt ccgcgcgctc caggaatgcg
     1441 gcgtcgcgaa ggtcctgaag ctcccgtacg cgatccgtat cctgcttgag aacctgcttc
     1501 gaaacgagga cggcaagaac gtcacgcgcg gcgatatcga gaacctcgcc aattggtcac
     1561 cttcgaaaaa gagcgcgaaa gagatcgcgt tcacgccggc ccgcgtcctc ctccaggatt
     1621 tcaccggggt tccggctgtc gtcgaccttg ccgcgatgcg cgaggcgatg gtcaagatga
     1681 acggtgaccc gaaaaaaatc aatcccctcc agccggtcga tcttgtgatc gatcactcgg
     1741 tccagacgga ttttttcgga acgccggatt ccttccgcaa gaacggcgaa aaggagttcg
     1801 agcgtaacgt cgaacgctat caattcctca aatgggggca gaaggctttt aataacttcc
     1861 gggtggtgcc gccggccacg gggatcattc accaggtgaa tctcgagcat ctctccccgc
     1921 ttgttttccg gcgcaaggaa ggaggcaaga cctatcttta tccggatacg ctggtcggta
     1981 ccgattccca tacgacgatg attaacggcc tcggcgttct cggctggggc gtcggcggga
     2041 tcgaggccga ggccgcgatg ctcggacagc ctgtttcgat gctgatcccg gaagtgatcg
     2101 gcttcaagct gacggggaaa acaagggagg gggtcacgat cacggacgtg gtactgacgg
     2161 tcacgcagat gcttcgcaaa aaaggcgtgg tcggcaagat cgtcgaatac ttcggcgacg
     2221 gcgtcggggc tctttcgctt gcggatcgcg cggtgatttc caatatggcc cccgagtatg
     2281 gcgccacggc ggggtatttc cccgtcgatg aggagacgat cagattcctt cgactgacgg
     2341 gaagaaatca tgaggctgtg gacctcgcgg aggtctattt caaggaacag gggcttttcc
     2401 gcgaaaatcc ggccgaggcg gtccagtatt cggatatcct tgagcttgac ctcaagacgg
     2461 tggaatcgag cctcgcgggg cccaggcgcc cccaggaccg gatcgcgctg aaagaagtca
     2521 aggcgtcatt cgaaaaggtt ttgcccgatc tgttgaaagg aaagggcgtt ctcgacagga
     2581 agatgtcgat cgagatcgaa ggccagaagt gcgagctgga gaacggcgcg gtcgtgatcg
     2641 cttcgatcac aagctgtacc aacacctccg ccccgtccgc gatgttgacg gccggtctcg
     2701 tggccgagaa ggcgatcctg aaaggtctca tggtcaagcc gtgggtgaag acgagcctcg
     2761 cgcccggttc ccaggtcgtg acggattatc ttcgggactc cggcctgctt tattatttcg
     2821 agaagttgcg gtttcacctt accgggtacg gatgcatgac ctgtatcggc aactccggtc
     2881 cattgccgga acccgtttcg tgggcgatca aggaaggcga tcttgtggcc gcctccgtgc
     2941 tttcgggaaa tcgtaatttc gaggggcgcg tccatcccca ggtgaaggcc aactacctcg
     3001 cctcgccggc acttgtcgtg gcgtacgcca ttgcggggac cgttctgatt gatatggaaa
     3061 aggatcctct gggcaaagac aaggatggca agccggtctt tctgaaggat atctggccga
     3121 ccgcggagga acttcgagaa gcgatgaaca gcgtgaaacc cgagatgtac aagaaccgct
     3181 acaagaacgc gtttgaaggg aacgaggcct ggaaggccct ggttgcgtcc tcgggagata
     3241 cgtttgagtg ggacagaaag tccctttacg tgaaacaccc gccctatttc cagaagatgc
     3301 cggcgcgtcc ttccgatatc agggatattc gcgacgcgcg cgtgctggcc gtccttggcg
     3361 actcgatcac gaccgatcac atctcgccgg ccggatccat caagacggac agtccggccg
     3421 gggaatacct gatcgagaac ggcgtcgatc ccaaagattt caactcctac ggcgcccggc
     3481 gcggcaacca cgaagtgatg gtccggggca ccttcgcgaa tatccgcctt aagaatctca
     3541 tgatccccgg cgttgaaggc ggtatgacga aatatttccc ggaaaggggg aggccctccg
     3601 acgtgacgat ctacgacgcg tcggtgaaat accaggagcg caaggtcccg ctcgtcgtga
     3661 tcgcgggcaa ggaatacggc tccggctcgt cccgcgactg ggcggcaaaa ggcccggccc
     3721 ttctcggagt gaaggctgtg atcgcggaaa gctttgagcg catccaccgc agcaacctga
     3781 tcgggatggg catcctgccg ctccagttca tcgaaggcga aagcgccgct tcactgaaac
     3841 tttcgggcga agaggttttt gccatcgaag ggattgccga ccagctcagt gtcggaaaga
     3901 tcctgagttg caccgcggac aggaaaacgt tcaaggtcat ttgccggatc gatacccctg
     3961 atgccgctgt cggcgatggc ttggaagaca agg
//
LOCUS       DAOOBX010000043         3969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_25444_length_3969_cov_2.910066, whole genome shotgun sequence.
ACCESSION   DAOOBX010000043 DAOOBX010000000
VERSION     DAOOBX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25444_length_3969_cov_2.910066"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1468)
                     /locus_tag="PLY30_01100"
     CDS             complement(254..1468)
                     /locus_tag="PLY30_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW64727.1"
                     /translation="MNSQKSKALRLILLFGCVSLFGDIIYEGARSVNGPYLKVLGANA
                     LLVGVIAGIGEFLGYTIRLFSGYLSDKTRSYWFFTILGYALIVSVPLLAFTSLWHVAA
                     LLIVAERIGKALRSPAKDTILSQATKQVGTGFGFGLNEMMDQIGALAGPLIFTFFFLS
                     KGAGDRTLADYQHGYGLFWIPFALLMLCVLFAWRSVPDPAALEPAKTNEPDKLPKVFW
                     TYTVFSFLTTMGFVNFVLLAFHYKTRQVLSDMEIPLYYAIAMGVDGAAALGVGKLYDI
                     LKEKNGHDHGGLATLIVIPVLSALIPFLAFSGSRWVVAAGIILWGIVMACHETIMKSA
                     IADITPLRKRGTGYGVLNTAYGLAMLTGGVLTGYLYDRSLPLLIGATVTLQIFSLPFF
                     MSIQKSVAETKT"
     gene            1838..2521
                     /locus_tag="PLY30_01105"
     CDS             1838..2521
                     /locus_tag="PLY30_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPW64728.1"
                     /translation="MQLTFRKIEKAFEVDEKTLYQWLNDRGMPAVKANGQYLFNSVEV
                     LEWALKHRIPLTLGALKLCERNRPGQDILTPALLRGGVHTGVKGERRDEIFGEVLNAL
                     PLPASVDKFSLKAMLISREQVGTTGIGNGIAIPHVKHPVIFAGLDPIVGLFFLEKPVN
                     FAAPDGERVHTLFVILSGSFRGHLSLLSRLAFCLQDPSVKTALARRASPEEILTAFQV
                     AEAKTGGGS"
     gene            2522..>3969
                     /locus_tag="PLY30_01110"
     CDS             2522..>3969
                     /locus_tag="PLY30_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPW64729.1"
                     /translation="MEKIFRGPWTVPFGECSLRLDPLNLVFLAAIVVLVLSSAIYGTG
                     YLRPYRGKKPLGIHAFFYLVFMAAMVLIVTAGNAILFLGAWEVMTLSTFFLIIFDDEK
                     PAVRQAGFIYLIASHCATFFLFLMFFLMAHAAGSMDFDVMARTDFPQAVAALAFLLAL
                     AGFGVKAGFLPTHIWLPYAHPAAPAHISALLSGIAIKMGIYGICRVLWIVKTLPGWCG
                     YTLLALGIVSGLMGVLYALGQHELKRLLAYHSIENIGIIAVGLGVGLLGRSHQVPFLA
                     VLGFGGALLHVVNHALFKGLLFLGAGSVIHRTHSGEMDHLGGLAKTMPWTAGFFLAGA
                     LSICGLPLFNGFVSEFLIYFGLFTGLRELPPEGVVACSLGILSLALMGALALACFSKV
                     YGIVFLGKPRTDIKTDGTPEGTPFSMLLPMAILAALCLWIGLVPQSVTSLIFGGGTYL
                     AHTDAFAVRLGTVLGPLAPVVRMALLFLALILG"
BASE COUNT          746 a         1142 c         1188 g          893 t
ORIGIN      
        1 cgggaggccc tgctcaaagc caaggcttcc ggcaagaacc agaccgccat tcagaacagc
       61 tgaacgtttt tatcccgagc tgtcccatcc ttatcgcttc cggagatgaa tgcgaaaagc
      121 gccctgggcg cggctggccg ggccgtctct gtccttgtga aagccaaagc ctccccgaaa
      181 tcccccgcct gctttcagga aggtcccgcg gttccccggg aggattcccg accggaaaat
      241 caccgtttgg cggttacgtc ttcgtttcgg caacgctttt ttggatcgac atgaagaagg
      301 gaagagagaa tatctgcagt gtgaccgtcg cgccgatcag aagagggagg gaccggtcgt
      361 acagataacc cgtcaagaca cctcccgtca gcatcgcgag gccgtaggca gtattgagaa
      421 ccccgtaacc cgtgcctctt ttccggagcg gcgtgatatc tgcgatcgcg gatttcatga
      481 tggtctcatg gcaggccatg acgatgcccc agaggatgat cccggcggcc acgacccacc
      541 gcgatcccga gaaagcgagg aaggggatca gggccgaaag gaccgggatc acgatgagcg
      601 tcgcgagtcc tccgtgatcg tggccgttct tttctttcag gatatcgtag agttttccga
      661 cgccgagcgc cgcggcgccg tccacgccca tcgcgatcgc gtaatagagc ggaatctcca
      721 tgtccgagag tacctgccgt gttttgtagt ggaaggcgag caggacgaag ttcacgaaac
      781 ccatggttgt gagaaaactg aacactgtat aggtccagaa gaccttcggg agcttgtccg
      841 gctcgttcgt tttcgcaggc tcgagcgccg cggggtcagg gacactccgc cacgcgaaga
      901 gcacgcagag catcaggagc gcaaagggga tccaaaagag accgtagccg tgctggtagt
      961 ccgcgagcgt ccgatccccc gcgcctttcg acaggaagaa aaacgtgaag atgagcggac
     1021 cggcgagcgc gccgatctga tccatcatct cgttcagccc gaagccgaag ccggtgccga
     1081 cctgtttggt ggcctgtgac aggatcgtgt ctttggccgg gcttcggagg gccttcccga
     1141 tccgttccgc cacgatcagg agcgccgcga cgtgccagag ggacgtgaac gcgaggagcg
     1201 gcaccgagac gatcaacgcg tagccgagga tcgtgaagaa ccagtaggaa cgcgtcttgt
     1261 cggaaagata gccggaaaaa agacggatcg tgtacccgag gaactcaccg atccccgcga
     1321 tgacgccgac gagaagagcg ttcgcgccga ggaccttgag gtagggaccg ttgacgctgc
     1381 gcgcgccctc ataaatgatg tcaccaaaga gactgacgca tccgaagagg aggatgagac
     1441 gaagtgcctt gcttttttgg gagttcatgg caacaaagct tactgatttt aacaggggaa
     1501 tcaaatagaa tgtatgacat gccatacgcc acacggcgaa taccgcgaaa agccatgagg
     1561 gggcattgac agtttttata tttgctctag tatgcgtgaa tacacgtaga cgcgtatggc
     1621 aagaaatctt ttggtcttac gatctgtaat gtccgcttca tcagcccgca aagccagctt
     1681 ggcaaatgat cggctgtctg tgatgagatt aacatgagtg aacggatcgt tcagggtccg
     1741 cggcgtctcc aaccttgtat caaatcattt tgtgccatga agtaacaccg ggccggggag
     1801 ccttgttttt cgggtctctc accggacagg aaatgatatg cagctgactt tccgcaagat
     1861 tgaaaaggct ttcgaggtcg atgagaaaac gctctaccag tggctcaacg accggggtat
     1921 gccggcggtc aaggccaacg gacaatacct gttcaattcc gtggaagtcc tggaatgggc
     1981 cctcaaacac cgcatccccc tgacccttgg ggccctgaaa ctctgcgaaa ggaatcgccc
     2041 gggccaggat atcctgacgc ccgccctcct ccgcggcggg gttcataccg gcgttaaagg
     2101 ggagcgacgc gacgagatct tcggggaagt tctgaacgcc cttccgcttc cggcctccgt
     2161 cgacaagttt tccctgaagg cgatgctcat ctcccgcgag caggtcggca cgacgggcat
     2221 cgggaacggt attgccatcc cgcacgtcaa acatcccgtg atctttgccg gcctggatcc
     2281 gatcgtggga cttttttttc tcgaaaagcc ggtgaatttt gccgcgccgg acggagagcg
     2341 ggttcacacc ctgttcgtga tcctgtccgg ttccttcagg gggcatctgt cgcttctctc
     2401 ccggttggct ttttgtcttc aggacccctc cgtgaaaacg gccctggccc gccgcgcaag
     2461 ccccgaagaa atccttaccg cgttccaggt tgcggaagca aaaacaggcg ggggctccta
     2521 agtggaaaaa atcttccggg gcccctggac ggttccgttt ggcgaatgtt cgctccgcct
     2581 ggatcccctg aacctcgtct tcctcgcggc gatcgtggtt ctcgttttgt cgagcgccat
     2641 ctatggcaca ggttacctgc gtccttaccg gggaaagaag cctctcggga tccacgcgtt
     2701 tttctatctg gtttttatgg cggctatggt ccttatcgtc accgccggga acgcgattct
     2761 ttttctcggc gcctgggagg ttatgaccct ttcgacgttc ttcctgatta ttttcgacga
     2821 tgaaaaaccg gcggtgcggc aggcgggatt catctatctg atcgcgtccc attgtgcgac
     2881 gttcttcctt tttctgatgt tctttttaat ggcccacgcg gcgggttcga tggattttga
     2941 cgtgatggcg cgaacggatt tcccgcaggc cgtcgcggcg ctggccttcc tcctggcgct
     3001 ggccggtttt ggcgtcaagg cgggattcct tccgacgcat atctggctgc cgtacgcgca
     3061 cccggcggcc ccggcgcata tctccgcgct tttgtcgggg atcgcgatca agatggggat
     3121 ctacgggatt tgccgggtcc tctggatcgt gaagaccctt cccggctggt gtggttacac
     3181 gcttttggcc ctcggcatcg tttccggtct gatgggcgtt ctgtacgcgc tcggccagca
     3241 cgagctgaaa cggttgctcg cctatcacag catcgagaat atcggcatca tcgcggtcgg
     3301 acttggcgtc ggactgttgg gtcgcagcca tcaggtcccg tttcttgcgg tcctggggtt
     3361 cggcggggcg cttttgcatg ttgtgaatca cgcccttttc aagggtcttc ttttcctcgg
     3421 ggcggggtcc gtcatccacc ggacgcattc cggcgagatg gatcacctcg ggggcctcgc
     3481 gaagacgatg ccctggaccg ccggtttctt tctcgcgggg gccttgtcga tctgcggact
     3541 gccgctcttt aacggtttcg tgagcgagtt tctcatttat ttcggtcttt tcaccgggct
     3601 ccgggaactt ccgcccgagg gggtggtcgc ctgttcgctc gggatccttt cgcttgcgct
     3661 gatgggggcg cttgcgctgg cctgtttcag caaggtctac gggattgttt tcctgggcaa
     3721 gccccggacc gatatcaaaa cggacggcac gcccgagggc acgccgtttt caatgctcct
     3781 tcccatggcg atcctcgcgg ccttatgcct ctggatcggt cttgtgccgc agtccgtgac
     3841 gagcctgatc tttggcggcg gaacttatct cgcgcatacc gatgcctttg cggtcaggct
     3901 cgggactgtc cttggtccgc tcgcgccggt ggtccggatg gcgctccttt tcctcgcgct
     3961 gatcctggg
//
LOCUS       DAOOBX010000044         3913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_25960_length_3913_cov_2.955158, whole genome shotgun sequence.
ACCESSION   DAOOBX010000044 DAOOBX010000000
VERSION     DAOOBX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25960_length_3913_cov_2.955158"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3913)
                     /locus_tag="PLY30_01115"
     CDS             complement(<1..>3913)
                     /locus_tag="PLY30_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64730.1"
                     /translation="KKLDLFTYLSRTVALADSQKLHTRRPALHVEGLLRFILDAVKSN
                     DPVVIGKAKHIDAREVFTELLRLERSIAEACLPEAVDRELYGHYRTLRLVERLNDLMV
                     TQEEYAALRVSLEPGGKSPGQTSSPGPGLPAYFDTQSLGEFIHKHAKKPLVLSKQWER
                     NIRDAVKFYEIAAERDGALSEALDRHFRKNPRTPHSKLPGSEPAILVYGGFHKENVKR
                     ILEARGIGYVVVSPGITKPSPRHEKIYRKLMTEGMLPFEQPVPPALGRASRPIPVTVL
                     PNGREAIRRVCDALRFNPEADPETLGLVLGAQTRRGKSRAPEKSDIRQTLDRDAKLQG
                     IAEALKPVLKEKDARMATARQILDLAKALPAGSKLALEDLKAVSALQSVSDEKLRQIF
                     DLAAEPSRAPPGQARDSRVPRRRMFVFAGAAALAGIAGAALFWSETKKTARVRSTVDA
                     LTSYYRENYPRFHIQPPMIVQPVNHVSKSWEGYRLELIAGPHGAPKAAEKILELAPLL
                     AESPEKWQIVLEGGVNTLTRSGRLADVTVLIKLSDLLRIPVVDPVPTPSDPEVIREIE
                     KRLSLTKDEVMAANLFDTLFFLAGEAGMSGKSSRQAWETFLTYRSMTCGVSKQEISRR
                     ILKKAGELEASPELRDRFAETINRVNAATHGIRNDVGRGRSLEMFGRSAGRRNILALV
                     GSRHAPIYGENVNVRFADAPFSVAQVDMILRFLQTVRPVNVNRSESREGSVTRRVFLT
                     AGALGLAGAAITGMLAGRPAREYENPLMKHAELAGTYEDTAEGLVKLRVNSMPINQTP
                     YPGVPDFAWIKPDDIGYMMIADLLMMIHAREYPVGGLAKPELAKGRILDALKMMERLV
                     NDFELRVRVNGREIGTGVLPEVLVPRGDYFVPEQSDVPGQPGAKGIAYAFYNMGLTHE
                     RLKIIRDVFAGGIVEGIKDREISVLAAKLLARADYRPFIDAEKRIHAQVFKLSGTDEV
                     VRGPLIVDNQHTEARFILSIADLLGTPGKSEDERYAEGLESWNTMWFKWRSFRVDGKE
                     VLVADGDHVASLWTEGEGAQFFDPSEAAPLTWGRSFGNYGMILEDLKTRHGLTWTPAT
                     GLGTAQDPGVFSEFGIGNYPALPAVIVPAYQFVALTSGSAEAVAAASRFVEAAKKSGA
                     YVPGWGLPDAVDPVTAKAFNGKILYINQGLTLEALNYPVLREIVRKTAWGQRALREFR
                     AADAKRPAPRSELRKMTEKEQQAAAIEGLALIRGGKKGQDYSSDDEVLAIMDASGRLM
                     EKGLPKKEVHARHLEHMTGHIYFFDPAGNLIFQKRSPRKSQS"
BASE COUNT          757 a         1228 c         1100 g          828 t
ORIGIN      
        1 gcgactggga tttacgcggg ctccgtttct ggaagatcag atttcccgcg gggtcgaaga
       61 aataaatatg ccccgtcatg tgctcgagat gccgggcgtg gacctctttt tttggaaggc
      121 ctttttccat taaccggccc gaggcatcca tgatcgcgag cacctcgtca tcgctcgaat
      181 aatcctgccc cttcttcccg ccacggatca gagcgagccc ctcgatcgcc gcggcctgct
      241 gttctttttc ggtcatcttc cgcagttcgg agcgcggcgc gggacgcttc gcatccgccg
      301 cgcggaattc gcggagggcg cgctgacccc aggccgtttt tctgacaatt tcgcgcagga
      361 cgggataatt cagggcttcg agcgtgagcc cctgattgat gtaaagaatc ttgccgttga
      421 aggccttcgc ggtcacggga tcgaccgcat ccggaagccc ccagccggga acataggcgc
      481 cgcttttttt cgcggcctcc acgaaccggg acgcggcggc aacggcctcc gcggatcctg
      541 aagtgagcgc gacaaactgg tacgccggaa cgatcacggc cggcagcgcg ggataatttc
      601 cgatcccgaa ctcggagaaa acgccggggt cctgcgcggt gccgaggccc gtcgcgggcg
      661 tccaggtcag tccatgccgt gtcttgaggt cctccaggat catcccgtaa ttcccgaaac
      721 tccgtcccca ggtgagcggc gccgcttcgc tcggatcgaa gaactgcgcg ccttcccctt
      781 ccgtccagag cgacgcgaca tgatccccgt ccgccacaag cacctcttta ccgtccaccc
      841 tgaaggaacg ccacttgaac cacatcgtgt tccagctttc gaggccctcg gcataacgct
      901 cgtcctcgct tttccccggg gttccgagca gatccgcgat cgaaagaata aaacgcgctt
      961 cggtgtgctg gttatccacg ataagcggac cccgcacgac ttcatccgtt ccggagagtt
     1021 tgaaaacctg cgcgtgaatc cgtttttcgg cgtcaataaa aggccggtaa tcagcccgcg
     1081 cgagaagttt tgccgcgagg acgctgatct cgcggtcttt aattccctca acgatcccgc
     1141 ccgcgaacac gtcacggatg atcttgagcc gctcgtgcgt cagccccata ttgtaaaacg
     1201 cgtaggcgat ccctttggcg cccggctgac cggggacatc ggactgctcc ggcacaaagt
     1261 aatcaccccg cgggaccaaa acttcaggca gaaccccggt tccgatctcc cgaccgttca
     1321 ctctcaccct gagctcaaaa tcattcacca ggcgttccat catcttcaga gcatcaagga
     1381 tgcgtccctt ggcaagttcc ggtttcgcga gtccgccgac gggatactcc ctggcgtgaa
     1441 tcatcatcaa aagatcggcg atcatcatgt atccgatgtc atcgggcttg atccaggcga
     1501 aatccgggac cccgggataa ggagtctggt tgatcggcat ggagttgacg cgaagcttca
     1561 caagtccttc cgcggtgtct tcgtatgtcc cggccagttc ggcgtgtttc atcagcggat
     1621 tttcatattc ccgggcgggc cgtcccgcca gcatcccggt aatcgccgcg cccgccagtc
     1681 cgagcgcgcc tgccgtgaga aacacccgcc ttgtcacgga accttcccgc gattccgaac
     1741 ggttcacgtt caccggcctt accgtttgca agaacctcag aatcatgtcg acctgcgcga
     1801 cggagaaagg agcatccgca aaacggacgt tcacgttctc gccgtagatc ggcgcgtgcc
     1861 gggaccccac gagcgccagg atattccggc ggccggcgct tcggccaaac atttcaagcg
     1921 aacgcccgcg cccgacatcg ttcctgatcc cgtgcgtcgc ggcattgacg cgatttatcg
     1981 tttccgcaaa tctatcgcgc aattccggcg aggcttcgag ctcccccgct ttcttcagga
     2041 tgcgccttga gatctcctgc tttgaaacac cacaggtcat gcttcggtag gttaaaaacg
     2101 tttcccacgc ctgccgcgaa cttttccccg acattccggc ttcacccgcc agaaaaaaca
     2161 gcgtgtcgaa aagattcgcg gccatgactt cgtctttcgt cagtgaaagt cgtttttcga
     2221 tctccctgat aacttcggga tccgagggtg tgggaacggg atcgaccacc gggatcctga
     2281 gaaggtccga aagcttgatg agcacggtga catccgcgag ccgccccgac cgcgtcagcg
     2341 tgtttacccc gccttcaaga acgatctgcc atttttcagg cgattccgcg aggagcggcg
     2401 ccagctccag gattttctcg gcggctttgg gagccccgtg cggacccgcg atcagttcaa
     2461 gccggtagcc ttcccagctc tttgaaacat gattcaccgg ctgaacgatc atcggaggct
     2521 gaatgtgaaa acgcggataa ttttcgcggt aataggacgt caaagcatcc acggtcgaac
     2581 gaacccgcgc cgtttttttc gtttcggacc agaacagcgc tgcgcccgcg atcccggcaa
     2641 gcgcggcggc tcccgcgaaa acaaacatcc tccgtctcgg gacccgcgaa tcccgggctt
     2701 gccccggagg cgcgcgggaa ggctccgcgg caagatcaaa tatctgacgg agtttttcat
     2761 cactgacgga ctgaagcgcg gaaacagctt taaggtcctc cagggcgagc ttcgaacctg
     2821 ccggcagagc tttggcgaga tcgagaatct ggcgagccgt tgccatgcgc gcgtcctttt
     2881 ccttcagaac cggcttgagg gcttccgcga ttccctgcag tttcgcgtca cggtccagcg
     2941 tctgtcgaat gtcacttttt tccggcgcgc gggacttccc gcgtcgcgtt tgcgcgccca
     3001 gaacaaggcc gagcgtttcg gggtcagctt cggggttgaa tcttaaagcg tcacaaactc
     3061 tccggatcgc ttcgcggcca ttcgggagca cggtcacagg aatcggacgt gaggcgcgcc
     3121 ccaaagcggg cgggaccggt tgttcgaaag gcaacattcc ttccgtcatt aatttccggt
     3181 agatcttttc gtgacgcggg ctcggtttcg tgattccggg actcacgacc acatagccga
     3241 tccccctcgc ctcgaggatg cgtttgacgt tctccttgtg gaagcctcca tacaccaaaa
     3301 tcgccggctc actgccgggc aattttgagt gcggagtacg ggggtttttc cggaaatgcc
     3361 tgtccagcgc ttccgaaagc gcgccgtcgc gctccgcggc tatttcatag aacttcaccg
     3421 catcccggat gttccgctcc cattgtttcg aaaggacgag cggctttttc gcgtgtttat
     3481 ggatgaactc gccaagggat tgcgtatcaa aatacgccgg gagtccggga ccgggagatg
     3541 aggtctgccc cgggctcttg cctcccggct ccaggctgac gcgaagcgcc gcatattcct
     3601 cctgcgtcac catgagatcg ttcaggcgct caacgagacg aagggtccgg taatggccgt
     3661 agagttcccg gtcgacagcc tccggaagac aggcttcggc gatagaccgt tccagcctga
     3721 gaagctccgt gaacacttcg cgcgcgtcga tgtgtttcgc cttcccgatc acgacgggat
     3781 cgttcgattt aacggcgtcg aggatgaaac gaaggagtcc ctcgacgtgg agcgcagggc
     3841 gtctggtgtg gagcttttga ctatccgcca gagcgaccgt cctcgaaaga tacgtgaaca
     3901 gatccagttt ttt
//
LOCUS       DAOOBX010000045         3891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_26170_length_3891_cov_3.532325, whole genome shotgun sequence.
ACCESSION   DAOOBX010000045 DAOOBX010000000
VERSION     DAOOBX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26170_length_3891_cov_3.532325"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLY30_01120"
     CDS             <1..510
                     /locus_tag="PLY30_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64731.1"
                     /translation="PAGDVVGELFFDPEGQTMTEIQRYDFREISSDGGFGPIIFPKKV
                     TIVSPQTGKNTAIFLNNVKALDSATSYQFLLRIPKGTNEVFLREIDPKFTRMAIEAEK
                     IGTSSPGGETTTEENGPVAEMPATAVLSREGIPASEVAEKTEADDVISEEKAAEEAPQ
                     EADLAIQED"
     gene            complement(527..964)
                     /locus_tag="PLY30_01125"
     CDS             complement(527..964)
                     /locus_tag="PLY30_01125"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HPW64732.1"
                     /translation="MKKKKCKTFRLKEANALLPEIEKRLKRLQKKKEAYARVHDQLFV
                     HELVSAAERLNGLAENDDLEARIAGLEQAIEDLAKDVEAILEMGAILRDLERGRIDFL
                     ADLEGKPVFWSWELGEPSIGFYHHTNRNPRERIPIPARILKAP"
     gene            complement(1047..2405)
                     /locus_tag="PLY30_01130"
     CDS             complement(1047..2405)
                     /locus_tag="PLY30_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64733.1"
                     /translation="MTANLDYAFDEYLRSKSSEAVRAIQLFREKLKGRSLAFGRFTVP
                     AFYKAFFVTPKQQYLLKRAAVAFYQIINTTTRLYFEEPQLRARFHLSPEAEELVRIDP
                     GYAKNVVFGRFDTLLEGESLKILEFSCDAPSGASYTDQIETLFLSDPLAASFAGEHHL
                     VVSRRIQSILNALLAVYEEFGGYETPQIAIADWRHARMHAESEFMKSQFEARGYKTVI
                     ADPRDFQYKGGKLYHKGFRVHLVYRRVHFDEMVERLDELQDFLRAYRDKAVCVVNPLR
                     ARLSASKSLFSLLTDREFDRFFTDSENKMKQDHLPWTRGVADAEDFYGHRKVFLIDFL
                     QDEKETLVLKPPYSHGPEHVRIGRETTDSEWNTAVDRALKEQGWVIQEYVNVPAITVP
                     EVVNGKLDFAYKKYNFNMLVCGGKYAGGFVRLSDESVINVATGGGLMPAVWSDVAPET
                     NG"
     gene            complement(2466..3623)
                     /locus_tag="PLY30_01135"
     CDS             complement(2466..3623)
                     /locus_tag="PLY30_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HPW64734.1"
                     /translation="MPNSKKKEPSSASKLIKGLEGVVAAQTSLSDVDGENGRLLFRGI
                     PIGELVEKSHFEEVAYLLWYGKLPTGPELETFKDTLKAQRTLSKEAMAILKLLPTTTH
                     PMSALRTMCSLMGSLDPRAHIVNPEESQRKAYHLTGVFPTIIAAFFRLRSGKEPVVPD
                     MNLSHAANFLYMLNGEKPTPEMEKALDAYLILLADHGLNASTFAARVTTGTLSNLYSA
                     VTSAVGTLSGELHGAANQYAMEMVLEIGSADRAEAYVNKLLDEKKKVMGFGHRIYKTR
                     DPRAEAFREIAKDLCVKAGKTSWLEIAGVVEKVMMDRKKIPCNVDFFSAPVLYVLGFP
                     PDFFTTVFAASRVAGWSAHIIEQQLDNRLIRPLAEYVGPQDAYYIPAEDRK"
BASE COUNT          848 a         1120 c         1095 g          828 t
ORIGIN      
        1 cccgcggggg acgttgtcgg ggagctgttc tttgatcctg agggccaaac catgacggag
       61 atccagcgat atgatttcag ggaaatctcc tccgacggcg gcttcggacc gattatcttt
      121 ccgaagaagg tcacgatcgt ttcaccgcag accggcaaga ataccgccat cttcctcaac
      181 aacgtcaagg cactcgactc ggccacatcg tatcagttcc tgctgcgcat ccccaaaggc
      241 acgaacgagg tcttcctcag ggaaatagat ccgaaattca cgcggatggc gatcgaggcg
      301 gaaaaaatcg ggacttcctc gcctggcgga gagacgacaa cggaagagaa cggccccgtt
      361 gcggaaatgc cggcaaccgc cgtcctttcg agagaaggaa tcccggcgtc agaagtggcg
      421 gaaaagaccg aagccgatga tgtgatttcc gaagaaaaag ccgccgagga agcgccccag
      481 gaggcggatc tcgccatcca ggaggattga ggagcggttc ggaacgttac ggggccttga
      541 gaattctcgc ggggatcggg attctctcgc gggggttgcg gttcgtatgg tgatagaatc
      601 cgattgacgg ttcccccagt tcccaggacc agaaaacggg tttgccttcc aggtccgcca
      661 aaaaatcgat cctgcctctc tcaagatccc gaaggatggc tcccatctct aaaatggcct
      721 cgacatcttt cgcaaggtcc tcgatcgcct gttcgagccc ggcgatcctc gcttcgaggt
      781 catcgttctc ggcaaggcca ttcaaacgct cggccgccga caccagttcg tgcacgaaca
      841 gctggtcgtg aacgcgggca taggcttcct ttttcttttg gagacgcttc agacgttttt
      901 cgatctcggg aaggagggcg ttcgcttcct tcagcctgaa tgttttgcat tttttctttt
      961 tcatccgttt tatctcccgc ggaaaggtaa catacacccc atgatttcaa aaatcaaatt
     1021 gcaaaataga gcgcgccgcg cggacctcac ccgttcgttt cgggcgcgac atcagaccac
     1081 acggccggca tcagaccgcc gcccgtcgcg acattaatca cgctttcgtc gctcaggcgc
     1141 acaaaacctc cggcatattt cccgccacac accagcatat tgaaattgta tttcttgtag
     1201 gcgaaatcca gtttcccgtt cacgacctcc ggaacggtga tagccggcac gttcacgtac
     1261 tcctggatga cccatccctg ttccttgagc gcccggtcca ccgcggtatt ccattcgctg
     1321 tccgtcgtct cacgaccgat cctgacatgc tcgggtccgt ggctgtaggg cggtttcagc
     1381 acgagggttt ccttctcgtc ttgcagaaaa tcgatcagga agaccttccg gtgcccgtaa
     1441 aaatcctcag cgtcggcaac gccccgcgtc cacggcaagt gatcctgctt cattttgttc
     1501 tcgctgtccg tgaaaaagcg gtcgaactct cgatccgtaa ggagcgaaaa aagcgacttg
     1561 ctcgccgaaa gccgcgcccg caacggattc acgacgcaga ccgccttgtc gcggtaggcg
     1621 cgcaaaaaat cctggagttc gtcgagacgt tcgaccatct cgtcaaagtg gacccgccgg
     1681 tagacgagat ggacccggaa gcctttgtgg tacagcttcc cgcctttgta ttgaaagtca
     1741 cgagggtccg cgatcaccgt cttgtatccc cgcgcctcaa actgcgattt catgaattcg
     1801 ctttcggcgt gcattcgcgc gtgccgccag tccgcaatgg cgatttgcgg cgtttcgtaa
     1861 ccgccaaact cttcgtaaac tgccagaagt gcgttcagga tgctctggat acgacggctc
     1921 acaacaaggt ggtgttcccc ggcaaaggag gccgccagcg gatcggacag aaacaatgtc
     1981 tcgatctgat cggtgtagga agctcccgag ggcgcatcgc agctgaattc gaggatcttc
     2041 aggctttcac cttcgaggag cgtgtcgaac cttccgaaga caacgttctt ggcgtaaccg
     2101 ggatcgatcc ggaccagctc ttcggcttcc ggactcagat gaaagcgcgc ccgaagctgc
     2161 ggctcctcga aataaagccg tgtggtcgta ttgataatct ggtaaaacgc caccgccgcg
     2221 cgtttgagaa gatattgctg tttgggggtg acaaaaaagg ccttatagaa agcgggaacc
     2281 gtgaagcgac cgaaggccag ggaccggcct ttcagttttt cccggaacaa ctggatggca
     2341 cggacggcct cggaagactt cgaccgcaga tactcgtcaa acgcataatc aagattcgcc
     2401 gtcatggctc ggacctttcc cgaccgcggg accttttcct cgccgacatc atgcggtggc
     2461 tggatctact tgcggtcttc ggccggaatg taatacgcgt cctgaggacc gacgtactcg
     2521 gccaacggac ggatcaggcg gttatcaagc tgctgctcga tgatgtgcgc gctccagccc
     2581 gcgacgcggc tcgccgcgaa aacggtcgtg aagaaatccg gcgggaaacc gagcacataa
     2641 aggaccggcg cggagaaaaa atcgacgttg caggggatct tcttgcggtc catcatgacc
     2701 ttttccacga ccccggcgat ctcaagccag gaggtcttgc ccgccttgac gcatagatct
     2761 ttcgcgatct cgcggaaggc ctcggccctc ggatcgcggg tcttgtaaat acgatgcccg
     2821 aaacccatca ccttcttttt ctcgtcgaga agtttgttca cgtatgcctc cgcgcggtcc
     2881 gcggaaccga tttcaaggac catctccatc gcatattggt tcgccgcgcc gtgcagttcg
     2941 cccgagagcg tcccgaccgc ggaggtaacc gcggaataaa gattggaaag cgttccggtc
     3001 gtcacacgcg ccgcaaaggt cgaggcgttc agcccgtggt ccgcgagcag gatcagatat
     3061 gcgtcgaggg ccttctccat ttcaggggtc ggtttttcgc cgttcagcat gtaaaggaag
     3121 tttgccgcat gtgaaaggtt catgtcggga acaacgggtt ccttcccgga gcgaagacgg
     3181 aaaaaagccg cgatgatcgt cggaaacaca cccgtcagat ggtaagcctt ccgttgagac
     3241 tcttcgggat tcacaatgtg ggcccgggga tcgagcgatc ccatcagcga acacatcgtc
     3301 cggagcgcgc tcatcggatg cgtcgtcgtc gggaggagct tcaggatcgc catcgcctct
     3361 ttcgaaaggg ttcgctgcgc cttcagcgtg tccttgaagg tctcgagctc ggggcccgtg
     3421 ggaagtttcc cgtaccaaag gagataggcg acttcctcaa agtgggattt ctcgaccagc
     3481 tcgccgatcg gaataccgcg aaaaagcagg cggccgttct cgccatcgac atcgcttaag
     3541 gatgtctggg cggccacaac accttcgagg cccttgatca gtttggatgc gcttgacggt
     3601 tctttctttt ttgaattcgg catatcagga aatccctttc aaaatacccg cgcgactccg
     3661 cgcggaagac ccgactccgg ggggcacggc ccgtgtttcg gcgctcggct ccggctaatg
     3721 tttagggggc attatactca aaggcaggga aatgggaatt caaaatcagg atttgtgaca
     3781 aaaaagtcat tgggtcgcga tgcggaagga atacgggtca atctttcaga agctggcgca
     3841 acacgtactg caggatgccg ccgtgccggt agtattcaag ctcgacaggg g
//
LOCUS       DAOOBX010000046         3790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_27042_length_3790_cov_3.287818, whole genome shotgun sequence.
ACCESSION   DAOOBX010000046 DAOOBX010000000
VERSION     DAOOBX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27042_length_3790_cov_3.287818"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLY30_01140"
     CDS             <1..425
                     /locus_tag="PLY30_01140"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449220.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPW64735.1"
                     /translation="GASKKPVLLKRGLSATVKDLLMSAEYLLSRGNFKVVLGERGIRT
                     FETSTRNTLDLNAIPVIKKETHLPVLVDPSHGTGYRDFVTPMAMAGVAAGCDGLMIEV
                     HQCPEEAKSDGEQSLLPGQFEELMKKARAVAHAVGRDI"
     gene            508..1344
                     /locus_tag="PLY30_01145"
     CDS             508..1344
                     /locus_tag="PLY30_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPW64736.1"
                     /translation="MKFKKIVIVGLGLMGGSLAAACRKAFPKTLISGVTRNRRALARA
                     LRERWIDEGTGNVAFGASEADLIVLCTPVDVYLPILKEIDRVCGPGALVMDVGSVKGS
                     VHALVNAKKWRRISFVGAHPMVGSHKRGIEAGCPALYREGTVILTKDRKTCPGSFRKA
                     LRFWEKLVKKTVVLDPLTHDALVSEISHLPHAMAVCLVHAVSAKALPLAAGGFRDTTR
                     IAASDTSIWLPIFEANRRQVLRGVKKLERELEWFRRILRTSDTNRLRRYLDQAQKIRK
                     SL"
     gene            1545..3107
                     /gene="rpsA"
                     /locus_tag="PLY30_01150"
     CDS             1545..3107
                     /gene="rpsA"
                     /locus_tag="PLY30_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPW64737.1"
                     /translation="MQSQFAELYEKSFQSITPGDVVTGTVIQIRQKDVVVDIGYKSEG
                     ILPLDEFLDPAALAVGQEIEVLFEGFDDREGTALLSKRKADRQKTWNEILSNAEEGAI
                     VEGRIARKVRGGFMVDIGMEAFLPASLVDIRPVRNQDAFVGLQSKFLVVKINHKRKNV
                     VVSRKDLLEKSRQEARTEKLNELKVGQIVHGKVKNITDFGVFIDIGSLDGLLHITDMS
                     WGRISHPSDLVKIGDEIDVAVISIQKEKQKISLGLKQKSEDPWVTVERRYAIGNQVRG
                     KVVNILPYGAFVELEPGIEGLVHISELSWTRRVTHPSEVLSVGQELDVVILSLDTTAK
                     KISLGARQAQENPWDKVSDKYQVGSRIQGTVRNLTDYGAFVELEPGIEGLLHVSDLSW
                     THKVAKPSDMLKKGDTVEVMILNVDTDAKKISLGMKQLKDDPWNELTKGLMTGLEMQG
                     KITRVVNFGIFVELDNGLEGLIHVSELPDRNGQDLAKIYKVGDAIHCGILHVDHDGRK
                     IALTCKNEHVSS"
     gene            3119..>3790
                     /locus_tag="PLY30_01155"
     CDS             3119..>3790
                     /locus_tag="PLY30_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPW64738.1"
                     /translation="MQPSAGSHRRVVITGIGVVSSIGAGKKAFWESLSQGKSGVSLIS
                     SFDTSAFTTKFAGEIKDFDPAAHILPKKLKRMDRTTQLAVVASRMAVQDAGLDLQADG
                     HRERTGVIIGTALAGVGYILEQHDILRDRGPMRINPFTALASFPDACAGNVSIELGVT
                     GPSFSLATACSSASDACGYAFDAIRSGQLDFLIMGGAEATIFPGIMASFCVARALSTR
                     NDQPEK"
BASE COUNT          779 a         1068 c         1156 g          787 t
ORIGIN      
        1 gtggtgcttc caaaaaaccg gtccttctga agcgcgggtt gagcgccacc gtgaaggatc
       61 ttctcatgtc ggcggagtac ctcctttcca ggggaaattt caaggtcgtc ctcggggagc
      121 ggggaatccg gacgttcgag acatcgaccc gcaacacgct cgacctgaac gcgattccgg
      181 tgatcaaaaa agagacgcac cttcccgtcc tcgtggatcc ttcgcacggg accggttacc
      241 gtgatttcgt gacaccgatg gccatggcgg gtgtcgcggc gggatgcgac ggtctgatga
      301 tcgaggttca ccagtgccca gaagaggcaa aaagcgacgg cgagcagtcg ctcttgcccg
      361 ggcagttcga ggaattgatg aaaaaggccc gcgctgttgc tcatgcggtg ggaagggata
      421 tctgatacgg ggactcgtca agatgatttg atgatgagtt cccgggtgct agtcccgagt
      481 cctgcaagtc ggtagcgagg caaatcgatg aaattcaaaa aaatcgtgat tgtcgggctg
      541 ggtttgatgg gcggttcgct tgcggctgct tgccggaagg cgttccccaa gaccttgatc
      601 tcgggtgtga cccgcaatcg ccgggcgctt gcccgcgcgc tgagggagcg atggatcgat
      661 gaggggaccg ggaacgtggc gttcggggcc tcggaggccg atctcatcgt cctttgcact
      721 cctgtggatg tttatctgcc cattctgaaa gagatcgacc gcgtctgcgg gccgggcgcg
      781 cttgtgatgg atgtcgggag cgtcaaaggt tctgttcacg cccttgtgaa cgcgaaaaaa
      841 tggcggcgga tttccttcgt cggcgcccat ccgatggtgg gttctcacaa gcgggggatc
      901 gaagctgggt gccccgccct ctatcgagag gggaccgtaa tcctcacgaa agaccggaag
      961 acctgtcccg gaagtttccg gaaggcgctt cgattctggg aaaaacttgt gaaaaagaca
     1021 gtcgtgctcg atccgctgac ccatgacgcg cttgtttccg agatcagcca tctccctcat
     1081 gcgatggcgg tctgccttgt ccatgcggtc tccgcgaagg cgctaccgct tgcggccggg
     1141 ggattccgcg atacgacccg cattgcggct tccgacactt cgatctggct tcccatcttc
     1201 gaggccaaca ggcggcaggt tctgcggggt gttaaaaaac tggaacggga attagaatgg
     1261 ttccggcgta tcctgcgtac gtccgatacc aaccgcctca gacgatacct cgatcaggct
     1321 caaaagatcc gcaagagcct gtagctgtac gccgcgaagg ggctttagcc ctgtgccgtg
     1381 gggctcgcgg cgaaattaaa aatcatcctt cccaaacgaa atatctgtgt tatacttccg
     1441 cttgttcgcg tcctctccgt ggcgccagcc gtggaaaatt caaggcgcgg gcagcctcat
     1501 tccgccccag gcggaagagg gatccccaag gaggaatttc caccatgcaa tcccagttcg
     1561 cagagcttta cgagaagtct tttcagtcca ttacgccggg cgatgtcgtg accggaacgg
     1621 tcattcagat ccggcaaaaa gatgttgtcg ttgatatcgg atacaaatca gaaggcatcc
     1681 ttcccctgga tgagttcctg gaccccgcgg cccttgccgt cgggcaggag atagaagtgc
     1741 ttttcgaagg ttttgacgat cgtgaaggga cggcccttct ttccaaacgg aaggcggacc
     1801 gccagaagac ctggaacgag attctttcga acgccgaaga aggcgcgatt gtggaaggcc
     1861 gcatcgcccg caaggttcgc ggcggtttca tggtcgatat cggcatggaa gcgttcctcc
     1921 cggcctcgct cgtggatatc cgtcccgtcc gtaaccagga tgcgtttgtc ggtctccaga
     1981 gcaagttcct ggtcgtgaag atcaaccaca agcgcaagaa tgtggtcgtg tcgcgcaagg
     2041 acctgcttga gaagagtcgc caggaggcgc ggaccgagaa gctcaacgag ctcaaggtcg
     2101 gccagattgt ccacggcaag gtgaagaaca tcaccgattt cggcgtgttc atcgacatcg
     2161 gcagcctcga cggtcttctt catatcaccg atatgagctg gggccgtatt tcgcatcctt
     2221 ccgatctcgt gaagatcggc gatgagatcg acgtggccgt gatctcgatc cagaaggaga
     2281 agcagaagat atctctcggt ctcaaacaga aatccgagga cccgtgggtg acggtcgagc
     2341 gccgttacgc gatcggcaac caggtccgcg gcaaggtcgt caacatcctc ccgtacggcg
     2401 cgttcgtgga gcttgagccg ggcattgagg gacttgtcca tatcagcgag ctttcctgga
     2461 ccagacgcgt gacgcacccg agcgaagtgc tctcggtcgg tcaggaactg gatgtcgtga
     2521 tcctgagtct cgacacgacg gcgaaaaaga tctccctcgg cgcgcgccag gcgcaggaaa
     2581 atccgtggga caaggtcagc gacaagtatc aggtcggcag ccgcatccag ggcacggtcc
     2641 gcaatcttac cgattacggc gcgttcgtgg agcttgagcc cggcatcgaa ggcctccttc
     2701 atgtttccga tctttcgtgg acccacaagg tcgcgaagcc ttcggatatg ctgaagaagg
     2761 gcgatacggt cgaggtcatg atcctgaacg tcgacaccga cgcgaagaaa atctcgcttg
     2821 gcatgaaaca gctgaaggac gatccgtgga atgagctcac gaagggactc atgacgggcc
     2881 tcgagatgca gggaaagatc acccgcgtcg tgaacttcgg catcttcgtc gagcttgaca
     2941 acgggcttga gggactgatc cacgtttccg agcttccgga ccgcaacggt caggacctcg
     3001 cgaagatcta caaggtcggc gacgcgatcc actgcggtat cctgcatgtg gaccacgacg
     3061 gccgcaagat cgctctgacc tgcaagaacg agcacgtctc ctcgtaagac gggtgatcgt
     3121 gcagccttct gccgggagtc atcgccgagt agttattacc ggcatcggtg tggtttcatc
     3181 catcggtgcc ggtaagaagg cgttttggga gtccctttcg caaggcaaga gcggcgtgtc
     3241 gctcatttca tcgttcgaca cctccgcttt caccacaaag ttcgccggcg agatcaagga
     3301 tttcgatccg gccgcgcata tccttcccaa aaaactcaaa cgcatggacc gtacgaccca
     3361 gcttgcggtc gtcgcttcca ggatggcggt ccaggacgcg ggtctcgatc tccaggcgga
     3421 tggccaccgg gagcggaccg gcgtgatcat cgggacggcc cttgccggtg tcggttacat
     3481 tctcgaacag catgatattc tccgggaccg gggtccgatg cgcattaatc cgttcacggc
     3541 cctcgcgtcg ttcccggatg cctgtgcggg caatgtctcg atcgagctcg gcgtgacggg
     3601 tcccagtttt tcgcttgcga cggcctgttc ctcggcttcc gacgcctgcg gttacgcgtt
     3661 tgacgcgatc cggagcggcc aattggattt cctgatcatg ggtggcgccg aggcgacaat
     3721 cttcccgggc atcatggctt ctttttgcgt ggcacgcgcc ctttcgaccc gcaacgacca
     3781 gcccgaaaag
//
LOCUS       DAOOBX010000047         3786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_27066_length_3786_cov_3.608148, whole genome shotgun sequence.
ACCESSION   DAOOBX010000047 DAOOBX010000000
VERSION     DAOOBX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27066_length_3786_cov_3.608148"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLY30_01160"
     CDS             complement(<1..328)
                     /locus_tag="PLY30_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64739.1"
                     /translation="MKRFLALALLLMIALAPSAVATPWAEKEGYGEKTKGKLAFGLRN
                     TFLGWTEMFSEPKRYKFDLKKSEWEGFCMGISKFVFYTADGLIQLATFPIPVDFPDFG
                     EGAVPDL"
     gene            complement(325..660)
                     /locus_tag="PLY30_01165"
     CDS             complement(325..660)
                     /locus_tag="PLY30_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64740.1"
                     /translation="MKKILCAFLALSVLTSATPVFANFDNFQKSPWTEEKTYVDKSVG
                     KLAFGLTNITAGWTALFFETAKHGNIFAGIAKGVWRTGTNTVGGVLHAATFPVAVDIP
                     LPDGGVKFE"
     gene            909..2411
                     /locus_tag="PLY30_01170"
     CDS             909..2411
                     /locus_tag="PLY30_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPW64741.1"
                     /translation="MFKYPKVKASIPETRVKFNGKVLIIGCGGVSQCATPLILRHFDM
                     PAKNITIMDFVDNRHRVKDALKKGVRYVFDRVTRDNYRKLLAKYVGCGDMIIDLAWNI
                     DAWEILDWCRANNILYVNTSIEEWDPYRDDKRTDPTQYTLYYRHMKLRKLMKEKWGDE
                     KGATSIVDHGANPGLVSHFTKKALIDIAKKILKEKPRDPRAKNLERFLAGKNFAKLAQ
                     TTGVKVIHISERDTQVTDVPKRVNEFVNTWSIEGFYEEGVAPAELGWGTHEKKLPRDG
                     YEHRTGPKNQICLGTLGMNTFVRSWVPCGEINGMVIRHGEAFSISDRLTVWKNGKAVY
                     RPTVHYAYCPCDSAVLSMQELRMRQYKLHEDQRILSDDIIDGRDELGVLLMGHDFTSW
                     WCGSLLDIHTTRKLVAHQQATTLQVAVSVVAAALWMIDNPRKGFLLPDDIDHEFILKF
                     ATPYIVPFVSIPSDWTPLQNLNTKYLRFGAKAPKKDELWQFNSFLVDDRG"
     gene            2418..3599
                     /locus_tag="PLY30_01175"
     CDS             2418..3599
                     /locus_tag="PLY30_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HPW64742.1"
                     /translation="MKQPLLESGSISFLNKAVRPTESSGIENLIRQMLKKHKTPFLLI
                     RKSVLEKQFNRFRKALPEVTPYYAIKANPHPRIVKYFAKLGACFDVASAAEMKLVLKQ
                     GAKPSHIIFANTIKSKEDIVTARKRRVRLMTFDNEAELYKIAESFPGAHVILRIRVEN
                     RGSVVQLSLKFGAEPDQGFCLLRKAKSLGLVPKGVSFHVGSQSTNVENYLQALELTAG
                     IFREAKENGMPLSIVDIGGGFPIPHFENEIGINFERMAAQIRKQMKELFPKSTHFIAE
                     PGRFFAGPSGVLVTQVVGKAFRDNKNFYYINDGIYQDFSGMMFDHCKYEFKTLRRGQK
                     TMSVIAGPTCDSLDILTSNEEFPELFVGDVVYVKNIGAYSCASAVPNFNGFEPAKVIL
                     V"
BASE COUNT          870 a         1029 c         1109 g          778 t
ORIGIN      
        1 cgaggtccgg gaccgctccc tccccgaagt cggggaagtc gacgggaatg ggaaaggtcg
       61 cgagctggat cagaccgtcc gccgtgtaaa agacgaactt cgagattccc atgcagaacc
      121 cttcccattc gctcttcttg aggtcgaact tgtagcgctt cggctccgag aacatttcgg
      181 tccagccgag gaacgtgttg cggagaccga acgcaagctt ccctttggtc ttttcgccgt
      241 aaccttcctt ttcggcccat ggcgttgcga ccgcggaggg agcgagagcg atcatcaata
      301 gaagggcgag cgcgaggaag cgtttcattc gaatttcacg cctccgtcag gaagggggat
      361 atccaccgcg acggggaagg tagccgcatg gagaacgccg cccacggtgt tggtcccggt
      421 gcgccagaca ccctttgcga tgcccgcgaa gatgttgccg tgtttcgcgg tttcgaagaa
      481 gagggccgtc cagccggctg tgatgttcgt gagaccgaag gcgagcttgc cgaccgattt
      541 atcgacatag gtcttctctt cggtccaggg gctcttctga aaattgtcga aattcgcgaa
      601 gaccggcgtc gcggatgtca gaacagaaag ggcgaggaac gcgcaaagaa tttttttcat
      661 aaaggctcct ttttggacag cgggaagatt ctccacgtaa agcccgcttc cgtcaaggcg
      721 caatggcggc ttggggctga aggcctggct ctcggccttc ggcggggaat ttttaaaaaa
      781 atatgttttt tcggggttta gtggttgatg cagaaaacga tatgatgtaa tatccggttt
      841 ccgattcacg ggaaaaagtt ttttgtgacg gttattgtca aatttcaaac aatggagggt
      901 gagcgatcgt gttcaaatac cctaaggtca aagccagcat ccccgagacc cgcgtgaagt
      961 tcaacggaaa ggtcttgatt atcgggtgcg ggggtgtgtc gcaatgtgcg acaccgctga
     1021 tcctgcgtca cttcgacatg cccgcgaaga acatcacgat tatggacttc gtcgacaacc
     1081 gccaccgtgt aaaagacgcg ctgaagaaag gcgtccgcta cgtcttcgat cgcgtcacga
     1141 gggacaatta caggaagctt cttgcgaaat acgtcgggtg cggcgatatg atcatcgacc
     1201 tcgcctggaa tatcgacgca tgggagatcc tcgactggtg tcgcgcaaac aacatcctct
     1261 atgtcaacac ctcgatcgag gaatgggacc cttaccgtga cgacaagcga actgacccga
     1321 cccaatacac gctctactat cgccacatga agcttcgcaa gctcatgaag gagaagtggg
     1381 gcgacgagaa gggcgcgact tcgatcgtcg accacggcgc gaatcccggc cttgtgtccc
     1441 atttcacaaa gaaggccctg atcgatatcg cgaagaagat tctgaaggaa aagccgcggg
     1501 atccgcgcgc gaagaacctc gagcgctttc ttgccgggaa gaacttcgcg aagctcgcgc
     1561 agacaacagg cgtcaaggtt atccatatca gcgaacgaga cacgcaggtc acggacgttc
     1621 cgaaacgcgt caacgagttc gtgaacacct ggtcgatcga agggttctat gaggagggtg
     1681 tcgcgccggc cgaactcggc tgggggactc acgagaagaa acttcccagg gacggctatg
     1741 agcacaggac ggggccgaag aaccagattt gcctcggcac gcttggcatg aacacgtttg
     1801 ttcgttcgtg ggtgccctgt ggggagatca acggcatggt gatccgccac ggcgaggcgt
     1861 tttcgatctc cgaccgtctc acggtatgga agaacggcaa ggccgtgtac cgcccgaccg
     1921 tgcattacgc gtactgcccc tgcgattccg cggtcctttc gatgcaggag ctccggatgc
     1981 gccagtacaa gctgcacgag gaccagcgca tcctttcgga cgatatcatt gacggtcgcg
     2041 acgagctcgg tgtgctcctg atgggacatg atttcacgtc ctggtggtgc ggttcgcttc
     2101 tcgacatcca cacgacgcgc aaactggtcg cgcaccagca ggcgacgact ctccaggtgg
     2161 cggtctccgt ggtcgccgcg gcgctctgga tgatcgacaa tccgcgcaag ggttttctgc
     2221 tcccggatga tatcgatcac gagttcatcc tgaagttcgc gaccccttac atcgtcccgt
     2281 tcgtttcgat cccctcggac tggacgccgc ttcagaatct caacacgaag tacctgaggt
     2341 tcggggccaa ggcgccgaaa aaggacgagc tttggcagtt caactccttc ctcgtggacg
     2401 acaggggttg atccggaatg aaacagcccc ttcttgagag tgggagcatt tcgtttttaa
     2461 acaaggcggt caggccgacg gagtcctcgg gtatcgagaa tctgatccgg caaatgctga
     2521 aaaagcataa gacgcccttt ctcttgatcc gcaagagtgt cctcgagaag cagttcaacc
     2581 gctttcgaaa ggccctgccg gaagtgacgc cttattacgc gatcaaggcc aatccgcatc
     2641 cgcggatcgt caaatatttc gcgaagctcg gggcctgctt cgacgtcgcg agcgcggccg
     2701 agatgaaact cgtcctcaag cagggcgcga aaccgagcca catcatcttc gcgaatacga
     2761 tcaagtcgaa agaggatatc gtcaccgccc gcaagcgccg cgtccggctc atgaccttcg
     2821 acaacgaagc cgagctttac aagatcgcgg agagtttccc cggagcgcac gtgatcttga
     2881 ggatccgcgt cgagaaccgc ggctcggtcg tccagctgtc gcttaagttc ggcgcggaac
     2941 cggaccaggg gttttgtctc ttgcggaagg cgaaaagcct cgggctcgtg ccgaaaggcg
     3001 tgagctttca cgtcggttcg cagtcgacga acgtcgaaaa ttatttgcag gcgctcgagc
     3061 tcacggccgg gattttcagg gaggccaaag agaacggcat gccgctttcg atcgtcgata
     3121 tcggaggcgg gttcccgata cctcactttg aaaacgagat cggcatcaac tttgagcgga
     3181 tggccgcgca gatccgcaag cagatgaagg agctctttcc gaaatccact cattttatcg
     3241 ccgagccggg gcggtttttc gcgggtccct ccggcgtgct cgtgacgcag gtggtgggga
     3301 aggccttccg cgacaacaag aacttctact acatcaatga tggcatctac caggattttt
     3361 cagggatgat gttcgatcac tgcaagtacg agttcaagac cttgcgacgc gggcagaaga
     3421 ccatgagcgt gatcgcgggc ccgacctgcg attcgcttga tatcctcaca tcgaacgaag
     3481 agttccccga gttgttcgtc ggggatgttg tttacgtcaa gaatatcggc gcctactcct
     3541 gcgcgagcgc tgtccccaat ttcaacggct tcgaacctgc caaggtcatt ctcgtataaa
     3601 taaataatgg ggtcagtcac catttcaggg aaatggtgac tgaccccatc tctcaaggcg
     3661 caggccaagt ggattgcgat ccataaggac gccgaacgca aggccatccc aacggccgag
     3721 cagtggaaag aaatgaaaca acagaaggaa tgcccgtgga taaaagtgaa agggggcggc
     3781 atgaac
//
LOCUS       DAOOBX010000048         3759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_27322_length_3759_cov_3.291847, whole genome shotgun sequence.
ACCESSION   DAOOBX010000048 DAOOBX010000000
VERSION     DAOOBX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27322_length_3759_cov_3.291847"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2166)
                     /locus_tag="PLY30_01180"
     CDS             complement(<1..2166)
                     /locus_tag="PLY30_01180"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HPW64743.1"
                     /translation="MKAKTSATTGKKFASKYGYFTPDGREYVITRPDTPRPWVNVISN
                     GDYGTIETQTGSGFSWRDNSNLSRITRWDQDLIRDNWGKYIYLRDKDSGKYWSGTWKP
                     CMPKYDFFEVRHGQGYTVTTTGLNGIRFEKTIFVDVEDPVEVWKVVLTNETAKKRRLS
                     LFTYFDWCLGNAGDTHREFHKTFIEVWSDRKNGCLHGLKRAPLVPGFISTGMTEKPLQ
                     GFHSSFPKPVAFDGDKESFLGRYGEQSAPSAVVEGKCRNSEGKWGDASASLQVDVDLA
                     PKQSQTVIFTLGSTKTRDEAVRIIKKYQDPKNADRELARVRALWDSFVHATEVETPDE
                     AMNFMTNTWLKYQAISARLWGKCAYYQSSGGYGFRDQLQDCQIFFATRPELAKKQILL
                     HAEQQFPDGTVYHWWHHGTNIGAITQCSDDLLWLDFITLNYLDETDDRAILDIDVNFL
                     PDPKDPAKRVTSGSLYDHLLRAIDKALTRFSPRGLPLIGECDWNDGLSHVGIKWKGES
                     IWLGHFLYGILNRIAPIMKERGDKTKAKKYLHRAEALKEALNKDGWDGEWYLGATRDD
                     GRPLGSRSCETGKIFLNCQTWAVITGVSDKERSRVAMNSAAKWLYKKYGPLLLTPGYN
                     KTDPTIGYITRYAPSVRENGGLYTHAGTWAIQAEAMMRDGEKAYQVYKSFNPILRGLD
                     PDLYFAEPYVTPGNVDGPDSPNNGRGGWTWYTGSGAWYAV"
     gene            complement(2244..3533)
                     /locus_tag="PLY30_01185"
     CDS             complement(2244..3533)
                     /locus_tag="PLY30_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPW64744.1"
                     /translation="MKIKWVMRLFVYSVLLTSFVFSGCGAGPRNDGKTGVKVSFWGTP
                     EEIDIITQALSDWQKEHPEIKVVFEHTPYTGYTSKILTRIAGGAAPDIIATEVAYFVT
                     FASKGVLEDLTPYVDQDPSGFRKDDFFPQIIDRFTYDGKLMALPRDIAPFACVYYNKK
                     LFDAAGLPYPTDDWTWNDLLRIARDLTQKDESGRVTQYGFYGWAWQNFIYGNGGALVD
                     DVKNPTRTMLDDPKTIEGLQFYSDMSNLYGVMPTPMAFMNFGMGADRMFANDRVAMFL
                     SGIWETPLFRNYNISWDVVMFPKNAAGVRAFGTGGTGYCILKSSKHKKEAWEVIKALT
                     GPKGQELFAKRGLSQPARIEVAKGDAFANDPQPPANKKMLNEAVRHVVFSPLHPQWRE
                     IEEKILKPRLELVFNGKKRAEEVIKEVAAEINATLKR"
BASE COUNT          722 a         1072 c         1062 g          903 t
ORIGIN      
        1 cacggcatac caggcgccgg aaccggtgta ccaggtccag ccaccgcggc cattgttcgg
       61 agaatccggg ccatcaacgt tgccgggcgt cacatacggt tccgcgaaat aaaggtccgg
      121 gtcgagcccg cggaggatcg ggttgaaact tttgtagacc tgataggcct tttcgccgtc
      181 ccgcatcatc gcttcggcct gaatcgccca ggtgccggca tgcgtgtaaa gtccgccgtt
      241 ttcgcggacg gagggcgcgt agcgcgtaat gtagccgatc gtggggtcgg tcttgttgta
      301 gcccggcgtt aacagcagcg gcccgtattt cttgtaaagc cacttcgcgg ccgagttcat
      361 cgcgacccgg gaccgctctt tgtccgacac gcccgtgatc acggcccacg tctggcagtt
      421 caggaatatc tttcctgttt cgcaggagcg actgccaagc gggcgtccgt catcgcgcgt
      481 tgcgccgagg taccactcgc cgtcccagcc gtctttgttg agcgcttctt tcagcgcctc
      541 ggcgcggtga aggtattttt tggctttcgt cttgtcgccg cgctccttca tgatcggagc
      601 gatccggttc aggatgccat aaaggaaatg cccgagccag atcgactcgc ctttccactt
      661 gataccgacg tggctcagcc cgtcgttcca gtcgcactcg ccgatcagcg ggagcccgcg
      721 cggcgaaaaa cgcgtcagcg ccttatcgat cgcgcgaagc agatgatcgt aaagggagcc
      781 cgatgtgacc ctcttcgccg ggtccttggg atccgggaga aaattcacgt cgatatcgag
      841 gatcgcgcgg tcgtcggtct cgtcaagata gttcagcgtg ataaaatcga gccacaacag
      901 atcgtccgaa cactgcgtga tcgccccgat gttggtcccg tggtgccacc agtgatatac
      961 cgttccgtcc gggaactgct gttcggcgtg caggagaatc tgttttttcg ccagctcggg
     1021 acgtgttgcg aagaaaatct ggcagtcctg gagctggtcg cggaagccgt atccgccgct
     1081 cgattgatag taggcgcatt ttccccagag cctcgccgag atcgcctggt acttgagcca
     1141 tgtgttcgtc atgaagttca tcgcctcgtc cggcgtttcc acttcggtcg cgtgaacgaa
     1201 ggagtcccaa agcgcccgga ccctggcgag ttcccggtcc gcgttcttcg ggtcctgata
     1261 tttcttgatg atgcgcacgg cttcatcgcg ggtcttggtc gagccgagcg tgaagatcac
     1321 cgtttgggac tgcttcggag ccagatcgac atcgacctga agactcgcgg atgcatctcc
     1381 ccactttccc tcggagttgc ggcatttgcc ttccacgacc gcggaggggg cgctttgttc
     1441 gccgtatcgg ccgagaaatg attctttgtc gccgtcaaag gcaacgggct tcgggaacga
     1501 actgtgaaat ccctgcagcg gtttttcggt catgcccgtc gagatgaaac cgggaacgag
     1561 cggcgcacgc ttcaggccgt gcaggcatcc gttcttccgg tcgctccaga cttcgataaa
     1621 ggttttgtgg aactcgcgat gggtgtcccc ggcgttgcca agacaccagt caaaataagt
     1681 aaaaaggctg aggcggcgtt ttttggccgt ctcgttcgtc agaacaacct tccaaacctc
     1741 gaccggatcc tcgacatcga caaagattgt cttctcgaaa cggattccgt tcagcccggt
     1801 cgttgtcacg gtatatccct gtccgtgacg gacttcgaaa aagtcgtatt tcggcatgca
     1861 gggtttccat gttcccgacc agtatttacc gctgtccttg tcgcggagat agatgtactt
     1921 tccccagttg tcacggatga gatcctggtc ccagcgcgtg atgcgcgaaa ggttcgagtt
     1981 gtcgcgccag gaaaaaccgg agccggtctg tgtttcgatc gtcccgtaat cgccgttgga
     2041 gatcacgttg acccatggtc gcggcgtgtc gggccgcgtg attacgtact cccggccgtc
     2101 cggcgtaaag taaccgtatt tcgaagcgaa tttcttgccg gtcgtcgcgg aagttttcgc
     2161 tttcattttc gttttgattt tttttgtgcc ggtcttgctc atggtgggac gatcgctcct
     2221 ttgggatttc ggaagaaaac gtcttatctt ttcagcgtcg cattgatctc cgcggccact
     2281 tccttgataa cttcttccgc gcgtttcttg ccgttgaaaa caagctcgag cctgggcttg
     2341 aggatcttct cctcaatctc acgccattgc ggatggaggg ggctgaaaac cacatgccgg
     2401 accgcctcgt tcagcatttt cttgttcgcg ggaggttggg gatcgttggc aaaggcatcg
     2461 cctttggcga cctcgatgcg cgcgggctgg gaaagtcccc gtttcgcaaa aagctcctgc
     2521 ccctttggac cggtaagcgc cttgatcact tcccacgctt cttttttatg tttggaggat
     2581 ttcagaatgc agtagcccgt tccccccgtc ccgaacgcgc ggacgcccgc cgcgttcttc
     2641 gggaacatta cgacgtccca gctgatgttg taattgcgaa aaagcggcgt ctcccagatt
     2701 ccggaaagaa acatcgcgac gcggtcgttg gcaaacatcc ggtccgcccc cataccgaag
     2761 ttcatgaacg ccatcggagt cggcataacg ccgtaaagat tgctcatgtc agagtagaac
     2821 tgtaatccct cgatggtctt cggatcatcg agcatcgtcc gcgtcggatt cttgacgtcg
     2881 tcgaccaggg ccccgccgtt cccgtaaatg aagttctgcc acgcccatcc gtagaaacca
     2941 tactgcgtca cacggcccga ttcgtctttt tgggtcagat cgcgcgcgat ccgcagaagg
     3001 tcattccagg tccagtcgtc cgtcggataa ggaagccccg cggcatcgaa gagtttcttg
     3061 ttgtaataga cacacgcaaa cggcgcgatg tctctcggca gggccatcag ttttccgtcg
     3121 taggtgaagc ggtctatgat ctgcggaaaa aaatcgtcct tgcgaaaacc ggaagggtcc
     3181 tgatcgacat acggggtcag atcctcaaga actcccttgg aggcgaaggt cacgaagtag
     3241 gccacttcgg tcgcgatgat gtcgggcgcg gcgcctccgg cgatgcgcgt cagtatcttg
     3301 cttgtatagc cggtataggg ggtatgctca aaaacaacct tgatctcggg gtgttccttc
     3361 tgccagtcgg aaagcgcctg ggtgatgata tcgatctctt ccggagtccc ccagaagctg
     3421 accttgaccc cggtttttcc gtcgttcctg gggccggctc cgcacccgga aaaaacaaag
     3481 cttgttaaaa gaacggaata aacgaataaa cgcatcaccc attttatttt cattgtggtg
     3541 atccctttgc tgaagggtta tctgttttcg ttcgacgccc gcttgtgcac aggaaagtcc
     3601 caagaagcgc gtggattata gcacgcgacc tttcgaagtc aacggcttgc cttttgcttg
     3661 tccagccaaa atagaaatgt ccggttttca ggttgtaggt gagtcggcgt gtatcacatg
     3721 aatcccgagc tcctcggccg cgcgctgaaa ctggctctt
//
LOCUS       DAOOBX010000049         3685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_28009_length_3685_cov_2.831956, whole genome shotgun sequence.
ACCESSION   DAOOBX010000049 DAOOBX010000000
VERSION     DAOOBX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28009_length_3685_cov_2.831956"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /gene="sufB"
                     /locus_tag="PLY30_01190"
     CDS             complement(<1..1214)
                     /gene="sufB"
                     /locus_tag="PLY30_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPW64745.1"
                     /translation="MTDAGSFLDKEYPYGFVTPVDADAAPRGLNEDIIRFISKKKDEP
                     EFMLRWRLKAFAGWKKMKEPAWASLKYPPIDYQDIIYYSAPRSKKDGPKSLEDVDREI
                     LETYKKLGIPLEEQKRLAGVAVDAVFDSVSVATTFKEKLKEYGVIFCPFSEAVRAHPE
                     LIEKYLGSVVPVTDNYFAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINAAEIGQFE
                     RTLIVADDDACVSYLEGCTAPIRDKNQLHAAVVELVAHDRASIKYSTVQNWYAGDREG
                     RGGIYNFVTKRGLCRGQRSRISWTQVETGSAITWKYPGCILKGDDSVGEFYSVAVVNN
                     HQQADTGTRMIHLGKGTKSTIISKGISARFGKATYRGLVKMLPNAVNARNYTQCDSLL
                     IGDRCGANTFPY"
     gene            complement(1211..1675)
                     /locus_tag="PLY30_01195"
     CDS             complement(1211..1675)
                     /locus_tag="PLY30_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPW64746.1"
                     /translation="MRYTTKTEYGVICLAHMARKEWPGASTAKEIAEHEGFSVPFTEK
                     ILQGLRKAGITASQQGNKGGWTLTRQPSVITLREIVEALEGSTFETFCDLKRRDSIVC
                     RHFSICKLKPIWYKTKETLDGLYEKITLEQIAEDMMGAADGVPRGGGKRRGL"
     gene            complement(1753..2139)
                     /locus_tag="PLY30_01200"
     CDS             complement(1753..2139)
                     /locus_tag="PLY30_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64747.1"
                     /translation="MSFQRLIGEIKKIKFQEVRTSNETYFEAVIPKGDLQTLEKTLTR
                     HFGPPIKPAGVFAEGDANRYSRPYGGVRYEQTMYLLKAGGSLEFAFLWPWGCGTLVTL
                     KAISQESGGPVSDGGFGGFLRSLFGK"
     gene            complement(2136..2693)
                     /locus_tag="PLY30_01205"
     CDS             complement(2136..2693)
                     /locus_tag="PLY30_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64748.1"
                     /translation="MKRFWIFLALMALILPGAVGAEKFESPGNGFSIEVPKGWLRQPT
                     AYEGVLMTFWKEGTVATFHVTERDLEGAKTVSDLKWEDLFSPKFEEINIRQEAETMIN
                     GEKARYCIYKIRPGSFKQQVEGKGDFKYMNYLLVHNGRLYSVTFKDLESSFSIDYPGF
                     LSAVRSIRFTSGKTGIQPATTEERS"
     gene            complement(2704..>3685)
                     /locus_tag="PLY30_01210"
     CDS             complement(2704..>3685)
                     /locus_tag="PLY30_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPW64749.1"
                     /translation="KVPAKIRMKWGAAVRSTSVRAIPTMVKMLEEKGDVEFDQLQFTR
                     IKLWTPVGIYHSSSDGQWLYVQVPYVRGWVRAKDIAVFPSRDELKKYAGSDKFLVVTG
                     ESARVWKDSALTVFLQRPSMGTVIPRAMVSSKSDTAPYTVWMPYRRKDGSAGVAKAYV
                     SRKSDVRQAYPAYTQANIIRQAFKLLGQRYGWGGQYNGRDCSGFVHDVFLSMGVAFPR
                     DSKQQAIIGIQLGFFEPNEFDAEKRKTLNGARPGLTLIKMPQHQMIYLGRVGKQYYVI
                     HSTWAERTAYDPKKDEKNRINQVMVSDLELNGNSYIGSLFDRIVSINEID"
BASE COUNT          733 a         1130 c          979 g          843 t
ORIGIN      
        1 atatacggga aggtgtttgc gccgcaccgg tccccgatca gaagcgaatc gcactgcgtg
       61 tagttccgcg cgttcaccgc attcggcagc atcttcacaa gcccgcgata ggtggccttc
      121 ccgaaccgcg ccgaaatccc cttcgagata atggtgcttt tcgtgccctt cccgaggtgg
      181 atcatcctgg tgcccgtgtc cgcctgctgg tgattgttga caaccgcgac cgaatagaac
      241 tctccgaccg aatcgtcgcc cttcaggatg cagcccggat acttccacgt gatcgccgaa
      301 cccgtttcca cctgcgtcca ggaaatcctg gaacgctgtc cccgacagag accgcgtttc
      361 gtcacaaaat tatagatacc gccgcgccct tcacggtcac ccgcgtacca gttctggacc
      421 gtcgaatact tgatcgaggc ccggtcatgc gccacgagtt ccacgacggc cgcgtgcagc
      481 tggttcttgt cacggatcgg agcggtgcag ccttcgaggt agctgacgca ggcgtcatcg
      541 tcggccacaa tcaacgtccg ttcgaactgc ccaatctcgg ccgcgttgat ccggaaatac
      601 gtcgaaagtt ccatcgggca ccggaccccc ttcgggatgt aacagaagga accgtccgag
      661 aacaccgccg agttcagggc cgcgaaatag ttgtcggtca cggggacaac gcttcccaga
      721 tatttctcga taagctcggg atgggcgcgc accgcttcgg agaacgggca aaagatcacg
      781 ccgtattcct tcagcttctc cttgaacgtc gtcgcgaccg aaacgctgtc gaagaccgcg
      841 tcgacggcga cgcccgcgag acgtttctgt tcctcgagcg ggataccaag ttttttgtag
      901 gtctcgagaa tctccctgtc gacgtcctcg aggcttttcg ggccgtcctt tttcgaacgc
      961 ggcgcggaat aatagatgat gtcctgatag tcgatcggcg gatacttgag gctcgcccac
     1021 gccggctctt tcatcttctt ccatccggcg aacgccttga gacgccaccg caacatgaat
     1081 tccggttcgt cttttttctt cgaaatgaag cggatgatgt cttcgttgag cccgcgcggc
     1141 gcggcgtcgg cgtcgaccgg cgtcacgaat ccgtacgggt attccttatc gagaaaagaa
     1201 cccgcgtccg tcataaaccg cgtctctttc cgccgccacg gggaaccccg tccgcagccc
     1261 ccatcatgtc ctccgcgatc tgttcgagcg tgatcttttc ataaagaccg tccagggttt
     1321 ccttggtctt gtaccagata ggcttgagct tgcagatgga aaaatgccgg cacacgatgc
     1381 tgtcgcgccg cttcagatcg cagaacgtct cgaatgtgct tccttccagc gcttccacaa
     1441 tctcccgcaa agtgataacg gacggctggc gggtcagcgt ccatccccct ttattcccct
     1501 gctgggaggc ggtgattccc gccttgcgga gcccctgcag gatcttttcc gtaaaaggga
     1561 ccgagaagcc ctcgtgttcg gcgatctcct tggccgtact cgcgccgggc cattccttcc
     1621 tggccatgtg ggcgaggcag ataacaccgt attcggtttt ggtggtgtaa cgcatgatga
     1681 gctctccgga tgagggcgga attttatcaa accggactgt ttttgtccag attaaacccg
     1741 aaaaaaacgc tctcactttc caaagagaga gcgcaagaaa ccgccaaatc cgccgtcgga
     1801 aaccggcccg ccggactctt gactgatggc cttgagggtg acaagagtcc cgcaacccca
     1861 tggccacagg aatgcaaatt ccaggctccc cccggctttc aaaaggtaca tcgtctgctc
     1921 ataacggaca ccgccgtaag gacgggaata gcgattcgca tcaccttccg cgaaaacccc
     1981 cgcaggcttg atcggcggtc caaaatgcct ggtgagcgtc ttttcaagtg tctgcaaatc
     2041 ccccttcggg atcacggctt caaagtaggt ttcgttactt gtccggacct cctggaactt
     2101 gatcttcttt atttctccga tgagtctttg aaagctcatg atctttcctc cgtggtggcg
     2161 ggttggatcc cggtttttcc ggaggtgaac cggatcgacc gtacggccga gaggaacccc
     2221 gggtaatcga tgctgaatga cgattcaagg tccttgaaag tgaccgaata gagccggccg
     2281 ttatgcacaa gcaggtagtt catgtacttg aaatcccctt tgccttccac ctgctgcttg
     2341 aaggaacccg gcctgatctt gtagatgcag taacgggcct tttcgccgtt aatcatcgtt
     2401 tcggcttcct gccggatatt gatctcctcg aacttcgggc tgaacaggtc ttcccacttg
     2461 aggtccgaaa cggtcttcgc gccttcgagg tcccgctcgg tcacatggaa ggtcgcgacg
     2521 gtcccctctt tccagaacgt catgagcacg ccttcatacg ccgttggctg ccggagccag
     2581 cctttgggca cctcgatcga gaacccgttc ccgggggatt caaacttttc cgccccaacg
     2641 gctcccggca gtatcaatgc catcagggcc aggaaaatcc aaaatctctt catagcgctc
     2701 ctgtcagtca atctcattta ttgaaacgat ccggtcgaac agcgacccga tgtaggaatt
     2761 cccgttcagt tcgagatccg agaccattac ctggttgatc cggttctttt cgtctttttt
     2821 gggatcatag gccgtgcgct cggcccacgt cgaatgaatc acataatact gttttccgac
     2881 gcggccgaga tagatcatct gatgttgcgg catcttgatc agggtcagtc cgggccgcgc
     2941 cccattgagc gtttttcttt tttcggcgtc gaactcgttc ggctcgaaaa acccgagctg
     3001 gatcccgatg atcgcctgct gtttggaatc gcgcgggaac gcgaccccca tgctcagaaa
     3061 cacatcatgc acgaatcccg agcagtcgcg cccgttgtac tgtccgcccc acccatagcg
     3121 ttgtccgaga agcttgaagg cctgccggat aatgttcgcc tgcgtgtagg cgggataggc
     3181 ttgccggacg tcgcttttcc ggctgacgta ggccttggcg acacccgcgc tcccgtcttt
     3241 cctgcgatac ggcatccaga cggtgtaggg agccgtgtcg ctttttgagg agaccatcgc
     3301 ccgcggaatg actgtgccca tggaaggccg ctggagaaac acggtcagtg cgctgtcctt
     3361 ccagaccctt gcgctttctc ctgtcacgac caggaatttg tcggaaccgg cgtatttttt
     3421 aagttcatcc cgcgacggaa aaacagcgat atccttcgcc ctgacccacc cgcggacata
     3481 cggcacctgg acatacagcc actggccgtc gctggagctg tgataaatcc cgaccggggt
     3541 ccaaagcttg atccgcgtga actggagctg atcgaactcg acatcccctt tttcctcgag
     3601 catcttcacc atcgtgggaa tcgcgcggac cgaggtcgac cggacggccg cgccccactt
     3661 catcctgatc ttcgcgggaa ccttg
//
LOCUS       DAOOBX010000050         3662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_28261_length_3662_cov_3.581647, whole genome shotgun sequence.
ACCESSION   DAOOBX010000050 DAOOBX010000000
VERSION     DAOOBX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28261_length_3662_cov_3.581647"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PLY30_01215"
     CDS             complement(<1..961)
                     /locus_tag="PLY30_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPW64750.1"
                     /translation="MGKILGIDLGTTNSCMAIIEGTDPKIIANQEGNRTTPSVVAFTK
                     TGERLVGQTAKRQAVTNSENTVFSAKRFIGRRVEETDHEMQLVPFKVKTGKRGEAVFV
                     IPQEKRDFTPPEISAMVLQKLKVDAEMYLGEPVKQAVITVPAYFNDSQRQATKDAGQI
                     AGLEVLRIINEPTAAALAYGMDKKKDETIAVYDLGGGTFDISILEIGDGVFEVKATNG
                     DTHLGGDDFDQRVIDWMIAEFKKQEGIDLSKDRMAIQRLKEAAEKAKCELSTAQSTEI
                     NLPFVTADSSGPKHLLMTLTPAKLEQIVADLIERTAEPCMKAMK"
     gene            complement(1169..2527)
                     /locus_tag="PLY30_01220"
     CDS             complement(1169..2527)
                     /locus_tag="PLY30_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPW64751.1"
                     /translation="MFESILLVEDDSNSRKGLTQFLQALDYDVITAANGREGFDLFKE
                     ENPDLVIADIRMPEMDGVTLLEKIREEKPSAKVILLTAYGSVEDAVKAMKKGAFYYLT
                     KPVNLEELELLVKKTFSSRQLEEENRELKREIFSERYHEGTLIAESPKMKELLRTADK
                     IAASSASVLIEGESGTGKELIARRIHEMSPRSQNPFVAVHCAALTETLLSSELFGHEK
                     GAFTGAVERRQGRFERAHQGSLFLDEIGEISKDTQVKLLRVLQEGEFERVGGVKTIKV
                     DVRLIAATNKTLIDEVKKQSFREDLYYRINVIYLKMPPLRERKEDIPPLVNTFMRQYG
                     IQNGKKLRGIAPDALEALLRYDWPGNVRELKNIVERMIVLSAGEELTLDQVPDDIRGP
                     RPVAASFVSGNSAVTTLTDAEKELIRNALRETNGNKSKAAEKLGISRRTLYRKIEEYQ
                     IG"
     gene            complement(2520..>3662)
                     /locus_tag="PLY30_01225"
     CDS             complement(2520..>3662)
                     /locus_tag="PLY30_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW64752.1"
                     /translation="RVEKDTLYHKLRESVQEQILFRHICQNIEEGVLLLGPAGSVRFI
                     NRRAETWLALPSKPPVKTPVWELLSDATLSRFLKENLPGLDHSTVTDLRVLTPREIEL
                     RLTLSPYALGTEQGVLVLMNEISRSPEGEFETAMSRRMESLVRLAGGIAHEIGNPLNA
                     IMIHLELMKKRLKGLPSAKREEMESALSDIQQETKRLDRIIRNFLKATRKPPLRFRLA
                     DLGEILEDAVNFLKPELTAAKIRMKMTRDDSLPPFLMDRERLYYAFMNLIKNALEAMP
                     KGGDLKLSLIRRENCAVVSIQDTGCGIAPKELPRIFDIYYTNKQEGSGLGLMMVYDAV
                     TEHGGRIDVVSKLGKGSTFKIILPVREPQKQLPDYTQPKGGTHFHV"
BASE COUNT          646 a          992 c         1100 g          924 t
ORIGIN      
        1 ccttcatggc cttcatgcag ggttcggccg tgcgttcgat gaggtccgcg acgatctgct
       61 cgagcttcgc gggcgtgagc gtcatgagaa ggtgtttcgg tccggaacta tcggccgtga
      121 cgaacgggag attgatctcc gtgctttgtg cggtcgaaag ctcgcatttc gccttttccg
      181 cggcctcttt gaggcgctgg atggccatcc ggtccttgga gaggtcgatg ccttcctgtt
      241 ttttgaattc ggcgatcatc caatcaatga cgcgttggtc gaaatcgtcg ccgccgaggt
      301 gggtgtcacc gttggtggcc ttgacctcga acacgccgtc gccgatttca aggattgaga
      361 tatcgaacgt cccgccgccg aggtcgtaga cggcgatggt ctcgtctttt ttcttgtcca
      421 taccgtacgc gagcgccgcc gccgtgggtt cattgatgat gcggaggacc tcaagcccgg
      481 cgatctgtcc ggcgtctttc gtggcctgac gctgggagtc gttgaagtag gccgggaccg
      541 tgatgacggc ctgcttgacc ggttcgccga gatacatttc ggcgtcgacc ttgagcttct
      601 gcagaaccat ggccgagatt tccggcggcg tgaagtcgcg tttttcctgc gggatgacga
      661 agaccgcctc gccgcgcttg ccggttttta ctttgaacgg tacgagctgc atttcatgat
      721 ccgtttcttc gacccggcgt ccgatgaagc gcttggcgct gaagaccgtg ttctcggagt
      781 tcgtgacggc ctggcgcttt gcggtctgtc cgacgagtcg ctcccccgtt ttggtaaagg
      841 ccaccacgga cggagttgtc cggttccctt cctgatttgc gatgatcttc gggtcggttc
      901 cctcgatgat tgccatacaa gaattcgtgg tcccgaggtc gatgcctaag atttttccca
      961 tgtgacgatc tccttttttt gtttagggca tttttcaaac cgtgtatttt gaaatatcag
     1021 aatcaagtac catgccaata tggatattta ccgctaattg taacctgatt aaaacaaata
     1081 acttacaaca tcacaacctc cccccgggat cggattggct gaaccatgta acattaaggc
     1141 gcagccggat tgtgccaaat tggcaggatt aaccgatctg atattcttcg atttttcgat
     1201 agagcgtgcg ccgggaaatg ccaagtttct ccgcggcttt ggacttgttg ccgttcgttt
     1261 cccggagagc gttgcggatc agttcctttt cggcatccgt gagcgtggtg acagcgctat
     1321 ttcccgagac gaaggaggcc gcgaccgggc gcgggccacg gatatcgtcc gggacctgat
     1381 cgagggtgag ttcctctccg gcgctgagaa cgatcatcct ttcgacgatg ttcttgagct
     1441 cgcggacgtt tcccggccaa tcgtagcgga ggagggcttc aagagcgtcg ggcgctatcc
     1501 cccgcaattt tttgccgttc tggatgccgt actggcgcat gaaagtgttc acgagaggcg
     1561 gaatgtcctc tttgcgttct cgcagtggcg gcatcttcag atagatgacg ttgatacggt
     1621 aatagaggtc ctcgcggaaa ctctgttttt tgacctcgtc gatcagggtc ttgttggtcg
     1681 cggcgatcag ccggacgtcg accttgatgg ttttgactcc gccgacacgt tcgaattcgc
     1741 cttcctgaag aacgcggagg agtttgacct gtgtgtcctt cgagatttcg ccgatctcgt
     1801 cgaggaaaag gcttccctgg tgcgcgcgtt cgaaccggcc ctgccgacgt tcgacggctc
     1861 ccgtgaaggc ccctttttca tgtccgaaga gctcgcttga aagcagagtt tccgtcagcg
     1921 cggcgcagtg gaccgcgacg aaggggttct ggctgcgagg gctcatctcg tggatccgcc
     1981 gcgcgatgag ctcctttccg gtgccgcttt ccccttcaat gaggaccgag gcgctggagg
     2041 ccgcgatctt gtccgcggtc cggagaagct ccttcatttt cggggattcg gcgatgaggg
     2101 tcccttcgtg ataccgttcg ctgaaaatct cccgcttgag ttcccgattc tcctcctcga
     2161 gttgacggct gctgaaggtt tttttgacga ggagttcaag ttcctcgagg ttcacgggtt
     2221 tggtcagata ataaaaggcg ccctttttca tcgctttcac ggcatcctcg accgagccat
     2281 aggctgtcag gaggataacc tttgcggatg gtttctcctc gcggatcttt tcaaggagcg
     2341 ttaccccatc catttcgggc atgcggatgt ccgcgatcac aaggtcggga ttctcctctt
     2401 tgaaaagatc gaacccctca cggccgttcg cggcggtgat gacgtcgtag tcgagggcct
     2461 gaaggaattg ggtcaaccct ttgcgcgaat tcgaatcgtc ttcgacgaga aggatacttt
     2521 caaacatgga aatgcgtgcc tcctttgggc tgggtgtaat ccggaagctg tttttgcggt
     2581 tctcggaccg gcaaaatgat cttgaaagtg gagccttttc cgagcttgct cacgacatcg
     2641 atccggccgc cgtgttcggt gacggcgtcg tagaccatca tcagcccgag gccggagcct
     2701 tcctgcttgt tggtgtaata aatgtcaaaa atccggggca gttctttcgg agcgatcccg
     2761 cagcccgtat cctggatcga gacaaccgcg cagttttccc ggcggatcag tgacagcttg
     2821 aggtcgccgc ctttcggcat ggcctcgagc gcgttcttga tgaggttcat gaaggcgtaa
     2881 taaaggcgct cgcggtccat caggaacggc ggcaatgaat cgtcccgcgt cattttcatc
     2941 cggatttttg ccgcagtcag ctcgggtttg aggaaattca cggcgtcctc cagaatctcc
     3001 ccgaggtcgg cgagacggaa gcgaagcggc ggtttgcggg ttgccttgag gaagtttcgg
     3061 atgatccggt ccaggcgctt ggtctcctgc tggatgtcgc tcaaggcgct ttccatttcc
     3121 tccctcttgg ccgatgggag gcctttgagg cgttttttca tgagttcgag atggatcatg
     3181 atagcgttga gcggatttcc gatctcatgg gcgatcccgc ccgccaggcg cacgagactt
     3241 tccatgcgac gggacatcgc cgtctcaaac tccccttccg ggctcctgct gatctcattc
     3301 ataagtacga ggacgccctg ttcggtgccg agcgcgtatg gcgagagggt caggcgcagc
     3361 tcgatctcgc gcggcgtcag aacccgaagg tcggtgactg tgctgtggtc gagacctggc
     3421 aggttctctt tcaggaaccg gctgagcgtg gcgtccgaga ggagctccca aacgggtgtt
     3481 ttgacgggcg gtttggaagg aagcgcaagc caggtttcgg cgcggcggtt gatgaaccgc
     3541 acggaccccg cggggccgag cagcaggacg ccttcctcga tgttctggca gatgtgccgg
     3601 aacaggatct gttcctggac cgattccctg agtttatggt agagggtatc cttctcgacg
     3661 cg
//
LOCUS       DAOOBX010000051         3661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_28275_length_3661_cov_3.148364, whole genome shotgun sequence.
ACCESSION   DAOOBX010000051 DAOOBX010000000
VERSION     DAOOBX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28275_length_3661_cov_3.148364"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2431
                     /gene="nifJ"
                     /locus_tag="PLY30_01230"
     CDS             <1..2431
                     /gene="nifJ"
                     /locus_tag="PLY30_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPW64753.1"
                     /translation="GIFDFFASGKSKHGFTIGINDDVTHLSLPYDPSYSIESDKVVRA
                     MFYGLGADGTVGANKNSIKIIGENTDNYAQGFFVYDSKKSGAITVSHLRFGPEPIRSP
                     YLISSADFIACHQTVFLEKYDMLRNAKPGATFLLNVPWEADKVWENIPLEVQKAIIEK
                     KLKFYVIDAYKVAADSGMGMRINTIMQVCFFAISGVLPREQAIGEIKKSIQKTYGKKG
                     EEVVKRNLQAVDMTLAHLHEVKVPATADSKVRKLPPVTPDAPKFVKEVLGTIVEGRGD
                     DLPVSAFPVDGTYPTATTQYEKRNIALEIPVWDPEVCIQCGKCNIVCPHAVIRMKVYD
                     PKHLENAPQGFKSADAKMPAFKGQKFTIQVAPEDCTGCGVCVETCPAKSKTDPSKKAI
                     NMQPQIPLREAERPRWAHFLSLPEFDRTKLNPAMINQQQVMRPLFEFSGACGGCGETP
                     YVKLISQLFGDRAVIANATGCSSIYGGNMPTTPWAKDANGRGPAWANSLFEDNAEFGF
                     GFRMAIDKQKEFAGELLKKLETKIGADLVGALLGASQKTEAEIQAQRERVADLKKKLS
                     GLSSDPDVAQLLSLADTLVKKSVWIMGGDGWAYDIGFGGLDHVIASGKNVNILVLDTE
                     VYSNTGGQMSKSTPRSAVAKFAMGGKLTGKKNLGLIAMTYGNVYVASIAMGANDQQAL
                     KAILEAEAYDGPSIIIAYSHCIAHGINMTKGMNEQKAAVASGQWVLYRFDPSRIEKGE
                     NPLTLDSKTPTIPVEQFLMAENRFKMLAKSKPEHAKELFKLAQKDVDFRWKLYEYLAA
                     GKSQPELPKAA"
     gene            2476..>3661
                     /locus_tag="PLY30_01235"
     CDS             2476..>3661
                     /locus_tag="PLY30_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPW64754.1"
                     /translation="MSQDATANVSRKKDLKALFEPQTIAVVGSSRRPDAVGHAILRNL
                     LSTGYKGKLYPVNPKADEILGVKCCQTVADIPGLIDLAIFIIPSAGVPAELEACARKG
                     AKAAIIISAGFREIGAEGLKLENEVRRIADQYGISVLGPNCLGLINNDPAFSMNASFA
                     GTIPNPGNVAFVSQSGALGTALLDYAHGKNIGFSKFVSLGNKVGVTELEVLSYLKDDP
                     KTGVIIMYLEDIVKGYEFIEMAREITGEIAKTKPILVIKSGRTSEGAKAASSHTGSMM
                     GSDKVYDAVFTQSGVMRMDSALEMFGLATVFANQPLLKGNRIAIVTNAGGPGIMATDA
                     CVRAGLKMAEMSPLTVAKLKEKLPPTSNFSNPIDIIGDAQHDRYEHALRTVIAEPNVD
                     GVL"
BASE COUNT          817 a         1035 c         1123 g          686 t
ORIGIN      
        1 ggggatcttt gatttcttcg cttcgggcaa gtcaaaacac ggtttcacga tcggcatcaa
       61 cgacgacgtg acgcacctga gtctcccgta cgatccttcc tattcgatcg aatcggacaa
      121 ggtcgtgcgc gcgatgttct acggtctcgg cgcggacggg acggttgggg caaacaagaa
      181 ctccatcaag atcatcggtg aaaacacgga caattacgcg caggggttct ttgtctacga
      241 ctccaagaaa tccggcgcga tcacggtgtc gcatctccgc tttggtccgg aaccgattcg
      301 ctcgccgtac ctgatcagca gcgcggattt catcgcctgc caccagacgg tcttcctcga
      361 gaagtacgat atgctccgta acgccaaacc cggcgcgacg ttcctgctga acgtgccgtg
      421 ggaggcggac aaggtgtggg agaatatccc gctcgaggtc cagaaggcga tcatcgagaa
      481 aaaactgaag ttctacgtga tcgacgccta caaggtcgcg gcggacagcg ggatggggat
      541 gaggatcaac accatcatgc aggtctgctt cttcgcgata tccggcgttc tcccgcgcga
      601 gcaggctatc ggggagatca agaagtcgat ccagaagacc tacggcaaaa agggcgaaga
      661 ggttgtgaaa cggaatctgc aggctgtgga tatgacgctt gcgcaccttc atgaggtcaa
      721 ggttcccgcg acggcggaca gcaaggtccg taaactgccg cccgtgacgc cggatgcgcc
      781 gaagttcgtc aaggaagtcc tcggaacgat cgtcgaggga cgcggagacg accttcccgt
      841 gagcgcgttc ccggtggacg gaacgtatcc gaccgcgacg acgcagtacg aaaaacgcaa
      901 catcgcgctc gagattcccg tgtgggatcc cgaggtttgc atccagtgcg gaaaatgcaa
      961 catcgtctgt cctcacgcgg tcatccgcat gaaggtctac gatccgaaac acctcgagaa
     1021 cgcccctcag gggttcaagt cggcggacgc gaagatgcct gccttcaagg ggcagaagtt
     1081 cacgatccag gtggccccgg aggattgtac cggttgcggt gtctgtgtcg agacctgccc
     1141 ggccaagagc aaaacggacc cgtcgaagaa ggcgatcaat atgcagccgc agattcctct
     1201 ccgcgaggcc gaaaggccca ggtgggcgca ttttctttcc ctgccggagt tcgaccggac
     1261 gaaactgaat ccggcgatga tcaaccagca gcaggtcatg cgtcccttgt tcgagttttc
     1321 gggcgcttgc ggcggttgcg gcgaaacgcc gtacgtgaag ctcatttcac agcttttcgg
     1381 tgaccgcgcg gtaatcgcga acgccaccgg ctgttcgtcg atctacggcg gcaacatgcc
     1441 gacgaccccg tgggccaagg acgcgaacgg ccgcggtccc gcgtgggcga actcgctttt
     1501 tgaggataac gccgagttcg ggttcggttt ccgcatggcg atcgacaaac agaaggaatt
     1561 cgcgggcgaa cttctcaaga agctcgaaac gaagatcggc gcggatctgg tcggcgccct
     1621 gctcggcgct tcccagaaga ccgaggccga gatccaggcg cagcgcgaac gcgtggccga
     1681 tctcaagaaa aagctttcgg gcctttcgtc tgatcccgac gtcgcgcagc tcctttcgct
     1741 tgccgacacg ctcgtcaaaa agagcgtctg gatcatgggc ggtgacggat gggcctacga
     1801 catcggtttc ggcggtctcg atcacgtgat cgcatccggg aagaacgtga acatcctcgt
     1861 gctcgatacc gaggtctact cgaacaccgg cggtcagatg tcgaagtcga cgccgcgctc
     1921 cgcggttgcg aaattcgcga tgggcgggaa attgaccggg aagaagaatc tcggtttgat
     1981 cgcgatgacc tacggcaatg tctacgtcgc atcgatcgcg atgggcgcga acgaccagca
     2041 ggcgctgaag gcgatcctgg aggccgaagc ttatgacggt ccgtcgatca ttatcgcgta
     2101 cagccactgt atcgcgcacg gcattaacat gaccaaaggc atgaacgagc agaaggccgc
     2161 ggtcgcttcg gggcagtggg tgctctaccg gttcgatccc tccaggatcg agaaggggga
     2221 gaatccgctc acgctcgatt cgaagacgcc gacgataccc gtggagcagt tcctgatggc
     2281 ggaaaaccgc ttcaagatgc tcgcgaagag caagccggaa cacgcgaagg aacttttcaa
     2341 gcttgcccag aaggatgtgg atttccgctg gaagttatac gaatacctcg cggcgggaaa
     2401 gtcccagcct gaactcccga aggcggctta atttcaaccg agatcaacga tcattgaacc
     2461 gatgaaggaa ctccgatgag ccaagacgcg accgcaaatg tatcccgaaa aaaagatctc
     2521 aaggcactgt ttgagccgca gaccatcgcc gtggtcgggt cctcccgacg cccggacgct
     2581 gtgggacatg ccatcctgag gaatctttta agtacagggt acaaggggaa actctatccg
     2641 gtgaacccga aggcggacga gattcttggt gtcaaatgtt gccagacggt ggcggatatc
     2701 ccggggctga tcgatctggc cattttcatc atcccgagcg cgggtgttcc ggccgagctc
     2761 gaagcctgcg cgcggaaagg cgccaaggcc gcaatcatca tctctgcggg gttccgcgag
     2821 attggtgccg agggactcaa gcttgaaaat gaggtccgtc gcatcgccga ccagtacggg
     2881 atttcggtgc tcgggccgaa ctgcctcggg ctgatcaata atgatcccgc gttcagtatg
     2941 aacgcaagtt ttgcgggcac gatcccgaat cccggcaatg tcgctttcgt ttcccagagc
     3001 ggcgcgctcg gaacggccct gctcgattac gcgcacggga agaacatcgg gttctcgaag
     3061 ttcgtgagtc tcggcaacaa ggtcggggtg accgaactcg aggtcctgag ctatctcaag
     3121 gatgatccga agaccggtgt catcatcatg tatctcgagg atatcgtgaa gggctacgag
     3181 ttcatcgaaa tggcgcggga gatcaccggc gagatcgcga agaccaagcc gatccttgtt
     3241 atcaagtccg gccgcacgag cgaaggcgcc aaggcggcga gttcgcacac ggggtccatg
     3301 atgggctcgg acaaggtcta tgatgccgtg tttacgcagt cgggtgtgat gcgcatggat
     3361 tccgcgctcg agatgttcgg cctcgcgacc gtcttcgcga atcagccgct cctgaaaggg
     3421 aaccggatcg cgatcgtcac gaacgcgggc ggtcccggta tcatggcgac ggacgcctgc
     3481 gtgagggcgg gtctcaagat ggccgaaatg agccctctca cggtggccaa actgaaggaa
     3541 aaactgccgc caacctcgaa cttctccaat cccatcgaca taatcggtga cgctcagcac
     3601 gatcgctatg aacacgcgct ccggaccgtc attgccgagc cgaatgtcga tggcgtgctg
     3661 g
//
LOCUS       DAOOBX010000052         3614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_28729_length_3614_cov_2.811464, whole genome shotgun sequence.
ACCESSION   DAOOBX010000052 DAOOBX010000000
VERSION     DAOOBX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28729_length_3614_cov_2.811464"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLY30_01240"
     CDS             <1..397
                     /locus_tag="PLY30_01240"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HPW64755.1"
                     /translation="QLGASHKLTDRLTLRGGSWFYWTPVPKTHFTPSIPDANHLVLSL
                     GLGYKLSKYLVADLAYYNSFYFKRRINNDIAENAALLNSSVDGHYKTYSQGLTVSMTL
                     KWDDIFPRVTRAGDEESIGSSMSMNPAAR"
     gene            complement(473..1513)
                     /locus_tag="PLY30_01245"
     CDS             complement(473..1513)
                     /locus_tag="PLY30_01245"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPW64756.1"
                     /translation="MTEPIKVSVIGDGGWGTALALVNLRRGNEVLLWSVFPDYAKTLE
                     RKRVNERFLPGVRLPKDLRITSNLRAAVEFANVIVLAVPTQHLRSVLLRLKKLPYRGK
                     ILVNVAKGIENGTCLRPSEIVHKVLGKDVRLVVLSGPSHAEEVARKIPTMLVAASDDA
                     AAASYVQEHFQDPHFRIYVQKDVVGVELGGALKNVIAIAAGVCDGLKFGDNTKAGLMT
                     RGIYEIARLGVKFGASPDTFFGLSGIGDLFTTCASKHGRNLYVGRELVKGKKTGDILG
                     KMAMVAEGIGTSLSVHQLSRKYPDLQLPIMTEVYKMLFEGKSPADAVSSLLSRTPYDE
                     WKAFDLSAFRRK"
     gene            complement(1603..2028)
                     /locus_tag="PLY30_01250"
     CDS             complement(1603..2028)
                     /locus_tag="PLY30_01250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPW64757.1"
                     /translation="MEYFDHEYRVIYADTDRMGVTYYGHYFRFFEAARNEYFRALGLP
                     YTECEKKGLFLPVGEAHAKYIAPSTYDDELIIRTSVSHLGASSIKFQYQVIRKGTDKI
                     ITTGDTLHVFVDKTLRPCRIPDEIRQKVKVHTLLRSRKP"
     gene            complement(2048..3172)
                     /gene="dnaJ"
                     /locus_tag="PLY30_01255"
     CDS             complement(2048..3172)
                     /gene="dnaJ"
                     /locus_tag="PLY30_01255"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPW64758.1"
                     /translation="MKQKRDYYEVLGVSRDVTEDGLKKAYRKLALRYHPDRNPNSQEA
                     EEKFKEAAEAYAVLNDSDKRARYDQFGHSLGGGGFSGFENFQDSFGGFSDIFGDLFED
                     FFGGGGGGGRRSGARGRRGSDLEYPVTLTLEEVLSGKAMELEIPRRETCTECGGSGAE
                     KGSRKKVCADCGGRGEVRITQGFFSMRRTCPKCGGEGESIEKPCHVCRGEGRVRKVRK
                     LQVNVPAGIEDGSRLRVTGEGEAGQGAGGRGDLYIHVTVEKHKVFERQGHDLYAETLI
                     PYTVAVLGGKADAVTLTGKTELTIEPGTPSGKIVKVRHEGLPAVNMPDRRGDLYLRIQ
                     VEVPSKPSKAERELLEALAAERGDKVASKKGFFSHFNRVG"
     gene            complement(3247..>3614)
                     /locus_tag="PLY30_01260"
     CDS             complement(3247..>3614)
                     /locus_tag="PLY30_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPW64759.1"
                     /translation="EMLPVLDNFGRAVEHTTAGELAEADEEKLRQNFKNVATGIQMVQ
                     KQLTDILRNHGLTRLETVGEKFDPHRHEAVARVQEKGEEDVIVDELETGYMLHDRLLR
                     AAKVRVRTAPPPEKQDEIT"
BASE COUNT          694 a         1150 c         1007 g          763 t
ORIGIN      
        1 acagctcggg gcaagccaca agctcacgga caggctgacg ctccgcggcg gaagctggtt
       61 ctactggaca ccggtcccga aaacccattt cacaccaagt atccccgatg ccaatcacct
      121 ggtgctttct ctcggtctcg gctacaagct cagcaaatat cttgtcgccg acctcgccta
      181 ttacaactcg ttctatttca aacgccggat caataatgac atcgccgaga atgccgcgct
      241 cctcaactcc tccgtggacg ggcattacaa gacttattcg cagggcctta ccgtcagcat
      301 gacgctcaag tgggatgata tctttccccg cgtgacgcgc gcgggcgacg aggaatccat
      361 cggttcgtcc atgagcatga acccggccgc cagataaaaa ggtccgaaaa acaaaaaccc
      421 ggtaccggct ttggccggca ccgggttttt ttattttggg aacgatcagg tcttatttcc
      481 gcctgaacgc ggaaagatcg aacgccttcc actcgtcgta gggtgtgcgg gaaagcaggg
      541 acgaaacggc atccgcgggt gacttgcctt cgaacaacat cttgtaaacc tcggtcatga
      601 tcgggagctg gaggtccgga tacttgcggg aaagttgatg gacgctgagg gaggtcccga
      661 tgccttccgc gaccatcgcc attttcccga ggatgtcccc ggtcttcttc cccttgacga
      721 gttcgcgtcc gacataaagg ttccggccgt gctttgaggc acaggtcgtg aagagatcgc
      781 cgatccccga aagtccgaaa aaggtgtccg ggctcgcgcc aaacttcacg ccgagccggg
      841 cgatctcgta gatcccgcgc gtcataagac ccgccttggt gttatcaccg aacttcagac
      901 catcgcaaac acccgcggcg atcgcaatca cgttcttgag cgcgccgccg agctccacgc
      961 ccaccacgtc cttctggaca taaatacgga agtgcgggtc ctggaaatgc tcctggacat
     1021 aagacgccgc ggccgcatcg tcggacgccg caacgagcat ggtcggaatc ttgcgcgcga
     1081 cttcttcggc gtgggacgga cccgaaagga caacgagccg gacatctttc ccgaggacct
     1141 tgtggacgat ctcggagggc ctcagacagg tcccgttctc gatgcccttc gcgacattga
     1201 cgagaatctt tccgcgatac gggagcttct tcagcctgag aaggacgctc cgaaggtgct
     1261 gggtcgggac cgcgagaaca atcacattcg cgaactcgac ggctgccctg agattcgagg
     1321 tgatgcgaag gtccttcggc agtctcacac ccggaaggaa cctctcattc acgcgtttgc
     1381 gttcgagcgt cttcgcgtaa tcgggaaaga cggaccagag caggacttcg ttcccgcgcc
     1441 gcaggttcac aagcgcaagc gccgtccccc acccgccgtc acctataaca cttaccttga
     1501 tcggttcagt catgttattc accgctttaa acgttctcat tgccggtaca gcataacaaa
     1561 aatcgtgacc tgggacttgt tcccggggac agatccccgc attcagggtt tcctgctcct
     1621 caaaagcgta tgcactttca ctttctgccg gatctcgtcc gggatgcgac acggacgcag
     1681 cgtcttatcg acgaacacgt gcagcgtgtc accggttgtg atgatcttgt ccgtcccttt
     1741 ccggataacc tgatactgga acttgatcga gctcgcgcca aggtgactca cgctggtccg
     1801 gatgattaac tcatcgtcgt aggtcgaagg ggcgatatac ttggcgtgcg cctccccgac
     1861 cgggaggaag agtccctttt tctcgcattc ggtatagggc aggccgagcg cccggaaata
     1921 ttcgttgcgc gccgcctcga agaaacggaa gtagtggccg tagtaggtga cgcccatccg
     1981 gtccgtatcg gcgtaaatga cacggtattc gtgatcaaaa tattccattt ctgtctcccg
     2041 gaccgtttca gcccacacgg ttgaaatggc taaagaagcc tttcttcgac gcgaccttgt
     2101 cgccccgctc cgccgcgagg gcttcgagaa gttcccgctc ggccttcgac ggtttcgaag
     2161 gcacttcgac ctggatccgc aggtacaggt ccccgcgccg atccggcatg ttgaccgccg
     2221 gcagtccctc gtggcggact ttcacgatct ttcccgaagg cgtccccggc tcgatcgtga
     2281 gctccgtctt gccggtcagc gtcacggcat cggcctttcc gccgagcacc gcgaccgtgt
     2341 aagggatcag cgtttccgcg tagaggtcgt gcccctggcg ctcgaacacc ttgtgttttt
     2401 cgaccgtcac atggatgtag aggtctccgc ggccgcccgc gccctggccg gcctcgcctt
     2461 cccccgtcac ccgcaaccgg gaaccatcct cgatcccggc cggaacattc acctggagct
     2521 tgcggacctt gcggacgcgt ccttccccgc ggcagacgtg gcaaggtttc tcgatcgatt
     2581 ccccttcgcc gccacacttc gggcaggtcc gccgcatcga gaaaaatccc tgcgtgatcc
     2641 tgacctcgcc ccggccgccg caatcggcgc aaaccttctt ccttgaacct ttttccgcgc
     2701 cactcccgcc gcattccgtg caagtctcgc gcctcgggat ctcgagctcc atcgccttgc
     2761 ccgagagcac ctcttcgagc gtgagcgtca ccgggtattc gagatcggaa ccccggcgcc
     2821 cgcgcgcccc gctgcggcga ccgcccccgc ctccgccacc gaagaaatcc tcgaaaagat
     2881 cgccgaagat gtcgctgaag ccgccgaagg agtcctggaa gttctcgaaa cccgagaaac
     2941 cgccgccccc gaggctgtgc ccgaactggt cgtaacgggc gcgtttgtcg gaatcattca
     3001 gaacggcgta ggcctcggcc gcctccttga atttctcctc agcctcctga ctgttggggt
     3061 tgcggtccgg gtggtaccgg agggcgagct tccggtaggc tttcttcagt ccatcctccg
     3121 tcacgtcacg gctgacgccc aacacttcat agtaatcgcg cttttgtttc atagagtacg
     3181 gggtacggag tacggggtac ggagtaaacg gttttgagac tccatactcg gtacgccgta
     3241 ctgaatttag gtgatctcgt cctgtttttc gggaggaggg gcggtgcgga cccggacctt
     3301 cgcggcccgc aggagccggt cgtggagcat atacccggtc tccagctcgt cgacaatgac
     3361 gtcctcctct cccttttcct ggacgcgagc cacagcttca tggcggtgcg gatcgaactt
     3421 ctcgccgacc gtttcaagac gcgtgagacc gtggttccgg agaatatcgg tcagctgttt
     3481 ctggaccatc tggatcccgg tggcgacgtt cttgaaattc tggcgaagtt tttcctcatc
     3541 ggcctcggcg agctcaccgg cggtcgtatg ctcgacggca cggccaaagt tatcgagtac
     3601 ggggagcatc tcgc
//
LOCUS       DAOOBX010000053         3608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_28787_length_3608_cov_2.962848, whole genome shotgun sequence.
ACCESSION   DAOOBX010000053 DAOOBX010000000
VERSION     DAOOBX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28787_length_3608_cov_2.962848"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLY30_01265"
     CDS             complement(<1..484)
                     /locus_tag="PLY30_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HPW64760.1"
                     /translation="MFAKRIIPCLDVDHGRVVKGVKFVSLKDAGDPVECARAYDRQKA
                     DELVFLDITASHEKRRTMIAIAEKIAASIFIPFTVGGGINTVADIRELLRTGCDKVSI
                     NTAAVRNPRFVLEASKRFGSQCIVVAIDAKREGKDRWGVYTHGGRTPAGLDAVRWVKK
                     L"
     gene            complement(486..893)
                     /locus_tag="PLY30_01270"
     CDS             complement(486..893)
                     /locus_tag="PLY30_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64761.1"
                     /translation="MNELRELYEMILTQRTWSWAILGILDLLLFLTVRSLYFRPLVKR
                     AKTLNSKWYHEIKKAYLRRSLGGWLLFVVSLLLVVFTWQKTANLTTFTLCEAGLVTLI
                     VMMLLFAAMSHTTAFGTALIHVLKQLENNQMTL"
     gene            complement(890..1633)
                     /gene="hisA"
                     /locus_tag="PLY30_01275"
     CDS             complement(890..1633)
                     /gene="hisA"
                     /locus_tag="PLY30_01275"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPW64762.1"
                     /translation="MNLYPAIDLYQGKVVRLTQGDYNTCKVYSHDPAAIAKEWESAGA
                     RWIHVVDLEGAKTGVISNEASVSAICKAVKAQIEFGGGMRTADDIRKVLGLGVSRVIL
                     GTKALDPVFLDECVKTFGDKLAVGLDVRHGLVKTTGWTRDGNMTLEDVLALCDKANVH
                     TVIYTDINRDGVLDGPNFTKLTDVLARTRARVILSGGVARLTDIEKCGTITAGNFEGV
                     IIGKALYEKKFTLAEAFNVLPRNQKGVYP"
     gene            complement(1638..2243)
                     /gene="hisH"
                     /locus_tag="PLY30_01280"
     CDS             complement(1638..2243)
                     /gene="hisH"
                     /locus_tag="PLY30_01280"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPW64763.1"
                     /translation="MITIIDYGMGNLRSVSKAVEHLGGRCRFAGKGSDLRNAKRIILP
                     GVGAFGNAMQELKKRCLILPLMEALEKGTKLLGVCLGLQLFFESSEEAPGIKGLGLFP
                     GKVLRFRSKKAKIPHMGWNDLVAVKKHPLLNGIRAGDYFYFLHSYYGKPGIKDLTLAS
                     CRYAGRDFAAVVGNSRIAATQFHPEKSQDAGLRILSNFLKW"
     gene            complement(2319..2915)
                     /gene="hisB"
                     /locus_tag="PLY30_01285"
     CDS             complement(2319..2915)
                     /gene="hisB"
                     /locus_tag="PLY30_01285"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPW64764.1"
                     /translation="MKKRSAVIKRKTSETDIAVALTIDGRGRSSVNTEIPFLDHMLTL
                     LAKHGLFDLTLKARGDLEVDIHHTNEDIGIALGQAFSKALGDKKGIRRYGSFAVPMDE
                     SLVRITLDISCRPSLFVKKFPGVRLSRQPKYTLDDAIDFLRSFCQHAGINMHIGVLAG
                     EDTHHIIEGIFKALAKTLDMAVSVDPRVKGVPSTKGKL"
     gene            complement(2912..>3608)
                     /locus_tag="PLY30_01290"
     CDS             complement(2912..>3608)
                     /locus_tag="PLY30_01290"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPW64765.1"
                     /translation="TRTVPAVDKIVGPGNAYVTEAKRQVYGFVDIDMVAGPSEVAIIA
                     DHTANADFVTCDLLAQTEHHGGIGYLITPSRKLVEIMKGRIDTGIIIQVKSVQEACEV
                     ANEIAAEHLEIMTEEPEKVQKSIRHAGAIFIGPYSPTVIGDYIAGPSHVLPTGGTARY
                     FSPLSASTFIKSSQIIRYTKEALVASKEHVQKLTEMEGLYLHRISLEARLASPKTTPA
                     VKTNPETGKGPAS"
BASE COUNT          687 a         1001 c         1113 g          807 t
ORIGIN      
        1 cgagcttttt tacccagcga accgcgtcga ggccggctgg ggtcctgccg ccgtgcgtgt
       61 agacgcccca ccggtccttc ccctcgcgct tcgcgtcgat cgcgaccacg atgcactggc
      121 ttccgaaccg tttgctcgcc tcgagcacga accgcggatt ccgtaccgcg gcggtgttga
      181 tcgagacctt gtcgcatccg gtgcggagca gttcgcggat gtccgcgacc gtgttgatgc
      241 cgccgcccac cgtgaacggg atgaagatcg acgcggcgat cttttcggcg atagcgatca
      301 tcgtgcggcg tttctcatgg gaggctgtga tgtcgaggaa cacgagctcg tccgccttct
      361 ggcggtcata ggcccgcgcg cattcgaccg gatcgcccgc gtctttcagg gagacgaact
      421 tgaccccttt gacgacacgg ccatggtcga cgtcgagaca tggaatgatg cgttttgcga
      481 acatatcaga gggtcatctg gttgttttca agctgtttga ggacgtggat cagggcggtt
      541 ccgaacgcgg tggtatgaga cattgccgcg aaaaggagca tcatcacgat cagggtaacg
      601 agacccgcct cacagagcgt gaaggtcgtg aggtttgcgg tcttctgcca cgtaaagacc
      661 acgaggagga gcgagacgac gaaaagaagc catcctccga gggaacgccg gaggtaggcc
      721 tttttgatct cgtggtacca cttggagttc agggtcttgg cccgtttgac gagggggcgg
      781 aaatagaggc ttcggactgt caggaataaa aggaggtcga gaattcccag aatagcccag
      841 gaccaggtgc gctgcgtcag gatcatttcg tagagttccc gtaactcgtt catgggtata
      901 cccctttctg gtttcttggg agaacattga aggcttccgc gagcgtgaat tttttttcgt
      961 aaagggcctt gccgatgatg acgccctcga agttcccggc cgtgatcgtg ccgcatttct
     1021 cgatatcggt cagccgtgcg acaccgcccg agaggataac acgcgcccgc gtgcgggcga
     1081 gcacgtcggt aagctttgtg aaattcggtc cgtcaagcac gccgtccctg ttgatatccg
     1141 tgtaaatgac ggtgtggacg tttgctttgt cgcaaagcgc caggacgtct tcgagcgtca
     1201 tgttcccgtc ccgcgtccat ccggtcgtct tgacgaggcc gtgccggaca tcgagtccga
     1261 cggcgagctt gtccccgaac gtcttgacgc actcgtccaa gaaaaccggg tcgagggcct
     1321 ttgtcccgag aatgacgcgg gagacgccga ggccgaggac cttacggatg tcatcggcgg
     1381 tgcgcattcc cccgccgaac tcgatttggg ccttcacggc tttgcagatc gccgagacgg
     1441 atgcttcgtt ggaaatgacc ccggtcttcg cgccttccag atccacgaca tggatccatc
     1501 gggctccggc cgattcccac tcctttgcta tggcggccgg atcatgggaa tagaccttgc
     1561 atgtattgta atcgccctgg gtcaggcgga cgaccttgcc ttggtagaga tcgattgcgg
     1621 gatacagatt catggggtca ccatttgagg aagttggaaa gaatgcgcaa tcccgcatcc
     1681 tggctcttct cagggtggaa ctgggttgca gcgatccggg agtttcccac gacggcggcg
     1741 aaatcccgcc ccgcgtaacg gcaactggcg agggtcaggt cctttattcc cggttttccg
     1801 tagtaggaat gtaaaaagta gaagtagtcg cctgcgcgga tgccattgag gagagggtgt
     1861 tttttgaccg ccacgagatc gttccagccc atgtgcggga tcttggcttt tttcgaccgg
     1921 aaccggagta ccttgccggg gaaaagcccg agacctttga ttccgggcgc ctcttcgctg
     1981 gattcaaaga aaagctgtag accgaggcag acacccagaa gtttcgtgcc tttctcgaga
     2041 gcctccataa gcgggagaat gagacagcgt tttttaagct cctgcatcgc gttcccgaag
     2101 gcgccgacgc cgggcaggat aatccttttc gcgttcctga gatcgcttcc cttgcccgcg
     2161 aagcggcatc gaccgccgag gtgttcgacg gccttggaga cgctccggag attgcccatt
     2221 ccgtagtcga tgatcgtaat catgcgcgtt gagagccctg aggcgggaac cgggagccga
     2281 agaccttgac tccggactcc gttctccaaa ctccgggctt aaagttttcc tttggttgac
     2341 ggaacaccct tgacccgagg gtcgacgcta acggccatat cgagcgtttt ggcgagcgcc
     2401 ttgaaaatac cctcgatgat gtgatgtgtg tcttcgccgg cgagtacgcc gatgtgcatg
     2461 ttaataccgg catgctggca aaaggaccgg agaaaatcga tcgcatcgtc cagcgtgtat
     2521 ttcggctggc gggaaagccg gacgcccggg aattttttga cgaagaggga cgggcggcac
     2581 gaaatatcaa gcgtgatccg gacgagggac tcatccatcg gcacggcgaa cgagccgtat
     2641 cgccggatcc cttttttatc accgagtgcc ttcgagaatg cctggccgag cgcgatcccg
     2701 atatcctcgt tcgtgtggtg gatatcgacc tccaggtcgc cgcgggcctt gagcgtcaga
     2761 tcgaagaggc cgtgtttggc aaggagcgtc agcatgtggt cgaggaacgg aatctccgta
     2821 tttacggaag aacgaccgcg tccgtcgatc gtgagggcga cggcgatatc cgtttcggag
     2881 gttttgcgtt tgatgacagc cgaacgtttc ttcatgaggc cggtcccttt cccgtttccg
     2941 gattggtttt tacggcaggg gtcgtcttcg gcgaagcgag gcgggcctcg agcgagattc
     3001 tgtgcaggta gaggccttcc atttcagtca atttctggac atgttccttc gacgccacga
     3061 gagcctcctt ggtatagcgg atgatttgcg agctcttgat gaatgtcgat gcgctgagcg
     3121 gcgaaaaata acgggccgtc ccgcccgtgg gcaggacgtg gctcggtccg gctatgtaat
     3181 ccccgatgac ggtcggcgaa taagggccga tgaagatcgc tcccgcgtga cggatcgatt
     3241 tttggacctt ttccggttcc tcagtcatga tctcgaggtg ctcggccgcg atctcgttgg
     3301 cgacctcgca ggcctcctgg acggatttga cctggatgat gatgccggtg tcgatccttc
     3361 ctttcatgat ctcgacgagc ttcctggacg gcgtgatcag ataaccgata ccgccgtgat
     3421 gttctgtctg ggccagcagg tcgcaggtca cgaaatccgc gttggcggtg tggtccgcga
     3481 tgatcgcgac ctcgctcggt ccggcgacca tgtcgatgtc aacgaacccg tagacctgac
     3541 gcttcgcttc cgtgacgtag gcattccccg gtccgacgat cttgtccacg gccggaaccg
     3601 tccgggtc
//
LOCUS       DAOOBX010000054         3584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_29021_length_3584_cov_3.007934, whole genome shotgun sequence.
ACCESSION   DAOOBX010000054 DAOOBX010000000
VERSION     DAOOBX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29021_length_3584_cov_3.007934"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLY30_01295"
     CDS             complement(<1..753)
                     /locus_tag="PLY30_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPW64766.1"
                     /translation="MIVGFLRRLSIVFGKEMAAYFNSAIAYIFLFVFALLNCGLFMTQ
                     FFMMGRADMRSFFYGLPVLLCVFLPAVSMRLWSEEKRGNTQELLLTFPLATNELVLGK
                     FLASFCFYLIALASTASIPVMLVVLGQPDLGAILGGYLGAAVLGAFFLAAGILVSGFC
                     RDQITAFILSMMLCFALYLIGTDFAATSLDGWVAGLGSFLRRTLGAADHFTTFSKGVV
                     DNRGLAYFAVGTAVCLILNGFWIEGRMRPKAAS"
     gene            complement(750..1478)
                     /locus_tag="PLY30_01300"
     CDS             complement(750..1478)
                     /locus_tag="PLY30_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010035481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW64767.1"
                     /translation="MIDVKNLTMRYGHVTALDGVSFRANPGEILGLLGPNGAGKSTAM
                     RILTTYLYPTSGTASVDGLDILKDPVGVRRRIGYLPETVPLYGFMNVEEYLRFVGAAR
                     GLSGAQLRKRLGWVMDACQLKKVWKQPTAELSKGYGQRVGLAQSLIHDPGVLILDEPT
                     SGLDPIQILGIRKLVKSLSKDKTIVFSTHILQEVEALADRIVIINEGRLVACGTREEL
                     SAKAGKPGASLEEVFISLLAPEGV"
     gene            complement(1577..2260)
                     /locus_tag="PLY30_01305"
     CDS             complement(1577..2260)
                     /locus_tag="PLY30_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HPW64768.1"
                     /translation="MDPNHNSQPYSGAAALSSFIQRVYFWMAMGLGLTGLAAFATVLN
                     AGFLMFLVKGGMWLFFIVELFLVFWLSANIRRLSASAATMLFLVYSALNGVTLSSIFL
                     VYTATSISVTFFICAAMFAGVSLYGWVTKQDLTSVGSFCAMAIWGVLIASLVGMFFRA
                     PAFHWILSYVGVAVFIGLTAYDTQKLKWIHQNAQGAADQLAILGALALYLDFVNMFLF
                     LLRIFGRRR"
     gene            complement(2382..2843)
                     /locus_tag="PLY30_01310"
     CDS             complement(2382..2843)
                     /locus_tag="PLY30_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HPW64769.1"
                     /translation="MEKITIYQKPSCSACKEVCQVLKDAGAEIESVDYYVTRIPKAKL
                     CELARKMGVPLRELLRTKEQIYEDLRLGEKNLTDAQYLDLIEIYPDLLQRPIVEQGDR
                     AILARPVRKIREFLVSAVSSADGQAPAASVTEGQSPAADETTGTTDSLGQP"
     gene            complement(2923..3438)
                     /locus_tag="PLY30_01315"
     CDS             complement(2923..3438)
                     /locus_tag="PLY30_01315"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPW64770.1"
                     /translation="MTQEILHKGKFLDLVREGNWEYVRRTNCDGIVVIVPRTEDGRVV
                     FVEQHRKPVKASVIEWPAGLVNDKTSHDPESMESAAKRELLEETGYEAGKLERVAEGP
                     VSSGLSAEIITFYLATGIVKKHKGGGDETESITVHEVPLAEAESWLLAMEKSGRLVDP
                     KVYVGLYFLKS"
BASE COUNT          794 a         1082 c         1034 g          674 t
ORIGIN      
        1 cgaagccgcc ttcggccgca tccgtccctc gatccagaaa ccgttcagga tgaggcagac
       61 cgcggtcccg accgcgaaat aagcgagacc ccggttgtca acgacgcctt tcgaaaatgt
      121 cgtgaaatga tccgccgcgc cgagcgtacg ccggagaaaa ctgccgagcc cggcgaccca
      181 cccgtccagc gaggtcgccg cgaaatccgt cccgatcagg tagagggcga agcagagcat
      241 catgctgagg atgaaggccg tgatctggtc gcggcagaat cccgagacga gaatccccgc
      301 cgcgaggaag aaggccccga ggaccgcggc gccgagataa ccgccgagga tggcaccgag
      361 gtcgggctgg ccgagcacga cgagcatcac ggggatcgac gcggtcgacg cgagcgcgat
      421 caggtagaaa cagaaacttg ccagaaattt ccccagcacg agctcgttcg tggctagcgg
      481 gaaggtcagg agaagctcct gcgtattccc ccgtttctcc tccgaccaga gccgcatgga
      541 gaccgccgga agaaaaacac agagcaggac aggcaggccg tagaagaacg aacgcatgtc
      601 cgcccggccc atcatgaaga actgggtcat gaagagcccg cagttcagaa gagcgaaaac
      661 aaaaagaaag atatacgcga tggcggaatt gaaataggcc gccatttcct tgccgaacac
      721 gatggaaagc cgccgcagga atcccacgat cacactccct ccggggcaag aaggctgatg
      781 aaaacctctt cgagagatgc gccgggtttc cccgctttcg cggaaagttc ctcccgcgtc
      841 ccgcaagcga caagccggcc ttcattgatg atgacgatcc ggtcggcaag cgcttcgacc
      901 tcctgaagga tgtgcgtcga gaagacgatc gtcttgtcct tcgagaggct tttgacgagc
      961 ttgcggattc cgagaatctg gatcgggtcc agccccgagg tcggttcgtc gaggatcagg
     1021 accccggggt catggatcag cgactgtgcg aggccgacac gttgcccgta acctttggag
     1081 agttcggccg tcggctgttt ccagaccttt ttgagctggc aggcatccat gacccaccca
     1141 aggcgtttcc ggagctgcgc acccgaaaga ccgcgcgccg cgcccacgaa ccggagatat
     1201 tcctcgacgt tcatgaatcc gtagagcggc acggtctcgg gaaggtagcc gatccgcctg
     1261 cggacgccga ccgggtcctt gaggatatcg aggccgtcga ccgaggcagt gccggaggtc
     1321 gggtaaagat aggtcgtgag gatacgcatg gccgtggatt ttccggcgcc gttcggcccg
     1381 agaagtccga gaatctcgcc cgggttggcc cggaaggaca cgccgtcgag cgccgtcacg
     1441 tgtccgtagc gcatggtcag attcttaacg tcgatcattc aaacctcagt gataagtccg
     1501 ggggttaagc ggtaaagatt gcaagtgccg gacgcggggt cccctgatca cttacaactt
     1561 gccacttatc actcatttac cgcctgcgcc cgaaaatacg cagaaggaac aggaacatgt
     1621 tgacgaaatc cagataaagg gccagcgcgc cgaggatcgc gagctggtcc gcggctcctt
     1681 gcgcattctg atggatccac ttgagtttct gggtgtcgta ggcggtcaac ccgatgaaga
     1741 ccgcgacccc gacatacgaa agaatccagt ggaaggccgg ggcccggaag aacattccga
     1801 caaggctcgc gatcagaacg ccccagatcg ccatcgcgca aaaacttccg acagaggtca
     1861 ggtcctgttt tgtgacccac ccgtagaggc tcacgcccgc gaacatggcg gcgcagataa
     1921 aaaatgtcac cgaaatggac gtcgcggtgt agacaaggaa gatgctggag agtgtgacgc
     1981 cgttcagcgc cgagtagacc agaaacagca tcgtcgcggc cgaagccgac agacgccgga
     2041 tattggccga gagccagaac acgagaaaca gttccacgat gaagaagagc cacatccctc
     2101 ccttcacgag aaacatcagg aacccggcgt tcaggacggt tgcgaaggcc gcgagcccgg
     2161 taagaccaag ccccatcgcc atccagaaat agactcgttg tatgaatgag gaaagcgcgg
     2221 cggcgccgga ataaggctga gaattgtggt ttggatccat ggttcactcc gttggcgacg
     2281 ggccccggtc agcgggggcc caagttgaga gagtcttaat gactttcgtt aaaacttcct
     2341 tccgggaatt gcgtatattc tatacgaaag gggtcatcgc ctcaaggctg accgagcgaa
     2401 tccgtcgttc ccgtcgtttc gtccgccgcc ggggactgtc cttccgttac cgaagccgca
     2461 ggcgcttgcc catctgccga cgacaccgcg gacaccagga attccctgat tttgcggacc
     2521 ggccgggcca ggatcgcccg gtcgccctgc tccacgatcg gccgctgaag cagatcagga
     2581 tagatctcga tcaggtcgag atattgggcg tccgtcaggt tcttctcgcc gagccgcagg
     2641 tcctcgtaaa tctgttcttt tgtgcggagc aattcacgca acggcactcc cattttccgg
     2701 gcaagctcgc agagcttcgc cttcggaatc cgcgtcacgt aataatcgac agattcgatc
     2761 tcggcgccgg cgtctttgag aacctggcag acctctttac aggcggagca ggacggtttc
     2821 tgatagatcg tgattttttc catgataact cccggtgcgg agtacgcggt acggggtacg
     2881 gcaaaaaccc tttcgctcct gctccgtact tcgtacgccg tactagcttt ttagaaaata
     2941 caaccccacg taaactttcg ggtccacgag ccgcccgctc ttttccatcg caagcagcca
     3001 ggactccgcc tccgcgagcg ggacttcatg gacggtgatc gactccgtct cgtccccgcc
     3061 ccctttatgt tttttgacga tcccggtcgc gagataaaaa gtgataattt cggcactgag
     3121 accgcttgag accggacctt cggcgacccg ctcgagcttc ccggcttcat agccggtctc
     3181 ttcaaggagt tcgcgtttcg ccgcggactc catcgattcg gggtcgtgag acgtcttgtc
     3241 gttcacgaga cccgcgggcc attcgatcac ggaggccttg acgggcttgc ggtgctgttc
     3301 gacaaaaacg acccttccgt cctccgtccg cggcacaatc accacgatcc cgtcgcagtt
     3361 cgtccgccgg acatattccc agttgccttc acggaccagg tccagaaact ttcccttatg
     3421 aaggatctct tgcgtcacct tatctctccc tcaaaccgcg gcggcgccgt acaggtcgcc
     3481 gcgaagatcg ctttccgcca ccagcttgcc gttttcttca tcaaaacaaa ccatttgccc
     3541 ttcgccgaga atacctttga tgggattgtc tcgctgcaca cggt
//
LOCUS       DAOOBX010000055         3579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_29074_length_3579_cov_3.423950, whole genome shotgun sequence.
ACCESSION   DAOOBX010000055 DAOOBX010000000
VERSION     DAOOBX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29074_length_3579_cov_3.423950"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLY30_01320"
     CDS             <1..476
                     /locus_tag="PLY30_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64771.1"
                     /translation="LTSFLIPLIPILWIFSPNLVYLFAIEIFSGFVWGGFNLCSLNFI
                     YDAVTPGKRLRCISYFTFINGIATFLGAIAGGYLAEHLPPFHGSRILTLFLISGVLRL
                     ASHFILSKRFHEVRVTQKKVSSAELFFSVVGIRPMLGRGRDWGFLSTLRKLPKED"
     gene            complement(503..826)
                     /locus_tag="PLY30_01325"
     CDS             complement(503..826)
                     /locus_tag="PLY30_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64772.1"
                     /translation="MKKPVMILSILIAVTVISFFIAKPFLQSFRSSFRKGYEESFLGN
                     FRESFMKSCVAGDPSPRKTALCACVVDRAVRELSVKQLSDQAFALDYIKKNIAPVCLE
                     METAR"
     gene            complement(854..3382)
                     /locus_tag="PLY30_01330"
     CDS             complement(854..3382)
                     /locus_tag="PLY30_01330"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPW64773.1"
                     /translation="MPKTRKKKTPAASTAEDVKQLWHLAPKDMTKDGIKESFSSCLQY
                     GLGKDRYTATSRDDFMALGMALRDRLVERWIKTQQRYHRQKTKRVYYLSMEFLIGRLL
                     GSYVLNLNLWDEAQQALEEFGLDFDTVRNEEMDAGLGNGGLGRLAACFLDSMATLGIA
                     GHGYGIRYDYGIFNQKIINGYQVESPDEWLKHGNAWEFARPEYAQTIKFYGNTNIYHD
                     KQGKLRVEWVNTEDVIAMPYDMPVPGFRNDVVNTLRLWSARGSEEFNFQYFNDGDYEN
                     AVYEKMFSENISKVLYPNDATSMGRELRLKQEYFFTAASIADIIRRFKTDNSVFQNFP
                     DKAAIQLNDTHPALAVAEFMRILLDEEQLEWDTAWDLTVRTFAYTNHTVMPEALECWP
                     IPMFEKLLPRHMELIYEINDRFLKMLAAEYPGDTDRLRRMSLIEEGNPKRIRMAHLAI
                     IGSHSVNGVSALHTELIKTQLFRDFYDVFPEKFNNKTNGIAHRRWLVKSNRSLTKLIT
                     DAIGDGWEKDLGGLEKLIPIHRDKLLKKKWQQVKLENKKVFADLIHKTQGIMINPESV
                     FDVQVKRIHEYKRQSLFILYAISEYLKLRENPAAHVTPRTFLFGGKAAPGYYMAKLII
                     KFINNVANIVNHDKAIHDKLKIVFLENYRVSLAEKIFPASDLSEQISTAGTEASGTGC
                     MKFMMNGALTVGTYDGANIEIAEAVGLQDIYMFGLRASEVMELKTRGYHPGAYIEKSP
                     VLREIFHLIRSNFFSPAERGIFEPLVQSLTVSDPFLVCADFESYCAIQKQISEDYQDQ
                     DRWTEKAILNVAKSGYFSSDRTICEYARDIWQVPTSFCPRAHRT"
BASE COUNT          725 a         1009 c          994 g          851 t
ORIGIN      
        1 agctcacgag cttcctgatt ccgttgatcc cgattctctg gatattctcg ccgaaccttg
       61 tctacctttt cgcaatcgag atcttctcgg gattcgtctg gggagggttc aatctctgtt
      121 cactgaactt catctacgac gcggtcaccc cgggaaagag gctccgctgc atcagttatt
      181 tcacctttat taacggcatt gcgacattcc tcggagcgat cgcggggggc tacctggcgg
      241 aacatctccc gcccttccac ggctcgcgga tcctcacgct ttttttgatc tcgggcgtcc
      301 tgcggctggc ctcccacttc atcctttcga agcgttttca tgaagtgcgg gtgacgcaaa
      361 agaaggtctc gagcgcggaa ctcttcttca gcgtggtggg gatccgtccg atgctcggca
      421 gggggcgcga ttggggcttt ctttccacgt tgcggaagct tccgaaagag gactgacgcg
      481 gagcccggac acccggccgt tcttaacggg ccgtctccat ctcaagacag actggcgcga
      541 tattcttttt tatgtaatca agggcgaacg cctggtcact gagctgtttc accgacaatt
      601 ccctgacggc ccggtcgacc acgcaagcgc agagcgccgt ttttcgcggg gagggatcgc
      661 cggcaacaca gcttttcatg aaactttccc gaaaattccc gagaaagctt tcctcgtagc
      721 ctttgcgaaa cgaactccgg aaggactgca ggaacggctt cgcgataaaa aaactgatga
      781 ccgtcaccgc gatcaggatg gacaaaatca tgaccggctt tttcatagga tcctttcctg
      841 gctcacctta aggttaggtg cgatgcgccc gcgggcagaa actcgtcggc acttgccaga
      901 tgtcccgcgc gtattcgcaa atcgtccggt cgcttgaaaa atacccggat ttcgcaacgt
      961 tcaggatcgc cttctcggtc cagcggtcct ggtcctggta atcctccgag atctgctttt
     1021 ggatggcgca gtacgattca aaatcggcgc aaacgaggaa tgggtccgaa accgtgaggc
     1081 tctggaccag cggctcgaag attccgcgct ccgccgggga gaagaaattg ctccggatca
     1141 ggtgaaaaat ctccctgagc accggggatt tttcgatata cgcgccggga tggtaacccc
     1201 gcgtcttcag ttccatcacc tcactcgctc gcaggccgaa catgtagata tcctgaaggc
     1261 cgaccgcctc cgcgatctcg atattagcgc cgtcatacgt cccgaccgtg agcgcgccgt
     1321 tcatcatgaa cttcatgcaa ccggtccccg aggcctcggt gccggccgtt gagatctgct
     1381 cggaaagatc gcttgccggg aatatcttct cggccaggga caccctgtaa ttttcgagga
     1441 atacgatctt gagcttgtcg tggattgcct tgtcgtggtt gacgatattg gcaacattgt
     1501 tgatgaactt gatgatcagt ttcgccatgt aataacccgg cgccgccttg cccccgaaaa
     1561 gaaaagttct cggcgtgaca tgcgcggcag ggttctcgcg cagcttgagg tattccgaga
     1621 tggcgtacag gataaaaagg gactggcgct tgtactcgtg tatccgcttc acctggacgt
     1681 caaacacgga ctccggattg atcatgatcc cttgtgtttt gtggatgagg tccgcaaaaa
     1741 ccttcttgtt ctcgagcttc acctgctgcc atttcttctt cagtagtttg tcgcggtgga
     1801 tcgggatcag tttctcgaga ccgcccaggt ctttttccca tccgtcgccg atcgcatccg
     1861 tgatcagctt cgtgagcgaa cggttcgatt tcacaagcca gcgccggtgc gcgatcccgt
     1921 tcgtcttgtt gttgaacttt tccggaaaaa catcgtaaaa atccctgaag agctgggtct
     1981 ttatcagttc ggtatggagc gcggagacac cgttcacgga gtgcgacccg atgattgcga
     2041 gatgggccat ccggatccgc ttggggttcc cctcttcgat cagggacatg cggcgaagac
     2101 ggtccgtatc gccgggatat tcggcggcca acatcttgag gaaccggtca ttgatctcgt
     2161 agatcagttc catgtggcga gggagcagtt tttcgaacat cgggatgggc cagcactcaa
     2221 gcgcctcggg catcacggtg tggttcgtgt aggcgaacgt ccgcacggta aggtcccacg
     2281 ccgtatccca ctcgagctgt tcctcgtcca gcaggatccg catgaactcg gcaaccgcga
     2341 gcgcgggatg cgtgtcattc agctggatcg cggccttgtc gggaaaattc tggaaaacgg
     2401 aattgtccgt cttgaaccgg cggatgatgt cggcgatgga agcggccgtg aagaaatact
     2461 cctgcttcag ccgaagctcg cgtcccatcg aggtcgcgtc gttgggataa agcacctttg
     2521 agatattctc cgagaacatc ttctcgtaaa cggcgttctc atagtccccg tcgttgaagt
     2581 actggaagtt gaactcttcc gaaccgcggg cggaccagag ccggagggtg ttcacgacat
     2641 cgttcctgaa accggggacc ggcatgtcgt agggcatcgc gatcacgtcc tcggtgttca
     2701 cccactccac gcggagtttc ccctgtttgt catgatatat gttcgtatta ccgtagaact
     2761 tgatcgtctg cgcgtactcg gggcgcgcga actcccaggc attgccgtgt ttgagccact
     2821 catcgggcga ttccacctga tacccgttga tgatcttctg gttgaagatg ccgtagtcgt
     2881 aacggatccc gtagccgtgc cccgcgatac cgagcgtcgc catcgaatcg agaaaacagg
     2941 cggcgaggcg tccgaggcct ccgttcccga gacccgcgtc catctcctcg ttgcggaccg
     3001 tgtcgaaatc caggccgaat tcctcgagcg cctgctgcgc ctcatcccag aggttcaggt
     3061 tcagaacata gctgccgaga agccggccga tgagaaattc catggagaga taatagacgc
     3121 gcttcgtctt ctggcgatga tagcgctgct gggtcttgat ccaccgctcc acgaggcgat
     3181 cccggagcgc cataccgagc gccatgaaat catcccggga cgttgccgtg taacggtcct
     3241 tgccgaggcc atactgaaga caggaactga acgactcctt gatgccgtcc ttcgtcatat
     3301 ccttcggcgc gaggtgccag agctgcttga catcttcggc ggtggacgct gcgggtgttt
     3361 tcttttttct tgttttaggc atgctttcct ctcggtgaaa attaaaggat catttcaaat
     3421 ggcggataga ctagcagatt aaaaaaagat atccaagtct ctcatcaggt attgaagtca
     3481 aaaaggtgcg cctgccacgg ttcaagatcg atgtaaagcc ctcgctgggc gagctcggtg
     3541 cccgctcttt cgtaaacctg gtcgttgaga gcgtcccgg
//
LOCUS       DAOOBX010000056         3579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_29078_length_3579_cov_3.047106, whole genome shotgun sequence.
ACCESSION   DAOOBX010000056 DAOOBX010000000
VERSION     DAOOBX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29078_length_3579_cov_3.047106"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3579)
                     /locus_tag="PLY30_01335"
     CDS             complement(<1..>3579)
                     /locus_tag="PLY30_01335"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64774.1"
                     /translation="SLKIFVWILAFDFFRILIWMDHMGLRVATLVNLSFLGMDKLDEK
                     VARFIGPAAAQRYIPEGVKRFTTWAPLLIPFYLPRGAQWDEVWGTAEKIQNAHRGKGL
                     IPALQSMSWPQLVLLIAAAVVLFSVVSMIVRKLHRRAARRKIASYTVANREYRITMKE
                     NTEVYGEVINKECDVTRRSYDLVDPCGRVLYLVDTGKAPDSAERAWPVAGNFPERYFA
                     PSKAEKVDDTLRITNSNQGVRTSIDITLPDQDSTAEIWSITVENLTDRERQLKVVPYL
                     EWVLNGWMHDRFHTQYQRLYPEMQYVNASNAMLSWNKSTKSMGFLASDIAPEGFLTGR
                     VDFIGRARSIWSPRIVETLDFLEAKDTPACPTFDPIGSFLIHLPLAAGAKKTVKLMIG
                     YAKKKEQALEMIAKHLKPDPKKIPSAFQPKKHALLIGHGEIPEGTPQPYYEFKNDGDR
                     LLVKTPYTPRPFDHGMANPVHSVMVTNRGLHTSCNGNSQQNRVTPDWPDTVTREIPSE
                     AIYLYEPDRGEWYSPTHHPLNDTAAKNECEFGVDGTAVFRMSKGNLSTELTVFVPTED
                     PMGVYLLTVRNHEDQARKIRVAPYFQMALAFQPERSGPLVTKYDKALDALFFENPRNT
                     FRTGSAFVSMSIPADNVTTKRGCFFGSGRGPVDPYFVERAEPDALQMTDNAQVAAFVG
                     TLEVPARGERTVAIILGQTDHRKHAIQLVQKYKNMEMVKTTLDETRKWWLGLMGTTTL
                     QTNQPEFDRYLNWLKYQALAERIWARRGFYQTSGAFGFRDQLQDTVNLTWVDPALARK
                     QIILHASQQFIEGDVFHWFFTLTDGRSAFSCRSHASDNLLWIAWGVGEYIRITGDDTI
                     LDEMTSYVTSEFPFAPLPKNKHGWGALYGRSTRADTVYRHCMRSIDTVLDHRMGRNGL
                     PLMGVGDWNDGLDEIGSGGKGESVWLGFFLYYILREMLPAIERKDGVKRKEHYTAKMK
                     DLAAALEKTWRGDRYLRAIHDDGTEIGVKDSGVWEIDALTAAWSVYTGINFDRGVTIF
                     NTALSVLERGSVILLGWPALREDTKPYLGRSSKYPEGVRENGMYCHGVQWLVRAARLI
                     AEEYEKRGDRAKADEYRATSFRLWSKISPLAHVTPEEIEIYGGQPNKQPADFLTTFEP
                     GRMIWNGYTGAAGWMLRQAFEGVAGATLIHNELILPRDISQPRGPIR"
BASE COUNT          651 a         1112 c         1103 g          713 t
ORIGIN      
        1 cctgatggga cctctcggct gggagatgtc cctgggcagg atcagctcat tgtggatgag
       61 cgtcgcgccg gccacaccct caaaagcctg gcggagcatc catcccgcgg cgccggtata
      121 gccgttccag atcatgcgcc ccggctcgaa cgtcgtgagg aagtcggccg gctgtttgtt
      181 cggctgtccg ccgtagattt cgatctcctc cggcgtcacg tgagcgagcg gcgagatctt
      241 cgaccagaga cggaacgacg tcgcgcggta ttcgtccgct ttcgcgcgat caccgcgttt
      301 ctcgtattcc tcggcgatca ggcgcgcggc acggacaagc cactggacac cgtggcagta
      361 catgccgttc tcgcgaacgc cttcgggata cttgctgctc cgtccgaggt acggcttcgt
      421 gtcctcacgc agggccggcc atccgaggag gatgacgctg ccgcgttcaa gaaccgaaag
      481 ggccgtgttg aagatggtga ccccgcgatc gaagttgatc cctgtataga ccgaccaggc
      541 ggccgtcagg gcatcgatct cccacacccc gctgtccttc acgccgatct cggtgccatc
      601 gtcgtgaatc gcgcgaaggt agcggtcacc acgccaggtc ttctccagcg ccgcggccag
      661 gtccttcatt ttcgcggtgt aatgttcctt gcgtttcacg ccatccttgc gctcgatcgc
      721 cggaagcatc tcgcgcagga tgtaatagag gaagaacccg agccacacgc tctcgccctt
      781 ccccccgctg ccgatctcgt cgagaccgtc gttccagtcg ccgacaccca tgagaggaag
      841 accgttccgt cccatgcggt gatccaggac tgtgtcgatc gaccgcatgc agtggcggta
      901 gaccgtgtcg gcccgggtcg aacgtccata aagggcgccc cacccgtgct tgttcttcgg
      961 gagcggcgcg aacgggaact cggacgtcac atacgacgtc atctcgtcca ggatcgtgtc
     1021 atcgcccgtg atgcggatgt actcgccgac cccccacgcg atccagagca ggttgtcgga
     1081 ggcgtgcgac cggcacgaaa aggccgaacg gccgtccgtc agcgtgaaga accagtggaa
     1141 cacgtcgccc tcgatgaact gctgggaggc atgcaggatg atttgcttgc gcgcgagcgc
     1201 ggggtccacc catgtgaggt tgaccgtatc ctgcagctgg tcgcggaagc cgaacgcgcc
     1261 gctcgtctgg tagaaaccgc ggcgggccca gatgcgttcc gcgagggcct gatatttcag
     1321 ccagttgagg taacgatcga actcgggctg gttcgtctga agcgtggtcg tgcccatgag
     1381 cccaagccac catttccggg tctcatcaag cgtcgtcttg accatctcca tattcttgta
     1441 cttctggacc agctggatcg catgcttcct gtggtcggtc tgcccgagaa tgatggcgac
     1501 cgtgcgctcc ccgcgcgccg ggacttccag cgtcccgaca aaagccgcga cctgcgcatt
     1561 atcggtcatt tgcagcgcat ccggttccgc gcgctcgaca aaataggggt ccacgggacc
     1621 ccgcccgctt ccaaagaagc agccgcgttt tgtcgtgacg ttatccgccg gaatcgacat
     1681 cgaaacgaag gccgacccgg tgcggaaggt gttcctcgga ttttcgaaga aaagggcgtc
     1741 cagcgccttg tcgtacttcg taacgagcgg gcccgaacgt tccggctgga acgcgagcgc
     1801 catctggaaa tacggggcga cgcggatctt gcgcgcctgg tcctcgtggt tgcggaccgt
     1861 caggaggtaa acccccatcg ggtcttccgt cggcacgaac accgtcagtt cggtcgagag
     1921 gttccccttg gacatccgga aaaccgccgt tccgtccacg ccgaattcgc actcgttctt
     1981 cgcggccgtg tcgttcagcg ggtgatgcgt cggcgaatac cactcaccgc ggtccggctc
     2041 atacaggtag atcgcctccg acgggatctc acgcgtcacc gtgtccggcc agtcgggcgt
     2101 cacgcggttc tgctgggaat tcccgttaca ggaggtgtga agaccgcgat tcgtgaccat
     2161 cacggaatgc acggggttcg ccatgccgtg atcgaacggg cgcggcgtgt agggggtctt
     2221 gacgagcagc cggtccccgt cgttcttgaa ttcgtaatag ggctgcgggg ttccctccgg
     2281 aatctcgcca tgaccgatca aaagcgcgtg ctttttcggc tggaacgcgg acgggatttt
     2341 tttcgggtcc ggcttcaggt gcttcgcgat catctcaagc gcctgttctt tctttttcgc
     2401 gtagccgatc atgagtttca cggtcttttt cgcgccggcg gcaagcggca gatggatcag
     2461 gaaactgccg atcggatcga aagtcgggca ggccggcgtg tccttcgcct cgagaaaatc
     2521 aagcgtctcg acgatccgcg gcgaccagat gctgcgcgcg cggccgatga aatcgacgcg
     2581 ccccgtcagg aagccttcgg gggcgatatc ggacgcgagg aatcccatgg acttcgtgct
     2641 cttgttccag gaaagcatcg cgttcgaggc gttcacgtac tgcatctcgg gataaagccg
     2701 ctggtactgc gtgtggaaac ggtcatgcat ccacccgttc agcacccatt cgaggtacgg
     2761 gacgaccttg agctggcgct cgcggtccgt caggttctcg acggtaatgc tccagatctc
     2821 ggccgtgctg tcctggtcgg gcagcgtgat atcgatcgag gtccggaccc cctggttcga
     2881 gttcgtgatc cggagcgtgt cgtcgacctt ttcggccttt gaaggagcga aatagcgttc
     2941 cgggaaattg ccggcgaccg gccacgcccg ttcggcgctg tccggggcct tgccggtatc
     3001 cacgagataa agaacgcggc cgcagggatc aacaaggtca taagaacggc gggtgacgtc
     3061 gcattccttg ttgatgactt ccccgtagac ctcggtgttc tctttcatcg tgatgcggta
     3121 ctcgcgattc gcgaccgtgt agcttgcgat cttccgccgg gccgcgcgcc ggtgaagttt
     3181 ccggacgatc atggaaacaa ccgaaaaaag aaccaccgcg gcggcgatca gaagcacgag
     3241 ctgcggccag gacatcgact gcagcgcggg gatcaatccc ttgccgcgat gcgcgttctg
     3301 gatcttttcg gccgtccccc agacctcgtc ccactgcgct ccgcgcggaa gatagaacgg
     3361 gatcagaagc ggcgcccacg tcgtgaaccg tttgacgcct tcgggaatgt agcgctgggc
     3421 ggccgcggga ccgatgaacc gcgcaacctt ttcatccagc ttgtccatcc cgagaaagct
     3481 caggttcacg agcgtcgcga cgcgaagccc catgtggtcc atccagatca ggatccggaa
     3541 gaaatcaaag gccagaatcc agacgaagat cttgaggct
//
LOCUS       DAOOBX010000057         3531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_29567_length_3531_cov_3.600978, whole genome shotgun sequence.
ACCESSION   DAOOBX010000057 DAOOBX010000000
VERSION     DAOOBX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29567_length_3531_cov_3.600978"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PLY30_01340"
     CDS             complement(<1..1071)
                     /locus_tag="PLY30_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPW64775.1"
                     /translation="MSTAARKKSRKSGSKSRSSKYVYFFGSGKAEGDARMKSLLGGKG
                     ANLAEMTNLGIPVPAGFTITTETCDLFYRNGKKWPVGLEQQVRENIAKLEKSMGLKFG
                     DRNNPLLVSVRSGAAVSMPGMMDTVLNLGLNSDVVQGLIVKTENERFAWDSYRRFIQM
                     FGDVVMEVPHDDFEHVLEKKKQQVGAKSDTDLNANDLKDVVRQYKKVYQDYTKEEFPQ
                     DPFEQLVKAVNAVFNSWNCPRALKYRQINEIRGLLGTAVNVQAMVFGNMGETSGTGVC
                     FTRNPSTGENKFYGEYLINAQGEDVVAGIRTPEPIAVLAQKWPVVYKQLDTLRLRLEK
                     HYRDMQDIEFTIQEGKLYILQTR"
     gene            complement(1297..3396)
                     /gene="glyS"
                     /locus_tag="PLY30_01345"
     CDS             complement(1297..3396)
                     /gene="glyS"
                     /locus_tag="PLY30_01345"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPW64776.1"
                     /translation="MPNILVEIGTEELPVAALDVVYDELAARLRQKLAEERIAFGEVT
                     VEATPRRIAVFIRDIAVTQQDRVLEISGPSYEKCYDARGHGTQVLQGFLKSKQAAEKD
                     IEIRELPKGKFIFLKKHEKGKTVVSIFPEILKSVIASLGFPKFMRWDGSGFRFPRPIR
                     WLVVLADSRKIPMQIADVKSGNMSFGHRFLAPQSFTIPKADWKAYVGLLKRKHVQLDL
                     REREEIIRRALKDRFSQREIDEELVHINAHLTEEPFLLQGTFPGEYLDLPAEVLASCM
                     KKNQKIFACYDAKGRPVNRFVAVMNGKRSGLPGIGTDYENVLESRLKDARYFHDADTK
                     EKLEAKKGLLHQLVYLGKLGSMLDKTERLEKLAPVLAEAAGKKELAVDLVRTACLAKI
                     DLMTHLVYEMPDLQGIVGREYAVKSGEKESVAEAIGTQYLPKNLAEDYRSVSRSMTLL
                     GALFGILDRVDLLTGAFGIGIEPTGSQDPFALRRAGGTVVKLIRAFNLHFSIEKLITA
                     NIHLYGERLNKKDHLRERLLKFLQERTVFEAGVKPGTRPFEVLQAVMRTSFDDIAEVF
                     RKYEALAGMDEGVLVKAAKIIQRTANMLKGYGKTPGEPNPEAFTEEQEKRLFEVVRTK
                     SREVATLIERGEYEKGTRFFAQTFAEPLHDFFDHVLVNAEDAAVRENRMALVQAVNRL
                     YAERLADLSLLSRLDEE"
     gene            complement(3400..>3531)
                     /locus_tag="PLY30_01350"
     CDS             complement(3400..>3531)
                     /locus_tag="PLY30_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPW64777.1"
                     /translation="ERPRYIARVRALARKCAEGYLKSREALGFPMLKKTPSAGKAGK"
BASE COUNT          710 a         1026 c          981 g          814 t
ORIGIN      
        1 acgggtctgg agaatgtaga gcttcccttc ctggatcgta aactcaatgt cctgcatgtc
       61 ccgataatgc ttttcaagac gcaaccggag cgtgtcgagt tgtttataga ccaccggcca
      121 tttttgcgcg agcaccgcga tcggttccgg cgtgcggata ccggccacga cgtcttcacc
      181 ctgcgcgttt atgagatact cgccgtagaa cttgttctcg ccggtcgagg ggttgcgggt
      241 gaagcaaacg cccgttccgg aggtttcgcc catattgccg aacaccatgg cctggacgtt
      301 cacggccgtt ccgagaaggc cgcgaatctc gttgatctgg cggtacttga gagcgcgcgg
      361 gcagttccag gaattgaata ccgcattgac cgccttcacg agctgttcga aggggtcctg
      421 cggaaactct tcctttgtat aatcctgata gaccttcttg tactgacgca caacatcttt
      481 caggtcgttc gcgttcaggt ccgtatcgga cttcgcgccg acctgctgtt tcttcttttc
      541 gaggacatgc tcgaaatcat cgtgcgggac ttccatgaca acgtcgccga acatctggat
      601 gaaacggcga taggaatccc acgcgaaacg ttcgttctcg gtcttgacga taagtccctg
      661 gaccacatca gagttgagac cgagattgag caccgtgtcc atcatgcccg gcatcgaaac
      721 cgctgcaccg gaacgcacgg aaacgagcag cgggttattg cggtcaccaa acttcagccc
      781 catcgatttt tcgagtttcg cgatgttctc gcggacctgc tgctcaaggc ccaccggcca
      841 tttcttgccg ttcctgtaga agaggtcgca ggtctcggtc gtgatcgtga aaccggcggg
      901 aacgggaatg ccgaggttcg tcatctcggc gaggttcgcg cctttgccgc cgagaaggct
      961 cttcatcctg gcatcaccct cggctttgcc ggacccgaag aagtaaacat atttggaaga
     1021 cctggatttt gaaccggact tacgggattt cttgcgtgct gcggtactca tgtgtttcgt
     1081 cactcctttt tatagtctga cgcccgcgcc ctccagagaa cggggcgaca gacctgttgg
     1141 tcatttaatg cggcaaaact tttccctacg gtcaggaccg tgaggggttt cggatagccc
     1201 gaaccggccc tatgaatcca ccggagggac gggacgacac tgcatgcccg cgaacatcgg
     1261 tgatgcggca tcgggcaaag aagattcggg attatatcac tcttcgtcaa gtctggatag
     1321 caaagaaagg tcggcaagcc tctccgcgta gagacggtta acggcctgca ccagcgccat
     1381 gcggttctcc cggacggcgg catcttcggc attcaccaga acatggtcga aaaaatcatg
     1441 gagcggctcg gcaaaggtct gcgcaaagaa ccgcgtcccc ttttcgtatt ctccgcgctc
     1501 aatcaaggtg gcgacttccc gggatttcgt gcgaacgacc tcaaaaaggc gtttctcctg
     1561 ctcttccgtg aacgcctccg ggttcggttc acccggcgtt ttgccgtaac ctttcagcat
     1621 attggcggtc cgctggatga ttttggcggc cttgaccaac accccctcgt ccatcccggc
     1681 cagggcttca tattttctga aaacctccgc gatgtcgtca aaggatgtcc gcatcaccgc
     1741 ctgaagcact tcgaacggcc gcgtgcccgg tttgacgccc gcctcaaaga ctgtccgttc
     1801 ctggaggaat ttgaggagcc gctcccggag atggtccttc ttgttcaggc gttccccgta
     1861 aagatggatg ttcgcggtga tcagtttttc gatcgaaaaa tggaggttga aagcgcggat
     1921 cagcttcacg acggttcctc ccgcgcggcg gagcgcgaag gggtcctggc tgccggtggg
     1981 ttcgatgccg atcccgaacg ctcccgtcaa aaggtcaaca cggtcgagga ttccgaagag
     2041 agctcccaga agcgtcatgg atcgcgaaac ggaccggtag tcctcggcaa ggttcttcgg
     2101 caggtattgc gtgccgatcg cctcggccac ggattccttt tcccccgatt tgaccgcgta
     2161 ttccctgccg acgatcccct gaaggtccgg catctcgtaa acgaggtggg tcatcagatc
     2221 gatcttggca agacaggcgg tcctgaccaa atcgacggcc agttctttct ttccggccgc
     2281 ttccgcgagg accggcgcca gtttctcgag cctttccgtt ttatccagca tggatccaag
     2341 cttcccgaga tagaccagct ggtgcaaaag accttttttc gcctccagtt tttctttcgt
     2401 atccgcatca tggaaataac gggcatcctt cagacgcgac tcgagtacgt tctcgtaatc
     2461 ggtgccgatt ccgggaaggc ccgaccgctt cccgttcata acggcaacga acctgttcac
     2521 aggacgaccc ttcgcgtcat agcaggcaaa gatcttctgg ttcttcttca tgcagctcgc
     2581 gagtacttcg gccggcagat caagatactc cccgggaaaa gtcccctgca gcaggaacgg
     2641 ctcttccgtc agatgcgcgt tgatatggac cagctcctcg tcgatctccc tttgactgaa
     2701 acggtctttc agggcgcggc ggatgatttc ctcgcgttcg cggaggtcca gttgcacgtg
     2761 tttgcgcttg agaagcccca cgtaggcctt ccagtcggcc ttgggaatcg taaaagactg
     2821 cggggcgagg aaccggtggc caaagctcat attaccggac ttcacatccg cgatctgcat
     2881 cgggattttc cgggaatcgg ccaggacaac gagccagcgg atcggccgcg gaaaacgaaa
     2941 accggacccg tcccaacgca tgaatttcgg gaatccaagt gaagcgataa cgctcttcag
     3001 gatttcggga aagatcgaaa caaccgtttt tcccttctcg tgttttttga gaaagatgaa
     3061 cttgcctttc ggaagttccc ggatctcgat atccttttca gcggcctgtt tgctcttgag
     3121 aaacccctga aggacctgcg tcccgtggcc ccgggcgtca taacattttt cgtaagaggg
     3181 accggagatc tcaagcactc gatcctgttg ggtgaccgcg atgtcccgga taaagaccgc
     3241 gatgcggcgc ggcgtcgctt ccacggtcac ttcgccgaaa gcgatgcgct cctctgcgag
     3301 cttctgacgg agtctggccg caagctcatc gtaaacgaca tcgagcgccg cgacggggag
     3361 ctcttcggtt ccgatctcaa caaggatgtt cggcatggat cacttccctg ccttcccggc
     3421 ggagggggtt tttttgagca tcggaaatcc cagggcctcg cggctcttga gatatccttc
     3481 ggcgcatttg cgggccaggg cacggacacg ggcgatatat cggggtcgtt c
//
LOCUS       DAOOBX010000058         3518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_29695_length_3518_cov_3.634999, whole genome shotgun sequence.
ACCESSION   DAOOBX010000058 DAOOBX010000000
VERSION     DAOOBX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29695_length_3518_cov_3.634999"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /gene="ppdK"
                     /locus_tag="PLY30_01355"
     CDS             <1..1617
                     /gene="ppdK"
                     /locus_tag="PLY30_01355"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPW64778.1"
                     /translation="RSGKRTAAAAVRTAVEMVKEKMITQKEALLRLEPKQLDQLLHPT
                     FDPKQQKTKEPIATGLPASPGAATGKVVFSAEEAEACAEKGETVILVRIETSPEDIGG
                     MNVAKGILTSRGGMTSHAAVVARGMGKCCVAGVSGINIDYKAKQFTAGKVTVREGDFI
                     SLDGSLGYVYEGKLNTMEPTLSGDFGTLMKWADAARRLKVRTNSDTPLDATVARRFGA
                     EGIGLCRTEHMFFEGDRIIAVREMILADDTAGREKALAKLLPMQRADFEGIFEAMKGL
                     PVTIRLLDPPLHEFLPQEDANQREMAQIMNVPVEVIKRKVSALHEFNPMLGHRGCRLG
                     ITYPEIYAMQVRAIIEAACNLKKKGVKVVPEIMIPLIGTRKELDILKQLSIDTINKVF
                     GEKKTKVEYMIGTMIEVPRAALTADRVAETAEFFSFGTNDLTQMTLGFSRDDSGNFMG
                     AYLEQGIFEKDVFQSIDEEGVGSLVKLAVEKGRKTKKDLKIGICGEHGGDPDSVGFCH
                     RVGMNYVSCSPYRVPIARLAAAQAALKEKK"
     gene            1691..2851
                     /locus_tag="PLY30_01360"
     CDS             1691..2851
                     /locus_tag="PLY30_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPW64779.1"
                     /translation="MAGKKKKKKSGSIRRVQKKQTRKKASRKRRKRSRLVSRRFKKPG
                     APAISGKIEALLQQEIAFQKEAHDIIRKETSAQGVSERRQRSRSHEEDEESDSEDAMD
                     QTLDRTPMKIYLDQIEHIPLLTPEEEISLARKIQTGGPAGEDSRQKMIRSNLRLVIAI
                     AKRYTHMGLPFSDLVEEGNIGLMRAVEKFDPGKGYRFSTYASWWIKQGMMRSLSNHGK
                     TIRIPVYMYDIISKWRRVRDALTQRLNRTPTRREIAKILEIPISKVREIENIVNKPSS
                     LNTPLSLDGAAELIDLIEDDVARKPDAQVGEVLKSERVQKLLDRLDDRERQILILRFG
                     FGEHDPHTLEEVAKNFGVTRERIRQLEGVALKKIRAQLILEGDHFDNYLSHD"
     gene            2889..3410
                     /locus_tag="PLY30_01365"
     CDS             2889..3410
                     /locus_tag="PLY30_01365"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPW64780.1"
                     /translation="MSIASLKARVRDIPDFPKKGILFRDITPLLKEPASFREIVARLT
                     AAVRKSGAKVVVGIESRGFIFGAPVAAKLGIGFVPIRKKGKLPWRTRQVACTLEYGQE
                     ILEIHADAVKRGQKVVVIDDLLATGGTAQAAAKLVEKLGGKVALLAFVIELEFLKGRE
                     KLGRYDALSLLKY"
BASE COUNT          817 a          954 c         1062 g          685 t
ORIGIN      
        1 cgtagcggaa aacgtacggc ggccgctgcg gtccgcacgg cggttgagat ggtgaaggag
       61 aagatgatta cgcaaaaaga ggcgctcctg cgtctcgagc cgaagcagct ggaccagctt
      121 ctgcatccca ccttcgatcc gaagcagcag aaaacaaagg aacccatcgc gacggggctc
      181 ccggcctccc ccggcgccgc gaccggtaag gtcgttttca gcgctgaaga agccgaagca
      241 tgtgcggaga agggcgaaac ggtcatcctc gtccgtatcg agacctctcc ggaagatatc
      301 ggcggcatga acgtcgcgaa gggaatcctg acctcccgcg gcggtatgac ctcgcacgcg
      361 gccgtggtcg cgcgcggcat ggggaaatgc tgcgtcgccg gtgttagcgg gatcaacatt
      421 gattacaagg cgaaacagtt tacggccggg aaagtgaccg taagggaggg agattttatt
      481 tctctcgacg gcagccttgg ttatgtctat gaaggcaagc tcaacacgat ggaaccgacg
      541 ctttcgggcg attttggcac gctgatgaag tgggccgatg ccgctcgccg gcttaaggtc
      601 cgcacgaaca gtgatacgcc ccttgatgcg acggtcgccc gcaggtttgg cgccgaaggt
      661 atcgggcttt gccgcacgga acacatgttc ttcgagggtg accggatcat cgcggtccgt
      721 gagatgatcc tggccgatga caccgccggt cgcgaaaagg ccctcgcaaa gctcctgccg
      781 atgcagcgcg cggactttga agggatattc gaggcgatga aaggactccc tgtgacgatc
      841 cgccttctgg atccgccgct tcacgagttc ctcccgcagg aagacgcgaa ccagagggaa
      901 atggcgcaga tcatgaacgt gccggtcgaa gtgatcaagc ggaaagtgag tgcgcttcac
      961 gagttcaacc cgatgcttgg tcatcgaggc tgccgtcttg ggatcacata tcccgagatc
     1021 tatgccatgc aggtccgcgc gatcattgaa gcggcttgca atctcaagaa gaagggcgtc
     1081 aaggttgttc cggagatcat gatccccctg atcgggacca ggaaagagct cgatattctg
     1141 aaacagcttt cgatcgacac gatcaacaag gtgtttgggg aaaagaagac caaggtcgag
     1201 tacatgatcg ggacgatgat cgaagtgccg cgcgcggcgc tcacggcgga ccgtgtcgcc
     1261 gagaccgcgg agttcttctc gttcgggacg aacgatctta cgcagatgac cctcgggttc
     1321 tcgcgtgatg attccggcaa cttcatgggc gcctaccttg agcagggaat cttcgaaaag
     1381 gacgtgttcc agtccattga cgaagagggc gttggttccc tggttaaact tgcggtcgag
     1441 aaaggccgta aaacgaagaa agatctgaaa atcggcatct gcggcgaaca tggcggtgat
     1501 ccggattcgg tgggtttctg tcaccgggtc gggatgaatt acgtcagttg ttccccgtac
     1561 cgtgttccga tcgcccggct tgcggcagcg caggcggcgc tcaaggagaa gaaataactg
     1621 agggagatcc ctcgataagc tcgggattaa ataaccccgg ccttacagcc ggggttattt
     1681 gactgctctc atggcgggaa aaaagaaaaa aaagaaatca ggttcgatcc gtcgggtcca
     1741 gaagaaacag acccggaaga aggcaagccg gaagcgcaga aagcgttccc ggcttgtttc
     1801 ccgtcgtttt aaaaagccgg gggcccccgc gatctccggc aagatcgaag ccttgctgca
     1861 gcaagaaata gccttccaga aggaagccca cgatatcatc cgcaaggaaa cctctgccca
     1921 aggggtctcg gaacgtcggc agcggtcccg ttcccatgag gaggacgaag agtccgactc
     1981 ggaagacgcg atggaccaga cgcttgaccg cactccgatg aagatttatc tcgaccagat
     2041 cgagcatatt ccgcttctga ccccggaaga agaaatctcg ctggcccgga agatccagac
     2101 gggaggtcct gcgggggagg attcccggca gaagatgatc cgttcgaacc tgcgtcttgt
     2161 gatcgcgatc gccaaacgct acacgcatat gggactcccg ttctccgatc ttgtggagga
     2221 aggcaatatc ggcctcatgc gcgcggtgga gaagttcgat ccgggaaaag gctaccgttt
     2281 ctcgacgtat gcgtcctggt ggatcaaaca ggggatgatg cgctcgctct cgaaccatgg
     2341 caagacgatc cgcattccgg tctacatgta tgacattatt tccaagtgga ggcgggtccg
     2401 tgatgccctg acgcagcggc tcaaccgcac tccgactcgc agggagatcg ccaagattct
     2461 cgagataccg atctcgaagg tccgcgagat cgaaaatatc gtcaacaagc cgagttcgct
     2521 caatacgccc ctcagtctgg acggcgcggc tgaattgatc gatctgattg aagatgacgt
     2581 cgcgagaaag cctgatgccc aggtcggcga ggtcctgaaa agcgaacggg tccagaaact
     2641 tctcgaccgt ctcgatgacc gggagcggca gatcctgatt ctccgcttcg ggttcgggga
     2701 acatgacccg cataccctgg aagaggtggc caagaatttc ggagtgaccc gcgaacgcat
     2761 ccgccagctc gaaggtgtgg cgctaaagaa gattcgtgct caactcatcc ttgaaggtga
     2821 tcatttcgac aattatcttt cacacgactg atccgttccc cgatcagtca atccccagga
     2881 ggaattgcat gagtatcgct tcactcaaag ccagggtccg cgacatcccg gattttccga
     2941 agaagggcat cctctttcgg gatatcaccc cgttgctcaa ggagccggcc tctttcaggg
     3001 agattgtggc gcgcctgacc gcggcggtcc ggaagtccgg ggcgaaggtt gtggtcggga
     3061 tcgagtcgcg cgggttcatt tttggcgcgc cggttgccgc caaactcggc atcggatttg
     3121 ttccaatcag gaaaaagggg aagcttccct ggcgaaccag acaggtcgcc tgcacactcg
     3181 agtatggaca ggaaatcctc gagatccacg cggatgcagt caagcgcggt cagaaagtcg
     3241 tcgtgatcga tgacctcctc gcgactggcg gaacggcaca ggccgcggcg aaactcgtcg
     3301 agaagctcgg cggcaaggtc gcgctgctcg cgttcgtgat tgaacttgaa ttcctcaaag
     3361 ggcgcgaaaa actcggccgt tacgatgcgc tttctctgtt gaagtattaa cgccaaacga
     3421 taatatatcc ccgtttcagc ggcgtttaat aaccggcggt cacgccccca tagctcaatt
     3481 ggatagagca tctccgtcct aaggagaggg ttgcatgt
//
LOCUS       DAOOBX010000059         3515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_29728_length_3515_cov_3.349422, whole genome shotgun sequence.
ACCESSION   DAOOBX010000059 DAOOBX010000000
VERSION     DAOOBX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29728_length_3515_cov_3.349422"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..781
                     /locus_tag="PLY30_01370"
     CDS             86..781
                     /locus_tag="PLY30_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW64781.1"
                     /translation="MNNAAAILSASGLRKDYDTVTGSLEILRGVNLEVCQGEMLCITG
                     KSGCGKSTLLHLLGGLDRPTDGKLFFRGREMTGMPEREIAKIRCRNIGFVFQFYHLLP
                     ELTVHENVLLPTLIAKKKDSGWVKEVLKRVKLWNRRTHFPSELSGGEKQRVAIARALA
                     NRPDVVLCDEPTGNLDEETAGTITSLLAELNQKEGHTFLIVTHDEEIARTGSRSLTLH
                     EGQLMPASNVFKH"
     gene            814..1719
                     /gene="ilvE"
                     /locus_tag="PLY30_01375"
     CDS             814..1719
                     /gene="ilvE"
                     /locus_tag="PLY30_01375"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HPW64782.1"
                     /translation="MELQIYIDGKWYPRSEAKVSVFDHGLLYGDGVFEGIRAYDGIVF
                     KLEEHLKRLWESAHTIMLEIPMTPREMEKAVIETLRRNKLTSGYIRLVVTRGTGDLGL
                     SPFLCKKPTVFIITDKIALYPADLYQKGLEIITVPTVRNLPEAVNPSIKSLNYLNNIM
                     AKIEARNGGCMEALMLNHQGYVVECTGDNVFMVKKTGKGSAVKDCTLLTPPSYLGALK
                     GITRDAIIEIARKLKLPFAERILTRHDFFNADEVFLTGTAAEVIPVVKIDGRVVGSGK
                     PGKITKLLLEDFHALVRKDGVRFSL"
     gene            1742..2263
                     /locus_tag="PLY30_01380"
     CDS             1742..2263
                     /locus_tag="PLY30_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPW64783.1"
                     /translation="MICNVCGTKEATIHLTEVVSGQMAEVHICEQCAEEKGTDFKTYF
                     NFGDFFTGFAGIGKLLETPGEKTAVACKSCGMTFEEFSRQGRLGCAQCYTAFQRQLEA
                     VIKQVQRAGVHVGKKPSRIPKDVRRAHDLRQLQDRLRKCIQNEAFEEAARLRDEIRQI
                     EDKNKRPRKTKND"
     gene            2256..3320
                     /locus_tag="PLY30_01385"
     CDS             2256..3320
                     /locus_tag="PLY30_01385"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HPW64784.1"
                     /translation="MTENGSSPAQSVNWLRATGPENDVVVSSRIRLARNLANYPFLIK
                     MTPDQRIALIAEMEKAVRASSFLKKANMILTKEIGEIDRQFLLERYLISNALVREDGA
                     SAVVITPDEMVSLMMIEEDHLRLQVFQSGLNLAEAWRIADEIDMELEKSLSYAFDATL
                     GYLTACPTNVGTGIRASCMLHLPSLVITKQIQKVLQALSKLNLAVRGLYGEGTQALGS
                     FFQFSNQITLGQPETEIISNLEGVIKQVITHEREAREYLRKKRKTKMEDQVWRALGVL
                     KSARLISSQEAIQLLSLVQTGVDTGIIEGRVTHRDLNQLFLWIQPGHLQKMHGSELSA
                     ADRDIQRADRIRTFFSKLSL"
BASE COUNT          812 a          922 c         1053 g          728 t
ORIGIN      
        1 gggctggcgt tcattcttgc cgtgatcgcg gcgctttatc ccgcgcaccg ggcagcaagc
       61 ctcaaaccgg tggaggcgct tcgttatgaa taacgcggcg gcgatcctca gcgcatccgg
      121 actgcggaag gattatgaca ccgtgaccgg tagcctcgaa atcctgcgag gggttaatct
      181 ggaggtttgc cagggggaaa tgctttgcat cacgggtaaa tcggggtgcg ggaagtcgac
      241 cctgcttcat ctcctgggcg gtcttgaccg accgacggac gggaagcttt tctttcgggg
      301 gcgtgagatg accggcatgc ccgaacgcga gatcgcaaaa atccgctgtc gcaatatcgg
      361 tttcgtgttc cagttctatc atctgttgcc ggagctcacg gtccatgaaa atgtattact
      421 ccccacactt atcgcgaaaa aaaaggactc gggctgggtc aaggaggttc tgaaacgtgt
      481 gaagctctgg aaccggagga cgcatttccc gtccgagctc tccggcgggg agaaacagcg
      541 cgtggcgatc gcccgggccc tcgccaatcg accggacgtc gttctttgtg acgaaccgac
      601 cggcaatctc gacgaagaaa cggccgggac gattacgtcg ttgctcgcgg aattgaacca
      661 gaaagagggt cacacatttc ttatcgtgac ccatgatgaa gaaattgccc ggacagggag
      721 ccggtcgctg acgcttcacg aaggtcagct gatgccggct tcgaatgttt ttaaacattg
      781 atcgcagcgg atccttaaac aaggagattc aagatggaac ttcaaatcta tatcgacggt
      841 aaatggtatc ccaggagtga agcgaaagtg tctgtcttcg atcacggcct tctttacgga
      901 gacggtgttt ttgaggggat tcgcgcctac gatggaatcg tcttcaagct cgaggagcat
      961 ctcaaacggc tctgggagtc ggcccatacg atcatgctcg aaattccgat gacgcccagg
     1021 gaaatggaaa aagccgttat tgaaaccctt cgcaggaaca agctgacgag cggctatatc
     1081 cgactggttg tcacgcgcgg cacgggggac ctcggactat cgccctttct ctgcaagaaa
     1141 ccgacagttt tcattatcac ggacaagatc gcactttatc cggcggattt gtaccagaaa
     1201 gggctggaga ttatcaccgt tccgaccgtc cggaatctcc ccgaggccgt gaatccgtcg
     1261 atcaaaagcc tgaactatct caataatatc atggccaaga tcgaggcaag gaacggcggt
     1321 tgtatggaag cgctgatgct caaccaccag ggctacgtcg tagaatgcac gggtgacaat
     1381 gtgttcatgg tcaagaaaac cggcaagggg tcggccgtga aggattgcac gcttttgacg
     1441 cctccgtcct atctcggagc gctcaagggg atcacgcgtg atgcgatcat cgagattgcc
     1501 cgcaaactca aactgccttt cgcggaaaga atcctgacgc ggcatgattt ttttaacgcc
     1561 gatgaggtat tcctcacggg aacggcggcg gaggtcatcc cggtcgtcaa gatcgacgga
     1621 cgcgttgtcg gaagcggtaa gccgggcaag attaccaaac tgcttttgga ggattttcac
     1681 gcgctggtcc gcaaggacgg ggtccggttc tccctttgat cgggccggga gcgaagcgaa
     1741 catgatctgc aatgtgtgcg ggacaaagga agcgacgatt cacctgaccg aggttgtgag
     1801 cggtcagatg gcggaagtgc atatctgcga gcagtgcgcc gaagagaagg ggacggattt
     1861 caagacctat ttcaactttg gcgacttctt cacgggtttc gcggggatcg gaaaacttct
     1921 tgagacgccg ggggaaaaaa ccgcggtcgc ctgcaaatcc tgcggtatga ccttcgagga
     1981 attctccaga cagggacgcc ttggatgcgc gcagtgttat accgcgtttc agcgtcagct
     2041 tgaagccgtg atcaaacagg tccagcgggc gggggtccat gtcggcaaaa aaccgtccag
     2101 aatcccgaag gatgttcggc gcgctcacga tctccgccag cttcaggacc gtctgcgcaa
     2161 atgcatccag aacgaggcgt ttgaggaagc ggcgcgcctt cgcgacgaga tcaggcagat
     2221 cgaagacaag aacaagcgcc cgaggaaaac aaaaaatgac tgagaacggt tcttcgccgg
     2281 cgcagtcggt caactggctt cgggccacgg gtcccgagaa cgatgtggtc gtcagctccc
     2341 gcatacggct cgcgcgcaat ctcgcgaatt acccgttcct gatcaagatg acgcccgatc
     2401 agcggatcgc cctgatcgcg gagatggaaa aggcggtccg cgcctcgagt tttctgaaga
     2461 aggccaacat gatcctgacg aaggagatcg gcgagatcga ccggcagttc cttctcgagc
     2521 ggtatctgat ttcgaacgcg ctggtgcgcg aggacggtgc gagcgcggtt gtgatcacgc
     2581 cggatgaaat ggtcagcctc atgatgatcg aggaagacca cctccggctc caggtcttcc
     2641 agtcggggct gaaccttgcc gaggcgtggc gcattgccga cgagatcgat atggaactcg
     2701 aaaagagcct tagttacgct tttgacgcga cgcttggtta cctgacggcc tgcccgacga
     2761 atgtcgggac cggtattcgc gcctcctgca tgctgcatct gccgagcctt gtgattacga
     2821 aacagatcca gaaggtgttg caggcgcttt caaaactgaa cctcgcggtg cgaggccttt
     2881 acggcgaagg gacacaggcg ctcgggagtt ttttccagtt ctcgaaccag atcacgctcg
     2941 gacagcctga gacggagatc atctcgaatc tcgaaggagt cattaaacag gtaatcacgc
     3001 atgagcggga ggcgcgggaa tatctccgga agaaacgcaa aacaaaaatg gaagaccagg
     3061 tgtggcgggc gctcggcgtt ctgaaatcgg cgaggctcat ctcgtcccag gaagcgatcc
     3121 agcttttgtc gcttgtgcag accggtgtcg ataccgggat catcgaaggc cgggtgaccc
     3181 accgggacct gaaccagctt tttttatgga tccagcccgg gcatctccaa aaaatgcacg
     3241 ggtcggaact cagcgctgcg gatcgcgaca ttcaacgcgc tgaccggatc cggacgttct
     3301 tctcaaagtt gtcactttaa cccatgagga taccgtttat gaagaagccc cttctcgttc
     3361 tcatcctttc aatattgtgg gtgccggcga gcgtgttcgc ggaaaatgtg acagctgatt
     3421 cgaggatcag tcaggtgacg gtgtattcga atgatgcctt tgtcacgcgc gcggcaacgg
     3481 tcgaggtcaa tccgggcgac accagggtcg tgttg
//
LOCUS       DAOOBX010000060         3481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_30066_length_3481_cov_3.845301, whole genome shotgun sequence.
ACCESSION   DAOOBX010000060 DAOOBX010000000
VERSION     DAOOBX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30066_length_3481_cov_3.845301"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PLY30_01390"
     CDS             complement(<1..1094)
                     /locus_tag="PLY30_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW64785.1"
                     /translation="MSAHHSDIIGKIVIYFAKSKITPLIIAVSLVVGVFAVINLPREE
                     EPQISVPMFDIFVKFPGADSKEVEQRIVTLGERKLWEIPGVEYVYSTIEPEGALIIVR
                     FKVGEDPEKSLINLYTKIYSNLDFLPQGSAQPFIKVRSIDDVPILTLTFHSKARDPVT
                     LRRTVANIRNIVNAIPDVSETAITGGRKRQFQVFFDEEKLKARLLNPLEIAGIIRSAN
                     VKQGAAHLESSPEIEHVESDSFFRTKADLENLVVGVSAGAVVMLKDIAAVADGPDEEE
                     RATEIFFGPASRETEKGPFGAVTLAVSKRKGANAARLSEEILHAIRKLPEATLPKDIG
                     MTVTRDYGETAKEKSDELLFHMAIAVFGVSV"
     gene            complement(1133..2530)
                     /locus_tag="PLY30_01395"
     CDS             complement(1133..2530)
                     /locus_tag="PLY30_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW64786.1"
                     /translation="MLTGKKASAFFAAIFFMIQVASADTASVPVPASDPGMTFLDCYE
                     KVLRYYPALRKRYERLDQAKAGRNLVISELFPKIRGVYEMQTSDDPVFVFGSLLRQES
                     FTSNNFALPALNTPRHRTNHRFGIEGDMLLFDSFNTISKIREARRLVKSADLEADFTE
                     MEAAIVTLESYLGILLANTLYNVAVEVKRASDKDLQQAKDLNDKGMILGADYYAAKVI
                     AAGIEREVNRLSALRKTSYMLMNILMGESPEMTWNAVGKFPETAQDKSALEAWLAQAY
                     QKRQDLAALDQMLDAQGIERTRQKTSFLPKVYGFASLDENSHDWHTGGRGFSIGMKGT
                     MDFFDPGLPGRVKDANAKYKALKADRDALRDAIARDLVGELARYETATLDAPVMKGAC
                     ADAKEASELTAKLYQEGRKSIADLLEMRRAYFETSAGRENLRLALELQYAKLLFLSGQ
                     LTEDGIRQVNARLKG"
     gene            2672..>3481
                     /locus_tag="PLY30_01400"
     CDS             2672..>3481
                     /locus_tag="PLY30_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HPW64787.1"
                     /translation="MTKNLFGTIAKAGLLLSLIAILPSTGSAGERPLFARADKSTPVL
                     NTPDFRSVFGGGDGRTLKRDASGLIREVEFIALPGTAFVVETSLKVRGATVYRVRTPE
                     YPSPASGLFVDSRFVSVSAKPFPPRMKILPRARQILDRMLSHEGARYVWGGNAPGGIP
                     ELLTFYPPAGALSPATKNMWSLRGLDCSGLLYEATGGATPRNTSDLVTFGKPVAVAGL
                     TPGQIAAKLRPLDLIVWKGHVIIVLDEEHTIESCMGCSPRHTPQRPFFEVEF"
BASE COUNT          659 a         1066 c         1007 g          749 t
ORIGIN      
        1 acgctcacgc cgaagaccgc gatcgccata tggaacagca gttcgtcgga tttctccttc
       61 gccgtctcgc cgtagtcgcg cgtcacggtc atcccgatat ccttcggaag cgtcgcctcc
      121 ggcaatttcc ggatcgcgtg gagaatctcc tccgacagtc gggccgcgtt cgcgcccttg
      181 cgcttggaga ccgcgagcgt caccgcgccg aagggacctt tttccgtttc ccgggaagcc
      241 gggccgaaaa aaatctccgt cgcgcgttcc tcttcgtcgg gaccgtccgc gaccgccgcg
      301 atgtctttca gcatcacgac cgctcccgcg ctcacgccga cgaccaggtt ctcgagatcg
      361 gccttcgtgc gaaagaagga gtccgattcg acatgttcga tctcgggaga actttcgaga
      421 tgagccgcgc cctgtttcac gttggcggag cggatgatcc ccgcgatttc gagcgggttc
      481 agcaggcgcg ccttcagttt ctcctcgtca aagaacacct ggaactgccg tttgcggccg
      541 cccgtgatcg cggtctccga aacgtcgggg atcgcgttca cgatgttccg gatattcgcg
      601 accgtccggc gaagcgtgac ggggtctctt gccttcgaat gaaacgtgag cgtgaggatc
      661 ggcacgtcgt cgatcgaccg gaccttgatg aacggctgcg ccgaaccctg gggcaggaaa
      721 tcgagattgg agtagatctt cgtgtagaga ttaatcaggc tcttttcggg gtcttcgccg
      781 accttgaacc ggacaatgat gagcgcgcct tcgggttcga tcgtggagta gacgtactcg
      841 acgccgggga tctcccagag cttgcgttcg ccgagcgtca cgatccgctg ctcgacctcc
      901 ttcgaatccg cgccgggaaa cttgacgaag atatcgaaca tcggaaccga aatctgcggc
      961 tcctcctcgc gcggcaggtt tatgaccgcg aagacaccga cgacgagcga taccgcgatg
     1021 atgagcggcg tgatcttcga tttcgcgaag tagatgacga tcttgccgat gatgtccgaa
     1081 tgatgtgcgc tcattatccc tcaaccgaat ccaacctcgc aggttggatt ggttatcctt
     1141 tcagtcttgc gttcacctgg cggatgccgt cttccgtaag ctggccggaa agaaaaagaa
     1201 gtttcgcgta ctggagctca agcgcgagac gcaggttttc gcgcccggcc gaggtctcga
     1261 aataggcccg gcgcatctcg aggaggtccg cgatcgattt gcggccttcc tggtaaagtt
     1321 tcgccgtgag ctccgatgcc tccttcgcgt cggcgcaagc ccctttcatg acgggcgcgt
     1381 cgagcgtggc ggtctcgtag cgcgcaagct ccccgacgag atccctcgcg atcgcgtcgc
     1441 ggagcgcgtc gcgatcggcc ttgagcgcct tgtatttcgc gttcgcgtcc ttcacgcgtc
     1501 cgggaaggcc cggatcgaaa aagtccatcg ttcccttcat tccgatcgaa aagccccggc
     1561 cgccggtgtg ccagtcgtgg ctgttttcat ccaggctcgc gaacccgtag accttcggga
     1621 ggaaagaggt cttctgacgc gtccgttcga tcccctgcgc gtcgagcatc tggtccaggg
     1681 ccgcgaggtc ctgacgcttt tgataggcct gcgcgagcca cgcttcgagc gcactcttgt
     1741 cctgcgcggt ctcggggaat tttccgacgg cgttccacgt catctccggg ctttccccca
     1801 tcaggatgtt catcaacatg taagaagtct tccgcagggc gctgagccgg ttcacttccc
     1861 gttcgatccc cgccgcgatc accttcgccg cgtaataatc cgcgccgagg atcatcccct
     1921 tgtcgttgag gtctttcgcc tgctgcaggt ccttgtcgga ggcccgtttg acctcgaccg
     1981 cgacgttata aagcgtgttg gcgagcagga taccgagata gctctcgagc gtcacgatcg
     2041 cggcttccat ttccgtaaaa tcagcttcga ggtcggccga cttcacgagc cggcgcgctt
     2101 cgcggatctt cgagatcgta ttgaacgaat cgaaaagcag catgtccccc tcgatgccaa
     2161 accggtgatt ggtccggtga cggggggtgt tgagcgccgg gagcgcaaag ttgttcgagg
     2221 tgaaggactc ctgacgcagg agactgccga acacgaacac cggatcgtcc gaggtctgca
     2281 tttcgtaaac gccgcggatc ttcgggaaaa gttcggagat gacaaggtta cggcccgcct
     2341 tcgcctggtc cagccgttcg tagcgcttgc ggagcgcggg ataatacctg agaaccttct
     2401 cataacaatc gagaaaggtc atcccggggt ctgaagcggg cacaggcacg gaggccgtgt
     2461 ccgcggacgc gacctgtatc ataaaaaaaa tagcggcgaa gaacgccgac gccttttttc
     2521 cggtcaacat gcctcacatc ttaatcggtt cttccgcctt tgaaaacgtt tttcatccgt
     2581 cctttcggaa gggattctcc tcactttttt caagaaccgg gaagtgatac gatacccggg
     2641 tcggaatatc cgatcaggca tgaacaggat catgacaaaa aatctttttg gaacaatcgc
     2701 aaaggcggga ctgcttcttt ccctgatcgc aatcctccct tcaacaggga gcgccgggga
     2761 acgtcctctt ttcgcccgtg ccgataaatc gactcccgtg ctgaacacac cggatttcag
     2821 gtccgtgttc ggaggcgggg acgggaggac tctgaaacgg gatgcctcgg ggctgatccg
     2881 cgaggtcgag ttcatcgcct tgcccggaac ggcctttgtg gtcgaaacct ccctcaaggt
     2941 ccgcggcgcc acggtctacc gcgtccgcac gccggaatat ccttctcccg cgagcggcct
     3001 tttcgtcgac agccgtttcg tgtcggtttc cgcgaaaccc ttccctcctc gcatgaagat
     3061 cctgccgcgc gcgagacaaa ttcttgaccg gatgctgagt cacgaaggcg cgaggtatgt
     3121 gtggggcggc aatgcccccg gaggcatccc cgaactttta acgttttatc cgcccgcggg
     3181 ggcgctttcc ccggcaacaa aaaacatgtg gtctctccgc ggcctggatt gctcgggtct
     3241 tctctacgaa gccaccggag gcgcaacgcc tcgcaacaca tcggatctcg tcactttcgg
     3301 aaaacccgtc gcggtcgcgg gcctgacccc gggacagatc gcggccaaac tccggccgct
     3361 cgatttgatc gtctggaaag gtcatgtgat catcgtactc gatgaagaac acacgatcga
     3421 aagctgcatg ggctgctccc cgagacacac tccccaacgt ccgtttttcg aggtggaatt
     3481 c
//
LOCUS       DAOOBX010000061         3480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_30073_length_3480_cov_4.236788, whole genome shotgun sequence.
ACCESSION   DAOOBX010000061 DAOOBX010000000
VERSION     DAOOBX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30073_length_3480_cov_4.236788"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>3480
                     /locus_tag="PLY30_01405"
     CDS             21..>3480
                     /locus_tag="PLY30_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64788.1"
                     /translation="MTIKGTQILQDGKSVTGYFSLLLDSGGNALWISDPLAGSAGLAT
                     VNAYNGLGSNGAFRAILDNAGAWKVYSMRELSSGRIGGFSGESRAVRDKIGEMNAELS
                     EGKKLTGYEKIRNAWVDDQGNAISADLAAKYNMKETGYTQFYDRTGKSGFLLNGIDLG
                     VLLTDKVIGLRGWGEQPGALALILVNDERVRGEFGCGEVLAVTDLNRDDPPAALKNMK
                     SIEHSENWSVKVNGTTAGGRLEDFALNVLGVRESTTFTDLDGRTYTIANGEFLDGALA
                     KGTGAFSNFAMAFAETKSGLRGLVLEIGQHAEMVAGTHGTMTSGNASEFGPVRPETRT
                     GAFYASGIDVVGSPEGFTPEQIEAAKTLVRGSGMAISMEWRSDDGKITSGVLLNTRAT
                     QGIFVLDGAFSGQGVKDAFTIVKHAGLDGADATFETYNVDGIRAELRSFGNAAGLKLG
                     DGQEYASTAGYVATYGKSVYGTSDVETGIFKAVFEAYGLALPPDFGKIGTGTILLKDG
                     TTIAASISKEGGIAGYSLNGADFLSLSTLADGRSMLAADAYLKKGSDGKYSVVRNGGA
                     LGTEEAAGKTSVTATAAGLTDSASFDKQISFTLDGKGYLLGLSDGKVTAILTGENLSL
                     AMLTADLSGIASNVVFLQTDAFKALMSVDGQQALVVKDASGNVVGGYNKANGESLRKK
                     TGAELEIERSIIKGEGTVRLENGSLREVDASVAHANLALVAAADKDGNVLRIFFEYGQ
                     DFAVFNGVVGGPDDLPGLQYYMSMDGALLLRRSGESLFAYQVNGDGSKDLLGGFDVRK
                     GSDTARTYSWEAVSAANAPTGYGSDYELKKAAKVLVSKTSDAKGNDTTLRVFLGASNQ
                     VLHTSIGGLLVSGFATRGGDEYYLQLRNAAAGLYQVTNLGKGTSTQERLDRGAGNVGF
                     SRNERGVLTVTFTDKNGGIHKLAQVGASLSSGEGTTRSLDVYENLSGYLTADEMNVIQ
                     SEFGSPVFLGLMTGGTHVLLGFSAAGKFTGFDGVVGEFGSLTGALVLSEKGKEVWKFT
                     DSKTEVVQTRVSEDMTNADKSGVRDDSPLKKVTESVSSIVEVTLADGSKATDKARQAA
                     MNALAGFGVFEGTRFSDGAGNAWFTANTGGAALLSAKTVLNAPLVFAGVGEKNQGHLL
                     LVSRSSE"
BASE COUNT          698 a          905 c         1182 g          695 t
ORIGIN      
        1 agatgccggt tacccgggaa gtgaccatta aaggaacaca gattcttcag gacgggaagt
       61 ccgttaccgg ttatttctcg ctcctcctcg attccggtgg gaacgcactc tggatatcag
      121 atccgcttgc gggttcggcc gggcttgcaa cggtcaacgc ctataacggt ctcggctcga
      181 acggagcctt tcgcgcgatc cttgataatg cgggggcgtg gaaggtgtat tccatgcgcg
      241 agctttcttc cggcaggatc ggcggttttt ccggggagtc ccgggcggtc cgggacaaga
      301 tcggcgagat gaacgccgag ttgtccgaag ggaagaagct caccgggtat gagaagatca
      361 ggaacgcttg ggtggatgat cagggcaacg cgatcagcgc tgaccttgcc gcgaaataca
      421 atatgaagga gacggggtac acgcagtttt atgaccggac gggcaagagc gggtttcttt
      481 tgaacggaat cgaccttggc gtgttgttaa cggacaaggt gatcggactc cgtggctggg
      541 gcgaacagcc gggggccctc gctttgatcc tcgttaacga cgagagggtc cgcggagagt
      601 tcggttgcgg cgaggtgctc gcggtgacgg acctgaatcg ggatgacccg cccgcggcgc
      661 tcaagaatat gaaatccatc gaacattccg agaactggtc ggtcaaggtg aacggaacga
      721 ccgccggtgg ccggctcgag gatttcgcgc tcaacgttct tggggtcagg gaatccacca
      781 ctttcacgga ccttgacgga aggacgtata cgatagcgaa cggagagttc ctggacggcg
      841 ctttggcgaa ggggacggga gcgttcagta actttgccat ggcgttcgcg gagacgaagt
      901 cgggccttcg gggattggtt cttgagatcg gccaacacgc cgaaatggtc gcgggaacgc
      961 acgggacgat gacgagcggg aacgcttcgg agttcgggcc cgttcgcccc gaaacaagga
     1021 cgggtgcgtt ttacgcgagc gggatcgatg ttgtgggatc tccggaaggt tttactcccg
     1081 aacagatcga ggccgcgaag accctggtgc gcggctcggg tatggcgatc tccatggaat
     1141 ggcggagtga cgacgggaag atcacgagcg gggttttgtt gaacactcgc gcgacgcaag
     1201 ggatcttcgt cctcgacggc gcgttcagcg gtcagggcgt caaggatgcc tttacgatcg
     1261 tcaagcacgc ggggctggat ggcgcggacg cgacgttcga gacatacaac gtcgatggta
     1321 ttcgcgctga gctgcggagc ttcgggaatg ccgcgggact gaagctcggc gacggccagg
     1381 aatacgcgtc gaccgcgggt tatgtcgcga cttacggaaa gtcggtctac ggcacttcgg
     1441 acgttgagac cggcatcttc aaggcagtat tcgaggccta tggattggcg cttcctccgg
     1501 atttcggaaa gatcgggacc ggaacgatcc tgctcaagga tggcacgacg atcgcggcgt
     1561 cgatctcgaa ggaaggcggt atcgcgggct attcgctgaa cggggcggac ttcctttcgc
     1621 tttcgaccct ggccgacggc cggagcatgc tggcggcgga tgcgtatctc aagaaaggtt
     1681 cggacggaaa gtactccgtc gtccggaatg gcggggcact tggcacggaa gaagcggcgg
     1741 gcaagacgag cgtgacggcg acggccgcgg gattgacgga ttccgcttct ttcgacaaac
     1801 agatttcatt tacgcttgac ggcaagggtt atctcctcgg tctcagcgat ggaaaggtca
     1861 ccgcgatcct gacgggggag aacctgagcc tcgcgatgct gaccgccgat ctctccggga
     1921 tcgcctcgaa cgtcgtgttc ctgcagacgg atgccttcaa agcgctcatg tcggtcgacg
     1981 gccagcaggc cctggtcgtc aaagacgcct ccgggaatgt tgtgggcggt tataacaagg
     2041 cgaacgggga gagccttcgc aagaagacag gcgctgaact cgagatcgaa aggtcgatca
     2101 taaaagggga aggcacggtc cggctcgaga acggttccct cagagaggtt gatgcgagcg
     2161 tcgcgcacgc gaatctcgcg ctggtcgccg cggcggacaa ggacgggaac gtcctcagga
     2221 tctttttcga atacggacag gatttcgcgg tttttaacgg cgtggtcggc ggtccggacg
     2281 accttccggg gcttcagtat tacatgtcca tggatggggc gctgttgttg cgccgctcgg
     2341 gcgagagcct cttcgcgtat caggtcaacg gggacggaag taaagacctg cttggcggat
     2401 tcgatgtccg caaaggttcg gacacggcgc gtacttattc ctgggaggct gtctcggccg
     2461 ccaacgcccc gacgggttac ggcagcgatt atgaactgaa gaaagccgcg aaggtcctcg
     2521 tcagcaagac ctcggacgcg aaagggaatg acaccacgtt gcgtgtcttt cttggcgcat
     2581 cgaaccaggt cctccacacc tccatcgggg gtctccttgt ttccggtttc gccaccaggg
     2641 gcggcgacga atactacctg cagctcagga atgccgcggc gggactctat caggtgacga
     2701 accttggcaa gggcacttcg acgcaggagc gccttgatcg cggcgccggt aatgtcggat
     2761 tctcccgcaa tgagcggggt gttctgacgg tcacgttcac ggacaaaaac ggcgggatac
     2821 acaagcttgc gcaggtcggc gcgtctttgt cgtccggtga agggaccacc cgcagtctgg
     2881 atgtttatga aaacctttcg gggtatctca ccgccgacga gatgaatgtg attcaatccg
     2941 agtttggttc tccggtcttc ctcgggttga tgacgggcgg gacgcatgtt cttctcggtt
     3001 tttcggcggc cggtaaattt acgggatttg acggtgtggt cggggagttc gggtcgttga
     3061 cgggcgccct ggttttatct gaaaagggta aagaggtctg gaaattcact gactcgaaga
     3121 ccgaggtcgt ccagacgcgt gtatcggagg atatgacgaa cgccgacaag tcgggtgtgc
     3181 gcgatgattc gccgctcaag aaagtgacgg agagcgtttc gtcgatcgtg gaagtcacgc
     3241 tcgcggacgg cagtaaggct accgacaagg cccgtcaggc ggccatgaac gcgctggccg
     3301 gattcggggt tttcgaaggc acccggttct cggacggtgc gggcaacgcg tggttcacgg
     3361 cgaacacggg cggcgcggcg ctgctctccg ccaagacggt tctgaacgcc cccctcgttt
     3421 ttgccggggt gggcgaaaag aaccagggac atttgttgct tgtttcccgg tcgagcgaac
//
LOCUS       DAOOBX010000062         3407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_30929_length_3407_cov_3.190632, whole genome shotgun sequence.
ACCESSION   DAOOBX010000062 DAOOBX010000000
VERSION     DAOOBX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30929_length_3407_cov_3.190632"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLY30_01410"
     CDS             <1..368
                     /locus_tag="PLY30_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HPW64789.1"
                     /translation="VHVTRSPMAGTVTCQEHVPGKHINVLLPGSAEKNESNWIGIRGD
                     GRSALVRQITGAIARRIYSDVGPGVRVERGGKLGVICYGSTVELYIPKRLFEPSVKVG
                     QGVRTGETVLGSWKEGVRA"
     gene            365..1117
                     /gene="pssA"
                     /locus_tag="PLY30_01415"
     CDS             365..1117
                     /gene="pssA"
                     /locus_tag="PLY30_01415"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPW64790.1"
                     /translation="MKNRFYFSTAAFPNMITMLNLIAGFFSILLATSGKYEFAVWLIF
                     LALILDSLDGHVARIFGNATDFGRELDSLADLVSFVVAPCFLAATVFSPVMSVWMLLV
                     IVCFLSAGAFRLARYNINPTRGGYFEGLPTPAAAMTVSMTILAFHKNAWQSDPSFVIS
                     AVFLMTAISFLMVSEIPYPKVSAVKFKVWCPLFFLQLFLAIFFFFFFNAETAISVVFL
                     AFLAIAPFYCTARNGACADVSEAGQDGDLPCS"
     gene            1108..1713
                     /locus_tag="PLY30_01420"
     CDS             1108..1713
                     /locus_tag="PLY30_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPW64791.1"
                     /translation="MLLGFIAGFLGSSLGWQINLLAIQRGVRRGKLAALLVGCGAVTA
                     DLIFFWFAFTGAKTLISHTEHWGWFRAAGIVLLLVLAARAYFVHGRPRPRTQKVIAGN
                     PTRNFLVGFLVVIFNPAVFLMWLGILTFLIGHFPEARRPAFKWCFCGGFILGSLSWFG
                     PLAFVLLAKIREWDEAHLHTLSRLLAAVLVAVAFLLVLEKI"
     gene            1916..2950
                     /locus_tag="PLY30_01425"
     CDS             1916..2950
                     /locus_tag="PLY30_01425"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HPW64792.1"
                     /translation="MAVPPRKKKTEGKIKSSRSARTTHRAETNDRTPAVRTPRKKAPV
                     TRAPKTIKLPEALEKPAIVPLSAAKVRLPPVPQTELPENYGDNQIYLLVRDPHWIYSY
                     WEIQKDHQDEVLKKIGGSWDGVTSILRVFDVTDGSMNPPYFDIVLQHAVRSWYINVQP
                     NHAYVVEIGLLHRDGRYASLARSNTVRTPRSGMSEVIDEQWMSVDWDKMYALSGGFQV
                     GKSSAELRKLMEERLKGAISSGSGAGLVSSMASPVKIKKRGFFFWLECELIIYGGTEP
                     DATVTMQGRTVKLRPDGTFTLRYALPDGKFTFDCHAHSSDGVEEREITPVIGRSTKRP
                     APIIKRDFKK"
     gene            2954..>3407
                     /locus_tag="PLY30_01430"
     CDS             2954..>3407
                     /locus_tag="PLY30_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPW64793.1"
                     /translation="MEKGFLSIVLHAHLPFVRHPEHEFFLEELWLYEAITETYVPLIH
                     MMEGLERDRVPWRLTMSLTPSLMSMLRDGLLQDRYVRHIDKLIELVSKEIERTRFMPQ
                     FHETALHYYWEFTRAKDTFVNRYHRDLTQAFRRFQDKGHLEIITCGATH"
BASE COUNT          613 a          990 c          991 g          813 t
ORIGIN      
        1 acgtccacgt gacgcgctca ccgatggcgg gcacagtgac ctgtcaggag cacgtccccg
       61 gcaaacacat caacgtgctg ttgcccggtt cggcggaaaa gaacgagtcg aactggatcg
      121 gaatccgcgg ggacggccgc agcgccctgg tccgtcagat caccggcgcg atcgctcgcc
      181 gcatctattc cgatgtcggg cccggtgtcc gggtcgagcg gggcggaaaa ctgggcgtca
      241 tttgttacgg ctcgaccgtc gagctttata tcccgaagcg attgtttgaa ccgtccgtga
      301 aagtcgggca gggcgtcagg acgggggaga ccgttctcgg gtcgtggaag gaaggagtcc
      361 gggcatgaag aaccggttct atttttcgac ggctgctttt ccgaacatga ttacgatgct
      421 gaatctgatc gccggttttt tttcgatcct gctcgcgacc tccggcaagt atgagtttgc
      481 ggtctggctg atctttctcg cgctgatcct cgattcgctg gacggtcatg tggcccgcat
      541 tttcgggaac gcgaccgatt tcggccgtga acttgattca ctcgccgatc ttgtgtcctt
      601 tgttgtcgct ccctgttttc tcgccgcgac ggtcttttcg ccggtcatgt cggtctggat
      661 gcttctggtc atcgtctgtt tcctgtccgc gggggcgttc cgtctcgcgc gctacaacat
      721 caatccgacc cgcggcgggt attttgaagg gctcccgacg ccggcggcgg cgatgacggt
      781 ctcgatgacg atcctggcgt tccacaagaa cgcctggcag agcgatccct cgttcgtgat
      841 cagcgccgtg ttcctgatga ctgcgatcag ctttctcatg gtgagcgaga tcccgtatcc
      901 gaaggtgtcc gccgtgaagt tcaaggtgtg gtgcccgctt ttttttctcc agctttttct
      961 cgcgatcttc tttttctttt tctttaatgc cgagaccgct atttcggtgg tgttcctcgc
     1021 gtttctcgcg atcgcgcctt tctattgcac cgccaggaac ggtgcgtgcg ccgatgtctc
     1081 cgaagccggg caggacggag atctcccatg ctcctaggtt tcatcgcagg gtttctcggg
     1141 tcaagtctcg gctggcagat caacctgctc gcgatccagc ggggggttcg tcgcgggaaa
     1201 ctggcggcgc tcctcgtcgg ctgcggtgcc gtgacggcgg atctcatctt cttctggttt
     1261 gcgttcaccg gggcgaagac gctgattagt cacaccgaac attggggatg gttccgcgcg
     1321 gccggcattg ttcttttgct ggtcctggcg gcccgggcgt attttgtgca cgggcggccc
     1381 cgtccccgga cgcagaaagt gattgccggg aatccgacgc ggaatttcct ggtagggttc
     1441 ctcgtcgtga tcttcaaccc cgcggttttc ctgatgtggc tcggtatcct gaccttcctc
     1501 atcgggcatt ttcccgaagc ccggcgtccg gcctttaaat ggtgcttttg cggggggttc
     1561 atcctcgggt cgctttcctg gtttggacct ctggcgttcg tcctccttgc caagatcagg
     1621 gaatgggacg aagcgcatct tcataccctg tcacggcttt tggcggcggt gctggtggcc
     1681 gtcgcgttcc tcctcgtgct ggaaaagata taaaatcctt cttttattac tttctgttgc
     1741 cacctttttg acgctttgat atacttgggc cgcctccctc gtctgaatgc ggtcgaaggt
     1801 atcggtgaat ctcggagtta gccgttcgtt ttgtcgttgt cttgatctgt ttctttcaaa
     1861 aatgaatcgt taccgctgtc gaggcggaat gatgttttta tgaggggttg tgaccatggc
     1921 tgttcctccg aggaaaaaga agaccgaagg caagatcaaa agttccaggt ccgcacggac
     1981 gacgcatcgg gcagaaacga atgaccgcac gccggccgtc agaaccccgc gcaagaaagc
     2041 ccctgttaca cgcgctccca aaacgatcaa gcttcccgaa gccctcgaaa agcccgcgat
     2101 cgttcccctc agcgcggcca aagtccggct gccgcccgtg cctcaaacgg agctcccgga
     2161 aaattacggc gacaaccaga tttatcttct cgtccgcgat cctcattgga tctactctta
     2221 ttgggagatc caaaaggacc atcaggatga ggttctgaag aagatcggcg gaagctggga
     2281 cggtgtcacc tcgatcctcc gcgtttttga tgtgacggac ggttcaatga acccgcctta
     2341 tttcgacatc gtccttcagc acgcggttcg cagctggtac atcaatgtgc agccgaatca
     2401 cgcctatgtc gtcgagatcg ggctcttgca ccgcgatggc cgctatgcct cgctcgcgcg
     2461 ttcgaatacg gtccggacgc cgcgttccgg gatgtccgaa gtgatcgatg agcagtggat
     2521 gtccgtcgac tgggacaaga tgtacgcgct ttccggcggg ttccaggtcg ggaagagctc
     2581 ggctgaactc cgcaagttga tggaagagcg actcaagggc gcgatcagct ccggatcggg
     2641 cgcgggcctg gtttcgagta tggccagccc ggtcaagatc aagaaacgcg gatttttctt
     2701 ctggctcgaa tgcgaactga tcatttatgg ggggaccgaa cccgacgcga cggtcacgat
     2761 gcaggggagg accgtcaagc tccgcccgga cggcaccttc acgctgcgct atgcgctgcc
     2821 cgacggaaag ttcacgtttg attgtcacgc gcattcctcc gacggcgttg aggaacgcga
     2881 gatcactccc gttatcggac gcagcacgaa aagacccgcc ccgatcatta aacgggattt
     2941 caagaagtag tccatggaaa aaggttttct ttccatcgtt cttcacgcgc accttccgtt
     3001 cgtgcgccac cccgaacatg agttcttcct ggaagaactc tggctctatg aggccattac
     3061 cgagacctat gtcccgctca ttcacatgat ggagggcctg gaacgcgacc gcgttccgtg
     3121 gcggctcacg atgtcgctca cgccctcgct catgtcgatg cttcgcgacg gactcctgca
     3181 ggaccgctac gttcgccaca tcgacaagct gatcgaactg gtcagcaagg agatcgagcg
     3241 cacgcgattc atgccgcagt tccacgaaac ggccctccac tactattggg agtttacgcg
     3301 cgcgaaagac acgttcgtga atcgctatca ccgggacctc acgcaggcgt tccgccggtt
     3361 ccaggacaag ggacatctcg agattattac ctgcggcgcg acgcacg
//
LOCUS       DAOOBX010000063         3394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_31078_length_3394_cov_3.261456, whole genome shotgun sequence.
ACCESSION   DAOOBX010000063 DAOOBX010000000
VERSION     DAOOBX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31078_length_3394_cov_3.261456"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLY30_01435"
     CDS             complement(<1..490)
                     /locus_tag="PLY30_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPW64794.1"
                     /translation="MKKLIIYDLDGTLVDTRQDIINSVRHALRELNGPHLTDDEIKEC
                     VGTGLHSLIRRVFRTEDEKLAERGAKIYRQHYVEHMLDHSRLYPGAKDMLEFFRDRTQ
                     AVITNKPNPFSTQILEALGVAHYFIDILAGDNGLPFKPDPASLFCLMGEVKARPEETL
                     FVG"
     gene            complement(715..2124)
                     /locus_tag="PLY30_01440"
     CDS             complement(715..2124)
                     /locus_tag="PLY30_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW64795.1"
                     /translation="MHNLRRFFESFRVRVTLALILAIFLMGVASDIAVQRIALRAQFE
                     QLRENLMTTASAIASSVDADEIASIPMDRSGVETPAFRALFDRFLRLKQENPLIGFVY
                     VLKPAENPLEWQFVVDADPYLAYRKGVTAYPGDRYDVTRFPEISEALRGPVAEKKVQP
                     DDWGTMLSGYAPVRDKSGKAVAVVGVDTLASDISRMQKKIHASVLLAFVAGLAIFLPL
                     GIWFSTRLTASLRTLSDGIRRVSGGDLSYRITVTGHDEISELSRSFNQMAADLAHAYQ
                     KNQDYFYGIIRTLVMIVEARDPYTRGHSERVSEFAVEIAKRLGFSPERLEMLKQTAIL
                     HDIGKLGIHEAILSKKEKLTEAEWELLRNHPMIGEDILKPVLLGPEMLAAVRGHHERH
                     DGNGYPDKLAGEDIHVFARILSVADAYDAMTSKRLYRQVLSQESAVAELRKHSGTQFD
                     PQIVDIFIAILKERKSAAS"
     gene            complement(2128..>3394)
                     /locus_tag="PLY30_01445"
     CDS             complement(2128..>3394)
                     /locus_tag="PLY30_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPW64796.1"
                     /translation="EAQVAAEEGGETVRMMTIHAAKGLEFPVVIVAGMGSEGKNPDPK
                     SIIAHANEGFAIRLRNDLTRAPEKSRFYQILDEKMILREKEERKRLFYVACTRAKSRL
                     ILSGIREIRKKPKDRYADMLTWMDWMDAIAGKTGIPAACDTDRAVPFFGQKPENLREK
                     IRKALETMRPAQEAGPVPEPAREAPRLRSIDLPVSAYVLFSKDPQAFWRTYQIGWQET
                     REDVRDAEEAEEEERPGYRASDFGTAMHSLLERIDLTDPDRYLEQDVLDREFGSFGKD
                     GIRDGREILKAFFGSALFKRLRKADRVEREVDFTLNGRRGLIHGKIDALFRESDGAWH
                     VLDYKTAAGDRASAEASGHDLQIGIYALAVSRILKEPVRSGMIYYLKNAAEVTTDFPQ
                     APDVLDGIEKRICDLQQKILDFCNRKMVH"
BASE COUNT          678 a         1035 c          929 g          752 t
ORIGIN      
        1 cgccgacaaa cagcgtttcc tccgggcggg ccttgacttc ccccattaaa cagaaaagcg
       61 aggcgggatc cggtttgaac ggaagaccat tatctcccgc caggatatcg atgaaataat
      121 gcgcaacgcc gagcgcttcg agaatctgcg tggaaaaggg gtttggtttg ttcgttatca
      181 cggcctgtgt ccggtccctg aagaactcca gcatgtcctt cgcgccggga taaagtcggg
      241 agtgatcgag catgtgttcg acgtaatgct gccggtaaat cttcgctccc cgttccgcga
      301 gtttttcgtc ctccgtccgg aacacccggc ggatcagcga atggagcccc gttcccacgc
      361 attccttgat ctcgtcgtcc gtgagatggg ggccgttcag ctcccggagg gcatggcgga
      421 cgctgttgat gatatcctgg cgcgtatcca ccagcgttcc atcgaggtca tagataatga
      481 gttttttcat gcaatctctc ccgtgatcct tgccgcgaat tcggcggcag gagttgcaac
      541 caggatattt gtttttaagg aacgccgcgt ggacacattc ggcctacacg ccccgggatt
      601 gaaccccctt cggtcatcca accgatcggg gccgctgtgt tgccatccgt ttcgggactt
      661 ccgttcaaaa cgatgatctt ttccgtgaca tgaagagaat cccgcagaat acggtcagga
      721 agcggccgat ttcctttcct tcaggatcgc gataaaaata tccacgatct gcgggtcgaa
      781 ctgggtgccg ctgtgtttgc gaagctccgc gaccgccgat tcctggctca gaacctggcg
      841 gtaaagccgc tttgaagtca tggcatcata ggcgtcggcc acggaaagga tcctggcaaa
      901 gacatggata tcctcccccg cgagtttgtc gggataaccg ttcccgtcat ggcgttcatg
      961 gtgaccgcgg acggccgcaa gcatctcggg cccgagaagg accggcttca ggatatcctc
     1021 gccgatcatc ggatggttcc tgagaagttc ccattccgct tcggtcagtt tctctttttt
     1081 ggagaggatc gcctcatgga tgccgagttt gccgatatca tgaaggatcg ccgtctgttt
     1141 cagcatctcc agccgttcgg gagagaagcc gagccgtttc gcgatctcga ccgcgaattc
     1201 cgaaacccgt tccgagtgac ctcttgtgta ggggtccctg gcttccacga tcatcaccag
     1261 cgtccggatg atcccgtaga aataatcctg gtttttctga taagcgtgcg ccaggtcggc
     1321 cgccatctgg ttgaacgagc gggacaattc cgagatctcg tcatgccccg tcaccgtgat
     1381 cctgtagctc agatccccgc cgctcacacg ccggataccg tcggaaagcg tccggagtga
     1441 cgccgtcagc cgggtcgaga accagatccc gaggggaagg aagatcgcca gaccggccac
     1501 aaaagccagc aggacgctcg catgaatctt tttctgcatc cggctgatgt ccgaagcaag
     1561 tgtgtcgacc cccaccacgg cgaccgcttt gccgcttttg tccctgaccg gcgcgtaacc
     1621 ggagagcatg gtcccccaat catcgggctg gacttttttt tccgcgaccg gtccgcgaag
     1681 cgcctcgctg atctcgggaa accgggtcac atcgtaccgg tcacccggat acgcggtcac
     1741 tcctttccgg tatgccagat acggatccgc gtcgacgacg aattgccatt cgagagggtt
     1801 ctccgccggt ttcaggacat agacaaaacc gatcaaggga ttttcctgct tcagcctcaa
     1861 aaaccggtca aaaagggcac gaaacgcggg cgtctcgaca ccgctccggt ccatcgggat
     1921 cgatgcgatc tcgtcggcat cgaccgagga ggctatcgcg ctcgcggtgg tcatgagatt
     1981 ctcgcgaagt tgttcgaatt gggcgcgaag agcaatgcgc tggaccgcga tatcgctcgc
     2041 cactcccatc agaaagatgg cgaggatcaa cgccagcgtg acgcggacgc gaaagctttc
     2101 aaaaaaacgt ctgaggttat gcatgtctta gtgaaccatt ttccggttac aaaagtccag
     2161 gattttttgc tgcaaatcac aaatccgttt ttcgataccg tcgagaacat caggggcctg
     2221 aggaaagtcc gtggtcactt ccgcggcatt cttgaggtaa tagatcatgc ctgagcggac
     2281 cggctctttc aggatccggg agaccgcgag cgcgtaaatc ccgatctgca ggtcatggcc
     2341 gctcgcttcc gcagacgcgc gatctcccgc cgccgtttta taatcaagca catgccaggc
     2401 gccgtcgctt tcacgaaaca gcgcgtcgat ctttccgtgg atgagccccc gtcgaccgtt
     2461 cagcgtgaaa tcaacctcac gttcaacccg gtcggccttc cggagacgct taaaaagagc
     2521 cgaaccgaag aacgctttca agatttcgcg gccgtcccgg atgccgtctt tcccgaacga
     2581 cccgaattcc cggtccagca cgtcctgctc cagataccga tcgggatccg tgagatcgat
     2641 ccgttccaaa agcgaatgca tggccgttcc gaaatcagag gcccggtaac ccggcctttc
     2701 ctcttcttcg gcttcttccg cgtcccggac atcctcccgt gtttcctgcc acccgatctg
     2761 gtaggtccgc cagaaggcct gcgggtcttt cgaaaaaaga acgtaggccg aaacgggcag
     2821 gtcgatgctc cggagtcgcg gggcctcgcg cgccggctcc ggcaccggtc cggcttcttg
     2881 cgccggcctc atggtttcca gggccttccg gatcttttcg cgcagattct ccggtttctg
     2941 tccaaaaaac gggaccgcgc gatcggtgtc acaagcggca gggatgccgg tcttcccggc
     3001 gatcgcgtcc atccagtcca tccaggtcag catatcggcg taccgatctt ttggcttttt
     3061 ccggatctca cgaatcccgc tcaggatcag ccgggacttc gcacgggtac acgccacgta
     3121 gaaaagccgc ttccgctcct ccttttcgcg aagaatcatt ttttcgtcca gaatctgata
     3181 gaaacgggat ttttcgggcg ctcgcgtcag gtcgttccga agacggatcg cgaaaccttc
     3241 gtttgcgtgc gcaataatgc tctttggatc cggattcttt ccttcggagc ccatccccgc
     3301 gacgatcacg acggggaatt ccagaccttt cgccgcgtga atggtcatca tccgcaccgt
     3361 ttccccgccc tcttcggccg ccacctgcgc ctcg
//
LOCUS       DAOOBX010000064         3378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_31283_length_3378_cov_3.071020, whole genome shotgun sequence.
ACCESSION   DAOOBX010000064 DAOOBX010000000
VERSION     DAOOBX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31283_length_3378_cov_3.071020"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLY30_01450"
     CDS             complement(<1..376)
                     /locus_tag="PLY30_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPW64797.1"
                     /translation="MSDPIDISALKIPGFLHPQKKVLLHVCCAPCCASILHRMLAVGL
                     EPTVFFYNPNIYPREEYNLRKNEVIHYAGKRGIPFIDRDGDGGLWFETVKGHELEPER
                     GERCGLCFDMRLGKSAAYASENG"
     gene            complement(389..694)
                     /locus_tag="PLY30_01455"
     CDS             complement(389..694)
                     /locus_tag="PLY30_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64798.1"
                     /translation="MITDELLTILCCPETQQPLTPVDPILITKVNGAIAKGTLRTRGG
                     QKVTRLTDGGLVRDDRRYLYAVRQGIPVMLREESISLSNLESSGNRTRDTETPISYT"
     gene            complement(757..2286)
                     /locus_tag="PLY30_01460"
     CDS             complement(757..2286)
                     /locus_tag="PLY30_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPW64799.1"
                     /translation="MIGSIASDRPYEFPPTDLLRMPKAEKIEGDDLAANSKAIEETLK
                     EFDIDVKVVEVEQGPKITLYEVLPAPGVRANSISAYEDDLAMALKATSIRLIIPIPGK
                     QTIGIEVPNAVSRPIVIREMIEAPDFKSSSQKLPLLLGKDTAGKPMIADLASMPHILI
                     AGTTGSGKTVCINSVIAGFLYHCTPSQLKLVMVDPKMVELAVYNKIPHMLSPVVTDVR
                     KAANTLNWVVQEMERRYRLFAAVGVRNIQTFNEREISEEDKKQLAEAPKDSENVVPAS
                     LPYIVVIIDELADLMLVAQDKVETAITRLAQLSRAVGIHLILATQRPSVDVITGVIKA
                     NFPARVAFKVASVVDSRTILDNKGADKLLGRGDMLFIQPGSDNMIRGQAPLIVDDEIN
                     RIVGFVSKQGQPEYHSEIQAAQNGTTNAATGGEKDELFDEAKRIVLTSRTASTSYLQR
                     RLGLGYTRAARIIDQLEDAGVIGPQQGAKPREILMPNPVGDYAEAVPAEEQEAGTGDD
                     R"
     gene            complement(2283..3134)
                     /locus_tag="PLY30_01465"
     CDS             complement(2283..3134)
                     /locus_tag="PLY30_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPW64800.1"
                     /translation="MKKERINEIWGVFFLLLGLFTFGALFFFHHEDIAFYTSQPAIPV
                     RNYTGVIGAYVAFGLSLVFGYSAFVIPIVFLLWAECFFLQKVPERKLFKFVGLGIALF
                     SLATIVTITVPPESRFTAGGAFGYMAGNHLVKLFGRLGSYIMAGAALLLSVLLATDFL
                     IYPIFKGFVERVRAIMKKIPKKVAKAQKEAGKLAASLNRPESKKSEKEKAREREKPEV
                     KLPPEFSNPTKIRKYQPMSEDAAKKAKDERPEPAREETKKKPAAPQAVSAGETKPASE
                     SDDSFKA"
     gene            complement(3241..>3378)
                     /locus_tag="PLY30_01470"
     CDS             complement(3241..>3378)
                     /locus_tag="PLY30_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPW64801.1"
                     /translation="EPFTVTRGMRIAQMVIARVVKAKLEVADNLDETRRGAGGFGHTG
                     Q"
BASE COUNT          660 a          983 c          986 g          749 t
ORIGIN      
        1 acccgttctc tgaagcataa gcggcggatt tcccgagcct catgtcaaaa caaaggccgc
       61 accgctcccc gcgttcgggc tccagttcat gacctttgac cgtttcgaac cagagtcccc
      121 cgtccccgtc cctgtcaatg aagggtatac cgcgttttcc ggcataatgg atcacttcgt
      181 tcttgcgaag gttgtattcc tcgcgcgggt agatgttcgg attgtaaaaa aagaccgtcg
      241 gctcaagacc gaccgcaagc atccgatgga ggatcgacgc gcaacagggc gcgcagcaga
      301 cgtgcaggag aacctttttt tgcgggtgaa ggaaccccgg aatcttcaag gcactgatat
      361 cgatggggtc agacatcgga aattccgttt acgtatatga gatcggtgtt tcggtgtcac
      421 gggtcctgtt tccggagctt tcaaggttcg aaagagagat cgattcttcc ctcaacatca
      481 cggggatgcc ctgacggacg gcatagagat accggcggtc gtcgcgcaca agaccgccgt
      541 ctgtcagccg cgtgaccttc tgtccgccgc gcgtccggag agtgcctttc gcgatcgcgc
      601 cgttgacttt cgtgatgagt attggatcga ccggtgtcag tggctgttgg gtctcgggac
      661 agcaaaggat ggtcaggagt tcgtcagtga tcatggaggt tattataggg tccggaaggg
      721 atttcttaaa cggtttcttg gcagtccgtc cgggactcac cggtcgtctc cggtcccggc
      781 ttcctgttct tccgccggca cggcttccgc gtaatccccc acagggttcg gcatcaggat
      841 ctcgcgcggc tttgctccct gctggggacc gatcacaccg gcgtcttcca gctgatcgat
      901 aatccgcgcg gcgcgtgtat atcccagtcc gagacggcgc tggagatagg aagtcgaggc
      961 cgtgcgactg gtcagaacga tccgcttcgc ctcatcaaaa agctcgtcct tctcgccgcc
     1021 ggtcgcggca ttggtggtgc cgttctgggc ggcctgtatc tcgctatggt attcgggttg
     1081 tccctgcttc gagacaaaac cgacgatcct gttgatttca tcgtccacga tgagcggcgc
     1141 ctggccgcgg atcatattgt cactgccggg ctggatgaag agcatgtctc cgcgaccgag
     1201 aagtttgtcg gcccccttgt tgtcgaggat ggtccgtgaa tccacgacgg aggcgacctt
     1261 gaacgcgacg cgggccggaa aattagcctt gatcacgccg gtgatgacat ccacggaggg
     1321 acgctgcgtc gcgaggatca gatggatgcc gaccgcgcgg gaaagctgcg caaggcgcgt
     1381 gatcgccgtc tcgaccttgt cctgagcgac cagcatgagg tcggcaagct cgtcgatgat
     1441 aaccacgata tagggaagcg acgcgggaac cacgttctcg gaatccttgg gcgcctccgc
     1501 aagctgcttt ttgtcctctt cggagatctc gcgttcgttg aaggtctgga tgttccggac
     1561 gccgaccgcc gcgaacagac gatagcgccg ctccatctcc tggaccaccc agttcagggt
     1621 gttcgcggcc ttgcggacat ccgtcacgac cggcgagagc atgtgcggaa tcttgttgta
     1681 caccgcgagc tcgaccatct tcgggtcaac catcaccagt ttgagctggg acggtgtgca
     1741 atggtacagg aagcccgcga tgaccgagtt gatgcagacg gtcttgccgc tgcccgtcgt
     1801 cccggcgatc aggatatgcg gcatcgaggc aaggtcggcg atcatcggtt tgcccgccgt
     1861 gtccttcccg aggagaagcg gcagtttctg actgctcgat ttaaaatcgg gcgcttcgat
     1921 catttcccgg atcacgatgg gcctcgaaac ggcgttcggc acctcgatcc cgatcgtctg
     1981 tttaccggga atcgggatga tcagacggat gctcgtcgcc ttgagcgcca tcgcgaggtc
     2041 gtcctcgtag gccgagatcg agttcgcgcg gacccccggc gccggaagaa cctcgtaaag
     2101 cgtgatcttc ggcccttgct ccacttcgac aaccttcacg tcgatatcaa attctttcag
     2161 cgtctcttcg atagctttcg agtttgcggc gaggtcgtcc ccttcgatct tttcggcctt
     2221 cggcatcctc aaaaggtcgg tcggcggaaa ttcataaggc cggtcggacg cgatcgaccc
     2281 gatcacgcct tgaacgagtc atcactttcc gaggcgggct tcgtttctcc ggcagacacg
     2341 gcctgcggtg ccgccggctt ctttttcgtt tcttcccttg caggctccgg ccgttcgtcc
     2401 ttcgcctttt tggccgcatc ctcagacatc ggctggtact tcctgatctt ggtcggattc
     2461 gagaattccg gaggcagttt gacctccggt ttttcccttt ccctcgcttt ttccttttcc
     2521 gatttcttcg actccggacg gttcagggag gcggccagtt tcccggcttc tttctgggcc
     2581 ttcgcgacct ttttcggtat tttcttcatg atcgcgcgga cacgctcgac aaaaccctta
     2641 aagatcggat agatcagaaa gtccgtcgcg agcaggaccg aaagaaggag tgcggcgccg
     2701 gccatgatgt aactgccgag cctcccgaaa agcttcacga gatggttccc tgccatgtag
     2761 ccgaacgccc cgcccgctgt gaagcgggat tccgggggaa cggtgatcgt cacgatggtc
     2821 gcaagcgaga acagcgcaat gccgagtccg acgaatttaa aaagttttcg ttccgggacc
     2881 ttctggagga aaaagcactc ggcccacagg aggaacacta tcggaatcac gaaggcgctg
     2941 tagccgaaca cgagagaaag accgaaggcg acgtatgccc cgatcacacc ggtgtaattc
     3001 cggacgggga tggcgggttg cgaggtgtag aaagcgatat cctcatgatg gaagaaaaaa
     3061 agcgccccga acgtgaacag gcccagcagg agaaaaaaaa caccccagat ctcgttgatt
     3121 cgttcttttt tcatgacttt cagcggttag agccaggcgg ccagcgtaaa gaataataag
     3181 gacaaacagc cggattcaaa acggcaacga acgcctggcg ttttacgctg tacgccaggt
     3241 tcattggccg gtatggccga acccgccggc gccccggcgg gtctcgtcca ggttatcggc
     3301 cacctcgagc ttcgccttta ccacacgcgc gatcaccatc tgcgcgatgc gcataccgcg
     3361 cgtgactgtg aacggctc
//
LOCUS       DAOOBX010000065         3371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_31349_length_3371_cov_3.112183, whole genome shotgun sequence.
ACCESSION   DAOOBX010000065 DAOOBX010000000
VERSION     DAOOBX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31349_length_3371_cov_3.112183"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLY30_01475"
     CDS             complement(<1..191)
                     /locus_tag="PLY30_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64802.1"
                     /translation="MTKPADPSATEGQRSGRFGAGDEKFDVISYEELFGNKNPVELEI
                     GCGKGKFLTERARENPDTN"
     gene            complement(218..1282)
                     /locus_tag="PLY30_01480"
     CDS             complement(218..1282)
                     /locus_tag="PLY30_01480"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPW64803.1"
                     /translation="MLTVCDTSRIPKIKPACALFGTCGGCKYQDIPYEDELVLKAGAL
                     RALLTDALEGLDPEVIESVVPSPDFYHYRNRLDLTLRKDRDGGWKMGFMLENTHRLEE
                     VTACPIARHEINDFLPELRKEAVSKAEAGEYRNANLTVRVGEAGKVAWGGIGRRSLRM
                     PQEDYFSVTLRGRRIFYSLDTFFQANLSILGKIVGHLADNIRWTRETVFLDLYSGVGL
                     FGIALADLAKEIVMIESGHDSVRLARHNIEYHKLGHARILEGQVEQYLPEVLKETAGR
                     DRVALIDPPRTGLCPSVIETLNAAEELASLFYLSCSPVSLARDLKKLTAGTWTVGKIT
                     PFDFFPRTRHLETLVCLGRR"
     gene            1412..1768
                     /gene="folB"
                     /locus_tag="PLY30_01485"
     CDS             1412..1768
                     /gene="folB"
                     /locus_tag="PLY30_01485"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPW64804.1"
                     /translation="MDGKIFIQGLETNCIIGTLPGERRRKQKIVVDLGFPAPVKKPAK
                     TDDLRDALNYKAIAERVASFVSGSRFYLIETLAERLAALLLKEFSLGSITLRVSKPKA
                     LGNARNVGVEIVRKSA"
     gene            complement(1765..2070)
                     /locus_tag="PLY30_01490"
     CDS             complement(1765..2070)
                     /locus_tag="PLY30_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64805.1"
                     /translation="MKKLATMLILTTLIAVGPLPRVFASDARNMAYIQRGAGRVLGST
                     LAIPAAILQDSGRVMFPFGIVTGVIRGTARMVSGIIGGTMDMVCGGAPYAKYAALAL"
     gene            2122..3054
                     /gene="rfaD"
                     /locus_tag="PLY30_01495"
     CDS             2122..3054
                     /gene="rfaD"
                     /locus_tag="PLY30_01495"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HPW64806.1"
                     /translation="MFPHMKNVLVTGGSGFIGSNLVMELQSRFPEAFITVLEDFRSSS
                     FKNLLGFKGDILAFNCADKSWMTWARGKKLDIIYHMASITDTTIHDQKLMMNDNVEGF
                     RNILELGVEKKAPVIYASSAATYGTIDRPIKESDAGRANNIYGYSKWIMDQLARSYYG
                     KTRVVGLRFFNVFGPKEDFKGHAASMIYQLCLQMMEGKRPRIFKWGEQKRDHIYVKDV
                     VSAALMAHTSKQSTVMNLGTGRATSFNEIVDALNEALGTSLKPDYFDNPYASFYQNFT
                     QADMSHHEAMTGFRCKFATREGILDYVRNHLAKK"
     gene            3142..>3371
                     /locus_tag="PLY30_01500"
     CDS             3142..>3371
                     /locus_tag="PLY30_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64807.1"
                     /translation="MKREQFISVFFIAALVFIVYLVIQIFSPFISAMFWSAVLAFALF
                     PAYAPFQKRFPKHGTLTATIMTFLVFVLIILPL"
BASE COUNT          743 a         1052 c          863 g          713 t
ORIGIN      
        1 aaattcgtgt ccggattctc ccgcgcacgt tccgtgagga atttcccctt tccgcagccg
       61 atctcgagct ccacggggtt tttgttcccg aaaagttctt cataagaaat aacgtcgaac
      121 ttctcgtccc cggcaccgaa ccgcccggaa cgttgtccct ccgttgcgga gggatcagcc
      181 ggcttcgtca cccgcccctc atccggggca tccttcgtta ccggcggcca aggcatacca
      241 gcgtctccaa atgacgggtc ctcgggaaga aatcgaacgg cgtgatcttc ccgaccgtcc
      301 acgtcccagc cgtgagtttt tttagatcgc gggcgagcga taccggtgaa caagaaagat
      361 aaaaaaggga tgcaagttcc tccgcggcat tgagcgtttc gatcacggag ggacaaagcc
      421 ccgtgcgcgg gggatcaatc aacgcgacgc ggtccctgcc ggcggtctcc ttcaggacct
      481 ccggcagata ttgttcaacc tgcccttcga ggatcctcgc gtgtccaagt ttatggtatt
      541 caatattatg acgggccaac cgcaccgaat cgtgcccgga ctcgatcatc acgatctctt
      601 tcgcgaggtc cgccagggcg atcccgaaaa gcccgacacc ggaatacagg tcgagaaaga
      661 ccgtctcccg cgtccaccgg atattgtccg cgagatgccc gacgatcttc ccgaggatcg
      721 aaaggttcgc ctggaaaaac gtgtcgagcg aataaaagat cctgcggccg cggagagtca
      781 ctgaaaaata atcctcttgc ggcatcctga gcgacctccg gccgatacca ccccaggcga
      841 ccttcccggc ttcaccgacg cggaccgtga gattcgcgtt ccggtattcc cccgcttccg
      901 ctttcgagac ggcttccttt cgaagctccg gcaaaaaatc attgatctcg tggcgcgcga
      961 tcgggcaggc ggtcacctcc tcgagacggt gcgtgttctc gagcatgaat cccattttcc
     1021 agccaccgtc ccggtccttg cgaagcgtga ggtccaggcg attccggtaa tgatagaaat
     1081 ccggtgaagg cacgacggat tcaatgacct cgggatcgag gccttccaag gcgtccgtca
     1141 gaagcgcgcg gagagccccg gctttcagga caagttcatc ctcatacggg atatcctggt
     1201 acttgcaacc accgcaggtc ccgaaaaggg cacaggccgg cttgatcttt ggaatacgtg
     1261 aagtgtcaca aacggtcagc atgatggcgc ctagtttacg gacaagcccc ggcgccgtcg
     1321 agggttgttt tcaccccctc caccccacgc tgtcgaataa attcaaccgc atgggttgat
     1381 ggccgtcccg aacaacgtta tactgcccgc catggacgga aaaatcttca tccagggtct
     1441 cgaaacaaac tgtattatcg gcacgctccc cggagaacgc cgccggaaac aaaagatcgt
     1501 ggtcgacctc gggttccctg ccccggttaa aaaacccgcg aagaccgatg accttcgcga
     1561 tgcccttaat tacaaagcga tcgcggaacg ggttgcgagc ttcgtctcag gaagccgttt
     1621 ttacctcatt gagaccctcg cagaacgcct tgcggccctt ctcctgaagg aattctctct
     1681 cggaagcatt actctcaggg tttccaaacc gaaggctctc gggaacgcgc gaaatgtcgg
     1741 ggtggaaatc gtaaggaaat cggcttaaag cgcgagagcg gcgtacttgg cgtaaggggc
     1801 cccgccacat accatatcca tcgtcccgcc gatgatcccg ctcaccattc tcgcggtccc
     1861 gcggatcacg cccgtcacga tcccgaacgg gaacatcact cggccggaat cctggaggat
     1921 cgccgccgga atggccagcg tactgccgag cacccggccg gccccccgct ggatgtaggc
     1981 catattcctc gcgtcggaag caaacacccg cgggagtggc cccactgcaa tcagcgtcgt
     2041 cagaatcagc atcgtcgcaa gcttcttcat ggcaggtgta tcggcattta aagtattgaa
     2101 ctttcgggga atttggatac catgttcccc catatgaaga acgttctcgt cacgggcgga
     2161 tccgggttca tcggctccaa cctcgtcatg gagctccaga gcagattccc ggaggccttt
     2221 atcacggtcc ttgaggactt ccgcagttcc tcgttcaaga acctcctcgg ttttaaagga
     2281 gatatcctgg cctttaactg cgccgataag tcgtggatga cgtgggccag agggaaaaag
     2341 ctcgacatca tttatcatat ggcatccatt acggatacca ccatccacga ccagaagctg
     2401 atgatgaatg acaatgtcga gggattccgc aatatccttg aactcggcgt cgaaaagaaa
     2461 gccccggtca tctacgcctc ctccgccgcc acttacggca cgatcgatcg cccgatcaag
     2521 gaatccgacg ccggccgggc gaacaatatc tacggctatt ccaagtggat catggatcag
     2581 cttgcacggt cctactacgg aaagaccagg gtcgtgggac tccgtttctt taacgtcttc
     2641 ggcccgaaag aggatttcaa aggccacgcg gccagcatga tctaccagct ctgtctccaa
     2701 atgatggaag gcaagcgccc cagaatcttc aagtggggcg aacagaaacg cgaccatatc
     2761 tatgtaaaag acgtcgtgtc ggccgctctc atggcccaca cgtcgaaaca atcgacggtc
     2821 atgaacctcg gcaccggccg cgcgacctcc tttaacgaga tcgtggatgc cctgaacgaa
     2881 gcgctcggca ccagtttgaa gccggattat ttcgacaatc cctacgcatc cttctaccag
     2941 aatttcaccc aggccgacat gagccaccac gaggccatga ccgggttccg ctgtaaattc
     3001 gcgacccgcg aaggcatcct cgattacgtc cgcaatcacc tcgccaaaaa ataaccggtt
     3061 ccgcctttac gctcttccag cgcgtcccgg gccacaaaaa gcggattgcc ctctttttgc
     3121 gcgctatgct agactagccc catgaaacgc gaacagttca tttccgtttt cttcattgcg
     3181 gcgcttgttt tcatcgttta cctcgtcatc cagatctttt cgcctttcat ctccgccatg
     3241 ttctggtccg cggtgctggc cttcgcgctc ttccctgcgt acgccccttt ccagaaacgc
     3301 tttccgaagc acgggactct gaccgcgacc atcatgacgt tcctcgtctt tgtcctgatt
     3361 atcctgcctc t
//
LOCUS       DAOOBX010000066         3354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_31528_length_3354_cov_2.873295, whole genome shotgun sequence.
ACCESSION   DAOOBX010000066 DAOOBX010000000
VERSION     DAOOBX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31528_length_3354_cov_2.873295"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PLY30_01505"
     CDS             complement(<1..755)
                     /locus_tag="PLY30_01505"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPW64808.1"
                     /translation="MKGMKRLAILGSTGSVGENALRIVRIHPDRFRVDALAVKKSVAR
                     LYEQAVEFRPKIVCIYDGVPAGAWLSRFKKLGVRVVTGDKGLEEVATFPSVRQVVCAM
                     VGAAGLKPIFAAVRARKMLAVANKEPLVMAGKLLLEEAAKHGVAVLPVDSEHSALWQC
                     LEGRDPCSVKKLILTSSGGPFRNVKGSLARITPAQALAHPKWKMGPKITVDSATMMNK
                     GLEAIEAANLFHVEAKKIEVVIHPEAIVHALVE"
     gene            complement(845..1642)
                     /locus_tag="PLY30_01510"
     CDS             complement(845..1642)
                     /locus_tag="PLY30_01510"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPW64809.1"
                     /translation="MSALAKRIGMSFILVPMCIVVIFYAPHWVFFIVVEGFILLALNE
                     FFSIAENKGLIIHRGFGLCFGALLPFAAYSSLQLPLVLGACVALFIFLFNRRSPDRTI
                     TSVAVSVFGIVYVAWFFSYLIKLRLLPDGAMWVFYTILIVKAGDAGAYFVGSHLGRVK
                     LLPLVSPNKSVEGAIGQMITTVALSMAALFFIDIAWYHMLLLGLGVGGLAIFGDLAES
                     LIKRDAGVKDSGQLPGLGGVLDVIDSLLFTVPFVYFYLSFVLGLERM"
     gene            complement(1773..2501)
                     /locus_tag="PLY30_01515"
     CDS             complement(1773..2501)
                     /locus_tag="PLY30_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPW64810.1"
                     /translation="MPNEIPQHVAIIMDGNGRWAKRQGFPRIKGHEEGVRRVEEVVHA
                     AQVMGVKYLTLYTFSRENWRRPKEEVDFLMNLLSVYLDGKISVLIRDNVVLNMIGELN
                     DLPEALQKKLLDARRASEKNTGLTLTLALSYSSRAEIARACRKIAEKARSGELDPKEI
                     SEATVSEYLLTAGMPDPELLIRTSGEMRISNFLLWQISYSELYVTPKLWPEFTPDEFS
                     KAIEDYRGRERRFGNTTAAKGLPR"
     gene            complement(2513..>3354)
                     /locus_tag="PLY30_01520"
     CDS             complement(2513..>3354)
                     /locus_tag="PLY30_01520"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HPW64811.1"
                     /translation="IRVCDLKSMSYLRQRLSDVVRERNAVLTKVYKHKPISFKKTFAY
                     CKKIRSRLLKLAIDTPKYLYEASRAGKDILFEGAQGTLLDVDHGTYPFVTSSNATVGG
                     AITGTGIAPTFIDRILGVVKAYTTRVGEGPFPSQFPAGLMEIVQKKGDEFGSTTGRPR
                     RCGWFDAVIAGHAVRINGLDAMAIMKLDVLSGLKELKIATAYRVNGKVTRDYPHAINE
                     FSAAKPIYETMKGWDEDITGCKKWSDLPVNARKYLKRLEQLTGTPIKIVSVGSQRHQT
                     IFL"
BASE COUNT          716 a         1024 c          932 g          682 t
ORIGIN      
        1 aattcgacca gggcgtgcac gatcgcttcg ggatggatca cgacctcaat cttcttcgct
       61 tcgacgtgaa acaggttcgc ggcctcgatc gcctcgagcc ccttgttcat catggtcgcc
      121 gagtccaccg tgatcttcgg gcccattttc catttcggat gagcgagcgc ctgcgcgggc
      181 gtgatcctcg cgagcgagcc tttcacgttc cggaacggcc cgcctgaaga ggtcaaaatg
      241 agctttttaa ccgaacaggg gtcccggcct tccagacatt gccagagcgc cgaatgttca
      301 ctgtcgacgg gaagcaccgc cacgccgtgt ttcgccgctt cttccagaag aagctttccg
      361 gccatcacaa gaggttcctt gttcgcgacg gcgagcattt tccgggcccg gaccgccgca
      421 aagatcggtt tgagacccgc cgcccccacc atcgcgcaga ccacctgccg gacggacgga
      481 aacgtcgcga cctcttcgag acccttgtcc cccgtcacga cccggacgcc gagtttttta
      541 aatcgggaaa gccaggcccc ggcgggcaca ccgtcataaa tgcaaacgat tttcggcctg
      601 aattcaacag cctgttcata gagccgcgcg acgctttttt tgacggcaag ggcgtccacg
      661 cggaaccggt cgggatggat acggacaatc cggagcgcgt tctccccgac cgagccggtc
      721 gaaccgagga tggcaagacg cttcatccct ttcatttccc gatcttttcg tgatttcccg
      781 tgagagatca accgaaggtc tccgttctgg attttgccgt tccgatcact gcccgtccgg
      841 atttctacat ccgctcaagg ccgaggacaa agcttaaata gaaataaacg aacggcacgg
      901 taaagagaag gctgtcgatc acatccagca caccgccgag accgggcaat tgtcccgaat
      961 ctttcacccc ggcatcccgt ttaatcaggc tctcggccag gtccccgaag atcgcgagcc
     1021 cgcccacgcc gagccccagg agcagcatgt ggtaccacgc gatatcgatg aaaaaaagcg
     1081 ccgccatcga aagcgccacg gtcgtaatca tctgcccgat cgcgccttcg accgacttgt
     1141 tcgggctcac gagcggcagg agcttcaccc ggcccagatg gcttccgaca aaataggctc
     1201 cggcgtctcc cgccttcacg atcaggatcg tgtagaaaac ccacatggct ccatccggca
     1261 aaagccgcag cttgatcaga taggaaaaaa accacgccac ataaacgatc ccgaacacgc
     1321 tgaccgcgac gctcgtgatc gtgcgatcgg gagaacgccg gttgaaaaga aagataaaca
     1381 gggcaacgca agccccgagc acaagcggaa gctgaagcga cgaataagcc gcgaacggaa
     1441 gaagcgcgcc gaaacacaac ccgaatcccc ggtggatgat gagccccttg ttctcggcga
     1501 tcgaaaagaa ttcgttcagc gcgagcagaa tgaacccctc gaccacgatg aagaacaccc
     1561 agtgcggcgc gtaaaaaatc acgacaatgc acatcggcac caggataaaa gacatgccaa
     1621 tccgtttcgc gagcgcgctc atgcttgctc cgtcagagac aggtcctgct caagcgaagc
     1681 gggaccgcgt ttgtatcgtt tgtatgatga atggctcatt cgaaacccat ccttttccat
     1741 aatccgatcg tgacccgtct gattcatgat ccctaacggg gtaaaccttt cgcagccgtc
     1801 gtgttgccga accggcgttc acggccgcga taatcctcga tcgcttttga gaactcatcc
     1861 ggcgtgaatt cgggccagag tttcggcgtc acatagagct cgctgtagga gatctgccag
     1921 agaaggaaat tgctgatcct catctccccg ctcgtccgga tcaggagttc ggggtccggc
     1981 atgcccgcgg tcaggagata ttcgctgacc gtggcctcgc tgatctcctt gggatcgagt
     2041 tcgccgctcc gcgctttttc cgcgatcttg cggcaggccc gcgcaatctc ggcgcgcgac
     2101 gaatagctga gcgcaagcgt cagggtgagc cccgtgttct tttcggaggc ccggcgcgcg
     2161 tccaggagtt ttttctgaag cgcctccggc agatcgttca gttcgccgat catgttcaga
     2221 accacattgt cccggatcag gacgctgatc ttgccgtcga gatacacgga aaggaggttc
     2281 atgagaaaat cgacctcctc tttggggcgg cgccagttct ccctggaaaa ggtgtagagc
     2341 gtaaggtact tgacgcccat aacctgggcg gcgtggacga cctcttcgac gcgccggaca
     2401 ccttcttcat gacctttgat gcgggggaaa ccctgccgtt tcgcccatcg accgttgccg
     2461 tccatgatga tcgcaacgtg ctgggggatt tcgttcggca tcgggcaggc ccttacagga
     2521 aaatggtctg gtgacgctga cttccaacgg acacgatctt gataggtgtc cccgtcagct
     2581 gttcgagtct cttgagatat ttgcgggcgt tcacgggaag gtcggaccat ttcttgcatc
     2641 cggtgatgtc ctcgtcccag cccttcatgg tctcgtagat cggcttcgcc gcggagaact
     2701 cgttgatcgc gtgcgggtag tcgcgcgtga ccttgccgtt cacccggtag gccgtcgcga
     2761 tcttgagctc cttcagtccc gacaggacat ccagcttcat gatcgccatc gcatcgaggc
     2821 cgttgatccg gaccgcgtgg cccgcgatca cggcatcgaa ccatccgcag cggcgcgggc
     2881 ggccggttgt ggaaccgaac tcgtcaccct ttttctgaac gatctccatg agcccggcgg
     2941 ggaactgcga cgggaacggc ccctcgccca cgcgcgttgt gtacgccttc accacgccga
     3001 gaatccggtc aatgaaggtc ggggcaatgc cggtccccgt gatcgcaccg ccgacggtcg
     3061 cgtttgagga ggtcacgaac ggataggtcc cgtgatcgac atcgagaaga gtcccctggg
     3121 caccttcgaa gagaatatcc tttcccgccc tggacgcctc ataaagatat ttcggggtat
     3181 cgatcgcaag tttgagaagc cgcgaacgga tctttttgca gtaagcaaag gtctttttaa
     3241 acgaaatggg tttgtgcttg tagaccttcg tgagaaccgc gtttcgttcc ctgacaacat
     3301 ccgaaagccg ctggcggaga tagctcatgg acttgagatc gcagacacgg atac
//
LOCUS       DAOOBX010000067         3335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_31757_length_3335_cov_3.038720, whole genome shotgun sequence.
ACCESSION   DAOOBX010000067 DAOOBX010000000
VERSION     DAOOBX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31757_length_3335_cov_3.038720"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLY30_01525"
     CDS             <1..727
                     /locus_tag="PLY30_01525"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPW64812.1"
                     /translation="KLVSVVHVSNALGTLNPVEEIIRLAHEKGAKVLIDGAQAVAHEA
                     VNVRDLDADFYVLSGHKLFAPTGIGVLYGKAELLDRMPPYQGGGDMIRSVTFEATTYN
                     KIPYKFEAGTPHIAGAIGLGVAVDYLRGIGLERIAAYEKELLAYGTKKLLAIPGLRLI
                     GTAKRKAAVLSFTLGEAHAHDIGTILDRAGIAIRAGHHCAMPLMQRFGVSATTRVSLA
                     FYNTREEIDRLSDALREVVEVFK"
     gene            724..1179
                     /locus_tag="PLY30_01530"
     CDS             724..1179
                     /locus_tag="PLY30_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014148483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HPW64813.1"
                     /translation="MNPDLRDLYQQMIMDHNKNPMNFREIRDATSRAEGFNPLCGDHL
                     HVYLKMNGDRIEDISFEGAGCAISKASGSMMTAALKGKTRAEAEGLFAEVRAMLSREP
                     GMPVNAEKLGKLAVFAGVCEFPLRVKCASLAWHTMMEAIAPTGKQVSTE"
     gene            1226..1555
                     /locus_tag="PLY30_01535"
     CDS             1226..1555
                     /locus_tag="PLY30_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HPW64814.1"
                     /translation="MENGKTVEENIWDELRLCFDYEIPVNIVELGLVYSVRVNGLPEG
                     GGREAVIEMTFTSALCPMADIIKKDVERRVSSVSGIKKVTLRIVWDPPWDPSRMSEAA
                     KLQLGLL"
     gene            complement(1636..2916)
                     /locus_tag="PLY30_01540"
     CDS             complement(1636..2916)
                     /locus_tag="PLY30_01540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HPW64815.1"
                     /translation="MLIFQRIIGFFLRKKWLGWLLVFALFYGTCISTVYRGAIGHKQR
                     TDLTVYIKAAEMVRIGNPGHIYGIETDRHWHYVYSPFLAVTLAPVSHLPFVILVIANY
                     LLALACLFATLFLSRTFTSEGPEATWKIIFAAILCLPVILNTLTRGQLGTVMLFFQAI
                     VLFCYMRNLKVLAGVLLALAVSLKTSPLAALLFFFLFQKEWKVLLAAAAGFAVFLILY
                     PSLSIGFRTNWNLLMIWKGLMAEGSSIQAYKNYLWPELFTPFAGDNQSFYAVLIRLVW
                     RSDSDFIAHPSNWVRWISMAAGAVAMALIFLKRSRPDRRAVSPEKLMAEFSLYPAWML
                     VFSPVTQIHHYTSLYFLFLAALFLSGKENPRSRFLTVGRAFSAVLFLLGLIIDPLAYL
                     GLPMWGTLALGGIVLFCLDGKQDGHAGRAQSPGP"
     gene            3033..>3335
                     /locus_tag="PLY30_01545"
     CDS             3033..>3335
                     /locus_tag="PLY30_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64816.1"
                     /translation="MSAVCSRKRLYESRVIQEDIAQLAKNLKHYHKYFEGSAFLVTGA
                     GGFLGNYMTLLFRHMNQHHLRHRKLRAILLDNFITGYEQQIISDENITFWRHNVIEP"
BASE COUNT          763 a          909 c         1042 g          621 t
ORIGIN      
        1 gaaacttgtt tcggtggtcc atgtttcgaa cgcgctcggc accctcaacc cggtggagga
       61 gatcatccgt cttgcccatg agaagggcgc gaaggtcctg atcgacgggg cgcaggccgt
      121 ggcgcacgag gccgtgaatg tccgcgacct ggacgccgat ttctacgtgc tctccggaca
      181 caaattattc gcgccgacgg ggatcggcgt actgtacggg aaggcggaac tgctcgaccg
      241 gatgccgccg taccagggcg gcggggatat gatccgttcc gtgacctttg aagcgaccac
      301 ctacaacaag attccctaca agttcgaggc ggggacgccg catatcgccg gcgcgatcgg
      361 gctcggcgtc gccgtggatt atctccgggg gatcgggctg gagcggatcg ccgcgtatga
      421 aaaggagctc ctcgcgtacg ggaccaaaaa gcttctcgcg atcccgggac tccggttgat
      481 cggaacggcg aagcgcaagg ccgccgtgct ctctttcacg ctcggcgagg cccacgccca
      541 tgatatcgga acgattctcg accgcgcggg cattgcgatc cgcgcgggcc atcactgcgc
      601 gatgccgctc atgcagcggt tcggagtatc cgcgacgaca cgcgtctcgc tggcctttta
      661 caacacgcgg gaagagatcg accgtctgtc ggacgctttg agagaagttg tggaggtatt
      721 caaatgaacc ccgatctccg ggacctctac cagcagatga ttatggacca caacaagaac
      781 ccgatgaact tccgggaaat ccgggatgcc acgtccaggg cggaggggtt caaccccctt
      841 tgcggggacc atctccacgt ttatctcaag atgaacggcg accggatcga ggatatcagt
      901 ttcgaagggg cgggttgcgc gatctcgaag gcatcggggt ccatgatgac ggcggccctg
      961 aaagggaaaa cgcgcgcgga ggcggaaggc cttttcgcgg aggtccgggc catgctttcc
     1021 cgtgaacccg gcatgccggt gaacgcggaa aaactcggga agttggccgt ttttgccggt
     1081 gtctgcgaat tcccgttgcg cgtcaaatgc gcgtcgctgg cctggcatac aatgatggaa
     1141 gcgatcgctc cgacgggaaa gcaggtctcg acggaataag atcgggaacc gttccgggtg
     1201 ccagatcgtt atctggatgg gtgttatgga aaatggaaag accgtagaag aaaatatctg
     1261 ggacgagctc cggctctgct tcgattacga gattccggtt aacatcgtgg agctcgggct
     1321 tgtttattcc gtcagggtga atggcctgcc cgagggggga ggccgggaag ccgtcatcga
     1381 aatgaccttc acgagcgccc tgtgcccgat ggcggacatc atcaaaaagg atgtcgaacg
     1441 gcgcgtcagt tccgtttccg gaatcaaaaa agtcaccctt cggatcgtat gggatccgcc
     1501 ctgggaccca tcccgcatgt ccgaggcggc gaagcttcaa ttgggtttgc tgtgaagagg
     1561 aagcggcgtt gtcccgcgcc cgcgatccgc atgaaatcct gaaggataag ggtcgcgaag
     1621 ctgccgcccg gttatttatg gaccgggaga ttgcgcccgc ccggcgtgcc catcctgttt
     1681 cccgtccagg caaaagagga cgatcccgcc cagcgccaag gttccccaca tgggaaggcc
     1741 gagatacgcg agcggatcga tgataagacc caacaagaaa aggaccgccg aaaaggcgcg
     1801 acccaccgtc aagaatcggg aacggggatt ttcttttccg ctcaggaaaa gagccgccag
     1861 gaagaggaaa taaagcgacg tgtaatggtg gatctgggtg accggcgaaa acacgagcat
     1921 ccatgccgga taaagggaga attccgccat gagcttttcc ggagaaaccg cgcggcgatc
     1981 cggccgggat cttttcagga aaatcagcgc catcgcgacg gccccggccg ccatcgagat
     2041 ccagcggacc cagtttgagg gatgcgcgat aaaatcggag tcgctccgcc agacgaggcg
     2101 gatgagaacg gcgtaaaacg actggttgtc gcccgcgaac ggcgtgaaca gttcgggcca
     2161 gagatagttc ttgtacgcct ggatcgaaga tccctccgcc atgagccctt tccagatcat
     2221 aaggaggttc cagttcgtcc ggaacccgat gctgagcgaa gggtagagga taagaaagac
     2281 cgcgaatccc gcggccgccg cgaggaggac cttccattcc ttttggaaca ggaaaaaaaa
     2341 gaggagggcc gcgagcgggc tcgtcttgag gctgaccgcg agcgccagca ggacccccgc
     2401 caggaccttg aggttcctca tgtaacaaaa aaggacaatg gcctggaaaa agagcatcac
     2461 ggtgccgagt tgtccccggg taagcgtgtt gaggatgacc gggaggcaga ggatggcggc
     2521 aaaaatgatc ttccaggtgg cctccggacc ttcggacgtg aatgtcctgg acaggaacag
     2581 cgtcgcgaaa aggcacgcga gcgccagaag atagtttgcg atcacgagga tcacgaaagg
     2641 gaggtgggag acaggggcga gcgtgacggc aaggaacggg gaatagacat aatgccaatg
     2701 ccggtcggtt tcgatcccgt atatgtggcc gggattcccg atgcggacca tttcagccgc
     2761 cttgatgtag accgtgaggt ccgtccgctg cttgtggccg atggcgccgc ggtagaccgt
     2821 ggaaatgcag gtcccgtaaa agagggcgaa gaccagaagc cacccgagcc attttttccg
     2881 gagaaagaac ccgatgatcc gctgaaagat gagcatattt ttattcgtcc ttctacacga
     2941 tttaggctat aatctcaact cttttattcc ggccccgggg acaggttccg ttcctcttta
     3001 ccgggaaatc ccaacgcttt ggtgaggtga ccatgtcggc tgtctgctca aggaaacggc
     3061 tttatgagtc cagggtgatc caggaagaca tcgcgcagct tgcgaagaac ttgaaacatt
     3121 atcacaagta tttcgaaggc agcgccttcc tcgtgacggg cgcggggggc ttcctcggga
     3181 attacatgac cctccttttc cgtcacatga accagcacca tctccgtcac aggaagctca
     3241 gggcgatcct gctcgataac ttcatcacgg gttacgaaca gcagatcatt tcggacgaga
     3301 acatcacctt ctggcgtcat aacgtgatcg agccg
//
LOCUS       DAOOBX010000068         3311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_32051_length_3311_cov_3.871622, whole genome shotgun sequence.
ACCESSION   DAOOBX010000068 DAOOBX010000000
VERSION     DAOOBX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32051_length_3311_cov_3.871622"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /gene="secF"
                     /locus_tag="PLY30_01550"
     CDS             <1..585
                     /gene="secF"
                     /locus_tag="PLY30_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPW64817.1"
                     /translation="AQKVETAIKNFAGDKGYEILRVDHVGPSVSKDLTKKALWAVLWS
                     AIGILVYLALRFEWKFSLAAVIAIMHDTIFTFGVYALSGREINLPTIAAVLTIMGFSV
                     NDTIVTFDRVRDNIKLMRKTPFAEIVNLSINQTLSRTFLTSFTTILATLALFLFGGSS
                     INDFAFAMLVGFGVGIYSTIFIATALVVDWKAHK"
     gene            683..2332
                     /gene="recJ"
                     /locus_tag="PLY30_01555"
     CDS             683..2332
                     /gene="recJ"
                     /locus_tag="PLY30_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPW64818.1"
                     /translation="MAERIKNYSPAFLRLLAGRGLTDSAAIESFLSADLSALHDPFLM
                     KNMREVKSRLEEAIRKKERMLLHGDYDADGITGVAVMVKVLEKLGGDFLTFLPERERH
                     GYGVSEEAILKAHREGAKLVITIDCGITAREEIRLARSLGMEVIVIDHHRLPPEGLPD
                     THLILNPLQEDCVYPFKELSAAGLAFKLAQAMIGEESIALLDLVAISTISDIAPLVSE
                     NRALVRCGLAELSARRRMGVKCLAEVAKIRSQKIDTGHVGFILGPRINAAGRMSSPDT
                     ALRLLLTDSQREAMSLANILNEENRARQQQEREITKEAIAKVERTVNFNRDRIIVAAG
                     EGWHQGVIGIVASRLVEKYCRPAIVIAVEGDKGKGSGRSIKGFHLFNALQSCAGLLEE
                     FGGHEQAAGLSIRAGNLKAFREEINAYALENLDPAIMVRKVPVDLEIGFGDLTTVFLR
                     ELELLEPFGVGNPRPVFLTRGLSVKTRPRKIYGETYEIVVGDGSANYPALINEKQMFR
                     LATARPGSKYDFAYTVKTRVWNGIETLSLDAKEMKEPADPD"
     gene            complement(2329..2592)
                     /gene="rpmB"
                     /locus_tag="PLY30_01560"
     CDS             complement(2329..2592)
                     /gene="rpmB"
                     /locus_tag="PLY30_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPW64819.1"
                     /translation="MKICVICNKKPIVGNTIARRGLPKKTGGIGVKTTGISRRRFMPN
                     LQRVKIRFPNGTVRRVNVCASCLQSGKVTKAPVRRKTEVQAGA"
     gene            2724..3056
                     /locus_tag="PLY30_01565"
     CDS             2724..3056
                     /locus_tag="PLY30_01565"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HPW64820.1"
                     /translation="MSRPSTKISVKKPAKSVSQTCSKSCDKTVEFKFYAPQAKKVSVG
                     GSFNGWNPGKTPLKKDANGNWKASVVLGLGRYEYRYLVDGDWQDAQEAGERVPNAFGS
                     WNCVLQVR"
     gene            3095..>3311
                     /locus_tag="PLY30_01570"
     CDS             3095..>3311
                     /locus_tag="PLY30_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPW64821.1"
                     /translation="MKILFVTSEISPFARTGGLGDTLASLTAAVRAEGHDVTVFMPRY
                     KCVDIRRLGLEIAINYFEVIIGNTLEAT"
BASE COUNT          716 a          894 c          985 g          716 t
ORIGIN      
        1 gcccagaagg tggaaaccgc gatcaaaaac tttgccggtg acaaaggcta cgagatcctt
       61 cgtgttgatc atgtgggacc ttcggtcagc aaggacctta caaaaaaagc cctttgggcg
      121 gttttgtggt ccgcgatcgg gattctcgtt tatctggcgc tccgctttga atggaagttc
      181 tcgctggcgg ccgtgatcgc tatcatgcac gatacgatat tcacgttcgg tgtgtatgcg
      241 ctctccgggc gcgagatcaa cctgcccacg attgcggcgg ttctgacgat catgggtttt
      301 tcggtcaatg acacgattgt gacgttcgac cgcgtccgcg acaacataaa actgatgcgc
      361 aagaccccgt tcgcggaaat cgtgaacctc agcatcaacc agacgctgag ccggacgttc
      421 ctcacgagct tcacgacgat cctcgcgacc ctggcgctgt tcctctttgg cggatcgagc
      481 atcaacgact ttgcgttcgc gatgctggtc gggttcggcg tcggtatcta ttcgacgatc
      541 tttatcgcga cggcgcttgt ggtggactgg aaggcgcata aataagcgat aagctgtaag
      601 tggtaagttt ggagcattcg aagagggcag gccggtttcc cctggatgtc ggcctgtcct
      661 ctttttttgt cagggtgaag ttatggcgga acggataaaa aactattctc ctgcttttct
      721 caggcttctt gccgggcgtg ggctgacgga ttctgccgcc atcgaaagtt tcctttccgc
      781 cgatctttcc gctctgcacg atccgtttct gatgaaaaat atgcgagagg tgaaatcgcg
      841 cctcgaagag gcgatccgga aaaaggaacg gatgctcctg cacggggatt atgacgcaga
      901 tggcattacc ggcgtcgcgg tcatggtcaa ggtccttgaa aagctcggcg gtgatttcct
      961 gacgttcctt cccgaaaggg agcggcacgg ttacggcgtg agcgaagaag cgatcctcaa
     1021 ggcgcacagg gaaggcgcga aactcgttat cacgattgat tgcgggatca cggcccggga
     1081 ggaaatccgc ctcgcgcgga gcctcgggat ggaggtgatc gtgatcgacc accaccggct
     1141 gccgccggag ggacttcccg acacgcacct gatcctgaac ccgctccagg aagattgtgt
     1201 ttatcccttc aaggagcttt cggcggcggg tcttgccttc aagctcgcgc aggcgatgat
     1261 cggggaagag tcgatcgcgc tcctggacct tgttgcgatc tcgacgatca gcgatatcgc
     1321 gccgctcgtt tccgagaatc gcgcgcttgt tcgatgcggg ctcgcggaac tttctgcgcg
     1381 ccggcggatg ggggttaaat gcctggccga ggtcgcgaag atcaggagcc aaaagatcga
     1441 taccggtcat gtgggattca tcctgggccc ccggatcaac gcggccggca ggatgagttc
     1501 cccggatacc gcgctcaggc ttctcctgac ggattcgcaa cgggaggcga tgagcctcgc
     1561 gaatatcctg aacgaggaga accgggcccg gcagcagcag gaacgggaga tcacgaaaga
     1621 agcgatcgcg aaggtcgaac ggacggttaa tttcaaccgg gaccgtatca tcgttgcggc
     1681 cggagaagga tggcatcagg gcgtcatcgg gatcgtggcg tcgcggcttg tcgagaaata
     1741 ctgccggccc gcgatcgtca ttgctgtcga gggtgacaaa ggcaagggtt cagggcgctc
     1801 gatcaaagga tttcatttat tcaacgccct tcaatcctgc gccggacttc tggaggaatt
     1861 cggcggacac gaacaggcgg cggggctttc gatccgggcg gggaatctca aggcgttccg
     1921 cgaggagatc aacgcctatg cccttgagaa cctggacccc gcgatcatgg tcaggaaagt
     1981 cccggttgat ctcgagatcg gattcggaga cctcacgacg gtgtttctcc gtgaactgga
     2041 attgctggaa cctttcgggg tggggaaccc gcggccggta ttcctgacgc ggggactttc
     2101 ggtcaaaacc agacccagga aaatctacgg ggaaacttac gagatcgtgg tcggcgacgg
     2161 atcagcgaac tatcccgccc tcatcaatga aaagcaaatg ttccgactcg ccacggcacg
     2221 tccggggtcg aaatacgatt ttgcctatac cgtgaagacc agggtctgga acggcataga
     2281 aacactcagc ctggacgcga aggaaatgaa ggagccggcg gacccggatt aagcgcccgc
     2341 ctggacttcg gttttccgcc gaacgggcgc cttggtgacc ttgcccgact ggagacagct
     2401 ggcgcaaacg ttgacgcgcc gcaccgttcc gttcgggaac ctgatcttga ccctctggag
     2461 gttcggcatg aagcgacggc gggaaatgcc ggtagtcttg acaccgatac cgccggtctt
     2521 tttaggaaga ccacggcgag cgatagtatt gccgacaatc ggcttcttat tgcagatgac
     2581 gcagattttc atagaggcgg tattatatgg gccagatgct tcttggcaag ttgttttttc
     2641 tgatatatac tgcccccact tccgcgtcat ttgatctacg gaatgtgaac taaccagcca
     2701 gacaatcaga aggagtttct atcatgtcga gaccatcaac caaaatttcc gtcaaaaaac
     2761 ccgctaaatc cgtttcccag acctgttcca aatcctgcga caagaccgtc gaattcaagt
     2821 tctatgctcc gcaagcgaag aaagtttccg ttggcgggag ttttaacggc tggaatcccg
     2881 ggaaaacgcc cctgaaaaag gacgcgaacg gcaactggaa agcctccgtc gtgcttggtc
     2941 tgggccgcta tgagtaccgc tatctggtcg acggtgactg gcaggatgcc caggaggccg
     3001 gcgagcgcgt tccgaacgcc ttcggcagct ggaactgcgt tcttcaggtc cgttaagcca
     3061 tcacgaagtt ccggtacccg tcctcaaaag tcttgtgaag attctcttcg ttacgtccga
     3121 gatctccccg ttcgcacgaa cggggggatt gggggatacc ctcgcttctc tgacagccgc
     3181 cgtccgcgcg gaaggtcacg atgttacggt attcatgccc cgctacaaat gcgtcgatat
     3241 tcgcaggctg ggtctcgaga tcgcgatcaa ttatttcgag gtgataatcg gcaataccct
     3301 cgaagcgacc c
//
LOCUS       DAOOBX010000069         3306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_32114_length_3306_cov_3.097816, whole genome shotgun sequence.
ACCESSION   DAOOBX010000069 DAOOBX010000000
VERSION     DAOOBX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32114_length_3306_cov_3.097816"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3306
                     /locus_tag="PLY30_01575"
     CDS             <1..>3306
                     /locus_tag="PLY30_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64822.1"
                     /translation="VKLEVSGHDTNLDTAALKTQYRDALADTVAGTVFTKSVSGKDIN
                     YSWKSAAVEGTTYSLARSREKIYEARTGVVDGTTRVLTDDDGDIVYDIIEDFSWNFSA
                     QTYVAPLTALTEAGSLGANLEVQEQKDQYASALLDTDRATAFTRTTSGANVSYSWKSV
                     EGGKTYNLGYEVVKIHAADGARVEERAKWLFSEQSYVAKSVVEGRAANLSTAELKAQY
                     AAALSDTDSSTYYTQIISGSDLSFSWESVDGGRTYSLSSERVKVETGEEDGKPVYGYQ
                     PVKWTFSAQFRVVKDEIVHQDVEFNDDAMDGQYQSALSDTDAATSFTKSISGSDISYS
                     WKSLNNAKTYSLSRTAQKSYIPETETVNGQTRVRKDADGKVIYKTHTEVVDGRPVTVM
                     DHAVETKWSFSEQCLVTHAEVDAYGTMFRGAPGGSLPAVPAELETQELKDQLAAALAD
                     TTSSTFYTRMTSGTNTSYSWNSRDGGVSYNLGAEVSKVLQPDNTTYQPKMKWSFSTQS
                     YVLRTAVDDWVSSLPQDQRSQYEAALEDIDDSTCFTRAVSGGSVNYSWTSPDGIKTYM
                     LANEKVPAEESADGYESAKWSFSTQYRVLRAEVEGHLSALDDDQQAQYTAALADANGS
                     TVYMKTVSGTEVNYVWQNPAKTVTYSLNRTLQRLSDAAGNLLIVTPPVHKWTFASQVL
                     VEKALVSAMNVVLENDAVRAQYESALRDDTSNSTEYTRSASGDEISFSWKSKAGRKNY
                     SLVRTIAKVLQADGTTWASVARWAFSSQETIRRSDVDAFVDNLESSDQKVRYQAALAA
                     ADDGAAYTKAVSGNETTYSWKSADLVTTFTMLQSVPSPGIEKWSFSAQALVRHAEVEI
                     ALTDLEEDQRAAYEAAIADVDEKTEYMRTVSGKDVSYSWKSVDETKNYTLNRTMTRVQ
                     QQDGSFAEVYAAAFTVQCAVTKAELEEKALRVDDPEQRAQAAAVIAGILPGVTGIIRA
                     ETGSETSYSWENSRGNVTYALSFGTVWEETDAVTHDGVFLDLWQFSEQTLVLKSEVEG
                     YLSSLGSSEQEKFLSALETDTVTKATVYTKSVSGGDISYSWQNPDLTESFTLSRQPVW
                     EETDTDTHAGSFVA"
BASE COUNT          771 a          829 c         1048 g          658 t
ORIGIN      
        1 cgtgaagctt gaggtcagcg gtcatgatac gaatctggat accgcagcgc tgaaaaccca
       61 gtatcgtgac gctcttgcgg atacggttgc cgggacggtg ttcacgaaga gtgtgagcgg
      121 gaaagatatc aactacagct ggaaatccgc cgcagtggaa gggacgacgt actccctggc
      181 ccgttcccgc gagaagatct atgaggcgag gaccggtgtc gtggacggta cgactcgtgt
      241 cttgaccgat gatgacggag atatcgttta cgacattatc gaggattttt cctggaattt
      301 cagcgcgcag acgtatgtgg cgcccctcac cgcattgacc gaggcgggaa gcctcggggc
      361 caatcttgaa gtccaggagc agaaagacca gtacgcgtca gcgctgctgg atacggaccg
      421 ggccacggcg tttacgagga ccacgagcgg agcgaatgtc agttatagct ggaagtcagt
      481 cgaaggagga aagacctaca atctcggata tgaggttgtg aagatacacg cggccgacgg
      541 cgcccgggtt gaggagcggg cgaaatggtt gtttagcgag cagtcctatg tcgcgaaaag
      601 tgttgtcgaa ggccgcgccg cgaatctttc tacggcggaa ctgaaagcgc agtacgcggc
      661 cgcgctttcg gatacggata gttcgacata ctacacacag atcatcagcg ggtcagacct
      721 ttctttcagc tgggaatccg ttgacggcgg caggacctac tcactttcca gtgagcgcgt
      781 caaggtcgag accggcgaag aggacgggaa gcctgtttac ggctatcagc ctgtgaagtg
      841 gacctttagc gcccagttcc gtgtcgtcaa ggatgagatt gtgcatcagg acgtggaatt
      901 taacgatgat gcgatggacg gacagtatca gtcagctctt tcggatacgg atgctgccac
      961 gagcttcacg aaatccataa gcggcagcga tatcagttat tcctggaaga gtctgaacaa
     1021 cgcaaagacc tattcactga gcaggacggc gcagaagagt tatatccccg aaaccgagac
     1081 cgtgaacggg cagacgcgcg tgcggaaaga cgctgacggg aaagtgatct acaagacgca
     1141 tacggaggtc gtggacggcc gtcctgtcac ggtcatggat catgcggttg agacgaaatg
     1201 gagctttagc gaacagtgcc tggtgacgca cgctgaggtg gatgcttacg gtacgatgtt
     1261 ccggggcgcc cccggcgggt ctttgcccgc ggttccggcc gagcttgaga cgcaggagct
     1321 gaaagaccag cttgctgccg ctcttgctga tacaacttcc tccacgttct atacccggat
     1381 gacgagcggg acgaacacaa gctacagctg gaattccagg gatggcgggg tgtcctacaa
     1441 tctcggcgcc gaagtctcca aggtactcca gccggacaat acgacgtacc agccgaaaat
     1501 gaagtggtcg ttcagcactc agtcgtacgt tctgcggacg gcggtcgatg actgggtctc
     1561 gagcctgcct caagatcagc gctcgcaata cgaggcggct ctggaggata tcgatgactc
     1621 gacctgtttc acgcgcgcgg taagcggggg gagcgtcaat tacagctgga cgtctccgga
     1681 tgggatcaag acttacatgc tcgcgaatga aaaggtcccc gcggaagaat cagcggacgg
     1741 ctacgaatcc gcaaagtgga gtttcagcac ccagtaccgc gttctgaggg ctgaggtgga
     1801 gggccatctt tccgcgctgg atgacgatca acaggctcag tacaccgccg cgctcgcgga
     1861 tgcgaacggt tcgacggttt acatgaaaac cgttagcggg acggaagtga attatgtctg
     1921 gcagaatccc gccaaaacag ttacgtatag cctcaaccgg actcttcagc gcctttcgga
     1981 cgcggccggg aatctgctga tcgtgactcc tcccgttcat aaatggacct ttgccagcca
     2041 ggttctggtt gaaaaggcgc ttgtatccgc gatgaatgtt gttctggaga atgatgccgt
     2101 ccgcgctcag tacgagtctg cgctccggga tgatacgtca aattcgacgg agtacacccg
     2161 gtccgcgagc ggcgatgaga tttccttctc gtggaagtca aaggccggcc ggaagaacta
     2221 ttcgcttgtc cggacgatag ccaaggtcct gcaggccgat gggacgacct gggcttcggt
     2281 ggctcgctgg gctttcagct cccaggaaac gatccgcaga agcgacgtgg acgcgtttgt
     2341 tgataacctc gagagctctg atcagaaggt gcgctatcag gctgcgcttg ccgcggcgga
     2401 tgacggtgcc gcttacacaa aagcggtgag cggcaacgaa acgacttaca gctggaagag
     2461 cgcggatctt gtgacgacct ttacgatgct ccagtccgtg ccgtccccgg gaatcgaaaa
     2521 atggtccttt agcgcgcagg cgcttgtgcg gcacgccgaa gtagagatcg ctctgaccga
     2581 tctggaagag gatcagcgcg ccgcttacga ggccgcgata gcggatgttg acgagaaaac
     2641 cgagtatatg aggacggtta gcgggaagga tgtcagttat tcgtggaaga gcgtggatga
     2701 gacgaagaac tacactctga atcgcacgat gacccgcgtt cagcagcagg acggaagctt
     2761 cgcggaggtg tacgccgcgg cttttaccgt tcagtgtgcc gtgacaaaag cggagctgga
     2821 ggaaaaagcc ctgagagtgg atgatccgga gcaaagagcc caggctgcgg cggttattgc
     2881 tggaatactc cccggcgtga ccgggatcat aagggctgag acggggagcg aaacaagtta
     2941 ttcgtgggag aacagccgcg gcaacgtgac ctacgcgttg agttttggaa cggtctggga
     3001 agagacggat gcggtaacgc atgacggggt gtttttagac ctctggcagt ttagcgagca
     3061 gacccttgtg ctgaagtcgg aggtggaggg gtatttgagc tccctcggct cgtcagaaca
     3121 agaaaagttt ttgagtgccc tggaaacgga cactgtaaca aaagcaacgg tctatacgaa
     3181 gtccgtgagt ggcggtgata tcagttattc ctggcagaat ccggatctca cggaatcttt
     3241 tacgctttca cggcagccgg tctgggaaga gacggacact gacacgcacg ccggttcgtt
     3301 cgttgc
//
LOCUS       DAOOBX010000070         3243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_32938_length_3243_cov_2.900565, whole genome shotgun sequence.
ACCESSION   DAOOBX010000070 DAOOBX010000000
VERSION     DAOOBX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32938_length_3243_cov_2.900565"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PLY30_01580"
     CDS             complement(<1..1025)
                     /locus_tag="PLY30_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPW64823.1"
                     /translation="MSTESKKQPAQNKIQDFKVADLSLADWGRKEIEMAEKEMPGLMA
                     TRRKYGPKKPLKGKRIMGSLHMTIQTAVLIETLVELGADVRWCSCNIFSTQDHAAAAI
                     AKRGIPVFAWKGETLEEYWWCTLQALTFPGGKGPNLIVDDGGDATLMVHLGYDAEKNP
                     RLLDKKVETQDEIELFRVLKQCLEETPKKWRDVVAEWEGVSEETTTGVHRLYKMQEEG
                     RLLVPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVAVVLGYGDVGKGSA
                     QSLAELKARVIVTEIDPICALQASMAGYQVARLEDVLDIGDIYVTTTGNIDVITAVHM
                     SKMKDEA"
     gene            complement(1100..2287)
                     /gene="metK"
                     /locus_tag="PLY30_01585"
     CDS             complement(1100..2287)
                     /gene="metK"
                     /locus_tag="PLY30_01585"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPW64824.1"
                     /translation="MKKGNYFFTSESVSEGHPDKVCDQVSDAILDAYLAQDPASRVAC
                     ECLATTDRMTIAGEITSKGKVDADKVARNVIKKIGYVDSRIGFDYKTCKIDVALHTQS
                     PDIAMGVDTGGAGDQGMMFGYATDETPEFMPLPIMLAQKLVRYLTAVRKSGKIKYLRP
                     DSKSQVTVEYKNEKPVRVEAVVLSSQHDGNVTLSTIQKDLTEKVIKKVIPANLMDKNT
                     KIFINPTGRFEVGGPHGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYFA
                     RYVAKNIVAAGLAGSCEIQVSYAIGVAKPVSIRVDTFGTNRISEEAIVEAVTKVFDFT
                     PRGIISALDLLRPIYNKTAAYGHFGRSEAGFTWEKTDKVDALRKVLKLKDGCACCKVT
                     TAV"
     gene            complement(2345..3082)
                     /locus_tag="PLY30_01590"
     CDS             complement(2345..3082)
                     /locus_tag="PLY30_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPW64825.1"
                     /translation="MKVILLCAGYATRLYPLTENQPKPLLPVGGRPILEWILDRVGKV
                     SGIEAVYIVSNAKFAGHFAAWAASAKYPWPVEVVDDGTSSNETRLGAIGDLAYTIRAK
                     ALRPDDLMIIAGDNFFDFDLTALTDYGGKVRPHGLIAVYDVGDRELAKRYGLVRTSED
                     GKVLEFQEKPPEPKTTLASCGIYWLPKEIWGLLDTYIKGGHNTDQPGHYMRWLAEISG
                     LYAFPLKGKWLDIGDIASYQKANAIVG"
BASE COUNT          620 a          993 c          894 g          736 t
ORIGIN      
        1 atcgcctcat ccttcatttt ggacatgtgc acggcggtga tgacgtcgat attaccggtc
       61 gtcgtcacgt agatatcgcc gatatcgagc acatcctcga gacgcgcgac ctggtagccg
      121 gccatggacg cctgaagggc gcagatcggg tcgatctcgg tcacgatcac gcgagccttc
      181 aattccgcga gggattgagc gctgcctttg cccacgtccc catagccgag aacaacggcg
      241 accttgcccg cgatcatcac atcggtcgcg cgtttgatgc cgtccacgag cgattcgcgg
      301 cagccgtaca ggttgtcaaa tttcgatttc gtgacggaat cgttcacgtt gatcgcggga
      361 acgaggagcc ttccctcttc ctgcatcttg tagagccggt gaacgccggt cgtggtctcc
      421 tccgaaacgc cttcccattc cgcgacaacg tcgcgccatt tcttcggggt ctcctcgagg
      481 cattgtttca ggaccctgaa aagctcgatc tcatcctgcg tctcgacctt cttgtcgaga
      541 agtctggggt tcttttccgc gtcgtatccg agatggacca taagcgtcgc gtccccgccg
      601 tcatcgacga tcaggttcgg gcccttgccg cccgggaagg tcagggcctg caacgtacac
      661 caccagtatt cttcgagggt ctcccctttc cacgcaaaca ccggaatccc gcgcttcgcg
      721 atcgcggccg ccgcgtggtc ctgcgtcgaa aagatattgc acgagcacca ccggacatcc
      781 gcgccaagct cgacgagtgt ctcgatcagc accgcggtct ggatcgtcat gtggaggctc
      841 cccatgatcc gtttgccctt cagcggtttt ttcggtccgt attttctgcg ggtggccatg
      901 aggccgggca tttccttctc ggccatttcg atctctttac ggccccaatc cgccagggaa
      961 aggtccgcga ccttgaagtc ctggatcttg ttctgggcag gctgtttctt tgactcggta
     1021 gacatgtttt cctttctttt ttcgacggga gatcacggcc ccgggacgcg agggttcacg
     1081 ccccgggaac atgcgatggt taaacggccg tcgtcacttt gcagcaggcg cagccgtcct
     1141 taagcttcag gactttgcgg agcgcgtcaa ccttgtcggt cttctcccac gtaaaaccgg
     1201 cttccgaacg gccgaaatgc ccgtacgcgg cggtcttgtt gtagatcgga cggagaaggt
     1261 ccagcgcact gatgatcccc cgcggggtga aatcaaaaac cttcgtcaca gcttccacga
     1321 tggcttcctc gctgatcctg ttggtgccga aggtatccac acggatcgaa acgggcttcg
     1381 ccacgccgat cgcataagaa acctgtatct cgcagctgcc cgcgagaccg gcggccacga
     1441 tattcttcgc cacataacgg gcaaaatacg ccgcggagcg gtccaccttc gacggatcct
     1501 tgccgctgaa cgcgccgccg ccgtgacggc ccatgccgcc gtaggtgtcg acgatgatct
     1561 tgcggccggt aagcccggtg tccccgtggg gaccgccgac ctcgaaacgc ccggtcgggt
     1621 taatgaagat tttcgtgttt ttgtccatca ggttcgccgg gatgactttc ttgatcactt
     1681 tttcggtcag atccttctgg atggttgaaa gtgtcacatt gccgtcatgc tgggaggaga
     1741 gcaccaccgc ctcgacgcgc accggctttt cgttcttgta ttcgaccgtg acctggctct
     1801 tcgaatccgg acggaggtac ttgatcttgc cggatttgcg gaccgccgtg agatagcgga
     1861 caagtttttg ggcgagcatg atcgggagcg gcatgaattc cggcgtttca tcggtcgcat
     1921 agccgaacat catgccctgg tctccggcac cgccggtgtc cacgcccatc gcgatatcgg
     1981 gcgactgggt gtgaagcgcg acgtcgatct tgcaggtctt gtagtcaaaa ccgatcctgg
     2041 aatccacata accgatcttt ttgatcacgt tacgggcaac cttgtcggca tcgaccttgc
     2101 ctttactcgt gatctcgccg gcgatcgtca tgcggtccgt cgtcgcgagg cattcgcagg
     2161 cgacgcggga ggccggatcc tgggccagat aggcgtcaag gatcgcgtcc gaaacctggt
     2221 cacagacctt gtccggatgt ccctcagaaa cggattcgga ggtgaaaaaa taattccctt
     2281 ttttcatttt gttgctcctt tggtttattt tcactgactc cacagtccta cgactagcgg
     2341 aaaatcatcc gacgatagcg ttcgctttct ggtaggaggc gatgtcgccg atatccagcc
     2401 atttcccttt gagagggaat gcgtaaagcc ccgagatctc ggcaagccac cgcatataat
     2461 gtccaggttg gtccgtatta tggcccccct tgatataggt gtcaaggagg ccccagatct
     2521 ccttcggcag ccagtaaatg ccgcacgaag caagcgttgt cttcggctcc ggaggctttt
     2581 cctggaactc caggaccttc ccatcctccg aggtccgcac gaggccgtag cgctttgcga
     2641 gctctctatc ccctacgtcg tagaccgcga tcaagccgtg cggccggacc ttcccgccgt
     2701 agtccgtcaa cgccgtcaga tcaaaatcga agaaattatc ccccgcaata atcatcaggt
     2761 catccggccg gagcgctttc gcgcgaatcg tgtaggccag gtccccaatg gccccgagcc
     2821 tcgtctcatt cgaggatgtc ccgtcatcaa caacctcgac cggccaggga tatttcgcgg
     2881 aagcggccca cgccgcgaaa tgccccgcaa acttcgcgtt gctcacgata tagacggcct
     2941 cgatccccga aaccttcccg acccggtcca gaatccattc gagaatgggt ctgccgccca
     3001 cgggaagaag gggttttggc tggttctcgg tcaagggata aaggcgggtc gcgtagcccg
     3061 cgcaaagaag gatgactttc atttgatccc gggaacggct ttcttgatgc ggtcgatcac
     3121 gcagatggac atcggatcga cgcgattgag tttcgcaagc tcacagcccg cccagtcgcc
     3181 aagcatcacc atcgagaaga tacgcgagag gcatttttcc cctttcgaac ggaactccat
     3241 gac
//
LOCUS       DAOOBX010000071         3222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_33223_length_3222_cov_3.477423, whole genome shotgun sequence.
ACCESSION   DAOOBX010000071 DAOOBX010000000
VERSION     DAOOBX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33223_length_3222_cov_3.477423"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>722)
                     /locus_tag="PLY30_01595"
     CDS             complement(<1..>722)
                     /locus_tag="PLY30_01595"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPW64826.1"
                     /translation="CGSCAGMFTANSMNCLAEAIGLALPGNGTIPAAAWTDTKALSWV
                     INPERVALVEKAADVLEEFLKKKIRPRDIVTKRSIDNAFILDLAMGGSTNTVLHTLAL
                     ACEAGIDYPLERINLLARKTPNLCKVSPSRPDVHMEDVHRVGGIAAILKAVAEDGRAP
                     LSLDLKTCTGTLRDMVEKAPDADGDVIRTGEKVFSREGGLAVLYGNIAPQGAVVKTAG
                     VEKDMMKFTGRAKCYDSQDLAL"
     assembly_gap    723..732
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<733..1370)
                     /locus_tag="PLY30_01600"
     CDS             complement(<733..1370)
                     /locus_tag="PLY30_01600"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPW64827.1"
                     /translation="MPKAINEGFEAIRKKGSRVVAEGNPMLPAAGLMVATGTLSSVHD
                     RKRPFVTVINSYTTQIPGHVHLNVLGEKVVNALKAKGCNVWYANIGAAICDGIAMGHF
                     GMKYSLPSRELIADQIESIIGAHPCDAWIGIGNCDKIIPAMLNSMVRLNIPSVYVSGG
                     PMLAGRNKSDLITVFEGVGKEAAGKISSDQLAEMANSACRTCRARSALSLAS"
     gene            complement(1388..2347)
                     /gene="nadA"
                     /locus_tag="PLY30_01605"
     CDS             complement(1388..2347)
                     /gene="nadA"
                     /locus_tag="PLY30_01605"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPW64828.1"
                     /translation="MSQSQNTQSPKEILQEIETLKRERNAVIIAHNYQPSEIQDLADY
                     TGDSLDLSRIAAQTSASVIVFCGVKFMGETAAILSPGKTVLLPVPEAGCTLADHASAE
                     EIREMKKKHPGALVVTYVNSSAAVKAESDICCTSANAVDIVKAIDPSRRIIFAPDYNL
                     GHYAAKKTKRDMILWNGCCPTHALLSTLAVEEARKKWPKAIFMMHPECRPQALELADY
                     IGSTSAMIRYAKQQPAGTEFIVGTEIGLLHRLRKDNPEKTFHVASEFLVCPMMKMISL
                     RDVRDSLKKMQHVVSVPEDIRMKAKAAVEAMLLYTGNPAKTAD"
     gene            complement(2437..>3222)
                     /locus_tag="PLY30_01610"
     CDS             complement(2437..>3222)
                     /locus_tag="PLY30_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW64829.1"
                     /translation="PGYQYIYGPVASWRYGRSLGIDPVSAAKKTCSFDCVYCQVGKTV
                     LLTDQRSVFVPTEALIGELQRVPPVEIDQITFAGNGEPTLAANLGEMIRGIRNIRSER
                     IAVITNASLIWRPDVREDLALADVVIAKLDAATETMFSDVNHPAAKLTIEEICGGLRV
                     FKKKFKGRMVLQAMFVSPNRDAAAGIAKRALSIGPDEIEVNTPLRVSPVKPLGPEEMA
                     GITKIFREICAGIPVKTVYEEERKKSEPFCRESTERRRGKESA"
BASE COUNT          610 a         1000 c          893 g          709 t
ORIGIN      
        1 gagcgcgaga tcctgggaat cgtaacattt cgcgcgtccc gtgaatttca tcatatcctt
       61 ttcaacgccg gccgttttca cgacggcgcc ctggggagcg atattcccgt aaaggaccgc
      121 aagcccacct tcccgggaaa acaccttctc tccggtccgg ataacatccc cgtcggcgtc
      181 cggggccttt tcaaccatat cccgcagcgt gcccgtgcag gtttttagat cgagggaaag
      241 cggggcgcgt ccgtcctccg cgaccgcctt gaggatcgcc gcgatgcccc cgacgcgatg
      301 aacatcctcc atatggacat cggggcgcga cggcgacacc ttgcaaaggt tcggcgtttt
      361 cctggccagc aggttgatac gttcaagcgg ataatcgatt ccggcctcgc aggcgagcgc
      421 aagcgtgtgc aggaccgtat tcgtcgaacc gcccatcgcg aggtccagga tgaacgcgtt
      481 atcgatcgat cgtttcgtca cgatatcgcg cggacggatc ttcttcttga gaaactcctc
      541 cagaacatcc gcggcctttt cgacaagcgc gacgcgttcg ggattgatga cccacgaaag
      601 cgccttcgta tccgtccatg ccgccgcggg tatcgtcccg ttgcccggga gtgcgaggcc
      661 gatggcctcc gcgagacagt tcatggaatt cgccgtgaac atgcccgcgc agcttccgca
      721 ggnnnnnnnn nncaactcgc cagcgaaagc gctgacctgg cccgacaggt acggcacgcc
      781 gaattcgcca tttcggccag ctggtcggac gatatcttgc ccgccgcctc cttgcccacg
      841 ccttcgaaaa ccgtgatgag atcggacttg ttgcggcccg cgagcatcgg cccgccgctc
      901 acataaaccg aggggatatt gagccggacc atcgagttca gcatcgcagg aataatcttg
      961 tcacaattgc cgatcccgat ccacgcgtcg cagggatggg ccccgatgat cgattcgatc
     1021 tgatccgcga tcagttcccg cgacggaagc gaatacttca tgccgaaatg ccccatcgcg
     1081 atcccgtcac agatggccgc gccgatattg gcgtaccaaa cgttgcaccc ctttgccttg
     1141 agggcattga cgaccttttc gccaaggaca ttcagatgga catgcccggg gatctgtgtc
     1201 gtgtaggaat tgatgaccgt gacgaacgga cgcttgcgat cgtgaacact cgacagcgtt
     1261 cccgtcgcaa ccatcagacc ggcggccggc agcatgggat taccttccgc cacgacgcgg
     1321 ctcccttttt tccggatcgc ttcaaaccct tcattgatcg ctttgggcat aaacaattcc
     1381 tttcgaatta atcagccgtt ttggccgggt ttcccgtata aagcagcatg gcctccacag
     1441 cggctttcgc cttcatgcgg atatcttccg ggacactgac gacatgctgc atttttttga
     1501 gcgaatcccg gacatctctc agggaaatca ttttcatcat ggggcagacc agaaattcgc
     1561 tcgcaacgtg gaaggtcttt tccgggttgt ccttccggag acgatgcaaa agtccgattt
     1621 cggtcccgac gataaattcc gtcccggcgg gctgttgttt cgcgtagcgg atcatcgccg
     1681 acgtgctgcc gatataatcc gccagctcga gcgcctgcgg acggcattcg gggtgcatca
     1741 tgaagatcgc cttcggccat ttcttcctgg cctcttcgac ggccagcgtt gaaaggagcg
     1801 cgtgcgtcgg acagcagccg ttccaaagga tcatgtcccg tttcgttttt ttcgcggcgt
     1861 agtgaccgag gttatagtcg ggtgcgaaaa tgatccgcct cgagggatcg atcgccttga
     1921 cgatatccac ggcgttcgcg gacgtgcaac agatatcact ctcggccttc acggccgcgg
     1981 agctattcac gtaagtcacg acgagagcgc ccgggtgttt ctttttcatt tcccggatct
     2041 cttcggccga cgcgtgatcg gccagcgtgc agccggcctc cggaacaggc aggagaacgg
     2101 tctttccggg cgaaaggatc gcggccgtct cccccatgaa cttcacgccg cagaagacga
     2161 tgacactcgc ggaggtctgc gcggcgatgc ggctgagatc gagggagtcc ccggtgtaat
     2221 cggccaggtc ctggatctcg gacggctgat aattatgcgc gatgatgacg gcattccgct
     2281 cgcgcttcag ggtctcgatc tcctgaagaa tctctttcgg ggattgtgta ttctgggatt
     2341 ggctcattaa tggctccgaa gtcgggatat cttacgtttt ttcccttcgt cttgcaatga
     2401 actctcttgc ggccgcgctt tcacgacacg gcgatctcag gcgctctcct tgccccgccg
     2461 gcgctcggtc gattccctgc agaacggctc gcttttcttc cgctcctctt cataaacggt
     2521 tttgacgggg attcccgcgc agatctcccg gaagatcttc gtgatccccg ccatctcttc
     2581 cggcccgagc ggcttcaccg ggctgacgcg aagaggcgtg ttaacctcga tctcgtcggg
     2641 accgatcgaa agcgctcttt tcgcgatccc ggcggccgcg tcccggttcg ggctgacgaa
     2701 catggcctgg agcaccatcc gtcccttgaa tttcttttta aaaacgcgca atccgccgca
     2761 gatctcctcg atcgtcagct tcgccgcagg atgattcacg tcgctgaaca tggtttcggt
     2821 cgcggcatca agtttcgcga tcacgacatc cgcaagcgcg aggtcctccc gcacatcggg
     2881 ccgccagatc agggacgcgt tcgtaatcac ggcgattctt tcggagcgga tattccggat
     2941 tccgcggatc atttccccca gattggccgc aagcgtcggt tccccgttcc ccgcgaacgt
     3001 gatctggtcg atctcgaccg gcggcactct ctggagttct ccgatcaggg cctcagtcgg
     3061 aacgaaaaca ctccgctgat ccgtcaggag caccgttttc cccacctggc aatagacaca
     3121 gtcgaaagaa caggtcttct tcgcggccga aaccggatcg atcccgaggg aacgtccgta
     3181 ccgccaggaa gcgaccggtc cgtagatgta ttgataaccc gg
//
LOCUS       DAOOBX010000072         3205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_33428_length_3205_cov_3.333333, whole genome shotgun sequence.
ACCESSION   DAOOBX010000072 DAOOBX010000000
VERSION     DAOOBX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33428_length_3205_cov_3.333333"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLY30_01615"
     CDS             complement(<1..521)
                     /locus_tag="PLY30_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64830.1"
                     /translation="MAHTYRDHPDPRKRKAPSQKKLKKPEARTIWVGDTATIERHFLR
                     LSKLLRSEERSERDRFQMDAARKPEERERGGRALYRLELLEVHYNPSGHKLLTFALKT
                     KRPLPRFSLDAGDIVKLSGFQTPAAECPTGAVYERDRMRITVAFSGHLPAWIDREDSF
                     QLSAAENLYTYER"
     gene            complement(581..997)
                     /locus_tag="PLY30_01620"
     CDS             complement(581..997)
                     /locus_tag="PLY30_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPW64831.1"
                     /translation="MKSHTEYLSINTEERYEIVHLTPRIEEAVRKSGVSEGLCLVNSM
                     HITSSVFINDNEHGLHQDFLAWVEKLAPYGEDKYKHNLTGEDNGDAHLKRTLMGREVV
                     VAITKGRLDLGPWEQIFYGEFDGRRKKRVLIKIIGE"
     gene            complement(1096..1452)
                     /locus_tag="PLY30_01625"
     CDS             complement(1096..1452)
                     /locus_tag="PLY30_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HPW64832.1"
                     /translation="MKKTTTVIIVLALTILIPSAWAASMQADKAVSKLARGTTNMASC
                     WGEYITQYPAAVEKSPDHLTAAIYGVFRGTAYTIRRAMVGLYDVVTSPFPGRTNYGPV
                     IQPETIFNKTAETLSH"
     gene            complement(1537..2085)
                     /gene="amrA"
                     /locus_tag="PLY30_01630"
     CDS             complement(1537..2085)
                     /gene="amrA"
                     /locus_tag="PLY30_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPW64833.1"
                     /translation="MAKKGMPAQSEQQKILNYIRELLKARLTRGPEPELGLSREFLDQ
                     KRGIFVTLKRNGALRGCIGQILGYKPLRESIREMAIAAAFEDPRFPPLRREELKDLHI
                     HVSLLTEPKPVPSYRDIRVGTDGLIVSLGRRKGVYLPEVATETGWDARTFFMSCAIEK
                     AGIDETELDQVTIEVFQTEGFE"
     gene            complement(2124..2963)
                     /gene="amrB"
                     /locus_tag="PLY30_01635"
     CDS             complement(2124..2963)
                     /gene="amrB"
                     /locus_tag="PLY30_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPW64834.1"
                     /translation="MKPRSKVRSAVVSGQFYSDMPKVLRGDIEAFLDRAVSAPDPSVR
                     GIIVPHAGYMYSGPVAAEAYRAIRGASVDTVVIVSFLHRVFIQGILVDDADFYETPLG
                     RIPVERELCAAIRAEAPCFAEAVEGDLNEHSLEVQLPFLQFAIGDFSVVPVYMGEQSV
                     ANARALSDALAKHLAGRRALVVFSTDLSHFHPYHEAVRMDQKFIRMTGDRDFMKLCRS
                     SDSGEIEACGLGPVITSLMLAETLGWDGPRLLKYANSGDVTGDKSSVVGYAAMVYKTG
                     GKR"
     gene            complement(3016..>3205)
                     /locus_tag="PLY30_01640"
     CDS             complement(3016..>3205)
                     /locus_tag="PLY30_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HPW64835.1"
                     /translation="AISLSARILSVADVYDALITARPYRKALPKEEAVRMIEAEAGSH
                     FDPKIVRIFLELVRNGEI"
BASE COUNT          659 a          943 c          911 g          692 t
ORIGIN      
        1 atccgctcat aggtgtaaag attctcggcg gcggacaact gaaaactgtc ctcccggtcg
       61 atccacgcgg gaagatgtcc gctgaaggcc accgtgatcc gcatccggtc ccgttcatac
      121 acggctcccg tcgggcattc cgccgcggga gtctgaaacc ccgaaagctt cacgatatct
      181 cccgcgtcca ggctgaagcg cggcagcggg cgcttcgttt taagggcgaa tgtcaggagt
      241 ttgtgccccg agggattgta atggacctcg agcagttcga gccggtaaag ggcccggcct
      301 ccgcgttcac gctcttcagg tttccgggcc gcgtccatct ggaaccggtc ccgctccgag
      361 cgttcctcgg atcgaagcag tttcgagagg cggaggaaat gccgctctat cgtcgcggta
      421 tccccaaccc agatcgtgcg cgcttcgggt tttttgagtt tcttttggga aggagctttt
      481 ctttttctgg gatcgggatg atcccggtat gtatgcgcca taaaatctct taaaatcagg
      541 atcacattcc gatcttgatt ggggtgtggc cccgatttgt ttattcgccg ataattttga
      601 tcagcacgcg ctttttgcgc ctgccgtcga actcgccgta aaaaatctgt tcccacggcc
      661 cgagatcaag ccggcccttc gtgatcgcga ccacgacttc gcgccccatg agcgtgcgtt
      721 tcagatgggc atcgccgtta tcctccccgg tcaggttatg cttgtacttg tcttcgccgt
      781 aaggggccag cttctcgacc cacgcgagaa aatcctggtg caatccgtgc tcgttatcgt
      841 tgatgaaaac gctcgatgtg atgtgcatgg agttcacgag gcacaacccc tcgctcacgc
      901 cgcttttccg gaccgcttct tcgatccggg gcgtgagatg cacgatctca taacgctctt
      961 ccgtatttat cgaaagatat tcggtatggg acttcacggg caaacgctcc ttgagacatg
     1021 gattctatca cagaacgccg gaagaaagag ccggcgtctt ttctgaaacg gaatccccag
     1081 aggataaaat ccggatcagt gggagagagt ttcagcggtc ttgttgaaaa tggtctcggg
     1141 ctggattacg gggccgtaat tggtgcggcc ggggaacgga gatgtcacaa catcatagag
     1201 cccgaccatc gcgcgacgga tcgtataggc cgtcccgcgg aacacgccgt agatcgcggc
     1261 cgtcagatga tccgggcttt tctccactgc cgccgggtac tgggtgatgt attcgcccca
     1321 gcagctcgcc atattcgtgg taccccgcgc cagttttgaa acggctttat ccgcctgcat
     1381 ggaggccgcc caggccgacg gaatcaggat ggtcaatgca aggacaatga tgacggttgt
     1441 tgtttttttc atgctcgcac tccttatcga taagaggatc gggggtttat cccgccctta
     1501 tagcatcgtc cgtcccgtga atttattgaa tggttattat tcaaaccctt cggtctgaaa
     1561 gacctcgatc gtcacctgat cgagctccgt ttcgtcgatc ccggctttct cgatcgcaca
     1621 gctcataaaa aaagtcctcg cgtcccagcc cgtttcggtc gcgacctccg gaagatagac
     1681 accctttctc cgaccgaggc tcacgatgag accgtccgtc ccgacgcgaa tatcgcggta
     1741 ggacggaacg ggtttcggct ccgttaaaag ggaaacatgg atatgaaggt ccttcagctc
     1801 ctcccgccgc agaggcggga atcgcgggtc ctcaaatgcc gccgcgatcg ccatttcacg
     1861 gatactttcc cggagaggtt tatagccgag aatctgtccg atgcagccgc ggagcgcccc
     1921 gtttcttttc agcgtcacaa agatcccccg tttctgatcg aggaattccc gggagagacc
     1981 gagctccggc tccggacccc gtgtgaggcg cgctttgaga agttcgcgaa tgtagttaag
     2041 gattttttgt tgttccgatt gagccggcat gcctttttta gccatcacca ttaggcgaca
     2101 ggcggcaggt tttgacttag cccctaccgc ttgccgccgg ttttataaac catcgccgcg
     2161 tagccgacca ccgaactttt gtcaccggtc acatcaccgg aattcgcgta tttcagaagc
     2221 cgggggccat cccacccgag cgtctcggcg agcatcagcg aggtaatgac gggacccagc
     2281 ccgcaggctt cgatctcccc cgaatcgctt gaacggcaga gcttcatgaa atcccggtcc
     2341 cccgtcatgc ggatgaactt ctggtccatg cggaccgcct cgtgataggg atgaaaatgc
     2401 gaaagatcgg tgctgaacac gacaagcgcc cgccgtcccg cgagatgttt cgcgagcgcg
     2461 tccgaaaggg cccgggcgtt cgcgaccgac tgttcgccca tgtagaccgg aacaaccgaa
     2521 aaatccccga tcgcgaattg aaggaacggc agctgaacct ccagggaatg ttcgttgaga
     2581 tcgccctcga ccgcttccgc gaaacagggg gcttccgcgc ggatcgccgc gcaaagttcc
     2641 cgctccacgg ggatccgccc aagcggcgtc tcgtagaaat ccgcatcatc aacaaggatc
     2701 ccctggatga aaacgcggtg caggaaagag acgatcacaa ccgtgtcgac gctggcgccg
     2761 cggatcgccc gataagcttc cgccgcgacc ggtcctgaat acatgtagcc ggcatgaggc
     2821 acgatgatcc cgcgaacgga gggatcgggc gctgagaccg cccggtcgag aaaggcttcg
     2881 atgtcgccgc gcaggacctt gggcatatcg gaatagaact gtccgctgac gacggcggac
     2941 cggactttcg aacggggttt catgatatcc tccgaatgac cggcgccggc aaaagacggg
     3001 tcgctggccg gttggtcaaa tctctccgtt ccggaccagt tcgaggaaga tccggacgat
     3061 cttggggtca aaatgggagc cggcctccgc ttcgatcatt cgcacggcct cctctttcgg
     3121 aagggcctta cgatacggcc gcgccgtgat gagagcgtca tacacatccg ccacggaaag
     3181 aatgcgggcg ctgagggaaa tcgcc
//
LOCUS       DAOOBX010000073         3191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_33598_length_3191_cov_4.916773, whole genome shotgun sequence.
ACCESSION   DAOOBX010000073 DAOOBX010000000
VERSION     DAOOBX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33598_length_3191_cov_4.916773"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..893)
                     /locus_tag="PLY30_01645"
     CDS             complement(117..893)
                     /locus_tag="PLY30_01645"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HPW64836.1"
                     /translation="MEKAIGLVGGMGPYAGLDLLKKVYDNVDVHSDQGYPDVMLISAS
                     RLIPDRSAYIKNPETENPAVGISYCVRKLADMGATHIGVSCNTAHAPIIMNKVRAFIE
                     REGLSVSLLSIVAETRDHVVRNIKPGRIALFATEGTYRSGIYRDSFCDDPQYEIVEPE
                     NDEKQLIWDAIYSKEFGIKSMSSPVQTQAVENFRVVAERMMERDGIDTFIMGCTEIPL
                     GMNRLKLPATLIDPAVILARALVGAVVPEKLKRAQYPAFA"
     gene            complement(910..3021)
                     /locus_tag="PLY30_01650"
     CDS             complement(910..3021)
                     /locus_tag="PLY30_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPW64837.1"
                     /translation="MMKIKIRDALIIVNTLLIVATAAVIITINSTGSKASVYSISNNL
                     VMEILNSVVNKIQVYFHPAEKAVRDIGFHYWTKDSGDFAKNKEIAVDYFIETLRTNPE
                     FVMVYFADTEGNLVMGRRMPDGTLSKRYVSRTADSVVTTFAHENKEYIPAYPNAVDSL
                     EKGYDPRSRGWYQKAVQMKTLVWTDVYIFATDKMPGFSCALPVYNDDGTLKGVVCIDI
                     GIRDLSLFLGKINTTDNSKLFIVDGNDSVLAKPLQIEESLNTLFNIQDKGGDTEYSLK
                     KVDDPSDPVIAAAYASFGEQTDTSSIFSFMLSGKKYFSKIATIELEKGMKLKIGMVVP
                     DEDIMGIVYRNNKFVLLISLVVIIIAILISFIVSAFISSPMDILSKEMNGIKTLEISD
                     DSEISTNIVEIAGMVDSFQGMKKGLTNFKKYVPSDLVNILMQNNQDANIGGVRKEVTM
                     YFSDIENFTGISEKLMPEELLKRLYEYFSLMSNAIHDNKGTLDKYIGDSIMAFWGAPL
                     DLDDHAACACRSALTCRDLGNNLSNAWQREGKDRFRTRMGIHTGEVVVGNMGSDERIN
                     YTVLGDNVNLASRLEGINKYYGTEITVSDSTHALVKDQFEFRMLDKIAVKGKTQPVTI
                     YELLSEKDKYDKNKLKVYGYYETGLSYYFGGNWDKTIQYMDVVLKYLKDPPAATIRSR
                     AVEYRVNPPENWNGVHSFKDK"
BASE COUNT          614 a          880 c          939 g          758 t
ORIGIN      
        1 aaccgaattt tgacccgaaa ccgcaaaaaa cccgaaatga cagttcctat cagtcccgtc
       61 gaggcggcgt gccgcgcgtt cgagaatatc gtcggcgagc tggagatcgc gctcgcctag
      121 gcgaacgcgg gatattgggc gcgcttgagc ttttcgggaa cgacggcgcc gacaagggcg
      181 cgcgcgagaa tgaccgcggg atcgatcagc gtcgcgggaa gcttcaggcg gttcatgcca
      241 agcgggattt ccgtacagcc catgatgaat gtgtcgatcc cgtcgcgttc catcatccgc
      301 tcggcgacga cgcgaaagtt ctcgacggcc tgcgtctgga cggggctgct catcgacttg
      361 atgccgaatt ccttgctgta gatcgcgtcc caaatcaact gcttttcgtc gttctcgggc
      421 tcgacgatct cgtactgcgg atcgtcgcaa aacgaatcac ggtaaatgcc tgaccggtat
      481 gttccctcgg tcgcgaaaag ggcgatgcga cccggcttga tattcctgac gacatgatcg
      541 cgcgtctccg cgacgatgct gagcagactc accgaaagac cctcgcgctc gataaacgcg
      601 cggaccttgt tcatgatgat cggcgcgtgc gcggtattgc acgagacgcc gatatgcgtc
      661 gcgcccatgt cggcgagctt tcgcacgcag tacgaaatac cgacggcggg attctcggtc
      721 tccggatttt tgatgtacgc cgagcggtcg ggaatgaggc gggacgcgga gatgagcatg
      781 acgtccgggt atccctgatc ggagtggacg tcgacgttgt catagacttt tttcaggaga
      841 tcgagcccgg cgtacgggcc cattcccccc acaagaccga tagccttttc cattttatgt
      901 atcctttttt tatttatcct tgaacgagtg caccccgttc cagttttccg ggggattgac
      961 gcggtactcg acggccctgc tccgtatcgt cgcggccggc gggtccttca ggtatttcag
     1021 gacgacgtcc atgtactgga tggtcttgtc ccagtttccg ccgaagtagt acgacaggcc
     1081 ggtttcgtag tagccataga ccttcagctt gttcttgtcg tacttgtctt tttcggacag
     1141 caactcgtag atcgtgacgg gttgcgtctt tcccttgacc gctatcttgt cgagcatgcg
     1201 gaattcgaac tggtccttca cgagcgcgtg agtcgagtcg ctgacggtga tctcggtgcc
     1261 gtagtacttg ttgatgccct cgaggcggct cgcgaggttc acgttgtcgc cgagcacggt
     1321 gtagttgatg cgctcgtcag agcccatgtt tccgacgacg acctcgccgg tgtgtattcc
     1381 catgcgcgtc cggaagcggt ccttgccctc gcgctgccag gcgttcgaca ggttgttgcc
     1441 gaggtcgcgg cacgtgagcg cggatcggca cgcgcacgcg gcgtgatcgt cgaggtcgag
     1501 gggcgcgccc cagaatgcca tgatcgagtc gccgatgtac ttgtcgagcg ttcccttgtt
     1561 atcgtggatc gcgttcgaca tgagcgagaa gtattcgtac agccgcttca gcaattcttc
     1621 cggcatcagc ttttcggata tcccggtgaa gttctcgatg tcggaaaaat acatcgtgac
     1681 ttccttcctg acgccgccga tattcgcgtc ctgattgttc tgcatgagga tgttgacgag
     1741 gtccgacggc acgtacttct tgaaattggt caggcccttc ttcataccct ggaacgagtc
     1801 gaccattccc gcgatctcga cgatgttcgt cgaaatctcc gaatcgtcgg atatctcgag
     1861 cgtctttatc ccgttcattt ccttggaaag gatatccatc gggctcgata tgaacgcgga
     1921 aacgatgaac gatatcagga tcgcgatgat gatgaccacg agcgagatga ggaggacgaa
     1981 cttgttgttg cggtacacga tccccatgat gtcttcgtcg gggacgacca ttccgatctt
     2041 gagcttcatg cctttctcga gctcgatcgt cgcgatcttc gagaaatatt tctttccgga
     2101 gagcatgaac gagaaaatcg acgacgtgtc ggtctgctcg ccgaaactcg cgtatgcggc
     2161 cgcgatgacg ggatccgacg gatcgtcgac ctttttcagc gaatactccg tatcgccgcc
     2221 cttgtcctgt atattgaaga gcgtattgag cgattcctcg atctgcagcg gtttcgcgag
     2281 cacgctgtcg ttaccgtcga cgatgaaaag tttcgaatta tcggtcgtgt tgatcttgcc
     2341 gaggaagagc gaaaggtccc gtatgccgat gtcgatgcag acgacgccct tgagcgtgcc
     2401 gtcgtcgtta tagacgggaa gcgcgcacga aaagcccggc atcttgtcgg tcgcgaaaat
     2461 gtacacgtcg gtccagacga gcgtcttcat ctgcacggcc ttctggtacc agccgcgaga
     2521 gcgcgggtca tagcccttct cgagcgagtc gacggcgttc gggtacgcgg gaatgtattc
     2581 cttgttctcg tgcgcgaacg tcgtgacgac ggagtcggcg gtcctcgaga catagcgctt
     2641 cgaaagcgtc ccgtccggca tcctgcggcc catgacgagg tttccctcgg tgtccgcgaa
     2701 gtacaccatg acgaactcgg gattcgtccg gagcgtctcg atgaaatagt cgacggcgat
     2761 ttccttgttc ttcgcgaagt ccccgctgtc cttggtccag taatggaagc cgatgtccct
     2821 gacagccttc tccgccggat ggaaatatac ctgtatcttg ttgacgacgg agttgagaat
     2881 ttccatcacg aggttgttcg atatcgagta gacggacgcc tttgatcccg tcgagtttat
     2941 cgtgatgatc accgccgcgg tggcgacgat gagcagcgta ttgacgatga tgagcgcgtc
     3001 ccgaatcttg attttcatca tttggccccg aaggttgacc gttcgttatc ggcgataagc
     3061 gtcgtcgtcg cgatgttcgc gtacacgatc aggaacgtgc gcatcgggtc gatgagcgag
     3121 tcgatcgcca tgaacacgat caggatcgac tcgacgggaa gcccgagcgg gtcgaggctg
     3181 aggctgatca t
//
LOCUS       DAOOBX010000074         3190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_33610_length_3190_cov_4.205423, whole genome shotgun sequence.
ACCESSION   DAOOBX010000074 DAOOBX010000000
VERSION     DAOOBX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33610_length_3190_cov_4.205423"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLY30_01655"
     CDS             <1..531
                     /locus_tag="PLY30_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HPW64838.1"
                     /translation="PGTAPVKRGKQIFLVHRLDKEVSGLLMFAKTAGMKEILRANWPD
                     VTKRYYAVVEGVPEKPEGTLKSYLRENKFLKVYSTPATEGAKLSVTHYRVMRSSPRHS
                     LLEVELGTGRKHQIRVQLSDMGHPIAGDSQYGARTDPAKRIALHSHYLAFVHPVTGKE
                     TEIDLPVPAAFEKIVT"
     gene            645..1109
                     /locus_tag="PLY30_01660"
     CDS             645..1109
                     /locus_tag="PLY30_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64839.1"
                     /translation="MNKGNEQAGGNQEEEFGEREEDREDEMEEVDITEVLEKINERLE
                     ILEKKLNFLIERSENAGNRAEGKQDSRGFREHRKPFGRPFRGGGGGFKPRHGGGGFHT
                     GRRDDQDGGFGRHHESPERSFGRSHGAAGSFRKKRFGRGGGHRKGSRGSREF"
     gene            complement(1224..2636)
                     /locus_tag="PLY30_01665"
     CDS             complement(1224..2636)
                     /locus_tag="PLY30_01665"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPW64840.1"
                     /translation="MGPVESLRHFIPELVLLGGAFTAFFLDFLLKNKRLVGYFALLTI
                     VAAACLLKAPPKESLDLFYGFFHLDGFANLFRVLALIAVAATILIAFGFSPLRDRYEG
                     EFYGLFLFMAFALITVAAANNLLMIFLSVEFVSILSYLLAGFLKNDLKAKESAVKYLL
                     FGSVCSAVMLYGMSLLFGAAGSIDLPAIARVVDSPAMKPLGMIGLLCFFVGLGFKISM
                     APFHMWAPDVYEGAPTPVTAFLTVAPKALGFAVLMRVLASVFGESYRDWRQVLMWLSI
                     LTMTLGNLTAISQTNIKRFLAYSSIAQAGYILMGLAVFPSPLGKDGVLIYLFAYLFTN
                     LGAFAVVSSVGDRTGSDEIASYSGLSERAPVSAALMTVFLLSLTGIPPLAGFLAKYYV
                     FAAAVEARFITLVVVAAVNSAVAAYYYFRVIRTMYLVPADQPALPKPPVSAAAVTWLM
                     AAGVLGIGLFPGFFIKLILG"
     gene            complement(2637..>3190)
                     /locus_tag="PLY30_01670"
     CDS             complement(2637..>3190)
                     /locus_tag="PLY30_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010477435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPW64841.1"
                     /translation="VSHMGFVLLGLAAMNADGFRGALLQMFNHGTITGGLFLLVGVLY
                     DRAHTRDLNDFGGLGAKMPVYAGILTFFSLASLGLPGLSGFISEFLSLLGAYQYSKLI
                     TALSAIGILLAAAYLLYMIQRVLLGKLNPKWAALPDMNALELSTLVPLMVFIVAIGLY
                     PKIILEYMIPTLDGLLGTIRGGI"
BASE COUNT          786 a          873 c         1034 g          497 t
ORIGIN      
        1 ccggggaccg ctccggtaaa gcgcggaaag caaatctttc tcgtgcaccg cctggataag
       61 gaggtctcgg gccttttgat gttcgccaaa acggccggga tgaaggaaat cctgcgggcg
      121 aactggccgg acgtgacaaa acgctattat gccgttgtgg aaggcgttcc agaaaagccg
      181 gaggggactc tcaaaagcta tctgcgggag aacaagttcc tgaaggtcta cagcacgccc
      241 gcgaccgagg gcgcgaagct ttccgtgacg cattatcgcg tgatgcgctc aagcccccgg
      301 cactcgctcc tcgaggtgga gcttggtacg gggcggaagc accagattcg cgtccagctc
      361 tccgacatgg gacatccgat tgccggtgac agccagtacg gcgccaggac cgatcccgcg
      421 aagcggatcg cgctccattc gcactatctc gccttcgtgc atcccgtgac gggcaaggag
      481 acggagatcg atctcccggt cccggccgcg ttcgagaaaa tagtgactta aaaaaccctc
      541 ggaaggaaaa agggtatata cttaccggcg ccgcaggcat tattcagatg tccgtttgat
      601 ctgaacgata ctatccagga cttcaacaga acaagggagc atccatgaac aagggaaacg
      661 aacaggctgg cgggaaccag gaagaagaat tcggggaacg ggaagaagac cgggaagatg
      721 aaatggaaga ggtcgacatc accgaggtcc tcgagaagat caacgaacgg ctcgagattc
      781 tcgagaaaaa actcaatttc ctgatcgaaa ggtcagagaa cgctgggaac cgcgcggaag
      841 gcaaacaaga ttcccgtggc tttcgggagc accgcaagcc cttcggccgt ccgttccgcg
      901 gcgggggtgg cggtttcaag ccccgtcatg gcggcggagg attccatacc ggtcgtcgcg
      961 acgaccagga cgggggattc ggcaggcatc acgaaagtcc cgaaaggtcg ttcggccgtt
     1021 ctcacggagc cgcgggaagt ttccgcaaaa aacgtttcgg aagaggcggt ggacaccgca
     1081 agggatcgag aggctcgcgc gagttctagg agaagtttgg gtaaaaaggc cgggtgccct
     1141 tatcaggcgc ccggcctttt ttgctttgcg gagatttcgg cggtgaataa attcggggtg
     1201 tgaccccgat tttaattccg ggcttatccg aggatgagct tgatgaagaa accggggaaa
     1261 aggccgatgc cgaggacgcc ggcggccatg agccatgtca cggcggcggc ggagacgggc
     1321 ggtttcggga gcgcgggctg atcggcgggc acgaggtaca tggtccggat gacacggaaa
     1381 tagtaatagg ccgcgacggc actattgacg gccgcgacga cgacgagggt gatgaaccgg
     1441 gcctcgacgg cggcggcaaa aacgtaatat ttcgcgagga atcccgcgag cggcgggatg
     1501 ccggtcaggg agagcaggaa gacggtcatg agggccgccg acaccggtgc gcgctcggaa
     1561 agccccgaat aggaggcgat ctcatcgctc ccggtccggt cgccgacgga tgacaccacc
     1621 gcgaacgccc cgaggttcgt gaaaagatag gcgaaaagat atatgaggac cccgtccttg
     1681 ccgagcggcg aagggaacac ggcgaggccc atcagaatat agcccgcctg cgcgatgctc
     1741 gaataggcga ggaagcgctt gatgttcgtt tgcgagatgg ccgtgaggtt cccgagcgtc
     1801 attgtcagga tcgagagcca cataaggacc tgtcgccaat cccgatacga ctctccgaaa
     1861 acggacgcga gcacgcgcat cagcaccgcg aagccgagcg ccttgggagc gaccgtgagg
     1921 aacgccgtga ccggggtggg cgcgccctcg tagacgtccg gcgcccacat gtggaacggg
     1981 gccatcgaaa tcttgaagcc gagccccaca aaaaaacaaa ggagcccgat catgccgagg
     2041 ggtttcatcg ccggggaatc caccacccgc gcgatcgcgg gcaggtcgat cgagcccgcc
     2101 gcgccgaaaa gaagcgacat cccgtagagc atgacggccg aacaaacgct tccgaaaagg
     2161 agatatttca ccgccgattc cttcgccttt aagtcgtttt tgaggaatcc cgcgagcagg
     2221 taggaaagaa tggacacgaa ctcgaccgaa aggaagatca tgagcagatt gttagccgcg
     2281 gccaccgtga tgagcgcgaa cgccatgaaa aggaacaggc cgtagaactc gccttcataa
     2341 cggtcccgga gcggggagaa gccgaaggcg atgaggatcg tggccgcgac cgcgatcagg
     2401 gccaggaccc ggaacaggtt cgcgaacccg tcgagatgga aaaatccgta aaaaagatcg
     2461 agggactctt tcgggggcgc cttcaagaga caggccgccg cgacgatcgt gaggagcgcg
     2521 aaatatccca cgaggcgctt gttcttcagc aggaaatcca gaaagaacgc cgtaaaggcg
     2581 ccgcctaaaa ggacaagttc gggaatgaag tgccgcaggc tttcgacggg tcccacctag
     2641 atgccccccc tgatggttcc gagaagtccg tcaagtgtcg ggatcatgta ttcaagaatg
     2701 atcttcggat aaagaccgat cgcgacgatg aagaccatga gcgggacaag cgtcgaaagc
     2761 tcgagcgcgt tcatgtccgg aagcgcggcc cacttcggat tcagttttcc gagcagaacc
     2821 ctctggatca tgtaaaggag atacgccgcc gcgaggagga tgccgatcgc tgaaagggcg
     2881 gtgatgagct tcgagtactg gtacgccccg agcagcgaaa gaaactcgct gatgaaaccg
     2941 gaaagccccg ggagcccgag cgacgcgagc gagaagaagg tcagaatccc ggcgtagacc
     3001 ggcattttcg cgccaaggcc gccgaagtcg ttcaggtcac gggtgtgcgc gcggtcatag
     3061 agcacgccca cgagcaggaa gagcccgccc gtgatcgtcc cgtgattgaa catctgaagc
     3121 aaggctcccc ggaagccgtc cgcgttcatg gcggcgagac cgagcaggac gaaccccatg
     3181 tggctcacgc
//
LOCUS       DAOOBX010000075         3165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_33936_length_3165_cov_3.968810, whole genome shotgun sequence.
ACCESSION   DAOOBX010000075 DAOOBX010000000
VERSION     DAOOBX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33936_length_3165_cov_3.968810"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLY30_01675"
     CDS             <1..839
                     /locus_tag="PLY30_01675"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPW64842.1"
                     /translation="GQTPEGEITTLGRGGSDLTAVALAVALKAKHCEIYTDVDGIYTA
                     DPRVIPEAKKIDVATYDEILELASLGAKVMHSRSIEVGKKFNMPIWVCNSCKDVKGTL
                     ITKENKAMEEVVVSGVALADDEAKITLFRVADRPGVAAKVFQTLADANINVDMIIQNK
                     TKTHSTDISFTVFKNELQKALKVVKDNAKKLGVGEVASDENIAKVSVVGIGMRSHSGI
                     ASRMFSVLAENRINIEMISTSEIKISVVVAGDKGKLAARKIHKAFGLGKKQRRSKVRV
                     KR"
     gene            956..2521
                     /gene="cimA"
                     /locus_tag="PLY30_01680"
     CDS             956..2521
                     /gene="cimA"
                     /locus_tag="PLY30_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPW64843.1"
                     /translation="MKKKKILFYDTTLRDGSQAEGISLSSNDKVLIAKRLDQFGMHYI
                     EGGWPGSNPKDMEFFLAVKKAGLRRSRVAAFGSTRRAHTKVQSDPQIKALLDAGTRVV
                     TVFGKSWDLHVTDVFRTSLKENLLMIYETIAYLKDKKIEVIYDAEHFFDGFKANPEYA
                     LKTLQHAARAGADNLTLCDTNGGALPHEVRHAILRVKRAVRAPLGIHCHNDSELAVAN
                     SIQAVGGGCVLVQGTVNGFGERCGNANLISIIPDLQLKMGIRCFTPAKLKELTALSHY
                     VAEICNLPPMNNQPFTGRSAFAHKGGVHINAMIKNAKTYEHVRPDAVGNYRRYLVSEL
                     GGKTNIMIKALELEMELQKDSPETKKILAEIQRLEYEGYQFEAAEASFELLVQRVLGR
                     FKPFFTVNDISVRTENIGNGRPRSIANVSVIAKGAEHVESAEEDGPVNALDSALRKAL
                     AKTYPVISEIHLTDYKVRVVNSEAGTAARVRVFIEFQDKRKTWTTVGVHENIIEASWK
                     ALVEAIEYKLHKG"
     gene            2588..>3165
                     /locus_tag="PLY30_01685"
     CDS             2588..>3165
                     /locus_tag="PLY30_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPW64844.1"
                     /translation="MDLDKQYNSKEVEQKWYRIWEEKGYFRPRSDGKWCRKSSGFPEP
                     KPGSDSACQRPDLYREGLPPYVIVIPPPNVTGILHMGHALNNSIQDIMIRWNRMKGND
                     TLWVPGVDHAGIATQNVVEKKLAKEKKTRWDLGREKFLEEVWAWKDAHGSTITNQLRR
                     LVASCDWTRERFTMDEGLSKAVRAAFVTLYDRG"
BASE COUNT          746 a          865 c          980 g          574 t
ORIGIN      
        1 agggtcagac cccggaaggg gagatcacaa cgcttggccg cggcggttcc gatcttacgg
       61 ccgtggcgct cgcggtggcc ctcaaggcaa agcactgcga gatctatacg gacgtggacg
      121 ggatttatac ggccgatccg cgcgtgattc cggaagcgaa aaaaatagac gtcgcgacct
      181 acgatgaaat cctcgagctt gcgtcgctcg gagcgaaggt catgcactcg cggtcgatcg
      241 aggtcggcaa gaaattcaac atgcccattt gggtgtgcaa ttcctgcaag gatgtcaaag
      301 gaactcttat tacgaaggag aacaaggcca tggaagaagt cgttgtgagc ggtgtcgcgt
      361 tagctgatga tgaagccaag atcaccctgt tccgcgtcgc ggaccgtccg ggtgttgcgg
      421 cgaaggtgtt ccagaccctc gcggacgcga acatcaacgt ggatatgatt atccagaaca
      481 agacgaagac gcattcgacc gacatctcgt tcaccgtttt caagaacgag ctccagaagg
      541 ccctcaaggt ggtcaaggac aacgcgaaga agctcggcgt cggtgaggtc gcgagcgacg
      601 agaatatcgc gaaggtcagc gtggtcggga tcgggatgcg gtcgcattcc ggtatcgcgt
      661 ccaggatgtt ctccgtgctc gcggaaaaca ggatcaacat cgagatgatc tccacctccg
      721 agatcaagat ttcggtggtg gtggcgggcg acaaaggcaa gctcgcggcg cggaaaatcc
      781 acaaggcctt cgggctcggc aagaaacaac ggcgttcaaa ggtgagagtg aagcggtaag
      841 tcccgcgccg ggcgcttatg gcagccgggg atcatgggaa aaattctttt ttgcggcgca
      901 acattgcggg gatgctccgg tttgcgcagc cgaaggaatc ggtaacagga tgaagatgaa
      961 gaagaaaaaa atattgtttt atgacacgac tttacgggat ggatcccagg cggaagggat
     1021 cagtctgtcc tcgaatgaca aggtgctgat cgcgaagcgg ctcgatcagt tcgggatgca
     1081 ctatatcgaa ggcggatggc cgggttccaa cccgaaggac atggagttct ttctggcggt
     1141 caaaaaggcc gggctccggc ggtccagggt cgccgcgttc ggttccacgc gccgcgccca
     1201 cacgaaggtc cagtcagacc cgcagatcaa ggccctcctc gatgccggga ccagggtcgt
     1261 gacggtgttc gggaaatcct gggacctgca cgtgacggac gtgttcagga cttcgctcaa
     1321 agagaacctc ctgatgatct acgagaccat cgcgtacctg aaggacaaaa agatcgaagt
     1381 gatctacgac gcggaacatt ttttcgacgg cttcaaggcg aatcccgaat acgcgttgaa
     1441 gaccttgcag cacgcggcca gggccggcgc ggacaacctg acgctttgcg acacgaacgg
     1501 cggcgcgttg ccgcacgagg tccggcatgc catcctgagg gtgaagcgcg ccgtcagggc
     1561 gcccctcggt atccattgcc acaatgattc cgaactcgcg gtcgcgaatt cgatccaggc
     1621 cgtgggcgga ggctgcgttc tcgtgcaagg cacggtgaac gggttcggcg agcgttgcgg
     1681 gaacgcgaat ctcataagca tcatcccgga tctgcagctc aagatgggga tccgctgctt
     1741 tacgccggcg aaactcaagg aactgaccgc gctttcgcat tatgtggcgg agatctgcaa
     1801 tctgccgccc atgaacaatc agccgttcac gggacgttcc gcgttcgcgc ataagggcgg
     1861 ggttcacatc aacgcgatga tcaagaacgc gaagacctat gagcacgtga ggcccgacgc
     1921 ggtcggcaac taccggcgct atctcgtctc ggaactcggc gggaagacga acatcatgat
     1981 caaggcgctg gaactcgaaa tggagctcca gaaggattcg cccgagacga agaaaatcct
     2041 cgccgaaatc cagcgcctcg aatacgaggg atatcagttc gaggccgcgg aagcgagttt
     2101 tgagcttctc gttcagcgcg ttctcggaag gttcaagccg ttcttcacgg tcaacgatat
     2161 ctcggtccgc accgaaaaca tcgggaacgg tcgtccgcgt tcgatcgcga atgtgagcgt
     2221 gatcgccaag ggcgccgagc atgtggaatc cgcggaagag gatggccccg ttaacgcgct
     2281 cgattccgcg ctccgcaagg ccctcgcgaa gacctatccc gtgatcagcg agatccacct
     2341 caccgactat aaagtgcgtg tcgtgaactc cgaggcggga accgccgcga gggtgcgggt
     2401 cttcatcgag ttccaggaca aacggaagac ctggaccacg gtcggcgttc acgagaatat
     2461 catcgaggcg agctggaagg ccctcgtcga agcgatcgag tataaattac acaaaggctg
     2521 atgaggagcg cggtgcgcgg cgtaaaagtc ttttctccgc gccccgtatc ccgaacttcg
     2581 tattaatatg gacctggaca aacaatacaa ttcgaaagaa gtcgagcaga agtggtaccg
     2641 gatctgggaa gagaagggct acttccgtcc gagaagcgac ggaaaatggt gccggaaatc
     2701 ttccggcttc cccgagccca agccgggctc tgacagcgcg tgccaaaggc cggatttata
     2761 ccgggaagga ctcccgccgt atgtcatagt cataccgccg ccgaacgtta cgggcattct
     2821 ccacatgggt cacgcgctca acaactccat ccaggacatc atgatccgct ggaaccggat
     2881 gaaggggaac gacacgctct gggttccggg cgtggaccac gcgggcatcg cgacccagaa
     2941 cgttgttgag aaaaagctcg cgaaggaaaa gaagacccgc tgggatctcg gacgtgagaa
     3001 attcctcgaa gaggtgtggg cttggaagga cgcccacggt tcgaccatca cgaatcagct
     3061 ccgtcgtctc gtcgcttcct gcgactggac ccgcgagcgg ttcacgatgg acgaaggtct
     3121 ttcgaaggcc gtccgcgcag ccttcgtcac gctctatgac cgcgg
//
LOCUS       DAOOBX010000076         3150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_34149_length_3150_cov_2.737964, whole genome shotgun sequence.
ACCESSION   DAOOBX010000076 DAOOBX010000000
VERSION     DAOOBX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34149_length_3150_cov_2.737964"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLY30_01690"
     CDS             complement(<1..454)
                     /locus_tag="PLY30_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64845.1"
                     /translation="MITINLLPAEYRTAKRRGTTARPYIPIAILAAALLLLLTLFFYA
                     DFLKVKSSYERVRKEWIRFAPQMKHLKDLEQKVDVAMKGEKEFLESSILNSDPFTRVL
                     QLVSERLPKGAWLTELKIERLKEGGNSVLLQGAVVPVGAKTGIEQIEEF"
     gene            complement(451..1551)
                     /gene="pilM"
                     /locus_tag="PLY30_01695"
     CDS             complement(451..1551)
                     /gene="pilM"
                     /locus_tag="PLY30_01695"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPW64846.1"
                     /translation="MKSDLLTIKNIFGGATALFRSAFGSWLPKGPSAPRATVCDLGRS
                     KIVLLELSRRSDGVFIEKFELIKNALQDTKPSLLLKPFFESGKFSRENIRVTLKGHGV
                     IMRFIRFPRMKPEDLRGAMQYEVEQYIPFELKDCVMDYAIIDESVKTEEGEKMEVLLA
                     VVKRQEVEPTVEVFRNLECKLSVIDVDILSAMSALECFYPEDAAGHSAILDIGTEIST
                     LGIIREGKPRFIRDISYGTYDVHKRLRSKTSLSDEEIDTLFDRNAEEKPDVAVAISES
                     LDGFIGDLKVSFDYYRDQSRVAGHIGKLFVSGVGSLHPVVIGTLSEGLGIPVLGMDVL
                     PKLKWADTVDGEQLKKHQSLLPVALGLGLRSE"
     gene            complement(1643..2098)
                     /locus_tag="PLY30_01700"
     CDS             complement(1643..2098)
                     /locus_tag="PLY30_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPW64847.1"
                     /translation="MFIKLNQRGFTLVEIMIVVAIIGLLAAIAIPNLLRARINANEGA
                     IKSDLRTFSSAAESYRAAQNPPTYPSAITDMTGATPAYLDTSWTGNKHGFGFTYNSTS
                     ADTFTLLATPASSSTAVNIYCVDQTGVIQSGAAGVSADGTACSGGTAIA"
     gene            complement(2273..>3150)
                     /locus_tag="PLY30_01705"
     CDS             complement(2273..>3150)
                     /locus_tag="PLY30_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPW64848.1"
                     /translation="GKSLSESMLPHQKVFSALFIHMVRAGEQSGRLEEIMDRVAQYFE
                     KASALQKKVKSAMMYPLVVSIMAFGITFAMLAFIIPKFATIFDGLGAKLPAPTLFLIK
                     ISNYMAANWWWILGVIGGLVFACRQIVRLPAGRLAWDSFQLRMPIFGVIILKVAISKF
                     SRTLATLIKSGVAIISALEIVTKTSGNARIEHMIIELMAAVKRGENIAGPLERGGVFP
                     QMVIRMIAIGEETGELEGMLTKIADFYDTQVDTAVAGLTSLIEPLIIAFLGIVIGGIV
                     IALFLPILTLTQAMK"
BASE COUNT          728 a          934 c          821 g          667 t
ORIGIN      
        1 caaactcctc gatctgctcg atccccgttt tggctccgac ggggacaacc gctccctgca
       61 gcagcacgga atttccgcct tccttcaatc tttcaatctt cagctccgtc agccaggccc
      121 ccttgggcag ccgctcgctg accagctgga gaaccctcgt gaacggatcg gaattgagga
      181 tactgctctc caggaattcc ttttcccctt tcattgcgac atccactttt tgttcaaggt
      241 ccttgagatg cttcatctgc ggcgcgaacc ggatccactc cttgcggaca cgttcgtagg
      301 agctcttgac cttcaggaaa tccgcgtaga aaaagagcgt cagtaaaaga agcaatgccg
      361 cggcaaggat cgcgatgggg atgtagggac gcgccgtcgt gccccgccgt ttcgcggtgc
      421 ggtattccgc gggaagaaga ttgatcgtaa tcattccgac ctcagtccga gcccgagagc
      481 aaccggtaaa agggattggt gctttttaag ctgttcgcca tcgaccgtgt ccgcccactt
      541 gagcttcggg agaacatcca tgcccaggac cgggatgcca agcccttcgc tcagcgtccc
      601 gatcaccacg gggtgcaacg acccgacccc gctcacaaag agcttcccga tatgcccggc
      661 aacgcgggac tggtcccggt aataatcgaa ggagaccttc aggtccccga tgaaaccgtc
      721 aaggctttcc gagatcgcaa cggccacgtc cggcttctct tccgcgttcc tgtcgaacaa
      781 agtgtcgatc tcttcgtccg aaaggctcgt cttggaacgc aaacgcttgt gcacatcgta
      841 cgtcccgtag gaaatgtcac ggataaaacg aggcttgcct tcccgtatga tcccgagcgt
      901 cgagatctcg gtcccgatat ccaggatcgc cgaatgtccc gcggcatcct ccggataaaa
      961 acattcgagc gcggacatgg ccgacaggat atccacgtcg atcacggaaa gtttgcattc
     1021 cagattgcgg aagacctcca ccgtcggctc cacctcctgg cgtttgacga ccgcgagcag
     1081 gacctccatc ttctcgccct cctccgtctt cacgctctcg tcgatgatcg cgtagtccat
     1141 cacgcagtcc ttgagctcaa acggaatata ctgctccacc tcatactgca tcgcgccgcg
     1201 caggtcctcg ggcttcatcc tggggaaccg gatgaaacgc atgatcacgc catgtccctt
     1261 gagcgtgacc cggatattct cccgggagaa cttcccggat tcaaagaaag gcttcagaag
     1321 aagggacggc ttcgtgtcct ggagggcatt tttgatcagc tcgaatttct cgataaaaac
     1381 gccatcggac cttctggaaa gctcgagcaa aacgatcttg gaacggccga gatcgcagac
     1441 agtcgcgcgc ggggccgaag gccctttcgg aagccaggac ccgaacgccg aacgaaaaag
     1501 agccgtcgcg ccgccaaaga tattcttgat ggttaaaagg tcggatttca tgggttgcat
     1561 cataccacaa gtgacaataa aaaacccacc ctgcttttaa cggcagggtg ggttttcgaa
     1621 cacttaaaat actttccgtt acctaggcga tcgccgtacc accgctgcag gcagtgccat
     1681 cggcggacac acccgccgcc ccactctgga tcacgcccgt ctggtcaacg caatatatat
     1741 tgaccgcggt cgagctcgag gctggtgtcg cgagaagcgt gaatgtatca gccgaagtac
     1801 tgttgtaggt aaagccgaaa ccatgtttgt tgccagtcca cgacgtatcg aggtacgcag
     1861 gcgtcgcacc ggtcatgtcg gtgatggccg acggatacgt gggcgggttc tgggcggcgc
     1921 gataactctc ggccgcggaa ctgaacgtcc tcagatcgga tttaatagca ccctcgttgg
     1981 catttatcct ggcgcgaaga agattgggga tagcgatagc cgccaaaagc ccaatgatcg
     2041 caaccacgat catgatttcc accaatgtga aaccacgttg attcagctta atgaacatat
     2101 actacctcct gtttatttac catagcctgc taatagtgta tctcacactt ggatattcgg
     2161 caatgattta tagcaacttg agtctttttt atcatgccgg aggaagactt gctgaaaatc
     2221 tttaaaaaga cgcccgccat atacaaaagc cttcggacac atactcaaaa gattatttca
     2281 tcgcctgcgt cagggtcagg atcggaagga acaacgcgat gacgatgccg ccgatcacaa
     2341 tgccgaggaa cgcgataatc aacggctcaa tcagggaggt cagacccgcg accgcggtgt
     2401 caacctgggt atcatagaag tccgcgatct tcgtgagcat cccttccagt tcaccggtct
     2461 cttcgccgat cgcgatcatg cggatgacca tctgggggaa aacccctccc cgttccagcg
     2521 gccccgcgat attttcaccg cgtttgacgg ccgccatgag ctcgatgatc atatgctcga
     2581 ttctcgcgtt gccggaggtc ttggtgacga tctcgagcgc ggaaatgatc gcaacgccgc
     2641 tcttgatgag cgtcgcgagg gtccttgaga atttgctgat cgctaccttc aggataatca
     2701 ccccgaagat cggcatgcga agctggaagg aatcccaggc cagacgcccc gcgggaagcc
     2761 ggacgatctg ccgacacgcg aaaacgagcc ccccgataac accgaggatc caccaccagt
     2821 tcgcggccat gtaattactg atcttgatga ggaacagcgt cggggcgggc agtttcgcgc
     2881 cgagaccgtc aaagatcgtc gcgaatttcg gaatgatgaa cgcgagcatc gcgaacgtga
     2941 tcccgaaggc catgatcgac acaaccagcg ggtacatcat cgccgacttg accttcttct
     3001 gcagggcgct cgccttttca aaatactggg ccacgcggtc catgatctcc tcgagtcgtc
     3061 cgctctgttc ccccgcccga accatatgga taaaaagggc gctgaagacc ttctgatgcg
     3121 gcaacatcga ctcggaaagg cttttccccg
//
LOCUS       DAOOBX010000077         3143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_34256_length_3143_cov_2.647992, whole genome shotgun sequence.
ACCESSION   DAOOBX010000077 DAOOBX010000000
VERSION     DAOOBX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34256_length_3143_cov_2.647992"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLY30_01710"
     CDS             <1..625
                     /locus_tag="PLY30_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPW64849.1"
                     /translation="GTLAIHATRPNVFTHDDLRLLDAIATLASSAIANALLYEKTEQL
                     AIKDSLTGLFVRRYFYERLKEEHRRALISQKTMSLIMCDLDHFKECNDRFGHQGGDLM
                     LIQLAEILSENLKSALLARYDGEEFAILLPETSKAEALKVAEGIRSVVENAPFNIRRE
                     KVSMTISAGVANLPDDTLDLESLVHKADQALYKAKREGRNKVCAAAS"
     gene            607..1992
                     /locus_tag="PLY30_01715"
     CDS             607..1992
                     /locus_tag="PLY30_01715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW64850.1"
                     /translation="MRGSLLILAAQLVLGFFILCISKELVLPYYLLAPLMLLPSFYFY
                     RDSARKSLLVSLFVAVLFNLRFVFVDPHPRFFSFLLLQALFFIVLCLYSRELRETIRQ
                     ADEKKNEVRKNLDNLQAKFQTRLTSLKHLELQVSSLMNLFEIARDFSECMDFATLTEF
                     LLKKLRDELSFERMRIFLFPAPTDPVEPGTVKVYDMVANGVTETERVLSDQEKTDFGR
                     MRAEKNMVKNDSSWHFPIFEAAELGAVFSIEGVKDEDLARVEVLSAYLVLLIKKIKLY
                     ETVRKLAIVDELTQVFVRHHFSERLEEEVERSVRFRLPLAVLMLDIDHFKRYNDDFGH
                     LVGDATLKEVADLLKKSLRRVDLVGRYGGEEFLVAMPETKLENAVEVAERIRSSIARH
                     DFQVYNDKTRVTVSQGLVVFDAAKLANPERADFSKIAAELVHQADEAMYQAKDEGRNR
                     VCIYKESKKTL"
     gene            1989..>3143
                     /locus_tag="PLY30_01720"
     CDS             1989..>3143
                     /locus_tag="PLY30_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64851.1"
                     /translation="MKFDIFSRNTIVAFIILSLTAVPAASAQDSRSAEKAEDPGAAPT
                     KTSSSRPVPFKTVFDQSQAKVEAVADSLEYQKDSNKLVARGNAVLTYQNMKLLADYAE
                     VESNTKKAYAKGHVLVFDGDKPFMSGDEVYYDFANKTGSFPKGRIFSEPLFARGDETQ
                     QVKPREFKVKDGGVTTCNLENPHYEIRCKKATLFQGVKVRMVNVTIYVLGKPVFWLPY
                     LTIPLNWNVPFQATAGYRKEYGAYIELTKGITFNEHLSGKWHADWRSKRGFGGGWDQF
                     YEFGKYAKGDVKLYWTQDKRAPTPRHQDIDGKDDPYGERQKRDRGRITWRHRTDFDDY
                     TNIIMRYHRADDPYVLQDFFEDEYRSEMEPHSFVTATRNTQKWGAMVHVEK"
BASE COUNT          707 a          869 c          885 g          682 t
ORIGIN      
        1 cggaaccctc gcgatccatg cgacgcgccc caatgttttc acgcatgatg acctccggct
       61 cctcgatgcg atcgcgaccc tcgcttcctc ggccatcgcg aacgcgcttc tctatgagaa
      121 gaccgaacag ctcgccatca aggattccct gaccgggctg tttgtgcggc ggtatttcta
      181 cgaacgcctc aaggaagagc accgccgcgc gctgatatcg cagaagacca tgtctcttat
      241 catgtgcgac ctcgaccatt tcaaggagtg taatgacagg ttcggccacc agggcggcga
      301 cctcatgttg atccagctcg cggagatcct tagtgagaac ctgaagagcg cgctgctcgc
      361 gcgttacgac ggtgaggagt tcgcgatcct tcttccggag acctcgaagg ccgaggctct
      421 caaggtcgcg gagggaattc gctcggtcgt ggagaacgcc ccgtttaata tccgccgtga
      481 aaaggtcagc atgacgatct cggcgggggt tgcgaacctt cccgatgata cgctggacct
      541 ggagtcgctc gtccacaagg ccgatcaggc gctttacaaa gcgaagcggg aagggaggaa
      601 caaggtatgc gcggcagcct cctgattctc gccgcccagc ttgtcctggg tttcttcatc
      661 ctgtgcattt cgaaggagct ggtcctcccg tattatctgc tggctccgct catgcttttg
      721 ccgtcatttt atttttacag ggattcggcc cgcaagtcgc tcctcgtatc gcttttcgtg
      781 gcagttctgt tcaacctccg gtttgttttt gtcgacccgc acccgcggtt cttctcgttc
      841 ctgctgctgc aggccctttt cttcatcgtc ctttgcctgt actcccggga gcttcgcgaa
      901 acgatccgtc aggcggatga gaagaagaac gaagtccgca agaatctgga caacctccag
      961 gcaaaattcc agacacgcct cacgagtctg aaacatctcg agctccaggt gagcagcctc
     1021 atgaatcttt ttgagatcgc ccgggatttc agcgaatgca tggatttcgc gacgttgacg
     1081 gaattcctgc tcaagaaact gcgcgacgaa ctttcgttcg agcgcatgcg catctttctc
     1141 tttcccgccc cgaccgaccc ggtcgagccg ggcacggtca aggtctacga catggttgcg
     1201 aacggagtga ccgagaccga acgtgtgctg agcgatcagg aaaaaacgga tttcgggcgc
     1261 atgcgcgccg aaaaaaacat ggtgaagaac gacagttcct ggcatttccc gatcttcgag
     1321 gcggcggagc ttggcgccgt tttctcaatt gagggagtca aggacgagga tctcgcgcgg
     1381 gtcgaggtgt tgagcgccta tctcgttctc ctgatcaaga agatcaagct gtatgagacc
     1441 gtccggaagc tcgcgatcgt ggatgaactc acccaggtgt tcgtccgcca tcacttttcc
     1501 gaacgtctgg aggaagaggt tgagcgtagc gtgcgtttca ggctccctct cgcggtatta
     1561 atgttagaca ttgatcattt caaaagatat aatgacgatt tcggacattt ggtcggggac
     1621 gccacattga aagaagtggc ggatctcctc aaaaaaagcc tccgtcgcgt ggacctggtg
     1681 ggccgctacg gcggggaaga gttccttgtt gcgatgccgg aaacgaagct cgagaatgcc
     1741 gtcgaagtcg cggaacgtat ccggtccagc atcgcgcgtc acgattttca ggtctataac
     1801 gataaaacac gcgtcacggt cagccagggt ctcgtggtgt tcgacgccgc gaagctcgcg
     1861 aatcccgaac gggcggattt ttcgaagatc gccgccgagt tggtacacca agcggatgag
     1921 gcgatgtacc aagccaagga tgaaggtcgt aaccgcgtct gcatttataa ggaatcgaaa
     1981 aaaacgttat gaaattcgat atcttcagcc ggaatacgat cgtagcattt atcattcttt
     2041 ccctgacagc cgttcccgcc gcttccgcgc aggattcccg gtccgcggaa aaggccgagg
     2101 atccaggtgc ggccccgacg aagacatctt cctcccggcc cgttccgttc aagaccgtat
     2161 ttgatcagtc ccaggccaag gtcgaggcgg tcgccgacag cctcgaatac cagaaagact
     2221 ctaacaaact ggttgcgcgc gggaacgccg tcctgacgta ccagaatatg aaacttctcg
     2281 cggattacgc cgaagtggaa agcaacacca aaaaggctta cgcgaaaggt cacgtgcttg
     2341 tctttgatgg agataaaccc ttcatgagcg gcgacgaagt ttattacgac ttcgcgaaca
     2401 agacgggcag tttccccaaa gggcggattt tcagcgagcc tttattcgcg cgcggcgatg
     2461 agacccaaca ggtgaaaccc cgggagttca aggttaagga cggcggggtg acgacctgta
     2521 atcttgaaaa tccgcattat gaaattcgct gcaaaaaggc cactcttttc cagggcgtga
     2581 aagtccggat ggtcaacgtg acgatctatg tgcttggcaa gccggtgttc tggctccctt
     2641 acctgacgat tccgctgaat tggaacgtgc cgttccaggc gaccgcaggt taccggaaag
     2701 agtacggcgc gtatatcgaa cttacgaagg ggatcacctt taatgaacac ttgtccggga
     2761 aatggcacgc cgactggcgc tccaaacgcg gcttcggcgg gggatgggac cagttctatg
     2821 aattcggcaa atatgccaag ggcgacgtca agctctactg gacgcaggac aagcgtgctc
     2881 ccactcccag gcatcaggac atcgatggca aggatgatcc ttatggcgaa cgccagaaac
     2941 gtgaccgcgg ccggatcacg tggcggcacc ggacggattt tgacgattat acgaatatca
     3001 tcatgcgcta tcaccgcgct gatgaccctt atgtcctcca ggatttcttc gaggatgaat
     3061 accgctccga gatggagccg cattcgttcg tgacggcaac gcgcaacacc cagaagtggg
     3121 gtgccatggt ccacgtcgag aag
//
LOCUS       DAOOBX010000078         3111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_34750_length_3111_cov_3.179647, whole genome shotgun sequence.
ACCESSION   DAOOBX010000078 DAOOBX010000000
VERSION     DAOOBX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34750_length_3111_cov_3.179647"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PLY30_01725"
     CDS             complement(<1..867)
                     /locus_tag="PLY30_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPW64852.1"
                     /translation="MRVEKRTPVAAIYEKAYYLYQIRILAILALISLYIFTKRWVGSE
                     ANLWPLLGVILIEIFVNRPYKFLFTDSAAGYRVLLWSVVIDFAAETTALHLLGEMDLF
                     IYGICYFISIVYCALNIRASLSFAFATLASAIHIVLLVLEHFHVIPATVSFAGQAVTW
                     SEQLSMTVRYIAFFYLLAIFVRFMGKALIQKEEKLEDLIRELKSTNEETKYAYHLQTD
                     YFARMSHEIRAPLNSILGFSQLLLENATGPLSDKQRDFITRIERGSRHLQDMINDVLD
                     LSKIESKKMSLTL"
     gene            1026..2132
                     /locus_tag="PLY30_01730"
     CDS             1026..2132
                     /locus_tag="PLY30_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPW64853.1"
                     /translation="MSERALLMSVLEYAIDLKGQDVFFKADTAPRMRLGHLVTPLPFP
                     PVTGERIEAMSREILTPKQLEGLTKNKSIDFGLTHSGKGCRFRGNIFYQQGTLSAVFR
                     LLWKGVPDFSQLALPPILQKVALEKAGIILIGGTVSSGKTTTIAAMIHTMNENAQKHV
                     ITIEDPIEYLHEDKQCLIQQREVGEDTESFASAMKYIVRQSPDVVVIGEMRDAESFSF
                     ALASAEVGRLVISTVHAQSVGQVFDRVLGFFAPEHREAVLRHFAMNVSCIAVQKLLVG
                     ADGKSLVPAVEIMLGSYIIKQLIMEHRVDKLAQALRNSSQEGMQTMDQSLLQLWEKKL
                     ITRETALASSQSPQELEAWMKGIKIGQDTKILGG"
     gene            complement(2215..>3111)
                     /locus_tag="PLY30_01735"
     CDS             complement(2215..>3111)
                     /locus_tag="PLY30_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPW64854.1"
                     /translation="RFPKHGTLTATIMTFLVFILIILPLVMILINVTAQAIETSQGIY
                     NYVRQGKLEQLIENIRATGWIRKVETKIVQWDMLKQNLTSWLLVATEKIGNFTASQVG
                     TFTKNILFFLLNFFFMFFLTFVFFKHGAKIYEFIYQIAPFEKETKDTIFRQINDTFTA
                     VIRGQILTSFAQSVVAGITFWILGIPVPVLFAFLLFITSMIPVLGAASIWLPLVIYLA
                     LSGFIIKAIILAGVGLLGISLLDNIIKPAIIGERTKLPYFLLFFGIMGGLKFFGLMGI
                     FLAPVVLSVFFALIRIYRQQYL"
BASE COUNT          745 a          782 c          933 g          651 t
ORIGIN      
        1 gagcgtgagg ctcattttct tggattcgat cttggaaagg tccaacacgt cattgatcat
       61 gtcctggagg tggcggctac cgcgttcgat gcgcgtgata aagtcccgct gcttgtcgga
      121 aagaggaccc gttgcgttct cgagcaggag ttgcgagaaa ccgaggatgg agttcaaggg
      181 cgcccggatc tcgtggctca tgcgcgcgaa ataatcagtc tgaaggtggt atgcgtattt
      241 cgtttcttcg tttgtgctct tgagctcccg gatcaggtcc tcgagctttt cctctttctg
      301 tatcagcgcc tttcccatga accgcacgaa aatcgcgaga agataaaaaa aggcgatata
      361 ccgtaccgtc atgctgagtt gctcgctcca ggtgacggcc tgtccggcga aggatacggt
      421 agcggggatt acatggaaat gctccaagac gagcaacacg atatggattg ccgaggccaa
      481 ggtcgcgaag gcgaagctca gggacgcgcg gatgttgagg gcgcaataga cgatcgagat
      541 gaaatagcag attccgtaga tgaaaaggtc catctcgccg aggaggtgca gggccgtcgt
      601 ttccgcggcg aagtcgatga cgacggacca gagaagcacc cggtaacccg cggcggaatc
      661 ggtaaagagg aacttgtagg gccggttgac aaaaatctcg atcaggatga cgccaaggag
      721 cggccagagg ttggcctccg agcccaccca ccgcttcgtg aagatataga gtgagatcag
      781 ggcaaggatc gcgagaatgc gaatctggta aagatagtag gccttctcat agatggcggc
      841 gaccggcgtt cttttttcga ccctcatgat atggagaccc tcacggaaaa atatgataat
      901 caaacaaagg cgaaaatgga atgcaatttt aaactgcctg gccgatacac ttgtttgacg
      961 gcaaagccga gtgttacaat acccccgtgt ttctaacggc tgttcatcga acagaaaggc
     1021 tgcccatgag cgaacgagct ttactgatgt ccgtgctgga gtatgcgatc gatctgaagg
     1081 ggcaggacgt gtttttcaag gcggacacgg cgccgcgcat gcggctcggt catctcgtga
     1141 cgccgcttcc gttcccgcct gtgaccgggg aaaggatcga ggcgatgagc cgcgagatcc
     1201 tgacgcccaa gcagctcgaa ggactgacga agaacaagag catcgacttc ggtttaacgc
     1261 actccggaaa gggctgccgc ttccgaggga acatctttta tcagcaggga acgctttcgg
     1321 ctgtcttccg tctcttgtgg aaaggggttc cggatttctc gcagctggcg cttcctccca
     1381 tcctgcaaaa agtggctctc gagaaggccg gcatcatcct gatcggcggc acggtctcct
     1441 ccgggaaaac gacaacgatc gccgcgatga tccatacgat gaatgaaaac gcccagaaac
     1501 atgtgatcac gatcgaggac cccatcgaat atctccatga ggacaagcag tgcctgatcc
     1561 agcagcggga ggtcggggag gatacggaga gttttgcgtc ggcgatgaaa tacattgtcc
     1621 ggcagtcccc ggacgtggtt gtgatcggag aaatgcggga tgccgaatcg ttcagttttg
     1681 cgcttgcctc ggccgaggtg ggccgtctcg tgatctctac cgtgcacgcc cagtcggtcg
     1741 ggcaagtttt tgaccgcgtg ctgggtttct ttgcccccga acaccgtgag gcggtgctgc
     1801 ggcattttgc catgaatgtc agctgtatcg cggtacaaaa gctccttgtc ggcgccgacg
     1861 ggaaaagcct cgtccctgcc gttgagatca tgctgggcag ttatattatc aaacagctca
     1921 ttatggagca cagggtcgac aagctcgcgc aggctctccg gaacagcagc caggagggaa
     1981 tgcagacgat ggaccagtca ctgcttcagc tttgggagaa gaagcttatc acgcgagaga
     2041 ccgcgctcgc gtcgagccag tctccgcaag aacttgaggc gtggatgaaa gggatcaaga
     2101 tcggccagga tacgaaaatc ctcggcggat aaacaaggcc acgcgccata cgatgcgaga
     2161 cgccggggaa ttctttttcg cgcggcgttg tgcctttacg gcttgcggcg atggttacag
     2221 gtattgctgg cggtaaatcc ggatcaacgc gaagaagacc gaaagcacga ccggagcgag
     2281 aaagattccc atcaggccga agaacttcag tccccccatg attccgaaaa acagcaggaa
     2341 atacggaagt ttcgttctct ccccgatgat cgcgggcttg atgatattgt cgagcagact
     2401 gattcccagg agtcccactc ccgccaggat gatcgccttg attatgaacc ccgacaacgc
     2461 gaggtagatc acaagcggta gccagatgct cgcggccccg aggacgggga tcatggacgt
     2521 gatgaaaagc aggaacgcga aaaggacggg cacgggaatg cccagtatcc agaaagtgat
     2581 gccggcgacc acggactgag cgaagctcgt gaggatttgt ccccggatga ccgccgtgaa
     2641 ggtgtcgttg atctgccgga agatggtgtc cttggtctct ttttcgaatg gggcgatctg
     2701 gtagatgaat tcgtagattt tagccccgtg cttgaaaaag acgaacgtca ggaagaacat
     2761 gaagaaaaag ttcagaagga aaaagaggat gttcttcgtg aaggtgccga cctgggacgc
     2821 cgtgaagttg ccgatctttt ccgtcgcgac gagtagccag gacgtcaggt tttgtttcag
     2881 catgtcccat tggacgatct tggtctccac tttccggatc cagcccgtgg cccggatatt
     2941 ttcgatcagt tgctccagtt ttccctgccg gacgtaattg taaatccctt gcgaggtctc
     3001 gatggcctgc gccgtcacat taatcaggat catcacaaga ggcaggataa tcaggataaa
     3061 gacgaggaac gtcatgatgg tcgcggtcag agtcccgtgc ttcggaaagc g
//
LOCUS       DAOOBX010000079         3111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_34754_length_3111_cov_2.694372, whole genome shotgun sequence.
ACCESSION   DAOOBX010000079 DAOOBX010000000
VERSION     DAOOBX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34754_length_3111_cov_2.694372"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLY30_01740"
     CDS             complement(<1..348)
                     /locus_tag="PLY30_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HPW64855.1"
                     /translation="MALIIKRVERPRELKWYQAGAMLYGDWGTSKAYVIGIAFALAGH
                     ASWFFLGLMSLLTVLVGFCYIVVCRIFPDGGGVYSSVKHRSQTLAVIGALLLFANFVV
                     TAALSVLDAFHYFN"
     gene            680..>3111
                     /locus_tag="PLY30_01745"
     CDS             680..>3111
                     /locus_tag="PLY30_01745"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPW64856.1"
                     /translation="MRKLFTRLSILGALCLGQTAAWASEADLKIPDLHEGRFNLFGGL
                     TGFQILLYGAIVILGTVGLSLYLFMRVKALPAHKSMLAVAETIFQTCKTYLKQQIKFL
                     TILFAIIACTMGYYFVGLKHEPMTTLGLILLFSIVGMGGSALVAYYGIRINTYANART
                     AFASLRGKPWDVVNIPMCSGMSVGLFLISLELVMMVIILLFVPRDIVGYCFLGFAIGE
                     SLGASALRIAGGIFTKIADIGSDLMKIVFKVKEDDPRNPGVIADCTGDNAGDSVGPTA
                     DGFETYGVTGVALISFITLAIKDPSLQAKLIVWIFAMRFLMDFMSGASYFINQFISER
                     KYKGLKEFNFEEPLTRLIQIASTLCITTSFGMSYLLVGDLPDPTLWWKLASIITCGTL
                     AAYLIPEFTKAFTSSHSRHVQEIVTASREGGASLTILSGLVAGYFSAFWKGILIAGLM
                     FVAYLISGMGLQNIMPHASVFAFGLVAYGFLCMGPVNIAVDSYGPVTDNAQSIFELAQ
                     TGSIPGIEQEIEKDFGFKPDFRIGKHYLEANDSAGNTFKATAKPVLIGTAVAGATTMI
                     FSIILLLQEQLHSGAIAAALGGLVPENIGETLLNAKLSLTAAPILLGILAGGAVIFWF
                     CGASIQAVTTGAYSAVEYIKKNMNLDKSEADRKDSVTVVRICTEYAQKGMWNIFLGLL
                     MLTLAFALFDPYFFIAYLIAIATFGLYKAMFMANAGGSWDNAKKVVEVDLGEKNTPVH
                     AATVIGDTVGDPFKDTSSVSLNPIIKFSTLFGMLAVEIAIKMNPATTRVSGAVLFALG
                     LVFVWRSFYKMR"
BASE COUNT          652 a          917 c          843 g          699 t
ORIGIN      
        1 gttgaaatag tggaatgcgt caagcacgct caacgcggcg gtgacgacaa aattcgcgaa
       61 gagaagcagc gcgccgatta ccgcgagggt ctgggaacga tgtttgacgg aggagtaaac
      121 acctcctccg tccggaaaga tacggcagac aacaatgtag caaaacccga caaggaccgt
      181 caaaagggac atgaggccca aaaagaacca ggaggcatgc ccggccagag cgaacgcgat
      241 accgataacg taggctttgg aggttcccca gtcgccatag agcatggcgc cggcctggta
      301 ccatttaagt tcgcgcggcc gttcgacgcg cttgatgata agtgccataa agggctccta
      361 atatagcaga agaaaaaaga caatcaatag atgccgtctc ctcccgcgag cgctttcctg
      421 cgcttacgct taaaagaaag attggactgg agtttttccg tcgttgcgct atcataagcc
      481 cccgttcgcc atgtaaatcc ttcaataacc ttgtttcaac cgcaaaagat tgaacaacaa
      541 ttactagctt gagttctgcg tttcagtttc atccccgaaa gatcctggac gggcctgaaa
      601 gcgggttgcg aattcccggg taatggcggc aacaccgcaa atcctgcaaa gcttgagtta
      661 ttaattcagc cggagaccta tgagaaaact ttttacccga ctttccattc tgggcgcgct
      721 ctgcctcggg cagaccgccg cgtgggccag tgaagcggac ctgaagattc cggacctcca
      781 tgaagggcga ttcaacctgt tcggcggtct cacgggcttt cagatcctcc tttacggcgc
      841 gatagtgatc ctcggaacgg taggactttc cctctatctg ttcatgaggg tcaaggccct
      901 ccccgcccac aaatcgatgc tcgccgtggc ggagacgatt ttccagacct gcaaaacata
      961 cctcaagcag cagatcaagt tcctcacgat cctgttcgcg atcatcgcat gcacgatggg
     1021 ttattatttc gtcgggctca agcatgagcc gatgaccacc ctgggactga tccttctttt
     1081 ttcgatcgtt ggcatgggag gttccgccct ggtggcgtac tacgggattc gcatcaacac
     1141 ctacgcgaat gcccggacgg ccttcgcctc tttgcgcggg aaaccctggg atgttgtcaa
     1201 catccccatg tgttccggga tgtcggtcgg acttttcctg atctcccttg aactcgtaat
     1261 gatggtaatt atccttcttt tcgtacctcg cgatatcgtc ggatactgct ttttgggatt
     1321 tgccatcggc gaatcgctcg gcgcgagcgc gctgcgcatc gcgggaggca tcttcaccaa
     1381 gatcgccgat atcggttccg accttatgaa gatcgtcttc aaggtcaagg aggacgatcc
     1441 ccggaacccg ggcgtcattg ccgactgcac cggcgacaac gccggcgact cggtcggtcc
     1501 gaccgccgac ggtttcgaga cctacggcgt cacgggcgtc gcgctcatct ccttcatcac
     1561 gctcgcgatc aaggacccct cgcttcaggc gaagctcatc gtctggatct ttgcgatgcg
     1621 gttcctgatg gatttcatgt cgggggcctc ttacttcatc aaccagttca tctccgagcg
     1681 caagtacaag ggcctcaagg aattcaactt cgaggaaccc ctgacgcgtc tgatccagat
     1741 cgcgtcgacg ctttgcatca ctacctcgtt cgggatgtcg taccttctgg tcggcgatct
     1801 ccccgatccg acgctgtggt ggaagctcgc ttcgatcatt acgtgcggaa ccctggcggc
     1861 ctacctcatt ccggaattca ccaaggcgtt cacctcctcc cactccaggc acgttcagga
     1921 gatcgtgacg gcgtcgcggg aaggcggggc ctcgctcacg attctctcgg ggctcgtagc
     1981 gggttatttt tcggcgttct ggaaaggcat tctcatcgcg ggcctgatgt tcgtggccta
     2041 cctgatctcg ggaatggggc tccagaacat catgccgcac gcatcggtct ttgcgttcgg
     2101 tcttgtggcc tacggtttcc tctgcatggg tcccgtaaat atcgcggtgg actcgtacgg
     2161 accggtcacg gacaacgctc aatcgatctt cgagctggcc cagaccggat cgatccccgg
     2221 catcgaacag gagatcgaaa aagatttcgg tttcaagcct gatttcagga tcggcaaaca
     2281 ctacctcgaa gcgaacgact cggcgggaaa cacctttaaa gccaccgcca aacccgtgct
     2341 catcggaacg gcggtcgccg gcgcgaccac gatgatcttc tcgatcatcc tgctcctcca
     2401 ggagcaactt cactcaggcg ctatcgccgc ggcattaggg ggattggtcc ctgaaaatat
     2461 cggcgaaacc ctgttgaatg cgaagctgtc gctcacggcc gccccgatcc ttttgggcat
     2521 tctggccggc ggcgccgtga tcttctggtt ctgcggtgct tccatccagg ccgtgaccac
     2581 gggggcttat tccgcggtcg agtacatcaa gaaaaacatg aacctcgata aatcagaagc
     2641 cgaccgcaag gattcggtca cggtcgtccg gatctgcacc gagtacgccc agaaaggcat
     2701 gtggaacatc ttcctcgggc tcctgatgct gaccctcgcg ttcgcgctct tcgacccgta
     2761 tttcttcatc gcctacctca tcgccattgc caccttcggt ctgtacaagg ccatgttcat
     2821 ggcgaatgcc ggcggttcgt gggacaacgc gaagaaggtc gtcgaagtgg atctcggcga
     2881 gaagaacacg ccggtccacg ccgcgacagt catcggcgat accgtgggcg atcccttcaa
     2941 ggacacctcg tcggtctcgc tgaacccgat tatcaagttc tcgacgcttt tcggcatgct
     3001 ggccgtcgag atcgcgatca agatgaaccc ggcgacgacc cgcgtgagcg gagcggtcct
     3061 tttcgctctg ggtctcgtct ttgtctggcg ttcgttttac aagatgcgga t
//
LOCUS       DAOOBX010000080         3078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_35218_length_3078_cov_3.996030, whole genome shotgun sequence.
ACCESSION   DAOOBX010000080 DAOOBX010000000
VERSION     DAOOBX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35218_length_3078_cov_3.996030"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..>3078
                     /locus_tag="PLY30_01750"
     CDS             268..>3078
                     /locus_tag="PLY30_01750"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPW64857.1"
                     /translation="MSNKEDRKSSSVITNSSGKGIEIRRYFTRAGVNPLDEVKYTRRS
                     SVITNPDGSVVFRMENAEVPESWSQLATDIVVSKYFRKAGVPGTGSETSVRQLVHRVA
                     HSIRLAGERLGNYFRTGEDAQAFEDELLHLLVEQKCAFNSPVWFNCGLDLYHIKGSGG
                     NWYWDPETDSVRETTDAYAHPQCSACFIQSVEDDLMSIFDLVKNEARLFKYGSGTGTN
                     FSKIRGRMEKLSGGGTSSGLMSFLEVLDRGAGATKSGGTTRRAAKMVCLDMDHPEIVD
                     FINWKVREERKVAALIAAGYSSDFNGEAYKTVGGQNANNSVRISDEFMKSCMENGKWQ
                     TTFRTTGQVCDTYDTRHLMRQISFAAWACADPGVQFDTTINDWHTCAGTGKIRASNPC
                     SEFMFLDNTACNLSSINVMKYLNEDGTFDIEAFRHTIRVFTTAMEIIVDHASYPTKEI
                     AQNSHLYRPLGLGYANLGTLLMVSGIPYDSPKAFAICSAITAIMTGHAYKTSAELAAA
                     KGPFTEYARNESSMLRVIEKHRAAAYQIPSEHCWDDLLKAAQEDWDLALEFGKHHGYR
                     NAQVSVIAPTGTIGLLMDCDTTGIEPDFALVKFKKLAGGGHFKIINQSVPEALKRLGY
                     SAVEVQNIVEYIQGTARLDGTPWINRETLTEKGFTTQDLAKIEAVLPSVFDLGFAFTK
                     WTLGEETLKRLGFKPEDYNRPDFNPLETLGFSHSEIEEANNVICGMMTIEGAPHLKHE
                     HLPVFDCANKCGKYGKRYLEAMSHVRMMAAAQPFISGAISKTVNLPNEMTVEEIEDIY
                     IQAWKMGLKAVALYRDGSKLSQPLNTKSKESAGEKPETPKLERKRLPKKRIGMTVEAR
                     VGGQKVYLRTGEYADGELGEIFIDIHKEGAAFRSMMNCFAIAVSLGLQYGVPLEEFVN
                     VFTFTRFEPQGMVEHPNIKISTS"
BASE COUNT          710 a          957 c          833 g          578 t
ORIGIN      
        1 atcccacccg ctcgattcgg ttctttaata ttcttcggaa tttttttaca aaaatttttc
       61 agagcttcaa aaccgccgga acttcgctcc ggattccgga cggttcaaag acacggcacg
      121 gagcaacgct tgaactgaca cgatgaaaat cagaaacttt gtttcagtag aagttttcgc
      181 atcgaatact acatatagta tttctccttc cattaattca caaaatatag taaaggtttt
      241 aaaaagcctt taccggaggc gctcccaatg agtaataaag aagacagaaa aagctcgtcc
      301 gtgatcacta attcttccgg aaaaggcatc gaaatccgcc gttatttcac gcgcgcgggc
      361 gtcaatcccc tcgacgaggt caaatacacg cggcgcagct ccgtgatcac gaacccggac
      421 ggttcagtgg tcttcaggat ggagaacgcc gaagtcccgg aatcctggag tcagctcgcg
      481 accgatatcg tcgtctccaa atacttccgc aaggcaggcg tccccgggac gggaagcgaa
      541 acgagcgtcc gccagcttgt tcatcgcgtc gcccactcga tccggcttgc cggggagcgg
      601 ctcgggaatt atttccggac cggggaagac gcccaggcct tcgaggatga gctcctccac
      661 ctgctcgtgg aacagaaatg cgccttcaac tcccccgtct ggttcaactg cgggctcgac
      721 ctctaccata ttaaaggaag cggcggcaac tggtactggg accccgaaac ggattcggtg
      781 cgcgaaacga cggatgccta tgcccacccc cagtgttccg cctgtttcat tcagtccgtg
      841 gaagacgacc tgatgtcgat cttcgacctg gtcaagaacg aggcacgtct tttcaaatac
      901 ggctccggta cgggcaccaa tttctcgaag attcgcggac gcatggaaaa actctcgggc
      961 ggcggcacct cgtccggcct catgtctttc ctcgaagtgc tcgatcgcgg ggcaggcgcc
     1021 acgaagtccg gcggcacgac gcggcgcgcg gcaaagatgg tctgtctcga catggaccat
     1081 cccgagatcg tggacttcat caactggaaa gtccgcgagg aacgcaaggt cgcggcgctg
     1141 atcgcggccg gctattcctc cgacttcaac ggagaggcct acaagaccgt cggcggccag
     1201 aacgccaaca attccgtccg catctccgat gagttcatga aatcctgcat ggaaaacggc
     1261 aagtggcaga cgaccttccg aacgacaggc caggtctgcg atacctacga cacgcgccac
     1321 ctgatgcggc agatctcctt cgccgcatgg gcctgcgcgg acccgggcgt ccagtttgat
     1381 accacgatca atgactggca cacctgtgcc ggcaccggca agatccgggc ctcgaacccc
     1441 tgctcggagt tcatgttcct ggacaacacc gcctgcaacc tgtcctccat caacgtgatg
     1501 aagtacctga acgaggacgg gaccttcgat atcgaggcct tccgccacac aatccgggtc
     1561 ttcacgaccg ccatggaaat catcgtcgat cacgcatcct acccgacgaa agaaatcgcg
     1621 cagaacagcc atctctaccg tcctctcggg ctcggctacg cgaacctcgg cacgctgctc
     1681 atggtctcgg gcatccccta cgacagtccg aaggccttcg cgatctgctc ggccatcacg
     1741 gcgatcatga caggtcacgc ctacaagacc tcggcggaac tcgcggccgc caaaggaccg
     1801 ttcaccgaat acgccaggaa cgaatcctcc atgctgcgcg tcatcgaaaa gcaccgtgcc
     1861 gccgcctatc agatcccctc cgagcactgc tgggacgacc tgctcaaggc cgcgcaggag
     1921 gactgggacc tcgcgctcga gttcggcaag caccacggct accggaacgc ccaggtctcc
     1981 gtgatcgccc cgaccgggac catcggcctt ctgatggact gtgatacgac cggcatcgaa
     2041 ccggatttcg cgctcgtgaa gttcaagaag ctggccggcg gcgggcactt caagatcatc
     2101 aatcaatccg tacccgaggc cctcaagcgg ctcggataca gtgcggtcga ggtccagaat
     2161 atcgtcgagt acatccaggg tactgcgaga ctcgatggca ccccgtggat caaccgcgag
     2221 acgttgactg aaaagggttt cacgacccag gatctcgcga agatcgaggc ggtcctgccc
     2281 tcggtcttcg atctcggttt cgcgttcacg aaatggacgc tcggcgagga aaccctgaaa
     2341 cgtctcggct tcaagccgga agactataac cggcccgatt ttaatcccct cgaaaccctc
     2401 ggtttctctc attccgagat cgaagaggcc aacaacgtca tctgcggcat gatgacgatc
     2461 gagggcgcgc cgcacctcaa gcacgagcat ctccccgtct tcgactgcgc gaacaagtgc
     2521 ggcaagtacg gcaagcgcta tcttgaggcg atgtcgcatg tccgcatgat ggccgcggcg
     2581 cagcctttca tctcgggcgc gatctccaaa accgtgaacc tgccgaacga aatgaccgtc
     2641 gaggagatcg aggacatcta catccaggcg tggaagatgg gcctcaaggc cgtcgcgctc
     2701 taccgggacg gctccaaatt gtctcaacct ctcaatacga aatccaagga atccgcgggc
     2761 gagaaaccgg agacgccgaa actggaacgg aaacggctcc cgaaaaaacg gatcggcatg
     2821 accgtggagg ccagggtcgg cggtcagaag gtctacctgc gaaccggcga gtacgccgac
     2881 ggcgagctcg gcgaaatctt catcgatatc cacaaggaag gcgcggcgtt ccgctcaatg
     2941 atgaactgct tcgcgatcgc cgtttcgctc ggtctccagt acggcgttcc gctcgaggaa
     3001 ttcgtgaacg tgttcacctt cacgcgtttc gagccgcagg gcatggtgga gcatccgaac
     3061 atcaagatat ccacctca
//
LOCUS       DAOOBX010000081         3075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_35265_length_3075_cov_3.605960, whole genome shotgun sequence.
ACCESSION   DAOOBX010000081 DAOOBX010000000
VERSION     DAOOBX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35265_length_3075_cov_3.605960"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLY30_01755"
     CDS             complement(<1..511)
                     /locus_tag="PLY30_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW64858.1"
                     /translation="MQLKDKVALITGAGRGIGQSIAVAFAKEGAKLVLSDLKKEFLTE
                     TETLAKQAGSPAITLTEGNVAKGEEVNEIVKIALDTHDRIDILVNVAGITKDGLLAMM
                     SEQDWDDVLSINLKSAFLFTKACARPMMKQRSGAIVNIASIVGIAGNAGQANYSASKG
                     GMIALTRTTA"
     gene            complement(638..1576)
                     /gene="fabD"
                     /locus_tag="PLY30_01760"
     CDS             complement(638..1576)
                     /gene="fabD"
                     /locus_tag="PLY30_01760"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPW64859.1"
                     /translation="MQKIGFLFPGQGAQSVGMGKDLFEQSAAARAVYEKADTALGYSL
                     SKICFEGPEPELTRTLYAQAAIFVTSMAALAALHEKLGDLRPALTAGLSLGEFSALAA
                     AKAISFEDALELIRVRAEAMEAAAQQNPGTMASVMGLDIEGCKTTALEAGCEIANLNT
                     PEQTVLSGTKESIEKACKIAEAKGAKRAIPLKVGGAFHSSLMKPAQDRLEAALKNTEI
                     REPACVFIPNVTAQKVSEPAVIRDLLAKQLTSSVQWVGTMAAAKAEGIVSFIEIGPGK
                     VLKGLARKCQPEFQVSNFGAIADLSAIEALNLIRKS"
     gene            complement(1582..2568)
                     /locus_tag="PLY30_01765"
     CDS             complement(1582..2568)
                     /locus_tag="PLY30_01765"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPW64860.1"
                     /translation="MTAPYRAALTGLGAYLPEKVLTNFELEKMVETSDEWIRTRTGIR
                     ERHVAAPGQSSSDLALPAAREAIENAGLTVKDIDLIIVATISPDMLFPSTACYLQAKL
                     GATCGAFDMAAACSGFPYAIAIAEGFVKSGLYKNVLVVGAEAITKFINWKDRSTCVLF
                     GDGAGAAVVSRSRDDHGILASNLGADGRESEILQIPAGGSMNPPTEETVKSGLHYLRM
                     AGPELFKIAVKTMEQAVRNVLKHGNLEVKDIACLIPHQANNRILQAVSERLEIDPARV
                     FINVDRYGNMSSASTAVALYEAVKTGAIKRGDYVVLVAFGGGLTWAANLIKW"
     gene            complement(2591..>3075)
                     /locus_tag="PLY30_01770"
     CDS             complement(2591..>3075)
                     /locus_tag="PLY30_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HPW64861.1"
                     /translation="SKGTEVLKEAYKLIRDSSLNFIGNIEGRDFFTGKADCIICDGFV
                     GNVCLKMIESILETMISMISREIRKNPISSIGAWFLQPALANLRRDTNYEEAGGAPLL
                     GVNGNVIIAHGISSARAIRNGIRVASELVTTQANNQIVEAIAPYLHQIAAPRNSQAAA
                     "
BASE COUNT          564 a          865 c          929 g          717 t
ORIGIN      
        1 tcgcggtggt acgcgtcagg gcaatcatcc cgcccttgga ggccgagtag ttcgcctgcc
       61 cggcgttacc ggcgatgccg acgatcgaag cgatgttcac gatggcacct gaccgctgct
      121 tcatcatcgg cctggcgcac gctttcgtga atagaaacgc actcttcaga ttgattgaaa
      181 gaacatcgtc ccaatcctgc tcggacatca ttgcgagcaa cccatccttc gtgatgcccg
      241 cgacgttcac gagtatatcg atgcgatcat gagtgtcaag ggctatcttg acgatctcgt
      301 tcacttcttc gccctttgca acgtttcctt ccgtcagtgt gatggcaggg cttcccgcct
      361 gcttcgcgag cgtttccgtt tccgtcaaaa actctttttt gaggtcggaa aggacaagtt
      421 tcgcgccctc ttttgcgaac gcgacggcga tcgactgtcc gattccgcgg ccggctcctg
      481 tgatcagtgc gactttgtct tttagttgca tggttgttcc tccgttcgag acaggatagc
      541 ggtaaccaac ctgtcttttt tattcagttt tgttctttgt tacggttacg cattaaaaat
      601 gaaacttaaa taccgcccgt ttctgacggg gcaagtccta tgatttgcgg atcaggttga
      661 gcgcttcgat tgcggagagg tccgcgatgg cgccgaaatt gctgacctgg aactccggtt
      721 ggcatttcct ggccaagcct ttcaggactt tgccgggccc gatctcgata aaggatacga
      781 tcccctccgc cttggccgcg gccatagtcc cgacccattg gaccgagctg gtcagttgtt
      841 ttgccaggag atcgcggatt accgcaggtt cggagacctt ttgcgccgtg acattgggga
      901 taaagacgca agccggttcc cggatttccg tgttcttcag agcggcttcg agacggtcct
      961 gagccggttt cataagcgac gagtggaaag cgccgccgac cttgagaggg atcgcgcgct
     1021 tggcgccttt tgcttcggcg atcttgcagg ctttttcgat cgattccttc gtgccggaaa
     1081 ggaccgtttg ctccggcgtg ttgagattgg cgatctcgca gccggcctca agcgccgtgg
     1141 tcttgcagcc ttcgatgtcg aggcccatca cggaggccat cgtgccggga ttctgctggg
     1201 cggccgcttc catcgcttcg gcacggaccc tgatgagttc gagagcgtcc tcgaatgaaa
     1261 tcgctttcgc ggcggccagc gcggagaatt ctccgagact gaggcctgcc gtgagcgccg
     1321 gacggagatc cccgagtttc tcatggagag cggccagcgc ggccatactg gtcacgaaga
     1381 ttgcggcctg cgcgtaaagg gtccgggtca actcgggctc gggcccttca aaacatattt
     1441 tggagagcga ataaccgagg gcggtgtccg ctttttcgta gaccgcgcgc gcggcagcgc
     1501 tctgttcgaa aaggtcttta cccatcccga ccgattgcgc gccctgcccg ggaaataaaa
     1561 agccgatctt ttgcatctgg ttcaccattt gatgaggttt gcggcccacg tgagaccacc
     1621 gccgaaggcg acgaggacga catagtcccc tcttttgatc gcaccggtct tgaccgcttc
     1681 gtaaagcgca accgcggtgg aggcgctcga catgttgccg taacggtcga cgttgatgaa
     1741 gacgcgagcg ggatcgatct caagtctttc cgagacggcc tgcaggatgc ggttattggc
     1801 ctggtgcggg atgaggcacg caatgtcctt gacctcgaga ttcccgtgct tcaggacgtt
     1861 tcggaccgcc tgttccatcg ttttgaccgc gatcttgaaa agttccgggc ccgccatgcg
     1921 cagataatgg aggccgcttt tgacggtttc ttcggtcggg gggttcatcg atccgcccgc
     1981 gggaatctgg agaatctcgc tttcacgtcc gtctgcgccg aggttcgagg cgagaatccc
     2041 gtggtcgtcc cgggaacgcg aaacaaccgc cgcgcccgcg ccgtccccga agaggacgca
     2101 ggtagaacgg tctttccagt tgatgaactt ggtgatggct tccgccccga cgacgaggac
     2161 atttttgtag agtcccgatt tcacaaaacc ttctgcgatg gcgatcgcgt acgggaaacc
     2221 ggagcaggcg gccgccatat cgaaggcccc gcaggtcgcg ccgagcttgg cctgcaagta
     2281 acatgcggtc gacggaaaca gcatgtccgg cgagatcgtg gcgacgatga tgagatcgat
     2341 gtccttgacg gtcagaccgg cgttttcaat ggcctcgcgt gcggccggaa gggccaggtc
     2401 gctcgaggat tgtccgggtg ccgcgacgtg gcgttcgcgg atgcccgtgc gcgtgcggat
     2461 ccactcatcg gatgtctcga ccattttctc gagctcgaaa ttggtcagga ccttttcggg
     2521 gagataggcg cccaatcccg tgagcgcggc gcgataaggt gcggtcatag gtaaagaaat
     2581 ttctaagcga tcacgcggcg gcctgcgaat tacgcggcgc ggctatttga tggagataag
     2641 gggcgatggc ttccacgatc tgattattgg cctgggttgt taccagttcg ctggcgaccc
     2701 ggattccgtt gcggatcgcc cgggccgatg aaatcccgtg tgcgatgatg acgttcccgt
     2761 ttacgccgag gagcggagcc cctcccgcct cttcgtaatt ggtatccctg cggaggttgg
     2821 cgagcgccgg ctgcaggaac caagcgccga tcgacgagat cggattcttg cggatttccc
     2881 ggctgatcat ggagatcatc gtttcgagga tgctctcgat catcttgagg cagacgttcc
     2941 cgacgaagcc gtcgcagatg atgcagtctg cctttccggt gaaaaagtcg cggccttcaa
     3001 tgtttccgat gaagttgagc gacgaatcgc ggatcagctt gtaggcttct ttcaggacct
     3061 cggtcccttt ggatt
//
LOCUS       DAOOBX010000082         3069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_35346_length_3069_cov_3.178169, whole genome shotgun sequence.
ACCESSION   DAOOBX010000082 DAOOBX010000000
VERSION     DAOOBX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35346_length_3069_cov_3.178169"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="PLY30_01775"
     CDS             <1..1488
                     /locus_tag="PLY30_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPW64862.1"
                     /translation="VRSLRQVTGRDILIIDLSGDLATKLPQDMADIRSSRLDLASVKV
                     ASEADAAPSISKSRYGFDFLALVRNDALAIEDKKFVSLLTCLSYQYDYMVLCLPPRET
                     AVSVKALHYSDRVYLWAESRAEAFSFASTKVNALCHDHGFSRGEVRIILQHGERVSEE
                     DLPGVGSAQEIQLFSMLPSLQYQRYRYERMLSYLAKEWSERLVGLVLGSGAAYGLAHI
                     GVLRVLEQEKIPIDVIAGSSIGALMGGFWAAGLDSYEIEKIAKQLDRKSAFFKLLGFR
                     DMSAFHMGFFKGDQIVRFLKPYFGDKTFQDLKIPLRVTGADLLTSEEVVFDSGNLLNA
                     LRASISIPGFFRPFPHKGSFLLDGGIIDPLPVKTLSSMGVRKIIAVNVLPGPKDLIRR
                     NELRRKALHAELQRQKPLPRMILRTMDRAKRRYTGNIFNVLMNTIQFMEYEIARMQAA
                     EADVFIHAAIADGHWIEFFSPDKYITEGVSRTREQLAEIKRLLVE"
     gene            1522..2688
                     /locus_tag="PLY30_01780"
     CDS             1522..2688
                     /locus_tag="PLY30_01780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPW64863.1"
                     /translation="MKEIVIAGACRTAQGKLGGALRSFTNQELGSVLIRALLERSKVD
                     PDAVDEVIMGCVGQQSDAHNVARVISVMAGIPPRVPAYTVNRNCASGMQALVSAAQAI
                     LSGSAGLVIAGGVEVMSAVPFVNRDMRFGKKLRHSVMIDSLWEGLRDPLSGMMMGETA
                     ELLAREFSLTRFEQDRYAMESHRRACAAAAGRFKDEIAPVCLPSRDRRLFDTDEGPLP
                     STSIEVLSQLPSTFCEGGTVTAGNSCQISDGAAALIVASRERAVSLGLRVMAKVKAFG
                     FTGCDPKRMGMGPVAATRKVLDLAGMTTKDIGLFEINESFAAQYLAVEKELHLDRSLV
                     NVNGGAIALGHPVGASGTRIVVTLLHEMIRRGCPAGLASLCVGGGQGGAVLLEL"
     gene            2792..>3069
                     /locus_tag="PLY30_01785"
     CDS             2792..>3069
                     /locus_tag="PLY30_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64864.1"
                     /translation="MKLNVIIRVCALIGIVAVHFAATNSARSGTAPSNRKVQAITSPQ
                     SMISDQIAKFVGRKTGKELMEERSKQTAVPAKPASKGVSVPAARTSPKT"
BASE COUNT          597 a          826 c          957 g          689 t
ORIGIN      
        1 gtgaggtccc tgcgtcaggt caccggacgg gatattctca tcatcgacct ctcgggagat
       61 ctggcgacaa agcttcctca ggatatggcc gatattcgct cgagtcgtct cgaccttgca
      121 tcggtcaagg ttgccagcga agcggatgcc gcgccgtcca tttcgaaaag tcgttatgga
      181 tttgattttc tcgcccttgt ccggaatgac gcgctggcga tcgaggacaa gaagttcgtg
      241 tctctcctga cctgcctcag ttatcagtat gactatatgg tcctctgcct tcctccccgg
      301 gaaaccgccg tatcggtcaa agccctgcat tactccgacc gggtttacct ctgggctgag
      361 agccgggcgg aggcgttctc attcgcttcg acgaaggtca atgccctttg tcacgatcat
      421 ggtttcagca ggggggaggt ccggattatc cttcagcacg gtgagcgcgt ttcggaggaa
      481 gacctcccgg gcgtcggctc ggcgcaggaa atccagcttt tctcaatgct cccgtcactt
      541 cagtatcagc gctatcgcta tgagcggatg ttgagttatc tcgccaagga gtggtcggag
      601 aggcttgtgg ggcttgtcct cgggagcggg gcggcgtatg gcctggcgca tatcggcgtg
      661 cttcgcgtcc ttgagcagga aaaaatcccg atcgatgtga tcgcgggctc cagcatcggg
      721 gctttgatgg gcgggttttg ggccgcggga ctggattcgt atgagatcga aaagatcgcc
      781 aaacagctcg accgcaagag cgcgttcttc aaactgctcg gttttcgcga tatgtccgcg
      841 tttcatatgg ggttcttcaa gggggaccag atcgtccgtt tcctcaagcc gtattttggc
      901 gataagacat tccaggacct gaagattccg ctccgggtga ccggtgcgga tttgctgacg
      961 tcggaggaag tcgtctttga ttcgggcaat ttgttgaacg ccctccgcgc ctccatctcg
     1021 atcccgggtt ttttccgtcc gttcccgcat aaggggagtt ttctcctgga cggcggtatc
     1081 atcgatccgc ttccggtcaa gacactgtcg tccatggggg tccggaagat cattgcggtc
     1141 aatgtgctgc cggggccgaa ggacctgatc cggcgtaacg aactgcggcg aaaggccctg
     1201 catgcggagc ttcaacggca gaaaccgctg cctcgtatga tcctgaggac gatggatcgg
     1261 gccaagcgac gttacacggg caatattttc aatgtcctga tgaatacgat ccagttcatg
     1321 gagtatgaga tcgcgaggat gcaggccgct gaagcggatg tctttatcca cgcggccatt
     1381 gccgacggtc actggatcga gttcttttcc cctgataaat atattaccga gggcgtgagc
     1441 cggacccggg aacagctggc cgagattaaa cgccttctgg tggaataacc cgcgtttaat
     1501 cccggaagaa aggattaccc gatgaaagag atcgtgattg cgggcgcctg ccgcacggcg
     1561 caagggaagc tgggcggggc cctccgatcg ttcaccaacc aggagcttgg ttcggtcctt
     1621 atccgggcgc ttttggaacg aagcaaggtc gatccggatg cggtggacga agtaatcatg
     1681 ggttgtgtcg gtcagcagtc ggatgcccat aatgtcgcgc gggtcatctc ggtcatggcg
     1741 ggtattccgc cgcgcgtccc cgcgtacacg gtcaaccgga actgcgcctc cggaatgcag
     1801 gccctcgtaa gcgccgcaca ggcaatcctc tccggcagcg ccgggcttgt gatcgcgggc
     1861 ggcgtcgaag tgatgtccgc agttccgttc gtgaaccggg atatgcgctt tggaaagaaa
     1921 ctcaggcact cggtcatgat tgactccctt tgggaaggcc ttcgggatcc gctgtccggg
     1981 atgatgatgg gggaaacggc ggagctgctt gcccgtgaat tctcgctgac ccgcttcgaa
     2041 caggaccgtt atgccatgga atcccaccgc agggcctgtg ccgccgcggc gggacgtttt
     2101 aaggatgaga ttgcgccggt ctgcctgccg tcgcgcgata gacggctctt tgatacggac
     2161 gaggggccgc tgccatccac atcgatcgaa gttttaagcc agctgccgtc taccttttgt
     2221 gaggggggga ccgtcacggc cggcaattcc tgccagattt cggatggcgc cgcggcgctg
     2281 attgtggcgt cccgcgagcg ggccgtatcc ctcggactcc gtgtgatggc gaaggtcaag
     2341 gcgtttggtt tcacgggttg tgatccgaaa cgtatgggga tggggccagt cgcggctacg
     2401 cggaaagtcc tggatttggc gggtatgaca acgaaagata tcggcctttt tgagatcaat
     2461 gagtcgttcg ccgcccagta tcttgcggtt gaaaaggagc ttcatctcga tcgaagcctg
     2521 gtcaacgtta acggcggcgc tattgcattg ggacaccccg tgggcgcttc cggaacacgg
     2581 atcgtcgtga ccctgttgca tgaaatgatc cgccggggtt gccccgccgg tctcgcgtca
     2641 ctttgtgtcg gcggcgggca gggcggtgct gtgttgctcg agttgtaaag gcgcataaaa
     2701 acattaagaa aacgcgggat ttttcgatat aatcagaagc gtatcgcttc acggtccgta
     2761 tccacgcttg tcaaacagag tgaggtgttg tttgaaactg aatgtcatca tacgggtttg
     2821 cgcgctgatc gggatcgtgg cagtccattt tgcggcaacg aacagtgcca gatcagggac
     2881 cgctccctcg aatcggaaag tgcaggccat cacctcgccg caatccatga tcagcgacca
     2941 gatcgcgaaa ttcgtgggcc gaaagacggg taaagagctc atggaggagc gatcgaaaca
     3001 aacggctgtg ccggcgaagc cggcgtcgaa aggcgtttcc gttcccgctg cccggacgtc
     3061 cccgaaaac
//
LOCUS       DAOOBX010000083         3046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_35699_length_3046_cov_4.309261, whole genome shotgun sequence.
ACCESSION   DAOOBX010000083 DAOOBX010000000
VERSION     DAOOBX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35699_length_3046_cov_4.309261"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PLY30_01790"
     CDS             <1..1335
                     /locus_tag="PLY30_01790"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HPW64865.1"
                     /translation="RIDKTEKRRKPQAPYTTSKLQQESYTRIGFSSANTMRIAQRLYE
                     GIELGEEGSVGLITYMRTDSVNVAAAAQQEAIQFIKEKFGADYLPDAPPVYKSRKGAQ
                     EAHEAVRPTSVYRTPELVKAYLAEDELKLYELIWRKFVASQMVPAVDEVTSVAILAGD
                     KYWFKTSGTKNLFPGFTAAFGEIRTERDTAKNGEEEPEAVEVPDLRVGQKLKLHELAG
                     DQHFTKPPARFNDASLVRMLEEKGIGRPSTYAPTISTLLDRDYCEKKSGALIPTELGE
                     IVTDLLVQHFPKVLETEFTAFMEEEFDEIEEGKMEWKQVLRDFNGPFSALLEKAREEM
                     KSFKREPIPTDHKCGLCGKQLLIKFGRFGQFLACSGFPECKYTRSIPTGFYCPESGCG
                     GELVKRKAKGRRAFYGCSNYPKCTYTTASLPKKEDKEGENPSPAGAPEASPQ"
     gene            1455..2333
                     /gene="xerC"
                     /locus_tag="PLY30_01795"
     CDS             1455..2333
                     /gene="xerC"
                     /locus_tag="PLY30_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPW64866.1"
                     /translation="MEKEVDDFLQFLITEKGASIHTTKNYSIDLREFRKFLGEKPLKD
                     VTYIDIRAFLAFLKSRDYSKSSVARKLACLRSFFKYLARENVMTQNPAVGISTPRKEK
                     RLPDFLNPDEVTKLLDAPNKPTWEEKRDKSILEMLYSSGLRVSELVGLNHDDCDLFGG
                     VLRVRGKGKKERIVPVGQVALNCLKSYLDEKPPKDLAPAFKKPLFVSRLGGRLTDRSV
                     RRMILKYVKRTGLGKEVSPHMFRHSFATHMLDRGADLRSVQELLGHASLSTTQIYTHV
                     TTQRLKDAYTAAHPRA"
     gene            2425..>3046
                     /locus_tag="PLY30_01800"
     CDS             2425..>3046
                     /locus_tag="PLY30_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPW64867.1"
                     /translation="MLSSIFYNLAFAVFGIFYLPVFLVKIKLADDPDRLWRERWGRLP
                     DSWKVRTEGQRTVWLHAVSVGEVNAVERFVTELLEQDPRLDIVLTTVTPTGQKIAQKL
                     AGRRVRVGYFPFDLTFAVRRCFDTICPEAVLLAETEVWPNLLSEAKRRNIPVGIVNGR
                     LSEKSFGRYRAAGFFLKNIWAGIGFVLAQTEEDAGRFRALGVPREAV"
BASE COUNT          693 a          818 c          958 g          577 t
ORIGIN      
        1 agaatcgaca agaccgagaa acgccgcaag ccccaggcgc cctacacgac gagcaagctg
       61 cagcaggaat cgtacacccg catcggattc tcctccgcaa acacgatgcg catcgcccag
      121 cggctctatg aaggtattga gcttggcgag gaggggagcg tgggtctgat tacctacatg
      181 cgcacggatt ccgtgaatgt cgccgcggcc gcccagcagg aggccatcca gttcatcaag
      241 gaaaaattcg gggcggatta tctccccgat gcgccgccgg tctacaagtc ccgcaagggg
      301 gcccaggaag cgcacgaggc ggtgcgtccc acatccgtgt accgtacgcc ggagctcgtg
      361 aaggcgtatc tcgccgaaga tgaactgaaa ctctatgagt tgatctggcg gaaatttgtc
      421 gcatcgcaga tggtccccgc cgtggacgaa gtcacgtcgg ttgcgatcct tgcgggggac
      481 aaatactggt tcaagacgag cgggacaaag aacctgttcc cggggttcac ggccgcgttc
      541 ggcgagatca ggacagaaag ggataccgcg aaaaacgggg aagaagagcc cgaggcggtg
      601 gaagtgcccg acttgcgggt cggacagaag ttgaaacttc atgagctcgc gggtgatcag
      661 catttcacga aaccgccggc gcgtttcaac gacgcgagcc ttgtccgtat gctcgaggaa
      721 aaagggatcg gacggccgag cacctacgcg ccgacgatct cgaccctgct ggaccgcgat
      781 tattgcgaaa agaaaagcgg ggcgctgatc ccgacggagc ttggtgagat cgtgaccgac
      841 ctcctcgtgc agcatttccc gaaggtgctc gagacggaat ttacggcgtt catggaagag
      901 gaattcgacg agatcgaaga aggcaagatg gaatggaaac aggtcctgcg ggatttcaac
      961 ggaccgttca gtgccctgct tgagaaagcc agggaagaga tgaaaagttt caaaagggag
     1021 ccgatcccca cggaccataa atgcggtctg tgcgggaaac agctcctgat aaagttcggc
     1081 cggttcgggc agtttctcgc ctgttccggt tttccggaat gcaaatacac ccgttcgatt
     1141 ccgaccggat tctattgtcc cgagtcgggc tgcggcgggg aactggtgaa gcgcaaggcc
     1201 aaaggccgcc gcgctttcta cggctgctcg aattatccca agtgcaccta cacgacggcg
     1261 agcctcccca aaaaagagga caaggaaggg gagaaccctt cgccggccgg agcgcctgaa
     1321 gcttcacccc agtaaaggaa cgggaatgcg tcggcagtgc ggcgtgaacc cggatcgatc
     1381 cagaatgaga atcgcggagt gcggacagac cttgggtccg gcccgcgggt ccgtgacggg
     1441 gcaaggggca agggatggaa aaagaagtcg atgatttttt gcagttcctg attaccgaaa
     1501 aaggcgcgtc gatacacacg acgaagaatt attcgatcga cctccgcgag ttccggaagt
     1561 tcctcgggga gaaacctctg aaggatgtca cctacattga tatccgcgcc tttcttgctt
     1621 ttctgaaatc acgcgattac agcaaaagct ccgtcgcgag gaaattggcc tgtctccggt
     1681 cttttttcaa atatcttgcc cgcgagaacg tcatgaccca gaaccccgcc gtgggaatct
     1741 cgacccccag gaaggagaag cgccttcccg attttctgaa tcccgacgag gttacgaagc
     1801 tcctggatgc ccccaacaag ccgacgtggg aggagaaacg ggacaagtcg attctcgaga
     1861 tgctttatag ttcggggctt cgcgtgagtg agctggtcgg gctgaaccac gacgactgcg
     1921 atcttttcgg cggcgttctg cgggtccgcg gcaaggggaa aaaagagaga atcgtcccgg
     1981 tcgggcaggt tgcgctgaat tgtctcaaaa gctatctgga tgaaaaaccg ccgaaggatc
     2041 tcgcgcccgc gttcaagaaa ccgctttttg tgagccgcct cggcggacgg ctgacggacc
     2101 ggagcgtccg gcgcatgatc ctgaaatatg tgaaacggac gggactcgga aaagaagtgt
     2161 cgccccacat gttccgccat tcgttcgcga ctcacatgct ggaccgcggc gcggacctga
     2221 ggagcgtaca ggaactcctc ggccacgcga gtctctcaac gacgcagatc tatacgcatg
     2281 tgacgacgca gcgtttgaag gatgcctaca cggcggcgca cccgagggcg taaaaaacag
     2341 gggtttgtaa cgcgggacca tgggatcgat gaggaaccgg ttccggtttc tgtggcatga
     2401 ggcaagttaa caaagggttc agggatgtta agctcgattt tctacaatct ggcgttcgcg
     2461 gtcttcggga tcttttatct tcccgtcttt ctcgtgaaaa tcaagctcgc ggacgatccc
     2521 gatcggctct ggcgcgaacg gtgggggcgt ctcccggatt cctggaaggt ccgaacggaa
     2581 gggcaacgga cggtgtggct ccacgcggtg agcgtggggg aagtgaacgc cgtcgaaagg
     2641 ttcgtcacgg aactcctgga acaggacccg cgccttgata tcgttctgac aacggtaacc
     2701 ccgacggggc agaagatcgc gcagaagctc gcgggccgga gggtccgcgt cggatatttt
     2761 ccgttcgatc tgacgttcgc ggtgcggagg tgttttgaca cgatttgtcc ggaggccgtg
     2821 ctgcttgccg aaacggaggt ctggccgaac cttttgagcg aggcgaaacg gcggaatatc
     2881 cctgtcggga tcgtgaacgg gcgtctttcc gaaaaatcct tcggccgtta ccgagcggcc
     2941 ggattttttc tgaaaaatat ctgggccggg atcggttttg tcctggctca gacggaagaa
     3001 gatgccggcc ggtttcgggc gctcggcgtc ccccgggaag ccgtcc
//
LOCUS       DAOOBX010000084         3032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_35932_length_3032_cov_3.007726, whole genome shotgun sequence.
ACCESSION   DAOOBX010000084 DAOOBX010000000
VERSION     DAOOBX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35932_length_3032_cov_3.007726"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLY30_01805"
     CDS             complement(<1..588)
                     /locus_tag="PLY30_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPW64868.1"
                     /translation="MRNRRVVVTGMGVLTPIGNTLEEFSANLRNGVSGKEAESVSDVF
                     GCRFPPVFRVKAFNPHQYGTHILDPFIQYAVAAAESAVQDARFDMSAVDPYAVGLSIS
                     SSKGGVYTLDSFRDRFLKHPSAILGARIYSSAMPNSGNQWIARRMKIKGPAKCYVAAC
                     ATGVAAVTAGFRMVQEGHADYCLAGASDASIVPILL"
     gene            complement(601..1329)
                     /locus_tag="PLY30_01810"
     CDS             complement(601..1329)
                     /locus_tag="PLY30_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW64869.1"
                     /translation="MNLLLSRKKILVTGGTSELGSAFVRQAVARGAEVHFTWHESKAR
                     ALALEKTGARKHRVDLGSTESMDVFLKEFLAAHETLDVLVHNAAATADARLGSLSEAE
                     WDRVLAVGLKAPFYLTQKLLPLLLKVRPSKVFMIVSRAGLEGLPGAANYAAAKGGLIA
                     MTKTLARELGKKQVLVNAVNPGFMKSRMTENVPEEMFQAALERSPTRTISDPEEVAGF
                     LVYLSSGLVRGVTGQVFHFESRPL"
     gene            complement(1334..2314)
                     /gene="bioB"
                     /locus_tag="PLY30_01815"
     CDS             complement(1334..2314)
                     /gene="bioB"
                     /locus_tag="PLY30_01815"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HPW64870.1"
                     /translation="MKEKVLAGGEVTFDEALRLAKVSSPEEIDLILASAHELTLHFRS
                     SKPDLCALVNAKSYLCGEDCKFCSQSARYETGVSRYPLMSPEKILEAAQQCEAAGIKS
                     FCIVTSGETLNDREFEQVRETFRILSEETDLCLDGSLGNLTTGQIAELRKVGMRRFNH
                     NLQCSREFYPKIVSTHTYDERMETLDFLKTNDVEICSGGIFGLGESTEDRLRLAFELK
                     KHEPHCLPVNILNPRPGTPLEKQSPIDPVDALKMIAIYRFIHPRANIKLAGGKELNLG
                     AFYQAKALKGGANGLVVGGYLTTRGNPVAEDLKILKRAGYTVKSEPVTAA"
     gene            complement(2380..>3032)
                     /locus_tag="PLY30_01820"
     CDS             complement(2380..>3032)
                     /locus_tag="PLY30_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPW64871.1"
                     /translation="GIPAFVENDANAAVLGEKWLGLDRDVRHMVYMFSGVGCGILINE
                     EIYRGATGAAGELGITSMRATRDFTQKIATQLGRWEMDIGMVRHARERLDKGEPSLIK
                     DYVKGDLDKISFKDIVRAVKEKDALAIKVAEQAGQDLGRKIAFIVNLLNPEVVVIGGG
                     IEDCGAALLDAVKSTVKEWAVEEAATQVKVIPSAFGENAVALGVVGIVAREVFAQA"
BASE COUNT          608 a          913 c          867 g          644 t
ORIGIN      
        1 cagcaggatc ggaacaatag aggcgtccga ggctcccgcg agacaatagt cggcgtggcc
       61 ttcctgcacc atgcgaaacc ccgcggtcac tgccgcgaca ccggtcgcgc acgccgccac
      121 ataacatttc gccgggcctt tgatcttcat ccgccgtgcg atccactggt ttccggaatt
      181 cggcatcgcg ctcgaataga tgcgcgcgcc cagaatcgca cttgggtgct tcaggaaccg
      241 gtcccggaag ctatccagcg tgtaaacccc tcccttggaa gagctgattg aaagaccgac
      301 cgcgtagggg tcaaccgcgc tcatatcgaa tcgcgcgtcc tggacggcgg attcagccgc
      361 cgcaacagcg tactggataa aggggtccag gatatgcgtg ccgtattggt gggggttgaa
      421 agccttgacg cggaaaacgg gcggaaaccg gcatccgaaa acatccgaaa cggactccgc
      481 ctcttttccc gaaaccccgt tccgcaggtt cgcggagaat tcttcgagcg tgtttccgat
      541 gggcgtcaac acccccatcc ctgtcacgac aacccgtcgg tttctcatgg aaatgggcta
      601 ttatagcgga cgcgactcaa aatgaaacac ctgtcccgtg accccgcgca caagaccgga
      661 ggagagatag accagaaagc cggcaacctc ctccgggtcg gagattgtcc gggtcgggct
      721 cctctccaac gcggcctgaa acatttcttc cggaacgttt tcggtcatgc gcgacttcat
      781 gaaaccgggg ttcacggcgt ttacaagaac ctgttttttg cccagttcgc gcgcaagggt
      841 cttggtcatc gcgatcagac cgcccttggc ggccgcatag ttcgccgcac cgggaagtcc
      901 ctccagtccg gcgcggctta cgatcatgaa aaccttggac ggtctgacct tcaatagaag
      961 agggagcagt ttttgcgtga ggtaaaaagg cgccttgagc ccgactgcca gaacacggtc
     1021 ccattcggct tcgctgagac tcccgaggcg ggcatccgcg gtcgccgcgg cattgtggac
     1081 cagaacatcc agggtttcgt gcgcggccag gaactccttc aggaatacgt ccattgactc
     1141 tgtggacccg agatccacgc ggtgttttcg ggcgcccgtt ttttcaaggg cgagggctct
     1201 cgctttcgat tcatgccagg tgaaatggac ttcggcgcca cgcgcgacgg cctgccggac
     1261 aaaggcgctt ccgagttcgc tcgtaccgcc tgtgaccaga atctttttac gggacaggag
     1321 gaggttcaaa tgttcacgcg gccgtgacgg gctcgctttt gaccgtataa ccagcccgct
     1381 tcaggatctt gaggtcttcg gcaacgggat tcccccgtgt cgtcaaataa ccgcccacga
     1441 cgagcccgtt cgccccgccc ttcagggctt ttgcctgata aaaggccccg aggttgagct
     1501 ctttgccgcc cgcaagcttg atgttggcgc gcggatggat gaaacggtag atcgcgatca
     1561 ttttcagagc gtcaacagga tcgatcggcg attgcttttc gagcggtgtg ccgggccggg
     1621 ggttcaggat attgacggga aggcagtgcg gttcgtgttt tttcagctcg aacgcgagcc
     1681 ggagccggtc ttccgtgctt tccccaagtc cgaagatccc tcccgaacag atctcgacat
     1741 cgttcgtttt gaggaagtcc agggtttcca tgcgttcgtc ataggtatgt gtcgaaacga
     1801 tctttgggta gaactcgcgc gagcattgaa gattatgatt gaagcgccgc atgccgacct
     1861 tgcgaagttc cgcaatctgc ccggtcgtca ggttcccgag ggacccgtcc aggcaaagat
     1921 ccgtctcctc cgaaagtatc cggaacgttt cccggacctg ttcgaactcc cggtcgttca
     1981 gagtctcccc gctcgtcacg atgcaaaaac ttttgatacc ggcggcttcg cactgttggg
     2041 cggcctcgag gatcttttcg gggctcatca aggggtagcg gctcacgccg gtctcgtagc
     2101 gggcggattg cgagcagaat ttgcagtctt ctccgcaaag atagctcttg gcgttcacga
     2161 gcgcgcaaag atcaggcttc gaagatcgga aatgaagggt cagttcatgg gcggaagcga
     2221 gaatcagatc tatctcctcg ggagaggaga ccttcgcgag gcgaagggct tcgtcgaaag
     2281 taacctcgcc gccggcaagg actttttctt tcatctcccg gacgaaagga acgattgacg
     2341 gatcgatggg atcgcgaaac atcgggagcg cgccgttctt caggcctgcg cgaaaacttc
     2401 acgcgccacg ataccgacga ctccgagcgc tacggcattc tcgccgaacg cggacgggat
     2461 cactttgact tgtgtggccg cttcctcgac cgcccattcc ttgactgtgc tcttgacagc
     2521 gtcgaggagg gccgcgccgc agtcttctat gccgccgccg atcacgacaa cttcggggtt
     2581 gagaagattc acgatgaacg cgatcttcct tccgagatcc tgtcccgcct gctcagcgac
     2641 cttgatcgcc agcgcgtcct tttccttcac cgcgcgaaca atatccttaa aactgatctt
     2701 gtcgagatcg cctttaacgt aatccttgat cagggagggc tcgcctttat cgagacgctc
     2761 gcgcgcgtga cggaccatac cgatgtccat ctcccagcgt ccgagttgtg tcgcgatctt
     2821 ttgagtgaag tccctggtcg cgcgcatgct cgtgatccca agctctcccg ccgcgcccgt
     2881 cgcgccgcgg tagatctctt cattgatcag aatgccgcaa ccaacgcctg agaacatata
     2941 aaccatatgc cggacatcgc ggtcgagccc gagccacttt tcgccgagca cggccgcatt
     3001 cgcgtcattc tcgacgaacg cgggaatgcc ga
//
LOCUS       DAOOBX010000085         3025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_36027_length_3025_cov_3.481818, whole genome shotgun sequence.
ACCESSION   DAOOBX010000085 DAOOBX010000000
VERSION     DAOOBX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36027_length_3025_cov_3.481818"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /gene="tal"
                     /locus_tag="PLY30_01825"
     CDS             complement(<1..1015)
                     /gene="tal"
                     /locus_tag="PLY30_01825"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HPW64872.1"
                     /translation="METVMESMPSVELYQAGQGPWLDTISRRLLRSGQLKKWIEKDGL
                     LGVTSNPSIFQQAISQGDGYEQDIRKLFRKGATTLDVYDALTIADITETCDLFLPVFE
                     RTRGEHGFVSLEVLPCLAANTEATVREAKRLFDEVRRPNLMIKVPATPEGIVAVEELV
                     AEGINVNVTLIFSQANYIAVAQAYLAGLARLAGKGGTLAKVHSVASVFVSRIDTDIDK
                     QLDGKIATERDSAKRMTLEALKGKAAVANTKMVYQEFKRIFSSDAFRSLKDRGANIQK
                     VLWGSTSTKNPSYADLVYVENLVGKDSVNTLPQATWEALLDHGQIRPDAIEENIDEAR
                     GTVE"
     gene            complement(1160..2323)
                     /gene="hflX"
                     /locus_tag="PLY30_01830"
     CDS             complement(1160..2323)
                     /gene="hflX"
                     /locus_tag="PLY30_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPW64873.1"
                     /translation="MGPLMADRVFLVIVKTSRMSASDASVMLAEMRELTGSANGVVVG
                     ETRANIVDPSPSHYIRGGKIEELRVVTRETKANVVIFSVDLSPVQARNIESAIPWVRV
                     VDRTGLILDIFARRAVSKDGKLQVELAQLQYLLPRLVGQGVILSRLGGGIGTRGPGEQ
                     KLEVDRRKIRSRIEHIKEQIEKLSRHRALVRAGRRQKGLRQVAIVGYTNAGKSTLLNA
                     LTGGDAYVEDRLFATLDPKTRVLEARGGAPILFTDTVGFIRDLPHGLVKAFHATLEEA
                     TEADLILIVVDVSDELALEHKRVVDGVLKELGAEFRPKLVALNKMDLLNEQRRSRVLD
                     MFPGCHLVSAAQKKGLKELVSSIRGMLAIHEELNRQKREPGAGADAMPDMENR"
     gene            complement(2304..>3025)
                     /gene="miaA"
                     /locus_tag="PLY30_01835"
     CDS             complement(2304..>3025)
                     /gene="miaA"
                     /locus_tag="PLY30_01835"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPW64874.1"
                     /translation="ALKVLREITGRGHLPIVAGGTGFYVRALLEGLSSQPGANAHLRR
                     KLEREALLKGPEALYAKLLKADPVRAKAIGPHNLRRIIRALEIYRVSGRKPSEPLKKS
                     PGLGELGYRPVVIGLNRDRAALYDAINRRVDTMFRKGLVREVRRLAKQRLSDTARQGV
                     GYKELFPVIAAAGVNAGCRYRASSEVKDRIKQATRHFAKRQWTWFRREKGIRWVDWPE
                     GVPVAEMARRIAKDFFGASHG"
BASE COUNT          586 a          967 c          847 g          625 t
ORIGIN      
        1 gttcaacggt accgcgagcc tcatcgatat tctcttcgat cgcatcagga cggatctgcc
       61 cgtggtccag caaggcctcc caggtcgcct ggggaagcgt gttcacgctg tccttgccga
      121 cgaggttctc gacataaacc aggtccgcgt acgaaggatt ctttgtgctc gtactgcccc
      181 agagaacctt ctggatattg gccccgcgat ccttgagcga acgaaacgcg tccgaagaaa
      241 agattcgttt gaactcctgg tagaccattt tcgtgttggc gaccgcggct ttccctttca
      301 aggcctcgag cgtcatgcgc ttcgcgctat cgcgctccgt cgctatcttt ccgtcgagtt
      361 gtttgtcgat atcggtatcg atacggctca cgaaaacgct tgcgaccgaa tggacttttg
      421 cgagcgtccc gccttttccc gcaaggcgcg caagacccgc caggtacgct tgggcaacgg
      481 cgatgtaatt cgcctgagag aaaataagcg tgacattgac attgatgcct tcggcgacaa
      541 gctcctcgac cgccacgatc ccctcgggcg tcgcgggaac cttgatcatg aggttcgggc
      601 gcctgacttc gtcaaaaaga cgcttcgctt cgcggaccgt agcctcagtg ttggccgcga
      661 gacatggaag gacctcaaga ctgacgaatc cgtgctcacc cctggtacgc tcgaacaccg
      721 ggaggaaaag atcgcaagtc tccgtgatgt cagcgatcgt aagggcgtcg taaacatcga
      781 gggtcgtcgc tcccttcctg aacagcttgc ggatatcctg ctcataaccg tcgccctggg
      841 agatcgcctg ctggaagatc gaagggttcg atgtcacgcc gagaaggccg tccttttcga
      901 tccatttttt cagttggccc gaccgtaata acctgcggct gatggtatcc agccagggtc
      961 cctgacccgc ttgataaagt tcaacgctcg gcatcgattc catgacagtt tccacttgga
     1021 tttccttttt ttatttcatc gtcccggaac cgcgcggcgg gcgatccgga accgccgcaa
     1081 catccggaat gacgaggatt tcaggatcat cactagttaa tttaaggagg ggtaagacgg
     1141 gtgaagtata gcatgagcgc tagcggtttt ccatatccgg catcgcgtcg gcgccggcac
     1201 ccggctcccg cttctgacgg ttcaattcct catgaatggc cagcatcccg cgaatcgagc
     1261 tgacaagctc cttcaagccc ttcttttgcg cggccgaaac cagatggcat cccgggaaca
     1321 tgtcgaggac ccgggagcgt ctttgttcgt tcaggagatc cattttgttg agcgcgacaa
     1381 gcttcggccg gaactccgct ccgagctcct tcaggacacc gtcgaccact cgcttgtgtt
     1441 ccagggccag ctcgtccgaa acgtccacga cgatcaggat caggtcggct tccgtcgctt
     1501 cctcgagcgt cgcgtggaag gccttgacga gcccgtgagg aaggtcccgg atgaaaccaa
     1561 cggtgtcggt gaaaaggatc ggcgctccgc cacgcgcttc aagcacccgt gttttgggat
     1621 cgagcgtcgc gaaaagacgg tcctcgacat aagcgtcccc gccggtcagc gcgttcagga
     1681 gtgtggactt tccggcgttc gtatagccga cgatggcgac ctggcgcaga cctttctgac
     1741 ggcgacccgc gcgcacaagc gcgcgatgcc gggacagttt ttcgatctgt tccttgatgt
     1801 gctcaatgcg gctcctgatc ttgcgacggt cgacctcgag tttctgctcg cccggccccc
     1861 gcgtcccgat accgccgcca aggcgcgaca ggatcacgcc ctgtccgacc agacgcggca
     1921 ggagatactg gagctgcgcg agttcaacct ggagcttgcc gtccttcgaa accgcccgcc
     1981 gggcgaaaat atcgaggatt aaacccgtgc ggtcgaccac gcgcacccag gggatggcgc
     2041 tttcgatatt gcgggcctgc acgggcgaca aatcgacgct gaagatcacg acattcgcct
     2101 tcgtttcccg ggtcacgacg cggagctcct cgatcttccc gccgcggatg taatggctcg
     2161 gactcgggtc cacgatgttc gcgcgcgttt cgccgaccac aacgccgttc gcggatccgg
     2221 tcagctcccg catctccgcg agcatcaccg aggcgtccga cgcgctcatc cgggaggtct
     2281 tgacgatcac aaggaatacc cgatcagcca tgagaggccc caaaaaaatc cttcgcaatg
     2341 cggcgagcca tttcagcgac cggcacgcct tccggccaat cgacccagcg gatgcccttc
     2401 tcccgcctga accacgtcca ttgccgcttc gcaaaatgcc gcgtggcctg tttgatccga
     2461 tccttcacct cgctcgacgc ccggtatcga cacccggcgt tcacacccgc tgccgcaata
     2521 accgggaaaa gctccttata gccaacgcct tgccgcgcgg tgtcggaaag ccgttgcttc
     2581 gcgaggcgcc gcacttcacg gaccagcccc ttccggaaca tcgtgtccac gcgccggttg
     2641 atcgcgtcat aaagggccgc gcgatcccgg ttcaatccga tcacgacagg ccggtagccg
     2701 agctccccaa gtcccggact ctttttcagg ggttcggatg gtttcctgcc cgagacccgg
     2761 tagatctcaa gcgcccggat gatccgtcgc aggttatgcg gtccgatcgc cttcgcgcgg
     2821 acgggatccg ccttcaaaag cttcgcataa agggcttcag gcccttttaa aagggcctcc
     2881 cgctcaagct ttctccggag atgggcattc gcgcccggct gggacgaaag cccctctaat
     2941 aaagccctga cgtaaaaacc ggtccccccc gcgacgatgg gcagatgtcc ccgccccgtg
     3001 atctcgcgaa ggaccttgag ggcat
//
LOCUS       DAOOBX010000086         3020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_36104_length_3020_cov_3.239798, whole genome shotgun sequence.
ACCESSION   DAOOBX010000086 DAOOBX010000000
VERSION     DAOOBX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36104_length_3020_cov_3.239798"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PLY30_01840"
     CDS             <1..779
                     /locus_tag="PLY30_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPW64875.1"
                     /translation="KRKISVGDKMAGRHGNKGVIAQILPEEDMPFLQDGTPIDIILNP
                     LGVPSRMNVGQILETHLGWAAKALGLHVETPVFSGATEAEVKAMMKEANLPDDGKVTL
                     IDGRTGDPFDNKTTVGYIYMLKLAHLADDKIHARSIGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAFTLQELLTVKSDDVVGRTRVYEAIVKGEPALHAGTPESFNVLVK
                     ELQALGLDIAPERASGAKLPTLDAKSALESHDEAKEKLRV"
     gene            776..>3020
                     /gene="rpoC"
                     /locus_tag="PLY30_01845"
     CDS             776..>3020
                     /gene="rpoC"
                     /locus_tag="PLY30_01845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPW64876.1"
                     /translation="MNYNVNMFDSVAIRIAAPEVIRKWSKGEVRKPETINYRTLKPEK
                     DGLFCEKIFGPTKDYECACGKYKRIKHKGIVCDRCSVEVTQARVRRERMGHIDLVTPV
                     SHIWFFKTMPSRIGNLLDLSVRALEKVLYYEEYIVIDPKQTPLKARQLLTEEEYHKAQ
                     EQYGLDAFDAMMGAEAIQKLLSGIDLDKLTQKYQRQVKASKSKQSRARSVKTLKIVEA
                     FRKSGNRPEWMIMKVIPVIPPDLRPLVALDGGRFATSDLNDLYRRVINRNNRLRKLLD
                     LKAPDVIIRNEKRMLQEAVDALFDNGRHGRPVLGAGNRPLKSLSDMLKGKQGRFRQNL
                     LGKRVDYSGRSVIVIGPDLKLHQCGLPKKMALELFEPFIIRKLKERGHVHTIRSAKKM
                     VERVQPEVWDILEEVIHEHPVMLNRAPTLHRLGIQAFEPMLIEGNAIRIHPLVCTAFN
                     ADFDGDQMAVHVPLSMEALMEARILMLSANNIFSPASGNPIMTPTQDIVLGIYYLTKM
                     REGAKGEGLVFSNFQEAFLAYQDGQIHKHAKCKVRLVDGKIVDTTMGRIILNETLPEG
                     FRYVNEVVSKGKLSKIIADCYAQFGHAVVVDLLDRLKSLGFEAATSGGISIGIDDIRI
                     PEKKEDILVEARKEVKSIESQYKQGIITDGERKNKIIDIWTHATDRVSDIMFEELDQF
                     NPVFMMADSGARGSKQQIGQLAGMRGLMAKPSGEIIESPITANFREGLTVLDYFISTH
                     GARKGLAD"
BASE COUNT          670 a          859 c          878 g          613 t
ORIGIN      
        1 cgaaacgcaa gatttcggtc ggcgacaaaa tggccgggcg tcacgggaac aagggtgtca
       61 tcgcgcaaat ccttccggaa gaagatatgc cgtttctcca ggacgggact ccgatcgata
      121 tcatcctgaa tccgctcggt gttccgtcgc gtatgaacgt cggtcagatt ctcgaaaccc
      181 atctcggatg ggcggccaag gctcttggtc ttcatgtgga aacgcccgtc ttcagcggcg
      241 cgaccgaagc cgaggtcaag gcgatgatga aagaagccaa tcttccggat gatggcaagg
      301 tgacgctgat cgatggtcgt accggcgatc cgttcgataa caagacgacc gttggctata
      361 tctacatgct gaagctcgcg cacctcgcgg atgacaagat tcacgcgcgt tcgatcggtc
      421 cgtactcgct cgttacgcag cagccgctcg gcggcaaggc gcagttcggc ggccagcgtt
      481 tcggtgaaat ggaagtgtgg gcccttgagg cctacggcgc ggctttcacg ctccaggaac
      541 tcctgaccgt gaaatccgac gatgttgtcg gtcgcacgcg cgtctacgaa gccatcgtga
      601 aaggtgaacc cgcgcttcac gcgggaacgc ccgaatcctt taacgttctc gtgaaggaac
      661 tgcaggcgct cggtcttgac atcgctccgg aacgtgccag cggtgccaag cttccgacgc
      721 tcgatgccaa atccgcgtta gagtcccatg atgaggcgaa ggagaagctc agggtatgaa
      781 ctataatgtc aatatgttcg attcagtcgc aatccgcatc gccgctcccg aagtcatccg
      841 caaatggtcg aagggggagg tcaggaagcc ggaaacgatc aattaccgga ccctgaaacc
      901 ggaaaaagac ggacttttct gcgaaaagat ctttggtccc acgaaggact atgagtgcgc
      961 ttgcggcaag tacaagcgaa tcaagcacaa agggatcgtt tgcgaccgtt gtagtgttga
     1021 ggtcacgcag gcgcgtgttc gtcgcgagcg catgggacat atcgatctcg tgacgcccgt
     1081 ctctcatatc tggtttttca aaaccatgcc gtcccgtatc gggaacctcc tcgacctgag
     1141 cgtccgtgct cttgagaagg tcctctatta tgaagagtac atcgtcatcg atccgaagca
     1201 gacgccgctc aaggcgcgtc agctcctgac cgaggaagag taccacaagg cccaggagca
     1261 gtacgggctc gacgcgttcg acgccatgat gggcgccgaa gccatccaga agctcctgtc
     1321 cgggatcgac ctcgataagc ttacgcaaaa atatcagcgc caggtcaagg cctccaagtc
     1381 caagcagtcc cgtgcgcggt ccgtcaagac cctcaagatc gtcgaggcat tccgcaagtc
     1441 ggggaaccgc cccgagtgga tgattatgaa agtcattccg gtcattccgc cggatcttcg
     1501 gcctcttgtt gccctggacg gtggccgttt cgcgacgagc gatctgaacg acctttaccg
     1561 ccgcgttatc aatcgcaaca accgtctccg caagcttctg gatctcaagg ctccggatgt
     1621 catcatccgt aatgagaagc gcatgcttca ggaagccgtg gatgcccttt ttgacaacgg
     1681 ccgtcatggt cggccggtgc tcggcgccgg caaccgcccg ctcaagtccc tgagcgacat
     1741 gctcaagggc aagcagggcc gtttccgcca gaaccttctc gggaaacgcg tggattattc
     1801 gggacgttcc gtcattgtca tcggtcccga tctcaagctc caccagtgcg gacttccgaa
     1861 gaagatggcc ctcgagcttt tcgaaccgtt catcattcgc aagctcaagg agcgcgggca
     1921 tgtgcacacg atccgttccg ccaagaagat ggtcgagcgc gtgcagcccg aagtctggga
     1981 tattttggaa gaggtcattc atgagcatcc ggtcatgctc aaccgcgccc cgacgcttca
     2041 ccgcctcggt atccaggcgt tcgagccgat gctgatcgag ggtaacgcga tccgcatcca
     2101 cccgctcgtt tgtacggcgt tcaatgccga cttcgacggc gaccagatgg ccgttcacgt
     2161 gccgctttcg atggaagcgc tcatggaagc gcgcatcctc atgctttcgg caaacaacat
     2221 cttctctccc gcgagcggca acccgatcat gaccccgacg caggatatcg tgctcggaat
     2281 ctactacctc accaagatgc gtgaaggcgc gaagggcgag gggctcgtgt tcagcaattt
     2341 ccaggaagcg ttcctcgctt atcaggatgg gcagattcac aagcacgcga agtgcaaggt
     2401 gcggcttgtg gacggcaaga tcgttgacac gacgatgggc cggatcatcc tgaacgagac
     2461 cctcccggag ggcttccggt atgtcaatga ggtggtcagc aaggggaagc tctcgaagat
     2521 catcgcggat tgttacgcgc agttcggtca cgcggtcgtg gttgaccttc tcgatcggtt
     2581 gaagtccctc ggatttgaag cggccacaag cggtggcatc tcgatcggga tcgatgacat
     2641 ccggatcccc gagaagaaag aggatatcct tgttgaagcg cgcaaggaag tgaagtcgat
     2701 cgagagccag tacaagcagg gtatcatcac ggatggtgag cgcaagaaca agatcatcga
     2761 tatctggacc cacgcgacag accgcgtgtc ggatatcatg tttgaagagc tcgatcagtt
     2821 taacccggtc tttatgatgg ccgattccgg cgcccgcgga tccaaacagc agatcggtca
     2881 gcttgccggt atgcgcggtc ttatggcgaa gccgtcgggc gagatcattg aaagcccgat
     2941 cacggcgaat ttccgtgaag ggttgaccgt gctcgactac ttcatctcga cccacggcgc
     3001 ccgtaagggt ctggctgata
//
LOCUS       DAOOBX010000087         3015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_36199_length_3015_cov_3.114865, whole genome shotgun sequence.
ACCESSION   DAOOBX010000087 DAOOBX010000000
VERSION     DAOOBX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36199_length_3015_cov_3.114865"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3004
                     /locus_tag="PLY30_01850"
     CDS             <1..3004
                     /locus_tag="PLY30_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64877.1"
                     /translation="LLRAELEGLRAAGITPNLDTMKVQVSAGIDLAKGNLYKIPMGFG
                     KTLTLRVAGYLLTNNKDKTKNYTTAQGKGTLVATVNDDLAFRDALSAGAIFSKFGTRV
                     GVIKDEMVKRTVDGKETEVPDKVGYFYDAETGEMKKTAADEVYRDADIIYGTIDKFVH
                     RSLDEELALDKDHVIFSGRKYFVLFDEADEPLTYQSTNPFIISGGEKADAFLARSQHI
                     RARMLALEILAAEEQSAGKALVNRSRAASKKITLTDAGLERLGEVLKGQGSELLAGTG
                     LGADTTLWKQLVELALSVELAYNAPGEKKQYEIVTDKNGVKEIVIIGENGKLQPGSRW
                     SNGIHTAVEVKHMAEGVEVKLDQGTRNMTKLGKFLMKSGLVENFGGASGTMDLEAMKA
                     NYNKTPVEPDVGPGSRTETLQIYKTEADKWAAFRDDVIAQHAKDIPIIIQVADGQVDR
                     VAQDLLDHGLPKDTVIRKFDAGSDTDLAAIEKHGGEAGAITIVTRRGGRGVDIKLTEV
                     FKKLNDEMAKTGRVFTGYSTFIDPIEANDIQFRGRVGRRGEPGVFKAFWSLEDSIFGE
                     YLETPGVKAVLAALRGEFNAAGDPVIETAKPGEKGPTDIADLIAAMRRSIMDSRIKQA
                     KSEEQYDDEIDRRKAEFLKLREAVLNENYSDVALNDALPGIAAAAAGIAPADKVTFRR
                     GILEVLDRGLSRYLDPVGNARADLVRESQEDWIGAAAQQDPYVKFRGISASAHANAMD
                     FVRLLASQKTMGRISAERAQPFLNINWTTVRTKVVSYGVIGSVIAGMVWFISAATKYI
                     GIVQALASTGATMAEVVAKFKLLSMLASAFTALPAALWVVAAIVLVAVGFTLTQLYVK
                     RFSQSYKGQQKLSLAFKGLATRQEAAEALLSFPVQLLTMTGAMMPATSLFALIAALAF
                     PVTAPVLIPAAVVMAAIGVLSSVALIAFNYKRHIKDTKLDQASNFSRGLRVVFFGLTA
                     ITGGGMLLMLVPSKVPGLLAAVAIGRTG"
BASE COUNT          657 a          815 c          915 g          628 t
ORIGIN      
        1 gctccttcgc gcagaactgg aaggcctccg cgcggccggc atcacgccaa accttgacac
       61 catgaaggtc caggtgtccg cgggtatcga tcttgccaaa ggcaatcttt acaagatccc
      121 gatgggtttc ggtaagacgc tgaccctgcg cgtggccgga tatctgctta ccaataacaa
      181 agacaagaca aagaattaca ccacggcgca ggggaaaggg actcttgtcg ccaccgtcaa
      241 cgatgatctc gcgttccgcg atgccctgtc ggcgggagcc atcttttcga agttcgggac
      301 ccgcgtcggc gttatcaagg atgaaatggt caaacggacc gtggacggca aggaaactga
      361 agtgccggac aaagtcggct atttttatga cgccgaaacg ggtgagatga aaaaaaccgc
      421 ggccgatgaa gtctatcgtg atgccgacat catttacggg acgatcgaca agttcgtgca
      481 ccgctcgctg gatgaagaac tcgcgctcga caaggaccat gtgatctttt ccggacgaaa
      541 atactttgtt ctttttgacg aagctgacga gcctctgacc tatcagtcga cgaatccgtt
      601 cattatctcg ggtggtgaga aagctgacgc gtttcttgcc cgctctcagc atattcgtgc
      661 gcgaatgctc gcgctggaga ttcttgccgc ggaagaacaa tcggccggaa aggccctcgt
      721 gaaccggtcc agggcggctt cgaagaagat tacgttgacc gatgccggtc tggagcggct
      781 tggcgaggtc ctgaaggggc agggatcaga gcttctggcc ggaacggggc ttggcgcgga
      841 tacgacgctc tggaagcagc tcgttgaact ggccctgtca gttgagctcg cgtataacgc
      901 tcccggggag aaaaaacagt atgagatcgt aacggataaa aacggcgtca aggagatcgt
      961 cattatcggg gaaaacggca agcttcagcc gggatcgcgc tggtcgaacg ggattcacac
     1021 tgctgttgag gtcaagcata tggccgaagg cgtagaggtg aagctggatc aggggacccg
     1081 gaacatgacg aagctcggta agttcctcat gaaaagcggg ctggtcgaaa atttcggagg
     1141 cgccagcgga acgatggacc ttgaggccat gaaagctaac tataacaaga cgccggtgga
     1201 gcctgatgtc gggccgggca gccgcacgga aacacttcag atctataaaa cggaagcgga
     1261 caagtgggcc gcgttccgcg acgatgtgat cgctcagcac gccaaagaca tccctatcat
     1321 tatccaggtt gcggatggtc aggtcgaccg ggtggcccag gatcttctcg atcacgggct
     1381 tcccaaagat acggtcatcc gcaagtttga cgccggttcg gatacggacc ttgccgccat
     1441 tgaaaaacac ggcggcgaag cgggtgcgat cacgatcgtg acccggcgcg gcggccgcgg
     1501 tgtcgatatc aagctgacgg aggtctttaa gaaactgaac gatgaaatgg cgaagaccgg
     1561 aagggtgttt accggttatt cgaccttcat cgatccgatc gaagctaatg acattcagtt
     1621 ccgaggtcgt gtcggacgcc gcggggaacc gggcgttttc aaggctttct ggtcgctcga
     1681 agattccatt ttcggggagt acctcgaaac tccgggagtg aaagcggttt tagcggccct
     1741 tcgcggggag ttcaatgccg ccggtgatcc ggtcatcgaa accgccaagc ccggagaaaa
     1801 agggccgacg gatattgccg atctcattgc cgcgatgcgc cgctcgatca tggattccag
     1861 gatcaaacag gccaagtcgg aagaacagta tgatgatgag atcgaccgcc gcaaggcgga
     1921 gttcctcaaa ctccgcgagg cggtactcaa cgaaaattac tcggatgtgg cgcttaacga
     1981 tgcgcttccc ggcattgccg cggcagccgc gggaatcgcg ccggccgata aagtgacttt
     2041 ccgccggggt atcctcgaag tgctggaccg cgggctgagc cgttatctgg atcccgtcgg
     2101 caacgcgcgc gccgaccttg ttcgtgagtc gcaggaagac tggattggcg ctgccgccca
     2161 gcaggacccc tacgtgaaat tccgcggcat ctccgcgtcc gcgcacgcga acgcgatgga
     2221 ttttgttcgg ttgctcgcgt cgcagaagac gatggggaga atcagcgccg agagggcgca
     2281 gccgttcctc aatattaact ggaccacggt ccgcaccaag gtcgtcagtt acggagtgat
     2341 cggctccgtc attgcgggga tggtttggtt cataagcgcc gccacaaagt atatcggtat
     2401 tgttcaggct cttgcatcta cgggcgcgac gatggccgag gttgtcgcga aatttaaact
     2461 tctcagcatg ctcgcgtccg cgttcaccgc gctccccgcg gccttatggg tggtagccgc
     2521 aatcgttctt gtcgcggtcg ggtttacgct gacccagctt tacgtcaaac gtttctcgca
     2581 aagctataaa ggtcagcaga aattatcact cgcattcaag gggcttgcga ccaggcagga
     2641 ggccgccgaa gccctgcttt cattccctgt gcagcttttg acgatgacgg gggccatgat
     2701 gccggcgacg tcgctttttg cgcttatcgc ggctcttgcc ttccccgtga cggcgcctgt
     2761 tctgatccct gccgcggttg tgatggccgc gatcggcgtt ctttcctccg ttgccctcat
     2821 tgcgttcaac tacaaaaggc acatcaaaga cacaaaactc gatcaggcaa gcaatttttc
     2881 ccgggggctt cgcgtggttt tcttcggtct cacggcgatt accggtggtg gcatgctctt
     2941 gatgctggtc ccgtccaagg tgccggggct gcttgcggcg gttgccatcg gtcgtacggg
     3001 ttagcgcctt aacaa
//
LOCUS       DAOOBX010000088         2974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_36838_length_2974_cov_2.793080, whole genome shotgun sequence.
ACCESSION   DAOOBX010000088 DAOOBX010000000
VERSION     DAOOBX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36838_length_2974_cov_2.793080"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLY30_01855"
     CDS             <1..855
                     /locus_tag="PLY30_01855"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPW64878.1"
                     /translation="GDSYVLASETCAFDLIEAKFIREVEPGEVVVIDRKGVRSHFPHK
                     GMEGMGHQISQCIFEHVYFARPDSRIFHDNVGLVRERLGHQLAKEHPAKADIVIPVPD
                     SGTFAALGFSRESGIPLSFGFIRNHYIGRTFISPTEAGRSIKVKIKLNPIREMVEGKR
                     VVVVDDSIVRGNTAKNRVKLLRGAGAKEIHMRISCPPHVSPCYYGIDFPSKKELIAAN
                     QSASMIRKFLEVDSLGYLSLEGMLAATTQPPSHFCTACFSGKYPTPVFDEQDKLKPEN
                     KKVKCRGA"
     gene            845..1870
                     /gene="purM"
                     /locus_tag="PLY30_01860"
     CDS             845..1870
                     /gene="purM"
                     /locus_tag="PLY30_01860"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPW64879.1"
                     /translation="MAHKRWTYERAGVPHLKGDAAYNRKIAGLIRSTHVSGVMGNTLG
                     FASLFDLKKSGIRDPLLVASTDGVGTKLELARLIGKHDTIGIDLVAMCVNDLICCGAK
                     PLFFLDYYATGKFEPRTVGEVLKGVTDGCRQAGCALVGGETAIMPGFYGDRRAGQSAQ
                     YDIAGFSVGAVDRKKIVSGAAIREGDIVLGIQSSGFHSNGYSLVRRVFSEKELKGMIG
                     RELLTPTIIYVKPVLKVLEKVRLKGIVNITGGAFYDNLPRVMPKGLTALIDSHAWEIP
                     FIFRRAQKAGNISDFDMFKTFNMGIGMTLILGTRDVTKAQALLKTFRLKSWPIGKIVK
                     GHKVEVV"
     gene            1934..>2974
                     /gene="purD"
                     /locus_tag="PLY30_01865"
     CDS             1934..>2974
                     /gene="purD"
                     /locus_tag="PLY30_01865"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPW64880.1"
                     /translation="MMPENKRPEGGADKLKVLLVGGGGREHALAWKIAQSPKLAKLYC
                     APGNAGIAKHAECVNVSSDDLDGLLKFAVDNKIDLTVVGPELPLVAGIYDKFHARGLK
                     VFGPSAKMAMLEGSKSFSKDVMSRFGVPTAAYEVFTHAKEAMHYAVETEMPVVIKADG
                     LAAGKGVVMCDTSEQAVAALSGIMEKKVFGAAGDKVVIEQKLEGEELSILVLTDGETI
                     LPLASARDHKRAYEHDRGPNTGGMGAYSPSAQFDEASIGTIIDKTVRPIIDGLRKEGT
                     PYRGVLYAGLMMTKDGPYVLEYNCRFGDPETQVVLPMLQSDFGEIIAAAVANRLDEIR
                     PRWYNGSGVCVVL"
BASE COUNT          622 a          827 c          955 g          570 t
ORIGIN      
        1 ggggacagtt acgtactggc ctccgaaacc tgcgcgttcg acctgatcga agcgaagttc
       61 atccgtgagg tcgaaccggg cgaagtggtc gtgatcgaca ggaagggggt tcgcagccat
      121 ttcccgcaca aggggatgga gggcatgggc caccagattt cgcagtgcat ctttgagcat
      181 gtctatttcg cgcgtcccga ctcccggatc ttccacgaca atgtcggtct ggtccgcgaa
      241 cggttgggcc accagctcgc gaaagaacat cccgcgaaag ccgacatcgt gatcccggtg
      301 cccgattccg gcaccttcgc ggcgctgggg ttttcgaggg aatccgggat tccgctttcg
      361 ttcgggttca tccggaacca ttacatcgga cggaccttca tcagtcccac ggaggccggc
      421 cgttcgatca aggtcaagat caagctgaac ccgatccggg aaatggtgga gggaaagcgg
      481 gtcgtcgtgg tggacgactc gatcgttcgc ggcaacacgg cgaagaaccg cgtcaaactc
      541 ctgcgcggcg ccggggcgaa agagattcat atgaggatca gctgccctcc gcatgtttcg
      601 ccgtgctatt acgggatcga tttcccgtcg aagaaggaac tgatcgcggc gaatcaatcc
      661 gcgtccatga tcaggaagtt ccttgaggtt gacagcctcg gctacctgag cctcgagggc
      721 atgctcgcgg cgaccacaca gccgccctcc catttctgca cggcctgttt ttcggggaag
      781 tacccgaccc ccgtttttga cgagcaggac aaactgaaac ccgaaaacaa aaaggtgaaa
      841 tgccgtggcg cataaacgat ggacttatga aagagccggt gtgccgcacc tgaagggtga
      901 cgcggcctac aatcgaaaga tcgccggtct gatccgttcc acgcacgtga gcggtgtgat
      961 ggggaacacg ctcggtttcg cgtccctttt tgacctgaag aaatccggga tcagggatcc
     1021 gcttctcgtg gcgtcgaccg acggtgtcgg cacgaagctt gagcttgcgc ggctgatcgg
     1081 gaaacacgac acgatcggga tcgaccttgt cgcgatgtgc gtcaacgacc tcatctgctg
     1141 cggcgcgaaa ccgctttttt tcctcgatta ttacgcgacc gggaagttcg agccgcgcac
     1201 cgtcggcgag gtccttaaag gcgttacgga cggatgccgg caggcgggat gcgccctcgt
     1261 gggcggcgaa acggcgatca tgcccggttt ctacggggac cggcgcgcgg gccagtcggc
     1321 gcagtacgac atcgccgggt tctccgtcgg cgcggttgac cgcaagaaaa ttgtttcagg
     1381 cgccgcgatc cgcgaaggag acatcgtgct cggaatccag tcatcggggt tccattcgaa
     1441 cggttattcg ctcgttcggc gcgtcttttc cgaaaaggag ttgaaaggaa tgatcggccg
     1501 cgaactcctg acgcccacga tcatttatgt gaagccggtc ctgaaggtcc tcgagaaggt
     1561 gcgtctcaag gggatcgtca atatcacggg cggcgctttc tacgacaacc tgccgcgggt
     1621 gatgccgaag gggctcacgg ccctgatcga ttcccatgcc tgggagatac cgtttatttt
     1681 ccgcagggcg cagaaggccg gaaatatttc cgactttgat atgttcaaga cgttcaatat
     1741 ggggatcggg atgaccctga tcctcgggac gcgggacgtg acgaaggccc aggcgcttct
     1801 caaaacgttc aggctgaagt catggccgat cggaaagatc gtcaaaggcc ataaagtaga
     1861 agtggtttga atttccctga atgatccaat gccgcggccg tgtgagctgg gccgcgcgag
     1921 cgagagaccg gacatgatgc cagaaaacaa aagaccggaa ggcggtgcgg ataagctgaa
     1981 agtgctgctg gtgggtggcg gcggccgcga gcacgcgctt gcgtggaaga tcgcgcagag
     2041 cccgaaactg gcgaagctct actgcgcgcc cgggaacgcg gggatcgcga agcacgccga
     2101 atgcgtcaat gtctcctccg atgatctgga cgggctcctg aaattcgcgg ttgataataa
     2161 aatcgatctg acggtcgtgg gtcccgagtt gccgctcgtg gcggggattt atgacaaatt
     2221 ccacgccagg ggcctgaaag tcttcggacc ttccgcgaag atggcgatgc tcgagggcag
     2281 caaatcgttc tcgaaggatg tcatgagccg cttcggggtc ccgaccgcgg cttatgaggt
     2341 gttcacgcac gccaaagagg cgatgcacta cgcggtcgag accgaaatgc cggtcgtaat
     2401 caaggcggac ggactggcgg cgggcaaagg tgttgtgatg tgcgacactt ccgagcaggc
     2461 cgtggcggcg ctttccggga tcatggagaa aaaggtcttc ggggccgcgg gtgacaaggt
     2521 cgtgatcgaa cagaaactcg aaggcgagga gctttcgatc ctcgtgctga ccgacggcga
     2581 gacaattctg ccgctcgcga gcgcgcggga ccacaagcgt gcctatgaac atgaccgcgg
     2641 tcccaacacg ggcggcatgg gagcgtattc cccgagcgcg caattcgacg aggcctcgat
     2701 cggaaccatt atcgacaaga cggtgcggcc catcatcgac ggtctccgca aggaagggac
     2761 gccgtaccgc ggggttctct atgccgggct catgatgacg aaggacggtc cgtatgtcct
     2821 cgaatacaac tgccggttcg gcgatccgga gactcaggtg gtgcttccga tgctgcagtc
     2881 ggacttcggc gagatcatcg ccgccgcagt cgccaatcgc ctcgacgaaa tccggccccg
     2941 gtggtacaat ggctccggcg tttgcgtggt gctt
//
LOCUS       DAOOBX010000089         2966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_36964_length_2966_cov_4.712126, whole genome shotgun sequence.
ACCESSION   DAOOBX010000089 DAOOBX010000000
VERSION     DAOOBX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36964_length_2966_cov_4.712126"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /gene="rsmA"
                     /locus_tag="PLY30_01870"
     CDS             complement(<1..768)
                     /gene="rsmA"
                     /locus_tag="PLY30_01870"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPW64881.1"
                     /translation="MLNQVRLLKKYAIRVRGHLGQHLLMDPNTVRKIVDGLELAAGEP
                     VLEIGPGLGALTAEFLVRGHSVIAVEKDPKFVEILQTEIAPEFPGLLEIVRGDILKCD
                     LTKVLRERNVPLPVKAASNLPYYISTPILFHLIEHRACFHSAVLMMQKEVAARMTAKP
                     RTEDYGRLSVSSSVFGKTQTLFDVSPKCFLPAPEVTSRVIRYVFERGHVSGEEKALLD
                     VIRIAFSERRKTLLSLLSKQLRPEKGREELAMVFGRLG"
     gene            complement(761..1759)
                     /gene="pdxA"
                     /locus_tag="PLY30_01875"
     CDS             complement(761..1759)
                     /gene="pdxA"
                     /locus_tag="PLY30_01875"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPW64882.1"
                     /translation="MMRKKKQTPCVIAITMGDPGGVGPEVIVKSLRRHKLKSSCRYVV
                     IGSRRVFSFLEKKTGLSFPFATLPRDAEKLRGGKVYLRDMGGGTFDLAKESAANGRMA
                     VGAIATAADLAGRGLVQAICTAPLNKASARLAQKNFVGHTEFFAARSGTKKFAMMFVG
                     PRLKVTLATIHAPLKKVSGLLSAEKILEKIKLTAETLKRDFGLRKPGIAVCALNPHGR
                     ECGDEEDAVILPAVRRAVRAGIHAAGPFPADLLFHAAYEGKYDAVIGMYHDQALTAFK
                     LVHFHDGVNVTLGLPYVRTSPDHGTAYDIAYRGKANPSSMTAALELAERLALNRNA"
     gene            complement(1830..2795)
                     /locus_tag="PLY30_01880"
     CDS             complement(1830..2795)
                     /locus_tag="PLY30_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPW64883.1"
                     /translation="MKNLILALLILFVLVPFARAEEELLDRVVAVVNDEIITQAELDA
                     VLRPVYEDYKLQYSGEELAELVREARTKILGQLIEDKLVYQEAVAQDIVINEDEIEKE
                     LSDFRKRMENPDEFDAMLEREGMNMKSLREKFKRQLMIRRLQDMEIRSRVVISPAEIE
                     KFYRDNPDKFRKTARVKVRSLTVKKSPEAREKGIADEKAKERMDSLFLKLKQGENFEA
                     LVASSSEDSRAKEKEPSQWFEKGSMIESVDEAIFKTPKGHLTGIVETPVGFHIFKVED
                     VEEARQISFQEARDQISGYLFQVKSNERFREWTEQLKKTAYISIR"
BASE COUNT          571 a          880 c          856 g          659 t
ORIGIN      
        1 gccgagcctc ccgaagacca tggccagctc ctcgcgtccc ttttcgggcc ttaactgttt
       61 cgaaagaagc gaaagcaggg tctttcggcg ttcgctgaac gcgatgcgga tgacatcgag
      121 aagggccttt tcctcgccgg agacatgccc ccgttcgaac acataacgga tcacccggga
      181 cgtgacctcg ggggccggga gaaaacattt tggcgacacg tcgaagagcg tttgcgtctt
      241 cccgaaaacg gatgacgaaa cgctgaggcg tccgtaatcc tccgtgcgag gtttcgcggt
      301 cattcgcgcc gcgacttctt tttgcatcat cagcaccgcc gagtggaaac acgcgcgatg
      361 ttcgatcagg tggaaaagga tcggcgtcga gatgtaataa ggcaggttgc tcgcagcctt
      421 cacgggaagc gggacgttcc gctcccggag gaccttcgtc aggtcgcatt tgaggatatc
      481 cccccgaaca atctcgagca gtccgggaaa ctccggagcg atctcggtct gaaggatttc
      541 gacgaacttc gggtctttct cgaccgcgat cacgctgtgg ccgcgaacga gaaattccgc
      601 cgtcagggcg ccgagcccgg gaccgatctc gaggaccggt tcgcccgccg caagctcaag
      661 gccatccacg atcttgcgga ccgtgttcgg gtccataagg aggtgttgcc cgagatgccc
      721 gcggacgcgg atcgcgtatt tcttgagaag ccggacctga ttaagcatta cggttcagcg
      781 caagtctttc ggcgagttcc agcgcggcgg tcatcgagga cggattcgct ttccccctgt
      841 acgcgatgtc gtaggcggtg ccgtggtcgg gcgaggtcct tacataggga agaccgagcg
      901 tcacgttcac gccatcatga aaatgcacga gtttgaacgc cgtcaatgcc tggtcatggt
      961 acatgccgat cacggcatca tacttccctt cataagcggc gtggaaaaga aggtccgcgg
     1021 ggaacggtcc ggcagcgtgg attccggccc tcacggccct gcgaacggcg ggtaagatga
     1081 ccgcgtcttc ctcgtcgccg cactccctgc cgtgcgggtt cagagcgcag accgcgatcc
     1141 cgggctttcg gaggccgaaa tcccgtttga gcgtttccgc ggtgagcttg atcttttcca
     1201 gaatcttctc cgccgaaagc aggccgctga cctttttgag cggcgcgtgg atcgtggcca
     1261 gggtcacttt gagccggggc cccacgaaca tcatcgcgaa tttttttgtt cccgagcgcg
     1321 cggcgaaaaa ctcagtgtgc cccacgaaat tcttttgcgc gagccgtgcg gaggccttgt
     1381 tgagcggcgc cgtgcagatc gcctggacga gcccgcggcc cgcgagatcc gcggccgtcg
     1441 cgatggcccc gaccgccatc cgtccgttcg cggcactctc tttcgcgaga tcgaaagttc
     1501 cgccccccat gtcccgaaga tagaccttcc cgccccggag tttttccgcg tcgcgcggga
     1561 gtgttgcaaa agggaacgaa agaccggtct tcttttcaag gaaggaaaaa acccttcggg
     1621 acccgataac gacgtagcgg caggaagatt tcagcttgtg ccgccggagg gacttcacga
     1681 tcacttcggg gccgacgccg ccgggatcac ccattgtgat cgcgatcacg cacggcgttt
     1741 gtttcttttt tctcatcata gcctgttgga tgactcaaaa acgcggggcc cgggactggt
     1801 tccgggcccc gagggacgag ttcccttcct taccggattg aaatatacgc cgtcttcttg
     1861 agctgctccg tccactccct gaaccgctcg ttgctcttga cctggaacag gtaaccggag
     1921 atctggtcgc gggcctcctg gaaagaaatc tggcgggctt cctccacgtc ctcgaccttg
     1981 aagatgtgaa aaccgaccgg cgtttcgacg atcccggtca ggtggccttt cggggtcttg
     2041 aagatggcct cgtcaaccga ttcgatcatg gaaccctttt cgaaccactg ggaaggttcc
     2101 ttctccttcg cgcgggagtc ctcggaggaa ctcgcgacaa gcgcttcaaa attctcgccc
     2161 tgtttgagct tgagaaaaag cgaatccatc cgttccttcg ccttttcgtc ggcgatgcct
     2221 ttttcgcggg cctccgggct ctttttgacc gtgagcgaac gaaccttcac gcgggcggtc
     2281 ttgcggaact tgtcgggatt gtccctgtag aatttctcga tctccgccgg cgagatcaca
     2341 acacgggagc ggatctccat atcctgcaga cgacgaatca taagctgtct cttgaacttc
     2401 tcgcggaggc tcttcatatt catcccctcg cgttcgagca tcgcgtcgaa ctcgtcgggg
     2461 ttctccatgc gtttcctgaa gtcagagagt tccttctcga tctcgtcctc attgatcaca
     2521 atgtcctggg cgaccgcttc ctggtagacg agcttgtcct cgatcaactg gcccagtatc
     2581 ttcgtgcggg cttccctgac cagttccgcg agttcctcgc cgctgtactg cagtttgtag
     2641 tcctcgtaaa cggggcgaag gaccgcatcg agctcggcct gcgtgatgat ctcgtcattg
     2701 acaaccgcca cgacccggtc gaggagctct tcttcggcgc gggcgaaagg aaccagcaca
     2761 aacaggatca aaagcgcgag aatgaggttt ttcaccccgt tagggatcgg acacattggc
     2821 gttgccctta tgtttaagga ttctgatcag gcttttttca aatcatttct tcccggaagc
     2881 ggtagcgctg tttgaaacag gtcctgtctt caacggggtt cacagagcgg gtattataca
     2941 tcaaatggca cggcgtacga agtacg
//
LOCUS       DAOOBX010000090         2956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_37122_length_2956_cov_3.259221, whole genome shotgun sequence.
ACCESSION   DAOOBX010000090 DAOOBX010000000
VERSION     DAOOBX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37122_length_2956_cov_3.259221"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /gene="nusG"
                     /locus_tag="PLY30_01885"
     CDS             <1..361
                     /gene="nusG"
                     /locus_tag="PLY30_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPW64884.1"
                     /translation="RKFFPGYILVKMELNDESWYLVKNTPGISGFVGSGKRPLPLTDR
                     EVGAIIQEQEEKMTKPKPKVEFSEGESVRVKEGSFANFNGVIEQINPDRGKLRVMVTI
                     FGRPTPVELEYWQVEKV"
     gene            366..791
                     /gene="rplK"
                     /locus_tag="PLY30_01890"
     CDS             366..791
                     /gene="rplK"
                     /locus_tag="PLY30_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPW64885.1"
                     /translation="MAKEIQVKIKLQIPGGQANPAPPIGPALGQHGVNIMEFCKQFNE
                     RTKDRPGTVLPVLLTVYKDKSFTFILKNPPISAAIKKAVGLAKASSEPGRQTIGKLTK
                     EQAVEIAKAKMPDLNTTKIASAVKIVEGVARSMGIEVVK"
     gene            797..1486
                     /gene="rplA"
                     /locus_tag="PLY30_01895"
     CDS             797..1486
                     /gene="rplA"
                     /locus_tag="PLY30_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPW64886.1"
                     /translation="MMKRSKRYQKRSALVEPEKSYSLSEAVKILKKVEPGKADETISL
                     NFQLGIKAEAGDENVRGTVSLPHGSGRSVRVLCFTKGETIKDAEASGADIVGGDELVE
                     KVKNGFLDFDVVVAHPDMMREVSKLGRVLGPKGLMPTPKTGTVTPQIGKAVKELKAGR
                     IEFKSDKTAGLHAVCGKLSFSEEALLENARAVIKAVRDSKPPISKGEYLKSVTISATQ
                     GPGLKLNTGAL"
     gene            1491..2027
                     /gene="rplJ"
                     /locus_tag="PLY30_01900"
     CDS             1491..2027
                     /gene="rplJ"
                     /locus_tag="PLY30_01900"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPW64887.1"
                     /translation="MPSFEKELMFSEIIKEFEKSPYTFISSFQGLSVSDLTAARVSLE
                     KVARRSMVIKHAMARKIFEKMKFEGAEKFLKDSIIVTFGEKDPQDISKKIVDYAKAND
                     KWKAAGVIFEGQVHGEDFVKALAKLPSRKELLTQVVVRMKSPIAGVVNVLGALTRNLV
                     VTIAEIRKKKEAAPTAAA"
     gene            2073..2489
                     /gene="rplL"
                     /locus_tag="PLY30_01905"
     CDS             2073..2489
                     /gene="rplL"
                     /locus_tag="PLY30_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPW64888.1"
                     /translation="MTEATETKQISEKLQGILTSIETLTVLELADLVKALEDKFGVSA
                     AAAVPMGVVAMPGAAGGAAAPAEEQTSFTVVLVNSGDKKINVIKEIRTVTSLGLKEAK
                     DLVEGAPKTIKEGATKEEADKIKKLLEAQGAKVEIK"
     gene            2537..>2956
                     /locus_tag="PLY30_01910"
     CDS             2537..>2956
                     /locus_tag="PLY30_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPW64889.1"
                     /translation="MMPLIKRKSFAKIRDTMELPNLVAIQIRSYEEFLQTNRTGKRRK
                     RQGLEAAFADCFPIESFDGTCRLEYEGYALGKPKYSIPECQKRGMTYAAPLKVKLRLV
                     RKGVAKEQEVYIGELPLMTDTGTFIINGAERVIVSQLH"
BASE COUNT          750 a          771 c          877 g          558 t
ORIGIN      
        1 acgcaagttt ttccccgggt atattcttgt gaagatggaa ctcaacgacg agagctggta
       61 tctcgtcaag aacaccccgg gcatctcggg attcgtcgga tccggcaaga ggccgcttcc
      121 gctgactgac cgggaagttg gcgcgatcat ccaggaacag gaagaaaaga tgacgaagcc
      181 gaagcccaag gtcgagttct ccgaagggga gagcgttcgg gtcaaggaag gttcatttgc
      241 gaattttaac ggtgttatcg agcagatcaa tcccgatcgc ggaaaattgc gtgtgatggt
      301 cacgatcttc ggccgtccga cccccgtgga gctcgaatac tggcaagtag aaaaggtcta
      361 aaaacatggc gaaggaaatc caggtcaaga tcaagttgca gattcccggc ggacaggcca
      421 atcccgctcc gccaatcggc cccgcgctcg gtcagcatgg cgttaatatc atggagttct
      481 gcaagcagtt caatgagcgc acgaaagaca ggcccgggac ggtgcttccg gtcctgctga
      541 cggtctacaa ggacaagtcg ttcaccttca ttctcaagaa tccgccaata tcggccgcga
      601 tcaaaaaagc ggtcggtctc gcaaaggcct cctccgaacc gggccgccag actatcggca
      661 aactcacgaa ggagcaggcc gttgagattg ctaaggcgaa aatgccggat ctcaacacga
      721 cgaagatcgc aagtgcggtc aagatcgtcg aaggcgtggc ccgaagtatg ggcatcgaag
      781 tggtgaaata agaggaatta tgaagcgtag caaacggtat cagaaaaggt cagcgttagt
      841 cgaacccgag aaatcgtact cgctttccga agcggtcaaa atcctcaaga aggtcgaacc
      901 gggcaaagcg gacgaaacaa tatctctcaa ttttcagctg ggcatcaagg cggaagcggg
      961 tgacgagaac gtccgcggga ctgtcagcct tccgcacggt agcggccggt cggtgcgcgt
     1021 cctttgtttt acaaagggcg agacgatcaa ggatgccgag gcgtcaggag ccgacatcgt
     1081 cggcggtgac gagcttgttg agaaggtcaa gaacggtttc ctcgattttg atgtcgtggt
     1141 cgctcatccg gatatgatgc gtgaagtttc gaagctcggc cgcgtgctcg gtcccaaagg
     1201 tctgatgccg acgccgaaga ccgggaccgt gaccccccag atcggcaagg cggtcaagga
     1261 gcttaaggcc ggacgcatcg aatttaaaag cgacaaaacg gccgggcttc acgccgtttg
     1321 cgggaagctt tctttcagtg aggaagcgtt gctcgagaat gcccgggctg tgataaaggc
     1381 tgtccgtgat tcaaagccgc cgatcagcaa gggcgaatat ctcaagagcg tgacgatctc
     1441 cgcgacccag ggcccggggc tcaagctcaa cacaggagcg ttgtaattcc atgccttcct
     1501 tcgaaaaaga attgatgttc agcgagatca ttaaggaatt cgagaagagt ccgtacacgt
     1561 tcatctcgag cttccagggt ctgtcggtgt ccgaccttac cgccgctcgc gtcagcctgg
     1621 aaaaagtggc ccgtcgctcg atggtcatca agcacgcgat ggcccgcaag attttcgaga
     1681 aaatgaagtt tgagggcgcc gaaaaattcc tcaaggactc cattatcgtt acttttggcg
     1741 agaaagatcc gcaggatatc tcgaagaaga ttgtggatta tgcgaaggcg aacgacaagt
     1801 ggaaggccgc gggcgtgatc ttcgaaggtc aggtccacgg ggaagatttc gtcaaggcgc
     1861 tcgcgaagtt gccgagccgc aaggaactgc tcacgcaggt tgtggtccgg atgaagtcgc
     1921 cgatcgccgg ggttgtcaac gtcctcggcg cgcttacccg taacctcgtg gtgacgatcg
     1981 ccgagatcag gaaaaagaaa gaagcggctc cgacagccgc cgcataacca acagtcctta
     2041 tcaaaggaaa tcaatcaagg aggcaacagg tcatgacaga agcaaccgaa accaagcaaa
     2101 tctctgagaa acttcagggt attcttacct cgatcgagac cctcacggtc ctcgaactcg
     2161 cggatctcgt gaaggcccta gaagacaaat tcggcgttag cgccgcagcc gctgtgccga
     2221 tgggtgtcgt cgccatgccg ggcgccgccg gcggtgccgc tgcccccgcg gaagagcaga
     2281 cctctttcac ggtggtgctc gtgaactccg gtgacaagaa gatcaacgtg atcaaggaga
     2341 tccgcaccgt gacgagcctc ggcctgaaag aagccaagga cctcgtcgag ggcgctccga
     2401 agactatcaa ggaaggtgcc acgaaagaag aagctgacaa gatcaagaag cttctcgaag
     2461 cccagggcgc caaggtcgaa atcaagtaag cacgttccag tgtcaggcac atcattctaa
     2521 ggagggcatt cagctaatga tgcctttgat caagaggaag agcttcgcca agatccggga
     2581 tacgatggaa cttccgaacc tggtcgcgat ccagatcagg tcctatgagg aattcctgca
     2641 aacgaatcgt acgggcaagc gccgcaagcg ccaggggctc gaggcggcgt tcgcggattg
     2701 tttcccgatc gaaagcttcg acggaacctg tcgtcttgag tacgaggggt atgcgctcgg
     2761 gaaaccgaag tattccatcc ccgagtgtca gaagcgcggg atgacctacg ccgcgccgtt
     2821 gaaggtgaag ctccgtcttg tccggaaggg tgttgccaag gagcaggaag tttatatcgg
     2881 cgaattgccg ctgatgaccg ataccggcac cttcattatc aatggcgccg aacgtgtgat
     2941 cgttagccag ctccac
//
LOCUS       DAOOBX010000091         2952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_37175_length_2952_cov_3.865378, whole genome shotgun sequence.
ACCESSION   DAOOBX010000091 DAOOBX010000000
VERSION     DAOOBX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37175_length_2952_cov_3.865378"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLY30_01915"
     CDS             <1..351
                     /locus_tag="PLY30_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64890.1"
                     /translation="PTEGVVPMRHWTAASADPAVTESFRKKIAELKEANRLLSSENAE
                     LRSRNRELDAVCGSRPSKKERKKLEAKQAQLAVQSESFKKGQQEYFKKKSGGISGDAS
                     HDVSNVPGVVPVEE"
     gene            355..909
                     /locus_tag="PLY30_01920"
     CDS             355..909
                     /locus_tag="PLY30_01920"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPW64891.1"
                     /translation="MGSLPEKAALREQLLASRAAIPSPKRRLKSAKIFAKLFKTTAYR
                     KARRVAFYHGTADEVRTRPFLEKIIKEKELYLPRVGRTRRSLSLCRIRDLKRDLRKGT
                     YAIMEPRMHCAVLSASRMDLIVVPGVGFDRRGGRLGRGAGYYDRLLKKMPGVHKIGIC
                     YREQLVQNVPVAKHDIAMDQVITD"
     gene            923..2155
                     /gene="xseA"
                     /locus_tag="PLY30_01925"
     CDS             923..2155
                     /gene="xseA"
                     /locus_tag="PLY30_01925"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPW64892.1"
                     /translation="MKHEELIGERGGGRKIYTITELNKEVRGVLETRYAGVWVEGEVS
                     NYKRHSSGHIYLTLKDEQSQISAVFFSFHHRGLKFGLEDGLKVLAYGRASVYEARGQY
                     QFYIERVEAKGVGALQLAFQQLKEKLWKEGLFNEERKRPIPKFPKTVGVITSPTGAAI
                     HDILNVIGRRSGGTNVLLCPVKVQGDGAAEEVAGAIRMMNDFGEAEVLIVGRGGGSLE
                     DLWAFNEEPVARAIYGSKIPVISAVGHETDWTIADFVADLRAPTPSAAAELVVANRLD
                     LEERLRDRTERLRWGMRSLAEQHRQKLEYLGSSAAFKQPRYLLEQFAERTDELGRRLQ
                     GIFASWLALKRQVFATLTGRMNALNPIGILERGYSVSFAASGAVLKDAARVKTGEEII
                     TRLHKGKLVSRVIKTEKE"
     gene            2158..2451
                     /locus_tag="PLY30_01930"
     CDS             2158..2451
                     /locus_tag="PLY30_01930"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPW64893.1"
                     /translation="MAEKNEKVDFERSMQSLEKIVEALEGSELTLDEALKKYEEGVGL
                     VRICQSKLSEAEKKIEVLTKALDGSFKKEPFEAEGAAVKGRKKKRDTDDGDAF"
     gene            2482..>2952
                     /locus_tag="PLY30_01935"
     CDS             2482..>2952
                     /locus_tag="PLY30_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPW64894.1"
                     /translation="MKYERFFKKQLRAIEKELRRIMKTRQARPRSIHRVMQYAVFTGG
                     KRFRPVLTLAVCEACGGKMRDVLPAACAIEMIHTYSLIHDDLPALDNDDLRRGQPTCH
                     KKFGEAAAILAGDGLLTLAFEVLSEVKPAERAAELVRRVSTASGTHGMIGGQVED"
BASE COUNT          690 a          759 c          952 g          551 t
ORIGIN      
        1 cccacggagg gagtcgtccc gatgaggcat tggacggccg cctcggccga tcctgccgtg
       61 accgaatctt tcaggaaaaa gattgctgag cttaaagaag caaaccgcct gctctcctcc
      121 gagaacgcgg agcttcggtc caggaaccgt gagctggatg ccgtttgcgg ttccaggccc
      181 tcgaagaagg aacgtaaaaa actggaagca aaacaggctc agcttgccgt gcagtcggaa
      241 tcgttcaaaa aaggccaaca ggaatatttc aaaaagaaat ctggcgggat ttccggcgat
      301 gcgtcccatg atgtttcgaa tgttccgggt gtcgtgccgg tcgaagaata ggacatgggg
      361 agtttgccgg aaaaggccgc tctgcgggaa caactgcttg ccagtcgcgc ggccatcccg
      421 tccccgaagc gtcgcctgaa gagcgcgaag atcttcgcga agcttttcaa gacgacggct
      481 tatcgaaagg cccgcagagt cgctttctac cacggaaccg cggatgaggt ccggacgcgg
      541 ccattccttg aaaagatcat taaggagaag gaattgtatt tgccgagggt cggaaggacc
      601 cggcggtcac tttccctgtg ccgtatccgt gatctgaaac gcgacctccg aaaagggacg
      661 tacgcgatca tggagccccg gatgcactgc gcggtcctgt cggcatcccg gatggacctt
      721 atcgttgtgc ctggtgttgg cttcgaccgg cgcggcggac ggctcggccg cggagcgggt
      781 tactatgacc gcctcctcaa aaaaatgccg ggcgttcaca agatcgggat ctgctaccgg
      841 gaacagcttg tccaaaacgt acccgtcgca aaacacgata tcgcaatgga ccaggtcatt
      901 acggactgag agccaggaaa cgatgaaaca cgaagagttg atcggggaac gagggggcgg
      961 ccggaagatc tatacgatca cggagctcaa taaagaggtc cgtggagtgc tcgaaacgcg
     1021 ctacgcgggc gtgtgggtgg aaggcgaggt ctcgaattac aagcgccata gctccggcca
     1081 tatctacctt accctgaaag acgagcagtc gcagatttcc gcggttttct tcagctttca
     1141 ccaccgcggc ctgaagttcg ggctcgagga cgggcttaag gtgcttgcct acggccgcgc
     1201 gtcggtttat gaggcgcggg ggcaatacca gttctacatc gaacgcgtcg aagcgaaagg
     1261 ggtcggagcg cttcagctcg cattccagca actcaaagaa aaattatgga aggaagggct
     1321 cttcaacgag gagcgaaagc ggcccattcc gaagttcccg aaaacggtcg gggtcatcac
     1381 gtcaccgaca ggagcggcga tccacgatat cctgaacgtc atcggacgca ggtccggcgg
     1441 gacgaacgta ctgttgtgtc cggtcaaggt gcagggggat ggcgccgccg aagaggttgc
     1501 gggggctatc cggatgatga atgatttcgg agaagcggaa gtgctgatcg tgggccgcgg
     1561 cggaggaagc ctcgaggatc tctgggcctt caacgaggag ccggtcgcgc gggccattta
     1621 cggctcgaag attcccgtga tctccgcggt ggggcatgag acggactgga cgatcgcgga
     1681 ttttgtcgcg gatctccggg ctccgacgcc gtcggccgcg gccgagctgg tagtcgcgaa
     1741 ccgtctggac cttgaagaga gattgcggga ccggacagag cggttgcgtt gggggatgcg
     1801 gtcactggcg gagcaacatc gtcagaagct tgagtattta ggttcgagcg cggcttttaa
     1861 acaacctcgc taccttctgg agcagttcgc ggaacggacg gatgaactgg ggcggcggct
     1921 ccagggaatt ttcgcgagct ggctcgcgct caaaaggcag gtttttgcga cgctgacggg
     1981 gcggatgaac gcattgaacc ctatcggaat cctggagcgt ggttattcgg tttcgttcgc
     2041 ggcttcgggc gcggtcctga aagatgccgc ccgcgtcaaa acaggggagg agatcatcac
     2101 gcggttgcat aaaggcaaac tggtttccag ggtcatcaaa acggaaaagg aatgaatatg
     2161 gcggaaaaaa acgagaaagt ggatttcgaa aggtcaatgc agtcgcttga gaagatcgtc
     2221 gaagcgcttg aaggtagcga gttgacactc gatgaggctc tcaaaaaata cgaggaaggc
     2281 gtcggtctgg tccgcatctg ccaatccaag ctcagcgagg ctgagaaaaa gatcgaagtc
     2341 ctgaccaagg cgcttgacgg gtccttcaag aaggaacctt tcgaggctga aggggccgcc
     2401 gtgaaaggcc ggaagaaaaa gcgggatacg gatgacgggg acgctttctg atcgcgcgtc
     2461 agatcttgtg agagaaaagc catgaaatac gaaagattct tcaaaaaaca gctgcgggcg
     2521 atcgaaaaag agcttcgacg catcatgaaa acacgccagg ccaggccccg ttccatccac
     2581 agggttatgc aatacgctgt ttttaccgga gggaaaagat tccgtccggt ccttaccctg
     2641 gcggtttgcg aagcctgcgg cggtaaaatg cgcgacgtgc ttcccgcggc ctgcgcgatc
     2701 gagatgatcc acacgtattc gctgatccat gacgaccttc cggcgctcga caatgacgat
     2761 cttcggcgcg gtcaaccgac ctgtcacaag aagttcggcg aagccgcggc gatcctggcg
     2821 ggggacggcc ttctgacgct ggcctttgag gtcctctcgg aagtgaaacc ggcggaacgg
     2881 gccgcggagc ttgtaaggcg ggtgtcgacc gcttccggaa cgcatggtat gatcggcggg
     2941 caggttgagg ac
//
LOCUS       DAOOBX010000092         2941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_37373_length_2941_cov_2.988219, whole genome shotgun sequence.
ACCESSION   DAOOBX010000092 DAOOBX010000000
VERSION     DAOOBX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37373_length_2941_cov_2.988219"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="PLY30_01940"
     CDS             <1..1106
                     /locus_tag="PLY30_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HPW64895.1"
                     /translation="TTDVLEKRIAQFDGGVGALAVSSGQSAIALALLNIAQAGDEIVS
                     ADNLYGGTYNLLHYTFARFGIKVRFVKSNDLQGIQKAITPKTKAVYAESIGNPKLDVA
                     DLEGIAKVAHRNGLPFVLDNTVSPYLLRPFDHEADIVVYSATKFIGGHGTSLGGLIVD
                     SGKFDWTNGKFPLIAGPDPSYHGINFVEALKPLGNIAYITKARVTLLRDLGNALSPFN
                     SFLFLQGFETLHLRMPRHSENALTVAKHLEKNPKVAWVHYPGLASSPEKERARKYLPK
                     GAGAIIGFGIKGGLRAGRKFIDSLGLISHLANIGDAKTLAIHPASTTHQQLSEEERLA
                     TGVTPDFIRLSVGLEHIDDILADIDQALEKASA"
     gene            1325..2257
                     /gene="cysK"
                     /locus_tag="PLY30_01945"
     CDS             1325..2257
                     /gene="cysK"
                     /locus_tag="PLY30_01945"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPW64896.1"
                     /translation="MGIYADVTKAVGDTPLIRLNRLTEGLRTEVLVKLESANPMSSVK
                     DRTGVAMIDAAEKTGKLRSGATIVEPTSGNTGIALAFVAAARGYKLILTMPETMSVER
                     RQLLRILGAEIVLTEGAKGMRGAVEKAAEIQASTPDSFMPQQFNSPANVEVHRKTTAE
                     EIWRDTDGKVDVFVSGVGTGGTLTGVGEVLKKRNPSVKIVAVEPADSPVLSGGKPGLH
                     KIQGIGAGFVPGVLNTTLYDEVLKVRSEDAGETARQLARQEGILGGISSGANVWASLE
                     IAKRPESKGKTIVTVICDTGERYLSTWLFEEYAK"
     gene            2245..>2941
                     /locus_tag="PLY30_01950"
     CDS             2245..>2941
                     /locus_tag="PLY30_01950"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HPW64897.1"
                     /translation="MREVTKGIEAVETKYFTMAHEFRLESGRTIVPVTLAYETYGLLN
                     DKKDNAVLVMHAFTGDAHAAGSHKGDKGKGWWDFMIGPGRALDTDRYFVICINVLGGC
                     KGSTGPASVNPKTGKPYGLDFPVVTLGDMVNAQVKLIDHLGIDKVYAVLGGSMGGMLA
                     LQWAALHPERVKCVIPIAATLKHSPQQIAFNEVGRQAIMADPAWNGGDYYGKAIPEHG
                     LAVARMLGHITYMS"
BASE COUNT          624 a          787 c          958 g          572 t
ORIGIN      
        1 cgacaaccga cgttctcgaa aaaagaattg cgcagttcga cggcggggtg ggagctctcg
       61 cggtttcgag cggccagtcg gcgatcgcgc tcgcgctgct caatatcgcg caggcggggg
      121 atgagatcgt ttccgcggac aacctttacg gcgggaccta taacctgctc cattacacgt
      181 tcgcgcgttt cggcatcaag gtcaggtttg tgaaatcgaa cgacctgcag ggaatccaga
      241 aggcgatcac gccgaagacg aaggccgtgt acgcggagtc gatcgggaac ccgaagctcg
      301 acgttgcgga tctcgagggg atcgcgaagg tcgcgcacag gaacggtctt ccgtttgttc
      361 tcgataatac cgtgtcaccg tatctcctgc gtcccttcga tcacgaggcg gacatcgtcg
      421 tgtattctgc gacgaaattt atcgggggac acggtacgtc gctcggcggt cttatcgtgg
      481 attccggaaa attcgactgg acgaacggga aattcccgct gatcgccggt ccggacccga
      541 gctatcatgg catcaatttc gtggaagccc tgaaaccgct tggtaatatc gcctacatca
      601 ccaaagcgag ggtgacgctc ctgcgggacc tcgggaatgc gctttctcct ttcaattcgt
      661 tcctgttcct gcagggtttt gagacattgc acctcaggat gccgcggcac tcggagaacg
      721 ctcttacggt cgcgaagcac ctcgagaaga acccgaaagt ggcgtgggtc cattatcccg
      781 gtcttgcgtc gagccccgag aaggaacgtg cccggaaata ccttccgaaa ggtgccggcg
      841 cgatcatcgg gttcggtatc aagggcgggc tcagggcggg ccggaaattt atcgactcgc
      901 tcggactgat ttcgcatctt gcgaatatcg gggacgcgaa aacgcttgcg atccatccgg
      961 cgagcacgac gcatcagcag ctttccgagg aggagcgtct cgcgaccggc gtcacgcccg
     1021 atttcattcg tttgtcggtc ggcctcgaac atatcgatga tattctcgcg gatatcgatc
     1081 aggccctcga aaaagcgagc gcctgagcca ggagaagata tcgcggatgg ggaagccatc
     1141 atgccggaca agcatggtct caaggtcaac atcctgaaga ccacggaccg tgtactgtca
     1201 ggaacgcaca aggttggtgg ggacgcgtac cttgttgcgg cgaaactgtt gcttcacccg
     1261 gttcggcgcc ggattcacgg tgaacgcgca ttacgcgttt taaaatcatc acgggaggtt
     1321 tcttatgggc atttatgcgg atgtgacaaa ggcggttggc gatacccctc tcatcaggct
     1381 taaccgcctg acggaggggc tccgcaccga ggtgttggtg aagctcgagt ccgcgaaccc
     1441 gatgtcgagc gtgaaagacc ggaccggggt cgcgatgatc gatgcggccg aaaaaaccgg
     1501 caaactccgt tcgggcgcga cgatcgttga gccgacgagc gggaacacgg ggatcgcgct
     1561 cgcgtttgtc gcggcggccc gcggctataa actgattctc acgatgccgg agacgatgag
     1621 cgttgagcgc cgccaattgc tcaggattct cggggcggag atcgtgttga ccgaaggcgc
     1681 gaaaggaatg cgcggcgcgg tcgagaaagc cgccgaaatc caggcgagca cgccggacag
     1741 tttcatgccc cagcagttca atagcccggc gaatgtcgag gtccaccgga aaacgacggc
     1801 tgaggagatc tggcgcgata cggatggcaa ggttgatgtg tttgtgtcgg gggtcggaac
     1861 gggagggacg ctgacgggtg tgggagaagt gctgaaaaaa aggaaccctt ctgttaaaat
     1921 cgtggccgtt gaaccggcgg attccccggt gctttcgggc gggaaacccg gcctgcacaa
     1981 gattcagggg atcggcgccg ggttcgtgcc gggcgttctg aacacgacgc tctatgacga
     2041 ggtcctgaag gtccgcagcg aagacgcagg ggaaacggcc cgccagcttg cgaggcagga
     2101 gggtattctc ggcggtatct cgtcgggcgc gaacgtttgg gcgtcgcttg agatcgcaaa
     2161 gcggcccgaa tcgaaaggga aaacaatcgt gaccgttatt tgcgacacgg gcgaacggta
     2221 tctttcaaca tggctgtttg aggaatacgc gaagtgacaa aaggcattga ggcggtcgag
     2281 acgaaatatt tcacaatggc gcatgagttc cggctcgagt cagggcggac gatcgtgccc
     2341 gtgacgctcg cctatgagac ctacggcttg ctgaacgata aaaaggacaa cgcggtcctc
     2401 gtgatgcacg ccttcacggg ggatgctcac gcggccggtt cccacaaggg ggacaagggc
     2461 aagggttggt gggattttat gatcggcccg ggacgcgctc tcgatacgga tcggtatttc
     2521 gtgatctgta tcaatgtgct tggcggttgc aaaggaagca cgggccccgc gtcggtcaat
     2581 ccgaagacag gcaagcccta cggcctggat ttcccggttg tgacgctggg ggacatggtg
     2641 aacgcccagg tgaagctgat cgatcatctc gggattgaca aggtttacgc cgtgctcggc
     2701 ggttccatgg gcggcatgct ggcgctccag tgggccgcgc ttcatccgga gcgggtgaaa
     2761 tgcgtgatcc cgatcgcggc gacgttgaag cactccccgc aacagatcgc tttcaacgag
     2821 gtcggtcgcc aggccatcat ggctgatccg gcgtggaacg gcggcgatta ttacgggaag
     2881 gcgatcccgg agcacggtct tgccgtcgcg cgaatgctcg ggcacatcac ctacatgagc
     2941 a
//
LOCUS       DAOOBX010000093         2929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_37562_length_2929_cov_3.373695, whole genome shotgun sequence.
ACCESSION   DAOOBX010000093 DAOOBX010000000
VERSION     DAOOBX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37562_length_2929_cov_3.373695"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="PLY30_01955"
     CDS             complement(<1..1536)
                     /locus_tag="PLY30_01955"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPW64898.1"
                     /translation="MKPERKIYIFDTTLRDGEQSPGASLLPEEKLKIAKQLECLNVDV
                     IEAGFPASSEGEMRAVAMIAEHVRKPVICGLSRMIPRDIDACRSALEGAAKKRIHVFL
                     ATSQIHREFKLKKGKREIIEMAVKNIRSARKDFRQVEFSPEDASRTERDFLVDVVRAA
                     IDAGATSINIPDTVGYAIPEEFSDLIRLLIRKNPALGKQIVLSVHCHNDLGLATANSI
                     AAVLAGANQIECTVNGIGERAGNASVEEIAMILNTRRDFMGCYTDIKLSEITKSSRLV
                     SHLTGMVVQPNKAIVGRNAFWHASGIHQDGILKKRQTYEIMNPKKIGLSGNQLMLGKL
                     SGKHAFAVRVKKLGFNLSAAEIDAAFVKFKTLADKKKYIFDEDIEAILQNQIAQVPET
                     WKLVSMKVVSETGQKPSATIRLSYQGQVSEASSTGDGPVEACYKAIETVVRSGAQVAH
                     YGLQSVTGGKDALGDVVVKLVVGTKEVSGRGTSTDVIEASVRAYLFALNKLCQEPKRG
                     SASA"
     gene            complement(1640..>2193)
                     /gene="ilvC"
                     /locus_tag="PLY30_01960"
     CDS             complement(1640..>2193)
                     /gene="ilvC"
                     /locus_tag="PLY30_01960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPW64899.1"
                     /translation="CLIAIAQDYTGKAKETALAYAMAIGGARAGVIETNFREETETDL
                     FGEQAVLCGGLSELITAGFDTLVEAGYQPEMAYFECLHEVKLIVDAMFVSGISGMYKR
                     VSDTAEYGGYSRGPRVITKSTRKEMKKILKEVVNGKFAKEWIGENRKGRPNFKKMRAT
                     CEKHKIENVGERLRKMMDFINKK"
     assembly_gap    2194..2203
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2204..2777)
                     /gene="ilvC"
                     /locus_tag="PLY30_01965"
     CDS             complement(<2204..2777)
                     /gene="ilvC"
                     /locus_tag="PLY30_01965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPW64900.1"
                     /translation="MAVKIYYDKDADLNLLKGKKVAIIGYGIQGRGQGLNLRDSGVDV
                     LVSELQGTPNYDMAIKDGFKPVSAADAAKQADIIQILTQDHLQAEIYEKHIAPNLKAG
                     KALCFSHGFNIHFKYIKPASNIDVFMVAPKGPGSLVRSQFVEGGGVPCLVAVQQNPSG
                     KALKIGLAYAKAIGGTRAGVIETTFKEETET"
BASE COUNT          505 a          904 c          821 g          689 t
ORIGIN      
        1 cgcggacgcc gaaccccgtt tcggttcctg acacagtttg ttcagggcaa aaagatatgc
       61 gcggacgctc gcctcgatga cgtcggtgct cgtaccgcgg ccggaaactt ctttcgtccc
      121 gaccacgagc ttcaccacca catcgccgag cgcgtccttg ccgcccgtca cggactgcag
      181 gccatagtgg gcgacctggg cgccggagcg gacgaccgtt tcgatcgcct tataacacgc
      241 ttcgacggga ccgtccccgg tggaactcgc ctcgctcacc tgtccctgat aggaaagccg
      301 gatcgtggcg gatggtttct gccccgtttc ggagacgacc ttcatggaaa cgagcttcca
      361 cgtctccggg acctgggcga tctggttctg caggatcgct tcgatgtcct cgtcaaaaat
      421 atactttttc ttgtccgcga gcgtcttgaa cttcacgaag gcggcatcga tctccgccgc
      481 ggacagatta aacccgagtt ttttcacgcg caccgcgaac gcgtgcttcc cggaaagctt
      541 gcccagcatc agctgatttc ccgagagacc gatcttcttc ggattcatga tctcgtaggt
      601 ctggcgcttc ttgagaatcc cgtcctgatg gataccggag gcgtgccaga aggcgttcct
      661 cccgacgatc gccttgttcg gctggacgac catgcccgtc agatgcgaca caagacgcga
      721 ggacttcgta atctcggaca gcttgatatc cgtgtaacaa cccatgaaat cccggcgcgt
      781 gttgaggatc atcgcgatct cttcgaccga cgcgttgccg gcccgctcgc cgatcccgtt
      841 cacggtgcac tcgatctggt tcgcgccggc cagcacggcg gcgatcgagt tcgcggtcgc
      901 aagaccgaga tcgttgtggc agtggaccga aagcacgatc tgcttgccaa gcgcggggtt
      961 cttcctgatc aggagccgga tgaggtccga gaactcctcg gggatcgcgt agccgacggt
     1021 atcggggata ttgatcgagg ttgctcccgc gtcgatcgcg gcgcggacga catcgacgag
     1081 aaaatcccgt tccgtgcgcg atgcgtcctc gggagagaac tcgacctgac ggaaatcctt
     1141 cctggcggag cggatgttct tcaccgccat ctcgatgatc tcgcgtttgc ccttcttgag
     1201 cttgaattcc cggtgaatct gcgatgtcgc gaggaagaca tgaatccgtt ttttcgccgc
     1261 cccttcgagt gcactccggc aggcgtcgat atcccgcgga atcatgcggg aaagaccgca
     1321 gatcacgggt ttcctcacgt gctccgcgat catggcgacc gccctcatct caccttccga
     1381 ggaggcgggg aaccccgcct cgatcacatc gacgttcagg cactcgagct gcttcgcgat
     1441 cttgagcttc tcttcgggca ggagggacgc gcccggcgat tgctcgccgt cccggagcgt
     1501 cgtgtcgaat atgtatattt tccgttctgg tttcatgacg tcaggccgta ctgttccgga
     1561 tacacgccgc aagccgcgga ccacacgaaa ttcgtcctgt ctctcttgtg gccttgcggc
     1621 tttgcggctc ggttatctgt tacttcttgt tgatgaaatc catcattttg cggaggcgtt
     1681 ctccgacatt ttcgatcttg tgcttttcgc aggtcgcacg cattttcttg aagttcggac
     1741 ggcccttccg gttctcgccg atccattcct tcgcgaactt tccgttcacg acctccttga
     1801 ggatcttctt catctccttg cgcgtggatt tcgtgatcac gcgggggccg cggctgtaac
     1861 cgccgtactc ggccgtgtcg gaaacgcgtt tgtacatacc gctgatcccg gacacaaaca
     1921 tcgcgtccac gatcagcttc acttcgtgga gacattcgaa atacgccatc tcgggctgat
     1981 agccggcttc cacgagcgtg tcgaaccccg ccgtgatgag ctcgctcagg ccgccgcaga
     2041 gaaccgcctg ttctccgaag aggtctgttt cggtttcttc gcggaagttg gtctcgatca
     2101 cgccggccct cgccccgccg attgccattg cgtaggcaag cgccgtttcc tttgcctttc
     2161 cggtgtaatc ctgcgctatg gctataaggc aggnnnnnnn nnnccgtttc ggtctcttcc
     2221 ttgaatgttg tttcaatcac gccggcgcgc gtcccgccga tcgccttcgc gtacgcaagt
     2281 ccgatcttga gcgctttacc gctcgggttc tgctggaccg ccacgagaca cggcacgccg
     2341 ccgccttcga cgaactggga gcgcacgagc gaacccgggc ccttcggggc gaccataaac
     2401 acgtcgatat tcgacgcggg cttgatgtac ttgaaatgga tgttgaaccc gtgcgagaaa
     2461 caaagcgcct tgccggcctt cagattcggg gcaatgtgtt tctcatagat ctcggcctga
     2521 aggtggtcct gggtcaggat ctggatgata tcggcctgct tcgccgcgtc ggccgcggag
     2581 accggcttga aaccgtcctt gatcgccatg tcatagttgg gcgtcccctg gagctccgag
     2641 acgagaacat cgacgcccga gtccctgagg ttcagcccct gtccgcggcc ctggatgccg
     2701 tagccaatga tggcgacttt cttgcctttg agaaggttga ggtccgcatc cttgtcgtaa
     2761 taaatcttga ctgccatgtc tgttttcctt tctggttgtg aataaaaagc gttacttttt
     2821 cccggaagct tttttcgacc gggatttgcc cgcggctttc ttggacggcg ccttgagctc
     2881 atgacgccgc gccatcgcga tctgtcccgt ccgcaccact tcgcgaaga
//
LOCUS       DAOOBX010000094         2921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_37702_length_2921_cov_2.959177, whole genome shotgun sequence.
ACCESSION   DAOOBX010000094 DAOOBX010000000
VERSION     DAOOBX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37702_length_2921_cov_2.959177"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..328)
                     /locus_tag="PLY30_01970"
     tRNA            complement(242..328)
                     /locus_tag="PLY30_01970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(292..294),aa:Ser,seq:tga)
     gene            complement(424..942)
                     /locus_tag="PLY30_01975"
     CDS             complement(424..942)
                     /locus_tag="PLY30_01975"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPW64901.1"
                     /translation="MISRGVEKPDHQHFMKEAIKQAVTALEKGEVPVGAVVVWKNRIV
                     ARAHNQMEMLRDPTAHAEMIALTQASETLASLEEARPRNRDHRGSLEGATLYVTLEPC
                     PMCAGALVVTKCTNLVFGARDPRAGSCHSLYHITEDDRLNHQVKVTGGVMADDAKFLL
                     SEFFKGLRKEKR"
     gene            1161..2414
                     /locus_tag="PLY30_01980"
     CDS             1161..2414
                     /locus_tag="PLY30_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW64902.1"
                     /translation="MLPSISQKPRISEWLPLATVFATVYFFSMNGLGALPSLAINFLL
                     KDRIGLTPAQLSYFQAVTLIAWIVKPLWGMISDLCPILGSRRRSYLVLTSLFAAVSWL
                     VLSVAPSYTAGLLLTVMTLITLAYAFQDVVSDGLMVESGQRLDMTGQFQSIQWTAVYI
                     AMIITSFAGGIIADLAKAGSLSYRLIFAGTSLFPMLTAVVSAWFVREPAENQPERMAG
                     LNLRNIFRRKGIWILSFFLFLWNFSPSFGAPFFYYAVDTLKFTGYFLGILQAVAGVGA
                     LLGSVLYGFFFARIPLKKFLFAAILLGVAATLSYYIYFAPGLIAIPGLLKGLALTLNF
                     LLGMASSVIFLALLNLAAKASPRFAGGTVFALLMSFYNLGQMGSSALGGFLLPLVGLQ
                     PLIAISAAVSLLVLFLLPYLPVPGD"
     gene            complement(2510..>2921)
                     /locus_tag="PLY30_01985"
     CDS             complement(2510..>2921)
                     /locus_tag="PLY30_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HPW64903.1"
                     /translation="GYIFNFGKEKTVHVTPFTELDYGYLYRNLSKVKGLGVNDYKVKG
                     EGATDLEQGFGVQIAKSFVSELGTFIPYVKGEWLIAYVRDRQSENRQRNSGLFGAGLI
                     FLNKGHVTTSVNWDMLVQNRDLENVGYGKIRYDF"
BASE COUNT          540 a          912 c          721 g          748 t
ORIGIN      
        1 accgatctcc ttgtcgcgaa tggtacaaaa ataccgggtc accgcgtaaa catccacgcg
       61 gacgaatcgc cgcctttcgt gatgccttgt ttcctgtgag tgctgggaca tcaaacaaac
      121 tccgttttgt gataatacca tgacgaacat actattcatc ggccgcggag cttttgtctt
      181 gaacggattc ccggaaattt ccggggtctg ttcgcgcacc cgcaatccga tgtattaaca
      241 acggagggag taggattcga acctacgaga cgcttgcgcg cctaacggtt ttcaagaccg
      301 ccgcattcaa ccgctctgcc atccctccat tgagaactca ttccgggaca aatcttaaag
      361 agcatttctc gccgcccgcc acaaaaatga ctttcgcgtg ccctgcggca tgtgactttg
      421 tgtctatcgc ttctctttcc gcaacccctt gaagaattcg gaaagaagga acttcgcgtc
      481 atccgccatc acgccacccg tcaccttgac ctgatggttc aaccggtcgt cttccgtaat
      541 atgataaagg ctgtggcaag accccgcccg ggggtcgcgg gcgccgaaca cgaggttcgt
      601 gcattttgtc acaaccagcg cccccgcgca catcgggcac ggttcgagcg tcacataaag
      661 cgttgcgcct tcaagcgatc cgcggtgatc gcgattccgg ggcctcgctt cttccaggct
      721 cgcgagtgtc tccgaggcct gcgtaagggc aatcatttcg gcgtgcgccg tcgggtcccg
      781 gagcatttcc atctgattgt gggcgcgcgc cacaatccgg ttcttccaca cgaccacggc
      841 gccgaccggc acctctcctt tttcgagggc cgtgacagcc tgcttgatcg cttccttcat
      901 aaaatgctga tgatcgggct tttccacccc cctagagatc atggcaaacc tttctttcgc
      961 aaagaacggc gaaggcacag tcacgcgtat tcgcctcttt gttttcggaa gcactcctga
     1021 agcgctttct gtctctaacg ggatccatgg cgggtattat tatacataaa gccccgggcg
     1081 ggtcatcccg gaagacagga actctcgatt ttcacgcaaa acaagtgccc ccgttttggt
     1141 ttcctgctat ccttaccgcc atgcttccgt cgatctctca aaaaccgcgc atctctgaat
     1201 ggcttcctct cgcgaccgtt ttcgccacgg tgtatttttt ctccatgaac ggactcggag
     1261 ccctccccag cctcgcgatc aattttcttc tgaaggaccg cattggtctc acgcccgcgc
     1321 agctttccta cttccaggcc gtgacgctga tcgcgtggat cgtcaaaccc ctctggggca
     1381 tgatctcgga cctctgcccc attctgggat cgcgccgaag atcctatctc gtgcttacgt
     1441 cactttttgc cgcggtttcg tggctcgttc tttcggtcgc gccttcatat acggctggtc
     1501 tgctcctcac cgtcatgaca ctgatcaccc tggcctacgc cttccaggat gtcgtctcgg
     1561 acggcctcat ggtcgaatcg ggacaacggc tcgacatgac cggccagttc cagtcgatcc
     1621 agtggaccgc cgtctatatc gcaatgatca tcacgtcttt cgcaggcggg atcatcgcgg
     1681 acctcgcgaa ggccggttcc ctttcctacc ggctcatttt tgcggggacc tcgcttttcc
     1741 cgatgctaac cgcggtcgtt tcggcgtggt tcgtcaggga accggccgaa aaccagccgg
     1801 aacggatggc cggactcaac ctgcggaata tcttccgccg caaaggaatc tggatcctct
     1861 ctttcttcct cttcctctgg aacttttctc cgtccttcgg ggcgccgttt ttttattacg
     1921 ccgtcgacac cctgaagttt acgggctatt ttctcggaat ccttcaggct gtcgcagggg
     1981 tgggggcact tctcggaagt gtgctttacg gctttttttt cgcgcgaatc ccgctcaaaa
     2041 aatttctgtt cgcagccatt ctgctcggcg tcgccgcgac actttcctat tatatttatt
     2101 ttgcgcccgg gctgatcgcc atccccgggc ttttgaaggg gctggctctc acattgaatt
     2161 tcctgttggg gatggcaagc tcggtgatct tcctcgccct gctcaatctc gcggcaaaag
     2221 cctccccgcg cttcgcggga ggaacggtct tcgcgctcct catgtcgttc tacaacctcg
     2281 ggcagatggg ctcaagtgcc ctcggcggtt ttcttttgcc gctggtggga ctccagccgc
     2341 ttatcgccat ctccgcggcc gtctccctcc tcgtgctttt cctgctgcct tatcttcctg
     2401 ttcccggaga ctgacccttt tggatatccg cggctcgtca tagcccgtgc agacaaaaaa
     2461 agacccggtc ttcggttcga agaccgggtc ttttttgatc catccgttct tagaagtcgt
     2521 agcgaatctt cccgtacccg acgttctcga gatcgcggtt ctgcaccagc atatcccagt
     2581 taaccgacgt cgtcacatgt cccttgttca gaaagataag cccggcgccg aacagaccgc
     2641 tgttacgctg gcgattctcg ctctgccggt cgcgaacata ggcgatcagc cattcaccct
     2701 tgacgtacgg gatgaaagtg ccaagctccg aaacgaagga cttcgcgatc tgcacgccaa
     2761 atccttgttc gaggtcggtc gcaccttccc ctttgacctt gtagtcgttc acgcccaggc
     2821 ccttgacttt ggaaaggttc cggtagaggt agccgtaatc aagttccgta aaaggcgtca
     2881 catgcacggt tttttcttta ccaaaattga aaatgtagcc g
//
LOCUS       DAOOBX010000095         2913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_37829_length_2913_cov_2.783065, whole genome shotgun sequence.
ACCESSION   DAOOBX010000095 DAOOBX010000000
VERSION     DAOOBX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37829_length_2913_cov_2.783065"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="PLY30_01990"
     CDS             <1..1900
                     /locus_tag="PLY30_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPW64904.1"
                     /translation="AAGEMRARVERLTNQQVWISTFHSTCLRILRMDGAAIGLDPHFL
                     VYDESDQLQLIKECIRELSFDEKKVHPKGVKEEIQRAKDYLRTPCQFAEKAMDVYQDA
                     VAKVYMLYEEKMRRLKACDFGDLIMKTVALYDRAPEVLKAWQSRFRYILIDEYQDTNH
                     AQYRFAGLLAAARKQITVVGDPDQSIYAWRGADIRNILNFEADYPECGVIRMEQNYRS
                     TSVILDAANHLIRNNQMRKPKDLWTERKGGEPIELYEAEDEVDEAQYVVSNILRHRSA
                     GRKLADQVVFYRVHAQSRVIEDHLRRANIPYKIVGGVRFYDRKEIRDVIAYLRLVAYP
                     TDELSLKRVINVPARGIGKKAMETLEAEVSRAAGDVSSWDSVLADVAKIGGLAPRVKK
                     NLTEFYGMIKRLRTESRELPVRDILERVLEESRYIAELEAEHTAEAEARVGNIEEFFS
                     VIDDFVQSGGGNGAGIFGLTGDGPQAAVSGPRLSPLELFLESIELVTDLDQWDQGTNV
                     LTLMTLHTAKGLEFPVVHMVGLEEGVFPHVNSYLGSADEIEEERRLCYVGITRAREKL
                     HISYASARRLYGAIHHNLPSRFLTEIPPHLLRAVRGSGSSSEYRDDDRDEFPDSRIED
                     DPVIDFDF"
     assembly_gap    2221..2230
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2278..>2913
                     /locus_tag="PLY30_01995"
     CDS             2278..>2913
                     /locus_tag="PLY30_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64905.1"
                     /translation="MNQAAFAAPSAGIEIAVPQEKPGFIQIDIPADLASLDDIFEAPP
                     STDPRLVLHIQNAHANYDAQTRIKGLLEYLSSQYGIGTIFVEGAADTLNPEYLKFFPD
                     AERNRMLADRLARKGELTGAELFVLEADRQKIRAEGIESAGLYRKNYEALKAVYENET
                     VINRFVDGLESRLSMIASKIAGGDMRRVISDWKKFENGYREFMPYVRSLAAD"
BASE COUNT          664 a          782 c          817 g          640 t
ORIGIN      
        1 ggcggcgggg gagatgcgcg ctcgcgtcga gaggctcacg aaccagcagg tctggatcag
       61 cacgttccac tcgacctgtt tgcgcatcct tcgcatggac ggcgccgcga tcggtctgga
      121 tccccatttc cttgtttatg acgaatccga ccagctccag ctgataaagg agtgcatccg
      181 cgagctcagt ttcgacgaga aaaaggtcca tcccaaggga gtgaaggagg agatccagcg
      241 cgccaaggat tatctccgga cgccctgtca gttcgcggaa aaggcgatgg atgtctacca
      301 ggacgcggtc gcgaaggtgt acatgctcta cgaagagaaa atgcgccgcc tcaaggcgtg
      361 cgatttcggc gatttgatta tgaagacggt ggcgctctat gaccgcgccc ccgaagtcct
      421 gaaggcgtgg cagagccggt tccggtatat cctgatcgac gaataccagg acacgaatca
      481 tgcccagtat cgctttgccg ggttgctggc cgccgcccgc aaacagatca cggtcgtcgg
      541 ggacccggac cagtcgatct atgcctggcg gggagccgac atcaggaata tcctcaactt
      601 cgaagcggat tatccggagt gcggcgtgat ccggatggag cagaactacc gttccacgtc
      661 cgtgatcctg gacgcggcga accatctcat ccgcaacaac cagatgcgaa aacccaaaga
      721 cctctggact gaacgaaagg gtggcgaacc gattgaactc tatgaagcgg aagacgaggt
      781 cgacgaggca caatatgtcg tgtcgaacat cctgcgccac aggtcggcgg ggcgaaaact
      841 cgcagaccag gtggtctttt accgcgtcca tgcccagtcc cgcgtgatcg aagaccatct
      901 gcggcgcgcc aatatcccgt acaagatcgt cggcggcgtc cggttctacg atcgcaagga
      961 gatcagggat gtgatcgcct atcttcgcct ggtcgcgtat ccgacggacg aactcagtct
     1021 caagcgggtg atcaacgttc cggcccgcgg catcggaaag aaggcgatgg agacacttga
     1081 ggcggaggtt tcccgggccg cgggagatgt ttcttcgtgg gattccgtgc tcgcggatgt
     1141 cgcgaagatc ggggggctgg cgccccgcgt caaaaagaat ctgacggagt tctacgggat
     1201 gattaaacgg cttcgcacgg aaagccggga gcttcccgtc cgggatattc tcgaacgcgt
     1261 gctcgaagaa tcacgctaca tcgcggaact tgaagccgaa cacacagccg aggcggaagc
     1321 gagggtcggg aatatcgagg agttcttcag cgtgatcgac gattttgttc aaagcggagg
     1381 aggaaacggt gccgggattt tcggactgac gggtgatggc cctcaggcgg cggtatccgg
     1441 tccgcggttg tcgcccctgg agctgtttct cgagtcgatt gagcttgtca ccgatctcga
     1501 tcaatgggac caggggacga acgtcctgac attgatgacg cttcataccg ccaaaggcct
     1561 cgaatttccc gtcgttcaca tggtggggct cgaagaggga gtgtttccgc atgtgaactc
     1621 ctatctcggg tccgcggatg aaatcgagga ggagcgcagg ctttgctatg tcgggattac
     1681 tcgtgccagg gagaaactcc acatttcata cgcttcggcg cgccgtctct atggcgcgat
     1741 ccatcacaat ttaccgtccc ggttccttac cgaaatcccg ccccatctcc tgcgggccgt
     1801 ccggggttcc ggatcgtctt ctgagtatcg ggatgatgac cgggatgagt ttcccgattc
     1861 ccgtatcgag gacgatcccg tcatcgattt tgatttctag ccttcccatt cgatacctct
     1921 gttccattat cagtagcgaa aatattttca tgtttaattt ttatttaacg taacccgttt
     1981 aaaatcaatg tcttggatta tattaaataa tattacaatc atattgacag taacacattc
     2041 ataggttaac tttctattag atcaacacgc aaaagataac aaaaactaaa aatgaaattt
     2101 ataagaattt ctccatattc gttttgttca ggacttgtat tatccgattt gcggtgttac
     2161 ctttgcaaag tattatacaa aagacaaaaa atgtctttag cttgagagga gatagccgat
     2221 nnnnnnnnnn cgcgattctg gcgcgtcaca agttgtctga cggtcctgtg cttcattttg
     2281 aatcaggctg ccttcgccgc tccgtcggcg gggatcgaga ttgccgttcc gcaggaaaaa
     2341 cccggattca ttcaaatcga tattccggcc gacctcgcga gccttgacga tatctttgaa
     2401 gcgcctcctt cgacagatcc ccggctcgta ctccacatcc agaacgcgca cgcgaactat
     2461 gacgcgcaga ccaggatcaa ggggctcctt gaatatctga gctcgcaata cgggatcgga
     2521 accatcttcg tggaaggcgc cgccgatacg ctcaatcccg aatatctgaa attcttcccc
     2581 gatgcggagc gtaaccgcat gcttgcggac cgtcttgccc gaaaagggga actgacgggc
     2641 gcggaacttt ttgtccttga ggcggaccgc cagaagatcc gggccgaggg tatcgagtcc
     2701 gccggccttt accggaagaa ttacgaagcc ctcaaggctg tgtatgagaa cgagaccgtc
     2761 atcaaccggt tcgtcgatgg tctcgaatcc cgcctttcga tgatcgcaag caagatcgcc
     2821 ggcggcgaca tgcgccgcgt catctccgac tggaaaaagt tcgagaatgg ttaccgcgag
     2881 ttcatgccgt atgtgcgttc cctggccgct gac
//
LOCUS       DAOOBX010000096         2888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_38246_length_2888_cov_3.310978, whole genome shotgun sequence.
ACCESSION   DAOOBX010000096 DAOOBX010000000
VERSION     DAOOBX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38246_length_2888_cov_3.310978"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PLY30_02000"
     CDS             <1..791
                     /locus_tag="PLY30_02000"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPW64906.1"
                     /translation="AERFGVAEGGVTVMIHSGSRGFGYQVCEDSLAAARRALSKYGIQ
                     VPDIQLACAPVHSGEGKQYLAAMRCAANFAWANRQCLAHLARQVFERFFERGPRDLGM
                     DLIYDVAHNIAKFERFTVDGKERLLCVHRKGATRSFAPGHDELPEDYRDTGQPVIIPG
                     DMGRNSCLLAGTRRAMEETFGSCCHGAGRALSRTAALNAARGHSIRRELEERGIIVMA
                     RGEKGIAEEQPAAYKDVNEVVETVSGAGLARKVARMRPIGVIKG"
     gene            1093..1734
                     /locus_tag="PLY30_02005"
     CDS             1093..1734
                     /locus_tag="PLY30_02005"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HPW64907.1"
                     /translation="MKNYTDLGLKTYLEKLSSDEPVPGGGSVSAYVASLGMGLTQMVA
                     RIAMKRKPKAGATPEQKFQAEANKKRMEEIVSSLEKTKEQSLQIVSLDPKVYDEVMDC
                     YRSGAAPEKLEDALENAFRLQADLALIIVMAKEWNAHMKEIVKGAVANDLIVSDQLLG
                     AAFEGAYHTAYINTVYMKNPERKSRADNALMELRNRYEARGEKGPPPVSVNPA"
     gene            1754..2611
                     /locus_tag="PLY30_02010"
     CDS             1754..2611
                     /locus_tag="PLY30_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPW64908.1"
                     /translation="MDARRLEGKTIAADIQERIAGKVQTFADRGLPKPSMVSIQTSED
                     PSAAWYIGQQEKLAAKLGIGFRRVPSSEIKDERTLLEKIAAFNGEPGVHGLFVSMPLP
                     QGFDADKVLLAMDPRKDVEGIHPTSLGLIVLRKGKLIPPTAYAAFALIRSTGVMLHGK
                     KAALVGQSAIVGRPLQLLLGENRVTTLVCNTGTSEDDIKKIVGGSDIVVACAGKAGMI
                     KGDWIKKGAIVIDVGTTEVGGKLTGDVEYEAASKKAGFITPVPGGVGPLTVTMLMENL
                     MHAYEWQKK"
     gene            2684..>2888
                     /locus_tag="PLY30_02015"
     CDS             2684..>2888
                     /locus_tag="PLY30_02015"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPW64909.1"
                     /translation="MKLTPDNIYGKKARGEKIAALAAYDFPTARILDGAGIDILMVGD
                     SLGMVLLGYESTRQVTMRDMAHHT"
BASE COUNT          628 a          800 c          954 g          506 t
ORIGIN      
        1 cggcggaacg gttcggcgtc gcggaaggcg gggttacggt catgatccat tcgggctcgc
       61 gcgggttcgg ctaccaggtc tgtgaggatt ctcttgcggc cgcccgccgg gcgctttcaa
      121 agtacggtat ccaggtgccg gatatccagc tcgcctgcgc gccggtccat tccggggaag
      181 gaaagcagta cctcgcggcg atgcgctgtg cggcgaattt tgcctgggcg aaccggcagt
      241 gtctcgcgca tctggcgcgg caggtgttcg aaagattctt cgagaggggt ccccgggacc
      301 tcggcatgga cctcatctac gatgtcgcgc ataacatcgc gaagttcgaa cggttcacgg
      361 tcgacgggaa agaaaggctt ttgtgcgttc accgcaaggg cgcgacgcgg tcgttcgcgc
      421 cggggcacga cgaacttccg gaagactacc gtgataccgg acagcctgtg atcatccccg
      481 gggatatggg gcgcaattcc tgcctgctgg cagggacccg ccgggccatg gaggagacct
      541 tcgggtcctg ctgccacggc gccggacgcg cgctgtcgcg aacggccgcg ctgaatgccg
      601 cgcggggcca ctcgatccgc cgggagctcg aggaacgcgg catcattgtg atggcccggg
      661 gcgaaaaggg gatcgcggag gagcagcccg ccgcctacaa ggacgtcaac gaggtggttg
      721 agacagtttc gggcgccgga cttgcccgga aggtcgcgcg gatgcgtccc atcggcgtta
      781 tcaagggcta gagggaccgg cgaattccgt ttttctgaag ctttccaaac tgtagtataa
      841 tgaatcggca agaggaacga atggatctct ccgcttgcat aggtgatggc ctcaaataat
      901 ccggagtgtg aaggctggca ggaagttcgt gatgatcccg acggatgacg atcaaaaaaa
      961 acgcttatcg cgcccgggaa ataacgcgcg gttcgactca cggcgcccgc ttgtccgcaa
     1021 gacaggcgca aactagaaaa cattttacgt tgtgcgttgt tcgttgcgcg taccggtaaa
     1081 agagtctttc tcatgaaaaa ctacacggat ctcggtttaa agacttacct ggagaaatta
     1141 tcaagcgacg agcccgtccc gggcggcggg agcgtttccg cctatgtggc ctcgctcggc
     1201 atggggctga cgcagatggt cgccaggatc gcgatgaagc ggaaacccaa ggcaggcgcg
     1261 acaccggagc agaaattcca ggccgaggcg aacaagaaac ggatggagga gatcgtcagc
     1321 tctctcgaaa agacgaagga acagtcgctg cagatcgttt cgctcgatcc caaggtctat
     1381 gacgaggtga tggattgtta ccggtccggg gccgcgcccg aaaagctcga ggatgccctc
     1441 gagaacgcgt tccgcctgca ggccgatctc gccctgatca tcgtgatggc gaaggaatgg
     1501 aacgcccaca tgaaagagat cgtcaagggg gcggttgcca acgaccttat cgtgagcgat
     1561 cagcttcttg gcgcggcctt cgagggcgcc tatcacaccg cgtatatcaa cacggtttac
     1621 atgaagaatc ccgaacgcaa gagccgcgcc gacaacgcgc tcatggaact gagaaaccgg
     1681 tacgaggcca ggggcgaaaa aggaccgccg ccggtttcgg tcaaccccgc ctgaaacaat
     1741 aaaggagcct gaagtggacg ccaggagatt ggaagggaaa acgatcgccg ccgatatcca
     1801 ggagaggatc gccgggaagg tccagacctt cgccgaccgg ggactcccga agccatccat
     1861 ggtctcgatc cagacgagcg aggacccgtc ggccgcgtgg tacatcggac agcaggaaaa
     1921 actcgcggcg aagctcggga tcggtttccg gcgggttccg tcctccgaga tcaaggacga
     1981 gcggacgctc ctggaaaaga tcgccgcgtt caacggggag cccggcgtgc acggtctttt
     2041 cgtttcgatg ccgctcccgc aagggttcga cgcggacaag gtcctgctcg cgatggatcc
     2101 gcgaaaggac gtcgaaggca tccacccgac gagcctcgga ctgatcgtgc tccgcaaggg
     2161 gaaattgatc ccgccgaccg cgtacgccgc ttttgcgctg atcaggtcga cgggcgtgat
     2221 gcttcacgga aagaaggcgg cgctcgtggg tcagagcgcg atcgtgggac gccctctcca
     2281 gctgcttctc ggcgagaacc gggtgacgac ccttgtctgc aataccggga cctcggaaga
     2341 tgacataaaa aagatcgtcg gcggttcgga tatcgtggtc gcttgcgcgg ggaaggccgg
     2401 catgatcaag ggggactgga tcaagaaagg cgcgatcgtg atcgatgtcg ggacgaccga
     2461 ggttggcgga aaactgaccg gcgacgtgga gtatgaggcc gcgtcgaaaa aggccgggtt
     2521 catcacgcct gtccccggcg gggtcggccc tctgaccgtc acgatgttga tggaaaacct
     2581 gatgcatgcg tatgaatggc aaaagaaatg atccaggtgc ggagtgcggg gggcgggata
     2641 aaagtttttg cttcgcaccc gggtctccgt gcccagtaca catatgaaac ttactcctga
     2701 caatatctac ggcaagaaag cgagaggcga gaagatagcg gccctggccg cctacgattt
     2761 tccgaccgcg cggatcctgg acggcgcggg gatcgatatc ctgatggtgg gagactcgct
     2821 cggcatggtg cttctcggct acgaaagcac gcgtcaggtc acgatgcggg atatggccca
     2881 tcacacgc
//
LOCUS       DAOOBX010000097         2878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_38436_length_2878_cov_3.155863, whole genome shotgun sequence.
ACCESSION   DAOOBX010000097 DAOOBX010000000
VERSION     DAOOBX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38436_length_2878_cov_3.155863"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2878)
                     /locus_tag="PLY30_02020"
     CDS             complement(<1..>2878)
                     /locus_tag="PLY30_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64910.1"
                     /translation="GSRLFDELNRFLPRVARSLARTSADLRTADHYAKLFILEDLASL
                     ELTHQTLSEYQKDPESYLGLVNDPDLRSRFTPAIEFYQTALRRDRVFYEKVRAVKNAR
                     SIVIIAGGFHTNGLERILKENGESYVVVTPKIASLAGAGNYFTVMAGDLSYKDYLKTT
                     YYDALMRHAVRSLTATLKEPEFRQTIKRWRDNLIRALAAKGRIDDLGRYTRYLDDLNL
                     VYAERFAKNLTPRTKEDILDFIRKEFVKFRAEAFDSLQRRFEGQLSKFTADIQNLTGR
                     NQLTEASVEAVLRESGLTGRSMLGAPSAMAPENPVLGDPGIAGPEGSTIPWRNEAGAA
                     DGRDLSRLAGMGGFYIDGISSAGNVLDAANALKAAANSDRSFHEKTTAPSEVLVTLIP
                     NGMKLIIAPRHVIDPRNVPGEPGKKHYMSHEEFSEAAGFEEQPAETVRMLLFFDHWTG
                     NFSEMLLVPGVGQRREDVLPLFSFLRNALIKTGNPAEAVDGSLMMDPFGDGEITLGDF
                     IGRQELRSPFPRIIQKILMAGTVAGLLAIPAITAAAAPAISPERVKKARAVFKTGKGQ
                     VEARAGVKPAARRSETRSLASILASVLPEETVALTVWDYDTGAGDFIRAIYEWLRDQG
                     YKNEIDVFGTERDETVVKPAFRDRIIRYSAEDPVGLPGRRPGSVRVIFANNLVARESG
                     DLEAGPLAREAMKYLDPESGVLLVTIEASDAAAGRDEKVLYDLEKAGFKVLMQDVPAD
                     YPDYETVFKHSKLIVAIPPRAETRAEDKDRAAFREVGGDREWKSSVGSSAIRIRPQNG
                     VVIETPSNKNKYRFLLKDDRGLRIGVQRFELTPSGELIEGSIHWRELGPVYIGHFYGR
                     LGDRNSFGVGDGFLLAPVHCQIDFGKDDKGFYLTISDLSYEFTSIEGQTTGLTVKWTP
                     QPGSDSVEELRVESTSADGDSDDGGSDDDDSGWLVDETPGTQDAY"
BASE COUNT          595 a          892 c          761 g          630 t
ORIGIN      
        1 tatacgcatc ctgcgtgccg ggggtctcat cgaccagcca acccgagtca tcgtcatccg
       61 agccaccgtc atccgagtca ccgtcggcag acgtcgattc aacacgcagc tcctcgacag
      121 aatcgctgcc gggctgcggc gtccacttga cggtcagacc cgttgtttgg ccctcgatgc
      181 tcgtgaactc atagctcaaa tccgaaatcg tcaggtagaa gcctttatcg tccttgccaa
      241 aatcaatctg gcaatgcacc ggcgcgagca aaaaaccatc cccaacacca aaactattac
      301 ggtcgcccag acgcccgtag aaatgaccga tatatacggg tcccagttct ctccaatgaa
      361 tcgaaccctc tatcagctcg cccgaaggcg ttagctcgaa ccgctgaacc ccgatgcgca
      421 gaccacgatc atctttcaaa agaaaccggt atttgttctt gttgcttggt gtttcgatca
      481 cgacaccgtt ttgggggcgg atcctgatcg cggaacttcc caccgaagat ttccattccc
      541 gatcgcctcc gacttcccgg aaggccgccc tgtccttgtc ttcagcgcgc gtttcggcgc
      601 gcggagggat cgcaacgata agtttggaat gtttgaaaac ggtctcatag tccggatagt
      661 ccgcgggaac atcctgcatc aagaccttaa accccgcttt ttcgagatcg tacaggacct
      721 tttcatcgcg gcccgcggcc gcgtcgctcg cctcgatcgt gacgaggagg acgccgcttt
      781 cgggatcaag atacttcatc gcttcccggg caagcggtcc ggcttccagg tcccccgatt
      841 cgcgcgcgac gaggttgttc gcgaagatca cccgcacgga tccggggcgt ctgccgggaa
      901 gcccgaccgg atcctcggcc gaataacgga tgatccggtc ccggaaggcc ggtttgacga
      961 ccgtttcgtc ccgttccgtc ccgaacacgt cgatctcatt tttatacccc tgatcccgca
     1021 gccactcata tatcgcacgg atgaaatccc ccgcgcctgt gtcgtaatcc cagaccgtga
     1081 gcgcgaccgt ctcctcgggc aaaacactcg cgaggatact cgcaagactc cgtgtttccg
     1141 agcgccgcgc agcaggcttt acacccgcgc gtgcttcgac ctgccctttg ccggttttaa
     1201 atactgctct tgccttcttg actctttccg gagagatggc tggagcggcg gcggccgtta
     1261 ttgctggtat agccagcagc ccggcaacgg ttccggccat gagtattttt tgaatgatcc
     1321 gcgggaacgg actccgaagt tcctgccggc cgatgaaatc acccaaagtg atctcgccgt
     1381 cgccgaaggg atccatcatc agggatccat ccaccgcctc cgcgggattt cccgtcttga
     1441 tgagggcatt ccgcaggaat gaaaacaggg gcaggacatc ttcccgccgc tggccaacgc
     1501 ccgggactaa cagcatttca ctgaaattgc cggtccaatg gtcaaagaag agcagcattc
     1561 tgactgtttc cgccggctgc tcctcaaaac ccgcagcctc cgagaattcc tcatgcgaca
     1621 tgtagtgttt tttgccgggt tcgccgggca cgttccgggg atcaatcaca tgtctggggg
     1681 caataatgag cttcattccg ttcggaatga gggttaccag cacctccgac ggggcggtcg
     1741 tcttctcgtg gaatgacctg tccgaattcg cagcagcttt cagagcattc gcggcatcaa
     1801 gaacattccc ggcagacgag atgccgtcaa tataaaatcc tcccatcccg gcaagccggc
     1861 ttaaatcgcg accgtccgcc gctccggctt cattccgcca agggattgtt gagccttcgg
     1921 gcccggcaat gcccggatcc cccaggacgg gattttccgg cgccatcgca ctcggggcgc
     1981 cgagcattga acggcccgtg aggccggact cgcggagaac cgcctcaacg ctcgcttccg
     2041 tcagctggtt cctgcctgtg agattctgaa tgtccgcggt gaattttgaa agctgacctt
     2101 cgaaccgtcg ctgcaatgaa tcgaacgctt ccgccctgaa tttcacgaac tcttttctta
     2161 tgaaatcaag gatatcctct ttcgtccggg gcgtcagatt tttggcaaat cgttcggcat
     2221 acacgagatt caggtcatcg aggtagcggg tgtaccgtcc gaggtcatcg atccggccct
     2281 tcgcggcaag agcgcggatc agattgtccc tccaccgctt gatcgtctgg cggaactccg
     2341 gctcttttaa cgtcgccgtc aggctcctca cggcatgccg catgagagcg tcgtaatagg
     2401 tcgtctttaa gtaatcttta taggacaggt cgcccgccat caccgtgaag taattcccag
     2461 cgcccgcaag cgatgcgatc ttcggcgtca ccaccacgta ggattcgccg ttttccttca
     2521 gaatacgctc aagaccgttc gtgtgaaaac ctccggctat gatgacaatg gaccgggcgt
     2581 tcttgacggc ccggaccttt tcgtagaaca cccggtcacg acggagcgcg gtttgataga
     2641 actcaatcgc gggcgtaaag cgggaccgaa gatccggatc attcacgagc ccgagataac
     2701 tctcgggatc tttctggtat tcggaaaggg tctgatgcgt cagctcaaga ctcgcgagat
     2761 cctcaagaat gaaaagtttc gcgtaatgat ccgccgtcct gagatcagcc gatgtgcgcg
     2821 cgaggctccg cgccactcgg ggcaggaacc ggttgagttc atcaaaaagt ctgctccc
//
LOCUS       DAOOBX010000098         2876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_38467_length_2876_cov_2.912088, whole genome shotgun sequence.
ACCESSION   DAOOBX010000098 DAOOBX010000000
VERSION     DAOOBX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38467_length_2876_cov_2.912088"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..405)
                     /locus_tag="PLY30_02025"
     CDS             complement(91..405)
                     /locus_tag="PLY30_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW64911.1"
                     /translation="MNNMLTLDEVKNYLEIEQQEIERLIKNDLLHAYKIGGVYLRFRK
                     EEVLNLKYDVLLKKKQQGPKVSVGHRLVDFWRFNNFYVLSLIVIVALLYWFLRSSPAV
                     SL"
     gene            complement(452..982)
                     /locus_tag="PLY30_02030"
     CDS             complement(452..982)
                     /locus_tag="PLY30_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPW64912.1"
                     /translation="MAKKGNELQEKILDVDASMQGTITFKDPVNLRINGSFEGKLDTR
                     GNLTIGENAKVTANINGDSIVVAGKVSGDIVATKSISVMGPATIRGNIRTPRLSVADG
                     AILDGQLTMSGAGSFEGSDVLLTLKDVAQYLKVESRVIEEWAESHKIPARFENGNWVF
                     SKSEVDQCIQQEKVRV"
     gene            complement(1166..2251)
                     /gene="mnmA"
                     /locus_tag="PLY30_02035"
     CDS             complement(1166..2251)
                     /gene="mnmA"
                     /locus_tag="PLY30_02035"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPW64913.1"
                     /translation="MASRSSRKIVVGISGGVDSSVSLMLLKKAGWDPVGVSLKYPVWK
                     DPSNGLRENVCCSAESFRIAKDICRKLDVPHHLCDVSREFKKEVIGYFLNGLRNARTP
                     NPCVMCNRFLKFKKLFAWAGRRGIRHVATGHYARVRRDSRTGLCRLVTARDREKDQTY
                     SLSFLSQAQLRNILLPLGDMTKREVYDTAVREGFEVFLKRKQSQDFCFVAGNSMGAFL
                     AREVGVKEGEMRDDDDAVAGHHRGLHFYTIGQRKGLGLSGGPFFVKGFEPAKNVLRVT
                     KDERALLQKEVRLGPCNFISGIPGRIIRVRAKIRYRQDAAPARLIVDAGKRATLVFDK
                     PQRAVTPGQFAVFYQRDVCLGAGEIQG"
     gene            complement(2254..>2876)
                     /locus_tag="PLY30_02040"
     CDS             complement(2254..>2876)
                     /locus_tag="PLY30_02040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPW64914.1"
                     /translation="KPRLFRCQMERIRSWGYRGITIAECARRKWVAGFFEKAVALTFD
                     DGYLDNYQAAFPVLCELKLPGTFFVTPGMVGEKGFMTWGMLTDMSRVSGIEIGSHGLF
                     HKPLADLPESEARRSVFDSKKAIEDRLGRPVKAFSYPSGSFNDRVVELVREAGYEYAC
                     AASHVHDRRYVGNPFLIRRIKISSSSESPVAFAWRLSGYYHRFGRP"
BASE COUNT          623 a          893 c          763 g          597 t
ORIGIN      
        1 aagcagccgc gccccctgct tcacgatggc cgcctccacc ctcgcgatct tgacgagatc
       61 aactccctgc aggaccttca ttcccgcgcc ctaaagggag accgccggcg aggaccggag
      121 gaaccaataa agcagcgcaa caatcactat cagtgaaaga acgtaaaaat tgttgaaccg
      181 ccagaaatcg acaagccggt gaccgaccga gaccttgggc ccctgctgtt ttttcttgag
      241 gaggacatcg tacttcaagt tgaggacctc ttccttgcgg aaacggagat agaccccgcc
      301 gatcttgtag gcgtggagca ggtcattctt gatcaagcgc tcgatctcct gttgctcaat
      361 ctccaggtaa ttcttgacct cgtcgagggt aagcatgttg ttcatggaac gaccttacgg
      421 attgtgaaaa tgcggaaaac ggaacccggc gtcagacgcg caccttttcc tgctggatac
      481 actgatcgac ctcggacttc gagaacaccc agttaccgtt ctcgaaacgc gccgggatct
      541 tgtgtgactc ggcccactcc tcgatcaccc tggattccac tttaaggtac tgggcaacat
      601 ctttcagggt caaaagaacg tcggaacctt cgaaggaacc ggcgcccgac atggtgagct
      661 ggccgtccag gatcgcgccg tccgcaacgc tcaaacgcgg cgtacgaatg ttcccgcgga
      721 tcgtggcggg ccccatcacg gaaatgcttt tcgtcgcgac gatatctccg gagactttgc
      781 cggcgaccac gatgctgtcg ccgttgatat tggccgtcac cttggcgttc tcgccgatcg
      841 tcaggttgcc gcgggtatca agcttgcctt cgaaactgcc attaatgcgg aggttaacgg
      901 ggtccttgaa cgtaatggtc ccctgcatgc tcgcatcgac atcgaggatc ttttcctgaa
      961 gttcgttccc ttttttcgcc atggcttcgg cctccttcgt tcccgtccgg acggacggtt
     1021 cggtgtgatg atgagggatt atgtccctta atcaattgct tgtcaatgaa tggcacatgc
     1081 cgccacacgc acactgacac acgccacacg aaaacctttt caccgggggt tctccttgcg
     1141 tccttgcggt gtgcggcttc gcgacctagc cttgtatttc ccccgcaccg agacatacgt
     1201 cccgctggta aaagaccgcg aattgtcccg gcgtcacggc ccgctgaggc ttatcgaaaa
     1261 cgagagtcgc ccgttttccg gcgtcgacga tcagccgggc cggcgcggca tcctggcgat
     1321 aacggatctt ggcacgtacc cggattatcc gtccgggaat gccgctgata aaattgcacg
     1381 gtccgagacg cacctctttt tgaagaagcg ctctctcgtc cttcgtgacc cggaggacgt
     1441 tcttcgccgg ctcgaatccc ttgacaaaaa aaggccctcc cgagagtcca agcccctttc
     1501 tctggccgat cgtataaaag tgaagccccc ggtgatgacc ggcgaccgcg tcatcatcat
     1561 cccgcatctc cccttctttc acgccgactt ccctggcaag aaaagccccc atggaattgc
     1621 cggcgacaaa acagaaatcc tggctctgtt tgcgtttgag aaacacctcg aatccctccc
     1681 tcacggccgt atcataaact tcccgcttgg tcatgtcccc aagcggcagc aggatattcc
     1741 ggagctgcgc ctggctgagg aaactgaggg aataggtctg gtctttttcc cgatcccggg
     1801 cggtcacaag ccggcaaagt ccggtccggg aatcacgacg gacgcgcgcg taatgcccgg
     1861 tcgccacatg ccggatcccg cggcggccgg cccatgcgaa gagctttttg aatttcagga
     1921 accggttgca catcacacac gggttcggcg tgcgcgcatt ccggagcccg ttgagaaaat
     1981 aaccgatcac ttctttttta aattcccgcg aaacgtcaca gagatggtgg ggcacgtcga
     2041 gcttccggca gatgtctttt gcgatgcgga aggattcggc actgcagcag acgttctcgc
     2101 gcaggccatt cgagggatct ttccagaccg gatatttgag ggagacgccg accggatccc
     2161 atcccgcctt tttgaggagc atgagagaca cggaggaatc cacacctccc gaaattccca
     2221 cgacgatctt tcgcgacgaa cgggacgcca cggtcaggga cgcccaaagc ggtgatagta
     2281 gcccgaaagc ctccacgcga aagcgaccgg ggattccgag gacgaagaaa tcttgatcct
     2341 gcgtatcaga aaagggttcc cgacatacct gcggtcatgc acgtggctgg ccgcgcacgc
     2401 gtattcatag cccgcttcgc gcacaagttc cacaacccgg tcattgaacg atcccgacgg
     2461 gtacgagaac gccttgacgg gcctgccgag cctgtcctcg atggctttct tggaatcaaa
     2521 aaccgaacgg cgcgcctcac tctcgggaag atcggcgagc ggtttatgga aaagcccgtg
     2581 gcttccgatc tcgattccgg aaacccggct catgtccgtg agcatccccc acgtcatgaa
     2641 ccccttctcg cccaccatcc cgggcgtcac aaagaacgtc cccggcagtt tcaattcgca
     2701 aagcacggga aacgcggcct gataattgtc gagataaccg tcatcaaagg tcaacgcgac
     2761 cgccttctcg aaaaaacccg cgacccattt ccgccgcgcg cattcggcga tcgtgatccc
     2821 ccggtacccc cacgagcgga ttcgttccat ctgacaacga aaaagccggg gtttga
//
LOCUS       DAOOBX010000099         2854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_38844_length_2854_cov_3.236156, whole genome shotgun sequence.
ACCESSION   DAOOBX010000099 DAOOBX010000000
VERSION     DAOOBX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38844_length_2854_cov_3.236156"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PLY30_02045"
     CDS             <1..1048
                     /locus_tag="PLY30_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64915.1"
                     /translation="PEEAENRDNESVPYACRIFDLIEAPDKAGHFFIGTEHGVFETID
                     GAHNWHPLSQSGLQSTRVVSLAYAGKTDALFAATPHGIYRYRPAKEVWEALSEGLARS
                     GAKSLAITGNSRLIAITGDGFMEYPIAPETAPPALLYIPSPETLEMFQTLVRLEPSAR
                     DIQKAVIRFANVANGKIKRWQIASRAAGLLPSLSIGKSRDYDASISTYSGKFITGPDD
                     VSKSWDTDVRWDLGDILYSSDQTSIDSREKMMVELRQDLLSEATRIYYERRRLQVDLV
                     YTPSLSGQEHLERLIRLDELAALLDGMTDGFLSKQLEKIYARNAELNKLWEYGQHEMR
                     GTAHGEIQTQGKEK"
     gene            1050..1562
                     /locus_tag="PLY30_02050"
     CDS             1050..1562
                     /locus_tag="PLY30_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPW64916.1"
                     /translation="MQNRNQNQNGYRKNSWSKENRDRPETFLRDAPPQDITESRPVTA
                     ERVVESTTRSRIKSFKELIVWQKGMALAKEIYAATTTFPREELYGLVSQLRRSAVSVP
                     SGIAEGFRRRFQKELKPFLDIALGSLAELETQVAIAGELGYLKPETLKRLGDMTDQVI
                     RMTIGLSKKT"
     gene            complement(1559..2590)
                     /gene="rlmN"
                     /locus_tag="PLY30_02055"
     CDS             complement(1559..2590)
                     /gene="rlmN"
                     /locus_tag="PLY30_02055"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPW64917.1"
                     /translation="MKALYGSTASEMEALIVSSGFPKFRAAQILDWLYRKHVLAWEEM
                     KNLPKDLLKAIAGSHRLTALQMEEAGVYAGEGSSKFLFRTADGHLLESMLISQENRET
                     VCVSSQLGCPVQCPFCASGKGGFIRNLTAGEIIEQIIWIARQSGRKITNVVFMGMGEP
                     LENFDEVMKALEILTAQWGFEMGGRKITLSTSGVTKRIYDFVKCTDGRIRLSVSLHSA
                     DDHARSRLIPYNRKYPLSGLIAELKKVHRVLKRDITFEYTLIDGVNDSEADAKAVARI
                     AKPLQAKINLIPYNPIREVVWKTPSRERMDRFRETLEKSGVRSTFRKSAGKDVDGACG
                     QLRLDRVRS"
     gene            complement(2728..>2854)
                     /locus_tag="PLY30_02060"
     CDS             complement(2728..>2854)
                     /locus_tag="PLY30_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HPW64918.1"
                     /translation="YGMNFKHFPELDWPWGYPFALGLCLLSSLAMLVWVKIKKWI"
BASE COUNT          694 a          824 c          776 g          560 t
ORIGIN      
        1 tcccgaagaa gcggaaaaca gggacaacga atccgtgcct tatgcctgtc gcatctttga
       61 cctgatcgag gcgcccgata aagcgggaca tttttttatc ggcacggaac acggcgtttt
      121 cgaaaccatc gacggcgcgc acaactggca tcctctctcc caaagcgggc ttcagagcac
      181 gcgggtcgta agccttgcat acgccggaaa aacagacgct ctcttcgccg ccacaccgca
      241 cggcatctac cgctaccggc cggcaaaaga agtgtgggag gcgctttccg aagggcttgc
      301 gagatccggg gccaagagcc ttgcgattac cggcaattcc agactgatcg cgatcacggg
      361 agacgggttt atggaatacc ccatcgcccc cgaaacggcc ccgccggccc tgctgtatat
      421 cccctcgcct gaaacgctcg aaatgttcca aacccttgtc agactggaac cttcggcgcg
      481 tgacatccag aaggccgtta tccgtttcgc gaacgtcgcg aacggcaaga tcaagcgatg
      541 gcaaatcgca tcgcgagccg cggggctgct tccgtcgctc tcgatcggca aaagccgcga
      601 ttacgacgct tccatcagca cctacagcgg caaattcatc acagggcccg acgatgtcag
      661 caagagctgg gacacggacg tcagatggga ccttggcgac atcctttaca gctccgatca
      721 gacctcgatc gattcccgcg aaaagatgat ggtcgagctg cggcaagacc ttctttcgga
      781 ggcaacccga atctattacg aacgccgtcg gctccaggtc gaccttgtct acaccccctc
      841 cctttccgga caggaacacc tcgaacgact gattcgcctg gacgagctcg ccgcactcct
      901 cgacggcatg accgacggtt tcctgtcgaa acagctggag aaaatctacg caaggaatgc
      961 agagctgaac aaattatggg aatacggtca gcacgaaatg cggggtacgg cacacggaga
     1021 aatccaaact caaggaaagg agaaataata tgcaaaacag aaatcagaac cagaatggct
     1081 acagaaaaaa ttcatggtca aaagaaaacc gggatcgccc tgaaacattt ctccgggatg
     1141 ccccgccaca agacatcaca gagtcccgtc cggtaaccgc cgagcgggtc gtcgagtcaa
     1201 cgacccgctc caggataaag agtttcaagg aattaatcgt ctggcaaaag ggcatggcgc
     1261 tggccaaaga gatctacgcg gcaacgacaa cctttccgcg ggaagagctg tacggtttag
     1321 tgtcccaatt aaggagaagc gctgtttcgg taccctcggg tatcgcagag ggctttcgga
     1381 gaaggtttca aaaagagcta aaaccgtttt tagacattgc gctcggatcc ttggccgagc
     1441 tcgaaaccca ggttgccatc gccggagaac tgggctacct gaaaccggaa actttaaagc
     1501 gtttggggga tatgacagac caggtcataa gaatgacgat cggtttgagt aaaaaaacct
     1561 agctccgtac gcggtcaagc ctgagttgtc cgcacgcgcc gtccacgtct ttccccgcgc
     1621 tcttgcggaa cgtcgaccgg acaccggact tttcaagcgt ttcgcggaac cggtccatgc
     1681 gttcccggga gggagttttc cacacgacct cgcggatcgg gttgtagggg atgagattga
     1741 tcttggcctg gagcggcttc gcgatcctcg caaccgcctt cgcatccgct tccgaatcgt
     1801 tcactccgtc gatcagcgtg tattcgaacg tgatgtcgcg tttcagaacc cggtggacct
     1861 ttttcagctc cgcgatgagc cccgagaggg gatatttgcg gttgtaagga atgagccggc
     1921 ttcgcgcgtg gtcgtcggcg gaatgaagcg agactgagag ccggatccgg ccgtccgtgc
     1981 atttcacgaa atcatagatc cgcttcgtca ctcccgaggt cgacagcgtg atcttccgcc
     2041 cccccatctc gaaaccccat tgggccgtca gaatctcaag ggccttcatg acctcatcaa
     2101 agttctcgag cggttcgccc atgcccataa acacgacatt cgtgatcttc cggccgcttt
     2161 gccgcgcgat ccagatgatc tgctcgatga tctcgccggc cgtgaggtta cggatgaacc
     2221 cgcccttgcc gctcgcgcaa aaagggcatt ggaccgggca acccagctgg ctcgagacgc
     2281 acaccgtttc gcggttttcc tgcgaaatga gcatactttc cagcaaatgc ccgtccgcgg
     2341 tacggaagag gaacttcgaa gagccttcgc ccgcgtagac accggcctct tccatctgaa
     2401 gggccgtgag ccgatggctg ccggcaatcg ccttgaggag atccttcggg aggttcttca
     2461 tctcctccca ggcaaggaca tgcttacggt aaagccagtc gagaatctgg gccgcacgaa
     2521 attttgggaa accgctcgaa acgatcaggg cctccatctc ggacgcggta gaaccataga
     2581 gcgctttcat ggatcaaatc tcgaattttt aaatcacagg gtcgaaagga ttttgaatgt
     2641 ccgaacaccg cgggatcctt aagggaaagc gtgggcagga atccatgtcg gggttcagtt
     2701 ttagaggtcc ggattttccc gaaggatcta gatccacttc ttgatcttca cccagacaag
     2761 catggcaaga gaagagagca ggcacaaccc gagggcaaaa ggataccccc agggccagtc
     2821 cagctcggga aaatgcttaa agttcatgcc ataa
//
LOCUS       DAOOBX010000100         2847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_38985_length_2847_cov_2.327364, whole genome shotgun sequence.
ACCESSION   DAOOBX010000100 DAOOBX010000000
VERSION     DAOOBX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38985_length_2847_cov_2.327364"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLY30_02065"
     CDS             complement(<1..559)
                     /locus_tag="PLY30_02065"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HPW64919.1"
                     /translation="MDVTNTERIRKVAGLLRKAGSVLFITGAGISADSGIPTYRGIGG
                     LYNDRTTADGIPIETALAGETLRTRPGITWKYLAQIENRCRGAKHNRAHEILAGMEAR
                     FERVWVLTQNIDGLHQAAGSKNVIDLHGDMYVILCERCGWRTRVERYEGFAIPPRCPR
                     CGAAARPDVVFFGEWIPPRKVEMMER"
     gene            complement(550..864)
                     /locus_tag="PLY30_02070"
     CDS             complement(550..864)
                     /locus_tag="PLY30_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64920.1"
                     /translation="MSKRIAGVFIALFPTPAVHVIANGLHDDIAPAAAILIPGSKVKP
                     DGKAANRFKTRLDRGAELFCEGMRYMIAADGGRGPSGSREALVMADYFLKAPPEGSPL
                     WM"
     gene            982..2799
                     /locus_tag="PLY30_02075"
     CDS             982..2799
                     /locus_tag="PLY30_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPW64921.1"
                     /translation="MTPTQKRIELLAPAKDAAIGKAAVNCGADAVYLSAEKFGARERA
                     GNALVDIEALVRHAHRYYARVYIALNTILTDRELDEAFSLIKSLHAIGIDGLIIQDPG
                     LLELPLPPVPLFASTQMNNDSPEKVLFLEKTGFSRVILPREMTLNEIRAVRSKTSVDL
                     ECFIHGALCVSYSGQCYLSYAIGGRSANRGACAQPCRRLYSLVGADGKVFAGPKHLLS
                     LKDLNRSASLSELIDAGVSSFKIEGRLKDIAYVKNTVGFYRRELDAVLARKKRAKSSS
                     GTVIPGFVPDPAKTFNRGFTDFGLKGPTAGWSSPGTPKSLGERIGTVAKAAKDHFTLT
                     GPHDLANGDGICFFDARGELRGSVVNSVANDRVYPDKTGGLTAGAVIYRNHDRAFLKT
                     LTAKTSCERRIAVSFVLSGTPDGFRLTARDEDGNTADFSVPAAKQPAEKKAQAEETLV
                     KQISKLHDTAFTCISVTHTLPEICFLPVSLLNTLRRGAIETLIEARERARPRETAAIQ
                     PNDAPYPVKTLDYRANCLNRKAVDFFRRHGAEVTEMAAESGLPMQGRLLMRSKYCVKR
                     DLGSCGKMTEPLFLADENGRRFRLAFDCDGCRMLVFDDR"
BASE COUNT          543 a          953 c          845 g          506 t
ORIGIN      
        1 cgcgttccat catttcgacc ttgcgcggcg ggatccactc cccgaaaaaa acgacgtcgg
       61 gacgggcggc ggccccgcac ctggggcacc ggggcggaat cgcgaacccc tcgtagcgct
      121 cgacgcgcgt ccgccagccg caacgttcgc agaggatcac gtacatgtcc ccgtggaggt
      181 cgatcacgtt ctttgagccg gcggcctggt gaagaccgtc gatattctgg gtcaggaccc
      241 agacgcgctc gaaccgcgct tccatgcccg cgagaatctc atgggcgcgg ttgtgttttg
      301 cgccgcggca gcgattctcg atttgcgcga gatatttcca ggtaatcccg ggccgcgtcc
      361 gcaaggtctc gcccgcgagc gcggtctcga tcggaatgcc gtcggccgtc gtccggtcgt
      421 tataaagacc gccgattcct cggtaggtcg ggatccccga atccgccgaa attccggcgc
      481 ccgtaatgaa aagcacgctt ccggccttcc tcagcaatcc cgcgactttc cggatccgtt
      541 cggtgttcgt cacatccata atggcgatcc ctccggagga gccttgagaa agtagtccgc
      601 catgacaagc gcctcccggg aacccgatgg accccgcccg ccgtctgcag cgatcatgta
      661 gcgcatgccc tcgcaaaaga gttccgcgcc ccggtcgagc cgcgtcttga aacgattggc
      721 ggccttgcca tccggcttga ccttgcttcc ggggataagg atcgcggccg cgggtgcgat
      781 gtcgtcgtga agaccgtttg cgatcacatg aaccgccggc gtcgggaaca acgcgataaa
      841 aacccccgcg atccgtttgg acattctcat gccggtaatt atctcacaag cgcccggttt
      901 acggaaccct ccgccggagc gcgcaggcgg atttccggtg gcacggaacg cgcaagcccg
      961 tgtatagtat gcgtcacccc tatgacaccg acgcagaaaa ggatcgaact gctggccccg
     1021 gcaaaggacg ccgcgatcgg gaaggccgcc gtcaactgcg gcgcggacgc cgtctatctc
     1081 agcgccgaaa aattcggggc ccgcgagcgg gcgggcaatg ccctcgtcga tatcgaagcg
     1141 ctcgtccggc acgcccaccg ttattacgcg cgcgtctata tcgccctgaa cacgatcctg
     1201 accgaccgcg aactcgacga ggcgttttcc ctcatcaagt ccctgcacgc gatcgggatc
     1261 gacggcctta tcatccagga cccgggactt ctcgaactcc cgctcccccc ggtccccctg
     1321 ttcgcgagca cgcagatgaa caacgactca cccgaaaagg tcctcttcct cgaaaagacc
     1381 ggcttttcgc gcgtcattct cccgcgggaa atgaccctca acgagatccg cgcggtgagg
     1441 agtaaaacct ccgtcgatct cgaatgtttc atccacggcg cgctctgcgt gtcttacagc
     1501 gggcagtgtt atctgagtta cgcgatcggc gggaggagcg cgaaccgcgg cgcgtgcgct
     1561 caaccgtgcc ggcgccttta ctcgctcgtc ggcgcggacg gcaaggtttt cgcgggcccc
     1621 aagcacctcc tgtccctgaa agaccttaac cgttccgctt ccctcagcga gctgatcgac
     1681 gcgggtgtct cgtcgttcaa gatcgagggt cgactcaagg acatcgcata cgtgaagaac
     1741 accgtgggct tttaccggcg ggaactggac gccgtcctcg cccggaaaaa gagggcgaag
     1801 agctcttcgg gaacggttat ccccggcttt gtccccgatc ccgccaaaac cttcaaccgt
     1861 ggtttcacgg atttcggcct caaggggccg accgccgggt ggtcgtcccc cggcacgccg
     1921 aaatccctcg gcgagaggat cggaacggtc gcaaaggccg ccaaagacca tttcacgcta
     1981 acgggccccc acgacctcgc gaacggtgac ggaatctgtt tttttgacgc gcgcggggaa
     2041 ctgcgcggct ccgtcgtgaa ctcggtcgcg aacgaccgcg tgtacccgga caagaccggc
     2101 ggcctgacgg ccggcgccgt catttacagg aatcacgacc gcgcgttcct gaaaacgctc
     2161 acggcgaaaa cctcgtgcga gcggcgaatc gccgtgtcgt tcgtactgag cgggaccccg
     2221 gacgggttcc gtctcacggc gcgcgacgag gacgggaaca ccgcggactt ctccgtcccg
     2281 gccgcaaaac agcccgcgga gaaaaaagcg caggccgaag agacgctcgt gaaacagatt
     2341 tcgaagctcc atgacacggc gttcacctgc atctcggtca cgcacacgct tcccgagatc
     2401 tgtttcctgc ccgtgtcgct cctcaacacg ctccgccgcg gcgcgatcga aacactgatc
     2461 gaggcccggg agcgggcccg cccccgggag accgccgcga tccagccgaa cgacgctcct
     2521 tatcccgtga agaccctcga ttaccgggcg aactgcctta accggaaagc cgttgatttc
     2581 ttcaggcgtc acggcgccga agtaacggag atggcggccg aatcgggcct ccccatgcag
     2641 ggccggctcc tgatgcgctc gaaatactgc gtcaaacgcg atctcgggtc gtgcgggaag
     2701 atgacggaac ctctttttct cgcggatgag aacggtcgac gctttcgcct ggcattcgac
     2761 tgcgacgggt gccgcatgct ggtcttcgac gaccgatagg cgaagaccat atcccgcttc
     2821 gcaggtgtga tatggtccga tccgtcc
//
LOCUS       DAOOBX010000101         2844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_39057_length_2844_cov_2.669416, whole genome shotgun sequence.
ACCESSION   DAOOBX010000101 DAOOBX010000000
VERSION     DAOOBX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39057_length_2844_cov_2.669416"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1156
                     /gene="tyrS"
                     /locus_tag="PLY30_02080"
     CDS             2..1156
                     /gene="tyrS"
                     /locus_tag="PLY30_02080"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPW64922.1"
                     /translation="MSEKELEAKLTDSLKKNRPLRIKYGADPSAPDLHLGHTVPLRKL
                     RQLQDLGHLVVFIIGDFTARIGDPSGKSGTRPMLSEEQVKKNALTYQEQVFQILDRSK
                     TEVRYNSEWLDKLTPQAFLKLTAQYTVARILERDDFSKRYKANEPIAIVEFLYPLMQG
                     HDSVVIRSDVELGGTDQKFNLLVGRELQKLDGQEPQVVMTLPLIEGTDGVQKMSKSLG
                     NHIALKDTPREMFGKVMSIPDPLMERFYRYVSALPSEETGAIVLGLKKGELHPREMKA
                     KLAERIVTLFHSIEAGRQASQEFDEIFRKKGLPDEIPTVKIEKREIDIVSLLGEAGLV
                     ASKSEGRRLVEQGGVKVDQEKVSDPKTVLKIEKTIVLQCGKRKFARIEPA"
     gene            1160..2158
                     /locus_tag="PLY30_02085"
     CDS             1160..2158
                     /locus_tag="PLY30_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW64923.1"
                     /translation="MVTVRKPRSAEGVVRFESVTKNYGKRAVVDNLSFEIARGEVVGF
                     LGPNGAGKTTAMRILSGYFAPSSGHVEIEGIDLFKDPRRAKQRVGYLPETVRFYPDMR
                     VREYLRFVTGLKGVPEKRRTDEVEEKITQCGLSEVSHRLIGRLSKGYRQRVGLAQALI
                     GDPSVLILDEPTTGLDPKQITEIRELIRTLGRERAVILSTHILSEVSMVCNRVLMIHQ
                     GQVLASGTVRELEACLKDRDAIFVTVRGPEHERVVTDLLVQIPGAENIRVVERHEGEI
                     RFCLETSHPLDLRPEITELFVKNRLPLLEVRRDQLTLEEIFLKLLKDGWFQMRRAA"
     gene            2264..>2844
                     /locus_tag="PLY30_02090"
     CDS             2264..>2844
                     /locus_tag="PLY30_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPW64924.1"
                     /translation="MRKAFTVAKKECSSLFHSWTGVFTGAFFFLVTGIFFTVLVLSYA
                     KLSMDPRLAGSQTSFPNVSQTHYIFGSFFLNANVILLFLVPILTMRSFSEERKHETLE
                     LLFTYPLSDLEIVAGKLIGLIWFFEILLVPLAGYFLIFHLIGGELDWGPVISGFLGFW
                     ALGTAYLAAGVFVSTLTRNPMISAVGTFALLIV"
BASE COUNT          600 a          797 c          834 g          613 t
ORIGIN      
        1 catctctgaa aaggagcttg aggccaaact cacggattcc ctgaaaaaga accgcccgct
       61 tcgcatcaaa tacggggccg atccttcggc gccggacctt cacctcggcc acaccgtgcc
      121 gcttcgtaaa ctccgccagc ttcaggactt gggacacctc gttgtcttta tcatcggcga
      181 ttttacggcg cgcatagggg acccctccgg aaaatccggg acgcggccca tgctttccga
      241 agaacaggtg aaaaagaacg ccctgaccta tcaggagcag gtcttccaga ttctggaccg
      301 ttcgaaaacc gaagtccgct ataactccga atggctcgac aagcttacac cgcaggcgtt
      361 tttgaaactc accgcgcagt acaccgtggc ccggatcctc gaacgggatg atttttcaaa
      421 acgctacaaa gcgaacgaac ccatcgcgat cgtcgaattt ctgtatccgc tgatgcaggg
      481 gcatgactcc gtcgtgatcc ggtccgatgt cgaactcggc gggacggatc agaaattcaa
      541 cctgctcgtg ggacgggagc ttcagaagct tgacgggcag gaaccgcaag ttgtgatgac
      601 ccttccgttg attgagggaa cggacggtgt ccagaaaatg tcgaaaagtc tcggcaatca
      661 catcgccctc aaggacacgc cccgcgaaat gttcggcaag gtgatgtcga tcccggaccc
      721 gctgatggaa cgtttttatc gctatgtgag cgcgctgccg tccgaagaaa cgggtgcgat
      781 cgtcctgggg ttgaaaaagg gtgaactgca tccccgcgaa atgaaggcga aactcgcgga
      841 acggatcgtg acgctttttc acagtatcga ggcgggccgc caggcttcgc aggaattcga
      901 cgaaatcttc aggaaaaaag gtcttcccga tgagatcccg accgtaaaga tcgaaaaaag
      961 agagatcgat atcgtttcac tccttggcga agccggactg gtcgcgagca agtcggaagg
     1021 gcgccgcctt gtcgagcagg gcggcgtgaa ggtcgaccag gaaaaagttt ctgatccgaa
     1081 aaccgtcctc aaaatcgaaa aaacgatcgt cctgcagtgc gggaagcgca agtttgcccg
     1141 catcgaaccc gcctgagcca tggttactgt tcgcaagccg cgttccgctg aaggggtcgt
     1201 ccgctttgaa agcgtgacga agaattacgg caagcgcgcg gtcgtcgaca atttatcttt
     1261 tgagatcgcg cgcggcgaag tggtggggtt cctcggtccc aacggcgcgg gcaaaacgac
     1321 cgcgatgcgt atcctttccg gttatttcgc tccgtcctcc gggcatgtgg agatcgaggg
     1381 catcgacctg ttcaaggatc cccgccgcgc gaaacagcgg gtcggctatc ttccggaaac
     1441 agtccgcttt tatcccgata tgcgagtgcg ggaatacctg cggtttgtca ccggtctcaa
     1501 gggagtgccc gagaaacgcc gcaccgacga ggtggaggaa aagatcacgc aatgcgggct
     1561 ctccgaggtt tcgcaccgcc tcatcgggcg cctgtcgaaa ggttaccgtc agcgggtcgg
     1621 gctcgcacag gccctgatcg gggatccttc ggttctcatc ctggacgagc cgacgacggg
     1681 gctcgatccc aaacagatca ccgaaatccg ggagctcatc cggacacttg gcagggaacg
     1741 ggccgtgatt ctgtcgaccc acatcctctc cgaggtttcc atggtctgca accgcgtcct
     1801 catgatccat caggggcagg tactcgcgag cggcacggtg cgggagcttg aggcctgtct
     1861 gaaggaccgg gatgccatct tcgtgaccgt tcgcggtccc gaacatgaga gggtcgtgac
     1921 ggaccttctg gtccagattc ccggtgccga aaatatccgg gtggtcgaac gccatgaagg
     1981 cgagatccgg ttctgcctcg agacctcgca cccgctggac cttcgtcccg agatcacgga
     2041 acttttcgtg aaaaaccggc tgcctcttct ggaggttcgc cgggaccagc tgacgctcga
     2101 ggagattttt ctgaagcttc tgaaggacgg atggttccaa atgcggaggg cggcatgagc
     2161 cccgttagag acgggaagcg cagggaccgc gggatgtctg gcggtatttt aatgtctgcc
     2221 gataagtatg ggaggggaga cgctataacg cgtttctaac ggaatgagaa aagcgttcac
     2281 ggtcgccaaa aaggagtgtt cgtccctttt tcattcctgg acgggcgttt tcacgggggc
     2341 gttctttttc ctcgtgaccg gaatcttttt caccgtgctg gtgctgagct acgcgaaact
     2401 gtcgatggac ccgcgtcttg cggggagcca gacgagtttc ccgaacgtca gccagaccca
     2461 ttacatcttc ggttcttttt tcctgaacgc gaacgtgatc cttctttttc tcgttccgat
     2521 cctgacgatg cgctcgttct cggaagagcg caagcatgaa acgctcgaac ttctcttcac
     2581 ttatccgctc tccgatctcg agatcgttgc cggcaagctg atagggctga tctggttctt
     2641 tgagatcctc ctcgtgccgc tcgcgggata tttcctgatc ttccacctga tcggaggcga
     2701 gctggattgg gggccagtca tctcggggtt tctcggtttc tgggccttgg gcaccgctta
     2761 tcttgcggcg ggtgtattcg tgtcgacgct caccaggaac cccatgatca gcgcggtggg
     2821 gacattcgcg cttctcatcg tttt
//
LOCUS       DAOOBX010000102         2821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_39472_length_2821_cov_3.995300, whole genome shotgun sequence.
ACCESSION   DAOOBX010000102 DAOOBX010000000
VERSION     DAOOBX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39472_length_2821_cov_3.995300"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PLY30_02095"
     CDS             complement(<1..1130)
                     /locus_tag="PLY30_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HPW64925.1"
                     /translation="MKTVLVSSKGGAKADILFIGFFKGGKDLQGIKKIEPLFGKVLEA
                     AIKKERFAGKPQEVFASFQPAYRHAAEVVVVGLGEKKDLKPALLRKIAGKIIEVAGTR
                     KAGKIRVLADTFLGDVLEAKTAAGIFAESAMLASYRFLRYKTGKKDALPKFPETFELV
                     FEDKNLAGELKRAVTRAEKIAKAVIYARDLNNEPANVMNPPRLVEESGKLAEEKGLRH
                     TALDLTELTELGMNGILGVNRGSVTPPALIILEHGEEYKENGTVCLVGKGVTFDTGGI
                     SIKPSKGMEEMKFDKSGAIAVIATMGLVADLKLPLRVIGLAPCVENNVANNPQRPGDI
                     IRMYNGKTVEVINTDAEGRLILADALAYCEKYKPDAIIDAAT"
     gene            complement(1149..1502)
                     /locus_tag="PLY30_02100"
     CDS             complement(1149..1502)
                     /locus_tag="PLY30_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPW64926.1"
                     /translation="MDWIAALIGLIGGFTGGLLGVGGGTIYIPLMIMLKGMDTHRAVG
                     TSLMTIIFTGMFGAFFHHRQGMVDLRIAVAMGIFSILGVWLGTRLSVATDELMLRRIF
                     SVFLVVVAFKLFFTK"
     gene            complement(1507..1923)
                     /locus_tag="PLY30_02105"
     CDS             complement(1507..1923)
                     /locus_tag="PLY30_02105"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HPW64927.1"
                     /translation="MMKKGKIYSDGGARGNPGPAAIGVLVCDENDHKLSDHGETIGET
                     TNNVAEYTAVVRGLTLASELGIGEADYFLDSELVARQLSGVYKLKAEHLKPFFVQVKS
                     LEKKFKRVTYFHVPREHAKIRHVDRLVNLALDRAGC"
     gene            complement(1946..2647)
                     /gene="cysE"
                     /locus_tag="PLY30_02110"
     CDS             complement(1946..2647)
                     /gene="cysE"
                     /locus_tag="PLY30_02110"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPW64928.1"
                     /translation="MKNYPFYRVFRDFRACFDRDPAARTPLGVLEVLSLYSGFHAVLF
                     YRFAHILYRLKIPFIPRLISQIARFLTGVEIHPGARIGPGFFIDHGMGVVIGETTEIG
                     ENVTIYQGVTLGGTGKERGKRHPTLGNNVVVGVGAKILGNIRIGNHVKVGGGSVVIHS
                     VPDYCTVVGVPGEIVRIDGKKPGEIDLEHGDLPDPVKELKNRLRALQKEVEQIEAQHK
                     GSKNARRSVDGGEKQ"
BASE COUNT          555 a          859 c          790 g          617 t
ORIGIN      
        1 aacgtggccg cgtcgatgat cgcgtccggc ttgtattttt cgcagtacgc gagcgcgtcc
       61 gcgagaatca gccgtccttc cgcgtccgtg ttgatgacct caacggtctt cccgttgtac
      121 atcctgatga tatcgcctgg acgctgggga ttgttcgcaa cgttgttctc cacgcacggc
      181 gcaaggccga taacacggag cgggagcttg aggtccgcga caagccccat cgtcgcgatc
      241 acggcgatcg cgccggactt gtcgaacttc atctcctcca tccccttcga gggcttgatc
      301 gagatgccgc ctgtgtcaaa ggtgacgccc ttgccgacga ggcacacggt cccgttctct
      361 ttatattcct ctccgtgctc caggataatg agcgcggggg gcgtcacgct ccccctgttc
      421 acaccgagaa tgccgttcat tccgagttcg gtcagctccg tgagatcgag cgcggtgtgc
      481 cggagcccct tttcctccgc aagcttcccg gattcctcga cgagccgcgg cgggttcata
      541 acgttggccg gctcgttgtt gaggtcgcgg gcatagatca cggctttcgc gatcttttcc
      601 gcgcgggtca cggcgcgctt cagttcgccg gcaaggttct tgtcttcgaa gaccagctcg
      661 aacgtttccg ggaatttcgg aagcgcgtcc tttttcccgg tcttgtaccg aaggaaacga
      721 taagaggcaa gcatcgcgct ttccgcgaag atcccggcag ccgttttcgc ctccagaaca
      781 tcccccagaa aggtgtccgc gagcacgcgg attttcccgg cctttctcgt gccggcgacc
      841 tcaatgattt ttccggcaat cttgcggagc aatgccggct tcaggtcctt cttttccccg
      901 agtccaacca cgaccacctc cgcggcgtgc cgataggccg gctggaagga agcgaacacc
      961 tcctgcggct ttcccgcgaa ccgttctttc ttgatcgcgg cctcgagcac ctttccgaaa
     1021 agcggctcga tctttttgat tccctgcagg tctttcccgc ccttgaagaa cccgatgaaa
     1081 aggatgtcgg ctttcgcgcc gcctttggaa gaaacgagta ccgttttcat gtcacaacct
     1141 ccctgggatt atttcgtgaa gaataatttg aacgcgacga caaccagaaa aacggaaaaa
     1201 atcctccgga gcattagctc gtcggtcgcg accgagagcc tggttcccag ccagacgccg
     1261 aggatgctga agatgcccat cgccacggcg atcctgaggt cgaccatgcc ttgccggtgg
     1321 tggaagaatg cgccgaacat gcccgtgaaa atgatcgtca tgagcgaggt cccgacggcc
     1381 cggtgcgtgt ccatcccctt caacatgatc atgagcggaa tgtagatcgt cccgccgcca
     1441 acgccaagga gcccgcccgt gaaaccgccg atcaacccga tcaacgccgc aatccagtcc
     1501 atggcctcag caccccgccc ggtccagcgc aagattgaca aggcgatcga catgcctgat
     1561 ctttgcgtgt tcgcgcggca catggaagta agtcacgcgc ttgaattttt tttcgaggga
     1621 cttgacctga acgaagaacg gcttgagatg ttcggccttg agcttataga ccccagagag
     1681 ctggcgcgcc acaagctcgc tgtcgaggaa ataatccgct tctccgatgc cgagctcgga
     1741 ggcgagcgtc aggccgcgaa ccacggccgt atattccgcc acattgttcg tcgtttcacc
     1801 gattgtttcg ccgtgatcgg aaagtttatg gtcgttctcg tcacagacca gcacgccgat
     1861 cgcggccggt ccggggttgc cgcgggcgcc cccgtcggaa tagatttttc cctttttcat
     1921 catggcatga cctgcagggg atcgttcatt gtttttcgcc gccgtccacg gagcgccggg
     1981 cgttcttcga tcccttgtgc tgcgcctcga tctgctcgac ttccttctgg agcgcgcgaa
     2041 gacggttctt gagttccttc acggggtccg gaagatcgcc gtgctcgaga tcgatctccc
     2101 cgggtttttt cccgtcgata cggactatct cgcccggcac gccaacgacc gtgcagtaat
     2161 ccggcacgga atggatcacg accgatcctc cgccaacctt gacgtggttc ccgatccgga
     2221 tattgccgag aatcttcgcg ccgacgccga caaccacatt gttaccgagc gtcggatggc
     2281 gtttgccgcg ttctttcccg gtgccgccga gcgtcacgcc ctggtagatc gtgacgtttt
     2341 cgccgatctc ggtcgtctct ccgatcacga cgcccatgcc gtgatcgatg aaaaatcccg
     2401 ggccgatcct ggcaccgggg tggatctcga caccggtcaa aaaacgcgcg atctgcgaga
     2461 taaggcgcgg gataaaaggg atcttcaggc gatacaggat atgcgcgaag cggtagaaaa
     2521 gcaccgcgtg gaaaccggaa taaagggaga ggacctccag gaccccaagg ggtgtacgcg
     2581 ccgccggatc gcgatcaaag caggccctaa aatcacggaa aacccggtaa aacggatagt
     2641 ttttcatggc gggcggtatt atttcagatt atagggtgac atgcaaggga ctcgggacct
     2701 gtaacggttt cttcacccgg tcctgagaga taagccccgg tttttaaaag tcaaatcacg
     2761 tcccctgcgg ttttgtccgc ggggaccggt taaaaatcaa atcttgcggt caggtaatag
     2821 g
//
LOCUS       DAOOBX010000103         2815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_39591_length_2815_cov_3.002899, whole genome shotgun sequence.
ACCESSION   DAOOBX010000103 DAOOBX010000000
VERSION     DAOOBX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39591_length_2815_cov_3.002899"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2661)
                     /locus_tag="PLY30_02115"
     CDS             complement(<1..2661)
                     /locus_tag="PLY30_02115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW64929.1"
                     /translation="MIRDFRALSDAVSTELRLAAPVLSGMDADPAFRSRIDSLYEAAY
                     AFYAHAHEREAVFYEKIDAVMSARGERAAVLITGGFHTGGITDLFRDHDVSYGVLTPR
                     LMEKSDEKAYRDNMLERGATVFDIANLEAFNIMQDDDVIRSQIGPEGLKAFRKYLTST
                     LVKILARHPQHLARAIKAISERSFREHGIRWVERRNGKDSVVVLEKVGKNEEDVEEIT
                     NITDKDGKVYALRFSPLENGKYAMRAALMDPVSVTPALAAQAPTAQTPVGSRAGARPE
                     SDQKQDVGTRPESRRDVEVNQASVQSGPMTLQVMNVRVGPWVLPAFASKFIRSVFDRL
                     AMWGVGPNISEGAGYYRGVAGNYGIDLDDEIAKRVAALDGARPLRVLDLGAGTAGYLQ
                     RLKDRYGDRVEAIAVDSSYPEQKRTRRGVKMIGKDAPDAVREFRERGEEFDVVLSINA
                     NSAAREFLLGSGPVLLNSNSLVFVHGADFLNEDHISSLEKQGYSVANGGTVTRKTPPS
                     AAAKQPGSRSEMRVPLPVPDELVLDEKPDFDAWMNRLGARGIRPAEPVIAITAKLFSA
                     RKVLPYRDFEQRLRDLEPKLIDALRNAGSYAVLLHKPHTSRRWVLSHLRVGAPDGVRA
                     PGGLFYEGESEDHLKKASRFMTFVVVDDVAYSGRQIRETIEKVGRVNPGAKFIIVVPY
                     MTNAAAKLIKQRPLLHPEPGIRMLPKNVTVNVLPFQKLPTISDVLPRGKKGEMGEIDV
                     FELNKGLFGADLRVDGKPYDVANLSVVIPEWKIPDQFSLLQLLIPQRLVDQPFLPPYK
                     DPRTEYYREEEKWFQGLKPRAERKEVRRTPESEIVGQAHEVTARQEARAERRTQPARG
                     IREGLRSGEIAAETSSRFEQTISAAHRVWYE"
BASE COUNT          578 a          875 c          751 g          611 t
ORIGIN      
        1 ttcgtaccag acccgatgag cagcggaaat cgtttgttcg aagcgagagg atgtctcagc
       61 ggcgatctcc ccgctccgca aaccttctct tatgccgcgg gccggctgcg tccgccgctc
      121 ggcacgtgct tcctgccggg ccgtcacttc atgcgcttgt ccgacaatct cactctccgg
      181 tgtccggcga acttctttac gttccgcgcg gggttttagg ccttggaacc acttttcttc
      241 ctcccggtaa tactccgtgc gcggatcttt ataaggcgga agaaatggct ggtcaaccag
      301 gcgctgggga atcaggagct gaaggaggga gaattggtcg gggatcttcc actccgggat
      361 cacaacggaa agattggcga catcgtaggg cttgccgtca acccgaagat cggcgccaaa
      421 gagccccttg ttcagctcga acacatcaat ttcacccatt tcaccctttt taccccttgg
      481 caggacatcc gaaatggttg gcaacttctg aaaaggaagc acgttcaccg tcacgttttt
      541 cggcaacata cgaatcccgg gttcaggatg aagcagaggc cgttgcttta tcaatttggc
      601 cgccgcgttc gtcatgtaag gcactacaat aatgaacttc gcgcccgggt tgaccctccc
      661 gaccttttcg atcgtttccc ggatttgccg gccactgtac gccacgtcat ccacaacaac
      721 aaacgtcatg aaacgcgagg ctttcttcaa atgatcctcc gactcaccct cgtaaaacag
      781 accccccggc gcccggacac cgtcaggggc accgacacgg agatggctca gaacccagcg
      841 ccgggacgta tggggtttgt gcaacagcac ggcgtatgag ccggcgttcc gaagcgcgtc
      901 gatcagtttc ggttcaaggt cccgcaacct ctgttcaaag tcccgatatg gaagcacctt
      961 tcgagccgaa aaaagcttgg ctgtaatcgc gatgaccggc tccgcggggc gaatgcctct
     1021 ggcccccaat cggttcatcc aggcgtcgaa atcgggcttc tcgtcgagca ctaattcatc
     1081 cggaaccggc agcggcaccc tcatttcact gcgactgccc ggctgttttg ccgcggccga
     1141 gggtggagtt ttccgggtga ctgttcctcc gttagcaacc gagtatccct gtttttcaag
     1201 agaggagata tgatcctcat ttaaaaaatc agccccgtga acaaaaacaa gggagttgga
     1261 gttgagaagg acagggcccg acccaagaag aaattcccgc gccgcagaat tggcattaat
     1321 ggataagacc acatcgaatt cttctccccg ctcacggaat tcccggacgg catccggggc
     1381 gtctttgccg atcattttca cgcccctgcg cgttcgcttt tgttcaggat atgaagaatc
     1441 caccgcgatc gcttcgaccc gatccccata gcgatccttc aaccgctgta aatagcccgc
     1501 agtaccggct cccaagtcca gaacacgtaa cggccgtgcc ccgtcgaggg ccgcgactct
     1561 ttttgcgatc tcatcatcaa gatcgatccc atagttcccg gccacccccc ggtaataccc
     1621 tgcgccctcg ctgatgtttg gccccactcc ccacatagcc aggcgatcaa aaacggaccg
     1681 gatgaacttg ctcgcgaaag cgggcaacac ccagggtccg acacgaacgt tcattacctg
     1741 tagtgtcatc ggaccacttt ggacagaggc ttgatttacc tcaacatccc tccgcgattc
     1801 cggacgggtg ccgacgtcct gtttttgatc cgattcaggt cgggccccag cgcgcgaacc
     1861 gacgggagtt tgagcggtcg gtgcctgcgc ggcgagtgcc ggagtcacgg acacgggatc
     1921 catgagcgcc gcccgcatcg catacttgcc gttctcgagc ggtgaaaacc tcagggcgta
     1981 cacctttccg tctttatccg taatattcgt gatctcctca acatcctctt cgttctttcc
     2041 gaccttttcg aggacaacca cggaatcctt gccgttccgc ctctcgaccc aacggattcc
     2101 gtgttccctg aaggagcgtt cactgatcgc cttaatagcc ctcgccaggt gctgcggatg
     2161 acgggccaga attttgacga gcgtgctcgt gaggtatttc cggaacgcct tcaatccctc
     2221 ggggccgatc tgagaccgga tcacgtcatc atcctgcatg atgttgaagg cttcgaggtt
     2281 cgcgatatca aacaccgtgg cgcctcgctc aagcatgttg tcgcggtagg ccttctcatc
     2341 cgacttctcc atgagccggg gcgtaagcac gccgtagctc acgtcatggt cgcggaagag
     2401 gtccgtgata ccgcccgtgt ggaagccgcc ggtgatgagt accgcggccc gctctccgcg
     2461 cgcgctcatg acggcatcga tcttttcgta gaacaccgct tcgcgctcat gcgcgtgggc
     2521 gtagaacgcg taggcggcct catagaggga atcaatcctc gagcggaacg cagggtccgc
     2581 gtccatcccg gaaagaacgg gagccgcgag ccgcagttcc gtactaaccg catccgagag
     2641 tgccctgaag tcgcggatca tcgcgtctat cgcgaccgcg tcctttttcg cctagatctc
     2701 ctgccagtca cggcgcgcaa gttcgaggtt gagaagttta cggatcaatt ctgtccggcg
     2761 attgagttcc acaagcttcc gctgagcggg cgacgcggcc agcttctcaa gaatc
//
LOCUS       DAOOBX010000104         2814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_39605_length_2814_cov_2.694092, whole genome shotgun sequence.
ACCESSION   DAOOBX010000104 DAOOBX010000000
VERSION     DAOOBX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39605_length_2814_cov_2.694092"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLY30_02120"
     CDS             <1..772
                     /locus_tag="PLY30_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPW64930.1"
                     /translation="AEKIFRELDASMKAPLSVTDIKSAELIKHASNSFLATKISFANG
                     LSRICDAVGADVEKVTLGMGLDPRIGSQFLHAGIGFGGACFPKDLKAFLHISNELGVS
                     FDLLAEVIRINETQAQYFVQKIIRNMRSLKGKTIGVLGLAFKGDTDDMRNAPSLAIIP
                     ELQKAGAQIKAYDPQAMPGSRTLFRNLCFAPDLYSAVKDAHAIVLLTEWKKFQNMDCR
                     RVRKLMKGKFFFDGRNMFPPPEMKRLGFHYVSIGRPSA"
     gene            810..1541
                     /locus_tag="PLY30_02125"
     CDS             810..1541
                     /locus_tag="PLY30_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPW64931.1"
                     /translation="MKGVILAGGLGSRLYPLTKVTNKHLLPVYDRPMVYYPIETLVKA
                     GVTDILLVTGGNNAGDFLRLLGNGKQFGLKHINYTYQEGEGGIAQALSLAEHFADGDK
                     IVVILGNNIIEADISPQVRRFRTQETGARLLLKKVSDPERFGVATVRGNRITSIIEKP
                     RRPKSDLAVVGIYMYDADVFNHIRTLKPSRRGELEITDVNNWYLKRGDLEYDVLPGYW
                     TDAGTFESLYRANSLVARKKGRSGK"
     gene            1538..2515
                     /gene="rfbB"
                     /locus_tag="PLY30_02130"
     CDS             1538..2515
                     /gene="rfbB"
                     /locus_tag="PLY30_02130"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPW64932.1"
                     /translation="MKRLLVTGGCGFIGSNFIRLVMKERPGYSVVNLDKLSYSGNPEN
                     LRDLAQNTRYTFVKGDICDKKLVAKVMSRVDAVVHFAAETHVDRSIAGADEFLKTNIL
                     GTASLLEASRACGIKRFVHMSTDEVYGSLAKGKADEEHRLEPNSPYSASKAGGDLLVR
                     SFRVTHKHPAMIIRCTNNYGPYQFPEKVIPLFVTNLLEGKTVPLYGQGRNRRDWIFVE
                     DACRAVLLVLEKGNPGEIYNVGSDHERSNLQLTRAILKELGRGEDMIRRVADRPGHDF
                     RYALNFNKLRKLGFKPRWSFEEGLRTTVGWYVANEWWWLPLKKDKFTVK"
     gene            2517..>2814
                     /locus_tag="PLY30_02135"
     CDS             2517..>2814
                     /locus_tag="PLY30_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HPW64933.1"
                     /translation="MPTEAFGILITGAGGMLGTDLVRALENDFEIIGLDKKPAPHLER
                     PLEICDLLQERKLREVFMSRNPGLVIHCAALTDVDYCEDHAEEAVAQNVEATRNI"
BASE COUNT          640 a          819 c          803 g          552 t
ORIGIN      
        1 cgccgaaaag atcttccgcg agcttgacgc cagcatgaaa gcccccctca gcgtgaccga
       61 catcaaaagc gccgagctga tcaagcacgc ctccaactcg ttcctcgcga cgaagatatc
      121 gttcgcgaac ggcctctcgc gcatttgtga cgccgtcggg gccgatgtcg agaaggtcac
      181 actcggtatg gggctggacc cgaggatcgg atcgcagttc ctgcatgccg gtatcggctt
      241 cggaggcgcg tgcttcccga aggatctgaa ggcgttcctg cacatctcga acgagctcgg
      301 ggtcagcttt gaccttctcg cggaggtgat ccggatcaac gagacccagg cgcagtattt
      361 cgtgcaaaag atcatccgaa acatgcgttc tctcaagggg aaaacaatcg gagttctcgg
      421 cctcgcgttc aaaggtgata cggacgatat gcggaatgcc ccctcgctcg caattatccc
      481 cgagttgcaa aaagcgggcg cgcagatcaa ggcgtacgat ccacaggcca tgcccgggtc
      541 ccggaccctt ttcaggaacc tttgtttcgc gccggacctg tactcggccg tcaaagacgc
      601 tcatgccatc gtactgctca cggaatggaa gaagttccag aacatggact gccgccgggt
      661 ccgcaagctt atgaagggaa aattcttttt cgacggacgg aatatgtttc cgccgcccga
      721 aatgaaacgg ctgggctttc attatgtcag catcggcagg ccgtccgctt gagccacgga
      781 cgcttccgga gaaaacagag gcgaatatca tgaaaggcgt cattcttgcg ggagggctag
      841 gttcaagact ttatccgctc acgaaggtca ccaacaagca tcttctgccc gtctacgacc
      901 gtcccatggt ctattacccg atcgagacgc tggtcaaggc gggggtcacc gatatcctgc
      961 tcgtgacggg cgggaacaat gcgggggatt tcctgaggct tctcgggaac gggaagcagt
     1021 tcggactgaa acacatcaac tacacgtacc aggaaggcga gggcggtatc gcgcaggcac
     1081 tcagtcttgc ggaacatttc gccgacggcg acaagatcgt cgtgatcctg ggtaacaaca
     1141 tcatcgaggc cgacatctcg ccccaggtcc ggcggttccg gacacaggag accggcgcgc
     1201 ggcttttact gaaaaaagtg agcgacccgg aacgcttcgg tgtcgcgacc gtccggggca
     1261 acaggatcac gtccatcatc gaaaaaccca gacgcccgaa gagcgatctc gctgtggtcg
     1321 gcatctatat gtatgacgcg gacgttttca atcacatccg gacactgaaa ccttccaggc
     1381 gcggggagct tgagattacg gacgtcaata actggtacct gaagcgcggg gatcttgaat
     1441 acgatgttct tccgggatac tggacggacg ccgggacctt tgagtctctt taccgggcca
     1501 attccctcgt tgccaggaaa aaagggaggt cgggcaaatg aaaaggcttc tcgttacggg
     1561 cgggtgcggc ttcatcggtt ccaatttcat ccgccttgtc atgaaagaac gtccggggta
     1621 ttcggttgtc aacctggaca agcttagcta ttccgggaat cccgagaacc tgagagatct
     1681 cgcccaaaac acccgctata ccttcgtgaa gggcgatatc tgcgacaaaa aactcgtcgc
     1741 gaaagtcatg tcgcgggtcg atgcggtcgt ccatttcgcg gccgaaacgc atgttgaccg
     1801 ttctatcgcc ggtgccgacg aattcctgaa gacgaacatc ctcggcacgg cgtcgctcct
     1861 tgaagcgtcc cgcgcctgtg ggatcaaacg gtttgtccat atgtcgacag atgaggtcta
     1921 cggttccctc gcgaaaggga aagcggacga ggagcaccgt ctcgagccga acagtcccta
     1981 ttccgcgtcc aaggcgggcg gcgacctcct cgtgcgctcc ttccgggtca cgcacaaaca
     2041 tcccgcgatg attatccgct gcacgaacaa ctacggaccg tatcaatttc cggagaaggt
     2101 aatcccgctt ttcgtgacca atctccttga aggcaagacc gtccctctct acgggcaggg
     2161 caggaaccgg cgcgactgga tcttcgtcga ggatgcctgc cgcgcggtcc tcctggtcct
     2221 ggaaaaagga aatccgggcg agatttataa tgttggctct gaccatgaac ggtccaatct
     2281 gcagctgacc cgcgcgatcc tgaaggaact cggccgcggt gaggacatga tccgcagggt
     2341 ggccgaccgc cccggccacg atttccgtta cgcgctcaat ttcaataagc tgaggaagct
     2401 cggtttcaaa ccgcgctgga gcttcgaaga agggctccgg accaccgtcg gctggtatgt
     2461 cgccaatgag tggtggtggc tccccctcaa aaaagacaaa ttcacggtca agtaatatgc
     2521 ccacggaagc cttcggaatc ctgatcaccg gcgcgggagg gatgctcgga acggacctcg
     2581 tccgcgccct ggaaaacgac ttcgagatca tcggcctcga taaaaaacct gcgccgcatc
     2641 tcgaacgccc tctcgagatt tgcgacctcc tccaggaacg gaaactccgg gaagttttca
     2701 tgtcccggaa tcccggcctt gtcatccatt gcgcggcgct cacggatgtc gattactgcg
     2761 aagatcacgc ggaagaagcg gtcgcgcaaa acgtggaagc cacacggaac atcg
//
LOCUS       DAOOBX010000105         2801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_39821_length_2801_cov_2.873270, whole genome shotgun sequence.
ACCESSION   DAOOBX010000105 DAOOBX010000000
VERSION     DAOOBX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39821_length_2801_cov_2.873270"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLY30_02140"
     CDS             complement(<1..762)
                     /locus_tag="PLY30_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW64934.1"
                     /translation="MYESFFGLQEPPFNVTPDPRFVFLSRHHQAALSAMKYGVERRSG
                     FIQITGEIGAGKTTISRMFLQQIGENVHTSLVFNPKLSELELLEVIVEDFGLKPLSRK
                     RKGYFDALNRFLLEESSKGFNAVLIIDEAQLLPPKALEQIRLLSNLETTSQKLLQIIL
                     MGQPELNDLLNRPDLKQLKQRIAVRCHLPELNLEELGQYLTHRLSVAGVSREIFMPDA
                     VRRVHELSSGIPRVVNILADRALLAAFTAESPCVDA"
     gene            complement(831..>2801)
                     /locus_tag="PLY30_02145"
     CDS             complement(831..>2801)
                     /locus_tag="PLY30_02145"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPW64935.1"
                     /translation="HYWRLNPNYWKDVLCRVKEMGLTIVSTYVPWDFHEYKRGSFDFR
                     GETDEARNLKAFLDLTRREGLWLVIRPGPYIYSEWPHDGVPDYAYKYHRLHPRFLEFA
                     SVYIREVTKVFKPYLATRRGGHIILLQADNEIDPWPDVFGQQYGLSGEAGLFQDFIRK
                     LYDNDLGALNRAWGTAYGSFREASPFIATMLAGEKGIPLKGDKELKRNLDYFKFKYDY
                     TLKVAKWSVEAYRKLGIDVPIYLNTYPFFYAHDWADFQSVADMVGVDLYPANELSEDE
                     FEHRKFCDKVRYLASVSRLPFIAEFQSGVWHGRHYETSVLTPNHYRMICLSAFLAGVK
                     GWNWYMLVNRDNWYMSPINEWGRVRGELYDVFKQIVRAFKALKPASLRKLTEVGVTFN
                     PLQYAARTLTHNSPILQALYKGDVDYEVCDPRRSVPQKKVIFYSGNQWLDMKAHENLR
                     AYVEKGGTLVAFRSYPRKDQDFNPYQTIGFEDPERILFEFKRQFTISLDKGRPAVPVN
                     SSVYVFKAERCRKIQADMGGYGRQTIGYVKSVGKGRILHLGVEPTPELVLEILRYLET
                     PLFAYSITRDVKTAIFRRGAKHYLVVVNNGNEDKSATVFLPALDGDRRGMIARDLMNG
                     EVFDQSKERRRIFSFPVSRKDGRLFELSRCSA"
BASE COUNT          618 a          804 c          812 g          567 t
ORIGIN      
        1 cgcatcgaca cacggacttt ccgccgtaaa cgcggccagg agcgcgcgat ccgccaggat
       61 attgacaaca cgcggaatcc ccgatgaaag ttcatgcaca cgccggacag cgtccggcat
      121 gaagatctcg cgggacacac ccgcgacgga caaccggtga gtgagatact gccccagctc
      181 ttcgaggttc agttcgggca ggtgacagcg aacggcgata cgctgtttga gctgtttgag
      241 gtcgggacgg ttcaaaaggt cgttcagctc gggctgcccc atcaggatga tctgaagaag
      301 tttctgggac gttgtctcca gattggaaag gaggcggatt tgctcgagcg ctttcggggg
      361 caggagttga gcttcatcaa tgatgagcac ggcattgaac cctttcgaac tctcctcgag
      421 aaggaaccgg ttcagcgcgt cgaaataacc cttgcgtttg cgggaaagcg gcttgagacc
      481 gaaatcctcc acgatgacct cgagaagttc gagctcggaa agtttgggat tgaaaacaag
      541 cgacgtgtgg acgttctcgc cgatctgctg gaggaacatc cgcgagatcg tggtcttccc
      601 cgccccgatt tccccggtga tctggatgaa accggaacgg cgctccacgc cgtacttcat
      661 ggccgaaagg gcggcctgat gatgacggct caaaaaaacg aacctcgggt ccggcgtaac
      721 attgaatggc ggttcctgca ggccaaaaaa agattcgtac atgcgctctc ctgtccgtta
      781 aaagaacgac acgggaaggc ccaagcttga gggaaaagcc gcgaggctgg tcaggcagaa
      841 caccgggaga gctcgaagag gcggccgtct ttgcgggaaa cgggaaaact gaaaatccgg
      901 cggcgctctt tcgactggtc gaagacctcg ccgttcatca gatcgcgagc gatcatcccc
      961 ctccggtcgc cgtcaagggc gggaaggaac acggtcgcgc tcttgtcctc attcccgtta
     1021 ttgacgacca ccaggtagtg cttcgcgccg cggcggaaga tggccgtctt gacatcgcgg
     1081 gtgatcgaat aggcaaaaag cggcgtctcc agataccgca ggatttccag gaccagttcc
     1141 ggcgtcggct ccacgcccag gtggaggatc cggcccttgc ccacagattt gacatagccg
     1201 atcgtctgac ggccgtagcc gcccatgtcc gcctggatct tccgacacct ttccgccttg
     1261 aagacataga cggagctatt caccggcacg gccggccggc ccttgtcgag gctgatcgtg
     1321 aactgccgtt tgaactcgaa gagaatccgt tccgggtcct cgaaaccaat cgtctggtac
     1381 gggttgaaat cctggtcctt ccgcggatag ctccggaacg cgaccagcgt cccgcccttt
     1441 tcgacatacg cgcggaggtt ctcgtgggcc ttcatgtcga gccactggtt tcccgaatag
     1501 aatatgacct tcttctgcgg caccgaacgg cgcgggtcac aaacctcata atccacgtcc
     1561 cccttataaa gggcctggag aatcgggctg ttatgggtca gcgtcctcgc cgcgtactgg
     1621 agcgggttga aggtcacgcc gacctcggtg agtttcctga gcgacgcggg tttcagcgcc
     1681 ttgaaggcgc ggacgatctg cttgaaaaca tcgtagagtt cgccgcgcac ccggccccat
     1741 tcgttgatcg gcgacatgta ccagttgtcg cggttcacga gcatgtacca gttccagcct
     1801 ttgacgccgg cgaggaatgc ggaaaggcag atcatccggt aatggttcgg ggtcagcaca
     1861 ctcgtctcat aatggcgtcc gtgccaaacg ccggactgaa actcggcgat gaacggcaac
     1921 cgcgaaacac tcgcaaggta ccgaaccttg tcgcagaact tcctgtgttc gaattcgtcc
     1981 tcggaaagct cgttggcggg atacagatca acgccgacca tatcggcaac ggactggaaa
     2041 tcggcccaat cgtgggcgta gaagaaagga tacgtgttca ggtagatggg aacatcgatg
     2101 ccgagtttcc ggtaggcctc gacgctccac ttcgccacct tcagcgtgta atcgtattta
     2161 aacttgaaat aatcgagatt ccgcttgagc tccttgtcgc ccttgagcgg gattcccttt
     2221 tctccggcaa gcatcgtggc aataaaaggc gacgcctccc ggaacgaacc gtacgcggta
     2281 ccccacgctc gattcagcgc cccgaggtcg ttatcgtaaa gttttctgat gaaatcctgg
     2341 aaaagaccgg cctccccgct caatccgtac tgctgtccga aaacatcggg ccacggatcg
     2401 atctcgttgt ccgcctggag caggataata tgtcccccgc ggcgggtcgc gagatatggc
     2461 ttgaagacct tcgtgacttc cctgatatag accgaggcga actcgaggaa cctgggatgg
     2521 agacgatgat atttgtacgc ataatcggga acaccgtcgt gcggccattc gctgtagatg
     2581 tagggccccg ggcgtatgac cagccacaag ccttccctgc gggtcaggtc gaggaacgcc
     2641 ttgaggttcc tcgcctcgtc ggtttcgccc cggaaatcaa acgacccccg cttatactca
     2701 tggaaatccc acggtacgta cgtggagaca atggtgagcc ccatctcttt cacgcgacaa
     2761 agaacgtctt tccagtaatt ggggttgagg cgccagtaat g
//
LOCUS       DAOOBX010000106         2777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_40249_length_2777_cov_3.389420, whole genome shotgun sequence.
ACCESSION   DAOOBX010000106 DAOOBX010000000
VERSION     DAOOBX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40249_length_2777_cov_3.389420"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="PLY30_02150"
     CDS             complement(<1..1482)
                     /locus_tag="PLY30_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPW64936.1"
                     /translation="MSKEKNYKDTLNLPQTLFPMKANLVQREPEMLRGWESDGIYRKV
                     REKSAGKPKYILHDGPPYANGKIHIGHALNKVLKDLIVKFRTMRGHDALYVPGWDCHG
                     LPIEHACLKEMGKRKEDVDRVEFRKQARKYAERFIGTQREEFKRLGIFAEWEKPYLTM
                     NFEYQAAIADSFLRLYEDGYIEQRLKPVPWCFDCETALADAELEYEDKVSKSVYVKFR
                     LVKDSLNGKVNPVELRKFGERPVYALVWTTTPWTLPANVGIALHPELSYSFLDMGDEV
                     WFLADARVPFLRSLALKAPLMESIVNVKGVVLDKAEYEHPFLSRKGRAILADYVSSED
                     GTGIVHIAPGHGEEDYKFGHLDNGLAILSPVDAKGRFTEEFDLCRGEHVFKANPKIIE
                     ILKEKKLLLHEADHPHSYPHCWRCKKPILFRATTQWFMKIDHKDLRQKMLGAIQKDIR
                     FIPGWGKNRIAAMVETRPEWCLSRQRYWGVPIPVVRCARCTGVH"
     gene            complement(1513..2355)
                     /locus_tag="PLY30_02155"
     CDS             complement(1513..2355)
                     /locus_tag="PLY30_02155"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPW64937.1"
                     /translation="MKRFCQPKGGLMKHIGDCVKFIRRQTDFRPEIALILGTGLGQLA
                     DRIRTVRVFPYEKLPHFPRSTVQSHKGQLVLGEIGGKRVAAMEGRFHFYEGYSLEEVT
                     FAVRVLRELGAKALVVSNAAGGLNLEFGKGEIVLITDHINFMGVNPLIGPNEDKLGPR
                     FPDMNEPYSKRLIDLASMAAAEVKVPVRRGVYIGVTGPCLETRAEYRMMRNFGADLVG
                     MSTVPEVIVAVHMGMEVLGVSVVTDRCDPDHLEPCDIKEIIKTANEAGPKLDKLITKT
                     IEKF"
     gene            complement(2363..2668)
                     /locus_tag="PLY30_02160"
     CDS             complement(2363..2668)
                     /locus_tag="PLY30_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPW64938.1"
                     /translation="MFIFAEIFKALAMLVNGITTILYWMLFARIIISWFPVDPYSSVV
                     QFLMQVTDPILEPLRKIPIRIGPMDFSPILAFILLVFVNRVLVTLLLSVAYKIQGGM"
BASE COUNT          592 a          845 c          730 g          610 t
ORIGIN      
        1 atgaacaccg gtacacctgg cgcaccgcac aacggggatc ggaacacccc agtagcgctg
       61 ccgcgaaagg caccactccg gacgcgtctc gaccatcgcg gcaatgcgat tcttgcccca
      121 cccgggaata aagcggatat ccttctgaat cgccccgagc attttctgac ggaggtcctt
      181 gtgatcgatc ttcatgaacc actgcgttgt cgcacggaaa agaatcggct tcttgcaacg
      241 ccagcaatgc gggtaggaat gcgggtgatc ggcctcatga agcaaaagtt ttttctcttt
      301 cagaatctcg atgatcttcg ggttggcctt gaaaacatgc tccccgcggc aaagatcgaa
      361 ctcctccgtg aacctcccct tcgcgtccac cggtgaaagg atcgcgaggc cgttatcaag
      421 gtggccaaac ttgtagtcct cttcaccgtg gcccggggca atatggacga tgccggtgcc
      481 atcctcgctc gaaacgtaat cggcaagaat cgcgcgccct tttcgggaaa ggaacgggtg
      541 ctcgtattcc gccttgtcca gcacgactcc cttaacattg acgatggact ccatgagcgg
      601 ggccttcagg gcaagcgaac ggaggaacgg gaccctcgcg tccgcgagga accagacctc
      661 gtcacccata tcgaggaaag aataggagag ctcgggatgg agcgcgatcc cgacgttcgc
      721 gggaagcgtc cagggcgttg tcgtccagac gagcgcgtag accggccgct cgccgaattt
      781 gcggagttcg accggattca ccttcccgtt cagcgagtcc tttacgagcc ggaatttcac
      841 atagacggat ttcgagacct tgtcctcgta ttcaagctcg gcgtcggcga gggcggtttc
      901 gcagtcaaag caccacggca ccggtttcag ccgctgttcg atgtagccgt cctcgtaaag
      961 gcggaggaag gaatccgcga tcgcggcctg atactcgaaa ttcatcgtaa gataaggctt
     1021 ttcccattcc gcgaagattc cgagccgttt gaactcttcg cgctgggtcc cgatgaaccg
     1081 ttcggcgtat tttcgggcct gcttccggaa ctcgacccgg tccacatcct ccttgcgctt
     1141 gcccatctct ttcaggcagg catgctcgat cgggagacca tggcaatccc accccggcac
     1201 ataaagggca tcgtggccgc gcattgtccg gaacttcacg atcaggtctt tcaggacctt
     1261 gttcagggca tgtccgatgt ggattttccc gttcgcgtac ggagggccgt cgtgaaggat
     1321 atatttcggt tttcccgcgg atttttcgcg aaccttcctg tagatgccat cactctccca
     1381 ccccctcaac atctcaggct ccctctgaac gaggttcgcc ttcatcggga acagcgtctg
     1441 cggtagattt aacgtgtcct tgtagttctt ctctttactc atggcaactc cgtacgccgc
     1501 acttcgtacc tgttaaaact tttcgattgt tttcgtaatc agtttatcca gcttcgggcc
     1561 ggcttcgttt gcggtcttga tgatctcctt gatgtcgcaa ggctcaaggt ggtcgggatc
     1621 gcacctatcc gtcaccacgc tcacgccgag cacttccatg cccatatgaa cggccacgat
     1681 cacctccggc accgtcgaca tcccgacaag gtccgcgcca aagtttcgca tcatacggta
     1741 ctccgcgcgc gtttcaaggc acggccccgt gaccccgata tagacgcccc ggcgaaccgg
     1801 caccttcact tcagccgccg ccatcgatgc cagatcgatc agccgtttgg agtaaggctc
     1861 attcatgtcc gggaaacgcg gcccgagttt gtcttcgttc ggcccgataa gcggattcac
     1921 gcccatgaag ttgatatggt ccgtgatcag aacaatctcg cctttgccga attcgagatt
     1981 cagcccgccg gcggcgttgg agaccacgag cgccttcgcg ccgagctccc ggagaacgcg
     2041 caccgcgaac gtcacctctt cgagcgagta gccttcgtaa aaatggaatc tcccttccat
     2101 cgccgcgact cttttgccgc cgatctcccc gagcacaagc tgtcccttgt gagactgcac
     2161 ggtcgaacgc gggaaatgag gcaatttctc gtacgggaac acccgcacgg tcctgatcct
     2221 gtcagcaagc tgtccgagcc cggtgccaag gatcaatgcg atctcgggcc tgaagtcggt
     2281 ctgcctgcga atgaatttca cacagtcacc gatatgtttc atcagaccgc cctttggctg
     2341 acagaaccgc ttcatcgcca tctcacattc ctccctggat tttataagcc accgacaaaa
     2401 gcagtgtgac aagcacccga ttcacaaaaa caagcaaaat gaaagccagg atcggcgaga
     2461 aatccatcgg gccgatcctg atcggaatct tccggagcgg ttcaaggatc ggatcggtca
     2521 cctgcataag gaactggacc acgctggaat aagggtccac gggaaaccag gaaatgatga
     2581 tccgcgcgaa cagcatccag tagaggatcg tcgtgatccc gttgacgagc atcgcaagcg
     2641 ccttgaatat ttccgcaaaa atgaacatct cttttccttt tcctcgtctt caaaatcggg
     2701 gagtgacccc gctctatttt gcccgaagct ggcggctgcg tcgcgccgcg gccgcaagag
     2761 cggattggaa gattttt
//
LOCUS       DAOOBX010000107         2756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_40650_length_2756_cov_3.615698, whole genome shotgun sequence.
ACCESSION   DAOOBX010000107 DAOOBX010000000
VERSION     DAOOBX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40650_length_2756_cov_3.615698"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLY30_02165"
     CDS             complement(<1..215)
                     /locus_tag="PLY30_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW64939.1"
                     /translation="MVIIPEKEVRMEPKIRVGVVGVGHLGKEHARIYSQLSECQLVGI
                     SDRDPAKADKARELGTAYYRDYKDLLG"
     gene            488..2239
                     /locus_tag="PLY30_02170"
     CDS             488..2239
                     /locus_tag="PLY30_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW64940.1"
                     /translation="MDIYKRLLAYVKPHRWRLFVAVLSMFAYSIANSLVSATLYIIVN
                     GLYNKDEVRLDNLPHIPFLSSISFPVFWIPFVMIGVFFLRGFFDYLSNYQMSNIGIRA
                     IRQVRDDLYRHLVYLSHDFYSRGRTGDFLSRIMNDVGQIQGAITDVIVDLVKQPLVIL
                     FNIPWVFIWGGKYALFAILIFPLVAVPITILGKRLRKLTKSMQERTGDITSFIGETLS
                     GFHIVKAFCREEKEISRFEEINRSVFEYFRKTIKVTIIQRPLIEVMGAIGAGIAVGFG
                     IKYLPPDRFVAFVGSLFIFYEPLKKISKVNSTIQQSIAAGQRIFEVLDARPSIRNKPG
                     ALVFKEPVHEVAYENVSFEYEKGTPILQGIDIRAKQNEVIAIVGASGSGKTTLVSLLL
                     RFYDPTEGIIRINGKDIRELSLESLRGMIGIVSQETVLFNCSVKDNIAYGRPEAAIED
                     VRKAAMIAYADHFIEALPQGYDTQLGERGLKLSGGERQRLAIARALIKDPPILILDEA
                     TSHLDTQSEKEVQKALENLMKNRTVFVIAHRLSTVQKASRILVMEEGKIVQQGTNEFL
                     LRLGGVYKKLYDLQFNL"
     gene            2273..>2756
                     /locus_tag="PLY30_02175"
     CDS             2273..>2756
                     /locus_tag="PLY30_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64941.1"
                     /translation="MDIPKAEEPTPHPEETPENPGNRPGPDELVAAVKQLAERLETLE
                     KKAAELSGEVRSLGSEVKRVLAGLRRETKGSSGDVSAKEPSSKQGERGPGGRRRRRER
                     TTFKTVCAACGKECEIPFKPSSDRAVYCDACFSAHKPGKKTRAREMRETVPLRDDIAK
                     V"
BASE COUNT          595 a          785 c          772 g          604 t
ORIGIN      
        1 ttcccgagga ggtctttgta gtcccggtag taggccgttc cgagctcccg ggccttgtcg
       61 gccttggccg ggtcccggtc ggagattcca accagctgac attcagaaag ctgactgtaa
      121 atcctggcgt gttccttacc gagatgtccg acgccaacga ctccaaccct gatctttggc
      181 tccatcctga cctctttttc tggaataata accatatcca acctcgtagg ttggatatgg
      241 ttatgaaagc atgaccgtga accggggtac ccccacgaag gtgtttcagg agcgaccatt
      301 atatagcgga aggcggggga gtgaccaaga ttatttccgg ggaagatgac gggaattttt
      361 gatggtacgg ggtcaatgaa tatgttacat tgtggcctct ttctgtcgca aagacacaac
      421 cccatgccat gcgatcttct catgcgcctt tgtggtctgg catgacatgt ggtttcaaag
      481 gagttccatg gacatctaca agcggttgtt ggcctatgtc aagcctcacc ggtggcgcct
      541 tttcgtggcc gtcctgtcga tgtttgccta ttcgatcgcc aattccctgg tgtcggcgac
      601 gctctacatc atcgtgaacg gcctttataa caaggacgag gtccggctcg ataacctgcc
      661 gcacatcccg ttcctttcct cgatctcgtt cccggtcttc tggataccgt tcgtgatgat
      721 cggagtcttt ttcctccgcg ggttcttcga ttacctctcg aactatcaga tgtcgaacat
      781 cggaattcgc gcgatccgtc aggtccgcga cgacctctat cgccaccttg tttatctttc
      841 gcacgatttc tattcccggg gccgcaccgg cgacttcctt tcccggatca tgaacgatgt
      901 cggccagatc cagggcgcca tcacggatgt catcgttgat ctcgtgaaac agccgctcgt
      961 catccttttt aatatcccgt gggtcttcat ctggggaggc aaatacgccc ttttcgcgat
     1021 cctgatcttc ccgctggtcg cggtcccgat cacgatcctc gggaaacgcc tgcgcaagct
     1081 cacgaaatcg atgcaagaac ggacgggcga catcacctcc tttatcggcg agacgctttc
     1141 ggggttccat atcgtgaagg ccttctgtcg cgaagagaag gaaatctcgc gtttcgaaga
     1201 gatcaaccgg agcgtcttcg aatatttccg caagacgatc aaggtcacga tcatccagcg
     1261 tcccctgatc gaggtcatgg gcgcgatcgg cgccggcatc gcagtggggt tcggcatcaa
     1321 gtatcttccg ccggaccgct ttgtcgcgtt cgtcgggtcg cttttcattt tctacgagcc
     1381 cctcaagaag atcagcaagg tcaacagcac gatccagcag tccattgcgg cggggcagcg
     1441 tattttcgag gtcctcgatg ccaggccgag catccggaac aagcccggcg ctcttgtctt
     1501 caaggaacct gtccacgagg tggcctatga gaacgtcagc tttgaatacg aaaaaggcac
     1561 gccgatcctc caggggattg acatccgcgc gaagcagaac gaagtgatcg cgatcgtcgg
     1621 cgcgagcggc tccggcaaga caacgctcgt gagcctgctc ctgcgttttt atgacccgac
     1681 cgaggggatc atccggatca acgggaagga catccgggaa ctgagcctcg aatcgcttcg
     1741 cggcatgatc gggatcgttt cccaggaaac ggtccttttc aattgctcgg tgaaggacaa
     1801 tatcgcctac ggccgtccgg aggcggccat cgaggatgtc agaaaagccg cgatgatcgc
     1861 ctacgctgat catttcatcg aagcgctccc gcagggttac gacacccagc ttggcgagcg
     1921 cggcctcaaa ctttcgggcg gagagcggca gcgccttgcg atcgcccgcg ccctgatcaa
     1981 ggaccccccc attctcatcc ttgacgaggc gacttcacat ctggacacgc agagcgaaaa
     2041 agaggtccag aaggcgcttg agaacctcat gaagaaccgc accgtgttcg tgatcgcgca
     2101 ccgtctttcg acggtccaga aagcgagccg gattctggtg atggaggaag gcaagatcgt
     2161 ccagcagggg acgaacgagt tccttctccg tctcggcggt gtttacaaga aactctacga
     2221 tcttcagttt aatctctgag atcaaaaatc cgttttacgg atcaggagac ctatggatat
     2281 tccgaaagca gaagaaccga ccccccatcc cgaagaaact ccggaaaacc ccggaaaccg
     2341 tcccgggccc gatgaacttg tggccgcggt gaaacaactg gccgaacggc tggaaaccct
     2401 tgagaagaaa gctgctgaac tctcggggga agtgaggtcc ctcggaagtg aagtgaaacg
     2461 ggtccttgcc ggtctccgac gtgagacgaa aggttcctcg ggagacgttt ccgcgaaaga
     2521 accttcctca aaacaggggg agagagggcc gggaggccga cgccgtcgcc gggaacgtac
     2581 gacgttcaag acggtgtgcg cggcatgcgg gaaagaatgc gagattccct ttaagccctc
     2641 ttccgaccgc gcggtctatt gcgacgcctg tttttccgct cacaagccgg ggaaaaagac
     2701 gcgtgcccgg gagatgcggg agacggtccc gcttcgggat gatatcgcca aggtgg
//
LOCUS       DAOOBX010000108         2743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_40925_length_2743_cov_3.578125, whole genome shotgun sequence.
ACCESSION   DAOOBX010000108 DAOOBX010000000
VERSION     DAOOBX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40925_length_2743_cov_3.578125"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLY30_02180"
     CDS             <1..449
                     /locus_tag="PLY30_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW64942.1"
                     /translation="TVEENIMAILETLEISQDERDRRLKRLLNDLDIAYLAKNKAYTL
                     SGGERRRLEITRALVTNPSLILLDEPFSGVDPIAVFEVQKILQKLKAQGLSILLTDHS
                     VRETLSITDRSYLLYEGKVEVSGTSEFLANDAKARELYLGERFSLA"
     gene            490..1044
                     /gene="raiA"
                     /locus_tag="PLY30_02185"
     CDS             490..1044
                     /gene="raiA"
                     /locus_tag="PLY30_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPW64943.1"
                     /translation="MDIRFTGQNLKITTGMKEHIEGKLPKFEKYVPRIVEAHVCLKKE
                     KYFFVAEITVLAKGLHAFGAGRSKENIFASMDNAYDRVLKQLKKHCARKKDHYQKGGK
                     STLRAASRGEELVKAEAALGSRRPKIVGTSDFAVKPMSPEEASLQLEISKESFLVFLN
                     SSTNRVNVLYQRKDGDHGLIEPQF"
     gene            1076..1540
                     /locus_tag="PLY30_02190"
     CDS             1076..1540
                     /locus_tag="PLY30_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPW64944.1"
                     /translation="MKTTDFLSVKGIKLDLEATDKEGVLQEMVDLLAEVKSVGDKKAI
                     LKALIERENLGSTGIGQGIAIPHGKTDKVDGLFAVLGVSKKGVNFEALDGEPVYILFL
                     LVAPKDTAGPHLKALAQISRLLRDSYFCELLRRCKTPKEVFDLIAKEESRKV"
     gene            1564..>2743
                     /locus_tag="PLY30_02195"
     CDS             1564..>2743
                     /locus_tag="PLY30_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HPW64945.1"
                     /translation="MQFFKWFYPGIGIKRWIMLCVIGLTLLVVVALLTVKTIAKSSVM
                     LAAFATAILIFGIFLIYTSIKHMVRIFVRALMPLHQNGPLVDIVYQSRQSESLLRGPR
                     VVAIGGGTGLSTMLSGIKQFTSNITAIVTVTDTGGSSGRLRDEMDVLPPGDIRNCLVA
                     LADAGPLIRNLFQFRFKTGEGLKGHSFGNLFITALSMVTGDFEKAIAESSKVLAIRGR
                     VLPSTLEKVTLVGEFMDGTSREGETNITDERKPLRSIHLRPDNCAACPEALDALSTAD
                     LIIMGPGSLYTSILPNLLIEEIRNAILNSDAYKVYICNAMTQPGETAGFSAWDHLNVI
                     IEHTDPRIVDAVFVNTGRIPEPVFNRYREAGALPVKLDLDTIRDKGYEVVTDDVLKVD
                     G"
BASE COUNT          603 a          775 c          802 g          563 t
ORIGIN      
        1 tcacggtgga agagaatatc atggcgatcc tcgaaacgct tgagatctcg caggacgaga
       61 gggaccgccg gctgaaacgc cttctgaacg acctcgatat cgcttatctc gcgaagaaca
      121 aggcctacac cctttcgggc ggcgagcggc gccgtctcga aatcacgcgg gccctcgtaa
      181 cgaacccctc gctcatcctt ctcgacgaac cattcagcgg tgtcgatccg atcgccgtat
      241 tcgaagttca gaagatcctc cagaagctca aggcgcaggg actcagcatt ctgttgaccg
      301 accacagcgt gcgggaaacc ctctcgatca cggaccgttc ctacctgctt tacgagggca
      361 aggtcgaggt gtcagggacc tcggagtttc tcgcgaacga cgcgaaagcc cgtgaactct
      421 acctcggcga aaggttttcg ctcgcgtaac ttttttactg ttccttttta ccattcaagg
      481 aggcaatcga tggatatccg ttttacgggc cagaatctca agatcacgac gggcatgaag
      541 gagcacatcg aaggaaagct ccccaagttc gagaaatacg tcccccgcat cgtggaagcg
      601 catgtgtgcc tcaaaaaaga gaaatacttt tttgtggccg agatcacggt tctcgcgaag
      661 gggcttcacg cgttcggcgc gggccgttcc aaggagaaca tcttcgcgtc gatggataac
      721 gcttatgacc gcgtccttaa acagctcaag aaacactgcg cccggaaaaa agaccattac
      781 cagaagggcg gcaagagcac gctgcgcgcc gcgtcccgcg gggaagagtt ggtcaaggcg
      841 gaggccgcgc tcggcagccg gcggccgaag attgtcggga cgtcggattt tgccgtgaaa
      901 ccgatgtctc ccgaggaagc gagccttcag ctcgaaatct ccaaagagtc ttttctggtg
      961 ttcctgaatt ccagcacgaa ccgcgtgaac gtcctttatc agcgaaagga cggggatcac
     1021 gggcttatcg aaccgcaatt ctaatccacc actctcacgt gaggagggag tcagtatgaa
     1081 aacaacggat tttcttagcg tcaaaggcat caagctggac cttgaagcca cggacaagga
     1141 gggcgtcctg caggaaatgg tcgaccttct tgccgaggtc aagagtgtcg gcgataaaaa
     1201 ggccattctg aaggcgctga tcgagcgcga gaatctcggg tctacgggga tcgggcaggg
     1261 catcgccatc ccgcatggaa agaccgacaa ggtcgacgga cttttcgcgg tgctcggtgt
     1321 cagcaaaaag ggcgtgaatt ttgaggcgct cgacggggag ccggtttaca tcctgtttct
     1381 ccttgtagcc ccgaaggata ccgcgggccc gcacctcaag gccctcgcgc agatctcccg
     1441 gctgctccgc gactcctatt tctgcgagct cctgcgccgc tgcaagaccc cgaaggaggt
     1501 ttttgacctg atcgccaagg aagagagccg caaggtttaa ggcccgggaa aggcgcatcg
     1561 cgaatgcagt tcttcaagtg gttctatccc ggcatcggca tcaaacgctg gatcatgctc
     1621 tgcgtgatcg gcctgacgct gcttgtggtg gtcgcactcc tcacggtgaa gaccatcgcg
     1681 aagtccagcg tcatgcttgc cgctttcgcg acggcgatcc ttattttcgg aatcttcctg
     1741 atctatacgt cgatcaagca catggtccgt atttttgtgc gcgcccttat gccgctccac
     1801 cagaacggac ctctcgtgga tatcgtttat cagagccggc agtccgagtc gctgctgcgc
     1861 gggccgcggg tcgtggcgat cggcggcgga acgggcctga gcacgatgct ttccggtatc
     1921 aaacagttca caagcaacat cactgcgatc gtgaccgtca ctgataccgg cgggagttcg
     1981 gggcgcctgc gcgatgagat ggacgttctg cccccgggcg atatccgcaa ctgcctggtg
     2041 gcgctcgcgg acgcggggcc gctgatccgc aatcttttcc agtttcgttt caaaacggga
     2101 gaaggtctga aagggcacag tttcggcaat cttttcatca cggccctttc catggtcacg
     2161 ggggatttcg aaaaagcgat cgccgagtca agcaaagtcc tcgcgatccg ggggcgggtg
     2221 cttccctcca cgctcgaaaa agtgacgctt gtgggtgaat ttatggatgg tacgtccagg
     2281 gagggtgaga ccaatatcac ggacgaacgc aagccgctcc ggagcattca tttgcgtccg
     2341 gacaattgcg ccgcgtgccc ggaagccctt gacgcgcttt cgaccgcgga cctcatcatc
     2401 atgggccccg gaagtcttta caccagcatc ctgccgaatc tcctgatcga agagatccgg
     2461 aatgcgatcc tgaattcgga tgcctacaag gtctatatct gcaacgccat gacgcaaccg
     2521 ggcgaaacgg cgggtttcag cgcgtgggac cacctgaatg tcatcatcga acatacggat
     2581 ccccggatcg tggacgcggt cttcgtcaat acaggccgga tcccggagcc ggtcttcaac
     2641 cgttatcgtg aggcgggagc gttgccggtg aaactggatc tcgatacgat ccgtgataag
     2701 gggtatgagg tggtgaccga tgatgtcctg aaggtcgacg gac
//
LOCUS       DAOOBX010000109         2723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_41307_length_2723_cov_3.125562, whole genome shotgun sequence.
ACCESSION   DAOOBX010000109 DAOOBX010000000
VERSION     DAOOBX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41307_length_2723_cov_3.125562"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1210)
                     /gene="mraY"
                     /locus_tag="PLY30_02200"
     CDS             complement(125..1210)
                     /gene="mraY"
                     /locus_tag="PLY30_02200"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPW64946.1"
                     /translation="MFYFFHHLTDLSIVFNLFRYITFRSAGASITAFFLCLWFGPSCI
                     AWLKRLKAVTDQKRAHAEAIHEFYKNKKDTPMMGGILIVASVIASVLLWGNLTNRFVW
                     LMVVVTLWFGFVGCFDDWLKMRQGNASGLNSLAKLSGQVLVGLAIGVYLYFDPSFSKW
                     LYVPLLKKAVLYLGIFFIPFVILVLAGSSNALNLTDGLDGLAIGCTMFTTTAFAVIAY
                     VTGHADFASYLQIQYLPEAGELTVFCAALAGASAGFLWFNSYPAEVFMGDTGSLSIGG
                     AIGTIAVLTKKEIVLVIIGGVFVWEALSVILQVASLKLAKKRIFLMSPFHHHLQRKGW
                     PESKITVRLWIIAFILAIVGLSSLKVR"
     gene            complement(1226..2632)
                     /gene="murF"
                     /locus_tag="PLY30_02205"
     CDS             complement(1226..2632)
                     /gene="murF"
                     /locus_tag="PLY30_02205"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPW64947.1"
                     /translation="MLPYPEAGRALGVPVEYSDAVPEIRSFSLDTRTLKPGELFIALK
                     GERSDGHAHVPAAYAAGASGVLIDRERKSDVLALLKNAGRVATNLLAVPEPERAMTDL
                     ARYYRGQLSLRAVSVTGSFGKTTTKEFLVFLLRQKYSALATSGNLNNHLGVPIMISRL
                     ERSHNVAVFELGASGMGEISHLAGLIRPSAAVITMIGPAHLEGFGSLENIYRAKTEVV
                     RHLPKNAPVVISDQDPELERVLRDSGKRIVRAGPSEGADCRLTDVRAADGRVSFRLNG
                     VGPFSFPGHAPFLGMNAALAAAMACEWGVPLADMPRDWRDAGFATGRFRETTLANGIR
                     VIDDSYNASPLAFAAAVTTFSKLPATGKRILVFADMLELGAEELRYHRELGERISELP
                     IDLAVAFGERARASIEAIRAAGVPVSARHFPDAETLSATLAEIVRPGDLVLFKGSRGM
                     RVERVLTRIKELVFSTSH"
BASE COUNT          657 a          841 c          725 g          500 t
ORIGIN      
        1 acatcttcct cctttgaaag atcggacgcg aaaacacgat agccgcgccc ggccaggcaa
       61 agggcgctat gataaccgga atccttcaga ccgagaatcg cgacgttctt ttttgtttcc
      121 ataatcagcg caccttgagc gaactcagac cgacaatcgc cagaataaag gcgataatcc
      181 agaggcgaac cgtgatcttg gattcgggcc atcccttgcg ttgcagatga tggtgaaaag
      241 gagacatcag gaagatccgc tttttcgcaa gcttgaggct ggcgacctga aggatcacgc
      301 tcagagcctc ccagacgaac acaccgccga ttatcacaag cacgatctct ttttttgtga
      361 gcacggcgat cgtcccgatc gcgcccccga tcgaaagcga accggtatcc cccatgaaaa
      421 cctcggccgg atacgaattg aaccagagga atcccgcgct cgcgcccgcg agcgccgcac
      481 aaaagaccgt gagttccccg gcctccggca ggtattgaat ctggagatac gacgcgaaat
      541 ccgcatgtcc cgtcacatac gcgataaccg cgaacgccgt cgtggtgaac atcgtgcagc
      601 cgatcgcgag tccgtccaga ccatcggtca ggttcagcgc gttcgaggat ccggcgagca
      661 caaggatcac aaaaggaata aaaaagattc ccagatagag caccgccttc ttgagcagcg
      721 gaacatacag ccatttgctg aaggacggat cgaaataaag atagacaccg attgcgagcc
      781 ccaccaggac ctgaccggac aatttggcga gcgaattaag cccgctggca ttcccttgcc
      841 gcatcttcag ccagtcatcg aagcaaccca cgaaaccgaa ccagagcgtg acgacaacca
      901 tcagccacac gaaccggttc gtcagatttc cccacagcag gacagacgcg atcacgctcg
      961 cgacaatcag gatgcccccc atcatgggcg tatccttttt gttcttgtaa aactcgtgga
     1021 tcgcctcggc atgcgcgcgt ttctgatcgg tcacggcctt caaacgcttt aaccaggcga
     1081 tacaggacgg cccgaaccag aggcaaagga aaaacgccgt gatgcttgcg cccgcggaac
     1141 ggaaagtgat gtaacggaac aggttaaaaa caatcgacag gtcggtcaga tgatgaaaga
     1201 aatagaacat ggctctcttt atcggctaat ggcttgtact gaaaacaagt tctttgatcc
     1261 gggtcagcac cctctcgaca cgcatcccgc gcgatccttt aaacagaacg aggtcgcccg
     1321 gtctaacgat ctccgcaagc gttgccgaaa gggtctctgc gtccggaaaa tgtctggcac
     1381 tcacaggaac acccgcggcc cgtatcgcct caatgctggc acgggccctt tcaccgaaag
     1441 caaccgcgag atcgatcgga agttccgaga ttctttcgcc aagttcccgg tgatagcgga
     1501 gttcttccgc ccccagctcc agcatatcgg caaaaacaag aatccgttta ccggtcgcgg
     1561 ggagcttcga gaatgtcgta accgccgccg cgaacgcgag cggactcgcg ttgtaactat
     1621 catcgattac ccggataccg ttcgcgagcg tcgtctcacg gaaacgaccc gtcgcgaaac
     1681 cggcgtcccg ccaatcgcgg ggcatatccg cgagcgggac cccccactcg caggccatcg
     1741 cggccgcaag cgccgcgttc atgcccagga acggtgcgtg ccccggaaac gaaaaaggcc
     1801 cgacaccatt cagcctgaaa gaaacccgcc cgtccgccgc cctgacatcg gtcagtcggc
     1861 agtcagcacc ctcggaggga cccgcgcgca cgatgcgctt gccgctgtct ctcaggaccc
     1921 gttcgagttc cgggtcctgg tcggagatca cgaccggggc gttcttcggg agatgccgga
     1981 caacctcggt ctttgcgcgg taaatgtttt caaggctgcc gaaaccctcc agatgcgccg
     2041 gcccgatcat tgtgatcact gcggccgacg gccggatcag accggccaga tgagaaatct
     2101 cacccatacc gctcgcgccc agctcaaaga ccgcaacatt atgggaacgc tccagacgcg
     2161 agatcatgat cggaacgccg agatgattat tgagattccc cgatgtcgcg agcgcggaat
     2221 atttctggcg taaaagaaaa acaaggaact ccttggtcgt cgttttaccg aagcttccgg
     2281 taacactaac cgctctgagc gaaagctgtc cccggtaata gcgcgcaaga tccgtcatcg
     2341 cccgctcggg ttccggcacc gcgagaaggt tcgtcgcaac gcgcccggca ttttttaaca
     2401 acgccaggac atccgatttc cgttcccgat cgatcagaac tccggatgcg cccgccgcat
     2461 acgccgcagg aacgtgggca tgcccgtcgg atcgttcgcc ctttagcgcg ataaagagtt
     2521 cacccggctt cagggtgcgg gtatctaagg aaaaactcct gatctcgggg accgcatcgg
     2581 aatattccac cgggacacca agcgcccggc cggcttcagg ataaggcaac acggcttttt
     2641 cggatggagg cctcgaccgt cgcgcggtcg ctgaacggga tctttttgtc gccgagaatc
     2701 tgataatcct catgcccttt acc
//
LOCUS       DAOOBX010000110         2714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_41496_length_2714_cov_4.494547, whole genome shotgun sequence.
ACCESSION   DAOOBX010000110 DAOOBX010000000
VERSION     DAOOBX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41496_length_2714_cov_4.494547"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /gene="uvrA"
                     /locus_tag="PLY30_02210"
     CDS             complement(<1..2036)
                     /gene="uvrA"
                     /locus_tag="PLY30_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPW64948.1"
                     /translation="MSADQEYLEVRGAREHNLKNLSVRIPRNKFTVITGLSGSGKSSL
                     AFDTIYAEGQRRYLESLSSYARQFLEKLKKPDVDHISGLSPSISIEQRSATHNPRSTV
                     ATVTEIYDYLRLLFSKVGTAHCPSCGLPMRSQTRDELARDILVRFGGHEVQVLAPLVR
                     GRKGEYQKLFQDMLKKGYDRARIDGTERLLAPEMKLKKTVRHDIGIIVDELVLKENKE
                     DKRFRNSLACALDLAGGQVAILDRDVAGQALHYFSTTKNCLSCQISLPELTPNMFSFN
                     SPYGACPKCKGLGKLSQVFSKGVIADPKKALMQALNKEVFFSFNKYFFEDLLHDLSHK
                     YDFEWDTPYGDWPETAKEAFFLGDGDIAGLVEEWERLFHETDSDMIRAKIRKFLREET
                     CKECGGKRLRKESLGVSVAGKNIAEVTSLLVEDAAPYFEALSFSGNDAVIAAPILHEI
                     RERLAFLLNIGLGYLTLDRTVATLAGGELQRIRLAAQISLGLTGVLYVLDEPSIGLHP
                     RDNEKLLDALERLRDLKNTVLVVEHDEETIRRADHVIDLGPGSGAKGGEIVAQGAISE
                     MKDCPRSVTLKYLHGELSINVPEKRKDYRNAPKLLLKKCAEHNLKEIDVEIPLGLFTC
                     VTGVSGSGKSTLVHDILYRELHNRLWKTGYKAGDFGAIEGAELIDRVIGIDQTP"
     gene            complement(2045..>2714)
                     /locus_tag="PLY30_02215"
     CDS             complement(2045..>2714)
                     /locus_tag="PLY30_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF108 domain-containing protein"
                     /protein_id="HPW64949.1"
                     /translation="RKLPIVSIPEIIKRCDFVIEAAHSSISSSVASLALKHDKYVLVM
                     SVGGLLKSPFGRLRALIAKSSGRLYCPSGAIAGVDALLAAQTNGLRRVKLVTTKPPQA
                     LKGAPYFKTHAFPVFKSGEGKKLVFRGTALQASRAFPQNINVAAILSLAGLGPKRTEV
                     EIWATQMAGTNRHEVLIEGSFGTIRTTTDNFPAPDNPKTSYLAILSAAATLRKAFTTL
                     RLGT"
BASE COUNT          573 a          796 c          760 g          585 t
ORIGIN      
        1 atgggagtct gatcgatccc gatcaccctg tcgatcagct ccgcgccctc gatcgctccg
       61 aagtccccgg ccttataccc ggtcttccag agccggttgt ggagttccct gtagaggata
      121 tcgtgcacca gcgtgctttt tcccgacccc gaaacacccg tgacgcatgt aaaaagcccc
      181 aggggaatct caacatcgat ttccttgaga ttgtgctcgg cgcatttttt cagcagcagt
      241 ttcggcgcgt tccggtagtc ttttcttttt tccggaacgt tgatcgacag ttctccgtga
      301 agatacttga gtgtgacgga ccgcgggcaa tctttcatct ccgatatcgc cccctgcgca
      361 acgatctccc cgcctttcgc cccggagccg gggccgaggt cgatcacgtg atcggcgcga
      421 cggatggtct cctcgtcatg ctccacgaca aggaccgtat ttttcagatc gcgaagacgc
      481 tcaagggcat ccagcaactt ttcgttatcg cgcgggtgaa ggccgatgct cggctcgtcc
      541 agaacgtaca gcacccccgt cagcccgagg ctgatctgcg cggcaaggcg gatgcgctga
      601 agctcgcccc cggcaagcgt tgcgaccgtg cggtcaagcg tgaggtagcc aagcccgata
      661 ttcaggagga acgccaggcg ttcccggatc tcgtgaagga tgggagccgc gattaccgcg
      721 tcgttcccgg aaaaagaaag cgcctcgaaa tagggcgccg catcctcaac gagcagggac
      781 gtcacctcgg caatattctt tccggcgacc gaaacaccga gactttcctt ccggagcctc
      841 ttcccgccac attccttgca ggtctcttcg cgcaggaatt ttcggatctt ggcgcggatc
      901 atatcggaat ccgtttcgtg aaaaagacgc tcccactctt ccacaagccc cgcaatatcc
      961 ccatcaccga gaaagaacgc ctcttttgcg gtctccggcc aatccccgta cggagtatcc
     1021 cattcaaaat catatttatg ggagagatcg tgcaaaaggt cctcaaagaa atacttgtta
     1081 aacgaaaaga acacctcctt gttcagggcc tgcatgagag ctttctttgg gtccgcgatc
     1141 acacccttcg agaagacctg agaaagtttc ccgagcccct tgcatttcgg acaggccccg
     1201 taggggctat tgaacgagaa catgttcggt gtgagttccg gaaggctgat ctggcaggac
     1261 agacagtttt tcgtcgttga gaaataatga agcgcctgac ccgcgacatc cctgtcgagg
     1321 atcgcaacct ggccccccgc gagatcaagg gcgcaagcga gcgaattccg gaaacgcttg
     1381 tcctccttat tctccttcag gaccaattcg tcaacgatga tcccgatgtc atgacggacc
     1441 gtttttttga gcttcatctc gggcgcgagc aaccgctccg tcccgtcgat ccgggcacgg
     1501 tcatagccct tcttcagcat atcctggaac agtttctgat attcgccttt tctgccccgc
     1561 acgagcggcg ccagcacctg cacctcgtgc ccgccgaatc gcacgaggat gtcgcgagca
     1621 agttcatctc gggtctggct ccgcattggt aatccgcagg aagggcaatg cgcggtgcca
     1681 actttcgaaa agaggaggcg gagataatca tagatctcgg tcacggtcgc gaccgtggaa
     1741 cgcggattat gggtcgcgga gcgctgttcg atggaaatgc tcggagaaag acctgagata
     1801 tgatcgacat ccggcttctt cagtttttcg aggaattggc gggcatacga cgagaggctc
     1861 tcaagatagc gacgttgacc ttcggcatag atcgtgtcga acgcgagcga ggattttccg
     1921 gagcctgaga gcccggtgat gaccgtgaac ttgttgcggg ggatccggac cgagagattc
     1981 ttgaggttat gctccctcgc cccacggacc tcaaggtact cctgatccgc gctcataccc
     2041 cttatcaggt gccaagccgc agggtggtga aggctttccg aagggtcgcc gctgcggaga
     2101 gaatggcaag atagctcgtt ttgggattat caggggcggg gaaattatcg gtcgtcgtgc
     2161 ggatcgttcc gaagctcccc tcgatcagga cctcgtgccg gttagtcccc gccatctgcg
     2221 tggcccagat ctcaacctcg gtcctcttgg ggccgaggcc cgcaaggctc aggatcgcgg
     2281 ccacgttgat attctgcggg aatgcgcggc tcgcctgaag cgccgtaccg cgaaaaacca
     2341 gtttttttcc ctcgccggat ttgaagaccg ggaaagcgtg agtcttgaaa tacggcgccc
     2401 ctttcagcgc ctgcggcggc tttgtggtca caagtttcac gcgccgaagg ccgttcgtct
     2461 gcgcggccaa caaggcatcg actcctgcga tcgccccgga cggacaataa agacgccctg
     2521 agcttttcgc gatgagggcg cgaagcctgc cgaacgggga cttcaaaagt cccccgacgc
     2581 tcatcacaag aacgtatttg tcatgcttga gggcaagcga ggcgacggaa gaagaaatac
     2641 tcgaatgcgc ggcttcgata acaaaatcgc agcgtttgat gatctccgga atggacacga
     2701 tcggaagttt cctg
//
LOCUS       DAOOBX010000111         2685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_42094_length_2685_cov_3.293156, whole genome shotgun sequence.
ACCESSION   DAOOBX010000111 DAOOBX010000000
VERSION     DAOOBX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42094_length_2685_cov_3.293156"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..933
                     /locus_tag="PLY30_02220"
     CDS             271..933
                     /locus_tag="PLY30_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPW64950.1"
                     /translation="MGADVFCLTTNGLKMALEGEGFMDGIIYILINAAMPGYVKIGRT
                     TNLEKRIRDLDTTNIPLPFECFYACTVKNANFVEKQLHDAFSDRRVRFSREFFEIAPE
                     RALAALRLAEVENVTPKKDYVETEEDRQALNQARAKRAIFNFKMVDIPPGAELFFIND
                     ENIKAKVIDNRSVEYNGEVTSLSNAAQKALGREYSVQGPIYWMYEGETLEERRRRFEA
                     GE"
     gene            937..2433
                     /gene="gatB"
                     /locus_tag="PLY30_02225"
     CDS             937..2433
                     /gene="gatB"
                     /locus_tag="PLY30_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPW64951.1"
                     /translation="MNYEPVIGLEVHVQLKTKSKLFCPCPTTFGEEGNRNTCPVCLGW
                     PGSLPVVNEEALRLGIKAGLALNCKVAERLKFDRKNYFYPDLPKAYQISQYDMPVNGR
                     GEVLIETKTPDGKTVEKKIGITRAHLEEDAGKLLHEGIVDGSLVDYNRGGVPLLEIVS
                     EPDIRSPQEAYDYLTALKAILRYAEVSDCDMEKGSLRCDANVSVRPVGQEKFGTKVEI
                     KNLNSFKMVQRAIQCEIARQIEAITDGETIVQETRLWNDAKGMTFSMRSKEDAHDYRY
                     FPDPDLVPFTLTRETVEAIRKTLPELPLERAKRFMRDHGLPEYDACTLVEDKKVADYF
                     EECLKGRERGQTPFSEDREKVSDPFSAKRASNWILTELLALLNAKGLTVENCPVAPKA
                     FAGLLNLIEGGTISGKIAKDVLGMMFETGKDAETLVKEKGLVQVSDAGLIEEAVEKAI
                     AANPNAVAEFKAGKQKAVGAIVGFVMKETGGKANPKVVNEILCRKLKN"
     gene            2412..>2685
                     /locus_tag="PLY30_02230"
     CDS             2412..>2685
                     /locus_tag="PLY30_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020754878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64952.1"
                     /translation="MSQAKKLVRRKGGAAGRGLRAPFYVLGIETSCDETSCAVIENGA
                     KILSNVVSSSLFRHRKFGGVVPEIASRHSMEQIRCVFDEALAEAKIR"
BASE COUNT          679 a          669 c          815 g          522 t
ORIGIN      
        1 agcaggaacc gtttcagccg cgcagattga aacggtggcg gaagcccggc ccggaagtga
       61 atttaaccgc atgcgggcaa attggaccgg gacggaattt cccggcccgc cggaaaaccg
      121 gacgaacggc agtgaatttt gtgtcaggag gggattaccg tcaaagaccg ggataccggt
      181 tatgatgggt gcacagcccg gcgaatccgc ggattatccg gcgtatgttc aaaaggtttt
      241 caaacagacg ggaaagcaaa tcggtattgt ttgggcgcgg atgtcttttg tttaacgaca
      301 aatggtttaa aaatggcgct tgagggagag ggctttatgg atggaataat ctatattctc
      361 atcaatgcag ccatgcccgg gtatgtaaaa atcggacgga cgaccaatct tgaaaagcgc
      421 attcgcgatc tggacacaac aaacataccg cttccgttcg aatgctttta tgcttgcacc
      481 gtcaaaaatg caaattttgt tgaaaagcag ttgcatgatg cattctcgga tcggcgcgtc
      541 cgtttcagca gagaattttt tgagattgct ccggagcgag ctcttgcggc actgcggctt
      601 gccgaagttg agaatgttac gcctaaaaaa gattatgttg aaacggaaga agatcggcag
      661 gccttaaatc aggctcgcgc aaagcgtgct atcttcaatt ttaaaatggt tgatatcccg
      721 ccgggggcgg agcttttctt cattaatgat gaaaacataa aagcgaaagt cattgataac
      781 cggtctgttg aatataatgg agaagttaca agcctttcaa acgcagccca aaaagccctt
      841 ggaagagagt actcagtgca ggggccgatt tattggatgt acgagggcga aacgcttgaa
      901 gaacgcagga gaaggtttga agcgggagaa tagaaaatga actacgaacc cgtaatcgga
      961 ttggaagtcc acgtgcagct gaagacaaag agcaagttgt tctgcccttg cccgacgacg
     1021 ttcggggaag aggggaaccg gaacacgtgc ccggtgtgtc tcggctggcc ggggtccctg
     1081 ccggtggtga atgaagaggc gctgaggctt ggcatcaagg cggggctcgc gctgaactgc
     1141 aaggtcgcgg agcggctcaa gtttgaccgc aagaactact tttatcccga tcttccgaag
     1201 gcgtatcaga tatcgcaata cgatatgccc gtgaacggcc gcggcgaagt cctgatcgaa
     1261 acgaaaactc cggacggaaa aacagtcgaa aagaaaatcg ggattacgcg cgcgcacctc
     1321 gaagaggacg cgggcaaact tctccacgaa ggcatcgtgg acgggagcct tgtcgattac
     1381 aaccggggcg gcgtgccgct tcttgagatc gtttccgaac ccgatatccg ttcgccgcag
     1441 gaggcctacg attacctgac cgcgctcaag gccattctcc ggtacgcgga agtgagcgac
     1501 tgcgacatgg aaaaaggaag cctccgctgc gacgcgaacg tgtcggtccg tcccgtcggt
     1561 caggaaaagt tcgggacgaa ggtcgagatc aagaacctga actcgttcaa gatggtccag
     1621 cgcgcgatcc agtgcgagat cgcacggcag atcgaagcga tcacggacgg cgaaacgatc
     1681 gtccaggaga cgcggctttg gaacgacgcg aaaggtatga ccttttccat gcgttcgaaa
     1741 gaggacgcgc atgactaccg ctatttcccc gacccggacc tcgtgccttt cacgctgacg
     1801 cgcgaaacgg tcgaagcgat ccggaagaca ctgcccgaat taccgcttga gcgcgcgaag
     1861 cggttcatga gggaccacgg tcttcccgaa tacgacgcct gcacgctcgt cgaggacaag
     1921 aaggtcgcgg actatttcga agaatgtctc aagggccgcg aaaggggtca gacacctttt
     1981 tcggaagaca gggaaaaggt gtctgacccc ttttccgcga aacgggcgag taactggatt
     2041 ctgacggaac tcctcgccct cctgaacgcg aaaggtctta cggtcgaaaa ttgtccggtg
     2101 gcgccgaaag cgttcgcggg tcttctgaat ctgatcgagg gcgggaccat ctccgggaag
     2161 atcgcgaagg acgtgctcgg gatgatgttc gagaccggca aggacgcgga aacgctcgtg
     2221 aaagaaaagg gcctcgtcca ggtctccgac gcggggctga tcgaagaggc cgtcgagaaa
     2281 gcgatcgccg cgaatccgaa cgccgtcgcc gagttcaagg ccgggaagca gaaggccgtc
     2341 ggcgcgatcg tgggcttcgt catgaaagag acgggaggca aggcgaatcc gaaggtcgtc
     2401 aacgagatct tatgtcgcaa gctaaaaaac tagtacggcg taagggcgga gcggccggtc
     2461 gcggactccg tgccccgttc tacgtactcg gtatcgaaac ctcctgcgac gaaacgtcct
     2521 gcgccgtcat cgaaaacggt gcgaagatcc tctccaatgt tgtttcctcg agtctcttcc
     2581 ggcacaggaa attcggcggc gtcgtgcccg agatcgcgtc gcggcattcg atggaacaga
     2641 tccgctgcgt ttttgacgag gcgctcgcgg aggcgaaaat caggg
//
LOCUS       DAOOBX010000112         2662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_42567_length_2662_cov_2.477177, whole genome shotgun sequence.
ACCESSION   DAOOBX010000112 DAOOBX010000000
VERSION     DAOOBX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42567_length_2662_cov_2.477177"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2494
                     /locus_tag="PLY30_02235"
     CDS             <1..2494
                     /locus_tag="PLY30_02235"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPW64953.1"
                     /translation="YGSVTFKQDPAPLLVGEQTNANGSKRFRQILERNDYEGLLEIGR
                     EAVKEGAHVIDLCVAFTGRDEKKDMTEAVTRFNQHVTAPLMIDSTEPAVLEEALKHIS
                     GKPIINSINLEDGEGKLATVCGLAKKYGAALVALVIDEKGMARTRDEKFAVAKRIHDL
                     AVIKHGIRPENIFFDLLTFTVGCGDENSRSAAIETLEAIKRVKKEFADVRTILGVSNV
                     SFGLAPQSRKILNSVFLHEAVAAGLDAAIVHVERIVPVFKIAPDDLEAAKNLIFNKWI
                     DGKDPLEVFIRHFETKTGSASAVTKPGGSDLPVEDRLKAHVIDGVKEGLEATLDEALK
                     KYRPFEIINQFLLEGMKVVGQLFGAGKMQLPFVLRSAEIMKRAVAYLEPFMEKKEGSE
                     RGRIVLATVKGDVHDIGKNLVDIILSNNGYKVFNIGIKQPIDAVLKAVEEHRADAIGL
                     SGLLVKSTHIMKEDLEEMNRRNITLPVLLGGAALTERYVCEDLASLYRGEVHYGRDAF
                     AALKIMEKIKAEDPETERIKEAKKMLAVKPVERPHAPRADHPREEPAKRRVSLVRHDN
                     IVPRPPFMGYRILKNLRLQDIFPWVNKKSLYTVQWQFKQGDRTERMFDLFLQENVEPL
                     FERFTQQCIREKILQPKAMYGYFPCYSEGDDLIVLDENGKIERTRFQFPRQKRDPYQC
                     IADFFRPKSSGLLDVVAFTVVTMGSRIREVEEKAFKGHNYQEYLYLHGLSVEATAALA
                     EYLHAMVRRELRIHLQDKQTKWEIFRQGYQGSRYSFGYPACPRLEDQRKLFGLIPADK
                     IGVSLSEIFQMVPEQSTSAVIVHHPQAKYFSV"
BASE COUNT          605 a          735 c          798 g          524 t
ORIGIN      
        1 ctacggctcg gtcactttta aacaggatcc ggcgccactt ctcgtgggtg aacagacgaa
       61 cgcgaacggc agcaaacgct tccgtcaaat cctcgaacgg aacgattacg aggggcttct
      121 tgagatcggc cgtgaggccg tgaaggaagg ggcccatgtg atcgatctct gcgtggcgtt
      181 cacgggccgc gatgagaaga aggatatgac cgaggcggtt acgcggttca accagcatgt
      241 gaccgcgccg ctcatgatcg attcgacgga gccggcggtc ctggaagagg ccctcaaaca
      301 catttccggg aagccaatca tcaattccat caatctggag gacggcgagg ggaagcttgc
      361 gacggtctgc gggctcgcaa aaaaatacgg ggccgcgctt gtggcgcttg tgatcgacga
      421 gaagggaatg gcgcggacgc gggatgagaa gttcgcggtc gcgaagcgga ttcacgatct
      481 tgccgtaatc aagcacggca tccgccccga gaacatcttt ttcgatctcc tgacgttcac
      541 ggtcggatgc ggcgatgaga attcgcggag tgccgcgatc gagaccctcg aagcgatcaa
      601 gcgggtcaaa aaagagttcg cggatgtccg cacgatcctc ggtgtcagca atgtctcgtt
      661 tggtctcgcc ccgcaatccc ggaagatcct gaacagcgtg ttcctccacg aagctgtggc
      721 ggcggggctt gacgcggcga tcgttcacgt cgagcgtatc gttcccgtct tcaagattgc
      781 tccggacgat cttgaagccg cgaagaacct tatctttaat aagtggatcg acggaaaaga
      841 cccgctcgag gtttttatcc gccactttga aacaaagacg gggagcgctt ccgctgtcac
      901 aaagccgggg ggtagcgatc ttccggtcga ggaccgtctc aaagcgcatg tgatcgatgg
      961 cgtgaaagag ggtcttgagg ccacgctgga tgaggccctc aaaaaataca ggccgttcga
     1021 gatcatcaat caattcctgc tggaggggat gaaggtggtc ggacagcttt tcggcgcggg
     1081 aaagatgcag cttccgttcg tgctccgatc ggccgagatc atgaagcggg cggtcgcgta
     1141 tcttgagccg ttcatggaaa agaaggaagg ttccgaacgc ggacgcatcg ttctcgcgac
     1201 cgtcaaaggg gacgtgcacg acatcgggaa gaacctggtc gatatcatcc tttcgaacaa
     1261 cggctacaag gtcttcaata tcgggatcaa acagccgatc gacgccgtcc tgaaggccgt
     1321 cgaagagcac agggccgacg cgatcgggct ttcggggctt ctcgtgaagt cgacccatat
     1381 catgaaggag gacctcgagg agatgaaccg ccggaatatc acgcttccgg tactcctggg
     1441 aggcgccgcg ctgaccgagc gctatgtctg cgaggacctt gccagccttt accggggcga
     1501 ggtccactac gggcgcgacg cgttcgcggc cctcaagatc atggaaaaga tcaaggccga
     1561 ggatcccgag accgaacgca tcaaggaagc gaaaaaaatg ctcgccgtga aaccggtcga
     1621 acgaccccat gctccccggg cggaccatcc ccgcgaagag ccggcgaagc ggcgtgtgag
     1681 ccttgtccgg cacgacaata tcgtcccgag acctccgttc atggggtacc ggatcctgaa
     1741 gaacctgcgc ctgcaggaca tcttcccctg ggttaacaaa aaatcactct acaccgtcca
     1801 gtggcagttc aaacaggggg atcgcaccga gcggatgttc gacttgttcc ttcaggagaa
     1861 tgtcgaaccc ctttttgagc ggttcaccca gcaatgtatc cgggagaaga tccttcagcc
     1921 gaaggccatg tacggctatt tcccgtgcta ttcggaaggg gacgacctta tcgtgctcga
     1981 cgaaaacgga aaaatcgagc ggacgcggtt ccagttcccg cgccagaaac gcgacccgta
     2041 ccagtgcatc gccgattttt ttcgtccgaa aagttccgga cttctcgatg tggtcgcctt
     2101 cacggtcgtg acgatgggat cgcggattcg ggaggtcgag gagaaagcct tcaagggaca
     2161 taactaccag gaatacctct acctgcacgg cctgtcggtc gaggcgaccg cggcccttgc
     2221 cgaatatctc cacgcgatgg ttcgaaggga attgagaatc cacctgcagg acaagcagac
     2281 gaagtgggag attttccggc agggctatca gggatcgcgg tacagctttg gttatcccgc
     2341 gtgtccgcgt ctcgaagacc agcggaagct tttcgggctc attcccgccg acaagatcgg
     2401 cgtaagcctt tccgagattt ttcagatggt gccggagcag tcaacctccg ccgtcattgt
     2461 ccatcacccg caggcgaaat acttctcagt ctgaaatccg tacggtgtgc ggcggccgga
     2521 gcacggagtg tgacaataaa aaagccggat gcgaactata acgcatccgg ctttttgctt
     2581 tgctcagtac gtcgcgctga atttaatgct tcgccgccgc aagtttttcc gcgagggaat
     2641 ggtattccgc ccagatcgct tc
//
LOCUS       DAOOBX010000113         2650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_42822_length_2650_cov_3.177649, whole genome shotgun sequence.
ACCESSION   DAOOBX010000113 DAOOBX010000000
VERSION     DAOOBX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42822_length_2650_cov_3.177649"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="PLY30_02240"
     CDS             <1..152
                     /locus_tag="PLY30_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPW64954.1"
                     /translation="RKRGHRTLNGLGMLFYQGVRAFECWTGGKAPVSVMRNALLEALR
                     TKGKP"
     gene            149..1309
                     /locus_tag="PLY30_02245"
     CDS             149..1309
                     /locus_tag="PLY30_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPW64955.1"
                     /translation="MIEMFYPVIIFVFGAIAGSFLNVCIHRMPRELSIVWPGSFCPKC
                     KIPIPWYENIPLLSYLILRGKCFRCRRPISFRYFFVELLNAVSWLLIWRSCGLSAAFA
                     IAAVFVSMMIVVTLTDLETGFIPHGVTWTGMIFGVCVSVVNVRLVPQGLWYHKLLASV
                     LGLVTGAATIFAVAELGKLLFGKRKVRLAPGTVITIKDNRLLIDQPGAPLASRAGGVS
                     GFFQRLADTLTRGQYLDPSCSSIAWDDIFNRSSDRIVFRAATLRFAGGNFKDVEVEIS
                     EKSFRVAGKEHALADAGVIEGRAEAMVLSREAMGFGDLCLMAMIGTFLGPWLTVLVFM
                     FAPFIALPYALFCRFAKKQETIPYGPFLAVMAVIFYFAGPEILDLFSTLYGV"
     gene            1313..2431
                     /gene="aroC"
                     /locus_tag="PLY30_02250"
     CDS             1313..2431
                     /gene="aroC"
                     /locus_tag="PLY30_02250"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPW64956.1"
                     /translation="MLNYITTGESHGKYLATILEGLPSGLAFDLDFVNAELRRRQGGF
                     GRGGRQKIETDCVEIMGGVIRGKTTGAPLGLILKNRDNTVDALPDLERPRPGHADLAG
                     ALKYHQSIRAILERSSARETSMRVAAGAACKLFLREFGIEIASHVVRIGPASLPERSL
                     TVKEIRALTKDSPVNCVCPKTARAMIRVIEKARKQSDTVGGKYEVRAAGLPAGLGSCV
                     HHARKLDGRLAGMLMSKQSVKAVEIGEGDRLGSLYGSGAHDEIFYSKSRGYYHRTNRA
                     GGIEGGMTNGEDVVVRVTMKPIATLARPLASVNMRTKRTEKADFERSDFCAVPAGSVI
                     GEALVAYVLADAFLEKFGGDSMREIRRNYDGYRRQIRG"
     gene            2446..>2650
                     /locus_tag="PLY30_02255"
     CDS             2446..>2650
                     /locus_tag="PLY30_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPW64957.1"
                     /translation="MNIYLTGMMGSGKSVTGKALAALTGYQFTDIDSEIEKQEGRPIV
                     EIFATDGESYFRDVETAVLKKISA"
BASE COUNT          487 a          711 c          887 g          565 t
ORIGIN      
        1 cccggaaacg cggtcaccgg accctgaacg gtctcgggat gcttttttat cagggcgttc
       61 gggcgttcga atgctggacc gggggaaagg ctccggtgag tgtgatgcgt aacgcgctcc
      121 tcgaggcgct caggacaaaa ggcaagccat gattgaaatg ttctaccccg ttatcatctt
      181 cgtgttcggc gcgatcgcgg ggagttttct gaatgtttgc attcaccgga tgccgcggga
      241 gctttcgatc gtttggccgg ggtcgttctg tccgaaatgc aagatcccga tcccgtggta
      301 cgagaatatt ccgctcctga gctatctcat tctccgcggc aagtgcttcc gctgcaggcg
      361 tcccatttcg ttccggtatt ttttcgtgga acttctgaac gccgtgagct ggctcctgat
      421 ctggcggagc tgcggtcttt cggcggcgtt cgcgatcgcc gcggtctttg tgtcgatgat
      481 gatcgtggtg acgctgacgg acctggaaac gggattcatc cctcatggcg tcacgtggac
      541 ggggatgatc ttcggggtct gcgtgagcgt tgtgaacgtg cgcctggtgc ctcagggctt
      601 gtggtatcat aagttgctcg cgagcgtgct cggtcttgtg acgggcgccg cgaccatttt
      661 tgccgttgcg gaactgggga aactgctctt tggaaaacgg aaagtccgtc tggccccggg
      721 gaccgtcatt actatcaagg acaaccgttt gctgatcgac cagcctgggg cccctctcgc
      781 gtcgcgcgcc ggcggagttt ccggtttttt tcagcgcctg gcggacactc tgacgcgcgg
      841 gcaatatctg gatccgtcct gcagctcgat cgcgtgggac gatatcttta accggtcttc
      901 ggaccggatc gtttttcgcg ccgcgacgtt gaggttcgcg ggcgggaatt tcaaggacgt
      961 agaggtcgag atctccgaaa aaagcttccg tgtcgccggc aaggaacacg cgcttgcgga
     1021 cgcgggcgtg attgagggga gagccgaggc gatggtcctt tcgcgcgagg cgatggggtt
     1081 cggcgacctg tgcctgatgg cgatgatcgg gacgtttctc gggccttggc tgacggtcct
     1141 tgttttcatg tttgcgcctt ttatcgcatt gccgtacgcg cttttttgcc ggttcgcgaa
     1201 aaaacaggaa acgatcccgt atggtccttt cctggccgtg atggcggtca ttttctattt
     1261 cgcggggccg gagattctgg accttttttc aacactatac ggagtctgac acatgctgaa
     1321 ttacatcacg accggagaat cacatggaaa gtaccttgcg acgatcctcg agggattgcc
     1381 gagcggtctt gcgttcgacc ttgatttcgt gaacgccgaa ctgcgccggc gccagggagg
     1441 cttcgggcgc ggcgggcggc aaaagatcga gaccgactgt gtcgagatca tggggggcgt
     1501 gatccggggc aagacgacgg gggcgccgct cgggctgatt ctcaagaaca gggacaatac
     1561 ggtggacgcg ttgccggacc tcgaacggcc gaggccggga cacgcggacc tcgccggggc
     1621 gctcaagtat caccagagca tccgcgcgat cctcgaacgc tcgagcgcgc gcgagacctc
     1681 gatgcgtgtc gcggccggcg ccgcctgcaa attgtttctc cgtgaattcg ggatcgagat
     1741 cgcgtcccac gtggttcgga tcggcccggc ctcgctcccg gagcggtcgc tgaccgtcaa
     1801 agagatccgg gcgctcacga aagattcgcc cgtcaactgt gtttgtccga aaacggcccg
     1861 ggccatgatc cgggtgatcg agaaggcccg gaaacaatcg gatacggtcg gcggaaaata
     1921 cgaggtccgc gcggcgggac ttccggccgg gctgggttcc tgtgtccatc acgcgagaaa
     1981 actcgatgga cgactcgcgg gaatgctgat gagtaaacaa tccgtgaagg ccgtggagat
     2041 cggcgagggg gaccgtctcg ggtccctcta cggttccggg gcccacgatg agatctttta
     2101 ttcaaaatcc cgcggctatt atcaccggac gaaccgcgcg ggtggtatcg aaggcggcat
     2161 gacgaacggg gaggatgtgg tggtccgggt gacgatgaaa ccgatcgcga ccctcgctcg
     2221 cccgctcgcc tcggttaaca tgcgcacaaa gcggaccgaa aaggcggatt tcgaacgcag
     2281 tgatttttgc gcggttccgg cgggcagcgt gatcggcgag gcgctcgtgg cgtatgtgct
     2341 cgcggacgcc ttcctcgaaa aattcggcgg agattccatg cgggagattc ggcggaatta
     2401 cgacgggtac cggaggcaaa tccgcggctg agaagcggga gagcgatgaa catctatctt
     2461 acggggatga tggggtcggg aaagagcgtg accgggaagg ctctggcggc cctgaccggg
     2521 tatcagttta cggacatcga ctccgagatc gagaaacagg aggggcggcc gatcgttgag
     2581 atcttcgcga cggacggtga gtcgtatttc cgggatgtgg aaacggcggt cctgaaaaaa
     2641 atctcggccg
//
LOCUS       DAOOBX010000114         2642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_42993_length_2642_cov_3.597217, whole genome shotgun sequence.
ACCESSION   DAOOBX010000114 DAOOBX010000000
VERSION     DAOOBX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42993_length_2642_cov_3.597217"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /gene="nuoL"
                     /locus_tag="PLY30_02260"
     CDS             <1..1654
                     /gene="nuoL"
                     /locus_tag="PLY30_02260"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPW64958.1"
                     /translation="IQIYASGYMAGDPRYSRFFAYVSLFMAAMLTLVISNNFILFFMA
                     WEIMGLCSYLLIGFYFERESAAKACFKAFLTTRVGDVGFLIGMLTLFTALGTLNFSEI
                     NGALAHGSARAAVPASLLVMSALFMFCGTVGKSAQFPLHVWLPDAMEGPTPVSALIHA
                     ATMVAAGVFLVARAFVIFMAVPETMPVVTAIGCFTAFMAAFIALTQTDIKRILAYSTI
                     SQLGYMVTALGMQGLGAGTFHLITHAFFKALLFLGAGSVIHGTHVQDIRQMGGLFRKM
                     PHTAITFIIASLALAGIPPLSGFWSKDEILLTAFASGNIVVFWVLTLSAFMTAFYMFR
                     LVFMTFFGKARDPHVHAHESPAVMTLPLWCLAIGSVVLGFPGSPFMHHWFQRFLAHAV
                     PHPEYHPSAFVMSCSVIAAVSGIALAGIIYLKNVHWAEAVAKAFRPLYQLSLNKFYVD
                     EIYSRFVIRPFLALGRVLFHFDATVIDGAVNGAGTGSMWLGAVKNWFDKNIVDGLVNF
                     VGAFTCFLNSVVKRLQTGLVQNYLLIAFLGFLVFIIRELKLI"
     gene            1716..>2642
                     /locus_tag="PLY30_02265"
     CDS             1716..>2642
                     /locus_tag="PLY30_02265"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPW64959.1"
                     /translation="MTSHLLSWITFTPLLGAFLLLCIPKRAHNSIRWIALATTAITAA
                     LVGLAFSLFRASEAGFQLVERAVWIPQFGVQYLLGMDGISFPMVVLTALISLMACLGS
                     LTHIRVKGGREKEYYFLFLLLETGMMGTFLALDLFLFYIFWEVVLVPMYFLIGIWGGP
                     RKEYAAIKFFLYTLAGSLFMLIGIVAVYFASSPHTFDMTLLTTMKYGLLFQKVVFLAF
                     FLGFAIKVPVFPFHTWLPDAHVEAPTPISVILAGVLLKMGTYGFLRFSYPLFPDAARW
                     FQPAMGVLAVVGIIYGGFIALAQTDFKKMVAYS"
BASE COUNT          402 a          854 c          726 g          660 t
ORIGIN      
        1 gattcagatt tacgcctcgg gctacatggc gggcgacccg cggtacagcc gcttcttcgc
       61 ctacgtttcc ctttttatgg ccgcaatgct cacgctcgtc atctcgaaca acttcatcct
      121 cttcttcatg gcgtgggaga tcatggggct gtgttcgtac ctcctgatcg gtttctattt
      181 tgaaagggaa tccgcagcga aggcctgttt caaggcattt ctcacgaccc gcgtcggtga
      241 cgtcggcttt ctgatcggca tgctgaccct cttcaccgcg ctcggcaccc tgaatttttc
      301 ggaaatcaat ggtgcgctcg cgcacggaag cgcgcgcgcc gccgttccgg cctcgctgct
      361 tgtgatgtcc gccctgttca tgttctgcgg gacggtcggg aaatccgcgc agttcccgct
      421 gcatgtctgg cttcccgacg cgatggaagg cccgacgccc gtcagtgcgc ttatccatgc
      481 cgccacgatg gtcgcggccg gcgtgttcct tgtcgcccgc gcgttcgtca tcttcatggc
      541 ggttccggag acgatgcctg tcgtgacggc gatcggctgc ttcacggctt ttatggccgc
      601 cttcatcgcc ctcacgcaga ccgatatcaa gcggattctc gcgtattcca cgatctccca
      661 gctcggctac atggtcacgg ctctcggcat gcaggggctc ggcgcgggaa cgttccatct
      721 gatcacgcac gcgttcttca aggccctgct cttcctcgga gcggggagcg tgatccacgg
      781 cacccacgtc caggacatcc gccagatggg cggacttttc agaaaaatgc cgcacacggc
      841 gattacgttc atcatcgcga gccttgcgct ggccggcatc cccccgcttt cgggcttctg
      901 gtcgaaagac gaaatcctgt tgacggcatt cgcctccggc aacattgtcg ttttctgggt
      961 cctgacgctt tcggctttca tgacggcctt ctatatgttc cgtcttgttt ttatgacctt
     1021 cttcgggaag gcgcgcgacc cgcacgtgca tgcgcacgaa tcgcccgccg tcatgacgct
     1081 tccgctctgg tgtctcgcga tcggctccgt cgtgctcggc tttcccggct cgcctttcat
     1141 gcaccactgg tttcagcggt tcctggccca cgcggtacct catccggaat atcatccgtc
     1201 ggcgttcgtg atgagctgtt ccgtgatcgc ggcggtctcc ggcatcgcgc tcgcggggat
     1261 catctacctg aagaacgtcc attgggccga ggcggtcgcg aaggccttcc ggccccttta
     1321 tcagctttcg ctcaacaagt tctatgtcga cgaaatctac tcgcggttcg tgatccgccc
     1381 cttcctcgcc ttgggccggg tccttttcca cttcgacgcg accgtgatcg acggcgcggt
     1441 gaacggcgcg ggcacgggct cgatgtggct cggcgcggtc aagaactggt tcgacaagaa
     1501 catcgtggac ggcctcgtga acttcgtggg cgcgttcacc tgtttcctga attcggtcgt
     1561 caaacgtctt cagacgggac tcgttcagaa ctacctgctg atcgcgttcc tgggttttct
     1621 tgttttcatc atccgggaac tgaaattgat ctaacggatt aagattcttc gtcctgcggc
     1681 cgcggaatga cgcgccgcac caaaaaggag tttttatgac atcgcattta ctgagctgga
     1741 tcaccttcac gccgctcctc ggggcattcc ttctgctttg cataccgaag cgggcccaca
     1801 actcgatccg ctggatcgcg ctcgcgacga ccgcgataac cgcggcgctc gtcggcctcg
     1861 cgttttccct tttccgcgcc tccgaggcgg ggtttcagct ggtcgaacgc gcggtgtgga
     1921 ttccccagtt cggcgtccag tacctcctcg gcatggacgg tatcagcttc ccgatggtcg
     1981 tcctgacggc ccttatctcg ctcatggcat gcctcgggtc gctcacccac atccgcgtca
     2041 agggtggccg tgaaaaggag tactacttcc tgttcctcct cctcgagacc ggcatgatgg
     2101 gcacctttct cgcgctcgat cttttcctgt tctacatctt ctgggaggtc gtcctcgtac
     2161 cgatgtactt cctgatcggg atctggggcg gaccgcggaa agaatatgcc gcgatcaagt
     2221 ttttcctcta cacactggcc ggcagtcttt tcatgctgat cgggatcgtc gcggtctact
     2281 tcgcttcgtc gccgcacacc tttgacatga ccctgctcac aacgatgaaa tacggcctcc
     2341 tcttccagaa ggtcgtgttc ctcgcgttct tcctcggttt tgcgatcaag gttccggtct
     2401 tcccgttcca cacatggctt cccgacgcgc acgtcgaggc cccgacgccg atctcggtca
     2461 ttctggcggg tgtgctcctc aagatgggca cctacgggtt cctgcgcttc agttacccgc
     2521 tttttccgga cgcggcccgc tggtttcagc cggccatggg cgtcctcgcg gtcgtgggca
     2581 tcatctacgg cggcttcatc gcgctcgcgc aaacggattt caagaaaatg gtcgcctatt
     2641 cc
//
LOCUS       DAOOBX010000115         2617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_43513_length_2617_cov_3.627635, whole genome shotgun sequence.
ACCESSION   DAOOBX010000115 DAOOBX010000000
VERSION     DAOOBX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43513_length_2617_cov_3.627635"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /gene="eno"
                     /locus_tag="PLY30_02270"
     CDS             complement(<1..508)
                     /gene="eno"
                     /locus_tag="PLY30_02270"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768223.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPW64960.1"
                     /translation="MAKTTITSIKAREILDSRGNPTVEVDVLLKDGTLGRAAVPSGAS
                     TGEHEAVELRDGDKSRYLGKGVLKAVKNANTTIAKALKGKDAAEQKKIDSLMIDLDGT
                     PNKARLGANAILGVSLACAQATAASKKVPLYAMIGGKKANLLPVPMMNIINGGVHADN
                     NVDLQEFMI"
     gene            complement(635..1213)
                     /gene="purN"
                     /locus_tag="PLY30_02275"
     CDS             complement(635..1213)
                     /gene="purN"
                     /locus_tag="PLY30_02275"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPW64961.1"
                     /translation="MKRIAFFVSGSGTNMANLVARIKAGEIRAEAALVVSNKPGVKAL
                     ERAKECGVPSEVISHEDFATREAFDAALAECVDKYRIDLICLCGFMRVLTPGFVRKYH
                     GRLINIHPALLPAFPGAHGIRDAWDARVRETGVTVHFVDDGVDTGPIILQRKVPVLPT
                     DTLETLEARIHAAEYEVYPEALRLVIEGKLQV"
     gene            complement(1235..1921)
                     /gene="rsmI"
                     /locus_tag="PLY30_02280"
     CDS             complement(1235..1921)
                     /gene="rsmI"
                     /locus_tag="PLY30_02280"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPW64962.1"
                     /translation="MLSIVATPIGNLGDITLRALEALKAADYIACEDTRETVKLLAHY
                     QVPEKRFLGYYEHSPEIRVHEILALVRQGKRVAVVTDGGMPSISDPGFTLIREAIREK
                     APFEVLPGASAVTTALVASGLAVDEFSFLGFLPNKSAARKKRLARFAEREETLIFFES
                     PFRLPSALRDMFEVFGDREAVVARELTKKFEELLRGRLSELVRDVEKRPRKGEMVVLV
                     SGQGRKKLLS"
     gene            complement(1926..>2617)
                     /locus_tag="PLY30_02285"
     CDS             complement(1926..>2617)
                     /locus_tag="PLY30_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPW64963.1"
                     /translation="IRRGLPSLHVKWGPQTAVLAGDYFHDRAVVTIFRYGNDRIIPLF
                     LKTAGEICEGEMQELGEKNNINLTEEIHFEILRKKTASLLACALQTGALVWGMDEARA
                     EALGRYGNYFGMAFQITDDCLDFTGRENELGKTLGSDLGEGVYTLPVIRCLASADRDK
                     VVRILHARNHGKRFSMLKTLLQKNGALEYALEKAKGFVSRAKAELEGFPESPFKTSLL
                     GLADYVIRRER"
BASE COUNT          498 a          831 c          744 g          544 t
ORIGIN      
        1 taatcatgaa ctcctggagg tcgacgttgt tatccgcgtg aacgccgccg ttgatgatgt
       61 tcatcatcgg taccggcaac aggttcgctt tctttccgcc gatcatcgcg tagagcggga
      121 ccttcttcga agccgcggtg gcctgcgcgc acgcgagcga aacaccgagg atggcgttcg
      181 cgccgagccg agccttgttc ggcgtaccgt cgagatcgat cataaggctg tcgatcttct
      241 tctgttcggc cgcgtccttg cctttgaggg ctttcgcgat cgtcgtattc gcgtttttga
      301 cggctttcag cacgcccttg ccgagatagc gggacttgtc gccatcgcga agctcgaccg
      361 cctcgtgttc gccggtggac gcgccgctcg ggaccgccgc gcggccaagg gtgccatcct
      421 tcaaaaggac gtcgacttca acggtcgggt tgccgcggga atcgagaatc tcgcgtgcct
      481 tgatgctggt gatcgtggtc tttgccatgg gattactcct tgattgtgtc cggaacgagc
      541 tggaaaacgc gcgtgtcagg agggtttaat gtgaaaaaaa cggtcaactt cgcgggcgat
      601 tatagcaaat cctcccccgc tgtccagcgt ccggtcagac ctggagcttt ccttcgatca
      661 cgagccggag cgcttcggga tagacctcat attcagctgc gtgtatccgg gcttcgagcg
      721 tttcaagagt atccgtcgga agcaccggca ctttccgctg gaggataatg ggaccggtat
      781 ccacgccgtc atccacaaaa tggaccgtga cgccggtctc cctgaccctg gcatcccagg
      841 cgtccctgat gccgtgcgcg ccggggaacg ccgggagcag cgccgggtga atgttgatca
      901 aacggccgtg atattttctg acgaaaccgg gcgtcaggac ccgcatgaac ccgcagagac
      961 agatgaggtc gatcctgtat ttatcgacgc attcggccag cgccgcgtca aaagcctcac
     1021 gcgtcgcgaa atcctcgtgc gaaatcactt cagaaggaac accgcactct ttcgcccgct
     1081 cgagcgcctt cacgccgggt ttgttcgaga ccacgagcgc ggcttcggcg cggatctccc
     1141 ccgccttgat cctcgcgacc agattggcca tgttcgtgcc gctgcctgaa acaaaaaaag
     1201 cgatgcgttt cattttcacc cccggtacgg cgtactagga aagtagtttt ttccggccct
     1261 gccccgacac caacactacc atctcaccct ttcggggccg cttttccaca tcccgaacga
     1321 gctccgacaa ccggcctctc aggagttctt cgaatttctt cgtgagctcg cgggccacaa
     1381 ccgcctcccg gtcaccgaag acttcgaaca tatcccggag cgccgacggc aagcgaaacg
     1441 gcgattcaaa aaagatcagc gtctcctccc gctccgcgaa ccgcgcgagc cgcttcttcc
     1501 gtgccgcgct tttgttcggg agaaacccga ggaacgagaa ttcgtcgacc gcaaggccgc
     1561 tcgcgacaag cgccgtcgtc accgcggacg cccccggcaa aacttcaaaa ggtgcctttt
     1621 cccggatcgc ctcacggatg agcgtgaacc ccggatccga gatcgacggc atgcctccgt
     1681 ccgtcacgac tgcgacccgc tttccctgcc tcaccagcgc aaggatctca tggacgcgga
     1741 tctcgggcga atgttcgtaa tagcccagga atcgtttttc gggaacctgg taatgcgcca
     1801 ggagtttgac ggtctcccgc gtgtcctcgc aagcgatgta atccgcggcc ttcagggctt
     1861 ccagggcccg taaagtgata tcccccaggt ttccgatcgg cgtcgcgaca atgctcagca
     1921 tggaatcacc gctcccgccg gatcacgtaa tccgcaagac cgagaaggct cgttttgaaa
     1981 ggcgattcgg ggaatccctc aagctccgcc ttggcccgcg agacgaatcc cttcgccttt
     2041 tccagcgcgt actcgagcgc gccgttcttt tggaggaggg ttttcagcat tgagaaccgt
     2101 ttcccgtggt tccgcgcgtg gagaatcctg acgaccttgt cacggtcggc cgacgcgagg
     2161 cagcggatca cggggagcgt gtagacccct tccccgagat ccgagccgag cgtctttccg
     2221 agctcgtttt cgcggccggt gaaatcaagg caatcgtccg tgatctggaa cgccatcccg
     2281 aaataattcc cgtagcggcc gagggcctcc gcgcgggcct cgtccatccc ccacacaagc
     2341 gctccggtct ggagcgcaca ggcaagaagt gacgccgttt tcttccggag gatctcgaaa
     2401 tgaatctcct cggtcagatt gatattgttc ttctcgccga gctcctgcat ctcgccttcg
     2461 cagatctcac ccgcggtttt gaggaagaga ggaatgatcc ggtcgttccc gtaccgaaag
     2521 atcgtgacga ccgcccggtc gtggaaataa tcgccggcca gcacggcggt ctggggcccc
     2581 cattttacgt gaagcgacgg caacccgcgg cggatcg
//
LOCUS       DAOOBX010000116         2592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_44068_length_2592_cov_3.130075, whole genome shotgun sequence.
ACCESSION   DAOOBX010000116 DAOOBX010000000
VERSION     DAOOBX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44068_length_2592_cov_3.130075"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLY30_02290"
     CDS             <1..418
                     /locus_tag="PLY30_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW64964.1"
                     /translation="LKRIKGNFSEIEEVLFNLLDNAADAIAMKEEAFRLGTLAKPEGW
                     EKGSIRFEAENAELNGKPHVRILVRDNGIGMDAETQGKLFVPFFTLKATAVKGTGLGL
                     YIIRKMIDAHQGHIQLESVPGEGTVFSVFLPALAGG"
     gene            420..773
                     /locus_tag="PLY30_02295"
     CDS             420..773
                     /locus_tag="PLY30_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW64965.1"
                     /translation="MARIVFVDDEADMLGTLRDAFTELGHEVHTAEDGREGLRLILDK
                     EPDLAFVDMRLGGMKGMEILREVKAQKPGIKTIMLTGYNDSQIDADAMKHGALMCLHK
                     PVGLKELADIIEKYA"
     gene            1159..1356
                     /locus_tag="PLY30_02300"
     CDS             1159..1356
                     /locus_tag="PLY30_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HPW64966.1"
                     /translation="MDCKVSANRKRCNCTYEPCPRKGICCECLAHHRERNELPACYFT
                     ASAERTYDRSVDAFLRMRGAV"
     gene            1382..1813
                     /locus_tag="PLY30_02305"
     CDS             1382..1813
                     /locus_tag="PLY30_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64967.1"
                     /translation="MRTKFFKIAGLAVLVLCVASLGVVAYAEDEGGTTHKGWSKGRKT
                     GWQGESTPPGFAKREGRKAGRNMKETTDEAGEIAKEAREKADLEAGKTQSGADKEMKR
                     AQKKADKETRKARKKAEKEARKKQKEAGKAAKATRTAAGAL"
     gene            1892..>2592
                     /locus_tag="PLY30_02310"
     CDS             1892..>2592
                     /locus_tag="PLY30_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW64968.1"
                     /translation="MVKQQKKKAIIIGTSSGIGRALALRLSGEGYVVGLTGRDPEKLK
                     TLQSELAGESFTRQIDLRDPREAIHSLNNLIMQLHGLDLLVVNAGVLFRNEELDWRKE
                     EETAKVNVLGFMVMADTAVNYFLKSGAGTLVGISSVAGHRGSGRSPAYNASKAFMINY
                     MEGLRQKLFGTRIKVVDIRPGFVDTAMIRGNKGLFGVVTAERAVADIYRAIRKGKRIA
                     YVPGWWGLFMWFAKR"
BASE COUNT          611 a          679 c          874 g          428 t
ORIGIN      
        1 cctgaagcgg atcaagggga acttcagcga gatcgaggag gtgctcttca acctgctcga
       61 caacgcggcg gatgcgatcg cgatgaagga ggaggccttc cggctcggga ccctcgcgaa
      121 accggagggt tgggagaagg gaagtatccg gttcgaggcg gagaacgcgg agctgaacgg
      181 gaagccgcat gtgcggattc tcgtgcgtga caacggtatc ggcatggacg cggagacgca
      241 ggggaagctg tttgtgccgt tttttacgct gaaggcgacg gcggtgaagg gaacggggct
      301 cgggctttac attatccgca aaatgatcga cgcgcatcag ggacacattc agctggaatc
      361 ggtgcccggc gaggggacgg tcttctctgt cttcctgccg gcgctcgcgg gaggatgaca
      421 tggcaaggat cgtttttgtg gacgacgaag cggatatgct cggcacgttg cgggacgcct
      481 tcacggagct cgggcacgaa gtccacacgg cggaggacgg gcgggaaggg ctccggctga
      541 ttctcgataa ggaacccgac ctcgcctttg tggacatgcg gctcggggga atgaagggca
      601 tggagattct ccgcgaggtg aaggcgcaaa agcccggcat caagacgatt atgctcacgg
      661 ggtataacga cagccagatc gacgccgacg cgatgaaaca cggggcgctc atgtgcctcc
      721 acaaacccgt cggactcaaa gagcttgccg atatcattga aaaatacgcc tgaccggagg
      781 aggtcggagt tatgacggaa atcccgaagc cctgcggtct gaaggacttg tatgaacgga
      841 tcgaaagggt gatctagcag aacgggttca aggtcccggt cggggtttgt caggaccggc
      901 gatggaacgg agagtgtctg gcccggtggc ggaccggccc ccggaagcgc aggtccggtg
      961 cccgcaaaag acgatcgacg cagggacaga gaggttggat tgcaaatcct cgccgagctc
     1021 aaggcccgcc tccccaggat ttgacgacgc tcgggcgtgg aaacggaaaa gtttcgtgat
     1081 ataatccacg gtattcgaaa gatcccgcag cgtattaccg ggattccgtg tctgttcttc
     1141 aacccgagag gttcgtggat ggattgcaag gtctcagcga acaggaaacg atgcaactgc
     1201 acttatgaac cgtgcccccg gaaaggcatt tgctgtgaat gcctggccca ccaccgggag
     1261 cggaatgaac ttcccgcgtg ctattttacc gcgtcggccg agaggaccta tgaccgttcg
     1321 gttgacgcgt tccttagaat gcgcggggcc gtctgacaac cccaacaagg agaaactcac
     1381 catgcgtacg aaattcttca agattgccgg attggccgtt ctggtcctgt gcgtcgcgtc
     1441 gctcggcgtt gtcgcctatg ccgaagacga aggcgggaca acccacaagg gatggagcaa
     1501 gggtagaaag accggatggc agggggaaag cacgcctccc ggattcgcga agcgggaagg
     1561 ccggaaagcc ggaaggaata tgaaggaaac gaccgacgaa gccggggaga tcgcgaagga
     1621 ggccagggaa aaagccgatc ttgaggccgg gaagacgcag tccggggccg acaaggaaat
     1681 gaagagggcg cagaaaaagg ccgacaagga aaccaggaaa gcccggaaga aagccgagaa
     1741 agaggccagg aaaaaacaga aagaagccgg gaaggccgcg aaagcgacaa ggacggccgc
     1801 gggcgctctc tgatccggac gtgaagaccg aaaaggcgtt gcccgccagg acgggggcaa
     1861 cgcctttttt ataaaaggac ctgacagtca tatggtgaaa caacagaaga aaaaggcgat
     1921 cattatcggg accagcagcg ggatcggccg cgcgctggcg ctcagacttt cgggggaagg
     1981 gtacgtggtc ggtctcaccg gccgcgaccc tgagaaactc aagaccctcc agtctgaact
     2041 cgcgggcgag agcttcacgc gccagatcga tctccgcgac ccccgcgagg cgatccacag
     2101 tctcaacaat ctcatcatgc agctgcatgg cctggacctc cttgtcgtta acgcgggcgt
     2161 cctcttccgc aacgaagagc tcgactggag gaaggaggag gagaccgcga aggtcaacgt
     2221 cctgggcttc atggtcatgg cggacacggc cgtgaactat ttcctcaaaa gtggcgcggg
     2281 caccctcgtc gggatctcgt cggtcgcggg ccatcgcggg agcgggcgct cgccggccta
     2341 caacgcctcg aaggcgttca tgattaacta catggagggg cttcgccaga aacttttcgg
     2401 gacgcggatc aaggtcgtcg acatccggcc cgggttcgtg gacaccgcga tgatccgcgg
     2461 caacaagggg cttttcggtg tcgtgacggc ggaacgtgcc gtggccgata tttaccgggc
     2521 gatccggaaa ggaaaacgta tcgcgtacgt tcccgggtgg tgggggcttt tcatgtggtt
     2581 cgccaaacgg at
//
LOCUS       DAOOBX010000117         2587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_44198_length_2587_cov_3.017378, whole genome shotgun sequence.
ACCESSION   DAOOBX010000117 DAOOBX010000000
VERSION     DAOOBX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44198_length_2587_cov_3.017378"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /gene="aroB"
                     /locus_tag="PLY30_02315"
     CDS             <1..796
                     /gene="aroB"
                     /locus_tag="PLY30_02315"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPW64969.1"
                     /translation="AVGGGVVSDLSGFAASVYLRGIRYVNVATTLLAQVDSSIGGKTG
                     INLPEGKNLMGAFHPPALVISDVGVLDSLPAKELCASLAEVVKYGVIRDARLFRFLER
                     NAGRILEKNAAALERIVTDSACIKAGVVSRDEYETKGERMILNYGHTFGHGIENAAKY
                     RKIVHGEAIGIGMAMAAHLAAEIGICSPEFVRRQLALLKRLGLPVAVDNLKIRSATVL
                     KAMMRDKKKAGGRLRFVLPERIGKVTIKTGISAGELRNILRLFGAR"
     gene            1036..2199
                     /gene="dprA"
                     /locus_tag="PLY30_02320"
     CDS             1036..2199
                     /gene="dprA"
                     /locus_tag="PLY30_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPW64970.1"
                     /translation="MNSMSDRDLASMLVLNRLGIRERRRAVELLREGQYPGEVLEVLN
                     EEGTKEPFDPLKELDECRREGVALCTVLDAEYPAALKEIPDPPLLFYMKGMLETGDAN
                     AVAVVGTRHPSFYGRTQARRFSGELAAKGITIISGLARGIDQAAHEAALGVPYGRTIA
                     VLGCGIDVVYPKENEKLSGKITERGALISEYPLGTPPLAENFPRRNRIISGLSHGVLV
                     IEAHSRSGSLITAHEALDQGREVFALPGPVDQLTSRGTHQLIREGAALIESPEEIFEA
                     LADRWWSVAPPESFVSATAEESATGVPIQPVPVNKEEEASVVVNEETLPGEEKDEESA
                     ILLEALREESGMTVDEILEMTGLDAARVATLLFMLELGGRIRKTPDGRYFMTK"
     gene            2343..>2587
                     /locus_tag="PLY30_02325"
     CDS             2343..>2587
                     /locus_tag="PLY30_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64971.1"
                     /translation="MEGLVPAGKGKRFAAGIIDLVVVPIVLGVVIALALFMASDAVRN
                     IVLIFINVAWLIFRDAVWAPGRAMIKTRVISLLGGKVT"
BASE COUNT          538 a          671 c          830 g          548 t
ORIGIN      
        1 cgcggtcggc ggaggtgtcg tcagcgatct ttccggtttc gcggcctccg tgtatcttcg
       61 cgggatccgt tatgtgaatg tcgcgacaac cttgcttgcc caggtcgaca gttcgattgg
      121 cggcaagacg gggatcaacc ttcccgaagg gaagaacctg atgggcgcgt tccatccccc
      181 ggcgcttgtg atctccgatg tcggtgtcct ggattcgctt cccgcgaaag aactttgcgc
      241 gtcgctcgcg gaagttgtga aatacggcgt gatccgcgac gcgcggctgt tccggttcct
      301 cgaaaggaac gcggggagga ttcttgaaaa gaacgcggcg gcgcttgagc ggattgtaac
      361 cgactccgcg tgtatcaagg ccggtgtcgt gagccgggac gaatacgaga cgaagggtga
      421 acgaatgatc ctgaactacg ggcatacatt cggtcacggg atcgagaacg cggcaaaata
      481 cagaaaaatc gttcacggcg aagcgattgg gatcggcatg gcgatggcgg cgcaccttgc
      541 cgccgagatc gggatatgct cgccggaatt tgtgagacgc cagctcgcgc ttttaaagag
      601 gctcggcctg cccgttgccg ttgacaacct gaagatccgg tccgcgacgg tcctgaaggc
      661 catgatgcgg gacaaaaaga aagcgggggg ccgtctccgc ttcgttctcc ccgaacggat
      721 cggcaaggtt acgatcaaga ccggtatctc tgccggcgaa ctccgcaata ttctccgcct
      781 tttcggcgcc cgataaccgc tttttcagag agaggaaggc ggcgctcaaa catttgctat
      841 aaatcaatat attattgatt tatagcaaaa atcatgttag gcttgccgca tgaggagcag
      901 gcgcaccccc ataccacaca ggcggatctt ccggaccatt gttttctttt gcggacgaaa
      961 gatgatggct gatacggtcc gttgttccgc agacccgggg aacctccggg aaagacgacc
     1021 ggcaggatat catctatgaa ctccatgtcc gaccgggacc ttgcctccat gcttgtttta
     1081 aatcgccttg ggatccggga aaggcggcgg gcggtggagc ttttacgcga aggacaatat
     1141 cccggcgagg tcctcgaagt cctgaatgag gagggaacga aagagccgtt cgatccgtta
     1201 aaggagctcg atgagtgccg gagggaaggg gtcgcgttat gcacagtcct cgacgcggag
     1261 tacccggccg cgttgaagga gatccccgat cctccgcttc ttttttacat gaaagggatg
     1321 cttgagaccg gtgatgccaa tgccgtggcg gtggtcggga cgcggcatcc gagtttttat
     1381 ggcagaacgc aagccaggcg tttttcggga gagcttgccg cgaaagggat cacgatcatc
     1441 tccggtctcg cccgcggcat tgaccaggcg gcgcacgagg cggccctcgg ggttccctac
     1501 ggcaggacga tcgcggtgct tggatgcggt atcgatgttg tctatcccaa agagaatgaa
     1561 aaactgtccg ggaagatcac ggagcggggg gcgctgatca gcgaatatcc gctcgggacc
     1621 ccgccgcttg ctgaaaattt cccgaggcgg aaccggatca tctccgggct cagtcacggc
     1681 gtgcttgtga tcgaagccca ttcgcggagc ggttcgctga ttaccgccca cgaggcgctc
     1741 gaccagggca gggaggtgtt cgcgctcccg gggccggtcg atcagctgac gtcgcgcggc
     1801 acccatcaac tgatcaggga gggagcggcc ttgatcgaaa gcccggagga gatctttgaa
     1861 gcccttgctg accgctggtg gtccgttgct cctcccgaat ctttcgtgag cgccacggcc
     1921 gaggaatccg ctaccggtgt accgatacaa cccgtgccgg taaacaagga ggaggaagcg
     1981 tcggtggtcg tcaatgaaga gacactcccg ggagaagaga aggatgagga gtcggcgatc
     2041 ctgctcgaag ccctgcgtga ggagagcggc atgacggttg atgagattct tgaaatgacg
     2101 ggactggatg cggcaagggt cgcgacgctg ctgtttatgc tggagcttgg ggggcggatc
     2161 cggaaaactc cggacggcag gtattttatg actaaatgaa atccggaggc tgccgatgct
     2221 taggttatcg gggcgttctt gtcagaaaaa gtttttgaca gcctcgttcc gaagggctag
     2281 attccagtgg ggtgtgacgt ctttttaggg cttcgggctg tataaacgaa aggagattcg
     2341 caatggaagg actggttcca gcaggcaaag gtaaaaggtt cgcggccggg attatcgatc
     2401 ttgttgtcgt tccgatcgtt ctcggtgttg ttatcgcgtt ggccctcttc atggcgtcag
     2461 atgcggttcg caatatcgtg ctcatcttca tcaacgtcgc ctggctgatt tttcgcgacg
     2521 ccgtttgggc ccccggtcgc gcgatgatca agacgcgcgt catcagcttg ctcggaggaa
     2581 aggtgac
//
LOCUS       DAOOBX010000118         2584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_44261_length_2584_cov_3.816133, whole genome shotgun sequence.
ACCESSION   DAOOBX010000118 DAOOBX010000000
VERSION     DAOOBX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44261_length_2584_cov_3.816133"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(559..1986)
                     /locus_tag="PLY30_02330"
     CDS             complement(559..1986)
                     /locus_tag="PLY30_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW64972.1"
                     /translation="MRASRFAPVLIAFFTVLPFLFPVTQARAEDVGQEVKAVERELRS
                     EKYSAAVAEKSGKLEEEKKYRTGVTYEDILKDPDNLPLNFRFAQQQIANNELLGSAAT
                     LERILLVNPNLADVRLLYAVVLYRLDNLNEAQKELETLKGMPLPKNIEEQVREYRKRI
                     ERRKRRTHFGLRETVGWGYDTNRNAAPSSKFQLVNDVAQGLEGTNRKRDDTHFTNITT
                     VDFTHDLGMRAGHEIFGSFTYFLQEQTMVDSLDLSTYQGEIGGRYKTKWVNISPSFIA
                     SNILLSRETYLRTLGLNLQFDRQLTRKLNGFMNFRYEYQNYSGVSENTTGAQRKGPEY
                     DYLWGVTYAPVATMRITPSIGYMNKNAKSGFNGYDRFNMNLIHTWALWRGSFLINAAN
                     LYFDRYNEPETDVASRFRHDKIFRYRVTVGTPLSAFLSRILPRQMEDIVFTLSYEYYR
                     SLSNITNYAYTNNKIEGLFTKRWEF"
     gene            2148..>2584
                     /locus_tag="PLY30_02335"
     CDS             2148..>2584
                     /locus_tag="PLY30_02335"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW64973.1"
                     /translation="MEFQLDPFQQQAVEWIEKDHSLLISAPTGSGKTLIAEKAVEKAF
                     AQGKGVIYTAPIKALSNQKFRDFQQRFGAANVGILTGDVAINAGARLLIMTTEIYRNS
                     LFENSERIRSTGWVIFDEVHYLDDHERGTVWEEALLFTPPEIR"
BASE COUNT          578 a          710 c          724 g          572 t
ORIGIN      
        1 acaaactcgt caagcttgat gcttgaagac gcaccgatcg tgaaaacggt ctgatcgaca
       61 agaaggatct gcatccttga atccgggctt gtcgtgaccg tgtcgccccg gaagagcgga
      121 tctccgcctt tcagcacacg cgtttcaccg gccgaagtgg tcgctttcac ctccccggta
      181 atccccgccg cgacaccgat tttcgccgaa gtatccggca aggccgcgac caagctgccg
      241 caaatcaaaa accccgccac gcacaaacaa gccaccagac tccacgattt gtttttcatt
      301 ttcccctctc ctcagcttca aaactgaacc aacgttggat tagcaaagaa aacggaaccc
      361 gcttcgcgca tagtatagct gcgggggatt gtttgagata gatgaaaatt ccgcaggaca
      421 acgcttcctc ttcaagcaac cggaacaaga tctgaagagt tctcaatgtc aaccgtcaac
      481 ggcaacggga ggctgcggcg cttgagctaa ttgacggcgc gcgggagaca tcggggcgta
      541 accccgattc cgatctccct agaattccca gcgcttggtg aacaggcctt caatcttgtt
      601 attcgtgtag gcgtaattcg tgatgttgga gagcgagcgg taatactcgt aggaaagtgt
      661 gaacacaata tcctccatct gcctggggag gatcctggag agaaaagcgg aaagcggggt
      721 cccaaccgtc acgcggtagc ggaatatctt atcgtgacgg aaacggctcg cgacatccgt
      781 ttcgggttcg ttataacggt cgaaataaag gttcgcggca ttgatcagga acgaaccccg
      841 ccagagcgcc cacgtgtgga tcaggttcat attgaaccgg tcgtagccat tgaacccgga
      901 cttggcgttc ttgttcatat agccgatgct cggcgtgatc cgcatcgtcg cgaccggcgc
      961 gtacgtgaca ccccacaggt aatcatattc cgggcctttg cgctgcgccc cggtcgtgtt
     1021 ctccgagacg ccggaataat tctggtactc gtaacggaag ttcatgaaac cgttcagttt
     1081 tctcgtgagc tggcgatcga actgaaggtt cagccccagc gtccggaggt aggtttcgcg
     1141 ggaaagcagg atattgctcg cgataaagga cggcgaaata ttaacccact tggtcttgta
     1201 ccgcccgccg atctccccct ggtaggtgct gaggtcgagc gaatcgacca tagtctgttc
     1261 ctgcaggaaa tacgtaaaag acccgaaaat ctcgtggccg gcccgcatgc cgaggtcgtg
     1321 cgtgaagtcc acggtcgtaa tgttcgtgaa gtgcgtgtcg tcgcgcttgc ggttcgtgcc
     1381 ttcgagacct tgtgccacat cgttcacgag ctggaacttg gaggaaggcg ccgcgttgcg
     1441 gttcgtgtca tagccccagc ccacggtctc ccggagaccg aaatgcgtcc ggcgcttgcg
     1501 gcgttcgata cgtttcctgt attccctgac ctgttcctcg atgttcttcg gaagcggcat
     1561 gcccttcagg gtctcgagct ctttttgggc ctcattgagg ttatcgagac gatacagcac
     1621 gacggcataa aggaggcgga cgtccgcgag attcggattg acgaggagga tccgctcgag
     1681 ggtggcggcc gagccgagga gctcgttgtt ggcgatctgt tgctgagcga accggaagtt
     1741 cagcggaagg ttatcgggat ccttgaggat atcttcatag gtcacgccgg tccggtattt
     1801 tttctcctct tcgagctttc cggatttttc agcgacagcg gcggaatatt tttccgaacg
     1861 gagttcccgc tcgacagcct tgacctcctg cccgacatct tcggcacggg cctgcgtgac
     1921 ggggaaaaga aacgggagga cggtgaaaaa agcaatgagg accggagcga atctggaagc
     1981 gcgcatgaga gccctttctt taacgggttc ggatttgtga cagtgtacta taggaacacc
     2041 ctaaaaatca aaaagagatt accgcttcct tttttagcgt ccgctatccg gaaaaccgct
     2101 ccaaataacg gctttgtcgc ttttgaaatc aagatatact agtgcccatg gaattccagc
     2161 ttgatccttt ccagcagcaa gccgttgagt ggattgaaaa agaccactct ctcctcattt
     2221 cggccccgac cggttccggc aagaccctca tcgcggaaaa ggccgtcgaa aaggccttcg
     2281 ctcaggggaa gggcgttatc tacacggccc cgatcaaagc cctgagtaat cagaagttcc
     2341 gcgatttcca gcagaggttc ggcgcggcca atgtcggcat cctgaccggc gacgtcgcga
     2401 tcaacgcggg cgcccggctg ctcatcatga cgaccgagat ttaccggaac tcccttttcg
     2461 agaacagtga gcgcatccgg agtacgggtt gggtcatttt cgacgaggtc cattacctgg
     2521 atgaccacga gcgcgggacg gtctgggagg aggcccttct tttcacgccg cctgagatca
     2581 ggat
//
LOCUS       DAOOBX010000119         2563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_44712_length_2563_cov_4.297049, whole genome shotgun sequence.
ACCESSION   DAOOBX010000119 DAOOBX010000000
VERSION     DAOOBX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44712_length_2563_cov_4.297049"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLY30_02340"
     CDS             <1..982
                     /locus_tag="PLY30_02340"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPW64974.1"
                     /translation="LLSSFACTVPGVLATRTIEDRNERLATVMVAPLMSCSARLPIYT
                     LLAAAFIPPGKIFGILSMQGVTLLVMYLLGIGAGLGMAAIFRKTFLRGNRMPFVFELP
                     PYRMPHMRNIISAMWDRSSEFLVRAGSVIFLLSIVLWFCVSYPKNPAAEAGFVQDRHE
                     AAATLEGDALGERFEQIEESRKDLQIRSSYAGMLGRALEPVIRPLGFNWEIGVGIVSS
                     FAARELLISSLAIVHHVKGEGEGMTAGLIDALRSARDPDTGKPLYTPLVAIGLMVFYV
                     FACQCFSTYAVLKRELGSWGWTFFTIGYMTLLAWGSAFLVYQGGRLLGFQ"
     gene            1096..1230
                     /locus_tag="PLY30_02345"
     CDS             1096..1230
                     /locus_tag="PLY30_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64975.1"
                     /translation="MDKSELVALGIVAVAALVLLIRFFKKGGGGGCGADCLKRKGHEQ
                     "
     gene            1395..2225
                     /locus_tag="PLY30_02350"
     CDS             1395..2225
                     /locus_tag="PLY30_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPW64976.1"
                     /translation="MFRPEPTKLTVVDATPVDPGHIEWVNTCSITGSRKQWRTNGRRA
                     KRKFLREHSFETQANIGVFNGFDFGITQGFAYLLDKENNYDETGGAVDADGESLDETD
                     GPHRGHGRTDLILNGRWRFYQSCDETLRVAYVPSITIPVGRRSNLDHLGPSQGYVSMG
                     HTLAVTRDMNRWTMSGNAGYEVPIAHKARRENYAGTLTLAAGAGYHVFDWLQPQVEVI
                     YEHDFEQSGKGSKLVSMVFGAIIPVNDHLRLDLGVVQGIAGSNADQTTSGVFKLVLLT
                     "
     gene            complement(2269..>2563)
                     /locus_tag="PLY30_02355"
     CDS             complement(2269..>2563)
                     /locus_tag="PLY30_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPW64977.1"
                     /translation="AVSLPLHRDGFSKKKSFLYGWLSGIVEPAAAVIGALIVVAVQPL
                     LPFALAFAAGAMIFVVVEEVIPESQQNGNGDLATTGTILGFALMMLLDVAFGK"
BASE COUNT          517 a          670 c          821 g          555 t
ORIGIN      
        1 gctcctgagt tccttcgcgt gtacggtgcc gggggtgctg gcgacgcgta cgatcgagga
       61 ccggaacgag cggcttgcga ccgtgatggt ggcgccgctt atgagctgca gcgcgcggct
      121 tccgatctac acgcttcttg cggccgcatt cattcctccg gggaaaatct tcgggattct
      181 ctccatgcag ggcgtgacac ttcttgtgat gtaccttctc ggaatcgggg ccggccttgg
      241 gatggcggcg atcttccgaa agaccttcct gaggggaaac cggatgcctt ttgtgttcga
      301 actgccgccg taccggatgc cccacatgcg gaacattatt tcggccatgt gggaccgctc
      361 gagcgaattc ctggtccggg cgggcagtgt tatctttctg ctttcgatcg tgctctggtt
      421 ctgcgtgagc tatccgaaga atcccgcggc cgaagccggg ttcgtgcagg accgccacga
      481 agcggcagcg acgctcgaag gcgacgcgct cggggaacga ttcgagcaga tcgaagaatc
      541 ccgcaaggac cttcaaatcc gttcaagcta cgcggggatg ctgggccggg ccctcgaacc
      601 cgtgatccgc ccgctcggtt ttaactggga gatcggggtc gggatcgtct cttcgttcgc
      661 ggcccgtgaa cttctgatca gttcgctcgc gatcgtccat catgtgaaag gcgaaggcga
      721 ggggatgacg gccgggctga tcgacgcatt gaggtctgcg agggacccgg acaccggaaa
      781 gccgctttac acgccgctcg tggcgatcgg gctcatggtt ttttatgtgt tcgcgtgcca
      841 atgtttttcg acctacgcgg ttctgaaacg cgaactcggt tcttggggct ggacgttctt
      901 tacgatcggc tacatgacac tgctagcctg gggcagcgcg tttcttgtat atcagggggg
      961 aaggttgtta gggtttcagt aaaaacagcg ccacgggcca agcagaaccg tttccccatg
     1021 gttcgtaacc ctctacgccc gcgcgggatg cgataaatca cattgcgtag gtgttgtgag
     1081 gtccagagga gttttatgga caaatcagaa cttgttgcgc tcgggatcgt cgccgtagcg
     1141 gcgctcgttc tgctgatccg cttttttaaa aaaggcggcg ggggcggctg cggcgcggat
     1201 tgtttaaaga gaaaggggca tgaacaatga aggttttttt tctcgcggtt atttttttat
     1261 caacggttcc tggcccctcc tgggcggcgg actttgatcg gaaggattat gaggtacaca
     1321 cgtccccgaa cggacacatt tacgggtccg gaaaacattc ttctgccaaa gcttcggtcg
     1381 cgcaggaggc agaagtgttc cgaccggaac cgacgaagct cacggtggtg gacgcgacac
     1441 cggtcgatcc cgggcatatc gaatgggtga acacctgctc gatcacggga tccaggaaac
     1501 agtggcggac gaacgggcgt cgcgcgaagc ggaaattttt gagggagcac agttttgaga
     1561 cgcaggccaa tatcggcgtc tttaacggtt ttgatttcgg gatcacgcag ggattcgcgt
     1621 atttgctgga taaagagaac aactacgatg aaacgggcgg ggcggtggac gcggacgggg
     1681 agtcgctgga tgagaccgat ggtccgcacc ggggacacgg ccggacggat ctgatactga
     1741 acggccgttg gcgtttttat cagtcctgcg atgaaacctt gcgcgttgct tacgttcctt
     1801 cgattactat cccggttggc aggcggagca acctggacca tcttggaccg tcgcagggtt
     1861 atgtcagcat ggggcataca ctggctgtca ccagggatat gaaccgctgg accatgagcg
     1921 ggaacgcggg atatgaagtt ccgatcgccc ataaggcaag gcgggaaaat tacgcgggga
     1981 cattaacgct tgccgcgggg gcgggatatc acgtgttcga ttggctccag ccccaggtgg
     2041 aagtgatcta tgaacatgat tttgaacagt cggggaaagg ttcaaagctt gtcagcatgg
     2101 tctttggcgc gattatcccg gtgaacgatc accttcgcct tgatctcggt gttgtgcagg
     2161 gtattgcagg cagcaacgcg gaccagacca cgagcggagt cttcaaactg gttcttctga
     2221 cttaacaccg ctcacccccg attccggaat cggtccgtaa ccggcgggct atttcccgaa
     2281 cgcgacgtcg aggagcatca tgagggcaaa gccgaggatg gtgccggtcg tcgcgagatc
     2341 gccgttgccg ttctgctggg actcggggat cacctcttcc acgacgacga aaatcatggc
     2401 ccccgcggcg aaggcgagag cgaacggcag aagcggttgg acggcgacca cgatgagcgc
     2461 gccgataacg gccgcggccg gttccacgat cccggacagc catccgtaaa gaaagctttt
     2521 cttttttgaa aaaccgtctc ggtgaagagg gagggaaacg gcg
//
LOCUS       DAOOBX010000120         2546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_45098_length_2546_cov_3.026495, whole genome shotgun sequence.
ACCESSION   DAOOBX010000120 DAOOBX010000000
VERSION     DAOOBX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45098_length_2546_cov_3.026495"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1182)
                     /locus_tag="PLY30_02360"
     CDS             complement(121..1182)
                     /locus_tag="PLY30_02360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPW64978.1"
                     /translation="MKILVVMKNWLGDLLFQMPALEVIKSRYPGAEIFCVAPERCRAI
                     LEAHPAVAGFLPFDEKSTHRSWFSRAGFVFRLRKLGPWDEGYLFHRSRSRAVMLCLAG
                     VKKRTGYGKGRSLFLTTAVLEPDGKLHQLDYFMTLMKGAGYETPIEPKSRFYFTPGDD
                     REAREVLRQNGLTDGARYVCFHLGANWKPKRWPVEHFAVLASMVHAKWKIPIVVTGSA
                     GDRTLFMDFLKSLRRLGAPGADELRVIDLVGKTPLRVSACIYKHATCLVTGDSGPMHI
                     ASGVGARVAAIFGPTDPDLTGPRGVGESIVLQYVPPGYSIPFFGKDLPGDGWLSGITP
                     DEVFEAVQQLGAFPDGSGS"
     gene            complement(1301..>2546)
                     /gene="waaF"
                     /locus_tag="PLY30_02365"
     CDS             complement(1301..>2546)
                     /gene="waaF"
                     /locus_tag="PLY30_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HPW64979.1"
                     /translation="ATWLGGVTDIIDHGVNGFLFPPKSVTAMAEAMYEMLTDRAKARE
                     YATALRKKIEEKFALGQMVRKTVEVYQEVRKKKKILVIKLGAMGDLVLVVPSLRMLKE
                     RYPDASITLLVDKRLSRIMADCPYLDDMILVDRKKFPNPLYLLKTARRVRRENFDVSV
                     DLHNNKYTYLLAYLGGAIRRYGFARGFWGSLLNRPDKTFRIAAEPVRHQFRVISKAGV
                     QDYDDTLELWPGKEQVEKAERVLLSLDLKADTKVIGFVMGSSPKWPTKRWPAAHYRKL
                     AERIFDRYDARILLFGSPGEAELANEFRDFDPDRVVNLIGKTGIEDLPAFFQKLHFVV
                     SGDTALLHVAAAMHVPIAAIFGPTDPKRHVPPAKKIAVFMRNLPCQPCYSGVCKEKET
                     MGCLAHISVDEVFDTAKRFLEA"
BASE COUNT          540 a          799 c          728 g          479 t
ORIGIN      
        1 gccttcggcc cgacaaccac cgtgatccgc gcagccggga accttgcgcg aagcgccatg
       61 atcaccggtg tcgtcagcac gacatcgccg atattgctga gcgtcacaac aaggatgctt
      121 tcaggatcct gagccatcgg ggaaagcacc taattgttgg accgcttcaa agacctcatc
      181 gggcgtgatc ccggaaagcc agccatcccc ggggaggtcc ttgccgaaga acgggatcga
      241 ataacccggc gggacatact gcaggacgat cgattcgccg acaccgcgcg gtccggtaag
      301 atcggggtcc gtgggaccga agatcgcggc cacgcgcgcc ccgacaccgg aagcgatatg
      361 catcggcccg gaatcccccg tcacaagaca tgtcgcgtgt ttgtaaatac aggcggagac
      421 ccggagcggc gtctttccga ccagatcgat cacgcgcaat tcgtcagccc cgggcgctcc
      481 caggcggcga agtgatttca gaaaatccat gaaaagggtt cggtcccccg cggaacccgt
      541 caccacaatc gggatcttcc acttcgcgtg aaccatggag gcaagtaccg caaaatgctc
      601 caccggccag cgttttggct tccagttcgc tccaagatgg aaacagacat accgcgcgcc
      661 gtccgtgagc ccgttctgcc ggaggacctc gcgggcctcc cggtcgtctc cgggtgtgaa
      721 ataaaagcgg gacttcggct cgatgggcgt ttcataaccg gcgcccttca tgagggtcat
      781 aaagtagtcc agctgatgga gcttcccgtc cggctccagg accgccgtcg tgaggaaaag
      841 gctccgccct tttccgtagc cggtgcgttt tttcaccccc gcgagacaaa gcatgacagc
      901 ccgcgaacgg gaacggtgga aaagataacc ttcatcccag gggccgagtt tccggagccg
      961 gaaaacgaaa ccagcccggg agaaccagga gcgatgcgtg gatttttcgt cgaatggaag
     1021 gaatccggcg accgccggat gcgcttcgag aatcgcgcgg caacgttccg gcgcgacgca
     1081 gaaaatctca gccccgggat agcggctctt gatcacctca agcgccggca tctggaaaag
     1141 cagatcgccg agccagttct tcatcacgac aagtattttc acactgtcag agatgcttca
     1201 agacatctct cctccgtttt aaaaccgtta aaatctttct caaacaaacc cgcccgcaag
     1261 atcacggccc cgccaaaggc gtgacctgac ccgaacgggg tcatgcctca agaaaccttt
     1321 tggcggtgtc aaaaacctca tccaccgaaa tgtgggccag gcatcccatc gtctcctttt
     1381 ccttgcagac cccgctgtag cagggctggc atgggaggtt ccgcatgaac acggcgatct
     1441 ttttcgccgg cgggacatga cgcttcgggt ccgtcgggcc gaagatcgcg gcgatcggga
     1501 catgcatcgc ggccgcgaca tggagaaggg ccgtgtcccc ggagactacg aaatgcagct
     1561 tttggaagaa cgcgggcagg tcctcgatcc cggtcttccc gatcaggttc acgacccggt
     1621 ccgggtcgaa gtcgcggaat tcattcgcaa gctccgcttc gccgggcgaa ccgaaaagaa
     1681 gaatccgcgc gtcgtagcgg tcaaagatcc gttccgcgag cttccggtaa tgcgccgccg
     1741 gccaccgctt ggtcggccac ttgggactcg atcccatcac gaaaccgatg accttcgtat
     1801 cggccttgag atcgagcgaa agaaggacac gctccgcctt ttcgacctgc tccttgccgg
     1861 gccagagctc cagcgtatca tcatagtcct ggactccggc cttggagatc acccggaact
     1921 ggtggcggac cggttctgcc gcgatccgga acgtcttgtc gggacggttc aggagtgagc
     1981 cccaaaaccc gcgcgcgaac ccgtaccgcc ggatcgcgcc gccgagatac gcaaggagat
     2041 acgtgtactt gttattgtga agatcgaccg aaacgtcgaa gttttcccgc cgcacgcgac
     2101 gggctgtctt cagaagatag agagggttcg gaaacttctt ccggtcgacc aggatcatgt
     2161 cgtcgaggta ggggcaatcc gccatgatcc gcgaaagccg tttatcgacg agaagcgtga
     2221 tcgatgcgtc ggggtaccgt tccttcagca tccggaggct cggcaccaca aggacgaggt
     2281 cccccatcgc tcccagtttg attacgagaa tctttttctt cttccggacc tcctggtaga
     2341 cctcgacggt cttccggacc atctgcccga gcgcgaactt ctcctcgatc ttcttccgca
     2401 gggcagtcgc gtattccctt gcctttgcgc gatccgtcag catctcatac atggcctccg
     2461 ccatcgcggt cacgcttttc ggcggaaaga ggaaaccgtt cacgccgtgg tcgatgatgt
     2521 ccgtgacacc cccgagccac gtcgcg
//
LOCUS       DAOOBX010000121         2545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_45116_length_2545_cov_3.600402, whole genome shotgun sequence.
ACCESSION   DAOOBX010000121 DAOOBX010000000
VERSION     DAOOBX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45116_length_2545_cov_3.600402"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2149)
                     /gene="carB"
                     /locus_tag="PLY30_02370"
     CDS             complement(<1..2149)
                     /gene="carB"
                     /locus_tag="PLY30_02370"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPW64980.1"
                     /translation="MPKRNDIKKILIIGSGPIIIGQACEFDYSGTQACKALRKEGYEV
                     VLVNSNPATIMTDPEMADHTYIEPLTVESLEKIIAKERPDALLPNLGGQTGLNLSSAL
                     HKSGVLEKYGVKIIGVKADAIERGEDREVFKATMAQIGIPVPRSATANSVEECESIAQ
                     QLGYPVVVRPAYTMGGTGGGIAYNVEDLRVIALRGLTISLARQVLIEEAVIGWEELEL
                     EVVRDAKDQKITVCFIENVDAMGVHTGDSFCVAPMLTVAPEIQKKLQEYSYKIVDAIG
                     VVGGTNVQFAHNKKDGRVVVIEINPRTSRSSALASKATGFPIAMVSTRLAAGLTMDEI
                     PYWRDGSLEKYTPGGKHVVVKFARWAFEKFPRAQDVLGTQMKAVGEVMSIGKNFKEAF
                     QKAIRSLEIGRYGLGDVKDFRQLSDDQLRERLARPSSERIFLMYEALRKGLSVEELHA
                     MTHIGKWFITEMRELVMFEEAMLKATWDKFADRDLIKAKEWGFSDRYLAGLFKISEAA
                     VRERRIRLGKVARYEAVPVSGVQNAAYYYSTYHPGREDAPVSTNKKILILGGGPNRIG
                     QGIEFDYTCVHAVFALRAAGYESIMINCNPETVSTDYDTANKLYFEPLTVEDVLAIYE
                     KEKPEGVIVQFGGQTPLNIAQQLKDAGVRILGTSPESIAFAEDRELFRQKMIELGIPQ
                     PESGIARSIEESIAIAREIGYPIMVRPSFVLGGR"
     gene            complement(2219..>2545)
                     /locus_tag="PLY30_02375"
     CDS             complement(2219..>2545)
                     /locus_tag="PLY30_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HPW64981.1"
                     /translation="HRMLGQALGGKTSRIKFGYHGNNHSVMDLKSRRIGIAFRNHGFV
                     TDIDLLPKDEAELTHTNLNDKSLGGLARRKCPPFRAQYHPENASGPHDNDYLFSRFYK
                     LTERNA"
BASE COUNT          507 a          778 c          685 g          575 t
ORIGIN      
        1 cgcgcccgcc gagaacgaag gagggacgga ccatgatggg gtagccgatc tcacgcgcga
       61 tcgcgatgga ttcctcgatc gaccttgcga tgccgctttc cggctgagga atgccgagct
      121 cgatcatctt ctgacgaaag agctcgcggt cttcggcgaa cgcgatgctc tccggcgacg
      181 tcccgaggat cctgacgccg gcgtccttga gctgttgagc gatgttgagc ggtgtctgcc
      241 ctccgaactg gacgatgacg ccttccggtt tttccttctc atagatcgcc agcacgtcct
      301 cgaccgtaag cggttcaaaa tagagcttgt tggccgtgtc ataatcggtc gagaccgttt
      361 cggggttgca gttgatcatg atcgattcgt agcccgccgc gcggagcgcg aagaccgcgt
      421 gcacgcaggt ataatcgaac tcgattccct gcccgatgcg attcggcccg ccgccgagga
      481 tcagtatctt cttgttcgtt gagacgggag catcctcccg gcccgggtgg taggtggaat
      541 aatagtacgc ggcattctgg acgccgctca caggaaccgc ttcgtaccgc gcaaccttgc
      601 caagccggat gcgccgttcc cgcaccgccg cttccgagat tttaaaaagt ccggcaagat
      661 aacggtccga gaatccccat tccttggcct tgatgaggtc ccgatccgcg aacttgtccc
      721 aggtcgcctt caacatcgct tcctcgaaca tcaccagctc ccgcatttcc gtgatgaacc
      781 acttcccgat gtgggtcatg gcgtgaagct cttcgacgct caggcccttg cggagggctt
      841 cgtacatcag aaagatccgt tcgctggaag gccgggccag tctttcgcga agctggtcgt
      901 ctgaaagctg cctgaagtcc ttcacatcgc cgagcccgta ccggccgatc tcgagggaac
      961 ggatggcctt ctggaaagcc tctttaaaat tcttcccgat cgacattact tcgccgaccg
     1021 ccttcatctg ggtcccgaga acatcctgcg ccctggggaa tttctcgaac gcccaccgcg
     1081 cgaatttaac gacaacgtgc tttccgccag gcgtgtattt ttcgagcgat ccgtcgcgcc
     1141 agtaaggaat ctcatccatc gtcagaccgg ccgcgagacg cgtcgacacc attgcgatcg
     1201 ggaatcccgt agccttcgac gccagcgccg aggaacgcga cgttctcggg ttaatctcga
     1261 ttacaaccac gcgcccatcc tttttattgt gggcgaactg aacattcgtt ccgccgacca
     1321 caccgatcgc gtccacaatc ttgtaggaat attcctgcag tttcttctgg atttcggggg
     1381 cgaccgtcag catcggcgcc acgcagaagc tgtcgcccgt gtggacaccc atcgcgtcca
     1441 cattctcgat aaagcagacc gtaatcttct gatctttcgc gtcgcggacc acttccagtt
     1501 caagctcttc ccatccgatc acagcctcct cgatcagaac ctgacgggca agactgattg
     1561 tcagcccacg aagagcgatc acacgcaaat cctcgacgtt ataggcgatc ccgccgccgg
     1621 taccgcccat cgtataggcc ggacggacca cgaccggata gccgagctgc tgcgcgatcg
     1681 attcacattc ctcgacggaa ttcgccgtcg cggaccgcgg cacgggaatc ccgatctgcg
     1741 ccatggtcgc cttgaagacc tcacgatcct ccccgcgctc gatcgcgtcc gctttaacac
     1801 cgatgatctt gacaccgtat ttttcgagaa caccgctttt gtggagcgcc gaggaaagat
     1861 tcaggcccgt ctgccctccg agattgggca acagcgcatc gggacgctct ttcgcgatga
     1921 tcttttcgag gctttcgacg gtcaacggtt cgatgtaggt atggtccgcc atttcgggat
     1981 cggtcatgat ggtcgcaggg ttcgagttca cgagcacgac ctcataccct tccttgcgca
     2041 aggctttgca ggcctgcgtt ccggaataat cgaattcaca cgcctgaccg atgatgatcg
     2101 gcccggaccc gataatcagg attttcttga tgtcattacg ttttggcatg atcttccgct
     2161 aagctccgag agataagggc ccggagctgt tcccggctcc aggatctgct tcctgagctt
     2221 aagcattcct ttctgtaagt ttgtaaaacc gtgagaagag atagtcatta tcgtgcggac
     2281 cggaggcatt ctccggatga tattgcgcgc gaaacggggg gcatttcctg cgagcaagac
     2341 ccccgagcga tttgtcgttc agattggtat gggtcagctc ggcctcatcc ttcggaagca
     2401 aatcgatatc cgtaacaaag ccgtggttcc ggaatgcgat cccgatcctg cggctcttca
     2461 gatccatgac ggaatgatta ttcccgtgat aaccaaattt gatcctggag gtctttccgc
     2521 caagcgcctg gccgagcatc cggtg
//
LOCUS       DAOOBX010000122         2544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_45148_length_2544_cov_2.418642, whole genome shotgun sequence.
ACCESSION   DAOOBX010000122 DAOOBX010000000
VERSION     DAOOBX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45148_length_2544_cov_2.418642"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLY30_02380"
     CDS             <1..714
                     /locus_tag="PLY30_02380"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPW64982.1"
                     /translation="DVIVITAGLARKPGMSRDDLLFRNAEIVGGIVTECAKYSPDAVI
                     VMVTNPLDVMTWLAQKKSGFPAERVIGMAGELDSARYSYFIAEKLGCKPSDVRAMVLG
                     GHGDQMVPVPEHTKVNGKPVTQLMDAATIEAINRRTRDGGAEIVKLLKTGSAYYAPSS
                     SAVKMVRAILRDTGETIPSCVYLKGPYGLNGVYCGVPAKLGKGGLKEVVEIQLTDGER
                     AELHRSAQDVLRNFEKLKL"
     gene            793..>2544
                     /gene="acnA"
                     /locus_tag="PLY30_02385"
     CDS             793..>2544
                     /gene="acnA"
                     /locus_tag="PLY30_02385"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009802237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HPW64983.1"
                     /translation="MNLLNSFGSKVTLEIAKKSYTIYRIRALQECGVANVLKLPYAIR
                     VLLENLLRNEDGKNVTRRDIENLANWSPRKKSTQEIAFTPARVLLQDFTGVPAVVDLA
                     AMREAMVRMGGDPKKINPLQPVDLVIDHSVQTDFYGAPDAFRKNMEKEFERNRERYQF
                     LKWGQKAFNNFRVVPPATGIIHQVNLEYLSPLVFKRKEGGKTYLYPDTLVGTDSHTTM
                     INGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFRMTGKMKEGITITDVVLTVTQML
                     RKKGVVGKIVEYFGTGAESLSLADRAVISNMAPEYGATAGYFPVDDETLAFMKLTGRP
                     DDLICLAEEYLKEQSLFRENPNEAIEYSDVLELDLRTVEPGLAGPKRPQDRVALHDVK
                     TSFEKSLPDLLKGKGSPDKKISIEIEGQKCELENGAVVIASITSCTNTSSPSAMVTAG
                     LLAEKAALKGLVPKPWVKTSLAPGSQVVVDYLRDSGLLYYLEKMRFHLAGYGCMTCIG
                     NSGPLPDPVSWAIKEGDLVAASVLSGNRNFEGRVHAQVKANYLATPALVVAYALAGTV
                     LIDMEKDPVGKDKEGKPI"
BASE COUNT          591 a          675 c          749 g          529 t
ORIGIN      
        1 gatgtgatcg tgatcacggc cggcctcgcg cgcaaacccg gcatgagccg tgacgacctc
       61 cttttcagga acgccgagat tgttggcggt attgtcacgg aatgcgcgaa gtattccccg
      121 gatgccgtca tcgtgatggt cacgaacccg ctcgatgtga tgacctggct cgcgcagaaa
      181 aagtcgggat tccccgcgga gcgagtgatc ggcatggcag gcgagcttga ttcggcgcgt
      241 tactcgtatt tcatcgccga aaagctaggt tgcaaacctt ccgatgtccg ggcaatggtg
      301 ctcggcggcc acggtgacca gatggtcccg gtgccggagc acacaaaggt gaacggtaaa
      361 cccgttacgc aactcatgga tgcggcgacg atcgaagcga tcaaccggcg gacgcgcgac
      421 ggcggcgcgg aaatcgtgaa acttctcaag accggcagcg cctactacgc cccctcatcc
      481 tcagccgtca aaatggttcg cgcaattctc cgggataccg gcgaaacgat cccgtcctgt
      541 gtttatctga aaggcccgta tggcttaaac ggcgtttatt gcggtgtgcc cgcgaaactc
      601 ggaaaaggcg gtttaaagga agtcgtcgaa atccagctca cggacggcga gcgcgcggaa
      661 cttcacaggt cggcgcagga tgttctgcgg aattttgaaa agctgaaact ttaaaaaggg
      721 acacgttccc gggaccgggg attcgattca agctattgtt ttacgagacc aggtcccttt
      781 aggattttaa aaatgaacct tctcaacagc ttcggaagca aggtcacgct cgagatcgcg
      841 aagaagagct acacgatcta ccgcatccgc gctcttcagg agtgcggagt tgccaatgtc
      901 ctgaagcttc cctacgcgat acgcgtcctc cttgagaatc ttctccgcaa cgaggacggc
      961 aagaatgtca cgcgccgcga catcgagaac cttgctaact ggtctcccag gaagaaaagc
     1021 acccaggaaa ttgcgttcac gcccgcgagg gtgcttctcc aggattttac cggggtcccg
     1081 gccgttgtcg atcttgccgc gatgcgtgag gcgatggtca ggatgggagg agatccgaaa
     1141 aaaatcaatc cgcttcagcc cgtcgatctt gtgatcgatc actcggtgca gaccgatttt
     1201 tacggtgccc cggatgcttt ccgtaaaaac atggaaaagg aatttgaaag aaaccgggag
     1261 cgctaccagt ttcttaaatg gggacagaag gcgttcaata atttccgagt ggtcccgccg
     1321 gctacaggca ttatccatca ggtaaacctc gagtaccttt cgcctctcgt tttcaaacgc
     1381 aaggaggggg ggaaaaccta cctttatccg gatacgctgg tcgggaccga ttcgcatacg
     1441 accatgatta acgggctcgg cgttctcgga tggggcgtcg gcgggatcga ggcggaggcc
     1501 gcgatgctcg gacagcccgt ttcgatgctg atcccggaag tggtcggatt cagaatgacg
     1561 ggtaagatga aagaaggaat cacgatcacg gacgtggtcc ttacggtcac gcagatgctc
     1621 cgcaagaaag gcgttgtcgg caagattgtg gaatatttcg gtacgggtgc ggagtcgctt
     1681 tccctggccg accgcgccgt gatctccaat atggctccgg aatacggcgc gaccgcgggc
     1741 tattttcccg ttgacgatga gacgctggcg ttcatgaaat tgacgggacg gccggacgac
     1801 ctgatctgtc ttgcggaaga atatttgaaa gagcaaagcc tgttccgcga gaatccaaac
     1861 gaagcgatcg aatattccga cgtgctggag ctggatctga gaaccgttga accgggcctc
     1921 gcgggaccca aacgtcccca ggatcgtgtt gccctccatg acgtcaaaac gtcttttgaa
     1981 aagtcccttc ccgatcttct gaaaggcaaa ggaagcccgg ataaaaaaat ctcgatcgag
     2041 attgaagggc aaaaatgcga gcttgaaaat ggcgcggtcg tgattgcttc catcacgagc
     2101 tgcaccaata cttcgtcgcc gtccgcgatg gtgacagcgg ggcttctcgc cgaaaaagcc
     2161 gctcttaagg ggctggtccc caaaccctgg gtcaagacaa gccttgctcc gggatcccaa
     2221 gttgtcgtgg actacctccg cgacagcggc cttttgtatt acctcgaaaa aatgcggttt
     2281 cacctcgcgg gctacggctg catgacctgc atcgggaatt cggggccttt gccggatccg
     2341 gtttcctggg cgatcaaaga gggcgatctt gttgcggctt cggtgctttc cgggaatagg
     2401 aatttcgagg ggcgggtgca cgcccaggtc aaggcgaatt atctggctac tccggcgctt
     2461 gtagtcgcct acgcattggc gggaacggtc ctgatcgaca tggaaaagga cccggtcgga
     2521 aaagacaagg aaggaaaacc tatt
//
LOCUS       DAOOBX010000123         2540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_45227_length_2540_cov_2.597586, whole genome shotgun sequence.
ACCESSION   DAOOBX010000123 DAOOBX010000000
VERSION     DAOOBX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45227_length_2540_cov_2.597586"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /gene="amrB"
                     /locus_tag="PLY30_02390"
     CDS             complement(<1..763)
                     /gene="amrB"
                     /locus_tag="PLY30_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPW64984.1"
                     /translation="MEKRSPASGNNGVSHHFPKTVTDPVFVFAATLLSLAFTFSLPGD
                     KKESANTPKVRKAAVAGAFYPANKNQLAAYVDELLRRANPPPIAGSVKAILVPHAGYV
                     YSGPVAAHAYKTIARQDIQTVILMCNSHRQGFNGIAVYGNGSFETPLGPIEIDSDKTA
                     KLLAANPGIMDRPDIHEGDHVLEVQLPFLQRTLKNFKIVPLLFGTDDPALSRTLADAL
                     AKIVDDRTLIVASSDMSHYPPYDAASEADRKTDEAI"
     gene            complement(732..1847)
                     /gene="amrS"
                     /locus_tag="PLY30_02395"
     CDS             complement(732..1847)
                     /gene="amrS"
                     /locus_tag="PLY30_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPW64985.1"
                     /translation="MIKRIIAILFLAAVLGSAAVYILRRESAVTPQGNPDSYFEGYYY
                     EPLEQKRVQCFLCPNHCNLAEGKFAVCKARKNIGGKLYTMNYGRIASLHVDPVEKKPF
                     FHFLPGSNVFSVGTTGCSMRCFFCQNWEISQAFPDDVQTRPATPRQLVDEAVKSGAPV
                     IAFTYNEPVVAFEYMIETFKLAKAKGLRTAVVSSGYIAEEPLRELLKYTDAYKVDLKA
                     FSEKFYRKLTGGHLEDILRTLKIIKESRVWLEIVTLLIPGENDSPEEISSLARWVREN
                     LGTDVPLHFSRFFPKYKLNNLPPTPPETLIAARSIAMQEGLRFVYTGNVANPEGETTY
                     CPGSGETAIARQGYFITRNNLTDGACPNGEKIPGVWK"
     gene            complement(1858..2472)
                     /locus_tag="PLY30_02400"
     CDS             complement(1858..2472)
                     /locus_tag="PLY30_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPW64986.1"
                     /translation="MDKSLLIQPLVAGLSVGAFCLTRCFPFMAPLVAAEDRPLHRNLF
                     IVLEFLTGRLAGYLCFGILAGWLGERFGSRWLKLGTDLSFILLSIVLVFYLLGLIREQ
                     GLFCRASTFFRTRSPALMGFLMGINICPPFLLSVLYVFAQKSPFYGMVYFALFFLASS
                     VYFLPVIFLGMLARAKELRAIARLSGFLAAFIFFFYGIYSLFRL"
BASE COUNT          568 a          679 c          786 g          507 t
ORIGIN      
        1 ggatcgcctc gtccgtcttc cggtcggctt cgctcgccgc gtcgtagggc ggatagtgcg
       61 acatgtcgga actggcgacg atcagggtcc tgtcgtccac gatctttgca agggcatcgg
      121 cgagcgtccg ggagagcgcg ggatcgtcgg tcccgaaaag gagagggacg atcttgaagt
      181 tcttgagcgt tctctgcagg aaagggagtt gaacctcaag cacatggtct ccctcgtgaa
      241 tgtccgggcg atccataatc cccgggttcg cggcaaggag cttggccgtt ttatcggaat
      301 cgatctcgat tggcccgagc ggcgtttcga aacttccgtt cccgtagacg gcgatgccgt
      361 tgaagccctg gcgatggctg ttacacatga ggatgacggt ctgaatgtcc tgcctcgcga
      421 tggtcttgta agcgtgcgcc gcgaccggac cggaatagac atagcctgcg tgcggtacga
      481 ggatcgcctt gacgcttccc gcgatcggag gcgggttcgc ccgacgcaac agctcgtcca
      541 cgtaggcggc gagctgattc ttgttggccg gataaaaggc tcccgcgacg gccgccttcc
      601 ggaccttcgg ggtgttcgcg gattctttct tgtcccccgg gagggagaac gtgaaggcga
      661 gagaaagcag tgtggccgca aaaacaaaaa cggggtcagt caccgtttta gggaaatggt
      721 gactgacccc gttatttcca gacgccgggg atcttttctc cattggggca ggctccgtcg
      781 gtgaggttgt tccgggtgat gaaatagccc tgtcgcgcga tcgccgtctc tcccgatccc
      841 gggcaatacg tcgtttcacc ttcgggattg gcaacgttcc ctgtgtaaac aaaacgcagg
      901 ccctcctgca tcgcgatcga tcgcgccgcg atcagggttt cgggaggcgt gggcggcagg
      961 ttgttcagtt tgtatttcgg gaaaaaccgg ctgaagtgga gggggacgtc cgtcccgagg
     1021 ttctcccgga cccagcgggc gagggagctg atctcttcgg gggagtcatt ctctccggga
     1081 ataagaagcg ttacgatctc aagccacact ctgctttcct tgatgatctt cagggtccgc
     1141 aggatatcct cgagatgacc gcccgtgagc ttccggtaga acttttcgct gaaggccttg
     1201 aggtccactt tgtaggcgtc cgtgtatttc agaagttccc gcaggggttc ttccgcgata
     1261 tacccgctcg agacgacggc ggtgcgcagg cctttcgctt ttgccagctt gaaagtctcg
     1321 atcatgtatt cgaaggcaac cacgggttca ttgtaggtga aggcgatgac cggagcgccg
     1381 cttttgacgg cctcatcgac aagctggcgg ggcgtcgcgg gccgggtctg aacgtcgtca
     1441 gggaaggctt gcgaaatctc ccagttctgg cagaagaaac agcgcatgct gcatccggtc
     1501 gtcccgacgg aaaacacgtt acttccgggc aggaaatgaa agaacggttt tttttcgacc
     1561 ggatcgacgt gcagggaggc gatccttccg taattcatcg tgtacagctt gccgccgata
     1621 ttcttgcgtg ccttgcagac ggcaaacttc ccttcggcca ggttacagtg gttcggacac
     1681 aggaagcact ggacgcgctt ctgttcgagc ggctcataat aatagccttc aaaataagaa
     1741 tccggattac cctgcggagt cacggcgctt tcgcggcgca ggatataaac cgccgcggaa
     1801 ccgagaacgg cggcaaggaa caggatggcg atgattcgtt tgatcataag gtcagtttca
     1861 cagtctgaaa agggaataga tcccgtaaaa aaagaaaata aacgccgcga ggaatccgct
     1921 caagcgggcg atcgcccgca gttccttcgc tctcgcgagc attccgagaa agatcacggg
     1981 tagaaaatac accgaacttg ccaggaagaa aagcgcgaaa tagaccatgc cgtagaacgg
     2041 gcttttttgc gcgaacacat agagcaccga gaggaggaaa ggcggacaga tattaatgcc
     2101 catcaggaac cccatcagcg ccgggctcct ggtccggaag aatgtcgaag ccctgcagaa
     2161 gagcccctgt tcccggatga ggcccagaag atagaagaca aggacgatcg agaggagaat
     2221 gaaggaaaga tcggtgccga gtttcagcca ccgggagccg aatctttcgc cgagccagcc
     2281 cgcgaggatc ccgaaacaaa gataacccgc aagacgtccc gtcaggaatt ccaggacgat
     2341 gaaaagattc cggtgcaggg ggcggtcctc cgccgcgacg agcggcgcca tgaaagggaa
     2401 gcagcgggtg agacaaaaag ctcccaccga aagacccgcg accagaggct ggatgagcag
     2461 ggatttgtcc atgcctgtat gctagaaagg gaacccggat tggggaagtg aaatcttgag
     2521 catcgtccca cgtcccgaac
//
LOCUS       DAOOBX010000124         2535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_45352_length_2535_cov_3.054032, whole genome shotgun sequence.
ACCESSION   DAOOBX010000124 DAOOBX010000000
VERSION     DAOOBX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45352_length_2535_cov_3.054032"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLY30_02405"
     CDS             complement(<1..809)
                     /locus_tag="PLY30_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW64987.1"
                     /translation="MKKRRILRLILLLAVLAALVAGGAVRVVLSRVAASDEFRIFAEQ
                     RAGEYLKAKVSIGNIRPYRFNQLALENVIIEFPPMGGGSQLVRLDRLIFCYHLSQLWN
                     RNLEAPAGVTFRNPSMIVENGDFPYQYFETPARAEMPALNFKGGEIRYHIPTIGREIV
                     LRDIDGKVTPDAELKVRIDIRASASGFLEGDVFVRGVVDPVRRTHDLWLELGGMDFSR
                     DIPLPFRGLTGKIHWTGKNLVFNDVATVVHGWSAKLSGKFSSEDGKPQLSFR"
     gene            complement(816..>2535)
                     /locus_tag="PLY30_02410"
     CDS             complement(816..>2535)
                     /locus_tag="PLY30_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPW64988.1"
                     /translation="DKRMIVLDLALMVAGTKYRGQFEERIKAVMDEIRRADNVIIFID
                     ELHTLVGAGGAEGAIDASNILKPSLSRGEIQCIGATTLDEYRKYIEKDAALARRFQTV
                     IVDPPSVDDTIQILKGLRDKYEAHHRVKITDEALSEAAKLSDRYISGRFLPDKAIDII
                     DEAASRARLSVTTLPKDLKKMETEIESLRREKEASIKGQDFEKAAKLRDEERKAKEDL
                     QKMKKSWKESKSEVEIQVTGEDIAFVVSKWTGVPLARLEEKETARLLKMEEEIHKRII
                     GQDEAIRAIAHAVRRSRSGLRNPRRPIGTFIFLGPTGVGKTLLARALAEFMFGDEDAI
                     ITVDCSEYGEKFNVSRLVGAPPGYVGYEEGGQLTAKVRRRPYSVVLLDEIEKAHPDVF
                     NILLQLMEEGRLTDSFGRKVDFRNTIVIMTSNVGADLVRKQGDLGFTVKKDDLNYDNL
                     KTKLLDETRRVFRPEFLNRVDDIIVFHPLTRQDLEQIIDIELGEVKDRLKEKKMELLL
                     SDEVLKFLIDQGYDPVFGARPLKRTIQRHIENILAEEILGGKFREGDKIRAEVRGDRI
                     VFEKAT"
BASE COUNT          538 a          743 c          697 g          557 t
ORIGIN      
        1 cgaaaactca gttgcggctt accgtcctcg ctcgaaaatt tgcccgatag cttcgccgac
       61 cagccgtgga cgaccgtcgc gacatcatta aagacgagat tcttgccggt ccagtgaatt
      121 ttaccggtca ggccgcggaa cgggagcgga atatcacggg agaaatccat gcctccgagt
      181 tcaagccaca gatcatgtgt ccgccggacg gggtccacca ccccgcgcac aaacacatcc
      241 ccttcgagaa atccgctggc agacgcccgg atatcgatcc ggaccttcaa ttcggcgtcg
      301 ggcgtgacct tgccatcgat atcccggaga acgatctccc ttccgatggt gggaatatga
      361 tagcggatct ctccgccctt gaaattgagg gccggcatct cggcccgggc gggagtttca
      421 aaatactgat acgggaaatc cccattttca acaatcatcg acggattccg gaacgtcacc
      481 ccggccggag cctccaggtt gcggttccac aactggctga ggtgataaca gaaaatgaga
      541 cggtccagac ggaccagctg agagccgcct cccatcgggg gaaattcgat gataacattc
      601 tcaagcgcaa gttggttgaa acggtagggg cggatattgc cgatcgaaac cttagcctta
      661 aggtattctc cggcgcgttg ttcagcaaag atccggaact cgtcggatgc ggcaacccgt
      721 gaaagaacca cacgaacggc ccctcccgcc accagcgcag ccagaaccgc cagcagaagg
      781 atgagtctga ggattcggcg ttttttcaca aggcgtcagg tcgctttctc aaaaacgatc
      841 ctgtcaccgc ggacttccgc tcgtatcttg tcaccttccc ggaacttgcc gccaaggatt
      901 tcctcagcaa gaatgttttc gatatgccgc tggatcgtcc gcttgagcgg tcgggcacca
      961 aacacgggat cataaccctg gtcgatcagg aacttgagca cctcatcgga gagcaaaagt
     1021 tccatcttct tttccttgag ccgatccttg acctcgccga gctcgatatc aatgatttgt
     1081 tcgaggtcct ggcgcgtcaa cgggtgaaag acgatgatgt cgtcgacccg gttcaagaac
     1141 tccggacgaa agactctcct ggtctcatcg aggagcttcg tcttgagatt gtcgtagttc
     1201 agatcgtcct ttttgaccgt aaagccaagg tcgccctgtt tgcggacgag atccgcgccg
     1261 acgttggagg tcataatgac gatcgtgttc cggaagtcca ccttgcggcc gaaagaatcc
     1321 gtcagccggc cttcctccat cagctggaga aggatattaa aaacgtccgg atgcgctttt
     1381 tcgatctcgt caagcaacac caccgaatag gggcgccggc gcacctttgc cgtcaactga
     1441 ccgccctctt cgtagccgac atagccgggg ggcgcaccga cgagacgtga gacgttgaac
     1501 ttctcaccat attcggagca atcgaccgtg atgatggcgt cttcgtcgcc gaacataaac
     1561 tcggcgagag cgcgcgcgag cagcgtcttg ccgacacctg tgggaccgag aaaaataaag
     1621 gtgccgatgg gacggcgcgg attgcggagc ccggaccgcg agcggcggac cgcgtgcgcg
     1681 atcgcccgga tcgcctcgtc ctggccgata atccgtttat ggatttcttc ctccatcttg
     1741 agaagacgcg cggtctcttt ttcttcaaga cgtgcgagcg gaacaccggt ccacttcgag
     1801 accacgaacg cgatatcctc acccgtcacc tggatttcga cctcggattt ggattccttc
     1861 cagctcttct tcatcttttg gaggtcttcc ttggccttgc gttcctcgtc ccggagcttt
     1921 gcggcctttt cgaaatcctg tcccttgatc gaagcttctt tttcacggcg aagcgattcg
     1981 atctcggtct ccattttctt caggtccttc gggagcgtcg tcacggaaag acgggcgcgc
     2041 gaagcggcct catcgataat atcgatcgct ttgtccggaa ggaaccggcc ggaaatatag
     2101 cggtcggaga gtttcgcggc ttcggagagg gcttcatcgg tgatcttgac ccggtgatgc
     2161 gcctcatact tatcgcgaag gcctttcaga atctggatcg tgtcgtcgac cgagggagga
     2221 tcgacgatga cggtctggaa acggcgggcc aacgcggcgt ccttttcgat gtatttacgg
     2281 tattcgtcga gcgtcgtcgc gccaatgcac tggatctcgc cgcgggaaag cgatggcttc
     2341 aagatgttcg atgcatcaat cgcgccttcc gcgccgccgg cccctaccag agtgtgaagc
     2401 tcgtcgatga aaataatcac gttgtcggcg cggcggatct cgtccatcac cgccttgatg
     2461 cgttcctcga actgcccgcg gtatttcgta ccggcaacca tcagagcaag atccagcacg
     2521 atcatgcgct tgtcg
//
LOCUS       DAOOBX010000125         2532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_45419_length_2532_cov_2.924102, whole genome shotgun sequence.
ACCESSION   DAOOBX010000125 DAOOBX010000000
VERSION     DAOOBX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45419_length_2532_cov_2.924102"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="PLY30_02415"
     CDS             complement(<1..1200)
                     /locus_tag="PLY30_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPW64989.1"
                     /translation="MLFHLRNPRTVLRKALTGLILFFLGAVSAYLVSLILPQFVAGTW
                     GTHPLMPVFVMTLISLVAYKPIERFVKWFLEKYLFRRKSYAQMTLMDLAEDLRTNLDL
                     QELANMVVNTFGEVLRLRNVALLVPDATRSGFEIASSFGWPIAVSKRILLACDAPLVQ
                     HIKESGPHVVVRSPLLKSLSWQEANEMGGDFDVLRSGWVVPLFVREDLVGMIAFGAHS
                     PDMVFDESDFHFFREFAEAVAPCVRNAQVVRQLRNTNFQLQDSQAEWFQKTKMSAIEK
                     LAAGIAHEVHNPLAIISGKAQVLLMQKNRAPLEPHIEEALNVMVKQTRRAADITRKLL
                     MYSQESKSPREWFSLGKILDETVSLVGYQASLDRIEISCSVDPELPEFFGNVQEIREI
                     FLNLFFNA"
     gene            complement(1227..>2532)
                     /locus_tag="PLY30_02420"
     CDS             complement(1227..>2532)
                     /locus_tag="PLY30_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPW64990.1"
                     /translation="AWFTHPASEFVPQGTRCPKCGGSEFEKETDIIDVWFDSGVSHQA
                     VLRQYPGLGFPADLYLEGSDQHRGWFQSSLTTAAALEGKSSFKSVLTHGFVVDGQGKK
                     MSKSAGNVVTPQEVMKEFGADVLRLWVASCDYQFDVRLSNDILKQLVETYRKIRNTYR
                     YLLSNLYDFDVTKDAVPFGKQHPIDRWAVDKTDLLVRTVTDLYERFEFHQIYREVYNF
                     CVVDLSAYYLDVLKDTLYTAAPASWLRRSAQTSLYYILARLVKLTAPIMPFTSDEVWK
                     SYVLEPGVPTVHASAWPEPQARQNPEFAAWEHIREIRDAVIPFLEKQREAKVIGAGLE
                     AKVAFTVDLPGSAEILHRYEKELARVFVVSQVSLEAAAVAGVEAAEIVSSVFREKLRL
                     KVLVTRADGAKCVRCWNYSSAVGRDPRHVALCPKCVEAIQSR"
BASE COUNT          579 a          735 c          721 g          497 t
ORIGIN      
        1 cgcgttgaaa aaaaggttca ggaatatctc gcggatctcc tgaacattcc caaagaattc
       61 cggaagctcg ggatccaccg aacagcttat ctcgatacgg tcgagcgacg cctgataccc
      121 cacaagcgac acagtctcat ccagaatctt tcccagcgaa aaccattccc tcggcgattt
      181 ggactcctgg gagtacatga gaagctttct cgttatatcg gccgcccggc gggtttgctt
      241 caccatgacg ttgagcgcct cttcgatgtg gggctcaagg ggagcgcgat tcttctgcat
      301 gaggagcacc tgggccttgc cggaaatgat cgcgagcggg ttatggacct cgtgggcgat
      361 ccctgcggca agcttttcga tcgcggacat ttttgttttc tggaaccact cggcctggga
      421 atcctggagc tgaaaattgg tgttccggag ctgccgtacg acctgggcat tgcgaacgca
      481 gggggcgacg gcttccgcga actcccggaa gaaatggaaa tccgactcgt caaagaccat
      541 gtccggggaa tgcgcgccga aggcgatcat gcccaccagg tcttccctga caaaaagagg
      601 gacgacccaa ccggaccgga ggacgtcaaa atcccctccc atctcgttgg cttcctgcca
      661 ggaaaggctt ttgagcagcg gggatcgcac gacaacatga ggacccgatt cctttatgtg
      721 ctggaccagc ggcgcatcgc acgcgagcag aatccgcttc gacacggcga tcggccatcc
      781 gaacgacgac gcaatctcaa atccgcttcg tgtggcgtcc ggcacgagaa gcgcgacgtt
      841 ccgaagccga aggacttcac cgaacgtatt gacgaccatg ttcgcgagct cctgcaggtc
      901 aaggttcgtc cggagatctt cagccagatc catgagggtc atctgagcgt aacttttgcg
      961 gcggaaaaga tatttctcga gaaaccactt cacgaaccgt tcgatcggtt tgtaggccac
     1021 aagcgagatc agggtcatga caaagaccgg catcagcgga tgggtccccc acgtcccggc
     1081 aacaaactgc gggaggatca gggaaacaag gtaggccgaa acggcaccca ggaagaaaag
     1141 aatcaacccg gtcagggcct tccggagcac ggtcctcgga ttccttaaat ggaacagcat
     1201 gatcgccaac ggattccctc tttcatttaa cgggactgaa tcgcttcaac acacttcgga
     1261 caaagcgcga cgtgccgggg atcgcgaccg acggcggaag aataattcca gcaacgaacg
     1321 catttggcac cgtcggccct ggtcacaagc actttgagcc gcagcttctc ccggaagacc
     1381 gaagaaacaa tttctgcggc ctccacgccc gccacggccg ccgcttccag ggaaacctgg
     1441 gacacgacga aaacgcgagc gagctctttt tcatatctgt gtaatatttc ggcagatccc
     1501 ggcaagtcta cagtaaaagc gacctttgcc tcgagccccg caccgatcac ttttgcctct
     1561 ctctgctttt caaggaacgg tatcacggcg tcacggattt cgcggatatg ctcccacgcc
     1621 gcgaattccg ggttctggcg ggcctgcggc tccggccacg cactcgcgtg gaccgtcggg
     1681 acccccggtt caaggacata ggatttccag acctcgtcag aggtgaacgg catgatgggt
     1741 gcggtcagct tcacgagccg ggcgaggatg taataaagcg acgtttgggc cgaacgccgg
     1801 agccacgaag cgggcgcggc ggtgtagagg gtgtccttca gcacatcgag ataatacgcg
     1861 ctcagatcga ccacgcagaa gttgtagacc tcgcggtaga tctggtggaa ttcgaaacgc
     1921 tcatacagat ccgtcaccgt ccgaaccaga aggtcggtct tgtcaactgc ccagcggtcg
     1981 atcggatgct gtttcccgaa cgggacggca tctttggtca cgtcgaaatc gtagaggttc
     2041 gagaggaggt accggtaggt gttgcgaatc ttgcggtagg tctcgaccag ctgtttcagg
     2101 atatcgttgg aaagacgcac atcgaactgg taatcgcagg aagcgaccca gaggcgcaga
     2161 acatccgcgc cgaattcttt catgacttcc tggggcgtca cgacgttccc cgcggatttc
     2221 gacatcttct ttccctggcc atcgacaacg aatccgtgcg taagaacgct tttgaaggaa
     2281 ctctttcctt ccagcgccgc cgcggtcgta agcgagctct ggaaccagcc gcgatgctgg
     2341 tccgacccct caaggtaaag gtctgccgga aacccgagcc caggatactg acggagcacc
     2401 gcctgatggc tcacgcccga atcgaaccag acgtcgatga tatcggtctc tttctcaaac
     2461 tccgagccgc cgcatttcgg acatcgggtg ccttgtggta cgaattcgga cgcggggtgg
     2521 gtaaaccagg cg
//
LOCUS       DAOOBX010000126         2508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_45964_length_2508_cov_3.514064, whole genome shotgun sequence.
ACCESSION   DAOOBX010000126 DAOOBX010000000
VERSION     DAOOBX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45964_length_2508_cov_3.514064"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLY30_02425"
     CDS             complement(<1..277)
                     /locus_tag="PLY30_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPW64991.1"
                     /translation="MKRTSPGGKQMPGKFKILFVTSEAVPFVKTGGLGDVCGALPKIL
                     KKLGHDVRLVLPRYWAVDRYANNLKTILAPMGVEMGGGTVWCEVLQGK"
     gene            521..1426
                     /locus_tag="PLY30_02430"
     CDS             521..1426
                     /locus_tag="PLY30_02430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPW64992.1"
                     /translation="MESVTSDGTKPLVSVIIPFYNRAHVLPRAVDSVLAQRGCNFELI
                     LVDDGSTDAATPGHGAQSTKYGEKATGRHTVLRAPHSVPVRWISQSNKGPSAARNLGI
                     RHAHGKWIAFLDSDDEWKPGKLKAQMKFFEENPDYRICQTEEIWVRNGVRVNPMKKHK
                     KYGGWIFEKCLPLCVVSPSAVMMHRTLLDEIGLFDESLPACEDYDLWLRIASRYPVGL
                     IEKPYLIKYGGHADQRSREFPAMDRFRIRSLVKLLREGFLTPEQTLAARKTLEEKSRI
                     FIRGALKHGNRKEAGEIQRLIAESL"
     gene            complement(1577..>2508)
                     /locus_tag="PLY30_02435"
     CDS             complement(1577..>2508)
                     /locus_tag="PLY30_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HPW64993.1"
                     /translation="FLAELSRKELIIQGADYDLRMMKKTFGFRPAAPVFDTMLAAQVL
                     GYEKIGLAALVEKFCGVVLPKSSRKADWARRPLLARHLTYAADDTRYLETVAARMTGE
                     LQSLGRLEWHRECCERVVEVSGRPDKAGEREEWRIKGSSKLPSGVLVYVKELWSWRDA
                     ESRRRDRPPFMVMSNENLIALAGWLAANPGASVAGGPAFLRKVSGGTLTRLEDAIRRA
                     RACPAEAFPEPLKMTFTREAPVSGEKVEELRAACKKIADELKIEACFLVSRAAAVDIV
                     RCRPSTVEGVMEAGGLMRWQAERILPAVRAVLG"
BASE COUNT          518 a          805 c          701 g          484 t
ORIGIN      
        1 ccttgccctg gaggacctcg caccagactg ttccgccgcc catttcgacc cccatcgggg
       61 cgaggatcgt cttgagattg ttggcgtagc ggtcgaccgc ccagtaccgc ggcaggacaa
      121 gccggacatc gtggcccagc tttttgagga tcttcgggag ggcgccgcag acatccccga
      181 gcccgcccgt cttcacgaac ggaacagcct cggaggtgac aaagagaatc ttgaacttgc
      241 ccggcatctg cttcccgccg ggggatgttc gtttcacccg agcggtcatt tttttctttg
      301 ttggcataga ttttggaatc atatcacaat atttttctaa aaaaacggaa aatcatcggc
      361 ggaatcgtgc ctcgggggct gtcaacggcc atcctcctcc cttgtgttat aattcgaatt
      421 cgcccgcaga tttcctcaaa accgttaaaa gcgggacccg tgcgtaatca ggagctatca
      481 aaaaaatgac gcctctttct tttgcggaag gcattgcgac atggaatccg tgacctccga
      541 cggaacaaaa ccgctcgtca gcgtcatcat ccccttttac aaccgggcgc atgtcctccc
      601 ccgcgccgtt gattccgtcc ttgcgcaaag gggctgtaac ttcgagctca ttctcgtgga
      661 cgacggatcg acggacgcgg caacgcccgg acacggagcg caaagtacga aatacggaga
      721 aaaagccacc ggccgtcaca ctgtactccg cgccccgcac tccgtgcctg ttcgatggat
      781 ttctcaatca aataaaggcc cttccgccgc gcgcaatctc ggcatccgtc acgcgcacgg
      841 caaatggatc gcgttcctcg attccgacga cgagtggaaa cccggcaagc tcaaggcgca
      901 gatgaagttc ttcgaagaaa atccggacta ccggatttgt cagaccgagg agatttgggt
      961 ccgaaacggc gtgcgcgtca atccgatgaa gaaacacaaa aaatacggcg gctggatttt
     1021 cgaaaagtgc ctgccgcttt gcgttgtgag cccgtcggcc gtcatgatgc accgcacgct
     1081 cctcgacgag attggtcttt tcgacgaatc gctccccgcc tgcgaagatt acgatctctg
     1141 gctccggatc gcctcccgtt atcccgtcgg gctgatcgaa aaaccttacc tcattaaata
     1201 tggcggacac gcggaccagc gctcccgcga attcccggca atggaccggt tccgcatccg
     1261 ctcgctcgtg aaactgttaa gggaaggctt cctcacaccg gaacagaccc tcgccgcccg
     1321 gaaaaccctt gaggaaaaat cccgcatctt catccggggc gcgttaaagc acgggaaccg
     1381 gaaggaagcc ggggaaatcc agaggcttat cgcggagagt ttataacctt tcaccacgcg
     1441 cccggcacca tggaccaggc gcggccactg tttcgcagag tttcgcccgt ttcgcttccg
     1501 ccgtttcggg ctttttcaat aaggttgccg gcccgcgctg ttcttgggcg gaaagttttc
     1561 cggcggggcg tcccggtcag cccagcacgg ccctgaccgc cggcaggatc ctttcggcct
     1621 gccagcgcat gagaccgccg gcctccatga cgccctcaac cgtcgaggga cgacaacgca
     1681 caatatcgac cgcggcggcg cgggagacca gaaaacaggc ttcgatcttc agttcgtcag
     1741 cgatcttctt gcacgcggcg cgcagttcct ccactttttc gccggagacc ggcgcctccc
     1801 gcgtgaaggt catcttcagc ggctcgggaa aggcttccgc gggacaggcc cgcgcgcgac
     1861 ggatcgcgtc ttccagtctc gtgagagtgc cgccgctgac cttccggagg aaggcgggac
     1921 ctcccgcgac agacgcgccc ggattcgcgg caagccaccc ggcgagcgcg atcagattct
     1981 cgttcgacat gaccatgaac ggcggacggt cccggcgccg ggactcggcg tcgcgccacg
     2041 accagagctc cttcacgtag acaagcaccc cggacggcag cttcgaggat cctttgattc
     2101 gccattcctc cctctccccg gccttatccg gacgaccgga aacttcgacg acgcgctcgc
     2161 aacactcccg gtgccattcg agccgaccca gggactgaag ctcaccggtc atccgagcgg
     2221 cgacggtttc gagataccgg gtgtcgtccg ccgcgtaggt cagatgcctg gccaacaagg
     2281 gacgccgggc ccagtcggct ttccgggaag atttcggaag aacgaccccg cagaactttt
     2341 cgacaagcgc cgcgagaccg atcttttcgt aaccaagcac ctgcgcggca agcatcgtgt
     2401 cgaaaacggg cgcggcaggc cggaacccga aggtcttttt catcatccgc aggtcatagt
     2461 ccgcgccctg gataatcagt tccttgcggg agagttccgc gaggaatt
//
LOCUS       DAOOBX010000127         2501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_46152_length_2501_cov_2.812756, whole genome shotgun sequence.
ACCESSION   DAOOBX010000127 DAOOBX010000000
VERSION     DAOOBX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46152_length_2501_cov_2.812756"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /gene="folE"
                     /locus_tag="PLY30_02440"
     CDS             complement(<1..445)
                     /gene="folE"
                     /locus_tag="PLY30_02440"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPW64994.1"
                     /translation="MTKRKSRNQSGDDVLPGLVGQMLVQLGEDPKREGLMRTPIRVAK
                     SLRYLTEGYKTDPKDILAHAVFKEPYDDMVIVKDIEIFSLCEHHLLPFYGKAHVAYIP
                     NGKIIGLSKIPRLVEMFSRRLQVQERLTTQVAESLMDALKPKGVAV"
     gene            complement(507..1535)
                     /locus_tag="PLY30_02445"
     CDS             complement(507..1535)
                     /locus_tag="PLY30_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPW64995.1"
                     /translation="MSTFSHITREKVTECLRRVHDPVLKKDIITLGLVREISVEGAHV
                     FLHMEDASGDATVRGTLKVLIERVLQEAGVTGVEVHFSEKARCGIESGVLGPLSGVKH
                     VIAVSSGKGGVGKSTVAVNLALALRKLGSRVGLMDGDIYGPNIPIMLGIPADRCPEGS
                     EAGKILPIEAHGVKAISIGFFADAGKPVIWRGPMLHKTVQEFLHRVDWGELDFLVVDL
                     PPGTGDVQLSLVQWVRVAGAVVVTTPQEVALADVRRAINMFRQLEVPVLGIVENMTGP
                     VFGRGGGERMAKDFDVRFLGDIPLDAQIRECGDSGIPVVLEQPQGPVSKRFLQIANSV
                     RQVFHKKH"
     gene            complement(1555..2424)
                     /locus_tag="PLY30_02450"
     CDS             complement(1555..2424)
                     /locus_tag="PLY30_02450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPW64996.1"
                     /translation="MKGFLKFTAVFAGILLLSAFLTPILFDFLPFKFERIFNRLVMIG
                     TLLAIVVFVRIRKETLIRFGLIWDKASPGLFAAGFLAGILVLSATSALNVTFGHARFV
                     VPSFSAAEWGSKLLLAFFTGLLIGVIEEFFFRGFVFRQLQGILKGRVVPAVLVTSVFY
                     GLIHFIGMKKIFVGPDPGFSDGLKLIAAPFISLMDWPNFWPEAVGLFLFGIALNSAVC
                     RTGSLYPSIGLHAGCVFFIRLDDLFLKFQGPKTLFWGSKIVYDGMVGWAFLALLAIIL
                     YKGLRPADGTPKA"
BASE COUNT          595 a          801 c          654 g          451 t
ORIGIN      
        1 cgaccgcgac gcctttcggc ttgagcgcgt ccatcaggga ttcggcgacc tgggtcgtaa
       61 ggcgttcctg gacctgaaga cgccgcgaga acatctccac aaggcgcggg atcttggaaa
      121 gcccgatgat cttcccgttc gggatgtagg caacatgggc ctttccataa aacggcagaa
      181 gatgatgctc gcagagcgaa aagatctcga tatccttcac aatgaccatg tcgtcgtagg
      241 gttccttgaa gacggcatgc gcgaggatat ctttcggatc cgtcttgtag ccttccgtca
      301 ggtaccggag cgatttcgcg acacggatcg gcgtccgcat gagtccttcg cgtttcgggt
      361 cctcccccag ctggaccagc atttgcccga ccaaccccgg gagcacgtca tccccgcttt
      421 gatttctgct ctttcttttg gtcatttcgc ctcccatttt gacacccact ctacgcccgg
      481 gagctagctc ccgggcgtag agtgggttaa tgctttttat gaaaaacctg acgaaccgag
      541 ttcgcgatct gaaggaaccg tttgctcaca ggtccttgcg gctgctccaa tacgaccgga
      601 atccccgagt ccccgcattc acggatctgc gcatcaagcg ggatatcgcc aagaaaccgc
      661 acgtcaaaat ccttcgccat ccgctcccca ccgccgcgcc caaacactgg ccccgtcata
      721 ttctccacga tcccgagcac cggcacctca agctgccgga acatattaat cgcgcgccgg
      781 acatccgcaa gcgccacttc ctgcggcgtc gtgacaacca ccgcacccgc cacgcggacc
      841 cactggacaa gcgaaagctg gacatcgccc gttcccgggg ggagatcgac cacgagaaaa
      901 tcgagctcgc cccagtccac acgatgaagg aattcctgga cggtcttgtg gagcatcggc
      961 ccccgccaga tcacgggctt gccggcatcc gcgaagaagc cgatcgagat cgccttcacg
     1021 ccatgcgcct caatcggaag aatcttgccc gcttccgacc cttccggaca acggtcggcc
     1081 ggaataccga gcatgatcgg aatgttcggc ccgtaaatat caccgtccat aagcccgacc
     1141 cgggagccca gtttcctgag ggccagcgca agattgaccg caaccgtgga tttccccacg
     1201 ccgccctttc cgctcgaaac cgcgatcacg tgcttcacac ccgaaagcgg ccccagcacc
     1261 ccactttcga tcccgcacct ggccttttcg gaaaaatgaa cctcgacccc cgtcacaccc
     1321 gcttcctgaa ggaccctctc aataagcacc ttgagcgttc cgcgaaccgt cgcgtctccc
     1381 gaagcgtctt ccatgtgaag aaaaacatgg gccccttcga ccgagatctc ccggacaaga
     1441 ccaagcgtaa tgatgtcttt cttaagtacg gggtcgtgga cgcgccggag gcactccgtc
     1501 accttctcac gcgttatatg gctgaaagtc gacatcgtcc ggggacctcc gccttcaggc
     1561 cttgggggtc ccgtcggccg gacggagccc tttgtagagg attatggcaa gcagggcaag
     1621 aaaggcccat ccgaccatcc cgtcataaac gatcttggat ccccagaaga gggtcttcgg
     1681 cccctggaac ttgagaaaaa gatcgtcgag ccggataaag aacacacagc ctgcgtggag
     1741 accgatcgaa ggataaagcg aacccgtccg gcagacggcg ctgttcaggg cgatcccgaa
     1801 caggaaaagc cccaccgcct ccggccagaa attcggccaa tccatcagcg atatgaacgg
     1861 tgccgcgatc agcttgaggc cgtccgaaaa cccgggatcg gggccgacaa agattttttt
     1921 catgccgatg aaatggatga gtccgtagaa aacgctcgtg acgagaaccg ccggcacaac
     1981 gcgccctttc aggattccct gaagctgcct gaagacaaat ccccggaaga aaaactcctc
     2041 gatcaccccg atcagcaggc ccgtgaaaaa agcgagcagg agttttgaac cccattcggc
     2101 cgcggaaaag gaaggcacga caaatcgcgc gtgtccgaag gtcacgttga gggcgctcgt
     2161 cgcgctcagc accaggattc cggccagaaa accggccgcg aacagccccg ggctcgcctt
     2221 gtcccagatc agaccaaagc ggatgagagt ctccttccgg atccggacaa acaccacgat
     2281 cgcgagcaac gtcccgatca tcacgagacg attgaaaatc ctttcgaact taaacggcag
     2341 gaaatcaaaa aggatggggg tgaggaacgc gctcaaaaga agaatgccgg cgaacaccgc
     2401 cgtaaatttc aagaaacctt tcaccggatg cgtgctcctg ttttaattta ataaaaagac
     2461 attccaccaa agggcctgag agggccaaca ctgccgccgg a
//
LOCUS       DAOOBX010000128         2490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_46420_length_2490_cov_3.404517, whole genome shotgun sequence.
ACCESSION   DAOOBX010000128 DAOOBX010000000
VERSION     DAOOBX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46420_length_2490_cov_3.404517"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PLY30_02455"
     CDS             complement(<1..604)
                     /locus_tag="PLY30_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HPW64997.1"
                     /translation="MKRAVKKPSSRASKTAAKAKSSRASTTSKKAAASVVSPQKANAI
                     VENLLALGKKQGFVTIEQMKKHLPSEMQGADKVEEIIARLAEKNIQVTSESIGEQGKS
                     TMDALQDVDAKKTKDSELEEDGIEEPERVRIDDPVRTYLRQMGQIALLTREQEIAIAK
                     KIEDCEEDLKKAVYQTGAARHEVLEIARKILDGGLQLEEIV"
     gene            complement(783..>2490)
                     /gene="dnaG"
                     /locus_tag="PLY30_02460"
     CDS             complement(783..>2490)
                     /gene="dnaG"
                     /locus_tag="PLY30_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPW64998.1"
                     /translation="LKRSGRNFKACCPFHGEKTPSFMVQPEKQLFHCFGCQVGGDVFS
                     FLMRYENLTFPEALKMLADRAHITLPERRFPKDEASSRKEKIYEIYRVAVDFYQNLFL
                     SDAGKTARAYLAKRGITDETVREFKVGWAPDEWRTLYDHLTKKGYSEALLKQSALIQQ
                     SAKGNYYDTFRGRVLFPIMNLQHKPIALGGRLLADGEGPKYLNSPENPVFSKRRELFG
                     LNLAKKFIDRDWPNLILVEGYMDFLALYQAGFKNTVATLGTALGEDHMRLMKRFVEEV
                     IVVYDGDKAGEAAALRGLEILLEGGIQVKLVSLPKGFDPDDFVRKEGNEAFRALLKDA
                     QDFFDYKLKALVSRYPKHDALGLAKISREMLETFQKIKNPLIRSEYFKRLSRELRVDE
                     DSLRAEFKNMTRRAGGPAAGRSVAEAKPVPKPAAVVVPKEELMLLSLMIDDSDFLERA
                     GDEISKEDFRSPVTRDFFAYLLATPLPEKKKPLTTVLGRIQDAACREQLVGTVAFLET
                     VEDKGQVFADCVRTIRKNRTSSRQEELKRLIHEAELGGDAAKALAYTREFQELLKRPK
                     GG"
BASE COUNT          490 a          742 c          679 g          579 t
ORIGIN      
        1 caacaatctc ttcgagctgg agcccaccgt ccagaatctt gcgggcgatt tccagcacct
       61 catgacgcgc ggcgccggtc tgatacacgg ccttcttgag gtcctcttcg caatcttcga
      121 tcttcttcgc aattgcgatc tcctgctcgc gggtcagaag ggcgatctgc cccatctgcc
      181 gcaggtaggt tctgacggga tcatcgatcc tgacacgctc gggctcttca atgccatctt
      241 cttcaagttc ggaatccttc gtcttcttgg catcgacatc ctggagggca tccatcgtgg
      301 acttcccctg ctcgccgatc gactcgcttg tgacctggat attcttctcc gcaagcctag
      361 cgatgatctc ctcaaccttg tcggcgccct gcatttcgga aggcaggtgc tttttcatct
      421 gctcgatcgt gacaaacccc tgtttttttc cgagggcgag cagattctcg acgatggcat
      481 tggccttttg cggggacacc acggacgccg cggccttttt ggaagttgta gaggccctgg
      541 aggatttcgc cttggcggct gttttggacg ccctggagga aggtttcttg actgctcgtt
      601 tcacccggat tgatctccga tttctaagga ttattgggga aaaacaccgt gtccgcaccc
      661 atcccgaaca gacctcacac gcaaaagaat gacactgagt ccaacgggga ttataggatc
      721 ggatattata gttatgccgg gaaggaatac aaacgatatt tgcaacgcgg gcgaccgggc
      781 ggtcaccccc ctttgggacg ctttagaagc tcctggaact cccgtgtata ggcgagcgcc
      841 ttggcggcat ccccccccag ttccgcctcg tggatcaggc gtttgagctc ttcctggcgg
      901 ctgctcgtcc ggttcttccg gatcgtccgg acgcagtccg cgaagacctg ccccttgtct
      961 tcgaccgttt caagaaacgc caccgtcccc accagttgct cgcggcaggc ggcgtcctgg
     1021 attcttccga gcacggtcgt gagcggcttc tttttctcgg ggagcggcgt tgcgagcaga
     1081 taagcgaaaa aatcgcgcgt gacgggtgag cggaagtctt ccttggagat ctcgtccccc
     1141 gctctttcca gaaagtcact atcatcgatc ataagcgaca ggagcatgag ttcttccttg
     1201 ggcacaacga ccgccgccgg tttgggtacc ggtttggcct cagcaacact gcgccccgcc
     1261 gccggaccgc ccgcgcgccg ggtcatattc ttgaattcgg cacgaagcga atcttcatct
     1321 acccggagct cacgcgagag ccgcttgaaa tactccgacc ggatcaaggg attcttgatc
     1381 ttctggaagg tctcgagcat ttcccggctg atcttcgcga gacccagcgc gtcatgtttg
     1441 ggatagcggc tgacgagggc tttcaacttg taatcaaaaa aatcctgcgc gtccttgagc
     1501 agcgcgcgga aggcctcatt gccttctttc cgcacaaagt catcaggatc gaatcccttc
     1561 ggaaggctga caagttttac ctggatccct ccttccagaa ggatttcgag cccgcgcagc
     1621 gccgcagctt caccggcctt gtccccgtca tagaccacga tcacttcctc cacgaagcgc
     1681 ttcatgagcc gcatgtggtc ctccccaagc gccgtcccga gggttgcgac cgtgttcttg
     1741 aaacccgcct gatagagcgc gagaaaatcc atatagcctt cgacaaggat gaggttcggc
     1801 cagtcgcggt cgatgaactt tttcgcgaga ttcagtccga aaagttcccg ccgtttggag
     1861 aatacgggat tctccggaga attcagatat ttggggccct ccccatcggc aagcaaacgt
     1921 ccgcccaacg cgatgggttt atgctggaga ttcatgatcg ggaagagcac gcggccgcgg
     1981 aaggtgtcgt aataattacc tttcgcggac tgctgaatga gcgccgactg tttgagcagc
     2041 gcctccgaat aacctttctt cgtcagatgg tcatagagcg tccgccattc gtccggtgcc
     2101 cagcccacct tgaactcccg gacggtctcg tccgtgatgc cccgtttcgc gaggtaggcg
     2161 cgcgctgttt ttccggcgtc gctcaaaaaa agattctgat aaaaatcgac cgccacccga
     2221 tagatttcgt atatcttctc tttccgggaa gaggcttcat ccttggggaa ccggcgttcg
     2281 gggagcgtga tgtgagcgcg gtccgcaagc atcttgagcg cctcgggaaa agtgagattc
     2341 tcataccgca tcaggaagct gaacacgtcc ccgcccacct ggcagccaaa gcagtggaaa
     2401 agctgttttt cgggctggac cataaaggac ggggttttct ccccgtggaa cggacagcag
     2461 gccttgaagt tgcggcccga acgcttcagg
//
LOCUS       DAOOBX010000129         2489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_46454_length_2489_cov_2.655300, whole genome shotgun sequence.
ACCESSION   DAOOBX010000129 DAOOBX010000000
VERSION     DAOOBX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46454_length_2489_cov_2.655300"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLY30_02465"
     CDS             complement(<1..550)
                     /locus_tag="PLY30_02465"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPW64999.1"
                     /translation="MNVPSRLREFNVSGRGLSPREKEAVRAEFLRAKVPPAALAIWDY
                     KKHFRIGCYGLARAKEKCIVEGLKSAGIRGFHVRGRSLAPGDWFYKWRETCRVMTAGR
                     KFLIVPAWRKAPRTRSKRIPVILDPGSAFGTGGHETTQLMVRLMEGLEGRFRSFLDAG
                     TGSGVLAIVAAKLGAVIVAGFDN"
     gene            complement(558..1364)
                     /locus_tag="PLY30_02470"
     CDS             complement(558..1364)
                     /locus_tag="PLY30_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPW65000.1"
                     /translation="MDLKPIVTAWFFISMALTGPIPRSDALPAEAEAEDYKVLKEIIL
                     LEFSGEKPHETGPLVPGVKTRIRSSDKVMAVTFNACGRTGGEFDSALLDLLEKESVPA
                     TFFVSGIWLEKNPELAKRIAATPLFEIANLGLEARSCLVAPGTGADAPATRNVEEVFA
                     EIEKNARKIEAVTGALPHFFRSAPSHYDDIAVRIARALGYDVVGTGIRPENSTRGEFA
                     GALLRAAAGSIATFSASDAGTRTSILLRETIPQLRAKGFRFVKLRDYPLE"
     gene            complement(1372..1962)
                     /locus_tag="PLY30_02475"
     CDS             complement(1372..1962)
                     /locus_tag="PLY30_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HPW65001.1"
                     /translation="MIYLASRSARRREILRKMKLRFRCVPSRYREKVLPGHGPRELVL
                     KHAFGKALRARLPAKARYVLAADTLVWRKKVYGKPRTMREATRMLTTLSGKAHEVYTG
                     VVLRDLKTGRSFSGTARTRVYFKRLSRDTIRDYFRQVNPLDKAGAYAIQEGPKIVRKI
                     EGSCTNVVGLPVELLRRLLKKCSGQPILKRAKPRRK"
     gene            complement(1959..>2489)
                     /locus_tag="PLY30_02480"
     CDS             complement(1959..>2489)
                     /locus_tag="PLY30_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HPW65002.1"
                     /translation="RPAFMIRLVLAATLSPSYGIYSGFELCENRAKAPGSEEYLDSEK
                     YQYKVWDWDRPGNIKELVGQVNRLRRENPALQDYDNLEFYDTKNDMILCYGKRTPDLS
                     NIIITVVNLDPFHPQEDFVTLPVWKFDIEEWQTYQMRDLLTGEKYYWKGSRNYVRLDP
                     HTCPAHVFLLKKQAGG"
BASE COUNT          477 a          776 c          727 g          509 t
ORIGIN      
        1 cgttatcgaa tcccgcgaca atcacggctc cgagcttcgc cgccacgatc gcgagcacac
       61 cgctgccggt ccccgcgtcg agaaagctcc ggaacctccc ttccaggcct tccatcagcc
      121 ggaccatcag ttgggtcgtc tcatggccgc ccgtcccgaa cgcgcttccg gggtccagaa
      181 tgaccgggat ccgtttcgag cgggtgcgcg gcgccttcct ccacgccgga acgatcagga
      241 atttcctgcc cgcggtcatg acccggcagg tttcgcgcca tttgtaaaac cagtcccccg
      301 gagcgaggga ccgtccccgg acatgaaatc cccggattcc ggcggacttg agcccttcca
      361 cgatacattt ttctttcgca cgcgcgaggc catagcaacc gatccggaaa tgttttttat
      421 aatcccagat cgcgagagcc gccggcggga ccttcgcgcg gaggaattcc gcgcggaccg
      481 cttctttttc ccggggactg agtccgcgac ccgaaacatt gaattcgcgc aatcgggaag
      541 gaacgttcat aagcgtctta ttcgagagga tagtcacgga gtttcacgaa ccggaatccc
      601 ttcgccctga gctgggggat ggtctcgcga agcaggatcg aggtccgggt tccggcatcg
      661 cttgcggaga acgtcgcgat cgaccccgcg gccgccctga gcagggcccc ggcgaactca
      721 ccccgcgtcg aattctcagg ccggattccg gtaccgacca cgtcatagcc gagggccctc
      781 gcgatcctca ccgcgatgtc gtcatagtga gacggcgccg acctgaaaaa atgcggcagg
      841 gcgcccgtga ccgcttcgat cttccgcgcg ttcttctcga tctcggcaaa cacctcttcc
      901 acgttccggg tcgccggcgc atcggcgccg gttccggggg ccacgaggca ggaacgggcc
      961 tcgagaccga gattcgcgat ctcaaagaga ggggtcgccg cgatccgctt cgccagttcc
     1021 ggattctttt caagccagat gccgctcaca aagaacgtcg cgggaacgct ttccttttcg
     1081 agaaggtcca gaagagcgct gtcgaactcc ccgcccgtgc ggccgcaggc attgaaggtc
     1141 accgccatca ccttatcgga gctccggata cgggtcttga cgccgggaac gagaggaccc
     1201 gtttcatggg gcttttctcc cgagaattcg agaaggatga tttctttaag aaccttgtaa
     1261 tcctcggcct cggcttccgc gggaagagcg tcggaacggg gaatgggacc ggtcagcgcc
     1321 atcgatatga aaaaccatgc cgttacgatc ggtttcaggt ccataagctg gtcattttct
     1381 ccggggtttt gccctcttga gtatcggttg tcccgaacat tttttgagca gtctccggag
     1441 caattcgacg ggaagcccga ccacgttcgt gcaggatccc tcgatcttcc gaacgatctt
     1501 cggcccctcc tggatggcgt aagcgcccgc cttgtcgagc gggttcactt gccggaaata
     1561 gtcccggatg gtatcgcggg ataatctttt gaaataaacg cgcgtcctgg cggtccccga
     1621 aaaggaccgg cccgttttca ggtcccgcaa gacaacgccg gtatacacct catgggcctt
     1681 ccccgaaagg gtcgtcagca tccgcgttgc ctcgcgcatc gttcgcggct ttccatagac
     1741 tttcttccgc cacaccagcg tatcggcggc gagcacatag cgggccttcg cgggaagccg
     1801 ggcgcggagg gccttgccga aagcgtgttt caggacaagc tcgcgaggtc cgtgtccggg
     1861 caggaccttt tcccggtagc ggctcggaac gcagcggaag cggagtttca tttttcgaag
     1921 gatctcgcgc cgccgcgcgg accgggacgc cagatagatc atccgccggc ctgtttcttc
     1981 aaaagaaaca cgtgggccgg acatgtgtgg ggatccaggc ggacatagtt acgggaaccc
     2041 ttccagtaat acttctctcc cgtcaaaagg tcccgcatct gatacgtctg ccattcctcg
     2101 atatcgaact tccacaccgg cagtgtcacg aagtcttcct ggggatggaa cggatcgagg
     2161 tttacgaccg tgatgatgat attggaaagg tccggagtcc ttttcccgta gcagaggatc
     2221 atgtcgttct tcgtgtcgta gaactcgaga ttgtcataat cctggagcgc ggggttctcg
     2281 cgacgaaggc ggttcacctg cccgaccagc tccttgatgt tccccggccg gtcccagtcc
     2341 cacaccttgt actggtattt ttcggaatcg aggtattcct cggaacccgg ggccttcgcg
     2401 cggttctcgc aaagctcgaa accgctgtag atgccgtagg acggagagag cgttgccgcg
     2461 aggaccaacc ggatcatgaa cgccggccg
//
LOCUS       DAOOBX010000130         2472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_46902_length_2472_cov_2.566818, whole genome shotgun sequence.
ACCESSION   DAOOBX010000130 DAOOBX010000000
VERSION     DAOOBX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46902_length_2472_cov_2.566818"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PLY30_02485"
     CDS             complement(<1..872)
                     /locus_tag="PLY30_02485"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPW65003.1"
                     /translation="MSQSLLRDPRFAIFRDLTVGVVRGGTSAERPISLRSGRAVLNAL
                     RRVGVRSVPIDPADAKGFREKVRGVDVLFLALHGNGGEDGVLQATLQRKGIAFTASGA
                     EACRDSFNKLVSKRHFERLGIPTPEYIVVSRKDWKKKTCRFPTPFFVKPLDGGSSQGV
                     FLVEDFPRSAVMIERSVKRYGRLLIEKRIAGREVTAGILVDLKLPVIEVRPKRAFYDY
                     KAKYTKGMTEYIVPAPVPAKVAARVQRLAWKVHRGLGLAGFSRVDFMLDEKFRPYVLE
                     VNSIPGMTELSLLP"
     gene            complement(926..2329)
                     /gene="murC"
                     /locus_tag="PLY30_02490"
     CDS             complement(926..2329)
                     /gene="murC"
                     /locus_tag="PLY30_02490"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPW65004.1"
                     /translation="MTRIVSDDLVKDCREAYLIGIGGSGMSGLARILKHLGLSGGGSD
                     YKESCVTRNLRHDGIRVSIGQKDIGLGGADLVIYSSAIRPDHLELAEARKSGRKVYHR
                     AEVLASLMNHADTAIAVLGTHGKTTTSAMTSFVLSRIGAEPTCFVGSDMINFGTNIVA
                     GGRKFWVSEVDESDSSHEFYAPHYAIIANLEPEHLDHYQSWENLQNSFRRFLAQIHHP
                     GLVTYFGNDPVLSQLVRESGKPSASFGFTEDCDFAAQNVKPLPFGTEFDLHEAGFFSR
                     KVTLSVPGRHNVANALAVIGLLSQMGFELDSICGALREFKGTKRRMEVKWQSSQFIVL
                     DDYAHHPTEVKAVLKALRETGKYVRVIFQPHRFSRTYLFYRDFAHAFDDAHEVLLTDI
                     YAAGEPNPHGVSVELIANEVASAGHPNVRIVHRDRVIPYLSEHPLADGVMVFMGAGDI
                     GDLANDFVGNMNAHPSA"
BASE COUNT          565 a          771 c          644 g          492 t
ORIGIN      
        1 ttggggagca ggctaagttc ggtcattccg ggaatgctgt tcacttcaag aacataaggc
       61 cggaattttt catccagcat aaaatccacg cgggagaaac ccgcaagtcc cagcccgcga
      121 tgaaccttcc acgccaaccg ctgcacacgt gccgccacct tcgccgggac aggcgcgggc
      181 acgatatatt ccgtcatccc cttggtgtac ttggctttat agtcataaaa agcacgcttg
      241 ggccgcacct cgataacggg aagcttgaga tccaccagga ttcccgcggt cacctcgcgt
      301 cccgcgattc tcttttcgat caacaggcgg ccgtaccgct tgaccgaccg ctctatcatt
      361 acggccgaac gcggaaaatc ttcaaccagg aaaaccccct ggctcgatcc gccatcaagg
      421 ggcttcacaa aaaagggtgt cggaaagcgg catgtctttt tcttccagtc cttgcgagaa
      481 acgacgatgt actcgggcgt cggtattccg agcctttcga aatgacgctt cgaaaccagc
      541 ttgttaaaac tgtcgcgaca ggcctccgcg ccggacgccg tgaaggcgat gccttttcgc
      601 tgcagcgtcg cctggagaac gccatcctcg ccgccattgc cgtggagcgc gagaaacagc
      661 acatcgacac cccgcacctt ttcccggaaa cctttcgcgt cagccgggtc gatcggaacc
      721 gatcttactc cgacgcggcg caaagcgttc agcaccgcgc ggccggatcg gagtgatatg
      781 ggccgttccg cggaagttcc gccgcgaacc acacccaccg tgagatcacg gaagatcgca
      841 aatcgaggat cacgcagcag cgactgagac atctggaata attccctgca tcgtttagtg
      901 gatcatgacg aaacggaccg gaggatcacg ccgagggatg ggcgttcata ttgccgacaa
      961 aatcattcgc caaatccccg atgtcccccg cccccatgaa caccatgacg ccatccgcaa
     1021 gcggatgctc cgaaagatac ggaatgaccc tgtccctgtg aacgatccgt acattcggat
     1081 gtcccgcgga ggccacttca ttggcgatca actccacgct gacgccgtga gggttcggct
     1141 cacccgccgc atagatgtcc gtcagaagga cttcgtgggc atcatcgaag gcatgcgcga
     1201 aatcccggta aaaaagatac gtccggctaa accggtgggg ctgaaagatc acgcgaacat
     1261 actttcccgt ttcacgaagg gccttgagca cggccttcac ctccgtcgga tggtgcgcgt
     1321 aatcatccag gacgatgaac tgcgaggact gccatttaac ttccatgcgc cgtttcgtcc
     1381 ccttgaattc ccgcaacgcc ccgcagatcg aatcaagttc aaaccccatt tgggaaagca
     1441 gcccgatcac cgcgagcgcg ttcgcgacgt tgtggcgccc cggcaccgac agtgtgacct
     1501 tccggctgaa gaaaccggcc tcatggagat cgaattcggt accaaacggc aggggcttca
     1561 cattttgcgc ggcaaaatcg caatcctccg taaaaccaaa acttgccgac ggtttgccgc
     1621 tttcacgaac aagctgcgaa aggaccggat cgttgccgaa ataggtcacc aggcccggat
     1681 gatggatctg cgcgaggaac cgccggaacg aattctgaag gttctcccag ctttgatagt
     1741 ggtccaggtg ctccggttca aggttcgcga tgatcgcgta atgaggagcg taaaactcat
     1801 gcgaactatc gctttcatcc acctcagaaa cccagaactt acgccccccc gcaacaatat
     1861 tcgtcccgaa gttgatcata tcgctgccga caaagcacgt cggctccgct ccgatcctcg
     1921 agagcacaaa ggaagtcatc gcgctggtcg tcgtcttgcc gtgggtgccg agaaccgcga
     1981 tcgcggtatc ggcgtgattc atgagggaag caagcacttc ggcgcggtga tacaccttcc
     2041 gtccggattt cctggcctcc gcaagctcga gatgatcggg acggatcgcc gaagaataaa
     2101 tcacgagatc cgcgcctccc agaccgatgt ctttctgtcc gatgctgacg cgaatgccgt
     2161 catgacggag attacgcgtt acgcaagatt ccttgtaatc agaacccccg cccgagaggc
     2221 cgagatgttt caggatgcga gcaagcccgc tcattccgga cccgccgata ccgatcagat
     2281 atgcttcgcg gcaatctttc accaggtcat cactgacgat tcgtgtcatt ttttgatcaa
     2341 ctccgatgcc atcctcgcca actccatgcc ggcctgaggt ctcgcgagcc ccttcatggc
     2401 ctgggtcatg gcatccagtt tcaacgcccc tgcaaggaca tccctcagtt tcgatttaag
     2461 ccagcccgcc gc
//
LOCUS       DAOOBX010000131         2456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_47328_length_2456_cov_3.088713, whole genome shotgun sequence.
ACCESSION   DAOOBX010000131 DAOOBX010000000
VERSION     DAOOBX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47328_length_2456_cov_3.088713"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /locus_tag="PLY30_02495"
     CDS             <1..1699
                     /locus_tag="PLY30_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW65005.1"
                     /translation="SLYGELTVRQNLVLDALLYHIPKQKAETRIAELVEKFGLGPHLD
                     SLADSLPMGLKQRLSLAVAVLHEPKILILDEPTSGVDPVARDSFWELLIELSRKQGVT
                     IFITTHFMNEGMRCDRISLMNAGKVLACDAPQKLIETRGAGDLEEAFIGYMEDAVAET
                     PVESNQPGNVPAEISPKAGEGQTASGVSPWKLRFGRMIAYARNEAMQILRDPVRLIFS
                     FVGSALLMLVFGFGITTDIENIRYASYDLDQSPESRAYLEQFAGSKPYFLPTHPVRSA
                     REALERLQADQVSVVLEIPPNFGRDLRKNDQPQVLAEVDAAMTFRGETVAQYVQAVHN
                     TMLQNPANGLHLPPLRNTAQFQERYMYNPTFESVYSIVPSIPALLLVLIPAILMAVSI
                     VREKELGSMINFYVTPTGKMEYLLGKQLPYVVIGMLNFAILAAMARFVFGVPVKGSFL
                     LLALTTLLYVTSTTGVGMLISSFTSSQVAAVFVTAILTITPTIQFSGLLQPVSTLMGK
                     ARIVGMIWPTTYYMHSSVGAYTKGLSPGLMFQDIVFLLCCIPVLWVFSMLGLRKQEK"
     gene            1702..>2456
                     /locus_tag="PLY30_02500"
     CDS             1702..>2456
                     /locus_tag="PLY30_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW65006.1"
                     /translation="MRAFLNIFWLGLKELRSLVSDRVMLLFVIYAFTFAIYIQATGTS
                     NEVNNASIAIVDEDVSALSKELFTVFYPPRFQIPKKIDASTIEDAMDRSLYMFVVVIP
                     PRFEEDLKAGRNPEIQINIDATAMQQAGIGTNYIKNIISKRISNYLKRTDEEDSPPIR
                     LIVRKLFNPNGTASWFLSVVAIINQITLLTIVLTGAAVIREREHGTLEHLLVMPLTAF
                     EIAMAKVWANGLVILIATAVSLVTVVGMALKVP"
BASE COUNT          495 a          712 c          699 g          550 t
ORIGIN      
        1 ttccctttat ggggaactca ccgtccggca gaatctcgta ctcgacgcgc tcctttatca
       61 tattccgaaa cagaaagcgg agacgcggat tgcggagctt gtcgagaaat tcgggctcgg
      121 gcctcacctc gattcgctgg ccgactcgct tccgatgggc ctgaaacagc gtctctcgct
      181 cgcggttgcc gtgctccatg aaccgaaaat cctgatcctc gatgaaccca cctcaggggt
      241 cgatccggtc gcccgcgaca gcttctggga gctcctgatc gagctctcgc gcaaacaagg
      301 ggtaacgatc ttcattacga cgcatttcat gaacgaaggg atgcgctgtg accggatctc
      361 ccttatgaac gccgggaagg tgctggcctg tgacgctccg cagaaattga tcgaaacgcg
      421 cggcgccggg gaccttgagg aagcgttcat cggttacatg gaggatgcgg tcgcggaaac
      481 accggtcgaa agcaatcaac ccgggaacgt gcccgccgaa atctccccca aagccgggga
      541 ggggcagaca gcctccggcg tttctccctg gaaactccgg ttcgggcgta tgatcgccta
      601 tgcccgcaat gaagccatgc agattctccg ggaccccgtg cggctcatct tttccttcgt
      661 gggatcggcg ctcctgatgc ttgtgttcgg attcggtatt acgactgaca tcgagaatat
      721 ccgctacgcg tcctacgatc tcgaccagtc ccccgagagt cgtgcctatc tcgagcagtt
      781 cgcgggttca aagccttatt tcctgccgac gcatccggtt cggtccgctc gtgaggcgct
      841 ggagcggttg caggcggacc aggtttcggt ggttctcgaa atcccgccga atttcggcag
      901 ggatctccgc aagaacgatc agccccaagt gctggcggag gtggatgcgg ccatgacgtt
      961 tcggggcgag acggtcgcgc aatatgtcca ggcggtccac aataccatgc tgcagaaccc
     1021 cgcgaacggg cttcatctgc ctccactcag gaatacggcg cagttccagg agcggtatat
     1081 gtacaacccc acgttcgaaa gtgtttattc gatcgtgccg agcattcccg cgctgctcct
     1141 cgtcctgatc ccggcgatcc tgatggcggt cagcattgtg cgcgaaaagg aactcggttc
     1201 gatgatcaat ttctatgtca cgcccacggg aaagatggaa tatcttttgg gaaagcagct
     1261 gccttatgtc gtgatcggaa tgctgaattt cgcgatcctg gcggccatgg cccgctttgt
     1321 gttcggggtg ccggtcaaag gcagtttttt gctcctggcc ctgaccacgc tgctctatgt
     1381 cacgtcaacg accggcgtcg ggatgctgat ctcaagtttt acctcgagcc aggttgcggc
     1441 ggtcttcgtg acggcgatcc tcacgatcac gccgaccatc cagttctcgg gtctgctgca
     1501 acccgtgtcg accctgatgg ggaaggcgcg cattgtcgga atgatctggc cgaccaccta
     1561 ttacatgcat tcgagcgtcg gggcttacac gaaaggtctc agtcccggtc tcatgtttca
     1621 ggatatcgtc ttcctgcttt gctgcatccc ggtcctctgg gtattcagca tgctgggttt
     1681 gaggaaacag gagaaataac gatgagggca ttccttaaca tcttttggct gggactcaag
     1741 gaacttcgca gtctcgtcag cgaccgggtg atgctccttt ttgtgatcta cgccttcacg
     1801 ttcgcgatct acattcaggc gacaggaact tcgaacgagg tcaataacgc gtcgatcgcc
     1861 atcgtggacg aagacgtctc ggccctttca aaagaactct ttacggtttt ttaccctccc
     1921 cggttccaga ttccgaagaa gatcgatgcg agcacgatcg aggatgcgat ggaccggagt
     1981 ctctacatgt tcgtcgtcgt gatcccgccc cgtttcgagg aagacctcaa ggcgggacgc
     2041 aatcccgaga ttcagatcaa tatcgacgcg acggccatgc agcaagccgg gatcggcacg
     2101 aattacatca agaacatcat cagcaaacgg atctcgaact atctgaaacg cacggacgaa
     2161 gaagactccc cgccgatccg gctgatcgtc cggaaacttt tcaatccgaa cggtacggct
     2221 tcctggttcc tgagcgtggt cgcgattatc aaccagatca cgctgctcac gatcgtgctg
     2281 acgggcgcgg cggtgatccg cgagcgcgag cacggaacgc tcgagcatct gctcgtcatg
     2341 ccgcttaccg cgttcgaaat cgcgatggcg aaggtgtggg ccaatggtct tgtgatcctg
     2401 atcgcgacgg cggtttctct cgtcactgtg gtcggcatgg cgctgaaagt cccgtt
//
LOCUS       DAOOBX010000132         2413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_48422_length_2413_cov_3.408397, whole genome shotgun sequence.
ACCESSION   DAOOBX010000132 DAOOBX010000000
VERSION     DAOOBX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48422_length_2413_cov_3.408397"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /gene="ssb"
                     /locus_tag="PLY30_02505"
     CDS             <1..300
                     /gene="ssb"
                     /locus_tag="PLY30_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPW65007.1"
                     /translation="LAEICNEYLKKGRPVFVEGRIQTRSWDGPDGSKRYSTEVVADNI
                     QFLGSKPGGKSESADVPTMDIDNTIFEAPEGSGSSRKGGPSVSQDELKPDEDIPF"
     gene            410..613
                     /gene="rpsR"
                     /locus_tag="PLY30_02510"
     CDS             410..613
                     /gene="rpsR"
                     /locus_tag="PLY30_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPW65008.1"
                     /translation="MRMVRKRVRRVLPPLDYKDTDALSKYLTEKGKILPRRITRLNAK
                     DQRTLKRLIKRARHAGLISFQAI"
     gene            628..1074
                     /gene="rplI"
                     /locus_tag="PLY30_02515"
     CDS             628..1074
                     /gene="rplI"
                     /locus_tag="PLY30_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPW65009.1"
                     /translation="MELLLIKDVPEVGKKGDVVRVRDGFGRNFLLPRNLAILATRKNQ
                     DFFADQKARSEIRKAKEKAAAEKKARELADLKIAIEVKAGEGNKLYGSVGPDEIRQAL
                     SAKGYEFDKKQVRLKEHLKSLGTHQVDLEIYPQVKTRLAVELVRQP"
     gene            1100..>2413
                     /gene="dnaB"
                     /locus_tag="PLY30_02520"
     CDS             1100..>2413
                     /gene="dnaB"
                     /locus_tag="PLY30_02520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPW65010.1"
                     /translation="MANPETTVKLYEKIPPQNREAEMSVLGAMFFDETALIKAIEVLR
                     PACFYDLRHQNLFQVIADLFEKNQPVDLITVSEELRKRKQLDDIGGISYLTQLSTSVP
                     TSANVEYYARIVKEKALLRQLIHSATQIVQNSLESDSNAKEMIDTAEKMIFDIGQSQV
                     EGRSVQIKDIIHENMETIDRLFQKKEYVTGLATGFHEFDTMTAGLQPSDLIIVAGRPS
                     MGKSAFATAIVEHAGINMKKPVVIFSLEMSKEQLVQRMLCSRARVDAQKVRTGYLSHQ
                     DWPKLTAAAGKLSEAPIFIDDSPAQTVLEIRAKARRLKMKHDIQLIVIDYLQLMQGVR
                     SVESRQQEISEISRSLKALAREIRVPVIAVSQLSRAVEQRTGNRPQLSDLRESGAIEQ
                     DADLVVFLFREEYYAPSEENKNKAEAIIAKQRNGPVGSVPLVFIKE"
BASE COUNT          576 a          686 c          699 g          452 t
ORIGIN      
        1 ctggccgaga tctgcaacga atacctgaaa aaaggccgcc ccgttttcgt ggaaggcagg
       61 attcagaccc gtagttggga cggcccggac gggagcaagc ggtattcaac ggaagtggtt
      121 gctgacaaca tccagtttct cggttccaag ccgggaggca agagcgagag cgcggatgtg
      181 ccgacgatgg acatcgacaa cactattttt gaagcaccgg aaggttcggg ctcttccagg
      241 aagggtgggc cttcggtgtc gcaggatgaa cttaaaccgg atgaagatat ccccttttaa
      301 ccaagtgtga gccatggaaa gaaaacccga aggaaaattc gaaaagaaac ctttcaagaa
      361 accgatgagg aaatccggcc gcgacggtga tcgccggggc cccgggggtc tgagaatggt
      421 gcgcaagcgt gtgcgccgcg tcctgcctcc cctcgattac aaggataccg acgcgctttc
      481 gaagtatctc acggaaaaag gcaagatcct gcctcgcagg atcacccggc tcaacgccaa
      541 ggaccagcgg acgctgaagc ggcttatcaa gcgtgcccgg cacgcgggcc tgatctcttt
      601 ccaggccatt taacgaggtg atgtgacatg gaacttttgc ttatcaagga tgtccctgaa
      661 gtcggtaaaa aaggcgatgt ggtgagggtt cgcgacggtt ttggccgcaa cttcctcctg
      721 ccccgcaacc tggcgatcct cgcgacgcgc aagaaccagg atttcttcgc ggaccagaag
      781 gcccgcagtg aaatacgcaa ggcgaaggaa aaggccgcgg ctgaaaaaaa ggccagggag
      841 ctggccgacc tcaagatcgc gatcgaggtc aaggccgggg aaggcaacaa gctttacggt
      901 tcggtcggcc ccgacgaaat ccgccaggcg ttgagcgcga agggttatga gttcgacaag
      961 aagcaggttc gcctgaaaga gcatctcaag agcctgggaa cccaccaggt ggacctggaa
     1021 atctacccgc aggtcaagac acgcctggct gtcgagctgg tccgtcagcc gtaatcccga
     1081 agttttaaac gaggtctttg tggcgaatcc cgagacaacg gtcaagcttt acgagaagat
     1141 cccgccccag aaccgtgaag ccgaaatgag cgtgctcggc gccatgttct tcgacgagac
     1201 cgctctcatc aaggccatcg aggtcctccg cccggcctgt ttttacgacc tgcgccatca
     1261 gaatctcttc caggtcatcg cggatctttt cgaaaagaac cagcccgtgg acctcatcac
     1321 cgtttctgaa gagttgcgca aacgcaagca gctcgatgac atcggcggca tcagctatct
     1381 gacgcaactt tcgacctccg tgccgacctc ggcgaacgtc gagtattacg cccggatcgt
     1441 caaggaaaag gctctcctcc gccagctcat ccattccgcc acgcagatcg ttcaaaacag
     1501 cctcgagtcc gactcgaacg cgaaggagat gatcgacacg gcggaaaaga tgatcttcga
     1561 tatcggtcaa tcgcaggtcg aaggcaggtc ggtccagatc aaggatatca tccacgagaa
     1621 catggaaacg atcgaccggc tcttccagaa aaaagaatat gtgaccggtc tcgcgacggg
     1681 gttccatgag ttcgatacga tgaccgccgg ccttcagcca tcggatctca tcattgttgc
     1741 cgggcgtccc tcgatgggga aatcggcgtt cgcgaccgcg atcgtcgagc acgccggcat
     1801 caacatgaaa aagccggtcg tgatcttctc gctcgaaatg agcaaggagc agctcgttca
     1861 gaggatgctc tgctcccgcg cccgcgtgga cgctcaaaag gtcaggacgg ggtacctctc
     1921 gcaccaggac tggccgaaac tgacggccgc cgccggaaag ctttccgaag cgcccatctt
     1981 cattgatgat tcccccgccc agaccgtgct tgagatccgg gccaaggcgc gccgtctcaa
     2041 gatgaagcat gacatccagc tgatcgtgat cgactatttg cagctgatgc agggcgttcg
     2101 ttccgtcgaa agccgccagc aggagatctc ggaaatctcc cggtccctca aggcgctcgc
     2161 gcgtgaaatc cgggtgcctg tcatcgcggt cagtcagctg tcgcgtgccg tcgaacagcg
     2221 aacgggcaac cgtccccagc tttcggacct ccgcgagtcg ggcgcgatcg aacaggatgc
     2281 tgatcttgtt gtttttctct tcagagagga atattatgct ccttccgaag agaacaagaa
     2341 caaggccgag gcgatcattg ccaagcagcg taacgggccg gtgggctctg tgccgcttgt
     2401 gtttatcaag gaa
//
LOCUS       DAOOBX010000133         2410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_48503_length_2410_cov_3.371975, whole genome shotgun sequence.
ACCESSION   DAOOBX010000133 DAOOBX010000000
VERSION     DAOOBX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48503_length_2410_cov_3.371975"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLY30_02525"
     CDS             complement(<1..204)
                     /locus_tag="PLY30_02525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW65011.1"
                     /translation="MKILQLLPSLEVGGVERGVVDLARALKKEGHEAVVVSSGGSLVS
                     ELHRMGVPHYGLPVHQKSLASLLL"
     gene            complement(305..2254)
                     /locus_tag="PLY30_02530"
     CDS             complement(305..2254)
                     /locus_tag="PLY30_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HPW65012.1"
                     /translation="MEKALDLIAYGIARVLIACFARLPLATSLRIGDAFGKGVFYLSG
                     KRRVAYVNLRNALGERLTPRARWKAVRDHYGLMAQNIVEIMSFPKLTPAHIGQNIRIH
                     GLEKFEALAKSGKGGVLLTGHFGNWELLQVVAGLRGTPIHVLARDQKYPRLNELLNKL
                     RETHGSVAVSRGAGIRSLVRALKEGKLIGVLADQSAGKTEGIILPFFGIKTSVPTGAF
                     ELAVRTGSLLMPCYMVRVEHARHEIFVGDVLRDDPSATNEERIESVARQYLASLEEFI
                     SKYPGQWLWENKRWKYSWSRRILILSDGKAGHFKQSDVVAGMMSSLTEFHDRPGLEFE
                     TVKAHIEYRSAGHRSLLFALAPLMRPWIQGRLNWLAPFLTEESYRAIENARADFIVTA
                     GSSLAPVQQLLARETGAKTVVIMKPPFPYALMPYDLAVVPAHDEGPVPKRTFRSVIMP
                     SGYRAVDRSGDIAALKAKVGDISRIRIAVFLGGKTRGYDIMAPDAEKIAMILRRLSRS
                     GLEYMVTTSRRTTKNVEQFLKNVLSRDPACRYLVVANEENPPYAAGGMMGLADLIIVT
                     EDSLAMISEAVGSGKRVIVLVAGQGDLPAKHYRFHQTLSQRGLVTLCCLEDLERTVAE
                     LLNKAASPVIDREKNALLQKLGELL"
BASE COUNT          475 a          768 c          694 g          473 t
ORIGIN      
        1 gaggagcaat gacgcgagcg atttctgatg cacgggaagg ccgtaatgcg ggacgcccat
       61 gcggtggagt tccgacacaa gcgaaccgcc ggaagaaacg acgaccgctt cgtgcccttc
      121 ttttttcagc gcgcgggcga ggtccaccac cccgcgttcg acgcctccga cttcaagact
      181 cggcaacaac tggaggatct tcatctgttc agagacacgt cagcgcatct ctcctctttt
      241 ttgaacggcc ggtattttca agcaacataa atcctgcggc ggtcctgcgg acggcaggac
      301 ctgtttataa caattcgccc agtttctgca acagggcatt cttttcccgg tcgatcaccg
      361 gggaggctgc cttgttcaga agttccgcga cggtcctctc caggtcctcg agacagcaaa
      421 gcgtgacgag accccgctgg ctcagcgtct ggtgaaagcg gtaatgtttc gccggaagat
      481 cgccttgccc cgccacaagc acgatgaccc tctttccgct tccgaccgcc tccgagatca
      541 tcgcgaggct gtcctccgtc acgatgatga ggtccgcgag ccccatcatc cctcccgccg
      601 catatggcgg gttctcttcg ttcgcgacca caagatagcg gcacgcgggg tcccgggaaa
      661 gcacgttttt cagaaactgt tcgacgtttt tggtcgtccg ccgcgacgtc gtcaccatgt
      721 attccagacc ggagcgggac aggcgccgca ggatcatcgc gattttttcg gcgtccgggg
      781 ccatgatgtc atacccgcgt gttttgccgc cgaggaagac cgcgatccgg atccgggaga
      841 tatcccccac cttcgccttg agggccgcaa tgtcaccgga acggtccacg gcccgatagc
      901 cgctcggcat aatcacggac cggaaagtcc ttttcgggac cggcccctcg tcgtgcgcgg
      961 ggacaaccgc aagatcgtaa ggcatcaggg catacgggaa cggcggcttc atgataacga
     1021 ccgtcttcgc gccggtctcc ctcgcaagaa gctgctgaac cggggcaaga ctcgatcccg
     1081 cggtcacgat gaaatccgcg cgggcgttct cgatcgcccg gtaactttcc tccgtaagga
     1141 acggcgcgag ccagttcagc ctcccctgga tccacggtct catcagcggg gccagcgcaa
     1201 aaagcaggct ccgatgaccc gcggaacggt attcgatatg ggctttcaca gtctcgaact
     1261 ccagaccggg acggtcgtgg aactccgtga gcgagctcat catcccggcg acaacatcgg
     1321 actgcttgaa gtgcccggcc ttgccgtccg agaggatcag gatcctccgc gaccagctgt
     1381 acttccaccg cttgttctcc cagagccact ggccgggata tttcgaaata aactcctcga
     1441 gcgaggccag gtactgccgc gcgacggact cgatgcgctc ttcgttcgtg gcggacggat
     1501 cgtccctgag gacatccccg acgaagatct catgccgggc atgttccacc cggaccatgt
     1561 agcacggcat cagaagcgag ccggtcctca cggcaagctc gaaggccccc gtcgggaccg
     1621 atgtttttat gccgaagaaa ggaaggatga tcccttccgt tttccccgcc gactggtcgg
     1681 cgagcacgcc gatcagttta ccttccttga gggcccgtac aagactgcgg atacccgcgc
     1741 cgcggctgac cgcgaccgaa ccgtgcgttt cccggagctt gttcaagagc tcgttcaacc
     1801 tcgggtattt ctggtcgcgc gcaagcacat ggatcggtgt tccccggagc ccggcgacaa
     1861 cctgcaaaag ttcccagttc ccgaagtgtc ccgtcaggag cacgcccccc ttgccggatt
     1921 tcgccaacgc ctcgaacttt tcgagaccgt gaatccggat gttctgcccg atatgcgccg
     1981 gtgtcagttt cggaaagctc atgatctcga cgatgttttg ggccatgagt ccgtagtgat
     2041 cgcgcacggc cttccaccgg gcgcgcgggg tcaaacgctc accgagcgcg tttcggaggt
     2101 tcacgtaagc aacgcgccgt ttcccggaaa gataaaaaac gccttttccg aacgcgtcac
     2161 cgatccggag gctggtcgcg agaggaaggc gcgcgaagca ggcgatcagg acgcgggcga
     2221 tcccgtaagc gatcagatcg agggcttttt ccattatcgt ttgatcccca ggagccgcag
     2281 cagacggtcg gtcaggattt cttctcccga acggacccgg atgcgcgccg cgagcacgag
     2341 cgggttcaga accctggaga tcagatcccg cgaacggtaa aaatctttct cggtggtaat
     2401 cacttcctcc
//
LOCUS       DAOOBX010000134         2398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_48830_length_2398_cov_3.414426, whole genome shotgun sequence.
ACCESSION   DAOOBX010000134 DAOOBX010000000
VERSION     DAOOBX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48830_length_2398_cov_3.414426"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLY30_02535"
     CDS             complement(<1..184)
                     /locus_tag="PLY30_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW65013.1"
                     /translation="MKKILIVEDDADFQDIYTLYLQGESHEILRAMNGREGLDCLAKT
                     VPDLIILDLIMPVMDGE"
     gene            complement(198..2093)
                     /locus_tag="PLY30_02540"
     CDS             complement(198..2093)
                     /locus_tag="PLY30_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HPW65014.1"
                     /translation="MQISKRRHHHHHRRRRFHETDKTTAAHGEAAAEPRRSFRVSLRY
                     KLAVPVFVLVFSSFAFVLYTTLNTVRDMMTSYKEARLQTIAEVFAETLRIPMSEGDKK
                     TLKAYISLLAEQTDVEEVRVENPDGEVVGNSNPSARSGERFSCKPDFFGVRRTGRDTY
                     AAVAPVTQGDEILGRLIITFTRIDIENELRKLFAERFMIATVLAILATLIVSGITWLT
                     IRPLFTLQRTARRILAGDLDARAKIRSRDEIQEVGDAFNKVVSRLVQSFEHLRLRTQA
                     LEASEEKYRSIVNEVSDIIFSITPEGELLLLNRGFSGYTREEILAGGLQLLLSMHAPE
                     DAQKFQEALELIVSVHQPIFHLNTRHIHRTHRVDIYYQTSMTPVIDYEGNLRMIQGVM
                     RDVTEIRRVEMMKETLIRDVAHELKTPTAKFIMTTQWLERQMTRSPDGQKFLPTVKTL
                     SENADRLMKTISSIMDLSKIESGMVDVQKQEVNLNDILNLVVNDTRPLAAQHHLTIQT
                     EFAPEPLAMLGDPDMLYRLFVNLVSNAIKFTPDGGSITVTSYRKGQAVCADVRDTGIG
                     IEHDHLEKIFEAFYQRTPSSLGIGVGLTISKELVLLHEGDMWAESDGLGKGMVMKTVF
                     PMFRGAT"
     gene            complement(2147..>2398)
                     /locus_tag="PLY30_02545"
     CDS             complement(2147..>2398)
                     /locus_tag="PLY30_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65015.1"
                     /translation="VREVREELAGEIRPRELICRQDCVCPERTVALFFYFCDWVRGEP
                     QCLDCQDARWVSRAGLRELKFPEGDDGVLRKLIRRNLVR"
BASE COUNT          486 a          690 c          712 g          510 t
ORIGIN      
        1 cctcgccgtc catcacaggc atgatcagat cgagaatgat gagatccgga actgtctttg
       61 cgaggcaatc gagcccttcg cgtccgttca tcgcgcgaag aatctcatgc gattctccct
      121 ggagataaag cgtgtaaata tcctgaaaat ccgcgtcgtc ctccacgatc agtatctttt
      181 tcatcatcgc tctcccttca ggtcgcgccc cgaaacatcg ggaaaacggt cttcatgacc
      241 atccctttcc cgaggccatc gctctcggcc cacatgtccc cttcatggag cagaacgagc
      301 tctttcgaga tcgtgagccc gaccccgatg ccgagggaag acggggtccg ttgatagaac
      361 gcttcaaaga tcttctcaag atggtcgtgc tcgatcccga taccggtatc acggacatcg
      421 gcgcaaacgg cctgcccttt ccggtaggag gtgacggtga tcgacccgcc gtccggcgtg
      481 aacttgatcg cgttgctgac aaggttgaca aaaagccggt agagcatgtc gggatcgccg
      541 agcatcgcga gaggttccgg cgcgaactcc gtctggatcg tcagatgatg ctgcgccgcg
      601 agcggacggg tgtcattcac gacgaggttc aggatgtcgt tcaggtttac ttcctgtttc
      661 tggacatcca ccatgccgga ctcgatcttg gaaaggtcca tgatgcttga tatcgtcttc
      721 atgaggcggt cggcattctc cgagagcgtc ttgacggtcg ggagaaattt ttggccgtcc
      781 gggctccggg tcatctggcg ctcaagccac tgggtcgtca tgatgaattt cgcggtcggc
      841 gtcttgagct cgtgagcgac gtcccggatc agcgtctctt tcatcatttc cacgcgacgg
      901 atttccgtca catcgcgcat aacaccctgg atcatgcgga ggttcccctc gtaatcgatc
      961 acgggggtca tgctggtctg gtaatagatg tcgacgcggt gcgttcggtg gatgtgccgc
     1021 gtattcagat gaaagatcgg ctgatggacg ctcacaatga gctcgagagc ctcctggaac
     1081 ttctgggcgt cttcgggggc gtgcatggac aaaagaagct ggagtccccc ggccaggatc
     1141 tcttcgcggg tatagccgga gaacccgcga ttgaggagga gcagttcccc ttccggcgtg
     1201 atagaaaaga tgatgtcgct cacctcattg acgatggaac gatatttctc ctcggacgcc
     1261 tcgagcgcct gcgtgcgcaa ccgaagatgt tcgaagctct gcacgagacg gctcacgacc
     1321 ttgttgaacg cgtcgcccac ctcctggatt tcatccctgg agcggatctt tgcgcgcgcg
     1381 tcgagatcgc cggcaagaat gcgccgcgcc gtccgctgga gggtgaacag gggccggatc
     1441 gtaagccacg taatcccgga aacgatcagg gtggccagga tcgcaaggac ggtggcgatc
     1501 atgaaccgtt ccgcgaacag tttgcggagc tcgttctcga tatcgatccg cgtgaacgtg
     1561 atgatcaggc ggcccaggat ttcatcgccc tgcgtgacgg gcgccactgc ggcataggtg
     1621 tcacgaccgg tccgccggac gccgaaaaag tccggtttgc aggagaaccg ctcgccggaa
     1681 cgggctgagg gattgctgtt gccgacgact tcgccgtcgg gattttcgac gcggacctcc
     1741 tcgacatcgg tctgttcggc cagaaggctg atataggcct tcagcgtttt cttgtcgcct
     1801 tcactcatcg ggatccggag ggtctcggcg aaaacctcgg caatcgtctg gagacgggcc
     1861 tccttgtaac ttgtcatcat atcgcgaacc gtgttgaggg tcgtgtaaag cacgaacgcg
     1921 aagctcgaaa agaccagcac aaaaacaggg accgcaagct tgtacctcag ggagacgcga
     1981 aacgaccggc ggggttccgc ggcggcctcg ccatgcgcgg cggtcgtttt gtcggtctca
     2041 tggaaccggc ggcggcgatg atggtggtgg tgacggcgtt tagagatctg catagagtca
     2101 ggccattata acggcgaaaa aagttctcat gaagagcatt tttattttaa cgaaccagat
     2161 tcctccggat cagcttccgg agaacaccgt cgtctccctc cgggaatttg agttcccgga
     2221 gtccggcgcg cgatacccac cgcgcgtcct ggcaatcgag gcactgcggt tcgccgcgga
     2281 cccagtcgca aaaataaaag aaaagcgcca ccgttctttc cgggcagacg caatcttgcc
     2341 ggcagatcag ctcccgcggc cggatctcgc ccgcgagctc ttcccggact tcgcgaac
//
LOCUS       DAOOBX010000135         2377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_49337_length_2377_cov_3.566753, whole genome shotgun sequence.
ACCESSION   DAOOBX010000135 DAOOBX010000000
VERSION     DAOOBX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49337_length_2377_cov_3.566753"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLY30_02550"
     CDS             <1..481
                     /locus_tag="PLY30_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65016.1"
                     /translation="SQTLSVTIEADEITQTIPIDPDGPGPLAPFEARVSVEPTIKASI
                     ETLLLSCNAGAMTGVTFLKAFTLFAGGGVSAGWGRSEIAIDSKDPINVSGYLGDLIDT
                     PGTIGVTGTADEYADLFIRPYLAGGLAFSVGAFELAIPITFKPVDAISVAVFFGVRL"
     gene            517..1794
                     /locus_tag="PLY30_02555"
     CDS             517..1794
                     /locus_tag="PLY30_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW65017.1"
                     /translation="MLAALILLPETPCVSAETPVDLPVWPGKTIITAASLEGLFTVSK
                     EAGDTVTGYGGRASFGCVGAFARQLALDASLGYRWLNGPDGPIPFPGVSASGGWLLTL
                     PGPFSVTPFGGLSLEVRAEDFSPETVTSLTGGARLAMLLSGRDYLTLTPSVSIPLSGD
                     AGLSLSFALGLRSEWAWMRPVPDKPPKIRAKPELFSPDGDGTDDETVLALSCADPSLI
                     SQWMVIIYGDKDAVLVRHEGTGRPPRSLVWSGEPDPAELEPGFAYRAVFETVDVLGRS
                     ARGETTVVCDILVIRDGDRYRVRVPDIIFPSDSWELSPRESRKLLETNRKVLERVATL
                     FSHFPSYSLIVEGYANAVYRNNPARYAEEQRTELLPLSQKRAETVRDALIMLGIDSAR
                     INTRAYGGERPKTTANDTEEAWKNRRVEFILER"
BASE COUNT          489 a          771 c          653 g          464 t
ORIGIN      
        1 gagccaaacc ctgtccgtga ccatagaggc cgacgagata acccagacca tcccgataga
       61 tcccgacggt cccggcccgc tcgcgccctt tgaagcgcgc gtctccgtgg aaccgacgat
      121 caaggcttcc attgaaaccc tgctcctttc ctgcaatgcc ggcgcgatga ccggcgttac
      181 tttcctgaaa gcgttcaccc tgttcgccgg agggggtgtg agcgccggct ggggacgctc
      241 cgaaatcgcg attgactcga aagatccgat caatgtatcc ggctacctcg gagacctcat
      301 cgacacgccc gggaccatcg gcgtaaccgg cactgccgat gaatacgctg acctcttcat
      361 ccgtccctac ctggccggcg gcctcgcctt cagcgtcgga gccttcgaac ttgccatccc
      421 gataaccttc aagcccgtcg atgctatcag cgtcgccgtc ttcttcggag tccgcctgtg
      481 aaaacccgac cgcaccgcgt accgccaacc gcgtttttgc ttgcagccct gatcctgctc
      541 ccggaaacac cttgcgtgtc cgccgagaca cccgtggacc tgcccgtctg gcccgggaag
      601 accatcataa ccgccgcctc gctggaagga ttgttcaccg tttcgaaaga agccggcgat
      661 accgtgaccg gatacggcgg acgcgcgtcc ttcggctgcg tcggcgcttt cgcccgccaa
      721 ctcgcccttg acgcctcgct cggctaccgc tggctgaacg gacccgacgg gcccattccc
      781 ttccccggtg tttccgcgtc gggcggctgg ctcctgaccc tgcccggtcc gttctctgta
      841 acacccttcg gcggcttatc gcttgaggtc cgggcggagg atttttcccc cgaaaccgtt
      901 acatccctca cgggcggcgc gcggcttgcc atgctcctct ccgggcgtga ctacctgacc
      961 ctcaccccgt cggtctccat tcccctttcc ggagacgcag gactctcact gtcgtttgcg
     1021 ctcggtctga gaagtgaatg ggcctggatg cggcccgtac ccgacaaacc gcctaaaatt
     1081 cgcgcgaagc cggaactctt ttcgccggac ggagacggga cagacgatga aaccgtcctc
     1141 gcgctttcct gcgccgatcc ctcgctcatc tcacaatgga tggtcatcat ttatggagac
     1201 aaggacgcgg ttctcgtacg ccatgaagga acagggcgtc ctccccgctc gcttgtctgg
     1261 agcggagaac ccgatcctgc agaattggaa cccggcttcg cgtatcgcgc ggtctttgaa
     1321 acagtcgatg ttctgggccg aagcgcccgc ggtgaaacaa ccgtcgtctg cgacatcctt
     1381 gtcatacgag acggcgaccg gtaccgggtt cgcgtacccg atatcatctt cccctccgat
     1441 tcctgggaac tctcgccccg ggaatcgcgc aaactccttg aaaccaaccg gaaagtactc
     1501 gaacgggtcg ctaccctctt ttcgcacttc ccgtcctatt cactgatagt ggaggggtac
     1561 gcgaacgccg tgtaccgcaa caatcccgcg cggtacgcgg aggaacagcg taccgaactg
     1621 ctgcccctct cacaaaagcg ggccgaaacc gtacgagacg cgctcatcat gctcgggatc
     1681 gacagcgcgc gcatcaacac cagggcatac ggcggcgagc ggcccaaaac aaccgcgaac
     1741 gatacagagg aagcctggaa aaacagacgg gtggagttta ttctggaacg atagcgtcgg
     1801 gggtttgcgc agcctctgcg tctttccgtg ctcccgttgc ccgggcggcg acggaaaaaa
     1861 gggcggaaac aagcgctccc gaaatgatga gaagtccgcc ggtgtgcatg gactgcgagt
     1921 tcatgttgtc ggaaaaaccc cgggtcgtca gtacaattcc acccgcacaa agaaggcctc
     1981 cggcaaccat gagaacgcgg gatccatagt acataccgga tccggctttt tcgccggaga
     2041 tgtcatcaag aaccggccga ccatgcggcg gcgggggaac ccattgcgta tccggcgcgg
     2101 aataatttga aacgggaaat cgcacagaac gcacatcctc cccggccagg ggaagggcga
     2161 gtaacgcgaa aacacacgcg atgcttatgc atttggcaaa caagcttctc atcaacaaaa
     2221 agtatcacac ggtacggggg gcttgtccag actgctatta aaattgttga aaaatgccgc
     2281 gcgggagcac gtcttcttca gtatgtacgt tctttaatga acgtattgtt ccggatttcg
     2341 ctgaaagcag catcaagttc agcctgcgtg ttcatta
//
LOCUS       DAOOBX010000136         2365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_49660_length_2365_cov_3.293074, whole genome shotgun sequence.
ACCESSION   DAOOBX010000136 DAOOBX010000000
VERSION     DAOOBX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49660_length_2365_cov_3.293074"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /locus_tag="PLY30_02560"
     CDS             complement(<1..1705)
                     /locus_tag="PLY30_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPW65018.1"
                     /translation="MTVLLQINGICKAYGASVILENATAAFEDDQKIGVIGRNGAGKS
                     TLCRIITGHETADGGTVSKNAALRLSYLEQHDVFRDGESVLDYLMRATESEHWECGQM
                     AARFRIPNDMLEATVRSLPGGYQTRVKLAAMLLGDPNFLILDEPSNYLDLNTLILLEN
                     FLIDFRGGCLVVSHDREFLKRTCDHTLEVEGGELTLYPGDIEEYFEFKEVRTAQTEAH
                     NRGVEKKREQLQAFIDRFRAKASTASRAQSKMKQLAKLKTIEIGHPMSNVRIPIPPIE
                     AKAGIAFACEDLTVGYPEKDVARGIEFEIERGDRVAVLGDNGEGKTTLLRTMAGDLAP
                     KAGTFRWGTGLKTAFYAQHVFSALDPEDDIYTHLVRESHPGVTNQDVLNMAGCFLFKG
                     DDVKKKVKVLSGGERARLVLAGILLSKSHVLLLDEPTNHLDFETVESLGQALKRFNGT
                     VVFISHDRTFVNLVADKILEVKRGRVLRYPGSYEDYVYFREQVARGEREETVAGEMPE
                     PKKKGPVRDARAAPAGPAAKTPKPGAEIAKLRKALAKAEGRMKHFTEERDKILKEMAD
                     NP"
     gene            complement(2022..2365)
                     /locus_tag="PLY30_02565"
                     /pseudo
     CDS             complement(2022..2365)
                     /locus_tag="PLY30_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770078.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
BASE COUNT          454 a          719 c          664 g          528 t
ORIGIN      
        1 tgggattatc cgccatctct ttcaggattt tgtcgcgctc ctcggtgaaa tgtttcatcc
       61 ggccttccgc cttggccagg gccttcctga gtttcgcgat ctcggcgccc ggcttcggcg
      121 ttttcgccgc aggaccggcg ggggccgctc tcgcatcacg gaccggacct ttctttttcg
      181 gctccggcat ctcccccgca accgtttctt cccgttcccc ccgcgctacc tgttcgcgga
      241 agtaaacata atcttcatag ctccccggat acctcaggac ccgcccgcgt tttacttcaa
      301 ggatcttgtc cgcgaccagg ttcacgaagg tcctgtcgtg gctgatgaac acgaccgtac
      361 cgttaaaacg tttcagggcc tggccgagcg attccacggt ctcgaagtcc agatggttcg
      421 tcggttcgtc gaggagcagg acgtggcttt ttgagaggag gattcccgcc agcacaagcc
      481 tcgcccgttc gcctccgctc aggaccttca ccttcttttt gacgtcatct cccttgaaga
      541 ggaaacatcc cgccatattg aggacatcct ggttcgtaac gccgggatgg ctctcccgca
      601 caagatgcgt ataaatatca tcctccggat cgagggccga gaacacgtgc tgggcataaa
      661 aagccgtctt cagccccgtt ccccagcgga atgtccccgc tttgggggcg agatcacccg
      721 ccatggtcct cagcagggtc gttttcccct cgccgttgtc accgagcacg gcgacgcgat
      781 ccccgcgctc gatctcgaat tcgatcccgc gcgcgacatc cttttccgga tatcccacag
      841 tgagatcttc gcacgcgaac gcgatccccg ctttcgcttc gataggcggg atcggaatcc
      901 tgacgttcga catcggatgg ccgatctcta tcgttttcag tttcgcgagc tgcttcattt
      961 tcgactgggc gcgtgaagcc gtcgacgcct tggcgcggaa ccggtcgata aaggcctgaa
     1021 gctgctcgcg ttttttttca acgccccggt tgtgggcctc ggtctgggcg gttcggactt
     1081 ccttaaactc gaaatattcc tcgatgtccc ccggatacaa cgtcaattcg ccaccttcaa
     1141 cctccagcgt gtgatcgcag gtccgtttca gaaactcgcg gtcgtgggaa acgaccaggc
     1201 atccgcctcg gaaatcgatc aggaaattct cgagcaggat cagcgtgttg aggtccaggt
     1261 aattgctcgg ctcgtcaagg atcagaaaat tgggatctcc aagaagcatc gcggccagct
     1321 tcacgcgcgt ctgataaccg cccggaagac tgcggaccgt cgcctccagc atgtcattcg
     1381 gaatcctgaa acgggcggcc atctggccgc attcccagtg ttcgctttcc gtcgcgcgca
     1441 tcaggtagtc gagaacgctt tcaccatcgc ggaaaacatc gtgctgttcc agatagctga
     1501 ggcgtaacgc cgcgttcttc gagaccgtcc cgccgtcagc ggtctcgtgg cccgtgatga
     1561 tccggcaaag cgtggacttc ccggcgccat tccggccgat cacgccgatt ttctggtcgt
     1621 cctcaaaagc ggccgtggcg ttctcaagga tgaccgaagc gccgtaggcc ttgcaaatgc
     1681 cgttgatctg taaaaggacc gtcatgatcg ggattcgggg tttcggaacc ggatctcggg
     1741 aaagatcacc gggatcagcc gtgaaagata caaagacacg ccgtcaggcg gtcccgatcg
     1801 cataaaacgc gaccgtgttc gaaataaaac aaggtcaggc catcaggata acgctgttgg
     1861 ccagcgcctt gagaccggac tgccttttcc cgtgaacgaa agccgcaaat ccggtgcggg
     1921 gaaaaagaat ccggacgaaa agaccggacg cgttcattcc cttcctgtga aggacccgtc
     1981 ccggggatgt gttcagcgcg tcccccggag cgggaccggt atcaatactt gtaatgctcg
     2041 ggcttgtaag gaccctcgga aggcacgccg aggtaatcgg cctgcttcgg ggtaagcctg
     2101 gtgagcctgg cgccgagttt ctcgagatgg agccgggcga cttcttcgtc gagtttcttg
     2161 tcgagccggt aacacccggt ttcctggggt ttagcgcgag atcaatctgc gcgagcgtct
     2221 ggttcgtgaa ggaattcgac atcacgaaac tgggatgccc cgtcgcgcag ccgaggttca
     2281 cgaggcgccc ttccgcgaga aggataatcg agtgcccgtc gggaaacacg tatttgtcaa
     2341 cctgcggctt gatgttgatc ttctt
//
LOCUS       DAOOBX010000137         2360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_49804_length_2360_cov_2.619089, whole genome shotgun sequence.
ACCESSION   DAOOBX010000137 DAOOBX010000000
VERSION     DAOOBX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49804_length_2360_cov_2.619089"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLY30_02570"
     CDS             <1..346
                     /locus_tag="PLY30_02570"
                     /inference="COORDINATES: protein motif:HMM:NF021941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPW65019.1"
                     /translation="EVLRQFEREVKALARLETLECVREFKKQGAMVASSFADFDVCIS
                     VEGILDLAEEKKRLRKKVAETEGWIEGIRKKLDNPSFASRAPAEVLAKEKEKLSDAKK
                     ILLSYQEQLAGL"
     gene            297..1193
                     /gene="nadC"
                     /locus_tag="PLY30_02575"
     CDS             297..1193
                     /gene="nadC"
                     /locus_tag="PLY30_02575"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPW65020.1"
                     /translation="MPKKFSYPTRNNSPDSKAIRGTRGHSLPVSGRTIGFLREALRED
                     IGMKDVTSNLLIPAQARGTARVIAKEKGVFCGQWLVMKLFRIADPGVKLEFRVRDGHQ
                     FRRGQTVFLMHGNIRSILKVERTMVNFIGHLSGVATKTRLFVNKVKKYPVLILDTRKT
                     TPIWRELEKVAVQVGGARNHRQGLDEFIFVKENHRIHGTLKKLRRHPGNFEIEVRSLG
                     ELWEAIQLRPKVILLDNFTPNDAKRAVAIVRKEAPGVILEASGGITIGNVRKFAAAGV
                     DTISVGSLTHSVKAVDFSLLVD"
     gene            1225..1668
                     /locus_tag="PLY30_02580"
     CDS             1225..1668
                     /locus_tag="PLY30_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPW65021.1"
                     /translation="MPFSGRHAFRNISVIVLLAVVVAVWNYAEDLRTRFPDLDLSRSR
                     VGIASWYSRTDKGINERTANNEIFDDRAMTCASWDYPFGEKLLVINAMNGKWVVCRVN
                     DRGPALRLRRKIDLTKAAFKKIANPRRGLIYVSVIPVAKQREKRA"
     gene            1692..>2360
                     /locus_tag="PLY30_02585"
     CDS             1692..>2360
                     /locus_tag="PLY30_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPW65022.1"
                     /translation="METFRLVSGMTPKGDQPRAIEKLVEGFRSGKREQVLMGVTGSGK
                     TFTMAQVIARLGKPTLVISHNKTLAAQLYSELKEFFPENAVEYFVSYYDYYQPEAYIP
                     HTDTYIEKDASINDDLDRLRLSATSSLLSRKDCIVVSSVSCIYGLGAPEDWQGLLITV
                     KKGGPCERDKFLLGLIGLQYERVETELKRGTFRVRGGRIDLFPSYGETPYRIDLGAGT
                     VEAVR"
BASE COUNT          498 a          688 c          712 g          462 t
ORIGIN      
        1 cgaggttctg cggcagttcg aacgcgaggt caaggcgctc gcgcgtcttg agacactgga
       61 atgcgtccgc gagttcaaaa aacagggcgc gatggtcgcg agctcgttcg cggattttga
      121 cgtctgtatt tcggtcgaag ggattctcga tctcgcggaa gaaaaaaaac gtctccggaa
      181 gaaggtcgcc gaaacggaag gatggattga ggggatccgc aaaaagctgg acaatccctc
      241 attcgcctcc cgcgctccgg ccgaagtcct cgcaaaagaa aaggaaaaac tgagcgatgc
      301 caaaaaaatt ctcttatcct accaggaaca actcgccgga ctctaaggcc atccgcggca
      361 cccgcggtca ttcgctcccg gtctcgggac gcacgatcgg ttttttgcgg gaggctctca
      421 gggaagatat cgggatgaag gacgtgacct cgaacctgct tatcccggcg caggcgcgcg
      481 gtacggcgcg tgtgatcgcg aaagaaaaag gtgttttctg cgggcagtgg cttgtgatga
      541 agcttttcag gatcgcggat ccgggcgtaa agctggagtt ccgggtgcgc gacgggcacc
      601 agttccgccg ggggcagacg gtcttcctca tgcatgggaa cattcgttcc atcctgaagg
      661 tcgagcggac catggtgaac ttcatcgggc acctttcggg tgttgcgacc aagacgcgcc
      721 tgttcgtcaa caaggtgaag aagtatcccg tgttgatcct tgacacccgg aagaccactc
      781 cgatctggcg tgagctcgag aaggtcgccg tccaggtcgg gggagcccgg aaccaccggc
      841 agggcctgga tgaattcatt ttcgtcaagg aaaaccaccg gatccacggg accctcaaaa
      901 agctccgccg tcatcccggg aatttcgaga tcgaggtccg cagtctcggc gaactctggg
      961 aggcgatcca gttgagaccc aaggtgatcc tcctcgacaa tttcacgccg aacgatgcga
     1021 agcgcgctgt cgcgatcgtc cgtaaggaag cgccgggggt gatcctcgaa gcctcgggcg
     1081 ggatcacgat cggtaatgtt cgcaaatttg ccgccgccgg cgtcgacacc atttcggtcg
     1141 gatccctcac gcactcggtc aaggccgtcg atttctcgct cctcgtcgat tgatggacct
     1201 ctctacctga aacggagcga tcccatgccc ttctctggca gacacgcgtt cagaaatatc
     1261 tcggtgatcg ttctgctggc cgtcgtggtc gcggtctgga attacgcgga ggacctgcgg
     1321 acgcgtttcc ccgatctgga cctttcgcgt tcgcgcgtcg ggatcgcatc ctggtacagc
     1381 cggacggata aagggatcaa cgagcggacg gcaaacaacg agatattcga cgaccgggcg
     1441 atgacctgcg cctcgtggga ttatccgttc ggggagaagc tcctggtcat caatgccatg
     1501 aacggaaagt gggtggtctg tcgcgtcaat gaccgcggcc ctgccctgcg ccttcgccgc
     1561 aagatcgatc tcaccaaggc cgccttcaaa aagattgcga atcccagacg cgggctcatc
     1621 tatgtttcgg tcatccccgt ggcgaaacaa agggaaaaga gggcgtgacg ccggggtgct
     1681 ataatccctc catggaaacg ttcaggttag tgtccggtat gactccgaaa ggagaccagc
     1741 cccgggcgat cgaaaaactg gtcgaggggt tccgtagcgg caagcgcgag caggtcctga
     1801 tgggggtcac cggttcgggc aaaacgttca cgatggccca ggtgatcgcc cggctcggca
     1861 agccgacgct cgtgatctcg cacaacaaga ccctcgcggc ccagctttat tccgagctca
     1921 aggagttctt ccccgagaac gccgtcgaat acttcgtctc gtattacgat tattatcagc
     1981 ccgaggccta tatcccgcac accgatactt atatcgaaaa agacgcctca atcaatgacg
     2041 acctcgaccg gctccgtctg tccgccacga gctcgctcct ttcccgcaag gactgcatcg
     2101 tggtttcgag cgtctcctgc atctacggcc tcggcgcgcc cgaggactgg caggggcttc
     2161 tcatcacggt caaaaagggc gggccttgcg aacgggacaa gtttctgctc ggcctcatcg
     2221 gccttcagta cgagcgcgtc gagaccgaac tgaagcgggg gacgttccgt gtccggggcg
     2281 gaagaatcga cctctttccg tcctacggcg agacccctta ccggatcgat ctcggcgcgg
     2341 ggacggtcga agctgtccgg
//
LOCUS       DAOOBX010000138         2353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_49960_length_2353_cov_3.112707, whole genome shotgun sequence.
ACCESSION   DAOOBX010000138 DAOOBX010000000
VERSION     DAOOBX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49960_length_2353_cov_3.112707"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLY30_02590"
     CDS             complement(<1..432)
                     /locus_tag="PLY30_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HPW65023.1"
                     /translation="MNLSAPLRVGMIRYLNCLPFHFELEKLAKEKGLSVSFLESHPAD
                     LNRAMAKGEIDLAPFSSIEYLTHQDDYLLLPDLAIGAGNFVRSVILFSREPLASLNGA
                     TIALSEESFSSVILLRILLKRRFGLDNIFVTVAQDPLRALKD"
     gene            complement(509..1117)
                     /locus_tag="PLY30_02595"
     CDS             complement(509..1117)
                     /locus_tag="PLY30_02595"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006039262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HPW65024.1"
                     /translation="MKPYIVAVTGASGSLYAVRLLKFFEENRIPVELVVSDTARSVMK
                     EELGVQAPMFEKTGSDPDFRGMNIVLHSFHDFTASIASGSYPTSGMVVIPCSMGTLGA
                     IASGLSQNLIHRAADCTIKEGRRLVLVPRETPLSAIHLENMLKLARLGVRIVPPMPAF
                     YGAQQTVSDLVDFVVGKVLDQLEIPHALYPRWVGQIQTAETQ"
     gene            complement(1319..1705)
                     /locus_tag="PLY30_02600"
     CDS             complement(1319..1705)
                     /locus_tag="PLY30_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPW65025.1"
                     /translation="MAVLLLPVLVTFSMTGCASFGALPRGHSCVVLYGSEGCAACRTF
                     KRGLDREGVAYAFKDVEDGRVRKELFPRMERAGMDTGYFVLPVVDVNGQLACQPDLKD
                     TLALYRSSCTIRESVAGNQSIKNRPS"
     gene            complement(1732..>2353)
                     /locus_tag="PLY30_02605"
     CDS             complement(1732..>2353)
                     /locus_tag="PLY30_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPW65026.1"
                     /translation="SKGSVIAVSFLSLLLFEISAFILGPLCFLLSPVPFVLAAVYPVM
                     KRVSWISHFVLGSVLAIAPYGAWIASRGEFAPAPTFLSLGVICWVAGFDMIYALQDLD
                     FDREEGLHSFPARFGRELTLAATKWLHFLSVFFWALAGFFDGRGWGFFAGILLAAFFL
                     YRSYRLVRSYGVAKINEAFFVMNVVVSVGIFASAVLDLVFGRGSAG"
BASE COUNT          607 a          684 c          650 g          412 t
ORIGIN      
        1 gtccttgagc gcgcgtaacg ggtcctgggc gaccgtcacg aagatattgt cgagaccgaa
       61 acggcgtttg aggagaatgc ggagcaggat cacggagctg aagctttctt cagacagagc
      121 aattgtcgcc ccgttcaggg aagcgagcgg ctcccgggag aaaaggatga cgcttctcac
      181 gaaattcccg gcaccaatcg cgaggtccgg caggagaaga tagtcatcct gatgcgtcag
      241 gtactcgatc gaggagaacg gcgcgagatc gatctccccc ttcgccatgg cgcgattaag
      301 gtccgcggga tgcgactcga ggaagctgac cgaaagacct ttttctttcg cgagtttttc
      361 gagctcaaaa tgaaagggca ggcagttgag gtaacggatc atcccgacgc gaagcggagc
      421 ggagaggttc atgaagcggt ctcgctgtca catgccgcat gccacacgcc acacgggaaa
      481 aacctgttca tcctcttgtg accttgtgtc attgtgtttc tgcggtctgg atctgcccaa
      541 cccaccgggg ataaagcgca tgcgggatct cgagctggtc cagaaccttg ccgacaacga
      601 aatccacgag gtcggaaaca gtctgctgcg cgccgtaaaa agccggcatc ggcggcacga
      661 tccgtacgcc cagccttgca agtttcagca tgttttccag atggatcgcc gaaagcgggg
      721 tttcgcgcgg tacaagcaca agccttcttc cctctttgat cgtgcaatcc gccgcgcgat
      781 gaataaggtt ctgcgaaagc cccgatgcga tcgccccgag cgtgcccatg ctgcacggga
      841 tcacgaccat tccggacgtc ggatacgatc cgctcgcgat cgaggcggta aaatcatgaa
      901 agctgtgaag gacgatattc attcctcgaa aatcggggtc agaccccgtt ttttcaaaca
      961 tcggggcctg gacccccagc tcctctttca tcacactcct tgccgtatcc gagactacaa
     1021 gttcgacagg gatccggttc tcttcaaaga atttgagcaa acgcacggcg taaagagacc
     1081 cgctcgcacc cgtgaccgca acaatgtaag gtttcatatc cggtgtggga tattcaggtg
     1141 aaaggttcag ccgcgcgttc atcgggcatc gactccgttc cggcattcct gtccgggtag
     1201 aattgatcgg aaaaacatga ctggaaggat tgtattataa aagaaaccgg cgggtttgtc
     1261 atgggcagcc gccctttaaa accccggaaa tgagagactc gcagggctgg gaatccggtc
     1321 acgaaggccg gttttttatg gattgattgc cggcgacaga ttccctaatc gtacacgaag
     1381 accggtaaag cgcgagcgtg tccttcagat cgggctggca cgcgagctgc ccattcacgt
     1441 caacaaccgg caacacaaaa tatcccgtgt ccattccggc ccgttccata cgcgggaaca
     1501 attccttccg gacccttcca tcctcgacat ccttgaacgc atacgcgact ccctccctgt
     1561 ccaacccgcg tttaaaggtc cggcaggcgg cacatccctc gctcccatag aggacaacgc
     1621 aggagtgacc gcgcggcaaa gccccgaacg aggcgcagcc tgtcatcgag aacgtcacaa
     1681 gtaccggaag aagtaagact gccacgcccc cgacgcccct tcgggaaaac ctcatccggc
     1741 acttccccgg ccaaacacaa ggtcaagcac ggccgacgcg aaaattccga cgctgacaac
     1801 gacattcatg acaaagaacg cctcattgat cttcgcaacg ccgtaagagc ggacgaggcg
     1861 ataggaacga taaagaaaaa aagccgccaa aagaatgccg gcgaagaaac cccatccccg
     1921 gccgtcgaaa aatccggcaa gcgcccagaa gaacacgctc aaaaaatgca gccatttcgt
     1981 cgcggcgagg gtgagttccc gtccaaacct cgcggggaac gaatgaagtc cttcctccct
     2041 gtcgaaatcg aggtcctgca gggcgtagat catgtcaaat cccgcgaccc agcagatcac
     2101 gccgagcgaa agaaaggtcg gcgcgggagc aaactcaccc cggctcgcga tccacgcgcc
     2161 gtagggagcg atcgccagca cgctgcccag cacaaaatgc gaaatccagg aaacacgctt
     2221 catgacggga tagaccgccg cgagaacaaa gggaacggga gaaagtaaaa agcagagggg
     2281 gccgaggata aatgcggaga tctcgaacag cagaagcgaa aggaacgaaa ccgcgatcac
     2341 cgaacctttg gag
//
LOCUS       DAOOBX010000139         2342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_50297_length_2342_cov_2.941408, whole genome shotgun sequence.
ACCESSION   DAOOBX010000139 DAOOBX010000000
VERSION     DAOOBX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50297_length_2342_cov_2.941408"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PLY30_02610"
     CDS             complement(<1..1109)
                     /locus_tag="PLY30_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPW65027.1"
                     /translation="MVLRKSLGVLVLGSALLLSSAALAEDVQVTSSLDKRSVSVGEEI
                     HLTIRVTGPTANLQAPRLPAPDGFDSYYTGRASHMTFVNGVSSSSVEFSYTLIPQKAG
                     RFTIDPVQVQVGMQPYRTDPIVIEVLGDSGQVPSQSASRTSAPVVPQMPSQPQAYPQQ
                     TPQAPQPVVSQTPDDNIFVQAWIDRKSVYQNEQLLLTYSLYTRYDTRYEGFEKEPETS
                     GFWIEDFPMEREIERETVRMNGKRYVKADVKKTALFPTAPGTYSIQPGSLKVSIREEP
                     RGSSVFDEFFSDSFFNSGSFFARRQNRLLNPPPIQLTVKPLPESGKPAGFQGAVGQFR
                     ISATIDRDKVKQNEPVTMKLTIEGEGNIETLTRPK"
     gene            complement(1347..>2342)
                     /locus_tag="PLY30_02615"
     CDS             complement(1347..>2342)
                     /locus_tag="PLY30_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPW65028.1"
                     /translation="DRGPGARGIPSPNGCVRRVPVAEIAGDPAAGYRYRLEDALLRDS
                     IRRFGILMPLAVREGKRGFGVIAGHKRLAAAKTLRVREIPVMLIDRGMSDRDAFLACL
                     VSNWGQTTPDMDRARAVAKAIREFGMTRDEVTETVLPLLGLPADRNVFELYLKTDAVS
                     GPAKALFLEGKLSFRACPAFLRLDREDQDVFAREVAARLRFTASQGQQVLEWLADILK
                     RDGIDLRKLIRTKPFAGLLRRRGGDPRLAADRFHDAVRCCRFPSVSAFQAVFSGRADA
                     FTRDSKNLRIGPVQGFESEGFELHARLRSPEELERLIHKLAGNRSLLNSLFDFKL"
BASE COUNT          529 a          700 c          658 g          455 t
ORIGIN      
        1 atcttgggac gggtgagcgt ctcgatgttc ccctcacctt cgatcgtcaa cttcatcgtg
       61 acgggctcgt tctgtttcac cttgtccctg tcgatagtcg ccgagatccg gaactggccc
      121 accgcgccct ggaaccccgc gggtttcccc gattccggaa gaggcttcac ggtaagctgg
      181 atcggcggcg ggttcagaag ccggttctgc cggcgggcaa aaaaagaacc gctattaaaa
      241 aaactgtcag aaaagaactc gtcaaaaaca cttgaccccc gaggttcctc gcggatcgag
      301 accttgagac tcccgggctg gatcgagtag gtgcccgggg ccgtcggaaa aagggccgtc
      361 tttttcacat cggccttgac gtagcgtttg ccgttcatgc gtacggtctc acgctcgatc
      421 tcccgttcca tgggaaaatc ctcgatccag aacccgctcg tttccggttc tttttcgaat
      481 ccctcatacc gcgtgtcata acgggtataa agcgaatagg tcaggagaag ctgttcgttc
      541 tggtacacgg atttccggtc gatccacgcc tgaacaaaaa tattgtcatc gggcgtctga
      601 gacacaacag gctgaggcgc ctgcggagtt tgctgcggat aagcctgggg ctgggacggc
      661 atctggggaa ccaccggtgc cgaagtccgc gacgcggact gggaagggac ctgaccggag
      721 tcgccgagga cttcgatcac gatcgggtct gtgcgataag gttgcatacc gacctgcacc
      781 tgcaccggat cgatcgtgaa acggcctgcc ttctgcggaa tgagcgtgta gctgaactca
      841 acgctcgacg acgaaacacc gttcacaaaa gtcatgtggg acgcgcgacc cgtgtagtaa
      901 gaatcaaaac cgtcgggcgc cggaagacgc ggcgcctgca gattggcggt cggtccggtc
      961 acgcggatcg tcagatgaat ttcctctccc acgctgacgg aacgcttatc gagacttgag
     1021 gtcacctgca catcctcggc caatgcggcc gaagacaaaa gaagcgcgct tccgaggaca
     1081 aggacaccca aggattttct gagcaccata aaaccagaag acctttttgg agaactcgca
     1141 ctgtgtttga aatggctcac ggatttctat ccattgtttc cggcttgccg gccctcaaga
     1201 cccatcggga ttgacactgt catgaacgga taacctgctc cccggaagac gactctgaag
     1261 ccatggggcc acggctgatc gctatccgac gataacgcaa gcggttagaa tcaatctttc
     1321 aaaaaagccg tttgatttta tagtaactat aacttgaagt caaacaacga gttaagtaac
     1381 gagcgattcc cggccagttt gtgtatcaaa cgttcaagct cctccggcga ccggaggcgg
     1441 gcatggagtt caaatccctc gctttcaaat ccctgcaccg gcccgatgcg gagattcttc
     1501 gaatcgcgcg tgaacgcatc cgcccttccg gaaaaaaccg cctggaatgc cgagaccgag
     1561 ggaaaacggc aacaacgcac ggcatcatgg aagcggtcgg ccgcaaggcg cgggtccccg
     1621 cccctccggc gaaggagtcc cgcgaagggt tttgtcctga tcagcttccg aaggtcgata
     1681 ccgtcccgtt tgagaatatc cgcgagccat tccaggacct gctgcccctg ggaagctgtg
     1741 aacctgagcc gggcggcgac ttcgcgcgcg aaaacatcct gatcctccct gtcgagcctt
     1801 aaaaatgccg gacaagcccg gaaagaaagt tttccttcaa gaaaaagagc cttcgccgga
     1861 ccggacacgg catcggtttt gagataaagc tcgaacacat ttctatccgc cggcaagcca
     1921 aggagaggca acacggtttc ggtcacctca tcccgggtca taccgaattc gcggatggcc
     1981 ttcgcgaccg cccgcgcccg gtccatgtcc ggcgttgtct ggccccagtt ggaaacgagg
     2041 caggcaagga aagcgtcgcg atcgctcatc ccgcgatcaa tcagcatcac ggggatctcg
     2101 cggacccgga gggttttcgc cgccgcgagc cgtttgtgac ccgcgatcac cccaaatccc
     2161 cgtttccctt cacgaacggc aaggggcatc aggataccaa agcggcggat cgagtcacgc
     2221 agcaacgcat cctcaagccg gtagcgataa ccggctgcgg gatctcctgc gatctccgca
     2281 accgggaccc ggcgcacaca tccgttgggt gaaggaatgc cccgggctcc cggcccgcgg
     2341 tc
//
LOCUS       DAOOBX010000140         2336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_50485_length_2336_cov_3.265673, whole genome shotgun sequence.
ACCESSION   DAOOBX010000140 DAOOBX010000000
VERSION     DAOOBX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50485_length_2336_cov_3.265673"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLY30_02620"
     CDS             complement(<1..227)
                     /locus_tag="PLY30_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65029.1"
                     /translation="MIVTPNSRHNHKNDHPEEPAKASVPPVPETPKAAISPEEHEALR
                     KRVEELEGLREKFLHAAADFENAKKRALRDK"
     gene            complement(224..961)
                     /locus_tag="PLY30_02625"
     CDS             complement(224..961)
                     /locus_tag="PLY30_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65030.1"
                     /translation="MESHIETSDPVGSQFIVRRYSLRCSPATVRNELGVLEEMGYLSH
                     PHTSSGRIPTDDGYRYYLDHLPFDEELPETVSARVAEEISQGCRQERAIEAFLDRVSG
                     LLSSVTREIGLSLSMAPEPDLSRKIDELKLSLQGLTHILEKPEFRDIHKLKALLQTLE
                     EKILLKQWLLEKAHESKVSVSVGRENGSEALEDCAIVTARYSAGDLGTGVIAVLGPKR
                     MPYRRVIPLVSRVAGLIGELFASGEGF"
     gene            complement(1065..>2336)
                     /locus_tag="PLY30_02630"
     CDS             complement(1065..>2336)
                     /locus_tag="PLY30_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65031.1"
                     /translation="KEEIASIAEKEYGIRQCDVRINGTTLGVFLPLDQLFAMDFKEAI
                     LSGQVTDMEQLFQPTEKAIDRIEDMLFSMSRVMLSTDKKIDFYYLQAADTEKTGMEMM
                     FLGASDDVKKVRFWDIPRSEYRKRLIYEIQLNRPILWHRPVRKFFADLNTMTRQELKP
                     RYFKGQEDVKWMKEFYFEDATGYPLERAQASWEILDIRSLVLQDRQVVVYAKTRVTPK
                     GAKIPPETPEYLFHVSVDGEKEKITRIIPMAMMASAFDDPSFPMNRDMILKSLDRWDT
                     EFNVPDMTTGEFLALQLTRRIQMLIGQDERIYNTFSGVKVMLKYEPSKPCHYSLYMTA
                     PSKDIRQKVYSATGGINADVIYLWELAAREFADVLHGYGYKDWDYLMFFLSQTKSYSW
                     RASREDLELFRRKKKSLQDTLVLTTSRDTAA"
BASE COUNT          476 a          680 c          678 g          502 t
ORIGIN      
        1 tccttgtcgc ggagcgcgcg ttttttcgcg ttctcgaagt ccgccgcggc gtgcaggaac
       61 ttctcgcgga gcccttcgag ctcttcgaca cgttttctga gcgcctcgtg ctcttccggg
      121 gagatcgcgg ccttcggcgt ctccggcacc ggagggacgg aagccttcgc gggctcttca
      181 ggatggtcat tcttgtggtt atgcctggag ttcggagtca cgatcaaaac ccttccccgg
      241 atgcaaaaag ctcgccgatc agaccggcta cccgtgaaac aagcgggatc acccgccggt
      301 acggcatccg tttcgggccc aggaccgcga tcacgcccgt cccgaggtcc ccggccgaat
      361 accgggccgt cacgatcgca cagtcctcaa gggcttcgct gccgttctcg cgaccgaccg
      421 agaccgagac cttcgactcg tgggccttct cgagaagcca ctgcttcagg agaatcttct
      481 cctcgagtgt ctgcagcaac gccttcagct tgtggatgtc acgaaactcg ggtttttcga
      541 ggatatgcgt aagaccctgg agcgacagct tgagctcgtc gatcttccgg gaaagatccg
      601 gctcgggggc catcgaaagc gagagaccga tctcccgcgt gacggaggaa agaagtcccg
      661 aaaccctgtc gaggaacgcc tcgatggcgc gctcctggcg acaaccctgg ctgatctctt
      721 ccgcgacccg ggcagagaca gtctccggga gctcttcatc aaagggcaag tggtccagat
      781 aataacggta tccgtcatcg gtcgggatac gtcccgacga cgtgtgcgga tgcgacagat
      841 agcccatttc ctcaagcacg cccagttcgt tacgaacggt ggccggcgaa cagcggagtg
      901 agtagcggcg gacgatgaac tgcgagccca cggggtcgct cgtctcgata tgggactcca
      961 ccacactccg gagtaaaccg gattgtctcg gggttaacgg tatcataggg agggatatga
     1021 tagcagaaaa tgctccattc ctcaatacag tcccggggtc cggctcaggc ggcggtgtcg
     1081 cgggaggtag tcaggacgag ggtgtcctga agggattttt tcttcctgcg gaaaagctcc
     1141 aggtcttcgc gggaggcacg ccacgaatag gacttcgtct gggacaggaa gaacatcaga
     1201 taatcccagt ccttgtaacc gtaaccatga aggacatccg caaactcacg ggccgcgagc
     1261 tcccagagat aaataacatc tgcattgatg cctccggtcg cggaatagac cttctggcgg
     1321 atgtccttcg aaggggccgt catatagagc gaataatggc acggctttga aggttcgtac
     1381 ttcagcatca ccttgacacc ggagaacgtg ttataaatcc gctcgtcctg accgatcagc
     1441 atctggatgc gccgcgtaag ctgaagggca aggaattccc ccgtcgtcat atccggcacg
     1501 ttgaactccg tatcccaccg gtccaggctt ttcaggatca tatcccggtt catcgggaac
     1561 gacggatcgt caaacgcgct ggccatcatc gccatcggga tgatccgcgt gatcttttcc
     1621 ttttcgccgt cgacagacac gtggaaaagg tattcagggg tctccggagg aatcttcgcg
     1681 cctttcggcg tcaccctggt cttcgcataa acgacgacct gccggtcctg gagtacgagg
     1741 ctccggatat cgagaatctc ccaggacgcc tgcgcacgtt caagggggta tcccgtcgcg
     1801 tcctcgaaat agaactcttt catccacttg acgtcctcct ggcctttgaa ataccgcggc
     1861 ttgagctcct ggcgggtcat cgtgttcagg tccgcgaaga atttccggac cggccggtgc
     1921 cagaggatgg gacggttcag ctggatttcg taaatgaggc gtttgcggta ttcgctgcgc
     1981 ggaatgtccc agaagcggac cttcttcaca tcgtcgctcg cgcctaggaa catcatctcc
     2041 atccccgtct tttcggtgtc cgcggcctgg aggtaataaa aatcgatctt cttgtcggtg
     2101 gagagcatga cgcgcgacat cgaaaagagc atgtcctcaa tgcggtcgat cgctttttcg
     2161 gtcggctgga aaagctgttc catatcggtc acctggccgg agaggatggc ttccttgaaa
     2221 tccatcgcga agagctgatc gagggggaga aaaacgccga gcgtcgttcc gttgatcctc
     2281 acgtcacatt ggcggatgcc gtattccttc tcggcgatcg acgcgatctc ttcctt
//
LOCUS       DAOOBX010000141         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_50551_length_2334_cov_3.346204, whole genome shotgun sequence.
ACCESSION   DAOOBX010000141 DAOOBX010000000
VERSION     DAOOBX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50551_length_2334_cov_3.346204"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLY30_02635"
     CDS             complement(<1..301)
                     /locus_tag="PLY30_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65032.1"
                     /translation="MAQQEPFDAVEVTDTTGKLFTRTRSRRYMDWLRIGVYTLTIGVF
                     AALFTTGVLDRAENIVFDAFQRAGGFTRTDPRIALVEITGETIDEDRKTGITFGVS"
     gene            complement(303..1694)
                     /gene="mnmE"
                     /locus_tag="PLY30_02640"
     CDS             complement(303..1694)
                     /gene="mnmE"
                     /locus_tag="PLY30_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPW65033.1"
                     /translation="MPKLELEDTIAAIATPLGEGGISVIRLSGPGAFACARPFFTAPS
                     PTSIEEFPANTIHWGKIADREGRMVDQVLLSVFRAPHSYTGQDVLEVSCHGGLAVTKK
                     ILGLFLANGARHAEPGEFTRRAFLSGKIDLTQAEAVLDLIHAKSDRSLDIAVRQLAGQ
                     LSRRFKALKDLILKLLAHLEVYVDFPEEEPQTDVNEGMREKFSAIEAEMRSLLDGFGR
                     NQLVREGALVTILGKPNAGKSSLFNALLDRDRALVSDIPHTTRDHLEESLEMGGFYVR
                     IRDTAGLVPVPGHPLERLGMEKTLQAIAESHLVLFLVDGSVGLDDDDRHVFEKIPKEK
                     ALLVLVNKADLPMKLDAAGLAALTGGTDSIRISTWTREGLKELERKIAAFLEKETGEE
                     GEQITKLRHKDVLEKARDAVRRARETFDRKGSFELVSVDLRDAIEAMKELTGEIYSED
                     ILDVLFSEFCIGK"
     gene            complement(1695..>2334)
                     /locus_tag="PLY30_02645"
     CDS             complement(1695..>2334)
                     /locus_tag="PLY30_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPW65034.1"
                     /translation="RKATEKNHARVIEANPYRKGKAGLAGDFQNVNAGAAVQAARVLR
                     LVFGFRIDPATYGRALINRDWKGRMETVPNRGRVFILDGAHNPLSVRALARSFDTLCG
                     RGRQKRRLAVFGAMQDKDSKKMLEHLSRSFDSVVLTRVGNPRARSVGALLDEAKGLFK
                     CVVPAQNTEEALALARELTGDGEKILVTGSFYLAGEARKILKNGIRDQVLII"
BASE COUNT          474 a          710 c          651 g          499 t
ORIGIN      
        1 cggatacgcc gaaagtgatg cccgtcttgc ggtcctcatc gatcgtttcg ccggtgatct
       61 caaccagcgc tatccgggga tcggtccgcg tgaatccgcc ggcacgctgg aacgcatcga
      121 agactatatt ctccgcccgg tccagaacac ccgtcgtaaa gagcgccgca aagactccga
      181 tggtcagcgt atacacgccg atgcgaagcc aatccatata ccggcgggac cgggtgcggg
      241 tgaagagctt cccggtcgtg tcggtcactt cgactgcgtc gaacggttct tgttgggcca
      301 tattatttcc cgatgcaaaa ctctgaaaaa agaacgtcca ggatatcttc cgaataaatc
      361 tcgccggtca attccttcat ggcctcgatc gcgtcgcgaa gatcgaccga cacaagttcg
      421 aaggatcctt ttcggtcaaa ggtctctctt gcgcgacgca ccgcgtcacg ggctttttcc
      481 aggacatcct tgtgccggag cttggtgatc tgctcaccct cttctccggt ctccttttca
      541 aggaaagccg cgatcttccg ttcgagctct ttgagccctt cgcgggtcca agtggagatc
      601 cggatgctgt ccgttcctcc ggtcaaagcg gcaagtcccg ccgcatccag tttcatcgga
      661 agatccgcct tgtttacaag cacaagaagc gctttctctt tcgggatttt ttcgaaaaca
      721 tgacggtcat cgtcatccag gcccacggaa ccatcaacga ggaaaagcac gagatgactt
      781 tccgcgatgg cctggagggt cttttccatg ccgaggcgct caagcggatg cccggggacc
      841 gggacaaggc cggcggtgtc acggatacgg acataaaaac ctcccatctc gagcgactcc
      901 tcgagatggt cacgggtcgt atgcgggata tcggagacga gggcgcggtc gcgatcgaga
      961 agcgcgttga acagcgagga tttcccggcg ttcggttttc cgaggatcgt caccagcgca
     1021 ccctcgcgga ccagctggtt ccgtccgaat ccatcaagga gcgatcgcat ttcggcctcg
     1081 atcgccgaga acttttcacg catcccctcg tttacatcgg tctgcggctc ttcttccggg
     1141 aaatcgacat aaacctccag atgcgcgaga agcttcagga tcaagtcttt cagggccttg
     1201 aacctccggg aaagctggcc cgcgagctgc cggaccgcga tatcgagcga acggtcgctt
     1261 ttcgcatgga tgaggtcgag gaccgcttcg gcctgcgtga gatcgatctt gcccgacaga
     1321 aacgcccggc gcgtgaactc ccccggttcg gcgtggcggg ccccgttggc gagaaaaagg
     1381 ccgaggatct ttttcgtgac ggcaagaccg ccatggcagc tgacttcgag cacatcctgt
     1441 cccgtatagg aatgcggtgc gcggaacacg ctgagaagga cctgatccac catgcggcct
     1501 tcccggtcgg cgatctttcc ccaatggatc gtgttcgcgg gaaactcttc gatcgacgtc
     1561 ggtgacggtg cggtgaaaaa gggacgggca cacgcgaagg ctccggggcc gctcaaccgg
     1621 ataacggaaa tgccgccttc cccgagagga gtcgctatcg ccgcgattgt atcttcaagt
     1681 tcgagtttgg gcatttaaat aatcaggacc tgatcccgaa tcccgttttt caggatttta
     1741 cgcgcttcac cggccagata aaacgatccg gtcaccagga tcttttcccc gtcaccggtc
     1801 aattcacggg ccagcgcaag ggcttcttcg gtattttgcg cgggaaccac acatttgaaa
     1861 agccctttcg cttcgtcaag aagcgccccg acggaacggg cgcgcgggtt tccgacgcgg
     1921 gtgagaacga cgctgtcgaa actccggctc aaatgctcca gcattttttt cgaatcctta
     1981 tcctgcatcg ctccaaaaac cgcaaggcgt cttttttgcc gcccgcggcc gcagagggtg
     2041 tcgaacgatc tcgccagagc ccggaccgaa agcggattat gtgccccgtc gaggataaag
     2101 acccttcccc gattcggaac ggtctccatc cgtcctttcc agtcccggtt tatcagcgcc
     2161 cgcccgtagg tcgccggatc gatcctgaac ccgaagacaa gccggagaac ccgcgcggcc
     2221 tggaccgcgg cccccgcatt gacgttctga aaatcgccgg ccagtccggc ttttcctttt
     2281 cgataaggat tcgcttcgat aacgcgggcg tgatttttct cagtcgcttt ccgg
//
LOCUS       DAOOBX010000142         2330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_50661_length_2330_cov_3.188571, whole genome shotgun sequence.
ACCESSION   DAOOBX010000142 DAOOBX010000000
VERSION     DAOOBX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50661_length_2330_cov_3.188571"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PLY30_02650"
     CDS             complement(<1..749)
                     /locus_tag="PLY30_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPW65035.1"
                     /translation="MFDRFTERARKVIILAKEEAKRFNHDYIGTEHILLGLIKEGESV
                     AAAVLQNLGLSLDTIRLEIEKLVQFGPSTVVSGDIPFTPKAKKVIELAMDEARRLGHN
                     YIGTEHLLLGLIKEGEGVASHVLTNVGLDLNKVRSEVIKLLGSASPGGDAGGAPGQPA
                     SGIGGGSKAKTKTPALDAFGRDLSQLAREGKLDPVVGRHDEIERVIQILARRTKNNPV
                     LLGEAGVGKTAIVEGLAQRIIAGDVPEILID"
     gene            complement(796..>2330)
                     /locus_tag="PLY30_02655"
     CDS             complement(796..>2330)
                     /locus_tag="PLY30_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HPW65036.1"
                     /translation="RVVLPNLVAAFDPDSLRVRGRGTAAVKILGAQVERAFLKETPVA
                     RVKELEAQIEGIEDQNKKLENEKNVLKEEKSYLDSIRLFAGQKLPEDLVTRMPTAQDL
                     ESTLVFLGTRLKENFSRGLAIDLEIRENVKKLEVLRRELAELSGYGQKVKYSVVVDIE
                     VAKAGRLDLEAAYKVGGASWESVYDARAQLDKARTELVTYAIVRQTTGEPWDDVDMTL
                     STAQVNAGGSMPEVNSWFIHPYQPPVPVNRGKMMYAAKAGSLEAKSPGDFDMMRASAA
                     PPQLAMEPEMMIAETAYTQAESKGISVVYKLPRRVTIKPDGADHKVPVTAQDLVSNFE
                     YSAYPKMVPVAYLKSRVTNAKDIQLLGGKVNVFFEGDFVGNSRIPNIAPGEEFDLYLG
                     ADDNVKVKRELLEKKSDNVLIGNIPSSTKKIAYKYKLTAENYKAQAGKLSLFEAIPVS
                     QDERIKVKVTQISQEPQDKDWKDRKGVWRWELQLEPKAKKEIFYTVTVEFPRDMTVEG
                     LE"
BASE COUNT          441 a          717 c          625 g          547 t
ORIGIN      
        1 ttgtcgatca gaatctccgg gacatcgccc gcgatgatcc tctgggcgag cccttcaacg
       61 atcgcggtct tgccgacccc ggcctctccg agaagcaccg ggttgttctt tgtgcgccgg
      121 gcgagaatct ggatcacgcg ctcgatctcg tcatggcggc cgaccacggg gtcgagtttt
      181 ccctcacgcg cgagctgcga aagatcgcgt ccaaaagcgt cgagcgccgg cgttttggtc
      241 tttgccttgc tgccgccccc gatcccggaa gccggttgtc ccggggcacc gcccgcatcg
      301 ccgccgggcg aagccgatcc gagaagcttg atgacttctg aacggacctt attgaggtcg
      361 aggccgacgt tcgtgagcac gtgactcgca acgccttccc cctccttgat gagaccgagc
      421 agcagatgct cggtcccgat gtagttgtgc ccaagccggc gcgcctcgtc cattgcgagc
      481 tcgatgacct ttttcgcctt gggcgtgaac gggatatccc ctgacacaac ggtgcttggt
      541 ccaaactgca cgagcttctc gatctcgaga cggatcgtgt cgagcgaaag tccgaggttc
      601 tgcagtacag ccgcagcgac gctctccccc tctttgatca gaccgagaag gatgtgctcg
      661 gtcccaatgt aatcgtgatt gaagcgtttg gcctcttcct tcgcaaggat gatgaccttg
      721 cgggctcgct cggtgaaacg gtcgaacatg aaaaacctcc ggagatttta tggtacgatg
      781 acgaacgaca ctcgcttact cgagtccctc caccgtcatg tcccgcggga actcaacggt
      841 cacggtgtag aaaatctctt ttttggcttt cggttcaagc tgaagctccc agcgccagac
      901 acccttcctg tccttccagt ccttgtcctg gggttcctgg ctgatctgag tgaccttgac
      961 cttgatgcgc tcatcctggg agaccgggat cgcctcgaac aggctgagtt taccggcctg
     1021 ggccttataa ttttcggctg tcagcttgta tttataagcg attttcttgg tggacgacgg
     1081 aatattgccg atcaggacat tatcggattt cttctcgagc aattcccgct tcactttcac
     1141 gttgtcgtcc gcgcccagat aaaggtcaaa ttcctcgccc ggagcgatgt tcgggatacg
     1201 ggagttcccg acgaaatccc cttcaaagaa gacattcacc ttgcctccca aaagctggat
     1261 atctttcgcg ttcgtgaccc ggctcttgag atatgcgacc gggaccattt tcggatacgc
     1321 ggaatattcg aagttggaga ccaggtcctg ggcggtcacc ggcactttgt gatcggctcc
     1381 gtccggtttg atcgtaactc gccggggcag tttgtaaaca acggagatgc ccttgctttc
     1441 ggcctgggta taagccgttt cggcgatcat catttcgggt tccatcgcga gctgtggcgg
     1501 ggccgccgaa gctctcatca tgtcaaagtc acccggcgac tttgcctcca gggacccagc
     1561 ctttgccgca tacatcattt ttccgcgatt cacaggcacc ggcggctgat acggatgaat
     1621 gaaccagctg ttcacctcag gcatgctgcc gccggcattc acctgcgccg tcgaaagtgt
     1681 catatccaca tcatcccagg gttcgcccgt ggtctggcgg acaatcgcat aggtcacaag
     1741 ctcggtccta gccttgtcaa gctgggcgcg ggcatcatag acggattccc acgatgcccc
     1801 gccgaccttg taggcggcct cgagatcgag tcgaccggct ttcgccactt cgatatccac
     1861 cacgacggag tatttcacct tctgcccgta accggagagc tcggcgagtt cgcggcgcag
     1921 gacctcaagc ttcttcacgt tctcacggat ctcaaggtcg atcgccagac cgcggctgaa
     1981 attctccttc aacctggtac ccaggaatac aagcgttgat tccagatcct gggcggtcgg
     2041 catcctggtc accaggtcct cgggaagttt ctgccccgcg aagagcctga tcgaatccag
     2101 gtaagatttc tcttccttca aaacattctt ctcgttctca agttttttat tctggtcctc
     2161 gatcccttcg atctgcgctt caagctcctt cacccttgcg acaggggttt ccttgagaaa
     2221 agccctctca acctgggcac ccaggatttt cacggcggcg gtcccgcggc ccctcacgcg
     2281 aagcgagtcc ggatcgaaag cggccacaag attcggcaac acgaccctgg
//
LOCUS       DAOOBX010000143         2329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_50681_length_2329_cov_3.708883, whole genome shotgun sequence.
ACCESSION   DAOOBX010000143 DAOOBX010000000
VERSION     DAOOBX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50681_length_2329_cov_3.708883"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLY30_02660"
     CDS             <1..836
                     /locus_tag="PLY30_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPW65037.1"
                     /translation="PGPYEMDRARGEVVEQVIRPTGLMDPEIEVRRTGGQIDDLIGEI
                     RSRAKKGERTLITTLTKKMAEDLSRYLKELGIRGNYIHSEFDAFERVEILRDLRLKKY
                     DCVVGVNLLREGLDLPEVSLVIVLDADKEGFLRSDVSLIQIAGRAARHVNGKVIMYAD
                     KVTDSMKRAIDETARRRKIQEDYNKANGITPKSVIKEIREGIEKWKKAEEFVHNVVHE
                     SSAEHEAKNYLAFLKQRMESAARALEFDKAARYRDEIRKLESEKGVKKSVLGISPKKR
                     R"
     gene            complement(883..1914)
                     /gene="murB"
                     /locus_tag="PLY30_02665"
     CDS             complement(883..1914)
                     /gene="murB"
                     /locus_tag="PLY30_02665"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPW65038.1"
                     /translation="MKIEEHYPLKHLNTFGIDATARYFAEVRSADDLLELVRRAEFRQ
                     AKKLILGGGSNILFTQPFDGLVIRIAIPGIELTAHGAGVAKVRAGAGVNWQDLVDWTL
                     DRNLAGLENLSLIPGTAGAAPVQNIGAYGAEFKDVFEELQAFDLETGDMRTFLLKECG
                     FGYRTSVFKNEFRGRHVILSVSLRLLDLSTNGTAFRPVTVYGELGKRIEGIPSGELTP
                     RSVSEAVIAIRRAKLPDPKRLGNAGSFFKNPVIPAAQAQALLSAYPGMPRFTEPNGVK
                     IPAGWLIEQCGWKGKRVGNAGCHEHQALVLVNYGPATAKEILGLAYNIQRSVREKFGI
                     DLMPEVNIV"
     gene            complement(1946..>2329)
                     /locus_tag="PLY30_02670"
     CDS             complement(1946..>2329)
                     /locus_tag="PLY30_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65039.1"
                     /translation="AARARALMPRIEVAATGCAPYARAYAEAFQDWPAWMENGITDFV
                     IYMNYSSDPGEYTRFVAEAKPMVRDLSKWPIGVPAYKLTGSPETFARLYETCAATGES
                     ACVVFHYGSLVENPALEGPLLADTR"
BASE COUNT          480 a          674 c          755 g          420 t
ORIGIN      
        1 cgccgggacc ttacgagatg gaccgggcgc ggggggaggt cgtggaacag gtcatccgtc
       61 cgacgggcct gatggatcca gagatcgagg tccgccggac cgggggccag atcgatgacc
      121 tgatcgggga gatcagatcg cgcgcgaaaa agggagaacg gacgctcatc acgacgctca
      181 cgaaaaaaat ggcggaggac ctgagccgct acctgaagga gctcgggatc cgggggaact
      241 atatccattc cgaattcgac gcctttgagc gggtcgagat cctccgggac ctgcggttga
      301 aaaaatacga ctgcgtggtc ggtgtgaacc tgttgaggga ggggctcgat cttcccgagg
      361 tctcactcgt gatcgtgctg gatgcggaca aggaaggctt tctccggagc gatgtttccc
      421 tgatccagat cgcgggccgc gcggcgcgcc acgtgaacgg caaggtcatc atgtatgccg
      481 acaaggtgac ggattccatg aaacgggcga tcgacgagac cgcgcgacgg cgcaagatcc
      541 aggaagatta caacaaggcg aacgggatca caccgaagag cgtcatcaag gaaatccgcg
      601 aggggatcga gaagtggaag aaggccgagg aattcgtcca taacgtggtt cacgagtcat
      661 cggccgaaca tgaagcgaag aactaccttg ccttcctgaa acagcgcatg gaatcggccg
      721 cccgcgcgct cgagttcgac aaggccgccc gctaccggga cgaaatccgc aaactggagt
      781 ctgaaaaagg cgtcaaaaaa agcgtgctgg ggatttcccc gaagaagagg cgttagcccg
      841 gccatatccc gtccccggcc ggagaggccg tccccgccgc acctaaacga tattgacctc
      901 aggcatgagg tcgatcccga atttttccct cacggaccgc tggatgttat aggccagtcc
      961 gagaatctct ttcgcggtgg cgggtccgta attgacgagc acaagggcct gatgctcgtg
     1021 acagcccgcg tttccgacgc gcttgccctt ccagccgcat tgttcgatca gccagcccgc
     1081 cggaatcttg accccgttcg gttccgtgaa acgcggcatc ccgggatacg cgctgagaag
     1141 ggcctgtgcc tgcgcggcgg ggatgacggg gttcttgaaa aagcttccgg cgttcccgag
     1201 ccgtttcggg tccggaagtt tggcccggcg gatggcaatg accgcttcgc tgacggatcg
     1261 cggtgtcaac tcccccgacg ggatgccctc aatacgcttt ccgagctcgc cgtaaacggt
     1321 gaccggccgg aaggccgtgc catttgtcga caggtcgagc aggcgcaggg agacggaaag
     1381 gatcacgtga cggccccgga actcgttctt gaaaacactg gtccggtatc cgaagccgca
     1441 ttccttgagg aggaatgttc tcatatcgcc ggtctcgaga tcgaaggcct ggagttcttc
     1501 gaagacatcc ttgaactccg cgccgtaggc gccgatgttc tgcacggggg cggcccccgc
     1561 ggttccgggg atcagggaaa ggttctcaag gcccgcgagg ttccggtcga gcgtccagtc
     1621 caccagatcc tgccagttga cgccggcccc cgcgcgaacc ttcgcgacgc cggcgccgtg
     1681 agcggtcaat tcgatcccgg gaatggctat cctgatcacg aggccgtcga acggctgggt
     1741 aaaaaggatg ttgctgccgc cgcccaggat cagttttttt gcctgccgga attccgcacg
     1801 gcggaccagc tcgagaaggt catcggccga acggacttcc gcgaaataac gggcggtggc
     1861 gtcgatgccg aaagtattca gatgtttaag cgggtagtgt tcttcgatct tcatggcggg
     1921 attctacctc aacgggcggg attcgtcagc gggtatcggc aaggagcggt ccttcgagcg
     1981 ccgggttttc gacgaggctt ccgtaatgga acaccacgca ggcgctttct cccgtggccg
     2041 cgcaggtttc ataaagccgc gcgaaggtct ccggggaacc cgtgagcttg taagccggaa
     2101 cgccgatggg ccactttgag aggtcccgga ccatgggttt tgcttccgca acgaagcgcg
     2161 tgtactcgcc gggatcggac gaataattca tgtagatgac gaaatccgtg atgccgtttt
     2221 ccatccaggc cggccagtcc tggaaggcct cggcgtaagc gcgcgcgtac ggagcgcatc
     2281 cggtcgcggc gacctcgatg cgcggcataa gcgcccgggc ccttgccgc
//
LOCUS       DAOOBX010000144         2320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_50937_length_2320_cov_3.775717, whole genome shotgun sequence.
ACCESSION   DAOOBX010000144 DAOOBX010000000
VERSION     DAOOBX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50937_length_2320_cov_3.775717"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLY30_02675"
     CDS             <1..400
                     /locus_tag="PLY30_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPW65040.1"
                     /translation="ISHEVAHAIANHGGERMSQELVVQAGGMGISQLMKSRPEQARQI
                     MQQVYGAGAQYGVLLPYSRSHENEADHIGLILMARAGYDPKAAVAFWERMNKLGGAKV
                     PEILSTHPATSRRIANIEKEIPEAEKYYKS"
     gene            482..1939
                     /locus_tag="PLY30_02680"
     CDS             482..1939
                     /locus_tag="PLY30_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPW65041.1"
                     /translation="MSGICGVISKQDCAEKLFYGIDYHSHLGTEFGGIAMMDGERFSR
                     QIHEIAQSQFKSKFFEDLPGLKGNKGIGVISDSNEQPIYLNSRFGPFCIVTMGLIENA
                     DALVAELLAAGHSFAEMSSTGPVNTTELIARLITLGDDLADGIERMFERIEGSCSLLI
                     LNKDGIYAARDRRGYTPLVIGKHADTYAVTSETSAFPNTDLKVVKFMAPGEIILVTEN
                     GIASKREGNAEMQICSFLWIYTGFPASDYEGINTEAVRERCGAALARRDRDISVDVVA
                     GVPDSGTSHAIGYAMESGKPFRRPLVKYTPGYGRSYTPPIQQQRDLIAKMKLIPIREI
                     INGNRIAVLDDSIVRGTQLKNFTINKLWDAGAKEVHVRPASPPLMFPCKFCLATRSIH
                     ELAARKAIHNIEGRDVEDVSEYIDSGSPKHQKMVDSIAHDLGATTLRYQTVDDMVEAI
                     GLPREKLCLYCWTGECPSSGNKEKGNACRASCGKD"
     gene            2073..>2320
                     /locus_tag="PLY30_02685"
     CDS             2073..>2320
                     /locus_tag="PLY30_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65042.1"
                     /translation="MKKEIRFAFFVLGVTSVTAQVMILREFIASFYGNEFFLGWLLFA
                     WLFWTAAGSGLAGKRTRPPVLASGFVLMAAACFSGIIL"
BASE COUNT          490 a          637 c          719 g          474 t
ORIGIN      
        1 gataagccac gaggtcgctc acgcgatcgc caatcacggt ggcgaacgga tgagccagga
       61 gctcgtggtc caggcgggcg ggatggggat ctcccagctc atgaagtcca ggcccgaaca
      121 ggcgcggcag atcatgcagc aagtctacgg cgccggcgcg caatacggcg tgcttttgcc
      181 ttacagccgg tcgcatgaga atgaggctga tcatatcgga ttgatcctga tggcccgcgc
      241 cggttatgat cccaaggccg cagtggcgtt ctgggaacgg atgaacaagc tcggcggcgc
      301 aaaggtgccg gagattctct ccacgcatcc cgcgacttcc cgccgcatcg cgaacatcga
      361 aaaggaaatc ccggaagcgg aaaaatacta taaaagctga tccggatcgc ggcggcccgg
      421 ggcgggtctt gccctgtgcc gtccgcgctc tgtgcgtgtc attcgatcca ggggagattt
      481 tatgagcggt atttgcgggg ttatctcgaa acaggattgc gcggaaaaac ttttttacgg
      541 gatcgattat cattcgcatc tggggaccga gttcggcggc atcgcgatga tggatggcga
      601 acggttttca aggcagatac acgagatcgc ccaaagccag ttcaaatcga aattcttcga
      661 ggacctcccg ggcctgaaag ggaacaaggg gatcggggtg atcagcgact cgaatgaaca
      721 accgatctat ctcaattcca ggttcgggcc tttctgtatc gtgacgatgg ggctgatcga
      781 gaacgccgac gccctcgtgg cggaactgct cgcggccggg cactcgttcg ccgaaatgag
      841 ctcgacaggg ccggtcaaca cgaccgagct catcgccagg ctgatcacgc ttggggacga
      901 ccttgccgac ggcatcgaac ggatgtttga acggatcgaa ggttcctgtt cgctcctgat
      961 cctcaacaag gacggcattt acgcggcgag ggaccgccgg ggctacacgc ctcttgtgat
     1021 cgggaagcat gcggatacct atgcggtcac ctccgaaaca tcggcgtttc cgaacaccga
     1081 cctcaaggtc gtgaagttta tggcgcccgg agagattatc ctcgtgaccg agaacggtat
     1141 cgcgtcgaaa agggagggaa acgcggagat gcagatctgc tcattcctct ggatctacac
     1201 ggggttcccg gcgtcggatt acgaagggat caatacggag gccgtgcgtg aacggtgcgg
     1261 cgcggcgttg gcgaggcggg atagggacat cagtgtagac gtggtcgccg gcgtccctga
     1321 ttccggaacg agccacgcca tcggatacgc gatggaaagc ggcaagccgt tccgccgtcc
     1381 gctcgtgaaa tataccccgg gatacggacg cagttacacg ccgccgatcc aacagcagcg
     1441 ggaccttatc gcgaaaatga agttgattcc gatccgggag atcatcaacg gcaaccggat
     1501 cgcggtcctc gatgattcga ttgtgcgggg gacacagctc aagaatttta cgatcaacaa
     1561 gctctgggat gccggcgcga aagaggtcca cgtgaggccc gcgtctccgc ccctcatgtt
     1621 cccgtgcaag ttctgcctcg cgacccgcag tatccatgaa ctggccgccc gaaaggcgat
     1681 ccacaacatt gaaggacgtg atgtcgaaga tgtctcggaa tacatcgatt ccgggtcgcc
     1741 gaaacatcaa aaaatggtgg attcgatcgc ccatgacctc ggcgcgacga ctttgcgcta
     1801 ccagacggtt gatgacatgg tggaggcgat cggcctgccc agagaaaaac tgtgtcttta
     1861 ttgctggacg ggtgagtgtc cgtcgtcggg caacaaggaa aaaggaaacg catgccgcgc
     1921 ttcgtgcggc aaggattgat cgttcgcgct caaaaagatt cctccgggga ggccggtttt
     1981 ccggagccga tctcctctgt taaaatctcg cctttgggtg agtccaccgc ctgttaggtt
     2041 gtccctttct gtccgctgac gggagagatc ggatgaaaaa agagatacgt ttcgcgttct
     2101 tcgtgcttgg agtcacttcg gtgacggccc aggtcatgat tcttcgcgag ttcatcgcca
     2161 gtttttatgg gaacgagttt ttcctcgggt ggctcctttt cgcctggctc ttctggaccg
     2221 ccgccggcag tggcttggcg gggaaacgca cccgcccgcc ggtcctggcg tccggttttg
     2281 tgctcatggc cgcggcctgt ttttcgggga tcatcctgat
//
LOCUS       DAOOBX010000145         2300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_51578_length_2300_cov_3.563029, whole genome shotgun sequence.
ACCESSION   DAOOBX010000145 DAOOBX010000000
VERSION     DAOOBX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51578_length_2300_cov_3.563029"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..237
                     /gene="rpmI"
                     /locus_tag="PLY30_02690"
     CDS             37..237
                     /gene="rpmI"
                     /locus_tag="PLY30_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPW65043.1"
                     /translation="MPKMKTSKTMRKRFRVTKKGKVLANKTLRRHMMTDRSPKKKRQL
                     RRKLKAPKTKVSAIMMRLPYGT"
     gene            272..622
                     /gene="rplT"
                     /locus_tag="PLY30_02695"
     CDS             272..622
                     /gene="rplT"
                     /locus_tag="PLY30_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPW65044.1"
                     /translation="MPRTTHSVASRKRKKKIFKRAKGFVGGRRTLLRTAKETVARAMA
                     FATRDRKQRKRQFRQLWNVRINAACRENGLTYSRFINALRKANVTLNRKSLAEIAARD
                     PKSFKEILNFVKKA"
     gene            674..1645
                     /gene="pheS"
                     /locus_tag="PLY30_02700"
     CDS             674..1645
                     /gene="pheS"
                     /locus_tag="PLY30_02700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPW65045.1"
                     /translation="MIEIIRRIESEAQEALARAETGEALQSLRVAFLGKKGKFQDLFK
                     DFGAVPADQRKAIGAELNRVKDWLEQAIATAQARLENKGTDAQFDGTLPGVVPQTGSI
                     HVLQQTIDEITSIYGRMGFEVVEGPEIETEFHNFTALNIPLNHPSRDAFDTFYTTQGH
                     LLRSQTSTVQVRVMQSRKPPLRVVAPGRVYRPDTVDASHSFMFHQIEGLMVDDKVTFS
                     DLKGVLHLFLRELFGSAIKLRFRPHFFPFTEPSVEVDIQWGKGWLEVLGAGSVDPNVF
                     EAVGYDKSRVRGFAFGLGVERIAMLRHGITDIRHFYENDIRFLRQFA"
     gene            1642..>2300
                     /locus_tag="PLY30_02705"
     CDS             1642..>2300
                     /locus_tag="PLY30_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HPW65046.1"
                     /translation="MKVSTNWLRDFVTLAPPITRIADRLTLAGLEVKKVDQIDDLQDT
                     VFEIEITSNRPDWLSHVGVAREISAVEALSLKMPLLDIEAGRTPPAGWKIHIKDAEAC
                     PYYTGVLIEGITETRTPEFMVQRLLACGVRSVNLIVDITNYVLLEYGQPLHAFDADLI
                     QGREIQVRKAKPGERLLAINDATIELNPNDLVIADSDYAIALAGVMGGKKTEISDRTR
                     NV"
BASE COUNT          526 a          637 c          672 g          465 t
ORIGIN      
        1 cggccggacc aaccaagaca gagtgaggat ccagcaatgc ccaagatgaa gaccagcaag
       61 accatgagga aacgttttcg cgtcaccaag aaggggaagg tgctcgcgaa taaaaccctc
      121 cgccggcaca tgatgacgga ccggagtccc aaaaaaaaga ggcagctccg gcgcaagctc
      181 aaggccccga aaacgaaggt cagcgcgatt atgatgcgcc tgccttatgg aacttaaaca
      241 ccggtttgat cggtaagaaa ggaacactgt catgccaagg accacgcaca gcgtagcttc
      301 caggaagcgc aaaaagaaaa tcttcaagcg cgccaaggga tttgtcggcg gccgccgcac
      361 cctgttgcgc accgcaaaag agaccgtcgc gcgcgcgatg gcgttcgcga cccgcgatcg
      421 caaacaacgc aagcgtcagt tccgccagct ctggaatgtc cggatcaacg cggcgtgccg
      481 cgagaacggg ttgacatatt cgcgatttat caatgccttg agaaaggcca atgtaacgct
      541 gaaccgaaaa tcgcttgcgg agatcgcggc ccgcgatccg aagtcgttca aagagattct
      601 gaatttcgtc aaaaaagcct gatgtttttg agttcccggc tgttccctgc gttgctcagg
      661 cggagttcct ttcatgattg agataatccg gagaatcgag tcagaggcac aggaggcgct
      721 tgccagggcg gaaacgggtg aggccttgca atccctccgg gtcgctttcc tcggaaaaaa
      781 aggaaagttc caggaccttt tcaaggactt tggcgcggtt ccggccgatc agcgcaaggc
      841 gatcggtgcc gagctgaacc gtgtcaagga ttggctcgaa caggccattg cgacggccca
      901 ggccaggctg gaaaacaagg ggacggacgc ccagtttgac gggaccttgc ccggcgttgt
      961 tccgcagacg ggcagcattc atgtcctcca gcagaccatt gacgagatca cgtcgatcta
     1021 cggccgtatg gggttcgagg tcgtggaagg gcctgaaatc gaaaccgagt tccacaattt
     1081 cacggccctc aatattcccc tgaaccatcc gtcgcgcgac gcgtttgaca ccttttatac
     1141 gacccaaggg catctcctgc gttcgcagac gtcgaccgtt caggtgcgcg tgatgcaatc
     1201 gcgaaaaccg ccgctccggg tcgtcgctcc ggggcgtgtt taccggcccg acaccgttga
     1261 cgcgagccat tccttcatgt tccatcagat cgaaggcttg atggtcgatg acaaggtcac
     1321 cttcagtgat ctgaaaggcg tccttcacct ttttttgcgc gagctttttg gatccgcgat
     1381 caagcttcgc tttcgcccgc atttcttccc gttcaccgaa ccgagcgttg aggtggatat
     1441 ccagtgggga aaaggctggc tggaggttct gggggcgggg agtgtggacc cgaacgtttt
     1501 tgaggcggtc ggttacgaca agtcccgagt cagggggttc gcgttcggtc tcggggtcga
     1561 acgcatcgcc atgctccgcc acggcatcac ggacatacgg catttttatg aaaacgacat
     1621 ccgttttctg aggcaattcg catgaaggtc tcgacgaact ggcttcgtga ttttgtgacc
     1681 ctcgcgccgc cgatcacgcg catcgcggac cggctgacgc tggccggact tgaagtgaaa
     1741 aaggtcgatc agatcgatga tctccaggat acggtcttcg agatcgagat cacctcgaac
     1801 cgtccggact ggctttccca tgtcggggtt gcccgcgaga tttcggcggt cgaagcgctg
     1861 tcgctcaaaa tgccgctgct cgatatcgag gccgggcgaa ctcctccggc gggttggaag
     1921 attcatatca aggatgctga agcctgtcct tattacacgg gtgttctgat cgaggggatc
     1981 accgaaacca ggacgccgga attcatggtt caacgccttc ttgcctgcgg tgtccgcagt
     2041 gtcaacttga tcgtggatat cacaaactat gtcctgctcg aatacggcca gcctcttcat
     2101 gcctttgacg cagacctcat tcagggaaga gagatccagg tccgcaaggc gaagccgggg
     2161 gagagactcc ttgcgatcaa cgacgccacg attgagctga acccgaatga ccttgtaatt
     2221 gccgacagtg attacgcgat cgcgctcgcg ggtgtcatgg gaggaaaaaa gaccgagatc
     2281 tcggaccgca cgcgcaatgt
//
LOCUS       DAOOBX010000146         2279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_52201_length_2279_cov_3.508993, whole genome shotgun sequence.
ACCESSION   DAOOBX010000146 DAOOBX010000000
VERSION     DAOOBX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52201_length_2279_cov_3.508993"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PLY30_02710"
     CDS             complement(<1..172)
                     /locus_tag="PLY30_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HPW65047.1"
                     /translation="MRHRRLRRKLNVVSAHRKALLRNLVRALVIKKRIETTHAKAKEA
                     SAFADKLVTIAKR"
     gene            complement(194..1213)
                     /locus_tag="PLY30_02715"
     CDS             complement(194..1213)
                     /locus_tag="PLY30_02715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPW65048.1"
                     /translation="MGIRWKTFEMPKRLEADKNTLTATYAKFTAEPFERGYGTTIGNA
                     LRRVLISSIEGAAVTNIKIDGMLHEFATLDGVVEDGAQIVLNVKNLVLRYHGKGPKKI
                     YIDVKKEGPVKGSDIQADETVEIVNPDTVICTLSKAVQFKMEMEIGRGRGYVPAEKNK
                     QEVATIGVVNVDSIFSPVTKVNFTVEDTRVGQTTDYDRLILEVWTNGAMGPEEALLYA
                     SNILQRHLDIFVNYGELPEEEEEEEETDHGFMEMVNKPISELELSVRSANCLEAASIK
                     TIGDLVQKSEAQMLKYKNFGKKSLNEINQILASMGLQLGMNLDEKLGKKPAAAEADAS
                     APAAS"
     gene            complement(1253..1876)
                     /gene="rpsD"
                     /locus_tag="PLY30_02720"
     CDS             complement(1253..1876)
                     /gene="rpsD"
                     /locus_tag="PLY30_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPW65049.1"
                     /translation="MGRYRGPVIKLHRREGVNLGLKGRRATDEKHNKRLTNPPGQHGA
                     QRKKLSDYGVQMREKQKMKRIYGLGERQFRVVFHRASQKKGVTGENLIQSLERRLDNV
                     VYRLLFSTSRREARQMVGHGLITVNGRKVDIPSYEVKAGDKIAACAKEATRKRVQASL
                     EKWKDLSVPEWLSLDRTKGVGEVLRLPTKADTSLPVEESLIVELYSK"
     gene            complement(1880..2251)
                     /gene="rpsK"
                     /locus_tag="PLY30_02725"
     CDS             complement(1880..2251)
                     /gene="rpsK"
                     /locus_tag="PLY30_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPW65050.1"
                     /translation="MAKKKKIKHLARGVVRIAASFNNTIITITDTTGNALVWESSGSA
                     GFKGSKKSTPFAAQIASEKAAKKAMEYGMKEVEVYIKGPGAGRESAIRALQAAGLHVT
                     AIHDITPVPHNGCRPPKKRRV"
BASE COUNT          387 a          690 c          630 g          572 t
ORIGIN      
        1 cccgcttggc gatggtgaca agcttgtccg cgaacgcgct tgcttccttg gccttcgcgt
       61 gggtcgtctc gatccgtttt ttgatcacaa gagccctcac gaggttacgg agcaaagcct
      121 tgcggtgggc gcttacaacg tttaatttcc tgcgtagcct gcggtgacgc ataaattgcc
      181 tctaccttcc gtcttaagac gccgccgggg ccgacgcatc ggcctcagcc gccgccggtt
      241 ttttcccgag cttttcgtct aggttcatgc cgagctgaag gcccattgac gcgaggatct
      301 gattgatctc gttgagggac ttcttcccga aattcttgta cttgagcatc tgggcttcgc
      361 ttttctgcac aaggtcaccg atcgtcttga tgctggccgc ctcgaggcag ttcgcgctgc
      421 gaaccgacag ttccagttcg ctgatcggct tgttgaccat ttccatgaaa ccgtgatcgg
      481 tctcttcctc ttcctcttct tcttccggca gttcgccgta attgacgaag atgtcgaggt
      541 gacgctggag gatgttcgac gcgtagagga gggcctcttc cggtcccatc gccccgttcg
      601 tccagacctc gaggatcaga cggtcatagt cggtcgtctg gccgacgcgg gtgtcttcca
      661 ccgtaaaatt cactttcgtc accggtgaga agatcgaatc gacgttcaca acaccgatcg
      721 tcgcgacttc ctgcttgttc ttctcggcgg gcacataacc gcggccacgg ccgatctcca
      781 tctccatttt gaactgaacc gctttggaaa gcgtgcagat cacggtgtcc ggattgacga
      841 tctcaaccgt ttcgtcggcc tggatgtcgg acccctttac cggaccttcc ttcttgacgt
      901 cgatatagat cttcttcgga cccttgccgt gatagcggag cacgagattc ttgacgttca
      961 acacgatctg ggcgccgtcc tcgacaaccc cgtcgagcgt cgcgaactcg tgaagcattc
     1021 cgtcaatctt gatattcgtg accgcggcgc cttcgatcga ggagatcagc acccggcgaa
     1081 gggcattccc gatcgtcgtt ccatagccgc gctcaaaagg ctcggccgtg aatttggcgt
     1141 acgtggcggt cagggtgttc ttgtccgcct ccagacgctt cggcatttca aatgtcttcc
     1201 agcgtatacc catgatagtt tcctccttgt gcggaaaaag ataatttctg tgttatttcg
     1261 agtacaactc gacgatgagc gactcttcga ccggtagact cgtgtcggcc ttggtcggga
     1321 gcctcaggac ttcgccaaca cccttcgtcc tgtcgagact gagccattcc ggaacgctga
     1381 gatccttcca cttctcgagg ctcgcctgga cgcgcttccg ggtcgcttcc ttggcgcagg
     1441 cagcgatctt gtcacccgcc ttcacctcat aggaagggat atccaccttc cggccattca
     1501 ccgtaatcag accatgcccc accatttgac gcgcttcgcg gcgggaggtg ctgaacaaga
     1561 ggcgatagac gacattatca agacggcgct cgagcgactg gatgaggttt tcgcccgtga
     1621 cacccttctt ctgcgacgcc cggtggaaaa ccacacggaa ctggcgttcg ccgagcccgt
     1681 agattctctt catcttctgc ttttcacgca tctggacgcc gtagtccgaa agcttcttgc
     1741 gctgggcgcc gtgctgcccg ggagggttcg tgagccgctt gttgtgcttt tcgtccgtcg
     1801 cgcggcggcc tttgagaccg aggttgacgc cctcgcggcg gtgaagttta atgacgggac
     1861 cgcggtacct tcccatggat cagaccctcc tctttttcgg cggacggcag ccgttatgcg
     1921 gcaccggcgt gatatcgtgg atcgcggtca cgtgaagacc cgccgcctgc aacgcgcgga
     1981 tcgcggattc acggccggcg ccggggccct tgatgtagac ctcgacttcc ttcatgccgt
     2041 actccatcgc cttctttgcc gccttctcgg acgcgatttg cgccgcgaag ggggtggatt
     2101 tcttggaccc cttgaacccg gcggagccgg aggattccca gaccagggcg ttgcccgtgg
     2161 tgtccgtgat cgtgatgatc gtgttgttga aactggccgc gatgcggacg acgccacggg
     2221 ccaggtgctt gatcttcttt ttctttgcca taatcgttat accttttctt ttacaagtg
//
LOCUS       DAOOBX010000147         2266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_52593_length_2266_cov_3.254184, whole genome shotgun sequence.
ACCESSION   DAOOBX010000147 DAOOBX010000000
VERSION     DAOOBX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52593_length_2266_cov_3.254184"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /gene="guaA"
                     /locus_tag="PLY30_02730"
     CDS             <1..981
                     /gene="guaA"
                     /locus_tag="PLY30_02730"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPW65051.1"
                     /translation="RVCGCKKSWTPRSFINESVKKIRRQVGKDRVVLGLSGGVDSSVV
                     AGLIHKAIGKQLTCIFVNNGLLRRNEVERVNKTFRGHFKMNLKVIDAEKIFLARLKGV
                     TEPEQKRKIIGKTFIDVFQKEANRLGKVPYLAQGTLYPDVIESVSYFGGPTSTIKSHH
                     NVGGLPKNMKFKLIEPLKELFKDEVRAVGYALGIDKDIVGRQPFPGPGLAVRILGEVR
                     KDRCDLLRAADWIVVDEIKKSGYYDKVWQSFAVLLPVQSVGVMGDERTYENAVAIRAV
                     TSLDGMTADWAKLPYDLLGRISNRIINEVRGINRVTYDISSKPPATIEWE"
     gene            1061..>2266
                     /gene="dnaE"
                     /locus_tag="PLY30_02735"
     CDS             1061..>2266
                     /gene="dnaE"
                     /locus_tag="PLY30_02735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPW65052.1"
                     /translation="MTRSDFVHLHCHTEFSLLDSTCKIPGLMKRAAELRFPALAMTDN
                     GNLFAAIQFYSHAIKAGIKPIIGMRAYVAPGSRLEKQAHGIKEASFHLTLLARNEAGF
                     RNLTKLSSIGYTEGFYYRPRIDKEILKELGQDIIALSGGLKGEIPYYICHDQIDEAKR
                     VIDEYISIFGRENFFLEVMDHGLDPEKRVIAESCRLAKEFKIDLAATNDVHYIHQNEA
                     MAHDALICIGTGAKLEEPNRLRYHGDQYYLKSAEEMNHLFREIPDAIRNTVLIANRCN
                     IEFDFKKLYLPQFRPPEGKTQEQYLRELCQEQLKARMGGAIPEDYQKRLEMETTLIAK
                     MGYVSYFLIVWDFIRFSKDKGIPVGPGRGSAAGSLVSYALHITDIDPLKNHLYFERFL
                     NPERISMPDI"
BASE COUNT          504 a          661 c          655 g          446 t
ORIGIN      
        1 cgcgtttgcg gatgcaaaaa gagctggacg ccccgatctt tcatcaacga aagcgtgaaa
       61 aagatccgca gacaggtcgg gaaggaccgc gtcgtgctcg ggttgtcggg gggcgtggat
      121 tcctcggtcg tggcaggatt gatccacaag gccatcggaa aacagttgac ctgtatcttc
      181 gtcaataacg gactgttgcg ccggaacgag gtcgagcgtg tcaacaagac gttccgcggc
      241 catttcaaaa tgaacctgaa agtgatcgac gccgagaaaa tcttcctcgc gcgtttgaaa
      301 ggcgtgaccg agcccgaaca gaaacgcaag atcatcggca agaccttcat cgatgttttt
      361 cagaaggagg cgaacaggct cggcaaggtg ccgtacctcg cgcaggggac gctttatccc
      421 gacgtgatcg aatcggtctc ttatttcggc gggcccacga gtacgatcaa gagccatcac
      481 aatgtcggcg gtcttccgaa gaacatgaaa ttcaagctga tcgagcccct aaaggagctt
      541 ttcaaggacg aggtccgcgc ggtcggttac gcgctcggaa tcgacaagga catcgtcgga
      601 cgccagccgt tcccggggcc cggacttgcg gttcgtatcc tcggcgaggt ccggaaagac
      661 cgctgcgatc tcctgcgcgc ggcggattgg atcgtggtcg atgagatcaa aaagagcggt
      721 tactacgaca aggtgtggca gtccttcgcg gtcctgcttc cggtccagag cgttggcgtg
      781 atgggcgatg agcgcacgta tgagaacgcg gtcgcgatcc gcgcagtgac gtcgcttgac
      841 ggcatgacgg cggattgggc gaagctcccg tatgacctcc tcgggcgtat ctcgaaccgt
      901 attatcaatg aggtccgcgg catcaaccgc gtcacctacg acatctcctc gaaaccgccg
      961 gccacgatcg agtgggagta aaacgggttc catgccgcac gccgcgcgtc ccatgaaagt
     1021 ttcgtgcggc cttgtggcgt gtggccttat ggcgtgacct atgacacgtt ccgattttgt
     1081 ccatcttcat tgccacaccg agttcagtct tcttgactcg acgtgcaaaa ttcccggact
     1141 catgaagcgc gcggcggagc tccggttccc ggcgctcgcg atgacagata acgggaacct
     1201 cttcgccgcg atccagttct attcccacgc gatcaaggcc gggatcaaac cgatcatcgg
     1261 tatgcgtgcc tatgtcgcgc ccggttcccg cctcgagaaa caggcgcacg gtatcaagga
     1321 agcctccttc cacctgacgc ttctcgcccg gaatgaggcg gggttcagga acctcacgaa
     1381 actttcgagc atcggctaca ccgaggggtt ctactaccgt ccccgtatcg acaaggagat
     1441 tctgaaggag ctcggccagg acatcatcgc gctctcgggc gggctgaaag gcgagatccc
     1501 ttactacatc tgccacgacc agatcgacga ggcaaagcgg gtgatcgacg aatacatctc
     1561 gatcttcggt cgcgagaatt ttttccttga ggtcatggac cacggtcttg atcctgaaaa
     1621 acgcgtgatc gcggaatcct gccggctcgc gaaggagttc aaaatcgatc tcgcggccac
     1681 aaacgacgtt cactacatcc accagaacga agcgatggcg catgacgcgc tgatctgcat
     1741 cgggaccggt gcgaagctcg aggagccgaa ccgcctccgc tatcatgggg accagtatta
     1801 cctgaaatcg gccgaggaaa tgaaccatct tttccgggag attcccgacg cgatccggaa
     1861 cacggtcctc atcgcgaacc gctgcaacat cgaattcgat ttcaagaaac tctacctgcc
     1921 ccagttccgt ccgccggagg ggaagaccca ggaacagtat ctgcgcgaac tttgccagga
     1981 gcagctcaag gcgcgcatgg gcggcgcgat cccggaggac taccagaagc gtctcgagat
     2041 ggagacgacc ctgatcgcga agatgggcta cgtcagctac ttcctgattg tctgggactt
     2101 catacgtttt tcgaaagaca aagggatccc cgtcggtccg ggccgcgggt cggcggcggg
     2161 gagccttgtc tcctacgcgc ttcacatcac tgatatcgat ccgctcaaga accacctcta
     2221 cttcgaacgc ttcctgaacc ccgagcgcat cagcatgccc gatatt
//
LOCUS       DAOOBX010000148         2256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_52888_length_2256_cov_6.944116, whole genome shotgun sequence.
ACCESSION   DAOOBX010000148 DAOOBX010000000
VERSION     DAOOBX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52888_length_2256_cov_6.944116"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLY30_02740"
     CDS             <1..254
                     /locus_tag="PLY30_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65053.1"
                     /translation="VRGHQSDETKLISEFSVTFKKVRKASVAVSVGLSLAGRVSSMAS
                     AIARGGSTAGEDLPAGANISQLWSDPEDFKFSKTQAQYV"
     gene            251..592
                     /locus_tag="PLY30_02745"
     CDS             251..592
                     /locus_tag="PLY30_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65054.1"
                     /translation="MKAITALTADPKQNIKIVLDDGTTASLTLEFVASQRGWFYSITR
                     TGWTGVSRRRLVNSPNILRQFRGVIPFGFMCAVLDGQEPVYQDDFSTGRVQLYVLDQA
                     DLGTMETYIQS"
     gene            589..1458
                     /locus_tag="PLY30_02750"
     CDS             589..1458
                     /locus_tag="PLY30_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65055.1"
                     /translation="MSKFQRLFQLTFETRDGDKVVLTNPFTMQFNIVRHNLSSASNAN
                     IKIYNLGLANRKRIFFNAITTPEKFLHVELRAGYEGMMPIIFSGDILDAQSYRGEGSP
                     NFITEINAYDGGFDLAHADTNRGEAAGTLRVSAIRNLCKDLTHCKIGAIGNFPGAYAR
                     QRVFAGATKQLLEDETGGNFFIDNQRVYCLKDNEYVEGEVLVISSETGLLGSPTRCEM
                     IVTAEMLFEPRLKIGQVVELVSRDYPSLNQLYKVIGIQHSGTISDAVGGKCKTTVRML
                     YGIQNLTLVESES"
     gene            1455..2255
                     /locus_tag="PLY30_02755"
     CDS             1455..2255
                     /locus_tag="PLY30_02755"
                     /inference="COORDINATES: protein motif:HMM:NF037331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp138 family membrane-puncturing spike protein"
                     /protein_id="HPW65056.1"
                     /translation="MSEVIIPGQQEYPITKQDQVRFHTPDLAKLFAAKGQALKAEINC
                     VQVGTVQAFNAATQTVDVTLNVLRIVENVTKQYPVLVNVPVFIPSGGDGRLTMPITAG
                     DQCIVLFCDRSIEAWATAGTVAAPSSERTHDLSDGVAIVGFFPGSKPLSGLNQNGPEM
                     RHGTAKISIENNKIKIKNTAIDYGVQLASMWACRILMTASTLAQQKLMNTLLTALAND
                     SGLNSTSRAAASAAAGGGVIHEGVLTAQGANEVTASDNIGMIIDGSGV"
BASE COUNT          538 a          655 c          665 g          398 t
ORIGIN      
        1 cggttcgcgg gcaccagagc gacgaaacga aactgatctc ggagttttcc gtcacattca
       61 agaaggtgcg aaaagccagc gtcgcggtat cggtcggcct gtctttggcc gggcgcgtgt
      121 ccagcatggc ctcggcgatc gcacgcggag gatccaccgc cggagaggac cttcctgcag
      181 gcgcgaacat ttcccaactt tggagcgatc ctgaagattt taagttttct aaaacacagg
      241 cgcaatacgt atgaaagcga taacagcact caccgcggac ccgaaacaaa acatcaagat
      301 cgtcctggat gacgggacga cggcatcgct tacccttgaa ttcgtcgcga gccagcgcgg
      361 gtggttttat tcgatcacgc gcaccggatg gaccggggtg tcgcgccgcc ggctcgtgaa
      421 cagcccgaac attctccgac agttccgagg cgtgatcccg ttcgggttta tgtgcgcggt
      481 cctggacggc caggaacccg tttatcagga cgacttctcc acaggccgcg tccagcttta
      541 cgttctggac caggcagatc tcgggacgat ggagacctat atccagtcat gagcaaattt
      601 cagcgtttat tccagctcac gtttgagaca agggacggcg acaaggtcgt cctgacgaac
      661 ccgttcacga tgcagtttaa tatcgtccgg cacaacctct catcggccag caacgcaaac
      721 atcaagatct acaacctcgg cctcgcgaac cggaagcgca ttttttttaa cgcgatcacg
      781 accccggaaa agtttctcca cgttgagctc cgggccggat acgaaggcat gatgccgatc
      841 atttttagcg gagacattct ggacgcgcag tcttaccgag gcgaaggatc cccgaacttc
      901 atcacggaga taaatgccta cgacgggggg tttgacctcg cacacgcgga tacgaacagg
      961 ggcgaggcgg ccgggacgct tcgtgtgagc gcgatccgga acctttgcaa ggatctgacg
     1021 cattgcaaga tcggagcgat cgggaacttc ccaggagcct acgcccgcca gcgcgtgttc
     1081 gccggcgcga caaagcaact gctcgaggac gagaccggcg ggaacttctt catcgacaac
     1141 cagcgcgtct actgcctgaa ggacaacgag tacgtcgaag gagaggttct ggtgatcagc
     1201 tccgagacag gtctcctcgg aagcccgacc cggtgcgaga tgatcgtaac ggcggagatg
     1261 ctctttgagc cgcgcctcaa aataggccag gtcgtcgagc tcgtgagccg ggactacccg
     1321 agcctaaacc agctttataa agtgatcggg atccagcaca gcggaacgat ctccgacgcg
     1381 gtcggcggga aatgcaagac cacggtccgc atgctttacg ggatccagaa cctgaccctc
     1441 gtggagagcg aatcatgagc gaagtcatta taccgggaca gcaggagtac ccgatcacga
     1501 agcaggacca ggtccgattc cacaccccgg acctcgcgaa acttttcgcg gcgaaaggcc
     1561 aagctctcaa ggccgagatc aactgcgtcc aggtcgggac cgttcaggca ttcaacgccg
     1621 cgacgcaaac cgtggacgtg acgcttaacg tcctccgtat cgtggaaaac gtcacgaaac
     1681 aatacccggt ccttgtgaat gtccccgttt ttatcccaag cggaggtgac ggccgcctaa
     1741 cgatgccgat cacggccggg gatcaatgca tcgtcctttt ttgcgaccga tcgatcgagg
     1801 cgtgggccac cgccgggacc gtcgcggcac cgtcaagcga gcggacgcac gatctctcgg
     1861 acggcgtggc gatcgttggc ttctttccgg ggagcaaacc gctgtcaggc ctgaaccaaa
     1921 acggcccgga gatgaggcac ggaacggcca agatcagcat cgagaacaac aagatcaaga
     1981 tcaaaaacac ggcgatagac tatggagtcc agctcgcgtc catgtgggcc tgccggatcc
     2041 ttatgacagc atcgaccctg gcacagcaga aacttatgaa cacgcttctg acagccctgg
     2101 caaacgacag cggactgaac tcgacgagcc gggcggcggc ttcagcggcg gccggaggcg
     2161 gagtaataca cgaaggggtg ctgaccgccc agggggcaaa cgaagtgacg gcaagcgata
     2221 acatcggaat gatcatcgac gggagcggag tatgat
//
LOCUS       DAOOBX010000149         2233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_53595_length_2233_cov_3.516988, whole genome shotgun sequence.
ACCESSION   DAOOBX010000149 DAOOBX010000000
VERSION     DAOOBX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53595_length_2233_cov_3.516988"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLY30_02760"
     CDS             <1..198
                     /locus_tag="PLY30_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65057.1"
                     /translation="PRHGFVLRNTLNGYRMPSSACKILRAVDLSRENFSPFGSSLNFP
                     ACRETSSASLFSWERRTLGAA"
     gene            244..954
                     /locus_tag="PLY30_02765"
     CDS             244..954
                     /locus_tag="PLY30_02765"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPW65058.1"
                     /translation="MLKILRLHTKTIVWFVVVSFVIWGGYSMTTLRKEGRYAGEAFGK
                     PVTFQEYNKFYRATELFMPEKNRSELEDPEVVRTFTWQNIVYSREAQRLGIAVTDSEV
                     RDQIATILTQQGLSNPTREQYHIWLTRNLRVTPREFEEGLREIMRIQKFLKTKFEEFT
                     PKASGEKPADPKKAEEELAQKKQASFILWTNDLNQRAKLRDYLAVSTGEKPAPEAAVL
                     PDGAGETAQASPASVPAK"
     gene            967..1566
                     /locus_tag="PLY30_02770"
     CDS             967..1566
                     /locus_tag="PLY30_02770"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HPW65059.1"
                     /translation="MKKSVQEIARIVGGTVVGDASFIPEGITNIENPLPGHITFAQDE
                     KGFRALETSGIACLIVPKQITQSSKVLIQVEHPKRAWAKLLREIYPPRKFVPGISPEA
                     SVAASAKIGANVTVEAFAVIGENSTVSEGTVIRSGAFIDENVKIGANCVIHPNVTIYR
                     DSDLGANVIIHAGTAIGADGFGYVATPTGRKNFRRSGMS"
     gene            1563..2003
                     /locus_tag="PLY30_02775"
     CDS             1563..2003
                     /locus_tag="PLY30_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65060.1"
                     /translation="MIEDDVEIGACCTVDRAALGSTRIGKGVKIDNLVQIAHNVVIGP
                     HTVISAQTGISGSCKIGSHVTMGGSVGVGDHVEIGDWAMVGAGAGFPSNKKVPGKQIY
                     FGQPARPYAEMRKQFGAQLRSAETLDDVRALKKKVAELEKKLAS"
     gene            complement(2080..>2233)
                     /locus_tag="PLY30_02780"
     CDS             complement(2080..>2233)
                     /locus_tag="PLY30_02780"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HPW65061.1"
                     /translation="VIEALHLCMCMRGVKKSDSYTVTSSMLGAFRKDARSRSEFLSLI
                     RLKRED"
BASE COUNT          506 a          672 c          636 g          419 t
ORIGIN      
        1 ccccggcatg gatttgtgct ccgaaatacg ttgaacggat atcgtatgcc gtcttctgct
       61 tgtaaaatac taagggcggt ggatttgtcg agggaaaact tctcgccgtt tggctccagc
      121 ctcaatttcc cggcctgccg ggaaacgtca tcggcttctc tcttttcctg ggaacggcgc
      181 accctcggcg cggcgtgact ttgtttgtca tttgagggag cttatgctaa attatccgcc
      241 gttatgttaa aaattctccg gctccacacc aaaacgatcg tctggttcgt cgtcgtctcc
      301 ttcgtgatct ggggcggtta ctccatgacc accctgagaa aagaaggccg ttacgcgggt
      361 gaggccttcg ggaaacccgt cacctttcag gaatacaata agttttaccg cgccaccgaa
      421 ctttttatgc cggagaagaa ccgttccgaa ctggaagacc cggaagtggt ccggaccttc
      481 acctggcaga acattgttta ctcccgcgag gcccaacgcc tcggtatcgc ggttacagac
      541 agtgaggtcc gcgaccagat cgcgacaatc ctgacccagc agggactttc gaacccgacg
      601 cgggagcagt atcatatctg gctcacgcgg aacctcaggg taactccccg ggaattcgag
      661 gaaggtctgc gcgagatcat gcgcatccaa aagttcctga agaccaaatt cgaggaattc
      721 acgcccaagg catccggaga aaaacccgcg gaccccaaga aagcggaaga agaactcgcc
      781 cagaaaaaac aggcatcctt catcctctgg acgaacgact tgaaccagcg ggccaagctc
      841 cgggattatc tcgcggtatc aaccggtgaa aaacctgcgc ctgaagccgc agtcctcccg
      901 gacggcgccg gggaaactgc gcaggcttcc ccggcatccg ttcccgcaaa ataaggaaac
      961 gcccttatga aaaaatctgt tcaagagatc gcccggatcg tcggcggcac cgtcgttggc
     1021 gacgcctcct tcattcctga aggcatcacg aacatcgaga atcccctccc cggacacatc
     1081 acattcgcgc aggatgaaaa agggttcagg gcactcgaaa cctccgggat cgcctgtctg
     1141 atcgtcccca aacagatcac gcagtcgtcg aaagtgttga tccaggtcga gcacccgaaa
     1201 cgcgcgtggg ccaaactcct ccgggagatc taccctcccc gcaaattcgt ccccggcatt
     1261 tctccggagg cctcggtcgc cgcttccgcg aagatcggcg ccaatgtcac ggtcgaagcg
     1321 ttcgccgtca tcggggagaa cagcacggtc tcggagggaa cggtgatccg ctccggggcg
     1381 ttcatcgacg agaatgtgaa gatcggcgcg aactgcgtca tccaccccaa cgtgacgatc
     1441 taccgggact cggatctcgg cgcgaacgtg atcattcacg caggaacggc gatcggcgcg
     1501 gacggtttcg gctatgtcgc gaccccgacg ggcaggaaaa acttccgcag gtcgggaatg
     1561 tcgtgatcga ggatgatgtc gagatcggcg cctgctgcac cgtcgaccgg gcggccctcg
     1621 gttcgacccg gatcggcaag ggtgtgaaga tcgacaacct cgtccagatc gcccacaatg
     1681 tcgtgatcgg gccccacacg gtcatctccg cgcagaccgg catctccgga agctgcaaga
     1741 tcggatccca tgtcaccatg ggcggcagtg tcggggtcgg agaccacgtc gagatcggcg
     1801 attgggcgat ggtcggtgcc ggggcgggat tcccttccaa caagaaagtc cccggcaagc
     1861 agatctactt cggccagccc gcgagacctt acgccgagat gcggaagcag ttcggcgcgc
     1921 agctccgctc cgcggaaaca ctcgatgacg tccgcgcgct caagaagaaa gtcgcggaac
     1981 tcgaaaagaa gctcgcttct taaacacacg attcacgccg caggtcaccc gatccgacag
     2041 aacagggtgc gtgtgtcttt gcggccttgt ggccaggaac taatcttctc tcttcagacg
     2101 gatcagactg aggaattccg agcgggagcg ggcgtccttg cggaaggcac cgagcatgga
     2161 gctcgtgacg gtgtaggaat cggacttttt gacgccgcgc atacacatgc aaagatgcag
     2221 ggcctcaatc acg
//
LOCUS       DAOOBX010000150         2229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_53713_length_2229_cov_3.739650, whole genome shotgun sequence.
ACCESSION   DAOOBX010000150 DAOOBX010000000
VERSION     DAOOBX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53713_length_2229_cov_3.739650"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PLY30_02785"
     CDS             <1..831
                     /locus_tag="PLY30_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65062.1"
                     /translation="PKIPELSGFKTYDADTSSQLFKTGNVIGGAKSFEIAFIPKDEGK
                     VFIPPLDFSFFDPRAEKYVTLRTPNFPVTVEKSDQEFKMPEEISAKGIFKKQVKLEAK
                     DISFIAERLPDPLPAKVFHLAYRVLLGLNVLLLIVLGVLFFKSREEEIFSKDEGLKRR
                     KRARAVAQMNLKKLGRLARGTKPEQTAAFFVELEHSLTQYISDKLNLSTYGVTRRDLE
                     DALANLLGAEDPLLKELIQLYDLCDESGFGKGAIPQASKKIGMKIFQDTVNRLERVRS
                     "
     gene            828..1616
                     /locus_tag="PLY30_02790"
     CDS             828..1616
                     /locus_tag="PLY30_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW65063.1"
                     /translation="MTDSLTGKRMVAGKCLFLLALFLGTIVPAGLAVQPDKKFEEANA
                     SYRTGDYEHAAKLYRELSETDPGNSAYLYDLGNACVRLGRLSEAILAFEKALLIAPRD
                     KDIRQNRDYARSLLEYRVDDNRNWYLRAAESALSRFTQTEMNFFALSFAAIFLTAAIC
                     FYVFRQTVFWGAGRRTLLVIALLAAAVAFYKQARSGLVRPSIVMTKECEARYGPSAHD
                     QVAFRMGEGIRVFLVDRREDWSRVLLTNGESGWVRNGDIEEIGQ"
     gene            1613..>2229
                     /locus_tag="PLY30_02795"
     CDS             1613..>2229
                     /locus_tag="PLY30_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPW65064.1"
                     /translation="MKLPDVKGHFGPYGGRYVPETIIKACEELTAAYLKVRSEKKFRE
                     ELACYLADYAGRPTPVYEAKNLSKHYGRARIFLKREDLLHTGAHKINNTIAQAILAKR
                     LGRTRIVAETGAGQHGVASATAACVFGMKCVVYMGEVDIERQALNVYKMKLLGAEVRS
                     VKSGSRTLKDATNEAIRDWVTNVEDTHYMMGSVAGPHPYPMMVRD"
BASE COUNT          481 a          592 c          694 g          462 t
ORIGIN      
        1 cccaagattc ctgaactgag cgggttcaag acctacgacg cggatacaag ttcccagctg
       61 ttcaagaccg ggaacgtgat cggcggcgcg aagagttttg agatcgcatt tatcccgaag
      121 gacgagggca aggtgttcat cccgccgctt gatttcagct ttttcgatcc gcgcgccgaa
      181 aagtacgtga ctctccggac gccgaatttc ccggtgacgg tcgagaagtc ggaccaggag
      241 ttcaaaatgc cggaagagat ctccgcgaag gggatcttca agaagcaggt caagctcgag
      301 gccaaggaca tcagctttat tgccgaacgc cttcccgacc cgctgccggc aaaggtcttt
      361 cacctggcgt accgggtcct tctgggtctg aacgttcttc tcctgatcgt tctcggcgtt
      421 ctgttcttca agagtcgtga agaagagatc ttctcgaagg atgaagggtt gaagcgtcgc
      481 aagcgcgccc gagcggtcgc gcagatgaac ctgaaaaagc tcggccggct cgcgcgcggg
      541 acaaagcccg aacagaccgc ggcgttcttc gtggagctcg agcattcgct gacccagtat
      601 atttccgaca agctgaacct ttcaacctac ggcgtgaccc ggcgcgacct cgaggatgcg
      661 ctcgcgaatc ttctcggcgc cgaggacccg ctcctgaaag agctgatcca gctctacgat
      721 ctttgtgacg agtccggatt cggaaaaggg gcgatccccc aggcttccaa aaagatcggt
      781 atgaagatct ttcaggatac cgtgaaccgg ctggaaaggg tgcggtcatg acggattcct
      841 tgacgggaaa gaggatggtt gccgggaaat gcttgttcct cctggccttg tttctgggaa
      901 cgatcgtgcc ggcgggtctt gccgtacagc cggacaaaaa attcgaagag gcgaacgcga
      961 gctatcgtac cggagactat gaacacgccg caaaattgta ccgggaactc tccgagaccg
     1021 atccgggtaa cagcgcttat ctttatgacc tcgggaacgc gtgcgtcagg cttgggcgcc
     1081 tgagcgaggc tattctggct ttcgaaaagg cgttgctgat cgcgccgcgg gacaaggata
     1141 tccgccagaa ccgcgattac gcgcggagtc tgctggaata ccgcgtggac gataaccgca
     1201 actggtatct gcgcgcggcc gaatcggcgc tgtcgcgctt tacgcagacc gaaatgaatt
     1261 ttttcgcgct gtcctttgcg gccatttttc tgacggcagc gatctgtttc tatgtcttca
     1321 gacaaacggt gttttggggc gccggtcgac ggacgcttct tgtcatcgcg ttgcttgcgg
     1381 cagcggtggc gttttataaa caagcccggt ccgggctggt tcgtccttcg atcgtcatga
     1441 cgaaagaatg cgaagcccgc tacgggcctt cagcccacga ccaggtcgcg ttccgcatgg
     1501 gcgaggggat ccgggtgttc cttgtggacc gtcgcgagga ctggagccgt gtcctgctca
     1561 cgaacggtga gagtggctgg gtaaggaacg gggatatcga ggagatcgga caatgaaact
     1621 tcccgacgtg aaggggcact tcggccctta tggcggacgg tatgtgccgg aaaccattat
     1681 aaaagcgtgt gaggagttga cggccgctta tctgaaggtc cgatcggaga agaaattcag
     1741 ggaagagctc gcttgttatc ttgcggacta cgcgggccgg ccgacgcctg tttatgaagc
     1801 gaaaaacctg tcaaaacatt acggaagggc ccgaatcttt ttgaagcggg aggatttgct
     1861 ccacacggga gcccacaaga tcaacaatac gatcgcgcag gcgatcctcg cgaagcgtct
     1921 cggcaggacg cggatcgtcg ccgaaacagg ggcggggcag cacggtgtcg cgtccgccac
     1981 ggccgcttgc gtcttcggca tgaaatgcgt ggtctacatg ggtgaggtcg atatcgagcg
     2041 ccaggccctg aacgtctaca agatgaaact gcttggcgct gaagtccggt ccgtgaagtc
     2101 gggttcccgg acactcaagg atgcgacgaa cgaagcgatc cgcgattggg tcacgaatgt
     2161 ggaagatacc cattacatga tggggtcggt ggcggggccg catccgtatc cgatgatggt
     2221 ccgtgattt
//
LOCUS       DAOOBX010000151         2207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_54394_length_2207_cov_2.434480, whole genome shotgun sequence.
ACCESSION   DAOOBX010000151 DAOOBX010000000
VERSION     DAOOBX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54394_length_2207_cov_2.434480"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /gene="rplX"
                     /locus_tag="PLY30_02800"
     CDS             <1..200
                     /gene="rplX"
                     /locus_tag="PLY30_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPW65065.1"
                     /translation="VTKHVKPSQKNPQSGIIKQEAPIYASKVMLVCPSCGAATRVGRG
                     YLENGNKVRMCKACGEIVDQV"
     gene            215..757
                     /gene="rplE"
                     /locus_tag="PLY30_02805"
     CDS             215..757
                     /gene="rplE"
                     /locus_tag="PLY30_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPW65066.1"
                     /translation="MAPRLLEKYKNDVSPKLAKEFSYKNVMEIPRLKKVVVNIGVGDA
                     KLDIKFMDNAIAELGAITGQRPLLKRAKKSVAGFKVRQGMPVACTVTLRGERMWEFLD
                     RLLSLALPSIKDFQGVSRRGFDGRGNYNLGLKEQLIFPEISYDKVIRSRGMNVSLVTS
                     AKTDEEAQALLRELGMPFTR"
     gene            774..959
                     /locus_tag="PLY30_02810"
     CDS             774..959
                     /locus_tag="PLY30_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPW65067.1"
                     /translation="MARKAIKHKAAQPPKFAVRKYNRCPLCGRVHAFMRRFNMCRCCF
                     RKLAREGKIPGVVKSSW"
     gene            988..1392
                     /gene="rpsH"
                     /locus_tag="PLY30_02815"
     CDS             988..1392
                     /gene="rpsH"
                     /locus_tag="PLY30_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPW65068.1"
                     /translation="MYVTDPIADMLTRVRNANMVYHESVDVPMSKIKMEIARILKEAG
                     YIKNFKVVNDPKKAYSSIRLFLSYGPNRERVIQGLRRISKPGRRVYVGHADLPKVMGG
                     LGIAVISTPKGLKTDTEAGTLGLGGEVLCYVW"
     gene            1409..1948
                     /gene="rplF"
                     /locus_tag="PLY30_02820"
     CDS             1409..1948
                     /gene="rplF"
                     /locus_tag="PLY30_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPW65069.1"
                     /translation="MSRIGRKPIPVPSGTTVAVEGGAVVVKGPKGRLETPMVPGIDVA
                     VEGGIIVVTRQNEQKQTKAFHGLIRALVANMITGVSGGFKKDLEIVGVGYRAQMQGKK
                     LILNLGYSNPVEFEAPEGIELALDGPTKLSVKGIDKQAVGQVSAIIRGFRPPEPYQGK
                     GIRYAGEHIIRKAGKTGAK"
     gene            1965..>2207
                     /locus_tag="PLY30_02825"
     CDS             1965..>2207
                     /locus_tag="PLY30_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPW65070.1"
                     /translation="MKTKTRSILREIRHARLRKTLSGTVEKPRMAVFHSLKHIYVQFI
                     DDERGHTLAAASTLDPALRGTLPGTCNIETAKIIGKI"
BASE COUNT          556 a          595 c          657 g          399 t
ORIGIN      
        1 tggtgacaaa gcacgtgaaa ccctcccaga agaatcccca gtcgggcata atcaagcagg
       61 aagccccgat atacgccagc aaggtcatgc tcgtatgccc ctcctgcggc gctgcaacac
      121 gcgtcggtcg cggctatctc gagaacggga acaaggttcg tatgtgcaag gcctgcggcg
      181 agatcgtgga ccaggtgtaa ggaggtaagc gtgaatggcc cctcggctgc ttgaaaaata
      241 caaaaatgac gtctcgccga aactggcgaa ggagttcagt tacaaaaacg tgatggaaat
      301 accccggctt aaaaaagtcg tggtgaacat cggtgtgggc gacgcaaagc tcgacatcaa
      361 attcatggac aacgcgatcg ctgagcttgg cgcgatcaca ggacagcgtc cgcttttgaa
      421 gagagcgaag aaatcggtcg ccggattcaa ggtccgccag ggaatgcccg tagcgtgcac
      481 cgtcacgctg cgcggagagc gtatgtggga gtttcttgat cgtctccttt cgctcgcgct
      541 tcccagcatc aaggactttc agggcgtctc ccgccgcggt ttcgacggaa gaggcaacta
      601 caatctcggt ctcaaggagc agcttatctt ccctgagatc agttacgaca aggtcatccg
      661 ttcccgtggc atgaacgtct cgcttgtaac ctcggcgaag acggatgagg aagcccaagc
      721 tcttctccgc gagctgggaa tgccgttcac ccggtaggaa gggggagtcg aaaatggcga
      781 gaaaggcaat caagcataaa gcggcgcaac ccccgaagtt cgcggtgcga aagtataaca
      841 gatgtccgct ctgcggccgt gttcatgcct tcatgagaag attcaacatg tgccgctgct
      901 gtttcaggaa gcttgctcgc gaagggaaaa ttcccggcgt cgtcaagtcg agctggtagt
      961 cgagtggact gaagggggtc actaggaatg tacgttaccg atccgatagc ggatatgctc
     1021 actcgcgtca ggaacgcaaa catggtgtat catgagagcg tcgacgttcc gatgagcaag
     1081 ataaagatgg aaattgcccg catcctgaag gaagccgggt atattaagaa tttcaaggta
     1141 gtcaacgacc cgaagaaggc ctacagctca atcaggctgt tcctctccta cggaccgaac
     1201 cgcgagaggg tcatccaggg gctgcgccgt atcagcaaac cgggcagaag agtctacgtt
     1261 ggacacgccg accttcccaa ggtcatgggc ggcctcggca tcgccgtcat ctccacaccg
     1321 aagggcctca aaaccgacac ggaagccgga acgctcggcc tcggcggcga agtcctctgc
     1381 tacgtgtggt aaggaagagg tggagtagat gtctcgaatc ggacgcaagc caattccggt
     1441 accctccgga acgaccgtcg ccgtcgaagg cggcgccgtc gtggtgaagg ggccgaaagg
     1501 gcggctcgaa acgcccatgg ttccggggat cgacgtcgcc gtcgaaggcg gcataatcgt
     1561 cgtcacccgg cagaacgaac aaaaacagac gaaagcgttc cacggcctga tccgtgcgct
     1621 tgtcgccaac atgatcacag gcgtcagcgg cggcttcaag aaggatcttg aaatcgtcgg
     1681 cgtcggctac cgagcacaga tgcaagggaa gaagctcatc ctcaacctcg gctactcaaa
     1741 ccccgtggag ttcgaagctc ccgaaggcat cgagttagcc ctggacggac cgacgaagct
     1801 ttcggtcaag gggatcgaca aacaggcggt agggcaggta tccgcgatca ttcgcggttt
     1861 ccgtccgccc gagccgtatc agggtaaggg aatccggtac gccggcgagc atatcattcg
     1921 caaggccgga aagaccggag cgaagtaatt cgaggtgaat ggacatgaaa actaaaacca
     1981 gaagcatatt gcgtgagatt cggcatgccc ggcttcggaa aaccctctcc ggtacggtgg
     2041 aaaaaccgag aatggctgtg tttcatagct tgaagcacat ctatgttcag tttatcgacg
     2101 atgagagggg acacactctc gcggcggcgt ccacactcga tccggcactc cggggaacgc
     2161 tccccggaac ctgcaacatc gagacggcga agatcatcgg aaaaata
//
LOCUS       DAOOBX010000152         2202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_54538_length_2202_cov_3.724266, whole genome shotgun sequence.
ACCESSION   DAOOBX010000152 DAOOBX010000000
VERSION     DAOOBX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54538_length_2202_cov_3.724266"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="PLY30_02830"
     CDS             complement(<1..1638)
                     /locus_tag="PLY30_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPW65071.1"
                     /translation="MRAKLSGRRFWFLYIVLFILFGLIVYQVVQLTYFHKLSLVELAH
                     RQHYVTIDIPPMRGPITDRIGKEFVSNLKIPSIYAVPRLLDAAGRERLTKELAQILKL
                     DKKFVARRLERDKAFVWLKRRVTFEEAEAVKKLDNPALGFIEEYKRFYPQGSLLANVI
                     GFSNVDNQGLEGIELSMDSYLRGKAGKCVTKRDAKGREIKAFEIKMLPVINGDTVSLT
                     IDQRIQYLTEKALDAAVKKWKPKGAWVVVMDPKTGEILALANRPTFDPNHYDLSAPDS
                     RRNRAVTDMYEPGSVFKIVTASGALNEGVVTPSTTFFCENGKWNYGVKTLRDVHGYGT
                     LTMEQVIIKSSNIGTVKIGLKMKRDKLQEYIRGFGFGAYSGIDLKGEAPGYTRPPSQW
                     SKTSPYCIPIGQEVMVTALQMATALSVVANGGYLVKPYIISKVTDPAGVTIKENKPMI
                     RKVVIKPETAETMRKILIKVVEEGTGTHAKIPGVIAGGKTGTAQKVLPNGKGYSHSDF
                     MSSFIGFAPGDNPRYVMAVVLDEARPMYYGGVVAAPVF"
     gene            complement(1642..2037)
                     /locus_tag="PLY30_02835"
     CDS             complement(1642..2037)
                     /locus_tag="PLY30_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65072.1"
                     /translation="MKGSGKILSVIAGLTCLMLVYVHLQIASVIVSVELHKTSRDFSM
                     KQELLRRLQFNVEELKAPRLLEEKMKNHDLALGLPKKVEVVEVPAVPEFFLSQTAVPE
                     KADPAPASSISKFFGHLIQTAQAKTELSS"
     gene            complement(2034..>2202)
                     /gene="mraW"
                     /locus_tag="PLY30_02840"
     CDS             complement(2034..>2202)
                     /gene="mraW"
                     /locus_tag="PLY30_02840"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HPW65073.1"
                     /translation="FHSLEDRIVKEKFRELAAKNIAEIVTRKPVVANRSEIIDNPRAR
                     SAKLRVIEVKP"
BASE COUNT          444 a          634 c          622 g          502 t
ORIGIN      
        1 gaaaacggga gccgcgacaa cgccgccgta atacatcggc cgcgcttcgt cgaggacaac
       61 ggccatcaca tacctcggat tgtcccccgg agcaaatccg atgaaggagg acatgaaatc
      121 actgtgggaa tagcccttcc cgttcgggag cactttttgc gcggtccccg tctttccgcc
      181 cgcgataacg ccggggattt tcgcatgcgt cccggtaccc tcttcgacga ccttgataag
      241 tatcttcctc atcgtttcgg cggtctcggg cttgatcaca accttccgga tcatgggctt
      301 gttctctttg atcgtcacgc cggcggggtc cgtgaccttt gagatgatgt agggtttcac
      361 gagatacccc ccattcgcaa cgacggagag tgccgtcgcc atttggaggg cggtgaccat
      421 tacctcctgg ccgatcggga tgcagtaagg agacgtcttc gaccattgtg aaggcgggcg
      481 cgtgtagccg ggcgcctctc ccttcagatc aatgccgctg tacgcgccga aaccgaaacc
      541 gcggatgtac tcctggagtt tgtcgcgttt catcttgaga ccgatcttga ccgttccaat
      601 attgctcgac ttgatgatca cctgctccat cgtcaacgtg ccgtagccgt ggacgtcgcg
      661 aagcgttttg acgccatagt tccatttgcc gttctcgcag aagaaggtcg tggacggcgt
      721 cacgacgccc tcgttcagcg ccccgctcgc ggtcacgatc ttgaagacgg agcccggctc
      781 gtacatgtcc gtgacagccc gattccgcct cgagtcgggc gccgaaaggt cgtagtggtt
      841 cggatcgaag gtcggccggt tcgcgagcgc gagaatctcc cccgtcttcg gatccatcac
      901 gacgacccag gcgccttttg gcttccattt cttgacggcg gcatcaaggg ccttttcagt
      961 caggtactgg atgcgttgat cgatcgtcag tgaaacggtg tcgccgttga tgaccggcag
     1021 catcttgatc tcgaaagcct tgatctcacg acctttcgcg tcgcgcttcg tgacacactt
     1081 gccggccttc cctctcaggt aggaatccat gctgagctcg ataccctcga gcccctgatt
     1141 atcgacgttc gagaaaccga tgacgttcgc gaggagcgaa ccctgcgggt agaatcgctt
     1201 gtactcttcg atgaagccga gcgcggggtt atcgagtttt ttgaccgcct ccgcctcttc
     1261 gaaggtcacg cgccgtttca accagacaaa agccttgtcg cgttcgagac gcctcgcgac
     1321 gaacttcttg tcaagcttga ggatttgcgc gagttctttc gtcagccttt cgcgtccggc
     1381 cgcgtcaaga agtctcggca cggcgtagat cgaaggaatc ttgaggttcg agacgaactc
     1441 cttgccgatc cggtccgtga tcggaccgcg catcggagga atgtcgatcg tgacatagtg
     1501 ctgccggtgg gccagctcaa cgagcgacag cttgtggaaa tacgtgagct gaacgacttg
     1561 atagacgatg agcccgaaga ggatgaaaag aacgatgtaa aggaaccaga aacgacgtcc
     1621 cgaaagcttc gcccgcatgc ttcaggatga gagttcggtc ttcgcctgcg cggtctggat
     1681 caaatgaccg aagaacttcg agatcgacga ggcaggcgcg ggatccgcct tttcgggaac
     1741 cgccgtctgg gacaggaaga actccgggac cgcgggcacc tcgaccacct cgactttttt
     1801 cggaaggcca agcgcgagat cgtggttctt cattttctcc tcgaggaggc ggggggcctt
     1861 gagttcctcg acattaaact ggagacggcg cagaagctct tgcttcatcg aaaaatcgcg
     1921 ggatgttttg tgcagttcga ccgacacgat cacggacgcg atctgcagat gcacgtaaac
     1981 aagcatcaag caggtcaaac ccgcgatcac actcagaatt tttccgctgc ccttcatggt
     2041 ttgacctcaa tcactcttag tttcgcgctg cgcgcacgtg gattatcaat gatttccgaa
     2101 cgatttgcaa caacgggctt gcgcgtcacg atttctgcga tgtttttggc ggcaagttcc
     2161 cggaattttt ccttcacgat ccggtcctcg agcgaatgga ag
//
LOCUS       DAOOBX010000153         2175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_55393_length_2175_cov_3.480189, whole genome shotgun sequence.
ACCESSION   DAOOBX010000153 DAOOBX010000000
VERSION     DAOOBX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55393_length_2175_cov_3.480189"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLY30_02845"
     CDS             <1..619
                     /locus_tag="PLY30_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW65074.1"
                     /translation="VTNLWARGIIGCNPIWKLAVTGRSRIEKGKHYIVIANHQSMLDI
                     LVVAAALPLHFKFLAKKELFSIPFLGWAMSCAGYIPVDRASHESGRRAMKRIQALLAK
                     GLSVLLFPEGTRSPDGKIHGFKAGAFKLARESGVEILPVVIDGTGSALPKRSLVLRNR
                     TRFVVSIGKPVWFATLNDRSLDEARESVRHEMVARLARLRHGRQL"
     gene            750..1487
                     /locus_tag="PLY30_02850"
     CDS             750..1487
                     /locus_tag="PLY30_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPW65075.1"
                     /translation="MKILTLNTWQERGPWKERWELIFRGLKDYDPDIVGFQEVFNMDW
                     AAEIQKRSGYPFLAVSGEHSGLVFLSRFRPVEQSCLIYRTKSPNEDYLRYGFYVRVEA
                     PSGPVPLFNTHLSWQASDHAIRMSQVLELVEFIKLKAGTRPSAVMGDFNTAPDTLPVV
                     FLRDSQMWTDTYAAANPGEAGLTWDYRNPYAAMERDKMAERRIDFVFVTEREGAFAKI
                     KTSKVVFDAPGGNIWPSDHFGVMTEFE"
     gene            1484..>2175
                     /locus_tag="PLY30_02855"
     CDS             1484..>2175
                     /locus_tag="PLY30_02855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF027806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPW65076.1"
                     /translation="MINDMNMTVKDNGKAKEYQAIRRKLTLVHLALTPGLLAVMLLSG
                     FHLVLRDLTAAVAGGNEWMRVAVYFSLFSVYFLVFDLPLSFYSGFRLEHRFGLSNQTL
                     GAWVSDFLKRSFLSFALSLALIELLYFILRAAPGTWWLWAWAGYAFVSYILGKIFPVV
                     IVPLFYKYGKLDDETLAQKVKALAARFGMPVGNVYSLNLSKTTRKANAAFMGIGRTKR
                     VVLSDTLISNFT"
BASE COUNT          422 a          603 c          651 g          499 t
ORIGIN      
        1 ggtgacgaat ctctgggccc ggggcatcat tggctgcaat cccatctgga agcttgccgt
       61 gacgggacgc tcccgcatcg aaaagggcaa gcattatatc gtgatcgcga accaccagag
      121 catgctcgac atcctcgtgg tggccgccgc gctgccgctc catttcaagt ttctcgcaaa
      181 aaaagaactt ttcagcattc cgttcctcgg ctgggctatg agctgcgcgg ggtatatccc
      241 cgtggaccgc gcgagccacg aaagcggccg tcgcgcgatg aagcggattc aggccctcct
      301 cgcgaaaggt cttagcgtcc ttcttttccc cgagggtacg cgcagcccgg acgggaaaat
      361 ccacgggttc aaggcgggcg cgtttaaact cgcgcgtgaa agcggggtcg agatcctccc
      421 ggttgtgatc gacggcaccg gaagcgcgct tccgaagcga agtctcgtcc tgaggaaccg
      481 gaccaggttc gtggtgtcga tcggtaaacc cgtctggttc gcgacgctca atgaccgatc
      541 gcttgacgag gccagggaat ctgtgcggca tgagatggtc gcccgtctcg cgcggctgcg
      601 ccacggacgg cagttatgag tacggagtac ggagtacgaa gacgtttctg cggggggccg
      661 gcccgggcat tccgggtttc ccggggtgag gttgtttttc cgtgcttcgt atgccgtgtc
      721 ccgcactttc catgcgaggc cggccgggca tgaagattct caccctgaac acctggcagg
      781 aacgcggacc gtggaaggag cggtgggaac tcatcttccg cggattaaag gactatgatc
      841 cggatatcgt cgggttccag gaagtgttta atatggattg ggccgcggag atccaaaagc
      901 ggtccggata ccccttcctc gcggtgagcg gcgaacactc cggtcttgtt tttctctcac
      961 gattcaggcc cgtcgagcag tcgtgcctca tctaccggac gaaatccccg aacgaagact
     1021 atctgcgcta tggattttat gtccgcgttg aagcgccgtc gggaccggtc ccgctcttca
     1081 acacgcacct gtcgtggcaa gcttccgatc acgccatccg catgagccag gtgctggagc
     1141 ttgtggagtt catcaaactc aaagccggca cacgcccctc cgcggtcatg ggggacttca
     1201 ataccgcgcc tgacacgctt ccggtcgtgt ttctccgtga ttcgcaaatg tggacggata
     1261 cttacgcggc ggcgaatccg ggcgaggccg gcctgacgtg ggattacagg aatccatacg
     1321 cggcgatgga acgcgacaag atggccgagc ggaggatcga ttttgttttc gtgacggaac
     1381 gcgaaggcgc tttcgcgaag atcaaaactt caaaggttgt ttttgacgcc cccggcggaa
     1441 acatctggcc gtcggatcat tttggggtga tgacggagtt tgaatgataa atgatatgaa
     1501 tatgaccgtt aaggataacg gtaaagcaaa agaatatcag gcgatccgca ggaaattgac
     1561 gcttgttcat ctcgcgctga cgcccgggct tctcgcggtc atgctcctgt cgggtttcca
     1621 cctcgttttg agggacctca cggccgcggt cgccggcggg aacgaatgga tgagggtcgc
     1681 ggtctacttt tccctgttct cggtttattt tctcgtcttc gatcttccgc tctcgttcta
     1741 ttcaggattc cggctcgaac accggtttgg tctttccaat cagaccctgg gagcctgggt
     1801 ttccgatttc ctgaaaagat catttctttc attcgccctt tcgcttgccc tgatcgaact
     1861 gctctatttt atcctccgcg ctgctccggg gacctggtgg ctctgggcct gggcgggtta
     1921 tgccttcgtg agttacattc tcggaaaaat tttcccggtc gtgatcgttc cgctgttcta
     1981 caagtacggc aagctcgatg atgaaacgct cgctcaaaag gtgaaggcgt tggccgcgag
     2041 gttcgggatg ccggtcggga acgtctattc gctgaatctc agcaagacga ccaggaaggc
     2101 caatgccgcg ttcatgggga tcggcagaac gaaacgggtc gttttgagcg atacgctgat
     2161 ctcgaatttc accca
//
LOCUS       DAOOBX010000154         2171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_55546_length_2171_cov_2.909263, whole genome shotgun sequence.
ACCESSION   DAOOBX010000154 DAOOBX010000000
VERSION     DAOOBX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55546_length_2171_cov_2.909263"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="PLY30_02860"
     CDS             complement(<1..2071)
                     /locus_tag="PLY30_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65077.1"
                     /translation="MATNVAPVVDPVRDFEAMRQDLLKRIEQLPEDSRERKQFETEIR
                     SAPTVEVLMRISQKIAPALASLAMATATPMGTPGAGGPPPAAEATQGSVAFPAAAVSS
                     TAKQTTSPAAGSLILTETQDIRDRENARIAALAKERADREAAELLSKAFEPAISEPAL
                     SPYDRAMKDFSSFKESAARDIKYPKYRQRFLDDAAELEKTMRTPADIRRAHRALEVFA
                     GKIMAASVSPAPDAAMKPDEIAMRADILRNYRIPGALGELTAARDAEKPDAKKVEELR
                     KNYSDLKLELGRYDVLIKAMEAPSEEETPVAVEAVKATPEEVTRLRAEMDQILAALTD
                     QNDKDLVRNEASRLMNQLNEGTITFADATQRLTDYSLPFYQKMETPASESFWDRLFGP
                     SMGSERKAAIDSYKKFIRDTVAGIKDAGVRADVQKFIKTGLPEASAIKNDSDLAAAQK
                     KLTDHVAKVTAEKAARLERALAEIPAEPLLRDAAGEPATETPAPVTAGPDAVGVVISE
                     DLMSGKPSIEKALGDVASGTVQSGPNALGANAAKGQLKLDPAKIREAINNSQMFRNQK
                     FNIDIAKEKKHGAFQPFIVTLNISYDFMAYSDQNGIYGHDKGFFYIEGSKHGGSRTEA
                     SAAAGKTTGSGAWELVQRREWTGRSAPATGFEQDGLVPIYDEPLTADQQAYIRVQDDV
                     RAAINAAW"
BASE COUNT          398 a          675 c          591 g          507 t
ORIGIN      
        1 cccaggctgc attgatcgcc gcgcggacat catcctggac ccttatatag gcttgttgat
       61 cagccgtaag cggctcatca taaatcggta ccaggccatc ctgttcaaaa ccggtggcgg
      121 gcgccgaacg ccccgtccac tcacgccgtt gtacaagttc ccaagctccc gatccggtcg
      181 tcttccccgc ggcggctgat gcctcagtcc tgcttccgcc gtgtttggat ccttcgatat
      241 agaaaaatcc tttgtcatga ccataaatcc cgttctgatc gctgtaggcc ataaaatcat
      301 aagaaatatt cagcgttacg ataaacggct ggaacgctcc atgcttcttt tctttggcga
      361 tatcaatatt gaacttctga ttccggaaca tctgcgagtt gttaatagcc tcccgaatct
      421 tcgcaggatc gagcttgagt tggccttttg cggcattcgc cccaagtgcg ttcggccccg
      481 actgcaccgt tcccgaggcc acgtcgccaa gggctttttc gatcgacggc tttccactca
      541 tcaaatcttc ggaaataacc acacccacag cgtcgggacc ggccgttacc ggcgcgggag
      601 tttcggttgc gggctcaccg gcggcatcac gaagaagcgg ttctgccggg atctcggcga
      661 gcgccctctc gagcctcgcg gccttttcgg ccgttacttt cgcgacatga tcggtcagtt
      721 tcttttgggc ggcggcaaga tcactatcat tcttgatcgc ggaggcctcc gggagtccgg
      781 tcttgatgaa tttctgaaca tccgcgcgaa ctccggcgtc cttgatccct gccaccgtat
      841 ccctgataaa tttcttgtag ctgtcgatcg cggccttccg ttcggatccc atcgacggac
      901 cgaagaggcg atcccaaaac gactcgctcg ccggcgtttc catcttctga tagaacggca
      961 gcgaatagtc agtcaaccgt tgcgtcgcgt cggcaaacgt gatcgttccc tcgttcaact
     1021 ggttcatcag cctggaggcc tcattgcgaa caaggtcttt atcattctga tccgtcagcg
     1081 ccgccaggat ctgatccatc tctgcgcgaa gtcgcgtgac ttcctccgga gtggccttca
     1141 ccgcttcaac ggctacaggc gtctcctcct ccgaaggggc ctccatggcc tttataagga
     1201 cgtcgtaccg gccaagctca agcttcagat cactgtaatt tttccggagt tcctcgacct
     1261 ttttggcatc cggtttttcc gcgtcccgag ccgcggtcaa ctcaccgaga gctccgggga
     1321 tgcggtaatt cctgaggatg tcggcccgca tcgcgatctc gtcgggcttc atcgccgcgt
     1381 caggggcggg actcacggaa gcggccatga tcttgccggc aaaaacctca agagccctgt
     1441 gagcgcgcct gatatcagcg ggagtgcgca tggtcttttc cagttccgcg gcgtcatcaa
     1501 ggaatctctg gcggtacttg ggatacttga tatcgcgtgc cgcggattct ttaaaagagc
     1561 tgaagtcctt catcgctctg tcatagggcg atagcgcggg ctcggaaatc gcgggctcaa
     1621 aagccttgct gaggagctcg gccgcttcgc gatccgcgcg ttctttggcg agcgccgcga
     1681 tcctggcatt ttccctgtcc cggatatctt gcgtctcagt aaggatcagg gaccctgccg
     1741 ctggcgaggt cgtctgcttc gccgtggagg aaaccgctgc cgcgggaaac gctacggacc
     1801 cttgcgtggc ttctgccgcg ggcggcggac cgccggcgcc cggcgtcccc atcggagtcg
     1861 cagtagccat cgcgagcgac gcgagagcag gagcgatctt ttgactgatc cgcatcagca
     1921 cttctaccgt cggggcactc cggatctcgg tttcaaactg cttccgttcc ctggaatcct
     1981 cgggcaactg ctcgatccgc ttcaggaggt cctggcgcat cgcctcgaaa tcccggaccg
     2041 gatcgaccac aggggcaaca tttgtggcca ctccaggtga ctggaaaagg ctgcgcagat
     2101 tatcaaggaa ctgctcccgc gatttcgcct caagttccgc ctcatacata ctgttcgtga
     2161 cgatcgcggc c
//
LOCUS       DAOOBX010000155         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_55957_length_2160_cov_2.924941, whole genome shotgun sequence.
ACCESSION   DAOOBX010000155 DAOOBX010000000
VERSION     DAOOBX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55957_length_2160_cov_2.924941"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PLY30_02865"
     CDS             complement(<1..1141)
                     /locus_tag="PLY30_02865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW65078.1"
                     /translation="MADERESRRIRAQLRVAAGLWSGAQKESIPADRFLSRYFFHHRK
                     KTGARDRRFLSETIFGAFRHKSFLEAWLESGRGFAQPSPEGAERDVLFCSLAAAKEHL
                     IGKEFFDGTGLLEGVYEALQDQILPEGCAGLPEDEKHALLYSFPLWLVKRWRERYGAE
                     KAEKLCSCFLSRPRPILRTNTLKTTREKLIGALCRMGVKAEAAPRSPFGVILRERRNV
                     LDSPAFRDGWFELQDEGSQLVCLKMDAKPGETVWDVCAGGGGKSLMLAAMMENKGRIV
                     ATDLRGYKLDVLKKRAKRAGAFNIFPVNLDRMSELAVARKGFDRILVDAPCSGTGTMG
                     RNPDAKWRIDETWFARLHEEQAGILEKALPYLKKNGKLYYATCSLE"
     gene            complement(1248..1688)
                     /locus_tag="PLY30_02870"
     CDS             complement(1248..1688)
                     /locus_tag="PLY30_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65079.1"
                     /translation="MKKFLSCILVLFLVTVFTVPSAFAAGGKEFGASLLLPTTGQTMN
                     GQFGSTKTKLMAGIEVAAITATVILGFVPGGAIVWAGAGPLLANHLWSATDAFVTARK
                     KDNDPYVQEQMLEAQRTLDVSRQNRFARESNIRQRILKAGEESY"
     gene            complement(1762..>2160)
                     /locus_tag="PLY30_02875"
     CDS             complement(1762..>2160)
                     /locus_tag="PLY30_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPW65080.1"
                     /translation="AATLTGLAHLTFGDKTCAILGTDQKLIDTIREAGEKTGERCWQL
                     PLWDEYADAIKGNHSDINNTGDGYAGTITATMFLKEFVPAKVPWVHLDIAGTAYIGKN
                     NHECPKGATGFGVRLFTEVLSNWSRESFSK"
BASE COUNT          426 a          687 c          576 g          471 t
ORIGIN      
        1 gctccagcga gcaggtggcg tagtacagtt tgccgttttt cttcaggtat ggcaaagctt
       61 tttcgagaat cccggcctgc tcttcatgaa gccgcgcgaa ccaggtctcg tcgatcctcc
      121 acttggcgtc cgggttccgg cccatcgttc ccgttccgga gcatggggca tcgaccagga
      181 tccggtcgaa ccccttccgg gcgaccgcca gttccgacat gcgatcgagg ttcaccggga
      241 agatattgaa cgctcccgcg cgcttcgcgc gttttttcaa cacatcaagc ttgtaaccgc
      301 gaaggtccgt cgccacgatc cggcctttgt tctccatcat cgcggccagc atgagactct
      361 tcccgccccc acccgcgcag acatcccaga ccgtctctcc cggtttcgcg tccattttca
      421 gacagaccag ctgacttccc tcgtcctgga gttcgaacca tccatcccgg aaagcggggc
      481 tatcgagcac gtttctgcgt tcccgtaaaa tgaccccaaa aggagaacgg ggtgccgctt
      541 ccgccttcac acccatccgg caaagggcgc cgatcagttt ttcgcgggtg gttttgagcg
      601 tgttcgtccg gagaatcggt cgcggccggc tcagaaaaca gctgcatagc ttttcggcct
      661 tttcggctcc gtaccgctct cgccatcgct tgacgagcca gagcgggaac gaataaagaa
      721 gggcgtgttt ttcatcctcc ggaagtcccg cacagccctc cggaagaatc tggtcctgaa
      781 gcgcctcata gacgccttcc aagaggccgg tcccgtcaaa gaactccttt ccgatcaggt
      841 gttccttcgc cgccgcgagc gagcaaaaga gcacgtcccg ctccgctccc tccggggacg
      901 gttgcgcgaa accccgccca ctttcaagcc atgcctcaag aaagctcttg tgccggaatg
      961 cgccaaaaat cgtttcggac aggaaccggc ggtcgcgagc gcctgttttt tttcgatgat
     1021 gaaaaaaata acggctcaga aagcgatcgg cgggtatcga ttccttttgc gccccgctcc
     1081 aaagcccggc ggcaacacgc agctgcgccc tgatacgcct gctctctctt tcatccgcca
     1141 ttctggacac ccctcacgcc ccgtgccgtc acgaaggtct ttaaaagtaa aaaggcctga
     1201 tcggggagat caggcctttt ttggatctac gggtcccaac aataaactca gtaactctcc
     1261 tctccggcct tgaggatgcg ctggcggata ttcgattcgc gcgcgaaacg gttctgccgc
     1321 gaaacatcca gtgtccgctg cgcctcaagc atctgttcct gcacgtacgg gtcattgtcc
     1381 ttcttgcggg cggtgacgaa ggcgtccgtc gcgctccaca gatggttggc aagcaacggc
     1441 cccgcgccgg cccaaacgat cgcgccgccc ggaacgaaac cgagaatgac ggtcgcggtg
     1501 atcgctgcca cctcgatgcc ggccatcagc ttggtcttgg tgctcccaaa ctggccgttc
     1561 atcgtttggc cggtggtcgg caacagaaga gaagcgccaa actctttacc acccgccgcg
     1621 aacgccgaag gaaccgtgaa aacggtgaca aggaataata ccaaaatgca ggacaggaat
     1681 ttcttcataa ttcggtctcc ttggattgag gtgtttccgc cgtatcatta acgtcctgaa
     1741 agccgaaaat cttaacctct gttattttga gaaactttcc cggctccagt tcgagagcac
     1801 ttcggtgaaa agcctgaccc cgaaccccgt cgcgcccttg ggacattcgt gattattctt
     1861 cccgatataa gccgttcccg cgatgtcgag atggacccat ggcaccttcg ccgggacgaa
     1921 ctccttgaga aacatcgtcg cggtgatggt ccccgcgtaa ccgtcgccgg tgttgttgat
     1981 gtccgagtgg ttccctttga tcgcatccgc gtactcgtcc cagagcggca gctgccagca
     2041 acgctcgccg gtcttctcgc ccgcttcgcg gatcgtatcg atcagttttt gatcggtacc
     2101 gaggatcgcg caggtcttgt caccgaaggt caggtgggcg agtcctgtca gcgtggccgc
//
LOCUS       DAOOBX010000156         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_56943_length_2134_cov_2.382876, whole genome shotgun sequence.
ACCESSION   DAOOBX010000156 DAOOBX010000000
VERSION     DAOOBX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56943_length_2134_cov_2.382876"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2126
                     /gene="dnaE"
                     /locus_tag="PLY30_02880"
     CDS             <1..2126
                     /gene="dnaE"
                     /locus_tag="PLY30_02880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPW65081.1"
                     /translation="AVIRDVGRVAGLSYGEADTIAKLITNELNITIDQAIEKEPALKE
                     QIAKDSRIAKLIETAKALEGLSRHASVHAAGVVISDVPLTDRVPLFKAEDQICTQFAM
                     KDLEKIGLLKMDFLGLKTLTVINQAVKIIRKMHDVALDIHNLPEDDPETYEMLARGDA
                     FGVFQLESSGMRDLLKKMKPSKFDHLVALLALYRPGPLGSGMVDNFVERMHDPKLIKY
                     DHPGLEPSLKETYGVILYQEQVMRIVSDLAGFTLAQADSLRRAIGKKIPEVMAKEKKA
                     FIDGAMKRGVKEKVAEKIWDQIDYFSGYGFNKSHSTAYAAVSYQTAYLKAHYPVEFMT
                     ALLTSEMGNTDKVVQYIDASKKMGIEVLPPSVNESFKEFTCVGKNIRFGLAAIKNVGA
                     TAIESVIASRTQNGSFKNFFEFVQRVDLRVCNRKVLESMIKCGAFDEWKLHRSQLMAM
                     LDTAIEMGSNLQKDRNRGQMSFFESGGEGGAFHDENAAIPDIPEWPEGQSLAFERELL
                     GFYVSSHPLSKYAKILKNYASATSDTLSDFNHQAEVTMGGVVDQLKEILTKKGDKMAF
                     VTLQDLLGSCEVIVFQNVYKEVSNLIQKDRLIFVRGKIDARDDTPKILADEIVPLGEV
                     PRRFTKLVAISLKTAGLVPDTLKEVRRILMKHKGAVPVYISLMDPKGRRTVLDSGENL
                     KVETSDALFEELEHLLGENSVKIRS"
BASE COUNT          481 a          592 c          645 g          416 t
ORIGIN      
        1 aggccgtgat ccgcgacgtc gggcgcgtgg ccgggctttc ctacggcgaa gcggacacga
       61 tcgcgaaact gatcacgaat gagttaaaca tcacgatcga ccaggctatc gaaaaagagc
      121 cggccctgaa agagcagatc gcgaaggact cgcggatcgc gaaactgatc gaaacggcca
      181 aggcgctcga aggcctctcg cgtcacgcct ccgtccatgc cgcgggtgtc gtgatctcgg
      241 acgtgccgct cacggatcgg gtcccgcttt tcaaggccga ggaccagatt tgcacccagt
      301 tcgcgatgaa ggacctcgag aagatcgggc tcctgaagat ggatttcctc ggcctcaaga
      361 cgctgaccgt catcaatcag gcggtcaaga tcatcaggaa gatgcacgat gtcgcgctcg
      421 atatccacaa cctgcctgag gatgacccgg agacctacga aatgctcgcg cggggggacg
      481 cgttcggtgt gttccagctg gaaagttcgg ggatgcgcga tctcctcaaa aaaatgaagc
      541 cgtcgaagtt cgatcacctc gtggccctcc ttgcgctcta ccgcccgggg ccgctcggca
      601 gcggcatggt ggacaatttt gtggagcgta tgcatgaccc gaagctgatc aagtatgacc
      661 atccggggct tgagcccagt ctgaaggaga cctacggcgt catcctgtac caggaacagg
      721 tcatgcggat cgtgagcgac cttgccgggt tcacgctcgc gcaggccgat tcgctccgcc
      781 gcgcgatcgg caaaaaaatc ccggaggtta tggcgaagga aaagaaagcc tttatcgacg
      841 gtgccatgaa acggggtgtg aaggagaaag tcgccgagaa gatctgggac cagatcgatt
      901 acttttcggg gtacgggttc aacaagtccc attccacggc gtatgccgcc gtatcgtacc
      961 agacggcgta cctgaaggcc cattatccgg tggagttcat gacggcactg cttacgtccg
     1021 aaatgggaaa cacggacaag gttgttcagt acatcgatgc ctccaagaag atggggatcg
     1081 aggtccttcc gccttccgtg aacgaaagtt tcaaggagtt cacgtgtgtc ggcaagaaca
     1141 tccgtttcgg gctggccgcg atcaagaatg tgggggcgac cgcgattgaa tccgtgatcg
     1201 cgagccgtac gcaaaacggc tccttcaaga acttcttcga gttcgtccag cgcgtcgatc
     1261 ttcgcgtctg caaccggaag gtcctcgaga gcatgattaa gtgcggagcg ttcgacgaat
     1321 ggaagctcca ccgctcgcag ctgatggcca tgctcgacac ggccatcgag atgggttcaa
     1381 acctccagaa ggatcgcaac cgcggtcaga tgtccttttt cgagagcggg ggagagggcg
     1441 gggcctttca cgacgagaat gccgcgatcc cggatatccc cgaatggccg gagggacaga
     1501 gccttgcgtt cgaacgcgaa ctgctcggat tctacgtctc ctcgcacccg ctctccaagt
     1561 acgcgaagat cctgaagaac tatgcctcgg ccacttccga cacgctttcc gacttcaacc
     1621 accaggccga ggtcacgatg ggcggcgtgg tcgatcaact gaaggaaatt ctcacgaaga
     1681 aaggggacaa gatggcgttt gtgacgcttc aggaccttct cgggtcctgc gaggtcattg
     1741 tgttccagaa cgtctacaag gaggtgtcga atctcatcca gaaggaccgg ctgatcttcg
     1801 tgcgcgggaa gatcgacgca cgggacgaca ccccgaagat tcttgccgac gagatcgttc
     1861 ccctggggga agtgcccagg cgttttacga aacttgtggc gatcagcctg aagacagcgg
     1921 ggctcgttcc tgacaccctg aaggaggtcc ggcggattct tatgaaacac aagggtgcgg
     1981 tgccggttta catcagcctc atggacccga aggggcggcg cacggttctc gattccggag
     2041 agaatctcaa ggtcgagacg tcggatgctc ttttcgaaga gctcgagcat cttctcggtg
     2101 agaattccgt caagatccgt tcctgatgtc ctat
//
LOCUS       DAOOBX010000157         2124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_57286_length_2124_cov_3.428710, whole genome shotgun sequence.
ACCESSION   DAOOBX010000157 DAOOBX010000000
VERSION     DAOOBX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57286_length_2124_cov_3.428710"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLY30_02885"
     CDS             complement(<1..774)
                     /locus_tag="PLY30_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW65082.1"
                     /translation="MNPSAFDVVIIGAGGAGLTAAIYTARANLSTLILEKMVPGGQIV
                     STELVENYPGFQDGIMGAEIAQRMEAQAARYGARLDSGEVNRIFRQDGHFQIEAGGER
                     YQARAVILAMGASFRTLGVPNEKEFTGKGVSYCATCDGAFFRGKELVVVGGGDSALQE
                     GIFLTKFAAKVSIVHRRDKLRASPILQERALENPKIRFIWDTVIDKIEGAGKVESVLL
                     RNVKTQALETFGTDGVFIFIGHEPNSGIAKGFVATDDHGY"
     gene            complement(779..1183)
                     /locus_tag="PLY30_02890"
     CDS             complement(779..1183)
                     /locus_tag="PLY30_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPW65083.1"
                     /translation="MIELVLSKIKVDENRHEQLVVFREKDGNRYLPLAIGISEINSIK
                     LKLSGIIPPRPMTHDLLLNVLEGLGARLMYIVIDKIEKNTFHAKLFIRKENGEEVLID
                     ARPSDSIAVALRSGVSIFAEENVLDAAGVTEV"
     gene            complement(1176..>2124)
                     /locus_tag="PLY30_02895"
     CDS             complement(1176..>2124)
                     /locus_tag="PLY30_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65084.1"
                     /translation="EIVQNEKKRKEKEVQEIQVLLAHAEEKHESLREEFRQTRQYFEE
                     KIKANEKRSEELLKQIEERDFASRELIEEKQMLQKSFHMAKSDILRARDDQERSEKES
                     RERIAALMREIETQKKIVVELNEKIALIEEGKRFVQTSEQRLQKDLKNQEQMTKRWEA
                     KAFELEHESLKMRETFEAQMKTWQVRLAQLEAREHELEKARRQARDLQQQLDQRESVQ
                     RRRELNDAHSAKEAQLKGLVKQQEKLEGEIRHREEELRKLLHEQEAVERELIEIKQAQ
                     RHLADKAKKESLPRPLTDLSTTSDSDIFPPQTLPETRHD"
BASE COUNT          417 a          656 c          509 g          542 t
ORIGIN      
        1 ataaccatga tcatctgtcg ccacaaatcc tttcgcgatc cctgaattgg gttcgtgccc
       61 gataaagatg aacacgccat cggttccgaa ggtttccagt gcctgcgttt tgacgtttcg
      121 aaggaggacc gattcgactt ttccggcgcc ttcgatttta tcaataacag tgtcccaaat
      181 aaagcggatc ttgggattct cgagcgcgcg ttcctgaaga atcgggctcg cccgaagctt
      241 gtcacggcga tgaacgatcg agaccttggc cgcaaacttc gtcaggaaga tcccttcctg
      301 gagggccgaa tcgcccccgc cgaccacgac gagctctttg ccccgaaaaa acgcaccgtc
      361 acaggtcgcg cagtaggaaa cgcctttgcc ggtaaactct ttttcgttcg gtacccccag
      421 cgtgcgaaac gaagccccca tggcaagaat aacagccctt gcctgataac gctccccgcc
      481 cgcctcgatc tgaaaatgtc cgtcctggcg aaagatgcga ttgacctctc cggaatccag
      541 acgcgcgcca tacctggcgg cctgcgcttc catccgttga gcaatctccg cgcccatgat
      601 accgtcctga aaacccggat aattttcaac gagctccgtg gagacaatct gcccgccagg
      661 aaccattttt tcgaggatca gcgtcgaaag attggcacgc gccgtataaa tcgcggccgt
      721 caaccccgca ccgcccgctc cgataatcac cacatcaaag gccgaaggat tcatggactc
      781 aaacctccgt cacgcccgcg gcgtccagga cattctcttc tgcaaaaatt gatactccgg
      841 accgcagcgc cacggcaatg ctgtcggagg gacgcgcgtc aatgaggact tcctcgccgt
      901 tctctttccg gatgaaaagc ttggcgtgga acgtgttctt ctcgatcttg tcaatcacga
      961 tatacatgag gcgcgctccg agcccttcca gaacattcaa cagaagatca tgggtcatgg
     1021 gccgcggcgg tatgatgccg ctcagcttca acttgatgga attgatctcc gagatgccga
     1081 tcgcgagagg aaggtaacgg tttccgtcct tttcccgaaa aacgacaagt tgttcgtgcc
     1141 ggttctcatc caccttgatc ttgctaagaa ccagctcaat catggcgggt ctcgggcagc
     1201 gtctgaggag ggaaaatatc cgaatccgat gtcgttgaaa ggtccgtcaa cggacgcggg
     1261 agcgattctt tttttgcctt atccgcaagg tgccgctgcg cctgtttgat ctcgatcagc
     1321 tcacgctcca cggcttcctg ttcgtggagc aacttccgaa gctcctcctc gcgatgtcga
     1381 atctcgcctt cgagtttttc ctgctgcttc actaatcctt ttagctgcgc ctctttggcc
     1441 gagtgagcat cattgagctc gcgcctcctt tggaccgatt cccgctggtc aagttgttgc
     1501 tggagatcgc gggcctgcct ccgggccttc tccagctcat gttcccgagc ctccagttgg
     1561 gccaaccgca cttgccacgt cttcatttga gcctcgaaag tctccctcat cttcagggac
     1621 tcatgctcaa gctcgaaggc cttcgcttcc cagcgcttcg tcatctgttc ctggtttttg
     1681 agatcctttt gcaaccgttg ttcactcgtt tgaacgaacc gcttcccctc ctcgatcagg
     1741 gcgatttttt cgttcagttc cacgacgatc tttttctgtg tttcgatttc acgcatgagc
     1801 gccgcgatcc gctccctgga ttccttctcg gaacgctcct ggtcgtcacg cgcgcgcagg
     1861 atatcgctct tggccatatg gaaactcttc tgaagcatct gtttctcttc aatcagttcc
     1921 cgactcgcaa aatcacgttc ctcgatctgt ttcaggagct cttccgaccg cttctcattc
     1981 gctttgatct tttcttcaaa atattggcgg gtctggcgaa actcctcgcg gagtgattcg
     2041 tgcttctcct cggcgtgggc aagcaacacc tggatctcct ggacctcttt ctccttccgt
     2101 ttcttctcgt tctggacgat ctca
//
LOCUS       DAOOBX010000158         2122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_57352_length_2122_cov_2.815191, whole genome shotgun sequence.
ACCESSION   DAOOBX010000158 DAOOBX010000000
VERSION     DAOOBX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57352_length_2122_cov_2.815191"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1532)
                     /locus_tag="PLY30_02900"
     CDS             complement(75..1532)
                     /locus_tag="PLY30_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW65085.1"
                     /translation="MSKGKRILVAEKDEAIRELVAMRLESRHHKVFQAAKSAQVLRFI
                     EREAIDLIILSTDMEPVDGKLLIQVIRAKSHLVAVPIIMLTTEDELTEIMLGHEKGFD
                     DFIVKPFSPLVLQIRVDLNIAKTQERVEANALTHLPGNHAIEKIVSKKIKAGEKFSVL
                     YLDINHFKSFNDHYGFQKGDDVIIQTARLLIATSYAVSRDGECFVGHVGGDDFIVVTP
                     PELEDVFARQFLDDFDRVMPTYYSKEDREAGRIRVENRRGEKEDFPLMSCSVAACTNL
                     YKNYTNLREIAQDAVEVKSFLKSQPGSHYLRDRRSMPIRKVDEAVHILEPEIKLSSRH
                     HKKIKTDPLGQLLLDVGLINEEQLAGALKKHFETGQRLGQTLINMNLISTRDIGRMLE
                     KKLKIPYFCLADWQPDARLLALFTPDLIMRHRILPVAVSEESMDLAMCDPFDIRTLDM
                     IERVTGLKPVPHLALDDEFEQFLETHFKSSEKATS"
     gene            complement(1529..>2122)
                     /gene="panB"
                     /locus_tag="PLY30_02905"
     CDS             complement(1529..>2122)
                     /gene="panB"
                     /locus_tag="PLY30_02905"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPW65086.1"
                     /translation="HTRPVAKAAKRALVVTDMPYQSFLNPEEALANAQLLTGDCGADA
                     VKIEGGVGIERQVRLLVDRGVPVMGHIGMLPQGVANGKFRVYGKTGEEAEKILEDARL
                     LDRLGVFAIVLECLPAELGRRITAEVRCPTIGIGAGPGTDGQILVCHDMIGIRSSVSP
                     KFVRRYADVEDSIRKAAEQYVKDVTQGKYPSDKESFR"
BASE COUNT          394 a          650 c          611 g          467 t
ORIGIN      
        1 cgcggaccgg aacaaacaca ccacttccaa cctgcgaggt tggatcggtc gcgggttttg
       61 attcccggtt tctttcagga cgttgctttt tctgaagatt tgaaatgggt ctcgaggaac
      121 tgttcgaact catcatcgag cgcgaggtgc gggacgggct tgagacccgt cacccgctcg
      181 atcatgtcga gggtccgaat atcgaaagga tcgcacatgg cgagatccat ggattcttcc
      241 gatacggcaa ccgggaggat ccggtgccgc ataatcaggt cgggcgtgaa aagagcaagc
      301 aacctcgcgt ccggctgcca gtccgcgagg cagaaataag gtatcttgag ctttttttcg
      361 agcatccggc cgatatcccg ggtcgaaatg agattcatat tgatcagggt ctgcccgagc
      421 cgctgtcccg tctcgaagtg cttcttcagg gccccggcaa gctgttcctc gttgataagc
      481 cccacgtcaa gcaacagctg tccgagcggg tcggtcttga tcttcttgtg gtgtctcgag
      541 gacaatttga tctcgggctc gaggatgtgt accgcctcgt cgaccttcct gatcggcatg
      601 gaccgtcggt cccgcaggta atggctcccg ggctgggatt tcaggaagct tttcacctcc
      661 accgcgtcct gggcgatctc gcggaggttc gtgtaattct tgtagaggtt cgtgcaggcc
      721 gcgaccgaac acgacatcag ggggaaatcc tccttttccc cccgccggtt ctcaacccgg
      781 atccggcctg cctcgcggtc ctctttgctg tagtaggtcg gcatcacacg gtcgaaatcg
      841 tccaggaact gacgggcgaa gacatcctcc agctccgggg gcgtcacgac gatgaaatcg
      901 tctccgccga catgacccac gaagcactcc ccgtcgcgcg agaccgcgta gctcgtcgcg
      961 atcaggaggc gggccgtctg gataatcacg tcgtcgcctt tctggaaacc gtagtggtcg
     1021 ttgaatgatt tgaaatggtt gatgtcgagg tagagcaccg agaatttctc gcccgccttg
     1081 atctttttcg agacgatctt ctcgatcgcg tggttgcccg gaagatgtgt gagcgcgttc
     1141 gcctcgaccc gttcctgggt cttcgcaata ttgaggtcca cgcgaatctg gagcacgagc
     1201 gggctgaacg gcttgacgat gaaatcatcg aaccctttct cgtgaccgag catgatctcc
     1261 gtaagctcgt cttcggtcgt cagcatgatg atcgggaccg ccacaaggtg ggacttcgcg
     1321 cggatgacct ggatcaggag cttcccgtcc acgggctcca tgtcggtcga aagaatgatg
     1381 aggtcgatcg cttcccgctc gataaaacgc aggacctggg cgctcttcgc ggcctgaaag
     1441 accttgtgat ggcgggactc gagtcgcatc gcgacaagct cgcggatcgc ctcgtccttc
     1501 tccgcgacga ggatcctctt ccctttgctc accggaaact ctccttgtcc gaaggatatt
     1561 tgccctgcgt gacgtctttc acgtattgtt cggcggcctt cctgatggaa tcttccacat
     1621 cggcataacg gcgcacgaac ttcgggctga ccgaactccg gattccgatc atgtcgtgac
     1681 agacaagaat ctgcccgtcc gtgccgggac ccgcgccgat cccgatcgtc ggacagcgga
     1741 cctccgccgt gatccgccgg ccaagttccg ccggcaggca ttcgagcacg atcgcgaaca
     1801 ccccgagcct gtcgagcaag cgggcatcct caaggatttt ctccgcctct tcgccggttt
     1861 ttccgtaaac ccggaatttc ccgttcgcga ccccctgcgg gagcatcccg atatgcccca
     1921 tgacggggac gccccggtca acgagcagcc tgacctggcg ctcgatcccg accccgcctt
     1981 cgatcttgac ggcatccgcg ccgcaatccc cggtcaaaag ttgcgcgttc gcgagggctt
     2041 cttccgggtt caaaaaggat tggtacggca tgtccgtaac cacgagggcc cgcttcgccg
     2101 cctttgcgac cggccgcgtg tg
//
LOCUS       DAOOBX010000159         2120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_57435_length_2120_cov_2.477482, whole genome shotgun sequence.
ACCESSION   DAOOBX010000159 DAOOBX010000000
VERSION     DAOOBX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57435_length_2120_cov_2.477482"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLY30_02910"
     CDS             complement(<1..446)
                     /locus_tag="PLY30_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPW65087.1"
                     /translation="MSLIVQKYGGSSVANAEKILRVAQRIVRTRKAGNKVVVVVSAMG
                     DMTDDLITLADKINKNPKEREMDMLLATGEQVSIALLAMAIHKLGYEAVSFTGPQVGI
                     ITDTFHSKAHIQDISPGRIRKALSAGKIVIVAGFQGQTLEGEITTLG"
     gene            complement(556..1758)
                     /gene="apgM"
                     /locus_tag="PLY30_02915"
     CDS             complement(556..1758)
                     /gene="apgM"
                     /locus_tag="PLY30_02915"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPW65088.1"
                     /translation="MKYIVVALSGAADHPTDDLGGKTPLEVAKIPHLSFFAKSGKVGT
                     AKVLPDRAPLAPDAGLFGLLGYEGKLYPGRGPLEAANLELMLEDNEVAFCMNFITESA
                     GLLADATAGNIGTKEAKALINFLNKKVSSDFVRFFSGTAHRHIAVIKDAHGFEALSAR
                     SNDPENVLGQKIEHVLPKGPGEELLKKLMFDARLLLQDHEINQVRVDLGENPANMIWL
                     WGQGKKPALKPIRELYDLTGGAMVASREYAKGLGRMAGLTVMELKEDGEDPAVFYDRI
                     SKVALDALEEKDFICLHLHQPDEASRAGDLKAKVSAIEAIDYFILSKLRKYCERQKES
                     RLLVTPCHATLWKMRSMVSDHAPFVIFGKNVMADEVERFSEVAATTSELRIQKNTELM
                     PFFITKAS"
     gene            complement(1755..>2120)
                     /locus_tag="PLY30_02920"
     CDS             complement(1755..>2120)
                     /locus_tag="PLY30_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPW65089.1"
                     /translation="PVANPETLATAIRIGNPASWQSAVAARDESGGLIDMVTDEEITK
                     AYRFIAEREGVFAEPASAAGVAGVIKLAQKNFFKEGTRLVITLTGSGLKDPDFALSFE
                     DPVYTIEADLDAVREAVQV"
BASE COUNT          397 a          660 c          600 g          463 t
ORIGIN      
        1 ccgagggtcg tgatctcccc ttccagggtc tggccctgga accccgcaac gatcacaatc
       61 ttccccgccg aaagggcctt cctgatgcgt cccgggctga tgtcctggat gtgggccttc
      121 gagtgaaatg tgtcggtgat gatgccaacc tgcgggcccg tgaaggacac cgcctcgtac
      181 ccgagcttgt ggatggccat cgcgaggagc gcgatcgaga cctgctcgcc cgtcgcgagc
      241 aacatgtcca tctcacgttc tttgggattc ttgttgatct tgtccgcaag cgtgatcagg
      301 tcgtccgtca tatcgcccat cgcggacacg accacgacga ccttgttgcc ggcctttcgg
      361 gtccggacga tcctttgggc gacgcgcagg atcttttccg cgttggcgac cgaagagccg
      421 ccatactttt gcacgatcag tgacatgttt ttcctttact gtgtttaaat gttcaacaaa
      481 agcgcaacgc tcgaccgtca actctcagcg tgacatcctg gggtcgattc gttgcgcgtg
      541 ggatgttgcg cgtccttaag aagctttcgt gatgaagaac ggcatgagct ccgtattctt
      601 ctggatcctg agctccgacg ttgtggcggc gacctcggag aaccgctcca cctcgtccgc
      661 catgacattc ttgccgaaaa tgacgaatgg cgcgtgatca ctcaccatcg acctcatttt
      721 ccagagtgtg gcatggcacg gcgtgaccag cagtcgtgat tccttctgcc gttcgcagta
      781 tttccggagc ttggagagga tgaaatagtc gatggcctcg atcgccgaga ccttggcctt
      841 caggtcgccc gcgcgactcg cctcatccgg ctgatgcaga tggaggcaga tgaaatcctt
      901 ttcctcgagc gcgtccagcg cgactttgga gatcctgtca taaaagaccg cggggtcctc
      961 accgtcttcc tttaattcca tgaccgtcag gccggccatc cggccgagcc ccttcgcgta
     1021 ctcgcgggag gccaccatgg cgccgccggt cagatcataa agttcgcgga tcggtttgag
     1081 cgccggtttt ttcccctggc cccacagcca gatcatgttg gccggattct cgccgaggtc
     1141 gacgcggacc tggttgatct cgtggtcctg cagcaggagc cgggcatcga acatgagttt
     1201 cttgaggagc tcttcgccgg ggcctttcgg caacacatgt tcgatctttt ggccgaggac
     1261 gttctccggg tcgttcgacc gcgcggaaag agcctcgaac ccgtgcgcgt ccttaatcac
     1321 cgcgatatgc cggtgggccg tcccggaaaa gaaacggacg aaatcactcg agaccttctt
     1381 gttgaggaaa ttgatgaggg ccttcgcctc tttcgtcccg atgtttcccg cagtggcgtc
     1441 cgccagcaga ccggccgact ccgtgatgaa gttcatacag aacgcgactt cattatcctc
     1501 aagcataagc tcaaggttcg cggcttcaag cggcccgcgc ccggggtaaa gtttcccttc
     1561 atagccaaga agtccgaaga ggccggcatc gggggcaagc ggcgcgcggt ccggaaggac
     1621 tttcgcggtc ccgactttcc cggacttcgc aaaaaaggaa aggtgcggga tcttcgccac
     1681 ttcaagcggc gtcttcccgc ccaaatcatc cgtcggatga tccgcggcgc ccgaaagcgc
     1741 cacaacgata tacttcatac ctgaacagcc tccctgaccg catcaagatc ggcctctatc
     1801 gtatagacag ggtcctcaaa actcaaggcg aaatcggggt ccttgagccc cgagcctgtc
     1861 agcgtgatca cgagccgggt cccttccttg aagaaattct tttgcgcaag cttgattacg
     1921 cccgccacgc cggccgcgga cgccggctcc gcaaaaacgc cttcgcgttc cgcgatgaac
     1981 cggtacgcct ttgtgatctc ctcgtccgtc accatatcga tcaggcctcc cgattcgtcg
     2041 cgagcggcga cggcggactg ccagctcgcg gggttcccga tacggatggc cgtcgcgagc
     2101 gtttccggat tcgcaaccgg
//
LOCUS       DAOOBX010000160         2104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_58031_length_2104_cov_2.993167, whole genome shotgun sequence.
ACCESSION   DAOOBX010000160 DAOOBX010000000
VERSION     DAOOBX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58031_length_2104_cov_2.993167"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLY30_02925"
     CDS             <1..378
                     /locus_tag="PLY30_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65090.1"
                     /translation="VDLSYANRPELQVEAAKLQATRLGERVRWGEFLPKAFLTWEAGA
                     LGEAWTDDIGEVYGAGYKDDPVLKREWRLMLELNWNVGGNKVSYTYDKDKKAPSITQY
                     AAGQGTSLRKNNLTIGILDGLDA"
     gene            379..741
                     /locus_tag="PLY30_02930"
     CDS             379..741
                     /locus_tag="PLY30_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW65091.1"
                     /translation="MNVKQAEVDKLNQVVELEKAEKQVLQDVKQAFYDYQKALIQVRS
                     NTKRAKWRERLRDLARHRLERKEVEISEYMQAESDLVRERGDLHKALKDYYSAKAALN
                     HAVGIQEYLTLEGNHGPR"
     gene            728..>2104
                     /locus_tag="PLY30_02935"
     CDS             728..>2104
                     /locus_tag="PLY30_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW65092.1"
                     /translation="MDPDKTPDYRPGQDPHDKERLRSDPEKLKRLIEKFSHPDHEEPK
                     APEPSPVPPAPEPAPKPVAKPAAPSPSSRIQPPLAPKSFVAPKTVKEPHGPWILRNAP
                     KAVIALVVLLALFIGGKAIFGLFHKTAKVSLKDTMRQQAEAAKDAPVAVKGFKVGRFN
                     YEDSLNVLGTIKGAMEVKLSFEIPGVVSSINYREGERYEEGALLISLKQDDILLRLKR
                     AQAAFNKAESQAKVAENEVTENEKLLKMGAIPASTLEKKKLELDAAKYEAETQRLEMK
                     ANESMLEKSNLYAPSSGMLGGLNIQEGENISQSTLVGTHINTEYVYAEFGIVERDVQK
                     ISVGQKAKVFVDAYPDKSFDGVIENVAPQISGTSRTSTARVRIENPEGLLMPGMFARV
                     RILLYSKKDALVVPTDAVQGGEGEKWLYVIKLNESERPAVKPEEVLPQGGLTGLAAMA
                     LKKKPAAAT"
BASE COUNT          510 a          561 c          658 g          375 t
ORIGIN      
        1 gtggatcttt cctacgcgaa ccgcccggaa ctccaggttg aagccgcaaa actccaggcg
       61 acccggcttg gcgaacgggt ccgctggggg gagttccttc cgaaggcttt tttgacttgg
      121 gaagccggag cgcttggtga agcgtggacg gatgatatcg gggaagttta cggcgcaggc
      181 tacaaggatg atccggttct caaacgtgaa tggcgtctga tgctggagct gaactggaac
      241 gttgggggta acaaggtcag ttatacttac gataaggaca agaaggcgcc gtcgatcacc
      301 cagtacgccg cggggcaggg aacgagcctc cggaagaaca atcttacgat cggcatcctg
      361 gacggcctcg acgcttaagt gaacgtcaaa caggcagagg tcgacaagct gaaccaggtc
      421 gtggaacttg aaaaggccga aaagcaggtc ctgcaggacg tgaagcaggc gttttatgat
      481 tatcagaagg ccctgatcca ggtgcggtcg aataccaaac gcgcgaagtg gagggaacgc
      541 ctccgtgatc ttgcgaggca ccggcttgag cgcaaagagg tcgaaatttc cgagtacatg
      601 caggccgaat ccgatcttgt ccgggagcgg ggggatctgc ataaggccct taaggattat
      661 tactccgcga aagcggctct caaccatgcc gtcggtattc aggaatacct gacgctcgaa
      721 ggaaaccatg gacccagata aaacgcccga ttatcgtccc ggtcaggacc ctcacgacaa
      781 ggagcgactg aggtcggatc ctgaaaaact gaaacgtttg atcgagaagt tctcgcaccc
      841 ggatcacgaa gagccgaagg ctcccgagcc ttcgcccgtt cccccggcac cggaacccgc
      901 gcccaaaccg gttgcgaaac cggcggcgcc gtctccgtcc tcccggattc agccgccgct
      961 tgccccgaaa agctttgtgg cgccgaagac cgtgaaagag cctcacggcc cgtggatatt
     1021 gcggaacgcg ccgaaggctg tgatcgcgct cgtggtcctt ctggcgcttt ttatcggcgg
     1081 taaggcgatc ttcggtctct tccacaagac cgccaaggtt tccctcaaag acacgatgag
     1141 acagcaggcg gaagccgcga aggacgcgcc ggtcgcggtg aaaggattca aggtggggcg
     1201 gttcaattat gaagactccc tcaacgttct cgggacgatc aagggagcga tggaagtgaa
     1261 gctgagcttc gagatacccg gagtcgtcag ctcgatcaac tacagggaag gcgaacgcta
     1321 cgaggaaggc gcgctcctga tctcgctgaa acaggatgat atcctgcttc gtctaaaacg
     1381 cgcgcaggcg gcctttaaca aggccgagtc acaggccaag gttgccgaga atgaggtgac
     1441 agagaacgag aagcttctga agatgggcgc cattcccgcg tcgacgcttg agaagaagaa
     1501 gctggaactg gacgccgcga agtacgaagc ggagacccag cgtctcgaga tgaaggcgaa
     1561 cgaatcaatg ctcgagaaat cgaaccttta cgccccttcc tcgggaatgc tgggagggtt
     1621 gaacatccag gaaggcgaaa acatctccca aagcaccctg gtcggtacgc acatcaacac
     1681 cgaatatgtc tacgcggagt tcgggattgt ggaacgcgat gtgcagaaga tttcggtcgg
     1741 acagaaggcg aaggtcttcg tggacgcgta tcccgacaaa tcctttgatg gtgtgattga
     1801 aaatgtcgcc ccgcagattt cgggaacgag ccggacgtcg acggcgcgtg tccggatcga
     1861 gaaccccgaa gggttgctca tgcccggtat gttcgcccgg gttcgtatcc tgctatacag
     1921 caagaaggac gcactggttg tcccgaccga tgctgtccag ggcggggagg gggagaaatg
     1981 gctttatgtg atcaagctga acgagtctga gcgaccggcc gtgaagcccg aagaagtcct
     2041 tccccagggc gggctgaccg ggcttgcggc gatggcgctc aaaaagaaac ccgcggccgc
     2101 gaca
//
LOCUS       DAOOBX010000161         2088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_58656_length_2088_cov_2.669946, whole genome shotgun sequence.
ACCESSION   DAOOBX010000161 DAOOBX010000000
VERSION     DAOOBX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58656_length_2088_cov_2.669946"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /gene="pyrG"
                     /locus_tag="PLY30_02940"
     CDS             complement(<1..419)
                     /gene="pyrG"
                     /locus_tag="PLY30_02940"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922188.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HPW65093.1"
                     /translation="MPKHIFVTGGVVSSLGKGIASASIAKILEAKGLKVAMQKLDPYL
                     NVDPGTMSPCQHGEVYVTDDGAETDLDLGHYERYTHSTYGRENNLTSGQVYFKVITKE
                     RRGDYLGGTVQVVPHITDTIKEGIRGVSKNKRYDVVIT"
     gene            complement(440..1201)
                     /gene="kdsB"
                     /locus_tag="PLY30_02945"
     CDS             complement(440..1201)
                     /gene="kdsB"
                     /locus_tag="PLY30_02945"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPW65094.1"
                     /translation="MKAIGVIPARLGSTRLPEKILRTIAGKPMIQHVWENARKARSLE
                     DVIVACDDERIERCVRGFGGMAVMTRRDHPNGTSRIAEVMRKFDHEIVINIQGDQPLV
                     DPKALDEMVALFRVSRAPEMLTLALRMTDRAAFANPNVVKIVCDEGGCALYFTRSPVP
                     YAQGQQDREFSFLKHLGVYGYRRGFLLEFVAWKPGVLEMTEKLEQLRVLERGRKIRVI
                     ETDFDFISVDTEEDLREVESRLARARSQGGSAEDV"
     gene            complement(1207..>2088)
                     /gene="rfaE1"
                     /locus_tag="PLY30_02950"
     CDS             complement(1207..>2088)
                     /gene="rfaE1"
                     /locus_tag="PLY30_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HPW65095.1"
                     /translation="ISPEAPVPVVDVKRESYMPGGSLNVAHNIRSLSGKAYPCGVVGR
                     DLYGRMLLKAIRQQGIETGGIVYDPTRPTTLKTRVIAHSQQMVRFDRERTGDVSRSNL
                     DKILKFVKQKIREIDVVILEDYGKGVMQPFLLREVVKLCKKLKKPVLVDPKEKHFDYY
                     TGVTCITPNRKEAYGGYERMVGYRRKMPDLEQVGWALIKKLRLGSVLITLGEDGMALF
                     ERGGKITKVPTAAREVYDVSGAGDTVIATLALSLAAGARMQEAAKIANMAAGIVVGKL
                     GTATASPAELREAIRQA"
BASE COUNT          398 a          663 c          585 g          442 t
ORIGIN      
        1 gtgatcacga cgtcatagcg cttgttcttc gagaccccgc ggattccctc cttgatcgta
       61 tcggtgatat gcgggacaac ctgcacggtg ccgccgaggt aatcgccgcg acgctctttc
      121 gtgatgacct tgaaatagac ctgcccgctc gtgaggttgt tctcgcgtcc gtaggtcgag
      181 tgcgtgtagc gctcgtagtg gccgaggtcg aggtcggtct cggcgccgtc atccgtcaca
      241 tagacctcgc cgtgctggca cgggctcatc gtgcccggat ccacattcag gtacgggtca
      301 agcttctgca tcgcgacctt taggcctttc gcctccagga tcttcgcgat cgaagccgag
      361 gcgatcccct tgccgagcga agacaccaca ccgcctgtca caaaaatatg tttcggcata
      421 tcaccattcc ctcacggggt tatacatcct ccgcggagcc cccctggctg cgggcccgtg
      481 ccagacgcga ctcaacctcc cggaggtcct cttccgtatc cacgctgatg aaatcaaaat
      541 cggtctcgat cacgcgaatc ttccggcccc tttcgaggac cctgagctgc tccagtttct
      601 cggtcatttc caggacaccg ggtttccacg cgacgaattc cagaaggaac ccgcggcggt
      661 atccgtaaac accgaggtgc ttcaaaaagc tgaactcgcg gtcctgctgc ccctgcgcgt
      721 acggaaccgg ggaccgcgta aaataaagcg cgcatccgcc ttcatcgcag acgatcttga
      781 cgacattcgg gttggcgaac gcggcgcggt ccgtcatgcg gagggcgagc gtcagcattt
      841 cgggggcgcg cgagacccgg aaaagcgcca ccatttcatc gagggctttc gggtccacaa
      901 gcggctggtc gccctggata ttaatcacga tctcgtgatc gaattttcgc atgacctccg
      961 cgatacgcga agttccgttc gggtgatcac gtcgggtcat gacggccatg ccgccgaacc
     1021 cccggacaca gcgctcgatc cgttcgtcgt cgcaggccac gatcacatct tcaagcgacc
     1081 gggccttgcg ggcattctcc cagacgtgct ggatcatggg tttgccggcg atcgtccgga
     1141 gaatcttttc gggaaggcgc gtcgaaccaa gacgcgcggg aatgacaccg atcgctttca
     1201 tcgcattcat gcctggcgga tcgcttccct gagctccgcg ggggaggccg ttgccgttcc
     1261 gagctttccg accacaatgc cggccgccat attggcaatc ttcgcggcct cctgcatcct
     1321 ggcacccgcc gcgagcgaga gcgcgagcgt tgcgatcacc gtgtcaccgg caccggacac
     1381 atcgtaaacc tcacgggccg cggtcggaac cttcgtgatc ttgccgcccc tctcgaaaag
     1441 ggccataccg tcctcgccaa gcgtgatcag aacggacccg agcctgagct tttttataag
     1501 cgcccagccg acctgttcaa ggtccggcat tttacgccgg tatccgacca tcctttcata
     1561 tcccccgtac gcttccttgc ggttcggagt gatacaggtc acgccggtat aataatcgaa
     1621 atgtttctcc ttggggtcca ccaagacggg ctttttgagc tttttgcaga gtttcacgac
     1681 ttcgcgcagg agaaagggtt gcatgacgcc cttgccgtaa tcttcgagga tcacgacatc
     1741 gatctcccgg atcttctgct tcacaaattt aagaatcttg tcgagattcg aacgcgacac
     1801 atccccggtc ctttcgcggt cgaagcggac catttgctgg gagtgcgcga tcacacgcgt
     1861 tttgagcgtc gtgggccggg tggggtcata aacgatgccg ccggtctcga tcccctgctg
     1921 gcgtatggcc ttgagcaaca tccgcccgta cagatcacga ccgaccaccc cgcagggata
     1981 cgccttcccc gaaagggacc tgatgttgtg agcgacattc agcgaccctc cgggcatgta
     2041 ggattcgcgc ttaacgtcaa cgacaggaac gggggcctcg ggagaaat
//
LOCUS       DAOOBX010000162         2079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_58999_length_2079_cov_3.485672, whole genome shotgun sequence.
ACCESSION   DAOOBX010000162 DAOOBX010000000
VERSION     DAOOBX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58999_length_2079_cov_3.485672"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLY30_02955"
     CDS             <1..648
                     /locus_tag="PLY30_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPW65096.1"
                     /translation="INITTIEDPVEYQITGVNQVQVKPDIGLTFASGLRAFLRQDPDV
                     ILVGEVRDLETAEICIRASLTGHLVLSTLHTNDAVTAVTRLIDIGVQPYLVSGSIALV
                     AAQRLIRLLCEDCKEAYRPTDKERSDYGIRAETIYRAKGCSKCRNIGYWGRAAIYEVI
                     PVDEDIRRLIVKDANLDAMRRLQKEKKYETLFQNGLKKVERGVTTIEEILSVAYE"
     gene            641..1981
                     /locus_tag="PLY30_02960"
     CDS             641..1981
                     /locus_tag="PLY30_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPW65097.1"
                     /translation="MNNPQAGMPPRGGDHPERYECRVRLRDNPNELKHVTIEASSIDE
                     CVYRLLGQGYLVVAVKQPGHAEFESAAKLVTAGAARPAPAPKKRARKTSSIVLGKGVS
                     TRELIFFAVQLSTLLKAGIPLIRSLEIVRRGTPNPHFQNIIEKITKSVSGGSSFGHAI
                     RERKGVFPWVWVNLVEVGESTGKLPECLEEIAQYQEASARIQSKVVTAFFYPSILTVA
                     VIGALTFLLIYIVPKFSAIFEQQKMELPALTQAVVAASDLVRHHAFLLLLIPVPFIIL
                     GFLIKRSPEIRFFMHMMFLRVPFFGEMSIQIAVVRFARGMSTLLKSGVQILQALDIGA
                     RLVENAYLEAGIKQVAQGVRGGQGLGAQLEARRVFPVFMTQLLSVGEETGQLEKFLDL
                     IGTYYEERVDAFLTRLTTMLEPMMLVVMGVVIGTIVVSMFLPIIELSTRGGMGG"
BASE COUNT          411 a          617 c          621 g          430 t
ORIGIN      
        1 atcaacatca ccacgattga ggaccctgtt gaataccaga ttacgggcgt aaaccaggtc
       61 caggtgaagc cggatatcgg gctgaccttt gcctcgggcc tgcgggcgtt tcttcgccag
      121 gacccggacg tcattctggt cggcgaggta cgcgaccttg agacggctga gatttgtatc
      181 cgcgcgtcgc tgaccggtca cctcgtcctc tcgacccttc acacgaacga tgccgtgacc
      241 gcggtcaccc ggttgatcga tatcggggtg cagccgtatc ttgtgtcggg cagtatcgcg
      301 cttgttgcgg cccagcggct gatccgtctc ctctgcgagg attgcaagga agcctaccgg
      361 cccaccgaca aggagcgttc cgattacggg atccgggccg aaaccattta ccgggccaaa
      421 ggctgttcga aatgccgcaa tatcggctac tggggtcgcg ccgcgatcta cgaggttatc
      481 cctgttgatg aggacatccg gcgtctcatt gtcaaggacg cgaacctcga cgccatgcgc
      541 aggctgcaaa aggaaaaaaa atacgagacc cttttccaga acggcctcaa gaaggtggaa
      601 cggggcgtca ctacgatcga ggagattctg agcgttgcct atgaataacc cgcaagccgg
      661 catgccccct cgcggcggag atcacccgga gcggtacgaa tgccgggtgc gtctgaggga
      721 taatccgaac gaactgaaac atgtcacgat cgaggcctcg tcgatcgacg aatgcgtgta
      781 ccgtctgctc ggccagggct atctcgtggt cgcggtgaaa cagcccggcc atgcggaatt
      841 cgaaagcgcc gccaagctcg tgacggccgg cgccgcgcgt cccgcgcccg cgccgaaaaa
      901 gcgcgcgcgc aagacgtcct cgattgtcct cgggaaaggc gtcagcaccc gcgagctgat
      961 cttcttcgcc gttcagctct cgacgctcct caaggccggc attccgctca tccgttccct
     1021 cgagatcgtc cggcgcggaa ctccgaaccc tcatttccag aatatcatcg aaaagatcac
     1081 gaagtcggtt tcgggcggaa gctcgttcgg ccacgccatc cgcgagcgca agggggtttt
     1141 tccgtgggtg tgggtgaacc tcgtggaggt cggcgaatcg accgggaaac ttcccgaatg
     1201 cctcgaggag atcgcccaat accaggaggc ctcggcccgc atccagtcga aggtcgtcac
     1261 ggcgtttttc tatccgtcga tcctgaccgt cgcggtgatc ggcgccctga cgttcctgct
     1321 gatctacatc gtgccgaagt tcagcgccat tttcgaacag cagaaaatgg agcttcccgc
     1381 gctgacgcag gccgtggtcg ccgcgagcga tctcgttcgg catcatgcct ttctcctgct
     1441 cctcatcccg gttccgttca tcatactcgg gttcctcata aaacgctcgc ccgagatccg
     1501 gtttttcatg cacatgatgt tccttcgcgt tccgttcttc ggggagatgt cgatccagat
     1561 cgcggtggtc cgttttgcgc gcggcatgag cacgctcctc aaatcgggcg tccagatcct
     1621 ccaggcactt gacatcggcg cccggctcgt cgaaaatgcg tatcttgagg ccggtatcaa
     1681 acaggtcgcg cagggcgtgc gcggcggcca ggggctcggc gcccagctcg aggcgcggcg
     1741 ggtcttcccg gtcttcatga cgcagctttt atcggtcgga gaggagacgg gacagctcga
     1801 gaagttcctc gacctcatcg ggacctatta tgaagagcgg gttgacgctt ttttgacccg
     1861 gttgacgacg atgctggagc ccatgatgct ggtggtcatg ggcgtcgtga tcgggacaat
     1921 cgtcgtttcc atgttcctcc cgatcatcga actctcgaca cgcggcggga tgggcggtta
     1981 agtaataggg aaaacgcggc gcaaggtttt acgggtgcac tgttaaaaac aaaggagaaa
     2041 ttccatgatg aataaaaaag gtttcacgct cattgaggt
//
LOCUS       DAOOBX010000163         2051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_60086_length_2051_cov_2.686874, whole genome shotgun sequence.
ACCESSION   DAOOBX010000163 DAOOBX010000000
VERSION     DAOOBX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60086_length_2051_cov_2.686874"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="PLY30_02965"
     CDS             complement(<1..1459)
                     /locus_tag="PLY30_02965"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPW65098.1"
                     /translation="MLSCPVVHAVTGSKAAVVTPDPRATKAALKVLNEGGNAMDALLT
                     AQAVLNVVAPQSSGIGGGASLLYYDRATRAVLYFDGSVRSPAKLFPKMFLDKGGRPLS
                     ADAVRTSGLSVGVPGALKLLAEVQARYGSGKKNFGALLAPAITFAEEGMEVSEPLGAA
                     LKQNMSVLGTSRSFREIFFRNGLTAGAGDRIRQTDLAETLKLLKKHGPDIFYEGKIGA
                     AVVKAAGAAPAPQSVLTAGDLKNYAIVKQDPLYVSYGEQDLFTAGPPSAGGVMLFASL
                     NLLNELGLSAFPGKPEAVHLLAETMKLTGGLDVAIGDPDFFDLPVGKILSKEWTGERA
                     SRVALNKAMRRSAFTRSSAGTRNTLSVSILIIDRYGNIVNYSASMGNSFGSGILVPGY
                     GFFLNDLMLDFDTDEAAMKDPDFPNIPGPRQRPRNFFTPLFVFREGRPVMALSASDGR
                     ESAKTVLNLFVQRADFNVPCAGILEVSRVFDRDGVM"
     gene            complement(1563..>2051)
                     /locus_tag="PLY30_02970"
     CDS             complement(1563..>2051)
                     /locus_tag="PLY30_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPW65099.1"
                     /translation="REMAKHNGAVYMRTGRPKAAVVHQASTQFRIGKGVRLREGKDLT
                     IVANGFEVVEALDAAERLAKEGIRASVIDMHTVKPLDKELLLEEAKKTGRILVAEEHQ
                     IWGGLGSAVAAFLSANHPCKMGFVAIQDTFAESGKSEELLEKYGLTAPYIVAAAKKIL
                     SK"
BASE COUNT          446 a          634 c          584 g          387 t
ORIGIN      
        1 ccataacacc atcccggtca aaaacacggg acacctcgag aatccccgcg caggggacat
       61 tgaaatccgc gcgctggaca aaaagattga gcaccgtctt cgccgactcc cggccgtcgg
      121 acgcgctgag agccataaca ggacggcctt cccggaaaac aaaaaggggt gtaaaaaaat
      181 tcctgggtcg ctgacggggc cccgggatgt tcgggaaatc ggggtctttc atcgcggctt
      241 cgtccgtgtc gaaatccagc atgagatcgt tcagaaaaaa accgtatccg gggaccagga
      301 tcccgctgcc gaaagaattt cccatggaag cggaataatt cacgatattg ccgtaccggt
      361 cgataatcag gatggagacc gaaagggtat tcctcgttcc ggctgaagac ctcgtaaatg
      421 cggaacggcg catggccttg ttcagggcca cgcgggacgc gcgttccccg gtccattctt
      481 tcgaaaggat cttcccgaca ggaaggtcga aaaaatcggg atctccgatc gccacgtcca
      541 gcccccccgt gagtttcatt gtctctgcca gaagatgaac ggcctcgggc tttcccggaa
      601 aagcgctgag cccgagctcg ttcagaagat tgagagacgc gaacagcatg accccgcccg
      661 cggacggcgg gcctgccgta aaaaggtcct gctcgccgta cgagacatag agggggtcct
      721 gtttcacgat cgcgtagttt ttcagatcgc ccgcggtcag gacggattgg ggagcgggcg
      781 cggccccggc agccttgact accgcggcgc cgatcttccc ttcatagaaa atatcgggac
      841 cgtgtttctt caagagcttc agcgtttcgg caagatcggt ctgacgaatc cggtcccccg
      901 cccccgcggt cagaccgttc cggaaaaaga tctccctgaa agatctcgag gtcccgagca
      961 cggacatgtt ctgcttcagg gccgcgccga gaggctccga aacttccatc ccttcttcgg
     1021 cgaacgtgat cgccggcgcg aggagggcgc caaagttctt tttgccgctc ccgtaacggg
     1081 cctggacctc cgcgagcaat ttcaaagcgc ccggcacccc caccgaaagc ccgctcgttc
     1141 tgaccgcgtc cgcggaaaga ggcctgcctc ccttgtccag gaacattttc ggaaaaagtt
     1201 tcgccggcga gcggacgctt ccgtcaaaat aaaggaccgc ccgcgttgcg cgatcataat
     1261 aaagaagcga cgctccaccc ccgatgccgc tggattgggg cgcgacaacg ttcagaacgg
     1321 cctgcgcggt gagaagcgcg tccatcgcat tcccgccttc gttcaggact ttgagggccg
     1381 ctttggttgc gcgcgggtcg ggcgtcacca ctgcggcctt ggaccccgtc acggcgtgga
     1441 caacagggca ggacaacatc aagcaagaca ggacgggaag gaaggaaacg agaaatgaaa
     1501 aacggccgcg ccaaaacccc cggaggtgat ccgccgcacc ccgcacgaca cgcgccgcgg
     1561 tactattttg agaggatctt cttggcagcc gcaacaatgt agggagccgt caggccgtat
     1621 ttttcgagaa gttcttcgga cttgccggat tcggcaaaag tgtcctggat tgcgacaaaa
     1681 cccatcttgc aggggtgatt cgcggaaagg aacgcggcca cggcgcttcc aagcccgccc
     1741 cagatctgat gttcttccgc cacaagaatc cttccggttt ttttcgcctc ttcgagcaaa
     1801 agctccttgt cgagaggttt cacggtatgc atatcgatca cggacgcccg gataccttcc
     1861 ttcgcgagcc tttcggccgc gtcaagagct tccaccactt caaaaccgtt cgccacaatc
     1921 gtcaggtctt tcccctcgcg aagacgcacg ccctttccga tccggaactg cgtggaggcc
     1981 tggtggacga ccgccgcttt cggacgaccg gtcctcatgt agacggcgcc gttatgcttc
     2041 gccatttcgc g
//
LOCUS       DAOOBX010000164         2050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_60125_length_2050_cov_3.883709, whole genome shotgun sequence.
ACCESSION   DAOOBX010000164 DAOOBX010000000
VERSION     DAOOBX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60125_length_2050_cov_3.883709"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLY30_02975"
     CDS             <1..235
                     /locus_tag="PLY30_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="starch synthase"
                     /protein_id="HPW65100.1"
                     /translation="PFDAESGKGNGFVFEDYTVEALSDAVAGAVTVFRQDKPWRTLVK
                     NAMESDFSWSSTAKKYSKIYESVKRRHFKISKV"
     gene            275..1345
                     /gene="leuB"
                     /locus_tag="PLY30_02980"
     CDS             275..1345
                     /gene="leuB"
                     /locus_tag="PLY30_02980"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPW65101.1"
                     /translation="MYKIAVFPGDGIGVEVTAQAMKVLRKVEPKTGLRFETEELLAGG
                     ASIDQFGVPLTPETLAKAQKADAVFLGAVGGPKWDSLPSDKRPEKGLLGLRKGLNAYA
                     NVRPVKMFPQLASRSPIREEIVEGLDFIILRELVSGVYFGSPRGIEKLPDGTERGFNT
                     MSYTTPEIARIARRAFEFARKRRKKVTSVDKANVLDVSQLWRKVVIEVANGFPDVELS
                     HRYVDDCAMQLIRDPKQFDVIVTSNLFGDILSDESAMLTGSIGMLPSASVGDKVAMYE
                     PVHGSAPDIAGKDIANPLATILSVAMMMEHSFDQPKAARAIEAAVEKVLADGYRTADI
                     YAEGTTRVGCVKMGDLVTERIS"
     gene            1553..>2050
                     /locus_tag="PLY30_02985"
     CDS             1553..>2050
                     /locus_tag="PLY30_02985"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPW65102.1"
                     /translation="MKKPDIAILGVTGAVGQELLAILEERKFPFASLKLLASGRSAGK
                     EQKFMGKTYKIEEATPDSFEGIDLVFASAGGSISKALAPYAVKAGAVVVDNTSAFRMD
                     PEVPLVIPEINPEDIAKHKGIIANPNCSTIIALVPLWPIHKAVKIKRFVAVTYQAASG
                     AGAKAM"
BASE COUNT          455 a          544 c          641 g          410 t
ORIGIN      
        1 gccgtttgac gcggagtccg gtaagggaaa cgggtttgtt ttcgaggatt acacggtcga
       61 ggctctctcg gacgcggtcg ccggggcggt cacggttttc cggcaggata agccgtggag
      121 aacactcgtg aagaatgcca tggaaagtga cttctcctgg tcctcgaccg cgaagaaata
      181 ttccaaaatc tacgaaagtg tcaaacgcag gcatttcaag atcagcaagg tctgagcgtt
      241 ttaaagagtc cacgctcgaa aggtgaccca tcccatgtac aaaatagcag tatttcccgg
      301 tgacggtatc ggagtcgaag taacggcgca ggccatgaag gtcctgcgga aggtcgagcc
      361 caagaccggt ctcagattcg agaccgagga acttctggcc ggtggtgctt ccatcgatca
      421 atttggcgtc cctctcacgc ccgagaccct tgcgaaggcc cagaaagccg acgcggtttt
      481 tctcggcgcg gtcggcggcc cgaaatggga cagcctgccc tcggataagc gtccggaaaa
      541 aggattgctc gggctccgca aggggcttaa cgcctatgcg aacgtccgtc ccgtgaagat
      601 gttcccgcag ctcgcatccc gttcgccgat cagggaagag atcgtcgagg gcctcgattt
      661 catcatcctg cgagagctcg tgagcggcgt ttatttcggt tcgcctcgcg ggatcgaaaa
      721 acttcccgac ggcacggagc ggggtttcaa tacgatgagc tacacgacgc ctgaaatcgc
      781 gcggatcgcg cggagggcct tcgaattcgc ccggaagcgg cggaaaaaag tgacttcggt
      841 tgacaaggcc aatgtcctcg acgtgagtca gctctggcgc aaggtggtga tcgaggttgc
      901 gaatggtttc ccggatgtcg aactctcaca tcgttatgtg gatgattgcg cgatgcagct
      961 gatccgcgac ccgaaacagt tcgacgtgat cgtcacaagc aatctcttcg gggacatcct
     1021 gagcgacgaa tccgcgatgc tcacgggctc gatcgggatg ctgccgtcgg cttcggtcgg
     1081 cgacaaggtg gcgatgtacg aaccggtaca cggttcggcg cccgacatcg cgggcaagga
     1141 catcgcgaat cctctcgcga cgatcctctc ggtcgcgatg atgatggaac acagttttga
     1201 ccagccgaag gccgcgcgcg cgatcgaggc ggcggtcgag aaggtccttg ccgacggcta
     1261 tcgcaccgcg gatatttacg cggaaggcac gaccagggtc ggttgtgtga aaatgggtga
     1321 tcttgtaacg gaacgtatat cgtagggggc gggcgaagtt ttataaaaga acagagccat
     1381 ttcttgtcga tcgcccgcgg attccttcgt gaaatggaag ctcaatgtcg aatcagcgtc
     1441 gatttgggtt tcggtgaaag cagaatcgct gaataactga taaaggaggc ggggagcgtg
     1501 ccgtggcgga tgctgaatcc cgagcgccaa acgctctacg ctgtgcgtta atatgaaaaa
     1561 acccgatatt gcaattctcg gcgtcaccgg cgctgtcggt caggaacttt tggccatcct
     1621 tgaggaacga aagttcccgt tcgcgtcgct caaactgctc gcgtccggtc gttcggcggg
     1681 gaaagagcag aaattcatgg ggaagacgta caagatcgaa gaagcgacac cggattcttt
     1741 cgagggaatc gacctcgtgt tcgcgagtgc gggcggatcg atctcgaaag ccctcgcgcc
     1801 gtacgccgtg aaggcgggcg cggtggtcgt ggataacaca agcgcgttcc ggatggatcc
     1861 ggaggttccg cttgtgatcc ctgagatcaa ccccgaggac attgcgaagc acaaagggat
     1921 catcgcgaac ccgaactgct ccacgatcat cgcacttgtt ccgctttggc cgatccacaa
     1981 ggccgtgaag atcaagcgct ttgtcgcagt gacctatcag gccgcgagcg gtgccggcgc
     2041 gaaggcgatg
//
LOCUS       DAOOBX010000165         2048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_60214_length_2048_cov_3.278976, whole genome shotgun sequence.
ACCESSION   DAOOBX010000165 DAOOBX010000000
VERSION     DAOOBX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60214_length_2048_cov_3.278976"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..640)
                     /locus_tag="PLY30_02990"
     CDS             complement(11..640)
                     /locus_tag="PLY30_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPW65103.1"
                     /translation="MEWFQDLFARLSDLQGLIRWGGYFIVTAIVFAETGLLAGFFLPG
                     DSLLVTAGLLCAVDRSMNIWVLNLLLCAAAIIGDTTGYWIGYHFGPKIFNREDSFFFH
                     KEHLDRTQKFYEKHGPKTIVLARFVPIVRTFAPTVAGAGCMSYKKFLFYNIAGGIGWV
                     LSMTLIGFFLARSIPDIEKKVHWVILIVIVLSLLPIAKELLHHHKKPGS"
     gene            complement(708..1178)
                     /locus_tag="PLY30_02995"
     CDS             complement(708..1178)
                     /locus_tag="PLY30_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPW65104.1"
                     /translation="MRFVKLVLSLVFCFAAGAAGAAVISQAIPSWYATLEKPSFAPPA
                     RIFGPVWSALYLMMGIAAWIVWSKGLKEEAVRKGLALFLFQLALNSVWSYLFFGLRSP
                     FCGLLDILFLWAAILATIGQFSKVSITAAVLLIPYLAWVTFAAGLNFGICLLNR"
     gene            complement(1293..1928)
                     /locus_tag="PLY30_03000"
     CDS             complement(1293..1928)
                     /locus_tag="PLY30_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65105.1"
                     /translation="MRLIFRRCPLAAALFLMLTFFHPGLTWKKPGTAEAFTHSLKRTN
                     GDNILDASIQVAAAPDCLLRMLFSAYHIRELMKDSADLVEIANEGPGRYELRSVHKNK
                     LCSLDTVYRRDLRKDMRRVTFELIRCEQVSKVAPKLVSSKGYYEVLPDPAGSLLKYHE
                     ETRLEASRWDPRAATLVKLVERDTLLFLKKIKKYSEKTACRDSLPRLTVCP"
BASE COUNT          469 a          581 c          618 g          380 t
ORIGIN      
        1 gcgatcgctc tcatgacccg ggtttcttgt gatgatggag caactctttg gcgatcggga
       61 gcagggaaag aacgatcacg atcaggatga cccaatggac ctttttttcg atgtccggga
      121 tggatctcgc gagaaagaat ccgatcagcg tcatgctcag gacccagccg attccgcccg
      181 cgatattata aaagaggaat ttcttgtaac tcatgcaccc cgcgccggcc acggtcggcg
      241 cgaaggttct cacgatcgga acgaaccggg cgagcacgat cgttttggga ccgtgtttct
      301 cgtagaattt ctgcgttcgg tcgaggtgct ccttgtgaaa gaaaaaggaa tcctccctgt
      361 tgaagatctt tggtccgaaa tgatacccga tccagtagcc ggtggtgtcc ccgatgatcg
      421 cggcggcgca aagcaggagg ttcaggaccc agatgttcat ggatcgatcg accgcgcaca
      481 agagcccggc cgtgacgaga agggaatccc ccggcaggaa aaaccccgcc aaaagcccgg
      541 tttccgcgaa cacgatggcc gtaacgatga aatatccgcc ccagcgaatg aggccctgaa
      601 ggtcggacaa ccttgcgaaa agatcctgaa accattccat agcaacatcc cgattctgaa
      661 atgcggacgt cggaggccgg aacgccccgc cttctgacgg cccgcggtta acggttcaat
      721 aaacagattc cgaagttcag accggcggcg aaggtgaccc acgcgagata cgggatcagg
      781 aggaccgcgg cagtgatcga gaccttcgag aactgtccta ttgtagcgag gattgcggcc
      841 cataggaaca gaatatcgag aagcccgcag aacggcgacc ggagaccgaa aaaaagataa
      901 gaccagaccg agttcagggc gagctggaac aggaagagcg ccaacccctt acggaccgct
      961 tcttccttca ggcccttcga ccagacgatc catgccgcga tccccatcat cagatacaac
     1021 gcgctccaga ccgggccgaa gatccgggcg ggcggcgcga aggaaggttt ttccagggtc
     1081 gcgtaccagg acgggatcgc ctgcgagatg acggccgcgc cggccgctcc cgccgcgaaa
     1141 cagaatacca gggaaagaac aagcttgacg aatctcatgc gaactccctt accgaaagat
     1201 ggaaatgcct tgccgcgtca cgattttatc aagataccgt cggactttga agatgagttc
     1261 gcgccggaaa cggcgccgga gccgggcaac cttcacgggc agacggttaa gcgcggaagg
     1321 gaatcgcggc aggcggtctt ttcggaatat tttttgatct tcttcaggaa aagaagcgtg
     1381 tcgcgttcga cgagcttgac aagcgtcgcc gcgcgggggt cccaccggga cgcttcgagc
     1441 cgggtctcct catgatattt caggagggaa ccggccgggt ccggcaagac ctcgtagtac
     1501 cctttggaac tgacgagctt cggcgcgacc ttgctcacct gttcgcagcg gatcagttcg
     1561 aatgtcacac gccgcatgtc cttccggagg tcacggcgat aaacggtgtc gaggctgcag
     1621 agcttgttct tgtggaccga acggagttca tagcgccccg ggccctcgtt cgcgatctcc
     1681 acgagatccg cgctgtcctt catcagttcc cggatgtgat aggcgctgaa gagcatccgg
     1741 agcaggcagt ccggcgcggc cgcgacctgg atgctcgcgt cgaggatatt gtccccgttt
     1801 gtccgcttga ggctgtgcgt gaatgcctcg gcggtccccg gttttttcca ggtcagtcca
     1861 ggatgaaaga acgtgagcat caaaaaaagc gcggcggcaa gagggcatcg tctgaagatg
     1921 agtctcatga tcagcgggcc ctcccgagcg cttgctcgag ttcgtccgga agcgcggcct
     1981 cgatatccag gacctcattc cgttccgggt gccggatgcg gaaatgccac gcgtgaagga
     2041 acagccgg
//
LOCUS       DAOOBX010000166         2039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_60575_length_2039_cov_3.434476, whole genome shotgun sequence.
ACCESSION   DAOOBX010000166 DAOOBX010000000
VERSION     DAOOBX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60575_length_2039_cov_3.434476"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PLY30_03005"
     CDS             complement(<1..1053)
                     /locus_tag="PLY30_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPW65106.1"
                     /translation="MRLNSLLGEILIGKKMITVQQLSQALVEQKETREPLGQVLIRFG
                     FITEANLRVALSEQLGISFVSPDELVPRIDQRLKEVIPPDFARKHLVLPLSRDERSLK
                     IAVGKMPDLIFLDNLRKMTGLDILTVLSTEADIKRAIDLYYSVTDLQEVVSSSEVDAA
                     SQQYIETKDESRVDLESAMAEAGQAPVVRLVDMMIKKSLDDRASDIHIEPFYDKLTVR
                     YRIDGTLYELPPPPRELHNAVVSRIKIISKLDIAEKRVPQDGSFSLSYKARRIDVRVS
                     TVPTVFGEKVVMRILDKRTDLLDLRALGFEEEQLRHFELALTRPYGLIFVTGPTGSGK
                     STTLYAALNKIHSPDIN"
     gene            complement(1105..>2039)
                     /locus_tag="PLY30_03010"
     CDS             complement(1105..>2039)
                     /locus_tag="PLY30_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HPW65107.1"
                     /translation="DFFSNVGNTLKDAANQYDPRIESGTITTSGTRDFRNFTLTIQAL
                     ENANKIKVIAKPKILVLDNHAALIKISTNAAIGVTTFTTSGAAAGTTQESIERAEVGT
                     ILRVTPLVNQRNQITLTVEPTFATVAESTIAVSASETGDTTVRTARTTLMVNDGQTIA
                     LGGMLFSNQTKNDRKVPFFGNIPVVGKAFFTNSGKSIEDRELILFLTPYIIRDPSELR
                     TPDVPDKRLKFEDEKAPFYDVKKKEWYRELKDGKEKPIDYESYFDVRKRLMQATLDTL
                     DGKVSTPASQAAIPAVPSYEEDISGIPAVQEIPN"
BASE COUNT          381 a          578 c          619 g          461 t
ORIGIN      
        1 gttgatgtcg ggcgaatgaa tcttgttgag ggcagcgtac agcgtggtgc ttttgccgct
       61 ccccgtcggg ccggtcacaa agatcagtcc gtacggccgc gtgagcgcga gctcgaaatg
      121 ccggagctgt tcctcctcga aaccgagcgc ccgcaggtcc agaagatcgg tgcgtttgtc
      181 caggatacgc atgaccactt tttcaccaaa cacggtcggg accgtcgaaa cgcggacatc
      241 gatacggcgc gccttgtagg aaagggaaaa cgaaccgtcc tgcgggaccc gcttttcggc
      301 gatgtccagc ttggagatga tcttgatacg ggacacgacc gcgttgtgga gctcgcgggg
      361 cggcggcggc agttcataaa gggtaccgtc gatgcggtaa cggaccgtca gcttgtcgta
      421 gaacggctcg atgtggatat cgctcgcgcg gtcgtcgagg gattttttga tcatcatgtc
      481 cacgagccgc acgacggggg cctgtcccgc ttcggccatc gcggattcga gatcgacccg
      541 cgattcgtcc ttggtctcga tatactgctg gctcgcggca tccacctcgg acgaggagac
      601 gacttcctgg agatcggtca cgctgtaata caggtcaatc gcgcgcttga tatcggcttc
      661 cgtcgagagc acagtgagga tgtcaagccc cgtcattttc cggaggttat cgaggaagat
      721 caggtccggc atcttcccga ccgcgatctt cagcgaccgc tcgtcgcggg aaagcggaag
      781 gaccaggtgt ttgcgggcaa aatccggcgg gatgacctcc ttcaggcgct ggtcgatacg
      841 cggcaccagc tcatcgggag aaacaaagga gatgccgagc tgttcggaaa gcgccacgcg
      901 aagattcgcc tcggtgatga aaccaaaccg gatcaggacc tggccgagag gttcacgcgt
      961 ttctttctgc tcgacgaggg cctgcgaaag ctgctgcacg gtgatcattt ttttaccgat
     1021 caggatctcg cccagcaaac tgttgagtct catattcctc cgttccggct tccggaaacg
     1081 ccggtcactt gccttggcct gtctctaatt cggaatctcc tgaaccgccg ggatccccga
     1141 aatatcttcc tcataggaag gtacggcggg tatcgcggcc tgggacgccg gcgttgacac
     1201 tttgccgtcc agcgtatcaa gcgtcgcctg catcaaccgt ttccggacgt cgaaatagct
     1261 ttcataatca atgggttttt ccttcccgtc cttgagttcc cggtaccatt ccttcttttt
     1321 cacatcatag aagggcgcct tttcatcctc aaatttcagg cgtttgtccg ggacatcggg
     1381 agttctgagc tccgacggat cgcggataat gtagggcgtc aggaaaagga tcagttcgcg
     1441 gtcttcgatc gatttgccgc tattcgtgaa aaaggccttc ccgacgacgg ggatgttccc
     1501 gaagaacggc actttccggt cgttcttggt ctgattgctg aagagcatcc cgccaagcgc
     1561 gatggtctgg ccgtcattca ccatcagggt cgtccgcgcc gtgcggaccg tcgtgtcacc
     1621 cgtctcggag gcgctcaccg cgatagttga ctcggccacc gtcgcgaacg tcggctccac
     1681 ggtcagggtg atctggttac gctggttgac gagcggcgtc acgcggagga tcgtgccgac
     1741 ctcggcacgc tcgatacttt cctgcgtcgt gccggccgcg gcgccggagg tcgtgaaggt
     1801 cgtcacgccg atcgcggcgt tggtggagat cttgattagc gcggcgtggt tgtcgaggac
     1861 cagaatcttc ggtttcgcga taaccttgat cttgttcgcg ttttccagag cctggatcgt
     1921 cagcgtaaaa ttccggaaat cgcgggtacc gctggtcgtg atggtccccg attcaatgcg
     1981 ggggtcatac tggttcgcgg cgtccttgag ggtgttcccg acattgctga agaaatccc
//
LOCUS       DAOOBX010000167         2024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_61185_length_2024_cov_2.158964, whole genome shotgun sequence.
ACCESSION   DAOOBX010000167 DAOOBX010000000
VERSION     DAOOBX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61185_length_2024_cov_2.158964"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..807
                     /gene="rpe"
                     /locus_tag="PLY30_03015"
     CDS             154..807
                     /gene="rpe"
                     /locus_tag="PLY30_03015"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPW65108.1"
                     /translation="MKKIMLAPSILSADFTRLGDEVREVETGGCDVLHVDVMDGQFVP
                     NLTIGPLVVSALRKVTKLPLDVHLMIDRPLRYIAEFRKAGADWITIHVEAEPDVKKCL
                     SEIRSSGAKAGISIKPITPVSVIEPYLNDLDLVLVMSVEPGFGGQAFMPDQMSKISAL
                     RRQFKGLISVDGGIDPVTAPQAIAAGADVLVAGSAIFGKPDRGQAIARFRDIIRKYS"
     gene            807..>2024
                     /gene="lepA"
                     /locus_tag="PLY30_03020"
     CDS             807..>2024
                     /gene="lepA"
                     /locus_tag="PLY30_03020"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPW65109.1"
                     /translation="MNPSQIRNFSIIAHIDHGKSTLADRLLLHAGAITKREFRDQILD
                     DMELERERGITIKARAVRIHYKNHWLNLIDTPGHIDFTYEVSKSLAACEGALLLVDAG
                     QGIQAQTVTNLYLAMERNLTIIPVVTKIDLPTAEPEKVKKEVAAFLGVDEKTVLLASA
                     KTGEGAAEILDAIIQRVPAPKGDSAQPLKALIFDSIYDTYQGVITYFRVMDGKLRAHD
                     QVLMMQTKTQQEALEIGIFTPTPQPVDELGPGSVGYMTANIKEVAEVKIGDTLTLAAE
                     PAGNPLPGYQDVKPMVFCGLYPINAKDFSLLRDALAKLRLNDSSFAYEPETSASLGFG
                     YRCGFLGLLHMDIIKERLEREFRLELIITAPSVVYQVFTKKGEIIELDNPTKLPHPTM
                     IETIDEPYIRAHVM"
BASE COUNT          445 a          561 c          603 g          415 t
ORIGIN      
        1 agagaatatt ctgcagaaga tcgaggaagt cacggaagat gaaatccggg aggttgcgag
       61 gtcgatcttc cgcacgaacg ggttgaacat ggccttggtc ggtcccgtta acgaaaaaat
      121 acagcgtgag atccggaaag ggcttgtgat cgaatgaaaa aaatcatgct cgctccaagt
      181 atcctgtcgg ccgattttac aaggctcggc gatgaggtcc gggaagtcga aaccggcggc
      241 tgtgatgtcc tgcacgtgga tgtcatggat ggtcagttcg tgccgaacct tacgatcggt
      301 cccctggttg tcagcgccct gcggaaggtg acgaagctgc cgctggatgt ccatctgatg
      361 attgaccggc cgttgcgcta cattgcggaa ttccggaagg ccggcgcgga ttggatcacg
      421 atccacgtcg aggcggaacc ggacgtcaaa aaatgtcttt ccgaaatccg gagttccggc
      481 gcgaaagcgg ggatttcgat caagccgatc acgccggtct cggtgatcga gccgtatctc
      541 aatgaccttg accttgtact cgtgatgtcg gtcgagcccg gttttggcgg acaggcgttc
      601 atgccggatc agatgtccaa gatctcggcg cttcgccggc aattcaaggg ccttatttca
      661 gtggatggcg ggatcgatcc cgtgacggct ccgcaggcca tcgcggccgg cgcggacgtg
      721 ctcgtggccg gtagcgcgat cttcggaaaa cccgaccgcg ggcaggcgat tgcccggttc
      781 cgggacatca ttcgaaaata ttcctgatga acccctccca gatccggaat ttttccatta
      841 tcgcgcatat cgatcacggg aaatccacgc tcgcggaccg tcttctgctg cacgcgggcg
      901 cgattacgaa acgtgaattc cgcgaccaga ttctcgatga tatggagctt gagcgcgagc
      961 gcgggatcac gatcaaggcg cgcgcggtcc gcatccacta caagaaccat tggctcaacc
     1021 tgatcgacac gcccgggcat atcgatttta cttatgaggt ttcaaagagc cttgccgcct
     1081 gcgaaggcgc ccttcttctc gtcgacgcgg ggcagggaat ccaggcgcag accgtcacga
     1141 atctttatct tgcgatggag cgaaacctta cgatcatccc ggtcgtgacg aagatcgacc
     1201 tcccgacggc ggaacccgag aaggtcaaaa aagaagtggc ggcgttcctc ggggtcgatg
     1261 aaaagacggt tcttctcgcg agcgccaaaa cgggtgaggg agccgcggaa atcctggacg
     1321 cgatcatcca gcgcgttccg gcgccgaaag gggattccgc gcagccgctc aaggcgctga
     1381 tctttgattc catctacgat acctatcagg gcgtgatcac atacttccgc gtaatggacg
     1441 gcaagttgcg ggcgcacgac caggtgttga tgatgcagac gaagacgcag caggaagcgc
     1501 ttgagatcgg gattttcacc ccaacccccc agccggtcga cgagctcggc cccggctctg
     1561 tcggttatat gaccgcgaat atcaaggagg tcgcagaggt caagatcggg gacaccttga
     1621 ctctcgcggc cgagcccgcc gggaatccgc tccccgggta tcaggatgtg aaaccgatgg
     1681 tgttctgcgg tctctatccg atcaacgcga aggatttctc gcttttgcgt gatgcgctcg
     1741 ccaagctccg cctgaatgat tcctcgttcg cctacgaacc cgaaacatcg gcttcgctcg
     1801 gattcggtta ccgttgcggg ttccttggat tgctgcacat ggatatcatc aaggaacggc
     1861 tcgagcgcga attcaggctt gaattgatta tcacggcgcc gagcgtggtc tatcaggtct
     1921 tcacgaagaa aggggagatc atcgagctcg acaatccgac gaaactgccg catccgacga
     1981 tgatcgaaac gatcgacgaa ccctatatcc gggcccatgt gatg
//
LOCUS       DAOOBX010000168         2019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_61391_length_2019_cov_2.757128, whole genome shotgun sequence.
ACCESSION   DAOOBX010000168 DAOOBX010000000
VERSION     DAOOBX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61391_length_2019_cov_2.757128"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /gene="bioD"
                     /locus_tag="PLY30_03025"
     CDS             complement(<1..657)
                     /gene="bioD"
                     /locus_tag="PLY30_03025"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HPW65110.1"
                     /translation="MKAGIFITGTDTEVGKTIVSAGLALCLRDRGIKVGVMKPVATGC
                     YGAEERLISHDAAFLMEAAEGQFPPLHSPARFRNPLSPNVAAMLEKKEVNVDRILKSF
                     RDLGKHYDYMIVEGIGGIMVPLKKDYYVANLIRDMKLPVIVVSHAGLGAINHALLTVD
                     AAMIRGLEIKGIIFNRVAVSNYSLAELTNPKVIHDLSGVPILGSLPDIDDLQVESCSF
                     G"
     gene            complement(746..>2019)
                     /gene="bioA"
                     /locus_tag="PLY30_03030"
     CDS             complement(746..>2019)
                     /gene="bioA"
                     /locus_tag="PLY30_03030"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HPW65111.1"
                     /translation="KGAGCYLIDVDGRRYLDGVSSLWCNVFGHRVPAIDKAVKRQLGK
                     IAHTTFLGLSHPAAIELSEKLIAIAPKPSPGSGDRPLTRVFYSDSGSESVEIALKMAY
                     QYWQQRGQPKKKKFVKLTNAYHGDTIGSVSVGGIDLFHQIYRPLLFETLEVPSPYRYR
                     WPASDDAEQVKQESLEMMEKVLRENAEEVAALVMEPLMQGAAGMIDQPAGYISRAREL
                     TRKYNTLLIFDEVATGFGRTGKLFAAEHEGVIPDIMTVAKGLTGGYLPLAATLTTERV
                     YEAFLGQYEDFKTFFHGHTYTANPLACAAALATLELFKKDRTIEKLQPKIALLRKRLQ
                     DFYKLDAVGDIRQAGTMIGIELVRDKRTRAEFAPKEKIGVRVIELARKKGAILRPLGP
                     VIVLMPPLAMTEKELNRLLDIAYESIKEGTE"
BASE COUNT          400 a          624 c          542 g          453 t
ORIGIN      
        1 gccgaaactg cagctttcga cctgcagatc gtcaatatcg ggaaggctcc caaggatcgg
       61 aacacccgag aggtcgtgga tcactttcgg attcgtcagt tcagccaggg aataattgct
      121 gacggcgacc cggttgaaaa taatgccttt gatctccagc ccgcggatca tggcggcatc
      181 gaccgtcagc agcgcgtgat tgatcgcccc gagaccggcg tggctcacaa cgatcaccgg
      241 cagtttcatg tcgcggatca ggttcgcgac atagtaatcc tttttcagcg ggaccatgat
      301 tccgccgatt ccctccacga tcatgtaatc gtagtgtttg ccgagatcgc ggaaactttt
      361 gaggatgcga tcgacgttca cttccttctt ttcgagcatc gcggcaacgt tcggtgaaag
      421 cgggttccgg aaacgcgcgg ggctgtgaag cggcgggaac tggccttcgg ccgcctccat
      481 caggaatgcc gcgtcgtggg agatgagcct ttcttccgcg ccgtaacagc cggtcgcgac
      541 cggcttcatc accccgacct tgatgccgcg atcgcggaga cagagcgcaa gccccgccga
      601 aacaatggtc ttcccgactt ctgtatccgt gcctgtgatg aaaataccgg ccttcatgag
      661 accctcgcgc cacgggccgc ggacaacgca caacagggaa gttttccgag tacgcgctgt
      721 gtgttgtgcg ttttgcaggc aataatcatt ccgttccttc ctttatagac tcatacgcga
      781 tatcaagcaa tctgttcagt tctttttccg tcatcgcaag cggaggcatc aggacaatca
      841 ccggaccgag cggccgcagg atcgcgcctt tttttcttgc gagctcgatc acgcgcaccc
      901 cgatcttctc tttcggcgcg aactccgccc ttgttcgttt gtcccgaacc aattcaatcc
      961 cgatcatggt tccggcctgc cggatatccc ccaccgcgtc gagcttgtaa aaatcctgta
     1021 atcttttccg caaaagcgcg atcttcggct gaagtttctc gatcgtccgg tcttttttga
     1081 aaagctcgag cgtcgcgagc gccgccgcgc acgcaagcgg gttcgccgtg tacgtgtgcc
     1141 cgtggaaaaa ggtcttgaag tcctcgtatt gcccgagaaa cgcttcataa acccgttctg
     1201 ttgttagggt tgcggcgagc ggaagatagc ctcccgtaag ccccttggcc acggtcatga
     1261 tgtcggggat cacgccttcg tgttcagccg caaacagctt ccccgttcgc ccgaaacccg
     1321 tcgcgacctc gtcaaagatc aagagcgtat tatactttcg cgtcagctcc ctcgcacggg
     1381 aaatgtatcc cgcgggctga tcgatcatcc ccgcggcacc ctgcatgagc ggttccatca
     1441 caagcgcggc aacttcctcc gcgttctcgc gcagaacctt ttccatcatc tcgagggatt
     1501 cttgcttgac ctgttccgcg tcatcgctcg cgggccaccg gtagcggtac ggagagggaa
     1561 cctccagggt ctcgaagaga agcggccggt agatttgatg aaaaagatcg atcccgccca
     1621 cgctcacgga cccgatcgtg tcgccatgat aagcgttcgt aagtttgacg aacttctttt
     1681 ttttcggttg tcctcgctgc tgccagtact gatacgccat tttcagcgcg atctcgaccg
     1741 attcggaacc ggaatccgag tagaacaccc gcgtcagcgg acggtcaccg ctcccgggcg
     1801 acggtttcgg ggcgatcgcg atcagctttt ccgacagctc gatcgccgcc ggatgtgaga
     1861 gaccgagaaa cgtcgtgtgg gcaatctttc cgagctgcct cttgacggcc ttgtcgatcg
     1921 cagggacccg gtgcccgaac acattgcacc agagcgacga cacgccgtcc agataccgcc
     1981 gcccatccac atcgatcaga tagcagcccg ctccctttt
//
LOCUS       DAOOBX010000169         2017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_61452_length_2017_cov_5.045362, whole genome shotgun sequence.
ACCESSION   DAOOBX010000169 DAOOBX010000000
VERSION     DAOOBX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61452_length_2017_cov_5.045362"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2017)
                     /locus_tag="PLY30_03035"
     CDS             complement(<1..>2017)
                     /locus_tag="PLY30_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65112.1"
                     /translation="SQRVYVYDMDSVSPNGLYRYALYTWNDKDQPALLEAGYFSVSPD
                     GAVVRSAESVVRYSSEVLSSVGAPPAVFDEHLAFASATDALMARLGVASSDIAQTRIL
                     LDPAGSGPLTVVLSSGGVDYHYSFDTVTGEVLLLRTRSAGSASGTYLWTSYDAEGRKI
                     SESLEAADGTVTDLFTYRYATAGELAVIDHSNRTVKVLRLNADGTTGETLRAGFYDDA
                     NRIYSLQGAGAAEANWYTYGADGVMLTSDDAPTSRPKRGYDRPSGGPGRRMPGGDNTS
                     LEEKIRRLRAMMRDFQECSEENMRSDWKLAKKCEDMMGSIGGLQKDIENEQYRGMGLG
                     GRGGEGGGDQDLNYHNPAATPAYVAPLEGVGSAPLANPAAVVVDNTDGTKTITLGGFV
                     SVWKAGETGWGTGNDVLLETTETVTGTTWVYENGLVTRAVTRSGGAETLLARYTFDLA
                     ARTVTVSEADGSSCLYRFTDEKDPFGTGELIRAEVTRAGVVLVETYESGRLMSSCAAG
                     GASCTNYAYDTLSNTVTLTASDGATRIVNMGFDGRLGTGDDYLVGGTDMLDVSMEDGR
                     LLSVLDGVNNSFTQFFYEGNRVNIVTTLAGLEISNITVDLGADGVLGTADDRYVVVDA
                     YMSVPVLDSDGRYLREDGTVSLSPAEAAVTVVHLVTLYDWQGVSSEAS"
BASE COUNT          424 a          696 c          559 g          338 t
ORIGIN      
        1 gacgcttcgg aagacacgcc ctgccagtcg tagagcgtca ccagatgaac caccgtcacc
       61 gccgcttcgg cgggcgacaa ggacaccgtt ccgtcctccc tgagataccg accgtccgag
      121 tccagaaccg gaacggacat ataggcatcg acgacgacat aacggtcatc cgccgtgccg
      181 agcacgccgt ctgcgccgag gtccaccgta atgttcgaaa tctcgagacc cgcgagggtc
      241 gtcacgatat tgacccggtt cccctcataa aagaactgcg tgaaagagtt attcaccccg
      301 tccagcaccg aaagaagcct gccgtcttcc atactcacat ccagcatatc cgtcccgccc
      361 acgagataat cgtcccccgt gccgagacga ccgtcaaaac ccatgttcac gatccgcgtc
      421 gcgccatccg aagccgtgag cgttaccgtg tttgaaaggg tgtcatacgc ataattcgtg
      481 cagcttgcgc cgcccgcggc gcaggaggac atgagccgac ccgactcata cgtctcaacc
      541 agcaccacgc cggcccgggt gacctccgcc cggatcagtt ccccggtacc gaacgggtcc
      601 ttctcgtccg taaacctgta taaacaggaa gaaccgtccg cttcggaaac cgtcacggtc
      661 ctggccgcaa gatcgaacgt gtaacgcgca agcagcgtct ccgcaccccc ggagcgcgtc
      721 accgcccggg tcacgagacc gttctcataa acccatgtcg tccccgtcac ggtctccgtc
      781 gtctcgagaa gaacgtcatt ccccgtgccc cagcccgttt ccccggcctt ccacaccgac
      841 acaaacccgc ccagggtaat cgtcttcgtg ccgtccgtgt tatccaccac gaccgcggcc
      901 ggattggcaa gaggagcgct gcctacccct tcgagcggcg caacatacgc cggggtcgcg
      961 gcagggttgt gatagttcag gtcctggtcg ccgccaccct cgccgccgcg gccgccgagg
     1021 cccatcccgc gatactgctc gttctcgata tccttctgca ggcccccgat cgaacccatc
     1081 atgtcttcgc acttcttcgc gagcttccag tccgagcgca tgttctcctc cgaacactcc
     1141 tggaaatcac gcatcatcgc ccgaagacgg cggatcttct cttccagact cgtgttgtcg
     1201 cctccaggca tccgacgacc gggaccgccc gacggccggt cgtaaccgcg cttcggacgg
     1261 gatgtcgggg catcatcgga ggtcagcatc actccatccg cgccgtatgt gtaccagttc
     1321 gcctccgcgg cccccgcgcc ctgcagcgaa taaatcctgt tcgcgtcatc ataaaaaccc
     1381 gcgcgcaacg tctcacccgt cgtgccgtcc gcgttcaggc gcagaacctt caccgtccgg
     1441 ttcgagtgat cgatcaccgc gagctcgccg gctgtcgcgt agcgatacgt gaaaagatcc
     1501 gtcacggtgc cgtcggcagc ctcgaggctc tccgaaatct tccggccttc cgcgtcatac
     1561 gacgtccaaa ggtaagtccc ggacgcggaa ccggcggaac gggtcctcaa aagcaggacc
     1621 tcgcccgtca cggtatcgaa cgaataatga taatccacac cgccggagga gagcaccacg
     1681 gtcagcggac ccgaccccgc gggatcgaga aggatgcgcg tctgcgcgat atcggacgag
     1741 gcgacgccaa ggcgcgccat aagcgcgtcg gtggcggaag cgaaagcaag gtgctcgtca
     1801 aacacggcgg gaggagcgcc gacggaagag agaacttcgg aggagtagcg gacgacggat
     1861 tcggcgctgc ggacaacggc gccatcggga gaaacggaaa aataaccagc ctcaagaagc
     1921 gccggctgat ccttgtcatt ccaggtgtaa agggcgtaac ggtaaagacc gttcggggaa
     1981 acgctgtcca tgtcgtagac atagactcgc tgcgacg
//
LOCUS       DAOOBX010000170         2008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_61862_length_2008_cov_2.800307, whole genome shotgun sequence.
ACCESSION   DAOOBX010000170 DAOOBX010000000
VERSION     DAOOBX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61862_length_2008_cov_2.800307"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLY30_03040"
     CDS             <1..335
                     /locus_tag="PLY30_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HPW65113.1"
                     /translation="IILDFLKTSKASLGSSKSLVDLGAEGEAVLSFISHLNQVGQTGA
                     TPRHPKGLATCCHGEEPHVVGWRFISERRAINTDANCGWAQGKADVLYVADAFAVMRK
                     VNELLAKA"
     gene            361..993
                     /locus_tag="PLY30_03045"
     CDS             361..993
                     /locus_tag="PLY30_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPW65114.1"
                     /translation="MVFGFLLPKDGNFFDLLDKQAGYATEAATRFAKIVADFKVDDEE
                     VERMKEIEHRGDEVSHQIMDQLNKTFITPFDREDIHALANELDDIIDILYAITKRLRV
                     YKVLSADRNLTEFARVIEMSVKGVAQAVQGLRDIKNQKDIINACIEVNSLENLGDKMR
                     DDMLGELFEKYAKDPIAVIKWKEIYQDAETVLDICEDAVHVVESILVKQA"
     gene            1065..>2008
                     /locus_tag="PLY30_03050"
     CDS             1065..>2008
                     /locus_tag="PLY30_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPW65115.1"
                     /translation="MTTYIVFLIFLALFFDFLNGFHDSANSIATIVSTRVLTPQKAVI
                     WATSFNLAAFFFFGHAVAKTMGKGIVDIAIVDNNIIFGTLMGACIWNIITWYFGLPTS
                     SSHALIGGLVGAALVKTGPSALVWKGILKTVAFIFISPAVGLILGVGFGILVYWFFRK
                     SSPLQVDHLFRKGQLLSAALYSLGHGGNDAQKTMGIIASLLFSSGLLGKEFHIPIWVG
                     LACYLALGLGTMFGGWRIVKTMGQKVAKLRPVDGFCAESGAAMTLFVSTALGVPVSTT
                     HTITGAIMGIGSLKRLSAVKWGVAGDIVWAWILTIPCSA"
BASE COUNT          432 a          546 c          578 g          452 t
ORIGIN      
        1 agatcatcct cgacttcctc aagacgagca aggcctcgct cggaagcagt aaatcccttg
       61 tggacctcgg cgcggaaggg gaagcggtgc tttccttcat cagccacctg aaccaggtcg
      121 gccagaccgg cgcgacgccg cgtcacccga aggggctcgc gacctgctgt cacggcgagg
      181 aaccgcacgt ggttggatgg cggttcatca gcgagcgacg ggcgatcaac acagacgcga
      241 actgcggttg ggcgcagggc aaggcggacg tgctctatgt cgcagacgcg tttgcggtca
      301 tgcgaaaggt caacgaattg ctggcaaaag cttaaggttt tatcagcaaa ggagaaaatc
      361 atggtgttcg gtttcctgct ccccaaagac ggaaattttt tcgatctcct cgataagcag
      421 gccggttacg ccacggaggc cgcgactcgc ttcgcgaaga tcgtcgcgga tttcaaggtg
      481 gacgatgagg aggtcgaaag aatgaaagaa attgaacacc gcggcgatga agtctcccac
      541 cagatcatgg accagctcaa taaaaccttc atcacgccgt tcgatcgcga ggatattcac
      601 gcgctcgcca atgagcttga tgacatcatc gatattctct acgcgatcac gaagcgcttg
      661 cgtgtctaca aggtcctttc cgcggaccga aaccttacgg aattcgctcg cgtgatcgag
      721 atgtcggtca aaggcgtggc gcaggccgtc cagggcctcc gggacataaa gaaccagaag
      781 gatatcatca atgcctgtat cgaggtgaac agcctcgaga atctcggcga caagatgcgc
      841 gatgacatgc ttggcgagct cttcgaaaaa tatgccaagg atcccatcgc cgtgatcaaa
      901 tggaaagaga tttaccagga tgccgaaacg gtcctcgaca tttgcgagga cgcggtgcat
      961 gtggtcgaat cgatcctggt caaacaggcc taaaccatga tgtccaggtt tcattggtga
     1021 atggacagtc atgatccccg gcttgtttta attaaaggaa aatcatgacg acttacatcg
     1081 tctttctcat ttttctcgcg ctcttttttg attttctgaa tgggttccat gattccgcga
     1141 attcgatcgc gacgatcgtt tccacacgcg tgctgacccc tcaaaaagcg gtgatctggg
     1201 cgacatcgtt taaccttgcc gcttttttct tttttggaca cgccgttgcg aaaacgatgg
     1261 gaaaggggat cgttgacatt gcgatcgtgg acaataacat catcttcgga accctgatgg
     1321 gagcatgtat ctggaacatc ataacctggt atttcgggct cccgacgagc tcctcccatg
     1381 ccctgatcgg ggggctcgtt ggcgccgctc tcgtgaagac cggcccgagc gccctcgtct
     1441 ggaagggcat cctcaaaacg gttgctttca tttttatctc acccgccgtg ggcctgatcc
     1501 tgggggtcgg cttcggaatc ctcgtctact ggtttttccg gaaaagctct ccgctgcagg
     1561 tcgaccatct tttccggaaa gggcagcttc tttcggcggc gctctatagc ctggggcacg
     1621 gtggcaacga cgcgcagaag acgatgggga tcatcgcgag ccttcttttc agctcgggcc
     1681 ttctcggcaa ggagttccat atcccgatct gggtcggact cgcttgctat ctcgcgctcg
     1741 gtctcgggac gatgttcggc ggctggagga tcgtaaagac gatggggcag aaggtcgcga
     1801 agctccgtcc ggtggacggg ttctgtgcgg aatccggcgc tgccatgacc ctttttgtct
     1861 caacagccct cggtgtgccc gtcagcacga cccatacgat cacgggggcc atcatgggca
     1921 tcggatcgct caaacgactg agcgccgtga agtggggtgt cgcgggcgat atcgtctggg
     1981 cctggatcct gactatcccg tgctcggc
//
LOCUS       DAOOBX010000171         1993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_62516_length_1993_cov_2.772962, whole genome shotgun sequence.
ACCESSION   DAOOBX010000171 DAOOBX010000000
VERSION     DAOOBX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62516_length_1993_cov_2.772962"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLY30_03055"
     CDS             <1..670
                     /locus_tag="PLY30_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65116.1"
                     /translation="TLTTYRLGEFVLDPVDIKYKAGEGAEQTIQTNKIYLTVKSVAEG
                     EPKEDIRDVKTVVPLTKHTGKLLIIPAILLALLAACLIYRNRTAKGGAALATETVLTS
                     GEEALARLAELFDSDLLKRGFVKLYYLKLSEILRVYLEKRYGILAVESTTYEIMRAFR
                     DIPLTTELKEKLNYVLSSADLAKFAKWVPQPAEVLSINKKAEEIVRESTPAETPEGEA
                     PRGV"
     gene            660..1640
                     /locus_tag="PLY30_03060"
     CDS             660..1640
                     /locus_tag="PLY30_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPW65117.1"
                     /translation="MEFRSPVFFLLLLLVPVVFAWAKRRNRLELAYSSLKFVQGRPVT
                     LRMALYQLLPLLRGLILTLFVIALARPATASSNKEYQTKGVDIVIALDISGSMLAEDF
                     QPENRIFVAKQEAIKFIQGRENDRIGLVVFSKKAFTQCPLTTDYRILTELLKEIRVGM
                     IADGTAIGLGIATSVNRLRDSDAKSKVIILITDGSNNAGNIDPVTAAELAKTFGIKIY
                     TIAVGKGGLVPFPVDDPLFGKRYVQANVEIDEMILKRIADITGGLFFRARDPKSLSGI
                     YEKINQLEKSEVKVKEYKSYHELFPLFLIPALMLLVFDIAVRQTVLLKVP"
     gene            1723..>1993
                     /locus_tag="PLY30_03065"
     CDS             1723..>1993
                     /locus_tag="PLY30_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65118.1"
                     /translation="MQFFAPRSFMLLWLVPVVIALYWLAHALWKKRMATLICERTLVP
                     KLLPGYRASEFVTRALLMALIILLSVLALARPQWGEEKKQIQRKGV"
BASE COUNT          426 a          630 c          540 g          397 t
ORIGIN      
        1 tacactcacg acctaccgac tcggagaatt cgttctcgat cccgtggaca tcaaatataa
       61 ggcgggggag ggggccgaac aaacaatcca gacgaacaag atctacctca ccgtcaagag
      121 cgtcgcggaa ggggagccca aggaggacat ccgggacgtc aagactgtcg tccccctcac
      181 aaagcacacc ggaaaactgc tgatcatccc ggctatcctg ctggcccttc ttgccgcctg
      241 tctcatttac cggaatcgca cggcgaaggg gggcgcggct ctcgcgaccg aaacggtcct
      301 gacctccggg gaagaagcgc tcgcgcggct cgcggagctt ttcgattccg atctcctcaa
      361 acgcgggttc gtgaaactct actatctgaa gctttccgag atcttgcgcg tctatctcga
      421 aaagcgctac ggcatcctcg ccgtcgaatc cacgacctat gagatcatga gagcgttccg
      481 cgatatcccg ctgacaacgg aactgaaaga aaaactgaat tacgtgctga gttcggccga
      541 cctcgcgaaa ttcgccaagt gggtcccgca gcccgccgag gtactttcga tcaacaaaaa
      601 agccgaagag atcgtccggg aatcaacacc cgcggaaacg ccggaagggg aggcgccccg
      661 tggagtttag gagccccgtt ttcttcctgt tactgctcct tgtgccggtc gttttcgcgt
      721 gggccaagcg tcgtaacagg cttgagctgg cctattcttc gctcaagttc gttcagggcc
      781 ggcccgtcac gctccgcatg gccctgtatc agctcttacc gctcctgcgg gggctgatcc
      841 tgaccctctt tgtgatcgcc ctggcccggc ccgcaaccgc gtcgagcaac aaggaatacc
      901 agacaaaggg cgtcgacatc gtgatcgcgc tcgacatctc cggctcaatg ctcgccgagg
      961 acttccagcc cgaaaaccgc atcttcgtcg cgaaacagga ggccatcaag ttcatccagg
     1021 gccgcgagaa tgatcggatc ggccttgtgg tcttttccaa gaaggccttc acgcaatgtc
     1081 cgctgacgac cgattaccgc atcctgaccg aactcctgaa ggagatccgc gtcggcatga
     1141 tcgcggacgg cacagcgatc ggcctcggca tcgcgacctc ggtgaaccgc ctccgcgact
     1201 cggacgcgaa aagcaaggtc atcatcctga tcacggacgg ttccaataac gccggcaaca
     1261 tcgaccccgt cacggccgcg gagctcgcga agaccttcgg gatcaagatt tacacgattg
     1321 ccgtcggaaa aggcggcctc gtaccgttcc cggtcgatga cccgctgttc ggaaagcgct
     1381 acgtccaggc caatgtcgag atcgatgaga tgatcctcaa gcggatcgcc gacatcacgg
     1441 gcggcctttt cttccgcgcc cgcgatccca aatccctgtc cggaatctat gagaagatca
     1501 accagctcga aaaatccgag gtcaaggtca aggaatacaa aagctatcat gaactttttc
     1561 cgcttttcct catcccggcg ctgatgctgc ttgtcttcga tatcgcggtc cgccaaaccg
     1621 tgctcctgaa ggtgccctga tggtcaatac catttttacc gaggttcacc aggacaacgc
     1681 cggcttctcg atctgttttg cggcagaaag ccgggtgccc tgatgcagtt cttcgctccc
     1741 cggagtttca tgctcctttg gctcgtgccg gtcgtgatcg cgctctactg gctcgcgcat
     1801 gccctctgga aaaaacggat ggccacgctg atctgcgaac ggacgctggt cccgaagctg
     1861 ctcccgggat accgcgcttc cgaattcgtc acgcgggcgc tcctcatggc tctcatcatc
     1921 cttctgtccg tcctcgcgct cgcgcgcccg caatggggtg aagaaaagaa acagatccag
     1981 cgaaaaggcg tgg
//
LOCUS       DAOOBX010000172         1991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_62602_length_1991_cov_3.366219, whole genome shotgun sequence.
ACCESSION   DAOOBX010000172 DAOOBX010000000
VERSION     DAOOBX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62602_length_1991_cov_3.366219"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLY30_03070"
     CDS             complement(<1..482)
                     /locus_tag="PLY30_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW65119.1"
                     /translation="MPNPLTRCPVLYEDKAVILVHKPSGVLSHPNAKGQTAAYEGPYD
                     PDTREFRTPAGPLWLIHRLDREASGILLAAKTPGAAKACRAAFEQQAVEKDYVVLVAR
                     KPIPAKGRWSDFLAVKRRPGGTRAFVTRGPRANALLQYATKEIYPRMNLALLEIRLIT
                     G"
     gene            complement(875..1147)
                     /locus_tag="PLY30_03075"
     CDS             complement(875..1147)
                     /locus_tag="PLY30_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HPW65120.1"
                     /translation="MDMKIFLATLSAIFFAELADKTQLVTLTMSGKTGKPWSVLLGSL
                     TGYALVALISVVIGAALAKYIRPEYIRYAGATAFVVIGALMFLDKV"
     gene            complement(1310..>1991)
                     /locus_tag="PLY30_03080"
     CDS             complement(1310..>1991)
                     /locus_tag="PLY30_03080"
                     /inference="COORDINATES: protein motif:HMM:NF037962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HPW65121.1"
                     /translation="SALYARRCISLGTLLAVFLSTSDEAIPVILADTDQAHLIFDLLG
                     AKLVIATAAGYLIDLLIKTPLHPGTHPVLHDPAVCVEEHRHCSCHSHFCREPWWKTYL
                     LCPVKHTASVALFIFVVSLIIGLVIAGAGEENLGKLFFSGTPFQPLLAVLVGLIPNCA
                     ASVAIAEVYLKGAITFGSTIAGLCASAGLGILVLFKELKDRRETFRILGLLALVSLAA
                     GLLLNCFS"
BASE COUNT          433 a          541 c          617 g          400 t
ORIGIN      
        1 cccgtgatta gccggatttc gagaagcgcg aggttcatgc ggggataaat ttcctttgtc
       61 gcgtactgaa gcaacgcgtt cgcccgggga ccacgtgtga caaatgccct ggtgccgccc
      121 ggtcgccgtt tcacggcgag aaaatccgac cagcggccct tggccgggat cggcttgcgc
      181 gcgacgagga cgacgtaatc cttctcgacc gcctgttgtt caaatgccgc gcggcaagcc
      241 ttcgccgcgc cgggtgtttt ggccgcgagc aggataccgg atgcctcccg gtcgaggcgg
      301 tgaatcagcc agagcggccc cgccggcgtc cggaactcgc gggtatccgg atcgtagggg
      361 ccctcatagg ccgcggtctg tcctttcgcg tttggatgag acaggacgcc cgacggctta
      421 tggacaagga tcacggcctt gtcttcgtaa aggaccggac atcgggtaag gggattaggc
      481 atcgcgcaga gcaggtcagg gaaggttcga gtgttttgac gaggtatccc gtgaagatga
      541 tgtcgggtgt cgtgatcgtt ccgcaaatcg acaccggaaa attgtcccgg accttttcac
      601 gagagagccg gaagagccgg caggtcagcc agtcgaccac cgctccaacc tacgaggttg
      661 gagtgatcca acctcgtagg ttgatggggt tagcggcagc aggatttctt tttggccttc
      721 ttggcgggtt tttttgctgc ctttttcgcc ttcttcgctt ttttcacttt cttggccata
      781 gttttggctc cttcccttgg ttgaggctca acgcacaagg ctcaacgccc aacgatactt
      841 tcgttgcgcg tcgaacattg tccgttgcgc ggtgttaaac tttgtcgagg aacatgaggg
      901 ccccgatcac gacgaacgcc gtcgcgccgg catagcggat atattccggc cggatatatt
      961 tcgcgagcgc cgcgccgatg accacggaga tcagggcaac caaagcgtat cccgtgagcg
     1021 agccgagcag gacggaccag ggcttcccgg tctttcccga catcgtcaac gtcacgagct
     1081 gcgttttgtc ggcgagctcc gcaaaaaaaa tcgcgctgag cgtcgccaaa aaaatcttca
     1141 tgtccataaa tgatctccga aagacgtttg aacggtctct attgtaaccg aatcggcaaa
     1201 ctcttgaaag aattgagcgc aatttatttt ttcgcggccc ggattcagca aagagaacgg
     1261 tctttcggga agttctcccc gcgcggatcc gggaaaaatc cggcggtgtt caggaaaaac
     1321 aattcagaag aagtcccgcg gcgaggctca cgagcgcgag aagtccgagg atgcgaaagg
     1381 tctcgcggcg gtcctttagt tccttgaaaa gaacgagaat tccgaggccg gcgctcgcgc
     1441 aaagtcccgc gatggtcgaa ccgaacgtga tcgctccctt caggtagact tccgcgatcg
     1501 cgaccgaggc ggcacagttc gggatcaggc cgaccaggac ggccaggagc ggctggaagg
     1561 gtgttcccga aaaaaagagc ttcccgaggt tttcctctcc cgccccggcg atcacgagcc
     1621 ctataatcaa cgaaaccacg aaaatgaaca gggccacgct ggccgtgtgt ttaaccggac
     1681 aaagaagata cgtcttccac cagggctcac ggcagaaatg ggaatggcat gagcagtggc
     1741 ggtgttcctc gacacagacg gccggatcat ggaggaccgg gtgcgttccg ggatgaaggg
     1801 gggtcttgat caaaagatcg atcaaatagc cggcggcggt cgcaatgacg agtttggctc
     1861 cgagaaggtc aaagatgaga tgcgcctggt ccgtgtcggc gaggatgacc gggatggctt
     1921 catccgaagt ggagagaaag accgcgagca gggtgccgag cgagatgcac cgccgggcat
     1981 aaagggcgct t
//
LOCUS       DAOOBX010000173         1987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_62789_length_1987_cov_2.653209, whole genome shotgun sequence.
ACCESSION   DAOOBX010000173 DAOOBX010000000
VERSION     DAOOBX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62789_length_1987_cov_2.653209"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLY30_03085"
     CDS             <1..282
                     /locus_tag="PLY30_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW65122.1"
                     /translation="PFPVLMPEAIIYPRKAVKKKWEGRTIVAAEVLPDGSVGRTALAK
                     SSGHEILDAAAEDAIKTWKFGYPPGNNGEAFPRYVDIPVTFRLQSEDGS"
     gene            342..1175
                     /locus_tag="PLY30_03090"
     CDS             342..1175
                     /locus_tag="PLY30_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW65123.1"
                     /translation="MSAPGVSKAGPSRSEPRGSSPQRIVSINLCTDELLLDLVGPERV
                     AAVTKFCGDPKVSTVAARAGGIQKITADIEHVRDRDPDLILGGRFNGRETFRFFRKTG
                     VPVLLFDVPKSFEDIYRDIRRLAEAVGEPEKGDAIIKSMQAELAELNGVSHRFREMVT
                     DPVRAIFFQSGGYVPGAETFENAIMEAAGLSNVALELGIRGYGRMGLEELIKARPDVI
                     IFSSEQRDSKTVRGEVLDHPAIKKALPRATMVTIPTLYLNCGSPASVEAVRILVRETA
                     V"
     gene            1172..>1987
                     /locus_tag="PLY30_03095"
     CDS             1172..>1987
                     /locus_tag="PLY30_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPW65124.1"
                     /translation="MNRSDTRSPRPVSQGADCHTRCGISGDGVPGWLLYSVLGTLLAA
                     AFLGSLSLGTLKYSLWDAIRDPSSDLAFIFWDIRLPRAVLGLLVGATLGLAGAAMQGF
                     LRNPLAEPGILGVTGGAALGAVAVFYTGLVAVFPMMLPVGGIAGAFVSVLLLYLFAGT
                     LASVQTLILAGVAINVIAFAGTSLMLNLVKDPYAVLDIVFWQMGSLADRSFRHVVLIL
                     PFVLAGWGLLLWDGRALEALSLGETTASSLGISLRRLRARVVLGTAIEVGASVA"
BASE COUNT          320 a          554 c          659 g          454 t
ORIGIN      
        1 ccctttcctg ttctgatgcc ggaagcgatc atctatcccc ggaaggctgt taaaaaaaaa
       61 tgggaaggtc ggacgatcgt tgccgcggaa gtgcttccgg acggttccgt ggggcgtacc
      121 gctctggcga aatcctcggg gcatgagatt ctggatgcgg ctgcggaaga cgccatcaaa
      181 acctggaagt ttggatatcc tcccgggaat aacggtgagg cgtttccccg gtatgtggac
      241 attccggtca cattcaggct tcagagtgag gatgggtctt gagttttgta aggaggggtt
      301 cttttttttg cgctttttgt tttgccctct gcgccggggc cttgagtgcg cccggtgttt
      361 caaaagccgg accctcgcgt tccgagcccc ggggttcgag tccccaacgc atcgtttcca
      421 tcaatctctg cacggacgaa ctgctgctcg atctggtggg gccggagcgc gtcgcggcgg
      481 tgacgaaatt ctgcggcgat cccaaggtat cgacggtcgc ggcgcgggcg ggcgggattc
      541 aaaagatcac cgccgatatc gagcatgtcc gggatcgcga ccccgacctg atcctcggag
      601 gcaggttcaa tggcagggaa acgttccgct ttttccggaa aacgggagtc cccgtcctcc
      661 tttttgatgt cccgaagagt ttcgaggaca tttaccggga tattcgccgg cttgcggaag
      721 cggttggaga gccggaaaaa ggtgatgcga tcatcaagtc aatgcaggcc gaattggccg
      781 aacttaatgg ggtcagtcac cgttttaggg aaatggtgac tgaccccgtt cgtgcgatct
      841 tttttcagag cggtggctat gttcccggcg ccgaaacctt cgagaacgcg atcatggaag
      901 ccgcggggct ttcgaatgtc gcgctggaac tcggcatcag gggttatggc agaatggggc
      961 tcgaagagct tataaaggcg aggccggatg tgatcatttt ctcaagcgag cagagggatt
     1021 cgaagaccgt gcgcggcgaa gtgctggacc accccgcgat caaaaaagcc ctgccccgcg
     1081 cgacgatggt gacgatcccg acactatatc tcaactgcgg gtcgccggcc tcggtggagg
     1141 cggtgcggat tctcgtcagg gagacggccg tatgaaccgt tcggataccc ggtcgccacg
     1201 cccggtgtcg cagggcgccg attgccacac ccggtgtggc atttcaggag acggggtgcc
     1261 gggctggctg ctttattccg ttctcgggac gcttcttgcg gccgcgttcc tcggatcgct
     1321 cagcctcggg acgctgaaat attcgctttg ggacgcgatc cgcgacccct cctccgatct
     1381 cgcgttcatt ttctgggaca tccgtttgcc gcgcgccgtg ctcggactcc tcgtcggtgc
     1441 cacgctcggt ctcgcgggcg cggcgatgca gggattcctc cggaatcctc tggcggagcc
     1501 ggggattttg ggtgtgacgg gcggtgccgc gctcggcgcg gtcgcggtct tttacacggg
     1561 gcttgtggcg gtgttcccga tgatgcttcc tgtcggcggc attgccggcg cttttgtttc
     1621 tgtcctgctg ctttatcttt tcgcgggaac ccttgcgagt gtgcagacgc tgatcctggc
     1681 cggcgttgcg atcaacgtga tcgcgttcgc ggggacctcg ttgatgctga atcttgtgaa
     1741 agacccctac gcggtcctcg atatcgtgtt ctggcagatg ggatcgctcg cggaccggag
     1801 tttccggcac gttgtcctga tccttccgtt cgtgctggcg ggctggggcc ttctcctttg
     1861 ggacgggcgc gcgctcgagg cgctgagcct cggtgaaacg acggcgtcga gcctcgggat
     1921 ctctctcagg aggcttcgcg cgagggtcgt gctcgggacc gcgatcgagg tcggcgcgtc
     1981 ggtcgcg
//
LOCUS       DAOOBX010000174         1985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_62856_length_1985_cov_3.885492, whole genome shotgun sequence.
ACCESSION   DAOOBX010000174 DAOOBX010000000
VERSION     DAOOBX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62856_length_1985_cov_3.885492"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PLY30_03100"
     CDS             <1..813
                     /locus_tag="PLY30_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPW65125.1"
                     /translation="AKTRDITGGLPRVAELFEARKPKNPAIISEIDGVIEFGESVKGQ
                     RKIIVKSPSGMAKEYLIPHGKHLNVYKGDKVLAGQQLVDGPVVPQDILRVSGERSLQA
                     YLVGEVQEVYRLQGVKLNDKHVEVIVRQMLRKVRIEEPGDTDFLVGSHVEKLKFAEEN
                     AKMLKKGKKPAIGTPILLGITKASLTTDSFISAASFQETTRVLTEAASAGKRDFLQGL
                     KENVIMGHLIPAGTGFKAHQNIEIEKDLTDVLPDPEALKAKAVEETASKEKE"
     gene            813..1208
                     /gene="rpsL"
                     /locus_tag="PLY30_03105"
     CDS             813..1208
                     /gene="rpsL"
                     /locus_tag="PLY30_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012620078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPW65126.1"
                     /translation="MAPTINQLIKYGRRRFQSKSKSPALHNAPFRKGVCLIVRTQTPK
                     KPNSALRKIARVRLTNGEEVSAYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGK
                     LDTSGVADRTKSRSKYGAKTPKQKDAKAA"
     gene            1205..1672
                     /gene="rpsG"
                     /locus_tag="PLY30_03110"
     CDS             1205..1672
                     /gene="rpsG"
                     /locus_tag="PLY30_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPW65127.1"
                     /translation="MRRRRAAKRRIDPDPRFNDLLVAKLINIVMECGKKSIAEKIVYQ
                     AFDIIREKTGKEPLEVFHQSMDNIRPLLETKSRRVGGATYQVPVSVPEERGNSLALRW
                     IRGYSRERKGKPMHEKLAQELIDGYNKTGASIKKKEDVHKMAESNRAFAHYRW"
     gene            1754..>1985
                     /locus_tag="PLY30_03115"
     CDS             1754..>1985
                     /locus_tag="PLY30_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPW65128.1"
                     /translation="MADEKKIHYPLEKIRNIGIAAHIDAGKTTTSERVLYYTGRTYKI
                     GEVHEGTAVMDWMEQEQERGITIASAATTCFWR"
BASE COUNT          513 a          543 c          573 g          356 t
ORIGIN      
        1 gcgaagaccc gcgacatcac cggcggtctt ccgcgcgtgg ccgaactgtt cgaggcccgc
       61 aaaccgaaga atcccgccat catttccgag atcgacggcg tgatcgaatt cggcgagagc
      121 gtgaaggggc agcgcaagat tatcgtcaag agtccatcgg gaatggcgaa ggaatatctc
      181 atcccgcacg gaaagcacct gaacgtctac aagggtgaca aggtcctcgc gggccaacag
      241 ctcgtggacg gtccggtcgt tccgcaggac attctccggg tttccggcga acgcagtctt
      301 caggcttacc tcgtcggcga agtgcaggaa gtctatcgtc tacagggtgt gaagctgaac
      361 gacaaacacg tcgaggtgat tgtccggcag atgcttcgca aggtccgcat cgaagagccg
      421 ggcgacacgg atttcctcgt cggctcccac gtcgaaaaac tcaagttcgc ggaagagaac
      481 gccaagatgc tgaagaaggg caagaagccg gccatcggca cgcccatcct tctcgggatc
      541 acgaaggcgt ccctcacgac cgacagcttc atctcggcgg cgagcttcca ggaaacgacg
      601 cgtgttctca cggaagccgc gtccgcaggg aagcgcgact tcctgcaggg actcaaggag
      661 aacgtgatca tggggcatct gatccccgcg ggcaccggtt tcaaggccca tcagaacatc
      721 gagatcgaaa aagacctgac cgatgttctt ccggatccgg aagccctgaa ggcgaaagcc
      781 gttgaagaga cagcctcaaa ggagaaggaa taatggcacc gactatcaat cagctcatca
      841 agtacggcag acgccgtttt caatcgaagt ccaaatcacc ggcgttgcac aacgctccgt
      901 ttcgcaaagg cgtctgcctg atcgtccgta cgcagacccc caagaagccg aactcggcgc
      961 ttcgcaagat tgcccgtgtc cgtctcacga acggggaaga agtctcggcc tatatcccgg
     1021 gcgtcggaca caatctgcag gaacactcaa tcgtgctcgt tcgcggcggc cgcgtcaagg
     1081 accttcccgg cgtccgctat cacatcgttc gcggtaaact cgacacctcc ggggtcgcgg
     1141 accgcactaa atcacgctcc aagtacgggg ccaaaacacc caagcagaag gatgcaaagg
     1201 cagcatgaga aggcgtcgcg cagcaaaaag aaggattgat ccggacccgc ggtttaacga
     1261 tcttctggtc gcaaagctca ttaacattgt gatggagtgc gggaagaagt cgatcgccga
     1321 aaagatcgtc tatcaggctt tcgatattat cagggaaaaa accggaaaag aaccacttga
     1381 agttttccat caatctatgg ataatatccg gccccttttg gaaacgaagt cccgccgagt
     1441 cggtggggca acctaccagg tgccggtcag tgttccggaa gagcgcggta attcactcgc
     1501 gcttcgttgg atccggggct attcccgcga gaggaagggg aaacccatgc acgagaaact
     1561 cgcgcaggaa ttgatcgacg gctacaacaa gaccggcgca tcgatcaaga agaaagaaga
     1621 cgttcataaa atggcggagt ccaacagagc gttcgctcat tatcgctggt aatcataaca
     1681 gggagtcggg agtttttgga tcggggcaag ggagtttttc atagctccgg gctcatagct
     1741 caaaattaaa tttatggcag acgaaaagaa gattcattat ccgctggaaa agatccgcaa
     1801 tatcggaatt gcggcgcata ttgatgcggg caagaccacg acctcggagc gtgtacttta
     1861 ttacaccggc cgcacgtaca agatcggcga agtgcacgag ggaaccgcgg tcatggactg
     1921 gatggaacag gaacaggagc gcggcatcac gatcgcctca gccgcgacga cgtgtttctg
     1981 gaggg
//
LOCUS       DAOOBX010000175         1978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_63177_length_1978_cov_3.319293, whole genome shotgun sequence.
ACCESSION   DAOOBX010000175 DAOOBX010000000
VERSION     DAOOBX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63177_length_1978_cov_3.319293"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..402)
                     /locus_tag="PLY30_03120"
     CDS             complement(31..402)
                     /locus_tag="PLY30_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65129.1"
                     /translation="MKLGRTIVICLLFALAIAVYLYRARIDRDILAISPDEVSRGFSM
                     EPGEQVIRIEIRNPAAGTALTLEKVRETWRIVSPVNSLAEQQAVSGLAVALRFASQQT
                     RLRAEKDWAEYGLEKPGMEVR"
     gene            complement(399..1814)
                     /locus_tag="PLY30_03125"
     CDS             complement(399..1814)
                     /locus_tag="PLY30_03125"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HPW65130.1"
                     /translation="MKHFFLKFQLVLEAVLLFATLVLVGAILDRYDYRWDLTAEKVYS
                     LPKSTASILRDLRSKPLQVSAFYLQDDPARRGLEIFLRECQRQHPDFHFDFYDPVRRP
                     TLAKKFQVTTPATIIIRSGDHEERIVTPNEEAFTNAFLRILHPRTVDVCFVKGHGEVE
                     LRGDEPVTYDMFRTTLEGFNTRVNEIVLSRDHVPDACQVVALAGPRWEPTPEEFDDLK
                     KAFRQGKGLLLLLDPMDPGTGKAFPAFAKSMGVALGDNVVVDKASRIVGGDFLMPLVS
                     QYFVRHPVSKTMKQATFFPLLRSVQPSTDTVPGFEVVPLAMTGPGSWAESNLSALEDG
                     TVTFDPKSDIAGPLPAAIAVEQITDGKQKHAAITSEEAPAADNGPETGGRMIIVGDSD
                     FLTNGYLSLSGNKEFGLRIFQWLAKDDRFIEVQKPQFKFKPLLLDIPKQSLLVFSVLA
                     VYPLLFIVIGGLYLVIRLRTS"
BASE COUNT          450 a          586 c          558 g          384 t
ORIGIN      
        1 caattaaact tcgtctcgca tgtacaagtt ttagcggact tccatgccgg gcttctcgag
       61 cccgtattcg gcccagtcct tctcggcgcg aagccgggtc tgttgcgagg cgaaacgcaa
      121 agctaccgca agtcccgata ctgcctgctg ctcggcaaga ctgttcaccg gcgaaacgat
      181 gcgccatgtc tcccggacct tttccagggt gagcgcggtt cccgccgccg ggttccggat
      241 ctcgatccgt atcacctgct cgcccggttc catcgagaac ccccggctca cctcgtcggg
      301 actgatcgca agaatgtccc tgtcaatcct tgcccggtaa aggtagaccg cgatcgcaag
      361 ggcgaacaac agacagatga cgatcgtccg gccgagtttc atgacgtgcg cagccggatc
      421 acaagataga ggccgccgat cacgataaaa agcagcggat agaccgccag gaccgagaac
      481 accagcaggc tttgtttcgg gatatcgagg agaagcggct tgaacttgaa ctgcggtttc
      541 tggacctcga taaagcggtc gtccttggcc agccactgga agattctgag accgaactcc
      601 ttgttcccgg aaagactgag ataaccgttg gtgagaaaat cgctatcacc aacgatgatc
      661 atcctgccgc cagtttccgg tccattgtct gcggccggcg cctcttccga cgtgatggcc
      721 gcatgcttct gctttccgtc cgtgatttgt tcgaccgcga tcgccgcggg cagcggcccg
      781 gcgatatccg acttcggatc aaaggtcacg gtcccgtctt caagcgcgga caggttcgat
      841 tccgcccagc tcccaggccc cgtcatggcg agcgggacca cctcaaatcc cggcaccgta
      901 tcggtcgagg gctggacgct tcgaagcaac ggaaaaaaag tcgcctgttt catggtcttc
      961 gacaccggat gacggacaaa atactggctg acgagcggca tcagaaaatc gccgccgacg
     1021 atccggctgg ccttatcgac caccacattg tcgccgagcg cgacccccat cgacttcgca
     1081 aaagccggga aggcctttcc agtccccgga tccatcggat caaggagcaa aagaagcccc
     1141 tttccttgcc ggaacgcttt tttcagatca tcaaattcct ccggggtggg ctcccatcgc
     1201 ggacccgcga gtgcaaccac ctgacaggca tcggggacat gatcgcggga aagaacgatt
     1261 tcgttcacgc gcgtattgaa accttcaagg gtcgtccgaa acatgtcgta ggtaacgggt
     1321 tcgtcgcccc gcaattcgac ctcgccatgt cccttcacaa aacagacatc caccgtccgc
     1381 gggtgcagga tccgcagaaa cgcgtttgtg aacgcctctt cgttcggcgt cacgatccgt
     1441 tcctcgtgat cccccgaacg gatgatgatt gtcgcgggtg tcgtcacctg gaatttcttg
     1501 gcgagggtgg gacggcgcac gggatcgtaa aaatcgaaat gaaaatccgg atgctggcgt
     1561 tggcattccc tcaaaaagat ctccagaccg cgccgcgcgg ggtcatcctg aaggtagaag
     1621 gccgagacct gaagcggttt ggatcggaga tcccggagga tactcgccgt gcttttcggc
     1681 aacgagtaga ccttctccgc cgtaaggtcc cagcgatagt cgtaacgatc caggatcgcg
     1741 cccaccagca cgagcgtcgc aaaaagcagc accgcctcaa gcaccagctg gaatttgagg
     1801 aagaaatgtt tcattcgaaa tgcttccagt tgcgggtttc gatcgagcgg agggaaagga
     1861 acaggaaata aaagaaaaag aaaccgaaat agatgatgtc acggaggtcc aggatcccgt
     1921 acgtgaggtt acggaagtgc tgaaggggcg acatggcgct gaggatcacg ggcacccc
//
LOCUS       DAOOBX010000176         1974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_63353_length_1974_cov_3.331944, whole genome shotgun sequence.
ACCESSION   DAOOBX010000176 DAOOBX010000000
VERSION     DAOOBX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63353_length_1974_cov_3.331944"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /gene="rplO"
                     /locus_tag="PLY30_03130"
     CDS             <1..308
                     /gene="rplO"
                     /locus_tag="PLY30_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010279632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPW65131.1"
                     /translation="PGFEGGQNPFYRLLPKRGFNNAEFKTTYAIVNLDQIAAVAEKEI
                     TPELLVGRGIVKNLRDGVKILGTGDLKGAVTVKAHSFSASAKSKIEAKGGQAILLGK"
     gene            337..1686
                     /gene="secY"
                     /locus_tag="PLY30_03135"
     CDS             337..1686
                     /gene="secY"
                     /locus_tag="PLY30_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPW65132.1"
                     /translation="MLQAFGNILKIPDLRKKIFFTFMIIAIYRVGTYVPTPGIDGVAL
                     ARFFQQNMGGGNLLGFMGMFTGGALQKATIFALGIMPYISSSIILQLLTVVIPALEKM
                     AKEGEEGRKKIIQLTRYGTIILSILQTFFIALWLENPAAFNGTVIVPNPGWGFRLMCM
                     LTMTTGTAFIMWLGEQIDAYGIGNGMSIIITAGIIEALPEALRQTWILLSPLDPSRQQ
                     LPWWKFGIMCALFVGAVLFLILIIQGQRKIPVQYAKQIRGHRVYGGQSTYLPLKVNQA
                     GVIPIIFAQSVILFPSTLAGFFPKAGMLTKVAQLLAYGTVTYSVIYALGIVFFTFFYT
                     AITFNPIEVSDNLQKYGGFIPGIRPGKTTAEYLDYLMTRIAMAGAVILALIALFPSII
                     SSQYVLNIPFLVASFFGGTGLLIIVGVMLDVMRSIESQLIMRHYDGFIKKGRLKGRR"
     gene            1689..>1974
                     /locus_tag="PLY30_03140"
     CDS             1689..>1974
                     /locus_tag="PLY30_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPW65133.1"
                     /translation="MNLIFLGPPGAGKGTHAEMLSKSMRLSHLAAGDILRRNIREGTA
                     LGMQARDIMARGELVPDTLVNNMMFAEIRELRAKGAKGFILDGYPRTIVQA"
BASE COUNT          398 a          571 c          556 g          449 t
ORIGIN      
        1 gtcccggttt tgaaggcgga cagaacccgt tttatcgcct tctgccgaag cgcggtttca
       61 ataacgctga gttcaaaacg acgtacgcca ttgtcaatct cgaccagatt gccgctgtcg
      121 ccgaaaaaga gatcacgcct gagcttctcg tcgggcgtgg gatcgtgaaa aatctccggg
      181 acggtgtcaa aatcctcggc acaggcgacc tcaaaggcgc cgtgaccgtg aaggctcaca
      241 gtttcagcgc ttccgccaag tccaagatcg aggccaaggg cggccaggcg atcctgctcg
      301 gtaaataaaa attaaataaa gaagaacgga gttctggtgc ttcaagcgtt cggtaacatc
      361 ctcaagatcc ctgatcttcg caagaagata ttcttcacct tcatgatcat cgcgatctac
      421 cgcgtcggga cgtatgttcc gaccccgggg atcgacggcg tcgcgctcgc gcgtttcttt
      481 cagcagaaca tgggcggcgg gaatctcctc ggcttcatgg ggatgttcac cggcggcgcg
      541 ctccagaagg cgactatttt cgcgctcggc attatgccgt acatctcgtc ctcgatcatt
      601 ctgcagctcc tcacggtcgt catcccggcg cttgagaaga tggcgaagga gggtgaggaa
      661 ggccgcaaaa agatcataca gttgacccgc tacggcacga tcatcctcag tatcctgcag
      721 acctttttca tcgcgttgtg gctcgagaac ccggccgcgt tcaacggcac ggtcatcgtt
      781 ccgaaccccg gttggggttt ccggctcatg tgtatgctga ccatgacgac cggcacggct
      841 ttcatcatgt ggctcggtga gcagatcgac gcctacggca tcggaaacgg tatgtcgatc
      901 attatcaccg ccggtatcat cgaagcgctt cccgaggcgc ttcgccagac ctggattctt
      961 ctttcgccgc tcgatccgtc ccgccagcag ttgccgtggt ggaagttcgg gatcatgtgt
     1021 gcgcttttcg tcggcgcggt cctctttctg attctcatca tccaggggca gcgaaagata
     1081 ccggttcagt acgcgaaaca gatccgcggc caccgcgtgt atgggggcca gagcacgtac
     1141 cttccgctca aggtaaacca ggcgggtgtc attccgatca ttttcgcgca gtccgttatc
     1201 cttttcccgt ccacgctcgc ggggttcttc ccgaaggccg gcatgctgac caaggtagcg
     1261 cagctgcttg cgtacgggac cgtcacgtat tcggtgatct acgccctcgg catcgtgttc
     1321 ttcaccttct tctacactgc gatcacgttt aacccgatcg aggtgagcga caaccttcag
     1381 aaatacggcg gtttcattcc gggaattcgt cccggcaaga cgacggccga gtatctcgat
     1441 tatctgatga cccggatcgc gatggcgggc gccgtgatcc tggccctgat cgcgcttttc
     1501 ccgagcatca tttcttcgca atacgtgttg aacatcccgt tcctcgtcgc gagctttttc
     1561 ggcggtaccg gtcttctcat tatcgtcggc gtcatgctcg atgtgatgcg ttcgatcgaa
     1621 tcgcagctta ttatgcgtca ctatgacggg tttatcaaaa agggccgcct gaaaggtcgc
     1681 agataatcat gaatctcatt ttcctgggac ctcccggagc gggaaagggc acgcacgcgg
     1741 aaatgctcag caagtcgatg cggctttcgc acctcgccgc gggagacatc cttcgacgga
     1801 acatcaggga aggcacggcg ctcgggatgc aggccaggga tatcatggcc cgcggggagc
     1861 tcgttccgga cacgctcgtc aacaatatga tgtttgccga gattcgcgag ctcagggcaa
     1921 aaggggcgaa gggtttcatc ctcgacggtt atccgaggac gatcgtgcag gcgc
//
LOCUS       DAOOBX010000177         1962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_63873_length_1962_cov_3.672784, whole genome shotgun sequence.
ACCESSION   DAOOBX010000177 DAOOBX010000000
VERSION     DAOOBX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63873_length_1962_cov_3.672784"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1962)
                     /gene="ligA"
                     /locus_tag="PLY30_03145"
     CDS             complement(<1..1962)
                     /gene="ligA"
                     /locus_tag="PLY30_03145"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPW65134.1"
                     /translation="MLGWIAERVPGLVKEIVSRGHEVASHGYNHQLNYNLDSNALRKD
                     LERHNRLYYLDARPEIPDFEYDKRMRELADLERAFPELLTPDSPSQRVGGAPLESFRS
                     VAHAVPMLSLDNTYSREELADFDDRVAKVLGAGKYAYFAEEKIDGVSITLVYEKGFLK
                     LGATRGDGKRGDDITENVKTIGSVPLRIPVEGSSFRGKVPDLLEVRGEAYIPKGQFEK
                     INAGKEKNGEELFANPRNACAGSLKLLDPKLVRARKLDAFIHGMARCEGEGDAPASQS
                     EAMAFLKEMGFKTIPDARKCRDIAGVFGVIDAIETKRVALPYEIDGVVVKVDRVDDQK
                     TLGFTSRSPRWMIAYKYAAERARTKLLDIRVQVGRTGVLTPVAVLEPVQLAGTTVSRA
                     SLHNRDEIKRLDARIGDTVFIEKSGEIIPQVISVDVSMRPAGAKAFDFPKTCPVCGED
                     AGKTAGEVAVRCRNPLCPAKLKAALKHYAGREAMDIENLGVSIIDQLVEKGFVKDLAD
                     LYTLKTTDLEALERMGEKSANNLTEGIEQSRTRPLANLIFGLGIPDVGVHTAFILADR
                     FGTLDALAAATAGDLEGIREVGPVTAASIVRFFRQASTAKLIKKMKAAGVRTDIVEKA
                     AGDNSFKGKTVVLTGTLEKMERSGAEALLR"
BASE COUNT          345 a          636 c          582 g          399 t
ORIGIN      
        1 gcgaagcagc gcctcggccc cggagcgttc catcttctcg agcgtgccgg tcagcacgac
       61 ggtctttccc ttgaaggaat tgtcccctgc cgccttctcg acaatgtccg tccggacgcc
      121 cgcagccttc atctttttga tcagtttcgc ggtcgacgcc tggcggaaaa agcggacaat
      181 cgaggccgcg gtgacgggcc cgacctcgcg gatgccttcc aggtctccgg cggtcgcggc
      241 cgcgagcgcg tcaagagtac cgaaccggtc cgcgaggatg aaggccgtgt gcacgccgac
      301 atcggggatg ccgagaccga agatcaggtt cgcgagcggc cgcgtccggc tctgttcgat
      361 cccttccgtg agattgttgg cggacttctc ccccatacgc tcgagcgctt caaggtcagt
      421 cgttttcagc gtgtagaggt ccgcgaggtc cttcacgaac cctttctcga cgagctggtc
      481 gatgatcgag acgccgaggt tttcgatatc catggcctcg cgccccgcgt agtgtttgag
      541 cgcggccttg agcttcgcgg gacagagcgg gttgcgacag cggacagcga cctcgccggc
      601 cgtctttccc gcgtcctcgc cgcagacggg acaggtcttc gggaaatcga acgccttcgc
      661 gcccgccggc cgcatcgaaa cgtccaccga aatgacctgg gggatgatct cgccgctttt
      721 ttcgatgaaa accgtgtcgc cgatccgcgc gtcaagacgt ttgatctcat cccggttgtg
      781 cagactcgcg cgtgacaccg tggtgccggc gagctggacg ggctcgagaa ccgcgaccgg
      841 agtaagcaca cccgtgcgtc cgacctggac cctgatatca agaagcttcg tccgggcccg
      901 ctccgcggcg tacttgtaag cgatcatcca ccgcggcgac ctggatgtga atccgagcgt
      961 cttctggtca tcgacacggt cgaccttcac aacaacgccg tcgatctcgt acggaagcgc
     1021 gacgcgcttg gtttcgatcg cgtcaatcac gccaaagaca cccgcgatgt cccggcattt
     1081 ccgcgcgtcc gggatggtct tgaaccccat ctccttcaaa aacgccatag cctcgctctg
     1141 gctcgcgggc gcgtccccct ccccctcaca gcgggccatg ccgtggataa aggcgtcgag
     1201 tttgcgcgcg cggacgagct tcgggtcgag cagtttgagg cttcccgcgc aggcattccg
     1261 gggattggcg aaaagctcct cgccgttttt ctccttcccg gcattgatct tctcgaactg
     1321 tcccttcggg atataggcct caccgcgtac ctcgaggagg tccggcacct tgccccggaa
     1381 ggaacttccc tcgacgggaa tcctgagcgg aacggagccg atcgtcttca cgttctccgt
     1441 gatatcgtcg ccgcgtttgc cgtcaccgcg cgtcgcgccg agcttgagaa accccttctc
     1501 gtacacaagc gtgatcgaga caccgtcgat tttctcctcc gcaaaatagg cgtacttccc
     1561 ggcgccgagc accttcgcga cgcggtcgtc gaaatcggcg agctcctccc tggaataagt
     1621 gttgtcgagc gagagcatcg gcaccgcgtg ggccacggac cggaaggact caagcggcgc
     1681 gccgccgaca cgctgcgagg gggaatcggg agtgaggagt tccgggaacg cccgctcgag
     1741 atccgcgagc tcccgcatcc ttttgtcgta ttcgaaatcc gggatctcgg gacgcgcgtc
     1801 gaggtagtag agccggttgt ggcgctcgag gtcttttctc aatgcattag aatcaagatt
     1861 ataattgagt tggtgattgt atccgtgaga cgcaacttcg tgcccccttg aaactatctc
     1921 tttaactaac cccggaactc tttccgctat ccagccgagc ac
//
LOCUS       DAOOBX010000178         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_64724_length_1944_cov_2.404976, whole genome shotgun sequence.
ACCESSION   DAOOBX010000178 DAOOBX010000000
VERSION     DAOOBX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64724_length_1944_cov_2.404976"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1944
                     /locus_tag="PLY30_03150"
     CDS             <1..>1944
                     /locus_tag="PLY30_03150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW65135.1"
                     /translation="WARTFESFHLSEKRFRIVSSGSLHRIDNPPSYALIIVDEAHNFR
                     NSNTEAYTQLNELCKMPGDLGRKKVILVSATPLNNHPRELLNLIKLFQDERNSTLSVT
                     DLQAFFSERQKAFDEAKKAGVDVKTANEKVKTIYREIRELVLADITIRRTRNDLKDNK
                     LYAKDLADQGIVFPETQSPRTVPYYLDSDVDALFTETLEILAGENGLHYAYHKMIDYV
                     RGSAREKFNLPENAFVQLKRLIVQFFLKRLDSSFHAFRSSLTRFRDDTAALMTMLDAG
                     TVYYSNSIDVRQYIANEDIAGLEAEIELKRASDPSITKLTPEDFSQEFHTHLHEDLEK
                     LFVLVERWEAVTEDPKLEMFASQIDGWLKKDKNPGERLVVFSESEDTVIHIRDHLEKT
                     GRKDVLAVSAANRNETISVTTGSDDEEMEVEELIRRNFDANYPHSLKESRYRVIVTTD
                     VLSEGVNLHRANTIVNYDTPWNSVRLFQRIGRVNRVGSVADKIFIYNFFPMSRVDDVI
                     ELKKKAQMKLQAFHSAFGEDAPIYSSDEEVEHFGLYGSGSAEDSEAANPKLKLMMEIR
                     ELAQENPAEFERIKGLPAHMRCVRASAAPMEPMLYLSTSSKDLFYRVGETEARQITLM
                     EAAGILRADSGEMPSQGEFDWDAL"
BASE COUNT          475 a          593 c          526 g          350 t
ORIGIN      
        1 ctgggcgaga acctttgagt ctttccatct ttcggagaag cggttcagaa tcgtaagctc
       61 gggcagcctt cacagaatcg acaacccacc gtcgtatgcg ctcatcatcg tcgacgaagc
      121 gcataatttc cggaacagca acacggaagc gtatacccag ctcaacgagc tctgtaaaat
      181 gccgggagac ctcggccgga agaaggtgat actcgtgtcg gcgacgccgc tcaacaacca
      241 cccccgggag cttctgaacc tcatcaagct cttccaggac gagcgcaact cgacgctttc
      301 cgtcactgat cttcaggcgt ttttctccga acggcaaaag gcgttcgacg aggcgaagaa
      361 ggctggcgtc gacgtcaaga ccgcgaacga gaaggttaag acgatctacc gcgaaatccg
      421 cgaactcgtg cttgccgaca taacgattcg ccgcacgcga aacgacctga aggataacaa
      481 gctctacgcg aaggacctcg ccgatcaggg catcgtcttc cccgaaacgc aaagcccgcg
      541 cacggtgccc tactatctag acagcgacgt ggacgcgctc tttaccgaaa ccctcgagat
      601 cctcgcgggc gagaatggcc tccattacgc ctatcacaag atgatcgact acgttcgggg
      661 atcagcgcga gagaaattca acctgcccga aaacgcgttc gtgcagctca agcggctcat
      721 cgtgcagttc ttcctcaagc ggctcgactc gagctttcac gcattccgct cctcgctcac
      781 ccggttcagg gatgacacgg ccgcgctcat gaccatgctc gacgcgggca cggtatatta
      841 ttcgaacagc atcgacgttc gccagtacat cgcaaacgag gacatcgccg gacttgaggc
      901 ggaaatagag cttaagcgcg cgagcgatcc ctcgatcacg aagctcaccc ctgaagactt
      961 ttctcaggaa tttcatacgc acctccacga agacctcgaa aagctcttcg tgctcgtcga
     1021 gcgatgggag gccgtaacgg aagacccgaa gctcgagatg ttcgcgagcc agatcgacgg
     1081 atggctcaag aaggataaga accccggcga acgcctcgtc gtgttctcgg agtccgagga
     1141 cacggtcatc cacatccgcg accacctcga gaaaaccggc cgaaaggacg tgctcgccgt
     1201 ttccgccgcg aaccgcaacg aaacgatttc cgtgacgacg ggatcggacg acgaggagat
     1261 ggaggtcgag gaacttatca ggcggaactt cgacgcaaat tatccgcaca gcctcaaaga
     1321 gagccgttat cgcgtcatcg tgacgaccga cgtcctttcg gaaggcgtca acctccacag
     1381 ggcgaacacg attgtcaatt acgacactcc ctggaactcg gtcagactct tccagcgcat
     1441 cgggcgcgtc aaccgcgtcg gaagcgtcgc ggacaagatc tttatctaca acttcttccc
     1501 catgagccgc gtggatgacg taatcgagct caagaaaaag gcacagatga aactacaggc
     1561 ctttcactcg gcgttcggcg aagacgcgcc catctactca agcgatgagg aggtcgaaca
     1621 tttcggtctc tatggctctg gaagcgccga ggattccgag gcggcgaacc ccaagctcaa
     1681 gctgatgatg gaaattcgag agcttgcgca ggaaaatccc gccgagttcg aacgcatcaa
     1741 aggccttccc gcgcacatgc ggtgcgttcg cgcctcggcc gcgccgatgg aaccgatgct
     1801 ctatctttcg acatcgtcca aagacctctt ctatcgcgtg ggcgagacag aagcgcgaca
     1861 gattaccctc atggaagcgg ccggcatcct ccgcgccgac tcgggcgaaa tgcccagtca
     1921 aggagagttt gactgggacg cgct
//
LOCUS       DAOOBX010000179         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_64857_length_1941_cov_3.268293, whole genome shotgun sequence.
ACCESSION   DAOOBX010000179 DAOOBX010000000
VERSION     DAOOBX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64857_length_1941_cov_3.268293"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="PLY30_03155"
     CDS             complement(<1..1384)
                     /locus_tag="PLY30_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW65136.1"
                     /translation="MRLKKLQLLGFKSFADKTDIFFDSGITCIVGPNGCGKSNVSDAI
                     RWVLGERSAKILRGSAMEDVIFNGTEVRKPLAFAEVSLTIDNADRGIPIEYNEVTIAR
                     RLYRTGESEYLINKTVCRLKDIHDLILDAGIGSSTYSMIEQGRIDYILKADPEERRFL
                     IEEAAGIAKYKAKKDESIRKLERTEENLKRLNDIVYEVQKNIQYAERQAKRAEKYKVE
                     LERLKGLEIRRAFHMNDHIGSQKAALDTKARTQKDELARLETILVESRALLERQEGEL
                     KGILDRFQQKESERYGILSRIDTNDQKTQFLRDKKLEIVQQRGQVLQEQDQLRQSVES
                     GHQKKSELSNLLIAQESELTQCQAQLVQAQENLRHHDALLASVKGEMEQLRQGFFEAA
                     QATTRVRNEYQHYKSLRENLDQQRKREESETSRVAAEIASWDQRLKDSVRAIEEKASG
                     FQTLEQRLATA"
     gene            complement(1489..>1941)
                     /locus_tag="PLY30_03160"
     CDS             complement(1489..>1941)
                     /locus_tag="PLY30_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HPW65137.1"
                     /translation="SDASHITAPDPSGDGAARCIINAMKDADIRPEQVDYINAHGTST
                     SLNDKCETIAIKKALGDHAYKTRISSTKSMIGHLLGAAGAVEAVACVLAIRDQIVPPT
                     INYKNPDPDCDLNYVPNQAEKTPVRVALSNSLGFGGHNACIAFKAYEE"
BASE COUNT          336 a          511 c          599 g          495 t
ORIGIN      
        1 ccgcggtcgc gagacgttgc tcgagggtct ggaatccgga ggctttttcc tcgatcgccc
       61 ggaccgagtc cttgagacgc tgatcccatg acgcgatctc ggcggcgacg cgtgacgttt
      121 cggactcctc gcgtttgcgc tgctggtcga ggttctcccg aagggatttg taatgctggt
      181 attcgttgcg gacccgggtc gtcgcctggg ccgcttcaaa gaaaccctga cgaagctgtt
      241 ccatttcacc tttgaccgag gcgagcaacg cgtcgtgatg ccggagattc tcctgggcct
      301 ggacaagttg cgcctggcat tgggtcagtt cggattcctg cgcgatcaaa aggttcgaaa
      361 gctccgattt tttctggtga ccggactcga ccgactgccg gagctggtct tgttcctgga
      421 ggacctgtcc ccgctgctgg acgatctcga gtttcttgtc gcggaggaac tgggttttct
      481 gatcgttcgt gtcgatccgc gagaggatgc cgtagcgttc ggattccttc tgctggaagc
      541 ggtcgaggat tcccttgagc tcgccttcct gccgctcaag caacgccctc gattcgacga
      601 ggatcgtttc aagacgcgcc agttcgtcct tttgggtccg ggccttcgta tcaagggccg
      661 ccttttggga accgatatga tcgttcatgt gaaaagcccg gcgaatctca agtcctttca
      721 gacgttcgag ctcaaccttg tatttttcgg cgcgtttggc ctggcgttcg gcatactgga
      781 tgtttttctg gacttcgtag acgatgtcgt tcagacgctt caggttctcc tccgtgcgtt
      841 cgagcttgcg gatggattcg tcctttttcg ccttgtattt cgcgatgccg gcggcctcct
      901 caatgaggaa acggcgctct tcgggatccg ccttgaggat gtaatcgatt cggccctgtt
      961 cgatcatgga gtaagtcgag gagccgatgc cggcgtcgag gatcaggtca tgaatatcct
     1021 tcagacggca gacggtcttg ttgatgaggt attcgctttc gccggtccgg taaagacggc
     1081 gggcgatcgt gacttcgtta tattcgatcg ggatgccgcg gtcagcattg tcgatcgtca
     1141 gcgagacttc ggcgaacgcg aggggtttgc ggacctcggt gccattgaaa atgacgtcct
     1201 ccatcgcgga gccgcgaaga atcttggcgc tgcgttcgcc gaggacccag cgaatggcat
     1261 cggagacgtt gctcttgccg caaccgttgg gaccgacaat acaggtgatg ccgctatcga
     1321 agaagatatc ggtcttgtcg gcgaatgatt tgaaacccag tagttgaagc tttttaagtc
     1381 gcatgctgcg gctttctccg gatagtcccc cgcgcgcccg gcacgaagtt cacttgccta
     1441 gatattcggc agataagggt ggtgtctgaa ggggaaaaag cttcgggatt actcttcgta
     1501 ggccttgaat gcgatgcagg cattatggcc tccgaatccc aacgagttgg ataatgcgac
     1561 ccggaccggg gttttttcgg cctggttcgg gacgtaattc agatcgcaat cggggtccgg
     1621 attcttgtaa ttaatcgttg ggggaacgat ctggtcccgg atcgcaagaa cgcacgcgac
     1681 ggcttcgacg gcgccggccg cgccgagcag atgtccgatc atggatttcg tggaactgat
     1741 gcgtgttttg taagcgtgat cgccgagcgc tttcttgatg gcgattgtct cgcacttgtc
     1801 gttcagactg gtcgatgtgc cgtgggcgtt gatgtaatcg acctgttcgg gacggatgtc
     1861 cgcgtctttc atggcgttga tgatacagcg cgctgcccca tcccccgaag ggtcgggtgc
     1921 cgtgatgtgg ctggcatccg a
//
LOCUS       DAOOBX010000180         1940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_64923_length_1940_cov_2.337401, whole genome shotgun sequence.
ACCESSION   DAOOBX010000180 DAOOBX010000000
VERSION     DAOOBX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64923_length_1940_cov_2.337401"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLY30_03165"
     CDS             <1..348
                     /locus_tag="PLY30_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPW65138.1"
                     /translation="YTYRAWIVRVLDGDTVKAVIDLGFGVRSTQVLRLRGIDAPELVS
                     ADGKKAKEFLEKKLGLAEGEGLSDRMVLVRTEKSDKYDRYLADLFYTDKNGREIYLNQ
                     EILAAGLAIRMGE"
     gene            534..1334
                     /locus_tag="PLY30_03170"
     CDS             534..1334
                     /locus_tag="PLY30_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65139.1"
                     /translation="MKFPRIVAISTVLIFAAIAAVSFTPTGFADCGDCREQGSPLYIE
                     KNMPPQIVIGKPYEYIIRVTNRTDRLVNDVSVIETLAETYVMSAALPEPSKVAGQTIQ
                     WDFGTLKPKESRTLTIQGSAKSTGSFKSCTKVIHSQSLCQGPELGMPAILLEVIDTED
                     PVQVGGTEKFYVTVTNQGNAPDENILVKMSFEENFDYVSSGGPTQAKVESAKSVEFAP
                     LASLAPGHKATWEVTAKALSEGDHRTSVKLTSDAIRRPVDETESTHVY"
     gene            1574..>1940
                     /locus_tag="PLY30_03175"
     CDS             1574..>1940
                     /locus_tag="PLY30_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPW65140.1"
                     /translation="MVSFLMVLAPLPARAAERPALAGDYATVKRVVDGDTVILTDGQK
                     VRLIGIDTPESRRNGKLARDMEKTRQDADTIIALGKRSAQFTRSLTEGKRVQLEYDVE
                     KRDRYGRTLAYLYTEDGMFV"
BASE COUNT          420 a          544 c          589 g          387 t
ORIGIN      
        1 tatacctacc gcgcctggat cgtgagggtg ctggacggcg acaccgtgaa ggccgtgatc
       61 gatcttggtt ttggcgttcg cagcacgcaa gtcctgcggc tccgggggat cgacgctcca
      121 gagctcgtaa gcgcggacgg gaagaaagcg aaagaatttt tagaaaagaa gctgggtctg
      181 gcggagggtg aagggctaag cgacaggatg gttctggttc gtaccgaaaa atccgacaaa
      241 tacgatcgct accttgccga ccttttttat acggacaaga acggccggga gatctatctc
      301 aatcaggaga ttctggccgc gggcctggcc atccggatgg gggagtgagc gtcacttccg
      361 caacctatcc aacctcgtag gttggaaccg gtcgggaatc agatgttgac cgggtttgac
      421 cgcggggata gactaaaaac ggtttcgcgg caaggttccc cgagccgcgg atcccctcaa
      481 caaccagagc cgggaagccg ttttccctgc caagaccttc aaggaggcac gtcatgaaat
      541 ttccaaggat cgtcgcaatc tccaccgttc ttattttcgc cgcgattgcc gccgtttctt
      601 tcaccccgac gggatttgcg gactgcggtg attgcaggga gcagggctcc ccgctttaca
      661 tcgaaaaaaa catgccgccg cagatcgtga tcggaaagcc ctacgaatac ataatccgcg
      721 tcacgaatcg cactgaccgg ctggtcaacg atgtttcggt gatcgagacc ctggccgaga
      781 cgtatgtgat gagcgccgcg ttgccggagc cttccaaagt ggcggggcag acgatccagt
      841 gggatttcgg gaccctgaag ccgaaggaat cccggacgct cacgatccag gggagcgcga
      901 aatcgaccgg ttccttcaaa tcatgcacaa aggtgatcca cagccagagc ctttgccagg
      961 gccctgagct gggcatgccg gcgattcttc ttgaagtgat cgacacggag gaccccgtcc
     1021 aggtcggcgg gacggagaag ttctacgtta ccgtgaccaa ccaggggaat gcccccgacg
     1081 agaacatcct cgtgaagatg agtttcgagg agaacttcga ttatgtttct tcgggcggcc
     1141 ccacgcaggc aaaggtggaa agtgccaaat ccgttgagtt tgctccgctc gcgtctcttg
     1201 ccccgggcca caaggcgacc tgggaagtga cggcgaaggc cctctcggaa ggcgatcatc
     1261 gcacctcggt caagctgacg agtgacgcga tccggaggcc ggtggacgag accgaatcca
     1321 cgcacgttta ttgatccgga atccaccgtt tccagcctcg caggttgacg gtggttaaac
     1381 gggttcctcg gcccctgttc cgctcccgcg gagcaggggc ttttttatat gcatgggata
     1441 ccgttccctc aaggcgattg tccgggtgat tttgagaaac ttgtagcaag tcgacgataa
     1501 aagattggct gtccgctttg tgcgctccgt tttataatca ttccttatga gaatacggtt
     1561 cccgtttttt ttgatggttt ccttcctcat ggtcctcgcc ccgctgcccg cgcgcgcggc
     1621 ggagagaccc gccttggccg gggactacgc tacggtcaag cgggtcgtgg acggggacac
     1681 ggtcatcctc acggacggac aaaaggtccg gctgatcgga atcgacacgc cggaatcccg
     1741 ccggaacggg aaacttgccc gcgacatgga aaagacccgt caggacgcgg acacgatcat
     1801 cgcgctcggg aaacgctcgg cgcagttcac gcggtcgctg acggaaggga agcgcgtcca
     1861 gcttgagtac gacgtcgaaa aacgcgaccg ctacggccgc acgctcgcct atctttacac
     1921 ggaagacggg atgttcgtga
//
LOCUS       DAOOBX010000181         1937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_65049_length_1937_cov_2.366631, whole genome shotgun sequence.
ACCESSION   DAOOBX010000181 DAOOBX010000000
VERSION     DAOOBX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65049_length_1937_cov_2.366631"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /gene="argS"
                     /locus_tag="PLY30_03180"
     CDS             complement(<1..1165)
                     /gene="argS"
                     /locus_tag="PLY30_03180"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPW65141.1"
                     /translation="MKSKIDSVLNEAIESLFKDQKMDLPAGLSCDLQPPRESSHGDLS
                     TNVAFKLSKLLRKKPADIAEAIVAFIEELGENSPVEHAKIAGGGFINFSLRKADLGAV
                     LVAIYHADTRYGASEYGKGKKVIVEFVSANPTGPLTIAHGRQAAVGDSLCRILKATGH
                     EVHPEYYLNDAGRQMGLLAKSVWTRYNELLGLAMPLPEDGYKGDYIREIARDIIEKEK
                     KDLLLKEPEDKAVEFCGHYAVKAIMGRIEEDLAKMGVHFDNYFSEKSLYARKSVERAL
                     EVLKQKGYLFENEGALWFRSTDFGDDKDRVVKKSTGEYTYLAPDIAYHLSKYERGYNM
                     MVNFWGPDHHGYIPRLKAACQALGYSPEAIHIGIVQLTTLYREGQPVRMSTRAG"
     gene            complement(1241..1573)
                     /gene="rsfS"
                     /locus_tag="PLY30_03185"
     CDS             complement(1241..1573)
                     /gene="rsfS"
                     /locus_tag="PLY30_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPW65142.1"
                     /translation="MQPKRIAALIRALAEDKKAEDLVVLDISKLSSLTHYFIIAHGNS
                     DRHVRALAEHIMDEMKKKKIPALHAEGLREGKWILLDFGSVIAHIFYKDTREFYGLER
                     LWGEAKKI"
     gene            complement(1610..>1937)
                     /locus_tag="PLY30_03190"
     CDS             complement(1610..>1937)
                     /locus_tag="PLY30_03190"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPW65143.1"
                     /translation="MTGGRPSIAPLLCRSRRAKLDLSSCTPFQKKIYRTLRNVPAGRK
                     ITYGDLARRAGHPGAARAVGSAMNRNPLPVVIPCHRVVPATGGIGHYSVGVAWKRFLL
                     SCEGVL"
BASE COUNT          387 a          558 c          545 g          447 t
ORIGIN      
        1 cgcccgcgcg ggtcgacatg cggaccggct gtccttcgcg gtaaagcgtc gtcagctgga
       61 cgataccgat gtggatcgcc tccggtgaat agccgagcgc ctgacaggcg gccttcaggc
      121 gcgggatgta accatgatgg tccgggcccc agaaattcac catcatgttg tagccgcgtt
      181 cgtacttcga aagatgatag gcgatgtcgg gcgcgaggta ggtatactcc cccgtcgatt
      241 tcttcacgac gcggtccttg tcgtccccga aatcggtcga gcggaaccag agcgcgccct
      301 cattctcaaa aaggtaccct ttttgcttga ggacttcgag tgcgcgctcg acggatttcc
      361 tggcgtacag cgacttctcg ctgaaataat tgtcaaaatg gacgcccatc ttcgcaaggt
      421 cctcttcgat ccggcccatg atcgccttca ccgcgtaatg gccgcagaac tcgaccgcct
      481 tgtcctcggg ttctttcagg agaaggtcct tcttttcctt ttcgataatg tcccgggcga
      541 tctcgcggat gtaatctcct ttgtagccgt cttccggcaa aggcatcgcg aggccgagca
      601 gttcgttata acgggtccag acgctttttg cgagcaggcc catctgacgg ccggcatcgt
      661 tcaggtaata ttcgggatgg acctcgtggc cggtggcctt gaggatgcgg cagagcgaat
      721 cgccgaccgc agcctggcgt ccgtgcgcga tcgtgagcgg tccggtcgga ttcgcgctga
      781 cgaattcgac gatgaccttt ttgcctttcc cgtattccga agcaccgtag cgcgtgtccg
      841 catgataaat ggccacgagc acggcgccga gatcggcctt gcgaagggag aaattaatga
      901 atccgccgcc cgcgatcttc gcatgttcca cgggggaatt ttccccaagc tcctcgataa
      961 aggcgacgat cgcctccgcg atgtccgcgg gtttcttcct caggagcttc gacagtttga
     1021 acgcgacgtt tgtcgacaga tcgccgtggc tggattccct cggcggctga agatcgcacg
     1081 acagacctgc gggcaaatcc atcttctgat ctttaaaaag actttcgatc gcttcgttga
     1141 ggactgaatc tatctttgat ttcatatttg ggcacgggat gcggtgtacg aagcacgggg
     1201 aaaaaacgtc tgtttcatat tcgggactcc atacgccaat ctaaatcttt tttgcttctc
     1261 cccaaagccg ttcaaggccg tagaattcgc gggtgtcctt ataaaagata tgcgcgatga
     1321 ccgacccgaa gtcgaggagg atccattttc cctcccggag cccttccgca tggagagccg
     1381 gtattttctt ttttttcatc tcgtccatga tgtgctcggc aagcgcccgc acgtggcgat
     1441 cgctgttccc gtgggcgata atgaaataat gcgtgaggct ggaaagcttg gaaatatcga
     1501 gaacgacgag atcttcggcc tttttatcct ccgcaagggc gcggatcaaa gcggctattc
     1561 gtttcggctg caatgtcttg actcaccttt ccggttaaaa tttcggggat tatagcacac
     1621 cttcgcagct cagcaaaaac cgtttccatg ccaccccgac cgagtaatgt ccaatcccgc
     1681 cggtagccgg cacgacgcgg tgacacggaa tcacgaccgg caaaggattc cggttcatgg
     1741 cactcccgac cgcccgcgcc gcgccgggat gacccgcccg ccgggccaga tctccgtatg
     1801 tgattttccg gcccgccggg acattacgca gcgtccggta aatcttcttc tggaatggtg
     1861 tgcaggaaga gagatcgagt ttcgcgcggc gggaccggca aagaagaggg gcgatcgaag
     1921 gccgtcctcc cgtcatg
//
LOCUS       DAOOBX010000182         1933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_65226_length_1933_cov_2.384452, whole genome shotgun sequence.
ACCESSION   DAOOBX010000182 DAOOBX010000000
VERSION     DAOOBX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65226_length_1933_cov_2.384452"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="PLY30_03195"
     CDS             <1..1487
                     /locus_tag="PLY30_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HPW65144.1"
                     /translation="DAGHSRIAVLRDISLGLREPKFNDPVTIPKFHFLDPTLNETQKK
                     AVCLAAETKDILLIHGPPGTGKTRVLVEIIRQAVAKGEGVLVSAPSNAACDNLVEWLA
                     FHKTPVTRLGQPARVSERIREYTLSYKLAAHPYAKMIEENEARLDQLSKRKERRLVRR
                     TYQWEERKEMRTEIEQLRKDIRDLKAQIFQQVWNASDVVVATHVGAADPLIKAKAFDW
                     VVLDEATQGIEPATWIPLLHAGRVIMAGDHCQLPPAVHSSNEGKDSLGFTLFERLHEK
                     LGEASKVRLERQYRMHERIMDFPSREFYDKKLVADDSVKRHALTDLPFIKGKDLDLTP
                     AVFLDTAGLGHEEETEEGTGSRSNLEEARLVVRELGRLLDAGVRPIDIAVISPYSAQV
                     KLLTGMIIGDNQDPGEIADLEIDSVDAFQGREKEAVIVSLVRSNTEGEIGFLADTRRM
                     NVAMTRAKRKLILVGDSATLCRLPFYDNLLKYMESINGYRSAWE"
     gene            1630..1839
                     /locus_tag="PLY30_03200"
     CDS             1630..1839
                     /locus_tag="PLY30_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65145.1"
                     /translation="MIALPGFVLGAVSAFRPERSIAFYQRIMKHFNWRAEPIDAAKEL
                     RNTRVLGLWLAALNLALAVFAGMKF"
BASE COUNT          421 a          516 c          592 g          404 t
ORIGIN      
        1 aggacgccgg tcattcccgg atcgcggtac tccgtgatat cagccttggg cttcgggagc
       61 caaaattcaa cgatccggtg acgatcccga aattccattt cctggatccg accctgaacg
      121 agacccagaa gaaggcggtc tgtctggcgg ccgagaccaa agacatcctt ttgatccacg
      181 gtcctcccgg aaccggcaag acgcgcgtgc tggtcgagat catccgacag gccgtcgcga
      241 aaggggaggg cgtcctcgtg tcggccccgt cgaatgcggc gtgtgacaat ctcgtggagt
      301 ggttggcttt tcacaaaacc cccgtgacgc gtctcggaca gccggcgcgc gtgagtgagc
      361 ggatccggga gtatacgctt agttacaagc tggccgcgca tccttacgcg aagatgatcg
      421 aggagaacga agcccgcctt gaccagttat cgaagcggaa ggagcgcagg cttgtccgcc
      481 gcacctatca atgggaggag cggaaggaga tgcgcaccga gatcgaacag ctccgcaagg
      541 atatccgtga cctcaaggcc cagatctttc agcaggtctg gaacgcttcc gatgtcgtgg
      601 tcgcgacgca tgtcggcgcg gcggatccgc tgattaaggc gaaggccttc gattgggtgg
      661 tccttgacga agcgacacag ggaatcgagc ccgcgacctg gattccgctc cttcatgcgg
      721 gccgggtgat catggcgggc gatcattgtc agttgcctcc ggcggtgcat tcctcaaatg
      781 aaggaaaaga ttcgctcgga ttcacgctct ttgagcgcct tcatgaaaaa ttgggcgaag
      841 caagcaaggt gcggctcgaa cggcaatacc ggatgcacga aaggatcatg gatttcccgt
      901 cacgggaatt ctatgacaag aaactcgtcg cggatgattc ggtcaagcgg catgccctga
      961 ccgacctgcc ttttatcaaa gggaaggacc tggacctcac gcccgcggtg ttcctggata
     1021 cggccgggct cggccatgaa gaggagaccg aagaaggtac cgggagtcgt tcgaacctgg
     1081 aagaagcgag gctggtcgta agggaactcg gtcggctcct cgatgcgggc gtacgaccga
     1141 tcgatattgc ggtgatcagc ccttacagcg ctcaggtgaa actgttgacg gggatgatta
     1201 tcggggacaa ccaggatccg ggcgagatcg cggacctcga gatcgattcg gtggatgcgt
     1261 ttcagggtcg cgaaaaggag gccgtgatcg tgtcgctcgt gcggtccaac acggaagggg
     1321 agatcggatt cctcgcggac acgcggcgca tgaatgtcgc gatgacacgc gcgaaacgga
     1381 agttgatcct tgtcggagac agcgcgacgc tttgccgttt gcctttttat gacaacctgc
     1441 ttaaatatat ggagtctatc aacggttatc gaagcgcgtg ggaatgaaac attgggactc
     1501 ataccccgaa cctcgagact tgcgcgattc aaggccggtt ccgggttccc gggaacgagc
     1561 cccgggaggt aaattatgca atgtccgttg ccaggttgtt ttgttttttc gcccaaggtg
     1621 atttttcttg tgatcgcgct gcccggtttt gtgctgggag cggtctccgc tttccgtccg
     1681 gagcgatcga tcgcctttta ccagcggatc atgaaacatt tcaactggag agccgaaccg
     1741 attgacgcgg cgaaggaatt gcgcaatacc cgggttctcg gcctgtggct cgcggctctc
     1801 aatcttgccc ttgccgtgtt tgccggtatg aaattttgag tcgtcccgtc aaataacgag
     1861 cccggaaaac aagccttgac aagagggaac tcccgcctat aatcccttcc ttattaagac
     1921 tcagtctgaa taa
//
LOCUS       DAOOBX010000183         1932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_65264_length_1932_cov_2.488546, whole genome shotgun sequence.
ACCESSION   DAOOBX010000183 DAOOBX010000000
VERSION     DAOOBX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65264_length_1932_cov_2.488546"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /gene="recA"
                     /locus_tag="PLY30_03205"
     CDS             <1..724
                     /gene="recA"
                     /locus_tag="PLY30_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPW65146.1"
                     /translation="DPVYAQKIGVKVDDLLISQPDSGEQALQIAEMLVRSNAVDLIVV
                     DSVAALVPKAEIEGEMGQSQMGLQARLMSQAMRKLTAIINKSKTCIIFINQLREKIGV
                     MFGNPETTPGGKALKFYASMRLDIRRIASLKKGDEIIGNRVRVKIVKNKVAAPFRQAE
                     FDILFSEGISRSSNLLELGVMYQVIEKSGTWLAYNGEKLGQSKDAARMFLKENPKLLE
                     EIESRIIEKVAAGETATVGVAD"
     gene            743..1243
                     /locus_tag="PLY30_03210"
     CDS             743..1243
                     /locus_tag="PLY30_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPW65147.1"
                     /translation="MAEPPDEKSKQALITALRLLAASPKSQRELRTKLEAKGYPVMAV
                     QFAMNELSGQGVLDDKAYAKNLTTRLVQGSAAGRRRVAFELKHHGVPEKVQREILGSL
                     TESNERARALELARSKWPSLSKLEPAKRKKKLFDHLIRKGYDYHVVRDVLETVARDTP
                     DDDPTL"
     gene            1240..>1932
                     /locus_tag="PLY30_03215"
     CDS             1240..>1932
                     /locus_tag="PLY30_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HPW65148.1"
                     /translation="MKDQYTADAIRKAYLDFFASRGHTVTPSDSLVPQNDPSLLFTGA
                     GMNQFKEYFLGVKKDLKRAVSSQKCLRTGDLDEVGRTPYHHSFFEMLGNFSFGDYFKK
                     EAIIWAWEFLTAILSIPKERLRITVHENDQEACDLWRKEIGLPEEWIYKCGDKSNFWP
                     ANAPKDGPNGPCGPCSEIYYDLNPSITGTPVDDSGKYAEIWNLVFTQYDRQEGGKLVP
                     LKARNIDTGMGLE"
BASE COUNT          474 a          577 c          534 g          347 t
ORIGIN      
        1 tgatcccgtc tacgcccaga agatcggcgt caaggtcgac gaccttctga tctcgcagcc
       61 cgactcgggt gagcaggctc tccagatcgc agagatgctg gttcgttcca atgccgtgga
      121 cctgatcgtg gtcgactccg tcgccgcgct cgtcccgaag gccgagatcg agggggaaat
      181 gggccagtcc cagatgggcc tccaggcccg tctgatgtct caggcgatgc gcaagctgac
      241 agctatcatc aacaagtcga aaacctgtat catcttcatc aaccagctcc gcgagaagat
      301 cggcgtcatg ttcggaaatc ccgagaccac accgggcggc aaggccctga agttctacgc
      361 ctccatgcgt ctggatatcc gccggatcgc cagtctcaaa aaaggtgatg agatcatcgg
      421 taaccgcgtc cgcgtcaaga tcgtcaagaa caaggtcgcg gcaccgttcc ggcaggcgga
      481 attcgatatc ctcttttcgg agggaatctc gcgttcctca aacctccttg aactcggcgt
      541 catgtaccag gtcatcgaaa aatcggggac ctggcttgct tacaatggcg aaaaactcgg
      601 gcagagcaag gacgccgccc ggatgttctt gaaggaaaat ccgaaacttc tcgaggagat
      661 cgaatccagg atcatcgaga aagtcgccgc cggcgaaacg gccaccgtcg gagtggcgga
      721 ctagaccgct ttcccgcgtc ccatggcaga gccgccggat gaaaagtcaa aacaggctct
      781 gatcacagcc ctgaggctgc tggccgcaag tcccaagagc cagcgtgaac tccggacaaa
      841 gctcgaggcc aaaggctatc ccgtcatggc cgttcagttc gcgatgaatg agttgagcgg
      901 gcagggcgtc ctggacgaca aagcttacgc gaaaaacctg acgacgaggc ttgtgcaggg
      961 aagcgccgcg ggacggcgca gggtcgcctt cgaactcaag catcacggcg tcccggagaa
     1021 ggttcagagg gagattctgg ggtccctcac tgaatcgaat gaacgcgcgc gggccctgga
     1081 gctggcccgt tccaagtggc cgagcctctc gaaactggaa ccggccaaga gaaaaaagaa
     1141 actttttgat cacctgataa ggaaaggcta cgattatcat gttgtccgcg acgtacttga
     1201 aaccgtcgcc agggacaccc cagatgacga tccgacatta tgaaagacca atacacagct
     1261 gacgccatcc gaaaagccta tctcgatttc ttcgcatcca ggggacacac cgtcacccca
     1321 agtgactccc ttgtcccgca gaacgaccca tcgctccttt ttaccggcgc cggaatgaac
     1381 caattcaagg aatacttcct gggggtcaaa aaggacctga aacgggccgt atcaagccaa
     1441 aaatgcctca ggacgggcga tctcgacgaa gtcggccgga cgccttacca tcactccttc
     1501 ttcgaaatgc tcggcaattt ctcgttcggg gactatttca agaaggaagc catcatctgg
     1561 gcctgggaat tcctgaccgc cattctgtcc atcccgaaag aacggcttcg catcacggtc
     1621 catgagaacg atcaggaggc ctgcgacctc tggcgcaagg agatcggcct tcccgaagag
     1681 tggatttaca aatgcggcga caagtccaac ttctggcccg cgaacgcacc caaggatggt
     1741 ccgaacggcc cctgcggccc gtgctccgaa atctattatg acctgaaccc ctcgatcacc
     1801 ggcacgccgg tcgacgactc gggaaaatac gcggagatct ggaacctcgt cttcacacag
     1861 tatgaccgac aagagggcgg aaagctcgtg cccctcaagg ccaggaacat cgacaccggc
     1921 atgggccttg aa
//
LOCUS       DAOOBX010000184         1928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_65440_length_1928_cov_2.950347, whole genome shotgun sequence.
ACCESSION   DAOOBX010000184 DAOOBX010000000
VERSION     DAOOBX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65440_length_1928_cov_2.950347"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..>1928)
                     /locus_tag="PLY30_03220"
     CDS             complement(150..>1928)
                     /locus_tag="PLY30_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW65149.1"
                     /translation="PYMRPIPIGASAAMMFSMVIAFVATPWAAYQILGRAFAKGKLKA
                     HHEGEREDFLTRLYRSYMNPLIRNAKVRHIFLWALGGLLLASMALVPLKLVRMKMLPF
                     DNKNEFQVILNMPDGTAIEKTREVITEIADYLVTVPEVKDVELYAGCAAPYNFNGLVR
                     HYFLRSRPHQADLQVNLAGKHDRKRQSHDIAKAVRPRIHEIVRTHGGHVQVAEVPPGP
                     PVLSTLVLEIYGPSREGQNGLAREIERLLASSEGVTDIDTYVQAPEKLARLRVNTEKA
                     SLNGIPVDQVAEAVATAIGGKTVDLAHLSEEYEPVEILLRLPREKRATLDSVLNMTLL
                     SRYGNPIPVRELVDIESGTKDLATYHKNLMRVSYVLADVAGRFESPAYAILKLKKDIA
                     KIPAPENHEGQKTLDQLFNGQPRSADRWTVKWDGEWQITFEVFRDLGIAFAFVLLLIY
                     ALVVGWFQSFKTPWIIMLPIPLTLIGILPAHWLGGVFFTATSMIGMIAGAGIVVRNSI
                     ILVDFIELQRAAGMPLEEAVVDAGAKRFRPMLLTAAAVVVGAAVILFDPIFQGLAVSL
                     MAGEIASTVLSRTAVPVFYYMMAEKT"
BASE COUNT          381 a          604 c          578 g          365 t
ORIGIN      
        1 caggaccgcg agcacgacgc tcatcagaat gaagcttccc gcgataatac ggatgtattt
       61 ttcaatagtc atttcagaac ctccgggtgc ggggtacaag gcggaaaaac cggacctttg
      121 cctccggact ccgcaccccg tacctcgtac taggtttttt ccgccatcat ataatagaac
      181 accggcaccg ccgtgcggga caggaccgtc gaagcgatct cccccgccat gagcgacacg
      241 gcaagtccct ggaagatggg atcgaacagg atgaccgcgg ctccgaccac gacggccgcc
      301 gccgtaagga gcatcggacg gaaccgcttc gcgcccgcgt cgacgacggc ctcctcgagc
      361 ggcatcccgg ccgcgcgctg gagttcgatg aaatcgacga ggatgatgga gttccggacc
      421 acgatccccg cgcccgcgat cataccgatc atcgaggtcg ccgtaaagaa aacgccgccg
      481 agccaatgcg cgggcaggat gccgatcagc gtcagcggaa tcgggagcat gatgatccac
      541 ggcgtcttga acgactggaa ccagccgacc acgagcgcgt agatgaggag caggacgaac
      601 gcgaacgcga tcccgaggtc gcggaacacc tcgaaggtaa tctgccactc tccgtcccac
      661 ttcacggtcc agcggtccgc gctgcggggc tggccgttaa agagctggtc gagcgtcttc
      721 tgcccctcgt ggttctcggg ggccgggatc ttcgcgatgt cttttttgag cttcagaatc
      781 gcgtaggccg gactctcgaa ccggcccgcc acgtccgcga gcacgtacga gacacgcatc
      841 aggttcttgt ggtaagtcgc aaggtccttc gtgccgctct cgatatcgac cagctctctc
      901 acggggatcg gattgccgta ccgtgagagc aacgtcatgt tgaggacact gtcgagcgtc
      961 gcgcgttttt ccctggggag cctgaggaga atctcgaccg gttcatattc ctcggacaga
     1021 tgggcaaggt ccaccgtttt tccgccgatc gccgtcgcga ccgcttccgc gacctggtca
     1081 acggggatgc cgttcaggga ggccttttcg gtgttcaccc gcaagcgggc gagtttttcg
     1141 ggagcctgga cgtaagtatc gatatccgtc acgccttcac tcgaggcaag cagccgttca
     1201 atctcccgcg cgagcccgtt ctgcccttcg cgcgacgggc cgtaaatctc gagcacaagc
     1261 gtcgagagga ccggcgggcc gggcggaact tccgcgacct ggacatggcc gccgtgggtc
     1321 cggacgatct catggatcct cggacggacc gccttcgcga tgtcatgact ctggcgtttc
     1381 cggtcgtgtt tcccggcgag gttcacctgg aggtcggcct ggtggggtct ggaacgcagg
     1441 aagtaatgac gcacgagccc gttgaaatta taaggcgccg cgcacccggc gtagagttcg
     1501 acatccttca cctccggcac cgtcacgagg taatcggcga tctccgtgat tacctcccgg
     1561 gtcttttcga tcgccgtgcc gtccggcatg ttcaggatga cctgaaactc gttcttgtta
     1621 tcgaacggga gcatcttcat ccggaccagt ttcagcggca caagcgccat cgacgcaagc
     1681 aacagaccgc cgagagccca aagaaaaata tgccgcacct tcgcgttacg gatcagcggg
     1741 ttcatgtagg agcggtaaag acgggtcagg aaatcctccc tttcgccctc gtgatgcgcc
     1801 ttgagcttcc ctttcgcgaa cgcgcggccg agaatctggt aagccgccca cggtgtcgcc
     1861 acgaacgcga tgaccatcga gaacatcatt gccgcgctcg ccccgatcgg gatcggtctc
     1921 atgtaagg
//
LOCUS       DAOOBX010000185         1925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_65578_length_1925_cov_3.643316, whole genome shotgun sequence.
ACCESSION   DAOOBX010000185 DAOOBX010000000
VERSION     DAOOBX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65578_length_1925_cov_3.643316"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..92
                     /locus_tag="PLY30_03225"
     CDS             <1..92
                     /locus_tag="PLY30_03225"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
                     /protein_id="HPW65150.1"
                     /translation="PTWGVQFHPESVMTKDGMRLLKNFLDLSK"
     gene            89..1132
                     /gene="trpD"
                     /locus_tag="PLY30_03230"
     CDS             89..1132
                     /gene="trpD"
                     /locus_tag="PLY30_03230"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPW65151.1"
                     /translation="MSKGTRTSTPRWFNKAVNRLMAGRSLNLAEARRSFTWIFTERIP
                     FEKSKSFLLLLSRKGETADELLGCVQALQTLEKLHKTRIPGVMDTCGTGGDSRRTLNI
                     STLVALVIAGAGGKIAKHGNRAISSKSGSSDLMEAFGVKLSASRKRMILATRRSGLGY
                     FHAPYHHPVFSRMQALRRSLKRPTILNLLGPLVNPLRLDYQLVGVGDKRLLPLYAKVL
                     AKLGRKNALVCHSQDGMDEISTSTPTTAAWVTANKVRMDLIRPKTLGLKAAKPKDLSV
                     GSVEESKQRAQDILTGQDQGPARDTIVLNAAYGLLICQKARTVREGLALAERSINSGS
                     ALRVLRQLKDWSN"
     gene            1134..>1925
                     /gene="trpC"
                     /locus_tag="PLY30_03235"
     CDS             1134..>1925
                     /gene="trpC"
                     /locus_tag="PLY30_03235"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013553760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPW65152.1"
                     /translation="MILNDILLHKRKDLEELKQRMPLSRLQRIVETAPRPKARSFLHA
                     ITSGKQLNVIAEIKKASPSHGMIREDFQPLRIANLYEFGGASAISVLTEPHFFKGRPS
                     YLKTVRQVTSLPLLRKDFILEKYQIYETAILEADAFLLIASILTEEELKEFIALGLSL
                     GMDALVEIHAEPELKKAVDAGAKIIGINNRNLKTLTVDPDLATNLIPRIPKGIPVVVE
                     SGLSTYDELMMYKSLGANAFLVGTTLMKAPDIVPALQKLLGKKGPG"
BASE COUNT          433 a          623 c          526 g          343 t
ORIGIN      
        1 atccgacctg gggtgtccag ttccatccgg aatccgtcat gacaaaagac ggcatgcgtc
       61 ttctcaagaa ctttctggac ctctccaaat gagcaaggga acccggacct caaccccgcg
      121 ctggttcaac aaagcggtga accgtctcat ggccggccgt tccctgaacc tcgccgaggc
      181 gcggcgatca ttcacctgga ttttcacgga acggattccg ttcgagaaat ccaagagctt
      241 cctgctcctg ctcagccgca agggggagac cgccgatgag cttcttggtt gcgtccaggc
      301 gttgcagact cttgaaaaac tccacaagac caggattccc ggcgtcatgg acacctgcgg
      361 gacaggaggc gacagccgcc gcacactcaa catttcgacc ctcgttgccc tggtcatcgc
      421 aggcgccggc ggaaagatcg caaaacacgg taaccgcgcc atttcctcga agtccggaag
      481 ttcggacctg atggaggcct tcggcgtcaa actttccgcc tcgcgcaaac gaatgatcct
      541 cgcgacccgt cgatcgggac tcggctattt tcacgccccg taccaccatc ccgttttttc
      601 aagaatgcag gccttgcgcc gctcactgaa gcggcccacg atcctcaacc tgctcggacc
      661 gctggtcaat cctctgcggc tggattatca gcttgtcggc gtcggagaca agcgcctgct
      721 tccgctttat gccaaggtcc tggcgaagct tggccggaag aacgccctcg tctgtcacag
      781 ccaggacggc atggacgaaa tctccacgtc cacaccgacc acggccgcgt gggtcacggc
      841 gaacaaggtc cgcatggact tgatccgccc gaaaacactg ggcctcaagg ccgccaaacc
      901 caaagacctg tcggtcgggt ccgtggagga aagcaaacag cgggcgcagg acatcctgac
      961 cggccaggac cagggaccgg cgcgggacac gatcgtcctg aacgccgcct acggactttt
     1021 gatctgccaa aaggccagga ccgtccggga ggggctcgcg ctggcggaac gatcgatcaa
     1081 ctccggcagc gccttgcggg ttctgcgcca actgaaggac tggtcgaact gatatgatcc
     1141 tgaatgatat cctgcttcac aagcgaaagg accttgagga gctgaagcag cgcatgccgc
     1201 tatcgcggct ccagcgcatc gttgagaccg cgccccggcc gaaggcacga tccttcctgc
     1261 acgcgatcac atcggggaaa caactcaacg tcatcgcgga gatcaaaaag gcctcgccct
     1321 cgcatggcat gatccgcgag gattttcagc ccctgcgtat cgcgaacctc tacgagttcg
     1381 gaggcgccag cgcgatttcg gtcctgaccg aacctcactt tttcaaaggt cgccccagct
     1441 atctgaaaac agtccgccag gtcacaagcc ttccgctcct gcgaaaggat ttcatcctcg
     1501 aaaaatacca gatttacgag accgcgatcc tcgaagccga cgcgttcctc ctgatcgctt
     1561 cgatcctgac cgaagaggaa ctcaaggagt tcattgcgct cggcctttcc ctcgggatgg
     1621 acgccctggt cgaaatccac gccgaaccgg agctcaagaa ggccgtcgac gcgggagcga
     1681 agatcatcgg catcaacaac cggaacctca aaacgctcac ggtcgatccc gacctcgcga
     1741 ccaacctcat accccggatt ccgaagggga ttcccgtcgt cgtggaaagc ggactcagca
     1801 cgtatgacga actcatgatg tacaagagcc tcggcgccaa cgcgtttctc gtggggacaa
     1861 ccctgatgaa ggcgccggac atcgtgcccg cactccaaaa actgctgggt aaaaaagggc
     1921 cgggt
//
LOCUS       DAOOBX010000186         1913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_66106_length_1913_cov_2.983315, whole genome shotgun sequence.
ACCESSION   DAOOBX010000186 DAOOBX010000000
VERSION     DAOOBX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66106_length_1913_cov_2.983315"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1913)
                     /locus_tag="PLY30_03240"
     CDS             complement(<1..>1913)
                     /locus_tag="PLY30_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65153.1"
                     /translation="VSLFTLICFVTTTIAWGMPALDTEPGIRSSEIRSLTLPDHIVIP
                     PEIGSIRETCYAAGPASPSSGLIVHIEEAHGQPEGQENIARILEYLREYYNLDTVYLE
                     GVWQELEPEALRFFDDSELNRELLQELNRVGLIGGVELFLSAEYGDRGTEPASTGLEP
                     YSVSRAQVPGRVHGVEDLPLYKKNLEQFRAVYAAKPRTDAFLRDQRQKIADDASRTLN
                     PECKTFFREWAFRTEVADQPFSGLDRMAGAAAKYLDLDLTDPYEQYDWPQLVRFFRLQ
                     QLEKPGHGVQRTEHGVDETKLREWVSGKGLKPDSCEFINDLKRRAPHSAPRTTSDFRT
                     AVERFYESASPLGFRFEDYPVLAREIGSAILSSELRTPELFEEVERLNDRILEKLAGK
                     PEEKALVERYREYRILKKLFSLELTSEEYQKSVRGAEPGVKPADGSPVRAALNQARAF
                     YETAEAREQALLANMIARMKAMKQPNAVLVTGGYHSTGVTEFLKKAGISCVRVTPRIT
                     KPGKPGAYLDAMLLKDRTSSPGPERQFRSSNVRPPSFASGYHLLRQDLGSATADRLLG
                     MLREALHVISSRRKTPVGELIASLRSPALAAHGLTRDPRRPARLKPLEGRAEARLRDE
                     DVKPFLSVAEAQR"
BASE COUNT          368 a          567 c          568 g          410 t
ORIGIN      
        1 cccgttgagc ctccgcgaca ctcaggaacg gtttcacgtc ttcgtcccga agcctcgcct
       61 cggcgcgacc ttccaacggt ttcaaccgcg cgggccgacg agggtcccgc gtaagaccgt
      121 gggccgccag ggccggagac ctgagggaag cgatcaactc accgacaggc gtttttctcc
      181 ggctgctgat cacgtgaagg gcttcccgga gcattccaag aaggcgatca gcggtggcgg
      241 acccaaggtc ttgacgcagg agatgatatc cggacgcgaa cgaaggaggg cggacattgg
      301 acgaccggaa ttgtcgttcc ggccccggag acgaggtccg gtctttaagg agcatcgcat
      361 ccaggtaggc gccgggtttg ccgggtttcg tgatgcgtgg cgtgacccgc acgcaggaga
      421 tcccggcctt tttcaggaat tccgtaacgc cggtggagtg gtaaccgccg gtaacgagta
      481 cggcgttcgg ctgtttcatt gccttcatcc gcgcgatcat gttcgcgagc agggcttgct
      541 cgcgggcttc agcggtttcg taaaacgccc gggcctggtt gagcgccgcc cgcacggggc
      601 ttccgtccgc cggttttact ccgggctccg caccccgcac tgacttttga tattcctcac
      661 tcgtcaattc caggctgaaa agcttcttca gtatccggta ctccctgtac cgctcgacca
      721 gcgccttctc ttcgggcttg ccggcgagtt tttcaaggat gcggtcgttc agcctttcga
      781 cctcttcgaa aagttcgggg gtccggagtt cggaggaaag gatcgcgctg ccgatctccc
      841 tggccaggac gggatagtct tcgaatctga aaccgagcgg tgaggcggat tcataaaaac
      901 gctccacggc cgttcggaaa tcagatgtag tgcggggcgc ggagtgcggg gcgcggcgtt
      961 tcagatcatt gataaattcg caagagtccg gtttgagacc tttcccgctc acccattcac
     1021 gcagttttgt ttcatccacc ccgtgctccg tacgttgtac gccgtgcccg ggtttttcaa
     1081 gctgttgaag tctgaaaaat ctgacgagtt gcggccagtc atactgttcg taaggatccg
     1141 tgagatccag gtccagatat ttcgcggcgg cgccggccat tcgatcgaga cccgagaacg
     1201 gctgatccgc aacctcggtc cggaaagccc attccctgaa gaaggttttg cattcggggt
     1261 tcagggttcg ggaagcgtca tcggcgatct tctgccgctg atccctgagg aacgcatcgg
     1321 tccgcggttt ggcggcatag accgcccgga actgctcgag attctttttg taaagcggaa
     1381 gatcttccac gccatggaca cgccccggaa cctgggcacg ggatacggag tacggctcaa
     1441 gacccgttga cgcaggctcc gtgcctcggt ctccgtactc tgcactcaaa aaaagctcga
     1501 cgccgccgat cagcccgacc cggttcaatt cctgaagaag ctcccgattg agctcactgt
     1561 catcaaagaa ccggagcgcc tcgggctcca gctcttgcca gacaccttcg aggtatactg
     1621 tatcaagatt gtaatattcg cggagatatt cgaggattct ggcgatgttc tcctgcccct
     1681 ccggttgccc gtgggcctcc tcaatatgga caatgagtcc ggaagaagga ctcgcgggac
     1741 cggccgcgta gcaagtttcc cgtattgaac cgatctccgg ggggatgacg atgtgatccg
     1801 gaagcgtcag cgaccggatc tccgaactcc ttattcccgg ctccgtatcg agtgcgggca
     1861 ttccccacgc aatcgtggtc gtgacaaagc agatcagcgt gaacagactg acc
//
LOCUS       DAOOBX010000187         1908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_66358_length_1908_cov_2.845656, whole genome shotgun sequence.
ACCESSION   DAOOBX010000187 DAOOBX010000000
VERSION     DAOOBX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66358_length_1908_cov_2.845656"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLY30_03245"
     CDS             complement(<1..265)
                     /locus_tag="PLY30_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65154.1"
                     /translation="MSRIDFEEESRMHDFCYNRKGELCCENVPLRKILEKTGTPAYIY
                     SSKTLTDHLAKLQRAFRSVRPLICYSVKANSNLAVLREFAGLGS"
     gene            complement(372..1751)
                     /gene="argH"
                     /locus_tag="PLY30_03250"
     CDS             complement(372..1751)
                     /gene="argH"
                     /locus_tag="PLY30_03250"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPW65155.1"
                     /translation="MSKKLWGGRFKKEMHPALRCFSYSLAVDKELFDAELAVNAAWAK
                     MLARTAIITQAESKAILKGLARIEKDLEGKDIARYVAEVEDIHTLIQSELEKRSGAVA
                     KKLHTGRSRNDLVVTSTRVCLREKIAVIRYETSRLQKALVAAAEKAQGAIVPGMTHLR
                     KAQPVLLAHHLLAYVEMLEEDKARLADTLKRVNILPIGSAALAGSALPIDQKFLAKEL
                     GFEKIARNSMTAVSDRGFLAEFLANLSILWMHLSRLAEDFILWNSEAFGYVELDDAFA
                     TGSSLMPQKKNPDVFELVRGRAGVIFGHLQSILTVQKGLPLSYNRDLQEDKPGAFDAV
                     HKTTIALEVLALTVASATWQKKAMAKAVADDTLFATDILEYLVRKKVPFSEAHSTVGQ
                     AVRFAAESGKGLRELSLREWKRFSPGIGADIYDLFDPETSVRGKKTIGSTHPLKVRQQ
                     MDYWTRLLA"
BASE COUNT          346 a          578 c          573 g          411 t
ORIGIN      
        1 cgctgccaag acccgcgaac tcccggagaa cggcaaggtt cgaattggcc ttcacggaat
       61 aacagatcag gggccgcacg gagcggaacg cgcgctggag cttcgcgagg tggtcggtga
      121 gcgtcttcga gctgtagata tacgcgggcg tgccggtctt ctccaggatc ttcctgagcg
      181 ggacattctc gcagcagagc tcgcccttcc ggttatagca gaaatcgtgc atccgggatt
      241 cttcttcgaa atcgattcgt gacataatcg ttacctcgat tttccaggat agttaactcc
      301 cctctttcct gggaggggcc gggggagatt tcagattccc ctggattccc ccttggccga
      361 agggggaacg ttcaagcaag cagtctcgtc caatagtcca tctgctgacg gaccttgagg
      421 ggatgcgtgc tgccgatggt cttcttgcca cggaccgaag tctcggggtc gaagagatcg
      481 taaatatccg cgccgatccc gggggagaac ctcttccact ccctgagcga aagttcccgc
      541 agccccttgc ccgactccgc ggcgaagcgc acggcctggc cgacggtcga gtgggcctcc
      601 gagaacggga ccttcttgcg gacgaggtac tcaaggatgt ccgtggcgaa gagcgtgtcg
      661 tccgcgaccg ctttcgccat cgcctttttc tgccaggttg cggacgcgac cgtgagtgcg
      721 agcacttcaa gggcgatcgt cgtcttgtgg accgcatcga atgcgccggg cttatcctcc
      781 tggaggtccc ggttataaga aagcggcagt cccttctgga cggtcaggat cgactgcaga
      841 tgcccgaaga tcaccccggc cctgccccgc acgagctcaa agacatcagg gttcttcttc
      901 tgcggcatca ggctggagcc cgtcgcgaac gcgtcgtcga gctcgacata cccgaatgcc
      961 tccgagttcc agaggatgaa atcctcggcg agcctcgaaa gatgcatcca gaggatcgaa
     1021 aggttcgcca gaaattccgc aaggaacccc cggtcgctca cggcggtcat gctgtttcgg
     1081 gcgatttttt cgaagccgag ctccttcgcg aggaattttt gatcgatcgg aagcgcggaa
     1141 cccgcgagcg ccgcggaacc gatcgggagg atattgacgc gcttcaaggt gtcggcaagt
     1201 cgggccttgt cctcctcgag catctcgaca taggcgagga ggtgatgcgc gagcagcacc
     1261 ggctgggcct tcctcagatg cgtcatgccg gggacaatgg ccccctgggc tttttccgcc
     1321 gcggcgacga gcgccttctg gagacgcgag gtttcatacc gtatcaccgc gatcttttcc
     1381 cggaggcaga cccgggtgga cgtgacgacg aggtcgttcc ggctgcggcc cgtgtggagt
     1441 ttcttcgcga ccgcgcctga gcgcttttcg agttcgcttt ggatcagggt gtgaatgtct
     1501 tcgacctccg cgacatagcg ggcgatgtct tttccctcga ggtccttctc gatccgcgcg
     1561 aggcctttca ggatcgcctt cgattcggcc tgtgtgatga tcgcggtccg cgcgagcatc
     1621 ttcgcccacg ccgcgtttac ggcgagttcc gcgtcaaaga gctctttgtc gacggcgagc
     1681 gaatagctga agcatctcaa cgccggatgc atttccttct tgaaccgacc gccccagagt
     1741 tttttggaca tatttacccc cagcttggag cccagcgacg agagccaaga gctgttccag
     1801 gctcttcgtt tttcgccctt cgcgcttagc ttttatacat tccctcgtac ggaaggcccc
     1861 agagtttcat gaaccccgtc gcggccgtcc ggtcgaagag atcgccct
//
LOCUS       DAOOBX010000188         1891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_67152_length_1891_cov_3.074074, whole genome shotgun sequence.
ACCESSION   DAOOBX010000188 DAOOBX010000000
VERSION     DAOOBX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67152_length_1891_cov_3.074074"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1891
                     /gene="pnp"
                     /locus_tag="PLY30_03255"
     CDS             <1..>1891
                     /gene="pnp"
                     /locus_tag="PLY30_03255"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPW65156.1"
                     /translation="IIIETGHLARQAHGAVTVQCGGTVVFVAVVAGAESDQIRDFFPL
                     TVDYRERTYAAGKIPGGYFKRESKPTEKETLTSRLIDRPIRPLFPEGYQTEVQISAIT
                     LSFDGENPTEILAMIGASAALLISDIPYTVPLSAVRVGMIDGKLIINPTQQELDKGDL
                     DLVIAGTPEKVVMIEAGSKEISEEKMFEAIKFGHEAIKQTAQLQLELMKKAGKPKKAV
                     KTLDIPAEITQKVNEVAADEFQKAFGLKTKEEQEGFFKQLYEKVMAQIDTKAEGFNEI
                     YVKIAFSRLEHQKVREYMLATGKRADGRGERDIRQIKCEAGMLPRTHGSSLFTRGQTQ
                     SLSVVTLGTGDDEQRIDALEGEQTRRFMLHYNFPSYSVGECKPNRGPGRREIGHGALA
                     ARALEPVIPFDESFPYTVRLVSEIMESNGSSSMATVCGGTLALMDAGVPIKAPVAGIA
                     IGLVTDKNRWKVLTDIAGIEDHLGDMDFKAAGTRKGLTALQMDLKIDGITDEILKEAF
                     DHAKQGRFKILDMIQATISAPKELSMFAPRITTLRINPEKIGALIGPGGKNIRKIVEE
                     TGAKIDIEDDGRVFVAAVDGAVSAAAIARIQGCTAEAEVGKIYENATVQKLMPFGAFC
                     EILPDL"
BASE COUNT          421 a          552 c          586 g          332 t
ORIGIN      
        1 catcattatc gagacgggcc atttggcccg tcaggcccac ggcgccgtca ccgttcagtg
       61 cggcgggacc gtcgtgttcg tggccgtggt agccggggcc gaatcggacc aaatccgcga
      121 tttttttccg ctcacggtcg attaccgcga acgcacctat gccgccggca agattccggg
      181 cggttacttc aaacgcgaga gcaaacccac cgagaaagag accctgacgt cgcgcctgat
      241 cgaccggccg atccgtccgc tctttccgga aggataccag accgaagtcc agatcagcgc
      301 gatcacactc tcgtttgacg gtgagaatcc gaccgagatt ctcgcgatga tcggcgcttc
      361 cgcggccctt ctcatttcgg atatcccgta taccgtgccg cttagcgcgg tccgcgtcgg
      421 gatgatcgat ggcaagctca tcatcaatcc cacgcagcag gagctcgata agggtgacct
      481 cgacctcgtg atcgcgggaa cgcccgagaa ggtcgtcatg atcgaagcgg gctcgaaaga
      541 gatctccgaa gagaaaatgt tcgaggcgat caaattcggg catgaggcga tcaagcagac
      601 ggcgcaactt cagcttgagc tcatgaaaaa ggccggcaaa cccaaaaagg ccgtgaagac
      661 gctcgacatc ccggcggaga tcacacagaa ggtgaatgag gtcgccgcag acgaattcca
      721 gaaggcgttt gggctcaaga cgaaagaaga gcaggaaggt tttttcaaac agctttacga
      781 aaaggtaatg gcccagatcg acacgaaggc tgaagggttc aacgaaatct acgtgaagat
      841 tgccttctcg cgtcttgagc accagaaggt ccgcgagtac atgctcgcga ccgggaagcg
      901 ggcggatggc cgcggcgagc gggacatccg tcagatcaag tgcgaagcgg gcatgctgcc
      961 ccgcacgcac gggtcctcgc ttttcacgcg cggtcagacc cagagcctga gcgttgtgac
     1021 gctcggcacg ggcgatgacg agcagcggat cgacgccctc gaaggcgagc agaccaggcg
     1081 cttcatgctt cactacaatt tcccgtccta cagcgtcggc gaatgcaagc cgaaccgcgg
     1141 tcccggacgg cgcgagatcg gacacggtgc cctggcggcc cgcgcgctcg aacccgtgat
     1201 tccctttgac gagagtttcc cttatacggt gcgtcttgtg tccgagatca tggaatcgaa
     1261 cggttccagc tcgatggcga ccgtctgcgg cggaacactc gcgttgatgg atgccggcgt
     1321 gccgatcaaa gcgccggtgg ccggcatcgc gatcggcctc gtgacggaca agaaccgctg
     1381 gaaggtcctg actgacatcg cggggatcga agaccatctt ggcgatatgg atttcaaggc
     1441 agccgggacc cgcaagggtc tcaccgcgct ccagatggac ctcaagatcg acggtatcac
     1501 ggacgagatt ctgaaagagg cttttgatca cgcgaagcag ggccgcttca agatcctcga
     1561 catgatccag gcgacgatca gcgctccgaa ggagctttcg atgttcgcgc cgcgtatcac
     1621 gacgctccgc atcaaccccg agaagatcgg cgcgttaatc ggacccggcg gcaaaaatat
     1681 ccggaagatc gtcgaagaga ccggcgccaa gatcgacatc gaggatgatg ggcgcgtgtt
     1741 cgttgccgcg gtggacggtg cggtgtcggc ggccgcgatc gcccgtatcc agggctgcac
     1801 ggcggaagcc gaggtcggta aaatttacga gaacgccacg gtgcagaaac ttatgccgtt
     1861 tggcgcgttc tgcgagatcc ttccggacct g
//
LOCUS       DAOOBX010000189         1878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_67772_length_1878_cov_2.424575, whole genome shotgun sequence.
ACCESSION   DAOOBX010000189 DAOOBX010000000
VERSION     DAOOBX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67772_length_1878_cov_2.424575"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..393)
                     /locus_tag="PLY30_03260"
     CDS             complement(16..393)
                     /locus_tag="PLY30_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65157.1"
                     /translation="MKKLPLFVMVLLSVLALGSVIAHAELTPPVNGESDDAVVGVATD
                     EDPGDSSVVDLSDIDTTQLFNTPDEDTLAGIATDEEDPGDSGVVDPSDSTTGYVDSPS
                     FDNAAPSGMAPGEPIDGKNPDFS"
     gene            complement(544..>1878)
                     /locus_tag="PLY30_03265"
     CDS             complement(544..>1878)
                     /locus_tag="PLY30_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW65158.1"
                     /translation="REKEAIYAKPDFSDADGLRVADLEHQFSEMHGWDAEAEAAKLLS
                     DLGIPDKLHDLQMKQLEANQKVRVLLAQALFGNPDILLLDEPTNHLDVETCLWLEDFL
                     YDFKNTAIVVSHDRHFLDRVCTHIADIDFGKIQLYAGNYSFWYESSQLALRQRSESNR
                     SIEEKRKELQTFIARFSANASKSRQATSRKKLLEKLTLEDIKPSSRRYPNIIFKPERE
                     AGNDLLEIDGLECSIDGELMFRNLDLRIDKGDKVAFVGPLDRTKSTLFQVLAGEIAPD
                     RGSFKWGASTSRAYFPRENRQYFETDLDLVDWLRQFSREKDENFVRGFLGRMLFSGEE
                     SLKKFSVLSGGERVRCMLARMMLSGANVLIMDEPTNHLDLESITALNQGLEKFPGTVL
                     FSSHDHQFVQTVANRIIELTPLGIIDRRETALDTFLTDPEIRAKRATLYPAA"
BASE COUNT          368 a          499 c          586 g          425 t
ORIGIN      
        1 tggttttttg tccgactaag aaaaatcggg atttttcccg tcgatcggtt cgccaggggc
       61 catgcccgac ggggctgcat tatcgaagct cggcgaatcg acatatcctg tcgtggaatc
      121 gctcggatca accacaccgc tgtcacccgg atcttcttcg tccgtggcaa tcccggcaag
      181 ggtgtcttcg tcgggggtgt tgaacagctg ggtggtatcg atatcgctaa ggtcgacaac
      241 gctgctatcg cccggatctt cgtccgtcgc gacgccgacc acggcgtcat cgctttcccc
      301 gttgacgggc ggggtaagct ccgcgtgtgc gattaccgaa cccagggcaa gaacggacag
      361 cagaaccatc acaaaaagcg gcaacttttt catctgaatc tccttcatgg gtgagatatt
      421 gacgacccgg ggattctact ccttcggatg atctcttgca aaggtcaaat tgacgaccgc
      481 agaaccccgc attcggtttt ttttgcgatg atgaagtatt gtttcggaga ggaaggatgc
      541 cgttcaggca gccggataca acgtagcgcg tttggccctg atctcgggat cggtcaggaa
      601 tgtgtccagt gcggtttccc gacggtcgat gatgccaagt ggcgtcagtt cgatgatgcg
      661 gttcgcgacg gtctggacaa actgatggtc gtgggaggag aaaaggaccg ttcccgggaa
      721 tttctcgagg ccctggttca gggcggtgat cgattccagg tccaggtggt tcgtgggctc
      781 gtccataatc aggacgttcg cgcccgagag catcatgcgc gcgagcatgc agcggactcg
      841 ctctccgccg gaaagtacgg agaacttctt gagggactcc tcgccggaaa aaagcatccg
      901 gccgagaaag cctcggacga agttctcgtc tttttccctg gagaactggc ggagccagtc
      961 gacgaggtca aggtccgtct cgaaatactg gcggttctcg cgcgggaagt aggcgcgcga
     1021 ggtggatgcc ccccatttga aacttccgcg gtcgggcgcg atttcccctg ccaggacctg
     1081 gaagagcgtg gatttggtgc ggtcaagggg accgacgaac gctaccttgt cgcccttgtc
     1141 gatccggaga tcgagattcc ggaacatgag ctccccgtca atcgaacatt cgaggccgtc
     1201 gatctcaagg aggtcgttcc ccgcttcccg ctcgggtttg aagatgatgt tgggataacg
     1261 gcgcgacgaa ggtttgatgt cctcgagggt aagtttttcg aggagcttct tgcgggaggt
     1321 cgcctgacgc gatttcgaag cgttcgcgct gaaccgggcg ataaaggtct ggagttcctt
     1381 gcgcttctcc tcgatgctcc ggtttgattc cgaccgctgc ctcagggcga gctggctgga
     1441 ttcgtaccag aacgaatagt tccccgcgta gagctggatt tttccgaaat cgatgtcagc
     1501 gatatgagtg cagacccggt cgaggaaatg ccggtcgtga gaaacgacaa tggcggtgtt
     1561 tttgaaatca tagaggaaat cctcgagcca cagacaggtt tccacgtcga ggtggttggt
     1621 cggttcatcg aggagcagga tgtcgggatt tccgaaaagg gcttgcgcaa gaaggacgcg
     1681 gaccttctgg ttggcctcga gctgcttcat ctggaggtcg tggagcttgt cgggaatccc
     1741 gaggtcgctc aggagcttgg cggcctccgc ctcggcgtcc cagccatgca tttccgagaa
     1801 ctggtgctcg aggtccgcga cgcgcaaccc gtccgcgtcc gaaaagtccg gttttgcgta
     1861 gatcgcctct ttctcgcg
//
LOCUS       DAOOBX010000190         1875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_67899_length_1875_cov_1.802747, whole genome shotgun sequence.
ACCESSION   DAOOBX010000190 DAOOBX010000000
VERSION     DAOOBX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67899_length_1875_cov_1.802747"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLY30_03270"
     CDS             <1..736
                     /locus_tag="PLY30_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008314472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPW65159.1"
                     /translation="VSSGKGGVGKSTAAVNLALALKKLGARTGLMDGDIYGPNIPIML
                     GIPSDRRTEPGGSGKILPIEAHGIRAVSIGFFANPEQPMIWRGPLLHKTVQEFLHRVE
                     WGELDFLVVDLPPGTGDVQLSLAQWVRVSGAVIVTTPQEVALADVRRAINMFRQLEIP
                     VLGVVENMTGPVFGRGGGERVAKDFQVPFLGDIPLDAEIRECGDAGVPIVAATAGSGT
                     SERFIKIAEAIRQAFYKNDNPSKTRS"
     gene            773..1366
                     /gene="folE"
                     /locus_tag="PLY30_03275"
     CDS             773..1366
                     /gene="folE"
                     /locus_tag="PLY30_03275"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPW65160.1"
                     /translation="MKNVGTKKSEEDKFQKLVGQMLIMLGEDPTREGLQRTPLRVSRS
                     LRYLTEGYKTDPREILAHAVFKEPYDEMVIVKDIEIFSLCEHHLLPFYGKAHIAYIPN
                     GKIIGLSKIPRLVEVFSRRLQVQERLTTQIAESLMSALKPKGVAVVIEALHLCMCMRG
                     VKKSDTYTITSSMLGAFRRDARSRTEFLSLIRLKREE"
     gene            complement(1455..>1875)
                     /locus_tag="PLY30_03280"
     CDS             complement(1455..>1875)
                     /locus_tag="PLY30_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPW65161.1"
                     /translation="IGACCTVDRAALGSTRICKGVKIDNLVQIAHNVTVGPHTVISAQ
                     TGISGSCKIGAHVTMGGSVGVGDHVEIGDWAMIGAGAGFPSNKKVPGKQIYFGQPARP
                     YAEMRKQFGAQLRAAETLDDVRALKKKVAELEKKLSS"
BASE COUNT          395 a          499 c          576 g          405 t
ORIGIN      
        1 cgtttcgagc ggcaaggggg gggttgggaa atccacggcc gccgtcaatc tcgcactcgc
       61 cctgaaaaag ctgggagccc ggactggtct tatggacggt gatatctatg gccccaatat
      121 tccgatcatg ctcgggattc ccagcgacag acggaccgaa ccgggcggat cggggaagat
      181 tcttcccatc gaagcgcacg ggatcagggc cgtttcgatc ggttttttcg cgaaccccga
      241 acagccgatg atttggcgcg ggccgctgct ccacaagacg gttcaggagt ttcttcaccg
      301 ggtggagtgg ggggagctgg attttctggt cgttgatctg cctccgggaa ccggagatgt
      361 tcagttgtct ctcgcgcagt gggtcagggt ttcgggcgcc gtgattgtca cgactcccca
      421 ggaggtggcg ttggcggatg tgcggcgcgc gatcaacatg ttccgtcagc ttgagatccc
      481 ggtactcggg gtcgtggaga atatgacggg tcccgtgttc gggagaggcg gcggggaacg
      541 ggtggcgaag gactttcagg tgccgttcct gggggatatt ccgcttgatg cggagatcag
      601 agaatgcggg gatgccggag tgccgattgt cgccgcgaca gccgggagcg gaacaagtga
      661 acgctttata aagatcgccg aggcgatccg tcaggctttt tataaaaacg ataacccttc
      721 caagacccgg tcctaagacc cggtcttgaa acgggtggga gaatggatga cgatgaaaaa
      781 tgtgggcacg aaaaaatcgg aagaagacaa gttccagaaa ttagtcggac agatgcttat
      841 catgcttggg gaggacccga cgcgtgaagg attgcagcga acacccctcc gcgtttccag
      901 atcgctccgg tacttgaccg aaggatacaa gaccgacccc agggagatac tcgcgcacgc
      961 ggtttttaaa gagccttatg atgaaatggt gatcgtgaag gatatcgaaa tcttctccct
     1021 ctgcgagcat caccttcttc ccttttacgg caaggcccat atcgcgtata tcccgaacgg
     1081 aaagatcatc gggctctcca agatcccgcg gcttgtcgag gtcttttcaa gaaggcttca
     1141 ggtccaggag cgcctcacga cgcagattgc ggaatccctc atgagcgccc tgaaaccgaa
     1201 aggggtcgcg gttgtgatcg aagccctgca tctttgcatg tgcatgcgcg gtgttaaaaa
     1261 gtccgatacc tacacgatca cgagctccat gctcggcgcc tttcgcaggg atgcccgttc
     1321 ccgcactgaa ttcctgagtt tgatccggct gaaaagggaa gagtaaagac gggctcgcta
     1381 caaagacaca agtcgtgcgg ctacggcgcg gggctgatca tgccgcctgc ggtcggcggt
     1441 gtgaatcgtg cggcttagga agagagcttc ttttccagtt ccgcgacttt tttcttaagg
     1501 gcgcggacat catccagggt ttctgcggcg cggagctggg caccgaactg tttcctcatt
     1561 tcggcatacg gcctggcagg ttgaccgaaa taaatttgtt tgcccgggac ttttttattg
     1621 gaagggaagc ctgctcccgc accgatcatc gcccaatcac cgatctctac gtgatcgccg
     1681 acccctacgc ttcctcccat cgtgacgtga gctccgatct tgcagcttcc ggagatcccg
     1741 gtctgggcgg aaataaccgt gtgaggaccg accgtgacat tatgggcgat ctggacaaga
     1801 ttatcgatct tgacgccttt gcagatccgc gtggacccga gcgccgcgcg gtcgaccgtg
     1861 cagcaggctc cgatc
//
LOCUS       DAOOBX010000191         1869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_68169_length_1869_cov_4.235391, whole genome shotgun sequence.
ACCESSION   DAOOBX010000191 DAOOBX010000000
VERSION     DAOOBX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68169_length_1869_cov_4.235391"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PLY30_03285"
     CDS             complement(<1..1078)
                     /locus_tag="PLY30_03285"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPW65162.1"
                     /translation="MSYNVTTLPNGVRVLTVAMPGRDSAAVAVWVRTGARHESKRVSG
                     ISHFLEHMLFKGTKNRTTRQIKEEVEGVGGILNAFTGEEMTCYFSKLLREHYPKALDV
                     LSDMVLNATIPPDEFTREKPVILEEIKMYRDRPSHQVHDLMGELLWPDQPLGRSIAGT
                     PGSVSGLTRSDLAAYMKKEYQNANLLVAVSGNVSHAEVVERVQSIYRRSGTRKSTAFV
                     PADSSQPRLRTNFFEKKTEQTYFVLGFHALARMHPDRYKLGLLHIILGANMSSRLFEE
                     IREKRGLAYEIKSHVSAYHETGAFTVSAGVETAKTAKAASVILKELSKVTKHRVKSGE
                     LRRAKDYFMSQVYQNDRSGLRRLRG"
     gene            complement(1083..1514)
                     /gene="ruvX"
                     /locus_tag="PLY30_03290"
     CDS             complement(1083..1514)
                     /gene="ruvX"
                     /locus_tag="PLY30_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPW65163.1"
                     /translation="MAGGILGLDMGGKRIGIAVVQAGTTIALPVGMIERRNREQVLAE
                     IQKLVKSYRAEKIVVGLPVTLKGEMGIAAGKLMKEMEWFRANLAVPVIMWDERLSSKE
                     AERVLLEADLSRDRRKEVIDKLAAQRVLQCYLDHSANAQKE"
     gene            complement(1577..>1869)
                     /locus_tag="PLY30_03295"
     CDS             complement(1577..>1869)
                     /locus_tag="PLY30_03295"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPW65164.1"
                     /translation="QKSRGFVYYVSLRGVTGARKAMSSDLGPHLKKLKRLTHKPVLAG
                     FGISTPEQAAAISKISDGVIVGSAVINQLKKSVPAAEQFIAKLARAVHSGRA"
BASE COUNT          375 a          585 c          502 g          407 t
ORIGIN      
        1 gaccgcgaag acggcgaagg ccgcttcggt cattctgata gacctggctc atgaaataat
       61 ccttcgcgcg ccggagttct ccggacttca cgcggtgttt ggtgaccttc gacagttctt
      121 tcagaatgac cgaagcggcc ttcgccgtct tcgcggtctc gacgccggca gacaccgtga
      181 aagcacccgt ctcgtgataa gcggagacat gggatttgat ttcgtacgcc agcccgcgtt
      241 tttcgcggat ctcttcaaag agacggctcg acatattcgc cccgagaatg atgtgcagga
      301 gcccgagttt gtaacggtcg ggatgcattc tcgcgagagc gtgaaagccg aggacaaaat
      361 atgtctgctc ggtctttttc tcgaagaaat tcgtccgcag acgcggctgg ctgctgtccg
      421 caggcacgaa cgccgtgctt ttcctggtcc cggaccggcg gtaaatggac tggacccgct
      481 ccacgacctc cgcgtgcgaa acattaccgg aaacggcaac gaggagattc gcgttctgat
      541 attctttctt catgtaagcc gcgaggtcag accgcgtcaa cccggacacc gaccccgggg
      601 tccccgctat cgaacgtccg aggggctgat caggccacag gagctccccc atcaggtcgt
      661 ggacttgatg agacggacgg tcgcggtaca tcttgatctc ttcgaggata acaggttttt
      721 cgcgcgtgaa ctcgtccggc gggattgtcg cgttcaggac catgtcggag agcacatcga
      781 gggccttcgg gtagtgttcc ctgagaagtt tcgagaaata gcacgtcatc tcttcgcccg
      841 tgaaagcgtt caggataccg ccgaccccct caacttcctc cttgatctgg cgggtcgttc
      901 ggttcttcgt ccccttgaac agcatgtgct cgagaaaatg agaaatcccg gagacccgct
      961 tcgattcgtg acgcgctccg gtcctcaccc acacggccac tgccgcagaa tcgcgacccg
     1021 gcatcgcgac cgtcaggacc ctcacgccgt tcggtaaagt cgtaacgtta taagacacgg
     1081 atttactcct tttgcgcgtt cgcgctatga tcaagatagc actgcagcac tcgctgcgcc
     1141 gcgagtttgt cgatcacttc cttccgtcgg tcgcggctca ggtcagcctc cagcagtacc
     1201 cgctccgctt ccttcgaaga gagcctttca tcccacatga taacaggaac ggcaagattc
     1261 gccctgaacc attccatttc tttcatgagt ttcccggccg cgatccccat ctcgcccttg
     1321 agcgtcacgg gaagtccgac cacgatcttt tcggcccgat aggacttcac gagcttctga
     1381 atctcggcca ggacctgttc gcgattcctc cgttcgatca tcccgaccgg caacgcgatc
     1441 gtagtcccgg cctggaccac ggcgatcccg atccgcttcc cgcccatatc cagacccaga
     1501 ataccgccag ccataattat cgattcctca cttgagatgg agagagcccc ggaatccggc
     1561 cggtcctttt gaaaaactat gcgcggccgg aatgaacagc ccgcgcaagc tttgcgatga
     1621 actgttcagc tgccggcacg ctttttttga gctgattgat aaccgcgctg ccgacgatca
     1681 caccgtccga gatcttcgag atcgcggcgg cctgctccgg cgtcgagatc ccgaagccgg
     1741 ccaacaccgg tttgtgggtc aggcgcttga gctttttcag atgcggcccg aggtcggacg
     1801 acattgcctt gcgcgctccc gtcacaccgc gaagcgacac ataataaacg aatccccggg
     1861 acttttgaa
//
LOCUS       DAOOBX010000192         1869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_68175_length_1869_cov_3.488423, whole genome shotgun sequence.
ACCESSION   DAOOBX010000192 DAOOBX010000000
VERSION     DAOOBX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68175_length_1869_cov_3.488423"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PLY30_03300"
     CDS             <1..801
                     /locus_tag="PLY30_03300"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPW65165.1"
                     /translation="AAHIGDQAAVAVENAKLHAEIVKQETARQALKIAHEIQQNLLPD
                     LKNHRYGTDLFAKSIPAFDVGGDFYDVAVLDHGRISVILGDVSGKGVPAALYMVRAMS
                     EYRFLAPQAKDPAELLTQLNRKLSEQSFLGMFITMVCLMIDPHTGSVQYSSAGHLPVL
                     TRRAADRSTELLKGPQSVPLGLAPDAPFFLSTVKLEKHDALFLYTDGITEARNRNGKE
                     YTVERLAACVADPAPSAEAYLDRICEDLSRFSSGAEQHDDITALAVIL"
     gene            complement(852..>1869)
                     /locus_tag="PLY30_03305"
     CDS             complement(852..>1869)
                     /locus_tag="PLY30_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW65166.1"
                     /translation="TLLQLVPSSRGYLFLAGPDKQLWLESATFTPKEASAVLLGAAQA
                     AFDDGKPSAEKGLLFLPLIAGNASVGLACLERAAAFTSRETETGFNLASEFSGALKNI
                     LLFEENLKMERLAAIGQTTAMLVHEIKNILQLAKFSDEMIRMGVNEKNPKFIATGLGK
                     LEKTLKDMDGFIWEVAGLSKDYQLEPEDVDVPALLQELGEDLAERAGALSVKLDCAAE
                     KDFPVVRGDGRSLYRALLNLVKNAFEAFKDKPDAWIRVRARVTGPETYEITVEDNGCG
                     MPEEVRARLFEAFFSTKGKRGTGLGLMVVSRTVKMHGGVVTVESRPGIGTKFTLVLPR
                     FFSR"
BASE COUNT          408 a          584 c          515 g          362 t
ORIGIN      
        1 gccgcgcaca taggtgacca ggctgcggtc gcggtcgaga acgcgaaact gcatgccgaa
       61 atcgtcaaac aggagacggc caggcaggcc ctcaagatcg cccacgaaat ccagcagaat
      121 ctccttcccg acctgaagaa ccaccgttac ggcaccgatc ttttcgcgaa gtccattccg
      181 gcattcgatg tgggaggcga tttctacgac gtggcagttc tcgatcatgg caggatcagc
      241 gtgatcctgg gcgatgtgtc gggcaagggc gtaccggcag cgctttacat ggtacgcgcg
      301 atgtcggaat accggtttct cgcgccacag gccaaagacc ccgcggaact cctgacccag
      361 ctcaaccgga aactctccga gcaaagcttt ctcggcatgt tcatcacaat ggtctgtctt
      421 atgatcgacc cgcacacggg atcggtccag tactcgtccg cgggacacct tccggtcctc
      481 acccgccgcg ccgcggaccg ttccaccgag ctcctcaagg gtccccaaag cgttccgctc
      541 ggactcgcgc cggacgcgcc gttttttctc agcaccgtaa aacttgaaaa acacgacgcg
      601 ctttttcttt acacggacgg catcacggaa gccaggaacc ggaacggaaa agaatacacc
      661 gtcgaacgcc tcgcggcctg cgtcgcggat cccgcgccct ccgccgaagc ctatctcgac
      721 aggatctgcg aggacctcag ccgcttctcc agcggcgccg aacagcacga cgacatcacc
      781 gccctcgcgg tcatactcta gaccggctga aaaatcatta ttgaccggtc agctgcggaa
      841 agttcctgtt tttatcggga aaagaaccgg ggtaaaacga gggtgaattt cgtgccgatg
      901 ccaggcctgc tttcaacagt gaccacccct ccgtgcatct tgacggtgcg ggaaacgacc
      961 atgaggccga gaccggtccc gcgcttccct ttcgtggaga agaacgcctc aaaaaggcgc
     1021 gcccggacct cctccggcat tccgcacccg ttatcctcga ctgtgatctc ataggtttcc
     1081 gggccggtca cgcgggcacg gaccctgatc caggcatccg gtttatcttt aaaagcctcg
     1141 aacgcgttct tgacaaggtt cagaagcgcg cgataaagcg agcgaccgtc cccgcggacg
     1201 accggaaaat ccttttcagc ggcacaatca agctttacgg agagcgcccc cgcgcgttcc
     1261 gcgaggtcct cgcccaactc ttgtagcagg gccgggacat ccacatcttc gggttcgagc
     1321 tgatagtctt tgctgaggcc ggccacttcc cagataaaac cgtccatatc cttcagagtc
     1381 ttttcgagct tcccgaggcc ggtcgcgatg aacttcgggt tcttttcatt aacgcccatc
     1441 cggatcattt catccgaaaa tttcgcgagc tggagaatgt tcttgatctc atggaccagc
     1501 atcgccgtgg tctggccgat cgcggcaagc cgttccatct tgaggttttc ttcgaaaaga
     1561 aggatgtttt tgagcgcgcc ggaaaactcc gatgccagat taaaaccggt ctccgtctcc
     1621 cgcgacgtga acgcggcggc gcgctccaga caggcaagtc cgacggaggc attgccggcg
     1681 ataaggggaa ggaaaagaag acctttttcc gcggagggtt tcccgtcatc aaaagccgcc
     1741 tgggcagccc ctaggagaac cgcggacgct tctttgggag taaaggtcgc gctttcaagc
     1801 cacagctgtt tatccgggcc ggccaggaac agatagccgc gcgaggaagg gacaagctgc
     1861 aaaagggtc
//
LOCUS       DAOOBX010000193         1868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_68218_length_1868_cov_3.011031, whole genome shotgun sequence.
ACCESSION   DAOOBX010000193 DAOOBX010000000
VERSION     DAOOBX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68218_length_1868_cov_3.011031"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..962
                     /locus_tag="PLY30_03310"
     CDS             186..962
                     /locus_tag="PLY30_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW65167.1"
                     /translation="MKALGGRLEIVMMPPRPCRNLDEILTRHNLDGLMILTWRWIHPS
                     VARLIETQKHPRVLVVNDPLPGLKVNHVYTDAVHGTRLAVDRLVGKGIRGIGMVHGPL
                     AISFRTGDRVRKSPFIDTILKRNAFLKALKARKVPAEKKWIRAAAANSEAEGYRVMKT
                     WVRERELPAAIVCGNDDLAFGVIRALKAAGLRCPGDILVTGFDDNGKAAGFRPPLTTI
                     RQPLQRMGKDAVDILFRQIRKPGRAVAKKYPPKLIVRKSA"
     gene            1082..>1868
                     /locus_tag="PLY30_03315"
     CDS             1082..>1868
                     /locus_tag="PLY30_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HPW65168.1"
                     /translation="MLITVIAAVIGLGYFFYPPRFGWVRASVKYLAGVYCFWAAILFA
                     QPPVQGPVWRTSGLIILFVCAFLLIADMVMVLVSWVSDRLSLLFKRKIKLPAYLNEIW
                     TAYENLAARKVGALIVLRRGQPLAARMRGPGIPFDAEIRSEILIPLFLTTSPVHDGAV
                     IVQKGRITMVKAILPLASLMDGSVNFGTRHRAALGITEQTDAVALVVSEERRQITIAY
                     RGSLVSIPDRGEFERVMNWILKGRNILKLKGVRFVVTTKVLEDF"
BASE COUNT          355 a          574 c          563 g          376 t
ORIGIN      
        1 gacgagtcga gcgactcctg gcaggccgtc tcggtcgaga ttcccggcgc cggggaatga
       61 aggctgtacg aagcgcggat tttcgcggcc gtgcccgttt cgttctcggg attctgacga
      121 ccccccagaa agatatcttt cattccggtt atcatctcgg gctcctttcc ggaatcctgc
      181 cccgggtgaa agcgctcgga gggcgcctcg agatcgtcat gatgccgccc cgcccctgca
      241 ggaacctgga tgagattctg acccgccaca accttgacgg gctcatgatc ctcacgtggc
      301 ggtggatcca tccctcggtc gcgcgactga tcgaaacgca gaagcatccg cgcgtgcttg
      361 tcgtgaacga tcccttgccg gggctcaaag tgaaccatgt ttacacggac gcggttcacg
      421 gcacgcggct cgccgtggac cgtctggtcg ggaaggggat tcgtggtatc ggaatggtcc
      481 acgggccgct cgcgatcagt ttcaggaccg gcgacagggt ccggaagtcc ccgttcatcg
      541 acacgatcct caagcgaaac gcctttctca aagcactgaa agcccggaag gtccccgccg
      601 aaaaaaaatg gatacgcgcc gcggccgcga acagcgaagc ggaaggttat cgtgtcatga
      661 aaacgtgggt ccgcgaacgg gaacttcctg cggcgatcgt ttgcggaaac gatgaccttg
      721 cgtttggtgt gatccgcgcc ctgaaagccg cgggactccg gtgccccggg gacattctcg
      781 tcaccggttt tgacgacaac ggaaaagccg ccggctttcg gcctcctctt accacgatcc
      841 gccagccgct gcaaaggatg gggaaggacg ccgtcgatat tcttttccgg cagatccgaa
      901 agcccggccg ggcggtcgca aaaaaatacc cgccaaaact gatcgtccga aaatccgcct
      961 gagcggtccg aagcgccggg ccccggcttc cggactcacg gcttgtcacg acgatgcagt
     1021 caatccaatc cggattcctg ccgtaatata aggtgcaagg cacctcaaaa aagggaaaat
     1081 catgcttatt acagtcatcg cggccgtgat cggactcggt tattttttct atcctcccag
     1141 gttcggctgg gtcagggcgt ccgtcaaata cctggcgggc gtctattgct tctgggccgc
     1201 catcctcttc gcgcagccgc cggtccaggg accggtctgg cgcacatcgg ggctcatcat
     1261 tcttttcgtc tgcgcgtttc ttctcatcgc ggacatggtg atggtgcttg tgagctgggt
     1321 ctcggaccgt ctgtcgctgc tctttaaaag aaagatcaaa ttgcccgctt acctgaacga
     1381 gatctggacc gcctacgaga acctcgccgc ccgcaaggtt ggcgcgctca tcgtgctccg
     1441 gcgcggtcag ccgctcgcgg cccgcatgcg cggccccggc attcccttcg acgcggagat
     1501 ccgctccgaa atcctgattc cgctcttcct cacgacgtca ccggttcacg atggcgccgt
     1561 tatcgtccag aaaggacgga tcacgatggt caaggcgatt ctgccgctcg cgtcgctgat
     1621 ggacggctcg gtgaatttcg gcacgcgcca ccgcgcggcg ctcggcatca cggaacagac
     1681 ggacgcggtc gcgcttgtgg tttcggaaga acggcgccag attacgatcg cgtatcgcgg
     1741 cagtctcgtg agcatccccg accgcgggga gtttgaacgg gtcatgaact ggattctgaa
     1801 gggccggaac atcctcaagc tgaagggcgt ccggtttgtc gtgacgacca aggtgctgga
     1861 ggatttcg
//
LOCUS       DAOOBX010000194         1866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_68323_length_1866_cov_2.757040, whole genome shotgun sequence.
ACCESSION   DAOOBX010000194 DAOOBX010000000
VERSION     DAOOBX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68323_length_1866_cov_2.757040"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PLY30_03320"
     CDS             <1..1007
                     /locus_tag="PLY30_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPW65169.1"
                     /translation="RPTPLNTFAPGVEVHAQIAEQMFTGDYLRRPDWTEGAEFLYICV
                     LGILLIAFLPSVGAVWCAFLGAAAIGFAVWYSWKLYVDENLLIDPLVPSLSILLIYLV
                     SSFLRFLKTETERGQIRGAFARYLSPELVTQLAKNPKQLKLGGETREMTFLFTDIRGF
                     TTLAEGLNAQELTVFMNSFMTPMTEIILKHKGTIDKYIGDCIMAFWNAPLSDADHARN
                     ACHAALEMREFLSVFNERRKKEAEAQGRPFREIKIGIGINTGLAAVGNMGSEYRFDYT
                     VLGDEVNLASRLEGLTKQYETTIIIGPNTANRIPELKPQELGTIQIRGMTVPKQIFSL
                     "
     gene            complement(1058..>1866)
                     /locus_tag="PLY30_03325"
     CDS             complement(1058..>1866)
                     /locus_tag="PLY30_03325"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPW65170.1"
                     /translation="VVHGAAESVSPDGTVKELKAGDPVFLDEQVVTKDDGRLQILLMD
                     ETVFTLGPSSAIRLDDFAYDPVTQDGILNTDILRGVFRFISGKIAHKKPENMSVGLPA
                     GSIGIRGTVVAGRSEGFRSLVVLLGPAGDDVTPPGRIFVSNDIGGAQAGVEISQIGFG
                     TVIEGPGTTPAPVFRVPEADLNGLMDELNLSSNGYAATVPNAATFGASAKDIDDMTRT
                     LSLMSQMEEYGHRATQGGMMSLPQPVMPGAPAAGRGSDDGSDSGNSSSGG"
BASE COUNT          408 a          595 c          494 g          369 t
ORIGIN      
        1 tccgccccac cccgctcaac accttcgcgc ccggcgtcga ggtccacgcc cagatcgcgg
       61 agcagatgtt cacgggcgat tacctgcgcc gtcccgactg gaccgaaggc gcggaattcc
      121 tttatatctg cgtgctcgga atcctcctga tcgcgtttct cccgagcgtc ggcgcggtat
      181 ggtgtgcttt cctcggcgcc gcggcgatcg gcttcgcggt atggtattcc tggaagctgt
      241 acgtggacga aaacctttta atcgaccccc tcgtcccttc gctttcgatc ctcctgattt
      301 acctcgtctc ctcgttcctc aggttcctca agacagagac cgagcggggg cagatccgcg
      361 gtgcgttcgc ccgttatctt tcgcccgagc tcgtgacgca gcttgcgaaa aacccgaaac
      421 agctgaagct gggcggcgaa acgcgtgaaa tgacctttct ttttaccgat atccgcggat
      481 tcaccacgct tgcggaaggg ctaaacgcgc aggagcttac ggtcttcatg aactccttca
      541 tgacccccat gacggagatc atcctcaagc acaagggcac gattgacaaa tacatcgggg
      601 actgcatcat ggcgttctgg aacgcacccc tcagcgacgc ggatcacgcg aggaacgcct
      661 gtcacgcggc gcttgaaatg cgggaattcc tctcggtatt caatgagagg cggaaaaagg
      721 aagcggaggc acagggcaga cccttccgcg aaatcaagat cgggatcggg atcaatacgg
      781 gcctcgcggc ggtcggtaac atgggttcgg aataccggtt cgactacacg gtcctcggag
      841 atgaggtgaa cctcgcatcg cggctggaag gtctcacgaa acaatatgag acgaccatca
      901 ttatcggccc caacaccgcg aaccgcatac ccgaactcaa acctcaggaa ctcgggacca
      961 tacaaatccg cggcatgacc gttcccaaac agatcttcag cttataaaaa acggggtcag
     1021 tcaccgtttt agagaaatgg tgactgaccc cgtttttcta gccgccggac gaggaatttc
     1081 cgctgtcgct gccatcatca ctgccgcgac cggcggcggg cgcccccggc atcacgggct
     1141 gcggcaatga catcatccct ccctgcgtcg cgcggtggcc gtattcctcc atttgcgaca
     1201 tgagcgacaa cgtccgggtc atatcatcaa tatctttagc ggacgcccca aaggtcgcag
     1261 cgtttggaac cgtcgcggca taaccgttcg aggaaagatt cagttcgtcc atcagcccgt
     1321 tcagatccgc ttcaggaacc cggaaaacgg gcgccggcgt ggtaccgggg ccttcaataa
     1381 cggtcccaaa accaatctgc gaaatctcca cgccggcctg ggccccgcct atatcattgg
     1441 acacaaaaat gcggcccggc ggcgtcacgt catcccccgc cggccccaaa agcacaacga
     1501 gagagcggaa cccttccgat ctgcccgcca ccacggtccc gcggataccg atacttccgg
     1561 cgggaaggcc cacggacatg ttttccggtt tcttgtgtgc gatcttcccc gaaataaacc
     1621 ggaacacgcc ccggaggata tccgtgttca ggattccgtc ctgggtcacc ggatcataag
     1681 cgaagtcatc gagcctgatc gcactcgacg gaccgagtgt aaaaacagtt tcatccatca
     1741 gcaaaatctg gagtcgcccg tcatcttttg tcaccacctg ttcatcgaga aatacgggat
     1801 ctccggcttt cagctctttc acggtgccgt ccggtgacac agactccgcc gcaccgtgaa
     1861 ccacac
//
LOCUS       DAOOBX010000195         1866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_68333_length_1866_cov_2.427388, whole genome shotgun sequence.
ACCESSION   DAOOBX010000195 DAOOBX010000000
VERSION     DAOOBX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68333_length_1866_cov_2.427388"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..>1866
                     /locus_tag="PLY30_03330"
     CDS             113..>1866
                     /locus_tag="PLY30_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPW65171.1"
                     /translation="MKIISLMKQVPLPTEMRMGADGLMDRTKAKSMINADCAFGLEQG
                     LQLKKHVPDAELIVIAMGPTSFEQSLKKALAMGYDRAILLSDRKLGGSDTFATGYAIS
                     SLIKKLDLTGPYVIFSGRQTTDGDTAHVPSQTAENLGIPQATFVEKVEFQGDHVKVRR
                     IIEGGNQTLRLPVPCLISIAPTATPARRPSLEGAIRARSAKVEVWTLEQTGADVSVVG
                     LTGSPTLVAKVVDIQKNRPPVNMISGRTNPEIADGLAAEIEKARDRIAGGHADAAPAV
                     GPKEAAEAKVDPGFPRVDFRNGARGVMTWVEMYGQTPARSSLEILSQARKLADHLGTK
                     VRSVMVGHNIKVAAQEVIEYGADEVVVVDDPRLREYTILPTTAALAQIIEKERPEIAL
                     FGATTSGRELAPRLASRVRAGVTADCTSLEVGEYVHRVKKSVFYPVLHSIRPTYGESK
                     LATIIGFWCPQMATARAGTFEILPRDSKRKGTVTEFKPVFGDSDFAVEVLETKRESGG
                     GDNLFVADIVISGGRPAGDQDDFKLIRELVTALREKGINADWGASRHAVDNGYAPYAR
                     QIGQTGKTIRPKIYLAVA"
BASE COUNT          379 a          543 c          605 g          339 t
ORIGIN      
        1 gaccggagta cgacgatctg tagacgggta tggagcacgg tgcgcggcga aaaactcttt
       61 gttctccgta ctccctgcct cgtacgccgt attcaataag gaatctatgg gcttgaaaat
      121 tatcagttta atgaaacagg taccgctgcc gaccgagatg cggatgggcg cggacggcct
      181 catggaccgg acgaaggcga agtccatgat caacgccgac tgcgcgttcg ggctcgagca
      241 gggactccag ttgaagaaac acgttccgga tgcggagttg atcgtgatcg cgatgggacc
      301 gacgagcttc gagcagtccc tcaagaaggc gctcgcgatg ggctatgacc gggcgattct
      361 tctttccgac cgtaaactcg gcggctcgga cacgttcgcg acgggatacg cgatctcctc
      421 cctgatcaag aaactcgatc tcacaggtcc gtacgtcatt ttttcgggac gacagaccac
      481 ggacggggat accgcgcatg ttccctcgca gaccgccgag aacctcggca tcccgcaggc
      541 cacctttgtc gagaaggtcg agttccaggg ggatcacgtc aaggtccgcc gcattatcga
      601 aggcgggaac cagaccctcc gccttcctgt tccgtgtctt atttcgatcg cgccgaccgc
      661 gacgccggcc cgccgtcctt cactcgaagg agcgatccgc gcgcgaagcg caaaagtgga
      721 ggtctggacg ctcgagcaga ccggcgcgga tgtcagcgtg gtgggcctga ccggttctcc
      781 gacgcttgtc gcgaaggtcg tggatatcca gaagaaccgg ccgcccgtca acatgatctc
      841 ggggcgcacg aacccggaga tcgcggatgg tcttgccgct gagatcgaga aggccaggga
      901 ccggatcgcg ggtgggcacg cggatgccgc tccggccgtc ggtcccaaag aagccgcgga
      961 ggcaaaggtg gatcccggat tcccgcgcgt cgattttcgt aacggcgctc gcggtgtgat
     1021 gacctgggtc gagatgtacg ggcagacacc cgcccgttct tcgctcgaga tcctgagcca
     1081 ggcccgcaag ctcgctgacc atctcggcac gaaggtgcgt tccgtgatgg tcgggcacaa
     1141 tatcaaggtg gctgcgcagg aagtgatcga atacggcgcc gatgaagtgg tcgtcgttga
     1201 tgatccgagg ctcagggagt acacgatcct cccgacgacg gcggctctcg cgcagatcat
     1261 cgaaaaagag aggcccgaga tcgcgctttt cggcgcgacg acttccggcc gtgaactcgc
     1321 gccgcgtctc gcgagccgcg tgcgggcagg cgtgacggcc gactgcacga gcctcgaggt
     1381 gggcgaatac gtccaccgcg tgaaaaaatc cgttttttat cccgtccttc attcgatccg
     1441 tccaacctac ggcgagagca agcttgcgac gatcatcggt ttctggtgcc cgcagatggc
     1501 gaccgctcgg gccgggacgt tcgagatcct gccccgggat tcaaaacgca aggggaccgt
     1561 taccgagttc aaacccgtgt tcggggacag tgattttgcg gttgaggtcc tcgagacaaa
     1621 acgcgagtcg ggaggcgggg acaacctttt cgtggcggat attgtgatct cgggaggacg
     1681 tccggcgggg gaccaggatg atttcaagct gataagggaa cttgtcacgg ccctgcggga
     1741 aaaagggatt aacgccgact ggggcgcgag ccgccacgcg gtcgacaatg gttacgcgcc
     1801 gtacgcccgc cagatcgggc agacggggaa aacgatccgg ccgaagattt atctcgcggt
     1861 cgcaat
//
LOCUS       DAOOBX010000196         1859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_68680_length_1859_cov_3.201774, whole genome shotgun sequence.
ACCESSION   DAOOBX010000196 DAOOBX010000000
VERSION     DAOOBX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68680_length_1859_cov_3.201774"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /gene="mutL"
                     /locus_tag="PLY30_03335"
     CDS             complement(<1..755)
                     /gene="mutL"
                     /locus_tag="PLY30_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPW65172.1"
                     /translation="MGTIHVLPEHVANKIAAGEVVERPASIVKELVENSLDAGADRIE
                     IAVRHGGKSLIRVADNGPGMSGEDVQLAVQRHATSKIASAEDLGGILSFGFRGEALPS
                     IAAVSRFTIQTRPARSTSGTELVIEGGSLVRARECPTGQGTVIEVRDLFFNTPARRKF
                     LKQDSTEFGHILDAVICLALANLDVHFTLCSQDKEILDLLPAGDVAARARAVFGDEWA
                     DQVLAVSGEIPYAKLTGLIGKPSLASGNRTSQF"
     gene            complement(758..>1859)
                     /locus_tag="PLY30_03340"
     CDS             complement(758..>1859)
                     /locus_tag="PLY30_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPW65173.1"
                     /translation="VVPELKAWDEKISGAQDKIKALEYQIFLEVRQQTLADLAALQRM
                     ARDVGTLDVLMSFARIAIEKKWCMPEVNDGHAITIDGGRHPVVESMLPSGKFVENDLS
                     LDARGDQLIVLTGPNMAGKSTYIRQAAQIVLLAQIGSYVPAQKAIVGLVDRIFTRIGA
                     SDDLARGESTFMVEMLETAHILQEATERSLLILDEVGRGTSTFDGVSIAWAICEYLAQ
                     GRRPRTLFATHYHELTQLEGHFPGTRNYKMAVKETPEGILFLRKVVPGASERSYGIHV
                     AELAGLPKQVTQRAKEILAILENENTEATEIIETGKKRGTKKVADSGPTLFDVAAPRP
                     GEVEETLKKIDTDRLTPIEALQKLAELKKKVK"
BASE COUNT          356 a          596 c          497 g          410 t
ORIGIN      
        1 ataaattgcg acgtccggtt tcccgatgca agcgaaggtt tcccgatcag tcccgtcagc
       61 ttggcgtatg ggatctctcc cgacacggcg agcacttgat ccgcccattc atcgccaaaa
      121 acagcgcgcg cccgggcggc aacatctccg gccggaagaa ggtcgagaat ctccttgtcc
      181 tgcgaacaca gcgtgaaatg gacgtccaaa ttcgcgagcg cgagacagat cacggcgtcc
      241 aggatatgac caaattccgt ggaatcctgt ttcaggaact tccgccgcgc cggtgtatta
      301 aagaacagat cgcgcacttc aatcacggtt ccctgtcccg tcgggcattc ccgggccctg
      361 acaaggcttc ccccctcgat cacaagctcg gtacccgagg tcgaccgcgc aggtcttgtc
      421 tggatcgtga atcgcgatac cgcggcgatg ctcggaagcg cctcgccgcg aaaaccaaaa
      481 ctcaggatcc cccccaagtc ttcggccgac gcgatcttgc tggtggcatg tcgctggaca
      541 gcgagctgaa catcctcccc gctcatgccc ggcccgttat ccgcgacccg tatcaggctc
      601 ttgccgccgt gacggacggc gatctcgatc cggtccgcgc cggcatcgag cgaattctcg
      661 acaagttcct tgacgatgga ggccggccgc tcaaccactt cccccgccgc gatcttgttg
      721 gcaacatgtt ccggcaagac gtgtattgtg cccatgatta tttgaccttt tttttcaatt
      781 ccgcaagttt ctgaagcgcc tcgatcggcg tgagccggtc cgtatcgatc tttttcagag
      841 tctcttcgac ttcgccgggc cggggggccg cgacatcaaa aagcgtgggc ccgctgtctg
      901 cgactttttt tgtgccgcgc tttttccccg tctcgatgat ctcggtcgct tcggtattct
      961 cattttcaag gatcgcgagt atctccttcg cgcgttgcgt gacctgtttc gggagcccgg
     1021 cgagttcggc gacatggata ccgtagctgc gctccgaggc gccggggacg accttcctca
     1081 ggaagagaat tccctccggg gtttccttga ccgccatctt gtagttccgg gtaccgggga
     1141 aatgcccctc aagctgggta agctcgtgat aatgagtcgc gaaaagagtc cgtggtcggc
     1201 gcccctgcgc gagatattcg caaatcgccc aggcgatgct tacaccatca aaagtcgaag
     1261 tcccgcgtcc cacctcgtcc aggatcaaaa ggcttcgttc cgtcgcctcc tgaaggatat
     1321 gggcggtctc gagcatttcc accataaatg tcgattcgcc gcgcgccaga tcatcggacg
     1381 cgccgatgcg cgtaaagatc cggtccacca gaccgacgat cgccttttgc gcgggcacat
     1441 acgaaccgat ctgggcaaga agaacgatct gagccgcctg acggatgtac gtcgatttgc
     1501 cggccatgtt cggtcccgtc agcacgatca gctggtctcc cctggcatcg agcgaaagat
     1561 cattctcgac gaattttccg ctcggcaaca tgctttccac aacgggatgc ctccccccgt
     1621 cgatcgtgat agcatgcccg tcattcactt cgggcatgca ccatttcttc tcgatcgcaa
     1681 ttctggcgaa cgacatcaag acatccagtg tcccgacatc cctggccatc ctctgcagcg
     1741 cggcaaggtc cgcgagggtc tgctgtcgaa cttccaggaa gatctgatat tcgagcgcct
     1801 tgatcttgtc ctgcgctccg gagatcttct cgtcccatgc cttcagctcc ggcacgaca
//
LOCUS       DAOOBX010000197         1845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_69452_length_1845_cov_3.792737, whole genome shotgun sequence.
ACCESSION   DAOOBX010000197 DAOOBX010000000
VERSION     DAOOBX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69452_length_1845_cov_3.792737"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PLY30_03345"
     CDS             complement(<1..972)
                     /locus_tag="PLY30_03345"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPW65174.1"
                     /translation="MHKKNLRIMAMILAGTLAVAGVPGPVHAEDPNRNYPAYADPGSP
                     YGSPPEPYAGVPASALAAKETPKMQVERTDAGKFFLDFKSASLINVLNVIASLSGINF
                     VAGKEVAERHVNMTLADVTLEDALQAIAHGSNVSYDFIPGRNIYLFRASADAPEQPPL
                     LTRVFKLYYIRVSKPKEIEVSSSGSSGSSGSSGSIGGGLTTLTQNNDNEDDTDLEGAA
                     VYAAVEKILSDRGKVSVDDRTNSLIVTDSEDRLKMISAALQQLDRPVDQVLINVLLVE
                     TFEDLSRAVGIRWGDQSGNLGTITGGVSSTDWPFRFNGSTQETQSLGD"
     gene            complement(1023..1499)
                     /locus_tag="PLY30_03350"
     CDS             complement(1023..1499)
                     /locus_tag="PLY30_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65175.1"
                     /translation="MTKRIVMVLMLLAFVSGAVPVVARGEEPLKDRLSEGTSISTAVE
                     NAQQEVRDPFASSIPAEKPAAPVVAAPVVSNIKLQGIGVGSEDAYAVMNGDVFFEGEE
                     KNGIKLVSVRKREVDIETGGLPQTLSMLSESELKKLQQRRTRKPVSEQREQDTASF"
     gene            complement(1496..>1845)
                     /locus_tag="PLY30_03355"
     CDS             complement(1496..>1845)
                     /locus_tag="PLY30_03355"
                     /inference="COORDINATES: protein motif:HMM:NF022201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HPW65176.1"
                     /translation="VARREALLANMDDRPILIAKLSDLASQQKVELQGLIPRTENADN
                     YVRLRAEADMRCTYFALLNYLKVLEQMRPPVFIQDISMTQTRSGGRGSEKEVPLLQVR
                     MIMDTYLRQKAGK"
BASE COUNT          341 a          562 c          523 g          419 t
ORIGIN      
        1 atcccccaga ctttgcgttt cctgggtact gccgttaaag cggaaaggcc agtcggtcga
       61 tgacacgccg cccgtgatcg tgccgaggtt gccgctctga tcgccccacc ggataccgac
      121 cgcgcggctg aggtcctcga acgtttcgac gagcaggaca ttgatgagaa cctgatcgac
      181 gggacggtcc agctgctgga gcgcggcgga gatcatcttg agccggtcct ccgaatccgt
      241 cacaatgagg ctgttcgtcc ggtcatccac ggagactttt ccgcggtccg agagtatctt
      301 ttcgacggcc gcatacaccg ccgccccttc caggtcggtg tcatcttcat tatcgttatt
      361 ctgggtcagg gtcgtcagcc cgccgccgat gctgccggaa ctgcccgaac ttccggaact
      421 gccgctgctc gatacttcga tctctttcgg cttgctgacg cggatgtaat agagcttgaa
      481 cacgcgcgtc aaaagcggtg gctgttcggg ggcgtccgcc gaagcgcgaa aaagatagat
      541 gttccggccg gggatgaaat catagctgac attgctgccg tgggcgatgg cctgaagcgc
      601 atcctcgagg gtcacatcgg caagcgtcat gttgacatgg cgttcggcga cctctttgcc
      661 ggccacgaag ttgattccgc tcaatgaggc gatcacattc agcacgttga tcagggacgc
      721 ggatttgaag tcgagaaaga actttccggc atctgtccgc tcgacctgca ttttcggggt
      781 ttccttcgcc gcgagcgcgc ttgccggcac tccggcgtac ggttcgggcg gactgccgta
      841 cggtgacccc gggtcggcat aagccggata gttcctgttc gggtcctcgg catggaccgg
      901 cccgggcacc ccggcgaccg ccagagtccc cgccaaaatc attgccatga tcctgagatt
      961 ttttttatgc atgatccgtt ctcccctttc ttgggaaaca agctgtcaat atcccgcgag
     1021 cgtcaaaatg aagccgtatc ctgttcccgc tgttccgaaa ccggtttccg ggtcctccgt
     1081 tgctgaagtt ttttcagctc gctttcggaa agcatggaca atgtctgcgg gaggcctccg
     1141 gtctcgatat ccacttcccg tttccggacc gagacaagct tgataccgtt cttttcctcg
     1201 ccctcgaaga agacatcgcc gttcatcacc gcgtaggcgt cctcggaacc gacgccaatc
     1261 ccctgcagtt tgatgttcga gacgacaggc gccgcaacga ccggtgccgc gggtttttca
     1321 gccgggatcg aggaagcgaa cgggtcccgc acctcctgct gggcgttctc gaccgcggtg
     1381 gaaatggaag tcccttccga cagtctgtct tttaagggct cctcgccgcg cgcgacgaca
     1441 ggcaccgcgc ctgagacgaa tgccaggagc atcaggacca ttacgattcg ctttgtcatt
     1501 ttcccgcctt ttgtctcaga taagtgtcca ttatcatccg gacctgcagc agagggactt
     1561 ccttttcgga tccccgaccg ccgcttcgcg tctgtgtcat gctgatatcc tgaatgaata
     1621 cgggaggccg catctgttcc aggaccttca gataattcag cagcgcaaaa taagtgcatc
     1681 gcatgtccgc ctcggcccga agccggacat agttgtcggc gttttccgtc cggggtatca
     1741 gcccctggag ctcaactttt tgctgacttg ccagatcgga aagcttggcg ataaggatcg
     1801 ggcggtcatc catgttcgcc agcaacgcct cgcggcgggc aacct
//
LOCUS       DAOOBX010000198         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_69577_length_1843_cov_2.612416, whole genome shotgun sequence.
ACCESSION   DAOOBX010000198 DAOOBX010000000
VERSION     DAOOBX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69577_length_1843_cov_2.612416"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..840)
                     /locus_tag="PLY30_03360"
     CDS             complement(121..840)
                     /locus_tag="PLY30_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPW65177.1"
                     /translation="MKYAVLADIHGNLEALEAIKAYLEARNLTTILCLGDSIGYGANP
                     NECFEWVLAHASLHVLGNHEAAVFHEKTRWSFNDWSRVAIDWTAERLRPELVKKIESL
                     PYLRVRGPLTVAHGTIHRPGDFEYLMDDVSADKSFRALGTDVGIVGHSHIPCWFSEAK
                     PGAGGYFREGVLRLEKGDRYLLNPGSVGQPRDRDPRLSFGIFDPGELTFEIVRLPYDN
                     KKAARKIRAEGLSRVFADRLL"
     gene            complement(931..1203)
                     /locus_tag="PLY30_03365"
     CDS             complement(931..1203)
                     /locus_tag="PLY30_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPW65178.1"
                     /translation="MKRMQVFFSGTVQGVGFRFTTERVARRFAVTGFVRNLDDGRVEV
                     VAEGEEPVLVEFLTAIRESGMKYYIENVEAHWSDAAGTYKRFGIVM"
     gene            complement(1207..>1843)
                     /locus_tag="PLY30_03370"
     CDS             complement(1207..>1843)
                     /locus_tag="PLY30_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPW65179.1"
                     /translation="NNIRSLHNVGSIFRTADGAGVEKIYLCGITGHPPDTKITKTALG
                     AEKEVPWTYEREAAIVLGKLKAEGYEIVLLEQLEQSVPYEAFTPSERVCLVVGNEIEG
                     VSDGLLPLCDRTIEIGMAGLKNSLNVSVAFGIAAFHIRNALRNGYPVRRRANPSESLR
                     RTSHAARNDLTVKKAGEICETAARIKKTGTRDAGHDDSHDRFSCRAPDPIL"
BASE COUNT          347 a          584 c          516 g          396 t
ORIGIN      
        1 gcttcgatct gctttttagc ttcccgcaat ttcataatct ttcctgtccg gagtacgatg
       61 cacggggtcc ggagtaagga gcacgatctt tgacctccgc actccggtcc ccgcgcctgt
      121 ttaaagcagg cggtccgcga acacccgcga cagtccttcg gcgcgaattt ttcgggcggc
      181 tttcttatta tcataaggca gccggacgat ttcaaatgtt aattccccgg gatcgaaaat
      241 cccgaacgaa agcctcgggt cgcggtcgcg gggctggccg acgctgcccg gattgaggag
      301 gtagcggtcc cctttttcaa gacgcaggac accctccctg aaataaccgc ccgccccggg
      361 cttcgcttcc gagaaccagc acgggatgtg actgtgcccg acgatcccca cgtccgtccc
      421 gagcgcccgg aaactcttgt cggccgagac gtcgtccatg agatattcga aatccccagg
      481 ccggtgtatg gtcccgtgcg cgaccgtaag cgggccccgg acccggaggt acggaaggct
      541 ctcgatcttt ttaacgagtt ccgggcgaag cctctccgcg gtccagtcaa tcgcgacgcg
      601 ggaccagtcg ttgaagctcc accgtgtttt ttcgtgaaag acggccgcct cgtggttccc
      661 gagcacgtga agcgaggcat gcgcgagcac ccactcgaaa cattcgttcg ggttggcgcc
      721 gtaaccgatc gaatccccaa ggcagaggat tgtcgtgaga ttgcgcgcct cgagatacgc
      781 ttttatggcc tcaagcgcct ccaggttccc gtggatatcg gcaagaacag cgtatttcat
      841 ccgtactccc tatgagcgcc gggtgtcgcg ccaaacgaaa cccgaaactt ttccaccccg
      901 ggttcttcgc ccctgcgccg cgtgtgtttt ttacataacg attccgaacc gtttgtaggt
      961 cccggcggcg tccgaccagt gagcctcgac gttttcgatg taatacttca tacccgactc
     1021 gcggatcgcc gtcaggaatt cgacaaggac cggctcctcg ccctccgcga cgacctcgac
     1081 gcgcccgtcg tcaaggttcc gcacgaatcc cgtcaccgcg aaccgccgcg cgacccgctc
     1141 cgtcgtgaag cggaaaccca cgccctgcac cgtcccgctg aagaatacct gcattcgttt
     1201 catggttcag aggatgggat ccggagcgcg gcacgagaac ctgtcatggg aatcatcatg
     1261 ccccgcatcc cgtgtcccgg tttttttaat tcgggcggcg gtttcacata tctcgccggc
     1321 ttttttcacc gtcaggtcat tgcgagccgc atgcgaagtt cgcctcaagg attcggacgg
     1381 attcgccctc cggcggaccg ggtaaccgtt tctgagggca ttccggatat gaaaggccgc
     1441 gatgccgaac gcgaccgaaa cattgagcga gttcttgaga cccgccatgc cgatctctat
     1501 cgtccggtcg cagagcggga gaaggccgtc ggagacgccc tcgatctcgt taccgaccac
     1561 gaggcagacg cgctccgaag gcgtgaaggc ctcatacgga acactctgtt ccagttgttc
     1621 gagaagcacg atctcatagc cctccgcttt cagttttccg agaacaatcg ccgcttcccg
     1681 ctcgtacgtc cacggcactt ccttttcggc cccgagcgcg gttttcgtga ttttcgtgtc
     1741 gggaggatgt cccgtgatgc cgcagaggta gattttctcg actccggcgc cgtccgcggt
     1801 gcggaagatg gaaccgacgt tatgaaggct ccggatatta ttg
//
LOCUS       DAOOBX010000199         1835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_70000_length_1835_cov_2.457865, whole genome shotgun sequence.
ACCESSION   DAOOBX010000199 DAOOBX010000000
VERSION     DAOOBX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70000_length_1835_cov_2.457865"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..523)
                     /locus_tag="PLY30_03375"
     CDS             complement(107..523)
                     /locus_tag="PLY30_03375"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HPW65180.1"
                     /translation="MILDEIKKIDSSPRKVREFGWVMAVFFGLLGALLLWRGKPSAGI
                     FLILGIAFAILTLIARPILKPLQKVWMLIAVVMGWVMSRVILGILFYLAITPVALILR
                     LRGKDLLDIKLDPAAASYWKHRANEPKSRTHCERQF"
     gene            complement(607..>1835)
                     /locus_tag="PLY30_03380"
     CDS             complement(607..>1835)
                     /locus_tag="PLY30_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HPW65181.1"
                     /translation="KVMGLAPYGKPVYKDLILKELIDLKEDGSFKLNMKYFDYVAGLR
                     MTNTKFHKLFGGPPRKPESRVTQREMDLARSIQEVTEEVMLRVARHVHKTTGMQNLCL
                     AGGVALNCVGNGRILREGPFEKIWIQPASGDAGGALGAALYAWYHYLQNPRTVDGKKD
                     LMTGSCLGPEYSRDEIRDFLTKEEIPFRELPDEKIAGTVADLIAQEKVVGWFQGRMEF
                     GPRALGGRSIIGDSRSPKMQEQMNLKIKFRESFRPFAPSVTAEKVSDYFELDCESPYM
                     LLVASVQKKLRREMTAEEQKLEGIDLLNVERSSIPAVTHVDYSARVQTITPEQNPRFY
                     QLLKAFDERHGCPVMINTSFNVRGEPIVCTPGDAFRCFMRTHMDYLVLGNFVLDKTEQ
                     KAPEKDSAWMKEFELD"
BASE COUNT          382 a          523 c          501 g          429 t
ORIGIN      
        1 aggaaatccc aaaactcttt aagaatggat atctttccca tgaatatctc ctgttaaaaa
       61 tgtcggcgcc aggcattcta gcaaaagctg aggaacgcgt catggattaa aactgacgtt
      121 cgcagtgagt gcgggacttt ggctcattcg cccgatgctt ccagtagctc gcggcggccg
      181 gatcgagttt tatgtccaga aggtctttcc cccgcaagcg caggatcagg gcgaccggcg
      241 tgatcgcgag gtagaaaagg atgccgagga tcacgcgcga catcacccag cccatcacga
      301 ccgcgatcaa catccagact ttctggagcg gtttcagaat cggccgcgcg atgagggtca
      361 atatggcgaa tgcgatgccg aggatcagga agattcccgc ggacggtttc ccccgccaga
      421 ggagcaaggc gccgagcagg ccgaagaaca cggccattac ccagccgaat tccctgacct
      481 tcctggggct cgaatcgatc tttttgattt catccagtat cataggcgat tatcttctgc
      541 tccagtcgtt aaacccttcc acccccggga gatcgacccg ggtcgatctc ccgggggtgg
      601 cgtgggtcaa tccagctcaa attccttcat ccacgcggaa tctttttcgg gtgctttctg
      661 ttcggtctta tccaggacga aattgccgag gacaaggtaa tccatatgcg tccgcataaa
      721 acagcggaac gcgtccccgg gcgtgcaaac gatcggctcc cctctcacgt tgaacgaggt
      781 attgatcatg accggacatc cgtgcctttc atcaaacgcc ttgagcaatt gataaaaccg
      841 cgggttttgc tccggggtga tcgtctggac gcgcgccgaa taatccacgt gcgtcacggc
      901 cgggatcgag gatcgctcga cgttcaacag atcgatcccc tcgagttttt gttcttccgc
      961 cgtcatttcc ctgcggagtt tcttttgaac ggacgcgaca agaagcatgt aggggctctc
     1021 gcagtccagt tcgaaatagt ccgacacttt ttcggccgtc acggaaggcg cgaacggccg
     1081 gaagctttcg cggaatttga ttttcaggtt catctgctcc tgcattttgg gagaacggga
     1141 atcgccgatg atcgaacgac ctccgagcgc ccgcggtccg aattccatgc ggccctggaa
     1201 ccatccgacg actttttcct gcgcgatcag atcggccacg gttccggcaa tcttctcgtc
     1261 cgggagctcc ctgaacggga tttcctcttt cgtcaggaag tcacgaattt cgtccctgga
     1321 atattcggga ccgagacagg agcctgtcat gaggtctttt ttgccgtcca cggttctcgg
     1381 attttgaaga taatgatacc aggcgtaaag cgcggcgccg agagcgcccc ccgcgtcgcc
     1441 cgaggcgggc tggatccaga ttttttcaaa aggcccttcg cggaggatgc gtccgttccc
     1501 gacgcagttg agcgcgacgc ctcccgcaag gcagaggttt tgcattcccg tggtcttgtg
     1561 gacatgccgc gcgacgcgga gcataacctc ttcggtcacc tcctggatgg aacgcgcaag
     1621 gtccatttca cgctgagtga ccctggattc gggctttctc ggaggtccgc cgaagagctt
     1681 gtggaatttc gtgtttgtca tccggagccc tgcgacataa tcgaaatact tcatattcag
     1741 tttgaacgag ccgtcctctt tcagatcgat cagttctttc aggatcaggt ccttgtagac
     1801 cggttttccg tacggcgcga gtcccataac cttgt
//
LOCUS       DAOOBX010000200         1828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_70337_length_1828_cov_3.469825, whole genome shotgun sequence.
ACCESSION   DAOOBX010000200 DAOOBX010000000
VERSION     DAOOBX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70337_length_1828_cov_3.469825"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PLY30_03385"
     CDS             <1..726
                     /locus_tag="PLY30_03385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPW65182.1"
                     /translation="GSGSEATLNAVIINPGKKVKLSIRHPAFLAAGVILDPELLKTVP
                     ESVMAHSGMDALVQAYESYTSKNATLFTETLALRAIEMIGASLIPAAKPAPTDIQPTR
                     LDIGERDKALHDMLFASFLAGLAFSHSRLGVIHGIAHSLGVLYGVPHGLVCAACFIPS
                     IKMNREAMGEKYARMSRALGGDLTEKITTLQTALGITSPFKGKPVIEKEKIIAETLRS
                     GSTAANPKPVEREDVEFLLGELF"
     gene            776..1663
                     /locus_tag="PLY30_03390"
     CDS             776..1663
                     /locus_tag="PLY30_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW65183.1"
                     /translation="MIHFLEYLAASALIALAKALPVRTAAWIACRFADLSYWILPARR
                     RVALGNVGKAFGDSLAASEKRSIVRSAFRSAALSMMELFIVEKTAATARQHFKLLNNE
                     RLEKAFAAGKGVILVIAHLGSWEYLSFLPFLTGQPWSVIVKDIKNPHLDRLITKKRKV
                     MTVNPVPKNNSIKTVLRELKQGRGAAILIDQWAGDDGIWTDFFGTPTSTTSIPARLAR
                     AMGSALVPACCLRTSPGEYEIHIGEPVSWDRDARDWESTVTKQLNAILEQKIREHPGQ
                     WLWGHRRWKPKPANLRETA"
BASE COUNT          393 a          584 c          505 g          346 t
ORIGIN      
        1 ggcagcggtt ccgaggccac gttgaatgcg gtcatcatca atcccgggaa aaaagtaaaa
       61 ctctcgatcc gccaccccgc cttcctcgcc gccggggtga tcctggaccc cgaactcctg
      121 aagacggtcc cggagagcgt catggcccac tcggggatgg acgcgcttgt ccaggcctat
      181 gaatcctaca cctcgaagaa cgcgacgctt ttcaccgaaa cgctggctct tcgagcgatc
      241 gagatgatcg gtgcgagcct cattcccgcc gccaagcccg ccccaaccga tatccaacct
      301 acgaggttgg atatcggtga acgggacaag gcgctgcacg atatgctttt cgcgagtttc
      361 ctcgcggggc tcgcattctc gcattcccgg ctcggcgtga ttcacgggat cgcacactct
      421 ctcggcgttc tctacggcgt gccgcacggc ctcgtctgcg cggcctgctt cattccgtcg
      481 atcaagatga atcgtgaggc catgggagaa aaatacgccc ggatgagccg cgccctgggt
      541 ggcgacctca ctgaaaaaat cacgacgctt cagaccgcgc tcggcatcac gtctcccttc
      601 aaggggaaac ccgtgatcga aaaggaaaag atcatcgcgg agaccctcag gtccggctcc
      661 actgccgcga atccaaaacc ggtcgagcgc gaggacgtcg aattcctcct gggtgagctc
      721 ttctagcaaa ggggactgtc cccttttttt atttcatgct accatcctcc ccgtcatgat
      781 acatttcctg gaatacctcg cagcctcggc gctcatcgca ctcgcgaaag ccctacccgt
      841 ccggaccgcg gcgtggatcg cctgccgttt cgcggacctg tcatactgga tcctcccggc
      901 ccgccgccgg gtcgccctcg ggaacgtcgg gaaggctttc ggggactccc ttgccgcgag
      961 cgaaaaacga tcgatcgtcc gctccgcctt tcgcagtgcc gcgctttcca tgatggaact
     1021 tttcatcgtc gaaaagaccg ccgcgaccgc ccggcagcac ttcaagctcc tgaacaacga
     1081 acggctcgag aaagccttcg ccgccggaaa aggcgtgatc cttgtgatcg cgcacctcgg
     1141 ttcatgggag tacctctcgt tccttccttt cctgaccgga cagccctggt ccgtgatcgt
     1201 caaggacatc aagaacccgc acctcgaccg gctcatcacg aaaaagcgga aggtcatgac
     1261 cgtgaacccg gtccccaaga acaattccat caaaaccgtt ctgcgggaac tgaagcaagg
     1321 gcgcggcgcc gcgatcctga tcgaccagtg ggccggcgac gacggaatct ggacggattt
     1381 tttcggaaca ccgacctcga ccacctctat tcccgcgaga ctcgcccggg ccatgggttc
     1441 ggccctcgtg cctgcctgct gcctgaggac gtctccgggt gaatacgaga tccatatcgg
     1501 ggaaccggta tcgtgggacc gggacgcgcg cgattgggaa agcaccgtga caaaacaact
     1561 gaacgccatc ctggaacaaa agattcgcga acatcccgga caatggcttt ggggacaccg
     1621 gcgatggaaa ccaaaaccgg caaatctccg ggaaacggcc tgaaaaccgc tcaaaacgga
     1681 acggtcaaca caagcaccgg cctgttcgtt gtgtcctgat cgtgaggcat ggggccggat
     1741 ttcaaaatga acattgagct aacaatcgaa aaaatcgcgt tcggcggcga cggtctcggt
     1801 ttcgttgacg ggaaggctta tttcgtgg
//
LOCUS       DAOOBX010000201         1826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_70458_length_1826_cov_2.452287, whole genome shotgun sequence.
ACCESSION   DAOOBX010000201 DAOOBX010000000
VERSION     DAOOBX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70458_length_1826_cov_2.452287"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1826)
                     /locus_tag="PLY30_03395"
     CDS             complement(<1..>1826)
                     /locus_tag="PLY30_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPW65184.1"
                     /translation="AGKSLIGLAIAVMADVNLLMLPNQSLLSQLLANDREFFLKLAGG
                     TRVIVDGNALAREATDAFNRGERNSALMRVQELTNALNNKKGALVVMDRESAAQLQNT
                     LRKGEPAAYSKYLEAYENSNRLFDEVHQCANPMYFIVGGDRTPASLLSNYKVLDTVAQ
                     EAGKIVVFTEAEYRAGIDKDGSATKVLVTTDAKAFEQYKKDGIAAVYKENGKVTFWTG
                     EKIVKDVFMRNKAISESVMELKRAKGDNVTALWGSELFSILKSRAIDFRDGNAIITEL
                     LSNNGVALVDGQYRPVSAVGEKEVNQIIEDTAWLIGIAHTVKDAYGHEAKKLETRDRS
                     YDAIIAQNLRENMLLDTRSIELTSTVTAVSMFDLFVTRGTAETRGMSGTLMGVAIPIE
                     VMTGRSARAVARNGETGSFTAELFMNMRNDAANRFEINKGFGSENSTKKGSEGYFNFV
                     EKEINGLLKDSHRATIVGLETNYEIARLAEKLKADGRDVLVIDGSLTQAQVEGKVKEF
                     NDLVRANTQAGGNLSREIVILTNQRGLTGLDYQGRLNFMLFDPSLPEALLRQSGGRVG
                     RTIKDTVLAERYGLKVGETYESLFKLYLDRDYVAAQRSQLEGP"
BASE COUNT          369 a          558 c          483 g          416 t
ORIGIN      
        1 gggccttcca gctgactgcg ctgcgcggcg acataatcgc ggtccagata gagtttaaag
       61 aggctctcgt acgtttcacc gaccttgaga ccataccgct cagccagcac cgtgtccttg
      121 atcgtgcggc cgacacgacc gcccgactga cgaagcaggg cttcgggaag cgaaggatcg
      181 aacagcatga aattcaaacg gccctgataa tcgaggcccg tcaaccctcg ttggttcgta
      241 aggatcacga tttcccttga aaggttgccg ccggcctgag tgttcgcacg gacaaggtcg
      301 ttgaactcct tgaccttccc ctcgacctga gcctgagtga ggctgccgtc gatgacaaga
      361 acgtcccggc catccgcttt cagcttttcg gcaagacgcg cgatctcata gttcgtctca
      421 aggcccacga tcgtcgcgcg atggctgtcc ttgaggagtc cgttgatctc cttttcgaca
      481 aaattaaaat agccctcgga acctttcttg gtactgttct cgctgccgaa ccccttattg
      541 atctcgaaac ggttcgctgc atcattccgc atgttcatga aaagctcggc cgtgaacgag
      601 ccggtctcgc cgttcctggc gaccgcccgg gccgaacggc cggtcatcac ctcgatcggg
      661 atcgcgaccc ccatcaaggt cccggacatg ccgcgggtct cggcagtacc tcgggtcacg
      721 aaaagatcga acatgctcac cgcggtcacg gtcgaggtca actcgatcga cctcgtatca
      781 agaagcatgt tctccctcag gttctgcgcg atgatggcgt cgtaagagcg gtcgcgggtc
      841 tcgagcttct tcgcttcatg cccatacgcg tctttaacgg tatgagcgat accaatgagc
      901 caggccgtat cctcgatgat ctgattcact tctttctcgc ccaccgcgct gacgggacgg
      961 tactggccgt ccaccaaagc gacgccgttg ttcgacagca gttccgtgat gatagcgttc
     1021 ccgtccctga aatcgatcgc ccgggatttc aggatcgaaa aaagctcgga accccaaaga
     1081 gcggtcacgt tatcgccctt ggcacgttta agctccatca ccgattcgct aatggcttta
     1141 ttcctcatga aaacatcttt cacgatcttc tcgccggtcc agaaagtcac tttgccgttc
     1201 tctttataga ccgccgcgat cccgtccttc ttatattgtt cgaacgcctt cgcgtcggtc
     1261 gtaacgagaa ctttcgtggc gctcccgtcc ttatcaatac cggcgcggta ctccgcttcc
     1321 gtaaagacaa cgatcttccc ggcttcctga gcgaccgtat ccagcacttt gtagttcgaa
     1381 agaagcgacg cgggggtccg gtcgccgccg acgatgaaat acatcgggtt ggcgcactgg
     1441 tgaacttcgt caaacagacg attgctgttt tcataggctt ccaaatattt gctgtatgcg
     1501 gcgggctcac cctttcgcag ggtgttctga agttgggcgg cggattcgcg gtccatcacg
     1561 accagagcgc ccttcttatt attcaaagcg ttcgtcaact cctgaacgcg catcagagcg
     1621 gaattacgct cgccgcggtt gaaagcgtcc gtcgcttccc tggcaagggc gttcccatcc
     1681 acgatcacgc gggtcccgcc ggcaagcttc aggaagaact ccctgtcgtt ggcgagaagc
     1741 tggctcagca aggactggtt cgggagcatc agaaggttca cgtcggccat cacggcgatc
     1801 gcgagaccga tcagggattt cccggc
//
LOCUS       DAOOBX010000202         1815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_71015_length_1815_cov_2.360795, whole genome shotgun sequence.
ACCESSION   DAOOBX010000202 DAOOBX010000000
VERSION     DAOOBX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71015_length_1815_cov_2.360795"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1815
                     /locus_tag="PLY30_03400"
     CDS             <1..>1815
                     /locus_tag="PLY30_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65185.1"
                     /translation="AALTFIAMNNYDVALTELSVFMCTADASGTRLAEIGKEYGELAP
                     GKSAMIAVPVETRLKAGPVTHEVLFSARGPEGPVQVKRSVCSGIGPGFAERMPTLMWM
                     FAAPYQTLTDFGFTHATENVGFNFPLTKNSYIAESERKLDRALLSGVRFVKIFSPSKP
                     GGSSEGQYERQRRDGTFSDASQPVLEVSDPDMVAHAEKVMEGNAKAFADHPAFVGILP
                     CSEHRDHTFPSFNTEAQRYKAETGRNVPDEVSGKTLKRSLAEKRYPDGVVPTDDPILA
                     YYRWFWKGGDGWPKFISAYADTARKVIKRPFFSFFDPAVRCPPVWGSGGSVDYLSQWI
                     YATPEPMNVAGPLEELFAMARGRPGQKVMIMTQFICYRSQIAPENVKVDPEPDWVQRR
                     PNAKFPTIPPDSLQEATWSMLAKPVKGIMYHGWGTIYEAGEETGYCYTNPEAAERLRC
                     LLKEVVAPLGPMLMKLGRDDSPVVILESATTALLGGPASWGWSAPAITFFQRARLDPR
                     VIYEEQVMRDGFGNARIVYAPQCRFMTPDMIAKIRAFQSQGGVLVGDNEMLKALSPDV
                     TVPVVSFKAPPKSDHTEDVEAMEAAQNGDGEKRAGTVRA"
BASE COUNT          364 a          500 c          582 g          369 t
ORIGIN      
        1 cgcggcgctt acgttcatcg cgatgaataa ttatgatgtc gcgttgaccg aactctccgt
       61 tttcatgtgt acggcagacg cttccgggac acggctggcg gagatcggga aagaatacgg
      121 agagcttgct cccggcaaat cggcaatgat cgcggttccg gtggagacgc ggttaaaggc
      181 gggaccggta acgcacgagg tgctgttttc agcgcggggt ccagaaggcc cggttcaagt
      241 caagcggagc gtctgcagtg ggatcggtcc tggttttgcc gagcgtatgc cgacactcat
      301 gtggatgttt gcggcgcctt atcagaccct cacggacttc gggtttacgc atgcgactga
      361 aaatgtgggc ttcaactttc cattgacgaa aaacagctac atcgccgagt cggaacgcaa
      421 gcttgatcgc gccctgctct ctggtgtccg ttttgttaag attttcagtc cgtccaagcc
      481 gggcggatcg tccgagggcc aatacgaaag gcaacgccgt gacggcacct tttccgatgc
      541 ctcgcaaccg gtgctggagg tgtccgatcc ggacatggtc gctcacgctg aaaaagtcat
      601 ggaggggaat gcgaaggctt ttgccgacca tcccgccttt gtcggaatcc tgccgtgttc
      661 agaacatcgc gatcacacct tcccgtcctt caataccgag gcgcaacgct ataaagcgga
      721 gacggggcgc aacgtgcccg acgaggtttc cggcaagacg ctgaagcgga gtctcgccga
      781 aaagcgttat ccggacggcg tggtgccgac ggacgatccg attctcgcct attaccgctg
      841 gttctggaag ggcggggacg gctggccgaa gtttatcagc gcttatgcgg ataccgccag
      901 gaaagtgatc aaacgtccct tcttcagctt cttcgatccg gcggtccggt gtccgccggt
      961 ctgggggtcc ggcggcagtg tcgactacct tagccagtgg atctatgcaa cgcctgaacc
     1021 gatgaacgtg gcggggcctt tggaagagct gttcgcgatg gcgcggggac gtccgggaca
     1081 gaaagtcatg atcatgaccc agttcatttg ttaccgctcg cagatcgctc cagagaacgt
     1141 gaaggtcgat cctgagccgg actgggttca gcggcgccct aacgcgaagt ttccgacgat
     1201 tccgccggat tccttacagg aggcgacttg gtcgatgctt gcgaaaccgg tgaagggcat
     1261 catgtatcat ggctggggga cgatttacga agcaggcgag gaaacgggct actgctacac
     1321 caatcccgaa gccgctgaac gactccgctg tcttctcaag gaggtcgtgg cgccgctcgg
     1381 tccgatgctg atgaagcttg ggcgggacga ttcgccggtt gtgattctcg aaagtgcgac
     1441 gaccgcgctg ctgggaggtc cggcttcgtg gggatggtcc gcgccggcga tcacgttctt
     1501 ccagcgcgca aggctcgacc cgcgggtcat ttacgaagaa caggttatgc gtgacggctt
     1561 cggaaacgcc aggattgtct acgcgccgca gtgccgcttc atgacgccgg acatgatcgc
     1621 gaagatacgc gcgttccagt ctcaaggcgg cgtactcgtc ggcgacaacg aaatgctgaa
     1681 ggcgttgtcg ccggatgtca ccgtgccggt cgtcagtttc aaagcgccgc ccaagtctga
     1741 tcacaccgag gatgtcgagg ctatggaggc cgcacagaac ggcgatggcg aaaaacgcgc
     1801 ggggacggtg cgggc
//
LOCUS       DAOOBX010000203         1794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_72126_length_1794_cov_3.266245, whole genome shotgun sequence.
ACCESSION   DAOOBX010000203 DAOOBX010000000
VERSION     DAOOBX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72126_length_1794_cov_3.266245"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PLY30_03405"
     CDS             <1..838
                     /locus_tag="PLY30_03405"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPW65186.1"
                     /translation="GTILGTSRTNPYKKAEDVEKVRETIKKNRLDALIAIGGEDTLGA
                     AHKLFKDGLPVIGVPKTIDNDLNATDYTFGYDTALNIATECLDRLHTTADSHDRVMVV
                     EIMGRHAGWIALEAGLAGGADAILIPEVPMTADEICEYIQSRHKRGKNFSIVAVAEGF
                     SIKGEDLVTSKKELDAFGHVQLGGVGDRLAKLIEKKTGFETRCTVLGHIQRGGSPTAF
                     DRVLGTRFGVKAVELVKLGKFGHMAALRGREIIEVPLADAVGALKTVPMHLYETAKMF
                     FG"
     gene            842..1414
                     /locus_tag="PLY30_03410"
     CDS             842..1414
                     /locus_tag="PLY30_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HPW65187.1"
                     /translation="MRNYFDAVLAGHADAFQKTFVPEQLAVIDQIVRAIIDMLKGGHK
                     LLLCGNGGSGTDALHIAAEFVGRFEKERISLPAIALTADTASITAIGNDYGYDRVFER
                     QVEGLGQKGDVLFALSTSGKSPNVLKAIEKAKRKGILTVGFTGKDGGAMKDAVDLLFL
                     VRSNKTAHIQEMHMVALHGICETVEGILFG"
     gene            1422..>1794
                     /locus_tag="PLY30_03415"
     CDS             1422..>1794
                     /locus_tag="PLY30_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HPW65188.1"
                     /translation="MDKKILTPRKLAATVARLKSQGKKIVFTNGTFDILHLGHVTYLR
                     KARREGDVLIVGVNTDRSVKKYKDPSRPVNPEGDRIRVLTALACVDYAILFGDTTPLK
                     LILKIRPDVLVKGADWALRDIV"
BASE COUNT          389 a          504 c          543 g          358 t
ORIGIN      
        1 aggaacgatc ctcgggacct cccgcacgaa tccttacaag aaagcggagg acgttgagaa
       61 ggttcgcgaa acgatcaaga agaaccggct tgatgcgctc atcgcaatcg gcggtgagga
      121 cactcttggc gcggcacaca agctctttaa ggacggtctt cccgtgatcg gagttccgaa
      181 aacgatcgac aacgatctca acgccaccga ctacacgttc ggttacgaca cggcgctcaa
      241 tatcgccacg gaatgtcttg accgcctcca cacgacggcg gattcgcacg accgcgtcat
      301 ggtggtcgag atcatgggcc gtcacgccgg atggattgcc ctcgaagcgg ggctcgcggg
      361 cggggcggac gcaatcctga tccccgaggt cccgatgacc gcggatgaga tttgcgaata
      421 tatccagagc cggcataagc gcggcaagaa tttcagcatt gtcgcggtcg cggaaggctt
      481 ttccattaag ggcgaggacc ttgtcacgag caaaaaggaa ctggacgctt tcggtcacgt
      541 tcagctcggc ggcgtcgggg accggctcgc gaaactcatc gaaaagaaga ccggtttcga
      601 gactcgctgc acggtgctcg gtcatatcca gcgcggtggc tcgccgactg cttttgatcg
      661 ggttctcggg acgcgtttcg gcgtcaaggc ggttgagctc gtcaagctcg gaaaattcgg
      721 tcacatggcg gcgcttcgcg gccgtgagat tatcgaggtt ccgctcgcgg atgccgtggg
      781 cgccctcaaa acggtgccga tgcaccttta cgaaaccgcc aagatgttct tcggctgatc
      841 catgcggaac tatttcgacg cggtcctcgc cggacacgcc gatgccttcc agaagacgtt
      901 cgttcccgaa cagcttgcgg tgattgacca gatcgtccgg gcgattatcg atatgctcaa
      961 aggcggtcat aagctgcttc tgtgcggcaa cgggggttcc gggaccgatg cgctccatat
     1021 cgcggccgag ttcgtgggcc gctttgaaaa agagcgcatc agtcttcccg cgatcgccct
     1081 taccgcggac acggcgtcca tcaccgcgat cggtaacgat tacggttatg accgcgtttt
     1141 cgaaagacag gtcgaggggc tcggtcaaaa aggggacgtc ctgttcgctc tttcaacgag
     1201 cggaaagtcc cccaatgtgc tgaaggcgat cgagaaggcg aaaagaaagg ggatcctgac
     1261 ggtcggtttc accggcaagg acggcggggc gatgaaggac gccgttgacc tgctattcct
     1321 cgtccgttcg aacaagaccg cgcacattca ggaaatgcac atggtcgcgc tccacgggat
     1381 ttgcgagacg gtagaaggta tcctcttcgg ataaccggac catggataag aagattctca
     1441 cgccccgaaa gctggccgct acagtcgcgc gcctcaaatc gcaggggaag aagatcgttt
     1501 tcacgaatgg gacctttgat atcctgcacc ttggtcatgt cacctacctc cggaaagcgc
     1561 gccgtgaagg agatgtcctg atcgtcggtg ttaataccga ccgttctgtg aaaaagtaca
     1621 aggatccgag ccgccccgtg aatcctgaag gcgaccggat cagggtgctg acggcgctcg
     1681 cttgcgtgga ctatgcgatt ctttttggtg atactacgcc gctcaagctg atcctgaaga
     1741 tccgccccga tgttctcgtc aaaggcgcgg actgggcgct cagggatatc gtcg
//
LOCUS       DAOOBX010000204         1793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_72168_length_1793_cov_3.882048, whole genome shotgun sequence.
ACCESSION   DAOOBX010000204 DAOOBX010000000
VERSION     DAOOBX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72168_length_1793_cov_3.882048"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLY30_03420"
     CDS             complement(<1..228)
                     /locus_tag="PLY30_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65189.1"
                     /translation="MDLIRFTLKEGVEFAKKEGAQPLLDAFTKRAAELNPGINAFLDF
                     DNHIQPRRLDKSNLRGWSGSLYGAPVSSKDNI"
     gene            complement(289..573)
                     /gene="gatC"
                     /locus_tag="PLY30_03425"
     CDS             complement(289..573)
                     /gene="gatC"
                     /locus_tag="PLY30_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPW65190.1"
                     /translation="MEFNIDEVAQLARLNLKAEEKARLERDLGAILDYVKNLEKLDTE
                     NVEPTSHVLDLENVCRKDEARSTDAATKALEHAPCADGRFFKVPKIVQRD"
     gene            complement(752..1306)
                     /locus_tag="PLY30_03430"
     CDS             complement(752..1306)
                     /locus_tag="PLY30_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPW65191.1"
                     /translation="MSFTDLVLKRRSVRRFSDKPVKREDILACLEAARLAPSACNSQP
                     WKFIVVDDPGKRARLAGRIFSGLYSMNEFAKNAPVIIAVVSEKMKFLATVGAQVRKTE
                     YYLTDIGIAGEHFVLRARELGLGTCWVGWFDEKALKEELGVPKDRKVDVVIALGYPAE
                     DQVVPKNRKSLAEIGAFNRYDGAL"
     gene            1459..1626
                     /locus_tag="PLY30_03435"
     CDS             1459..1626
                     /locus_tag="PLY30_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65192.1"
                     /translation="MKKYGNPHRASRYYRHYKWRGSSFGEEIILDYFQDSEKMKNTTE
                     PVFDAGKAYRP"
BASE COUNT          365 a          549 c          502 g          377 t
ORIGIN      
        1 gatattgtcc ttgctggaga ccggagcgcc gtacagtgaa ccgctccaac ctcgcaggtt
       61 ggatttatcc aacctgcgag gttggatatg gttgtcaaaa tccaggaaag cgttgatccc
      121 gggattcagt tcagcggcgc gtttcgtgaa cgcgtcgagc agaggctggg cgccctcttt
      181 tttcgcgaac tcgacccctt ctttgagagt aaacctgatc agatccattt cggctcatga
      241 cgacccgcga ggccggataa atccggcctc gcgggtcggt tgcggttatt aatcgcgttg
      301 cactattttc gggactttga agaaacggcc gtcggcgcac ggagcatgtt caagggcctt
      361 cgtggccgcg tccgtggacc gcgcctcgtc cttccggcaa acgttttcga gatcaaggac
      421 atggctcgtc ggctcgacgt tttccgtgtc gagtttttcg aggttcttca catagtcgag
      481 gatcgccccg aggtctcttt cgagcctggc cttctcttcg gccttcagat tgagccgcgc
      541 aagctgcgcg acctcgtcga tattgaattc cataagttta ccccgttaga gatgggcctc
      601 gcccggagag cgcgaccgtg tgccttcccg gttatcatta cattcccgat caccccgcct
      661 gaccaatccc gcaaggaatc actacgcgat gatctctgac ggggcgggac attctgcccg
      721 aagggacgtg gcgtgtcaac gaagcggagg gtcagagcgc gccgtcatag cggttgaacg
      781 cgccgatctc cgcgagagac ttccggttct tcggaacaac ctggtcctcg gccgggtacc
      841 cgagcgcgat cacgacgtcg accttgcggt ccttcgggac gccgagctcc tccttcaggg
      901 ccttctcatc gaaccagccg acccagcagg tcccgaggcc gagctcccgg gcccgcagga
      961 caaagtgctc tcccgcgatt ccgatatcgg tcaggtaata ttcggtcttc cggacctgcg
     1021 ccccgacggt ggcgagaaac ttcatctttt ccgaaaccac cgcgataatc acgggcgcgt
     1081 tcttcgcgaa ctcgttcatg gaataaagtc ccgaaaagat tctccccgcg agccgcgcgc
     1141 gtttcccggg atcatccacc acaataaatt tccagggctg ggaattgcac gccgacggcg
     1201 cgaggcgggc cgcctcgagg caggcaagaa tatcctcccg cttgacgggt ttatcggaaa
     1261 atcgccggac gcttcttcgc ttcaggacaa gatcagtaaa ggacatgccg tatctccttg
     1321 agggaagtga ttctacaccc tgacctcaaa agggctaccg ttaatttcaa gcccccggaa
     1381 gccggggctc aaacgggccc caaaaccgct gttatctatt attcctgaaa aagaggcgac
     1441 aagatccgcg acgcgctcat gaaaaagtac gggaatccgc acagggcctc gcgatattac
     1501 cggcattaca aatggcgcgg ctcctcgttc ggcgaggaga ttatcctgga ttattttcag
     1561 gattcggaga agatgaagaa cacgaccgaa ccggtcttcg acgccggcaa ggcatatcgc
     1621 ccctaaccgg ccgcggcgtt cccgcccatc cctttcacga catactccag cacgacatcc
     1681 gcctgtttgg ccgccgcgat ggcgacgcgc ggcgcgagcg ccggcgttct ccggatgtcg
     1741 gagcgaagat ccccgatcat cacgaggttc tccctgatgc gatggatgcg aat
//
LOCUS       DAOOBX010000205         1789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_72407_length_1789_cov_2.968281, whole genome shotgun sequence.
ACCESSION   DAOOBX010000205 DAOOBX010000000
VERSION     DAOOBX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72407_length_1789_cov_2.968281"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..543)
                     /gene="nuoK"
                     /locus_tag="PLY30_03440"
     CDS             complement(232..543)
                     /gene="nuoK"
                     /locus_tag="PLY30_03440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPW65193.1"
                     /translation="MIIPLPHVLLFSLALFGIGAYGVLTRRNIIGILISIELMLNAAG
                     INLIAFSKTTALAPETGQIFAVFVIAIAAMTATVGLAIVLALYRARKTVFADEVNLLK
                     W"
     gene            complement(540..1055)
                     /locus_tag="PLY30_03445"
     CDS             complement(540..1055)
                     /locus_tag="PLY30_03445"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPW65194.1"
                     /translation="MDILNFGIKVGIYALMAGTLVLAVGVVTLRNLFHSALCLAATLI
                     GTAALYLVFHAEFLAAVQILLYVGAVVTVIIFAIMLTEHIAERSVAQRNRQSLPALLG
                     ILVLIAVTGRILLKTPWAVNPATVGARVTVADLGAALMGTYVFPFELVSVILIAALIG
                     AVIVAKKEKSS"
     gene            complement(1115..>1789)
                     /locus_tag="PLY30_03450"
     CDS             complement(1115..>1789)
                     /locus_tag="PLY30_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPW65195.1"
                     /translation="KYSMIGGMRTAAQMISYEVPMTLAVVPVIMMSQSLSTASIVNAQ
                     EGLRWFVFTPWGLAGFVIFFICTVAECNRPPFDLPEAESELVAGFHTEYSGMKFALFY
                     MAEYMNAFTLSAIAVTLFLGGWQGPFLPSWVWFFTKTFALIFAMIWFRATFPRFRVDQ
                     LMGIAWKLLLPLTLVNMLVAGLWYFIPGIAGWVGAAVILAVSFLGLTALNKPVIPEKR
                     VYLLEN"
BASE COUNT          430 a          485 c          559 g          315 t
ORIGIN      
        1 gcacaggccg cggcgcttgt cgcgatcgaa agccaggaag cgcggtgttt caggaaacgg
       61 gccccgagga gaatgttgac gacgaaggcc gtgaacggga gcaggggcac aaggtagatc
      121 atgtcagggt tcataacgag ctccctgaat ccgaattttt aaatccgaat aacgaacgaa
      181 acttcccggg gtttccggat cttgatgtga gatatttctt gtccacaggc gttaccattt
      241 cagtaaattg acctcatcgg caaagaccgt cttccgcgcg cggtaaaggg ccaggacgat
      301 cgcgaggccc acggttgcgg tcattgcggc gatcgcgatc acgaacaccg cgaaaatttg
      361 ccccgtttcg ggcgcgagag cggtcgtttt ggagaatgcg atcaggttga tgccggccgc
      421 gttcagcatg agttcgatcg agatcaggat tccgatgata ttgcgtcgcg tcaggacgcc
      481 gtaagcgccg atcccgaaga gcgcgagaga aaaaagaagc acgtgcggaa gaggaataat
      541 catgacgatt tttccttctt cgcgacgatc acggcgccga tcagggccgc gatcaggatg
      601 accgaaacaa gttcgaaggg gaacacgtaa gtgcccatga gcgcggcgcc gaggtcggcc
      661 acggtgactc tcgctccgac cgttgccggg ttcaccgccc acggggtctt gaggagaatc
      721 ctgccggtca cggcgataag cacgagaatg ccgaggagcg ccggaaggct ctgccggttg
      781 cgctgggcca cggatcgctc cgcgatgtgt tcggtgagca tgatcgcgaa gatgatgacg
      841 gtgacgactg cgccgacgta gagcaggatc tggaccgccg cgaggaactc ggcgtggaaa
      901 accagataga gtgccgcggt cccgatcagg gtggcggcga gacagagcgc cgaatgaaaa
      961 aggtttctga gcgtgaccac gccgaccgcg agcacgagcg tcccggccat cagcgcgtaa
     1021 atcccgacct tgatcccaaa attcagtatg tccattttta actctctttt acagcggttg
     1081 ccggaaacgg ttcctttaaa aaagcgtttt cgagctagtt ttccagcaga taaacccgtt
     1141 tttcgggaat gaccggtttg ttcaacgcgg tcaacccgag gaacgaaacg gccaggatga
     1201 cggcggcgcc gacccagccg gcgatccccg gaatgaaata ccagagtcct gccacgagca
     1261 tgttcacgag cgtaagcggc aggagaagtt tccacgcgat ccccatcagc tgatccaccc
     1321 ggaagcgggg gaacgtcgcg cggaaccaga tcatcgcgaa aataagggcg aaggtcttcg
     1381 tgaagaacca gacccacgag ggcaggaacg gtccctgcca cccgccgaga aagagcgtca
     1441 cggcgatcgc ggaaagtgtg aaagcgttca tgtactcggc catataaaaa agggcgaact
     1501 tcataccgct gtattcggtg tggaaacccg ccacgagctc ggactcggct tcggggaggt
     1561 cgaacggcgg gcggttgcat tcggcgaccg tgcagatgaa aaagatcacg aagccagcaa
     1621 gaccccacgg cgtgaagaca aaccagcgga ggccttcctg cgcgttcacg atcgacgcgg
     1681 ttgagaggct ctggctcatc atgatgaccg ggacgaccgc aagcgtcatc gggacctcgt
     1741 aggagatcat ctgggcggcg gtacgcatgc ccccgatcat ggaatattt
//
LOCUS       DAOOBX010000206         1777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_73075_length_1777_cov_2.807782, whole genome shotgun sequence.
ACCESSION   DAOOBX010000206 DAOOBX010000000
VERSION     DAOOBX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73075_length_1777_cov_2.807782"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..419)
                     /gene="rpsM"
                     /locus_tag="PLY30_03455"
     CDS             complement(36..419)
                     /gene="rpsM"
                     /locus_tag="PLY30_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPW65196.1"
                     /translation="MPRILGVDIPREKKIEVALTYLYGIGRTLARQVLDEAGVDWNKR
                     AKDLTEADVSTITGIIQKTCRVEGDLRREVQQNIKRLMDIRCYRGVRHVKGLPVRGQR
                     THTNARTRKGKRKTVGGLSKKAPAP"
     gene            complement(445..558)
                     /gene="rpmJ"
                     /locus_tag="PLY30_03460"
     CDS             complement(445..558)
                     /gene="rpmJ"
                     /locus_tag="PLY30_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPW65197.1"
                     /translation="MKVRSSVKRICENCKIVRRKGYVYVICSNPKHKQRQG"
     gene            complement(574..792)
                     /gene="infA"
                     /locus_tag="PLY30_03465"
     CDS             complement(574..792)
                     /gene="infA"
                     /locus_tag="PLY30_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPW65198.1"
                     /translation="MPKEDAIEVEGTVVEPLPNAMFRVQLENGHRVLAHISGKMRMHY
                     IRILPGDKVKVELSPYDLTRGRITFRVK"
     gene            complement(792..1550)
                     /gene="map"
                     /locus_tag="PLY30_03470"
     CDS             complement(792..1550)
                     /gene="map"
                     /locus_tag="PLY30_03470"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPW65199.1"
                     /translation="MVELKSGREIRIIGESGKILQKVFRALRPMMKQGVSGLDLDQAA
                     EKVIRENGAQASFRGYRGYPATICLSLNEEVVHGIPDRRKLMNGDVVSVDVGAKWKGF
                     HSDAARTWTVGGVDDHRQKLIAVTKDSFDAALAAYKPGWRIGDISEAVQKYVESHGFQ
                     VVREYAGHGIGRELHEDPQVPNYGKAGRGTKIEPGLVLAIEPMVTAGHYAVETLANGW
                     TVVTKDRKCASHYEDTVAFTEDGFINLTRDEATE"
     gene            complement(1557..>1777)
                     /locus_tag="PLY30_03475"
     CDS             complement(1557..>1777)
                     /locus_tag="PLY30_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPW65200.1"
                     /translation="RKEGVCDCHGQPLVTRKDDQPDTIRKRLAVYHESTKPLIEYYAK
                     NDLLRNVPADGDAPAVQKLLEKLFETIR"
BASE COUNT          308 a          577 c          448 g          444 t
ORIGIN      
        1 ccttttcttt tacaagtgct tcggacattt ctttattacg gagccggcgc cttcttgctg
       61 agtccgccga cggtcttgcg tttgcccttg cgggtacggg cgttcgtatg cgtccgctgc
      121 ccccggaccg gcaggccctt aacgtgacgg acaccccggt aacagcggat atccatcaaa
      181 cgtttgatgt tctgctgcac ttcgcggcgc agatcaccct cgacccggca ggtcttctga
      241 atgataccgg tgatcgtcga cacatcggcc tcggtcaggt ccttggcgcg tttgttccaa
      301 tccacgccgg cttcgtccag cacctgcctc gcgagggtac ggccgatccc gtacaggtac
      361 gtgagggcca cctcgatctt tttttctctc ggaatatcaa cacccagaat acgcggcata
      421 acttttagtt cctttcctgg ttcttcaacc ctggcgttgt ttgtgtttcg ggttggaaca
      481 gatcacatag acgtagcctt tgcggcgtac gattttgcaa ttttcacaga ttctcttgac
      541 tgaactcctg accttcatgg catcgctccg ttcttatttg actctgaaag tgatcctgcc
      601 ccgggtcagg tcgtaaggcg acagctcgac cttgacttta tcccccggaa ggatgcggat
      661 gtagtgcatc ctcatctttc ccgaaatatg cgcaagcacc cggtgtccgt tctcaagctg
      721 aacccggaac atcgcattcg gaaggggctc gaccaccgtc ccttccactt cgatcgcatc
      781 ttctttcggc attactccgt cgcctcgtct ctggttaaat ttataaaacc atcctcggtg
      841 aacgccaccg tgtcttcgta gtggctcgcg catttccggt ccttcgtaac gaccgtccag
      901 ccgttcgcga gcgtctccac cgcgtaatgc cccgcggtga ccatcggttc gatcgcgagc
      961 acaagcccgg gttcgatctt cgtgccccgt ccggctttcc cgtaattcgg gacctgcggg
     1021 tcttcgtgaa gctcccgtcc gatcccgtgt cccgcatact cccgcacaac ctggaacccg
     1081 tgagactcga cgtatttctg aaccgcttcc gagatgtctc caatgcgcca gcccggcttg
     1141 tacgcggcaa gggccgcgtc gaaagaatcc ttcgtgaccg cgatcagttt ctgccgatgg
     1201 tcatcgaccc cgccgaccgt ccaggtcctc gcggcgtccg aatggaaccc cttccatttc
     1261 gcgccgacgt cgacgctcac aacatcgccg ttcatcagct tccgcctgtc gggaatcccg
     1321 tgaaccacct cttcgttgag agaaaggcag atcgtcgccg ggtaaccccg gtatcccctg
     1381 aatgacgcct gggcgccatt ctcgcgaatc accttttcag cggcctggtc gagatcgagt
     1441 ccgctcacgc cctgcttcat catgggcctg agcgccctga aaaccttctg aagtattttc
     1501 cccgattccc cgataatccg gatttcccgt cctgatttca gttcgaccat gcggaatcag
     1561 cggatcgttt caaagagctt ctctaacaat ttctgcaccg ccggcgcatc gccgtcggcc
     1621 ggaacgttcc ggagaaggtc gtttttcgcg tagtactcga tcagcggctt cgtggattcg
     1681 tgatacacgg caagccgctt gcggatcgtg tccggctgat cgtccttgcg ggtcacgagc
     1741 ggctgcccgt ggcaatcgca gacaccttcc ttcctgg
//
LOCUS       DAOOBX010000207         1757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_74183_length_1757_cov_3.247944, whole genome shotgun sequence.
ACCESSION   DAOOBX010000207 DAOOBX010000000
VERSION     DAOOBX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74183_length_1757_cov_3.247944"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PLY30_03480"
     CDS             <1..1112
                     /locus_tag="PLY30_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HPW65201.1"
                     /translation="DEIALVPGDVTINPEEVEIGLPLGDRTLPIPFLASAMDGVVDPK
                     FAVAMGKLGGLAVLNLEGIYTRYDDYGAILDEISRATPEKATQLVQKLYVKPINEKLI
                     AKRIREIKKAKVPCAVSAIPQRSERFGKIAEEAGADFFVVQSTVTSVRHISRAYQVVD
                     FKKFCRKMKIPVILGNCVTYKVALDLAETGAAGLLVGIGPGSACTTRGVLGVGVPQVT
                     AIADCAAAREFYYKKTGRYVSIIADGGMSTGGDICKSIASGADGVMVGSAFARAKEAP
                     GRGFHWGMATPHANLPRGTRIHVGTTGSLDEILYGPARLDDGTQNLVGALRTSMGNVG
                     AKNIREMQLTEIVIAPAIKTEGKLFQSAQKLGMCK"
     gene            1188..>1757
                     /gene="guaA"
                     /locus_tag="PLY30_03485"
     CDS             1188..>1757
                     /gene="guaA"
                     /locus_tag="PLY30_03485"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPW65202.1"
                     /translation="MDQTIVVLDFGSQYTQLIARRVREAKVFSKVYPFYAPFEEVMKD
                     NPKGIIFSGSPANVYAKNAPIPDNRFFYSGIPILGICYGMQLLSHKLGGRVHKSRKRE
                     YGFSKLHISKRASDLFEGLPKTVTCWMSHGDKLDRIPQGFERLAYTENAPFAAMGDAK
                     KKIYAIQFHAEVAHTELGAKILENFLVRVC"
BASE COUNT          369 a          500 c          535 g          353 t
ORIGIN      
        1 ttgatgagat cgcccttgtt cccggggatg tgacgatcaa tcccgaggaa gtcgagatcg
       61 gcttgccgct cggggacagg acgctcccca taccgtttct tgcctccgcc atggatggcg
      121 ttgtggaccc gaaattcgcg gtcgcgatgg ggaagctcgg cggtctcgcg gtgctgaacc
      181 tcgagggaat ctacacccgc tacgacgatt acggggcgat cctggacgag atctccagag
      241 cgacgccgga aaaggcgacg caacttgtgc agaagctcta cgtcaagccg atcaacgaaa
      301 agctgatcgc caagcgtatc cgtgagatca aaaaagcgaa ggtgccctgc gccgtttccg
      361 cgatcccgca gcgttcggag cgtttcggaa agatcgccga ggaagcgggc gcggatttct
      421 tcgttgtcca gtccaccgtg acgagtgtcc gtcatatctc gagggcatac caggtcgtgg
      481 atttcaagaa attctgcagg aagatgaaga ttcccgtgat cctcggaaat tgcgtgacct
      541 acaaggtcgc cctcgacctg gcggagaccg gcgccgcggg gcttcttgtc ggtatcggac
      601 ccggttcggc atgcacgacg cgcggagtgc tcggggtcgg cgtgcctcag gtgaccgcaa
      661 tcgcggactg cgccgcggcc cgcgagttct attacaagaa gaccggccgt tacgtttcga
      721 tcatcgcgga cggcggcatg tctaccggcg gcgatatctg caaatccatc gcgagcgggg
      781 cagacggtgt tatggtcggt tccgcgttcg cgcgcgcgaa ggaagcgccc ggtcgcggtt
      841 ttcactgggg catggcgacg ccgcacgcga accttccgcg tggcacacgc atccacgtcg
      901 gaacgacggg ttctctcgat gagattctct acgggcccgc gcgcctcgat gacgggacgc
      961 agaacctggt cggagcgctc cggacttcga tggggaacgt cggcgcgaag aatatccgcg
     1021 agatgcaact gaccgagatt gtgatcgccc ccgcgatcaa gaccgaaggg aaactttttc
     1081 agtccgctca aaaacttgga atgtgtaaat aggagactgg tatggagtat ggggtacgga
     1141 gacaggattt aactccgtgc tcaaggctcc ccccaccgcg ctgaattatg gatcaaacta
     1201 ttgtcgttct cgattttggc tcacagtaca cccagctgat cgcccgccgc gtgcgcgagg
     1261 cgaaggtttt ttccaaggtc tatccctttt acgcgccgtt cgaggaggtg atgaaggata
     1321 atccgaaagg gatcatcttt tcgggctctc ctgcgaatgt ctacgcaaag aacgcgccga
     1381 tcccggacaa tcggttcttc tatagcggta ttcccatcct cggtatctgt tacggcatgc
     1441 agcttctttc ccacaagctc ggcggccggg tccataaatc caggaaacgg gaatacgggt
     1501 tctcgaaact ccacatctca aaacgcgcga gcgatctttt cgaggggctc ccgaaaacgg
     1561 tcacttgctg gatgagccat ggcgacaagc tggacaggat tccgcaaggg ttcgaacgcc
     1621 tggcctacac cgagaacgcg ccgttcgccg cgatggggga cgcgaaaaag aagatctacg
     1681 cgatccagtt ccacgcggag gtcgctcaca cggagcttgg cgcgaaaatc ctcgagaatt
     1741 tccttgtgcg cgtttgc
//
LOCUS       DAOOBX010000208         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_75188_length_1739_cov_3.309976, whole genome shotgun sequence.
ACCESSION   DAOOBX010000208 DAOOBX010000000
VERSION     DAOOBX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75188_length_1739_cov_3.309976"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLY30_03490"
     CDS             <1..284
                     /locus_tag="PLY30_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65203.1"
                     /translation="VHIATVVGCPVVSIFGRHEPGLGPDRWKPLGTKCRVVAKDVSDI
                     PASERKFTYIEDITVDEVHRAVSDLLHPRELDPGSSSRGWRGVEGGGLK"
     gene            281..>1181
                     /locus_tag="PLY30_03495"
     CDS             281..>1181
                     /locus_tag="PLY30_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPW65204.1"
                     /translation="MRTLRRVLVVHPYGIGDLLFVTPVLRALRLLPAVETVDLLIGSR
                     TLEVVEHNPHVNGIFVLDKDKLRRQTRTRKFRELLDLGMKLRAKRYDLLLDFSLRPEN
                     AFWSLAFLGIPKRAGFAYKRRAVFHNVRYPLPEGYWKQHAADFYAGLAEAAGIPVADR
                     FLEYYFPPEKAELEKKMAQRTAALGRKFITVSPGGGDSWGREADFKRWPVGNYAVLIG
                     MMQKKYGFEGVCIVGSKAETELCGQLKNALGLPVVVLAGETTIAETAVLLQKSSLFVG
                     NDGGLVHLARALEVPLVAFYGPVP"
     assembly_gap    1182..1191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1192..1386
                     /locus_tag="PLY30_03500"
     CDS             <1192..1386
                     /locus_tag="PLY30_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65205.1"
                     /translation="VYGPYPVSPDAAAVFKQDLACRPCYYKFRFKKDCPTIACLKTLA
                     PEEALEQIERVFSGGFPQKG"
     gene            1391..>1739
                     /locus_tag="PLY30_03505"
     CDS             1391..>1739
                     /locus_tag="PLY30_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65206.1"
                     /translation="MAKRKVIEIDRKKHNGRGLCATSGAEGTLVPDQGKGAALAQEIF
                     RDGMGACGGTRRSYPSMKAAYPAGVRRSSPANTGRRWGALFLAMFLSANVHAAKPEKI
                     FPPSGKPPARFFVG"
BASE COUNT          326 a          488 c          557 g          358 t
ORIGIN      
        1 ccgtccatat cgcgacggtg gtcggttgtc ccgttgtctc gatcttcggg cgccatgaac
       61 ccggtcttgg gcccgaccgc tggaagcctc tcggtacaaa atgcagggtt gtggcgaaag
      121 atgtgagcga tatccccgcg tccgaaagaa aattcactta catcgaagac atcacggtgg
      181 acgaagttca tcgtgccgtt tccgacctgc tccacccccg ggagctagac cccgggtcta
      241 gctcccgggg gtggaggggt gtggaaggcg gaggtctgaa atgaggaccc tgcggcgcgt
      301 tctggtcgtt cacccgtacg ggatcgggga ccttttgttc gtgacgccgg tcctgcgcgc
      361 cctccgactg ctcccggcgg tcgagactgt cgatctcctg atcgggtccc ggaccctcga
      421 agtggtggag cacaacccgc acgtgaacgg gatcttcgtg ctcgacaagg acaagctccg
      481 ccgccagacc cgtacccgga aattccggga gctcctggac ctcgggatga agcttcgggc
      541 gaaacggtat gacctcctgc tcgatttttc gctgaggcct gaaaacgctt tttggtcgct
      601 cgccttcctc ggtattccga agcgcgcggg gtttgcgtac aagcgccgcg cggtttttca
      661 taatgttcgc tatccgcttc ccgaaggtta ttggaaacag catgccgcgg atttttacgc
      721 gggtctcgcg gaagcggcgg gaatcccggt cgcggaccgg ttccttgaat attattttcc
      781 gccggagaag gcggagcttg aaaagaaaat ggcgcaacgg acggccgcgc ttggccgcaa
      841 attcatcacg gtcagtcccg gcggaggcga ctcatggggg cgggaagcgg atttcaagcg
      901 gtggcctgtc gggaattatg cggtcctgat cgggatgatg cagaaaaagt acggatttga
      961 gggtgtgtgc attgtgggaa gcaaggctga gaccgaattg tgcggacagc tcaagaatgc
     1021 gctcggtctg ccggttgtcg tcctggcggg ggagacgacg atcgcggaaa cagcggtcct
     1081 ccttcaaaag tcctcgcttt ttgtcgggaa cgacggcggg ctggtccatc tggcccgcgc
     1141 ccttgaggtg cccctggtcg ctttttacgg tcccgtgccg cnnnnnnnnn ngtgtacgga
     1201 ccttatcccg tttccccgga tgcggcggcg gtctttaaac aggaccttgc ctgccgtccc
     1261 tgctattata aattccgttt caaaaaggat tgtccgacca ttgcttgcct gaaaacgctt
     1321 gcccccgaag aagcgcttga acagatcgag cgggtattta gcggggggtt cccgcaaaag
     1381 ggttgagatc atggcgaaac gaaaggtcat cgaaatcgac agaaaaaaac acaacggacg
     1441 cggactttgt gccacgtccg gcgcggaagg gacgctggtc cccgatcagg gcaagggagc
     1501 ggcgctcgcg caagagatct tccgcgacgg gatgggcgcg tgcgggggaa cgagaaggag
     1561 ctatccgtct atgaaagccg cgtatcccgc cggcgtccgg cgctcatccc cggcgaacac
     1621 cggccgccgg tggggggcgt tgttcctggc gatgtttctt tccgcgaatg tccatgccgc
     1681 caagccggaa aagattttcc ctccctccgg gaaaccgccg gcgcggtttt ttgtcggag
//
LOCUS       DAOOBX010000209         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_75190_length_1739_cov_3.127078, whole genome shotgun sequence.
ACCESSION   DAOOBX010000209 DAOOBX010000000
VERSION     DAOOBX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75190_length_1739_cov_3.127078"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PLY30_03510"
     CDS             complement(<1..124)
                     /locus_tag="PLY30_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPW65207.1"
                     /translation="MLNKLFKLSERNTDVKTEAVAGLTTFLAMAYIIFVNPSILA"
     gene            complement(173..328)
                     /locus_tag="PLY30_03515"
     CDS             complement(173..328)
                     /locus_tag="PLY30_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65208.1"
                     /translation="MANGDDMGKALPPGGQTHLVFAGEAIIMRANIAHSVKAVQKFKM
                     LTIMIRP"
     gene            446..>1739
                     /locus_tag="PLY30_03520"
     CDS             446..>1739
                     /locus_tag="PLY30_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPW65209.1"
                     /translation="MTKEQSGSPKPLKKSRSLDTSNVHFKTGVAFFLMALIPILLMLY
                     MVFFHMKPVTGEGYRIDLQLLIFLALCSALVGYLIIRKIASAIATMAKSARDLAEGKA
                     LPAEINVKEQSEEIRNLIKVFSGVNQNLEKQVAELEESRRVIQDLLKKIGAVITSEDR
                     TEGFFDLIVDSMVKAMDAETGALLLRGEEGEEKYFRIVVTCGKEAEAVKRLAAQRASV
                     LLWMSGEKHSAIINRSEQPREEEPDPSELPYRSLLCVPLRYQNRGQGCVMVLNRRNGK
                     PFTQEDAGLLEHVASQIAVAAENSRLMVNAERSYVETVAALAVAVEERDSYTHGHLER
                     VARCAVKIGEAMKMNAKDIQTLRDAAFLHDIGKIAIEDKVLLKKEKLTPEEQVIMRSH
                     AEKGEKIIAPLRTFKDLREIVRHHQEWFDGSGYPDGISG"
BASE COUNT          399 a          463 c          522 g          355 t
ORIGIN      
        1 cggcgaggat cgagggattc acgaagatga tataggccat cgcgaggaag gtcgtgaggc
       61 ccgcgaccgc ttcggttttc acgtccgtgt tccgttcgga caatttaaac agtttattca
      121 gcatcattcc tccgacaggt gaccacatcc aacctacgag gttggatttt ggttaagggc
      181 gaatcatgat agtcagcatc ttgaacttct ggaccgcctt gacgctatgg gcgatgttcg
      241 cccgcatgat aatcgcctct ccggcaaaga ccaggtgagt ctgaccgccg ggcggcaggg
      301 ccttccccat atcatccccg ttggccatag gtcctttttg gtctcagtcg tccaggcctt
      361 aaaaacatca aacgcattat agtatggatc cgcgatttta aaaatcccgt tatcggaacc
      421 gttccttttg aagtacaata gaaccatgac gaaagagcaa tccggatcgc cgaagcccct
      481 gaagaaaagc cggtcgcttg acaccagcaa cgttcacttc aagaccggtg tcgcgttctt
      541 tctgatggcg ttgatcccga ttctcctcat gctctacatg gttttcttcc acatgaagcc
      601 cgtgaccggc gagggctacc ggatcgatct ccagttactg atcttccttg ccctttgttc
      661 ggcccttgtc ggttacctga ttattcgcaa gattgcatcc gcgatcgcga cgatggccaa
      721 gagcgcgcgc gaccttgcgg aaggcaaggc cctcccggca gagataaatg tcaaggagca
      781 gtccgaggag attcgcaatc tcataaaggt cttcagcggg gtgaaccaga acctcgaaaa
      841 acaggtagcg gaactcgagg agtcccgtcg cgtcatccag gacctcctga aaaagatcgg
      901 ggctgttatc acctcagagg accgcacgga agggtttttc gatcttatcg tggacagcat
      961 ggtcaaggcc atggacgctg agacgggcgc gctcttgctg cgaggggaag agggagagga
     1021 aaaatatttc cggatcgtcg tcacctgcgg caaggaggct gaggcggtca agcggcttgc
     1081 ggcccagcga gcgtccgtcc tgctatggat gagcggcgaa aagcattccg cgatcatcaa
     1141 tcggtcggag cagccccgtg aagaggagcc ggacccctcg gagcttcctt accggtcgtt
     1201 gttgtgtgtg ccgcttcgtt accagaaccg ggggcaggga tgcgtgatgg tcctgaaccg
     1261 gagaaacggg aagccgttca cgcaggagga tgccgggttg cttgagcacg tggcaagtca
     1321 gatcgcggtc gcggccgaga attcgcggct tatggtgaac gccgaacgaa gctatgtcga
     1381 aacggttgcg gcgctcgcgg ttgccgtgga ggaaagggat tcctacacgc atggccacct
     1441 ggagcgtgtc gcgcgttgcg cggtcaagat cggcgaggcc atgaaaatga atgcgaaaga
     1501 catccagacg ctgcgggacg cggcattcct tcacgatatc gggaagatcg cgatcgagga
     1561 caaggtcctt ctgaaaaaag agaaactcac tcccgaggaa caggtaatca tgaggagcca
     1621 cgcggaaaaa ggcgagaaga tcatcgcgcc gctccggacg ttcaaggatc tccgcgagat
     1681 cgtccggcat caccaggagt ggttcgacgg gtccgggtat ccggacggca tcagcgggg
//
LOCUS       DAOOBX010000210         1732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_75597_length_1732_cov_3.281455, whole genome shotgun sequence.
ACCESSION   DAOOBX010000210 DAOOBX010000000
VERSION     DAOOBX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75597_length_1732_cov_3.281455"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..526)
                     /locus_tag="PLY30_03525"
     CDS             complement(272..526)
                     /locus_tag="PLY30_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HPW65210.1"
                     /translation="MAKANAAFMKPMNISSELAVIVGKGPMPRSEVVKGLWAYIKKNG
                     LQDSKNKRNINADANLKAVFGGKSVVNMFEMTKLVSKHLS"
     gene            complement(643..1101)
                     /gene="nrdR"
                     /locus_tag="PLY30_03530"
     CDS             complement(643..1101)
                     /gene="nrdR"
                     /locus_tag="PLY30_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPW65211.1"
                     /translation="MRCPFCKQDSDKVVDSRSANEGEVIRRRRECTACGKRFTTYERV
                     EEIPMFIVKKDQRREAYDRQKVLSGIYKACEKRPVPLAVQDAIADDLETVIREKYEKE
                     IPSRVIGEFVMQKLARVDQVAYVRFASVYRDFQDVSAFMKELKFLLAKPK"
     gene            complement(1105..>1732)
                     /gene="glyA"
                     /locus_tag="PLY30_03535"
     CDS             complement(1105..>1732)
                     /gene="glyA"
                     /locus_tag="PLY30_03535"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341171.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPW65212.1"
                     /translation="GHHPSPVPYADIVTTTTHKTLRGPRAGLILCREKYAKAIDSAVF
                     PGIQGGPLMHVIAAKAVAFKEAMSPEFREYSGQVVKNARVLADAMGRRGWRIVSGGTD
                     NHLMLVDVTVKGFNGKQVQEALDKVKITCNKNTIPFDKESPFKGSGVRLGTPAVTTRG
                     MKEREMELIAELITEAMQGQGDEGKLAAVRARVEALTKQFPLYPGLKR"
BASE COUNT          318 a          562 c          447 g          405 t
ORIGIN      
        1 ccaggatgat accgattttt gatcccattg aacttttccc ctctgcgttg acccagcccg
       61 ccgcgcggcc accgtcgact accggtcgaa tgtcagccgg tcaaatgcgg gacatcttta
      121 cttcagactt gaggtattgc ttccctcgaa gaacacccgg aagagagggc ccgccgtttg
      181 atcggacacc gccctctctt ccggacctga cctgctcgcg taacgcgcat cccgtcagat
      241 cacgggacgc gtccggaaaa cggatgaaac cttacgagag gtgcttgctg acgagcttcg
      301 tcatctcgaa catattaacg actgatttgc caccgaacac ggctttaaga ttcgcgtccg
      361 cattgatatt gcgcttgttc ttcgaatcct gaaggccgtt tttcttgata taggcccaca
      421 gtcccttcac aacctcgctg cgcggcatcg gccctttacc gacaattacc gcgagttcgg
      481 aactgatgtt cataggcttc atgaacgctg cgtttgcttt cgccattgtg cttctcctca
      541 tggttaaaag gccgccggac ccctacgact cccgcgaacc tgactgttgg ttatcataca
      601 atagacttag gctctctaca ataagagctt tactataggg aattatttcg gcttcgccaa
      661 gagaaacttc aattctttca tgaaggcgct gacgtcctgg aaatcgcgat agacactcgc
      721 aaaccggacg tacgccacct gatcgaccct ggcgagcttc tgcatcacga attccccgat
      781 cacccgcgac ggaatctcct tctcgtattt ctcgcggatc acggtttcga ggtcgtccgc
      841 gatcgcgtcc tgcaccgcga gcggcaccgg acgcttttcg cacgccttgt agatcccgga
      901 aaggaccttc tgacggtcat aggcctcgcg gcgctggtct tttttcacga tgaacatcgg
      961 gatctcctcg acccgctcat acgtcgtaaa gcgtttgccg caggccgtgc attcgcggcg
     1021 tcggcggatc acttccccct cgttcgccga gcgtgaatca acgaccttgt cggaatcctg
     1081 tttgcagaaa gggcagcgca tccgttacct cttgagcccc gggtaaagcg ggaactgttt
     1141 cgtcagcgcc tccacgcgcg cgcgcaccgc cgccagtttc ccctcgtccc cctggccttg
     1201 catagcctcg gtgatcaact ccgcgatcag ttccatttcg cgttctttca tcccgcgcgt
     1261 cgtcacggcc ggcgtcccga gcctcacgcc cgaacccttg aacggcgatt ccttgtcgaa
     1321 cgggatcgtg ttcttgttgc aggtgatctt caccttgtcg agcgcctcct ggacctgctt
     1381 gccgttgaaa cctttcacgg tcacgtccac gagcatgagg tgattgtccg tgccgcccga
     1441 aacgatgcgc catccgcggc ggcccatcgc gtcggcaagc accctcgcgt tcttcacgac
     1501 ttggccggaa tactccctga attcggggct catcgcctcc ttgaacgcga ccgctttcgc
     1561 cgcgatcaca tgcatgaggg gtccgccctg gatgcccggg aaaaccgccg agtcgatcgc
     1621 cttcgcatat ttttcccggc agaggatcag acccgcgcgc ggcccgcgaa gggtcttatg
     1681 ggtcgtcgtt gtcacgatgt ccgcgtacgg gaccgggctc ggatggtggc cg
//
LOCUS       DAOOBX010000211         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_75750_length_1729_cov_6.894863, whole genome shotgun sequence.
ACCESSION   DAOOBX010000211 DAOOBX010000000
VERSION     DAOOBX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75750_length_1729_cov_6.894863"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..955)
                     /locus_tag="PLY30_03540"
     CDS             complement(179..955)
                     /locus_tag="PLY30_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65213.1"
                     /translation="MRKVVLAIFLLLVILAGVSAPSAQAAMPTFDAINAALQELQNSI
                     LNSSFAQDLMLAAERLNQLKTQTLELFRFHAGFDEILNFVKGDPVRDFIDSMRTKIRD
                     AFSDAIFTTPQIEILQTGGSAEDLRTSLEAITGAIPEGNARPYIPFEEMQVVEAFQLA
                     SQIREGGKDLRDAARQIQAQAETASPKGAVRLQAQGAAELMVLGQQTQESIAKLLELE
                     ATQIEQVSRREKDAERERLRFIKDTSDYLDGILDASGGVR"
     gene            complement(948..>1729)
                     /locus_tag="PLY30_03545"
     CDS             complement(948..>1729)
                     /locus_tag="PLY30_03545"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW65214.1"
                     /translation="PLLGDIEKGLREFSDGDEEDKRKAYQFSKELSLFTQGEYGKILN
                     RKGTFDFNARFTVFDLRKISRYPELQEILLFIIPFALKRKFENLGIKKILVLDECWQL
                     LKESQGTELVEVFYRTARKLNAGVLSISQNPEDFLESKISGVMVNNSPIKYILRLKKG
                     HEKLASFGLNENEILAARELEVRPGHYSEVFIKFDDHGVIAKVEPNPLEYWIATTDPA
                     DLAQETELRVAAPLKTNLEIFETLARKFPGGASKPREAANA"
BASE COUNT          366 a          497 c          478 g          388 t
ORIGIN      
        1 ataaaaaggc ctttgtaatc cgagcggtct ttcatcacga gaaagttctc gtgcagcacc
       61 gccacgacga acatgagcgg aaagatcacg gccgcggcag aggccgtcac cccgtaggcg
      121 gatgcgaaaa gatccgggat ctgaaggacg ttcgtgaaaa gaaatccctg gttcatcatc
      181 accgcacccc gcctgacgcg tccaggatgc cgtccagata atcgctcgtg tccttgataa
      241 agcggagacg ctcccgctcc gcgtcctttt cccggcgcga gacctgctcg atctgagtag
      301 cctcaagctc gagaagtttc gcgatcgatt cctgggtttg ctgtccgagg accatcagtt
      361 ccgcagcccc ctgcgcctga agtctgactg cgcctttggg actcgccgtc tccgcctggg
      421 cctggatctg ccgggccgcg tcccggaggt cttttcctcc ctcgcggatc tgacttgcca
      481 gttgaaacgc ctcgaccact tgcatctcct cgaacgggat atatggccgc gcgttcccct
      541 caggaattgc gcccgttata gcctcaagcg aggtccggag gtcttcggcg cttccccccg
      601 tctgaaggat ctcgatctga ggcgtcgtga agatcgcgtc cgagaaagca tcccggatct
      661 ttgtcctcat ggaatcaatg aagtcgcgga ccggatctcc tttgacgaaa ttcaggatct
      721 catcgaagcc cgcgtgaaac cggaaaagct caagggtctg ggttttcagc tggttcaagc
      781 gctcagcggc aagcatgagg tcctgcgcaa aactgctgtt caggatggag ttctggagct
      841 cctgaagagc ggcgttaatg gcgtcaaagg tcggcatcgc ggcctgggcg gaaggcgcag
      901 aaacacctgc gaggatcacc agcagaagga aaatagccag aacgacttta cgcattcgcg
      961 gcctcccggg gttttgacgc gcctccggga aacttccggg ccagcgtttc aaagatctcc
     1021 aggttcgttt taagaggcgc cgcaacacga agctccgttt cctgggccag atccgcgggg
     1081 tctgtcgtgg cgatccagta ttccagcggg ttcggttcca ctttcgcgat caccccgtga
     1141 tcgtcgaact tgataaagac ctccgaataa tgccccggcc ggacttccag ttcacgggcg
     1201 gcaaggatct cgttctcgtt aagaccgaag gaggcgagct tttcgtgccc tttcttcagg
     1261 cgcaggatgt atttaatggg agagttattg accatcacgc ccgagatctt tgactccagg
     1321 aaatcctccg ggttctggct gatcgaaaga actccggcgt tcagtttccg ggccgttctg
     1381 tagaacactt cgacaagttc cgtcccttgg gattccttga ggagctgcca gcattcatcg
     1441 agcacgagta tctttttgat cccgagattc tcgaacttcc gtttcagggc gaagggaatg
     1501 ataaaaagca ggatctcctg aagctcggga taacgggaga tctttcggag atcgaaaacc
     1561 gtgaaacgcg cgttaaagtc gaacgtgccc ttccggttca ggatctttcc gtactcgccc
     1621 tgggtaaaaa gagagagctc ttttgagaac tggtaggcct tccgcttgtc ctcttcgtct
     1681 ccatccgaaa attcccggag acctttctcg atatctccca aaagcggag
//
LOCUS       DAOOBX010000212         1715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_76626_length_1715_cov_2.396386, whole genome shotgun sequence.
ACCESSION   DAOOBX010000212 DAOOBX010000000
VERSION     DAOOBX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76626_length_1715_cov_2.396386"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PLY30_03550"
     CDS             <1..1113
                     /locus_tag="PLY30_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPW65215.1"
                     /translation="GTPRYDLGIQQKEDAQHRLIDVWATGEASASLGFEAARIFDEFD
                     SLEKQKDAMFLKEGIKGPRAQMKVFAEPRKKALAYVAETGKPEARRDPKVLEAYNNDF
                     LVQYVVKESLADVFCPACKLWNTGYGATMMREAVSLMGGYGITEDCPGFLTQKWSDAQ
                     LEATYEGPECVQRRQMSLTMTNDVFLAQFRNWIKDLRQLDATKPGMGAGTLAGAMELW
                     LWSVEYLRNAKDANGKTLYGGQRQGVTFPMADALAWMLGSRQLILDTLELEKKGPENP
                     TLAEGLPGFVQFYSDLSCVQAARAAGEVAKICAELVCGYHAKGATCALPDAALKTFSE
                     LREKLECSLAGARLAKDRAGEAASQVMIPEALDYPL"
     gene            1237..>1715
                     /locus_tag="PLY30_03555"
     CDS             1237..>1715
                     /locus_tag="PLY30_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HPW65216.1"
                     /translation="MTADRQKMDVDIVCTGFGPASAGFLTTLNKAMAADPTAEHFQSK
                     VMPGMPLQVICYERADDIGFGVSGIVTEAKGIKASFTPEELAQVPMAHPVKHEEVYYL
                     LDPVGASRRSWLLKLVDAVLRVIGVILHWFRDHSFRLPFIPPFMAKHGGWVFSMGQFT
                     "
BASE COUNT          333 a          500 c          540 g          342 t
ORIGIN      
        1 ggaaccccgc gctacgacct cggcatccag cagaaagagg acgcgcagca ccgtctgatc
       61 gacgtgtggg cgaccggcga agccagcgcc tcgctcgggt tcgaagcggc ccgcattttc
      121 gacgagttcg attcgcttga gaagcaaaag gacgcgatgt tcctgaaaga aggcatcaag
      181 gggccgcgcg cgcagatgaa ggtattcgcg gaaccgcgca agaaggcgct cgcttatgtc
      241 gcggagacgg gaaaacccga agcccggcgc gatccgaagg tccttgaagc ctacaacaat
      301 gattttctcg tgcagtatgt cgtgaaagag tcacttgccg acgtgttctg tccggcctgc
      361 aaactctgga acacgggcta tggcgcgacg atgatgcgcg aggccgtttc cctcatgggc
      421 ggctacggga tcacggaaga ctgccccggg tttctcacgc agaaatggtc ggatgcgcaa
      481 ctcgaggcca cctacgaagg ccccgaatgc gttcagcgcc gtcagatgtc gctcacgatg
      541 acaaacgacg tttttcttgc ccagttcagg aactggatca aggatctccg ccagcttgat
      601 gcgacgaagc ccggcatggg cgccgggacg ctcgcgggcg cgatggagct ttggctttgg
      661 tcggtcgaat atctccggaa cgcaaaggac gcgaacggga aaacgctcta cggcggtcag
      721 cgccagggcg tgacgttccc gatggccgac gcgctcgcgt ggatgctcgg ttcccgtcag
      781 ctcatcctcg acacgcttga gcttgagaag aagggtcccg agaatccgac gctcgcggaa
      841 ggccttccgg ggttcgttca gttctatagc gacctctcct gcgttcaggc cgcccgcgcg
      901 gccggtgaag tcgcgaagat ttgcgccgaa ctcgtgtgcg ggtatcatgc gaagggcgca
      961 acctgcgctc ttccggatgc cgcgctcaag acgttctcgg agctccgtga gaagctggaa
     1021 tgctcgcttg ccggcgcgcg cctcgcgaag gaccgcgcgg gcgaagccgc ctcacaggtg
     1081 atgatcccgg aagcgttgga ttatcctctt taagatcagt acggggtatg cggtgcgcgg
     1141 agtgaagggc cttgtgtttc tttctccgcg ctccgtgccc cgtacggaca aagccctttt
     1201 acgggaaatt ttcgaccaca ggatggagat tgaaagatga ccgctgaccg ccagaagatg
     1261 gatgtcgata tcgtttgcac cggattcggg cccgcgtccg ccgggttcct cacgacgctc
     1321 aacaaagcca tggcggctga tcccacggct gagcactttc aaagcaaggt catgccgggc
     1381 atgccgctcc aggtgatttg ctacgagcgg gcggacgaca ttggttttgg cgtttcgggg
     1441 atcgtgaccg aagcgaaagg gatcaaggcc tcgttcacgc cggaagagct tgcgcaggtc
     1501 ccgatggcgc atcccgtgaa gcatgaagag gtctattacc tcctcgatcc cgtcggcgca
     1561 agccgccgct cctggctttt gaagctcgtt gacgcggtcc ttcgtgtcat cggtgttatt
     1621 ctccactggt tcagggatca ctcgttccgg cttcccttta tcccgccttt tatggcgaaa
     1681 cacggcggct gggtgttctc gatgggacag ttcac
//
LOCUS       DAOOBX010000213         1706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_77213_length_1706_cov_2.640218, whole genome shotgun sequence.
ACCESSION   DAOOBX010000213 DAOOBX010000000
VERSION     DAOOBX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77213_length_1706_cov_2.640218"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1300)
                     /gene="ychF"
                     /locus_tag="PLY30_03560"
     CDS             complement(206..1300)
                     /gene="ychF"
                     /locus_tag="PLY30_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPW65217.1"
                     /translation="MGFRCGIVGLPNVGKSTIFNALAKAHAAASNYPFCTIEPNVGMV
                     PVPDQRLNELSKLYNPQKTTPTMVEFYDIAGLVAGASKGEGLGNQFLGHIKEVEAILH
                     VVRCFEDPNIVHVGSTVDPVRDIEVIDTELCLKDLDTINARYNKTEKLAKTGDKDAKE
                     KLPIYERVKKTLEDGIPLRRAEFSDKEKELIKDLAFLTLKTVLYGANVAESDLPSGGK
                     HVDAVRKKAAEEGAEVVVISGKVESELIDLPEEDQKVFLKELGLTEPGLYSLIRAGYR
                     ALNLITYFTAGEKEVRAWTVRNGAKAPEAAGVIHSDFERGFIRAEVMRCEDLLKFKTS
                     VAVKERGLLRIEGKEYVVQDGDTMLFRFSV"
     gene            1476..>1706
                     /locus_tag="PLY30_03565"
     CDS             1476..>1706
                     /locus_tag="PLY30_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65218.1"
                     /translation="MRHRKILRWMSLVTLALTAGYQTYSAAEWQRPLQPSLSGGELVC
                     LAAHPREGNGFFVANSFQLFEGADGSGWKPVWS"
BASE COUNT          374 a          481 c          459 g          392 t
ORIGIN      
        1 aacgaaaact gacggagcgg ctcgccgctc atgaacttgc gaccgcggat gtcctgtgcc
       61 ggttcatcaa gttgattgaa gaaccgctca aatcagacat ccctcagaaa tttccgatcg
      121 gatttctgcc ttgagttcgc acaaaccgat tccccatgga atttgaggag gatatcaggg
      181 ccaagcggac actataaccg caggatcaga ccgaaaaacg gaagagcatc gtgtcgccgt
      241 cctgcacgac atattctttg ccttcaatgc ggaggagtcc cctttctttc acggccacgg
      301 aggttttgaa cttgaggagg tcttcacagc gcatgacctc ggcgcggatg aacccgcgct
      361 cgaaatcaga gtggatgaca ccggccgctt cgggcgcctt cgcgccattc cggacggtcc
      421 aggcacggac ctctttttcg ccggccgtaa aataggtgat gaggttcagg gccctgtagc
      481 ccgcccggat cagggaataa agccccggct ccgtgagccc gagctccttg agaaacacct
      541 tctgatcctc ttccgggagg tcgatcagct cggactcgac cttgccggag atcacgacca
      601 cttcagcgcc ttcttcggcc gcttttttcc ggaccgcatc gacatgtttc ccgcccgaag
      661 gcaaatccga ttcagccacg ttcgcgccgt aaagcacggt tttgagagtc aggaacgcga
      721 gatctttgat cagttctttc tctttgtcgg aaaactccgc gcggcgaaga gggattccgt
      781 cttcaagcgt tttcttgacg cgctcataga tcggaagctt ttccttcgcg tccttgtcgc
      841 ccgtcttcgc gagtttctct gtcttgttgt aacgcgcgtt gatcgtgtcg aggtctttca
      901 ggcagagctc ggtgtcaatc acctcgatat cgcggacggg gtccaccgtg gaacccacat
      961 gaacgatgtt cgggtcctca aaacaacgga cgacgtgaag gatcgcctca acctccttga
     1021 tatgcccgag gaactggttg ccgagacctt cgcctttgga agcgccggcc acgaggcccg
     1081 cgatgtcata gaactcgacc atcgtcggcg tggttttctg ggggttataa agtttggaga
     1141 gttcgttcag gcgctggtcc ggcaccggta ccatgccgac attcggctcg atcgtgcaaa
     1201 aaggatagtt cgacgccgcg gcgtgcgcct tggcgagagc gttgaagatc gtggatttgc
     1261 cgacgttggg gagtccaaca ataccgcatc gaaagcccat aatagaggga gactatagca
     1321 gaattagctc cgtcacgcta ttctcaaaaa acggggatag ccgtttcccc ccttaataaa
     1381 cagcaaccgg tgctatgtgg gaaactcaaa agcttcttga caatattata ttatatgcat
     1441 atattctatg ataaatacac aaacaggagg atgtcatgcg tcaccgaaaa atcctgcgct
     1501 ggatgtccct ggtcaccctc gccctgacag cgggctatca gacttattcc gccgcggaat
     1561 ggcaacggcc tttacaaccc tccctttcag gaggggagct cgtctgcctc gccgcccacc
     1621 cgcgagaagg caacggattc tttgtcgcga attcctttca gctttttgag ggggcggatg
     1681 gttcaggctg gaaaccggtc tggtca
//
LOCUS       DAOOBX010000214         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_77337_length_1704_cov_3.280170, whole genome shotgun sequence.
ACCESSION   DAOOBX010000214 DAOOBX010000000
VERSION     DAOOBX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77337_length_1704_cov_3.280170"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="PLY30_03570"
     CDS             complement(<1..1583)
                     /locus_tag="PLY30_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65219.1"
                     /translation="MLETENRAGDVLAMARKSLEGDGFAALTVSGLIKTWMKSEPAES
                     PLYQSSAWLNEQMAPWSEKLDAVKTGRNDQLFIEAVLAAALVLERDRDPRAAENRIRN
                     VYEQFAAKNISSEVSAMLALAFVFADGASIALRQQYTLRYFRQFQRENYSPVESALAT
                     IHLLKTRIRAFEHGHRGIFGRFLNKRVSERIAALLTAAFYAQGREQFLTNDIFTGYTA
                     LLKEGYEDSQAAALTLIRTILGAWAMMKEKENIRALADRLTGLGDEFVRSEVREERPV
                     RIMTRMWKKAPESAPGVFARSESRAWAKKPAELGKYTVVSRQLEAGLSRDILDRLIQK
                     FGETIAGLVAATTVTGGLGALMHDLFPSWQKNFGRPKAKAYDAFTVNVIYDEIKGQRF
                     AKDVPEEVKEGKRTLGDYLRDVLNEDPGLRLETFMEPGSEYRSGAEEWISRAEGIIAD
                     IDGRISGSGDANARAGLEAQKKDWTLYRKRLQQAREASDREIRIKVYPTETSVGGMPN
                     FYVEAFYIDEYGAKVQIFD"
BASE COUNT          349 a          536 c          426 g          393 t
ORIGIN      
        1 tcgtcaaaaa tctgtacttt cgcaccgtat tcatcgatgt aaaaggcctc cacgtaaaag
       61 ttcggcatcc ccccgactga tgtttccgtg gggtaaacct tgatccggat ctcgcgatcg
      121 gaagcttccc gcgcctgctg aagccttttc cggtaaagcg tccaatcctt tttttgagct
      181 tccagtcccg cgcgcgcgtt cgcgtccccc gatccggaga tccggccgtc tatatccgcg
      241 atgatccctt ccgcgcgtga aatccattct tccgcgccgg agcgatattc acttccgggt
      301 tccatgaaag tctccagacg aagaccgggg tcttcgttga gaacatcccg caaataatct
      361 cccagagtcc ttttgccctc tttaacttct tccggaacgt ccttggcaaa acgctgtccc
      421 ttaatctcat catagatcac gttcacggta aacgcgtcat aggctttcgc cttgggcctc
      481 ccgaagtttt tttgccagga agggaaaaga tcgtgcatca aggcgccaag accgccggtc
      541 actgtcgttg ccgccacaag acccgcgatc gtttccccaa acttttgaat aagcctgtcg
      601 agaatatctc ttgaaagccc cgcttcaagc tgacgcgata caaccgtata cttccccagt
      661 tccgcgggct ttttcgccca ggcgcgtgat tcggagcggg caaaaacccc gggagcagat
      721 tccggcgctt ttttccacat acgcgtcatt atccgcacag gacgctcttc ccggacttcg
      781 ctccggacaa attcgtcccc gagacccgtc agtcgatccg caagcgccct gatattctct
      841 ttctccttca tcatggccca agcgcccagg atggtccgga taagcgtcag ggccgcggcc
      901 tgcgaatctt catagccttc tttcaggagc gccgtataac cggtaaaaat atcatttgtc
      961 aggaattgtt cccgtccctg ggcgtagaaa gcggccgtca gcagcgccgc gatccgttcg
     1021 ctgacgcgtt tgttcaggaa gcgaccaaaa atcccgcggt gcccgtgttc aaaagcgcgg
     1081 atccgggtct ttaaaaggtg gatcgtcgcg agcgccgatt cgaccggcga gtaattctct
     1141 ctctggaatt gacggaaata ccggagcgta tactgctgcc ggagagcgat cgacgcgcca
     1201 tccgcgaaaa cgaacgcaag ggcgagcatg gccgaaactt cggaggagat attcttcgcg
     1261 gcaaattgtt catagacatt ccggatccgg ttttctgcgg cacgcgggtc gcgatcgcgt
     1321 tcgagaacaa gcgccgccgc caaaacagcc tcgatgaaca actggtcgtt cctcccggtc
     1381 ttgacggcat ccagtttttc ggaccacggc gccatctgtt cgttcagcca cgcggaggac
     1441 tgatagagag ggctttctgc aggttccgac ttcatccacg ttttgattag cccggaaacc
     1501 gtcaacgcgg caaatccgtc cccttccagg gactttcgcg ccatcgcaag gacgtcgccc
     1561 gcgcggttct cggtctcaag catcgaccgt atcctcagaa catcaggcgt ttcacggacc
     1621 tcgctgcgcg catcctgtcc ggtggctctg aaccatcggc tcagcctatc catatcccga
     1681 tcccgcatcg tctgaccttt ccgg
//
LOCUS       DAOOBX010000215         1699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_77698_length_1699_cov_2.658151, whole genome shotgun sequence.
ACCESSION   DAOOBX010000215 DAOOBX010000000
VERSION     DAOOBX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77698_length_1699_cov_2.658151"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="PLY30_03575"
     CDS             <1..1446
                     /locus_tag="PLY30_03575"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HPW65220.1"
                     /translation="QHWRKPLRWALDWLRDELARVYEKEAAKYLKEPWDARDDYIRVI
                     LDRSEKAIREFFYKHGLRELSRPEVTSCLKLLEMQRYLMLMYTSCGWFFSEISGIETV
                     QILQYAARAVELGEEVSGLKFEREFLDHLSLAKSNMELFKDGQGVYEKLVKPSRAGLH
                     HIVSYYAICSIFEDYYAKSDVVSMYANNLHILHQRKEFFGTITMNFGRVRVVSRVTLE
                     ERDLVFVVIQIGFYDFRCSVMPYKDEASFDALERDLFDALYQLPILDLSKKIDACFGE
                     AYYALKDLRLGDRVNIINRLTHETIEKVSNFYENIYEENLRMNEIYRSIKVPIPVEFR
                     YAAEHTLGKRLDEAVREWGSQGFPAKRTLPVYRVIDSAKALDIQVHKSPIAKYLSDEL
                     ASLVRGFVAEPGAAAIERSLSILKLSRKIDVFLDKRVAQDDLFSLVRSWKSIVQAVPP
                     VIREQENHFLQLMEELDLSTDRVRKMFGHAA"
BASE COUNT          361 a          466 c          484 g          388 t
ORIGIN      
        1 cagcactggc gcaagccgct tcgctgggcg cttgactggc tccgcgacga acttgcccgc
       61 gtgtacgaaa aagaggccgc caagtacctg aaggaaccct gggacgcccg cgacgattac
      121 atccgcgtca tcctggaccg gagtgaaaag gcgatccggg agttctttta caagcacggg
      181 ctgcgcgagc tttccaggcc ggaggtaaca tcctgcctca aactgctcga gatgcagcgt
      241 tatctcatgc tgatgtacac aagctgcggc tggttctttt ccgagatttc gggtatcgag
      301 accgtgcaga tcctccagta tgccgcgcgc gcggtcgagc tcggtgagga ggtaagcggt
      361 ctcaagttcg agagggagtt cctcgaccac ctgtcgctcg caaagagcaa tatggagctt
      421 ttcaaggacg ggcagggtgt ctatgaaaag cttgtgaagc cctcgagggc cggccttcac
      481 catatcgtga gctattacgc gatctgctcg atctttgagg attactacgc caagagcgat
      541 gtggttagca tgtacgccaa caatctccac atcctccatc agcgcaagga gttcttcggg
      601 acgatcacga tgaatttcgg ccgtgtccgc gtggtatcgc gtgtcacgct ggaggagcgc
      661 gacctcgtct tcgtcgtgat ccagatcggt ttttatgatt tccggtgctc ggtcatgccg
      721 tacaaggacg aggcgtcgtt tgacgcgctg gagcgggacc tctttgacgc gctctaccag
      781 ctgccgatcc tcgatctttc gaagaagata gacgcctgtt ttggggaggc ctattacgcg
      841 ctgaaggatc tccgcctcgg cgaccgcgtg aatatcatca accgtctcac gcacgagacg
      901 atcgaaaagg tcagcaattt ctacgagaat atctatgagg aaaacctccg catgaacgag
      961 atctaccggt cgatcaaggt tccgattccg gtggagttcc gctacgcggc cgaacacacc
     1021 ctcggtaagc ggctggacga agcggttcgc gagtggggga gccagggatt tccggcgaag
     1081 cgcacgcttc ccgtctaccg ggtcattgat tcggcgaagg cgctcgatat ccaggtccac
     1141 aagtcgccga tcgcgaagta cctgagcgac gaattggcct cgcttgtccg gggttttgtc
     1201 gcggaacccg gagccgccgc gatcgagcgg tcgctttcca tcctcaaact atccaggaag
     1261 atcgatgttt ttctcgacaa gcgggtcgcg caggatgatc ttttttccct cgtccgttcc
     1321 tggaaatcga tcgtgcaggc ggtaccgccc gtgatccgtg agcaagagaa tcattttctc
     1381 cagttgatgg aagagctgga tctttccacc gaccgcgtcc ggaaaatgtt tggccacgcg
     1441 gcttgagagg gcgtgtttcg ttgatctttt ttcgggaagg atcagcgcac gaaatatggg
     1501 aaattttttc atgattgctc ttgagtaaga gcaggaaagt ttttgtgaat ttaacccatc
     1561 atttctgatc gaggtgaaaa gaccaaatcc gaaaaaaata cgctgacctg attagcctgc
     1621 cccgttcgtt ccgaatttct cgcctagcct tccaatgcct ccacccactc tacccccggg
     1681 agctagaccc cgggggtgg
//
LOCUS       DAOOBX010000216         1696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_77887_length_1696_cov_3.641682, whole genome shotgun sequence.
ACCESSION   DAOOBX010000216 DAOOBX010000000
VERSION     DAOOBX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77887_length_1696_cov_3.641682"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(497..943)
                     /locus_tag="PLY30_03580"
     CDS             complement(497..943)
                     /locus_tag="PLY30_03580"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPW65221.1"
                     /translation="MLKRFLVFFAAALAVLLVLCWIYLPSFSRYHELKQEEERLSRRI
                     GEIDSQIKLLTDEKYLLQNDVAYLEKVIREELGFVKPGEVIYDLVTRKNSTGPVAVAE
                     DPKPATTTVAVTAPAPDTRTPTATSKSGSSRKVRAPSSRNTSAKRT"
     gene            complement(943..>1696)
                     /gene="eno"
                     /locus_tag="PLY30_03585"
     CDS             complement(943..>1696)
                     /gene="eno"
                     /locus_tag="PLY30_03585"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839066.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPW65222.1"
                     /translation="VFHNLKKILHAKKLSTAVGDEGGFAPDLGSNDEALEVIMEAIAK
                     AGYKAGRDVKIALDPASSSFYGQDKFGGNRNPGKYVLCAEARKVKSAEDMVEFYADLT
                     KKYPIFSIEDGLAENDWDGWKKLTDRLGKKTQLVGDDLFVTNTQRLAMGIERKTANSI
                     LIKVNQIGTLTETIEAVQLAHKNGYTAVMSHRSGETEDTTIADLAVALSCGQIKTGST
                     SRTDRICKYNRLLRIEEALGAKAVYAGLKLGK"
BASE COUNT          355 a          491 c          463 g          387 t
ORIGIN      
        1 cggacggcgc aaccattatc atcgcgtaag aacggtccgc ccggtatcct tcggcgcgta
       61 gagataaacc gggtatttgt agtccgcaat cgcaagaagc cccgaaggaa acggatcgct
      121 ggcctcgcct gcagacacat tcgcggacac ggcctgcccc tgcccgggag cattcgcttg
      181 gtccggggtg gaaaattcca ccagttcccg cgaaaatgcg ggtaccggat tcaaaacaca
      241 gcaaacgata aaaaacggga caaaaaatct cttttgcata cgccacataa gaatagcctt
      301 tgatctcgcg gaggtttagg tgatgtaagt gcctgaaacc aaagaagtta ttattatatc
      361 gattcaattt atgatgtcaa aggcagagta ccctgaaatc ccgggtgtat tgagacccgg
      421 gatgcttttt cgcaacctgt tgaccggaca caagttgcaa tggccacacg caacacgcaa
      481 gcactccgat agaggctcag gtccgtttgg cggatgtgtt cctggatgac ggggcccgga
      541 cttttcggga acttccggat ttcgaagtcg cggtcggcgt cctggtatcg ggagcggggg
      601 cggttacagc aacggttgtc gtcgcgggtt tgggatcttc cgcgaccgcg acggggccag
      661 ttgagttttt ccgggtcacg agatcgtaga tgacctcacc cggtttcacg aaaccgagct
      721 cctcgcggat gaccttttcc agatacgcga catcgttctg gagaaggtat ttttcatccg
      781 tgagcaactt gatctgggaa tcgatttccc cgatccgtct ggaaagcctc tcctcttcct
      841 gcttcagttc gtggtaccgc gaaaaagacg gcaggtaaat ccagcacagg accagcagga
      901 ccgccagggc cgccgcaaaa aaaacgagga aacgcttcag cattacttcc cgagcttcag
      961 acccgcgtag acagccttcg cgccgagagc ctcctcgata cgcagcagac ggttatactt
     1021 gcaaattctg tccgtgcggg aggtcgaccc ggtcttgatt tgaccgcagc ttaacgcgac
     1081 cgcgaggtcc gcgatcgtcg tgtcctcggt ctcgccgctc cggtggctca taacggccgt
     1141 gtagccgttc ttgtgcgcca gctggacggc ttcgatcgtt tccgtcaggg tcccgatctg
     1201 gttcaccttg atcaggatgg agtttgccgt tttccgctcg atccccatcg cgaggcgctg
     1261 cgtgttcgtc acgaaaagat catcgcccac gagctgggtc ttcttgccga ggcggtcggt
     1321 cagtttcttc cagccgtccc agtcgttttc ggcaagaccg tcctcgatcg agaagatcgg
     1381 atatttcttc gtaagatccg catagaactc gaccatgtct tcggccgact tcacctttct
     1441 ggcttcggcg caaagaacgt acttgccggg attcctgttc ccgccgaact tgtcctggcc
     1501 atagaaactc gagctcgccg gatcgagggc gatcttgacg tccctgccgg ccttgtagcc
     1561 cgctttcgcg atcgcttcca taatcacttc cagggcctcg tcgttcgacc cgaggtccgg
     1621 agcgaaaccg ccctcgtcgc caaccgccgt cgaaagtttc ttcgcgtgaa gaatcttctt
     1681 caggttatgg aacact
//
LOCUS       DAOOBX010000217         1692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_78127_length_1692_cov_4.365913, whole genome shotgun sequence.
ACCESSION   DAOOBX010000217 DAOOBX010000000
VERSION     DAOOBX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78127_length_1692_cov_4.365913"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PLY30_03590"
     CDS             complement(<1..647)
                     /locus_tag="PLY30_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65223.1"
                     /translation="MVRRLARLVLVPLSFFSVILLHPFPSRAVDTPSPALEALLKQVE
                     NRNFRWIAIRADVILFFVAPGTTRQAMCGGELVYQRLDERMLLSCHDQEKNPVFIFRT
                     MDRRFDLYVPSQNTLYHGSVFDLEDSPEVESHLKPLDLFRALKPGMFDPKRTEVDNSS
                     GISVSLNVFAENDRQRFLSRKAYLTPAGDVIGELFFDPEGQTMPEIQRYDFREISS"
     gene            complement(657..1247)
                     /locus_tag="PLY30_03595"
     CDS             complement(657..1247)
                     /locus_tag="PLY30_03595"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPW65224.1"
                     /translation="MLRKTVYPMIEGFVGKLVDQLNTLGITPNQLTLAGCAVNLLAGV
                     IYAKGYLIFGGLVLLIAGLGDMLDGPLARKTGKASPFGAFLDSTVDRYSDFFIFGGLA
                     VYFAREGEVFWFLTSLGILLGAMAVSYTKARAENFIKDCGVGFFGRAERIVLLAFGTL
                     FFPVLKFVLLALLIGTHVTAIQRILHTKKALEGGRS"
     gene            complement(1260..>1692)
                     /locus_tag="PLY30_03600"
     CDS             complement(1260..>1692)
                     /locus_tag="PLY30_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPW65225.1"
                     /translation="NTGGNTDFLNMLNRARLESKKISKTSAVQSQLDTPLEADNIHIG
                     PSDYVPWQNDNKVCYLRIEGRGFAGFPVSLECRLSVEDSPNSAGCSIDAIRACKIARD
                     RKIGGPLISMSAYTMKHPPKQIADYEARELVRKFVQGTCPR"
BASE COUNT          393 a          447 c          524 g          328 t
ORIGIN      
        1 gaggagattt ccctgaaatc atatcgctgg atctccggca tggtttggcc ctcaggatca
       61 aagaacagct ccccgataac gtcccccgcg ggagtcagat aagccttgcg cgaaaggaac
      121 ctctgcctgt cgttttcggc aaaaacattc agactgaccg aaatcccgga ggaattgtcg
      181 acttcagtgc gcttcggatc gaacattccc ggcttcagcg cgcggaaaag gtcgagtggt
      241 ttcaggtggc tttcgacttc cggcgagtct tcgaggtcaa aaacagaacc gtgataaagg
      301 gtgttttggg atgggacgta gagatcgaac cggcggtcca tggtgcggaa aatgaacacc
      361 gggttttttt cctggtcatg gcaggaaagc aacatccgct cgtcaagacg ctgatacaca
      421 agttcgccgc cgcacatggc ctgccgtgtc gttccggggg cgacgaagaa aagaatcaca
      481 tcggcgcgaa tggcgatcca gcgaaaattg cggttctcga cctgcttcag gagagcttca
      541 agggctggcg acggcgtatc tactgcccgg gacggaaaag gatgaaggag gatgacggaa
      601 aagaaagaaa gcgggacgag aaccagccgt gcgagccgtc ggaccatcag ccgggactag
      661 gatcgccctc cttcaagggc ctttttggtg tgaaggatcc tctggatcgc ggtcacatgg
      721 gtgccgatga gcagggccag gagcacgaat ttcaggaccg ggaagaagag ggtcccgaac
      781 gcgaggagca cgatgcgctc ggcccgtcca aaaaaaccga caccgcaatc cttgataaaa
      841 ttctcggctc tcgccttcgt gtagctgacc gccatggcgc ccagcaggat tccgagcgat
      901 gtcaggaacc agaagacctc tccttcgcgc gcgaaataga ctgcaagacc tccaaagatg
      961 aagaagtccg aatagcggtc gaccgtggaa tcaagaaagg ccccgaaagg actggccttg
     1021 ccggtcttcc tggcaagagg accgtccagc atatcgccga gtccggcgat taacaggacc
     1081 agtccgccaa agatcaggta tcctttggca tagatgaccc cggcgagcag attgaccgcg
     1141 catcccgcga gcgtcaactg gttgggggtg atcccgagcg tgttgagttg gtcgacgagc
     1201 ttgccgacaa acccttcaat catcggataa acggtttttc tgagcatgga gaggtcctcc
     1261 tagcgagggc aggtgccctg cacgaacttc cggaccagtt cgcgcgcttc gtagtcggcg
     1321 atctgtttcg gcggatgctt catggtgtaa gccgacatcg aaatgagcgg tccgccaatc
     1381 ttgcggtcgc gggcgatctt gcaggcgcgg atcgcatcaa tggaacaacc ggcgctattc
     1441 ggagagtctt cgaccgaaag gcggcactcg agcgacacgg ggaaaccggc aaagccgcgg
     1501 ccttcgatgc ggaggtagca gaccttgttg tcgttctgcc acggaacgta atcggaagga
     1561 ccgatatgga tattatcggc ttcgagaggt gtgtcgagct ggctctggac cgcggaggtc
     1621 ttcgaaatct tcttcgattc gaggcgggcg cggttgagca tgttgaggaa gtccgtgttg
     1681 ccgcccgtgt tg
//
LOCUS       DAOOBX010000218         1685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_78626_length_1685_cov_2.479141, whole genome shotgun sequence.
ACCESSION   DAOOBX010000218 DAOOBX010000000
VERSION     DAOOBX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78626_length_1685_cov_2.479141"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLY30_03605"
     CDS             complement(<1..762)
                     /locus_tag="PLY30_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPW65226.1"
                     /translation="MTRIALVVFLLFAAVPSAWTLDPDRLSDADIAMQKQVIAKLEPL
                     IRKRDSEKNLAKLTFDELYAPLNEKEIAFVKEFRNISRKDAGVRIPWRGIATGKEDLV
                     ILKGQRVKEKVVVKNPDGTKKTKLQTRVLPPQFLPPDVYIAYEKMMSAMQKDIGKRLY
                     VESGYRSSAFQLYLFIYYLKNHDYSVRETVKFVALPGYSEHGSTKYQALDFINEDGVN
                     GDPNVREFEVLDESKWLHKNAHRFGFVISYPKNGGK"
     gene            complement(759..>1685)
                     /gene="fmt"
                     /locus_tag="PLY30_03610"
     CDS             complement(759..>1685)
                     /gene="fmt"
                     /locus_tag="PLY30_03610"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPW65227.1"
                     /translation="MKIVFFGSSDFSLKPFEACLASGHEVVLVVTTPPKRKGRGLHLE
                     PTAVRAAADKHQLPAGEFPDLREPSVLQKLMALAPDIFVVASYGKIIPPALFTIPRYR
                     LNVHPSLIPLYRGAAPLNWPILNGDAETGVSIIDIAEKMDAGDVYYQTRYPIHPAMNA
                     AELSHELAKLSAEALATVFAKIAKGELMGVPQDESRATVARKLTKEDGLVSFSDPAVT
                     TGRKVRGLAPWPGACFFFQSERVTIVSADPVPCEASEPGMILSIDKSGSVVVATGADA
                     LRIFRVKPEGRNVMPAADFLNGRRLSVGAVLK"
BASE COUNT          314 a          468 c          541 g          362 t
ORIGIN      
        1 cttcccgccg tttttggggt acgaaatgac gaagccgaac ctgtgggcgt tcttgtgaag
       61 ccacttgctt tcgtcgagga cctcgaactc gcggacgttc ggatctccgt tcacgccgtc
      121 ctcattgatg aagtcgagcg cctggtattt tgtcgatccg tgctcgctgt agcccggcag
      181 cgcgacgaac ttgacggtct ccctgaccga ataatcatgg ttcttgagat agtagataaa
      241 cagatagagc tggaacgcgc tcgaccggta gccggattcc acatagaggc gcttgccgat
      301 gtccttttgc atggcggaca tcattttttc gtaagcgatg tagacatcag gcggaaggaa
      361 ttgcggcggc agtacccgcg tttgcagttt ggtcttcttt gtcccgtccg ggtttttgac
      421 gacgaccttt tccttcaccc gctggccctt gaggatcacg aggtcttcct tgcccgttgc
      481 gattccgcgc cacggaatcc ggacgcccgc gtcctttcgt gagatattcc ggaactcttt
      541 gacgaacgcg atctcctttt cgttgagcgg cgcatagagt tcgtcaaagg tcagtttcgc
      601 cagattcttt tcggaatcgc gtttccggat gaggggctcc agtttcgcga ttacctgctt
      661 ttgcatggcg atatcggcgt cggagagtct gtcgggatcg agagtccacg cggacggaac
      721 ggccgcaaaa aggagaaaga ccaccagcgc gatacgtgtc atttcaggac ggcccccacg
      781 gaaagccggc ggccgttcag gaagtcggcg gcgggcatga cgttcctgcc ttccggtttc
      841 acgcggaaga tccggagcgc gtccgcgccg gttgcgacca caacggaacc gcttttgtcg
      901 atcgagagga tcatgcccgg ttcggatgcc tcgcatggga cggggtcagc cgagacgatg
      961 gtcacgcgtt cagactggaa gaagaaacag gcgccgggcc acggtgcgag cccgcggacc
     1021 tttcgtccgg tcgtcacggc ggggtcggaa aaggagacga gcccgtcctc tttcgtgagc
     1081 ttgcgcgcga ccgttgcgcg ggactcgtcc tgcggaacgc ccatcagctc ccctttcgca
     1141 atcttcgcaa aaacggttgc gagcgcttcg gcggaaagct tcgcgagttc gtgcgagagc
     1201 tctgcggcgt tcatcgccgg atggatggga taacgggtct ggtaatagac atcgcccgcg
     1261 tccatttttt cggcaatgtc gatgatgctg acgccggtct ccgcgtcccc gttcaggatc
     1321 ggccagttga gcggagccgc gccgcggtaa agcggaatga gcgacgggtg gacgttgagc
     1381 cggtatcgcg ggatcgtgaa gagcgcgggc ggaatgatct tgccgtagga ggcgacgacg
     1441 aagatgtccg gcgcgagggc cattagtttc tgcaggacgg acggttcgcg aaggtccggg
     1501 aattccccgg cgggcagttg atgcttgtcc gcggcggcgc gcacggcggt cggttcgaga
     1561 tggagtcccc gccctttccg tttcggaggt gtcgtcacga cgaggacgac ctcatgaccg
     1621 gaggcgagac acgcttcgaa cggtttgagc gaaaaatcgg aagagccgaa gaagacaatc
     1681 ttcat
//
LOCUS       DAOOBX010000219         1679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_78974_length_1679_cov_3.084360, whole genome shotgun sequence.
ACCESSION   DAOOBX010000219 DAOOBX010000000
VERSION     DAOOBX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78974_length_1679_cov_3.084360"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..942)
                     /locus_tag="PLY30_03615"
     CDS             complement(91..942)
                     /locus_tag="PLY30_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HPW65228.1"
                     /translation="MDKVLTFFAAPLTAFFMPAVYRNAAKSSPARGLLYQLYLTGLVV
                     LVAAVALGATLMPKADGFAKWMGENLPPMIWTPEAGLSMESDQTPQPFLLEHPEFGAI
                     AIIDTKRIEPVRVEDMGDAYAFVTADKFYARNAAAKDGVEVRSLKTTPRPGSELPKRV
                     RITGDVVLKFYNNLRSVMAPMLLGVLLVGLYLFFIVLALLHSIAALVFDSIRKSDLGY
                     GALFNLSCFSMSTGFTVTWFNMLPFLRSIPIPVVVVFLINLGYLFFAVKVAGPKKEEG
                     PSVKTGA"
     gene            complement(935..1411)
                     /gene="greA"
                     /locus_tag="PLY30_03620"
     CDS             complement(935..1411)
                     /gene="greA"
                     /locus_tag="PLY30_03620"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPW65229.1"
                     /translation="MSEKVIVTRQGYEKMIEDLNALKTTKRREVADQLEKARAFGDLS
                     ENAEYETAKEAKHQLEIRIGELERKIAHARVVDLNDIPKDKVYLGATVKVQDLDNKEI
                     FRYTLVPQDEANFDEGKISVTSPIGKGLLGKVLNEEAAIQAPARTIKLKIMEITYG"
BASE COUNT          370 a          499 c          442 g          368 t
ORIGIN      
        1 cctgagcgga tcactgatct cctacctcga aaccagggtc aggacccgcg aggaggtctc
       61 cgcccttccc ggcgaaactt ccctctgacg ttatgcgccg gttttcacgg acggtccctc
      121 ttcctttttc ggacccgcga ccttgaccgc gaagaacaga taacccagat tgatcaagaa
      181 gacaaccacc acgggaatgg ggatcgagcg caagaacggc aacatgttaa accaggtgac
      241 cgtaaatccg gtactcatgg aaaaacagga caggttgaag agggccccat agccgagatc
      301 gcttttccgg atcgagtcga aaacaagcgc cgcgatggaa tgcaaaagcg ccaatacaat
      361 gaagaacagg tataatccga ccaggagcac gcccagcagc atcggtgcca tcacagaccg
      421 gagattgttg tagaacttga gaacaacatc accggtaatg cggacacgct tcggaagctc
      481 gctgcccggc cgaggtgtcg ttttgaggct tcggacttcg acaccgtcct tcgccgcggc
      541 gttcctggcg taaaacttat ccgccgtaac aaaggcataa gcgtccccca tatcttcgac
      601 cctgaccggc tcgatccttt ttgtgtcaat gatcgcgatc gcgccaaact cgggatgttc
      661 caaaagaaac ggttggggtg tttgatcgct ttccatcgac aaccccgctt cgggggtcca
      721 gatcatcggc ggaagatttt cacccatcca cttggcgaag ccatccgctt tcggcatcag
      781 agtggcgccc aaagcgacgg cagctacaag gacaacgaga ccggtaagat agagttgata
      841 aaggagcccg cgcgcgggcg aactcttggc ggcattgcga tagaccgccg gcataaagaa
      901 cgcggtcaag ggagcggcga aaaaggtcag gactttatcc ataggtaatc tccatgattt
      961 tgagtttgat cgttctggcg ggagcctgga tggccgcctc ttcgttcagg actttcccca
     1021 gcagaccttt cccgatcggc gaggtgaccg aaatcttgcc ctcatcaaaa ttggcctcgt
     1081 cctggggaac cagcgtgtac cggaaaattt ccttgttgtc gaggtcctgg accttgaccg
     1141 tggcgccgag ataaaccttg tccttcggga tgtcgttcag gtccacgacc ctcgcgtgcg
     1201 cgatcttccg ctcaagctcc ccgatccgga tctcaagctg gtgcttggct tctttggcgg
     1261 tttcgtattc cgcgttctcg ctcagatccc cgaatgcccg ggccttctca agctggtccg
     1321 cgacctcgcg gcgtttcgtg gtcttgaggg cgttgaggtc ctcaatcatt ttttcgtagc
     1381 cctgcctggt gacaatcact ttttccgaca taatccactc cgccggagat gtttttccgc
     1441 tttgaagtcg aaaccgtcca ttgttcgaga attcctggct acaccgtcga tacgcccgca
     1501 aaaaccatcc cgtccgccct ccggattctt accggagctc gaaggaactg tccgcgagaa
     1561 acggttcata gtccgcttcc ctgatacatc ggacgaggtc cccggaactt aagcccgccg
     1621 gggcgagact tcccgcttcc cgcatgacac gctcatgaag atttgttcct ccgatgtca
//
LOCUS       DAOOBX010000220         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_79106_length_1677_cov_2.849568, whole genome shotgun sequence.
ACCESSION   DAOOBX010000220 DAOOBX010000000
VERSION     DAOOBX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79106_length_1677_cov_2.849568"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..>1677
                     /locus_tag="PLY30_03625"
     CDS             49..>1677
                     /locus_tag="PLY30_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65230.1"
                     /translation="MASGFVLMAAACFAGIILIRFSRSVLGIVPGALPDLLPALLYSF
                     ATIGPVCFLLGMQFSGLAKMSVGTEEPDAGGVLGRGYLFESLGFMAGGILFSLFFVRL
                     NVFYAMSVIAALNFGTAAMLAFPGNIKKTAVAFLMALAAVTVMGLFADRLDLATAALR
                     FPSEKLEITRNTVHGNISVTSSGAQRNFYQNGALTGADREDLASELLVHFPLLAHPSP
                     GRVLIFGTGFNGPVREALKHRPREIVSVDIDPELVRIAKGRLPRDLEKSLEDPAVLLA
                     SGDPRAYLSSVRGTGDVVLLNFPDPSSVLVNRNFTLEFFRSVRDRLAPDGIFALKLDF
                     APNYISRELERLGSSVYATLRQVFPSVVVLPEDTAYFLASPREGWVPDADDMAERFMA
                     RGLRTEFVTPSLVRDRLTNDRVQQVVTIFRNSVYKVKNTDFRPRACYFALMRWLSRFR
                     PAASQALLRVSDLPFGLVAAVFLILFHLPFAGGSDDPAQRYRKLGCRAMSVTGFSVMA
                     FEVALIYLFQVAFGDLYYRIAWLIAALTNVRATAT"
BASE COUNT          255 a          493 c          529 g          400 t
ORIGIN      
        1 accgccgccg gcagtggctt ggcggggaaa cgcacccgcc cgccggtctt ggcgtccggt
       61 tttgtgctca tggccgcggc ctgttttgcg gggatcatcc tgatccgctt cagccggtct
      121 gtgctgggga tcgtccccgg tgctttgccg gaccttctgc ccgcacttct ctattctttc
      181 gcgacgatcg gtcccgtttg cttcctgctc gggatgcaat tctcggggct cgcgaagatg
      241 tctgtgggaa cggaggagcc ggacgccgga ggggtcctcg ggcggggata tctttttgaa
      301 tcgctcgggt tcatggccgg gggaatcctg ttcagccttt tctttgtccg tttaaacgtg
      361 ttctacgcga tgagcgtgat cgcggccctg aacttcggga ccgcggcgat gctggcgttt
      421 ccagggaaca tcaaaaaaac cgctgtggct tttttgatgg ctctcgccgc ggtgaccgtc
      481 atgggtctgt tcgcggaccg gctggatctt gccacggcgg cattgcgatt tccgtctgaa
      541 aagctggaga tcacgcgcaa tacggtccac gggaatattt ccgtcacgtc ttccggagcc
      601 cagcgcaatt tttaccagaa cggcgcgctt accggggctg accgggagga tcttgcgagt
      661 gaactcctcg ttcattttcc cctgctcgcg catccttcgc cgggccgcgt gctgatcttc
      721 ggaacgggat tcaacggtcc ggtccgggag gccttgaagc accgcccccg ggagatcgtt
      781 tccgtggata tcgatcccga actggtgagg atcgcgaaag gacggttgcc ccgggacctt
      841 gaaaagagcc ttgaggatcc cgcggtgctt cttgcgagcg gcgatccgag ggcatacctc
      901 tcgtccgtcc gggggactgg cgatgtggtg ctgttgaact ttccggaccc ttcctcggtg
      961 cttgtgaacc ggaacttcac gctggaattt ttccgttcgg tccgcgaccg tctggcaccg
     1021 gacggtattt tcgctctgaa actggatttc gcgccgaatt acatcagccg cgagctggaa
     1081 cgcctcggaa gttccgttta tgcgacgctc cggcaggtat tcccgtcggt cgtggtcctt
     1141 ccggaagata ccgcgtattt tctcgcatca ccgcgggagg gttgggtgcc ggacgctgat
     1201 gatatggccg agcggttcat ggcgcgaggc ctgcgaacgg agtttgtgac cccctcgctg
     1261 gttcgcgacc ggctcaccaa cgaccgcgtc cagcaggtcg taacgatttt caggaattcg
     1321 gtgtacaaag tcaagaacac ggatttccgg ccgcgggcct gctatttcgc cctgatgcga
     1381 tggctgagcc ggttccgccc cgctgcctcc caggcgctgc tgagggtctc ggatctcccg
     1441 ttcggccttg tcgccgccgt attcctgatt ttgtttcacc ttccattcgc gggagggagc
     1501 gatgatcccg cccaaaggta ccggaaactc ggttgccggg cgatgagtgt gaccgggttc
     1561 tcggtcatgg ccttcgaggt ggcattgatc tacctctttc aggtggcttt cggggacctc
     1621 tattacagga tcgcctggct gatcgcggcg ttgacgaacg tcagagcgac ggcgacg
//
LOCUS       DAOOBX010000221         1658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_80372_length_1658_cov_2.269495, whole genome shotgun sequence.
ACCESSION   DAOOBX010000221 DAOOBX010000000
VERSION     DAOOBX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80372_length_1658_cov_2.269495"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLY30_03630"
     CDS             <1..450
                     /locus_tag="PLY30_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPW65231.1"
                     /translation="NRILTQNREKLEMTVPISQSVPISYNTWLVKNYMDTVSRSLDEA
                     ARIDGAGHFRIYSTIIMPVARPIITFLGITSFTAPWMDYIFPKMVLRSVEKQTLALGL
                     FSFVTDKKNDFTTFAAGSLIVAIPFIAFYIITQKAMITSFGGAAVKE"
     gene            463..>1658
                     /locus_tag="PLY30_03635"
     CDS             463..>1658
                     /locus_tag="PLY30_03635"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HPW65232.1"
                     /translation="MKHAIYALCIGAVAFAGCASSGVSSGNAAASLPVYSGNPEEGEL
                     FVEKIEGLPAAFLRGVDVSSVLALEKSGVVFRDKAGNPQDLFVTLRDSGVNSIRIRVW
                     NNPFDDQGRGFGGGNCDIDAAIVMGRRAAAAGLPVLLDFHYSDFWADPAKQQAPRAWA
                     GMDIDAKADALYEYTKTSLSKAVKAGVNVHMVQIGNETTGAMCGETNWINIAKLMRAG
                     SKAVREVSAKEKKNIKIAVHFTNPEKSGEYERYVQILSKQNLDYDVFASSWYPWWHGS
                     VDNFAAVLKRVAELSGKEVMCAEVSYAHTYENGDETGNTISEETMCEKPWPITVQGQA
                     SAVRETIAAVASVGKAGIGVYYWEPAWLPVPGETYEDQRLLWDRDGSGWASSYASAYD
                     PKDAGK"
BASE COUNT          373 a          466 c          472 g          347 t
ORIGIN      
        1 aaccgaattt tgacccaaaa ccgcgaaaaa ctcgaaatga cagttcctat cagtcagtca
       61 gttcctatca gttacaacac ctggctggtt aaaaactaca tggatactgt atcccgcagc
      121 ctcgacgaag cggcccgcat cgacggagcc ggacacttcc gcatttactc gaccatcatc
      181 atgccggtcg ctcgccctat catcaccttt ctcggcatca cgagcttcac cgctccgtgg
      241 atggactaca tcttcccgaa gatggttctc cgctccgtcg aaaagcagac tctcgctctc
      301 ggtctgttca gtttcgttac cgacaagaaa aacgatttta ccacgttcgc cgccggctcc
      361 ctcatcgtcg ccataccctt catcgcgttt tacatcatta cgcaaaaggc tatgattacg
      421 agcttcggcg gagcggcagt caaggaataa gaaggagttt ttatgaaaca tgccatttac
      481 gctctctgca tcggggccgt cgcgttcgcc ggctgcgctt cctccggcgt ctcttccgga
      541 aacgcggccg cgtctctgcc cgtctactca ggaaatcccg aggaggggga attgttcgtg
      601 gaaaagatcg aaggcctgcc tgccgccttc ctgcgaggcg tcgatgtgtc gagcgtttta
      661 gcgctcgaga aaagcggggt cgtgttccgg gacaaggccg gcaatccgca ggatctgttc
      721 gtaacgctcc gggattcggg cgtgaacagc atccgcatcc gcgtgtggaa caatcccttc
      781 gacgatcagg gccggggctt cggcggcggc aactgcgaca tcgacgcggc gatcgttatg
      841 ggaaggcggg ccgcggccgc agggcttccg gtgcttcttg attttcatta ttccgacttt
      901 tgggccgacc ccgctaaaca gcaggctccg cgagcttggg cggggatgga tatagacgcc
      961 aaggccgatg ccctctacga atacacgaag accagtctgt caaaagctgt caaggcgggc
     1021 gtgaacgttc acatggttca gatcggcaat gaaaccaccg gcgccatgtg cggcgagacc
     1081 aactggatca atatcgcgaa gctcatgagg gccggaagca aagcagtccg cgaagtttcg
     1141 gcgaaagaga agaagaacat caaaatcgcc gtgcatttta cgaatccgga aaagtcaggc
     1201 gagtacgaac ggtatgttca gatcctgtca aagcagaatc ttgattacga cgtgttcgct
     1261 tcctcctggt atccgtggtg gcacggttcg gtcgacaatt tcgcggccgt gctgaaacgc
     1321 gtcgccgaac tttccggcaa ggaagtgatg tgcgccgaag tttcgtacgc gcatacgtat
     1381 gaaaacggcg acgaaaccgg caacacgatc tcggaagaaa caatgtgcga aaagccgtgg
     1441 ccgatcaccg ttcagggtca ggcatcggct gtgcgcgaaa cgatcgccgc cgtggcttcg
     1501 gtcggaaagg cgggaatcgg cgtgtactac tgggagcccg cgtggcttcc tgtgccgggc
     1561 gaaacgtatg aagaccagcg gcttctttgg gatcgcgatg gttccggctg ggcttcaagt
     1621 tatgcctctg catacgatcc gaaggacgcc gggaaata
//
LOCUS       DAOOBX010000222         1657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_80410_length_1657_cov_3.762172, whole genome shotgun sequence.
ACCESSION   DAOOBX010000222 DAOOBX010000000
VERSION     DAOOBX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80410_length_1657_cov_3.762172"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1143
                     /locus_tag="PLY30_03640"
     CDS             178..1143
                     /locus_tag="PLY30_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPW65233.1"
                     /translation="MKNIRKIGREILDIETKALLRLRPKIGKNFEKAVRMMANCKNRV
                     IMTGMGKPGLIARKLSATLASTGTPSLYLHPAEAVHGDLGMVTSQDLIIAISQSGETA
                     EIVRLLPILKKKGVPLIAMTSMPKSTLARHAEVVLDLGVTKEACPLNLAPSASTTAAL
                     AMGDALALTLLKIKKFRPEDFAALHPGGSLGKRLLRVGDIMRKGKANPLVRKGTSVRE
                     ALLRITAARAGSCTVVDPKGKVVGIFTDGDLRRHLREKGEALLSLPIETVATPKPRVI
                     ARDKLAEEAFHILESLRIDEIPVVDEKSRVVGLLDVQDLMRAGIV"
     gene            1275..>1657
                     /locus_tag="PLY30_03645"
     CDS             1275..>1657
                     /locus_tag="PLY30_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65234.1"
                     /translation="MARYKPYRYPLYLLARAAAALPLLVPRSWALAFARAAGWLGYVM
                     IPRQRKRMIQNLGFAYGDEKSPEEIRRIAARVMGNLLETFVDFLRFAKLTREKASRFI
                     DAGDAYDRCKDILQEGKGLIIMTPH"
BASE COUNT          355 a          437 c          538 g          327 t
ORIGIN      
        1 gacgctcgcg cgttgcgcgg tcgttgcggg tgcggacgcc gtgttcatgg aaatccaccg
       61 ggacccgtcg cgggcgcttt cggacggagc gaacacgatc gcgctcaaga atctgaagcg
      121 gcttctcacg gatctgatcg ctctcaaaag aaccgttacg gcgggaggga tggtgtgatg
      181 aaaaatatcc ggaaaatcgg ccgggagatt ctcgatatcg agacgaaggc gcttttgcgg
      241 ctccggccaa agatcgggaa gaatttcgag aaagcggtcc gcatgatggc gaattgcaag
      301 aaccgcgtca ttatgaccgg gatgggaaaa ccggggctca tcgcgcggaa actttccgcc
      361 acgctcgctt cgaccggcac accgagcctt taccttcatc ccgcggaagc cgttcacggc
      421 gatctcggta tggtcacgtc ccaggacctc atcatcgcga tctcccagag cggagagacc
      481 gcggagatcg tgaggctcct gccgatcctc aagaaaaaag gcgtgccgct cattgcgatg
      541 acgagcatgc cgaaatccac gctggccagg cacgcggagg tcgtgctcga tctcggcgtt
      601 acgaaagagg cttgtcctct caatctcgcg ccttcggcgt cgacgacggc ggcgctcgcg
      661 atgggggatg cgctggcgct cacgcttctc aagatcaaaa agttccgccc ggaggatttc
      721 gcggcattgc accccggcgg gagtctcggg aaaaggctcc tcagggtcgg ggacatcatg
      781 cggaaaggaa aagcgaatcc gctggtccgc aaggggacct cggtgcgcga ggccctgctc
      841 aggatcaccg ccgcgcgcgc gggaagctgt acggtcgtgg acccgaaggg gaaggttgtc
      901 gggattttta cggacggcga cctccggcgg catttgcgcg aaaaggggga ggcgcttctc
      961 agtctcccga tcgaaaccgt ggcgaccccg aaaccgcgcg tcatcgccag ggacaagctt
     1021 gcggaagagg ccttccatat tcttgagtcc ctcaggatcg acgagattcc ggtggtggat
     1081 gaaaagtccc gcgtggtcgg gcttttggat gttcaggatc tcatgagggc ggggatcgtc
     1141 tagcaagatc aagcgcggaa cccggaataa cagcacaagg aaggcgagga aagttcccga
     1201 gggattgttc gggaaaattg agtgttcggg ttttggattt gtttcgaacg ccggacaaaa
     1261 aactttggat ctgaatggcc agatataaac cataccgtta tccgttgtat ttgttggcaa
     1321 gggctgcggc ggcgttgcct cttcttgtgc cgcgaagctg ggcccttgcg ttcgcgagag
     1381 cggcggggtg gctgggatat gtcatgatcc cccgtcagcg gaagagaatg atccaaaatc
     1441 tcgggtttgc ctacggggat gaaaagtccc cggaggagat ccggcggatt gcggctcgcg
     1501 ttatggggaa cctgcttgag acctttgtgg atttcctgcg gtttgccaag ctgacgaggg
     1561 aaaaggcctc ccggtttatc gatgccggtg acgcttacga ccggtgcaaa gatattttgc
     1621 aagaaggaaa ggggctcatt ataatgacac cccactt
//
LOCUS       DAOOBX010000223         1656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_80500_length_1656_cov_2.891318, whole genome shotgun sequence.
ACCESSION   DAOOBX010000223 DAOOBX010000000
VERSION     DAOOBX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80500_length_1656_cov_2.891318"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="PLY30_03650"
     CDS             complement(<1..1633)
                     /locus_tag="PLY30_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65235.1"
                     /translation="MLKEMKISDAGLSSSISNLYVDTMLIRTAASYLVNVLDLASIGT
                     QILSAQTGENGRIMVETALGRMTLDPQAVSLLHSASGGRLAAYETFVLKFLKDRSIES
                     LKAELGKSETSVTRRSEVSRLISELGKLETLDALKGQIKLAETLLVELQTYFYGVEKG
                     RGIFWGEEFTRSLLILGFTDIAQKLFDVVSLKGDKGGDYIFIKTEAGERHAALVDGKK
                     SFERLGTPAAQAEIARIDRELKALESQSLEVLVSNVANADPVRDAVRLLGQTITDRGV
                     VTDVGRFIDFLRQAFVGKQAGVYYDFFRAVDRIAESVKTTVESGNSVDLRSVEASVSV
                     AVRDLFRLDAASLKALEPQLRQIAEALVSRDTSTFYKDVFGALDVAAFRSRISDYLLG
                     QLSMGRGLGFEAGRASDFLSDLGQLVKVSERFSGDRAQKTLSEWRSAAMGSEQRKFIE
                     GYDRAMDGKTVHVEGFESLIKAGRIEDAYRSYEHLFGGALAPAVQESFFRIFREVIAQ
                     LNPSLYMKVFARVEKGDSGFQMFDPRAVLTAEEMSA"
BASE COUNT          400 a          499 c          439 g          318 t
ORIGIN      
        1 cggcggacat ctcctccgcg gtcaatacag cacgggggtc gaacatctgg aaaccgctgt
       61 cccccttctc gacgcgggca aaaaccttca tgtaaagcga tggattcaac tgcgcgatca
      121 cctcgcggaa gatacggaag aaagattcct gaacggcggg agcaagcgca ccaccgaaaa
      181 gatgctcgta agaacgataa gcgtcttcga tcctgccggc cttgatcaac gattcgaaac
      241 cctcgacatg gaccgtcttg ccgtccatag cccggtcgta accctcgata aacttccgct
      301 gttctgaacc catcgcggcc gagcgccatt cggatagtgt tttttgagcg cgatcgcccg
      361 agaagcgctc gctgaccttc acaagctggc cgaggtccga gaggaaatcc gaggcgcgac
      421 ccgcctcgaa accgagaccg cggcccatcg aaagctggcc gagaaggtaa tcgctgatcc
      481 ggctccggaa cgcggccacg tcgagggccc caaaaacgtc cttgtaaaaa gttgacgtgt
      541 cgcgggacac gagagcttcc gcgatctggc ggagttgcgg ttccagagcc ttcaaagacg
      601 cggcatcaag cctgaaaagg tctctgacgg ccacagaaac ggaagcctca acggatctca
      661 gatcaacgga gttcccgctt tcaaccgtcg ttttcacact ttccgcgatg cggtccacgg
      721 ccctgaagaa atcataataa acaccggcct gtttaccgac gaacgcctgc cgcaggaaat
      781 cgatgaaacg gccgacatcg gtcaccacgc cccggtccgt gatcgtctga ccgagaagcc
      841 tcacggcgtc ccgaacagga tcagcattcg caacattcga aaccaacact tctaaagact
      901 gcgattcaag agctttaagc tcgcgatcga tcctggcgat ctccgcttga gctgcaggtg
      961 ttccgagccg ttcaaaggat ttcttgccat cgaccaatgc ggcatgccgt tcaccggctt
     1021 cggtcttgat gaaaatataa tcgccgcctt tatcaccctt cagagaaaca acgtcgaaaa
     1081 gtttctgggc gatatcggta aaaccaagga tcagaagcga ccgggtgaac tcctcgcccc
     1141 agaagatgcc ccggcccttt tcaacaccgt agaagtaggt ctggagttca acgagaagcg
     1201 tttcggcgag cttgatctgc cctttaagag cgtcgagggt ctccaactta ccaagctccg
     1261 agatcaaacg cgacacctcg gaacgacgcg ttaccgaggt ctcactcttg ccgagctccg
     1321 ccttaagaga ttcgatcgaa cggtctttga gaaatttcag aacgaaggtc tcgtacgcgg
     1381 cgagccggcc gcccgaagcg ctgtgcagga ggctgacggc ctgaggatcg agcgtcatcc
     1441 ggcccaaagc cgtttccacc atgatgcgcc cgttctctcc tgtttgggcg ctgaggatct
     1501 gcgtcccgat cgaagcgaga tccagaacgt tcacaagata gcttgccgcg gtacggatca
     1561 gcatggtatc cacatacagg ttgctgatcg aagaagaaag gccggcatcc gagatcttca
     1621 tctctttcaa catctcaccg acacggcggc cggcgt
//
LOCUS       DAOOBX010000224         1650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_80880_length_1650_cov_3.581818, whole genome shotgun sequence.
ACCESSION   DAOOBX010000224 DAOOBX010000000
VERSION     DAOOBX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80880_length_1650_cov_3.581818"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLY30_03655"
     CDS             <1..683
                     /locus_tag="PLY30_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW65236.1"
                     /translation="VTICNDLGIPLVFFSTDYVFDGKKKGEYLESDPANPVSVYGRTK
                     KDAEDHILANAKSAVIFRITWLYGVFGRSFPRAILRQIAETTKLTVVADQIGRPTYTK
                     DIAQALKVLLSSRRDKVFAPGARIYHLTNTGQVTWADFARFILERVGRDDVTVEDMTS
                     DKLNRPAPRPANSLLNLEKSERELGVILRPWQDAVAEFLVELEEVRKAAEAKARAKAE
                     AEGASDQG"
     gene            684..1637
                     /locus_tag="PLY30_03660"
     CDS             684..1637
                     /locus_tag="PLY30_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW65237.1"
                     /translation="MMDKKTVIVTGGGGFLGSHLCDRFLARGFRVFAIDNFLTGNEKN
                     IAHLSGNGDFEVIRHNVCEYIDFPGPVDYILHFASPASPLDYLQLPIQTLKVGALGTH
                     NALGLAKEKKAVFLLASTSEVYGDPVVHPQKESYWGNVNPVGPRGVYDEAKRYAEALT
                     MAYHRTHKLDTKIVRIFNTYGPRMRLHDGRAIPAFLSQALRGEPLTVFGNGKQTRSLC
                     YIDDMVNGIEKLLMSAENEPMNIGNPEEISMMDLAKRIIKITGTSSGIAHEELPVDDP
                     KIRQPDITKAKKQLGWYPTISLEEGLKRTIEYFKQALAESK"
BASE COUNT          391 a          470 c          463 g          326 t
ORIGIN      
        1 tcgtgaccat ctgcaatgat ctcggaatcc cgcttgtttt cttcagcacc gattacgtct
       61 ttgacgggaa gaaaaaagga gagtacctgg aatccgatcc cgcaaatcca gtcagtgtct
      121 acggccggac caaaaaggac gcggaggatc acattctcgc gaacgcgaaa agcgctgtca
      181 tcttccggat cacgtggctt tacggagttt tcggccgctc attcccgcgt gccatcttgc
      241 gtcagatcgc ggagacaacg aaactgaccg tggtagcgga tcagatcggt cggcccacgt
      301 ataccaaaga catcgcgcag gcgctcaagg tcctcctttc gtcccgcaga gacaaggttt
      361 tcgcgcccgg tgcccgtatt tatcacctga caaacacggg acaggtcacc tgggcggatt
      421 ttgcgcggtt catacttgag cgcgtgggtc gcgatgacgt aaccgtcgag gatatgacct
      481 cggataaact gaaccgcccg gcgccgcggc cggcaaattc gttattgaac ctcgaaaagt
      541 ccgagaggga acttggcgtg atccttcgac cctggcaaga cgcggtcgcc gagttcctcg
      601 tcgagctgga agaggtccgc aaagccgcgg aagccaaagc cagggcgaaa gccgaggcgg
      661 aaggcgcgag cgatcaggga tagatgatgg ataaaaaaac agtgatcgtc acgggcggcg
      721 gggggttcct cggatctcat ctgtgcgacc ggttcctcgc tcgcgggttc cgggtgttcg
      781 cgatcgataa tttcctcacc ggaaatgaga agaatatcgc gcatctttcc gggaacgggg
      841 atttcgaggt tatccggcac aatgtctgcg aatatatcga tttcccgggg ccggtggatt
      901 atatccttca tttcgcgtca cccgcgagtc cgcttgatta tcttcagctc ccgatccaga
      961 cgctgaaggt cggggctctc ggcacccata atgccctcgg cctcgccaag gaaaagaaag
     1021 ccgtgttcct gctcgcgtcc acttccgagg tttacggcga tcccgtcgtc cacccgcaaa
     1081 aggaatccta ttggggaaat gtgaaccctg tcggcccgcg cggggtctat gacgaggcca
     1141 aacgctacgc ggaagccctg accatggcct atcaccgcac ccacaagctt gacacgaaga
     1201 tcgtccgcat cttcaacacg tacggcccga ggatgcgcct ccatgacggc cgcgcgatcc
     1261 cggcgttcct gagccaggcc ttgcggggcg aaccgctcac cgttttcggt aacggcaagc
     1321 agacccgcag tctgtgttat atcgacgata tggtgaacgg gatcgaaaaa ctcctcatgt
     1381 ccgccgaaaa tgagccgatg aatatcggga atccggagga gatttcgatg atggacctcg
     1441 cgaagaggat catcaagatc accggaactt caagcgggat cgctcatgag gaacttccgg
     1501 tcgatgaccc gaaaatccgg cagcccgata tcacgaaagc aaaaaagcag ctcggatggt
     1561 accccacaat ctcacttgag gaaggcttga aacgcactat cgaatatttc aaacaagcgc
     1621 tcgcggagag taaataatca tggcccctta
//
LOCUS       DAOOBX010000225         1645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_81233_length_1645_cov_2.759119, whole genome shotgun sequence.
ACCESSION   DAOOBX010000225 DAOOBX010000000
VERSION     DAOOBX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81233_length_1645_cov_2.759119"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="PLY30_03665"
     CDS             <1..169
                     /locus_tag="PLY30_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPW65238.1"
                     /translation="PGECLTIMTAGAYGFTMSSNYNSRGRACEVMVKGSSFRIVRKRE
                     TYQDLVRGECV"
     gene            379..1203
                     /locus_tag="PLY30_03670"
     CDS             379..1203
                     /locus_tag="PLY30_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW65239.1"
                     /translation="MTTHERRTHKGEKLPGEVAIYCESRGEGAPLLLIAGLGTDSSNW
                     NGVIARLPKQYRILTLDNRGAGRSSIPGKPYSVIEMAEDVLRVLDHFKVGRAHVLGHS
                     LGGFIAQELAIRYPERVDRLVLSSTAAVSSGRNNELFLKFYRDLAQGGDLEAWLREWT
                     RWLFSPSVLSRESFVAALVKRGIRDRYRQQLPGFKGQIDAVASFDARGRLGGIKARTL
                     VLEGGEDVLILPEEAEALSKMIPGSVYRCIPAVAHSIHMEEREVFVNIVHGFLAAE"
BASE COUNT          316 a          415 c          556 g          358 t
ORIGIN      
        1 gcccggcgaa tgcctcacga ttatgaccgc gggtgcctac ggttttacga tgagttccaa
       61 ctacaattcc cgcggtcgcg cctgcgaggt gatggtgaag ggaagctcct tcaggatcgt
      121 ccgcaaacgc gagacctacc aggacctggt tcggggtgaa tgtgtttagg gtttttgaaa
      181 atcggggccg tgccggcgtt tcggcacggc cttttttgtt gaaaccatag tagatattct
      241 ttgtggtttt ccggcagaaa ctggttgtta gccattgatc cgggattttt ccaaaaacag
      301 gattgttttt gttgaaaaac gaacttgatc gaccgggaat tagcgggact agagtctaaa
      361 agacagactg tgaaacccat gaccactcac gaacgacgaa cgcataaggg ggagaaactt
      421 ccgggcgagg tcgcgatcta ctgcgagtcg cgcggggagg gggcgccgct tcttttgatc
      481 gcggggcttg ggaccgacag ctcgaactgg aacggggtga tcgcgagact tccgaagcag
      541 taccgcatcc tgacgctcga caaccggggc gcggggcgca gttcgattcc gggaaagccg
      601 tacagcgtca tcgaaatggc ggaggacgtc ctgcgggtgc tcgatcattt caaggtcggg
      661 cgggcgcatg ttctgggtca ttccctcgga ggttttattg cccaggagct tgcgatccgg
      721 tatccggagc gcgtcgaccg gctcgtcctg tcgagcaccg cggccgtttc ttccgggagg
      781 aacaacgagc ttttcctgaa attttacagg gaccttgcgc agggggggga tctcgaggcg
      841 tggctccgcg aatggacccg ctggcttttt tcgccgagcg ttctttcccg ggaaagtttc
      901 gttgcggcgc tcgtgaaacg gggcatccgc gatcgttacc ggcagcagct tcccggattc
      961 aaggggcaga ttgacgcggt cgcttcgttc gacgcacgcg ggcgtctcgg agggatcaag
     1021 gcccggacgc ttgttctcga gggcggggag gacgtgctga tccttccgga agaagcggag
     1081 gcgctttcga agatgatccc tggcagcgtt taccggtgca tccccgccgt ggcccactcg
     1141 atccatatgg aagagcgcga ggtgttcgtc aacatcgtcc acggatttct ggcggcggag
     1201 taatagggaa aagagcgaac atcgcatcgg tgtgatgcgg tgcaaacggt ccccgcagag
     1261 ctttttcctg cctgtgctga tccctgacct ggtcttctcg gggtatgttc acgcgcgggc
     1321 ggaggatcgg ggatcggagg gttgttgcca ggttgtgatc gagaatgctt gaagcatgcc
     1381 tttgtggaat gcgaagggaa ttccgcccgc gagtatcagg tccgaagctg aacaggattc
     1441 gtgagtcaaa cgccgcaacg ggtaaccccg ggtgcggttt ttatttcgag tagggcaggt
     1501 cggggaccgg cgtcgcgaca agagtcggcg gttcgggaac cccggaagta tcctgggccg
     1561 cggtttccgc tagagaagtc gtgggaacca ttccaacctc gtaggttgga gtggtcgggg
     1621 gagtctggat cggcggcgga acggg
//
LOCUS       DAOOBX010000226         1638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_81696_length_1638_cov_3.037271, whole genome shotgun sequence.
ACCESSION   DAOOBX010000226 DAOOBX010000000
VERSION     DAOOBX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81696_length_1638_cov_3.037271"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLY30_03675"
     CDS             complement(<1..408)
                     /locus_tag="PLY30_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPW65240.1"
                     /translation="MNKVTAGKIVFGDPKKLVLIGGPCVIEDEPGTMKLAAAIRKLAA
                     GLRMPYVFKASFDKANRSSIRSFRGPGIQKGLRTLAKIKKEFGLSVLTDIHTPDQAEP
                     VAEVADILQIPAFLCRQTDLLVAAARTGRVVNVK"
     gene            complement(401..>1638)
                     /locus_tag="PLY30_03680"
     CDS             complement(401..>1638)
                     /locus_tag="PLY30_03680"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPW65241.1"
                     /translation="IESLPFLEAIRQFRHEVGRENSLFVHLTLVPYIRAAGELKTKPT
                     QHSVGTLREIGIQPDILICRTEKKIDQPMKAKIALFCNVEPEAVIEARDVTSIYECPL
                     MFKAEGIDKVICRKLRIEYKSGSLHAWKKEVVDIALRPRFSVKVAVVGKHTEIQDAYK
                     SVYESLKHGGIANDSQVIIKRVNSDKPGTARSLSQLKDVSGILVPGAFDERGIEGKLL
                     AIRYAREKKVPFFGICLGMQCAVIEFARNVLGMKGANSTEFNKKSPFPVISLSGEQEK
                     AKDPGGTMRLGAYPCDVRKDTKAYRAYKAEQISERHRHRYEFNNEYRERFQKGGMKIS
                     GISPNGKLVEMVELESHPWFVACQFHPEFKSKPDLAHPLFRDFIASALEFQKSNFPKS
                     ALKPVPAPTEVEEKTAAHE"
BASE COUNT          319 a          471 c          472 g          376 t
ORIGIN      
        1 tttcacgttc acaacgcgtc cggtccgggc ggctgcgacc agaagatcgg tctgccggca
       61 aaggaacgcc ggaatctgga ggatgtccgc tacctccgcc acgggttccg cctgatcggg
      121 agtatggatg tcagtcaata cggaaagccc gaactctttt ttgatcttcg cgagcgtcct
      181 gagtcccttt tgaatgccgg gaccgcggaa ggaccggatc gacgagcggt tcgccttgtc
      241 gaaactcgcc ttgaaaacat acggcattct gagccccgcg gcaagtttcc ggatcgcggc
      301 ggcgagcttc atcgttccgg gctcgtcttc gatcacgcaa ggccctccga tcaggaccag
      361 ttttttcgga tctccaaaaa cgatcttgcc ggcagtgact ttattcatgg gcagctgttt
      421 tttcctcgac ttccgtcggt gcgggaacgg gtttaagggc agatttcgga aaattcgatt
      481 tctggaattc cagggcgctc gcgatgaaat cacggaaaag cggatgcgcg aggtccggct
      541 tggatttgaa ctcgggatga aactggcaag cgacgaacca cggatggctt tccagctcga
      601 ccatctcgac cagctttccg ttcggggaaa tgcccgagat cttcatgccg cctttctgga
      661 acctctcgcg gtattcgtta ttgaactcat aacggtggcg gtgccgctcg gagatctgct
      721 cggctttata ggccctgtac gccttcgtat ccttcctgac gtcgcaggga taggccccga
      781 gacgcatcgt gcctccgggg tccttcgcct tttcctgctc cccggaaagg ctgatgacgg
      841 ggaacgggga tttcttgttg aactcggtcg agttggcgcc cttcatgccg aggacgttgc
      901 gggcgaactc gatcaccgca cactgcatcc cgagacagat cccgaagaac gggacctttt
      961 tctcgcgggc gtaccggatc gcgagcagtt tcccttcgat cccgcgctcg tcgaacgcgc
     1021 cggggacgag aataccggaa acatctttca actggctgag cgaacgcgcc gtcccgggtt
     1081 tgtccgagtt gacgcgcttg atgatgacct ggctgtcgtt ggcgataccg ccgtgtttca
     1141 gcgattcata gaccgacttg taagcgtcct gaatctccgt gtgtttcccg accacggcaa
     1201 ccttcacgga aaaccgggga cgaagcgcga tatccacgac ctcttttttc caggcatgca
     1261 ggctcccgga tttatattcg atccggagct tccggcagat caccttgtcg atcccctcgg
     1321 ccttgaacat gagggggcat tcgtagatcg atgtcacatc ccgggcctcg ataacagcct
     1381 caggctcgac attgcagaaa agcgcgatct tcgccttcat cggctgatcg atcttctttt
     1441 ccgtgcggca gatgaggata tccggctgga taccgatctc gcggagtgtg ccgaccgaat
     1501 gctgcgtcgg ttttgtcttg agctcgcccg cggcccggat atagggcacg agcgtgagat
     1561 ggacaaaaag ggagttttcg cggccgacct catgacggaa ctgacggatc gcctcaagaa
     1621 acggcagtga ttcgatat
//
LOCUS       DAOOBX010000227         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_81846_length_1636_cov_2.287793, whole genome shotgun sequence.
ACCESSION   DAOOBX010000227 DAOOBX010000000
VERSION     DAOOBX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81846_length_1636_cov_2.287793"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLY30_03685"
     CDS             <1..190
                     /locus_tag="PLY30_03685"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPW65242.1"
                     /translation="GFGSCWIGWFDEKALKRELAVPKDKRVDIVIALGYPAEEKAPVK
                     LRKFMEEISASNQYDCPI"
     gene            312..596
                     /gene="gatC"
                     /locus_tag="PLY30_03690"
     CDS             312..596
                     /gene="gatC"
                     /locus_tag="PLY30_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPW65243.1"
                     /translation="MKFNIDQVASLARLNLKPEEKPKLEKDLEAILDYIRNLEKLDTK
                     NVEPTSHVLDLKNVCRRDEAASSDAATKALEHAPHADGRFFKVPKIIQKD"
     gene            663..>1636
                     /locus_tag="PLY30_03695"
     CDS             663..>1636
                     /locus_tag="PLY30_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPW65244.1"
                     /translation="MKVKNMDLTQLTLKEAVEFAKKDGAGPILDAFEKRAAELNPGIN
                     AFLKMPCAMETGSRTCPEGVPGTFYGVPVSVKDNICIEGVEVTCASKILEKHIPPYDA
                     TVITKLRNAGVTLWGRCNMDEFALGSSCETSAFGPCKNPWDITRVPGGSSGGSAAAVA
                     ADMTLAALGSDTGGSIRQPAALCGVVGVKPTYGRVSRYGLIACSSSFDQIGPLAKTVE
                     DAALLMNVISGHDPKDSTSSPQPVPDFTKSLVRDVKGLRVGLPREYFIEGIDPEVEKS
                     VRAAVDLYRKLGAQIQEISLPHTEYSVAVYYIVAVAEASSNLSRFDGV"
BASE COUNT          398 a          452 c          466 g          320 t
ORIGIN      
        1 cggtttcggg agctgctgga tcggatggtt tgacgagaaa gccctgaaaa gagaactggc
       61 ggtcccgaaa gacaagaggg ttgatatcgt gatcgcactc ggatatccgg ccgaggaaaa
      121 ggctccggtg aaactcagaa agttcatgga agaaataagc gcttccaatc aatacgattg
      181 ccctatttga cacgtcccgt gtcttcaggc agaatgtgtt ttttgtcaag gaggactcgc
      241 acctcgcatg aaaaagcgag ttcccgggaa caagtgtcaa gtcccggtac atggtgaagg
      301 ataacccgtt tatgaaattc aatatcgatc aggtcgcgtc gcttgcgcgg ctcaacctca
      361 agcccgaaga gaaacccaaa ctcgagaaag accttgaggc gatcctcgat tacatcagga
      421 atctggagaa actcgacacg aagaacgtcg agccgacaag ccacgttctg gacctcaaaa
      481 acgtttgccg cagggatgag gcagcatcct ccgatgcagc gacgaaggcg ttagaacacg
      541 ctccccacgc cgacggccgc ttcttcaaag tcccgaaaat catacaaaaa gactagcgga
      601 gactgactct cccgccaaag gcggggtgcc tgtcttcccg gaccaaagac tacagaccac
      661 aggtgaaagt gaaaaacatg gatttaacac aacttacact caaagaggcg gtcgagttcg
      721 ccaaaaagga cggagccgga ccgattctcg acgctttcga gaagcgtgcg gcggaactga
      781 atcccgggat caacgcgttt ttaaagatgc cgtgtgcgat ggagacgggt tcgagaactt
      841 gtcccgaagg agtacccggt accttttatg gtgttccggt ctccgtcaag gacaatatct
      901 gtatcgaagg cgtagaagtg acctgtgcgt cgaagatcct cgaaaaacat atcccgcctt
      961 acgatgctac cgtgatcacg aagctcagga acgccggcgt gacgctttgg ggccggtgca
     1021 acatggacga atttgcgctc gggtcttcct gcgaaacctc cgcgttcggc ccgtgcaaaa
     1081 atccgtggga catcacccgt gtgccgggcg gctcaagcgg gggatcggct gcggcggttg
     1141 ccgccgatat gaccctggca gcgctcggct ccgataccgg cggctcaatc cgccagcccg
     1201 cggcgctctg cggagtggtc ggcgtgaaac cgacttacgg ccgcgtgtcg cgctacggtc
     1261 tcattgcgtg cagttccagc tttgaccaga tcggtccgct cgccaaaacc gtagaagatg
     1321 cggcgctcct tatgaacgtc atttcgggcc acgacccgaa agactctacg tcttctccgc
     1381 agcccgtgcc ggattttacg aaatcacttg tgcgggacgt aaaaggtctc cgcgtcggtc
     1441 ttcccaggga atatttcatc gaagggatcg acccggaagt cgagaaaagc gtgcgcgccg
     1501 cggtggacct ttacaggaag ctcggcgccc aaatccagga gatcagcctt ccgcacacgg
     1561 agtattccgt cgcggtctat tatattgtgg cggtcgcgga agcgagctcg aacctcagcc
     1621 gcttcgacgg tgtcca
//
LOCUS       DAOOBX010000228         1629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_82356_length_1629_cov_2.780178, whole genome shotgun sequence.
ACCESSION   DAOOBX010000228 DAOOBX010000000
VERSION     DAOOBX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82356_length_1629_cov_2.780178"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..836)
                     /locus_tag="PLY30_03700"
     CDS             complement(174..836)
                     /locus_tag="PLY30_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPW65245.1"
                     /translation="MKDKKTAAFFLTVLVLLAVPMPLLAGTREPLRLLLPQKDGGQPL
                     MKALAERKSTREFSGEELAPQVLSGLLWAAAGVNRPDGRRTVPSAHNAREIDIYVAKK
                     EGLYLYNAEQHALIPVLAKDIRPFAGKQDFVREAPVGLIFVADPGKFRGGSEEDAKFY
                     SAADTGFISQNVYLYCASEGLATVVLGWIDRERLAKEMGLRPEQKIILTQPVGYAKKN
                     DR"
     gene            complement(1018..>1629)
                     /locus_tag="PLY30_03705"
     CDS             complement(1018..>1629)
                     /locus_tag="PLY30_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW65246.1"
                     /translation="LGQIIRLRKKYGALLVVDDAHGTGVLGKEGRGATEGFSEGVDLV
                     MGTLSKTAGGLGGFVAADKVLVDHLINFARPFVFATALPPVLCAAAAEAFTVMEEEPG
                     IREKLRENIRKVHEGLTRLGFSLASPDSAILPVVLGDEGKALAAFEFLLSKGIYIPAV
                     RYPTVPKGKARLRVTVSAAHTATEIDALIDAFAALGERDGKNP"
BASE COUNT          332 a          510 c          447 g          340 t
ORIGIN      
        1 atagcagccc gctccctttt cgatgaccgg gaattcttcc acctgccaat cccgcatctg
       61 ggtgaacgga tgccagatat gttttcggtc gcttgagaca agctgtgctt tattcataaa
      121 tcaatcgggt tatttcccgc cggcgatcat gccggggatc gatcccgcat ggtttaccgg
      181 tcattttttt tcgcataccc caccggctgc gtcaggatga tcttctgctc cggccgaagc
      241 cccatctcct tcgcaagacg ctcccggtcg atccagccaa gaaccacggt cgcgagtccc
      301 tcggacgcgc aataaagata gacgttctgg ctgatgaatc ccgtgtccgc ggcgctgtag
      361 aatttcgcgt cctcttcgga accgccgcga aatttgccgg ggtccgcaac gaaaatcagc
      421 cctaccggcg cttcccggac aaaatcctgc ttcccggcaa acggccggat atctttcgcg
      481 agcaccggaa tcagcgcatg ttgttcggca ttatacaggt aaagtccttc ttttttggcg
      541 acataaatat cgatctcgcg ggcattatgc gctgagggta cagtccgtcg tccgtccggg
      601 cggttcacgc cggccgcggc ccagagaagg ccggaaagga cctgaggggc cagctcttcg
      661 ccgctgaatt cccgcgtgct cttccgctcc gcaagcgctt tcatcagggg ttgtccgccg
      721 tctttttgcg gcagcagaag ccggagaggc tcccgggtcc ccgccaaaag gggcatcggc
      781 accgccagga gtacgagaac cgttaaaaaa aacgccgctg tttttttgtc tttcatattc
      841 cctgcctttc cgcggggctt ccgccgagtc acttgtcgac aaaataatat tgcacacttc
      901 ccggaaggca cagcaaaata tacgaaagaa gccaccgggt catcgtaccg cctgcgggga
      961 tttttaacca ggaaagaaga accgccgcgg ggatcaggcg cggaatatcc ggcgcgatca
     1021 tggattcttt ccatcccgtt ctccgagggc ggcaaacgcg tcaatgagcg cgtcgatctc
     1081 tgtggccgtg tgagccgcgc tgaccgtgac gcgaagtcgg gcttttcctt tgggaaccgt
     1141 cggataccgg acagcgggga tgtaaatgcc ttttgagaga aggaactcaa aagcggcgag
     1201 cgccttcccc tcgtctccca ggaccacggg caggatcgcg gaatcgggcg aagccagcga
     1261 aaagccgagc cttgtcagtc cttcatgaac cttgcggata ttctcccgga gcttttcccg
     1321 gatcccgggc tcctcctcca tcactgtaaa agcttccgcg gcggcagcgc aaagaaccgg
     1381 cggcagtgcc gtcgcgaaaa caaagggccg cgcgaaattg atgagatgat cgaccaggac
     1441 cttgtccgcc gcgacaaagc ccccgagccc acccgccgtt tttgacagcg ttcccatgac
     1501 gaggtcgacg ccttccgaaa aaccttccgt cgccccgcgg ccttctttgc cgagaacgcc
     1561 cgtcccgtgc gcgtcgtcca cgacaagcaa ggcgccgtac tttttccgga gccggattat
     1621 ctgcccgag
//
LOCUS       DAOOBX010000229         1628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_82450_length_1628_cov_2.506039, whole genome shotgun sequence.
ACCESSION   DAOOBX010000229 DAOOBX010000000
VERSION     DAOOBX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82450_length_1628_cov_2.506039"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLY30_03710"
     CDS             <1..343
                     /locus_tag="PLY30_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPW65247.1"
                     /translation="VGGIWIMNIMLVSVKELTREIGLRKALGATSRDILVQFLVEAVM
                     ITFLGGLTGIVLAGVVSWLIATFAKWKMIITFGSLCLAFLFSAGVGILFGLWPAKQAA
                     ALDPIRSLRYE"
     gene            400..>1628
                     /locus_tag="PLY30_03715"
     CDS             400..>1628
                     /locus_tag="PLY30_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW65248.1"
                     /translation="MFRALRHRNYRLFFFGQGISLVGTWMQQVAVGWLVYRMTSSPIW
                     LGVVGFVGQIPTFLISPVAGVLADRHERRSLLIVTQVAALLQASLLAFLVLSQRIQVW
                     HVVALSAFLGIINSFDIPVRQSFTIEMVEEREDLGNAIALNSSMINAARLIGPSAAGL
                     LIAWAGEGVCFFLNAVSYIPVVLSLCAMKVVARRLFIPEQRIWHELREGLAYGFRSVP
                     IRWILFILSVVSLMGVAYPVLMPVFAKDVFRGGPHTLGFLMAMSGAGALAGALFLAAR
                     RSVVGLGKVLARMAGLFGISMIALSLSRSFSLSLLISWFSGFGMIVHTASCNTILQTI
                     VEEDKRGRVMSLYTMSFMGMAPFGSLLAGGLAGRIGAPLTIFFSGACCVAGAVVFLRK
                     LPIVKAKIRPIYAQKGI"
BASE COUNT          234 a          448 c          520 g          426 t
ORIGIN      
        1 ggtcggcggg atctggatca tgaatatcat gcttgtttcg gtgaaggagc tgacgcgtga
       61 gatcggtctt cggaaggcgc ttggtgccac gtcccgcgat atcctggtac agtttctcgt
      121 ggaagcggtt atgattactt ttctgggggg gctgaccggc atcgtcctgg ccggtgtggt
      181 ctcctggctg atcgcaacct tcgcgaagtg gaagatgatc attaccttcg gttccctctg
      241 cctcgcgttt cttttttccg cgggcgtggg aatcctgttc ggcctttggc cggcgaaaca
      301 ggctgctgcc cttgatccca tccgttccct gcggtacgaa tgagtccggc catgagcgtt
      361 ccggaccccc gaccttcccg cggaatcgac ttgcgatcca tgttccgcgc attgcggcac
      421 cgcaattacc ggctcttttt cttcgggcag ggaatctcgc tggtcgggac gtggatgcag
      481 caggtggccg tgggctggct ggtctaccgc atgacaagtt caccgatctg gctcggcgtt
      541 gtgggctttg tcgggcagat tccgaccttc ctgatctcgc cggtcgccgg cgtgctggcg
      601 gaccggcatg agcggcgatc ccttctgatc gtgacgcagg tcgcggccct tttgcaggcg
      661 tccctgctgg cctttcttgt gctctctcaa aggatacagg tgtggcatgt ggtcgcgctg
      721 agcgcttttc tgggcatcat caattctttc gatattcccg tccgtcaatc cttcacgatc
      781 gaaatggtcg aggagcgcga agatcttggt aatgcgatcg ccctgaactc gtcgatgata
      841 aacgcggcgc ggcttatcgg gccttcggcg gccggcctcc tgatcgcttg ggcgggggaa
      901 ggggtctgct ttttcctgaa cgcggtcagt tatattcccg tggtgctgtc gctttgcgcc
      961 atgaaggtcg tggccaggag gctgttcatt ccggagcaaa ggatctggca cgaactgagg
     1021 gaaggtcttg cctacgggtt ccgttccgtg ccgattcgct ggattctttt tatcctgtcg
     1081 gttgtgagtc tgatgggcgt tgcctatccg gttctgatgc cggtgttcgc gaaagatgtt
     1141 ttccgaggcg ggccgcatac cctcggtttc ctgatggcca tgtccggggc gggcgcgctg
     1201 gccggggcgc tttttctggc cgcgcgcagg agcgtggtcg ggctgggaaa ggttctcgcg
     1261 cggatggcgg gtcttttcgg gatcagcatg atcgcgctct ccttgtccag gagtttctcc
     1321 ctttcgcttc ttatcagctg gttctcgggt ttcgggatga tcgtccacac ggcttcctgt
     1381 aacactatcc ttcagaccat tgtggaagaa gacaagcggg gaagggtcat gagcctttac
     1441 acgatgtcgt tcatggggat ggcgcctttc gggagtctcc tggccggagg gctcgcgggc
     1501 aggatcggcg ctccccttac gatctttttc agcggcgcct gctgcgtcgc gggcgccgtt
     1561 gtttttttga ggaagcttcc cattgtcaag gcaaagatcc gccccatcta cgcgcagaag
     1621 gggatcat
//
LOCUS       DAOOBX010000230         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_82505_length_1627_cov_2.976463, whole genome shotgun sequence.
ACCESSION   DAOOBX010000230 DAOOBX010000000
VERSION     DAOOBX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82505_length_1627_cov_2.976463"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLY30_03720"
     CDS             <1..611
                     /locus_tag="PLY30_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65249.1"
                     /translation="PSDAKGGLVVPVSGLPEIGQIARDVFAMIVNTEVRKAYVAEGIS
                     ADTARSVLASFAHVRDLKGNPVLGRLEIVPAAKGDMIRAVTGAGAALAYRLGMKPCDV
                     LVLLEGARYAAIREKIAETALVSDLGERADLAPARDYLAMGAAMLNVRLDNDAWTLLN
                     QRVADALRKKDGFFEMDRNALAGLAAILTQIFAQERATATAA"
     gene            700..>1627
                     /locus_tag="PLY30_03725"
     CDS             700..>1627
                     /locus_tag="PLY30_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPW65250.1"
                     /translation="MRAEKRPLSIAIAQMNPTVGDCPGNAAKMLRWIREASAQGADLV
                     VFPECALCGYPVWDLANKKSFIEAGLRELKKIAAATRGLKTAVVLGFINAGPACGVRC
                     ATKRKSRDFALHTSFSFNAAAWLEAGKVRAIYHKRLLPTCDVFLEQIFFRAGNKPCVM
                     PFRGHRIGLTICEDIRDAAYDVKPLAELKRKGADLVINISASPYFEGVGAKREALVRA
                     HVRRYQVPMIYVNQVGGQDDLLFDGRSFCCDSRGRVTFQAPAFEERLLLAGEPIQPTR
                     LDSVGEMYQALVLGVRDYFRKNGFGKALIGLSG"
BASE COUNT          289 a          455 c          540 g          343 t
ORIGIN      
        1 cgccgtccga cgccaagggt ggtctggtcg tgcctgtttc gggtctcccg gagatcgggc
       61 agatcgcacg cgatgtgttc gcgatgatcg tgaacaccga ggtccggaag gcctatgtcg
      121 cggaagggat ttccgcggat acggcgcggt cggttctcgc gtcgttcgcg catgtgcgcg
      181 acctgaaggg caatcccgtg ctcgggcggc ttgagatcgt gccggccgcg aaaggggata
      241 tgattcgcgc ggtgacgggg gcgggcgccg cgctcgcata ccgtctcggg atgaaaccct
      301 gcgatgtgct cgtgctgctg gaaggcgcaa ggtacgccgc gatccgcgag aagatcgcgg
      361 agaccgcgct ggtgtctgac ctcggggagc gcgctgatct cgcgccggcc cgtgattatc
      421 tggcgatggg cgccgcgatg ctgaatgtcc gtctggataa cgacgcgtgg acgctgctca
      481 atcaaagggt cgcggatgcg ctccggaaga aggacggctt tttcgagatg gaccgcaacg
      541 ctctcgcggg tcttgccgcc atcctgacgc agatttttgc gcaggagaga gcgacggcga
      601 cagcggcata atttctgcgg acgcgccgcg gcgcgcggat tattgttttg cgctcttccg
      661 cgcctggcgg ccgcgacgct gtgatataat ccagattcta tgagagcaga aaagagacct
      721 ctgagcatcg cgatcgcgca aatgaacccc acggtcggag attgtccggg gaatgccgcg
      781 aagatgctcc gctggatccg ggaggcttcc gcgcagggcg ccgatctggt cgttttcccc
      841 gagtgtgcgc tttgcggata tcccgtctgg gacctcgcca acaagaaaag ttttatcgaa
      901 gccggtcttc gcgaattgaa gaagattgcg gcagccaccc ggggtctcaa gactgccgtt
      961 gtcctcggtt ttataaatgc cggtccggcg tgcggggtac ggtgcgcgac gaaaaggaaa
     1021 agccgtgact tcgcactcca tacttccttc tcattcaacg ccgcggcgtg gcttgaggcc
     1081 gggaaggttc gcgccattta ccacaagcgt cttttgccga cctgcgatgt ttttctcgaa
     1141 cagatctttt tccgggcggg gaataaaccg tgcgtgatgc cgttccgggg gcaccggatc
     1201 ggtctgacaa tttgcgagga tatccgggac gccgcgtatg atgtcaagcc gctcgcggaa
     1261 ctcaaacgaa agggcgccga tcttgtcatc aatatctcgg cctccccgta ttttgaaggc
     1321 gtgggcgcca aacgcgaggc gctcgtgcgc gctcatgtcc ggcgttatca ggtccccatg
     1381 atctatgtca atcaggtcgg cgggcaggat gacctccttt ttgacggccg gagtttttgt
     1441 tgcgactccc gggggcgtgt gacatttcag gcacccgctt tcgaagaacg cctcctcctg
     1501 gctggtgaac cgatccaacc tacgaggttg gattcggtgg gggagatgta ccaggcgctt
     1561 gtccttggcg tccgggacta tttccgcaaa aacgggttcg ggaaggccct gatcggattg
     1621 agcgggg
//
LOCUS       DAOOBX010000231         1598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_84553_length_1598_cov_2.778354, whole genome shotgun sequence.
ACCESSION   DAOOBX010000231 DAOOBX010000000
VERSION     DAOOBX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84553_length_1598_cov_2.778354"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..419)
                     /locus_tag="PLY30_03730"
     CDS             complement(207..419)
                     /locus_tag="PLY30_03730"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPW65251.1"
                     /translation="MQAQYYAIGETAGKIYRLLEQKGEQTVTKAQKEIGADDAIFYQA
                     LGWLARENNVNFNKLGKTLKISLLTR"
     gene            complement(476..>1598)
                     /locus_tag="PLY30_03735"
     CDS             complement(476..>1598)
                     /locus_tag="PLY30_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPW65252.1"
                     /translation="FLPLISRTESAVRGQIETAAKLYEEVLGRRPIGIWLPECGYYPG
                     VDKFLKEQGIRYFLTDTHGILHASPRPKYGVFAPIFCPTGVAAFGRDVESSKQVWSAV
                     EGYPGDLDYREFYRDIGFDLDYDYIRPYISPDGIRVNTGIKYYRITGDTDDKQPYVRA
                     WAMEKAASHAGNFMFNREKQVEYLHDFMGRKPLIIAPYDAELYGHWWYEGPDFLNFFI
                     RKSAFDQKVFRLIAPSDYFREYPVNQVSTPSFSSWGWKGYSETWLSGSNDWIYRHIHK
                     ATLRMSELAGRFRGQNGVVGRALKQAARELYLAQSSDWAFIMHTGTMVPYAVKRTKDH
                     ILNFTKIYEDLMNDRLDEAFLRDLEHKNNIFPNVDYNMF"
BASE COUNT          341 a          448 c          445 g          364 t
ORIGIN      
        1 gggaaacgaa gaaaaaagcg ttaatctcta tcagcagcgg atgttttttc atgttaaggc
       61 tctcggcagt agtttatcgg tttttccgcc gtttccttga tgcgggagac cgtacgccgc
      121 ccggaattca cggcggcatc cgcgaccaca aaaaaacggc cccggctccc gaaggagcaa
      181 gggccgtctg aaatctttca gggaccttac cgcgtcagga gcgaaatctt gagggtcttg
      241 cctaacttgt tgaaattgac gttgttctcg cgagccagcc aaccgagggc ctgataaaag
      301 atggcatcgt ccgcgccgat ctctttctgg gcttttgtca cggtctgttc gcccttttgt
      361 tcaagaaggc ggtaaatttt ccccgccgtt tcgccgatcg cgtaatattg agcctgcatg
      421 acggttcctt tctgtttttt atgatgctga aaactccccg gaaaaagcgg agagatcaga
      481 acatgttgta atcaacgttc gggaaaatat tgttcttgtg ttcgagatcc ctaaggaacg
      541 cctcgtccag ccggtcgttc atcaggtcct cgtagatctt cgtgaaatta aggatatgat
      601 ctttcgtgcg cttgacggca tacggcacca ttgtccccgt atgcatgatg aacgcccagt
      661 cgctcgattg cgcgaggtag agttcacgcg cggcctgttt cagggcgcgt ccgaccaccc
      721 cgttctgccc ccggaaacgt ccggcaagct cgctcatcct cagtgtcgcc ttgtggatgt
      781 gccggtagat ccagtcgttc gatcccgaca accatgtctc gctgtagccc ttccaccccc
      841 aactcgagaa cgaaggggtc gagacctggt tgacggggta ttccctgaaa taatccgacg
      901 gtgcgatcag acggaacacc ttttgatcga acgcgctctt gcggatgaaa aagttcagga
      961 agtccggacc ttcataccac cagtgaccgt aaagctcggc gtcatagggg gcgatgatca
     1021 ggggcttgcg gcccataaag tcgtgaaggt actcgacctg tttttcgcga ttgaacatga
     1081 agttgcccgc gtgagaagcg gccttttcca tcgcccaggc gcgaacatac ggctgcttgt
     1141 cgtccgtgtc gcccgtgatc cggtaatatt tgatgcccgt gttcacgcga ataccgtccg
     1201 gcgaaatgta gggacggatg taatcgtaat cgagatcaaa accgatgtcg cgatagaact
     1261 cccggtaatc cagatcgccc ggatacccct ccaccgccga ccacacctgt ttcgacgatt
     1321 ctacgtcgcg gccgaacgcg gcgacgccgg tcgggcagaa gatcggcgcg aaaacgccat
     1381 acttcggacg gggtgaggcg tgaaggatgc cgtgcgtgtc ggtcaggaaa taccggatcc
     1441 cctgttcttt caggaacttg tcgacgcccg gataataacc gcattcggga agccagatgc
     1501 cgatcggacg ccgcccgagg acctcttcgt agagtttcgc cgcggtctcg atctgcccgc
     1561 gcacggccga ctccgtgcgg ctgatgagcg ggaggaac
//
LOCUS       DAOOBX010000232         1586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_85463_length_1586_cov_2.484651, whole genome shotgun sequence.
ACCESSION   DAOOBX010000232 DAOOBX010000000
VERSION     DAOOBX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85463_length_1586_cov_2.484651"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1586
                     /locus_tag="PLY30_03740"
     CDS             <1..>1586
                     /locus_tag="PLY30_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65253.1"
                     /translation="RTFRDGEKWAFSEQVYVDITDVNDRAGQLGTPELRAQYRAALLD
                     VMDSTYYTRMTSGANVTYSWKSKDGVQTYTLSEERVVVTPAAEAEGGTPGITPPVYSD
                     SNAKWGFSAQFRVQKGEVASHVNDLESEYRNAYAAALRVPSEAYFRTVSGADTSYSWT
                     ALDKGVTYSLSCSPEKVYVPATEVVDGVVRIKTDAAGKVIYETTNGVFDTEILPKWIF
                     SEQVVVPKETVHANIALLETDAVRAQAYAALDGVDAVAAFTRTLSGTATSFSWESLTG
                     GKTFGLNCDATKVMTSEAPLPVYADGFKWTFNETSLALRSDVEAQASHVPADQRQQYD
                     VALADTDDHSAYFTRSLSGTAVNYSWRSLGGVKTYSLAIEQVKTGDVLTPAKWTMSVQ
                     TRVRKSEVEKQVKEFKNRSMRQQYLDALDLDDEGNPDETTNDVDDATVYTKTEIGSDV
                     SYSWKNLDNTATFSLNRSLQRISDAAGNLAGTRIQWTFSKQVLVTGDLVDDYAGKIEN
                     GAVRTQYADALNDVDMATEY"
BASE COUNT          366 a          417 c          494 g          309 t
ORIGIN      
        1 cgcggacatt cagggatggc gaaaaatggg cttttagcga gcaggtgtat gtcgatatca
       61 ctgacgtcaa tgatcgcgcc ggtcaattgg gcacaccgga actccgggcc cagtatcgcg
      121 ccgcgctttt ggatgttatg gacagtacct attacacgag gatgacgagc ggcgcgaacg
      181 tcacttacag ctggaaatcg aaagacggcg tccaaaccta cactctttcc gaagaacgcg
      241 tggtcgtgac gccggccgcc gaagcggaag gcggtacccc ggggatcacg cctcccgttt
      301 attcggattc aaacgcgaag tggggtttca gcgcccagtt ccgggttcaa aagggggagg
      361 tagcgagcca cgtgaacgat cttgaatcag aataccggaa cgcgtacgct gcggcgcttc
      421 gtgttccttc cgaagcctat ttcaggaccg tcagcggtgc ggataccagc tacagctgga
      481 cggctctgga taaaggcgtg acctactcgc tcagctgttc cccggagaag gtttatgtgc
      541 cggcaaccga ggtcgtggac ggggtcgtaa ggatcaagac ggacgcggcc ggcaaggtca
      601 tctatgagac gacgaacggc gtgtttgaca cggagatcct tcccaaatgg atctttagcg
      661 aacaggttgt tgttccgaag gagaccgtcc atgcgaatat cgcgctgctt gaaacggacg
      721 cggttcgcgc gcaggcgtat gccgcgctgg acggagtgga tgccgttgcg gctttcaccc
      781 gcaccttgag cgggaccgcc acgagcttta gctgggagtc cctgaccggc ggcaagactt
      841 tcgggcttaa ctgcgatgcg acgaaagtga tgacctctga agcaccgttg cccgtttacg
      901 cggacggttt caagtggaca ttcaacgaaa catcgctcgc tctccggtcg gatgtggagg
      961 cccaggcatc ccatgttccc gcggatcagc ggcagcagta tgatgtcgcc ctcgcggata
     1021 ccgatgatca ttcggcctat ttcacgagat cgctgagcgg gacggccgtg aactacagct
     1081 ggcgctcgct cggcggggtc aagacctact cgctcgcgat tgagcaggtg aagacgggtg
     1141 acgtcctgac gcccgcgaag tggacgatga gcgttcagac gcgtgtccgg aagagcgagg
     1201 ttgagaaaca ggtcaaggaa ttcaaaaacc gttcgatgcg gcaacagtat ctggacgcgc
     1261 ttgacctgga cgatgaagga aatccggacg aaaccacgaa cgatgtagat gacgcgacag
     1321 tctacacgaa gactgaaatc ggttcggatg tgtcctatag ctggaaaaac ctcgataaca
     1381 cggcgacatt cagtttgaac cggagccttc aaaggatatc ggatgctgcc ggtaacctgg
     1441 ccggaacccg gattcaatgg actttctcga aacaggttct ggtgaccgga gaccttgtgg
     1501 atgattacgc cggaaagatc gagaacggcg ctgtccggac ccagtatgca gatgccctga
     1561 acgatgtgga tatggccacg gagtac
//
LOCUS       DAOOBX010000233         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_85819_length_1581_cov_2.903014, whole genome shotgun sequence.
ACCESSION   DAOOBX010000233 DAOOBX010000000
VERSION     DAOOBX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85819_length_1581_cov_2.903014"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /gene="ispF"
                     /locus_tag="PLY30_03745"
     CDS             <1..1197
                     /gene="ispF"
                     /locus_tag="PLY30_03745"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPW65254.1"
                     /translation="PAAGLGTRFVDSLRKPGAPRPSDRSKIFYQLGGEPVLLRTLRVF
                     AGIPEIREIILAVSPEMHAGVRSLLRDLNDPRVTWVPGGKTRAQSVWNALRRTSSTSE
                     WVLVHDGARPLITERDIRKVLRAGNGWDGVILARKVVPTIKRVLKSDGHIEGTVDRSV
                     LYEAETPQMVRREVLLRAYKADSAAVLRATDEAGLLEAIGARVKIVTHTGWNPKLTTV
                     TDLMLAQAMMEKGGAPGVRIGIGFDTHRLVPGRKLWLGGVTVPSTKGSLGHSDGDALL
                     HAVADAILGGIGRGDIGEWFSDKDRRWKNMKSSIIVSTVMREAKKLGWVPYHIDSNII
                     LQTPKLGPHKMRIRKKVAKIVGLPPDSVSVKARTREGLGPEGEGLAVTCQAVVAMKRV
                     SDDQIP"
     gene            1181..>1581
                     /locus_tag="PLY30_03750"
     CDS             1181..>1581
                     /locus_tag="PLY30_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPW65255.1"
                     /translation="MIRFHNTLTGRLEEFTPLVPGRVSLYTCGPTVYHYAHIGNFRTF
                     VFEDLLRRYLESRGYQVTHVMNVTDVDDKTIAGAIREKKSLGEYTSFYTQAFLDDMKT
                     LHVLAPTIRPHATGEIPAMIRLIGDLEKKGIA"
BASE COUNT          331 a          423 c          502 g          325 t
ORIGIN      
        1 cccgccgcgg ggcttggaac gcgttttgtc gatagcttaa ggaagcccgg agccccgagg
       61 ccttccgatc gctccaagat cttttatcag ctcggcggtg aaccggtgct cctgcgaacc
      121 ctcagggtat tcgcgggaat accggaaatt cgcgagatta tccttgcggt gagtcccgaa
      181 atgcacgccg gcgtacggtc cctgttaagg gatctgaatg atccgcgcgt gacctgggtg
      241 cctggcggga agacccgtgc ccaatcggta tggaacgcgc tccggagaac atcctcaaca
      301 tccgagtggg tcctggtcca tgatggcgcg cgccctctca tcacggaacg cgatatccgg
      361 aaagtgctca gggcgggcaa cggatgggat ggtgttattc tggcgcgcaa ggtcgtcccg
      421 acaatcaagc gtgttttgaa aagcgacggc catatcgaag gtacggtgga ccggagcgtg
      481 ctttatgaag cggaaacccc tcagatggtc cgtcgtgagg tcctgcttcg ggcttataaa
      541 gcggattccg ccgcggtatt gcgcgcgacc gacgaagcgg gacttctgga ggcgattggc
      601 gcccgtgtga agatcgtgac ccataccggc tggaacccga aactgacaac tgttacggat
      661 cttatgctcg ctcaggcgat gatggaaaaa ggtggcgctc cgggcgtccg gatcgggatc
      721 ggctttgaca cgcaccggct tgtccccggg aggaaacttt ggcttggcgg cgtgaccgtc
      781 ccgtcgacga aaggttcttt ggggcattcg gacggggacg cgcttcttca cgccgtcgct
      841 gacgcgatcc ttggggggat cggccggggc gatatcggag aatggttttc ggacaaggat
      901 cgccgctgga agaacatgaa aagttccatt attgtcagta cggtcatgag ggaagcgaaa
      961 aagctcggct gggtcccgta tcacatcgat tcgaatatta tcctgcaaac gccaaagctt
     1021 ggccctcaca agatgaggat tcgaaaaaag gtcgcgaaga ttgtcgggtt gccgcccgat
     1081 agcgtttcgg tcaaggcgcg gacgcgggag ggactggggc ccgaaggtga aggcctcgcc
     1141 gtgacctgcc aggcggttgt cgcgatgaag agggtttcag atgatcagat tccataacac
     1201 gttgaccggc cggcttgagg agttcacgcc tcttgtcccg ggcagggttt cgctttatac
     1261 ctgcggtccg acggtctatc actacgcgca tatcggcaat ttcaggacct ttgttttcga
     1321 ggacctcctg cgccgatacc ttgaatcccg ggggtaccag gtcacgcatg tgatgaatgt
     1381 gaccgatgtt gacgacaaga ccatcgccgg tgcgatccgc gagaaaaaat cgctcgggga
     1441 atacacttcc ttttacacgc aggcattcct ggatgatatg aagacgctgc atgtcttggc
     1501 gccgacgatc cggccgcacg cgaccgggga gatccccgcc atgatcagac tgatcgggga
     1561 ccttgaaaag aaagggatcg c
//
LOCUS       DAOOBX010000234         1568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_86812_length_1568_cov_3.126900, whole genome shotgun sequence.
ACCESSION   DAOOBX010000234 DAOOBX010000000
VERSION     DAOOBX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86812_length_1568_cov_3.126900"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..725)
                     /locus_tag="PLY30_03755"
     CDS             complement(120..725)
                     /locus_tag="PLY30_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPW65256.1"
                     /translation="MTTKEMKLNTAGLTKLRELTGAGMMDCKAALVEAKGDIKAAQDI
                     IRKKGLDIAKKKSTREVREGQIVSYIHAGGKLGVLLEVDCETDFVAKNADFQAFCRDI
                     AMQIAAAHPLFVSAENIPADVLEKEKSIFLDQVQGKPEAVQQKILEGKLAKRYEEICL
                     LNQKFIKDDSKTVQNILTEIIARIGENIIIKRFVRFEVGGH"
     gene            complement(739..>1568)
                     /gene="rpsB"
                     /locus_tag="PLY30_03760"
     CDS             complement(739..>1568)
                     /gene="rpsB"
                     /locus_tag="PLY30_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPW65257.1"
                     /translation="EAGVHFGHQTQRWNPKMKKYIFGERNGIYVINLEITLGCLEKAL
                     RFIKQIAAEGKNILFVGTKKQAQEPLKAAAELCGMPYVNQRWLGGMLTNFETIRKSVK
                     KLEEIDRMEKDGSFQFITKKEVSMLLKERERLVKNLSGIREMRKLPAAIFVIDAELEK
                     IGIAEARKLGIPVVAVLDTNGDPDPIDYPLPGNDDAIKAIKLFCEAVANAVREGRLEF
                     EKNAREKEEAEAQAAAAESEEAAIEIDETLEEKLVAEVAVEEVKPKTKKVSPKKDIA"
BASE COUNT          262 a          458 c          441 g          407 t
ORIGIN      
        1 ccccggacaa tttcaggcag atgcgtttat aaacgggctt tttcattttt ttcgtcgagc
       61 gcggagcgca gggcatcggg gacttttccg ttcccgccac gccaggcgct atccgccggt
      121 taatgaccgc cgacctcgaa gcggacgaac cgcttgatga tgatgttctc gccgatcctg
      181 gcgatgatct cggtcagaat gttttgcacg gtcttcgaat catccttgat gaacttctgg
      241 ttcagcaggc agatctcctc gtatcgtttc gcgagtttcc cctcgaggat cttctgctgg
      301 acggcctcag gcttgccctg gacctgatcg aggaagatcg acttttcctt ttcgagcacg
      361 tccgcgggaa tgttttcggc gctcacgaag agcggatgcg cggccgcgat ctgcatcgcg
      421 atatcgcggc agaaggcctg gaaatccgcg ttcttcgcga caaagtcggt ctcgcaatcc
      481 acctcgagca ggaccccgag ctttcctccg gcgtggatgt aggaaacgat ctggccctcg
      541 cggacctcgc gggtggactt cttcttcgcg atatcgagcc ccttcttgcg aatgatgtcc
      601 tgggcggcct tgatgtcgcc tttggcttcc acaagcgcgg ccttgcagtc catcatgccc
      661 gcgcccgtca actcgcggag tttggtcaga cctgcggtat tgagcttcat ttctttagtc
      721 gtcatgggta agtatccttc aggcgatatc ctttttgggg gagacctttt tggtcttcgg
      781 tttcacttct tcgacggcca cttcagcgac gagtttctct tcgagggtct cgtcgatctc
      841 gatcgccgct tcttcggatt cggccgcggc ggcctgcgct tccgcctctt ccttttcgcg
      901 ggcgttcttc tcaaattcga ggcgcccttc gcggaccgca ttagcgaccg cttcgcagaa
      961 cagcttgatc gccttgatgg cgtcgtcatt accggggagc ggataatcga tcggatcggg
     1021 atcgccgttc gtgtcgagga ccgccacgac gggaatgccg agcttgcgtg cttccgcgat
     1081 gccgatcttt tcgagctccg cgtcgatcac aaagatcgcg gccgggagct tgcgcatttc
     1141 gcggatgccg gagaggtttt tgaccaggcg ttcgcgctcc ttcagcagca tggagacttc
     1201 ctttttcgtg atgaactgga aactgccgtc cttctccatc cggtcgatct cttcgagttt
     1261 cttgaccgat ttgcggatgg tttcgaagtt cgtgagcatg ccgccaagcc agcgctgatt
     1321 cacatagggc ataccgcaga gctccgcggc ggccttcagg ggctcctggg cctgtttctt
     1381 ggtgccgacg aagagaatgt tcttcccttc cgcggcaatc tgcttgatga accggagggc
     1441 tttctcgaga cagccgagag taatctcgag attaatgaca taaatgccgt tacgctcccc
     1501 gaaaatatac ttcttcatct tggggttcca gcgctgggtc tgatgcccga aatggactcc
     1561 ggcttcga
//
LOCUS       DAOOBX010000235         1562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_87327_length_1562_cov_2.626410, whole genome shotgun sequence.
ACCESSION   DAOOBX010000235 DAOOBX010000000
VERSION     DAOOBX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87327_length_1562_cov_2.626410"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="PLY30_03765"
     CDS             <1..1426
                     /locus_tag="PLY30_03765"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HPW65258.1"
                     /translation="WMYSGSTLSLVYVPKDRKTAYVMILGDSPVYIFKDRREPVPLPE
                     HGVAEDPDMRPAPGFEVAAEDGRVFIRNLRTGRSTTTTRGFGDRAFSPTLIRKPEILK
                     VDLTDARESIEILVATDGIHGDDPGGRVERDRRIRDLSLKGRMADDYVVLAGDHKALD
                     NVTVLRWVFEPETGDPRSEKRMAPAEDRGGLPGKRRDGSVLSKNVAGLPLSARRSEKR
                     SPAESQKRILDPNEIGVARAAAYVLRQERVLAIGRAELGRVLDSLVPAAHAMKIRTPG
                     SGTRAAKDLLMDPVKLESGREQVAVIFDAVPAANDPVLQTLVVLIANSKFLFQIIIPG
                     VSKTELGEFSRMLMKMAQKARKAGGRIDLSVDSDEMKIAGFFGRNSEGGLVYDLRGDE
                     GAGLRSLAPSLAKAGRGKRVLSVYNDKGMTLSQRSYGLIASLNTLLENKDLPRGVQPL
                     SAILLLAENIQIAAEAARSLASAA"
BASE COUNT          328 a          431 c          513 g          290 t
ORIGIN      
        1 gtggatgtat tcgggatcga cgctgtccct cgtctatgtc ccgaaggacc ggaaaaccgc
       61 atacgtcatg attctcggcg attcgccggt ctacattttc aaggaccgga gagagccggt
      121 gccgcttccc gaacatgggg tcgcggaaga cccggatatg cgccccgcgc ccggatttga
      181 ggttgcggcg gaggacgggc gcgttttcat ccggaatttg agaacgggga ggtccacgac
      241 cacgacgcgg ggttttggcg accgcgcttt ttcgcccacg ctgatccgga agcccgagat
      301 tctgaaggtc gatctgacgg acgcacggga gtctatcgag attcttgtag ccacggatgg
      361 catacacgga gatgacccgg gcggacgcgt ggagcgggac agaaggatac gggatttgtc
      421 tctcaagggg cgtatggccg atgactatgt ggtcctggcc ggcgatcaca aggcactgga
      481 taacgtcacc gtgctccgct gggtttttga accagagacc ggagatcccc gcagcgagaa
      541 aaggatggcc ccggccgaag acaggggggg gctgccgggg aaaagaaggg acggctccgt
      601 tctgtcgaag aacgtcgcgg ggcttcctct ttcagcccgg cggagcgaaa agcgttcccc
      661 ggcggagagc cagaaacgga tcctcgatcc aaacgaaatc ggcgtggcgc gagcggcggc
      721 gtatgtgctc cggcaggagc gagtactcgc gatcggacgg gcggaactcg ggcgagtcct
      781 cgattccctg gtgccagcgg cacacgcaat gaagatccgg accccgggtt ccgggactcg
      841 ggcagcaaaa gacctgctga tggatccggt gaagcttgag agcgggaggg agcaggtcgc
      901 ggtgatcttt gacgcggtcc ccgcggcgaa cgatcccgtc cttcaaacgc tcgtggtcct
      961 gatcgcgaac agcaaattcc tttttcagat catcatcccg ggtgtgtcga agaccgagct
     1021 cggggagttc agccggatgc tcatgaaaat ggcgcaaaaa gcgcgcaagg cgggcgggag
     1081 gatcgatctt tcggttgact ccgacgaaat gaaaattgcg ggattcttcg gacggaattc
     1141 cgaaggcggc ctggtctatg acctgcgcgg tgacgagggg gccgggttgc gctcgctcgc
     1201 gccgtcgctc gcgaaggcgg gccgcgggaa acgggtgctc tcggtctata acgacaaggg
     1261 gatgacgctg agccagaggt cctacggctt gatcgcttcc ctcaacacct tgcttgagaa
     1321 caaggacctc ccgcggggcg tgcagccgct gagtgccatt ctcctcctgg ccgagaatat
     1381 ccagatcgct gccgaagccg cccgctccct cgcctccgcc gcctaaccaa ttccaacctg
     1441 tgaggttgga tttcggtctc gaagcgcaag aaaaagtgga cggatccttt cgattattat
     1501 attataagca aacaaaatat tacttaaaac catatccaac ctcgtaggtt ggaggcggtg
     1561 ga
//
LOCUS       DAOOBX010000236         1556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_87771_length_1556_cov_3.445703, whole genome shotgun sequence.
ACCESSION   DAOOBX010000236 DAOOBX010000000
VERSION     DAOOBX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87771_length_1556_cov_3.445703"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PLY30_03770"
     CDS             <1..633
                     /locus_tag="PLY30_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113802327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPW65259.1"
                     /translation="DQKIQMIDLMEGEGAGIKSATFIAQGPYAYGYLKAESGVHRLVR
                     ISPFDANKRRHTSFASVDVIAEVEDTPEVEIKEADLRIDAYRAGGAGGQHVNKTSSAI
                     RITHIPTGIVVQCQNERSQGQNKAVAMKVLRARLYERYRQEQESELARKYGPKKKIEW
                     GSQIRSYVFHPYSMVKDHRTDVETSNVQAVMDGDLDEFIHVYLKQHVSKI"
     gene            688..1488
                     /locus_tag="PLY30_03775"
     CDS             688..1488
                     /locus_tag="PLY30_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HPW65260.1"
                     /translation="MKKFAQLFVLLVGCLVLSGCLTQVEDGQVGVKIDFGKIQEQPLG
                     AGWHFFVPAVTQIEIWNVKIQELKELAMVPSSEGLISQLDISILYQVPAGQASLVRRT
                     IGRYYVQIVLEPYVREAIRNVASGYAVKALYSEAGRKEIGKNMLDFLQSKLDEKGIIV
                     QDVLLRDVKLPPSFSQSIEAKLRAEQEALQKEFELQKAKKDAEIEVARAEGVAKANQI
                     IGDSLKNNEPYLRYLWINNLKETKNNVIYVPTEAGLPILEASRKTNTQ"
BASE COUNT          379 a          418 c          471 g          288 t
ORIGIN      
        1 gatcaaaaga tccagatgat cgatctgatg gaaggggaag gcgcggggat caagagcgcg
       61 acctttatcg cacaggggcc ctacgcgtac ggctacctca aggccgaatc cggcgtgcat
      121 cgccttgtcc gaatctcgcc gttcgacgcg aacaaacgac gccacacctc ctttgcctct
      181 gtcgatgtga tcgcggaggt tgaggacacg cccgaagtcg agatcaagga agcggatctc
      241 cggatcgacg cctatcgcgc gggaggcgcc ggtggccagc atgtcaacaa gacctcgagc
      301 gcgatccgca tcacgcacat tccgaccggt atcgtggtac aatgccagaa cgaacgttcc
      361 cagggacaga ataaagcggt cgcgatgaag gtcctccggg cgcgccttta cgagcggtac
      421 cggcaggagc aggaatccga acttgccagg aaatacggcc ccaagaaaaa gatcgagtgg
      481 ggttcccaga tccggtccta tgtgttccat ccgtattcca tggtgaaaga tcaccggacg
      541 gacgtcgaga cttcgaatgt ccaggccgtg atggacgggg acctcgatga attcattcac
      601 gtctatctga aacagcacgt gtcaaaaatc tgagaaccgg gactcgtccc cagggacgaa
      661 tcccgggtgg gttaaacgga ggcggtcatg aaaaagttcg cacaactatt tgtcttgctc
      721 gttggttgtt tggtcttgtc aggttgcctg acacaggttg aggacggcca ggtcggcgtg
      781 aagatcgatt tcggaaagat ccaggagcag ccgctcggcg cgggctggca cttcttcgtg
      841 ccggcggtga cacagatcga gatctggaac gtcaagatcc aggaactcaa ggagctggcc
      901 atggtgccgt cttcggaagg tctgatctct cagctcgata tctccatcct ttatcaggtc
      961 cccgcaggcc aggcgagcct tgtccgtcgc acgatcggac gctactatgt gcagatcgtg
     1021 ctcgagccgt atgtccgtga agcgatccgg aacgtcgcaa gcggctacgc ggtgaaggcc
     1081 ctctatagcg aggcgggccg gaaggagatc ggcaagaaca tgctcgattt cctgcaaagc
     1141 aagttggacg agaaaggcat catcgtgcag gatgtgcttc tgcgggatgt gaagctgccg
     1201 ccgtcgtttt cccagtcgat cgaggcgaag ctccgggccg aacaggaggc cctccagaag
     1261 gagttcgaat tgcagaaggc gaagaaagat gccgagatcg aggttgcgcg cgcggaaggt
     1321 gtcgcgaagg ccaaccagat cattggggat tccctcaaga acaacgaacc gtacctccgg
     1381 tacctctgga tcaacaacct caaggagacg aagaacaatg tgatctacgt cccgaccgag
     1441 gcggggctgc cgatcctcga ggcatccagg aaaacaaaca ctcaataaaa aggaatgggt
     1501 ccggggattc gttcccggga cccgtgaatt gctttaaaaa acatatccga aggaac
//
LOCUS       DAOOBX010000237         1555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_87834_length_1555_cov_4.078667, whole genome shotgun sequence.
ACCESSION   DAOOBX010000237 DAOOBX010000000
VERSION     DAOOBX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87834_length_1555_cov_4.078667"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLY30_03780"
     CDS             <1..319
                     /locus_tag="PLY30_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPW65261.1"
                     /translation="YALWGLQERGEIFVHPGDELYEGMIVGVNNKGHDLVVNAIREKK
                     QTNMRSSGSDEAIQLVPPREMTLEFALEFIEKDELVEVTPKNIRLRKRYLTDNDRRRQ
                     RRA"
     gene            605..880
                     /locus_tag="PLY30_03785"
     CDS             605..880
                     /locus_tag="PLY30_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65262.1"
                     /translation="MSMKKFVAALFVSMMMLGSPAAFAAVDCTRNSPMDEVGDWFATF
                     GKKDMEKTRILAERKAKRVAACTQREAAKAAKQAQKAAGDMKKKLGF"
     gene            997..>1555
                     /locus_tag="PLY30_03790"
     CDS             997..>1555
                     /locus_tag="PLY30_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPW65263.1"
                     /translation="MQDVGKGLIEAAGRGESGAFRELYDRTSGYVYTLAWRVTGRKQD
                     AEEVTQDVFVRLHRSLGSFKFESSFKTWLYRVTMNTALNHAKKRSRVSEREIGDEDGD
                     RAVAGPDALRAVEAGETELRLKAMLDRLNPDQRACIVLREIEGMDYQAIANALSVNLN
                     TVRSRLKRAREALMAMGKKEVTVHAM"
BASE COUNT          361 a          398 c          507 g          289 t
ORIGIN      
        1 gtacgccctc tgggggctcc aggagcgcgg cgagatattc gtgcatccgg gcgacgagct
       61 gtatgaaggc atgatcgtcg gcgtgaacaa taagggacat gatcttgtcg tgaacgcgat
      121 tcgcgagaag aaacagacga acatgcgctc atccggttcc gacgaggcga tccagctggt
      181 tccgccgagg gaaatgacgc tcgagttcgc gcttgagttc atcgaaaagg acgaactggt
      241 ggaggtcacg ccgaagaaca tccggttgcg gaagcgttat ctgaccgata acgaccgccg
      301 gcggcaacgg cgcgcctgaa gttccgtttt cattgcccga accggttttt ccattataat
      361 gacgccattc actcaaacgt ttttagaaag gaattcgcat gtcattagcc ggaagaaaac
      421 gcagacgtca aaccgagatc gtccaacgca tgaaacgccg cgccaaggtc gcgaagctca
      481 aggccaggat cgccgatgcc aagacggaag gcgacaaggt catcctgctc cgcaaactcc
      541 gtcgggtcgc tcccggatcg gtttcgtaat aaatttaaac gcagcgcacc ctcacagaaa
      601 ggatatgagc atgaagaagt ttgtggcagc tctttttgtt tcgatgatga tgcttggcag
      661 tcccgccgct tttgccgcgg tggattgcac caggaactcg cccatggacg aagtcggtga
      721 ctggtttgcc acgttcggga aaaaggatat ggaaaagacc cggatccttg ccgaacggaa
      781 agcgaagcgc gtcgcggcct gcacgcagcg cgaagcggcg aaagcggcca aacaagccca
      841 gaaggccgcg ggggatatga agaaaaagct cgggttctaa accgtaagta tatgcgacgg
      901 catgaaaggc cggggttcac gccccggcct tttttgtttc cgcggaactt ccggacgggg
      961 atggttgtct aacgtttcag aacaggggac cggtccatgc aggatgtcgg aaagggattg
     1021 atcgaggcgg cgggcagggg ggaatcggga gcgttccggg aactttatga ccggacctcc
     1081 ggttacgtct acacccttgc ctggcgggtt acaggccgca agcaggacgc ggaggaggtc
     1141 acgcaggatg ttttcgtgag gctccaccgg agcctcggtt ccttcaagtt tgaatcgtcg
     1201 ttcaagacgt ggctttaccg ggtgacgatg aacacggcct tgaaccacgc gaagaaaagg
     1261 agccgggtct cggagcggga gatcggggat gaggacggtg accgcgcggt cgcgggtccc
     1321 gacgccctcc gggccgtgga agcgggggaa acggagttga ggctgaaggc gatgctcgac
     1381 cgtctgaatc ccgaccagcg tgcctgtatc gtgctccgcg agatcgaagg aatggattat
     1441 caggcgatcg cgaacgcgct ttcggtcaat ctgaacacgg tgcggtcgcg gcttaaacgg
     1501 gcccgggagg ccctgatggc gatgggaaaa aaggaggtga cggtccatgc gatgt
//
LOCUS       DAOOBX010000238         1551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_88121_length_1551_cov_4.395053, whole genome shotgun sequence.
ACCESSION   DAOOBX010000238 DAOOBX010000000
VERSION     DAOOBX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88121_length_1551_cov_4.395053"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /gene="radC"
                     /locus_tag="PLY30_03795"
     CDS             <1..356
                     /gene="radC"
                     /locus_tag="PLY30_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPW65264.1"
                     /translation="AVTDYLTASLRDLKREVFKVIFLDKGLKILGEKDMFFGTVDEAA
                     VHPREVVREALFQNASSLVLVHNHPSGKVEPSREDYEITQKIKTACQTVSIRILDHII
                     IGENQYFSFSERSQL"
     gene            368..538
                     /locus_tag="PLY30_03800"
     CDS             368..538
                     /locus_tag="PLY30_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65265.1"
                     /translation="MRKILKEKTHRKLLKMHEALGKEIRRRSKLCTGRTLALLSDMHE
                     LVIRWDAWEDRK"
     gene            633..>1551
                     /locus_tag="PLY30_03805"
     CDS             633..>1551
                     /locus_tag="PLY30_03805"
                     /inference="COORDINATES: protein motif:HMM:NF023645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HPW65266.1"
                     /translation="MTTHVQVPEEAGVSLKKVCTHCGKEEPETGFLEHAGKLLCGDCF
                     EERVASCDHCKERFLKSAMTEVNDGIYCEKCIDEHFRYCSGCEAYIRADLSNYSELHD
                     RDFCESCFNERVTHCSACQDQIDPGSCFRDEENEPFCDDCWNNREEERLPRYQEFSGG
                     SFTECRSPRKYGIEIEAMMEEDADHLPSERLENWQQVTDGSLGDHGREYVSPILQGDE
                     GFQAIRKFTGGLQGWGYFVNRDCGLHVHIDGRDLGCMEIKKLLKITRYFEPVLYAMLP
                     ESRHEGSYSVPLERFPKSRFRIGAKDEEAL"
BASE COUNT          366 a          375 c          494 g          316 t
ORIGIN      
        1 aggcggtcac cgattacctc acggccagtc tccgggacct caagcgcgag gtctttaagg
       61 tgatcttcct cgacaagggc ttaaagatcc ttggggagaa ggacatgttc ttcggaacgg
      121 tggatgaggc ggctgtccat ccccgcgaag ttgtgcggga agcgctgttt caaaacgcgt
      181 cgagtcttgt gcttgtccat aaccacccat ccgggaaggt cgaacccagc cgcgaggatt
      241 acgagattac ccagaaaatc aagaccgcgt gccagacggt ctcgatccgg attttggatc
      301 acatcatcat cggcgagaac cagtacttca gtttctcgga acgaagccaa ctttaggcgg
      361 agggaccatg agaaaaattt tgaaggaaaa aactcacaga aaacttctca aaatgcacga
      421 ggcgcttggg aaggagatcc gccggagaag caagctttgc acgggaagga cgctcgcgct
      481 tttgtcggac atgcacgagc ttgtgatccg gtgggatgcg tgggaggaca ggaaataaac
      541 gttagggcgg cagggatcta agaaaaataa acggggcggt gttcctttcg ggggatgccg
      601 cttttttttc actcaaaagg aggaggtttc acatgacgac ccatgttcag gtgccggagg
      661 aagcgggagt gtctctgaag aaagtttgca cacactgcgg caaagaggaa ccggagaccg
      721 ggttccttga gcacgcgggg aagcttttgt gcggggattg ttttgaggag cgggtggcgt
      781 cctgcgatca ctgcaaagag aggtttctca agagcgccat gacggaagta aatgacggga
      841 tctattgcga gaagtgtatc gacgagcact tccgctattg ttcgggctgc gaggcctata
      901 tcagggcgga cctctcgaac tattccgagc tgcacgacag ggatttttgc gagagctgct
      961 ttaacgagcg cgtgacccat tgcagcgcgt gtcaggacca gatcgatccc ggttcctgtt
     1021 tccgggacga agagaacgag ccgttctgcg atgactgctg gaacaaccgt gaagaggaaa
     1081 gactgccgcg gtaccaggag ttttcaggcg gcagttttac cgagtgccgg tccccgcgga
     1141 aatacgggat cgagatcgag gccatgatgg aagaagacgc ggatcacttg ccgtctgaga
     1201 ggctcgagaa ctggcagcag gtgacggacg gatcccttgg ggatcacggg cgcgagtatg
     1261 tgagcccgat ccttcagggc gacgaaggat ttcaggcgat ccggaaattc accggcggtc
     1321 ttcagggctg ggggtatttc gtgaaccgcg actgcgggct tcacgtgcat atcgacggcc
     1381 gcgatctcgg ctgcatggag atcaaaaaac tcctcaagat cactcggtat ttcgagccgg
     1441 tgctttacgc catgctgccc gagtcccgcc atgaggggag ctattccgtg cctcttgaga
     1501 ggttcccgaa atcgaggttc cggatcggcg cgaaggacga ggaggcgctt a
//
LOCUS       DAOOBX010000239         1547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_88449_length_1547_cov_2.862601, whole genome shotgun sequence.
ACCESSION   DAOOBX010000239 DAOOBX010000000
VERSION     DAOOBX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88449_length_1547_cov_2.862601"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLY30_03810"
     CDS             <1..473
                     /locus_tag="PLY30_03810"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPW65267.1"
                     /translation="QDLALDAILKGKVVDGDVVVIRYEGPKGGPGMMEMLSPTSAIKG
                     RGIRAALITDGRFSGGTRGLCIGHVSPEAAAGGPIAVIRNGDVIEIDAVKRTLELLVP
                     AGEIKSRLRKLKPFSPRVTGGWLGRYSRHVLSAHTGAVMDNNVLFENSKKKKGS"
     gene            476..964
                     /locus_tag="PLY30_03815"
     CDS             476..964
                     /locus_tag="PLY30_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPW65268.1"
                     /translation="MISGKITKAINDQINKEIYSAYLYIGMAAYAASMALGGISNWFR
                     AQAKEELGHAEKFMNYLSEQGGELVLKAIAAPDQKFTSVAGLFDKTLDHEKEVTASIH
                     NLVTLAKKEADYATEIFLQWFVTEQIEEEASVSDIIQKLEIGGKGGNAILVLDKALAL
                     RK"
     gene            983..>1547
                     /locus_tag="PLY30_03820"
     CDS             983..>1547
                     /locus_tag="PLY30_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPW65269.1"
                     /translation="MKAIPVAENIWWVGAIDWKARYFHGHTYTTKRGTTYNAYLILDE
                     KITLIDAVYGPFTDEMVQRIKSVIPAEKIDTIIANHIEPDHSGAYPELLKLCPKAKFY
                     GSARAGQGLERYYHRKLDFTPVKTGDKLKIGKRTLRFIEIPMIHWPDSMMTYLEEDGV
                     LFSNDAFGQHYATNERFDDEVPRETLME"
BASE COUNT          383 a          397 c          460 g          307 t
ORIGIN      
        1 cccaggatct cgcgctcgac gcgatcctga aaggtaaagt ggtcgacggc gacgtggtcg
       61 tgatccgcta cgaaggtccg aaaggcggtc ccggcatgat ggaaatgctt tctccgacgt
      121 ccgcgatcaa gggacgcggg attcgcgccg cgcttatcac ggacggccgg ttttcgggcg
      181 gtacgcgcgg actctgcatc ggccacgttt caccggaagc ggcggcgggc ggtccgatcg
      241 ccgtgattcg gaacggggat gtgatcgaga tcgacgccgt gaaacggacg ctggaacttc
      301 ttgtccccgc cggggagatc aagtcccgcc tccggaagct gaaaccgttc agccccaggg
      361 tcactggcgg atggctcggg cgatattcgc gtcacgtgtt gagcgcccat accggggctg
      421 tgatggataa caacgtcctg ttcgaaaact ctaaaaagaa gaaagggagc taatcatgat
      481 cagcgggaaa ataacgaagg cgatcaacga ccagattaac aaggaaatct actcggctta
      541 tctttatatt ggaatggccg cttatgccgc ttccatggcc cttggcggga tctccaactg
      601 gtttcgcgcc caggcgaaag aagagctcgg ccacgccgag aagttcatga actatctgag
      661 cgagcagggc ggcgaacttg tcctcaaggc gatcgcggct ccggaccaga agttcacttc
      721 ggtcgcgggc ctttttgaca agacactcga tcatgaaaag gaagtgaccg cttcgatcca
      781 caatcttgtg acgctcgcga aaaaggaagc tgactacgcg acggagattt ttctgcagtg
      841 gttcgtgacc gagcagattg aagaggaggc gagcgtttcc gatattatac agaagcttga
      901 gatcggcggg aaaggcggta acgcaatcct tgttctcgac aaggcgttgg ctctccgcaa
      961 ataaacggaa aagaaatttc gcatgaaggc gataccggta gcggaaaaca tctggtgggt
     1021 cggggcgatt gactggaagg cccgttattt tcacggccac acctacacga ccaagcgcgg
     1081 cacgacctac aatgcctacc tgatcctcga tgaaaagatc acactgatcg acgcggttta
     1141 cgggcctttc acggacgaga tggtgcagcg gatcaaaagt gttatcccgg cagagaaaat
     1201 cgatacgatc attgccaacc acattgaacc ggaccattcg ggcgcttatc ccgagcttct
     1261 gaaactttgt ccgaaagcaa agttttacgg gagcgcgaga gcggggcagg ggcttgaacg
     1321 ctactatcac cggaaactgg attttactcc cgtgaagacc ggagataaac tgaagatcgg
     1381 aaagcgcacg ctccgtttta tcgaaatccc gatgatccat tggccggaca gcatgatgac
     1441 ttatctggaa gaggacggag ttcttttttc aaacgacgct ttcggacagc actatgcgac
     1501 gaacgagcgg tttgacgacg aggtgccccg ggaaacgctg atggagg
//
LOCUS       DAOOBX010000240         1546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_88492_length_1546_cov_4.906774, whole genome shotgun sequence.
ACCESSION   DAOOBX010000240 DAOOBX010000000
VERSION     DAOOBX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88492_length_1546_cov_4.906774"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1546
                     /locus_tag="PLY30_03825"
     CDS             <1..>1546
                     /locus_tag="PLY30_03825"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW65270.1"
                     /translation="TRTEVLRIDPPKKQVITKDLATGTERNEPYDFLILSPGAEPVRP
                     PLSGIDSKQIFTLRNLADMDAIKKAAANAKERALVIGGGYIGLEMAEALRGRQIPVTL
                     VEMAPQVMGPADPEMAAPLHQELLLHGVDLRLGTSVKAFRDEKDVLYADLSTGESLPF
                     DFAVLAIGVKPETRLAKDAGLKLGTRGGILVDPGMRTSDPSIFAVGDAIEIKDFITDE
                     SSLIPLAGPANRQGRIAADNIFGRNSVYKKTQGTAICKVFGLAIGMTGASEKTLKRLK
                     TPYEKVHIHAASHASYYPGAAKIDLKLIFDPKSGRILGAQAVGANGVDKRIDVIATAI
                     RAGLTIYDLEDMELSYAPPFGSAKDPVNYAGFVAANVLRGDLKIFHPEDLKNLKKDQQ
                     VLDVRPKETTVTGMIPGSINIPLEDLRERLNELSADKEYLVYCKVGLRGYLSCRILAQ
                     NGFRCRNLTGGYETYQASLGIVSRRPITQEMKSDTNEREPSMDKNEPPGTTREIDACG
                     LQCPGPI"
BASE COUNT          370 a          485 c          423 g          268 t
ORIGIN      
        1 cactcgcact gaagttttac ggatcgaccc ccccaaaaag caggtcatca cgaaagatct
       61 cgccacgggg accgaacgga acgagcctta tgacttcctc atcctgagcc ccggcgccga
      121 acccgtccgc cccccgctct ccggcatcga ttcgaaacaa atcttcacgc tccggaacct
      181 cgcggacatg gacgccatca aaaaagcggc ggcaaacgcg aaggaacgcg cgctcgttat
      241 cggaggcggc tacatcggac ttgaaatggc ggaagcgtta cgcgggcgac aaatccccgt
      301 cacgctcgtg gagatggccc cgcaggtcat gggccccgcg gatccggaaa tggcggcacc
      361 cctgcatcag gagcttttac ttcacggcgt cgatcttcgt ctggggactt cggttaaggc
      421 cttccgggac gaaaaagacg ttctttacgc cgacctcagc actggcgaat ccctgccctt
      481 cgactttgcc gtcctcgcaa tcggggtcaa gcccgaaaca agactcgcaa aagacgccgg
      541 cctcaaactc gggacccgcg gagggatcct ggtcgacccc gggatgcgga cgagcgatcc
      601 ctcgatcttc gccgtcggcg acgcgatcga aatcaaggat ttcatcacgg acgagtcttc
      661 gctcatcccc ctcgctggac ccgcaaaccg ccaggggcgg atcgctgcgg ataatatttt
      721 cggccgcaat tcagtttaca aaaaaacaca gggcaccgcg atctgcaaag tcttcggcct
      781 cgcgatcggc atgacgggtg cgagtgaaaa gacgctgaaa cgactgaaaa ccccttacga
      841 gaaggttcat atccacgccg cctctcacgc gagctactat cccggcgcgg cgaagatcga
      901 tctcaagctg atcttcgatc cgaagagcgg aaggattctc ggcgcgcagg ccgtcggagc
      961 aaacggggtg gacaaacgga tcgacgtaat cgctacggcc atccgcgcgg gcctcaccat
     1021 ctacgatctc gaagatatgg agctttccta cgctcccccg ttcggatccg ccaaggatcc
     1081 tgtcaattac gcgggattcg tcgccgcgaa cgtcctcagg ggcgacctca aaatcttcca
     1141 tccggaagac ctcaaaaatc ttaaaaaaga tcagcaggtc ctcgacgttc gtccgaagga
     1201 aacgaccgtc acgggcatga tccccggctc gatcaatatc ccgctggaag atctgaggga
     1261 acgtctgaac gagctgtccg cggacaaaga atatctggtt tattgcaagg tgggtctgcg
     1321 tggctatctt tcctgccgaa tcctcgccca gaacggcttc agatgccgga acctgacggg
     1381 aggatacgaa acttatcagg cgagcctcgg gatcgtttcc aggcgaccca tcacgcagga
     1441 aatgaaaagc gatacgaacg aaagggagcc ttccatggac aaaaacgaac cgccgggcac
     1501 caccagggag atcgacgcct gcggactcca gtgccccgga ccgatc
//
LOCUS       DAOOBX010000241         1542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_88814_length_1542_cov_3.663080, whole genome shotgun sequence.
ACCESSION   DAOOBX010000241 DAOOBX010000000
VERSION     DAOOBX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88814_length_1542_cov_3.663080"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>969
                     /locus_tag="PLY30_03830"
     CDS             <1..>969
                     /locus_tag="PLY30_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65271.1"
                     /translation="RAASIKVGKRWKFIGVKNGRTWEDREGAQAIISTNFRPGSSSPF
                     AVAGFTALTEKWDKGSSIHELTHFNSLMRQRLKELGKDTDSGIGLEEVFNFLIQYRSG
                     LAEAGRGVYNIERVLDEKMTQYGHLFGEGLEGKRNAKLAFMTVLYMEEIGLSRWEIMN
                     ILGRITTVQDLIQFQLYTAEDIAKIRGSVALGEKKARAEAADKEAEDLENRVKVAQAR
                     LDDARKTFEAQWADNAGKREASAAFRKARELKEAAETERSNTAKAARRARAKASTADR
                     ELEAAQRKHNRILSAPVPDGVKTARQGTGDLIPNMETDLTWPRISRL"
     assembly_gap    970..979
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <980..>1542
                     /locus_tag="PLY30_03835"
     CDS             <980..>1542
                     /locus_tag="PLY30_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65272.1"
                     /translation="VLARILDASPKERLWMFYELTKRADSREDDGEGVTAAARAKAKS
                     VLAAFYADKDAQAVLIGLVRVARSVLHGQRDKDRLESLLLTPVLISAPEYLQTKVTEY
                     TGWLPVAQGVLKTTAEGRQVAEESYYAAKTQELVDARNWRELEEIVRGKRGNEETRAW
                     TVYYLYLHYRYDANVRRVLSYFESVRG"
BASE COUNT          344 a          408 c          521 g          259 t
ORIGIN      
        1 agggccgcgt cgatcaaggt cgggaaacgc tggaagttta tcggtgtgaa gaacggaaga
       61 acatgggagg accgggaagg tgcccaggcc atcatttcga ccaatttcag gcccggaagc
      121 tcgtcgcctt ttgcggttgc cggtttcacc gcgctgaccg aaaaatggga caaggggtct
      181 tcgatccatg agctgaccca cttcaacagc ctgatgcgtc agcggttgaa agaactcgga
      241 aaagacacgg attccggcat cggactcgaa gaggtgttca acttcctgat ccagtaccgg
      301 agcggtttgg ccgaggcggg acgcggtgtt tacaatatcg agcgggtgct ggatgaaaag
      361 atgacgcagt acgggcatct tttcggagaa ggtctggaag ggaagcggaa cgcgaaactt
      421 gcttttatga cagtgcttta catggaagag atcggcctga gccgctggga gatcatgaac
      481 atcctcggaa ggatcacgac ggtgcaggac ctgatccagt ttcagctgta tacggcggag
      541 gacatcgcga agatccgcgg gtcggttgcg ctcggcgaga agaaggcccg ggccgaggct
      601 gctgataagg aagcggaaga tctcgaaaac agggtgaagg tggcccaggc gaggcttgat
      661 gacgccagga agactttcga ggctcaatgg gcggataatg ccggcaagcg ggaagccagc
      721 gccgcgttca ggaaggcacg cgagctcaag gaagccgccg agacggagcg tagtaatacc
      781 gcgaaagccg cccggagagc gcgcgccaaa gcgtccacgg cggaccggga actcgaggcc
      841 gcgcagcgta agcataaccg catcctttcc gcgcccgttc ctgatggagt caagacggcg
      901 cgccagggga ccggcgatct gatcccgaac atggagacgg acctgacatg gcccaggatc
      961 tcccggcttn nnnnnnnnna tgtgctggcc aggatccttg atgcgtctcc caaagaacgg
     1021 ctttggatgt tctatgagct tacgaaacgc gcggacagcc gcgaggatga cggtgagggt
     1081 gtgacggccg cggcgcgcgc gaaagccaaa tcggttctgg ccgctttcta tgccgataag
     1141 gacgcgcagg cggtcctgat cggtctcgtc cgggtcgccc ggtccgtgct gcatgggcag
     1201 cgggacaagg atcgcctcga aagcctgctg ctgacgccgg tcctcatttc ggcgccggag
     1261 tacctgcaga cgaaggtgac ggaatacacc ggatggctgc cggtggcaca aggggtcctc
     1321 aagaccaccg ccgaagggcg tcaggtggcc gaagagtcct attacgcggc gaagacgcag
     1381 gagcttgtgg acgcccgcaa ctggcgcgaa ctcgaggaga tcgttcgcgg gaagcgcggc
     1441 aacgaagaga cgcgggcgtg gacggtctat tatctgtacc tccattatcg ctacgacgcg
     1501 aacgtcaggc gcgtcctcag ctattttgaa agcgtccgcg gc
//
LOCUS       DAOOBX010000242         1536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_89293_length_1536_cov_3.283592, whole genome shotgun sequence.
ACCESSION   DAOOBX010000242 DAOOBX010000000
VERSION     DAOOBX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89293_length_1536_cov_3.283592"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PLY30_03840"
     CDS             <1..414
                     /locus_tag="PLY30_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPW65273.1"
                     /translation="HALVEFVDGSHMAHLGVTDMRIPIQYAMSYPGRLENHLPHLDLA
                     RLRTLHFEKPDMKRFPCLALGYEASRIGGTMPAVLNAANEVAVEAFLGGKAGFMDIPK
                     VIERTMMRHSLVLKPGLEEILDADLRARQTASELL"
     gene            411..1535
                     /gene="rseP"
                     /locus_tag="PLY30_03845"
     CDS             411..1535
                     /gene="rseP"
                     /locus_tag="PLY30_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPW65274.1"
                     /translation="MSHLLSVVWNILPALVVFGILIIVHEWGHFIACRMCGVKVEKFS
                     IGFGPELFGWNSKETRFVVSLLPLGGFIKPSGEEASAVGAEGPKPGDFLAATAWSRII
                     IVTAGVTMNFALAFVLFSAVFMTGRPMPGTVIGGFIKGYPGESSGLAVKDRIVRVNGT
                     VTGNWKDVLAALDSATGPVIEIAVVRTAGQELSVGVIPKVEATRDIFGKPVTLRRIGI
                     TPDPEIVVTEKYGVLESIAMGWETVVSQTVLTYKAIYYICARRLSPKNLMGPLGIVQV
                     SGQAARAGIPALVQLMAILSVSLAAINLLPIPALDGGHLVFLLIEALFRRPIPVKVQE
                     KVTNAGFYALMGLMVLVFYNDIVNLQVVDKIRNLLHLSGH"
BASE COUNT          280 a          421 c          491 g          344 t
ORIGIN      
        1 cacgccctgg tcgaattcgt tgacgggtcg cacatggcgc atcttggcgt gacggacatg
       61 cggattccga tccagtacgc gatgagctat ccggggcggc tggagaacca tcttccgcac
      121 ctggacctcg cgcggttgcg gaccctgcat tttgaaaagc cggacatgaa acgttttccg
      181 tgtctcgcgc tcggatacga ggcgagcagg atcggcggca cgatgccggc cgtcctgaac
      241 gccgccaacg aagtcgcggt cgaggctttc cttggcggga aggccggttt tatggatatc
      301 ccgaaggtca ttgaacgaac catgatgcgt cattcgcttg ttcttaaacc cgggctggag
      361 gagattctcg atgcggatct ccgggcccgc caaacagcat cggaactttt atgagccatc
      421 ttctttccgt agtctggaac atccttcccg cgctggtcgt gttcgggatt ctgattatcg
      481 ttcatgaatg gggccatttc atcgcctgtc gcatgtgcgg cgtgaaagtg gaaaagtttt
      541 ccatcggatt cggaccggaa ctgttcggat ggaactcgaa agagacccgc tttgtcgttt
      601 ccctgctccc gctcggtgga ttcatcaagc cgtccgggga agaggcttcg gcggtggggg
      661 cggaaggccc gaagcccggt gattttctcg ccgcgacggc ctggtcgcga atcatcatcg
      721 tgacggcggg cgtgaccatg aacttcgcgc tggcgttcgt gctcttttcg gccgttttta
      781 tgacgggacg tccgatgccg gggacggtga tcggcgggtt catcaaggga tatcccgggg
      841 aatcgagcgg tctcgcggtg aaagacagga tcgtgcgcgt gaacgggacc gtgaccggga
      901 actggaagga cgttctcgcc gcgcttgatt cggcgacggg tcccgtgatc gagatcgccg
      961 tcgtgcgcac cgcagggcag gagctttcgg tgggcgtgat ccccaaagtc gaggcgaccc
     1021 gcgatatctt cggtaaaccc gtcacgctgc gccggatcgg gatcacgccg gatccggaga
     1081 tcgtggtgac cgaaaaatac ggtgttctcg aatcgatcgc tatgggatgg gagacggtcg
     1141 tttcccagac cgtgctgacc tataaggcca tctactacat ctgcgcgcgg agactttctc
     1201 cgaagaatct catggggccg ctcggtatcg tccaggtaag cgggcaggcg gcgcgggcgg
     1261 gaatcccggc gcttgtgcag ttgatggcga tcctgagcgt ttcgctcgcg gcgatcaacc
     1321 tcctgccgat ccccgccctg gacgggggac atcttgtctt tctcctgatc gaggccctct
     1381 ttcgccgccc gattcccgtg aaggtgcagg agaaagtgac gaatgccggc ttttacgcgc
     1441 tgatgggact gatggtgctg gttttctata acgatatcgt gaatctccag gttgtcgaca
     1501 agatcaggaa tctgctgcat ttgagcggtc attaaa
//
LOCUS       DAOOBX010000243         1530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_89758_length_1530_cov_3.242034, whole genome shotgun sequence.
ACCESSION   DAOOBX010000243 DAOOBX010000000
VERSION     DAOOBX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89758_length_1530_cov_3.242034"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..770
                     /gene="rplD"
                     /locus_tag="PLY30_03850"
     CDS             132..770
                     /gene="rplD"
                     /locus_tag="PLY30_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPW65275.1"
                     /translation="MKVPLYTKEGKKKQEVVLNPDIFGVRINARLIELVRNAYSANLR
                     RGTASTKTRGEVRGGGKKPWKQKGTGNARHGSNRSPIWVGGGVVFGPRPRDYSVRLSK
                     AIRTKALQVAIAMRAREKNVLLLETLKLETPKTKEFAGIVKTLPLGSKRAVYVTKEQD
                     VNMKRASRNLQKWVEVKNVADVSAYDVLQSPKVIIDEAALETLEKRLTGDSK"
     gene            770..1057
                     /gene="rplW"
                     /locus_tag="PLY30_03855"
     CDS             770..1057
                     /gene="rplW"
                     /locus_tag="PLY30_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPW65276.1"
                     /translation="MGLMMYDIILEPLITEKIARATESANQYGFKVHPKANKKEIKSA
                     VEKIFNVHVESVNTVNVDGKWRRVRYQPGQTPDWKKAIVTLKKGEKIDLTK"
     gene            1077..>1530
                     /gene="rplB"
                     /locus_tag="PLY30_03860"
     CDS             1077..>1530
                     /gene="rplB"
                     /locus_tag="PLY30_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPW65277.1"
                     /translation="MGLIKFRPVTPTRRYGNVSDFSEITVDRPYKPLTEIRKRTGGRN
                     NFGKITSRRIGGGHKQRIRLVDFRRSRFDVPAKVLTVEYDPNRSARIALIEYTDGQKS
                     YILCPDGLRVGEEVMSGPDVDVQVGNHLPLEKIPDGTMVHNIELQPGRG"
BASE COUNT          401 a          392 c          476 g          261 t
ORIGIN      
        1 cgcggcaaga agaatccatg gaaggtcagc aatcagaagg aatccccgaa agcttcctct
       61 gaaacgtccg cgaaacagga aactcaagag ggcgcgaagt aagcgtccga tccgaaaggc
      121 agaataagac gatgaaagtg cctctttata ccaaagaggg taagaagaag caggaggtgg
      181 tgttgaaccc cgacatcttc ggggtccgta tcaacgcccg cctgatcgag cttgtccgga
      241 acgcttacag cgccaacctt cgcaggggga cggccagtac caagacccgc ggggaagtcc
      301 gcggcggcgg caagaagccc tggaaacaga aggggaccgg gaatgcccgt cacggttcga
      361 accgctcccc gatctgggtc ggcggcggtg tcgtgttcgg ccccaggcca cgggattatt
      421 cagtccgtct ttcgaaggcg atccgaacga aggcgctcca ggtcgcgatc gcgatgaggg
      481 cgcgtgagaa gaacgtgctg ctgctcgaga ccctgaagct tgagacgccg aagacgaagg
      541 aatttgcggg gatcgtcaag acgcttccgc tcgggagcaa gcgagcggtc tatgtcacga
      601 aggagcagga tgtgaatatg aaacgggcaa gccgtaatct gcagaaatgg gtcgaggtca
      661 agaacgtggc ggatgtcagc gcctacgatg tgctgcagag cccgaaggtt atcattgacg
      721 aagcggcgct cgaaacgctc gagaaacgac tgacaggaga ttcgaaataa tgggcctcat
      781 gatgtacgac atcattctgg aaccgttgat cacggaaaag atcgcgaggg cgaccgaaag
      841 cgccaaccag tacggcttca aggtccatcc gaaggcgaac aagaaagaga tcaagtccgc
      901 ggtcgagaaa atcttcaatg tccacgttga gagcgtgaat acggtcaatg tggacggcaa
      961 atggcgccgg gtccgttatc agcccggtca gacgccggac tggaaaaaag ccatcgtaac
     1021 cctgaaaaag ggcgaaaaga tcgatttgac gaaataagga gctatcggaa actgtcatgg
     1081 gacttattaa attcaggcca gtcacgccga cgcggcgcta cgggaatgtg tcggattttt
     1141 ccgagattac ggtggaccgc ccctataaac cgctcacgga gatccgcaag cggaccgggg
     1201 gacgcaacaa cttcgggaaa atcacctcgc gtcgtatcgg aggtgggcac aaacaaagga
     1261 tccgcttggt ggatttccgg cgtagccgct ttgatgttcc cgcgaaggtc ctgactgtcg
     1321 agtacgatcc gaaccgtagc gcgcgcatcg cgctcattga atatacggac gggcaaaaga
     1381 gctacatcct gtgtccggac ggcctcaggg tcggtgagga ggtcatgagc ggtcccgatg
     1441 tcgatgtgca ggtcggcaat caccttccgc ttgaaaaaat cccggatggc acgatggtcc
     1501 ataacatcga gttgcaaccc ggccgtggcg
//
LOCUS       DAOOBX010000244         1522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_90481_length_1522_cov_2.690525, whole genome shotgun sequence.
ACCESSION   DAOOBX010000244 DAOOBX010000000
VERSION     DAOOBX010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90481_length_1522_cov_2.690525"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PLY30_03865"
     CDS             <1..799
                     /locus_tag="PLY30_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65278.1"
                     /translation="DIEATLDVLAEFSAPAACVIKHNNPCGIATDPDLVKAVEGAIDS
                     DALSAFGGIVGLNRSCDLKTAQKILEKLGFFEVLIAPSFKAAALKVLKERKNLRLIEV
                     DDFRDAYPYDFKFLRSGVLVQEKDRPIRERMAKLKKKLRFVTKAKLTGAEIDALLFAF
                     QCSKVVKSNAIILTQGKRTVGVGAGQMSRVDSMQIALSKAGYLAEGCYIGSDAFFPMP
                     DSIEIAAKHKVKAIIQPGGSIRDADVIAACDAAGIAMAFTGERHFRH"
     gene            874..>1522
                     /locus_tag="PLY30_03870"
     CDS             874..>1522
                     /locus_tag="PLY30_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HPW65279.1"
                     /translation="MNYSQSLSYLDSFLNLERLSHLPRNSFFNLRRMEHLLRIAGRPE
                     KKFFPILITGTTGKGSTGFFLESILKENGIAVGYYHSPHVEDVRERIRLDGRMAPEAL
                     WARGLSRVRTMLAKHPVPATLGCLTYFEIMTFLAILLFSETGRRIGIFEIGLGGRLDA
                     TNVLRAPLVILTPIHLDHTHFLGDTVAKIAREKAGIIKKHCCVISVRQTPEAARAI"
BASE COUNT          315 a          465 c          436 g          306 t
ORIGIN      
        1 cgatatcgag gcaacgctgg atgtcctcgc cgaattcagc gcgcccgcgg catgcgtcat
       61 caagcacaac aatccctgcg gcatcgccac cgatcccgac cttgtgaagg ccgtggaagg
      121 agcgatcgat tccgacgcgc tttccgcgtt cggcggaatc gtagggttga accgttcctg
      181 cgacctgaaa acggcgcaga agattctcga aaagctcgga ttcttcgagg tcctgatcgc
      241 cccgtcgttc aaagcggccg cgctcaaggt cctgaaggag cgcaagaacc tccgcctgat
      301 cgaggtcgac gatttccgcg acgcctatcc gtatgatttc aaattcctcc gcagcggtgt
      361 tcttgtgcag gaaaaagacc gtccgatccg ggagcgcatg gcgaagctga agaaaaaact
      421 ccggttcgtg acaaaggcga agctgaccgg cgccgagatc gacgcgctcc ttttcgcctt
      481 ccagtgttcg aaggtcgtaa agtcgaacgc gatcatcctg acgcagggca aacgcacggt
      541 cggcgtggga gcaggccaga tgtcgcgcgt cgattccatg caaatcgctc tctcgaaggc
      601 cggttatctc gccgaaggct gctacatcgg ttccgatgcc tttttcccga tgccggactc
      661 gatcgagatc gcggcgaagc acaaggtcaa ggccatcatc cagcccggcg gttccatcag
      721 ggacgcggac gtgatcgcgg cctgcgatgc ggccggcatt gccatggcgt tcacgggcga
      781 gcggcatttt agacattaaa tgaaacgcga cacatgccgc gaagccacga gggaattccc
      841 gtgacgcgcg gcatatggcc ttgtggctcg gaaatgaact actcccaatc cctcagctat
      901 ctcgattcat tcctgaatct ggaaaggctg agccatctcc cgcgaaattc tttttttaac
      961 ctgcgccgga tggaacatct gctccggatc gcgggccgtc ccgagaaaaa attctttccc
     1021 atcctcatca caggcacgac cggcaaaggt tccaccggtt tttttctcga atccatcctg
     1081 aaagagaacg gtattgccgt tggttattat cactcaccgc acgtggaaga tgtccgcgaa
     1141 cggatccgcc tcgacggacg gatggctccg gaagcgctct gggcccgcgg tctgtcccgg
     1201 gtccggacga tgctcgcgaa gcaccccgtg ccggccacgc tcggatgcct gacctatttc
     1261 gagatcatga cgttcctcgc cattcttctt ttttcggaga ctggccgccg tatcgggatc
     1321 ttcgagatcg gtctcggcgg aaggctcgac gcgacgaacg tcctccgggc gccacttgtg
     1381 atcctgacgc cgatccatct cgatcacacg cattttctcg gagacacggt cgcgaagatc
     1441 gcgcgcgaga aggcggggat catcaagaaa cactgctgtg tcatatcggt ccggcagacg
     1501 ccggaggccg cgcgggcgat cc
//
LOCUS       DAOOBX010000245         1512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_91268_length_1512_cov_2.934798, whole genome shotgun sequence.
ACCESSION   DAOOBX010000245 DAOOBX010000000
VERSION     DAOOBX010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91268_length_1512_cov_2.934798"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLY30_03875"
     CDS             <1..350
                     /locus_tag="PLY30_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65280.1"
                     /translation="RCFELRVAATGDDAVGHAAFLDAMPVREIFESYPGLPKPELKGK
                     LDGLMALARTKAEKLLSGGSANTRAAHTWLEAFDLVYDSKSAIEANANQKLMATRLAM
                     RLAKLFPSTKATL"
     gene            350..>1512
                     /gene="metG"
                     /locus_tag="PLY30_03880"
     CDS             350..>1512
                     /gene="metG"
                     /locus_tag="PLY30_03880"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPW65281.1"
                     /translation="MTKRYYLTTPIYYVNGKPHIGHAYTSILCDTFARYHRMLGDEVF
                     FLTGTDEHGIKVQKVATAEGMEPKAFVDGIVPKFRELWSLLGIRYEHFIRTTDDYHKT
                     GVQKVLGDLERKGDIYRAAYHGWYCTPCESFWTELQLKEGRCPDCGRDVERLEEENYF
                     FKLSSYQQFLIDHIRANPDFIQPDYRRNEILGFLRQPLEDLCITRPKARLTWGIEYPG
                     SPDHVVYVWFDALLNYLTGIGFGTDDARFRKFWPADCHMVGKDILRQHAVYWPIMLRA
                     MGVELPKRVYAHGWWKIGGAKMSKSLGNIVDPVEIVTKYGTDAFRYFLMREVTLGLDG
                     TYSEDLLLERYTSDLANDLGNLWHRTASMIEKYFEGKVRQVAPEVWQTKLMRDT"
BASE COUNT          335 a          397 c          464 g          316 t
ORIGIN      
        1 cgcgatgttt cgagctgcgt gtcgcggcga ccggggatga cgcggtgggg cacgccgcgt
       61 ttctggatgc gatgccggtg cgggaaatct ttgaatcgta tcccggactt ccgaaaccgg
      121 aactgaaagg caaactggac gggctcatgg cgctcgcccg cacgaaagcg gagaagcttc
      181 tgtcgggagg gtcggccaac acccgggcgg cccacacctg gctcgaggct tttgatcttg
      241 tctatgattc aaagtccgcg atcgaggcga acgccaatca aaaactgatg gcgacacgcc
      301 tcgcgatgag gcttgcaaaa ctttttccgt caacgaaagc gacgctctga tgacaaaacg
      361 gtattacctt acgacgccga tctattacgt gaatggaaaa ccccatatcg gccacgccta
      421 caccagcatc ctgtgcgaca cgttcgcgcg gtatcaccgg atgctcggtg acgaggtgtt
      481 ttttttgacg gggactgatg aacacggcat caaggtgcaa aaggttgcga cggccgaggg
      541 gatggaaccg aaggcctttg tggacggaat cgttccgaaa ttcagggaac tgtggtccct
      601 tctggggatt cgttacgagc atttcatccg gacgacggac gactatcaca agaccggcgt
      661 tcaaaaggtg ctgggcgatc tcgaaagaaa gggggacatc tacagggcgg cctatcacgg
      721 ctggtactgt actccctgcg aatcgttctg gacagaactc cagctgaagg aggggaggtg
      781 tccggattgc ggccgcgacg tggaacgtct ggaggaggag aactatttct tcaaactttc
      841 aagctatcaa cagtttctga tcgatcacat cagagcgaat cccgatttca ttcagccgga
      901 ctatcgcagg aacgagattc tgggattcct ccgtcagccg ctcgaagatc tttgcattac
      961 ccggccgaag gcccggctca cctgggggat cgaatatccg ggctcccccg atcatgtggt
     1021 ctatgtatgg ttcgacgcgc tgcttaacta tctgaccggt atcggattcg ggaccgatga
     1081 cgccaggttt cgcaagttct ggcccgccga ttgccatatg gtcggcaagg atatcctgcg
     1141 tcagcatgcc gtttactggc cgatcatgtt gagggcgatg ggcgttgaat tgccgaagcg
     1201 cgtgtacgcg cacggctggt ggaagatcgg cggggccaag atgtcgaaat cgctcggcaa
     1261 tatcgtcgat cccgtcgaga tcgtcacgaa gtacgggacg gacgcgttcc ggtatttcct
     1321 gatgcgtgaa gtaacgctcg gcctggacgg cacctattcg gaggaccttc tgctcgagcg
     1381 ttacacgagc gaccttgcga acgatctcgg aaatctgtgg caccggaccg cgtcgatgat
     1441 cgaaaagtat tttgaaggca aggtccggca ggtggcgccg gaggtctggc agacgaaact
     1501 catgagggac ac
//
LOCUS       DAOOBX010000246         1510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_91441_length_1510_cov_3.017182, whole genome shotgun sequence.
ACCESSION   DAOOBX010000246 DAOOBX010000000
VERSION     DAOOBX010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91441_length_1510_cov_3.017182"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1257)
                     /locus_tag="PLY30_03885"
     CDS             complement(190..1257)
                     /locus_tag="PLY30_03885"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPW65282.1"
                     /translation="MKATSIIIKLDHRDPDNARLKEAVRTCREGKIVGFPTETVYGIG
                     GVRSAPGIDQRLRDIKGRGEQKPFSLHIGDWDMIDRIGVVQTPAVRFFIRNFWPGPVT
                     LILNGTKGGTVGVRFPKNKVARRLICEAGEPFVATSGNKSGMPSPYTAQQVFEQFGNA
                     IDCLIDSGKTEFAHESTVLDLSGEEPVILRKGACASEVEKAVAMVKAGCYPKKKIIVV
                     CTGNSCRSPMGEGWLKDELERRGLADLIEVSSCGTNAREGLPAASEAEFVMKNRGVDL
                     SRHKSRLCRVHDIWCADLILVMSACHAGSIEELLPGSRAKIRNLDVEDPIGLGIDIYE
                     KTILELERKMKTNMNEIILLP"
     gene            complement(1275..>1510)
                     /locus_tag="PLY30_03890"
     CDS             complement(1275..>1510)
                     /locus_tag="PLY30_03890"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPW65283.1"
                     /translation="DLNGMDALLATVQMPAGIPVATVAVGKPGARNAALLAAQILGLS
                     DAGVAGKLGAYKKKMAEACREKNQKLQNKLKET"
BASE COUNT          266 a          497 c          399 g          348 t
ORIGIN      
        1 ttcgagacca tctccgcggc cttgaacatg aaatcggggt aatcgcagga atcctccgaa
       61 ttcgtcccgc agtcgacgac attaaagttc ttcttctgga ggtggctaat cagcgcgttc
      121 ttcatttgaa gtccgcggtg gtcgcacgcg atggcaatct tgggtctcat catgataagg
      181 tcggcccttt cagggaagca ggatgatctc gttcatgttc gttttcattt ttctttcgag
      241 ctcgaggatc gttttttcat aaatgtcgat cccgagaccg atcgggtcct cgacgtccag
      301 gttccggatc ttcgcgcggg accccgggag cagttcctcg attgaacccg catggcacgc
      361 cgacatcaca aggatcaggt ccgcgcacca gatatcgtgg acccggcaca accggctctt
      421 gtgacgactg aggtcgacgc cgcgattctt catcacgaat tcggcttcgc tcgcggccgg
      481 caggccctcg cgggcgttgg tgccgcagga ggaaacctca atcagatcgg ccagcccccg
      541 gcgctccagt tcgtccttca accacccttc tcccatcggg gagcggcagg aattcccggt
      601 gcagacaacg atgatctttt ttttcggata acaccccgcc ttcaccatcg ccacggcctt
      661 ctcgacctcg gaggcacacg cgcccttcct gagaatcacg ggttcctccc cgctcaggtc
      721 gagcacggtc gattcatggg cgaactccgt ttttcccgag tcgatcagac aatcgatcgc
      781 gttcccgaac tgttcaaaga cctgctgggc cgtgtacggc gacggcatcc cgcttttgtt
      841 accgctcgtc gcgacgaacg gctcgccggc ctcgcagatc agacgccttg ccaccttgtt
      901 cttcgggaac cggacaccga ccgtcccgcc tttcgttccg ttcagaatca gcgtgacagg
      961 tcccggccag aaattcctta taaaaaagcg caccgccggc gtctgaacga ctccgatcct
     1021 gtcgatcatg tcccagtcgc cgatatggag tgagaaaggc ttttgttccc cgcgcccctt
     1081 gatgtcgcgc agccgctgat cgatacccgg cgccgaccgg acgccgccga taccgtagac
     1141 cgtctcggtc gggaacccca cgatcttgcc ctcacgacag gtcctgacag cttccttcag
     1201 cctggcgtta tccggatccc ggtgatccag ctttatgatt atggaggtcg ctttcatcgc
     1261 tcgtgttttc cgtgtcaggt ctcttttaat ttattctgga gtttctggtt cttttcccgg
     1321 caggcctcag ccatcttttt tttataggca cccagcttcc ccgcgacccc ggcgtcagaa
     1381 agtccgagga tctgcgccgc cagcagcgcc gcgttccggg cgcccggctt cccgaccgcc
     1441 accgtcgcca ccgggattcc cgcgggcatc tgaacggtcg cgagcaaggc gtccatcccg
     1501 ttcaggtccg
//
LOCUS       DAOOBX010000247         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_91962_length_1504_cov_2.269151, whole genome shotgun sequence.
ACCESSION   DAOOBX010000247 DAOOBX010000000
VERSION     DAOOBX010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91962_length_1504_cov_2.269151"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PLY30_03895"
     CDS             <1..1153
                     /locus_tag="PLY30_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPW65284.1"
                     /translation="FFDDSLRGWRGGEQLEVDARGKVVQVLSRRPPEPGADLRLTLDL
                     DFQKKIMDLIKGQHASVAVMDLETEGLLAMASSPAYDPNIFVSPSASGRRLSVLKDIN
                     SPMLDRSIGAAYPPGSVFKLVTALAALELGKITPQTRFYCNGKFKLGKSGRVAKCWFE
                     GGHRSINLYEAIERSCNVYFYNLSTKLSPDDIAKYAHELGLGEPMRLEISRLTPGLIP
                     DAAWKRSRFKQPWYQGETLNMAIGQGYVLVTPLEILRLTAIIAKNGKFVDPHLTQNDA
                     AVGEPLRVAIREENLNVIKRAMLQVVESDYGTGQLARVDFDKLAAKTGTAQAPQKAHS
                     WMTGFFPYKNPKIAFVVFVEHGGSGGITSARIVKQILEVWKGVDAPKAV"
     gene            1134..>1504
                     /locus_tag="PLY30_03900"
     CDS             1134..>1504
                     /locus_tag="PLY30_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPW65285.1"
                     /translation="MLQKLFKDLHLPLILAVIGLAALGLFFIYSASFRDPGQYEVKQI
                     FWIAIGLAVMIAVPYLGYRPFISVAYLFYGFSILLLILVYFVGSTRLGAQRWLTLGPF
                     VIQPSEFAKLGTLLALVNYLG"
BASE COUNT          291 a          434 c          453 g          326 t
ORIGIN      
        1 attctttgat gacagcctcc gcggctggag ggggggagag caactcgagg tggatgcccg
       61 cggaaaagtg gtgcaggtgc tttcccggcg gccgcccgaa ccgggtgccg atctgaggct
      121 gaccctcgat ctggatttcc agaaaaagat catggatctg atcaagggac agcatgcgag
      181 cgttgccgtc atggacctcg agaccgaagg gcttctcgcg atggcgagtt cgcccgcgta
      241 tgatccgaat attttcgtaa gcccttccgc gagcggccgc cgtctgtccg tcctcaagga
      301 tatcaatagc cccatgctgg accgttcgat cggggcggcc tatcccccgg gatcggtctt
      361 taagctcgtg acggcgcttg ccgcgctcga gctcgggaag atcacgccgc agacccgttt
      421 ctactgcaac gggaagttca aactcggcaa atccggacgc gtggccaagt gctggttcga
      481 gggagggcac cgcagcatca atctctatga ggccatcgaa cgttcgtgca atgtttactt
      541 ctacaacctc tccacgaaac tctctccgga tgatatcgcg aaatacgcgc acgagctcgg
      601 tcttggtgag ccgatgagac ttgaaatcag tcgcctgacg ccgggactga ttccggacgc
      661 cgcctggaag cgctcgcggt tcaaacagcc atggtatcag ggggagacgc tcaacatggc
      721 gatcggtcag ggctatgtcc tggtcacgcc gctcgagatc ctgcgactga ccgcgatcat
      781 cgccaagaac gggaaattcg tcgatccgca tctgacgcag aacgacgcgg ccgtcgggga
      841 accgttgcgc gtcgcgatcc gggaagagaa cctgaatgtg atcaaacggg cgatgctgca
      901 ggtcgtggaa tccgactacg ggacggggca gctcgcgcgc gttgattttg acaaactggc
      961 tgccaagacc gggacggcgc aggctcccca gaaggcccat agctggatga cggggttctt
     1021 tccctacaaa aatccgaaga tcgccttcgt cgttttcgtc gagcacggcg gttccggcgg
     1081 tatcacgagc gcgaggatcg tcaaacagat tctcgaggtc tggaagggtg tcgatgctcc
     1141 aaaagctgtt taaagacctt catctgcctt tgatcctcgc ggtcataggt ttggccgcgc
     1201 tcgggctctt tttcatctac agcgcctcgt tccgtgatcc cgggcaatat gaggtcaaac
     1261 agatcttctg gatcgccatc gggctggccg tcatgatcgc ggtgccgtat ctcggttacc
     1321 gcccgttcat cagtgtcgcg taccttttct acggttttag catccttctg ctgatcctgg
     1381 tctatttcgt cggctcgacg cgtctgggcg cgcagcgatg gctgacgctc ggaccttttg
     1441 tgattcagcc gtcggaattc gcgaagctcg gcacgctgct cgcgcttgtc aattacctcg
     1501 ggag
//
LOCUS       DAOOBX010000248         1500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_92287_length_1500_cov_3.190311, whole genome shotgun sequence.
ACCESSION   DAOOBX010000248 DAOOBX010000000
VERSION     DAOOBX010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92287_length_1500_cov_3.190311"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLY30_03905"
     CDS             <1..539
                     /locus_tag="PLY30_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HPW65286.1"
                     /translation="DGTVLGYGTETGAFSRLDDLKPGFALGQIDRAIIMNPYQVNARI
                     ALPITTFANVVKGHTVDFVLYANNYEDIDEDHPVIERFETSEEALKTFRDGAVMSKGT
                     TRTMGLVHTYFSNVFGPVQYRAEHDVLARKFFEAFYKKGIFVGQMRTRLGVPGWERKG
                     PEESAKALLDLIRNDQRY"
     gene            621..>1500
                     /locus_tag="PLY30_03910"
     CDS             621..>1500
                     /locus_tag="PLY30_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015228991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HPW65287.1"
                     /translation="MTKEVSICLHGHFYQPPRENPWIEEIEQQDSAAPFHDWNERIHY
                     ECYLPNSRARALDAKGKIVDIVNNFEHVSFNFGPTLLSWLDAKHPDTYKSIIRADQVS
                     RELHHGHGNAIAQVYNHMILPLANLRDKRTQIRWGLEDFRYRFGRESESIWLPETAVN
                     EDTLEALVAEKIKYIILSPYQARAVRPLDGGEWKEVTGGHIDPRRPYRCFLKNNPALF
                     IDIFFYDGPISRAVGFDSLLFDAKLFTDRLQSAVVDNDEPQLIHLATDGETFGHHKPF
                     GDRVLGYLTYVEIPKRG"
BASE COUNT          326 a          456 c          433 g          285 t
ORIGIN      
        1 aagacgggac ggtcctcggc tacgggacgg agacgggcgc tttttcgagg cttgacgacc
       61 tgaaacccgg gttcgcgctc ggccagatcg atcgcgcgat catcatgaac ccctatcagg
      121 tgaacgcccg catcgcgctc ccgatcacga ccttcgcgaa cgtcgtcaag ggccacaccg
      181 tggattttgt cctgtacgcc aacaactacg aggacatcga cgaggaccac cccgtaatcg
      241 agcgtttcga gacctccgaa gaggccctga agacctttcg cgacggggct gtcatgagca
      301 agggcacgac ccggaccatg ggacttgtgc acacttattt ctcgaacgtt ttcggacccg
      361 tgcagtaccg ggccgagcac gatgtcctgg ccaggaaatt ctttgaagcc ttctacaaaa
      421 aaggtatatt cgtgggacag atgcgcacgc ggctcggcgt cccggggtgg gaacgcaagg
      481 gtcccgagga atccgcgaag gcgcttctgg acctgatccg taacgaccag aggtattgaa
      541 taatcaggat caggaatgag agatgagctt tccggcccgg agctcatgga acagtccccg
      601 ggcccgagtt ttggaggacc atgaccaagg aagtttcgat ttgtctgcac gggcatttct
      661 accagccccc ccgcgagaat ccctggatcg aggagatcga gcagcaggac agcgccgctc
      721 ctttccatga ctggaacgag cgtatccact acgagtgcta cctcccgaac tcccgcgcgc
      781 gcgccctcga cgcgaaggga aagatcgtcg atatcgttaa taatttcgag cacgtcagtt
      841 tcaacttcgg gccgacgctt ctctcctggc tcgacgccaa gcaccccgat acctacaaaa
      901 gcatcatccg cgcggaccag gtgagccggg aactgcacca cggtcacggg aacgcgatcg
      961 cgcaggtcta caaccacatg atcctcccgc ttgccaacct gcgggacaag cggacgcaga
     1021 tccggtgggg actcgaggat ttccgttatc gctttggccg cgaatcggaa agtatctggc
     1081 ttcccgaaac ggccgtgaac gaggatacgc tagaggcgct tgtcgcggaa aagataaaat
     1141 acatcattct ctcgccgtac caggcccggg cggtgcgacc gctcgacggc ggtgagtgga
     1201 aagaagtgac gggcgggcac atcgatccgc gccggcccta ccggtgtttc ctgaagaaca
     1261 atccggctct ttttatcgac atcttttttt acgacggtcc gatctcgcgc gccgtcggtt
     1321 tcgacagcct ccttttcgat gcgaaacttt tcacggaccg tcttcagagc gctgtcgtgg
     1381 acaacgacga gccccagctt atccatctcg cgacggacgg cgagaccttc gggcatcaca
     1441 agccgttcgg agaccgcgtg ctcggctacc tgacgtatgt ggagatcccg aagcgcggcc
//
LOCUS       DAOOBX010000249         1480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_94087_length_1480_cov_2.875789, whole genome shotgun sequence.
ACCESSION   DAOOBX010000249 DAOOBX010000000
VERSION     DAOOBX010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94087_length_1480_cov_2.875789"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..406
                     /gene="rplV"
                     /locus_tag="PLY30_03915"
                     /pseudo
     CDS             32..406
                     /gene="rplV"
                     /locus_tag="PLY30_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            577..1344
                     /gene="rpsC"
                     /locus_tag="PLY30_03920"
     CDS             577..1344
                     /gene="rpsC"
                     /locus_tag="PLY30_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPW65288.1"
                     /translation="MGQKAHPYGLRVGFIKPWRARWFAKRDAVAFLGEDLRIREYLKA
                     KLATAGVSSVEIDRAASRLRVRIFAAKPGVIIGRRGQEIDKIKEDLAAITKNEVFLDI
                     KEVKMPAIDAQLVAENIALQLEKRIGFRRAMKKAVQLAMQKGALGIKVKCKGRLGGAE
                     IAREEGYKEGKVPLSTFRADIDYGFAEAFTTYGTIGCKAWIYKGDVLVKKEQEEKKQE
                     LEKKLSQVEAEMMKSAAPVEEKPAVETEEKTNDAPDA"
     gene            1328..>1480
                     /locus_tag="PLY30_03925"
     CDS             1328..>1480
                     /locus_tag="PLY30_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L16"
                     /protein_id="HPW65289.1"
                     /translation="MPLMPKRVKYRKQHRGWMGGVATRGSELAFGEYGLKALECAWLT
                     AIQIEAS"
BASE COUNT          365 a          368 c          482 g          265 t
ORIGIN      
        1 gtgccaaaac ctaaggatag gcggacttat catggctaag accgaaagca aaaagagtga
       61 agcgcccgtg gccccggacc ctcgcaaggt gaaggcggtc acgaaatacg tccggatcgc
      121 gccgcgcaag ctgcgtcttg ttatcgatac gatccgcaaa cgtcccgctc tcgaggcggt
      181 cggaattctt tccctgatgc cccggaaggg cgccaggatc gcggttaaga cgctcaggag
      241 cgccattgcc aacgcgaaaa atctcggtct tgatgaaaaa cgattgatcg tttctgacat
      301 ccgcgcggac ggtggtcccg tgatgaagcg gttcatgagc cgatcgaagg gccgtgccga
      361 gcggatcctg aaacgcagtt cgcatctcac ggtcatcgta tccgagggcc gtaaggtttg
      421 gggcggcgaa gcgcctgaag aggcgaaagc caaaaagaaa caggccgtga aagccgcttc
      481 ctgataagcg atttgaaatt acggaatttc agcactgaac tgatcttccg gttcgaaatg
      541 ctaaaaagaa gatcagtgta aagaggagga agtaccttgg gtcagaaagc gcatccgtac
      601 ggattgaggg tcgggttcat caagccctgg agagcccgct ggtttgcgaa acgcgacgcg
      661 gtcgcttttc tcggcgagga tctcaggatc cgggaatatc tgaaggccaa gctggccact
      721 gccggtgtct cgtcggtcga gatcgaccgt gccgcgagcc gcttgcgcgt ccgtatcttt
      781 gccgcgaagc ccggtgtgat tatcggtcgc cgcggacagg agatcgacaa gatcaaggaa
      841 gacctggcgg cgatcacgaa gaatgaggtg ttcctcgaca tcaaggaagt caagatgccg
      901 gcgatcgatg cgcagctcgt tgccgaaaac atagcgctcc agcttgaaaa acgcatcggg
      961 tttcgccgcg cgatgaaaaa ggcggttcag ctcgcgatgc agaagggcgc gctcgggatc
     1021 aaggtcaagt gcaaggggcg gcttggtggc gccgagattg cccgcgaaga agggtacaag
     1081 gagggcaaag tgccccttag caccttccgg gccgatatcg attacggatt tgccgaagcg
     1141 ttcacgacgt acgggacgat cggctgcaag gcgtggattt ataagggtga cgttcttgtg
     1201 aagaaagaac aggaagagaa gaaacaggag cttgaaaaga agctcagcca ggtcgaagcc
     1261 gagatgatga agtcggcggc gccggttgag gagaaaccgg ctgtggagac cgaggagaag
     1321 acgaacgatg cccctgatgc ctaagcgtgt aaaataccgc aagcagcatc gcggctggat
     1381 gggtggtgtt gccacccgcg gcagcgagct cgcgttcggt gagtatgggc tcaaagcgtt
     1441 ggaatgcgca tggctcacgg cgatccagat cgaggcgtcg
//
LOCUS       DAOOBX010000250         1478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_94282_length_1478_cov_2.508082, whole genome shotgun sequence.
ACCESSION   DAOOBX010000250 DAOOBX010000000
VERSION     DAOOBX010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94282_length_1478_cov_2.508082"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..473
                     /locus_tag="PLY30_03930"
     CDS             126..473
                     /locus_tag="PLY30_03930"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW65290.1"
                     /translation="MHKKNHARESETREAMRGGAGKITVRHYFKPGDLKARTRLCAEL
                     VIPAGASIGAHDHVDEDEIYIIHRGKGIMTDGGEEFEVAAGDAVVTGQGAFHSIRNTG
                     TEDLLITAVIIKY"
     gene            646..>1478
                     /locus_tag="PLY30_03935"
     CDS             646..>1478
                     /locus_tag="PLY30_03935"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPW65291.1"
                     /translation="MEFPGKAASFGSRGVIVHGASVEKNGLKSSLVPLLKKAGVETAW
                     FCRGKAHPHALQEASVQEIRDVIALGKNTNARWIAGIGGGSVLDLAKAAAGLYHAHKD
                     PVYYQEGGKLTEKGIPFLAVPTTAGSGSEATLNSVIINPVKKMKLSIRHPAFLASLVI
                     LDPVLLKSLPREVIVHSGMDAFVQAYESYISKNASLFTETLALRAIEMIASRLPGAAR
                     TKNEDALHEMLFASFLTGIAFSHSRLGVIHGIAHPLGVLYDIPHGLVCSACLIPSIKL
                     NR"
BASE COUNT          324 a          473 c          388 g          293 t
ORIGIN      
        1 ctccgtcagg gagaagaacg gcagcctcaa ggaattcacg gtcgaacacg gtttcttcga
       61 atttaacggg aaccgggctc tattcctcac ccattcctgt tcaccggtca aataaagaat
      121 ctgccatgca caaaaagaat catgcccgcg aatccgagac acgcgaggcg atgcgcggag
      181 gggccggcaa gatcaccgtc cgccactact tcaagcccgg ggatctcaag gcccgcacgc
      241 gtctttgcgc ggagcttgtc atccccgcgg gcgcgagtat cggcgcccac gaccatgtgg
      301 acgaggacga aatttacatc atccaccggg gtaaaggcat tatgacggac ggtggcgagg
      361 aattcgaggt tgcggcgggc gatgccgtcg taaccggaca gggcgcgttt cattccatcc
      421 ggaacaccgg cacggaagac ctcctcatca cggccgtcat cataaagtat taacttcatg
      481 acatcacgcg cgccagaccg catgcctcac ggaacatctt tgatgggaca gccgtatcct
      541 gcaaccttgc ggcgcgcggc tttgagcctg atgtaactca tgaccggcgc cttcccttcc
      601 gagatccaca tcccggacaa ggtcctttcg gggaaaaatg gcgtcttgga atttccgggc
      661 aaagcggcct cgttcggctc ccgcggcgtg atcgttcacg gcgcttccgt cgaaaagaac
      721 ggactcaaat ccagtctcgt tcccctttta aagaaagccg gtgtcgaaac cgcctggttc
      781 tgccgcggga aggcgcaccc ccacgcgctc caggaagctt ccgttcagga gatccgcgac
      841 gtgatcgcgc tcggcaaaaa cacgaacgcc cggtggatcg ccgggatcgg cggcgggagt
      901 gtccttgatc tcgcgaaagc cgcggcgggc ctttatcacg ctcacaagga ccccgtctat
      961 taccaggaag gcgggaaact cacggaaaaa ggaatcccgt tcctcgccgt ccccacaacc
     1021 gccggcagcg gctccgaagc cacgttgaat tcggttatta tcaaccccgt aaaaaagatg
     1081 aaactctcga tccgccatcc ggcatttctt gcctcgctcg tcatcctgga cccggtactt
     1141 ttaaaaagcc tgccgcgcga agtgatcgtc cattccggca tggacgcttt tgtccaggct
     1201 tatgagtcct acatttcaaa aaacgcttcc ctcttcacgg aaaccctcgc cctccgggcc
     1261 attgaaatga tcgcctcccg gctccccggc gccgcaagga caaagaacga agacgcactg
     1321 cacgaaatgc ttttcgcgag cttcctcacg gggatcgctt tttcccattc ccgcctcgga
     1381 gtgattcacg gaatcgcgca tcccttgggc gttctttatg atatccccca cgggctcgtc
     1441 tgctcagcct gcctgatccc ttcgatcaaa ctgaaccg
//
LOCUS       DAOOBX010000251         1473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_94679_length_1473_cov_3.392102, whole genome shotgun sequence.
ACCESSION   DAOOBX010000251 DAOOBX010000000
VERSION     DAOOBX010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94679_length_1473_cov_3.392102"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /gene="purB"
                     /locus_tag="PLY30_03940"
     CDS             complement(<1..997)
                     /gene="purB"
                     /locus_tag="PLY30_03940"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPW65292.1"
                     /translation="MIPRYTRPEMAKVWTEENKLQKWLEVELAALDGLARYGYIPKEI
                     PAKVRPRAKFDVARVKEIEKITNHDVIAFLTSVAEHVGPAGRYVHFGLTSSDILDTGL
                     ALQLVDASRVLNRELRKLIDILAVRARAHANTLMVGRSHGVHAEPVTFGLKMALFHAE
                     FKRNLERFERATENIRFGKISGAVGTFANVAPKVENYVCKKLKLNPAPVSTQILQRDR
                     HAEYMTVIAVIGGSLEKLATEIRGLQKTECYEVQEYFAKGQKGSSAMPHKRNPITCER
                     IAGLARILRGNALAALENIALWHERDITHSSVERVIFPDSTILLDYMLGLMQNVIE"
     gene            complement(1016..1093)
                     /locus_tag="PLY30_03945"
     tRNA            complement(1016..1093)
                     /locus_tag="PLY30_03945"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1055..1057),aa:Glu,seq:ctc)
     gene            complement(1264..>1473)
                     /locus_tag="PLY30_03950"
     CDS             complement(1264..>1473)
                     /locus_tag="PLY30_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65293.1"
                     /translation="VLALLPAAVAADTVQAASDDYTTIQKDLTQDRLSDALRRIQMLE
                     QENRFLAKRVELLERNVNDIKNDRY"
BASE COUNT          298 a          431 c          442 g          302 t
ORIGIN      
        1 tctcgatcac gttttgcatg agccccagca tgtagtcgag gaggatcgtg ctgtccggga
       61 aaatgacgcg ctcgacggac gagtgcgtga tgtcacgctc atgccagagc gcgatattct
      121 cgagcgccgc gagcgcgttc ccgcggagga tacgcgcgag gcccgcgatg cgttcgcagg
      181 tgatggggtt gcgcttgtgg ggcattgccg aagacccctt ctgtcctttc gcgaaatact
      241 cctgcacctc gtagcattcg gtcttctgga gcccgcggat ctcggtcgcg agcttttcga
      301 gcgacccgcc gatcacggcg atcacggtca tgtactcggc gtggcggtcg cgctgaagaa
      361 tctgcgtcga cacgggcgcc gggttgagct tgagcttttt gcagacataa ttctcgacct
      421 tcggggccac gttcgcaaac gtaccgaccg cgcccgagat cttcccgaag cggatgttct
      481 ccgtcgcgcg ctcgaagcgc tcgaggttcc gtttgaactc ggcatgaaaa agggccatct
      541 tgaggccaaa ggtcacgggc tcggcgtgca cgccgtgcga gcgaccgacc atgagggtgt
      601 tcgcgtgcgc cctggcccgg acggcgagga tatcgatcag cttcctgagt tcccggttca
      661 gaaccctgga ggcgtccacg agctggagcg caagtcccgt gtcgaggata tcggaggagg
      721 tcagaccgaa atggacatag cgcccggcgg ggccgacatg ctccgcgacg ctcgtgagga
      781 acgcgatcac gtcatggttc gtgatcttct cgatctcctt cacgcgcgcg acgtcaaatt
      841 tcgccctggg gcgtaccttg gcgggaatct ctttggggat gtaaccgtag cgggcaaggc
      901 catcgagtgc cgcaagctcc acctccagcc atttttgaag tttattctct tcggtccaga
      961 ccttggccat ttcgggccgg gtgtagcgcg gaatcacaaa aaacctcact ataattggtg
     1021 gcgctaacgg gatttaaacc cgtgtttttg ccttgagagg gcaacgtcct agatcgggct
     1081 agacgatagc gccgcgaaaa ccataccgga ttattcaaca gacaccgcaa aatcgcaccg
     1141 agcaaaatac acgatgcccc agacttgcct gattgttcag cgaaaaacag gtcatgggag
     1201 gaaaaacacc aaaggcggcg gcattctacc gatggcatcc acccttgtca atcgaacgaa
     1261 ccgttaatac cggtcgttct tgatatcgtt gacattccgc tcgagcagtt cgacgcgctt
     1321 cgcgaggaaa cgattctcct gctccagcat ctggatgcgc cgcagggcat ccgaaagacg
     1381 gtcctgtgtc aggtccttct ggatcgtcgt gtaatcatcg gacgccgcct ggaccgtgtc
     1441 agccgcgacg gccgcgggga gcaacgccag aac
//
LOCUS       DAOOBX010000252         1471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_94921_length_1471_cov_2.216808, whole genome shotgun sequence.
ACCESSION   DAOOBX010000252 DAOOBX010000000
VERSION     DAOOBX010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94921_length_1471_cov_2.216808"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLY30_03955"
     CDS             <1..490
                     /locus_tag="PLY30_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPW65294.1"
                     /translation="AVRRAKFRGSHDNNDAVKDADIVIAAASAPQSIVEISSFKPGAI
                     VCDVGYPKNIAYAETDRHDILIFAGGICELPVEFNTGFDIGLPSRRVLYGCFSEAMVL
                     ALEGRYENYSWGKGHITKEKMDEILRLANKHGFRLAPFFWGHRQLIDQEIIAIGKMAE
                     KS"
     gene            609..>1471
                     /locus_tag="PLY30_03960"
     CDS             609..>1471
                     /locus_tag="PLY30_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPW65295.1"
                     /translation="MKVLVTGGTGFIGSTLVRHLLAAGYQVRVLVRQRRDSLLLEHLD
                     VEVVNGNVTSLQAVETAVQGCSIVFNLASLYSFYPFWIKNPKAMYRINVDGTVHMLSA
                     SLKYGVRKFVHTSTIATINARSDGQPADESAGFRERGASHYARSKFLAEQEVLKYCAK
                     GLPAVILNPAIVIGERDHKPTPTGDVIVKFLNQSYPGYFDTRWSVADADDVAQAHIAA
                     AKQGRIGERYILANPDHPTLKEIFRALEQVSGVRAPRIRIPYWALFCFTYIDEFLSHY
                     AFRKKPLMPTEG"
BASE COUNT          297 a          397 c          447 g          330 t
ORIGIN      
        1 ggccgttcgc cgtgcgaagt ttcgcggcag ccacgacaac aacgatgcgg tgaaggacgc
       61 ggatatcgtg atcgccgccg caagcgcgcc gcaatccatc gtggagatct cgagcttcaa
      121 accgggcgcg atcgtgtgcg acgtcgggta tcccaagaac atcgcttatg cggaaacgga
      181 ccgtcacgac atcctgatct tcgcgggcgg catttgcgaa cttcccgtgg agttcaatac
      241 gggattcgat atcggactgc cgtctcgcag ggtgctctac ggttgttttt ccgaagccat
      301 ggtcctggcg ctcgaaggcc ggtatgagaa ttattcgtgg gggaagggac atatcacgaa
      361 agagaagatg gatgagattc tccgccttgc gaacaagcac gggttccgcc tggcgccctt
      421 tttctggggg catcggcagc tgatcgatca ggagatcatc gcgatcggta aaatggcgga
      481 gaagtcgtag tggcgggatg cgtttcccaa ccggggtttg gtcccgtgga aacgatagcg
      541 gtcacgcgaa tccggatcgg gggcatcccg gagttgcccc ccggtctgtt ccgggggtgt
      601 ttgagatcat gaaagttctg gtgacgggcg gcacgggttt tatcggttcg acccttgtcc
      661 gtcatctgct cgccgccgga tatcaagtcc gggtcctggt gcgccagcgt cgggacagtc
      721 tcctgctcga acatcttgat gttgaagtgg tgaatggcaa tgttacgagc cttcaggccg
      781 ttgaaacggc cgttcaggga tgttcgatcg tttttaatct cgcctccctt tactcctttt
      841 atcctttctg gatcaaaaac cccaaagcca tgtaccggat caacgtggat ggcacggtcc
      901 atatgctgtc ggcctctctg aagtacgggg tccggaagtt tgttcatacc agtacgatcg
      961 cgacgatcaa cgcgcgaagc gacgggcaac ccgcggatga aagcgccggt ttcagggagc
     1021 gcggcgcaag ccattatgcc cgctccaagt ttttggccga acaggaagtt ttgaagtatt
     1081 gcgcgaaagg gcttcccgcg gtgatcctga atcccgcgat cgtgatcggt gagcgcgatc
     1141 ataagcccac cccgacgggc gatgtgatcg tcaaattcct gaatcaaagc tatccggggt
     1201 attttgacac ccggtggagc gtggcggatg ccgatgacgt tgcccaggcc catatcgccg
     1261 ccgcaaagca ggggaggatc ggcgagcgtt acattctcgc gaacccggat catccgaccc
     1321 ttaaagagat ctttcgcgcg ctggagcaag tttccggggt ccgggcaccg agaatccgga
     1381 ttccttactg ggctcttttt tgcttcacgt atatcgatga gttcctttcg cattatgcgt
     1441 tccggaaaaa accgctcatg ccgaccgagg g
//
LOCUS       DAOOBX010000253         1455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_96324_length_1455_cov_3.642857, whole genome shotgun sequence.
ACCESSION   DAOOBX010000253 DAOOBX010000000
VERSION     DAOOBX010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96324_length_1455_cov_3.642857"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLY30_03965"
     CDS             complement(<1..863)
                     /locus_tag="PLY30_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65296.1"
                     /translation="MRKAVIAACFMITLTVSARVWSAPQLRLAAPPRNASTAQTFLYE
                     VRVEWPVAEGAYEVSPTAPVFENLDLIAQSQSQETTGTVSRLILQFTLRGIQKGPARI
                     GTLDIRYRLAPSTEWRVLPVPDQTVQIAAAFPWKGVIGSVALAILLPLGSFFGIKGFK
                     AYRERERIKNLPPPDPKQRVYAKAEETILTYKGETQRDTIDHWTEAVKGTAITYYDLA
                     LKSATGRDILEALKGKSLTAKESSEITALFRRIEELRYAGNALPVREMELLQKTFLQY
                     VRGKIIIENPPR"
     gene            complement(897..>1455)
                     /locus_tag="PLY30_03970"
     CDS             complement(897..>1455)
                     /locus_tag="PLY30_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPW65297.1"
                     /translation="GIPPAPRGVPQIEVTFDIDANGIVHVSAKDLGTGKEQKIRIESS
                     SGLSKDEVEKLVKEGQAHAEEDKKKHEVITARNQLDSLIYQTEKALKDHGDKVSEDEK
                     KKVEEAIKEAKTAVTADSKETLEQAAQKLATASHTIAQKMYEEAAKKTAAKGGEAKSD
                     KKDDGVVDADYEVVDDDDKDKRKEQ"
BASE COUNT          234 a          423 c          430 g          368 t
ORIGIN      
        1 cggggtggat tttcaatgat aatcttgccc cgaacatatt gcaagaacgt cttttgcagt
       61 aattccatct cgcggaccgg aagcgcgttg ccggcgtacc tcagttcctc gatccgtcgg
      121 aaaagagccg tgatttcact cgattccttg gccgtgaggg acttcccttt gagcgcttca
      181 aggatgtcgc gtccggtcgc ggattttaag gccaggtcat aataagtgat ggccgttcct
      241 ttgacggcct ccgtccaatg atcgatcgtg tcccgctggg tttccccttt gtaggtcagg
      301 atcgtctctt cagccttggc atacacgcgc tgtttggggt cggggggcgg aagatttttg
      361 atcctttcgc gctcgcgata cgccttgaat cctttgatcc cgaagaacga gccaagcggc
      421 aacaagatcg cgagagcgac gctcccgatc acgcctttcc acgggaaggc ggcggcgatc
      481 tgaacggtct ggtccgggac cggaagcaca cgccattcgg tcgagggggc caggcggtag
      541 cggatatcga gagtgccgat cctggccggg cccttttgaa tgccgcggag cgtgaattga
      601 aggatcaaac gtgaaacggt gccggtggtt tcctgggact gggattgcgc gatgaggtcc
      661 agattctcga agacaggggc ggtggggctc acttcataag cgccttccgc cacgggccat
      721 tccacgcgga cttcgtaaag gaaggtttga gcggtcgagg cgttcctggg cggggccgcc
      781 agacgcagct ggggagcgga ccagacgcga gcggagaccg tcagtgttat catgaagcat
      841 gccgcgatca ccgcttttcg catcgaatcc gctcccctgc caaagatcag tggacgttac
      901 tgctcttttc tcttgtcctt gtcgtcatca tcgaccacct catagtccgc gtcgacaacg
      961 ccgtcgtcct tcttgtcgct tttggcctcg ccgcctttcg ccgcggtctt cttggcggcc
     1021 tcctcgtaca tcttctgggc gatcgtatgt gaggcggtcg cgagtttttg cgcagcctgt
     1081 tcgagcgtct ctttcgagtc cgccgtgacc gcggtcttgg cctccttgat ggcttcctcg
     1141 accttctttt tctcatcttc ggagaccttg tcgccgtggt ccttcagggc tttttcagtc
     1201 tggtagatga gactgtcgag ctggttacgc gcggtgatga cctcgtgttt cttcttgtcc
     1261 tcttccgcat gcgcctgccc ttccttgacc agtttctcaa cctcgtcttt cgaaagcccg
     1321 gaagaggact cgatccggat tttctgctcc ttgcccgtcc cgaggtcctt cgcggagacg
     1381 tgcacgatgc cgttcgcgtc gatgtcgaag gtcacttcga tctgcggtac tccgcgcggc
     1441 gcgggaggga ttcct
//
LOCUS       DAOOBX010000254         1453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_96534_length_1453_cov_2.510730, whole genome shotgun sequence.
ACCESSION   DAOOBX010000254 DAOOBX010000000
VERSION     DAOOBX010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96534_length_1453_cov_2.510730"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PLY30_03975"
     CDS             <1..726
                     /locus_tag="PLY30_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPW65298.1"
                     /translation="PALLGDIMPRGGLAVLIIPIDFEAPKGRIILPQVQAIRDALDHG
                     SMTLVVRESEYKAALARLKSVPDLVVCDSQVVDRMVAETPAEVKCTTFSILFSRLKGD
                     IVEMTRGALAIKNLRPGDKVLISESCSHHAIDGDIGRIKIPAWLRKYLGHDVTVRVAA
                     GRDFPPDVGDYKLIIQCGSCMLTRRETLARIQRAKQAGVPITNYGLCISCLQGVLERV
                     IEPFADAKAVFKNKGKGRGEEIC"
     gene            726..>1453
                     /locus_tag="PLY30_03980"
     CDS             726..>1453
                     /locus_tag="PLY30_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW65299.1"
                     /translation="MSTETAWVATRIKRDQIDKYLERGRDYIQDDRVWDEIKRAPEPS
                     ADRVREILAKSLEVKTLAPGELAELIRVKDPAMLEEMRETALRIKLKVYDNRIVTFAP
                     LYMGNYCVNNCVYCGFKRENLTQKRRVLSMDEIRRETESLAGRIGHKRIIAVYGEHPL
                     TGIDYITESMRTIYDVKVPTKHGYGNIRRINVNAAPMSTEELKHLHEVGLGTFQVFQE
                     TYHHETYAKLHPKGTLKSDYQWRA"
BASE COUNT          330 a          393 c          462 g          268 t
ORIGIN      
        1 cccgcgctcc ttggggacat catgccacgc ggggggctcg cggtactgat tatcccgatc
       61 gattttgagg cgcccaaggg acgcatcatc ctgccgcagg tgcaggcgat ccgggacgcg
      121 ctggaccacg gttccatgac cctcgtggtg cgggaaagtg aatacaaggc ggcgcttgcg
      181 cgtctgaaat cagtgccgga ccttgttgtc tgcgattccc aggtagtgga ccggatggtc
      241 gccgaaacgc cggcggaagt caaatgcacg acattctcga tcctcttctc ccgcctcaaa
      301 ggggatatcg tggaaatgac ccgtggcgcg ctggcgatca agaatctgag gccgggggac
      361 aaggtcctga tctccgaatc ctgcagtcat cacgcgattg acggggatat cgggcggatc
      421 aagattccgg cctggctccg gaaatacctc gggcatgacg tgacggttcg cgtcgcggcg
      481 ggtcgcgatt ttcctccgga tgtcggggat tacaagctca tcatccagtg cgggtcctgt
      541 atgctgacgc gccgggagac gctcgcccga atccagcgcg cgaaacaggc cggcgtgccc
      601 atcacaaact acgggctttg catctcttgt ctgcaggggg tactcgagcg ggtgatcgag
      661 ccttttgcgg atgcgaaggc agtgttcaaa aataaaggga aaggccgcgg agaggagatt
      721 tgctaatgag tacagaaacg gcgtgggtcg ccacacgcat caaacgggac cagatcgaca
      781 agtatctcga gcgcggccgg gactacatcc aggacgacag ggtctgggat gagatcaaac
      841 gtgcgccgga gccttccgcg gatcgtgtcc gggagatcct cgcaaagtcc ctcgaagtca
      901 aaacgcttgc gccgggagaa ctggccgagc tgatccgcgt caaagatccg gcgatgcttg
      961 aggaaatgag ggagacggct ctcaggatca agctgaaggt ctatgacaac cggatcgtga
     1021 cgttcgcccc gctttatatg ggcaattact gtgtcaacaa ctgcgtgtat tgcgggttca
     1081 agcgcgagaa cctgacgcag aagcggcgtg tgctttcgat ggatgaaatc cgtcgcgaga
     1141 cggaatcgct cgcaggaaga atcgggcaca aacggatcat cgcggtctac ggagaacatc
     1201 ctctgacggg gattgactac atcactgaaa gcatgcggac gatctatgac gtcaaggtgc
     1261 cgacgaaaca cggctacggg aatatccgca ggatcaacgt gaacgccgcg ccgatgtcga
     1321 ccgaggagct gaagcacctg cacgaggtcg gcctcgggac gttccaggtg tttcaggaga
     1381 cctatcatca cgagacctac gcgaagctgc atccaaaggg aacgctgaaa tcggattatc
     1441 agtggcgggc cta
//
LOCUS       DAOOBX010000255         1446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_97186_length_1446_cov_2.661395, whole genome shotgun sequence.
ACCESSION   DAOOBX010000255 DAOOBX010000000
VERSION     DAOOBX010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97186_length_1446_cov_2.661395"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..>1217
                     /gene="dxs"
                     /locus_tag="PLY30_03985"
     CDS             14..>1217
                     /gene="dxs"
                     /locus_tag="PLY30_03985"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPW65300.1"
                     /translation="MTTNWIQNINSPEDLKKVPLEKLPEVAAEYREFLIQSVAKTGGH
                     LGASLGTLELTIALHYVLNSPEDKICWDVGHQAYVHKMITGRRDRMTTMRQPGGLSGF
                     PAPWESKHDPFIVGHAGTAISEALGLACARDLKKGQEKVVALVGDGAITAGLSFEALN
                     HAGHLKKNMLVILNDNEMSIAKNVGAVSRYLNKVLTNPIYNRVRSELEKQLQKFPRLQ
                     KLANASLERMKHLFVPGILFEELGYRYFGPVDGHDVIRLVGILKDVFALGTCSLLHVI
                     TKKGRGCEFAEHDEEKGHGVVPFNVKTGEKIKSAPEKDAARSISFTQAFSDALLKLAR
                     RDPAVVAVTAAMPTGTGLHDFQKSFPDRFFDVGIAEQHAVTFAGAMAKCGLKPVVAVY
                     STFMQRFYD"
     assembly_gap    1218..1227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1228..>1446
                     /locus_tag="PLY30_03990"
     CDS             <1228..>1446
                     /locus_tag="PLY30_03990"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="catalyzes the formation of 1-deoxy-D-xylulose
                     5-phosphate from pyruvate and D-glyceraldehyde
                     3-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPW65301.1"
                     /translation="HDVALQRTGVVLALDRAGLVGADGATHQGIFDIAFLGSVPGIVI
                     GAPKDEFEMERMLDLGLGAPVPFGIRYPR"
BASE COUNT          311 a          401 c          442 g          282 t
ORIGIN      
        1 aaatggtcaa aggatgacga cgaactggat tcaaaatatc aatagtccgg aagatctgaa
       61 gaaggtgcct ctcgagaaac ttcccgaggt ggcggcggag tatcgcgaat tcctgattca
      121 gtcggtggcg aaaaccggcg gccatctcgg agcgagcctc ggcacgcttg aactcacgat
      181 cgcgcttcac tatgtgctca attcccccga agacaaaatc tgctgggatg tcggccacca
      241 ggcctatgtc cataaaatga tcacaggacg ccgtgaccgg atgacgacaa tgagacagcc
      301 cggcggcctg agcgggtttc cggccccttg ggaaagcaaa cacgatccgt tcatcgtcgg
      361 gcacgcgggg accgcgatct ccgaagcgct ggggctggcc tgcgcccggg acctcaaaaa
      421 gggccaggaa aaggttgtcg cgctcgtggg agacggggcg ataacggcgg gcctttcgtt
      481 cgaggccctc aatcacgcgg ggcatctcaa gaagaacatg ctcgtgatcc tgaacgacaa
      541 cgaaatgtcg atcgcgaaga acgtcggcgc ggtcagccgt tacctcaaca aggtcctcac
      601 gaatcccatc tataaccgtg tgcggagcga gcttgagaag caactccaga aatttccgag
      661 gcttcagaaa ctcgcgaacg cctctctgga gagaatgaag catctttttg ttcccggcat
      721 tctttttgaa gagctcggct accgctactt tggtcccgtg gacggccatg acgtgatccg
      781 gctggtcgga atcctgaaag acgtgttcgc gctgggcacc tgttctcttc ttcatgtgat
      841 cacgaaaaag ggacggggat gcgagttcgc ggagcatgat gaggagaaag gtcatggcgt
      901 ggtgcccttc aacgtcaaaa cgggagaaaa gatcaaaagc gcccccgaaa aagacgccgc
      961 ccgcagtatt tcgttcacgc aggccttttc ggacgcgctc ctgaagctcg cccgtcgcga
     1021 tccggcggtg gtcgcggtga ccgcagcgat gccaacggga acggggctcc atgatttcca
     1081 gaaatcgttc cccgaccgct ttttcgatgt cgggatcgcc gagcagcatg ccgtgacgtt
     1141 cgcgggcgcg atggcgaaat gcgggctgaa gcctgtcgtt gcggtttatt cgacattcat
     1201 gcagcgtttt tacgattnnn nnnnnnngca tgacgtggcg ctccagcgga ccggtgtcgt
     1261 gctcgcgctc gaccgggcgg gactcgtcgg cgcggacggc gcgacgcacc aggggatctt
     1321 tgatatcgcc tttctcgggt cggtgcccgg aatcgtgatc ggggcaccga aagacgaatt
     1381 tgagatggaa aggatgctcg atctcgggct cggagccccc gtgccttttg ggatccgcta
     1441 cccccg
//
LOCUS       DAOOBX010000256         1445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_97270_length_1445_cov_3.179137, whole genome shotgun sequence.
ACCESSION   DAOOBX010000256 DAOOBX010000000
VERSION     DAOOBX010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97270_length_1445_cov_3.179137"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1045)
                     /locus_tag="PLY30_03995"
     CDS             complement(125..1045)
                     /locus_tag="PLY30_03995"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPW65302.1"
                     /translation="MVSEVKGEAILETHLPLTRFAVGKVRDIYSIGSDKLLIVTTDRL
                     SAFDVVMPNPIPLKGKVLNNITLFWLDHFRDIIPQHLITADVDEMGLPENVVKDFRHE
                     IEGRSMLVKKARPLEIECVVRGYITGSGWKDYQRTGMVCGHKLLPGMKQCEKFPEPIF
                     TPSTKAKAGHDINIDLGEAIRIVGKDVAMRVRDLTIELYRKGQAFAASKGIIIADTKF
                     EFGIHDGKVMLIDEILTPDSSRFWPADRYETGHDQPSYDKQIVRNYLETLAWDKRPPG
                     PELPPDIIEKTTAAYCEIFKRLTGRDIRDI"
     gene            complement(1156..>1445)
                     /locus_tag="PLY30_04000"
     CDS             complement(1156..>1445)
                     /locus_tag="PLY30_04000"
                     /inference="COORDINATES: protein motif:HMM:NF021883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPW65303.1"
                     /translation="MLRNMEASRGMVFSQGLLLKLVEKGLTREDAYKLVQAAAKRIWE
                     DESANLRDVALSDKGILEHLSPAEVREAFSYDRHLRNVPAILKRAGILKNK"
BASE COUNT          255 a          450 c          397 g          343 t
ORIGIN      
        1 ctcgtatccc atcgtctcga gcgcgtgaag cgtcgtctgc tcctgcgggt ccagcaccga
       61 ttttttcaga cctggatgag tcccggcctt gaacttcatg aacgcaacct ttcccttctt
      121 taaattaaat atcgcgaatg tccctgcccg tcagtcgttt aaaaatctcg caataggccg
      181 cggtggtttt ttcgatgatg tcgggcggaa gttcgggtcc gggcggccgt ttgtcccacg
      241 ccagcgtttc cagatagttc cgaactatct gtttgtcgta gctcggctgg tcgtgccccg
      301 tctcgtacct gtcggcgggc cagaagcgcg agctgtcggg cgtcaggatt tcgtcgatca
      361 gcatgacctt cccgtcgtgg attccgaact cgaatttcgt gtcggcaatg ataatcccct
      421 tcgacgcggc gaacgcctgt cccttcctgt agagctcgat cgtgaggtcg cgcacccgca
      481 tcgcgacatc tttgcccaca atgcggatcg cctcgccgag atcgatattg atgtcatggc
      541 ccgccttggc cttcgtcgac ggcgtgaaga tcggctccgg gaatttttcg cactgtttca
      601 tgccgggcag aagcttgtgg ccgcagacca taccggtccg ctgataatcc ttccagccgg
      661 aacccgtgat gtaaccgcgc acgacgcatt cgatttcgag cggccgggct tttttgacga
      721 gcatcgaacg cccctcgatt tcatggcgga aatccttcac cacgttctcc ggaaggccca
      781 tctcgtcgac atccgcggtg atgaggtgct gcgggatgat atccctgaaa tggtcgagcc
      841 agaaaagcgt gatgttattg aggaccttgc ccttgagcgg gatcgggttc ggcatcacga
      901 catcgaacgc gctcaggcgg tcggtcgtaa cgatgaggag tttgtcgctg ccgatgctgt
      961 agatgtcacg gactttaccg accgcgaagc gcgtcagcgg cagatgagtt tccaggatcg
     1021 cttcgccctt cacttcgctc accacactct cccccctcct ctacccgtta tgcgtccgga
     1081 gcgcgggctt catggcttct tgcccggcat ggatgccttc cccatggctc cgaaccccgg
     1141 aatcaggatt ccgaactact tgtttttcaa aatacccgcc cgcttcagga tcgccggcac
     1201 attccgcagg tgacggtcgt agctgaacgc ctcccggacc tctgcgggcg aaagatgttc
     1261 gaggatcccc ttgtcgctca gcgcgacgtc ccggagattc gccgattcat cctcccagat
     1321 ccgtttcgcg gcggcctgga caagcttgta ggcatcctcg cgcgtgagcc ccttttcgac
     1381 gagcttgagg agcagcccct gggaaaagac catgccacgg ctcgcttcca tgttcctgag
     1441 catgc
//
LOCUS       DAOOBX010000257         1431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_98606_length_1431_cov_4.027616, whole genome shotgun sequence.
ACCESSION   DAOOBX010000257 DAOOBX010000000
VERSION     DAOOBX010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98606_length_1431_cov_4.027616"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /gene="atpD"
                     /locus_tag="PLY30_04005"
     CDS             <1..1179
                     /gene="atpD"
                     /locus_tag="PLY30_04005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPW65304.1"
                     /translation="GPISVPVGEQTLGRVFNLLGDAIDEAGAIREPGKKRSIHRKPPT
                     FEDLLPVTSILETGIKVIDLLAPYPKGGKVGLFGGAGVGKTVIVMELIHSIATEHGGV
                     SVFCGVGERTREGNDLWLELKESGVIKKTAMVFGQMNEPPGARLRVALTGLTMAEYFR
                     DEAHQDVLVFIDNIFRFTQAGAEVSALLGRMPSAVGYQPNLATEMAELQERIASTRAG
                     SITSVQAIYVPADDITDPAPAAAFSHLDGVTVLSRQIVELGIYPAVDPLASTSRMLDP
                     RIVGEDHYHTAREVQRILQRYKDLRDIIAILGMNELSQEDKELVTRARKLEKFLSQPF
                     FVAEAFTGRQGRYVQLKDTIASFKKIIAGELDHIPEQCFYMCGGIEDVIENFEKTKNR
                     "
BASE COUNT          299 a          449 c          426 g          257 t
ORIGIN      
        1 ggcccgatct cggtgccggt cggcgagcaa acactcggcc gcgtgttcaa cctgctcggg
       61 gacgcgatcg acgaagccgg cgcgatcagg gagcccggca agaaacgttc cattcaccgg
      121 aaaccgccca cgttcgaaga ccttcttccg gtcacttcga ttctcgagac cggcatcaag
      181 gtcatcgatc ttctcgctcc gtacccgaag ggcggcaagg tcggattgtt cggcggcgca
      241 ggcgtcggca aaaccgtcat cgtcatggaa ctcatccact cgatcgcgac cgaacacggc
      301 ggcgtctcgg tcttctgcgg tgtcggggag cgcacccgtg aagggaatga tctctggctc
      361 gagctgaagg aatcgggcgt catcaagaag accgcgatgg tgttcggcca gatgaacgag
      421 ccgcccggag cgcgcctgcg cgtcgccctc acgggcctga cgatggcgga gtacttccgg
      481 gacgaagcgc accaggacgt gcttgtcttc atcgacaaca ttttccgttt cacgcaggcg
      541 ggcgccgaag tatcggcgct cctcggccgc atgccgtcgg cggtcggtta ccagccgaac
      601 ctcgcgaccg aaatggcgga actccaggaa cgcatcgcgt ccaccagggc cggctccatc
      661 acgtcggtgc aggccattta cgttccggcc gacgacatca cggatccggc gcccgcagcg
      721 gccttctcgc atcttgacgg agtcacggtg ctctcgcgtc agatcgtgga actcggcatc
      781 tacccggccg tcgatcccct cgcatcaacc tcgcgcatgc tcgatccgcg catcgtgggc
      841 gaggaccact accataccgc gcgcgaggtc cagaggattc tccagcgcta taaggacctc
      901 cgcgacatca tcgcgattct tgggatgaac gaactttccc aggaagacaa ggagctcgtc
      961 acgcgcgcga gaaaactcga gaaattcctg tcgcagccat tcttcgtggc ggaagcgttc
     1021 acgggccgtc agggccgtta cgtgcaactg aaggacacga tcgcgagctt caagaagatc
     1081 atcgcgggcg agctcgatca tatccccgaa cagtgttttt acatgtgtgg cgggatcgag
     1141 gacgtgatcg aaaactttga aaaaaccaag aaccgataaa ctcaaaagga ctcgttcctt
     1201 gggacgagga atcaaaacgg atcccggagc gccggttccg ggtcccggaa tttaaaaatg
     1261 actgaacctg tttatacatt acggatcgtg accccggtgg gcatcgccta tgaaggttcc
     1321 gccgcgcaca ccatcgtgcc ggtcgagcgc ggcttcgtgg gcatcctcgc ccatcacgcg
     1381 ccgtacctga cctcgtccgc gggaggaaag ctctccgtca gggagaagaa c
//
LOCUS       DAOOBX010000258         1418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_99899_length_1418_cov_1.779164, whole genome shotgun sequence.
ACCESSION   DAOOBX010000258 DAOOBX010000000
VERSION     DAOOBX010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99899_length_1418_cov_1.779164"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /gene="leuS"
                     /locus_tag="PLY30_04010"
     CDS             complement(<1..1374)
                     /gene="leuS"
                     /locus_tag="PLY30_04010"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPW65305.1"
                     /translation="MASGFKAYPFDEIEQKWQAYWLAHKTFRTPDIGEGRGDRPKYYI
                     LDMFPYPSGSGLHVGHPEGYTATDIIARYKRMRGFNVLHPMGWDAFGLPAEQYAIDTG
                     THPAITTRKNIDTFRRQIQMLGFSYDWDREVDTTDPSYFKWTQWIFLQLYKKGLAYES
                     EVPVWWCDGCKAVLANEEVRSDGTCDRKGHKEVFRRPLRQWVLKITAYAERLLEDLKL
                     VDWSEAIKEMQRNWIGRSEGAEVDFVIADGAAKDEKIRVFTTRPDTLFGATYMVLAPE
                     HPLVDAITAPPHRDEVHQYRLGASRKSELERTQLEKDKTGVFTGAYAVNPVNGEKIPV
                     WIADYVMMGYGTGAIMAVPAHDTRDFEFATRFNLPVLQVVQPPDPGKDWRGFVGDGTS
                     VNSDGPEVSITGLETPDAKIKITKWLSEKGLGKASVQYKLRDWLFSRQRYWGEPIPII
                     HCPACGTI"
BASE COUNT          267 a          440 c          415 g          296 t
ORIGIN      
        1 aattgtcccg catgcgggac aatgtatgat cgggatcggt tcaccccagt aacgctggcg
       61 ggagaaaagc cagtcgcgaa gcttgtattg gacactcgcc ttcccgagtc ctttttccga
      121 aagccatttc gtgatcttta ttttggcgtc cggcgtctcg agacccgtga tcgagacctc
      181 gggcccgtcc gaattcacgg acgtcccgtc acccacgaac ccccgccagt ccttcccggg
      241 gtccggaggc tgcaccacct ggaggaccgg caggttgaac ctggtcgcga actcgaaatc
      301 gcgcgtgtcg tgcgccggca ccgccatgat cgcgcccgtc ccatacccca tcatcacgta
      361 atccgcgatc cagaccggga tcttctcccc gttcacggga ttgaccgcgt aagcgccggt
      421 gaaaacgccg gtcttgtcct tctcgagctg cgtgcgttcg agttcgcttt tcctggaggc
      481 ccccagcctg tactgatgga cctcgtcgcg gtggggcggc gccgtaatcg cgtcgacaag
      541 cggatgctcg ggcgcgagga ccatgtacgt cgccccgaaa agcgtgtcgg gacgcgtcgt
      601 aaaaacgcgg attttttcgt ctttcgccgc gccgtccgcg atcacgaaat ccacttcagc
      661 gccctcgctc cggccaatcc agttgcgttg catttccttg atcgcctccg accagtcaac
      721 gagcttcagg tcctcgagga gtctttcggc gtacgccgtg atcttcagga cccattgccg
      781 gagcgggcgg cggaagacct ccttgtgccc tttgcggtcg caggtaccgt cggagcggac
      841 ctcctcgttg gcgagcacgg ccttgcaacc gtcgcaccac cacaccggga cctcggactc
      901 atacgcgaga cctttcttgt agagctgaag gaagatccac tgggtccact tgaaatagct
      961 cgggtccgtc gtatcgacct cgcggtccca gtcgtagctg aaaccgagca tctgaatctg
     1021 gcggcggaag gtatcgatgt tctttctggt cgtgatagcc ggatgcgttc cggtatcgat
     1081 cgcgtactgt tccgcgggaa gaccgaacgc gtcccagccc atcgggtgca ggacgttgaa
     1141 cccccgcatg cgtttataac gggcgatgat atccgtggcg gtatagcctt cgggatggcc
     1201 gacatggagc cccgaaccgg aggggtacgg aaacatatcg aggatgtaat acttgggccg
     1261 gtcgccccgg ccctcgccga tatcgggagt gcggaaggtc ttgtgcgcaa gccaataggc
     1321 ctgccatttt tgctcgatct catcaaaggg atacgctttg aatccgctgg ccatagagaa
     1381 cctcaaaaaa tgcgagagga gggaatcgaa ccctcatg
//
LOCUS       DAOOBX010000259         1407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_100958_length_1407_cov_3.182692, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000259 DAOOBX010000000
VERSION     DAOOBX010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100958_length_1407_cov_3.182692"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..336
                     /locus_tag="PLY30_04015"
     CDS             7..336
                     /locus_tag="PLY30_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPW65306.1"
                     /translation="MRAIALLTISNIFMTIAWYGHLRFKNAALWKVIVISWLIAFVEY
                     CFQVPANRIGSYTYSTAQLKTIQEIITLIVFCAFSVVYLKEGLKWNYLVGFAFIVLAA
                     FFIFKKW"
     gene            345..908
                     /locus_tag="PLY30_04020"
     CDS             345..908
                     /locus_tag="PLY30_04020"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPW65307.1"
                     /translation="MKRSLGAKTILFPAPVLIICSYDANGAPNAMNAAWGGIACSEPP
                     AITVSVREPRKTYENIMAQRDFTVCIPSEKYIREADYVGIVSGKKEDKFKATGLTAVK
                     ADKVNAPYIQEFPLTVECRLMQTVRLGSHTQFIGEVLDVKADESVLNAKGFPDVEKMK
                     AFMFDPATGSYYGAGKFLGQAFSIGKK"
     gene            913..1284
                     /gene="crcB"
                     /locus_tag="PLY30_04025"
     CDS             913..1284
                     /gene="crcB"
                     /locus_tag="PLY30_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HPW65308.1"
                     /translation="MKWIALVSGGTLGTLARYWFGGAVYQTLGASFPYGTLVVNLTGC
                     WLIGLLTVLAEEKFLLGPSARVFLMIGFCGAYTTFSTFMLETADLMKSGENFRAFMNV
                     FPSVVAGFLALRAGILTAKFF"
BASE COUNT          300 a          416 c          391 g          300 t
ORIGIN      
        1 cgggtcatga gagcgatcgc ccttctcacc atttccaaca tcttcatgac catcgcctgg
       61 tacggtcatc tgcgcttcaa gaacgcggct ctctggaagg tcatcgtgat tagctggctg
      121 atcgccttcg tcgagtattg ttttcaggtg ccggccaacc ggatcggctc ttacacgtac
      181 tcgacggccc agctcaagac gatccaggag atcatcaccc tgattgtttt ctgcgcgttc
      241 tcggtcgtat acctcaaaga aggtctcaaa tggaattatc tcgtcggctt cgcgttcatc
      301 gtgctggccg cattcttcat cttcaaaaaa tggtgaggtg aaccatgaaa agatcccttg
      361 gcgccaaaac aatccttttc ccggctccgg tcctgatcat ctgctcttac gacgcgaacg
      421 gcgcgccgaa cgccatgaac gcggcgtggg gcgggatcgc ctgttcggaa cctccggcga
      481 tcacggtctc cgtgcgggaa ccgcgaaaga cctacgaaaa catcatggcg caacgggact
      541 tcacagtgtg catcccttcc gaaaaataca tccgggaagc ggattatgtg ggtatcgtct
      601 cgggaaaaaa agaggacaag ttcaaggcca ccggtctcac agccgtcaag gcggacaagg
      661 tcaacgcccc ctacatccag gaattccccc tcacggttga gtgccgcctg atgcagacgg
      721 tcaggctcgg aagccacacg cagttcatcg gcgaggtcct ggacgtgaag gccgacgaga
      781 gcgttttgaa cgcgaagggg tttccggacg tcgaaaaaat gaaggccttc atgttcgacc
      841 cggcgaccgg ttcctactac ggggccggaa aattcctggg gcaggccttc tcgatcggga
      901 agaaataacg ccatgaaatg gatcgccctg gtctccggcg ggacgctcgg aaccctcgcc
      961 cgttactggt tcggaggcgc ggtttatcaa acactcggcg cctcttttcc ctatggcacg
     1021 ctcgtcgtga acctcacggg atgctggctc atcggtcttc tcacggtcct cgcggaagag
     1081 aaattccttc tgggaccgtc ggcgcgtgtt ttcctgatga tcggtttctg cggcgcctac
     1141 acgaccttct cgaccttcat gctcgaaacc gcggacctga tgaaaagcgg cgaaaacttc
     1201 cgcgcgttca tgaacgtttt cccgagtgtc gtggccggtt ttctcgcgct gcgcgccgga
     1261 atcctgaccg caaaattctt ctaaacccct ccgggggaag agctcatcac cctcgagaag
     1321 gcaagcgcgg tcctttgccg cgcgggctaa ccgtggcctc tccggggaaa cagcggcacg
     1381 gcgggttgaa ataaaaaatg gaaacgg
//
LOCUS       DAOOBX010000260         1405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_101166_length_1405_cov_3.829630, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000260 DAOOBX010000000
VERSION     DAOOBX010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101166_length_1405_cov_3.829630"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLY30_04030"
     CDS             <1..638
                     /locus_tag="PLY30_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPW65309.1"
                     /translation="LDIFLMHPEGYHTYWNSAVRPGYSGVALFTRERPLKVTNGFGIK
                     RFDDEGRVITAEYPEFAFLTVYFPNGKASDERLRYKMDFYEETLEFVRKLKKKQPNII
                     VCGDYNTAHKPIDLARPKENEDVSGFLPVERAWLDKWVADGQIDVFRKFHEGPGHYSW
                     WDLKSGARERNVGWRIDYHFVTETLVPKVKSATILKDVAGSDHAPVEIQLK"
     gene            723..1163
                     /locus_tag="PLY30_04035"
     CDS             723..1163
                     /locus_tag="PLY30_04035"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPW65310.1"
                     /translation="MNSLIKSVLINASLEKVWALMADTRRTPEWVDGVQESERTGEVS
                     EGKGLCWRERSLFKNQNIEMEHEFIEWEPMSRARIKTSLPMNGWMDRTLEFSRTPDGV
                     EVKINAKWDLGIAGMLLGERKVFEILETSFEQTLLNWKERVEKA"
BASE COUNT          322 a          357 c          432 g          294 t
ORIGIN      
        1 agctcgacat ctttctcatg caccccgagg gttatcacac ctactggaat tccgcggtgc
       61 ggcccgggta cagcggcgtc gctcttttta cgcgtgagcg tccgctcaag gtgacgaacg
      121 ggttcgggat caagcgattc gatgacgaag gccgcgtgat tacggccgaa tatccggagt
      181 tcgcgttcct cacggtttat ttccccaacg ggaaagcaag cgacgaacgg cttcggtaca
      241 agatggattt ttacgaagag acgcttgagt tcgtccgcaa gctcaagaaa aaacagccca
      301 acattatcgt ttgcggtgac tacaataccg cgcataagcc aatcgatctc gcgcggccga
      361 aggagaatga ggacgtttcg ggattccttc cggttgaacg ggcctggctc gacaaatggg
      421 tcgcggacgg acagatcgat gttttccgga agtttcatga aggacccggg cactattcgt
      481 ggtgggacct gaaatcaggg gcgcgggagc ggaatgtcgg atggcggatc gattatcact
      541 ttgtgactga aactcttgtg cccaaagtaa aatcggcgac gatcctgaag gatgtggcgg
      601 ggtcggatca tgcgccggtg gagatacaat taaaatagga cacgaagccg cgcgccgcgc
      661 accacacgaa agattttccg tcctgtggcg ttttgcgccg ttgtggcctt gtggcggagg
      721 ctatgaactc gctcattaaa tctgttttaa tcaacgcttc gctcgaaaag gtctgggctc
      781 tcatggccga tacgcggcgc acgcccgaat gggtcgatgg cgtccaggaa agcgagcgga
      841 cgggggaggt ctcggagggt aaggggcttt gctggcggga gcgttccctt ttcaagaacc
      901 agaacatcga aatggaacat gagtttatcg agtgggagcc gatgtcgcgt gcgcgcatca
      961 agacatcgct tcccatgaac ggatggatgg accgcacctt ggaattctcc cgaacgccgg
     1021 acggcgtcga agtgaagatc aacgcaaaat gggacctcgg gatcgcgggg atgctgctcg
     1081 gcgagcggaa ggtttttgag atcctcgaga cgagcttcga gcagacactt ctgaactgga
     1141 aagaaagagt cgagaaagca taatcgccac aagccgcatg ttacgtgaaa agcttttcgt
     1201 cttgtggcgt gtacctctgt ggccggagat caggccgctt gccccgtagc ctgccgggac
     1261 aggatttccg ccgtcagacc ttccagaagt ttcaggaatt cagcgttctt cgccgccctg
     1321 tagagttccg cgttcagggc gatcagccgg tacagaacca gcgagacggg ggactccggc
     1381 aacacaacga atgtcgggtt ctcat
//
LOCUS       DAOOBX010000261         1400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_101763_length_1400_cov_2.049071, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000261 DAOOBX010000000
VERSION     DAOOBX010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101763_length_1400_cov_2.049071"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /gene="thiF"
                     /locus_tag="PLY30_04040"
     CDS             complement(<1..484)
                     /gene="thiF"
                     /locus_tag="PLY30_04040"
                     /inference="COORDINATES: protein motif:HMM:NF006395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HPW65311.1"
                     /translation="MNPEAFERDLIAKLGAEGFRRMRAAKIGIAGAGGLGSNCATALV
                     RSGFRDLVIADFDVIDDTNLDRQAYFAEQVGRPKVEALAENLHRTLPGLTLHALVLKL
                     DRSNLPSVFAECDAVVECFDQADAKSMFVETLLPTGKFLVTASGLGGIGASDEIRIHR
                     I"
     gene            complement(481..>1400)
                     /gene="thiH"
                     /locus_tag="PLY30_04045"
     CDS             complement(481..>1400)
                     /gene="thiH"
                     /locus_tag="PLY30_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HPW65312.1"
                     /translation="RQFGRVIQLYTPMYLSNFCENQCEYCGFNATNRLPRRTMTLEEV
                     EREAAYIASTGLEHILILTGDARKRAPVSYIRDAVQVLRKHFSSISVEVYALTEAEYA
                     ELAAEGVDGLTIYQETYDEAVYDRVHRSGPKKDFRFRLEAPERAARSGMRSVNIGALL
                     GLSDWRVEAFRLGLHARYLQDTFPDVEVGASVPRLRPHEGDFRGDEKISDRDFVQIIL
                     AMRLFLSRLGLTLSTRESSELREHLVPLGITKMSAGSTTEVGGHTLATKTDRSAPQFR
                     IADERDVEAIKAMLAAKGYQPVLKDWMPI"
BASE COUNT          262 a          451 c          420 g          267 t
ORIGIN      
        1 tgatgcgatg gatgcgaatc tcgtcgctgg cgccgatccc cccgagtccc gaagcggtca
       61 cgaggaattt tcccgttggc agcagcgttt cgacgaacat gcttttcgcg tcggcctggt
      121 cgaaacattc cacgacggcg tcgcattccg cgaacacgga agggagattc gagcggtcga
      181 gcttcaggac gagcgcgtga agggtcaggc ccggaagcgt tcggtgaagg ttctcggcca
      241 gcgcctcaac cttcggcctc ccgacctgct ccgcgaaata agcctgccgg tccaggttcg
      301 tgtcgtcgat cacgtcaaaa tccgcgatca caaggtcccg gaaccccgaa cgcacgagag
      361 cggtcgcgca attcgagccg agcccgcccg cgcccgcgat cccgatcttc gcggcccgca
      421 tccgccggaa accttcagcg ccgagcttcg cgatcaggtc cctttcgaag gcttcgggat
      481 tcatatcggc atccagtcct tcaggacagg ttgataacct ttcgccgcga gcatggcctt
      541 gatcgcctcg acatcgcgtt cgtccgcgat ccggaactgc ggcgcgctcc ggtcagtttt
      601 tgtcgcgagc gtgtgcccgc ccacttcggt agtggaaccc gccgacattt tcgtgatccc
      661 cagaggaacg aggtgttccc ggagttccga cgattcccgc gtcgagagcg tgagcccgag
      721 gcgcgacagg aaaagccgca tcgcgaggat gatctggacg aagtcccggt cgctgatctt
      781 ttcatctccc cggaaatcgc cttcgtgggg cctgagacgc ggcaccgagg cgccgacctc
      841 cacgtccggg aaagtgtcct gcaggtaccg agcgtggagc ccgagacgaa aggcctcgac
      901 gcgccaatcg ctcagaccga gtaatgcccc gatattcaca gaccgcattc cgctcctcgc
      961 ggcccgttcg ggggcctcga gacggaaccg gaaatctttc ttcggcccgg accggtggac
     1021 gcggtcatag acggcttcgt cgtaggtctc ctggtagatc gtgagaccgt ccaccccttc
     1081 ggcggccagt tcggcatact cggcttccgt gagcgcgtag acctcgaccg agatcgaact
     1141 gaaatgtttc ctgaggactt ggaccgcgtc cctgatataa gagaccggtg cccgcttgcg
     1201 ggcatctccc gtcagaataa ggatatgttc cagaccggtc gaagcgatgt acgcggcttc
     1261 gcgctccact tcctcaaggg tcatggtccg ccgcggcaaa cggttcgtcg cgttgaaccc
     1321 gcaatactcg cactggttct cgcagaagtt cgaaaggtac atcggcgtgt aaagctggat
     1381 gacccgtccg aactgccgga
//
LOCUS       DAOOBX010000262         1387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_103045_length_1387_cov_4.306306, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000262 DAOOBX010000000
VERSION     DAOOBX010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103045_length_1387_cov_4.306306"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..615
                     /locus_tag="PLY30_04050"
     CDS             148..615
                     /locus_tag="PLY30_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPW65313.1"
                     /translation="MLKFTLSEFKEGVRVPVAGEYDPKQIEVEFPDLHYVAPVRLEGF
                     AEKTAGTFRFEGTLTTRARKTCGRCLKEMESGFSEDFDWIYETENRETIDPADNVREL
                     LIIDHELAYLCQENCKGLCPVCGKNLNENTCKCRQSSYHSFPVIKKLKPKKES"
     gene            617..817
                     /gene="rpmF"
                     /locus_tag="PLY30_04055"
     CDS             617..817
                     /gene="rpmF"
                     /locus_tag="PLY30_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPW65314.1"
                     /translation="MANPKRRHSNTRTRLRRANDALKLKSASRCPQCRAFVLPHRVCG
                     ACGYYKGKQVKTMKVKSKEKAA"
     gene            817..>1387
                     /locus_tag="PLY30_04060"
     CDS             817..>1387
                     /locus_tag="PLY30_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPW65315.1"
                     /translation="MIRIAVDGMGGDNGPAVVVEGAVQAANDFEHLEILLVGPTDILK
                     AELNRHKVLGGKITIVEAPEVVGMGESPVAALKKKKNSSIKISIDLLHKKEVDAAISA
                     GNTGAMVAASTLTLGMLPGVKRPGIAITIPTMHGMCCTLDVGANLNPSAFELFQYAIM
                     ADVFARNIIRKKRPSIGLLNIGEEESKGTE"
BASE COUNT          333 a          401 c          383 g          270 t
ORIGIN      
        1 acaccgcctt cctgatgccg tccgaacact atttcttcct ttcgtcgcgc gtcgccaagg
       61 agatcgcgca gctccggggc gatgtcacgg cctatgtccc gaaattcgtc gcgaggaaac
      121 tcgctgaaaa attcagaggc tgacaccatg ctgaaattca cgctgtccga attcaaggaa
      181 ggggttcgcg tccccgtcgc gggggagtac gaccccaagc agattgaggt cgaattcccg
      241 gacctccatt acgttgcgcc ggtccgtctc gaaggattcg ccgaaaaaac cgcgggcact
      301 ttccgtttcg agggtaccct gaccacgcgc gcgcgcaaaa cctgcggccg gtgtctcaaa
      361 gagatggaat ccggtttcag tgaggatttt gactggatct atgagaccga gaaccgggag
      421 acgatcgatc ccgcggacaa tgttcgtgag ctgctaatca tcgatcacga actcgcgtac
      481 ctttgtcaag aaaattgtaa gggactttgt ccggtttgcg gtaaaaacct caatgaaaat
      541 acttgcaaat gccgacaatc cagttatcat tcatttcctg ttatcaagaa attgaagccg
      601 aagaaggagt cttgatatgg ccaatccaaa acggagacat tccaacacgc gcacacggct
      661 gcgccgggca aatgacgcac tgaaactcaa gtccgcgtcg cgatgccccc agtgccgcgc
      721 gttcgtcctg ccgcaccgcg tctgcggcgc ctgcggttat tacaaaggca agcaggtcaa
      781 gacgatgaag gtcaagtcga aggaaaaggc cgcctgatga tccgcatcgc cgtcgacgga
      841 atgggcgggg ataacggacc cgcggtcgtc gtggaaggtg ccgtccaggc cgccaacgat
      901 tttgaacacc tcgagattct cctcgtcggc cccaccgaca ttctgaaggc cgagttgaac
      961 cgtcacaagg tcctcggagg gaagatcaca atcgtcgagg cgcccgaagt ggtggggatg
     1021 ggggagtctc ccgttgccgc cctcaaaaaa aagaagaatt cgtcgatcaa gatttcgatc
     1081 gacctcctgc acaaaaaaga ggtcgatgcc gccatttccg ccggcaatac cggcgctatg
     1141 gtcgcggcgt ccacgctgac ccttggcatg cttcccggag tcaaacggcc cggaatcgcg
     1201 atcacgatcc cgacgatgca cggtatgtgc tgtaccctcg atgtcggcgc gaacctgaac
     1261 ccgtccgcgt tcgagctttt tcagtacgcc atcatggcgg acgtcttcgc cagaaacatc
     1321 atcaggaaaa agcgaccttc gatcggcctc ctcaatatcg gcgaagagga atccaaaggg
     1381 accgagg
//
LOCUS       DAOOBX010000263         1381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_103743_length_1381_cov_2.213424, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000263 DAOOBX010000000
VERSION     DAOOBX010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103743_length_1381_cov_2.213424"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLY30_04065"
     CDS             <1..224
                     /locus_tag="PLY30_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPW65316.1"
                     /translation="PKILIDLSKVDYMDSSGIATFVEAFQRTKRYSGRLILAGLTAAV
                     RGVFEIAKLENIFEIAADRGEAQSRFSGK"
     gene            246..1046
                     /locus_tag="PLY30_04070"
     CDS             246..1046
                     /locus_tag="PLY30_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW65317.1"
                     /translation="MKETCGRIGRATFFVMAGVARLWKLGLVVGRFFLTSFVRPRIVP
                     RESFFRQMVLMGIDSTAIVSLVGASVGMVLALQAAYSLRTFGAVMYTGSLVGVAMARE
                     LGPLIAAIVICGRIGARMAAEIGTMMVQEEVDALTTMAINPVRYLVLPRVMALAIMLP
                     CLTVIADIMGMIGGYLIGITGLGINSYVYISKTVDALVLKDLYTGLVKSFIFSVLIAL
                     VSCHQGLSVSGGADAVGKATTQAVVISIVLIIVVDCLATAVIYYALPT"
     gene            1055..>1381
                     /locus_tag="PLY30_04075"
     CDS             1055..>1381
                     /locus_tag="PLY30_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW65318.1"
                     /translation="MADPIIEIKELVKRFGDRTVLDGVNVVIPEGKITVIMGGSGCGK
                     STLLRHLIGLHQPTSGEVWVDGRDIAGLDEKGMNEVRKKFGMLFQGSALFNSMTVGEN
                     VAVPLKE"
BASE COUNT          260 a          356 c          432 g          333 t
ORIGIN      
        1 caccaaagat cttgatcgat ctttcgaaag tcgattatat ggacagttcc ggcatcgcga
       61 ccttcgtgga agcctttcag aggacgaagc gttatagcgg tcgactgatc ctggcggggc
      121 ttaccgcggc ggtgcgcggg gtcttcgaga tcgcgaagct cgaaaatatt ttcgagatcg
      181 cggccgatcg cggtgaagct cagagccgct tttccggcaa ataaccagca aaaggaacct
      241 ggcggatgaa agaaacctgc gggcggatcg gacgtgcgac tttttttgtc atggccggtg
      301 tcgcgcggtt gtggaaactt ggccttgtgg tggggcgttt ttttctgacc tcgttcgtcc
      361 gccccaggat tgtcccgagg gaatcctttt tccgtcaaat ggtcctcatg gggatcgatt
      421 ccacggcgat tgtttcactc gtcggcgcct ctgtcgggat ggtgcttgcg ttgcaggcgg
      481 cctattctct caggaccttc ggtgcggtca tgtacacggg gagtcttgtc ggcgtggcga
      541 tggcccgtga actcgggccc cttatcgcgg cgatcgtgat ctgcgggcgc atcggcgcgc
      601 gtatggccgc ggagatcggg accatgatgg tccaggaaga ggttgatgcg cttacgacga
      661 tggcgatcaa tccggtccgc tatcttgtcc tgccgcgcgt gatggccctc gcgatcatgt
      721 tgccgtgctt gacggtgatc gcggatatca tgggaatgat cggcgggtat ctgatcggca
      781 tcacggggct cggcatcaat tcctatgtct atatcagcaa gaccgtcgac gctctcgttc
      841 tgaaggatct ctacaccggc cttgtgaaaa gcttcatctt cagcgtcctg atcgcgcttg
      901 taagttgtca ccagggtctt tcggtctcgg gcggcgcgga cgcggtggga aaagccacca
      961 cgcaggctgt ggtcatttcg atcgttctca tcatcgtggt cgactgtctc gcgaccgcgg
     1021 tcatctatta cgcgcttccc acctgaggtg aactgtggcg gatcccatca ttgagatcaa
     1081 ggagctggtg aaacggttcg gtgaccggac cgttctggac ggggtcaatg tcgtgatccc
     1141 ggaagggaag atcacggtta ttatgggagg atccggttgc ggaaaaagca cgcttctgcg
     1201 ccacctgatc ggactccacc agccgacctc gggagaagtt tgggtcgacg gcagggacat
     1261 tgcgggtctt gatgaaaaag gcatgaacga ggtccgtaaa aaattcggaa tgctcttcca
     1321 gggttccgcg ctttttaatt caatgaccgt cggcgagaat gttgccgttc cgcttaagga
     1381 a
//
LOCUS       DAOOBX010000264         1378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_104030_length_1378_cov_2.585034, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000264 DAOOBX010000000
VERSION     DAOOBX010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104030_length_1378_cov_2.585034"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PLY30_04080"
     CDS             <1..1358
                     /locus_tag="PLY30_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW65319.1"
                     /translation="FTIQVAPEDCTGCGLCVETCPAKSKTDPEKKAINMASQQPLREP
                     EKVNWAYFQSIPDFDRTKLNTGMINQQQAMRPLFEFSGACGGCGETPYVKLISQLFGD
                     RAVIANATGCSSIYGGNLPTTPWAKDANGRGPAWANSLFEDCAEFGYGFRMAIDKQKE
                     FACELLLRLGDKVGADLAKALIEAPQKTEADIQAQRGRVAELKKKLASVSPDPDAAQL
                     LSLADVLVKRSVWSFGGDGWAYDIGFGGLDHVLASGKNVNVLVLDTEVYSNTGGQASK
                     STPRSAVAKFAMGGKASGKKNLGLIAMTYGNVYVASIAMGANDQQALKAILEAEAYDG
                     PSLVIAYSHCIAHGINMTTGMQGQKAAVASGQWLLYRFDPTKLERGENPLTLDSKAPS
                     APVEQFMMMENRFKMLMKSRPEHAKELFKLAQKDADYRWKLFEYLAQGKPQPEAPKVT
                     A"
BASE COUNT          300 a          388 c          429 g          261 t
ORIGIN      
        1 agttcacgat tcaggtcgcg ccggaagatt gcaccggctg cggcctctgc gtggaaacgt
       61 gtcctgccaa gagcaagact gatcccgaga aaaaagccat caacatggcc tcccagcagc
      121 cgctccgcga accggagaag gtgaattggg cgtatttcca gagcattccc gattttgatc
      181 gcacgaagct gaataccggg atgatcaacc agcagcaggc catgcgaccc ttgtttgaat
      241 tttcgggcgc ctgcggcggt tgcggcgaga cgccgtatgt gaaactgatc tcgcagcttt
      301 tcggtgaccg cgccgtcatt gccaacgcaa ccggctgctc ctcgatctac ggcgggaacc
      361 ttccgaccac accgtgggcc aaggatgcca acggccgcgg gccggcttgg gccaattcgc
      421 tgtttgagga ttgcgcggag ttcggctacg ggttccgtat ggcgatcgac aaacagaaag
      481 aatttgcctg tgaacttttg ttaagacttg gcgacaaggt cggagccgac ctcgcgaagg
      541 cactgatcga agccccgcag aagaccgagg cggacatcca ggcccagcgc gggcgtgtcg
      601 cggagctcaa gaaaaaactt gcttccgtta gcccggatcc cgatgccgcc cagctgcttt
      661 cgctggcgga tgtgctcgtc aaaaggagcg tctggagttt cggcggcgac ggttgggcct
      721 atgatatcgg gttcggcggt ctggaccacg tgctcgcttc cggaaagaac gtgaatgtcc
      781 tcgtgctgga tacggaagtt tattccaata cgggcggcca ggcatcgaag tccacgccgc
      841 gttccgcggt tgcgaagttc gcgatgggcg gaaaggcgtc cgggaagaag aatcttggct
      901 tgatcgccat gacctacggg aacgtttatg tcgcgtcgat cgcgatgggt gccaacgacc
      961 agcaggcgct caaggcgatc ctcgaggccg aagcgtatga cgggccttcc ctcgtgattg
     1021 cctacagcca ttgcatcgcg cacgggatca acatgacgac ggggatgcag ggccagaagg
     1081 ccgccgtggc ttccggccag tggctgctct atcgctttga tccgaccaag cttgagagag
     1141 gggaaaaccc gctcacgctc gattcgaagg cgccgtcggc accggtcgaa cagtttatga
     1201 tgatggaaaa ccgcttcaag atgctgatga aaagcaggcc ggaacacgcg aaggagcttt
     1261 tcaagctcgc ccaaaaggac gcggattacc ggtggaagct tttcgagtat ctcgcgcagg
     1321 ggaaacccca gccggaggcg cccaaggtta ccgcgtaatc acatattaac cgagtccg
//
LOCUS       DAOOBX010000265         1374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_104487_length_1374_cov_2.423806, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000265 DAOOBX010000000
VERSION     DAOOBX010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104487_length_1374_cov_2.423806"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>1374
                     /locus_tag="PLY30_04085"
     CDS             24..>1374
                     /locus_tag="PLY30_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65320.1"
                     /translation="MSDPVYDKDLEAARNLLRREKGNLSVMENNRKLLAANPKAYHPK
                     YREFKQLASLDNQIAARKALIKQLEADVQERETVPARSEWRTGEESPSPIYDEMLLWD
                     LKNGNHGRQREREAWFGRVANEPILRNFLASNLPELLNHQDPWVRDFSRKLLARINAM
                     PRRSEMRENAEAMVAGVAADLRDYIKGDVDFFLDAVGSRTNLTILKDEHSDIADVIAY
                     ALREAGLNDKQAESFYTKNHDAILRALEATRSRTRGDRVELPVNPMLAPPERSGAAVF
                     AIHPAFRLMPVDKRAVLPSKGTKAERTRGSAVLDLLKAIAAVGFFIGGIGLMGTLEFE
                     DEVRSESENIEWAKIYGITESQYREFAEEFARDPFAAGDVRSYILGKLGVEVTSATWN
                     GVIEGSGIDESEDAEAVRREVRMRSGLRNVPEKEGVITPGRAEMRGDLPRATDDELRF
                     "
BASE COUNT          323 a          352 c          417 g          282 t
ORIGIN      
        1 cccgtaaacg ggctgaggcg cgtatgtccg atcccgttta tgacaaggat ctcgaggccg
       61 cgcgtaatct tcttcgccgg gagaagggga acctttccgt catggaaaac aacaggaaac
      121 ttctggccgc gaacccgaaa gcctatcatc ccaagtatag ggaattcaaa caactggcgt
      181 ctctcgataa tcagatcgcc gcccggaagg ccctgatcaa acaactcgag gcggatgtgc
      241 aagaacggga aactgtgccc gcgcgctcgg aatggcgcac tggtgaagaa tcgcccagcc
      301 ccatttatga cgaaatgctt ctttgggatc tcaaaaacgg gaaccatgga aggcaacgtg
      361 agagggaagc ctggttcgga cgcgtggcga atgagccgat tctccggaac ttcctcgctt
      421 caaacctccc ggaattgctg aatcaccaag acccttgggt aagggatttt tcgcgcaagc
      481 ttcttgcgag aatcaacgcg atgccgcggc gttccgaaat gcgggaaaac gcggaagcta
      541 tggtcgctgg agtcgctgcg gatcttcgcg attatatcaa aggagatgtc gatttttttc
      601 tcgatgccgt agggagtcgg acgaacctaa cgatcctcaa ggacgagcat agcgatatcg
      661 ctgatgtgat cgcctacgcg cttcgcgaag caggtttgaa tgacaagcag gcggagagtt
      721 tttacacaaa gaatcacgac gcgattctga gggctctcga ggcgacgcgc tcccgaacac
      781 gcggtgaccg cgttgagctc cccgtgaatc cgatgctggc cccgcctgaa agatcaggcg
      841 cagctgtttt cgcgatccat cccgctttcc ggttaatgcc tgttgataag agagccgtct
      901 tacccagcaa ggggaccaag gctgaaagga cgaggggctc ggcggttctc gatttgctta
      961 aagcgatcgc agcggttgga tttttcatcg ggggcatcgg actcatgggg acattggaat
     1021 ttgaggatga agtgcgttcc gaaagtgaaa acatcgagtg ggcgaagatt tatgggatca
     1081 cagaatccca ataccgggag ttcgctgaag agttcgcccg ggatccgttt gcggcgggtg
     1141 atgtccgctc ctacatcctc ggtaaactcg gggttgaagt gacctccgcg acctggaatg
     1201 gcgtgatcga gggttccggg atcgacgaat ctgaagatgc cgaagctgtt cgccgggaag
     1261 tccgcatgcg ttccgggttg aggaatgttc ctgaaaaaga aggcgtgatt acgcccggcc
     1321 gcgccgaaat gcggggtgat ctgccgcggg cgaccgacga cgaactgcgc ttca
//
LOCUS       DAOOBX010000266         1372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_104663_length_1372_cov_3.218679, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000266 DAOOBX010000000
VERSION     DAOOBX010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104663_length_1372_cov_3.218679"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLY30_04090"
     CDS             <1..375
                     /locus_tag="PLY30_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016866616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW65321.1"
                     /translation="YHGHKRTYHVQVEGVPAGQTLAKLEKGVLIRRLKTAPCQARLMK
                     DPHYPERHPPIRFRLTVPTSWIEMRLIEGKNRQVRRMTAAVGHPTLRLIRVAIGQLHL
                     GKLPPGQWRELEQREIDLLLKN"
     gene            complement(609..>1372)
                     /locus_tag="PLY30_04095"
     CDS             complement(609..>1372)
                     /locus_tag="PLY30_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPW65322.1"
                     /translation="SGSTNMLKGSGVDEAWTTGVQLAEGVIELLKAGKPFTKENLETV
                     YLAKRRSSWVQKEGKIAEKARDGFSACFELGMMGMGLAGFTNGLINIPVKLKPTYARI
                     PSLEKYYGKKVPGEKIDAAFKEMASTNSSMHDAMMDASGWPKIRYDGKLLVSHQDALL
                     LGGKVQAAEGYADHVVFLDAGKCQKCRTKICIEMCSGQAITAGSDGGVPLFDREKCIH
                     CAACLWNCASAREEGSDLTNVDFRAGSGGLHSSIN"
BASE COUNT          290 a          431 c          372 g          279 t
ORIGIN      
        1 tatcacgggc acaagcgaac ctatcacgtc caggttgaag gcgttccggc gggacagaca
       61 ctcgcaaagc tggaaaaagg cgtgctgatc cggcggctga agaccgcacc ttgccaggcg
      121 cgtctgatga aggatcccca ttatcccgaa cggcaccccc cgatccgatt ccgtctgacc
      181 gtccccacaa gctggatcga gatgcggctg atcgaaggaa agaaccgtca ggtccggcgt
      241 atgaccgcgg ccgtcggtca ccccaccctg cgtctcatcc gcgtcgcgat cggacagctc
      301 catctcggga aactcccacc cggccaatgg cgcgaactcg aacagcgtga gattgatctc
      361 ctgctgaaga actgaatcct ttcagcctgc gcggttgaac aggtgcaacc gcgcgggggg
      421 atgcgggtac tctggccgcc cagccagcct ttggcgaatc gaaccgggac ttttttcccg
      481 ttatgaggtg tactgaatat gccggaggaa tccgaaagcg gcccggagat attcgtttta
      541 tccacgccgc ctggacctta cacacccgca aggcgtgaca aatcgcacct cgcaggtgtc
      601 ggggattgtt aattgatgct ggaatgcaga ccgccggaac ccgcgcggaa atccacattg
      661 gtcagatcag aaccttcttc acgcgcggag gcgcagttcc agagacaggc cgcgcagtgg
      721 atgcactttt cgcggtcaaa gagaggaacg ccgccgtcgg agccggccgt gatcgcctgg
      781 cccgagcaca tttcgataca aatcttcgtc cggcactttt gacatttccc cgcgtcgagg
      841 aacaccacgt gatcggcata accttccgca gcctggacct tgccgccgag aagcagcgcg
      901 tcctggtggg aaacaagaag cttcccgtca taccgaatct tcggccaacc cgacgcgtcc
      961 atcatggcgt cgtgcatcga ggaattggtc gatgccattt ccttaaaggc cgcatcgatc
     1021 ttttcccccg gaaccttctt gccgtaatat ttttcaagcg aaggaattcg cgcgtaagtc
     1081 ggtttcagct tcacgggaat attgatcaga ccgttcgtaa aaccggcgag ccccatgccc
     1141 atcataccga gctcaaagca ggcgctgaag ccatcgcgcg ccttttcggc gatcttccct
     1201 tccttctgga cccagcttga gcggcgtttc gcgagataca cggtttcgag attttctttc
     1261 gtgaacggct tcccggcttt caggagttcg atcacacctt ccgcaagctg aacacccgtc
     1321 gtccatgcct cgtcaacgcc ggaccccttc agcatgttcg tgctgccgga ac
//
LOCUS       DAOOBX010000267         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_104943_length_1369_cov_3.903349, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000267 DAOOBX010000000
VERSION     DAOOBX010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104943_length_1369_cov_3.903349"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1369)
                     /gene="rpoB"
                     /locus_tag="PLY30_04100"
     CDS             complement(<1..>1369)
                     /gene="rpoB"
                     /locus_tag="PLY30_04100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420648.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPW65323.1"
                     /translation="IKEFFGRSQLSQFMDQTNPLAEITHKRRLSALGPGGLSRERAGF
                     EVRDVHPSHYGRVCPIETPEGPNIGLIASLSTFARVNGIGLLESPYRKVTNGRVTEKI
                     DYLTADEEDKYVIAQANAPVDSSGKFQSDMISCRFRGDFPKKKPEEIDYMDVSPRQLV
                     SVAAALIPFLENDDANRALMGSNMQRQAVPLLTTEAPLVGTGMERYVAVDSGAVLAAK
                     SKGTVKAVSSEEIVIDDFVYKLKKFKRSNAGTCINQRPLVRVGDKVKRGDLIADGAGT
                     QGGDLALGRNVLVAFMPWRGYNFEDAIIVNERLCKDDVYTSLHIEEFEIEARDTKLGN
                     EEITRDIPNVSEESLKDLDKEGVVCIGAEVKPGDLLVGKITPKSEIELSPEEKLLRAI
                     FGEKAGDVRDASLTVPPGVEGVVVDVKVFARKESAAKTKEERRQENKDINEIKERYND
                     RIDLLK"
BASE COUNT          265 a          410 c          382 g          312 t
ORIGIN      
        1 gcttgagaag gtcgatgcgg tcgttgtatc gctccttgat ctcattgata tccttgttct
       61 cctgacggcg ttcttccttg gtctttgccg cggattcctt gcgggcgaac actttgacgt
      121 cgacaaccac cccttcgacg cccgggggca cggtgagaga agcgtcgcga acatctccgg
      181 ccttttcacc gaaaatcgcc cggagaagtt tttcttcagg agaaagctcg atctccgatt
      241 tcggcgtgat cttgccgaca agcagatcgc ccggcttcac ttccgcaccg atgcaaacaa
      301 caccttcctt gtcgaggtcc ttgagagact cttcgctgac gttcgggatg tctcgcgtga
      361 tctcttcgtt gccgagcttc gtatcgcggg cttcgatctc gaattcctcg atgtgcagcg
      421 acgtatagac gtcatccttg caaagacgct cgttgacgat aatcgcgtcc tcgaagttgt
      481 agccgcgcca cggcatgaag gctacgagca cgtttcgccc gagcgcgaga tcgccgccct
      541 gcgtgccggc accgtccgca atgaggtcgc cgcgcttcac cttgtcgccg actctgacga
      601 gaggcctctg gttgatgcag gtcccggcgt tggaacgctt gaacttcttg agcttgtaca
      661 cgaaatcatc gatcacgatc tcttccgacg acacggcctt gacggttccc tttgacttcg
      721 cggccaacac ggcaccggaa tcgaccgcca cataacgctc catcccggtg ccgactaacg
      781 gcgcttcggt ggtaagaagc gggacggcct gacgttgcat attcgaaccc ataagagcgc
      841 ggttcgcgtc atcgttttcg aggaacggaa tcagtgccgc cgccacagac acgagctgac
      901 ggggcgacac gtccatataa tcaatctctt ccggcttctt ttttggaaaa tcaccgcgaa
      961 aacggcagga gatcatgtcg ctctggaact ttccgctgga atccaccgga gcgttcgcct
     1021 gcgcgatcac gtatttatcc tcttcgtccg ccgtgaggta atcgatcttt tctgtgactc
     1081 gaccgttcgt caccttacgg taaggacttt cgagaaggcc gataccattg acgcgcgcga
     1141 acgtactgag cgacgcaatg agaccgatgt tcgggccttc cggagtttcg atcggacaaa
     1201 cgcgcccgta atgggacggg tggacgtcac gaacctcaaa gccggcgcgc tcgcgggaaa
     1261 gaccgcccgg gccgagggcc gagaggcggc gtttgtgcgt gatttctgcc agcgggtttg
     1321 tctggtccat aaactgcgac agctggctgc ggccgaagaa ctccttgat
//
LOCUS       DAOOBX010000268         1358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_106160_length_1358_cov_3.104375, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000268 DAOOBX010000000
VERSION     DAOOBX010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106160_length_1358_cov_3.104375"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1358)
                     /locus_tag="PLY30_04105"
     CDS             complement(<1..>1358)
                     /locus_tag="PLY30_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65324.1"
                     /translation="LPADLIETVGRAQILPSGKFAQNDGLYQVSRKTSDGKFERTGYA
                     YIVTEYGILRYSVVNTAAGEAKPQGSSYTVAGGKTMPLTPLDLMIRNDRSLGSQGRID
                     RLAFDLASRGEDFSMFLDDEFPTQFEFRSWRSLAGRRDIDRFVINGKNQSMVRPMTED
                     QGARILAGMAERAESIRSELRLQQDGAVTEVVRQDTVAGPAEATAEPDLGVAGLAAAL
                     PATREAFVSVIKSNLLAAAPAAGIELSEAAKRLQMMTPARDPRLQGKLGFYTDPAGAD
                     AFGIMALNSQKAEAFYQKIQGKTVLTLEERDELQDIMAELLVALAHENEHAHGGAEVR
                     AYRKSIDVAKLVKMPKFDLLYVLLDQARAEEAELKRVQDALQLVDQKGMTVRRAAIDI
                     LSSLNNSRTVAILSPELSAINGVESVGDVAGLGKYLRDARYIASANHTVFFIEDAKLK
                     MP"
BASE COUNT          265 a          463 c          353 g          277 t
ORIGIN      
        1 cggcatcttc agcttcgcgt cctcgataaa gaacacggtg tgattcgcgc tcgcgatata
       61 tctcgcgtcc ctgaggtatt ttccaagccc cgcgacatcc ccgaccgact ccacgccgtt
      121 gatcgccgag agttcggggc tcaggatcgc cacggtccgg gagttgttca aactgctcag
      181 aatatcgatc gcggcgcggc gcacggtcat tcctttctga tccacaagct ggagagcgtc
      241 ctgaactcgt ttcaattccg cttcctcagc ccttgcctga tccagaagga catacagaag
      301 atcgaatttc ggcatcttca cgagttttgc cacatcaatc gacttccggt aagcgcgaac
      361 ctcggcgccg ccgtgcgcgt gttcgttttc atgagcgaga gccacgagga gctccgccat
      421 aatatcctga agctcatcgc gctcttcgag ggtcagcacc gtcttcccct ggatcttctg
      481 atagaaagcc tccgcttttt ggctgttcag ggccataatg ccgaacgcat ccgcacccgc
      541 gggatccgta tagaaaccga gcttgccctg caatctcgga tcccgggccg gcgtcatcat
      601 ctgcaaacgc ttcgcagcct ccgaaagctc gattccggcc gccggggccg ccgccaggag
      661 attcgacttt atcacagaca caaaagcctc tctggtcgca ggcagggccg ccgcaagccc
      721 agcaacaccc agatccggtt ctgccgtcgc ctccgccggc ccggccacgg tgtcctgccg
      781 cacaacctcc gtcaccgccc catcctgctg caacctgagt tcagaacgga tgctctccgc
      841 cctttccgcc attcccgcaa ggatcctcgc accctggtct tcggtcatcg gccggaccat
      901 cgactgattc ttgccgttaa tcacgaacct gtcgatgtcc cgccggcccg caagcgaccg
      961 ccagcttctg aactcgaact gcgtcgggaa ctcgtcatca aggaacattg aaaaatcctc
     1021 tccccgggac gccaggtcga acgcgagacg atcgatccgg ccctgcgaac ccaggctgcg
     1081 atcattccga atcatgagat cgagcggggt cagcggcatc gttttgccgc cagcaacggt
     1141 ataagaggat ccctggggct ttgcttcacc cgcggcggta ttcaccaccg aataccggag
     1201 aatcccgtac tcggtcacga tatacgcgta tccggtccgc tcgaacttcc cgtccgacgt
     1261 cttccggctg acctgataga gaccgtcgtt ctgcgcgaac ttgccggacg gaaggatctg
     1321 cgcgcggccg acggtctcta tcaggtcagc cggaagac
//
LOCUS       DAOOBX010000269         1347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_107361_length_1347_cov_3.173375, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000269 DAOOBX010000000
VERSION     DAOOBX010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107361_length_1347_cov_3.173375"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PLY30_04110"
     CDS             <1..739
                     /locus_tag="PLY30_04110"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPW65325.1"
                     /translation="LALVNYLGSHNPVQGQLKIVLGALAIVAVPWILVAKQPDLGSAL
                     LFVPMVLTILFLWGIRWRYLIIALAGVLIAAPLAWEMLKEYQRKRILVFLNPNLDPLG
                     SGYTAVQSKIAVGSGGLFGKGYLAGTQTQLHFVPEHHTDFIFCVIGEELGFIGSMLVL
                     CLYAYLFHSIFLIMQHTTDIKAKLLSGGVLALLVAHVLINVGMTIGLMPIAGVPLPLV
                     SYGGSSFVMNCIALGLVLSVYKERSIF"
     gene            933..1006
                     /locus_tag="PLY30_04115"
     tRNA            933..1006
                     /locus_tag="PLY30_04115"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1115..>1347
                     /locus_tag="PLY30_04120"
     CDS             1115..>1347
                     /locus_tag="PLY30_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65326.1"
                     /translation="MFKSFIKKNKKLARRFLSGKFPFHGFERVARFEEDEPFEILGPH
                     AESHARRMTVRAFFPRAAEAWVRLPGEDRRQAM"
BASE COUNT          258 a          382 c          383 g          324 t
ORIGIN      
        1 gctcgcgctt gtcaattacc tcgggagcca caatcccgtc cagggccagc tgaagatcgt
       61 tctcggcgcg ctcgcgatcg tggcggtacc ctggatcctg gtcgcgaaac agcccgacct
      121 cggcagcgcg cttctctttg tgccgatggt cctgactatc ctcttcttgt gggggatccg
      181 ctggcggtat ctcatcatcg cgctcgcggg agtccttatc gccgcgccgc tcgcctggga
      241 gatgctcaag gagtaccagc ggaagcgcat cctcgtcttc ctgaatccca atctcgatcc
      301 gctcggatcc ggatacaccg cggtgcagtc caaaatcgct gtcggctccg gcggtctttt
      361 cgggaaagga tacctggcag gcacacagac gcagcttcat ttcgtgcccg agcaccacac
      421 ggactttatt ttttgcgtga tcggggagga gctcggtttc atcgggagca tgctggtcct
      481 ctgcctttac gcctacctct tccattcgat cttcctgatt atgcagcaca cgaccgatat
      541 caaggccaag ctcctttcgg gcggcgtgct cgccctgctc gtggcccatg tgctgattaa
      601 tgtcgggatg accatcggcc tcatgccgat tgcgggggtg ccgctcccgc ttgtcagcta
      661 cggcggttcc tcgtttgtca tgaactgcat cgcgctcggg ctcgttctga gcgtctacaa
      721 ggaacggtcg atcttttaat cgtttgcgaa taccagccgc tgtcctgttt acgaagggcg
      781 caacaacggc acaaagtgga aatggcgctg tagctgaaag cgtcacaagt cgttataatc
      841 ggaacatatc ggggtgccat gatttccatt gagaagtatt taaggcaatc atggcagtct
      901 ccgttgtctg ttgaaaagat tacaaggttc accggggtgt agctcaggtg gctagagcgc
      961 ttgctttggg agcaagaagt cggaggttca aatcctctca ccccgactct tttagaacag
     1021 cgatatggaa gtcgggacta cggcgggcga agagctgttt atcccttcgg ttcgtctttt
     1081 cgtaagcggt ttggtttcgg aagaaacatg acccatgttc aaatcattca tcaaaaaaaa
     1141 taaaaagctg gcccggcggt tcctttccgg gaaatttccg tttcatggat tcgagcgtgt
     1201 cgcccgcttc gaagaggacg agccgttcga gatcctcggg ccccatgcgg aatctcacgc
     1261 ccggcgcatg acggtccgcg cctttttccc gagggccgcg gaagcctggg tccgtcttcc
     1321 gggcgaagat cgacggcagg cgatgga
//
LOCUS       DAOOBX010000270         1343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_107824_length_1343_cov_3.133540, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000270 DAOOBX010000000
VERSION     DAOOBX010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107824_length_1343_cov_3.133540"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLY30_04125"
     CDS             <1..479
                     /locus_tag="PLY30_04125"
                     /inference="COORDINATES: protein motif:HMM:NF039287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPW65327.1"
                     /translation="LWHRTASMIEKYFEGKVPQAAPEVWQTKLMKDTLALWRGVTDDV
                     LRYDPREALAKIWSVVTEANQFVEEKKPWSLAKDPAKHGELADVLYALAECVAHIGIL
                     IGPFMPATSERILSRLKVATAGRVSDEAGFGEPMTVPGTPIEKGAPLFPRLDEKTA"
     gene            476..1243
                     /locus_tag="PLY30_04130"
     CDS             476..1243
                     /locus_tag="PLY30_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPW65328.1"
                     /translation="MILTDTHLHLHFPQYDADREDVIRRAFAGGVGRLINIGTGLEDS
                     RKAVAVAERYPEVYAAVGYHPHESKNATAGDLAGLEALVAHPRVVAIGEVGLDYFHDH
                     SPRNVQMELFHTFLGWCRKHQKPLILHCREAYGDMLRILRETLPVPCRGVLHCFSSDA
                     ATMEKFLEMGFHIAFGGAITYKNNDALQEACRACPADRLVLETDAPYLAPQSKRGRRN
                     EPLYMIETAEFIAKLRGDTVDRIAAATTENAKKLFGL"
BASE COUNT          272 a          378 c          410 g          283 t
ORIGIN      
        1 atctgtggca ccggaccgcg tcgatgatcg aaaagtattt tgaaggcaag gtcccgcagg
       61 cggcgccgga ggtctggcag acgaaactca tgaaggacac gctcgcgctc tggcgtggtg
      121 tcacggacga tgtcctgcgg tatgatcccc gggaggccct cgcaaagatc tggtcggttg
      181 ttaccgaggc gaaccagttc gtggaagaga aaaagccgtg gtcgcttgcg aaagatccgg
      241 cgaaacacgg ggagctcgcg gatgttcttt atgctcttgc cgagtgcgtc gcgcacatcg
      301 gtatcctgat cggtccgttc atgcccgcga cttcagaacg gattctcagc cggttgaagg
      361 tcgccaccgc aggtcgggtc agtgacgaag ccggatttgg ggaaccgatg accgtgcccg
      421 gaacgccgat cgaaaaaggc gctcctctct ttccacgact cgatgaaaag accgcatgat
      481 cctgactgac acgcatctcc atcttcattt tcctcaatat gatgccgacc gcgaggatgt
      541 gatccggagg gcgttcgcgg gcggcgtcgg ccgcctgatc aatatcggta ccggtctcga
      601 agactcgcgg aaggcggttg cggttgcgga gcgttatccc gaggtgtacg ccgcggtcgg
      661 ctaccacccg catgaaagca agaatgctac ggccggggac ctggccgggc ttgaagcgct
      721 ggtcgcgcat cccagggtgg tggcgatcgg ggaggtggga ctcgactact ttcacgacca
      781 ttcgccaagg aacgtccaga tggagctgtt ccatacgttc ctcggatggt gccgcaaaca
      841 tcagaagccg ctcattctgc actgccgtga ggcttatggc gatatgctga gaatccttcg
      901 cgagaccctt ccggtgccct gccgcggggt cctccactgt ttttcctccg acgccgccac
      961 aatggaaaaa ttccttgaga tgggattcca tatcgcgttc ggtggcgcga tcacttataa
     1021 gaataacgat gccctccagg aggcttgcag ggcctgtccc gcggatcgcc ttgtgcttga
     1081 gaccgatgcc ccttatcttg ctccccagtc aaagcgggga cggaggaacg agccgcttta
     1141 tatgatcgag accgccgagt tcatcgcgaa gcttcggggc gatactgtgg accggatcgc
     1201 agcggcgacc acggagaacg ccaaaaaact gttcggtcta tgataaaaga catcctgaag
     1261 ttctttaaac gcgagcggtt atatcttctg ttggtcctgt tgctcggcgc tttctatgcg
     1321 ttcatgacgc tggggcccaa gcc
//
LOCUS       DAOOBX010000271         1341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_108025_length_1341_cov_3.669518, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000271 DAOOBX010000000
VERSION     DAOOBX010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108025_length_1341_cov_3.669518"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="murI"
                     /locus_tag="PLY30_04135"
     CDS             complement(<1..681)
                     /gene="murI"
                     /locus_tag="PLY30_04135"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPW65329.1"
                     /translation="MTRPVPARPIGVFDSGLGGLTVVRQLRRHLANEEILYLGDLAHL
                     PYGTKSTREIRRRSEECASYLLKQGVKALVIACNSASSVAYRSLSKSLPIPVLDVITP
                     AVEEALASSRSARIGVIATYATIASKSYAKALAKRRPDADYFMQSCPLFVQLAEEGWL
                     DNEIALGTARRYLEPVKKKSVDTLILGCTHYPLLQKVIAKVMGRSVRLIDSAIPTARR
                     LGKLLEERG"
     gene            complement(713..>1341)
                     /locus_tag="PLY30_04140"
     CDS             complement(713..>1341)
                     /locus_tag="PLY30_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW65330.1"
                     /translation="PSNPTGRVEAIETLKEIERITQNLGVYVVSDEVYKDLIYERENY
                     HIQGNHVITVNSFSKTYCMCGVRVGYLWSADKSIVEAVCEAKTHTSMNTNLVGQDMAL
                     HAMRAPKEYILHQQEIWRARRDFMYQGLLDLGFDLWKPEGAFYVLPRCRNAREFLSLL
                     VREYKVIAYLGEWFGAPDRLRFSYALDVKKIEEGLGRIRDAMKKVSVL"
BASE COUNT          283 a          397 c          379 g          282 t
ORIGIN      
        1 cccgcgctcc tcaagcaatt ttccgagccg ccgggctgtc ggaatcgcgg aatcgatcag
       61 ccggaccgag cgccccatga cctttgcgat gaccttctga agaagcggat aatgggtgca
      121 tccgaggatc agggtatcga ccgatttttt tttcacgggt tcgagatatc gcctggccgt
      181 tccgagcgcg atctcgttat cgagccatcc ttcctcggca agctgcacga aaagcgggca
      241 ggactgcata aaataatccg catccggccg ccgtttcgca agggctttgg cgtagctttt
      301 gctcgcgatt gtcgcgtagg tcgcgatcac gccgatccgg gcactccggg aacttgccag
      361 cgcctcttcc accgcgggag tgatgacatc aagaacaggg atcggaaggg acttgctcaa
      421 ggaacggtaa gccacgctgg aagcggagtt gcaggcaatc acaagcgctt tgactccctg
      481 tttcagaaga taactggcgc attcttcgga gcgccggcga atctcgcggg tcgacttggt
      541 gccgtacgga agatgcgcca gatcgccgag gtagagaatc tcttcgttcg cgagatgtct
      601 ccggagttgg cgaacgaccg tcagccctcc gagcccggaa tcaaaaactc cgatagggcg
      661 cgcgggaacg ggacgcgtca taggcgaacc gggtccacaa ggcgccggag cattacagga
      721 cgctcacttt tttcatggcg tcgcggatcc gccccagacc ttcttcgatc tttttgacat
      781 caagggcgta gctgaaacgc aggcggtcgg gcgcgccgaa ccattccccc aagtaggcga
      841 tgaccttgta ttcccggacc aggaggctca ggaattcgcg cgcgttcctg cacctgggaa
      901 gcacataaaa agcgccttcc ggtttccaca gatcgaaccc caggtccaga agaccctggt
      961 acatgaaatc ccggcgcgcg cgccagatct cctgctgatg caggatatat tcctttggcg
     1021 ccctcatggc gtggagcgcc atgtcctggc ccacgagatt cgtattcatt gacgtgtggg
     1081 ttttcgcctc gcaaacagcc tccacgatgc ttttatccgc gctccacaga taaccgaccc
     1141 gcacgccaca catacaatag gtcttggaaa aggaattgac ggtgatgacg tgattaccct
     1201 ggatgtgata attctcgcgc tcatagatca ggtccttgta gacctcatcc gaaacgacat
     1261 agacgcccag attttgcgtg atccgctcga tctccttcag cgtttcgatg gcctctacgc
     1321 gtcccgtggg gttcgacggc g
//
LOCUS       DAOOBX010000272         1334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_108828_length_1334_cov_2.453479, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000272 DAOOBX010000000
VERSION     DAOOBX010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108828_length_1334_cov_2.453479"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..>1334)
                     /locus_tag="PLY30_04145"
     CDS             complement(74..>1334)
                     /locus_tag="PLY30_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPW65331.1"
                     /translation="VREGKTKVAIGHDHRWSAEFLYRGLSQGLASTGLTVVQIGLVPT
                     PVVYFYVATRDMDAGISLTGSHNPPDENGFKFQHATRSFYGADIQKLRGLIEKNDFVT
                     GTGKVETPDNAALLKAYEDYIADIFKFTKKLKVVIDTGHGMGATVAPDLIRRFGHDLT
                     VLYGNLDPKMPDHQADPSVPENMRDLQKKVVELKADIGIAFDGDADRIGIVNEKGEII
                     YGDKLLLIYARELLSRKPGAIIIGDVKSSKQVYDGIAKAGGKPLMWKTGHSLIKAKMK
                     EVHAEMAGEMSGHMFFKDRWLGFDDAIYAACRILEIADKVNAPFSSLLADVPNLYSTP
                     EIRFDCAEAIKFKVVAKAVENFKKLYNVVDIDGARVEFPDGWGLVRASNTQPVLVMRF
                     EAQTEKRLNEIRAIVENEVRKLTQEMS"
BASE COUNT          272 a          392 c          366 g          304 t
ORIGIN      
        1 cctttcattt ggaccacacg gggatcaggc gccgcttcgc ttcgattgac cggaacgggg
       61 accgtacagt tcatcagctc atctcttgag tgagcttcct gacttcgttc tcgacaatcg
      121 cacggatctc gttcaggcgt ttttcggtct gggcctcgaa acgcatcaca agcaccggct
      181 gggtattaga ggcgcgaaca agcccccatc cgtcagggaa ttccacccgg gcgccgtcaa
      241 tgtctaccac gttgtaaagt tttttgaagt tctcgacggc cttcgcgacc accttgaact
      301 tgatcgcttc ggcgcagtcg aaacggattt cgggcgtcga atagaggttc ggaacatccg
      361 ccagaagcga ggaaaaaggc gcgttcacct tgtcggcgat ctcgaggatg cggcaggcgg
      421 cgtagatcgc gtcatcgaaa ccaagccagc gatccttgaa gaacatatgg ccgctcatct
      481 cgccggccat ctccgcgtga acctctttca tcttggcctt tatcagggag tgccccgttt
      541 tccacatcag cggcttcccg ccggccttcg cgatgccgtc atagacctgc ttggagcttt
      601 tcacgtcccc gatgataatc gcccccggct tgcgactgag cagctcacgc gcgtagatca
      661 gcaggagttt atcgccatag atgatctccc ctttttcatt cacgatcccg atccggtccg
      721 catcgccgtc aaaggcgata ccgatatcgg ccttcagctc aacgactttc ttctggaggt
      781 cgcgcatgtt ttcgggaacc gaaggatcgg cctggtggtc cggcatcttc ggatcgaggt
      841 ttccgtaaag gacggtcagg tcatggccaa agcggcggat caggtccggg gcgacggtag
      901 cccccatccc atggcccgta tcgatgacaa ccttgagctt ctttgtgaat ttgaaaatgt
      961 ccgcgatata gtcctcatag gctttcaaaa gcgccgcgtt gtcgggtgtc tcgaccttgc
     1021 cggtccccgt cacgaaatca ttcttttcga tcaggccgcg caacttctgg atatccgcgc
     1081 cgtaaaagga gcgtgtggca tgctggaact tgaaaccgtt ctcatccgga gggttgtggg
     1141 atccggtcag ggaaatcccg gcatccatat cccgggtagc gacataaaaa taaacgacag
     1201 gggtcgggac aagtccgatc tggacgacgg taaggccggt actcgcgagg ccttgcgaaa
     1261 gcccccgata aaggaattcg gcgctccagc gatgatcatg accgatcgcg actttggtct
     1321 tgccttcccg gacc
//
LOCUS       DAOOBX010000273         1332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_109037_length_1332_cov_3.556774, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000273 DAOOBX010000000
VERSION     DAOOBX010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109037_length_1332_cov_3.556774"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..>1332)
                     /locus_tag="PLY30_04150"
     CDS             complement(181..>1332)
                     /locus_tag="PLY30_04150"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPW65332.1"
                     /translation="IPVGKQFGVVLVVEDEVTFEVATFRAEGGYEDGRHPTRIAFTVP
                     EEDARRRDFTVNGLFYDPFKKEILDFVGGQADLSQKVIRAIGDPEERFREDKLRLLRA
                     VRFASTLGFEIETKTWNAVRAHAADIHEVSPERIREELVKIFTRQGAERGFVLLRESG
                     LMKEILPEIEAMKGVEQPAQFHPEGDVFEHTRLLFAHLRPPVSEVLAFSALFHDVGKP
                     GTQAVRKGRLTFYEHSEEGAKLARDIMKRLKFPNATTDAVYECVLNHMKFMNVKKMRS
                     GKLKMFLLRPTFAEEMELHRADCLASHGMLDNYEFLQEKLKEYAHEELKPKPFINGND
                     LIAMGMPAGPAMKPVLEEAYELQLEKTLTDREASLAWARQKIASMKSQS"
BASE COUNT          254 a          430 c          342 g          306 t
ORIGIN      
        1 agggctcatt cattatctcc ggagaaacgt ttgtagcgca ccatttcgcc cttcgcgcag
       61 acctcgccgc tcgggaacgt ttcggccccg cgatccgcct tgatgaccat ctccgctctt
      121 tttcataccg tctcccttgg gctgatccgg ttcactgagc cattatacac ataatcttcg
      181 tcaactttga cttttcattg aagcgatctt ctgccgggcc cacgcaagcg acgcctcccg
      241 gtccgtcagc gttttctcca gctgaagttc gtaggcctct tccagaacgg gcttcatcgc
      301 gggtccggcc ggcattccca tggcgatcag gtcattcccg ttgatgaacg gcttcggttt
      361 caactcctca tgggcgtatt ccttcaactt ctcctgcagg aattcgtagt tgtcgagcat
      421 gccgtgactc gcgaggcagt ccgcacggtg cagttccatt tcctccgcga aggtcggccg
      481 caacaggaac atcttgagct tccccgacct catcttcttc acattcatga atttcatgtg
      541 gttgaggaca cattcgtaaa cagcatccgt cgtggcgttc gggaacttca gccgcttcat
      601 gatatcgcgc gcgagctttg ccccttcctc ggagtgttcg tagaaggtca accgcccttt
      661 ccggaccgcc tgcgttccgg gtttgccgac atcatgaaaa agggccgaga acgcaagcac
      721 ctcggacacg ggcggacgca gatgcgcgaa caaaaggcgc gtatgttcga agacatcccc
      781 ctcgggatgg aactgcgccg gctgctccac gcccttcatc gcttcgatct cgggaaggat
      841 ctccttcatc aaacccgatt cccggagcag cacgaagccg cgttcggcac cttgccgcgt
      901 gaaaatcttg acgagttctt cacgtattct ttcggggctc acctcatgga tatcggccgc
      961 gtgggcccgg accgcgttcc acgtctttgt ttcgatctca aaaccaaggg tcgaggcaaa
     1021 acgcaccgcg cgcaggagtc tgagcttgtc ttcccggaac cgctcctccg gatcgccgat
     1081 cgcgcgaatc actttttggg agagatcggc ctgcccaccc acaaaatcca ggatctcttt
     1141 tttgaaagga tcgtagaaaa ggccgttcac cgtaaaatca cggcgccgcg catcctcttc
     1201 cggaaccgtg aaggcgatcc gggtcggatg ccggccgtcc tcatagccgc cttccgcccg
     1261 gaacgtcgcg acctcgaagg tcacctcgtc ctcgaccacc aggacgacgc cgaactgttt
     1321 gccgacgggg at
//
LOCUS       DAOOBX010000274         1327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_109618_length_1327_cov_3.466981, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000274 DAOOBX010000000
VERSION     DAOOBX010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109618_length_1327_cov_3.466981"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /gene="tsaD"
                     /locus_tag="PLY30_04155"
     CDS             <1..892
                     /gene="tsaD"
                     /locus_tag="PLY30_04155"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPW65333.1"
                     /translation="LAEAKIRAPDLDLIAVTQGPGLIGSLFVGVSFAKALAYELGIPL
                     VGVNHMEAHLAANFFDRPAPEKFIGLLVSGGHTTITYCEKGKIRVLGETIDDAVGEAY
                     DKVAKILDLGYPGGPVIDRLAREGNPRAVQFTCPRLGQFSGKSSPSFEGGSREAHDTH
                     RFDFSFSGIKTAVLYLVKDPKTGQPKPDAPSSADIAASFQHAVIDWLVTKTLDAAGFK
                     GVSDIVVGGGVSANSHLRNKLTREARAKGVEVWFPPFPLSTDNGAMIARRGFGLFQQG
                     RRSSFRMTGEPSLAMAAGGR"
     gene            1087..>1327
                     /locus_tag="PLY30_04160"
     CDS             1087..>1327
                     /locus_tag="PLY30_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPW65334.1"
                     /translation="MTPQDVETIYRLLMAAGLGAAIGLERELSMKEAGFKTYTLVCIG
                     SALMMIVSINIFETYRGITVVDPARIAAQVVTGIGF"
BASE COUNT          246 a          377 c          424 g          280 t
ORIGIN      
        1 gctcgcggag gcgaaaatcc gggccccgga cctcgatctg atcgcggtga cgcaggggcc
       61 gggactgatc ggctcgcttt tcgtcggcgt ctccttcgcg aaggcgctcg cctatgagct
      121 cggcatcccg cttgtcggcg tcaatcatat ggaggcgcac ctcgccgcga attttttcga
      181 ccggcccgcg cctgaaaagt tcatcggact cctcgtctcc ggcgggcaca cgacgatcac
      241 ttattgcgaa aaagggaaga tacgcgtcct cggcgaaacg atcgacgacg cggtcggcga
      301 agcctacgac aaggtcgcga agatcctcga tctcggttat cccggcggtc ccgtgatcga
      361 caggctcgcc cgggagggga acccgcgcgc ggttcaattc acgtgcccca ggttggggca
      421 attttccggg aagtcttccc cttctttcga gggggggagc cgggaggctc atgataccca
      481 tcgctttgat tttagttttt cgggcatcaa aaccgcggtg ctttacctcg tgaaagaccc
      541 gaagacggga cagccgaagc cggacgcgcc gtcgagcgcg gatattgccg cgagtttcca
      601 gcacgcggtt attgattggc tggtcacaaa aacactcgac gcggccgggt tcaagggtgt
      661 atcggatatc gtggtcggcg gaggggtgag cgcgaactcg catctccgga ataaactgac
      721 ccgggaggcg agggccaagg gggtcgaggt ctggtttccg ccgttcccgc tttcgaccga
      781 taacggcgcc atgatcgccc ggcgcggatt cgggcttttt cagcaaggcc gccgatcgtc
      841 tttcaggatg acgggagagc cgtctttagc aatggccgcg ggtggccgtt agtaatggta
      901 ccggacggcc ggtctcacca aacaccagat gctgtttttc aatgaccggc gagccggagt
      961 tgcaaatacg ttaacgggta cggggggcga cgtgcggagc ggaaggtcgg ctatcatatc
     1021 tccgtacccg aaacttcgta ctccgtactg aatcgggagg ttcacatgtg tccatggctt
     1081 tcttttttga cccctcagga cgttgaaacg atttaccgcc ttctcatggc cgccgggctt
     1141 ggcgccgcga tcgggcttga gcgtgagctc agcatgaaag aggcggggtt caagacctac
     1201 acgctcgtct gtatcggctc cgcgctcatg atgatcgtct cgatcaatat ttttgagacg
     1261 tacaggggta tcacggtcgt ggatcccgcg cgcattgcgg cgcaggttgt gaccgggatc
     1321 gggttcc
//
LOCUS       DAOOBX010000275         1321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_110277_length_1321_cov_2.770142, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000275 DAOOBX010000000
VERSION     DAOOBX010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110277_length_1321_cov_2.770142"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /gene="lpxI"
                     /locus_tag="PLY30_04165"
     CDS             complement(<1..760)
                     /gene="lpxI"
                     /locus_tag="PLY30_04165"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HPW65335.1"
                     /translation="MKTMGIIAGNGRFPILVAEEARKAGYRVVTCGIEQEAEPALEKL
                     SDAFRWVKVGELGKLSRFLKDEQVHEAVMAGKIEKVRFFRENVRLDFDMMKVLMKLKD
                     YKDDSLLGGIARYLFDKGITLQDSTCLLKQYMPGPGPLGKLKPSRKALENIRFGFAVA
                     KQIAGADIGQTVVVKKKVVLAIEAIEGTDEAILRGSELGRGGIIVVKVEKPDQDARFD
                     VPAVGLTTLEGLMAAKAEAFAFEAYKTLFIGMDIF"
     gene            complement(854..>1321)
                     /locus_tag="PLY30_04170"
     CDS             complement(854..>1321)
                     /locus_tag="PLY30_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HPW65336.1"
                     /translation="ETVIGNDNLLMAYAHVAHDCVIHNGCTLANNGTLAGHVIVEDKA
                     IIGGLSAVHQFVRIGSLSITGGCSKVVQDIPPFMMVDGHPAKAFGLNSVGLDRAGFSA
                     KDKSDLKKAFKIIFKSGLILKNAVKEIRARVPGSAVIDTLVAFLEKSERGICG"
BASE COUNT          274 a          397 c          371 g          279 t
ORIGIN      
        1 caaagatgtc catcccgatg aaaagggtct tgtacgcctc gaaggcaaag gcttccgcct
       61 tcgcagccat gagaccctcg agggtcgtca gcccgaccgc ggggacgtca aaccgcgcat
      121 cctgatcggg tttctcgacc ttcacgacaa tgatgccgcc ccggccgagt tcggatcccc
      181 ggaggatcgc ctcatcggtt ccctcaatcg cctcgatcgc gagcacaacc ttttttttga
      241 cgaccacggt ctgcccgata tcggcccccg cgatctgttt ggccacggcg aacccgaacc
      301 ggatgttctc gagagctttc cgggacggct tcagttttcc gagagggccc ggaccgggca
      361 tatactgctt gaggaggcag gtcgagtcct ggagggtaat acccttgtcg aacaggtatc
      421 gcgcgattcc gccgagaagg gagtcgtcct tgtagtcctt gagcttcatg aggaccttca
      481 tcatgtcaaa gtcaagccgg acattctccc tgaagaaacg gactttctcg atcttgccgg
      541 ccataacggc ctcgtggacc tgctcatcct tcaggaaacg ggaaagcttt ccgagctccc
      601 caaccttgac ccagcgaaag gcatcggaga gtttctcgag agcgggttcg gcttcctgtt
      661 cgatcccgca ggtcacgacg cggtagccgg ccttgcgcgc ctcttcggcc accaggatgg
      721 gaaaacgccc gttgcccgcg ataatgccca tggttttcat gtttttaccc cgtctgatat
      781 cccggttgga ccggttacag aatcaatgat ctttaccgca aaagaggcgc agggcaaatc
      841 gcactccggc gagttaaccg cagattccgc gttcggattt ctcgaggaat gccacaagag
      901 tatcgatcac ggcgctcccg ggaacccgcg cgcggatctc tttgacggcg ttcttaagga
      961 taaggcctga cttgaagatg atcttgaagg ctttcttgag gtcggatttg tctttcgccg
     1021 aaaatcccgc ccggtcgaga cccacggaat tgaggccaaa cgccttggcc ggatgcccgt
     1081 ccaccatcat gaaaggcggg atgtcctgga ccaccttgga gcaaccgccg gtaatcgaaa
     1141 gcgacccgat gcggacaaac tgatgcacgg cggaaaggcc gccgatgatc gccttgtctt
     1201 ccacaatgac atgcccggca agggtgccgt tattggcgag cgtgcaaccg ttatgaatga
     1261 cgcagtcgtg cgcgacatgc gcgtaggcca tcaggagatt gtcgttcccg atcacggtct
     1321 c
//
LOCUS       DAOOBX010000276         1311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_111550_length_1311_cov_3.457006, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000276 DAOOBX010000000
VERSION     DAOOBX010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111550_length_1311_cov_3.457006"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1311
                     /locus_tag="PLY30_04175"
     CDS             <1..>1311
                     /locus_tag="PLY30_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPW65337.1"
                     /translation="KSGFRKLSSIVYNRIARSILPIGPIADTQSGLKAFSRETALATV
                     DEVKDVTMSFDTEFLLLAREKGFRVEEVPIFWKDSQVGTSISVFMQMFRMIEGLLGQR
                     LRMLARSRDRAGTSAGITISRTLTRFAIVTVRTLQGFLRISRDFRKTDRHRPAAGATA
                     LREADLDATLVSDLSAVARSIVGDAVRVLDQFRRRKTGLETKMKTETGTENRTVVTEA
                     DEAVQGFIVPEIQRLFPEHFVIAEEKMSGEIARRNRENEGSDFVWVLDPIDGTREFLK
                     PDSDFYAISLALFHKGRPVLAVVHSPHAGMIEASSARDGVYVNGKPVTPVTESPAETF
                     RAVIDDAPQTELVPVGTLGGMPWSDVSHSTRSVALNLARIAFAGQDGEAPDLFWKGGS
                     RGPALWDIAGPSYLLKKAGGLALSLDQGTVTDAQDAVSRAILDPA"
BASE COUNT          261 a          371 c          404 g          275 t
ORIGIN      
        1 caaaagcggt ttccgcaagc tttcgagcat cgtctataac cgtatcgcac gatcgatact
       61 cccgatcggt ccgatcgccg atacccagtc cggtctcaag gcgttcagcc gtgagaccgc
      121 ccttgcaacc gtggacgaag tgaaggatgt caccatgtcc tttgataccg agttcctcct
      181 cctcgcccgg gaaaaaggtt tccgggtaga agaagtaccg atcttctgga aggacagcca
      241 ggtcggaacc tccatttctg tttttatgca gatgttccgg atgatcgaag gtctcctggg
      301 gcagcgtctg cggatgctcg cgcggtcgcg ggaccgtgcc ggtacctctg ccggaataac
      361 gatctcccga accttgaccc gttttgcgat cgtgacggtt cggacgcttc aagggttcct
      421 caggatctcc cgggatttca ggaaaacgga tcggcaccgt cctgcggcgg gggcgaccgc
      481 gcttcgggaa gcggacctgg acgcgacgct agtgtcggat ctttcggccg tggcccgctc
      541 gatcgtgggc gatgcggtgc gtgttctcga ccaattccgt agacggaaaa cgggtcttga
      601 gacgaagatg aaaacagaga ccggaacgga aaaccggacg gtcgtgaccg aggcggatga
      661 agcggtccag gggtttattg tcccggaaat ccagaggttg ttccccgaac atttcgtgat
      721 cgcggaagaa aagatgagcg gtgagatcgc tcgcaggaac cgcgagaacg aaggatcgga
      781 ttttgtatgg gtcctcgacc cgatcgacgg gaccagggaa ttcctcaagc cggacagtga
      841 cttttacgcc atttcgctag cgctcttcca taaaggacgt ccggttcttg ccgtggtcca
      901 ctcaccgcat gccggtatga tcgaagcgtc ctcggcgcgt gatggggttt atgtgaacgg
      961 aaagccggtg actccggtca cggaatcccc ggctgaaacc tttcgggccg ttattgatga
     1021 tgcccctcaa acggaactgg ttccggttgg aaccctcgga gggatgccgt ggagtgacgt
     1081 ttctcacagc acccgttccg ttgccctgaa cctcgcgagg atcgcgtttg ccggtcagga
     1141 cggtgaggca cccgaccttt tctggaaagg cggttctcgc ggtcccgcgc tctgggatat
     1201 tgccggccca agctaccttc tcaaaaaagc gggcggactg gcgctgagtc tcgatcaagg
     1261 taccgtgacg gatgcgcagg acgccgtgtc ccgcgcgatc ctggatcccg c
//
LOCUS       DAOOBX010000277         1304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_112376_length_1304_cov_3.232986, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000277 DAOOBX010000000
VERSION     DAOOBX010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112376_length_1304_cov_3.232986"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PLY30_04180"
     CDS             complement(<1..983)
                     /locus_tag="PLY30_04180"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPW65338.1"
                     /translation="MNFLIKTFGCKVNQYESQAIRESLEKAGARELCGKSRSADLVIV
                     NTCTVTRSADRTTLYWIRRLKREHPGCRLAVTGCFAERDSELLETMPEVDAVFTNQQK
                     DSIAATLVAGKRSFGCAGASARKEYLPLSITRSEGKTRAHVKIQDGCNHSCSYCKVVL
                     VRGPSRSRPLPEILNEVRRLADSGYKEIVLTGIQLGAYGDFPSKRPEALVRVIEGCSG
                     IPGVERIRLSSIEPTDIHEPLIRAFRDFPKLCPQLHIPLQSGDDAILKGMNRRYTAAF
                     YTDLIARLREAIPDFCLTLDVMTGFPGEGERQFENTLETLARVRPLKSHVFP"
     gene            complement(1024..>1304)
                     /locus_tag="PLY30_04185"
     CDS             complement(1024..>1304)
                     /locus_tag="PLY30_04185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPW65339.1"
                     /translation="LADDDALLILHPEPGATAFPEWLESFRGIRDRVRSLNILIGPEG
                     GFTDDEIEWVRWRGKKKNVWVVSLGETVLRADTAFISVLGTLRFLGIV"
BASE COUNT          270 a          405 c          376 g          253 t
ORIGIN      
        1 gggaatacat gacttttgag cggacgcacc ctcgcgagcg tttccaaggt gttctcgaac
       61 tgccgttcac cttcaccggg gaatcccgtc atcacatcga gggtcaggca aaaatccggg
      121 atcgcctccc gcagccgcgc gatcaggtcg gtgtaaaacg ccgccgtgta acggcgattc
      181 atacccttca ggatggcgtc atccccgctc tgaagcggaa tatgaagctg gggacaaagt
      241 ttcggaaaat cccggaacgc ccggatgagc ggctcatgga tgtccgtcgg ctcgatcgaa
      301 ctgagacgga tccgctccac gcccggtatc ccggaacatc cttcgataac ccgaacaagg
      361 gcctccggcc gtttcgaggg gaagtcgccg tatgcgccaa gttgtattcc cgtcagcacg
      421 atctccttat agcccgagtc cgccaaccgc cggacttcgt tcagaatctc cggtaacgga
      481 cggctccggg acgggccccg caccagaaca accttacagt aactgcagga gtgattacac
      541 ccgtcctgga tcttcacgtg ggcacgcgtc tttccttcgc tccgcgtgat cgaaagcggc
      601 aggtattcct tgcgcgcgga cgctcctgcg caaccgaacg aacgcttccc tgccacaagc
      661 gtggcagcga tcgaatcttt ttgctggttc gtgaaaacgg catccacctc aggcatggtc
      721 tcaaggagtt cggagtcgcg ctccgcgaag caacccgtca cggcaagccg gcagccggga
      781 tgctcgcgct tcagccgccg gatccagtaa agcgtggtgc ggtcggcgga gcgagtcaca
      841 gtgcacgtat tgacgatcac aaggtccgcg gagcggcttt tcccgcaaag ttcacgcgcg
      901 cccgcctttt ccagggattc gcggatcgcc tgcgattcgt attgattgac cttgcagccg
      961 aatgttttga tcagaaaatt catgggctga gaaaaggtgt cggatgaccg gatactgggg
     1021 acttcagacg atcccgagaa agcgcagggt cccgaggaca ctgatgaagg ccgtgtccgc
     1081 cctcaggaca gtctcaccga ggctcacgac ccagacgttc ttttttttgc cccgccaccg
     1141 gacccactcg atttcatcgt ccgtgaaacc gccttcgggt ccgattaaga tattgagcga
     1201 ccgcacgcgg tcccggatac cgcggaacga ttcgagccat tccggaaacg ccgtcgcccc
     1261 gggttcggga tgaaggatca ggagcgcgtc atcgtccgcg agcg
//
LOCUS       DAOOBX010000278         1301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_112729_length_1301_cov_3.172552, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000278 DAOOBX010000000
VERSION     DAOOBX010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112729_length_1301_cov_3.172552"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="PLY30_04190"
     CDS             complement(<1..1162)
                     /locus_tag="PLY30_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65340.1"
                     /translation="MAAFEGTLRIGIGKDTAGRFVNFEVEPVTNENGVPSSLPTEAFF
                     RIIFPGDDTEEYRIAIQDFEVVNDEAETAVAPEENPADKIKREAEEVSRQAFTYVVAR
                     ERDDTLGITHGDLFEVADQYLSKGKHFTFQIFTIGDRTWFKVVDYSLNGTNISADIDP
                     DTVVYPQRLEVLTTFDERLDEVSSKSLQVREAWTDKVEGGQAFERFKLMLDGAAQLYL
                     ESAEFPELREKRKKILATYFFPDSIGHSPLRAEYALNWLHRFKDDDRASVVTPVGELA
                     CKYAVRFRRFVELLPAEDEDGVPESLRKEAKKVRAELMGRTQPFFNPSNTMKLTTQAL
                     RDIDEMNKDPHPRPRVTFKRRSEMRSEGARPELTMDLQFTNDFYDRISGLDEA"
BASE COUNT          252 a          403 c          349 g          297 t
ORIGIN      
        1 cggcttcatc cagaccgctg atcctgtcat aaaaatcatt ggtgaactgc aaatccattg
       61 tcagctccgg cctcgcgcct tcgctccgca tctccgagcg gcgcttgaac gtgacccggg
      121 gacggggatg aggatctttg ttcatctcgt cgatatccct cagggcctgc gtcgtaagct
      181 tcatcgtatt gctcgggttg aagaacggct gggtcctccc catcaactcg gcccgcacct
      241 ttttggcttc cttgcgaagc gattcgggaa caccgtcctc atcctcggcg ggaagcaact
      301 ccacaaaccg gcggaacctg acggcatatt tacaggccag ttcccccaca ggcgtgacca
      361 cggaggcccg gtcatcgtcc ttgaaccggt gaagccaatt gagggcgtac tcggcgcgaa
      421 gcggcgagtg accgatcgag tccgggaaaa aataggtcgc gagaatcttc tttcgtttct
      481 cccggagttc ggggaattca gcgctttcca gatacaactg cgccgcgccg tccagcatga
      541 gtttgaatct ttcaaaggct tgtcccccct cgaccttgtc cgtccaggct tcgcgcacct
      601 ggaggctttt cgaactgact tcgtccaggc gttcatcgaa cgtggtcaga acttcaagac
      661 gctgcgggta cacgaccgta tcggggtcga tgtccgcgga gatgttcgtg ccgttcagcg
      721 aatagtccac gaccttgaac catgtccggt cgccgatcgt gaaaatctga aaagtgaaat
      781 gcttcccttt gctgagatat tggtcggcca cctcgaacag atccccatgt gtgatcccca
      841 gggtatcatc ccgctcccgg gcgacgacat aagtgaaggc ctgccgggag acctcctccg
      901 cttcccgttt gatcttgtct gccggattct cctccggagc gactgcggtt tctgcctcat
      961 cgttcaccac ttcaaaatcc tggatcgcga tccggtactc ttcagtgtca tcgcccggga
     1021 aaatgatccg gaagaaggct tccgtgggca aggaggacgg cacgccgttt tcattcgtca
     1081 cgggctccac ttcaaaattc acaaaacgtc ccgcggtgtc cttcccgatc ccgatccgaa
     1141 gcgtcccttc aaaagcggcc atgtattccc ctgttgacat aaaatgcccg cgcagcggct
     1201 caagttcccg cgtcgctgcc gcgctgtctg ttttttcagg gaccggactt tcaacgcttc
     1261 tcgtttcgga ccgccgggac cggaactcgg ggcggggagg a
//
LOCUS       DAOOBX010000279         1298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_113143_length_1298_cov_2.231698, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000279 DAOOBX010000000
VERSION     DAOOBX010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113143_length_1298_cov_2.231698"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /gene="rplC"
                     /locus_tag="PLY30_04195"
     CDS             complement(<1..562)
                     /gene="rplC"
                     /locus_tag="PLY30_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPW65341.1"
                     /translation="MTRIFDEEGRQVPVTVLEVGPCFVTGLRTKEKNGYQAVQLGFDV
                     VKERRLSKPKLGLTKKAALPPLNIQREFRTDSLENLAVGSEVRVDNFEAGEFVDVIGT
                     SIGKGFQGVVKRLGYKGGASQGHGSMFGRAPGGIGSQAGGAGARKKVRKGKGLPGHLG
                     NERVHVENVKIMKVDGENNLLVVKGSI"
     gene            complement(603..914)
                     /gene="rpsJ"
                     /locus_tag="PLY30_04200"
     CDS             complement(603..914)
                     /gene="rpsJ"
                     /locus_tag="PLY30_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015493774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPW65342.1"
                     /translation="MAVKEKIRIKLKAYDHRVLDQSVQEIVATAKKTGAKVIGPIPLP
                     THIWKTTVLRGPTIDKKARDQFEVRTHKRLIELEEPTSRTVDALMKLDLPAGVDVEIK
                     L"
     gene            complement(921..>1298)
                     /gene="fusA"
                     /locus_tag="PLY30_04205"
     CDS             complement(921..>1298)
                     /gene="fusA"
                     /locus_tag="PLY30_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994301.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPW65343.1"
                     /translation="TVFDGSYHEVDSSEIAFKMAGIFAFKDAFKNAKPILLEPIMLVE
                     VLMPEEYMGDVIGDLNSRRCVIQELSNRGHLKSVRGLVPLAEMFGYATAVRSLSQGRA
                     TYTMEPNTYQEVPRQIAEKIIAG"
BASE COUNT          268 a          403 c          325 g          302 t
ORIGIN      
        1 ggatactgcc cttcacgacg agaagattgt tctcaccgtc gactttcata attttgacat
       61 tctcgacatg gacacgctca ttacccaaat gtccgggaag tcctttcccc ttgcgaacct
      121 tcttgcgggc cccggcaccg ccggcctggg aaccgatgcc acccggggcg cggccgaaca
      181 tcgatccatg tccctgggac gcgccgccct tatagccaag acgcttcacc acaccctgaa
      241 aacccttacc gatcgaggtg ccgatcacgt cgacaaactc accggcttcg aaattgtcca
      301 cgcggacctc gcttccgacc gcaagatttt caagactgtc tgtgcggaat tccctctgga
      361 tattgagggg tggaagcgcc gctttcttcg tcaggccgag cttcggcttc gagagcctgc
      421 gctctttcac aacatcgaac ccgagctgca ccgcctgata accgtttttt tccttcgtcc
      481 gaagccccgt cacgaaacac ggccccactt ccagaaccgt cacgggaacc tggcgtccct
      541 cttcgtcaaa gatcctcgtc attccgactt tttttcccaa aaggcctaac ataagattcc
      601 tcttagagct tgatctcgac atccacgccc gcgggcaggt caagtttcat gagagcatcc
      661 acggtgcgag acgtgggctc ttccagttca ataagacgtt tatgcgtgcg gacctcgaac
      721 tggtcgcgcg cttttttgtc gatcgtcgga ccgcggagca cggtcgtctt ccagatatgc
      781 gtcgggagcg gtatcgggcc gataaccttg gcgcctgttt ttttcgcggt cgccacgatc
      841 tcctgcaccg actgatccag cacccggtgg tcatacgctt tcagtttgat acggatcttt
      901 tccttcactg ccatgattga ttaccccgcg atgatctttt ccgcaatctg acgcggcact
      961 tcttgataag tattgggctc catcgtatag gtcgcgcggc cctgggagag cgaacggaca
     1021 gccgtcgcat atccgaacat ttcggcaagc ggaacgagcc cgcggacaga cttgaggtgt
     1081 ccgcgattac taagctcctg aatgacacag cggcgggaat tcagatcgcc gatcacatcc
     1141 cccatgtatt cttcgggcat gagcacctcg accagcatga tcggctcgag aaggatcggc
     1201 ttcgcgttct tgaacgcgtc cttgaacgca aaaataccgg ccattttaaa agcgatctcg
     1261 gaagagtcga cctcgtggta gctgccgtcg aaaacggt
//
LOCUS       DAOOBX010000280         1281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_115258_length_1281_cov_2.228385, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000280 DAOOBX010000000
VERSION     DAOOBX010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115258_length_1281_cov_2.228385"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLY30_04210"
     CDS             complement(<1..230)
                     /locus_tag="PLY30_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPW65344.1"
                     /translation="MQTFDAIEKRHSTREYKNKAVPKEMLEALVDAGRRAPSARAIEP
                     WEFIVVTERKTLDALAVFINTGAFLAQAPACIV"
     gene            complement(233..>1281)
                     /locus_tag="PLY30_04215"
     CDS             complement(233..>1281)
                     /locus_tag="PLY30_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPW65345.1"
                     /translation="HVASARALEGLLQRIRTVIPPEKIDTIIANHIEPDHSGAYPGLL
                     KFCPKAKMYGSARAGQGLERYYHRNFDFTPVKTGDELSLGKRTLVFIEIPMIHWPDSM
                     MTYLIGDQILFSNDAFGQHYATSERFDDEVPRETLMEETVKYYANILWPFSHLIAQRL
                     AEIEKLGIPIRMIATSHGLIWRKEPGKIISAYKKWAGNATEPKVVIVYETMWGSTARM
                     ARKIAEGVADAGVKHEVYDIAATDRTEVIYGLFNAKGYIFGSSNHDSGMLPNMVSFME
                     FLKGLHPRNRIGAAFGSYGWSGLAIKKLEETMKEAGVEIAQPALSVVYAPDASQLAAC
                     YEFGKKIAETVKKG"
BASE COUNT          239 a          390 c          359 g          293 t
ORIGIN      
        1 acaatacagg ccggcgcctg cgccaggaac gcgccggtat tgatgaacac ggcaagcgca
       61 tcgagcgttt tccgttccgt caccacgata aattcccacg gctcgatcgc gcgggcgctc
      121 ggcgcccgtc gtcccgcatc gaccagcgcc tcgagcatct ccttcggcac ggccttgttt
      181 ttgtattcgc gggtgctgtg gcgtttttca atggcgtcga atgtctgcat ggtcaacctt
      241 tcttcacggt ttccgcaatc tttttcccga attcgtaaca ggcggccagt tgtgaggcat
      301 ccggcgcgta caccaccgaa agggccggct gcgcgatctc caccccggct tctttcatcg
      361 tttcctcgag ctttttgatc gcgaggccgg accagccata ggaaccaaac gcggcaccga
      421 tccggttgcg gggatgcagt cctttcagga attccataaa ggacaccatg ttcggcagca
      481 tgccgctatc gtgattggac gacccgaaga tgtaaccttt ggcgttgaag agaccgtaga
      541 tcacttccgt gcggtccgtc gcggcaatgt catagacctc atgtttcaca cccgcgtccg
      601 caacgccttc cgcgatcttg cgggccatcc tggcggtcga accccacatg gtctcataga
      661 cgatcacgac tttgggttcc gtcgcgttac ccgcccattt tttataagct gagatgatct
      721 tgcccggctc cttgcgccag atgaggccat gggacgtggc gatcatccgg atcggaatcc
      781 ccagcttctc gatctccgcg agccgctgcg cgatcagatg gctgaacggc caaaggatat
      841 tcgcgtaata tttgacggtc tcttccatga gggtttcccg gggaacctcg tcatcaaaac
      901 gctcgctcgt ggcgtaatgc tgtccgaaag cgtcgttcga gaagagaatc tggtccccga
      961 tcaggtaggt catcatgctg tccggccaat ggatcatcgg gatctcgatg aaaaccagcg
     1021 tccgcttgcc gaggcttaac tcgtcgccgg tcttgacggg ggtgaaatca aaattccggt
     1081 gatagtaccg ttcgagccct tgccccgccc gcgcgcttcc gtacattttt gccttggggc
     1141 agaacttcaa aagtccggga taggcgccgg agtggtccgg ctcgatgtga ttggcgatga
     1201 tcgtgtcgat cttttccggc ggaatcacgg tccggatccg ctggagcaga ccttccaggg
     1261 cccgcgccga cgccacgtgc a
//
LOCUS       DAOOBX010000281         1279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_115435_length_1279_cov_6.515523, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000281 DAOOBX010000000
VERSION     DAOOBX010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115435_length_1279_cov_6.515523"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..952)
                     /locus_tag="PLY30_04220"
     CDS             complement(176..952)
                     /locus_tag="PLY30_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65346.1"
                     /translation="MRKRAAVLLLLLFLAAVYVPSAQAAMPTFDAINAALQELQNSIL
                     NSSFAQDLMLAAERLNQLKTQTLELFRFHAGFDEILNFVKGDPVRDFIDSMRTKIRDV
                     FSDAIFTTPQIEILQTGGSAEDLRTSLEAISGAIPEGNQRAYIPFEEMQVVEAFQLAN
                     QIREGGKDLRDAARQIQAQAETASPKGAVRLQAQGAAELMVLGQQTQESIAKLLELEA
                     TQIEQVSRREKDAERERLRFIKDTGEYLDGILDASGGEWR"
     gene            complement(945..>1279)
                     /locus_tag="PLY30_04225"
     CDS             complement(945..>1279)
                     /locus_tag="PLY30_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65347.1"
                     /translation="VKYILRLKKGHEKLASFGLNENEILAARELEVRPGHYSEVFIKF
                     DDHGVIAKVEPNPLEYWIATTDPADLARETDLRAASPLKTNLEIFETLARLFPGGASK
                     PRQEASHA"
BASE COUNT          266 a          379 c          359 g          275 t
ORIGIN      
        1 ataaaaaggc ctttgtaatc cgagcggtct ttcatcacga gaaagttctc gtgcaacacc
       61 gccacgacga acatgagcgg aaagatcact gccgcagccg aggctgtaac tccataggcg
      121 gatgcgaaaa gatccgggat ctgaagaacg ttcgtgaaaa gaaatccctg gttcgtcatc
      181 gccactcccc gcctgacgcg tccaggatgc cgtccagata ctcgcccgtg tccttgataa
      241 agcggagacg ctctctctca gcgtcctttt cgcggcgcga tacctgctcg atctgagtag
      301 cctcaagttc gagaagtttc gcgatcgatt cctgggtttg ctggccgagg accatcagtt
      361 ccgcagctcc ctgcgcctga agcctgaccg cgcctttggg gctcgctgtc tccgcttggg
      421 cctgaatctg tcgggccgcg tcccggaggt cttttccccc ttcgcggatc tgatttgcca
      481 gctgaaacgc ctcgaccacc tgcatctcct cgaacgggat atacgccctt tggttcccct
      541 cagggattgc gcccgatata gcctcaagcg aggtccggag gtcttcggcg cttccgcccg
      601 tctgaagaat ctcgatctga ggcgtcgtga agatcgcgtc cgagaaaacg tcccggatct
      661 ttgtcctcat ggaatcaatg aagtcacgga ccggatcccc tttcacgaaa ttcaggatct
      721 catcgaagcc cgcatgaaac cggaatagct caagggtctg ggttttcagc tggttcaagc
      781 gttcggcggc aagcatgagg tcctgcgcaa agctgctgtt caggatggag ttctgaagct
      841 cctgcagggc cgcgttaatg gcgtcaaagg tcggcatcgc ggcctgggcg gaagggacat
      901 aaacggccgc aagaaacagc aaaagaagga gaaccgccgc ccgcttacgc atgggaagcc
      961 tcctgccgcg gttttgacgc gccgcccggg aacaaacggg ccagcgtttc aaagatctcc
     1021 aggttcgttt taagaggcga cgcggcccga agatccgttt cccgggccag atccgcggga
     1081 tccgtcgtgg cgatccagta ctcgagaggg ttcggttcca ctttcgcgat caccccatga
     1141 tcgtcgaact tgataaagac ctccgaataa tgcccaggcc gtacctcgag ctccctcgcg
     1201 gcaaggatct cattctcgtt aagtccgaag gaggcgagct tttcgtgtcc tttcttcagg
     1261 cgcaggatgt atttgacgg
//
LOCUS       DAOOBX010000282         1274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_116108_length_1274_cov_3.528302, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000282 DAOOBX010000000
VERSION     DAOOBX010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116108_length_1274_cov_3.528302"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLY30_04230"
     CDS             complement(<1..324)
                     /locus_tag="PLY30_04230"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPW65348.1"
                     /translation="MSQLTQSIVKHQHASWVELRSDRLLANLEALRQRSGTSDALTVI
                     KANAYGHGLNEIAKILDGRVNFFGVATLREGLELKERHPGTPVFLFGHPFPHEVSAAI
                     GANLVL"
     gene            complement(343..1089)
                     /gene="tsaB"
                     /locus_tag="PLY30_04235"
     CDS             complement(343..1089)
                     /gene="tsaB"
                     /locus_tag="PLY30_04235"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPW65349.1"
                     /translation="MNKIPLMTNLLAVETSGPFLSVAVKKGGRPVRRAHMKGFSRHVE
                     SLIPLIDRLLKKEKLSIRDIGAFLIGRGPGSFTGLRVGFATLKGFLAVRERPCFGALS
                     LDLIAANVNPGRGDPGSNRVRTPILAVCLNAKRDKLYVRSYRYHKKGWKPLGAPAAEE
                     PGAWLGGLPEGTWIAGDGLAICRLGLLGKLGPLPEKHWYPDAATLIALYERKDPLLRK
                     LTRPKDLLPFYLRASEAEERLKTAGSRSRR"
BASE COUNT          270 a          376 c          370 g          258 t
ORIGIN      
        1 caggacaaga ttggcgccga tcgcggcgga aacctcatga ggaaaaggat gtccgaagag
       61 gaagaccgga gtcccgggat gacgttcctt cagttccagt ccttcgcgga gcgttgcgac
      121 gccgaagaaa ttgaccctgc cgtcaagaat cttcgcgatc tcgttcaacc cgtgcccata
      181 cgcattggcc ttgatcaccg taagcgcgtc ggaggtcccc gatctttgcc ggagggcttc
      241 aagattcgcg aggagacggt ctgaacgcag ttcgacccac gatgcgtgct gatgtttaac
      301 aatgctctga gtgagttgtg acatatgatt tccgccagga gctcagcgcc gagagcggct
      361 cccggctgtt ttaagccgtt cctcggcttc ggaagcccgc aaatagaacg gcaacaagtc
      421 tttcggtctg gtcagctttc ggagcaacgg atcctttctt tcatacagtg cgatcaatgt
      481 cgcggcgtcc gggtaccagt gtttttcggg aagcggcccg agctttccca aaagcccaag
      541 gcggcagatc gccagtccat cgccggcgat ccaggtacct tcgggcaatc ctccaagcca
      601 cgcgcccggc tcctcggccg ccggcgcccc gagaggtttc caccctttct tgtggtaacg
      661 gtacgaacga acataaagtt tatcacgctt ggcattcaaa cacacggcca gaatcggggt
      721 gcgaacccga tttgagccgg gatcaccccg ccccggattg acgttcgcgg cgatcaggtc
      781 gagcgacagg gcgccgaaac acggccgttc tcgtaccgca agaaagcctt tcagcgtcgc
      841 gaagccgacc cggagccccg tgaacgaacc cggaccccgt ccgatcaaaa aggcgccgat
      901 gtcccggatg gacaattttt cttttttaag gagccggtcg atcaagggga tcaagctctc
      961 aacatggcgt gagaagcctt tcatgtgagc gcggcggacg ggacggccgc cttttttaac
     1021 ggcgacgctc aggaaaggtc cggaggtttc gacggcgagg aggttcgtca tgagcggtat
     1081 tttattcaca aatcccggaa tgggaaaggc aaaagggacg cgcgtgcgcg gagaatttcc
     1141 ccggtgttgt ataatgcttc ccatgcaaaa gaacaccggc cccgcgctca gttcgcaatt
     1201 catctatgtc gaccgcgcgg aagccctttc ccgtctgacc gcggcccttc gtacggttga
     1261 ccgtgtcgcc ttcg
//
LOCUS       DAOOBX010000283         1274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_116117_length_1274_cov_2.979491, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000283 DAOOBX010000000
VERSION     DAOOBX010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116117_length_1274_cov_2.979491"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1274
                     /locus_tag="PLY30_04240"
     CDS             <1..>1274
                     /locus_tag="PLY30_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65350.1"
                     /translation="RAARKQVENQGLAEIVVIFKQLARLNRQLSSTEDGIRRIEEQAL
                     PAVGGPEFLEHDQDSKRSMESRLKDLKLNKSILVNTRTIYEQRLKHMLGRSPAVPSGI
                     NVETITFDTVERLTEQFGLGQRLIAGALLQDSANLKLAIKQAASMTGTQLTIAMPQVS
                     VDIARTVGYYALGDKFTVELIRADFNFSLDRLRGEKMRDEQVDAEIFQNVGKIKDLQD
                     EQAVLRRMLEQEKALLKELREIRENRAKLLAEIESQPELVRYRDRQWVTAVTELENAR
                     DKETGAELGVSLLEARLMDPDGFQEAVRVSLVNMKEALPEEKHAVLARALENFGLNED
                     GSRVALVSDEVLTARARELAKLVLPKGSTDFKPAPLERVMDAILLSMRYRLQTFVEKA
                     EADELDAEAMNVFSLYATAALGYGFGQESYGDV"
BASE COUNT          296 a          339 c          396 g          243 t
ORIGIN      
        1 cgcgccgcgc ggaagcaggt cgaaaaccag ggactcgccg agatcgttgt cattttcaag
       61 cagctcgcga ggctgaaccg gcaactctct agcaccgaag acgggattcg caggatcgaa
      121 gagcaggcgc tgcccgcggt cggaggccct gagttcttgg agcacgacca ggattctaaa
      181 cgcagcatgg aatcccgcct gaaagacctc aaactcaaca agagcattct tgtcaatacc
      241 cgcacgatct atgaacaacg tctcaaacac atgcttggcc gtagcccggc cgtaccctcc
      301 gggatcaatg tcgagacgat cacctttgac acagtggagc gcctgacgga acagttcggg
      361 ctcgggcagc gcctgattgc cggcgcgctc ttgcaggata gtgcgaatct caagcttgcc
      421 ataaaacagg ctgcatccat gacggggacc cagttgacga tcgcgatgcc gcaggtctcg
      481 gtggacatcg cccgcacggt cggatattac gcactcggtg acaaatttac cgtggagctg
      541 atccgcgcgg atttcaattt cagccttgac cgtctccgcg gcgagaaaat gagggacgaa
      601 caggttgatg cagagatctt ccagaacgtc ggcaagatca aggacctcca ggacgagcag
      661 gccgtgctcc gtcgtatgct ggaacaggag aaagcgctgt tgaaagagct tcgcgagatc
      721 cgtgagaacc gggcgaagct cctagccgag atcgaatcgc agcccgaatt ggtccgttac
      781 cgcgaccggc agtgggtgac ggcggtcact gagcttgaga acgcgcggga taaggaaacg
      841 ggcgcggagc tcggggttag tctccttgaa gcccgattga tggatcccga cgggttccag
      901 gaagcggtcc gcgtgagcct tgttaatatg aaggaagcat tacccgaaga gaagcacgcc
      961 gtgctggcca gggctcttga aaatttcgga ttgaatgaag acggatcccg tgtggcactc
     1021 gtgtcagatg aggtgctgac ggcccgcgcg cgagaactgg ccaagctggt cctgcctaaa
     1081 ggatccaccg attttaaacc ggccccgttg gagcgggtga tggacgcgat tttgctttcg
     1141 atgcgctacc ggctgcagac cttcgtcgaa aaagcggaag cggatgaact agatgccgag
     1201 gctatgaatg tttttagtct ttatgcgacg gccgcgttag gctacgggtt tggccaggaa
     1261 agttacgggg atgt
//
LOCUS       DAOOBX010000284         1266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_117158_length_1266_cov_4.137903, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000284 DAOOBX010000000
VERSION     DAOOBX010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117158_length_1266_cov_4.137903"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1266)
                     /locus_tag="PLY30_04245"
     CDS             complement(<1..>1266)
                     /locus_tag="PLY30_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPW65351.1"
                     /translation="SAARRFTTLLEEIRMNASRVGAHAVLFDSFADQFLGLVELDVYS
                     LHERDKNRVQIYDVSLARQKEYRVVFVAGMLERVFPVQIREDPLLSDWERAIMNAGLE
                     HPLQERLPRQSLERYLFYLAVTRASERLYFSYPHIDLEGKESLRSFYLEEVQRLFDGK
                     VPVVKQKLARPYPGAEEAITGREREVAFLGELRERVESGGKIPAEGPPARVPLDKLLR
                     AITPIEAVIGDPGIRNGGFFDVGEASPTRLEEFAKCPYRYFAHHILNLQDPTEDITSR
                     QKGNILHWVLEEYFRDFIRTGNHHAAPEKFVEEKLKEAFQRYPLTAEQKFREDLDRAD
                     VKEMLLSVLEKEIEKLSVRQLRPAYVEYGFGTGKDYRSPAFRIRDGEKEIRIRGRIDR
                     IDTDGEGKAGLVVDYKRTAKLDREALAIG"
BASE COUNT          248 a          392 c          365 g          261 t
ORIGIN      
        1 tgccgatcgc gagggcctcg cggtccagtt tcgccgttcg cttgtaatcc acgaccaggc
       61 ccgccttccc ttccccatcc gtgtcgatcc gatcgattcg gccccgaatc cggatttcct
      121 tttcgccgtc ccggatccgg aacgccggag accggtagtc ctttcccgtc ccgaaaccgt
      181 attcgacata cgccggacgg agttggcgga ccgaaagttt ctcgatctcc ttttcgagga
      241 ccgaaaggag catctccttc acgtcggccc ggtccagatc ctcccggaat ttctgttccg
      301 ccgtgagggg gtatctctga aaagcctctt tcagtttttc ctcgacaaac ttttcaggcg
      361 ccgcgtggtg atttcccgtc cggatgaagt ctcggaaata ttcctcgagc acccaatgca
      421 ggatgttccc cttctgccgg gaagtaatgt cttccgtcgg gtcctggaga ttgaggatgt
      481 gatgcgcgaa ataacggtaa ggacatttcg cgaattcctc gaggcgggtc gggctggcct
      541 ctccgacatc aaaaaaaccg ccgttccgga ttccggggtc cccgatcacg gcctcgatgg
      601 gggtgatcgc ccgcaggagc ttgtcgagag gaacgcgcgc gggcggacct tccgcgggaa
      661 tcttgccgcc ggactcaacg cgctcgcgta actctccgag aaaagcgacc tcgcgttcgc
      721 ggcccgtgat cgcctcctcc gccccgggat agggcctcgc gagcttctgc ttcacgacgg
      781 ggaccttccc gtcaaaaagc ctctggacct cctcgagata aaaggaccgg agcgattcct
      841 tgccttcgag gtcgatgtga gggtacgaga aataaaggcg ctcgctcgcc cgcgtcacgg
      901 cgaggtagaa aagatatcgc tcaagactct gccggggcag gcgttcctgc aacgggtgtt
      961 cgagccctgc gttcatgatc gcgcgttccc agtccgaaag aagcggatcc tcgcgaatct
     1021 gcacgggaaa aacccgctcg agcatcccgg ccacgaaaac aactcggtat tctttttgac
     1081 gagcaagcga cacgtcgtag atctggaccc ggttcttgtc gcgctcgtgg agtgaataca
     1141 catcgagttc gacaagcccg aggaactgat cggcgaagga atcgaacaaa acggcgtgcg
     1201 cgccgactct ggaggcgttc atccggatct cttcaagcag tgtcgtgaaa cgccgcgcgg
     1261 ccgagg
//
LOCUS       DAOOBX010000285         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_117307_length_1265_cov_2.885124, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000285 DAOOBX010000000
VERSION     DAOOBX010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117307_length_1265_cov_2.885124"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1265
                     /locus_tag="PLY30_04250"
     CDS             <1..>1265
                     /locus_tag="PLY30_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65352.1"
                     /translation="PRISAIDTRREAALYDAALREAPLAIEKVLSEAYLQPKSSVLND
                     PRYQLQPPRGTPTLAELREGDPKRLSFARAEVRMTAIASIAEAEDATAAMERVRAAIP
                     TLGPVARPFVENELSKFNHAYMDRAENVIWMFNGPETFALVVRDAAKRNAPKREVLLS
                     GARGATQTYQLGDLSVVYYPKIDRDTAKIPQWILAQYYSQAGISTTTVGGGVTATMGQ
                     LHAAKPSAPAVKDAAAPQLERRIKDNLAVLNAYLEANFSKDACVTLDPGRILTPGNDG
                     QAKAVRTILLDEAFGAKLEVLSGMAANARSALTHAVALKLLRYSRSLDAFRTAVATAE
                     WLAVQGIIVNYANILTYSEAGKREAFLKSKYSAEKDADDWMRIAEEYFTDKQITTAEG
                     ALKEAELLIAKNDAARLERLAALKQKISG"
BASE COUNT          276 a          362 c          368 g          259 t
ORIGIN      
        1 ccccgcgcat ttccgcgatc gacaccagga gagaggctgc cttgtatgac gcggctcttc
       61 gcgaagcccc tcttgccata gagaaggtcc tctccgaagc ttatcttcag ccaaagtcat
      121 ccgtgctgaa cgatccgcgc tatcaattgc agccaccccg cggaactccg acgctcgccg
      181 aactccgtga gggcgatccc aagcgccttt cgttcgcccg cgccgaggtc aggatgaccg
      241 ccatcgcaag catcgcggaa gcagaggatg ccacagcggc catggaacgg gtccgggctg
      301 ccattccgac cctcggtcct gttgcgagac cctttgtcga aaacgagctt tcaaagttta
      361 atcatgctta tatggatagg gctgagaacg tgatctggat gttcaacggt cctgaaacct
      421 tcgctttggt tgtcagggat gcggccaagc ggaatgctcc taaacgggaa gttctgctct
      481 ccggagctcg tggcgcgact caaacctatc agcttggcga tctgtcggtg gtctattatc
      541 cgaagatcga tcgggatacg gcgaagattc ctcagtggat tctggctcaa tattactctc
      601 aagccggtat ttccacgacg acggtcggcg ggggcgtcac cgcgacgatg ggacagttac
      661 atgcggcgaa accttccgcc cctgcggtta aggacgccgc cgcgccccaa cttgagcgtc
      721 gtattaagga taaccttgcg gtgctgaacg cgtatctcga ggctaatttt tcgaaggacg
      781 cctgcgtcac actggatccg ggacgcatcc tgacgcccgg caatgatggg caggcgaaag
      841 cggtccggac gatccttctt gatgaggctt tcggcgcaaa actcgaggtt ttgtccggta
      901 tggccgcgaa cgcgcggagc gccttgaccc atgctgtcgc tttgaagctg ctccggtatt
      961 ccagaagtct ggacgccttc aggaccgcgg tcgcgacggc ggaatggctc gccgttcagg
     1021 ggatcattgt caattatgcg aacatcctga cctattccga agccggaaag agggaagcgt
     1081 tcctcaagtc aaaatattcg gcggaaaaag acgccgatga ctggatgcgg atcgcggaag
     1141 aatatttcac ggacaaacag atcacaacgg ctgaaggcgc gctcaaggaa gccgaacttt
     1201 tgatcgcgaa aaatgacgcc gcgcgtctcg aacggcttgc cgcgctgaag cagaagatct
     1261 ccggg
//
LOCUS       DAOOBX010000286         1263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_117560_length_1263_cov_3.467715, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000286 DAOOBX010000000
VERSION     DAOOBX010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117560_length_1263_cov_3.467715"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PLY30_04255"
     CDS             <1..174
                     /locus_tag="PLY30_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L16"
                     /protein_id="HPW65353.1"
                     /translation="RMGKGKGAPEYYVAVIKPGRILFEMGGVPEQLARSAMRLCAHKL
                     PFRTKFVKREAAG"
     gene            176..382
                     /gene="rpmC"
                     /locus_tag="PLY30_04260"
     CDS             176..382
                     /gene="rpmC"
                     /locus_tag="PLY30_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPW65354.1"
                     /translation="MLKTSELRELGIEELQTKVLNLKKDLMQLRFQQKTGKLERQNAL
                     SQTKRDIARILTVINETKKAGKKK"
     gene            379..648
                     /gene="rpsQ"
                     /locus_tag="PLY30_04265"
     CDS             379..648
                     /gene="rpsQ"
                     /locus_tag="PLY30_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPW65355.1"
                     /translation="MSEQTEQTKTESSKRVREGVVVSNKMNKTIIVSVTRLLRHPKFN
                     KIIRRQIHYAAHDEKNLAREGDRVQIVESKPLSKTKRWRLVKVIS"
     gene            645..1013
                     /gene="rplN"
                     /locus_tag="PLY30_04270"
     CDS             645..1013
                     /gene="rplN"
                     /locus_tag="PLY30_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPW65356.1"
                     /translation="MIQMRSVLEIADNSGAKKAAMIGVLGRHGKRTAEVGDIITASVK
                     EALPNGAIKKGEVVQAVLIRTKSPIRRRDGSTVRFSSNAIVLIDNQKNPRGTRIFGPV
                     ARELRDADFTKIISLAPEVV"
     gene            1017..>1263
                     /gene="rplX"
                     /locus_tag="PLY30_04275"
     CDS             1017..>1263
                     /gene="rplX"
                     /locus_tag="PLY30_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPW65357.1"
                     /translation="MKIIKGDEVIVTKGKDKGKKGRVMRVYPKENMLLIEKINYRTVF
                     LRKSQDNPKGGITKVEGKLSVSNVKLVCPRSGKPTRVG"
BASE COUNT          324 a          322 c          389 g          228 t
ORIGIN      
        1 cgcatgggta aaggaaaagg cgccccggaa tactatgtgg ctgtgatcaa gcccggccgt
       61 attctttttg agatgggcgg cgtgccggag cagctcgcaa gatcggcgat gcggctttgc
      121 gcgcacaagc ttccgttccg taccaaattt gtcaagcgcg aggcagcggg ctgatatgct
      181 taaaacttcc gaactccgtg aattgggcat cgaggaactt cagacgaaag tcctgaatct
      241 caagaaggac ctgatgcagc tccgtttcca gcagaagaca gggaagctcg agcgccagaa
      301 tgcgttaagt cagacgaagc gtgacatcgc gcggattctg acggtcatca acgagaccaa
      361 aaaggccggg aaaaagaaat gagcgaacag accgaacaga caaagaccga atccagcaaa
      421 cgcgtccgcg agggcgtggt cgtcagcaac aagatgaaca agacgatcat cgtttccgtg
      481 acgcgtctcc tgcggcatcc gaagtttaac aagatcatca gacgtcagat ccattacgcg
      541 gctcacgacg aaaagaacct ggctagggaa ggtgaccggg tccagattgt ggaatcgaag
      601 ccgctttcca agacgaagcg ctggcgcctc gtgaaggtga tatcatgatc cagatgcgaa
      661 gcgttctcga gatcgccgat aacagcggcg cgaaaaaggc ggccatgatc ggtgtgttgg
      721 gccgtcatgg caagcgcact gccgaggtgg gggatatcat cacagcctcc gttaaggaag
      781 cgttgccgaa cggcgccatc aagaagggcg aggttgtcca ggcggttctc ataaggacca
      841 agagtccgat ccgcaggcgg gacgggtcca ccgtccgttt ttcgtcgaac gcgatcgtac
      901 tgatcgacaa tcagaagaac ccgcgcggga cgcgtatctt cggccctgtg gcccgcgagc
      961 ttcgcgatgc ggatttcacg aagatcattt ctttggctcc ggaggtcgtt taagtcatga
     1021 agatcatcaa gggcgatgag gtcatcgtta cgaaggggaa ggacaagggc aagaaaggcc
     1081 gcgtgatgcg ggtgtatccg aaggagaata tgctcctgat cgagaagatc aactaccgga
     1141 cggtgttcct ccgcaagagc caggacaacc cgaagggcgg catcaccaag gttgaaggta
     1201 agctgtcggt gtccaatgtc aagctggtgt gtccccgcag cggcaagccc acccgggtcg
     1261 gat
//
LOCUS       DAOOBX010000287         1263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_117593_length_1263_cov_2.645695, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000287 DAOOBX010000000
VERSION     DAOOBX010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117593_length_1263_cov_2.645695"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /gene="proB"
                     /locus_tag="PLY30_04280"
     CDS             <1..1052
                     /gene="proB"
                     /locus_tag="PLY30_04280"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPW65358.1"
                     /translation="RESFSSAEHRRVCGEILALIRMKKRPVVVSSGAIAYGMKSCGLK
                     KRPKEMAGLQACAAIGQGKLIHAYESYFSKKKIQTAQVLLTRDGLEDRKRFLCSRNAM
                     QKLLEMNVLPIVNENDTVAVEEIAFGDNDILSVHVAHLVGADLLINLSDADGFLLKDG
                     TRVREVASEDEIDNKLLMHLSEKKREETVGGMRAKLVAARVAMRLGIPFMIVNGHKPG
                     ILRDILAGRDIGTLFMPSGVKKDQRHKWIAFSAHRYGMLTVDAGAWQALLVGHKSLLP
                     GGITRVQGDFREGQVVELQTEDGEVFGRGIVKYGSRDLALIAGKRTDKIAGLLGRKAS
                     AEVIHRNDLVLWGGR"
     gene            1049..>1263
                     /locus_tag="PLY30_04285"
     CDS             1049..>1263
                     /locus_tag="PLY30_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65359.1"
                     /translation="MKSSGLRELDVMVGKVRDASREAAQWSGDFKKKLLLDVSRRILS
                     QKKYLLRENEKEVHRARAAGLAPAMVDR"
BASE COUNT          274 a          360 c          395 g          234 t
ORIGIN      
        1 gccgtgagag tttctcttcc gccgaacacc ggcgcgtctg cggtgagatc ctcgcgctga
       61 tccgcatgaa aaaacggccc gtcgtcgtga gctccggtgc catcgcgtac gggatgaaat
      121 cctgcggcct caagaagcgc ccgaaggaaa tggccggact tcaggcctgc gcggcgatag
      181 gacagggaaa gttgatccac gcctacgaaa gctatttctc gaagaaaaag atccagacgg
      241 cccaggtcct tctgacccgg gacgggctcg aggatcgcaa acgtttcctt tgctcccgca
      301 acgccatgca gaagctcctc gagatgaacg tcctgcccat cgtcaacgag aacgacaccg
      361 tcgcggtcga ggagatcgcg ttcggggaca acgacatcct ctcggtccat gtggcccatc
      421 tggtcggcgc ggaccttctg ataaatcttt ccgacgcgga cgggttcctc ctgaaggacg
      481 ggacacgggt ccgtgaagtg gcctccgagg acgagatcga caacaagttg ctcatgcatc
      541 tttcagaaaa aaagcgcgaa gagaccgtcg gcgggatgcg cgcgaagctg gtcgcggcgc
      601 gcgtcgccat gagactcggc attccgttca tgattgtcaa cggtcataag cccgggattc
      661 tccgggatat tctcgccggc agggatatcg ggacgctttt catgccgtcc ggcgtcaaaa
      721 aggaccagcg ccacaaatgg atcgcgttct cggcgcaccg ttatgggatg ctgaccgtcg
      781 acgccggtgc gtggcaagcg cttctggtcg gtcataagag cctgcttccg ggcgggatta
      841 cccgagtcca gggtgatttc agggaaggac aggtcgtcga gctccagacc gaagacggcg
      901 aggtgttcgg ccgcggcatc gtgaaatatg gaagccggga ccttgccctg atcgcgggaa
      961 aacggacgga caagatcgcg ggactgcttg gccgtaaggc ttccgcagaa gtcattcacc
     1021 gcaatgatct tgttctgtgg ggtggccgat gaagagctcc ggcctccggg aactcgacgt
     1081 tatggtcgga aaggtccgcg acgcttctcg ggaagccgcg cagtggagcg gtgatttcaa
     1141 aaaaaagctt ctgctcgacg tatcgagacg gattctttcg cagaagaaat acctcctccg
     1201 cgaaaacgaa aaagaagttc atcgagcccg cgccgcaggg ctggccccgg ccatggttga
     1261 ccg
//
LOCUS       DAOOBX010000288         1260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_118036_length_1260_cov_2.325311, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000288 DAOOBX010000000
VERSION     DAOOBX010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118036_length_1260_cov_2.325311"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PLY30_04290"
     CDS             complement(<1..1235)
                     /locus_tag="PLY30_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPW65360.1"
                     /translation="MDTWFSSWLWPFSTLGWPENTGDLKKFYPTSDLITAPEIIFFWV
                     ARMIMAGLEFMGEVPFTRINIHGTVRAASGLKMSKSLGNSIDPLEVINEMGADALRYS
                     MIMLSAQDVYLSREKFEVGRNFTNKVWNACRFVLRSLEDFKEGSVDPEQFKGMELSLP
                     SRWILSRLEEKTAEIERHLSQFGLAQATSELYHFVWDDFCDWYIELAKTMIQGRDEAL
                     KLQTRKVLFFVCERILRLLHPFMPFVTEEVWHKFKEKAADGVSWPESIMLAAWPHDEG
                     AEKFRDPGAQRAVSFLQDAVRGIRDLRARFQIAPANKLKAVIACKEKAALEILRCFER
                     EVKSLARLESLECVSQFKKQGAMVASSFADFDVYILVGGILDLAEEKKRVQKKIAETE
                     IWIQGTRKNWITPFLASRPP"
BASE COUNT          284 a          365 c          331 g          280 t
ORIGIN      
        1 gggggccggg aagccaaaaa aggggttatc cagtttttac gggtcccctg gatccagatc
       61 tccgtttccg cgatcttctt ttgaacgcgc ttcttttcct cggccagatc cagtatcccg
      121 ccgaccagga tgtaaacatc gaaatccgca aaagagcttg cgaccatcgc gccctgtttt
      181 ttgaactgcg atacgcattc gagcgattcg aggcgggcga gcgatttgac ctcgcgctca
      241 aaacagcgca ggatctcaag agccgctttt tccttgcatg cgatcaccgc tttcagtttg
      301 ttcgccggcg cgatttgaaa cctcgcccgg agatcccgga tccctctcac cgcgtcctgg
      361 aggaacgaaa cggcccgctg agctccgggg tcccggaact tttccgcccc ctcgtcgtga
      421 ggccatgcgg ccagcataat actttccggc cacgaaaccc cgtcggccgc cttctccttg
      481 aatttgtgcc aaacctcttc ggtaacaaaa ggcatgaagg ggtggagaag acggaggatc
      541 cgttcacaaa cgaaaaagag gaccttccgg gtttgtagtt tcaatgcctc atccctgccc
      601 tggatcatgg tctttgccaa ttcgatatac cagtcgcaaa aatcatccca cacgaaatga
      661 tagagttcgc tcgtcgcctg tgcgaggccg aactgggaga gatgtcgttc gatctcggcg
      721 gtcttctctt caagcctcga aaggatccac cggctcggaa gagagagctc catgcccttg
      781 aactgctccg gatcaaccga tccttcttta aaatcttcca atgagcgaag cacgaaccgg
      841 caggcgttcc agaccttgtt cgtgaaatta cggccgactt cgaatttctc gcgcgacaga
      901 tagacatcct gcgccgaaag catgatcatg ctgtagcgga gcgcgtcggc gcccatctca
      961 ttgatcacct ccaacgggtc gatcgaattg ccgagggatt tcgacatctt gagtccgctt
     1021 gccgcgcgga ccgtcccgtg aatgttgatg cgagtgaacg gaacttctcc catgaattcg
     1081 agcccggcca tgatcatgcg ggcgacccag aaaaaaatga tctcgggagc ggtaatgagg
     1141 tcggaggtcg gatagaactt tttaagatcc ccggtgttct cgggccagcc aagcgtggaa
     1201 aacggccaaa gccagcttga gaaccaggtg tccaatacgt ccggatcctg tttaagatcc
//
LOCUS       DAOOBX010000289         1259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_118107_length_1259_cov_3.892027, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000289 DAOOBX010000000
VERSION     DAOOBX010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118107_length_1259_cov_3.892027"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1259
                     /gene="proS"
                     /locus_tag="PLY30_04295"
     CDS             <1..>1259
                     /gene="proS"
                     /locus_tag="PLY30_04295"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPW65361.1"
                     /translation="PLGFRVLLKIIGIIREEMNRAGACEVLMPALHPAELWKKSGRYE
                     TLGEDKIVFKNRAGHEFVLGPTHEEVITDLVGTNIKSFRDLPLNLYQIQNKFRDEARP
                     RFGVIRTKEFIMKDAYSFDRDEAGLDVNYRKMSEAYHRIFSRCGLQFQVVSADTGMMG
                     GKMSQEFMVICPYGEDRVASSGSGYLASQDIAERGDPGLKTPVPQSLAPEKFDTPNLR
                     TIEEITHKYKVPAHQMIKTLIFMADDKPVAALVTGENEVNEGKLRKLIGAKTIRQAEA
                     HEIRDATGAPVGFAGPIGLKIPVYADWDVIGIADGVTGANEEDKHFRNVTYGRDFTPT
                     ATGDIRFVKDGDRAPDGSGTLKLTTSMEIGHIFKLGTRYSESVGAHFLDEKGERKPVI
                     MGCYGIGVNRILASAIEQNHDAKGIKW"
BASE COUNT          275 a          359 c          414 g          211 t
ORIGIN      
        1 cccgctcggt ttccgcgtgc ttctcaagat catcgggatc attcgcgagg agatgaaccg
       61 ggccggcgcc tgcgaggtcc tgatgcccgc gctgcatccc gcggagctct ggaagaagtc
      121 cggccgttac gaaacgctcg gcgaggacaa gatcgtgttc aagaaccgtg cggggcacga
      181 gttcgtgctc ggcccgaccc atgaggaggt catcacggac cttgtcggga cgaacatcaa
      241 atcctttcgc gatcttccgc tcaacctgta ccagatccag aacaaattcc gcgacgaggc
      301 ccggccgagg ttcggggtga tccgcacgaa ggaattcatt atgaaggacg cctacagctt
      361 tgaccgcgat gaggcggggc tcgatgtcaa ttaccggaag atgtcggagg cttatcaccg
      421 tatcttcagc cgttgcgggc tccagttcca ggtcgtgagc gcggacaccg gtatgatggg
      481 cggcaagatg agccaggagt tcatggtgat ctgtccgtac ggcgaggacc gcgtcgcgag
      541 ttccgggtcc gggtatctcg cgagccagga tattgccgag cgcggcgacc cgggattgaa
      601 aacgccggtc ccgcagagtc tcgcccccga aaagttcgac acgccgaatc tgcggacgat
      661 cgaggagatc acgcataagt acaaggtccc cgcgcaccag atgatcaaga ccctgatttt
      721 catggcggac gacaagccgg tggcggccct cgtgacgggc gagaatgaag tgaacgaagg
      781 aaaactccgg aagctcatcg gcgccaaaac gatccggcag gcggaagcgc acgagatccg
      841 cgatgcgacg ggcgctccgg tcgggttcgc gggtccgatc ggcctcaaga ttcccgtgta
      901 cgcggactgg gacgtgatcg ggatcgcgga cggagtgacg ggcgcgaacg aggaggacaa
      961 gcatttcagg aacgtgacct acggccgtga tttcacgccg acggcaacgg gcgatatccg
     1021 tttcgtcaag gacggcgatc gcgcgccgga cggaagcgga acgctcaagc tcacgaccag
     1081 catggagatc ggacacatct tcaagctcgg cacgcggtat tcggaatcgg tcggggcgca
     1141 cttccttgac gaaaagggcg aacgcaagcc cgtgatcatg ggctgctacg ggatcggggt
     1201 gaaccgcatt cttgcgtccg cgatcgagca gaaccatgat gcgaaaggca tcaagtggc
//
LOCUS       DAOOBX010000290         1253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_118929_length_1253_cov_2.528381, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000290 DAOOBX010000000
VERSION     DAOOBX010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118929_length_1253_cov_2.528381"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..>1253
                     /locus_tag="PLY30_04300"
     CDS             294..>1253
                     /locus_tag="PLY30_04300"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HPW65362.1"
                     /translation="MRKIIPYLIPALVLTGILFLLYQFPDQIEDFRIMVFDNYQRIRP
                     RIYKDTPVRIIDIDDKSLERFGQWPWPRTVLAKLVTRLSKAGAQTIAFDVVFAEPDRT
                     SPKNVIRSWPDVPALQAVRSQIDVMPDHDLLFAEAADKARVVFAFGLIAAPDRNIPAV
                     KYGGFLEHGAPGDRTIDYIGPAYKGAVTNIPSLEKAARGLGGFNISSDEKGMIRRAPA
                     LFRLRDTIYPSLSLEAMRVFQEVNVFKVSLAGTETNASFGEKTGIVSVKTGFLDIPTD
                     KRGRIWLYDTGHRPERFIPAWKVFSDDFDAASIDGKLLFVGTSA"
BASE COUNT          267 a          381 c          340 g          265 t
ORIGIN      
        1 ggacaagctg caaaagggtc tgaagaaaga aatcgatcag gatgcttttg tccgtgatcg
       61 cgtcataaat cctgccgatc tcgtgcgtgg ctttgtctgg attaccggaa tgcgacatgg
      121 ggcgatacta ataaaagctt tcggtgtttg caagcgtctg aatgcccgac ctcacgcggg
      181 gacctggaaa tcgcaaatac atttggtgaa tgagtcccgg gaaaccggtc ccggatttcg
      241 ggcgtcggca ggcaagcatt gcaccctgta tgaaaacagc tataatccct cccatgcgga
      301 agatcatccc ctatctcata cccgccctcg tcctcaccgg cattcttttc ctcctttatc
      361 agttccccga ccagatcgag gacttccgca tcatggtctt cgacaattat caacgcatcc
      421 gtccccgcat ctacaaggac actccggtcc gcatcatcga tatcgatgat aaaagcctcg
      481 aaaggttcgg tcagtggccg tggccgcgca cggtactcgc caaactcgtc acgcgactct
      541 ctaaggccgg cgctcaaacg atcgccttcg acgtcgtgtt cgccgaaccc gatcgcacct
      601 caccgaagaa cgtgatcaga tcatggcctg acgtaccggc cctgcaggcg gtgcggtccc
      661 agatcgacgt gatgccggac cacgacctgc ttttcgcgga agccgcggac aaagccaggg
      721 tcgttttcgc tttcggtctg atcgccgcgc ccgacaggaa catcccggcc gtcaaatacg
      781 gtggtttcct ggaacacggt gctccgggag atcggaccat tgattatatc ggccccgctt
      841 acaagggcgc ggtcacgaat atcccctctc tcgaaaaagc ggccaggggc ctcggcggat
      901 tcaatatcag ctccgatgaa aaagggatga tccgtcgcgc gcccgctctc ttccgtctga
      961 gggacacgat ctatccgtcg ctgtcgcttg aggccatgcg ggtttttcag gaggtgaacg
     1021 tgttcaaggt ctcgctcgca ggtacggaaa cgaacgcctc gttcggcgag aagaccggca
     1081 tcgtctccgt caaaacggga tttctcgaca tcccgaccga caaacgcgga cggatctggc
     1141 tctatgacac ggggcaccgc cccgagcggt tcatcccggc ctggaaggtc ttctccgacg
     1201 attttgatgc ggcatccatc gatggcaagc tgctcttcgt cgggacgagc gcg
//
LOCUS       DAOOBX010000291         1251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_119177_length_1251_cov_3.857860, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000291 DAOOBX010000000
VERSION     DAOOBX010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119177_length_1251_cov_3.857860"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLY30_04305"
     CDS             <1..195
                     /locus_tag="PLY30_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65363.1"
                     /translation="LGTRQSGVPFFRVADLLTDEAMLILARDRARTIVDALDLDALKN
                     PGHWIHKYLGERNVSLPHSS"
     gene            355..954
                     /gene="rsmD"
                     /locus_tag="PLY30_04310"
     CDS             355..954
                     /gene="rsmD"
                     /locus_tag="PLY30_04310"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPW65364.1"
                     /translation="MRIISGKFKSRLIDFPKSKLTRPMTDRTKETLFNIVGSFVFGKH
                     ILDLYAGSGSIGLEALSRGALSATFVDRAPWAKSSILKNLAALGLEREGLLLSMDVLA
                     GIRKLDRTGRAFSLVFVDPPFDKGLVRKTLLKLDASEIVQPFGQVVVGHSNREAIPHE
                     ELTKLKLVRTKKIGQASLSFLFRLESANEKTKSYISGEL"
     gene            917..>1251
                     /gene="coaD"
                     /locus_tag="PLY30_04315"
     CDS             917..>1251
                     /gene="coaD"
                     /locus_tag="PLY30_04315"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPW65365.1"
                     /translation="MKKRKAIYPGSFDPVTFGHLDLIERALELFDELVVVIASNPAKK
                     TLFTTKERVFFIKNAVKRFPNVSVACWKGLTVDFAKRSGASTIIRGIRATSDFDYEFQ
                     MALTNRQLAP"
BASE COUNT          275 a          386 c          341 g          249 t
ORIGIN      
        1 ctcggcaccc ggcagtcggg ggtcccgttc ttccgggtcg cggaccttct gaccgatgag
       61 gccatgctca ttctcgcccg ggaccgcgcc cgcaccatcg tggacgccct cgacctcgat
      121 gctctgaaaa atcccggcca ctggatacac aaatatctgg gagaacggaa cgtttctcta
      181 ccccactcat cctgagcctc cagaaggccg ggctgaaggg tctcatgaac acgattcttc
      241 gacttcgcct cacagcacgg ggcaggcgca ggacaactcc ctcgggggaa ataccccgtg
      301 ggatcgcaga aatatctcga acaaatgcca aaaagcgatt aaaataccgc tcgcatgcgc
      361 atcatttctg gcaaattcaa atcccggctg attgattttc cgaagagcaa gctgacccgt
      421 cccatgacgg accggaccaa agagaccctc ttcaatatcg tggggtcctt cgtattcggg
      481 aaacacatcc tggacctgta tgccggatcg ggcagtatcg gccttgaggc cctgagccgg
      541 ggagcgcttt cggcgacctt tgtcgaccgg gcgccttggg cgaaaagctc catcctgaag
      601 aacctcgcgg cgctggggct tgaaagggaa gggttgctgc tttcgatgga cgtgctcgca
      661 ggaatccgca aactcgacag gacgggacgg gcgttctccc tcgtcttcgt ggacccgccg
      721 ttcgacaagg ggctcgtccg gaaaacgctg ctcaagctcg acgcctcgga gatcgtccag
      781 ccgttcggtc aggtcgtggt tggccattcg aatcgcgagg cgattcctca cgaagaactc
      841 acaaaactga aactggtacg caccaaaaag atcggacagg caagtctctc gttcctgttc
      901 cgtctggaat ccgccaatga aaaaacgaaa agctatatat ccggggagct ttgatcccgt
      961 cacgttcggg catctggacc tgatcgaacg cgcgctcgaa ctcttcgacg aactggtcgt
     1021 cgtaatcgcc tccaatcccg cgaagaagac ccttttcacc acgaaggaac gcgtgttttt
     1081 catcaagaat gccgtcaagc ggttcccgaa cgtctccgtc gcgtgctgga agggactcac
     1141 cgtggatttt gccaaacgct ccggagcctc cacgattatc cgcggcatcc gggccacgtc
     1201 cgatttcgat tacgagttcc agatggcgct gacgaaccgc cagctcgcgc c
//
LOCUS       DAOOBX010000292         1244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_120139_length_1244_cov_2.957948, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000292 DAOOBX010000000
VERSION     DAOOBX010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120139_length_1244_cov_2.957948"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLY30_04320"
     CDS             <1..190
                     /locus_tag="PLY30_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65366.1"
                     /translation="PPFIADHFSEKAGADACLEIVEVPGASHQEGWTAAWPRLLNEPF
                     SSCPESSPTPPPGARLRG"
     gene            305..>1244
                     /locus_tag="PLY30_04325"
     CDS             305..>1244
                     /locus_tag="PLY30_04325"
                     /inference="COORDINATES: protein motif:HMM:NF020086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPW65367.1"
                     /translation="MANAVSDRARFLPIDRNDLKARGWTECDIILITGDAYVDHPSYG
                     TALIGRALEAGGYRVGVIAQPDWRKADDFLALGRPRLFFGISAGNLDSMVSNYTPSRK
                     PRRTDDYSPGGKSGFRPNRATIVYTSKVKGLFLGVPVVIGGIEASLRRLAHYDYWEDK
                     VRRSLLIDTKAEILAYGMAERQMVEIAKRLSMSATPGVDTRGQRPALQWALDGIPGTV
                     VVRRGVPSHGAAKGENAVEVPSFEEVSKDKEKFNLAFKLAYLESDPVRGRTIAQRHGD
                     RFVIQYPPAAPLSTEEMDRIYALPFAYAWHPVYDRAG"
BASE COUNT          230 a          368 c          395 g          251 t
ORIGIN      
        1 gccgcccttc atcgcggatc atttctccga aaaagcggga gcggacgcct gcctcgagat
       61 cgtcgaggtc cccggcgcga gccatcagga aggctggacc gccgcctggc cgcgtctgtt
      121 gaatgagcct ttcagctctt gtcccgaatc atcacccact ccacccccgg gagctagact
      181 ccgggggtag agtgggtata atcctccgcg gggaggcggc aggttcgtga gaagctcggg
      241 tttttgcttg gcaataaatc catgaagagt ctcgtgacat atctgttccc ggcaggtgga
      301 cgtaatggcg aatgcggttt ctgatagagc aaggtttctc ccgatcgatc gaaatgatct
      361 caaggcccgc ggctggacgg aatgcgatat catcctgatc acgggcgacg cctacgtgga
      421 ccatccctcc tacggcacgg cgcttatcgg tcgcgcgctt gaagccgggg gctaccgggt
      481 cggcgtgatc gcgcagccgg actggagaaa ggcggatgat tttctcgcgc tcggccgccc
      541 gcgtcttttc ttcgggatct cggccggcaa tctcgattcc atggtctcga attacacgcc
      601 ctccagaaaa ccgcggcgca ccgacgacta ctccccgggc gggaagagcg ggttccggcc
      661 gaatcgcgcg acgatcgtct acacaagcaa ggtgaaggga cttttccttg gcgtgccggt
      721 tgtgatcggg gggatcgagg cgagcctgcg ccgcctcgcg cattatgatt attgggagga
      781 caaggtccga cgctcccttc tgatcgacac aaaggccgag attctcgcct acgggatggc
      841 cgagcgacag atggtcgaga tcgcgaagcg tctttcaatg tccgcgacac cgggcgtcga
      901 tacccggggt caacgcccgg cgttgcaatg ggcgctggac gggatccccg gcaccgtggt
      961 cgtgcgtcgc ggtgttcctt cgcatggcgc cgcaaagggt gagaacgcgg tggaggttcc
     1021 ttcgtttgag gaagtttcaa aggacaaaga aaaattcaat ctcgcgttca agctcgctta
     1081 tcttgagtcg gaccccgttc gcggccggac gatcgcgcag aggcacgggg accgctttgt
     1141 gatccagtac ccgcccgcgg ctccgctttc cacggaggaa atggatcgta tttacgcact
     1201 gcctttcgcc tacgcctggc atccggtgta tgaccgggcc ggtg
//
LOCUS       DAOOBX010000293         1233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_121599_length_1233_cov_3.622241, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000293 DAOOBX010000000
VERSION     DAOOBX010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121599_length_1233_cov_3.622241"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLY30_04330"
     CDS             <1..279
                     /locus_tag="PLY30_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPW65368.1"
                     /translation="EVIAIGPVIMMKFVSLLTRKYNLKTSVSLNPVMIDGTGMCGGCR
                     VTVGGETKFACVDGPDFDGHLVDYDELMRRQGMYKTEEAHACKIGLKG"
     gene            281..>1233
                     /locus_tag="PLY30_04335"
     CDS             281..>1233
                     /locus_tag="PLY30_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW65369.1"
                     /translation="MAPNMSPRKVPIPEQKPEERVKNFNEVALGYTPEQAKEEATRCL
                     QCKHRPCVAGCPVNVLIPEFIKKVSEGDFDEAYRVLTTENSLPAICGRVCPQETQCEA
                     LCVRGKKGEPVGIGRLERFVADYAMEKRSGEVPAIEKNGIRVAIAGGGPAGLTCAGEL
                     ARLGYDVTIFEALHALGGVLMYGIPEFRLPKKLVEKEIAGVLKLGVKIEKNVIIGKSI
                     TTDELFGDGFKAVFVGCGAGLPSFMKIEGENLNGVYSANEFLTRVNLMKAYDFPRSPT
                     PVRMGKRVAVIGGGDVAMDAARTAKRLGADEVYIVYRRSEK"
BASE COUNT          264 a          342 c          404 g          223 t
ORIGIN      
        1 gaggtcatcg cgatcggccc cgtgattatg atgaagtttg tgagcctcct caccaggaaa
       61 tacaatctca agaccagcgt gtccctcaac cccgtgatga tcgacggcac cgggatgtgc
      121 ggcggatgtc gcgtgacggt cggcggggag acaaagttcg cttgtgtcga cggccccgac
      181 tttgacggcc atctcgtgga ctacgacgaa ttgatgcggc ggcagggtat gtacaagacc
      241 gaagaggccc acgcctgcaa gatcggtctc aagggttgat atggcgccga acatgagccc
      301 caggaaggtt ccgattccgg aacagaaacc tgaagagcgg gtcaagaatt ttaacgaggt
      361 cgcgcttggc tacacgccgg aacaggcgaa ggaagaagcc acgcgttgcc tccagtgcaa
      421 gcaccgtccc tgcgtcgccg gatgccccgt caacgtcctg attcccgagt tcatcaagaa
      481 ggtttcggaa ggcgatttcg acgaagcgta ccgggtcctg acaacggaga acagcctccc
      541 ggccatctgc ggccgcgtgt gtccccagga aacgcagtgc gaggctcttt gcgtgcgcgg
      601 taaaaagggg gagccggtcg ggatcggacg cctcgaacgt tttgttgccg attacgcgat
      661 ggaaaaaagg tccggagagg tccccgccat tgagaagaac ggcatccggg tcgcgatcgc
      721 gggcggcggt ccggcgggac tgacctgtgc gggtgaactc gcgcgcctgg gctatgacgt
      781 gaccatcttt gaggctcttc acgcgctcgg cggcgtactg atgtacggta tccccgagtt
      841 ccggcttccg aaaaaactgg tggaaaagga gatcgcgggc gtgctgaagc tcggcgtcaa
      901 gatcgagaag aacgtgatta tcggaaaatc gatcacgacc gacgagcttt tcggggacgg
      961 tttcaaggcg gtgttcgtcg gttgtggcgc cggccttccc tcgttcatga agatcgaagg
     1021 ggagaacctg aacggcgtct actccgccaa cgaattcctg acccgcgtca atctgatgaa
     1081 ggcgtatgac ttcccgcgtt ccccgactcc cgtcaggatg gggaaacggg tcgcggtcat
     1141 cggcggcggc gacgtggcga tggacgcggc ccggaccgcg aaaaggctcg gcgcggacga
     1201 ggtctacatc gtttaccgcc gcagcgaaaa aga
//
LOCUS       DAOOBX010000294         1225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_122700_length_1225_cov_3.623077, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000294 DAOOBX010000000
VERSION     DAOOBX010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122700_length_1225_cov_3.623077"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1180
                     /gene="rho"
                     /locus_tag="PLY30_04340"
     CDS             113..1180
                     /gene="rho"
                     /locus_tag="PLY30_04340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018917371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPW65370.1"
                     /translation="MRPFDPGNRHGKGSGGERPRRGRPQRPRKPYAPGHGGYRHPAPN
                     GYARNGYARQQPASSQDDEISRKFRASVAIDPSPRLRLEDGLPEMTMRVMDLVSPIGM
                     GQRGLIVAPPGTGKTTFLKHICRSVAKSHPEMKLYCLLIDERPEEVTDFRRSAPAEVR
                     WSSSDESCDHHIATADGLMKQVYREVLDGANVMVLVDSLTRLARVHNAERMSSGRTLS
                     GGVDARALEVPRRIFGSARKIEHGGSLTILATILVDTGSRMDQYIFEEFKGTGNMEIY
                     LSREISNQRIFPAVDISRSGTRKEELLLAPEELEPVRRMRGALAGLKPIESIQALTTQ
                     LRKYPTNRDFLAAVSRELKAG"
BASE COUNT          273 a          342 c          402 g          208 t
ORIGIN      
        1 ccatgcactt ttcaaaggag cgcaatctga agctgaaaga aatccagatc agtatcgaaa
       61 aagccgagga cgaatggtac tccctgcaaa ccaaaatgga taagctgcaa aaatgagacc
      121 ttttgatccg ggaaaccggc acgggaaggg aagcggggga gaacgcccgc ggcgcggccg
      181 gccgcagcgt ccccggaagc cgtatgcgcc gggacacggg ggttaccgtc atcccgcgcc
      241 gaacggctat gcccggaacg gctacgcgcg gcagcagccg gccagttctc aggatgacga
      301 gatctcccgg aaattcaggg cctcggtcgc gatcgatccg tccccgcggc tccgtctcga
      361 ggacggcctc ccggaaatga cgatgcgggt catggacctc gtgtcgccga tcgggatggg
      421 acagcgcggg ctcatcgtcg cgccccccgg taccggcaag acaacttttt taaaacatat
      481 ctgccggtcc gtcgcgaaaa gccatccgga aatgaagctc tactgcctct tgatcgatga
      541 acggccggag gaggtcacgg atttcaggcg gagcgctccg gcggaggtcc gctggtcgtc
      601 ttcggacgaa tcctgcgatc accacatcgc gacggcagac gggctgatga aacaggtcta
      661 ccgcgaggtt ctggacggcg cgaatgtgat ggtgctggtc gattcgctga cgcgccttgc
      721 gcgcgtgcat aacgcggagc ggatgtcgag cgggcgtacg ctttcgggcg gcgtggacgc
      781 gcgggcgctt gaggtgccgc ggcgcatctt cgggtctgct cgcaagatcg agcacggcgg
      841 ttcgctcacg attcttgcga cgatcctggt ggacacaggg agccggatgg atcagtatat
      901 tttcgaagag ttcaaaggga ccgggaatat ggagatctac ctgtcgcgcg agatatcgaa
      961 tcagcgaatc tttccggcgg tcgatatctc caggagcggc acaaggaaag aagaattgct
     1021 tctggccccc gaggaactcg aaccggtgcg aagaatgcgc ggcgccctgg cggggctgaa
     1081 gccgatagag tcgatccagg cgctcacgac gcagctcagg aaatacccga ccaacaggga
     1141 ttttctcgcg gccgtgagtc gtgagcttaa agccggataa cctgcaccaa ctaataccgg
     1201 gagttaagac gggaaaggga ggatg
//
LOCUS       DAOOBX010000295         1225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_122777_length_1225_cov_2.264103, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000295 DAOOBX010000000
VERSION     DAOOBX010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122777_length_1225_cov_2.264103"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1095
                     /locus_tag="PLY30_04345"
     CDS             85..1095
                     /locus_tag="PLY30_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW65371.1"
                     /translation="MMKSLPHKLSRPVRFTLVAIGVFLLMRIFASCGAAKGDLQIVRT
                     AQVVRGDLSVTVSSTGTVQPYNRVEVKAPIAGRIEEVLVREGDEVKKGQVLARMSSVE
                     RAALLDAARSRGDEVVKRWEEAYLPAPLLAPLDGTIIVRSVEPGQTVTTADPVVVLSD
                     RLIVQALVDETDLAFIRLAQPVEITLDAYRDELVPGTVDHIAFESTLENNVNVYAIDV
                     APEALSESFRSGMTANVTFIVKDCKNVLTVPSEAVVAWPKNIPNPSGAVFAVYKKVFG
                     GKLEPVAVKTGETDGVRTEITDGLKEGAEIAVVRKKEKERSSNLFGSPRAARKSGATK
                     SS"
BASE COUNT          261 a          339 c          388 g          237 t
ORIGIN      
        1 aagattatgt cgaaagcctg gccgatgttt ttactcagca ggccaattgg aaaaaaacac
       61 agggcgccac cctggaggat gcttatgatg aaatcgttac cccataaact ttcgcgcccg
      121 gtcaggttca ccctggtcgc gatcggagtg ttcctcctca tgaggatttt tgcctcctgc
      181 ggggcggcga agggggatct ccagattgtc cggaccgccc aggtggttcg cggagatctg
      241 tccgtgacgg tcagttcgac agggacggtc cagccctata atcgcgtcga ggtcaaggcg
      301 ccgatcgcgg gacggatcga ggaagtgctg gtgcgcgaag gggacgaagt gaagaaaggg
      361 caggtgctgg cccgcatgag ctcggtcgag agggcggctc tgctcgatgc cgcgcgttcc
      421 aggggcgacg aggtcgtcaa acggtgggaa gaagcctatc tgcccgcgcc gctccttgcc
      481 ccgctcgacg ggacgatcat tgttcgctcg gtggaacccg gtcaaacggt cacgacggcg
      541 gatccggtgg tggtcctttc cgaccgcctg atcgttcagg ccctggtgga cgagaccgat
      601 ctcgcgttca tccggctcgc tcagccggtg gagatcacgc tggacgccta ccgggacgag
      661 cttgtccccg gtacggtcga ccatatcgcg ttcgaaagca cgctcgaaaa caacgtcaat
      721 gtctatgcga tcgatgtggc cccggaagcg ttgtccgaat cttttcggag cgggatgacc
      781 gcgaacgtta cctttatcgt taaggactgc aaaaatgttc ttacggtccc ctcggaggcg
      841 gtcgtggcct ggccgaagaa catcccgaac ccctccggtg cggtctttgc ggtttacaaa
      901 aaagttttcg gaggcaaact ggaaccggtg gccgtcaaga cgggggaaac cgacggcgtc
      961 aggaccgaga tcacggacgg cctgaaagaa ggcgcggaga tcgcggtggt caggaagaag
     1021 gaaaaggaaa gatcctcgaa tcttttcggc tcgccgcgcg ccgccagaaa aagtggcgcc
     1081 acgaaatcct catgaaactg ctcgaagtcg aaaacatttc caaaacctac aagatcggcg
     1141 aagtcgaggt tcgcgcgctt caggatgttt cgctgacgat ccatgccggc gaattcatcg
     1201 cgatcatggg gccttccggt tccgg
//
LOCUS       DAOOBX010000296         1220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_123420_length_1220_cov_3.127039, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000296 DAOOBX010000000
VERSION     DAOOBX010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123420_length_1220_cov_3.127039"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLY30_04350"
     CDS             complement(<1..307)
                     /locus_tag="PLY30_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386654.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPW65372.1"
                     /translation="MKKTILALITCPHIGNHGINAADKIPVANPDGVSLSDGSGDSAV
                     VRCLVQAVKKLIGKVPIFGVCLGLQSMGVAYGGVVDRAPELRHGKTSPVFHNGTGVFA
                     "
     gene            complement(323..>1220)
                     /locus_tag="PLY30_04355"
     CDS             complement(323..>1220)
                     /locus_tag="PLY30_04355"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPW65373.1"
                     /translation="TQGREGKKVAEMAEMKVSGAVAVTDDGNWVVDSGVARRAYEYAA
                     TYGLPVMTHAEDPTISLNGVMNEGIVSTKLGLRGRPNASEDIAIARDIELTRLIKCHL
                     HVQHVSTCGGVAMIRRAKDEGLPVTAEATPHHLALTDEEFVKYDTFYKMNPPLRTEKD
                     RQAVIRGLEDGTIDAIATDHAPHAFEEKDMDIEVAPFGTIGLESAFGVVLTELYHGRK
                     WKIEDVLRKMSIAPSEILKKTNFGRLKAGEHANFILADLDLEWVFEQKNVYSKSVNSC
                     FFGKKLKGKVLHTFVKGFQYSL"
BASE COUNT          219 a          374 c          345 g          282 t
ORIGIN      
        1 ccgcgaacac gcccgtaccg ttgtggaaga ccgggctcgt cttgccgtgc cgcagctcgg
       61 gggcgcggtc gacgacgccg ccgtacgcga cgcccatcga ctgaaggccg aggcagacgc
      121 cgaagatcgg gaccttgccg atcaatttct ttacagcctg aacaagacac ctcacaaccg
      181 cggaatcgcc ggaaccgtcc gagagggaaa ccccgtccgg gttcgcgacc gggatcttat
      241 ccgccgcgtt gattccgtgg ttaccgatat gcgggcaggt tataagtgcc agaatcgttt
      301 ttttcatgat cgcttccgat gatcaaaggg agtactggaa tcccttgaca aacgtatgga
      361 ggacttttcc cttgagtttc tttccgaaaa agcatgagtt cacggatttt gaataaacgt
      421 ttttttgctc gaagacccat tcaaggtcca ggtccgcgag gataaagttc gcgtgctccc
      481 ccgctttgag ccggccgaaa tttgtttttt tcaggatctc gctcggcgcg atgctcatct
      541 tgcgcagcac gtcctcgatc ttccatttcc ggccgtgata gagttccgtc agcacaaccc
      601 cgaacgctga ttcgaggccg atcgttccga acggcgccac ttcgatatcc atgtcctttt
      661 cctcaaatgc gtgcggcgcg tgatcggtcg cgatcgcgtc gatcgtcccg tcctcaagac
      721 cgcggatcac ggcctgccgg tccttttcgg tcctgagcgg ggggttcatt ttgtaaaagg
      781 tgtcgtactt gacgaattcc tcgtcagtca gcgcgagatg gtgcggcgtt gcctcggccg
      841 tcacgggaag tccttcgtcc ttcgcgcgcc ggatcatcgc cactcccccg caggtggata
      901 cgtgctggac gtgaagatga catttgataa gccgggtcag ttcgatgtcg cgggcgatcg
      961 cgatgtcctc ggatgcgttc gggcggccgc ggagccccag ttttgttgag acaatgcctt
     1021 cgttcatcac accgttcagc gaaatcgtcg ggtcctcggc atgcgtcatg accgggagac
     1081 cgtaggtcgc ggcatattcg tacgccctgc gcgccacgcc ggaatccacg acccagttgc
     1141 cgtcatcggt gacggccacg gcgcctgata ctttcatctc cgccatctcc gcgacctttt
     1201 tgccctcgcg cccctgggtc
//
LOCUS       DAOOBX010000297         1212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_124534_length_1212_cov_3.583405, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000297 DAOOBX010000000
VERSION     DAOOBX010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124534_length_1212_cov_3.583405"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1212
                     /locus_tag="PLY30_04360"
     CDS             <1..>1212
                     /locus_tag="PLY30_04360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPW65374.1"
                     /translation="SVALLEKGDFASGTSSKSTKLIHGGLRYLENLEIDLVYESLKER
                     KLQLKAAPHLVRPLAFVIPVYEGDKRPLWMMRFGVFLYDLIAGPYKVGPHQSLSKEKV
                     LELEPGLEKEGLKGGVLYHDAQMDDARLCLENVLSADAAGAHVANYAKVTDYLRENGR
                     VTGVRAVDLLSRKTFGVCAKRIIVAGGPWSNELLRLDDPKAPEKVRTTKGIHIVYRDK
                     LSNHALFIPSKSDKRIFFVIPWLGHSLIGTTDTDYRGSADDVKVEPADVAYLMNETRR
                     VFPRSDLSEGKILSTFAGLRPLVKQGGSASKVSRKHLFYRSPSGVIFVVGGKYTTYRK
                     VAEDCLKRVFKKRDCPSWQKTGTDPFSAEAFQVCGSGSFVESDKDVSCRPGLDTDVVR
                     ELEGRYGSRSR"
BASE COUNT          246 a          348 c          378 g          240 t
ORIGIN      
        1 tcagcgttgc gcttctcgaa aagggtgatt tcgcgtccgg tacgtccagc aaatccacga
       61 agcttatcca cggcggcctc cgctacctcg aaaacctcga gatcgatctc gtctatgaat
      121 ccctgaaaga gcgcaagctc cagctcaagg ccgcgccgca cctcgtgagg ccgctcgcat
      181 tcgtgatccc ggtctacgag ggggacaagc ggccgctttg gatgatgcgc tttggcgtgt
      241 tcctctatga tctgatcgcg ggcccgtaca aagtgggccc ccaccaaagc ctttcaaaag
      301 agaaggtcct cgaactggaa cccggtcttg aaaaggaagg tctcaagggc ggcgtccttt
      361 accatgacgc gcagatggac gatgcgcggc tttgcctcga gaacgttctt tcggccgacg
      421 ccgccggcgc ccacgtcgcg aattacgcga aagtgacgga ttacctccgg gagaacgggc
      481 gcgtcacggg tgtgagggcg gtcgatctcc tctcacgaaa gacgttcggg gtgtgcgcga
      541 aacggatcat cgtcgcgggc ggcccctggt cgaacgaact cctgcgcctc gatgatccga
      601 aggcgccgga aaaggtccgg accacgaaag gcattcacat tgtttatcgg gacaagcttt
      661 cgaaccacgc gcttttcatc ccctccaaaa gcgacaaacg catcttcttc gtgattccgt
      721 ggcttggcca ctcgctgatc ggcacgacgg acacggacta ccgggggagc gccgacgacg
      781 tgaaagtcga acccgcggac gtggcgtatc tgatgaacga aacaaggcgc gtgtttccgc
      841 ggagtgatct ttcggaaggg aaaatcctct cgacgttcgc gggcctgcgg ccgctcgtga
      901 aacagggcgg ttcggcaagc aaggtttcgc gcaagcacct cttttaccgc tcgccgtcgg
      961 gtgtcatttt tgtcgtgggc gggaagtaca cgacttaccg gaaggtggcg gaggattgcc
     1021 tgaaacgcgt ctttaaaaaa cgggactgcc cctcttggca gaaaacggga acggatcctt
     1081 tttcggcgga ggcattccag gtgtgcggga gcggttcgtt cgtcgaatcg gacaaggacg
     1141 tctcctgccg gcctggtctt gacacggatg tcgttcggga actggagggg cgctatggtt
     1201 cccggagccg gg
//
LOCUS       DAOOBX010000298         1203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_125821_length_1203_cov_3.313589, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000298 DAOOBX010000000
VERSION     DAOOBX010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125821_length_1203_cov_3.313589"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLY30_04365"
     CDS             complement(<1..536)
                     /locus_tag="PLY30_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW65375.1"
                     /translation="MEGKNLYRRLRALEENRGTRAVWEGRKLLLFCSNDYLGLSRHPR
                     VVCAAKKALDRYGVGAGAARLISGSTDVHEALEKKIARFKNKENALVFGSGFLANLGI
                     LTAFAEEGDTIIMDKLCHASLVDGARLSKAELRVFPHRNYAKCENLLKKCPGRRKILV
                     TESVFSMDGDLADLGQIIR"
     gene            complement(602..>1203)
                     /locus_tag="PLY30_04370"
     CDS             complement(602..>1203)
                     /locus_tag="PLY30_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HPW65376.1"
                     /translation="ALAKRDIRPFDKRRDGFLVGEGAAVLFLETLESAKARGIRIYGE
                     VLDAACAQDSKHAILFDHGDHALSRALKTLLARSGISCAEIDYVHLHGTGTKPGDVYE
                     TAELKEAFGEAAYGMAMSSTKAATGHMLGATGAVEIAAMLVAMRDGFVPPTLNLEEKD
                     EACDLDYTPLRARKKKIAMALKVSMGFGGQAMALLLRKV"
BASE COUNT          250 a          377 c          318 g          258 t
ORIGIN      
        1 cggattatct gcccgaggtc cgcaaggtcg ccgtccatgc tgaaaacact ctccgtcacg
       61 agaatcttcc tgcggccagg gcatttcttc agaagattct cgcatttcgc gtagttcctg
      121 tgggggaaaa cccggagctc cgctttcgaa agacgggcgc cgtccacaag cgaggcatga
      181 cagagtttgt ccataatgat cgtgtcgcct tcttccgcga aggcggtcag gataccgaga
      241 ttcgcgagga aaccggaacc aaagacaagc gcattttcct tgttcttgaa ccgggcgatt
      301 tttttttcga gcgcttcgtg gacatccgtg ctccccgaga tcaggcgcgc ggcgccggca
      361 ccaaccccat agcggtcgag ggctttcttc gcggcgcaaa ccacgcgggg atgacgactg
      421 agtccgagat aatcattcga gcagaaaaga aggagctttc ttccctccca aacagcccgg
      481 gtcccgcggt tctcttcaag cgcgcgaagt cggcgataga ggttttttcc ttccaaggcc
      541 gtcaatccgg cccggacctc ctgatatatc gaaacattct ccaagacaga atcctcccgc
      601 attaaacctt tcgcaacaaa agcgccattg cctggccgcc gaagcccatg gaaaccttga
      661 gggccatcgc gatctttttc ttgcgtgccc gaagcggagt gtaatccagg tcgcaggcct
      721 cgtctttttc ttccagattc agggtcggcg gcacgaagcc atcccgcatc gccacgagca
      781 tcgccgcgat ctccaccgcc cccgttgcgc cgagcatatg tcccgtcgcg gccttggtcg
      841 agctcatggc cataccatag gcggcttccc cgaaagcctc tttgagctca gctgtttcat
      901 aaacatcgcc gggtttcgtc cccgtcccgt ggagatggac ataatcgatc tcggcacaag
      961 aaatgccgga gcgcgccaac aacgtcttca aggcccttga aagcgcgtgg tcgccgtgat
     1021 cgaacaggat ggcgtgtttt gaatcctggg cgcatgccgc atcgagaacc tctccgtaga
     1081 tccggatgcc gcgagccttc gcgctctcaa gcgtttctag aaaaaggacc gccgcgcctt
     1141 cgccgacaag aaacccgtcc cttcttttat cgaaaggacg tatgtcccgt ttcgcgagcg
     1201 cct
//
LOCUS       DAOOBX010000299         1200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_126326_length_1200_cov_2.103057, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000299 DAOOBX010000000
VERSION     DAOOBX010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126326_length_1200_cov_2.103057"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLY30_04375"
     CDS             complement(<1..204)
                     /locus_tag="PLY30_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65377.1"
                     /translation="MKLSASDALVSALSFAGSCALLWLFALDIGTVSFRANEKPLGTV
                     VFKKMTATRRPSDALGWERMRNDS"
     gene            complement(188..>1200)
                     /locus_tag="PLY30_04380"
     CDS             complement(188..>1200)
                     /locus_tag="PLY30_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPW65378.1"
                     /translation="RAATDVLRRNFLLSGVVLLISVLAVWFFSKSISRPVLSLVRAAH
                     RIEEGDFELDILPETRDELGLLTESFVEMGKGLAERERVKETFGKFVNKEIAEQALKG
                     ELKLGGQRKTATIFFSDIRSFTAISERLSPEAVVEFLNEYMTRMVACIEKTGGVVDKF
                     IGDAIMGVWGAPVSAGTPRDDALAAVRAMLMMRDELLEFNKGRGTPEKPFIHNGAGVN
                     TGPVIAGQIGSLSRMEYTVIGDAVNLASRIEALNKPFGTDVLISEYTWDLVKDAVIAE
                     PMPAITVKGKTAPLTIYAVVKMKGDEGPGSLSELREKLGIADPGKKIEVDKEEVKYEI
                     VG"
BASE COUNT          236 a          387 c          343 g          234 t
ORIGIN      
        1 gctgtcgttc cgcatccgct cccagccgag ggcgtccgag ggccggcgcg tagccgtcat
       61 cttcttgaag acgaccgtgc ccagcggctt ttcgttcgcg cggaaggaga cggtgccgat
      121 atcgagggcg aacagccaca gaagggcgca ggaaccggcg aacgagagcg ccgaaaccag
      181 ggcgtcgcta gccgacaatt tcatatttta cttcttcctt gtcaacttct atctttttcc
      241 cgggatcggc gattccgagc ttctctctca attcggaaag agacccgggt ccttcatccc
      301 ccttcatctt caccacggca tatatcgtaa gcggcgccgt tttgcccttt accgttatcg
      361 ccggcatggg ctccgcgatc accgcgtcct tgaccagatc ccaggtgtat tcggaaatga
      421 gaacatcggt tccgaagggc ttgttgagcg cctcgatgcg gctcgcgaga ttcaccgcgt
      481 cgccgattac cgtatactcc atgcgcgaaa gcgagccgat ctgcccggcg atcaccggac
      541 cggtgttcac gcccgcgccg ttatgaataa agggcttctc gggagtgccg cggcccttgt
      601 tgaactcgag cagctcgtcg cgcatcatga gcatcgcgcg aaccgcggcg agcgcgtcgt
      661 cccgcggagt cccggcggaa accggcgcgc cccacacgcc catgatcgcg tccccgatga
      721 atttgtctac gacgccgccg gtcttctcga tgcaggcgac catgcgcgtc atgtactcgt
      781 tcaaaaattc gacgacagcc tcaggagaga ggcgctcgga aatcgcggtg aacgagcgta
      841 tgtcggaaaa aaagatcgtc gcggttttgc gctgtccgcc gagcttgagt tcgcccttga
      901 gcgcctgctc ggcgatctcc ttgttgacga acttgccgaa ggtctccttc acccgctcgc
      961 gctcggcgag ccccttgccc atttccacga acgactcggt caagagtccg agctcgtcgc
     1021 gcgtttccgg cagaatgtcg agctcgaagt ccccctcctc tatccggtgc gccgcgcgca
     1081 caagggagag aaccggccgc gaaatcgact tggaaaaaaa ccacactgcc aaaaccgaga
     1141 taaggagaac cacgccggaa agaagaaagt tgcgcctgag cacgtccgtc gccgcgcggt
//
LOCUS       DAOOBX010000300         1189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_127926_length_1189_cov_3.531746, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000300 DAOOBX010000000
VERSION     DAOOBX010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127926_length_1189_cov_3.531746"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1019
                     /locus_tag="PLY30_04385"
     CDS             183..1019
                     /locus_tag="PLY30_04385"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPW65379.1"
                     /translation="MKKVGIVINQKKQGAVELAAKIRAWLGKRDLAVVDSMAQPVEQF
                     VEDADFLISLGGDGTILSIVGHLRNRSVPVLGVNLGSLGFLTEVKQSEVFEELTAYFQ
                     QPTIEDRLMLSCAAWSKTRQTERRFLALNDIVISREGLARILRLDVFVSGEKLTSFRG
                     DGVIIATPTGSTAYSLSANGAVVHPKLDVIVITPICPHALSLRSIVIGADEKINVQVR
                     TDDPAEKALLTADGQEKIEIDDSYTVEVGRSNTPFKLIKSTKRSYLETLRENFKLPDS
                     EK"
     gene            complement(994..>1189)
                     /locus_tag="PLY30_04390"
     CDS             complement(994..>1189)
                     /locus_tag="PLY30_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65380.1"
                     /translation="GTREIDLEAFMLHGFAELFADRLFVIHDQQSDDNPPRSGQEMPL
                     TAYPVVKHHPTEIISRNPAA"
BASE COUNT          263 a          330 c          351 g          245 t
ORIGIN      
        1 tcgaacacgg ttcccgtgaa gcccagctcg aactctacgg cctgtccccc gaaaaggtcg
       61 ccgccgccgt cctcaaagaa gtccaggttt tgccgcagcg cggttcctga ttccaaaaca
      121 gctcgtttgc gtccttgatg cccgggaccg tcccgcttaa aatacccgcg gagggcacat
      181 ccatgaaaaa agtcgggatc gtcattaatc agaaaaaaca gggtgccgtg gagctcgcgg
      241 cgaagattcg tgcgtggctc gggaaacggg acctcgcggt cgtggattcg atggcccagc
      301 ccgtcgaaca gttcgtagaa gatgcggact tcctgatatc gctcggcggg gacgggacca
      361 tcctttccat cgtcggccat ctccgtaacc ggtccgtccc ggtgctcggc gtaaacctcg
      421 gaagtctcgg attcctcacg gaggtgaaac aatccgaggt ctttgaagag ctcacggcct
      481 atttccagca gcccacgatc gaggaccggc tgatgctttc ctgcgccgcg tggagcaaaa
      541 cgcgtcaaac ggagcggcga tttctggccc tgaacgacat cgtgatcagc cgcgaagggc
      601 tggcccgcat cctgcgcctg gacgtgtttg tgtcagggga gaagctcacg agtttccgcg
      661 gggacggagt gattatcgcg acgccgacgg ggtccaccgc ctattcgctt tcagcgaacg
      721 gcgcggtcgt ccatccgaaa ctcgacgtca tcgtgattac gccgatctgc ccgcacgcgt
      781 tgagcctgcg gtccattgtg atcggtgcgg acgagaagat taacgtgcag gttcgtaccg
      841 atgatcccgc cgaaaaggcg cttttgacag ccgacggtca agaaaagatc gaaatcgacg
      901 atagctatac cgttgaggtg gggcgttcga ataccccatt caaattgatc aaaagcacga
      961 aacggagtta tctggaaacg ctccgggaaa atttcaagct gccggattcc gagaaataat
     1021 ttccgtcgga tggtgtttca caaccggata tgccgtcaaa ggcatttcct gtcctgagcg
     1081 gggtgggtta tcgtcagact gttgatcgtg gatgacgaaa agtcgatctg cgaagagttc
     1141 cgcgaaacca tggagcatga aggcttcgag gtcgatttcg cgagttccg
//
LOCUS       DAOOBX010000301         1182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_128981_length_1182_cov_2.504880, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000301 DAOOBX010000000
VERSION     DAOOBX010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128981_length_1182_cov_2.504880"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..425)
                     /locus_tag="PLY30_04395"
     CDS             complement(12..425)
                     /locus_tag="PLY30_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW65381.1"
                     /translation="MELETIKHIDPAFVDARGEIYNLFEGDIGHVAYITSKKGSVRAN
                     HYHKKDIQYMYLISGAYETHCFPVGDPSRKQILNVRPGDIVKTPPMVAHAQKFTEDSV
                     FLSLTTRMREEGKYEEDTIAISVIEGYLNKELKTR"
     gene            complement(438..>1182)
                     /locus_tag="PLY30_04400"
     CDS             complement(438..>1182)
                     /locus_tag="PLY30_04400"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPW65382.1"
                     /translation="HAAGIASPVYYTKYPVETMDVGTIGTRNMLELAREKKVKSFLFT
                     SSSEVYGDPDSRHVPTSEEYRGNVAVIGPRACYDESKRFGETMCSAFWNVYETPVKMV
                     RPFNVYGPGIRPDDYRVLPNFIEHALRKEPLPIHGDGRNTRSFCYINDGVESIFRVLF
                     SDANGEAFNIGNPEPEISIEELARYVQKAMPYPVEVVKIDPPHAVYSKSDPKRRCPDI
                     TKVSTVTGYKPRYSLEEGLKRTIAWFQEF"
BASE COUNT          228 a          339 c          341 g          274 t
ORIGIN      
        1 ctcccggaat cttatcgggt cttcagttct ttattgagat agccctcgat cacggagatc
       61 gcgatcgtat cttcctcgta cttcccctct tcccgcatgc gcgtcgtgag ggagaggaaa
      121 acggaatcct cggtaaactt ctgggcgtgc gcgaccatcg gcggcgtctt cacgatatcg
      181 ccgggccgca cgttcaggat ctgcttccgg gaagggtctc cgacgggaaa gcagtgagtc
      241 tcataggccc cgctgatgag gtacatgtac tggatgtctt ttttatggta atggttcgcg
      301 cggaccgaac ctttcttcga ggtaatgtag gcgacgtgac caatatcgcc ttcaaaaagg
      361 ttgtaaatct cgccgcgggc gtcaacgaac gcggggtcaa tgtgcttgat cgtttccaat
      421 tccatttcga tctcctttta gaattcctgg aaccaggcaa tggtgcgttt cagcccttcc
      481 tcgagactat aacgcggttt ataaccggtg acggtggaaa ccttcgtgat gtccgggcag
      541 cgccgtttgg gatcgctctt cgaatagacc gcgtgcggcg ggtcgatctt gacgacctcg
      601 accggatacg gcatcgcctt ctgcacatag cgcgcgagct cctcgatcga aatctcgggc
      661 tcgggattgc cgatgttgaa ggcctcgccg ttcgcgtcgc tgaaaaggac gcgaaaaatc
      721 gattcgacgc cgtcattgat gtagcagaaa gaccgcgtgt tgcgcccgtc accgtggatc
      781 ggaagcggtt ccttccgcag cgcgtgctcg atgaagttcg gaaggacgcg gtaatcgtcg
      841 gggcggatgc cggggccgta cacgttgaac ggacgcacca tcttgaccgg cgtttcgtag
      901 acgttccaaa acgcactgca catcgtctcg ccgaaacgct tcgactcatc gtagcaggcc
      961 cgcggaccga tcacggccac gttcccccgg tactcctcgg acgtcgggac atgccggctg
     1021 tcgggatccc cgtagacctc gcttgagctc gtgaaaagaa aactcttcac tttcttttcg
     1081 cgtgcgagtt ccagcatgtt gcgggtgccg atcgtgccga cgtccatcgt ctcgaccgga
     1141 tatttcgtgt aatagaccgg cgaagcgatc cccgccgcgt gg
//
LOCUS       DAOOBX010000302         1171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_130772_length_1171_cov_1.674731, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000302 DAOOBX010000000
VERSION     DAOOBX010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130772_length_1171_cov_1.674731"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLY30_04405"
     CDS             <1..476
                     /locus_tag="PLY30_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65383.1"
                     /translation="DSRAMLAAETEAMKKLLENPVSEALFDRMIEAGDILALAQNRTI
                     DPARLPDRLVFSKGVERFEVRNEDPSLPDIEVLLQGTPRFKRFAQKYPFAIMKSGYAN
                     ERFVVLPESPAALVLYRLIALDAGLYRAARNPEFVKLLEGLVAEILSSGIVERAA"
     gene            complement(510..956)
                     /locus_tag="PLY30_04410"
     CDS             complement(510..956)
                     /locus_tag="PLY30_04410"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPW65384.1"
                     /translation="MHLLTKSILINAPIEAVWGLLADVKRMPEWIDGVKESERTGAVT
                     EGKGLRWRESCEFGRQHLETENEITVWEPMERAVIESKLPMNGIMKRTHAFGKKEEAV
                     EVSVRVEWDLGVAGMLLGEQKVRDILDQSFSATLLNWKERAEKITG"
     gene            complement(1041..>1171)
                     /locus_tag="PLY30_04415"
     CDS             complement(1041..>1171)
                     /locus_tag="PLY30_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPW65385.1"
                     /translation="RNVGWRIDYHFVTEALAPRVKSAVILKDVVGSDHCPVGIELK"
BASE COUNT          243 a          354 c          302 g          272 t
ORIGIN      
        1 cggattccag ggccatgctc gccgcggaaa cggaagcgat gaaaaaactt ttagaaaatc
       61 ccgtttcgga agcgttgttt gaccggatga ttgaagccgg ggacatcctc gccctcgctc
      121 aaaaccggac tatcgatccc gctcggctcc ccgaccggct tgttttttcg aagggcgtcg
      181 aacgttttga ggtccgcaac gaggatcctt cgcttccgga tatcgaggtc ctgcttcagg
      241 gaactccccg ttttaaacgc ttcgcccaga aatatccgtt tgcgatcatg aaatccggct
      301 acgcgaacga aaggttcgtc gttctcccgg aatcccctgc cgcgcttgtt ctttaccggc
      361 ttatcgcgct ggacgcgggg ctctacaggg ccgcaagaaa tccggagttc gtgaaattgc
      421 tggaggggct tgtagccgag atcctgtcga gcgggatcgt ggaacgggcc gcttaaaacc
      481 gccgcgcaag cggcgggcgg agtcaaacgt catccggtga ttttctcggc tctttccttc
      541 cagttcaaaa gcgtcgccga aaaactctgg tccagaatat cccggacttt ctgctccccg
      601 agaagcatcc ccgcgacgcc gaggtcccac tcgactctga ctgaaacttc aacggcttcc
      661 tcttttttcc cgaaagcatg agtccgtttc atgataccgt tcatggggag tttgctttcg
      721 atcacggcgc gctccatcgg ttcccacacg gtgatctcat tctcggtttc gagatgctgg
      781 cggccaaact cgcaggactc gcgccatcgc aaccccttcc cttccgtgac agctccggtc
      841 cgctcgcttt ccttgacacc gtcgatccat tccggcatac gcttcacgtc ggccaggagt
      901 ccccatacgg cttcaatcgg ggcattgatc aaaatagatt tagtaagtag atgcataaac
      961 accgcaagac aacggattgg ggacggagga agtcgataga ctgaatcttg cgctacccgc
     1021 tatccgccgc ccggttttgt tcatttgagt tctatcccca ccgggcaatg atccgatccg
     1081 acaacatcct tcagaatcac ggcggatttg acgcgcggcg cgagtgcttc tgtcacaaaa
     1141 tgatagtcga tccgccagcc gacattccgc t
//
LOCUS       DAOOBX010000303         1155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_133251_length_1155_cov_2.870909, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000303 DAOOBX010000000
VERSION     DAOOBX010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133251_length_1155_cov_2.870909"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1155
                     /gene="cmk"
                     /locus_tag="PLY30_04420"
     CDS             <1..>1155
                     /gene="cmk"
                     /locus_tag="PLY30_04420"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPW65386.1"
                     /translation="SPAPALVREALDRVLRPEARDANGALSIERNPLTRHVVTIDGPS
                     GTGKGTAAKNLAKRLGYMHVDLGMIYRALTRKALDRRLDANDESVVNRLVSDTAIELR
                     KGEDGTRVFLDGKDVTNAIRSPEVDRSVADIARHFSVARFVFQTARRIGESEDIVIEG
                     RNAGTDIFPDANAGKFYLITDLAEKARRRWEDYKKTQPGIALEEVERDLALRDEKDRT
                     RKIAPLRQAPDAIVINNTHLQAIEVTDRMESLVRKELSSSRVESVLQKIADEIKQGGS
                     TQAKAVDLVWKGVRGEIVAANVLIADGKVLRRKWDPALKEILESLLTRNVKFEVLRGN
                     SPVSGHYAAVILREEGAEFVKPPRESGTPGAEQGTHKRSEARTIDLNLSL"
BASE COUNT          249 a          317 c          379 g          210 t
ORIGIN      
        1 ggtccccggc ccccgcgctc gtgcgggaag ctctcgaccg cgtgcttcgg cccgaggcgc
       61 gcgacgcgaa cggtgccctg tcgatcgaac ggaatccgct tacccgtcat gtcgtgacaa
      121 tcgacggacc ttcgggaacg ggcaaaggga ccgcggcgaa gaacctcgcg aagcgcctcg
      181 gctacatgca tgtggatctc ggaatgatct accgcgcgct gacccgtaag gcgcttgatc
      241 ggcggctgga cgcgaatgac gaaagcgttg tgaaccggct tgtgtctgat acggccattg
      301 agctcaggaa aggggaggat gggacgcggg tcttcctcga cgggaaggat gtgacgaacg
      361 ctatccggtc gccggaagtg gacaggagtg tcgctgatat tgcccggcat ttctccgtgg
      421 cgcgttttgt tttccagacc gcgcggcgca tcggcgagtc ggaagatatc gtgatcgaag
      481 ggcgcaacgc gggaacggat atttttccgg atgccaacgc gggcaagttc tatctgatta
      541 cggaccttgc cgagaaggcc cgccgccgct gggaagatta caagaagacg cagcccggta
      601 ttgcgctgga agaagtggaa cgtgatcttg ccttgcgcga tgagaaggac aggacccgga
      661 agatcgctcc gcttcgccag gcgccggacg ccatcgtaat caacaacacc catcttcagg
      721 ccatcgaagt taccgacagg atggaatccc tggtccggaa ggagctttcg tcttcacgcg
      781 tcgagagcgt gcttcagaag atcgctgatg agatcaaaca aggcggcagt acgcaggcga
      841 aggccgtgga ccttgtctgg aagggcgtgc gcggagagat cgtggccgct aatgtcctca
      901 tcgcggacgg gaaggtcctg cggcgcaaat gggatccggc cttgaaagag atactggaaa
      961 gtcttttgac tcgcaatgtg aagttcgaag tgctcagggg aaattccccg gtttcaggcc
     1021 attacgccgc cgtgatcctc cgggaggaag gggctgagtt cgtgaagcct ccccgagaat
     1081 ccggaacgcc gggagcggaa cagggcactc acaagcggtc cgaagcgcgg accatagatt
     1141 taaatctatc cctcc
//
LOCUS       DAOOBX010000304         1153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_133575_length_1153_cov_3.250455, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000304 DAOOBX010000000
VERSION     DAOOBX010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133575_length_1153_cov_3.250455"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLY30_04425"
     CDS             <1..640
                     /locus_tag="PLY30_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPW65387.1"
                     /translation="QYYKIQSESRASCHTAAMKALQEEGSDLLSVSLAIGGKIARHTL
                     SVKFGGAGAAARVLGLSLGADRQVLDHVTGIDHASPGCKSDQLFKSILAGAARGVFTG
                     KVLVRRGAQKTDAHQSSKNLLLSETAKADARPQLEILADDVKCAHGAAVGQLEEDPVF
                     YLRSRGIGEKEARRILAAGFANEVTDAIPLEGLRELAGVRTDEKLGEMEAEA"
     gene            637..>1153
                     /locus_tag="PLY30_04430"
     CDS             637..>1153
                     /locus_tag="PLY30_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPW65388.1"
                     /translation="MNGLDVAKVREDFPVLHERVYGKPLIYLDNAATTQKPKIVIETI
                     KQFYSRENANIHRGIFCLSERATRRYENARLKVKEFLGAASEKEIVFVRGATEAINLV
                     AQTWGRAFLGKGDEVLITHMEHHSNIVPWQILREEKGIKLKVAPVDDAGELIMEEFRK
                     LLTDKVKLVSVV"
BASE COUNT          253 a          315 c          387 g          198 t
ORIGIN      
        1 gcaatattac aagatccaga gtgagagccg cgcctcctgc cacacggccg cgatgaaagc
       61 gctccaggag gaaggaagcg atcttctctc cgtgtcgctg gccattggcg gaaagattgc
      121 gcggcacacg ctgtcggtga aattcggtgg cgcgggagcc gcggcgaggg tcttgggtct
      181 ttccctcggg gccgaccgcc aggttctcga tcatgtgacc gggatcgacc atgcgagccc
      241 ggggtgcaaa agtgatcagc ttttcaaaag catcctggcc ggcgcggcgc gcggcgtctt
      301 caccggtaag gtgctcgtgc ggcggggcgc gcagaaaacg gacgcccatc agtccagcaa
      361 aaacctgctc ctgtcggaga cggcgaaagc cgacgcgcgg ccacagcttg agatcctggc
      421 ggacgacgtt aaatgcgccc acggcgcggc ggtcggtcaa ctggaagagg atccggtctt
      481 ttacctgagg tcccgcggga tcggcgaaaa agaagcgagg cgtatccttg ccgcgggttt
      541 cgcgaacgag gtgacggatg cgatcccgct cgaaggcctg cgggagcttg ccggagtccg
      601 gacggatgaa aaactcggtg aaatggaggc ggaagcatga acggtctgga tgtggcgaag
      661 gtccgggagg attttccggt gctgcatgaa cgagtctacg gaaaacccct gatctatctc
      721 gataacgccg cgacgaccca aaagccgaag atcgtgatcg aaacgatcaa acagttctac
      781 tccagggaga acgccaatat ccaccgcggg atcttttgcc tgagcgagcg ggccacccgg
      841 cgctatgaga acgcccggct caaggtgaag gaatttctcg gtgccgcgtc cgaaaaagaa
      901 atcgtgttcg tgcgcggggc cacggaggcg atcaacctcg tcgcgcagac ctggggccgc
      961 gcctttctcg ggaaagggga tgaggtgctg attacgcaca tggagcacca ctcaaacatc
     1021 gtgccgtggc agatacttcg cgaggagaag gggatcaagc tcaaggtcgc gcccgtggat
     1081 gatgccggcg agctgattat ggaagagttc cggaagctcc tgacggacaa ggtgaaactt
     1141 gtttcggtgg tcc
//
LOCUS       DAOOBX010000305         1135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_136744_length_1135_cov_1.962037, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000305 DAOOBX010000000
VERSION     DAOOBX010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136744_length_1135_cov_1.962037"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..975)
                     /gene="thrC"
                     /locus_tag="PLY30_04435"
     CDS             complement(22..975)
                     /gene="thrC"
                     /locus_tag="PLY30_04435"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPW65389.1"
                     /translation="MKEYAVKRTGIVERYQEYLPVTDKTPIISLNEGNTPLILAEALP
                     KELGLKGVRIYYKFEGLNPTGSFKDRGMTMAISKAVEAGAKAVMCASTGNTSASAAAY
                     ACRCGLKCFVLIPEGKIAKGKLLQAFRYGAKTLMIKGNFDQALELVKEMTKDGKITLV
                     NSINPYRIEGQKTASFEIVDDLGEAPEYHAIPVGNAGNITAYWKGYKEYQKAGKSEVL
                     PKIEKTGLWVTDIEVLRLHYAETIAQWRARFAGNRDIIASLTDDRFCRMFEFYLAGAE
                     LSFRHMGHMVWQVQMTRRIDALPLTRDYMLEAERVATRAQP"
BASE COUNT          199 a          367 c          305 g          264 t
ORIGIN      
        1 ggggaaaacg gggatgaccg gtcacggctg cgcgcgtgta gccacccgtt ccgcctccag
       61 catgtagtcg cgcgtcagcg gcaaggcatc gatccgccgc gtcatctgca cctgccagac
      121 catgtgcccc atgtgacgga aggagagttc cgcccccgcc aggtagaatt cgaacatgcg
      181 gcagaaacgg tcatcggtca ggctggcaat gatgtcgcgg ttgccggcga aacgcgcccg
      241 ccactgcgcg atcgtctcgg cataatgcag gcgcagcact tcgatgtccg tcacccacag
      301 gccggtcttc tcgatcttcg gcaggacttc ggacttcccc gccttttgat attccttgta
      361 gcctttccag tacgccgtga tattgccggc gtttccgacc gggatcgcgt ggtattccgg
      421 cgcctcaccg agatcgtcca cgatctcgaa cgaggccgtt ttctgtcctt cgatccggta
      481 cgggttgatt gaattgacga gcgtgatctt gccatccttc gtcatttcct tcacaagctc
      541 aagcgcctgg tcaaagttcc ccttgatcat cagcgtcttc gcaccataac ggaacgcctg
      601 gagcagtttc cctttcgcga tcttcccttc cgggatcagc acaaaacatt tcagcccgca
      661 gcggcacgcg tacgcggcgg cggacgctga cgtattaccc gtcgaggcgc acatcacggc
      721 cttcgccccc gcctccacgg ccttcgaaat cgccatcgtc atcccgcggt ccttgaagga
      781 gccggtcggg ttcagacctt caaacttgta gtagattcgc acgcccttga gcccgagttc
      841 tttcgggagg gcctccgcta ggatcagcgg cgtgttcccc tcgttcagcg agatgatcgg
      901 cgtcttgtcc gtgaccggga gatactcctg gtagcgttcg acgatacctg ttctcttaac
      961 cgcgtattcc ttcatcttta acctctgttc agcggacaga aaatctttcc ttcatgctat
     1021 tcaggtaccc gcttcttcca tacggatgac ttgagccttg cctttcacga acgactgccg
     1081 gtcaatcacc cggatcgcgg cgcggatgtc cttttcgggg gcctcgtgcg tcagg
//
LOCUS       DAOOBX010000306         1134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_136851_length_1134_cov_2.972196, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000306 DAOOBX010000000
VERSION     DAOOBX010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136851_length_1134_cov_2.972196"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLY30_04440"
     CDS             <1..432
                     /locus_tag="PLY30_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW65390.1"
                     /translation="EEIHHAKEEGIVFRLLTNPVKIVGKDGWVTGMECVEMELGEPDE
                     SGRRRPVVKKESNFVFPVETVIVAIGGTPNPLIRQTTPGLEVQKWGGIVVDEATMKTS
                     KENVYAGGDIVTGAATVILAMGAGKKAAKAIHEALQNKIPA"
     gene            569..1006
                     /locus_tag="PLY30_04445"
     CDS             569..1006
                     /locus_tag="PLY30_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPW65391.1"
                     /translation="MKLITRNTDYALRALCYIAEQEKDSVSVPEMVAALHIPRPFLRK
                     LLQTLSAEGILQSFKGQGGGFTLARDPEQIRLTELIRIFQGTVELNECIFKKKVCPNR
                     ATCRLKREIDAIEKDVLRRLSAINIASLTAHRRIRPKEGVRAR"
BASE COUNT          267 a          292 c          359 g          216 t
ORIGIN      
        1 gaggagattc accacgcgaa ggaagagggg attgttttca gactgcttac gaaccccgtg
       61 aaaatcgtcg gcaaggacgg ttgggtgacg gggatggaat gcgtggaaat ggagctcgga
      121 gagcccgatg aatcggggcg ccggcgtccg gtcgtgaaaa aagaaagcaa ctttgttttt
      181 ccggtcgaga ccgtgatcgt ggcgatcggc ggaacgccga accctctcat ccgtcagacc
      241 accccgggac ttgaggtcca gaaatggggc ggcatcgtgg tggacgaagc gacgatgaag
      301 acctcaaagg agaatgtcta cgcgggcggg gatatcgtga ccggcgccgc gaccgtgatc
      361 ctcgccatgg gtgcggggaa aaaagccgcc aaggccattc acgaggccct tcaaaataaa
      421 atcccggcct gatccccgaa agggagggac gggattttta ttgttgacga atgatactaa
      481 tgcggtatca ttaagtccgc gagcgtagcg cgcgggtttg ctcgccccat cccgacgtca
      541 gttcttgaag aaggggagtc ttgacggagt gaagctgatc acacggaaca cggattatgc
      601 gttgcgcgcg ctctgctata tcgcggagca ggagaaggat tcggtctccg tccccgaaat
      661 ggtggcggct cttcatatcc cgaggccgtt cctccggaag ctgctccaga ccctcagcgc
      721 ggaaggcatc ctccagtcct ttaaaggcca gggaggaggt ttcacgttgg ccagggatcc
      781 ggaacagatc aggctgaccg aactcatacg catcttccag gggactgtcg agctcaatga
      841 atgtatcttc aaaaagaagg tctgcccgaa tcgcgccacc tgcagattga aacgcgagat
      901 cgatgcgatc gaaaaagacg ttctgcggcg cttgtctgcc atcaatatcg cttctttgac
      961 tgctcatcgg cgcatcaggc cgaaggaggg ggttcgtgcg cgttgaaggt gcggccgcag
     1021 aggcccgccc ggagggggac gacaggagcc cggtcccgaa acgggaatcc gtttgaataa
     1081 aagggggttc cgtgttccaa taacaaaagt tcgacatctc agattcccga tgcg
//
LOCUS       DAOOBX010000307         1110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_141230_length_1110_cov_3.094787, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000307 DAOOBX010000000
VERSION     DAOOBX010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141230_length_1110_cov_3.094787"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /gene="tpx"
                     /locus_tag="PLY30_04450"
     CDS             complement(<1..444)
                     /gene="tpx"
                     /locus_tag="PLY30_04450"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPW65392.1"
                     /translation="MIQERKDAAAFQGNPLTLIGPELKTGDKAPEFTAVDNALKPVTL
                     ASSRGKTRLVSVVPSIDTPVCELQTKRFNQEAAKLPASVVVLTVSMDLPFAQGRWCGT
                     AGIDKVQMLSDHQQASFGAAYGVLIRELRLLARAIFVIGPDDRIKY"
     gene            complement(513..875)
                     /gene="queF"
                     /locus_tag="PLY30_04455"
     CDS             complement(513..875)
                     /gene="queF"
                     /locus_tag="PLY30_04455"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HPW65393.1"
                     /translation="MSTQPSKQLEIFKNPNPRRDYEIDLECPEFTCLCPKTGQPDFAI
                     VRIRYTPDEFCVELKSLKLYLWSYRNEGAFHEAVTNKILDDLVRAVKPRRMSVEADFY
                     VRGGIHTVVTATYSKKKK"
     gene            complement(884..>1110)
                     /locus_tag="PLY30_04460"
     CDS             complement(884..>1110)
                     /locus_tag="PLY30_04460"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HPW65394.1"
                     /translation="RSILFFGGRAPVSPSLPYEWYRTPNLRFLSIKDFRNFCSQKHYT
                     VLEVKYLGKKRVVKFFPNLFAANAIFVIAK"
BASE COUNT          247 a          312 c          307 g          244 t
ORIGIN      
        1 atatttgatc ctgtcatccg gcccgatcac aaagatcgcg cgcgcgagca gccgcaactc
       61 cctgatcaga acaccgtaag ccgcgccgaa cgaggcctgt tggtgatccg agagcatctg
      121 gaccttgtcg atgcccgcgg tcccgcacca gcgcccttgc gcgaacggca gatccatgct
      181 aaccgtcagg acgaccacac tcgccgggag tttcgcggcc tcctggttga accgctttgt
      241 ctgaagctca cagaccggcg tatcgatcga aggaaccacg ctcacaagcc gcgtttttcc
      301 cctggacgac gcgagcgtca cgggtttcag cgcgttatcc acggctgtga actccggcgc
      361 cttgtcaccc gtcttgagtt ccggaccgat cagcgtgagg gggttaccct gaaatgcagc
      421 agcgtctttt cgttcctgta tcatattcgc ctccgttcat tgtaaaaaac cgggacgccc
      481 atttttgaga acggaatcga gcgtcctcgt tgttattttt tctttttgga atacgtcgcg
      541 gtgacaaccg tgtgaatgcc gccacggacg tagaaatccg cctcaaccga catccggcgc
      601 ggcttcacgg ctcgcacgag atcatcgagg atcttgttcg tgaccgcctc gtgaaaagca
      661 ccttcgttac ggtaactcca gagataaagc ttcaggctct tgagctccac acagaattcg
      721 tcgggagtat agcgaatgcg gactatcgca aaatcaggct gtcccgtttt cggacagaga
      781 cacgtgaatt ccggacactc gaggtcgatc tcgtaatcac gacgggggtt cgggtttttg
      841 aagatctcaa gttgtttgga cggctgggtc gacatgggag cggtcacttc gcgataacaa
      901 aaatggcgtt ggcggcaaaa agattcggaa agaacttcac gaccctcttt ttcccgaggt
      961 atttcacctc gagaaccgtg taatgttttt gcgagcagaa gttcctgaaa tccttgatgg
     1021 acagaaagcg caggttcggg gtccggtacc actcgtaagg aagcgacgga ctgacggggg
     1081 cacggccgcc gaagaaaagg atcgaccggc
//
LOCUS       DAOOBX010000308         1105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_142241_length_1105_cov_1.978095, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000308 DAOOBX010000000
VERSION     DAOOBX010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142241_length_1105_cov_1.978095"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLY30_04465"
     CDS             complement(<1..845)
                     /locus_tag="PLY30_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPW65395.1"
                     /translation="MKPVHLPVNPKILVTRTDRIGDLVLTTPVFQALRERFPKAWIAA
                     LVFIEHRDIVKDNPYLDEVILYDKKGSEKGLWGQFCFSVRLRAKKFDLVIHCHGTNRM
                     HLAGWLAGVPVRLGYDRRAPWALTHAHAYDKKLGEKRETEYLFDLLAPLGIACPGKIE
                     ACFPVSGSAARSLENLLFHLKVPADKKLIVLSPSASDRTKMWPAAKFAELAARLQKEA
                     GAVLLAVGSLKDKPVVESLREKSAVPIVDLGGKLTLGMLGALLKRAALLVSNDSGPVH
                     IATAVG"
     gene            complement(842..>1105)
                     /locus_tag="PLY30_04470"
     CDS             complement(842..>1105)
                     /locus_tag="PLY30_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPW65396.1"
                     /translation="ISKAALVIANDSAIMHLGYETGTPTIGLFGPTDHEKYGHEGPGF
                     RIATGDAANCACQSHLLPYAERSCFHGLDSGAVLKLCRELLIP"
BASE COUNT          236 a          341 c          309 g          219 t
ORIGIN      
        1 ccgaccgccg tcgcgatatg gacgggcccg gaatcattgg aaacaagcag cgcggccctc
       61 ttcaggagcg ccccgagcat cccaagggtc agcttccccc ccagatccac gatgggcacc
      121 gcactcttct cccgaagact ttcaacgacc ggcttgtcct tgagcgatcc gaccgccaga
      181 agaacggccc ccgcttcttt ctgaagccgc gcggccagct ccgcaaattt cgcggcaggc
      241 cacatcttgg tcctgtcgct cgccgaggga ctgagaacta tcagtttttt gtccgcggga
      301 acttttaaat gaaacaggag gttctcaagg gagcgcgcgg cgcttccgga caccgggaaa
      361 caggcctcga tcttcccggg acaggcgatc ccgagcggcg cgagaaggtc gaagagatat
      421 tcggtctccc gcttctcgcc gagttttttg tcgtaggcat gcgcgtgcgt gagcgcccac
      481 ggagcgcggc gatcataccc gagacggacg ggaacgccgg caagccatcc cgcaagatgc
      541 atccggttcg tgccatggca gtggatcaca agatcgaact tcttcgcgcg gagccgcacg
      601 gaaaaacaga attgtcccca gagacctttt tcgcttccct ttttatcgta aaggatcacc
      661 tcatcaagat acggattgtc cttcacgatg tcccgatgct cgataaaaac aagcgcggcg
      721 atccacgcct tcgggaaccg ttcccggagc gcctgaaaga ccggcgtcgt gagcacaagg
      781 tccccgatcc ggtccgtccg cgtgacaagg atctttggat ttacaggaag atgaaccggt
      841 ttcatgggat caaaagctcc ctgcagagtt tcagaaccgc cccggaatcc aatccgtgga
      901 agcaactgcg ttcagcgtag ggcaggagat gtgactgaca ggcgcaattc gccgcgtctc
      961 ccgttgcgat cctgaaacca gggccttcgt gaccgtattt ttcatggtcc gtcgggccaa
     1021 aaagtcctat cgtcggcgtc ccggtttcgt aaccgagatg catgatcgcg ctgtcgttcg
     1081 cgatcacaag cgccgctttc gaaat
//
LOCUS       DAOOBX010000309         1102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_142668_length_1102_cov_3.727794, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000309 DAOOBX010000000
VERSION     DAOOBX010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142668_length_1102_cov_3.727794"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1102)
                     /locus_tag="PLY30_04475"
     CDS             complement(<1..>1102)
                     /locus_tag="PLY30_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPW65397.1"
                     /translation="YQCQGHFFTNLKSLRKDGYYSRDNWELSVRERRRGFKQMRAKPN
                     RLSELLDHLIREDRLPAIYFAFSRKRTVYLAWEIAHYDLLNKAERAKVTELFDELCKK
                     YDLAEERSAQDMKPLIEHGIAYHHAGMLPTLKEVVERLFTSRLIKLIFTTETFALGIN
                     MPARTVIFDELEKFYGTGFKALTTRDFYQMAGRAGRRGMDAKGFVYCRILPHDIPFPQ
                     VERIIFGHPEPVRSQFNAAYATVLNLYRDFGEDLLDVYPRSFHYFQSSRRMRDDGFDL
                     IRSKLNLLKELGYIRDGKLSEKGEFASSIFGYELLLTEMYQDKVFESFDEARLNVFLA
                     GLVFEPRKGLDEPPRLSHQNEQLLKVNQQYHR"
BASE COUNT          219 a          289 c          341 g          253 t
ORIGIN      
        1 ttccggtggt actgctggtt gaccttgagc agttgttcgt tttgatgcga aagcctgggc
       61 ggctcatcaa gccctttacg gggctcaaac acgaggcccg cgaggaatac gttgagccgc
      121 gcctcgtcga agctctcgaa gaccttgtcc tggtacattt ccgtgagcaa cagttcatag
      181 ccgaaaattg aggacgcgaa ttcccccttt tccgaaagtt tcccgtcgcg gatgtatccc
      241 agctctttta acagattcag cttgctgcgg atcaggtcga aaccgtcatc gcgcattcgc
      301 cgcgaggact ggaaataatg gaaggacctc ggataaacat caaggagatc ctccccgaaa
      361 tcacggtaga ggttgagaac ggtcgcgtaa gcggcgttga actggctgcg gacgggttcg
      421 ggatggccga aaatgatgcg ctcgacctgc gggaagggga tgtcatgcgg caggatgcgg
      481 caatagacga accctttcgc gtccatgccc cgtcgccccg cgcgacccgc catctggtag
      541 aaatcgcggg tcgtgagggc cttgaagccc gtcccgtaga atttttcaag ttcgtcgaag
      601 atgaccgttc gcgccggcat attgattccg agcgcgaagg tttcggttgt gaagatcagc
      661 ttgatcaggc ggctcgtgaa aaggcgttcg accacttcct tgagcgttgg aagcatgccc
      721 gcgtggtggt aggcgatgcc gtgttcgatc aggggtttca tgtcctgcgc ggagcgttct
      781 tccgcgaggt cgtatttttt gcagagttcg tcgaagagtt ccgtgacttt cgcgcgctcg
      841 gccttgttca gcaggtcgta gtgcgcgatc tcccaggcga ggtagaccgt gcgtttccgg
      901 ctgaaggcaa aataaatagc ggggaggcgg tcctcgcgga tcaggtgatc gagcagttca
      961 ctcaggcggt tcggtttcgc gcgcatttgt ttgaacccgc gccgccgctc acgcacggaa
     1021 agttcccagt tatcgcggga atagtagccg tccttgcgca gcgatttcag gttcgtgaag
     1081 aaatgcccct ggcattggta ga
//
LOCUS       DAOOBX010000310         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_143449_length_1098_cov_2.472675, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000310 DAOOBX010000000
VERSION     DAOOBX010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143449_length_1098_cov_2.472675"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="PLY30_04480"
     CDS             <1..166
                     /locus_tag="PLY30_04480"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPW65398.1"
                     /translation="KEKRTGIDAINGMVVRLGKKHKVPTPVNAFLSPSIRKAERCSWT
                     TTGTSRGKTA"
     gene            163..543
                     /locus_tag="PLY30_04485"
     CDS             163..543
                     /locus_tag="PLY30_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPW65399.1"
                     /translation="MRQKTVICAAAGVLLAVFLNGCASSARFVAVPSGPLSQKPYVAV
                     YGKEGCPACADLKKDLARKGTDYLDKDISDPGIKRELVSRMETAGLQTDYFLIPVVDV
                     NGKLAIRPKAREVTDRYRAADTAG"
     gene            complement(552..>1098)
                     /locus_tag="PLY30_04490"
     CDS             complement(552..>1098)
                     /locus_tag="PLY30_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPW65400.1"
                     /translation="PLDETALIKRTEAVMQSVGYCIIVISETIRDHGGNRIGQKTEGV
                     TSDPFGHKYVEGTAARLASILEKNLGVRARFDKPGTIQRMSMPYISTVDQKEACQAGA
                     RAVAWALKGMSEVMVGFARKSGKRYAIEYRPVPLGRIPNNERYLPAEFFDAKHSMITP
                     AFAEYALPLLGPGLPEFPVFK"
BASE COUNT          225 a          318 c          333 g          222 t
ORIGIN      
        1 gaaggagaaa cgtaccggga tcgacgcgat caacgggatg gtcgtgcggc tcgggaaaaa
       61 gcacaaggtc ccgacgcccg tgaacgcgtt cctgagcccg tcgatcagaa aagccgaaag
      121 gtgttcatgg acgacaacgg gaacctcaag gggaaagaca gcatgagaca aaaaaccgtt
      181 atttgcgcgg ccgcgggcgt tcttctcgcg gtttttttga acggctgcgc ctcctctgcc
      241 cgctttgttg ccgttccctc gggaccgcta tctcaaaagc cctatgtcgc ggtctatggg
      301 aaagaggggt gtccggcctg cgctgacctt aaaaaagacc tggcccgaaa ggggacggac
      361 tatctcgata aggatatcag cgatcccggc atcaaacggg aactggtttc ccgaatggag
      421 accgcgggtc ttcaaacgga ttatttcctc atcccggtcg tggatgtgaa tggaaagctc
      481 gcgatccggc cgaaggcgcg cgaagtgacc gaccgttacc gcgctgccga taccgccggc
      541 tgacagctcc gttatttgaa taccgggaac tccggaagac cgggaccgag aagcggaagc
      601 gcgtactcgg caaaggccgg tgtgatcatg gagtgtttcg cgtcgaaaaa ctccgcgggg
      661 aggtagcgtt cgttgttcgg gattcttccg agcggcaccg gccggtattc gatcgcgtag
      721 cgtttcccgg atttccgcgc gaaccccacc atgacctctg acatcccttt cagagcccac
      781 gcgaccgccc gagctcccgc ctgacaggct tctttctggt caaccgtcga aatatagggc
      841 atggacatgc gctggatcgt gcccggtttg tcgaagcgcg cgcgcacgcc gaggttcttt
      901 tcaaggatcg aggcaagccg cgccgcggtc ccttccacgt atttgtggcc gaacgggtcc
      961 gatgttacgc cttctgtttt ctgcccgatc cggtttccgc cgtgatcgcg gatcgtctcc
     1021 gaaatgacaa tgatacagta accgaccgat tgcatgacgg cctccgtgcg cttgatcagg
     1081 gcggtctcgt cgagcggg
//
LOCUS       DAOOBX010000311         1094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_144141_length_1094_cov_3.861405, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000311 DAOOBX010000000
VERSION     DAOOBX010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144141_length_1094_cov_3.861405"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /gene="rph"
                     /locus_tag="PLY30_04495"
     CDS             complement(<1..537)
                     /gene="rph"
                     /locus_tag="PLY30_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPW65401.1"
                     /translation="MRIDGRRPGQLRKLAIRTDYLKFADGSCLIEMGETRVVCSASIE
                     EKVPPFCKSEGKGWVTAEYGMLPASCSQRIQRESSKGRPSGRTQEIQRLIGRSLRSVV
                     DLKKIGERTIWLDADVIQGDGGTRTASITGCYIALCLAVKKLLKSGVLTEDPVRDSVA
                     AISVGVVKDTPMLDLCYEE"
     gene            complement(548..>1094)
                     /locus_tag="PLY30_04500"
     CDS             complement(548..>1094)
                     /locus_tag="PLY30_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65402.1"
                     /translation="ADMTVREALAAVETLEAGSGPHSFVSLTGGEPLQQPDFLRGLTR
                     KLKECGYRVLLETNGICPRALDGTVELFDSISMDIKLSSVGRHPDFSDAHAEFLKIVR
                     RTETELCLKIVVAETMDFAEFDRHIGMIAEVAPETPVFIQQRQGERVDVPYLERAIQT
                     ARQRIADVRLGFQLHKMLNMK"
BASE COUNT          225 a          352 c          285 g          232 t
ORIGIN      
        1 ctcctcgtag caaagatcaa gcatcggcgt atcctttaca acccccacac tgatcgcggc
       61 aacagaatcc cggacagggt cttcggtcag gacaccgctc ttgagaagct ttttgaccgc
      121 aaggcaaagc gcgatgtagc aacccgtgat cgacgccgtt cgcgtgccgc cgtccccctg
      181 gatcacgtcg gcatcgagcc agatcgtccg ttcgccgatc tttttgaggt cgacgacgga
      241 ccggagactg cgtccgatca acctctggat ctcctgagtg cgtccggatg gcctgccttt
      301 cgaggactct ctttgaatgc gttgtgagca agacgccggc aacataccgt actcggccgt
      361 gacccaccct ttcccctccg acttgcaaaa aggcggaacc ttctcttcga tgctggcgga
      421 acaaacgacc ctggtttcgc ccatttcgat caggcaggat ccatccgcaa acttcaggta
      481 atccgtccgg atcgcaagtt ttctcagctg tccgggcctt cttccgtcga tcctcataat
      541 ctgcctccta tttcatgttg agcattttat gcaactggaa cccaagccgg acgtccgcga
      601 tccgttgccg cgccgtttgg atggcgcgct caagatacgg aacgtcaacg cgttccccct
      661 gcctctgctg gatgaaaacg ggggtttcgg gagcaacctc cgcgatcatg ccgatatggc
      721 ggtcgaactc cgcaaagtcc atggtttcgg caacgacgat cttgaggcag agctccgtct
      781 ccgtcctccg gacgattttc aggaattccg cgtgagcgtc cgaaaaatcc gggtgccgcc
      841 ccacgctcga gagcttgata tccatggaga tggaatcaaa caactcgacc gttccgtcaa
      901 gggcccgcgg acaaatccca ttcgtttcaa gcaggacccg gtatccgcat tctttcagct
      961 tccgtgtcaa cccccgcaga aaatccggct gctgaagcgg ttcgccaccc gtcaaactca
     1021 cgaaggaatg agggccggag ccggcctcca gggtttcgac cgcggcaagg gcctcccgaa
     1081 cggtcatatc cgcg
//
LOCUS       DAOOBX010000312         1089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_145194_length_1089_cov_2.392650, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000312 DAOOBX010000000
VERSION     DAOOBX010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145194_length_1089_cov_2.392650"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLY30_04505"
     CDS             complement(<1..211)
                     /locus_tag="PLY30_04505"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPW65403.1"
                     /translation="MFDSLISINDLSTDEIKKLFALAADLKARPQDFRNVLEGRMFGF
                     ISEKPSTRTWVSFQAGIFAMGGGVIY"
     gene            complement(196..>1089)
                     /locus_tag="PLY30_04510"
     CDS             complement(196..>1089)
                     /locus_tag="PLY30_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW65404.1"
                     /translation="GAIKFAKKYGGDTGRYEIITMKRSFHGRTIAAMTATGQDKFHQG
                     FEPLIEKFHYAEFNDLESVKKLLTPKTVAIMLEPIQGEGGIHVAKPEFMQGLRQLCDE
                     RDMLLILDEVQTGMGRTGKMFAYQHYHGVEPDIMTLSKALGGGVPIGAFVVNKRIRKE
                     VLTAGMHGSTFGGSPLVCAASLAVFKAIRKEKLLTNTVRMGELLKIEFEKLREKYPVI
                     EEYRGMALMCALKLSAPSAPYAQEARDRGLLVNSTQGDVLRFLPALNVTAKEVRRAVA
                     ILDQVFAKLSKKQEDCSCSTH"
BASE COUNT          213 a          339 c          302 g          235 t
ORIGIN      
        1 ggtagatcac accgcctccc atcgcaaaga tgcccgcctg gaacgaaacc caggtcctgg
       61 tcgacggttt ctcggaaatg aaaccgaaca tgcgtccctc gagaacgttc cggaaatcct
      121 gcggccgggc cttgagatcc gccgcaagtg cgaacaactt tttgatctcg tccgtgctga
      181 ggtcgttgat cgagatcaat gagtcgaaca tgagcagtct tcctgttttt tcgacagctt
      241 cgcgaatacc tggtcaagga tcgcgacggc cctgcggacc tctttcgcgg tcacgttgag
      301 cgccggcagg aaccgtagca cgtcgccctg ggtcgaattg accagaagcc cccggtcgcg
      361 cgcctcctgg gcgtagggcg cggacggtgc gctgagcttg agggcgcaca taagggccat
      421 gccgcgatat tcctcgatca cgggatactt ttcccggagc ttctcgaact cgatcttgag
      481 gagttccccc atccggaccg tattcgtgag cagtttttcc ttgcgaatcg ccttaaaaac
      541 ggccagggac gccgcgcaca cgagcgggct cccgccgaag gtcgagccgt gcatgccggc
      601 cgtcagaacc tcttttctga tccgtttgtt cacgacaaac gcaccgatcg gaacaccgcc
      661 gcccaaagcc ttcgaaagcg tcataatatc cggttcgacg ccgtggtaat gctggtaggc
      721 gaacatcttg cccgtccgcc ccataccggt ctgcacttca tccaggatga gaagcatgtc
      781 ccgctcgtcg caaagctggc gcaggccctg catgaattcc ggcttcgcga cgtggattcc
      841 gccctccccc tggatcggct cgagcatgat cgccacagtc ttcggcgtga gaagtttttt
      901 cacggactca agatcgttga actcggcgta gtggaacttc tcgatcagcg gctcgaaacc
      961 ctgatgaaac ttgtcctggc cggtcgcggt catcgctgcg atcgtccttc cgtggaagga
     1021 ccgcttcatc gtgatgatct cgtaacgacc ggtatcgcca ccgtactttt tggcgaactt
     1081 gatggcacc
//
LOCUS       DAOOBX010000313         1082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_146419_length_1082_cov_3.722493, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000313 DAOOBX010000000
VERSION     DAOOBX010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146419_length_1082_cov_3.722493"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1082
                     /locus_tag="PLY30_04515"
     CDS             <1..>1082
                     /locus_tag="PLY30_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HPW65405.1"
                     /translation="NKVDRPNARPNEVVDMTFDLFCELNSTEEQLDFPIVFASGKEGR
                     ATLDLDEESRDMKPLLDTILRHVPPPVADADQPFQMLVTMLDYDNYVGRMAIGRIFHG
                     SVKVGDSLALVKHEGHEVIRGRVTRILKYKGMVRVEAGSAEAGDIAAIAGIEEVHVAD
                     TLAHPDKPEAIPAVKIDEPTISMNFACNSSPLSGKDGGRFLTSRHIRERLQHEAMMNV
                     GVRIEDVPGEDRVKVSGRGELHLSILIETMRREGYELEVSRPEVILKEEDGQLFEPVE
                     EVVIEVDPGYQGSVIEALGVRKAEMKNLTTSAVGTIRMEFLIPSRGILGFRSQLLVMT
                     RGTGIMYQNFHDYQPFKGEIPERPSGV"
BASE COUNT          213 a          318 c          350 g          201 t
ORIGIN      
        1 aacaaggtcg atcgtccgaa cgcgcgtccc aacgaggtgg tcgacatgac cttcgacctt
       61 ttctgcgagc tgaactccac cgaggaacag ctcgattttc cgatcgtgtt cgcttccggc
      121 aaggaaggcc gggcgacgct ggacctggac gaggaatcgc gggacatgaa accgcttctt
      181 gatacgatcc tgcgccacgt gccgccgccg gtcgcggacg ccgatcagcc gttccagatg
      241 cttgtcacga tgctggatta tgacaactat gtcggccgga tggcgatcgg ccgcattttt
      301 cacggttccg tcaaggtcgg ggactccctc gcgctcgtga aacacgaagg ccatgaagtg
      361 atccggggcc gtgtgaccag gatcctgaaa tacaaaggca tggtccgcgt tgaggccgga
      421 tcggccgagg ccggcgatat cgccgcgatc gcggggatcg aggaagtgca tgtggccgac
      481 acgctcgcgc atcccgacaa gccggaggcg atcccggcgg tcaagatcga cgagccgacc
      541 atctcgatga atttcgcctg taattcgagc ccgctttccg gcaaggacgg cggccggttc
      601 ctgacctcgc ggcacatccg cgagcgtctt cagcacgagg ccatgatgaa tgtcggcgtg
      661 cggatcgagg atgttccggg cgaggaccgc gtcaaggtct cgggccgcgg cgagctccac
      721 ctttccatcc tcatcgagac gatgcgccgg gaaggttacg agcttgaggt ctcgcgtccg
      781 gaggtcattc tcaaggaaga ggacggacag ctttttgaac cggtcgagga ggtcgtgatc
      841 gaggtggacc ccggctacca gggctctgtc attgaagcgc tcggtgtgcg caaggccgag
      901 atgaagaacc tgaccacgag cgccgtcggg acgatccgca tggaattttt gatcccgtcg
      961 cgcggcattc tcggcttccg aagccagctt ctcgtgatga cgcgcggaac ggggatcatg
     1021 taccagaact ttcacgacta ccagccgttc aagggcgaga ttccggaacg gccgtcggga
     1081 gt
//
LOCUS       DAOOBX010000314         1078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_147266_length_1078_cov_2.556207, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000314 DAOOBX010000000
VERSION     DAOOBX010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147266_length_1078_cov_2.556207"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..>1078)
                     /locus_tag="PLY30_04520"
     CDS             complement(38..>1078)
                     /locus_tag="PLY30_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPW65406.1"
                     /translation="ILAEQHYILMKDRVKTFPVTVELLSRFQTAAGQKAVVRSLKTGA
                     TDIVIGTHRLLSKDIEFRELGLVVIDEEQRFGVRHKEAFKRMRSQVDVLTLTATPIPR
                     TLHMALLGVRDMSVIDTPPRHRMPVRTFVMEYHENIVKQAVEREIARKGQVYFIHNRV
                     ENIEKVHQKLKELMPGVRFGVAHGQMPVEMLERVMMSFIKGSVDCLISTNIIESGIDI
                     PNVNTLIVNRADCFGLADLYQLRGRVGRFREPRQAYAYFLTPKNWVLTLDAGKRLAAI
                     ERFVELGSGFRIAMEDLEIRGAGNLLGHEQSGFIHAVGFDLYCKMLKRAVEEQRTSAR
                     SGTPSTEQRSPA"
BASE COUNT          208 a          328 c          307 g          235 t
ORIGIN      
        1 gtacgaagta cggagtacgg tgcatgctgt aaccgcttta cgcgggcgat cgttgttccg
       61 tgctcggcgt accggacctc gcgcttgttc tttgctcttc caccgctctc ttcagcatct
      121 tgcaatagag gtcgaatccg accgcgtgaa tgaacccgct ctgttcgtgc cccagcaaat
      181 ttccggcgcc gcggatctcg aggtcttcca tcgcgatccg gaaccctgag ccgagctcca
      241 caaaccgttc gatcgcggca aggcgcttcc cggcgtcaag cgtcaaaacc cagttcttcg
      301 gcgtcaggaa atacgcgtag gcctggcgcg gctccctgaa acggccgaca cggccgcgaa
      361 gctggtagag gtccgcgagg ccgaaacaat cggcgcggtt cacgatcagc gtgttcacat
      421 tcgggatatc gatcccggac tcaataatgt tggtcgagat caaacagtcg accgaccctt
      481 ttatgaagct catcatgacc cgttcgagca tctcgaccgg catctgcccg tgcgcgacgc
      541 cgaagcggac cccgggcatg agttctttca gtttttggtg gaccttttcg atgttctcga
      601 cgcggttgtg gatgaaatag acctggccct tcctggcgat ctcacgttcc acagcctgtt
      661 tcacgatatt ttcgtgatat tccatcacaa atgtccggac cggcatgcgg tgacgcggcg
      721 gggtgtcgat cacggacata tcacggacac cgagcaaggc catgtggagc gtcctcggga
      781 tcggtgtcgc agtcagcgtc aggacgtcca cctgcgaacg catccgtttg aaggcttcct
      841 tgtgccggac accgaagcgc tgttcctcgt cgatcacgac gagcccaagc tcccggaact
      901 cgatatcctt cgacaagagc cggtgggtac cgatcacgat gtcggtcgct cccgtcttga
      961 gggaacgcac gactgccttc tgcccggccg cggtctggaa cctcgaaagg agctcgaccg
     1021 tcacgggaaa ggttttcacg cgatccttca tcaggatgta gtgctgttcg gcgaggat
//
LOCUS       DAOOBX010000315         1074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_148251_length_1074_cov_1.575074, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000315 DAOOBX010000000
VERSION     DAOOBX010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148251_length_1074_cov_1.575074"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1074
                     /locus_tag="PLY30_04525"
     CDS             <1..>1074
                     /locus_tag="PLY30_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65407.1"
                     /translation="IRLPDIHSDKSADRARAEAGFYLSNTLSHTIRYRLLSYGKRPDL
                     TRINYVYNSIPLAIRRLEVLSHQPAFEPEYLHDPLFTELKLFEDHPPPDLPLREYLGD
                     QAYLEQFIGRRLSRELKEFGNVARRDERAFLMSALLFKVRSLNKNTARVIRIVEKQAS
                     ESGDPMGAKREADEKISRMRRNLELYENLQPLIKPLFGPESPDPAFVQRLISGLGRAL
                     DDSLELLKILREDRLRGQSMLYGIHVAEKKRYAAWHKEDEERGARMIRDNDTRAASPD
                     AATLLAAAGASEGKIPANRQFAGAGAVERPLRVSVVIGATLGDDALTDQLRHYLAAPG
                     SSKIQKQIFVAVDLRDRELHPKRP"
BASE COUNT          231 a          282 c          328 g          233 t
ORIGIN      
        1 catccggttg cccgacatcc attcggacaa gtctgccgac cgggcccgtg ccgaggcggg
       61 attttatctt tccaacacgc tgtcgcatac catacggtac agacttttga gctacgggaa
      121 acgcccggat ctgactcgta tcaattacgt gtacaattcg ataccgctcg cgatcaggcg
      181 actggaggtc ctttcgcacc aacccgcctt tgagccggaa tacctgcatg atcccctgtt
      241 tacggaactc aagctctttg aagatcatcc gccgccagac ctcccgctgc gggaatatct
      301 cggggatcag gcttatcttg aacaatttat cgggagacgt ctttcgcggg aattaaaaga
      361 gttcgggaat gttgcccgaa gggacgaaag ggcgtttttg atgtcggctc ttttattcaa
      421 ggtccgcagt ctgaataaaa acaccgcgcg agtgatccgg atcgttgaaa agcaggcttc
      481 ggaaagcggg gaccccatgg gggcgaaaag ggaggcggac gaaaagattt cccgcatgcg
      541 acgaaatctt gagctttatg aaaacttgca gccgctgatt aaaccgcttt tcggcccgga
      601 gagtcccgac cccgcttttg tgcagcgtct gatctcaggg cttgggcgag ccctcgatga
      661 ttcgctcgag cttttgaaga ttcttcggga ggatcgtttg agggggcagt ccatgttgta
      721 cggaatccat gtcgcggaaa agaagcgtta cgcggcgtgg cacaaggagg atgaagagcg
      781 tggagcacgg atgatccgcg acaatgatac ccgcgcagcg tcgccggacg cggcgacgct
      841 gctggcggcg gcgggcgctt cggaagggaa aattccggcg aaccgtcaat tcgccggggc
      901 gggggcggta gaacgcccgt tgcgggtttc ggtggtgatc ggcgctacgc ttggtgatga
      961 tgcgctgacg gatcagttgc ggcattatct tgccgcgccc ggaagttcga agatccagaa
     1021 acaaatcttt gttgcggttg atcttcgcga tcgcgagctt cacccgaagc gccc
//
LOCUS       DAOOBX010000316         1060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_150990_length_1060_cov_2.484577, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000316 DAOOBX010000000
VERSION     DAOOBX010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150990_length_1060_cov_2.484577"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLY30_04530"
     CDS             <1..275
                     /locus_tag="PLY30_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPW65408.1"
                     /translation="SAVTAVFQETKFDLAILDIRMGGCGGYGVCSVAKNSPKCRHIPV
                     ILMSAIEKDSLDWKRVKADAFMLKPFETERLVGLVRECLRKGQTGG"
     gene            280..705
                     /locus_tag="PLY30_04535"
     CDS             280..705
                     /locus_tag="PLY30_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPW65409.1"
                     /translation="MRIVTSRIIRRTKGFSDIHDLTAEARLCVEKSGISNGQALFFVV
                     GSTAGMTTVEFEPGLVKDLKELFERIAPRAAAYHHEETWHDGNGFSHVRASLLKPSLI
                     VPIVDGSMTLGTWQQVVLVDFDNTPREREVVLQITGEAT"
     gene            702..>1060
                     /locus_tag="PLY30_04540"
     CDS             702..>1060
                     /locus_tag="PLY30_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPW65410.1"
                     /translation="MIVVKRGDITLEDVDVIVNAANTGLRGGGGVDGAIHRAAGPSVM
                     EECRRIGGCSTGQAVLTAAGKLKAKKIIHTAGPVWHGGRRREPELLRDSYRNSFRLAS
                     ENGLRTIAFPAISTGVYG"
BASE COUNT          220 a          252 c          365 g          223 t
ORIGIN      
        1 tctctgctgt tactgctgta ttccaggaaa ccaagttcga ccttgctatc ctggatatac
       61 gaatgggggg gtgcggcggt tatggcgtct gttccgtcgc caaaaattcc ccgaaatgca
      121 gacatatccc ggtaatcctt atgagcgcga tcgaaaagga ctcgcttgac tggaaaaggg
      181 tcaaggcgga cgcgttcatg ctgaagccgt tcgagacgga gaggctcgtt ggtctggttc
      241 gtgaatgcct gcggaagggg cagaccgggg gttgacggga tgcggatcgt cacgtcgcgg
      301 atcattcgca ggacgaaggg gttttcggat attcatgacc tgacggccga agcgcgactg
      361 tgcgttgaaa aaagcggtat ctcgaacggg caggcgcttt tctttgtcgt cggatcgacg
      421 gcggggatga cgacggtgga gtttgaaccg ggactcgtga aggatctgaa ggagcttttt
      481 gaaaggatcg ccccccgcgc cgcggcgtat catcatgaag agacctggca cgacggaaac
      541 ggtttctccc atgtcagggc ttcgcttctg aagccatccc tgatcgtgcc gatcgtggac
      601 ggaagcatga cgctcgggac ctggcagcag gtcgtactcg ttgattttga taatactccg
      661 cgcgagcgag aggtcgtttt gcagatcacc ggggaagcga cgtgatcgtc gtcaaacgcg
      721 gggacattac gctcgaggat gtcgatgtga tcgtgaatgc cgctaatacg ggtttgcgtg
      781 gcggaggagg cgttgatgga gccatccatc gagcggccgg gccgtccgtg atggaagagt
      841 gcaggcggat cggcggctgt tcgacgggac aggcggtcct gacggcggcg ggaaagttga
      901 aggccaagaa gatcatccat acggcaggtc ctgtgtggca tggcggtaga cggcgggaac
      961 ccgagttatt gcgggattca tacaggaaca gtttcaggct ggccagcgag aacggtttgc
     1021 ggaccatcgc gttcccggcg atcagcacgg gtgtttacgg
//
LOCUS       DAOOBX010000317         1049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_153230_length_1049_cov_2.380282, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000317 DAOOBX010000000
VERSION     DAOOBX010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153230_length_1049_cov_2.380282"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..>1049
                     /locus_tag="PLY30_04545"
     CDS             191..>1049
                     /locus_tag="PLY30_04545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW65411.1"
                     /translation="MTHIKTDILVIGGGPGGYTAAFHAAVKGKKVVLVEKEPRLGGVC
                     LNCGCIPSKALLHAAETVLGAKEAEKFGVRFPEPSLDLDKLRAWKSGILEKLGRGITG
                     LAGRRGVEIVRGEAVFQDARTVSAGGIVIGFDHAIIATGSVPAVPKAFRDGGPGVMTS
                     TEALELRDIPGRLLVIGAGYIGMELGTAYAALGSRVTVVEALPAMLNGADADLVRYVE
                     EPARKRFEKICLNARVVSLADAKDRVKAVIETDGKTLEEDFDRVLVSVGRVPFTGGLA
                     LERTAVRSDE"
BASE COUNT          189 a          289 c          368 g          203 t
ORIGIN      
        1 ggtttcgggg atcgcgaaac ggcacttccc ggatttcagg aaacggttcg cgaccccgag
       61 actcaggctt ttgaaggtta tgacgggacg gagttgggcc tcggcgctct atcgcaggcg
      121 atccgctcaa gaaagccgtt cccttgcccg cttcttttgt tagaatatcc cgcattaacc
      181 cggaaatttt atgacccata tcaaaacgga cattcttgtg atcggcggcg gtcccggcgg
      241 ctacacggcc gccttccacg cggccgtcaa ggggaaaaag gttgttcttg tcgagaaaga
      301 accccggctt ggcggcgtgt gcctgaactg cgggtgtatc ccgtccaagg cgctgttgca
      361 cgcggcggaa acggtcctcg gcgcgaagga ggccgaaaag ttcggtgtcc gtttcccgga
      421 accgtcgctg gatctcgaca aactccgcgc ctggaaatcg gggatccttg aaaagcttgg
      481 ccgggggatc acggggctcg cgggacggcg gggggttgag atcgtccgcg gtgaggctgt
      541 gtttcaggac gcgcggacgg tcagcgccgg cggcatcgtg atcggtttcg accacgcgat
      601 catcgcgaca ggatccgttc cggccgttcc gaaagcgttc cgggacggcg gtccgggagt
      661 gatgacttcg actgaggcgc tggaactgcg ggatatcccc gggcggctgc tcgtgatcgg
      721 cgcggggtac atcggcatgg aactcgggac ggcctacgcg gcgctcggga gccgggtgac
      781 ggtcgtggag gcacttccgg cgatgctgaa cggggcggac gcggaccttg tccggtacgt
      841 cgaagaaccg gcccggaaac gttttgagaa gatttgcctg aacgccaggg tcgtgagcct
      901 tgcggatgcg aaggatcgcg tgaaggccgt gatcgaaacg gacggcaaga cgcttgaaga
      961 ggattttgac cgcgtgctcg tttcggtggg tcgcgtgccc ttcaccggcg gcctcgctct
     1021 tgaaagaacg gcggttcggt ccgatgaac
//
LOCUS       DAOOBX010000318         1046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_153942_length_1046_cov_1.915237, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000318 DAOOBX010000000
VERSION     DAOOBX010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153942_length_1046_cov_1.915237"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1046
                     /locus_tag="PLY30_04550"
     CDS             <1..>1046
                     /locus_tag="PLY30_04550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPW65412.1"
                     /translation="HQSLTKEKVLELEPGVKREGLKGGVLYYDAQMDDARLCLENVLS
                     AAEAGAHVANYAEATGFLKANGRAEGVKAVDVLSGAEFEVRAKRIVVAGGPWTNGLLR
                     LDDDHAKKKVRTTKGIHIVYKDRLSDRALFIPSASDKRIFFVIPWLGHSLIGTTDTDY
                     VGNPDQAGAEPEDVDYLMRETKRVFPRLDVSPAKILSTFAGLRPLVRKGGSANKVSRK
                     HLFHESPSGIIFVVGGKYTTYRAVAEDCLRRIFKDFRPVKPFTVYGGGMVAENAEVIA
                     DGCGLEPGIVRSLMDLYGGRYRDVLALVEKAPSLAEKVSDDPPVIKAQLGYAVEAEMA
                     RKPEDVFMRRLPLG"
BASE COUNT          218 a          274 c          333 g          221 t
ORIGIN      
        1 tcaccaaagc ctcacgaaag aaaaagtcct cgaactcgaa cccggtgtga aacgcgaggg
       61 cctgaaaggc ggcgtcctct actatgacgc gcagatggac gacgcgaggc tttgtcttga
      121 gaacgtcctt tccgccgccg aggcgggcgc tcatgtcgcc aattacgcgg aggcgacggg
      181 gtttctcaaa gcgaacggac gcgcggaggg tgtgaaagcg gttgatgttc tttccggagc
      241 ggaatttgaa gttcgcgcga agagaatcgt tgttgcggga gggccgtgga cgaacgggct
      301 tctcaggctt gatgacgatc acgcgaaaaa aaaggtccgg acgaccaaag gcattcatat
      361 cgtatataaa gaccggctct cggatcgggc gctttttatt ccttccgcaa gcgacaagcg
      421 gatttttttc gtgatcccgt ggctcgggca ctcgttgatc ggtactacgg acaccgatta
      481 cgtcggaaat cccgatcaag ccggggcgga gccggaggat gtggattatc tgatgcggga
      541 aacgaagcgg gtatttccac gactcgatgt ttccccggcc aaaatcctct cgaccttcgc
      601 ggggctccgg ccgctggtca ggaagggcgg ctccgcgaac aaggtctccc gcaagcacct
      661 gttccatgaa tccccgtcgg gcattatttt tgtggtgggg gggaagtata cgacatatcg
      721 ggcggttgct gaagattgcc tcaggagaat tttcaaggat ttccgtccgg tgaaaccttt
      781 cacggtttac ggcgggggaa tggttgcgga aaacgcggag gtcatcgccg acggctgcgg
      841 tcttgagccc gggatcgttc gttccctcat ggatctctac ggcggccggt accgcgacgt
      901 gcttgccctg gttgagaaag ctccgtccct cgcggaaaag gtctccgacg atccgcctgt
      961 gatcaaagca cagcttggct acgccgtgga agccgagatg gcgcgaaagc cggaagatgt
     1021 tttcatgcgc cggttgccgc tgggtt
//
LOCUS       DAOOBX010000319         1042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_154746_length_1042_cov_2.427558, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000319 DAOOBX010000000
VERSION     DAOOBX010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154746_length_1042_cov_2.427558"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PLY30_04555"
     CDS             <1..965
                     /locus_tag="PLY30_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPW65413.1"
                     /translation="NTMPQWAGSCWYYLRYIDPTNPEVFVAREKESYWMPVDLYVGGA
                     EHAVLHLLYSRFWHKVLFDLGLVSTPEPFMKLVNQGMILGEDNQKMSKSRGNVINPDD
                     VVKQYGADSFRLYEMFMGPLEMTKPWSTAGVEGVYRFLGKVWRLFIDREGRLHANIVS
                     RVPDASELRSLHRAIKKVTDDLEELRMNTAISALMIFVNEVSSHADHSREILEKFLLL
                     LAPFAPHLAEELWLMLGHDKTLAYAEWPGYDAKYLVEDEVEMAILVGGKVRTKMTVPS
                     ALGEEEVKKMAVEQPRIREFLAGKTVVKIIVVPKRTVNLVVKDA"
BASE COUNT          228 a          275 c          316 g          223 t
ORIGIN      
        1 cgaacacgat gccgcagtgg gcgggttcct gctggtatta cctgcggtat atcgatccga
       61 cgaacccgga ggtctttgta gccagggaaa aggaaagcta ctggatgccg gtcgatcttt
      121 atgtcggcgg cgccgagcac gcggtgctcc acctccttta ttcgcgtttc tggcacaagg
      181 ttctctttga cctgggcctt gtatcaacac ccgagccgtt tatgaagctt gtcaaccagg
      241 gcatgatcct cggcgaagac aatcagaaga tgtcgaagtc ccgcggcaat gtaattaatc
      301 ccgatgatgt cgtgaaacag tacggcgcgg actcattccg gctgtacgaa atgttcatgg
      361 ggccccttga gatgacgaag ccgtggtcca cggccggggt tgagggcgtt taccggttcc
      421 tcgggaaagt ctggcgactt tttatcgata gggaggggcg cctccacgcg aatatcgtga
      481 gccgggtccc ggacgcgtcc gaactgagat ctctccaccg ggcgatcaag aaggtgacgg
      541 acgatctcga ggaacttcgc atgaacacgg cgatctcggc ccttatgatc ttcgtgaacg
      601 aggtgtcatc ccacgcggac cactcgcggg agattctcga aaagttcctg ttgcttctcg
      661 cgccgttcgc cccgcacctt gcggaagaac tctggctgat gctcggccat gacaagacgc
      721 ttgcttatgc ggagtggccc gggtatgatg cgaaatatct cgttgaggat gaagttgaaa
      781 tggcgatcct cgtcggcggg aaggtccgta cgaagatgac cgttccttcc gcgctcggcg
      841 aagaagaagt gaaaaagatg gccgtggagc agccccgtat ccgggagttc ctcgccggca
      901 aaacggtcgt caagatcatc gtggtgccga aacgaaccgt gaacctggtt gtgaaggatg
      961 cctaaagtcg agatcaaaca aagcaggatc ctggtcggcg gtaaagaagt cccgctcatt
     1021 tccggagagg tccattactg gc
//
LOCUS       DAOOBX010000320         1041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_154984_length_1041_cov_2.351927, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000320 DAOOBX010000000
VERSION     DAOOBX010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154984_length_1041_cov_2.351927"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PLY30_04560"
     CDS             <1..680
                     /locus_tag="PLY30_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPW65414.1"
                     /translation="VKKFVETRELTVFFVVDMSGSQHFGTSGKLKSEIAAEIAAILAF
                     SAVKNNDKTGLLICTDRVEKFLPVKKGRNHALRVIREILGYQPQSRGTKLESAIQYIH
                     NVLTRSAVVFLISDFQDEGYEKALKILSQKHDVVAIHLKDRLERSFPGVGLVELRDRE
                     TGETTLIASASRTFRKRFEENTRAREESTSKLFRKLQIDRVEVPAEASYIDPLIKFFK
                     SRERKRK"
     gene            677..>1041
                     /locus_tag="PLY30_04565"
     CDS             677..>1041
                     /locus_tag="PLY30_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65415.1"
                     /translation="MNRTLHRTVGPAAFLAILLLAPGFLRAEDDIAVRAEVNKAFLTI
                     GDPVTYTVTVTHAPEIKILNRIPAPDSGILEVKKIEEIHRTEKKKIVTGRRFTLTTYR
                     LGEFVLDPVDINYKAGEGA"
BASE COUNT          243 a          323 c          283 g          192 t
ORIGIN      
        1 tcgtcaagaa gttcgtcgag acgcgcgaac tcacggtctt tttcgtcgtc gacatgtcgg
       61 gatcgcagca cttcggtacc tccgggaaac taaaatccga aatcgcggcc gagatcgcgg
      121 ccatcctggc attttccgcg gtcaagaaca acgacaagac cggccttctc atctgcacgg
      181 atcgcgtcga gaaattcctc cccgtcaaga aagggcgcaa ccacgcgctc cgcgtcatcc
      241 gcgagattct cggttaccag ccgcaaagcc gcggcacgaa gctcgagagc gcgatccagt
      301 atattcataa cgtcctcacg cgctccgccg tcgtctttct catctcggac tttcaggacg
      361 agggttatga aaaggccctc aagatcctga gccagaagca cgacgttgtc gccatccacc
      421 tgaaggaccg cctggaacgg tcgttcccgg gcgtcggact ggtcgagctc cgggaccgcg
      481 agacggggga aaccacgctc atcgcttcgg cttccaggac gttccggaaa cgcttcgaag
      541 aaaacacgcg ggccagggag gaatccactt cgaaactttt cagaaaactc cagatcgaca
      601 gggttgaggt gccggcggaa gcctcctaca tcgacccttt gatcaaattc ttcaaaagca
      661 gggagcggaa acgcaaatga accggacgct gcatcgcacg gtgggtcccg ccgcctttct
      721 ggcaatcctg cttcttgcgc ccggctttct gcgcgcggaa gacgatatcg ccgtccgcgc
      781 ggaggtcaat aaagccttcc tcacgatcgg tgatccggtc acctacaccg taaccgtgac
      841 ccacgctccg gagatcaaga ttctgaaccg cattcccgcg ccggattccg gaatcctcga
      901 ggtcaaaaag atcgaggaga tccatcgaac ggaaaagaag aagatcgtca ccggccgccg
      961 ttttacactc acgacctacc gactcggaga attcgttctc gatcccgtgg acatcaacta
     1021 taaggcgggg gagggggccg a
//
LOCUS       DAOOBX010000321         1039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_155343_length_1039_cov_3.882114, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000321 DAOOBX010000000
VERSION     DAOOBX010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155343_length_1039_cov_3.882114"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLY30_04570"
     CDS             complement(<1..744)
                     /locus_tag="PLY30_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPW65416.1"
                     /translation="MDTQTAIEKKPGDRNHFLIGEFLNYLAVEKGLSQNTLDAYRRDL
                     DHYTGFLRREKMPDWEGITRAHILKFLAAEGRRGLEVPSVARALVSVKLFHRFLAHEK
                     YVKEDVTSVLESSRQWKKLPQFLTVQEMDAILKAPDPKTPAGIRDRALLECLYATGMR
                     VSEVTGLSAGNVYLDNHFIKCRGKGNKERIVPLGSVAVDACRAYLARVRSKIKARTDH
                     FFLGRNGSGLTRQYVWQMIKRYAMRAGIAK"
     gene            complement(850..>1039)
                     /locus_tag="PLY30_04575"
     CDS             complement(850..>1039)
                     /locus_tag="PLY30_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPW65417.1"
                     /translation="AEAKRFVEKLLPPGTRISIKTMRSDKYDRYLADVFAADAESGED
                     KFLNGWLIEKGLAVKVSE"
BASE COUNT          188 a          319 c          303 g          229 t
ORIGIN      
        1 cttcgcgatc ccggcgcgca tcgcgtagcg tttaatcatc tgccagacat actgacgtgt
       61 cagccccgag ccgttccgtc cgaggaagaa atgatccgtc cgcgccttga tctttgaacg
      121 cacgcgcgcg aggtaggctc ggcaggcgtc gaccgcgacg ctgccgagcg ggacgatccg
      181 ttccttgttc cctttcccgc ggcacttgat gaaatgattg tccaggtaaa cgttcccggc
      241 gctgaggccc gtcacttcgg aaacgcgcat gccggtcgcg tacaggcatt cgagaagcgc
      301 ccggtcgcga atcccggccg gtgtcttcgg gtccggtgcc ttcaggatcg cgtccatctc
      361 ctgcacggtg aggaactgcg gcagtttctt ccattgtctg gaagactcga gcacgctcgt
      421 cacatcctct ttcacgtact tctcgtgcgc gaggaaacgg tgaaaaagct tgaccgagac
      481 gagcgccctc gcgacagacg ggacctcaag cccgcggcgg ccctcggcgg cgaggaactt
      541 caggatatgc gcgcgggtta ttccctccca gtccggcatc ttttcccggc ggaggaatcc
      601 cgtgtaatga tcgaggtcgc ggcggtaggc gtcgagcgtg ttctgcgaga ggcctttttc
      661 gacggcaagg tagttcagga actccccgat gaggaaatga ttccggtccc cgggtttttt
      721 ttcgatcgcg gtttgggtat ccatcaaaaa aattatcggc caccggagcc aaaccctaga
      781 gcgccgccat cccggaaaaa cggggtcaga ccccgttttt cagcaggacc ccggccatca
      841 ttttctgttt tattcgctca ctttcacggc gagccctttt tcaatgagcc agccgttcag
      901 gaacttgtct tcgccgctct cggcgtcggc cgcaaaaaca tcggccaggt agcggtcgta
      961 tttgtcggac ctcatggtct tgatcgagat tcgcgtgccg ggcgggagaa gtttttcgac
     1021 gaaccgcttc gcctccgcg
//
LOCUS       DAOOBX010000322         1038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_155588_length_1038_cov_3.557477, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000322 DAOOBX010000000
VERSION     DAOOBX010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155588_length_1038_cov_3.557477"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLY30_04580"
     CDS             complement(<1..393)
                     /locus_tag="PLY30_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPW65418.1"
                     /translation="MKKIVLAYSGGLDTSCAVKWLQEHYGYEVICFSAFIGEVKDKTK
                     LIKRAKAAGALKTFIVDLKDEFAKDYILPAIWAHARYEGKYPLATALGRPMIAKHLVE
                     VAEREGAEAVAHGCTGKGNDQVRLEVGVR"
     gene            complement(463..>1038)
                     /locus_tag="PLY30_04585"
     CDS             complement(463..>1038)
                     /locus_tag="PLY30_04585"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPW65419.1"
                     /translation="VINGLSDKEHPCQALADYMTLYEKIPAEKDPVLAFVGDGNNVLH
                     SLILVAAKLGAKIRYATPKKYQPDAKIVRTAREIAKKTGAVIEGFFDPARAVAGADAI
                     YTDVWVSMGEEAMRKKKLKDFQGMQVNRELVAKARKNVLIMHCLPAHRGEEITDSVME
                     SKNCIVFDETENRLHIQKAVLLHLFNEGAIQ"
BASE COUNT          182 a          323 c          277 g          256 t
ORIGIN      
        1 cctcaccccc acttcaaggc ggacctgatc gttgcccttg cccgtgcaac cgtgcgccac
       61 ggcctcggcg ccctcgcgtt cagcgacctc gaccaggtgc ttcgcgatca tgggacgccc
      121 gagcgcggtc gcgagcggat atttcccctc gtaacgcgcg tgggcccaga tcgcgggaag
      181 gatgtagtcc ttcgcgaact cgtccttgag atcgacgatg aaggtcttga gcgcgccggc
      241 ggctttcgcg cgcttgatca gcttggtctt gtccttgact tccccgatga acgcggaaaa
      301 acagatgacc tcgtagccgt aatgttcctg cagccatttc acggcgcagg aagtgtcaag
      361 tccgcctgaa tatgccagca ctattttttt cattgtttca tccttttcaa tgactgaagg
      421 ccggaaatgt tccggttttt caaaaatcga tcgtccccag ccttattgga tcgctccttc
      481 gttaaagaga tgaagcaaaa cggccttctg gatgtgaagc cggttctcgg tttcgtcgaa
      541 tacgatgcag ttcttgcttt ccatgacact gtcggtgatc tcctcgccgc ggtgcgccgg
      601 caggcagtgc atgatgagca cgtttttcct ggccttcgcg acgagctccc tattgacctg
      661 catgccctga aagtccttca gcttcttctt ccgcatcgcc tcttcgccca tactgaccca
      721 gacatcggtg taaatcgcat ccgctccggc cacggccctg gcggggtcaa agaacccttc
      781 gatcacggcc cccgtctttt tcgcgatttc ccgcgcggtc ctcacgatct tggcgtcggg
      841 ctggtatttc ttcggcgtcg cgtaacggat cttcgcgccc agtttcgccg cgacaaggat
      901 cagcgagtgc aggacattgt tgccatcccc tacgaacgcg aggacggggt ctttttcggc
      961 ggggatcttc tcatacagcg tcatgtaatc cgcaagcgcc tggcagggat gctccttgtc
     1021 gctcaagccg ttgatgac
//
LOCUS       DAOOBX010000323         1035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_156228_length_1035_cov_3.280612, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000323 DAOOBX010000000
VERSION     DAOOBX010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156228_length_1035_cov_3.280612"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PLY30_04590"
     CDS             <1..931
                     /locus_tag="PLY30_04590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW65420.1"
                     /translation="RYPFLGLGSENYRQEILENYGGVNLMKGAIFFADHISTVSPTYA
                     GEILSEPGGNGLSEYLNRRRDDVSGVLNGADYDHWDPSTDKLIPAHYSAEDLRGKAVC
                     KHALQTEFHLENAANVPVIGIITRFAPQKGLQNLMPVIVRLLRDTKAQFVILGTGEKW
                     QEDFFGGLPAVHHGRVGAWIGYSNRKAHLIEAGSDFFLMPSLYEPCGLNQMYSLRYGT
                     LPIVRATGGLRDTVIQYDERTGAGTGFLFYDATPEAIYGTVRWAVSTYFDRPRHIEMM
                     RKLAMKQRFSWHDSAKRYEEIYRRARERRASWH"
BASE COUNT          202 a          324 c          299 g          210 t
ORIGIN      
        1 ccggtacccg ttcctcgggc tcggttcgga aaattacagg caggagattc tcgaaaacta
       61 cggcggcgtt aacctgatga aaggcgcgat ctttttcgcg gaccacatct ccaccgtcag
      121 tcccacgtat gccggggaga tcctttccga gccgggcggc aacggccttt ccgaatatct
      181 gaaccggcgc cgcgacgatg tgtccggcgt cctgaacggc gccgattacg accactggga
      241 tccttcgacc gacaagctga tcccggcgca ttattccgcc gaagaccttc gcggcaaggc
      301 ggtctgcaaa cacgctcttc agacggagtt tcatctcgag aacgcggcga acgttcccgt
      361 gatcggaatc atcacaaggt tcgctcccca aaagggtctc cagaatctca tgccggtgat
      421 cgtccggttg ctccgcgaca cgaaagcgca gttcgtgatc ctcgggaccg gcgaaaaatg
      481 gcaggaggat ttcttcggcg ggctgcccgc cgttcatcac ggacgcgtcg gggcctggat
      541 cggatacagt aacaggaagg cccatctgat tgaggcgggt tccgatttct tcctcatgcc
      601 gtcgctctac gagccctgcg gcctgaacca gatgtacagc ctccggtacg gaacgctccc
      661 gatcgttcgc gcgacgggcg ggctccggga cacggtcatc cagtacgatg aaaggacggg
      721 cgccggtacg ggattcctct tttacgatgc cactccggaa gcgatctacg ggaccgtgcg
      781 ttgggccgtc agcacttact ttgaccgccc gcggcacatc gaaatgatgc gaaagctcgc
      841 gatgaaacag cgtttttcct ggcacgattc cgcgaaacgc tacgaagaga tttaccgccg
      901 cgcccgcgag cggcgtgcct cctggcatta aaccgtccgg tcatgttttc cctgtttccg
      961 cccccgggct ctgaatctgt tcgctgactc cgtccgcgct ttttcaggga aaccagcggc
     1021 ggaccacgaa ggttt
//
LOCUS       DAOOBX010000324         1033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_156697_length_1033_cov_3.127812, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000324 DAOOBX010000000
VERSION     DAOOBX010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156697_length_1033_cov_3.127812"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLY30_04595"
     CDS             <1..536
                     /locus_tag="PLY30_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65421.1"
                     /translation="KGFDSEFMKMFLEVLAQRGVVNEVAVSGQLNGYQKQLTALKIAT
                     RSVRRLGDPFIPRNSQNAVPMIMNTDDRSVVDLLNEIFSPVMSDAEEVADPYVREFTN
                     VLKIVAAIHIADLSKRIKDPGMLKAELLKRLNIAERTELQNLIRMDASGRGFTVSSAV
                     VRIFLRMRAEESIAQAA"
     gene            complement(559..>1033)
                     /locus_tag="PLY30_04600"
     CDS             complement(559..>1033)
                     /locus_tag="PLY30_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPW65422.1"
                     /translation="KITPKSYHQANGFNFDPILEVAILFLGIFITMQPALEILYLRGA
                     ELGIKQPWQFFWATGMFSSFLDNAPTYLTFISLAQGLGLSGPYLNIPEKLLAAISIGS
                     VFFGAMTYIGNAPNFMVRSIAHQRGWKMPSFFGYMAWSAAILLPLFLLVTLIFFR"
BASE COUNT          257 a          254 c          327 g          195 t
ORIGIN      
        1 cgaaaggatt tgattccgag tttatgaaaa tgttcctgga ggtcctggcg caacgcgggg
       61 ttgtcaacga agtggcggtg agcggacaac tgaacggcta tcagaaacag ctgacggcgc
      121 tcaagatcgc gacgcgttcc gtcaggcgcc tcggcgatcc ctttattccg aggaattccc
      181 agaacgcggt cccgatgatc atgaacacgg atgaccggag cgtggtggat cttctcaatg
      241 aaatcttcag ccctgtgatg tcggatgcgg aagaggtcgc tgatccatac gtcagagagt
      301 tcacgaatgt cctgaaaatc gttgcggcga tccacatcgc ggatctttcg aagcggatca
      361 aggaccccgg gatgctgaag gcggagcttc tgaagcggct caatatcgcg gagcggacgg
      421 agcttcagaa cctgatccgg atggacgcct caggccgcgg attcacggtc tcgagcgcgg
      481 tggtccggat attcctcaga atgcgcgccg aagagtcgat cgctcaggcc gcgtaaaatc
      541 gggtacgacc tccgattttt aacggaaaaa gatcagggtg accagaagga aaagaggcag
      601 cagaatagcc gcggaccacg ccatgtagcc gaaaaagctc ggcattttcc acccgcgttg
      661 gtgcgcgatc gaacgcacca tgaaattcgg cgcgttcccg atgtaggtca tggccccgaa
      721 aaagaccgac ccgatactga tcgcggcgag cagtttctcg gggatgttca ggtaagggcc
      781 cgaaagtccc aagccctgcg cgagggagat gaaagtcagg taggtggggg cgttgtcgag
      841 aaaagaagag aacatgcccg tggcccagaa aaactgccag ggctgtttga tgccgagctc
      901 ggcgccccgc agataaagga tctcgagagc gggctgcatg gtgatgaaaa taccgaggaa
      961 aagaatcgcg acttcgagga tggggtcgaa attgaagccg ttagcctgat ggtatgattt
     1021 cggagtgatc ttg
//
LOCUS       DAOOBX010000325         1030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_157399_length_1030_cov_3.163077, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000325 DAOOBX010000000
VERSION     DAOOBX010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157399_length_1030_cov_3.163077"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..>1030
                     /gene="hisG"
                     /locus_tag="PLY30_04605"
     CDS             217..>1030
                     /gene="hisG"
                     /locus_tag="PLY30_04605"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPW65423.1"
                     /translation="MTTHKLKIALPKGSLEEATCNLFRRAGFRISVSSRSYIPSIDDP
                     EIEVVLFRPQEMSRYVEKGIVDCGLTGHDWIVENDSDVVELCELQYSKRTSNPVRWVL
                     CVHESSPFRSVKDLQGKKVVTELVGVTKRYLKKNKVAAEVEFSWGATEAKPPRLADAI
                     VEATETGSSLRANKLKIIDTVITSIPKFIANRQSVGDPWKKHKMDNLVMLLEGAMRAQ
                     EKVGLKMNIEKKNLEKLLVVLPAMKKPTIANLTDENWLGVETIIDESVVRELL"
BASE COUNT          236 a          294 c          307 g          193 t
ORIGIN      
        1 ttccgcccgg cgatttttac gacggtgctg gtcctgatcg tgttcgtcat tttttccaag
       61 gtgcaggaaa aggtcctgcc gccgaagagg gaagagcgtc cctgaccatc ccccatgaac
      121 cgatccaacc tacgaggttg gattcggtgg aaaggggaag gcgcaaggtg accctcagtt
      181 gacaccctga cctcaaaccg ctagaattcg ccacttatga ccacgcacaa gctcaagatc
      241 gcattgccga agggcagtct cgaagaggcg acctgtaacc ttttcaggcg cgccggtttc
      301 aggatcagcg tcagctcccg ctcttatatc ccctccatcg atgatcccga gatcgaggtg
      361 gtgctgttcc gcccgcagga aatgtcccgc tacgtcgaga aggggatcgt ggactgtggc
      421 ctgacgggcc atgactggat tgtcgagaac gattccgacg tcgttgagct ttgcgagctc
      481 cagtactcga aacggacttc caatccggtg cgctgggtcc tctgcgtgca tgagtcgtcg
      541 cctttccgga gcgtgaagga cctccagggc aagaaggtcg tcaccgagct tgtcggggtg
      601 acgaaacgct atctcaagaa gaacaaggtc gccgccgaag tggagttctc gtggggcgcg
      661 acggaagcca agccgccgcg acttgccgac gcgatcgtgg aagcgaccga aacgggatcc
      721 agcctccgcg cgaacaagct caagatcatc gacaccgtga ttacgtcgat cccgaaattc
      781 atcgcgaaca ggcagagcgt cggggacccg tggaagaagc acaagatgga caaccttgtg
      841 atgctccttg aaggcgcgat gcgggcccag gaaaaggtcg ggctcaaaat gaacatcgag
      901 aagaagaacc tcgagaagct cctcgtcgtg cttcccgcga tgaagaagcc gaccatcgcg
      961 aacctgacgg atgagaactg gctcggtgtc gagaccatca tcgacgagtc cgtggtccgt
     1021 gaactgctcc
//
LOCUS       DAOOBX010000326         1029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_157607_length_1029_cov_3.424025, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000326 DAOOBX010000000
VERSION     DAOOBX010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157607_length_1029_cov_3.424025"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1029
                     /locus_tag="PLY30_04610"
     CDS             <1..>1029
                     /locus_tag="PLY30_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65424.1"
                     /translation="IFLLIVYATACVQLYDRVGVAPIQYQCDMLLIALRLGEWNIFLN
                     ELEIVLKGKLDPEGGVRDDLIHDGMRGGYPEKVFSNQLWFNMMFLGVKEKEKDVYYIG
                     EGTFLDRLRNAIDEQLQAAPPERRQKAKENADSAKALIGAFLKDPYGNLHLPDGKWDA
                     SRVDTSDDPDSRSEMRAVEAERAGPDRRAGSFSLQAPSRRAELREEAKVTFGTDLKLG
                     RIQYVPGEKSPWQVWLWRKDKKTDEKKLNILSHKTWWGAMRQALDVLEKEFNDWSVAP
                     VGDDLNLKIARALRNGRIYDLIAIIRKPSWQQVVPAWQAPYEGRRRNANLEARIEALE
                     GLLTEGRD"
BASE COUNT          239 a          248 c          328 g          214 t
ORIGIN      
        1 ccatctttct cctcattgtt tatgcgacag cttgcgtcca gctgtatgac cgtgtgggtg
       61 tcgctccgat tcagtatcaa tgcgacatgc ttctcatcgc gcttcgtctc ggggagtgga
      121 atatcttttt gaacgagctc gagattgttt taaagggaaa actggatccg gaaggtggtg
      181 tgcgggacga tctgatccat gacggtatgc ggggcggata tccggaaaag gtcttctcga
      241 atcagctctg gttcaacatg atgttcctcg gcgtgaaaga aaaagaaaag gacgtctact
      301 acatcgggga agggacattc cttgacaggc tgaggaatgc gatcgatgaa caattgcagg
      361 ctgcgccgcc ggaacgtcgt cagaaagcga aggaaaatgc cgacagcgcg aaggccctga
      421 tcggtgcctt tttgaaagat ccttacggga accttcactt gcctgatgga aaatgggacg
      481 caagccgcgt cgacacgagc gatgatcccg atagccgttc cgagatgagg gcggttgagg
      541 cggaacgcgc ggggccggat cgccgcgccg gcagcttttc gcttcaggct ccttcccggc
      601 gggctgagtt gcgtgaggaa gccaaagtga cttttgggac cgatctgaaa ttaggccgga
      661 ttcaatacgt tccgggggag aagtcgccct ggcaggtatg gctgtggcgc aaggacaaaa
      721 agaccgacga aaaaaagctg aacatcctgt ctcataaaac ctggtggggc gcgatgcggc
      781 aggcgcttga tgttctggag aaggaattta acgattggtc ggtcgcgcca gtcggcgatg
      841 acctgaacct gaagatcgcg agagcgctca ggaacggccg gatctatgat ctgatcgcga
      901 tcatccgcaa accgtcgtgg cagcaggttg ttcccgcatg gcaggcccct tacgagggac
      961 ggcgaaggaa tgcgaacctt gaagcgcgga tcgaagctct cgagggactt ctgaccgagg
     1021 gccgggatg
//
LOCUS       DAOOBX010000327         1028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_157847_length_1028_cov_2.758479, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000327 DAOOBX010000000
VERSION     DAOOBX010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157847_length_1028_cov_2.758479"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..>1028)
                     /locus_tag="PLY30_04615"
     CDS             complement(84..>1028)
                     /locus_tag="PLY30_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPW65425.1"
                     /translation="LNLPGIDLGISAFTARDRECLEFALKNGVDAVSQSFVETAKDIE
                     TVRKAAEAFGKKPFIVAKIERAGALRHLDEILRSADGIMVARGDLGVEVPIEEIAITQ
                     KKMIAKANLAGKPVIVATQMLESMTSSRLPTRAEATDVANAIIDGADCVMLSAESAMG
                     RFPEESVAMLAKIAAFTEANRPRTSLKAIHELCEQDPIDQPGEGESLVVEHAIRTVPA
                     KAIFVPTLSGATVRMISRFKPAPWIIAASQDTAVARGLLFSYGVHTVELAADPESWKD
                     YATNWLTKEFGPGGKAVLLSGPSPRDPSANYRMEFLKF"
BASE COUNT          216 a          308 c          269 g          235 t
ORIGIN      
        1 cttcagggct tccttgacgg cttccttcat gaatttcaag tgttcattcc gggtcataaa
       61 aaactctccg cctcttccgg aagctaaaac ttgagaaact ccatccggta atttgccgag
      121 gggtccctgg gagaaggtcc cgaaagcaaa accgctttcc ccccggggcc aaattctttc
      181 gtcagccagt tcgtcgcgta atccttccag ctctcgggat ccgccgcgag ctcgaccgta
      241 tgaacaccgt aggagaaaag aagtccgcgc gcgaccgccg tgtcctggga cgccgcgatg
      301 atccatggag cgggtttgaa acgcgagatc atccggaccg tagcacccga aagcgtcgga
      361 acaaatatcg ctttggcggg aaccgtccgg atagcgtgtt cgaccacaag cgattcgccc
      421 tctcccggtt gatcgatagg atcctgttcg caaagctcat ggatagcttt caggctggtc
      481 ctgggacggt tggcttccgt gaaagccgct attttcgcga gcatcgccac ggattcttcg
      541 gggaaacgcc ccatcgccga ttcggccgaa agcatgacgc aatcggctcc gtcaataatg
      601 gcattcgcga cgtccgtcgc ttccgcgcgc gtcggcaaac ggcttgaagt catagattca
      661 agcatctggg tggcaacgat cacgggtttt cccgccagat tggctttagc gatcattttt
      721 ttctgggtga tcgcgatctc ttcaatcgga acctccactc cgagatctcc gcgcgccacc
      781 atgattccgt ccgcagacct cagtatctca tcaaggtgcc ggagagctcc ggcacgttct
      841 atcttcgcga caataaaagg tttcttcccg aatgcttccg ccgcttttcg aacggtttca
      901 atatccttcg ccgtctcaac gaaggattga ctcacggcat ccacgccgtt tttgagagcg
      961 aactcgagac attcccggtc gcgggccgtg aaagcgctga tcccgagatc gatcccggga
     1021 aggttcag
//
LOCUS       DAOOBX010000328         1026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_158342_length_1026_cov_2.422245, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000328 DAOOBX010000000
VERSION     DAOOBX010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158342_length_1026_cov_2.422245"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLY30_04620"
     CDS             complement(<1..378)
                     /locus_tag="PLY30_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPW65426.1"
                     /translation="MTNAIHPASVIGRDVVLGADNTIGPNVVIEDGVRIGSGNRILQG
                     AFLGKGTEVGDQNEIHMNAVVGHVPQDLAYKGEETFTKIGSGNVIREFVTIHRGTKAG
                     TSTVIGDGNFIMAYCHIAHNCHLG"
     gene            complement(446..>1026)
                     /locus_tag="PLY30_04625"
     CDS             complement(446..>1026)
                     /locus_tag="PLY30_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPW65427.1"
                     /translation="RPFCADRDGFVLGEGAGMFVVEEYEHARKRGAHIYAEILSHGMT
                     CDAYHMTAPDPEGKEASRAMRLALRNAGIQPEDVDYINAHGTSTPLNDKTETLVIKKV
                     FGDHAYKIPVSSTKSMIGHLIGAAGSVEMIATLLAMENQVIPPTINYHLKDTECDLDY
                     VPNEARPAKIRTAMKNSFGFGGKNSILILRKV"
BASE COUNT          200 a          317 c          271 g          238 t
ORIGIN      
        1 cccgaggtgg caattgtgcg cgatatggca gtacgccatg atgaaatttc cgtctccgat
       61 cacagtcgac gtccccgcct tggtcccccg gtggatcgtc acgaactccc gaatgacgtt
      121 cccggagccg atcttcgtga acgtctcctc gcccttgtac gcgaggtcct gcggcacgtg
      181 tccgaccacg gcgttcatgt gaatctcatt ctgatcgccg acctccgtcc ccttcccgag
      241 gaaggccccc tggaggatcc ggttcccgga accgatgcgg acgccatcct cgatcacgac
      301 attcggtcct atcgtgttgt ccgcgccgag cacaacatcc ctgccgatca cggacgctgg
      361 atggatggcg ttggtcattg ttccggtccc cggggccagc ccctttcaag aaagtttatc
      421 gccaaaacag atgcgttcat cacaatcaga cttttctgag aatcaggatc gaattcttcc
      481 ccccgaaacc gaaggaattc ttcatcgcgg tccggatctt cgcgggacgc gcttcgttgg
      541 ggacataatc caggtcgcac tctgtgtctt tcagatgata gttgatcgtc ggagggatca
      601 cctgattctc catcgcgagc aacgtcgcga tcatttccac acttcccgca gcaccgatca
      661 ggtggccgat catggatttt gtcgagctga cgggaatctt gtaggcgtgg tccccaaaaa
      721 cctttttaat gacgagcgtt tcggtcttgt cattcagcgg cgtcgacgtt ccgtgggcat
      781 tgatgtaatc gacatcctcg ggctgtattc cggcattgcg aagagccaaa cgcatcgcgc
      841 ggctcgcctc ctttccttcc gggtcgggag cggtcatgtg atacgcatcg caggtcattc
      901 catggctcag aatctcggca tagatatgag cgccgcgctt ccgggcatgt tcatactctt
      961 cgaccacaaa cattccggcg ccttcaccga gaacgaaccc gtcacggtcg gcacaaaaag
     1021 gccggg
//
LOCUS       DAOOBX010000329         1023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_158969_length_1023_cov_2.748967, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000329 DAOOBX010000000
VERSION     DAOOBX010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158969_length_1023_cov_2.748967"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..250
                     /locus_tag="PLY30_04630"
     CDS             5..250
                     /locus_tag="PLY30_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPW65428.1"
                     /translation="MITNKLGLHARPAALFVQTANRFESSIEIRKEDMSVDGKSIMGI
                     MTLAAEMGSAIRVTATGKDADEALGALARLVESNFGE"
     gene            255..>1023
                     /locus_tag="PLY30_04635"
     CDS             255..>1023
                     /locus_tag="PLY30_04635"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HPW65429.1"
                     /translation="MKELKGIGVSPGIAIGKVFVLHSGESFNHPKREITQDLIPNEIA
                     RFEDALARTRTEILGIRRKLTDQIGRESSDIFTAHLLILEDRTLIEDVIEVIKNEKVV
                     AEFAFAAVIQRYFQAFSQINDEYLKERISDIRDVGRRLLGNLCGEEKRRLEEFKDKVV
                     IVSHDLSPSDTATLDKSKVLGFVTEIGGPTSHTAILGRSIEIPAIVGAEQITNEVDNG
                     TMAIVDGTHGIVIVDPDQATIDRFSSAETRFIEMTGEL"
BASE COUNT          233 a          289 c          307 g          194 t
ORIGIN      
        1 ccttgtgatc acgaacaagc tcggtctcca cgcgaggccg gcggcgcttt ttgtccagac
       61 ggcgaaccgt ttcgaatcat cgatcgagat ccggaaagag gatatgtcgg tggacggcaa
      121 atcgatcatg gggatcatga cacttgccgc ggaaatggga tcggccatca gggtcacggc
      181 gacaggcaag gacgccgacg aggccctcgg cgcgctcgca aggcttgtcg aatccaattt
      241 cggagaatga aaacatgaaa gaactcaagg gaatcggtgt ttcgcccggg atcgcgatcg
      301 gaaaagtgtt cgtgctccac agcggcgaat ccttcaatca tccgaaacgg gagattaccc
      361 aggatctgat cccgaacgag atcgcccggt ttgaggatgc gctcgcgcgt acgcgcacgg
      421 agattctcgg aatccggcgc aagcttaccg accagatcgg ccgcgaaagt tcggacattt
      481 ttacagccca tctgctgatc ctggaagacc ggacgctgat tgaggatgtg atcgaagtca
      541 tcaagaacga gaaggtcgtc gccgagttcg ccttcgcggc cgtgatccag cggtatttcc
      601 aggccttttc acagatcaac gacgaatatc tcaaggaacg catctcggat atccgggacg
      661 tgggccgccg cctcctcgga aatctgtgcg gggaagagaa gcgacgtctg gaggagttca
      721 aggataaggt cgtcatcgtt tctcacgacc tctctccgtc ggatactgcg acgctcgaca
      781 agtccaaggt gctcgggttt gtcacggaga tcggagggcc gacctcccac acggccatcc
      841 tcggccgctc gatcgaaatc cccgctatcg tcggtgcgga gcagatcacg aacgaagtgg
      901 ataacggcac gatggccatc gtggacggga cgcacggtat cgtgatcgtc gatcccgacc
      961 aggccacgat cgaccggttc tcctccgccg agacccggtt cattgaaatg accggcgagc
     1021 ttg
//
LOCUS       DAOOBX010000330         1019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_159858_length_1019_cov_3.087137, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000330 DAOOBX010000000
VERSION     DAOOBX010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159858_length_1019_cov_3.087137"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLY30_04640"
     CDS             <1..250
                     /locus_tag="PLY30_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65430.1"
                     /translation="MTNMFEDDEPKDWELRDEEPPEALKEKWAKDEILGPGTVPCPSC
                     RKRVPADSMDCLYCGARVLRKAGFLSGLLDRVKGFFSG"
     gene            361..561
                     /gene="thiS"
                     /locus_tag="PLY30_04645"
     CDS             361..561
                     /gene="thiS"
                     /locus_tag="PLY30_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPW65431.1"
                     /translation="MIIRINGKEERIEKTVNLSAFIGARNLRPEGIVVEHNARIVPRE
                     IWETVTLAENDTLEIISFVGGG"
     gene            563..>1019
                     /locus_tag="PLY30_04650"
     CDS             563..>1019
                     /locus_tag="PLY30_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HPW65432.1"
                     /translation="MTDLFRIGGRELRSRLLLGTGKFPSHDVMKKAVEASGADIVTVA
                     LRRVDATSATDNILDFIPKKCVLMTNTSGARNADEAVRIARLARASGAGDWVKIEVIR
                     DNKYLLPENLETLKATEILVKEGFIVLPYMSPDLSMARRMADAGAAAVMP"
BASE COUNT          220 a          288 c          314 g          197 t
ORIGIN      
        1 gatgacgaac atgtttgaag atgatgagcc gaaggattgg gagctccggg acgaagagcc
       61 gcccgaagcc ctgaaggaga aatgggcgaa agacgaaatc ctcggccccg gaacggttcc
      121 gtgcccttcc tgcaggaaac gcgttcccgc ggacagcatg gattgtctct attgcggcgc
      181 ccgcgtcctt cggaaggccg gattcctgag tggtcttctc gaccgggtca aaggtttttt
      241 ctccgggtag gacccgggtt cggggcgggt tgtttccggc ggccattccc gctataattc
      301 ccatatcatt ttctgacccg cgggaatccc tcttgccgca catcttaagg gaacagcttc
      361 atgatcatca gaatcaacgg aaaagaagag cgtatcgaaa aaacggtcaa tctttccgcc
      421 ttcatcggcg cgaggaatct ccgtcccgag gggatcgtgg tcgaacacaa cgcccggatc
      481 gtcccgcggg agatatggga gaccgtgacg cttgccgaga acgacaccct cgagattatc
      541 agctttgtcg gaggagggtg agatgacgga ccttttcagg atcggcggcc gcgaactccg
      601 gagccgcctg ctgctcggaa ccgggaaatt cccgagccat gacgttatga aaaaagccgt
      661 cgaggcttcg ggtgccgata tcgtgaccgt cgcgcttcgc agggtcgatg ccacttccgc
      721 cacggacaac atcctcgatt tcatccccaa aaaatgcgta cttatgacga acacttccgg
      781 cgcccgcaac gccgacgagg cggtccgcat cgcgagactc gcgcgggcga gcggcgcggg
      841 ggactgggtg aagatcgagg tcatccggga caataaatac cttttgccgg agaatctcga
      901 aacgctgaag gcgacggaga ttctcgtgaa agaggggttc atcgtccttc cttatatgag
      961 cccggacctt tcaatggcgc gtcgcatggc ggacgcgggc gcggcggccg tgatgccgc
//
LOCUS       DAOOBX010000331         1018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_160077_length_1018_cov_3.071651, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000331 DAOOBX010000000
VERSION     DAOOBX010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160077_length_1018_cov_3.071651"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLY30_04655"
     CDS             complement(<1..622)
                     /locus_tag="PLY30_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HPW65433.1"
                     /translation="MEKILYLAHTDSNGQLPRIALEVLSAAQKLSKDLAGSTLTAGLI
                     GGDVKSAADQIAGSGAVKIFGVSGADFNESRYATDAKAAEALAKAAGATIVIAPGTSR
                     FARALPGLAFRLKGSIDTHVSGIAVNGAQLDIQRWYYRQRMVATLTRAARPWVLLVDS
                     GAFPACAGAKGSANVEAVAVTLTDADKRTKVTGVQAPSADAQTIRPD"
     gene            complement(632..888)
                     /locus_tag="PLY30_04660"
                     /pseudo
     CDS             complement(632..888)
                     /locus_tag="PLY30_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(904..>1018)
                     /locus_tag="PLY30_04665"
     CDS             complement(904..>1018)
                     /locus_tag="PLY30_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HPW65434.1"
                     /translation="IRFASVELPKQVRQTRIVKDTPVDEIAKEIVAWIQPR"
BASE COUNT          198 a          307 c          297 g          216 t
ORIGIN      
        1 cgtcgggacg gatcgtctgc gcgtcggcgg agggcgcctg aacacccgtc actttcgtgc
       61 gcttgtcggc atccgtcagg gtcaccgcga ccgcttcgac gttcgcggaa cctttcgcgc
      121 ccgcgcaggc cgggaacgcg ccggaatcca cgaggaggac ccacggccga gcggcacgcg
      181 tcagggtcgc gaccatgcgc tggcgataat accaccgctg gatgtcaagc tgggcaccgt
      241 tcacggcgat gccgctcacg tgcgtatcga tcgatccctt gagccggaac gcgagacccg
      301 gcagggcgcg cgcgaaacgg gacgtgccgg gcgcgatcac gatcgtcgcg ccggccgctt
      361 tcgcaagggc ctcagccgct ttggcatcgg tggcgtaacg ggactcatta aaatccgctc
      421 cgctcacgcc gaaaatcttc acggcgcccg agcccgcgat ctggtccgcg gccgacttga
      481 catccccgcc gatcagacct gccgtcaggg tcgaaccggc aaggtccttg gagagtttct
      541 gggccgcgct caacacctca agcgcgatcc tgggaagctg tccgttggaa tcagtgtgtg
      601 cgagatagag tattttttcc ataacacccc tttaccccgt tagagacggg aagcgcttca
      661 agcgattctt gagatagcgt ttccccattc ctgtttttag ataagtttcg cgttcaaagc
      721 atcgttgata tgagaacatg cctcataata gacaagcgcg aaagggccgc gtccttttgt
      781 ggattctgct ctgccccgag catgttcttt gaagcgcttc cgcaaatccc gcgtgaaatc
      841 ggtataaaat tttttatctt tatcgcttcg gatgacacaa ccatgcaccg ttagaaccag
      901 tctctaacgg ggctgaatcc aggcaacaat ttctttggcg atctcatcga cgggtgtgtc
      961 tttgacgatg cgggtctggc ggacctgttt cgggagctca acggaggcga acctgatc
//
LOCUS       DAOOBX010000332         1014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_161084_length_1014_cov_2.549531, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000332 DAOOBX010000000
VERSION     DAOOBX010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161084_length_1014_cov_2.549531"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1014
                     /locus_tag="PLY30_04670"
     CDS             <1..>1014
                     /locus_tag="PLY30_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HPW65435.1"
                     /translation="LAVDGVIAGVGNVVVFLPQIMLLFFFIAFFEDTGYMARAVFVLD
                     RIMKMVGLNGKAFLPLLSSFACTVPGVLATRTIEDRNDRLATILVAPLMSCSARLPIY
                     TLFTAAFIPPGTCFGLLSAQGITLLAMYLLSIGAGLGMAAVFRKTLLKGNRMPFVFEL
                     PPYRMPHLGNVFSVMWDRSKEFLVRAGSIIFLLSIVLWFCVNYPKDPAAEARFAAERA
                     RAESVMSGAELEERLGEIERDQKELQIKSSYAGALGKMIEPAIRPLGFNWEIGIGIIA
                     SFAARELLISTLAIVHHVQGEGEGMTAGLIRALQSTRDPETGRELYTPLVAIGLMVFY
                     VFAC"
BASE COUNT          171 a          280 c          317 g          246 t
ORIGIN      
        1 ctcgcggtgg acggcgtgat cgcgggtgtc gggaatgtcg tcgtattcct cccgcagatc
       61 atgcttcttt tctttttcat cgcgttcttc gaagataccg gatacatggc ccgcgcggtt
      121 ttcgtgctcg accggatcat gaagatggtc ggattgaacg gtaaggcctt tcttccgctc
      181 ctgagttcct tcgcgtgtac ggtgccgggg gtgctcgcga cgcggacgat cgaggaccgc
      241 aatgaccggc tcgcgacgat ccttgtggct cctcttatga gttgcagcgc gcggctcccg
      301 atctacacgc tgtttacggc ggcttttatc ccgcccggga cctgtttcgg attattgtcc
      361 gcacagggaa tcaccctgct cgcgatgtat cttctcagca tcggcgcggg gctgggcatg
      421 gccgcggttt tccgtaaaac tctcctgaaa gggaaccgta tgccgttcgt gtttgaactg
      481 ccgccttacc ggatgcctca tttggggaat gttttttcgg tgatgtggga tcgctcgaaa
      541 gagttcctcg tgcgcgcggg aagcatcatt ttcctgcttt cgatcgtgct ctggttctgc
      601 gtgaactacc cgaaagaccc tgcggcggaa gcccgtttcg cggccgagcg ggcccgggcc
      661 gaatcggtga tgagcggcgc agagcttgag gaaaggctcg gggagatcga acgcgatcaa
      721 aaagagctcc agatcaaatc cagctatgcc ggcgcactcg ggaaaatgat cgaacccgcg
      781 atccgtcctc tcggtttcaa ttgggagatc gggatcggca tcattgcgtc tttcgcggca
      841 cgcgagcttc tgattagcac gcttgcgatc gttcaccatg tgcagggcga aggcgagggc
      901 atgactgccg gattgatccg cgcccttcag tcgacgaggg atccggagac gggacgggag
      961 ctttacacgc cgctcgtggc gatcgggctc atggtttttt atgtgttcgc gtgc
//
LOCUS       DAOOBX010000333         1009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_162194_length_1009_cov_3.591195, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000333 DAOOBX010000000
VERSION     DAOOBX010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162194_length_1009_cov_3.591195"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLY30_04675"
     CDS             complement(<1..301)
                     /locus_tag="PLY30_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017493095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPW65436.1"
                     /translation="MSRDRPAPGERGFTLIELLVVVVITATLASLMIPRFVGQTERAY
                     IAEPHQMLGVIVRAQNDYLDLSDQKKYFRISSEPEECPENSPCAARWARLNLRVPR"
     gene            complement(376..834)
                     /locus_tag="PLY30_04680"
     CDS             complement(376..834)
                     /locus_tag="PLY30_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPW65437.1"
                     /translation="MKLLKRFPGFFFSRSEKSRIVSAIREAEKNTSGEIRVHLERRVK
                     GDILEHARRVFERIGMTKTKDRNGVLIFLAVRDKQVAIIGDRGIHEKVPEGFWDSEIA
                     LMRGCFRERKFAEGVVRAILKAGEKLKAFYPYHDEDQNELADDISVSDWS"
BASE COUNT          224 a          339 c          243 g          203 t
ORIGIN      
        1 tccggggaac cctgagattg agccgcgccc aacgtgccgc gcacggcgaa ttctcggggc
       61 actcttctgg ctcggacgaa atccggaaat atttcttttg gtccgaaagg tccagataat
      121 cgttctgggc cctcacgatc acaccgagca tctgatgggg ctccgcgata taggcgcgtt
      181 ccgtctgtcc cacaaatcgc ggaatcatca gcgatgcgag ggtcgccgtg atgacaacaa
      241 ccaccagcag ctcgatcagc gtgaatcccc gctctcccgg ggccggtcga tctcgggaca
      301 ttaaattcac atcctccctt ttaagggggt tatgctaacg aatcctcggc aacgggacaa
      361 gaaaaacccc gctcttcagg accaatccga tacggaaata tcatcggcaa gctcattctg
      421 gtcctcatca tgataaggat agaacgcctt gagcttctcc cccgccttca ggatggcccg
      481 aacgacccct tccgcaaatt tcctctcccg gaaacacccg cgcatcagcg cgatctcgct
      541 atcccagaat ccctccggca ccttttcatg aatcccgcga tccccgatga tcgcgacctg
      601 tttgtcccga accgcgagga agatcaggac accgttccgg tccttcgtct tcgtcatgcc
      661 gatccgttca aagacccggc gcgcatgctc caggatatcc cccttaacgc gtcgttcgag
      721 atggacacga atttcgccgg acgtgttttt ctcggcctcc cggatcgcgg agacgatcct
      781 cgatttttct gatcgcgaaa aaaagaaacc ggggaatctc ttgagaagtt tcatcaccat
      841 cctccgctcg cgccgccgcc accgaaactg cctccgctga acccgccgaa gccaccgcgg
      901 ctgcctcccg aacggtatcc cgtccccgta taaaagaaac cgccgcgccg tcttccgcga
      961 cgatcatcac caccggagcc ctttcgaagc agaggaggaa gaacgatca
//
LOCUS       DAOOBX010000334         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_163465_length_1004_cov_2.384615, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000334 DAOOBX010000000
VERSION     DAOOBX010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163465_length_1004_cov_2.384615"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PLY30_04685"
     CDS             complement(<1..997)
                     /locus_tag="PLY30_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW65438.1"
                     /translation="MEEGAEPRSEYRSDIPKESKFFKLYGSETVKVNFPVKLLTGEAA
                     AKEEEKDKKPNQFMLVGLADGTMGYLNGKGNISNLDSAVNRPYEDNLYKDGSIKLYFK
                     GLVKGEYLATAQVDTDKDKDELHMFEYVNPEKYYPIYGDSSSSFNEADTRGKFYARID
                     KDDSYGLWGNYNTQEFTQNQLTRYNRTLAGPKGHIELKDWMGEKARKILKPTLDLFYS
                     PSTQEQVSETFSSKGISGPFWLGRTPIVEYTEAIRVETRDKSRSDIILHTVPLVRDVD
                     YEIDYDTGRIFFKEPISSRDENDDPNSIVVDYEYAPLISDKNNYLAGGRLQSTFFD"
BASE COUNT          186 a          257 c          297 g          264 t
ORIGIN      
        1 cgtcaaagaa cgtcgactgg aggcggccgc cggccagata attgttctta tcgcttatga
       61 gcggcgcgta ttcgtaatcc acgacgatgg agttgggatc gtcattttcg tcgcgggagg
      121 agatcggctc tttgaagaaa atgcggccgg tgtcgtaatc gatctcgtaa tcgacgtcgc
      181 ggacaagcgg cacggtgtgg agaatgatgt cggagcgcga cttgtcgcgg gtctcgaccc
      241 ggatggcctc ggtgtattcc acgatcgggg tcctgccgag ccagaagggc ccggaaatgc
      301 ctttcgagga aaaggtctcg gacacctgct cctgcgtaga cggtgaataa aaaagatcga
      361 gcgtgggctt gaggatcttc cgcgcctttt cgcccatcca gtctttgagc tcgatatggc
      421 ctttggggcc ggcaagcgtg cggttgtagc gggtaagctg gttctgcgtg aattcctgcg
      481 tgttgtaatt cccccaaaga ccgtaggagt cgtccttgtc gatccgggcg tagaatttcc
      541 cgcgggtatc ggcttcatta aaggaagagg agctgtctcc gtagatcgga taatattttt
      601 cagggttcac gtattcgaac atgtggagct catctttgtc cttgtccgtg tccacctggg
      661 cggtcgccag gtattcgcct ttcacgaggc ccttgaagta gagcttgatg ctgccgtcct
      721 tgtaaagatt atcttcatag ggcctgttga ccgcggaatc caggtttgaa atattgcctt
      781 tgccgttcag atagcccatc gtcccgtccg cgagacccac cagcatgaac tgattcggct
      841 ttttgtcttt ttcttcttcc ttggcggccg cttctccggt aagcagtttc acgggaaagt
      901 tgaccttgac cgtctcggag ccgtagagct tgaagaattt gctctctttc gggatgtccg
      961 agcggtactc actgcgcggc tcggcgcctt cttccatctc gggg
//
LOCUS       DAOOBX010000335         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_163474_length_1004_cov_2.307692, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000335 DAOOBX010000000
VERSION     DAOOBX010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163474_length_1004_cov_2.307692"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..>1004
                     /locus_tag="PLY30_04690"
     CDS             35..>1004
                     /locus_tag="PLY30_04690"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPW65439.1"
                     /translation="MVVTGCFAETDRDVLERMHEVDMIFSNQGKNRIAEVLTGGGHGS
                     CPGPASQGTEFLPLEIAKSDGRTRANVKIQDGCNHSCSYCKVVLARGRSRSRPPRDVL
                     EEVRRLRDAGYREIILTGIQLGAYGLEKGNEGALAELIRDCAEFEGIERIRLSSVEPT
                     DIRESLIGAFREVPKLCPHVHIPLQSGDDAVLKQMNRRYSPSLYIETISKLREAVPDF
                     CLSLDVMVGFPGETEDAFLKSLEVLKNVRPLKSHIFPFSRRKGTRADRLKILPGTVVR
                     ERVRRISEWSGQISAEVREEFLGREFKVLAEHRDPGTGCWEGYAPNY"
BASE COUNT          219 a          237 c          325 g          223 t
ORIGIN      
        1 ccgccggtta aagcgcgagt atcccgagtg ccggctggtt gtaacgggat gtttcgcgga
       61 aacggaccgg gatgttctcg agaggatgca cgaagtcgat atgattttct ccaaccaggg
      121 gaagaaccgg atcgccgaag ttctgaccgg cggtggacac ggctcatgtc ccggcccggc
      181 gtcgcaaggg acagagtttc ttcctcttga aatcgccaaa agcgacggcc ggacgcgggc
      241 gaacgtgaaa attcaggatg gctgcaatca ttcctgcagc tattgcaagg tcgtgcttgc
      301 ccggggacgt tcgaggagcc gtccgccgcg ggatgttctc gaggaagttc ggcggcttcg
      361 tgacgcgggt taccgtgaga ttattctcac gggtattcag ctcggggcct acggcctgga
      421 gaaagggaac gagggagccc tggcggagtt gatccgggat tgcgccgaat tcgaagggat
      481 cgaaaggatc cgtctgagtt cggtcgagcc gacggatatc cgggagtctt tgatcggcgc
      541 gttccgggaa gtgccgaagc tttgcccgca tgttcatatc cctttgcaaa gcggggatga
      601 tgcggttctc aaacaaatga acaggcgcta ctcgccctcg ctctatatcg aaacgatcag
      661 taaacttcgc gaagcggtgc cggatttctg tttgagtctg gacgtcatgg tcggatttcc
      721 cggtgaaacg gaagacgctt ttttgaaaag cctggaggtt ttaaaaaatg ttcgtccgct
      781 caaaagtcat attttcccgt tcagcagacg gaaggggacg cgtgctgatc gtttaaaaat
      841 tcttcccggc actgttgtcc gggaacgggt tcggcggata tccgagtgga gcggacaaat
      901 ttcagcggaa gtccgggaag aatttttggg acgggaattt aaagtgctcg cggaacacag
      961 ggatccggga acgggttgtt gggaggggta cgctccgaat tatc
//
LOCUS       DAOOBX010000336         1001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Omnitrophota bacterium isolate AMR_MDS_4033
            NODE_164264_length_1001_cov_2.016913, whole genome shotgun
            sequence.
ACCESSION   DAOOBX010000336 DAOOBX010000000
VERSION     DAOOBX010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866004
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Omnitrophota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Omnitrophota bacterium
            Bacteria; Candidatus Omnitrophota.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:47:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 939
            CDSs (total)                      :: 935
            Genes (coding)                    :: 930
            CDSs (with protein)               :: 930
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Candidatus Omnitrophota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164264_length_1001_cov_2.016913"
                     /isolate="AMR_MDS_4033"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2035772"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>1001
                     /locus_tag="PLY30_04695"
     CDS             62..>1001
                     /locus_tag="PLY30_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPW65440.1"
                     /translation="MRRLTSTYSGGFNTSWEIDLWGRIRQKVSSARMEALAAEEFHRG
                     VIITLVADTAQAYFILRELDLELQIAQETFGTRKATHELFRQRQLGGVASDLEVAQAE
                     ADMHETAAKIPDLQRQISIQENRICVLLGREPGPVERGMPIADQARLPEIPSAGLPSD
                     LLRRRPDIIESEYVVKSANAAVGASMGEFLPRLDLVNFIGGEGRSPSDIWGDKTYAWT
                     IGGETKLPLFHGGRNVYNYRAARAQWKQAVSQYQQTVVRAFTDVANALTDIKRLAEVR
                     SAQELQVQADRRAAELSKARYEGGFSSYLEVLDAERR"
BASE COUNT          212 a          267 c          312 g          210 t
ORIGIN      
        1 ccggtcggat cttgtggcgg gcgcccaacg cgaccggaat tccggggttc tttacccaac
       61 tatgagacgt ttgacatcga cctactcggg aggatttaac acttcctggg aaattgatct
      121 ttggggacgt atccgtcaga aagtaagctc ggcccggatg gaggctcttg cggctgagga
      181 gtttcacagg ggggtcatca tcaccctggt ggctgacacg gctcaagctt attttatcct
      241 tcgggaactt gatcttgaac ttcagattgc ccaggaaacc ttcgggaccc gcaaggcgac
      301 ccatgaactt ttccgccagc gtcagctggg cggcgtggcc agcgatcttg aagtggcgca
      361 agcggaagcg gacatgcatg aaaccgccgc gaagataccg gatctccaga ggcagatttc
      421 gatccaggag aaccggatct gcgtgctcct cggacgtgag ccggggccgg tggaacgcgg
      481 gatgccgatc gcggatcagg caaggcttcc ggagattccg tcggcgggat tgccttcgga
      541 ccttctgcga agaagaccgg acattattga atccgaatat gtggttaaat ccgcaaacgc
      601 ggcggtgggt gcttcgatgg gagaattcct tccccgactg gatctcgtga acttcatcgg
      661 cggtgagggg cgaagtcctt ctgatatctg gggcgataaa acctatgcgt ggaccatcgg
      721 cggcgagacc aaactaccgc tttttcatgg cggccgcaac gtttataact accgagccgc
      781 tagagcgcaa tggaagcagg cggtttccca atatcagcag acggtggtgc gcgcgtttac
      841 ggatgtcgcg aatgcgctta cggatatcaa aagacttgct gaagtgaggt cggctcagga
      901 gctccaggtt caggcggacc gccgagccgc ggaactctcc aaggcccgct acgagggcgg
      961 cttctccagt tatcttgaag tgcttgatgc cgagcgccgc c
//
